LOCUS       DAOSLL010000001        25538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_2160_length_25538_cov_5.286191, whole genome shotgun sequence.
ACCESSION   DAOSLL010000001 DAOSLL010000000
VERSION     DAOSLL010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 25538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25538
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2160_length_25538_cov_5.286191"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1477
                     /locus_tag="PLB25_00005"
     CDS             176..1477
                     /locus_tag="PLB25_00005"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOE40010.1"
                     /translation="MKWFVLAAAAALMGGLVTGSVAATDEASMVRGGRLYDHWSREAR
                     VRPPSETQPLFAAKKGSLTGAESWRCVACHGWDYKGSNGFVGIRAAMGKEPLAIVEVL
                     KNPAHRFDELLRERDLLDLANFVSKGQINAQAAIEAWRRSNTAAASFARVYSMVCANC
                     HGADGSQLRDVSPLGDSARQRPLEVMHVLMNGHPGGDMPALSVLGIELAAKMMAYLQT
                     LPTLDLAASVAHGGRLFDNWQTEEKAQRQSLAHPAYPRGGFYASDASLTWRCVACHGW
                     DYQGNQGDYASGRHATGIKGIRAMAGADPARIGAVLRDARHRYDAVLKQRDLQDLANF
                     VSIGQIDMDTLIDRKSRRVRGDAARGSAYFRTLCAGCHGPDGQSAGSPPLGREIRANP
                     WAALHSVLNGHPAEKMPALRELDRQVLADILAHAQGLPDTR"
     gene            complement(1652..2374)
                     /locus_tag="PLB25_00010"
     CDS             complement(1652..2374)
                     /locus_tag="PLB25_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE40011.1"
                     /translation="MNTLQTAAGGICIEGLRKVYGKGANTVEALKNVNMSVAAGEVVG
                     LVGPSGSGKSTLLKCLGAIIEPSSGRMTLGNQVIYDDHWKVADLRALRRDRIGFIFQA
                     PYLIPFLDVTDNVALLPMLAGQPNAQARARALELLTALDVQHRAGAEPSQLSGGEQQR
                     VSIARALANQPPVILADEPTAPLDSERALGVMRILNQMARQFQAAIIVVTHDEKIIPT
                     FKRIYHIRDGQTVEEAGEGRSL"
     gene            complement(2378..3571)
                     /locus_tag="PLB25_00015"
     CDS             complement(2378..3571)
                     /locus_tag="PLB25_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE40012.1"
                     /translation="MISLAGRDILHSWGKFALTGLGLGLLIGVTLSMAGIYRGMVDDA
                     QALLSNSGADLWVVQKDTQGPYAEASSLKDDVVRSVRGMPGIAQAANVTYLTQQVKKA
                     SGDEVRVMVVGIEPGQPGEPAYLLAGRQLTRNHYEAVVDVKTGIGLGEDFSVRRHRYR
                     VVGLTKRMVSSGGDPMVFIPLKDAQEAQFLKDNDSIVNDRTRLSANPAFNRPGVPGLL
                     EAVQSLQTSSHNVNAVLVQLQAGWQPERVAEPLQRWKHVQVFTRAAMEEILVAKLIAN
                     SARQIGMFLAILSVVSAAIVAFIIYTMTLGKIREIAVLKLIGTRNRTIAGMILQQALG
                     LGLIGFVVGKMAATAWAPVFPKYVLLLPQDAVIGLFATLLICALASLLSIRVALQVDP
                     AAAIG"
     gene            complement(3568..4773)
                     /locus_tag="PLB25_00020"
     CDS             complement(3568..4773)
                     /locus_tag="PLB25_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOE40013.1"
                     /translation="MKPNPLLIRRLAIGASALVLLAGVGYVFVRTGPLAPVKVTVAQA
                     TEGSLSPAIFGIGTVEARRAWLVGPTVAGRVLSVKVDVGDAVKAGQLLAEMDPVDLDQ
                     RLAALDAALARAGSAELGAKAQVQDAVARRTLADANARRNQDLARQNFISAGALDARL
                     QEQASAQTAWQVSQANLAGSGQDRQRLQAERAGLAQQRGSVRLQAPADAVVSSRDAEA
                     GSTVVAGQPVLRLLDPASLWIRLRVDQGRSAGLAVGLAASIVLRSQPQTALTGQVARV
                     ELLADSLTEERIAQVAFAALPAGVSVGEMAEVTLQLPATALALLVPNASVQRQQGQTG
                     VWRLNGDRPEFTPVQLGSSSLDGMVQVLKGLDKHDRVVVYSEKALKAGARVKVVAALA
                     ATTDAAGKP"
     gene            complement(4770..5489)
                     /locus_tag="PLB25_00025"
     CDS             complement(4770..5489)
                     /locus_tag="PLB25_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOE40014.1"
                     /translation="MKVTDKPPKQRTEVRQAALIEAALQLAAQRSPADITTGDLAQAV
                     GITQGAVFRHFASKEAIWLAALDWVSQTLMGRLQDAATNCLGHQASANVHLTPACAAA
                     SVGRAAEQPSALAALQAVFMAHVDFVTEHPGVPRVIFQELQRPGDTALKARVGHLLQQ
                     YRQLLAGLLIQAQSEKTIAPHADLQGAAVLFIGSIQGLVMQSLVSGDVADMARQAPAV
                     FAILQRGLQAPSPSLTEKAPR"
     gene            complement(5881..6960)
                     /gene="amrS"
                     /locus_tag="PLB25_00030"
     CDS             complement(5881..6960)
                     /gene="amrS"
                     /locus_tag="PLB25_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HOE40015.1"
                     /translation="MNAAAPYPARYWHWQDDGRMQCDLCPRDCRLHEGQRGACFVRMR
                     LGQQMILTTYGRSSGFCIDPIEKKPLNQFYPGSSVLSFGTAGCNLTCKFCQNWDISKS
                     RDMDALMDLASPDAIAMAAVQHGCKSVAFTYNDPVIFAEYAMDVAAACHARGIQTVAV
                     TAGYMHAPARRDFYASMDAANVDLKAFSEDFYFKLTSAHLQPVLDTLIYLRHETSVWF
                     EITTLLIPGHNDSDEEITAMSRWIMTELGPDVPLHFSAFHPDYKLVDVPATPPATLVR
                     ARNIALRAGLNFVYTGNVHNTEGDTTFCPACQSPLIVRDWYQIRRYRLDALGHCPDCG
                     AAVAGRFEATSGHFGRQRIPIRIGG"
     gene            complement(6957..7526)
                     /gene="amrA"
                     /locus_tag="PLB25_00035"
     CDS             complement(6957..7526)
                     /gene="amrA"
                     /locus_tag="PLB25_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HOE40016.1"
                     /translation="MSIDPDHLNRGQVLLPIARAAISTALRQPMVAPEDAPWLQQSGA
                     CFVTLTQQGQLRGCIGSLEARRSLLADVKANALAAALQDPRFAPLRLTELARTRIEVS
                     LLSPLQAMQFNSQDDALGQLRPGTDGVTFQYGQRRSTFLPQVWEQLPSVAEFMAHLKL
                     KAGLPADFWAQGVLLQRYTVDKWKEGDQQ"
     gene            complement(7516..8340)
                     /gene="amrB"
                     /locus_tag="PLB25_00040"
     CDS             complement(7516..8340)
                     /gene="amrB"
                     /locus_tag="PLB25_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HOE40017.1"
                     /translation="MHQIRQPAVAGAFYPGLAAALASDVQDFLNIARQTTNPASLPVS
                     VPKAIIVPHAGYIYSGSTAALAYALLPAARQLISRVVLLGPVHRVAVRGLALPGADAF
                     ATPLGSVDIDQQAVAAITPLCQVATSAAAHAQEHSLEVQLPFLQTVLGNFQLLPLAVG
                     DATPAEVAEVLEVLWGGPETLIVISSDLSHFLPYHRAQSVDSVTVRSIMHLDACLTHN
                     QACGGTPVNGLLLAARKHQLQAHLLGLCNSGDTAGDKGRVVGYASVAFTQGGQHVD"
     gene            8804..10384
                     /locus_tag="PLB25_00045"
     CDS             8804..10384
                     /locus_tag="PLB25_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE40018.1"
                     /translation="MNLHQLTYLVVDDLELMRAVTVNQLRGMGFDKIKVARHGAEALE
                     VLRTSKIDMVLSDWNMPVMSGLELLKILRADAKLSKMPFLMITAEAERQRIEEVISAG
                     VNGLLVKPYNAGSLKARLERIFNPQKRVLPKPLPPHVASTRADDGPGTGSMLSPSRIL
                     VVDDYPVSLKLLEKLFADEYLVQTATDGATALALCQHEPLPDLVLMDVLMPGMDGFQV
                     VRSLREQPATAQIPVIFVTGLSDDEARIKGMELGAVDFVSKTNDPKALRAKVRNFIHF
                     VDIRRKLQLDYDAMLDAARSREEAENITRHDLKGSLAGIVGMVQSLADDGDMHPRHTA
                     QLRLVQQTAEQVMNMVNLSGELYKIEMGRFKLKTTAVDIGEILHRIVVLSRAGFADKR
                     LTISVDTDSPVGAELPKAQADAMLCYSLFQNLVKNACEAAPPDSRVEVLLRDESPLRI
                     LVSNMGAVPPELRERFFDKFATSGKAGGSGIGTYSAQLLAHAQNGSISMVTDDANNCT
                     TLTVTLPRHAFDAAHSNA"
     gene            10409..10987
                     /locus_tag="PLB25_00050"
     CDS             10409..10987
                     /locus_tag="PLB25_00050"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HOE40019.1"
                     /translation="MGNRLTKIATRTGDNGSTGLGDNTRVSKNSLRVHAMGDVDELNS
                     NIGVLLCEDMPADLRSLLVEIQHQLFNLGGELSIPGFELLKAEAVLALDQALLEYNAH
                     LPILQEFILPAGTRAASLAHVCRTVARRAERAVVALGDQDAIKDTPRHYLNRLSDLMF
                     VLARVLNRFRSDGSTGDDVYWRSERLAQTADN"
     gene            complement(11040..11258)
                     /locus_tag="PLB25_00055"
     CDS             complement(11040..11258)
                     /locus_tag="PLB25_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HOE40020.1"
                     /translation="MFSEYREQIVQIKAEDAHFAQIFDQHDALDAKIKRMESHVEPAT
                     HLEIEKLKKEKLHLKDAVYAVLRKAKQA"
     gene            11539..12780
                     /locus_tag="PLB25_00060"
     CDS             11539..12780
                     /locus_tag="PLB25_00060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOE40021.1"
                     /translation="MKLLRSSLIKAGAVWVLACLALPGCSDKTATAGDPAVAGASAAA
                     SVPAIAASSAAPPVSVTIATATMRDMAVNLRATGTVVPLTSVDVRSQVTSTVRQVHFK
                     EGQFVKAGQLLFTLDVRADEAGLARARAQLARDQVSLADARRQHERASQLLAQNFVSQ
                     GAVDTAQAQVDGWRATVAADEAGVAASQVVLSYGRITAPNAGRAGAVNVFAGSAVQAN
                     QTPLVTITQLDPISVSFSIAQRDLSSALAALGDGGAVVQATLADGGGGFRGRLQFIDN
                     LVDASSGTIKAKAVFDNPAARLWPGAFVEVSQTVSTLAGAVVLPQACIIQSIRGAIVY
                     VIADGVATVRPVKLLYAQGGEAAVTGVKAGEAVALDGRQNLRPNALVVVRAPASAAAG
                     DASGKGPGSGSGPGTKKSSQP"
     gene            12777..15875
                     /locus_tag="PLB25_00065"
     CDS             12777..15875
                     /locus_tag="PLB25_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOE40022.1"
                     /translation="MNLSEVFIRRPVMTVLLNLAIVLAGVIGFRSIPIAALPSYDTAV
                     ISVSAALPGASPETMAISVALPLEKQFQTVSGLKTISSTSTLGNTSLTLEFEEGRDID
                     AAALDVQAALLRAQRALPDDMSNPPSYRKVNPADAPVLLISLRSPSLAPADLQDYAEH
                     LISPTLATISGVAQVNIFGAKRYAVRVRVQPDALVARNIGLDEVSSALRAANVNTPVG
                     TLDGPTQTLVLQANKQLRNAAEFAELIISTRGGNPVRLRDVARVEDSLESLKSWATFN
                     GEPTTTLAILRQPGANTVQVVDAIRAALPGLQAQLPKSVSLTPINDRSLSVREALHDV
                     TLTLLGTIALVVLVIFLFLRRFVATVIPMLSLPVSLIGAVALLWGLSYSLDNISLLGL
                     TLAVGLVVDDAIVVLENIIRHVERGENPFQAALRGAREVGFTILSISVSLIAVFIPIF
                     FMPGVIGLLFHEFAVVVGLSIVVSAFVSLTLVPMLASRFLTDEAHKKPPGALVRVFEH
                     GFDWMLAGYTRWLDRALAHRRLILALASATIVATVWLAMVIPKGFFPEEDLGQMSVTT
                     EAGEDTSFTEMVRLQERAAEIIRADPAVAALNSFNGGSGAQNTGRMFITLKPRAERPP
                     MNKVVEGLRKKLNNLPGIRLFMRPVQNLQLGGRPSKAQFQYILQSVRADELNDWANKL
                     QERLRVDPLFRDVTSDAQLRGLQAQLKIDRDRANALGVSIEGIRTALYSAFGERQVST
                     IYLPTDSYQVIMEVAPEARQDESAIAGIYVRASTGTLVPLSSFTTVERSVGPTSINHV
                     GQLQAVTVSFNLAPGAALGDATTKIDAAREAIQMPASIISTYGGDAAVFKSSQASQMV
                     LILAALLVIYVLLGVLYESFIHPITILMGLPSAAVGALATLMLFGQDLTLIATIGIVL
                     LIGIVKKNAIMMIDFALDAQRHQGMAPAQAIREACILRFRPIMMTTMAALMGALPIAL
                     GLGAGSELRQPLGLAVVGGLVFSQAITLFITPVIYLALDRFSGHGPVLTPEVPVGQDD
                     AA"
     gene            16094..16330
                     /locus_tag="PLB25_00070"
     CDS             16094..16330
                     /locus_tag="PLB25_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40023.1"
                     /translation="MAILSMTLLTMLVVLVTMLTIRIIQSMLRGQTCVPGCASKDQRG
                     AGWQAPGFGCALKALSIMKHRAQCPSEVEIKMRA"
     gene            16464..17774
                     /gene="glgC"
                     /locus_tag="PLB25_00075"
     CDS             16464..17774
                     /gene="glgC"
                     /locus_tag="PLB25_00075"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOE40024.1"
                     /translation="MAEFEESSFGKRSARDPRFVSQLTRNTFALILAGGRGSRLHELT
                     NWRSKPAVPFGGKFRIIDFTLSNCVNSGVRRVGVATQYKAQSLIRHLQLGWSFLDGRL
                     GEFIEIMPAQQQMDDSHWYQGTADAVFQNLRELRIANPEYVLILSGDHIYRMDYGRIL
                     AAHVEHKADLTVACIEVPLKGASEFGVMTVNEEGRIVDFAEKPINPQPMPGNPDMALA
                     SMGIYVFNARFLFEQLLRDADDPKSSHDFGKDIIPHSVKRYRTYAHSFADSCVYEPGK
                     AIYWRDVGTLDAYWAANMDLVHVTPDLTLYDDTWPIWTWQPQSPPAKFVFDDDTRRGQ
                     AVDSMVSGGCIISGATVRRSMLFSCVNVHSYSLVEESIVLPNVSIGRNCVIKKCILDK
                     NCIIPDGTQIGVNLEDDKKRYHVTESGITLVTEAMLGQGKVNIR"
     gene            complement(17823..18170)
                     /locus_tag="PLB25_00080"
     CDS             complement(17823..18170)
                     /locus_tag="PLB25_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HOE40025.1"
                     /translation="MHEASLAGGILQLVEDSAKREGFKRVTVLTLEVGKLANIEVQAL
                     KFSLEAIAPGTLLEGARLVFEEPEGQAWCMNCSQTVPLAERGLACPQCGSYQLRPNAG
                     LSLRVIDMLVLDL"
     gene            complement(18163..18660)
                     /locus_tag="PLB25_00085"
     CDS             complement(18163..18660)
                     /locus_tag="PLB25_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HOE40026.1"
                     /translation="MLQAHKTPLPVLVLGWGNLSRGDDALGPLCVAALQGQFRDALSN
                     SIEFLDDYQLQVEHTLDLLGRRQVLLIDACLDCAAPFEVRQPQPYPDHSYTSHALTPS
                     ALLQVFSDVHGQVAPATTMLAIRGESFELGQAMSPAAQTNLAAALIWAKAWLESAILQ
                     EPVSA"
     gene            complement(18721..20238)
                     /locus_tag="PLB25_00090"
     CDS             complement(18721..20238)
                     /locus_tag="PLB25_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HOE40027.1"
                     /translation="MSFDLETAANPKGLRRVAIDPVSRVEGHGKVTLLLDDDNQVQQA
                     RLHIVEFRGFEKFIEGRPYWEVPVMVQRLCGICPVSHHLAASKAFDRIIGAMPITASA
                     EAVRRLMHYGQMLQSHALHFFHLSSPDLLFGFDSPVAQRNIVGVVQANPEVAKKGILL
                     RKFGQEIIRITSGKRVHGTGAVPGGINKLVTAAERDALKNELPLMLQWAQDSVDLAKQ
                     LHAQSEALYNQFGSFRSNLLSLVRSDGAMDLYDGVLRVRDANGMILFDGVSDQNYLEL
                     IEEEVRSWSYMKFPHLKSLGRELGWYRVGPLARVQNCDFIPSPLAEAERQEFVAWGKG
                     GLVHASLAYHWARMIEMLHSVEVIGQLLDDPALLSGDLLTSGNRLRSGVGIIEAPRGT
                     LIHDYDVGDDDLVTRCNLIVSTTHNNQAMNEAVRSVARDYLNGHEITEGLLNHIEVAI
                     RAYDPCLSCATHALGQMPLDVVLLSSDGSVLDRVQKSSAGGLSRPTGNFFNENAL"
     gene            complement(20535..21125)
                     /locus_tag="PLB25_00095"
     CDS             complement(20535..21125)
                     /locus_tag="PLB25_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HOE40028.1"
                     /translation="MNAFTYGSDLAGSPAPAARKLKIATVSLAGCFGCHMSLLDIDER
                     LLDLLELVEFDRSPLTDIKTIGRCDLVLIEGGLCNAENVHVLREFRAKCKILVAVGAC
                     AINGGLPAQRNQRDVGKMMRDVYCRQAGGSIGNQIPNDPELPLPLNHVHPIHEVVHID
                     YFLPGCPPSGDAIWTFLTDLLAGRTPHLGHGLIHYD"
     gene            complement(21122..21856)
                     /locus_tag="PLB25_00100"
     CDS             complement(21122..21856)
                     /locus_tag="PLB25_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOE40029.1"
                     /translation="MSYYDRIDALSPGTFSLDGDEIEFGPGESILQAATRAGYYIPHL
                     CWHPEFGAKGSCRLCTVRINGRMGAACTVQAASGQEVESNTDDLNAQRKTLLQMIFIE
                     GNHFCPSCEKSGNCRLQATAYDIGMEGPHFEEFYPDRRVDASHPDMLLDLNRCILCSL
                     CVRASHEVDRKDVFAIGGYGIKTHLLINSPSGQMVDSALLPSDRAANVCPVGAILHKR
                     KGFVIPIGQRRFDKVPVSGQIDENKP"
     gene            complement(21853..23643)
                     /locus_tag="PLB25_00105"
     CDS             complement(21853..23643)
                     /locus_tag="PLB25_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HOE40030.1"
                     /translation="MNPNALDLESLLERHQRAPHRLVQILREIQAQLLWLPRDTLTAV
                     ASALGLSLAHVEGVAGFYRFFHSQPVGQYRVLFSDNITDRMQGSEEMRRHLRHLLKVE
                     HHAMRADGRVSVAATSCTGMCDQGPAALVNHHQLVTRLTPGRIDHMADLIEAQVPTHL
                     WPAEWSRVDDQIRLANVKLGAQPAPGQAIAAALARGAQATLDDLKRSKLRGRGGAGYA
                     TGTKWQLCRNTRSETHYVVCNADEGEPGTFKDRVLLTSYADTVFEGMTIAALIVGARQ
                     GLVYLRGEYHYLLDSLKATLQRRRQQGLLGQSIQGQAGFDFDIDIHVGAGSYVCGEES
                     ALIESLEGKRGTPRIRPPFPVERGYLGQPTTVNNVETFCAVTHIAIHSGAWWAAMGTP
                     QSTGTKIHSVSGDCDRPGIYEYPFGTRIGRILEDCGARDTQAVQVGGPSGVCVSAMEF
                     GRRIGFEDVPTAGAFMVFDRSRNMFDVANNFVQFFAHESCGFCTPCRVGTELVMRRMD
                     KLKRGYGSSDDIQVLYELDKLMHGSTHCGLGAAACNPLRDTIAKFRPAYEQHLKSMHF
                     APGFDMDAELSQARLATGRDDIGAHLETLT"
     gene            complement(23733..>25538)
                     /locus_tag="PLB25_00110"
     CDS             complement(23733..>25538)
                     /locus_tag="PLB25_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE40031.1"
                     /translation="DIAHIEFSVEDTGIGIAAANQQHIFDGFSQAEASTTRRFGGTGL
                     GLSISRRLVALMGGELTLESQLGIGSTFAFTVTLPVAPEEPKAATTSPALPSGLRVLV
                     VDDNPTARSVLASMAESLEWQVDQAAGGADALWLIDQRASSGQDPYQAIFVDWQMPGM
                     DGWETIGRMQQENRNSHSPVVVMITANGREMLAQRSVEEQTRLNGFLVKPVTASMLYD
                     AVVDALADNGHPQTKATANAAPQPLLGMHLLVVEDNMVNQQVAQEMLTNEGALVELAD
                     NGQVGVDAVFGAIADGKPFDAVLMDIQMPVMDGYSASRVLRQNAGLADLPIIAMTANA
                     MDSDRDACLAAGMNAHVGKPFKLVDLVALLQQLTRRPSAESMTGEGSMVPHLMPNQLP
                     EPALPRIDAVDTVGALDRLGGNSALYVRVLQSYLTDIGGLPEQLDIALNKSDWESAEK
                     ILHALKGLSATVGASYMAAVARKAELVIKKTKAASKDAAPLDVRAQSISFSAAVAATQ
                     IVMGQVANTCAPGTIDQHVATPDADLAPDGHEMLRQLQVLLRSSDMAAVSAFEQLQLN
                     SALTSVDEFKQLTRAMATFDFAQAAEVCGLLLQRN"
BASE COUNT         5096 a         7535 c         7657 g         5250 t
ORIGIN      
        1 gagcagcttg gcaaactcaa gctcaagtac tgacgcttga gtcggcgccg catcgttttg
       61 ccgacgttgc catgttgatg tgaagccgtt gcacaccagc ggctttttta cttttgcagc
      121 agtctgcccg gtggcttttt ggtcagtccc tgctagccag aagaaaggcg ggcgtatgaa
      181 atggtttgtg ttggccgctg cagcggcgct gatgggtggc ctggttaccg ggtcggtggc
      241 ggcaaccgat gaagcttcca tggttcgcgg cggtcgcttg tatgaccatt ggagccgcga
      301 agccagggtt cggccaccca gcgagacaca gcccttgttc gcagccaaaa aaggcagttt
      361 gaccggggcc gaaagctggc gctgcgtggc ttgccatggc tgggactaca agggcagcaa
      421 cggttttgtc ggcatccgtg ctgctatggg caaagaaccg ttggccatcg tcgaggtgct
      481 caaaaacccc gcgcaccgct ttgatgaact gctgcgtgag cgtgacttgc ttgatcttgc
      541 caactttgtc agcaaggggc aaatcaatgc gcaggcggcg atcgaggcct ggcggcgctc
      601 aaacaccgct gctgcatcct ttgccagggt gtacagcatg gtgtgtgcca attgccatgg
      661 cgccgatggc agtcagttgc gggatgtgtc accgctgggg gattcggccc ggcagcgacc
      721 tcttgaggtg atgcatgtgc tgatgaatgg gcatccaggc ggcgacatgc cggcgctgag
      781 cgtattgggc attgagcttg ccgccaaaat gatggcgtac ttgcaaaccc tgccaacgct
      841 cgatctggcg gcatccgttg cgcatggtgg tcggcttttt gacaactggc agacggaaga
      901 aaaagcgcag cgccagtcgc tggcgcaccc ggcctatccg cggggcgggt tttatgccag
      961 cgatgcatcc ctgacctggc gctgtgtggc ttgccacggc tgggactatc agggcaatca
     1021 gggtgactat gccagcggtc gccatgccac gggcatcaag ggcatccgcg ccatggccgg
     1081 tgctgacccg gcacgcattg gggcggtttt gcgtgatgcc aggcaccgct atgatgccgt
     1141 cctgaaacag cgtgatctgc aggatctggc aaattttgtc agtatcgggc agatcgatat
     1201 ggataccctg atagaccgca aaagccgtcg cgtgcgtggt gacgcagcgc gtggcagtgc
     1261 ctactttcgc accctttgtg ctggctgcca tgggccggat ggccagtccg ctggctcacc
     1321 gccgttgggt cgcgagatca gggccaaccc ctgggcggcg ctgcattcgg tgttgaatgg
     1381 gcatccggcc gaaaagatgc cagccttgcg tgaacttgat cggcaagtgt tggctgacat
     1441 cctggcccat gcacagggct tgccggacac ccggtaagga tgtcgtcggg aataactccc
     1501 cgttttgcac caaggagttg ggatgcacgg ctaggcgcaa gacgcgccgt ttagctggct
     1561 aaacaagcgt cttgcaacga cgctgtgcgc ccaaatccgc cggtgcaaat aggggaagtt
     1621 atttctgacc acatcctaac tggctggcgg gtcaaagtga gcgcccttcg ccagcctctt
     1681 ccaccgtctg cccgtcgcga atgtggtaaa tccgtttgaa ggtcggaatg attttttcgt
     1741 cgtgtgtcac tacgatgatg gcggcctgaa actggcgcgc catctggttg aggatgcgca
     1801 tgacacctaa ggcccgttcg ctgtcgagtg gcgcggtggg ctcgtcggcc agtatcactg
     1861 gcggttggtt ggccagcgcc ctggcaatcg acacgcgctg ctgttcaccg cctgagagtt
     1921 gtgacggttc ggccccggcg cggtgctgca cgtccagcgc cgtcagcagt tcgagcgccc
     1981 gcgcccgtgc ttgcgcgttg ggttgacctg ccagcatggg cagcagcgcg acgttgtcgg
     2041 tgacatccag aaacgggatc aggtacggtg cctgaaagat gaagccaatg cggtcacgcc
     2101 gcagcgcacg caggtcagcc actttccagt gatcgtcgta aatcacctgg ttgcccaacg
     2161 tcatgcgccc cgagcttggc tcaatgatgg caccaagaca tttcagcagg gtgctcttgc
     2221 ccgagccaga cggccccacc agcccgacca cctcacctgc tgccaccgac atgttgacgt
     2281 ttttcagtgc ctcgaccgtg ttggccccct tgccataaac cttgcgcaaa ccttcgatgc
     2341 agatgccgcc agcggcagtt tggagtgtgt tcatggttca gccaatcgcc gctgccgggt
     2401 ccacttgcag ggcgacgcga atcgacagca ggctggccag ggcgcagatc agcagcgtgg
     2461 caaacaggcc aatcacggca tcttgcggca gcaacagcac gtatttcgga aacaccggtg
     2521 cccaggcggt ggccgccatc ttgccgacga caaaaccaat cagccccagg cccagcgcct
     2581 gctgcaagat catgccggca atcgtgcggt tgcgtgtgcc gatcagtttc aagaccgcaa
     2641 tttcgcgaat tttgcccagc gtcatggtgt aaatgatgaa ggccacaatg gccgcgctga
     2701 ccaccgacag aatggccagg aacatgccaa tttgccgggc tgaattggca atcagcttgg
     2761 ccaccagaat ctcttccatc gccgcgcggg taaacacctg cacatgtttc cagcgttgca
     2821 agggttctgc cacccgctcg ggctgccagc cagcttgcaa ttgcaccagc accgcgttca
     2881 cattgtggct gctggtttgc aggctttgga cggcttcaag cagacccggc acgccggggc
     2941 ggttgaaggc cgggttggct gacaagcggg tgcggtcgtt gacgatgctg tcgttgtcct
     3001 tcaaaaactg tgcctcttgc gcgtctttca aagggatgaa caccatcggg tcgccgccag
     3061 acgacaccat gcgcttcgtc aggccgacca ccctgtagcg gtggcggcgc acgctgaagt
     3121 cttcgcccag gccgatgccg gtcttgacat cgaccaccgc ctcgtagtgg ttgcgcgtca
     3181 gttgtcggcc agccagcagg taagcgggtt caccgggctg gccgggctca atgcccacca
     3241 ccatcacccg cacttcgtcc ccgctggctt ttttcacctg ttgtgtcagg taggtgacgt
     3301 tggctgcctg ggcaatgccg ggcatgcctc gcacactgcg caccacgtcg tctttcaggc
     3361 tggaggcctc ggcgtagggg ccttgggtgt ctttctgcac cacccacagg tcggcaccgc
     3421 tgttgctcag cagcgcctgc gcgtcatcaa ccatgccacg gtagatgccc gccattgaca
     3481 gcgtgacacc aatcagcaaa cccagcccga gcccggtcag ggcaaatttg ccccaactgt
     3541 ggagaatgtc acggcctgcc agactgatca tggcttgcct gcagcatctg tggtggcggc
     3601 cagggcggcc accaccttga cacgggctcc ggccttgagc gctttttcgc tgtaaaccac
     3661 cacccggtca tgtttgtcca gccccttgag cacctgcacc ataccatcca gactgctgct
     3721 gccaagttgc accggggtga actccggccg gtcgccgttg agtcgccaga ccccggtttg
     3781 cccctgctgg cgctgcacac tggcgttggg caccaacagg gccaaggcgg tggctggcaa
     3841 ctgcagcgtg acttcggcca tttcgccgac cgatacgcca gcgggcagtg cggcaaaagc
     3901 cacttgcgca atgcgctctt cggtcaggct gtcggccagc aactcaaccc gcgcgacctg
     3961 gccggtcaag gcagtctggg gctgcgagcg cagcacgatg ctggccgcga gccccaccgc
     4021 cagtccggca gagcgaccct ggtcaacccg cagtctgatc cacaggctgg ccgggtccag
     4081 caggcgcagc accggctggc cagccaccac agtgctgcca gcttctgcgt cccggctgct
     4141 gaccacggca tcggccgggg cctgcagacg cacactgcct cgctgctgcg ccagaccggc
     4201 gcgctcggcc tgcaagcgct gtcggtcttg cccgctaccg gccagattgg cttgcgagac
     4261 ttgccacgcg gtctgcgccg aggcctgctc ctgcagtctg gcatccagcg cacccgcgct
     4321 gatgaagttt tgccgggcca ggtcctgatt gcgccgggca ttggcatctg ccagcgtgcg
     4381 ccgcgcgact gcatcctgca cctgtgcctt ggcgcccagc tcggcgctgc cagcgcgcgc
     4441 cagcgcggca tccagggccg ccagccgctg atcaaggtcc accgggtcca tctcggccag
     4501 cagttgcccg gccttgacgg catcacccac gtcgactttc acgctcagca cccgacccgc
     4561 caccgttggg ccgaccagcc aggcgcgcct ggcctcaacc gtgccaatgc caaaaatggc
     4621 cgggctcaga ctgccctcgg tggcttgcgc cacggtcacc ttgaccgggg cgagcggacc
     4681 ggtgcgcaca aacacatagc ccacgccagc caacaggacc agcgccgacg cgccaatggc
     4741 caaacggcga atcaacagag ggttgggttt catcggggtg ctttctcagt caaagatggg
     4801 ctgggtgcct gcaggccgcg ttgcaaaata gcaaatacag ccggtgcctg gcgtgccatg
     4861 tcagccacat cgccagacac cagtgactgc atcaccaggc cttgtatgga accaataaat
     4921 agcactgcag cgccctgcag gtctgcgtgg ggcgctatgg ttttttctga ctgggcctgg
     4981 atcagcaggc ctgccagcaa ctggcggtac tgctgcagca ggtggcccac ccgtgctttc
     5041 agggcggtgt cgccgggcct ttgcaactcc tgaaaaatca cccgtggcac gcccggatgc
     5101 tcggtcacga aatcaacatg ggccatgaat accgcttgca gggcggccag tgccgacggc
     5161 tgttcggctg ccctgccgac cgacgctgcg gcgcaggcgg gagttaaatg cacgttggcg
     5221 ctggcttggt ggcccaggca gttggttgca gcatcctgca ggcgacccat caaggtttgc
     5281 gacacccagt caagtgcggc cagccagatt gcctctttgc tggcaaagtg ccggaacact
     5341 gcgccttgcg tgataccaac cgcctgcgcc aggtcgccgg tggtaatgtc ggccgggctg
     5401 cgttgcgccg ccaactgcag ggcggcttcg atcagggcgg cctggcgcac ttcggtgcgc
     5461 tgcttgggag gtttgtcggt gactttcaat gtggcttaag taattgagct attactttaa
     5521 gtataccgcc ccctgccaaa ctgggttgct gcgtgttccg aagaaataga taatgcgtta
     5581 acaatcaaaa ctatagctag taacgcagtc tgcataaggg ctggatgccg atttggcaga
     5641 tgaaaccccg gcagcttgcg ccgtcggcct agcccatgtt cctttgagaa cgcctgcccc
     5701 gcgtgcgggg ttgggatgct caatggcagg acggatgcgc atgcgggctc ctgttgactg
     5761 caccatttag tgaccctttt agtgctttag cccatggtgg cattgcgtca ccagctatca
     5821 aacatgaagc accctggagt cagccagcgt tgcgctgcca ccggtcctgc tggccggcat
     5881 tcagccgccg atgcgaatcg gaatgcgctg gcggccaaag tggccactgg ttgcctcaaa
     5941 ccgtccggcc acggcagctc cgcagtccgg gcaatggcca agggcatcaa gccggtagcg
     6001 cctgatctga taccagtcgc gcacgatcaa tgggctttgg caggccgggc agaaggtggt
     6061 gtcgccctcg gtgttgtgga cgttgccggt atagacgaag ttcagcccgg ccctcagggc
     6121 aatgttgcgg gcgcgcacca acgtggcggg cggtgtggcg ggcacatcga ccagcttgta
     6181 atcggggtga aaggcggaaa agtgcagcgg cacgtccggc ccgagttcgg tcatgatcca
     6241 gcggctcatg gccgtgatct cctcgtccga atcgttgtga cccggaatca acagggtggt
     6301 gatctcgaac cagacactgg tttcgtgcct gaggtagatc agggtgtcga gtaccggctg
     6361 caaatgggca ctggtgagct tgaagtagaa gtcttcgcta aaggctttga ggtccacatt
     6421 ggctgcgtcc atgctggcgt agaagtctcg ccgggccggt gcatgcatgt aaccggcggt
     6481 gacggccacc gtctggatgc cacgggcatg gcaggcagcg gcgacgtcca tcgcgtattc
     6541 ggcaaaaatc accgggtcgt tgtaggtgaa ggcaacgctc ttgcagccgt gctggacggc
     6601 ggccatggca atggcatcgg ggctggccag gtccatcagc gcgtccatgt cgcgcgactt
     6661 gctgatatcc cagttctggc aaaacttgca agtcaggttg caacccgccg tgccaaatga
     6721 cagcacgctg ctgccagggt aaaactggtt gagcggcttt ttctcgatcg ggtcgatgca
     6781 aaagcccgat gaccgaccgt aagtggtcag gatcatttgc tggcccaagc gcatgcggac
     6841 aaaacaggca ccgcgctggc cttcgtgcaa gcggcagtca cgcggacaca ggtcgcactg
     6901 catgcggcca tcgtcctgcc aatgccagta gcgcgcaggg tagggcgctg cggcgttcat
     6961 tgctggtcgc cttctttcca cttgtccacg gtatagcgct gcaacagcac accctgcgcc
     7021 cagaagtcgg cgggcaagcc agccttgagc ttcaggtgcg ccatgaattc ggcaacgctg
     7081 gggagctgct cccacacttg cggcaaaaag gtgctgcggc gctggccata ttgaaacgtc
     7141 acgccatcgg tgccggggcg caactggcca agcgcatcgt cctgactgtt gaactgcatg
     7201 gcttgcagtg gcgacagcag ggacacctca atccgggtac gggccagctc ggtcaggcgc
     7261 aatggcgcaa accgggggtc ttgcaaagcg gcagccagtg cattggcctt gacatcagcc
     7321 agcagactgc ggcgtgcttc gagcgaacca atgcagccgc gcaactggcc ttgctgggtc
     7381 agcgtgacaa aacaggcgcc gctttgctgc agccaggggg catcttcggg ggccaccatc
     7441 ggctgcctga gggcggtcga aatggcggca cgggcaattg gcagcaatac ctgaccgcga
     7501 ttgaggtggt ctggatcaat cgacatgctg gcccccttgc gtgaacgcga ccgaggcata
     7561 acccaccacc cggcccttgt cgcccgccgt gtcgcctgaa ttgcacagcc ccagcaggtg
     7621 cgcttgcaac tggtgttttc gggccgccag cagcaggccg ttcaccgggg tgccgccgca
     7681 ggcctggttg tgggtcaggc aggcatcaag atgcatgatg cttcggaccg tcacactgtc
     7741 caccgactgc gcccggtggt agggcaaaaa gtgcgacaaa tccgagctga tgacgatcag
     7801 ggtctctggg ccaccccaca gcacctccag cacctcggcc acttcggcag gtgttgcatc
     7861 ccccacggcc agcggcagca gttggaagtt gcccagcacc gtttgcaaaa atggcagttg
     7921 cacttccagc gaatgctcct gcgcgtgggc ggccgcactg gtcgccacct ggcacagcgg
     7981 ggtgatggcc gcgaccgcct gttggtcgat gtcaaccgaa cccaagggtg tggcaaaggc
     8041 atcggcaccc ggcagcgcca gtcccctgac tgccacccgg tgcaccggcc cgagcagcac
     8101 cacgcgagag atgagctggc gcgctgcagg cagcagtgcg taggccagcg cagccgtcga
     8161 gccactgtaa atatagcctg catgtggcac gatgatggcc tttggcacgc tgaccggcag
     8221 gctggccggg ttggtggtct gccgggcgat gttcagaaaa tcttgcacat cgcttgctaa
     8281 agcagccgcc aatccaggat aaaaggcacc cgcaacagcg ggttgacgga tttgatgcat
     8341 gcacagctcc attcaaaaag taactgcagc ttaggccaat ttgaatcagg ctggtttgcg
     8401 gtttgtcaag tcaacccaag cacgttgcgc gtccatggtc ccggattcct cggttgaccg
     8461 tttgtttttt tctgtaagtc cttatgcgca tctacattat tggttttgat cgggttcacc
     8521 cattgggtga cgcgtgatgc gctatgaacc cgattgaatc gtcggcaaag cgcctgggga
     8581 cgttgccgct cctggcgggc agaagagctg tgctttgatg tcgcccgtgc agggcgtagc
     8641 gatgccacaa ggctggcctg cttcaagccg tccaaaccta cgaaggcacg ttttgagctg
     8701 cggcttttgc cttgcccagc cgcgccgacg gctgcccact cgggcgcgtc caacttaatc
     8761 taagatgata atcttcccat atgaagtgtc tgtcgccatg tccatgaacc tgcatcaact
     8821 cacctacttg gtggtggacg atctggaact gatgcgggct gttaccgtca accagttgcg
     8881 tggcatgggc ttcgacaaaa tcaaggtggc gcgtcatggt gccgaagccc ttgaggtgtt
     8941 gcgcaccagc aagattgaca tggtgctgtc agactggaac atgccggtga tgtcggggct
     9001 ggaactgctc aagatcctgc gtgccgatgc caagctgtcc aaaatgccgt ttttgatgat
     9061 caccgcagag gctgaacgcc agcgcatcga agaggtgatt tcggcaggtg tcaatggcct
     9121 gctggtcaag ccttacaacg ccggtagcct gaaagccagg ctggaacgca tattcaaccc
     9181 gcaaaagcgc gtgttgccaa agccattgcc accgcatgta gccagcaccc gggcggacga
     9241 tgggccgggc accggctcaa tgttgtcgcc gtcgcgcatt ctggtggttg acgattaccc
     9301 ggtcagtctg aagctgctgg aaaagctgtt tgccgacgaa tatctggtgc aaacggccac
     9361 cgacggtgcc acggcgctgg ccctgtgcca acacgagccg ctgccagacc tggtgctgat
     9421 ggatgtgttg atgcccggca tggatggttt tcaagtggtg cgaagcctgc gcgagcagcc
     9481 cgccaccgcc cagatcccgg tgatttttgt caccggtctg agcgatgacg aggcccgcat
     9541 caagggcatg gagcttggcg cagtggattt cgtgtcgaaa accaacgacc ccaaggcctt
     9601 gcgtgccaaa gtccgcaatt tcatccattt tgtcgatatt cggcgcaaac tgcagcttga
     9661 ctacgatgcc atgctggatg ccgcgcgctc gcgtgaagaa gccgaaaata tcactcgcca
     9721 tgacctcaag ggctcgctgg ccggcattgt cggcatggtt caaagcctgg ctgatgacgg
     9781 cgacatgcac ccccggcata cagcccagtt gcgactggtg cagcagaccg ccgaacaggt
     9841 catgaacatg gtcaacctgt cgggtgagct gtacaagatc gaaatgggcc gcttcaagct
     9901 caagaccacg gcggtggaca ttggcgagat attgcaccgt attgtggttt tatcgcgtgc
     9961 cggttttgcc gacaagcgcc tgaccatctc agtggacacc gacagcccgg tcggcgccga
    10021 actgcccaag gcgcaagccg acgccatgct gtgctattcg ctttttcaga acctggtcaa
    10081 gaacgcctgc gaggcggcac cgccggacag ccgggttgaa gtgttgttgc gcgacgagtc
    10141 gccgctgcgc attttggtca gcaacatggg ggccgtgccg cctgaactgc gcgaacgctt
    10201 ttttgacaag tttgccacca gcggcaaagc tggcggtagc ggtattggca cctattcggc
    10261 gcaattgctg gcgcacgccc aaaacggcag catcagcatg gtgaccgacg atgccaacaa
    10321 ttgcaccacg ctgaccgtga ccctgccgcg gcacgcgttt gacgcggccc attccaacgc
    10381 ctgacggctt tttatcaaga ggacagctat gggaaaccga ctgacaaaaa tcgccacccg
    10441 aaccggtgac aacggtagca ccggcctggg tgacaacacc cgggtttcta aaaacagcct
    10501 gcgtgtgcat gccatgggcg acgttgacga actcaactca aacattggcg tgctgttgtg
    10561 cgaggacatg cccgctgatc tgcgcagcct gctggtggaa attcagcacc agcttttcaa
    10621 cttgggcggc gagttgtcga ttccggggtt tgaactgctc aaggctgagg cagtgctggc
    10681 gctggaccaa gcgctgcttg aatacaacgc ccatctgcca attttgcagg aatttatcct
    10741 gcctgcaggc acccgggctg catcgctggc ccatgtctgc cgcaccgtgg cacgccgggc
    10801 cgagcgtgcg gtggtcgcgc tgggcgatca ggatgccatc aaggacaccc cccgccacta
    10861 ccttaatcgc ctgtcagatc tgatgttcgt gctggcccgg gtgctcaacc gcttccgcag
    10921 cgacggcagc acaggcgacg atgtctactg gcgcagtgag cgcctggcgc aaactgccga
    10981 caactgaccc agcggctggc gtgggcggtc tcggcgagtc tgcgctgctt tggtttgcgt
    11041 caggcctgtt tggccttgcg caaaacagcg taaaccgcat ccttgagatg caatttttcc
    11101 tttttcagtt tttcaatttc gaggtgggtg gcgggttcaa cgtgtgactc catgcgcttg
    11161 atcttggcat caagcgcatc gtgctgatca aatatctgcg cgaaatgcgc atcttcagcc
    11221 ttgatctgga caatttgttc gcggtattca gaaaacatga aatctccggt ttgagttggc
    11281 tattgggttt gaattttcga gacaaaactg acagacccgc attatccatc cggtcagccc
    11341 cgaaattgcg gtcgttgaaa gatgcaattt tgctattttt tgtatagctt gcagcgcaat
    11401 cagcataagg gatagaagcc aaaaatatca aaaatctgca agctgatcca tgcgtcacaa
    11461 gccgataagc cgggcttttt gatgcggctg ccagtggcgc agccgctctc atcacgccgg
    11521 gctgatttat catggatcat gaaattgtta cgctccagtt tgatcaaggc cggtgccgtc
    11581 tgggtgctgg cttgtctggc cctgccgggc tgttctgaca agacggcaac ggccggtgac
    11641 cctgcggttg cgggtgcctc tgctgccgcc agtgtgcctg ccattgccgc gtcaagcgca
    11701 gcgcccccgg tgtcggtcac catcgccacg gccaccatgc gtgatatggc ggtgaacttg
    11761 cgcgccaccg gcacggttgt gccgctgaca agcgttgatg tcaggtcgca agtgaccagc
    11821 acggtgcgcc aggtgcattt caaggagggt cagtttgtca aagccggcca attgctcttc
    11881 acgcttgatg tgcgcgcaga cgaagctggc ctggcccgtg cccgcgcgca gttggcgcgc
    11941 gaccaggtca gcctggctga tgccagacgt cagcatgagc gtgccagcca attgctggcg
    12001 caaaactttg tttcgcaggg tgcagtcgat accgctcaag cccaggtgga tggctggcgc
    12061 gcgacggtgg cggctgatga ggcgggtgtc gctgccagcc aggtggtcct gtcatacggt
    12121 cgcatcaccg ctccaaatgc cgggcgcgcc ggtgccgtca atgtgtttgc cggcagcgcc
    12181 gttcaggcca accagacgcc gctggtcacc atcacccagt tggacccgat cagtgtcagt
    12241 ttcagcattg cacagcgcga tttgtccagc gctctggcag cactgggaga cggcggggcg
    12301 gtggtgcagg cgactttggc cgacggcggc ggcggcttca gggggcgctt gcagttcatc
    12361 gacaacctgg tcgatgccag ttccggcacg atcaaggcca aggcggtatt tgacaacccg
    12421 gcagccaggc tgtggccagg tgcctttgtc gaggtgtcac aaaccgtctc gacgctagcc
    12481 ggtgcagtgg tgttgccaca ggcttgcatc attcagagca ttcgcggtgc cattgtgtat
    12541 gtgattgccg atggcgtggc cacggtgcgc ccggtcaagc tgctgtatgc gcagggcggt
    12601 gaggcggcag tgaccggggt caaagccggt gaagcggtgg cgcttgatgg ccgccaaaac
    12661 ctgcgcccca acgcgctggt ggtggtgcgt gcgccggcca gcgcggcagc gggcgacgca
    12721 tcgggcaaag ggccgggcag cgggtccggg ccgggtacga agaagtcgag ccagccatga
    12781 atttgtcgga agtctttatt cgccgtccgg tcatgacggt gctgctgaac ctggccatcg
    12841 tactggccgg ggtgattggc ttcaggagca ttccgattgc cgccttgcca agctacgaca
    12901 cggcggtgat cagcgtgtcg gcggcgttgc caggtgccag cccggagacc atggccatct
    12961 ccgtggcact gcccctggag aagcagtttc agaccgtgtc tggactcaag accatcagtt
    13021 ctaccagcac actgggcaac acatcgctga ccctggaatt tgaagagggt cgcgatattg
    13081 atgctgctgc gctggatgtg caggcggcgc tgttacgagc ccagcgcgcc ttgccagatg
    13141 acatgagcaa cccgccgtcc taccgcaagg tcaacccggc tgatgcgcca gtgctgctga
    13201 tctcgttgcg ctcgccatcg ctcgccccag ccgatttgca ggactatgcc gagcatctga
    13261 tttcgccgac gcttgcgacc atcagcgggg tggcacaggt gaacatattt ggtgccaagc
    13321 gctatgcggt gcgggtacgg gtgcagcccg atgccctggt ggcacgcaac atcggccttg
    13381 acgaagtcag ttcggcattg cgtgccgcca acgtcaatac gccggttggc acactcgacg
    13441 gtccgacgca gacgctggtg ctgcaggcca acaagcagtt gcgcaacgcc gctgaatttg
    13501 ccgagctgat catcagcacg cgtggcggca acccggtacg gctcagggat gtggcgcgag
    13561 ttgaagacag tctggaaagc ctcaagagct gggccacctt caatggtgaa ccaaccacca
    13621 cactggccat attgcgccag cccggcgcca atacggtgca ggtggttgat gcgattcggg
    13681 ctgccttgcc cggtttgcag gcgcaactgc ccaagtcggt gtcactgaca cccatcaacg
    13741 accggtcgct gtcggtgcgc gaagccttgc acgatgtgac gttgaccctg ctgggcacga
    13801 ttgcgctggt ggtgctggtg atcttcctgt ttttgcgccg ctttgttgcc actgtgattc
    13861 caatgctgtc gttgccggtg tcgctgattg gcgcggtggc gctgctgtgg ggcctgtcat
    13921 acagcctgga caacatttca ctgctgggcc tgactttggc ggttggcctg gtggtcgatg
    13981 acgcgatcgt ggtgctggag aacatcatcc gccatgtcga gcgtggtgaa aacccgtttc
    14041 aggcggcgct gcgcggtgca cgtgaggtgg gcttcaccat cctgtcgatc tcggtatcgc
    14101 tgattgcggt cttcatcccg atatttttca tgccaggggt cattggcctg ctgtttcacg
    14161 agtttgccgt ggtggtgggg ttgtcgattg tggtgtcagc ttttgtgtcg ctgacattgg
    14221 tgcccatgct ggccagccgc tttctgactg acgaggcgca caagaagccg ccgggcgcgc
    14281 tggtgcgggt gttcgagcac gggtttgact ggatgctggc tggctacacc cgctggctgg
    14341 accgggcgct ggcacaccgg cggctgattc tggctctggc cagcgccacg atcgtggcga
    14401 cggtctggct ggcgatggtg attcccaagg ggttttttcc cgaagaagac ctcgggcaga
    14461 tgtctgtcac caccgaggcc ggtgaagaca cttcgtttac cgagatggtt cggctgcaag
    14521 agcgcgccgc cgagatcatc cgcgctgacc ctgcagtggc agcgttgaac tcgttcaatg
    14581 gcggcagcgg tgcacaaaac accgggcgca tgttcatcac actcaaaccc cgcgccgaac
    14641 gtccgcccat gaacaaagta gtcgaagggc tgcgcaaaaa gctcaacaac ctgcctggta
    14701 tcagactgtt catgcggccg gtacaaaacc tgcagttggg tggccgaccc agcaaagccc
    14761 agtttcaata cattttgcaa agtgtgcgcg ccgatgaact caatgactgg gccaacaagt
    14821 tgcaggagcg cctgcgcgtc gatccgctgt ttcgggatgt caccagcgac gcgcagttgc
    14881 gcggcctgca agcccagctc aagattgacc gtgatcgcgc caatgcgcta ggtgtctcga
    14941 ttgagggtat ccgtaccgca ctttatagcg cctttggcga gcgccaggtg tccaccattt
    15001 acctgcccac cgatagctac caggtgatca tggaggtagc ccccgaggcc aggcaggatg
    15061 agagcgccat cgccggaatt tacgtgcgtg catcgaccgg tacgctggtg ccgttgtcga
    15121 gctttaccac agtcgagcgc agcgttggcc ccacctccat caaccatgtc gggcaactgc
    15181 aggcggtcac cgtgtcgttc aacctggcac caggtgctgc gctgggcgat gccacgacca
    15241 agatcgacgc agcgcgcgag gcgattcaaa tgccggcttc catcatcagc acctatggcg
    15301 gcgacgcggc ggtgttcaaa agttcgcagg ccagccagat ggtgttgatt ctggctgcgc
    15361 tgctggtgat ctacgtgttg ctgggcgtgc tgtatgagag ctttatccac cccatcacga
    15421 tcctgatggg gctgccttca gcggcagtcg gggcgctggc cacgctgatg ctgttcgggc
    15481 aggatttgac cttgattgcc accattggca ttgtgctgct gatcgggatt gtgaaaaaaa
    15541 acgccatcat gatgattgac tttgcgctcg atgcccagcg ccatcagggc atggccccgg
    15601 cccaggccat ccgcgaggcc tgcattttgc ggtttcgccc gatcatgatg accaccatgg
    15661 cggcgctgat gggtgccttg ccgattgcgt tgggactggg tgccgggtca gagctgcgtc
    15721 aaccgctcgg cctcgcagtg gtcgggggac tggtgttttc gcaggccatc acgctgttta
    15781 tcacgccagt gatttatctg gcgctggacc ggttcagcgg tcatggtccg gtgctcaccc
    15841 ctgaggtacc ggtgggtcag gatgatgcgg cttgaaggcg aattggggca aggatgggcc
    15901 atggaatgat tgcgctggcc cgctacaagt gactgttgtc acaaagtcga atagtcccat
    15961 gggtgtgatt tgcggccctt gagtcatctg gtggagacgc gtttggataa acaacaatac
    16021 caagcccgcc gcaccgcagc gtcaccatga cgactgcgac catcttgatg ctggaaaaat
    16081 gggagtcagt ctgttggcca ttctttcaat gactcttttg accatgctgg tggtgctggt
    16141 gactatgctc accatcagaa tcatccagtc catgctgcgc ggccaaactt gcgtgccggg
    16201 ttgtgccagc aaggatcagc gtggtgcagg ttggcaggct ccgggttttg gatgcgcact
    16261 aaaagcgcta tcaattatga agcatcgggc gcaatgccca tccgaggtgg agataaaaat
    16321 gagggcgtga gtgaggattc tgaccgagtg cattttttgc tgctgcaatg caccattttc
    16381 gcccgcaaac aaatagaata gttgcaatca tgtcagcccg acatgtgtat tttgtcgatg
    16441 ctaacctttt tgggagcgtg aaaatggcag agtttgaaga atccagcttc ggtaagcgaa
    16501 gcgcccgcga tccacggttt gtcagtcaac tgacgcgcaa cacattcgcc ttgattctgg
    16561 cgggcggtcg cggcagtcga ttgcatgaac ttaccaactg gcggtcaaag cctgcggtgc
    16621 cgtttggcgg caaattccgc atcattgact tcacgctttc aaattgtgtg aattccggcg
    16681 tacgccgggt cggcgttgcc acccagtaca aggcgcaaag cctgatccgg catctgcaat
    16741 tgggttggag ctttctggac ggccgacttg gcgagttcat tgaaatcatg ccggcccagc
    16801 agcagatgga cgactcccac tggtaccagg gcactgccga tgcagtgttt caaaacttac
    16861 gcgaactgcg catcgccaac cccgaatatg tgttgatcct gtcaggcgac cacatctacc
    16921 gcatggacta tggtcgcatt ttggcggccc acgtggaaca taaggccgac ctgactgtag
    16981 cctgcatcga agtgcccctg aaaggcgcct ccgagtttgg cgtgatgacg gtcaatgaag
    17041 aagggcgtat tgttgacttt gccgaaaagc caatcaatcc acagccgatg cccggcaatc
    17101 cggacatggc actggccagc atgggcattt atgtgtttaa cgcacgcttt ttgtttgagc
    17161 aattgttgcg ggatgcagat gaccccaaat ccagccatga ttttggcaag gacatcatcc
    17221 cgcactcagt caagcggtat cggacctatg cccacagctt cgccgacagt tgcgtttacg
    17281 aaccaggcaa ggccatttac tggcgcgatg tcggtaccct tgatgcctat tgggcggcca
    17341 atatggactt ggtccatgtc acccctgatc tgactttgta tgacgatacc tggccaatct
    17401 ggacctggca accgcaaagt ccgccagcca agttcgtgtt tgatgacgat acccggcgcg
    17461 gccaggcggt ggactcgatg gtgtcgggcg gctgcatcat cagcggcgcg accgttcgaa
    17521 ggtccatgct gttctcgtgt gtcaatgtgc acagctattc cctggttgag gaatcgatcg
    17581 tgctgcccaa tgtgtcaatt gggcgcaact gcgtcatcaa gaagtgcatc ctggacaaga
    17641 attgcatcat tccagacggt acccagattg gcgtgaacct cgaggatgac aaaaagcgct
    17701 atcacgttac cgaatccggc atcacactgg tcaccgaagc gatgctgggg caaggcaagg
    17761 tcaacatccg ctagtccaga tgggtcggtt gcatcgcaat gaccctcccc acctgagatc
    17821 ggctaaaggt ccagcaccag catgtcgatc acccgcaagc tcaagccggc attgggccgc
    17881 aactggtagc taccgcactg cgggcaggcc aggccgcgtt ctgccaatgg cacggtctgg
    17941 ctgcagttca tgcaccaggc ctgtccttct ggctcttcaa acacaagacg cgcaccttcc
    18001 agcagggtgc ctggcgcaat cgcctcaagt gagaacttca gcgcctgcac ttcgatattg
    18061 gccaatttac cgacttcaag cgtgagtacc gtgacacgct tgaagccctc acgcttggcg
    18121 ctgtcttcga ccagttgcaa aatgccacca gccaggctgg cttcatgcac tgaccggctc
    18181 ctgcaggatg gcggactcaa gccatgcctt ggcccaaatc aaggcagcgg ccagattggt
    18241 ctgagccgct ggactcatgg cctggcccag ttcaaatgac tcgccgcgaa ttgccagcat
    18301 ggtggtggcg ggggccacct gtccatgcac atcagaaaaa acttgcagca gggctgaggg
    18361 tgtcagcgcg tggctggtgt aactgtgatc aggatagggc tgcggctggc gtacttcaaa
    18421 cggtgcggcg cagtccaggc atgcatctat cagcaatacc tgtcgccgcc ccaacaaatc
    18481 cagcgtgtgt tcaacctgca actgatagtc gtccagaaac tcaatgctgt ttgacagcgc
    18541 atcacgaaac tgcccttgca gtgccgccac acacagtggt cccagggcat catcaccacg
    18601 gctgaggttg ccccagccaa gcaccagcac gggcaacggc gttttatggg cttggagcat
    18661 caatccgaag tgcttgaaat gctatgctat acatagctgg tggcgcttat ctgatatggg
    18721 ctagagtgca ttttcattga aaaaattacc agtcggacgg gataaaccac cggcagatga
    18781 cttttgcacc cggtccagca ccgagccatc gcttgacagc aaaacgacat ccagcggcat
    18841 ttgtccaagc gcgtgggtgg cgcaggacag gcagggatcg taggcacgaa tggccacctc
    18901 aatgtggttg agcaaacctt cggtgatctc atggccgttg aggtagtcac gcgcgaccga
    18961 ccggaccgcc tcgttcatgg cctggttgtt gtgggttgtg gagacgatca ggttgcagcg
    19021 cgtcaccaga tcgtcatcac ccacgtcgta atcgtgaatc agcgtaccac gcggagcctc
    19081 aatgatgcca acaccgctac gcaggcgatt gccgctggtc agcagatcgc ctgaaagcag
    19141 cgccgggtcg tcgagcaact gaccgatcac ttccaccgaa tgcaacattt cgatcatgcg
    19201 agcccaatgg taggctagcg aggcatgaac caggccgcct ttgccccagg caacaaattc
    19261 ctgccgctcg gcctcggcaa gggggctcgg aataaagtcg cagttttgca cgcgtgccag
    19321 cgggccaacc cgataccagc ccaattcgcg ccccagactt ttcaagtgcg ggaacttcat
    19381 gtagctccag gagcggacct cttcctcgat caactccagg tagttctggt cactgacacc
    19441 gtcaaacagg atcatgccat tggcatcacg cacccgcagc acaccgtcat acagatccat
    19501 ggcgccatcg gagcgcacca atgacagcaa gttggaacgg aagctgccaa actggttgta
    19561 cagggcctca ctttgcgcat gcaactgctt ggcaagatcg accgaatcct gcgcccactg
    19621 cagcatcagg ggcaactcgt ttttcagtgc atcacgctcg gctgcagtca ccagtttgtt
    19681 gatgccgccg ggtactgcgc cggtaccgtg aacccgtttg cccgaagtga tgcgaatgat
    19741 ttcctgcccg aacttgcgca gcaaaatgcc cttttttgca acctccgggt tggcttgaac
    19801 caccccgaca atgttgcgct gtgccaccgg ggaatcaaag ccaaacaaca ggtcgggtga
    19861 tgacaggtgg aagaaatgca gtgcgtgaga ttgcagcatt tgcccatagt gcatcaggcg
    19921 gcgcaccgcc tcggcactgg cggtgatggg catggcaccg atgatccggt caaaggcctt
    19981 ggaagcggcc aggtggtggg acaccgggca aatgccgcac aggcgttgca ccatgaccgg
    20041 cacttcccag tacgggcgac cttcaatgaa cttttcgaag ccacggaatt caacgatgtg
    20101 caggcgcgcc tgctgtacct ggttgtcgtc atcaagcagc aaggtgacct tgccatgccc
    20161 ctcaacgcgt gagaccggat caatcgccac ccggcgcagg cccttcgggt tggcggcggt
    20221 ttcaagatca aaactcatgt ggactccttg atggttgggg gcagcaatgg tcacttcggg
    20281 tagctgcggt ctgtcttgca aggccgacat ccaacatccg acaagatgtt gaggacagag
    20341 cttgttcact tcgtgtaact gcggtctgtc ccgcaaggcc gacatccaac atccgacaag
    20401 atgttgagga cagagcttgc tcacttcgtg taactgcggt ctgacccgca aggccgactt
    20461 ccaacatccg acgaaatgtt gaggacagag cttgttcact tcgtgtaact gcggtctgtc
    20521 ccgcaaggtc attgttagtc atagtgaatc aggccgtggc caaggtgcgg ggtgcggccg
    20581 gccagcaggt cagtcagaaa ggtccagatc gcgtcacccg atggtggaca accgggcaga
    20641 aaatagtcga tatgcacgac ctcgtggatc gggtgcacat ggttgagcgg cagcggcagt
    20701 tccgggtcat tgggaatctg gttgccaatg ctgccgcccg cctgcctgca gtaaacatcg
    20761 cgcatcattt ttcccacatc acgctggttg cgctgagcag gcaagccgcc gttgatggcg
    20821 caggcaccca ctgccaccag aatcttgcat ttggcccgaa actcacgcaa aacatgaaca
    20881 ttctcggcat tgcaaaggcc accttcaatc aagaccaggt cgcaacggcc aattgtcttg
    20941 atgtcggtga gcggagagcg gtcaaattca acgagttcaa gcaaatccag caatcgctcg
    21001 tcaatgtcaa gaagcgacat gtggcagccg aaacaacctg ccagcgacac cgtggcgatc
    21061 ttcagcttgc gtgcggcagg tgctgggctg ccagccaagt cactgccgta ggtaaatgcg
    21121 ttcatggctt gttctcatca atttgtcctg acacaggaac cttgtcaaag cgtctctgac
    21181 caatcgggat gacaaagccc ttacgcttgt gcaggatggc accgaccggg cagacattgg
    21241 cagcgcgatc tgatggaagc aatgcgctgt caaccatttg gccgctgggg ctgttgatca
    21301 gtaagtgggt tttgatgccg taaccaccga tggcaaacac gtccttgcgg tccacttcat
    21361 ggctggcacg cacgcacaaa gagcacagga tacagcgatt gaggtcaagc agcatatccg
    21421 gatggctggc atcgacccgg cggtccgggt agaactcttc aaagtgaggg ccttccatgc
    21481 cgatgtcata agcagtggct tgcaatcgac agttaccact tttctcacac gaagggcaaa
    21541 aatggttgcc ttcgataaaa atcatctgca acaaagtctt gcgctgggcg ttgaggtcgt
    21601 cggtgttgct ttcgacttcc tgcccggatg ccgcctgcac cgtgcaagct gcccccatac
    21661 gcccgttgat acgcacagta cacagtctgc aagaaccctt ggcaccaaat tcaggatgcc
    21721 agcacaagtg cggtatgtag tagcccgctc gggtcgcggc ctgcaggata gattcccctg
    21781 gaccgaactc aatttcgtca ccatcgagcg agaaggtgcc cgggctcaga gcgtcaatgc
    21841 ggtcgtaata actcatgtca aggtctccag gtgggcacct atgtcgtcac gtccggtggc
    21901 cagacgcgcc tgcgacagct ctgcatccat atcaaagccg ggggcaaaat gcattgactt
    21961 cagatgctgc tcgtaagccg ggcggaactt ggcgatggtg tcgcgcaagg ggttgcaagc
    22021 cgctgcgcct aaaccgcaat gggttgagcc gtgcatcagt ttgtccagtt cgtaaagtac
    22081 ctggatatcg tcacttgaac cgtagccccg cttgagcttg tccatgcgcc gcatcaccag
    22141 ttcggtacca acacggcaag gggtacaaaa gccacagctc tcatgggcga aaaactggac
    22201 aaagttgttg gccacatcaa acatgttgcg cgagcggtca aacaccatga aggcacctgc
    22261 tgtgggcacg tcctcaaaac cgatgcgtcg gccaaattcc atggccgaca cgcacacccc
    22321 ggacggtccc ccgacctgca cggcttgcgt gtcccgggca ccacagtctt ccagaatgcg
    22381 tccgatgcgg gtgccaaatg ggtattcgta aatgccaggg cgatcacagt cacctgatac
    22441 cgagtggatc ttggtgccag ttgattgcgg ggtacccatg gcggcccacc aggcaccgga
    22501 atggatggca atgtgagtca cggcacaaaa agtttcgaca ttgttgaccg tggtgggctg
    22561 tcccaggtag ccacgttcaa ctggaaacgg cgggcgaatt cggggtgttc cacgcttgcc
    22621 ttcaagcgac tcgatcagtg cagactcttc gccgcacaca taagaaccag cacccacatg
    22681 gatatcgatg tcaaagtcaa aacctgcctg cccctgaatg gactggccca gcagcccttg
    22741 ttgccgacgc cgctgcaggg ttgccttgag cgaatccagc aaatagtggt attcgccacg
    22801 caggtaaacc aagccctggc gcgcaccgac gatcagcgca gcaatggtca tgccctcgaa
    22861 gacagtgtcg gcataactgg tcagcaacac cctgtccttg aaggtgccgg gctcgccctc
    22921 gtccgcattg cacaccacat agtgtgtttc agaccgggta tttcggcaga gctgccattt
    22981 ggttccggtg gcgtagcccg cgccgccacg accgcgcagc ttggagcgtt tcaggtcatc
    23041 gagagtggcc tgggcaccac gcgccagggc cgccgcaatc gcctgacccg gtgcaggttg
    23101 tgcccccagc ttgacattgg ccaggcgaat ctggtcatcc acccgtgacc attcggcagg
    23161 ccagagatgg gttggcacct gcgcttcgat caggtccgcc atatggtcaa tacgaccggg
    23221 ggtcagacgg gtgaccagtt ggtggtggtt gaccagcgcg gccggcccct ggtcgcacat
    23281 gccggtgcag gaagtggcgg cgacgctgac acggccatca gcgcgcatgg cgtggtgctc
    23341 gaccttgagc agatggcgca gatgacgccg catttcttcg ctgccctgca tgcggtcggt
    23401 gatgttgtcg ctaaacagca cgcggtactg tccgactggc tggctgtgga agaaacgata
    23461 aaaccccgca accccctcga catgcgccag cgacaggccc aaggcgctgg caacagcagt
    23521 caaagtgtct cttggcagcc aaagcaattg cgcctgaatt tcgcgcaata tttgcaccag
    23581 tcggtgcggt gcccgctggt gccgctccag cagactttcg agatcgagtg cgtttgggtt
    23641 catgtctcac accttatgtt tttgcaactg taagcacaga ataacggcaa gcgcagtaga
    23701 taaggtcttt tttttcaaaa aaagtgcgct ttttagttcc tttgcagcaa taaaccgcaa
    23761 acttctgcag cctgagcaaa atcaaacgtc gccatggctc gcgtcagttg cttgaactcg
    23821 tcaacgcttg tcagtgccga attcaactgc aactgttcga acgcgctcac tgcagccata
    23881 tccgaggacc ttagcagtac ctgaagttgg cgcagcatct cgtgtccatc gggtgccaag
    23941 tcggcatctg gcgttgctac atgctggtca atagtccctg gtgcgcaagt attggccact
    24001 tgtcccatca cgatctgggt tgccgcgact gccgcgctga agctgatact ctgtgcccgc
    24061 acgtccagcg gcgcagcatc tttgcttgct gctttggtct ttttgatgac taactcggct
    24121 tttcgcgcaa ccgccgccat gtaactggca cccactgtgg ctgacaggcc ctttaaagca
    24181 tgcaggattt tctcggcaga ctcccagtca gacttgttca aggcaatatc caattgctca
    24241 ggcaacccgc caatgtcggt caaatacgac tgcaacaccc gcacgtaaag cgctgaattg
    24301 cctcccaaac ggtcgagcgc accgaccgtg tctaccgcat caatacgtgg cagagcgggt
    24361 tccggaagtt ggtttggcat caaatggggc accatagacc cttcacctgt catcgattcc
    24421 gctgacgggc ggcgggtcaa ttgttgcaac aacgctacaa gatcgaccaa tttgaaaggc
    24481 ttgcctacat gcgcgttcat gccagcggcc agacaggcat cgcgatcact gtccatggcg
    24541 ttggcggtca tggcgatgat tggtaaatct gccaagcccg cattctggcg caacactcgg
    24601 gatgcgctgt aaccgtccat gacgggcatc tggatatcca tcaataccgc atcaaacggc
    24661 tttccgtctg cgatagcgcc aaataccgca tccacgccga cttggccgtt gtcagccaat
    24721 tcgaccaagg caccctcgtt ggtgagcatt tcctgcgcca cctgctggtt gaccatgttg
    24781 tcctcgacca ccagcaagtg catgcccagc aaaggctgtg gcgcggcgtt ggcagttgcc
    24841 ttggtctggg gatgtccgtt atcggccagt gcatcgacca ccgcgtcata cagcatcgaa
    24901 gccgtaaccg gcttgaccaa aaacccgttg agtcgggtct gctcttcgac actgcgctgc
    24961 gccagcatct cgcgtccatt ggcggtgatc atcacaacaa cgggtgagtg gctgttgcgg
    25021 ttttcctgtt gcattcggcc gatggtctcc cagccatcca tgcccggcat ttgccaatca
    25081 acaaatatag cctgatacgg atcttgcccc gaagacgcgc gctgatcaat cagccaaagc
    25141 gcatcagctc cgccagcggc ctgatctact tgccattcca gagactcagc catggaggca
    25201 agaacactgc gcgcagtcgg gttgtcgtcg accaccagca ctcgcaaacc ggacggcagg
    25261 gctggggaag ttgtcgcggc tttgggctct tctggtgcca ccggcaatgt gacggtgaag
    25321 gcaaaggtgc tgccgatgcc caattgactc tcaagcgtga gttccccgcc catcaatgcc
    25381 accagacgcc gactgatgga caagcccagg cctgtcccgc caaaacgtcg ggtggtcgat
    25441 gcttcagcct gcgaaaaccc atcaaaaata tgctgctggt tggccgcggc gataccaatg
    25501 ccggtgtctt ccacactgaa ttcaatgtgc gcgatatc
//
LOCUS       DAOSLL010000002        23352 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_2425_length_23352_cov_5.020045, whole genome shotgun sequence.
ACCESSION   DAOSLL010000002 DAOSLL010000000
VERSION     DAOSLL010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 23352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23352
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2425_length_23352_cov_5.020045"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="PLB25_00115"
     CDS             complement(<1..223)
                     /locus_tag="PLB25_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOE40032.1"
                     /translation="MHINIVPNRGSAPTVLLRESYREGSKVCKRTLANLSALSAAQVQ
                     MIRATLRGDVLQPIDQTFEITASPAHGHVQ"
     gene            complement(542..1069)
                     /locus_tag="PLB25_00120"
     CDS             complement(542..1069)
                     /locus_tag="PLB25_00120"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE40033.1"
                     /translation="MDDAKSKVHSRKELSPLGDRQAWGGTTTIGFEWQKCERLEMNNA
                     TRKGQIGSVLVVDDDDISCDIVCAMLGDLGVTEVHCAGNGQAGLRTLAELANTPDVLI
                     CDLFMPDMDGIEFMAELARQRYPGQVILISGGNVEMLVVAKELALAGGIQLLGCLTKP
                     LYCEMLQSVLAIKAR"
     gene            complement(1109..5893)
                     /locus_tag="PLB25_00125"
     CDS             complement(1109..5893)
                     /locus_tag="PLB25_00125"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE40034.1"
                     /translation="MRWHLSGFGPVRLALLVWLVSMAATGLLWQQASQNSENELRADF
                     LREVASAQRSIETELAANASVLKSFTGLFNASSEVTRQDFRTFFESLSLDQGVHNFAA
                     VTYVENVPAGKLDQHLAGVRRDGQADYQIHPAEPREAYAPVVFIEPFSGDNRRVPGFD
                     TFSVLPARAAMQRARDSGALTLSGKLTLKQDEGKGVPGFVIYQPLYRRGTAPDTPALR
                     QTHLLGWVNVPFRAADLLKHALPQNFENIGLEVFDGNSQSLDTLLFDSDATPTFELPV
                     AAGFESVRPLVFGGRTWTLVFRALPGFGSQAVAQGPQQLLLAGLLLSALLGFVAALIG
                     RARQRRDDLAQKMVALAAAQGREAERAASAEMLLQNAWAMNEAQRIGQIGTYVTDVQT
                     GLWQGSPVLDNIFGIDASFAKTVDNWSRLVAPESQAELLGYYHRVVAAKGKFKEDYKI
                     IRPADGKTRWVSALGELTFDASGKPLLLRGTIQDITERKLAEMDLRDSEFAARLALDS
                     ANALTKKLEKAQAELAGREEIYRSIVTQAVDGMVMIDTSTLAYVEFNDAACRDLGYSR
                     EEFARLTLADVQGELDAEATRTGVAGMVAAKTSSFETLHRHKDGSLRNAYVRSSIVWR
                     AGLPYLVGIVSDITERKASEYELQNYRDKLEQMVLAKTASLQQAVGAFQASEARYRRL
                     IDNSHDIIYTINTSCLLTFVSPSWTLLMGHSVEKILGKPFADIIHPDDLADSRAAIQR
                     VMATGASLQGLEHRTQHQDGSWHWFNTNGTALRDESGKVVGFEGTAGDITVRKQIEVA
                     LRTSRQRYLSLIENLSDVLLTLTPAGIIDYVSPQWTVEFGHAVNEVLNQPFERFVHPD
                     HQAGCLANIGQVFASGSQQNGVEYQALCKDGSYLWCSAKSSLLKDASSGAVTLIALVR
                     NISQSKADQQALQWSVSLLNATIESAAHGVLAVDSGGQVTLWNQRFAELWRMPPELLN
                     APDKRHLRAFAAAQMGQPEQFIARVNAIYDQPQVASNEILELADGRSFRQISRPQKIG
                     DEVIGRVWSFDDITDLKRAEAAATTASRAKSEFLANMSHEIRTPMNGVVGMVDILQQT
                     GLTADQQRMLSTIYHSSLALLHILNDILDYSKIESGKLAVEHIPVNLRDVAHEVHQLL
                     RSTAQAKSLDLSVWVAPELPDWIFSDPTRLRQMLLNLIGNAIKFTRSTPDKPGRVQLR
                     VTVSTQPDGQPRLQLRLTDNGIGMSDELVARLFQPLTQADASTSRQFGGTGLGLSISQ
                     RLASLMGGQISVSSKLGQGSDFTLTLPLQEAPAALVHELMSDRRALPRLTKTPGTAPA
                     VAGTGPLILLAEDNETNRDVLYQQLKLLGCSAEVAEDGRVALEKWRSGQFALLLTDCH
                     MPQMDGFALAGAIRAAEGPGQHLPIIAVTANVMQGEVQRCLNSGMDDYLSKPLRLQEL
                     AAMLAKWLPQAGQPETRPALPTADRRSSPVGEPLPNPACPVWDADTLSKMIGDNPDMH
                     QSLLRKFLQNAQQQVKQIGIAALAHDTRQIADIAHKLKSSARTVGALALGELCEEIET
                     TGRADQPALCLKLAAALPDALAGAAEKIGVTLHLVDQTHSPG"
     gene            complement(5897..6298)
                     /locus_tag="PLB25_00130"
     CDS             complement(5897..6298)
                     /locus_tag="PLB25_00130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE40035.1"
                     /translation="MTEQDIKDFSYLIVDDDDFSLEIMTNVLICLGVNRIHTAQDAAL
                     AYRLAQQYRPDFVLLDIYMPGLDGWALLDQLRQLLPKIVVIMVTGSILPADFTKSMDQ
                     RVDGYCIKPVSSSIMSKSLMRAWQRRQLAAS"
     gene            6760..7785
                     /locus_tag="PLB25_00135"
     CDS             6760..7785
                     /locus_tag="PLB25_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE40036.1"
                     /translation="MNARQRFTRRIFNIATSALLTGAALGASAQSPVTLLNVSYDPTR
                     ELYSEFNAAFAKHWKAKTGQDVIIKQSHGGSGKQARSIIDGLDADVATLALAGDTDAL
                     HDNGGWIPKDWQKRLPHNSSPYTSTIVLVVRQGNPKGIKDWDDLIKPGISVITPNPKT
                     SGGARWNYLAAWEFAKRKFGGQAQAKDFIAKLYANVPVLDTGARGSSITFAQRNQGDV
                     FISWENEAYLLEKEFGSKVDVVYPSLSILAEPAVTVVDKNVDKKGTRAVAEEYLKYLY
                     TEEGQDLAGKHFYRPAVSEKAKAKYGKQYPKLNLFTIEDAFGGWSKAAKDHFADGASF
                     DQIYTKK"
     gene            8258..9550
                     /locus_tag="PLB25_00140"
     CDS             8258..9550
                     /locus_tag="PLB25_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013957028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HOE40037.1"
                     /translation="MALTNTAFGDPRVLLRQMFDAAVAAAQPARCLGAHLPAPPKGRT
                     IVIGAGKASAAMARAFEDLWTARDSSPLEGLVVTRYGYQVACQHIEIVQAAHPVPDAA
                     SQLAARRIRERVSSLSADDLVVALVSGGGSSLLVEPSEGLTLADKQAVNAALLASGAT
                     ISEMNCVRRHLSGIKGGHLAAACHPARLLTLLISDVPGDSPMDIASGPTVGDSTTCAD
                     ALAVADRYQIKMTPAMCARLISGAGETVKPDDVRLQHISTRMVTAPQMALEAAAGVAR
                     AAGYTPHILGDSLEGESREVARVLGGLARQVVRHGQPFQRPCVLLSGGETTVTIKGKG
                     RGGRNVEFLLSLALTLAGTPGVYGLAGDTDGVDGSEEIAGALLTPDTLERAWARGMNP
                     RSFLDNNDAHTFFQRLGDSVITGPTLTNVNDFRAIMIV"
     gene            10430..11623
                     /locus_tag="PLB25_00145"
     CDS             10430..11623
                     /locus_tag="PLB25_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HOE40038.1"
                     /translation="MLIGIICFFYFLGLFFAPFPCWPTVFKLWRTLLFDRADATSKIR
                     QFTFLILVIVRAPFWTFFWYIDELLFPEYRDQLIDPVFIIGQPRCGTTLLHRTLAQDN
                     RTFFAVRHIEWRYPFISVQKTINLLGLNKWLEKVDYWPKSQAGQMASKMHKNRLSDWE
                     EDGIFFEENFLHHFFIYLRFPYPDLLPHLDAYATLRLEDQKRMLEMHHKVLKKVSFLR
                     GAQGRFYLSKEVTSHNKLPALAARYPSARFIVVTRESSKFMSSLMPLVRMSTWSKTGV
                     DPLTLPNWEDSMLTRMRSDSLNLVEICEKVIPLERQTRVSASDVMVNVVDSISIIYRE
                     LGLVASREFVNYLTELEVNQEDRERGYEYLSLSPKGFEQYDDFAKTNAQQFRKKIHEI
                     KGHRL"
     gene            complement(11871..12335)
                     /locus_tag="PLB25_00150"
     CDS             complement(11871..12335)
                     /locus_tag="PLB25_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40039.1"
                     /translation="MITLFDFCVPNLLFGIQINAASGAKSQTVLQPRQGKAAICRVDM
                     EMTLTDWPAQTHCMKLCRAQRRLWTVWQLCWARLLSHWGPIPCNFGQAKKRRNRKIIS
                     SHILISRSRLEFAVLDWAGCSAEVRAIGLIRHQEEPTFSVPLACGNFHLSRL"
     gene            12724..13332
                     /locus_tag="PLB25_00155"
     CDS             12724..13332
                     /locus_tag="PLB25_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40040.1"
                     /translation="MTATLALNAIDDTALARLEAEGRLLNPLHKALTGKPGRVGFRGE
                     LALRFAPKLADEARPPELFSAQAMAIANVGEQHIPFFAGYLLSFAYLKDVAEVLGDAL
                     SASGKYFLFCNNIDLSKKYQVAYQGVTFYVLPIDESTVYNELLELLYLEKNDLKKKDT
                     AGKLDAVADAALKFKGSFDAISYEEGLKLMGPVRNPNENRPV"
     gene            13333..14181
                     /locus_tag="PLB25_00160"
     CDS             13333..14181
                     /locus_tag="PLB25_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--dinitrogen-reductase
                     ADP-D-ribosyltransferase"
                     /protein_id="HOE40041.1"
                     /translation="MGEDDALDQPSRWYGTNLVGLPTQLLASTAFNSHPLPLTIAGTC
                     ETHPGLFTLLESSQTLTEAQDMFVHYLSIAFGLRKPEPHELSSMGKSEQRRWRSSWRK
                     LLQGWGMDSNGAAGAVLKGWVESRFGLLPSFHKAPLARFPSPAWITYLQEKASSRLNN
                     NNIYQQLDLLFEFCQWSLRRIRTQSHCAPAQHLRLWRGSNQCEEQVVAGRLQNRRCTV
                     RLNNIVSLSLSPEDASCFGDWVFEVQMPVSKVLVFPELLPGQVLQGEQEVLALGGNFE
                     VMARYA"
     gene            14174..15088
                     /gene="draG"
                     /locus_tag="PLB25_00165"
     CDS             14174..15088
                     /gene="draG"
                     /locus_tag="PLB25_00165"
                     /EC_number="3.2.2.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047407 - ADP-ribosyl-[dinitrogen
                     reductase] hydrolase activity [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosyl-[dinitrogen reductase] hydrolase"
                     /protein_id="HOE40042.1"
                     /translation="MPDLLDRALGAYLGFAIGDALGATVEFMLPREIAYQYGVHRDII
                     GGGWLKLACGQVTDDTTMSLALGEALLIGGSQALRHHADGDDTLVRAIGDAYVRWFKA
                     RPVDCGNTCRRGIVRYMREGSLQGSLNPGDGGNGALMRNLPAALATLGDEAAFERVSM
                     AQARLTHHHPLSDAATLGLGRLLHLLLAGSDHSACQLWAAQWTTDNPAFRFKPYRGYA
                     SAYVADTVQTVLHYLTLFDDFEEALVATVNQGEDADTTGALVGMLAGARCGAARLPLH
                     WLHRLQPDMVRTITEQTRSLLALAGYSG"
     gene            complement(15363..16694)
                     /gene="hslU"
                     /locus_tag="PLB25_00170"
     CDS             complement(15363..16694)
                     /gene="hslU"
                     /locus_tag="PLB25_00170"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HOE40043.1"
                     /translation="MSSLTPQQIVSELDKHIVGQQQAKRAVAIALRNRWRRQQVDADL
                     RGEITPKNILMIGPTGVGKTEIARRLARLADAPFIKVEATKFTEVGYVGKDVDAIIRD
                     LADIAVKQTRLTETQKVRARAEDVAEDRVLDILIPMPRQEFGVLEANEPESTARQNFR
                     KKLREGLLNDREVEVDLAHAAPQLEIMGPAGMEEMTEQLRGMFGQLNQNKKQTRKLKI
                     GEALKLLADEEAAKLVNEDDIKTQALQMLEQNGIVFIDEIDKVTSRSEGNGAEVSRQG
                     VQRDLLPLVEGTSVSTKYGTVKTDHILFIASGAFHLSKPSDLIPELQGRFPIRVELQS
                     LSVQDFVAILTQTTASLVKQYQALLATEQVTVEFSDKGITRLAQIAFDVNERTENIGA
                     RRLSTVMERLLDEVSFNATNLAGQTIMVDAAYVESRLAELSKNEDLSRYIL"
     gene            complement(16831..17430)
                     /gene="hslV"
                     /locus_tag="PLB25_00175"
     CDS             complement(16831..17430)
                     /gene="hslV"
                     /locus_tag="PLB25_00175"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HOE40044.1"
                     /translation="MDQYHGTTIISVRRNTPSGMQVAIGGDGQVTLGNIVVKGTARKV
                     RKLYHGKVLAGFAGATADAFTLFERFEAKLEKHQGHLVRAAIELTKDWRTDRVLRRLE
                     AMLAVADLEASLIITGNGDVLEPENGIVTIGSGGAYAQASAMALLNHTELSAQDIVRR
                     SLEIAGEICIYTNMNHTIETLEDIAGQTKSHVRSGAAGK"
     gene            complement(17446..19137)
                     /locus_tag="PLB25_00180"
     CDS             complement(17446..19137)
                     /locus_tag="PLB25_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOE40045.1"
                     /translation="MATRGIGTGFLSKVAKFVLNPTVDWVDLDKPPAVSEEVNSKHAL
                     KQMIERKQYNDAVRKREFDKLRKLRRSAVPDTTSATLPPTSDQDSWGYAVYQERANTL
                     KKIDEIEAQMSKQWWKGRDGPASGKAAGRGLASPVAQPGNPAQEPMGIDSAFATTMPA
                     DLIESENGENSQQAPPVSQLPVPAVTAQTVTGVLTTPAFICADAQQNQVDPRLEEAAI
                     RFANSDDGGAESVLLMALQTPAAPTELVRAWASALLDLYRATGQRASFDQIASEYSLR
                     FACPVPTWVAGLQSPGSDTANATPVQLSQTDRAGYTCWSCPAELDAADVAQLQSLVKS
                     SLAPCWLDWSALKTITPQAAQPLAVLLATWCTLPLVLHLEGTESLDHLLRLHTPMGDT
                     HVAQFWWQLRLDLMRILRLHDEFELLALDFCVTYEKSPPTWQAARCQHIEGPGATAQA
                     ALSRPAATALLPAMHQDTASPNQALALTGEVLGDASQVLQALQTAFAANNTLVVYCAD
                     LIRVDFSAGGSILNWLANAQATGGHIELRDVHQLIAVFFNLIGINEHAHIIVRTN"
     gene            complement(19170..20081)
                     /gene="dksA"
                     /locus_tag="PLB25_00185"
     CDS             complement(19170..20081)
                     /gene="dksA"
                     /locus_tag="PLB25_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HOE40046.1"
                     /translation="MTVKVVNPEAISTQSGGVAKSLKSEVRPAARSAVNLATAAKTPN
                     VVTAKSAKPASPMSKKKPEEVTNPTQVEPVPHAAKVPRVTPAAVVKPVSGSPQLTPLG
                     VPVRSGRPSSRLAQLTVPSMAQSVASTAAKSSFTQISAVAPITPVLASAVKKDPALAD
                     NWKTKPVADLKDAEVIAMPDDAYMNDTQMAFFRMKLVELKNGILNNAGETTEHLREDT
                     VVVPDPADRATIEEEHALELRTRDRERKLLKKIEQSINRIDAGDYGYCDETGEPIGVG
                     RLLARPTATLSLEAQQRRELKQKMFGD"
     gene            complement(20278..21321)
                     /locus_tag="PLB25_00190"
     CDS             complement(20278..21321)
                     /locus_tag="PLB25_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOE40047.1"
                     /translation="MSLIPATILTGFLGSGKTTLLKRVLSEAHGQKIAIIENEFGEEN
                     IDNDILLTDSKEQIIQMSNGCICCTIREDLREALQLLAAKKRQGLLDFDRVVIETTGL
                     ADPGPVAQTFFMDDEIAESYLLDSILTLVDAKHAQKQLDDRQEARRQVGFADQIFISK
                     TDLVSPDETRDLQHRLTHMNPRAPQKAVHFGEVALSEVFDLRGFNLNTKLDIDPDFLK
                     EEVHEHAHEQHDHGEHCDHPSHQGGHHHLHDDDVKSFVFRSDRAFDPARLEDFLGAVV
                     NIYGPRMLRYKGVLYMQGTARKVIFQGVHQLMGSDLGPAWGPDEKPCSKMVFIGIDLP
                     SDIFLQGLEKSLV"
     gene            complement(21373..22554)
                     /locus_tag="PLB25_00195"
     CDS             complement(21373..22554)
                     /locus_tag="PLB25_00195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HOE40048.1"
                     /translation="MKTIRLKPGKERSLLRRHPWLFESAIADGSAEAGETVRVESSDG
                     QFLAWAAFSPAAKIRARVWSFDQHQRLDASFFIAACACSIRARARFDIKSDGLRLLHG
                     EADGLPGLIVDRYGDTLVAQFTSAGVERWKDVIAEALLATTGLTKLYERSDASSRTLE
                     GLPEACGWLRSAGDVELRLQEHNWQLAVNIADGHKTGFYLDQRDSRQRFAEYARRLRF
                     ERVLNCYCYTGGFTVAALEGGAAHVTSIDSSGPALTKAAANVALNGFAADRVSFMDAD
                     VNASLRQFIEQGRSFDAIVLDPPKFAPTVAHAERAARAYKDINRLAFKLLEPGGVLFT
                     YSCSGGISVDLFHKIVASAGCEAGVDGFILERMGAAPDHPMTINFPEGEYLKGLVVMR
                     K"
     gene            complement(22603..22914)
                     /locus_tag="PLB25_00200"
     CDS             complement(22603..22914)
                     /locus_tag="PLB25_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40049.1"
                     /translation="MQKQISVVLDNNDEFTFHVHDGVPLAHDAARAWLDQQFVALECE
                     PLRASGKVLTADKILVVTQAAGPTLFGDAKWAAEYVAAVSAALGKPMIRVDVPAMAIT
                     Y"
     gene            complement(22945..>23352)
                     /locus_tag="PLB25_00205"
     CDS             complement(22945..>23352)
                     /locus_tag="PLB25_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOE40050.1"
                     /translation="LRDQMPKGHAPQAPALAAATPGSVTDGVAAGTAAEPAALFIGQR
                     GTRLTAQSIWQRLKSRSLQAGLALPVHPHMLRHSFASHLLQSSSDLRGVQELLGHANI
                     STTQIYTRLDFGHLAKAYDAAHPRAHLKSKGSK"
BASE COUNT         4861 a         6669 c         6715 g         5107 t
ORIGIN      
        1 cttgcacatg accgtgggca ggagaggctg tgatctcaaa ggtttgatca attggttgca
       61 acacatcgcc acgcaaggtg gcccgaatca tctgcacttg tgcggcagac aaagccgaca
      121 agttggccag ggtgcgcttg caaaccttgg agccttcacg gtaggactcg cgcagcaaaa
      181 cggtgggggc ggagccacga tttgggacga tatttatgtg catgtctaca ttgtaaaaca
      241 taataacaat aataataaaa ttaatgcatg tctacatact tgcactcaaa aacgacacta
      301 acgtgttgat ttcaatagga atccaaccaa atccggatgg aacttcaccc taagccatcg
      361 atgtcgttgt tgtctcgcct ggccattgcg tctgcaatgg ctgcggccaa ggtgtccatt
      421 ggctggcaaa tccggcgcac actggcctcg gcgggggcga ctctggctat tcgatccatc
      481 agtccggcag gcacctggtc acgaatgatt cagcaatgga atcagcccgg ttttggtttg
      541 cttacctggc ctttatcgcc agcaccgact gcagcatctc gcagtacagg ggcttggtga
      601 ggcaacccag cagttgaatg ccgccagcca gcgccagctc tttggccacc accagcattt
      661 cgacattgcc accactgatc aggatcacct ggcccggata gcgctgacgg gcaagttcgg
      721 ccatgaactc aatgccgtcc atgtcgggca tgaacagatc acaaatcaac acgtcaggcg
      781 tgttcgccag ttcggctaac gtgcgcaagc cagcctggcc gttgccagca caatgaacct
      841 cggtaacccc gaggtcgccg agcatcgcgc acacgatgtc gcagctaata tcatcgtcat
      901 caaccaccag cacggaaccg atctggccct tgcgcgtcgc gttattcatt tcaagccttt
      961 cacacttttg ccattcaaat ccgattgttg ttgttccgcc ccacgcctgt ctatccccca
     1021 aaggggaaag ctcctttcga ctatggactt tggatttggc atcatccatc gtctgcgtgg
     1081 caatgagcta gatgccacga aaacaaattc accccgggct gtgagtctga tcaacaaggt
     1141 gcagtgtgac accgatcttt tcagccgcac ccgccagtgc gtcaggcaat gctgcggcca
     1201 gcttcaaaca cagcgctggc tggtcagcgc gtccggtggt ttctatttct tcacacagtt
     1261 cgcccaacgc cagcgcgcca acggtgcgtg cggatgattt gagcttgtgt gcaatatcag
     1321 cgatttgccg ggtgtcgtgt gccagcgcag cgatgccaat ctgtttcacc tgctgctggg
     1381 cgttttgcaa gaactttcgc agcaaagact gatgcatgtc cgggttgtca ccaatcattt
     1441 tgctcagcgt gtcagcgtcc cagaccgggc aggccgggtt tggcaatggc tcaccgacgg
     1501 ggctgctgcg ccggtctgct gtcggcaacg ccgggcgggt ctctggttga ccggcctgcg
     1561 gcagccattt ggccagcatc gctgcgagtt cctgcaggcg cagcggcttg gacaaatagt
     1621 cgtccatacc gctgttgagg cagcgctgta cttcaccctg catgacattg gcggtgaccg
     1681 caatgatggg cagatgttgg cccggacctt cggccgcgcg aatggcgcca gccaaggcaa
     1741 agccgtccat ctgcggcatg tggcagtcgg tcagcaacaa ggcaaactgg ccagaccgcc
     1801 atttttccag cgccacccgt ccatcttcag ccacttcagc gctacagccc agcagtttta
     1861 gctgttgata gagcacgtcg cggttggttt cgttgtcctc ggctagcaaa atcagtggcc
     1921 cggtgccagc aactgctggt gcagtaccgg gggtcttggt gagtctgggg agcgcacgcc
     1981 gatcagacat cagctcatgc accagagcgg cgggcgcttc ttgcagcggc agcgtcaggg
     2041 tgaagtccga gccttgccca agtttgctgc tgacactgat ctgcccgccc atcaggctgg
     2101 ccaggcgctg gctgatcgac aggccaagtc cggtgccgcc aaactggcgc gaggtgctgg
     2161 catcggcttg ggtcaatggc tggaacaaac gcgctaccaa ctcgtcactc atgccaatgc
     2221 cgttgtcagt caggcgcagt tgcaggcgcg gttgtccatc gggctgggtc gatactgtca
     2281 cccgcaattg cacccggccg ggcttgtcag gcgtgctgcg ggtgaacttg atggcattgc
     2341 cgatcaagtt gagcagcatc tggcgcaagc gggtcgggtc agaaaaaatc cagtcgggca
     2401 gttctggcgc gacccaaacc gacagatcaa gcgacttggc ctgtgccgtg ctgcgcagca
     2461 attggtgcac ttcatgcgcc acgtcacgca ggttcaccgg aatgtgttca acggcaagct
     2521 tgccagactc gatcttggag tagtccagaa tgtcgttgag gatatgcaac agcgccagcg
     2581 aagaatggta aatggtgctc agcatgcgct gttgatcggc ggtgagcccg gtttgctgca
     2641 agatatcgac catgccgacc accccgttca tcggggtgcg aatctcgtgg ctcatgttgg
     2701 ccagaaattc cgacttggcc cggctggcgg tagtggcagc agcttcggca cgcttgaggt
     2761 cggtgatgtc gtcaaacgac cagacccggc caatcacctc gtcaccaatc ttttgcgggc
     2821 gtgaaatctg cctgaaactt cgcccatcgg ccagttcgag gatttcgttg ctggccacct
     2881 gcggctggtc gtaaatggca ttgaccctgg caatgaactg ttcgggctgc cccatctggg
     2941 cggctgcaaa ggctcgcaga tgccgcttgt cgggcgcatt gagcaactcg ggcggcatgc
     3001 gccacagctc ggcaaaacgc tggttccaaa gcgtgacctg gcccccgctg tccaccgcca
     3061 gaacaccatg ggcggcagat tcaatggtgg cattgagcag cgataccgac cattgcaggg
     3121 cctgctggtc ggccttgctc tggctgatgt tgcgcaccag tgcaatcagc gtcacggcac
     3181 cgctgctggc atctttgagc agcgagcttt tggcgctgca ccacaggtag ctgccgtcct
     3241 tgcaaagtgc ctggtactca acgccatttt gttgcgagcc tgacgcaaac acctgcccga
     3301 tgttggccag gcagccagcc tgatgatccg ggtgcacaaa gcgctcgaac ggctgattga
     3361 gcacttcgtt caccgcatgg ccgaattcga ccgtccattg cggcgacaca tagtcaatga
     3421 tgcccgctgg cgtcagtgtc aacagcacat cgctgaggtt ttcaatcagc gacaggtagc
     3481 gctggcggct ggtacgtagc gcaacttcga tctgcttgcg caccgtgatg tcaccggcgg
     3541 taccctcaaa gccgacgacc ttgccggatt catctcgcag tgctgtgcca ttggtgttga
     3601 accagtgcca actgccgtcc tggtgctgcg tgcggtgctc cagaccttgt agcgatgccc
     3661 cggtggccat cacccgctgt atcgctgccc gactgtccgc cagatcatcc ggatggatga
     3721 tgtcggcaaa cggcttgccc aggatttttt cgaccgaatg ccccatcagc agcgtccagc
     3781 ttggcgagac aaaggtcaac aaacaactgg tattgatggt gtagatgatg tcgtggctgt
     3841 tatcaatcaa cctccgatac ctggcctcgc tggcctgaaa agcacccaca gcctgctgca
     3901 gactggcggt tttggccagt accatctgct caagcttgtc gcggtagttt tgcagctcat
     3961 actcgctggc cttgcgctcg gtaatgtccg agacgatgcc gaccagatac ggcagcccgg
     4021 ctcgccagac gatgctgctt ctgacgtagg cattgcgcag gctgccgtcc ttgtgccggt
     4081 gcaaggtctc aaaacttgat gttttggcag ccaccatgcc agccacaccg gtccgcgtgg
     4141 cttcggcatc gagttcgccc tggacatcgg ccagcgtcaa gcgggcaaac tcctcgcggc
     4201 tgtaacccag gtcccggcag gctgcgtcat tgaattcgac ataagccagc gtgctggtat
     4261 cgatcatcac catgccatca acggcctgcg tcacgatgct gcggtaaatc tcttcgcgcc
     4321 cggccagttc agcctgggcc ttttcgagct ttttggtcag cgcattggca ctgtcaagtg
     4381 ccaggcgggc ggcgaactcg ctgtcgcgca ggtccatctc ggcgagtttg cgctcggtga
     4441 tgtcctggat ggttccgcgc agcagcagcg gcttgccact ggcatcaaaa gtcagttcac
     4501 ctagcgctga gacccagcgc gtttttccgt ctgccgggcg gatgattttg tagtcttctt
     4561 tgaacttgcc tttggcagcc actacccggt ggtaatagcc gagcagttca gcctggcttt
     4621 ccggcgccac caggcggctc cagttgtcaa cggtcttggc aaaggacgcg tcaatgccaa
     4681 agatgttgtc cagcacaggc gagccttgcc agaggccggt ctgaacgtca gtgacatagg
     4741 tgccgatctg cccaatgcgc tgggcttcgt tcattgccca ggcattctgg agcagcattt
     4801 cggcgctggc agctcgctct gcttcgcgcc cctgcgccgc agccagtgca accattttct
     4861 gcgccaggtc gtctcggcgc tgccgcgccc ggccgatcaa tgctgcaacg aagcccagca
     4921 gggccgagag caacaggcca gccagcagca actgttgcgg cccttgtgcg acggcctggg
     4981 agccaaagcc tggtagcgcg cgaaagacca gcgtccaggt gcggccgcca aacaccagtg
     5041 gccgaacact ctcgaagccc gccgccaccg gcaactcaaa tgtgggtgtg gcatccgaat
     5101 caaacagcag cgtatcaagg ctctggctgt tgccgtcaaa gacttcgaga ccgatgttct
     5161 cgaagttctg cggcaatgcg tgctttaaca ggtcagccgc ccgaaatggc acattgaccc
     5221 agcccagcag gtgcgtctga cgcaaggcgg gtgtgtctgg cgctgtgccg cggcggtaaa
     5281 gcggctggta aatgacgaaa cctggcacac ctttgccctc atcctgcttc agggtgagct
     5341 tgcccgagag cgtcagtgcg ccgctgtcgc gggcccgctg catggcagca cgggctggca
     5401 aaacgctgaa agtgtcaaag ccgggcactc tgcggttgtc accgctaaac ggttcaatga
     5461 agaccaccgg cgcgtaagcc tcgcgcggct cggctgggtg gatctggtag tcggcctgac
     5521 catcacgacg taccccggcg aggtgctgat cgagcttgcc agccgggaca ttctcaacat
     5581 aggtcacagc agcaaagttg tgaaccccct ggtccagact gagggactca aaaaaagtgc
     5641 gaaagtcttg tcgcgtcacc tcggaagagg cattgaaaag gcctgtaaaa ctcttcagca
     5701 ccgaagcatt tgccgccagt tcggtttcaa tgctgcgctg ggcactggcc acctctcgca
     5761 ggaaatccgc gcgcagctca ttttctgagt tctggctggc ctgctgccac aacagccccg
     5821 ttgcggccat tgaaaccagc cacaccagca gtgccagacg gaccggcccg aagccggaca
     5881 ggtgccagcg catgggctag ctggcggcca attggcgacg ctgccaggcc cgcatcaggg
     5941 acttgctcat gatgctcgac gataccggtt tgatgcaata gccgtccacc cgttgatcca
     6001 ttgatttggt gaaatcagcc ggcagaatcg acccggtcac catgatcacc acgatctttg
     6061 gcaaaagctg acgcagttga tcgagcagtg cccagccgtc taaacctggc atgtagatgt
     6121 caagcaacac aaaatcgggg cggtactgtt gcgccagccg gtaggcaagg gctgcgtcct
     6181 gggcggtgtg gatacggttg acgcccaggc agatcaggac gttggtcatg atttcaagtg
     6241 aaaagtcgtc gtcgtcaacg atcaggtagc tgaaatcctt gatgtcttgt tccgtcatac
     6301 ttttctttct tggctgacta taaaaacaat agctgctgat gcaagtcggt caagcgctgg
     6361 atcctgattt tgcttaagtc gaatgccttg atctgcacaa tgatcagagc aaatttgtta
     6421 tttcgctaag tcgttgattt accgtcattc ccgcgcaggc gggaatctag tgcggtctgg
     6481 attcccgcct gcgcgggaat gacgaagatg ccaaaagaac aaatttaccc tgacacaatg
     6541 ataggttaat ttgcttccag ttgaagcttg tcgccatcaa aggtcggtgg cgtgacgcgg
     6601 ccatggggtc tgccgtggtt gggcacggca atgcgctggc aagcgttgct aacatgattt
     6661 tgtaataaga aaatgagaat taagtcgttc ctggtttgat cgcaaagccc caaaatccgc
     6721 tttattgcac tgacgcaatg gttttcgagg atatcaacta tgaacgcaag acaacgcttt
     6781 acccgccgca ttttcaacat cgccacatct gccttgctga ccggtgctgc tctgggcgcc
     6841 agcgcccagt cgccggtgac cctgctcaac gtctcctacg accccacccg tgaactctat
     6901 tcggagttca acgcggcctt tgccaagcac tggaaagcca agaccgggca agacgtgatc
     6961 atcaaacaaa gccatggcgg ctctggcaag caggcccgct cgatcatcga cggccttgac
     7021 gccgatgtcg ccaccttggc gctggccggt gacaccgatg ccctgcatga caacggtggc
     7081 tggattccca aagactggca aaagcgcctg ccgcataact cgtcgcccta cacctcgacg
     7141 attgtgctgg tggtgcgcca gggcaatccc aagggcatca aggactggga tgacctgatc
     7201 aaacccggca tcagcgtgat cacccccaac cccaagacct ctggcggtgc ccgctggaac
     7261 tacctggcag cctgggaatt cgccaaacgc aagttcggtg gccaggcaca agccaaagac
     7321 ttcatcgcca agctttacgc caatgttccg gtgctcgata ccggcgcgcg cggctcctcg
     7381 atcacctttg cccagcgtaa ccagggcgat gtcttcatca gttgggagaa cgaagcctat
     7441 ctgctggaaa aagagtttgg cagcaaggtc gatgtggttt acccctcgct gagcatcctg
     7501 gccgagccag ccgtgacggt ggtggacaaa aacgttgaca agaagggcac ccgtgccgtc
     7561 gccgaggaat atctgaaata tttgtacacc gaagagggac aagacttggc gggcaagcat
     7621 ttctaccgtc ctgccgtctc ggaaaaggcc aaggccaagt acggcaagca gtatcccaag
     7681 ctcaacctgt tcaccatcga ggatgccttt ggtggctgga gcaaggcggc caaggatcac
     7741 tttgcagatg gcgcttcgtt tgatcagatc tacacaaaga aataggcgaa tcatcacgct
     7801 cgccttcgct gtcacacacc cagattcagg gcaaacaaaa atgagcgttt tggtgaatgc
     7861 aggtagcctg gaggcgcatt cccgctgcgc tgccttgatg gaggccgtgg cacaacgcct
     7921 gcggtttcgt gttgtcttgg ttcacagact tgcacattcg tgaccggtcg ccgcaagcgt
     7981 tgggcgctgg ccatgatggt gctggtgatc ttccggtttt gcgttgacga gatcgccaag
     8041 ctgtttctga ttgccgccgc cccaccgccg cataggtggc gtggagccgg gctgatgcac
     8101 agagtcgcca gcgctttagc cgtcaggcgt tgctgcgggc actgatttgc accagtttgg
     8161 catgcgcccg gcgcagcgcg gaccggggcc gcagccagat tcggtgcgca atctgcaggc
     8221 agcagggata attttgcatt ttgttcagga gcgccacatg gccttgacca acacagcctt
     8281 tggcgacccc cgcgtcttgc tgcgccagat gtttgacgca gccgtggccg ccgcccaacc
     8341 ggcacggtgt ctgggcgcgc acctgcctgc cccacccaag ggccgcacca tcgtgattgg
     8401 cgctggcaag gcctcagccg cgatggcgcg ggcttttgaa gacctgtgga cagcgcggga
     8461 cagcagcccg cttgaaggcc tggtcgtcac ccgttacggc taccaggtgg cgtgccagca
     8521 catcgagatc gtgcaggcag cgcaccccgt gcctgacgcg gcaagccaac tggcggcgcg
     8581 gcgcatccgt gagcgggtca gcagcctgag cgctgacgac ctggtggtgg cgcttgtctc
     8641 aggcggcggg tcgtctttgc tggtggagcc aagtgagggc ctcactttgg cagataaaca
     8701 ggctgtgaac gccgcgctgc ttgcgtccgg tgccaccatt tcagagatga attgcgtgcg
     8761 tcggcacttg tctggcatca agggtgggca cttggctgcg gcctgtcacc cggccagact
     8821 gctcacgctg ctcatctcgg acgtgccggg cgactcgccg atggacatcg cgtcaggccc
     8881 cacggtgggt gattcgacca cctgtgcgga tgcgctggcc gttgcggatc gttaccaaat
     8941 caaaatgaca ccagccatgt gtgcgcgttt gatcagcggt gctggcgaaa ccgtcaagcc
     9001 cgacgatgtt cgcctgcagc acatcagcac acgcatggtc accgcgccgc agatggcact
     9061 cgaagctgcc gccggggtag ccagagcagc gggctacacc ccgcacattt tgggtgacag
     9121 cctggagggt gagtcgcgcg aggtcgccag ggtgctgggc ggcctggcgc ggcaggtggt
     9181 gcgccacggc cagccgtttc agcggccttg tgtgctgctc tcaggcggtg agaccacggt
     9241 cacgatcaaa ggcaaaggcc gtggtggtcg caacgtggaa ttcctgctgt cgctggcatt
     9301 gacccttgca ggcacgccgg gggtttatgg cctggcgggc gataccgacg gtgtcgatgg
     9361 cagcgaggaa atagccggcg cactgctcac accagacacc cttgagcgcg cctgggcgcg
     9421 aggcatgaac ccgcgcagct ttcttgacaa caatgatgcg cacacatttt ttcagcgact
     9481 tggcgattcg gtcattaccg gcccaacgct gaccaatgtg aatgactttc gcgcgatcat
     9541 gatagtttga gcccaagcga ctgaatgccg atggctcaaa caaaatgcgt ggcacggatt
     9601 gtgctaaacc gacagcctgt ccgtcggcgc gacgagccag gatcgggccc caagccgatt
     9661 gcccgacctg cattcatggc atcgatacgc gtcgcgttgg cgacaagggt cttggggcca
     9721 tccggccgat acagccgccc aactcgattt ctgaatcatt catgtcgctg ttaacttgac
     9781 ttgtctggcg tttgtaatgt ctgcgccagg gtgcctgaac acgaatgatc tggaaatggc
     9841 tttgcagcat tgacagcgag aatcgacccc agagaggcca gtttgtgcca gatttgccga
     9901 cccatctccg agttgccggg cagaaagccg gtgaaaatcg gccgctgcgg tcggttcgca
     9961 gcatgctctc tcagtctgcc aggcgcccag atggggtcaa atcagtgaga cgttggctag
    10021 acagaatggc gcaacgcccc agcattcacg tcagaccaca tccaaagcgg gttggcaagg
    10081 cttgttgggc tcaagtcacg gcattaggtt ggccatctgc tggagtggtt atgcaagctg
    10141 agtaagatgc tgtccacatt ggcttcgccg tgctgtagaa gtgagcgtat ccggggccgg
    10201 tcaaatggtt ttccgtattt ttagaactta cacatattat tatcgttgaa agagcctcat
    10261 aaatatttta aatacatcga tgttagatca ggtacgtttg actttggtta atacgtgctg
    10321 ttaattagtt atttgattat ttcatatcat tcgaagcata tttatgctca tgcgtaagtg
    10381 ctaatataat catattaata ccttagctct tctttgaggt tgttttgtta tgctcattgg
    10441 tataatatgt tttttctatt ttcttggttt gttttttgct ccctttccat gttggccaac
    10501 tgttttcaag ctctggcgaa ctctattgtt tgatagggca gatgcgactt cgaagatcag
    10561 gcagttcaca tttttgattt tggttatcgt ccgcgcacca ttttggactt ttttttggta
    10621 cattgacgaa ttactttttc cagaatatcg cgatcaactg atcgatcctg ttttcatcat
    10681 tggccagcca aggtgtggca caaccctgct gcatagaacc ttggcgcaag acaatcgaac
    10741 atttttcgca gttcgtcata tcgagtggcg ctatccattc atcagcgttc aaaaaacaat
    10801 taatttgttg ggcttgaata agtggctgga gaaagttgac tactggccaa aatcccaagc
    10861 aggacaaatg gcatcaaaga tgcacaaaaa caggctatct gactgggagg aggacggtat
    10921 tttttttgaa gaaaactttc tccatcattt cttcatctat ctgcgctttc cctatccaga
    10981 cctattgccg cacctcgatg cctatgcaac cctgcgtctt gaagatcaga agcgaatgct
    11041 tgaaatgcac cataaagtac tgaagaaagt gagctttctc cggggcgctc aaggccgctt
    11101 ctatctttcc aaggaagtta ccagccacaa caagctgcca gcacttgcag cgcgctaccc
    11161 aagcgcacga ttcattgttg taacacgaga gtcgagcaaa ttcatgtctt cgttaatgcc
    11221 attggtccgg atgtccactt ggtcgaagac tggagtcgac ccactcacgc tgccgaactg
    11281 ggaagacagt atgttgacca gaatgcgcag cgatagcctt aacctggttg agatttgcga
    11341 aaaagtcatt cctttagagc gtcaaaccag agtttcggca agtgacgtga tggtcaatgt
    11401 ggtagattct atttcgataa tttaccgcga gctaggtctg gttgcgagca gagaatttgt
    11461 caactatcta actgaactcg aggtcaatca agaggatcgg gagcgtggct atgaatattt
    11521 atcgctttct cccaaagggt tcgagcaata tgatgatttc gccaaaacca atgcgcaaca
    11581 atttcgaaaa aagatacacg aaataaaagg tcatcgcttg tgaaaattgg tcagacaaat
    11641 tggcaagctt aacattttga tgaattattc gaatcgattt aatcgggtca tcgtcatgat
    11701 caattgaaca tgccaggctt ccttggaggt agggcattca agatacaagt tgtaataaca
    11761 atcgccttga atttttgata ataatatttg caatgcgaaa tgcatgcaag ctgtggaaaa
    11821 aaatacctgc gttgaagcta ttgccagagg atcatgttgt ttttggcaga tcataatcgc
    11881 gacaggtgaa aattgccgca agcgagaggg acactgaaag tgggttcttc ttgatgacgg
    11941 atcaacccga tggcgcgtac ttcagcactg catccggccc aatccaatac cgcgaactca
    12001 agccttgaac gcgaaatcag gatgtggctt gagatgatct ttcggttccg gcgctttttt
    12061 gcctggccaa agttgcacgg aattggcccc cagtggctca aaagccttgc ccagcagagc
    12121 tgccacaccg tccaaagtcg gcgctgtgcg cggcacagct tcatgcagtg ggtctgggca
    12181 ggccaatccg tcaatgtcat ttccatatca acccgacata ttgctgcttt gccctgccgc
    12241 ggctgcagca ctgtctgcga tttcgcgcct gatgccgcat tgatctggat gccgaacaag
    12301 aggttgggga cgcaaaaatc aaacagcgtg atcaagctga cgactgcgat gcgttcttgc
    12361 gcttggccaa cgcgatctcc ctggtccagg tgattctccg ccgacccgag cgctggcgag
    12421 gtctgaaatg ctattgttgt tgtagctgct taggcaataa ggacaagcgc tggaggccaa
    12481 taacactgct aaacagaaat tgcattggca gcgtttgcca acctggttcg gtgaccctgt
    12541 acgctttccg acaaaaccgc acgtgatgta gcaaaaagcc tgccatccaa agcgcccgac
    12601 acgggtttat gtcgtgtttg ctgcttgccg ggtgtgtttt gggcctggta cggtcattgc
    12661 agataggcat ctgacagaac ctgatttttc aattgccaca tcaaccaccc ttaaggactc
    12721 ttcatgaccg ccaccctagc actcaatgcc attgacgaca ccgcgcttgc ccgccttgaa
    12781 gccgagggtc ggctgctcaa ccccttgcac aaggcgttaa ccggcaagcc cggtcgcgtc
    12841 ggatttcgtg gtgagctggc actgcgtttt gcgccaaagc tggccgacga agcccgccca
    12901 cccgaattgt tcagcgccca ggcgatggcg attgccaacg tcggcgagca gcacatcccc
    12961 ttctttgcgg gctacctgtt gagttttgcc tacctgaaag atgtcgccga ggtgttgggc
    13021 gatgcgctaa gtgccagtgg aaagtacttt ttgttttgca acaacattga tctgtcgaaa
    13081 aaataccagg ttgcctacca aggggtgacg ttctatgtgt tgccgattga tgaatcgacc
    13141 gtctataacg aactgctgga actgctctac ctggaaaaga acgacttgaa gaagaaagac
    13201 acagccggca aactcgacgc ggtcgccgat gcagcactca aattcaaagg gagctttgat
    13261 gccatcagct atgaagaagg attgaagctg atgggtccgg tgcgcaaccc caacgaaaac
    13321 cgcccggttt gagtgggcga ggatgacgca cttgaccagc ccagccgctg gtacggtaca
    13381 aacctggtgg ggttgccaac gcaattgctg gccagcactg cgttcaacag ccatcctctg
    13441 ccactgacga ttgccggcac ctgtgaaacg catcctggcc tgttcaccct gctggaaagc
    13501 agccagactc tgaccgaggc gcaagacatg tttgtccact atctgagcat tgcctttggg
    13561 ctgcgcaaac ccgaaccgca tgagctaagc agcatgggca agtccgagca gcggcgctgg
    13621 cgcagcagtt ggcgcaagtt gctgcaaggc tggggcatgg actcgaatgg tgccgcagga
    13681 gcagtgctca aaggctgggt agagagccgt tttggtttgc tgccgagctt tcacaaggca
    13741 ccgctggccc ggtttccgtc acccgcctgg atcacttacc tgcaggaaaa agcgtctagt
    13801 cggctcaaca acaacaatat ttaccagcag cttgaccttc tgtttgagtt ttgccaatgg
    13861 tcgctgcgtc gcattcgcac ccaatcccac tgcgcgccag cccagcacct gcgcctgtgg
    13921 cgcggcagca atcagtgcga agagcaggtc gttgccggcc ggctgcaaaa ccggcgctgc
    13981 acggtacgtc tgaacaacat tgtgtcattg agcctgtcgc cggaagatgc ctcgtgcttt
    14041 ggcgactggg tgtttgaagt gcagatgccg gtcagcaagg tgctggtgtt tcctgagctt
    14101 ttgccaggcc aggtgctgca gggcgagcag gaggtgctgg cgctgggtgg caattttgaa
    14161 gtgatggcgc gttatgcctg atttacttga cagggcattg ggggcttacc tggggtttgc
    14221 cattggcgat gcgctcgggg ctaccgtcga attcatgttg ccgcgcgaga tcgcttacca
    14281 atatggagtc caccgagaca tcattggtgg tggctggctc aaactggcct gcggtcaggt
    14341 caccgacgac accaccatga gcctggcgct gggcgaggcc ctattgatcg gcggcagcca
    14401 ggctttgagg catcatgccg atggcgacga cacgctggtt cgcgccattg gcgatgccta
    14461 tgtgcgctgg ttcaaggccc ggccggtgga ctgcggcaac acctgccgtc gcggcatcgt
    14521 gcgctacatg cgtgaaggtt cgctgcaagg cagcttgaac cctggtgacg gcggcaatgg
    14581 cgcgctgatg cgcaaccttc ctgcggcgct ggccaccctg ggtgacgagg cggcatttga
    14641 gcgtgtgtcg atggcgcagg cgcggctgac gcaccatcac ccgctgtcgg atgcggccac
    14701 actgggcctg ggccgactgc tgcatttgct gctggcagga tcagaccaca gcgcctgcca
    14761 gctttgggca gcgcagtgga ccactgacaa tccggctttt cgtttcaagc cgtaccgtgg
    14821 ctacgccagc gcctatgtgg ccgatacggt acaaaccgtg ttgcactacc tgacactttt
    14881 cgacgacttt gaggaggcac tggttgccac cgtgaaccag ggcgaagatg ccgacaccac
    14941 cggggcactg gtcggcatgc tggcaggcgc gcgctgcggt gcggcccgcc tgccgctgca
    15001 ctggctgcac cggctgcagc ccgacatggt tcgcacaatc accgagcaaa cccgcagcct
    15061 gctggcgcta gcaggctatt ccggttgatc gtgaccgtcc attgcggttt atctggccaa
    15121 tgaacatttc gccacgccag cgggctgatc aaactgaaaa tccgaaatct gcaggctgat
    15181 cgacgatggc tgctggcagg ccagcgacca ccgcttcaaa acgctggtgc gcgcatgcag
    15241 cacaccggta tgtcacattt gcggcagcac ggcactggaa aaatgtttgt cgcgcatcgc
    15301 cccggcgggc aagacccggg cgattcgcct ggcgcaccat tgcgctcgtt cggtccgcaa
    15361 agctacaaaa tgtagcggct caagtcttcg ttcttgctga gttcagccag gcgcgattcg
    15421 acataggcgg catcgaccat gatcgtctga cctgccaggt tggtagcatt gaagctcact
    15481 tcgtcgagca gccgctccat caccgttgac aagcgacggg caccgatgtt ttcggtgcgc
    15541 tcgttgacat caaaggcaat ctgcgccagc cgggtgatgc ccttgtcaga aaattcaacc
    15601 gttacctgct cggtggccag cagcgcctgg tattgcttga ccagcgacgc tgtggtctgc
    15661 gtcaggatgg ccacaaagtc ctgcaccgac agcgactgta gttcaacccg gatcggaaag
    15721 cgcccctgca actcgggaat caggtcgctg ggcttgctca gatgaaaggc acccgaggca
    15781 atgaacaaga tatggtcggt cttgactgtg ccgtacttgg ttgagacgct ggtgccctcg
    15841 accagcggca gcaggtcacg ctgcacgcct tggcgcgaca cttcagcccc gttaccctcc
    15901 gagcgtgagg tcaccttgtc aatctcgtcg atgaagacaa tgccgttttg ttccagcatt
    15961 tgcagcgcct gcgtcttgat gtcgtcttcg ttgaccagtt tggccgcttc ttcatcagcc
    16021 agcagtttga gggcctcgcc aatcttcagt ttgcgggtct gcttcttgtt ctggttgagc
    16081 tgaccaaaca tgccgcgcaa ttgttcggtc atctcctcca tgccggccgg gcccatgatc
    16141 tcaagctgcg gtgcggcatg cgccagatca acctcgactt cccggtcatt aagcaggcct
    16201 tcgcgcaatt tcttgcgaaa attctgacgc gccgtgctct caggttcgtt cgcttcaagc
    16261 acgccaaact cctgcctggg cattggaatc agaatgtcca gcacacggtc ttcggccacg
    16321 tcttcggccc gagctcgcac tttttgtgtc tcggttaggc gggtctgctt gacggctata
    16381 tcggccaagt cgcggatgat ggcatcgaca tccttgccaa cataaccgac ttcggtgaac
    16441 ttggtggcct ctaccttgat gaacggggca tcagccaggc gcgccaggcg gcgagcgatt
    16501 tcggtcttgc caactccggt gggaccaatc atcaggatgt ttttgggcgt gatttcgccg
    16561 cgcaggtcag catcaacctg ctggcgacgc cagcggttac gcagcgcaat ggccacggcg
    16621 cgcttggcct gctgctggcc gacaatgtgc ttgtcgagtt cggagacgat ttgttggggg
    16681 gtaagagatg acataaatca agcagtgggg aaaagtgcaa gcagccggat gtccgacaaa
    16741 gtgttgggga cagaacaaag gtgcttgcac gtcattctgg ctttgtcccg caatgggaca
    16801 gccgacgatc agttggggac agagcaaagt tcacttcccc gcagctccgc tgcgcacgtg
    16861 actcttggtc tgtcccgcaa tgtcctctaa ggtctctatc gtatggttca tgttggtgta
    16921 aatacagatt tcaccagcaa tttccaatga gcggcggacg atatcctggg cactgagttc
    16981 ggtgtgattg agcaacgcca tggcgctggc ctgagcgtag gccccaccag agccaatggt
    17041 gacaatgccg ttttcaggct ccagcacatc tccgttgccg gtgatgatca gggatgcctc
    17101 cagatcggcg accgccagca tggcttccag acggcgcaac actcggtcgg tgcgccagtc
    17161 cttggtgagc tcaatggccg cgcgcaccaa gtggccctga tgcttttcca gcttggcctc
    17221 gaagcgctca aacagcgtaa aggcatcggc tgtggccccg gcaaaaccag ccagcacctt
    17281 gccgtggtag agcttgcgaa ccttgcgcgc tgtgcctttg accacaatat tgcctaacgt
    17341 gacctgccca tctccaccaa tggcgacttg catgccgctg ggcgtgttgc gacgcacgct
    17401 gatgatggtg gttccgtgat attgatccat agaaataaag gcgtcctaat ttgttcgcac
    17461 aatgatgtgt gcgtgctcgt tgatgccgat caagttgaaa aacacggcaa tcagttgatg
    17521 gacatcacgc aattcaatgt gcccacccgt cgcttgcgca ttggccagcc agttcaagat
    17581 gctgccaccg gctgaaaaat caacccggat caaatctgcg caatagacga ccaaggtgtt
    17641 gttggcggca aaggccgttt gcagcgcctg caacacctgc gatgcatcac ctaacacctc
    17701 gccagtcaat gccagtgcct gattgggcga agcggtatcc tggtgcatgg ctggcaacag
    17761 ggcagtcgcc gcagggcgac tcagcgccgc ctgtgcagtc gctcctggtc cttcgatatg
    17821 ttgacagcga gcggcttgcc aagtgggggg cgacttttca taagtcacgc aaaagtctag
    17881 cgccagcaac tcgaactcat cgtgcagccg caggatgcgc atgagatcaa gtcgcagttg
    17941 ccaccagaac tgcgcgacat gggtgtcacc catgggggtg tggagccgca gcagatgatc
    18001 cagcgactca gtgccttcca gatgcagcac caagggcagc gtgcaccagg tcgccaacaa
    18061 cacagccagc ggctgggcgg cttgtggagt gatggttttg agggcgctcc agtccagcca
    18121 gcagggcgca aggctgcttt tgaccaagga ctgaagttgg gctacatcgg ctgcatcaag
    18181 ctctgcgggg caactccaac aggtgtaacc ggcacggtct gtctgcgaca actggaccgg
    18241 tgttgcgttt gcagtgtctg accctggaga ttgcaagcca gcaacccagg tcggcaccgg
    18301 acaggcaaac cggagcgaat actccgacgc gatttgatca aaactggccc gctgaccggt
    18361 tgcccgatac aggtctaaca gcgcactggc ccaggctctg accaactcag tgggtgctgc
    18421 gggtgtctgc aacgccatca gcagtaccga ctcagccccg ccgtcgtcac tgttggcaaa
    18481 ccgaatggcc gcctcttcaa gccttggatc gacctggttt tgctgtgcat ctgcgcaaat
    18541 aaaagccggg gtggtaagga cgccggtaac cgtctgtgct gtgacggctg ggactggcaa
    18601 ctggctgaca ggcggtgcct gttggctgtt ctcgccgttc tcgctttcga tcaggtcggc
    18661 tggcatggtg gtggcaaagg cgctgtcgat ccccatcggt tcttgcgccg ggttacctgg
    18721 ctgggcgact ggcgacgcaa gtccacggcc agccgctttc ccagacgcag gcccatcgcg
    18781 ccccttccac cactgtttgg acatttgcgc ttcaatttcg tcaattttct tgagcgtgtt
    18841 ggcgcgttct tggtaaaccg catagcccca actgtcctga tcggaagttg gcggtagtgt
    18901 ggcgctggtc gtgtccggca ctgcactgcg gcgcaacttg cgcaatttgt caaactcacg
    18961 cttgcgtacc gcatcgttgt actgcttgcg ctcaatcatc tgcttgagcg catgcttgct
    19021 gttgacttcc tcgctcactg cagggggctt atcgagatcc acccaatcta ccgtcgggtt
    19081 gagcacaaac ttggccactt tggagagaaa accggtacca atgccacgtg ttgccatgaa
    19141 aggagcccgc gcagtctgga cgcaatgcgt cagtctccaa acatcttctg cttgagttcc
    19201 cgacgctgtt gcgcttccag cgacagcgtc gcggttggcc gggccagcaa gcggccgacg
    19261 ccaatgggtt caccagtttc atcgcaataa ccatagtcgc ctgcgtcaat gcggttgatt
    19321 gattgctcga ttttcttcag aagctttcgc tcacggtcac gtgtgcgcag ctccagcgcg
    19381 tgctcttctt caatggtggc gcgatcagcc gggtctggca ccaccaccgt gtcttccctc
    19441 agatgctcgg tggtctcacc cgcattgttg agaatgccgt tcttcagctc gactaacttc
    19501 atgcgaaaaa acgccatctg ggtgtcgttc atgtaggcat cgtcaggcat ggcaatgact
    19561 tcggcatcct tgagatcggc cactggcttg gtcttccagt tgtccgccag agcaggatcc
    19621 tttttgactg ccgatgccag taccggggtg attggcgcga ccgcactgat ctgggtaaaa
    19681 ctggacttgg cagccgtgga tgccaccgac tgggccatcg acggcacagt cagttgcgcc
    19741 aggcgcgacg acggacggcc agatcgcacc ggcacgccca gcggggtcag ttgtggcgaa
    19801 ccggacactg gcttgaccac cgccgccggg gtgacccggg gcactttggc ggcatggggt
    19861 accggttcaa cttgggttgg gttagtcact tcttcgggtt tctttttaga catgggtgag
    19921 gctggcttgg cagatttggc ggtaaccaca ttgggggttt tggcagcagt agccaaattg
    19981 acggccgacc tggctgctgg ccggacttct gacttgaggg acttggcaac cccgccggat
    20041 tgagtggaga tggcttctgg gttgacgact tttactgtca cggtggacct cctcgcaggt
    20101 ttttctgtgc ttgccttggt gactgatgcc gacagtgagc tggcggcatc gacgggctgg
    20161 cttgccttgg gcgggcttac ctccccggcg cgcgagtgta cagctttgaa aacaggcaac
    20221 cccaacattt gcaaaagcga gccaaacata gagaatccgt ttcgctaaaa gatcaactta
    20281 aacaaggctt ttttccagac cctgcagaaa aatgtcgcta ggcaggtcaa tcccaatgaa
    20341 gaccatcttg ctgcagggct tttcgtccgg gccccacgcc gggcccagat cacttcccat
    20401 caactggtgc acgccctgaa aaatcacttt gcgcgcggtg ccctgcatat aaagaacacc
    20461 cttgtagcgc aacatgcgcg ggccatagat attaaccact gccccgagaa agtcttcgag
    20521 tctggcgggg tcaaaagccc ggtctgagcg gaatacaaag ctcttcacgt cgtcatcgtg
    20581 gaggtgatga tgaccgccct gatgggaagg atggtcgcag tgctcgccat ggtcatgctg
    20641 ctcatgggca tgctcatgga cctcttcttt aagaaaatcc gggtcaatgt cgagcttggt
    20701 attgaggtta aagccgcgca ggtcaaacac ctcagacaac gccacctcgc caaaatgaac
    20761 ggccttttgc ggtgcccgcg ggttcatgtg ggtcaggcgg tgttgcaggt cccgcgtctc
    20821 gtctggggac accagatcag tcttgctgat gaaaatctga tcggcaaaac cgacctggcg
    20881 acgcgcctcc tgacgatcat caagctgttt ttgcgcatgc ttggcatcca ccagcgtcaa
    20941 aatcgagtcc aacaggtagc tttcggcgat ctcatcgtcc atgaaaaaag tctgcgccac
    21001 cggacccggg tcagccagac cggtggtttc gatcacgacc cggtcaaagt ccagcaaccc
    21061 ctggcgtttt ttggcggcca gcaattgcag ggcttcgcgc aggtcttcgc ggatggtgca
    21121 gcaaatgcag ccattgctca tctggatgat ctgctctttt gagtcagtca gcaaaatgtc
    21181 gttgtcgatg ttttcttcac caaactcgtt ttcgatgatg gcgattttct gaccatgcgc
    21241 ctcggacagc acgcgtttga gcagcgtggt tttgccggaa ccgagaaaac cggtgaggat
    21301 ggtggcaggt atcaaactca tggtgcgtgc gtaatagaag gcagcgagtg taacgacgat
    21361 cagaccggct gactatttgc gcatcacgac aagccccttg aggtactcgc cttcggggaa
    21421 gttgatggtc atcgggtgat caggcgctgc gcccatgcgc tccaggataa acccgtcaac
    21481 gccggcctcg caacccgctg aggcgacaat tttgtgaaac agatcaacgc tgatgccgcc
    21541 tgagcaactg taggtaaaca acacgccacc gggctcgagc agcttgaagg ccagccggtt
    21601 gatgtccttg taggcacgcg ccgcacgctc ggcatgcgcc acggttgggg cgaacttggg
    21661 cgggtcaagc acgatggcat caaaactgcg gccctgttcg atgaactgac gcagggatgc
    21721 attcacgtcg gcatccatga aactgacgcg gtcggcagca aagccgttca gcgccacatt
    21781 ggcagcggct ttggtcagcg ccgggccgga tgagtcaatc gaggtgacat gggccgcgcc
    21841 accttcaagc gctgccaccg tgaagccgcc ggtgtaacag taacagttga gcacccgctc
    21901 aaacctcagg cgccgggcat actcggcaaa acgctggcgg ctgtcgcgct ggtccaggta
    21961 aaagccggtt ttgtgaccat cggcaatgtt gacggcaagc tgccagttgt gttcctgcag
    22021 ccgcagttcc acgtcacccg cgctgcgcag ccagccgcaa gcctcgggca gaccttcgag
    22081 cgtgcggctg ctggcatcgc tgcgttcata cagtttggtc aaaccggtgg tcgccagcaa
    22141 agcttcggca atcacgtctt tccagcgctc gaccccggct gaggtgaact gtgccaccag
    22201 cgtgtcgccg tagcggtcaa cgatcaaacc gggcaggcca tcggcttcgc cgtgaagcag
    22261 cctcagccca tcacttttga tgtcaaaacg tgctctagcc cttatggaac aagcgcaagc
    22321 agctataaaa aacgatgcat ccaggcgttg atgctgatca aaactccaga ccctggcgcg
    22381 aatcttggcg gccggactga acgctgccca ggccagaaac tgtccgtcgc tggactccac
    22441 ccgcacggtt tcgccggctt cggcgctgcc atcggcaatg gcagactcaa acaaccaggg
    22501 gtggcggcgc aacagcgaac gctctttgcc gggtttgaga cggatggttt tcatggatga
    22561 ctggacgctg cagcggcatc ccaaacaaac ggacgcaaat tgtcaatacg tgatggccat
    22621 cgccggcaca tcaacccgga tcatcggttt gcccaaagcg gcgctgactg cggcgacgta
    22681 ttctgccgcc catttggcat cgccgaaaag ggtcggcccg gcggcctggg tgacgaccag
    22741 aatcttgtcg gcggtcagca ccttgccgct ggcccgcagc ggttcgcatt caagcgcgac
    22801 gaactgctga tcaagccagg cgcgcgctgc atcatgcgcc agcggcacac cgtcgtgcac
    22861 atgaaaggtg aactcgtcat tgttgtccaa caccacgctg atttgttttt gcatacatca
    22921 cccgttgaat tgatagataa accgctattt tgaacccttt gacttgagat gcgcccgcgg
    22981 gtgcgctgca tcgtatgctt ttgccaaatg accaaagtcc agtcgggtat aaatttgtgt
    23041 ggtgctgatg ttggcatggc cgagcagctc ttgtacgccg cgcaaatcac tactcgactg
    23101 cagcaggtga ctggcaaacg aatggcgcag catgtgcggg tgcaccggca atgccaggcc
    23161 agcctgcaag ctgcggcttt tcaggcgctg ccagattgac tgcgccgtga gccgggtgcc
    23221 acgctggcca ataaacagtg ctgccggttc ggcagcggta ccggcagcga ccccatcggt
    23281 gacggatccc ggcgttgctg cagcaagcgc cggagcctgc ggggcatgac cctttggcat
    23341 ttgatcgcgc ag
//
LOCUS       DAOSLL010000003        22622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_2535_length_22622_cov_4.849736, whole genome shotgun sequence.
ACCESSION   DAOSLL010000003 DAOSLL010000000
VERSION     DAOSLL010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 22622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22622
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2535_length_22622_cov_4.849736"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2148)
                     /locus_tag="PLB25_00210"
     CDS             complement(<1..2148)
                     /locus_tag="PLB25_00210"
                     /inference="COORDINATES: protein motif:HMM:NF015397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HOE40051.1"
                     /translation="MIAKKIPNTKKRSTKSARAGGLAEYITQPELTNKIEKCISSEAV
                     NFISTYLKSQTAEMIALSQEAVKSKDPIDHWVLSWKSNERPTPEQAREAAAIFIKQCG
                     LEGHQYIAGLHDDTENMHLHIAVNRVHPDTCKVVKINKGFDKEAAQQSIAIIEKVQGW
                     SVETNARYRTNDRAELVIDPATKRPQIFTVGEKPIQPTTKAQAMEVQTGEKSAQRVGI
                     EQAAPILAKATSWKELHANMATTGMQYQRKGSGAVVQVGDELVKASDVARTASLSALQ
                     KRLGPYQPPQEIKPNEYHHHAKKPNPIALGKEAGNGMRKLSECNLAVLTNQGQTRRAG
                     VLHIDARPGGRPVDGLRRDARPGRTINTELTRQPLKDKQPGWNEYIAIRDTQKAAKTH
                     ETISLQMSHSAERATLAAKLKAERSVIFSGDWRGKGEAKNLLQSFTATQQAAEKLELQ
                     DRQRDERKALQDKYKPLPMYKQWKEQPQIVSLHVQPLIDQHITRDQQVTVAQTLRSLT
                     NSIDDRRHITYQLDRKNVFRDEGRTIAILDLKSDRGIAAALATAQQKFGQTLELTGSD
                     AFKKNAVAVAVANNLTCKFSDPALDKLREQLQHEKYEAAREASRAERERLAAEQLEKS
                     METGPLHSPIQQNDDVVAEWEAEKSSPERDSAHAEQLPPVDYLDEIHRQIELARAAAD
                     HRSMLAQSATVDGDHDAPASGTIVASNEEFVAVH"
     gene            complement(2145..2453)
                     /locus_tag="PLB25_00215"
     CDS             complement(2145..2453)
                     /locus_tag="PLB25_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobB mobilization protein"
                     /protein_id="HOE40052.1"
                     /translation="MVFQKIGDDELDAVVNVRLTSEEKERLRDDADMAALSMSALVRA
                     RYFGRPVVAHADQVMIKELRRLGGLLKKVHTDSDGAYSAETSAALYLVSEAIRKVSKS
                     "
     gene            2771..3169
                     /locus_tag="PLB25_00220"
     CDS             2771..3169
                     /locus_tag="PLB25_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40053.1"
                     /translation="MATKRELYEATEYIEKMDLMIAKLRTTKPGELTGKVGKNEILNM
                     RRDALQQLVNDGYTVNQISEAMKNDVFGILPKTITEIIGNKNKKPRVAKKVIDAVKPQ
                     KPVATKSADFANEKATTDNSKITVKKDEAL"
     gene            3170..3844
                     /locus_tag="PLB25_00225"
     CDS             3170..3844
                     /locus_tag="PLB25_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40054.1"
                     /translation="MSATIYLSGGGKGGVGKTMVSLALVDWLVNSDDKNKVTIVETDN
                     SNAEVYKAYENDTGIKKQLINMDAQVGWIALMNLMPEWAKSNEQVVVNTAARATEAIE
                     QNLNDLLLGAKQLGIAVKLLWTINRQRDSLNLINGVLKKCTVDTTIVKNLYFGSPDKF
                     VLFDKSELSKKVATINLPDLNDEVADKIYIDRLALSQVDQFKFGEKIALQRFKTDAAE
                     QFKKIK"
     gene            3859..4662
                     /locus_tag="PLB25_00230"
     CDS             3859..4662
                     /locus_tag="PLB25_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40055.1"
                     /translation="MALALRDSIEQLTGKKVTDQHIQKILAVASGMEIGQNDPMLLLF
                     LVLDHYNGLFNEAPKKINDAVNNSVKTAEREAELVVNDASRKVQTIVAGSLEPLATAA
                     FDKGVNKYIDDLTGQAESAARSKTMPVAIAAAAGVLLVGLMLGWGSGTWARLDADEKL
                     IAAAAVSQKTVAAQLAQKDAEAAATIANLKAEQATAIANLSAAKGWAGTPEGQLAYKF
                     FTAGGGVLAARCQAERWIIGKTGKGEKLCVPQVETFFGWKDGNIGWVIP"
     gene            5369..5998
                     /gene="parA"
                     /locus_tag="PLB25_00235"
     CDS             5369..5998
                     /gene="parA"
                     /locus_tag="PLB25_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family partition ATPase"
                     /protein_id="HOE40056.1"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARVLQLDGASVVLVDSDPQGSA
                     RDCSAARAEQPLSVIGIDRPTIERDLKSLAKTDFVVIDGAPQASDLAVSAIKAANFVL
                     IPVQPSPYDIWAAADLVDLVKQRIEVTDGRLQAAFVVSRAITGTKLGQEVADALAGYG
                     LPVLTSRIMQRVAYPSTAATGQTVFDIEPNGDAATDFLALKNELVQLLK"
     gene            6011..6220
                     /locus_tag="PLB25_00240"
     CDS             6011..6220
                     /locus_tag="PLB25_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40057.1"
                     /translation="MNANKKTTGLAAGRPSVSKQNLSMEDEPVLVRINAQVTEAEHQK
                     LKIHAAKSKTSISELLRAFIATLPD"
     gene            complement(6270..6470)
                     /locus_tag="PLB25_00245"
     CDS             complement(6270..6470)
                     /locus_tag="PLB25_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40058.1"
                     /translation="MIETIDALQWAGCATGVAGALLLALNIKRSGWGFVLLLISNGFW
                     TAFGSCTGLLTQPGAVLKFLLF"
     gene            complement(6467..7420)
                     /locus_tag="PLB25_00250"
     CDS             complement(6467..7420)
                     /locus_tag="PLB25_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOE40059.1"
                     /translation="MGRESTITFEQIAAVADAMKIDGVKPTSRAVRERLGNTGSMGTI
                     NKLLGRWKSGQERQISAALVLPPALQRVLLEFMDTELTAARTTLETELADQQQEAADL
                     ATENERQVIENDTQAELIEQLRSDVAAHQGRAGQLEADLTASRKEAVRERSGAELART
                     ELAKAQLHLEAMPRLEADLVALRNDLEKERKACRQAEQDAAVFAAKLEAAERRAKEAD
                     ARTEKASTVAAQIADKADAVARELVSANAAVQAGQARLESVAREIDDAKKAVATANAD
                     AKKSGESAAELRGQVEALQAQAIKPALEAAAKPAAKTAKKS"
     assembly_gap    8205..8304
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8368..9303)
                     /gene="trfA"
                     /locus_tag="PLB25_00255"
     CDS             complement(8368..9303)
                     /gene="trfA"
                     /locus_tag="PLB25_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid replication initiator TrfA"
                     /protein_id="HOE40060.1"
                     /translation="MATNENTKKPKTESEIQAMLANLKARGKPGPVVIPGDLMITAQP
                     PTVAQLPLWAEPVRGVPNAVLRSALFGAIKRGKRAYQQGVKKASVEGVTVIHTGPQLD
                     QADLDVWQQCLHLARTGGLGTRIQFTAGGFLKAIERGTGKSQHEWLKNAFRRLSSSVV
                     EVAEGKRAYFGPMLHRGARDEETGNYVIEMNPDIVKLFGADGWSSIEFEARRALKKKP
                     LSQWLHGFYSSHARPFPMKVETLHRLCGSEAKLLKHFRAELRDALDKLAEVTGWTHEI
                     DDNDLVHVAKTGTDSQQRHLIAKHGQKLIKKLTQK"
     gene            complement(9382..9660)
                     /locus_tag="PLB25_00260"
     CDS             complement(9382..9660)
                     /locus_tag="PLB25_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOE40061.1"
                     /translation="MKKVIRLQSYRQSLRELAAIVAKDNPRAAAKLRINIDDQVTQLA
                     DPNFPRKIGRVKGTFELVAHPNYIVILEESATTVTALLVVHARQQWPN"
     gene            complement(9657..10112)
                     /locus_tag="PLB25_00265"
     CDS             complement(9657..10112)
                     /locus_tag="PLB25_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40062.1"
                     /translation="MAITVDTIDRTTLEHLVEAGAVKGASIVGQPGGWGVVVHYGLTE
                     RVLAARRGAIRNFKKFETLVDYLKSIGLAKYQVDATEYEPAAPPSDRKRTDAAERMKD
                     AHAAVAYRNWLEGKVSAARAGMADGSNKPIPDAEWATERAEWEREAHGA"
     gene            complement(10327..12396)
                     /locus_tag="PLB25_00270"
     CDS             complement(10327..12396)
                     /locus_tag="PLB25_00270"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE40063.1"
                     /translation="MDTSNYRTAVDAIAIYTETDLAGTITHVNPKFCEISDYSKQELL
                     GQNHRLLNSGHHPSEFFEDMWRAITSGQVWRGDICNRAKDGQLYWVASAIMPLLDQAT
                     GLPRKYISIRFDITPRHLLQNQLAFQAEHDVLTGLANRQLLQQRLEHAMTQADLGLRQ
                     IGVCFVDLDGFKAINDRLGHACGDQLLIQVAQRLTDTVQTNDLVARLGGDEFVVVLTQ
                     LGSLTDARPIVERVLAALAMPFTLPSDTVNISASVGLTLYPHDKVSADALLRHADHAL
                     YSAKQAGRNRFEFFDVAHDTDTSAHYRILASVTHALKHHELVLHYQPKVNMRSGQVVG
                     FEALLRWQHPRDGLVPPLSFLPLVEETDLIIDIGEWVIDQALAQLASWGAAGHVWPVS
                     VNIAARHFHQSDFVQRLQALLARHPSVPHQWLDIEVLESVAMRDIAQVQRTIKACQAL
                     GVTFSLDDFGTGYSSLSYLKYLPTETLKIDRAFVRDILDDKDDLALTEAIITLSTVFN
                     RNVVAEGVELAEQGVLLMRLGCDVAQGFGIAKPMPASEVQAWALEYTPAPSWGAWADV
                     RWELSDFPLLVAQYDHLQWVKRVVMAVDGGNLGLTASQLTDHHQCRLGHWYYGHGMAR
                     YGHLAEFKALESVHQLVHQTGVEIIRLRDAGQMDEARVACAQLLGMKDGILKLLDGLG
                     KAVLRQH"
     gene            complement(12428..12874)
                     /locus_tag="PLB25_00275"
     CDS             complement(12428..12874)
                     /locus_tag="PLB25_00275"
                     /inference="COORDINATES: protein motif:HMM:NF025062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CZB domain-containing protein"
                     /protein_id="HOE40064.1"
                     /translation="MGVFKLPGGDSSAAVNRTPALLSRSSVNESNIDLDSAIKTHADW
                     KLKLRNASAAHDTLDAATLSRDDCCVLGKWLHGSGRASYGHLPQFVQLIDRHADFHQE
                     AGRVATSINQGRYEQAQSMLGAGTGFSNASNAVGVAIKKLREATGL"
     gene            complement(12857..13747)
                     /locus_tag="PLB25_00280"
                     /pseudo
     CDS             complement(12857..13747)
                     /locus_tag="PLB25_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923929.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(13988..15073)
                     /locus_tag="PLB25_00285"
     CDS             complement(13988..15073)
                     /locus_tag="PLB25_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOE40065.1"
                     /translation="MYKVAIQPHWEITSDADVPLDTAVLLELLVAIKKTGSIAQAAQT
                     IGLSYRYAWGLLRDVEKLFGDTLMDKGRGRGTVLTPLAEKLIWADRRVAARLSPMLES
                     LSSELELELNKMTAGKSRTVRLHASHGFAVAALLNDINNAKLPIELRYRNSTEAVAAL
                     SRQECDLAGFHVPLGDFEKPAVALYAQWLNKKTHRLIHLAVRSQGLFVATGNPQGIHG
                     LQDLTREGVRFVNRQVGSGTRMLLELMLEGASLSPNDINGFENTEFTHSAVAAFIASG
                     MAEVGFGVHTAAQRFGLEFIPLVQERYFFALPVSALDDPLIQQVIGLLQSASFREEVD
                     TLAGYDATDTGKILSLAEAFGSSDPTN"
     gene            15221..15715
                     /locus_tag="PLB25_00290"
     CDS             15221..15715
                     /locus_tag="PLB25_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HOE40066.1"
                     /translation="MAKKPGNTLPAFDRRAVEAIIGQCKAMPGAMLPILHGVQDRVGY
                     IPPETVQLIADALNVSRAEVHGVITYYHFFRQTPPGRHVVQICRAEACQAVGGEALAV
                     HAKAVLGCDFHETTTDGKFTLEAVYCLGQCACGPAVMFGNDLHARVSSGKFDRLLDEK
                     RSTP"
     gene            15712..17265
                     /locus_tag="PLB25_00295"
     CDS             15712..17265
                     /locus_tag="PLB25_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOE40067.1"
                     /translation="MKTTVYVPCDSTAIALGANNVADAIAREAQARGIDIRIVRNGSR
                     GMFWLEPLIEVATPQGRMAYGPVEVEAVASLFDSGFALGAIHPLALGLTDEIAFLKNQ
                     ERLTFARVGIIDPLSLDDYLAHDGYAGLQRAIGMAQADIVQEVLDSGLRGRGGAAFPT
                     GIKWKTVLGAQAAQKYIVCNADEGDSGTFSDRMVMEDDPFMLLEGMTIAGLAVGATQG
                     YVYVRSEYPHAIAALTEAILIARENGYLGPNVLDSGQAFELEVRKAAGAYVCGEETAL
                     LESLEGKRGIIRAKPPLPALEGLFGKPTVINNVISLAAVPLILARGAAFYQNYGMGRS
                     RGTLPFQIAGNIKHGGLVEKAFGLTLRELLIDFGGGTASGRPIRAVQVGGPLGAYLPE
                     SLFDTPMDYESFIAIGAGVGHGGLVVFDDSVDMAKQARYAMEFCAIESCGKCTPCRIG
                     STRGVEAVDKIIANENRPQQIHLLRDLCDLMLNGSLCAMGGMTPFPVLSALNHFSEDF
                     GSTPLQKAA"
     gene            17298..20171
                     /gene="fdhF"
                     /locus_tag="PLB25_00300"
     CDS             17298..20171
                     /gene="fdhF"
                     /locus_tag="PLB25_00300"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016832027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HOE40068.1"
                     /translation="MNQLNELDYGTPARVSEKTITIEIDGVQVNVPAGTSVMRAAVDA
                     GVNVPKLCATDSLEPFGSCRLCLVEIEGRRGYPASCTTPCEPGMKVRTQTPKLAEIRR
                     GMMELYISDHPLDCLTCPTNGNCELQDMAGVVGLRDVRYGYDGKNHLKLAKDESNPYF
                     TYDASKCIVCNRCVRACEETQGTFALTISGRGFDSRVSAGQDQSFMESECVSCGACVD
                     ACPTATLTEKSVIKMGQAEHSKITTCAYCGVGCSFKAEMKGNEVVRMIPNKNGHANHG
                     HSCVKGRFAWGYATHRDRMLKPMIRKAITDPWQEVSWDEAVNYAASEFRRIQAKFGKD
                     SIGGITSSRCTNEETYLVQKLVRAAFGNNNVDTCARVCHSPTGYGLKQTLGEAAGTQT
                     FNSVMKADVIMIIGANPAAAHPVFASQMKRRLRQGAKLIVIDPRTTEMVKSPHIAADF
                     HLKLMPGTNVAVITALAHVIMSEGLFDAAFIAERCEEQSFQDWQKFVLQPDNSPEAVA
                     AITGVSSEEIRGAARLFAAGKNGAIYYGLGVTEHSQGSTMVMGIANLAMATGNIGREG
                     VGVNPLRGQNNVQGACDMGSFPHELPGYRHISDTTVRTEFEEAWQCTLDPEPGLRIPN
                     MFDAAVDGTFMGLYCEGEDIVQSDPNTQHVTAALAAMECIVVQDLFLNETAKYAHVFL
                     PGSSFLEKDGTFTNAERRISRVRKVMPPKAGKSDWEVTVALANALGYPMNYQNPSEIM
                     AEIAALTPTFRGVSYQKLDELGSIQWPCNDAAPEGTPTMHIGEFVRGKGKFINTQFFA
                     SAEKVSRKFPLILTTGRILSQYNVGAQTRRTDNVQWHSEDRLEVHPYDAEERGIKQDD
                     WVGIESRAGQTVLRATITNRVQPGVVYTTFHFPESGANVITTNNSDWATNCPEYKVTA
                     VQVMPVTQPSEWQRKYNRFSSEQVGLATGAVTAGEQSLAVVGK"
     gene            20192..21037
                     /gene="fdhD"
                     /locus_tag="PLB25_00305"
     CDS             20192..21037
                     /gene="fdhD"
                     /locus_tag="PLB25_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HOE40069.1"
                     /translation="MNAITSPERATLVQVSVEKWRNDQRRLCLNTLAEEVPIALEYNG
                     ISHAVMLASPFNLEDFALGFSLSEGILADSSELFDCEVSATKNGIQVQMQIASGRFAE
                     LKSKRRNLTGRTGCGLCGAETLEQAIRRPPVVNSTALFSAEALYAGMDAMQMHQHLQH
                     QTGATHAAAWLGSDGRLELVREDVGRHNALDKLIGAMASEKRSFSAGAALITSRASYE
                     MVQKAATLGIGFLAAISAPTSLAVQLAKEAHVTLLGFVRHQSHVVYTQPLRLECGKLE
                     SRSVS"
     gene            21034..21258
                     /locus_tag="PLB25_00310"
     CDS             21034..21258
                     /locus_tag="PLB25_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014005991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HOE40070.1"
                     /translation="MNADNLVIMANQIGSFFETMPDRQQALTDIAGHIKRFWEPRMRR
                     SLLKHVDEYAGVQLKDIVLEALRVHRASIL"
     gene            21646..>22622
                     /locus_tag="PLB25_00315"
     CDS             21646..>22622
                     /locus_tag="PLB25_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOE40071.1"
                     /translation="MNFTFKQLEYFVAVADCGTITGAAEKIHVSAPSISVAIAQLEES
                     LEIRLFTRQSSGLQLTTAGDEVRRRVRTILEETRGLQDISNTVTGELRGKISLGCLTT
                     LAPLLLPEVCQTFSRIHPAVSVEPIDGSQDQLIGYLRRAVIDLVITYDMHIPQDLWFE
                     SLVGRHPLIMLSPDHRLAQAPSVEMKDLESDPYILLDLPISRDYFLSIFEKTGITPDI
                     KMRTTNFDVVRTMVANGYGVSISVVHPKNEAALDGKPIIYKPISNPLPLLNIGTLSRR
                     TDFTSIATSLKRHIQSLITSSAIPGMRLIDHHQVPTFGISTQGFRGGKG"
BASE COUNT         4884 a         6086 c         6346 g         5206 t
ORIGIN      
        1 gtggacggcc acaaattcct cgttcgacgc gacgatggtg ccgctggccg gggcgtcgtg
       61 gtcgccgtcc acagtggcgg attgcgccaa catgctccgg tggtcggctg ctgcgcgggc
      121 cagctcgatc tgccggtgga tttcgtccag gtagtcaact ggcgggagtt gttcagcgtg
      181 tgcgctatcc cgttccgggg atgatttctc agcctcccac tcggccacaa catcatcatt
      241 ttgttggatt ggggaatgca gggggccggt ttccatggat ttctcaagct gctcggctgc
      301 aaggcgctcg cgctcggcgc ggctggcttc tcgtgctgct tcgtacttct cgtgctgcaa
      361 ctgctctctg agcttgtcca gggcggggtc tgaaaacttg catgtgaggt tattggccac
      421 ggccacggcc acggcgtttt tcttgaaagc gtcagagccg gtgagttcaa gggtttgccc
      481 gaatttctgc tgtgcggtcg ccagtgcggc ggcaatgccc cggtctgatt tcaaatccaa
      541 aattgcaatc gttcggcctt catcccgaaa aacattttta cgatcaagct ggtacgtgat
      601 gtgtcggcgg tcgtcaatgc tgttggttag tgacctgagc gtttgagcaa ccgttacttg
      661 ctggtcgcgg gtgatgtgtt ggtcaatgag cggctgaacg tgcaggctga caatctgcgg
      721 ctgttctttc cattgcttgt acatgggcaa cggcttgtat ttgtcctgca acgcctttcg
      781 ttcatcgcgt tgacgatctt gtaactccag tttttcagcc gcttgctgcg ttgctgtgaa
      841 gctctgtaac aagtttttcg cctcgccttt gcctctccag tcgcccgaaa aaataactga
      901 tcgctcggct tttagcttcg cggccagcgt agcacgttca gcgctgtggc tcatttgaag
      961 tgaaatcgtc tcgtgcgttt tggctgcttt ttgggtgtcc cggatggcaa tgtattcatt
     1021 ccatcctggc tgtttgtctt tcagtggctg acgtgttaat tcggtgttaa tggttcgtcc
     1081 gggtcgggca tcccgtcgca gtccgtcaac aggtcgtcca ccaggacgtg catcaatgtg
     1141 cagtacaccc gcacgccggg tttggccttg atttgtaagg actgccagat tgcattcgga
     1201 cagcttgcgc ataccgtttc cggcctcttt tccaagcgca atagggttcg gctttttggc
     1261 gtgatggtgg tattcattgg gtttaatttc ctgtggtggt tgataggggc caaggcgttt
     1321 ttgcagggct gagaggctgg cggtgcgcgc tacgtcgctg gctttcacca actcgtcacc
     1381 aacttgcacc acggcaccgc tgcctttgcg ctggtactgc ataccggttg ttgccatgtt
     1441 ggcgtgtaac tctttccaac tggtggcctt ggcaaggatc ggggcggctt gctcaatgcc
     1501 aacgcgctgc gctgacttct cgccggtctg cacttccatc gcctgggctt tggtggttgg
     1561 ctgtatcggc ttttcgccta ccgtgaatat ctgagggcgc ttggtggccg ggtcaatgac
     1621 aagctcggcg cggtcgttgg tgcggtagcg ggcgtttgtc tcaacgctcc agccctgcac
     1681 cttttcaatg atggcaatgg attgctgcgc ggcttctttg tcgaagcctt tgttgatctt
     1741 gaccaccttg caggtgtccg ggtgtacccg gttcacagca atgtgcaagt gcatgttctc
     1801 ggtgtcgtcg tgtaggccgg cgatgtattg gtggccttca aggccgcact gcttgatgaa
     1861 aatggcggcg gcttcgcggg cttgttcggg tgtcgggcgt tcgtttgatt tccaactcaa
     1921 aacccaatgg tcaattgggt ctttggattt cacggcttcc tgtgaaagcg cgatcatctc
     1981 ggcggtttgc gatttaaggt aggtgcttat aaagttgacg gcttcgctgg aaatgcactt
     2041 ttcaatcttg ttcgttagtt cgggctgggt gatgtattcc gcaagcccgc cagcacgggc
     2101 agatttcgtg cttctctttt tggtgttggg tatcttcttg gcaatcatga cttgctgact
     2161 tttcggatgg cttcgctcac caggtacaac gccgccgatg tttcggcgct gtaggctccg
     2221 tcactgtcgg tatgcacttt cttgagtaaa ccgcccaagc ggcgcaactc cttaatcatc
     2281 acttggtcgg cgtgggcaac cacgggacgg ccaaagtacc gggcacgaac aagggcactc
     2341 attgacaaag cggccatgtc cgcgtcgtca cgcaggcgtt ccttttcttc ggatgtgagg
     2401 cgaacattga caacggcgtc aagctcgtca tcacctatct tttgaaaaac catgggtggc
     2461 tccagttgcg gggttgttgg atgttggaaa gcgggtcaag ggcagagcca ttgcaggggt
     2521 gagtgtcaat gtgagaaggc gaagccgccg aacgttgata cgtaccttgg cgcacatgga
     2581 aaaacagtag gtttttatat gtgtgacaag ggccatcctg tacatttctt ttgtgttaac
     2641 gatatctaat attctaagaa cgatacatgt aaatgcaatc tacaaattca ctttaaagca
     2701 acgttcaata gaacgatttg attatttgag tcctagaatg ctataacgat taacttcatg
     2761 aaagacacat atggcaacta aacgcgaact ctacgaagca accgaatata ttgaaaaaat
     2821 ggatttgatg attgccaagt tacgaaccac aaagccgggg gaattgacag gcaaggtcgg
     2881 caaaaacgaa attctcaata tgcgccgtga tgcacttcaa cagttggtga atgatggtta
     2941 cacggtaaac caaatttcag aggcaatgaa gaatgatgtg tttggtattt tgccgaaaac
     3001 gattacagaa attattggaa acaaaaacaa gaagccacgg gtagctaaaa aggtgattga
     3061 tgcggttaaa ccacaaaagc cagttgcaac aaagtcggct gattttgcta atgaaaaagc
     3121 aacaactgac aattcaaaaa ttacagttaa gaaagatgag gcactttaaa tgagcgcaac
     3181 gatttacctc agtggcggtg gcaagggcgg cgttggtaaa acaatggtgt ctctggcttt
     3241 ggtcgattgg ctggttaaca gtgatgacaa aaataaggta acaattgttg aaactgacaa
     3301 cagcaatgca gaggtttaca aagcctatga aaacgacact ggaataaaaa aacagttgat
     3361 caacatggat gcacaagtcg gctggattgc actcatgaac ttgatgcctg aatgggcgaa
     3421 aagtaatgaa caggttgttg taaacacggc ggcaagagca acagaagcca tagaacaaaa
     3481 ccttaatgat ttgcttcttg gtgctaaaca attgggcatt gctgtaaagc ttctttggac
     3541 gattaaccgc caacgcgaca gtctgaattt gatcaacggt gtacttaaaa aatgcacggt
     3601 tgatacaact attgttaaaa atctttactt tgggtctcct gataaatttg ttctttttga
     3661 caagagcgaa ctttcaaaga aggtagcaac aatcaattta ccggatttga acgatgaagt
     3721 tgcagacaaa atttatattg atcgtttggc attgagtcaa gttgatcaat tcaagtttgg
     3781 cgaaaaaatt gcgctgcaac ggttcaagac agacgcagcc gaacagttca aaaaaatcaa
     3841 gtaaatcagg ggaaccaaat ggcattggca ttgagggatt caatcgaaca actgactggc
     3901 aagaaggtta ctgaccagca cattcaaaaa atactggcgg tggcttctgg catggaaatt
     3961 ggtcagaatg acccaatgct gctgctgttt ttggttcttg atcactacaa cggtttgttc
     4021 aatgaagcac ctaaaaaaat aaatgatgca gttaacaatt cagtaaaaac ggctgagcgt
     4081 gaagctgagt tggtagtgaa cgatgcatct agaaaagtgc aaactattgt tgctggttct
     4141 ctcgaaccat tagctacggc ggcatttgac aaaggcgtta ataaatacat tgacgacttg
     4201 acgggtcagg cagaaagtgc ggcacggtct aaaacaatgc cggttgcgat tgctgcggcg
     4261 gctggcgtgc tcttggtcgg tttgatgttg ggttggggca gtggcacctg ggcgcggttg
     4321 gacgcagatg agaagctgat tgctgcggcg gctgtgtcac aaaaaactgt tgcggctcaa
     4381 cttgcacaaa aagatgctga ggcggcggca acgattgcaa atttgaaggc tgaacaagcg
     4441 acggcgattg caaatttaag cgcggcaaaa ggttgggcgg gcactccaga aggccagttg
     4501 gcttataaat ttttcacggc gggcggcggc gtgctggcgg ctcgctgcca ggctgaacgt
     4561 tggatcattg ggaaaacggg taaaggggaa aaactctgtg tcccacaggt tgaaactttc
     4621 ttcggctgga aagatggaaa tattggatgg gtgataccgt gattttttct aaaaacgatt
     4681 caaacggcct acgggccgtt ttttcatgtt gctctcaggg ccgttttttc atactcaagc
     4741 ctaacctatt gaccgggtgc ttaaagtggc tggaatggcc gaatgcaccg cttcgcgggt
     4801 tggttggatg gaatgcaggt gaagttatgg cgaagccatg tagccgaagg cggtacggct
     4861 gttcagtggt ggcttgctcc tggtggtgtt ggattggcgt tgccttcagt gccgctgcaa
     4921 gacctttctt cctgatcgac ggatattggg atgcaccttg tgtgccgggc gaagccctga
     4981 cggacacgaa gttccgtctt gcatttgggg tgtctgtgcg gccccttggg gcctattgat
     5041 ggtgttgtag gttgatgcgc cgccaggtgc attcgggggc accgtgccta gcacgggggg
     5101 cgctctgcgc cccggcgtca gccgtggtgt tgtgttggta tgtaggggga accgcgacag
     5161 cggggcggca cgcctgaatt gggtgggcgg ccgcagaggc ggggagggcg aagcccttcc
     5221 gagtgcgtag cacgcgctgg ggtttgcagg gtgcatgtga aagtacgtgt tttttataca
     5281 gtcttttttt gtggtgtgac gaagtattta ttcttttact caaaaacaca tatacttctt
     5341 tatgttttag tgtaaatgaa ggaacctcat gaaagtgatt gcagtcttaa atcaaaaagg
     5401 cggcagtggg aaaaccacca ttgctacgca cttggcgagg gtgttgcagc tcgacggtgc
     5461 aagcgtcgtg ctggtggact cagacccaca aggcagcgca cgcgactgtt cggcagctcg
     5521 tgccgaacag cctttgtcgg tgattggaat cgaccgcccg acaatcgaac gagatttaaa
     5581 aagcttggca aaaactgatt ttgttgtgat tgacggagcg ccccaagcgt cagatttggc
     5641 ggtgtctgca atcaaagctg ctaattttgt tttgatccca gtccagccga gtccctacga
     5701 tatctgggcg gctgcggatt tggttgacct ggttaaacag cggattgaag tcacagatgg
     5761 gcggctgcag gctgcattcg ttgtctcgcg tgctattacc ggcaccaagc ttggccaaga
     5821 ggttgccgac gcactggctg gctacgggtt gcccgtatta acaagccgga tcatgcagcg
     5881 agtggcttac ccaagcacgg ctgcaaccgg acagaccgtt tttgacattg agccgaacgg
     5941 cgacgcagcg acagatttcc tggcactcaa aaacgaatta gtgcaattac tcaaataagc
     6001 aaaatagcaa atgaacgcga ataaaaaaac taccggattg gctgcgggcc gtcctagtgt
     6061 cagcaagcaa aatctatcaa tggaagatga gccggtgctt gtacgcatca atgcccaagt
     6121 gacagaagct gaacatcaaa agctgaagat tcatgctgcg aaaagcaaaa cgtctatctc
     6181 tgagctactt cgggcattca tcgcaacact tcccgattga tgattttatt catttactta
     6241 aaagcatttt tatgcattaa tttatttgat taaaagagta aaaatttaag cactgcacca
     6301 ggttgcgtca acaacccagt acagctacca aatgcagtcc aaaagccgtt ggatatgagg
     6361 agcagtacga aaccccaacc cgagcgcttg atgttgagtg ccaggagcaa cgcaccggcc
     6421 acgccggttg cacagccagc ccattgcagg gcgtcaattg tttcgatcat gattttttgg
     6481 cggtcttggc tgctggtttg gcggcggctt cgagtgctgg cttgattgcc tgggcctgca
     6541 atgcttcaac ctggccgcgc aactcggcgg cggattcacc tgattttttt gcatcggcgt
     6601 tggccgtggc cacggccttt ttggcatcat ctatttcgcg ggcaacgctc tccaagcggg
     6661 cttgtccagc ttgcacggca gcgtttgcgc tcaccagctc acgggccacc gcatccgctt
     6721 tgtcagctat ctgggcggca acagtcgagg ctttttctgt acgggcgtcg gcttcttttg
     6781 cgcggcgctc ggcggcttcc agctttgcgg caaacacggc agcgtcttgc tcggcttgtc
     6841 ggcaggcttt gcgttctttt tctaaatcgt ttctcaaagc taccaaatcg gcttccaggc
     6901 gcggcatggc ttcaaggtga agctgggcct tggcgagttc cgtgcgggcc agctcggccc
     6961 cgctgcgttc gcgcacggcc tcttttcggc ttgccgtcag gtctgcttcc agttgaccgg
     7021 cacggccttg gtgggcggct acgtcagagc gtagttgttc gatcagttcg gcttgtgtgt
     7081 cgttttcgat tacctggcgt tcgttctcgg ttgccagatc ggcggcttcc tgctgctggt
     7141 cggccagttc ggtttcaagc gtggtgcgtg ctgcggtcaa ttcggtatcc atgaactcca
     7201 acaacacccg ctgcaatgcg ggcggcagta ccagggcggc gctgatttgt cgctcttggc
     7261 cggatttcca gcgcccaagc aacttgttga tggtgcccat gcttcccgtg ttgcccaagc
     7321 gttcgcgcac ggctcgggat gttggcttga caccatcaat tttcattgca tcagcgacag
     7381 ctgcgatttg ctcaaacgtg attgttgatt cacgacccat gacacactcc ttaattgtta
     7441 cgttacgtta cgttacgtta cgttattata tgattgttat gatacgttat gctgcgaatt
     7501 gagaaaaata tttttaggcg cggaaaatgg cggcgaccag caaaaccttt aaaaacggcc
     7561 ttgggcgcgt tcaaaaggcg cgtggcaccc aatcccttta gatttttcgg aagatcgcag
     7621 gaacggctgt tttatgccgc ttgcggcatg tagcgaagcg gtacgggtgt tcagatgatg
     7681 gcttgcacct ggatgtgttg gattggcgct gttcgcactt cgcttcaccc cttcgccatc
     7741 ctgataaacg gatggtggga acccccttgt ggggtgggcg aagccctgac tgcgcctgtg
     7801 ttgtgctgtg gccgtggtga tctgttggcc tgtgaaccga cacgaagtgc ggttcggggg
     7861 tgcggattcg atccctaggg gtcacttaat ggtatttggg tgagtcgtag gcgagggcga
     7921 agccctggag cacacgaagt gagcgacttg ggggctctgt ctctaccccc cttgcggggg
     7981 gtctggagtt ggtgccttga atgagaagca cggtgcgcgg gtcgcacgcg gtattaaaag
     8041 aagattaaga gaggattagg ggcagttaat aaaacttcag gaacctagca tccatgcggg
     8101 tttgctgggt tttctggcct gtggatgggt cggtgctatg ccgtgccacg gtcggtgcta
     8161 tgccgtgcca cggtcggtgc tatgccgtgc cacggtcggt gctannnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnntgccgt atggctaagt gagaacgaaa aaacgccccg
     8341 aagggcgttt cagtcggcgc tatgccgtta tttttgggtt aatttcttga tcagtttttg
     8401 accgtgtttt gcgattaggt gccgttgctg gctgtccgtg ccggtcttgg cgacgtgcac
     8461 cagatcgtta tcgtcaatct catgcgtcca gcctgtgacc tctgccagct tgtccagcgc
     8521 atcccttagc tcggccctaa agtgtttcaa aagcttggct tcgctcccgc acaatctgtg
     8581 cagggtctcg actttcatgg ggaatggcct tgcatggctc gaatagaagc cgtgcagcca
     8641 ttggctaagg ggtttttttt tgagcgccct gcgggcctca aattcgatgc tagaccaccc
     8701 atccgcacca aacagcttca caatgtctgg attcatctcg atgacatagt tccccgtctc
     8761 ttcatcacgt gcgcctctgt gcagcatcgg cccaaagtag gctcgcttgc cttctgccac
     8821 ctcgacaaca gacgacgaca ggcggcgaaa ggcatttttg agccattcat gttggctctt
     8881 accagtgccc cgctcgatgg ctttcaaaaa acctcctgcg gtgaactgga tgcgcgtgcc
     8941 aagcccgcca gtgcgggcta agtgcaagca ttgctgccaa acgtctaaat ctgcttgatc
     9001 aagctgtggg ccggtgtgaa tgacggtcac gccctcgacg ctggcttttt tcacgccctg
     9061 ctggtatgcc cgtttgccac gcttgattgc tccaaacaaa gcagagcgaa gcactgcatt
     9121 tggcacgccg cgcactggtt cggcccaaag tggcaactgt gcaacggtcg gcggctgtgc
     9181 cgtgatcatg aggtcgccgg gtatgacaac tgggccgggt ttgcctctgg ctttcaaatt
     9241 tgcaagcatg gcctgaattt cagactctgt ttttggtttt ttggtattct cgttagtagc
     9301 catttttgac tcttatacag ttggatttgg ttaggctttg ccgggtggtt tcgacacctg
     9361 gcaaagccgc ttttcaattt gtcagtttgg ccattgctgt ctcgcgtgca cgacgagcaa
     9421 cgctgtcaca gtcgttgcac tctcctccag aatcacaatg taattcgggt gcgcaaccaa
     9481 ttcgaacgtg cctttgacac gcccaatttt tcgcgggaaa ttcgggtctg caagctgcgt
     9541 cacctggtca tcgatgttga ttcgcagttt ggcggcggct cttggattgt ctttggccac
     9601 tattgctgcg agttcccgca agctttggcg atacgactgc aatcgaatga cttttttcac
     9661 gctccgtggg cctcacgttc ccattcggcg cgttcagtgg cccattctgc atccgggatg
     9721 ggcttgttac tgccgtcagc catcccagcc cttgcagcgc ttaccttacc ttcgagccag
     9781 ttgcggtatg caacagcggc gtgcgcgtcc ttcatgcgtt cggcggcgtc tgtgcgtttg
     9841 cgatctgacg gaggcgctgc cggttcatat tctgtggcgt ccacctggta tttagcaagg
     9901 ccaatgcttt tgaggtaatc caccagcgtt tcgaattttt tgaaattgcg aatcgcgcca
     9961 cgcctggccg ctaaaacgcg ctcggtcagt ccgtaatgaa cgaccacacc ccagccgccc
    10021 ggctggccca caatgcttgc gcccttcacg gcccccgctt ccaccaaatg ttcaagggtc
    10081 gtgcggtcga tagtgtcaac agtaatagcc atttcgtcac ctttgtgtca atctctaata
    10141 attcatggta cgccaaaaca atagtaaatg gtaaaaaata ttaaagaaat ttaaccattt
    10201 acttaacata acgcccggta cgtccagcat tggtggctct acttggggtc tgaggtgcag
    10261 tggaacggaa acccaatacg cttgcctaaa atacccgcgc cctgacgtgt gagtggggtc
    10321 agtaatttaa tgttgtcgca acacagcttt gcccaaacca tccagcaact tcaatatgcc
    10381 atccttcatg cccagcaact gagcacaggc gacacgcgct tcatccattt gcccggcatc
    10441 acgcaggcga atgatttcaa cgccagtctg atgcaccagc tgatgcacgg attccaatgc
    10501 tttgaactca gccaagtgcc cgtagcgggc catgccgtga ccgtagtacc aatgacccaa
    10561 gcggcattgg tggtggtcag ttaactgcga tgcagttaac cccaagttgc cgccatccac
    10621 ggccatcacc acacgcttga cccattgaag gtgatcgtac tgcgccacca gcaggggaaa
    10681 gtcactcaac tcccagcgca cgtcagccca ggcaccccag ctgggcgcag gggtgtattc
    10741 caaggcccac gcttgcactt cgctggcggg catgggcttg gcaatgccaa agccctgagc
    10801 cacatcgcag cccaggcgca tcagcagcac gccttgctcg gccaactcca caccttcggc
    10861 caccacattg cggttgaaca cggtagacaa ggtgatgatg gcctcggtca aggccaggtc
    10921 gtccttgtca tccagaatat ctcgaacaaa ggcgcggtca atcttgagcg tttcggtagg
    10981 caggtatttc aggtagctca gtgacgaata accggtgcca aagtcatcca gtgaaaaggt
    11041 cacgcccagg gcctggcagg ccttgatggt gcgctgcacc tgcgcaatgt cccgcatggc
    11101 cacagactcc agcacctcaa tgtccagcca ctgatgcggc acgctggggt ggcgagccag
    11161 cagggcctgc agtcgctgta caaaatcact ttggtgaaaa tgccgcgcgg caatgttcac
    11221 actcactggc cagacatgcc ctgccgcgcc ccagcttgcc aactgagcca gtgcctggtc
    11281 aatgacccat tcaccaatgt caatgatcag gtcagtttct tccaccaagg gcaaaaagga
    11341 cagtggcggc accaaaccat cacgcggatg ctgccaacgc agcagtgcct caaagcccac
    11401 cacctggcca gagcgcatgt tgactttggg ctggtaatgc agcaccagtt catggtgttt
    11461 gagggcgtgc gtcacgcttg ccagtatccg gtaatgcgcc gaggtgtcag tgtcgtgcgc
    11521 tacgtcaaaa aactcaaaac ggttgcgccc ggcttgtttt gctgagtaaa gcgcgtggtc
    11581 agcatggcgc aacagggcgt ctgcgctgac cttgtcgtgc gggtacaggg tgagcccgac
    11641 gctggcagaa atattaaccg tgtcacttgg caaggtgaat ggcatggcca aagccgccag
    11701 cacgcgttcc acaatcggtc gagcgtctgt caaactaccc aattgtgtga gcaccaccac
    11761 aaactcatca ccgcccagtc gtgccactag gtcattggtt tggacagtat ctgtcaagcg
    11821 ttgtgcgact tgtatcagca gttggtcacc gcaggcatgg cccagacggt cattgatggc
    11881 tttgaagccg tccaggtcga caaagcacac gccaatttgc cgcaacccca gatcagcctg
    11941 agtcatggca tgctcaagcc tttgctgcaa cagctggcga ttagccaggc cagtcaacac
    12001 gtcatgctca gcctgaaatg ccaactggtt ttgcaacaga tgacgtgggg tgatgtcaaa
    12061 acggatcgag atgtatttgc gcggcagccc ggtagcttgg tcaagtagcg gcatgatggc
    12121 actggccacc caatacagct gcccgtcttt ggcgcggttg caaatgtcac cccgccacac
    12181 ctggccgctg gtgatggcgc gccacatgtc ttcaaaaaat tccgatgggt ggtggccgga
    12241 attgagtagg cggtggtttt gtcccagcag ctcttgtttg ctgtagtccg agatttcaca
    12301 aaactttggg ttgacgtggg tgatggtgcc tgccagatca gtctcggtgt agatggcaat
    12361 ggcatcgacg gcggtgcggt agttggaagt gtccatttaa atatctgtat cttgaacttg
    12421 gcagactcta aagcccagtg gcctcacgta gtttcttgat ggccacgccc accgcgttgg
    12481 atgcgtttga aaaccccgta ccagccccca gcatggattg cgcctgctca tagcggcctt
    12541 ggttgatgga ggtggccacc cgcccggctt cttggtgaaa gtcggcatgg cggtcaatca
    12601 actgcacaaa ttgcggcaaa tgcccgtaac tggcgcggcc agagccatgc agccatttgc
    12661 caagcacaca gcagtcatca cgcgacaacg tggctgcgtc cagcgtgtca tgcgccgcgc
    12721 tggcgttgcg cagtttgagc ttccagtcgg catgggtttt gattgcgctg tcgagatcaa
    12781 tgttgctttc attgacgctt gagcgtgaca gcagggcagg cgtgcggttg actgcggcgc
    12841 tgctgtcacc gcctggcagt ttgaaaacgc ccagcgcact tgtcaggttg tcggtttgct
    12901 cggacaaatt gccagccgca gcggcagctt cttcaaccag ggctgcgttt tgctgggtca
    12961 cagtgtcaag caggttgact gcctggctca cttgggcaat gcccagactt tgctcacgcg
    13021 tggcggcggt catctcggcc atgatcacgg tcacgcgtgc agtggattca atcacttgct
    13081 gcatggcact gcccgcctgc ccggtaagca aagtgccagc ctctacttta ctcagagagg
    13141 tgtcaatcag caccttgatt tctttggccg cagccgcact gcgcccggcc aaagaccgca
    13201 cctcagcggc caccacggca aagccacgcc cttgctcacc agcgcgggcg gcttctacgg
    13261 cagcattgag cgcgaggatg ttagtttgaa aagctatgcc atcaatcaag ccaatgatgt
    13321 cgacaatttt tttcgaggca gcactgatgt cttgcatggt agtgaccaca tgctgcacga
    13381 tggctccgct tttgttggcg gtttctgacg cgtctaccgc taatgcattc gcttgcgccg
    13441 tattgtgggc gctttgctgc acagtgccgg tcagctgctc catgctggcc gccgtttctt
    13501 ccaggctgct ggcttgagat tcggtgcggt tggacaagtc catgttgccg gtggcaattt
    13561 cttgcgttgc caggttgatg ctttcaaagt tgctgcgcac atcgccaatg atgctgcgca
    13621 ggttgatgtt ggtttggcgc aaggcgtttt gcagttggcc caattcagat tgatcagcag
    13681 gggctatgtc atgtgtcaaa tcgcccccgg ccatggtgcg ggccgcttgg gtgctgtgac
    13741 gcaacggttt ccatactttg ctttgcaagc tcacagcgat gtagcccaca gcccacagcc
    13801 catagcccca agccacaggc agccaataca aaggtgtttg aggtgggcac aatgtctggc
    13861 aacaacacat gcatgtcttc cgctgctggc tttgcctcct ccatcgctgc ttcaatcaat
    13921 tgtttgacgg tggccgcgtc gagtttgtcc agctcgggaa atgcctcgcg ggtgtctgtg
    13981 ctgagcgcta attggtcgga tcgctgctcc cgaatgcttc tgccaacgac aatatttttc
    14041 cggtgtccgt cgcgtcataa ccagccaggg tatctacctc ttcacggaaa gatgctgatt
    14101 gcaggaggcc aatcacttgt tggatcagcg ggtcatccag cgcggagacg ggtagcgcga
    14161 aaaaataacg ctcctgcacc aacggaatga actccagccc gaaacgctgc gctgctgtat
    14221 gcacgccaaa gcccacctcg gccatgccgc tggcaataaa tgcggccacg gccgaatgcg
    14281 tgaattcggt gttttcgaag ccattgatgt cattcggcga aagcgaagcc ccttccagca
    14341 tcagctccag cagcatgcgt gtgccagaac cgacctggcg attgacgaag cgcactccct
    14401 cccgggtcag gtcttgcagg ccgtgaatgc cttggggatt gccggttgcc acgaaaagcc
    14461 cctggctgcg tacggcgaga tgaatcaagc ggtgagtttt tttgttgagc cattgggcat
    14521 acaacgcaac ggcgggcttt tcaaagtcac cgagcggcac gtgaaaaccg gccagatcac
    14581 attcctgacg ggacagcgcg gccacggctt ctgtgctgtt gcggtagcgt agttcaatgg
    14641 gcagtttggc gttgttgatg tcgttgagca aggcggcgac cgcaaaaccg tgactggcat
    14701 gcaggcgcac cgtcctggac ttgcccgccg tcatcttgtt gagttccagc tccaactcgg
    14761 aagacagact ttcgagcatg ggcgacagac gcgccgcaac acggcggtcg gcccaaatca
    14821 atttctccgc caatggcgtg agcactgtac cgcgccccct gcccttgtcc atcagggtgt
    14881 cgccaaacag tttttccaca tcacgcagca agccccaggc gtaacggtag gaaaggccaa
    14941 ttgtttgggc ggcctgcgcg attgatccag tctttttgat ggccaccagc agttccagca
    15001 ggactgccgt gtccaaaggt acgtctgcat ctgaggtgat ttcccagtgc ggttgaatgg
    15061 cgactttata cattatgcaa ataatagctt atttcttctt aaatttttca gtgatatatt
    15121 aagaaaatca aggtaattta ccgatttatt atgtttttaa aagcatattt aattaaacat
    15181 ccacgaatga aaaagcatga attcctcggt catacaaata gtggcaaaga agcccggaaa
    15241 cacgctgcca gcgtttgaca ggcgtgctgt cgaggccatc atcggccagt gcaaggccat
    15301 gccgggtgcc atgctgccta tcctgcacgg ggtgcaggat agagtcggct atatcccgcc
    15361 ggaaacggtg caactgattg ccgatgcgct caacgtctcg cgcgccgaag tgcatggcgt
    15421 catcacctat taccatttct tccgccaaac accgccgggc cggcatgtgg tgcagatctg
    15481 ccgtgccgag gcctgccagg ccgtgggtgg cgaggcgctg gcggtgcatg ccaaggcggt
    15541 gctgggctgt gactttcatg aaacaacgac cgacggcaag ttcacgctgg aagctgtgta
    15601 ttgtttaggc caatgcgctt gcggcccggc cgttatgttc ggcaacgacc tccatgcccg
    15661 agtgtcgtcc ggcaagttcg accgtctgct tgatgagaaa aggagtacgc catgaaaaca
    15721 acggtatatg tgccgtgcga ttcaaccgcg attgcgctgg gcgccaataa tgtggcagac
    15781 gccatcgccc gtgaggcgca ggcgcgcggg atagatattc gcatcgtgcg caacggctcc
    15841 cgcggaatgt tctggctgga acccctgatc gaagtcgcca ccccacaggg tcgtatggcc
    15901 tatggcccgg tggaggtgga ggcggtggcc agcctgttcg actcgggctt tgccctgggc
    15961 gcgatccatc cgctcgcact aggtttgacc gatgagattg ccttcctgaa aaatcaggaa
    16021 cggctgacat ttgcgcgtgt tggcatcatc gacccgctct cgctggacga ctacttggcg
    16081 cacgacggtt atgccggact gcagcgtgcc ataggcatgg cgcaagcgga catcgtccaa
    16141 gaggtgctgg actccgggct acgcggccgc ggcggcgcgg ccttcccgac cggcattaag
    16201 tggaaaaccg tgctcggtgc gcaggccgcg caaaaataca ttgtctgcaa cgccgacgaa
    16261 ggcgattccg gcacgttttc tgaccgcatg gtgatggagg acgacccctt catgctgcta
    16321 gaaggcatga cgattgccgg cctggccgtc ggcgcgaccc agggttatgt ctacgtccgc
    16381 tccgaatacc cgcatgccat cgcggctctg accgaggcga ttctcatcgc gcgcgagaat
    16441 ggttatttgg ggcccaatgt cctggattcc ggccaggcct ttgaactcga agtgcgcaag
    16501 gcggcaggcg cctacgtctg cggcgaggaa accgcgctgc tcgaaagcct ggaaggcaag
    16561 cgcggtatca tccgcgccaa gccgccgctt cctgcgctgg aaggcctgtt cggcaaaccg
    16621 accgtcatca acaatgtcat ttcgctcgct gccgtgccgc tgattctggc gcgcggcgca
    16681 gctttctacc agaactacgg aatgggccgc tcacgtggca ccctgccttt ccagattgcc
    16741 ggcaacatca aacatggcgg cctggtcgag aaagcctttg gcctgaccct gcgtgaactg
    16801 ttgatcgact tcggcggcgg caccgccagc ggccggccca tccgcgccgt gcaggtcggt
    16861 ggtccgctgg gcgcctatct accggaatcg ttgttcgaca cgccgatgga ttacgagtcc
    16921 ttcatcgcca ttggcgcagg tgtggggcat ggcggcctag ttgtgtttga cgacagcgtc
    16981 gacatggcca aacaggcgag gtatgcgatg gagttctgcg cgatagagtc ctgcggcaaa
    17041 tgcacgccct gccgcatcgg ctcgacgcgc ggcgtagaag cggtcgacaa gatcatcgcc
    17101 aacgagaacc ggccgcaaca gattcacttg ctacgcgact tgtgcgacct gatgctcaac
    17161 ggctcgctgt gcgccatggg cggcatgacg cccttcccgg tgctgtccgc gctgaaccac
    17221 ttttctgaag atttcggcag cactcccctg caaaaagctg cctgaaccca ctcacaagaa
    17281 caagcgaagg attccccatg aaccagctca atgaactcga ttacggcaca ccggcgcgtg
    17341 tatcggaaaa gaccatcacc atcgaaatcg atggtgtgca ggtcaacgtc ccagccggca
    17401 cgtcggtgat gcgcgcggca gtggacgccg gtgtcaacgt gccaaaattg tgcgccaccg
    17461 acagcctgga acccttcggc tcctgccgcc tgtgcctcgt ggagatcgag gggcgccgcg
    17521 gctacccggc ctcctgcacc acgccttgcg agcccggcat gaaggtcagg acccagacgc
    17581 ccaaactggc tgaaatccgc aggggcatga tggagctcta catatccgac cacccgctgg
    17641 actgcctgac ctgccccacc aacggcaact gcgaactgca ggacatggcc ggcgtggtcg
    17701 gcctacgcga cgtgcgttat ggctacgatg gcaagaatca cctgaagctg gcgaaggacg
    17761 aatccaaccc ctacttcacc tatgacgcgt ccaagtgcat cgtctgcaac cgctgcgttc
    17821 gcgcctgcga ggaaacccag ggcaccttcg cgctgacgat cagcgggcgc ggcttcgact
    17881 cacgtgtgtc agccggccag gaccagagct tcatggagtc cgaatgtgtt tcctgcggcg
    17941 cctgtgtcga tgcctgcccg accgcgaccc tgaccgaaaa aagcgtcatc aagatggggc
    18001 aggccgaaca cagcaagatc accacctgtg cctattgcgg cgtcggctgc tccttcaagg
    18061 ccgaaatgaa aggcaacgaa gtcgtgcgca tgattccgaa caagaatggc catgccaacc
    18121 atggtcactc ctgcgtcaaa ggccgttttg catggggcta cgcaacgcac agggaccgca
    18181 tgcttaagcc catgatccgc aaggctatca ccgacccatg gcaggaggtg tcctgggacg
    18241 aagcggtcaa ttacgccgcg tcggagtttc gccgcatcca ggccaagttc ggcaaggact
    18301 cgataggagg catcacctcg tcgcgctgca ccaacgagga aacttatctt gtgcaaaagc
    18361 tggtgcgcgc cgccttcggc aacaacaacg tggacacctg cgcccgcgtc tgccactcgc
    18421 ccaccggcta tggcctgaag cagaccctag gcgaagcggc cggcacccag actttcaact
    18481 ccgtgatgaa ggccgacgtc atcatgatca taggcgccaa cccagccgca gcgcacccgg
    18541 tgtttgcttc tcagatgaaa cgccgcctgc gccagggcgc taagctaatc gtgatcgacc
    18601 cgcgtacaac cgaaatggtc aagtcgccgc acatcgcggc cgacttccat ttgaaattga
    18661 tgccggggac taacgtggcc gtcatcaccg cactggccca tgtgatcatg agcgaaggcc
    18721 tatttgacgc agccttcatc gccgaacgct gcgaagagca gtcctttcag gactggcaga
    18781 aattcgtgct gcaacccgac aactcgccgg aagccgtggc cgccatcacc ggcgtatctt
    18841 ccgaagaaat acgcggcgcg gcgcgactgt ttgccgccgg caagaatggc gcgatttatt
    18901 acggccttgg cgtcaccgaa cattcgcaag gctcaactat ggtgatgggt attgccaact
    18961 tggccatggc caccggcaat atcggccgcg aaggcgtggg cgtcaacccc ctacgcggcc
    19021 agaacaatgt tcagggcgct tgcgacatgg gttctttccc gcatgagttg ccgggctacc
    19081 gccacatctc cgacacaacg gtgcgtaccg aatttgaaga agcctggcaa tgcacgctgg
    19141 atccggagcc aggcttgcgc attcccaaca tgttcgacgc ggcagtcgac ggcaccttca
    19201 tgggcctgta ttgcgaaggc gaagacattg tccagtccga cccgaatacg caacacgtca
    19261 ccgctgccct agctgcgatg gaatgtattg tggtgcagga cctgttcctc aacgagactg
    19321 ccaaatacgc ccatgtcttc ctgccaggat cgtccttcct ggaaaaagat ggtaccttca
    19381 ccaacgccga acgccgcatc tcacgcgtgc gcaaggtcat gccgcccaaa gccggcaagt
    19441 ccgactggga agtcaccgtg gccttggcca atgccttggg ctatcccatg aattaccaaa
    19501 atccctcgga gatcatggcg gaaattgccg cgctgacacc aacgttccgt ggcgtcagct
    19561 accaaaagct cgacgagttg ggcagcatcc agtggccctg caacgatgca gcgcccgaag
    19621 gaacgccgac catgcatatc ggtgaattcg tgcgcggcaa gggtaagttc ataaacacgc
    19681 agttcttcgc cagcgctgaa aaggtctcgc gcaaattccc gctgatcctg accaccggcc
    19741 gcatcctgtc gcagtacaac gtgggggccc aaacccgccg cactgacaac gtgcaatggc
    19801 atagcgagga ccggctcgaa gtccatccgt acgacgcgga agaacgcggc atcaagcaag
    19861 acgactgggt cggcatcgaa tcgcgcgccg ggcaaactgt gctgcgcgcc accattacaa
    19921 accgggtgca gcccggcgtc gtgtacacca ccttccactt cccggagtcg ggcgccaatg
    19981 tgatcacaac caacaactcc gactgggcca ccaattgccc ggaatacaag gtcacggcgg
    20041 tgcaggttat gccggtcacg cagccgtcgg aatggcagag aaaatacaac cgtttctcga
    20101 gcgaacaagt cggccttgcc acaggtgcag tcacggcagg cgagcaaagt ctcgccgtgg
    20161 tcggcaagta aacggggaaa tgcgagattt tatgaatgca ataacgtccc cagaacgagc
    20221 gacgctggta caggtctccg tggaaaagtg gcgtaatgac cagcgtagac tgtgcctgaa
    20281 cacgctagca gaggaagtgc ctattgcgct tgaatacaac ggcatttccc acgcggtgat
    20341 gctggcctcc cccttcaacc tggaagactt tgcgctgggt ttttccctta gcgaaggcat
    20401 cctggccgat tcctcggagc tgttcgactg cgaggtttcg gcaacgaaaa atggtatcca
    20461 ggtgcagatg caaatcgcca gcgggcggtt tgccgagctc aagagcaaac ggcgcaattt
    20521 gacaggccgt accggttgtg gcttgtgcgg cgccgagacg ctcgagcaag ccatccggcg
    20581 cccgccggtt gtcaacagta ctgcgctttt ttcagccgaa gcgctgtatg ctggcatgga
    20641 cgctatgcag atgcatcagc acttgcaaca tcaaacagga gccacgcacg ctgcggcctg
    20701 gcttggttcg gatggccgac ttgaactggt ccgcgaggat gtcgggcggc acaacgcgct
    20761 ggacaaattg ataggcgcca tggcttcgga gaagcgaagt ttctctgcgg gtgctgcatt
    20821 gatcaccagc cgcgcgagct acgagatggt gcaaaaagcg gccaccttgg gcatcggctt
    20881 cctggctgcc atttccgcgc ctaccagtct ggcggtgcag ttggccaagg aagctcatgt
    20941 aaccttgctc ggttttgtgc gccaccaaag ccatgtggtt tacacccagc cactgcgcct
    21001 ggaatgtgga aaacttgaat caaggagtgt gtcatgaacg cagacaacct cgttatcatg
    21061 gcgaaccaga tcggctcgtt ttttgaaact atgccggaca ggcaacaggc cttgacggat
    21121 attgccgggc atattaaacg tttctgggaa ccacgtatgc ggcgatccct gctgaagcac
    21181 gtggatgaat atgccggcgt acagctcaag gacattgtcc tggaagccct tcgcgtgcac
    21241 cgggcgtcga tcctgtaagt gccccgcagg aaacgaggca ctccgagtgc tgcattctga
    21301 ttgaggtgtc gactagcaaa tcccatcagg gccagcaggc aagtcatcct gccccgaaag
    21361 gctgaatgta cgggtgtaac ctttatctta acgctgtcat gaattgagga cttggcaaac
    21421 aaggggttca tgtccgtttc ctgagacggc ccccatgttt actcggggga agaattcatc
    21481 gaccaaggac agcaacgcta cgatgaacag cagcttacgc gtagcgtcgt ggcactcaga
    21541 caccgagcca gcacactgag ctttacgatc actgcggtgg tggccgaggc catgaaaacc
    21601 cgattcaggg gagttttttt cttaagagag gccaaaagca aggtcatgaa tttcacattt
    21661 aaacaactcg aatattttgt agcggtcgca gactgcggga caatcaccgg ggcggcagaa
    21721 aaaatccatg tctccgcgcc ttccatttcc gtagccattg cccaacttga agaatctttg
    21781 gaaatacgat tatttacccg gcaatcgagt ggcttacaac tgacgaccgc gggcgatgaa
    21841 gtacggcgac gcgttcgaac aatactcgaa gaaactcgcg gcctgcaaga cataagtaac
    21901 actgtgactg gtgaactgcg aggaaagatt tcactaggat gcctgaccac gttggcccca
    21961 ctccttctgc cggaggtttg ccagacgttc tcgcgtatcc atcctgccgt ttcggtggag
    22021 cccattgacg gaagccaaga tcagctgatc ggataccttc ggcgcgcagt cattgacctc
    22081 gtgattacgt acgatatgca tatcccacaa gacctgtggt ttgaatccct tgtagggcgt
    22141 caccccctga tcatgttgag ccctgatcac agactcgccc aggcaccatc tgtcgagatg
    22201 aaagacttgg agagcgaccc gtacattctg ctagatctgc ccatcagccg ggactatttt
    22261 ctatcgattt tcgaaaaaac cggcatcacg ccagacatca aaatgaggac aacgaatttc
    22321 gacgtcgtcc gaaccatggt cgccaatggg tacggcgtct ctatcagcgt ggtacacccg
    22381 aaaaacgagg ccgcactgga tggcaaaccg atcatttaca agcctatctc aaacccactg
    22441 ccgctgctca atatagggac actatccaga cgaaccgatt tcaccagcat tgcgacatca
    22501 ctgaaaaggc acatacagag cctcattacg tcaagcgcca tccctgggat gcgcctgatt
    22561 gaccatcacc aagttccgac gttcggcata tctacccaag gcttccgtgg tggcaagggg
    22621 tt
//
LOCUS       DAOSLL010000004        22575 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_2546_length_22575_cov_4.719005, whole genome shotgun sequence.
ACCESSION   DAOSLL010000004 DAOSLL010000000
VERSION     DAOSLL010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 22575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22575
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2546_length_22575_cov_4.719005"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1439)
                     /locus_tag="PLB25_00320"
     CDS             complement(<1..1439)
                     /locus_tag="PLB25_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HOE40072.1"
                     /translation="MMDLLLANGHGEDVEDIYALSPLQEGMLFHVLREPDAGMYFQQA
                     AFTMTGLAIPAFERAWQRLVDRHPILRTSFHWEGREKPVQVVHRNLVMKVERLDWRAF
                     PPAEQEAHLTEFLREDRQRGLPLDQAPVQRITLIQIADNAHHYVWSHHHILLDGWSGP
                     LLFKELFGFYEAFRRGGDLNLPKPRPFADYIAWLGRQDMEKAEAFWRDCLKGLSAPTP
                     LPEDRSGEPRHLRGDRFEQRHVQLSETVTTALNGLVRRQQITLNTLVQGAWALLLSQY
                     CDLDDVCFGTLVSGRTPELDGIEHMVGLFINTLPVRVRVPQDAALIPWLKGIHAQQSE
                     MRQYEYSPLVQVQGWSEIKRSLPMFESLVVFENYPGGKDPSQAGRGQSGSKFKRTRSP
                     KERTNYPLAILAGPGKELLLRATFDRTRFDPDAIGRILEHLAVICNAIAADPDRHLGD
                     LGILSPAERQRVLVDWNETRVDFGGPECA"
     gene            complement(2344..5154)
                     /locus_tag="PLB25_00325"
     CDS             complement(2344..5154)
                     /locus_tag="PLB25_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOE40073.1"
                     /translation="MLELLKTFSWQELRQHPWRNAAAVLAVMLGVALAFSVHLINASA
                     LDEFAQATRSVAGQSDLELRAVQGHFDEALYQRVAQMPGVMLASPVLEVGSYMATAQG
                     KRSLRVIGIDALTVLGVSPDLMPLPEPGQATLEATDQVPGNQPTGPARAGRWVLFAPG
                     QVFLNAVARRLLVNSPPGTVIPSPASVPVKAAPARPAVSPNPAFTTQQTMPATPAGKA
                     SGSLPANASDLPFAAPGLQLQSGMGWLTVNVAGSVRAGGSALAVMDIGAAQELFGKQG
                     QLSRIDLRLQPGTDRDAFAKALQTRPDWPGDVTVAAAEDARSQISNLSRAYRVNLTVL
                     ALIALFTGAFLVFSVLSLSVAKRAQQFALLGVLGLSGRARLALVMWESLALGLVGSAA
                     GLALGSGLAALALRLLGGDLGGGFFGGAAPTLQFSAAAAAVYGSLGVAAALAGGWWPA
                     RTAGQLPPAQTLKGLGSTTSRSKSHWVSLVLIAVGAMLTSAPAVFGIPLAAYLSVGLL
                     LLGGITALPWLIGLLLGYLAPLLARQTLPLLAVERSRRQRDTAAVAVSGIVASLSLAV
                     ALTVMVASFRESVTQWLDRVLPADLYVRSAGNLGASDTVFFSPEFVAAAAQVPGVNKL
                     QAMRQQPVMLQSSLPAVALIARELGDPARSLPLIDTPLPVPAGQTGIYVSEAVVDLHG
                     ARPGQSFPLFSKAFRPLALINKAPPATFFVAGVWRDYARQSGAIAIDRRAFIDLTGDL
                     RANDLALWLNHPADATRIAQALLVLADPAAATPTRGDGTGGLLEFGTAAQIRAISLKV
                     FDRSFAVTYWLQAVAIGIGLFGVAASFSAQVLSRRKEFGLLAHLGLTRRQILTLVALE
                     GAAWTLLGALAGLGLGLLVSVVLVHVVNPQSFHWTMDLLLPWPRLLLLCLAVVLAGTV
                     TAWLAARKAAGKDAVLAVKEDW"
     gene            complement(5549..6478)
                     /locus_tag="PLB25_00330"
     CDS             complement(5549..6478)
                     /locus_tag="PLB25_00330"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005670689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE40074.1"
                     /translation="MNPSFTLLIVDDEKHNRTLLTELLQDDYQIILAKNGVQALEKAR
                     ERSPDLILLDVLMPEMDGLAVIRALKAGDGTRHIPVIFVSALDSTLDEERGLELGAVD
                     YITKPFHPPIVRVRVRNHLQSVHQRRLLEQLAMIDSLTEIPNRRRYTEVYKLEWRRCL
                     RNSRPLSLIIVDVDHFKIFNDTYGHAAGDLVLKRVAKAIQSALKRPADFVARYGGEEF
                     VVVLPEIDAGFAQQVAEAIRAAVQALAIAFPESATSPWVTVSLGGASWLPRHNEVDTE
                     LFGCADHCLYDAKRSGRNRVQWDSGNGVSPSET"
     gene            complement(6475..9540)
                     /locus_tag="PLB25_00335"
     CDS             complement(6475..9540)
                     /locus_tag="PLB25_00335"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE40075.1"
                     /translation="MSQRTLAQWLHPHSVRGKLVLLSCASLLLAVLLVFALLVLQQQR
                     LIHNDWASSLATQARLVAISSQAAVAFEDRVEATRLLAAFKSNPSILRARLMDQNGQV
                     FAQYQSSQPDPLLLVMPPALANSQVADDVMTVWAAVPGDASSPAVVELSASLKEMKAA
                     LLQTALESGLILLAALGMSLWLSVRLVRRLASPLEELSQLMTAVSTDATLPTRFHRQG
                     DDEVALLGKGFNAMIDTLQGRDLELQESQQNLEQLVAQRTHQLTLATDEANQANQAKS
                     DFLARMSHEIRTPMNAIIGLGRLLLKTRLDAQQRDYQEKVLTSSDALLGVINDVLDYS
                     RIEAGKLTLESIPFNLNQVMHNLSNLMAVKAQEKGLELLMLIDEAVPRHLLGDPMRLG
                     QVLTNLVNNAVKFTQAGDVVVRVKRADERSAPARPGEQVELAFSVSDTGIGIPHERLA
                     DLFCPFTQVDGSISRRFGGSGLGLAICKQLTELMGGHIGVESMVGRGSRFHCTACFTV
                     TDNQLPTAVHSHHLAGKHVLVIDDNHNARLALCQMLQHYGMRAEAASGGQQGLDMLSA
                     AATAGDPFALVLLDWLMPDLDGLQTAQRMQDNAAALGGAPALLMITAGSYDKIMDKLA
                     AVGLNRVLSKPVSESLLHDAMLEVLMGAPLARAHQQNRDLERERQYDFSGIRQARVLL
                     VDDVEINRSVALAFLQQAGIKAEIAVNGEEAVAKVNQNDYDLVLMDIQMPVLDGLAAT
                     RAIRSNPAHDCLPIVAMTAHAMSTDRDRSLAAGMNDHLTKPIDSDALFTALLRWITPR
                     KAGPDGATPTGAGVPDGGYEPALPPLDGIDTGRGLINHMHRPALYRQMLAGFQREFGS
                     TADDIDSALAQGDFGVALRLAHSMKSAAATIGAVELSQCAKALEQRYEQKLRADAEFP
                     VFVLALRRVLQSLSALPDVQHLSDNGAGPASAASFDVQLGLIERLKTLLRSDDAAAGR
                     LCDELKICLNDPRQQNDVILLRDLIDDVEYQEALIVLARLQATLSTLRP"
     gene            complement(9537..10169)
                     /locus_tag="PLB25_00340"
     CDS             complement(9537..10169)
                     /locus_tag="PLB25_00340"
                     /inference="COORDINATES: protein motif:HMM:NF025069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HOE40076.1"
                     /translation="MTRLRALMLWLWPSTACRVSAVGPGDPALRQAPGQIALLMMATQ
                     GAAQSRGVAVSESALKSALKSALKSALFFKLPQFVYRPNGAPDRPFSFCLPGSNVFNA
                     AFETLVPIDGRGVTLSKLGAAAQTSRCGLVFVSQSGASGLAAVLRHLTTQPMVTVSDI
                     AGFARAGGMGELTPAGGGAPVNILINRKAARLQDIAFNAQLLRLARVVEP"
     gene            complement(10166..10366)
                     /locus_tag="PLB25_00345"
     CDS             complement(10166..10366)
                     /locus_tag="PLB25_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40077.1"
                     /translation="MTGMKSENGLAQSVGGHWQPSDQAPLDQLCPADFQRKSATTLGG
                     TVVPAAQKLDGPACVSTLKALS"
     gene            complement(10363..10845)
                     /locus_tag="PLB25_00350"
     CDS             complement(10363..10845)
                     /locus_tag="PLB25_00350"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOE40078.1"
                     /translation="MDLINIPVVTASRQLETLDRMSSCILVFTRAQVREHRYRNPTDL
                     RQDLPDFDFKRGTRSSACRHFTVQGLVGPNKLLMDGAHIGNPAGGSLPVAENIALHPA
                     KQVELLFGLAAAVCGADALAWGSTSSPARRTVPAQTGRHPAVARVACRVSRVARRLPA
                     "
     gene            complement(11096..13000)
                     /gene="pabB"
                     /locus_tag="PLB25_00355"
     CDS             complement(11096..13000)
                     /gene="pabB"
                     /locus_tag="PLB25_00355"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HOE40079.1"
                     /translation="MQIRLDFCDPRASIAAPTDQASAAGTNPGAGALRCAFDSPLQTL
                     IADTADQVKPLLDAVQALAKQGRWCVGYLRYEAAGAFDSAYVTHNVDAPLAWFGVFEQ
                     ALPWPESECVDPADPADQAPKTADETPIDWQCALSRPEFDAQMASIAQAIAAGECYQV
                     NYTAPLKARFADSAAGLFERLQRAQPGGYAALIDTGSEQVLSVSPELFFDWQDGQILT
                     RPMKGTAPRGATASADAALAAGLTTSPKERAENVMIVDLLRNDLSRIALPHSVKVPAL
                     FRVQSLPTVHQMVSDVTAATRPGTTLADVFTALFPCGSVTGAPKVRAMQMIRALEPQP
                     RGIYCGAIGVVRPGGHATFSVAIRTVCLHGETATCGIGSGITFDATADDEWREWQHKR
                     GFLERASAAFGLLETLRLEGRKFHNLEAHLARLQGAAAHFGYPFDELRIRQTLTRVAS
                     SSEAVQGDRHGHTRRLTAAMGQRAKALAQHQPKVWRVRLQLDPKGQPTVRIFIHKSTP
                     EPVSIALAAIPFEASQSEFTRFKTTHRAHYEAFAPTGAGIFDTLLYNPQNELTECTRG
                     NVALLLDGQWLTPALHCGLLAGVGRARCLSEGVVQEAVITLGDLPRVQALAFINSLRG
                     WLDARLVQPR"
     gene            complement(12984..13646)
                     /locus_tag="PLB25_00360"
     CDS             complement(12984..13646)
                     /locus_tag="PLB25_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE40080.1"
                     /translation="MSLVISHLGKRYGEVPVFKHVSLRVAAGEFVAIMGESGVGKSTL
                     LNCMAGLDTLNHGSVLLDGQDLSRQSDDQLARLRREKTGFVFQAFHVLPHLSVAQNVA
                     LPLMLLNQHNNARVLAMLEAVGLQDLGERLPQQLSGGQLQRVAIARALVHRPMLVLAD
                     EPTGNLDPRTAERVMDALVSQTRAHGAAMVLVTHSKAAAARADRVLLLTAEGLTGHAD
                     QA"
     gene            complement(13643..14623)
                     /gene="miaA"
                     /locus_tag="PLB25_00365"
     CDS             complement(13643..14623)
                     /gene="miaA"
                     /locus_tag="PLB25_00365"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HOE40081.1"
                     /translation="MPRYLALAGPTAAGKTAAALAIAAQHRVEIISVDSALVYRGMDI
                     GTAKPDAAELAAVPHHLINIRDPLHAYSAAEFVADASALMHDITERGKLPLLVGGTML
                     YFKALQDGLDDLPRADPAIRQALEQEAAQKGWPALHAELALVDPVTAARLAPADAQRI
                     ARALEVYRITGLPMASFHTIKNVACSAISTGATGQNDHENMRHPSLLISLEPSDRAWL
                     HQRIAQRFDAMLRDGFIDEMLALRSRGDLHPNLPAMRCVGYRQAWEALDGLWPLKDLR
                     DKGIFATRQLAKRQITWLRANRQRQVVACDAPDAVAQVLTLARAFICNPA"
     gene            complement(14957..16795)
                     /gene="mnmC"
                     /locus_tag="PLB25_00370"
     CDS             complement(14957..16795)
                     /gene="mnmC"
                     /locus_tag="PLB25_00370"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR03197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HOE40082.1"
                     /translation="MPAPQAGVVQADQAAAKLWQGSTSWRVLDTDFHHGRAFFETWLA
                     WQRDPDRPRLLHYVALCPLAPSPQSLTDRCADEPDLQALAASLAKQWFGILPGFHRFL
                     LADGQVTLTLCAGDAMHLLRQQQFHADWVTLRLAADKPPGALEAQSLWSIKALARCCR
                     RGTRLQCLGAQPTDLAALSSHLKQCGFDLASGPAHQAGIAAFQLTAVFHPSWTLKSAR
                     QPGIAQALPVQRCAVIGAGLAGASVAASLARRGWQVLVLDQGADAAAGASGLPVGLVV
                     PHVSSDDCSLSRLSRAGVRMMLQQADQHLQAFQQWAPSGVLERQIDGTPELPAQWPDA
                     GKAWCMQASAMPAGATTATLGPSLWHAKAAWIKPAELVRAWLRQPGVSFQTNARVSSL
                     RFDAGVWQLLDATGAVLCVAERVVLANACGARDLLQALASQSLELAQRLKDLPATQGM
                     RGLLSWARHADSASATPHFAPCPVNGSGSMISQVPTQDGQAWFAGSSYQPDSGAERAD
                     QDNHASNLTHLQKLLPALASELAPMFASNALNTWKSTRCVSSDRMPLVGPLDAGRQPG
                     LWICAGLGSRGLSFSVLCAELLAARMGAEPLPVEANLANALEALRG"
     gene            complement(16800..17072)
                     /locus_tag="PLB25_00375"
     CDS             complement(16800..17072)
                     /locus_tag="PLB25_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HOE40083.1"
                     /translation="MARTVNCIKLGVEAEGLDFPPYPGELGKRIWQSVSKEAWGQWLK
                     HQTMLVNENRLNLADARARQYLARQMENHFFGGGAEAAQGYVPPKG"
     gene            17157..17387
                     /locus_tag="PLB25_00380"
     CDS             17157..17387
                     /locus_tag="PLB25_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40084.1"
                     /translation="MAKADTKTKIVADGLRYKSKVSGSPFTAATSFGAATMSCFLCGK
                     HRARSQMVSKKILGKSQAVCSPSCKALDEANK"
     gene            17400..17912
                     /locus_tag="PLB25_00385"
     CDS             17400..17912
                     /locus_tag="PLB25_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HOE40085.1"
                     /translation="MLCSLPDGALRRVYLALSAVCFGMLAFGLYLQHSVGLDPCPMCI
                     VQRYALLFVAIIALLAGASGKKMLHLLGAALLLLGAGFGAFVAARQTWLQWYPPEIAT
                     CGRDFYGMIESFPLNRAIPMIFRGSGDCSKVDWTFLGGSLANWSFVSFTLIGVLALLI
                     LWRNATQRVR"
     gene            complement(17919..19028)
                     /gene="ppk2"
                     /locus_tag="PLB25_00390"
     CDS             complement(17919..19028)
                     /gene="ppk2"
                     /locus_tag="PLB25_00390"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005672734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HOE40086.1"
                     /translation="MAVRKPLASVVRRTVLGDTSGAKTTAGVARHAVQASLDAAQGSQ
                     QTAIRDILANAPDKADALKTLLAGSSSDDIAAIRLMLLGGQLDPHGKAMAPDEELSLD
                     WRSGGYPYKNLLSRKRYESDKYKLQVELLKLQSWAKETGQRVVILFEGRDAAGKGGTI
                     RRFMEHLNPRGARVVALEKPTESERGQWYFQRYVQHLPTAGEIVLFDRSWYNRAGVEQ
                     VMGFCSEAEYQQFIHQAPLFERQLVQSGVYLIKFWFSVSRKEQRRRFKERELHPLKQW
                     KLSPIDLASLDKWDDYTKAKEAMFFETDTADAPWTVIKSDCKKRARLNAMRFVLHKLP
                     YANKTSSNIGSLDTLLVGRAHVVYERGEHPSSALL"
     gene            complement(19344..20048)
                     /locus_tag="PLB25_00395"
     CDS             complement(19344..20048)
                     /locus_tag="PLB25_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HOE40087.1"
                     /translation="MSSLNFIGGEKGGVGKSVVARVLAQYFIDKQLPFVGFDTDRSHT
                     SFTRFYADYASPVLVDSYEGLDQVVSVFEGEADAAIKPRVIVDLAAQTAAPLARWIND
                     SELLALLADMGIAVNFWHIADAGNDSVELLDHLLDTYGEGPNYLVVKNLGRGSDFSQL
                     EESAAMVKARAFGARTITLAQLHEGSMRKIDRQNVSFWAAVNSRPDVDALGLLERQRV
                     KSWLKRTYESFDALPL"
     gene            complement(20142..21491)
                     /gene="argA"
                     /locus_tag="PLB25_00400"
     CDS             complement(20142..21491)
                     /gene="argA"
                     /locus_tag="PLB25_00400"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HOE40088.1"
                     /translation="MSLPFNFTFVPWFRSVAPYIHKFRNQTFVLGVCGEAIAAGKLPN
                     LAQDLALIQSMGVRLVLVHGFRPQVNEQLTAKGHAARYFKGIRVTDEVALDCAQEAAG
                     QLRYEIEAAFSQGLPNTPMAGATVRVISGNFLTARPVGIVDGVDFLHSGVVRKIDTAG
                     ITNSLSMGALVLISPFGFSPTGEAFNLTMEEVATSVATALQADKLIFVTEVPGIATDV
                     RQAVSEENPIITELPLAAAEKLLADLPGADKPGDTACYLQHCVKACKGGVERSHIIPF
                     AVDGSILLEVYVHDGIGTMVVDEKLESLREASVDDVGGILKLIEPFEKDGTLVKRNRT
                     EIERDVGLYTVIEHDGVIFACAALYAYPDARTAELAALTVSPDVQGQGDGERVLKRIE
                     QRAKLAGLDSIFVLTTRTMHWFIKRGFEQADPDWLPEARKRKYNWDRKSQVLVKKIN"
     gene            complement(21978..22067)
                     /locus_tag="PLB25_00405"
     tRNA            complement(21978..22067)
                     /locus_tag="PLB25_00405"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22031..22033),aa:Ser,seq:gga)
     gene            complement(22176..>22575)
                     /locus_tag="PLB25_00410"
     CDS             complement(22176..>22575)
                     /locus_tag="PLB25_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE40089.1"
                     /translation="MSNIKYFGGDGICTNELIKIAAGAKTLDGVVCAEGGSSIAKMPG
                     GTAWKAKYDSKYPGQFQIYSPYTYDATFVLVDAMKRANSTDPKVYTPKLIETKFKGVT
                     AEIAFEPSGEMKNPTMTLSTYKAGKKIALN"
BASE COUNT         4427 a         6909 c         6799 g         4440 t
ORIGIN      
        1 tgggcgcatt ccggaccacc aaaatcaacc cgggtctcgt tccagtccac caggacgcgc
       61 tgccgctccg ccggtgacag gatgccgagg tcgcccagat gccggtcagg atccgcggca
      121 atcgcattgc agatcactgc cagatgctca aggatgcgtc cgattgcgtc cgggtcgaag
      181 cgtgtcctgt cgaaagtcgc gcgcagcagc agttctttgc ccggcccggc aaggattgcc
      241 agggggtaat tggtacgttc ctttggggag cgggtgcgct tgaacttgct gccactctga
      301 ccacgccccg cctggctggg atccttgccc cccggatagt tctcgaacac aaccaggctt
      361 tcgaacatcg gcaggctgcg tttgatttcg ctccagcctt gcacctggac cagcgggctg
      421 tattcgtact ggcgcatctc tgactgctgg gcatggattc ccttgagcca gggaataagc
      481 gccgcgtcct gcggcacccg cacccgcacc ggcaaggtat tgatgaacaa ccccaccatg
      541 tgttcgattc catcgagctc cggagttcgc ccggaaacca aggtgccaaa gcagacatcg
      601 tcaaggtcac agtactgact caggagcaaa gcccaggcac cttgcaccaa tgtgttaagg
      661 gtgatttgct ggcgccgcac caatccattc agggcagtgg tgacggtttc ggagagctgg
      721 acgtggcgct gttcaaagcg gtccccgcga aggtgccgcg gctccccgct gcgatcctct
      781 ggcagtgggg ttggcgcgga taggcccttg aggcaatcgc gccagaaggc ttcggctttc
      841 tccatgtcct gccgccccaa ccaggcgatg tagtcagcga atggccgcgg cttgggcagg
      901 ttcaggtcgc cgccgcggcg gaaggcttca tagaagccga acaactcctt gaacagcaag
      961 gggccagacc agccatcaag caggatgtga tggtgactcc agacgtagtg gtgggcgttg
     1021 tcggcgatct ggatcagggt aatgcgctgc accggggcct ggtcgagggg cagaccgcgc
     1081 tggcggtcct cgcgcaggaa ctcggtcagg tgcgcctcct gttctgcagg cggaaaggcc
     1141 cgccagtcca ggcgctccac cttcatgacc agattgcgat ggacaacctg gacgggtttc
     1201 tctcgcccct cccaatgaaa ggatgtacga aggatcgggt ggcggtcaac caggcgctgc
     1261 caggcgcgct cgaacgcggg gatagccaac ccggtcatgg tgaacgcggc ctgctggaag
     1321 tacatgcccg cgtctggctc gcgcagtacg tggaacaaca tcccctcttg tagcggtgag
     1381 agtgcgtaga tgtcttcgac atcctctcca tgtccgttcg ccaggagcag atccatcatt
     1441 aatatttcct attttctatt ccgccacgaa gtcaatgaaa ataccgatca gcggatgttg
     1501 gcaggcatta tcgccggact ggcaatcgat actgaaaccc cggcagcctg tcggactcac
     1561 ataccatttc aaaaccacaa ccagggtttt cacaaagatg aattttggcg gacattgctt
     1621 gatttgcagg caaattgcct gattttcata gcatcaaatt cataggaatc aattacttaa
     1681 agggcagata gcctcctgtc agcccatcgg acttatcaag cgtttaaaaa caacagcttg
     1741 cattttcaag cggatgaatc tttgcagata ttccgcacta tttaagcaaa ctacctgact
     1801 ttcatggctt gggagctata tgaatcaacc acttaaaagt ccgaccgtct cctggcctgg
     1861 tccaaccagg tgtgcgcctg aatggctagc gaatttccac ccagaagtgg actcatggcg
     1921 gaatggcgtt gttcaatgca tcaatggcct gtggcggttc aggtttgcga ctggtgcgcc
     1981 caccctccga acttctcagg ccccttgcat ccgacattgg cggctacagg aattttgact
     2041 gcaccagcgc cgactgcagg tcatcttggt ttgcttgcgg tgatctctgc tgcgcccggg
     2101 caggccagat ctgtccattt atttgcgctt tggtcactgg cattggcagg gaccaagcaa
     2161 gtcggcctgc caccagttgg ccccggcgac caggcagcgt caagccagtg ccatgaattc
     2221 gaacgctttg cccggccggg accagtcggc aggaatctgc ttttggaagt ggtgcaatgg
     2281 cttgccgcaa gcaagcgcaa gaaaagacaa aacaaacaaa gcgctggtcg agaaaaatgg
     2341 tcgctaccag tcttccttga cggccagtac cgcgtctttg ccggctgcct tgcgcgctgc
     2401 cagccaggcc gtgacggtgc cggccagcac cactgccaga cacagcagca gcaggcgtgg
     2461 ccagggcagc agcaggtcca tggtccagtg aaaactctgc gggttcacca catgcaccag
     2521 caccacggac accagcagcc ccaggcccag cccggccagc gcaccgagca gggtccaggc
     2581 ggcaccttcc agggccacca gcgtcagaat ctgccgacgt gtcagtccca gatgcgccag
     2641 cagaccaaat tccttgcggc gtgacagcac ctgtgcgctg aaactcgccg ccacaccaaa
     2701 caagccaatg ccaatcgcca ccgcctgcag ccaataggtg acggcaaaac tgcggtcaaa
     2761 gaccttgagc gagatggccc gaatctgcgc ggcggtgcca aattcgagca agccaccggt
     2821 gccatcgcca cgggtcgggg tggctgcggc ggggtcagcc agcaccagca gggcttgggc
     2881 aatgcgcgtg gcatcggccg ggtggttgag ccacagcgcc aggtcattgg cacgcaagtc
     2941 gccggtcagg tcgatgaagg cgcgccggtc aatcgcgatg gcaccgctct ggcgggcata
     3001 gtcacgccac acgccagcta caaaaaacgt agctggtggc gctttgttta taagggccag
     3061 aggtctaaaa gccttggaaa aaagcggaaa ggactggcct ggcctggcgc catgcagatc
     3121 aaccaccgcc tcgctgacgt aaattccggt ctgccccgct ggcaccggca gcggtgtgtc
     3181 gatcaatggc aggctgcgcg ccgggtcgcc tagctcgcgt gcgatcaggg ccaccgcagg
     3241 caggctgctt tgcagcatga cgggttgctg gcgcattgcc tgcagtttgt tgaccccggg
     3301 aacttgcgct gcggcggcga caaattcagg gctgaagaag accgtatcgc tggccccgag
     3361 gttgccggcg ctgcgcacat acaggtcggc tggcagcacc cggtcgagcc actgcgtgac
     3421 cgattcgcga aaactcgcca ccatcaccgt cagtgcaact gccaggctca gcgatgccac
     3481 gatgccgctg acggctaccg ccgccgtgtc acgctggcgg cgcgagcgct ccaccgccag
     3541 cagcggcagg gtttggcgtg ccagcagcgg ggccaaatac ccgagcagca ggccaatcag
     3601 ccagggcagg gcagtgatgc caccgagcag cagcaagccg accgacagat aagccgccaa
     3661 aggtatgcca aaaacggctg gagcgcttgt cagcattgcg ccaacagcta tcaaaacaag
     3721 actgacccag tgtgatttgg agcggctggt ggtgctgccc aggcccttga gcgtttgcgc
     3781 tggtggcagt tgcccggcgg tgcgggctgg ccaccagcca ccggccagcg cagcggccac
     3841 gcccaaactg ccgtaaaccg cggcagccgc agcgctgaac tgcaaggtcg gcgctgcccc
     3901 gccaaaaaag ccgccaccca gatcgccccc cagcaaccgc agtgccagtg ccgctagtcc
     3961 cgagcccagc gccagcccgg cggcgctgcc aaccagcccc agcgccagcg attcccacat
     4021 caccagtgcc agccgggccc gcccagacag ccccagcacg cccagcaggg caaactgctg
     4081 cgcccgcttg gcgacactca acgacaacac tgaaaacacc agaaacgccc cggtaaacag
     4141 ggcaatcagc gccagcactg tcaggttgac ccggtaagcg cgcgacaggt tgctgatctg
     4201 cgaccgggca tcttccgctg cagccacggt cacatcgcct ggccagtcag gccgggtttg
     4261 cagcgctttg gcaaacgcat cgcgatctgt gcccggctgc agccgcaggt cgatgcggct
     4321 gagctgcccc tgtttgccaa acagctcttg cgccgcgcca atgtccatca ccgccaatgc
     4381 gctgccgcca gcgcgcacgc tgcctgccac gttgaccgtc agccagccca tgccgctttg
     4441 caactgcagg ccaggtgctg caaacggcaa gtcagacgcg ttggctggca agctgccaga
     4501 tgctttgcca gcaggtgtgg ctggcatggt ttgctgcgtt gtgaaagctg ggttgggtga
     4561 aaccgccggt cttgccgggg ccgctttgac tggcacactg gccgggctgg ggatcacagt
     4621 gccgggcggg ctgttgacca gcagtcgccg tgccacggcg ttcaagaata cctgccctgg
     4681 cgcaaacagc acccagcgcc cggcacgcgc tggaccggtg ggttggttgc cgggcacctg
     4741 gtcggttgcc tcaagcgtgg cttggccagg ctcgggcaag ggcatcaaat ccggcgacac
     4801 gccgagcacc gtcagcgcgt cgataccgat cacccgcaag ctgcgcttgc cctgcgcggt
     4861 ggccatgtag ctgccgactt ccagcaccgg agaggccagc atcacgccgg gcatttgtgc
     4921 cacccgctgg tacagcgctt catcaaaatg cccctgcacc gcccgcagtt ccaggtcact
     4981 ctgacccgcc accgagcgcg tcgcctgggc aaactcatcg agcgccgaag cgttgatcaa
     5041 atgcactgaa aaggccagtg ccacccccag catgacggcc agcactgccg ccgcattgcg
     5101 ccacgggtgc tgacgcaact cctgccagga aaaggtcttc agcagttcga gcatggtttg
     5161 ttctggtcgc ttttacgcgc cttggtggcg gcttgcaacg tccaccagag gcgcctgggt
     5221 caggcacggg cgaaacgcaa ggcaaggcga gatgacaacg acatgggtga attggatatg
     5281 gaattggggc ctgcaatgtt gaacagatgc gcccgggcct ggcacgcgcg gtttgagtcg
     5341 gtaagggacc tggtagtggg cctgacccgg gcgcttgaca gaaatcgcgt cttttccgtc
     5401 ctggcgttgt tgaccttcct ggcacaagct gcgactgtgt cgtctgccct ggcctagcca
     5461 gaatggaaaa cccatcggct tcattttgta accattgtgt tgaaacaaac cggcaggttg
     5521 atgcaaacag ggcagagcct ggcgatgctc aggtttctga cggcgacaca ccgttgccgc
     5581 tatcccattg cacccggttg cgaccactgc gtttggcatc gtagaggcag tggtcagcac
     5641 agccaaacag ttctgtatca acctcattgt gcctgggcag ccaactggca ccacccaggc
     5701 tgaccgtcac ccagggactg gtggccgatt ctggaaacgc aatggccagc gcttgaactg
     5761 cagcccgaat cgcttcagcg acctgttggg cgaacccggc atcaatttcc ggcaacacca
     5821 ccacgaattc ttcgccgcca tagcgggcga caaaatcggc aggccgcttc agtgctgact
     5881 ggatcgcctt ggccacgcgc ttgagcacca ggtcacccgc tgcgtgacca taggtgtcgt
     5941 tgaaaatctt gaaatggtcc acatcgacaa tgatcagcga cagcggcctg ctgttgcgca
     6001 ggcagcgacg ccattcaagt ttatagacct cggtataacg ccgtcggttg ggtatttcag
     6061 tcaggctgtc gatcatcgcc agttgttcca gcaagcggcg ctggtgtacc gactgcaagt
     6121 ggttgcgcac ccgcacccgc acgatgggcg ggtgaaaagg cttggtgatg tagtcaacgg
     6181 cacctagctc caggccacgc tcttcgtcca gtgtggagtc cagcgctgac acaaaaatca
     6241 ccggaatatg ccgcgtgccg tccccggcct tgagcgcccg gatcaccgcc agcccatcca
     6301 tttcgggcat cagcacatcg agcaaaatca ggtctggtga gcgctcgcgc gctttttcga
     6361 gcgcctgtac gccgtttttg gccagaatga tctggtagtc atcttgcagc agttcggtca
     6421 gcagcgttcg attgtgcttt tcatcgtcaa caatcaataa tgtaaaactt gggttcatgg
     6481 tcgtaaagtt gacaaagttg cctgcaagcg cgccagcacg atcagtgcct cctggtattc
     6541 gacatcgtcg atcaggtcac gcagcaaaat gacatcattt tgctgacgcg ggtcgttcag
     6601 gcagattttg agctcgtcgc aaaggcgccc ggccgctgca tcgtcgcttc gcagcaaagt
     6661 tttcaaacgt tcgatcagac ccagttgtac atcaaatgaa gcggcgctcg ccggaccagc
     6721 cccgttgtca gaaagatgct ggacatcggg cagcgctgac agactttgca gcacccgccg
     6781 cagggccagc acaaacaccg gaaattctgc atcagcgcgc agcttctgtt catagcgttg
     6841 ctccagcgct ttggcgcatt gcgacagttc caccgctccg atggtggctg cggctgattt
     6901 catcgagtgc gccaggcgca gtgccacgcc aaagtcgcct tgcgccaggg cgctgtcgat
     6961 gtcgtcggca gtgctgccaa attcgcgctg aaagccagcc agcatctgcc ggtacagggc
     7021 gggccggtgc atgtggttga tcaggccacg cccggtgtcg atgccgtcga gtggtggcag
     7081 cgctggttca tatccgccat ctggtacccc ggcgccagtc ggtgtggcac cgtcggggcc
     7141 cgctttgcgc ggcgtgatcc agcgcagcag cgcggtaaac agcgcgtctg agtcgattgg
     7201 cttggtcagg tgatcgttca tgcccgctgc caggctgcgg tcccggtcgg tgctcatggc
     7261 atgcgctgtc atggccacaa tcggcaggca atcgtgcgcc gggttgctgc ggatggcgcg
     7321 ggtggcggcc aggccatcga gtaccggcat ctgaatgtcc atcaacacca ggtcgtagtc
     7381 gttctggttg acttttgcca ccgcctcttc gccattgacg gcaatctcgg ccttgatgcc
     7441 ggcttgctga aggaatgcca gcgccaccga acggttgatt tcgacatcgt ccaccagcag
     7501 cacacgggcc tgccggatgc cgctgaaatc gtactggcgc tcgcgctcga ggtctcggtt
     7561 ttgctggtgc gcccgggcca gcggggctcc catcaagact tccagcatgg catcgtgcag
     7621 caaagattcg ctgaccggct tggacaatac ccggttcaac ccgactgccg cgagcttgtc
     7681 catgatcttg tcataactgc cagccgtgat catcaacagc gctggcgcac cgcccagtgc
     7741 cgccgcgttg tcctgcatgc gctgggcggt ttgcaggccg tccaggtcgg gcatcagcca
     7801 gtccagcaac accagtgcga acgggtcacc ggcggttgcc gcagcgctga gcatgtccag
     7861 cccctgttgg ccgccagacg cggcctcggc ccgcatgcca tagtgctgca gcatctggca
     7921 cagcgccagg cgggcgttgt ggttgtcgtc gatgaccagt acatgcttgc ccgccagatg
     7981 atgggaatgc accgctgtcg gcaactggtt gtcagtcacc gtgaagcagg ctgtgcaatg
     8041 aaagcggctt ccccggccga ccatgctttc aacgccaatg tgcccaccca tcagctcggt
     8101 gagctgcttg cagatggcca gccccagacc gctgccacca aaacgcctgc tgatgctgcc
     8161 atcaacctgg gtaaagggac aaaacagatc agccagccgt tcgtgcggaa tgcctatccc
     8221 ggtatcgctg acggaaaacg ccagctcgac ctgttcgcca ggccgagcgg gcgctgaacg
     8281 ttcatcggca cgtttgaccc gcaccacgac atcgcctgcc tgcgtgaact tgacggcgtt
     8341 gttgaccaga ttggtcagca cctgacccag ccgcatcggg tcaccgagca ggtggcgggg
     8401 tacggcttca tcaatcagca tcagcagctc cagccccttt tcctgtgctt tcacggccat
     8461 caggttcgac aggttgtgca tcacctggtt caaattgaac gggattgact ccagcgtgag
     8521 tttgccggcc tcgatgcggg agtagtccag cacgtcgttg atgaccccca gcagggcatc
     8581 ggacgaggtc agcacttttt cctggtagtc gcgctgctgg gcatccagcc gtgtcttcag
     8641 cagcagccgc cccaggccga tgatggcgtt catcggggtg cggatctcat ggctcatgcg
     8701 tgccagaaaa tcagatttgg cctggttggc ctgattggct tcgtcggttg ccagggtgag
     8761 ctggtgggtg cgttgcgcca ccagttgttc caggttttgc tgggattcct gcagttctag
     8821 gtcgcgcccc tgcagcgtgt cgatcatggc gttgaagccc ttgcccagca gcgccacttc
     8881 gtcgtcgccc tgccgatgaa agcgcgtcgg cagggtggca tcggttgaca cagcggtcat
     8941 caactggctg agttcctcca agggggatgc cagacggcgc accagacgga ccgacagcca
     9001 gagcgacatc ccgagcgcgg ccagcaggat gagcccgctc tcaagtgccg tttgcagcag
     9061 cgctgctttc atttctttga gcgaggcgct cagttcaacc acggccgggc tgctggcatc
     9121 acccggtacg gcggcccaga ccgtcatcac gtcgtcggca acctgtgagt tggccagggc
     9181 cgggggcatc accaacagca gcgggtctgg ctggctgctt tgatattgcg caaaaacctg
     9241 gccattttgg tccatcagcc gggcccgcaa aatcgacggg ttgctcttga aggcagcaag
     9301 cagccgtgtc gcctcgacac ggtcttcaaa agccaccgca gcctggctgc taatggccac
     9361 caggcgcgcc tgcgtcgcca gtgatgatgc ccagtcgttg tgaatcagcc gttgctgctg
     9421 cagcaccaac agcgcaaaca ccagcaacac agccagcagc agcgacgcgc aggacagcag
     9481 caccagtttg ccgcgaacgc tgtgcgggtg cagccactgc gccagggtgc gctggctcat
     9541 ggctccacca ccctggccag ccgcaataac tgggcattga aggcgatgtc ttgcaggcgt
     9601 gcagccttgc gattgatcag gatgttgact ggcgcgccgc caccagccgg ggtcagttca
     9661 cccatgccac cggccctggc aaacccggca atgtccgaca ccgtcaccat tggctgggtg
     9721 gtcaggtgtc gcagcacggc agcaagcccg ctggccccgc tctgtgagac aaagacaagg
     9781 ccgcagcggc tggtttgtgc tgcagcaccg agcttgctca atgtcacccc ccgcccgtca
     9841 atcggcacca gcgtctcaaa ggcggcgttg aaaacattgc tgcccggcag gcaaaacgaa
     9901 aaaggccggt caggcgcccc gttggggcga tagacaaact gcggcaattt gaaaaacagc
     9961 gccgatttca gcgccgattt cagcgccgac ttcagcgccg attcagatac cgccacgccc
    10021 cgcgactgcg ctgccccctg ggttgccatc atcagcaagg cgatctggcc aggcgcctgt
    10081 cgtaacgccg ggtctcctgg accgaccgcg ctaacccggc aagctgtgct tggccatagc
    10141 cacagcatca gagcccgaag ccgagtcatg acaaggcctt caaggttgac acgcaagcgg
    10201 ggccatcgag cttctgggct gcgggcacca ccgtgccacc gagcgtggtg gcgctcttgc
    10261 gctggaaatc agcagggcaa agctgatcaa gcggggcttg atcggatggc tgccaatggc
    10321 caccgacgct ctgggccagg ccattttcac ttttcatgcc ggtcatgcag gcagcctcct
    10381 tgcgacacgc gacacgcgac acgcgacacg cgccaccgcc gggtgacgcc cggtttgcgc
    10441 tggcaccgtc cggcgggctg gtgatgatgt tgacccccag gccagcgcat cggcaccgca
    10501 aactgccgcc gccaagccga aaagtaactc aacctgtttg gcaggatgca gtgcaatgtt
    10561 ctccgccaca ggaaggctgc cgcctgctgg gttgccaatg tgcgcgccgt ccatcagcag
    10621 cttgttcggc ccaaccaagc cttgcacggt gaaatgcctg caggccgacg acctggtgcc
    10681 gcgcttgaaa tcgaaatcgg gcaagtcctg gcgtaaatcg gtcgggttgc ggtagcgatg
    10741 ttcgcgaacc tgcgcgcggg tgaagaccag aatgcacgac gacattcggt caagcgtctc
    10801 cagttggcgc gacgcggtga cgaccgggat attgatcaga tccatcaatc acaagggcat
    10861 cagccggtca agcccggact aaaccgggtc agtggcgttg accactggta aaccggccag
    10921 accagccaga aaaatcaggg aaatgtggtg ttcgccaatt cgcatcgcaa tgccctctct
    10981 gtcggccagg gcattcaagc gacgaatcca aggcttggtt cgattttatc agatgagtgg
    11041 ttccgcacct gacccggttg caccaacatg atgcacccat tcagtttccc tttggctacc
    11101 tgggttgaac cagccgtgca tcaagccagc cgcgcaggct gttgatgaag gccagcgcct
    11161 gcaccctggg caggtcgccc agggtgatca ccgcctcttg cacaacgcct tcactcaggc
    11221 agcgtgcgcg gccgacaccg gccaacagcc cgcagtgcag cgccggtgtc agccactggc
    11281 cgtcaagcag cagggcgacg ttgccacgtg tgcactcggt caattcgttt tgcgggttgt
    11341 agagcaaggt gtcaaagatg ccggctccgg tcggggcaaa ggcctcgtag tgcgcccgat
    11401 gggtcgtttt aaagcgtgtg aattcgctct gtgatgcttc aaaaggtata gctgccagcg
    11461 caatggatac gggctctggg gtgcttttat gtataaaaat acgaacggtt ggctggccct
    11521 tgggatctaa ttgcaggcgc acccgccaga cctttggctg atgctgcgcc agcgccttag
    11581 cccgctgacc catggcagca gtcaagcgcc gggtgtggcc atgccggtcg ccttgcacag
    11641 cctcgctgct gcttgcgacc ctggtcaggg tctggcggat gcgcagttcg tcaaagggat
    11701 agccaaaatg cgcagcagcc ccttgcaggc gtgccaggtg tgcttccaaa ttgtgaaact
    11761 tgcggccttc gagccgcagc gtttccagca ggccgaaggc ggcgctggca cgctccagaa
    11821 agccgcgttt gtgctgccac tcgcgccatt cgtcatcggc agtggcatca aaggtgatgc
    11881 cactgccgat gccgcaggtg gccgtttcac cgtgcaagca aacggtgcgg atagccacgc
    11941 tgaaggtggc gtggccacct ggccgcacga caccgatggc cccgcaatag atgccgcgcg
    12001 gctgcggctc cagcgcacgg atcatctgca tcgcccgcac cttgggcgcg ccggtgaccg
    12061 agccgcaggg gaaaagtgcc gtgaacacgt ctgccagcgt ggtgccggga cgcgtggcag
    12121 ccgtcacgtc tgacaccatc tggtgcacgg ttggcaggct ttgcacacga aacagcgcag
    12181 gcaccttgac cgaatggggc agggcgatgc gtgacaggtc gttgcgcagc aagtcaacaa
    12241 tcatcacgtt ttcagcgcgc tccttgggcg acgtggtcag gcctgcggcc agcgcagcat
    12301 cggcagatgc agtggcacca cgcggtgccg tgcccttcat cggccgcgtc agtatctggc
    12361 catcctgcca gtcaaaaaac agctccggcg agaccgacaa gacctgctca gaaccggtgt
    12421 cgatcagggc cgcgtagccg ccgggctggg cgcgttgcaa gcgctcgaac aggccagcgg
    12481 cactgtcggc aaacctggcc ttcagcggtg cggtgtagtt gacctggtag cactcaccgg
    12541 ccgcaatagc ctgggcaatc gaagccatct gcgcgtcaaa ttcggggcga ctcagggcgc
    12601 attgccagtc aatcggtgtt tcatcagccg tctttggtgc ctgatcagca ggatcagcag
    12661 gatcaacgca ctctgactca ggccagggca gggcttgctc gaatacgcca aaccaggcca
    12721 gcggcgcatc aacgttgtgg gtgacatagg cgctgtcaaa agcgcctgcg gcctcataac
    12781 gcaggtagcc gacacaccac cggccctgtt tggccagggc ttgcacggca tcaagcagcg
    12841 gcttgacctg gtcggcagtg tcggcaatca aggtctgcag gggcgagtca aaagcgcagc
    12901 gcagcgctcc tgcgccaggg ttggtgccag cggcgctggc ctggtcggtc ggcgcggcaa
    12961 tgctggcgcg ggggtcgcaa aaatcaagcc tgatctgcat gcccggtcaa accttcggcg
    13021 gtcagcagca gcacccggtc ggcgcgggcg gcggcagcct ttgaatgcgt caccagcacc
    13081 attgccgcgc catgggcacg ggtctggctg accagtgcgt ccatcacccg ctcggccgtg
    13141 cgcgggtcga gattgccggt cggctcatcg gccagcacca gcatcgggcg atgtaccagc
    13201 gccctggcaa tggccacccg ctgcagttgc ccgccactga gttgctgcgg caggcgctcg
    13261 cccagatcct gcaaacccac tgcctccagc atcgccagca ccctggcgtt gttgtgctga
    13321 ttgagcagca tcagcggcag cgccacgttt tgcgccacgc tcaggtgcgg cagcacatga
    13381 aaagcctgga acacaaagcc ggtcttttcg cgccgcaagc gcgccagttg gtcatcgctt
    13441 tggcggctca ggtcctggcc atcgagcagc accgagccgt ggttcaaggt gtcaaggccc
    13501 gccatgcagt tgagcagggt tgacttgccg acaccagatt cgcccatgat ggcgacaaat
    13561 tcacccgcag ccacgcgcag cgagacgtgc ttgaacaccg gcacctcgcc gtaacgcttg
    13621 cccaagtggc tgatcaccag gctcatgccg ggttacagat gaaggcacgg gcaagcgtca
    13681 gtacctgtgc cacggcatca ggcgcgtcac aggccaccac ctggcgctgg cggttggcac
    13741 gcagccaggt gatctgacgc ttggcgagct ggcgggtggc aaaaatgccc ttgtcgcgca
    13801 gatctttcag gggccacagg ccgtcaagcg cctcccaggc ctggcgatag ccgacgcagc
    13861 gcatggcagg caagttgggg tgcaggtcgc cgcgtgatcg cagggccagc atctcgtcaa
    13921 tgaaaccatc gcgcagcatg gcatcaaaac gctgggcgat gcgctggtgc agccaggcgc
    13981 ggtcgcttgg ttcaagcgag atcagcaggc tgggatgcct catattttca tgatcatttt
    14041 ggcctgtagc acccgtgctt attgcgctac aagctacgtt tttaatagta tgaaaacttg
    14101 ccatcggcag accggtgatg cggtacacct ccagcgcacg ggcaatccgc tgggcgtcag
    14161 ccggggctag ccgggctgcc gttaccgggt ccaccagcgc cagttcggcg tgcagtgcgg
    14221 gccagccttt ttgggcggct tcttgttcca aggcctggcg aatggcgggg tctgcgcgcg
    14281 gcaggtcgtc caggccgtcc tgcagcgcct tgaagtacag catggtgccg ccaaccagca
    14341 gcggcagctt gccgcgctcc gtgatgtcgt gcatcagtgc gctggcatca gcgacaaact
    14401 cagcggcgct gtaggcatgc agcgggtcgc ggatattgat caggtggtgt ggcacagccg
    14461 ccagttcggc tgcatcaggt ttggctgtgc caatgtccat gccacggtag accagtgccg
    14521 agtcaacgct gatgatctca acccgatgct gcgcagcaat ggccagcgcc gccgccgtct
    14581 tgccggctgc agtgggaccg gccagcgcca ggtagcgtgg caggcgatgc gctctggcaa
    14641 ggggcgttgg gcaaaccggc cgggccgtcg gctggggtgg tggggtgggc tggggcatga
    14701 aatggatgcg ggtcggggca ctgatgttac gcaaattggc gaagaccttg tgtttcgggc
    14761 gcttgccggt tgatggcgtc ggttgcgtgg ccaaaaaaaa tgcagcttgc gcggcgcgcg
    14821 cgggccggat cggaaagagt ttgcccggca aaggccccgc cgcgctgcca cgacggcgcg
    14881 gacatccgct catctgggcg gtcgcagatc aggcagtccg attgctggcg aacccgatga
    14941 gccgacctgt cggtgttcag ccgcgcaacg cctccagcgc gttggccaga ttggcttcaa
    15001 ccggcaacgg ttccgccccc atccgggccg ccagcaattc ggcgcacaat accgaaaaac
    15061 tcaaacccct tgaacccaag ccggcacaga tccacagacc gggttgcctg ccagcatcaa
    15121 gcggccccac cagcggcatt cggtcactgc tgacacagcg ggtgcttttc caggtgttca
    15181 gcgcattcga tgcaaacatt ggtgccaact cgctggccag agcgggcaag agtttctgca
    15241 agtgcgtcag attgctcgcg tggttatcct gatcagcacg ctcggctccg ctgtctggct
    15301 ggtacgacga cccggcaaac caggcttggc catcctgagt cggaacctgc gaaatcatgc
    15361 tgcccgagcc gttgaccggg caaggcgcaa agtgaggtgt tgcgcttgct gaatctgcgt
    15421 gccgggccca gctcagcagc ccgcgcatgc cctgggtggc tggcagatcc ttgagtcgtt
    15481 gcgccagttc gaggctttgc gacgccagcg cctgcagcag atcccgcgca ccgcaggcgt
    15541 tggccagcac gactcgctct gccacgcaca aaacggcccc ggttgcatcg agcaattgcc
    15601 agacacctgc gtcaaaccgc aggctgctga ccctggcgtt ggtttgaaac gacacaccgg
    15661 gctggcgcag ccaggcgcgc accagttcgg ccggtttgat ccaggccgct ttggcgtgcc
    15721 aaaggctggg ccccaaagtt gcagtggtgg caccggcagg catcgcgctg gcctgcatgc
    15781 accaggcttt accagcgtcg ggccattggg cgggcaactc aggcgtgcca tcgatttgtc
    15841 gctccagcac gccgctgggt gcccactgct gaaaggcttg caggtgttgg tcggcttgtt
    15901 gcaacatcat ccgcacaccg gcacgtgaca gtcgcgacag gctgcagtcg tcgctggaga
    15961 catgtggcac caccaggcca accggcaaac ctgatgcgcc cgcggcggca tctgcgccct
    16021 gatctagcac caacacctgc cagccgcgcc gcgccagcga cgccgccacg ctggcaccgg
    16081 ccaggccagc gccgatcacc gcgcagcgtt gaaccggcag tgcctgcgcg atgccgggtt
    16141 gtcgcgcgct tttgagtgtc caggacgggt gaaaaaccgc ggtcaactgg aatgcggcaa
    16201 tgcccgcctg gtgcgcaggc ccgcttgcca ggtcgaatcc gcattgcttc aggtggctgc
    16261 tcaacgcggc caaatcagtt ggctgcgcgc caaggcactg cagcctggtg ccgcgccggc
    16321 aacagcgggc cagtgccttg attgaccaca gggattgggc ttcaagcgcg ccgggcggct
    16381 tatcggcagc aagtcgcagc gtcacccaat cggcatgaaa ctgctgctgg cgcagcaggt
    16441 gcatcgcatc acccgcgcac agcgttagcg tgacttggcc atcggcgagc aaaaaacgat
    16501 gaaagccggg aagaattcca aaccattgct ttgccaggct tgctgccagc gcttgcagat
    16561 caggctcatc ggcgcagcgg tctgtcagcg attgtggcga aggcgcaagc gggcacaggg
    16621 ccacatagtg caatagccgg gggcgatccg ggtcgcgttg ccaggccagc caggtttcaa
    16681 agaaggcccg tccatggtgg aaatcagtgt ccagcacccg ccaactggtg ctaccttgcc
    16741 agagttttgc ggctgcctga tcggcctgca caacgccagc ttgcggtgca ggcaaacctt
    16801 tagcccttgg gtggcacgta gccctgggcg gcttctgcac cgccaccaaa gaaatggttt
    16861 tccatttgcc ttgccaggta ttgccgcgcc cgcgcatcag ccaggttgag ccggttttca
    16921 ttgaccagca tggtctggtg cttgagccat tgaccccagg cttccttgct gacgctttgc
    16981 cagatgcgtt tgccaagttc accggggtaa ggcggaaagt ccagcccttc ggcctcaact
    17041 cccagtttga tgcaattcac agtgcgcgcc atggttaccc ttttcgtgta tcggtcaaaa
    17101 cgtcaacttt agcaagcttt tggacaaacg ccactactgc ggtaaaatgc gaccctatgg
    17161 caaaagcaga taccaaaacc aaaattgtgg ccgatggcct gcgttacaaa tccaaggtgt
    17221 cgggttcgcc cttcaccgcg gcgacttcgt ttggcgcggc cacgatgtcg tgctttctgt
    17281 gcggcaagca ccgcgcgcgc tctcaaatgg tgtccaagaa gattttgggc aagtctcagg
    17341 ctgtttgttc gccttcatgc aaggcgcttg acgaagccaa taagtagcga atgttgtcca
    17401 tcttgtgctc gttgccagac ggcgcactca gacgcgtcta tctggcgttg agcgccgttt
    17461 gttttggcat gctggcgttt ggcctgtatc tgcaacacag cgttggcctg gacccgtgcc
    17521 cgatgtgcat tgtgcagcga tacgctttgt tatttgtagc tatcatcgca ctgctggcgg
    17581 gcgctagcgg caagaaaatg cttcatttat tgggtgccgc gctgcttctg ctgggggccg
    17641 ggtttggtgc ctttgtcgct gcgcgccaga cctggctgca gtggtatcca cccgaaatcg
    17701 ccacttgtgg ccgagacttc tacggcatga tcgaaagttt tccgctcaac cgcgccattc
    17761 cgatgatttt ccgtggcagt ggcgattgct ccaaggtgga ttggacgttt ctgggcggct
    17821 cgctggccaa ctggtcgttt gtcagcttta ccttgatcgg cgtgctggcc ttgctgattc
    17881 tttggcgcaa cgccacgcag cgtgtacgct gagctgagct atagcagggc actgctgggg
    17941 tgttcgcccc gctcatagac cacatgcgcc cgtcccacca gcaaggtatc aagcgatcca
    18001 atgttggacg atgtcttgtt ggcatagggc aatttgtgca agacaaagcg cattgcgttg
    18061 agccgggcgc gctttttgca gtctgacttg atcaccgtcc agggtgcatc agccgtgtcg
    18121 gtctcgaaaa acatggcttc cttggccttg gtgtagtcgt cccatttgtc cagcgacgcg
    18181 aggtcaatcg ggctgagctt ccattgtttg agcggatgca gttcgcgttc cttgaagcga
    18241 cggcgctgct ccttgcgact caccgaaaac cagaacttga tcaggtaaac accgctttgc
    18301 accaactggc gctcaaacag cggtgcctga tgaatgaact gctggtactc ggcctcgctg
    18361 caaaagccca tgacttgctc cacgccggcc cggttgtacc aggagcggtc aaacagcacg
    18421 atttcgccag cggtcggcaa atgctggaca tatcgctgga aataccattg gccgcgctcc
    18481 gattcggtgg gtttctcaag tgccaccacg cgcgcgccgc gcgggttcag gtgttccata
    18541 aaacgccgga tggtgccacc tttgccggcc gcgtcacgcc cttcaaagag aatcacgacg
    18601 cgctggccgg tttctttggc ccagctttgc aacttgagca gttctacctg caatttgtat
    18661 ttgtcagact cgtagcgctt gcgcgacaac aggttcttgt aaggataacc gccgctgcgc
    18721 cagtcaagcg acagttcttc gtcaggcgcc attgccttcc cgtgcgggtc gagctgaccg
    18781 cccagcagca tcagacgaat cgccgcgatg tcatctgatg aactgccagc cagcagggtt
    18841 ttcagtgcat cggccttgtc aggcgcgttc gccaaaatgt ccctgatggc ggtttgctgg
    18901 ctaccttgcg cggcatccag gctggcttgt acggcgtgcc tggcaacgcc tgcggtggtt
    18961 tttgcaccac tggtgtcgcc cagaaccgtg cggcgcacaa cgctcgcaag cggttttctg
    19021 actgccactt ttctgcttgc ttttggcggt gccgccgctg tcgtcacagt tgtctcggac
    19081 acttcgcttg tctgggtttg atttattttc ggctctgcca tcgaaatctc ttcaggttgg
    19141 gttgtttttc atgccaggcc gtacagtgcc ggaaattccc gcaggtgtca aattctgacg
    19201 accaactcac gaattgtact ttgatttagc tatattttaa atagctgcca gcgcaatctt
    19261 aacaagggct agaggcacat ttctttcact ttttgcatcc ggcccgcctg ggggttaggg
    19321 ggcgggccag catacctgac gcatcacagt ggcagggcgt caaacgactc gtaagttctt
    19381 ttcagccagg acttgacacg ctggcgctcc agcaggccta gtgcatcgac atccgggcga
    19441 ctgttgacgg ccgcccagaa actcacgttt tgtcggtcaa tcttgcgcat gctgccctca
    19501 tgcagttgcg ccagcgtgat cgttctggcg ccgaaggccc gcgccttgac catggcggcg
    19561 gattcttcga gttgagaaaa gtctgagccg cgcccaaggt tcttgacgac caggtaattg
    19621 gggccttcgc cataggtgtc gagcaggtga tcaagcagtt caaccgaatc gttgccggca
    19681 tcggcgatgt gccagaaatt cactgctatg cccatgtcgg ccaacaaggc cagcaattcg
    19741 gagtcattga tccagcgtgc cagcggcgcg gcggtttgtg ctgcaaggtc cacgatgacg
    19801 cgaggtttga tcgccgcatc cgcctcacct tcaaaaacac tcaccacctg gtcgagtcct
    19861 tcgtagctgt ccactagcac cgggctggca tagtccgcgt aaaagcgggt gaacgaggtg
    19921 tgtgaacgat cggtgtcaaa gccgacaaag ggcaactgtt tgtcaataaa gtactgggcc
    19981 agcacgcgtg ccaccacaga cttgccgaca ccaccttttt cgccgccgat gaaattgagg
    20041 gaactcatga aattatcctg ttgtgttagt tgcaaaagcg taatgacctg acttgatctg
    20101 caagcaagtg tcgtgctcaa aaccggccaa cgcgccgcga atcagttgat ttttttgacc
    20161 agtacctggc tttttcggtc ccagttgtac ttgcgcttgc gcgcctcggg cagccaatcg
    20221 gggtcggctt gttcaaaacc acgtttgatg aaccagtgca tggtgcgcgt ggtcaagaca
    20281 aaaatgctgt cgagcccagc cagtttggcg cgctgctcaa tgcgtttcaa gacccgctcg
    20341 ccgtcgccct ggccctgcac atcgggtgat acggtcaatg cagccagttc cgccgtgcgc
    20401 gcatcggggt aggcgtacag tgcggcgcag gcaaaaatca cgccgtcgtg ttcgatcact
    20461 gtgtagaggc cgacatcgcg ttcgatttcg gtgcggttgc gcttgaccaa ggtgccatcc
    20521 ttctcgaacg gttcaatcaa tttcagaatg ccgccgacat cgtccacact ggcctcgcgc
    20581 aggctctcca gcttttcatc gaccaccatg gtgccaatgc cgtcatgcac atacacctcc
    20641 agcagaatcg agccatccac ggcaaacgga atgatgtggc tgcgctcgac accgcccttg
    20701 caggccttca cgcaatgctg caggtaacac gcggtgtcgc cgggcttgtc ggcaccgggt
    20761 aaatcagcca gcaatttttc ggctgcggcc agtggcagtt cggtgatgat cgggttttct
    20821 tcgctgacgg cctgcctgac atcagtggcg atgcccggca cttcggtgac aaaaatcagc
    20881 ttgtcggcct gcaacgccgt cgcaaccgag gtggcgacct cttccatcgt caggttgaag
    20941 gcttcgccag tcggtgaaaa accgaatggc gagatcagca ccagcgcgcc catgctgagt
    21001 gaattggtga tgccagcggt gtcaattttg cgcaccacgc ccgagtgcaa aaagtccacg
    21061 ccgtcgacaa taccgaccgg tcgagcggtc aagaaattgc cggaaatgac ccgcaccgtg
    21121 gcgcctgcca tcggcgtatt gggcaagccc tggctgaaag cggcctcaat ttcatagcgc
    21181 aactggccag cggcctcctg cgcgcagtcc agcgcgactt cgtcggtgac gcggatgccc
    21241 ttgaaatagc gtgcggcatg tccttttgcg gtgagttgtt cgttgacttg tggccgaaaa
    21301 ccatgcacca gcaccagcct gacccccatg ctctgaatca gcgccagatc ttgggccaga
    21361 ttgggcagct tgcccgctgc aatggcctcg ccacacacgc ccagcacaaa agtctggttg
    21421 cgaaacttgt gaatgtaggg cgccaccgag cggaaccagg gcacgaaagt gaaattgaag
    21481 ggaagactca tgggtgcatt tgtgcagcaa ttgataacga tgaaaagtgt agctgcagtg
    21541 cgacaccgct tgacttcgca atgactggct ggccaagctg gttgtcaact tctgccaccc
    21601 cttgctggca tttgcgcgcg cggctagcgc tgccaccccg gcagtggccg gggcgggccg
    21661 cgttccgcag cgcatctgcg ctcgggtgct ggtaggcgct gacgcaaacc tggcaagagt
    21721 caccttggcc gagcatgtgt tattgcattg gcaaatcgtc cgtgcccagg gaccttgccg
    21781 caggctttgc taacgcaagg catggcgaga taacaacgac agggatgatt tagaaatggc
    21841 atgagttttt gccggaaaat gatcgcatgg tgtgcaatcc agtttataaa gcagcatttt
    21901 caattgctgg caatcgattt tgcgatcacg ctagcaataa aaaaccctaa gtaaatcaat
    21961 cacttagggt tatatactgg cggagcaggc gggattcgaa cccgcggagg gttttacccc
    22021 tcgcacgctt tccaggcgtg tgacttaaac cgctcatcca ccgctccgtt gggcgtattt
    22081 taactgagcc agcaggtgga ttgaggtgcc acatcaaaag gcaaaagaaa aaggccacac
    22141 tggagtggcc tttcttgagt acaaaccaaa ccgaattagt tcagtgctat cttcttgcca
    22201 gccttgtatg ttgagagggt catggtcggg tttttcattt cgccagaggg ctcaaatgcg
    22261 atttctgcag taacgccctt gaatttggtc tcgatcagct tgggtgtata aacctttgga
    22321 tcggttgagt tggcgcgctt cattgcatca accagcacaa atgtcgcatc ataggtatag
    22381 gggctgtaga tctggaactg acccggatac ttgctatcgt atttggcttt ccaggccgtg
    22441 ccaccaggca tcttggcaat ggatgaacca ccctcagcgc agacaacacc gtcaagggtt
    22501 tttgcacccg ctgcgatctt gatcaactcg ttggtgcaaa tgccatcacc gccgaagtac
    22561 ttgatgttgg acatg
//
LOCUS       DAOSLL010000005        20639 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_2832_length_20639_cov_4.749660, whole genome shotgun sequence.
ACCESSION   DAOSLL010000005 DAOSLL010000000
VERSION     DAOSLL010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 20639)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20639)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20639
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2832_length_20639_cov_4.749660"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="PLB25_00415"
     CDS             complement(<1..750)
                     /locus_tag="PLB25_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOE40090.1"
                     /translation="MANAAPPDPLVQRYLDHARFEKRLADRTLLLYTLDLQKLKHSAA
                     LAGIGLLQVQSSQIRRWVAQMHAGGRTGRGIALILSGWRGFYAWLGREGLVACNPVQG
                     VRAPKAAKPLPKALGVDDAVQLVSFQSASADPWLEARDAAIAELLYGGGLRLAELTGL
                     DVRASNQARGWVDLQAGEAHVLGKGSKRRIVPVGAQAVLALQGWLALRDQMPKGHARQ
                     APALAAATPGSVTAGAAAEQAALFIGQRGTRL"
     gene            complement(897..1616)
                     /locus_tag="PLB25_00420"
     CDS             complement(897..1616)
                     /locus_tag="PLB25_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HOE40091.1"
                     /translation="MPNPSSLPSAIGSPITEEDIANYLAITPDFFARHAELLAAVQLS
                     SPHNQRTVSLQERQALLLRDKIRMLEQRIMDMIRNVNENMVLSDKLLLWARSLLLNTD
                     VLALPQLIADGIADQFSVPQVGIKVWGVAPAYVACPFALGVSDDAKSFATSLTEPFCG
                     VNTGLEAISWLPEPQAVVSLAILPLRSTPVGAALSGGIAPAFGLLVLASPDAQRFAST
                     MGTDFLARIADLTSAALSILR"
     gene            complement(1619..2494)
                     /gene="dapF"
                     /locus_tag="PLB25_00425"
     CDS             complement(1619..2494)
                     /gene="dapF"
                     /locus_tag="PLB25_00425"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HOE40092.1"
                     /translation="MQIPFTKMQGAGNDFVVLDETRTRYGLSAADYRFLGDRHFGVGA
                     DQILTVRASSAPGIDFSYLIHNADGREVEHCGNGARCFLAFVRRQGLTTKDVVRVQTM
                     NRILELQWQPDGRVTVDMQAPDFELAHLPFDATGLLPQASGLWQKWPLALDATARPAI
                     VEVALVSMGNPHAVLLTDDVERAPVATLGPQIQSLSAFPGGVNVGFMQVQSRSQIRLR
                     VYERGAGETLACGTGACAAVVAGIRLGLLAASVDVQTHGGQLTIAWQGGRAPVLLTGP
                     ATAVFDGQIELQPSL"
     gene            complement(2588..2959)
                     /locus_tag="PLB25_00430"
     CDS             complement(2588..2959)
                     /locus_tag="PLB25_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HOE40093.1"
                     /translation="MDIPALQASLRTFAAERNWQPFHTPKNLTSALMVEAAELLEIFQ
                     WMTADESKAAHLDPVTHERIADEVADVFLYLLQVADHCAIDMPGAVARKLVKNASKHP
                     ALQPGRAVASQPASQPTSQGS"
     gene            complement(2959..3498)
                     /locus_tag="PLB25_00435"
     CDS             complement(2959..3498)
                     /locus_tag="PLB25_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40094.1"
                     /translation="MSLRELLIMLYLSLDAHALGVLLAALGWGLGGTLLARIAKGGRS
                     QHDGRLIASVVIGGAVLLLVAALLAVGLAHVAFDQGLMDANILLLLAPLACVLLSVLG
                     IHQVFPLSELASVRTLGDVAGFALTCLALLWLLSKFRGWGVVFLGGLGSLALFGLLAA
                     LLLRVLYRRAFGRSEPGRN"
     gene            3676..5373
                     /locus_tag="PLB25_00440"
     CDS             3676..5373
                     /locus_tag="PLB25_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE40095.1"
                     /translation="MPRPSQLLHPPRPPATLRPAATVLLLRDGMDGFEVLMTRRSAAA
                     SFLPGAFVFPGGAIDAADANCHAIARRRPSQSDLHLTQAIAAIRESFEELGVLLAHRA
                     DGSAVDQGDIARLDRSASFAGQCAEQRLTLAAHEVFVLAHWMGDRDLARRFDVPFLVA
                     RMPAGQTPVADGSEQFEPVWVRPADALARHKAGDFFIIFPTIRTLERLQHFADVAAVL
                     QACAVTEEPLWTCCPRSGLLGGREARFMEHEAPFGELALVCPDGQIVHPLDWQSERAV
                     PLLKNVQRLTAPNPGAMTGPGTNSYLVGDPATGYIAIDPGPADAEHLDKLWRAAGGDI
                     RMIVCTHSHPDHAPGARPLQAMCQTAPPVLGLASAATARAASAFAPDRMLLNRELLTL
                     SPHGLEGDLAYETGHTLQVVHTPGHAANHLCLVLLQDGLLFSGDHILNGSTTVIDPPD
                     GDMNDYLGSLDVLSAACAQLRIEYILPAHGYVIGGPTDEAFSAIARLKKHRLQREAKV
                     MQAMQAQPHGSLDDWLAVVYEDVPERMWPVARRSLQAHVTRIESMILDSSADRLFHL"
     gene            5784..6605
                     /locus_tag="PLB25_00445"
     CDS             5784..6605
                     /locus_tag="PLB25_00445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HOE40096.1"
                     /translation="MPAFIITCLGLLLVLWFVGQPWWLERQRQTRRQAPFPSAWRDVL
                     RQRVALYRILPSHLQLQLKQHIQVFLAEKTFIGCNGLEITDEMRVTVAAHACLLILNR
                     PGDYYPKLRQILLYPDSFVVERDHTDGIGVAHRARQVLAGESWSQGQVILSWQDTLAG
                     AAVADDGQNVAIHEFAHQLDQETGSANGAPLLARRGQMQSWSNVLGAEFATLQARVTR
                     GAPSLFSDYGATDPAEFFAVVSEVFFEQPSPMAQDHPALYQQLSEYYLLDPLNWS"
     gene            complement(7231..7446)
                     /locus_tag="PLB25_00450"
     CDS             complement(7231..7446)
                     /locus_tag="PLB25_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40097.1"
                     /translation="MNNESTLAKAGSALARTASLIFGMPFDIARANYAKAVRFGLIPD
                     SLLRSALFERELAAAERQTLGLWARRV"
     gene            complement(7458..7640)
                     /locus_tag="PLB25_00455"
     CDS             complement(7458..7640)
                     /locus_tag="PLB25_00455"
                     /inference="COORDINATES: protein motif:HMM:NF023508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3563 family protein"
                     /protein_id="HOE40098.1"
                     /translation="MCLLTQLIEALSPGIRSQQDLDFAYLSQACDTVDLERRMREIDH
                     RDRHASPGRAFGLNTP"
     gene            7947..8486
                     /locus_tag="PLB25_00460"
     CDS             7947..8486
                     /locus_tag="PLB25_00460"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HOE40099.1"
                     /translation="MQEQNGRPDNGAARSPGALPTGLKWGPLGIVLFWLVLMGLLYAL
                     MTHYLKPQPVRITASGDLIIPKARNGHFYAAGTVNGRPVNFMVDTGASLVTVSEAFAQ
                     QAGVAAGVPTVFKTANGDLPGRVVPDVPVTLGPVQVSGVRLGVGLVGGDLNDGLLGQS
                     FLSKFELIVTKDQMILRPR"
     gene            complement(8501..9412)
                     /locus_tag="PLB25_00465"
     CDS             complement(8501..9412)
                     /locus_tag="PLB25_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009668116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOE40100.1"
                     /translation="MRPYTFKQIQTFLEVARQRSVSKAAERLFVTQPAVSMQLRQLEE
                     AFGLALVEPLGRNIRLTAAGQAFQAHATAAMGQFRDLEALMAEHVGLQRGRIELAVVS
                     TSKYFIPMLLVRFGRLLPGIDVQLNIDNRENVLGLLTRNEADLVVMGRAPSNLDCEAT
                     AFATNPLAMVGAPDHPLARRKNLPFSALADHGFVVREEGSGTRAAMQHLFAQNQTSMK
                     VVMAMPSNETIKQAVMAGMGLSFLSMRTIRHELASGHLALLDIQGLPQINHWYVTHLS
                     SKKLSPAARAFKAFLIEQGAALMDTWS"
     gene            9555..10283
                     /locus_tag="PLB25_00470"
     CDS             9555..10283
                     /locus_tag="PLB25_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOE40101.1"
                     /translation="MANAALKALIFDVDGTLADTESAHMAAFNQAFAEQGLDWVWTDD
                     MYLELLHVSGGKERILHYWQQRDPAILQADAPALQAQINRLHALKTVAYEQAVNRGAV
                     RLRPGVLNLIDEARQADLQLAIATTTSPANIAALLRHAMGADWRLNFSAIGDAGTAPI
                     KKPHPQVYLQVLGALKLPAAQCLALEDSRNGLQAAMAAGIATIITPNPFTAHHDFTGA
                     LRVVPDLSQIRLTDLCEWQNSARA"
     gene            10330..11028
                     /gene="rpe"
                     /locus_tag="PLB25_00475"
     CDS             10330..11028
                     /gene="rpe"
                     /locus_tag="PLB25_00475"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009523618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HOE40102.1"
                     /translation="MSSSPVLRLAPSILSADFARLGEEVRAIEAAGCDLVHFDVMDNH
                     YVPNLTIGPLVCEAIRPHVSIPIDVHLMVEPVDSIIPLFAKAGANIITFHPEASKHVD
                     RSLSMIRELGCKAGLVLNPASPVDWLDHVMDKLDMVLLMSVNPGFGGQKFIPSTLAKL
                     RLVRARIDAHVAAGGQPIWLEVDGGVKTDNIFEIVQAGADTCVAGSAVFGAKDADGGY
                     KTVMQALRRAAHPS"
     gene            11067..12164
                     /locus_tag="PLB25_00480"
     CDS             11067..12164
                     /locus_tag="PLB25_00480"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HOE40103.1"
                     /translation="MTLRSNRSTLTQFLIEERRRFPSASGDFNALVLDVALACKGIAK
                     AVAYGELAGGTGKDTADLGGALHLPGETQKKLNQLSNDVFIQRTEWSGNLAGIASEEM
                     DLPYQIPKQYPRGNYLIVFDPLDGSSNIDVNMSVGSIFSILRAPQEVIDSGRDVVDAD
                     FYQPGCKQMAAGYAVYGPTTMLVLTVGDGVNAFTLDPNLGEFMLTHPKLRIPEDTQEF
                     AINASNARFWQAPVKRYVDECLAGKTGVRGKDFSMRWIASMVAEAHRILMRGGVFLYP
                     RDAKDSARAGRLRLLYETNPVGMIMEQAGGRASTGQGPMLQVQPTELHQRIGFVFGSK
                     NEVERIERYHREPVLVELTNPLFAERSLFRD"
     gene            12257..13132
                     /locus_tag="PLB25_00485"
     CDS             12257..13132
                     /locus_tag="PLB25_00485"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013953005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HOE40104.1"
                     /translation="MSIRHPIIAITGSSGAGTTSVTRTFDNIFRRENVKAAIIEGDSF
                     HRHDRKSMKIAAAEAEAAGNHNFSHFGLENNLFSELETLFATYAKTGTGKRRKYLHDA
                     EEAAPYKQDPGTFTPWEDVPSGTDLLFYEGLHGAVVTPEINIAQHPDLLIGVVPVINL
                     EWIQKLYRDKKQRGYSTEAVTDTILRRMPDYVNYICPQFMHTHVNFQRVPMVDTSNPF
                     ISRDIPSADESMVVIRFANPKGIDFQYLRNMINDSYMSRANTIVVPGGKMELAMQLIF
                     TPFVWRMMERQKKAS"
     gene            13425..15506
                     /gene="tkt"
                     /locus_tag="PLB25_00490"
     CDS             13425..15506
                     /gene="tkt"
                     /locus_tag="PLB25_00490"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HOE40105.1"
                     /translation="MTTPTPELAKQMANAIRMLAVDAVEKAKSGHPGAPMGMAEIAEV
                     LWNRHLSHNPINPHWANRDRFVLSNGHGSMLIYALLHLTGYDLPMSELQNFRQLHSKT
                     AGHPEYGMTPGVETTTGPLGQGVANAVGMALAEKLLAVEFNREANGARHTIVDHFTYV
                     FLGDGCLMEGISHEACSLAGTLRLSKLVAFYDDNGISIDGHVEGWFTDDTPKRFEAYG
                     WNVIAAVDGHNPAAIDAAIKAARAQAVLPDGRPTLICCKTVIGKGSPNKAGSHDVHGA
                     ALGAAEVAAVRDELNWVSAPFEIPADIAAAWNAVARGVVAEHAWRVRFDAYSAVYPAQ
                     AAEFERRMAGNLLPDFADKLPGLLETIAANTTAFATRKASQNALDVIAPLVPEFFGGS
                     ADLTGSNLTNFKGCVRAGRDVWGNHLSYGVREFGMAAIMNGMALHGGFIPYGGTFLTF
                     SDYSRNAIRMAALMKIRVIHVFTHDSIGLGEDGPTHQSIEHIPSMRIIPGLDVWRPAD
                     GLETAVAWAFAVERRDGPSALCLSRQNLPRLTDMSMAVNIRKGGYILSDMQDAQAVIV
                     STGSELEIAMKAQALLAADGIATRVVSMPCTNVFDRQSNAYQEMVIPFNTPSVAIEAA
                     HPDFWRKYVGRHGVVIGMPTFGESAPAPKLYAHFGITAERVVSTVRTIVHRAANKRDD
                     ALPDTVVPSDN"
     gene            15521..16192
                     /locus_tag="PLB25_00495"
     CDS             15521..16192
                     /locus_tag="PLB25_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOE40106.1"
                     /translation="MKTFDLVMFDLDGTLVETAPEIMDAVNDTLGHFDLPLVSQQQVN
                     NWIGHGTLTLLVDAVAFATGRSAADVRASELLQSMVQEYNLFYQRRCGTRSHLYPQVR
                     ETLVALRAQGVRLAVVTNKESRYTQIVMDVQDLSALFDTVISGDSLPTKKPDPAGVAH
                     CLAQWGVSADRALFVGDSSIDVATARNAGVAVWAVPYGYNMGQPIEACTPDRIIADCS
                     ALLNS"
     gene            16255..17448
                     /locus_tag="PLB25_00500"
     CDS             16255..17448
                     /locus_tag="PLB25_00500"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOE40107.1"
                     /translation="MKVLRFADVIASGAAKGKRVFIRADLNVPQDDAGNVTEDTRIRA
                     SVPCIKMALDAGAAVMVTSHLGRPTEGTLTPEDSLAPVAKRMGELLGREVPVVANWVN
                     GVDVKPGQLVMLENCRVNKGEKKNNEELAKKMAALCDVFVHDAFGTAHRAEGTTYGIA
                     QFAPIACAGPLLAAEIDAINLALANPKRPLVAIVAGSKVSTKLSILQALAKNVDNLIV
                     GGGIANTFMLAAGLKIGKSLAEPSLLADAVAVIAAMKARGADVPIPTDVVTAKTFAID
                     AVATVKAATAVEDDDMILDIGPDTAAKLAKQLMEAGTIVWNGPVGVFEFPSFENGTKT
                     IAQAIAASSAFSIAGGGDTLAAIAKYGIEKDIGYISTGGGAFLEVLEGKTLPAFEILQ
                     KRAAG"
     gene            complement(18064..19389)
                     /locus_tag="PLB25_00505"
     CDS             complement(18064..19389)
                     /locus_tag="PLB25_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HOE40108.1"
                     /translation="MTMSLEKVLELLKKQKRVEGMVHSQNMPRQAIVETLVQRQHLAE
                     LQNLLAQWPASEIGSVLDALPIDDARLLWAQIPLARQNDVLWEISDARRELLAGNREP
                     AFDESQMNAYALADGRLRQIPIEGRKNLEGVKPIWIDLLAASKAERNYVGSHFGLMLP
                     DPNDVTDLEVSSRYLIEETGDIHLHSNFLLDRAGNSRSVPVAFVLHQGILFTLRNEEL
                     PVFRLQRRRAQTQPGYVTDCTDVLLDLYGADVEYSADSLEDIYSTLGKVGRQVLSEAI
                     SDKEAAAILGDIAEEEDLNGRIRGNILDTQRAINFLFRSKIMSSAQQDDARQILGNID
                     SLNSHTSFLFDKINFLMDATIGFININQNRRINQLTIFGVVFTPINILAGMGGMSEFS
                     MMTQGIPWPLAYGGFLAGAGLIGATTYLVLRRLENRQIKIKKGLLAKPP"
     gene            complement(19951..>20639)
                     /locus_tag="PLB25_00510"
     CDS             complement(19951..>20639)
                     /locus_tag="PLB25_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HOE40109.1"
                     /translation="TVLLSPEPMARPLPPAKNAMDWLRGALVAPFMDFVLRYRWQAAL
                     LLALIGTYRISDVVMGIMANPFYVDMGYSKDEVAAVTKVYGVIMTLAGAFIGGALAMR
                     LGVMRVLMLGAVLSAASNLLFATLSTRGHDVSGLILVVSADNLAGGIASAAFIAYLSS
                     LTNVSYSATQYALFSSMMLLLPKFLAGYSGSYVDAFGYPNFFVATALLGIPVLLLVWL
                     ASGIKSGCSP"
BASE COUNT         4223 a         6258 c         6134 g         4024 t
ORIGIN      
        1 gagccgggtg ccacgctggc caataaacag tgctgcctgt tcggcagcgg ccccagcggt
       61 gactgatccc ggcgttgctg cagcaagcgc cggagcctgc cgggcatgac cctttggcat
      121 ttgatcgcgc agcgccagcc agccttgcag tgccagcacc gcctgggccc caaccggaac
      181 aatgcggcgc ttgctgcctt tgcccagcac atgggcttcg ccggcctgca gatcaaccca
      241 gccgcgcgcc tggttgctgg cccgcacatc cagcccggtc agttcggcca gccgcaagcc
      301 cccgccataa agcagctcgg caatcgccgc atcacgtgcc tccagccagg ggtcagccga
      361 ggcgctttga aaactgacca gttgcactgc atcatcgacc cccagcgcct tgggcagtgg
      421 cttggcagcc ttgggcgcac gcaccccttg caccgggttg caggccacca ggccttcgcg
      481 cccaagccag gcgtaaaaac cgcgccagcc cgacaaaatc agcgcaatgc cccggccagt
      541 gcgtccgcca gcatgcattt gcgccaccca gcggcgaatc tgactgcttt gcacctgcaa
      601 caagccaatg ccagccaaag ccgcactgtg cttgagcttt tgcaggtcca gcgtgtaaag
      661 cagcagcgtg cgatcagcca ggcgcttctc aaaacgcgca tggtcgaggt agcgctgtac
      721 cagcgggtct ggtggggcag cgtttgccat ggcttcggtg ctgctggctg gcacgggttt
      781 ggccttcatg aaacagtgtt actcaccggt acttcacctg ggtctgattg aaatcccaga
      841 tgctgcggca aagcgcttgg ccctgcaaga cgacatttct gcaatttccg gccacttcag
      901 cgcaatatgg acagcgcggc actggtcagg tcggcaatac gcgccagaaa atcagtgccc
      961 atggtgctgg caaagcgctg cgcgtcgggt gaggccagca ccagcagacc aaaggcaggt
     1021 gcaatgccgc ccgacaaggc ggcgcccacc ggcgtgctgc gcagcggcag aatcgccagc
     1081 gagacgaccg cctgcggctc gggcagccaa ctgatcgcct ccagaccggt gttgacaccg
     1141 caaaacggct cggtcaacga ggttgcaaac gacttggcat cgtcactgac ccccagcgca
     1201 aacggacagg ccacgtatgc cggtgccaca ccccagacct tgatgccaac ctgtggcacc
     1261 gagaactggt cggcaatccc atcggcaatc aattgcggca aggccagcac atcggtgttg
     1321 agaagcaggc tgcgcgccca cagcagcagt ttgtcggaca acaccatgtt ttcattgacg
     1381 ttgcggatca tgtccatgat gcgttgctcc agcatgcgaa tcttgtcgcg caacagcaat
     1441 gcctgacgct cttgcaggct gacggtgcgc tggttgtgcg ggctggacaa ctgcactgcc
     1501 gccagcagtt cggcgtgtcg cgcaaaaaaa tcaggcgtaa tggccaggta attggcgata
     1561 tcttcttcgg tgatggggct accgattgcg ctggggagtg aggaggggtt gggcatggtc
     1621 ataagctggg ttgaagttca atctggccgt caaaaacggc ggtggcaggg ccggtcagca
     1681 gcaccggggc gcggccacct tgccaggcaa tggtgagttg accgccgtgg gtctggacat
     1741 cgaccgacgc agcaagcagg cccaggcgaa tgcctgccac caccgccgcg caagccccgg
     1801 tgccgcaagc cagcgtttca ccggcaccgc gttcatacac ccgcaagcgg atctgcgagc
     1861 ggctttgcac ctgcatgaag ccgacattga caccgcctgg gaaggcgctc aggctttgta
     1921 tttgcggccc cagcgttgcc accggtgcgc gctccacatc gtccgtcagc agcaccgcat
     1981 gggggtttcc catcgacacc aatgctactt caacgatagc tggacgcgca gtagcatcaa
     2041 gggctagagg ccatttttgc catagacctg aggcctgtgg cagcaagccg gtggcatcaa
     2101 atggcaggtg agccagttca aagtcggggg cctgcatatc gaccgtgacc cggccatcag
     2161 gctgccactg cagttccaat atgcggttca tggtctgcac ccgcaccacg tccttggtgg
     2221 tcaggccctg gcggcggaca aaggccaaaa agcagcgggc accgttgccg cagtgttcga
     2281 cctcacggcc gtcagcgttg tggatcaggt aagaaaaatc gatgcctggc gcgctcgacg
     2341 cgcgcaccgt caaaatctga tcggcaccga cgccgaaatg ccggtcaccc agaaaacggt
     2401 aatctgcagc gctcaggccg tagcgggtcc gggtctcgtc gagcaccaca aagtcattgc
     2461 ccgcgccttg cattttggtg aaagggattt gcatggagga ggattatcta cggcggccgt
     2521 ggcacggcac cggtgccgat acgcaaagac acacccttgc ctggtacctc gaagctggct
     2581 ccacgaccta gctgccttga ctggtcggct gactggcggg ttggctggcg acggcccggc
     2641 ctggctgcag cgccgggtgt ttgctggcat ttttgaccaa cttgcgcgcc accgcgcccg
     2701 gcatgtcaat ggcgcaatgg tccgccacct gcagcaggta caaaaacaca tcggctacct
     2761 catctgcaat gcgctcatgg gtcaccgggt caaggtgcgc ggcttttgat tcatcagccg
     2821 tcatccattg aaaaatctcc agcagctcgg cggcttccac catcagcgct gaagtcaggt
     2881 ttttcggcgt gtgaaagggc tgccagttgc gctcggcggc aaacgtgcgc aagctggctt
     2941 gcagcgcggg gatgtccatc agttgcgccc aggctcgctg cgaccaaagg cgcgccggta
     3001 gagcacccgc agcagcagcg cggccagcag gccaaacagc gccaggctac ccaagccacc
     3061 cagaaacacc acaccccagc cgcgaaactt cgacagcagc cacagcagcg ccaggcaagt
     3121 cagcgcaaag cccgccacat caccgagcgt gcgcacgctg gcgagttcgc tcaatggaaa
     3181 cacctggtga atccccagca cgctcagcag cacacaggcc agtggtgcca gcagcagcaa
     3241 gatattggca tccatcagcc cctggtcaaa agccacatgc gccaaaccca cagccagcag
     3301 tgccgccacc agcagcagca ccgcgccacc aatcacgacg ctggcgatca ggcggccatc
     3361 gtgctggctg cgcccgccct tggcgatgcg cgccagcagc gtgccgccca ggccccagcc
     3421 cagcgcggcc agcagcacgc ccagcgcgtg cgcatcaagc gacagataga gcatgatcag
     3481 gagttcacgc agggacatca ggcttccttg ggggtcagac cggagttggg gtcgggtcag
     3541 tttatcgcag cccagcggcg gcactgtgca ggcgcgcaac gcctgctgcc agcgcctgcc
     3601 agcaacaacc tgcgccagat ggcagcttcg ttgaccatgg cgtttgcgcg tcttgcaggc
     3661 ctgataattt gacccatgcc acgcccatcc caactgctgc acccgccccg tccaccagcc
     3721 acactgcgcc cggccgccac cgtcttgttg ctgcgtgacg gcatggatgg ctttgaagtg
     3781 ctgatgacac ggcgctctgc tgcggccagc tttttgccag gtgcctttgt ctttccgggc
     3841 ggtgccattg atgccgctga cgccaactgc catgccattg cccggcgtcg gccaagccag
     3901 agcgatctgc acctgacgca ggcaatcgct gcgattcgcg agagttttga agagctgggg
     3961 gtgctgctgg cgcatcgggc agacggctcg gcagtcgatc aaggcgacat tgccagactc
     4021 gaccgcagcg catcttttgc tggccaatgc gctgagcagc gcctgacgct agcggcccat
     4081 gaggtgtttg tgctggccca ctggatgggc gaccgcgacc tggcgcgccg ctttgacgtg
     4141 ccatttctgg tggcgcgcat gccagcaggc cagaccccgg tggccgacgg gtccgagcag
     4201 ttcgagccgg tctgggtgcg ccctgctgat gcgctggcac gccacaaggc gggcgacttt
     4261 ttcatcatct tcccgaccat tcgcacgctt gagcggctgc agcactttgc cgatgtggcg
     4321 gcagtgctgc aggcctgcgc cgtgaccgag gagccgctgt ggacctgttg cccgcgcagc
     4381 gggctgctgg gcggccgtga agcacgtttc atggaacacg aagccccgtt tggcgaactt
     4441 gcgctggtgt gcccggacgg acaaatcgtg catccgctgg actggcagag cgagcgcgcc
     4501 gtgccactgc tgaaaaacgt gcagcgcctg accgcaccca accccggtgc catgaccggg
     4561 cctggcacca acagttatct ggtcggcgac ccggccacgg gttacattgc catcgacccc
     4621 ggcccggcag atgccgagca cctcgacaaa ctgtggcgag cagcaggcgg tgatatccgg
     4681 atgattgtgt gcacccattc gcacccggat cacgcccccg gcgcacggcc gctgcaggca
     4741 atgtgccaga ccgccccgcc ggttctgggt cttgccagcg ctgcaacggc gcgcgctgcc
     4801 agcgcttttg cacccgacag aatgctatta aacagagagc tgctgacgct ttcaccacat
     4861 gggctggagg gtgatttggc ttatgaaacc gggcacacgc tgcaagttgt ccacacacct
     4921 ggtcacgccg ccaaccacct gtgcctggta cttttgcaag acggcttgct gttttccggc
     4981 gaccatatcc tcaacggcag caccacggtg attgacccac ccgatggcga catgaatgac
     5041 tacctgggtt cgcttgacgt gttgagcgcg gcctgcgcgc aactgcgcat cgagtacatc
     5101 ctgcctgccc acggttatgt gatcggtggc cccactgatg aggctttcag cgccatcgcc
     5161 aggctgaaaa agcaccgcct gcagcgtgag gccaaagtga tgcaggcgat gcaggcgcag
     5221 ccgcacggct cgctggacga ctggctggca gtggtttatg aggatgtgcc cgagcgcatg
     5281 tggccagtgg cccggcgctc gctgcaagcg catgtcaccc gcattgaatc gatgatcctg
     5341 gattcctccg ctgaccgctt gtttcatctg taagtcctca tgaacattga ctttttcagc
     5401 ttcgatgagg gttcacccgt cgggtcacag cgcgacgcgc ccgattgaaa cactggcaac
     5461 acgactgagg gcgttgctgc tgccgcgtcg gccaggccga catgccagca gtagctgagc
     5521 gtcgtcggca ccccttgcac cttgcacctc gccgcttacc gggccagcca tgccacaagg
     5581 cgggcctgca tcaagtcgtc cgaaggtacg caggcacgtc tggagccgca gcttgcaccc
     5641 tgccctgccc agccgcgccc gccagcagcc gactccggcg cgtccaaccg agcaatctca
     5701 ggatgatggc gtgagctggc gattgaaaat gcacacaccg cgcccaacta ccgggctatt
     5761 gaaaccaacc cgggaaccgc gccatgccgg ctttcatcat tacttgcctg ggtctgctgc
     5821 tggtgttgtg gtttgtcggc cagccatggt ggcttgaacg tcagcgccag acgcggcgcc
     5881 aggcaccctt tccgtctgcc tggcgcgatg tgctgcgcca gcgggttgcg ctgtaccgca
     5941 tcttgccgag ccatttgcag ttgcaactca agcagcacat ccaggttttc ctggcagaaa
     6001 agacctttat cggctgcaat gggctggaaa tcactgatga gatgcgggta acagtcgccg
     6061 cccatgcctg cttgctgatc ctcaatcggc cgggtgacta ctaccccaag ctgcggcaga
     6121 ttctgctcta tccggacagt tttgtggtcg agcgcgatca caccgacggc attggcgtgg
     6181 cgcaccgggc gcgtcaggtg ctggctggcg aatcgtggtc gcaggggcaa gtcatcctgt
     6241 catggcagga cacgctggct ggcgcggccg tggcagatga cgggcaaaac gtggccattc
     6301 atgaatttgc gcatcagctt gaccaggaaa ccggcagtgc caatggtgcg ccactgttgg
     6361 cgcggcgcgg ccagatgcaa agctggtcga atgtgctggg ggcagaattt gccacactgc
     6421 aggcccgtgt gacgcgcggt gcgcccagcc tgtttagcga ctacggcgca accgatccgg
     6481 ccgagttttt cgccgttgtg tctgaagtgt tttttgaaca gccgtcgccg atggcgcagg
     6541 accatccggc gctgtaccag caattgagcg agtactacct gcttgatccc ctgaactggt
     6601 cctgatcgtc acggccaatg gcggcggttt cgcgcaaaaa aagactgcgg ctgtgcggac
     6661 acaagcttgg cgctgtcccc ttggcccttc cgggtgaggg accagcacta ctgccgcacc
     6721 gtggtttcct gatattcaaa gtgaactggc gcgcgatgaa tgagcggcaa aagcagttgg
     6781 cgacggcaag tgtgcggcgc aggccgctat gaccgagggc gcaaccgcac ttgatgccat
     6841 cgacaggaaa gtcttcaaag cgagaaaaac tgccccgaac agttcgatca tctggcgcac
     6901 acgcgcctgg cgtcacctgt cattcaacgg aaacagttac gtgacgctgc gccggatcac
     6961 acccttgttg gcttggcgca tgtgattgac cgttgcgggt ttgctgcggt catgctgctc
     7021 tggcttgcaa gagcacgcca ggcaggcaaa cgcatgaacc agccatccgc agcaaggtgt
     7081 ggtgaatgac aggccgactc ggttccaccg gcgctggaaa aatcatccag cgcgcggttc
     7141 cagcggcgta tttgcgtagg ccactgaatc caggtgccgt gccaatcgca tcgcctgggt
     7201 cggcgatgcg tgagaccgga aaaaaagacc ttagacccgt cgcgcccaca gccccagggt
     7261 ctgccgctcg gcagcggcaa gctcgcgctc gaacagggcg gacctgagca gggaatccgg
     7321 gatcaagccg aaccggacgg ctttggcata gtttgccctg gcgatatcga atggcatgcc
     7381 aaaaatgagg ctggcggtgc gcgcgagggc gctgccggct tttgccaaag tgctttcgtt
     7441 gttcatggtg ggctccttca aggtgtgttc agaccaaagg cgcgacctgg gctggcgtgg
     7501 cggtcacggt gatcaatttc gcgcatgcgc cgttccagat caacggtatc gcaagcttgc
     7561 gacaggtaag caaagtcaag gtcttgctgg gaccggatgc cgggtgaaag cgcttcgata
     7621 agctgagtca gtaggcacat gatgaggccc tttgcgaaag tggaaagatg actcagtata
     7681 agggtttacc ctagctttac aagctaactg gttgttttct ttccacacgc cagaattcgc
     7741 cttgtcgtga tgccagaggc gctgcattgg cgcgggcttg cctgcgttgg cagccacatc
     7801 gacaccgtct tcatcactat gaagtacata gctgtcagcg cttatctcga aagggctgga
     7861 gcctgttttt gccatcaagt caaggcctat tgacggcctc ggtacactgg ttgccagcaa
     7921 gaatcagaaa gtgcaggcaa atgggtatgc aagagcagaa cggcagacca gacaatggcg
     7981 cagcgcgctc tcctggggcg ctgccaacgg ggctgaagtg gggtccgctg ggcattgtgc
     8041 tgttctggct ggtgctgatg ggcttgctgt atgcgttgat gacgcactac ctcaaacccc
     8101 agccagtgcg aattaccgcc agcggcgacc tgatcatccc gaaggcccgc aacgggcatt
     8161 tttatgcggc cggtacggtc aacggccggc cggtgaattt catggtcgat accggggcct
     8221 cgctggtcac ggtcagcgag gcgtttgccc agcaggccgg cgttgccgct ggcgtgccca
     8281 ccgtgttcaa gaccgccaac ggcgatctgc ccgggcgcgt tgtgccggac gtgccggtaa
     8341 cgctcggtcc ggtgcaggtg tcgggcgtgc gtctgggggt cggcctggtc ggtggcgacc
     8401 tcaatgacgg cttgctgggg cagagttttc tgtcgaagtt tgagcttatc gtgacaaaag
     8461 accagatgat tctgcggccg cgctagattg gcttgcacgt ttaactccag gtatccatca
     8521 gcgcagcgcc ctgttcgatc aggaatgcct tgaacgcgcg cgctgccggg gacagctttt
     8581 tgctgctcag atgcgtcaca taccagtggt tgatctgagg caggccttga atgtcgagca
     8641 gcgccagatg gccgctggcg agttcgtggc gaatcgtgcg catcgacaaa aagctcaagc
     8701 ccatgccggc catcactgcc tgcttgatgg tttcgttgct gggcatggcc atcaccacct
     8761 tcatgctggt ctggttctgg gcaaacaggt gctgcatggc ggcacgcgtg cccgagcctt
     8821 cttcgcgcac cacaaagccg tggtcggcca gtgcgctaaa cggcaggttc ttgcgccgtg
     8881 ccaggggatg gtcgggcgca ccaaccatgg ccagcgggtt ggtggcaaag gcggtggctt
     8941 cgcagtccag attgcttggg gcgcggccca tcaccaccag atcggcttcg ttgcgggtca
     9001 gcagccccag cacgttttcg cggttgtcga tgttcaactg cacgtcaatg ccgggcagca
     9061 gcctgccaaa gcgcaccagc aacatcggga tgaaatactt cgaggtgctg accactgcca
     9121 gttcgatgcg accacgctgc aggccgacat gttcggccat cagcgcttcg aggtcccgaa
     9181 actggcccat ggcagcagtt gcatgggcct gaaacgcctg gccggcagcg gtaagccgga
     9241 tgttgcgacc caggggctca accagcgcca ggccaaaggc ttcttcaagc tggcgcagtt
     9301 gcatcgacac cgcaggctgt gtgacaaaca ggcgctcggc ggctttggag accgaacgct
     9361 ggcgggccac ttccagaaat gtctgaattt gcttgaaggt gtaagggcgc ataggtctct
     9421 ccagccgatg atctcatcag caaagcctga tgttttttga ataaaacgtg attggagttt
     9481 atgagtcagc cccattaaag taaacccatg ccgaaaagaa tcggcgattt tcttcaccct
     9541 acaggaccaa gcccatggca aatgctgcac tgaaagcgct gattttcgat gttgatggca
     9601 cactggccga caccgaaagc gcgcacatgg ctgcctttaa ccaggccttt gccgaacagg
     9661 gtctggattg ggtctggacc gatgacatgt acctggagct gctgcatgtc tcaggtggca
     9721 aggaacgcat cctgcattac tggcagcaac gtgaccccgc catcttgcaa gccgacgcgc
     9781 ctgccctgca agcgcagatc aaccgcctgc atgcgctgaa aaccgttgcc tatgagcagg
     9841 cggtcaaccg cggagcggtg cgcttgcgac cgggtgtgct caatctgatt gacgaggcac
     9901 ggcaagccga cctgcaactg gccatcgcca ccaccacctc gccagccaat attgccgcgc
     9961 tgctgcgcca tgcaatgggt gccgactggc gcttgaactt cagcgcgatt ggcgatgctg
    10021 gcaccgcccc gatcaaaaaa ccgcatccgc aggtgtatct gcaagtgctg ggtgcgctca
    10081 aactgcccgc cgcgcagtgt ctggcgctgg aagactcccg caacggcttg caggccgcca
    10141 tggccgctgg catcgccacc atcatcacgc cgaacccatt taccgcgcat cacgatttca
    10201 ctggtgcgct gcgcgttgtg ccggacttga gccagatcag actgaccgac ttgtgcgagt
    10261 ggcagaattc tgcccgggcc tgagccaaaa gaccgtcaaa ccgtttccaa cctagcagaa
    10321 agtcaactca tgagctccag ccccgtcttg cgtcttgccc cgtccatctt atctgccgac
    10381 tttgcccgct tgggcgaaga ggtacgtgcc atcgaggcgg ctggctgcga cctggtgcat
    10441 tttgacgtga tggacaacca ctacgtgccc aatttgacca ttggtccgct ggtgtgcgag
    10501 gcgattcgcc cgcatgtgag cattccgatt gacgtgcatt tgatggtcga gccggttgat
    10561 tcgatcattc cactgtttgc caaagccggt gccaacatca tcacgtttca cccggaagcc
    10621 tcaaaacacg tggaccgcag cctgtcgatg atccgtgagc ttggctgcaa ggctggcctg
    10681 gtgctgaacc cggcttctcc agttgattgg cttgatcacg tgatggacaa gctcgacatg
    10741 gtgctgttga tgagtgtcaa ccccggtttt ggcgggcaga agttcatccc cagcacgctg
    10801 gccaaactgc gcctggtgcg ggccaggatt gatgcccatg tggcagccgg tggccagcca
    10861 atctggctgg aagtcgatgg cggggtcaag actgacaaca tcttcgagat tgtgcaagcg
    10921 ggtgccgaca cctgcgttgc cggcagcgcc gtgtttggcg ccaaagacgc tgacggcggt
    10981 tacaagactg tcatgcaggc gctgcggcgt gctgcccacc cgagctgatt tagagacttt
    11041 aaattaccag gagttttccc atgtccatga cccttcgttc caaccgatcg acgttgacgc
    11101 agtttctgat cgaagagcgc cgccgttttc cgtctgccag cggtgacttc aacgcgctgg
    11161 tgctcgacgt ggcgctggcc tgcaaaggca ttgccaaggc ggtcgcttat ggggaactgg
    11221 ccgggggcac cggcaaagat actgctgact tgggtggcgc gctccatctg ccgggcgaga
    11281 cgcaaaaaaa gctcaaccag ttgtccaacg atgtctttat tcagcgcact gaatggtcgg
    11341 gcaacctcgc tggcatcgct tctgaagaaa tggacttgcc ttaccagata ccaaagcagt
    11401 acccgcgcgg caactatctg atcgtgtttg acccgcttga cggctccagc aacatcgatg
    11461 tcaacatgtc tgtgggcagc atcttcagca tcttgcgggc accccaggaa gtgattgact
    11521 cgggtcgcga tgtcgttgat gccgactttt accagcccgg ctgcaaacag atggcggctg
    11581 gctacgcggt gtatggcccc accaccatgc tggtgctgac ggtgggcgac ggtgtcaacg
    11641 cctttacgct ggacccgaat ctgggtgagt tcatgctgac gcaccccaag ttgcgcattc
    11701 ctgaagatac ccaggaattt gccatcaatg cgtccaacgc gcgcttttgg caagccccgg
    11761 tcaagcgtta tgtcgatgaa tgcctggccg gcaaaaccgg tgtccgtggc aaggacttca
    11821 gcatgcgctg gattgcctcg atggtggctg aggcgcaccg catactgatg cgcggcgggg
    11881 tatttttgta cccccgcgat gccaaggact cggcgcgcgc cgggcggctg cgcctgctgt
    11941 atgaaaccaa tccggtcggc atgatcatgg aacaggcggg tgggcgcgct tcaaccggtc
    12001 aaggcccgat gctgcaagtg caacccactg agctgcatca gcgcatcgga tttgtctttg
    12061 gctcgaaaaa cgaggtcgag cgcattgagc gctatcaccg cgagccggta ctggttgaat
    12121 tgaccaaccc gctgtttgcc gagcgcagcc tttttcggga ttgatgagtc actattaaaa
    12181 atatagctgc tcgcgcatat cccataagcg ctagagccca aatacttgtc aatatttaac
    12241 ttcaggagtg tttgccatgt caattcgcca ccccatcatc gccatcaccg gctccagcgg
    12301 cgctggcacc acctcggtga cccgcacctt tgacaacatt tttcgccgtg aaaacgtcaa
    12361 ggccgcgatc attgaaggcg acagctttca ccgccacgac cgcaagtcga tgaaaatcgc
    12421 cgccgccgag gctgaagccg ctggcaacca caacttcagc cactttggcc tggaaaacaa
    12481 cctgttttcc gaactcgaga cgctgtttgc cacctacgcg aagaccggca ccggcaagcg
    12541 ccgcaaatac ctgcacgatg cggaagaggc cgcgccttac aagcaagacc ccggcacctt
    12601 cacgccatgg gaagatgtgc ccagcggtac tgatttgctg ttttacgagg gcctgcatgg
    12661 cgcggtcgtg acacccgaga tcaacatcgc gcagcacccc gatctgctga ttggggtggt
    12721 gccggtgatc aacctggagt ggatccagaa gctttaccgt gacaaaaagc agcgcggcta
    12781 cagcaccgaa gcggtgaccg acaccatctt gcgccgcatg ccagactatg tgaactacat
    12841 ctgcccgcag ttcatgcaca cgcatgtcaa tttccagcgc gtgccgatgg tcgataccag
    12901 caacccgttc atctcgcgcg acattccgag tgccgacgaa agcatggtgg tgatccgttt
    12961 cgccaacccc aagggcattg actttcagta cctgcgcaac atgatcaacg acagttacat
    13021 gagtcgcgcc aacaccatcg tggtgccagg cggcaagatg gaactggcca tgcaactgat
    13081 cttcacgcca tttgtctggc gcatgatgga gcgccagaaa aaggcttcct gatatattgt
    13141 gggaaagacc aagttacatg agcgaagcga atgaacttgc tctgtcccca actgactaga
    13201 aaattgtggg aaagaccaag ttacatgagc gaagcgaatg aacttgctct gtccccaact
    13261 gactagaaaa ttgtgggaaa gaccaggtta catgagcgaa gcgaatgaac ttgctctgtc
    13321 cccaacaaat cagagaattg tgggaaagcc cgagttaccc gcgcgaaacg tatgaacttg
    13381 cccggtccca acacattcac ccttaaccca atcaatttcg aatcatgaca acccctaccc
    13441 ctgaacttgc caaacaaatg gccaatgcca tccgcatgct tgccgttgac gcggttgaaa
    13501 aagccaagtc cggccatccc ggcgcgccaa tgggcatggc cgagatcgct gaagtgctgt
    13561 ggaaccggca tctgagtcac aacccgatca acccgcactg ggccaaccgc gaccggtttg
    13621 tcttgagcaa cggccacggc tcgatgctga tttatgcgct gctgcatctc accggctatg
    13681 acttgccgat gagcgaactg caaaacttcc gccagctcca cagcaaaacc gccggtcatc
    13741 cggaatacgg catgacccct ggtgtcgaga ccaccaccgg cccgctcggc cagggcgtgg
    13801 ccaacgcggt cggcatggca ctggctgaaa agctgctggc cgtcgaattc aaccgcgaag
    13861 caaacggtgc ccgccacacc atcgtcgatc acttcaccta tgtgtttttg ggcgacggct
    13921 gcctgatgga gggcatcagc cacgaagcct gctcgctggc cggtacgctg cgcctgtcga
    13981 aactggtggc gttttatgac gacaacggca tttccatcga tggtcatgtc gaaggctggt
    14041 tcaccgacga cacccccaaa cgctttgaag cctatggctg gaacgtgatt gccgcagtgg
    14101 atggccacaa cccggccgca atcgatgcag ccatcaaggc ggcccgggcg caagcggtgt
    14161 tgcccgatgg tcggccgacc ctgatttgct gcaagacggt gattggcaag ggctcgccca
    14221 acaaggccgg tagccatgat gtccatggcg cggcactcgg tgctgccgag gtagcggcag
    14281 tccgtgatga gctgaactgg gtcagcgcgc cgtttgagat tcccgctgac attgctgccg
    14341 cctggaatgc cgtggcgcgc ggtgtggtgg ctgaacacgc ctggcgcgtt cgctttgatg
    14401 cctacagcgc cgtttaccct gcccaggcag ccgagtttga gcgccgcatg gcgggcaatc
    14461 tgttgccaga cttcgccgac aagctgcctg gtttgctgga aaccattgcc gccaatacaa
    14521 cggcatttgc cacccgcaaa gccagccaga acgcgcttga cgtgattgcc ccgctggtgc
    14581 cggagttctt tggtggcagt gccgacctga ccggctcgaa cctgaccaat ttcaagggct
    14641 gcgtgcgcgc cgggcgcgat gtctggggca accacctgag ctatggtgtg cgtgaatttg
    14701 gcatggcggc catcatgaat ggcatggcgc tgcatggcgg cttcatcccc tacggtggca
    14761 ccttcctgac gttttccgac tacagccgca acgcgatccg catggccgcg ctgatgaaaa
    14821 tccgtgtcat ccatgtgttc acgcacgaca gcattggcct gggcgaagac ggcccgacgc
    14881 accagagcat tgagcacatc ccctcgatgc gcatcattcc ggggcttgat gtgtggcgtc
    14941 cggctgacgg tctggaaact gcggtggcat gggcttttgc ggtcgagcgt cgtgacggcc
    15001 ccagcgccct gtgcctgtcg cgccagaacc tgccgcgtct gaccgatatg tcgatggctg
    15061 tgaacatccg caagggcggc tacatcctgt cagacatgca agacgcgcaa gccgtcatcg
    15121 tctccactgg ctccgagctg gaaattgcca tgaaggcgca ggcattgctg gccgccgatg
    15181 gcattgccac ccgggtcgtg tcaatgccct gcaccaatgt gtttgaccgc cagagcaatg
    15241 cctaccagga gatggtgatc ccgttcaaca cgccctcggt ggcgatcgag gcggcacacc
    15301 cggacttctg gcgcaagtat gtgggccgtc acggcgtggt gatcggcatg cccacctttg
    15361 gcgagtcggc cccagcgccc aagctgtatg cacacttcgg catcacggca gagcgggtgg
    15421 tgagcaccgt tcgcaccatc gtgcaccgcg cggccaacaa acgcgacgat gcactgccag
    15481 acaccgtggt gccttcggac aattgaactg gatgccaccc atgaagactt ttgatctggt
    15541 catgtttgac ctggatggta cgctggtgga aactgcgccc gaaatcatgg acgctgtcaa
    15601 tgacaccttg ggtcacttcg atttgccgct ggtgtcacag cagcaggtga acaactggat
    15661 tggtcacggc accttgacgc tgctggttga tgcggtagcc tttgcgaccg gcaggtcggc
    15721 ggccgatgtg cgcgccagcg agctgctgca aagcatggtg caagagtaca acctgtttta
    15781 ccagcgccgc tgcggcaccc gcagccattt gtacccgcag gtgcgagaga ccctggtggc
    15841 cctgcgcgca cagggcgttc ggctggcggt ggtcaccaac aaggaaagcc gctacaccca
    15901 gatcgtgatg gacgtgcaag acctcagcgc gttgttcgac acggtgatca gtggcgacag
    15961 cttgccgacc aaaaagcctg acccggcggg tgtggcgcac tgcctggcac agtggggcgt
    16021 tagcgctgac cgtgccctgt ttgttggcga ctccagcatc gatgtcgcca ccgcccgcaa
    16081 cgccggtgtc gcggtgtggg ccgtacccta tggctacaac atgggccagc cgattgaagc
    16141 ctgcacaccg gaccgtatca tcgcggactg ctctgcgcta ttgaatagct agctattcgc
    16201 gcagtaccat cacgcgctcg gtgtctgttt ttcattaact tttggagttt tccgatgaaa
    16261 gtattgcgtt ttgctgatgt gattgccagt ggtgctgcca agggcaaaag ggtgtttatc
    16321 cgtgccgact tgaatgtgcc gcaggacgat gcaggcaacg tcaccgagga cacccgcatt
    16381 cgcgccagtg tgccgtgcat caagatggca ctcgacgcag gtgcagccgt gatggtgacc
    16441 agccacctgg gtcgccccac agaaggcacc ttgacacccg aggattcgct ggccccggtg
    16501 gccaagcgca tgggcgaact gctgggccgc gaggtgccgg tggtggccaa ctgggtcaac
    16561 ggcgttgatg tcaagcccgg ccaactggtg atgctggaaa actgccgcgt caacaagggc
    16621 gaaaagaaga acaacgaaga gctggcgaaa aaaatggccg ctctgtgcga cgtgtttgtg
    16681 cacgatgcgt ttggcacggc gcaccgggcc gaaggcacca cctacggtat tgcccagttt
    16741 gcaccgattg cctgcgccgg cccgttgctg gctgcagaaa ttgatgccat caacctggcg
    16801 ctggccaacc cgaaacgccc cctggtggcc atcgttgcgg gctccaaggt atcgaccaaa
    16861 ctcagtatct tgcaggccct ggccaagaac gtggacaacc tgatcgtcgg cggcggcatt
    16921 gccaacactt tcatgctggc tgctggcctg aaaattggca agagcctggc cgagcccagc
    16981 ctgctggctg atgcggttgc cgtcatagct gccatgaagg cgcgcggtgc cgatgtgccg
    17041 atcccgaccg acgtggtcac cgccaagact tttgccatcg atgccgtggc caccgtcaag
    17101 gccgccaccg ctgtcgaaga cgacgacatg attctggaca ttggcccgga caccgctgcc
    17161 aaactggcca agcagctcat ggaagccggc accattgtct ggaacggccc ggtcggcgtg
    17221 ttcgagttcc cgtcgttcga gaatggcacc aagacgattg cgcaggcgat tgctgcgtcc
    17281 agcgcgttct cgattgctgg cggcggcgac acactggccg ccattgccaa gtacggtatc
    17341 gaaaaagaca tcggctatat ctcgactggc ggcggcgctt tcctcgaagt gctggaaggc
    17401 aaaacgctgc cagccttcga gattctgcag aaacgggcgg cgggctaagc ccgaagccca
    17461 agtccctgcc ctggcgcagg cgcaggcagc agaaagcggc agggacttgt accagcgttt
    17521 tcctcatgta cattgaggca gcgctggtgc tcaatgttcg gccataaaaa gaggctccag
    17581 cctttgtcag acgggcgcaa gcagctatta aaatgatatt aaacgctgaa gcgcctgcgg
    17641 ccttggcacc agccactcgc tgatgccttg agccgtgcgg gtaatgggcg agccccgcag
    17701 ttgccgggat tgtgcaaacc tcagatcagc agttttgccc aagctggcca caaacgttga
    17761 aactggccac caagactcat tcccattgaa ggccggacag gttctttact ggcctgtact
    17821 gaactgcctg cagcacgccc gaatggcaat gcggcaacgg agttgtcaga ttttcagtgg
    17881 ctttgaacct cgtgcttggt ctctaacaag tcaagtcttt gcatatcatg cgaagaattc
    17941 aaacattcaa agtcattcgc cgcgagggcg cgactcctac aaattcaaat aggcgccgac
    18001 tgtctggtag gagttgcgcc ctcgcggcga aggcggttca attccggctc gtccggctta
    18061 ggttcatggc ggttttgcca gaaggccttt cttgatcttg atttggcggt tttccaggcg
    18121 gcgcagcacc aggtaggtgg ttgcgccgat cagccccgcg ccggccagaa acccgccata
    18181 ggccaatggc catggaatgc cctgcgtcat catggaaaac tcggacattc cacccatccc
    18241 ggcaagaata ttgatggggg tgaacaccac accaaatatg gtcaactggt tgattctgcg
    18301 gttctggttg atgttgatga aaccgatggt ggcatccatc aggaagttga ttttgtcaaa
    18361 caaaaacgag gtgtgactgt tcagcgagtc aatgttgccc agaatctgcc tggcatcatc
    18421 ctgttgcgca gacgacatga ttttgctgcg gaaaaggaaa ttgatggcgc gctgggtgtc
    18481 cagaatattg ccccggatgc ggccgttgag atcttcttct tcggcaatgt cacccagaat
    18541 ggctgcggcc tctttgtcac tgatggcctc gctcaaaacc tggcgtccca ctttgcccaa
    18601 ggtgctgtaa atgtcctcca gcgagtcagc cgagtactca acatcggctc catacaggtc
    18661 aagcaacaca tctgtgcagt cggtgacgta gcccggttgg gtctgggcgc gccgacgctg
    18721 caaacggaaa accggcaact cttcatttcg caaggtaaag agaatgcctt ggtgtaaaac
    18781 gaacgccacc ggaacactgc gcgagttgcc cgcgcggtcg agtaaaaaat tcgagtgcag
    18841 gtgaatgtca ccagtttcct caatgaggta acgtgaactg acctccaggt cggtcacgtc
    18901 gttcggatca ggcagcatca ggccaaaatg cgacccgacg taatttctct cggctttgct
    18961 ggccgccagc aggtcaatcc agatcggttt gacaccctcc aggtttttgc ggccttcgat
    19021 gggaatttgt cgcagtctgc catcagcaag cgcataggcg ttcatctgac tctcgtcaaa
    19081 tgccggctcg cgattgccgg ccaacaactc ccggcgcgcg tctgaaattt cccacagcac
    19141 atcgttttgc ctggccaaag gaatttgcgc ccacaacagc cgggcatcgt caatcggcaa
    19201 ggcatcaaga acgctgccaa tttcgctggc tggccattgg gccagcagat tttgcagctc
    19261 tgccagatgt tgccgctgaa ccaaagtctc gacgattgcc tggcgcggca tgttctggct
    19321 gtgaaccatg ccctcaaccc gcttttgctt cttaagcaac tccaacactt tttcaaggga
    19381 catggtcatg gcgctcggtt agtggtgaat gcgccagctc ttgatgccaa tccgggggcg
    19441 caacaaacgc aagcgacagg attggcaggc gcgacaggtt caggtgcatc actgccgctc
    19501 accagcctga caccagtcgc gatcaaatgc caagccatgc gtcggtcagg cgtgtcgcat
    19561 ggtatcggtt ggctcgggtg ctttatgggc atcaaggttt gttttggcca acgcaaatgg
    19621 caaattggcc gctgcgaaac accctgccga tggtctgagt ttccagacat ttcaggcttc
    19681 tcacaaagga aaatgcgcgc atggcaacaa atgatagcca ccgttgatga cttcagatgc
    19741 ctgcattggg gaagctaccg aacaagcgac agtcaatcgc ggcagtggtc cggccgcctt
    19801 ctcactttct tgcactgttg cgcgcatgca gggaaagccg tttgccaaaa taaggcgatg
    19861 gcgaccttga cttgtcggca agtcgctgca aagcgaatgg cattcggcgc tcatgttact
    19921 attgttttag aagcatctag cgcacaccag tcaagggctg cagcctgatt tgatgcctga
    19981 agccagccaa accagcagca gcaccggaat acccagcaat gcggtcgcaa caaaaaagtt
    20041 cgggtaacca aaggcatcta cataactccc cgagtagccc gccaggaact tcggtaacag
    20101 tagcatcata gaactgaaca gggcgtattg ggtggccgaa taactgacgt tggtcaggct
    20161 cgacaggtag gcgataaagg ccgccgacgc gatgccaccg gcgaggttgt ctgccgagac
    20221 cacaaggatc aggccactca cgtcatggcc gcgcgtgctc agggtggcaa acagcaggtt
    20281 gctggcagca ctgagcacgg caccgagcat cagcacccgc atgacaccca ggcgcatcgc
    20341 caatgcacca ccgataaagg caccggccag cgtcatgatg accccgtaaa ccttggtcac
    20401 cgctgccacc tcgtccttgc tgtagcccat atccacgtag aaggggttcg ccatgatgcc
    20461 catcaccacg tcgctgatgc ggtaggtgcc gatcagtgcc agaagcagcg ccgcctgcca
    20521 gcggtagcgc aacacaaagt ccatgaacgg tgccaccagc gcaccgcgca gccagtccat
    20581 cgcgttcttg gccggcggca gcggtcgcgc catcggttcg ggcgacaaca gcacggtca
//
LOCUS       DAOSLL010000006        20505 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_2853_length_20505_cov_4.801174, whole genome shotgun sequence.
ACCESSION   DAOSLL010000006 DAOSLL010000000
VERSION     DAOSLL010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 20505)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20505)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20505
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2853_length_20505_cov_4.801174"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..6016)
                     /locus_tag="PLB25_00515"
     CDS             complement(<1..6016)
                     /locus_tag="PLB25_00515"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HOE40110.1"
                     /translation="MPAISFTRKLRHALAMGFVGLLLTISAAGAHAATTTVAIGFDTD
                     NNAATGCTLTSGSSSMPGVETVLYTVVTTAVNTGTVSTITRSICIAGVFGAPINVSAG
                     GWPVGMSAGLNGSDLIETFIPLADLGGTSSVKMGAITPSSDSLIAAGVFAVSPLAPTA
                     PEPIPSLSPTGMLILLSLIGSTGWLMRRHAREGGKLLLLSCLVAGTLSTVSALAIVLD
                     GNGVDWTGIAPLATDSAVDAPAGQDLVALYAIQDGANLALRVDMVLAREAGNQQPEVN
                     AGGNQTIALAAVATLSGSATDDGLPNPPATLTYAWSKVSGPGTVTFGNAASAATSASF
                     SLAGAYVLRLSASDSVQTGVGEVAITVTPGVSLNQAPSITQAAVTVVLPQSLDLDPAV
                     TDDGLPNPPGGLTYRWTQVSGPAVLPPAPSTAFAPVGFNSTTTARTTVSFDPVGPGAY
                     VLRLVASDGELETSHDIAVTVLAQPLAAPAFSPLPTQTVRLGDTLSVALPGNDINPRD
                     TLTYSLLVAPTGAALVPTGSSRFTFTPSAGQVGTHLVTVQVRDAVGSTAQASFSVIVQ
                     NANRTPLFTAASKANAAASVGTTYARTLVAIDPDIGDSLTYALISGPAGLTISASGEL
                     AWSPSPSQRGDHIIKAKVSDVAGAVDVAMFTLTTGNNVAPLARDDVYEVRLGETLTIP
                     AAGVLGNDLSLSGNPLTAAKLSDPDKGTINAFNADGGFNYTAPATLPAVPGLNPVVSW
                     RAVGDGNDHFVLAADVDHDDVVEYVLSALGDFRAKRGSNGSQLWQWDRSITTHANVSA
                     CGTATDEFALGNVSDVVGGDIYLFSSINCDQGGYAGYPDRLFAVNASQILTGGKVATQ
                     WKSERLSRPHPGAYATASSLAPADPPITPFFGASADGSVPTLAKLTAGGSTKLLTRFL
                     ATDVYGPYYDTPNSNHFGRAACRTVTGLPADEGRACKATFVIDAATGAIDQVLTAPNA
                     ANQVNTYVPQGTPTKQNVPIVADLDGDGQVEIISGGDVWKLAGGVWTLAWQAEFASVT
                     SGVKLGFEPTSVAVADLDGDGKAEVIIHIIGYDNGPQKNAGGIHIFNHDGTLRRTIPI
                     PFTWINGLLSVADVDGDGAPEVLLAGDSFVYAYRQDGSLLWAKLPPDVISDVVPAIAP
                     IAMPRTTDSPLYVYDLNLDGVPEVILQSTRRLFILNGRTGAELWSIDTESERYYLHGN
                     PLLVDADNDGHVDILVHVANRWNCDYWGSGPGGCKGNAMRISGGDNNWAPGPKVQNQL
                     NFRPTAINDTAQILYDASVRRDFRQQIQQGTVIDPRIAQSTAFTYKANDGAADSAPAI
                     VAIDIKPPNRPPVITSIPPSALLRASPDGVKTIYTITAVDPDVGDTVHFELVTSSFDA
                     TSYPGPTVDAVTGNLNLYSCRACGTQRIVITVAAVDTQGARTEQSFLIDLSPTAVAVP
                     NVIGQLLPDASTAIEAASLKPLVVTELFGPQAAGTVFGQDPLAGAANVGRGATVKLTV
                     SKGPQPVLVPNVVGAVETAAATALANAGFSVIVARQFSTVIAAGLVISQSPTPGTMLP
                     PGTAQIIVSSGTGLGLKLDRGLMSANGAIVVTPVAIDVDGNTVATPVLTYAITPRQLP
                     FAGTLPTMAGLTITAGLTTMGSFVVTATDAANSRTAAAEFAVALPADADGGGSSASFA
                     RLLSVLQGIDDFGPALRAARAANDVPQMKVLLGQMVTLWRTVDLDDLKDSMVLVFAQG
                     FAPSRRDLDALGLSPTDDDLLVKQVMRDAVADLDAWTQGLRGTTASLSDLNALADQFI
                     SRSARINALTVSEYGVVLSQPEYQMLVAQRIPAFYEAVMEEAAELAGIPRRNSPFPYQ
                     KRMTVAGAASQVTMPQIKSTLVELAVTQAVGYITDKIIEKGVETFANGKKFAVDVMGQ
                     ASWGAGAMVIASHLRDLTYGSEIIATVAGASMSFHLFEAPYSFIEVISNDEPSLNDVM
                     IIGKDITDEAVAAVTGLLDAMKEGFSFGKDAATNPKRYRNQDEAQKT"
     gene            7260..8249
                     /locus_tag="PLB25_00520"
     CDS             7260..8249
                     /locus_tag="PLB25_00520"
                     /inference="COORDINATES: protein motif:HMM:NF013335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HOE40111.1"
                     /translation="MAMTPPRKLIFDTDPGVDDAMALCYALAHPGLDVIGITTTFGNV
                     TVEQASANALYLCALAGRSIAVAQGVARPMVKQAESPPDFIHGADGLGNLPERHHCSA
                     QVDSRSAAQFIVDTARQSPGDITLVAVGPLGNLAAALQLDPELPKLVREVVLMGGTVI
                     ESGNVSPVAEANIWNDPHAADIVFTAGWELTMVGLDVTHQVILPVALFEQIAAHHCHA
                     ATDALAHAVRFYSAFYSQHDPLAGQINGCYGHDVLAFVVLTDPDLFTLQRGRVRVAVD
                     GLAQGQTMMQRKAVSYPQHGWHDDIPHTQVCLQVQAQACREVIEQTLMRRWLG"
     gene            complement(8340..9710)
                     /locus_tag="PLB25_00525"
     CDS             complement(8340..9710)
                     /locus_tag="PLB25_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HOE40112.1"
                     /translation="MSKQTKFLLNEDQMPKAWYNLQADFPKPLPTVLHPGTMQPVGPD
                     DLAPLFPMSLIMQEVSLEREIEIPEPVRDIYRLWRPAPMFRAHRLEKILGTPAKIFYK
                     YEGVSPAGSHKPNTAVPQAFFNKEAGVKRLVTETGAGQWGTSLAFAGSLYDMEVEVFQ
                     VRVSYDQKPYRRAVMETYGARCVASPSNETVYGRSVLAKRPDHPGSLGIAISEAVELA
                     VQREDTKYALGSVLNHVLLHQTIIGQEAMQQMEMADTWPDVIVGCTGGGSNFAGIAFP
                     FIGHGLRGGPKPRMVAVEPAACPSLTRGKYAYDFGDTGHLTPLTKMHTLGSQFTPPGF
                     HAGGLRYHGMAPMVSHCKQLGLLEAVSYNQVECFEAGVMFARAEGIVPAPEANHAVRG
                     AIVEALRCKREGKSETILFNLCGHGHFDMAAYQKYFAGELTDEKYDESELAMALAGLP
                     SVPDTV"
     gene            complement(9807..10511)
                     /gene="rpiA"
                     /locus_tag="PLB25_00530"
     CDS             complement(9807..10511)
                     /gene="rpiA"
                     /locus_tag="PLB25_00530"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HOE40113.1"
                     /translation="MKQVQTAAAASLSQDDLKTLVGQAALHYVAAGEVVGVGTGSTVN
                     KFIDALASMKDQIKGAVSSSEASTERLRALGIAVFDCNEVAELSVYIDGADEIDHHGN
                     MLKGGGAALTREKIVAAQSRIFVCIADDSKLVQTLGRFPLPVEVIPMAAQRIKRQFEA
                     MAARAVLRLKDGKPLVTDNGQHILDVTGLQIADPLAFESLVSQWPGVVTVGVFAHQKA
                     QVCLLGTASGVKTLVF"
     gene            10559..11653
                     /locus_tag="PLB25_00535"
     CDS             10559..11653
                     /locus_tag="PLB25_00535"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HOE40114.1"
                     /translation="MALVPYPLARPFLFGLDPEVAHELTLGALARTQGSVLQLAYASV
                     RVDDPREVAGLIFPNRVGLAAGLDKNARCIDGLGAMGFGFVEVGTVTPLAQGGNPKPR
                     MFRLPQARALINRLGFNNEGLEAFVANVKQSALYLRRQGGDDESDPMLLGLNIGKNAA
                     TPIERATDDYLTCLDGVYPYADYVTVNISSPNTKNLRSLQSDKALDALLGAVSERREL
                     LAQRHGKHIPVFLKIAPDLDADQLEVIARTLQRYGTDRTGRANNAWGVIATNTTLSRE
                     AVVGMAHAQETGGLSGAPVLAMSNQVIRQLRAALGKDFPIIGAGGIMSAADAVSKIAA
                     GADVVQLYTGLIYQGPGLVRKAALMIKNTF"
     gene            complement(11682..12788)
                     /locus_tag="PLB25_00540"
     CDS             complement(11682..12788)
                     /locus_tag="PLB25_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOE40115.1"
                     /translation="MEQVDCVVVGAGVVGLAVARALAQSGREVLALEAANAIGTGTSS
                     RNSEVIHAGICYPQDSLKARLCVQGRAMLYDYCAARHIAHQRCGKLIVATSVAQVGQL
                     KLIADQARRNGVDDLVLLSQQQALTMEPQLRCQGALLSPGTGIVDSHGLMLSLQGELE
                     SAGGVVALNSPLALMEYAQGAIKLRTSDGTRLRVRLLVNAAGLQAPDVARRFKGVPAQ
                     LVPQAFYAKGNYFTLAGRAPFSHLIYPLPEAAGLGVHLTLDLGGQAKFGPDVQWVAAP
                     DDLTVDPARGDAFYAEVRKYWPALPDGALLPGYAGIRPKISGPNAAAADFLIQGPQDH
                     GVPGLVNLFGIESPGLTSALAIGQAVVKILVTTK"
     gene            complement(12871..13635)
                     /locus_tag="PLB25_00545"
     CDS             complement(12871..13635)
                     /locus_tag="PLB25_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40116.1"
                     /translation="MAIHLYFSLIPEALIASMLPPEEFGQYYATGHKFKSKGQAVFFE
                     IDPSYRHAYFDIDDAFAKCVAKPDGSPKNSVYISMYRVMEHLTMASLGKLYLSTANGA
                     TLGLSRASEVPQPVPELHMYQDLAPINSLVVSRLDPAFFCSDVTTTPSKFFRFPGLCF
                     VELELGPLARDPENGQEGDLPYSFMQHLREALMELKPGGKRNKLVHRVHSLVFPFRMV
                     KGGFYVGLDSEFVHYPMPSQALLRKDHSNWWRSANL"
     gene            complement(13760..16264)
                     /locus_tag="PLB25_00550"
     CDS             complement(13760..16264)
                     /locus_tag="PLB25_00550"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOE40117.1"
                     /translation="MKFGNSSIESQWFGVARRLLMLLLLSIGAGSALAASGHTSEVDL
                     VLPNLGDTALISFLGGMSGSTLLSYGLLVCLGGLIFGAVIYRQIRDLPVHRTMAEVSE
                     LIYETCKTYMFTQGKFLLILQACTAVLMIGYFYFVQHLAVSDVALILAFSLIGIAGSF
                     AVAWFGIRINTLANSRTAFASLAAKPFPVYAIPMKSGISIGMLLISTELLIMLCILLF
                     VSPAMAGKCFIGFAIGESLGAAALRIAGGIFTKIADIGSDLMKIEFGIQEDDARNPGV
                     IADCVGDNAGDSVGPTADGFETYGVTGVALITFIMLAIPEPAVQVQLLVWLFVMRVMM
                     IVASGVSYFGNDLLARALYGDKPRFNFEAPLTSLVWITSVVSLILTFIVSYLLMADFA
                     VAGKAYANLWWQLSLIITLGTLAGAIIPEVVKVFTSTHSKHVREVVTASKAGGASLNI
                     LSGIVAGYFSAFWIGVIIVGLMSGAYIVSQFDLTAVINPDHTKAVMMAAVFSFGLVAF
                     GFLGMGPVTIAVDSYGPVTDNAQSVYELSLIEQEPGIEAEVKRDFGFNLDFRAAKFLL
                     EENDGCGNTFKATAKPVLIGTAVVGATTMVFSIVMLLTLGLTDNLDKLSLLHPPFLLG
                     LVSGGATIFWFSGASTQAVSTGAYRAVEFISTHIRLDAVVKASMKDSKKVVEICTIYA
                     QKGTINIFMAVFFGTLACAFIEPFFFIGYLISLAIIGLYQAVFMANAGGAWDNAKKIV
                     ETELHAKGTDLHNASVVGDTVGDPFKDTASVAMNPIIKFTTLFGLLAVEMAVGLNAQG
                     HQSVVYGLAAIFLAVNLVFVFRSFYGMRIDSKETAA"
     gene            16586..17026
                     /locus_tag="PLB25_00555"
     CDS             16586..17026
                     /locus_tag="PLB25_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOE40118.1"
                     /translation="MSRILIRLPETFPFCTDIVLYQSHINYGGHLDNALLLTLVSEAR
                     VRFFKSLGYTELDVEGVGIIVSDAALQYRSEAFHGEVMLVRIGPANFSRKGCDLLWCM
                     NEQSSGREVARGKTGIVFFDYATHKTVPVPQAFRDRHPADEPFQ"
     gene            complement(17748..19004)
                     /locus_tag="PLB25_00560"
     CDS             complement(17748..19004)
                     /locus_tag="PLB25_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03862 family flavoprotein"
                     /protein_id="HOE40119.1"
                     /translation="MKKIIVVGAGPAGLMAAEVLAKAGMVVDVHDAMPSVGRKFLLAG
                     KGGLNLTHSEPENVFVSRYGARREQMETTFQGFHPQDVRSWACSLGIETCVGSSGRVF
                     PADMKAAPLLRAWLHRLRHPQAGHAVQFHMRNRWTGWHSPAGAPTRLDFDTPTGPTQV
                     DADAVVLALGGGSWARLGSDGAWVPWLRSSGVAVSPLLPANCGFDRLGGWTDHFANRF
                     AGLPFKSVAIAMTSHLGEPFHRKGEFVVTATGVEGSLIYAASKLLRDDILVQGQATFY
                     LDLLPDLSVQRVLAEVCHPRGSRSLSSHLKSRLHISGIKMAILHELLSKEQLHDPVRL
                     AAAIKAIPVTLSATRPLDEAISSAGGVVFEAMDDNLMLTAMPGVFCAGEMLDWEAPTG
                     GYLMTACLATGVRAGQGVLKYLQRAA"
     gene            19427..19759
                     /gene="trxA"
                     /locus_tag="PLB25_00565"
     CDS             19427..19759
                     /gene="trxA"
                     /locus_tag="PLB25_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HOE40120.1"
                     /translation="MASELIKHVSDASFEGDVLKSELPVLVDYWAEWCGPCKMIAPIL
                     DEVSADYQGKLQVAKMNVDDNRDVPAKFGIRGIPTLMLFKNGQLAATKVGAMSKSQLI
                     TFIDQQLA"
     gene            19962..>20505
                     /locus_tag="PLB25_00570"
     CDS             19962..>20505
                     /locus_tag="PLB25_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HOE40121.1"
                     /translation="MHLNELKALHVSEVLKQAEDLEIENTGRMRKQELMFAIIKKRAR
                     AGEQIFADGVLEILPDGFGFLRSPDTSYTASTDDIYISPSQVRRFNLHTGDMIEGEVR
                     TPKDGERYFALNKLEKVNGGGPEENKHKVMFENLTPLFPKEQMRLEREIKGEENITGR
                     IIDIISPIGKGQRALLVAPPK"
BASE COUNT         4465 a         6196 c         5926 g         3918 t
ORIGIN      
        1 tggtcttctg agcctcatcc tgattgcggt atcgcttcgg attggtggcc gcgtccttgc
       61 caaagctgaa gccttccttc atggcatcga gcagccctgt caccgcagcg actgcctcgt
      121 cggtgatgtc cttaccgatg atcatcacgt cgttcaggct cggctcgtcg ttcgagatca
      181 cttcgatgaa actataaggt gcctcgaaca ggtggaacga catcgacgca cctgccacgg
      241 tcgcgatgat ttccgatccg taggtcaagt cgcgcaggtg cgacgcaatg accatcgcgc
      301 cagcccccca ggaagcctgc cccatcacgt cgacggcaaa tttcttgccg ttggcaaagg
      361 tttccactcc cttttcgatg atcttgtcgg tgatgtagcc aaccgcctgc gtcaccgcca
      421 actcgaccag cgtcgacttg atctgaggca tcgtgacttg cgaggcggca ccggccacgg
      481 tcatgcgttt ctggtacggg aatggcgaat tgcggcgcgg aattccggcc aactcggcag
      541 cctcctccat caccgcctcg tagaaggccg gtatccgctg cgcgaccagc atctgatact
      601 ccggctgcga cagcacgaca ccatactcgc tgacggtcag cgcattgatg cgcgctgatc
      661 ggctgatgaa ctggtccgcc agggcgttga gatcggacag ggaggcggtt gtgccgcgca
      721 gtccttgcgt ccaggcatca aggtcggcga ccgcgtcgcg catcacctgt ttcaccagca
      781 ggtcgtcgtc ggtcgggctc aaccccaagg catccaggtc gcggcgcgac ggcgcgaagc
      841 cctgcgcaaa gacgaggacc atcgaatcct tgagatcgtc gagatcgacg gtgcgccaca
      901 gtgtgaccat ctgccccaga aggaccttca tttgcggcac gtcgttggca gcgcgcgcag
      961 cgcgtagcgc gggaccaaaa tcgtcgattc cctgcagaac ggaaagcagc cgagcaaaac
     1021 tggcgctgct gcccccgccg tccgcatcgg cgggcagggc gacggcgaat tccgcagccg
     1081 cagtgcgcga gttcgccgca tcggtcgctg tgacgacgaa cgagcccatc gtcgttaaac
     1141 cggccgttat cgtcaggccc gccatagtcg gcagcgtgcc cgcgaacgga agctgcctgg
     1201 gcgtgatggc gtaggtcagg accggggttg ccaccgtgtt gccatcgaca tcaatggcga
     1261 ccggggtcac aacgatcgcg ccgttcgccg acatcaagcc acggtccaat ttcaacccca
     1321 gcccggtgcc gcttgacaca atgatctgcg cggtgcccgg tggcagcatc gtgccgggcg
     1381 tcggcgactg cgagatcacc aggcccgctg ctatcaccgt cgagaactgc cgtgccacga
     1441 tgaccgagaa cccggcgttt gcaagtgcgg tggcagccgc agtttcgacc gctccgacaa
     1501 cgttgggcac caacaccggc tgcgggcctt ttgatacagt cagtttcacc gtggcaccgc
     1561 gtccgacatt ggcagcaccg gctaacgggt cctggccgaa gactgttccg gccgcttgcg
     1621 gcccaaagag ttcggtcacg accaagggct tgaggctggc ggcctcgatc gcggtacttg
     1681 catcgggcag cagttgcccg atcacgttcg gaacagcaac cgcagtgggg gagagatcga
     1741 tgagaaacga ctgctcggtg cgcgcacctt gtgtatcaac tgcggcaacg gttatcacaa
     1801 ttcgctgagt gccgcaggcc ctgcaactgt acagattcag gttgccggtg accgcatcaa
     1861 ccgtcggccc tgggtaggat gtcgcgtcga aactgctggt gaccaactca aaatgcaccg
     1921 tgtcaccgac gtccgggtcc acagcggtga tggtgtagat cgtcttcaca ccgtccgggc
     1981 tcgctctgag aagcgcgctg ggcggtatcg aagtgatcac aggcggtctg tttggcggct
     2041 tgatgtcaat ggccacgatt gccggtgccg aatccgccgc gccatcattg gccttgtacg
     2101 tgaatgctgt gctctgcgcg atacgcggat cgatcaccgt gccctgctga atctgctggc
     2161 ggaaatcgcg gcgcaccgag gcgtcgtaca aaatttgcgc ggtgtcgttg atggccgtgg
     2221 gacggaagtt gagctggttt tgcactttag gccccggcgc ccagttgttg tcacctccgc
     2281 tgatgcgcat cgcgttgccc ttgcagccac ccggcccaga gccccagtag tcacagttcc
     2341 atcgattggc aacatgcacg agaatgtcga cgtggccgtc gttgtcggca tcgaccagga
     2401 gtggattgcc gtgcaggtaa tagcgttcgc tttcggtatc gatgctccac agttcggcgc
     2461 cagtgcgccc gttgaggata aagaggcggc gcgtgctctg gaggatgact tcgggcacgc
     2521 catccagatt caggtcgtag acgtagagcg gcgaatcggt ggtgcgcggc atcgcgatgg
     2581 gcgcgatggc gggcacgaca tcgctgatga cgtcgggcgg cagcttggcc cagagcaagg
     2641 acccatcctg acgataggca tagacgaaac tgtcaccggc aagcagcacc tccggcgcac
     2701 catcgccgtc aacatcggcc acgctgagca ggccgttgat ccaggtaaac gggataggaa
     2761 tggtgcggcg cagagtgccg tcgtgattga agatatggat gccgcccgcg ttcttttgcg
     2821 ggccgttgtc gtaaccaatg atgtggatga tgacctcggc cttgccgtcg ccgtccagat
     2881 cagccaccgc gaccgaggtg ggctcaaacc cgagtttcac cccagaagtc acggaagcaa
     2941 attccgcctg ccaggcgagc gtccacaccc cacccgccag cttccagaca tcgccgcccg
     3001 agatgatttc gacctggccg tcgccgtcga ggtcggccac gatcggaacg ttttgcttgg
     3061 tcggcgtacc ttgagggacg tacgtgttca cttggttggc ggcattcggt gccgtgagca
     3121 cctggtcgat ggcgccggtg gcggcatcga tcacaaaggt cgccttgcag gctcggcctt
     3181 catccgcggg caggcccgtt accgtgcggc aggcggcgcg accaaagtgg ttggaattgg
     3241 gtgtgtcata gtaggggcca tagacatcgg tagccaaaaa gcgggtcaat aacttggtcg
     3301 atccgcccgc cgtgagtttt gccagcgtgg gcaccgatcc gtctgcggac gccccgaaga
     3361 atggcgtgat cggcgggtca gccggcgcta atgacgaggc ggtggcataa gcaccgggat
     3421 gcggccgcga caagcgctcg ctcttccact gcgttgccac tttgccgccg gtcagtattt
     3481 gcgaggcgtt gacggcgaaa aggcggtccg ggtagccggc gtagccgcct tggtcgcaat
     3541 tgatcgaaga aaaaaggtaa atgtcgccac caacgacatc gctaacattg cccagtgcga
     3601 actcgtccgt ggccgtgccg caagccgata cgtttgcgtg ggtggtgatc gaacggtccc
     3661 attgccacaa ctgcgagcca ttgctgccgc gcttggcacg gaaatcgccc aaggcggaca
     3721 aaacatactc aaccacgtcg tcgtggtcga catcggccgc tagtacgaaa tgatcgttgc
     3781 cgtctcccac tgcacgccag ctcacgaccg gattgagccc cggaacggcc ggcagcgtag
     3841 cgggcgcggt gtaattaaaa ccgccgtcgg cgttgaaagc gttgatcgtc cctttgtcgg
     3901 gatcactcag cttggcggct gtcagcgggt tgccgctcag cgacaggtcg ttgccgagta
     3961 cgccagccgc gggaatggtc agcgtttctc ccagacgaac ctcgtagaca tcgtctcgcg
     4021 ccagcggcgc gacgttgttg ccagtggtca gcgtgaacat ggcgacatcg accgcaccag
     4081 cgacatcgct aactttggcc ttgatgatgt ggtcgccacg ctgcgacggc gacggcgacc
     4141 aggcaagttc gcccgatgcc gaaatcgtca gcccggcggg cccgctgatc aacgcgtagg
     4201 tcagtgaatc gccaatgtcc ggatcaatcg ccaccagcgt gcgcgcgtaa gttgtgccga
     4261 cgctggctgc cgcgtttgcc ttgcttgcgg cggtgaacag tggcgtgcga ttggcgttct
     4321 gcacgatgac gctgaagctc gcctgtgcgg tgctgccaac ggcatcgcgc acctgcacag
     4381 tcaccagatg ggtgcccact tgtccggcgg agggcgtgaa cgtgaatctg gacgagccgg
     4441 tgggcaccag ggctgcacca gtgggggcca caagcaggct gtaggtcagc gtgtcccgtg
     4501 ggttgatgtc gttgccggga agggcgacag acagcgtgtc cccgagcctc accgtctgcg
     4561 tcggaagtgg gctaaatgcc ggcgcggcca ggggttgcgc cagcacggtg acggcaatgt
     4621 cgtgactggt ctccaactcg ccatcgctgg cgacgagccg cagcacgtag gcgccgggtc
     4681 cgaccggatc gaagctcacg gttgttctgg cggtggtcgt cgagttaaac ccgaccggag
     4741 caaacgcggt cgacggtgct ggcggcaaca ccgcaggacc cgaaacctgg gtccagcggt
     4801 aggtcagccc gcccggcggg ttgggtaagc catcgtcggt caccgccggg tcgagatcaa
     4861 gtgactgcgg caagacgacc gtcactgcgg cctgggtgat cgacggtgcc tgattaaggc
     4921 tcacccccgg tgtcacagta atcgcgactt cgccgactcc tgtctgcacg ctgtcgctgg
     4981 cgctcaggcg cagcacatag gcgcccgcca aactgaaact cgcggaggtc gcagcactgg
     5041 cagcattgcc aaaagtaaca gtccccggtc cactaacctt gctccaggca taggtcaggg
     5101 tggctggcgg attgggtagg ccgtcatcgg tggcactgcc ggaaagcgtt gcaaccgccg
     5161 ccagtgcgat ggtctgattg ccaccggcat tgacctccgg ctgctggttg cccgcctcgc
     5221 gtgccagcac catgtcaacg cgcaatgcca gattggcgcc atcctggatg gcgtagagcg
     5281 caaccagatc ctggcctgct ggcgcatcga ccgccgaatc agttgccagc ggcgcaatgc
     5341 cggtccagtc gacaccgttg ccatccagca caatcgccaa ggccgagacc gtgctcaaag
     5401 tgcccgctac caaacacgac agcagcaaca gcttgccacc ctcgcgtgca tggcggcgca
     5461 tcagccagcc agtgctaccg atcagcgata acagaatcag catgccggtt ggcgacaggc
     5521 ttgggattgg ctcgggtgcg gtcggtgcca atgggctcac agcaaacacc ccggccgcga
     5581 tcaaactgtc gcttgatggg gtgatggcgc ccatctttac cgacgaggta cctcccagat
     5641 cagccagcgg gataaaggtt tcaatcaggt cggacccgtt gagcccggcc gacataccga
     5701 ccggccagcc cccggcactg acgttgatgg gggcaccgaa cacacctgca atgcagatgc
     5761 tgcgtgtgat cgttgagact gtaccggtat tgaccgccgt cgtcaccacc gtgtagagca
     5821 cagtttcaac gccgggcatt gaactgctgc cgctggtcag tgtgcaaccg gtagcggcgt
     5881 tgttgtccgt gtcgaagccg atagccaccg ttgttgtagc agcgtgcgcg cctgctgctg
     5941 agatggtcag cagcaaaccg acaaacccca tggcaagcgc atgccgcaat ttccgagtaa
     6001 aagaaatggc tggcatgggg atatcctttg aaagtatggg ggcggctacc aagacaaagg
     6061 ctgacacttc agagccttgc gcgacaacac gtcacattcg actcaataga cttttttgag
     6121 gcaacctggc aggaaaacaa agctcgtacc ttgaatccag ccattgaaca atgaccaacg
     6181 atgaataatc tcgggtccac tgtattcgat gccagccttg cgacgaggta tcaggccttg
     6241 aaacagatat caatcgacac gaaagccctt gatctgttgg caaaacccca agtttcacca
     6301 ccttgaggtg aactgctcga ttgggccttc acttacccaa cggccaattt aacgcacagg
     6361 catcgaaagt cactgtttgc gcttcaagcc tttgcccgtc gctctcggcc aaaacccaaa
     6421 tgattcctga tgtcgcaggc ctgacccaag caacccgctg acaaccgact tgcgcccgcg
     6481 acgtgcaact caattgctat taaataaata gctgttagcg cccattccac gggggctaca
     6541 gccatatttc tcatgtttta tcgggctgcg atgtagtcgc gaggcggaag ccaaagctca
     6601 ggccagcatt tccagcaaga ctgcgcgcca gacctctttg tcgattttgc ccatgttcat
     6661 gcctgtgctg gccctgtcaa cacagcgcaa ttgttcaagc aaggcgcagg atttcttgcg
     6721 ctgaagaacc atttcgggtg acaacccagg ggttccccgg cgctggcgta gggggcgaca
     6781 atgaccctgg gcaaggtggc cttgatgtca agcggcgagc cagccagcgc ggggcaggct
     6841 tttgaatttc tgagccaacg gtggattcga gattgacccc atgcccttcg ccgcttgcca
     6901 taacgcggtt ccccataccc actcgctgtc gtccgaagcg gccgattcaa cccaaacctg
     6961 tgcctgcggc gcgccgtgga ccaaggcagc ttctgatgcc gcctgccagc atccaacaaa
     7021 aggaatcgcc aggcagacga caggagaaac cgggcaaaca acacaataaa gcccgacctc
     7081 ccctaattgg caatgcaagc gacttgacaa tgcaagcgca gttgctgctg atgaatcacc
     7141 aggtgaattc ctgatatctg gcttattgaa ggatcaaatc cggctccagc cctttatcag
     7201 tctgcgttga ctgctacatt taatgaaaat tttctgccct caataggcaa tcaacatcca
     7261 tggcaatgac acccccacgc aaacttatat ttgacaccga ccctggcgtg gacgacgcga
     7321 tggcgctgtg ttacgcgctg gcgcacccag gcctggatgt gatcggcatc accaccacct
     7381 ttggcaatgt cacggtcgag caggccagcg ccaatgccct gtacctgtgc gcgctggccg
     7441 ggcgcagcat tgcggtcgcc caaggcgttg cacggccaat ggtgaaacag gcagagtcac
     7501 cgcccgactt tatccacggt gccgacggcc tgggcaatct gcccgagcgg caccactgca
     7561 gcgcgcaggt cgattcgcgc agcgccgcgc aattcatcgt tgacacggcc cggcaaagcc
     7621 cgggtgacat cactttggtg gcggtaggcc cactgggcaa tctggctgct gcgctgcaac
     7681 ttgacccgga actgcccaaa ctggtgcgag aagtggtgct gatgggcggt acggtgatcg
     7741 agtcgggcaa cgtgtcgccg gtggccgagg ccaatatctg gaatgacccg cacgccgccg
     7801 acatcgtgtt cacggctggc tgggaactca ccatggtcgg actcgatgtc acccaccagg
     7861 tgattctgcc agtggcactg ttcgagcaaa tcgcagcgca ccactgccac gctgccaccg
     7921 acgcactggc gcacgcggtg cggttttaca gcgcgtttta cagccagcac gacccgttgg
     7981 cagggcaaat caacggttgc tatggccatg atgtgctggc atttgtggtg ctgaccgacc
     8041 ccgacctgtt cacgctgcag cgcgggcggg tgcgggtggc agtggatggg ctggcgcaag
     8101 gccagaccat gatgcaacgc aaggcggtga gctacccgca gcacggttgg catgacgaca
     8161 ttccgcacac ccaggtgtgc ctgcaagtgc aggcacaagc ctgccgcgag gtgattgagc
     8221 aaacgctgat gcgccgctgg ctgggctgat gcccttgcag tgctggtgcc gcagcgacgg
     8281 gcaaaggccg caagactgag ccaaacctca gccccgctgc cgcagcatcc accgcatcat
     8341 cacaccgtgt caggcacgct gggcaagccc gccagggcca tggccagttc gctttcgtcg
     8401 tatttctcgt cggtcagttc gccggcaaaa tacttctggt aggccgccat gtcaaagtgc
     8461 ccgtggccac acaggttgaa caaaatggtt tctgacttgc cctcacgctt gcaacgcagc
     8521 gcttcaacga tggcacccct gacggcatga ttggcctcgg gggccggcac gatgccttcg
     8581 gcacgggcaa acatcacgcc agcttcaaaa cactcgacct ggttgtacga caccgcttca
     8641 agcagcccga gctgcttgca gtgcgacacc atcggcgcca tgccgtggta gcgcaggcca
     8701 ccggcatgaa agccaggtgg cgtgaactga ctgcccaagg tgtgcatctt ggtcagcggt
     8761 gtcaggtggc cggtgtcgcc aaagtcatag gcgtacttgc cacgggtcag cgacgggcag
     8821 gcggctggct cgaccgccac catgcgcggc ttggggccac cgcgcaggcc atgaccgatg
     8881 aacggaaacg caattccggc aaagttggag ccaccaccgg tgcagccaac aatcacatcc
     8941 ggccaggtat cggccatttc catctgctgc atcgcttctt ggccaatgat ggtctggtgc
     9001 agcagcacgt ggttgagcac cgagcccaac gcgtatttgg tgtcttcgcg ctgcaccgcg
     9061 agttcaaccg cttcagaaat cgcaatcccc aggctgccag ggtggtcggg gcgcttggcc
     9121 agcaccgagc ggccatagac cgtctcgttg gatggcgaag caacgcagcg cgcgccatag
     9181 gtttccatca ccgcacggcg atagggcttc tggtcatacg acacacgtac ctggaacact
     9241 tcgacttcca tgtcgtagag cgagccggca aaggccagcg aggtgcccca ctggccggca
     9301 ccggtttcgg ttaccagccg cttgacacca gcctctttgt tgaaaaacgc ctgcggcacg
     9361 gcggtgttgg gcttgtggct gcctgccgga ctgacccctt cgtatttgta aaaaatcttg
     9421 gcaggggtgc caaggatttt ttcaagccgg tgcgcacgaa acatgggggc gggccgccac
     9481 aggcggtaaa tgtcacgcac tggctcgggg atttcgattt cgcgctcaag gctgacctct
     9541 tgcatgatca gcgacatcgg gaacagcggc gccaggtcgt cggggccaac cggctgcatg
     9601 gtgccagggt gcagcaccgt tggcaagggc ttgggaaagt cggcttgcag gttgtaccag
     9661 gccttgggca tctggtcttc attcaacagg aatttggttt gtttgctcat cgaaaaagtc
     9721 ctcgttacag aaaaatgctg gtcattgaac ggtccagtat gcccctgaac ccaacacccg
     9781 ccaggcgtgc aaattcaggg tttaccctaa aacaccagcg tcttgacacc gctggcggtg
     9841 cccagcaggc agacctgtgc cttctggtgt gcaaatacgc cgacggtcac cacaccgggc
     9901 cactggctga ccagggattc gaacgccagc gggtcggcaa tctgcaggcc agtcacgtcc
     9961 aaaatgtgtt gtccgttgtc agtcaccagg ggtttgccgt ctttcaaacg cagcacagcc
    10021 cgcgctgcca tggcctcaaa ctgccgcttg atgcgctgcg ccgccatcgg aatcacctcc
    10081 accggcaacg gaaaccggcc cagcgtctgc accagcttgg agtcatcagc gatgcagaca
    10141 aatatgcgcg actgggcggc cacaattttc tcgcgggtta acgcggcacc gccgcctttg
    10201 agcatgttgc cgtggtgatc aatctcgtcc gcgccatcaa tgtacaccga cagttctgcc
    10261 acctcattgc agtcaaacac cgcaatgccc aacgcccgca ggcgctcggt gctggcctcg
    10321 gaactcgaca ccgcgccctt gatctggtct ttcatgctgg ccagggcgtc gatgaatttg
    10381 ttgacggtgg agccagtgcc gacgccgaca acttcgcccg ctgccacata gtgcaatgca
    10441 gcctggccca ccagggtttt gaggtcgtcc tgggacaaac tggcggctgc agcggtttgc
    10501 acttgtttca tctgggaaaa tccgttgggt tgatcgattt gatttcagga attatccaat
    10561 ggcacttgtc ccctacccgc tggcacgacc gtttttattt ggcctggacc ccgaagtggc
    10621 acacgagctg acccttggcg cgctggcgcg cacccagggc tcggtgctgc aattggccta
    10681 cgccagcgtc cgcgttgatg accccaggga ggtggccggc ctgatcttcc caaaccgggt
    10741 cggcctggcc gccgggctgg acaagaacgc ccgctgcatc gacggcttgg gggccatggg
    10801 ttttggcttt gtcgaggtcg gcaccgtcac gccactggcg caaggcggca acccgaagcc
    10861 gcgcatgttt cgcctgccgc aagcgcgcgc gctgatcaac cggctgggtt tcaataacga
    10921 ggggctggag gccttcgttg ccaatgtcaa gcagtccgct ctttacctgc gccgtcaagg
    10981 cggtgatgac gaatccgacc cgatgttgct gggcctgaac atcggcaaaa acgctgccac
    11041 cccaatcgaa cgtgcgaccg acgactacct gacctgcctg gacggcgtgt acccctatgc
    11101 cgactacgtc accgtcaaca tctccagccc caacacaaag aacctgcgca gtctgcaaag
    11161 cgacaaggcg ctggatgcct tgctgggagc ggtgtcggag cgacgcgaat tgctggcaca
    11221 gcggcacggc aaacacatcc cggtctttct gaaaatcgca cccgacctgg atgccgacca
    11281 actcgaggtg attgcccgca ccctgcaacg ctatggcacc gaccggactg gccgggcaaa
    11341 caacgcctgg ggagtgatcg ccaccaacac cacgctgagc cgcgaggcag tggttggcat
    11401 ggcacacgcc caagagaccg gtggcctctc aggtgcaccg gtgctggcca tgagcaacca
    11461 ggtgatccgc cagttgcgcg ccgcactggg caaggatttc cccatcatcg gtgccggtgg
    11521 catcatgagc gccgcggatg cggtcagcaa aatagcggcc ggggctgatg tggtgcagct
    11581 ttacaccggg ctgatttacc agggtccagg gctggtaagg aaagctgctc tgatgatcaa
    11641 aaacaccttc tagcccttat gcctgttgcg ctgccagcta cttattttgt agtgacaagg
    11701 atcttcacca cagcttgccc aatggccagc gcactggtca ggccgggcga ctcgatgcca
    11761 aacaggttga ccaagcccgg caccccgtgg tcctggggcc cctgaatcag gaaatcagcc
    11821 gcagcagcat ttggaccact gatcttgggt cgaatgccag cgtaaccggg caacagtgca
    11881 ccgtccggca gcgccggcca gtacttgcgc acctcggcat aaaacgcatc acccctggcc
    11941 gggtccaccg tcaggtcgtc tggcgcagcg acccactgca cgtctggccc aaatttggct
    12001 tggccaccga ggtccagtgt cagatgcaca cccagacccg cagcttctgg cagcgggtaa
    12061 atcaaatgcg aaaaaggggc ccgtccggcc agcgtgaagt aattcccctt ggcataaaaa
    12121 gcctgcggta caagctgcgc tggcacgccc ttgaaacgtc tggcgacatc gggtgcttgc
    12181 aaaccggcgg cgttgaccag cagccgaacc cttaaccggg tgccgtcaga tgtcctcaat
    12241 ttaatagcac cttgcgcata ttccataagg gctagaggcg aattcagtgc gacaacaccg
    12301 ccagcactct ccagctcacc ctgcaggctg agcatcaatc catggctatc gacaatgccg
    12361 gtgccgggcg acagcaaggc cccctggcag cgcaactgcg gctccatcgt caaagcttgc
    12421 tgctgactta gcagcaccag gtcatccacc ccgttgcgcc tggcttggtc cgcaatcagc
    12481 ttgagttgac ccacttgagc gactgaggtg gccacaatca gcttgccgca gcgctggtgg
    12541 gcaatgtggc gggcagcgca atagtcgtac agcatcgccc ggccctgcac acacaagcgg
    12601 gctttgagtg agtcttgcgg gtaacaaatg ccagcgtgaa tgacttcgct gttgcgcgaa
    12661 ctggtgccgg tgccaatggc gttcgcagcc tccagcgcca gcacctcgcg cccgctttgt
    12721 gccagggcac gcgccaccgc caaacccacc acgcctgcgc ccacaacgac gcaatcgact
    12781 tgctccatgg cagctaggtc aggtgaccgc gaaaatcacc tgcgcgcatc gggcttaggt
    12841 tggtgccaag gcgcaaaccc ccgcgggcga tcaaaggttg gccgagcgcc accagttgct
    12901 gtgatctttc ctgagcaagg cctgcgaagg catcgggtaa tgcacgaatt cggaatccag
    12961 cccgacgtag aagccaccct tgaccatgcg aaacggaaat accagtgaat gcacccggtg
    13021 caccagcttg ttgcgcttgc cgcccggctt gagttccatc aaggcttcgc gcaagtgctg
    13081 catgaacgaa taaggcaagt cgccttcctg gccattttcc ggatcacggg cgagcgggcc
    13141 gagttcgagt tcaacaaaac acagtcccgg aaaacggaaa aacttcgatg gtgtggtggt
    13201 cacgtcggag cagaaaaagg ccgggtccag acggctcacc accaggctat tgatgggtgc
    13261 caggtcctgg tacatgtgga gttctggcac cggctgcgga acttcactgg cgcggctcaa
    13321 gcccaaggtc gcgccattcg ccgttgacag gtacaacttg cccagcgatg ccatggtgag
    13381 gtgctccatc acccggtaca tcgagatata gaccgagttc tttggcgagc catccggctt
    13441 tgccacgcat tttgcaaatg cgtcatcaat gtcgaagtag gcatggcggt aagacgggtc
    13501 gatctcgaaa aaaaccgcct gcccctttga cttgaacttg tggccggttg catagtattg
    13561 cccgaattcc tcgggcggca acatggaggc aatcagcgct tccggaatca gcgaaaagta
    13621 aagatgaata gccatttgaa cccctctttt aaaaatcaac ttggaaatca acagttgtca
    13681 ttttcaccat gacgaccggc ataagaaagc gctttatgcg gggtgacggc gttgagaccc
    13741 agcaaacttg ccagggctgt tatgcagcag tctccttgga gtcgatccgc atgccgtaaa
    13801 aactgcggaa cacaaaaacc aggtttaccg ccagaaatat cgccgccagg ccatagacca
    13861 ctgattgatg accctgggca ttgagcccga cagccatttc aaccgccagc agaccaaaca
    13921 gcgtggtgaa cttgatgatt ggattcattg ccaccgaagc agtgtccttg aacgggtcgc
    13981 caacggtgtc tccgaccaca gaggcattgt gcagatcggt tcccttggca tgcaattcgg
    14041 tttcaacgat cttcttggcg ttgtcccagg caccgccggc gttggccatg aataccgcct
    14101 ggtacagacc aatgatcgcc agcgaaatca ggtagccgat gaagaaaaac ggctcaatga
    14161 aggcgcacgc cagggtgccg aaaaacaccg ccatgaagat gttgatggtg cctttttgcg
    14221 cgtaaatggt gcagatttcg accaccttct tgctgtcttt catggatgcc ttgacgactg
    14281 cgtccagacg aatgtgcgtg ctgatgaact ctactgcacg gtaggcaccg gtggacaccg
    14341 cctgcgtact ggcacccgag aaccagaaaa tcgtggcacc gccagacacc aggccgagca
    14401 ggaacggcgg atgcagcagc gacagcttgt cgaggttgtc cgtcagcccc aaagtcagca
    14461 gcatgacgat cgaaaacacc atggtggtcg ccccgaccac ggcggtgcca atcagcaccg
    14521 gtttggcggt ggccttgaag gtgttgccgc agccgtcgtt ttcttcgagc aaaaacttgg
    14581 cagcccgaaa atccagattg aagccgaaat cgcgcttgac ctcagcctcg atgcctggct
    14641 cctgctcgat cagcgaaagc tcataaactg actgcgcgtt gtcggtaacc ggtccataac
    14701 tgtcaacggc gatcgttacc gggcccatgc caaggaagcc gaaggcaacc aggccgaagg
    14761 agaacaccgc agccatcatg acggccttgg tatggtccgg gttgatcacc gcagtcaggt
    14821 cgaactgcga cacgatgtag gcaccggaca tcaaaccgac gatgatcacg ccaatccaga
    14881 aggctgaaaa atagccggcc acgatgcccg acaaaatgtt gaggctggca ccaccggctt
    14941 tggaagcggt caccacttcg cgcacgtgtt tcgagtgggt tgaagtgaag accttgacca
    15001 cttccggaat aatggcaccc gccaaagtgc caagggtgat gatcaatgaa agctgccacc
    15061 aaaggttggc gtaagccttg cccgccacgg caaagtctgc catcagcagg taagacacaa
    15121 tgaaggtcaa aatcagcgag acaaccgagg tgatccagac cagcgaggtc agcggtgctt
    15181 caaaattgaa gcgcggtttg tccccgtaca aagcccgcgc cagcaggtcg ttgccaaaat
    15241 acgaaacgcc cgaagccaca atcatcatca cccgcatgac aaacagccac accagcagtt
    15301 gcacctgcac ggcgggctca gggatggcca gcatgatgaa ggtgatcagc gcaaccccgg
    15361 tcacaccgta agtctcaaaa ccgtctgccg tcgggccgac cgagtcaccc gcgttgtcac
    15421 ccacgcaatc ggcaatgaca ccgggattgc gggcatcgtc ctcttgaatg ccgaactcga
    15481 tcttcatcaa atcggagcca atgtcagcga ttttggtaaa gatgccaccg gcaatgcgca
    15541 gcgcagcagc acccagcgac tctccaatgg caaagccgat aaaacacttg ccagccattg
    15601 ctggcgagac aaacagcaga atgcacagca tgatcagcag ttcggtcgaa atcagcagca
    15661 tgccgatcga gatacccgac ttcatcggga tcgcgtaaac cggaaaaggc ttggccgcca
    15721 gcgaggcaaa tgcggtgcgg ctattggcca gggtgttgat gcggatgcca aaccaggcga
    15781 cggcaaatga accggcaatg ccgatcaggg aaaacgccag gatcagcgcc acgtcggaca
    15841 ccgccagatg ctggacgaag taaaaatagc caatcatcag cacggcggta caggcctgca
    15901 ggatcagcag gaacttgccc tgcgtgaaca tgtaggtctt gcatgtctcg tagatgagtt
    15961 cactcacctc ggccatggtg cgatgcactg gaaggtcgcg aatctggcgg tagatgacgg
    16021 ccccgaaaat cagcccgccc agacacacca acagcccgta tgaaagcagt gtggaacccg
    16081 acatgccgcc aagaaacgaa atcagcgcgg tatctcccaa gttgggcaaa accagatcta
    16141 cctcgctggt gtggccgctg gcagccaagg cagagcctgc cccaatactc agtagcaata
    16201 acatcagcaa ccggcgcgca accccaaacc attgggattc aattgaagag tttccaaatt
    16261 tcatcaatgt tccttacaag ataacgaggt gaaatcgcgc cgcaagcctc atcagactgt
    16321 cttgtcgagc gaagacgatg cgggctcgac ctgccaatga actatagctt gtccaaggga
    16381 cacagccagc ccagtgccgc ccgaattcac gcgtgttagt gagttaagtt tgttttcatt
    16441 ggtctgccag cgttatgccc agccagactg gcttgtatga gacacttctg tggccagatg
    16501 gggcctgtcg gtagctcaag tcatccttga cccccgccct ggcctggtta cacgcaacat
    16561 tggtacccga accctcacaa aaatcatgtc ccgcatactg atccgacttc cagaaacgtt
    16621 cccgttttgt accgacatcg tgctttacca aagccacatc aattacggag gccatctgga
    16681 caacgcactg ttgttgaccc tggtgtcgga agcacgagtg cggtttttca agtcattggg
    16741 ctataccgaa cttgatgtgg agggtgtcgg catcattgtt tcggatgctg cgctgcaata
    16801 ccgatcggaa gcctttcatg gcgaagtgat gctggtacgg atagggccag ccaacttcag
    16861 tcgcaagggt tgcgatcttc tgtggtgcat gaatgagcaa tcgagcgggc gtgaagtggc
    16921 gcgcggtaaa accggcatcg tcttctttga ttacgcaacg cacaagactg tgccggtgcc
    16981 acaagccttt cgcgacaggc accccgccga cgagccattt caatgagtca ccttgttgtc
    17041 aggtgacaac attggaaatc agtcgcagcc ccgtgttccc ggtggcgcgg attctcaaaa
    17101 cgtcggggtt ccgacacatg attcacctat gcccgaccct atccgcaaca ccaccaacca
    17161 ggcatttgca aaatcaacat accgcgccaa gctgacgctg cgttggtagg accgcgtcca
    17221 acgggcagat caagccttca ccaagcgtgc ccaatttgtc gaggaaaatc caaaactgcc
    17281 tttgaatctt gctgcgttga agaacagcca agcagcgggc aatagtgcta tctggttagc
    17341 agtcaatcca aatggctgca tgggtgatcc agatccgccg ccaggtcaat cgccgatttt
    17401 ctgccacatt gccaatggaa ttcacggcga cgcaccggtc tgctcacagg tactgccggt
    17461 gccccgatgg cccaggagcc agtcggacat ctatagcgaa aatcgacccc agcgaggcca
    17521 gtttgtgccg gatttgccag cccacagtcc aattgtgggt caaatggccg gtgcaaaatg
    17581 agccgctcaa gccggtttgc agccgatgcg ccataagtcc aacaagctcg ttgtgctttg
    17641 gttggcatct gtgacgagcg gccgctcaac tttgccacca ggccagcgca acaggaaatt
    17701 gcactgagtc cgcttactga ttgtttttac aaaaccccct gcctgcgtca ggctgctctt
    17761 tgaaggtact tcagcacgcc ttgaccggcg cggacaccgg tggccaagca agctgtcatc
    17821 aagtaacccc ctgttggtgc ttcccagtcg agcatttctc cggcacaaaa gacaccgggc
    17881 atagccgtca gcatcaggtt gtcgtccatg gcttcaaaga caacgccgcc ggcactgctg
    17941 atggcctcat caagaggtcg cgttgcactc aaggtgacag gaatcgcctt gatggccgcc
    18001 gcaagtcgaa ccggatcgtg caactgctct ttcgacagca gttcatgcag gatcgccatc
    18061 ttgatgccgc tgatgtggag ccggctcttc aaatgactcg acagcgagcg cgaaccacgc
    18121 ggatgacaca cctcggccaa cacacgctgc acagacagat ccggcaagag gtccagataa
    18181 aaagtggctt gaccctgcac caggatatcg tcgcgcaaca acttcgatgc cgcgtaaatc
    18241 aggcttccct ccaccccggt ggcggtcacg acaaactcgc ctttgcggtg aaagggctcg
    18301 cccagatgac tggtcatggc aatggccacg gacttgaatg gcaggccggc aaatctgttg
    18361 gcaaagtgat cggtccagcc acccaaacgg tcaaaaccac agttggcagg caacagtggc
    18421 gaaaccgcaa caccagagga acgcagccat ggcacccacg ccccgtcaga cccaagccgt
    18481 gcccaactgc caccgcccag cgcaagtacc acggcatcgg catcgacctg tgtcggccct
    18541 gtcggcgtgt cgaagtcaag gcgggttggc gcaccagcag gcgagtgcca gcccgtccag
    18601 cggtttcgca tgtgaaactg cacggcgtgc ccggcctgcg gatgccgcag gcggtgcagc
    18661 caggcccgca gcagcggtgc ggccttcatg tctgctggaa acacccggcc cgaacttccg
    18721 acacaggtct ctatgccgag cgagcaagcc caggaacgta catcctgagg gtgaaagccc
    18781 tgaaaagtgg tctccatctg ctcgcggcgc gccccgtagc gcgaaacaaa cacattttca
    18841 ggctcggagt gagtcaggtt aagtccccct ttacctgcga gcaagaactt acgcccgacg
    18901 ctgggcatgg catcatgaac gtcaaccacc attcctgcct tggcaaggac ctcggcagcc
    18961 atcaaacctg cagggccagc acccacaaca attatttttt tcatcatcct atattcagtt
    19021 gaacgcgccc gagtgggccg ttggcagcgc ggctgggtag gcacgacgac gacgcacagc
    19081 gactgcccgc atgtcgtcct ggcagacgcg gacggcgtaa cgaccccagg cgggttacca
    19141 gtggctcaat cgggtgcgta gcgcttctac ccaaaaggcg aaccccatcg aagctgaaaa
    19201 agtcaaaatt catgaggact ttcagatgaa aacaagctgt caactgaggg ttcaaggatc
    19261 atgcattcaa tcatctggtc aagctggcaa gcgattcagc cagaccacat tagtcctttt
    19321 cgactaatgc agcaacagcg tgttgcgcgt cagcgctgtt ctacaaacca gtttctgtca
    19381 caatttcggt attagacgct attcaacaaa ccaaacagga tcaaaaatgg ccagtgaact
    19441 gattaaacat gtgtccgatg cttctttcga gggcgatgtc ctcaagtccg agttgccagt
    19501 actggtcgac tattgggctg aatggtgtgg tccctgcaaa atgattgcac cgattcttga
    19561 cgaagtatct gcagactacc aaggcaaact gcaggttgcc aagatgaatg tggacgacaa
    19621 ccgcgatgtt ccggcgaagt ttggcattcg aggcattcca acattgatgc tgttcaagaa
    19681 tggccagttg gccgccacca aggtaggtgc catgagcaag tcgcaactga tcactttcat
    19741 tgaccagcaa ctggcttgat cgattctttg gccaggttag cttgggcgtt cgcacataac
    19801 gccccatcag ggagttttac tgtcataatc ccgctccagc accagctcgc catcagggaa
    19861 tagccagagt cgtttggcag cggctcatgc gacttcattc atgcaagtcc ggccctcaca
    19921 tcctccccgc tttccagttt ttttatccac cgcttccatc catgcactta aacgaactca
    19981 aggcactgca cgtgtcagaa gtcctcaagc aagccgaaga tcttgagatc gaaaacaccg
    20041 ggcgtatgcg caaacaggaa ttgatgtttg ccatcatcaa aaagcgcgca cgtgcgggtg
    20101 aacagatttt tgctgatggt gtgcttgaga tactgcccga tggttttggc tttttgcgta
    20161 gtcccgacac cagttacacc gcctcaactg acgacattta catcagccca agtcaggtgc
    20221 ggcgctttaa cctgcacacc ggtgacatga tcgaaggcga agtgcgtacc ccgaaagatg
    20281 gtgagcgcta ctttgcgctt aacaagctgg aaaaagtcaa tggcggcggc ccggaagaaa
    20341 acaagcacaa ggtcatgttc gaaaacctga caccgctgtt ccccaaggag cagatgcggc
    20401 ttgagcgtga aatcaagggg gaagagaata tcactggccg catcatcgac atcatctcgc
    20461 cgattggcaa gggccagcgc gcactgctgg tagcaccgcc caaga
//
LOCUS       DAOSLL010000007        20092 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_2921_length_20092_cov_4.728602, whole genome shotgun sequence.
ACCESSION   DAOSLL010000007 DAOSLL010000000
VERSION     DAOSLL010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 20092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20092
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2921_length_20092_cov_4.728602"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="PLB25_00575"
     CDS             <1..1037
                     /locus_tag="PLB25_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40122.1"
                     /translation="IAVTTSTFTYDITGTPVSPATGASITSKVAPLGWEIAFTATNKR
                     AYRSKNVLSNRPYLRVDDGCDPAYTTTYAKKAKVTMAQGMSDIDTFVGARAPFDSAYP
                     TRNEIATGSGAGVYDGWYKWYYARSDGNAYDHYGASVFNRPWTLVGDDRGFYIFNDTW
                     SSGGLGGKCFTDFESYRSADGFNTLLCAQEAYNYASIPSSSYDSEGYQSSDWRSRFPR
                     TLDGTGKILMRSYLQIGNNVNPSFTSLNTNNGQTTSGYSTGISWPNGPDYSMILHPTL
                     LRESSHLRGRMPGMFWVHNDSPSFNHLDTISGVAGYPGRTFLLLKVAHGVSSGNYTAT
                     LAYDITGPWW"
     gene            1041..2135
                     /locus_tag="PLB25_00580"
     CDS             1041..2135
                     /locus_tag="PLB25_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40123.1"
                     /translation="MSWWISAAGLTPIIAYDSSHFQSNAVLTDLTGGGNHADMAGQSA
                     SLVVVGTNPIQSITCVKGVGAGWSYQRVTRPTAGVLMALVSHVNPRVMLFSDYGVSGN
                     YHALLIETDVTTLSPHGQIRCEGSANSVGLPTGAAVKFVAIAFDASGYQYYCNGSWVG
                     AKFSVPLSLLSSVGSNWPSSWGMYANLSALGMFDGVASLADLQDLEAQVRLAVVGPPV
                     VFHGYGTDLSRVNTIPTVNLATQGLGDNTGRINTAPMQALDGLGVRNKALVPILGQHD
                     IYFGGVGQVVGTVKNSPATPVRRRVLLIEEATRAVIRETWSEAATGAYSFGRVAMNTT
                     YTVVSYDHTQAFRAVVADRVVPEVMLETVP"
     gene            2132..2539
                     /locus_tag="PLB25_00585"
     CDS             2132..2539
                     /locus_tag="PLB25_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40124.1"
                     /translation="MTLAISPAHKLARLTGTLSFADAGAQNSRIRLYATTQPSTGGDP
                     GGGLLVEILLAKPCGEINASVLALKQSEPSGDLILETGTALWARWINGRDEVVADGSV
                     SDAAGTGDFKLSGTSGTVLYAGARALLGQCTLT"
     gene            2568..4532
                     /locus_tag="PLB25_00590"
     CDS             2568..4532
                     /locus_tag="PLB25_00590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40125.1"
                     /translation="MPENLVFAGTPLIEQPLMMVFGEGVTDPNAKITDLVFAQPPRVH
                     PTQLVFGDSDTSTHPDSLLEVSGALPELVTQLRLVIGVPVAWQAALPDLVGTVAVKYQ
                     SQTQRPMVAQVQTWSQVSLVNESGLTQPQQHAQATNAGAQEQAQNAASIRQGVSPSYV
                     EAIRSSREVGSQFQDGCATRSRLHANWSDGLADRRVQSTGPFQEGLRAPTVRAVGRFE
                     DGLHDRREGIENSWAGAIPRALGYSGHAGAAIPLRRFLTSAFQDAWVPRPGVHLIPVT
                     PPVDPTYWGTALLFACPPLAAPMLVFGARQCEFPEEPPIGVVTIAVRKVYFVINDVNL
                     RRASNGVNVPVNSLSLSLDASSWAWGFDAVLPHSARSLIEPNGSGVVELLANVNGTTF
                     AVLAENISRERSFGQTSIRLTGRGKNAVLAAPYAPVMTFTNTQARTARQLMDDVLTIN
                     GVPMGWNVDWGLTDWNVPAGVFSHQGTWIEAMSVIAGAAGGYLLPHPRDQTIRVRHQY
                     PVPPWEWSTATPNFVLPVDAVEKESLRWLEKPSYNRVFVSGQEAGVLAQVTRSGTAGE
                     QLAPMVVDALITEAAVARQCGLAVLSNTGRQIEVSLNLPVLLETGIIEPGALVQYRDG
                     GIDRIGLVRSTQVQAGFPEVWQTLGVQTYA"
     assembly_gap    4745..4844
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5001..5294
                     /locus_tag="PLB25_00595"
     CDS             5001..5294
                     /locus_tag="PLB25_00595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003270772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6127 family protein"
                     /protein_id="HOE40126.1"
                     /translation="MEDLPIERRKTVTMPQADFEQMLERAAERGARHALHGVGLDGED
                     AAHDIRELRNLLDAFNEAKKTAGLTIIKMIVTGLVMAILTGTLIKLKLFGGGQ"
     gene            5291..5767
                     /locus_tag="PLB25_00600"
     CDS             5291..5767
                     /locus_tag="PLB25_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40127.1"
                     /translation="MIETLLGGLLGGAFRLAPELLKWLDRKGERGHELAMQDKALEFE
                     KIRGASRMGEIGASADAAWNSGAMEAFKEAVSGQGRPSGVKWVDALSSTVRPMVTYLF
                     VLMYAGVKLSTFVGSVQTGMGFGDALLAAWTEADQALLAGILNYWFLNRTIEKGLR"
     gene            5818..6261
                     /locus_tag="PLB25_00605"
     CDS             5818..6261
                     /locus_tag="PLB25_00605"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HOE40128.1"
                     /translation="MAERFWEKVDVRGADECWPWLASKKQGGYGRISDERGHPQLSHR
                     VSYALTVSPIPDGNVVCHRCDNPRCVNPAHLFLGTQADNLRDMRSKGRGNPPRGVEHP
                     KARLTEALAQQIRRDSRSHRQLARAFGIGKSTVGMIKAGDTWTLV"
     gene            6246..6716
                     /locus_tag="PLB25_00610"
     CDS             6246..6716
                     /locus_tag="PLB25_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HOE40129.1"
                     /translation="MDTRVIGVPPQAIELAKRFEGFHRVPKNDPLGQAHPYICPAGFW
                     TVGYGHLCDPKHPPITEAEAEVYLAQDLMTALTATLRYCPVLATESESRLAAIVDFTF
                     NLGAGRLQTSTLRRRINQLDWASAGQELRRWVYGGGKVLPGLVKRREAEALRLI"
     gene            7074..7412
                     /locus_tag="PLB25_00615"
     CDS             7074..7412
                     /locus_tag="PLB25_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HOE40130.1"
                     /translation="MGFRHLCEPDHPLITETEAEVYLTQDLVSALAATLRYFPVLATD
                     SNERLVAIADFIFTFGAGLLQTSALRRRINQLNWSEIAQKFRRWVHSGGKVLPGLVSR
                     REAELRLLDY"
     gene            complement(7409..8713)
                     /locus_tag="PLB25_00620"
     CDS             complement(7409..8713)
                     /locus_tag="PLB25_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40131.1"
                     /translation="MLDEYSIEQRATQIFDSRTKEYFAEVLSCYAAGNYRSSVVMLWS
                     VAVCDLLFKLQNLVDLYGDTKAKDILAEIGKLQQDNERSPIWETKLVESVAEQTQLLD
                     IAERENLLHLQRQRHLAAHPVVNANFQLHRPNRETVRALIRNTLDGVLTKAPILSKQI
                     VKELVGDLEQAAGILIDDKRLKAYLESKYYNRFNPEVEKAVFKALWKFAFRLTDEPCE
                     KNRAINYSALRLLFGRNPGQFKTQIEAEKDYFSAISTGGAPVVCLIDFLSREPQLYEL
                     LADHAKAAIQHAAESDISAHCLAWFTAANLQEHAKRLGDWISGKECPQIERMTWDAIE
                     KISDSPEWTKMVIRLANKYYVASGSYNGADRRFTEAIKPLLNKYELDDCVDLIEGIQC
                     NDQTWSRGRAGDDHRELRDRVLALDPKFEFSAYKVFTRYLNN"
     gene            complement(8713..11490)
                     /locus_tag="PLB25_00625"
     CDS             complement(8713..11490)
                     /locus_tag="PLB25_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOE40132.1"
                     /translation="MEVETQLQPGERITHHEFGQGVVLDPARDGYLRAFFGVGERRVP
                     VASVHRQLSRTERILRAVDGNADRARKAWLSYEAHALPVMESASALTSAKIDLLPHQV
                     VLTHRIATASPRRYLIADEVGLGKTIETALILRELASRGELTRALMVVPAGLVNNWHR
                     ELNEVFNLDFEVFGSEGDITDRKTNAFAKHDRLIASIDTLKRPARIKRLLDAPRWDLV
                     VFDEAHHLTAYRNGGKVRKTENYKLAEALKDHSRDLMLLSATPHQGNHFQFWMLAQLL
                     NPTLFGSPEEMLEHRHRLNTVMFRRTKADACQPDGSPLFARRWVHTESFLMGPEERLF
                     YEKLREYLEDGFDLARRQGGQGRALGFLMAIFQKIAASSFAAVRRTMKRRLLMLTLHE
                     AFLRDKDLDIEGRERLTDEARELIHEEFNLARDSIGRSEVDRVLADLKYRLVKKLDEE
                     ALELASDPYGSEYGAAHAEEAASAVVELHLPEERLRIGDLLKVFPQQRETKAQKLLDG
                     LGILWRQNPNEKIVVFATYLGTVDLIAREIDQTFPGQGVAVLRGGDHGAKVAAERRFR
                     QKDGPRVLVCTAAGREGINLQFARILFNFDLPWNPMDVEQRIGRIHRYGQNHTAQVYN
                     LVLSDTIEGRIFLMLDEKLVEIAKTVGKVDDQGNVAEDLRAQILGQLSERLNYDRLYQ
                     EALSDPELKRTKVELEAALSNSREARQVVFDLFQDLEGFSLDDYKPFSDVSSSLNRLV
                     RFMSAAVADRQQRLVKVDDETYDLVTVDGARKAGFTLNRETATSNDNVELMGLDHPLV
                     QEELGRWRIVPPEDLGIAVAADVDDPVLLSFWMVETSGKNGERRVVVQPIAVKQDGAR
                     VPALERQCERYLQAPTATPRFTPDQRINLFSQVVEPTLQRELKHKGAANGDGSYSAEL
                     IGYVEIVGQGA"
     gene            complement(11500..12000)
                     /locus_tag="PLB25_00630"
     CDS             complement(11500..12000)
                     /locus_tag="PLB25_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40133.1"
                     /translation="MKQLLDTVWQRRGTSWIWDEEARNQVCAASEVWSLRQFLQAAGH
                     WPDDLPSNGNNTLVVAGLEGGLDLLTPEDAETWLGDGVKDAILSFQSYYEGEASLIFW
                     LPSGLGRIKLHPATDSVEWRCAAPHSDRLLAFGRILWGEANEYPQEILLREGSKPAGL
                     FHLRIT"
     gene            complement(11997..15209)
                     /locus_tag="PLB25_00635"
     CDS             complement(11997..15209)
                     /locus_tag="PLB25_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HOE40134.1"
                     /translation="MATKKEQMAQEVAKAVGAGKTVALETVDFNDPNRPKTCLEVDFP
                     ILSVNQVATIEAASGAAKKPIYQMSKYWARRQSSVFRSMLIAAATKAPDDKSHAAKLV
                     WDNYYANHQKKGAFKHLKVADIFMGGGTTLVEGSRLGMQMVGNDLNPVAWFVVKQELA
                     NVEQEEVQSLLADIEAEVKPQIMPYYYCDGPEGETGTWTHLPTKLVKPADFDPLTIPR
                     EQRKDYRYDGPEVIYTFWAKHGPCQVTGCGHRTPIMTSPVVAVKSISVKHWEHTCGNC
                     GGEFHVEEDAARLAPDAPLYVVPSEPPFSVLDRRRGVTCPHCGHTAMLNLGKGKNKKV
                     ELSLLVHPQWLAGSPRQDANGQTCGGSAQDDLAATARWNKARASTSRLLEVRGTLPEE
                     VTCPETKVSFAPETGTVPRKSHYACAACGTVQDVLTTIKATGKTGPMAGYAVQGYSPK
                     RGAAGKPNSGRFFTGFATLQARQYDTAFSEWMARKDDDLKDYWPRSELPYGFMTHHLQ
                     GGVPNHGITHWWTMFNPRQLLVHAQLLKAICEIGDHAWSVREYVLCAFQQYLRCNNGL
                     SIWHMKNNQISAALSNNNFHPKSTSLEPSVFNQTGDGNWKSAIGGLIDALNWAADPWE
                     LVSAEGLARNHAELSSDISGKSEKVFPGDKMQAPEISCGSSTDLAPYTDASFDLVITD
                     PPFGGLLHYSELADFFYVWLRLVLKGKYPAIFGAEYTPKSLEAVANRAREPEDPDGFY
                     QRLLTQCWREAHRVLKPGGILAFTFHHSEDEPWVAVLESLFDAGYYLEATYPIRSDET
                     KGEGEFGSKTIEYDIIHVCRKRTEEPKPVSWGRMRREVMADVRQLQGMLENHAKEGLP
                     AADIQVIRRGKALEYFSRHYGKVFVDEGRAITVKEALIGINQLIDEDADKGKEPPPVN
                     AEPMTRQFLRTFGNATEMKRDQLQKFLKGSITTPDEFEQRGWCSEKSKVVTRVNPLDF
                     ARDWSGKHKRRLTSDLDQALVLIGACFDGSSINASDTLKNENFKPHVALKPLLEWLHK
                     NGPDQASRNAASRAVSIYNGWAATQAVKPTQGSLFEEYEL"
     gene            complement(15222..16244)
                     /locus_tag="PLB25_00640"
     CDS             complement(15222..16244)
                     /locus_tag="PLB25_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40135.1"
                     /translation="MALDVGVLNSLTKGSAEDAWRVIINNAVEIASKPLAPGNAASEV
                     IKRDREIGTLDHFLSSTGWDLWQTFGQPEISGVERTSDRLARWWTEPYSAKAVLMLDG
                     LSLRELPWLLQGAKERGFTLHEVSANASELPGETNQFAQALGFGSRSQLQNNGGGLAH
                     KLQPAQTECVDMPWKDCEGLINGTPGAKNWVFWHHWPDSKVHDGSGAGQGLDTLTRDA
                     ADQLSSDDFWSFVERLATGRRLVITSDHGYAATGYFPDADGEVGQFLKKTFASGRSAK
                     GTGLTQEDCGPFVPPVALQINSPHGAHLLAVGRWKWKSQGGYPTLTHGGLSLLEVLSP
                     FIELTK"
     gene            complement(16244..18988)
                     /locus_tag="PLB25_00645"
     CDS             complement(16244..18988)
                     /locus_tag="PLB25_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HOE40136.1"
                     /translation="MLGLTLREEFRGKRLKGTAIELSNDTNTGATQIAAKEFLDITYP
                     THDLLKGIEAVSPNQGRPVVVIGERGLGKSHLMAALYHAVNDAASTGAWLNSWATTLG
                     DPQISKIGLRSGMTVIGESLHRQRYKFLWDLLFERHPHGTYIKGKWEGMGAAKTDIPS
                     DKLILELLQHTPTMLLLDEFQTWYDGLTNTKQYPWKNWAFNFIQILSEIAKEHPELLV
                     LVISVRNGGSDAYQQVHRVNPVAIDFMAGGNPERVQQDRRRMLLHRLFTNRLQITKPQ
                     IASLIESHVAESLRLMEVPTAEHERKRKEFLVSWPFAPHLLRLLEEQVLIATDAQETR
                     DMIRILANLYKSRGEVAPVLTAADFRLDDDASGIGALLDSVSNEHHRTLRAKAQQNII
                     SVTEAVSDHANLAPHLQEIVGALWLRSIAVGNLAGAEPATLQVDITRNKAIDDNAFQV
                     ELATIVENSFNIHQDGARLVFKEEENPRAKLMACARNDKLFTDGSDQAQLAKQVRYVI
                     GGTDEVAKTFRVIALPKAWQTDPWVTLDEAEQPERWDDRLPVLVLPEEPDNIDQRLGR
                     WIKDHLQKRRNTIRFLLPRSGSTNAFQDRDLLILARAEMKAQEWSGQNPEYKKLHTEY
                     QGALRDILKKRFDRFAVLHRWNFADPNQCVFSVESLKKQGAQIPEGIEEALINDLFVP
                     EDFEALVLEAAADNSAVGKLLRELQEPRPAGQDCIPWLGETAMKERILRLCARGKIAI
                     NLRGLEYLQTQPGEDEDSAWKRLRPKLSYTGRQLDEVFLMEPSAVPTTGGTTPPAPQP
                     PTGGGEGGGLWGGGTTPPPPPPGGETTSGGGTPPPGGGIFGGGSSTGGKPRIPLSNPA
                     TSPLNLIGKLEGWGIGPATPVAEVSIKLSAATGAQLKELLKKLPDGMTFELSLEKEDK
                     "
     gene            complement(19039..19236)
                     /locus_tag="PLB25_00650"
     CDS             complement(19039..19236)
                     /locus_tag="PLB25_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009397209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE40137.1"
                     /translation="MTKPASDIMTITEVAAYLKVAERTLYRLAAAKKIPAFKVGGTWR
                     FSRADIDNWIKRQTMESHDTI"
     gene            19819..>20092
                     /locus_tag="PLB25_00655"
     CDS             19819..>20092
                     /locus_tag="PLB25_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40138.1"
                     /translation="MKYAYEDLSDGQFETLVVFLCQRLLGVSVHGFAKGPDGGRDAKF
                     VGTAELHPSRASPWAGTTIVQAKHTNGYNRNFSESDFFSTSAQNTVL"
BASE COUNT         3967 a         5756 c         5865 g         4404 t
ORIGIN      
        1 tgattgctgt gacgaccagc actttcacct atgacatcac tggcacgcct gtgagtccgg
       61 ctacaggtgc gagcatcacc tccaaggtgg cgcccctggg ctgggagatc gcttttacgg
      121 ccacaaacaa gcgcgcttac cgcagcaaaa atgtcctgtc caatcgtccc tacctgcggg
      181 tggacgatgg ttgtgatccg gcctacacca ccacttacgc caaaaaagcc aaagtgacca
      241 tggcccaagg catgagtgat attgacactt tcgtcggtgc ccgggctcca tttgacagtg
      301 cttaccccac acgaaacgaa atcgcgaccg gttccggtgc cggtgtatat gacggttggt
      361 acaagtggta ctacgccagg tcagatggca atgcctacga tcattacggc gcatctgttt
      421 tcaatcgccc ctggactttg gtcggtgatg atcgtggttt ctacatcttc aacgacacct
      481 ggagttcggg cggcctgggc ggcaagtgct tcacggactt cgagagttac cgcagtgccg
      541 acggtttcaa caccttgctc tgcgcgcagg aggcctataa ctatgcgtcc atacccagca
      601 gcagttatga cagcgaaggt tatcaatcct ccgactggcg ttcacgtttt ccacgaacgc
      661 tggacggaac cggcaagatt ctgatgcgca gctatctgca aattggcaat aacgtcaatc
      721 cctcgtttac cagcctaaac accaataatg gccagaccac ctcgggctac agcacgggca
      781 tcagttggcc caatgggccg gactacagca tgatcctgca cccaaccctg ctgcgcgaaa
      841 gctcccactt gcgcggcagg atgccgggca tgttctgggt gcataacgac agccccagtt
      901 tcaaccacct cgacaccatc tcgggcgtgg caggttaccc cgggcgcacc tttttgctgc
      961 tcaaggtcgc tcatggggtc agcagtggca actacaccgc cacactggcc tatgacatca
     1021 ccgggccatg gtggtaggcc atgagctggt ggataagtgc tgcggggctg accccgatca
     1081 ttgcttatga ctcctcacac ttccagtcga acgcggtttt gaccgatttg acgggtggtg
     1141 gcaaccatgc ggacatggcg ggccagtcgg ccagcctggt ggtggtggga accaacccca
     1201 ttcaatccat cacctgcgtc aaaggggtgg gtgctggctg gagttaccaa cgggtaactc
     1261 gtccgactgc tggcgtgctg atggcgctgg tgagtcacgt caatccacgg gtgatgctgt
     1321 tttctgatta cggggtgtct ggcaactacc atgcgctgct gattgagacg gatgtcacga
     1381 cactgagccc acatggacaa attcgctgcg aaggttcggc aaactctgtc ggactgccca
     1441 caggggcagc ggtcaagttc gtcgccatcg cttttgatgc aagcggctac cagtattact
     1501 gcaacggatc ctgggtgggg gcgaagttct ccgtgccact gagcttgctc tccagtgttg
     1561 gctccaattg gccatcatcc tggggcatgt acgcaaattt gtcggcactg ggcatgtttg
     1621 atggggttgc aagcctcgcc gatctgcaag acctggaagc tcaggttcgg ctggccgtag
     1681 tggggccacc tgtggttttt catgggtacg gcactgacct gagtcgggtc aacactatcc
     1741 caaccgtcaa tctggcaaca caaggtctgg gtgacaacac agggcgcatc aacaccgcac
     1801 caatgcaggc acttgatggt ctgggagttc gcaacaaagc gctggtgccg atcttgggcc
     1861 aacacgacat ttactttggt ggtgtcggcc aggtggtcgg caccgtcaaa aactcaccag
     1921 ccacaccagt acggcggcgt gttctgctga ttgaggaagc cactcgtgcc gttatccgtg
     1981 agacatggag cgaagcggcg accggtgcct attcgtttgg ccgggtcgcc atgaacacca
     2041 cctacaccgt ggtgagctac gaccacaccc aagcttttcg ggcggtggta gcggatcggg
     2101 tggttcctga agtgatgctg gagactgtcc catgacactg gccatttctc ctgcgcacaa
     2161 gctggcgcga ctgacaggga ctctttcgtt cgcggatgca ggcgcgcaaa acagccgcat
     2221 tcgactgtac gcaaccaccc agccatcgac tggtggcgat cctggcggtg ggttgctggt
     2281 ggaaatcctg ctggccaagc cgtgcggtga gatcaatgcc agcgtgctgg ccctcaagca
     2341 gtcagagcca tcaggtgacc tgattctgga gactggtact gcgctttggg cacgctggat
     2401 taacgggcgg gatgaagtgg tggccgacgg ttcggtgtcg gacgctgctg gtaccggaga
     2461 tttcaaactc tcgggcacca gcggcacggt gctttacgcc ggtgcgcggg cgctattggg
     2521 gcaatgcact ttgacttgat tgacttagat ttgtgaggtt acgcgtcgtg ccagaaaact
     2581 tggtctttgc tggtacaccg ctgattgaac agccgctgat gatggtgttt ggagagggtg
     2641 tgactgaccc gaacgccaaa atcaccgatc tggtgtttgc acagccgccc agggtgcatc
     2701 cgacccagtt ggtgttcggt gacagtgaca ccagcaccca cccggactca ttgctcgaag
     2761 tctcaggtgc gctgccagag ctggtcacac aattgcgact ggtcattggt gtgccggtgg
     2821 cgtggcaagc agcattgcct gatctggtcg gcacggtcgc tgtgaagtac cagagccaga
     2881 cgcaacggcc gatggtggcg caggtgcaga cctggtcgca ggtgtcgctg gtcaatgagt
     2941 ccggcctgac ccaaccacag cagcacgccc aagcaaccaa tgctggcgcg caagagcaag
     3001 cgcaaaacgc tgcctcgatc cgccagggtg tgagtccgag ttatgttgaa gccattcgtt
     3061 ccagccgaga ggttggatcg cagtttcagg atggatgcgc gacgcgatca cgcctgcatg
     3121 ccaattggtc ggacgggctg gctgaccggc gtgtccagag cacgggtccc ttccaggaag
     3181 gcctgcgtgc gccaacagtt cgcgccgtgg gccgttttga agacgggttg catgacaggc
     3241 gcgaaggaat agagaactct tgggctggcg cgattcctcg ggcgctgggt tactccggtc
     3301 acgctggtgc cgccatccca ctcaggcggt tcctgacgtc ggcctttcag gatgcttggg
     3361 tgccgcgacc cggtgtgcat ttgattccgg tgaccccgcc tgtagacccg acgtattggg
     3421 gcacagcctt gctgtttgcc tgtccgccat tggctgcacc gatgctggtg tttggggccc
     3481 gccagtgtga atttccagag gaaccgccaa tcggcgttgt gaccatagcg gtcagaaagg
     3541 tgtactttgt gatcaatgat gtcaatctgc gccgggcatc caacggggtg aacgtgccgg
     3601 tcaacagttt gtccctgtcg ctggatgcgt cttcctgggc ttggggattc gatgccgtgc
     3661 tgccgcacag tgcgcgaagc ctgattgagc ccaacggttc aggagtggtg gagctgctgg
     3721 ccaatgtgaa cggaaccact tttgcagtcc ttgctgaaaa catcagccgc gagcgcagct
     3781 ttgggcagac cagcattcga ttgactggtc gtggcaagaa tgccgtgctg gccgcaccct
     3841 atgcgccggt gatgacgttt accaacaccc aggctcgcac ggcacgccag ttgatggacg
     3901 acgtgttgac catcaacggt gtgcctatgg gttggaatgt ggactggggg ttgaccgact
     3961 ggaatgtgcc agccggggtg ttttcgcacc aaggaacgtg gatcgaggcc atgagtgtca
     4021 ttgccggggc agctgggggc tatctgctgc cgcaccccag agaccaaacg attcgcgtcc
     4081 gacaccaata tccggttcca ccctgggagt ggtccacagc gacccccaac tttgtgctgc
     4141 cagtcgatgc cgtggaaaag gaatccctgc gctggttgga aaaaccgtcc tacaaccggg
     4201 tgttcgtctc tggccaggag gcaggagtgc tggcgcaggt cactcggtcg ggtaccgccg
     4261 gagaacaatt ggcaccgatg gtggtggatg ccctgatcac tgaggcggca gttgcccgcc
     4321 agtgtggtct ggcagtgttg agcaacaccg gtcggcagat tgaggtcagt ttgaatctgc
     4381 cggtgctgct tgagaccgga atcattgaac ccggtgccct cgtccagtat cgagatggcg
     4441 gtattgaccg cattgggctg gtgcgatcca cccaagtcca ggccgggttt ccagaggtct
     4501 ggcaaacctt gggagtgcag acctatgcat aacgtctatg agcagttcaa gcagctgctg
     4561 tctgacccac cgttacaggt cggcaccgtg tcaaaagtag gcacaggcgt tgtcacggtt
     4621 cagttgcccg gtggcggcac cgtcaaggcc agaggcagcg cagatttcgg tgcgcgcgtc
     4681 tttgtgcgcg atggcgttct ggaagccgtt gcacccaatc tgagtctgga aatcatagaa
     4741 atctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngatggc gttctggaag
     4861 ccgttgcacc caatctgagt ctggaaatca tagaaatcta atcccgactg aaaagtaatt
     4921 tttaaccttt tttaacccgt tacccgcttc ggtgtgcatc acgcgcatct ggcgggtttc
     4981 gcatttttgg agcatgaaaa atggaagatt taccaattga acggcgcaag acggtcacga
     5041 tgccgcaggc agactttgag cagatgcttg agcgtgcggc cgaaagaggt gcgcgtcacg
     5101 ccctgcatgg ggtgggactc gatggcgagg acgctgctca cgacattcgc gaactgcgaa
     5161 atctgctcga tgccttcaat gaagccaaga agactgccgg tttgaccatc atcaagatga
     5221 tcgtcaccgg actggtaatg gcgatcctga ccggcaccct gatcaaactc aaattgttcg
     5281 ggggtgggca atgatcgaaa cactgctagg tggattgttg ggtggagcgt ttcgactggc
     5341 accagaactt ctgaaatggc tggaccgcaa gggcgaacgt ggtcacgaac tggcgatgca
     5401 agacaaggcg ctggagttcg agaaaattcg tggagccagt cgaatgggag agatcggtgc
     5461 aagtgccgac gctgcttgga actccggtgc gatggaggcg ttcaaggaag cggtttcggg
     5521 tcaaggcaga ccatctggtg tgaagtgggt ggatgcactg tcgtccacag ttcgcccgat
     5581 ggtgacttac ctcttcgtgc tgatgtacgc cggtgtgaaa ctgtccacct tcgttgggtc
     5641 ggtgcaaacg ggtatggggt tcggggacgc gctgctcgcc gcgtggacgg aagcagacca
     5701 ggcattgctt gcaggcatcc tgaactactg gtttctgaat cgcacaattg agaaggggct
     5761 gcggtgatgt ggcggcaaaa cgatccattg aagtcatgga gtcgcgcacc acgaccattg
     5821 gcagaacgat tctgggaaaa ggtggatgtg cggggtgctg atgaatgctg gccgtggctg
     5881 gcatccaaaa agcaaggtgg ctacggtcgg atcagtgatg agcgcggcca tcctcagctt
     5941 tctcatcgcg tgagttacgc gctgactgtc agtccgattc ctgatggaaa cgtggtctgc
     6001 catcgctgcg acaacccacg ttgcgtgaat cccgcccacc ttttcctcgg tacacaagcc
     6061 gacaacctgc gagatatgcg tagcaaggga cgtggcaacc cgccccgtgg tgtcgagcat
     6121 ccgaaggctc gattgacgga agcactcgcg cagcagatac ggcgcgattc tcgcagccat
     6181 cgtcagctcg ctcgcgcatt cggcattggc aagtcaacag tcggaatgat caaagcgggg
     6241 gacacatgga cactcgtgtg attggcgttc cgccgcaggc cattgagctc gcgaagcgat
     6301 tcgaggggtt tcaccgtgtg cccaagaacg atcccttggg ccaggcgcat ccttacatct
     6361 gcccggcagg attttggaca gttggctacg gccacctttg cgaccccaag cacccaccga
     6421 tcaccgaggc tgaagccgag gtctatttgg cccaagacct gatgacggcg ctcaccgcca
     6481 ctttgcggta ttgtcctgta ctggcaacgg agtctgagtc gcggcttgca gccatcgtgg
     6541 atttcacatt caatctaggg gcgggtcggc ttcagacatc gacgttgcgt cggcggatca
     6601 accagcttga ttgggcaagc gctggtcagg aactgcgccg ctgggtctat ggcggtggga
     6661 aggttcttcc aggactcgtc aaaaggcgcg aggcagaagc tttgcggctg atttagacag
     6721 cacgtttcgc ttatcaactc taccgacttc gggataagat tcaaaccttg ccatctctcc
     6781 gttttgcacg atgatcaatt ggaaagaagc cccaaagaca gcccgatggt gggcgatgga
     6841 tgccgacggg caggcgcact ggtatctcgc accaaatgtc gctgccttca ccacgttctg
     6901 gtattgcgat caggttctgg cacccagcta cgactttgct ggagattggc gcgaaagttt
     6961 gactgagcga ccgccccacc cacgaccagt cgtaagctga taattttcga ttcgccgtca
     7021 ttcaaacctg agctggacat tccccctacg tctgcccggt tgggcatcac cgcatcgggt
     7081 ttaggcacct gtgcgagcca gaccatccgc taatcaccga gactgaagcc gaagtctatt
     7141 tgactcaaga cttggtgtct gcgcttgccg ccacacttcg gtacttccca gtgctggcta
     7201 ccgattccaa cgagcgactc gtggccatcg cagacttcat attcaccttt ggggctgggc
     7261 tgctccagac atcagcgcta aggcgacgaa ttaaccagct aaactggtca gaaattgctc
     7321 agaagtttcg gcgatgggtg catagcggcg gtaaggtact cccagggcta gtaagcaggc
     7381 gcgaggcgga gctgcgtttg ctggactatt agttgttcaa atatcgggtg aataccttgt
     7441 atgcagagaa ttcgaacttt ggatcgagtg caagaacacg atctcgcagc tctctgtgat
     7501 catcaccagc acggccacgg ctccaagtct gatcgttgca ctgaattcct tcaataagat
     7561 caacgcaatc gtcaagttca tacttgttca ataagggctt gatagcctct gtgaagcgcc
     7621 gatctgcacc gttgtagctc ccgctggcaa cgtagtactt gtttgcaagg cgaatcacca
     7681 tcttggtcca ttcgggcgaa tccgatattt tttcaatggc atcccaggtc atccgctcga
     7741 tttgaggaca ctccttgcct gaaatccaat caccaagccg ttttgcatgt tcttgcaggt
     7801 tcgcggcagt gaaccacgcc agacaatggg cagaaatatc gctctcggcc gcgtgctgaa
     7861 ttgctgcttt ggcgtgatca gccaatagtt cgtaaagctg tggttcgcgt gacaggaaat
     7921 ctatcaagca gacgaccggg gcaccaccgg ttgaaatcgc gctgaagtaa tccttttctg
     7981 cctcgatctg cgtcttgaac tggccaggat ttctcccgaa cagcaagcgc aaagcgctgt
     8041 aattgatcgc acggttcttc tcgcacggct cgtcagtcag acgaaatgca aacttccaga
     8101 gagccttgaa gactgccttc tccacttcgg gattgaaccg gttgtagtac ttgctctcca
     8161 ggtacgcctt caacctcttg tcatcaatca gaatgccggc tgcctgttcg agatcaccga
     8221 cgagctcctt gacgatctgc ttggacaaga tcggggcttt ggtcagcacc ccatcgagag
     8281 tgttacggat caaggcgcgt actgtctcgc ggttgggccg atgtagttgg aagttcgcgt
     8341 tgaccaccgg gtgagcggcg agatgacgct gacgctgcag atgcagcagg ttttcccgct
     8401 cggcgatgtc aagaagctgc gtctgctcgg cgacggattc gacgagcttc gtctcccaaa
     8461 tcggagatcg ttcgttgtcc tgctgcagct tgccgatttc cgcaaggatg tccttggctt
     8521 tggtgtcgcc gtatagatcg acgaggttct gcagcttgaa gagcaggtcg caaaccgcga
     8581 ccgaccaaag catcactacc gatgaccggt agttgcctgc cgcgtagcag ctcaatacct
     8641 cggcgaagta ttccttagta cgcgaatcga agatttgcgt tgcgcgctgc tcaattgagt
     8701 attcgtccag catcacgccc cctgaccaac gatctcgaca tagccaatta actcggccga
     8761 atagctgccg tcgccattgg ccgcgccctt gtgcttgagc tcgcgctgca gcgttggctc
     8821 aactacctgc gaaaagagat tgatccgctg gtccggagtg aatctcggcg tggctgtcgg
     8881 ggcctgcagg tagcgttcac attgccgctc caacgcaggc acccgcgctc cgtcttgctt
     8941 gaccgcaatc ggctgaacca ccacgcggcg ctcgccgttc ttgccggagg tctcgaccat
     9001 ccaaaatgac agcagtactg ggtcgtcgac gtcggccgca acggcaattc caagatcttc
     9061 cggcggcacg atgcgccagc ggccaagctc ttcctgcacc agtgggtgat ccagtcccat
     9121 cagctccacg ttgtcgttac tcgtggccgt ctctcgattc aacgtaaatc cggccttgcg
     9181 cgcaccatcg acggtcacaa ggtcataggt ctcgtcgtcc actttgacga gccgctgctg
     9241 gcgatctgcc accgctgccg acatgaatcg caccaatcga ttcaggctgg acgacacatc
     9301 agaaaagggc ttgtagtcgt cgagactgaa gccttcgagg tcttggaaca ggtcaaacac
     9361 tacctgccgc gcttcgcgcg aattcgacag ggcagcttcc aactccacct ttgtccgctt
     9421 gagctcgggg tctgatagcg cctcttggta caggcggtcg tagttgagcc gctcggaaag
     9481 ttggccaaga atctgtgccc gcaggtcttc ggcgacgttg ccttgatcat cgaccttgcc
     9541 gaccgtcttg gcgatctcga ccagcttctc gtccagcatt aggaagatgc ggccttcgat
     9601 ggtgtcggac agcacaaggt tgtagacctg tgccgtgtgg ttctgtccgt agcggtggat
     9661 gcgaccgatt cgctgctcca cgtccatcgg gttccacggc aaatcgaaat tgaacaggat
     9721 gcgcgcgaac tgcaggttga tgccttcacg accagcagcc gtacaaacca gcacgcgcgg
     9781 gccatccttc tggcggaagc ggcgctccgc tgcaaccttg gcaccgtgat caccaccacg
     9841 cagcacagcc acgccctggc cagggaaggt ctggtcgatc tccctcgcaa tcaagtcaac
     9901 ggtgccgagg taggtggcaa acacaacgat cttctcgttc gggttttgcc gccacaggat
     9961 gcccaagccg tcgagcagct tctgcgcctt tgtctcgcgc tgctgcggga agaccttgag
    10021 cagatctcca atgcgcagcc gttcttccgg caggtgcaac tccacgacag ccgatgcggc
    10081 ttcttcagcg tgggcggctc catattcgct gccataagga tcggaggcca gctccagcgc
    10141 ttcctcgtcc agcttcttga ccagtcgata cttcaggtcg gccagcacac ggtccacctc
    10201 gctccggccg atgctgtcgc gtgcaaggtt gaactcttca tggatcagct cgcgggcttc
    10261 atcggtcagc cgctcgcgcc cctcgatgtc gaggtctttg tcgcgcagga atgcctcgtg
    10321 cagcgtcaac atcagcagtc ggcgtttcat ggtgcgccgc acggcagcaa agctcgacgc
    10381 agcaatcttc tgaaagatgg ccatcaagaa gccgagggca cgcccctggc cgccctggcg
    10441 acgcgcaagg tcgaagccat cctccaggta ctcgcgcagc ttctcgtaga agaggcgctc
    10501 ttccgggccc atcagaaagg actcggtatg cacccagcgt cgtgcaaaca acggcgaacc
    10561 gtccggctga caggcgtccg cctttgtgcg acggaacatc accgtgttca ggcggtgccg
    10621 gtgttccagc atttcctcgg ggctgccgaa tagcgtaggg ttcagcagtt gtgccagcat
    10681 ccagaactgg aagtggttgc cttggtgagg cgtggccgag agcagcatca ggtcgcgcga
    10741 atggtctttc agcgcttcag ccaacttgta gttctcagtc ttcctgacct tgccgccgtt
    10801 gcggtatgcg gtgaggtgat gcgcttcgtc gaacaccacc agatcccaac gcggcgcatc
    10861 cagcaggcgc ttgatacgcg ccggccgctt cagggtgtcg atgctggcga tcagccggtc
    10921 gtgcttggcg aaggcgttag tcttgcggtc agtgatgtcg ccctcggagc cgaacacctc
    10981 gaagtcgagg ttgaacactt cgttcagctc gcggtgccag ttgttcacca gacccgcagg
    11041 caccaccatc agcgcccggg tcagttcgcc ccggctggcc agctcccgaa gaatgagtgc
    11101 cgtctcgatg gtcttaccca gcccgacctc gtcggcgatc aggtatcgcc ggggcgacgc
    11161 ggtggcgatg cggtgggtca gcaccacctg atgagggagc agatcgatct tggcggacgt
    11221 cagtgccgac gcgctctcca tcaccggcag tgcgtgcgcc tcgtaggaca gccacgcctt
    11281 gcgtgcacgg tcggcgttgc catcgaccgc acgcaagatg cgttcggtgc gagagagttg
    11341 gcggtgaacc gaggcaaccg gcacacggcg ctcaccaacg ccgaagaacg cgcgcaggta
    11401 gccgtcgcgt gccggatcaa gaacgacgcc ctggccgaat tcgtgatggg tgatccgttc
    11461 gccgggctgg agctgagtct ctacttccac gcgccgacct caggtgatcc gcaaatggaa
    11521 caagccagca ggcttgctgc cttcgcggag caggatctcc tgcggatact cgttggcctc
    11581 gccccacaga atgcggccga acgcgagcag gcggtcgcta tgcggtgcag cgcagcgcca
    11641 ctcaaccgag tcggtggcgg gatgcaactt gatccgtccg agacctgatg gcagccaaaa
    11701 gatcaaggac gcttcaccct cgtagtagga ctgaaacgac aggatcgcat ccttgacccc
    11761 atcgccgagc cacgtctcgg cgtcctcggg cgtcagcaga tccaggccac cttccaaccc
    11821 ggcaacgacg agcgtgttgt tgccattgct cggcaggtcg tcgggccaat ggcccgccgc
    11881 ctgcaagaac tggcgcaggc tccagacttc gctggccgcg cacacttgat tgcgggcctc
    11941 ttcgtcccaa atccaactcg tgccgcgccg ctgccacact gtgtcgagca gttgcttcat
    12001 agctcgtatt cctcaaacag cgaaccctgc gtcggcttga ccgcctgagt cgccgcccag
    12061 ccgttgtaga tcgagaccgc gcgtgaggcc gcgttgcggc tggcttgatc cgggccgttc
    12121 ttgtgcagcc attccagcaa gggcttgaga gccacatggg gcttgaaatt ctcgttcttc
    12181 aaggtgtcgg aggcgttgat gctactgcca tcgaagcagg ccccgataag taccagcgcc
    12241 tgatccaaat cggacgtcag tcttcgcttg tgcttgccag accagtcgcg ggcaaagtcc
    12301 agcgggttca cccgcgtgac gaccttgctt ttctctgagc accagccacg ctgctcgaac
    12361 tcgtcgggcg tggtgatcga gcccttgaga aatttctgga gctggtcacg cttcatttcg
    12421 gtggcattgc cgaaggtgcg caagaactgg cgggtcatcg gctcggcatt gaccggcggc
    12481 ggttccttgc ccttgtccgc gtcctcatcg attagctggt tgataccgat caacgcttct
    12541 ttgacagtga ttgctcgccc ctcgtccaca aagaccttgc catagtggcg cgagaagtac
    12601 tccagcgcct taccacgccg gatcacctga atgtcggcgg caggcaggcc ttcctttgcg
    12661 tggttttcca acataccctg cagttggcgc acgtcagcca tcacctcgcg gcgcatccgg
    12721 ccccaactca caggtttggg ttcttcggtg cgcttgcggc agacgtggat gatgtcgtac
    12781 tcgatggtct tcgagccgaa ttcgccttcg cctttggttt cgtcggagcg gatgggatat
    12841 gtcgcttcga ggtagtagcc cgcgtcgaag agagactcta gcaccgcgac ccatggctcg
    12901 tcctcgctgt ggtggaaggt gaatgcgaga atgccgccgg gcttcaagac acgatgtgcc
    12961 tcgcgccagc attgggtgag caggcgctgg tagaagccat ctggatcttc tggctctcgg
    13021 gcgcggttgg ccacggcctc cagcgatttg ggcgtgtact ccgccccaaa gattgcaggg
    13081 tactttcctt tgagcactag gcggagccag acgtagaaaa agtcggctag ttcggaatag
    13141 tgaagcaagc cgccaaaagg cggatcagtg ataaccaaat cgaaacttgc atcggtatac
    13201 ggagcaaggt ctgtcgatga cccacaagat atctctggcg cctgcatttt gtcgcccgga
    13261 aatacctttt cgctcttgcc ggaaatgtca ctggataact cagcatgatt tctagccaat
    13321 ccttccgcac taacaagctc ccatggatca gctgcccagt tcaatgcatc tattaaccca
    13381 ccaatggcag acttccaatt gccatcgcca gtctgattaa aaacacttgg ctccaaactt
    13441 gtagattttg ggtggaaatt gttattacta agggcggcag atatttggtt attcttcata
    13501 tgccaaatgc tcaagccatt gttgcagcga agatactgct gaaatgcaca cagcacatac
    13561 tcccgcacac tccatgcgtg gtcaccgatc tcacaaatag cttttagtag ctgagcatgg
    13621 actagtaact ggcgcggatt gaacatcgtc caccagtgag tgatgccgtg gttgggcaca
    13681 ccaccttgca gatgatgggt catgaaaccg taaggaagtt ctgaccgtgg ccagtaatct
    13741 ttgagatcat cgtccttacg cgccatccac tcactaaacg ctgtgtcgta ctgccgcgct
    13801 tgtaacgtcg cgaaacccgt aaaaaagcga ccactattag gtttgcctgc tgcacctcgc
    13861 ttgggcgaat aaccttgcac cgcatacccg gccatcggcc cagtctttcc cgttgccttt
    13921 attgtggtta acacatcctg cacagtaccg catgcggcac atgcgtaatg tgatttcctc
    13981 ggcaccgtgc cagtttccgg cgcgaaactg actttcgtct cagggcacgt cacctcttcc
    14041 ggcagcgtgc cgcgcacttc cagtaggcga ctcgtagatg cacgcgcctt gttccaccga
    14101 gcagtcgcgg ctaaatcatc ctgcgccgat ccaccgcagg tctggccatt cgcatcctgt
    14161 ctgggcgagc cagccagcca ttgcggatgc accagcaggc tcaattcaac cttcttattc
    14221 ttccctttac cgaggttgag catcgccgta tgcccgcagt gagggcaagt aacgcctctc
    14281 ctccgatcaa gcacggagaa agggggctca gatggcacca catacagcgg cgcatcaggg
    14341 gccaagcgcg cagcgtcttc ctcaacgtga aactcgccac cgcaatttcc gcaggtgtgc
    14401 tcccagtgct tgacgctgat cgacttcacc gcgactactg ggctagtcat gatcggggta
    14461 cgatgaccac agcccgttac ctgacaaggg ccgtgctttg cccagaaggt ataaatgact
    14521 tccgggccgt cgtagcgata gtcctttcgc tgctcgcgag gaatggtcaa gggatcgaaa
    14581 tcggcaggct ttaccagctt ggtcggtagg tgcgtccatg tgccagtctc accttccggc
    14641 ccgtcgcagt aatagtacgg catgatctgc ggtttgacct cggcctcaat gtcagcaagc
    14701 agactctgga cctcttcctg ttcgacattg gccagctctt gcttgaccac gaaccatgca
    14761 actgggttaa ggtcgttacc aaccatctgc atgccgagac gtgagccttc caccagcgtg
    14821 gtgccgccgc ccatgaagat gtcggccacc ttcaaatgct tgaacgcacc tttcttctgg
    14881 tggttggcgt agtagttgtc ccagaccaat ttggccgcgt gcgatttgtc atctggggcc
    14941 ttggttgcag ccgcaatcaa cattgaccgg aagacgctag attgccgcct tgcccagtat
    15001 tttgacatct gatagatggg ttttttcgcc gctcctgatg ccgcttctat agtggccacc
    15061 tggttgaccg acagaatcgg gaaatccacc tctaagcagg tcttcgggcg gttcggatcg
    15121 ttgaagtcga cagtttcaag cgcgaccgtc ttgccagcgc cgacggcctt ggcgacttct
    15181 tgcgccatct gttctttttt ggttgccatt cagcgatttc cttatttcgt gagctcgatg
    15241 aagggcgaca gcacctcgag caatgagagg ccgccgtgcg tcaacgtcgg gtagccgcct
    15301 tggctcttcc acttccagcg gccgacggcc aggaggtgag cgccgtgcgg gctgttgatc
    15361 tggagtgcca cgggcggcac gaaggggcca caatcttctt gtgtgagacc ggtgcccttg
    15421 gcgctgcgcc cgctggcgaa ggtcttcttg aggaactggc caacctcgcc atccgcgtcc
    15481 gggaagtagc ctgtggcggc gtagccgtgg tcggaggtga tgaccagacg tcgcccggtg
    15541 gccaagcgct cgacgaaaga ccagaagtca tcgctgctga gttgatcggc tgcgtcgcgg
    15601 gttagcgtgt cgaggccctg gcccgcgcct gatccgtcgt gaaccttgct gtcgggccag
    15661 tggtgccaga acacccaatt ttttgcgccg ggcgtgccgt tgatcaggcc ctcgcaatcc
    15721 ttccacggca tatcgacaca ctcagtctgc gccggttgta gcttatgggc aagaccgccg
    15781 ccgttgttct gaagctggct gcggctgccg aagcccaacg cctgcgcgaa ctggttggtt
    15841 tcaccgggca gttcggatgc gttggcggat acctcgtgca gcgtgaagcc gcgctccttg
    15901 gcaccttgta gcagccaagg cagttcacgc agggacaagc catccagcat cagcaccgcc
    15961 ttggcgctgt agggctcggt ccaccagcgg gccagtcgat ccgacgtgcg ctcgacgccg
    16021 cttatttcag gttggccgaa ggtttgccac aaatcccagc cggtggagga caggaagtgg
    16081 tcaagtgtgc cgatttcgcg gtcgcgcttg atgacctcgc tggctgcatt gcctggagcc
    16141 agtggcttgg acgcaatctc gacggcgttg ttgatgatga cgcgccacgc gtcctcggca
    16201 gagcctttag tcaggctgtt caataccccg acgtcgagtg ccatcacttg tcctctttct
    16261 caaggctgag ctcaaaggtc ataccgtcag gcagcttctt gagcagttcc ttgagttgcg
    16321 ccccggtcgc tgccgacaac ttgatcgaga cttcggcgac gggggtggcc gggccaatgc
    16381 cccaaccctc gagcttgccg atcagattca agggagaggt cgcaggattg ctcagcggaa
    16441 tgcggggctt gccaccagtg ctggagcctc cgccgaagat tccgccgccc ggcggcggcg
    16501 tgcctcctcc cgatgtcgtt tcaccgcccg gtgggggcgg cggtggcgtt gtgccgccac
    16561 cccaaaggcc gccgccttcg ccgccgccag ttggcggctg aggcgcgggg ggcgttgtgc
    16621 cacccgtggt cggcaccgcc gagggttcca tcaggaacac ttcatcgagc tggcggccgg
    16681 tgtacgacag cttggggcgc aagcgcttcc aagccgaatc ttcatcctcg ccgggctggg
    16741 tctgcaagta ttcaaggccg cgcaagttga tggcgatctt gccccgcgca cacaggcgca
    16801 ggatgcgttc cttcattgcg gtttcgccaa gccaagggat gcagtcttgg ccagcaggac
    16861 gaggctcttg cagctcgcgc agcagcttgc caacggctga gttgtcggca gcagcttcga
    16921 gcacaagggc ttcaaagtcc tctggtacga acaggtcgtt gatcagcgcc tcctcgatgc
    16981 cttcggggat ctgagcccct tgcttcttca ggctctcgac gctgaatacg cactggttcg
    17041 gatcagcgaa attccagcga tgcagcaccg caaaacggtc gaagcgcttt ttcaggatgt
    17101 cgcgcagcgc accttggtat tcggtgtgca gcttcttgta ctctgggttc tggccgctcc
    17161 attcctgtgc cttcatctcg gcacgcgcga ggatcagcag gtcgcgatcc tggaaggcat
    17221 tggtggagcc ggaccgcggc agcaggaagc ggatggtgtt acgacgcttc tgcaagtgat
    17281 ctttgatcca gcggcccagg cgctggtcga tgttgtctgg ttcctctggc agcaccagaa
    17341 cgggtaggcg gtcgtcccag cgttccggct gctcggcttc atccagcgtg acccaaggat
    17401 cagtctgcca agctttcggc agcgcgatca cacggaacgt cttggcgacc tcgtcggtgc
    17461 caccgatcac gtagcgaacc tgcttggcaa gctgggcttg gtcggaaccg tcggtgaaca
    17521 gcttgtcgtt acgagcacaa gccatcagct tggcacgcgg gttttcttct tccttgaaga
    17581 ccagccgggc gccatcttgg tggatgttga aactgttctc gacgatggtc gccagctcga
    17641 cctggaacgc gttgtcgtcg atggctttgt tgcgcgtgat gtcgacctgc agcgttgccg
    17701 gttcggcacc agccaagtta ccgacagcaa tggatcgcag ccacaaagcg cccacgatct
    17761 cctggaggtg cggggcaagg ttggcgtggt cggacaccgc ctcggtgacc gaaatgatgt
    17821 tctgttgggc cttcgcgcgc agggtgcggt ggtgctcgtt ggagacggag tcgagcagcg
    17881 cgccgatgcc cgaggcatcg tcatcaagac ggaagtccgc agccgtgagc actggtgcca
    17941 cctcaccacg actcttgtag agattggcca ggatgcgaat catgtcgcgc gtttcctgcg
    18001 cgtcggtcgc aatcagtacc tgctcttcaa gcaggcgcag caggtgcggg gcaaacggcc
    18061 aagagaccaa gaattccttg cgcttgcgct cgtgctcagc ggtgggtacc tccatcaagc
    18121 gcaagctctc ggccacgtga gattcaatga gcgaggcgat ctggggcttg gtgatctgca
    18181 gccggttggt gaacagccgg tgtagcagca tccggcgtct atcttgctgt actcgttcgg
    18241 ggttgccgcc cgccatgaag tcgatggcga ccggattgac gcgatgcact tgttgatagg
    18301 cgtcgcttcc accgttgcga accgaaatca cgagcaccag caattccgga tgctccttgg
    18361 cgatctcgga caggatctgg atgaagttga acgcccagtt cttccagggg tactgcttgg
    18421 tgttagtcag gccgtcgtac caagtctgga attcgtccag cagcagcatc gtcggcgtgt
    18481 gctgcaacag ctcgaggatc agcttgtctg acgggatgtc agtttttgcc gcgcccatgc
    18541 cctcccactt gcccttaatg taagtgccgt ggggatggcg ctcgaacagc aagtcccata
    18601 ggaacttgta gcgctgccgg tgaagactct cgccgatcac tgtcattccg ctgcgcagcc
    18661 cgatcttgct gatctgcggg tcacccaagg tggtggccca cgagttcagc cacgcccctg
    18721 tcgaggctgc atcgttgact gcgtggtaca gagcggccat caagtgagac ttgccgaggc
    18781 cccgctcacc aatcacgacc acaggacgcc cttggttcgg gctaaccgcc tcgatcccct
    18841 tcagcaggtc gtgggtcggg tacgtgatat ccaaaaactc cttggctgcg atttgagtcg
    18901 caccagtgtt tgtatcgttg gagagctcaa tggctgtccc tttgaggcgt ttaccccgaa
    18961 attcttcacg tagtgtcagt ccaagcattg ttacttctcc ctattagtga tttggcgttt
    19021 ggccgtgttt tcattttctc aaatggtgtc gtgcgattcc atcgtttggc gtttgatcca
    19081 gttgtcaatg tctgcgcgcg aaaaccgcca tgtcccgccg accttgaacg ctggaatctt
    19141 tttggcagcc gccagacggt aaagagttcg ctccgccacc ttgaggtacg cggcgacttc
    19201 agtaatcgtc atgatgtctg atgcgggttt cgtcattgca aaaactgtcc ctgttgtcaa
    19261 aattggctaa tttcggcaac atcttacccc tgaacagaac ggtcaaaaat ttgggtaagt
    19321 tggtgggcag atgccaacat gactggcatg cgttggcagc cttgaatttg cagcattttt
    19381 gtctagccac agacgattgc gcatcaccgg cgtttggcga cacgccaaga aaaaacccag
    19441 cggattaggc tgggttgaac ccttgcgggc cccgttcagg gaggagaaac ggaggggctt
    19501 taaacccgaa cgcaatgtat cacgtctcca ggttcacttt gaacgcattt ttcatcggtt
    19561 tatccagtcg tactgaaagc tatcaacttc agtgattcgc tccagatttc atccttaaac
    19621 cgccaagcta gacgcacgta caggcataat caagggtttg aattctctgg cgagttgttg
    19681 actaaatcgg agatcatcat gcaaaatatt tacggccaaa tgtcaatgat tgcgaccttc
    19741 ggtgacattc aaacgccaca ggaatcatgg tgactgcatg cttcgcccag gtgaaatgca
    19801 gctgatttcg agtgcttaat gaagtacgcc tacgaagatt tgagcgacgg acagtttgag
    19861 acattggttg tttttttatg tcaaaggctg ctcggcgttt cagtgcatgg tttcgcaaag
    19921 gggccagatg gtggacgcga tgccaaattc gttggcacag ccgaacttca tccgagcaga
    19981 gcatcgcctt gggctggaac aacgattgtt caagcaaagc acacgaacgg ctacaaccgg
    20041 aatttttcgg aatccgattt tttcagtacg tcagctcaaa acacagtact tg
//
LOCUS       DAOSLL010000008        18986 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_3139_length_18986_cov_4.237917, whole genome shotgun sequence.
ACCESSION   DAOSLL010000008 DAOSLL010000000
VERSION     DAOSLL010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 18986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18986
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3139_length_18986_cov_4.237917"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..596
                     /locus_tag="PLB25_00660"
     CDS             102..596
                     /locus_tag="PLB25_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HOE40139.1"
                     /translation="MQQTLKLIAGGADGTGARGLDLGFCQNVIELANDADPRALTLDG
                     VTRIDLKFPKFTDGRAYSQAFLLRRRLGFTGELRATGDVLVDQLQQMQRSGFDTAVLR
                     ADQNLATAQRQLALFDHFYQGDAVHPQPHFTPATDQAGAPKGTLPSALQPGSNAQPTH
                     GASA"
     gene            593..1381
                     /locus_tag="PLB25_00665"
     CDS             593..1381
                     /locus_tag="PLB25_00665"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HOE40140.1"
                     /translation="MNAITLNARPSATFTAKLQETTALLQQAALDYAANTPGSASRIT
                     LACSLGAEDMVLASLINQLQLDIGIFVLETGQLHPETLALLARLQASSRAPVEVFRPV
                     EHDVLQFVSQNGKDAMYQSIALRKACCGIRKMEPLGRALAGKAAWITGLRREQSGSRA
                     EVPLIDRSDASIVKFNPLGHWSWGDVWHFIAVNQVDYNPLHDQFYPSIGCAPCTRAIS
                     AGEDFRAGRWWWEDEAAKECGLHVAGGPLHSLSAQRSDLAQVAA"
     gene            1414..2352
                     /gene="cysD"
                     /locus_tag="PLB25_00670"
     CDS             1414..2352
                     /gene="cysD"
                     /locus_tag="PLB25_00670"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HOE40141.1"
                     /translation="MSHHHHRPDHLSNAHLDALEEETIFILREVAAAFERPTLLFSGG
                     KDSLVMLKCAEKAFGTPATGGKIPYPLLMIDTGHNFHEVTDFRDFRAKELGAELIVRS
                     VEDSMARGTVRLAHPGESRNVHQSVTLLEAIDEFRFDALIGGARRDEEKARAKERIFS
                     HRDSFGQWQPKDQRPELWTLFNTKLHPGEHFRVFPISNWTELDVWQYIAREHIALPSL
                     YYTHQREVVERKGLLVPVTELTPLKPGEVAELRDVRFRTVGDITCTCPVESLAATPEQ
                     IVLETLAADVSERGATRMDDKTSEASMEKRKKDGYF"
     gene            3099..4472
                     /locus_tag="PLB25_00675"
     CDS             3099..4472
                     /locus_tag="PLB25_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOE40142.1"
                     /translation="MNTTTNIATHADSTSATAQNDHQSALKFITCGSVDDGKSTLIGR
                     LLIDSRSVLQDQLANVSKSGEADLAQFTDGLSAEREQGITIDVAYRYFNTAQRKFIIG
                     DAPGHEQYTRNMVTAASSADAAVVLVDATKLQWQDPALELLPQTRRHALLVNLLRVPS
                     IVFAINKLDALEDGATLAFTNIRAALEEFASAAGIEIATVVPISALKGHNVVDTVDGW
                     CGYTGPSLLDILELLPNTPADSALPLALPVQWVEKFSSSSDTRQGRRIFWGRVASGLA
                     MVGQMVKVLPSGQTATVAQVLGHTRQPGTVQAGHSAGIVLDREVDVSRGDWLLAFAAS
                     AAAATADDDFDTPSAPTSLPPSRELAVTVAWMDDEPLVAGRVYWALHGHRWVKARVRR
                     VVHRLDINTLAEDDAEALPANAIGHVELLLQEPIAARPFAQSRVLGSLVLVDTASHRT
                     AGAALVN"
     gene            4664..4993
                     /locus_tag="PLB25_00680"
     CDS             4664..4993
                     /locus_tag="PLB25_00680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HOE40143.1"
                     /translation="MTHTVTEACIKCKYTDCVDVCPVDCFREGPNFLTIDPDECIDCA
                     VCIPECPVNAIFAEEDVPKDQRHMTALNAELAKLPSWKSITKRKAPLPDADDWKDKTG
                     KLSLLQR"
     gene            5338..6435
                     /locus_tag="PLB25_00685"
     CDS             5338..6435
                     /locus_tag="PLB25_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOE40144.1"
                     /translation="MSSSQFRAPVPAVIETDALVVGAGPVGLFQVFELGLQGIAAHLV
                     DVLPEPGGQCVALYPDKTIYDIPGVPVCSGRELTHKLLRQIAPFSPVFHFEQQVSTLA
                     QAPDGRWLVTTQAGLQPCQQFLTRTLFIAAGVGAFVPKELKIDGLAAFLGLQLFYQAQ
                     PPSAFAGKQVLIVGGEDAAVANAIALADNGAAQASSVMLVHRRDVLQASSSDLGRFVA
                     LREAGHIQFQAGQITGIDCANRQLRGVLVNTPDDHTLNLPLDVLLVCQGISPKLGPLA
                     QWGLAMQRKQLCVSTETFATSAPGIFAVGDVVTYPGKKKLLVCGFHEATLAAFAAAKL
                     IRPSQSEVLQYTTSSALLRQRLGVAPDTNPP"
     gene            6513..7340
                     /locus_tag="PLB25_00690"
     CDS             6513..7340
                     /locus_tag="PLB25_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proteasome-type protease"
                     /protein_id="HOE40145.1"
                     /translation="MTYCVAIKLNAGLVFLSDSRTNAGLDQISTFRKMIVYEKVNDRF
                     MVLLSAGNLSISQSVREILQVEKLKDNDEDEAITIWNARSMFDAARVLGAAIRHVYER
                     DAASLKLSGVDFNVSLIFGGQIKGEGMRLFQVYSAGNFIEATPETPYFQVGESKYGKP
                     VLDRVIQPETPLDEAAKCALVSMDSTLKSNLSVGLPLDLVVYEVNRFQTDKVVCIDEN
                     NPYFKMMHNSWGARLREVFDSIEDPTWDDGKTDVPLIQPAGRSLPLRKIRTPNEKLI"
     gene            7838..7993
                     /locus_tag="PLB25_00695"
     CDS             7838..7993
                     /locus_tag="PLB25_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40146.1"
                     /translation="MHLQNAAAQHLNFMDPNSCRFDLGNGDLPDTPHPERLQHPFTRV
                     RMPCLSN"
     gene            7978..8196
                     /locus_tag="PLB25_00700"
     CDS             7978..8196
                     /locus_tag="PLB25_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40147.1"
                     /translation="MSVELSFFFFLLALMVAMLGATGSSMHKPPAEGDALYKGPWKWI
                     TSPSDLPQTALIAADAIESPAIAAAGKQ"
     gene            8257..9081
                     /locus_tag="PLB25_00705"
     CDS             8257..9081
                     /locus_tag="PLB25_00705"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOE40148.1"
                     /translation="MHVATIGARHCKGLQAGRFVVRGHWALASTFDVEPHFAILACIQ
                     SRKFSMKKSIGKYSLALTAVSLALLALPAQAVQVSFSGASEANTGDLTGTDRLGNTWF
                     NSNGPLVINSSFTMADNAETPQPFNSENFSNGLGTFANAFQLTVNSSQNQLGFKGIRL
                     QDVTSGLINDFVVKPDVNNSATWIYWETKLSLPDDLGVFQRIRFTAPTGTQLSQGQNF
                     NLNINFEGVITNDTGWAASWDDRLAPEVPGTVPEPTSIALIGVGLLGLAVRRRKQS"
     gene            complement(10106..10312)
                     /locus_tag="PLB25_00710"
     CDS             complement(10106..10312)
                     /locus_tag="PLB25_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40149.1"
                     /translation="MTNKQNSRDKQVPHTGKVDNDTNKLGEKEPTQLNQGKRTPNSRS
                     DREAHVGGSNQSQSRRGPVGTPNR"
     gene            complement(10366..11778)
                     /locus_tag="PLB25_00715"
     CDS             complement(10366..11778)
                     /locus_tag="PLB25_00715"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOE40150.1"
                     /translation="MNPLHVEVAIIGAGTAGMSAYRAARAHADSVLLIEGGVYGTTCA
                     RVGCMPSKLLIAAAEAAHLARHAHAFGVQVQAVAVDGAAVMARVQRERDRFVNSVLET
                     IDAIAPLDRLATKVNFQDANTLLTAHGQLIHARRIVIATGSMPVLPAVVKGLGAHLLT
                     NENIFDLPTLPASLAVFGPGVLGLELAQAMSRLGVRVKVFGVGGGIAGIQDLAIRDYA
                     NQTFNNEFYLDASADVKSVTETAAGVEVNYLHRDGTWRAEPFDYVLAATGRAPVVTGL
                     GLEHTGLQLNDRGVPLFDRFTLQCGDSAIFIAGDASNDSPLLHEAADQGRIAGENAAR
                     FPDVRPGLRRTPLAVVFTDPQVASVGFNLKQLEQHHKGRFAVGLVSFEDQGRSRIMLR
                     NQGLLKVYGEQGTGLFLGAEMFGPAAEHIGHLLAWAAQQRMTVSSMLDMPYYHPVIEE
                     GLRTALRDLNRKLHLEAPVD"
     gene            complement(11806..12540)
                     /locus_tag="PLB25_00720"
     CDS             complement(11806..12540)
                     /locus_tag="PLB25_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOE40151.1"
                     /translation="MLKSREGQKVPDVTFPIRAHGEWHTVDSDTLFKGKTVVLFALPG
                     AFTPTCSNMHLPRYNELASVFKANGIDRLICLSVNDPFVMETWQMAQHTENIDFIPDG
                     NGEFSEGMGLLVDKSAIGLGKRSWRYSMLVKDGLIEKMFIEPEEPGDPFKVSDAETML
                     HYINAKASLPPRVTFFSKPGCEHCARARKMLVDKGLRFEEIELGSHGISYSSLQAVSG
                     RGTTPQVYVDGQHVGGADDLALWLVR"
     gene            complement(12636..13187)
                     /locus_tag="PLB25_00725"
     CDS             complement(12636..13187)
                     /locus_tag="PLB25_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40152.1"
                     /translation="MNTSRNVKASLMAAALLSFSAVHAATLTKAEYKADKTRISAAYK
                     ADKSACDGLKANAQDVCVQEAKAKESVARAELEYSYTGKAADQTKVLEAKAKSAYAVA
                     KEKCDDQAGNAKDVCVKEAKAVETKALADAKMNKQIGEAKKEASEDKSDANYKVAVEK
                     CDAMTGDPKASCVAAAKTKFGKS"
     gene            complement(13506..14375)
                     /locus_tag="PLB25_00730"
     CDS             complement(13506..14375)
                     /locus_tag="PLB25_00730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE40153.1"
                     /translation="MEKKQTSKLPEGSQTAKSLTQVLKASELVRGMVKESADELASVN
                     QMLNEELSNRDRLPDVALALEKSEAVEVKVQEASEKLSRVNQGLENEVKERQLLEQEL
                     GDVIEQQQKALHASLHDALTGLPNRVLFNDRLEHGIEQAKRHGWTLAVMFLDIDKFKT
                     INDTYGHDAGDAVLLTIAKRLTEATRTDDTISRHGGDEFLYLLMEISDEQDATVVANK
                     IIQAVGVPCDVCGAELRVNTSIGIAIFPRDGTTVEVLIKSADAAMYRAKQSRSGYAFA
                     SDNPAAQLERTGQ"
     gene            14501..15238
                     /locus_tag="PLB25_00735"
     CDS             14501..15238
                     /locus_tag="PLB25_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOE40154.1"
                     /translation="MSKPLKSAAPDPKQNQILAALPKSEYERLLPDLELVEMPLGWTM
                     SESGDHVKYLHFPVSGIVSLIYALEDGSSSEIALVGSEGLVGISIYMGGESLPSSTEV
                     QSVGQAYRLSRKIMKHEFEQGGQLQHLALLYTQALICQTSQTAVCNQHHSLEQQFSRW
                     LLMSFDRLHSTKMTITQEQISHLLGVRRESVTQTSGKLQKEGLITKARGSITVVDRAQ
                     LEERTCECYAAVKTECERLLPPPKNAP"
     gene            complement(15453..16454)
                     /locus_tag="PLB25_00740"
     CDS             complement(15453..16454)
                     /locus_tag="PLB25_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HOE40155.1"
                     /translation="MRPAKKPRKPRVVKPKLARPSIAKRVLTVRGPARATKSASTPER
                     ATRLRWTAGIASGVSGIRRYQLFKPSGVQRSERLPLVVMLHGCAQDAKAMAASTNMNR
                     LAQVERFIVLYPEQDRLANLQNCWNWYDTRNGRAQREAESIACAVDHICLTQPVDPRR
                     MAVAGLSAGAGMASLLAVRQPERFQAVVMHSGVGPGLASSPATALGAMRGRRVPVPLM
                     PLVAGLHLPSLLVIQGSADPIVAPGNGAKVAQLWADREGAQAGRPRTVQRGKRYPAKI
                     TDYCNRGRRVATLCLVSGLGHAWSGGEPGLAYSDPNGPDASRMAWSFMKKQFAQRAR"
     gene            complement(16612..17769)
                     /locus_tag="PLB25_00745"
     CDS             complement(16612..17769)
                     /locus_tag="PLB25_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOE40156.1"
                     /translation="MKLATFTTSSATARFSLMALAVIASNCAIADDKAWYLGANVGQA
                     RASIDAAEITSGLLGSGLSTSAIVDENRDLGYKVFGGYQLNKGFALEGGYFDLGQFGF
                     RADTAPAGSLTGNIALRGINLDAVGTLPITERFSAFGRIGAAYTEAKGTFTGTGRVQV
                     LNPNPRNRSTNLKVGLGVQYALSDQWVLRAELERYRVEDAVGNKGDVDLGSIGLVYRF
                     GAKAPMPVSREMAQAPTPVLAAPQPMVVSTPPLAPAPMPPPVKTTFSADSLFDFDKFV
                     VKPAGRVDLDKFAIDLKGLDYDVISVTGHTDRIGSHTYNQKLSERRADAVKSYLVASA
                     GVPANKIRAQGLDGAEPVTKPGDCVGNKATKALIACLQPDRRVDIEVTGKR"
     gene            18518..18748
                     /locus_tag="PLB25_00750"
     CDS             18518..18748
                     /locus_tag="PLB25_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40157.1"
                     /translation="MNYTDPSISRQSLVTAQSLHREAVVHLEKAVKHHRQATLLHDSG
                     DAQQAESHGSMAYNHTAHALEASGRAMNVLLW"
     gene            complement(18781..18986)
                     /locus_tag="PLB25_00755"
                     /pseudo
     CDS             complement(18781..18986)
                     /locus_tag="PLB25_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
BASE COUNT         3947 a         5713 c         5430 g         3896 t
ORIGIN      
        1 taggagtcgc gccctcgcgg cgaatgactt tgaatgtttg aattcttcgc atgacatgca
       61 aagacttgac ttgttagaga ctaacctgat ggtattggac gatgcaacaa actcttaaat
      121 tgatagctgg tggcgcagat ggcacgggcg ctagaggcct ggacttgggg ttttgccaaa
      181 atgtcattga actggcaaac gatgccgatc cgcgcgcgct gaccctcgac ggggtgacgc
      241 ggatcgacct gaagttcccc aaattcaccg atgggcgggc ctacagccag gcgttcttgc
      301 tgcgtcgccg acttggcttc accggcgaac tgcgtgccac cggcgatgtg ctggttgacc
      361 aattgcagca aatgcagcgc agcgggtttg acaccgcggt gttgcgcgcc gaccagaatc
      421 tggccacggc acagcgccaa ctggccctgt tcgaccactt ttaccaaggc gatgcggttc
      481 atccgcagcc gcatttcacc ccggccaccg accaggctgg cgcaccaaag ggcaccctgc
      541 ccagcgcact gcagcccggc tcaaacgccc aacccaccca cggagccagc gcatgaatgc
      601 gatcaccctc aacgcccggc cttcggccac ctttaccgcc aagctgcagg aaaccaccgc
      661 gctgctgcag caagctgcgc tagattacgc cgccaacacg cctggcagcg ccagccgcat
      721 cacgctggcc tgcagcctgg gggccgaaga catggtgctg gccagcctga tcaaccagtt
      781 gcaactcgac attggcatct ttgtgctgga aaccggccag ttgcaccccg aaacgctggc
      841 gctgctggcg cggctgcagg ccagctcgcg cgcaccggtc gaggtgttca ggccggttga
      901 gcatgacgtg ctgcagtttg tcagccaaaa cggcaaggat gccatgtacc agagcatcgc
      961 cctgcgcaag gcctgctgcg gcatccgcaa aatggagccg cttggccgcg cgctggcggg
     1021 caaggccgcc tggatcaccg gcctgcgccg cgagcagtct ggcagccgtg ccgaggtacc
     1081 cctgattgac cgcagcgatg ccagcatcgt caagttcaac ccgctgggcc actggagctg
     1141 gggcgatgta tggcacttta ttgccgtcaa ccaggttgac tacaacccct tgcacgacca
     1201 gttctacccc agcatcggct gcgccccctg cacccgtgcc atcagcgccg gtgaagattt
     1261 ccgcgctggc cgctggtggt gggaagatga agccgccaag gaatgcggct tgcacgtggc
     1321 tggcggtccg ctgcacagcc tgagcgcgca acggtccgac ctggcacagg ttgccgcgtg
     1381 aacgcccgca ccccacacga accactgcaa gccatgagcc atcatcacca ccgccctgac
     1441 catttgagca acgcgcacct ggacgcactc gaagaagaaa ccatattcat cctgcgcgaa
     1501 gtcgccgccg cgtttgagcg ccccaccctg ctgttttccg gcggcaaaga ctctttggtg
     1561 atgctcaaat gcgccgagaa agcctttggc acgcctgcaa ccggcggcaa gattccgtac
     1621 cccttgctga tgatcgacac cggccacaac tttcacgagg tcaccgactt tcgcgatttc
     1681 cgcgccaaag aactcggggc cgagctgatc gtgcgcagtg tcgaggactc gatggcgcgc
     1741 ggtaccgtgc gcttggccca cccaggtgag tcgcgcaatg tgcaccagtc agtcacgctg
     1801 ctcgaagcga ttgatgaatt ccgctttgac gcgctgattg gcggggcccg gcgtgacgag
     1861 gaaaaggcgc gcgccaagga gcgcatcttt tcccaccggg acagctttgg ccagtggcag
     1921 ccgaaagacc agcggcccga gctgtggacg ctgttcaaca ccaaattgca tccgggcgaa
     1981 catttccggg tgttcccgat cagtaactgg accgaactcg acgtgtggca atacatcgcg
     2041 cgcgagcaca tcgccctgcc ctcgctgtat tacacgcacc agcgcgaagt ggtcgagcgc
     2101 aaaggcttgc tggtgccggt aactgaactg acacccttga aaccaggtga ggtggcagag
     2161 ctgcgcgacg tgcgttttcg taccgtgggc gacatcacct gcacctgtcc ggtagaaagc
     2221 ctggccgcaa cgcctgagca aatcgtgctg gaaacgctgg cagccgatgt cagcgaacgc
     2281 ggcgcaacac gcatggacga caagacctct gaggcttcga tggaaaagcg caagaaggat
     2341 gggtactttt gatggctcct gattcagttg acagactggc tgtaaagccc gcgaggcagg
     2401 ccttctgtgt ggcatgcctg tgctggcgta ccagaaatcg tcggccgccc agaatgcctg
     2461 cctcgccaga cgcttttgtg aactgcactt ctgtcaaact tggctcagag tattccacct
     2521 gggttcctca gttgaccgct tgttttcatc ggtaagtcct catgaacctt tacattttta
     2581 acctcgatgg gattcacccg ttgggtgaca gcgctacaca cccgattgaa ccgctgacaa
     2641 cgcgcctggg ggcgttactc ctcgctcgtc cgccaggacg acatgcgggc agaagcggcg
     2701 cgtcgtcgtc gtgcctgccc agccacgccg ccaacggccc actcgggcgc gtccaaccga
     2761 ggaatctggt cacttacaag tcaagtcttt gcaatttccg ggaagaatcc aggaaattca
     2821 tggagtcatt cgtcgcgagg gcgcgactcc tacaaattca aataggcgct gtttgtgtag
     2881 gagtcgcgcg ctcgcaacga aggccgtcca attccggctc gcccgtctta ggttcaaagg
     2941 agcattgatc aacggccatc tcagcgcagc agtcggattg ggcggccccc gatttctggt
     3001 acgccagcat gagggggccg cccgatccgg ctgctgcgca gcccccaccc gagacaaaaa
     3061 aataacccgc atttccatct gctcaccgaa caaccctcat gaatactaca actaatatag
     3121 ctactcacgc agacagcact agcgctacag cccaaaatga tcatcaatcg gcgctcaagt
     3181 tcatcacctg cggcagtgtt gatgacggca agagcacgct gattggtcgg ctgctgatcg
     3241 acagccgatc agtgctgcaa gaccagttgg ccaatgtgtc aaaaagcggc gaggccgacc
     3301 tggcccagtt caccgatggc ctgagcgccg agcgcgaaca aggcatcacc atcgatgtgg
     3361 cttaccgcta cttcaacacc gcacagcgca aattcatcat tggcgacgca ccagggcacg
     3421 agcaatacac ccgcaacatg gtgactgccg ccagcagcgc cgatgcggcg gtggtgctgg
     3481 tggatgccac caagctgcag tggcaagacc cggcgctcga actgctgccg caaacacgcc
     3541 gccatgcgct gctggtgaac ctgctgcgcg tgcccagcat cgtgtttgcc atcaacaagc
     3601 tcgacgcgct ggaagatggc gccacgctgg cgttcaccaa catccgcgcc gcgctggaag
     3661 aatttgccag cgcagcgggc attgaaattg ccaccgtggt gccaatttct gccctgaaag
     3721 gccacaacgt cgtcgataca gtggacggct ggtgcggcta caccggcccg agcctgctcg
     3781 acattctgga gctgctgccc aacacgccag ccgacagcgc gctgccactg gccctgccgg
     3841 tgcaatgggt ggaaaaattc tcttcgagca gcgacacccg ccagggccga cgcatcttct
     3901 ggggccgggt tgccagcggc ctggcaatgg tcggccagat ggtcaaggtg ctacccagcg
     3961 ggcaaaccgc cacggtagcc caggtgctgg gtcacacccg ccagcctggc acggtgcaag
     4021 cgggtcacag cgctggcatt gtgcttgacc gtgaagttga tgtgtcgcgc ggcgactggc
     4081 tgctggcctt tgcggcaagc gctgcagcgg ccacagcgga cgatgatttt gacaccccca
     4141 gcgcccccac cagcctgcca ccgagtcgcg aactggcggt gaccgtggcc tggatggatg
     4201 acgaaccgct ggttgcgggc agagtctatt gggcgctgca cggccaccgc tgggtcaagg
     4261 cgcgcgtcag gcgcgtggtg catcggctcg acatcaacac cctggccgaa gacgatgccg
     4321 aggcactgcc ggccaacgcc atcggccatg tcgaactgct actgcaagag ccaatagcag
     4381 cgcgcccgtt tgcgcagtcg cgcgtgctgg gttcgctggt gttggtggac accgcttcgc
     4441 accgaaccgc tggcgcggcg ctggtcaatt agcgatggca cggcaaattg acggagccga
     4501 catcaagcca gcagccagtc agatttcggc acgcggcatc gttgcaatcg caccgccctg
     4561 gggcaaacag cagcgttatt gccggcccca taccatgctc accaacttgg ggcgggaaac
     4621 ccaataaaat cctgcttttg cgtccaactg tagaaatttc accatgaccc ataccgtcac
     4681 cgaagcctgt atcaaatgca aatacaccga ctgcgtcgat gtctgcccag tcgattgctt
     4741 tcgcgaaggc cccaactttt tgaccattga cccagatgag tgcattgatt gcgcggtgtg
     4801 cattccagag tgtccggtca acgcgatttt tgccgaagag gacgtaccca aagatcagcg
     4861 ccacatgact gcgctcaacg ccgaactggc caaactgcca agctggaaga gcatcaccaa
     4921 gcgcaaggcc ccgctgcctg acgctgacga ctggaaagac aagaccggca agctgtcgct
     4981 gttgcagcgt taagggatgc gcagactgcg aacctgccat tcatggcgag tgttgacggg
     5041 atgcagctta gtctctaaca agtcaagtct ttgcatatca tgcgaagaat tcaaaccttc
     5101 aaagtcattc gtcgctacaa attcagatgg atgccgcctg tttgtgtagg agtcgcgccc
     5161 tcgcgacgaa ggccgtgcaa ttccggctcg tccggtctag gcttaaggct cgcggtgtgg
     5221 caagccaagg gcttgtccac aaatgacatg tacagcttat tcatggacca gccctgaacg
     5281 agcggattgc aaccacggca tgtgcccgtc agtgccgcca gaaatgggat agttgtgatg
     5341 tcctcatccc aatttcgcgc cccggtgcct gccgtgattg aaaccgatgc gctggtggtc
     5401 ggtgccggac cggtcgggct gtttcaggtc tttgagctgg gtttgcaagg cattgcagcc
     5461 catctggtcg atgtactgcc cgagcctggc ggtcagtgcg tggcgcttta ccctgacaaa
     5521 accatttacg acatccccgg tgtaccggtt tgcagtgggc gcgagctgac acacaagctg
     5581 ctgcgccaga tcgcgccttt ctcgccggtg tttcattttg agcagcaggt cagcacgctg
     5641 gcgcaggcac cagacgggcg ctggctggtc accactcagg ccggcctgca gccttgccag
     5701 cagtttctga cgcgcaccct gttcatagca gccggtgtcg gggcctttgt gccgaaagaa
     5761 ttgaagattg acgggctggc ggccttcttg gggctgcaac tgttttatca ggcgcagccg
     5821 ccgtctgcct ttgctggcaa acaggtgctg attgtgggcg gtgaggatgc tgccgtggcc
     5881 aacgccatcg cactggccga caacggtgcg gcgcaagcca gcagtgtcat gctggtgcac
     5941 cgccgcgatg tgctgcaggc aagcagcagc gacctgggtc gctttgtggc actgcgcgag
     6001 gccgggcaca ttcagtttca ggcgggtcag atcaccggca tcgactgcgc caatcggcaa
     6061 ttgcgcggcg tgctggtcaa caccccggat gaccacacgc tgaacttgcc actggatgtg
     6121 ctgctggttt gccaggggat cagcccgaag ctcgggccgc tggcgcagtg gggtctggca
     6181 atgcagcgca agcagctttg tgtcagcacc gagactttcg ccaccagcgc gcccggcatt
     6241 tttgcggttg gcgatgtggt gacctatccg ggcaagaaaa agctgctggt ctgcggcttt
     6301 cacgaggcca ctctggcagc ctttgcggcg gcaaaactga ttcgcccgag ccagtcggag
     6361 gtgctgcaat acaccaccag cagcgcattg ttgcgccagc ggctcggtgt agcgcccgat
     6421 accaacccgc cctgatgtcg cgccaatcgt ccgacaggtg atgccatcgg gcggtctaca
     6481 ctctcccctt ttgttcttgt cgctccaaac acatgaccta ctgcgtcgcc atcaaactca
     6541 atgccggact ggtttttctg tccgattccc gaaccaacgc cggtcttgac cagatcagca
     6601 cttttcgcaa aatgatcgtt tacgaaaagg tcaatgaccg tttcatggtg ctgctgtcgg
     6661 ctggcaacct gagcatttca cagtcggtgc gcgagatttt gcaagtcgaa aaactcaagg
     6721 acaacgacga ggacgaagcc atcacgatct ggaacgccag gagcatgttt gacgcggcca
     6781 gggtgctcgg tgccgccatc cgccatgtgt acgagcgtga tgccgcgtcg ctcaagctgt
     6841 ccggcgttga cttcaatgtg tcgcttattt ttggtggcca gatcaagggc gagggcatgc
     6901 gattgtttca ggtctattcg gctggcaact tcattgaggc cacgccagag accccttact
     6961 ttcaggtggg cgaatcaaaa tacggcaagc cggtgcttga tcgggtgatt cagccagaga
     7021 ccccgctcga tgaggccgcc aagtgtgcgc tggtgtcgat ggactcgacg ctcaagtcca
     7081 acctgtcggt cgggctgccg ctggacctgg tggtctatga ggtcaaccgc tttcagaccg
     7141 acaaagtggt ctgcattgac gagaacaacc cctacttcaa gatgatgcac aacagttggg
     7201 gggccaggct gcgcgaggtg tttgacagca ttgaagaccc aacctgggac gatggcaaga
     7261 ccgatgtgcc gctgatccag cccgccgggc gcagtctgcc cctgagaaaa atccgcacac
     7321 cgaatgaaaa gctgatctga atttctcttg cgcccagcgc aggcagacca gtccagcggc
     7381 cccgatgcgc gccgttgaca agtttggccg gtggcccgga ctgcccggtc acctgcaact
     7441 ggttgcctct ggtgccgcaa cggcatggct ttgtgcaagc agcagcgacg attgccgacg
     7501 catcggcctg actgattggc tattggctgg atggttttcg cgcgcctgag gccgcttgac
     7561 ccaggaagca ccgcttgatc tggatctgga tcttgactct accggcgagc acctcaagtc
     7621 gcccgatcat caacgtgaac aattcggatt cgcttgaccg ggtcgcgtca tgtgggttgg
     7681 ccatgtgccc ggttgggtaa ggtgtgctcg ataagtgacc tgagacacag accgccagca
     7741 gcctggtgcc ttatggtgtc gggcttgggt caagcgttct gcccggcttt tccaaagcgg
     7801 cgtgaactga ctcacaacac tacccaaagg accctccatg catttgcaaa atgccgcagc
     7861 ccagcacctg aactttatgg accccaattc ttgccgcttt gatctgggca acggcgacct
     7921 gcccgacaca ccacacccag aacgcctgca acatcccttc accagagtgc gcatgccatg
     7981 tctgtcgaac tgagcttctt cttttttttg ctcgccctga tggtcgccat gctgggcgca
     8041 acaggctctt ccatgcacaa gccaccggca gagggtgatg ccctctacaa aggaccgtgg
     8101 aagtggatca ccagcccatc cgacttgcca caaaccgcgc tgattgccgc tgatgccatc
     8161 gagtcgcctg ccatcgctgc tgcgggcaag caatgactct ttgaccagcc atccgaaaaa
     8221 aaagcgcaag ggttcgccac cacactgacc acgccaatgc atgttgcgac catcggagcg
     8281 cgtcattgca aaggcttaca agctggtcgc ttcgtcgtac gcgggcactg ggcactcgca
     8341 tcgacattcg atgtcgagcc gcactttgca attcttgctt gcatccaatc tagaaagttc
     8401 agtatgaaaa aatcaatcgg gaagtattcc ctggccctga ccgctgtcag cttggctttg
     8461 ctggccttgc cagcacaagc cgttcaagtc agcttttccg gcgccagcga ggcgaacaca
     8521 ggtgacctaa ctggcactga ccgcctgggg aacacttggt tcaacagcaa tgggccgctt
     8581 gtgatcaaca gcagctttac catggcggat aacgctgaaa ctccgcagcc gttcaatagc
     8641 gagaacttca gcaacggttt gggcacgttt gccaatgcgt ttcaactgac cgtcaacagc
     8701 agccagaatc agctgggctt caaaggcatt cggctacagg atgtgacttc tggtttgatc
     8761 aatgactttg tcgtcaagcc agacgtcaac aactcagcca cctggattta ttgggaaacc
     8821 aagttaagcc tgccagacga tttgggcgtt ttccagcgaa tccggtttac agcgcccacc
     8881 ggcacacagc tttctcaagg acagaacttc aatctcaaca tcaacttcga gggcgtcatc
     8941 accaacgaca caggctgggc tgcatcctgg gacgaccgct tggccccgga agtgcccggt
     9001 accgtgccgg agccgacctc tatagcactc atcggcgtgg gcctgctcgg cctggcggtg
     9061 cgtcggcgca agcagtcctg agcggcgggg agtcaatcaa cgctgctgtc agcaacgcct
     9121 ggggctgaca ttcagccatc aaaacgcgac ccatgacttg acgcgtgttt ctacccaaaa
     9181 aaagccgccc ggctggtccg gcggcttttc tcattgcgct ttctccaact caaacacaac
     9241 ggctacgtcg caccctgtca ggaccacgca gacgccatct ggacggtgac ctgaggaccg
     9301 aggtgtccca aagcctgggt gcctgccgga cttatggcgc attggctccg aacggaacga
     9361 agcggccctc tttgcacccg cgctttgacc cacaactcgg caatggcctg gcgcgactgc
     9421 gatcaccatg ggagacactg gcgcgatctg acagcgagcc agaatgccca aatgccgttc
     9481 catcgaactt gaaagcagcc ctaggtgttt accaccatca gcgcgggtgc ttctgtggct
     9541 gtcatgcccg acttgccgcc acgctcgtgc gttgccggtt gcagcatgcc aggcgactca
     9601 tgctcttatc gttagtgctt ggcaaatgcc acaatcaaaa atatatgata aatcagccca
     9661 tagcacatac tggctgtgcg ctggaagcta caaacagtat agcaatttgt catgaagtca
     9721 ggtcgcctgg cggacgcatc gacagcagtt caggggtcaa gcaggaataa cttggacata
     9781 gggggtcgct atggcgagtg ccgggccagg cgcaatctgc gcggcgcaat cggctgcaca
     9841 agcggttcgc aacaacgcat ggcgcttgtc agtgcggccg aactgttcaa gtgatttttg
     9901 cctgacacct tagtgaaggt cttcctgctg gccccgccat tggaggccaa ggcaagaccg
     9961 ccagcatcgg caggccaagc atggcgtttt gcttaagggg gtaagggcag tggaaaaaat
    10021 tgaggtgcgt ctgcaggcag gcgcggccag cttacccgac ccgccccgct tcagccatgc
    10081 gcgttagcga acaaggtccc gccaatcatc gattgggggt gcccaccggc ccgcgccgcg
    10141 actgcgactg attgctgccg cccacgtgcg cctcgcggtc gctccggctg tttggggtac
    10201 gcttgccctg attcaactgt gtgggctcct tctcacccag cttattggtg tcgttatcga
    10261 ccttgccggt atgaggtacc tgtttgtcgc gtgagttttg tttgttggtc atcttgattt
    10321 cctggtgaaa tggactgttg ccattgaatt cagagctgaa cagcgctagt caacgggcgc
    10381 ttccaggtgc agcttgcgat tgaggtcgcg cagggcagtg cgcagcccct cttctatgac
    10441 cggatggtag taaggcatgt ccaacatgct tgagaccgtc atgcgttgtt gcgccgccca
    10501 ggccagcaga tgcccgatgt gctcggcggc agggccgaac atttctgccc ccagaaacag
    10561 gccggtgccc tgttcgccat aaactttcag cagaccctgg ttgcgcagca tgatccgact
    10621 gcgtccctgg tcctcaaagg acaccagtcc gacggcaaag cggcccttat ggtgctgttc
    10681 aagctgtttc agattgaagc caaccgaggc cacttgcggg tcggtgaaaa ccaccgccag
    10741 cggcgtgcgg cgcagtccgg gccggacgtc gggaaagcgc gcggcgttct cgcccgcaat
    10801 gcgaccctgg tctgcggctt cgtgcagcag ggggctgtcg ttgctggcat cgcccgcgat
    10861 gaaaattgcg ctgtcgccgc actgcaaagt gaagcggtca aacagtggaa cgccgcgatc
    10921 attcaactgc aagccggtgt gttccaggcc aagaccagtc acaacaggtg cccgaccagt
    10981 tgctgccagc acataatcga acggctcggc tcgccaggtg ccatcccgat gcagatagtt
    11041 gacttcgacc ccggcggctg tttctgtgac tgacttcacg tcagccgaag cgtccagata
    11101 gaactcgttg ttgaaggtct ggttggcata gtcccgaatg gccagatcct gaatgccggc
    11161 aatacctccg cccacaccaa acaccttgac acgcacaccc aggcggctca tggcttgggc
    11221 cagttccagc cccagcacgc caggaccaaa caccgccagg ctggccggca aggtcggcaa
    11281 gtcaaagatg ttctcattgg tcagcaaatg cgcgcccagc cctttcacga ccgcaggcaa
    11341 aacaggcatg ctgccggtag cgatgacaat gcgcctggcg tgaatcaact ggccgtgcgc
    11401 tgtcagcaag gtgttggcat cctggaaatt gaccttggtg gctaatctgt caagtggcgc
    11461 aattgcgtca atcgtctcca gcacagagtt gacaaaacgg tcgcgttcgc gctgaacgcg
    11521 cgccatcacg gcggccccgt caaccgcaac cgcttgcacc tgcaccccga aggcgtgcgc
    11581 atggcgtgcc aaatgcgcgg cctcggcggc ggcaatcagc aatttgctgg gcatgcaacc
    11641 cacgcgcgcg caggtagtgc catagacacc cccttcgatc agcaaaaccg aatcagcgtg
    11701 ggcacgggca gcccggtagg cactcatgcc tgccgtgccg gcaccaataa tggcgacttc
    11761 gacgtgaagc gggttcatgg cggtctgcag cggtggcgtg gcaatctacc tgaccagcca
    11821 gagcgccaaa tcatcggcac cgccaacatg ttgaccatcg acataaacct gcggcgtggt
    11881 gccgcgtccg ctcaccgctt gcagtgagct gtagctgatg ccatggctgc caagctcgat
    11941 ctcttcgaaa cgaagtccct tgtcaacgag cattttccgg gcgcgcgcgc agtgctcgca
    12001 accgggcttg ctgaagaaag tgacacgcgg cggcaagctg gctttggcgt tgatgtagtg
    12061 cagcatggtt tcggcatccg acaccttgaa tggatcaccc ggttcctcgg gctcgatgaa
    12121 catcttttca atcaggccat ctttcaccag catggaataa cgccaactgc gcttgcccag
    12181 accaatcgcc gatttatcca ccagcaggcc cataccctca gaaaactcac cgttgccatc
    12241 agggatgaag tcgatgtttt cagtgtgctg cgccatttgc catgtttcca tcacaaacgg
    12301 gtcattgacc gacaggcaaa tcaggcgatc aatgccattt gccttgaaca cactggccaa
    12361 ctcgttatat cgaggtaaat gcatgttgga gcaagttggc gtgaaggcac ccggcagcgc
    12421 gaacagcacg acggttttgc ctttgaacag ggtgtcgctg tcgaccgtgt gccattcgcc
    12481 atgagcccgg atcgggaacg tgacatccgg gactttttga ccttcgcgtg atttcagcat
    12541 ggatttcctt ggattcgttt ggtgccggca ttgctttggc cgtgctatcg gttgcaagct
    12601 gtcttgccag caccaggaac tatttttgag atggttcagg acttgccaaa cttggttttg
    12661 gcagccgcca cacaactggc tttgggatca cctgtcatgg cgtcgcactt ctcaacagct
    12721 accttgtagt tggcatcgga cttgtcctcg gaggcctctt ttttggcctc gccgatttgt
    12781 ttgttcatct tcgcatccgc cagagccttg gtctctactg ccttggcttc cttgacgcag
    12841 acatccttgg catttcctgc ctggtcatcg catttttcct tggcgacggc gtaggcagac
    12901 ttggccttgg cttcaagcac cttggtctgg tcggcggctt tgccggtgta gctgtattcc
    12961 agctctgcgc gggctaccga ttccttggcc ttggcttctt gcacgcagac atcctgggca
    13021 ttggctttca aaccatcgca agcgcttttg tcagctttgt aggcggcgct tatgcgcgtc
    13081 ttgtcggcct tgtattccgc tttggtgagt gtcgcagcgt gaaccgctga aaatgacagg
    13141 agggcggcgg ccatcagaga ggctttgaca ttgcgggagg tgttcatagg tgtccttttt
    13201 tgtaaatgtc aaccatcagg aagcagcgac agatgccgcc actgaagcac ggaccaaatg
    13261 atcatgcgat ggttgacagg caccaactct aaggaaacgc aggcgagttg tctgtgcgct
    13321 ggcaaaccaa cgaaaatctg cgccacagca caatcattga caggtttcgc ttctgggacg
    13381 gtgggtatcc atcggttgac ttttgacacg gcgttctccc ctcgtcccac agatacccgt
    13441 gccacacacg aggctggctt tgccaggcgg acctgtcaac aaattggaaa tgacttgggc
    13501 atgaactatt gcccagttcg ctccaactgc gccgcagggt tgtcacttgc aaaggcatag
    13561 ccggatcgac tttgcttggc ccgatacatg gcagcatcgg cactcttgat caagacctct
    13621 accgtggtgc cgtctcgggg aaatatcgcg atgccaatgc tcgtgttgac cctgagttca
    13681 gcgccgcaaa catcacacgg caccccaacg gcctgaatga tcttatttgc gacgacagtt
    13741 gcatcttgct catcacttat ctccatgagc aaatacaaaa attcatcgcc gccatgccgg
    13801 ctgatcgtat cgtcggtgcg cgttgcttct gtgagtcgct tggcaatcgt cagcaaaaca
    13861 gcgtcgcctg cgtcgtggcc gtatgtatcg ttgatcgttt tgaacttatc aatgtccagg
    13921 aacatcacgg ccagtgtcca gccgtgccgt ttggcttgct ctattccatg ttccagacgg
    13981 tcgttgaaaa gcacgcgatt tggcaagccg gttaacgcat cgtgcaaaga ggcatgaaga
    14041 gccttctgtt gctgctcgat gacatcgccg agttcctgct ccaggagctg tcgctccttc
    14101 acctcgtttt ccagaccttg attcacccgt gacaactttt ccgacgcctc ttgcactttg
    14161 acttcaacgg cttcactttt ttcaagggcc aacgccacat cgggcagccg gtcccggttc
    14221 gacagctctt cattgagcat ctggttgacc gacgcaagct catcggcaga ctctttgacc
    14281 atgcctctga cgagttcgct ggcctttaac acctgagtca aagacttggc tgtctgagag
    14341 ccttcaggca gtttggatgt ctgtttcttt tccatggaga ggcgcagttg aatagttcga
    14401 tacgtcgctt tacgggacta ccctgccaat aatggcagca gcagaggaaa tggtctgtgc
    14461 gatggcttac atttgacccc catagtttca caaagtattc atgtccaaac ctttaaagtc
    14521 cgctgcgccc gacccgaagc aaaaccagat tcttgctgcg cttccaaaat cggagtacga
    14581 gcgactgttg cctgatctcg agctagttga aatgccgcta gggtggacga tgtcggaatc
    14641 gggcgaccac gtgaaatatc tgcactttcc ggtctcgggc atcgtctcgc tgatctacgc
    14701 cctcgaagac gggtcttcaa gtgaaatcgc cctggtcgga agcgaaggtt tggtcggcat
    14761 ttcgatttac atggggggtg aaagcttgcc aagcagcacg gaggtgcaaa gcgtcggcca
    14821 ggcctatcga ctcagccgca agatcatgaa gcacgagttc gagcagggcg gtcaactgca
    14881 acacctggca ttgctttaca cccaggcatt gatttgccag acctcgcaga cggcggtctg
    14941 caatcagcat cattcgctag aacagcaatt cagccggtgg cttttgatga gttttgaccg
    15001 cttgcacagc acaaagatga cgattaccca agagcagatt tcgcacctgt tgggggttcg
    15061 ccgtgagagc gtgactcaaa cgtcgggcaa gttgcaaaag gagggattga tcaccaaggc
    15121 ccgtggcagc atcaccgtgg tcgatcgagc ccaacttgaa gagcggacat gcgaatgtta
    15181 tgccgcggtc aaaacagagt gtgaacgtct gctcccgccg cccaaaaacg caccttgaac
    15241 aagggctcac caattcgtca atatcaggca cagacccaaa gcgacacatt gctgagttga
    15301 actggtgtgc caaaccactc cctgggcaga cgttgcgaca gtcacatgtg gcaagcagta
    15361 gtggagaact tggctctgtt gcccctgaag cgaaggccaa tcacaaaccg ccttcaccgc
    15421 cgggctcgat atcgacaagg gtggctgaac gcttacctgg cgcgttgggc gaattgcttt
    15481 ttcatgaaag accaagccat gcgtgaggca tccggaccat tcgggtcgct gtaggctagg
    15541 cccggctctc cgccactcca ggcgtggccc aatccgctga ccaggcaaag cgtcgctaca
    15601 cgcctgcccc ggttacagta atccgtgatc tttgcagggt agcgtttgcc acgttgcacc
    15661 gtgcgtggcc gacccgcttg cgcgccctcg cgatcagccc acagttgggc aacctttgcg
    15721 ccgttaccgg gtgccacgat cggatcggcg ctgccttgaa tgaccagcag cgagggcaga
    15781 tgcaggccag caaccagcgg catgagcggc acaggcactc tgcgtccgcg catagccccc
    15841 agcgcggtag ccggggaact ggccaggcct ggcccgacgc cggaatgcat caccactgcc
    15901 tgaaagcgct cgggctgacg aactgcaagc aagctcgcca taccagctcc ggccgaaaga
    15961 cccgccacgg ccatccgccg cgggtccacc ggttgcgtca gacaaatatg gtcaacggcg
    16021 caagctatcg actcggcttc tcgctgcgct cgaccatttc gcgtgtcgta ccaattccag
    16081 cagttttgca gattggcaag tcgatcctgt tccggataca gaacgataaa gcgttcgacc
    16141 tgagccaggc ggttcatgtt ggtactggct gccatcgcct ttgcatcctg tgcacagcca
    16201 tgcagcatca ccaccaatgg caggcgctca ctacgctgta cgcccgacgg cttgaaaagc
    16261 tggtagcgcc ggataccact gacaccggac gcaatacccg cggtccaccg aagccgcgtc
    16321 gcgcgctccg gtgtacttgc agattttgtg gcgcgcgctg ggccacgtac tgtcaaaaca
    16381 cgctttgcga tgctgggtcg agccagcttg ggcttgacaa cgcggggttt gcggggtttt
    16441 tttgctggcc tcatggccag ctttatggcc ttggcactgg tgcgcaatgc caatttcgcc
    16501 aaggcgttga atgatcgctg gagtgatcgg ctcaagatgt cagcctatcg atgccctgaa
    16561 accgaagcgg gccggtacca agagcgatca gtcttcaaga catggtcttt tctagcgttt
    16621 gcctgtgact tcaatatcaa cacgacgatc aggctgcagg cacgcaatca gggccttggt
    16681 cgctttgttg ccgacgcagt cccccggttt ggtcactggt tcggccccgt ccaggccctg
    16741 ggcgcggatt ttgttggctg gcacaccggc agacgcgacc aagtagcttt tgaccgcgtc
    16801 agccctgcgc tcggacaatt tctggttata ggtatgcgag ccaatgcggt cggtgtggcc
    16861 agtcacgctg attacgtcgt agtccaggcc tttgaggtcg atggcgaact tgtccaggtc
    16921 aacccgtccg gcaggcttga ccacgaactt gtcaaagtca aacagggagt cggccgaaaa
    16981 cgtggttttg actggcggtg gcatcggcgc tggtgccaac ggtggggttg aaacaaccat
    17041 gggttgcggc gcggcgagca ccggtgtggg tgcctgggcc atttcccgcg atacgggcat
    17101 gggtgccttg gctccaaatc ggtagaccag accaatcgaa cccaggtcaa cgtcgccctt
    17161 attgccaacg gcatcttcga cgcggtagcg ctccaactcg gcacgcagca cccattggtc
    17221 gctcaaggca tattgcacac caaggcccac cttgagattg gtgctgcggt tgcgcggatt
    17281 ggggttgagc acctgtacac gaccagtgcc ggtaaacgtg cctttggctt cggtgtaggc
    17341 cgcgccaatc cgaccaaatg cagaaaaccg ctcggtaatg ggcaaggtgc ccaccgcatc
    17401 aaggttgatg ccgcgcaacg cgatattgcc ggtcaggcta ccggcaggtg cggtgtcggc
    17461 gcgaaagcca aactgcccca gatcgaaata accgccctcc aatgcaaagc ccttgttgag
    17521 ctgatagccg ccaaatacct tatagcccaa gtcacggttc tcatcgacga tggcgctggt
    17581 gctcaggcca ctgcccagca gcccgctggt gatttcggcg gcgtcgattg atgcccgcgc
    17641 ctgcccgaca ttggcaccga ggtaccaagc tttgtcgtcg gctatggcgc agttgctggc
    17701 gatgaccgcg agggccatca aactgaatct ggccgtagcc gaagatgtcg tgaatgttgc
    17761 gagtttcata tttttctcca agaaaatgag cccgccttga tgcgatgcag gggcgggcga
    17821 agggtggtta cgaaaaatct tttttgaggc cggtcacgtt gatgacggtg tccaccagcg
    17881 tgaccgatgc gcccagagac aaagcccgcc cgttcagggt gacacccgat tgaaccgctg
    17941 ccaatacctc tgggggcgtt agtcctcctg gcgggcagca gatcacctgg tcgtaccttc
    18001 acagccgctc cgccagcggc ccaccagggc gtgctcaact gaggaatctg ggttcaatgc
    18061 catgcgtctg ataggcagct atcgcaaccc gaaaaacgac aagaccgcga gaacaatgac
    18121 gaccgcgccg accaagtaaa tgatgctgtt cacaattgac ctctttagtt gaagttgaga
    18181 gaaatgcagg atctgagttt ctcgacccaa tcgactcaga cctaccgggt acccattgca
    18241 ccgctcgcag gccgggccgt ctgtatgcca gcgaacacag aacacaaaga ctggttccct
    18301 ttccagatca ttcgtttcaa ggtatcgcgc cacacacatt gcaagcgcaa ggcaaacata
    18361 gatggcaacg agatggatgg tttggaagcg gaatcaagcg cccgcaaacg aacctggcgc
    18421 tcaacaggtg cgggcaaggt gtcactttgt acgcttgcgc accgacggct gcccgaggaa
    18481 tgaatagcat ttgctggcat ccaaatcagg taacgccatg aactacacag acccatcaat
    18541 ttcccggcaa tccctcgtca ctgcgcagtc actgcatcgt gaggcggttg ttcacctgga
    18601 aaaggccgtc aagcatcacc ggcaagcgac attgcttcac gactctggtg atgcacagca
    18661 ggccgagtcc catggaagca tggcctacaa ccacactgcg cacgcgctgg aagcgagtgg
    18721 ccgtgcaatg aacgtcttgc tctggtaggg cgtctatgct ggcttgcgag ccgcgcgggc
    18781 ggccagtttg cgccgttcac gcagcaaccg gcgcaagctg gccttgagca gcgcgctgtc
    18841 gacattggtc tgcatcgaca cctgcaatgt gttcaccttg cgattgccgg tgacgcggcg
    18901 ctgcagggtg tgcagcggca acagcacggt gtcgtcctgg tcgcccatgc cgccctggcc
    18961 cttggccgcc aggatgccca ccacgg
//
LOCUS       DAOSLL010000009        18715 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_3192_length_18715_cov_5.058521, whole genome shotgun sequence.
ACCESSION   DAOSLL010000009 DAOSLL010000000
VERSION     DAOSLL010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 18715)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18715)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18715
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3192_length_18715_cov_5.058521"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..1064
                     /locus_tag="PLB25_00760"
     CDS             147..1064
                     /locus_tag="PLB25_00760"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HOE40158.1"
                     /translation="MTTSQATVHTSSLTSLTLLARGKVRDNYAVGDDRILMVASDRIS
                     AFDVIMGQPIPGKGVLLTQMALFWFDKLGPKGLNLCPIHLTGEAPESVVTPAEVPQVA
                     GRSMLVKRLRPIPVEAVVRGYLAGSGWKEYQQGRSVCGVPLPEGLQNASKLPAPIYTP
                     AAKAAMGEHDENISFERTVEMIGADLAGRIRSISIAIYQAASQIAAAKGIIIADTKFE
                     FGLDQDGTLVLMDEVLTPDSSRYWPAESYVEGDNPPSYDKQFLRDWLETAQVAGKPWS
                     KTPPAPHLPREVIEKTAAKYQEAMARLTG"
     gene            1841..2173
                     /locus_tag="PLB25_00765"
     CDS             1841..2173
                     /locus_tag="PLB25_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40159.1"
                     /translation="MSPIKAKAANAHKDLQMKTSGQPRNLGAMFLANFTDVDKARSIA
                     NRRWLRPAPVVKANGRSALALAPKLTDLTGIFHWPGRLVALQPKGLPGGAGSVVNGLS
                     AASGAWLK"
     gene            2170..3015
                     /locus_tag="PLB25_00770"
     CDS             2170..3015
                     /locus_tag="PLB25_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40160.1"
                     /translation="MSRLRIKSLRFLLGFHLPVVAIALLTGLLSLNSFADLPEGHLRA
                     SPQPEHDTQRIAAIAGINQQMGRSQIEVGSMLQKAASGQANEAQMYRFHAAVLRRLSV
                     LQQAMAGIADEVAYNLPLMQAGRNFGNDKSNVIMATSLAAVDPDSAMRHALAAMQGLV
                     MMAEQSPSIANTIAVEMAERSKAHAQSFEQHSVQTGLTGIVVMALLLLWLYVSQRVTR
                     RLSRLVPALRGFALDDIEPRLLAQVCQLSGQQNRCCVTWRCRCWLFGKASLQERRRNV
                     IWGNV"
     gene            2925..6971
                     /locus_tag="PLB25_00775"
     CDS             2925..6971
                     /locus_tag="PLB25_00775"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE40161.1"
                     /translation="MLCNLALSLLAFRESVIARKTAQCDLGERMNVLSCLYDVMHLTE
                     DPGRDQDEMLEAVLQRLPGAMRYPDIAAGWIDFSGRRYGSSASGEQLSVCFGGTPKQP
                     DLLGVTYIAPLPTDAREPFLAQERSLFEALGKRLTNVMQRRRTERSLQQADRALRTAR
                     QCGQLLNRAEHEDQLMQDTCRLAVEVGGYRMAWVGLAENDEARSVRPVARAGFDDNYL
                     DAAQISWADGARGRETTGTAIRERRTVVTQDILTNPDLAPWREAAMRCGYGAAIAMPL
                     LAEADQCIGALCLHAGASDAFSDAEVQHLVEMASDLSYGIRTLRTRSALNSSLVELRK
                     LSLAVAQSPNAIMVTSMEPRIEYVNEAFTRITGFTREEVLGQNPRLLQSGKTPATTYQ
                     DMWQTLLGAKIWTGEFINHTRQKEELIEAAIIVPLVESDGQVSHYVAIKEDITDKKRM
                     RDELDRHRLHLEELVALRTGQLEAALQQQVALFEAASVGIAVLRERIIVRCNRTLDEM
                     MDYVPGEQIGQSVRIWYPDQSAYAKASQELYDRPNCGEVRTVERDLVRKDGSHFWARL
                     SVRSIDPTGLSKDMVCIVEDLTQERAVIAEISQARLQAESANRSKYDFLANMSHEIRT
                     PMNAIIGMSHLALQTDLDKKQRNYIEKVHRSGQSLLGIINDILDFSRLETGKMSMETA
                     AFNLEDVMDNLANLVGLKTEEKGLELLFHTAPDVPTALIGDSLRLGQVLINLSNNAVK
                     FTEKGEIVIGIDKVADHEDGVELHFRVRDTGIGMTPEQCGKMFHGFGQADTSTTRKYG
                     GTGLGLAICKNLVEQMHGKIWVESVAGVGSTLHFHVRLGVQAQAQVWRMFKAEQLQGV
                     RALVVDDNAAAREILSAMVTTFGLQVDVAQDGAQALHLIAAADKKAQPYELVLMDWKM
                     PVMDGLAAAHHLHSEQLSRVPTVIMVTAHGREDVMASAREHGVAVQSVLTKPVTPSVL
                     LKAIGEALGKDTNVTTREEARADDNARAIEKLKGARVLLVEDNEINQELAMELLTSSG
                     MTVVLANHGQEALDILRQDRHFDAVLMDCQMPVMDGYTATREIRRNPVFKDLPIIAMT
                     ANAMAGDKEKVLQVGMCDHLAKPIDVAAMFATLAKWIDPSATVADASLAIKKEAVSAI
                     ETGATGIFDQLSRAGIDTRSGLAAAINKEPLYRRLLLKFRDGQGAFADLFAAARSGSD
                     STAAQRCAHTLRGTAATIGARGVQEAAAKLEQACQQQAADAQIDDLLRLVLEELQPVL
                     AALQTFGSDVAGVPVHLPVAVDAVKLSGVRASLMELLDLGDARAIDLFDAHEALFRVA
                     YPAQWKTIASSVHDFDFESALALIQQSS"
     gene            6999..8213
                     /locus_tag="PLB25_00780"
     CDS             6999..8213
                     /locus_tag="PLB25_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HOE40162.1"
                     /translation="MDHAEEGIVKATILVVDDTPDNLSLMRELLMGSYRVKLANGGER
                     ALKLAALEPQPDLILLDIMMPGMDGYEVCQRLKTNPQTRHIPVVFLTAKAEAEDEKKG
                     LELGAVDYITKPISPAIVLARVKIHLALKAQADFLRDKNDFLEAEVSKRTREVIAIQD
                     VTILVMASLAETRDSDTGNHIRRTQHYVKSLARKIRTHPRFSALLTDSYIHMLFKSAP
                     LHDIGKVGIPDRILLKPGRFEPDEFELMKTHAMLGRDAIEHAERQLGIKVEFLTMAKE
                     IALSHQEKWDGSGYPQGLSGDAIPLSARLMAVADVYDALISRRVYKQGMAHEKAVTII
                     QEGRGTHFDPDLVDAFVVLQQEFQDIAARFADSEPGKGHEMIQFDGMASTAKWIQDLE
                     QKLKMFSSPENP"
     gene            8337..9194
                     /locus_tag="PLB25_00785"
     CDS             8337..9194
                     /locus_tag="PLB25_00785"
                     /inference="COORDINATES: protein motif:HMM:NF024865.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE40163.1"
                     /translation="MQSTRHHLIRSLFDAYIEMYASRDDLLTTRFSENFCGYTGGGDF
                     LVDDRDAWVKITRQDFAQVPGRIEIDTLKLLLQDLSNDIVAATALFNIRLPMPEKDMT
                     RESVRLTLIFRLENESWNIVYSGISIPDQLVRGGEVYPIKGLLEHNLALQRSLEERTR
                     ELAMVNQKLTALTPLKDQVQQRLETRLNQSSDMATIAQALNLSTRTLIRRLQAEGTTF
                     LQIKDQLRRDISLRQLRESQLPVEDIAAQVGFESLTAFHRAFKIWTGSTPLAFRRRGE
                     RRTGDRRQS"
     gene            9503..11560
                     /locus_tag="PLB25_00790"
     CDS             9503..11560
                     /locus_tag="PLB25_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03768 family metallophosphoesterase"
                     /protein_id="HOE40164.1"
                     /translation="MNDKKTNNAPDFRIRGVIGRREFLRYGLGTSVLISSGTSMFGCG
                     GSDSVAAEVVQPYLIDSTVTTTTERMISFPYDLQGLSANEIDKVAKYDSFGYGAWTFG
                     TGLPVVQRVDLMPVGYHNPNPKRNKKFANFFTFSDIHITDKEAPNQLMLLQEFERYAI
                     NNTSIYSPVMMYTTHVLDAAMQTMNVLHKKNPFDFALSLGDTCNSTSYNELRWYMDVI
                     DGKVITPSSGAHLGADTIDYQKPYQAVGLDKSIPWYQTMGNHDHFMIGSFPVDADPSL
                     GFRASYVADTVWAVADTLTPNLSTFPVLFNMQDFKSTPQYYMGLIDGSTPFGNIIHTG
                     LSTSPVFAKGAPKVAADPDRRSLLRTEWVQEFFKTSSGPVGHGFNLVDKNSPPGFTCY
                     SFLPRADVPLKVIVLDDTQSENDGSKDIHGHGYLDAARWTWLKAELAAGQAANQLMII
                     AAHVPIGVATVGTEMEWWLNDVQMATATAAQPQWWLNEPTVAPAQRNATTLTELVTTL
                     QATPNLIMWMAGHRHFNTVKAFKSTNAALPEQGFWHVETSSLRDFPQQLRTFEIYLNS
                     DYTISVVTINVDPAVAAGTPAATSRKYAVAVQQMVKNNLLQSSPNAATMTTNGVTLPM
                     PTIDPTLPQAGDAGTSVGLVNDPSIQFTSLKDVPYNASYNAELFKQLSPAMVAALKAR
                     FPA"
     gene            11572..11760
                     /locus_tag="PLB25_00795"
     CDS             11572..11760
                     /locus_tag="PLB25_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40165.1"
                     /translation="MLTALQATRAGACDRCLRLVSRCKGRVSLYQVRQAGFTRCHGRA
                     GFARISPGDEVHFREHLM"
     gene            complement(11889..12425)
                     /locus_tag="PLB25_00800"
     CDS             complement(11889..12425)
                     /locus_tag="PLB25_00800"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOE40166.1"
                     /translation="MKIDPAVVKLSAENVRAEVLPRPFASRIDPAMIQKIRQRVPIFS
                     GMSPDCLMRTLAIAEHASVKMGDNVFNEGDSGDAFFVLISGEVRVEKSSHGRAVELAK
                     LGAGHCFGEMALVGKNLRSATVRALQDCVTMRFTRQQIDANPESAHIIYRNIASILAS
                     RLESSSVLLADLTGQAKV"
     gene            12669..13862
                     /locus_tag="PLB25_00805"
     CDS             12669..13862
                     /locus_tag="PLB25_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HOE40167.1"
                     /translation="MKLIDSIVSQAANFAALRRDIHAHPELCFEELRTADLVAEKLTE
                     WGIAVHRGLGGTGVVGIIKSGGSSRAVGLRADMDALPMQEANTFAHASSQPGKMHACG
                     HDGHTAMLLAAAQYLSASRDFDGTVYLIFQPAEEGGGGAREMIQDGLFERFPMDAVFG
                     LHNWPGFAVGQFAASAGPVMASSNEFHITIRGKGGHAAMPHLALDPVPVACQLVQAFQ
                     TIISRNLKPIDAGVISVTMINAGEATNVIPNHCELQGTVRTFSVAVLDMIEARMQTMA
                     EHTCAAHGMAVDFVFSRNYPPTINSAPEAEFARRVMADIVGVDQLLVQEPTMGAEDFS
                     YMLLAKPGCYAFIGNGEGQHRDIGHGAGPCMLHNPSYDFNDALLPLGATYWVRLAEAF
                     LLSDQ"
     gene            complement(13901..14665)
                     /locus_tag="PLB25_00810"
     CDS             complement(13901..14665)
                     /locus_tag="PLB25_00810"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HOE40168.1"
                     /translation="MNFLAIDVGNTRLKWALHSAPRRDAPVLAQGAEFLENIDKLAEG
                     DWAQLPAPNRMLGCVVAGDAIKRRVEDQMELWDVSANWVVASAHEAGLVNGYDHPTRL
                     GADRWVAMIGAWHRGLAQGPARPLVVVMVGTAVTVDAIDPTGRFLGGLILPGHGIMLR
                     AMESGTAGLRVPTGNVSEFPTNTSDALTSGGTYAIAGAVERMVQHVRKHCRMEPRCMM
                     TGGAGWKMAPNMSRPFELVDNLVFDGLLEMAARRFV"
     gene            complement(14675..15112)
                     /locus_tag="PLB25_00815"
     CDS             complement(14675..15112)
                     /locus_tag="PLB25_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOE40169.1"
                     /translation="MRHDAPTPHALIQRKADGYAGDISVTLAAQWWQAGEAVLVDVRT
                     DAERTWVGFVPDAVAISWKQWPGMAANADFDANLLAAVPKDKKVLMLCRSGVRSIAAA
                     RRATELGLQAYNILEGFEGDPNELAQRGQRGGWRFNGLPWRQS"
     gene            complement(15569..15787)
                     /locus_tag="PLB25_00820"
                     /pseudo
     CDS             complement(15569..15787)
                     /locus_tag="PLB25_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            15963..>18715
                     /locus_tag="PLB25_00825"
     CDS             15963..>18715
                     /locus_tag="PLB25_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOE40170.1"
                     /translation="MTDLSADFKALALYRPTHKVRFVTAASLFDGHDAAINIMRRILQ
                     SMGTEVIHLGHNRSVEEVVTAALQEDAQGIAISSYQGGHVEYFKYMIDLLKARGGAHI
                     QVFGGGGGVIVHSEIAELQAYGVARIYSPEDGQRMGLAGMIGEMVMRCDKDITGYAPT
                     AIEAIQGHTELSWRALAQLITALENESASGQAKGELSESFKALALAIQAQAATKKIPV
                     LGITGTGGAGKSSLTDELIRRLRLDQGDRLHVAVISIDPSRRKSGGALLGDRIRMNAI
                     NPWSQGLRVFMRSLATRDFGSEISAALPEVIAACKVAGFDLIIVETSGIGQGDAAIVP
                     HVDVPMYVMTPEFGAASQLEKIDMLDFAEFVAINKFDRKGAIDALRDVSKQVQRNKEA
                     WSTPADQMPVFGTMAARFNDDGVTALYQALKVRLAELGLPVTEGSLPRVEVRHSTNQT
                     PVVPAARTRYLAEISDTVRSYKKKTIAQARLAREIQQLNATAQMLQQDDASHGDAKNA
                     VLRLAAERSETQDPAAAKLLAQWPTMQAAYAGEEYVVKMRDKEIRTALTSKSLSGTSI
                     PKVCLPKYEDHGEILKWLMLDNVPGSYPYTAGTFAFKRENEDPTRMFAGEGDPFRTNR
                     RFKLLSSGMDAKRLSTAFDSVTLYGNDPDPRPDIYGKVGNSGVSIATIDDLKVLYGGF
                     DLCSPTTSVSMTINGPAPSILAMFMNAAIDQNLGKFQADNGREPTETETEKIREWVLS
                     NVRGTVQADILKEDQGQNTCIFSTEFSLKVMGDIASYFVHHNVRNFYSVSISGYHIAE
                     AGANPISQLAFTLSNGFTYVEAYLARGMHIDDFAPNLSFFFSNGMDPEYTVMGRVARR
                     IWAVAMKEKYGANERSQKLKYHIQTSGRSLHAQEIQFNDIRTTLQALIAIYDNCNSLH
                     TNA"
BASE COUNT         4032 a         5695 c         5485 g         3503 t
ORIGIN      
        1 gcggctgacc gactgcggct gaccgactgc ggcataattt attgacccgc aaacgcgccg
       61 ccacgtcttg acaaggcggc caagttggtc aaccccggca gcatcacagc acagatcagc
      121 ttctttatgg atgaacaccc ccgaccatga ccacttcgca agccaccgtg cacacctctt
      181 cgctgacttc gctgacgctg ctggcgcggg gcaaggtgcg cgacaactac gcggtgggtg
      241 acgaccgcat cttgatggtc gcgagcgacc gcatcagtgc ctttgacgtg atcatgggcc
      301 aaccgattcc gggcaagggt gtgctgctga cacagatggc gctgttctgg tttgacaagc
      361 tcggccccaa aggcttgaac ctgtgcccga tccacctgac cggtgaggcg cccgagagtg
      421 tggtgacccc cgccgaagtg ccacaggtgg cgggccgctc gatgctggtc aagcgcctgc
      481 ggccgatacc agttgaagcc gtggtgcgcg gctacctggc gggcagcggc tggaaagaat
      541 accagcaagg gcgcagcgtg tgcggtgtgc cactgcccga aggcctgcaa aacgccagca
      601 aactgcccgc gccgatttac accccggcgg ccaaggcagc gatgggcgag cacgatgaaa
      661 acatcagctt tgagcgtacc gtggagatga tcggtgccga cctggccggg cgcatccgca
      721 gcatcagcat cgccatttac caggccgcca gccagatcgc ggcggccaag ggcatcatca
      781 ttgccgacac caagtttgag tttgggctgg accaggacgg cactctggtg ctgatggacg
      841 aggtgctgac acctgactcg tcacgctact ggccagccga gagctatgtt gagggcgaca
      901 acccgccgag ctatgacaag cagtttttgc gcgactggct ggaaaccgcg caggtggcag
      961 gcaaaccctg gagcaaaacg ccaccggcac cgcacttgcc gcgcgaagtg atcgagaaaa
     1021 ccgccgccaa gtaccaggaa gccatggcgc ggctgaccgg ctaaggccgc accagcggcc
     1081 gattgccaag ccggtgcggg tcgccgtgcc gaccggactg acaccctcgc tcgccggtgc
     1141 tgacaagccg cagtaaccag catgccgggc atctggacga accgacggga cacccggatg
     1201 caattatatt gatagcattc agcgcttatt tatcaagcgc tacaggccga tttgctcaaa
     1261 tgtttggtaa tttttcgccc aaccatttca ggtagctggg cgcaacacat gctcaccgca
     1321 tggcagtcaa tgagcccatt gtccgatcag cctgatcagg atgtgaccat gacagcagca
     1381 agcctgatgg cttcagccgg atgcatcgct ttcgtcggct cttgcctggc acctggggca
     1441 accgtcaccg ccgatacacc gtgccaagcc agacaaattg cccagggtaa atttgttctt
     1501 atggcatctt cgtcattccc gtctgcgcgg gaatgacggt aaatcaacga cttagcgaaa
     1561 gaacaaattt gcccagcaaa ttgccgcagc agcattgcca aaacgtgcgc cgatggttga
     1621 caatatttca acctgagccg gacgtaccgg gatcgatgag ccttcgtcgc gagggcgcga
     1681 ttcctgcaca aacaggcggt gcctacttga atttgtagga gtcgcgccct cgcggcgaat
     1741 gacattgaat gtttgaattc ttcgcatgat atgcaaagac ttgacttgtt agagaccaga
     1801 ttccttcgtt ggacgggttc gcagcgctgt cacccagtgg gtgagcccga ttaaagccaa
     1861 agcagcaaat gctcataaag acttacagat gaaaacaagc ggtcaaccga ggaatctggg
     1921 tgcaatgttt ctcgccaact tcaccgatgt cgataaggcc cgcagcatcg ccaataggcg
     1981 ctggctgcgt ccagcgccag ttgtcaaggc gaatggtcgg tcggcattgg cccttgcgcc
     2041 caaactgacg gacctgacgg gcatttttca ctggcccggc aggctggttg cactgcaacc
     2101 aaaggggctt ccaggtggtg ctggaagcgt ggtcaatggt ctatctgcgg cctcgggcgc
     2161 ttggctgaaa tgagtcgcct gcgcatcaag agcttgcgct ttctgctggg cttccatctt
     2221 cccgtggtag ccattgcact gttgaccggg ctgctgagcc tgaattcgtt tgccgatttg
     2281 ccagaaggcc accttcgcgc cagcccgcag ccggaacatg acacccagcg tatcgccgcc
     2341 atagcgggaa tcaaccagca aatgggccgg agtcaaattg aagtgggcag catgctgcaa
     2401 aaagccgcca gcgggcaggc aaacgaggcg cagatgtacc gctttcacgc cgcagtgctg
     2461 cgccgcctgt cggtgttgca acaagcgatg gctggcattg cagatgaagt cgcctacaac
     2521 ctgccgctga tgcaggccgg tcggaatttc gggaatgata aaagtaacgt catcatggcc
     2581 accagccttg cggcagttga cccggacagc gccatgcgcc acgcgcttgc ggccatgcaa
     2641 gggctggtga tgatggcaga acagtctccc tcaatcgcca acaccatcgc tgtcgagatg
     2701 gccgagcgca gcaaggccca tgcccagtcc tttgagcaac actcggtgca aacgggtttg
     2761 accggcatcg ttgtgatggc gttgctgctg ctatggctgt atgtctcgca gcgggtgacg
     2821 cggcggctgt cgcggctggt accggcgttg cgtgggtttg cgctggacga tattgagccc
     2881 cgattgctgg cgcaggtgtg ccagttgagc ggccaacaga acaggtgctg tgtaacctgg
     2941 cgctgtcgct gctggctttt cgggaaagcg tcattgcaag aaagacggcg caatgtgatt
     3001 tgggggaacg tatgaacgtg ctgtcctgtc tctacgatgt catgcacctc accgaagacc
     3061 cgggtcgtga ccaggacgag atgcttgagg cggttttgca gcgcctgccg ggcgccatgc
     3121 gttacccgga catcgcggct ggctggatcg acttcagcgg tcgccgctac ggcagcagcg
     3181 cttcgggcga acagttgagc gtgtgctttg gcggcacgcc caagcagccc gacctgcttg
     3241 gcgtgactta catcgcgccc ctgccgaccg atgcccgcga gccgtttctg gcacaggagc
     3301 ggtctctttt tgaggccctg ggcaagcgcc tgaccaacgt catgcaacgc aggcgcactg
     3361 agcgctcgct gcagcaggct gaccgagcat tgcgcacagc caggcaatgc ggccagcttt
     3421 tgaaccgcgc tgagcacgaa gatcaattga tgcaggacac ctgtcggctc gcggtcgagg
     3481 ttggcggcta ccggatggcc tgggtcggtt tggccgagaa cgatgaagcc cgcagcgtcc
     3541 gaccggtggc ccgcgcaggc ttcgatgaca actatcttga tgccgcacag atttcctggg
     3601 cagacggcgc acgcggacgc gaaacaaccg ggacggccat ccgcgagcgc cgcaccgtgg
     3661 tgacgcaaga catcctaacc aacccggacc tggccccctg gcgcgaggcg gcaatgcgct
     3721 gcggttatgg cgcggccatt gcaatgccct tgctggccga ggcagatcaa tgcatcggtg
     3781 ccctgtgcct gcatgccggt gcgagcgacg ccttcagtga cgcagaagtc caacacctcg
     3841 ttgaaatggc cagtgacctg tcctatggca ttcggaccct gcgcacgcgc agcgcattga
     3901 acagcagcct tgttgaactg cgcaaactgt cgctggcggt cgcgcaaagc ccgaacgcca
     3961 tcatggtgac cagcatggag cctcgcatcg agtacgtcaa cgaggccttt acccgcatta
     4021 ccggcttcac ccgcgaagag gtgctgggcc agaacccccg gctgctccag tccggaaaaa
     4081 cacccgctac gacctaccag gacatgtggc agacgctgct cggcgcaaaa atctggaccg
     4141 gcgagttcat caaccacacc cgccagaaag aggaactgat cgaggcggcg atcatcgtcc
     4201 cgctggtcga aagcgatggg caggtttcgc actacgtggc gatcaaggaa gacatcaccg
     4261 acaagaagcg catgcgcgat gagttggacc gccatcgcct gcatctggaa gaactggtcg
     4321 cccttcgcac cgggcagctt gaagccgcac tgcagcaaca agtcgcgctg tttgaagcgg
     4381 ccagcgttgg cattgctgtg ctgcgagagc ggatcatcgt gcgctgcaac cggaccctcg
     4441 acgagatgat ggactatgtc ccaggcgagc agattggcca gagcgtgcgc atctggtacc
     4501 cggaccagtc tgcttacgcc aaggccagcc aagaactgta cgaccggccc aactgcggtg
     4561 aagttcgcac ggtcgagcgt gacctggtgc gcaaggacgg cagccacttt tgggcacgcc
     4621 tgtcagtgcg cagcattgac cccaccggcc tgtccaaaga catggtctgc attgtggaag
     4681 acctgacgca agagcgtgcg gtgattgctg aaatcagcca ggcgcgcctc caggccgagt
     4741 ccgccaaccg cagcaagtac gactttctgg ccaatatgag ccacgaaatc cgcacgccca
     4801 tgaatgccat cattggcatg agccacctgg cgctgcaaac cgatctcgac aagaagcagc
     4861 gcaattacat tgaaaaagtg catcgctcgg gccaaagcct gctcggcatc atcaacgaca
     4921 tcctggactt ctccaggctt gagaccggca aaatgagcat ggagaccgcc gccttcaatc
     4981 tggaagatgt gatggacaac ctggccaatc tggtcggttt gaagaccgag gagaaggggc
     5041 tggagctgct ttttcacacc gcaccggacg taccgacagc cctcattggc gactcattgc
     5101 gacttggcca ggtcctcatc aacttgagca acaacgcggt caaattcacc gaaaagggtg
     5161 aaatcgtcat cggcatcgac aaggttgccg accatgagga cggcgttgag ctgcatttcc
     5221 gggtacgcga caccggcatc ggcatgaccc ctgagcaatg cggcaagatg ttccacggct
     5281 ttggccaggc cgacacctcc accacccgca aatacggcgg caccggcctg ggcctggcca
     5341 tctgcaagaa tttggttgag caaatgcacg gcaagatctg ggtcgagagc gtggccgggg
     5401 ttggctccac ccttcacttt catgtccggt tgggcgttca ggcccaagcg caagtctggc
     5461 ggatgttcaa ggccgagcaa ttgcagggcg tgcgtgcgct ggtggtcgat gacaacgcag
     5521 cggcccgtga aatcctgtcc gccatggtca ccacctttgg cctgcaagtc gatgttgccc
     5581 aagacggcgc acaggccctg cacctgatcg ccgcggctga caaaaaggct cagccctacg
     5641 aactggtgtt gatggactgg aaaatgccgg tgatggacgg gcttgctgcg gcacaccatt
     5701 tgcacagcga acagttgagc cgcgtcccca cggtcatcat ggtcaccgcc catggccgcg
     5761 aagatgtcat ggcaagcgcc cgcgaacacg gcgtagcggt gcaaagcgtg ctgaccaagc
     5821 cggtgacacc ttccgttctg ctcaaagcca ttggtgaagc gctgggcaaa gacaccaacg
     5881 tcacgacccg agaagaagcc cgcgcagacg acaatgccag ggcaatcgaa aagctcaagg
     5941 gtgccagggt gctgctggtt gaagacaacg aaatcaacca ggagctggcg atggaactcc
     6001 tgaccagctc tggcatgacg gtggtgctgg ccaaccacgg tcaggaggcc ctggacattc
     6061 tgcgtcagga ccggcatttt gacgctgtct tgatggactg ccagatgccg gtgatggacg
     6121 gctacaccgc cacccgcgag atccgccgaa acccggtttt caaagacctg ccgatcattg
     6181 ccatgaccgc caacgccatg gcgggcgaca aagaaaaggt gctgcaagtc ggcatgtgcg
     6241 accacctggc caaaccgatc gatgtggcgg ccatgtttgc gacgctggcc aaatggattg
     6301 acccgtctgc aaccgttgca gacgcctctt tagctataaa aaaagaagct gtcagcgcaa
     6361 ttgagacggg cgctacaggc atttttgatc aattgtcccg ggcgggcatc gatacccggt
     6421 ctggactggc agcggccatc aacaaggagc cgctgtaccg gcgcctgctg ctcaagttcc
     6481 gcgatggcca gggcgcgttt gccgatctgt ttgcagccgc ccgcagcggg tccgacagca
     6541 ctgcggcgca gcgctgcgcc catacgttac gcggcacggc cgccaccata ggtgccaggg
     6601 gcgtgcagga agctgcggca aagctggagc aggcctgcca gcagcaggcc gccgatgcgc
     6661 agattgacga cctgctgcgc ttggtgctgg aggaacttca gcccgtgctt gctgcgctgc
     6721 aaacgttcgg cagcgacgta gcaggcgtgc cggtgcactt gccagtcgca gttgatgctg
     6781 tcaagctgtc tggcgtgcgc gccagcctga tggaactgct ggacctgggt gacgcgcgtg
     6841 ccatcgacct atttgacgcg catgaagccc tgttccgtgt cgcttacccc gcgcaatgga
     6901 agacaatcgc cagcagcgta catgactttg actttgaatc ggcactggcg ctgatccagc
     6961 aatcctcttg agaccaccca ccaaggagcg acagtcgtat ggatcatgca gaagaaggga
     7021 ttgtgaaagc aaccattctg gttgtcgatg acacgcccga caacctgtcg ttgatgagag
     7081 agctgctcat gggcagctac cgggtcaagc tggccaacgg tggcgagcgc gccctgaaac
     7141 tcgccgcgct ggagccacag cccgacctga ttctgctcga catcatgatg cccggcatgg
     7201 acggctacga ggtctgccaa cggctcaaga ccaacccgca aacccggcac attccggttg
     7261 tttttctcac cgcaaaggcc gaagcggaag acgaaaaaaa ggggctggag ttgggcgcgg
     7321 tggactacat caccaaaccc atcagcccgg ccatcgtact ggcgcgtgtg aagattcacc
     7381 tggccctgaa agcgcaggcc gatttcctgc gcgacaaaaa cgactttctg gaagccgagg
     7441 tcagcaaacg cacacgtgag gtcattgcga ttcaggacgt gaccattctg gtgatggcat
     7501 cactggcgga aacgcgcgac tccgacaccg gcaaccacat ccgccgaacc cagcattacg
     7561 tcaagtcact ggccagaaaa atcagaactc acccacgctt ttctgcgctg ctcactgaca
     7621 gctacatcca catgctgttc aagtccgcgc cgttgcatga catcggcaag gtgggcattc
     7681 cggaccggat tttgctcaag cctggccggt ttgaacccga tgagttcgaa ctgatgaaga
     7741 cccacgccat gcttggccgg gacgcgatcg agcacgcaga gcggcagctc ggcatcaaag
     7801 tggagttcct gaccatggca aaggagattg cgctgtcgca ccaggaaaag tgggatggct
     7861 cgggttatcc gcaagggctc tcaggtgatg cgattcctct ttcagcacgg ctgatggccg
     7921 ttgccgacgt gtatgacgcg ctgatcagcc gccgtgtcta caagcagggc atggcccatg
     7981 aaaaagcggt gaccatcatt caggagggcc ggggaactca ttttgatccg gacctggtgg
     8041 acgcgtttgt tgtattgcag caagagtttc aggacatcgc agcaaggttt gcagactcgg
     8101 agccgggtaa agggcacgaa atgatccagt ttgacggcat ggcaagtacc gcaaagtgga
     8161 ttcaggattt ggaacagaaa ctcaagatgt tctcaagtcc ggaaaatcca tgatcgtgcg
     8221 tgaaacgaag aaacgcagat gccacccacc agagtaccaa gccaaggtgg ggttggaggc
     8281 tgtgcccagc ggcaagacca tcaaccagat tggccaggag tttgacgttc gcactcatgc
     8341 aatcgacccg tcaccacctc attcgatcat tgtttgatgc gtacatcgaa atgtacgcat
     8401 cgcgcgacga tttgctcaca acacgattca gcgaaaattt ttgtggctat accggtggcg
     8461 gcgactttct ggttgatgac agggatgcct gggtaaagat cacccggcaa gattttgcgc
     8521 aggtgcccgg tcgtatcgaa attgatacgc tcaagctcct gctgcaggac ttgagcaacg
     8581 atatcgtggc agcgaccgcg ctctttaaca ttcggctgcc catgcccgaa aaggacatga
     8641 ccagggagtc ggtgcgcctg acgctgattt tccggcttga gaacgagagc tggaacattg
     8701 tttacagcgg catctcgatc ccggatcaac tggtgcgagg tggtgaggtc tatccgatca
     8761 agggactgct tgaacacaat ctggcgctgc aacgctcgct ggaagagcgc acccgcgagc
     8821 tggccatggt gaatcagaag ctgacggcgt taacgccatt gaaagatcag gttcaacagc
     8881 gcctggagac gcggctgaac caaagcagcg acatggcgac gattgctcag gcgctgaact
     8941 tgtctacccg cacgctgatc aggcggcttc aggccgaggg cacaaccttc cttcaaatca
     9001 aggaccaact gcgccgggac atctcgcttc gccagctcag ggagagtcaa ctaccggttg
     9061 aagacatcgc ggcacaagtc ggctttgaaa gcctgacagc gttccaccgc gccttcaaga
     9121 tctggaccgg cagcacgcca ctggccttca gacgtcgggg tgagcgacgc acgggtgacc
     9181 ggcggcagtc ttgacgcaga cagtttgcgt tagatcaatg tctgccaagt gtggcgtaaa
     9241 cggcgaattt tttggcctga acaggcaaca aatcttccac acctctgcct agacttgctg
     9301 cacagcgcaa aaggcttttt ggcaaatcca gggcaatgca ttcattgcat gactggcgtg
     9361 ttaacgcaaa aaaatgagcg actctcaatg aaaaccagcc ccaggaacac gacggtagct
     9421 tggccaaagc ggttgatgcc ggggcttacc gcaacacaag cttgcccgac aaatcagttt
     9481 ttaagtcaac gaaaggtgcc ccatgaacga taagaaaaca aacaatgccc ccgatttcag
     9541 gatccgtggc gttatcggcc gtcgtgagtt tttgcgctat ggcctgggca cttcggtgtt
     9601 gatctcttct ggcaccagca tgttcggttg tggcggtagt gacagtgtcg cggctgaagt
     9661 cgtccagccc tatttgattg acagcactgt gaccaccacc actgaacgca tgatctcgtt
     9721 cccctacgac ttgcaagggc tgtctgccaa tgagatcgac aaggtggcca agtacgattc
     9781 ctttggttat ggtgcctgga cttttggcac cggtttgcca gtcgtgcaac gggttgacct
     9841 gatgccagtt ggctaccaca acccgaaccc gaaacgaaac aagaaatttg ccaatttttt
     9901 cactttctcg gacattcaca tcaccgacaa ggaagcgccc aaccaactca tgctgttgca
     9961 ggagtttgag cgctacgcca tcaacaacac atcaatctat tcgccggtga tgatgtacac
    10021 cacgcatgtg ctcgatgctg ccatgcagac catgaacgtc ctgcacaaga aaaatccgtt
    10081 tgactttgcg ctctcgctgg gcgacacctg caacagcacc tcttacaacg agcttcgctg
    10141 gtacatggac gtgattgacg gcaaggtcat cacaccgagc tcgggcgcgc atctgggtgc
    10201 cgacaccatc gactaccaaa aaccctatca ggcggtgggg ctggacaaat ccataccctg
    10261 gtaccagacc atgggcaacc atgatcattt catgattggc tcattcccgg tggatgccga
    10321 ccccagtttg gggtttcgtg cgtcctacgt tgctgacacc gtgtgggcag tggctgacac
    10381 actgacaccc aacctgagca catttccagt cctgttcaac atgcaggact tcaaatcgac
    10441 accccagtac tacatgggtc tgatcgatgg cagcacgcct tttggcaaca tcattcacac
    10501 cggcttgagt accagcccgg tatttgccaa aggtgcgccc aaggtggcgg ctgacccgga
    10561 ccggcgatca ttgctgcgca ctgaatgggt gcaggagttc ttcaagacca gcagcgggcc
    10621 ggtcgggcac ggcttcaacc tggttgacaa aaacagtccg ccgggattta cctgctacag
    10681 ctttttaccc agagccgatg tgccgttgaa ggttatcgtg ctcgatgaca cgcagtcaga
    10741 gaacgacggc tcgaaggaca tccacggtca tggctacctt gacgcggcac gctggacctg
    10801 gctgaaggcg gaactggccg ccggtcaggc tgccaatcag ctcatgatca ttgccgctca
    10861 tgtgccgatc ggtgtggcca ccgttggaac cgagatggaa tggtggctca acgatgttca
    10921 gatggcgacg gccaccgcag cacagccaca atggtggctc aatgagccca cggtggcacc
    10981 agcgcaaaga aacgcgacga ccctgacaga actggtcaca acgctgcaag ccacccccaa
    11041 cctgatcatg tggatggcag ggcaccgcca cttcaatacc gtcaaggcct tcaagtcgac
    11101 caatgcagcc ttgcctgagc agggtttctg gcatgtggag acttcatcgc tgcgcgattt
    11161 tccccaacaa ctgcgcacct tcgagattta cctcaacagt gactacacca tctccgtcgt
    11221 caccatcaac gttgacccgg ctgtcgccgc tggcacaccc gcagccacct cacgcaagta
    11281 cgccgttgca gtgcaacaga tggtcaaaaa caacctgttg cagagttcgc ccaatgcggc
    11341 gacgatgacg acaaacggcg tgacactgcc gatgcccacc attgatccaa ctttgccgca
    11401 agctggtgac gcgggcacca gcgtcgggct ggtcaatgac ccgtcgattc aattcaccag
    11461 tctgaaggac gtgccctaca acgcctccta caacgccgaa ctgttcaagc aattgagccc
    11521 ggcaatggtt gccgccttga aggccagatt tccagcctga aaccgagccc gttgctgacc
    11581 gccctgcagg caacgcgggc cggtgcgtgc gaccgctgcc tgcggctggt gtcgcgctgc
    11641 aaaggccggg tttcgctgta tcaggttcgc caggctggtt tcactcgctg ccacggccgc
    11701 gcaggctttg cccgcatcag ccccggtgac gaggttcact ttcgtgagca tctgatgtga
    11761 tctggagcaa tcgtgtccgt gagcttgcct gactgatggc gcatcggttg cgaaccgact
    11821 gaatcggcgt tgtggcagtt gattttggcg gcgaccttgg cgtgaaagcg ccggcgctca
    11881 gggcctgatc aaacttttgc ctggcccgtc aggtcggcca gcaagacgct tgagctttcc
    11941 agtcggctgg ccaggatgct ggcgatgttg cggtaaatga tgtgggcgct ttcggggttg
    12001 gcatcaatct gctggcgtgt aaaccgcatc gtcacgcagt cttgcagggc tcgcacggtt
    12061 gctgagcgca aattcttgcc aaccagcgcc atttcaccaa aacaatgacc agccccgagc
    12121 ttggccaact caacggcacg cccatgactg ctcttttcca cgcgaacctc gccactgatc
    12181 aagacaaaaa atgcatcgcc agagtcacct tcgttgaaca cgttgtcgcc catcttgacc
    12241 gacgcgtgct cggcaatggc cagcgtgcgc atcaggcaat cggggctcat gccgctgaaa
    12301 atcggcaccc gttgcctgat cttttgaatc atggcggggt caatcctgga cgcaaatgga
    12361 cgtggcaaga cctcggctcg cacgttttcg gcagacagct tgacgacggc tggatcaatc
    12421 ttcatggctg ccaatagtac acggctgcgc cccgggcacc agtcgccccg gcggccgggc
    12481 ctgatgggga atttgcccca acccacccgg cttgaggata tgccgacatc ttgcacagtg
    12541 ctcccggcaa gtcctgatgc tgcagcacgc tggtgagagc tgacacgcca actcgggccg
    12601 gtgatggatg acccggcctg acgcggcggg ttggcgaaac ctttagactc ggtctggagg
    12661 agccaagcat gaaactgatc gattccatcg tctctcaggc ggccaatttt gctgccttgc
    12721 ggcgtgacat tcacgcccac cccgagttgt gctttgaaga gttgcgcacc gccgacctgg
    12781 tggccgaaaa gctcaccgag tggggcattg cggtgcaccg tggcctgggc ggcaccgggg
    12841 tcgtcggcat catcaagagc gggggttcca gccgggccgt cggtttgcgt gccgacatgg
    12901 acgcactgcc gatgcaagag gccaacacct ttgcccacgc cagcagccag cctggcaaga
    12961 tgcacgcctg cggtcacgac gggcacaccg ccatgctgct ggcagcggcc cagtacctca
    13021 gcgccagccg cgattttgat ggcaccgtct atttgatttt ccagccagcc gaagagggcg
    13081 gcggcggtgc ccgcgagatg atccaagacg gtttgtttga gcgttttcca atggacgcgg
    13141 tgtttggcct gcacaactgg cctggctttg cggtcggcca gtttgccgcc agcgccgggc
    13201 ctgtgatggc ttcaagcaac gaatttcaca tcaccattcg tggcaaaggt ggccacgccg
    13261 ccatgccgca cctcgcgctt gatccggtgc cggtggcctg ccaattggtg caggcttttc
    13321 aaaccatcat cagccgcaac ctcaaaccaa ttgacgcagg cgtgatttcg gtcaccatga
    13381 tcaacgctgg cgaggccacc aacgtgattc ccaaccattg tgagctgcag ggcacggtgc
    13441 gcaccttctc ggttgccgtg ctcgacatga ttgaggcgcg catgcaaacc atggccgagc
    13501 acacctgtgc cgcccacggc atggcggttg actttgtttt ttcgcgcaat tacccgccca
    13561 ccatcaacag cgcgcccgaa gccgaatttg cccgccgggt gatggccgac atcgtcggcg
    13621 tggaccagct tctggtgcag gaaccgacca tgggggccga agacttttcc tacatgttgc
    13681 tggccaagcc gggttgttat gcctttattg gcaatggtga aggccagcac cgcgacatcg
    13741 gccacggcgc tggcccgtgc atgctgcaca acccgagcta tgacttcaac gatgcgctgc
    13801 tgccgctggg ggccacttac tgggtgcgtc tggcagaggc ttttttgcta tctgatcaat
    13861 agctgctagc gcaagcagca caagggctac aggccttttt ttatacaaat cggcgcgccg
    13921 ccatttccag cagcccgtca aacaccaggt tgtccaccaa ctcgaacggg cgcgacatat
    13981 tcggtgccat cttccagcca gcaccgcccg tcatcatgca gcgcggctcc atccggcagt
    14041 gcttgcgcac atgctgcacc atgcgctcga ccgcaccggc aatggcgtag gtgccaccgc
    14101 tggtcagcgc atcgctggtg ttggttggaa actcgctcac attgccggtg ggcacccgca
    14161 gaccggcggt gccagactcc atggcccgca acatgatgcc gtgccccggc aaaatcagcc
    14221 cgcccaaaaa gcggccggtc gggtcaattg catcgaccgt gacggcggtg ccgaccatca
    14281 ccaccaccaa cgggcgcgcc gggccttgcg ccagcccccg gtgccaggca ccgatcatcg
    14341 ccacccagcg gtcggcacca agccgggtcg ggtggtcgta gccgttgacc aggcccgcct
    14401 cgtgggcact ggcgaccacc caattggcgg aaacgtccca cagttccatc tggtcttcaa
    14461 cccgccgctt gatcgcatcg cccgccacca cacagcccag catccggttt ggggctggca
    14521 actgggccca gtcgccctct gccagtttgt caatgttttc cagaaactcg gcaccctgcg
    14581 ccagcaccgg ggcatcgcgc cggggggcgc tgtgcaaagc ccatttcaga cgtgtattgc
    14641 caacatcaat cgccaaaaaa ttcatgatga cgcttcagct ttggcgccag ggcagaccgt
    14701 tgaagcgcca gccaccgcgt tgaccgcgct gggccagttc attcgggtcg ccttcgaaac
    14761 cctccagaat gttgtaggcc tgcaagccga gttcggtggc ccgtctggct gccgcaatcg
    14821 agcgcacgcc gctgcggcat agcatcagca ctttcttgtc cttgggtacg gcagccagca
    14881 agttcgcatc gaagtctgca ttggcggcca tgccaggcca ttgcttccag gagatggcca
    14941 cagcatcggg cacaaagccg acccaggtgc gctcggcatc ggtgcgcaca tcaaccagta
    15001 ccgcctctcc ggcttgccac cactgggctg ccagcgtgac ggaaatgtca ccggcgtagc
    15061 catctgcctt gcgctgaatc agcgcatgcg gtgtcggcgc gtcgtggcgc aaacccaggt
    15121 gcaggttggc cggcaccgcc tcagccatgc gtttcggggg tggcagatgc aaggcttcca
    15181 tgatggcaac gaactcaggc agcgtcttgc ccgccaccct ggcattgctg gccttttcgt
    15241 gaccaatgct cgaatggctg cgcccctggt agtcatggcc gggccagacg gtgctggcat
    15301 cgggcagctt gaacagcact tcggtcaggc tgtggtacat ctgggcggcg ctgccggact
    15361 gaaaatcggt gcggccgcaa ccgtcgatca gcaaggtgtc gccagtgaaa acatggttgc
    15421 gccagacaaa gcacatgctg ccagcggtgt ggccgggggt cagcagggcg cgcacctgct
    15481 ccgagccaaa gttcagcgtg gcgccgtcct tgagcaatac cgacgcgctg ctgatgccgc
    15541 agccctcggg ggcggtggtt ctggcgccgg ccagctcggc gagttgcccg gcactggtga
    15601 tgtggtcagc gtgggcgtgg gtttccagcg accagatcag ccgcaaaccc tgttcgcgca
    15661 ataccgtcag gtcccgggtg atctgggtat cgaccgggtc gatgatcacc gcatcccggc
    15721 tgaccgggtc aaacaggacg taggtgaagg tggaagttgc ggtgtcaaaa agctggatgg
    15781 gtttcatggc tacaaagggc ttatgcatca gaagttgaaa tgtaccgtga aagccgggtg
    15841 accgggaaaa cccgctaaca tcgaggtccg cattgacgtt tacgtcaacg tcaagtcagc
    15901 gatacctgct ttgatcgccg acttcgccaa aacagcaatt ccaccctacc gagaccccca
    15961 tcatgaccga cctgtccgcc gacttcaagg ctttggccct ttaccgccca acccacaagg
    16021 tgcgttttgt taccgctgcc agcctgtttg acgggcacga tgcagccatc aacatcatgc
    16081 gccgcatttt gcaaagcatg ggcaccgagg tgatccacct cgggcacaac cgcagtgttg
    16141 aagaagtggt cactgccgcc ctgcaggaag acgcgcaggg catcgccatc agctcttacc
    16201 agggcgggca tgtggagtac ttcaagtaca tgattgacct gctcaaggcc cgtggtggcg
    16261 cgcacatcca ggtatttggc ggcggcggcg gtgtcatcgt gcacagcgaa attgccgagc
    16321 tgcaagccta tggcgtggcc cgcatctaca gccccgaaga cggccagcgc atgggcctgg
    16381 ctggcatgat tggcgagatg gtgatgcgct gcgacaaaga catcaccggc tacgcgccaa
    16441 cggccatcga ggccattcag ggccataccg agttgagctg gcgcgcgctg gcgcagctca
    16501 tcaccgcgct ggaaaacgaa agcgcctcgg gccaggccaa aggtgaatta tcagagtctt
    16561 ttaaggcgct agcccttgcc atacaagcgc aagcagctac taaaaagata ccagttctgg
    16621 gcattaccgg cactggtggc gcgggcaaat ccagcctcac cgacgagctg attcgccgcc
    16681 tgcgccttga ccagggcgac cgcctgcatg tcgccgtcat cagcatcgac ccgagccgac
    16741 gcaagagtgg cggtgccctg ctgggcgacc gcatccgcat gaacgccatc aacccctgga
    16801 gccagggttt gcgggttttc atgcgttcgc tggccacgcg cgactttggc agcgaaatca
    16861 gcgccgccct gcctgaagtg attgccgcct gcaaggtggc cggttttgac ttgatcattg
    16921 tcgaaaccag cggcattggc cagggcgacg cggccatcgt gccgcatgtc gatgtgccga
    16981 tgtatgtgat gacccccgag tttggcgcgg ccagccagtt ggaaaaaatc gacatgctcg
    17041 actttgccga gtttgttgcc atcaacaagt ttgaccgcaa gggggccatt gacgcgctgc
    17101 gcgatgtgtc aaagcaggtg cagcgcaaca aggaagcctg gtcaaccccg gccgatcaaa
    17161 tgcctgtgtt tggcaccatg gcggcgcgct tcaatgacga cggtgtcacc gcgctgtacc
    17221 aggccctgaa agtgcgcctg gccgagctgg gcttgccggt cactgaaggc agcttgccac
    17281 gggttgaggt gcgccacagc accaaccaga ccccggtggt gccagcagcg cgtacccgct
    17341 atctggcaga aatcagcgac acggtgcgca gctacaagaa aaagaccatc gcccaagccc
    17401 gcctggcgcg tgaaatccag caactcaacg ccaccgcgca aatgttgcag caagatgatg
    17461 ccagccatgg cgatgccaaa aacgccgtgc tgcgcctggc ggcggagcgc agcgaaactc
    17521 aggacccggc agccgccaag ctgctggccc agtggccaac catgcaggcg gcctatgcgg
    17581 gtgaagaata cgtggtgaaa atgcgcgaca aggaaattcg caccgcactc accagcaaga
    17641 gcctgagcgg caccagcatc cccaaagtct gcctgcccaa gtacgaagac catggcgaaa
    17701 tcctcaaatg gctgatgctc gacaacgtgc ccggcagcta cccctacacc gccggcacct
    17761 tcgccttcaa gcgcgaaaac gaagacccaa cccgcatgtt tgccggcgaa ggcgacccgt
    17821 ttcgcaccaa ccgccgcttc aaattgctca gctcgggcat ggatgccaag cgtcttagca
    17881 ccgcctttga ctcggtcacc ctgtacggca acgaccccga cccgcgcccg gacatctacg
    17941 gcaaggtcgg caacagcggc gtgtcaattg ccaccatcga cgacctgaaa gtgctctacg
    18001 gcggcttcga tctgtgcagc ccgaccacca gcgtcagcat gaccatcaac ggcccggcgc
    18061 ccagcattct ggcgatgttc atgaatgccg ccatcgacca gaacctgggc aagttccaag
    18121 ccgacaatgg ccgtgaaccg accgaaaccg agaccgaaaa aatccgcgaa tgggtgctga
    18181 gcaacgtgcg cggcacggtg caggccgaca ttctgaaaga agaccagggg caaaacacct
    18241 gcatcttcag caccgaattc agcctgaaag tgatgggcga catcgccagc tactttgtgc
    18301 accacaacgt gcgcaacttc tacagcgtgt cgatttcagg ctaccacata gccgaagccg
    18361 gtgccaaccc gatcagccaa ctggccttta ccctgagcaa cggctttacc tacgtcgagg
    18421 cttacctggc acgcggcatg cacattgacg actttgcgcc caacctgagc ttcttcttca
    18481 gcaacggcat ggaccccgag tacaccgtca tgggccgcgt ggcgcggcgc atctgggcgg
    18541 tggcgatgaa agagaaatac ggcgccaacg agcgcagcca gaagctcaaa taccacatcc
    18601 agaccagtgg ccgcagcctg cacgcgcagg aaatccagtt caacgacatc cgcaccacct
    18661 tgcaggcgct gatcgccatt tacgacaact gcaacagcct gcacaccaac gcctt
//
LOCUS       DAOSLL010000010        18151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_3311_length_18151_cov_4.847701, whole genome shotgun sequence.
ACCESSION   DAOSLL010000010 DAOSLL010000000
VERSION     DAOSLL010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 18151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18151
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3311_length_18151_cov_4.847701"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /gene="dctP"
                     /locus_tag="PLB25_00830"
     CDS             <1..518
                     /gene="dctP"
                     /locus_tag="PLB25_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOE40171.1"
                     /translation="KIRVVGSPIYSDMFSAMGANPTQMSWADAQPALSSGAVDGQENP
                     LFLFTVLKMQNVGQKFLTAWGYVADPLIFVVNKDIWNSWTPADQALVRQAALDAGKQE
                     IVLARKGLVEADKPLFKDIAAMGVTVTQLSPAEREAFVKVTRPVYEKWKATVGADLVK
                     AAELAIAARKK"
     gene            811..2058
                     /locus_tag="PLB25_00835"
     CDS             811..2058
                     /locus_tag="PLB25_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE40172.1"
                     /translation="MDHMTASRPPKKPRHLQASDLRCVALLATEATQAVTAISEGVHQ
                     SILGTLGAPSGDTRDKTWGLTGLIYQSINGVTQLIGKGVASALTRLEPLLQRLEGEAD
                     ESDERTAVIAALNGVMGDRLQASSNPLATPMTLRHAGAVLDPQQPALASASGKLLLVI
                     HGLCMNDLQWTTRHEEQTVNHADALAKALACTPVYLRYNSGLHVSQNGHALATLLEQL
                     VANWPVPLTEVSVLVHSMGGLITRSALHYATQANLQWPKYLKNIVFLGTPHHGAPLER
                     AGNWVDVVLGSTPYTRPFAKLGQLRSAGVTDLRYGHVLDVDWQGHDRFRRKPDSRQPV
                     PLPQDVACFAVAATTMASRSSLADRLIGDGLVPLHSALGQHDEADRQLVFAPERQLIV
                     YGTNHMQLLGKPEVTRQLLAWLG"
     gene            2153..3049
                     /locus_tag="PLB25_00840"
     CDS             2153..3049
                     /locus_tag="PLB25_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HOE40173.1"
                     /translation="MTTLPALPCYLNGEFTTLPNAKISVMDRGFIFGDGVYEVVPVYQ
                     GHLFCFANHMARLERSLKELRMNNPYSRPQWTQIAMRLIADYAQSTGATGQNIDQLIY
                     IQVTRGVAMRDHVMPTDITPTVFVMTSKMTTPSSEQRRLGVVCVTADDFRWEKAHIKS
                     TSLLGAVFARQISFDQEAVETIMFRDGFLSEAAACNVWVVKDGRLLGTPKDNLVLEGI
                     RYGLIEEICAKQGIAFELRRVTRAEVMAADEILLSSATKEVLPVTLLDGQPVGNGAPG
                     PVYAQLYAGYQEAKAELLQTPA"
     gene            3124..3366
                     /locus_tag="PLB25_00845"
     CDS             3124..3366
                     /locus_tag="PLB25_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HOE40174.1"
                     /translation="MAVLTIRNVDDTIKSALRIQAAQRGVSMEEEARRILREALTRAS
                     PSTPMGQRLLRRFADVASDEFALPQRQPPRTQAFED"
     gene            3373..3801
                     /locus_tag="PLB25_00850"
     CDS             3373..3801
                     /locus_tag="PLB25_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018004414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOE40175.1"
                     /translation="MRLLDTNVLSEFMRQLPAPQVVAWLDAQPTSAMFTSAISRAEIE
                     LGLALMPAGKRQLALAQSARAMFDTEFADMCLPFDETAAVRYAQVVTVRSRAGHPISV
                     EDAQIAAIALVHKLELVTRNVADFTGIEGLTVVDPWHLDP"
     gene            3944..5353
                     /locus_tag="PLB25_00855"
     CDS             3944..5353
                     /locus_tag="PLB25_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40176.1"
                     /translation="MASPTSVATFELAVKKHGGGQGFVDLFWPGMLLVEHKSRGRDLN
                     QAFDQALGYFPGLPERDLPQLIVVCDFARFRVHKLPSGEALEFPLAELHQNVGLFGLL
                     ASYKQQVIRPQDPVNFLAAEKMGRLHDALKASGYGQEAAPGVAAGHALEVLLVRLLFC
                     LFADDTGIFQPAQSLRSFVEERTAVDGSDLGARLAQLFQVLNTPEDKRSTKLDEQLAA
                     FAYVNGGLFQEALPLTDFDSAMRDALLDACALDWSAISPAIFGSLFQSIMDSAARRNL
                     GAHYTSEENILKLIKPLFLDELWAEFERVKGQRNRLFDFHKKLRTLTFFDPACGCGNF
                     LVVAYRELRLLELAVLRASQRLSGHAGQQSVDVHGLIGVDVDQFYGIEIEEFPAQIAQ
                     VALWLVDHQMNLRVSEEFACTLTAFRSRPARMWCTAMRSRSTGQSCCRLSAAAMCWAI
                     HRLWEKRSSQTRRRATLAR"
     gene            5892..6536
                     /locus_tag="PLB25_00860"
     CDS             5892..6536
                     /locus_tag="PLB25_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40177.1"
                     /translation="MLDVIQAVLAEIRAMEVKLKEIGAPDHLYTECVKQLRSGFSPTQ
                     LAAGWINHRDQMLKRATPLALQWAAWVLSRFDENKIDEEAMESLRISLAAQEKLLQET
                     ALPTGLREMLEHQTAGLRRALHLYKIQGIAPVQKVVSDSIGELVTAPQDLVAEVEAGS
                     PAVKQVFEQGKKMIGKAAELADKGSRIVKFSKEIYGLGMNGWHFGQQLISNMTQ"
     gene            complement(6545..6910)
                     /locus_tag="PLB25_00865"
     CDS             complement(6545..6910)
                     /locus_tag="PLB25_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40178.1"
                     /translation="MQLAKQASRNDAVRPNPPVQIGEVISDHILSTLITVSSLNPIQR
                     LKSLSRLPNVLPFRLVYRTPVLDDGGDFARQITFADAASGNFDQSLKSLVCHMNVRWR
                     MVVVSHTDDDAEEDGEGWH"
     gene            6825..7154
                     /locus_tag="PLB25_00870"
     CDS             6825..7154
                     /locus_tag="PLB25_00870"
                     /inference="COORDINATES: protein motif:HMM:NF021873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HOE40179.1"
                     /translation="MWSEITSPICTGGFGRTASLRDACLASCTARLAPSRASQLLGAN
                     RGVIPDHILNARLTAPFMLEIDFSDHTQGVFDADAYLASHAGPRLDELRDPAYFQRFF
                     VDAGALC"
     gene            7727..8680
                     /locus_tag="PLB25_00875"
     CDS             7727..8680
                     /locus_tag="PLB25_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013390834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOE40180.1"
                     /translation="MKLKLFAAAMVASVALVGTASAQQFFRIGTGGTAGTYYPVGGMI
                     ANAVSQPGKIIATAQASNGSLGNVNGIAGGAMEAGFSQSDVATWAQTGTGIFEGKPKV
                     AGLRMIANLYPESIHVVVKKGAGFKSIADLKGKRVALDEPGSGTLINARLVLAAYGIK
                     ESDIKPEYIKPNQAGDKMKDGALDAFFFTGGAPAGAIAELATAGGGIELLPIDGAAAD
                     SLRKSSSFFAVDSIPADTYKGVAAVKTLAVGAQLVTSDKVDANTVYQITKALYSDAAQ
                     KSLAAGHAKGKYITLQNAVQGVGIPYHPGAEKFYKEAGVLK"
     gene            8795..10999
                     /locus_tag="PLB25_00880"
     CDS             8795..10999
                     /locus_tag="PLB25_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HOE40181.1"
                     /translation="MSTIPITLPDDKQLLALEEKFDPEMRFRPSVPPGTVLIKWLLIT
                     LSVFHYYTAGFGLLRETTHRGVHLAFVLGLIFLVFSGSKATENRAVRHSLASPGGIPV
                     YDWLLGIGVAIAVLYIPYTFEDLAFRVGNPDTEDVVFGTVLFVALLEATRRSMGWPLP
                     VIALGFTFYSLFGQYFPGLLQHAGSSWSQFINHQYLTSQGIYGVAVGVVATYVFHFVL
                     FGVMATRIGLGQLFLDIASSIAGRYAGGPAKVSVFGSAMFGMLSGSSVANAVTVGSLT
                     IPAMIRVGYKREFAGAVEAAASTGGQITPPVLGAAAFLMIEFLGLPYQTIITAAIVPA
                     FMHFFGVFMQVHFEAKRYGLRGLTEEEMPKLRQSLKMRWPTLIPLFLLISILVSGRTP
                     YLAAFVGITSSAIVGVTTQVQGNTAKNWLLMVVMHLALMLVAFTNFDEMVKLGIFGVA
                     VAVTLVSMRLARIRGRIELSVLVEAFETGAKYALAVGAAAATVGIVIGVVTLTGVGFK
                     ISFIITGAATAIANALSMVVPSYLIAFQSLTLLAALVMTAAVCILMGAGIPTTANYII
                     MVTVAAPTLVQLGVEPLVAHFFVFYYGVLADITPPVALAAYAAAGMAGSDPFKTGNTA
                     FRLGIAKALVPFVFVFSPSLILVAKGFTWYDFTVTFVGCVLGITFLAMAFSKFFLVEM
                     KRWEQALCISAALLMVAPSLSATLIGVGMVVPVLLRQVKDWRLVQVARPLVR"
     gene            complement(11086..14055)
                     /locus_tag="PLB25_00885"
     CDS             complement(11086..14055)
                     /locus_tag="PLB25_00885"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE40182.1"
                     /translation="MTESDTFADLFAPEPESVAAPEGCAASAWKVLLVDDEKDIHAVL
                     NLALRDMRVEGKPLELLDAHSATEAKQVLADHPDISLILLDVVMESELAGLNLVRHIR
                     RDICNRMVQIVLVTGQPGYAPQREVVTDYEIDGYRLKSELTADKIFVSVYVALRTYKA
                     LRESELQRQQVESMAQVLSEKEQRLRSVVNTAPDAIIQANAQGTVIGWNEGASHLFGY
                     SSDEMLGQSINRLMPERLNHVHDLAMGRLRAGAPPHLLGKALELVGLRKDGSEFPIEL
                     VLGSWATVNGPHYSAVIRDISERKRSESGLRLAASVYANSYEGIIITDPDKTIVDVNP
                     AFTRITGYSRDEVLGRTPKLLSSGKHDAAFYKQMWSSIAEHDFWQGEICNRRSNAELY
                     TEILSLSVIRDSAGKLQHYIGVFSDISLLKAHEEELYRIAHFDMLTGVPNRRLLLDRL
                     SQALLRTRRTGRSVAVCYMDLDGFKLVNDRFGHDGGDKLLVEMTKRLRMVLRTDDTLG
                     RLGGDEFVLLLTDLADPQESQLALDRVLEIIKEPIEIAGQLVVVSASVGVTVYPADEA
                     DADVLLRHADQAMYLAKETGKNSYHYFDPLHDRQVQTQRHYMQRLREALDHDEFVLFF
                     QPKANLRNGDIVGFEALIRWQHPQRGLLAPAEFLHFMDGSELEVLVGEWVIDTVLRQL
                     ALWSRAGIDSRVSANVSADHLLRDNFAQRLRLILERYPEVAPERLELEILETAALADM
                     KRAVETITQCRNLGVQFALDDFGTGYSSLTYFLNLPVQLLKIDQSFVRMMLEDPGGLG
                     IVESVVRLAQAFNRPVIAEGVETLEHGSMLLYFGCYLAQGYGISRPMPADQVGPWLQA
                     WKADQSWRRMVTPAVDQDVTLQVAAQSHRAWVDKVTRQVTQLDGDSIIEADSSICSFG
                     RWYRGSGVTRYGSAPQYKDIAAHHERGHELAGMALTLAKSGKRREADLVLQELYESCD
                     SMVALLKKLISKPAIWR"
     gene            complement(14052..15824)
                     /locus_tag="PLB25_00890"
     CDS             complement(14052..15824)
                     /locus_tag="PLB25_00890"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE40183.1"
                     /translation="MAKLCIFGCRNFQMEVQACLAAEAWPDVVSRAFPARCGRPPVQW
                     EELRGLLPDDCSDVLILGRACLAALGDPPADFPATRVLVQQQCFHLLEGATWVDQAIA
                     DGDYLMSPGWLQNWRTHVANLGFADDTAAAFFQDFAKRLVLLDTGTDDKATGQLVQMQ
                     VVVGLPVHRIGVGLDHLRLFLHRLVLEWRLAQAQRLIKQQVKDHARELADHMTAMDLL
                     ARMTKSRTEPEVIGAIEDLFRMLFAPAVWYYISAEPTQGAGFHDVPAEIAEQVRALKA
                     AYGWTCSGEGFLLRVARDEHVFGHIAVDGLAFPQHRQRYLNLALAMTDMMALAIENAR
                     THKRLVEAEKMASLGVMVAGVAHEINTPVGVGVLAASTMQSRTQWLSQSFSERRMTQS
                     DLQGYLKDSQAQSSLILSNLERIGLLIDKFRQVAVNGLPQTKSRFRVKSCINEVIHSF
                     GDRMPPERIRVKLVCDDALELESYPGDWTSIFTNLISNSLQHGFAGRDLGCIQVQIAQ
                     LNAQLSIIYSDDGNGLTPEAQVRIFDPFFTTDMQNGIGLGMHLVYNLITQRLGGRIVV
                     EGSPGAGVCFHIEVPNHDGPREMQ"
     gene            complement(15849..16910)
                     /locus_tag="PLB25_00895"
     CDS             complement(15849..16910)
                     /locus_tag="PLB25_00895"
                     /inference="COORDINATES: protein motif:HMM:NF013381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HOE40184.1"
                     /translation="MTSATMTSMQRVLTTLGHQEPDQVPLFLLTTMHGAKELGMSIEQ
                     YFSKAEHVAQGQMLLLEKYRGDCVYPFFHASIETEAWGGTTLFSPEGPPVCGDPIITR
                     PTDIETLSSPRVADSPALQKVLATIRELKQQVADTVPIIGVAISPFSLPVMQMGFHSY
                     IELMHEQPTLFDRLMQLNEEFTVEWANAQLEAGATAICYFDPVSSTTSVPRGLYLKTG
                     QQVAKRTLARIKGPTATHLASGRGLSIVSDIADTGTAVVGVSALEDLAELKKAAAGRV
                     TLLGNLNGVEMRRWTPQQAELEVKRAIAKAGRGGGFVLADNHGEIPWQVPEAVLLAIR
                     DAVARWGRYPLTWAADWKD"
     gene            complement(16907..17539)
                     /locus_tag="PLB25_00900"
     CDS             complement(16907..17539)
                     /locus_tag="PLB25_00900"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOE40185.1"
                     /translation="METSVAPFREALESLDRIKADNIFQQVLVKRTPIEAVEQVVVPA
                     LEQIGAAWHAGEVALSQVYMSGRFCEELVDKTLPPSDPDRKHQPRSAIVVLSDYHDLG
                     KRMVYSVMRASGFELFDYGRMDVDALVKRAVSDKIRVLMISVLMLPSALKVREVCSAL
                     KKNGADVKVIVGGAPFLFDSELWKEVGADAMGSSASQAVTIVERMMGELQ"
     gene            complement(17690..>18151)
                     /locus_tag="PLB25_00905"
     CDS             complement(17690..>18151)
                     /locus_tag="PLB25_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOE40186.1"
                     /translation="RSLEKQSCRLRECLEQVVCSLGERLLAGRVVVQIDCAADLLAAG
                     STEDWSSIFSNLLGNSLKHGFKGREQGRIAVEVRHDAKGLRVSYSDDGIGLAAQSLVR
                     IFDPFFTTDLQHGMGLGMHLVYNLITHRLGGNITCASTHGCGVQVQIEVPS"
BASE COUNT         3723 a         5445 c         5225 g         3758 t
ORIGIN      
        1 tgaaaatccg ggtcgtcggc tcacccatct actctgacat gttcagtgcc atgggggcca
       61 acccgaccca aatgagctgg gccgatgcac aaccggcact ctccagcggg gccgtggacg
      121 gtcaggaaaa cccgttgttc ctgttcaccg tgctcaagat gcaaaacgtc ggtcagaagt
      181 ttctcaccgc ctggggctat gtggcagatc cgctgatttt tgtcgtcaac aaggacatct
      241 ggaactcatg gaccccggcc gaccaggctt tggtgcgtca ggcagcactt gatgccggca
      301 agcaggaaat tgtgctggcg cgcaagggcc tggtggaagc cgacaagccg ctgttcaaag
      361 acattgccgc catgggggtc acggtgactc agctcagccc ggccgagcgc gaagcctttg
      421 tcaaggtcac ccgtccggtt tatgagaagt ggaaagccac cgtcggtgcc gacctggtca
      481 aagcggctga gctggccatt gcagcgcgca aaaaataagc tggccaatct gccagcaccg
      541 accaggctgt ccgctgccgc gcctgcctga gccgttgaca cggctcaagg cgcacggtgc
      601 gggctgcttg ccggtgcctg cagcgcggac ttggtcggcg gtcattgaac cggcttgcct
      661 cggttgaccg cttgttttca tctgcaagcc gccatgcgca gttacttttt tggcttatct
      721 tggttgaaca cagattgaac caatgcaaat caggctgact tcagccatca atggtttccc
      781 catggcgcag caagcgccca caccaccaca atggatcaca tgactgccag ccgcccgccc
      841 aaaaaaccac gccatctgca agcctctgac ctgcgctgtg tcgctctgct ggccaccgaa
      901 gccacccagg cggtgaccgc catctcggaa ggggttcacc aatccatcct gggcacactt
      961 ggcgcaccca gtggggacac gcgggacaaa acctggggcc tgaccgggct gatctaccag
     1021 agcataaacg gtgtcaccca actgattggc aagggcgtgg ccagcgcgtt aacccggctg
     1081 gagccgctgc tgcagcgcct ggaaggtgaa gcggacgagt cagacgagcg cactgccgtg
     1141 atcgcggccc tgaacggggt catgggcgac cgcctgcaag ccagcagcaa cccgctggcc
     1201 acaccgatga cactgcgcca cgcaggcgca gtgcttgacc cgcagcaacc tgcccttgcc
     1261 agcgccagcg gcaaactgct gctggtcatc cacgggctgt gcatgaacga cttgcagtgg
     1321 accacccggc atgaggagca gaccgtcaac catgccgacg cactggccaa ggcgttggct
     1381 tgcacgccgg tctatttgcg ctacaacagc ggcttgcatg tgtcgcaaaa cggccatgcg
     1441 ctggccacgc tgctggagca attggtggcc aactggccgg tgccattgac tgaagtgagc
     1501 gtgctggtgc acagcatggg cggcctgatc acccgaagcg ccctgcacta cgccacacag
     1561 gctaacctgc agtggccgaa atacctgaaa aacatcgtct ttcttggcac accccatcat
     1621 ggtgcgccac tggagcgggc tggcaactgg gtcgatgtcg tgctgggcag cacgccctac
     1681 acccgtccgt ttgccaagct cgggcagttg cgcagtgccg gtgtgacaga tttgcgctat
     1741 ggccatgtgc tcgatgtcga ttggcagggc cacgaccggt ttcgccgcaa gcccgacagt
     1801 cgccagccgg taccgctgcc gcaagatgtt gcctgctttg ccgtggccgc aaccaccatg
     1861 gcgagccgca gcagcctggc agaccgactc atcggcgatg gcctggtgcc gctgcacagc
     1921 gccttgggcc aacacgatga agccgaccga caactggtgt ttgcgcccga gcgccagctc
     1981 atcgtctatg gcaccaatca catgcaactg ctcggcaagc ccgaggtgac ccggcagttg
     2041 ctggcctggc tgggctagtg cccgcaagcc cggccgattt ccgcacccat gacacctgac
     2101 catggggcgt aaactcactc gcctcaacaa cccgccgacg cgcccttttg ccatgaccac
     2161 actgcccgcc ctgccctgct acctcaacgg cgaattcacc accttgccga acgccaaaat
     2221 cagcgtgatg gaccggggtt ttatcttcgg cgacggtgtc tatgaagtgg tgccggtcta
     2281 tcaaggccac ctgttttgct ttgccaacca catggcccgg ctggagcgca gcctcaaaga
     2341 gctgcgcatg aacaacccgt atagccgccc ccaatggacc cagattgcta tgcggttaat
     2401 agctgactac gcacaatcca cgggggctac aggccaaaac attgatcaac tgatctacat
     2461 ccaggtgacc cgcggcgtgg ccatgcgcga ccacgtcatg cccaccgaca tcacgcccac
     2521 ggtatttgtc atgaccagca agatgacgac tcccagcagt gagcagcgcc gcctgggcgt
     2581 ggtctgcgtc actgcggatg acttccgctg ggaaaaggcg cacatcaaaa gcaccagcct
     2641 gcttggcgca gtgtttgccc ggcaaatcag ttttgaccag gaggcggttg aaaccatcat
     2701 gtttcgtgat ggttttctga gcgaggcagc agcctgcaac gtttgggtgg tcaaggacgg
     2761 ccggctgctg ggcacaccaa aagacaacct ggtgctggaa ggcattcgct atggtctgat
     2821 tgaagaaatc tgcgcaaagc agggcattgc cttcgagctt cggcgggtga cgcgtgccga
     2881 agtcatggct gccgacgaaa tcttgctgtc atcagccacc aaggaagtgc tgccggtcac
     2941 gctgctggac ggccagccgg tgggaaacgg cgcacccggc ccggtgtacg cgcagcttta
     3001 cgcgggttat caagaagcca aggccgagct gctgcaaacg cccgcatgat gcagggaacg
     3061 gccttgacgg cattgacttc aatgctgaca atgacagcgt tgaagtcaaa tcaggagtgt
     3121 tgcatggccg ttctgacgat tcgaaatgtg gatgacacga tcaagtccgc cttgcggata
     3181 caggccgccc agcgtggggt atccatggaa gaagaggctc gccgcatcct gcgcgaagca
     3241 ctgacgcgcg catcgccctc cacccccatg gggcagcgct tgttgcgccg ctttgccgac
     3301 gtggccagcg atgaattcgc cttgccgcaa cgccagccac cgcgcaccca agcgttcgaa
     3361 gattgaacac caatgcgcct gctcgatacc aacgtactgt ccgagttcat gcgacagcta
     3421 ccggcaccac aggtggtcgc ctggctggat gcgcagccca cgtcggccat gtttaccagt
     3481 gccatcagcc gtgccgaaat cgaattgggt ttggccctga tgcctgcagg caagcggcaa
     3541 ctggcgctgg cgcagtccgc gcgcgccatg ttcgacacag agttcgctga catgtgcctg
     3601 ccctttgacg agaccgcagc ggttcgctac gcgcaggtgg tgactgttcg cagccgcgcc
     3661 ggccacccca tcagtgtgga agatgcccag attgccgcca tcgccctggt gcacaagctg
     3721 gaactggtca cccgcaacgt agccgatttc accggcatcg aggggctaac cgtggttgat
     3781 ccctggcatc ttgacccctg aggcccacca cgacccgcgc ctgccatgcc cctgtcctgg
     3841 aacgaaatca aatcccgcgc gctgaacttc agccgcacct gggctgatgc tgcggatgaa
     3901 gccagcctgg ccaagccgtt ctggatcggc tttttcgaga tttttggcat caccgacaag
     3961 cgtcgccacc ttcgagctgg cggtcaaaaa gcacggtggc ggccagggtt ttgtcgattt
     4021 gttctggccc ggcatgctgc tggtggagca caaatcacgc gggcgcgacc tgaaccaggc
     4081 ttttgatcag gctctgggtt attttccggg tctgcctgaa cgcgacctgc cgcaactgat
     4141 cgtggtgtgc gactttgcgc gttttcgcgt ccacaagctg ccaagtggcg aagcgcttga
     4201 attcccgctg gccgagttgc accagaatgt cggccttttt ggcttgcttg cgagctacaa
     4261 gcagcaggtc atccgtccgc aagacccggt gaactttctg gctgccgaaa aaatgggccg
     4321 cctgcacgat gccctcaagg ccagcggtta tgggcaggaa gccgcgccgg gtgtcgcggc
     4381 gggccacgcg ctcgaagtct tgctggtgcg gctgctgttt tgcctgtttg ccgacgacac
     4441 cggtattttt caacccgcgc agtccttgcg cagttttgtc gaagagcgca ctgccgtcga
     4501 tggcagtgac ctgggcgcgc ggctggcaca gttgtttcag gtgctcaaca cgccggagga
     4561 caaacgcagc accaagcttg acgagcaact cgcggccttt gcgtatgtca atggcggact
     4621 gtttcaggag gccctgccgc tgaccgactt tgattcggcc atgcgtgacg cgctgctcga
     4681 tgcctgtgcg ctggactggt cggccatcag cccggcaatt tttggcagcc tgttccagag
     4741 cattatggac agcgccgccc ggcgcaacct gggcgcgcac tacacgtcag aagaaaacat
     4801 cctcaagctg atcaagccgc tgtttctgga cgaactgtgg gccgagtttg agcgcgtcaa
     4861 gggccagcgc aaccggctgt ttgactttca taaaaagctg cgcaccctga cattttttga
     4921 cccggcctgc ggctgtggca actttctggt ggtggcctac cgtgagctgc gcttgctgga
     4981 actggccgtg ttgcgcgcca gccagcgctt gagcggccat gccgggcagc agagtgtcga
     5041 tgtgcatgga ctgatcggtg tcgatgtgga ccagttctac ggcatcgaaa tcgaggaatt
     5101 cccggcgcag atcgcgcaag tggcactgtg gctggtggac caccagatga atctgcgtgt
     5161 cagcgaagag tttgcctgta ctttgaccgc attccgctca cgaccagccc gcatgtggtg
     5221 cacggcaatg cgctcacgct cgactgggca gagctgctgc cggttgagcg ctgcagctat
     5281 gtgctgggca atccaccgtt tgtgggaaaa aaggagcagt caaactcgca gaagagcgac
     5341 tttggcgcgg tgatgcacga cctgcacggt tctggggtac tggattttgt gacggcgtgg
     5401 tatgtcaaag cggcacggta tttgaaggaa aatcaggctc aagcctatat gcagcaagcg
     5461 atggaagcta tgaattcagg aattcatcgg gccagcgttg cgccaccacc ggccgggttg
     5521 gggtggccgg caggcgctca tgaaccttcg gtttttgaaa tcgcgcctgc cgaccacccc
     5581 aacccagcct cagcgcccgc cgctgcgcat caggcaaatg ggggacagaa cccaattccc
     5641 aatccaagct cagcgcaggc caatgagggt cggatttcaa tttccaactc cgcttcaatg
     5701 caggtcgcag gggatcagaa ccgaatttcc agcttaacca cagcgcaggt cgctgtggag
     5761 cggagcccaa tttccaactc gacgacagcg caggtcaatg agggtcggat cccaatttcg
     5821 ccccactcgg ccagccaagg cgacagcgcc cccgcgaaat tgggctctga cccccattgg
     5881 ccggaccatg cgtgcttgac gtgattcagg ccgtgttggc tgaaattcga gcaatggagg
     5941 taaaactcaa agaaatcggt gcccccgacc atttgtacac ggagtgtgtc aaacaactgc
     6001 gaagcgggtt ttcacccact caactagcag cgggatggat taatcataga gaccaaatgc
     6061 tcaagcgcgc gacaccactg gcactccaat gggcagcatg ggtgttgagt cggtttgatg
     6121 agaacaaaat tgacgaggaa gctatggaat ccttgcgaat cagcttggca gcgcaagaaa
     6181 agctcttgca ggaaaccgct ttgcctacag gtttgcgtga aatgctggaa catcagacag
     6241 cagggctacg ccgggcgctt cacctgtaca agatacaagg gattgcacca gtacagaagg
     6301 tcgtcagtga ttcaattggt gagctggtta ccgcccctca agacctcgtg gctgaggtgg
     6361 aagccggttc gcccgccgtc aaacaggttt ttgagcaggg aaagaaaatg attggcaagg
     6421 ctgcagaatt agctgacaag ggttccagga tcgtgaagtt cagtaaagaa atctatggac
     6481 tgggaatgaa cggctggcat ttcgggcagc agctaatttc caatatgact cagtaggaga
     6541 aatttcaatg ccaaccctct ccatcttctt cggcgtcatc gtccgtatgt gacacgacga
     6601 ccatccgcca ccgcacattc atgtggcata ccagggattt gaggctttgg tcaaaattgc
     6661 cactggcggc atcagcgaag gtgatttgcc tcgcaaagtc gccgccatcg tcaaggactg
     6721 gtgtccggta caccaagcgg aacggcaaga catttggcaa cctgctcaac gatttgagcc
     6781 gctggatcgg attcaaggag ctgaccgtga ttaaagtgct taggatgtgg tcagaaataa
     6841 cttcccctat ttgcaccggc ggatttgggc gcacagcgtc gttgcgagac gcttgtttag
     6901 ccagttgcac ggcgcgtctc gcgcctagcc gtgcatccca actccttggt gcaaaccggg
     6961 gtgttattcc cgaccacatc cttaacgccc gcctgactgc cccattcatg ctggagattg
     7021 acttctcgga tcacacacaa ggggtgtttg atgcggatgc ctacttggca agtcatgcgg
     7081 ggccgcgatt ggacgagctg cgagacccgg catatttcca gcgttttttt gttgatgcag
     7141 gcgcgctgtg ctgacccaac gggctggaga tttccccggc cagaatccgc gaactatcgg
     7201 tcgtccaact tgccgcctga gttcgcgcca ggccaggctg aagctgtgtt gggtggaagg
     7261 gttccgctgg agcatcaatc tacgccagcg cggcccaaag tgatcccagc gaatgggtgt
     7321 tgacggtgcc agtgaatcta gtcagcttga cgcagcacac cgtgcccctg ctgcaggcat
     7381 gctgggcctt gccaaccggc gcattgcatt ggcgcttggc gcttgacgag gcccttgcgc
     7441 tgcaggcgcg ccggcccggt ctgtctgacc gatttcgacc ggggctgcgc ctgtgggtgg
     7501 acatcaaatg cagccactga cttgcaacaa aataggcctc cagcccttat cctatatgcg
     7561 ctacaagcta tcatttttgt aataacgaaa cagcggcaaa ccggctttgg ccgactgcag
     7621 cgtcaagctg gcaggctgtc agcctgctga cagtgcagag tacactccca cagtcgttag
     7681 aaaccgcctg ttttcaggtt ttatcactca ccaccaggag attttcatga agctcaaact
     7741 ctttgccgct gccatggtgg catctgtggc actcgttggc accgccagcg cccagcagtt
     7801 tttccgcatt ggcaccggcg gcacggccgg cacttattac ccggtgggcg gcatgatcgc
     7861 caacgcggtg tcacaacccg gcaagatcat cgccaccgcg caggccagca atggttcact
     7921 gggcaatgtc aacggcattg ccggtggcgc gatggaagcg ggtttcagcc agtccgacgt
     7981 ggccacgtgg gcgcaaaccg gcactggcat ttttgagggc aagcccaagg tggctggcct
     8041 gcgcatgatt gccaacctgt accccgagag cattcacgtg gtggtgaaaa aaggcgcggg
     8101 tttcaagagc attgccgatc tcaagggcaa gcgcgtggca ctcgatgagc ccggttcggg
     8161 cacgctgatc aacgcccgcc tggtgctggc cgcttatggc atcaaagagt ccgacatcaa
     8221 acccgagtac atcaagccca accaggctgg cgacaagatg aaagacggcg cgcttgatgc
     8281 cttcttcttc accggcggtg cacctgctgg tgccattgcc gagttggcca ccgctggcgg
     8341 gggcattgag ttgctgccga ttgacggcgc tgccgctgac agcctgcgca aatccagcag
     8401 cttctttgcg gtggattcga tcccggctga cacctacaag ggcgtggctg cggtcaaaac
     8461 cctggcggtg ggcgcgcagt tggtcaccag cgacaaggtt gatgccaaca ccgtctatca
     8521 gatcaccaag gcgctgtaca gcgatgccgc tcagaagtcg ctcgctgccg gtcacgccaa
     8581 gggcaagtac atcacgctgc aaaacgccgt gcaaggtgtc ggcatcccct accacccagg
     8641 tgccgagaag ttctacaaag aagctggcgt gctgaaatag gccacgcttg actgtcccgc
     8701 cagacaccgc ctgacacagg ccctgatctg gcgcatggtt tgacgggcgg taccaccgcc
     8761 cgtttttgtt ggtaaacccc gaagccttcc acctatgagc accattccca tcaccttgcc
     8821 agacgacaaa caactgctgg cgctggagga aaagtttgac cccgagatgc gctttcgccc
     8881 ctcggtgcca ccgggcacgg tgctgatcaa gtggctgctg atcacgctga gcgtttttca
     8941 ttactacacg gctggttttg gcctgctgcg tgaaaccacg caccgtggtg tgcatctggc
     9001 gtttgtgctg ggcttgatct ttttggtgtt ttccggcagc aaggcaactg aaaaccgcgc
     9061 ggtgcgccac agtctggcct cacccggcgg cataccggtg tacgactggc tgctcggcat
     9121 tggtgtggca attgcagtgc tgtacatccc ctacaccttt gaagacctgg cttttcgtgt
     9181 cggcaacccc gacactgaag atgtggtgtt tggcacggtg ttgtttgtgg cgttgctgga
     9241 ggccacccgg cgcagcatgg gctggccgct gcccgtcatt gcgctgggct ttacttttta
     9301 ctcactgttt ggccagtatt ttccgggctt gctgcagcac gccggttcga gctggagcca
     9361 gttcatcaac caccagtacc tcaccagcca gggcatttat ggcgtggccg tgggcgtggt
     9421 ggccacctat gtgtttcatt ttgtgctgtt tggcgtgatg gccacgcgca tcgggctggg
     9481 gcaactgttt ctggacattg catcgagcat tgccgggcgc tacgccggtg gcccggccaa
     9541 ggtcagcgtg tttggttcgg ccatgtttgg catgttgtcg ggttcctcgg tggccaatgc
     9601 ggtcaccgtg gggtcgctga ccattccagc gatgattcgc gtcggctaca agcgtgaatt
     9661 tgccggtgcc gttgaagcgg ccgcctctac cggcgggcag atcacgccac cggtgctggg
     9721 cgcggcggcg tttttgatga tcgaattttt gggtttgccg tatcagacca tcatcaccgc
     9781 cgcgatcgtg ccggctttca tgcacttttt tggcgtattc atgcaggtgc actttgaggc
     9841 caagcgctac ggtttgcgtg gcctgaccga ggaagaaatg cccaagctgc ggcaaagcct
     9901 gaagatgcgc tggccaacgc tgattccgct gtttttgctg atcagcattc tggtcagcgg
     9961 gcgcacgccg tatctggcgg cgtttgtcgg catcacgtcg agtgccattg tgggcgtcac
    10021 cacccaggtg cagggcaaca ccgccaaaaa ttggctgctg atggtggtga tgcacctggc
    10081 gctgatgttg gtggccttta ccaactttga cgagatggtc aaactgggca tctttggtgt
    10141 ggcggtggcc gtcacactgg tcagcatgcg gctggcgcgc attcggggtc gcattgaact
    10201 atcggtgctg gtggaggcgt ttgaaaccgg tgccaaatac gcgctggccg tgggtgccgc
    10261 agccgccacg gtgggcattg tgattggtgt ggtcacgctc accggggtgg gcttcaagat
    10321 cagcttcatc atcaccggtg ccgccacagc aatagccaac gcgctgagca tggtcgtgcc
    10381 gagctatctg atcgccttcc agtcgctcac gctgctggcg gcgctggtga tgacggcggc
    10441 ggtgtgcatt ttgatgggcg cgggcattcc caccaccgcc aactacatca tcatggtgac
    10501 ggtggcagct cccacgctgg tgcaactggg ggtggagccg ctggtggcgc acttttttgt
    10561 gttttattac ggtgtgctgg ccgacatcac gccacccgtg gcgctggccg cctacgcggc
    10621 agcaggcatg gcgggaagcg acccgttcaa gactggcaac accgcgtttc ggctcggcat
    10681 tgccaaggcc ctggtgccct ttgtgtttgt cttttcaccc tcgttgatcc tggtggccaa
    10741 gggctttacc tggtatgact ttaccgtcac ctttgtcggc tgcgtgctgg gcatcacctt
    10801 tttggcgatg gccttctcca agtttttcct ggttgaaatg aagcgctggg agcaagcgct
    10861 gtgcatcagc gccgcactgt tgatggtggc cccgagcctg agcgccacgc tgattggcgt
    10921 gggcatggtg gtgccggtgc tgctgcggca ggtgaaggac tggcgactgg tgcaggtggc
    10981 tcggcccttg gtgcgctgag ttgcgggcgc caatgttcat cctcggggtt tgtcgatgtc
    11041 gttgaccttg aggggtcgag cgaaaactca tttttgcctg accaattagc gccagatggc
    11101 tggcttggaa atcagttttt tgagcaaagc caccatgctg tcgcaggatt cgtaaagctc
    11161 ctgcaagacc agatcggcct cgcgacgctt gcctgacttg gccagggtca aggccatgcc
    11221 agccagctca tgcccgcgtt cgtgatgcgc tgcaatgtcc ttgtactgcg gggcgctgcc
    11281 ataacgggtc acaccgctgc ctcggtacca gcgtccaaaa ctgcagatac tgctatcggc
    11341 ctcaatgatt gagtcgccat cgagttgtgt tacttgccgg gtcactttgt ccacccaggc
    11401 tcgatggctt tgggcggcca cctgcaaggt gacatcctga tccacggctg gggtaaccat
    11461 acgccgccag gactgatctg ctttccatgc ctgcaaccag gggccaacct gatctgctgg
    11521 cataggccgg gagatgccat aaccttgcgc caaatagcag ccaaaataca gcagcatgga
    11581 accatgttcc agggtctcaa cgccttcggc gatcaccggg cggttaaagg cctgcgccaa
    11641 acgcaccaca ctctccacaa tgcccaggcc gcccgggtct tcgagcatca tgcgcacaaa
    11701 actctggtca atcttgagca gttgcaccgg caggttgaga aaataggtca gcgaggaata
    11761 gcctgtacca aagtcatcca gcgcaaactg caccccgagg ttgcggcact gggtgatggt
    11821 ttcgaccgct cttttcatat cggcaagagc cgctgtttca agaatttcaa gctccagccg
    11881 ctccggtgcc acttcagggt agcgctccag gatcaggcgc aaccgctgcg caaaattgtc
    11941 acgcagcaga tgatctgcac tgacgttggc actcaccctg ctgtcaattc cagctctgct
    12001 ccaaagcgcc aattgcctga gcacggtgtc aataacccat tcgcccacca gcacttcgag
    12061 ttcgctgcca tccatgaagt gcagaaactc ggcaggagca agcaagcctc tttgcggatg
    12121 ctgccaacga atcagcgcct caaagcccac gatatcgccg ttgcgcagat ttgccttggg
    12181 ctgaaagaac agcacaaact cgtcatggtc caatgcctcg cgcaatcgtt gcatgtaatg
    12241 ccgctgcgtc tggacttgac ggtcgtgcag cgggtcaaag taatggtagc tgttcttgcc
    12301 agtctccttg gccaggtaca tggcctggtc ggcgtgacgc agcaagacat cggcatcagc
    12361 ttcgtcggcc ggataaaccg tcacaccgac actggccgac accaccacca actgcccggc
    12421 tatttctatt ggctccttga tgatttccag tacccggtcc agcgccaact gactttcttg
    12481 cgggtcagcc agatcggtca gcagcagaac aaactcgtca ccgccgagtc gccccaaggt
    12541 atcgtccgtt cgcaagacca ttctcagacg tttggtcatt tccaccagca gtttgtcgcc
    12601 gccatcatgt ccaaaccggt cattgacaag cttgaaccca tccagatcca tgtaacagac
    12661 ggccaccgac cgaccggttc gccgggtgcg caaaagtgct tgactcaaac ggtccagcag
    12721 aagtcggcga ttgggaaccc cggtcagcat gtcaaagtgc gcgatgcgat acaactcttc
    12781 ctcgtgcgcc ttgagcaggc tgatgtccga gaagacgccg atgtaatgct gcaacttgcc
    12841 tgcactgtcc cgaatcactg aaagagacag tatctcggtg tacaactcgg cgttgctgcg
    12901 ccggttgcag atctcacctt gccaaaagtc atgctcggcg atggaagacc acatctgctt
    12961 gtagaaagcg gcgtcgtgct tgccagacga tagcagtttg ggtgtccgtc caagcacttc
    13021 atcacgcgaa tagcctgtga tgcgcgtgaa agccgggttc acatccacaa tggtcttgtc
    13081 gggatcggta atgatgatgc cttcgtaact gttggcataa acgctggcgg caagacgcag
    13141 ccctgactcg ctgcgctttc tctcgctgat gtcccgaatg acagcactgt agtgcgggcc
    13201 attcacagtc gcccatgaac ccagcaccag ctcgatggga aattcactgc catcctttcg
    13261 cagcccgacc aactcaagcg ccttgcccag caagtggggt ggcgcaccag cacgcaagcg
    13321 gcccatggcc aggtcgtgaa catggttgag ccgttcaggc atcaatcggt tgatcgactg
    13381 gcccagcatc tcgtcactgc tgtacccgaa cagatggctg gcaccctcat tccacccgat
    13441 caccgtacct tgcgcgttgg cctgaatgat ggcatctggt gccgtattaa ccacagaacg
    13501 caggcgttgc tctttctcgc tgagcacctg cgccatcgac tcgacctgct ggcgctgcaa
    13561 ttcagattcg cgcagcgcct tgtaggtgcg cagcgcgacg tataccgaga caaagatctt
    13621 gtcggcagtc agctcggatt tcaggcgata gccgtcaatc tcatagtctg tcaccacctc
    13681 gcgctgcggc gcatagccgg gctgcccggt gaccagaaca atctgaacca tgcggttgca
    13741 gatgtcacga cgaatatggc gcaccagatt caggccagcc agctcggact ccatcaccac
    13801 atccagcaat atcaaagaga tgtccggatg gtctgccaat acttgtttgg cctcagtagc
    13861 cgagtgtgcg tccagcaact caagcggctt accttcgacc cgcatgtccc tgagtgccag
    13921 gttcaggacg gcatggatat ctttttcgtc gtccaccagc agcactttcc aggccgatgc
    13981 agcgcaacct tcgggtgccg ctacagactc tggttcaggt gcaaacaggt cggcaaaggt
    14041 gtcagattca gtcattgcat ttctcttgga ccatcgtgat tggggacctc aatgtgaaaa
    14101 cacacccccg caccggggct gccctcaacg actatacgtc cacccaggcg ctgggtgatc
    14161 aggttataga cgaggtgcat gcccaaaccg atgccatttt gcatgtcggt ggtaaaaaat
    14221 gggtcaaaaa tgcgcacttg cgcttcaggc gtgagtccat tgccgtcgtc cgagtaaatg
    14281 atggaaagct gggcgttcaa ttgcgcaatt tgcacttgaa tacaccccag gtcacgcccc
    14341 gcgaagccgt gctgcagcga gttggatatc aggttggtga atatgctggt ccagtcacct
    14401 gggtaactct caagctccaa agcatcgtcg caaaccagct tgacccggat acgttcgggt
    14461 ggcatgcgat caccaaaact gtggatcacc tcgttgatgc aacttttgac ccgaaagcga
    14521 cttttcgtct gcggcaaacc attgacagcc acctggcgaa acttgtcaat caacagcccg
    14581 atgcgctcca gattggaaag gatcaacgac gactgggcct gcgaatcctt cagatagcct
    14641 tgcaggtctg attgcgtcat gcgccgctct gaaaagcttt gcgacagcca ctgggtgcgc
    14701 gactgcatgg tggacgcagc cagcaccccg acacccactg gcgtgttgat ttcgtgggcc
    14761 acaccggcca ccatcacccc cagcgaggcc attttttcgg cttcgaccag ccgcttgtgg
    14821 gttctggcgt tttcaatcgc cagcgccatc atgtcggtca tggccagggc cagattgagg
    14881 tagcgctgac ggtgttgggg gaaggccagg ccatccactg caatatggcc aaacacatgc
    14941 tcatcgcgcg ccacccgcag caaaaatccc tcgccagaac aggtccaccc ataagccgcc
    15001 ttcaatgccc gaacctgctc tgcgatctcg gcgggcacat catgaaagcc tgccccttgc
    15061 gttggttcgg ctgaaatgta gtaccagacg gctggcgcaa acagcatgcg aaacaggtct
    15121 tcaatggcac cgatgacctc aggttcggtg cgagatttgg tcatccgtgc caacaggtcc
    15181 atcgcggtca tgtggtcagc cagttcgcgc gcgtgatctt tgacttgctg ctttatcagc
    15241 ctctgggctt gagcgagccg ccattccagc accagtcgat gcaggaacaa gcgcagatga
    15301 tcgagcccaa cccctatgcg gtgaaccggc aaaccgacga ccacctgcat ctgcaccagt
    15361 tgaccagtcg ctttgtcgtc ggtgccggtg tcgagcaaaa ccagacgctt tgcaaagtcc
    15421 tgaaaaaatg ctgctgcggt gtcgtcggca aagcccaggt tggccacatg tgttcgccag
    15481 ttttgcagcc agcctggact catcaggtag tcaccatcgg cgatcgcctg atccacccat
    15541 gtggcaccct ctagcagatg gaaacactgc tgctgaacca gtacccgtgt ggccggaaag
    15601 tctgcgggcg ggtcgcccag cgcggcaagg caggcacgac ccaaaatcag cacatccgaa
    15661 caatcgtcgg gcagaagacc gcgaagctct tcccactgca ccggtggtcg cccacaacgt
    15721 gcggggaaag cccgcgaaac gacatccggc caagcctcag ccgcaaggca ggcttgcacc
    15781 tccatctgaa aattccggca tccaaagata cagagcttgg ccataacacg ctcactggtg
    15841 agtggcggct agtctttcca gtctgctgcc caggtcagtg gataccggcc ccagcgtgcc
    15901 accgcatcgc ggatggccag cagcacagcc tcaggcactt gccaggggat ttcgccatgg
    15961 ttgtcggcca gcacaaaccc gccaccacgc ccggcttttg caattgcacg cttgacttcc
    16021 agctcggcct gctgcggtgt ccagcgccgc atttccacgc cattgagatt gcccagcagc
    16081 gtgacccgcc cagcagccgc ttttttgagt tcagccagat cttcgagggc actgacccca
    16141 accactgcag tgccggtgtc agcaatatcc gacacgatgg aaagaccacg cccggaggcc
    16201 agatgggtgg cggtggggcc tttgattctg gccagcgtac gctttgccac ctgttggccg
    16261 gtcttgagat acagcccgcg tggaacgctg gtggtagacg acaccgggtc aaagtaacaa
    16321 atcgccgtgg cacccgcttc caactgggcg ttggcccatt ccacggtgaa ttcctcattg
    16381 agttgcatca gcctgtcaaa caaggtgggc tgttcgtgca tcagctcaat ataagaatga
    16441 aagcccattt gcatgaccgg cagcgagaac ggcgagatcg ccacgccaat gatgggcacg
    16501 gtgtctgcca cctgttgttt gagctcgcga atggtggcca gaaccttttg caacgctggc
    16561 gagtccgcaa ccctgggtga agaaagggtt tctatgtccg tgggtcgggt gatgatgggg
    16621 tcaccacata ccgggggacc ctctggcgag aacagggtgg ttccgcccca ggcctcggtt
    16681 tcaatcgacg catgaaaaaa cggatagaca caatcgcccc ggtatttttc aagcaataac
    16741 atttgccctt gcgccacatg ttcggctttg gaaaagtact gttcgatgga catcccgagt
    16801 tctttggcac cgtgcatggt tgtcagcaga aacaagggta cctggtcagg ctcctggtga
    16861 cccaaggtgg tcagaacccg ctgcatggag gtcatggttg cagatgtcat tgcagttctc
    16921 ccatcatgcg ctcaactatc gttaccgcct gcgacgcgct gctgcccatg gcatcggcac
    16981 caacttcttt ccagagttcg ctgtcgaaca aaaaaggcgc accgcccaca atcaccttga
    17041 catcagcgcc attttttttc agcgctgaac acacttcgcg caccttcagg gccgacggca
    17101 gcatcaaaac agagatcatc agcacccgaa tcttgtccga gacagcgcgc ttgaccagcg
    17161 cgtcgacatc catgcgcccg tagtcaaaca actcaaaacc actggcgcgc atgaccgaat
    17221 acaccattct tttgccaaga tcgtggtagt cgctcagcac cacgatggct gaacggggct
    17281 gatgtttgcg atccgggtca ctcggtggca gcgtcttgtc aaccagttct tcgcagaagc
    17341 gtccactcat gtagacctgc gacaaagcca cctcaccggc atgccaggcg gcaccgattt
    17401 gctcaagtgc gggcaccacc acctgctcga ccgcctcaat cggtgttcgt ttcacaagca
    17461 cctgctgaaa gatgttgtct gccttgatcc gatccaaaga ttccaatgct tcgcgaaagg
    17521 gtgcaactga tgtttccatt tgggcaatct ccttgatcca tatgcggctg gatacttttc
    17581 atcgtagcca ccggccagca gaaagccaat cgggtcaata cctgaggcgc tcaggtaaaa
    17641 cacctgatcg tcaggctggc gcaccgcccg gagaacgcgt cgggcgtgtt caggaaggca
    17701 cctcaatctg cacctgcacg ccgcatccgt gcgtgctcgc gcaggtgatg ttgccaccaa
    17761 ggcggtgggt aatgaggttg tacacaagat gcatcccgag tcccatgcca tgctggaggt
    17821 cggtggtgaa aaaggggtcg aagatgcgca ccagtgactg ggctgccagc ccaatgccgt
    17881 catcgctgta actgacccgc aagccctttg cgtcgtgcct gacttcgaca gctatccgac
    17941 cctgctcgcg ccccttgaac ccatgcttga gggagttgcc aagcaggttg ctgaagatgc
    18001 ttgaccagtc ctcggtgctg cccgctgcaa gcaaatctgc tgcgcagtcg atctgtacca
    18061 cgacgcggcc tgccagcaag cgctcaccca ggctgcaaac cacctgctcc aggcattccc
    18121 gcagccgaca cgactgcttt tcaagcgacc t
//
LOCUS       DAOSLL010000011        17711 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_3403_length_17711_cov_4.413344, whole genome shotgun sequence.
ACCESSION   DAOSLL010000011 DAOSLL010000000
VERSION     DAOSLL010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 17711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17711
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3403_length_17711_cov_4.413344"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..458)
                     /locus_tag="PLB25_00910"
     CDS             complement(54..458)
                     /locus_tag="PLB25_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40187.1"
                     /translation="MKKVLSLTFEDGSPVPSFQSLMDTLQTMMRNCCRTPGGAPDAAT
                     FEIITTPNHIPKRALETITQIKPQTARGTKINAGFNQETRFFWGGELQLKPRHGKVID
                     RFRDWIGNGIFNPAAGRKPTGRHGEHVTFQSR"
     gene            complement(634..861)
                     /locus_tag="PLB25_00915"
     CDS             complement(634..861)
                     /locus_tag="PLB25_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40188.1"
                     /translation="MVKLGVNRDRKQGISQVNYGLLTDDRGCPVAVPVHAGNTSDGTT
                     FMPEETRLREEFGIRRLGVGGESGHDLANGR"
     gene            complement(941..1075)
                     /locus_tag="PLB25_00920"
                     /pseudo
     CDS             complement(941..1075)
                     /locus_tag="PLB25_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105979.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1317..2039)
                     /locus_tag="PLB25_00925"
     CDS             complement(1317..2039)
                     /locus_tag="PLB25_00925"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE40189.1"
                     /translation="MTANFMPALLDLASQPFLASGRFAYHYARGKLGGDCIFRELLKR
                     GIFPPQGHFLDLGCGQGSLFAWLLAARRLYEQGQWPADWPAAPQPLSLRGVELMQNDV
                     DRAALAFAAKHPLVRIEQGDMNRVDFGRPDVITILDALHYFDHGQQQRVLQRIHAALP
                     PGGLFLTRIGDASAGLPFHVSNWVDHVVTFLRGHRLSRLYCRSLGDWQQLLHDIGFDV
                     QSNTMTEGKPFANVMLVCRVRV"
     gene            complement(2036..2836)
                     /locus_tag="PLB25_00930"
     CDS             complement(2036..2836)
                     /locus_tag="PLB25_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008348288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOE40190.1"
                     /translation="MTRRWTSTPILKATAAVHAAAALSTLAVPTAWPWTLAAVLGNHA
                     ALTLAGLLPRSTLLGPNLTRLPTTALTHHQVALTLDDGPDPQVTPRVLDLLDTGAAKA
                     NFFCIGWRARQHPALCREIVARGHRVENHGDAHSNAFSLFGPARMHADISAAQATLSD
                     ITGQAPLFFRPTAGLRNPFLQPVLTQLELRLVSWTRRAYDTREGRAQRVLQRLSHGLA
                     AGDILLMHDGHGARAPDGQPVILTALPLLLQAFALQGLQPVTLKDALT"
     gene            complement(2833..3525)
                     /locus_tag="PLB25_00935"
     CDS             complement(2833..3525)
                     /locus_tag="PLB25_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40191.1"
                     /translation="MASRAGGYFCLFPQAEVLVSSAFYRCGANTLLVMLPGALMTPQH
                     TLQAGLFEAVAQRDLALDLIVPDLHALEGCNRAALHTLEADILAPARALYQHVWLGGI
                     SRGGQLALSCLAEQTAQVDGLCLLAPYAGSRLTTNAIRRGGGIDAWQASAEQLQDPEF
                     RLWQWLKRPDIQGPVFMGYGVQDRFADGMAWLAERLPTASHCTLPGGHDWAVWRQLWG
                     HFLDLGFFARLP"
     gene            complement(3540..5894)
                     /locus_tag="PLB25_00940"
     CDS             complement(3540..5894)
                     /locus_tag="PLB25_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HOE40192.1"
                     /translation="MKSLKLRIFLLWLLCMLAGSVVVWNSRFSADMSFFLPSKPSAEQ
                     RALVGQLQDGTVSRLLMLSIEGADAAQRASVSRELRQRLANNPAYVSVQNGEAGGLEG
                     ERDVLLRYRYQLSPSVSPDRFTEAGLRAAVANTIDLLSSPAGLMFKPYLARDPTGELL
                     EVIAQFNPGAQPETRAGVWASRNGERAMLLAQTRAQGSDTDGQAAVIADLQQVFAQVL
                     SAQHRSGMTLQISGPGKFAVQARETIQAEVSRLFFVSMAGIVGVLLWVYRSPRLLALG
                     LLPMLSGALAGVAVVSLVHGTVFGITVGFGSALIGEAVDYAIYFFVQSGRMGLDNWRR
                     VFWPTVRLGVLTSALGFGALLFSGFPGLSQLGLYALSGVVTAALVTRFVLPTLAGAQF
                     CVPAPRPWVHRVLTGLVHAHRLRALVGLLALLAGGYLWQQRASLWDANLSALSTVSQA
                     EAAADSRMRADLAAPDARYLVTIGAADQESALQAAERAGAQLDRLVEQGVIGGYDSPA
                     RFLPSTLLQTRRLVSLPVADVLQTRLRAALIDSPLGADKLAPFVADVQAARSAGVMRR
                     ADLNGSALALVVDSMLMPVHSGGWTVMMPLRPAANAPDADIPPEKLRTALAGSGALFV
                     DLKAEFDALYSGYMDQAVRLSLAGFLAIVVLLALTLRSRARLLRVLLALVLTVLGVIA
                     LLHLAGVRLHLLHLVGMLLIVAVGSNYALFFDRAAGGEPLDAATLLSIAVATLTTAIG
                     FGALALSSVPVLQAIGVTVGPGAVLALLLSAVLVYPAGGRCPQK"
     gene            complement(5891..6460)
                     /locus_tag="PLB25_00945"
     CDS             complement(5891..6460)
                     /locus_tag="PLB25_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HOE40193.1"
                     /translation="MIRRFGLILLLLGFATSAQAAFDIEQLMAELTQHKGGRARFVEK
                     RHIALLDKPVQSSGEMLYNPPDRLEKRTLLPKAETMVLDKDQLSLERDKRKLSINLSS
                     RPEAIAFVDSIRSTLSGNRRTLERNYTLNLQGESKQWVLTLVPIEPAISALLQRITIN
                     GSKSQVRYIEYLQVDGDRSEITIEPIDNQ"
     gene            complement(6457..7362)
                     /locus_tag="PLB25_00950"
     CDS             complement(6457..7362)
                     /locus_tag="PLB25_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014252228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HOE40194.1"
                     /translation="MNPDTRPDWMQQQERSQALVLRLMVWISLTFGRRLGRLVLRGIA
                     TYFVLFAPMARRASRAYLTHALGRHAGWRDGYRHVFSFASTIHDRIYLLNDRFDEFDI
                     QVLGAPALLAALQKQAGALLVGAHLGSFEVLRAMGRGQHGLQVAMLMYEQNARKLNAT
                     LAAINPRAMQDIIALGRLESMLTVRDKLDAGYLVGMLADRSLGGDATLAADFLGHSAN
                     FPVGPWRMAVMLKRPVFLMAGLYLGGNRYQLHFELLADFSAVERGQRDGAIQAAVQRY
                     ADSLSRLCQLAPYNWFNFFDFWQKK"
     gene            complement(7359..7664)
                     /locus_tag="PLB25_00955"
     CDS             complement(7359..7664)
                     /locus_tag="PLB25_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40195.1"
                     /translation="MADAEFTWHVPVHHPAFAGHFPGQPIVPGVVLLDLALLFAQQHL
                     DQFASGWQVRQAKFLSPCGPGDELVFAFSGGSGGVLTFTVRSAGRTMASGSLLPPHR"
     gene            complement(7657..8991)
                     /locus_tag="PLB25_00960"
     CDS             complement(7657..8991)
                     /locus_tag="PLB25_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE40196.1"
                     /translation="MSALPIVNGHQLDDTLAWCPAGPVTVRAFLADAQAVAKLLPKGN
                     WVLNLCEDRYHFAVLFAACLLADKTSLQPASQSGETLLRLAQDYPGVFCVTDGAFELP
                     GLPRLAFPRLATLLRPAVTDMPMIAASRTVAILFTSGSTGLPQPHGKTWGKLVQNGQA
                     EAAALALGSHPPALVGTVPAQHSYGFESTFLLALHGGCSFWSGKPFYPQDVVDALKAV
                     PRPRMLVTTPFHLAVLLGSGLDWPALDLCLSATAPLSAELAAQVERQSGAPVHEIYGS
                     TESSQLACRRTTDGPLWRLLPGVQLTQTHDTTYALGGHVQGRVPLSDIIELQSDGQFL
                     LHGRHADLVNIAGKRTSLAYLNHQLCAIPGVQDGVFYLPDSNAAERNTRLCAFVVAPS
                     MQRAELMAALRGRIDPIFLPRPLVFVESLPRISTGKLPRSALQALYQKQTHG"
     gene            complement(8988..9668)
                     /locus_tag="PLB25_00965"
     CDS             complement(8988..9668)
                     /locus_tag="PLB25_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40197.1"
                     /translation="MSLSWSQAWRGAAAGVLLLAWAVAAHLGSADIGSADLNAAIATT
                     PLLLAWTALLWQFKPRWVRLTGFALALSAVLALWPQLRQSIALLYYLQHLGTHLALAV
                     LFGRTLHGSGEPLITRMARSIYDHALSERKVRYTRQVTLAWTLFFLGNALVSTGLFLW
                     AAPTVWSVHANLLTGPLIAGMFLAEHLVRRFVLPPDERPGLSDVVRAYRQRGAMGGAA
                     RQSPESGA"
     gene            complement(9665..9946)
                     /locus_tag="PLB25_00970"
     CDS             complement(9665..9946)
                     /locus_tag="PLB25_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HOE40198.1"
                     /translation="MSDVPSVSHDALMTEVAALIIEALNLEVLVSDISPDGPLYGEGL
                     GLDSIDMLEISLVISKHYGFQLRSDNEDNAQIYASLRALTHYIASQRTK"
     gene            10138..10785
                     /locus_tag="PLB25_00975"
     CDS             10138..10785
                     /locus_tag="PLB25_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HOE40199.1"
                     /translation="MKSQWPQRIKDRLVPYWPLKAFGTTLFMALFFWAYFQVIHHQYG
                     LPMVMPRIWLDDWIGFTPYSFPVYASLWVYVSLPPALMGNLRELILYGLWVALMCLFC
                     LALFWLFPTLTPTVDMDWRNYPAMVMIKGVDAAGNACPSLHVASAVFSACWLGRILGQ
                     LHTPKTVTWLSWAICLAIAWSTMATLQHVALDVLCGVAVGLGFAVLSLRQIKNTA"
     gene            complement(10827..11570)
                     /locus_tag="PLB25_00980"
     CDS             complement(10827..11570)
                     /locus_tag="PLB25_00980"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE40200.1"
                     /translation="MARARQIGLFYVMLLWLGMMLLIGNVAVLPLLLTPRWFREPLVQ
                     GLISHLFRVFLAGAQRSGLMQLDLNQLDVLNHHKGLVLVANHPSMIDVFLVISRVRRA
                     ACLMKASIGSNILFGAGAYLAGYVSNRKPGEMLRSASNTVRRGPHLLVFPEGTRTTRQ
                     PVNDIKPGVALIAKRTGQPLQTILIRTNSPYLSKGWRLFRPPQFPLVYQASLGAQLSA
                     TGVCAHTARSLQDYFEAQMACSIDPTFRL"
     gene            complement(11578..12579)
                     /locus_tag="PLB25_00985"
     CDS             complement(11578..12579)
                     /locus_tag="PLB25_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40201.1"
                     /translation="MLVHHGQTAGEPAIAIPDGLFLGSLCTATGEAAAVGSALRLHCQ
                     VPIPCLGGEPYAQQAWFVDGASDTCTSMVQDERGVTVAHTEDFVFATISSEHGALEQQ
                     SRLVYEALLSRLRQLNKPHLLRLWNYVPRINEIDGGIERYQLFNFGRRQAFDSMGYTV
                     GDGAPAACALGTRGAALIVAALASKHPVIAIENPRQISAYHYPRQYGSHPPIFSRAAW
                     LAQKSGPDLLFVSGTASIVGHRTLHAGNVRAQVQESVTNIEAVLHGANQRAGAHLWTL
                     GGLQGRVYIRHAADFPLIQAYLLTRGMTQFGFVQADICRSDLLLEIEAEGQTFCAHQ"
     gene            complement(12787..13390)
                     /locus_tag="PLB25_00990"
                     /pseudo
     CDS             complement(12787..13390)
                     /locus_tag="PLB25_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011486255.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            13539..14885
                     /locus_tag="PLB25_00995"
     CDS             13539..14885
                     /locus_tag="PLB25_00995"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOE40202.1"
                     /translation="MQNTTIKPPPNATPLVCDVLVIGGGPAGSTVSSLLAEQGHKVVV
                     LEKSHHPRFHIGESLLPANLPLFEAMGIADEVKAIGMFKPGAEFVSPTHNAKQTFHFA
                     DAWNKSMPHAYQVKRAEFDEILIRHAARKGAEVHEGCKVTAVDFLADGGACITARHDD
                     ERVQQWQARFVVDASGRDTFFANRFKIKKSNPKHNSSAVYAHFSQAQRNEGQDEGNIT
                     IFWFDHGWFWFIPLTNGTTSVGMVTWPYHIKTRGQRSLEQFLMDNIATSPTLSARLQN
                     ATRVSPVEATGNFSYVSERNHGANYLMLGDAYAFIDPVFSSGVWLAMNSAVIGAEAIS
                     SCLKNPAKSAVALARFDRLMRHGPREFSWFIYRVTNPIMRDFFMHPKNVMRVKEALLS
                     VLAGDIFGKTPIWRSILAFKMLYYSANILQPRRAFRAWQQRRFNIRKVDDAVVYNA"
     gene            14917..15690
                     /locus_tag="PLB25_01000"
     CDS             14917..15690
                     /locus_tag="PLB25_01000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE40203.1"
                     /translation="MSEPLCTASTSHLVLIPSYNPGPNVLDTVRQARRFWHPVWVVVD
                     GSTDGTGLQLQALAQTDAGLRVIVLPHNQGKGAAVLHGITLAAKAGFTHVLTMDSDGQ
                     HPAEAIPAFMAASRHEPAGMVLGKPVFGPEAPTLRVNGRKVSNGWANLETLWMGIGDS
                     LYGFRVYPIAPLLQVMQGNRFMRRFDFDPEAVVRLCWAGVHPINIDAPVRYLSAQEGG
                     VSHFNYLRDNVLLTWMHTRLLLGFLLRLPLLIWRRATKN"
     gene            15692..16450
                     /locus_tag="PLB25_01005"
     CDS             15692..16450
                     /locus_tag="PLB25_01005"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE40204.1"
                     /translation="MNKPVARTSALKSDHRMDPYFTTEEGRRSLTQAMFNQAAPGYDN
                     AESLTALGSGVWYRREVLLRNGLAAGMTLLDVAAGTGLVTVPGHAIVGPQGRVLALDP
                     SPGMLAELRKKIQVETIEAFAESIPLPDGQIDFISMGYALRHVGDLNLAFAEYFRVLC
                     PGGRVCIMEISRPRSALARALLGFHIKMVVPLLARLTRRHADIQRLWEYYGDTIEAAL
                     EPELILDALRRAGFTDVACCVTLGIFREYTGRRP"
     gene            16447..17664
                     /locus_tag="PLB25_01010"
     CDS             16447..17664
                     /locus_tag="PLB25_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOE40205.1"
                     /translation="MNTTEVFLIAMLIIFSIPWLIWRLGNTDYFAPLVVVQILAGIVL
                     GPGVLGRWFPQYYQFVFAPAVVQSLNGVAWWAVMLFVMIAGVELDLKQAWQHRRESGI
                     TAALALGAPLLLGCGAAAVLLKFDGWVGAQAQAWQFVLGIGMACAVTALPILILLMEK
                     LDILRQPLGQRILRYASLDDIAIWGVLALILMDWTRVGKQLAFLLGFVVLGLAYRKLM
                     AKLAEIDRWYVALVWLALCGFGADWAGLHFMVGAFLAGAIMDAHWFNQGKLDQLRHHV
                     LLVMMPVFFLSTGLRTNWAVGGVEVFAVAALLLLVSVAGKLIGVRLAGWLLHWAPGEA
                     SVIGWLLQTKALIMIIFANILLDKHLITSEAFTALLLMAMASTMLTVPLVEPKMRRAR
                     QSLQAKPPHSPFA"
BASE COUNT         3663 a         5352 c         5298 g         3398 t
ORIGIN      
        1 ggacccgaac aaaaatgtca gagactgagg attgtacctc cctaagaatc accttatctt
       61 gactggaaag taacatgttc tccgtgtcgg ccggtcggtt ttctaccagc cgcagggttg
      121 aaaattccat tgccaatcca gtcacggaac ctgtcaatga ctttgccgtg tcgaggcttt
      181 agctgaagct ctccccccca aaaaaacctc gtttcctgat tgaaacccgc attaattttg
      241 gttccgcgtg ctgtctgcgg tttgatctga gtgatcgttt cgagtgcgcg ttttggtatg
      301 tggttgggcg ttgtgatgat ctcaaaagtg gcggcatctg gcgcaccgcc tggcgtgcga
      361 cagcaattgc gcatcatggt ttgcaaagtg tccatcaagg actgaaaact gggcaccggc
      421 gagccatcct cgaaagtgag gctgaggacc ttcttcatcg ccgcatccga gtgtggccgg
      481 tgccaccgag ttacgctcaa gcgtggcggc ttgatccgca tcgctcatcg gcatacatga
      541 gttcacgcca aacctgacgc atgtgccact ccacatagta ggccagcatg cgcaaaaaaa
      601 tgtatgagcg catccgcgcc cttgctcatc tcatcaacgg ccatttgcga gatcatgccc
      661 cgattcccca ccaacaccca agcgcctgat gccaaactcc tcacgcaatc gtgtttcctc
      721 tggcatgaac gtggtgccgt cggaggtgtt acctgcgtgc accgggaccg ccaccgggca
      781 accccggtca tcggtgagca acccgtagtt gacctgcgag atgccttgct tgcggtcacg
      841 attgacgccc aacttgacca agggggcagt gcgagccctc aaagcaactc gacgagaggt
      901 cgtacagcac caaagagtct gctttgaggt gttgttcctt ggagccgcaa ccggcatatt
      961 gacggctcag attggagaaa tcgagtgcgt cgatgacttc cacaacgaag cgcgccagat
     1021 gatcctgggt gagccagtca tctatccatg ggggcagcag gtagtcggta gtcaggcttg
     1081 gggttggtga aaaaaaacct cgacatatcg acaacaatct ctcctaagac acgtccagta
     1141 tagcattaac actacagtaa aaatagcaga acggggggtt tgcgtcagat cggaatttcc
     1201 aacaggtgcc aagtgatata ccaaccgccg ctgcaagttt gagtcgatgg gtcatagtca
     1261 atgcagctct gcaaagactg ggtggccgac atcgactggg cttggtgagt taaacatcaa
     1321 acccgcaccc ggcacaccag catgacgttg gcaaaaggct tgccctcggt catggtgttg
     1381 ctctgcacat caaatccgat atcgtgcagc agttgttgcc agtcccccag gctgcggcaa
     1441 tacaagcgtg acaagcggtg cccgcgcaga aaagtcacca catggtccac ccagtttgac
     1501 acatgaaatg gcaggccagc gctggcgtcg ccaatgcgcg tcaaaaacag gccgcccggg
     1561 ggaagtgcgg catggatgcg ctgcagcacc cgctgctgtt gcccatggtc aaaataatgc
     1621 agcgcatcca ggatggtgat gacatcgggt cggccaaagt caacccggtt catgtcgccc
     1681 tgctcaatcc gcaccagcgg gtgcttggca gcaaaggcca gcgcggcgcg atccacatcg
     1741 ttttgcatca gctcaacgcc acgcaagctc aagggctgcg gtgcagcggg ccagtcggcc
     1801 ggccattggc cttgttcgta taggcggcgt gccgccagca accaggcaaa caagctgcct
     1861 tggccacaac ccaggtccaa aaaatgcccc tgtggcggga agatacctcg cttgagcagc
     1921 tcgcgaaaaa tgcagtcgcc gccaagcttg cctcgggcgt agtggtaggc gaagcggccg
     1981 ctggccagaa aaggctggct cgccaggtcc agcaaggcgg gcatgaagtt ggccgtcatg
     2041 tcaatgcatc tttcagggtc acgggttgta gtccttgcag ggcaaatgcc tgcagcaaca
     2101 ggggcaaggc ggtcagaatc acgggctgcc catcgggtgc gcgcgcgccg tggccgtcgt
     2161 gcattagcag gatgtcgccg gccgcaagac catgggatag ccgctgcagc actcgctgcg
     2221 cccggccctc gcgggtgtca taggcacggc gggtccaact gaccagacgc agttcaagtt
     2281 gcgtcagcac gggttgcaga aacggattgc gcagcccggc ggtcggtcga aaaaacagtg
     2341 gagcctggcc ggtgatgtcg ctcagcgtgg cttgtgccgc agaaatgtcg gcgtgcatac
     2401 gcgccgggcc aaacagcgaa aatgcgttgg aatgcgcgtc gccatgattt tctacccggt
     2461 gaccacgcgc cacgatttcg cggcacagcg ctgggtgttg tcgggcacgc cagccgatgc
     2521 aaaaaaaatt cgccttggca gcgccggtat cgagcagatc gagcacacgc ggtgtgactt
     2581 gtgggtcagg cccgtcgtcg agggtcagcg ccacctgatg gtgggtcagt gccgtagtcg
     2641 gcaggcgagt gagattgggg ccgagcaagg tggagcgcgg tagcaagccc gccagcgtca
     2701 acgctgcgtg attgcccagt actgcagcaa gagtccaagg ccaggccgtt ggcacggcaa
     2761 gcgtgctcaa tgcagcggcg gcatgcaccg cggctgtcgc tttcaggatc ggggtgctgg
     2821 tccagcgccg cgtcatggca gtcgggcaaa gaaacccagg tcaagaaaat gtccccacaa
     2881 ctgccgccag accgcccagt catggccgcc cggcagcgtg cagtggctgg ccgttggcaa
     2941 gcgttctgcc agccaggcca tgccgtcggc aaatcggtcc tgcacaccat aacccataaa
     3001 gaccggcccc tgaatgtcgg ggcgcttgag ccactgccaa agccggaact ccgggtcctg
     3061 caattgctcg gcgctggctt gccaggcatc aatgccaccg ccgcgccgaa tggcattggt
     3121 ggtgagccga ctgcctgcat aaggcgcaag caagcacaag ccatcgactt gcgcagtttg
     3181 ctcagccaag caggataagg ccaactggcc accgcgcgag atgccgccca gccacacgtg
     3241 ctggtagagc gcacgcgctg gcgccagaat gtcggcctcc agggtgtgca gcgccgcgcg
     3301 gttgcaaccc tccagtgcgt gcaaatctgg cacgatcagg tccagcgcca gatcgcgctg
     3361 tgccaccgct tcaaacagtc cggcctgcag cgtgtgctgc ggtgtcatga gcgcgccggg
     3421 cagcatcacc agcagcgtgt tggcgccaca gcggtagaag gcacttgaaa ccaggacttc
     3481 agcctgtgga aacagacaaa aatagcctcc agcccttgat gccattgcgc agtcagctat
     3541 tacttttggg ggcatcttcc ccccgccgga tacaccagca ccgccgacag cagcaaggcc
     3601 agcaccgcgc ccggcccgac tgtcacacca atggcttgca gcaccggcac gcttgacagg
     3661 gccagggcgc caaagccgat ggccgtggtc agcgtcgcca ccgcgatcga cagcagggtg
     3721 gccgcatcca gcggctcacc gcctgcagcg cgatcaaaaa acagcgcgta attggagccc
     3781 acagccacaa tcagcagcat gcccaccaag tgcagcaggt gcaggcgcac gcctgccagg
     3841 tgcaacagtg caatcacccc cagcaccgtc aacaccagcg ccagcaggac gcgcaacagg
     3901 cgcgctctgg agcgtagcgt cagcgccagc aacaccacaa tcgccagaaa accggccagc
     3961 gacagacgca ccgcctggtc catgtagccg ctgtacagcg cgtcaaactc ggctttcagg
     4021 tcaacaaaca gcgcaccgct acccgctagc gcagtgcgta gcttttccgg ggggatatcg
     4081 gcatcgggcg catttgcggc cgggcgcaac ggcatcatca ccgtccagcc gcccgagtgc
     4141 accggcatca gcatcgagtc caccaccagc gccagcgcgc tgccgttcag gtcggcgcgc
     4201 cgcatcacac cggcgctgcg cgcggcctgc acatcggcga caaagggcgc cagcttgtca
     4261 gcgcccagtg gtgaatcgat cagcgccgcg cgcagccggg tctgcaacac gtctgccacg
     4321 ggcaggctga ccaaacgccg ggtttgcagc aaggtgctgg gtagaaagcg tgccgggctg
     4381 tcgtagccgc cgattacacc ttgctccacc aaccgatcaa gctgcgcgcc ggcccgctcg
     4441 gccgcctgca aggcgctttc ttggtctgcc gcaccgatgg tcaccagata gcgtgcatcg
     4501 ggtgccgcca gatcggcgcg catgcgtgag tcggcggcgg cctcagcctg gctcacggtg
     4561 gacagcgcag acaggttggc atcccacaag ctggcgcgtt gctgccacaa atagccgccg
     4621 gccagcagcg ccagtagccc caccagggca cgcaggcggt gcgcatgcac cagcccagtc
     4681 agcacgcgat ggacccacgg gcgcggcgcg ggcacgcaaa attgcgcgcc ggccagggtg
     4741 ggcagcacaa agcgggtcac cagcgcagcg gtcaccaccc cggagagggc gtacaaaccc
     4801 aattgcgaca aacccggaaa gcccgagaaa agcagtgcac caaagcccag cgccgaggtc
     4861 agcacaccca agcgcactgt tggccagaac acccgacgcc agttgtccag ccccatgcgc
     4921 ccggactgca caaagaaata gatggcatag tcaaccgcct cgccgatcag cgctgagcca
     4981 aaccccacgg tgatgccaaa caccgtgcca tgcaccagac tcaccaccgc caccccggcc
     5041 agggcaccgc tgagcatcgg cagcaagccc agtgccagca accgtggcga gcggtacacc
     5101 cacagcagca cacccacaat tcccgccatg ctgacgaaaa acaggcgcga cacctcggcc
     5161 tggatggttt cacgcgcctg caccgcaaac ttgcccgggc ccgaaatctg cagagtcatg
     5221 ccgctgcggt gttgcgccga cagcacctgc gcaaacacct gctgcaagtc ggcaatcacc
     5281 gccgcctgac catccgtgtc tgagccctgg gcgcgagttt gcgccagcag catggcccgc
     5341 tcgccgttgc gcgaagccca gacaccggcg cgggtttccg gttgggcacc ggggttgaat
     5401 tgggcaatca cctccagcaa ctcaccggtc gggtcgcgcg ccaggtaggg cttgaacatc
     5461 agcccggccg gtgacgacag caagtcgatg gtgttggcga ctgccgcgcg cagtccggcc
     5521 tcggtaaagc gatctggcga taccgacgga ctgagctggt agcggtagcg cagcaatacg
     5581 tcgcgctcgc cttcgagccc accagcctcg ccattctgca ccgacacata cgccgggttg
     5641 ttggccaggc gctggcgcag ttcgcgcgac acggaggcac gctgcgcggc atcggcaccc
     5701 tcgatggaca gcatcaacag ccgcgagacc gtgccatctt gtaactgccc gaccagcgcg
     5761 cgctgctcgg cacttggctt ggagggcaaa aaaaacgaca tgtcggccga aaaccggctg
     5821 ttccagacca ccacgctacc ggccagcatg cacagtagcc aaagcagaaa tatccgcagc
     5881 ttcaacgact tcattggttg tcgattggct cgatggtgat ttcggagcgg tcaccatcga
     5941 cttgcaggta ctcgatgtag cgcacctggc tcttgctgcc gttgatggtg atgcgttgca
     6001 gcagtgccga gatggcgggc tcaatgggta ccagggtcag cacccattgc ttactttcgc
     6061 cctgcagatt gagcgtgtag ttccgctcca gagtgcgccg gttgcccgac agcgtgctgc
     6121 ggatgctgtc aacaaaggcg atcgcctcgg gccggctgct taagttgatc gacagcttgc
     6181 gcttgtcgcg ctccaggctg agctgatcct tgtcgagcac catcgtctcg gccttcggca
     6241 gcaaggtgcg cttttcgagc cggtcgggtg ggttgtagag catttcaccg ctggactgca
     6301 ccggcttatc gagcagggcg atgtggcgct tctccacaaa gcgcgcccgc ccgcccttgt
     6361 gctgggtcag ctcggccatc agttgctcaa tgtcgaaagc ggcctgggca ctggtcgcaa
     6421 accccagcaa cagcaggatc aaaccaaagc gtcgaatcat tttttttgcc aaaaatcgaa
     6481 aaaattaaac cagttgtagg gtgccagttg gcacaaacgg gacaggctgt cagcataacg
     6541 ctgtacggca gcctgaattg caccgtcgcg ctggccgcgc tccaccgcag aaaaatcggc
     6601 cagcagctca aaatgcaact ggtaccggtt gcctcccagg tacagcccgg ccatcaggaa
     6661 aaccggtcgt ttcaacatga ctgccatgcg ccacgggccg actggaaaat tggcgctatg
     6721 ccccagaaaa tcggctgcca gcgtggcatc gcctcccaac gagcggtcag ccaacatgcc
     6781 caccagataa ccggcatcga gcttgtcgcg cacggtgagc atcgactcca gccgccccag
     6841 ggcaatgatg tcctgcatgg cgcgcgggtt gatggctgcc agcgtggcat tgagcttgcg
     6901 cgcgttctgc tcatacatca gcatcgccac ctgcagacca tgctggccgc ggcccattgc
     6961 gcgaaggacc tcaaagctgc ccaggtgggc cccaaccagc agggcaccgg cctgcttttg
     7021 cagggctgcc agcaatgcgg gtgcgcctaa cacttggatg tcaaattcat caaagcggtc
     7081 gttgagcaga tagatccggt cgtggatggt gctggcaaag ctgaacacat gtcgatagcc
     7141 atcgcgccaa ccggcgtgcc gacccagggc atgcgtcagg taggcgcgac tggcgcgacg
     7201 ggccatcggt gcaaacagga cgaagtaggt ggcaatgccg cgcagcacca ggcgaccaag
     7261 gcgtcgacca aaagtcagcg agatccagac catcagccgt agcaccagcg cctggctgcg
     7321 ttcctgctgc tgcatccagt cgggccgcgt gtccggattc atcggtgcgg tggcagcaaa
     7381 ctgcccgaag ccatcgtgcg cccggcactg cgcaccgtga atgtcaacac gccgccactg
     7441 ccgcccgaga aggcaaacac cagttcgtcc ccggggccac acgggctcaa aaacttggcc
     7501 tgcctgactt gccaacccga agcgaactgg tctaggtgct gctgcgcgaa cagcagggcc
     7561 agatccagca agaccacccc gggaacgatc ggttgaccag gaaaatgtcc ggcaaatgcc
     7621 gggtgatgca cgggtacatg ccaggtgaac tcggcgtcag ccatgggtct gtttttggta
     7681 caacgcctgc agggcactgc gtggcagttt gccagtgctg atgcgtggca gggactcaac
     7741 aaaaaccagt ggtcgcggca aaaaaatcgg gtcgatgcgc ccacgcagcg ccgccatcag
     7801 ctcagcgcgt tgcatgctgg gcgcaaccac aaaagcgcac aggcgggtat tgcgttcggc
     7861 agcattggaa tcgggcagat aaaacacccc gtcttgcaca cccgggatgg cgcacaactg
     7921 gtggttgagg taggccagcg aggttctttt gcccgcaatg ttgaccaggt cggcgtgtcg
     7981 gccatgtagc aaaaattggc cgtcgctctg caactcgatg atgtctgaca ggggcactcg
     8041 cccctgaacg tgccccccga gcgcataggt agtgtcgtgc gtctgggtta attgcacccc
     8101 aggtagcaga cgccaaagtg gtccatcggt ggtgcgtcgg caggccaatt gcgatgactc
     8161 ggtggagccg tagatctcgt gcaccggtgc gccgctttgg cgctctacct gggcggcaag
     8221 ctcggcactc agcggcgcgg tggctgacag gcacaagtca agcgctggcc agtccagacc
     8281 cgagcccagc aagaccgcca ggtggaaggg ggtggtaacc agcatgcgcg ggcgcggtac
     8341 ggctttcaat gcgtcaacca catcctgcgg gtaaaacggc ttgccgctcc agaagctgca
     8401 gccgccgtgc agtgccagca aaaacgtgga ctcaaagccg tagctgtgct gggccggaac
     8461 ggtaccgacc agcgcgggcg ggtgactgcc aagcgccagt gcagccgcct cggcctggcc
     8521 gttctgcacc agcttgcccc aggtcttgcc atgcggctgg ggcaagccgg ttgagcccga
     8581 tgtgaacaaa attgccaccg tgcggctggc ggcaatcatt ggcatgtcgg tcaccgctgg
     8641 gcgcagcagc gtagccagcc gagggaaggc cagccgcggt aggccgggca actcaaaagc
     8701 cccatcagtg acgcaaaaaa caccgggata gtcttgtgct agccgcaaca gcgtctcgcc
     8761 tgattgcgaa gcaggctgca ggctggtctt gtcggctagc aggcaggccg caaacagcac
     8821 ggcaaagtgg tagcgatctt cacacaggtt aagcacccag ttgcctttgg gcagcagctt
     8881 ggcaacggcc tgcgcatcgg ccagaaatgc acgcaccgtc actggccctg cagggcacca
     8941 ggccagagtg tcgtcaagtt ggtggccgtt gacgataggc agtgcgctca tgcgccggac
     9001 tccggtgact ggcgcgctgc gccgcccatt gcgccgcgct ggcggtaggc gcgaaccaca
     9061 tcggagagac ccgggcgctc atcgggaggc aagacaaacc gtcggaccaa atgctcggcc
     9121 agaaacatcc cggcaatcag tggcccggtc aacaaattgg cgtgtactga ccagacggtc
     9181 ggcgctgccc agagaaacaa gccggtagaa accagggcat tgccaaggaa gaacagcgtc
     9241 caggccaaag tgacttgccg ggtgtatcga accttgcgct cagacagggc gtggtcataa
     9301 atgctgcggg ccatgcgggt gatcaggggc tcgccagacc catgcagggt acgcccaaac
     9361 agcaccgcca gtgccaggtg ggtacccagg tgctgcaggt aatacagcaa cgcgatgctt
     9421 tgcctaagct gcggccacaa ggcaagcact gcgctcagcg ccagcgcaaa gcccgtcagg
     9481 cgcacccagc gcggcttgaa ttgccacaac agcgcggtcc atgccagcag taacggtgtg
     9541 gtggcgatgg ctgcgttcaa atctgcgctg ccaatgtcgg cactccccag atgggccgcc
     9601 accgcccagg ccagcagcaa cacgcctgcc gccgcgccgc gccaggcctg cgaccagctc
     9661 aggctcattt ggtgcgctgg ctggcaatgt agtgggtcag cgcgcgcagt gatgcgtaga
     9721 tctgcgcatt gtcctcattg tctgagcgca gttggaagcc atagtgtttg gagatcacca
     9781 gcgatatttc cagcatgtcg atggaatcaa gccccaggcc ctcaccataa agcggcccgt
     9841 cgggcgaaat gtcgctgact agaacctcca ggttcagggc ttcgatgatc agcgcggcga
     9901 cctcggtcat gagggcatcg tgggaaacag agggaacatc agacatttga gattgttttc
     9961 atcgttgtac agtgctacag gctgtgccca ccatgtcagg atcgcaccgc agaaaagtgc
    10021 ccggcgaccg atctgcaccc aaaaaagttc cgcattttag tgcgcgtttt acaaaagtat
    10081 acgctggcac aatcgcccaa tcgtcatttc aagcgccgtt gtcacggtgt tttcttcatg
    10141 aaatctcaat ggccgcaacg cattaaagac cgcctggttc cctattggcc actcaaggcc
    10201 tttggcacaa cattgttcat ggcgctgttt ttctgggcct attttcaggt gatccatcat
    10261 caatatggtc tgcccatggt catgcccagg atttggctgg atgactggat tggattcaca
    10321 ccgtattcgt tcccggtcta tgcttctttg tgggtgtatg tgtcgctgcc gccggcgttg
    10381 atgggcaatc tcagggaatt gatcctgtat ggactgtggg tggcattgat gtgcctgttc
    10441 tgtttggctt tgttttggct gtttccgacc ctgaccccca cggttgacat ggattggcgc
    10501 aactatccgg cgatggtgat gatcaagggt gtcgatgcgg cgggaaatgc ctgcccctcg
    10561 ctgcatgtgg catcggccgt cttctctgcg tgctggctgg ggcgcatcct tggtcagttg
    10621 cacacgccca aaaccgtgac atggctgagt tgggccattt gcctggcgat tgcctggtcc
    10681 accatggcca cgttgcagca tgttgcgctt gacgtgcttt gtggtgtggc ggtgggtctg
    10741 ggttttgcag tgctttccct gcggcagatc aagaataccg cttgatctga tcgagcttga
    10801 agcaaaagcg tgaacgctgg agcatgtcag agccgaaatg tggggtcgat cgaacaggcc
    10861 atctgcgcct caaaatagtc ttgcaagctg cgtgccgtgt gagcgcaaac cccggtcgcg
    10921 ctcagttgtg cacccaggct ggcttggtag acaagcggaa actgcggtgg gcgaaacagt
    10981 ctccagcctt tgcttaggta gggtgagttg gtccgaatca atatggtttg caagggctgg
    11041 ccggttcttt tggcaatcag ggccacgccg ggcttgatgt cgttgactgg ctggcgtgtg
    11101 gttcgagtgc cctcaggaaa caccaacaag tgcggtccgc ggcgtactgt gttgcttgct
    11161 gagcgcagca tctcgcccgg cttgcggttc gagacatagc ccgccagata ggctccagcg
    11221 ccaaaaagta tgttgctgcc aatgctggcc ttcatcaggc aggcggcgcg cctgaccctt
    11281 gagatcacca aaaaaacgtc gatcatgctg gggtgattgg cgaccagcac cagtcctttg
    11341 tggtggttca gtacatcgag ctgattcaaa tcgagctgca tcaggccact gcgttgcgca
    11401 ccggccaaaa atacccgaaa caggtgactg atcagaccct gcaccaacgg ctcccgaaac
    11461 cagcgcggtg tcagcagcaa aggcagcacc gccacattgc cgatcaacag catcatgccc
    11521 aaccacagca gcatcacata gaacaggcca atttgacgag cgcgtgccat cgaatcctca
    11581 ctggtgcgca caaaacgttt gcccctcggc ttcaatttca agcagcaaat cgcttcggca
    11641 aatgtcggcc tggacaaaac cgaactgcgt catgccccga gtcagcaaat acgcctggat
    11701 caaaggaaaa tcagcagcat ggcgaatgta cacacgtcct tgtaaaccgc ccagtgtcca
    11761 caaatgtgca ccggcacgtt ggttggcacc gtgcaacacc gcctcgatat tggtcaccga
    11821 ttcttggact tgggcacgca cattgcccgc gtgcagggtc cggtggccca caatgctcgc
    11881 cgtgccgctg acaaacaaca ggtcggggcc agacttttgc gccagccacg ctgcccggga
    11941 aaaaatgggc ggatggctac catactgtct tggatagtgg taggcgctga tctggcgcgg
    12001 gttttcaatc gcaatgacag gatgcttgct ggccagcgca gccacgataa gtgcagcgcc
    12061 ccgggttccc agcgcgcagg cggccggtgc cccatcaccc acggtgtaac ccatgctgtc
    12121 aaaagcctgc ctgcgcccga aattaaacag ttggtagcgc tcgatgccgc cgtcaatttc
    12181 gttgatgcgc ggcacatagt tccaaaggcg cagcagatgt ggcttgttga gctgccgcaa
    12241 gcggctcagg agtgcttcat aaacaaggcg gctttgttgt tccagcgcgc cgtgctcgga
    12301 tgaaattgtg gcaaaaacaa aatcttcggt gtgagctacc gtgacacccc gctcgtcctg
    12361 aaccattgag gtgcaagtgt ctgacgcacc atcaacaaac cacgcctgtt gtgcatacgg
    12421 ttcaccgccc aggcagggaa tcggaacttg gcaatgcagc cgcagcgcgc tgccgacagc
    12481 cgccgcttca cccgtggcgg tgcacagcga ccccaaaaac aggccatcgg ggatggcaat
    12541 tgctggttcg ccagccgttt gcccatggtg gacaagcaag ttttgatgac caggcttggg
    12601 caaaatcaat gattccttag gcgaattggt tttccacttc caagaggaaa tggtcgccag
    12661 catacaacat ttttcgcctg caatgacccc attgacaggg tctgaagcgc actgcgcaca
    12721 ccggctgttt cattgcaatc aattcaaaat tgaggtcact gctgtccggt caaaaatcga
    12781 acaaaatcaa ttggttggag atggatctgg ttggttttgt agctcgttga ccggcaaggc
    12841 gcatgaaacc cgggttgtct cgaaaaccga gactgacaac atcagcagac aagtgttgag
    12901 cgaggatttc agttgcagtt cctttttgac aatggcgatc aacacccagg tggcgatggc
    12961 gcaccagatt tgcgtcttca ccccagactc gctggtaccc aagaactttt ttgatgtgca
    13021 gattctgctt gatccacttg acgaacaatt cgacctgccg gcggttctgg taaagctggg
    13081 caatcacagg cgcgggcagc gtcgtgttgt tggtcaaaaa catccgtgtt ttgcccgttt
    13141 gatctttcaa ccgaacccga cgcaaatgct tgggatagtc tttggcggcc ttgaatccat
    13201 tgagcgccac acccggccta aaggcgcaag acatcaagca aattgacatc gcccatcttg
    13261 ccatcgctga cttgaataag ctgggaatgc tgtggcacaa gtccagcagc gcatgcaact
    13321 cgatggcggc acgaaacgct gcccaaccaa acaagctcag gcataaatcg atggtgctcg
    13381 aatccagcgc gcaccgccaa gtggcagttg ctggatgcgc ttggcgggcg ctgagcgcac
    13441 gcaacagacc cggcaaaccg actggtcgca gcggacaaac cgactatgat ccctcaagca
    13501 aattgcgcat caacaagctt caaccaacgg atcattcagt gcagaataca accatcaagc
    13561 cgcctccaaa tgccacaccc ttggtctgtg acgttctggt cattggcggt ggcccggccg
    13621 gctcgaccgt ttccagtctg ctggccgagc agggccacaa ggttgtggtg ctagagaaaa
    13681 gccaccatcc gcgttttcat attggcgagt cgctgctgcc agcgaacttg cccttgttcg
    13741 aagccatggg cattgccgac gaggtcaagg ccattggcat gttcaagccg ggggccgagt
    13801 ttgtctcgcc aacccacaac gccaagcaga cctttcattt cgccgatgcc tggaacaagt
    13861 ccatgcccca cgcctaccag gtcaagcgcg ccgagtttga tgagattctg attcgccacg
    13921 cggcgcgcaa gggcgctgaa gttcacgaag gatgcaaagt gacagcggtt gactttttgg
    13981 ccgatggtgg tgcatgcatc acagcacgcc acgacgacga gcgcgtgcag caatggcaag
    14041 ctcgttttgt ggtggacgca tccgggcgcg acaccttttt tgccaaccgc ttcaagatca
    14101 agaaaagcaa ccccaaacac aacagctcgg cggtctatgc ccacttcagc caggcgcagc
    14161 gcaacgaagg tcaggacgaa ggcaacatca ccatcttctg gtttgaccat ggctggttct
    14221 ggttcattcc gctgaccaat ggcactacca gcgtcggcat ggtgacttgg ccataccaca
    14281 tcaagacccg cggccagcgc agcctggagc agtttttgat ggacaacatc gccaccagcc
    14341 caaccttgag cgcccgactg caaaatgcca cccgtgtcag cccggtcgag gccaccggta
    14401 atttttctta tgtatctgag cgaaatcatg gtgccaacta tctgatgctg ggtgatgcct
    14461 atgcctttat tgacccggtt ttttcgtccg gcgtctggct ggccatgaac agtgcggtga
    14521 ttggtgccga ggccatcagc agttgcttga aaaatccagc caagtcggca gtggcactgg
    14581 cgcggtttga ccgcctgatg cgccacgggc cgcgcgaatt ttcgtggttt atctaccgcg
    14641 tcaccaaccc catcatgcgc gactttttca tgcacccgaa gaacgtgatg cgggtcaagg
    14701 aagcgctgct ctcggtgctg gcaggcgaca tatttggcaa gacaccgatc tggcgctcga
    14761 tactggcgtt caagatgctc tattacagtg ccaatattct gcagccccga cgcgccttca
    14821 gggcctggca gcagcgccgc ttcaacatcc gcaaagtgga tgacgcggtt gtgtacaacg
    14881 cctgacttgc caccggcatc gtgcggcctt gagtcaatgt ccgaaccgct ttgcactgcc
    14941 tccaccagcc atttggtgct gatccccagc tacaaccccg gcccgaatgt gctcgatacg
    15001 gtgcgccagg cacgccgatt ctggcatccg gtctgggtgg tggttgatgg cagcaccgat
    15061 ggcaccggtc tgcaactcca ggcactggct caaaccgatg ctggtctcag ggtgattgtg
    15121 ttaccgcaca accagggcaa gggcgccgcc gtgctgcacg gcatcacatt ggcggcgaag
    15181 gcgggtttca ctcatgtcct gaccatggac agcgacggtc agcatccagc cgaggcgatc
    15241 cccgcgttca tggctgcctc acgacatgag cccgctggga tggtgctggg caaaccggtg
    15301 tttggaccgg aggcaccgac gttgcgtgtc aatggccgca aggtgtccaa cggttgggcc
    15361 aatctggaaa ccctgtggat gggcattggt gactcgctct atggatttcg tgtttaccca
    15421 atcgcacccc tgctgcaggt gatgcagggc aaccgcttca tgcgccgctt tgactttgac
    15481 cctgaagccg tggtgcgcct gtgctgggcc ggggttcacc cgattaacat cgatgccccg
    15541 gtgcgctatc tgtcggctca ggagggtggc gtgtcgcact tcaactacct gcgcgacaac
    15601 gtgctgctga cctggatgca cacccgcctg ttgctgggct ttctgttgcg cttgccactg
    15661 ctgatctggc gtcgtgccac caagaactga catgaataaa cccgtcgcgc gcaccagtgc
    15721 gctcaagtcc gaccaccgca tggaccccta cttcaccacc gaagagggtc gccgcagctt
    15781 gacacaggcc atgttcaacc aagctgcacc cggttacgac aacgccgaga gtctcaccgc
    15841 gttgggcagt ggtgtctggt atcggcgtga ggtgttgctg cgcaacggtt tggcagccgg
    15901 catgaccctg cttgacgtgg cggccggcac cggtctggtc acggtgcctg gccatgcgat
    15961 tgtgggcccg caggggcggg tgctggcgct tgacccgtcg cctggcatgc tggctgagct
    16021 gcgcaagaaa attcaggttg agaccatcga agcatttgcc gagtcaattc ccttgcccga
    16081 cgggcagatc gatttcatat cgatgggcta cgcactgcgt catgtcggtg atttgaattt
    16141 ggcgtttgcc gagtactttc gcgtgctgtg cccgggtggg cgcgtctgca ttatggaaat
    16201 cagccgcccg cgcagcgccc tggcgcgtgc cttgctgggc tttcacatca agatggtggt
    16261 gccgctgctg gcgcgtctga cgcgccgcca tgcggatatc caacgcctgt gggaatatta
    16321 cggcgacacc attgaggccg cgcttgagcc cgaactcatc ctggatgcct tgcgccgcgc
    16381 cggctttacc gatgtagcgt gctgcgtcac cctgggcatt ttccgcgagt acaccggacg
    16441 ccgaccttga acaccaccga ggttttcctg attgcgatgc tgatcatctt cagcatccct
    16501 tggctgatct ggcgtctcgg caacaccgac tactttgcac ccttggtggt ggtgcaaatc
    16561 ctcgctggca tcgtgctcgg gcccggtgtg ctgggccgct ggtttccgca gtactaccag
    16621 tttgtttttg cgccagcggt ggtgcagtct cttaacggcg ttgcctggtg ggcggtgatg
    16681 ctgtttgtaa tgattgccgg cgttgaactg gatctgaaac aggcctggca acaccggcgc
    16741 gagagcggga tcaccgccgc tctggcattg ggtgcgccgc tgctgctggg ttgtggtgcc
    16801 gcagccgtgc tgctgaagtt tgacggttgg gtgggtgcgc aggcgcaggc ttggcaattt
    16861 gtgctgggaa tcggcatggc ctgcgccgtg accgccttgc cgattttgat cctgctgatg
    16921 gaaaagcttg acatcctgcg ccagccgctg ggccagcgta ttttgcgcta cgccagcctc
    16981 gacgacattg ccatctgggg cgtgctggcg ctgattctca tggactggac ccgcgtcggc
    17041 aagcagttgg catttctgct gggttttgtg gtgctgggtc tggcctatcg caagctgatg
    17101 gccaagctgg cggaaattga ccgctggtat gtggcgctgg tctggctggc cctgtgcggc
    17161 tttggtgctg actgggccgg cctgcatttc atggtggggg catttctggc cggagccatc
    17221 atggatgccc attggttcaa tcagggcaag ctcgaccagt tgcgccacca tgtgctgttg
    17281 gtgatgatgc cagtattttt tctctcaacc gggctacgca ccaactgggc agtcggcggc
    17341 gtggaggtgt ttgccgtcgc cgcgctgctg ctgctggtgt cggtggcggg caagctgatc
    17401 ggggtgcgcc tggctggctg gctactgcac tgggcaccgg gtgaagcctc ggtgattggc
    17461 tggctgttgc aaaccaaggc gttgatcatg atcatctttg ccaacatcct gctggacaaa
    17521 catctcatca ccagcgaagc cttcaccgcc ttgctgctga tggccatggc cagcaccatg
    17581 ctgaccgtgc ccttggtcga gccgaagatg cgccgggcgc gccagtcgct gcaggcaaag
    17641 ccgccgcact cgccattcgc ctgaatgcta caataacaat agctgtttgc gcttgtcaaa
    17701 taagggctgg c
//
LOCUS       DAOSLL010000012        17571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_3437_length_17571_cov_4.568908, whole genome shotgun sequence.
ACCESSION   DAOSLL010000012 DAOSLL010000000
VERSION     DAOSLL010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 17571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17571
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3437_length_17571_cov_4.568908"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PLB25_01015"
     CDS             complement(<1..618)
                     /locus_tag="PLB25_01015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOE40206.1"
                     /translation="MVNPQAIEVRRELVAIYEVSKTLASSLDVAKTFREALNYLIHAF
                     DWRRAFVLLPEPGASLRGLCAVGLTREEQQRLHYQSGEGIVGRVFANGIQLMVPDVRS
                     EPLFLNRTGGIDPFSDVPVAMLLTPISTEGRIVGLLVIDCLNPNEQRMFSNDLQLLKM
                     VATLMGQALLLHRHVSDVHVNLQDEVRRMRKVLKPARQIDHVVGVS"
     gene            885..2129
                     /locus_tag="PLB25_01020"
     CDS             885..2129
                     /locus_tag="PLB25_01020"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HOE40207.1"
                     /translation="MSDPRAASSLAKATPPVYLDYAATTPVDHRVASKMMPYLTQIYG
                     NPASRSHAYGWAADEAVELAREQVCALINADPREIAWTSGATESNNLAIKGAAHFYRG
                     KGKHLVTVATEHKAVLDSMRELEREGFEVTVLPVLPTGVLDVAVFEAALRPDTLLASV
                     MMVNNETGVIQDVAALGAICRAKKVLFHVDAAQAAGKVEIDLGVLPIDLMSLSAHKIY
                     GPKGVGALYVRRKPRVRIEAQMHGGGHERGMRSGTLPTHQIVGMGEAFWLARESMAAD
                     NARMLALRERLWAGFKKMDAVVLNGDLQQRAAQYLNVSFNYVEGESLLMGIKDVAVSS
                     GSACTSASLEPSYVLRAMGRSDELAHSSVRFSIGRFTTVQEIDFTIAQVTQVVTRLRA
                     MSPLWDMVQAGVDPNSIQWSAH"
     gene            2173..2607
                     /gene="iscU"
                     /locus_tag="PLB25_01025"
     CDS             2173..2607
                     /gene="iscU"
                     /locus_tag="PLB25_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HOE40208.1"
                     /translation="MAYSEKVVDHYENPRNVGAFEAGDDSVGTGMVGAPACGDVMRLQ
                     IRVNAQGVIEDAKFKTYGCGSAIASSSLVTEWVRGRTLDEALAIKNADISEELALPPV
                     KIHCSILAEDAIKAAVADFRSRHPDGAATLVSEQTACASSTR"
     gene            2650..3039
                     /gene="erpA"
                     /locus_tag="PLB25_01030"
     CDS             2650..3039
                     /gene="erpA"
                     /locus_tag="PLB25_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HOE40209.1"
                     /translation="MQAALSVAGPAMPDGVPEVMPGMLEFTAQAAAKVASLITEEGNP
                     ALKLRLYVTGGGCSGFSYGFAFDDKIAEDDTLTVTEGVALVVDAISLQYVLGARVDFE
                     EGLEGSRFVIHNPNAQTTCGCGSSFSM"
     gene            3051..3374
                     /gene="iscA"
                     /locus_tag="PLB25_01035"
     CDS             3051..3374
                     /gene="iscA"
                     /locus_tag="PLB25_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017904917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HOE40210.1"
                     /translation="MSVSLTPAAARHVEKSLTRRGSGIGLRLAVKTSGCNGFAYALEF
                     VDAVNAEDECFETHGVKVIVDPRSLGMLAGTELDFVREGLNEGFKFTNPNATANCGCG
                     ESFAV"
     gene            3392..5266
                     /gene="hscA"
                     /locus_tag="PLB25_01040"
     CDS             3392..5266
                     /gene="hscA"
                     /locus_tag="PLB25_01040"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HOE40211.1"
                     /translation="MALLQIAEPGQAAAPHQHRLAAGIDLGTTNSLIATVQSAVARAL
                     PDPDGALLLPSVVRYLPGNGIVVGREAQQSQAEDPQNTIVSVKRFMGRSLADVKQAAG
                     LPYRFVDAPGMVGISTVSGERSPVQVSAEILTALRERAEVSLGGPLAGVVITVPAYFD
                     DAQRQATKDAARLAGLTVLRLLNEPTAAAIAYGLDKAAQGTFAIYDLGGGTFDISILR
                     LTKGVFEVLATGGDSALGGDDFDYCVAEWFCTQHGLAGLSAMPPGAQRSLLAAARKAK
                     EALSGHESAALSLAGPDGATRQAILSRAKFAELGAALIARTLSATRRALRDAQLGIED
                     ITGVVLVGGSTRMPIARDAVRGFFERDPLADIDPDQVVAIGAALQANVLAGNSSADEW
                     LLLDVCPLSLGIETMGGLVEKIIPRNSTLPIARAQEFTTFRDGQTAMSLHVVQGERET
                     VANCRSLARFELRGIAPAVAGSVHIRVSFQIDADGLLSVTAREQASGIEAHIEVKPTY
                     GLADTHISAMLADAIGASKADLLLRSLREAQVDARRMMDATETALNQDAALLTEPEIW
                     AIHQAMFKVGDALETDAPLAELRVATAALAGVTNDFAARRMNASICKALTGQSMDSLA
                     "
     gene            complement(5403..5576)
                     /locus_tag="PLB25_01045"
     CDS             complement(5403..5576)
                     /locus_tag="PLB25_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40212.1"
                     /translation="MASCQMAQVGASPVVNDGACCHVHVPITAPVGARKRAWARKGVW
                     TVMICLAPVAISG"
     gene            5567..6259
                     /locus_tag="PLB25_01050"
     CDS             5567..6259
                     /locus_tag="PLB25_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HOE40213.1"
                     /translation="MMPSRASVMLWTADLQAWQEAPAAWLADLDDAERAQCQRFRRPG
                     DRLAYVAAHALLRRALGAILGLPAASLRFARDALGKPFLQPPGSIDFSLSHTTGMVAV
                     ALSSAGPVGVDVEALDRTRVPMQDWGAFGLSAAQERHLATLAGPQQNQAFLALWTARE
                     AVSKADGRALSLPLQCIQVDLCGKSATVLAPAAEPARRWRLWCDQPSGRHVLALAWQQ
                     NGSDMQQMGTLL"
     gene            complement(6465..6713)
                     /locus_tag="PLB25_01055"
     CDS             complement(6465..6713)
                     /locus_tag="PLB25_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40214.1"
                     /translation="MVVVGAGRIHLDGIAQTEVNDPLAKHAIGGRASADIAHADKKNG
                     ITHGKNLMWVSGATAKQVSPPDDTCKLDDCSIALRFVA"
     gene            6600..7076
                     /locus_tag="PLB25_01060"
     CDS             6600..7076
                     /locus_tag="PLB25_01060"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HOE40215.1"
                     /translation="MGNICRSPTADGVFRQRVIDFGLSDAVKVDSAGTHNYHPNSPPD
                     KRSQAHAAKRGYDLSKLRARKILHADYTAFDLILAMDRDNLAMLKAACPMAHQHKLRR
                     LTEFCLRLNSPEVPDPYYGDARGFEVVLDLIEDACDGLIRHVVRQVNQKTEISSAG"
     gene            complement(7197..7619)
                     /locus_tag="PLB25_01065"
                     /pseudo
     CDS             complement(7197..7619)
                     /locus_tag="PLB25_01065"
                     /inference="COORDINATES: protein motif:HMM:NF016430.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
     gene            7756..8358
                     /locus_tag="PLB25_01070"
     CDS             7756..8358
                     /locus_tag="PLB25_01070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HOE40216.1"
                     /translation="MMPAPDNPSPDMVSTMLTLITTRQTILPKRLSEPGPSAWQVNDM
                     FRAAAAAPDHHMIMPWRFVLVPPDRRAALADVFAQALLERDAAATPEQVEQACEKAFR
                     SPFLALAIARLGLCEPDVDPLERMVSVGAAIQNFLLVAHSMGFGASLTSGQAMRSAAL
                     RQLFQLLEGEQAVCCLNVGTANKRKPARLLPDVSAFVSSL"
     gene            8527..9951
                     /locus_tag="PLB25_01075"
     CDS             8527..9951
                     /locus_tag="PLB25_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HOE40217.1"
                     /translation="MQQIAPKPASADVAPTGRHPGLPPDDAQRVALHNEMHARPSARI
                     RLPALIIDIAVLHDGVTRELEHAHLRSLPGQANLLPQQLQDNFLRLRGEGFSVKWERH
                     TEFTRYTIVGPLPSQALLHVAEPDLMRNLAVPVAWLQQIPGRTVSAVQLVMLNAPLDD
                     AKALMSQAMTWLGAHTVVASLMGSNAFQAVEPGGQPAGLQGYRHPLGHSWALTQFQLQ
                     DDGFERMLVIAPEGTSQTRAGRIAGRLLELETYRLMALRGLPVAKALGPMLSQAESAL
                     ASITAQLENKSASDQQLLDTLVSLAAGVERAMAENVYRFAATQAYYNIVVQRITELRE
                     KAIPGTQMIGEFIQHRLSPAMATVVATNSRLTSLSERVARTSDLLRTRVDIATEVQNQ
                     QLLEKLSRGQELQLRLQSTVEGLSIAAISYYVISLLLYGGKALKAAGVPINPEMAAGA
                     LVPVVLWLVWRTTQRIHAGLHVGH"
     gene            complement(10378..11607)
                     /locus_tag="PLB25_01080"
     CDS             complement(10378..11607)
                     /locus_tag="PLB25_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40218.1"
                     /translation="MFNSSHSIRLILASTLTAVLMAACGGGGDPAPPPAAGLQLDPGD
                     GRVTISWVAAPGVEYWLGYQAAATVSLAPGTPHTWVINVTSPYVLPGLTNGTTYAFSM
                     NGRTDGGPGGAATPSVATVPRPGGESWTIGSTLGTSAIRSVAYGTASDSTVDYVAVGD
                     AGAIYKGTDGINWSAVSTGPKVDFRAATYALSKFIAAGAGGQIYYSTDIATWTAGVSG
                     TSNNLNALASNGTLVVAVGDNGTIRYSSDGATWTAAASVPSSSHLNGITYAASGLWLA
                     VGANGTVLTSSDGMTWTAQISGTSQALTGVATQFTSVYTYVAVGAAGTVLRSNDGITW
                     SIAALSPAVNLTGIVVPSTNSQFLAIGEAGAVRTSPDGITWTARSTTTTANLWGLITA
                     QVQYVAVGDAGITISSH"
     gene            11967..12428
                     /locus_tag="PLB25_01085"
     CDS             11967..12428
                     /locus_tag="PLB25_01085"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOE40219.1"
                     /translation="MKAFQDYYPENLSHCYGCGAHNEHGHRIKTFWDGDETVTHFTPE
                     PFHTAVPGFTYGGLLASLIDCHGTGTAAAAMYRQAGRDMDTLPGFRFVTGSLHVDYLK
                     PTPISGPLEIRGRVKEIKGRKVVVEATVYAAGVATARGEVVAIQMPENFGA"
     gene            complement(12463..13443)
                     /locus_tag="PLB25_01090"
     CDS             complement(12463..13443)
                     /locus_tag="PLB25_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HOE40220.1"
                     /translation="MLAAAISALSLAPIYSAAQELIQPIKPPMQINLGMVELGKKLYF
                     DPRLSKSGFISCNSCHNLSMGGTDNIPTSIGDKWQQGPINAPTVLNASLNVAQFWDGR
                     AADLKVQAGGPIANPGEMGFSHTLAIGVLESIPAYVREFRQVFGKDKVDIDQVTLAIA
                     EFEKTLVTPNSRFDQWLLGRADALSADELAGYKLFKESGCVACHNGPAVGGASFQKMG
                     VVAPYKSTGGAEGRSAVTGKEVDRFNFKVPTLRNVEMTYPYFHDGAVNTLPEAVDIMG
                     RLQLGKTFTKDENAKVVAFLKSLTGEQPSFMLPILPPSSDKTPRPTPFGK"
     gene            13645..14550
                     /locus_tag="PLB25_01095"
     CDS             13645..14550
                     /locus_tag="PLB25_01095"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOE40221.1"
                     /translation="MKLSALRYLVAFSDQLSFSRAAEQCQVSHPSLSVALQRLEEELG
                     VILIDRSKHHMSLTEVGERMVTQARKSLEEVGCIETVAQSGKDPLDDTFRLGIIHTIA
                     PYLLPDLIPALHRVAPGLTLYIEESMTALLADYLKYGTVDGAIVALPFDIPGIATQAL
                     YDEVFEVAVPKGHPWAARKHIGASEIGSEDVLVLKAGNCFREQVLDACPEISHTNTSR
                     HQGHSIETIRAMVASGYGISVLPKSALSELYQSDLVVSIPFEDPPPLRRVALAWRRSS
                     VHEKVVQVLLQAVHAMNKSAYKIVH"
     gene            complement(14747..15067)
                     /locus_tag="PLB25_01100"
     CDS             complement(14747..15067)
                     /locus_tag="PLB25_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40222.1"
                     /translation="MHTRALASGPAPGWQAIDGDGGHSVVVIEHDLDVIAEADWALDL
                     GPVSGNAGGRVVAAATPEGVVALGTHTAVAPGYRCATGWRYWIRRCANSYYQHSCQRN
                     MDKG"
     gene            15058..15246
                     /locus_tag="PLB25_01105"
     CDS             15058..15246
                     /locus_tag="PLB25_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOE40223.1"
                     /translation="MYAPNYSNNSQNSHASVNNAIAASTGDNDIFASIEKLLALQSRG
                     ILSEQEYASKKAELLSRL"
     gene            15487..16467
                     /locus_tag="PLB25_01110"
     CDS             15487..16467
                     /locus_tag="PLB25_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40224.1"
                     /translation="MKRRTALWMLACPAASRAAHRRILVVESYHAEYPWDQDYREVLQ
                     ALLGERYSLDFFEMDTKRLPTDMHAGMAQKAMTWILNVRPELVILGDDAALYYLGEPL
                     DKLNIPVVYLGINGNPRNYGQLKFKNITGVLERPLIKRNIVFMQSIMPSLRKVLVLFD
                     NDLTSQLTYDELFDGRPSIMIDGIQADLKLCKTLDDWKHSILSAPAKGYQATIAGLYH
                     TLKLPNGKVADSDKVISWASANTPLPLFALWGFAVGEHKAIGGLVLSSREQGRMAASI
                     ALAILEQGVKPSERQPITARQGDFVFSKAELARFNLVLPKDIAAQAQLLN"
     gene            complement(16579..16758)
                     /locus_tag="PLB25_01115"
     CDS             complement(16579..16758)
                     /locus_tag="PLB25_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40225.1"
                     /translation="MAIKGRKLGISFWEYLQNRVFLTTAIPPLPMLIRNAVVSGALPW
                     LFERFRINHKKVVTT"
     gene            complement(16918..17181)
                     /locus_tag="PLB25_01120"
     CDS             complement(16918..17181)
                     /locus_tag="PLB25_01120"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOE40226.1"
                     /translation="MSTPTPQTQIPATGRRHERYSDEFKRQVVAACHEPDVSKAASAL
                     AHSLNANMLRRWVVQSPQVSHGQLVAPTKSPLMATQFPPPVAT"
BASE COUNT         3531 a         5258 c         5249 g         3533 t
ORIGIN      
        1 ggacaccccg accacatggt caatctgccg cgctggcttg agcaccttgc gcatgcgccg
       61 cacctcgtct tgcaaattaa catgcacatc gctgacatgg cggtgcagca gcagggcctg
      121 ccccatcagc gtggcgacca tcttgagcaa ctgcaaatcg ttggagaaca tgcgttgctc
      181 gttcgggttc aggcagtcaa tgaccagcag tccgacaatc cgcccttcgg tgctgatggg
      241 tgtcagcagc atcgccaccg gcacatcgga aaacgggtca attccgccgg tacggttgag
      301 aaacaagggc tcgctgcgga catcgggcac catcaactgg atgccattgg caaacacccg
      361 gccgacaatg ccttcgccag actggtagtg caggcgctgc tgctcttcgc gcgtgaggcc
      421 gacggcacac agcccgcgca gcgacgcgcc cggctctggc agcagcacaa aggcacgccg
      481 ccagtcgaac gcgtgaatca ggtaattcag ggcttcgcgg aaagttttgg cgacatcgag
      541 cgaactggcc agcgtcttgc tgacttcgta aatcgcaacc agctcgcgcc gaacttcaat
      601 tgcttgcgga ttcaccattt gaggctcctg ttagcgcaac acgatgccaa tacatcggtt
      661 atcgggcgaa atcatgcaag aaacgcacca aaaatgccga cgaattggat tgcggacagt
      721 caaaccgcct gtttgcgaca aaccagacaa gccaaccagc gccctgtggc caggcgctgt
      781 gacaaaaaca acagtgaaca cgccaaatgt tcggatttgc ccggcatttg gtgtggcact
      841 gaaattgcaa aacaggtgat gtccaacttt ttcaaagagt cgccatgtca gatccccgcg
      901 ctgccagcag ccttgcaaag gccaccccgc ccgtctatct tgactatgct gccaccacac
      961 cggtggatca ccgggtggcc agcaaaatga tgccgtacct gacacagatc tacggcaacc
     1021 cggccagtcg ctcgcacgcc tatggctggg ccgctgatga ggcggtggaa ctggcacgcg
     1081 agcaggtctg cgcactgatc aacgccgacc cgcgcgagat tgcctggaca tcgggtgcca
     1141 ccgagtcgaa caatctggcc atcaagggtg ccgcgcattt ctaccgtggc aaaggcaaac
     1201 atctggtcac agtggccacc gagcacaagg cggtgctcga cagcatgcgc gaactcgagc
     1261 gcgagggctt tgaggtaacg gtgctgccgg tgctgcccac tggtgtgctt gatgttgcgg
     1321 tgtttgaagc ggcgttgcgc cccgatacgc tgctggcttc ggtgatgatg gtcaacaacg
     1381 aaaccggtgt cattcaggat gtggccgctt tgggtgcaat ttgccgcgcc aaaaaggtgc
     1441 tgtttcatgt cgatgctgca caggctgcgg gcaaggtgga gattgacctg ggggtgttgc
     1501 cgattgacct gatgtccttg tcggcccaca agatttatgg ccccaaaggc gttggcgcac
     1561 tttacgtgcg gcgcaaaccc cgcgtgcgaa tcgaggccca gatgcacggc ggtggccacg
     1621 agcgtggcat gcgctccggc accttgccaa cgcaccagat tgttggcatg ggcgaagcct
     1681 tctggctggc gcgcgaatcc atggctgccg ataacgcgcg catgctggcc ttgcgcgagc
     1741 ggctctgggc cggtttcaaa aaaatggacg cggtggtgct caacggtgac ttgcagcagc
     1801 gggcggcgca atacctgaac gtcagcttca actatgtcga gggcgagtcg ctgctgatgg
     1861 gcatcaagga tgtggcggtg tcgtccggtt cggcctgcac ctcggcctcg cttgaaccca
     1921 gctatgtgct gcgcgccatg ggccgcagtg acgagctggc gcacagctcg gtgcgttttt
     1981 ccatcggccg cttcaccacg gtgcaagaga tcgacttcac gatcgcgcag gtcacccagg
     2041 tggttaccag acttcgcgcc atgagcccgc tgtgggacat ggtgcaagcc ggtgttgacc
     2101 ccaactcaat ccagtggtcg gcccattgag cctggcctgc cctttggccc accctactta
     2161 cggagaaact tcatggctta tagcgaaaag gtggttgatc actacgagaa cccgcgcaat
     2221 gtcggggctt ttgaggcggg cgacgacagc gtcggcaccg gcatggttgg cgcgcccgct
     2281 tgtggcgacg tgatgcgcct gcagattcgc gtcaatgccc agggcgtgat tgaagatgcc
     2341 aaattcaaaa cctatggctg cggctcggcc atcgcgtcaa gctcattggt gaccgaatgg
     2401 gtgcgaggcc gcacgctgga cgaagcgctg gccatcaaga atgcggacat ttccgaagag
     2461 ctggccttgc caccggtcaa gatccattgc tcgatcctgg ccgaagacgc gatcaaggcg
     2521 gcggtggctg actttcgttc gcgccacccc gacggtgccg ccacgctggt gtcagaacag
     2581 actgcctgcg cttcaagcac ccgctgaagc cacccttagc cccatctccc acccaaccaa
     2641 ggaatcaaaa tgcaagcagc tctttcggtg gccggtcctg ccatgcccga tggcgtgcca
     2701 gaggtcatgc ccggcatgct cgaattcact gcccaggcgg cagccaaggt ggcatcgctg
     2761 atcaccgaag aaggcaaccc ggcgctcaag ctgcgcctgt acgtcaccgg tggtggctgc
     2821 tcgggctttt cctacggttt tgcctttgac gacaagattg ccgaagatga cacgttgacc
     2881 gtcaccgaag gcgtggcgct ggtcgtcgat gccatcagtt tgcagtatgt gctgggggcc
     2941 agggttgatt ttgaagaagg cctggagggc tcgcgctttg tcattcacaa ccccaacgcc
     3001 cagaccacct gtggctgcgg cagttcgttt tcaatgtaag aggtgatgcc atgagtgttt
     3061 cgctgacccc ggctgctgcg cgccacgtcg agaagtccct gaccaggcgc ggcagcggca
     3121 ttggcctgcg gctggcggtg aagaccagtg gctgcaacgg ctttgcttat gcgctggaat
     3181 ttgtcgatgc cgtcaacgct gaagacgagt gctttgagac ccacggtgtc aaagtcattg
     3241 ttgacccgcg cagccttggc atgctggcag gcaccgaact cgactttgtc cgtgagggcc
     3301 tcaacgaagg cttcaaattc accaacccga acgccaccgc caattgcggt tgtggcgaaa
     3361 gcttcgcggt ctgaaccagg gaacccgcca catggcactg ctgcaaatcg ccgaacccgg
     3421 ccaggccgcc gcaccgcacc agcaccggct ggcagcgggc attgacctgg gcaccaccaa
     3481 ctcgctgatt gccacggtgc aaagtgccgt ggcaagggca ttgcccgacc cagacggtgc
     3541 cttgctgctg ccatcggtgg tgcgctatct gccgggcaac ggcattgtgg tggggcgcga
     3601 agcgcagcaa agccaggccg aagacccgca aaacaccatt gtgtcggtca agcgtttcat
     3661 gggccgcagc ctcgccgacg tgaagcaggc cgctggcctg ccctaccgtt ttgtcgatgc
     3721 gcccggcatg gttggcatca gcacggtgtc tggcgagcgc tcgccggtgc aggtctcagc
     3781 cgagattctg accgccctgc gcgagcgcgc tgaagtctcg ctcggtggtc cgctggcagg
     3841 ggtggtgatc accgtgccag cctactttga cgatgcccag cgccaggcca ccaaagatgc
     3901 ggcgcgtctg gccgggctga ccgtgttgcg cctgctcaac gagcccaccg ctgcggcaat
     3961 tgcctacggg ctggacaagg ccgcccaggg cacttttgcg atttacgacc tgggcggcgg
     4021 gacttttgac atttccatcc tgcgcctgac caagggcgtg tttgaggtgc tggccactgg
     4081 cggcgactcg gcgctgggtg gtgatgactt tgactattgc gtggccgagt ggttttgtac
     4141 ccagcatggt cttgccggtt tgtcggcgat gccgccaggt gcccagcgca gcctgctggc
     4201 agccgcccgc aaagccaaag aagcactgtc tggccatgaa tcagcggcgc tgtcattggc
     4261 cgggcctgac ggggccacgc gccaggccat cctgtcacgc gccaagtttg ccgaactcgg
     4321 tgccgcgctg atcgcccgca ccttgtcggc cacccggcgg gcgttgcgcg atgcccaatt
     4381 gggcattgaa gacatcaccg gcgtggtgct ggtgggtggc tcgacccgca tgccaatcgc
     4441 ccgcgatgca gtgcgcggat ttttcgagcg cgacccgctg gcagacattg acccggacca
     4501 ggtggttgcc atcggcgcgg cgttgcaggc caatgtgctg gctggcaaca gcagtgccga
     4561 tgaatggctg ctgctcgatg tctgcccgct gtcgctgggg atcgagacca tgggcggcct
     4621 ggttgaaaaa atcatcccgc gcaactcgac tttgccgatt gcccgcgcgc aggaattcac
     4681 caccttcagg gacggccaga ccgccatgtc gctgcatgtg gtgcagggcg agcgcgaaac
     4741 ggtggccaac tgccgatcac tggcccgctt tgagctgcgt ggcattgcgc ccgcagtggc
     4801 tggctcggtt cacatccggg tcagtttcca gatcgatgcc gacggtctgt tgtctgtcac
     4861 ggcgcgtgag caggccagcg gcattgaggc ccacatcgag gtcaagccta cctacggtct
     4921 ggcagacacg cacatttcgg cgatgctggc agatgccatt ggcgcgtcca aggcagacct
     4981 gctgctgcgc tccctgcgcg aggcacaggt ggatgcaagg cgcatgatgg atgccaccga
     5041 gaccgcactc aatcaggatg cagctctgct gacagagcca gagatctggg ccattcacca
     5101 ggccatgttc aaggtgggtg atgcgctgga aaccgatgcg ccacttgccg agctgcgggt
     5161 tgcaaccgcc gcgctggctg gtgtcaccaa tgattttgcg gcccggcgca tgaacgcctc
     5221 gatctgcaag gcgctgactg gtcagagcat ggacagcttg gcctgagccg ggcacccaag
     5281 cggctgcgcg ccgttttttc aattctgccg ggtactttgc cgccgccccg gcgaccggca
     5341 ggatacgatt ggcgcatcga tttcaggctg cgctagcgtg cccaacggcc attgccggtc
     5401 aatcagccag aaattgccac tggagcaagg caaatcatga cagtccagac ccctttgcgc
     5461 gcccatgccc gcttgcgggc accgaccggt gccgtgatcg gcacatgaac gtggcagcac
     5521 gcgccatcgt tgaccaccgg gctggcaccc acctgggcca tttgacatga tgccatcgcg
     5581 cgcgtcggtg atgctatgga cggccgactt gcaagcctgg caagaggccc cggcggcgtg
     5641 gctggcggac ctggatgatg ccgagcgtgc ccaatgccaa cgctttcgca ggccaggcga
     5701 ccggctggcg tatgtggccg cgcacgcctt gttgcgccgc gcactcgggg ccatcctggg
     5761 gttgccagct gcatccctgc ggtttgcgcg tgacgcactg ggcaagcctt ttttgcagcc
     5821 gcccggcagc atcgatttca gcttgtcgca caccaccggg atggttgctg tggcgctaag
     5881 cagcgccggg ccggttgggg tggatgttga agcgcttgat cgaacgcggg tgccgatgca
     5941 ggattggggc gcttttggac tcagtgcggc acaggaaagg catctggcaa cgcttgccgg
     6001 accacagcaa aatcaggcat tcctggcact ctggactgcg cgcgaagcgg tttccaaggc
     6061 cgatggacgc gcactctcat tgcccctgca gtgcattcaa gttgatctgt gcggcaagtc
     6121 ggcaactgtg ttggcgcctg cagccgagcc agcccggcgc tggcgcctgt ggtgtgatca
     6181 gccctctggc cgccatgtcc tggcgctggc atggcagcaa aacggcagtg acatgcagca
     6241 aatgggcacc ttgttgtaaa gtgaaccagg tccatcgggc agacatgctg gcaagcgcaa
     6301 aaaacgcgct ggcctgaacg gccaagccgg tcaacccggt actgcagacc ggtcgtgtag
     6361 cggccccagc acctgcccca actgtccagt ccacaaggtg ccgccttcaa gttgcctgaa
     6421 tttgatgcga aaatcatgcc tgagcgcttt gctgtgaaag cgcgtcaagc tacaaaacga
     6481 agagctattg agcaatcgtc cagtttgcag gtgtcatcag ggggtgacac ttgtttggcc
     6541 gttgcaccac tcacccacat caagtttttg ccatgagtta ttccgttctt tttatctgca
     6601 tgggcaatat ctgcagaagc cctaccgccg atggcgtgtt tcgccagagg gtcattgact
     6661 tcggtttgag cgatgccgtc aaggtggatt cggccggcac ccacaactac catccgaaca
     6721 gcccgcccga caagcgatcc caggcccatg cagccaagcg gggctatgac ttgtcaaaat
     6781 tgcgggcacg caagattttg catgccgact acacggcgtt tgacctgatc ctggcaatgg
     6841 accgggacaa cctggcgatg ctcaaggccg cctgcccaat ggcgcaccag cacaaactgc
     6901 gtcgtctgac cgagttttgc ctgcgcttga acagcccgga agtgcctgac ccgtattacg
     6961 gtgatgcgcg cggctttgag gtcgtgctcg atctgatcga ggacgcgtgt gacggactga
     7021 tcaggcatgt ggtgcggcaa gtcaaccaaa aaactgaaat aagctcggcg ggttagcggc
     7081 ccggacaagg tctgccaggg ccagatcggc gcacgcggta caaagtcctg cacccgcccg
     7141 gcatcgtctg gcctgacctg gcaggccagc gacatttgtg ccatgagccg caggcgggca
     7201 cgctgccagc gggatttggc agcgctcagg ctcaacccct gggctgaggc aaagtcggcc
     7261 tggctcatgc cttgcaagtc gcaggtggtg atgccttgcc ggtcgctggc gctcagctct
     7321 gccagcactc tgggcagaca ggtgctgaac tggtccaccg tctcggcagt ttctacgctg
     7381 tgtgccagat catcgggcaa ttcaaccgtg tcatggtgca gtcgcaggtg gtcgatcagc
     7441 gtgttgcgtg ccacctgaag cagccaggcg cgggcgtttt tcaggtcgca agaatgctcg
     7501 ccctggcgca gtgccttgag aaacagatct tcgacctcgg ccgggttgcc cacgctctgg
     7561 cgcaaccagc cacgtagttc cccgcaatgg aggccccagg catcggtcaa acatttcatg
     7621 ctcatcgtgt gaaattgatt agaatggact ctgaccctta cctgtattgc gctggatgct
     7681 atcaataata gatattgtgt gaccgctgat gccaatcttg gccggggcgc aaggccaggc
     7741 cgctacgata gacccatgat gccagctccc gacaacccat cccccgatat ggtcagcacg
     7801 atgctgaccc tgatcaccac ccggcaaacc attttgccca agcggctgag cgaacccggc
     7861 cccagcgcct ggcaggtcaa cgacatgttt cgcgccgccg cagccgcccc ggaccaccac
     7921 atgatcatgc cctggcgctt tgtgctggtg ccacccgacc gtcgcgcggc cctggccgat
     7981 gtgtttgccc aggccctgct ggaacgcgat gccgctgcca cacccgagca ggtggaacaa
     8041 gcctgcgaaa aagcctttcg ttcgcctttt ctggccttgg ccatcgcccg cctggggctg
     8101 tgcgagcctg acgttgaccc gctggagcgc atggtgtcgg taggtgctgc aattcagaat
     8161 tttttgctgg ttgcacacag catgggtttt ggtgccagcc tgaccagcgg gcaggcaatg
     8221 cgctcagcgg cactgcgcca gctttttcaa ctgctcgaag gtgaacaggc ggtgtgctgc
     8281 ctcaacgtcg gcaccgccaa caaacgcaag ccagcgcggt tgctccccga tgtgagcgcc
     8341 tttgtcagca gcctgtgatg cagtgcttgc aagcgccctt gtttcgcccc tgtttcattt
     8401 ccagatcatt cgtgtcaggg caccttggcc gcaaacattg caagcccaag gcagcgcgag
     8461 atgacaacga cagggagggt ctggaaatga attcaaatgc tggcaaactg cctgcaagat
     8521 aaagccatgc aacagattgc cccaaagcct gcgtcagccg atgtcgcgcc gaccggtcgc
     8581 caccctggtt tgccgcccga tgatgcgcag cgcgttgcct tgcacaacga gatgcacgcc
     8641 cgcccgagcg cacgcattcg cctgcctgcg ctgatcattg acattgccgt gctgcatgac
     8701 ggggtgacgc gcgagcttga gcacgcgcac ctgcgcagcc tgcctggtca ggccaatctg
     8761 ctgccacagc agttgcagga caacttcttg cgcctgcgcg gcgaaggttt cagtgtcaaa
     8821 tgggagcgcc acaccgagtt cacccgctac accatcgttg ggccgctgcc gtcgcaggcg
     8881 ctgctgcatg tggctgagcc agacctgatg cgcaacctgg cggtgcctgt ggcgtggctg
     8941 cagcagattc cagggcgcac cgtgtcagcg gtgcaactgg tcatgctcaa cgcgccactc
     9001 gacgatgcca aagccctgat gagccaggcc atgacctggc tcggcgcgca caccgtggtg
     9061 gcttcgttga tgggcagcaa tgcattccag gcggtcgaac ccggcggtca accagcgggc
     9121 ctgcagggtt accgtcatcc cttgggccat tcctgggcgt tgacccagtt tcaactgcaa
     9181 gacgacggct ttgaacgcat gttggtgatt gcccccgaag gcaccagcca gacccgggcc
     9241 ggccgcattg ccgggcggct gctcgaactc gaaacttacc gtctgatggc actgcgcggc
     9301 ctgcctgtag ccaaggcact cgggccaatg ctgtcgcaag ctgaaagcgc gctggccagc
     9361 atcaccgccc aactggaaaa caaaagcgcg tctgaccagc aacttcttga caccctggtg
     9421 tcgctggccg ctggtgtcga gcgcgccatg gccgaaaacg tctatcgctt tgccgccacc
     9481 caggcctact acaacatcgt tgtccagcgc atcaccgaac tgcgcgaaaa ggccattccc
     9541 ggcacccaga tgattggtga attcattcag caccgcctgt cgcccgcgat ggccacggtg
     9601 gtggcgacca actcccggct gacctcactg tcagagcggg tggcgcgcac cagcgacctg
     9661 ctgcgcaccc gggtcgatat tgccaccgag gtgcaaaacc agcagttgct ggaaaaactc
     9721 agccggggcc aggaactgca actgcgcttg cagagcacgg ttgaaggcct gtcaatagcg
     9781 gcgatctcgt attacgtgat cagcctgctg ctgtacggcg gcaaggcgct gaaggcggcc
     9841 ggggtgccga tcaaccccga aatggccgct ggggctctgg tgccggtggt gctgtggcta
     9901 gtgtggcgca ccacccagcg cattcacgca ggtttgcatg tcgggcattg agttgccgct
     9961 gcccggcgtt tgcatttgtt ttgcgttgcc agttatctaa acaagcgcct tgcattgacg
    10021 ctacgcaccc aaatccgcca gtgcaaattg gggaagatat ttctgaccac ttcgttactt
    10081 cacattgacc cgtgacaaaa ctcgtcaaag ggaacgactc agccattgcc gcgcaggctg
    10141 caagcaaagt ttttccacaa gtagctgacg cttgcggatt ctttgcctgc gcgaaaaaag
    10201 acgggggctg acctgacttc ttaaaaagct acggatgcaa gcttgcaact gccagtcgaa
    10261 ccgtaccagc aaaaaaacag gcacctgcaa cgccagcaag gcccggccag aaacagcaaa
    10321 gttccaggtc accggcttgc tgcaggccga ttgccagacc gggccagcgc cctggtgtca
    10381 atgtgaactg atggtgatgc cagcatcacc gacggcgaca tactgcacct gggccgtgat
    10441 caggccccat aaattggccg tggtggtagt gctgcgagcc gtccaggtga tgccatcagg
    10501 gctggtccgc acagcaccgg cctcgccaat ggccagaaac tgactgttgg ttgacggcac
    10561 cacaatgccg gtcaggttca ccgccgggct gagtgcggcg atgctccagg tgatgccatc
    10621 attgctcctg agcacggtgc ctgcggcacc cactgcgaca taggtgtaaa ccgatgtgaa
    10681 ttgcgtggca acaccggtga gcgcctgact ggtgccgctg atctgcgccg tccaggtcat
    10741 gccatcgctg ctggtcagca cggttccgtt ggctcccacg gcaagccaca ggccgctggc
    10801 tgcataagtg atgccgttga ggtggctgct gctgggtacg ctggccgcag cggtccaggt
    10861 ggcaccatcg cttgaatagc gaatggtgcc gttgtcgccg accgccacca ccagcgtccc
    10921 attgctggcc agcgcattga ggttgttgct ggtgcccgat accccggcgg tccaggtggc
    10981 aatgtcggtg ctgtaataaa tttgcccccc ggcaccagcc gcaatgaact tgctcagggc
    11041 gtaagtggcg gcccggaaat cgaccttcgg tccggtgctg acggcgctcc agttgatgcc
    11101 gtcggtgcct ttgtagatgg cgcctgcgtc gcccactgcg acatagtcaa ccgtgctgtc
    11161 gctggcggtg ccataagcca cactgcggat ggcggatgtg cccagcgtgc tgccgatggt
    11221 ccagctttcg ccacccggac gtggcacagt tgccaccgac ggcgtggcgg caccaccggg
    11281 gccaccatca gtgcgaccat tcatgctgaa ggcgtaggtg gtgccattgg tcaaacccgg
    11341 cagcacgtag ggggacgtca cattgatcac ccaggtgtgg ggagtgccag gggccaggga
    11401 caccgtggct gcggcctgat agcccagcca gtattcaacc cccggcgctg ctacccagct
    11461 tatcgtgact cttccgtcac cggggtcaag ttgcagtcct gcagccggtg gcggtgccgg
    11521 atcgccgccg ccaccgcacg ctgccatcag gacggcggtc aaagtgctag ccagtatcag
    11581 tcggatcgaa tgagatgaat tgaacatggt gaaccaatac aaaaagactg tcagggttgc
    11641 tggatggcgc agccaggggc cgattcaaaa attgcgtcgc atccatgggt ggcggcaatt
    11701 attacattgc agtgccaatc tgccagcggg cggtaccagg cgtgatctgg atcaagcaag
    11761 caatggcaga cgcagcctaa actcatgccg atgcgctcaa gtcgcgccag tcattgcact
    11821 gcccgccggg tacaggtttt caatgccaca ctgggtcagt accccccgcc tttgaatttg
    11881 atatgaaaat gacctctagc cttttgccag attgcgctga aagctacata ttaaatagcg
    11941 tttaacttgt ttaacggaga cacaacatga aagccttcca ggactattac cccgaaaacc
    12001 tgtcgcactg ctatggctgc ggtgcccaca acgagcacgg tcaccgcatc aagacctttt
    12061 gggacggtga cgaaactgtg acccatttca cccctgagcc gtttcacacc gccgtgcccg
    12121 gcttcactta tggtggctta ctggcatcgc tgattgactg tcatggcacc ggcaccgccg
    12181 ctgccgccat gtaccgacag gccggacgcg acatggacac gctccccggc ttcaggttcg
    12241 tcaccggttc gctgcatgtg gactacctca aacccacacc gatttcaggg ccgctggaga
    12301 ttcgcggccg ggtcaaggaa atcaagggcc gcaaggtggt ggtggaagcc accgtctatg
    12361 ccgctggcgt ggccaccgcc aggggcgaag tggtggcgat ccagatgccg gagaactttg
    12421 gcgcctgagg tcatgaaaaa gggggccgaa accccctgtt ggttacttgc caaacggcgt
    12481 tggccgcggg gtcttgtcgc ttgatggcgg cagaattggc agcatgaaac tcggctgctc
    12541 acccgtgagg ctcttcagga aagcaaccac cttggcgttt tcatccttgg tgaacgtctt
    12601 gcccaactgc agccggccca tgatgtcaac tgcttcgggc aaggtgttga cggcaccatc
    12661 gtgaaagtac gggtaggtca tttccacatt gcgcagggtc ggcaccttga agttgaagcg
    12721 atcaacttcc ttgcccgtca cggcactgcg gccttcagca ccaccggtcg atttgtaggg
    12781 tgccaccacg cccattttct ggaacgacgc accgcccact gctggaccat tgtggcaggc
    12841 cacacagcca ctttccttga acaatttgta gccagccaat tcgtcggcgc tgagcgcatc
    12901 ggccctgccg agcagccact ggtcaaagcg ggaattcggc gtgaccaggg ttttttcgaa
    12961 ctcggcaatt gccagcgtga cctggtcgat atcgaccttg tccttgccaa acacttgcct
    13021 gaattcgcgc acgtaggccg gaatcgactc cagcacacca attgccagcg tgtgggaaaa
    13081 gcccatttca ccaggattgg caatcgggcc acctgcctgc accttcaggt ctgcggcccg
    13141 accgtcccag aactgggcca cgttcagact ggcattcaac acggtcggcg cgttgatcgg
    13201 gccttgctgc catttgtctc caatggaagt cgggatgttg tcagtgccac ccatcgacag
    13261 gttgtggcag gaattgcatg agataaaccc cgacttggac aggcgcggat cgaaatacaa
    13321 ctttttgccc agttcaacca tgccgagatt gatctgcatg ggcggcttga tgggttgtat
    13381 cagttcttgt gcagccgagt agatgggtgc cagactgagg gcgctgatcg ccgctgcaag
    13441 aatgcgaagt ttgatagatc ggttccttat agtttgaaaa aattgcctga aattcgactt
    13501 ggatcaaaag aacaaaaaag atcaggcaca gacacaatgt aaccatgctg caattgcggt
    13561 taactgatat aagtcaatga tttataagat tattttttct atgaagggta taggatttac
    13621 ctttacctac aaccctgact tgccatgaaa ctgtcagcat tgcgttattt ggtggccttt
    13681 tccgaccagt tgagcttcag ccgggcggcc gaacagtgcc aggtgtctca ccccagtctc
    13741 agcgtggcct tgcagcgcct ggaagaagaa ctgggtgtca tcctgatcga tcgcagcaaa
    13801 caccatatga gcctgaccga agtgggtgag cgcatggtga cgcaggcgcg caagagcctg
    13861 gaagaagtgg ggtgcattga aactgtcgca cagtctggca aagaccctct cgacgacacc
    13921 tttcgtctgg gcatcatcca caccatcgcg ccttatctgt tgccggacct gattccagcg
    13981 ctgcaccggg tggcaccggg cctgacgctt tatatagaag agagcatgac ggccttgctg
    14041 gccgactacc tgaagtacgg cacggtcgat ggcgcaatcg tcgcgttacc gtttgacatc
    14101 cccggtattg ccacccaggc cttgtacgac gaggtgtttg aagtggcggt gcccaaggga
    14161 cacccgtggg ccgcgcgcaa gcacatcggt gcctcggaga ttggcagtga agatgtgttg
    14221 gtcctcaagg cgggcaactg ctttcgcgag caggtgctgg atgcctgccc ggagatcagc
    14281 cacaccaata cgtcaaggca tcagggtcac tccattgaga ccatccgtgc catggtggca
    14341 tcgggctatg gcatctcggt gctgcccaag agtgccttgt cagagctgta tcagtccgac
    14401 ctggtggtgt caattccgtt tgaagacccg ccgccgctca ggcgtgtggc gctggcctgg
    14461 cggcgcagtt ccgtgcatga aaaggtggtg caggtgttgc tgcaagcggt gcatgccatg
    14521 aacaagtcgg cttacaagat cgttcattag cctggggctt gcatccacct gcatcacgct
    14581 cggccattcc ggcaagcaag cggcaaaacg cggccatttt ctggattcac tatcgcttgg
    14641 cggctgatct tgcaggccct gcaaggcggt tgccgtcgca gcatgatgtc cgtggctggg
    14701 cagcagcgcc gtcgaaccac tttggtcttt cctatctttt ttggctctag cccttgtcca
    14761 tattgcgctg gcagctatgt tgataatagc tattggcgca tcgcctgatc cagtacctcc
    14821 atccagtggc gcaccggtat cccggtgcca ctgcggtgtg cgtgcccagc gccaccacac
    14881 cttcgggcgt ggcagcggcc accacgcgac cgccagcgtt gccgctgact ggcccgaggt
    14941 caagcgccca gtcggcctcg gcgattacgt ccagatcgtg ctcgatcacc accacactgt
    15001 ggccgccatc accatcaatg gcctgccacc ctggcgccgg tcccgatgcc agcgcccgtg
    15061 tatgcaccga actacagcaa taacagccag aacagccacg catcagtcaa caatgccatt
    15121 gcggcctcaa cgggcgacaa cgatattttt gccagcattg aaaagctcct cgccctgcaa
    15181 agccgtggca ttttgagtga gcaggaatac gccagtaaaa aagccgagtt gctgagccga
    15241 ctgtaatttc aagcgcctgg ttttgacggg ttggtaaaga tgaacgggca attgatctac
    15301 cgcagagact tcgaactatt ccaagaaacc tccaggcgtt gcgcggcgca caacgtaata
    15361 tgatgaacac ttgtcagcac atatacaggc tctcagaatt gttggcgcga aggggctcaa
    15421 catgaaattc tcacccagca tgaatccatt tggcatcacg actccacttt cgcttgaggt
    15481 gtggtcatga agagacgcac ggccctatgg atgctcgcct gccccgctgc aagccgggcc
    15541 gcccaccgcc gcattttggt ggtggaaagc taccacgccg aatacccttg ggaccaggac
    15601 taccgggagg ttctacaggc gttgttgggc gaacgttaca gcctggattt ctttgagatg
    15661 gataccaagc gcctaccgac agacatgcat gccggaatgg cacaaaaagc catgacctgg
    15721 attctgaacg tacgacccga gctcgtcatt ttgggtgacg atgccgccct gtattacctg
    15781 ggagagcccc tggacaagtt gaacattcca gtcgtctacc tgggcatcaa cggcaatccc
    15841 cgcaattacg gccagttgaa attcaagaac atcacaggcg tacttgagcg cccactgatc
    15901 aaacgcaata tcgtgtttat gcagagcatc atgccgtcgc tgcgcaaggt gctggttctg
    15961 ttcgacaacg acctgacctc acagcttacc tacgacgaat tgtttgacgg caggccgagc
    16021 atcatgatcg atggcataca ggccgatctg aaattgtgca agacactcga tgattggaag
    16081 cacagcattc tgtcagcacc tgcgaaaggc tatcaggcaa cgattgccgg cctgtaccac
    16141 acgctgaagc tccccaacgg aaaagtggcc gattccgata aggtgatttc gtgggcctcc
    16201 gccaacaccc cgctgccact gtttgccctg tgggggttcg ctgtcggcga gcataaggcc
    16261 attggtggcc tggtgctaag cagccgtgag caagggcgaa tggcggcaag tattgctctg
    16321 gccattctgg aacaaggggt caaaccctca gaacgccagc caatcaccgc gcgtcaggga
    16381 gatttcgtct tcagcaaagc cgagctagcc cggttcaatc tcgtacttcc aaaggacatt
    16441 gcagcacagg cgcagttgct gaactagcct gcaggctgca tgtccgatga cccgtcaccc
    16501 ggcggtcaag cgctgctggc tggctcttgc gagactttcg aaccggctgt cgagccttac
    16561 cggttttcag cttaggtttc acgtagtcac gacctttttg tggttgatcc gaaacctctc
    16621 aaaaagccat ggcaatgcac cagaaacaac ggcgttgcga atcaacattg gcagtggtgg
    16681 aatggcagtg gtcaggaaaa cgcgattttg cagatactcc cagaaagaga taccaagttt
    16741 gcggccctta atggccactt gaaattcccc caggggtgta ggcgaaattg ttcgttttcc
    16801 tcgccgttgt cattgcccct tcttgaatag cggttcccct cgcgtagcga gcacaaatca
    16861 gaggcgtgag atgcggcgat tgagagcgta tcggttcctg aatcaggtgg gggaatttca
    16921 ggtggccaca ggtgggggaa attgggtggc catcaggggt gatttggtgg gcgcgaccag
    16981 ttgcccatga ctgacctgtg gtgattggac cacccagcgg cgcaacatgt tggcgttcaa
    17041 actgtgggcc agtgcgctgg ccgccttgga gacatctggc tcgtgacagg ctgctacgac
    17101 ctggcgtttg aattcatcgc tgtaacgctc atgacggcga ccagtcgctg gtatctgagt
    17161 ttgcggagtt ggagtggaca ccatggtctt gaaaatctca gcgtcccgca aggtgacttg
    17221 cccacccgct tactttacac agagtttcgt tagcgtaacg gtttttgact cgctttcaaa
    17281 cttgacagat cgccttcggg cattgggggg aaagttgtcc ggcaactcgg ggttgtatgc
    17341 aaaaacccca gagaattccg tcactccaaa tccagccagg tcagcagggt gacctacagg
    17401 ttgatgctga catcacgggc acaaacggat ggtgcttggg cagcgccgca gtgtacgtga
    17461 gcggcgcctg ctggcgcagt ttctggcgat ctcgccacgc gcgcaggctt aaccaatgag
    17521 ggcttggagg ccacccgggt tgatgcccgg gcgcatttgc gccgcattgt g
//
LOCUS       DAOSLL010000013        17271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_3504_length_17271_cov_5.468576, whole genome shotgun sequence.
ACCESSION   DAOSLL010000013 DAOSLL010000000
VERSION     DAOSLL010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 17271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17271
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3504_length_17271_cov_5.468576"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="PLB25_01125"
     CDS             complement(<1..237)
                     /locus_tag="PLB25_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOE40227.1"
                     /translation="MSHSPDFIAPQRFSDPAAALRQARLIYDSSIAHLRAAMQAYVGG
                     ADLPGRVRACYPFVRLQTETVARRGTGNAAHLSYG"
     gene            512..2074
                     /gene="gshA"
                     /locus_tag="PLB25_01130"
     CDS             512..2074
                     /gene="gshA"
                     /locus_tag="PLB25_01130"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HOE40228.1"
                     /translation="MLKFTCCNQIRQFSEPQQAQVLRGIQRGFERECLRVDRAGQLAT
                     TAHPVALGSKLTHPWITTDYAEALLEFITPPSTDPAYPLEFLTDIHRYVAQQLGGEYL
                     WAGSMPCRIGTDSDIAIANYGSSNAARFKQVYREGLGLRYGRAMQTIAGAHYNWSLPE
                     DFWLALREVCPAEGSLQDTIDRRYFGLIRNFLRYGWLIPYLFGASPALCRSFLQGHLS
                     DLVELKAGTLHGPFATSLRMSDLGYQNHAQDKLAISFNSLSGYTQGLEAAIRTHDPYY
                     AELGVRDGDQWRQLSDSLLQTESEFYAPMRPKRIGLQGERPALALQTYGVQYIEMRLF
                     DLNPFIDIGIAPEQSLFADALLLMCLFRDSPPITSREQGENDENKHRVVTRGRQPDLQ
                     LLVHNREQPLRVLAHELFDDMAPFAAMLDSAYDSSRYASALRDLRERIDWPELTPSAQ
                     VIEAVKKHGGYFDFAMDLSKTHTRNLQSAALDSQVRHQFLTSVQQSLAAQAQLDATAQ
                     IPFEAFVANYFA"
     gene            complement(2606..3547)
                     /gene="rpoH"
                     /locus_tag="PLB25_01135"
     CDS             complement(2606..3547)
                     /gene="rpoH"
                     /locus_tag="PLB25_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HOE40229.1"
                     /translation="MTGSIGTPSTALTLANPWALVPALGNLDAYISAVNRMPMLTLEE
                     EQSFARKFRIDNDLEAAGKLVLSHLRLVVSTARQYLGYGLPHGDLIQEGNVGLMKAVK
                     RFDPDQGVRLVSYALHWIKAEIHEYILKNWRMVKMATTKAQRKLFFNLRSMKQRFRSA
                     DAMADLDSHRDTLSDSQIDSMAAELNVKREEVIEMEARLSGGDVLLDPSPSDDGEDAF
                     GPIAYLTDASHEPTAMLEAHHRDVLASDGLTQALDTLDARSRHIVEERWLKVNDDNSG
                     GMTLHDLAAVYGVSAERIRQIEVAAMKKMKKSLVEFA"
     gene            complement(4050..5012)
                     /gene="rlmJ"
                     /locus_tag="PLB25_01140"
     CDS             complement(4050..5012)
                     /gene="rlmJ"
                     /locus_tag="PLB25_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HOE40230.1"
                     /translation="MFSYRHAFHAGNHADVLKHTALIAVLRHMTQKDTALNVFDTHAG
                     AGLYRLDGDYAQTSGEAADGYLRLISRQNETLALSDNAQPATNIAAKKSPAAAPLAAA
                     LQDYLDLVASFNTSGRQQVYPGSPFIINHLLQGRDRDRLKLFELHPTDAKTLRANVAQ
                     LDAGRLVTVLQEDGFTGLKKFLPPPSRRALVLCDPSYELKTDYAQVVDMISDALKRFV
                     TGTYMVWYPIIARPEAHELPRRLKTLARREARPWLNATLTLKSSKLGHDDKGEVVRPG
                     LPASGVLVINPPHTLQATLASALKQVADMLGQSKYKAFDIETGS"
     gene            6053..11887
                     /locus_tag="PLB25_01145"
     CDS             6053..11887
                     /locus_tag="PLB25_01145"
                     /inference="COORDINATES: protein motif:HMM:NF017183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOE40231.1"
                     /translation="MARLRESEDKTEGIGMRVIRLVGLLCTWLVLALPAFAGESRFSV
                     LLDIDNNAATGCTVGTVSGTVSGIELVATTVVTTSSSGAVVTRLERQQCSGGALGAVV
                     VYDGGGWPVGLGNGTGGTAAIETSIPLALLPASGTIKAQAIASAGGGTDDATASFSIA
                     LVSGESALVVPVPLSRWLALPLSLLLVGAMAWWHRRYPAQRGLLVLVPLVLVAASGLA
                     WAASVVRDGNVGDWAGLNPVVVDAAGDAAVNADIVAVFAQKDTTNLFFRIDADVRSDA
                     ATNQAPVVSAGANQSIALSAVANLSGSASDDGLPSPAHLTTAWSTVTGPAGGSVLFGN
                     ASQLVTTASFTAPGSYALRLSADDGALIGRSDVVITVGDAGPKLVPIGDRTISLGTRL
                     RLQVVADDANVVDHLTYTLVSGPPGASLNPSPTVDWTPSDQQLGGHSFTVRVSDGVGH
                     TDQASFQVTVTNTNRPPQFAEQAAAALPRGATFARTLTATDPDPGDVLTYSLVSGPTG
                     MTLTGANLSWPTGASTPGDYPVTVKVIDAGGLFDVRRFVVTVMTAAAPVSNPDSYSVK
                     LGQTLVVNALQGVLVNDIDPMGGTLTAQKLTDPDKGSLTTFGNDGGFTYQASSVIGQT
                     FQPVVKWISHSGIAGDGGYANPRAIDVDGDGKPEIIVHRSGGAAAGTSRLVVMRGSDG
                     VELWRSGVFLPVPNDTCTYLPGQTPDDWAVADIDDSGQPAIVLSVQCTDSNVTERMVA
                     LDARTGTFKWITPALSPTFTGAALGMTIGVTPAIARLRAGEKPSVLFKRTVSGWDSAN
                     RQYCDAFQAGLTGACTVVMALDGATGGLRQTWVAAGDNEVAYWAEGGGREAWGHVAVA
                     DLTAGGKSQIVAGTAVWDVDGTLISNRLPGRGVLGLALANLDDSGQTAIVSYEIAGGY
                     ATHLVARKADGTVLWESPGETDTVHGHLTVADTDGDGKPNILVHANEALSVYNERGEV
                     VWRHSFYDGTGTRQLGEYARPVVFDLDGDGVPEIIMQTAYGIEFFDGRTGASKANVTY
                     ASLGYPQTTYGPSNRTTPLVVDIDGDGHAEVVFDIQPSTYPLDSWLVALKAQNDDWQP
                     ARPVWTQYAMHDANVTDSGHLPYPEANNFAAPATNVYANPARIAAPVDPRKREQASFT
                     YQAQADGLASNVATVNIDLLPPNRPPAFTSTPPRAYLHFGYDGSNPFVYQAYAVDPDV
                     GDTLTYSIILRGGPDTSDPNCRIGASTGKLQCNILNGGSGNFADAQPDELVIAVTDSF
                     GESAYQNIRLMPSDSLATVPGVIGQSLTSAGTALAAAGFVVGKVTEVNDVAAVGLVTA
                     QFPTTGASVPKGEWVGLTVSKGPLPVAVPLVVGQAFSAASTLLAAPGFTVVVNRVFSG
                     SDTLNTVLAQSVPAGTLLAPDAAHSITLTVSAGNGLRLAVDRSIATADQTITLTPAAV
                     DVNGAPVALPALTYTIAPLLLPYVGPLPTVAGDVITAGAGTQGAFRVTANDSVNGRTA
                     SADFVVLPPPEPGGVTHGQAYAQMATALDAIFALKPQLVEARAANDTVQMKALLQQMV
                     TLWRTVDVDDLKLSMPLVTADQFAPSLADLSALGLSATTDDVLNRQILRDAADDLRAW
                     TNGLKTPGTTMAQLRALADQFATRAARVDGMVVSEYGGIYSQREYTRLLSHRIPAFYD
                     ALFTEIALQVGMAPIEPGFPGLKHMKATLAEQLTTIAVDYVVEKIMDEGSKIYKNAKK
                     FALDTMKQVIWTAATVAFNSHLKAYVSGGDVFEVVSGASLSFRVFNEPLANQAIIEVP
                     GDFDDPELTAVMIVGPDTVAALGNAIQDLFQKMKDGFSYGLDPKNNPKKYKNGNEAKK
                     HLKEFKGKLDAIKQSVTNLQDTVDNVYQTADDVMPGCVFTSDPSCMQLIYNEGFKPVY
                     RYTPPPGFASLGGLPVPIVFIVQNQYTGLMYFGTPLFMPAPKLP"
     gene            complement(12073..12555)
                     /gene="rlmJ"
                     /locus_tag="PLB25_01150"
                     /pseudo
     CDS             complement(12073..12555)
                     /gene="rlmJ"
                     /locus_tag="PLB25_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797082.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
     gene            12876..15746
                     /locus_tag="PLB25_01155"
     CDS             12876..15746
                     /locus_tag="PLB25_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HOE40232.1"
                     /translation="MLLWGAIWGAVLGFVVDRYDVGFGIVLGALLGMFAARTLRNAVR
                     KEARSEVLKQLAAQQLVGQAQGVAALAAASGLATNTSPAAPAGLPVAASQPMDAEVSA
                     DLPDFKDTMPALAPEGSAVGTAQGSPDIPGALKPDLLTRAFAAARNWLLGGNTVVRMG
                     VLVLFVGLAFLAKYAIDNAMLPPELRLAAIGTAGIALFVFGWKLHQRQPDRLAYALTL
                     QGAGVGVLYLTVFAAFRLYQFLPAGAAFVVLGLVCAFSTAIALLQNAMLMAFIGFAGA
                     FAAPILLSTGQGDHVGLFSYYLLLGAAIVTVAWLRAWRALNLLGFFATFGVATLWGVL
                     KYQPEQLASTEPFLVAFFALYLAASLMYATRHGLMPKQAVDATLIFGLPLVAFGLQVA
                     LVRELEYAAAFSALVLGAVYLALGAWALRRRDAGVGQWLAECFVALGLGFVTLAVPLA
                     LDARWTTAVWAAEGAAVYWMGQRQARWLARLAGLALQLFAALSYLRAIDHLQTSVWPL
                     ANPGFIGAVLLAASALAIAHWSRSAATAPGSAWAQQCVALEQRLSPVLFWAGFLWWQF
                     ALGYEIERGALDPWGQWTPIFDSALQSYLEMLVWLGSAWLAHRLALPDRPQPWPIAAT
                     PAWFSLPIMLLVALYGMLASRHVFASWGWLVWPVALAAHFVTLRHLDRMLPQRWWGWV
                     HSGGVWLLVLLVGNLLVWSVQQAHLQGTAWASVILLVASVMVLLALTAPALYAPASML
                     RARWPLDRFALAYLWRAAVPLAAAVGLGAMVVAVHSDGNARPLPFVPLLNPTDITVAL
                     ALGACALWLSRVRQSDLAVPKAVLSVGWTLLLAGLAFIALNTAWLRAVHHWAGVPWNA
                     SRLYASFLVQTGYSILWTVLATGLMLLAHRRHVRRVWMGGAALLGLTVLKLFLVDLSN
                     RGGSERIVAFIAVGALMLVVGYFAPIPPAAAAIPTVADKEPL"
     gene            15743..17149
                     /locus_tag="PLB25_01160"
     CDS             15743..17149
                     /locus_tag="PLB25_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3999 family protein"
                     /protein_id="HOE40233.1"
                     /translation="MRVDRWCAMLLAGAMAAAVAADAPQLAEFAWRGTLVLPAGASLA
                     RVDLPVQAMLQMQSSAAHDLRVFNGTGAVVPYAVLGGADLTRPAPAATTTVYKAYPLF
                     AANTTGKARRGAVSVQVDTAGPHGSAWVRWDTTDRTADLPDPGAQPLQAALFDTRSEQ
                     KTVDALLLAVNLPRNTLVPVTVATSTNLKDWTVHETKGPLFQFDGADAPVNSALELRQ
                     PLQLKGLYLRLTWTGQEGVKVQTLTARVAGTQTAPTPLRAPLPPGTPDGSSRLSWNLS
                     FAAPISALHLQALQDNSLVPVRILGRNDRAQPWRTLASAVVYRLDREGTGSSNAPTPL
                     HGASVRALQVEASQGVALPAGALQATVEFSPLQVVFLASGTGPFTLAVGRSHTPSAAV
                     EASLLGSVTSAGLGELPLASVGSTHIQPETALAGPVDWLPEGASLRNVLLWVVLGVGV
                     LVLGGVAYSLLRQLGARR"
BASE COUNT         3102 a         5085 c         5526 g         3558 t
ORIGIN      
        1 cccgtagctc aggtgcgcgg cattgccagt tccacggcgc gccacggttt cggtctgcag
       61 ccgcacaaac gggtagcagg cacgcacccg gccaggtaaa tcagcaccgc ccacataggc
      121 ctgcatggcg gcacgcaggt gcgcgatgct gctgtcgtaa atcagccggg cttggcgcag
      181 cgcggcggcc gggtcgctaa accgctgggg ggcaatgaaa tcgggcgaat gggacatgag
      241 cggcattgtt tcacgtcttc acgacagtgc tgtgacgcag cgcccgccgg gtgcgcagct
      301 aaactggcat actgccaccc tcgaaccagc cgacaacggt tggctttggc aaacggcagt
      361 gatgccagcc atgaggcaat ccctgcactg ccttgccaga tcaccgcttg cgccgcgatg
      421 acagttgtcg agtcaagctc gatgcgcggt ggctggccgc aatcgcaggc attcatgaca
      481 aactcccgat ccacgccacg ctgactaacc catgcttaaa ttcacttgct gcaaccagat
      541 ccgccagttt tcagaacccc agcaggcgca ggtgctgcgc ggcattcagc gcggctttga
      601 gcgtgaatgc ctgcgtgttg atcgcgccgg acaactggcc accacagcgc atccagtggc
      661 gctggggtcc aagctcacgc acccctggat caccaccgac tatgccgagg cgttgctgga
      721 attcatcacc ccaccatcga ccgaccctgc ctatccactg gaatttttga ccgatattca
      781 ccgttatgtg gcgcagcagc ttggcggtga gtatctgtgg gcgggctcca tgccctgccg
      841 gattggcacc gatagcgaca ttgccattgc caactacggg tcatccaacg ctgcgaggtt
      901 caaacaggtg taccgcgaag gcctcgggct gcgctatggt cgcgccatgc aaaccatcgc
      961 cggggcgcac tacaactggt cgctaccgga agatttctgg ctcgccctgc gcgaagtctg
     1021 cccggcagaa ggcagcctgc aagacaccat tgatcgacgc tatttcggtc tgattcgcaa
     1081 ctttttgcgt tacggctggc tgattcccta cctgtttggt gcctcacccg cgctttgccg
     1141 gtcgttcttg caaggccact tgtcggacct ggtcgaactc aaggccggca cgctgcacgg
     1201 gccgtttgcg accagcctgc gcatgagtga cctgggctac cagaaccatg cgcaggacaa
     1261 gctggcgatc agcttcaatt cactgtctgg ctacacccag ggtctggaag ctgcgattcg
     1321 cacgcacgac ccctactatg ccgaactcgg cgtgcgcgac ggcgaccagt ggcgccaact
     1381 cagcgacagc ctgctgcaaa ccgagagcga gttttatgcg ccgatgcgcc cgaaacgcat
     1441 tggcctgcaa ggtgaacgcc cggcgctggc cttgcaaacc tatggcgtgc agtacatcga
     1501 gatgcggctg tttgacctta acccgttcat tgacatcggc atagcccccg agcaaagcct
     1561 gtttgcagac gcgctgttgc tgatgtgtct gttccgggac agtccgccaa ttaccagccg
     1621 cgagcagggc gaaaacgatg aaaacaagca ccgtgtcgtc acccggggtc gccagcctga
     1681 cctgcaactg ctggtgcata accgcgaaca gccgttgcgc gtgctggcgc acgagttgtt
     1741 tgacgacatg gcgccgtttg ccgcgatgct cgacagcgcc tatgacagta gccgctatgc
     1801 aagcgcgctg cgtgatttgc gcgagcgtat cgactggccc gagctcaccc cttcagcgca
     1861 agtgattgaa gcggtgaaaa agcacggcgg ttactttgac tttgccatgg atttgtccaa
     1921 aacccacacg cgaaacctgc agtctgcggc cctggattca caggtcaggc accaattcct
     1981 caccagtgtg cagcagtcgc tggcagcaca agcgcaactc gacgcgacag cccagatacc
     2041 ttttgaggca tttgtcgcca attactttgc ctgacggcag gctgatcttg tgcgggcatg
     2101 gatttgccag cagcaagtgc ctggccgctg gtgctcggtt actcagcgtg cctgatggtc
     2161 agatctgggt agcggtctga gctgaattgg ttcgatggct cgatggttcg ggtcaatgtc
     2221 ctgtctgcta ttttttatct agcttgcagc gcacagtgac aaagggctgc agccctaaat
     2281 gccatcaacc caccggagcc tcactggctg ttgagcgcca gcgccttgat cacaccgccg
     2341 aactatgtgg tgtcgccctt gatgcgatcg gccaggcctt caggcgatga tctcaccagc
     2401 ttgaccgctt tggcttggcc agcccggagt ttgcaggcca ttttgaccgc cagcccaagt
     2461 ggtgattgcg caggcagcta ttaaaactat agtgtcgcgg cgactgaaca ttggtagcga
     2521 tggggattga tgtgggcaaa aaaaagccca agtgcgcgtg gcgcagcact tggggtgggt
     2581 gtcgggcgcg accggcccgc cgggttcagg cgaactcaac cagcgatttc ttcatcttct
     2641 tcatggcggc aacctcgatc tggcggatgc gctcggcgct aacaccatac acggcagcca
     2701 agtcgtgcag cgtcatgcca cccgagttgt cgtcgttcac cttgagccag cgctcctcca
     2761 caatgtggcg gctgcgtgcg tccagcgtgt caagcgcctg tgtcaggcca tcactggcca
     2821 gcacatcacg gtgatgcgct tcgagcatgg cggttggttc atgagaggca tcggtcaggt
     2881 aggcaattgg tccgaaggca tcttcgccgt cgtcggacgg gctcgggtca agcagaacgt
     2941 caccacccga caggcgcgct tccatctcga tgacttcctc gcgcttgacg ttgagttcgg
     3001 ccgccattga atcaatctgg ctgtcgctca gggtgtcacg gtggctatcg aggtcggcca
     3061 tcgcatcggc actgcgaaag cgctgcttca ttgagcgcag gttgaaaaac agcttgcgct
     3121 gcgccttggt ggtggccatt ttgaccatgc gccagttttt cagaatgtat tcgtgaatct
     3181 cggccttgat ccagtgcagc gcgtagctga ccaggcgcac gccctgatcg gggtcaaagc
     3241 gcttgaccgc cttcatcagc ccgacattgc cttcctgaat caggtcgccg tgcggcaagc
     3301 catagcccag gtactggcgg gctgtggaca ccaccagccg caggtgagac aacaccagct
     3361 tgccggccgc ttcaaggtcg ttgtcgatgc gaaatttgcg tgcaaacgac tgctcttctt
     3421 ccagtgtcag catcggcatg cggttgaccg ctgaaatgta agcgtcaaga ttgcccaagg
     3481 ctggcaccag tgcccagggg ttggccaggg tcagcgcagt gcttggtgtg ccaattgatc
     3541 cagtcattca agtactcctg ttgcttgatg cgtgaatatt agcactctct tgggtagagt
     3601 gccaacaact tgcgtcaagt cccgttttgg gcctgggcat ctgctgtgtc gtttttgcac
     3661 caggcccggt tggcgtgcgt tatctgcaag gcgggcgaag cgccgcagtg ggtttgcggc
     3721 gtgtcattgg cagccctgat gtcactgcca cctggcccgc tgttgaacca gacttgcctg
     3781 gtgaagcgcc agccatctgc atgtgggcct tgccactgat cgtgcttgcg ccagcggctt
     3841 atgccgtttc taaatcacgt gttcaggctt cctggcgcag acattgcaaa agcacggcaa
     3901 ggtgagacaa caaagatctg gatgacttcg aaatggcatg actcgggcct agcgggctat
     3961 cacccggtag gtgaaactca tcgagtctga aaatcaaatg tgcacaagga cttgcagttg
     4021 aaacaagcgg ttaactgaga aaaccagggt caactgccgg tctcgatgtc aaaagccttg
     4081 tatttggact gccccagcat gtcagcgact tgcttgagcg cgctggcaag cgttgcctgc
     4141 aaggtgtgcg gcggattgat caccagcacg ccactggcgg gcagtcccgg gcgcaccact
     4201 tcgcccttgt cgtcgtgccc gagtttgctg gatttgaggg tcagcgtggc gttcagccat
     4261 ggccgggctt cgcgcctggc cagggtcttg agcctgcgcg gcaactcgtg ggcctccggg
     4321 cgcgcaatga tcgggtacca caccatgtag gtgccagtga caaaccgctt tagcgcgtcg
     4381 ctgatcatgt ccaccacctg agcgtaatcg gtcttgagct catagctcgg gtcgcacagc
     4441 accagggcac ggcgcgacgg cggcggcaaa aacttcttca ggccggtaaa accatcctct
     4501 tgcagcaccg tgaccagtcg cccggcatca agctgggcca cgttggcgcg cagggtcttg
     4561 gcatcggtcg ggtgcagctc aaacagcttg agccggtcgc gatcacggcc ttgcagcagg
     4621 tgattgatga tgaaggggga gccggggtaa acctgctgtc tgccgctggt gttgaaactg
     4681 gcaaccaggt ccagatagtc ttgcagggcg gctgccaagg gcgccgcagc gggtgacttt
     4741 tttgctgcga tatttgtagc tggctgcgca ttgtcagaaa gggctagagt ctcattttgc
     4801 ctggaaataa ggcgcagata accatctgct gcttcgccac tggtctgcgc atagtcgcca
     4861 tcgagccggt acaaacccgc gcccgcgtgg gtgtcaaaca cattcagggc ggtgtctttt
     4921 tgggtcatgt ggcgcagcac ggcgatcagc gcggtgtgtt tcaggacatc ggcatggttg
     4981 ccggcatgga aggcgtggcg gtaactgaac atacgcggca tgttaaccgc cactttgaac
     5041 atcggtgtca gcgcgcttgg ttctcagcag cgaaatacaa cttgatccga cagtcaggcg
     5101 aggcagcagg ggccaccagg gagtgcacca ggacaatttg gttcttcctc caagttggtg
     5161 gtttgccgtc attaccgcga aggcgggaat ccaatgcttc attaaattcg atgcaatcgc
     5221 ggcaattgat aacagaggtg caatgtcctg agaacaaatt gatgttgagg tgaactggca
     5281 tccaggttgg aagtcagggc gacagcctgg ggctgccaat aaaaggccgg agatcgactg
     5341 ctgccgctgt tcatcctggg ctggctggaa ttactgctcg agagtttcaa agtcaacatg
     5401 cagatcgtct tgccagtccc aacggttggt cagcggccga actgggtact gtgtatcgac
     5461 ttcctgtggg cagcctgaaa ggcatgatta tgaatggtgg tctgccgtca atcgggggcc
     5521 acaagttcgc gcccagaacc cggtcagata cgcggtcccg tccattgatg cagcgcagat
     5581 agcccaagag tcagcgtgat tgtgttcgaa agcgggtttt accgcagcgc acaaagtggc
     5641 tgatcggcgc caggatgacc ctgcccccaa tgccgtcaat cgtttgcagg catcgggcag
     5701 atacttggtg caatgttgcc aacgcacaac gaaaaaacaa tcaagacaag atttgtccga
     5761 taaaactgag aaaaagcgtt gaaactgtta gcgaaatttt tgcaaacgcc tgatcgccaa
     5821 aaaggaacag tgctgcggtt aaaaaaaaga gcgtcttgga agctgcgcca gttacaggtg
     5881 tctcttaaca cacttcaacc gagggctggc aattgaaaca atcggaatgt gagttttagt
     5941 ttatgggcag tgctgggtag ccatccccat cgtaggctgt gaaacatacg gcagggtcgt
     6001 tcggctgcca cgcttcagac actgcgccaa catggcttcg caggcgggcc aaatggctcg
     6061 attgcgtgaa tcggaagata agactgaggg tattggcatg agagttattc gcctggttgg
     6121 gttgctctgc acctggttgg tactggcact gccagcattt gcgggcgagt cccgcttttc
     6181 cgtgttgctc gatatcgata acaacgcggc aacgggatgt accgtcggca ctgtcagcgg
     6241 tactgtgtca ggtatcgagc tggtagcgac gacagtcgtg acgacttcgt ccagcggggc
     6301 ggtggtgacc cgccttgagc ggcagcagtg cagtggcggc gcactcggtg cggtggttgt
     6361 ctacgacggt ggtggctggc cagtgggcct gggcaatggc actggcggca cggcagcaat
     6421 cgaaacctct atacctctgg cattgttgcc tgcaagcggc acgatcaaag cacaggcaat
     6481 cgcgagcgct gggggtggta ccgacgacgc gacggcatcg ttctcgattg cgctcgtttc
     6541 tggcgagtcg gctctggtgg tgccggtacc gctgtcgcgc tggcttgccc tgccgctttc
     6601 gctattgttg gtcggtgcga tggcctggtg gcaccgacgt tacccggcgc agcgaggcct
     6661 gctggtgctg gtgccgttgg tgctggtcgc tgcatcgggg ttggcgtggg cggccagcgt
     6721 agtgcgcgac ggcaatgtcg gtgactgggc cggattgaat ccggtggtgg tggatgctgc
     6781 tggcgatgcg gcggtgaacg ccgacatcgt ggcggtattt gcgcaaaagg acacaacgaa
     6841 tctttttttc cgcatcgacg ccgacgtgcg cagcgatgcg gcgacaaacc aggcaccggt
     6901 ggtcagtgcc ggggcaaacc agagcatcgc cttgtcagcg gtggcaaacc tgagcggctc
     6961 ggcttcggac gacgggctgc cgtcaccggc gcacctcacc accgcttgga gcaccgtgac
     7021 cggtccggct ggtggtagtg tgctgttcgg caacgccagc cagctcgtta ccaccgccag
     7081 cttcactgcc cccgggagtt acgcgttgcg gcttagcgcc gatgatggcg cactcattgg
     7141 tcgatcggat gtggtgatca ccgtcggcga cgctggaccg aagctagtgc cgattggcga
     7201 tcgcaccatt tcgttgggca ctcgtctgcg actgcaggtg gtggcggatg atgcgaatgt
     7261 cgttgatcat ctgacctaca cgctggtctc agggccgcct ggcgcgagtc tcaatccgag
     7321 cccgactgtc gattggacac cgagcgatca gcaactcggc ggccacagtt tcaccgttcg
     7381 cgtaagcgat ggtgttggcc acaccgatca ggccagcttc caggtcactg tcaccaatac
     7441 caatcgaccg ccacagttcg cggagcaggc cgccgccgcg ttgccgcgcg gggccacctt
     7501 tgcccgcacg ctcacggcga ctgacccgga cccaggcgac gtgttgacgt actctctggt
     7561 gtccggtccg accgggatga cgctgaccgg tgccaacctg tcgtggccaa ccggagcatc
     7621 aacacctggt gactatccgg tcacggtgaa agtcatcgat gcaggtggtc ttttcgatgt
     7681 ccggcggttc gtggtcacgg tgatgaccgc tgcagctccg gtatccaacc ccgacagtta
     7741 cagcgtgaag ctgggccaga cgctggtggt gaatgcgttg cagggtgtac ttgtcaacga
     7801 catcgacccg atgggtggca cactgaccgc gcagaagctc accgaccccg acaagggcag
     7861 tcttacgacc tttggcaatg atggtggttt cacctaccag gcatcgagcg tcatcggcca
     7921 gaccttccag ccggtggtga aatggatcag ccacagcggt atcgctggcg atggcggcta
     7981 cgccaaccct cgcgccatcg acgttgatgg cgacggcaag ccggagatca ttgtccaccg
     8041 tagcggcggc gcggcggctg gcacttcgcg acttgtagtg atgcgtggca gcgacggcgt
     8101 ggaactttgg cgctcgggag tctttctacc ggtgccgaac gacacgtgca cgtaccttcc
     8161 aggccagaca cccgatgact gggcggtcgc tgacattgac gacagcggcc aaccggccat
     8221 cgtgctttcg gtgcagtgca ctgactcgaa cgtcacggaa cgtatggtcg cgctggatgc
     8281 aaggaccggc acattcaagt ggatcactcc ggcgctctcg ccgacgttca ccggcgctgc
     8341 tctcgggatg actataggcg tgaccccggc cattgcccgg ctgcgggcgg gcgagaagcc
     8401 gagcgtgctg ttcaagcgga ccgtgtcggg atgggacagc gccaaccggc aatactgcga
     8461 cgcgttccaa gcgggactca ccggtgcctg caccgtggtg atggcgctcg acggcgccac
     8521 cggtgggctg cggcagacgt gggttgcggc cggcgacaac gaggttgcct actgggcaga
     8581 gggcggcggt agggaggcgt gggggcacgt cgcggttgcc gatctcaccg ctggcggcaa
     8641 gtcgcagatc gtcgccggca ccgcggtgtg ggatgtcgac ggcacgctga tctcaaaccg
     8701 gctgcccggg cgcggcgtgc tgggcctggc gctggccaat ctcgacgatt ccggtcagac
     8761 cgccatcgtc agctatgaaa tcgcgggcgg atacgccacc cacctcgtcg cgcgcaaggc
     8821 cgacggcacc gtgctttggg agtcgccggg cgagaccgac accgtgcatg ggcacctgac
     8881 ggtggccgac accgacggcg acggcaagcc gaacatccta gtgcacgcga acgaggcgct
     8941 gtccgtctac aacgagcggg gagaggtcgt ctggcggcac agcttctatg atggcaccgg
     9001 tactcgccag ctcggcgaat acgcacgccc ggtggttttc gatctcgacg gtgacggcgt
     9061 gcccgagatc atcatgcaga ccgcctatgg catcgagttc ttcgacggca ggaccggtgc
     9121 cagcaaggcc aacgtaacct acgcctcgct gggctatccc cagaccacct acgggccttc
     9181 caaccgaacc acaccactgg tggtcgacat cgatggcgac gggcacgcgg aagtggtctt
     9241 cgacatccag ccttcgacat acccgctgga cagttggctg gtcgcgctca aggcgcagaa
     9301 cgacgactgg cagccggcgc ggccggtgtg gacccagtac gcgatgcacg atgccaacgt
     9361 caccgattcc ggccacttac cgtatccgga ggcgaacaac ttcgccgccc ccgcgaccaa
     9421 cgtctacgcc aacccggcac ggatcgccgc accggtggac ccgcggaagc gcgagcaggc
     9481 gagcttcacc taccaggcgc aggccgacgg gctcgcatcg aacgtcgcta ccgtcaacat
     9541 cgatctcctg ccgcccaacc ggccgccggc gtttacctcg acgccgccac gggcctatct
     9601 gcacttcggc tacgacggga gcaatccgtt cgtctaccag gcgtatgcgg tcgaccccga
     9661 tgtcggcgac acgctgacct attcgatcat cttgcgcggc ggccccgaca cctccgatcc
     9721 aaactgcagg atcggcgctt cgaccggcaa gctccagtgc aacatcctca acggcggcag
     9781 cggcaatttc gccgatgccc aaccggacga actggtgatc gcggtgacag atagctttgg
     9841 tgaatcggcg taccagaaca ttcgcctgat gccgagtgac agcttggcaa cggtacccgg
     9901 cgttatcggc cagtcgttga cgagcgctgg cacggcgctc gcggcagcgg gtttcgtcgt
     9961 cggcaaggtg accgaggtca acgacgtggc ggcggtgggg ctggtgaccg cgcagtttcc
    10021 gacgaccggg gcaagcgtgc cgaagggcga gtgggtgggt ttgacagttt ccaagggtcc
    10081 gctgccggtg gcggtgcctt tggtcgtcgg tcaggcgttc tcggctgcga gcacgctgtt
    10141 ggcagcgcca ggtttcacag tggtggtcaa ccgcgtgttc tctggctcag acaccctcaa
    10201 cacggtactg gcgcaatcgg tgcctgctgg gacactgctc gcgccggatg ccgcacactc
    10261 gatcacgctg acggtgtcgg ctggcaacgg gctgcggctc gcagttgacc gctcgatcgc
    10321 cacggctgac caaaccatta cgctcactcc ggcggcggtc gatgtcaacg gtgcgccagt
    10381 tgcattgcca gcgctgacat acacaattgc gccgctgctg ctgccttatg tcggcccgct
    10441 gcccactgtc gctggtgatg tcatcacggc aggggctggg acgcagggcg cgttccgggt
    10501 gaccgcgaac gattcagtta acggccgcac cgccagcgcc gatttcgtgg tgctgccgcc
    10561 gccagagccg ggcggggtaa cacacggcca ggcatatgca cagatggcga cggcgctgga
    10621 cgcgattttc gcgctgaagc cacaattggt cgaggcccgt gctgccaacg acacggtgca
    10681 gatgaaggcg ctgctacagc agatggtgac gctgtggcgg acggtggatg ttgacgacct
    10741 caagctatcg atgccgctgg tcactgccga tcaatttgcc ccgtcgcttg ccgatctgtc
    10801 cgcgctggga ctgtcggcga ccactgacga tgtcttgaac aggcagattt tgcgcgacgc
    10861 agcggatgac ctgcgggcgt ggaccaatgg tctgaagaca ccaggcacca ccatggcgca
    10921 actcagggcg ctggccgatc agttcgccac ccgcgccgct cgtgtggatg ggatggtggt
    10981 gagtgagtac ggtggcattt acagccagcg cgaatacacg cgcctgctgt cgcaccggat
    11041 ccccgctttc tacgatgcat tgttcaccga aatcgcgctg caggtgggaa tggcgccaat
    11101 tgaaccaggc tttcccggac ttaagcacat gaaggcgacg ctggccgagc agttgacgac
    11161 aatcgcggta gattacgtgg tcgaaaagat catggatgag ggcagcaaga tctacaagaa
    11221 cgccaagaaa ttcgcgctcg acacgatgaa gcaggtgatc tggacggcgg ctacggtggc
    11281 gttcaactca cacctcaagg cttatgtttc tgggggcgac gtgttcgagg tggtttccgg
    11341 tgcatcgctg tcgttccgcg tgttcaacga gccactggcc aaccaagcga tcattgaggt
    11401 gcctggtgat tttgacgatc cggagttgac tgcggtgatg atcgtggggc ccgacaccgt
    11461 ggctgccctc ggcaacgcca tccaagacct cttccagaaa atgaaggatg gctttagcta
    11521 cggtctcgat cccaaaaaca atccaaagaa gtacaagaac ggcaacgagg ccaagaagca
    11581 tctgaaggaa ttcaagggca agctcgacgc gatcaaacag tcggtgacca atctacagga
    11641 tacggtggac aacgtttatc agaccgccga cgacgttatg ccgggttgcg ttttcacctc
    11701 cgatccgtcc tgcatgcaac tcatctacaa cgagggcttc aagccagtct atcgctacac
    11761 cccgccgccg ggctttgcga gcctgggcgg gctgccggtg ccgattgtct ttatcgtgca
    11821 gaaccagtac accggtctca tgtacttcgg cacgccattg ttcatgccgg caccgaagtt
    11881 gccctgaacg ggagctggct aggaaccgat tggacttatg ccccgacaac gagaagctac
    11941 cccagcgagg ccagcttgcg ccagactggc agacccatat tatggttgtg ggtcaatagg
    12001 caggtgcaag atgggccgct gtggtcaggt cgcagctgac gcgccaatgg tccgacatac
    12061 tgtttggggt ctgattcggg ctgagcgtct ggccagggtc ttggcatcgg tcgggtgcag
    12121 ctcaaacagc ttgagccggt cgcgatcgcg gccttgcagc aggtggttga tgatgaaggg
    12181 ggagccgggg taaacctgct gtctgccgct ggtgttgaaa ctggcaacca ggtccagata
    12241 gtcttgcagg gcggctgcca agggcgccgc agcgggtgac ttttttgctg cgatatttgt
    12301 agctggctgc gcattgtcag aaagggctag agtctcattt tgcctggaaa taaggcgcag
    12361 ataaccatct gctgcttcgc cactggtctg cgcatagtcg ccatcgagcc ggtacaaacc
    12421 cgcgcccgcg tgggtgtcaa acacattcag ggcggtgtct ttttgggtca tgtggcgcag
    12481 cacggcgatc agcgcggtgt gtttcaggac atcggcatgg ttgccggcat ggaaggcgtg
    12541 gcggtaactg aacatacgcg gcatgttaac cgccactttg aagatcggtg tcaacgcgcg
    12601 ttgccagttg cgcttttgaa cctgaacaca caggtctttg atctcgatgc caacggacct
    12661 ggcgctgctt gatcaattga gggcagcgtt tgctcgatgg tcatggtttg tccggcacgg
    12721 cctcaggatg gcgggaatgc tggcatcgac caactgttgg gattgacatc aaatccgttg
    12781 cggccgaatt gaaatgatca gacaacatgg gctagactgc cccgggctta tgctgctggc
    12841 ttaaaggctt gaccggtcca atcgaggagt atgcgatgct gctatggggt gcaatctggg
    12901 gtgcggtgct gggcttcgtg gttgatcggt acgatgtcgg ttttggcatc gtgctgggag
    12961 ccctgctggg gatgtttgcc gcgcggaccc tgcgcaatgc agtgcgcaag gaggcccgca
    13021 gcgaagtgct caaacaactg gcagcgcagc agcttgtcgg tcaggcccaa ggcgtggcag
    13081 cccttgccgc agcgagcggg ttggcaacca acacgtcgcc tgccgccccc gctggcttgc
    13141 cggtagccgc aagccagccc atggatgccg aagtgtctgc cgacctgcca gatttcaagg
    13201 acaccatgcc agctcttgcc cccgaaggca gcgccgtggg cactgcgcaa gggtcgcctg
    13261 acatccccgg cgcattgaag cccgacctgc tgacccgcgc ctttgctgct gcgcgcaact
    13321 ggctgctggg tggcaacacc gtggtgcgca tgggtgtgct ggtgctgttt gtcgggctgg
    13381 ccttcttggc caagtacgcc atcgacaacg ccatgctgcc gcccgaattg cggttggccg
    13441 ccattggcac ggcaggcatt gcgttgtttg tttttggctg gaaattgcac cagcgccagc
    13501 cagaccggct ggcctatgcg ttgaccttgc agggcgcggg ggtcggggtg ctgtatctca
    13561 cggtgtttgc cgcctttcgc ctctaccagt ttttgccagc cggtgcagcc tttgtcgtgc
    13621 tgggtctggt gtgtgcgttt tctaccgcca ttgcgctgct gcaaaacgcc atgctgatgg
    13681 ccttcatcgg ctttgccggt gcctttgccg cacccatact gctgtccacc ggtcagggcg
    13741 accatgtcgg gctgttttcc tactacctgc tgttgggcgc tgccatcgtt accgtggcct
    13801 ggctgcgggc ctggcgcgcg ctgaacctgc tgggtttttt tgccaccttt ggcgtcgcga
    13861 cgctatgggg tgtgctgaag taccagcccg agcaactggc cagcaccgaa ccgttcctgg
    13921 tggctttctt tgcgctgtat ctggcagcca gcctgatgta cgccacacgc cacggtttga
    13981 tgcccaaaca ggcggtcgat gcaaccctga tctttggtct gccgctggtg gcttttggcc
    14041 tgcaggtggc tttggtgcgt gagcttgagt atgccgctgc gttctctgca ttggtgttgg
    14101 gggcggtcta cctggcgctg ggcgcttggg cactcaggcg gcgcgacgct ggcgtcggcc
    14161 aatggctggc cgagtgtttt gtcgcattgg ggctgggctt tgtgacactg gccgtgccgc
    14221 tggcactgga tgcacgctgg accactgccg tctgggctgc cgaaggtgcg gcggtgtact
    14281 ggatgggtca gcgtcaggcg cgctggctgg cgcggctggc ggggctggca ttgcaactgt
    14341 ttgcagcctt aagttacttg cgggccatcg atcacctcca gacatcggtc tggccgcttg
    14401 ccaacccggg tttcattggc gcggtgttgc tggccgcgtc ggcgctggcc attgcgcact
    14461 ggtcgcgcag tgcggcaaca gcgccggggt cggcttgggc gcaacagtgt gtggcgctgg
    14521 agcaacggct ttcgccggtg ctattctggg ccggattctt gtggtggcag ttcgcgctgg
    14581 ggtatgagat tgagcgtggg gcgcttgatc cctggggcca atggacaccg attttcgaca
    14641 gtgcgttgca gtcctatctg gaaatgctgg tctggctcgg cagcgcctgg ctggcccatc
    14701 gtctggcgct gccagatcgg ccacagcctt ggccgattgc cgccacgccg gcctggtttt
    14761 ccttgcccat catgctgctg gtggccctgt acggcatgct ggcgtcacgg catgtgttcg
    14821 cgtcctgggg ttggctggtc tggccggtgg cactggctgc gcactttgtc acgctgcgcc
    14881 atcttgaccg catgctgccg cagcgctggt ggggctgggt gcacagcggc ggcgtgtggc
    14941 tgcttgtttt gctggttggc aacctgctgg tctggagtgt gcagcaagcg catttgcagg
    15001 gcaccgcctg ggccagcgtg attctgctgg tggccagtgt gatggtgctg ctggcactga
    15061 cggcacccgc gctctatgcg ccagccagca tgttgcgcgc gcgctggccg ctcgaccgtt
    15121 ttgccttggc ctatctgtgg cgcgctgctg taccgctggc ggcggccgtc gggctggggg
    15181 ccatggtggt ggcggtgcat tcggacggca atgcaagacc cttgcccttt gtgccattgc
    15241 tcaatcccac cgacatcacg gtggcgctgg cgctcggggc ctgcgctttg tggttaagcc
    15301 gcgtgcgcca aagcgatttg gcagtaccca aggccgtgct gtctgtgggc tggaccttgc
    15361 tgctggcagg tctggccttc attgcgctca acaccgcctg gctgcgggcg gtacaccact
    15421 gggctggcgt gccgtggaac gccagccgcc tgtatgcatc ctttctggtg caaaccggct
    15481 actcgatttt gtggaccgtt ctggccaccg ggctgatgct gctggcgcac cgccgccacg
    15541 tgcgccgggt ctggatgggg ggtgccgcct tgctcgggct gacggtgctc aagctgtttc
    15601 tggtggacct ttccaaccgg ggcggttcgg aacgtatcgt cgcgttcatc gcagtgggcg
    15661 cgctgatgct ggtggtcggc tactttgcac ccatcccgcc cgcagcggct gcaataccta
    15721 ctgttgcgga taaggaacca ttatgagagt tgaccgttgg tgtgccatgc tgttggccgg
    15781 tgccatggca gcagccgttg ccgccgatgc gccgcagctt gccgaatttg cctggcgcgg
    15841 cacattggtg ctgcctgccg gtgccagcct ggcacgggtg gacctgccgg tgcaggccat
    15901 gctgcagatg caaagcagcg cggcgcacga tttgcgggtg ttcaatggca ccggcgcggt
    15961 ggtgccctac gcggtactgg gtggtgcaga cctgacccgc cctgcgcccg cagccacgac
    16021 caccgtttac aaggcgtacc cgttgtttgc tgccaacacg acgggcaaag ccaggcgggg
    16081 cgcggtgtcg gtgcaggtgg acactgccgg cccacatggc agcgcctggg tgcgctggga
    16141 caccacggat agaaccgccg acctgccgga ccccggcgcg cagccactgc aagcggccct
    16201 gttcgacacg cgatccgagc aaaagacagt cgatgcattg ctgcttgccg tgaacttgcc
    16261 ccgcaacacg ctggtgcccg ttacggtggc caccagcacc aatctgaaag actggacggt
    16321 gcatgaaacc aaggggccac tgttccagtt tgacggtgcc gatgcgccag tcaattccgc
    16381 cctggaattg cgccagccgc tgcagttgaa ggggctctac ctgcgtctga cctggaccgg
    16441 tcaggagggg gtcaaggtgc aaacactgac cgcaagggtg gccggaaccc agactgctcc
    16501 aacaccgctg cgcgcgcctt tgccgccggg cacgccggac ggtagcagca ggctgagctg
    16561 gaacctgtcg tttgcggcac cgatcagcgc gctgcacctg caagcgttgc aggacaacag
    16621 cctggtgccg gtacgcatac tggggcgcaa tgatcgtgcc cagccgtggc gaacactggc
    16681 ctcggcggtg gtttatcggc ttgatcgcga aggcaccggc agcagcaatg cacccacgcc
    16741 gttgcacggt gcctcggttc gtgccttgca ggtcgaagcc agccaaggtg tcgccttgcc
    16801 tgcaggtgca ctgcaggcaa ccgtcgaatt ttcgccgctg caggtggtgt ttctggcttc
    16861 aggcactggt ccgttcaccc ttgctgttgg tcgcagtcat acgccatcgg cagcggtaga
    16921 ggcatctctg ctgggctcgg tcacctcggc tggtttgggc gaattgccgt tagccagcgt
    16981 ggggagcact cacatccagc ctgaaacggc gcttgcagga ccagtggatt ggctgcctga
    17041 aggcgcttcg ttgcgcaatg tcttgctgtg ggtggtgctg ggcgttggcg tgctggtact
    17101 gggtggcgtg gcgtattcgt tgttgcgcca actcggagcc cgccgttagt tgcctgtcag
    17161 acttatggtg catcggctgc gaaacggccg ttgcgcgcca attttgcgcc ggttttttga
    17221 tccatcgccg ggcgttcgtg ttgcacattc ggtacaaact ggcctgtctg g
//
LOCUS       DAOSLL010000014        17013 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_3566_length_17013_cov_4.506664, whole genome shotgun sequence.
ACCESSION   DAOSLL010000014 DAOSLL010000000
VERSION     DAOSLL010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 17013)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17013)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17013
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3566_length_17013_cov_4.506664"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..609)
                     /locus_tag="PLB25_01165"
     CDS             complement(<1..609)
                     /locus_tag="PLB25_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE40234.1"
                     /translation="MKRGFFTIMAAQFFSSLADNALFVAAVQLLRSSHAPQWQQAALV
                     PMFALFYVVLAPFVGALADSMPKGRVMLISNFIKVGGCLFMLFGTHPLMAYAVVGLGA
                     AAYSPAKYGILTELLPPSQLVKANGWIEGLTIGSIILGVLLGGQLVGPHVAPKLLAID
                     FPLINTGINNAPEAAISVLIFVYLLAAWFNTHIPLTGVLMRPM"
     gene            732..1856
                     /gene="alr"
                     /locus_tag="PLB25_01170"
     CDS             732..1856
                     /gene="alr"
                     /locus_tag="PLB25_01170"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HOE40235.1"
                     /translation="MPRPILATIHTAALQHNLARARLAAPDAKAWAVVKANAYGHGIE
                     QVFEGLRSADGFALLDLSEAQRVRNLGWRGPILLLEGVFEPRDLELCSRLDLWHTVHC
                     EEQINMLAAHKTNLGHRVFLKMNSGMNRLGFTPERYRAAWARLNALPQVDEISLITHF
                     SDADGARGVQQQLQVFECVTRDLPGERSLSNSAATLRHSDTGNIAAPELVISDWIRPG
                     ILLYGSAPDHPEHSSAQWDLLPSMTLATKIIATQAIDTGASVGYGSAFVADRPTLVGV
                     VAGGYADGYPRLCPTGTPVLVAGRRTRTLGRVSMDMIAVDLTPLADAGVPVGVGSAVT
                     LWGRADNGAVLPIDEVAQAAGTLGYELMCALAARVPVVVD"
     gene            1817..3076
                     /locus_tag="PLB25_01175"
     CDS             1817..3076
                     /locus_tag="PLB25_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40236.1"
                     /translation="MCAGRAGAGGGGLMQVFVPVPLSRVQLGRVLPVDVWTPDGRLLM
                     RHGQPLQSEAHRELLAAHGACMTETDALAWQMSLERSMRQMRADGVDMATIARAPMPA
                     EIADTDYQEGRVVEGGWLDLQEVLRGLLYQGADASNPLPRLQGIEQKAQALLRRDPDE
                     ALFVLFQALPDLRLGYCATHALLAGVVAALTADKLEQPEDSPALLLRSALVMNIGMAR
                     PQDSLARQLRPPNPAQRQIIAAHGPASTDILRSFGLHEDELLSLVQWHHQPQAAALQP
                     PQHGYLRMLNLADSLIAKMAPRSSRAAMLPLAAAKSLMQTTSPDTADLRPAMAAVLGF
                     YPPGSYVQLVNGETAVVVKRGRRANAPHVATIMNASGMPIAKYVYHDTSDSLAPRYAV
                     LAPVASATVKVSVSLEKVRRLRHQNGV"
     gene            complement(3030..5867)
                     /locus_tag="PLB25_01180"
     CDS             complement(3030..5867)
                     /locus_tag="PLB25_01180"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HOE40237.1"
                     /translation="MTPNDSLNNEPSSTNLANAGLSAVQAGQRLAADGPNALPTDQQR
                     GWLAIMLETLSDPMFALLLGAGVLYLVLGDLQEGLILFGLVLVVLALTLYQEGKTEHA
                     LASLRDLTSPRALVLRDGAPLRIAGRDVVQGDVLVLAEGDRIAADALLFEGTEVQVDE
                     SLLTGEPVPVDKLAGSSLGQQAGAGSTKSAASPGAPGQAVSPTTQDASSTLFSGTLLV
                     RGHGLARVTATGPRSEIGRIGTALESLTSEASPLRKQTAQLVRSLAILAVVVSLLLVL
                     VVGLLKGDWLAALLAGIALAMALLPQEFSVVLTVIPALGAWRLSKHKVLTRRIAAIET
                     LGATSVLCVDKTGTLTENRMTVTQLYAEQSAGINHPQGSVAGIAGDSLAIDYASSTGL
                     PETFHTLVEFSILASVSDPFDPMEKAFHRLGQHFLSDTEHLHRDWTLMQQYGLTPELR
                     AMSHVWKAVDGQDHVVAAKGAPEAIIDLCHMDSVAQSRVAGIVEAMAAKGLRVLGVAR
                     ARYQGMDWPAIEHDFEFEFVGLLGLADPLRPEIAEAVAQAHTAGIRVVMITGDYPATA
                     QAIAFEAGLQTRAGPAPTGLPDASQTSQPGRSPAQAVPAQAVPAQAAPAPAGSASAVP
                     GSNDWLLTGDAMDRLDDARLQQRMTSVCVCARIAPTQKLRIVQALKANGDVVAMTGDG
                     VNDAPALKAAHVGVAMGERGTDVAREAASIVLLDDNFAGLVHAVRLGRRIFDNLRKSM
                     SYILAVHVPIAGMALLPVLLGWPVVLFPLHIAFLELVIDPACSMVFENEPPEPDLMQR
                     PPRDVTTPLFGGKTLVWALLQGLGALALVLAATWWGLGHLSEGAARAFSFATLVAGNL
                     ALLLVNRARVGSLWQSLRVPNLTLWLVCAAALMLMGLALYVPWLAGLFKFELLPLPWL
                     GAALALGLLLVLGFELVKPRSDGASGALFPG"
     gene            6093..7502
                     /gene="radA"
                     /locus_tag="PLB25_01185"
     CDS             6093..7502
                     /gene="radA"
                     /locus_tag="PLB25_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HOE40238.1"
                     /translation="MVKEKTVYVCSDCGGTSPKWLGKCPHCNAWNTLIESVPESSVPA
                     KNRFASLAKTAGIAILGDIDAVDMARTPTGHDELDRVLGGGMVEGGVVLIGGDPGIGK
                     STLLLQALDSLQRSGQSTLYVTGEESGAQVALRARRLGLNGSQVRVLAEIQLEKILAA
                     LTATRPDIAVIDSIQTVYSDQLTSAPGSVAQVRECAAHLTRYAKASAGAESGAPGRAT
                     CIVLVGHVTKEGALAGPRVLEHMVDTVLYFEGDTHSSFRLVRAIKNRFGAVNEIGVFA
                     MTEKGLKGVSNPSAIFLSQHAEPVPGSCVMVTLEGTRPMLVEIQALVDSGGPSPRRLS
                     VGLDRDRLAMLLAVLHRHAGVACLDQDVFVNAVGGVRISEPAADLAVLLAITSSLRGK
                     PLPRGFFAFGEVGLAGEVRPAPRGQERLKEAAKLGFSMAVVPKANLPKNPRARDFEGL
                     TIHAVERVEQAMELVRELG"
     gene            complement(7765..8115)
                     /locus_tag="PLB25_01190"
     CDS             complement(7765..8115)
                     /locus_tag="PLB25_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOE40239.1"
                     /translation="MSADTDLWTVAVIIGLTLITVLTRTLFFISSKPWHLPHWAQRGL
                     QYAPIAALSAVIVPELVMTQGQFIQNWQDARLFAALAGAVWFFWHKGQGQAVLGTIVT
                     GMLVYLPLHLGLGW"
     gene            complement(8112..8846)
                     /locus_tag="PLB25_01195"
     CDS             complement(8112..8846)
                     /locus_tag="PLB25_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOE40240.1"
                     /translation="MFFRPELYRHPEFRSGALESLSAAPGLAAWGLMTGVAMVNSGLS
                     TFEALLMGVIVFAGSSQLAAIPLIAAAAPMWVVLATGFCVNLRFVVFSAHMRPYMMHL
                     PLWQRLLSGYLTTDLSYVLFTRRYPQHGGTPEQLLAQQAYLAGNSGVNWLAWVGCSVL
                     GVLLARLIPMAWGLGFAGILALLGILFSLASSHLRWVSAGVSGAAAVAAFALPFKLNI
                     LLAIAAAVAMCLMLEASREAAGPGPP"
     gene            complement(8852..9820)
                     /gene="fmt"
                     /locus_tag="PLB25_01200"
     CDS             complement(8852..9820)
                     /gene="fmt"
                     /locus_tag="PLB25_01200"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HOE40241.1"
                     /translation="MSALKLVFAGTPEFARVALAGLQSAGHDIALVLTKPDQPARRGL
                     KLQPSPVKQFALAHGMPVAQPRSLRLDGKYPDDARAVQALLQTGGFDAMVVAAYGLIL
                     PQWVLDLPTKGCFNIHASCLPRWRGAAPIHRAIEAGDASTGVTIMQMDAGLDTGDMLL
                     AQSLPILDTDTAATLHDKLAELGAALMLQTLQQVQQGNTQPVAQPPQGITYAHKIDKL
                     EAVIDWAQAAQTIVRRVRAFNPAPVANTRVAGETLKIWAAQASPEIPPAELKYGTIVA
                     VDHTGIAVEAINSIVILTELQRPGGKRLQAADFLRGFDLRVGQRFD"
     gene            complement(9820..10329)
                     /gene="def"
                     /locus_tag="PLB25_01205"
     CDS             complement(9820..10329)
                     /gene="def"
                     /locus_tag="PLB25_01205"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOE40242.1"
                     /translation="MALLPILCYPDPKLRTIAKPVTTVDARIKTLIANMLETMHEAKG
                     IGLAATQVDVHECLIVIDVAEGRDDPLVLINPKIIWRSPETHLNEEGCLSVPGIYDGV
                     QRHDAVKVEAQDGDGKLRLIEADGMLSVCIQHEMDHLLGKVFVEYLSPLKRNRIKTKL
                     LKAQREARA"
     gene            10460..11662
                     /locus_tag="PLB25_01210"
     CDS             10460..11662
                     /locus_tag="PLB25_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HOE40243.1"
                     /translation="MAKPSFNAIPLQVGLAAIIIGLQLATPAIAQNFSVTAQQRATAS
                     QVANSGVPLSQIKADAPPSYTVKSGDTLWAIAGMYLKSPWRWPELWGMNLDDIKNPHL
                     IYPGQTLFLVRKDGLARLSMSDSDLPTVRLSPRIRPEKLALSALPTLKSHLIEPYLSE
                     PVIVDEQGLAAAPRIVAAQEDRVLLTRGDRAYARSSGGEELTDDPSLKQKAYRVFRDA
                     TALKDPITGEVLGYEAQYVGKALLVQSEATQESTDRNGKVRSDLVPATIDIVDAKEEM
                     RVGDRLLPEPPLQFVSYVPHAPQNPVDARVVSIYGNAVANAAQNQVIVINQGERDGIE
                     PGHVLAILKDGTRLVDKTDPSRPELKLPDERNGLLMVFRTFEKLSYALVLEVTTGVLV
                     GDRVVNPL"
     gene            11678..12901
                     /gene="dprA"
                     /locus_tag="PLB25_01215"
     CDS             11678..12901
                     /gene="dprA"
                     /locus_tag="PLB25_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HOE40244.1"
                     /translation="MEVDELTAWLRLSLSSGVGNSSARKLLAAFGLPQAIFEQSALAL
                     EQTVSSAQVQALRSVPEGLAALLDTTLAWLQQDVPDGIRRQLTTLGDPGYPQALLNLD
                     DPPLMLYLLGAAHFKQNSPPALALQAQAATDVIANSISVDAARSLAVVGSRNPTPQGV
                     ANAQRFAQSLVEAGVTVVSGLALGIDGAAHEGALKATGSTQVGATLAVVGTGLDRVYP
                     KAHRDLAHRIAERGMLVSEYPLGTPPLTANFPRRNRLIAGLAQGTLVVEAALKSGSLI
                     TARLTNEQGKEVFAIPGSIHAAQSRGCHALIKQGAKLVESAQDIFDELKWLDGPSHDT
                     KLIAGDADTERSGGLFEHDIGLLTGLGFDPVGLDALQARTGLDAASLQAQLMSLELDG
                     LVARLPGGLFQRQPT"
     gene            13293..13814
                     /locus_tag="PLB25_01220"
     CDS             13293..13814
                     /locus_tag="PLB25_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HOE40245.1"
                     /translation="MFEVLAFVYQNYWDGDACPELPALQRKLGAVGFDSHEVVDALVW
                     LEDLKHAARGLHRPQAVQPGTVTASPHQLADARNPWQNRQPAMRLFTDAEQARLGATG
                     WRFVAFLASIGALPQDRLELVMERTMAASGDPISVEDLKLIVLMVFWSLGEEPDALVF
                     DELCDNRIDRLAN"
     gene            complement(13823..16198)
                     /locus_tag="PLB25_01225"
     CDS             complement(13823..16198)
                     /locus_tag="PLB25_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HOE40246.1"
                     /translation="MATTLEPRQAANLAAQARQRLVALATEAMASVLGLVQERLSSLM
                     NEAAPSREMQLRRDAWMAFQRFKQAWCDGTVQTWKAALLPETRLAPVRAGITMELLGT
                     EVVENKIIASRLALDLMEKSALEVNSLRKRLKFLAGEQELSARDIVHPEVLFLALVER
                     WETSGLPRESWQLTNAVVQRHFNEQLQKAYARCNADLIAQGVLPVIDFSPKLNPVAAG
                     PGASSTAPAADSGDASPTPAPAEPAPSLHGRRAEDRAMAGGIFNNLAGMQGMPGMPGM
                     QGYAGGSYADAARAGAVGERRFGRAQVLLEQVGRLLGGFFNPDSPQGSHIAPSVPLMT
                     ALARQPRLSAPNFEMVAGEPPTGQMVVINRVVRELREQSAELKGKAQTDNEKAIIELV
                     ALMFQAILQEDRIPTGVRVWFARLQMPVLRVALAEPDFFNKLNHPARQLIDHMGSCVM
                     GFDASGISSTALELEIKRVVQVIEQYPDTGDRVYKKVYEEFQAFLKQHLTEKPSTQKV
                     VGVAQQLEQKETLTIQYTIDLRDQLKDMPVRDEIREFLYKVWAEVLAVSAVRQGGQHK
                     QTLLLKKTATDLIWAASAKPNRADRARVIANLPELLQSLRTGMTLMGVNRSAQDAHIK
                     IISDTLADAFMSKTQAIAEDQIAALAERLANLEDYISDDGNENLPLDSNNIEELLGIE
                     ASELDVLSSGTSTASPAMLQWARELDLGAWFTLDHNHQIAQVQYVWRSPLGHLHLFAN
                     TLGHSYLIQSARLAAYLQEGLLSPQEDESLTMRATRDALGKIQANPERLLR"
     gene            complement(16213..>17013)
                     /gene="secF"
                     /locus_tag="PLB25_01230"
     CDS             complement(16213..>17013)
                     /gene="secF"
                     /locus_tag="PLB25_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HOE40247.1"
                     /translation="VTYAQPADLATVRSTVDKLGFADVQVQNFGTARDVLIRLPVQKG
                     VSSAQQSTQVVAALKAVDASASMRRTEFVGPQVGDELAADGLKALAFVVIGIMVYLAV
                     RFEWKFAVAAIIANLHDVIIILGFFAYFQWEFSLPVLAAVLAVLGYSVNESVVIFDRI
                     RENFRRYRKMTTIEIINNAITSTISRTIITHGSTQMMVLSMLLFGGATLHYFALALTI
                     GILFGIYSSVFVAAAIAMWLGIQREDLVKGQLAKKDLDPNDPNAGATV"
BASE COUNT         3342 a         5238 c         5240 g         3193 t
ORIGIN      
        1 catgggccgc atcagcacgc cagtcagcgg aatgtgggta ttgaaccagg cggccaacaa
       61 atagacaaaa atcagcaccg aaatggcggc ttcaggcgcg ttgttgatgc cggtgttgat
      121 cagcggaaaa tcaatggcca gcagtttggg agccacatgc ggcccgacga gttgcccgcc
      181 cagcagcacg cccaggatga tcgagccaat tgtcagcccc tcgatccagc cattggcctt
      241 gactagttgc gagggtggca gcagctcggt cagaatgcca tatttggcgg gcgaataggc
      301 cgcagcgccc aggccgacca ctgcataggc catcaacgga tgggtgccaa acagcatgaa
      361 caggcagccg ccgactttga tgaaattgct gatcagcatg acccggccct tgggcatgga
      421 gtctgccagc gcccctacaa acggagccag cacaacataa aacaacgcaa acattggcac
      481 cagagccgcc tgctgccact gcggcgcgtg gctgctgcga agcaattgca cggcggctac
      541 aaacaaggcg ttgtcggcca acgagctgaa aaactgcgct gccatgatgg tgaaaaaacc
      601 gcgtttcatg tgtgcccgaa aggcctcacc cagtgagctg ccgagataag tgatgtgtct
      661 tcaaatgtgg tgggggttat agcacggtcg ggcggtgtca aaattacccc catcgctttg
      721 acctgaaacc tatgccacgc ccgattcttg ccaccattca cactgctgca ctgcaacaca
      781 atctggcgcg agcgcgcctg gccgcgccag atgccaaagc ctgggcggtg gtcaaggcca
      841 acgcctatgg ccacggcatc gagcaggtat tcgagggcct gcgcagcgcc gacggatttg
      901 cgctgctgga cctgtccgaa gcacagcgcg tcagaaacct gggctggcgc ggacccatac
      961 tgttgctgga aggcgttttt gagccgcgtg acctggaact gtgttcgcgt ctggacctgt
     1021 ggcacacggt gcattgcgaa gagcagatca acatgctggc ggcacacaaa accaatctgg
     1081 ggcatcgggt gttcctgaaa atgaactcgg gcatgaaccg gcttggcttc acccccgagc
     1141 gttaccgtgc ggcttgggcg cgcctgaacg cgctgcccca ggtcgatgag atttcgctga
     1201 tcacacattt cagcgatgca gatggtgcgc gtggcgtgca gcagcaactg caggtgtttg
     1261 agtgtgtcac gcgtgatttg ccgggcgagc ggtccttgag caacagcgct gccaccttgc
     1321 gccacagcga tactggcaac atcgccgcgc cagagctggt gatcagcgac tggattcgcc
     1381 ctggcatctt gctgtacggc agtgcacccg accacccgga gcacagcagc gcccaatggg
     1441 atttgttgcc aagcatgacg cttgccacaa aaataatagc tactcaggca atagacacgg
     1501 gcgctagcgt tggatatggc tctgcttttg tggctgatcg gccgaccctg gtgggggttg
     1561 tcgctggcgg ctacgctgac ggctacccgc gcctttgccc gaccggcacg ccggtgctgg
     1621 tggcgggccg tcgcacccgc acgctcggac gcgtcagcat ggacatgatc gcagtcgacc
     1681 tgacacccct ggcagatgcc ggtgtgccgg tgggggtggg cagtgcggtc acgctgtggg
     1741 gccgcgccga caatggcgct gtgctgccga ttgacgaagt tgcccaggcg gcgggcacac
     1801 tgggctatga gctgatgtgt gcgctggccg cgcgggtgcc ggtggtggtg gactgatgca
     1861 ggtgtttgtt ccagtgccat taagccgggt gcagcttggc cgggtattgc cggtcgatgt
     1921 ctggaccccg gacgggcgct tgctgatgcg ccacgggcaa cccctgcagt ctgaagcgca
     1981 ccgcgaattg cttgccgcac acggggcctg catgaccgaa accgatgccc tggcctggca
     2041 aatgagcctg gagcgcagca tgcgccagat gcgtgccgac ggcgttgaca tggctaccat
     2101 tgcacgcgca ccgatgccag ccgagatcgc tgacaccgat tatcaggagg gccgggtggt
     2161 cgaaggcggc tggctggacc tgcaggaggt tttgcgtggc ctgctgtacc agggtgctga
     2221 tgccagcaac ccgctgccgc gcttgcaagg cattgagcaa aaggcccagg cactgctgcg
     2281 gcgcgacccg gacgaggcgc tgtttgtgct gtttcaggcg ctgcccgacc tgcgcctggg
     2341 ctattgcgcc acccatgccc tgctggccgg cgtggtggct gcgctgaccg ccgacaagct
     2401 tgagcagccc gaagacagcc cggcgctgct gctgcgctcg gctctggtga tgaatatcgg
     2461 catggcgcgg ccgcaggaca gcctggcgcg ccagctcagg ccgcccaatc cggcccagcg
     2521 ccagatcatt gccgcgcatg ggccggccag caccgacatc ctgcgcagtt ttggcctgca
     2581 cgaagatgag ctgttgagcc tggtgcagtg gcatcatcag ccgcaggctg cagcgctgca
     2641 gccgccgcag cacggctacc tgcgcatgct gaacctggcc gacagcctga tcgccaaaat
     2701 ggcaccgcgc agcagccgcg ccgccatgtt gccgctggcc gccgccaagt cgttgatgca
     2761 aaccacgtcc cccgacacgg ctgacttgcg cccggcgatg gctgcagtgc tgggttttta
     2821 cccgcccggc agctatgtgc aactggtcaa cggggagacg gcggtggtgg tcaagcgcgg
     2881 taggcgtgcc aacgcgccgc atgtggcgac catcatgaac gccagtggca tgccgattgc
     2941 caaatatgtg taccacgaca ccagtgacag tctggcaccc cggtatgcgg tgctggcccc
     3001 ggtggccagt gccaccgtga aggtcagcgt cagcctggaa aaagtgcgcc gcttgcgcca
     3061 tcagaacggg gtttgaccag ctcaaaaccc agcaccagca gcagtccaag cgccaaagcc
     3121 gcgccgagcc agggcagcgg caacagttcg aacttgaaca aaccggcgag ccatggcaca
     3181 tacagcgcca ggcccatcag catcaatgcc gccgcgcaca ccagccacaa ggtgaggttg
     3241 ggcacccgca gactttgcca gagcgagcca acccgcgccc ggttgaccag aagcagggcc
     3301 aggttgcctg ccaccagcgt ggcaaaggaa aaggcgcgcg ccgcaccttc gctcaaatgc
     3361 cccaggcccc accaggtggc ggccaacacc aacgccagcg ccccaagccc ttgcaacagc
     3421 gcccagacca gggtcttgcc accaaacagc ggtgtcgtca catcgcgggg cgggcgctgc
     3481 atcaggtcag gctcgggtgg ctcgttctcg aacaccattg agcacgccgg gtcaatcacc
     3541 agttccagaa aagcaatgtg cagcgggaac agcaccaccg gccagcccag caacaccggc
     3601 agcagcgcca tgccggcaat cggcacatgc accgccagaa tgtaggacat ggacttgcgc
     3661 aggttgtcaa aaatgcgtcg ccccaggcgc actgcgtgca ccagtccggc aaagttgtcg
     3721 tccagcagca cgatcgatgc cgcctcgcgc gccacatcgg tgccgcgctc acccatcgcc
     3781 accccgacat gcgcagcttt gagcgctggt gcgtcgttga caccgtcacc ggtcatggcc
     3841 accacgtcgc cattggcctt gagggcttgc acgatgcgca acttttgtgt cggggcaatg
     3901 cgggcgcaga cgcagacgct ggtcatgcgc tgctgcaatc gtgcatcgtc caggcggtcc
     3961 atcgcatcgc cagtcagcag ccagtcgttg ctgccgggca cggcgctggc agaacccgcc
     4021 ggggccggtg ctgcttgggc tggaaccgct tgggctggaa ccgcttgggc tggagagcgg
     4081 cctggctggc tggtctggct tgcatcaggc aggccagtcg gcgctggccc ggctcgggtc
     4141 tgcaaaccgg cttcaaacgc aatcgcctgt gcggtggcgg ggtagtcgcc agtgatcatc
     4201 accacccgga tgcctgccgt gtgggcctgc gcgaccgcct cggcaatctc ggggcgcagc
     4261 gggtcagcca ggcccagcag gccgacaaac tcgaactcga agtcatgctc gatggcgggc
     4321 cagtccatgc cctgatagcg cgcgcgcgcc acgcccagca cccgcagccc cttggcggcc
     4381 atggcttcaa cgatgccggc gacacggctc tgggcaactg agtccatgtg gcacaaatcg
     4441 atgatcgcct cgggcgcgcc tttggcagcc accacatggt cctggccatc gaccgctttc
     4501 cagacatgcg acatggcgcg cagttctggc gtcagaccgt actgttgcat cagcgtccag
     4561 tcgcggtgca gatgttctgt gtcactcaaa aaatgttgcc cgaggcggtg aaaggccttc
     4621 tccatcgggt caaacgggtc gctgacgctg gccagaatcg aaaactccac cagggtgtgg
     4681 aaggtctcgg gcaacccggt gctgctggcg tagtcaattg ccagactgtc gcctgcaatg
     4741 cctgcaaccg agccttgcgg gtgattgatg cctgccgact gttcggcata caactgtgtc
     4801 accgtcatgc ggttttcggt caacgtgccg gttttgtcca cacacagcac gctggttgca
     4861 cccagcgtct caatcgccgc aatgcgccgt gtcagcacct tgtgttttga cagtcgccag
     4921 gcccccaacg ccggaatgac cgtcagcacc acgctgaact cctgcggcaa cagggccatc
     4981 gccagcgcaa tgccggccag cagcgcagcc agccagtcgc ctttgagcaa accaaccacc
     5041 agcaccagca acaggctgac gacgactgcc aggatagcca gtgagcgcac cagttgtgca
     5101 gtttgtttgc gaagcggcga ggcttccgag gtcagcgact ccagcgccgt gccgatgcgg
     5161 ccgatttcac tgcgcgggcc ggtcgcggtg acgcgcgcca ggccgtgacc acgcaccagc
     5221 agggtgccgg aaaacagggt cgaagacgcg tcctgagttg tcgggctgac agcttgccca
     5281 ggcgcgcccg gactcgctgc tgactttgtt gacccggccc cggcttgctg acccaagctg
     5341 ctgccagcca gtttgtcaac cggcactggc tcaccggtca gcaacgattc gtcaacctgc
     5401 acctcagtgc cctcgaacag cagcgcatcg gcggcaatgc ggtcaccttc ggccagcacc
     5461 agcacatcgc cttgcaccac gtcgcgcccg gcaatgcgca acggtgcgcc gtcacgcagg
     5521 accagcgcac gcgggctggt caggtcacgc aaggaggcca gcgcgtgttc ggtcttgcct
     5581 tcctggtaca gggtcagggc cagcaccacc agcaccaggc caaacagaat caggccttcc
     5641 tgcaggtcgc cgagcaccag ataaagcacc ccggcaccca gcagcagcgc aaacatcggg
     5701 tcagacagcg tctcaagcat gatcgccagc cagccgcgct gctgatcggt cggcaaggca
     5761 ttggggccgt cggcggccag gcgttggcct gcctgcacgg cgctgagtcc ggcgttggcc
     5821 agattggtgc ttgaaggctc gttgttcagc gaatcgttag gtgtcatatt ggcctctagc
     5881 ccttgtgaat attgcgctaa cagctattat tacaatagca atcgacattt ccccaaagcc
     5941 gtggatcgct ggattttctg ccgcaaacgg acgacatcgc gcaggttgac cgggatgcga
     6001 ccgattattt ggcatggatc gcgtcaggga ccgtctgaca gcgcaccgac agtcaagctc
     6061 acatctgtgt ttttagccag tatcatgctg ccatggtcaa agaaaaaact gtttacgtct
     6121 gctccgactg tggcggcacc agccccaagt ggctgggcaa gtgcccgcat tgcaatgcct
     6181 ggaacacgct gattgaatcg gtgccagaaa gcagcgtgcc tgccaaaaac cgctttgcgt
     6241 cactggccaa aaccgccggt attgccatcc tgggcgacat tgacgcggtt gatatggcgc
     6301 gcacccccac cggccacgac gagcttgacc gggtgttggg cggcggcatg gtcgaaggtg
     6361 gtgtggtgct gatcggcggc gaccccggca ttggcaaatc gactttgctg ttacaagcgc
     6421 tcgattcgct gcagcgcagt ggccaaagca ccttgtacgt gacgggcgaa gaaagtggcg
     6481 cgcaagtggc actgcgcgcc cgtcgtctgg ggctcaatgg ctcgcaggtg cgggtgctgg
     6541 ccgaaatcca gcttgaaaaa atcctcgccg cgctgactgc cacgcggccc gacatcgccg
     6601 tgatcgactc gatccagacg gtctattcag accagctcac ctcggcccca gggtcggttg
     6661 cgcaggtgcg cgagtgtgct gcgcacctga cccgctacgc aaaagccagc gctggcgctg
     6721 agagtggtgc ccctgggcgg gcaacctgca tcgtgctggt cggccatgtc accaaagaag
     6781 gcgctttggc tggcccgcgc gtgctggagc acatggttga taccgtgctg tattttgaag
     6841 gcgacacgca ttcgagtttt cggctggtgc gcgccatcaa gaaccgcttt ggtgccgtca
     6901 acgaaatcgg cgtgtttgcc atgaccgaaa aaggccttaa gggggtcagc aacccgagcg
     6961 ccatcttcct gagccagcac gccgagccgg tgccgggcag ttgtgtcatg gtcaccctgg
     7021 agggcacgcg cccgatgctg gtcgagattc aggcactggt cgatagcggc gggccgtcgc
     7081 cccggcgctt gagtgtcggc ctggaccggg accgcctggc gatgctgctg gcggtgctgc
     7141 accgccacgc cggggtggcg tgtctggatc aggacgtgtt tgtcaatgcg gtcggcggtg
     7201 tgcgcatttc agaaccggcg gccgacctgg cggtgctgct ggcaattacc tcgtccttgc
     7261 gcggcaagcc gctgcccagg ggcttttttg cctttggtga ggttgggctg gccggtgagg
     7321 tgcgaccggc cccgcgcggc caggagcgcc tgaaggaagc cgccaagctg ggttttagca
     7381 tggccgtggt gcccaaagcc aacctgccga aaaaccccag ggcgcgggac tttgaaggcc
     7441 tgacgattca cgccgtggaa cgggttgagc aggcgatgga attggtgcgg gaactggggt
     7501 agcaatcgat caattcggcc ccaatgccag ttgacagctt gatcacttca acacgtgaat
     7561 gactcagatc attgctttcg atggggctca ccagttgggc gactgcacta ctcgcccggc
     7621 tgcgccattg gcagcggcac tgggggcgtt gctgctggct catccgccag gacgacatgc
     7681 gggccaaacc cgctgtgtcg ttgcgctcag gcacgccgcg tgcagtcaat cggtcgcgtc
     7741 caactgagca atccgggttg caccttacca gcccagtccc aggtgcaatg gcagatagac
     7801 cagcatgccg gtgacgatgg tccccaacac cgcctggcct tgtcccttgt gccaaaaaaa
     7861 ccatacagcc ccggccagcg cggcgaacaa gcgggcatct tgccagttct gaatgaactg
     7921 accctgtgtc atgaccagtt cgggcacgat caccgccgac agggccgcaa tcggcgcgta
     7981 ttgcaggccg cgctgagccc agtgcggtag atgccagggc ttgctggaga tgaaaaagag
     8041 ggtgcgcgtc agcaccgtga tcagcgtcag gccaatgatc actgccaccg tccagaggtc
     8101 ggtatcagca ctcatggcgg gcctggccct gcggcttcgc gcgacgcttc aagcatcaga
     8161 cacatggcca ccgctgccgc aatcgccagc agaatgttga gtttgaaagg cagcgcaaaa
     8221 gccgccaccg ctgcagcgcc ggacacgccg gcgctgaccc aacgcagatg gcttgaggca
     8281 agcgaaaaca aaatgcccag caaggccagg atgccggcaa aacccaagcc ccaggccatt
     8341 ggaatcaggc gcgccagcag caccccaagc acgctgcagc cgacccaggc cagccagttg
     8401 accccgctgt tgcccgccag ataggcctgc tgagccagca attgttcagg cgtgcctcca
     8461 tgctgcgggt agcgccgggt gaacagtaca tagctcaaat cggtggtcag gtagccgctg
     8521 agcaggcgct gccacaaggg caggtgcatc atgtaaggac gcatgtgggc gctgaacacg
     8581 acaaaacgca agttcacgca aaagccggta gccagcacca cccacatcgg cgccgctgcg
     8641 gcaatcaggg gaatcgccgc caattgcgag ctgccagcaa agacaatgac acccatcagc
     8701 agcgcctcaa aggtgctcag gcccgaattc accatcgcca cgccagtcat cagcccccag
     8761 gccgctagcc caggcgcagc actcaatgac tctagcgcgc cgctgcgaaa ctcggggtga
     8821 cggtagagtt caggtcggaa aaacaccagg ctcagtcaaa acgctgacca acccgcaggt
     8881 caaaaccacg cagaaagtca gcggcctgca gccgtttgcc gccggggcgc tgcagttcag
     8941 tcaggattac tatagaatta atagcttcca ccgcaatacc agtatggtct acagccacaa
     9001 ttgttccata tttcaattca gcaggcggga tttcggggct ggcctgggcg gcccagattt
     9061 tcagcgtctc accggcgaca cgggtgttgg caaccggtgc cgggttaaag gcccgcacac
     9121 gccggacaat ggtctgtgcc gcctgggccc agtcaatcac cgcctcaagc ttgtcgatct
     9181 tgtgggcgta tgtgatgccc tgcggcggct gcgcgactgg ctgggtgttg ccttgctgca
     9241 cctgttgcag ggtttgcagc atcagagcgg caccgagttc ggccagtttg tcatgcaggg
     9301 tggcggctgt gtcggtgtca agaatcggca gggactgcgc cagcagcatg tcaccggtat
     9361 cgagcccggc atccatttgc atgatggtga caccggtgct ggcgtcgcca gcttcaatgg
     9421 cgcggtggat cggtgccgca ccgcgccagc gtggcaggca agaggcgtgg atgttgaaac
     9481 agcctttggt cggcaggtcc agcacccatt gcggcaggat cagtccataa gcggccacca
     9541 ccatggcatc gaagccacca gtctgcagca gcgcctgcac cgcccgcgcg tcgtccgggt
     9601 atttgccgtc cagccgcagg ctgcgcggtt gcgccaccgg catgccgtgc gccagggcaa
     9661 attgtttcac cggcgagggc tgcagcttca ggccgcgccg ggctggctgg tccggcttgg
     9721 tcagcaccag cgcaatgtca tggcccgccg actgcaaacc ggccagcgca acccgggcaa
     9781 actcgggtgt accggcaaac accagcttca aggccgacat caagcgcgcg cctcgcgctg
     9841 ggcctttagc agcttggtct tgatgcggtt gcgcttgagc ggggacaggt attcgacaaa
     9901 caccttgccc agcaagtggt ccatttcatg ctggatgcac accgacaaca tgccatcggc
     9961 ctcgatcagg cgcagttttc cgtcaccatc ctgcgcctcg accttgaccg catcatggcg
    10021 ttgcacgccg tcgtaaatac cgggaaccga taggcagcct tcttcgttca gatgggtctc
    10081 agggctgcgc cagatgattt ttgggttgat cagcaccagc ggatcgtcac gcccctcggc
    10141 cacatcaatg acgatcaggc attcatgcac atcgacctgg gtggcggcca gaccgatgcc
    10201 tttggcctca tgcatggtct ctagcatgtt ggcaatcagg gttttgatac gtgcgtcaac
    10261 cgtcgtcact ggcttggcga tggtgcgcaa tttgggatcg ggatagcaga gaatcgggag
    10321 taaagccatg gtgtctcatc gaaggtgtcg gtattttcgc tatttttccg tccttttgac
    10381 ggatcgtcag gcgacaatcg ttgttcaatc aagggcttgg gcgcaaaatt tcatgcgatt
    10441 taacagggta ccaaccacca tggcaaaacc ttcattcaat gcaatcccac tccaggtggg
    10501 tctggccgcg atcatcattg gcctgcaact ggccaccccg gccatcgcac aaaacttctc
    10561 cgtcacggcg cagcagcgtg ccaccgccag ccaggtggcc aacagtggtg tgccgttgag
    10621 tcagatcaag gctgatgcgc caccgagcta taccgtgaag tcaggtgaca cgctgtgggc
    10681 gattgccggc atgtacctta aatcgccctg gcgctggcct gaactgtggg gcatgaacct
    10741 tgatgacatc aaaaacccgc acctgatcta ccctgggcaa acgctcttcc tggttcgcaa
    10801 ggatggtctg gcccgcctca gcatgagcga cagcgatttg ccgacagtgc gactgtcgcc
    10861 acgcatcagg cctgaaaagc tggcactcag cgctttgcca acactcaaga gtcacctgat
    10921 cgagccctat ttgtcagagc cggtgattgt cgatgaacag ggtttggcgg ctgcgccccg
    10981 catcgtggca gcgcaggagg accgggtgct gctgacccgt ggtgaccggg cttacgcccg
    11041 cagcagcggc ggcgaagagc tcaccgatga ccccagcctg aaacagaagg cctaccgcgt
    11101 ttttcgagat gccaccgcac tgaaagaccc gattaccggt gaggtgctgg gttacgaggc
    11161 ccagtacgtc ggcaaggcct tgctggtgca aagcgaggcc acccaggaaa gcaccgaccg
    11221 caatggcaag gtgcgctccg acttggtgcc agccaccatc gatatcgtcg acgccaaaga
    11281 agaaatgcgg gtgggtgacc ggctgctgcc cgagccaccg ctgcagtttg tcagctatgt
    11341 gccgcatgca ccgcaaaacc cggtggatgc ccgcgtcgtc tcgatctatg gcaacgccgt
    11401 ggccaatgcg gcgcaaaatc aggtgattgt gatcaaccag ggtgaacgcg atggcatcga
    11461 acctggccat gtgctggcca tcctgaaaga cggtacgcgt ctggttgaca agaccgatcc
    11521 gagccgcccc gagctcaaac tgcctgacga acgcaacggc ttgctgatgg tgtttcgtac
    11581 ctttgagaag ctgtcctatg cgctggtatt ggaggtcacc accggcgttt tggtgggcga
    11641 ccgggtggtc aatccgcttt aacgaccctg ctgcgccgtg gaggttgacg aactcactgc
    11701 ctggctgcgt ttgtcgctta gcagcggggt cggcaacagc agcgcccgca agctgctggc
    11761 agctttcggt ttgccgcagg ccatttttga gcagtctgcg ctggcacttg agcaaaccgt
    11821 cagcagtgca caggtgcagg cgctgcgcag cgtgcccgag ggcctggcgg cgctgctcga
    11881 taccacactt gcctggctgc agcaggatgt gcccgatggc atccgtcggc aactgaccac
    11941 gcttggcgat ccgggctacc cgcaggcctt gttgaacctg gatgacccac cactgatgct
    12001 gtacctgctg ggtgctgcac atttcaagca aaatagcccg ccagcccttg cgctacaagc
    12061 acaagcagct actgatgtaa tagcgaattc gatctctgtt gatgctgcca gaagtctggc
    12121 tgtggtgggt agccgcaacc ccactccgca gggcgtggcc aatgcccagc ggtttgccca
    12181 atcgttggtc gaagcggggg tgacggtggt cagtggtctg gcgctgggca ttgatggtgc
    12241 ggcgcatgag ggtgcactca aggcgaccgg gtccacccag gttggcgcaa cgctggcggt
    12301 ggtgggcacc gggctggacc gggtctatcc caaggcgcac cgcgatttgg cccaccgtat
    12361 tgccgagcgc ggcatgctgg tcagtgagta cccgctgggc acaccgccat tgacggccaa
    12421 tttcccccgg cgcaaccgcc tgatcgccgg cttggcgcaa ggcacgctgg tggtggaggc
    12481 agcactgaaa tcgggctcgc tgatcaccgc gcgactgacc aacgagcagg gcaaggaagt
    12541 gtttgccatc cccggctcga tccatgcagc ccagtcgcgt ggctgccatg cgctgatcaa
    12601 gcaaggggcc aagctggtcg aatcggcaca ggacatcttt gatgaactga agtggcttga
    12661 cggcccttcg catgatacaa agttgatagc tggtgacgca gacacagaaa ggtctggagg
    12721 cttatttgag catgatattg gactgttgac cggactcggt ttcgatccgg ttgggctcga
    12781 tgccctgcag gcgcgcaccg gcctcgacgc agccagcttg caggcgcagt tgatgagtct
    12841 ggaactcgat ggcttggtgg cgcgcctgcc cggtggcttg tttcagcgac aaccgacctg
    12901 aaccgccaag agccggtcgg acttgccgat cacgcagcga gaatcgaccc cagcgagggg
    12961 tgccggattg gtcggccgat agtccggttg tgggccagtg aaccaacgca aaatcggccg
    13021 ctgcggccag ttctcagccg atgcgccaaa aggatgtggt gagaaatgac tttcccaatc
    13081 tgcacctgcg gatttgggcg cacagcagcg ttgcaagacg tttgtttagc cagttgaacg
    13141 ccttgcgcct ggccttgcat cccaacgcct tggggcaaga acctgaattg ttcccgacca
    13201 catcccaagt ccgaccggcg tcctggattg agtcccgttt tccgggcttg tttggaggtt
    13261 tgaggcattc gccgggcggt atattaacgg ccatgtttga agtccttgcc tttgtttatc
    13321 aaaactactg ggacggcgat gcctgccctg aactgccagc attgcagcgc aaactcggtg
    13381 ccgtcggctt tgacagtcac gaggtggtcg atgccctggt ttggctggaa gatctgaagc
    13441 acgccgcgcg cggcttgcat cggccacagg ctgttcagcc cggcaccgtg accgctagcc
    13501 cgcaccaact tgccgatgcg cgcaatccct ggcaaaaccg acagcctgcc atgcggcttt
    13561 ttactgacgc agagcaagcc cggctgggtg ccacaggctg gcgctttgtc gcctttctgg
    13621 cctccattgg tgccttgccg caagaccggc tggaacttgt gatggagcgc accatggcag
    13681 cgtcgggtga cccgatcagc gtggaagacc tgaaactgat tgtgctgatg gtcttttgga
    13741 gcctgggcga ggaacccgat gccctggttt ttgacgaact gtgtgacaac cggattgacc
    13801 ggttggcaaa ttgagacggc gctcagcgca gcagccgctc cggattggcc tggattttgc
    13861 ccagtgcatc gcgggtggca cgcatcgtca gcgactcgtc ttcctgcggt gacagcaagc
    13921 cttcctgcag gtaagcggcc agccgggctg actggatcag gtaactgtgc cccagggtgt
    13981 tggcaaacag gtgcaaatga ccaagcggac tgcgccagac gtattgcacc tgggcaatct
    14041 ggtggttgtg gtccagcgtg aaccaggcac ccagatccag ttcacgggcc cactgcagca
    14101 tggctgggct ggccgtgctg gtgccactgg aaaggacatc gagttcagag gcctcgatgc
    14161 ccagcaattc ttcaatgttg ttggagtcca gtggcagatt ctcgttgcca tcgtcgctga
    14221 tgtagtcttc caggttggcg agacgctcgg ccagtgctgc aatctgatct tcggcaatcg
    14281 cctgcgtctt cgacatgaag gcatcggcaa gcgtgtcgct gatgatcttg atgtgggcat
    14341 cctgggccga ccggttgact cccatcaggg tcatgccggt gcgcagcgat tgcagcagtt
    14401 ccggcaagtt ggcaatcacg cgggcgcgat cagcgcggtt cggcttggca ctggcagccc
    14461 agatcaggtc ggtcgctgtt tttttcagca gcagggtttg tttgtgctga ccaccctgtc
    14521 gaactgcgga aaccgccagc acttcggccc agaccttgta caaaaactca cggatctcgt
    14581 cgcgcaccgg catgtccttg agttggtcac gcagatcgat ggtgtattga atggtgaggg
    14641 tttctttttg ttcgagttgc tgcgctacac ccaccacttt ttgggtcgat ggtttttcgg
    14701 tcaggtgctg tttcaaaaac gcctgaaatt cctcgtaaac ctttttatac acccgatcac
    14761 cggtatccgg gtactgctcg atcacctgaa ccacccgttt gatctcgagc tcaagtgctg
    14821 tgctgctgat accgctggca tcaaaaccca tgacacacga gcccatgtga tcaatgagct
    14881 gcctggccgg gtggttgagc ttgttgaaaa agtccggctc ggccagtgcc actcgcagta
    14941 ccggcatttg caggcgggca aaccagaccc gcaccccggt gggaatgcgg tcctcctgca
    15001 atatggcctg aaacatcagc gcgaccagtt caatgatggc cttttcgttg tcagtctggg
    15061 ccttgccctt gagctcggca gactgctcgc gcagttccct gaccacccgg tttatcacca
    15121 ccatttgccc ggtcggcggc tcaccggcta ccatttcaaa gtttggcgca ctcaatctgg
    15181 gctgccgtgc cagtgccgtc atcaacggca cagaaggtgc tatgtgggaa ccctgcggtg
    15241 aatcaggatt aaaaaacccg cctagcaagc gtccgacctg ctccagcaaa acttgggcac
    15301 gtccgaagcg acgctcgccg acagccccgg ctctggcggc atcggcgtag ctgccgccag
    15361 cataaccctg catgcctggc attccaggca ttccttgcat gcccgccaag ttgttgaaga
    15421 tgccgccagc catggctcgg tcttcggcgc gccgcccatg caatgacggc gctggctcgg
    15481 ccggtgccgg agtcggtgaa gcgtcgcccg agtccgctgc cggagcagtt gaagaggcac
    15541 ccgggcccgc tgccaccggg ttaagtttgg ggctgaaatc gatcaccggc agcacccctt
    15601 gagcgatcag gtcagcgttg caccgggcat aggccttttg caattgctca ttgaagtgac
    15661 gctgtaccac ggcgtttgtc agttgccacg attcacgcgg caagccgctg gtctcccagc
    15721 gttcaaccag cgccagaaac aacacctcgg gatggacgat gtcgcgggca ctcagctctt
    15781 gttcgccagc caggaacttc agccgcttgc gcaaactgtt gacttccagc gccgacttct
    15841 ccatcaggtc aagtgccagt cgcgaggcaa tgatcttgtt ttcgaccacc tcggtgccga
    15901 gcaactccat cgtgatgcca gcccgtaccg gtgccagacg agtttctggc aacaatgccg
    15961 ccttccaagt ctgcactgtg ccatcacacc aggcttgctt gaagcgctgg aaagccatcc
    16021 aggcatctcg gcgaagttgc atttcacgcg acggggcggc ctcgttcatc aggctgctca
    16081 ggcgctcttg taccagcccc agcactgagg ccatggcctc agtggcgagc gccaccagtc
    16141 gctggcgtgc ttgagctgcc aagttggccg cttgtctggg ctcaagagtg gttgccatgg
    16201 tcgagtcgcc gactaaaccg ttgccccggc gttcgggtcg ttcggatcaa ggtctttttt
    16261 ggccaattgt cccttgacca ggtcttcacg ctgaatgccc aaccacatgg cgatcgcggc
    16321 ggcgacaaac accgaagagt agatgccgaa aaggatgcca atggtcaacg ccagggcaaa
    16381 gtaatgcaac gtggcgccac caaacagcaa catcgagagc accatcatct gggtcgaacc
    16441 gtgggtgatg atggtgcggc tgatggttga ggtgatcgcg ttgttgatga tctcgatggt
    16501 cgtcatcttg cggtagcggc gaaagttctc gcgaatacgg tcaaatataa ccactgactc
    16561 gttgaccgaa tagcccagca ccgccagcac cgcagcgagc accggcaggg aaaactccca
    16621 ttgaaagtag gcaaaaaagc ccagaatgat gatcacgtca tgcaggttgg caatgatggc
    16681 ggcgacagca aacttccatt caaaacgcac cgcgagatag accatgatgc caatcaccac
    16741 aaaggccagc gccttgagcc catcggcagc cagttcatcg cccacctgcg ggccgacgaa
    16801 ctcggtgcgg cgcatgctgg cgctggcatc caccgctttc agggccgcca cgacctgggt
    16861 gctttgctgg gctgaactaa cgcctttttg tactggcagg cgaatcagca cgtcacgcgc
    16921 ggtaccaaag ttttgcactt gcacgtcagc aaagccaagt ttgtccacag tgctgcgcac
    16981 cgtcgccaga tcggcgggct gcgcgtaagt gac
//
LOCUS       DAOSLL010000015        16898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_3589_length_16898_cov_4.663837, whole genome shotgun sequence.
ACCESSION   DAOSLL010000015 DAOSLL010000000
VERSION     DAOSLL010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 16898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16898
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3589_length_16898_cov_4.663837"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="PLB25_01235"
     CDS             <1..347
                     /locus_tag="PLB25_01235"
                     /inference="COORDINATES: protein motif:HMM:NF023352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3443 family protein"
                     /protein_id="HOE40248.1"
                     /translation="FIDTGSNGLYFDTSTLTACTLSSQMYGFYCPSSTTELSATLSGK
                     NAVSANVSFSVSNAQTLVIGTDLTALPSLAGQMGSRTVFDWGLPFFYGRSVFFGFDQK
                     SSPLGIGPLIAF"
     gene            772..2040
                     /locus_tag="PLB25_01240"
     CDS             772..2040
                     /locus_tag="PLB25_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40249.1"
                     /translation="MFAPKAVVLFGFSMSVAMAATADTFPTGNVFDLTVGVNSVYQPA
                     IDAITGGIVTNPVGFTSVSDMFDGAKLSSLMKLNSSYTDSSPSVIRMGYRGLPALIST
                     GTTPTIKFEIPAINLVQVFDTKPTRDGNLDDLNEYLKTSGNSLLDQMQKLLVKVSPVD
                     PVAGNPNSLQSQMMMADFDRSFTQFASNIKEATGESNNLFGIGLSFGSFTQHGVTNNA
                     MTIPLSYVFRSDIDPARQLTLYAPVTVSSVEGAKSYAVNFGASYRYPITDDWALTPGI
                     GYGITGSSDLGAGAAMLALSVTSQYVIHRDGYDIGIGNALGTYRSQKFSAGDYSSDPG
                     ISNTVFRNGVLLSFPAMVYGKKMAYEFSFINTRFYGSDLYSENYNEIGVTLGTNRSAN
                     SSRSYLRAGFTYLQGQNDIRGYRLNVGYWF"
     gene            2067..4412
                     /locus_tag="PLB25_01245"
     CDS             2067..4412
                     /locus_tag="PLB25_01245"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOE40250.1"
                     /translation="MVTQGGLIRNLCRVFGLAICCWFGLAAPASALTGNEQVSLAGTG
                     KFEALVSGMELMLDKQPMKSSDWYSLCYGYSRVKHYGKILDCLDSLDKSLAKGDTRTR
                     LFGLDDATAASHLMRAEALIELAEYDPALVQVQKALDWYQREKSDDKDIFINALAAKS
                     LATLALGRRAEAERLSAELEGFSLGMTGGDYVGAKSLAMARVNMALGNWQKVLDALAQ
                     DKIFGLRSFLDNLASGAFLLGINNWVWIELPRAFMQTKALLELGQLDAARSGYDKLLA
                     VPQVKANGEIYWMALYDRGRIAERQGQLEEAERFFQEAAEVIERQRRSINSEANKIGF
                     VGDKQAVYASMVANQFRQGRFGQSFESMERGKSRALVDLLASNQSQRLISPKSANAAK
                     VTQVLDRIRNADLDSRRQDESRIRSVSQAGGSNELQTLASALPSSLASLVSVSRLDLK
                     EAQSLVKPDEALIEYFAAGDELFATLVQSNSVHGVRINASQVESVVRSLRQNMQEGSP
                     QALGQLQALHKLLIEPLLPNIRVKHLSIVPHGVLHYLPFGALNDGKQDLIDRFTLRIL
                     PSLAVLKYLRPSQTKGIERLLVLGNPDLGRPDLDLPSAQNEAEKLAAKFKAEHLLLRK
                     KATRTAFQALAPGAAFIHVASHGQFEAGKPLQSGLILAADKPGGGINAGRLTVSDLYE
                     LALDAELVTLSACETGLGAVASGDDVVGLTRGFLYAGVSTVIASLWQVDDEATTYLML
                     QMYEHLQRGGYPDALRKAQIETRAKYPHPMFWAAFYLTGLN"
     gene            complement(4430..4864)
                     /locus_tag="PLB25_01250"
     CDS             complement(4430..4864)
                     /locus_tag="PLB25_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40251.1"
                     /translation="MLDLPIHRRANGQKAGANWAAAVGSQPLRHQGDRVLTMIVAKSI
                     SNSFVSRHPAADIANAMQGEITTTWMIWKGNKSPDGRAARYFGQHNGSTPGDAAEALT
                     VEIITTPKPKNKRALELIAQTKTWSEHQTRIAGFSLGNAGLF"
     gene            complement(4978..6033)
                     /gene="hypE"
                     /locus_tag="PLB25_01255"
     CDS             complement(4978..6033)
                     /gene="hypE"
                     /locus_tag="PLB25_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HOE40252.1"
                     /translation="MKVKKNYLRPIDFKHGRVDMTHGAGGRAAAQLIDELFAAAFDNE
                     YLRQGHDGALLPQPKGRLVMATDGHVISPLFFPGGDIGCLSVHGTVNDVAMSGAWPLY
                     LAASFIIEEGYPLADLKRIVESMASAARQAGVPIVTGDTKVVEKGKGDGVFISTTGVG
                     VVPDGLLIDGRQARAGDAIVVSGTLGEHGVAVMSQRESLTFETAIKSDTAALHTLVQA
                     MLQALPDPARLHVLRDPTRGGLATTLNEIARQSQVGMSLIEGDIPVLPEVAAACELLG
                     LDPLYVANEGKLIAICSPEAADTLLAAMRAHPLGHGAARIGTVTADDNCFVQMATRFG
                     GRRVVDWLSGEQLPRIC"
     gene            complement(6030..7169)
                     /gene="hypD"
                     /locus_tag="PLB25_01260"
     CDS             complement(6030..7169)
                     /gene="hypD"
                     /locus_tag="PLB25_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HOE40253.1"
                     /translation="MKYIDEFRDGDAARLIASKIAAEALPDQAYNFMEFCGGHTHAIS
                     RYGISGMLPANVRMIHGPGCPVCVLPIGRIDLAISLALDRGVMLCTYGDTMRVPASDS
                     LSLIKAKARGGDIRMVYSAADALALAEQHPHREVVFFAIGFETTTPPTALVLREAKAR
                     QLTNFSVLCCHVLTPAAITHLLESAQARELGAIPIDGFIGPAHVSIIIGSQPYVHFAR
                     TYHKPVVITGFEPLDVMQGILMLVRQMNSGRAEVENEFSRVVTATGNLAAQKVMAQVF
                     ELRESFEWRGLGEVPASALKIRPEFAAWDAEVKFDLHYASVPDHKQCECGTILRGIKR
                     PADCKLFGTVCTPESPMGSCMVSNEGACAAHYSYGRFQDIAVVAA"
     gene            complement(7237..7464)
                     /locus_tag="PLB25_01265"
     CDS             complement(7237..7464)
                     /locus_tag="PLB25_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOE40254.1"
                     /translation="MCLALPARVVQLLDGQRAVVDLGGVRKEVSIDLVDDAQIDDYVI
                     IHVGYAIGKIDPDEAARTLALFAELAQMEAA"
     gene            complement(7455..8696)
                     /locus_tag="PLB25_01270"
     CDS             complement(7455..8696)
                     /locus_tag="PLB25_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOE40255.1"
                     /translation="MALILAFGAFLKNTTCLFDSDAPHTVRWSVVHGDLSDPAACVSL
                     RDSVAKLLAQAAGRIDAVAHDLHPDFFSTHLALQTADELRVPAFAVQHHHAHIAALMA
                     EHGLARPVLGLALDGVGLGSDTLAWGGELLLVNLAGFERLAHLLPLTLPGGDVAAREP
                     WRMAAAALHACGRADLIAPRFGPVVGQQAAKTIAAMLRRSIHCPQTTAAGRWFDAAAG
                     LLGLSVRQTEEAQAAIALEQAAKRWLQDQALEVCADDWCVAQGGQISLLPLFNTWLDY
                     SPEIVPTSKPASAGQPAHLPNQWCSASAGLNLADPASVGEAAARFHLTLSTALAQQTM
                     RAAKQHSIADIALGGGCFFNQILKTHLTMQLENAGLRVWHPQTISCGDAGLALGQAWV
                     AAQQLHADHAATPQPESNSCV"
     gene            complement(9045..10193)
                     /gene="hypB"
                     /locus_tag="PLB25_01275"
     CDS             complement(9045..10193)
                     /gene="hypB"
                     /locus_tag="PLB25_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HOE40256.1"
                     /translation="MCVVCGCGNTSSGDQSAPGVNVQPGTGDLHFGAGAARVSVPGMS
                     QQRAIKIEADVLGANNLIAAQNRAHFASHGVTAYNLVSSPGSGKTTLLCATIEALRQH
                     RPDLAVAVIEGDQQTSFDADRIRATGAPAIQVNTGKGCHLDAPMVAEAFSRLDLHASG
                     HHHDGRGHGAEEQVNTHPHDHPHDHEYAEQAHSHGHEHAEVAHSHDHSHDHPHSHQHN
                     RDAGHDHDHGHAHGHGHTHSHDHDHADDRATGNQALLFIENVGNLVCPAMWDLGEAAK
                     VAILSVTEGDDKPLKYPDMFAAAKLMVLNKIDLLPHVSFDVARCKEFARRVNPGIEIL
                     ELSATTGVGMQTWLDWLLTDPAAGNGNPVAARIAELEAELVRLRALPA"
     gene            10583..11149
                     /locus_tag="PLB25_01280"
     CDS             10583..11149
                     /locus_tag="PLB25_01280"
                     /inference="COORDINATES: protein motif:HMM:NF016682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation C-terminal
                     domain-containing protein"
                     /protein_id="HOE40257.1"
                     /translation="MHSLIDEAGMARTAQTSGSALARQDGGAQAGTTSATTSHMGKQH
                     AIGQNHPIIWLHPEAPVLSVDAQPGTVANAVFAEVKTSVERLVDQGETRSVDLRFMQS
                     MPAERAALESLLGRGEVLAEVHAAGRSKVQETAIPCVWLIRHFNSEDELVGELIEVTD
                     VPEILVSDRQAVTHCLTQLNAKAPTGSN"
     gene            11188..11304
                     /locus_tag="PLB25_01285"
     CDS             11188..11304
                     /locus_tag="PLB25_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HOE40258.1"
                     /translation="MKKPSTVFDSLQQQGVSRRSFIKFCTVTASRWGLPEPV"
     gene            11301..12221
                     /locus_tag="PLB25_01290"
     CDS             11301..12221
                     /locus_tag="PLB25_01290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HOE40259.1"
                     /translation="MKAFTQALGAAQRPSVIWMSFQHCTGCSESLMRSGSPLSDRLTV
                     ENLILNFISLDYHETLQVAAGAAAEDARMAAMKANFGKYILIVDGSVPTAENEWWLAV
                     NGHSGLSLLHEAVAGAGLVLALGACATFGGIPAAFPNPSKAMGVSDLMDKGMIDKKPL
                     INISGCPPIPEVITGTIAYYLVNQAMPPLDALGRPLVFFGKTVHDCCPRLDHFNAGEF
                     ATSFDDDGARKGHCLLMLGCKGPDTFNACTTTKWNQGTSSPTQAGHGCIGCSEPNFWD
                     KPGGVYGFSSGFKTGDGTCAAPVLPSLPLI"
     gene            12267..14024
                     /locus_tag="PLB25_01295"
     CDS             12267..14024
                     /locus_tag="PLB25_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOE40260.1"
                     /translation="MSTIVIDPVTRIEGHLRIEAETDKSGVVTSALSSGTMMRGIEII
                     LKNRDPREAWAFTQRICGVCTVVHGMTSVRAVEDALGYTIPTNARHIRNLLMGSQLVQ
                     DQVTHFYQLTSMDWINVVSASQANPTTTAELQKCHSKWPNNTSAYFESVRQKLANFIA
                     SGQLGLFSNGYWDNPGYKLPPEMNLMLFAHWLEALEWQRKVLRMQAIFGGRNPHPNFT
                     IGGVACSFHMAPNAAGILVPAPGSTGVDQAGLDLVQSCITDMRNFVVQVLRPDVLALA
                     SYYKDWFTRGEGVGNFLTYGEYPQDDNADLAKNLFVPRGAILDRNLSNIQSVDLRKTD
                     EVQELIPSSWLGYTKGKSIGLHPFQGETTPDYTGPKAPYDKLDASKPYSWMKTPHWKG
                     HHMEVGPLARVLVMYGKRTASVRDHVDSALKDLGLSFDAMYSTMGRILAQSIDTHVVA
                     DQLQVWFNQLTANVAGGDFKTHNAAKFNPSTWPASCKGVGFTEAPRGALGHWVTIDAA
                     KISNYQCIVPTTWNAAPRDTGGKGGPYEMALLKHTLAVSSQPLELLRTIHSFNPCMAC
                     AVHIMDPRQDETLKVTVCA"
     gene            14054..15640
                     /locus_tag="PLB25_01300"
     CDS             14054..15640
                     /locus_tag="PLB25_01300"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HOE40261.1"
                     /translation="MNKMSASSRFFLAIWFAVLCAFTGSALALPSFGTQIDNYCALQP
                     TLAATPQQSRPYQASGSSCASCHTSNSPKKTDLNALGTASKTCTGSTCGASVKPFCIA
                     TAPSNASITAPAAGSSVALGQSMTFTAGNATNPDGFPLTYKWTFSSGQASVTGQSVAV
                     PMSVAGPVTATLQVLNSVSMLASGTAPTRAVTVTGAPTGNQAPSAAIGSPAANTTTTP
                     GGTVNFQGSGTDPDNNVPLSYSWTFPGGSPSSSVVQNPGAVTYATAGTFSASLTVRDS
                     LGLASVAVTRTVTVSTPGANQAPTAIISSPAADTAVIPGASVNFQGSGTDPDNNTPLT
                     YKWTFSGGSPATSTAQNPGTVTYSAAGTYTASLTVTDSKGLASTATSRKVTVAVASQK
                     LVASIGTPSINVSVFPGEKVNFKGSGDGSTNLPLTYNWRFTGGSPSSSTAQNPGSVSY
                     ASAGVFAATLTVKDSKGTQSEPATRTVTIVNSNQAAPLLGRCSIGSDDDDDDDDDDDD
                     DDDDHDKDDDKDKDKDKDKD"
     gene            15692..16045
                     /locus_tag="PLB25_01305"
     CDS             15692..16045
                     /locus_tag="PLB25_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40262.1"
                     /translation="MAILVASTVIFATSEALAEAQWRHVGTLGQRHLVLMDVAVQDNV
                     EQLNQAARAVCEPSSPCLVAFWSDVAAVPIAMPMSAAQQQAMVAQYVRNPASGKEELL
                     LKCPPYSAASAKCLR"
     gene            16070..16711
                     /locus_tag="PLB25_01310"
     CDS             16070..16711
                     /locus_tag="PLB25_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HOE40263.1"
                     /translation="MTDQAHSNQDESILVLGIGNILWADEGFGVRAVQALNSQWKLPS
                     RVTLMDGGTQGFNLLTHVQAASRMVVFDAIDYGLPPGSLKVLSNEQVPRFMGSKKLSL
                     HQTGFQEVLAVAQLTGHLPCELVLIGVQPETLDNFGASITQVVKAQIQPALGIALDFL
                     RGWDCEPQARICGLETVEYLQPESLDMIAYESVAGASNADADGLSTFLSSDTR"
     gene            16711..>16898
                     /locus_tag="PLB25_01315"
     CDS             16711..>16898
                     /locus_tag="PLB25_01315"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOE40264.1"
                     /translation="MTKQLANDLAGSQPSVRNYLDATEFIRVRGMVQGVGFRPMVWRL
                     ARAHGLRGWVSNDGQGVI"
BASE COUNT         3520 a         4924 c         4894 g         3560 t
ORIGIN      
        1 gtttcattga caccggctcc aacggcctgt actttgatac cagcacgctg acagcttgca
       61 ccctcagcag tcagatgtac gggttttatt gccccagcag caccaccgag ttgtcggcga
      121 ccttgtcggg caagaacgca gtcagtgcca acgtgtcatt ttcagtttcc aatgcacaga
      181 ctctggtcat cggtaccgat ttgaccgcct tgccttctct ggccgggcag atgggatcgc
      241 gcacggtgtt tgactggggc ttgccattct tttacgggcg cagcgtgttt tttggttttg
      301 accaaaagtc atcaccattg ggtatcgggc cgctgattgc tttttgaatc ctggcatcgc
      361 tgtctgcgca tatttaccct tgaaataccc ctctgacgct tgtcctgctt gcggtgctag
      421 ctatttttgt tgtagcagat atgacgatcg catggcgttt ggtcggcgcg gcttgctgaa
      481 ccgcacgtgc tgccccagtt ggcacaactc agccgtcatg ctttgtgcac ggccggtcat
      541 gctgccgccc gactgatgtg atcagccagt tgcgggcatg gcacggcgaa cggctccagg
      601 ccatggcaat catcggcgtg cgcttggcag ttttggtgat cgcaaggggt ttatgtgcaa
      661 tcgacaacaa acaacaattc atcatctgct gaaataacaa tgtgctgtac agaaaaaatt
      721 caaagtggta tggtcgattt ttcagcacaa aactcatttt caggagtggc aatgtttgca
      781 cccaaggcag tggttctttt tggtttttcg atgtcagttg caatggctgc tacagcagat
      841 acctttccga ccggcaatgt atttgacctc accgtagggg tcaactcggt ctatcagccc
      901 gcaatagatg ccatcaccgg tggcatcgtg accaatccgg ttggtttcac atcggtatcg
      961 gacatgtttg acggtgccaa gctcagcagt ttgatgaaac ttaacagcag ttataccgat
     1021 tcatcaccct ctgtgattcg catgggctac cggggcttgc ccgcattgat cagtaccggg
     1081 acgactccga ccatcaagtt tgaaataccc gcgatcaacc ttgtccaggt atttgatacc
     1141 aaaccaactc gtgatggcaa cctggatgat ctgaacgagt atctgaaaac cagtggcaat
     1201 tcattgcttg accagatgca aaagctgctt gtcaaggtat ctccagtaga cccggtagcc
     1261 ggaaacccca attcgcttca gtcacaaatg atgatggctg attttgatcg tagttttacc
     1321 cagtttgcct cgaatatcaa ggaggccact ggcgaatcca ataatctctt tggtattggc
     1381 ctgtcttttg gcagtttcac ccagcacggt gttaccaata atgcgatgac aatacccctg
     1441 tcctatgtgt ttcgctcgga tattgatccg gcccgccaat tgacccttta tgccccggtg
     1501 actgtgagct cagtcgaagg tgccaagagc tacgcggtca attttggtgc ttcctaccgg
     1561 tatccaatca ctgatgattg ggcactgaca cctggcattg gttacggtat taccggcagc
     1621 tcagatctgg gggcgggggc cgcgatgctg gcactgtcgg tgacaagtca gtatgtgatt
     1681 catcgcgacg gctatgacat cggtatcggc aatgcactgg gcacttaccg gtcccaaaag
     1741 ttttcagcag gtgattattc ctctgacccc ggtatcagca acacggtttt tcgcaatggt
     1801 gtcctgttgt catttccggc catggtctat ggcaagaaaa tggcctatga attctcgttc
     1861 atcaataccc gcttttatgg cagcgatttg tattccgaaa attacaacga aattggcgtc
     1921 acactgggca ccaatcgaag tgccaatagt tcccgcagtt atttgcgtgc cgggtttacc
     1981 tatctgcagg gccaaaacga tattcggggt tatcggctca atgttggtta ttggttctga
     2041 cgtgtttgcc ttgaagtgag ttcactgtgg tcactcaggg tggtctgatt cgcaacctgt
     2101 gccgcgtctt cgggctggcg atatgctgtt ggttcgggtt ggcggcaccg gccagcgcac
     2161 tgactggtaa cgagcaggtt tccctcgctg gcactggcaa gtttgaagcg ctggtcagcg
     2221 gcatggagtt gatgctcgac aagcagccga tgaagtcttc ggactggtac tcgctgtgtt
     2281 acggctactc gcgggtcaag cactacggca agatattgga ctgcctggat tcgcttgaca
     2341 agtcgctggc caagggggac acccgcaccc ggctgtttgg tctggatgat gcgaccgcag
     2401 catcccatct gatgcgcgcc gaagcgctga tcgaattggc cgagtacgac cccgcgctgg
     2461 tgcaggttca aaaggcgctg gattggtatc agcgtgaaaa aagcgatgac aaggacattt
     2521 tcatcaatgc cctggcggcc aaatcgctgg ccaccctggc cttggggcgg cgcgctgaag
     2581 ccgaacgtct atctgccgaa ctcgagggct tttcgctggg catgacgggc ggggattatg
     2641 ttggtgccaa atcgctggcg atggcgcggg tcaacatggc cctgggcaac tggcagaagg
     2701 ttcttgatgc cctggcgcaa gacaagatat ttggcttgcg cagttttctg gacaacctgg
     2761 ccagcggcgc ttttttgctg ggtatcaaca attgggtctg gattgagctg ccgcgcgcct
     2821 ttatgcaaac caaggccttg ctggaacttg gccagcttga tgctgcccgt tcgggctatg
     2881 acaaactgct ggcggtgccg caggtcaagg ccaacggcga gatttactgg atggcgctgt
     2941 atgaccgggg ccgaatcgcc gagcgccaag gccagcttga ggaggcagag cgtttttttc
     3001 aggaggcggc cgaggtgatt gaacgccagc gccgttcgat caacagcgag gccaacaaaa
     3061 ttggctttgt cggggacaag caggcggtct atgccagcat ggttgccaat caatttcgcc
     3121 aaggccggtt tggtcagagc tttgagagca tggagcgcgg caaatcacgc gcgctggtgg
     3181 acctgttggc atccaatcaa agccagcgac tgatctcacc aaaatcggcc aacgcggcca
     3241 aggtgaccca ggtgcttgac cgtatccgca atgccgatct cgacagccgc cgccaggacg
     3301 aaagccggat tcggtcggtg tcgcaagctg ggggttcaaa tgagctgcag acactcgcca
     3361 gcgccctgcc aagttcgctc gcatcgctgg tcagcgtgtc gcggctcgac ctcaaagagg
     3421 cgcaaagtct ggtcaagccc gatgaagcct tgatcgagta ctttgccgct ggagacgagt
     3481 tattcgccac actggtgcaa agcaacagcg ttcatggcgt gcgcatcaat gccagccagg
     3541 tcgagagcgt cgttcgcagt ttgcggcaaa acatgcagga gggctcgccc caggccttgg
     3601 gccagttgca ggccctgcac aagctgctga ttgaaccctt gctgccaaac atcagggtca
     3661 agcatctttc catcgtgcca catggggtgt tgcactattt gccatttggt gccctcaatg
     3721 acggcaagca agatctgatt gaccgattca cgctgcggat attgcccagt ctggcggtgc
     3781 tgaaatacct tcgaccgtcg caaaccaaag gcatcgaacg cctcctggtg ctgggtaacc
     3841 ccgacctcgg acggcccgat ctggatttgc ctagcgccca gaatgaagcc gaaaagctcg
     3901 cggcgaagtt caaggccgag catttgctgc tgcgcaaaaa ggcaacgcgc acggccttcc
     3961 aggcgctggc acctggtgcc gcgttcattc atgttgcttc gcacggccag tttgaagctg
     4021 gaaaaccgtt gcagtcaggg cttattctgg cggcggacaa gcccggcggc ggcatcaatg
     4081 cgggtcgctt gacggtatct gacctttatg aactggcgct tgatgcagag ctggttacgc
     4141 tgtcggcttg tgaaaccggc ctgggcgcag ttgccagcgg cgacgatgtg gtcggcctga
     4201 cgcggggctt tttgtatgcc ggtgtcagca ccgtcattgc atcgctgtgg caggtcgatg
     4261 acgaagccac aacctacttg atgctgcaaa tgtacgaaca cctgcagcgc ggtggctacc
     4321 ccgacgcact gcgcaaggca cagatcgaga cccgcgcaaa atacccgcat ccgatgttct
     4381 gggcggcgtt ctatttgact ggactgaatt gactggcctg aagtcctgtt caaaacaggc
     4441 cagcgttccc caacgaaaat ccggcaattc tggtttgatg ttctgaccac gttttggttt
     4501 gggcgatcaa ttcgagagca cgtttatttt ttggcttggg cgttgtgatg atctcgacag
     4561 tgagtgcctc agccgcatct cccggcgtgg agccattgtg ctgcccaaaa tagcgcgcag
     4621 cccggccatc agggctctta ttgcctttcc aaatcatcca tgtcgttgtg atctcgcctt
     4681 gcatcgcgtt tgcaatgtct gcggcaggat gcctcgacac gaatgaattg gaaatggatt
     4741 tggccacgat catggtcaag actctgtcgc cctgatggcg cagcggctgc gaaccgaccg
     4801 ctgcggccca gttcgcaccg gctttttgac cattagcccg gcgatggatc ggcaagtcca
     4861 gcacagcgtg accttgctgc ggccgcatcc tgctagcaat gtcatacgtc aaaaagagtc
     4921 ccagcccttg ccatcattgc gctggtagct acataaaaag tagcgttatt gatgccttca
     4981 gcaaattcgc ggcagttgct cgccactgag ccagtcaacc actcgccgac cgccaaagcg
     5041 ggtcgccatt tgcacaaaac agttgtcatc agccgtcacc gtgccaatgc gtgcggcgcc
     5101 atgacccaag ggatgagccc gcatggcagc cagcagggtg tcggccgctt cgggcgagca
     5161 gatggcaatc agcttgcctt cgttggccac atacagcggg tccaggccga gcagctcgca
     5221 ggcggctgca acttccggca gcaccgggat atcgccttcg atcaggctca tgccgacctg
     5281 ggactggcgg gcgatttcgt tgagcgtggt ggcaagcccc ccgcgggtcg ggtcgcgcaa
     5341 gacatgcaat cttgccgggt ctggcagcgc ctgcagcatt gcctgcacca gcgtgtgcaa
     5401 ggcggcagtg tcggacttga tcgccgtttc aaacgtcagc gactcgcgct gcgacatcac
     5461 cgccacgcca tgctcgccca gcgtgccaga gaccacaatg gcatcacccg ccctggcctg
     5521 gcggccgtca atcagcagcc cgtcaggcac cacgccgacc ccggtggtgc tgatgaagac
     5581 cccgtcgccc ttgccttttt cgaccacttt ggtgtcaccc gtgacgattg gcacaccggc
     5641 ctggcgcgcg gcgctggcca tcgactcgac gatgcgcttc aggtcggcca aagggtaacc
     5701 ctcttcgatg atgaagctcg ctgccaggta cagcggccag gcacccgaca tggcgacatc
     5761 gttgaccgtg ccgtgcactg acaggcagcc aatgtcaccg ccagggaaaa acagcggcga
     5821 gatcacatgg ccgtcggtgg ccatcaccag ccggcctttt ggctgcggca acaaagctcc
     5881 gtcatgccct tggcgcaggt actcgttgtc aaaggcggcg gcaaacaact cgtcgatcag
     5941 ttgcgcagca gcgcggccac cggcaccgtg tgtcatgtcg acgcgcccgt gtttgaagtc
     6001 aatcgggcgc aggtagtttt ttttgacttt catgctgcca ccaccgcaat gtcctgaaac
     6061 cggccatagg aatagtgtgc cgcgcaagca ccctcgttgg acaccatgca cgaacccatc
     6121 gggctctctg gcgtgcagac ggtgccaaac agcttgcagt cagccgggcg cttgatgccg
     6181 cgcagaatcg tgccgcattc gcattgcttg tggtcgggca cgctggcgta gtgcaaatcg
     6241 aacttgactt cggcatccca ggcggcaaat tcggggcgaa tcttcagggc actggctggc
     6301 acttcaccca aaccacgcca ctcaaaactc tcgcgcaatt caaacacctg ggccatcact
     6361 ttctgcgcgg cgaggttgcc agtggcggtg acgacacggg aaaactcgtt ttcgacttcg
     6421 gcgcggccgc tgttcatctg gcgcaccagc atcagaatgc cctgcatcac gtcaagcggc
     6481 tcaaaaccgg tgatcaccac cggtttgtgg taagtccggg cgaaatggac atagggctgc
     6541 gagccaatga tgatgctgac atgcgccggg ccaatgaagc cgtcgatggg tatggcaccg
     6601 agttcgcgcg cctgggctga ctccagcagg tgtgtgatgg cagccggggt cagcacatgg
     6661 cagcacagca cgctgaaatt ggtcagttgg cgcgcttttg cctcgcgcag caccagcgcg
     6721 gtgggcggtg tggtggtctc aaagccaatg gcgaaaaata ccacttcacg gtgcggatgc
     6781 tgctcggcca gcgccagcgc atcggccgcc gaataaacca tgcggatatc gccaccacgt
     6841 gccttggcct tgatcagtga caggctgtcg gaggccggta cgcgcatcgt gtcgccgtag
     6901 gtgcacagca tcacgccccg gtccagcgcc agcgaaatag ccaggtcaat gcggccaatc
     6961 ggcagcacac acaccgggca gccagggcca tggatcatgc gcacattggc gggcaacatg
     7021 ccgctgatgc catagcgcga aatggcgtgc gtatggccac cgcagaactc catgaagtta
     7081 taggcttggt ccggcagggc ttcggcggcg atcttgctgg cgatcaggcg ggcggcatca
     7141 ccatcacgga attcgtcgat gtatttcatg gcgtgcctgg ttgcttacgt ggtttgcctt
     7201 gctgcgccct accctgagat ttgacgatag agcttttcat gctgcttcca tctgcgccag
     7261 ctcggcaaac agggccagcg tgcgtgccgc ctcgtcgggg tcgatcttgc caatggcgta
     7321 gccgacatgg atgatgacgt agtcatctat ctgtgcgtca tcgaccaggt caatcgacac
     7381 ttccttgcgc acgccaccca gatcgaccac ggcgcgctgg ccgtccaata actgcaccac
     7441 acgggctggc agggctaaac acatgaattt gactccggtt ggggtgttgc ggcgtgatcg
     7501 gcgtgcaact gctgcgcagc gacccaggcc tggcccagcg ccagaccggc atcgccgcat
     7561 gaaatggttt gtggatgcca gacgcgcagg cctgcgtttt caagctgcat cgtcaggtgg
     7621 gttttgagaa tttggttaaa aaagcagcct ccgccgagcg cgatgtctgc gatgctgtgc
     7681 tgtttggccg cccgcatggt ttgctgggcc agcgcggtgg acagtgtcag gtgaaagcgc
     7741 gctgcggctt cgcccacaga ggcggggtcg gcaagattta accctgctga tgcgctgcac
     7801 cactgatttg gcaggtgagc gggttgaccg gcactcgcag gtttgcttgt cggcacgatt
     7861 tcagggctgt agtcaagcca ggtattaaac aagggcagca aactgatttg tcctccctgg
     7921 gccacacacc aatcgtctgc acaaacttcc agcgcctggt cttgcagcca gcgttttgct
     7981 gcctgctcca gcgcgatcgc cgcttgcgcc tcttcggttt gccggaccga caagcccagc
     8041 aacccggctg ctgcgtcaaa ccagcgcccg gcggcggttg tttgcgggca atggatggac
     8101 cgccgcaaca tggctgcaat cgtcttggcg gcctgttggc ccaccaccgg gccaaagcgc
     8161 ggcgcgatca gatccgcacg gccgcaggcg tgcagggcgg cggctgccat gcgccaaggt
     8221 tcacgcgccg ccacgtcacc gccaggcagg gttagcggca gcagatgggc aagccgctca
     8281 aagcccgcaa gattcaccaa tagcaattcg ccaccccagg ccagagtgtc gctgcccagg
     8341 ccgacaccgt cgagcgccag tcccagcacc ggccgggcca ggccatgttc ggccatcagg
     8401 gctgcgatgt gggcgtggtg gtgttgcacg gcgaaagcgg gcacccgcaa ctcgtctgcg
     8461 gtttgtaaag ccagatgggt gctgaaaaag tcggggtgca gatcatgtgc taccgcatcg
     8521 atgcggcctg ccgcctgcgc cagcagtttg gccaccgaat cgcgcagaga gacacaggca
     8581 gcggggtcgg acaggtcgcc atgcacgact gaccagcgta ctgtgtgggg cgcgtcgcta
     8641 tcgaacaggc aggtggtgtt tttcagaaac gccccgaatg ccaggatcag tgccatgggc
     8701 gggctttcag caacgaaacc gccagccttg gtcggcgatg gcttgcaatc ggcgacgaag
     8761 tagtgtggca ttgttgatga tgacccgcaa aatccgtttg agcagaccaa catcgttatt
     8821 tgggcaaagg gcgtagcaga tttggggtcg gctacccatt cggtccggcg tgctggtttg
     8881 gctcaacacg cgcaccctga atgggactct gacaccaatt ctgcgcaact tagccgcaca
     8941 gcttgcagca gtgaaacaca caacgctatc atacaaatag ctatcagcgc aatacccaaa
     9001 ggggctggag ccataaaaac catcagatag aaccatcagg caggtcaagc cggcaacgcc
     9061 cgaagccgca ccaactccgc ctccaactct gcaatacgcg ctgcaacggg attgccattg
     9121 ccagcagcag ggtcggtcag cagccagtcc agccaggtct gcatgccgac accggtggtg
     9181 gcagacagct ccagaatctc gatgccaggg ttgacccggc gcgcaaactc cttgcagcgc
     9241 gccacgtcaa acgacacatg tggcagcaga tcaattttgt tcaggaccat cagtttggct
     9301 gcggcgaaca tgtcggggta tttcagcggc ttgtcgtcgc cctcggtaac cgacaggatc
     9361 gcgaccttgg cggcttcacc caggtcccac atcgccgggc agaccaggtt gccgacgttt
     9421 tcgatgaaca gcagcgcctg attgccagtg gcacggtcat cggcgtggtc gtgatcatgg
     9481 ctatgcgtgt gtccatgccc gtgtgcatga ccatggtcat ggtcatgccc agcatcacgg
     9541 ttgtgctgat gcgaatgcgg gtggtcatga gagtggtcat gagagtgcgc cacctcagcg
     9601 tgctcgtgac cgtgactgtg cgcttgctcc gcatactcgt ggtcatgggg atggtcatgg
     9661 ggatgggtgt taacctgttc ttcggcacca tgcccgcgcc catcatggtg gtgtccactg
     9721 gcgtgcaaat ccagtcgcga aaatgcctcg gccaccatcg gtgcgtccag gtggcagcct
     9781 ttgccggtgt tgacctgaat ggccggtgcg ccggtggcgc gaatgcggtc agcatcaaaa
     9841 ctggtttgct ggtcgccttc aatcaccgcc accgccaggt cgggtcggtg ctggcgcagc
     9901 gcctcaatgg tggcgcacag cagggtggtc ttgcccgagc cggggcttga caccaggttg
     9961 taggcagtga ccccgtgcga ggcaaagtgg gcccggtttt gcgccgcgat caggttattg
    10021 gcccccagca catcggcttc gatcttgatc gcccgttgct ggctcatgcc gggcaccgac
    10081 acccgggctg cacccgcgcc aaagtgcagg tcaccggtac cgggttgcac gttcacgccg
    10141 ggcgcgctct ggtcgccaga agaagtattg ccacatccac aaaccacaca catgttgttt
    10201 tccttgtctt gatgccgcca ttttgacgcg ctacggatcg cgctgcattg ggctcacgca
    10261 tcataggcag cgccaagcct ccctgccgat gcgtgtcttg tagcggccag gcgcgtaccg
    10321 caaaacgcct gtttacctgc catgactcag ctcaaccagt tccgacctgc cgttgcaaat
    10381 gcggggattt gtaaccttgg taagtttggt aacagtatgt aagaccgtat ttttgacggc
    10441 aaatagcccg ttctaatcag cgccatgaac acattgagtg aactttctga caagcagact
    10501 gccatgaatt gcaggccatt catcgccaga ggacccagca tgtcccacac cttgccgccg
    10561 tcattgaatg aggctagaga ccatgcatag cctgattgat gaagctggga tggcgcgaac
    10621 cgctcaaacc agcggttctg cactggcacg ccaggatggt ggtgcccaag cgggcaccac
    10681 ttcagcaaca accagccata tgggcaaaca acatgcgatt ggacagaacc atcccatcat
    10741 ctggctacac cccgaagcac cggtattgtc ggttgatgcg cagcctggga cagtggcgaa
    10801 cgcggtcttt gccgaagtca aaacgagcgt tgagcgcctg gtcgatcagg gagaaacccg
    10861 atcagtcgat cttcgtttca tgcagtccat gcccgctgag cgagcggcat tggaaagcct
    10921 gctgggtcgc ggcgaagtac ttgccgaggt gcatgcggcg ggccgtagta aggttcagga
    10981 aaccgccata ccctgcgtct ggctgatccg tcacttcaac agtgaagatg aattggtcgg
    11041 cgaactgatc gaagttaccg acgttcctga gattcttgtc agcgaccggc aggcggtcac
    11101 tcattgcctg acccagttga atgccaaagc accaaccggc agcaactgaa ctttgaaaac
    11161 tcctccaaac cgcagagagg caaccatatg aaaaaaccgt ccaccgtgtt tgattcgctg
    11221 cagcaacaag gcgtatcccg ccgcagcttt atcaagttct gtaccgtgac tgcatctcgc
    11281 tggggattgc cggaaccagt gtgaaagcat tcactcaggc gctcggtgct gcgcagcgcc
    11341 cctcggtgat atggatgtcg ttccagcact gtaccggctg ctccgagtct ctgatgcgct
    11401 ccggcagtcc attgtcagac cgcctgaccg tagagaacct catcctcaac ttcatctctc
    11461 tggattacca tgagacgctt caggtggcgg ccggtgcggc ggcagaagat gcccggatgg
    11521 cggcaatgaa ggcaaatttc ggcaaataca tcttgatcgt tgacggctcc gtgcccaccg
    11581 ccgaaaacga atggtggctg gccgtcaacg gacattcggg cctgagcctt ctgcacgagg
    11641 cagtcgctgg tgcagggctg gtactggccc taggggcttg cgccaccttc ggggggattc
    11701 ctgcggcgtt tcccaaccca tcgaaggcaa tgggggtaag cgacctgatg gacaagggca
    11761 tgatcgataa gaagcccctg atcaacatat cgggctgtcc gccaatacca gaagtcatca
    11821 ccggcaccat cgcgtactac ttggtgaacc aggcgatgcc gccgcttgac gcgctgggcc
    11881 gtcccctggt gttttttggc aagacggttc atgactgctg cccgcgtctg gaccatttca
    11941 acgccgggga gttcgcgacc tcgtttgatg acgatggtgc acgcaagggg cattgtttgc
    12001 tcatgcttgg ctgcaagggt cctgacacct tcaacgcctg caccaccacc aaatggaatc
    12061 aggggaccag ttccccaacc caggcagggc atggctgcat cggctgctcc gagcccaact
    12121 tctgggacaa gcccggcggg gtctatggat tcagttcggg gttcaagaca ggcgacggaa
    12181 cctgtgccgc accagttttg ccgtcgcttc cattgatcta gccctggcac gtctcgccag
    12241 cacaacagtt tgaggagttc aaaagtatga gtacgatcgt cattgatcca gttacacgca
    12301 ttgagggtca cctgcgcata gaagccgaaa ccgacaagtc tggcgtggtc accagcgcgt
    12361 tgagcagcgg caccatgatg cgtggcatcg aaatcatcct caagaaccgc gatccacgtg
    12421 aagcctgggc gtttacccag cgaatctgtg gagtctgtac cgtggtgcat ggcatgacct
    12481 cggtgcgtgc ggtcgaagat gcgctgggct acaccattcc aaccaatgcg cgccacattc
    12541 gcaacctgct gatgggctcg caactggtgc aggatcaggt gacgcatttt taccagctca
    12601 cttcgatgga ctggatcaac gtggtgtcgg ccagccaggc caatcccacc accacagccg
    12661 agttgcaaaa atgtcactca aagtggccca acaacacatc agcctatttt gagtcggtgc
    12721 ggcaaaagct cgccaatttc atagcgagcg ggcagctcgg tctgttcagc aatggatact
    12781 gggacaaccc gggctacaag ctgccacctg aaatgaacct gatgctgttt gcgcactggc
    12841 tggaagctct ggagtggcaa cgcaaggttt tacgcatgca ggcgattttt ggtggccgca
    12901 acccgcaccc caacttcacc atcggcggcg tggcgtgttc gttccatatg gctcccaacg
    12961 cggccggcat tctggtgccg gcaccgggtt ccaccggtgt cgatcaagcc gggcttgacc
    13021 tggttcaatc ctgcattacc gacatgcgca actttgttgt gcaagtgctg cgacccgacg
    13081 ttttggccct ggcgagctac tacaaagact ggttcactcg gggtgaaggt gtgggcaact
    13141 ttctcaccta tggtgagtac ccgcaggacg acaatgccga tttggcaaag aatctgtttg
    13201 tgccccgcgg ggcgattctc gaccgcaacc tgtcgaacat acagtcggtg gatctacgca
    13261 agacggacga ggtgcaggaa ctgattcctt caagctggct tgggtatacc aaggggaaaa
    13321 gcatcggtct gcatcctttc cagggcgaaa ccacgccaga ctacaccggt cccaaagcac
    13381 cctacgacaa gctcgatgcc agtaaaccgt attcttggat gaagacaccg cactggaagg
    13441 gccatcacat ggaggtcggc ccgcttgccc gcgtgctggt aatgtatggc aagcgaaccg
    13501 catccgtgcg cgatcatgtg gacagcgcct tgaaagacct cggcttgtcc ttcgatgcca
    13561 tgtactccac catgggacgt atcctggcgc agtcgattga cacccatgtt gtggcggacc
    13621 agcttcaggt ttggttcaac caactcacag ccaatgttgc cggcggcgat ttcaaaaccc
    13681 acaatgcggc gaagttcaac ccctcaacct ggccagccag ttgcaagggc gtaggcttca
    13741 ccgaggcacc acgcggcgcc ttggggcatt gggtgacgat tgatgccgcc aagatttcca
    13801 attaccagtg cattgttccc accacctgga atgccgcacc gcgagacact gggggcaagg
    13861 gtggcccata cgagatggct ttgctgaaac atactctggc agtgtccagt cagccgctgg
    13921 agttgctgcg caccattcac agcttcaatc catgcatggc ctgtgcagtc cacatcatgg
    13981 atccgaggca ggacgagacc cttaaggtca ccgtttgcgc ctgagcattg cgttattgac
    14041 ccaaggaacc atcatgaaca agatgtcggc atcgtcacgc ttttttttgg ctatctggtt
    14101 tgctgtactg tgtgcgttca ccggtagcgc gctggccttg ccaagttttg gcactcagat
    14161 cgataactat tgtgcgctgc aaccgaccct ggctgcaaca ccccagcaga gtcgccccta
    14221 ccaggcttca gggtcgtcct gcgcaagctg ccacacctcc aacagcccga aaaaaactga
    14281 tctgaacgcg cttggtacgg ccagcaaaac ctgcaccggt tcgacttgtg gtgcttcggt
    14341 gaagccgttc tgcatcgcca ctgcgccgag caacgccagc atcacggcac cggccgctgg
    14401 cagcagcgtc gcgttgggcc agtccatgac ctttacggct ggcaatgcga ccaatcctga
    14461 tggtttccct ctgacttaca aatggacctt ttccagcggg caagcgagcg tcaccgggca
    14521 gagtgtggct gtccccatga gcgttgccgg acctgttacg gcgacattgc aggttctcaa
    14581 ttcggtttca atgctcgcca gcggcacagc gccgacccgc gctgtcaccg tcaccggcgc
    14641 accgaccgga aaccaggcac ccagcgcggc catcgggtcc cctgcagcaa acaccaccac
    14701 cacccctggc ggcacggtga atttccaggg ttcgggtact gaccctgaca acaacgtgcc
    14761 tttgagctat agctggacct tccccggtgg cagcccgtcg agttccgtcg tgcaaaatcc
    14821 tggtgcggta acctatgcca cagcgggcac cttcagcgcg agcctcaccg tgcgcgacag
    14881 ccttggcctg gcttcggtgg cagtcacgcg gaccgtcact gtttcgactc cgggggccaa
    14941 ccaggctcct accgccatca tcagttcccc ggcggcagat acggccgtca taccgggcgc
    15001 tagcgtcaac ttccaaggct ctggcactga tcctgacaac aacaccccgc tgacttacaa
    15061 atggaccttc tcgggcggca gtccagcgac atccaccgca caaaatccgg gcacggtgac
    15121 ctacagtgcc gcaggcacct atacggctag cctgacggtt accgacagta aaggccttgc
    15181 atcaaccgcc acgtcgcgca aagtgacagt tgccgtagcg agccagaagc tggtggccag
    15241 catcggcacg ccgtctatca atgtatcggt gttcccgggc gaaaaggtca acttcaaagg
    15301 ctccggtgac ggttccacca acctgcccct gacttacaac tggcgcttca ctggcggcag
    15361 cccgtcgagc tctaccgccc aaaatcctgg atctgtaagt tacgccagcg cgggtgtctt
    15421 tgccgccaca ctgactgtca aggatagcaa gggaacccag tctgagcctg caacccgaac
    15481 cgtcaccatt gtcaactcca accaggctgc gccgctgctc ggtcggtgtt ccataggaag
    15541 cgacgatgac gatgacgatg atgatgacga tgatgatgat gatgatgatc atgacaaaga
    15601 cgatgacaaa gataaggaca aagataaaga taaagactga gcaagcacaa gctaatcaat
    15661 tctgaaaggg gctttagcaa tgagaaatag cgtggcaatt ttggtggcat cgacagtaat
    15721 tttcgccaca tccgaggccc tggccgaggc tcaatggaga cacgtcggca cgcttggcca
    15781 gcggcatctg gtattaatgg atgtcgcagt gcaagacaac gtggaacaac tcaaccaggc
    15841 cgccagggca gtctgcgaac cctccagtcc ctgccttgtg gcgttctggt ccgatgtggc
    15901 ggcggtgcct attgccatgc ccatgtcggc agcgcagcag caggccatgg tggcgcaata
    15961 tgtccgcaac ccggccagcg gcaaggaaga gctgttgctg aagtgcccgc cctacagcgc
    16021 cgccagtgcc aagtgcctgc gatagcgcct tgatagcagg acaaatacca tgacggatca
    16081 ggcgcacagc aatcaggacg agagcattct ggtgcttggc atcggcaaca tcttgtgggc
    16141 tgatgaagga tttggcgtgc gtgccgtaca ggcactcaac agtcaatgga aattacccag
    16201 ccgtgtcact ctgatggatg gtggtacaca gggtttcaac ctgctcaccc atgtgcaggc
    16261 cgccagccga atggtcgtgt tcgatgccat tgactacggg ttgccacctg gctcactcaa
    16321 ggttttgtca aatgaacagg taccgagatt catgggctcc aagaaactca gcctgcatca
    16381 gaccggattt caggaagtcc tggctgttgc tcaactgacc ggccacttac cctgcgagtt
    16441 ggtgctgatc ggtgttcagc cagaaacgct tgataacttt ggtgccagca tcacgcaggt
    16501 tgtgaaagcc caaatccagc ccgccctcgg gatagcgctt gattttctgc gaggctggga
    16561 ctgcgagccg caagcgcgca tctgcgggct tgaaacggtc gagtacttgc agcctgagag
    16621 cctcgacatg atcgcttatg aatcagtagc tggcgcgagc aatgctgatg ccgatggact
    16681 ttcgacattc ttgtcctctg atacccgctg atgaccaagc agctagccaa tgatcttgcg
    16741 ggcagccagc cgagtgtccg caactacctg gacgccaccg agttcatccg cgtacggggc
    16801 atggtgcagg gcgttggttt tcgacccatg gtttggcggc tggccagggc gcatggcctg
    16861 cgtggctggg tgagcaatga cggacaaggc gtcatcat
//
LOCUS       DAOSLL010000016        16738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_3625_length_16738_cov_4.506324, whole genome shotgun sequence.
ACCESSION   DAOSLL010000016 DAOSLL010000000
VERSION     DAOSLL010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 16738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16738
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3625_length_16738_cov_4.506324"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            387..2387
                     /gene="htpG"
                     /locus_tag="PLB25_01320"
     CDS             387..2387
                     /gene="htpG"
                     /locus_tag="PLB25_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HOE40265.1"
                     /translation="MSKQTLSFQAEVAQLLHLVTHSLYSNKEIFLRELVSNASDACDK
                     LRFEAINNTGLYESDSELKVKVTFDKDAKTLTITDNGVGMSEIEAVEHLGTIAKSGTK
                     DFMSKLTGDQKANASQQGQLIGQFGVGFYSGFIVADKITVESRRAGLPTTEGVRWVSA
                     GAGNFEVELIDRAERGTSVTLHLRDDAMDYCSAWKIKSIINKYSDHISLPILMEKEEW
                     KDGELINPNDEKGGRQPGGMVKTGEWETVNKANAIWARAKKDISADEYTEFYKQISHD
                     MEAPLAHTHNRVEGSTEYTQLLYIPGKAPFDLYNREKSAGVKLYVKRVFIMDDAESLL
                     PTYLRFVKGVVDSSDLPLNVSRELLQESRDVKAIRDGCTKRVLGMLEDLAKSDKLPEA
                     APSADGITDVVSEEDKAQAEKNLGKFTKFYSEFGAVLKEGLGEDFANKDRIAKLLRFA
                     SSTTDAVSVSFADYKARMKEGQEAIYYITADTLAAAKNSPQLEVFKKKGIEVLLMTDR
                     VDEWALNYVHEFDGTPLQSVAKGQVDLGKLQDETEKKAAEEAAETFKPLLAKLKEALK
                     DKAEDVRVTTRLVDSPACLVVQDHGMSTQLARMLKQAGQTAPNVKPVLEVNADHPLVK
                     KLDGSVHFNDLAHILFDQALLAEGGMPDDPAAYVKRVNALLV"
     gene            3156..5117
                     /locus_tag="PLB25_01325"
     CDS             3156..5117
                     /locus_tag="PLB25_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE40266.1"
                     /translation="MQHRLSLIQKFVILGLIALVVAALPALLYFNHSFSQQKKAALEK
                     RGSAPLIALNRMIQLTQTHRGMSASMLSGNETLAARRPALKDKLDQALDAADAEFKAS
                     AVSSAHMTHWQSLRQSWRTLEQSVAGKQIKAAESTRLHTELIAAELLVSEELLVEFGL
                     NLDADIGTAYLIRASLVDMPWLAENLGVMRAAGSGFLTQTTLPPEGRATLQALQKRVL
                     ELQGQMFRNLKVSTAADATLRAALESKAQASHAAVDKALALANQVLIGATEINYPAPD
                     YFDEFTRTIDGLFAFNADAMQMMDKALQERADASRRGMLLVLVCMVIGLGCGAALSLY
                     FVRSITGPVSDAVKVARAVADGNLAVEVPTLGTNELGQLMAALADMRAHLEQVVTAVM
                     HGSESVATASAEIAQGNTDLSDRTEQQASALEQTAASMEQLGSTVSKNADSANKANQL
                     AQSASTVAVRGGDVVGQVVHTMRDINNASRKIADIIGVIDGIAFQTNILALNAAVEAA
                     RAGEQGRGFAVVASEVRSLAGRSADAAKEIKALITASLERVEHGTTLVDRAGTTMTEV
                     VDAIKRVTAIMGEISSASSEQSIGVAQIGEAVDQMDQVTQQNAALVEEMAAAASSLKS
                     QAQDLLTTVAVFHLPGHGTIGHRIVPRLG"
     gene            5169..6728
                     /locus_tag="PLB25_01330"
     CDS             5169..6728
                     /locus_tag="PLB25_01330"
                     /inference="COORDINATES: protein motif:HMM:NF023273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3365 domain-containing protein"
                     /protein_id="HOE40267.1"
                     /translation="MAPSHHTVLPAAIRLRRYWLIFLVLWAASVGWSLQSHLKDIELT
                     SVQVATQGARDMFRMVVLTRNWNASHGGVYVHVTPATQPNPYLRHPRRDVITTDGVAL
                     TLVNPAYMTRLVGEIARIDSGAIFRLTSLKPIRPLNEPDAWERQALTGFEAGAKEAIS
                     VESSKDGALLRYMAPLRVERPCLHCHAEQGYELGSIRGGISVSQRYAPIEAAIRANTH
                     QSTLIHAAVFVLIAALGWSLLELLRRRWFELIGKIQELEMTRGELVQSEKMASLGRMV
                     AGFAHEVNTPVGVAVGAISQHEETLARINTLLGQDEVDEATLREALDHLRHGGALALS
                     NLRRAASLVQSFKRTSIDQSSDQVRRFDMRELINDVCFTLQHTLKRLPIAIHIDCTDH
                     LAVKGVPGLLEQLLTNLIMNAVQHAFDNGARAGNIHISVMLEQGQVLMRFADDGVGMS
                     VEQMARIFEPFYTTRRASGGSGLGLYICYNIVTVQLRGTIVCDSQPNVGSTFTIKFAQ
                     EILPLQEENQP"
     gene            6725..8287
                     /locus_tag="PLB25_01335"
     CDS             6725..8287
                     /locus_tag="PLB25_01335"
                     /inference="COORDINATES: protein motif:HMM:NF023277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HOE40268.1"
                     /translation="MKLIRTRSEPAASADHPAGPHHHWKLLVVDDEPDMREVTRLNLN
                     GFRFADRDLNILEASSAYEARELLAAHKDIAMALIDVVMETDDAGLRLVEYIRKDLKN
                     LMIRLIIRTGQPGLAPERFVIDHYDIDDYKDKTELTASRLYTAVRAALKSFRDLRTID
                     LNRQGLARVLEAAPDIYRITNRSLQQFFQGVLTQIIGLCNLSDSSFISAVGGIIATFD
                     ERDVTVQASSGNLAAQERFENIRAQCSEAVLTGQMPAGLRKDAYVVPLLVQRKPAGFV
                     YIEPTQELSDADRGLINMVAQQCSNALENLRLHIDLARSHDHMADMLAKVAEFKDHTT
                     GSHIRRIDHYTRLVAIELGISPFESSLYGKASRLHDVGKVGIPDAILGKPGKLDAAEF
                     DLMRTHTHIGGIILERDRFLGVACDVARHHHERWDGKGYPDSRPSREFHLVTRIVSVV
                     DVFDALVSPRPYKEPWPAERAAREIEAGAGTQFDPTVVVAFLKLFRQGKFDHIVGASQ
                     QATDLPDPLDEM"
     gene            8372..8815
                     /locus_tag="PLB25_01340"
     CDS             8372..8815
                     /locus_tag="PLB25_01340"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOE40269.1"
                     /translation="MTQKATLMLATASLICLPAAWAADATLDAAAKEPGAIVTASGLV
                     YLSTKNGTGDSPKASDKVKVNYRGTFPDGREFDSSYKRNQAIEFPLSGVIKCWTEGVG
                     LMKVGGKAKLTCPASIAYGERGAGGVIPPNATLLFEVELLGINGK"
     gene            complement(8958..9104)
                     /locus_tag="PLB25_01345"
     CDS             complement(8958..9104)
                     /locus_tag="PLB25_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40270.1"
                     /translation="MRFQIDATLGAKPQTVPQHGTAKQQHGALIWKWHEALVTIAKTE
                     ALGG"
     gene            complement(9165..11939)
                     /locus_tag="PLB25_01350"
     CDS             complement(9165..11939)
                     /locus_tag="PLB25_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOE40271.1"
                     /translation="MLQQAVLVVLLAACCLIALMLVIFVQRLARLRWQRTRCHSVVCE
                     DVPASSRTILDLPAQQLAELGFVYRYSGAARRALVSTKDEPLFFDVYQHIDGHTHAMV
                     SLAPVPDPRQPCMIQLVTSLADGSNWTTLNRCRHYSPMTMPNWQVFDDYLPQWAQAWQ
                     AHRQRVQMLPLPDLCTDGIEIKRRLDQTFEELIPQMLRQGQLVPIANSKHYRLTWLAA
                     LRFAAIALVGQWRAAWATRGLPPATGYAGSADGLAADLQAFEEQLALRRAATSGGTAK
                     LLMFVVSAALFLVVGGWWFSWSFVPVLLAVIALHEGGHFLAMRLTGYRNVSVFFLPGL
                     GGLATGEKATATPMEKLFVYLAGPVPGIVMAGAAFWASSAGHWLAPGWLNEFLIASLA
                     INYLNLLPITPLDGGRVLETFAFARHPRWRFGFAVLCCCLLLAGGIALDDLVLKVFAV
                     LLAIGLSHQWHLMRLDLAVARDDANGVDEPQALRRIFAALQMAPFRAWSFARRTAVAT
                     ALLPELMGRRPRRSEAFVGLLIYAVCLIGPIAAALVAVPQLGAVLPYMASGLTMAGDD
                     VDPEPADPPAAQPVNWNARLAQAANLSREDLLQAYLGAGQEANGVEDRDTAERHFRLA
                     WEIAKDLPLRDLRRIDALEGLAAATDSDLEQHDNLSRIVAELVQPQGAERVRVASAKE
                     QLSYGDLPAPERVALLRDAVALRAAAMPALDHSLSYTRLALANALDANADSAGAEAVL
                     QTRIDSLPVPDAGDRTRKGLQNALAGVLSRIDLAWFLIDHGRATDAMQMAALALQNLP
                     AKVTVSWTGPQQQVLEVLVWAHLLAPPTPALAVHWKAYDQALRNRFAGGRKLLFHEAD
                     RALVAQALQDANLQASAKKGVQEAMSTMAQRPAMLCQPPQATQRPNWRRLQQDGRRRI
                     LADLGGCGPA"
     gene            12739..13320
                     /locus_tag="PLB25_01355"
     CDS             12739..13320
                     /locus_tag="PLB25_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40272.1"
                     /translation="MIHRLDALTFACLMALLGCGGGTESAASPPVVEVPVLNASFDQV
                     SATGALVSWLATEHSNGQSYTFQADSKGAWSAPSSAKIHRYGLETFGLLNQHIQLQPA
                     WINKTARLSAQLKTEGATDGGGALVLQMIGSRGDILAWNHMVDARAKQTQDWKQYSID
                     LKVLSTALTINVGVMLEGAGTLWADDIKLEIID"
     gene            13546..14952
                     /locus_tag="PLB25_01360"
     CDS             13546..14952
                     /locus_tag="PLB25_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HOE40273.1"
                     /translation="MLTPLKQLIRHSSQRQRFLAEVRALWLLAWPILVGQLATVGMAV
                     VDVAMAGHASAQDLAGVSLGVSIWNMLIITLMGLMMSVNPMVAHQVGAGDLSGVPHVV
                     RQALWQALGLGLLAGLLANWAALLFDHLTLEPPVRVLAIKFVFVTSLALPLFACYRVL
                     YGYSTSINQTKPLMVIALLSLLLNIVVNSLLVFGALGFPRLGGLGCAWATLITVAFNL
                     FALLWWMHQSSAYRSTWPFGHFEPPQWRQISSLLKLGLPIGITYFAETSAFSLIALLV
                     AEFGSTQMAAHQIALNFTSLVFMVPLSLGLALLTRVGQSLGAGDAVQARYQAWVGVAL
                     GLGFAALSALLIASFSTAIASAYTSDAAVIGLAAQLLFLAALFQLSDATQVVTSCAIR
                     AYKVTRSPMVIHLTAFWVFSLPLGYALGIAPNWLAWAPARPMAAQGFWIALVVGLTIA
                     ALGLVWLLRRVARSRLPA"
     gene            complement(15289..>16738)
                     /locus_tag="PLB25_01365"
     CDS             complement(15289..>16738)
                     /locus_tag="PLB25_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE40274.1"
                     /translation="KNGEVFPKWLSISAVKDRHGAVTHYIGAYIDISERKLAEEKIRK
                     LAFFDQLTGLPNRILLLERLQQTTAANARVGAYGALLFLDLDNFKELNDTRGHASGDV
                     LLIQVAQRLSDNLRPGDTVARLGGDEFMVMLSGVEASTLAAAAIEVELACQKLLAALA
                     QPYELLNADFHCSASIGITLFRGQETSVDDVLRQADLAMYQAKSLGGNTFVFFDPVME
                     SSALEHARLESELRQAIVAGQLELYYQPQVSGSSGQIEGAEALVRWNHPQRGLVPPND
                     FIAHAERTGLILPLGQWVLETACQQLAAWAGRPDMAHLTVAVNVSAQQFKLPDFAEQV
                     IATLTRTGAAPQQLKLELTESVFAGNIDEIVAMMVKLKALGIGFSLDDFGTGYSSLAY
                     LSRLPLDQLKIDRSFVTNIELGDNNMVICAATISLAHSLRLKVVAEGVEDNAQRYFLS
                     TVHRCDLLQGYLYSRPVPQQAFEKLVATWRAR"
BASE COUNT         3503 a         4988 c         4935 g         3312 t
ORIGIN      
        1 cacaggcgtt tcgtccgcaa aaaatgagcg ccatccagcc acggagctgc atggcgcttt
       61 ttttcaatct gcaccaactg tgtgtagcca cccgtgagtg tcaacatcac attcctgggt
      121 ggtgctggca ctgtcgccgg cttcaagtac ctggtgcagc ttgcggcaag cgtctgttgg
      181 tggactgcgg gctgtttcag cgctacacat gggacaacgc aaaccaacca agtcgctagc
      241 tgagtcaagc gggcaaaaca cgtgaccggt gtactgagct gaacatgccc cttgaaatat
      301 gcgatatcac ccctagctaa caggtcgctt gagtgtcggc gcgtctgtcg ccggttttat
      361 taatgccctg agaacacgca taccccatga gcaaacaaac cctttccttc caggccgaag
      421 ttgcacaact gctgcacctg gtgacccatt cgctgtactc caacaaagag atcttcctgc
      481 gcgaactggt ctccaacgcc tcggatgcct gcgacaaact gcgctttgaa gcaatcaaca
      541 acaccgggct ttatgagtct gactccgaac tcaaggtcaa ggtcactttt gacaaggatg
      601 ccaaaaccct gaccatcacc gacaacggcg tcggcatgag cgagatcgag gcggtcgagc
      661 acctcggcac gattgccaag agcggcacca aagatttcat gagcaagctc accggtgacc
      721 agaaggccaa cgcatcgcag caaggccagc tcattggcca gtttggcgtg ggcttttatt
      781 caggctttat cgtcgctgac aagatcactg ttgagtcccg ccgggccggt ttgccgacca
      841 ctgaaggggt acgctgggtc agcgccgggg ctggcaattt cgaggtggaa ctgattgatc
      901 gtgccgaacg gggcaccagc gtcacgctgc acctgcgcga cgacgccatg gactattgca
      961 gcgcctggaa gatcaagagc atcatcaata agtactctga ccacatcagc ttgccgatcc
     1021 tgatggaaaa ggaagagtgg aaagatggcg agctgatcaa cccgaacgac gaaaagggcg
     1081 gtcgccagcc tggtggcatg gtgaaaaccg gcgaatggga aaccgtcaac aaggccaacg
     1141 cgatctgggc gcgtgccaag aaggacatta gcgccgatga atacaccgag ttttacaagc
     1201 agatcagcca cgacatggaa gcgccgctgg cccacaccca taaccgggtc gaaggcagca
     1261 ccgagtacac ccagttgctc tacattccgg gcaaggcacc gtttgatctg tacaaccgtg
     1321 aaaagtcggc tggggtcaag ctgtatgtca agcgtgtttt catcatggac gatgccgaga
     1381 gcctgctgcc gacctacctg cgctttgtca agggtgtcgt cgattcatcc gacttgccat
     1441 tgaatgtgag ccgtgaactg ctgcaggaaa gccgcgatgt caaagccatc cgcgacggct
     1501 gcaccaaacg cgtgctgggc atgctggaag acctggccaa gagtgacaag ctgccagagg
     1561 ccgcaccgtc agccgatggc atcaccgatg tcgtcagcga agaagacaag gcgcaagccg
     1621 agaaaaacct tggcaagttc accaagttct acagcgaatt tggtgctgtg ctgaaagagg
     1681 gtctgggcga agactttgcc aacaaggacc gcattgccaa gctgttgcgc tttgccagca
     1741 gcaccaccga tgccgtcagc gtcagctttg ccgactacaa ggcgcgcatg aaagaaggcc
     1801 aggaagccat ctactacatc accgccgaca ccctggccgc agccaagaac agcccgcaac
     1861 tggaagtgtt caagaaaaag ggcattgagg tgctgttgat gaccgaccgt gtcgatgagt
     1921 gggcgctgaa ctatgtgcat gagtttgacg gcacgccgct gcaaagcgtg gccaagggcc
     1981 aggtggacct gggcaagctg caggatgaaa ccgaaaagaa ggctgccgaa gaagctgccg
     2041 agaccttcaa gcccctgctg gccaagctga aagaggcact gaaagacaag gctgaagatg
     2101 ttcgcgtgac cacccggctg gtcgattctc cggcttgcct ggtagtgcaa gaccatggca
     2161 tgagcacgca actcgcacgc atgctcaagc aggctggcca gactgcgcca aatgtgaaac
     2221 cggtgctcga agtcaatgcc gaccatccgc tggtgaagaa actcgacggc tcggtgcatt
     2281 tcaacgacct ggcgcacatt ttgtttgacc aggcgctgct ggccgaaggt ggcatgcccg
     2341 atgacccggc ggcctacgtc aagcgggtga acgctctgct ggtttgagtt ttataaagga
     2401 aataacgctc tagcccttgt ctctattgcg caagaagcta tttaaagcat agtaaaataa
     2461 aattctgaaa gccccgcagt caagctgact ggcggggctt ttgaccattt aattgaatga
     2521 tgtcgcacag ccgcgatgtg gcgttcgccc gtagcattca tatccggcag tctgagaaat
     2581 cgttcaagac cggaaagtct tgactgagaa catgcaagat gttgcgcaat tgcgcactca
     2641 tccccaaagc cgatggcaga cagcgcgcgc taggaatacc cgaccggagt cgcggatggt
     2701 gatgatgtaa aaaaaaatgg catccggctc ctggccggtg gtcgactggt cgggcacatt
     2761 gcgcggcttg acctgaactt cagattgtca ggcgggggtg tgctgatggc gcagccatct
     2821 gatcggcgaa gctcatccag acgacattca cccggtaaaa attcctgaag cgctcggaaa
     2881 aatctggcca tacgctagga ttttgggcga taagtttcag atttggatga ctgacatggc
     2941 ttgctttccg ttataggatg aacgttagcg acagaaggat aagctgccga cttgccgact
     3001 tgccaggttg gcctcatccg gttggagttt caattcgttg gttgaatttc ctgtcctgtt
     3061 tgcgcggaat attcaatgaa ttggtggcta gacgcaaccg tcggtcactg gtcgcattgt
     3121 gatggcggga ttcaaaatag gtaaaggcgt gctctatgca acaccgactc agtctgattc
     3181 agaaatttgt gattttgggt ttgatcgccc tggtcgtagc cgcattgccc gcgctcttgt
     3241 atttcaacca ttccttttcc cagcaaaaaa aggccgcatt ggagaagcgc ggcagtgccc
     3301 cgctgattgc actgaacagg atgatacagc tcacccaaac gcatagaggc atgtcggcta
     3361 gcatgctcag tggcaacgaa acgctggccg cccgccgccc ggcgctcaaa gacaaactgg
     3421 accaggcctt ggacgctgcc gatgctgaat tcaaagcttc cgccgtgtca tcagcccaca
     3481 tgacccattg gcaaagcctg cgccagagct ggcgcacgct ggaacaatcc gtggcgggca
     3541 aacagatcaa ggccgcagaa agcaccaggc tgcacaccga gctgattgcc gcagaactgt
     3601 tggttagtga agagctgctg gttgaattcg ggctgaatct ggatgccgac atcggcaccg
     3661 cctatctgat acgtgccagc ctggtggata tgccttggct ggcggaaaac ctgggcgtga
     3721 tgcgcgccgc gggcagcggc tttttgaccc agaccacact gccgccggaa ggacgtgcca
     3781 cccttcaggc gctgcaaaag cgggtgttgg aattgcaggg ccagatgttc agaaacctga
     3841 aggtttccac cgccgccgat gccacgctgc gggctgccct ggaaagcaaa gcccaggcaa
     3901 gccacgcagc cgtggacaag gcgctggccc tggccaacca ggtattgatt ggtgccactg
     3961 agatcaacta ccccgcgccg gattactttg acgagttcac ccgcaccata gacggcctgt
     4021 ttgcattcaa tgccgatgcc atgcagatga tggacaaggc gctgcaagag cgtgccgatg
     4081 cgtcccggcg tggcatgctg ctggtactgg tgtgcatggt gatcggcctg ggatgcggtg
     4141 ctgcactttc cctgtatttt gtgcgcagca ttaccgggcc ggtgagtgat gctgtcaagg
     4201 tggcgcgcgc cgtagctgat ggcaatctgg cagtcgaggt gccgacgctc ggaaccaatg
     4261 aactcgggca gcttatggcg gccctggcag acatgcgcgc ccacctggaa caggtcgtca
     4321 ctgctgtcat gcacggctcc gagagcgtgg ccaccgccag tgccgaaatc gcccagggca
     4381 acaccgacct gtcggaccgc accgagcagc aggccagtgc gctggagcag accgccgcct
     4441 caatggagca acttggttcc accgtcagca agaacgccga cagcgcgaac aaggccaacc
     4501 agttggcgca aagcgccagc accgtggcgg tgcggggtgg tgacgtggtg ggtcaggtgg
     4561 tgcacaccat gcgcgacatc aataacgcct cgcgcaagat tgccgacatc atcggcgtga
     4621 ttgatggcat tgcctttcag accaatatcc tggcactcaa tgccgcagtg gaagcggcac
     4681 gcgccggcga acagggccgc ggctttgcag tcgtggccag cgaggtgcgc tcactggctg
     4741 gccgctctgc tgatgccgcc aaagagatca aagcgctgat caccgccagc ctggagcgtg
     4801 tggaacatgg caccactctg gtggaccgtg ccggcaccac catgaccgag gtggtggacg
     4861 cgatcaagcg ggtgaccgcc atcatgggcg agatcagctc ggccagcagc gagcagtcaa
     4921 ttggtgtggc acaaatcggc gaagccgtcg atcaaatgga ccaggtgaca cagcaaaacg
     4981 ccgcgctggt ggaagagatg gctgccgctg ccagcagcct gaagtcacag gcgcaagatc
     5041 tgctgaccac ggtggcggtg tttcatctgc cgggacacgg caccatcggc caccgcatcg
     5101 tgccgcggct gggctaagga catggtcatc attcatcaaa ctcaaccggc ctgccacgcc
     5161 acaacatgat ggctccatcg catcacacgg tccttcctgc cgccattcgc ctgcggcgct
     5221 actggctgat atttttggtg ttgtgggcgg catcggtagg ttggtcgctg caatcgcatt
     5281 tgaaagacat tgaactcacc agcgtacagg tggccacgca aggtgcccgc gacatgtttc
     5341 gcatggtggt actgacgcgc aactggaatg ccagccatgg cggcgtttat gttcatgtca
     5401 caccagccac ccaacccaat ccctacctgc gccacccgcg tcgcgacgtg atcaccacag
     5461 atggcgtggc gttgacgctg gtcaaccccg cctacatgac gcgcctggtc ggcgaaattg
     5521 cccgaattga cagcggcgca atttttcgcc tgaccagcct caagccaatt cggccgctca
     5581 atgaacccga tgcctgggag cgccaagcgc tgaccggctt tgaagcaggt gccaaagaag
     5641 ccatttcagt ggagtccagc aaagatggtg ccctgctgcg ttacatggca ccgctgcgag
     5701 tggaaaggcc ctgcctgcac tgtcatgccg agcaaggcta cgagcttggc agcattcgcg
     5761 gtggcatcag cgtatcgcaa cgctatgccc cgattgaagc cgccatccgg gccaacacac
     5821 atcaatcgac cctgattcat gcggccgtgt ttgtgctgat tgcggcactg ggctggagcc
     5881 tgctggaact gctgcgcagg cgctggtttg agctgattgg caagattcaa gagctggaga
     5941 tgactcgcgg agaactggtg caaagcgaga aaatggcctc gttggggcgc atggtggccg
     6001 gctttgcgca tgaggtcaac acgccggtgg gagtggctgt gggggccatt tcccaacatg
     6061 aagaaacgct ggcgcgcatc aacaccctgc tgggccagga cgaggttgat gaggccacgc
     6121 tgcgcgaagc actcgatcac ctgcgccatg gcggcgcgct ggcgctgtcc aatctgcgcc
     6181 gcgcagcctc gctggtgcag agcttcaagc gcacctccat tgaccagagc tccgaccagg
     6241 tgcgccgttt tgacatgcgc gaactgatca atgatgtgtg ctttaccctg cagcacaccc
     6301 tcaaacgctt gcccattgcc atccacatcg attgcaccga ccacctggca gtcaagggtg
     6361 tgccaggttt gctggagcaa ctgctcacca acctgatcat gaacgcggtg caacacgctt
     6421 ttgacaacgg cgcgcgtgcc ggcaacatcc acatctctgt catgcttgag caaggccagg
     6481 tgttgatgcg ctttgccgac gatggtgtcg gcatgtcggt cgagcaaatg gcgcgcattt
     6541 ttgagccttt ctacaccact cgtcgggcca gtggtggctc cggactcggt ctgtatattt
     6601 gctacaacat tgtcaccgtg caactgcgtg gcacgattgt gtgtgacagc cagcccaacg
     6661 ttggcagcac attcaccatc aaatttgcgc aggagatcct gcccttgcag gaggaaaacc
     6721 agccatgaaa ctcatacgta cccgcagcga gcccgctgcc agcgccgacc atcccgctgg
     6781 cccgcaccac cactggaagc tgctggtggt ggatgatgaa cctgacatgc gtgaggtgac
     6841 gcggctgaac ctcaatggtt ttcgctttgc cgaccgcgac ctgaacattc tggaggccag
     6901 ttccgcctac gaagcccgcg agttgctggc cgcgcacaag gacattgcga tggcgctgat
     6961 cgacgtggta atggaaaccg acgatgccgg cttgaggctg gtggagtaca tccgtaaaga
     7021 cctcaagaac ctgatgatcc gcctgatcat tcgcaccggg cagcccggtc tggcacccga
     7081 gcgatttgtc attgaccact atgacatcga cgactacaag gacaagaccg aacttaccgc
     7141 cagccgcctc tacacggcgg tgcgcgcggc actcaagtcg ttccgcgatc tgcgcaccat
     7201 cgacctcaac cgccagggtc ttgcgcgggt gctagaggcc gcgcccgaca tttaccgcat
     7261 caccaaccgt tcactgcaac agttttttca gggtgtcctg acacagatca tcgggctttg
     7321 caatctttcg gattcaagtt ttatttccgc cgtcggcggc atcattgcca cctttgatga
     7381 gcgcgatgtc acggtgcagg caagttcggg caatctggcc gcccaggagc gctttgagaa
     7441 catccgcgcc cagtgttcag aggcagttct gaccgggcaa atgcccgcag gcctgcgcaa
     7501 ggacgcttac gtggtgcccc tgctggtgca gcgcaagccg gccggttttg tctatatcga
     7561 gccgacccag gagctcagcg atgccgaccg tggactgatc aacatggtgg cccagcaatg
     7621 ctccaacgca ctggaaaacc tgcgcctgca cattgacctg gcccggtcac atgaccatat
     7681 ggctgacatg ctggccaagg tggccgagtt caaggaccac accaccggca gccacatccg
     7741 gcgcattgac cactacacgc gtctggtggc gatcgagttg ggcatctcgc catttgaatc
     7801 cagcctttac ggcaaagcca gtcgcctgca cgatgtcggc aaagttggca ttcccgatgc
     7861 cattctgggc aaacccggca aacttgatgc ggcagagttc gacctgatgc gcacgcacac
     7921 ccacatcggt ggcattatcc tggaacgtga ccggtttctg ggcgtggcct gcgacgtagc
     7981 gcgtcaccac catgagcgat gggacggcaa aggttaccct gacagccgcc cctcacgcga
     8041 gttccatctg gtgacccgca tcgtcagcgt ggtcgatgtg tttgacgcgc tggtcagccc
     8101 ccgcccttac aaggagccct ggccagcgga gcgcgctgcc cgtgaaattg aagccggtgc
     8161 gggcacacag ttcgacccga cggtggtggt tgctttcctc aaactctttc ggcaaggcaa
     8221 attcgaccat atcgtcggcg cctcacagca ggccaccgac ctgcccgatc cgttagacga
     8281 gatgtaagct ggtccacggc aggtcaactg ccagcgcaac gcagactatg atgcagggcc
     8341 aattgaagcg agatccaaaa atcctatgaa aatgactcag aaagccacct tgatgctggc
     8401 caccgccagt ttgatttgcc tgcccgctgc ctgggctgcc gatgccactt tggacgcggc
     8461 cgccaaggaa cccggtgcca tagtgaccgc cagtgggctg gtctatcttt caaccaaaaa
     8521 tggcaccggt gacagcccca aggccagcga caaggtcaag gtcaattacc gtggcacctt
     8581 tcccgacggc cgtgaatttg acagctctta caagcgcaac caggcgattg agttcccttt
     8641 aagcggtgtc atcaaatgct ggaccgaagg ggtaggcctc atgaaagtgg gtggcaaggc
     8701 caaattgacc tgcccggcat cgattgccta cggcgagcgt ggtgccggtg gcgtgattcc
     8761 gcccaacgcc acactgcttt ttgaggtcga gttgctgggg atcaacggca aataagccgc
     8821 gtgcagcgac agatcacgcg tcgcaggaat attcacccaa agccgttaga caggctttga
     8881 catcaggctt cctgatttgc gctgcgaaac cggtcggcat ggctgtgccc agcgcaggcc
     8941 agatgccggg tcacgggtca gccacccagc gcttcggttt tggcgatggt gaccagggcc
     9001 tcatgccatt tccatatcaa tgcgccatgt tgttgctttg ccgtgccgtg ttgcggcact
     9061 gtctgcggct ttgcgcccaa tgtcgcatcg atctggaaac gcaatcagcc agttcctgca
     9121 tcacgccttt gggccaaggc aatatgcttg tcggcgcagt agggtcatgc aggtccgcag
     9181 ccacccaggt cggccagtat gcgtcggcgt ccatcctgct gcagtctgcg ccaatttggg
     9241 cgctgtgtgg cctgcggcgg ctggcacagc atggcgggcc gctgcgccat tgttgacatc
     9301 gcttcttgaa cgcccttttt tgcacttgcc tgcagattgg catcctgcag tgcctgggct
     9361 accagtgcgc ggtcggcctc atgaaacagc agcttgcgcc ctcccgcaaa gcggttgcga
     9421 agcgcttggt cataggcctt ccaatgcacc gccagtgccg gggtgggtgg tgccagcaga
     9481 tgcgcccaga ccagcacttc cagtacctgt tgttgcggcc cggtccagct tacggtgacc
     9541 ttggccggca ggttctgcag cgccagtgcg gccatctgca ttgcgtctgt tgcgcgccca
     9601 tggtcgatca gaaaccaggc cagatcaatg cgcgacaaga cacccgcgag cgcattttgc
     9661 aagcctttgc gcgttcggtc tccagcatcg ggcactggca ggctgtcgat acgtgtctgc
     9721 aagacggctt ctgcaccagc gctgtcggca tttgcgtcca gggcattggc cagggcaagc
     9781 cgggtgtagg acaagctgtg gtcaagagcc ggcatggcag cggccctcag ggccactgca
     9841 tcacgcaaca atgccacccg ctcgggtgct ggcaagtcgc cgtaggacaa ctgctctttg
     9901 gcagaggcca cgcggacacg ctcggcaccc tgaggctgga ccagttccgc aacaatacgc
     9961 gacaggttgt catgctgctc cagatcggag tcggtcgcag ccgccagccc ttcgagtgcg
    10021 tcgatgcggc gcaggtcgcg cagcggcaga tctttggcaa tttcccaggc caatcggaaa
    10081 tggcgctctg cggtgtcccg gtcttcaacg ccattggcct cctggcctgc gcccagatag
    10141 gcctgtaaca ggtcttccct ggacaagttt gcagcctgcg ccagccgggc gttccagttg
    10201 acaggttgtg ccgctggcgg gtcggcgggc tcggggtcca catcatcgcc tgccatggtc
    10261 aagccagatg ccatgtacgg cagcactgcg cccaactggg gcactgccac cagggctgct
    10321 gcaatcgggc cgatcaaaca aacagcatag atcaacaggc cgacaaaggc ctcgctgcgc
    10381 cgcggtcgtc gccccatcaa ctccggcagt agcgctgtgg caacggcagt tcgcctggca
    10441 aaggaccagg cccgaaacgg tgccatctgc aacgcggcaa atatccttcg cagagcctgc
    10501 ggctcatcga ccccatttgc gtcgtcgcgc gctacagcca ggtcaaggcg catcagatgc
    10561 cactggtgcg acaagccaat ggccagtaac acggcgaaca ctttcagcac caggtcatcc
    10621 agtgcaatgc cacctgccag caaaaggcaa caacacagca cggcaaatcc gaagcgccag
    10681 cgggggtggc gtgcaaacgc aaatgtctcg agcacacgcc caccgtcgag tggcgtgatg
    10741 ggaagcaggt tcaggtagtt gatggccagt gacgcgatca gaaactcgtt cagccagccc
    10801 ggtgcaagcc aatgcccggc cgaactcgcc cagaacgctg cacccgccat gacaatgccg
    10861 ggtaccggcc cggccagata gacaaacagc ttttccatgg gcgtggcagt ggccttttct
    10921 cctgtggcca ggccacctag tccgggcaag aaaaacaccg acacattgcg atagccggtg
    10981 agcctcatcg ccaggaaatg tccgccctcg tggagcgcaa tcacggccag caacaccggc
    11041 acaaagctcc aggaaaacca ccagccaccc accaccagaa acaacgcggc gcttaccaca
    11101 aacatcaaaa gcttggctgt gccgccggac gtggcggcgc ggcgcaaggc aagctgttcc
    11161 tcaaatgctt gcaggtcagc ggccagaccg tctgctgaac cggcataccc agtggctggc
    11221 ggcagaccgc gggtggccca cgcagcgcgc cattggccaa ccagcgcgat ggcagcaaaa
    11281 cgcagggcag ccagccaggt caggcggtag tgcttgctgt tggcgatagg caccagttgc
    11341 ccctgccgaa gcatctgggg aatcagttct tcaaaggtct ggtccagacg gcgcttgatt
    11401 tcgatgccgt cggtacacag gtccggcaag ggcagcatct gcacccgctg ccggtgcgcc
    11461 tgccatgcct gggcccactg cggcaggtaa tcgtcaaaca cctgccagtt cggcatggtc
    11521 atgggtgaat agtgccgaca gcggttcagt gtggtccagt tactgccatc ggccagactg
    11581 gtcaccaact ggatcatgca cggctggcgc gggtcaggca ccggtgccag tgacaccatg
    11641 gcgtgggtgt ggccatcaat gtgctggtac acatcaaaga acaggggctc gtccttggtg
    11701 ctgaccaatg cccttctggc agcaccgctg tagcggtaaa caaaacccag ctcagccaat
    11761 tgctgggccg gcaaatcaag aatcgtacgg ctgctggccg gcacgtcttc gcataccacc
    11821 gagtggcaac gcgtgcgctg ccaacgcaga cgcgccagac gctgcacgaa gatcaccagc
    11881 atcagggcga tcaggcagca tgccgccaga agcacgacca gcacagcttg ttgcaacatt
    11941 cccataggtc accagtagtt gtccgatggg gcccaatgat gccacgccgc aaaatcagtc
    12001 agcccttatt ggactgagcg ggctacggac gcaagacaaa attgaacatg gccaagaacc
    12061 aatgcgatcc aaaagcaggc atgaaacgac acaacgaaag tgctgggcac cagttggacc
    12121 tatgccctcg gttgcgaacc gcacaggggc cgatcttaca gcggcttttt ttctcacagt
    12181 tgctcccggc tgcagtgtcc atcaactggt tgattggtga acgggcacac gctgcagtgc
    12241 agattcgcta cgctgtggcc cagtggcagg cgcatccagc atcgccgaaa ttgcgcgact
    12301 gaaccgggct gtttttttgc gcagcgaagc agccttggcg tgacctgcag ggtgcagttg
    12361 catggctctc cagcggttca atgactgcgc cacggtgcga tacttggtct tgcgtgcctg
    12421 aaaagccggt ccgtttaccc ccattttgta acgaacaatg acggaccggg tgttcttgcc
    12481 cacgcacaat ttctgtcgtt gaccaagttg ccctgttgcg gcagatgcca ttcttgattc
    12541 ctggcagagc cggaaatccg ggattcctcg gttgaggcga tgccgaattc aaaaaagcct
    12601 tatggccggg tcggctttca agacgacttt tttaacgcgg cacaattgct ttttcgggcg
    12661 atctcggcac ttggtcaagt gctcccgcgg agcaatgaca tggcggttta tatttcaact
    12721 tgaactggag aattttggat gattcatcga cttgatgcgc tgacttttgc atgcctgatg
    12781 gcacttttgg gttgcggtgg cggaaccgag tctgccgcaa gtccgccggt cgttgaagtt
    12841 ccggtcttga atgcttcgtt tgatcaggtc tctgccaccg gggcgctggt ttcctggttg
    12901 gcaaccgaac acagcaatgg ccagtcctac acctttcaag ccgattccaa gggtgcctgg
    12961 tcggcaccga gcagcgcaaa aatacaccgc tatggattag aaaccttcgg cctgttgaat
    13021 cagcacatcc agcttcagcc tgcctggatc aacaagaccg cacgactgtc ggcgcagctt
    13081 aagaccgaag gcgcgactga tggcggtggt gccctggtgc tgcagatgat cggcagccgt
    13141 ggcgatatcc tggcctggaa ccacatggtc gatgcgagag ccaagcagac gcaagactgg
    13201 aagcaataca gcattgacct gaaggtcttg tcaaccgcct tgaccatcaa cgtcggtgtc
    13261 atgctcgaag gcgctggcac cctgtgggcg gacgacatca agctggaaat cattgattga
    13321 agcaacctcc atttctgatg ccaaatcaat tgttgacctg tggtttcagc gcatcggcca
    13381 gcggtttttg acgctgcgac gctgaccctg tcctgatggc cccggatgcc tgccactccg
    13441 aatggcgata cagcaacatt caggcgcttc gttcgagctt gccccaccgc atggtgccat
    13501 ctttggcgct agcgcccgtc caccttgcat agactgctat caaatttgtt aactcccctg
    13561 aagcaactga tccgccattc gagccagcgg cagcgttttc tggccgaagt gcgcgcactg
    13621 tggctgctgg cctggccgat tctggttggt caactggcca ccgtcggcat ggccgtggtg
    13681 gatgtcgcca tggctggtca cgcttcggcg caggatctgg ccggagtgtc cctgggcgtg
    13741 tcgatctgga acatgctgat catcaccctg atgggcctga tgatgtcggt caacccgatg
    13801 gtggcgcacc aggttggcgc gggcgacctc agtggcgtgc cgcatgtggt gcgtcaggct
    13861 ctttggcaag cgctcgggct ggggctgctg gccggcctgc tggccaactg ggcggcgctg
    13921 ctgtttgacc acctgacgct ggagcccccg gtgcgggtgc tggcgatcaa gtttgtcttt
    13981 gtcaccagcc tggcgttgcc gctgtttgcc tgctaccggg tgctgtacgg ctacagcacc
    14041 agcatcaatc agaccaagcc gctgatggtg attgcgttgc tgtcgttgtt gctcaatatt
    14101 gtggtcaaca gcctgctggt gtttggcgcg ctcggttttc cccggctcgg cgggcttggt
    14161 tgtgcctggg ccacgctgat cacggttgcc ttcaatttat tcgccttgct atggtggatg
    14221 caccagtcaa gtgcttaccg cagcacctgg ccatttggtc acttcgagcc gccgcagtgg
    14281 cggcaaatca gcagcttgct gaaactgggc ctgccaatcg gcatcaccta ttttgccgaa
    14341 accagtgctt tcagcctgat tgcgctgctg gtggccgaat ttggcagcac gcaaatggca
    14401 gcgcaccaga ttgcactcaa tttcacctcg ctggtgttca tggtgccatt gagcctgggg
    14461 ttggcgctgc tgactcgggt cggtcagtcg ctgggggctg gcgatgcggt gcaggcgcgc
    14521 taccaggcgt gggtcggggt ggcactgggg ctgggctttg cagccttgtc agcgctgttg
    14581 atcgcctctt tcagcaccgc cattgccagc gcctacacca gtgatgcagc ggtcatcggg
    14641 ctggcggcgc aactgctgtt tctggcggcg ctgttccagt tgtccgatgc cacccaggtg
    14701 gtcaccagtt gcgccattcg ggcctacaag gtgacccgct cgccgatggt gattcacttg
    14761 acagcctttt gggttttttc gctgccgctg ggctacgcgc tgggcattgc gccaaattgg
    14821 ctggcctggg caccggcgcg accgatggcg gcgcagggct tctggattgc gctggtggtg
    14881 ggtttgacaa ttgcggcact cgggctggtc tggctgttgc gccgggtggc gcgctcgcgc
    14941 ctgccagcgt gatgccagtg ccagcagccg acgtttgcgg tctgattgac cgcaccgcca
    15001 ggccgccaag caaagccacg accgtcacac ggcaaccgca catcgatcaa aaagttggcc
    15061 gccatgattg ccaaaacatt gaacaaaata gccctgtagc ccccgtccca catgcgctgg
    15121 cagctattga ataagtagcg tgcatgcgcc ggaatgcagg cgcgacagct tgcagggcag
    15181 gcggcacgaa ggcatcgtat tcggtgacga tgcaaaaatt tgaacagtac ggtaacctgc
    15241 gccaagtgat tcgcacccag ccactggcaa taccctgccg acccgttgtc accgcgcccg
    15301 ccaggtcgcg accagctttt caaacgcctg ctgtggcaca ggccggctgt acaaatagcc
    15361 ttgcagcagg tcgcagcggt gcaccgtgct gagaaaatag cgctgggcat tgtcctcgac
    15421 accttctgcc accaccttca agcgcaagct gtgtgccagg ctgatggtgg cggcacaaat
    15481 gaccatgttg ttgtcgccca actcaatgtt ggtcacaaag gaccggtcaa ttttcaactg
    15541 gtccagcggc aagcggctca ggtacgccag cgacgagtag ccggtgccaa agtcgtccag
    15601 cgaaaagccg atgccaagcg ccttgagctt gaccatcatc gccacgattt catcaatatt
    15661 gccggcaaaa acactctcgg tcagctccag cttgagttgc tgcggggcgg ctccggtgcg
    15721 cgtcagcgtg gcgatcacct gctcggcaaa atccggcagt ttgaactgct gggcgctcac
    15781 attgaccgcc accgtcagat gcgccatgtc tggcctgcca gcccaggccg ccaactgctg
    15841 gcatgcggtt tccagcaccc actggcccag tggcaggatc aggccggtgc gctcggcatg
    15901 ggcaataaag tcgttgggtg gcaccagccc gcgctgcgga tggttccagc gaaccagcgc
    15961 ctcggcccct tcgatctggc cgctgctgcc cgacacctgg ggctggtaat agagttccaa
    16021 ctgccccgcg actatggcct ggcgcagttc gctttccagg cgcgcatgct ccagcgcgga
    16081 agactccatg accgggtcaa aaaacacaaa ggtgttgccc cccaggcttt tggcctggta
    16141 catggccagg tcagcctgcc gcagcacgtc gtccaccgag gtctcctgcc ccctgaacag
    16201 ggtaatgccg atactggcac tgcaatggaa gtcggcattg agcagttcat agggctgagc
    16261 cagcgctgca agcagcttct gacaggcaag ctcaacctcg atggcggcag cagccagcgt
    16321 gctggcctcc acgccagaca acatcaccat gaactcgtcg ccgcccagcc gtgccaccgt
    16381 gtcgcccggg cgcaagttgt cgctcagacg ctgcgctacc tggatgagca ggacatcgcc
    16441 gctggcatgg ccacgcgtgt cgttgagttc cttgaagttg tccagatcaa ggaacagcag
    16501 cgcgccatag gcacccacac gcgcattggc cgccgtggtc tgctgcagtc gctccagcag
    16561 caagatgcga ttgggcaggc cggtgagctg gtcaaaaaac gccagttttc tgattttttc
    16621 ttcagccagt ttgcgctccg agatgtcaat ataggcaccg atgtagtgcg tgaccgctcc
    16681 gtgccggtct ttcactgctg agatcgacaa ccatttcgga aacacctcgc cgtttttt
//
LOCUS       DAOSLL010000017        16658 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_3645_length_16658_cov_4.504367, whole genome shotgun sequence.
ACCESSION   DAOSLL010000017 DAOSLL010000000
VERSION     DAOSLL010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 16658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16658
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3645_length_16658_cov_4.504367"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="PLB25_01370"
     CDS             complement(<1..727)
                     /locus_tag="PLB25_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HOE40275.1"
                     /translation="MTTSNWISRKVSKLMPGKTVRKNATEKAVRTVQATVEPSSVTEL
                     NSRPAGAQPVAPGLADTPASSGTRRTPRSTTERLKATFRQDDEALSPRELRHKLSELL
                     AVVDIQVSEVEGGRRARLLAQWYAAAKPEQRHDLWLLMSEQFVADPQVIQQAQAELDA
                     AQGTQDQAVAEVNFRRATVSRRRRLLQRFSAFAEGIRFLVDLRAEMLPALKSDKRLQA
                     LDVEMEYMFSTWFDVGFLDLRRIS"
     gene            complement(826..1098)
                     /locus_tag="PLB25_01375"
     CDS             complement(826..1098)
                     /locus_tag="PLB25_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40276.1"
                     /translation="MLVQVAAQHDHHRCVAAAGNHFDCGVFGRLRLVLSLYHTGLGDG
                     LFEFFAMGVEDQSGFAQFQVDFHREIDVFSQALNKNCALSKATIDH"
     gene            1111..1485
                     /locus_tag="PLB25_01380"
     CDS             1111..1485
                     /locus_tag="PLB25_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40277.1"
                     /translation="MLVRQLLVSSGEIEPSDATTTLSLCIKIHRIATPAHDKAIAALL
                     ADLSSEELRHPETGTKKIYTLASKYISDADRKITSVVGDFCSSLQSAVAAFFGNELKS
                     VPLGPGSNQFGHPRTSSERGTA"
     gene            complement(1567..1809)
                     /locus_tag="PLB25_01385"
     CDS             complement(1567..1809)
                     /locus_tag="PLB25_01385"
                     /inference="COORDINATES: protein motif:HMM:NF018894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOE40278.1"
                     /translation="MPIEFKGVAGLTKNISATGIYFESQVDQAPGSVVQFVVDVEVQG
                     ELFKMICKGEVVRVEQKDGVVGVAVKLAHSFFTDPQ"
     gene            complement(2233..2925)
                     /locus_tag="PLB25_01390"
     CDS             complement(2233..2925)
                     /locus_tag="PLB25_01390"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOE40279.1"
                     /translation="MPIDDRARLRIRLSGLLFVAASLIALALAWNWSPLRTWLDIDLM
                     VASLQRLGQTFGIAAAVSGFALAVSLAVPLTFLTLVTLVALGPWVGFACSMAGAIISA
                     ALSYGLGRYLGREVVQHLAGQRVNLLSQRLARHGLLAVVVMRLVPVAPFAVVNMVAGA
                     SHIRLRDMLLGTCIGMMPSTLLMAIFMHQIITALKQPNTTSALLLALTAVLVLVGVLG
                     VRHWLRHANVTE"
     gene            complement(2915..6769)
                     /locus_tag="PLB25_01395"
     CDS             complement(2915..6769)
                     /locus_tag="PLB25_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HOE40280.1"
                     /translation="METTTVSSLGARQVSLSLSQREVWLDQCAWPDSAHLNIGGGAFL
                     RGRFDLPRFKAALSCLVAENEALRLAPRLDGTQALLAQFEPTLEWVDLSGAANPEATM
                     RVWWHKRIQQAFVLNGQPPWRFALLHASDELHGLTIQFHHLVMDGWGTAQVMQRWSQI
                     YNALDAGVQPESTNCPDYLQFIDESNEYRDSQAFERDAAYWRSQMPTLPEPLVVRRYA
                     PLRPEHLPRSRLAKQKIPRSDYAHLEAYASTQGTTAFNFFLAALALYFSRINGFQEVV
                     LGIPSLNRSGRRFRSTPGMFVGVLALKVVVTESLTVADLLAATGSALRGALRHPRYPL
                     SELGKALQLIRTGRDGLFDVLLSFERQDYSAAFGSARLVDSRQIFSGTARYPLGVTVC
                     EFHPDQDIELVLDASSDCFATGEPALLGRRLWHLAQALMAEPSTPLEAISLLPPEEEW
                     ALLQGLHKAVASHEATQTYISQFEHQAALRPEATAVVWNGGNMDYATLDQKAEQLSRR
                     LKASGTAAGAIMAVAIARSPNMMVALLGIAKAGAAFLPLDPEAPVARIAQILQDSAAS
                     ALLIEKADAARFANLHGNTLVFDSNSPPTPEVWLDTLPAWIEPQPDDLAYVLFTSGST
                     GHPKGVMVTHATLSRRLAWLSRTYAVDWHDRSAQATQLTFDPSLIELFLPLIHGGSVA
                     IPPPGRLLPESLADFAIGHGVTIMAFVPSTLSRFLDGAVGKTGLKLRVACCGGEVLPP
                     ELGNRFITETGARLYNVYRPTEAAIFASAWQCELHPVDVALPVGRPIDDTRIYVMNAQ
                     LQLMPLGVTGEVFIGGAALARGYLNRPELDAQVFLNDPFQPGARIYRTGDLGWLGLEG
                     NLHFCGRIDRQIKLRGYRIELGEIEATCLALPGVLQAAAKLVADNGKPAIHVWVATNP
                     GVNSHSLQQALRSRLPDYMIPAGIRVLDKLPQTSAGKVDFAALPEISRVPLVSGVRLP
                     ASALERDLMALWQAVLNPPTIGLADNFFDLGGDSLAAVSILTGIEKLLGRRVPMHLLI
                     EHPTIEQLALALKSDVMAPSVLIELGSRSGQVPLYLAASGHGDLLRFQNLAHALGSAC
                     DVQLLQPPTDKAVATVAELAGLYATSIANQRLAPGFIAGFSVGGVAALETARLLRQQG
                     VAIRGLVLLDTIYPKGALGGAMSWRILGWLVRHLHVQELSMNGRRLGAMFNDPGLISQ
                     VMALRGYQAASFDGPAWLIKSSGLVSWDRWFFRPWRRLFTAQLQELQVPGLHGSIFEA
                     SNVNALAGVITQVLQTRHDDAH"
     gene            complement(6727..8535)
                     /locus_tag="PLB25_01400"
     CDS             complement(6727..8535)
                     /locus_tag="PLB25_01400"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOE40281.1"
                     /translation="MNLRERAYKVLRAGRAFGSLEEPVLQDLAELLEFQSVRGGTTVQ
                     HEGEPSIDMLFVLHGGLRVSRRTVAGQVNLYNEIRPGQSVGEAGLILQQPRTADVTAM
                     RDSILAVLSRANFEALLIRYPLALSRVFVQSNLPFARDSRPVVEQRYAQTYVVIGLHE
                     GADVGTVAADLKKAFAQRGRVHHFKPPQGGSSEPTSMNAEWDNQLDEEYDFLVYEAEP
                     GLSVWTQRAFRQADQVVFVASVGASQALGVIEQGLTQELGFPMKRKHLVLLHPATALE
                     PTEVARWRDTARYERIYPLRTQQESDFSRLARFLTGSAVGVVLGGGGARGFAHVGVLR
                     AMEEAGIPIDLVGGNSMGALIGAQYVSGVSLDAILSQTQHFAGGGEHPTLPLVSLVSG
                     RRVERDLKRMFGDTMIEGLWRPYFAAACNLSRGKTCVQETGPLWRAVLASNSPAGLFP
                     PVLDNGELLVDGAILDNVPVSAMRVRLGTALEKRHGNGTIIAVDVDVPDALGADPTLA
                     RLSPWQVIKGSLFPGTHPSPNIADILYSVGHVGSVSQRGRTMAQADHFLEPPVAGFSL
                     MGYRHAHAIAEVGYRYAQGKIAQWKQQQSARWEPGK"
     gene            9698..10582
                     /locus_tag="PLB25_01405"
     CDS             9698..10582
                     /locus_tag="PLB25_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA4780 family RIO1-like protein kinase"
                     /protein_id="HOE40282.1"
                     /translation="MKAPPRLQPLIEEGLIDSVVRQLMSGKEATVFVVRCGDETRCAK
                     IYKEATNRSFRQAVDYTENRKVKNTRQARAMAKGSRFGRQAQEAAWQSAEVDALYRLA
                     AAGVRVPQPYNFHEGVLLMELVTDAQGDAAPRMNDVAFTPVQALAHHGTLMAEVVRML
                     CAGVVHGDLSEFNILLAYTPGDGDDAGTDIPVIIDLPQAVDAAGNNHAQRMLLRDVAN
                     LRDFFGQFAPELLATHYGPEIWHLYQAGLLTQDSVLTGHFEQAQGNVDVGGVMREIDD
                     ARAENAARKLRMQTATAP"
     gene            10934..12313
                     /gene="hisD"
                     /locus_tag="PLB25_01410"
     CDS             10934..12313
                     /gene="hisD"
                     /locus_tag="PLB25_01410"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HOE40283.1"
                     /translation="MNLIATPARLSTTGSTFDADFKARLHWSAQTDAAIEQRVADILV
                     DVQQRGDAAVLDYTARFDGLQATSMAELELTQAELQLAFDGLPELQRLALVAAADRVR
                     SFHQAQRKASGESWSYRDADGTLLGQKVTPLDRVGIYVPGGKAAYPSSVLMNAIPAHV
                     AGVAEIIMVVPTPVRGSVASGGQGGALDAKPELNRLVLAAAFVAGVTRAFTVGGAQAV
                     AALAYGTATIPKVDKITGPGNAYVASAKKRVYGTVGIDMIAGPSEILVLADGSTPPDW
                     VAMDLFSQAEHDELAQSILLCPDAAYIDLVQASINRLLPEMPRRDIIAKSLNGRGALI
                     HTRSMEEACDISNRIAPEHLEVSSRDPQRWEPLLKHAGAIFLGAYTSESLGDYCAGPN
                     HVLPTSGTARFSSPLGVYDFQKRSSLIEVSEAGAQRLGQIASVLAHGEGLQAHARAAE
                     MRLFARNRG"
     gene            complement(12380..13369)
                     /locus_tag="PLB25_01415"
     CDS             complement(12380..13369)
                     /locus_tag="PLB25_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40284.1"
                     /translation="MRRREVSGLGTILAALAVVPMAPAMAATWNQPEYTIQRALYGTP
                     ERNVDVTQRLRELAANNERFILSNETFGVDPDKGRTKTLRIIATDPSGRPRTFEYTES
                     SWVDGAQFSGWSGGNWEQGNGQQGQGGWGNRPGYHDGGNGGYKILQARYGTADRHVDV
                     TQRLRELARRNERFLLSNETFGVDPDHGRTKTLRIHARGSNGQMRTFEYTESSWVDGS
                     HFSGWGSGNWGQGGDGNWGGDRPAVGGGNYDNYDQGLRILRAEYGAGRDRLDIAERLQ
                     QRVYNGRLNIKVDNNMAGGDPAEKRPKQLWITYSVNGRERSRVVPEGERLSIP"
     gene            13637..14746
                     /gene="hisC"
                     /locus_tag="PLB25_01420"
     CDS             13637..14746
                     /gene="hisC"
                     /locus_tag="PLB25_01420"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOE40285.1"
                     /translation="MTLLQSIIDSRIRSDVQAMHAYVVQDATGMVKLDAMENPHRLSP
                     HLQAKLGQRLGALALNRYPADRLNDLRAALACHAHMPAGFDIMLGNGSDELISLLALA
                     CAVPPSTANGFQKACVLAPLPGFVMYAMSAQLQGLDFVGVPLTASFELDLPAMLNAIA
                     QHRPALTYLAYPNNPTANLWHDAAMQSVIEAVAEQGGLVVLDEAYQPFASASYIDRLS
                     RHSHVLLLRTLSKFGLAGIRLGYLIGPRPLIAQIDKVRPPYNVSVLNCEAALFALEHQ
                     EVFQAQALDVIAQRAMLFEALGRLPEVRAFPSQANMMLLRVPDADMTFEGLKARGVLV
                     KNVSKMHPLLNQCLRLTVGTEPENRQFLAALEASL"
     gene            14743..15381
                     /gene="hisB"
                     /locus_tag="PLB25_01425"
     CDS             14743..15381
                     /gene="hisB"
                     /locus_tag="PLB25_01425"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HOE40286.1"
                     /translation="MTNYPLTELPEASTSSAPRKAELTRNTAETQISVRVNLDGTGVS
                     RLHTGIGFFDHMLDQIARHGLIDLDIDAEGDLHIDAHHTVEDVGITLGQVVNQAVGDK
                     KGLRRYGHAYVPLDEALSRVVIDFSGRPGLLMNVPFTSGSIGTFDTQLVHEFFQGFVN
                     HAFVTLHIDNLKGDNAHHQVESVFKAFARALRSAVELDPRALGQIPSTKGSL"
     gene            15433..16137
                     /gene="hisH"
                     /locus_tag="PLB25_01430"
     CDS             15433..16137
                     /gene="hisH"
                     /locus_tag="PLB25_01430"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HOE40287.1"
                     /translation="MISIVSSSAKKTVAVVDYGMGNLRSVTNAVMAAARGIPVEVVWT
                     QRPEEVMAADRVVLPGQGAIRDCMRELRDSGMYPAVMHALINKPVMGVCVGMQMLLDH
                     SHEGYAGDAGQGTPCLGQIHGEVIKFELAGQLQPDGSRYKVPQMGWNQVYQNSHGATP
                     HPIWDGVPDGCYFYFVHSFYAKPSDARHSVGETDYGQRFSSAIARDNIFATQFHPEKS
                     AEHGLSLYRNFLHWNP"
     gene            16308..>16658
                     /locus_tag="PLB25_01435"
     CDS             16308..>16658
                     /locus_tag="PLB25_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HOE40288.1"
                     /translation="MLLIPAIDLKDGHCVRLQQGDMKLATVFSEEPVVMARSWVDKGA
                     RRLHLVDLNGAFAGHPKNEAAIRKILQDVGSEVDVQLGGGIRDLDTIERYLDAGLRYV
                     IIGTAAVRNPGFLQD"
BASE COUNT         3606 a         5020 c         4702 g         3330 t
ORIGIN      
        1 atgagatgcg gcgcaggtcc agaaaaccca cgtcaaacca ggttgaaaac atgtactcca
       61 tctcgacatc gagcgcttgc aagcgtttgt cggacttgag cgcgggcagc atctcggcac
      121 gcagatcgac cagaaaacgg atcccttcgg caaaggcgct gaaacgttgt agcaaacgcc
      181 gccgccgcga taccgtggcg cgacggaaat taacctccgc tacagcttgg tcttgcgtgc
      241 cctgagcggc atcaagctcg gcctgcgcct gctggatgac ttgcgggtcg gccacaaact
      301 gctcgctcat caagagccac aaatcatgcc gttgttcggg cttggccgca gcgtaccact
      361 gagccagcaa ccgggcgcgg cgaccacctt cgacttcact gacctggatg tcaaccaccg
      421 ccagcagttc agacagcttg tggcgcagtt cgcgcggcga caaggcctcg tcatcctgac
      481 gaaaggttgc cttgagacgt tcagtggtcg agcgtggggt gcgacgcgtg ccgctgctcg
      541 cgggcgtgtc ggcgaggcct ggcgcgacgg gctgcgcccc ggctggccga gagttcaact
      601 cggtcactga cgatggttca acagtggcct gcacagtccg aacggccttt tcagtcgcat
      661 ttttgcgaac ggttttgccg ggcatcagtt tgctgacctt gcggctgatc caattggaag
      721 tagtcatttc agttacctat ctgaattatt gctgtcaaat cattgtttca cgattgtgat
      781 tggacttcct gaacgagaac tcaaaacctc gcacaccccc tgatttcagt ggtctatcgt
      841 cgcttttgat aatgcgcagt ttttattcag tgcctgactg aaaacgtcaa tctccctgtg
      901 gaaatccact tgaaattgag caaagccact ttggtcctca acccccatcg cgaagaactc
      961 gaaaagacca tcgccaagac ccgtgtgata caaagacagc accaatcgaa gtcggccaaa
     1021 gacaccgcag tcaaaatgat tgcctgccgc agcgacacag cgatggtggt catgctgcgc
     1081 cgccacttga acaagcaaga ggaggcctga atgctggtgc gtcagttgct ggtctcaagt
     1141 ggcgaaattg aacccagcga tgcgactacg accctgagcc tatgcatcaa aatccacaga
     1201 atagcaacgc ctgcacatga caaagccatc gccgcactgc tggcagacct gtcctctgaa
     1261 gaattgcgcc acccggaaac cggtaccaag aagatctaca cactggcatc aaagtacatc
     1321 tccgatgccg accggaagat tacctctgta gtcggtgact tttgctctag tttacagagc
     1381 gctgttgctg cattttttgg caatgagctt aagtcagtgc cgttggggcc tggttcgaat
     1441 caattcgggc acccccgaac ttcatctgaa agggggaccg cctaaaccaa ctgaaaattt
     1501 tcgtgacagt tggtgccact ttagattgcc ctaacccccg aaggaatcac caactccgaa
     1561 gacgactcat tgaggatcag taaaaaatga atgggccagc ttgacagcga cgccaacgac
     1621 accgtctttc tgctccacac gaacaacctc gcctttgcag atcatcttga acagctcgcc
     1681 ctgaacctcc acgtcaacga caaactggac aactgaaccc ggtgcctggt cgacttggga
     1741 ctcaaaataa atgcccgtcg cactgatgtt cttggttaat ccagcaacgc ctttgaactc
     1801 aatcggcaac tcagcattga acctgtcagc aagccgctgg gtcttggatt ggccactctt
     1861 tccagttgaa ggtatgttgt gcatctattt ttataactac tcacgtatta tgaatcaacg
     1921 gcttacatct gatgttcgtc agaaagtact caagaagcag gcaaaattcg gaaaaatcga
     1981 gtcaaaattt ctctttccaa gcatgaggaa aatcatcagt tattgattca attcaatcat
     2041 cgccactcaa ggggtgctga gtagttacct attttttgat cagcaactgt tcaaacttgg
     2101 tcatcaactg cagctaagca ataacaatgc catttacatt tttttcattg taagcaaatt
     2161 gaatcaataa cttacgcaag gattgcgagc acttttgaac caaatgccgt agccctcatg
     2221 tctgactgta agttattccg tcacattggc atgacgtaac cagtgccgca cgccaagaac
     2281 accaacaagc accagtaccg cagtcagggc cagaagcagc gcgctggtgg tgttgggttg
     2341 cttcaaggca gtgatgatct gatgcatgaa aattgccatc agcagcgtgc tgggcatcat
     2401 gccaatacaa gtgcccagca gcatatcgcg caaacggata tgcgaggcac ctgcaaccat
     2461 gttgaccaca gcaaacggcg caactggcac caatcgcata actacaacgg ccaaaagacc
     2521 atgccttgcc aggcgttggc tgagcaaatt cacccgttga cccgcgaggt gctgcaccac
     2581 ctctcgtccc aaataacggc ccaagccata actcaatgcc gcactgatga tggcacctgc
     2641 catggaacag gcaaaaccga cccagggtcc caatgcaacc agcgtgacaa gtgtcaaaaa
     2701 agtcagcgga accgccagcg acacagccag ggcaaaacca ctcaccgctg cggcgatgcc
     2761 aaaagtttgt cccagacgct gcaggcttgc caccatcaga tcaatgtcca gccaggttcg
     2821 cagcggcgac cagttccagg caagcgccaa ggcaatcagg gatgccgcga caaaaagcaa
     2881 acctgacagg cgaatcctca gtcgggcgcg atcatcaatg ggcatcgtca tgtctggttt
     2941 ggagcacttg agtgatcaca ccagcaaggg cgttgacatt gctggcctca aaaatggaac
     3001 catgcaaacc aggcacttgc aactcttgca gttgtgctgt gaacaaccgg cgccagggcc
     3061 ggaaaaacca cctgtcccaa ctgaccaggc cagacgactt gatgagccag gctggaccgt
     3121 caaagctggc agcttgatag cctctcaagg ccatcacctg actgatcaac ccggggtcat
     3181 tgaacatggc ccccagacgc ctgccgttca tgctgagctc ttgcacatgg agatgtcgca
     3241 ccaaccagcc caaaatgcgc caggacatgg caccacccaa cgcacctttc ggataaatgg
     3301 tgtcgagcag taccagacca cgaatcgcca cgccctgttg tctcagaagt cgggctgtct
     3361 ccagcgccgc taccccaccg actgaaaacc ctgcaataaa accgggcgca agccgttgat
     3421 tggcgatgct ggtcgcataa agcccggcca gttcggccac cgtcgcgacc gccttgtcgg
     3481 tgggtggttg cagcaactgc acgtcgcatg cgctgcctag cgcatgtgcc aggttctgaa
     3541 agcgcagcaa atcgccatgg ccggaggctg caaggtacag cggaacctgt ccactccgac
     3601 tgccgagttc aatcagaacg cttggtgcca tgacatcact tttcaatgcc agagccagtt
     3661 gctcaatggt cggatgctcg atcaataaat gcatgggcac tctgcgcccc agcagttttt
     3721 cgattcccgt caagatgctc accgcagcca gggagtcgcc gccgaggtca aagaaattgt
     3781 ccgcaagtcc gatcgtgggc gggttcagaa ccgcctgcca cagtgccatc aaatcgcgct
     3841 ccagagcaga cgcaggcaaa cgaacaccac tcaccagagg cactcggctg atttcaggca
     3901 atgcagcgaa atctactttt ccggcgctgg tttgtggcag cttgtcaagc acgcggatgc
     3961 ccgccggtat catgtagtcg ggcaaacggc tgcgcaaagc ctgttgcagg ctgtgcgagt
     4021 tgaccccagg gttggtggcc acccacacat ggattgccgg cttgccattg tctgcaacca
     4081 gttttgccgc ggcttgtaaa acgcccggta gtgccaggca tgtggcctcg atttcgccga
     4141 gctcaatgcg atagccgcgc agcttgattt gccggtcaat gcgcccgcaa aaatgcaggt
     4201 ttccttccag acccagccag cccagatctc cggtgcggta aattcgcgcg cccggctgaa
     4261 aggggtcatt cagaaaaacc tgcgcatcca gctcggggcg gttcaggtaa ccacgcgcaa
     4321 gcgccgcacc gccgataaat acctcgccgg tgacgcccag tggcatcagt tgcaactggg
     4381 cgttcatgac ataaatgcgt gtgtcatcaa tgggccgtcc aaccggcagg gcgacatcga
     4441 ccggatgaag ttcgcattgc caggcgctgg caaaaatggc cgcctcggta ggcctgtaca
     4501 cgttgtaaag gcgcgcaccc gtctcggtga tgaaccggtt gcccagttca ggcggtaaca
     4561 cctcaccacc gcaacatgca acacgcagct tcagtccggt cttgccgacc gcaccatcca
     4621 gaaatcgact cagggtggac ggtacaaacg ccatgatggt gaccccgtgg ccaatagcaa
     4681 aatccgccaa ggactcaggc aacaaccgcc ccggtggcgg aatggcaacg ctgcctccat
     4741 gaatcaaagg caaaaacagc tcaatgagcg aggggtcaaa agtgagctgt gtcgcctggg
     4801 ccgagcggtc gtgccagtcc actgcgtagg tgcgcgacag ccaggccaag cggcgcgaca
     4861 aggtggcatg cgtgaccatc acacccttcg ggtgcccggt tgaaccagag gtaaacagca
     4921 catatgccaa atcgtcaggc tggggttcta tccatgccgg gagcgtgtcc agccacacct
     4981 cgggcgttgg cggcgagttg ctgtcaaata ccagggtgtt gccatgcaag ttggcaaagc
     5041 gggccgcatc tgctttctcg atcaacaatg cgctagccgc gctgtcttgc agtatctgcg
     5101 cgatgcgcgc caccggcgcc tccggatcaa gcggcaaaaa ggcagccccg gccttggcaa
     5161 tgcccagcag cgccaccatc atgttgggcg atctggcaat ggcaactgcc atgatggcac
     5221 ccgcagccgt gccactggcc ttcaagcgcc tggaaagttg ctcggccttt tggtctagcg
     5281 ttgcatagtc catattgccg ccattccaga ccactgccgt tgcctcgggc cgcaaggccg
     5341 cctggtgttc aaactgggaa atataggttt gggtggcttc gtggctggca acagccttgt
     5401 gcagtccttg aagcagtgcc cattcttcct caggcggcaa cagcgaaata gcctccaaag
     5461 gggtgctggg ttcggccatc agtgcctgcg ccaaatgcca aagccgccgc cccagcaaag
     5521 ctggctcgcc ggtcgcaaag cagtcgctgc tggcatcaag caccagctca atatcctggt
     5581 caggatgaaa ctcacacacg gtcacaccca ggggatagcg ggcagtgccg gaaaaaatct
     5641 gcctcgagtc caccagcctt gccgaaccaa acgcggcaga gtaatcttgt cgctcaaaag
     5701 acagcagcac atcaaacagg ccgtcgcgcc ccgtgcgaat cagttgcaag gctttgccca
     5761 gctcgctcaa ggggtaacgt ggatgccgca acgcaccgcg cagggcgctg ccggtggccg
     5821 ctagcaaatc cgccaccgtc aggctttcag tgacgaccac cttcagcgcc agcacaccca
     5881 caaacatgcc cggcgttgag cggaaacgcc tgccggatcg gttcaggctg ggaatgccaa
     5941 gtaccacctc ctgaaagccg ttgatgcgtg aaaagtacag cgccaaagca gccagaaaaa
     6001 agttgaaagc cgtcgtcccc tgggtgctgg cgtaggcctc aagatgcgcg taatcgctgc
     6061 gcgggatttt ctgttttgcc aggcgtgatc tgggcaagtg ctcgggtctc agtggcgcgt
     6121 agcgccgcac caccagcggc tcgggcaggg tcggcatctg cgaccgccaa taggcggcat
     6181 ctcgctcaaa ggcctgcgag tcacggtact cgttggattc gtcaataaac tgcaggtagt
     6241 cggggcagtt tgtggattcg ggctgaaccc cggcatccag agcgttgtaa atctggctcc
     6301 agcgctgcat cacctgcgcc gtaccccagc cgtccatcac caagtggtga aactgaatgg
     6361 tcaggccatg cagttcatcg cttgcatgga gcagtgcaaa acgccagggt ggctggccgt
     6421 tgagcacaaa ggcttgctga atccgcttgt gccaccagac acgcatagtg gcctcggggt
     6481 tcgctgcgcc gctcaggtca acccattcca gcgtgggctc aaactgcgcc agcagcgcct
     6541 gggttccgtc aagcctgggt gccagtcgta gagcctcgtt ttcggcgacc agacatgaca
     6601 gtgccgcctt gaatcgagga aggtcaaagc ggccgcgcag gaacgcaccg ccaccgatat
     6661 tcaggtgcgc actgtctggc caggcgcact gatccagcca gacctcgcgc tggctcagcg
     6721 acaggcttac ttgccgggct cccaacgagc tgactgttgt tgtttccatt gcgcaatttt
     6781 tccttgggca taacgatatc cgacttcggc aatggcgtgc gcatgccgat agcccatgag
     6841 cgaaaatcca gccaccggtg gctccagaaa gtgatcggcc tgcgccatag tgcgacctcg
     6901 ttgactaacc gagccgacgt gcccgacgct gtaaagaatg tcagcaatgt ttggggacgg
     6961 atgggtgcct gggaagagcg agcccttgat gacctgccag ggcgacaagc gagccagtgt
     7021 cggatcagca cccaaggcat cgggaacatc cacatcaacc gcgatgatgg tgccgttgcc
     7081 atgacgcttt tccagcgcag tgcccaagcg tacccgcatc gcgctcaccg gcacgttgtc
     7141 aagaatggca ccgtccacca agagctcgcc attgtcaagc acgggcggga acaaacccgc
     7201 tggcgagttg ctggcaagca ctgcgcgcca cagcgggccg gtctcttgca cacaggtctt
     7261 gcccctgctg aggttgcagg cggcagcaaa atacggccgc cataggcctt caatcatggt
     7321 gtcgccaaac atgcgtttca ggtcacgctc tacccggcgg cctgatacca gtgagaccaa
     7381 cggcagcgtt gggtgttcgc cgccgccagc aaagtgctgg gtttgactca agatggcatc
     7441 cagcgaaacg ccactgacat attgcgcacc aatcagtgcc cccatgctgt tgccgccaac
     7501 caggtcgatg ggaatgccag cctcttccat tgcacgcaac acccccacat gggcaaaccc
     7561 gcgtgcgccg ccaccaccca acaccacgcc gacggcactg ccggtaagaa agcgcgccag
     7621 ccgggaaaaa tcgctttctt gctgggtgcg cagcgggtaa atacgctcat agcgcgcagt
     7681 gtcgcgccaa cgggcgacct cggtgggctc cagcgcagtg gcagggtgta gaagcaccag
     7741 atgcttgcgc ttcattggaa agcctagttc ctgcgtcaga ccctgctcaa tcacacccag
     7801 cgcctgcgag gcaccgaccg atgccacaaa caccacctga tcggcctggc gaaacgccct
     7861 ttgcgtccag accgacaaac ccggctcggc ttcgtagacc aaaaaatcat actcctcatc
     7921 caactggttg tcccattcgg cattcatcga agttggttcg gaactgcctc cttggggtgg
     7981 cttgaagtga tgtactcgtc cacgctgcgc aaacgccttt ttcaggtcag cggccacggt
     8041 accgacatct gcgccctcat gcaggccaat caccacatag gtttgggcat agcgctgttc
     8101 gaccacgggg cgactgtcac gggcaaaagg taagttcgat tgaacaaaca cacggctcaa
     8161 tgccagtgga tagcgaatca aaagggcctc aaaattggcc cggctcagca ccgccaggat
     8221 gctgtcgcgc atcgcagtta cgtcggcggt gcgcggctgc tgaagaatca ggcccgcctc
     8281 gcccacgctc tggccgggtc ggatctcgtt gtacaaattg acttggcctg cgaccgtgcg
     8341 tcgggataca cgcagtcctc cgtgtagcac aaacagcata tcgatcgatg gctcaccctc
     8401 gtgctgcacc gtcgtgccac cgcgcacact ttgaaactcg agcagttccg ccagatcttg
     8461 caaaaccggt tcctcgagcg aaccaaaggc ccgaccagcg cgcaagacct tgtaggcacg
     8521 ttcacgcaaa ttcatggtat gttttcctca catgacaatc gcgtcgacag caaccctgtc
     8581 cggctaactc tgatcgcctg gctgatatag ccaggccgaa accttctagt ccattcatac
     8641 tgcagatcac ctgccccaga cgctgatggg ctgggtatct tggtagtgcg ctttgcgcca
     8701 gattctgtca tcccatggta ttttcggggc gcggacaaag atcagcaggc actcgccacc
     8761 gacaccgcac tggcagcaat ggcagcaatg gcagtttgct tcagtaccgc cgcgagggtg
     8821 gctttgtccc tgtgctgcat tttgagttta agcaccaact gttgcatcga accccttgga
     8881 cattgtcgct caatgccgat tgaaaacgca actcggtgcg acccggaccc acgccgcaat
     8941 ttcggtcaat gggactgcct cgtttgactg cgcgctgcgc aattgcgcgc aacaccagtt
     9001 tcatgccagc ctcgcggcag gctcaatggt gcagccaatt ctcggagagt tttcgacaag
     9061 ctgcctacac agctcctgga tgaagtttcc ttcccaatca accgattttt ccccaaagtc
     9121 ccctgtaact tgatgtgttg tgggctgact acatgttgct ttgggtgatc agctcgaatg
     9181 cgcgtttttg attatggtta gggttggtgg tgatctcgaa aagcgggcac ctcagccgca
     9241 ccgccaggcg tgcggcgcgt tttgcgcaca attgcctgca gctcggccat caaggtctag
     9301 aaaccttgtg tgggcgtggc atctgcgatg cttatggcat cgcaccgtcg tcgtggccgc
     9361 atctgagcgc ttggccggtg ccaccgggtc tcgcgtgagc ctggcggtct gatccgcatc
     9421 ggtaacacca acatgcgcca gaccaaaagc aaaagacagc agggtatgcg gcagttgtaa
     9481 aaaggaggcc atgctttgcg cgggggtcgg gcccgcgcga ctggtcaagc aatgccagcg
     9541 gagtggcgat ggtctgcggg tagtcttgcg gcaaaaaaca tggcactgtt tatgggtggc
     9601 tacgctggcg caatcaccct ggcaatacca cacagctttc aggcccgcca atgtcggccg
     9661 ccctgcggcg atcagcatca cagcgcacaa tccccccatg aaagcccctc ccagactgca
     9721 acccctcatt gaagaaggcc tgatcgacag cgtggttcgc caactgatga gcggcaaaga
     9781 ggccaccgtg tttgtggtgc gctgcggtga tgagacccgc tgcgccaaga tttacaaaga
     9841 ggccaccaac cgcagctttc gccaggcggt ggactacacc gagaaccgca aggtcaaaaa
     9901 tacgcggcag gcccgtgcca tggccaaggg cagccggttt ggtcgccagg cccaagaggc
     9961 cgcctggcaa agcgccgaag tcgatgcact ttaccgcctg gccgccgccg gcgtgcgggt
    10021 gccgcagccg tacaactttc acgagggcgt gttgctgatg gaactcgtca ccgatgccca
    10081 gggcgatgcg gcaccgcgca tgaacgatgt ggctttcacg ccagtgcagg cgctggcaca
    10141 ccacggcacc ttgatggccg aggtggtacg catgttgtgc gctggggtgg tgcatgggga
    10201 tttgtccgag ttcaacattt tgctggcgta tacccccggc gacggcgatg acgcgggcac
    10261 cgacatcccg gtgatcattg acttgcccca ggcggtggac gctgccggca acaaccacgc
    10321 ccagcgcatg ctgctgcgcg acgtggccaa tctgcgtgat ttctttggcc agtttgcgcc
    10381 cgaattgctc gccacccatt acggcccgga gatctggcat ctgtaccagg ccggtctgtt
    10441 aacccaggac agcgttttga ctggtcactt tgagcaggcg caaggcaatg tcgatgtcgg
    10501 cggggtgatg cgcgagattg acgatgcgag ggccgaaaac gcggcgcgca aactgcgcat
    10561 gcagaccgcg accgcacctt gaggtctctg ggtcgggccc atcgcaagag tcgtcccggc
    10621 aatttgggcg atgtaaactt gaccggttga ccggttgacc ggttgaccgg ttgaccggtt
    10681 gttggcgtgg taattccatc tctaaatcat tcgtgcctat gcaccttggc gcagacattg
    10741 caaacgcaag gcaaggcgag acaacaacga cattgatggt atagatatgc aataaggcgg
    10801 cctttgaacc aaatcaactc atcagccatc ggttgagcac ccggtccttg cgttgaacgc
    10861 gtgacgatga tggttgcatg gcaggcttcg cgccatgcca gctcactcga ctcctaaccg
    10921 accttttgat actatgaatt taatagctac tcccgcacgt ctatcaacga ctggcagcac
    10981 atttgatgct gatttcaagg cgcgattgca ttggtcggcc caaaccgatg cggcaatcga
    11041 gcagcgcgtc gccgacattt tggtcgacgt gcagcagcgt ggcgatgcgg ctgtgctgga
    11101 ctacaccgcg cgttttgatg ggctgcaagc caccagcatg gcagagctgg agctgacgca
    11161 ggctgaactt caactcgcct ttgatggctt gccagaactg cagcggctgg cgctcgtggc
    11221 tgccgccgac cgggtgcgca gtttccacca ggcgcaacgc aaggccagtg gcgagagctg
    11281 gagttaccgt gatgcggacg gcaccctgct gggccagaaa gtcaccccgc tggaccgcgt
    11341 cggcatctat gtgccgggtg gcaaagcggc ttacccgagc agcgtgctga tgaacgccat
    11401 tccggctcac gtggcgggtg tcgcagagat catcatggtg gtgccgactc cggtgcgcgg
    11461 cagtgttgcc agcggcgggc aaggtggggc gctggatgcc aaacccgagc tcaaccggct
    11521 ggtgctggca gccgcctttg tggcaggcgt gacacgtgcc tttaccgttg gcggtgcaca
    11581 agcagtcgca gcgctggcct acggcacggc caccattcct aaagtggaca agatcaccgg
    11641 tcccggcaat gcctatgtgg cgagcgccaa aaagcgcgtg tatggcacag tgggcatcga
    11701 catgatcgcc ggccccagcg agattctggt gctggccgat ggcagtacgc cgccggactg
    11761 ggtggcgatg gatttgttca gccaggctga gcacgacgag ctggcccaaa gcattttgtt
    11821 atgccccgat gcggcctata tcgacctggt gcaagcgtcc atcaaccgct tgctgcccga
    11881 gatgccccgg cgcgacatca ttgccaaatc gctcaatggc cgtggtgccc tgatccacac
    11941 ccgcagcatg gaagaggcct gcgacatcag caaccgcatt gcgccagaac atctggaagt
    12001 cagcagccgc gacccgcaac gctgggagcc actgctaaag cacgctggtg cgatatttct
    12061 aggtgcctac accagcgaaa gcctgggcga ctactgcgcc ggcccaaacc atgtgctgcc
    12121 gaccagcggc accgcccggt tcagttcgcc gctgggcgtt tacgactttc aaaagcgcag
    12181 cagcctgatc gaagtcagtg aagctggcgc gcagcgcctg ggccagattg cgtcggtgct
    12241 ggcgcatggc gaaggtcttc aggcgcatgc gcgggccgcc gagatgcgac tgtttgcccg
    12301 aaatcgcggc tagcctggca gttttgcaga tgcggctggc acctgctgca gaccaacgcc
    12361 gcgtgcgctg ccattgctgt cagggaatcg agaggcgttc gccctctggg accacccggc
    12421 tgcgttcgcg accgttgacg ctgtaggtga tccacagttg ctttggccgc ttctcggccg
    12481 gatcaccgcc tgccatgttg ttatcgacct tgatattgag ccgcccgttg tacacgcgct
    12541 gctgcagccg ctcggcgatg tccaatcggt cgcggccggc gccatactcg gcacgcagaa
    12601 tgcgcaaacc ctggtcatag ttgtcgtagt tgccgccgcc gacagcaggg cggtcgccgc
    12661 cccaattgcc atcgccgcct tggccccaat tgccgctgcc ccagccactg aagtgtgagc
    12721 catcaaccca actgctctcg gtgtattcga aagtccgcat ctgcccgttt gagccgcgcg
    12781 cgtgaatgcg cagtgtcttg gtgcgaccgt gatccggatc gacaccgaag gtttcgttgc
    12841 tcagcagaaa gcgctcgttg cgccgcgcca gctcgcgcag gcgctgggtg acatcgacgt
    12901 ggcgatcggc ggtgccgtag cgtgcctgca gtattttgta gcccccattg ccaccgtcgt
    12961 gataacccgg ccggttcccc cagccaccct gaccctgctg accattgcct tgttcccagt
    13021 tgccaccgct ccagccgctg aattgcgcgc catcaaccca actgctttcg gtgtactcaa
    13081 aggtgcgggg tctgccgctg gggtcggtgg cgatgatgcg cagggtcttg gtgcgacctt
    13141 tgtccggatc gacgccaaag gtttcgttgc tcaagatgaa acgctcgttg ttggcggcga
    13201 gttcacgcag acgctgggtc acatccacgt tgcgttcggg ggtgccatag agggcacgct
    13261 gaatcgtgta ttcaggctgg ttccaggtgg cggccatagc cggtgccatc gggaccaccg
    13321 ccaaagcggc caaaatcgtc cctaaaccac tcacttcacg tcttctcatt ggagttgctc
    13381 ctattgcatt gttgaatccg ccgccaaact ttttccatcg acacccacgg cgctcaatga
    13441 tgatagcggc aaacgctgcc gaccaaacac cagcgattga gcgttagcgg ttgtcgcatt
    13501 gcctaaaata ctcaaaggaa acgtgcaaag cgcgcattgg ttgaaagacg caagttgtcc
    13561 gcaagggtgg tggcaaactt gaatgcgccg gacctcatgc aacaacacat caacaacaca
    13621 tcaacgacac actggcatga ctcttttgca atccattatt gactcccgca tccgttccga
    13681 cgtgcaagcc atgcacgcct atgttgtgca ggatgcaacc ggcatggtca agctcgatgc
    13741 catggaaaac ccgcatcgcc tgtcgccaca tctgcaagcc aagctgggcc agcgcctggg
    13801 tgcgctggcg ctgaaccgct acccggccga ccggctaaat gacctgcgcg ctgccctggc
    13861 ttgccatgcg cacatgcccg cggggttcga catcatgctg ggtaatggct cggatgaact
    13921 gatcagcctg ctggcgctgg cttgcgccgt gccaccgtcg acggccaatg gtttccagaa
    13981 agcctgtgtg ctggccccgc tgccaggctt tgtcatgtac gccatgagcg cgcagttgca
    14041 ggggctcgat tttgtcggtg tgccactgac ggcaagcttt gaattggacc tgccagccat
    14101 gctgaacgcc atcgcgcagc atcggccagc actcacttac ctggcttacc cgaacaaccc
    14161 gaccgccaat ttgtggcatg acgcagccat gcagtcggtc attgaagcag tggcagagca
    14221 gggcggtctg gtggtgcttg acgaggccta ccagccgttt gccagcgcga gctatatcga
    14281 ccgcttgtcc cggcacagcc atgtgctgct gctgcgcacc ctgagcaagt ttggtctggc
    14341 tggcattcgc cttggctatc tgatcggacc gcgtccgctg attgcccaga tcgacaaggt
    14401 gcgcccgccc tacaacgtca gtgtgctgaa ttgcgaggcg gccctgtttg cgctcgaaca
    14461 tcaagaggta tttcaggctc aagcccttga tgtgattgcg cagagagcta tgctttttga
    14521 ggcacttgga agattgcccg aagttcgggc gttccctagt caggccaaca tgatgctgct
    14581 gcgcgtaccc gatgccgaca tgacttttga gggcttgaag gcgcgtggtg tgctggtcaa
    14641 gaacgtttct aaaatgcacc cactgttgaa ccaatgcctg cgcctgacgg tcggcaccga
    14701 acctgaaaac cgccaattcc tggcagccct ggaagcctcc ctatgaccaa ctatccactc
    14761 accgaactcc ccgaagcttc gacctccagc gccccacgca aggcggagct gacccgcaac
    14821 accgccgaga cacaaatttc ggtgcgagtc aatctggacg gcaccggtgt ttcgcgcctg
    14881 cacaccggca ttggtttttt tgaccacatg ctggaccaga tcgcccgcca tggcctgatc
    14941 gaccttgaca ttgatgccga gggtgacctg cacattgatg cccaccacac ggtggaagat
    15001 gtcggcatta cgctgggaca agtggtcaat caggcggttg gtgacaaaaa aggccttcgc
    15061 cgttatggcc atgcctatgt gccgctggac gaggccttga gccgggttgt gattgatttt
    15121 tcagggcgac cgggcttgct gatgaatgtg ccgttcacca gcggcagcat cggcactttt
    15181 gatacccagt tggtgcacga gtttttccaa ggctttgtca atcacgcgtt tgtcacactg
    15241 cacatagaca acctcaaggg cgacaacgca caccatcagg tcgagagcgt gttcaaagcc
    15301 tttgcccgcg ccctgcgcag cgcagttgaa cttgacccgc gtgcgctggg gcaaatcccc
    15361 tcaaccaagg gaagtctgta ggtttaaatg aacaattggc ctgtagcccc cgtcctgatt
    15421 gcgtaagtag ctatgatttc aatagtaagt agcagcgcta aaaaaaccgt cgccgtggtc
    15481 gattacggca tgggcaacct gcgctcggtc accaacgcgg tcatggctgc tgcccgtggc
    15541 ataccggtcg aggtggtctg gacccagcgc cccgaagagg tcatggctgc tgatcgggtg
    15601 gtgctgcctg gccagggcgc aattcgcgac tgcatgcgcg agctgcgtga ctccggcatg
    15661 tacccggcgg tcatgcacgc gctgatcaac aagccggtga tgggggtctg tgtcggtatg
    15721 caaatgctgc tggaccacag ccacgaaggc tacgccggtg atgcgggcca gggcacgccg
    15781 tgcctgggtc agatccacgg cgaggtgatc aagttcgagt tggccgggca gttgcagccc
    15841 gacggcagcc gctacaaggt tccgcaaatg ggctggaatc aggtttatca aaacagccac
    15901 ggggcaacgc cacacccgat ctgggacggg gtgcctgacg gttgctattt ctactttgtg
    15961 cacagctttt acgccaaacc gtcagatgcg cgccacagcg tgggcgagac ggactatggt
    16021 caacgcttca gttcggccat tgcgcgtgat aatatttttg caacacaatt tcaccccgaa
    16081 aaaagcgctg aacacggctt gtccctgtac cgcaactttt tgcactggaa cccctgagca
    16141 caatttttgt cttccatgaa cacgaagcga caccccacac gatgaaccat cgttgtcatt
    16201 cccgcacagg tgccacgcca tgtccccaga gtcttctgcc caacgcccat gacggtcatc
    16261 attgacctgt cacttcatcc cgttttcttc ttccagccta aagcatcatg ctgttaattc
    16321 ccgctatcga tctcaaagac ggtcactgtg ttcgcctgca gcagggcgac atgaaacttg
    16381 cgactgtctt cagcgaagaa cccgtcgtga tggcgcgcag ttgggtggac aagggtgcgc
    16441 ggcgcctgca tttggtcgat ttgaatggtg cgtttgccgg gcatccgaaa aacgaggcag
    16501 cgattcgcaa gattctccag gacgtgggca gcgaggtcga tgtgcaattg ggcggtggta
    16561 tccgggacct cgacaccatc gagcgttatc tggatgccgg cctgcgctac gtcatcatcg
    16621 gcacggccgc ggtgcgcaac cccggcttct tgcaggat
//
LOCUS       DAOSLL010000018        16031 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_3813_length_16031_cov_4.585566, whole genome shotgun sequence.
ACCESSION   DAOSLL010000018 DAOSLL010000000
VERSION     DAOSLL010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 16031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16031
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3813_length_16031_cov_4.585566"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="PLB25_01440"
     CDS             <1..281
                     /locus_tag="PLB25_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOE40289.1"
                     /translation="GAMVGVIGTLAGLLLGLGVAFNIDVIVPALEQLLGASFLPRDIY
                     LISRMPSDPQQADILPITLISLILAFIATLYPSWRASRVNPAEALRYE"
     gene            274..993
                     /gene="lolD"
                     /locus_tag="PLB25_01445"
     CDS             274..993
                     /gene="lolD"
                     /locus_tag="PLB25_01445"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HOE40290.1"
                     /translation="MNEATMPVSSGTVLSARGLSRRFTEGRLDVTVLQGVDLDVRAGE
                     TLAVVGASGSGKSTLLHLLGGLDAPTSGSVSLLGQDLARQSATQQGRLRNQHLGFVYQ
                     FHHLLPEFSALENVALPLWIRRVAHIDAALEATKILASVGLKERLQHRPSELSGGERQ
                     RVAIARALVTRPACVLADEPTGNLDRSTAHGVFDLMLELARNQGTAFVLVTHDTALAA
                     RCSRQLTLVQGCLQESLAKPA"
     gene            complement(980..3013)
                     /locus_tag="PLB25_01450"
     CDS             complement(980..3013)
                     /locus_tag="PLB25_01450"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE40291.1"
                     /translation="MLNNQPVTQKEYILRDNQSPISRTDAKGRITYANADFCEAAGFT
                     EDELIGKAHNVVRHPDMPEEVFADMWRDLPKGLTWTGMAKNRRKNGDHYWVVANVSPI
                     WENGQITGYASVRMKPDRELIAPTEAIYQRFRQGQAKGLRIEHGKVVRTGLPGLLDLW
                     RQPNIQRRLTLLLALAALLIVAVGWLGLYGMNATNQQVQAVYREGARAIAHLDVIARL
                     QLRNQLALSSALANDEVDFKQADKAQTLATQIEADISKINSEWSSYLAIGHTAQERQV
                     QTEFEQIWAQYVNEALKPAVAALRKGDFALVARTYRYTAVRHFEALNTNLNAQLLRQD
                     ADAKQAVTDAQSRADWLLLVGAIGIVLGLLLLFGLGWRLQRSVLAPINRAVAISKQIA
                     AGFLGNRIDRQGNDEPGQLMSALHAMQVSLSSMAQTILISAESVSSEANAISQSNEAL
                     AGRTEEQSLSLKETSNNMETVSATVQRNTDNAQTANRLVQEAGAIVTDGGSAISKVVG
                     TMDSIARSSRRITDIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRLLA
                     GRSAAAAKEIKTLIEDSVQQVEGGMVQVSEARQTIDSSIDAVHRVAALMAEISTASHE
                     QGQAIDRVAQLVAQIDQATQQNVPMAENAASTARNLGEQGNELVRTAGVFRLA"
     gene            complement(3078..3236)
                     /locus_tag="PLB25_01455"
     CDS             complement(3078..3236)
                     /locus_tag="PLB25_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40292.1"
                     /translation="MSVAPCQPGVVLKAADAGARDRSLARGPGRPEYRFAAQFWLNIK
                     KAIKSAIR"
     gene            3267..4346
                     /locus_tag="PLB25_01460"
     CDS             3267..4346
                     /locus_tag="PLB25_01460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE40293.1"
                     /translation="MSLPEIKSRVLVVEDELVVARDIEQQLTDLGYLPVAIATRGEEA
                     IDLVASLHPNLVLMDIQLAGSMDGIAAAQIIRDKFALPVVFLTAFSADEVLARAKLAE
                     PFGYILKPFSERELRTVLAMALYKHQAEAKLQNSTRQLKALSRRVLEAQESERRRVAI
                     ELHDELGQSLTAIKINLQLNERFKDKSPVDFNQESIRIVEDALHQVRRLATALRPSML
                     DDLGLAPALKWITEQTAQRVGFAVSFHHERVQVRLAPEIETACFRIVQEALTNVARHA
                     QASQVDIRLEREGNDLLLTVQDDGQGFDMAAMRERAVSGGSLGVLGMQERATLLGGSL
                     DIVSCPGGGSTLQLRCPWRTQSEAA"
     gene            4343..4990
                     /locus_tag="PLB25_01465"
     CDS             4343..4990
                     /locus_tag="PLB25_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE40294.1"
                     /translation="MRTTRVLLADDHTLVRAGLRKLLESIPDIDVVGEAGDGLALLAL
                     AERLQPDLVLMDIAMPGLNGIEATARLHKTQPQVRVLMLSMHQNEEYVRQALRHGAAA
                     YLLKDSAPLELEMALAAVLRGETYLSPAVTKGVLQDYVHRLRGDEQPSEQLSPRQREV
                     LQLIAEGHSTKEIARRLDLSIKTVDTHRSQLMKQIDIHEVAGLVRYAMRVGLVSI"
     gene            5056..5205
                     /locus_tag="PLB25_01470"
     CDS             5056..5205
                     /locus_tag="PLB25_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40295.1"
                     /translation="MLDARLTTVGGFVFIACASIRLAQQSQQIKISVRYFSAQNQVFA
                     VCQFC"
     gene            5225..7366
                     /locus_tag="PLB25_01475"
     CDS             5225..7366
                     /locus_tag="PLB25_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE40296.1"
                     /translation="MLKKSDLSLPSPAGRATLSSSRILIVEDEVIVARDICQQIESMG
                     YQSVGMATSGEQAVLLAGQLRPDLVLMDIELGSGMDGIAAALAIRASQPLPVVFLTAY
                     SADDVLARAKLAEPYGYILKPFSEHELRSVLQMAIYKHQADARQRDLALHHQAILDNL
                     ASGVVSVNACGVIESFNKGACAMFGYTPEQVLGYNMSMLMPESAREMHADILRLGPTQ
                     GVDRVVCREREVMGLRRDGTLFPLSLTVTNISRAGQPVFLGILSDLSQQRLDEEKIHQ
                     LAFYDELTGLPNRRRLLDRLKLAIVASGRSGQHGALLFLDLDHFRQINETMGPGFGDD
                     LLIEVARRLLACVREDDTVAHLGGDEFMVMLEELGLGRDTAAVHAEMVASKVLTSLSA
                     AYSLRGRAHTTTASVGIVIFDKSSGDLDELLKMADAAMYQAKGAGRNALRFFDPAIQA
                     QALARNKLEKDLQRGLQAQEFVLHYQIQVDASGAPVGAEALVRWMHPQLGMVPPGQFI
                     ALAEETGLILQLGQWVLQSACRQLATWSQLPGLGHWVMAVNVSSLQFAQDDFVDSVAQ
                     AIEAAGADPLRLKLELTESMLVHDVADVIEKMRNIRALGVSFSLDDFGTGYSSLTYLK
                     RLPMNQLKIDQSFVRDVLIDNDDAVIARTIVALGHNLGMSVIAEGVETTGQRDFLASM
                     GCDAFQGYLFGRPVPHLELLAFAVTEGILGL"
     gene            7401..10094
                     /locus_tag="PLB25_01480"
     CDS             7401..10094
                     /locus_tag="PLB25_01480"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE40297.1"
                     /translation="MSKLACLLSLLLWAMAGAAGALAPAALTPPASITVVLDDNYPPY
                     VFRDSNGRVQGVLVDTWALWQARTGIKVNLLAMDWLAAQQTMQTGQAQVIDTMFKTPE
                     REKIYDFSAPYADLEVPLFFHKSIGGIVDARSVRGFTVGVKDGDACIEVLQSHGADSF
                     RLYASYSSVVEAAATGELRVFCMDQPPATYLLNQRGIADQFRHSPAISVGKFHRAVRK
                     GDAALLATLEAGFAQITEAERLQINDKWHGAALQVLGGFPVSTVLQYALAVAALLTVL
                     LLLWNLSLQRRVQQKTRGLSQSLAELSLAKQVSEQSLSQLKTMLDNDMVGIVRVINRV
                     IWWSNPAFQNMFGYQADELIGKSTQMFYATVEDCMRLADAAYPLMSVGQIYRGQTEFV
                     HRSGQRLWVELSGQELTRSTGESLWIFLDITERKRAEALREETASRLEKLANSVPGLV
                     YQYLLRPDGSVALPYASNGLWDVFQLQSQMVTQDASAIFAKVHPDDIQAITDSIQESA
                     RNLTPWQHEFRVRHDDRTVRWLYGNSLPERLEDGSVLWHGFVTDITERKATEDRLRQL
                     SRSVEQAPVTIVITDLKGSILYANPSFTRSTGYSLAEAYGKNPRILKSGLTPPEVYRV
                     LWETLIAGGVWQGELHNRKKNGDLYIENAIIAPVQDANGMPSHYVAIKEDITQRKQAE
                     QAFQATLQEKVALLHEVHHRVKNNLQVISSLLRLETGRSIEPETRAVLKEMQGRILAM
                     ALLHETLYRVGSFASVELGDYLKQLASQAFRAQSSQGMLVRLVLDLAAVQVVMDQATP
                     CGLLVNELISNSLKHAFPAGRSGQVSIGLQAVPAPDGNPDLLRLWVSDTGVGLPPDFE
                     ARCRQSLGMQLVTDLARQLGGALQIGPGPGSEFAVTFVRQS"
     gene            complement(10135..13644)
                     /locus_tag="PLB25_01485"
     CDS             complement(10135..13644)
                     /locus_tag="PLB25_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE40298.1"
                     /translation="MKIYWFCWRSLKTRVTMMTLGIVLLGMVSLAWYVSSMLREDLES
                     LQGNQQFSMVTLVAADMDREIARHLAALQFAAATLTPALIANPVALGQHLTQRQDLVS
                     RFNNGIAVYQPDGSAITAIPSASGSPNHSDVVREDLIDVFKGAKAVVGQPHLSWPNRF
                     AAFVMSVPVLDPAGQVIGALSGATDLSGVNFLDKYVGNSYGASGSFVLIAPRSRLVVT
                     ATDKRRVLEQLPMPGINPEMDRLLLGFEGFSVAVGANGQQVLAAAKGVPTAGWQLAVL
                     LPTAEAFAPFVRLQTRTLLATLLFTLVAGTLIWWLLKRELSPMVSAVQTLANLADSDQ
                     APQPLPIARQDEIGKLIGSFNRLLATLKHRELFLQQILDTSSVAIYLIDAEGRINHVN
                     RRMSDMFGCSAEALNGRDYVSLLHPSLQDAGYKNMQALLANTINSSEVDRIYCRADQS
                     EFWGHLTCRSFDDASGKGRFIVCVINDITEGKKVHTALKESENRYSTLIEWSPDAALV
                     HRDGVLVYANGAAIALFGANAMSDLVGRNLIDLVAPEAQAFVRQRMQRQNEQGGSLPR
                     AETRFVKLDGSIIDVEVQATPIIYDGQRTNYSIIRDITAIKRTQAELSIAATAFESQQ
                     GITITNAQGTILRTNQAFTRITGYSAEEALGQNPRLLASGRHDAVFYQMMWKSLTIKG
                     NWQGEIWNRRKDGEIYPEWLSISAVKDSAGQVGHYVATFFDISARKSAEEQINNLAFY
                     DPLTQLPNRRLLLDRLAQALITCSRHRSCGALLFVDLDNFKTLNETLGHFQGDMLLES
                     VARRLSACVREGDTVARLGGDEFVVMIEHLSDNAVDAATQSEAVAEKIRLSLNRSYTI
                     GSYEHHSTPSIGITLFGEAPHETIDEPLKRAELAMYQAKAAGRNALRFFDAQMQVVVT
                     QRAALESDLSEALLQDQLLLHYQAQVVGDGRLTGAEVLVRWRHPERGLVSPAEFIPLA
                     EETGLILPLGQWVLETACRQLVTWSARPDLAHLTLAVNVSAKQFHQPDFVARVQSVLV
                     RTGVNPKRLKLELTESLMINDVEGIITKMTSLKASGVGFSLDDFGTGYSSLSYLKRLP
                     LDQLKIDQGFVRNILTDPNDAAIAKMVVALAESMGLSVIAEGVEIEAQKDFLAHLGCH
                     AYQGYLYSRPLALAEFERFTQQA"
     gene            complement(13893..15644)
                     /locus_tag="PLB25_01490"
     CDS             complement(13893..15644)
                     /locus_tag="PLB25_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE40299.1"
                     /translation="MFNLSSMPVARRLGLLTLSAVLGIVILASIFLVSERRLVLEERQ
                     NGVKQLVEIGYDMLKHSQKMVADGKVSEADAKQRAMSVIGALRYGNGEYFWINDMQPK
                     MVMHPIKPELDGKDLTDNKDPDGKYLFRAFVETVKTSSSGSGYVPYMWPKPGSPNPVP
                     KISYVKGFAPWGWIIGSGVYIDTVDAVMVQRAIGFAVGTMILVLVMLGLGVLISRSVL
                     RQLGGEPAYAAEITRRIADGDLAVEIAVPNPEIASLLQALAVMRDKFARIVGQVRQGS
                     EGVATASTEIAQGNNDLSSRTESQASALQQTAASMEQLSTTVKQNADHAVQANQLALS
                     ASTVAVQGGAVVAQVVDTMKGINASSRKIADIIGVIDGIAFQTNILALNAAVEAARAG
                     EQGRGFAVVASEVRSLAGRSADAAKEIKALINDSVTRVEQGSALVDQAGSTMNEVVSS
                     IRRVTDIMGEISAASGAQSAGVSQVGSAVTEMDKMTQQNAALVEQMAAAAGSLKAQAH
                     DLVQVVAVFKLDSHNTAQHLPSAMRASKRRSISPARPARPAVLRPSGVPAARALPPAK
                     AVKPASADGNDADWETF"
BASE COUNT         3042 a         4813 c         4841 g         3335 t
ORIGIN      
        1 aaggtgccat ggtgggtgtc attggcaccc tggccggcct gctgctgggc ctcggtgttg
       61 cgttcaatat tgatgtcatc gtgccggcgc tggagcagtt gctgggggcc agcttcctgc
      121 cgcgcgacat ttacctgatc agccgcatgc cgagcgaccc gcagcaggcg gacattctgc
      181 cgatcacgct gatctcgctg attctggctt ttatcgccac cttgtacccg agctggcggg
      241 ccagtcgcgt caacccggcc gaggcactgc gctatgaatg aagccaccat gccggtcagc
      301 agcggcacgg tcctgagtgc gcgtggcctg agccgacgct tcaccgaggg gcggctggac
      361 gtgaccgtgc tgcaaggtgt ggacctggat gttcgcgccg gtgaaacgct ggccgtggtc
      421 ggggcatctg gctctggcaa aagcactttg ctgcacctgc tgggcgggct tgatgccccc
      481 accagcggat cggtcagcct gctcgggcaa gacctggcgc gccagagtgc cacacagcag
      541 ggtcgattgc gcaatcagca tctgggcttt gtttaccagt ttcaccactt gttgccggaa
      601 ttcagcgccc tggaaaatgt tgccttgcca ctatggattc ggcgtgtagc ccatatcgat
      661 gctgccctgg aagctacaaa aatactagca agtgtgggct tgaaggagcg cctgcagcac
      721 cggcccagcg agctttcggg tggtgagcgc cagcgggtgg cgatagcccg tgcgctggtg
      781 acccggccgg cctgtgtgct ggctgacgag ccaaccggca acctcgaccg cagcaccgcc
      841 cacggggtgt ttgatttgat gctggaactg gcgcgcaacc agggcaccgc ctttgtgctg
      901 gtgacccacg acactgccct ggcggcgcgt tgttcacgcc agctcacgct ggtgcaggga
      961 tgtctgcagg agtcattggc taagccagcc tgaaaactcc tgccgtgcgc accagttcgt
     1021 tgccctgttc acccaggttg cgtgcggtgc tggcggcatt ttcggccatc ggcacgtttt
     1081 gctgggttgc ctggtcaatc tgcgccacca gttgcgcgac ccggtcaatg gcctgtccct
     1141 gttcatgcga tgcggtcgaa atttcagcca tcaacgcggc aacccggtgc accgcgtcaa
     1201 tcgacgaatc aatcgtctgg cgcgcctcac tgacctgcac catgccacct tcgacctgct
     1261 gcaccgagtc ttcaatcagc gtcttgatct ctttggcagc ggcggcgctg cgcccggcca
     1321 gcaagcgcac ctcactggcc accacggcaa agccgcggcc ctgctcgccg gcgcgggccg
     1381 cttcaacggc agcgttgagg gccaggatat tggtctgaaa ggcaatgccg tcaatcacgc
     1441 cgatgatgtc ggtgatgcgg cgcgagctgc gcgcgatgct gtccatggtg ccaaccacct
     1501 tgctgatggc gctgccgccg tcggtgacga tggcaccggc ctcttgcacc aggcggttgg
     1561 cggtctgggc gttgtcggta ttgcgctgca ccgtggcaga cacggtctcc atgttgttac
     1621 tggtttcctt gagcgacagc gactgctcct cggtgcgccc ggccagcgct tcgttgctct
     1681 gactgatcgc gttggcctcg ctgctgaccg attcggcgct gatcaaaatg gtttgcgcca
     1741 tcgatgacag gctgacttgc atggcatgca gggcgctcat caactggccg ggttcatcgt
     1801 tgccctggcg gtcaatgcgg ttacccagaa aaccggcggc aatctgcttt gaaatggcca
     1861 cggcgcggtt gatcggtgcc agcaccgacc gctgcagccg ccagcccagg ccaaacaaca
     1921 gcagcaaccc cagcacaatg ccgatggcac cgaccagcag cagccagtca gcccggcttt
     1981 gcgcgtcggt aacggcctgc ttggcatcag cgtcctggcg caacagttgc gcattcagat
     2041 tggtgttgag cgcctcgaaa tgccgcacgg cggtgtagcg gtaggtgcgc gccaccagcg
     2101 cgaaatcgcc cttgcgcaag gctgccacgg caggcttgag cgcctcattc acatactgcg
     2161 cccagatctg ctcgaactcg gtctggacct ggcgttcctg cgctgtgtgg ccgatggcca
     2221 ggtagctgct ccactcgctg ttgatcttgc tgatgtcggc ttcaatctga gtcgccaggg
     2281 tctgcgcctt gtcggcctgc ttgaagtcca cttcgtcgtt ggccagcgcg ctcgacagtg
     2341 ccaactggtt gcgcagttgc aagcgggcga tgacatccag gtgggcaatc gcgcgggcgc
     2401 cctcccggta caccgcctgc acctgctggt tggttgcatt catgccatac agtcccagcc
     2461 agccgaccgc cacgatcagc agtgcggcca gcgccagcag cagggtcagg cggcgctgaa
     2521 tgttcggctg tcgccacagg tcaagcaggc cgggcagacc agtgcgcacc actttgccgt
     2581 gttcaatgcg cagccccttg gcctggcctt gccgaaagcg ctggtaaatg gcctcggttg
     2641 gtgcaatcaa ctcgcgatca ggcttcatcc gcaccgacgc gtaaccggtg atctggccgt
     2701 tttcccaaat cggcgacaca ttggccacca cccagtaatg gtcgccattt ttgcgccggt
     2761 ttttggccat gccagtccag gtcagccctt tgggcaaatc gcgccacatg tcggcaaaga
     2821 cctcctcggg catgtccggg tggcgcacta cgttatgcgc cttgccgatc agttcgtctt
     2881 cggtaaaccc ggccgcctcg caaaaatcgg cgttggcgta ggtgatgcgg ccttttgcat
     2941 cggttcggga aatgggcgac tggttgtcac gaaggatgta ctctttttgc gttaccggct
     3001 gattgttgag catcttgttt tccttggcaa aggttgaatc ctgtgtgaat gggcacctgc
     3061 cgttgcaggt tgctgtctca tcgaatggcc gactttatag cctttttgat gttcagccaa
     3121 aactgcgccg caaaccggta ttcgggtcgg ccaggccccc tggccagcga tctatcgcgg
     3181 gcacctgcat cggcggcttt gagcacgaca ccgggttggc agggcgctac actcacaaat
     3241 cagatatatc ctaaccggag tgttcaatgt ctttgccgga aatcaagtcc agagtccttg
     3301 tcgttgaaga cgagctggtg gttgcgcgcg acattgagca gcaactcacc gatctgggct
     3361 acttgccggt cgctatcgcc acccgtggcg aagaggccat tgatctggtt gccagcctgc
     3421 acccgaatct ggtgctgatg gacattcagc tcgccggtag catggacggc attgcggcag
     3481 cccagatcat ccgcgacaaa tttgccttgc cagtggtgtt tttgaccgct tttagcgccg
     3541 atgaagtgtt ggccagggcc aagctggctg agccttttgg ctatatcttg aagccttttt
     3601 ccgagcgcga actgcgcacc gtgctggcca tggcgcttta caagcatcag gccgaagcaa
     3661 agctgcaaaa cagcacgcgc cagctcaagg ccttgtcgcg gcgggtgctc gaagcgcaag
     3721 agtcggagcg ccgccgggtt gccattgagc tgcacgatga gctgggccag tcgctgaccg
     3781 ccatcaagat caatctgcag ctcaacgaac gtttcaagga caagtcgccg gttgacttca
     3841 accaggagag cattcgcatt gttgaagatg cgctgcatca ggtgcgccga ctcgccactg
     3901 cactgcgccc gtcgatgctc gacgatttgg gtctggcccc ggcgttgaaa tggatcactg
     3961 aacaaaccgc ccaacgcgtc ggttttgcgg tgagttttca ccacgaacgg gtgcaggtgc
     4021 ggctggcccc cgagattgaa acagcctgtt ttcgcattgt tcaagaggcg ctcaccaatg
     4081 tcgcccgcca tgcgcaggcc agtcaggtcg atatccgtct ggagcgcgag ggcaatgact
     4141 tgctgctgac ggtgcaagac gatggccagg gctttgacat ggcggccatg cgtgagcgcg
     4201 ccgtttccgg tggtagcctg ggcgtgctgg gcatgcagga gcgcgccacg ctgctgggtg
     4261 gttcgctgga catcgtgtct tgccccggcg gcggcagcac attgcagctc agatgcccat
     4321 ggcgcaccca gtcggaggca gcatgaggac aacgcgggtg ctgctggccg atgaccacac
     4381 cctggtgcgg gccgggttgc gcaagctgct cgaatcgatt ccagacatcg atgtcgtggg
     4441 cgaggccggt gacggcctgg ccctgctggc tttggccgag cgtctgcaac ccgatttggt
     4501 gctgatggac attgccatgc ctgggctcaa tggcattgag gccaccgccc ggctgcacaa
     4561 gacgcaaccg caggtgcggg tgctgatgct gtcgatgcac cagaatgaag agtatgtgcg
     4621 ccaggcgctg cgccacggtg cggccgccta tttgctcaag gattcagcac cgctggaact
     4681 cgaaatggcc ctggcagcgg tgttgcgcgg ggaaacctat ctgagcccgg cggtcaccaa
     4741 aggtgtgctg caagactatg tgcatcgtct gcgcggtgac gaacagccca gcgaacagtt
     4801 gtcgccgcgc cagcgcgaag tgctgcaact cattgccgag ggccacagca ccaaggaaat
     4861 cgcccgccgt cttgatctgt caatcaagac cgtggacacg caccgcagtc agttgatgaa
     4921 gcagatcgac atccatgagg tcgccgggtt ggtgcgttat gccatgcggg ttggactggt
     4981 ttctatatag aaaattggcc tctagcgctt ttgcagcatg cgctggtagc tatatatttt
     5041 gaagcaaact gaggcatgct cgacgcgcgc ctcacgaccg tcggtggatt cgtcttcatc
     5101 gcctgtgcgt caatccggct tgcgcagcaa tcacagcaga tcaaaatttc ggtcaggtat
     5161 ttttctgcgc agaatcaggt gtttgccgta tgtcagtttt gctgacaatg acttatcgtg
     5221 catcatgctc aaaaagtcag acttgtcctt gcccagccct gctggtcggg ccaccttgag
     5281 ttcaagccga atcctgattg tggaagacga ggtcatcgtc gcacgcgaca tctgccagca
     5341 aattgagtcg atgggttacc agtcggtggg catggccacc agtggcgaac aggcggtgct
     5401 gctggcaggc cagttgcggc ccgatctggt gctgatggac attgagcttg gcagcggcat
     5461 ggacggcatc gctgccgcat tggcgattcg tgccagccaa ccattgccgg tggtgtttct
     5521 gacggcttat tcagcagacg atgtgctggc gcgggccaag ttggccgagc cctacggcta
     5581 catcctcaaa ccgttttccg agcacgaact gcgcagcgtg ctgcagatgg cgatttacaa
     5641 acaccaggcc gatgcccgcc agcgcgatct ggcgctgcat caccaggcga ttttggacaa
     5701 tttggccagc ggtgtggtta gcgtcaatgc ctgtggtgtg attgaatcgt tcaacaaggg
     5761 tgcctgtgcc atgtttggct atacacctga gcaggtgctg ggctacaaca tgtcgatgct
     5821 gatgccagag tcggcgcgcg agatgcacgc cgacatcttg cgcctgggtc cgacccaagg
     5881 cgttgaccgg gtcgtttgcc gtgagcgcga agtcatgggt ttgcgccgcg acggcacgct
     5941 gttcccgctg agcctgacgg tgaccaacat ctcgcgcgcg ggccagccgg tatttcttgg
     6001 cattctcagt gacctgagcc agcagcgcct tgacgaggaa aaaattcacc aactggcgtt
     6061 ttacgatgag ctgaccggcc tgcccaaccg ccgccgcctg cttgaccggc tcaaactggc
     6121 gattgttgca agtggccgct ccggccagca tggtgccctg ctttttctcg accttgacca
     6181 tttccgccag atcaacgaaa ccatgggccc cggatttggt gacgatttgc tgattgaagt
     6241 ggcccggcgc ctgctggctt gcgtgcgtga ggatgacacc gtggcacacc tcggcggcga
     6301 cgagttcatg gtgatgctcg aagagcttgg gctgggccgc gacactgcgg cggtgcatgc
     6361 agaaatggtt gccagcaagg tactgacctc actcagtgcc gcatacagtc tgcgcggacg
     6421 cgcccacacc acgacggcca gcgtcggtat tgtgatcttt gacaagagtt caggcgacct
     6481 cgacgaactg ctcaaaatgg ccgatgccgc gatgtaccag gccaagggtg ccggacgcaa
     6541 tgcattgcgg ttttttgacc ctgccatcca ggcgcaggcg ctggcccgca acaagcttga
     6601 aaaagacctg cagcgcggcc tgcaggcgca ggaatttgtg ctgcactatc aaatccaggt
     6661 cgatgccagc ggtgcccctg tgggggccga ggcgctggtg cgctggatgc acccccaatt
     6721 gggcatggtg ccgccgggcc agttcatcgc gctggccgaa gaaaccggcc tgatcctgca
     6781 gcttggccaa tgggtgctgc aaagcgcttg tcgccagctt gcgacgtgga gccaactgcc
     6841 cggcctggga cactgggtga tggcggtcaa cgtcagttcg ctgcagtttg cgcaagacga
     6901 ttttgttgac agcgtggcgc aagccattga agcggccggg gctgatccgc tcaggctcaa
     6961 gctggaactg actgaaagca tgctggtcca cgacgtggcc gatgtgatcg aaaaaatgcg
     7021 caacatcagg gccttggggg tgagcttttc gctcgatgac tttggcaccg gctactcgtc
     7081 gctgacctac ctcaagcgcc tgccgatgaa ccagctcaag attgaccagt cgtttgtgcg
     7141 cgatgtgctg atcgacaatg acgacgcggt gattgcccgc accattgttg cgctgggtca
     7201 taacctgggc atgtcggtga ttgccgaggg ggtggaaacc accggccagc gggactttct
     7261 ggccagcatg ggttgcgatg cctttcaggg ttacctgttt ggtcgcccgg tgccgcacct
     7321 ggaactgctc gcttttgccg tgactgaagg cattttaggc ctctagccct tatgctacct
     7381 gcacaagcag ctatataaaa atgagcaaac tggcttgcct gctgagcctt ttgctgtggg
     7441 ccatggcagg ggcagcaggc gcgctggcac ctgctgccct gacaccgcct gcgtccatca
     7501 ccgtggtgct ggacgacaac tacccgccct acgtgtttcg cgacagcaac ggccgggtac
     7561 aaggcgttct ggttgacacc tgggccttgt ggcaggcccg caccggtatc aaggtgaacc
     7621 tgctggcaat ggactggctg gcggcccagc agaccatgca gacgggccag gcgcaggtga
     7681 ttgacacgat gttcaagacc cccgagcgcg aaaaaattta cgacttcagc gcaccctacg
     7741 ccgacttgga ggtgccgctg ttttttcaca aaagcattgg cggcattgtg gatgccaggt
     7801 cagtgcgtgg ttttaccgtc ggggtgaaag acggtgatgc ctgcattgaa gtgctgcagt
     7861 cgcacggggc cgacagcttc cggctctatg ccagttattc cagcgtggtc gaagcggcag
     7921 ccacaggcga actgcgggtg ttttgcatgg accagccacc tgccacctat ttgctcaacc
     7981 agcgcggcat tgccgaccaa ttccggcact cgccggcgat ctcggtcggc aagtttcacc
     8041 gtgccgtgcg caagggcgat gcagccctgc tggcaaccct ggaggcaggg tttgcccaga
     8101 tcaccgaggc cgagcgccta cagatcaacg acaagtggca tggtgcggcg ctgcaggtac
     8161 tgggcgggtt cccggtcagc acagtgctgc aatacgcgct agcagtggcg gcgctgctga
     8221 ccgtgttgct gctgctgtgg aacctctcgt tgcagcggcg cgtgcagcag aaaacccggg
     8281 gtttgtcaca gtcgctggcc gagttgagtc tggccaagca ggtgtcggag cagtctctgt
     8341 cccagctcaa gaccatgctt gacaacgaca tggtcggcat cgtgcgcgtc atcaatcggg
     8401 tgatctggtg gagcaatccg gcctttcaga acatgtttgg ctaccaggcc gacgagttga
     8461 ttggcaagtc cacgcagatg ttctacgcca ctgtagagga ctgtatgagg ctggccgatg
     8521 ccgcctaccc gctgatgtcg gtgggccaga tttaccgcgg ccagaccgaa tttgtgcacc
     8581 gcagcggtca gcgcctgtgg gttgaattga gcggccagga gctgacccgg tcaaccggtg
     8641 aatcgctgtg gatttttttg gacatcaccg aacgcaaacg cgccgaagca ctgcgcgaag
     8701 agaccgccag ccgcctggaa aaactggcca attcggtgcc tggcttggtt taccagtatc
     8761 tgctgcgccc ggacggcagt gtcgccctac cttatgccag caacgggcta tgggatgttt
     8821 tccagcttca atcgcagatg gtgacgcaag atgcctcggc gatttttgcc aaagttcatc
     8881 cagatgacat tcaagccatc actgattcaa tccaagaatc ggcccgcaac ctgacaccct
     8941 ggcagcatga atttcgtgtt cggcatgatg atcgcacggt gcgctggctg tatggcaatt
     9001 ccttgcccga acgtctggaa gacggatcgg tgctgtggca tggttttgtc actgacatca
     9061 ccgaacggaa agcgaccgaa gaccgcttgc gccagttgtc gcgctcggtc gagcaggccc
     9121 cggtgacgat tgtcatcacc gacctgaaag ggagcatttt gtatgccaat cccagtttta
     9181 ccaggtccac tggctacagc ctggccgagg cgtatggcaa aaatccgcgc attctcaagt
     9241 ctggcctgac cccgccggag gtctatcggg tgttgtggga aaccttgatc gctggtggtg
     9301 tctggcaggg tgaactgcat aaccgcaaaa aaaatggcga tctgtacatt gaaaacgcga
     9361 tcatcgcacc agtgcaggat gccaacggca tgcccagcca ctatgtggcc atcaaggaag
     9421 acattaccca gcgcaagcag gccgagcagg cgtttcaggc aacgctgcag gaaaaagttg
     9481 cgcttctgca tgaggtgcac caccgggtca agaacaacct gcaagtgatc tccagcctgt
     9541 tgcgtctgga aaccggacgc agcattgagc cggaaacccg cgccgtactg aaggaaatgc
     9601 agggccgcat tcttgccatg gcgctgctgc acgaaacgct ctaccgggtc ggcagctttg
     9661 cctcggttga actgggtgac tacctgaagc aattggccag ccaggcgttt cgggcgcaaa
     9721 gcagtcaagg catgctggtg cggctggtgc tggacctggc ggcggtgcag gtggtgatgg
     9781 atcaggccac accctgcggt ctgctggtca atgagctgat ttcaaatagc ctcaaacatg
     9841 cttttccagc aggtcgcagc gggcaggtat cgattggctt gcaagccgtg ccagcgcctg
     9901 acggcaaccc cgacctgctg cgcctgtggg taagcgacac cggcgttgga ctgccgccgg
     9961 actttgaggc tcgctgccgt cagtcgcttg gcatgcaact ggtgaccgac ctggcccgcc
    10021 agctcggcgg tgcgctgcag atcggccctg gccccggcag cgagtttgcc gtgacctttg
    10081 tccggcaaag ctgaaggcac cagcagcgaa tctgacccat cgctggacgc ggactcaagc
    10141 ttgctgcgtg aaccgctcga actctgccag ggccagcggc cgactgtaga gatagccctg
    10201 gtaggcatga cagcccaggt gcgccagaaa gtccttttgt gcctcaatct ccaccccttc
    10261 ggcgatcact gacaggccca tgctctcggc cagggccacc accatcttgg caatcgctgc
    10321 gtcattgggg tcggtcagga tgttgcggac aaaaccctgg tcaatcttga gctggtccag
    10381 cggcagccgc ttgaggtagc tcaacgacga gtagccggtg ccaaaatcgt cgagtgaaaa
    10441 gccaacgcct gaggccttca gggatgtcat cttggtgatg atgccctcga catcattgat
    10501 catcaggctc tcggtcagct ccagtttgag tcgcttcggg ttgacgccgg ttcggaccag
    10561 aacactttgc accctggcga caaaatcagg ctggtgaaac tgcttggcgc tgacattgac
    10621 ggccagcgtc aagtgggcca ggtcggggcg tgcgctccag gtcaccagtt ggcggcaggc
    10681 ggtctccagc acccactggc ccagcggcag gatcaagccg gtctcttcgg ccagaggaat
    10741 gaactcggcc ggcgacacca ggccgcgctc ggggtgccgc cagcgcacca gcacctcggc
    10801 accggtcagg cggccatcgc ccaccacctg cgcctggtaa tgcagcaaca gttggtcttg
    10861 cagcaaggcc tcggacaaat cggattccag ggcggcacgc tgcgtcacca ccacctgcat
    10921 ttgtgcgtca aaaaagcgca gcgcgttgcg cccggctgcc ttggcctggt acatcgccag
    10981 ttcagcacgc tttaatggct catcaatcgt ctcgtgcggc gcttcgccaa acagcgtgat
    11041 gccaatgctg ggtgtgctgt gatgctcgta agagccgatg gtgtagcttc ggttaaggct
    11101 gagtcgaatt ttctcagcca cggcttcaga ctgagttgcc gcatcaaccg cgttgtcgct
    11161 caggtgttcg atcatcacca caaattcgtc gccgcccagc cgtgccacgg tgtcaccctc
    11221 acgcacacag gcactcagcc gacgggccac gctttccagc agcatgtcac cctggaaatg
    11281 gccgagtgtt tcgttaaggg tcttgaagtt gtccagatcg acaaacagca gcgcaccgca
    11341 ggaccggtgg cggctacaag tgatcagcgc ctgtgccagc cggtccagca acaggcggcg
    11401 gttgggcaac tgcgtcagtg ggtcataaaa ggccaggttg ttgatttgct cttcggcaga
    11461 tttgcgggcg ctgatgtcaa aaaaagtggc cacgtaatgg ccaacctgac cggcagagtc
    11521 cttgacggca ctgattgaca gccattccgg gtagatttcg ccgtccttgc gccggttcca
    11581 gatttcgcct tgccagttgc cctttatggt cagacttttc cacatcatct ggtaaaaaac
    11641 cgcgtcgtga cgacccgagg ccagcagcct cgggttctgg cccagtgctt cttctgcgct
    11701 gtagccggta atgcgggtga aggcctggtt ggtgcgcagg atcgtgcctt gcgcattggt
    11761 gatggtgatg ccctgctgcg actcgaatgc ggtggctgcg atgctcagct ctgcctgggt
    11821 tcgcttgatt gccgtgatgt cgcgaatgat cgaatagttg gtgcgctggc cgtcataaat
    11881 gattggcgtg gcttgcactt cgacatcaat gatcgatcca tcgagcttga caaaccgggt
    11941 ctcggcgcgt ggcaggctgc cgccttgttc attttgccgc tgcatgcgct gacgcacaaa
    12001 agcctgcgcc tcgggtgcca ccagatcaat caagttgcga cccaccaggt cggacatggc
    12061 gttggcacca aacagtgcga tggccgctcc attggcatag accagcaccc cgtcacgatg
    12121 taccagcgca gcgtcgggcg accactcgat cagggtgctg tagcggttct cgctttcttt
    12181 cagggctgta tggacttttt tcccttcggt gatgtcgttg atgacgcaga cgataaagcg
    12241 ccccttgcca ctcgcatcgt caaaactgcg gcaggtcaga tggccccaaa attcactttg
    12301 gtcggctcgg cagtaaatcc ggtccacctc cgagctgttg atggtgttgg ccaacagcgc
    12361 ctgcatgttt ttgtagcccg cgtcttgcaa gctcggatgc agcagcgata catagtcgcg
    12421 cccattcagg gcctcggcgc tgcagccaaa catgtcggac atgcgccggt tcacgtggtt
    12481 gatgcgtccc tcggcatcga tcaggtagat tgccacgctg gaggtgtcaa gaatctgttg
    12541 cagaaacagc tcccggtgtt tcaacgtcgc caatagccgg ttgaagctgc caatcagctt
    12601 gccaatttca tcttggcggg caattggcaa gggctgcggc gcttgatccg aatcggccag
    12661 attggccagc gtttgcaccg ccgaaaccat cggcgagagt tctcgcttga gcagccacca
    12721 gatcagcgtg cccgctacca gcgtgaacaa cagcgtcgcc agcaatgtgc gtgtttgcaa
    12781 tcggacgaat ggcgcaaagg cctccgccgt gggcagtagc acggccaact gccagcccgc
    12841 cgttggcact cccttggcag cagccagcac ttgctgcccg tttgcgccaa ctgccacaga
    12901 aaacccctca aacccaagca acagccggtc catttccgga ttgatgcccg gcatcggcag
    12961 ttgctccagc acccggcgct tgtcggtggc cgtgacgacc agccgactgc gcggtgcgat
    13021 cagcacaaaa ctgccgctgg ctccgtagct gttacccacg tacttgtcga gaaaattcac
    13081 cccgctcaaa tcggttgccc cggacagcgc cccaatgacc tgacctgccg ggtcaagcac
    13141 cggaacactc atgacaaatg cggcaaaccg gttgggccac gacaagtggg gctggccgac
    13201 aaccgctttg gctcccttga agacatcaat caggtcttcg cgcacaacat cgctatggtt
    13261 ggggctgccg ctggccgatg ggatggctgt tatggcgctg ccatcgggct ggtaaaccgc
    13321 gataccattg ttgaaccggg aaaccaaatc ttgccgctgc gtcaaatgct gtcccagggc
    13381 caccgggttg gcgatcaggg ctggtgtcag ggtggcagcg gcaaactgca gtgccgccag
    13441 gtgcctggca atttcccgat ccatgtcggc agccaccaat gtgaccatcg agaactgctg
    13501 attgccttgc aaactctcca ggtcctcgcg cagcatgctg ctgacgtacc aggccagcga
    13561 caccatgccc agcagcacaa tgcccagcgt catcatcgtc acgcgggtct tgagagatcg
    13621 ccagcagaac cagtaaatct tcataaaaaa ggcttgtacc ccttgtccat attgcgcaag
    13681 tagcttctct aaatatagca caaagaaacc ggaaggttcg ttgcctggcc gcctcaggca
    13741 aaactggagc cgagcttgaa caccaccacc agccgcacca gatcattcgc ttgtgccttc
    13801 aaactgccag cgccagccac catgtcttga gccagcgcag cgcgaatgtc agcagcacct
    13861 tcacggttgc agcgagtcga agaatggcag gttcaaaatg tctcccaatc ggcatcgttg
    13921 ccgtctgcac tggctggctt gacagccttc gctggcggca aggcacgcgc agcaggcaca
    13981 cccgatggcc gtagcaccgc aggcctggcc gggcgggctg ggctgattga ccggcgcttg
    14041 ctggctcgca tggcgctggg cagatgttgg gccgtgttgt ggctgtcgag cttgaacacc
    14101 gccaccacct gcaccagatc atgcgcctgt gccttcaggc tgccggccgc tgccgccatt
    14161 tgctcaacca gtgccgcgtt ttgctgcgtc atcttgtcca tttcagtcac cgcactgcca
    14221 acttgtgaca ccccggcgct ttgcgcgcca ctggcggcac tgatctcgcc catgatgtcg
    14281 gtgacgcggc ggatgctgct gaccacctcg ttcatggtgc tgccggcctg gtcaaccagc
    14341 gcgctaccct gttcaacccg cgtcacgctg tcgttgatga gcgccttgat ttccttggcg
    14401 gcatcggccg agcgcccggc caggctgcgc acctcgctgg ccaccacggc aaagccgcgt
    14461 ccctgctcgc cggcgcgggc ggcctcgacg gcagcgttga gcgccagtat gttggtctga
    14521 aaggcaatgc cgtcaatcac cccgatgatg tcggcaatct tgcgtgagct ggcgttgatg
    14581 cctttcatcg tgtccaccac ctgcgccact accgccccac cctgcaccgc gaccgtgctg
    14641 gcgctcagtg cgagttggtt ggcctgcacc gcgtgatcgg cattttgctt gacggtggtg
    14701 ctgagctgct ccatgctggc agcggtttgc tgcagcgcgc tggcctggct ttcggtgcgc
    14761 gacgacaggt cgttgttgcc ctgggcaatt tcagtgctgg cggtggccac accttccgag
    14821 ccctgccgca cctggcccac aatgcgggca aacttgtcac gcatcacggc cagcgcctga
    14881 agcaggctgg cgatttcggg gttcggcacg gcaatctcca ccgccaggtc gccgtcggca
    14941 atgcgccgtg tgatctcggc ggcataagcc ggttcaccgc cgagctggcg cagcacgctg
    15001 cgcgaaatca acacaccaag cccgagcatg acgagcacca gaatcatcgt gccaacggca
    15061 aagccaatcg cccgctgaac catcaccgca tcaaccgtgt caatgtaaac gcccgagcca
    15121 atgatccagc cccagggcgc aaaacctttg acgtaggata ttttgggcac cggattgggg
    15181 ctgccgggct tgggccacat gtagggcaca tagccgctgc cgctgctgct ggttttgacg
    15241 gtttcaacaa atgcgcggaa caaatatttg ccgtccgggt ccttgttgtc agtcaggtct
    15301 ttgccgtcga gttccggctt gatcgggtgc atcaccattt tgggctgcat gtcgttgatc
    15361 cagaaatact caccgttacc gtaacgcagg gcaccgatga ccgacatggc ccgctgtttg
    15421 gcatccgctt ctgacacctt gccgtcggcc accatttttt gcgagtgctt cagcatgtcg
    15481 tagccaattt ccaccaactg cttgacaccg ttttggcgct cttccagcac cagtcgccgc
    15541 tccgagacca agaaaatgct cgccagaata acgatgccca gcactgcgct aagcgtcagc
    15601 aagcccagtc gccgggcgac aggcatggaa gatagattga acattttgat tccttcgact
    15661 gattaacttg tttcggaaac gaaagcagct cactgacgac acaggctgca atcgaccgac
    15721 gcctcacttt gggagacttt tgacattcgc gtgaaaaacg gctgtagctt gtcaccaaaa
    15781 tctgcgcttt ggagtcgggg aaaccctacc ctatgtaaga aacatctgat tgttttcaga
    15841 gcaatctgca atcgttcaag cttgattcct cagttggaca cgcccaagtg ggccgctggc
    15901 ggcgcggctg ggtcgggcga atgccgcggc tccagacgtg cctgcgcacg tttggacggc
    15961 ttgaagcggg ccagcctagg atgtggtcgg gaataactcc ccgttttgca ccaaggagtt
    16021 gggatgcacg g
//
LOCUS       DAOSLL010000019        16018 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_3817_length_16018_cov_4.448475, whole genome shotgun sequence.
ACCESSION   DAOSLL010000019 DAOSLL010000000
VERSION     DAOSLL010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 16018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16018
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3817_length_16018_cov_4.448475"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PLB25_01495"
     CDS             complement(<1..522)
                     /locus_tag="PLB25_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOE40300.1"
                     /translation="MSAVSTSEKGTDYIIADIGLAAWGRKELNIAETEMPGLIAIREE
                     FSESKPLQGARITGSLHMTIQTAVLVETLQALGAQVRWASCNIFSTQDHAAAALVAQG
                     TPVFAYKGESLRDYWDYTHRIFEFGGKKGTAAEGPNMILDDGGDATLLMHLGTRAEKD
                     SSLLDKPTSEEETC"
     regulatory      complement(596..707)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            882..3314
                     /locus_tag="PLB25_01500"
     CDS             882..3314
                     /locus_tag="PLB25_01500"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HOE40301.1"
                     /translation="MNTKHLPTETGESIDAHLLHTIGVDPANASQSDLMLAVAQMARS
                     QLSERWVETQAQERKLKARRVVYLSMEFLMGRTMSNALAALDLTDVARNGLAQHAQKL
                     EDVADREHDAALGNGGLGRLAACFLDSMATLGLPSWGYGIRYEYGMFAQHIAGGTQQE
                     YPDSWLVNGTPWEFPRADISYPVRFGGWVEHLDGQAYWRNSGEVAAKAYDMVVPGHGT
                     TKVSTLRLWKAVAPAHFDLGAFNTGDYARAASAKNEFENISWVLYPNDSTPAGRELRL
                     KQEYFFVAASMQDLVKRHLDEHPSLANLAEHVAIHLNDTHPAIGVAELMRILCDEHNM
                     PWADAWQLCCKTFSYTNHTLMPEALETWPVGLMQYVLPRHLEIIFRINKEFLEFAAKK
                     RPNDNQFLSRLSLIDEHGDRRVRMAHLSIVGSHTVNGVSALHSDLLVQTIFSDFAYLW
                     PERFTNMTNGVTPRRWLAQANPGLAALLDETLGTGWRLDLDQLKRLDDKKSDPAFQQA
                     FMAVKRANKVRLAATIAHTTGIEVSPDSLFDVQVKRIHEYKRQLLNVLHVVWRYQAIL
                     AEPNAHWVPRTVIFAGKAASSYVSAKSIIRLIHDVGQIVNNDPRIGDKLKIVFIPNYG
                     VSVAEVIMPGADLSEQISTAGTEASGTGNMKLALNGALTIGTDDGANIEIRQNVGDDN
                     IFIFGLKTPEVKSLRQAGYQPMRFYEKQPALKSVLDAISSGRFSPDEPARYRPLMDSL
                     LWGGDHYLLLADFESYITAQARVDAVYRHPAQWCQHSIANVAGMGVFSSDRTITEYAK
                     TIWHVAPVASGQ"
     gene            3355..5532
                     /gene="glgB"
                     /locus_tag="PLB25_01505"
     CDS             3355..5532
                     /gene="glgB"
                     /locus_tag="PLB25_01505"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HOE40302.1"
                     /translation="MLHTRYIEMICSASHGDPFAVLGPHLSSPGQTSIRCFLPGAAKV
                     TVLDGKGAALGDLSLRHIDGFYEETVKQAPDAPYRLRVKWESGIEDTFDDPYRFPLVL
                     GEMDVWLLGEGTHLRPFEVLGAQISTMLGIAGVRFAVWAPNASRVSVVGDFNLWDGRR
                     HPMRQRRECGVWELFLPGIGRGARYKFEIRSNSGHVLPQHADPYALQSELRPATASVV
                     CELPAVQPPSHERQLANALNAPISIYEVHLGSWRRVTEDGNRWLTWDELADTLVPYVQ
                     EMGFTHIELMPVSEHPFDGSWGYQPIGVYAPTSRFGEPAGFGRLVERCHAAGIGLILD
                     WVPAHFPTDIHGLANFDGTQLYEYADPKEGFHNDWNTLIYNLGRTEVRNFLVGNALYW
                     LERFGVDGLRVDAVASMLYKDYSRKAGEWVPNAYGGRENLDAIAFLKRMNEVVGVERP
                     QAITLAEESTAYPAVSRPTFAGGLGFHYKWNMGWMHDTLSYISRDPIYRQHHQGELTF
                     GLLYAFNENFVLPISHDEVVHGKGSLLNKMPGDRWQQMANMRVFLSYMFGHPGKKLLF
                     MGCEFAQEREWNHDHSLDWHLLEQPAHVGIQRLVRDLNRLYRATPALYQQDFVSAGFE
                     WIEHNDTQRSTVSFARRSESGSFMLLVCNFTPNVWYDYRMGVPQPGNYREALNSDSEC
                     YGGSNVRAITGQVTAEDIHWNGRAQSIALTLPPLAAVFIEWTS"
     gene            5520..7643
                     /gene="glgX"
                     /locus_tag="PLB25_01510"
     CDS             5520..7643
                     /gene="glgX"
                     /locus_tag="PLB25_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOE40303.1"
                     /translation="MDELSAATLQAGRAWPLGATYDGHGVNFAVFSANAQALDLCLFD
                     ASGNNELARLRLFCRNGDVWHGYLPGAQPGLVYGFRAHGPWRPDRGHRFDSSKLLLDP
                     YARDIVGEFIWRDEHFAPDRQHPRHLETHDNARLALKARVINDPFDWDDDQAPGIPLA
                     ASIIYECHVKGFTKRNPLIPPELRGTFAGMGHSASINHLKLLGITAVSLLPVHYSLSE
                     DRLIAMGLSNYWGYNTIGFFCVNPRLCSGQGGVSPRNEFRSMVRSLHAAGLEVILDVV
                     YNHTAEAGQSGPTISFRGLDNASYYRLPPNDLSRYENFSGCGNTLDVQQPRVLQLVMD
                     SLRYWVSEMHVDGFRFDLATVLGRSDSGFSPQAAFFAAVAQDPVLCRVKMIAEPWDIG
                     PGGYQVGQFPRGWLEWNDQFRDTVRRFWVQSAASPRSPSDGATRGDFAMRLCGSSDLY
                     QARRRPPAESLNYVISHDGFTLRDLLSYNQRNNLANGEDNRDGTGNNISFNCGVEGPS
                     DAPTVNRMRARLQRALLTTLLLGQGTPMICAGDELGHTQDGNNNPYCQDSPMTWINWQ
                     TVDTDLLAFTQYLISLRHQLRPFANHWYSGAADADGNYDLSWWNPDGSALQGDSWHQP
                     MQRALGCLIGKPGRAKMPLLLLINSGASPETFALPRGQWQALLDTSHPRGLAVWRAAG
                     TTPVPVVAHSLLLLQQTTTRPYHKA"
     gene            complement(7680..9188)
                     /gene="malQ"
                     /locus_tag="PLB25_01515"
     CDS             complement(7680..9188)
                     /gene="malQ"
                     /locus_tag="PLB25_01515"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOE40304.1"
                     /translation="MANPLLLQRRAGVVLHPTSLPGPHGSGDFGPNAFKFVDWLQAAG
                     QTLWQTLPLGPVGPGYSPYMGSSAFAGNPCLVAFEPMVELGWIDADSLMVKFDELKIN
                     YPNLVPWRMQKLREAFAGFIKRGNQREQRALYDWAQSQQAWLDDYTLFMALDNHYCPA
                     LWPDWPSNLAKREPMAMVNARLEFASELDFWCFVQWQFDVQWQAIKNYAHQKQVLLVG
                     DLPIFVAHHSADCWARPDLYVLDESGKPTVVAGVPPDFFSATGQRWGNPLYDWAAMQQ
                     DGYQWWIERVRRQLLLADIIRIDHFRGFVDYWEIPADEPTAICGRWQPGPGDDLFAAL
                     VGALGDLPIIAEDLGIITPEVTALRERIGFPGMKVLLFAFSGDESHVFLPENYESNTV
                     VYTGTHDNDTVQGWWATCTEHERAFAKRYLNTDGSDLHWAMLRAGSMSVANISLCQFQ
                     DVLGLDGTHRMNTPGTMGCWTWRFIWDWVEPPVTQRLAQLTADSGRCEAATT"
     gene            complement(9236..10699)
                     /gene="glgA"
                     /locus_tag="PLB25_01520"
     CDS             complement(9236..10699)
                     /gene="glgA"
                     /locus_tag="PLB25_01520"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HOE40305.1"
                     /translation="MKVLQVSAEIFPLIKTGGLADVAAALPVALNAAGCDTRLLLPGF
                     APIMADLRASKVLAELVAPWGERLVLRRGHLASLDLLAYVIDRPELYCRPGTPYEDLE
                     HQPFADNYRRFALLGWVASRLASGLDSDWRPQVVHSHDWHAALASAYVAFLPVQNFHV
                     ASVYTVHNLAYDGLFAPRHFFELGLPSGAFSVDGLEFHGQMSFMKAGLFFSDHITTVS
                     PSYALEIQTPEDGYGMDGLLRLRANALTGILNGVDESVWDPARDPAISHPFDAHNTEG
                     KQRCKAWLRQELGLAVQNDAPLFGIVSRITEQKGLHLVLNVLDELLAQGGQLVMMGEG
                     DPEMEAAFRQRALSSPQAVSVHIGYNERFAHRIFAATDVTLVPSSFEPCGLTQLYGLK
                     YGSLPLVHHVGGLADTVVDCTLEDLANETANGFVFYRFDAQGLRRAMSRAFALYARKA
                     EWRAVRKRAMSQPLGWDKAAAQYVNLYRKLCPEPFQN"
     gene            complement(10718..11989)
                     /gene="glgC"
                     /locus_tag="PLB25_01525"
     CDS             complement(10718..11989)
                     /gene="glgC"
                     /locus_tag="PLB25_01525"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOE40306.1"
                     /translation="MPAVDKIPTTHQLVRHTVALVLAGGRGSRLKQLTDSRAKPAVYF
                     GGKFRIVDFALSNCLNSGIRRMAVVTQYKSHSLMRHLQRGWNFLRAELNEMVDMLPAQ
                     QRAGEEHWYRGTADAIFQNIDIIQSSHPEYVVILAGDHVYKMDYSIMLKDHVVRGAGC
                     TVGCIEVPREEASAFGVMAVDAYRNITAFVEKPANPPTIPGNDAVSLASMGIYVFNAS
                     YLYKLLEEDLANPDSEHDFGKNVIPKAVADGHALAHPFGMSCVSRVDDAAPYWRDVGT
                     IDAFWSANLDLASTVPELDIYDIEWPIWTYQRQLPPAKFVPDTFGQNGVTVNTLISGG
                     CIVSGSYVAHSVLFSEVRVHSFCRVDQAVVLPDVTINRNCRLTKVVIDRGCDIPEGLV
                     VGEDPALDAQRFERTEGGVVLITREMIARLN"
     gene            complement(12065..13717)
                     /locus_tag="PLB25_01530"
     CDS             complement(12065..13717)
                     /locus_tag="PLB25_01530"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HOE40307.1"
                     /translation="MTIQTHPTTPLAGQRPGTSGLRKKVTVFMQPMYLENFVQSLFDV
                     LNASAADGQGLRGQTLVLGGDGRYHNRVAVQTILKLAAANGVQRVLLGRGGILSTPAV
                     SAVIRRRKAFGGIVLSASHNPGGPDGDFGIKYNASNGGPATETLTEAVYARTLTISEV
                     RASDAPDIAIDELGEQSVEQMQVEVIDSVADYAAVMRELFDFGMLRGMFARGFTMRVD
                     AMCAVGGPYAKAIIEGELGAPAGTVVNAEPLEDFGGLHPDPNPVNAEDLIAHMAQSDA
                     PDFGAAMDGDADRNMVVGRNFVVTPSDSLALMTAHARLVPGYKDGLLGVARSMPTSAA
                     VDRVAKKLGIACFETPTGWKFFGNLLDAGRVTLCGEESYGTGSSHVREKDGLWAILFW
                     MSLQGATGRSVESLVREHWAAYGRNYYTRHDYEGVAVDAANALMTGLRAALPGLPGQS
                     FGTRTVTLADDFSYADPVDGSVSNAQGIRLIFDDGARVIFRLSGTGTEGATLRLYLER
                     YEANPALQDVPVQQALQDLAALADQVAGIARHTGMSGPSVAT"
     gene            13903..15534
                     /locus_tag="PLB25_01535"
     CDS             13903..15534
                     /locus_tag="PLB25_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HOE40308.1"
                     /translation="MTYAFETRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQIAGSVK
                     ARKATRHATSDWMEIEKQRGISVASSVMQMVYRDHVINLLDTPGHKDFSEDTYRVLTA
                     VDSALMVIDAANGVESQTRRLIEVCRQRDTPIITFINKMDREVRDPLDILDEVERELG
                     MPCVPMTWPVGQGKTFGGIINLRTRGMTVFEAGSERRPQDFETMPLTDTEALTKRFGF
                     EIDTAFESMELATGASPSFDREAFLAGQQTPVFFGSGVNNFGVMEVLDALIDLAPSPG
                     PRTSSFVVNKQPVIRQVQPEDEPFSGVVFKVQANMDANHRDRIAFVRMASGKYTPGLK
                     LKVMRTGKELRPTSVVTFMSQRREAVEEAYAGDIVGFTTHGGVQLGDTITDGSVNLLF
                     TGLPFFAPEMFMTVVLKNPLRTKQLQQGLAQLGEEGAIQVFRPELGGNMLLGAVGQLQ
                     FEVVQHRLKGEYDADIRLEGSQYTGARWITADSATELKDFVHAYPQRMARDAADTLAY
                     LCTSPYDVRLAQERFPKIHFHPLREHAGLALQSAN"
     gene            15548..>16018
                     /locus_tag="PLB25_01540"
     CDS             15548..>16018
                     /locus_tag="PLB25_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOE40309.1"
                     /translation="MLALSQWPLQARRQIIGVFTDIDDTLTTHQAITPEALQALVDLK
                     AAGLAVIPITGRPVGWCAPHAQGDAVQGVLPWSVDAMVAENGGVAFVPENNGQNSLQP
                     LCSKRYKLSKIYYSDAAARAASQLRMQQVAQRILQQVPGATLSRDSAGRETDLAI"
BASE COUNT         3490 a         4732 c         4434 g         3362 t
ORIGIN      
        1 acaggtttct tcttcgctgg tcggcttgtc gagcaggctg ctgtcctttt cggccctggt
       61 gcccaggtgc atcagcagcg tggcatcacc accgtcgtcc agaatcatgt tcgggccttc
      121 ggcggctgtg ccttttttgc caccaaattc aaaaatgcgg tgtgtgtagt cccaataatc
      181 ccgcaaggac tcacccttgt aagcaaacac cggtgtgccc tgtgccacca gagccgctgc
      241 ggcatggtct tgtgtcgaga aaatattgca cgacgcccag cgcacctgtg cgcccagcgc
      301 ctgcagggtt tccaccagca cggcggtctg aatggtcatg tgcaaggacc cagtgatgcg
      361 ggcaccttgc agcggctttg actcggaaaa ctcttcccgg atggctatca ggccaggcat
      421 ttcggtctcg gctatgttga gttccttgcg accccaggca gccaggccga tatcggcaat
      481 gatgtagtcg gtgccttttt cagatgttga tacagcgctc atgattcact ccagcagttg
      541 atgaatgaca ctgcggcgac aacaagctgc aaaaaaaagc tcaccgcacg ccatcagtgg
      601 gtgagcgccg ttggatgcgc cgcaggcatg catgcaagcg gattccgagc ctcacgcgac
      661 acctgaccga catggtcatg gtgcgcgctg caacgctcct cggatggcaa aaattatagg
      721 acacgatcat cttcggacaa ttcctacacc tgtttcgccc tttttggagt tgcagtcggg
      781 cttttgtgac tattcagtaa cagacccgca acatcgctaa gatcaggggt gcaaaataca
      841 cgctgtgcca tataaatttg acaacatcca aaggaatgcc cttgaatacc aagcacttgc
      901 ccactgaaac cggcgaatca atagatgctc acctgttgca caccattggt gtcgatcctg
      961 ccaacgcttc gcaaagcgac ttgatgctgg cggtcgcgca aatggcacgc tcccaattgt
     1021 cggagcgctg ggtcgagacc caggcgcaag agcgcaaact caaggctcgc cgggtcgtct
     1081 acctgtcgat ggaattcctg atgggacgca ccatgtccaa tgcgctcgct gcactcgacc
     1141 tcaccgatgt ggccagaaac ggattggcgc agcacgcgca aaagcttgag gacgtggccg
     1201 accgtgaaca cgatgcggcg ctgggcaatg gtggcctggg tcggctggcg gcctgttttc
     1261 tggactccat ggcgaccctt ggcctgccct cctggggata tggcatccgc tacgaatacg
     1321 gcatgtttgc ccagcacatt gccggcggca cccagcagga gtatccggat tcatggttgg
     1381 tcaatggaac accctgggag tttccacgtg cagacatcag ttatccggtg cgctttggcg
     1441 gctgggttga acatcttgac ggtcaagcct attggcgcaa ttcgggtgaa gtggccgcca
     1501 aggcctatga catggtggtg ccaggccacg gcacgacaaa ggtcagcacg ttgcgactct
     1561 ggaaagcggt agcaccggcg cactttgacc tgggtgcctt caacacaggt gactatgcgc
     1621 gcgctgccag cgccaaaaac gagttcgaaa acatctcctg ggtgttgtat cccaacgaca
     1681 gtacccctgc cgggcgcgaa ctgcggctca agcaggagta ttttttcgtg gccgcctcga
     1741 tgcaggatct ggtcaagcgc caccttgacg agcatccgtc gctggcaaac ttggccgagc
     1801 atgttgcgat tcacctcaac gacacccacc cggctatcgg ggtggccgaa ttgatgcgca
     1861 ttttgtgcga cgaacacaac atgccttggg ctgatgcctg gcaactgtgc tgcaagacgt
     1921 tctcgtacac caaccacacc ttgatgcctg aagcacttga gacctggccg gtgggtttga
     1981 tgcaatatgt gctgcctcgg catctggaga tcatctttcg catcaacaag gaatttttgg
     2041 aatttgccgc caaaaagcgt cccaatgaca accagtttct gtcacgcttg tccctgattg
     2101 acgaacacgg cgaccgccgg gtgcgtatgg ctcacctgtc gattgtgggc agccatactg
     2161 tcaatggcgt gtcagcgctg cactccgatt tactggtgca aaccattttt tccgactttg
     2221 cctacctgtg gccagaacgt ttcaccaaca tgaccaatgg tgtcacgcca aggcgctggc
     2281 tggcccaggc caaccccggg cttgcggcac tgctcgacga aacactgggc actggctggc
     2341 ggcttgacct tgaccagctc aagcggctcg atgataaaaa gtccgatcct gcattccagc
     2401 aagcctttat ggccgtcaaa cgcgccaaca aggtccggtt ggcggcaacc attgcccaca
     2461 ccaccggcat tgaggttagc ccagacagcc tgtttgatgt gcaagtcaag cgcattcacg
     2521 agtacaaacg gcagttgctc aacgtgctgc acgtggtgtg gcgctaccag gctatcctgg
     2581 ccgagccgaa cgcccactgg gtgccgcgca cagtcatttt cgccggcaag gcggcttcga
     2641 gttatgtcag cgccaaatcc atcattcgcc tgattcatga tgtcggccag attgtcaaca
     2701 acgatccgcg cattggtgac aagctcaaaa tcgttttcat cccgaattac ggcgtatcgg
     2761 tcgctgaggt catcatgccg ggtgctgact tgtccgaaca gatttcaact gctggtaccg
     2821 aagcctcagg caccggcaac atgaaacttg cactcaatgg cgcgttgacc attggcaccg
     2881 atgacggtgc caatatcgaa attcgccaga atgtgggtga cgataacatc ttcatctttg
     2941 ggttgaaaac ccccgaggtc aagtcgttgc gccaagccgg ttaccagccg atgcgttttt
     3001 acgagaagca acctgcgctc aaatctgtgc tggatgccat ttccagtggt cgcttttcgc
     3061 ctgacgagcc ggctcgttat cgccccctga tggattcact gctctggggt ggtgaccatt
     3121 acctgttgct ggctgacttt gaatcctata tcaccgcaca agccagggtt gatgcggttt
     3181 atcgacatcc tgcccaatgg tgtcagcatt ccatcgccaa cgtggccggc atgggtgtct
     3241 tttcttctga ccgcacgatc accgagtatg ccaaaaccat ctggcacgtc gctccggtgg
     3301 catccgggca gtaaaattgg tgcacatcat cacctgacac tttgactgaa tctcatgtta
     3361 cacacccgct acattgaaat gatttgcagc gccagccatg gcgatccctt tgcagttctt
     3421 gggccgcatc tcagttcacc aggacaaacc tcgatccgtt gctttttacc cggagccgcc
     3481 aaagtgaccg tattggatgg caagggggca gctttgggcg acctcagttt gcgtcacatt
     3541 gatggtttct atgaagaaac ggtcaagcag gcccctgacg cgccctatcg cctgcgggtc
     3601 aaatgggaaa gcgggatcga agacacgttt gacgaccctt accgctttcc attggttctg
     3661 ggcgaaatgg atgtctggtt gctcggcgaa ggcactcact tgcgtccttt tgaagtgttg
     3721 ggtgcgcaga tcagcacaat gctcggcatt gccggtgttc gttttgcggt gtgggcgccc
     3781 aatgcctccc gcgtcagcgt agtgggtgat ttcaaccttt gggatggccg acgtcacccg
     3841 atgcgtcagc gtcgtgaatg tggtgtatgg gaactctttt tgcccggcat tgggcgtggt
     3901 gcgcgttaca aattcgagat tcgcagtaac agtggtcatg tgctgcccca gcacgctgat
     3961 ccctatgcgt tgcagtcgga actgcgcccc gccacggcca gtgtggtgtg tgagctgcca
     4021 gccgtacagc cgccgtcaca tgaacgccaa ctggccaatg cattgaacgc tccaatcagc
     4081 atttacgagg tgcatctggg ctcctggcgg cgcgtgaccg aagacggtaa ccggtggctg
     4141 acctgggacg agttggccga cacgctggtg ccatatgttc aagagatggg ttttacgcat
     4201 atcgaactta tgccagtcag cgagcacccg tttgacggct cgtggggata ccagccgatt
     4261 ggtgtttacg cacctacttc gcgctttggc gagccggccg gctttgggcg gttggtggag
     4321 cgctgccacg ctgcaggcat cgggttgatt ctggactggg tgccggcgca ttttccaacc
     4381 gacatacacg gccttgccaa ctttgatggc acccagttgt acgagtatgc tgaccccaaa
     4441 gagggttttc acaatgactg gaacaccctg atttacaacc tgggtcgtac cgaggtgcgc
     4501 aactttttgg ttggcaatgc actttactgg ctggagcggt ttggcgttga tggcttgcgc
     4561 gtggatgccg tggcctcaat gctgtacaag gactacagcc gaaaggcggg tgaatgggtt
     4621 cccaatgcct atggtggtcg cgaaaacctg gatgccattg catttctgaa gcgcatgaat
     4681 gaagtcgttg gtgtcgagcg gcctcaagcc atcacgctgg ccgaggaatc tacggcctac
     4741 cctgccgttt cacgtccgac ttttgctggt ggtctgggct ttcactacaa atggaacatg
     4801 ggctggatgc atgacaccct gtcctacatt tcgcgtgacc cgatctatcg ccagcatcat
     4861 cagggtgaac tgacgtttgg tttgctctat gcgttcaacg agaactttgt gctgccgatc
     4921 tcgcacgatg aggtcgtcca tggcaagggc tccctgctga acaaaatgcc tggagatcgc
     4981 tggcaacaga tggccaacat gcgcgtgttc ctaagctaca tgtttggtca cccgggtaag
     5041 aagctgcttt tcatgggttg cgaatttgca caggaacgcg agtggaacca tgatcacagt
     5101 cttgactggc atttgctgga acaacccgcc cacgttggta ttcagcgcct ggtgcgtgac
     5161 ctcaatcggc tttatcgggc cactccggcc ctctatcagc aagactttgt atctgccgga
     5221 tttgagtgga tcgaacacaa cgacactcaa aggtcgactg tgagttttgc caggcgctcc
     5281 gaaagtggct cttttatgtt gctggtctgc aacttcacgc ctaacgtctg gtacgactac
     5341 cgcatgggcg ttccacagcc ggggaattac cgggaggccc tcaactctga ctcagaatgt
     5401 tacggcggaa gcaatgttcg ggctatcaca ggccaggtta ctgctgaaga tattcactgg
     5461 aatggcagag cacagtcaat tgcgctgacc ttgcctcctc ttgccgcagt cttcatcgaa
     5521 tggacgagct aagcgcagcg acactgcagg ctggtcgtgc atggccgttg ggtgccactt
     5581 acgacggtca tggtgtcaac tttgctgtgt tttctgccaa cgcacaagca ctggatttgt
     5641 gcttgtttga tgccagcggc aacaatgagc tggccagatt gcgactattc tgtcgcaacg
     5701 gagatgtctg gcacggctat ttgcctggtg cccagccagg cctagtttat ggctttcggg
     5761 cgcacgggcc ttggcggcca gatcgcgggc atcgctttga ctcatcgaaa ctgttgttgg
     5821 atccctacgc ccgcgatatt gtcggggagt tcatttggcg cgacgaacat ttcgcacccg
     5881 accgtcagca cccgcgtcac ttggaaaccc atgacaatgc ccggctggcg ctgaaggcac
     5941 gtgtcatcaa cgatcctttc gactgggacg atgaccaagc accggggata ccgctggctg
     6001 catcgatcat ttatgaatgc catgtcaagg gctttacaaa acgcaaccca ctcatcccgc
     6061 cagagttgcg tggcaccttt gccggcatgg gccacagcgc atcgatcaat cacctgaaat
     6121 tgctgggtat cactgctgtc agcctgctgc cggtccacta cagccttagt gaagatcggc
     6181 tcatcgctat gggtttgagc aattactggg gctataacac gattggtttt ttctgtgtta
     6241 acccgcgcct gtgcagcggt caaggcggtg tgtcgccaag aaatgagttt cgcagcatgg
     6301 ttcgctcgct gcacgcggcc gggctggagg tcattctcga tgtggtttat aaccatacgg
     6361 cagaggctgg tcagtctggc ccaaccatca gtttccgcgg actcgacaac gccagctact
     6421 atcggctgcc acccaatgac ctgagtcgct atgagaactt cagcggctgt ggaaataccc
     6481 ttgacgtgca gcagcccagg gtattgcaat tggtgatgga cagcctgcgc tattgggtca
     6541 gcgaaatgca tgtcgatgga ttccgctttg atctggccac ggtgctgggc cggtcagaca
     6601 gcggctttag cccccaagcc gcgttttttg cggcggtcgc gcaagacccg gtactgtgcc
     6661 gagtcaagat gatcgccgaa ccttgggaca tcgggccggg tggttaccag gtcgggcagt
     6721 ttccgcgcgg ttggctggaa tggaacgacc agtttcgtga tacggttcgt cgcttttggg
     6781 tacaaagtgc tgcctctcca aggtctcctt ccgatggcgc tacccggggc gactttgcca
     6841 tgcgcctttg cggctcttct gatctgtacc aggcgcgccg acgccctccc gccgagtccc
     6901 tcaactatgt gatctcacac gatggcttca ccttgcgcga tttgctcagt tataaccagc
     6961 gcaacaacct tgccaatggc gaagataacc gcgatggaac cggcaataac atcagcttca
     7021 actgcggtgt cgagggcccc agcgacgcac cgacggtcaa ccggatgcgc gccagacttc
     7081 agcgcgcctt gctgactacg ttgttgctgg gacagggtac ccccatgatc tgcgctggcg
     7141 acgaattggg acatacccag gacggtaaca acaaccccta ctgtcaagat agcccaatga
     7201 cctggatcaa ctggcagacc gtcgataccg atctgctggc atttacccag tacctgattt
     7261 ccttgcgaca ccaattgcga ccttttgcca accattggta cagcggcgct gcagatgccg
     7321 acggcaacta tgacttgtcg tggtggaacc cagatggtag cgcactgcag ggagattcct
     7381 ggcaccaacc gatgcaacgt gcactgggtt gcttgatcgg aaagccaggt cgggccaaga
     7441 tgccgctgct gttgttgatc aacagtggtg ccagcccgga aaccttcgcc ctgcccagag
     7501 gccagtggca agcactgctt gacaccagcc atccgcgcgg gctggcagtc tggcgggcgg
     7561 cgggtacgac accggttccg gtcgttgcgc acagcctatt gctgcttcag caaacgacca
     7621 cgcgccccta ccacaaagct tgatttaggc gctggtcgac agtatcttgg tgcgaccaat
     7681 caggtcgttg ccgcttcaca cctgccactg tctgccgtca gttgggccaa ccgctgtgtc
     7741 accggtggct cgacccagtc ccagatgaaa cgccaggtcc agcagcccat ggtgcccggg
     7801 gtgttcatgc gatgtgtgcc gtccagaccc agcacatctt gaaactggca cagcgatatg
     7861 ttggccaccg acatggagcc agcgcgaagc atggcccaat gcaaatctga tccatcggtg
     7921 ttcaggtagc gtttggcaaa agcccgttca tgttcggtac aggtggccca ccatccctgc
     7981 actgtgtcgt tatcgtgcgt gccggtgtag accacggtgt tggactcata gttttccggc
     8041 agaaagacat ggctttcatc gcctgaaaac gcaaacagaa gaactttcat tccaggaaac
     8101 ccgatacgct cgcgcagggc cgtgacctcg ggcgtgatga tgccgaggtc ttctgcaatg
     8161 attggcaagt cgcccaaagc accgaccaac gccgcaaaca ggtcatcccc agggccaggt
     8221 tgccaacggc cacatatagc cgttggctcg tctgccggaa tttcccaata gtcgacaaag
     8281 ccccggaaat ggtcgattcg gataatgtca gccagcaaca attgccgcct tacccgctca
     8341 atccaccact ggtatccatc ttgctgcatg gccgcccagt cgtacaacgg attgccccag
     8401 cgctgccctg tggcagaaaa gaaatcgggt ggtacccctg ccaccacagt cggtttacct
     8461 gactcgtcaa gcacatacaa gtcgggtctg gcccaacagt cagcactgtg atgcgctaca
     8521 aaaatgggta ggtcaccgac cagcaacacc tgcttctgat gggcataatt cttgatcgcc
     8581 tgccactgaa catcgaactg ccactggaca aagcaccaaa aatccagctc tgaagcaaac
     8641 tccaggcgag cattgaccat tgccatcggc tcccgcttgg ccagattgga cggccaatct
     8701 ggccaaagag ccgggcagta atgattgtcg agagccatga acagggtgta gtcatcgagc
     8761 caggcctgtt gcgactgcgc ccagtcgtag agtgcccgtt gctcacgctg gttaccacgt
     8821 ttgatgaacc cggcaaacgc ctcacgcagc ttttgcatgc gccagggaac caggttcggg
     8881 taattgatct tcaactcatc gaacttgacc atcaagctgt cagcgtcaat ccagcccaac
     8941 tcgaccattg gctcgaacgc caccagacag ggatttcccg caaacgccga gctgcccatg
     9001 taaggcgaat aaccagggcc gaccggtccg agtggcaagg tttgccaaag ggtttgcccg
     9061 gcagcctgaa gccagtccac aaacttgaag gcattggggc caaagtcacc ggacccgtgc
     9121 ggaccgggca aagacgttgg atgcaacacc acgccagcgc ggcgctgcaa caacaatgga
     9181 ttggccataa attaaatgct cctagtgcat gtcagggtct gccaccatgg cgacctcaat
     9241 tttggaacgg ctcggggcaa agcttgcgat acaaattcac gtactgcgca gcggccttat
     9301 cccaacccag cggctggctc atggcacgtt tgcgcacggc tcgccactcg gcttttcgag
     9361 catacagcgc gaacgccctg ctcatggcac ggcgcaatcc ttgcgcgtca aaacgataaa
     9421 agacaaaacc gttggcagtt tcattggcga ggtcttccag agtgcaatcg accacggtgt
     9481 cagccaaacc cccgacatgg tgcaccaaag gaagactgcc gtacttcagg ccatacagtt
     9541 gcgtcaggcc acagggctca aagctggacg gcaccagagt cacatcggta gcggcaaaga
     9601 tccgatgtgc aaagcgctcg ttataaccaa tatggaccga taccgcctgc ggactggata
     9661 gcgcacgttg tcggaaagcg gcctccattt ccggatcccc ctcacccatc atcaccaact
     9721 gaccaccttg ggcgagcaac tcatcaagta cattgagcac caggtgcaag cctttctgtt
     9781 ctgtgatgcg actcacaata ccaaacaaag gggcatcgtt ttgcaccgcc aggccaagct
     9841 cttgtcgcaa ccaggctttg cagcgctgct tgccctctgt attgtgggca tcaaatgggt
     9901 gggaaatagc cgggtctcgc gcagggtccc agaccgattc atcaacgccg ttcagaatgc
     9961 cggtcagagc gttggcgcgc aagcgcagca gcccatccat cccataaccg tcttcgggcg
    10021 tctgaatttc aagggcataa ctcgggctca cagtggtgat atggtctgaa aaaaacaagc
    10081 cggccttcat gaatgacatc tgaccgtgaa actcaaggcc atcaactgaa aatgcgcccg
    10141 acggcaagcc aagctcgaaa aaatgccggg gtgcaaacag tccgtcataa gccaaattgt
    10201 gtaccgtata aacactggca acatggaagt tctgaaccgg caaaaatgcc acataagcag
    10261 atgccaatgc ggcatgccag tcatggctat gcacaacttg tggccgccaa tcgctgtcga
    10321 ggccactggc caagcgggaa gccacccagc ctaacaacgc gaatcgacga tagttgtccg
    10381 caaatggttg gtgttcaagg tcttcataag gtgttccggg tcggcagtac aactcaggcc
    10441 gatcgatcac ataggccagc agatccagac tggcaaggtg accccgacgc aatacaaggc
    10501 gttcacccca aggtgctacc agctcggcca acaccttgct agcgcgcaaa tcggccatga
    10561 tgggggcaaa acccggaagc aacaggcgcg tatcacaccc tgcagcgttc agtgcgaccg
    10621 gcaaagctgc agcaacgtcg gctaagcctc cagttttgat gagtggaaaa atttctgcgc
    10681 tgacctgcaa gactttcata agcggcgagt tacttcttta gttcaatctg gcaatcatct
    10741 cacgcgtaat cagcaccaca ccgccttcgg tgcgctcaaa gcgctgagcg tccagcgctg
    10801 ggtcttcacc gactaccaga ccttccggga tgtcacaacc ccggtcaatc acaaccttgg
    10861 tcaagcggca gttgcgattg atcgtcacat cgggcagcac caccgcctgg tcaacccgac
    10921 agaaagaatg cactcgcact tcagagaaaa gcaccgaatg cgctacatag gaacccgaca
    10981 cgatgcagcc gccggaaata agggtgttga cagtcacccc attctgacca aacgtatcgg
    11041 gcacaaactt agccggtggc aactgacgct gataggtcca gatcggccat tcaatatcgt
    11101 aaatatcgag ctccggtacg gttgatgcca gatccagatt ggccgaccaa aaggcatcta
    11161 tggtccctac atcgcgccaa tagggtgccg catcatcaac cctggagacg cacgacatgc
    11221 caaaaggatg ggccagcgca tgaccatcag cgaccgcttt gggaatcacg tttttgccaa
    11281 agtcatgctc tgaatcgggg tttgccaggt cttcttcgag cagtttgtac agataactgg
    11341 cattgaacac atagataccc atgctcgcca gcgaaacggc atcattgccg ggtatggtcg
    11401 gtgggttggc cggtttttcg acaaacgccg tgatgttgcg ataggcatcc accgccatca
    11461 cgccaaaagc agatgcctct tcccgtggca cctcgataca gccgaccgta caacccgcac
    11521 cgcgaacgac gtggtctttc agcatgatcg agtagtccat tttgtaaaca tggtcacctg
    11581 ccaaaatgac gacgtactct ggatggctgg actgaatgat gtcgatgttc tgaaagatgg
    11641 catcagccgt gccccgatac cagtgctctt caccggcacg ctgctgcgct ggcagcatat
    11701 ccaccatttc attgagttcg gcgcgcaaaa aattccagcc gcgttgcaag tggcgcatca
    11761 gggaatgcga cttgtactgg gtcaccaccg ccatgcgacg aatgcccgaa ttcagacagt
    11821 tggacaatgc aaagtcaaca atccggaact tgccaccaaa atagaccgca ggctttgccc
    11881 ggctgtctgt caattgcttg agtcggctgc cacggccacc cgccagcacc agagccacgg
    11941 tatgtcggac caattgatgg gttgtaggaa ttttgtcaac agcaggcatg gttatctccg
    12001 gatctcgttt ttttatgcaa tgtgaggaaa tcgactgacc gcgcgcgttg cgcgctgctt
    12061 gggctcaggt tgccacgctg gggccactca tgccggtatg tcgggcgata ccggcaacct
    12121 gatcggccaa cgccgccaga tcttgcagcg cctgctgcac cggtacatcc tgcaaggccg
    12181 ggttggcctc gtaccgctcc agatacaggc gaagtgttgc gccttccgtg ccggtgcccg
    12241 acaggcgaaa aatcacccgt gcaccgtcat cgaaaatcag cctgatgccc tgtgcattcg
    12301 agaccgagcc atcaaccgga tcggcatagg aaaaatcgtc agccagggtc acggtgcgtg
    12361 tgccaaatga ctgccccggc aaacccggca gcgcagcgcg caaacccgtc atcagtgcat
    12421 tggcggcatc caccgcaaca ccctcgtagt cgtgccgggt atagtagttg cggccatacg
    12481 cggcccagtg ctcacgcacc agcgactcaa ctgagcgacc ggtagcaccc tgcaggctca
    12541 tccagaaaag aatcgcccac aggccatctt tttcgcgcac atggctggaa ccggtgccgt
    12601 agctttcttc gccgcacaga gtgacgcgac cggcatcgag cagattgcca aaaaacttcc
    12661 agccggtggg ggtctcaaaa cacgcaatgc ccagcttctt ggcgacccgg tcaaccgctg
    12721 ccgaggtagg catggagcgc gctacaccca gcaagccgtc tttgtaaccc ggcaccagac
    12781 gggcatgcgc tgtcatcagc gccaggctgt ctgaaggggt taccacaaag ttgcgcccca
    12841 ccaccatgtt gcggtcggca tcaccgtcca tcgcagcgcc aaagtcaggc gcgtcagact
    12901 gcgccatgtg ggcgatcaag tcctcggcat tgaccggatt ggggtcaggg tgcaggccac
    12961 cgaagtcttc gagcggttcg gcgttgacca cggtaccggc tggcgcaccc agttcacctt
    13021 cgataatggc tttggcgtag ggtccgccaa cggcgcacat ggcatctacc cgcatcgtga
    13081 aaccacgcgc aaacatgccg cgcagcatgc caaagtcaaa cagttcccgc atcactgcgg
    13141 catagtccgc caccgagtca atcacctcaa cttgcatctg ttcgaccgat tgctcgccaa
    13201 gctcgtcgat ggcaatgtct ggcgcatcac tggcgcgcac ctcgctgatc gtcagggtac
    13261 gggcataaac ggcttcagtc agggtctcag tggccgggcc accattgctg gcgttgtatt
    13321 tgatgccgaa gtcgccatcc gggccacccg ggttatggct ggctgacagc acaatgccgc
    13381 caaacgcctt gcgcctgcga atcacggcgc tgacagccgg tgtggacaaa atgccgcctc
    13441 ggcccagcaa tacccgctgc acgccgttgg cagcggccaa tttcaaaatg gtctgcaccg
    13501 ctacccggtt gtggtagcgc ccatctccgc cgagcaccag cgtctgtccg cgcaggcctt
    13561 gaccgtccgc agcggaagca ttgagaacat caaacagcga ctgaacgaaa ttctccaggt
    13621 acattggctg catgaacacg gtaacttttt tgcgcaagcc tgaggtgcct ggacgctggc
    13681 cggccaatgg ggttgtgggg tgggtttgaa tggtcatgat gaaaggtacc ttatgcaagt
    13741 gtgacgggtc tgaattttgc atcaatcgcc cgtccaaaac cggcgctgca tgccgattaa
    13801 aatttgcaat gttgatgtac ctcaatataa cgcatgtgct cgacaggcgt gccactgcca
    13861 ccggcaagac ggcgattttt taccccgcaa ccaaagaacc ctgtgaccta cgcctttgaa
    13921 acccgccgac gccgtacctt tgccatcatt tcccacccgg atgcgggcaa gaccacgttg
    13981 accgaaaaat tgctgctgtt ctccggggcg atccagatcg ccgggtccgt gaaggcgcgc
    14041 aaagccaccc gccacgccac cagcgactgg atggaaatcg aaaagcagcg tggcatctcg
    14101 gtggcatcaa gcgtgatgca gatggtttat cgcgaccatg tcatcaacct gcttgacacc
    14161 cccggccaca aggatttcag cgaagacacg taccgcgtgc tgaccgccgt tgattcggcg
    14221 ctgatggtga tcgatgccgc caacggggtt gagtcacaaa cccgccgcct gattgaagtc
    14281 tgccgccagc gcgacacacc gatcatcacc ttcatcaaca agatggaccg agaggtgcgc
    14341 gacccgcttg acatccttga tgaagtcgaa cgcgaactcg gcatgccctg cgtgccgatg
    14401 acctggccgg ttggtcaagg caaaaccttt ggcggcatca tcaatctgcg cacccgtggc
    14461 atgacggtgt tcgaggccgg cagcgagcgc cgcccgcagg acttcgagac catgccgctg
    14521 actgacaccg aagccctgac aaagcgcttt ggcttcgaga ttgacaccgc cttcgagagc
    14581 atggaactgg ccaccggtgc ctcgccaagc tttgaccgcg aggctttttt ggctggccag
    14641 caaaccccgg tattttttgg ctctggcgtg aacaactttg gcgtgatgga ggtgcttgac
    14701 gcgctgatcg atctggcccc ttcgccgggt ccgcgcacca gttcttttgt cgtcaacaag
    14761 cagccggtca tccggcaggt gcagcctgaa gatgagcctt ttagtggcgt ggtgttcaag
    14821 gtgcaggcca atatggatgc caaccaccgc gaccgcatcg cctttgtgcg catggcctcg
    14881 ggcaaataca cgcccgggct caagctcaag gtgatgcgca ccggcaaaga actgcgtccc
    14941 accagcgtcg tgaccttcat gagccagcgc cgcgaggcgg tggaagaagc ctacgccggt
    15001 gacattgtcg gctttaccac ccacggcggt gtgcagttgg gcgacaccat caccgacggc
    15061 tcggtgaacc tgctgtttac cggtctgccg ttttttgccc ccgagatgtt catgaccgtg
    15121 gtgctgaaga acccgttgcg caccaaacaa ttgcagcaag gcctggcgca attgggtgaa
    15181 gaaggcgcga ttcaggtgtt tcggcccgag ctgggtggca acatgctgct cggggccgtc
    15241 ggccaactgc agtttgaagt ggtgcagcat cgcctcaaag gcgaatacga tgccgacatc
    15301 cggctcgaag gcagccaata caccggtgcc cgctggatca ccgccgactc agccactgaa
    15361 ctgaaagact ttgtccacgc ctacccgcag cgcatggcgc gcgatgccgc cgacaccctg
    15421 gcctacctgt gcaccagccc gtatgacgtg cggctggcac aagagcggtt tcccaaaatt
    15481 cactttcacc cactgcgcga acacgccggg ctggcgctgc aaagtgccaa ttgagcgggc
    15541 aaaccagatg ctggcgctgt cgcaatggcc gctgcaggca cgtcgccaga tcatcggtgt
    15601 cttcaccgac attgacgaca cgctgaccac ccaccaggcc atcacacccg aagccttgca
    15661 ggcgctggtt gacctgaagg ccgccggact ggcggtgatt ccgatcaccg gcagaccagt
    15721 cggctggtgt gcgccgcacg cgcagggcga cgctgtgcag ggcgtgctgc cctggtcggt
    15781 cgatgccatg gtggccgaaa acggtggcgt cgcctttgtc ccggaaaata atggtcaaaa
    15841 tagcctccag cccttatgta gcaagcgcta caagctatca aaaatatact actctgatgc
    15901 tgctgcccgt gctgccagcc aactgagaat gcagcaagtg gcgcagcgca tcttgcaaca
    15961 agtgcccggt gccacactgt cgcgcgactc ggctgggcgc gagaccgacc ttgccatc
//
LOCUS       DAOSLL010000020        15865 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_3867_length_15865_cov_4.806135, whole genome shotgun sequence.
ACCESSION   DAOSLL010000020 DAOSLL010000000
VERSION     DAOSLL010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 15865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15865
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3867_length_15865_cov_4.806135"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..2115
                     /locus_tag="PLB25_01545"
     CDS             61..2115
                     /locus_tag="PLB25_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE40310.1"
                     /translation="MASKYKSEFLANMSHELRTPLNSLLLLAQSLSQNNSGNLTTDQV
                     ESARIIHSSGTDLLNLINEILDLSKIEAGRMELQFGSVPVADMADAARSSFAHMALVK
                     GLSLDVLVSPDAPADLNTDYKRVEQIIRNLMSNAIKFTESGGVTLRFGRPDAAANLSR
                     SALSPANALAISVKDTGIGIARDKHKPIFEAFQQADGSTARRFGGTGLGLTISRELAN
                     LLGGEIQLASEPGQGSTFTLHLPLAGVVQAAAPAEQARLPNPASPQRPRALASPGPSE
                     ASPRRQAYIPDDRDALAAGDQLIVVIEDDPKFAAIVLGKCHEKGFKCLAAADGETGLA
                     LIDKHLPSAVILDIRLPGMDGMMILNALKEDIRTRHIPVHMISVEDNLAESLRRGAVG
                     HAVKPLDQERLEGVFDRLKAAAAVKPRRLLVVEDDATARRATLDLLADEGVAVDAADS
                     GEEAIRLLRASHYDCVVLDLKLPDMDGQTVLERLEQEGISLPPVVVHTARDLTAREEL
                     NLREHAESIIVKGVRSPERLLDEVTLFLHRVVSQMPEKKRQIIANLHDADAALRDKQV
                     LIVDDDMRTMFAVSRLLTGRGMLALKAENGERALQLLQENPAIDLVLMDIMMPVMDGY
                     QAMQRIRAQERWRKLPIIALTAKAMPEDRQKCIAAGASDYLPKPIDPDRLFSMMRVWL
                     YG"
     gene            2108..2965
                     /locus_tag="PLB25_01550"
     CDS             2108..2965
                     /locus_tag="PLB25_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOE40311.1"
                     /translation="MADTPLSLDQPRADLETIEVELLLEAIWQRYGYDFRAYARATIE
                     RRLRQFVSNSGLASVSELIPKVLHDARFFSQLARYFSIPVTELFRDPFVYRAVREKVL
                     PLLCTWPHIKVWHAGCASGEEAYSMAIVLKETGLNQRATLYATDFNDEALERARAGIY
                     DIEKIQVATRNYQQAGGSGTFSDYYHAAYNAAAMEAPLRERIVFSNHNLMSDGIFGDM
                     QMVFCRNVLIYFNRELQERVLTLLTDSLVHGGFLCLGTKEDLQFFSHCERYEVIDRKA
                     QLYRKRSTP"
     gene            2962..3555
                     /locus_tag="PLB25_01555"
     CDS             2962..3555
                     /locus_tag="PLB25_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOE40312.1"
                     /translation="MSGAFVVIGGSAGGIEALELIFRQLPSDFAAPILIVEHLHPSDD
                     GALARHLAHLTGLRVLEPSDKTPIAAGHVYTAPANYHMLVERPGNISLSVDDRVNWSR
                     PSIDVLFESAAHAWGSALVAVILSGANGDGANGLKAVKVAGGRTIAQKPQDAASPQMP
                     QAAIDTGAVDEVLRADSIAQRLVMLCQCHDLVTRKKP"
     gene            3552..6242
                     /locus_tag="PLB25_01560"
     CDS             3552..6242
                     /locus_tag="PLB25_01560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE40313.1"
                     /translation="MSAVYEHAGFKQKILIVDDRPENLVALRAVLQVLALDFIEAASG
                     NEALAATLHHDFALAILDIQMPGMDGIELASLMRGEPRTRNLPIIFLTAAFGDAVNIF
                     KGYEAGAVDYIVKPYDARVLVSKVRVFLELHAANVALAERNDLLTGSEERYRALVMTV
                     PDIVYRIDGDGIFTFLNMAVKNLGYTPADLVGQHFSVLMLPQEVASVSRETVLGRAIG
                     VSGGAQQAPGLFDERRTGPRRTSGLEVHLLPHRSSHAMTNQAESAAANLITAEVNCSG
                     LYGVPRGDKRSVFLGTVGVIRDISERKMAETELRLHREHLQVLVGEQTAELQAQAVEL
                     EERNLQLHEHNASLEKRIAERTLDLKQALDEQLAAKAALEASTRTLRDTQFAMNYVGI
                     GIHWVDVETGRVLYSNRFAAQMLGYTEDEMQHLSVSDFDSNLAAGDFFQLSEFIRKKG
                     FTKFETALRTKDNCLVPVEVTVYYLAEPQGGNPRFISFITDISERLRIQAALLQAKEA
                     AEAASTAKSSFLANMSHEIRTPLNGIIGMAHVMRRNRPEPVQMSQIDKILASGKHLLG
                     IINNILDLSKIEIGGLMLEKRDFALADMVHVTLDAISDTVTDKGLSLHVRIADLPPAI
                     CGDETRLSQVLLNYLGNAAKFTAHGSITLSGQVLEETDTGYLLRFEVADTGVGLTREQ
                     QGRVFSAFEQADTSTTRHYGGTGLGLAINRRLAQLMGGEVGVTSTPGRGSAFWITARF
                     DKVHGEVNSVGLAPSINPGEALLAGHQGTRLLLAEDDSFNQDVARFLLESVGIELGVA
                     SNGVEAVNMVEHGDYALVLMDVQMPEMDGVEATKCIRRLSGRATMPIIAMTANAFDED
                     RQLCLAAGMNDFVSKPVDPDTLYATVLKWLRQVAQGACPR"
     gene            6319..7365
                     /locus_tag="PLB25_01565"
     CDS             6319..7365
                     /locus_tag="PLB25_01565"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOE40314.1"
                     /translation="MIELGYLAGLDNPQWRDGAVAAVLDSLYLRYPDLAQQFGPGGKN
                     AFRQDLHYHLDYLAGARLANDPGMFEKYALWLKTVLDSRGVPSQHLVESLELLAAHCT
                     DQLPAAQAEPLCAILRAGQQALKGNGLPMPYVELRLPALLRAAPYQQALLSGNHRAAG
                     ELMQAAMRSGCSLPEASVRLVQPAMYDIGRLWQENRITVAKEHLATAITQNVLAHAYL
                     EAKFAPPKERSAMFAGVAGNHHSLGLRMMSDAFETVGWQTVYLGGDVPTGALLREVDA
                     SRPDLLCLSLSMPIHLATAREAVAALRAEMGSRCPTIWVGGQATLLGKRVWQALDADG
                     WAYDALQALEQLAA"
     gene            7362..9221
                     /locus_tag="PLB25_01570"
     CDS             7362..9221
                     /locus_tag="PLB25_01570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE40315.1"
                     /translation="MNSSIESLNLRSRQMLDCMQHLTATVIAFVHEDGTLLESNEGLR
                     RLLARRSDPKSGNVAGFFIQPDFAQLVSAVAAADGRVHQGLITVGETTAAYVSLCGTV
                     HRQGHTLILVAEFDVAEMESLNASVVQLNDELTGVQRELARQRRHLEDMVEARTATLV
                     LAKEAAEVANQAKSTFLATMSHELRTPLNAIIGMTELALRRATDSVQRDQLLRVRQSS
                     SRLIATINDILDISNMEAGRLNLQAINFDLDGVLDKLRTLLGEQASHKGLTLTLATAP
                     GIGRQPLAGDPLRLGQVLSSLLDNAIKFTEQGSVSLHVGLESDDAAGVMLRAEVRDTG
                     IGIAARDQQRIFAVFQQADGSSTRKFKGVGLGLAICKRLVKMMGGEIGVVSQPGGGST
                     FWFTARLHRQPVPAAGPGPTVDSVPAALLTIPGLEVARGLKVLKGQVALYLRYLRKFS
                     VDHAQDMTRLRGHLAASDAEGARRTAHNLKGTAGNMGATGVQQLATELDAAFKSGAAS
                     TEVERLIAALDSELGQLAAALELALPAESVTVSLAQVDWALLRQVLTELEPLLLSGNL
                     KANRLVSTHAALLKAALGPLAAQFEWQLAQFLHAEALKTLAQARAQLDEKWTE"
     gene            9236..10396
                     /locus_tag="PLB25_01575"
     CDS             9236..10396
                     /locus_tag="PLB25_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE40316.1"
                     /translation="MAATPATILLVDDDSGNLGTLAELLSPSYDVLVALAGQRALQIA
                     ASNPRPDLILLDVMMPEMDGYAVLARLRENQATADIPVIFVSGLDSVEDEEKGLSLGG
                     VDYITKPYHPPIILARVHTHLELKRARDLLNDHAHALESEVARRTAELRVAKDAAECA
                     NRTKSEFLANMSHELHTPMNGILGMIEITLGAAGVPDKLRNYLQVAHDSAKGLNAVLS
                     DILTYVSLIDGRTERLQRPFSPARLIDDVTACFASAARDKNLSLARSCAPDLPTEVCG
                     DAPKLQLVLTRLLDNAIKFTQVGSVSVSVKASPGLLTFQVEDTGCGVAADRREAIFEA
                     FTQADGSRTRRHGGAGLGLSIAKAMVGLMGGQIWVESQPGQGSCFFLLVPVG"
     gene            10556..10906
                     /locus_tag="PLB25_01580"
     CDS             10556..10906
                     /locus_tag="PLB25_01580"
                     /inference="COORDINATES: protein motif:HMM:NF025197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HOE40317.1"
                     /translation="MKKLSSILVGLLLALPMCAFAAGAIAVDDEEGETEPGYGFVTGA
                     SSRDAAAKGALKQCKDSGNDNCKVVARFDACGAYAASKKYYGAGWGASKQKAESMALE
                     KCSGNCKIIVSECE"
     gene            complement(11036..11539)
                     /locus_tag="PLB25_01585"
     CDS             complement(11036..11539)
                     /locus_tag="PLB25_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE40318.1"
                     /translation="MKNTVAITTGLIVAHLAGAVWAQQDGSMADSPAPKLRLQVSPYT
                     LHFSEDTRHENVYMAGLEREHANGKLDGFTLFSNSFGQESVYLYPWGGVYNSIWGINA
                     LSFKWTAGLLYGYKKPYENKVPLNYRGFSPGAIIALAYEFKPGWSAQLDLLGTAGLML
                     QLNVPLQ"
     gene            11606..12442
                     /locus_tag="PLB25_01590"
     CDS             11606..12442
                     /locus_tag="PLB25_01590"
                     /inference="COORDINATES: protein motif:HMM:NF015892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE40319.1"
                     /translation="MLWALLGTSGCAWLDTWSRQKIYRPTPTDSMVVVDLPAGEEHFF
                     LPVPQTGEPQRISLWWLPHATKNAPTLLYLHGTFRNLPQNLPKINALREAGFAVLAVD
                     YRGWGQSSVIVPSEQSILADARVAWAELVRREPRAGQRVIYGHSMGSGVAVTLASELP
                     TQADYGALILESAFTGFAGVARSAGFLASLLSGFSNERFASIDKIAQVHAPLLMIHGS
                     HDHTVPMHLGQQLFEAARGPKQWLTIEGGQHSDLHQVGQAPYQAALAAFKAHYLSQPE
                     QR"
     gene            complement(12422..13045)
                     /locus_tag="PLB25_01595"
     CDS             complement(12422..13045)
                     /locus_tag="PLB25_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOE40320.1"
                     /translation="MNLVFDFGAVLFTWQPGQFLLQIFPGQVGSLEAARHLAHQVVGH
                     PDWHDFDRGVLDTAALIERTSQRLDLPLASVQALINGIGERLIPIDGTLALLQELRQR
                     RLTQDGVTGLYYLSNMPDVYARVLEANFAFLQWFDGGIFSCDVKHIKPDPAIYQLLQD
                     RYRLEPTTTLFIDDLKANVAVARTLGWQGIHYESPQQLQSQLALLGL"
     gene            complement(13071..14639)
                     /gene="pgi"
                     /locus_tag="PLB25_01600"
     CDS             complement(13071..14639)
                     /gene="pgi"
                     /locus_tag="PLB25_01600"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HOE40321.1"
                     /translation="MKTTRCDRTPVWSVLQQQFEQTGRTFDLRTAFAADPKRFESFSL
                     TAPHVFADLSKNLIDAATQASLLELAQQSGLEQHRDAMFAGQTINNTEQRAVMHWLLR
                     NPAVAQMDKARPAIKSLANELQDVHTTLDAMLAYATAVRADDSITDVVNIGIGGSDLG
                     PQMAVVALNEFVTPAKRFHFVSNVDGHELAAVLKKIKPESTLFLIASKTFTTIETMTN
                     ARSAKAWFEASGGTDVARHFAALSTNVAAANAFGISTTFGFWDWVGGRYSVWSAIGLP
                     LAIAIGAEGFRQFLAGAHAMDEHFRVAPLAQNLPVRLGLLDVWYRNFHGFTSRSVAPY
                     HCALKRYPAYLQQLEMESNGKRVDASGAALPFGTSPVLWGEPGTNGQHAYFQMLHQGT
                     DVVPVEFVAVKKPRASLPGHHTLLLANVLAQAQALMQGKADAGGHKHFPGNRPSSFLL
                     LDELTPASLGALIALQEHRVFVSGSLWGINCFDQWGVELGKVLAKEVEARLGSGDVSG
                     LDGSTAGLLEKLRA"
     gene            complement(14636..15589)
                     /gene="tal"
                     /locus_tag="PLB25_01605"
     CDS             complement(14636..15589)
                     /gene="tal"
                     /locus_tag="PLB25_01605"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HOE40322.1"
                     /translation="MNQLDALRQFTTVVADTGDFRQMAAFQPQDATTNPSLILKAVQK
                     PEYRSLLDETVARHGDRALDEVMDRLLVRFGCEILAIIPGRVSTEVDARLSFDANATL
                     TRAERLIELYQAQGIHIDRVLIKVAATWEGIEAAAELERRGIHTNLTLLFSFCQAVAC
                     AQARVQLISPFVGRIYDWYKKSAGTTWVEADNALAQDPGVKSVRQIFNYYKKYGISTE
                     VMGASFRNVGQIIALAGCDLLTISPELLALLAQSDAPLVPALDARQAQTLDIAHVTLD
                     QAAFRWALNQDAMATEKLAEGIRAFSADAIKLDQLLKGSYP"
     gene            complement(15600..15734)
                     /locus_tag="PLB25_01610"
     CDS             complement(15600..15734)
                     /locus_tag="PLB25_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40323.1"
                     /translation="MVVSPDLPDVLCLGAAVGQTLADHAVWLAAVVPVVRFTRAASIA
                     "
BASE COUNT         3203 a         4767 c         4814 g         3081 t
ORIGIN      
        1 tgctagtcca tcgctgcttg tgatgtcaag ttccacacaa aacggcatag agcccttgca
       61 ttggccagca aatacaagtc cgaatttctg gccaacatgt cgcacgagtt gcgcacgcca
      121 ctcaacagcc tgctgctgct ggcgcaaagc ctgtcacaaa acaatagcgg caacctgacc
      181 accgaccagg ttgagtcggc ccgcatcatc cacagcagtg gcaccgacct gctcaatctg
      241 atcaacgaaa ttctggacct gtcgaaaatc gaggccgggc gcatggaatt gcagttcggc
      301 agcgtgccgg ttgccgacat ggccgatgcc gcgcgctctt ctttcgccca catggccctg
      361 gtcaagggcc tcagcctgga tgtgctggtc agccccgatg ccccggctga cctgaacacc
      421 gactacaagc gggttgagca gatcatccgc aacctgatgt ccaacgccat caagttcacc
      481 gagtcgggcg gtgtcacgct gcgcttcggg cggcccgatg ccgccgccaa tctgtccaga
      541 agcgccctga gcccagccaa tgccctggca atctccgtca aagacaccgg cattggcatt
      601 gcgcgggaca agcacaagcc gattttcgag gcctttcagc aggccgatgg cagcacggca
      661 cggcgttttg gcggcaccgg gcttgggctg accatctcgc gcgaactggc caacctgctc
      721 ggtggcgaaa tccagctcgc cagcgaaccc ggccagggtt ccaccttcac gctgcacctg
      781 ccccttgccg gggtggtgca ggctgcggcc cccgctgagc aggcccgact gccaaacccg
      841 gccagcccgc agcgcccgcg cgcgctcgcc agccctgggc cgtcagaggc aagcccaagg
      901 cgacaggcct acatcccgga cgaccgcgac gcccttgctg cgggcgacca actgattgtg
      961 gtgatcgagg acgaccccaa gttcgccgcc attgtgcttg gcaagtgcca cgaaaagggt
     1021 ttcaagtgcc tggccgctgc cgatggcgaa accggcctgg cgctgatcga caaacatctg
     1081 ccgtcggcgg tgattctgga catccgccta ccgggcatgg acggcatgat gatcctgaat
     1141 gccctcaagg aagacattcg cacccgccac atcccggtgc acatgatttc ggtggaagac
     1201 aacttggctg agtcgctgcg ccgaggtgcc gtcggccacg ctgtcaagcc gcttgaccag
     1261 gagcgtctgg aaggcgtgtt cgaccggctc aaagcagcgg cggcggtcaa gccccggcgc
     1321 ctgctggtgg tcgaggacga cgcaacggcg cggcgcgcca ctcttgacct gctggccgat
     1381 gagggtgttg cggtcgatgc agccgacagt ggcgaagagg cgatccgcct gctgcgcgcc
     1441 agccactacg actgcgtggt gctggacctg aaactgcccg acatggacgg ccagaccgtg
     1501 ctggagcgcc tcgaacagga aggcatcagt ttgccgccgg tggtggtgca caccgcgcgt
     1561 gacctgactg cccgcgagga actcaacctg cgcgaacacg ccgagtcgat catcgtcaag
     1621 ggcgtgcgct cgccggagcg cctgctcgac gaagtcacgc tgtttcttca ccgggtagtc
     1681 agccagatgc ccgagaaaaa gcgccagatc atcgccaacc tgcatgacgc agatgccgca
     1741 ctgcgcgaca aacaggtgct gatcgtggac gatgacatgc gcaccatgtt tgccgtgtcg
     1801 cgcttgctca ccgggcgcgg catgctggcc ctgaaggccg agaatggcga acgtgcactg
     1861 cagttgttgc aggaaaaccc ggccattgac ctggtgctga tggacatcat gatgccggtg
     1921 atggatggct accaggcgat gcagcgcatc cgcgcccagg agcgctggcg caagctgcca
     1981 atcattgccc tgaccgccaa ggccatgccc gaagaccggc agaaatgcat cgcggcgggc
     2041 gccagcgact atctgcccaa gccgattgac ccggaccgtt tgttctcgat gatgcgggtg
     2101 tggttatatg gctgatacac ccttgtccct ggaccagccc agagccgacc ttgaaaccat
     2161 cgaggtcgag ctgctgctcg aagccatctg gcagcgctat ggttacgatt tccgcgcgta
     2221 cgcgcgcgcc accatcgagc gccgactgcg ccagtttgtg tccaacagcg gtctggcctc
     2281 ggtcagcgag ctgattccga aggtgctgca cgacgcacgg ttcttctcgc aactggcgcg
     2341 ctacttctcg attccggtca ccgaactgtt ccgtgacccc tttgtgtacc gcgcggtgcg
     2401 tgagaaagtg ctgccgctgc tgtgcacctg gccgcacatc aaggtctggc acgccggttg
     2461 tgccagcggc gaggaagcct attcaatggc tatcgtgctc aaggaaaccg gcctgaacca
     2521 gcgcgccacc ctgtatgcca ccgatttcaa tgacgaggca cttgagcgtg cccgcgccgg
     2581 catttatgac atcgaaaaaa ttcaggtggc cacgcgcaac taccagcagg ccggcggcag
     2641 tggcaccttc tcagactact accacgcggc ctacaacgct gctgccatgg aggccccgct
     2701 gcgagagcgc atcgtctttt caaaccacaa cctgatgtcg gacggcatct ttggcgacat
     2761 gcaaatggtg ttttgccgca atgtgctgat ctatttcaat cgcgaattgc aagagcgggt
     2821 gctgacgctg ttgaccgaca gcctggtaca cggtggcttc ctgtgcctgg gcaccaagga
     2881 agacctgcag tttttcagtc actgcgagcg ctatgaggtg atcgaccgca aggctcagct
     2941 ttaccgcaag cgcagcacac catgagcggc gcgtttgtcg tgatcggtgg ttcggctggc
     3001 gggattgagg cgctggagtt gatctttcgc cagttgccga gcgactttgc cgcaccgatc
     3061 ctgattgtgg agcacctgca tccaagcgac gacggcgctt tggcgcgcca tcttgcacac
     3121 ctgacgggtc tgcgggtgct tgaacccagc gacaagacac cgatcgcagc cggacacgtt
     3181 tacaccgcac ccgccaacta tcacatgctg gtcgaacgcc caggcaacat cagcctgtcg
     3241 gtcgatgacc gggtcaactg gtcgcgcccg tcaattgacg tgctgtttga gagtgccgcc
     3301 cacgcctggg ggtcggcgct ggtggcggtg atcctgtcgg gtgccaatgg cgacggtgcc
     3361 aatgggttga aagccgtcaa ggtggcgggc gggcgtacca ttgcccaaaa gccgcaggac
     3421 gcagccagcc cgcaaatgcc gcaagcggcg attgacaccg gggccgttga tgaggtactg
     3481 cgagccgaca gcatcgcgca gcgactggtc atgctgtgcc aatgccatga cttggtcaca
     3541 aggaaaaagc catgagtgcc gtttatgaac atgccgggtt caagcagaag attctgattg
     3601 ttgacgacag gcccgagaac ctggttgcgc tgcgcgcggt gctgcaagtc ctggcacttg
     3661 atttcatcga agccgccagc ggcaacgagg cgctggcagc cacgctgcac catgactttg
     3721 cgctggccat tctggacatc cagatgcccg gcatggatgg cattgagttg gccagcctga
     3781 tgcgtggcga gcccagaacc cgcaatctgc cgatcatctt cttgacggcg gcctttggcg
     3841 acgcggtcaa cattttcaaa ggctacgaag ccggtgcggt ggattacatc gtcaaaccct
     3901 atgatgcgcg ggtgctggtt tccaaggtcc gggtttttct ggaactgcat gctgccaatg
     3961 tggcgctggc cgagcgcaac gacctgctga cgggctcaga ggagcgatac cgcgcgctgg
     4021 tgatgacggt gcccgacatc gtctatcgca tcgacggcga tggcatcttt accttcctca
     4081 acatggcggt gaaaaatctt ggctacacgc cagcagatct ggtcggccag cacttctcgg
     4141 tgctgatgct gccgcaggaa gtggcttcgg tcagccgcga aaccgtgctc ggccgcgcca
     4201 tcggagtatc aggcggggcg cagcaggcac caggcctgtt tgacgagcgg cgtaccgggc
     4261 cacgccgcac ctctggcctt gaggtgcatc tgctgccgca tcgcagcagc cacgccatga
     4321 cgaaccaggc cgaatctgcg gctgcgaacc tcatcaccgc tgaggtcaat tgttccggcc
     4381 tctacggtgt gccgcgtggc gacaagcgct cggtgtttct aggtaccgtc ggggtcattc
     4441 gtgacatctc cgagcgcaag atggccgaga cagaactcag gctgcaccgt gaacatctcc
     4501 aggtgctggt tggcgagcag accgccgaac ttcaggcaca ggctgttgag ctcgaagaac
     4561 gcaacctgca gttgcatgag cacaatgcaa gcctggagaa gcgcatcgct gaacggacct
     4621 tggacttgaa gcaagcgctc gacgagcagc ttgccgccaa ggcagcgctg gaggcctcca
     4681 cccgaacact gagagacacc cagtttgcca tgaactatgt cggtatcggc atccactggg
     4741 tcgatgtcga gactggccgc gtgctctatt cgaaccgctt tgcggcgcag atgctgggtt
     4801 acaccgaaga tgaaatgcag cacctcagcg tgtccgattt cgattcgaac ctggcggccg
     4861 gagacttctt tcagctctcc gaatttatcc gcaagaaagg gtttacaaag ttcgaaacgg
     4921 cgctacgcac caaggacaat tgcctggtgc cggttgaagt cactgtttat tacctggccg
     4981 aaccacaggg cggtaaccca cgcttcatca gtttcatcac tgacatcagc gaacggctgc
     5041 gaatccaggc ggcgctgttg caagccaaag aggctgccga agccgcaagc actgcaaaaa
     5101 gctcgtttct ggccaatatg agccacgaga ttcgcacccc cttgaatggc atcatcggaa
     5161 tggcgcatgt gatgcgccgc aaccgccccg agccggtgca aatgtcacaa attgacaaga
     5221 ttctggcctc tggcaagcac ctgctgggca tcatcaacaa catcctggat ttgtccaaaa
     5281 tcgagatcgg cggactgatg cttgaaaagc gggactttgc cctggcagac atggtgcacg
     5341 tcacgcttga tgccatcagc gacacggtga ccgacaaggg cctgagcctg cacgtcagaa
     5401 ttgccgacct gcccccggcg atttgcggcg atgaaacccg cttgtcacag gtactgctca
     5461 attacctggg caacgccgcc aaattcaccg cgcacggcag catcacgctg agcgggcagg
     5521 tgctggaaga aaccgatacc ggttacctgc tgcggtttga agtcgccgac actggcgtag
     5581 gcctgacccg ggaacagcag ggacgagttt tctcggcctt cgagcaggct gacacatcca
     5641 ccacgcggca ttacggcggc accgggctgg gtttggccat caatcgccgc ctggcccaat
     5701 tgatgggtgg cgaagtgggt gtcaccagca ccccaggccg gggcagtgcc ttctggatca
     5761 cggcacgctt tgacaaggtg catggggaag tcaattccgt gggccttgca ccgtcaatca
     5821 atcctggtga ggcattgctc gccgggcatc aaggcacgcg cttgcttttg gccgaagacg
     5881 atagcttcaa tcaggacgtg gcacgctttc tgctggagtc ggtcggcatt gaacttgggg
     5941 tggccagcaa cggcgttgag gcagtgaaca tggtggaaca cggcgactac gccctggttc
     6001 tgatggacgt tcagatgccc gagatggacg gtgttgaagc caccaagtgc atccgccgct
     6061 tgtccggtcg cgcaacgatg cctatcatcg ccatgacggc caatgccttt gatgaggacc
     6121 gccagctttg cctggccgcc ggcatgaacg actttgtcag caagccggtt gatcctgaca
     6181 ccctgtatgc gaccgtgctc aagtggttgc ggcaagtcgc tcagggcgcg tgcccgcgat
     6241 gatgttcacg accgccgggc gcaacgccac agttttgacc cgatacccgc aaaatgattc
     6301 gacgagaggt gctgccacat gattgaactt ggctatctcg ccggtttgga taacccgcaa
     6361 tggcgcgacg gtgccgtcgc cgccgtgctg gattcgcttt atttgcggta cccggacctg
     6421 gcccagcagt ttggccccgg tggaaaaaac gccttccgcc aggaccttca ttaccacctc
     6481 gattacctgg caggtgcccg cctggccaat gaccctggga tgtttgaaaa atatgcactc
     6541 tggctcaaga cggtgctgga cagccgtggc gtgccgagcc agcatctggt ggagtcgctt
     6601 gaactgcttg cggcgcactg tactgaccag ttgcccgcag cccaggccga gccgttgtgc
     6661 gccattctca gggctggcca acaggcgctc aaaggcaatg gtttgccaat gccttatgtt
     6721 gagcttcgcc tgccagcgct gctcagggcg gcgccgtacc agcaagcgct attgagcgga
     6781 aaccatcggg ccgctggtga actgatgcaa gctgccatgc gctctggctg cagcttgcca
     6841 gaggcttcgg tacgcctggt ccagccggcc atgtacgaca ttggtcgcct ttggcaagaa
     6901 aaccggatca ccgtcgccaa agaacatctg gccacggcga tcacgcaaaa tgtgctggcg
     6961 catgcctatt tggaagccaa atttgccccg cccaaggagc gcagcgccat gtttgccggt
     7021 gtggcgggca accatcacag cctgggtttg cgcatgatgt ccgatgcctt cgagacggtt
     7081 ggctggcaaa cggtttacct ggggggcgat gtgccgaccg gtgccttgct gcgcgaagtg
     7141 gatgcttccc gccctgacct gctgtgcctg tcgctgtcga tgccgattca tctggcgacg
     7201 gcgcgcgagg cggtggccgc attgcgggct gaaatgggca gtcgatgccc gaccatctgg
     7261 gtcggtgggc aggccacctt gctcggcaaa cgggtctggc aagcgctgga tgccgatggc
     7321 tgggcttacg atgcgctgca ggcactggaa caattggcag catgaactca tccatcgaat
     7381 cgctgaacct gagaagccgt cagatgctgg actgcatgca gcatctgacg gcgacggtca
     7441 ttgcctttgt gcatgaagac ggaacattgc tggaaagcaa cgagggcttg cggcgcctgc
     7501 tggcccgacg cagcgacccg aaatcgggca atgtcgcagg cttttttatc cagccggact
     7561 ttgcgcaact ggtctcagcc gttgccgcag cagacgggcg cgttcaccaa gggctgatca
     7621 cggtcggcga aaccacagcg gcttatgtat cactgtgcgg cacagtgcac cgacaaggcc
     7681 acacgctgat tctggtggcc gagttcgatg tggcagaaat ggagtcgctt aacgcgtcgg
     7741 tggttcagct caacgacgaa ttgacagggg tacaacgcga acttgcgcgc caacggcgcc
     7801 acttggaaga catggttgaa gcccgcacgg ccaccctggt gcttgccaaa gaagccgctg
     7861 aagtcgccaa ccaggcgaaa tccacttttc tggccaccat gagtcatgaa ctgcggacgc
     7921 cgctgaacgc catcatcggc atgaccgaac tggccttgcg ccgagccacc gacagcgtgc
     7981 aacgggatca gctcttgcgg gttcgacaga gttccagccg actgattgcc accatcaacg
     8041 acattctcga catttccaat atggaggcgg ggcggctgaa cctgcaggcg atcaactttg
     8101 acctcgacgg cgtgctggac aaactgcgca cgctgctggg tgaacaggca tcgcacaagg
     8161 ggctgacatt gacgctggcc accgcaccgg gcattggccg acagccgctt gcgggtgacc
     8221 cgcttcggct gggacaggta ttgagcagcc tgcttgacaa tgcgatcaag ttcaccgagc
     8281 agggcagcgt cagtctgcac gttgggttgg aaagcgacga tgccgctggc gtgatgctgc
     8341 gcgccgaggt ccgcgacacc ggcattggca tcgcggcacg ggatcagcag cgcatttttg
     8401 ccgtctttca acaggccgat ggctcatcca cccgaaagtt caagggtgtc ggcctcgggc
     8461 tggcaatctg caagcggctg gtcaaaatga tgggcggcga aatcggtgtg gtgagccagc
     8521 cgggcggcgg cagcacattc tggttcaccg cgcgcctgca ccgccagccg gtgcccgccg
     8581 cagggcccgg gccgaccgtc gacagcgtgc cggcagccct gctgaccatc cccggtctgg
     8641 aagttgcgcg cgggctcaag gtgctcaaag ggcaggtggc actttatctg cgctatttgc
     8701 gaaaattttc ggtcgatcac gctcaggata tgacccgact gcgcgggcat ctggcggcca
     8761 gcgatgctga gggagcccga cgcacggccc acaacttgaa aggcactgct ggcaatatgg
     8821 gggccaccgg ggtgcaacaa cttgccactg aactggatgc tgcattcaag tcaggtgcag
     8881 ctagcaccga agttgagcgg ctcatcgcag cgctcgacag cgagctgggg caactggcgg
     8941 cagcgcttga attggccctg cccgcagagt cagtaacagt gtccctggcc caggtcgatt
     9001 gggccctgct gcgtcaggtg ctgactgagc tcgagcccct gttgctttcg ggcaatctga
     9061 aggccaaccg cctggtcagt acccatgcag ccctgctcaa ggcagcactg ggaccgctgg
     9121 ccgcccagtt tgagtggcaa ctggcacagt tcctacacgc tgaggctttg aagacgctgg
     9181 cgcaggcgcg cgcacaattg gacgaaaaat ggaccgaatg aacaaggaga acgcgatggc
     9241 tgcgacaccg gcaacaatcc tgctggtcga tgacgactcc ggcaatctgg gcactctggc
     9301 tgagttgctg agtccaagct acgacgtgct ggtggctttg gcggggcaac gcgcattgca
     9361 gattgctgcc agcaacccgc ggcccgatct gatcctgctc gacgtgatga tgccggaaat
     9421 ggatggctac gccgtgctgg cccgcctgcg cgaaaaccag gccactgctg atattccggt
     9481 catatttgtc agcggcctgg attcggtcga agacgaagaa aagggcctca gcctcggtgg
     9541 cgtggactac atcaccaagc cttaccaccc gcccatcatt ctggcgcggg tgcataccca
     9601 cctggaactc aagcgtgcgc gcgacctgct caacgaccac gcgcacgcac tggaaagcga
     9661 ggttgcccgc cgcaccgccg agctgcgtgt tgccaaggat gctgcagagt gtgccaatcg
     9721 gaccaagagc gaatttctcg ccaacatgag ccacgaactg cataccccca tgaacgggat
     9781 tctcggcatg atcgaaatca cgctcggcgc tgccggtgtg ccggacaagc tcagaaacta
     9841 tttgcaggtg gcgcacgatt cggccaaggg cctgaatgcc gtgctgtcag atattttgac
     9901 ctatgtttcg ctcatcgacg gtcgcactga gcgactgcaa cggccttttt cgccggccag
     9961 gctgatcgac gatgtgaccg cctgttttgc cagtgccgcc cgcgacaaga atctgtcgct
    10021 ggcccgttcc tgcgccccgg acctgcccac tgaagtctgt ggtgacgcgc ccaagctgca
    10081 actggttctg acacggctgc tcgacaacgc catcaagttc acacaggtcg gctccgtcag
    10141 tgtcagtgtc aaggccagcc ccggcctgct gacttttcag gtcgaagaca ccggctgtgg
    10201 tgttgcagca gatcggcgcg aggcgatttt tgaggccttc acccaggccg atggctccag
    10261 aacccgtcgg catggtggtg ccgggctggg cctgtcgatc gccaaagcga tggtcggctt
    10321 gatgggtggc cagatttggg tcgagagtca gccgggccag ggaagctgct tttttctgtt
    10381 ggtaccggtc gggtgagtgc ggcacatccc ggctgatgcc gacgcgaatt cggccccaca
    10441 tctggcagcg caagacgcag ccttggtttt tcgtcacaat gccaaccgga accggctgcc
    10501 tttaaccggt acccgctcca tgcctctcgc cccccatcat tccaaggatt tgaatatgaa
    10561 gaaattgtca tcaattttgg tcggtctgct attggccttg cccatgtgcg catttgcggc
    10621 aggtgccata gcggtcgatg atgaagaggg tgaaaccgaa cccggctatg ggttcgtgac
    10681 cggcgcaagt tccagggatg cggcggccaa aggcgccctg aagcagtgca aagacagtgg
    10741 caacgacaac tgcaaggtgg ttgcccgatt cgatgcctgc ggtgcttatg ccgcttcaaa
    10801 aaaatactac ggtgctggtt ggggtgccag caagcagaaa gcagagagca tggcgctgga
    10861 aaaatgttca ggcaattgca aaatcatcgt ctctgagtgc gaatagttgc cctggcaaac
    10921 cgcccgccag ggcggttgcc gaccactcgg agcctgtttt ttgtttcatt attgatagct
    10981 gtcagcgcat atacagcaag ggctacaagg ctttttaaat ctcaatacta tggttttatt
    11041 gcagcggcac attgagctgc aacatcaagc ccgccgtccc cagcaggtcg agttgcgctg
    11101 accagccggg cttgaattca taagccaggg caatgatggc accgggtgaa aagcccctgt
    11161 aattcagcgg caccttgttc tcatagggct ttttgtagcc ataaagtaaa ccagcggtcc
    11221 acttgaatga caaggcattg atgccccaaa tgctgttgta aacaccgccc caagggtaga
    11281 ggtaaacact ttcctggcca aatgagttgg aaaacagcgt gaacccgtcc agcttgccgt
    11341 tggcatgctc gcgctccagc cccgccatgt agacgttctc atgtcgggta tcttcgctga
    11401 aatgcagggt ataggggctg acctgcaacc tcagtttggg tgccgggctg tcggccatgc
    11461 tgccgtcttg ctgagcccag acggcaccgg caagatgggc cacaatcagt cctgtggtga
    11521 tcgctactgt atttttcatg ggcaagtctt gcgttttgtc atgtttggga tgcgcagtgt
    11581 aacggctgat cttttcaaaa ttctgatgct ctgggcgctg ttgggcacca gtggatgtgc
    11641 ctggctggac acctggtctc gccagaagat ttaccgcccg accccgacgg acagcatggt
    11701 ggtggtggat ttgccggccg gggaggaaca tttttttctg cccgtgccgc agaccggcga
    11761 gccccagcgt atctcgctgt ggtggctgcc gcacgccacc aaaaatgcac caacgctgct
    11821 ttacctgcac ggcacgtttc gcaatttgcc gcaaaacctg cccaagatca acgcgctgcg
    11881 cgaggccggt tttgccgtgc tggcggtcga ttatcggggc tggggtcaga gttcagtgat
    11941 cgtgccctcg gagcaaagca tcctggccga tgcccgcgtt gcctgggccg agctggtgcg
    12001 ccgcgaaccg cgcgcaggcc aacgggtaat ctacggccac tcgatgggca gtggtgttgc
    12061 agtgactctt gccagcgagt tgccaaccca ggccgactat ggggcgctga ttctggaatc
    12121 ggccttcacc ggttttgccg gtgtggcacg ctcggccggt tttctggcca gcctgctgtc
    12181 aggttttagc aacgagcgct ttgcctcgat tgacaagatt gcccaggttc acgcgccttt
    12241 gctgatgatt catggcagcc acgaccacac tgtcccgatg cacttggggc agcagttgtt
    12301 cgaggcagcc agggggccca aacaatggct gaccatcgag ggcgggcagc acagcgacct
    12361 gcaccaggtc ggccaggcgc cctaccaggc ggcgctggcg gctttcaagg cgcattacct
    12421 gtcacagccc gagcagcgct aactgcgact gcagttgctg cggcgactca tagtggatgc
    12481 cctgccaacc caatgtgcgg gccaccgcca cattggcttt caggtcgtct atgaacagcg
    12541 ttgtggtcgg ctcaagccga tagcggtctt gcagcaactg gtaaattgcc gggtcgggct
    12601 tgatgtgttt gacatcacat gaaaagatgc cgccgtcaaa ccactgcaag aacgcaaagt
    12661 ttgcttccag cacccgtgca taaacgtctg gcatgttgga caggtaatac agcccggtga
    12721 caccgtcctg cgtcagacgg cgctggcgca gttcctgcag cagcgccagc gtgccgtcga
    12781 tcggtatcag gcgttcccca atgccgttga tcagggcctg gaccgatgcc agcggcagat
    12841 caagccgttg tgaggttcgc tcaatcaatg cagcggtgtc cagcacacct cggtcaaaat
    12901 catgccagtc gggatggcca accacctgat gtgccaaatg cctggctgcc tccagcgaac
    12961 cgacctgacc gggaaagatt tgcaacagga attggcccgg ctgccaggta aacagcacgg
    13021 caccaaagtc aaacaccaga ttcatcgaca agcggcggct gatggcccga tcaagcccgc
    13081 aatttttcca gcagaccggc ggtggagccg tccagcccgc tgacatcgcc gctgcccagt
    13141 cgcgcttcca cctctttggc cagcaccttg ccaagctcga caccccattg gtcaaaacag
    13201 ttgatgcccc acaggctgcc actgacaaac acgcggtgct cctgcaaggc aatcagcgca
    13261 cccagactgg ccggggtgag ttcatcgagc agcaaaaagc tgcttgggcg gttgccggga
    13321 aaatgcttgt ggccacctgc atcagccttg ccctgcatca gcgcctgagc ctgcgccagc
    13381 acattggcca gcagcagcgt gtggtggcca ggcaggctgg ctctgggctt tttgacggcg
    13441 acaaactcga ccggcaccac gtcggtgccc tggtgcagca tctggaagta ggcgtgctgg
    13501 ccgttggtgc caggttcgcc ccacaacacc ggcgaggtgc caaagggcag cgcagcgcca
    13561 ctggcatcca cgcgcttgcc gttgctttcc atttcaagtt gttgcaaata agccgggtag
    13621 cgcttgagcg cgcaatggta gggtgccacg ctgcggctgg taaagccgtg gaaattgcgg
    13681 taccagacat ccagcagccc caggcgaacc ggcaggtttt gcgccagcgg ggcgaccctg
    13741 aagtgttcgt ccatggcatg cgcgccagcc agaaactgcc ggaatccttc tgcaccaatg
    13801 gcaatggcca gcggcaggcc aatggccgac cagaccgaat aacgcccgcc gacccagtcc
    13861 cagaaaccaa aggtggtgct gatgccaaac gcgttggcgg ctgccacatt ggtgctcagc
    13921 gccgcaaaat gccgtgccac atcggtgccg ccgctggcct caaaccaggc cttggcagag
    13981 cgcgcattgg tcatcgtctc gatggtggta aaggtcttgc tggcaatcaa aaacagcgtg
    14041 ctctcgggct tgattttttt cagcacggca gcgagttcat gcccatcaac attcgagaca
    14101 aagtgaaagc gcttggccgg agtgacaaat tcgttgagtg ccactaccgc catctgtggc
    14161 cccaggtccg agccaccaat gccgatattg accacgtcgg tgatgctgtc gtcagcgcgc
    14221 acagcggtcg catacgccag catggcatcc agcgtggtgt gcacatcctg cagttcattt
    14281 gctagcgact tgatagctgg tcgcgcttta tccatctgtg ctacagccgg atttctcaat
    14341 aaccagtgca tcaccgcgcg ctgctcggtg ttgttgatgg tttggccggc aaacatcgca
    14401 tcgcggtgtt gctccaggcc gctttgttgt gccagctcaa gcaggctggc ctgggtggcg
    14461 gcatcgatca agttcttcga cagatcggca aatacgtgtg gcgcagtcag gctgaaggac
    14521 tcaaaacgct ttgggtcggc ggcgaaggcg gtgcgcaggt caaacgtgcg accggtttgc
    14581 tcaaattgtt gttgcaaaac gctccagact ggggtgcggt cacagcgggt ggttttcatg
    14641 ggtagcttcc tttcaagagt tgatcaagtt tgatggcatc tgcgctgaat gcgcggatgc
    14701 cctcggctaa tttttcagtg gccatggcat cctggttgag ggcccagcga aatgctgcct
    14761 gatcgagcgt gacgtgggcg atgtcaagtg tttgcgcctg ccgggcatcg agcgcaggca
    14821 ccagtggcgc gtcgctttgc gccagcaacg ccagcagctc cgggctgatg gtcagcaaat
    14881 cgcaaccggc gagggcaatg atctggccaa cattgcgaaa actcgcgccc atgacctcgg
    14941 tcgagatgcc atattttttg tagtagttga aaatctgccg caccgacttg acccccgggt
    15001 cttgcgccag tgcgttgtca gcttccaccc aggtggtgcc ggcagacttt ttgtaccagt
    15061 cgtaaatgcg cccgacaaag ggcgaaatca actgcaccct ggcctgcgcg caggcgaccg
    15121 cctggcaaaa cgaaaacagc agggtcaggt tagtgtgaat gccgcggcgt tccagctcgg
    15181 ctgccgcttc gatgccctcc caggtggcgg ccaccttgat cagtacccgg tcaatgtgaa
    15241 tgccctgcgc ctggtaaagc tcgatgaggc gctcggcccg ggtgagggtg gcattggcat
    15301 caaaactcag gcgcgcatcc acttcggtcg aaacccgccc agggataatc gccaaaatct
    15361 cgcagccaaa acgcaccagc agccggtcca tgacttcatc aagcgcacga tcaccatgcc
    15421 tggcgaccgt ttcatccagc aaggaccggt actcgggctt ttgcaccgct ttcaaaatca
    15481 gcgacggatt ggtggtggca tcctgtggct gaaaagctgc catctgccgg aagtctccgg
    15541 tatcggccac cacggtggtg aattgtctga gggcatcgag ctggttcatg ccgtcattat
    15601 caagcgattg acgcagcccg ggtgaaacgc acgactggca ccacggcggc cagccagaca
    15661 gcgtggtccg ccagagtctg gccgacggca gcaccgaggc acaacacatc gggcaaatcg
    15721 ggtgaaacaa ccaatgttgg cctcagtgcg gcatcctatg cgcttgcaga ttgatgcggc
    15781 atcaggtgcg aagccgctgg cagcgctaca gcgcggcaag gcgaagcaac aggatgccgg
    15841 attgacatgg caatggaacc aaagg
//
LOCUS       DAOSLL010000021        15739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_3903_length_15739_cov_4.525440, whole genome shotgun sequence.
ACCESSION   DAOSLL010000021 DAOSLL010000000
VERSION     DAOSLL010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 15739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15739
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3903_length_15739_cov_4.525440"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..1728
                     /locus_tag="PLB25_01615"
     CDS             64..1728
                     /locus_tag="PLB25_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine--lipid A transferase"
                     /protein_id="HOE40324.1"
                     /translation="MPKPLSVRRPWHPTVLLMVLSLWLATAGNLPLWSAIWRLPETHG
                     WHAGLTLVSLVMAVLAVTLSVLCLLLWPRWLKPVGLLLLVIATSSSYFMQSYGVVIDA
                     TMIANVAQTDAREVYDLLSWSMLGYLLVGVLLPGLWLWQQPVRLVALGGLALRQLGLA
                     LLALLLALLLIWLSFQDLASLMRNHKSLRYMINPFNAIYAVARHGLGQAAQAQQPLQS
                     IGLDARLAPSGAAAKTSPLIVFVVGETARAANFGLGGYASDTTPRLRELQQQGDLVYF
                     SQVTSCGTNTETSLPCMFSHLGREAYQSSDARYENLLDLLQRAGLAVLWLDNQSGCKG
                     LCERVPNQVTRTLTQAGLCQDGECFDEIMLQVLPDQLARLDPQRRARGTVVVLHQMGS
                     HGPAYYKRTPATFKPFQPECTSSTLQDCQPAQIVNAYDNTLRYTDHFLSETVRWLQSE
                     ARSRPTALLYVSDHGESLGESGLYLHGMPYRIAPTEQTHVPMVVWLSAAMQRERSVRM
                     DCVREQSQKKWSHDNLFHTLLNLSQVQTSLASPTLDILAPCVAATH"
     gene            complement(1860..2234)
                     /locus_tag="PLB25_01620"
     CDS             complement(1860..2234)
                     /locus_tag="PLB25_01620"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HOE40325.1"
                     /translation="MLPKRRGLARIWYAFGYSAQGLHSGWHEPAFRQEAMLAMVALPA
                     AFWVGQTWVETALLVASLVGVMVVELLNTAVEAAIDRVGPELHDLSKRAKDLGSAAVL
                     LSLLLCGGIWACALWRLVRVWV"
     gene            2233..2502
                     /locus_tag="PLB25_01625"
     CDS             2233..2502
                     /locus_tag="PLB25_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40326.1"
                     /translation="MTGLLFKLTPSFKSDRYANTAKAWHQMARHASDRPLAVEWLKGI
                     ARQPLRSSESNAIGSSGKHLAFRFNPGKNPHGSVPLALPIAWVLT"
     gene            2688..4463
                     /locus_tag="PLB25_01630"
     CDS             2688..4463
                     /locus_tag="PLB25_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HOE40327.1"
                     /translation="MSATTQSSRNAKSLSGLLPFLKPYRSRICLALVFLLLAAMATLA
                     FPLALRSLMDGGLLQTDRGAQVMALREHFVVLFAVALALGIFSAARFYMVSWLGERVS
                     ADLRNAVYAHVLQQSPEFFETTQTGEVLSRLSADTTLVQTVVGSSLSMGLRNLVMGIG
                     ALGILVWTNPMVMAQVSAVLLLIVLPALWFGRRVRKLSRASQDRAADSSAIAAEVLNA
                     IAVVQSYTAEARETSRFTEATDRAFEVAVRRSMARSGLVAFIIIATSAALLWGLYQGT
                     QAVMAGRISAGHLGQTVVYVIILASAFAILGEVYGDLLRAAGATERLMELLASQSPIV
                     SPPNPAPALMPPEGSTIDFEAVSFHYPSRPKPPALDHFSLRIAAGQTVALVGPSGAGK
                     STVFQLLLRFYDAQSGRILLDGVPTRELALADLRQRIGIVPQEAVIFASSAFENIRYG
                     RPDASAADVKAAALAAFADEFITALPQGYDTFLGERGVRLSGGQRQRIAIARAILKNP
                     PLLLLDEATSALDAESERMVQAALESAMHGRTTLVIAHRLATVQKADRIVVVDQGRLV
                     EQGTHAELVAMGGIYAGLAALQFHA"
     gene            4537..4725
                     /locus_tag="PLB25_01635"
     CDS             4537..4725
                     /locus_tag="PLB25_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40328.1"
                     /translation="MEVSRSTNAPVQSTQKPTQLQKNEQNVQAQPQEPQTKPPAPQPA
                     PVINAQGQTTGRVLNVTA"
     gene            4751..5155
                     /locus_tag="PLB25_01640"
     CDS             4751..5155
                     /locus_tag="PLB25_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40329.1"
                     /translation="MVALAATSSATPSLQTTLMRSRLEVARREAEQAQATVDELRTEV
                     DAAETDLERRQGKVRTLTDQSSQTDPTYQPNVQTPKSAVPAKTQELLVGLYGATSAKR
                     QATGNGLKSDPNAAPVVNTRGQSTGRIVNISA"
     gene            complement(5171..9928)
                     /locus_tag="PLB25_01645"
     CDS             complement(5171..9928)
                     /locus_tag="PLB25_01645"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HOE40330.1"
                     /translation="MTNAALVSLSTYIHENSGANSHALTDFVSAYFDNTDPDELSARG
                     PAHLMAVAMAHLRLLDMLASATGGRIRVFNPTLAEDGFISEHTVIQIIQDDMPFLVDS
                     VTMVVNRSGSVAHWIVHPLLIVERGQQGQVVNTRLAGSGGPVLAQIQSFIFVECDRIV
                     REPDRAAMADELARVLGDVRAAVNDWPAMLTRLKAVREESSRHVEQTDAQLEGVEFLR
                     WLEGKHFTFLGARDYDLQRVDGTIRLVALPQSGLGILRGDPRTLVSELPADAQVSLNS
                     DQLVMVTKAMTRSTVHRPAWLDCVAVKHFDGTGAVVGESRFLGLYTSRAYAAPVAEIP
                     LVRRRIVQVMNMAGAMPDSHAAKSLQAILDAYPRDELFQIDTPTLIDHAIGILMLQKR
                     QRTRLFARRDPFGRFTSVQVFVPRDRYNTELRVRIGQELSRTLNGQSIEFTPMLTDNP
                     LARIHYLVRTVQQAPDHPDMVALEARISRLARRWEDDCSVTLMQIHGETQGLMLANRF
                     AHVFPAAYRDDFSGVVAADDALVLSGLSDACRMAVKLYRRLDAVAGEMRLKIYSTAKL
                     ALSDSLPVLERMGARVLDEHPYRLPDAEFGEFWINDLGLQLPANVEFAVVKDRFETLF
                     VQVWRGDVDNDELNKLVLQTALDARAISLLRAYTRYFKQLGFGFSQSYIEETLFRNAA
                     MAQLLFDLFNARFHPGLDTDRDAAQQAICSQIESSLVAVASLDEDRILRQFYHAILAT
                     LRTNAWQTSSSGAAKPYLSLKFNPRALPGVPEPKPWFEIWVYSPRFEGIHLRNGKVAR
                     GGLRWSDRREDFRTEILGLVKAQHVKNTVIVPVGSKGGFVLKNAPNPSDRDAYLAEGI
                     ACYRLFLAGMLDLTDNLVHGVVVPPAKLVRHDADDPYLVVAADKGTASFSDIANSVSA
                     QYGFWLGDAFASGGSVGYDHKKMGITARGAWESAKRHFRSLGIDTQTTPFTVVGIGDM
                     SGDVFGNGMLLSEQIKLVLAFDHRHIFIDPDPDLARSFAERRRLFALPRSSWADFDQN
                     VISDGGGVYARAAKSIRLSDAARALLGIDAAELAPAELLNAILKAPVDLIYNGGIGTY
                     VKSSQETHLQVGDKASDAFRVNGNELRCKVFVEGGNLGCSQNSRIEYARHGGLIYTDA
                     IDNSAGVDCPDHEVNIKILLDRVVESGDLTFKQRNDLLASMTEEVGHLVLADNYYQTQ
                     ALDVACHRPVYVLDAQQRLMQWLEGAGRLNRAIEFLPGDEEIARRRANKTGLTAPEGA
                     VVLAYAKMSVFDDLVASNLPDDPYFTRTLKAYFPKVLSEKFADTIARHPLRREIIATF
                     VTNTVVNRTGATFINFLAAEAAASTADVVRAYTLAREIFDLEPLWDQIDSLDYSVSSS
                     LQLDLLARLISISQRASRWILRLRARGADMPTLIARYQPKAAELSAHLNHWLPDHSVN
                     NWQQATQQLMQAGVAAELAQSLTALEFIFPALDLVDLAASADTTLEQAARTYFAVESR
                     LGLIAWRTQINRLPTETLWQTQARGSARDDIYAVATQITKTVLAGDQGVDDWASRHSV
                     QIDRLCQLLLSISSQTADLAPISVALRELRHLA"
     gene            complement(10159..10374)
                     /locus_tag="PLB25_01650"
     CDS             complement(10159..10374)
                     /locus_tag="PLB25_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40331.1"
                     /translation="MSLILEGRPIGWSSAMSDSEFSPGERSLTARLKGEACLLRDKLT
                     SLSMVLNDLQFERWFREKIRLLLVPIE"
     gene            complement(10381..13347)
                     /locus_tag="PLB25_01655"
     CDS             complement(10381..13347)
                     /locus_tag="PLB25_01655"
                     /inference="COORDINATES: protein motif:HMM:NF028510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HOE40332.1"
                     /translation="MNVIINTWRKAGLGSKLALSNFLLVAVALLICVVAIGYGVSRTI
                     ESRAISELTAKTNILFELIDGSDKDLKIRTANLAKAFQASLKGKIELTEASILIGDKT
                     TPVVKLDGKSVNLDFALVDRFTEATSAVATVFARTGDDFVRITTSLKTDKGERAVGTL
                     LDRNHPGYKAVTGGGSYTGLAMLFGRQYMTQYDPIRDSQGKVIGLSFVGLDFTDYLTS
                     LKATILKIKIGESGYFYVLDARPGDSYGKLIIHPVSEGKNIIASKDTSGREFIKEILD
                     RKTGIIRYPWINKDLGEASPREKVVSFTHLASWNWVIAGGTYVEEFTAEINKLRNSIA
                     LAGLVIVVAISAGWLVLIRRIVIQPNGEVSATAEKIAQGDMSSELETDRQDEIGRLVG
                     SMSKMQSVLTQFQSGLSEMSAKHSAGQLDHVIPTNNLPGAYGDIAKAINDMVQSHIAI
                     KMKIVDVITDYSEGKLDVAMDRLPGQKARISEALDKVQATMKEAASAARTNLRIRQAL
                     DKCTTNVMIANADNQIVYMNETVQAMMQRNEAELKKTLPQFDARHLVGQNMDMFHKNP
                     AHQRNMLAQLTSVYKTQIQVSDLYFSLSANPIIDEQTIRVGTVVEWADRTAEVGVEKE
                     VASIVASAAHGDFSHRLDMAGKTGFFANLSSNMNQLLDTSEQGLNDVSDVLAAFAAGD
                     LTKRMDRAYEGLFGKVKLNVNSTAENLTRVIGEVHSAADALTGAANQVSATAQSLSQA
                     ASEQAANVEETSSQIDVMSASISQNSDNARITDGMATKTSKEAVDGGIAVSQTVAAMK
                     QIAAKIGIVDDIAYQTNLLALNAAIEAARAGEHGKGFAVVAAEVRKLAERSQEAAKEI
                     GDLADSSVSTAERAGKLLEEIVPSIQRTSELVQEISSASSEQSESVNQIGGAMGQLSK
                     ATQQNASASEQLAATSEELSGQAEQLQQSIAFFNTGEEAPKVRSRHEAPALERMASVP
                     RLTAKSVPRGSNFRPY"
     gene            14051..14809
                     /locus_tag="PLB25_01660"
     CDS             14051..14809
                     /locus_tag="PLB25_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40333.1"
                     /translation="MNINDFVTANSASNYLGLNSSSRAATGVSATGQNSLARAEKRIQ
                     SQVDVTTAQLSSLGKLKSAVSGTQIAAQALGKLSATSTNAGAKTAAESFVTAFNSAIG
                     TAKTASEATGEPSSSLSASRVGRDLVRTVSADTSTRDALKKIGFNLTSDGKLTLDASK
                     FAAAQTGDAAAVNATLAKIGQQVDQTATRELASTGNVGTAVTSLNQRASVLKNQQSAL
                     ATLQQTTPGAADNSAYSNAFSGFGNLGLSAYKNY"
     gene            complement(15049..>15739)
                     /locus_tag="PLB25_01665"
     CDS             complement(15049..>15739)
                     /locus_tag="PLB25_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOE40334.1"
                     /translation="RRKLLLLGMLNSGLPFASISFALLSISTGLSSLLNATVPLFGAL
                     VAWLWLKDRPHGLKIIGLLIGFVGVAMLAWGKASFKPDASGHASGWAVLACLFACLCY
                     GISASYTRRYLAGVPSLVIATGSQIGAAVGLCLPTFWLWPERNPGITAWLALLAVGVL
                     CTGIAYVLFFRLIEKMGAAKSLTVTFLIPVFAVIYGAVFLDESVTSWMLLCGAVILFG
                     TAMSLDLFKRR"
BASE COUNT         3358 a         4628 c         4478 g         3275 t
ORIGIN      
        1 ataagactgg aacaaagtcg ggcaattaaa attgccggtt tgccaactca ggattcatgc
       61 cacatgccca agccgctttc ggttcgccgc ccctggcacc cgaccgtcct gttgatggtg
      121 ctgtccttgt ggctcgccac agcaggcaac ctgcccttat ggagcgccat ctggcgcttg
      181 cctgaaacgc atggctggca cgctggcctg acgctggtca gtctggtgat ggccgtgctg
      241 gcggtcacgc tgtcggtgtt gtgcctgctg ctttggccac gctggctcaa gccggtcggc
      301 ctgctgctgc tggtgattgc cacctcaagc agctacttta tgcagagcta cggtgtggtg
      361 attgatgcca ccatgatcgc caacgtggca cagaccgatg cgcgcgaggt gtacgacctg
      421 ctgtcctggt cgatgttggg ctatttgttg gtgggcgtgc tgctgccggg cctgtggtta
      481 tggcagcagc cggttcgact ggtcgcactc ggcggcctgg cgctgcgcca gctcgggctg
      541 gcactgctgg cactgctgtt ggcattgctg ctgatatggc tgtcttttca ggatctggca
      601 tcgctgatgc gcaaccacaa gtccttgcgc tacatgatca acccgttcaa cgcgatttat
      661 gccgttgcgc ggcatgggtt gggacaggca gcacaggcgc agcagccatt gcagagcatt
      721 ggactggatg cccggttggc cccatccgga gcggcggcaa agacctcgcc actgatcgtt
      781 tttgtggtgg gcgaaaccgc ccgggctgcc aactttggac tcggtggcta tgcgagcgac
      841 accactccca gactgcgcga actgcagcag cagggcgatc tggtgtactt cagccaggtg
      901 acttcgtgcg gcaccaacac cgagacctcg ctgccctgca tgttctcgca tctggggcgt
      961 gaggcttacc agagtagcga cgcgcgctat gaaaacctgc ttgacctgct gcaacgcgct
     1021 ggcctggcgg tgctctggct ggacaaccag tccggctgca aggggctgtg cgagcgggta
     1081 cccaatcagg ttacccgcac gctgacacaa gccggtttgt gccaggacgg cgagtgtttt
     1141 gacgagatca tgctgcaggt gctgccagac cagttggcgc ggctcgaccc gcagcgccgg
     1201 gcgcgcggca cggtggtggt attgcaccag atgggcagcc acggcccggc ctattacaaa
     1261 cgcacaccgg ccacgttcaa gccatttcag cccgaatgca ccagcagcac cttgcaggat
     1321 tgtcagcccg cgcagatcgt caatgcttac gacaacaccc tgcgctacac cgaccacttc
     1381 ctcagcgaga cggtgcgctg gcttcagagc gaggccaggt cgcggccaac tgccctgctg
     1441 tacgtatcgg accatggtga atcactgggg gaaagcgggc tttacctgca cggcatgcct
     1501 taccgtatcg cacccactga gcagacccat gtaccaatgg tggtgtggct gtcggctgcc
     1561 atgcagcgcg aacgcagcgt gcgcatggac tgtgtgcgcg agcagtcgca aaagaagtgg
     1621 tcccacgaca acctgttcca caccctgctg aacctgtcgc aggttcaaac cagcctggca
     1681 agcccgacgc ttgatatcct cgcgccttgc gtcgccgcaa cccattaggc gaggggctca
     1741 ttgcgtttac agatcgatgc gacattgggc gcgacgccgc agacagtgcg acagcacggc
     1801 aaggccaggc caggccaggc aacaacatgg cgacatgtcg gatggattcg gaaatgcaat
     1861 cagacccaaa ccctgaccaa gcgccacagc gcgcaggccc agatgccccc gcacaacaac
     1921 aggcttagca gcaccgccgc gctgcccagg tctttggccc gcttggacag gtcgtgcagt
     1981 tcggggccga cccggtcaat ggcggcttca acggcggtgt tgagcagctc aaccaccatc
     2041 acgccgacca ggctggcaac cagcagcgcg gtttctaccc aggtctggcc gacccagaaa
     2101 gccgctggca gggcaaccat cgccaacatc gcttcctggc gaaaagccgg ctcgtgccag
     2161 ccagaatgca gaccctgggc agagtagcca aaggcatacc agatgcgcgc caaacctcga
     2221 cgctttggca acatgaccgg attgctgttc aagttaactc caagtttcaa aagtgaccgc
     2281 tatgctaata cagccaaggc atggcaccag atggcgcgcc atgcgtccga ccggcccctg
     2341 gcggtggagt ggttgaaggg catcgcgcgg caacccttgc gttcgtccga atcaaatgcc
     2401 atcggcagtt caggcaagca cctggctttc cgatttaacc cagggaaaaa tccgcatggg
     2461 tcagtgcctt tggcgttacc catcgcttgg gtgttgacgt aaaaccgggt gcagcaggct
     2521 cggcccgact cgccccgcag agataaagta gctatatttt ttatagctac ttgggcaata
     2581 cctgcaaggg ctagagtcct ttttttcata aagtccggaa tgcctctgct gatctgatac
     2641 cgttcgaccg gtacgctgcc ctgccctttc ccatcacctg tgtgtttatg tctgccacca
     2701 cccaatcatc acgcaacgcc aagtcgctgt ccgggctact gccttttttg aaaccctacc
     2761 gctcccgcat ctgcttggcg ctggtgtttc tgctgctggc cgccatggcg accctggcct
     2821 ttccgctggc gctgcgcagt ctgatggatg gtgggctgct gcaaaccgac cggggtgcgc
     2881 aggtgatggc gctgcgcgag cactttgtcg tgctgtttgc cgtcgcgctg gcgttgggaa
     2941 ttttttcggc ggcacgtttt tacatggtga gctggctcgg cgaacgcgtc agtgccgacc
     3001 tgcgcaacgc cgtctatgcc catgtgctgc agcaaagccc ggaatttttc gagaccaccc
     3061 agactggcga ggtgctctct cgcctgtcag ccgacaccac gctggtgcaa accgtggtgg
     3121 gctcgtccct gagcatgggt ctgcgcaacc tggtgatggg catcggggca ctgggcattc
     3181 tggtctggac caatccgatg gtgatggctc aggtgtcggc ggtgctgctg ctgatcgtgc
     3241 tgccagccct gtggtttggc agacgtgtgc gcaagctctc gcgggccagt caagaccgtg
     3301 cagcagactc cagcgcaatt gccgccgagg tgctcaatgc gattgcggtg gtgcaaagct
     3361 acaccgccga agcgcgggaa accagccgtt ttaccgaggc cactgatcgc gcctttgaag
     3421 tcgcggtgcg tcgctcaatg gcccggtctg gcctggtggc cttcatcatc attgccactt
     3481 cggcagcgct gctgtggggt ttgtaccagg gcacgcaggc ggtgatggcc ggtcgcatca
     3541 gcgccggcca tttgggtcaa acggtggtct acgtgatcat tctggccagt gcgtttgcga
     3601 ttctgggtga ggtctatggc gacttgctgc gcgccgccgg ggcaactgag cgactgatgg
     3661 aactgctggc cagccagtca cccattgttt caccaccaaa tccggctcca gcccttatgc
     3721 cacctgaagg aagcactatt gattttgaag ctgtttcatt tcattaccca tcgcgaccga
     3781 aaccgccagc gctggaccat ttttcgctgc gcatcgccgc cgggcagacg gtggcgctgg
     3841 tgggacccag cggcgcgggc aaaagcacgg tgtttcagtt gctgctgcgc ttttacgatg
     3901 cgcaaagcgg gcggattttg ctcgacggcg tgcccacccg tgaactggcg ctggctgatt
     3961 tgcgccagcg catcggcatc gtgccgcagg aggcggtgat ttttgccagc agcgctttcg
     4021 agaacatccg ctacggtcgg ccagatgcca gcgcggctga cgtcaaggcg gccgcgctgg
     4081 cagcctttgc cgatgagttc atcaccgcct tgccacaggg ttacgacacc tttctgggcg
     4141 aacgcggtgt gcgtctgagc ggcgggcagc gccagcgcat tgcgattgcc cgcgccatct
     4201 taaaaaatcc gccgctgctg ctgctcgacg aagccaccag cgcactcgat gccgaaagcg
     4261 aacgcatggt tcaggccgcc ctcgaatctg ccatgcatgg ccgcaccaca ttggtcatcg
     4321 cccaccgcct ggcgacagta caaaaggccg accgcattgt ggtggtggat cagggacggc
     4381 tggtggagca aggcacccac gccgaactgg tggccatggg cggcatctat gccggtttgg
     4441 ccgctttgca gttccacgct tgagggtttg ccgtgacggg ttgaatattg aacgcaagac
     4501 tcgtaaactt gcaaatgtcc ttgaaaggag tcccaaatgg aagtttcacg ctccaccaac
     4561 gcaccggtgc agtccaccca aaagccgacg cagttgcaaa aaaatgagca aaacgtgcag
     4621 gcgcaacccc aggagccaca gaccaaaccc ccagcgccac agcctgcacc ggtcatcaac
     4681 gcacaagggc agacgaccgg acgggttttg aatgtgacgg cctgaggtca gtgctgtagc
     4741 aggtgtcatc atggttgccc tagccgccac cagcagtgcc acaccgtcgc tgcaaaccac
     4801 actgatgcgc agtcgtcttg aagtggctcg tcgtgaggca gagcaggcgc aagcaacagt
     4861 ggacgaactg cgcacagagg ttgacgcagc ggagactgac cttgaaaggc gtcagggaaa
     4921 agtgcgcacc ctgaccgatc agtccagcca gaccgacccg acttatcagc ccaatgtgca
     4981 gactccgaaa tctgccgtgc cagccaaaac ccaggagctt ttggtcgggc tttacggtgc
     5041 taccagtgcc aagcgccagg cgaccggaaa tggcctgaaa agcgatccca acgccgcccc
     5101 tgtggtcaac acaagggggc agtctaccgg acgaatcgtc aacatcagcg cctgaagcat
     5161 cgccttgcca tcacgcaaga tggcgcagtt cgcgcagcgc taccgaaatc ggtgccaggt
     5221 ccgctgtctg gctgctgata ctgagcagca actggcacag gcggtcaatt tgcactgaat
     5281 gtcggcttgc ccagtcatca accccttgat cgcccgccaa caccgttttg gtgatttgtg
     5341 tggcaacggc atagatatca tcgcgagcgc tgccacgcgc ttgcgtttgc cacagggttt
     5401 cggtcggcaa gcggttgatt tgtgttcgcc aggcaatcag ccccaggcgg ctctcaacgg
     5461 caaaataagt tcgggcggcc tgctccagcg tggtgtcggc gctggcagcc aggtcaacca
     5521 ggtccaacgc cggaaagatg aactccagcg cggtcaggct ctgcgccaac tcagcggcca
     5581 cgccggcctg catcagttgc tgggtcgcct gctgccagtt gttcaccgaa tgatcgggta
     5641 gccagtggtt caaatgagcg ctcagctctg ccgctttggg ctgatagcgt gcaatcagcg
     5701 ttggcatgtc ggcccccctg gcacgcaagc gcaggatcca gcgcgaggct cgctgcgata
     5761 tcgaaatcag gcgggccagc agatcgagtt gcagcgagct tgagacactg tagtcaaggc
     5821 tgtcgatctg gtcccacaag ggttcaaggt caaaaatctc acgcgccagc gtataggcac
     5881 gcaccacatc agcagtgctg gccgcagcct ctgccgccag aaagttgatg aaggtggccc
     5941 cagtccggtt gaccaccgtg ttggtcacaa aagtggcaat gatttcacgg cgcaacggat
     6001 ggcgcgcaat ggtgtcggca aacttttccg acaagacctt ggggaaatag gccttgagtg
     6061 tgcgggtgaa atacggatca tctggcaggt tgctggcaac caggtcatca aacacggaca
     6121 tcttggcata ggccagaacc accgcaccct cgggtgccgt caaaccggtc ttgttggcgc
     6181 ggcgacgggc gatttcctcg tcgccgggca gaaattcgat agcgcggttc agacggccag
     6241 caccttcgag ccattgcatc aaacgctgct gggcatcgag cacatacacg ggccgatggc
     6301 aggccacatc aagcgcctgg gtctggtagt agttgtctgc cagcaccaaa tggcccactt
     6361 cctcggtcat ggaagccagc agatcgttgc gctgcttaaa ggtcaggtcg ccagattcca
     6421 ccacccgatc aagcagtatc ttgatgttca cctcgtggtc cgggcagtcc acgccagccg
     6481 agttgtcgat cgcatcggtg taaatcaggc caccatgccg ggcatattca atccggctgt
     6541 tctggctgca gccaaggttg ccgccttcga caaacacttt gcaacgcagt tcgttgccat
     6601 tgacccggaa agcgtcgctg gccttgtcac cgacttgcag gtgggtttcc tgcgaagact
     6661 tgacgtaagt gccaatgccg ccgttgtaaa tcaggtcaac cggtgctttc aggatggcat
     6721 tgagcaattc tgccggggcc agctcggccg catcgatgcc aagcagcgcg cgtgccgcat
     6781 cagatagccg gattgatttg gcggccctgg cataaacacc accgccgtcc gagatgacat
     6841 tttggtcaaa gtcagcccaa ctcgaccgtg gcaatgcaaa cagccgacgg cgctcggcaa
     6901 aactgcgtgc aagatccggg tcagggtcaa tgaagatgtg ccggtgatca aatgccagca
     6961 ccagtttgat ttgctctgac agcagcatgc cgttgccaaa cacatcgccc gacatatcgc
     7021 caatccccac cacggtgaag ggggtggttt gggtatcaat gcccagcgac ctgaaatggc
     7081 gcttggcgga ctcccaggcg ccgcgtgcgg tgatgcccat ttttttatgg tcatagccaa
     7141 ccgagccgcc agacgcaaaa gcatcaccca gccagaaacc atattgagcc gacacgctgt
     7201 tggcaatgtc cgaaaagctg gcggtgccct tgtcggctgc caccaccaga taagggtcat
     7261 cggcatcgtg gcgcaccagc ttggccggtg gcacaacgac accgtgtacc aagttatcgg
     7321 tcaggtccag catgcctgcc aaaaacaggc gatagcaggc gatcccctcg gccaggtaag
     7381 cgtcgcggtc acttgggttg ggggcattct tgaggacaaa cccgcctttg gagccaaccg
     7441 gaacgatcac ggtattcttc acatgttgcg ccttgaccag tcccaaaatc tcggtgcgga
     7501 agtcttcgcg ccggtctgac cagcgcaggc cgccacgggc cactttgccg ttgcgcaggt
     7561 ggatgccctc aaagcgtggc gaataaaccc aaatttcaaa ccagggtttg ggctcgggaa
     7621 caccgggcaa tgcacgcgga ttgaacttca aactcaagta aggcttggca gcaccgctgc
     7681 tactggtctg ccaggcgttg gtgcgcaatg tcgccaggat tgcgtggtag aactggcgca
     7741 ggatgcggtc ctcatcaaga ctggcaaccg ccaccaggga cgactcgatt tgactgcaaa
     7801 tcgcctgctg cgctgcgtca cggtccgtgt cgagccccgg atgaaagcgc gcattgaaca
     7861 aatcgaacaa aagctgggcc atcgcagcgt tgcgaaagag tgtctcttcg atatagctct
     7921 ggctgaaccc aaagccaagc tgtttgaaat agcgggtgta agcacgcagc aaggaaattg
     7981 cccttgcatc cagtgcggtc tgcaacacca gcttgttcaa ttcatcgtta tccacatcgc
     8041 cacgccagac ctgaacaaac agggtttcaa agcgatcttt gaccacggcg aactcgacgt
     8101 tggcaggcaa ctgcaagccc agatcattga tccagaattc gccgaactca gcgtcaggca
     8161 gccggtaagg atgttcgtcc agcacacggg cacccatgcg ttcaagtacc ggcaaagagt
     8221 cagacaaagc gagcttggcg gtgctgtaga tcttcaatct catttcgcca gccactgcgt
     8281 caagcctgcg gtagagtttg accgccatcc ggcaggcatc cgacagacca gagagcacca
     8341 aggcatcgtc cgctgctacc acgccggaaa agtcatcccg atacgcggca ggaaaaacat
     8401 gcgcaaagcg attggccaac atcagaccct gggtttcgcc atgaatctgc atcagggtaa
     8461 cgctgcaatc gtcctcccaa cgccgcgcca ggcgcgagat gcgcgcctcc agggcgacca
     8521 tgtcaggatg gtccggcgct tgctggacag ttcttaccag gtaatgaatg cgcgccagcg
     8581 ggttgtcggt cagcatgggc gtgaattcga tcgactggcc attcagcgtg cggctcagtt
     8641 cctggccgat ccgcacgcgc agttcggtgt tgtagcggtc acgcgggaca aacacctgca
     8701 ccgaagtgaa gcgcccaaag gggtcgcgtc tggcaaacag gcgggtgcgc tggcgctttt
     8761 ggagcatcaa gatgccaatc gcatggtcta tcaaggttgg tgtgtcgatc tgaaacaact
     8821 catcacgtgg ataggcatcc agaatcgcct gcagggattt ggcggcgtga ctgtcgggca
     8881 tggcaccagc catgttcatg acctgaacaa tgcgtcggcg taccaaggga atttctgcaa
     8941 caggtgcagc gtaggccctg gatgtgtaaa gcccgagaaa tcgcgattct cctaccactg
     9001 cgccagtgcc gtcaaagtgc ttgacggcaa cgcagtcgag ccaggcgggc cgatgcaccg
     9061 ttgagcgggt catggctttg gtgaccatca ccaactggtc tgagttcaat gaaacctgcg
     9121 catcggcagg cagttcgctg accagcgttc tggggtcacc gcgcaagatt cccaggccgc
     9181 tttgcggcaa ggccaccagc ctgatggtcc catcgacgcg ctgcaagtcg tagtcgcgcg
     9241 cacccaggaa agtaaagtgc ttgccttcaa gccagcgcaa aaactcgacc ccctccaatt
     9301 gagcatcggt ttgctcgacg tgtcgcgatg attcctcgcg gaccgctttc aggcgggtca
     9361 gcatggcggg ccaatcgttc acagcggctc tcacatcgcc caggacgcgc gccagctcat
     9421 ccgccatcgc cgcccgatca ggttcgcgca cgatacgatc acactcgaca aagataaagg
     9481 actgaatctg tgccagcacc ggtccgcctg acccggccaa ccgggtgttg acgacctgcc
     9541 cttgctgacc acgctcaaca atcagcaagg gatgaacgat ccaatgggca acgctgccgc
     9601 tgcggttgac caccatggtg acggaatcca ccaaaaacgg catgtcgtcc tgaatgatct
     9661 ggatcaccgt gtgttcactg atgaacccat cttcagccag cgtcggattg aacacccgga
     9721 ttctgccgcc ggttgcgctg gccagcatgt ccagcaacct caagtgggcc atggcgacag
     9781 ccatcaaatg ggctgggcca cgagccgata gctcatcagg gtcagtgttg tcaaaataag
     9841 cagacacaaa gtcagtcagc gcatgactgt ttgcgcctga gttctcgtga atgtaggtac
     9901 tcaggctcac aagtgcggca ttggtcatgc gggtctcctt atgttctttc gcaagaaatt
     9961 cattttaggt atcaagctga ggaagaaggg aacatattga atcttggccg cccgcatcaa
    10021 tccaattgca ttgcaacatc agcttcagac tgcctgagcc gaccttttga tctggggcga
    10081 ggcaaactct ccgcctcctg gggcgcttga aaaacgacag tgtccgtagg gcgctctgga
    10141 acaaggacaa gccgctggtc attcaatcgg caccaacaac aacctgatct tctcccgaaa
    10201 ccaacgctca aattgcaagt cgttgagcac catggataga ctggtcagtt tgtcgcgcaa
    10261 taggcatgct tcacctttca atcgagcagt caaacttcgc tcaccaggac taaattctga
    10321 atcagacatg gcagatgacc atccaatggg acggccttca aggatcaggg acatcacatt
    10381 tcaatacggc cggaaatttg atcccctggg aactgacttt gccgtgagtc tgggcactga
    10441 tgccattcgc tctagcgctg gggcttcatg ccggcttcgt accttgggag cttcctcacc
    10501 cgtattgaag aaggcgatcg actgctgcaa ctgctcagcc tggccagaca actcttcgct
    10561 ggtcgctgcc aactgctctg aggccgaggc attttgctgt gttgccttgc tgagttgccc
    10621 catggcacca ccaatttgat tgacagattc gctctgttcg ctcgacgcac tcgagatttc
    10681 ctgaaccaat tcactggttc gttggatgct tggaacaatc tcttcgagca gtttgccagc
    10741 acgctcagcc gtcgacacac tgctgtcagc caagtctccg atctcttttg ccgcttcctg
    10801 gctgcgttca gcaagtttcc tgacctcggc tgcgaccacc gcaaagcctt taccatgttc
    10861 tcctgctcgt gccgcctcaa tggctgcgtt gagcgccaat aggttggtct ggtaagcaat
    10921 gtcatccaca atgccaatct tggcggctat ttgcttcatg gcagcgaccg tctgactgac
    10981 cgcgatgccg ccatcaacag cctctttgga ggttttggta gccatcccgt ccgtgattcg
    11041 tgcgttgtcg ctgttttggc taatggacgc tgacatcacg tcaatttgtg aagaggtttc
    11101 ctccacattg gcggcttgtt cactggcagc ttgcgataag gattgcgcag tggcgctgac
    11161 ctggttggcg gctccggtca acgcatcggc tgctgaatgc acctcgccaa tcacacgcgt
    11221 gagattctcg gcggttgagt tgacattgag cttgaccttg ccaaacagtc cctcgtaggc
    11281 gcgatccatg cgtttggtca ggtcgccagc ggcaaaggca gccagaacat ccgaaacgtc
    11341 gttcaatcct tgctcactgg tatcgagcaa ctggttcatg ttgctggaaa ggttggcaaa
    11401 gaaacctgtt ttgccggcca tgtccaaccg gtggctaaag tccccatgcg ctgccgacgc
    11461 gacaatcgat gcgacctcct tctccacacc aacttcagcc gtacggtcag cccattcaac
    11521 cactgtgcca acgcgtatcg tttgctcgtc aatgatcggg ttggcactta acgagaagta
    11581 caggtcgctg acctgaatct gagtcttgta cacacttgtc aactgggcca gcatattgcg
    11641 ctggtgtgcc gggttcttat gaaacatgtc catgttttgc cccaccaggt gtcgtgcgtc
    11701 aaactgcggc aatgtctttt tcaactcagc ttcgttgcgc tgcatcatgg cttgcaccgt
    11761 ctcgttcatg taaacgatct ggttgtcggc attggcaatc atcacattgg ttgtgcactt
    11821 gtccagcgcc tggcgaattc gcaggttggt gcgcgcggcg cttgcagctt ctttcatggt
    11881 tgcctgcact ttgtccagtg cttcgctgat tctggctttc tggccaggca agcggtccat
    11941 cgccacgtcg agtttgcctt ctgaatagtc agtgatcaca tccacgattt tcatcttgat
    12001 ggcgatatgg gactggacca tgtcgttgat ggctttggca atatcaccat aggcaccggg
    12061 aaggttgttg gttggtatga cgtggtcgag ttgtcctgca ctgtgtttgg ctgacatttc
    12121 tgacaggcca gactgaaact gggtcaaaac ggactgcatc ttgctcatcg acccgaccag
    12181 tcgcccaatt tcgtcctgac gatcggtttc cagctcggaa gacatgtcgc cttgggcaat
    12241 tttctctgct gttgcactga cttcaccatt gggttgaatg actatgcgac gaatcaaaac
    12301 cagccagccc gccgagatcg ccacaacaat gaccaagccc gccaaggcaa tcgagttgcg
    12361 caatttgttg atttctgcgg tgaactcttc gacataggtt ccacctgcaa tcacccagtt
    12421 ccagctcgca aggtgggtaa aggacacaac cttttcgcgc ggactggcct cacccaagtc
    12481 tttgttgatc cacgggtagc gaatgattcc tgtctttcga tccaggattt ccttgatgaa
    12541 ctcccgcccg ctggtgtctt ttgatgcaat gatgttcttg ccttcactga cgggatggat
    12601 gatcagcttg ccatagctat ctcccgggcg ggcatcgagc acataaaaat accctgactc
    12661 gccaatcttg attttgagaa tggtggcctt gagtgaggtc agatagtcgg taaagtcaag
    12721 tccgacaaag ctcaagccaa tcactttgcc ctgtgaatca cgaatcgggt catactgggt
    12781 catgtactgc cgaccaaaca gcattgccaa accggtgtaa ctgccaccac cagtcacagc
    12841 cttgtagccg ggatgatttc ggtccagcag cgtgccaacg gcccgttctc ccttgtcggt
    12901 tttcagcgaa gtggtgatgc gcacaaaatc gtccccggtc ctggcaaaca cggtggcgac
    12961 ggcagaagtg gcttcggtaa agcggtcaac cagtgcgaaa tcaaggttga cagatttgcc
    13021 atcaagcttg accaccggtg tggttttgtc accgatgagg atgctggcct cggtcagctc
    13081 tatttttcct ttgaggctgg cctggaacgc cttggccagg ttggctgtgc gaattttcaa
    13141 atccttgtct gacccatcaa tcaactcaaa caagatgttc gtcttggcag tcagctcgga
    13201 aattgccctg gactcaatcg tccgggaaac gccatatccg atggctacaa cgcagatcag
    13261 aagtgccact gcaaccaata gaaaattgct caatgccagt ttggacccca agcctgcttt
    13321 gcgccaggtg ttgatgatga cgttcatgat gctttcgtga tgacagggaa gaactggtgg
    13381 gtgatcgcgg tgtcgcaact gccataagcc gcgtgatgaa accgcgttga agtggacttg
    13441 ccaatgaata tcggtggaat ggacgaatca cgattcaaga cagggcgaat ctgattgcac
    13501 aaagctggta tcaaatacgc atgattgtca gtatttcaga ctgctgaatg ttgcgataga
    13561 tcaactttgg caagatttgg gaggcttttg tgacccattc agtgcaggct caattgatat
    13621 gcgactttca gttgcataac gtttggaaaa cagccttgaa tggcttgggc tacctgcagg
    13681 gtagccgggg gtcgcgcacc ccatatttgt ttgaaaatgc caagtcagaa cgccatgcgt
    13741 tgccgtactg gtcgcactgg gtatgtactt cgacggcatc ttgcgcgaca tcgttcgtca
    13801 gaccaatgta tgcctcgggc cttgggtgcg tcctcatcaa agcggccatc gccgagcact
    13861 tcacgcagga tgggttggat tgttcgcgtg acaattctgg cattgctgcg atggcgggct
    13921 gacagcaatt caatggcttg acgaatgagc gaattctctt tgtccggaca ccaggaaatc
    13981 gacacctttt tgctgttcag gattaaactt caatgcgagt tctgtcgagc aaattcgaaa
    14041 aaggcgcatc atgaacatca acgactttgt tactgccaat tcagccagca attacctggg
    14101 gctcaacagt tcttcgcgcg ctgccaccgg tgtcagcgcg accgggcaaa acagcttggc
    14161 aagggcagaa aagcgcatcc agtcgcaggt ggatgtgacc acagcccagt tgtcgtcttt
    14221 gggcaaactc aagtctgcgg tatcgggcac gcagatcgct gcccaggcgc tgggcaaatt
    14281 gtcggctacc agcaccaatg ctggggccaa gactgcggca gagagttttg tgactgcctt
    14341 caactcggcc atcggcaccg ccaaaacagc ttctgaggcc accggcgagc catcatcttc
    14401 cctgagcgcg agtcgggttg gcagagatct ggtgcgcacg gtatcggccg acaccagcac
    14461 ccgcgatgcg ctgaaaaaga tcggcttcaa tctgaccagt gatggcaagt tgacgctcga
    14521 tgccagcaag tttgctgcag cgcagacggg tgatgcggcc gcagtcaacg cgaccctggc
    14581 caaaattgga cagcaagtgg accagacggc cacccgtgag ctggcctcaa ccggcaatgt
    14641 cggcaccgcc gtcacgtcgt tgaaccagcg tgcctccgtc ctcaagaacc agcaaagtgc
    14701 gctggccaca ttgcagcaaa cgacacccgg agccgccgac aacagcgctt acagcaacgc
    14761 cttctcaggc tttggcaacc ttggcctgtc ggcttacaag aactattgag catcccaacc
    14821 ccgcaagcgg ggcaggcgtt ctcaaaggaa catgggctag gccgatggcg caagctgccg
    14881 gggtttcaac tgccaaatcg gcatccagcc cttatgcaga ctgcgttgcc agctatattt
    14941 ttgattgtta acacagtatc tgtttcttag gaatggcctg accccaaacg ctgcgtgatg
    15001 ccgggcttgc caggtggccc ctgactgcag gcggttaccg ccgctcgttt accgccgctt
    15061 gaacaaatcc agcgacatgg ccgtgccaaa caggatgacc gcgccgcaca gcagcatcca
    15121 cgaggtaacc gactcgtcca gaaagacggc accatagatc actgcaaaca ccggaatcaa
    15181 gaacgttacc gtcagcgact tggcggcccc cattttttct atcaaccgga aaaacagcac
    15241 ataggcgata ccggtacaca gcacgcccac cgccagcagc gccagccagg ccgtgatacc
    15301 ggggttacgc tcgggccaaa gccagaaagt tggcaagcac agccccaccg cagcgccgat
    15361 ctggctgccg gtggcaatca ccagcgacgg cacgccagcc aggtaacgcc gggtgtaact
    15421 ggccgaaata ccgtaacaca ggcaggcaaa caggcaggcc agcacggccc agccgctggc
    15481 gtggccagag gcatcgggct tgaaactggc tttaccccag gccagcatgg cgacaccgac
    15541 aaagccgatc agcaggccga tgattttcag gccgtgtggc cggtctttca gccaaagcca
    15601 ggccaccagc gcaccgaaca gcggaacggt ggcattcagc agtgaagaca agccggtgct
    15661 gatggacagc agcgcaaacg agatactggc aaacggcagg cccgagttca acatgcccag
    15721 cagcagcagc ttgcggcgg
//
LOCUS       DAOSLL010000022        15450 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_3982_length_15450_cov_6.290354, whole genome shotgun sequence.
ACCESSION   DAOSLL010000022 DAOSLL010000000
VERSION     DAOSLL010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 15450)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15450)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15450
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3982_length_15450_cov_6.290354"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="PLB25_01670"
     CDS             complement(<1..1351)
                     /locus_tag="PLB25_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE40335.1"
                     /translation="MTISKTTTPHKFAHKRTALCVLALPALLALSPVSAKTLVYCSEG
                     SPENFAPSINTTGTSFDASEQIYDNLVDFERGGTKVVPGLAESWTVSKDGLEYVFKLR
                     KGVKWQSNKNFKPTRDFNADDILFMFERQWKENDPYFKVTSSNHAYFGDMGMPKLLKS
                     VDKVDDYTVRIVLNAPEAPFLSNLAMQYAGVQSKEYAIAMLKAGTPEKIDQEPVGTGP
                     FMLVQYQKDAIIRYKANPVYWAGKAKIDDLIFSITPDASVRWAKLQKGECHVMPYPNP
                     ADLDAIRKDPNVTVLEQPGLNIGYLAYNTTKKPFDDVRVRKAVNMAIDKKAIVAAVYL
                     STGVPATNPIPPTQWSYNKAIKDDAFDPAAAKKLLAEAGLPNGFSTDLWAMPVQRPYN
                     PNAKRIAELMQADLAKVGIKAEIKSFEWGEYRKRMQAGEHQMGMLGWTGDNGDPDNFL
                     "
     gene            complement(1371..2396)
                     /locus_tag="PLB25_01675"
     CDS             complement(1371..2396)
                     /locus_tag="PLB25_01675"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HOE40336.1"
                     /translation="MSQPHKPSDPSVEPVVLARDLKQVYKISHGAFRKADHLQAVSGV
                     SFSINPGKTLAVVGESGCGKSTLARMVALIEAPTSGQLALGKVDVVGASTQQRQALRR
                     TVQLVFQNPFGSLNPRKKIGAILQAPLEINTDLGADERAEQARAMLALVGLRPEHFDR
                     YPHMFSGGQRQRIAIARALMLKPGLIVADEPVSALDVSIQAQVLNLLADLQHELGLAY
                     LFISHDLGVVRHIAHDVMVMYLGHAVEHGDKKTLFAQPLHPYTQALLASTPGIAGSGV
                     SRQRIVLKGELPSPLNPPSGCVFSTRCPHVTPQCQSERPVLRELAGRQLACHLAEQFV
                     QPAAAQH"
     gene            complement(2393..3382)
                     /locus_tag="PLB25_01680"
     CDS             complement(2393..3382)
                     /locus_tag="PLB25_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE40337.1"
                     /translation="MALLEIENLSVQFPSKTAIMHAVDGVSMTLQAGDVLGIVGESGS
                     GKSVTMMALMGLVAYPGRISADKLHFDGHDLLKLSERERRKLTGKDLAMIFQDPTTSL
                     NPCFTVGFQMAETLQLHLGLDKAAARKRSIELLEQVGIPAPESRLGIYPHQLSGGMSQ
                     RVMIAMAIACNPKLLIADEPTTALDVTIQAQILDLLRNLQKERNMALVLITHNMGVVS
                     EMAQRVAVMYAGQIMEERSAHDIFEAPQHPYTEALMAAMPERSDGLTRLATIPGMVPG
                     LYDRPGGCLFAPRCSYVTQHCKDQRPALREWQSGMVRCHYPLESGVAASGAPA"
     gene            complement(3393..4286)
                     /locus_tag="PLB25_01685"
     CDS             complement(3393..4286)
                     /locus_tag="PLB25_01685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE40338.1"
                     /translation="MTTLTESPAQAPQHPLREFWDYFQTNKGAVAGLLIVLAVLLMAA
                     FAPLIAPYPPDATDSSVFLTPPAWQAGGSSAHWLGTDAIGRDILSRLIHGARLSLAIG
                     LAVVALSVIAGTVLGLTAGYFRGIYEVAVMRLMDIILTLPSLLLAIVIVAILGPGLMN
                     AMLAVAIVVLPHYVRITRAAVISEMSRDYVTAARMNGAGHLRLMFNEVLPNCTAPLIV
                     QASLGISSAILDAAALGFLGLGAQPPSPEWGTMLADAREFVLRAWWVVTFPGLAILIT
                     VLAFNLLGDGLRDAFDPKLKR"
     gene            complement(4301..5305)
                     /locus_tag="PLB25_01690"
     CDS             complement(4301..5305)
                     /locus_tag="PLB25_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE40339.1"
                     /translation="MLRFLLTRFSLIIPTFVGMTLVAFFLIRMVPGDPIETLAGERGI
                     DAARHAELLKVYGLDRPVLVQYGIYIGRVLQGDLGKSIITHDPVLREFLALFPATIEL
                     ALCAILFALLIGVPAGILAAVKRNTILDHGVMGVSLTGYSMPIFWWGLLLILLFSVQL
                     DLTPVSGRISVQYFIEPVTGFLLIDTWLAGDRDAFWSAATHLILPTIVLGTNPLAVIA
                     RMTRSAMLEVLGEDYIRTARAKGLSNFRVVALHALRNALIPVVTVIGLQVGVLFTGAI
                     LTETIFSWPGVGKWMIEAIGRRDYPVLQGGMLLLGGMVMLVNLLVDVTYGIINPRIRH
                     "
     gene            complement(5403..5837)
                     /locus_tag="PLB25_01695"
     CDS             complement(5403..5837)
                     /locus_tag="PLB25_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HOE40340.1"
                     /translation="MATEKKPPAKKAVSVTKVAPAPKASAKKAASAKAAAPAKKVTAK
                     AAAPAKKAASEKKAPAAKRSVNAAFMKALTPSGELAAVVGAKALPRTEVVKKLWDYIK
                     KNELQDAVNKRLVNADAKLKEVFGKAQVTMFEIAGLIGKHLK"
     gene            6026..6547
                     /gene="rraA"
                     /locus_tag="PLB25_01700"
     CDS             6026..6547
                     /gene="rraA"
                     /locus_tag="PLB25_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HOE40341.1"
                     /translation="MSKPFATCDLCDAHKNDSPSAFRVLPPVFRDFGAQRVFSGPVTT
                     VKCFEDNSLVKAAVDSCGFIDTPEGRVGKVLVVAGAGSLQRALLGGNLGTAAAKNGWA
                     GVVIDGCVRDVAELAGQNVGIRALACMPMPTEKKNQGLKDLPVQIQGVWVNPGDWLYA
                     DADGMVVSDKSLV"
     gene            complement(6795..6983)
                     /locus_tag="PLB25_01705"
     CDS             complement(6795..6983)
                     /locus_tag="PLB25_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40342.1"
                     /translation="MRTAGITLRALHSTATPNRRLAERGRLHTLHGAGLVLCLAGVVL
                     AGQKLLTDLRTVAEKPVD"
     gene            complement(7702..8646)
                     /locus_tag="PLB25_01710"
     CDS             complement(7702..8646)
                     /locus_tag="PLB25_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOE40343.1"
                     /translation="MKPTDTTPSLPPRWLAYGLLALSMALVGSYVALSKPLAAALPVF
                     LLAWMRFGIGALAMPHWLIKPSNEPVMTGKTRWLIFLESFLGNFLFTICMLLGVSMTT
                     AVSAGVIMAAIPAVVALLSWAFLRERIGLRVWAAIALAAFGIGLFSLSKSELSAHTGQ
                     GLDANLMPESNWVGNLLVFGAVVCEASYAVIGKKLTGSVSPKRITALINLWGFALMTP
                     MGLAAAWSFDFGTVTPGTWLLLVFYALAASVWTVWLWMTGLKTVPAGQAGVFTVMLPI
                     AAAAVGVLALGEALTGLQVLAFGLSLGGVVLATLPGRK"
     gene            8940..13202
                     /locus_tag="PLB25_01715"
     CDS             8940..13202
                     /locus_tag="PLB25_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE40344.1"
                     /translation="MKVRLIPMLLRLQGWRLSVIVAFGTVVAVELIVSVMGLILKGEV
                     TPDYLLTGLVAAGIVAPLSLMLLSYLLQELARQQQQGLSRSVESAQSRLQVALDSTDE
                     GILMVADNGKVLSTNKRFIELWRVPQTLAETGQDAPLLAHVLDQLVDPAAFIDQVKRL
                     YQSHEEARDTLRFKDGRVFARYTRALSFGSEHGRIWCFKDITEQSLAQAALAEREAIY
                     RTILDNVDASIYLKDIKGRYLFANRSVRELWQVEMDAIVGFGDEKFFDAATTDQIRLN
                     DRRVLEGGETLRAEEVNTVPATGTTTVFQSTKLPLRYADGSVYALCGISMDMTARKQA
                     ELALAEREAQLRTLIEAVPDSIQFKDGDGRWLVANSVCLRLFGLDGLAWQNRSDSEIG
                     TLYPHLATEMDSCQSSDEAAWTSGEAIFRTEECRVERQGKAIHFDMIKVPLFDAQHKR
                     RALVIVGRDISERKRAERNLNMAVEVAQLVLWELDFVNQRLLYDRSFLSRLGLNAEQA
                     PGSLPEWTERVHPDDLASFRAQVEQALKPEGSVFDLEYRMSGKAGQYHWIHSRARIVQ
                     RGTDGQALLAVGTSMNITARKQVEEAVRISEEQSRNLASMLRLMCDNVPDMIWAKDLE
                     KRYIFANQAVCAQLLNARDTDEPLGQTDLFFAQRERAARPDHPQWHTFGELCQDSDTI
                     TLERGGPSVFEEFGNVKGKMLFLEVHKAPFLDKNGSVIGTVGSARDITVRKREQDQLL
                     LSASVFTHAREGIMITDAAGTIIEVNAAFTYITGYQRHEVLGQNPRMLSSGRQNNDFY
                     AALWRDLNEKGHWYGEIWNRRKNGQIYPEMQTISVVRDAGGNVLQYVALFSDITAIKE
                     HERQLEHIAHYDVLTTLPNRVLFADRLHQAMVQAQRHGQLLAVAYLDLDGFKAINDRH
                     GHDAGDQLLIALSARMKESLREGDTLSRLGGDEFVAVLLELADVSASMQLLVRLLNAA
                     AQPVQVGDFLLQVSASVGVTFYPQAAEVDADQLLRQSDQAMYQAKLAGKNRYHIFDAE
                     QDSNMRGHHESLERIRRALTEQEFVLYYQPKVNMRSGRVIGAEALIRWQHPEKGLLLP
                     AQFLPLIEDHPLAVDIGEWVIASALKQMAVWREVGLNLPVSVNVGARQLQQANFVERL
                     RALLAAHPAHRPGDLELEVLETSALEDLARVSQIIEFCREFGVKFALDDFGTGYSSLT
                     YLKRLPVTQLKIDQSFVRDMLDDPDDLAILEGVIGLSRAFNREVIAEGVETVAHGEML
                     LQLGCELAQGFGIARPMPGADMPAWAAAWQPDPVWFDRPSVSRDDLPLLFAGVEHRAW
                     IVALEAFIKGERAAAPPDGQLCRFGQWLDGERQVRHGGPVAFQAIKRVHRQVHLLAAK
                     TLLLHSSGCDQDALDCLPKLHALRDAMLVQLNALLHEGWQQR"
     gene            13794..14051
                     /locus_tag="PLB25_01720"
     CDS             13794..14051
                     /locus_tag="PLB25_01720"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HOE40345.1"
                     /translation="MKSTQALNPEKHISSEVGEESVEMPEPDCAKAIDIGPVRDELIR
                     QTAYSNYEARNHEEGHELLDWLAAESQVNQMPGKVSPSINS"
     gene            14209..14418
                     /locus_tag="PLB25_01725"
     CDS             14209..14418
                     /locus_tag="PLB25_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40346.1"
                     /translation="MPTGPLLRVARQDHHPRWRERLTDPTRLAEDATPMLTMVHRQRV
                     IAGKAAYAMRKQPVKPVPGINRSAM"
     gene            14723..>15450
                     /locus_tag="PLB25_01730"
     CDS             14723..>15450
                     /locus_tag="PLB25_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE40347.1"
                     /translation="MFGLMQHQPLLISSLIEFAERHHGEGEVVSRRVEGDIHRYTYKD
                     LALRSRKLAQALDAQGLAPGERVASLAWNGYRHLELYYGVSGSARVLHTLNPRLHPEQ
                     IAWIINHATDAALCFDLSFLPIVKAIHGYCPSVKLYVALCDADKLPVDSGIPNLVSYE
                     DWIQFRSGDYAWPQFDENSASSLCYTSGTTGNPKGALYSHRSTVLHALAGSLPDALNM
                     SARESVLSVVPMFHVNAWGLPYAGA"
BASE COUNT         3195 a         4506 c         4677 g         3072 t
ORIGIN      
        1 tcaggaagtt gtccgggtcg ccgttgtcac cggtccagcc cagcatgccc atctgatgct
       61 caccggcttg catgcgtttg cggtattcgc cccactcaaa actcttgatt tcggccttga
      121 tgccgacctt ggccaggtca gcctgcatca gctcggcaat gcgtttggcg ttggggttgt
      181 aggggcgctg taccggcatg gcccacaggt cggtgctaaa gccgttgggc aggccagcct
      241 cggccagcag cttcttggcg gctgccgggt caaacgcgtc gtccttgatg gccttgttgt
      301 aagaccactg ggtcggcgga atcgggttgg tggcaggcac gccggtgctc aggtacaccg
      361 ccgccacaat ggccttcttg tcgatggcca tgttgacagc cttgcgcacg cgcacgtcgt
      421 caaacggctt cttggtggtg ttgtaggcca ggtagccgat gttcaggccg ggttgctcca
      481 gcacggtgac gttggggtcc ttgcggatcg catccagatc agctgggttg gggtagggca
      541 tgacgtggca ttcacccttt tgcagcttgg cccagcgcac cgaggcatcc ggcgtaatgg
      601 agaagatcag gtcgtcaatc ttggccttgc cagcccagta cacggggttg gccttgtaac
      661 ggatgatggc atccttctga tactgcacca gcatgaaggg gccggtaccg acaggttcct
      721 ggtcaatttt ttccggcgtg ccagccttga gcatggcaat cgcgtattcc ttggactgaa
      781 ccccggcgta ttgcatggcc aggttggaca ggaacggcgc ttcgggtgca ttcagcacaa
      841 tcctgacggt gtagtcatcg accttgtcaa ccgacttgag cagcttgggc atgcccatgt
      901 cgccaaagta ggcgtggttg gaactggtga ccttgaagta ggggtcgttt tctttccact
      961 gacgctcgaa catgaacaaa atgtcgtcgg cgttgaagtc gcgcgtcggc ttgaagtttt
     1021 tgttggactg ccatttcacg cctttgcgca gcttgaagac atattccagg ccgtcttttg
     1081 agacggtcca gctttcagcc agacctggaa ccaccttggt gccgccacgc tcgaaatcca
     1141 ccaggttgtc gtaaatctgc tcgctggcat caaacgatgt accggtggtg ttgatacttg
     1201 gggcaaagtt ttcggggctg ccttctgagc agtaaaccag cgtttttgcc gacaccggtg
     1261 aaagcgccag caatgcaggc aaagccagca cacacagcgc ggttcgctta tgtgcgaact
     1321 tatggggggt cgtcgttttg gaaatcgtca ttttgtagac tcctgacagg ttaatgctgc
     1381 gctgcagcgg gttggacaaa ttgttccgcc agatggcagg caagctggcg tccggcgagt
     1441 tcgcgtaaaa ctggtcgctc tgactggcac tgcggtgtca catgcgggca gcgggttgaa
     1501 aacacgcagc cgctgggcgg gttcaggggc gacggcaact cacccttgag tacgatgcgc
     1561 tgcctggaaa cccctgagcc ggcaatgccc ggcgtggatg ccagcaacgc ctgggtgtag
     1621 gggtgcagcg gctgcgcaaa cagggttttc ttgtcgccat gttcgaccgc gtggccaagg
     1681 tacatcacca tcacgtcatg ggcaatgtgg cgcaccacac ccaggtcgtg cgagataaac
     1741 aggtaggcca ggccaagctc atgctgcaag tcggccagca aattgagcac ctgcgcctgg
     1801 attgacacgt ccagcgccga caccggctca tcggccacga tcaggccggg cttgagcatc
     1861 agggcacggg caatcgcgat gcgctggcgc tggccacccg agaacatgtg cgggtagcgg
     1921 tcgaaatgct cggggcgcaa gcccaccagg gccagcatgg cacgggcctg ctcggcgcgt
     1981 tcatcagcac cgaggtcggt gttgatttcc agcggtgctt gcagaattgc accaattttc
     2041 ttgcgcgggt tcagcgagcc aaacgggttc tgaaacacca gttgcacggt gcgccgcagt
     2101 gcctggcgct gttgcgtgct ggccccaacc acatcaacct tgcccagcgc caattgaccc
     2161 gaggtggggg cctcgatcag ggccaccatg cgcgccaagg ttgatttgcc gcagcctgac
     2221 tcgcctacca ccgccagcgt cttgccgggg ttgatcgaaa atgacacgcc gctgacggct
     2281 tgcaaatggt cggcttttct gaaggcccca tggctgatct tgtaaacctg cttgaggtcg
     2341 cgggccagca ccaccggctc caccgatggg tcggatggct tgtgcggctg gctcatgcgg
     2401 gggctcctga tgcggccacg ccggactcaa gcgggtagtg gcagcgcacc atgccgcttt
     2461 gccattcgcg caaggccggg cgctggtctt tgcagtgttg ggtgacgtaa ctgcagcgcg
     2521 gcgcaaacag gcagccgccg ggccggtcat agaggccggg caccatgccc ggtatggtgg
     2581 ccaggcgggt caacccgtcg ctgcgctcgg gcatggcggc catcaaggcc tcggtataag
     2641 gatgctgggg cgcctcaaaa atatcgtggg cgctgcgttc ttccatgatt tgcccggcat
     2701 acatcaccgc cacgcgctgc gccatttcgg acaccacgcc catgttgtgg gtgatcagca
     2761 ccagcgccat gttgcgctct ttctgcaggt tgcgcagcag gtccagaatc tgtgcctgaa
     2821 tcgtcacgtc gagcgccgtg gtgggttcgt cggctatcag caacttgggg ttgcaggcaa
     2881 tcgccatggc aatcatgaca cgctggctca tgccgccaga gagttggtgc gggtagatgc
     2941 ccagacggct ctcgggggcc ggaatgccca cctgctccag caattcgatt gagcgcttgc
     3001 gcgccgctgc cttgtccagc cccaggtgca actggagcgt ctcggccatc tgaaagccga
     3061 ccgtgaaaca ggggttcagg ctggtggtcg ggtcctggaa aatcatcgcc aggtctttgc
     3121 cggtcagctt gcggcgctca cgctcggaga gtttgagcag atcgtggccg tcaaagtgca
     3181 gcttgtcggc gctgatgcgc ccagggtagg ccaccaggcc catcagcgcc atcatggtga
     3241 cgcttttgcc cgagcccgat tcgcccacga tgcccagcac gtcaccggct tgcagcgtca
     3301 tggaaacccc gtcaaccgcg tgcatgatcg cagttttgga cgggaactgg accgacaggt
     3361 tttcgatttc aagcagggcc atgggcattc tttcagcgct tgagcttggg gtcaaacgcg
     3421 tcgcgcaggc catcaccgag caggttgaag gccagcacgg tgatcaaaat cgccaggccg
     3481 gggaatgtca ccacccacca ggcgcgcaac acaaattcgc gcgcatcggc cagcatggtg
     3541 ccccactcgg gcgagggtgg ctgggcaccc agacctagaa agcccagtgc tgcagcatcc
     3601 agaatggccg acgaaatgcc cagtgaggcc tgcacgatca agggtgccgt gcagttgggc
     3661 aacacttcat tgaacatcag gcgcaggtgg ccggcaccat tcatgcgtgc ggccgtcaca
     3721 tagtcgcgcg acatctcgga aataaccgct gcacgggtga tgcgaacgta atgtggcaac
     3781 acaacaatcg ccactgccag catggcattc atcagtccag ggcccaaaat ggccacaatc
     3841 acaatcgcca gcagcaggct cggcagggtc aggatgatgt ccatcaagcg catcaccgcc
     3901 acttcataaa tgcccctgaa atagcctgcg gtcagaccca gcacggtgcc cgcgatgacc
     3961 gacaacgcca ccaccgccag gccaatcgcc agcgacaggc gcgcgccgtg aatcagccgc
     4021 gagagaatgt cgcggccaat cgcgtcggtg cccagccaat gcgcgcttga gccaccggct
     4081 tgccaggcag gcggggttaa aaagacgctt gagtcggtgg catccggcgg gtaaggcgca
     4141 atcagcgggg caaaggccgc catcagcagc accgcaagca caatcagcaa gccagccaca
     4201 gcacctttgt tggtttgaaa atagtcccag aattcgcgca acgggtgctg cggcgcttgg
     4261 gcaggggatt cggtcaaggt ggtcaagatg caagcctgat ttagtgccgg atgcgcgggt
     4321 tgatgatgcc gtaggtcacg tccaccagca gattgaccag catcaccatg ccgcccagca
     4381 gcagcatgcc gccttgcagc accgggtagt cacgtcggcc aatcgcttcg atcatccatt
     4441 tgccaacgcc cggccatgaa aaaatggtct cggtcaggat ggcaccggtg aacagcacgc
     4501 ctacctgcag gccaatcact gtcaccaccg ggatcagggc attgcgcagc gcatgcagcg
     4561 cgacgacccg aaagttggac agacccttgg cacgcgcagt gcggatgtag tcttcgccca
     4621 gcacttcaag catggctgag cgggtcatgc gggcgatcac cgccagcggg ttggtgccca
     4681 gcacaatggt cggcaaaatc aaatgggtgg cggctgacca gaaagcgtcc cggtcgcccg
     4741 ccagccaggt gtcgatcagc aaaaagccgg tcaccggttc gatgaaatat tgcaccgaaa
     4801 tgcgcccgga caccggtgtc aggtcgagtt gcaccgaaaa cagcaggatc agcagcaatc
     4861 cccaccagaa gatcggcatt gaatagccgg tcagcgacac ccccatgaca ccgtggtcca
     4921 gaatcgtgtt gcgtttgaca gccgccagaa tgccggccgg cacgccaatc agcagcgcaa
     4981 acaaaatggc acacagggcc agctcgatgg tggccggaaa caaggccaga aattccctga
     5041 gcacgggatc atgggtgatg atggacttgc ccagatcgcc ctgcagcacg cgaccaatgt
     5101 agatgccata ctgcaccagc accgggcgat caaggccata caccttgagc agctcggcat
     5161 ggcgtgcggc atcaatgccg cgctcacccg ccaacgtttc gatcggatcg ccaggcacca
     5221 tccggatcag aaaaaacgcc accagcgtca tgccgacaaa cgtcggaatg atcaggctga
     5281 agcgggtcag aaggaaacgc agcattggcg gagttcctag ggttttttga atgggcgcaa
     5341 tgatgccaca aagcaaacag ccgacatgcg tcggctgttt gcttggccgc agatctgaac
     5401 agttacttca gatgtttgcc aatcaggccg gcaatctcaa acatggtgac ctgcgccttg
     5461 ccaaaaacct ccttgagctt ggcatctgca ttgaccaggc gcttgttgac cgcatcttgc
     5521 aattcgttct ttttgatgta gtcccacagc ttcttgacga cttcggtgcg cggtagcgct
     5581 ttggcgccaa ccaccgcagc aagttcgcca ctgggagtca gggctttcat gaaagccgcg
     5641 ttgacgctgc gtttggctgc aggtgctttt ttctcggatg ctgccttttt ggccggggca
     5701 gcggctttgg cggttacctt tttggctgga gcagcggctt tggcagaagc agcctttttg
     5761 gccgatgctt tgggtgcagg tgctaccttg gtgacagaaa cagctttttt agctggtggt
     5821 tttttttcag ttgccatgtg tgttgtccta tgaatagttg atcaatcacc agcgatatca
     5881 gccaatttgc tgggtgagac gaatgctaat ggagaaacct gggctttcca acccaggaga
     5941 tgcctttttc attgggcaat agcatctttt tgtgaccgtc tggtgtaccg cctacaattt
     6001 ttcaactttc acaaggagat tcaagatgag caagcctttt gccacctgtg atttgtgtga
     6061 tgcgcacaaa aacgattcac ccagcgcctt tcgggtgttg ccgccggttt ttcgcgattt
     6121 tggcgcgcaa cgggtttttt ccgggccggt gaccacggtc aagtgttttg aagacaactc
     6181 attggtcaag gcggcggtgg actcgtgcgg ctttatcgac acgccagagg gccgggttgg
     6241 caaggtgctg gtggttgccg gtgccggttc gctgcagcgc gcgctgcttg gtggcaacct
     6301 gggcactgct gccgccaaaa acggctgggc cggcgtggtg attgacggat gtgtgcgcga
     6361 cgtcgcggag ctggccgggc agaacgtggg cattcgcgcc ctggcctgca tgccgatgcc
     6421 gaccgagaaa aaaaaccagg ggctcaaaga tctgccggtg cagattcagg gtgtctgggt
     6481 caatccgggc gactggctgt atgccgacgc ggatggcatg gtggtgagtg acaagtcgct
     6541 ggtttagcgc aaggctggca gacgccgcgt ctccacctct gcggcaagtc ggcatggcag
     6601 gtggtcatgg gcggccttct ggcatgctcg cgcaggcaca gagtccaggc tttttcaacc
     6661 tgttgcatgc gttgctggca tgccgccgac atgccagaga cgggtactgc cggggtcaat
     6721 tatgaagctg cgattctaaa gaaaatcagc ctgcagcccc cgtccttcat gcgcgagcag
     6781 ctatattttc aggactaatc cacgggcttt tcggcaactg tccgcaggtc cgtcagcagc
     6841 ttttgccccg ccagcaccac acccgccaga cacagcacca gaccagcccc gtgcagggtg
     6901 tgcagtctgc cgcgctcagc caagcggcgg tttggtgttg cagtcgaatg caatgcacgc
     6961 agggtaatcc ccgcggtgcg catgcaagtc aaaacgcagc gatgccgcag aactgattga
     7021 cggcatggac ttgatcctgg ttggctcagt tggacgtgct tgagcaggcc gctggcagcg
     7081 cgtctaggtc aagcgaaagc cgcagctcca atccgtgcgg gtttgggcgg tttgagcagg
     7141 tcagccttgt ggcatggctg cgcccagcat gaggcagcaa gctgcggggg gagatgacga
     7201 cgctctctac tgcccgcatg tcgtcgtagc ggacgaggga gaagcttgcg ccccagtcgc
     7261 gctggcagtg gttcaatcga atgcgtagcg ctgtcaccca atgggtgaac cccatcgaag
     7321 ctaaaaaggt aaatgcacat aaggacttag agatgacaac aagcggtcaa ctgaggcttc
     7381 aagtctctga caactcaagt ctttgcaatt ttcgagaaga attcaagaaa gtcatgaagt
     7441 cattcgtcgc aagggcgcaa cccccacaca ttcaaatagg caccgactgc ctgagtagga
     7501 gtcgcgccct cgcgacgaag gctgttcaat tccggcttgt ctggcttagg tcgatggttc
     7561 aacagggcag cgtcagagtg cctcggttgg acggcgctgt tcacctcaaa gggtttgttc
     7621 aaggtgcatc tagcactgtt ctggcgggca ttcattgcac aaacggcaga ctggtctgaa
     7681 aaaggggcaa tcagcgcggc actacttgcg tcccggcagc gttgccaata caaccccgcc
     7741 caagctcagc ccaaacgcca gcacctgcag gccggttagc gcctcgccca gcgccagcac
     7801 accgaccgca gcggctgcta tcggcagcat cacggtgaac accccggctt gcccggcggg
     7861 cacggttttc aggccggtca tccacagcca gacggtccag acgctggcgg ccagcgcata
     7921 aaacaccagc agcagccagg tgccaggtgt gaccgtgcca aagtcaaatg accaggccgc
     7981 ggccaacccc atcggggtca tcagtgcaaa accccacaag ttgatcagcg cagtgatgcg
     8041 cttgggcgac acgctgccgg tgagcttctt gccaatcacc gcgtaggacg cttcgcacac
     8101 cacagcgcca aacaccagca ggttgccgac ccagttgctt tcaggcatca aattggcatc
     8161 cagcccctgt ccagtatgcg cagacagctc tgattttgat agtgaaaaca agccaatccc
     8221 gaacgccgcc agggcgatgg cagcccacac ccgcaggccg atgcgctcgc gcagaaaggc
     8281 ccagctcagc agtgccacca cggccggaat ggccgccatg atgaccccgg ccgacacggc
     8341 ggtggtcatg ctgacaccca gcaacatgca aatggtgaac aagaagttgc ccaaaaacga
     8401 ctccagaaag atcagccagc gtgtcttgcc ggtcatcacc ggttcgttgg acggcttgat
     8461 cagccagtgc ggcatggcca gcgcaccaat gccaaatcgc atccaggcca gcaaaaaaac
     8521 cggcagtgcc gctgccagcg gtttggacag ggccacataa ctgccgacca acgccatgct
     8581 cagggccaat agcccatagg ccagccagcg cggcggcaaa ctcggggtgg tgtcggttgg
     8641 tttcattggc taatatgggg gctggcaagc agggctgcat catgcctgaa gcgaggcgcg
     8701 cacccctgct ggcgcaatgg cgcaaaaatc gggccatcgc cgacccgcca agctattctt
     8761 acaattgatc aaacgtcgtt caggagcaat ttcatcacca aaccattcgc gttcgggcat
     8821 ctagccgcag gcctggcaga cggatgtccc tttgcaacga tggttgcgat gggcgcgagt
     8881 gggcaggcaa cagcctcgtg gacgattcgc aaacgcaatt gccccggagc aagccaacca
     8941 tgaaagtccg gctgatcccg atgctgctgc gcttgcaagg ctggcgtttg tcggtgatcg
     9001 tcgcattcgg aacagtggtc gcggtggagt tgatcgtgtc cgtcatgggc ctgatcctaa
     9061 agggcgaggt cacacctgac tatttgctga ccggtctcgt cgccgctggc atcgtggcgc
     9121 cactcagtct gatgttgttg agctatttgt tgcaagaact tgcccggcaa caacaacaag
     9181 gtttgtcccg cagtgtggaa agtgcgcaga gcaggttgca agtggcgctg gactcgaccg
     9241 acgaaggcat cctgatggtt gccgacaatg gcaaagtgct gtccaccaac aagcgcttta
     9301 tcgaactctg gcgcgtgccg cagacgcttg ccgagaccgg ccaggatgcg ccgctgctgg
     9361 cacacgtact tgatcaattg gtggaccctg cagctttcat tgaccaggta aaacgccttt
     9421 accagagcca tgaagaagcg cgcgacaccc tgcgcttcaa ggatggtcgg gtgtttgccc
     9481 ggtacacccg ggccttgtct tttggcagcg agcatggccg catctggtgc ttcaaggaca
     9541 tcaccgaaca gtcgctggcc caggcggcac tggccgagcg agaggcaatt taccggacca
     9601 ttctcgacaa cgtcgatgcc agtatctatc tcaaggacat caaggggcgc tatctgtttg
     9661 ccaaccgatc tgtgcgtgaa ctctggcaag tcgaaatgga tgccattgtc ggattcggtg
     9721 atgagaagtt tttcgacgca gccactaccg accaaatccg gctcaatgat cgccgcgtgc
     9781 tggagggcgg tgaaaccctg agggctgaag aggtcaatac ggtgcccgcc accggcacca
     9841 ccaccgtttt tcagtcaacc aaattgccac tgcgttatgc cgacggcagt gtttacgccc
     9901 tgtgtggcat ttcaatggac atgaccgcgc gcaagcaggc agagctggcc ttggccgagc
     9961 gcgaggccca actgcggacc ctgatcgagg cggttcccga ctccatccag ttcaaggacg
    10021 gcgacggacg ctggttggtg gccaacagcg tgtgcctgcg tcttttcggt ctggatgggc
    10081 tggcctggca gaaccgctcc gacagcgaaa ttggcaccct gtacccacac ctggccaccg
    10141 aaatggactc ctgccaaagc agtgatgaag cggcctggac atctggcgag gctatctttc
    10201 gcaccgaaga atgcagggtc gaacgccaag gcaaggcgat tcatttcgac atgatcaagg
    10261 tgccactgtt cgatgcgcag cacaagcgcc gcgccttggt cattgtgggc cgcgacatca
    10321 gcgagcgaaa gcgcgccgag cgcaatctca acatggcggt tgaagttgcc caactggtgc
    10381 tttgggagct tgattttgtc aaccaacgac ttctgtatga ccggtcattt ttgtccagac
    10441 ttggcctgaa cgccgagcag gcaccgggca gtttgcccga atggaccgag cgcgtgcatc
    10501 cggacgacct tgcgtcattt cgggctcagg tcgaacaagc cttaaagcct gaaggttcgg
    10561 tattcgatct ggagtatcga atgtcaggca aggcaggcca ataccattgg atccacagcc
    10621 gcgcccggat tgtccagcgt ggcaccgacg ggcaagccct gctcgcggtc ggcacctcga
    10681 tgaacatcac ggcgcgcaag caggttgagg aagctgtgcg catcagcgaa gagcagtcgc
    10741 gcaacctggc ctcaatgctg cgcctgatgt gcgacaacgt gccggacatg atctgggcca
    10801 aggacctgga gaagcgctat atctttgcca accaggcggt ttgcgcccag ttgctcaatg
    10861 cccgcgatac cgacgagccg ctgggccaga ccgatctctt ttttgcccag cgcgaacgtg
    10921 ccgctcgtcc cgatcatccg cagtggcata cctttggtga gctttgccag gactcggaca
    10981 cgatcaccct tgagcggggc gggccgtcgg tcttcgaaga gttcggcaac gtcaaaggca
    11041 agatgctttt ccttgaggtg cacaaggcac cgttcctcga caagaacggc agcgtcatcg
    11101 gcacggtagg ctcggcgcgc gacatcaccg tgcgcaagcg cgagcaagac cagctccttc
    11161 tgtcggcgag cgttttcacc catgcccgcg aaggcatcat gatcaccgat gcagcgggca
    11221 cgatcattga ggtcaacgcg gcgtttacct acatcaccgg ctaccagcgg cacgaagtcc
    11281 ttggccagaa cccgcgcatg ctgagttctg ggcgccagaa caatgacttc tatgccgccc
    11341 tgtggcgcga tctgaacgaa aaaggccact ggtacggtga aatctggaac cgccgcaaaa
    11401 acggccagat ctatcccgag atgcagacca tcagcgtggt gcgcgatgcc gggggcaatg
    11461 tcctgcagta tgtggcgttg ttctccgaca tcacggcgat caaggaacac gagcgccagc
    11521 tcgaacacat cgcccactat gacgtgctga ccacgctgcc caaccgcgtg ctgtttgccg
    11581 atcgcttgca tcaggccatg gtgcaggcgc agcggcacgg gcaattgctg gcggtcgcct
    11641 atcttgatct cgatggcttc aaggccatca acgaccggca tggccacgat gccggcgacc
    11701 agttgctgat cgcgctgtcg gcccgcatga aggagtcgct gcgcgaaggc gacacattgt
    11761 ctcgcctggg cggcgacgag tttgtcgccg tgctgcttga gctggccgat gtatctgcca
    11821 gcatgcagtt gcttgtccgc ctgctcaatg ccgcagccca gccggtgcag gtaggcgatt
    11881 ttctactgca ggtgtcagcc agtgtgggtg tcaccttcta cccgcaggcg gcagaggtgg
    11941 atgccgatca actgctgcgc cagtccgatc aggcgatgta ccaggccaag ctggctggca
    12001 agaaccgcta tcacatcttt gatgccgagc aggacagtaa catgcgcggc catcacgaaa
    12061 gcctggaacg tatccgccgc gccttgactg agcaagaatt tgtgctctat taccagccca
    12121 aagtcaacat gcgcagcggc cgggtgatcg gggccgaggc actgatccgt tggcaacacc
    12181 cggaaaaagg tctgttgcta ccggcccagt ttctgccatt gatcgaggac catccgctgg
    12241 cggtcgacat cggtgaatgg gtgatcgcca gcgccctgaa gcagatggca gtttggcgcg
    12301 aggtcggttt gaacctcccg gtcagcgtca atgtcggggc ccgccagttg cagcaggcca
    12361 actttgttga gcgcctgcgt gcgttgttgg cggcacatcc ggcgcaccgg ccgggcgatc
    12421 tcgaactcga agtgctggaa accagcgccc tcgaagacct ggcccgagtt tcccagatca
    12481 ttgagttctg ccgggaattt ggcgtgaaat ttgcccttga tgactttggc accggctatt
    12541 cctcgctgac ttacctcaag cgcctgccgg ttacccagct caagatcgac cagagtttcg
    12601 tgcgcgacat gctcgacgac ccggatgatc tggccattct cgaaggcgtg atcggcttgt
    12661 ccagagcctt caaccgcgag gtcatcgccg agggtgtcga gactgtggca cacggcgaga
    12721 tgctgttgca actgggctgc gaactggctc agggctttgg catcgcacgg cccatgccag
    12781 gtgccgatat gcccgcctgg gctgctgcgt ggcagcccga tccggtctgg tttgatcggc
    12841 cttccgtcag tcgggacgat ttgccgctgc tctttgccgg tgttgagcat cgtgcctgga
    12901 tcgtggcgct ggaggcattt atcaagggtg agcgtgcagc ggcaccgcct gatggtcagc
    12961 tttgccgctt cggtcaatgg ctggacggcg agcgccaggt gcgccatggt gggccagtcg
    13021 ccttccaggc catcaaacgc gtgcaccggc aggtgcattt gctggcggca aagacgctgc
    13081 tactgcattc cagcggctgc gatcaggatg ctttggactg tctgccgaaa ctccatgcgt
    13141 tgcgagatgc catgctggtg cagttgaatg cactgttgca tgagggctgg cagcagcgct
    13201 aaggatgtgg tcgggaataa ctccccgttt tgcaccaagg agttgggatg cacggctagg
    13261 cgcaagacgc gccgtttagc tggctaaaca agcgtcttgc aacgacgctg tgcgcccaaa
    13321 tccgccggtg caaatagggg aagttatttc tgaccacatc ctaaggcggc acgacatggt
    13381 gtcagcatgg cacggctgtt tgatgcggct tcagtagcgg ggtgcaaatc aggcattttc
    13441 atggcacggt gatttgtcgc tggcgggtct gcttgagtgg cagggaattg aatcacaaca
    13501 ttgataaacc ggtgcctggc agggctcaat gcgatggcga tgaaattggc ttttggtgaa
    13561 attggtcgca aaccaaggcg acatgggagt tagcgcctga aggacttcgg catcgaccgg
    13621 tgaggtcctt tgcttcgtcg cctggcaaaa aattgcaaag gtattcccaa aacacatcgc
    13681 ctttgtgtag ccgcgcacag accgccagtg tgcgacgcaa ctacattcaa ctctgactca
    13741 gtcgtgtgct cataacgcgc atattgctcc aacccaacaa aggtgaaaca ctcatgaaaa
    13801 gtacccaggc attgaacccc gaaaagcaca tcagcagcga ggtcggcgag gaatctgtag
    13861 aaatgccaga accagattgc gccaaagcca ttgacattgg ccccgtgcga gatgaattga
    13921 ttcgtcaaac tgcctattcc aactacgagg cgcgaaacca cgaggagggt cacgagttgc
    13981 tggactggct ggcggcagaa tcgcaggtga accagatgcc aggcaaggtt agcccgtcca
    14041 tcaattcata ggcgagctgg cagcagcaaa cattgctgcc gaccgaagtt gtcgaacaag
    14101 ccgagatttg gttcaggctg cggtgccctg tacatcgctt cggatgctgc gctggctggg
    14161 tatcagtcat cgcggctgca gcgacaacaa tgtagcgcct gtgtggctat gccaactggt
    14221 cccttgctgc gagtggcccg ccaagaccat cacccgcgct ggcgggagcg cctgaccgac
    14281 ccaacccgat tggctgagga tgccacaccg atgctgacca tggtgcatcg acagcgcgtg
    14341 atagcaggca aggcggcata cgccatgcgc aaacaaccgg tgaagccggt ccctggcatc
    14401 aacaggtcgg cgatgtgatt gaacctgttc cccacgtgtg gattgcgcaa agtcacacgc
    14461 cagtggcgtc tggatcgccc ggcctggaat ctcaagcgca tggctgtacc gcgttgaaaa
    14521 gttgaacaac gcgacgaaat tccttaaaaa ttggcgtaaa tgctcggaat ttgactctga
    14581 ggcgccgacc ttctgggcag cgtctcaaca gggcaagcta ttgagatccc aagtccggca
    14641 agcagctggc gattgcgacc cgtggctggt ccagtttggc attacgatgg cagaccaatc
    14701 aacccacaaa aaggacgctg ccatgttcgg actgatgcaa catcaaccgc tgctgatttc
    14761 atcgttgatc gagtttgccg agcgccatca cggcgaaggc gaagtcgtgt cacgacgcgt
    14821 cgagggcgat attcaccgtt acacctacaa agacttggcc ttgcgctcgc gcaagttggc
    14881 gcaggcgctc gacgctcagg gcctggcgcc aggcgagcgt gttgcctcgc tggcctggaa
    14941 cggctaccgg catctggagc tttactatgg tgttagcggc tcggctcggg tgctgcacac
    15001 cctgaacccg cgcctgcacc ccgagcagat tgcctggatc atcaaccatg cgacagatgc
    15061 ggcgctgtgt tttgacctgt cgtttttgcc catcgtcaaa gccattcacg gctactgccc
    15121 gagtgtcaaa ctgtatgtgg cactgtgtga tgccgacaag ctgccggttg acagtggcat
    15181 acccaatctg gtgagttacg aggactggat tcagtttcgc agcggtgact acgcctggcc
    15241 ccaatttgac gaaaactcgg cttccagcct gtgctacacc agcggcacca ccggcaaccc
    15301 caaaggtgcg ctttacagcc accgctcgac cgtgctgcac gcgctggccg gttcgctgcc
    15361 cgatgcactc aacatgagcg cgcgcgagtc ggtgctgtcg gtggtgccga tgttccacgt
    15421 caacgcctgg ggcctgccgt atgccggggc
//
LOCUS       DAOSLL010000023        15295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4010_length_15295_cov_6.756365, whole genome shotgun sequence.
ACCESSION   DAOSLL010000023 DAOSLL010000000
VERSION     DAOSLL010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 15295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15295
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4010_length_15295_cov_6.756365"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1013)
                     /locus_tag="PLB25_01735"
                     /pseudo
     CDS             complement(84..1013)
                     /locus_tag="PLB25_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574858.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment shell
                     protein"
     gene            complement(1161..1370)
                     /locus_tag="PLB25_01740"
     CDS             complement(1161..1370)
                     /locus_tag="PLB25_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40348.1"
                     /translation="MNPIALSWLMVAFVPAAPTASFLKKLSSLLKLLAITVSGELIKD
                     QAACGCLPATLDFKSVMHAAIVYPS"
     gene            1513..2742
                     /locus_tag="PLB25_01745"
     CDS             1513..2742
                     /locus_tag="PLB25_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOE40349.1"
                     /translation="MSHSLKDPKRAWDAGMRDDDLVSIVVFEQSDSNPIDLKQVSPVH
                     FIGVKDMRQAFVAGQVAITQPKGVEEGSEIRVLWTCAVANQRSVISIADSARLGLMPV
                     SETRRQSIQLSRNKGTISLLPQAKIGDTVLACQIVAAVVPVSTQLPCPPPVTEGYFIG
                     KLASVNLSERYAAAKALRYRGYTAAKPTLESRMTDAEEDIYVQLEAAAALAAYDDPAG
                     WAFMEHKLHSPSLAVALETQLETIIVASEIPKQRSEKLLIDVLRDTQRDAELRAGAAW
                     ALGQFVSTESAVALAETFNASPLEIKMEAARALLRIAEPQIPQLIDLLKNGDPSQRDG
                     LAWVLARTGKFNPANLLAGADENLRRWVSYIVGTGKEKFDHNDVQAICQADSQVYFAA
                     SVLWQIVASWVNDLKEY"
     gene            2744..3823
                     /locus_tag="PLB25_01750"
     CDS             2744..3823
                     /locus_tag="PLB25_01750"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000970946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOE40350.1"
                     /translation="MQSQPLYRTGRGQAYVGDSLALLAELPDDSIDLVMTSPPFALRR
                     QKSYGNVAETEYVEWIRPFGQAVFRVLKPGGSFVLDLGGAYRSGIPSRSLYNFRVLLA
                     FCDDIGFHLAEDFYWFNPAKLPSPIEWVNKRKIRAKDSVNTVWWFGKTDSPKADIRKV
                     LAPYSDRMKKLIEDPASFYTPKKRPSGHDISASFGIDNGGAIPSNLLSIPNTESNSSY
                     LRLCNEFERERHPARFPVDLPAFFIKMLTDEEDVVLDIFGGSNTTGFTAEALRRKWLT
                     FELSRDYLVTSAFRFLEGHRADTVRQVLVALDNDHANYLIEESVCTLDQAKKARSKEE
                     FIGQGGLFADAVHHKTNQLLVEIPL"
     gene            complement(3931..4134)
                     /locus_tag="PLB25_01755"
                     /pseudo
     CDS             complement(3931..4134)
                     /locus_tag="PLB25_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857267.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(4131..5084)
                     /locus_tag="PLB25_01760"
     CDS             complement(4131..5084)
                     /locus_tag="PLB25_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HOE40351.1"
                     /translation="MSVVALRPAEYRAPVFDLKQVVTELAGVRQRWRESQRRSTEPGG
                     RELPSREALAQIIDSLRGVLFPMRLGPVDLRQESEDFYVGHTLDSVLHALLVQVRLEL
                     QYAVRHQPYQDALGIDTDAIKLVHDFSRELPRIRSLLDADVLAAFHGDPAARSVDEVL
                     LCYPGILAMIHHRLAHQLHVLGVPLLARIVAELAHGQTGIDIHPGATIGAGLFIDHGT
                     GVVIGETAIIGERVRIYQAVTLGAKSFPTDSSGKPIKGRPRHPIVEDDVVICAGATVL
                     GRVTIGRGASIGGNVWVTHSVPAFSHVTQAVARHDVQQSTL"
     gene            complement(5081..6079)
                     /gene="cysK"
                     /locus_tag="PLB25_01765"
     CDS             complement(5081..6079)
                     /gene="cysK"
                     /locus_tag="PLB25_01765"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015551711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOE40352.1"
                     /translation="MTHVYADNSLSIGNTPLIKLNRITDGAGATVLAKIEGRNPAYSV
                     KCRIGAALVWDAEKRGLLGAGKEIVEPTSGNTGIALAFVAAARGIPITLTMPDTMSIE
                     RRKLLLAYGAKLVLTEGAKGMKGAIAKAEEIAASDPKYVLLQQFKNPANPAIHEATTG
                     PEIWIDTDGKIDILVSGVGTGGTITGISRYIKNTQGKAILSVAVEPTASPVLTQARAG
                     EEIKPGPHKIQGIGAGFVPDVLDLSLVDAIEQVSNEDAVLFARRLAREEGILAGISCG
                     AATAVAVRLAQRPENAGKTIVVILPDSGERYLSSILFEGLFDASGLAAEPAPSVAP"
     gene            complement(6109..6576)
                     /locus_tag="PLB25_01770"
     CDS             complement(6109..6576)
                     /locus_tag="PLB25_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOE40353.1"
                     /translation="MNTANTTDSLADPLESIRSVALATGLSYAGGVPPQLAWELVSAG
                     QAVLVDVRTTEERKFVGHVPGSLHVAWASGTAMTRNPRFVRELEAKTGKDATVFLLCR
                     SGKRSDLAADAAAKAGFTRVFNVLEGFEGEIDEQRHRGDLGGWRSHGLPWVQD"
     gene            complement(6874..7335)
                     /locus_tag="PLB25_01775"
     CDS             complement(6874..7335)
                     /locus_tag="PLB25_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HOE40354.1"
                     /translation="MSLSRSLLQGASSLAAGTVFGLGLAIAQMTDPNKVLSFLDITGH
                     WDASLLFVLGAAVILSAATFHIILRKPSPRFDSRFHLPQSALIDARLVTGAAIFGMGW
                     GIAGYCPGPALASLGFGNSELFWLLAALLLGMALQAWLDRVSYPKARLDST"
     gene            complement(7332..7763)
                     /locus_tag="PLB25_01780"
     CDS             complement(7332..7763)
                     /locus_tag="PLB25_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HOE40355.1"
                     /translation="MTDFTPVSALVGGLLIGLSASLLLWLNGRIAGISGVLGGLLWPG
                     SGDRAWRGWFLAGLVLGGGGYYLVTGVTAPIRLGFPVYLLLLAGFLTGFGSARANGCT
                     SGHGVCGLARLSLRSLVATATLLIAAIATTYVVRHIVGVSS"
     gene            complement(7788..9071)
                     /locus_tag="PLB25_01785"
     CDS             complement(7788..9071)
                     /locus_tag="PLB25_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HOE40356.1"
                     /translation="MSTSARTFEFSLRAATLATALLVPLQASATNGYFSHGYGAKSQG
                     IAGVGIALPQDGLAAASNPAGTAAVGSRLDLGLTVFAPSRGADIAGNAFGPNASYSGD
                     DSKTFLIPDFGYTRQLNPSTAIGLAVFGNGGMNTDYGQNPYARFGATGSAGINLEQLF
                     ISPSIAFKVNEAHTFGAALTLAYQRFEAKGIGLFGGFSSAPANVSDQGVDGSTGVGVR
                     LGWLGKITPQLTLGATWASKISGKFDKYKGLFADAGGFDVPENYGVGLAFEATPAWTL
                     AADVQTIRYSQVGSVGNSAAGLFAGQSALGAANGPGFGWRDVNVLKLGVSHTLRPDLS
                     LRGGLSFASQPVPSSETFFNVLAPGVITRHLTLGATWTTPGGGELSGFYAYGVGETVN
                     GSGSIPPGNPPGFGGGNANVRLKENIIGISWGWKF"
     gene            9397..9729
                     /locus_tag="PLB25_01790"
                     /pseudo
     CDS             9397..9729
                     /locus_tag="PLB25_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            9741..9947
                     /locus_tag="PLB25_01795"
     CDS             9741..9947
                     /locus_tag="PLB25_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40357.1"
                     /translation="MAFSIWAGKRLAERVAGKDAGQKVFQLPIYAQPLPAPNLFGLIC
                     SPALAPFRRLGQRMLYQWYGLRDQ"
     gene            complement(9941..10774)
                     /locus_tag="PLB25_01800"
     CDS             complement(9941..10774)
                     /locus_tag="PLB25_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE40358.1"
                     /translation="MYFTVNQAATYCYTGGKAFDAAKPAIIFIHGVLNDHSVWILQTR
                     YLAHHGWNVLAVDLPGHCRSAGQPPASVEEAADFIIALMDAAGLAQAVLIGHSLGSLI
                     ALEAAARAPERVSQLVLVGVAFPMKVSHALLAASQSEPMKALEMVNVFSRSTLAPPPS
                     AMGPGTWVYGASLALGRRVLASNPQVNVFYTGFAACDSYANGETAMAQVTCPVLFVLG
                     SMDQMTPPKAAQGLIKKAHDAQVAMLPGGHQQMNETPEQMLAALNGFLKPDLCMRQPP
                     H"
     gene            complement(10887..12182)
                     /locus_tag="PLB25_01805"
     CDS             complement(10887..12182)
                     /locus_tag="PLB25_01805"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HOE40359.1"
                     /translation="MPGYSDPGFDTLALHAGAAPDPTTGARAVPIYLSTSFVFESSEH
                     AASLFNLERAGHVYSRISNPTNAVLEQRVAALEGGIGAISTASGQAALHLCIATLMGA
                     GAHIVASTALYGGSQNLLHYTLRRFGIDTTFVKPGDIDGWRAGVRPNTRLLFGETVGN
                     PGLEVLDIAAVSSIAHEAGVPLLVDSTLTSPWLIRPFDHGADLVYHSATKFLSGHGTV
                     IGGIVVDAGSFDWERSGKFPELTEPYAGFHNMVFTEESTVGAFLLRARREGLRDFGAC
                     LSPHSAWLILQGIETLSLRMTRHMENTHKVVGFLASQPFVARVGHPLLTSHPSHALAQ
                     RLLPRGAGSVFSFDLRGSREQGKKFVESLKVFSHLANVGDCRSLVIHPASTTHFRMSD
                     EALAAGGISQGTIRLSIGLEDADDLIDDLKRALKAAERA"
     gene            12602..15196
                     /locus_tag="PLB25_01810"
     CDS             12602..15196
                     /locus_tag="PLB25_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding module family 20
                     domain-containing protein"
                     /protein_id="HOE40360.1"
                     /translation="MNLIIRRLTRGSSHRLAALFLFLAGLQVPGLALEAPCPPGNASV
                     WGPAAKDFLGTSASNNSRVYFTGAEGILTEVFYPTLDRVQNVDLQLLVTDAGKTWGDE
                     ERRQKQRDISQVNRRAMLWQTVTTADNGKWKITKKIFTDPWRNALIQRVSFQTLEPGK
                     KVKDYNLYLLNNPAINNTGAGSGTSNQCSGRQGTAQGADNSRTLSANNRIMLVASEPN
                     STASALAVSVPWKTVGGQAMVSNGFVGRNDGFTDLFAGANDRTMNFRFDGAFGGNVAQ
                     MGWIDTGGNTDTSLSFDVVLAFGGSEAEAMGVANATLGTSLNALEQVYINEWLAYSQT
                     LNNQNGRADDQYYLAAMTLKSIQDKSNGAMIAGLGTPWGETSNDGNPGGYHLIWSRDL
                     FKFASALIAAGDTDSANRTVDFLFNRQMQTSSGDNPYSRPGRFPQNSFVDGTPYWNGT
                     QMDETAMPIILAWKLGRVDLWPKIKLAAEFLAHNGPATGQERWEEMGGYSPSTIAAEI
                     AGLVCAASLATAAGDPGAASFYLQKADEWRNNIANWTFTTTGFHTGCNGCNGKYYIRI
                     NANQDPNDDVNLTFGNGVGTHGERYIVDGGFLELVRMGVMSPSDWTILDTLPEYDALL
                     KQTITGKGDAWFRYNYDGYGEYNDGRNYDGSGRGRLWPIFTAERGIYEIAKASDSSKG
                     EPYRLALKAFSSPAGFIPEQVWNNSANITGWQTDTPSFYAVGSATKSMQPLSWAMGEY
                     INLIAAMNLGQSDAPTVVCQRYACDKPQSTVTFRVNATTSFGENIYLVGSSPLLSNWL
                     PASGIKLSPAQYPVWNVTLSLPAGMALEYKYVKRDAGGNAVWESGSNRSFAVPASGEL
                     VHDDIFKP"
BASE COUNT         3258 a         4474 c         4563 g         3000 t
ORIGIN      
        1 gaggccatgc tggcgatcag cgccgggtcg aacggtgcct gtgcccccgc tggtggggtt
       61 aaagcggaaa tcatgatcac cgcttacttg taggtgtgag cgtactcgtg gtacttgccg
      121 acttcaacat cgtcgagcac tgccagcgcg tcaggggtga ggaccgccag cgagcaatac
      181 aaggagatca ggtaactcgc tatcgcctgg tcgttaatgc ccatgaaccg caccgacagt
      241 cctgggcttt gctcgcctgg caaacccggc tggaacaagc caaccatgcc ctggcgcttg
      301 tcgcccacgc gcagcagcaa gatcttgctc ttgccatcgg ccaccgtgat cttgtcgctg
      361 gggatcagcg ggacaccgcg ccaggtaatg aactgcgaac caaacaggct gacagtgggc
      421 gggggcacgc cacgccgggt ggcttcgcgg ccaaaggcgg caatggccaa ggggtgggcc
      481 aggaagaacg ctggctcttt ccagaccttg gtcagcaact cgtcgagatc atcgggtgtc
      541 ggtgcaccgg taagcggaaa gatgcgctga tcgtcggtca cttgcgacaa caggccgtag
      601 tcggggttgt tgatgagttc gctttcctgg ttttccttga tggtctcgat ggtcaggcgc
      661 agttgttcct tgatctggtc atgcgggctg ctgtacaggt cagaaatgcg ggtatgcaca
      721 tccagaatcg tgctgaccgc gttgaggaaa aactcacgcg gcttttagtc gcaattgaca
      781 aatgtgagcg gcaactggtt ctcggtctgg cgtgcggtgc aggtgacctt gatcgactcc
      841 gggtctttga cggtgttgag gcgaaagatg ccagcttcca ccgccaccca ttgcagcagg
      901 tgggtcagcc agcgcggcga aacggtggac aactgcgcag tggttttggt ggcgttggcc
      961 aactggcgcg cagcgttgtc gccaagcgtg gtgtgggcaa tgtgtttggt caggaaggac
     1021 tccgtgatgg gttaaaaata attgcgtgag tgtcggcagt ccgatgccat ccatcaacat
     1081 gctatagccg ccaaacggat ggctttggcg tgttgcgcct ggtggtgctg ctcgctcagc
     1141 cggggccgat caacgcaggt tcaagatgga tagacaatag cagcgtgcat aacactcttg
     1201 aagtccaacg ttgcaggaag gcagccgcaa gctgcttgat ctttaatcaa ttcacccgat
     1261 accgtgatgg ccaataactt caaaagtgat gatagttttt tgagaaaact ggcggttgga
     1321 gccgccggca caaatgccac catcagccag cttaaagcga tgggattcaa tccgattgaa
     1381 cttgagcgtg gctctaccgg ctacaaaatc tggaaaaaaa tcaaaatcaa gcgtgtgcga
     1441 gttcctgaca ttctgtgttt gaacactggc cttcggtttg aatcgcgtgg caaaacaaaa
     1501 cctgaaattt caatgtcgca ttccctcaaa gacccaaagc gagcttggga tgccgggatg
     1561 cgtgatgatg acctggtttc aatcgttgta ttcgagcaaa gcgacagcaa tcccatcgac
     1621 ctgaaacagg tctcgccagt tcatttcatt ggtgtcaaag acatgcgtca agcgtttgtt
     1681 gccggtcagg tcgccatcac acagcccaag ggggtagaag agggatcaga aatacgcgtg
     1741 ctgtggacat gtgctgtggc caaccagcgc tctgttatca gcatcgctga ctctgccagg
     1801 cttggtctga tgcctgtcag cgaaacccgg cggcaatcga ttcagttaag ccggaacaaa
     1861 ggaacgatca gtctgctgcc acaagccaag attggtgata ccgtactggc ttgccagatt
     1921 gttgctgcag ttgtaccggt cagcacccaa ctcccatgcc cgccgccagt taccgaaggg
     1981 tatttcattg gcaaacttgc aagcgtcaac ctgagtgagc gctatgcagc cgcaaaagca
     2041 ttgcggtacc ggggttacac agcagccaag ccgacactcg aatcaaggat gacagatgcc
     2101 gaggaggaca tttacgttca actcgaagct gcggcagcgc tcgccgccta tgacgatccg
     2161 gccggctggg ccttcatgga acacaagctg cacagtccgt ccctggcagt tgcgcttgaa
     2221 acacaacttg aaacaatcat cgttgcgtcg gaaataccca agcagcgaag tgaaaaattg
     2281 ctcattgacg tgttgcggga tacacaacgt gatgccgaac tgcgcgcagg tgccgcctgg
     2341 gcgttaggtc agttcgtttc aactgaatcg gctgtcgcgc ttgccgaaac cttcaacgcc
     2401 agtccccttg agatcaagat ggaggctgca cgtgcgctgt tgcgaatcgc cgagccacag
     2461 attccccaat tgattgacct gctgaaaaac ggtgatccca gccagcgcga tggtctggcg
     2521 tgggtactag ccagaacggg caaattcaac ccggccaacc tgctggccgg agccgacgag
     2581 aacctgagac ggtgggtgag ctacatcgtc ggcactggca aagagaagtt tgatcacaac
     2641 gatgtgcaag ccatttgcca ggcagactct caggtctatt ttgctgcaag cgtgttgtgg
     2701 caaatcgtcg ccagctgggt caatgacctg aaggaatatt gacatgcaga gccaaccgct
     2761 ttaccgcacg ggccggggcc aggcctatgt gggcgattcg ctggcacttc tggccgagtt
     2821 gcctgatgac agcatcgacc tggtcatgac ctcgccgccg tttgcccttc gacgccaaaa
     2881 gtcctacggc aatgtggcag agaccgaata tgtcgagtgg atcagaccct ttggacaagc
     2941 ggtttttcgg gttctcaagc ctggtggaag ctttgttctt gacctgggtg gggcctaccg
     3001 cagcgggatt ccgtcgcggt ctttgtataa ctttcgggtt ctgctggctt tttgcgacga
     3061 catcggattt caccttgctg aagatttcta ctggttcaat ccagccaagc tgccgtcacc
     3121 cattgaatgg gtcaacaaac ggaaaattcg agccaaggat tccgtcaaca cagtttggtg
     3181 gtttggcaag accgattcgc ccaaggctga cattcgcaag gtgctggctc cttactccga
     3241 ccgtatgaag aaactgattg aagacccggc cagtttttac acgcccaaaa aacggccatc
     3301 gggccacgat atcagcgcta gctttggcat tgacaatggc ggcgcgatcc cgtccaacct
     3361 gctttccata cccaataccg agagcaattc aagttatctg cggctctgca acgaatttga
     3421 aagagagcgt catccggcgc gtttcccggt ggatcttccc gcgtttttta tcaaaatgct
     3481 caccgacgaa gaagatgtgg tgctggatat ttttggcggg tcaaacacca ccggcttcac
     3541 tgccgaagca ctccggcgaa agtggctcac ttttgagctg agccgggact atcttgtgac
     3601 atcggcattt cgttttcttg agggacaccg tgcggacacg gtcaggcaag tcttggtggc
     3661 actggacaat gaccacgcca attacctcat tgaagaatcc gtctgcactt tggatcaggc
     3721 caaaaaagcc agatcaaagg aagaattcat cggtcaaggt ggattgtttg ctgacgctgt
     3781 ccatcacaaa accaatcaac tgctggtaga aataccgctt tgatttccgt gatcgccgat
     3841 ataaaggttt ccggctttcg cgcagcagac tatagccgtc aacctgatgg ctttggcttg
     3901 ttgcgcctga tgctgctgtt cgctcaggcg cagcaactcg ccggaggcca agcccagggc
     3961 agcagccagt ttttgcaggg taaccagcga tgcagtgacc tggccgcgtt cgatttcccc
     4021 cacataagaa cggttcaggt cggcctgtcc ggcgagttgc tcttgcgacc agcctcgcgc
     4081 ttcgcgctgg cggcgcacgg tcatgccaaa gctgacgatc agcatgtcgc tcataaggtg
     4141 ctctgctgca cgtcgtggcg tgccaccgct tgggtcacat ggctaaaggc aggcacgctg
     4201 tgggtgaccc agacattgcc gccaatgctg gccccacgcc caatcgtgac ccgcccgagc
     4261 accgtggcac cggcgcaaat caccacgtcg tcttccacta tcgggtggcg cggtcgcccc
     4321 ttgatgggtt tgcctgatga atcggtcgga aagctcttgg cccccaaagt caccgcctgg
     4381 tagatgcgaa cccgctcgcc gatgatggcg gtctcgccaa tcacgacacc ggtgccgtgg
     4441 tcgatgaaca aaccggcccc gatggtggca cccggatgga tatcgatacc ggtctggcca
     4501 tgtgccagtt cagccacaat gcgcgccagc agtggcacgc ccagcacatg caactgatgc
     4561 gccagccggt gatggatcat ggccagaatg ccggggtagc acagcaacac ttcgtcgaca
     4621 ctgcgcgccg ccgggtcgcc gtggaatgcg gccagcacat cggcatcgag caagctgcga
     4681 atgcgcggca attctcgcga gaaatcgtga accagcttga tggcatcggt gtcgatgccc
     4741 aatgcatcct ggtagggctg gtgtcgaacg gcatactgca gttcgaggcg cacctgcacc
     4801 agcaaggcgt gcagcacgct gtcaagggtg tggccaacgt aaaaatcctc gctttcctgg
     4861 cgcaggtcga ctggtcccag gcgcatcgga aacagcacgc cgcgcaagct gtcgatgatt
     4921 tgcgccagcg cctcgcgcga gggcagttca cgcccgccgg gctcggtcga gcgcctttgc
     4981 gactcgcgcc agcgttgacg cacaccagcc agctctgtca caacctgttt cagatcaaac
     5041 accggtgcac ggtattcggc gggccgcagg gcaacgacgc tcatggcgca acagacggtg
     5101 ctggctctgc tgccagccct gatgcatcaa acagcccttc aaacaggatc gagctcaaat
     5161 agcgctcacc cgagtcaggc aggatcacga caatggtttt gccagcgttc tcgggccgct
     5221 gtgccagccg caccgcgacc gccgtggcgg cgccgcaaga gatgccagcc agaatgcctt
     5281 cctcgcgcgc cagccgccgg gcaaacagca ccgcatcttc gttgctgacc tgctcaatcg
     5341 catcgaccag cgacaaatcc agcacatcgg gtacaaaacc ggcgccaata ccctgaatct
     5401 tgtgcggccc gggtttgatc tcttccccag cgcgggcttg ggtgagcacc gggctggcgg
     5461 tgggttcgac tgccactgaa aggatggctt tgccctgggt gttcttgatg tagcgtgaaa
     5521 tgccggtgat ggtgccaccg gtgccgacac ccgacaccag tatgtcgatc ttgccgtcgg
     5581 tgtcaatcca gatttccggc ccggtggtgg cctcgtgaat ggccgggttg gcggggttct
     5641 tgaactgttg cagcagcaca tacttggggt cgctggcggc gatttcttcg gccttggcaa
     5701 tcgcgccctt catgcccttg gccccttcgg tcagcaccag tttggcgcca taagccagca
     5761 gcagtttgcg ccgctcaatg ctcatggtgt cgggcatggt cagggtaatc ggaatgccgc
     5821 gcgctgcggc cacaaaggcc agcgctatgc cggtgttgcc gctggtgggt tcgacgattt
     5881 ccttgccagc gccgagcagc ccgcgctttt cggcatccca gaccagtgct gcgccaatgc
     5941 ggcatttgac cgaataggcc gggttgcggc cttctatttt ggccagcacg gtggcaccag
     6001 cgccgtcggt gatgcggttg agcttgatca gcggggtgtt gccgatcgag agcgagttgt
     6061 ctgcgtaaac atgtgtcatg gtgcgtcctt ggtgtgattg aaatcgaatt aatcctgaac
     6121 ccagggcaag ccgtgtgagc gccagccacc cagatcgccc cggtggcgtt gctcatcaat
     6181 ctcgccttca aagccttcga gcacattgaa taccctggta aaaccggcct tggccgcagc
     6241 gtcggcagcc aggtccgagc gcttgccgct gcgacacagc agaaacaccg tggcatcctt
     6301 gccggttttg gcttccagtt cgcgcacaaa acgcgggttg cgggtcatgg ccgtgccgct
     6361 ggcccaggcg acatgcaggc tacccggcac atggccgaca aatttgcgct cctcggtggt
     6421 gcgcacatca accagcaccg cttggcccgc gctgaccagt tcccaggcca gttgcggtgg
     6481 tacgccaccg gcatagctca ggccggtggc cagggccacc gagcggatgg attcaagtgg
     6541 atcagccagg ctgtcggtgg tgtttgcagt gttcatcagg aaatgccttt catgtctcgt
     6601 ggtatggatc tttttaattg attttgatgg ctatagacgg ttgaaaggac tttaatgccg
     6661 gactgccaaa tatcaaacga ctaatttctc agttcgatat gagaaaacct tctaaagatg
     6721 tggtcgggaa taactccccg ttttgcacca aggagttggg atgcacggct aggcgcaaga
     6781 cgcgccgttt agctggctaa acaagcgtct tgcaacgacg ctgtgcgccc aaatccgccg
     6841 gtgcaaatag gggaagttat ttctgaccac atcctaagtg gaatcgagtc ttgctttcgg
     6901 gtagctcacg cggtcaagcc aggcttgcaa tgccatgccc agcagcaaag ccgcaagcag
     6961 ccaaaacagc tcggaattcc cgaaccccag cgatgcaagg gcgggtcctg ggcaatagcc
     7021 agcaattccc cagcccatgc caaagatggc cgcaccggtc accaggcggg catcgatcaa
     7081 agcggactgt ggcaggtgaa aacggctgtc gaaccggggc gatggcttgc gcaaaatgat
     7141 gtgaaaggtc gccgcactca agatcaccgc agctcccaga acaaatagca gactggcatc
     7201 ccagtggcct gtgatatcca ggaaactcag taccttattg ggatcggtca tttgcgcaat
     7261 ggccagaccc aggccaaaca ccgtaccagc ggccaggctg ctggctcctt gcaacagtga
     7321 ccgagaaaga ctcatgaact gaccccaaca atatgacgca ccacataggt ggtcgcgatg
     7381 gctgcgatca gtaacgtcgc tgtggctacc aatgatcgca aggacaggcg cgccaagccg
     7441 catacgccat ggccgctggt gcagccgttg gctcgagccg agccaaagcc tgtcaagaaa
     7501 cccgcaagca gcagcaaata caccggaaag cccagacgga tcggtgccgt gacaccggtg
     7561 acaagatagt agccgccacc gccaagcacc aggccggcca aaaaccagcc gcgccaggca
     7621 cggtcacccg aacctggcca gagcagtccg cccagcaccc cgctgatgcc ggcgatgcgg
     7681 ccattgagcc agagcagcaa actggctgac aagccaatca gcaagccacc gaccaaggct
     7741 gaaacaggcg tgaaatcagt catgacttgg gtgtttgcgc aactgcatca gaacttccag
     7801 ccccaggaaa tcccgatgat gttttcctta agccggacat tggcgttgcc gccgccaaaa
     7861 cccggcggat tgccgggcgg aatcgagcca ctgccattga ccgtttcacc cacgccatag
     7921 gcataaaaac cgctcaactc gccaccgccc ggtgtcgtcc aggtggcacc cagcgtcagg
     7981 tgccgggtga tcacgccggg ggccagcacg ttgaaaaagg tctcggatga aggcaccggc
     8041 tggctggcaa aactgagtcc gccgcgcaag ctaaggtcgg gtcgcaaagt gtggctgaca
     8101 ccgagcttga gcacgttgac atcgcgccag ccaaagcccg gcccattggc agcccccaga
     8161 gccgactggc cggcaaacag gccggccgcc gagttgccga cagagcctac ctggctgtag
     8221 cgaatggttt gcacatcggc ggccagcgtc caggctggtg tggcctcaaa ggccaggccg
     8281 acaccatagt tttctggcac atcaaagccg ccagcatcgg caaacaggcc cttgtacttg
     8341 tcaaatttgc ccgatatttt ggaggcccag gtggcaccca gcgtcagttg cggggttatt
     8401 ttgccaagcc agcccagccg cacgccgaca ccggtgctgc cgtcaacgcc ctgatcgctg
     8461 acattcgccg gggcgctcga aaagccacca aacaggccga tgcctttggc ctcaaaacgt
     8521 tgataggcca gggtaagcgc tgcgccaaag gtgtgggcct cgttgacctt gaaggcaatc
     8581 gagggcgaaa taaagagctg ttccaggttg atgccggccg aaccggtggc gccaaaacgg
     8641 gcatacgggt tctggccata gtcggtgttc atgccgccgt tgccaaaaac ggccagcccg
     8701 atggcggtgc tggggttgag ctggcgcgtg tagccaaaat ctgggatgag aaaggtcttg
     8761 ctatcgtcgc cgctgtaact ggcatttggc ccaaaggcat tgcccgcgat gtcggctccc
     8821 cgactcgggg caaataccgt cagccccagg tcaaggcggc tgcccactgc ggcagtgccg
     8881 gccgggttgc tggccgccgc cagaccgtcc tgtggcaggg caatgcctac gccagcgata
     8941 ccttgcgact tggcaccata gccgtgcgaa aaatagccat tggtggcact ggcttgcagg
     9001 ggtaccagca gcgctgtggc aagggttgcg gcgcgaaggg aaaactcgaa ggtgcgtgcg
     9061 gaggtgctca tggtgtctgc caggcaaagg gtgggggcaa gtggcaacgc cacctgtcaa
     9121 gccttcactg tgcggcatga ccccggtaaa gccaacgaag atattcgcat aagctaaaac
     9181 ggttttgttt gctgaccctc tgatatatag gccattttgg cctgtagcca ccgtccttat
     9241 tgcgctggaa gctatcaata gcatagtgcc tttgtgctct gaccaagccg ccttgtgggc
     9301 gcttatgcat tttttaagaa tctatgcttg aaatccatcg ttcccaaatg gctggcccct
     9361 gtctagcatc tgcggctttg atgtgaaggg tgaaccatgc caaagcctta caacccgact
     9421 tacgatccgc tgctatcaaa ggatcttggc gcagggcttg accatgcccc gacctactgg
     9481 accgccagcg cgggtgcgct gccatcagac gacggcccga tcactgccga caccacggcc
     9541 gatgtggtga tcattggctc aggctcgacc ggcatcgcca ccgcgctgta cctggcgcaa
     9601 gagcatggca tccgggcatt ggtgctggag gccaaccaga ctgtctgggg ttgttccagc
     9661 cgcaatggcg gccagggtca aaacgccgcc gggcggctga cgcgctcgca gtggatagcg
     9721 cgctgggggc tggcaatggg gtggcgtttt ccatctgggc tggcaaacgc ctggccgaac
     9781 gggtagcggg caaagatgct ggccaaaagg tgtttcagtt gccgatttac gcccagccac
     9841 tgccagcacc gaatctgttt gggctgatat gctcgcccgc gctggcaccg tttcgccgtc
     9901 tgggtcagcg catgttgtac cagtggtatg gcttgcggga tcagtgaggc ggctgccgca
     9961 tgcacagatc gggctttaaa aacccgttca gtgccgccag catctgctct ggtgtctcgt
    10021 tcatctgttg gtggccaccg ggcagcatcg caacctgggc gtcatgtgct ttcttgatca
    10081 ggccctgcgc cgctttgggc ggtgtcatct ggtccatgct gcccagcaca aacagcaccg
    10141 ggcaagtcac ctgcgccatc gccgtctcgc cattggcgta gctgtcgcag gccgcaaagc
    10201 cggtgtagaa gacgttgacc tgcgggttgc tggccagcac ccggcgcccg agtgccagcg
    10261 atgcgccata aacccaggtg cccggcccca tcgccgaggg cggcggtgcc agggttgagc
    10321 gcgaaaaaac attgaccatt tccagcgcct tcatcggctc gctctgcgac gctgccagca
    10381 acgcatgcga cactttcatt ggaaaagcca cgcccaccag caccagttga ctgacccgct
    10441 cgggcgcccg tgcggccgcc tccagcgcaa tcagcgagcc caggctgtga ccgatcaaga
    10501 cggcttgggc cagaccggcg gcatccatca gggcgatgat gaaatcggcg gcttcttcga
    10561 cgctggcagg cggctggccg gcgctgcggc agtggcccgg cagatcgacc gccagcacat
    10621 tccagccgtg gtgcgccaga tagcgggttt gcaaaatcca gacgctgtgg tcgttcaaca
    10681 cgccgtggat gaagatgatg gcgggcttgg ccgcatcaaa cgctttgcca ccggtgtagc
    10741 agtaggtggc ggcttggttg acggtgaaat acatggcagc gcctgttgaa tgtgaaaata
    10801 tgtgtcaaat cgggctgtag cccccgtgga atatgcgcaa gcagctataa cttaaatagt
    10861 aaccaggaag cgctgcaaag ttggcttcag gcccgttcgg ccgccttgag ggcgcgcttg
    10921 aggtcgtcaa tcagatcgtc ggcatcttca aggccaatgc tcaggcgaat cgtgccctgg
    10981 ctgatgccgc ctgcggccag cgcttcgtcg ctcatccgaa agtgcgttgt gctggccggg
    11041 tggatcacca ggctgcggca gtcgcccaca tttgccaggt ggctgaagac cttgaggctt
    11101 tcgacaaact ttttgccctg ctcgcggctg ccgcgcaagt cgaagctgaa caccgaaccg
    11161 gcaccgcgcg gcagcagcct ttgcgccagc gcatggctgg ggtggcttgt cagcagcgga
    11221 tggccgacgc gtgccacaaa gggctgactg gccagaaagc cgacgacttt gtgagtgttt
    11281 tccatgtgcc gcgtcatgcg cagtgacagc gtctcgatgc cctgcaaaat cagccaggcc
    11341 gaatgcgggc tcaagcaggc gccaaagtcg cgcaggccct cgcgccgggc ccgcagcaag
    11401 aaggcaccca cggtggattc ttcggtgaac accatgttgt gaaagccagc atagggctcg
    11461 gtcagttcag ggaatttgcc gctgcgctcc cagtcaaaac tgccagcatc gaccacgatg
    11521 ccgccaatca ccgtgccgtg gccgctcagg aacttggtgg cagagtgata gaccagatca
    11581 gcgccatggt caaacggcct gatcagccag ggcgaggtca gcgtcgaatc caccagcagc
    11641 ggcacaccgg cttcatgggc gattgaactg acggcggcga tgtcaagcac ctccagccca
    11701 gggttgccga cggtttcgcc gaacaacagc cgggtattgg ggcgcacgcc agcgcgccag
    11761 ccgtcgatgt cgccgggttt gacgaaggtg gtgtcgatgc cgaagcggcg cagggtgtag
    11821 tgcagcagat tctgcgagcc accatacagc gcggtgctgg ccacaatgtg cgcgccagca
    11881 cccatcagcg tggcaatgca caggtgcagc gcggcctggc cgctggcggt gctgatggca
    11941 ccaatgccgc cctccagcgc ggccacccgc tgctccagca ccgcgttggt cgggttgctg
    12001 atgcgcgagt aaacgtgacc ggcccgttcc aggttgaata gcgacgccgc atgctcactg
    12061 gactcgaaga caaacgaggt gctgaggtaa atcggcacgg cgcgcgcgcc ggtggtgggg
    12121 tcaggcgcag caccggcgtg cagtgccagc gtgtcaaaac cagggtctga atagccgggc
    12181 atgggtgtcc ttttggtaag tgctgcagta catcacaaag tgccaatcac attggttata
    12241 tccatcgttg ttatgcaaat tatggattca agatcatcgt ttgaatgtct tgaatgaacc
    12301 gtaagcgagc attttgatac ctgctgccca gcgcctgccc gcccttccca gcgaccagaa
    12361 attctgcgac gcggtgctcg aaacacggtc acgcgccgat tgggatgcct tgaagctgcg
    12421 cctgctgcaa cggtaccagg cccacgccca ggtcgcagac ggccgacctt tgcatgacaa
    12481 attccagtca gtcaaattgc tgccgctatc catcatttgg atatgggctt gtacgcatcg
    12541 gcatggcctg aagagtaagc cgtagggcag taagatcgcc aaaatttgaa agggactcgg
    12601 tatgaatctg atcattagac gcttgacgcg tggttcgtcg caccgcctgg ccgcattgtt
    12661 cctctttctg gccgggcttc aggttcccgg cctggcgctg gaggcacctt gccctcccgg
    12721 caatgcctcg gtctgggggc cagccgccaa ggacttcctc ggaacgtccg ccagcaacaa
    12781 ctcgcgcgtt tatttcaccg gtgccgaggg catcctgacc gaggtctttt atcccacgct
    12841 tgaccgggtg cagaacgtcg atctgcaatt gctggtcacc gatgctggca aaacctgggg
    12901 cgacgaggag cgccgacaaa agcagcgcga catcagccag gtcaatcgtc gcgccatgtt
    12961 gtggcagacc gtgacgacag ccgacaacgg caagtggaaa atcaccaaga aaatcttcac
    13021 cgatccgtgg cgcaatgcac tgatccagcg cgtcagcttc cagacgctgg aacccggcaa
    13081 gaaagtcaag gactacaacc tttacctgct gaacaatcca gccatcaaca acaccggggc
    13141 gggaagcggc accagcaatc aatgcagcgg ccggcagggt accgcgcagg gcgctgacaa
    13201 ctcgcgcacg ctgagcgcca ataaccgcat catgctggtg gcatccgagc cgaattcaac
    13261 ggcctcggcg ctggcggttt cggtgccatg gaaaacggtg ggtggccagg cgatggtgtc
    13321 gaacggtttt gttggccgca acgatggctt caccgatctg ttcgccggtg ccaatgacag
    13381 aaccatgaac ttccgctttg atggcgcttt tggcggcaac gtcgcacaaa tgggctggat
    13441 cgatactggc ggcaataccg acaccagcct gtctttcgat gtggtgttgg ctttcggcgg
    13501 cagcgaagca gaagcaatgg gtgtcgccaa tgccacactg ggcaccagcc tgaatgcgct
    13561 ggaacaggtc tatatcaacg agtggctcgc ctacagtcag acgctgaaca atcaaaatgg
    13621 acgcgccgac gatcagtact acctggcggc aatgaccttg aagagcattc aggacaagag
    13681 caacggcgcg atgatcgcgg gactcggcac accgtggggc gaaaccagca acgacggcaa
    13741 tccgggcggc tatcacctga tctggtcgcg cgatctgttc aagtttgcca gcgcgctgat
    13801 cgcggcgggc gataccgatt ccgccaaccg gacggtcgat ttcctgttca accggcagat
    13861 gcagacgagt tctggagata acccctattc gcgtcccggc cgcttcccgc agaacagctt
    13921 tgtcgatggc acgccgtact ggaacggcac ccagatggac gaaaccgcga tgccgatcat
    13981 tctggcgtgg aagctcggtc gggttgatct gtggccgaaa atcaagctgg ctgctgaatt
    14041 tctggcgcat aacggcccgg caaccgggca ggagcgctgg gaggaaatgg gcggctattc
    14101 gccgtccacg attgcggctg aaatcgccgg tctggtttgc gccgccagcc tggccactgc
    14161 ggcaggcgat cccggcgctg ccagtttcta tctgcaaaag gctgacgagt ggcgcaataa
    14221 cattgccaac tggaccttca ccacaactgg ttttcatacc ggttgtaatg ggtgcaatgg
    14281 caaatactac atccgcatca atgccaacca ggacccgaac gatgatgtca acctgacctt
    14341 cggcaacggt gtcggcacgc atggcgaacg ctacatcgtc gatggcggtt tccttgaatt
    14401 ggtgcgcatg ggggtgatga gcccaagcga ctggaccatc cttgacaccc tgcctgaata
    14461 cgatgcgctg ctcaaacaaa ccatcaccgg caagggcgat gcctggtttc gctacaacta
    14521 cgatggctat ggcgaataca acgatgggcg caattacgac ggcagtggtc gcggtcgcct
    14581 gtggccgatt ttcaccgccg aacgcggcat ctacgagatc gccaaagcca gcgacagctc
    14641 gaagggtgaa ccgtaccgcc tggcgctcaa ggcgttctcc tcgcccgcag gattcatccc
    14701 ggaacaagtg tggaacaaca gcgccaatat caccggctgg caaaccgata cgccgtcgtt
    14761 ttatgcagtg ggcagcgcaa ccaagtcgat gcaaccgctg agctgggcaa tgggcgaata
    14821 catcaacttg atcgcggcga tgaacctggg gcaaagcgac gcgccgactg tcgtctgcca
    14881 acgctatgcc tgtgataaac cgcagagcac cgtgaccttc agggtcaacg ccacgaccag
    14941 tttcggcgaa aacatctacc tggtaggcag cagtcccctg ctcagcaact ggctacccgc
    15001 ctcggggatc aagctgtcgc cagcccagta cccggtctgg aacgtgacgc tgtcgctgcc
    15061 cgccggcatg gcgctggagt acaagtatgt caagcgcgat gcgggcggca atgccgtctg
    15121 ggagagcggc agcaaccgca gttttgccgt gccagccagt ggcgaacttg tccacgatga
    15181 catatttaaa ccttgattcc tcagttggac gcgcccgagt gggccgctgg cggcgcgtcc
    15241 gggtagggca aaagccgcgg ctccaaacgt gcctgcgcac gtttggacgg cttga
//
LOCUS       DAOSLL010000024        15295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4011_length_15295_cov_4.551378, whole genome shotgun sequence.
ACCESSION   DAOSLL010000024 DAOSLL010000000
VERSION     DAOSLL010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 15295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15295
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4011_length_15295_cov_4.551378"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="PLB25_01815"
     CDS             complement(<1..485)
                     /locus_tag="PLB25_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40361.1"
                     /translation="MFCVLPYRLNTSGRVGPDQHVAAATGFCCAATPLRVSRSSWQSS
                     HRQGAAERTRKNSLVKQMIMISNLKSKLLSASLIACFSSVVLAQALPEAMVSAARKAV
                     VSNPEVQARWNGFNAADNERQVARGGFFPQIDITANVGRENRETPVADYGTYNYNTAQ
                     IT"
     gene            841..1440
                     /locus_tag="PLB25_01820"
     CDS             841..1440
                     /locus_tag="PLB25_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006223939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HOE40362.1"
                     /translation="MASIWATPDLDKMQILALQRYGAPAAETVSAWRKLMVEARALPE
                     LEKLNKVNVFFNRRVLYETDLAVWQQEDYWATPLELMGRAAGDCEDFAIAKYMTLLML
                     GIGNQNLRLIYVRAKVGGATVAHMVLGFYPQPTEEPMILDNLITSVRAASQRSDLAPV
                     FSFNSEGLWAGGAVTSSADPTARLSRWRDVLERMRQDGL"
     gene            1486..3399
                     /locus_tag="PLB25_01825"
     CDS             1486..3399
                     /locus_tag="PLB25_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE40363.1"
                     /translation="MSLIKQLWIAIAMVMTLAFGVSMVVSVLSARHYLEQQLQVKNID
                     NATSLALSLTQLPKDEVTVALQVAAQFDAGHYRFIRVLSPTGKVLVEKLATSHQMGAP
                     AWFAKLIPIHAEPGQALIQDGWIQYGTLTLASQDVFAYKSLWDGMLELLLWFIVGSVI
                     TGVVGTQLIRIITRPLAEVVDQAHAITERRFVTIKVPRTPEFKSVASAMNDMVGRLKA
                     MFNEEAARLDALRKKVNRDAVTGLSSREYFMSHLREVVQGDQFGPSGSLVMVRLADLN
                     ELNSSLGRQQADMLLKDVGAELYNSGKERIGQRAGRVKGAEFAIICPSFETAREAAQD
                     IHQRLTQHVLSKWIGQVPSLFCVSAVRYHRSQNVGNLLSAADEALAQAANKGPNAWHA
                     VEDGNVKVAVPAEQWRTLLTEAVSGGRLVLNFFPVVAGDGVGALHTEGVIRLQADAAG
                     TLMPAGDFMPMAAQLNLTAPIDLGVVKLAIDRLQAISGDVAVNLSAETIADFHFRNEL
                     TLLLKAYPEVCKRLLFEVPEYGVFRQFDAFCELVRSLKQLGCRVGVEYFGQRFAESDK
                     LADLGLDYIKVHPSYVRGLADNPGNQEFLKGLCKVAHSIGITVVALGVQSRDDLPLLA
                     SLGFDGATGPGIQ"
     gene            3396..4364
                     /locus_tag="PLB25_01830"
     CDS             3396..4364
                     /locus_tag="PLB25_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOE40364.1"
                     /translation="MTPRFVRSALAACVCALGFPGGQALAQVRMPGPASQAVLEDAPP
                     AVRQILQQASDIEAAARPDAAWLTAALYCQAARWGSAEGQYRLGMLYAFGQGVPESRA
                     YAAALFSVASALGHAQAHQMLDTIEFTSAELPACVNHEQMPEKAPAGTHAFRGLDLKI
                     DANPGIDLYLLKMPANKRWIVPLATTLSAWYALDPKLVLSVIAVESNFQTGVQSPKAA
                     VGLMQLIPDTAERFNVRNAYDATQNMRGGMRYLRWLLSYYRGNVPYALAAYNAGEGRV
                     NQHKGVPPYAETRAYVKRIVGLYGGVQHMYDATLTTPPPWLTVGSK"
     gene            complement(4870..5595)
                     /locus_tag="PLB25_01835"
     CDS             complement(4870..5595)
                     /locus_tag="PLB25_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40365.1"
                     /translation="MQASKIIREAMTRVAALRQVAGNQPTLAQAVLDVKHLQAQRFAA
                     TYADLLDSPAYAPSARFFLEELYSARDYSLRDQQFARIASALEFTFPDHVVAMAVALA
                     QLHGMTEELDLAMGQAWLQYQAEPAAKRYVAAWHHVGRPADRDWQLATVLSIGEKLTE
                     LTRKRSLRVMLKMMRKPAKLAGLDSLQAFLETGFDRFAGMACQGGTARVFLDTVKSRE
                     TAWIFQLFHSPDNAGNTEVFGTK"
     gene            5710..6438
                     /locus_tag="PLB25_01840"
     CDS             5710..6438
                     /locus_tag="PLB25_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE40366.1"
                     /translation="MSEIMSKPHDNATQVSTLPHKVAESGRSLPAVPNATSLVVPIRS
                     LGENHRSRISNHLLSLDPRDRYYRFGFAAKDEQIHRYVDGLNFERDEIFGIYNRHLTL
                     IAVAHLAFSIDMSRNPCAEFGVSVLPKARSRGYGGRLFERAVMHARNRGVQTIFVHAL
                     SENTAMLKIARHAGALVERDRSESEAHLSLPPATLNTHLTEIVEEQMAQTNYQFKVHV
                     KRFYDTLDRLQAVWQATGPADRAS"
     gene            6532..7398
                     /locus_tag="PLB25_01845"
     CDS             6532..7398
                     /locus_tag="PLB25_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HOE40367.1"
                     /translation="MSDPYPPRPGREDNRTFLQKIAEFIHPGPDSTEELIDWLTEAER
                     NHIIGDDSRKMLEGVIRMADMTAGDVMVPSTRMELVNIEDPHDKMMHGVIDTAHSRFP
                     VFEGERENIIGILMAKDLLKLQRAPELNIRALLRPAVFVPESKVLNDLLRDFQSNRNH
                     LAIVIDEFGRVAGLITIEDVLEQIVGEIEDEFDIAEDEGDIYGLADGTHRVSGDTLIE
                     RVEHAFGVKLASTDPDDTFETIGGLIAHEMGHVPKRGERFAFAGLDFAVLHTKGGAVK
                     WFKVAPVSDASA"
     gene            7385..8998
                     /gene="lnt"
                     /locus_tag="PLB25_01850"
     CDS             7385..8998
                     /gene="lnt"
                     /locus_tag="PLB25_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HOE40368.1"
                     /translation="MQARNSSAGFDLFVRQPQASRWWSVGLVLLAGALQAASVAWPLS
                     LPDALAGLGLQEGQPVWWLQTGAMALLVHLLLGSASVRQTAMLGWLFALTWLSLTFAW
                     LYTSMHTYGGLAAPLAGLAVLGLAAVLALYYALICGLFRALALMGSVLEAMVFAALWL
                     LAELARGVWLSGFGWGAVAYAQIDGPLAPLLAWVGCYGVGFAAAALAALLALAVRADN
                     WRKRLVSLLVVLVAAGLCAHFPAPAGADAGTLSVTLLQGNIAQEEKFESATGVPAALR
                     WYGDQLQASRSALIITPETALALLPEQLPDGYWHALQQRFATGEQAALVGLPLGSFTE
                     GYTNSVVGFKPGQASAWRYDKQHLVPFGEFIPPLFRWFTNLMKIPLGDFNRGAPVQPT
                     FDWQQQRLATTICYENLFGEEMARHFADPVRAPTLLVNISNLGWFGERLAMDQHLQIA
                     RARSLEFDRAFLLATNTGRTAIVDHLGRVTHALAPHTAAILSAQVQGRTGITPYAFWV
                     SRWGLWPLWSLAAAVLLAAALSGRHRLRR"
     gene            9403..10317
                     /gene="glyQ"
                     /locus_tag="PLB25_01855"
     CDS             9403..10317
                     /gene="glyQ"
                     /locus_tag="PLB25_01855"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HOE40369.1"
                     /translation="MLTFQQIILKLQSYWDAQGCALLQPYDMEVGAGTSHTATFLRAL
                     GPEPWKAAYVQPSRRPKDGRYGENPNRLQHYYQYQVVLKPAPSNILELYLGSLEALGF
                     DLKQNDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCRPITGEITY
                     GLERLAMYLQGVDNVFNLTWTEAPDGTRLSYGDVYKQNEVEQSTYNFEHSDADFLFTA
                     FTAHEKQAKHLMQAQLALPAYEQVLKCAHSFNLLDARGAISVTERAAYIGRIRTLARG
                     VAQSYFDSRKRLGFPMAPRAWVAQMADV"
     gene            10537..12786
                     /gene="glyS"
                     /locus_tag="PLB25_01860"
     CDS             10537..12786
                     /gene="glyS"
                     /locus_tag="PLB25_01860"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HOE40370.1"
                     /translation="MTSQNLLVELFVEELPPKALKKLGEAFARLLADQLRGLGLASPA
                     SVVTSYASPRRLGCHITEVAAKAADKVVTQKLMPVNVGLDAQGVATSALLKKLQALGA
                     DVSDPVFAVAALRKAPDGKAEALFFDNLVGGATLDLGLQKALDETLAKLPIPKVMSYQ
                     LESDCELPGWSSVNFVRPAHGLVALHGSAVVPVKLLGLTAGNTTQGHRFEALKSPVFI
                     ANADDYAASLLADGAVVASFAERRADIARQLAAAASQVSGDCHPIEDDALLDEVTALV
                     ERPKVLICQFEPQFLDVPQECLILTMKANQKYFPLLDGGNKLTNQFLVVSNINPADPS
                     AVIGGNERVVRPRLADAKFFFDQDRKKSLESRVAGLDKVVYHNKLGTQGERMARVCVI
                     AQNIAALMLQAGGPMMPADLLAKVELAARLAKTDLLTDMVGEFPELQGIMGAYYARHD
                     GLSEDVAQAIEDHYKPRFAGDDLPRSLTGVVVALADKLETLVGMFGIGNLPTGDKDPF
                     ALRRHALGVIRMLVENDLPLSLDTLIERAAIAFGAQFLVTPAQPTGRVSEKPGTGFGT
                     PTAISDVPMDGKAGRLLADFIYDRLAGSLREQGYSAQEVDAVLALKPQRLGEVPKRLA
                     AVRAFAALPEAPALAAANKRISNILKKADTVADAHVSELLLNEAAEVALYAAMQDIAP
                     RALARFDAGDYTGALQTLAALRAPVDAFFEGVMVNADALDLRMNRLGLLKTLFEAMNR
                     VADLSRLAA"
     gene            12837..13406
                     /gene="gmhB"
                     /locus_tag="PLB25_01865"
     CDS             12837..13406
                     /gene="gmhB"
                     /locus_tag="PLB25_01865"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HOE40371.1"
                     /translation="MNPIKLVIVDRDGTINQDSVEYIKSADEWQALPGALEAVARLNH
                     AGWHVVVATNQSGLGRGLFEVSALNDIHTKMHQQLGALGGRIDAVFYCPHTASDVCSC
                     RKPASGLFEQIAERYGISLKGVPVVGDSLRDLQAGAAAGCEPHLVLTGKSDMLRGQSV
                     PDSFPPHTVAHEDLAAFVEFFLTREAQSL"
     gene            13506..14240
                     /locus_tag="PLB25_01870"
     CDS             13506..14240
                     /locus_tag="PLB25_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE40372.1"
                     /translation="MALIRSVLHMVWMVVTVMPWTLAVLLVSLVAPRSAAWWTAVNWF
                     RVVMWGTRVILGVRMEVLGMDNLPVGKGSAAVLLAKHQSALETLWLPTLMQHPLAFVF
                     KRELLKVPFFGWSMARLDMIHINRESRTVAMKHVIAQGRRLLAQGTWVMMFPEGTRIA
                     RGQEGTYQTAGTRLAVEAGVPVVPIAVATARCWPRDGFVKHAGVVRVSIGPQMPSEGR
                     DPKALMRDAQAWIEAEMRRIDPEAYC"
     gene            14262..15191
                     /locus_tag="PLB25_01875"
     CDS             14262..15191
                     /locus_tag="PLB25_01875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HOE40373.1"
                     /translation="MHPLLRFTLDIFEPNRPLAHTDKAPVAIKKEADFGHPRANRQVR
                     LGQLLVDYEFNRGQRRSIGFCVGPDGLAVRAPKWVTLRDVDDALQAKASWILRKLQAS
                     RERLTRQASQVIDWRDGVVLPFLGQNLVIALDTVHRFGARGAHLVVESGPGQHPAALT
                     ETATPASASADSTPGQHAASRQVLYLALSQQATPEQIRAATQAWLMCHARHVFQQRLD
                     HFAPLLGVQWKKLALSNASTRWGSARADGTIRLNWRLLHFSLPVLDYVVAHELSHLRA
                     MDHSPRFWDTVRSVVPDYAALRKQLKSELVPRW"
BASE COUNT         2994 a         4372 c         4658 g         3271 t
ORIGIN      
        1 gtgatctggg cggtgttgta gttataggtg ccgtaatcgg caaccggggt ttcgcgattt
       61 tcacgaccaa cgttcgccgt gatgtcgatt tgcggaaaaa aaccgcctct ggctacctgg
      121 cgctcgttgt ccgcagcgtt gaaaccgttc cagcgcgcct gcacttcagg attgctcact
      181 accgccttgc gggcggcact taccatggcc tctggcaaag cctgggccaa aaccacagaa
      241 ctaaagcatg cgatcaacga ggcactaagc agtttacttt tcaaatttga gatcatgatc
      301 atttgtttga ctaaggagtt tttgcgagta cgctctgccg caccttgtcg atgcgaactt
      361 tgccaagacg atcttgatac ccgcaacggc gttgctgcac aacaaaaacc ggttgctgcc
      421 gccacatgtt ggtccgggcc aacgcggccg ctggtgttca gtcggtacgg gagcacacaa
      481 aacatgcgtg ccacgacgat gtcgtacctt gagcaagcaa gtacctgttc agagccaacc
      541 ccggtggcca cggttctttg atcataaagt ctgtaaaggc cgccagcgcc tgtggcagtg
      601 ctataaattt cattttcgtg gtcacgccgt tctccagaac caagtaggcc tgcaactata
      661 agttgtttac agatttttac caatacctta ttaaaatcag atacagcagg ttgttttctg
      721 tcattcgaat caaaaggata gtcttacacc atgcgcgttt ttcgggccac gatccatggt
      781 atgtgttgcc cggcatttgg caggtttgcc aggtcgctgc tagccatcct ggtgctgctt
      841 gtggctagca tttgggcgac ccctgatctg gacaaaatgc aaattctggc gttgcaacgc
      901 tacggcgctc ctgccgccga gacggtgtcc gcctggcgaa agttgatggt cgaagcacgt
      961 gctttgccag agctggagaa actcaacaaa gtcaatgtct tcttcaaccg gcgcgttttg
     1021 tatgaaaccg accttgcggt ctggcagcag gaagactact gggccacccc tcttgagctg
     1081 atgggccggg cagcgggcga ctgcgaagat ttcgccattg ccaagtacat gactctgctg
     1141 atgttgggaa ttggcaacca aaacctgcgc ctgatctatg tacgcgccaa ggttggcggt
     1201 gcaacggtgg cccacatggt gctggggttt tatccccagc caacggaaga gccgatgatt
     1261 ctggataact tgatcaccag cgtgcgcgcc gcgtcgcagc gctccgacct ggccccggtc
     1321 ttcagtttca acagcgaggg tctttgggca ggtggtgccg tcacgtcatc ggcagacccg
     1381 acggcccgtt tgtcacgctg gcgcgatgtg cttgagcgca tgcgtcaaga tggcctgtga
     1441 gagctgcctg cctgcaaccc cattcaatca tctaccgaga ccgctatgtc gctcatcaaa
     1501 caactctgga ttgccattgc catggtcatg actcttgctt tcggggtcag catggttgtc
     1561 agcgtgttgt cggcccgcca ctaccttgaa cagcaacttc aggtcaaaaa catcgacaac
     1621 gccacctcgc tggccctgtc actcacgcag ttgcccaaag atgaggttac ggtcgcgttg
     1681 caggtggcag cccagtttga tgccgggcac tatcgattta tccgcgttct atcgccaacc
     1741 ggcaaagtcc tggtggaaaa actggccacc agccaccaaa tgggcgcgcc tgcctggttt
     1801 gccaagttga taccgatcca cgccgagcct ggccaagcgc tgattcagga tggctggatt
     1861 cagtacggaa cgctcacctt ggccagccag gatgtctttg cctataaaag cttgtgggac
     1921 ggcatgctcg aactgctgct gtggttcatt gttggcagcg tgatcaccgg tgtggtggga
     1981 acccaattga tccggatcat cacgcggcca ctggccgagg tagtggatca ggcacatgcg
     2041 atcactgagc gtcgctttgt caccatcaag gtgccgcgaa cacctgaatt caagagtgtg
     2101 gcgagcgcca tgaacgacat ggtgggccga ctcaaagcca tgttcaatga agaggctgcg
     2161 cgactcgatg cgctgcgcaa gaaggtcaat cgcgacgcgg taaccggcct ttcaagccgg
     2221 gaatacttca tgtcgcatct gcgtgaggtg gttcagggtg accaatttgg ccccagcggc
     2281 agcctggtga tggtgcgcct ggcggacctc aacgaactca acagcagcct ggggcgccag
     2341 caggcagata tgttgctcaa agatgtggga gccgagctgt acaacagcgg gaaggaacgc
     2401 attggtcaac gcgccggacg ggtcaagggt gccgaatttg ccatcatttg ccccagcttc
     2461 gagaccgccc gcgaagccgc ccaggacatc catcaaaggt tgacgcaaca cgttttgtcc
     2521 aagtggattg ggcaggtgcc gtcgcttttt tgcgtcagtg ccgtgcgcta tcaccgaagc
     2581 caaaatgtgg gaaatctgct gtcggctgct gacgaagcac tggcgcaggc tgccaacaag
     2641 ggtcccaatg catggcacgc tgtggaggac ggcaatgtga aagtggcagt accggctgag
     2701 caatggcgca ccctgctgac ggaagctgtc agtggcggcc ggttggtgct gaacttcttt
     2761 cctgtcgtcg ctggcgacgg tgtcggcgcg ctgcacaccg aaggcgtgat tcgcctgcag
     2821 gccgatgctg ctggcacact gatgccggcg ggtgatttca tgccgatggc agcacagctc
     2881 aatctgaccg ccccgattga cctgggtgtg gtgaagctgg cgattgaccg actgcaagcc
     2941 atttctggcg atgtagctgt caacctgtcg gccgaaacca ttgccgactt ccacttcaga
     3001 aacgagctga cgctcctgct caaggcctat ccagaagtgt gcaaacgcct gctgttcgaa
     3061 gtgcctgaat acggcgtgtt ccggcaattt gatgcttttt gcgagctcgt gcgcagtctg
     3121 aagcaactgg gttgccgcgt cggtgtcgag tacttcgggc aacgttttgc cgagagcgac
     3181 aaactcgccg atctggggct tgactacatc aaggtgcacc cgagctacgt tcgtggtctg
     3241 gcagacaacc ctggcaatca ggagtttctc aaaggactgt gcaaggttgc gcattcgatc
     3301 ggcatcaccg ttgtggcgct tggtgtgcaa agtcgtgatg acctgccctt gctggcctcg
     3361 ttgggctttg acggggcaac cgggccgggc atccaatgac cccccgtttt gtcaggtccg
     3421 ctctggctgc ctgtgtctgc gccttgggtt tccccggcgg gcaggcgttg gcccaagtca
     3481 gaatgcccgg ccccgcatct caagctgtgc tggaagatgc gccgcctgcc gtgcgccaga
     3541 ttttgcagca agccagtgac attgaggcag cagcccggcc agatgctgcc tggctaactg
     3601 ccgcgctgta ctgccaggca gcgcgctggg gcagtgccga ggggcaatac cgtctgggca
     3661 tgctttacgc ctttggccag ggggtacctg aaagccgtgc ctatgccgct gcgctgtttt
     3721 ctgtggcatc cgccttgggc catgcacaag cccatcagat gctcgacacc atcgagttca
     3781 cctcagccga gctgccagcc tgcgtaaacc acgagcaaat gcctgaaaaa gcgcctgctg
     3841 gcacacatgc cttcagaggc ctcgacctga aaattgacgc aaacccgggc attgacctgt
     3901 atctgctgaa aatgcctgcc aacaagcgct ggatcgtacc gctggccacc accttgagtg
     3961 cctggtacgc gctggacccc aaactggtgc tgtcggtgat tgccgttgag tccaacttcc
     4021 agaccggcgt ccagtcgccc aaggcagcgg tcggattgat gcagttgatt cctgacacgg
     4081 ccgaacgctt caatgtgcgc aacgcctatg atgcaaccca aaacatgcgt ggtggcatgc
     4141 ggtacttgcg ttggctgcta tcatattatc gtggcaacgt gccttatgcg ctggccgcgt
     4201 ataacgccgg ggaaggtcgg gtcaatcagc acaagggtgt gccaccgtat gccgaaaccc
     4261 gtgcctacgt caagcgcatc gtcgggctgt atggcggcgt gcagcatatg tatgatgcga
     4321 cgcttacgac accaccaccg tggttgactg ttgggtccaa gtaaacacgg ggtgtgaaat
     4381 tcctgtgcag cagtattttt tcagcgcgaa attttgcgat aggttgagaa cggcagagca
     4441 gtcaccatca ggcaagcgaa tggcagcgct tgcagccgca gggcagcgat gccatttgcc
     4501 ctgagtttga actggtgttg gccagcaagg gcaatagcct tttcaacacg ggtattctgg
     4561 ctcgcaagtt ctttgcctgg ctgcgcgtta cgtgctttgt ggactcgtcg aatccaccag
     4621 tgctggtact tgatgaagtc ggcagttact gtgcgacaag cggcagggat caagacctac
     4681 ccgctgaatc agcaaccaat cggcccgttc cagcgtgtcg gctttcaatg cacggtttga
     4741 aaacgccgtg gaagcggttt tggccactgc cgtcaattcg gtatttgcgt cagagcatgt
     4801 gcccgacggc cactgttatg cactgcgcaa atgtggacaa actaacctgg gggcgacccc
     4861 acgctgaatt tattttgtgc caaatacttc agtgttgcct gcattgtctg gcgaatggaa
     4921 aagctgaaaa atccaggcgg tttcgcgtga cttgaccgtg tcgaggaata ctctggcggt
     4981 accgccttgg catgccatgc cggcaaagcg gtcaaaaccg gtttccaaaa aagcctgaag
     5041 tgaatccagt ccggccagtt tggctggctt tcgcatcatt ttcaacatca cccgcaggct
     5101 gcgcttgcgc gtcagctcgg ttaacttttc gccaatgctc aacacagtgg ccagttgcca
     5161 gtcccggtca gcgggtcgcc ccacatgatg ccatgccgca acatagcgtt tcgcggcagg
     5221 ttcagcctgg tattgaagcc aggcctgccc catcgccagg tcaagttcct cggtcatgcc
     5281 atgcaattgg gccagtgcga cggccatggc aaccacatga tcaggaaaag taaactccag
     5341 tgcgctggca atgcgggcaa actgttggtc gcgcaaactg tagtcacggg cgctgtacaa
     5401 ctcctccaga aaaaacctgg cgcttggtgc ataagcgggt gaatccagca agtcggcata
     5461 agtggcggca aaccgctggg cctggaggtg cttgacatcc agcaccgctt gcgccagcgt
     5521 tggctgattg cccgctacct ggcgcagcgc ggccacccgc gtcatggctt cccgtatgat
     5581 tttcgacgcc tgcatttcac tgacagttta gttgctgctg tatcatgctc tgtgacggga
     5641 acccgacaaa caactggata agcgtaattt tagagttgag ccactaggga aaacccttac
     5701 atttaggtca tgtctgagat tatgtccaag ccccacgata acgccacgca ggtatcgacc
     5761 ttgccgcaca aagtggccga atcgggtcgt tcattgcctg ctgtgcccaa cgcaaccagt
     5821 ttggtggtgc caattcgctc tttgggcgaa aaccaccgca gtcgcattag caatcatttg
     5881 ttgtcactcg atccacggga tcgttattac cgttttgggt tcgcagccaa ggatgagcag
     5941 attcatcgct atgtggacgg gctgaatttt gagcgggacg aaatttttgg catttataac
     6001 cggcacttga cgctgattgc ggtggcgcac ttggcgtttt caatcgacat gagtcgcaat
     6061 ccctgtgccg agtttggtgt ttcggtgtta cccaaggcgc gtagccgggg ctacgggggc
     6121 cgactgtttg agcgtgccgt gatgcatgcc cgcaaccgcg gcgtgcaaac catttttgtg
     6181 catgcgctgt ctgaaaatac cgccatgttg aaaatcgcgc ggcatgcggg cgcgctggtg
     6241 gagcgcgaca gatcggagtc cgaggcccat ttaagccttc cgccagccac gctgaacacc
     6301 catttgaccg aaattgtcga agagcaaatg gcgcaaacca actaccagtt caaggtccat
     6361 gtcaagaggt tctatgacac gctggaccgc ctgcaggcgg tttggcaggc gaccggacct
     6421 gccgaccggg ccagttgatt cgtttcgcag ccgactttct tctgaccagc ctgctgctta
     6481 tcagctatcc ttgcggcctg atcaaccacc gcatgtcctg ccctaatggc cgtgtccgac
     6541 ccatatccac cgcgccctgg ccgcgaagac aaccgcactt ttctacaaaa aatcgccgag
     6601 ttcatccatc cggggccgga ttcgaccgaa gagctgatcg actggctgac agaagccgag
     6661 cgcaatcaca tcattggtga tgattcgcgg aaaatgctcg aaggcgtgat tcgcatggcg
     6721 gacatgaccg caggggatgt gatggtgcca agcacccgca tggaactggt caacatcgag
     6781 gacccgcacg acaaaatgat gcatggcgtg attgataccg cgcactcgcg ttttccggtt
     6841 tttgagggag agcgcgaaaa catcattggc atcctgatgg ccaaggacct gctcaagtta
     6901 cagcgcgcgc ccgagctcaa tattcgcgcc ttgctgcgcc cggcagtgtt tgtgccggag
     6961 agcaaggtgc tcaatgattt gctgcgtgat tttcaaagca accgcaacca tctggccatt
     7021 gtgattgatg agtttggccg ggttgccggc ctgatcacca tcgaagatgt actggaacaa
     7081 atcgtcggtg aaatcgagga cgaatttgac attgccgaag acgagggcga catctatggt
     7141 ctggccgatg gtacccaccg cgtcagcggc gacaccctga tcgagcgggt agagcatgcc
     7201 ttcggtgtca agctggccag caccgacccc gacgacacgt ttgagaccat tggcggattg
     7261 attgcgcatg aaatgggcca tgttcccaag cgtggcgagc gcttcgcgtt cgccgggctg
     7321 gactttgccg tgctgcacac caaaggtggc gctgtcaagt ggttcaaggt ggcaccggtg
     7381 tcggatgcaa gcgcgtaatt cgtcggcagg ctttgacctg ttcgttcggc aaccccaagc
     7441 cagccggtgg tggtcagtcg ggctggtttt gctggccgga gccctgcagg cagccagcgt
     7501 ggcctggcca ctgagcctgc ccgacgcact ggccgggcta ggcctgcagg aagggcagcc
     7561 ggtctggtgg ctgcaaaccg gagccatggc attgctggtg catttgctgc ttggcagtgc
     7621 cagcgtacgc cagacggcca tgctgggctg gctgtttgcg ctgacctggc tgtctctgac
     7681 ctttgcctgg ctttacacat caatgcacac ctatggtgga ttggccgcgc cgctggcggg
     7741 cctggcggtg ctggggctag cggcggtgct ggcgctgtat tacgcgttga tttgtgggct
     7801 ttttagggct ctagcgctta tgggtagtgt gctggaagct atggttttcg cagcactctg
     7861 gttgcttgcc gagttggcgc ggggtgtctg gctgagcggt tttggctggg gtgctgtggc
     7921 ttatgcgcaa attgacggcc cgctggcccc gttgctggcc tgggttggct gctacggtgt
     7981 cggctttgcc gctgcggcgc tggcagcgct gctggcgctg gcggtgcgcg ctgacaactg
     8041 gcgcaagcgc ttggtcagct tgcttgtcgt gttggtggca gcagggcttt gcgcccattt
     8101 tccagcgcca gccggggccg atgcgggcac cttgagcgtg accctgctgc aaggcaatat
     8161 cgcgcaggaa gaaaagttcg agtcggccac cggcgtgcca gccgccctgc gctggtatgg
     8221 cgatcagttg caggccagcc gcagcgcact catcatcacc cccgagaccg ctctggcgct
     8281 gttgcccgag caattgccag acggctattg gcatgccttg caacagcgct ttgccaccgg
     8341 cgaacaggct gcgctggtgg gactgccgct gggcagtttt accgagggtt acaccaactc
     8401 ggtagtgggt ttcaagccgg gccaagcgtc agcctggcgc tatgacaagc agcatctggt
     8461 gccgtttggt gaatttattc caccgctgtt tcgctggttt accaatctga tgaagatccc
     8521 gctcggtgac ttcaaccgag gcgcgccggt gcagccgacg tttgactggc agcagcagcg
     8581 actggcgacg acgatctgtt atgaaaacct gtttggtgaa gaaatggccc gccattttgc
     8641 tgatccggtg cgagccccca ccctgctggt caatatcagc aacctgggct ggtttggtga
     8701 acgcctggcg atggatcagc acctgcagat tgcccgtgcg cgctctctgg agtttgaccg
     8761 cgcctttttg ctggccacca ataccgggcg cactgccatc gtcgatcacc tgggccgcgt
     8821 cacccatgcc ctggcacccc acacggcggc tatcctgtcg gcgcaagtac agggtcgcac
     8881 tggcatcacg ccatatgcct tctgggtctc gcgctggggc ttgtggccgc tttggtcgtt
     8941 agccgcagcg gtgttgctgg cggcggcgtt atcgggccga caccggcttc ggcgatagtg
     9001 gcggggccgc gcccaagaag ccgtcggact tgtggcgcag cggctgcgaa ccgcccgcaa
     9061 caacccggtt gcaccggcct tttgatccat ggcggggtta tggcttgaaa tcaggcaccc
     9121 cgccactggg gtcgcttctc gctgctcgat ccctcagtcc gacaggctgc cggtggtcgg
     9181 caaaatcggc atcagcgcca tagtgcaggc ctgattccat ttccaaatca ttcgtatcaa
     9241 ggcaccctgg ggcagacatt gcaaacgcaa ggcaaggcga gataacaacg acatggatga
     9301 tttggagatg gaatgagccc ctaaaatcgg cttcaggcgc gctgtttctg gcgcgcgctt
     9361 ttaccaattt cgggtggcct cagacctcac ccccttcgct ttatgttgac cttccagcaa
     9421 atcattttga aacttcagtc gtattgggat gctcagggct gtgcactttt gcaaccttat
     9481 gacatggaag tcggggcggg aacctcgcac accgccacct tcttgcgggc gctcggcccc
     9541 gagccctgga aggctgccta tgtgcagccg agccgtcgcc cgaaagatgg ccgttacggg
     9601 gaaaacccaa accggctgca gcactattac cagtatcagg tggtgctcaa gccagccccc
     9661 agcaacatcc tggagctgta cctggggtcg cttgaagcgc tgggttttga cctgaaacaa
     9721 aacgacatcc ggttcgtcga ggacgactgg gaaaacccca cgctcggtgc ctgggggctc
     9781 ggctgggaag tatggctcaa tggcatggaa gtcacccagt tcacctattt ccagcaggtt
     9841 ggcggcattg attgtcggcc gatcaccggc gagatcacct acgggctgga gcgtctggcg
     9901 atgtacctgc aaggggttga caacgtgttt aacctgacct ggaccgaagc accagacggc
     9961 acccggctga gctacggcga cgtttacaag caaaacgagg tcgaacagtc cacctataac
    10021 tttgagcaca gcgacgctga cttcttgttc accgcgttta ccgcgcatga aaagcaggca
    10081 aaacacttga tgcaagcgca actggcgctg ccagcttatg agcaggtgct gaaatgcgca
    10141 cacagcttta acttgcttga tgcccgtggt gccatatcgg tgaccgagcg cgctgcctac
    10201 atcgggcgca tccgcaccct ggcacgtggc gtggcgcaaa gctattttga cagccgcaag
    10261 cggctaggtt tcccgatggc cccgcgcgcc tgggtagcgc agatggccga tgtttgatgt
    10321 caagcacatg gtcgtcaata gctttcccat ttggttcggc gggttgggcc gcactgtgtc
    10381 gctgcaaatc gctggtgtgg cttgccacat gttgcgcttt gcgtctggca ttgcatcttc
    10441 aatccagcgg ccaattgggt aacttgtttt cgaccgcatc ctgagcaact ttgcgcattc
    10501 gcaaatcgcc ctgtcttttc ccgaccagta actgaaatga cctcgcaaaa tctcctcgtt
    10561 gaattgtttg ttgaagagct gccgcccaag gcactcaaga aactcggtga agccttcgcc
    10621 aggctgttgg cagaccagtt gcgagggttg ggactggcct cgcccgcgtc agtggtcacg
    10681 tcatatgcct cgccgcgccg gctgggttgc cacatcaccg aagtggcggc aaaggccgcc
    10741 gataaggtgg tgacgcaaaa gctgatgcct gtcaatgtcg gactggatgc gcaaggtgtc
    10801 gcaacctccg ccctgttgaa aaagctgcag gcgctcgggg ccgacgtgtc tgacccggta
    10861 tttgccgtgg cggccttgcg caaggcgccc gacggcaagg ccgaggcgct gttttttgac
    10921 aacctggtgg ggggtgccac actcgacctg gggctgcaaa aggcgcttga cgaaaccctg
    10981 gccaagttgc caattccgaa agtgatgagc tatcaactgg agtccgattg cgagctgccg
    11041 ggctggagca gcgtcaactt tgtccgtccg gcccacggtc tggtggcttt gcatggcagc
    11101 gcggtggtgc cggtcaagtt gctggggctg acggcgggca acaccacgca ggggcaccgg
    11161 tttgaggcgc tcaagtcgcc ggtgttcata gccaatgccg atgactacgc tgccagcctg
    11221 ctggccgatg gtgcggtggt ggccagtttt gccgagcgcc gcgccgacat tgccaggcaa
    11281 ctggcggccg cagcaagcca ggtcagcggc gactgtcatc cgattgagga tgatgcgctg
    11341 ctcgacgagg ttaccgcgct ggtggagcgt ccgaaggtgc tgatttgcca gttcgagccg
    11401 cagtttctcg atgtgccgca agagtgtctg atcctgacca tgaaggccaa ccagaagtac
    11461 tttccgctgc tcgatggcgg caacaagctg accaaccagt ttctggtggt cagcaacatc
    11521 aacccggctg accccagcgc ggtgattggc ggcaacgagc gggtggtacg gccgcgtctg
    11581 gccgatgcca agttcttttt tgaccaggac cgcaaaaagt cactggagtc gcgcgtggcc
    11641 ggactcgaca aggtggtgta tcacaacaag cttggcaccc agggtgaacg catggcgcgg
    11701 gtctgtgtca ttgcgcaaaa tatcgctgca ctgatgctgc aagccggtgg gccgatgatg
    11761 ccagcggatc tgttggccaa agtcgaattg gcggcacgcc tggccaaaac tgacttgctg
    11821 accgacatgg tgggtgaatt tcccgagctg cagggcatca tgggtgctta ctacgcgcgc
    11881 cacgatggcc tgagcgagga tgttgcacag gccattgaag accattacaa gccgcgtttt
    11941 gctggcgacg acttgccacg cagcctgact ggtgtggtgg tggcactggc cgacaagctg
    12001 gaaaccctgg tcggcatgtt tggcattggc aatttgccca ccggtgacaa agacccgttt
    12061 gcgctgcgcc gacatgcgtt gggggtcatt cgcatgctgg ttgaaaatga tctgccgctg
    12121 agtctcgata ccttaatcga acgtgccgct attgcgtttg gcgcgcaatt tctggtgacc
    12181 cctgcgcagc ccacgggtcg tgtcagtgaa aagcctggca ccggttttgg gacaccgacg
    12241 gcgatctctg acgtgccaat ggatggaaaa gccgggcgac tgctggccga tttcatctat
    12301 gaccggcttg ccggttccct gcgtgagcaa ggctacagtg cccaggaagt cgatgccgtg
    12361 ctggccctca agccgcaacg cctgggcgaa gtgcccaaac gcctcgctgc cgtgcgtgcc
    12421 tttgccgccc tgcccgaagc accggccctg gcggctgcca acaagcgtat cagcaacatc
    12481 ctgaaaaagg ccgacaccgt ggccgatgcg catgtcagtg aactgctgct caatgaagcc
    12541 gctgaggtgg ccttgtacgc cgccatgcaa gatatcgcac cgcgcgccct ggcccgcttt
    12601 gatgccggtg actacaccgg ggcattgcaa accctggcag cgctgcgcgc gccggtcgat
    12661 gctttttttg aaggggtgat ggtcaatgcc gatgcgctgg acctgcggat gaaccggctt
    12721 ggcctgctga aaacgctgtt tgaagcgatg aaccgggtcg ccgacctgtc ccggctggcg
    12781 gcctgatgtt tgcctgatta ccccggcaac ccgatgtttc accacaggcc catgtcatga
    12841 acccgatcaa actggtcatt gttgaccgcg atggcaccat caaccaagac agtgtcgagt
    12901 acatcaagtc ggccgatgaa tggcaggcgc tgccgggtgc actcgaagct gtcgcccggc
    12961 tcaaccatgc cggctggcat gtggtagtgg ccaccaacca gtccgggctc gggcgcggcc
    13021 tgtttgaggt atcggcgctt aacgacatcc acaccaaaat gcaccagcag ttgggtgcac
    13081 tgggtggtcg catcgacgcg gtgttttatt gtccacatac cgccagcgat gtctgcagct
    13141 gccgcaaacc ggcatcgggc ctgtttgagc agatcgccga gcgctatggc atcagcctca
    13201 aaggtgtgcc ggtggtgggg gattcgttgc gcgacctgca agctggcgct gcagcgggct
    13261 gcgagccgca cctggtgctg accggcaaga gcgacatgct gcgtggtcag agcgtgcccg
    13321 acagctttcc gccacatacc gtggcgcacg aggacctggc ggcatttgtt gaatttttct
    13381 tgacgcgtga ggcgcagtcg ctgtgaccag ggaacccggt cagccacctg ggaacgccat
    13441 cagagtatga aaacgatagc tatccgcgct tatcgcataa gggctggagc ccaaaaagtc
    13501 atctaatggc gctgattcgc tcggtcttgc acatggtctg gatggtggtc acggtgatgc
    13561 cctggacgct ggcggtgttg ctggtgtcgc tggtggcacc gcgcagtgcc gcctggtgga
    13621 cagcggtcaa ctggtttcgg gtggtgatgt ggggcacgcg ggtgattctg ggcgtgcgca
    13681 tggaagtgct gggcatggac aacctgccgg tgggcaaagg cagcgcggcc gtgctgctgg
    13741 ccaaacatca gtcagcactg gaaaccctgt ggctgccgac cctgatgcag cacccgctgg
    13801 cctttgtgtt caagcgtgaa ctgctgaaag tgccattttt tggctggtcg atggcgcgtc
    13861 tagacatgat ccacatcaac cgcgaatcgc gcaccgtcgc catgaaacat gtgatcgcgc
    13921 agggccgccg tctgctggcg caaggcacct gggtgatgat gtttccggaa ggcacccgca
    13981 ttgcgcgtgg ccaggaaggt acctaccaga cggctggtac gcggctggcg gtggaggccg
    14041 gtgtgccggt ggtgccgatt gccgtggcca ccgcgcgctg ctggccgcgc gatggttttg
    14101 tcaaacatgc cggggtggtg cgggtgtcaa ttggtccgca gatgcccagt gaaggacgcg
    14161 acccgaaagc cttaatgcgg gacgcgcagg cgtggatcga ggccgagatg cgccgcattg
    14221 accccgaggc gtattgctga acattggcca ggcaactacc gatgcatccg ctgctgcgtt
    14281 tcacccttga tatttttgag ccaaatcggc ctctagccca tacagataaa gcgccagtag
    14341 ctataaaaaa agaagcagat tttggccacc caagggctaa ccgtcaggtc cgtctgggcc
    14401 aactgctggt ggactatgag ttcaaccggg gccagcggcg cagcattggc ttttgtgtcg
    14461 ggccggacgg actggcggtg cgagcgccca aatgggtcac attgcgtgac gtggacgacg
    14521 cgctgcaagc caaagcctcc tggattttgc gcaagttgca agcgtcgcgc gagcgcctca
    14581 cgcgccaggc cagccaggtg atcgactggc gcgatggcgt cgtgctgccc tttttggggc
    14641 aaaaccttgt catcgcgctt gacacggtgc accgctttgg tgccaggggt gcgcatctgg
    14701 tggttgaaag cgggccaggc cagcacccgg cagctctcac tgaaaccgca acgccagcca
    14761 gtgccagcgc tgacagcacg ccaggccagc atgccgcgtc acgtcaggtg ctgtaccttg
    14821 ccttgtcgca gcaggccacg ccagagcaaa tccgcgctgc cacgcaagcc tggctgatgt
    14881 gccatgcccg gcatgttttt cagcagcggc tcgaccattt tgccccgttg ctgggcgtgc
    14941 aatggaagaa gctggccctg agcaacgcca gcacgagatg gggcagcgcc cgcgccgacg
    15001 gcacgattcg gctgaactgg cggctgctgc atttcagcct gccggtgctg gactatgtgg
    15061 tagcgcatga actcagccac ctgcgggcga tggaccacag cccgcgcttt tgggacaccg
    15121 tgcgcagcgt ggtgccagac tacgcggcgc tgcgcaagca acttaaaagc gagcttgtgc
    15181 ccaggtggtg atgggcacca cctgctgcca gccagatcat cgcagccagt gggtctgacg
    15241 gccttaggat gtggtcagaa ataacttccc ctatttgcac cggcggattt gggcg
//
LOCUS       DAOSLL010000025        15108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4071_length_15108_cov_4.738192, whole genome shotgun sequence.
ACCESSION   DAOSLL010000025 DAOSLL010000000
VERSION     DAOSLL010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 15108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15108
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4071_length_15108_cov_4.738192"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="PLB25_01880"
     CDS             <1..454
                     /locus_tag="PLB25_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HOE40374.1"
                     /translation="IDNTAAKNYIARYFERYPGVKIYMDHTRQLAKAQGYVETVFGRR
                     LYLPEINSPNGPRRAGAERAAINAPMQGTAADLIKLSMVKVQQVIDAQHLATKMIMQV
                     HDELVFEVPMSEVDWVKHEVPKLMAGVAHLKVPLLAEVGMGVNWDKAH"
     gene            613..1284
                     /locus_tag="PLB25_01885"
     CDS             613..1284
                     /locus_tag="PLB25_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40375.1"
                     /translation="MENMGALAVAGCIISAAAVFVVMQFRRYEEQQDASKKILKATTD
                     FESTKTKLLGYTKFNDSLSAAKPHLVQLAKSLAANVVREYVHLERFGKDKHKLKSDVI
                     VIGKYTVEFCFAIDLKPDRFEIESEGAGISIKCGQPTLISPPSTKSASHEVSVPDVLA
                     DERATFIEVNQKFNELAQRYGLAVAREDAVRALCKAKLVDALRDFLAKEPGVRQIPTI
                     VVVFR"
     gene            complement(1500..1949)
                     /locus_tag="PLB25_01890"
     CDS             complement(1500..1949)
                     /locus_tag="PLB25_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOE40376.1"
                     /translation="MNPQNGYDVEDITVGMSAEIAKTITDADILLFSAVSTDTNAVHI
                     DEEFGRTTMFGGRIAHGMLSASLISAVLGNRLPGPGTIYMSQSLRFRAPVRPGETVHA
                     KVTVKEVIAEKARVVVDTVCTVGDKVVIDGEATLMVSSRKKREAAAK"
     gene            complement(2161..3138)
                     /locus_tag="PLB25_01895"
     CDS             complement(2161..3138)
                     /locus_tag="PLB25_01895"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HOE40377.1"
                     /translation="MRAVVCHELTGPSALRLMQVDDPRPRADQVLIRVRACGVNFADS
                     LITRGQYQMQPQLPFSPGFEVAGEVLELGAGVSKFAIGDRVIAMSAHGGYAEKVAADM
                     NRCVPMPAGMSWEHAAAFPVVFGTSHVALWHRARLRSGETLVVHGASGGVGLTAVAIG
                     KQLGATVIATANGPDKLAVAKAHGADHLLDASQEDLRMRIKELTGGRGADVIYEPIGG
                     DMFAASLRSIAFEGRILIIGFAGGVVPQIPANHLLVKNVDVIGVNWPAYAQLNPTVMT
                     ESFQTLIQWYLAGAIKPHVSAIYPLEQAVDALNQVLTRKSTGKVIITMS"
     gene            complement(3273..5027)
                     /gene="ccrA"
                     /locus_tag="PLB25_01900"
     CDS             complement(3273..5027)
                     /gene="ccrA"
                     /locus_tag="PLB25_01900"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR01751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HOE40378.1"
                     /translation="MSKQLYEVGEQPPLGLVPEKMHAWLIRPERFGKPMEAFQKEVVD
                     TPSIADDEVLVYVMAAGINYNNVWAALGIPVNVIAARNKNGEPEPFHIGGSDASGIVY
                     KVGKDVTNVKVGDEVVMHCGTFNRNCEWVKGGGDPMYSPSFKIWGYETNFGSFAQFTR
                     VQGQQCMPKPKHMNWEEAASYVLVAATAWRMLHGWESSSIKKGDVALIWGGAGGLGSM
                     AIQIAKAVGATSVAMVSGQDKFDYCMKLGAKGCINRNDFDHWGMLPHWKDNAGYGKWL
                     KGVRSFGAKIWEVLGEKRAPNLVFEHPGESTIPSSIFVCETGGMVVVCAGTSGYNATV
                     DLRYLWMRQKRLQGSHFANTVQCNEMNQLALRGELDPCMSRAFVYNELPDAHLLMHDN
                     KHPHGNMAVLIGAPSFGLGASGKPEVEMVHPVLPKGDVHTTPHPYPMSEPLPSIESAE
                     AITIVDDGSKVRDLMHAGIISCTPDDTIGAVAKIMVSKEIHAVVVMDAQGSAVGVVSQ
                     TDMVLARQGRTPDQARAMLAREVMTPGCATCDADLLLSEAVSMMTGRRMHRLVVTEKD
                     KPVGVISMTDVVRKIIGE"
     gene            complement(5008..6057)
                     /locus_tag="PLB25_01905"
     CDS             complement(5008..6057)
                     /locus_tag="PLB25_01905"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40379.1"
                     /translation="MRQPDLPDPEQLALRIQRGERAAVASGLNLLDNKLAEARVRATR
                     LLALLSSQRRVSDGHLIGVTGPPGTGKSTLVCAMIREWRQVGRTVGVLAVDPSSRPEL
                     GGGALLGDRIRIKSAGYDEGLFIRSLANRNQLGGVAIEVWPMSWLMLACFDIVVVETV
                     GVGQTEIDIAELADTVCYVAQPASGDAIQYLKSGIIEIPDVFAVNKADLGAPAFKTAA
                     EIGRSAPRQDRRKEWDYPVCLVSAAMNTGIKPMLQRIDQHRDFLVRAGLLDKLRNNHH
                     KAWAIRLLKEEFGTFGLELVGGVTLVGEHLGDGQANQFEEYQRLRKRILSRFQSETQF
                     SQLTKKEHSDEQATV"
     gene            complement(6066..8087)
                     /locus_tag="PLB25_01910"
     CDS             complement(6066..8087)
                     /locus_tag="PLB25_01910"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HOE40380.1"
                     /translation="MATTPKKNKDQPWLMRTYSGHSSAKASNALYLTNLAKGQTGLSV
                     AFDLPTQTGYDADNPLASGEVGKVGVPISHIEDMDQLFDQIPLDKMNTSMTINATAPW
                     LLALYIALAQRRGIDPKMLSGTTQNDIIKEYLSRGTYIYPPGPSMRLIADTINYTVLH
                     VPKWNPTNICSYHLQEAGATPVQELAYALCAAMAVLDRVKASGEIGAAAFPQVVERIS
                     FFVNAGIRFVEECCKLRAFGQMWDELALTRYQVEDPKLRRFRYGVQVNSLGLTEAQPE
                     NNVQRIVLEMLSVVLSKGSRARAIQLPAWNEAMGLPRPWDQQWALRIQQVMALETDLL
                     EYGDILDGSPVIAAKVAELIEGAKKEIANVEAQGGIIAAIESGYIKRELVGSHMSRLR
                     AIESGDLKIIGVNCFQETAQSPLTQGSDGGIMKADPEAEREQIERLQAHRSKRDNIEV
                     RAALQGLTDAANSGENIMPSSIRCALAGVTTGEWGDTLRAVFGEFRPPTGIDIAIDSR
                     VVLGKTDQVTDLRSRVADTGNALGRPLKLLVGKPGLDGHSNGAEQVAVKARDVGFEVV
                     YDGIRLTPQQIAQAAQEEGVHMVGLSILSGSHMQLVESVLQEMRLRDLGQVPVIAGGI
                     IPPEDAEKLKSLGVRAIYTPKDFSLNAIMADMVDVVRECNGLVRLGLLK"
     gene            complement(8090..9271)
                     /locus_tag="PLB25_01915"
     CDS             complement(8090..9271)
                     /locus_tag="PLB25_01915"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HOE40381.1"
                     /translation="MSQAVRFQAQVELAEKVKVDGPRVRFPHYGRYLEEFVPGQVFVH
                     PRGFTFLSAQMEAFARTYLQCNPLYLNDQYATACGFAGLLASPQMVFNVVLSLGVQND
                     SEKAMANLGYYDAQYLRPVYAGDTVRSLTKVIDRKERGVGKPGIVTIRTLGLNQNDEV
                     VLQYQRKIMVDGQGSRPPTTPLPSAATMPTFPWVEQAEVLLPNFPAQLPSGLTGPNSW
                     FEDFVVGDVIVHANGRTITDEHLSLTYAVGNTHPLHFDQVFSSGLSGKMSGDPIVYGG
                     LVFAWLEGLASRDISENSLWEIGFTEGYHTQPSIAGDTVAAMSRVLAVADAPGGLGAD
                     FGIVNFQLIGVKNISASKAYAKHGADLFIKESDKKAMGKEKIDEKIFEIERQLLIRKR
                     K"
     gene            complement(9319..10998)
                     /locus_tag="PLB25_01920"
     CDS             complement(9319..10998)
                     /locus_tag="PLB25_01920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOE40382.1"
                     /translation="MSEVNNRLQEASQHLKVADALIKKSLLWVKEQCLGGKGVSGEKL
                     DGHQMASFDLAWCAAEATTARFAVEYAGKVIADSQASGASLCLEEQMTLVFCAEALQN
                     IRNRFAARPQSFGLGEGDIALLDASVLTRFCRTQLDAGNLEALGRQVIAMDGASGVYL
                     LGDDAMMMQDSFRKLATHVVMPLAEEIHRHDKIIPKEILVPLTEMGCFGLSIPERYGG
                     IQSDDHEDNMGMIVVTEELSRGSLGGAGSLITRPEILSRALQKGGTEEQRQKWLPQVA
                     MGHPLCAIAVTEPDTGSDVAAIRLKATKCEGGWHLDGVKTWCTMAGKAGLLLVLARSN
                     PDRAAAHKGLSMFLVEKDSYEGHDFEFRQPQGGVLTGRAISTIGYRGMHSYELFFDHV
                     FVPDENLVGGPEGEGKGFYSTMAGFAGGRIQTAARAIGVMQAAFERAVLYATDRKVFG
                     KAIGEYQLTLVKLARMATLLTVSRQFTYAVGRLMDQGEGQMEASMVKLFTCRTAEWLT
                     REALQIHGGMGYAEETAVSRFFVDARVLSIFEGAEETLALKVIARSLIDSV"
     gene            complement(11032..12474)
                     /locus_tag="PLB25_01925"
     CDS             complement(11032..12474)
                     /locus_tag="PLB25_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40383.1"
                     /translation="MKLTLTHDEMSAYCAELHDANAVFKSDYPADSFERQPVHTVYGG
                     ANLFKAGFAPKLGEVALKTLETYAPSFSVFAKVLGLPRAEMLPSNPFELGSLTRALEA
                     NPEQVRETKPAAWLAFTVYNRVVKKLQAEPIEDNRIDFEDGYGNRPDAEEDAHAVAAA
                     DEVAKGMADNVLSPFIGIRVKTFSDECKVRSIRTLDIFLTRLAEKTGSKLPKNFIVTL
                     PKVTIPTQVSTFVKILENLETRLGFAKDSLKMEIMIETTQSIINHKGENTVPLLVAAA
                     GGRCRSAIFGTYDYTASCNITAAHQTHTHPACDFARHVLQVSLAGTGITISDGATTIM
                     PIGPHKAPKDGVLSAQQMDENRSVVHGAWKLHYDNIMHSLRHGYYQGWDLNPGQLPVR
                     YAAMDAFFLSGLQDASARLNAFLNKAAQATLVGNTFDDAATGQGLLNFFLRGMACGAI
                     TEQEVLATGITLDELRSRSFVRIVSNRTGK"
     gene            complement(13150..14478)
                     /locus_tag="PLB25_01930"
     CDS             complement(13150..14478)
                     /locus_tag="PLB25_01930"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HOE40384.1"
                     /translation="MGIQIIKMPDIGEGIAEVELVAWHVQVGDTVAEDQILADVMTDK
                     ATVEIPSPVSGIVLALGGAVGQVMAVGSEVIRIEVNGAGNLKSGPGAVPAPASADPPT
                     ATLQTTPAANIAPTVQTAADDGNGKPASVPVAVPATDTQPASPARGTDRPIAAPTVRR
                     RAWELGIDLSQVQASGTAGRITQADLARHATSQYDYDSYQRKQDKGYRPLEAQETIPV
                     IGLRRKIAQKMQEAKRRIPHFTYVEEIDVTELEALRTTLNNQHGQTRGRLTLLPLLIR
                     AMVLAVREHPEVNARYDDDAGVLTRFAALHLGVATQTDGGLMVPVIRHAEALDLWDCA
                     REILRLAEAARSGKASREDLTGSTLTLTSLGALGGIVSTPVINHPEVAIVGTNRIVER
                     PIVIGGTVVVRKMMNLSSSFDHRVVDGLHAAKFIQRLRSLLECPGTLFAG"
     gene            complement(14482..>15108)
                     /locus_tag="PLB25_01935"
     CDS             complement(14482..>15108)
                     /locus_tag="PLB25_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOE40385.1"
                     /translation="SQSPEAMFTQVCGLRTVMPSNPYDAKGLLIASIENDDPVIFLEP
                     KRLYNGPFDGRHDLPVVPWSKHPLGMVPDGYYTVPLDSASVYKQGQALTVLAYGTMVY
                     VAEAAALESGVDAEIIDLRSLWPLDLDTLVTSVKKTGHCVIVHEATRTNGLGAELSAL
                     IQEHCFYHLEAPIERVAGWDTPYPHAQEWAYFPGPARVAAAFKRVLEA"
BASE COUNT         3226 a         4360 c         4239 g         3283 t
ORIGIN      
        1 gatcgacaac accgccgcaa agaactacat cgcgcgttac ttcgagcgct atcctggcgt
       61 caaaatctac atggaccata cccggcagtt ggccaaagcg cagggctatg tggaaaccgt
      121 gtttggccgc aggttgtatc tgcccgagat caactcgccc aacgggccgc gccgcgccgg
      181 ggccgagcgg gctgccatca atgccccgat gcagggcaca gccgccgatt tgatcaagct
      241 cagcatggtc aaggtgcagc aggttatcga tgcacaacac ctggcgacca aaatgatcat
      301 gcaggtgcat gacgagctgg tgtttgaggt gccgatgtcg gaagtggatt gggtcaaaca
      361 tgaagtcccc aaactgatgg cgggcgttgc gcacctgaaa gtgccgctac tggctgaggt
      421 tggtatgggc gtgaactggg acaaggcaca ttgagggtgg catgacttca actggcaact
      481 tgcacggaca gtttggtccg aggccgacca ccacacttca tttgcggagc atcttcagtc
      541 atgtcactgt gctgattcac gtcaatggct taccgttcgg gcacagcgct atctatttca
      601 aggagtgatc gggtggaaaa catgggagct ttggcagttg cgggctgcat catcagcgca
      661 gcggcagtgt ttgttgttat gcagttccgg cgttatgaag aacagcaaga tgccagcaag
      721 aagatactca aggccacgac cgacttcgag tccaccaaga ccaaactgct gggctatacc
      781 aaatttaacg acagcctgtc ggctgcgaag ccgcatctgg tgcaacttgc caagagtctg
      841 gcggctaatg tggtgcgcga gtatgtgcat ctggagcgct tcggcaaaga caaacacaag
      901 cttaaatctg acgtgatcgt tattggaaaa tacacggtgg agttttgttt tgccattgac
      961 ctaaagcctg accgcttcga gatagagtcg gaaggtgccg gcatttccat caaatgcggt
     1021 caacctacgc tgatctcgcc gccctcaacc aaatcggcat ctcacgaagt ctctgtgcca
     1081 gatgtgctag ctgacgaacg ggccaccttt attgaggtaa accagaaatt taacgagctt
     1141 gcgcaacgtt acggcctggc agttgccaga gaagatgcag tgcgtgcact ttgcaaggcc
     1201 aaactggtcg acgcactgcg cgacttcctg gccaaagagc ctggggtcag acagatacca
     1261 accattgtgg tggtgtttcg gtaggcccac gcacggcctg tgctgacatc gagttcccgg
     1321 tgcagatcag gtttctggga tttgagttta tcgatagcgc tcgcagttca gcggtttgca
     1381 cctgagccag gtcaacgaca gttgacctta ccctatcgac atcgagatag cggcttctgc
     1441 ctgatgctgc cagcctgcga aaggctggct gacatctcgc agagcctgtg ggagcgagac
     1501 tactttgcag cagcttcgcg ttttttgcgc gacgaaacca tcagcgtggc ctcaccatca
     1561 atgaccacct tgtcacccac ggtgcagacg gtatcgacga ccacgcgggc tttctccgca
     1621 atgacttcct tgaccgtgac cttggcatga accgtttcgc ccgggcgcac cggcgcacga
     1681 aagcgcagcg actgactcat gtaaatggtt ccgggaccgg gcaggcggtt gcccagaacg
     1741 gctgaaatga ggctggctga cagcatgccg tgcgcaatgc gcccgccaaa catggtggtt
     1801 cgaccaaact cttcgtcgat gtgaactgca ttggtgtcgg ttgacactgc agaaaaaagc
     1861 aaaatgtctg cgtcggtaat cgttttggcg atttctgcgc tcatgccaac cgtgatgtct
     1921 tcgacgtcgt aaccgttttg tggattcata taagtccttt tggtgatgtt aagcgcgggt
     1981 tattccgtgc cgttgggaaa tgacgcaagc gaagcctgcg tcacgctgat ttttcgtggc
     2041 ttcaagttgc tggctaaata cattgcacag aacctcggtg gcaagcgcga aagctccttt
     2101 gtcaagagga tcgaactcag ccaatcgcag ggcagcagaa tgacagcgca tcttggttgg
     2161 tcaagacatt gtgatgatga ctttgcccgt cgatttgcgg gtcagcacct gattcaaggc
     2221 atcgacggcc tgctccagcg gatagatcgc cgagacgtgc ggcttgatgg cacccgctag
     2281 ataccattga atcagagtct ggaaactctc ggtcatgact gttggattca attgggcgta
     2341 agccggccag ttgacgccga tcacatcgac gtttttcacc agcaaatggt tggccggaat
     2401 ctgcggcacg acaccaccgg caaagccaat gatcaggatt cgcccttcaa aggcgatact
     2461 gcgcagtgac gcagcaaaca tgtcgccacc gatcggttcg tagatgacat cggcaccccg
     2521 gccaccggtt aactccttga tccgcatgcg caaatcctcc tggctggcat caagcagatg
     2581 atccgcgccg tgtgctttgg ccacagccag cttgtctggg ccattggccg tggcaatgac
     2641 agttgctccg agttgcttgc cgatcgccac agccgttaac ccgacaccac ctgaggcacc
     2701 atgcactacc agtgtctcac cagagcgcaa ccgtgcccga tgccacaaag cgacatgcga
     2761 ggtgccaaaa accacaggaa aggccgccgc gtgctcccag gacatccccg ctggcatcgg
     2821 cacacagcgg ttcatgtcgg ctgcgacctt ttccgcgtag ccaccgtgcg cagacatggc
     2881 gatcacccga tctccaatgg caaactttga aacgccagcg ccaagttcca gcacctcacc
     2941 agcaacttca aagccaggac tgaagggcaa ttgcggctgc atttgatact ggccacgggt
     3001 gatcagacta tcggcaaaat tgacgccaca agcccgcaca cggataagca cctggtcagc
     3061 acggggtcgc gggtcgtcga cctgcatcag acgcagggct gaaggaccgg tgagttcatg
     3121 gcagacgaca gcacgcatgg caaaaaagtt tgaccgaatc aaagaaaata ctttgctctg
     3181 actgaggcca gagcaaagtg ctttttaaaa ctccctctat ggacggtgag ggagcagcgc
     3241 actgcgtcgc aatgcgctgg attgggcggc gattactcgc cgatgatctt gcgtaccacg
     3301 tcggtcatgg agatcacacc gactggcttg tccttctcgg taacgaccag gcggtgcatg
     3361 cgccgacccg tcatcatgct aaccgcttcg ctcagcagca ggtcggcatc gcaagtggcg
     3421 caaccggggg tcatgacctc gcgcgccagc atcgcgcgcg cctgatcggg ggtgcgaccc
     3481 tggcgcgcca gaaccatatc ggtctgcgac acgacaccca cggcagagcc ctgagcgtcc
     3541 atgaccacca ccgcatggat ttccttgctc accatgatct tggctacagc gccaatcgta
     3601 tcgtctggcg tgcaggagat gatgccggca tgcatcagat cgcgcacctt gctgccatca
     3661 tcgacgatgg tgattgcctc agccgattca atgctgggca gcggctcgga catcgggtac
     3721 ggatgtggcg tggtatgcac gtcacccttg ggcagcacgg gatgcaccat ttcaacctcc
     3781 ggttttccac tggcacccag gccgaagctg ggggcaccga tcagcacggc catgttgccg
     3841 tgcgggtgct tgttgtcatg catcaacaga tgggcatctg gcaattcgtt atagacaaag
     3901 gcacgcgaca tgcaggggtc aagttcgccc cgaagcgcca attggttcat ctcgttgcac
     3961 tgcacggtat tggcgaaatg cgagccttgc agacgctttt ggcgcatcca caggtagcgc
     4021 agatcgactg tggcgttgta gccggaagtg ccagcgcaga ccaccaccat gccaccggtt
     4081 tcgcagacaa aaatcgaact cgggatggtg ctttcaccgg gatgctcaaa gaccagattt
     4141 ggggcgcgct tttcacccag cacttcccag atttttgcgc caaacgagcg cacacctttg
     4201 agccatttac cgtacccagc gttgtctttc cagtgtggca acatacccca gtggtcgaaa
     4261 tcattgcggt tgatgcagcc tttggcacca agcttcatgc agtaatcaaa cttatcctgg
     4321 cctgacacca tggccaccga ggtggcaccg accgccttgg caatctgaat tgccatcgaa
     4381 cccagaccac cagcaccgcc ccagatcaag gccacgtcgc cctttttgat ggagctcgat
     4441 tcccagccat gcaacatccg ccaggcggtt gccgccacca gcacatagga ggcggcttct
     4501 tcccagttca tgtgtttagg cttgggcatg cactgttgtc cctgcacccg ggtgaactgg
     4561 gcaaaactgc caaagttggt ctcatagccc caaatcttga acgatggcga gtacattgga
     4621 tcgccaccgc ccttgaccca ttcacaattg cggttgaaag tgccacagtg catcacgact
     4681 tcatcgccga ctttgacgtt ggttacgtcc ttgcctactt tgtagacgat acccgaggca
     4741 tcgctaccgc caatatgaaa cggctcgggt tcgccatttt tattgcgggc tgcaatcacg
     4801 ttgaccggaa tgccgagcgc ggcccagacg ttgttgtagt tgatgccagc cgccatcaca
     4861 tacaccagca cttcgtcgtc agcaatgctc ggggtgtcca ccacttcctt ttgaaacgcc
     4921 tccataggct taccaaagcg ctcaggccgg atcagccatg cgtgcatttt ctccgggacc
     4981 aggcccagag ggggttgttc gcctacttca tacagttgct tgctcatcag aatgctcctt
     5041 tttggttaat tgactgaact gtgtttccga ctggaatctg gacagaatac gcttgcgaag
     5101 ccgctggtat tcctcaaatt ggttggcctg accatcgcca aggtgctcac cgacaagtgt
     5161 cactccgccg accagttcca ggccgaaagt gccgaattct tctttgagca agcgaatggc
     5221 ccaagccttg tgatggttgt tacgcagttt gtccagcaac ccggcgcgga caagaaaatc
     5281 ccggtgctgg tcaatgcgtt gcaacattgg cttgatgccg gtgttcatcg cggcacttac
     5341 cagacagact gggtaatccc attccttgcg acgatcctgc cggggtgcac tgcgaccaat
     5401 ctcggcggcg gtcttgaagg caggcgcgcc tagatcggcc ttgttgaccg cgaaaacgtc
     5461 ggggatctcg atgattcccg acttcaggta ctgaatagcg tcgcccgagg ctggttgcgc
     5521 tacatagcac acagtgtcgg caagctcggc tatatcaatt tcagtttgac caacacccac
     5581 ggtttcgacc acgactatgt caaaacaggc cagcatcagc caactcattg gccaaacttc
     5641 aatggcaaca ccgcccaact gattgcgatt ggcaagcgag cgaatgaaca gtccctcatc
     5701 gtagccagcg cttttgatgc gaatacggtc cccaagcagg gcacccccac ccaattcagg
     5761 acggctcgac ggatcaactg ccagcacacc aacggttctg ccgacttgcc gccattcccg
     5821 gatcatggcg caaaccagcg ttgacttccc ggtgccgggc gggccagtaa cgccgatcag
     5881 atgaccgtca ctcactcgtc tttggcttga aagcagcgcc agaagcctgg tggcgcggac
     5941 acgtgcctcg gccagtttgt tgtcaagcag attcaaacca ctggcaactg cagcccgctc
     6001 accgcgttgt atcctcaatg ccaattgctc gggatcaggt aggtccggtt ggcgcattgt
     6061 tgtcgttact tcaataatcc aagacgcacc agtccgttgc attcccggac cacgtcgacc
     6121 atatcggcca tgatggcatt caggctgaaa tccttgggcg tgtagatggc ccgcacaccc
     6181 aatgacttga gcttttctgc atcttctggc ggaatgatgc caccagcgat taccggcacc
     6241 tgaccaagat cgcgcagccg catttcctgc aataccgatt cgaccaattg catgtggctg
     6301 cctgagagaa tcgacaggcc aaccatatgc acaccttcct cttgtgctgc ttgcgcaatt
     6361 tgctgcgggg tcaaccggat gccgtcatag acgacttcaa agccgacatc gcgggctttg
     6421 accgcaacct gttcagcgcc attggagtgc ccgtcaagac ccggtttgcc aaccaacaat
     6481 ttcaggggcc gacccagcgc gttgccagtg tctgccactc tgctcctgag atcggtcacc
     6541 tggtccgttt tgcccagcac aacacgactg tcgatggcaa tgtcaatacc tgtgggtggc
     6601 cggaactcac cgaataccgc gcgcagggtg tcgccccatt cgccagtggt gactcctgcg
     6661 agggcgcagc gaatcgaact gggcatgatg ttctcgccgc tgttggcggc atcggtgagg
     6721 ccctgcaagg cggcacgaac ttcgatgttg tcgcgtttgc tgcgatgcgc ctgcaggcgt
     6781 tcgatttgtt cacgctctgc ctctggatcg gccttcataa tgccgccgtc gctgccctgg
     6841 gtgagcggcg attgggcggt ttcctggaag cagttgacac cgatgatctt caggtcgcca
     6901 gactcaatgg cacgcagacg gctcatgtgg ctgccgacca gttcacgctt gatgtagccg
     6961 gactcaatgg cagcaatgat tccgccctgg gcctcgacat ttgcgatctc ctttttggca
     7021 ccctcaatca gttcggccac cttggcagca atgacggggg agccgtccag aatgtcacca
     7081 tactccagta aatcggtttc tagcgccatg acttgctgaa tccgcagggc ccattgctga
     7141 tcccacgggc gcggcagccc catggcctcg ttccaggccg ggagctggat agcgcgggct
     7201 cttgaacctt tggagagcac taccgacaac atttccaaaa cgatgcgctg cacattgttt
     7261 tctggttgcg cctcggtcag gcccaatgag ttgacctgaa ccccgtagcg gaaccggcgc
     7321 agctttggat cttcaacctg gtagcgggtc agggccaatt catcccacat ttggccaaaa
     7381 gccctgagct tgcaacattc ttcaacgaag cgtatgccgg cgtttacaaa gaacgagatg
     7441 cgctcgacca cctgcgggaa ggcagcggct ccgatttcac cggatgcctt gacccggtcg
     7501 agtaccgcca tggcggcgca cagggcataa gccagttcct gaaccggggt ggcaccggcc
     7561 tcttgcaaat ggtagctgca aatgttggtc ggattccact tcggcacatg caagacggtg
     7621 taattaatcg tgtcagcgat caagcgcatt gaagggccgg gcgggtagat ataggtgccg
     7681 cgcgacaggt attccttgat gatgtcgttc tgcgtggtgc cgctcaacat ttttgggtca
     7741 atgccgcgtc gctgcgccaa tgcgatgtac agcgccagca accagggtgc tgtggcattg
     7801 atcgtcatcg aggtgttcat cttgtcgagc ggaatttggt caaacaactg atccatgtcc
     7861 tcgatgtgcg agatgggcac tccgaccttg ccaacttcgc cgctggccag aggattgtca
     7921 gcatcgtagc cggtctgcgt tggcagatcg aaagcgaccg acagaccagt ctgacccttg
     7981 gccagattgg tgaggtaaag cgcgttggat gccttggctg aactgtggcc gctgtaggtc
     8041 cgcatcaacc agggctggtc cttatttttt ttcggggtgg ttgccatgac tatttcctct
     8101 tcctgatcaa aagttgtctc tcgatctcaa aaatcttctc atcaattttt tccttgccca
     8161 tggccttctt gtcgctttcc ttgatgaaca ggtccgcacc atgtttggca taggcttttg
     8221 aggcgctgat gttcttgaca ccaatcaact ggaaattaac gatgccgaaa tcggcaccca
     8281 gcccgccggg tgcgtctgca accgctaaca cacgcgacat cgcggcaacc gtgtcaccgg
     8341 cgatggatgg ttgagtgtgg taaccctcgg tgaaaccgat ttcccacagg ctgttttcgc
     8401 tgatgtcacg gctggccagt ccttccagcc aggcgaaaac cagtccgcca taaacaatcg
     8461 gatcgccact catcttgcct gacaggccgg aactgaaaac ctggtcaaaa tgcagcggat
     8521 gggtgttgcc cactgcatag gtcagcgaaa ggtgctcgtc ggtgatggtt ctgccgttgg
     8581 catgaacgat gacatcaccc accacaaaat cctcgaacca tgaattcgga ccggtcaaac
     8641 ccgatggcaa ttgcgcgggg aagttcggca gcaacacttc ggcttgctcg acccatggaa
     8701 aagtcggcat cgtcgcagcg cttggcaatg gggtggttgg cggtcggcta ccttgcccgt
     8761 cgaccatgat ttttctctgg tactgcagca ccacttcgtc gttctggtta agccccaagg
     8821 tacggatggt gacgatgcca ggcttgccga caccacgctc cttgcgatct atcaccttgg
     8881 tcagggaacg aacggtatcg ccagcataga ccggtcgcaa atactgggca tcgtagtagc
     8941 cgaggttggc catcgccttc tcggagtcgt tctgtacgcc tagcgagaga acgacgttga
     9001 aaaccatttg cggtgaagcc agcagaccgg caaagccaca ggccgtggcg tactggtcat
     9061 tcaggtaaag cggattgcat tgcaagtaag tgcgggcaaa agcctccatt tgggccgaaa
     9121 gaaaggtgaa accacggggg tgaacaaaca cttgcccggg cacaaactct tccagatagc
     9181 gaccgtaatg cggaaagcga accctgggcc cgtcaacctt caccttttcg gccagttcaa
     9241 cctgtgcctg aaaacgaact gcctgtgaca tgggatgtgc tcctttggac ataacgttgt
     9301 aacggcatca agccaaggct agacggaatc tatgagagaa cgtgcgatga ctttcaaagc
     9361 cagcgtttct tctgctcctt cgaaaatcga caacacacgt gcatcgacaa agaagcggct
     9421 gactgctgtc tcttcggcgt aacccatgcc gccatgaatc tgcagcgctt cgcgggtcag
     9481 ccattccgca gttcggcagg tgaacagttt caccatgctg gcttccatct ggccttcacc
     9541 ctgatccatc aaacggccta ccgcataggt gaattggcgt gacactgtca gcagggtcgc
     9601 catgcgggcc agtttgacca gcgtgagctg gtattcgcca atcgccttgc caaaaacctt
     9661 gcggtcagtg gcatacaaga ctgcccgctc aaacgccgct tgcataaccc caatggcgcg
     9721 tgcggcggtt tgaatccgcc caccggcaaa gccggccatc gtcgagtaaa agcccttgcc
     9781 ttcgccctcc ggtccgccga ccaagttctc atcgggcaca aacacatggt cgaaaaacaa
     9841 ctcataagaa tgcatgccgc ggtagcctat ggtcgagatg gcacgaccgg tcaatacacc
     9901 accctgaggc tgacggaatt caaaatcgtg accctcgtag gagtctttct cgaccaagaa
     9961 catgctcagg cctttgtgcg cggctgcacg gtccgggttg gagcgtgcca acaccagcag
    10021 caggccagcc ttgccagcca tggtgcacca agtcttgaca ccgtccaggt gccagccgcc
    10081 ctcacacttg gtcgccttga gtcggatggc tgcaacatcc gatccggtat cgggttcggt
    10141 gacggcaatg gcgcacaagg gatgccccat ggccacttgc ggcagccact tttggcgttg
    10201 ctcctcggtg ccaccctttt gcagggcacg gctgaggatc tcggggcgag taatcagact
    10261 acccgcaccg ccaagtgacc cgcgagacaa ctcttcggtg accacgatca tgcccatgtt
    10321 gtcttcatga tcgtcacttt ggatgccgcc gtagcgctct ggtattgaca ggccgaagca
    10381 gcccatttcg gtgagtggca ccaaaatttc ttttggaatg atcttgtcgt gccggtgtat
    10441 ctcttcggcc agcggcatta ccacatgggt ggccagtttg cggaaagaat cctgcatcat
    10501 catggcatcg tcgccaagca ggtagacacc gctggcacca tccatggcaa tgacttgccg
    10561 acccagcgct tccagattgc cagcatcaag ctgtgtgcgg cagaagcggg tcaggacgct
    10621 ggcatccagc aacgcaatgt caccttcacc cagaccaaaa ctctgcggtc tggccgcgaa
    10681 ccggttgcgt atgttttgca gcgcttcggc gcaaaaaacc agggtcatct gctcttccag
    10741 gcagaggcta gcgccgctgg cctggctgtc ggcaatgacc ttgcccgcat actcgacggc
    10801 aaaacgcgcc gtggttgctt cggcggcaca ccaggccaga tcgaaactgg ccatttgatg
    10861 accatcaagc ttctcgcccg acacgccctt gcctccgagg cattgctcct ttacccagag
    10921 cagagatttc ttgatcagtg catcggccac tttgagatgc tgcgacgctt cctgcagcct
    10981 gttgttgact tcactcatgg tttattgctt tcttgatggg ctgatcagcc attatttgcc
    11041 agtgcgattg ctgacaatgc gaacaaagga acgagagcgc agttcgtcaa gcgtgatgcc
    11101 ggtggccagc acttcctgtt cggtgatggc tccgcaggcc atgccgcgca agaagaagtt
    11161 caacagccct tggccggtcg cggcatcgtc gaatgtattg cccaccagcg tggcttgggc
    11221 ggccttgttc agaaatgcgt tcagccgggc cgatgcgtct tgcagcccac tgaggaaaaa
    11281 agcatccatc gcagcatagc gcactggcaa ctggcccgga ttcaagtccc agccttggta
    11341 gtaaccgtgg cgcagcgagt gcatgatgtt gtcgtagtgc agtttccagg caccatgcac
    11401 caccgagcgg ttttcgtcca tctgctgggc cgacagcacg ccgtccttgg gtgccttgtg
    11461 cggcccgatc ggcatgatgg tcgtggcacc gtcgctgatg gtgatgccgg tgccagccag
    11521 actgacttgc agcacatggc gggcaaaatc gcaggccggg tgcgtgtggg tctgatgcgc
    11581 agcggtgatg ttgcaggatg cggtgtagtc gtaagtgccg aaaatggccg aacggcagcg
    11641 gccaccggca gcagccacca gcagcggcac cgtgttttcg cctttgtgat tgatgatcga
    11701 ctgggtggtc tcgatcatga tttccatttt cagcgaatct ttggcaaaac ccaggcgggt
    11761 ctcaagattt tcgaggatct tgacaaaggt ggaaacttgt gtcggtatgg tgaccttggg
    11821 caatgtcacg ataaagttct ttggcagctt gctgccagtt ttctcggcca gccgcgtcag
    11881 gaaaatatcg agcgtacgaa tcgaacgcac cttgcattcg tctgagaagg ttttgacccg
    11941 gatgccgata aagggcgaca gaacgttgtc agccatgcct ttggcgactt catcggcggc
    12001 tgctacggca tgggcatcct cttcggcatc tggacggttg ccatagccat cttcaaaatc
    12061 gatccggttg tcttcaatcg gctcggcctg aagcttcttg acgacacggt tatagacggt
    12121 gaaagccagc caggccgctg gcttggtctc gcgcacttgc tcaggattgg cttccagcgc
    12181 gcgcgtcagg ctacccaact cgaatggatt ggacggcagc atttcagctc gcggcaagcc
    12241 caaaaccttg gcaaaaacgc taaagctggg tgcataagtc tccagggtct ttaaggctac
    12301 ctcgcccagt ttgggtgcaa aacctgcttt gaaaaggttg gcgccaccat acacggtgtg
    12361 cactggttgc cgctcaaatg aatcggccgg atagtcggac ttgaatactg cattggcatc
    12421 atgcagttcg gcgcagtaag cgctcatttc gtcgtgggtc agcgtgagct tcatgtgttg
    12481 tctccgtcat tgttacaaaa aacacatcca tcattggttg caaattcgcc ctcaaccggg
    12541 gtgcggattc aaatgccaga cgagaaacaa caggaccact gacccatgtt gcagcaagac
    12601 gagcagtcac gtgcacaagg tcctccggcc aaaaacgatc gctgaaagcg acaaatttgc
    12661 tgacagaggg ctgctatgaa cctgaacacc accaaaaccc agccgaaccg aaataagcag
    12721 cgtgattatg cgtcaaatta tgctgctttg cagcaattgg ttcacagttc cgttatatcg
    12781 aggcgctgca gaatctggtt taagtgattg atttgaaagg aaaaaaaatt tttccaagga
    12841 aaacttgtgt ttttgaagat ctgcgatatt tcagaatata agtgccgttg aatatttgtg
    12901 tcggattggg ctcaactggc gttggcgatt caatggcagg ctcgctgttt tgcctgtgcg
    12961 gacaactgag acaccgtgcc cttgcctcgg ggcagatcaa ccggcttgga ggccgactca
    13021 agcaagaagg ccacgaactg ccgggcaaac tcgcgaatcg aacatgcgta ccgacgcact
    13081 caatggctaa caacctcagt gcagatgcgg gcagtcaatc tcggcctgcg ccatgaaaat
    13141 tacccgtgtt caaccggcaa aaagtgtccc tgggcattcc agcaggctgc gcaggcgttg
    13201 aatgaacttt gcggcgtgca ggccgtccac cacccggtgg tcgaacgacg acgacaggtt
    13261 catcatctta cgcaccacca cggtgccgcc gatgacaatg gggcgttcaa caattcggtt
    13321 ggtgccgaca attgccacct cggggtggtt gatgaccggc gtgctgacaa tgccgcccaa
    13381 agcacccagg ctggttagcg tcagggtcga gccggtcagg tcttcccggc tggctttgcc
    13441 gcttcgcgca gcttcggcca ggcgcagaat ttccctggcg cagtcccaca ggtccagcgc
    13501 ttcggcgtgt cgaatcaccg gcaccatcag gccgccatcg gtttgtgttg ccacgccgag
    13561 atgcagggcg gcaaaacggg tcagaacgcc tgcatcgtcg tcatagcggg cgttgacctc
    13621 ggggtgctcg cgcaccgcca gcaccatcgc ccggatcaac agcggcaaca aggttaggcg
    13681 gccacgggtt tggccgtgtt ggttgttaag cgtggttcgc agcgcttcga gttcggtcac
    13741 atcgatttct tccacataag tgaaatgcgg aatgcggcgc ttggcttcct gcatcttctg
    13801 ggcgatcttg cggcgcaggc cgatgaccgg aatcgtttct tgtgcctcta acggcctgta
    13861 gcccttgtcc tgcttgcgct ggtagctatc ataatcatat tgactggtgg catggcgggc
    13921 gaggtcggct tgggtgattc gacctgcggt tccgctggct tgcacctgcg acaggtcgat
    13981 accgagttcc caggcgcgac ggcgcaccgt cggcgcggca atcggcctgt cggtgccgcg
    14041 agcgggcgac gcaggttgcg tatcggtggc aggcacagca acggggacac ttgccggttt
    14101 gccgttgcca tcatctgcag ccgtttgcac agtgggtgcg atgttggctg cgggcgttgt
    14161 ttgcagggtg gctgtcggcg ggtccgcact ggcgggtgct ggcaccgcac ctggcccgct
    14221 cttgagattg cccgcgccat tgacctcgat gcggatcacc tctgagccta ctgccatgac
    14281 ctgtccgaca gcgcccccga gtgccagcac gatgccgctt accggcgacg gaatttccac
    14341 cgtggccttg tcggtcatga cgtcggccaa aatctggtct tcggccaccg tgtcgcccac
    14401 ctgcacatgc caggcgacca gttcaacttc ggcaatgccc tcgccaatgt cgggcatctt
    14461 gatgatttgt atgcccatga ttcacgcctc cagcacacgt ttgaaggcgg cggccacgcg
    14521 ggccgggcca ggaaaatacg cccattcctg ggcatgaggg tagggcgtgt cccagccagc
    14581 tacgcgctca atcggcgctt ccaggtggta gaagcaatgc tcttgaatca gtgccgagag
    14641 ttcagcgccc aggccgttgg tgcgggtggc ttcatgcacg atgacgcagt ggccggtctt
    14701 tttgaccgag gtcaccagcg tgtcaaggtc cagcggccaa aggctgcgca ggtcgatgat
    14761 ctcggcatcc acgccgctct caagggctgc tgcttctgct acgtaaacca tagtgccgta
    14821 ggcaagcaca gtaagggctt gaccctgttt atatactgaa gcggagtcga gcggcacggt
    14881 gtaatagccg tctggcacca tacccagcgg atgttttgac caaggcacca ccggcaaatc
    14941 atggcgaccg tcaaacggtc cgttgtacag gcgcttgggc tccagaaata tgaccgggtc
    15001 gtcgttttca atcgatgcga tcaacagccc cttggcatca taggggttgg acggcatcac
    15061 ggtgcgcagc ccgcagacct gggtgaacat ggcctcgggg ctctggct
//
LOCUS       DAOSLL010000026        15004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4114_length_15004_cov_5.061743, whole genome shotgun sequence.
ACCESSION   DAOSLL010000026 DAOSLL010000000
VERSION     DAOSLL010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 15004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15004
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4114_length_15004_cov_5.061743"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..503
                     /locus_tag="PLB25_01940"
     CDS             147..503
                     /locus_tag="PLB25_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40386.1"
                     /translation="MSIYDTVDLSEIMLGCYQRYLAFLASLDDTSAGERDWQRLCQPR
                     VQVGSEQKIKGLNSFNRSELRVHKVLQHGEFNIYGWRRADLAALVPMTQSAPSSQLAR
                     LKSVGLIKNVCDPHLW"
     gene            741..2069
                     /locus_tag="PLB25_01945"
     CDS             741..2069
                     /locus_tag="PLB25_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014725816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HOE40387.1"
                     /translation="MSGFTRLAGSYRLHPGQTPGISGHRPVLGQGLLTRLLRGQSGET
                     VERTLSALFTLCSHAHRRTARLVLNAARWPGQAALPEPPKLLLTLETARDHLRSIALD
                     WPRQLVLLALPDRSDDGSNHLLALMESAQHAIKINSFDWLKACPLALSQTHLAPTWQE
                     AEHALTQLRHWLEVSVLQQPPDDWLAQHCTPDALVQWCRNRSTDLPPAQFLAACHRQA
                     SALIPGARTLDVLDHHPQEQAAKLAELARLIVAQPDFAQFPAWCGQCLETGPWARLRH
                     RRLEPGSAAANRPTDKPSAWVRLASRWLELIELANAPVTLGSPPPEQKAIAAPGIVAN
                     DMALPALLGPGKDRPAALSSGALKLGDGQAIAWCEMARGLLLHWVQLDTQGKVADYRV
                     IAPTEWNFHPQGALSLAVSALPPQDTASACILGAAYDACVECSVISSGSK"
     gene            complement(2300..2719)
                     /locus_tag="PLB25_01950"
     CDS             complement(2300..2719)
                     /locus_tag="PLB25_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOE40388.1"
                     /translation="MGAMLAALGTIWHTMVQRDNEKDLLFAGHQFRVGLNRYHQINQR
                     YPMRLDDLVQDQGKPTIRRHLRKIYIDPMTRQTDWGLVKLSDGQIVGVYSKSSLVPLK
                     KTGFRLWDMNLEGKASYSEWLFMAEGQTVASNGLAGN"
     gene            complement(2766..3161)
                     /locus_tag="PLB25_01955"
     CDS             complement(2766..3161)
                     /locus_tag="PLB25_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE40389.1"
                     /translation="MANGSHPLPAQRGFTLIELVVVLAIVALLLSIATPLYFHRLDKS
                     KETVLRANLALTRDVLDKFYGDTGKYPDRLETLVEKKYLRNLPVDPVTESALTWIIMA
                     PDTPDKGGVFDIHSGAPGNSSDGTPYSDW"
     gene            complement(3167..3661)
                     /locus_tag="PLB25_01960"
     CDS             complement(3167..3661)
                     /locus_tag="PLB25_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOE40390.1"
                     /translation="MTVFNRNRQQRGFTLIELVITVAIVGLLASVLVPMAEVAIQRSK
                     EREFRDALQQIRTAIDAYKQAVDDGRILSDAQKSGYPPDLQTLVQGVDDVKSARKGQK
                     MYFLRRIPRDPFFQDAAAPPEASWGKRSYASPPDAPSEGEDVFDVYSWSTGIGLNGIP
                     YQQW"
     gene            complement(3658..6063)
                     /locus_tag="PLB25_01965"
     CDS             complement(3658..6063)
                     /locus_tag="PLB25_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HOE40391.1"
                     /translation="MKSVHHFEACAARRPAWPGAHLCQLMLRLLVVAWLAGCATDSGR
                     REGLSLIKDGRYEEGLARLEQVTQADPKDAESRSDYVRQREQVVNRLLNSARSELANE
                     RFEATEVIYQRVLAIDPNNQAAKSGQRSALAEQRHLKWLADAQSMAKLGDVVGARSAV
                     GLILLEQPTHVKANQMRYQLEEQWLRENLTGPRLNIKERKPVTLTFRDANLRMVLEAI
                     SMAAGLNIVIDKDVRNDIKVSIFVKDTPVEETLDLILLQNQLEKRILGENAVLIYPLT
                     PAKTKEYQELKVRRFALVNADPKQVQAMIKSILKSKDIFVDERSNAVVVRDTVQVIRL
                     AEKLVAAMDQPEPEVMLEVELLDVDRSRAIDLGIDWPTSVSGTINPMTLAALRNSSSA
                     DVNMSSLGMSVKAKGTDSDTRTLATPRIRVRNKEKAKIMIGDRTPVVSSAAVPGSGVT
                     TSPVYNTTIQYLETGVKLEVEPTIYIDGSVAIKVNLEVSSLGGEVRVDSSLAYKTKTN
                     NATTTLRLKDGETQVLGGLIQGFDSSGQANKIPGLGDVPVFGRLFGISHDEWSGRELV
                     LVMTPHIIRNNQVSEADLLELWSGTESNVKYLPLDLKVAASAGVLTQKGASPASGAAA
                     ARNPSIPFRAPTSQAARPATLASSAVAPLAAEVASATPTLTLSGPIQANPGEKISVAV
                     NVQGGSGLSAVHATLSYDKEALKPISVNEGNLMRRTGIKSNFDARVDEATGSIHADLV
                     TEDGAVASGTGAVAHIEFEVTGKPGRPALIKLSSATAATAGTEAMTLVAPQPLSVNIE
                     PGP"
     gene            complement(6060..>6355)
                     /locus_tag="PLB25_01970"
     CDS             complement(6060..>6355)
                     /locus_tag="PLB25_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40392.1"
                     /translation="PPPPPPPPPPPPPPPPPPPPAPPPPPSAPPLPFVYLGQYIEGDK
                     QLILLTRGVRMISVSVGDVMDKTYKVVSMQGGQLTFLYLPLNVEQSLDTGTNQ"
     assembly_gap    6356..6455
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6456..6735)
                     /locus_tag="PLB25_01975"
     CDS             complement(<6456..6735)
                     /locus_tag="PLB25_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40393.1"
                     /translation="MRPVTRRIWLVLAVLVLLALVWFAPGDEDGLAAPALDKTGRSRK
                     AVAVRSRPTKTTVTPVGAADASHGQLISQGRAPVAENIPDLFAGVSWYL"
     gene            complement(6732..7280)
                     /locus_tag="PLB25_01980"
     CDS             complement(6732..7280)
                     /locus_tag="PLB25_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40394.1"
                     /translation="MLRAQLFFRLRLLGWQGICGLVLVVAALVWAVSIGLLSGARLDQ
                     IDRQLALVTAKLKQGQQTPAAQKPTTATEQLLAFYREFPDHASTPDWLGKIYVLAEQH
                     QLALNVGEYSLTQAQAGRLDMFTITLPVKGTYPQVRKFIRAALLTAPALALEGLDLKR
                     DKVGDGSVEARIVFRLYLEKGS"
     gene            complement(7289..7822)
                     /locus_tag="PLB25_01985"
     CDS             complement(7289..7822)
                     /locus_tag="PLB25_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40395.1"
                     /translation="MRRIDLNFSSRAQPAGAARWVGLLLGIVALVGVMAWDRLYWQPL
                     IATNEKQLRSLQMALAARQPVVPKMDDVQLAAEWGQALAVAGTLNLPWEKLFATLEGQ
                     SVGQVALLSLEPDAVRRELVLTAEGRSLKAMLGFYKMLQQQGDLSAVVLHSHQVNQQD
                     SEKPVRFHITAKWAASS"
     gene            complement(7809..8648)
                     /locus_tag="PLB25_01990"
     CDS             complement(7809..8648)
                     /locus_tag="PLB25_01990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40396.1"
                     /translation="MSLLSPESLCVFVSPTELAAVHWCGLRPQIAAKRICPVSALPGA
                     LWSGAALAFNGVLAEFSTCHRVWVILSSHFTAYQLQPWRDDLHDRQEEDSVARLAFVQ
                     TYGDVAANWQVRLSLDVPGSPRVATAVDADFLAALEQGAMANKARLVSIEPYFAAAVN
                     HWRKQFHRRFTGWVVLHEEGQLCIGLVKRGHWQWLRRLRVGPGWQASLPDLLDNEVML
                     AGIDSVASQALVYSSALAGLSMPANTRWSFRSLTLNARRNFAPATDSRFGPALWTFQN
                     ATN"
     gene            complement(8629..10224)
                     /locus_tag="PLB25_01995"
     CDS             complement(8629..10224)
                     /locus_tag="PLB25_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007739703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOE40397.1"
                     /translation="MVEILQERQDQERFLVRLGLTVRKPVLSMAQLRAMQTAFDVISF
                     KETMSRNCLLLRATDSQLVLVVADPFDAGVQSWAESRTSEPIFVAIASRADIMAWQSV
                     HEESLRAFDTVLPETQEVGPRTEAVEQLSFRGISEDSSRVVRLVNSTIFDALRADASD
                     IHLESNPAGMVIKFRVDGVLGVVGSVASVALTEQTISRIKVMSELDIAERRVPQDGRF
                     RVSIGGREVDLRVSIMPSIHGEDAVLRILDKQSLTDQLKGLQLTSLGFDEKISAILRR
                     LANEPYGMLLVTGPTGSGKTTTLYAAISEINNGTEKIVTIEDPVEYQLSGVLQIPVNE
                     KKGLSFARGLRSILRHDPDKIMVGEIRDAETAQIAIQSALTGHLVFTTVHANNVFDVL
                     GRFMHMGVDPYSFVSALNGVLAQRLLRVNCPQCVADDLPSAALLADSGLSREDVAGFR
                     FRAGQGCGHCRGSGYKGRKAIAEVLHLNDNLRELIVARAPIRQIKEAARQNGTHFLRD
                     SALDLVRLGETTLTEINRVTFVA"
     gene            complement(10323..11513)
                     /locus_tag="PLB25_02000"
     CDS             complement(10323..11513)
                     /locus_tag="PLB25_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOE40398.1"
                     /translation="MRFELRVLRPDARATTLYLDASSVAEATGQARQQGYSILRVKSA
                     KTLANMATSDARVFPLGLFSQELLMLLEAGLSVVESIETLKEKEGKAQTHHVLAQLVT
                     FLCEGQSLSQALQNFPAVFPPLYIATVRASEKTGDLPEALTRYVAYQMQLDVVRKKIV
                     SAAIYPLLLAGVGGLVIVFLLSYVVPRFSRIFEDLGGDIPFASQLLINWGNLVHDHGW
                     TLLIGLLVVTVLAVFWLLQPGTRRWIFDRLATVPAIGERMRLYQLARFYRTVGMLLRG
                     GMSIMPALQMTGDLLQGPLRHRLSESASQIREGLQISSAMHSCGLTTAVSLRMLRVGE
                     RTGRMGEMMERIASYYDAEIARWVDWFVRLFEPLLMVFIGLVIGAIVVMMYFPIFELA
                     GSIQ"
     gene            complement(11543..11968)
                     /gene="gspG"
                     /locus_tag="PLB25_02005"
     CDS             complement(11543..11968)
                     /gene="gspG"
                     /locus_tag="PLB25_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017454507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOE40399.1"
                     /translation="MSIRSTRIVRGFTLLELLVVMVIIGLLVGYVGPMYFKQIGKSEI
                     KTTRAQIDAFGKALEQYRLDTGHFPTTEQGLTSLTEKPPSERKWDGPYLKKKVPADPW
                     GNPYTYKRPGDYGEYDIMSLGRDGALGGAGEDADIGNWE"
     gene            complement(12101..12658)
                     /locus_tag="PLB25_02010"
     CDS             complement(12101..12658)
                     /locus_tag="PLB25_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40400.1"
                     /translation="MLIGGIALWLGAAIPPKTAQALEFAVGLMLMMLGVDVWRRLAKQ
                     QIHVHVHSHDQNLKHVHAHSHLGPQTSECSAHSHGHPDKPPLRALFIGMMHGLAGSAA
                     LIALSLGVESSWFMGLLYIAIFGIGSIVGMALLSAVITVPLGWSARSPGWLHEGLPLG
                     IGGFSCLLGVFVVYRIGVVEGLLFN"
     gene            complement(12820..13101)
                     /locus_tag="PLB25_02015"
     CDS             complement(12820..13101)
                     /locus_tag="PLB25_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HOE40401.1"
                     /translation="MCLGLPGQIVEIISSDMQLAMVDVGGVRRAVNISLLVDDEHPVS
                     ACLGDWVLVHLGFARLRIDENEAAATVALLLEIGKLQSDIDRTRTAQAT"
     gene            complement(13114..>15004)
                     /gene="hypF"
                     /locus_tag="PLB25_02020"
     CDS             complement(13114..>15004)
                     /gene="hypF"
                     /locus_tag="PLB25_02020"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HOE40402.1"
                     /translation="VIILACGSVAALGAFVDDLPAQAPPLACIEAIERESIANRAQES
                     GFRIVASQVGHVHTNITPDAATCPGCLAEIFDPLDRRYRYPFTNCTHCGPRLSIIEAI
                     PYDRGATTMRSFALCAQCANEYADPGNRRFHAQPVACPSCGPHTWLEKAGGGRFDPQS
                     LNAVDDAEAAGTLIKRGFIVAIKGLGGFQLACDAGNEDAVSRLRQLKHRSSRKPLALM
                     ARDLAMVRRYCAISQVEADLLQGPASPIVILDATETQQLAASVAPGVTSLGFMLPNTP
                     LHHLVMQCMERPMVLTSGNVADEPQCTENADALARLSDMVDYFLLHDRGVARRVDDSL
                     VRVVAGLPRLMRRARGYAPAPIALPQGFDDAPAVLAMGGDLNNSFCLLRQGQAIMSHH
                     MGDLTGARCRVDYLQSIESYLQLFEHRPQIVAVDQQPEGLSSKRGRDWGERRGLACEE
                     VQHHHAHIAACLAENGIPRCASPVLGVVLDGLDLDADGSSGGGRFFLADYCQCRALGT
                     VHAGVKSGVGGGRQTMANDDEPMVDALVGETTTRFQKNLTRVIGRMLDDFSGNANRQE
                     PVKTVALSGKLFQSRILLEAVMMQLESVGFRVLTHHQVPFHDGGLALGQAVVAAARSL
                     ALQKN"
BASE COUNT         3262 a         4509 c         4168 g         2965 t
ORIGIN      
        1 ccagttaacc cttttgtagt ggcgcgtttc cagtgcaccc ctatatagat caacacgtta
       61 caaatttagc tggggaactc gggaggcatt acggcaaagt ggaacatcga tacgaacaca
      121 gcacacagga gctggccagt ctgaatatgt caatctacga caccgttgat ctgagcgaga
      181 tcatgctggg ttgctaccaa cgctatttgg cgttcctggc cagcctggat gacaccagtg
      241 cgggtgagcg cgattggcag cgcttgtgcc agcctcgggt gcaagttggt tcagagcaaa
      301 aaatcaaagg cttgaactcc ttcaaccgat ctgagttacg cgtgcacaaa gttttgcagc
      361 atggcgagtt caatatctat ggctggcgac gcgctgattt ggcggccttg gtgccaatga
      421 cccaatcagc gccttcaagt caactcgctc gcttgaagtc agtcggactg atcaagaacg
      481 tctgtgaccc gcacctttgg taactcctgc tttggccata tcgctgccca tgagccgaca
      541 atcaacgctt ttgaggtctg cccgttgtat tcgccaatgt tcgattttga caaccatgcc
      601 gccgcactgg ccagtgccca acagggtttg acgcagttgc accggcctgc tgggatcacg
      661 acgcgcaccg cggccacgcc gtcgagccga tgcggctgct ggtttcggcg cagctctggt
      721 gccatgcctg gtttgctcgc atgagcggat ttaccaggct ggcgggtagc tacagactgc
      781 atccggggca gacacctggc atctccggcc accgaccggt gctgggtcag ggcttgctaa
      841 cgcgcttgtt gcgcgggcaa agtggtgaga cggtagagcg cacactgtct gccctgttca
      901 cactgtgctc acacgcgcat cggcgcacgg cgcggctggt gctcaacgcg gcacgctggc
      961 ctggtcaagc agcgctgcca gagccgccca agctgctgtt gacgctggag accgcgcgcg
     1021 accatctgcg cagcatcgcg ctggactggc cgcgccagct cgtcctgctg gctctgcctg
     1081 accggtcaga tgatggatca aatcaccttc tagcccttat ggaatctgcg caacatgcta
     1141 taaaaataaa ttcatttgac tggctcaaag cctgcccgct ggcgctttcc caaacccacc
     1201 tggcaccgac atggcaagaa gccgagcatg cgctgaccca actgcgtcac tggctcgaag
     1261 tcagcgtgct gcagcagcca ccggacgact ggctagcgca gcactgcacg cccgatgcgc
     1321 tggtacagtg gtgtcgcaac cgctccaccg atttgcctcc ggcacagttt ttggcggctt
     1381 gccatcgaca ggcgagcgca ttgattcccg gagcgcgaac gctcgatgtg ctcgaccacc
     1441 acccgcaaga gcaggcggct aagcttgccg agctggcgcg gcttatcgta gcccagcccg
     1501 actttgcgca gtttccagct tggtgcggcc agtgccttga gacaggcccc tgggcgcgcc
     1561 tgcgccatcg ccgccttgaa cccggcagcg ctgcagctaa caggccaacg gacaagccaa
     1621 gcgcctgggt ccggctggca tcgcgttggc tggagctgat cgagctggcc aatgcgcctg
     1681 tcacccttgg ctcccccccg ccggaacaga aagccattgc agcgccaggc atcgttgcga
     1741 atgacatggc attgcccgct ctcctgggac cgggtaagga ccggcctgcc gcactctcaa
     1801 gtggcgcgtt gaagctgggc gacggccaag ccatcgcgtg gtgcgaaatg gcacgcggcc
     1861 tgctactgca ttgggtacag cttgataccc agggcaaagt ggctgattac cgtgtgattg
     1921 ccccgaccga gtggaatttt catccccagg gcgcgctgtc cctggcggtg agcgccttgc
     1981 caccacaaga caccgcatcg gcatgtattc ttggggctgc ctatgatgcg tgtgtcgaat
     2041 gcagtgtgat cagcagcgga tcaaaatgac aacatcaaga caagaaagac aagatttgcg
     2101 tggtttgcgg aagcggcaat atgccttctg tcacccagcg cgcgcccgga ttggcggtgc
     2161 agcccagctc cggtggcctg cactttgacg cggatgtcgg tgcccggcac gagccagaag
     2221 catgagccgt tcgcgctcac gcctcattgg acaaggcttc acccgcaaat cagcattctg
     2281 tcgtgtcgga atcattctgt cagttgccag caagtccatt gctcgcgaca gtctgtcctt
     2341 cagccataaa cagccattct gaatagctgg ccttgccctc gagattcatg tcccacaagc
     2401 ggaacccggt ttttttcagt ggcaccaggc tggacttgct gtacacgcca acaatctggc
     2461 catccgaaag tttcaccaga ccccaatctg tctgacgcgt catcggatcg atgtagatct
     2521 tgcgcagatg ccgtcggatg gtcggctttc cttggtcctg caccagatcg tcaaggcgca
     2581 tgggatagcg ctgattgatt tggtgatagc ggttaagacc gacccggaac tgatgcccgg
     2641 caaacaacag gtccttttca ttgtcgcgct gcaccatggt gtgccatatg gttcccagcg
     2701 cggccagcat ggctcccatg atggccaccg caatcagtac ggcaatatag gtaaaccccg
     2761 actgcctacc agtcactgta aggggttcca tcggatgagt ttcccggcgc gccgctgtgg
     2821 atatcaaaaa cgccaccctt gtcgggcgta tctggtgcca tgatgatcca ggtcagcgcg
     2881 ctttcggtga ccggatcaac cggcaaattg cgcaagtatt tcttctcgac cagcgtttcc
     2941 aggcgatcgg ggtacttgcc ggtatcacca tagaacttgt cgagcacatc gcgcgtcaag
     3001 gccagattgg cccgcagtac cgtctctttg gacttgtcga gccgatgaaa atacagcggc
     3061 gttgcaatcg acagcagcaa cgcaacaatc gccagaacga ccaccagttc aatcagggta
     3121 aaaccgcgct gggcagggag cgggtgtgaa ccattagcca cagcgttcac cattgctggt
     3181 aggggattcc attgagaccg atgccagttg accaggaata gacatcaaag acatcttcgc
     3241 cttcactcgg cgcatcgggc gggctggcat aactgcgttt gccccagctt gcctcagggg
     3301 gcgcagcggc atcctggaaa aatggatcgc gtgggatgcg cctcaaaaag tacatttttt
     3361 gccctttcct ggcgcttttg acatcatcga caccttgcac cagcgtctgc aggtctggcg
     3421 ggtagccgga cttttgcgcg tccgacagaa tccggccatc atcgaccgcc tgcttatagg
     3481 catcgatagc ggttcgaatc tgctgcagtg cgtcgcgaaa ctcccgttcc ttgctgcgct
     3541 ggatggcaac ttctgccatt ggaaccaaga ccgatgccaa caggcccaca atggccaccg
     3601 taatcaccaa ttcaatcaac gtaaagccgc gctgctggcg gttccgattg aacacagtca
     3661 tggaccgggc tcgatattga cggaaagcgg ctgcggagcc accagcgtca tcgcctcggt
     3721 gccagccgtt gcggcagtgg cactggagag ctttatcaag gctggtcggc ccggctttcc
     3781 ggtcacttcg aattcgatgt gcgcgacggc ccccgtgccg ctggcgactg caccgtcctc
     3841 tgtgaccaaa tccgcatgaa ttgaacccgt cgcttcgtca actcttgcgt caaagttcga
     3901 cttgataccg gtgcggcgca tcagattgcc ttcattgacc gagatcggct tcaaggcttc
     3961 tttgtcgtaa gacaaggtcg catgaaccgc actgagaccg ctgccgccct ggacattcac
     4021 cgcaacgctg atcttttcac ctggattggc ctggattggc ccggacaagg tcagcgtcgg
     4081 tgtcgctgat gccacctcgg ccgcaagtgg ggcaacggct gatgaggcca aagtcgctgg
     4141 tcgtgccgcc tgagatgtcg gagcccggaa tggaatcgag ggattgcgcg cggcggctgc
     4201 accggaggcc gggcttgccc ccttttgagt cagcacaccg gccgaggccg ccactttgag
     4261 gtcaagcggc aaatacttga cattgctttc cgtaccggac cacagttcga gcaaatctgc
     4321 ttcgctcacc tggttgttgc ggatgatgtg aggggtcatc accaacacca attcccggcc
     4381 actccattcg tcatggctga tgccaaacag acgaccaaag accggaacat cacccaggcc
     4441 aggaatcttg ttggcttgcc cgctgctgtc aaagccctgg atcaggccac caagcacctg
     4501 ggtttcgccg tctttcagtc ggagcgtggt ggtggcgttg ttggttttgg tcttgtacgc
     4561 cagggaactg tcaacccgga cttcaccgcc gagggaactc acctccaggt tcaccttgat
     4621 ggcgacactg ccatcaatgt agatcgtggg ctcaacttcg agcttgacac ccgtttcgag
     4681 gtattggatg gtggtgttgt aaaccggact ggtggtcacc ccggaacctg gtacagcagc
     4741 gctggaaacc accggggtgc ggtcaccaat catgattttg gctttttcct tgttgcgcac
     4801 ccggatccgg ggagtcgcca gcgtcctggt gtcggaatcg gtacccttgg ccttgaccga
     4861 catgccaagc gaagacatgt tgacatcggc tgaactgctg ttgcgcaaag cagccagcgt
     4921 cattgggttg atggtgccgc tgaccgacgt tggccagtcg atgccaaggt caatcgctcg
     4981 ggagcggtca acgtcgagta gttcgacttc aagcatcact tcgggttccg gttggtccat
     5041 cgctgccacc agcttttcgg ccagccggat gacttgcacc gtatcgcgaa cgaccaccgc
     5101 attgctgcgc tcgtcaacaa aaatgtcctt gcttttcaaa atcgatttga tcatcgcttg
     5161 aacctgcttc ggatcggcgt tgaccagcgc gaagcggcga accttgagtt cctggtattc
     5221 cttggttttg gcaggtgtga gcgggtaaat caacacggcg ttttcgccca gaatgcgttt
     5281 ttccaactgg ttctgcaaga ggataaggtc cagcgtttct tcaaccgggg tgtccttgac
     5341 aaaaatggaa accttgatgt cattgcgaac atctttgtcg atcacaatat tcaaaccggc
     5401 tgccatcgag atcgcttcga gcaccattct cagattggcg tcccggaagg tcagagtcac
     5461 cggcttgcgc tccttgatat tgagccttgg gccagtcaga ttctccctga gccattgctc
     5521 ttcaagctgg taacgcatct ggttggcttt gacatgggta ggctgctcaa gaagaattag
     5581 gccgacggca gacctggcac ccaccacatc acccagtttg gccatgctct gtgcgtcggc
     5641 cagccacttg aggtggcgct gctcagccag cgcgctgcgc tgaccgcttt ttgccgcctg
     5701 attgttcgga tcaatcgcca gcacacgttg gtaaatcacc tcagtcgcct caaagcgctc
     5761 gttggccaat tcactacgcg cagagttgag caggcgattc accacctgct cgcgttggcg
     5821 gacatagtcg ctgcgtgatt cggcatcttt tggatctgcc tgcgtcacct gctcaagtcg
     5881 tgccagtcct tcctcatagc ggccgtcctt gatcagggac aagccctcgc ggcggccgga
     5941 atcagttgca caacctgcga gccaggcgac aaccagcagg cgcaacatca actgacataa
     6001 atgcgctccc ggccaggctg gacggcgtgc tgcgcaggct tcgaaatgat ggactgactt
     6061 cactgattgg ttcctgtgtc tagcgattgt tcaacgttaa gtggaagata aagaaaagtc
     6121 agttgaccgc cctgcatgct gaccactttg taggttttgt ccatgacatc accgacggag
     6181 acgctaatca tgcggacccc ccgggtcagc agaatcagtt gtttgtcacc ctcgatgtac
     6241 tgcccgaggt aaacaaaggg gagcggcggg gcactgggtg gtggcggtgg cgccggaggc
     6301 ggcggcggcg gcggcggcgg cggcggcggc ggcggcggcg gcggcggcgg cggcgnnnnn
     6361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcaga taccaagata ccccggcaaa
     6481 caagtcagga atattttctg ccaccggcgc acgcccctgg ctgatgagtt gtccatggct
     6541 ggcatccgct gcaccaaccg gtgtcactgt ggtttttgtc ggcctgctgc gcacagcgac
     6601 agccttgcgg ctgcggccgg ttttgtccag cgccggggct gcaaggccgt cttcatcgcc
     6661 aggcgcaaac cagaccagcg ccagcaacac cagcacagcc aaaaccagcc agatgcgccg
     6721 ggtgaccggc ctcatgaacc cttctccaga tacagtcgaa acacgatgcg ggcctcaacg
     6781 ctgccatcac caactttgtc tcgtttcaga tcaaggcctt ccaaagccaa tgccggagcc
     6841 gtcagcagcg cggcccggat gaacttgcgg acttgaggat atgttccctt gaccggcaag
     6901 gtgatggtga acatgtccag ccgccctgcc tgggcctggg tcaaggaata ttcgccgaca
     6961 ttcagtgcca actggtgttg ctcagccagg acgtatattt tccccaacca gtcgggcgtt
     7021 gatgcgtggt cggggaactc ccggtaaaaa gccaagagct gctcggtggc tgtcgtcggc
     7081 ttttgtgctg ctggcgtttg ttgtccctgc ttgagctttg ccgtgaccag cgccagttgg
     7141 cggtcgatct ggtccaaacg agcaccagac aacaatccga tactgacagc ccaaaccagc
     7201 gcggcaacca ccaaaaccag gccgcaaatg ccctgccagc ccagcagtcg cagacgaaag
     7261 aaaagctggg cccgcagcat gccgacattc atgaactggc agcccacttg gcggtgatgt
     7321 gaaaacgaac cggtttttcg ctgtcctgct gattcacctg atgggaatgc agcaccacgg
     7381 cggacaaatc accctgctgt tgaagcattt tgtaaaagcc gagcatcgcc ttcaagctgc
     7441 ggccctcagc ggtcagaaca agttcgcgcc gaacagcgtc tggctccagc gacagtaaag
     7501 ccacttgccc gaccgattga ccttccaaag tggcaaacaa cttctcccag ggaaggttga
     7561 gcgtcccggc aaccgccaag gcctgacccc attcggccgc caactgcacg tcatccattt
     7621 tcggcacaac aggctggcgc gccgccagcg ccatctgtaa ggatcgcaac tgcttctcat
     7681 tggtcgcaat gagcggctgc caatacagac gatcccaagc catcacacca accagagcca
     7741 cgatgccaag caacaggccg acccagcggg ccgcgccggc aggctgtgct cggctggaaa
     7801 aattcagatc aattcgtcgc attctggaag gtccaaaggg ccggaccaaa ccggctgtca
     7861 gtagcagggg caaagttgcg gcgggcattc agggtcaaac tccgaaagga ccagcgggtg
     7921 tttgcaggca tcgatagccc agcaagcgcc gacgaataaa ccagcgcttg cgatgccacg
     7981 ctgtcgatgc cggccaacat cacctcgttg tcaagcaggt ccggcaaaga tgcctgccag
     8041 cccgggccaa cacgcaagcg gcgcaaccat tgccaatggc cacgcttgac cagcccaata
     8101 caaagttgcc cctcttcatg caacaccacc caaccggtga aacgtcgatg aaactgtttg
     8161 cgccagtggt tcacggctgc agcaaagtac ggctcaatag agactaggcg agccttgttt
     8221 gccatcgcgc cctgctcaag tgcggcaaga aaatcagcgt caaccgcggt ggccaccctt
     8281 ggcgagccgg ggacatccaa gctcagacgc acctgccaat tggcagcgac atcaccgtag
     8341 gtttggacaa aggccaggcg agccaccgaa tcctcttcct ggcggtcatg caaatcatca
     8401 cgccatggct gcaactgata ggcggtgaaa tgactggaga gaatcaccca gacccggtga
     8461 caggttgaaa attcagccaa tacaccattg aaagccaggg ccgcaccgct ccagagcgcg
     8521 cctggcagtg cggagaccgg acaaatgcgt ttggcggcaa tttgtgggcg caagccgcac
     8581 cagtgcaccg ccgccagttc ggtgggggac acaaagacgc aaagcgactc aggcgacaaa
     8641 agtgacacga ttgatttccg tcaaagtggt ttcgcccagg cgcaccaggt caagcgccga
     8701 atcccgcagg aaatgcgtac cgttttggcg cgcggcttcc ttgatctggc gaatcggcgc
     8761 acgcgccacg atcaactcgc gcaggttgtc gttgaggtgc agcacctcgg caatggcctt
     8821 gcgccccttg tagccactgc cccggcaatg tccgcagcct tgtccggccc ggaaccgaaa
     8881 acctgcgaca tcctcgcgac tcagccccga atccgccagc aaggcagcac tgggcaggtc
     8941 atcggcaacg cactgcgggc aattgacgcg caacagtcgc tgcgccagca caccattgag
     9001 cgctgacaca aaactgtagg gatcaacacc catgtgcatg aaccggccaa gcacatcaaa
     9061 gacattgttg gcatgcaccg tggtaaacac caggtggcca gtcaacgccg actgaatggc
     9121 gatctgcgcg gtttcggcgt cccggatttc accgaccatg atcttgtcag ggtcatggcg
     9181 caggattgag cgcaggccac gggcaaagct cagtcctttt ttttcattca ccggaatctg
     9241 caataccccc gaaagctggt attcaaccgg atcctcaatg gtgacaattt tttccgtgcc
     9301 gttgttgatc tcggagatcg ccgcgtacag cgtggttgtc ttgccgctac cggttgggcc
     9361 ggtcaccagc agcatgccat agggctcatt ggccaaccgg cgcaaaatgg ccgaaatttt
     9421 ttcatcaaaa cccagactgg tcagttgcag gcctttcaac tggtcagtta gcgattgttt
     9481 gtcaaggata cgcaacaccg cgtcttcccc atggatgctg ggcatgatcg aaacccgcaa
     9541 atcgacttca cgcccaccaa tgctgacacg gaaccgtccg tcctgcggta cccgccgttc
     9601 ggcgatatcg agttcagaca tgaccttgat gcgcgaaatg gtctgctcgg tcaacgcgac
     9661 cgaggcaacc gagccgacca cacccagtac tccatcgacg cgaaatttga tgaccatgcc
     9721 agcgggattg gactccaggt gaatgtcact ggcatccgca cgaagggcat caaaaatggt
     9781 tgaattgacc aagcgaacga ccctgctgct gtcttcactg atgccacgaa aactgagttg
     9841 ctcaactgct tcggttcttg ggccgacctc ttgcgtttcg ggcagcacgg tgtcaaaagc
     9901 ccgcaagctc tcctcatgca ccgattgcca ggccatgata tcggcgcgac tggcgatggc
     9961 cacaaaaatt ggctcgctgg tgcgggactc ggcccaggac tggaccccag catcaaaggg
    10021 gtcagcgacc accagaacca gttggctgtc ggtagcgcgt agcagcaaac aattgcggga
    10081 catcgtctcc ttgaaactga tcacatcaaa ggccgtctgc attgcccgca actgggccat
    10141 cgacaagaca ggcttgcgca ccgtcaaccc caggcgcacc agaaaacgct cctgatcctg
    10201 ccgctcttgc aaaatctcaa ccattgaccg gccggtcgat gcgcacaagg ctcgggcctg
    10261 cgccagttcg cccgccggaa atggcttttc ggtcgggcgc agtgtcaact cgcctgccgg
    10321 ggtcactgga tgctcccggc caactcgaaa atcggaaaat acatcatgac cacaatggcg
    10381 ccaatgacga gcccaatgaa aaccatcaac aggggttcaa acaggcgcac aaaccagtca
    10441 acccagcgcg cgatttcagc gtcgtaatag gaggcgatgc gctccatcat ctcgcccatg
    10501 cgcccggtgc gctcgccaac acgcaacatg cgtaacgaaa ccgcagtagt caaaccacaa
    10561 gagtgcatgg cagaagatat ttgcagaccc tcgcgaattt gactggcgga ttctgacaag
    10621 cggtggcgca agggaccctg caaaagatca cccgtcatct gtagcgctgg catgatcgac
    10681 atgccaccac gcaagagcat acccactgtt cgatagaacc tggccaactg atacaagcgc
    10741 attcgctcgc caatcgccgg gaccgtcgcc aggcggtcga aaatccagcg gcgcgtccca
    10801 ggctggagca accagaaaac cgcaagcacc gtgacgacca acaggccgat cagcaaagtc
    10861 caaccgtgat cgtgaaccag attgccccaa ttgatcagca attgcgacgc aaacggaata
    10921 tcgcctccga ggtcctcgaa aatgcgcgag aagcgcggta ccacatagct gagcaaaaag
    10981 acaatgacaa gaccgccaac accggccagc aacagggggt aaatggcagc actgacgatc
    11041 tttttgcgca ccacatcgag ctgcatctga taagccacat acctggtcaa cgcctcgggc
    11101 aaatcaccgg tcttttcact ggcccgcacg gtggcaatat acagtggcgg gaaaacggca
    11161 ggaaagtttt gtagcgcctg ggacagcgat tggccctcgc agagaaaggt caccagttgt
    11221 gccagcacat ggtgggtttg cgccttgcct tctttttctt tgagggtttc gatcgactca
    11281 acgaccgata aaccggcctc gagcaacatc agcagttcct ggctgaacag ccccagtgga
    11341 aacacgcgag cgtcagaagt ggccatattg gcaagcgtct tggcactttt gacccggaga
    11401 attgaatagc cctgttgacg cgcttggccg gtggcctcag cgacgctgga ggcgtcaaga
    11461 tacagggttg tcgcgcgggc atcaggccgc agaactcgca actcaaaacg catggttcgt
    11521 tgcctgctgc atccgtaggt gcctattccc agttgccaat atcagcatct tcgccggcac
    11581 cgccaagagc gccatcccgc ccgagagaca tgatgtcgta ctcaccgtaa tcgccaggcc
    11641 gcttgtaggt gtatggattt ccccaggggt cagccggcac tttcttcttc aggtacggac
    11701 catcccattt tctttcactg gggggctttt cagtcagcga agtcagaccc tgttcggttg
    11761 tcgggaaatg ccccgtgtca aggcggtact gttccagcgc tttgccaaag gcatcgatct
    11821 gggcgcgcgt tgtcttgatc tcggacttgc caatttgctt gaaatacatc gggccaacat
    11881 agcccaccag caacccgatg atgaccatta ccaccaacag ttccagcagc gtaaagccgc
    11941 gcacgatccg cgtgcttctg attgacatct gacttgtcct atttttttcc gctcggtaac
    12001 acattggagc tgtgagcaaa tgaagaggcg acctgttaag tcacaagcaa ggcgaaatgc
    12061 gctgcaggcg actgcggcgg gttgccttta gcccttggca ttagttgaat aatagccctt
    12121 ccacgacccc aatccgataa accacgaaga cgcctagcag gcaactgaag ccaccaatgc
    12181 ccaagggcag gccctcgtgt agccagccag gcgagcgggc agaccaaccc aatggcacgg
    12241 tgatcactgc ggacagcaag gccattccga cgatggagcc gatgccgaaa atggcaatgt
    12301 acaggagccc catgaaccat gatgattcaa ctcccagact caatgcaatc agcgctgctg
    12361 agccggccaa tccgtgcatc atgccgataa acaaggcacg cagtggcggt ttgtctggat
    12421 gcccgtggct gtgtgccgaa cactcggatg tttgcggccc caggtggctg tgcgcatgaa
    12481 catgcttgag attttgatcg tggctgtgaa catgaacatg tatttgctgc ttagccaagc
    12541 ggcgccagac gtcgaccccc aacatcatca gcatgagtcc cacagcgaat tccagtgcct
    12601 gcgcggtctt tgggggaatg gcggcaccca gccagagggc gatgccgccg atcaacatca
    12661 aggtcagcgt gtggccaacg ccccaggcga caccttgtct gatggtttgt gcgagcgagt
    12721 tctgcccgga gcttaaggtc gccatggcgg ccagatgatc agcatcagtg gcatgtttca
    12781 tacccaggag cagtccgaaa agcagcatgg aaaaggtgct catgttgcct gcgctgtccg
    12841 cgttctgtcg atgtctgact gcaacttgcc gatttccaga agaagcgcca cagtggcggc
    12901 ggcctcgttt tcatcaatgc gcaaccgggc aaacccaaga tgcaccaaca cccagtcgcc
    12961 cagacaggct gacaccggat gctcgtcatc gaccaacaag ctgatgttca ccgcgcgtct
    13021 gacaccgccg acatcgacca ttgccagttg catatcactg ctgatgattt caacgatttg
    13081 tccgggtagc cccaagcaca tggctttctc caatcaattc ttctgaagtg ccagcgacct
    13141 tgccgcagca accaccgcct gccccagagc cagtccgccg tcatgaaatg gcacctggtg
    13201 atgcgtcaac accctgaaac cgacgctctc cagttgcatc atcacagcct ccagcaaaat
    13261 ccggctctga aacagcttgc cagacaaggc aacggtcttg accggctctt ggcgattggc
    13321 gttgccgctg aaatcgtcaa gcatgcggcc aatgaccctc gtcaggtttt tctggaaacg
    13381 tgtcgtggtc tcgccaacca gggcatcaac catcggttcg tcatcgttcg ccatggtctg
    13441 acgtcccccg cctacgccag acttgacgcc cgcatggaca gttccgagcg ctcgacactg
    13501 gcaatagtcg gccaggaaaa atcgaccccc gccagaagag ccatcggcat caaggtccaa
    13561 gccatccagc accacaccca gcacggggct ggcgcaccgg ggaatgccgt tttctgccag
    13621 gcaagcggcg atgtgggcgt gatggtgttg cacttcttca catgccagcc cccggcgctc
    13681 gccccaatcg cgcccccgtt tgcttgacag accttcaggc tgctgatcca ccgcaacaat
    13741 ctgtgggcgg tgttcaaaca gttgcagata actttcaatg gactgcaggt aatcgacccg
    13801 acagcgtgca cctgtcaggt cgcccatgtg gtgcgacatg atcgcctggc cctggcgcaa
    13861 aaggcaaaag ctgttgttca gatcgccgcc catggcaagc accgctggcg catcgtcaaa
    13921 gccttgcggc agcgcaatgg gtgcaggagc ataaccacgc gctcgacgca tcagtcgggg
    13981 caaaccggcc acgacgcgca ccagagaatc atcaacacgg cgggcaactc cacgatcgtg
    14041 cagcaaaaaa tagtccacca tgtcgctcaa gcgagcaagc gcgtcagcat tttcagtgca
    14101 ctgtggttca tcagcgacat tgccactggt caagaccatc gggcgttcca tgcactgcat
    14161 caccaaatgg tgcagcggcg tgttgggtag catgaacccg aggcttgtca cacctggagc
    14221 taccgatgcc gcaagctgtt gggtttctgt ggcatcaaga atgacgatcg gtgaagccgg
    14281 gccttgcagc aagtctgctt caacttggct gatggcacag tagcgccgca ccatcgccag
    14341 atctcgcgcc atcaaggcca ggggcttgcg actgctgcga tgcttgagct gtcgcagccg
    14401 actcactgcg tcctcgttac cggcatcaca ggccaactga aatccgccca gccccttgat
    14461 ggcgacgata aagccgcgct tgatcagggt accggcagcc tcagcatcat cgacagcgtt
    14521 cagcgactgc gggtcaaacc ggccaccccc agccttttcc agccaagtgt gcggaccaca
    14581 tgatgggcag gcgacaggtt gcgcatgaaa acggcggttg cccgggtcgg catattcgtt
    14641 cgcacactgc gcgcaaagcg caaagcttcg catcgtggtt gcacctcggt cataggggat
    14701 ggcctcaatg atggacaagc gtggtccgca atgggtgcaa ttggtaaacg gatagcggta
    14761 gcgtctgtcc aagggatcaa aaatttccgc cagacaaccc gggcaggtcg ccgcgtcggg
    14821 cgtgatgttc gtgtgaacgt ggccgacctg actggcgacg atgcgaaaac cggattcttg
    14881 cgcccggttg gcaatggatt cccgttcaat ggcctcgatg caagcgagtg gcggcgcctg
    14941 tgccggcaag tcatcaacaa aggcacccag cgcggcgaca gaaccacaag ccaggatgat
    15001 gacg
//
LOCUS       DAOSLL010000027        14919 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4137_length_14919_cov_4.248318, whole genome shotgun sequence.
ACCESSION   DAOSLL010000027 DAOSLL010000000
VERSION     DAOSLL010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 14919)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14919)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14919
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4137_length_14919_cov_4.248318"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="PLB25_02025"
     CDS             complement(<1..206)
                     /locus_tag="PLB25_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40403.1"
                     /translation="MLFHHHSPKPLLWVGSAKKDLQAMPSDVQDTFGYALHFAQTGSK
                     HAQAKPLNLDSSVGRARVGRWRRV"
     gene            complement(229..375)
                     /locus_tag="PLB25_02030"
     CDS             complement(229..375)
                     /locus_tag="PLB25_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40404.1"
                     /translation="MPRVCHIAQILLSKARAKVEKHLEDGYLRQVPAPLGVVPPIIAW
                     MALS"
     gene            complement(415..549)
                     /locus_tag="PLB25_02035"
     CDS             complement(415..549)
                     /locus_tag="PLB25_02035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40405.1"
                     /translation="MLGDVQKRQYQLFDNGGENEAEQDEDAIPEEMLAAVELLDRNQR
                     "
     gene            complement(575..868)
                     /locus_tag="PLB25_02040"
     CDS             complement(575..868)
                     /locus_tag="PLB25_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40406.1"
                     /translation="MATKTGNDLFIVDNSDNDWKVLNYLAEWSEISHKFDIATGYFEI
                     GALLALHGKWQKLDQIRILMGDEVTKRTHRALVEGLDGLCEGQQHHQTRAGGF"
     gene            1062..2699
                     /gene="ushA"
                     /locus_tag="PLB25_02045"
     CDS             1062..2699
                     /gene="ushA"
                     /locus_tag="PLB25_02045"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: protein motif:HMM:NF007109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     UshA"
                     /protein_id="HOE40407.1"
                     /translation="MKLNRLCLSLGLIAVLAGCALAPPTGAEKDKTYRVTIMHTNDHH
                     GRFWHNSDGEYGMAARKTVVDEIRRDVAAHGGYSLLLDGGDVNTGVPESDLQDAVPDF
                     KGMNLLGYQAMAVGNHEFDKPLAVLKMQRNLAQFPLLSANIYEAGQRMFAPYKVFDLG
                     GLKVGVMGLTTEDTVRMAHPDNVGRLEFRSAIAEAGKLVPELRRQADVVIAATHIGHY
                     ENGEHGSQAPGDVEMARAVSGIDLVVGGHTQNPACMKAENVLDHAYVPGAACQPDRQN
                     GAWIVQAHEWGKYVGRADFEYRNGDFKLVKYALIPINLKKSVVGADGNTTLLPYTRVI
                     AQDQEMLALLTPFQQLGQDRLGVQIGTVDARLVGDRDVVRKSLTNLGVLVGRAMMDKT
                     RADFAVVNAGGLRDSIPAGIITYKDVLKVHPFGNTVCTAELSGTEVLGYLNVLAKMSP
                     GSGAYPQFAGIELDISAGAVSNVRIRGSALESGKTYRITVNNFQAAGGDGYPRLSTHP
                     SFVNTGFVDADVLRAYIAAKSPLKAAEYEPGQTVLRR"
     gene            2730..2879
                     /locus_tag="PLB25_02050"
     CDS             2730..2879
                     /locus_tag="PLB25_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40408.1"
                     /translation="MAKGQQNNNKMVKKPKKDTSPPKPVSADAVRPTITTVVPVRGKL
                     KNAPH"
     gene            3089..3736
                     /locus_tag="PLB25_02055"
                     /pseudo
     CDS             3089..3736
                     /locus_tag="PLB25_02055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
     gene            3829..5007
                     /locus_tag="PLB25_02060"
     CDS             3829..5007
                     /locus_tag="PLB25_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE40409.1"
                     /translation="MRESQKMQAIGTMAGGIAHDFNNIISAILGNVELARQDAQAESA
                     VATSLDEIDKAGRRARDLVRQILAFSRNEPPRRVPVRLADVVQETMRLVKVAMPPQLV
                     LQLDIDPATPPVLADATQIEQVLLNLCTNAIHAMAQRAGVIRIELGRASRWPYDGTER
                     RAGLRGKHVRLTVSDTGCGMDEATLQRVFEPFFTTKPVGQGTGLGLSVVHGIMRAHSG
                     NVLVESTPGIGSAFTLNFPVSDEPTQLAQVASSVATLASSSAAPGQRVMYVDDDEALV
                     FLVRRLLTRKGYTVLAFTDPRQAIAALRADPLQCDLLVTDFNMPGYSGVDLLRDVRMI
                     RPDLPMALASGYVTHEIERDALAAGARVLIHKPNDVDELCRTVQQLLAASQTGGAAVG
                     "
     gene            5000..5653
                     /locus_tag="PLB25_02065"
     CDS             5000..5653
                     /locus_tag="PLB25_02065"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HOE40410.1"
                     /translation="MADFSLIYADDALLVLDKPAGLLSVPGRGDDKQDCLSCRVQQLY
                     PDACVVHRLDMATSGLLLMARGSAVQRQLNAAFASRAVHKRYEAVVDGVLTPLKPPDS
                     DGWSAINLPIRLDWPNRPKSKVDMLHGKPSLTRWRVLSSHAAKQTTRLTLQPVTGRSH
                     QLRVHLQAISHPILGDALYASEQVVAKASRLLLHATELELPHPNSLVPMRFISPPPF"
     gene            complement(5655..5972)
                     /locus_tag="PLB25_02070"
     CDS             complement(5655..5972)
                     /locus_tag="PLB25_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HOE40411.1"
                     /translation="MQVQIHTDNHIEGTEAMAQWASNTVKTALDRFANQITRVEVHLR
                     DENAGKKNTAQSIQCTMEARLEGHQPLALKHQGANLNQAIEGAAEKMSRLIDSTLGRV
                     ART"
     gene            6098..6850
                     /locus_tag="PLB25_02075"
     CDS             6098..6850
                     /locus_tag="PLB25_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE40412.1"
                     /translation="MTHLYIITGASRGMGLAMAQQLLSPSHRLLCMSRHVSAGLTDEA
                     FQAGADLTQWSADLVDAAPLTDRLGQWLAAQSAPGIASATLINNAGVIAGIVPLRAAD
                     PLELTRSLRVGLEAPMLLTAAFLAATQAWTATRKVLNISSGLGRRPMASQSAYCAAKA
                     GLDHFTRCLAQDEVRQANGARVCALAPGVVDTDMQVQLRNAAPAGFPDQASFAQLKQT
                     GQLTSPQDAAARVLAYLHRADFGSQPVADVRS"
     gene            6960..8147
                     /locus_tag="PLB25_02080"
     CDS             6960..8147
                     /locus_tag="PLB25_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HOE40413.1"
                     /translation="MTREVVIVSAARTAIGTYGGSLKDLTPTELASQIVREVLSRAQV
                     KGDEVDHVVFGQVINTAPEDMYLARVAAIQGGCPVGVPAFNVNRLCGSGLQAILSAGQ
                     SILLGDADIAIGGGAEAMSRAPYASLNLRWGARMGDTPMVDMLVSTLRDPFHNIHMGI
                     TAENIAAKWGISRADQDALAVASHNRAQHATEAGYFKSQIMPVTLKSRKGNVIFDTDE
                     HFRPNCTLADLARLKPAFLQENGTVTAGNSSGINDAAAAVVLMAASTAKERGIKPLAR
                     LVAYAYAGVEPQYMGIGPVPASQMALKKANLSVVDMDVIEANEAFAAQACAVILDLGL
                     DPAKVNPNGSGISLGHPVGATGTLITVKALYELERIGGRYALVTLCIGGGQGIAAIFE
                     RQG"
     gene            8176..10110
                     /locus_tag="PLB25_02085"
     CDS             8176..10110
                     /locus_tag="PLB25_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HOE40414.1"
                     /translation="MPTTRHWSLGAKLMLVGVPFVLLVLLATAATLWVSWQLDGGAAA
                     VNEAGRMRMQSYRMSLSIGTQETDQLAGQIKEFDHSLATLRQGDPERPLFVPWDEVVS
                     AHFALVEADWVQYQSRWISQRPAVFRDLRDDTMRFAADIDAFVGGIEAHIARWTALLH
                     LLQMSMLALVILAAAILLYAGYLFVLEPVGRLKQAIEKVQGGDFEARVERTSSDEFGT
                     LADGFNDMAGHLQSMYRNLEAKVAEKTVQLQEKSERLESLYEVTALVANAISLDSMAQ
                     GFVNSVARIALADGVALRWSSQGNQRHLMLAAYGLPISMVDAEQCIYTGDCHCGSSST
                     GSNIKVIPIRNTNPANLPHCAKAGFETIVSIPVRLHERLMGEVDLFFHARVEPSESER
                     SLLEALSKHLASGMEYLRLNALEKEAAVSQERHLLARELHDSIAQSLAFLKIQVQLMR
                     DAVKGGDPVEMAKVLEEIDLGVRESYSDVRELLLHFRTRTNVEDVEPALVTTLQKFEL
                     QSGIKASLNMQGQGMPLSPDLQIQVLHIIQEALSNVRKHARASQVRLDVQQQPAWRFE
                     VRDDGIGFDQDRDQPDQTHVGLRIMAERAQRIGAGLVVVSRLGQGSSIILTLPTPALP
                     MSGAAMLRASLPEQASTTTA"
     gene            10118..10828
                     /locus_tag="PLB25_02090"
     CDS             10118..10828
                     /locus_tag="PLB25_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE40415.1"
                     /translation="MNSTLNPIRILVVDDHTLFRRGLTALLSRDPQLDVIGDAADAGQ
                     AIRKAQELQPALILLDNHLPGVHGVDALPALREAAPKAVILMLTVSEDENDLAAALRG
                     GAAGYLLKTIEGEALTSAILSAMQGDSVVSPEMMSKLVAAYRGATRTLSADTYADKPM
                     GKVADVDPPEFVLNCLSPRELDVLRGIARGASNKEIARDHGIAETTVKIHVQHVLRKL
                     DVSSRVHAAVIATEQGLL"
     gene            complement(10874..12055)
                     /locus_tag="PLB25_02095"
     CDS             complement(10874..12055)
                     /locus_tag="PLB25_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase family protein"
                     /protein_id="HOE40416.1"
                     /translation="MSKEVVVLSAVRSAIGTFGGSLANMEPADLATVVMKEAIARSSV
                     DAALIGNAVVGTCIPTDGRYAYVSRVASIQAGMPMDSIAMQVSRLCSSGLQGIVTAAQ
                     SIMLGDFDYSIGGGVEVMSRGAYTLPAMRNGARMGDTKSIDLMVAILTDPFGAGHMGM
                     TAENLATKWNISRQEQDALACESHRRAAAAIADGRFKSQIVPIVQQTRKGDVVFDTDE
                     HVKANTTMETLAKMKPAFKKDGSVTAGNASGINDGAAFFVLASADVAAKAGQKPMARI
                     VSYAVTGVPNDIMGEGPISATTQALKKAGLTLAQMDVIEANEAFAAQAIAVSKGLGLD
                     PAKTNVNGGAIALGHPIGCSGAFIATKTVYELQRTGGKYGLATMCIGGGQGIAVIFER
                     L"
     gene            12507..14381
                     /locus_tag="PLB25_02100"
     CDS             12507..14381
                     /locus_tag="PLB25_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOE40417.1"
                     /translation="MFGKARSKKPSPLDAYADKVTTPALAVTHIRSGDHVFVGSGCAA
                     PRALLQALESMPGLPADLELLHFFTVKAFDHDADGHSTTRLRHRTFFVSSDMRAAVKQ
                     GMVEYVPMSIARVPDMILLGRIPVEVALIQVSVPDEFGYVSLGVSVDIIPAALARARL
                     VIAEVNPNMPRSMGDSTLHVSRIHHLVPVATLIIELQRMPRQDRDVQRIARYIAGIID
                     DGSTLQIGMGQLAHEALQYLTDRKDLGIHSDVVSDAIIPLLENGNLTGAKKTRQPFKI
                     VTSMALGSRRLYRLIDRNPLFSFQPIDSICNLLTISKQHKMVSIAQAFAIDLTGQVCI
                     DQFDGEFYSGIGCQGEFLLGAAQSPGGKPIICMTSTTDDGLVSRIRPALLEGESATIA
                     RSDVHYLVTEYGIAYLFGKSIRQRATALIELAHPKFRPELFAKAQAMGYLSAEQSLHN
                     LRAYPVEEEQTVLLKDGRSVLLRPALSSDAQGIRELFHHLSEADVFTRFFRNVRGLSD
                     AEVERLCNVNYENEVAFVAISGSREESTIVGQSCYFIDPTTNMADVAYMVHPQWQACG
                     LGSAMQACMIRHATRRGLRGFVADVLLGNAKMLRLARNSSLRVQAEKNQDAVHITILF
                     "
     gene            14514..>14919
                     /locus_tag="PLB25_02105"
     CDS             14514..>14919
                     /locus_tag="PLB25_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE40418.1"
                     /translation="MTKRGQALSVLIVSTFAFTVCFMVWMMFAVIGIPIKKTLGLNAT
                     EFGLLTAMPVLTGSLIRVPLGIWTDKFGGRIVMALLMASTIPAIWLMSYATQYWHYLT
                     IGLFVGLAGGSFSVGTPYVARWFPKSQQGFAMG"
BASE COUNT         3064 a         4428 c         4345 g         3082 t
ORIGIN      
        1 cagacgcgcc gccagcggcc cactcgggcg cgtccaactg aggaatcaag gttcaagggt
       61 tttgcctgtg catgcttgct acctgtctgg gcaaaatgca aggcataacc aaaggtgtct
      121 tgcacatcgg aaggcatggc ctgcaaatct tttttggccg agccaaccca cagcaaaggc
      181 ttggggctgt gatggtgaaa aagcatggca aattatatcc aaataggtct aactcagtgc
      241 catccaggca atgatcggtg gcaccacgcc caagggggct ggtacctgcc ggaggtagcc
      301 gtcttcaagg tgcttttcca ccttggcgcg agcctttgaa agcaggattt gcgcgatgtg
      361 gcaaacgcgt ggcatcctgc aggttcaggc cttcgctgag tacgtcggtg gcgatcagcg
      421 ttggttgcgg tcgagcaact ccaccgccgc cagcatttct tcgggaatag cgtcttcgtc
      481 ttgctcggct tcgttttcgc cgccattgtc aaaaagctga tactggcgct tttgcacgtc
      541 gcccagcact tcttcctggt ggatgcgcta gcgctcaaaa gccgcctgct cgtgtttggt
      601 ggtgctgttg accttcacaa aggccatcaa gaccttcaac cagcgcacgg tgggtgcgct
      661 tggtgacctc gtcgcccatc aggatgcgaa tttgatccag cttttgccac ttgccatgca
      721 atgccagcaa ggcgccgatt tcaaaataac cggtcgcaat gtcaaatttg tgtgaaatct
      781 ctgaccattc agccaggtag ttcagaactt tccagtcgtt atcgctgttg tcaacgatga
      841 aaaggtcgtt ccctgttttt gttgccatct tctttgccgt ttaactcata tgctccaatg
      901 tactgcaaac ctcaaaggtg ccctgaaacg ttcgccatac gcagatttca tcgtccagcg
      961 gggagacaac ccgccctgtc aatactcgac cagaggcctg tattgacaga tgcccagtgc
     1021 tgccaaaatc aacatcgttc aatacacacg aggaaacctt catgaagctc aaccgacttt
     1081 gcctgtcatt gggcctgatt gccgtgctgg cgggctgcgc gctggcacca ccaacaggtg
     1141 ccgaaaaaga caaaacctac cgcgtcacga tcatgcacac caatgatcac catggccgct
     1201 tttggcacaa cagcgacggt gaatacggca tggcggcgcg caagacggtg gtcgatgaga
     1261 ttcgccgtga tgtcgcagcc catggtggct acagcttgct gctcgacggt ggcgacgtca
     1321 acaccggcgt gcccgaatcc gacctgcagg acgcagtgcc tgacttcaaa ggcatgaacc
     1381 tgctgggcta ccaggcaatg gcagtgggca accacgagtt tgacaagcca ctggcggtat
     1441 tgaagatgca gcgcaacctg gcgcagttcc cgctgctgtc agccaacatc tacgaagctg
     1501 gccagcgcat gtttgcaccc tacaaggtgt ttgacctcgg tggcttgaag gttggcgtga
     1561 tgggtctgac gaccgaagac acggttcgca tggcacaccc ggacaacgtc ggtcggctgg
     1621 agtttcgtag cgccatcgcc gaggccggca aactggtgcc agagctgcgg cgccaggcgg
     1681 atgtggtgat tgccgctacc cacattggcc actacgaaaa cggtgagcac ggcagccagg
     1741 ccccgggcga tgtcgaaatg gcgcgcgcgg tgtccggtat tgacttggtg gtcggcggtc
     1801 acacccaaaa cccggcctgc atgaaggccg aaaacgtgct cgaccacgcc tatgtgccgg
     1861 gtgcggcgtg tcagccagac cggcagaacg gtgcctggat cgtgcaggcg cacgaatggg
     1921 gcaagtatgt ggggcgggct gattttgagt accgcaacgg tgacttcaag ctggtgaaat
     1981 acgcgctgat cccgatcaac ctgaaaaagt cggttgtcgg tgctgacggt aacaccacac
     2041 tgctgccgta cacccgcgtc atcgcgcaag accaggaaat gctggcgctg ctgacaccgt
     2101 ttcagcagct cggccaggac cggctcggcg tccagatcgg caccgtggac gcgcgcctgg
     2161 tgggtgatcg cgatgtggtg cgcaagagcc tgaccaacct gggggtgctg gtcggtcggg
     2221 ccatgatgga caaaacccgc gctgactttg ccgtggtcaa cgcgggtggc ctgcgcgact
     2281 caatcccggc gggcatcatc acctacaagg atgtgctcaa ggtgcatccg tttggcaata
     2341 ccgtgtgcac cgccgagctg tcgggcaccg aggtgctggg ctacctgaat gtgctggcga
     2401 aaatgagccc tggttccggg gcttaccccc aatttgcagg cattgaactc gacatcagcg
     2461 caggtgccgt cagcaatgtg cgcatcaggg gcagcgcact ggaaagtggc aaaacctacc
     2521 gcatcacggt caacaacttt caggccgcag gcggcgacgg ctaccccagg ctgagcactc
     2581 acccgagttt tgtcaacacc ggctttgtcg atgccgatgt gctgcgagct tacatcgccg
     2641 caaaaagtcc actcaaggcg gccgaatacg agcccggcca gacggtgctg cgccgataat
     2701 cacatcagtt tcaacccagg agaaagagca tggcaaaagg tcagcaaaac aacaacaaga
     2761 tggtcaagaa gcccaaaaaa gacacctccc cacccaagcc agtatcggct gacgcagtgc
     2821 gcccgacgat taccacggtg gtgccggtcc gtggcaagct caagaacgcg ccgcattgac
     2881 ccctgtcaga ttacgccagc tgggtagccc gcagcgccgc tcaagcgctc aggctggcag
     2941 ctattccaat gccggcctgg ttgaagacct gcggcgctcg caggctgaac tggagattca
     3001 aaaccgcgcc ctgcgcttta gccaggctgc tgccgaaggg gcctcggacc gttttgtcac
     3061 gctgttttcc agtgtgccgc tggcgctgat ggtggtcgat gaacacggcc agattctgga
     3121 acataacgcg cgggcattgg cactgttgcg ccccctggaa gacgacccgc cgctcaattt
     3181 cctgttcccg ttggtgcatg gcggacacgt caggcaagtc caggacggtt ttgtctcggc
     3241 caacgaaaat ggtgcctgcg aactcagtga actcgagttt gccgccggtt ccaatggttt
     3301 ttttaccggc gacctgcaca ttgcgcgcat tgacaatgcc caggacgact tggtcaactt
     3361 catttgcgcc gtcatcgacc aaagcccact gctggcgcag cgtcaggcct tgcaagccag
     3421 tgcgcaggcc ctgcaagaac gcaatgacga gttggtgcat agcaaggccc ggctcagcgc
     3481 catcatcgac tcctcacttg atgccatcat ctgtgttgac cagcaccagc gcatcattgt
     3541 tttcaatccg accgccgcca ccctgttcca ctgcccggcc gatcaggcct tgggccagcc
     3601 gttgacgaag tttttgccgg atgccgacag cgcactgtcc taccgcgaca tagccagccc
     3661 tgcccagctt ggcgagatga ccggtgttac cagcgagggt gacaaggtgc cactggatgt
     3721 cagcctgtcg tttgagcacc gccatgatgg cgacatcatc accttgtttg ctcacgacct
     3781 gaccgccacc aagaggatgg ggcacatcgc acggcacttg agtcacagtt gcgcgaatcg
     3841 cagaaaatgc aggccattgg caccatggcc ggcggcattg cgcatgactt caacaacatc
     3901 atcagtgcca ttttgggcaa cgtggagctg gcccgccagg atgcccaggc cgagtctgca
     3961 gtggccacca gcctggacga aatcgacaag gctggtcggc gcgcgcgcga tctggtgcgg
     4021 caaatcctgg ccttcagccg caacgagcca ccccggcgcg tgccggtcag gctggctgac
     4081 gtggtgcagg aaacgatgcg gctggtcaag gtcgccatgc caccccagct tgtcttgcag
     4141 cttgacatcg atccggccac gccaccggtg ctggccgatg ccacccaaat tgagcaggtg
     4201 ttgctgaatc tatgtaccaa tgccattcac gccatggcgc aacgtgccgg tgtcatccgg
     4261 attgagcttg gccgtgcatc gcgctggccc tacgacggca ccgagcgccg tgctggcttg
     4321 cgcggaaagc atgtcagact gaccgtgagc gacaccggct gcggcatgga cgaggccacc
     4381 ttgcagcgcg tctttgagcc ctttttcacc accaagccgg ttgggcaagg caccggactc
     4441 gggttgtcgg tggtacacgg catcatgcgc gcgcactcag gtaacgtgct ggttgaaagc
     4501 acgcccggta ttggcagcgc ctttaccctg aactttccgg tttcagatga acccacacag
     4561 ttggcccagg ttgcatcctc ggtagcgacc ttggccagtt ccagtgccgc accaggccag
     4621 cgggtcatgt atgtggatga cgacgaggcg ctggtgtttc tggtcaggcg cctgttgaca
     4681 cgcaagggct acacagtgct cgcgtttacc gaccccaggc aggccattgc cgccctgcgt
     4741 gccgacccgc tgcaatgcga cctgctggtg accgacttca acatgccagg ctacagcggg
     4801 gttgacctgt tgcgtgatgt gcggatgatc cggcccgacc tgccgatggc actggcatcc
     4861 ggttatgtca cgcacgagat cgagcgggat gccctcgctg ccggggctcg cgttctgatc
     4921 cacaagccca acgatgtgga cgaactgtgc cggaccgtgc agcaactgct ggccgccagc
     4981 cagactggtg gcgctgcagt tggctgattt ttctctgatc tacgcagacg acgcgctgct
     5041 ggtgctggat aagcctgccg gactgctcag cgtgccgggt cgcggtgacg acaagcagga
     5101 ctgcctgagc tgccgcgttc agcagcttta cccggatgcc tgcgtggtcc accgcctgga
     5161 catggccacc tcgggcctgt tgctgatggc gcggggcagc gccgtgcaac gtcagctcaa
     5221 tgccgccttt gccagtcgcg ccgttcacaa acgctatgaa gcggtggtgg atggcgtgct
     5281 gacacctttg aaaccgcctg acagtgacgg ctggtcggca atcaacttgc cgatacggct
     5341 ggactggccc aaccgcccga aaagcaaggt tgacatgctt cacggcaaac ccagcctcac
     5401 ccgatggcgg gtgctgtcaa gccatgcggc aaaacaaact acgcgactga cgctgcagcc
     5461 ggtgaccggc cgctcacacc aactgcgcgt gcatctgcag gcgatcagcc atccgattct
     5521 tggggacgct ttgtacgcat cggaacaggt cgtggcaaaa gccagtcgcc tgctgctgca
     5581 tgccaccgaa cttgaattgc cccaccccaa ctccctggtg ccaatgcgtt ttattagccc
     5641 gccaccgttt tagctcaggt gcgtgcaacc cgcccaaggg tgctgtcaat caggcggctc
     5701 attttttctg cagcaccttc gatcgcctgg ttcaaattgg caccctggtg tttcagcgcc
     5761 aggggttgat gcccttcaag ccgggcctcc atggtgcatt gaatgctttg cgcggtgttt
     5821 ttcttgccgg cgttttcgtc gcgcaaatga acctcgacgc gagtgatctg gttggcaaag
     5881 cgatccagtg cggtcttgac cgtgttgctg gcccattgcg ccatggcctc tgtgccttca
     5941 atgtggttat cagtgtgaat ttggacttgc atgttgtttt ccttttaaag taggcaccgt
     6001 gaaccctgtc catcatatcc accaaggggc ctgattgaat tgactgtggt caacactctg
     6061 gcaaactgat gccggccaac ccaccacgca tagcaagatg acccacctct acatcatcac
     6121 cggtgcctca cgcggcatgg ggctggccat ggcacagcag ttgttgagtc ccagccaccg
     6181 gctgctgtgc atgtcacgcc atgtgtctgc cggtttgacc gatgaagcat ttcaggccgg
     6241 tgccgacctg acccaatggt cagcggattt ggtcgatgcg gcaccgctta ccgatcgtct
     6301 ggggcaatgg ctggcagcgc aatccgcccc aggtattgcc agcgccaccc tgatcaataa
     6361 cgccggtgtc attgcgggca ttgttccctt gcgagccgct gatccgcttg agttgacgcg
     6421 gtcgctgcgc gttggcctgg aggccccaat gttgctgacc gccgcgtttc tggcggcgac
     6481 acaggcttgg accgccactc gcaaggtttt gaacatctcg tcgggcctgg gtcgtcgccc
     6541 gatggcatcc cagtcggcct actgcgctgc caaggctggt ctggaccatt tcactcgctg
     6601 cctggcgcaa gacgaagttc ggcaagccaa cggtgccagg gtttgcgcat tggcaccggg
     6661 cgtggttgac accgacatgc aggtgcaatt gcgcaatgca gcaccggcgg gctttccgga
     6721 tcaggccagt tttgcccagc tcaagcaaac tggccagctc accagccccc aggacgctgc
     6781 cgcgcgcgtg ctggcttatc tgcatcgcgc agattttggc agccagcccg ttgctgacgt
     6841 gcgcagttga atcgacccac ccggtgccac cctgttttag agaggcccgc tgatgcccag
     6901 cctgccgggc gggctaagat cgtcaggttt cgattcttag cctgaactgg agactgttga
     6961 tgacccgcga agttgtgata gtgagtgccg cacgcacagc cattggtacc tatggtggta
     7021 gcctgaaaga cctgaccccg accgaactgg cgagccagat cgtgcgtgaa gtcttgtcgc
     7081 gcgcccaggt caagggcgac gaggtggacc atgtggtgtt tggacaggtg atcaacactg
     7141 cacccgagga catgtacctg gctcgggtgg cggccatcca gggcggctgc ccggtcgggg
     7201 tgcccgcctt caacgtcaac cgcttgtgcg gctctggttt gcaggccatt ttgtcggctg
     7261 gccaatccat tttgctgggt gatgcagata ttgccatcgg cgggggtgcc gaagccatga
     7321 gccgggcacc ctacgccagc ctgaatttgc gctggggtgc ccgcatgggg gacacgccga
     7381 tggtggacat gctggtcagc accttgcgcg acccgtttca caacattcac atgggtatta
     7441 cggccgaaaa cattgctgcc aagtggggca tcagccgtgc tgaccaggat gcgctggcgg
     7501 tggccagcca caaccgtgcc cagcacgcca ctgaagccgg gtatttcaag agccagatca
     7561 tgccggtgac gctcaagtcc cgcaaaggca acgtgatttt tgacaccgac gaacacttcc
     7621 gacccaattg cacgctggct gacctggccc ggctaaagcc tgctttcctg caagaaaacg
     7681 gcactgtcac ggcaggcaat tcgtccggta tcaacgatgc agcggcggct gtggtgttga
     7741 tggctgccag cactgccaaa gagcggggca tcaagccgct ggctcgtctg gtggcttatg
     7801 cctatgcggg tgtggagccc caatacatgg gcattggtcc ggtacccgca tcacaaatgg
     7861 cgcttaaaaa ggccaatctg agcgtggttg acatggacgt gatcgaagcc aatgaagcct
     7921 ttgccgcgca ggcctgtgcc gtcatccttg atcttgggct cgatcccgcc aaagtcaatc
     7981 caaatggctc aggcatctcg ctgggtcatc cggtcggggc cactggcacg ctgatcaccg
     8041 tcaaggcgct ttatgaactc gaacgcattg gcggacgcta cgcactggtc accctgtgca
     8101 ttggcggtgg ccagggcatt gcagccattt ttgaacgaca gggctaagcg cccggtgcac
     8161 cagtagcgct gaaacatgcc aaccaccaga cattggagcc tgggtgccaa attgatgctg
     8221 gtgggggttc cctttgtatt gctggtgctg ctggccacgg ctgccacgct atgggtgtcc
     8281 tggcagcttg acggcggtgc agcggcggtc aacgaagcgg ggcgcatgcg catgcagtct
     8341 taccgcatgt cgctttcaat tggtacccag gaaaccgatc aactcgccgg gcagatcaag
     8401 gaattcgacc acagcctggc gacgttgcgg cagggcgacc cggagcgtcc gttgtttgtc
     8461 ccgtgggacg aggtggtcag tgcgcacttt gccctggtgg aggcggattg ggtgcaatac
     8521 cagtcccgct ggattagcca gcgtccagcg gtgtttcgag atttgcgtga cgacaccatg
     8581 cggtttgctg cggacattga tgcgtttgtc ggcggcatcg aggcacacat tgcgcgctgg
     8641 acggccttgt tgcacctgct gcaaatgagc atgctggcgc tggtcatact ggccgccgcc
     8701 attctgcttt atgctggcta tctttttgta ctggagccgg tcggccgcct caagcaggcg
     8761 attgaaaaag tccagggtgg cgactttgag gcgcgcgtcg agcgcaccag cagtgatgaa
     8821 tttggcaccc tggccgacgg cttcaacgac atggcagggc atctgcagtc gatgtaccgc
     8881 aatctggagg ccaaggtcgc agaaaaaacc gttcagttgc aggagaaatc tgaacgattg
     8941 gaaagtttgt acgaggtgac tgcgctggtc gccaatgcca tttcgctcga cagcatggcc
     9001 cagggtttcg tcaacagtgt ggccagaatc gcgcttgccg atggcgttgc gctacgctgg
     9061 tcgagtcagg gtaaccagcg ccacctgatg ctggcagcct atggtttgcc gatcagcatg
     9121 gtcgatgccg agcagtgcat ttacaccggc gactgccatt gcggcagcag cagcaccggg
     9181 agcaacatca aggtcattcc gatccgcaac accaacccag ccaatctgcc acattgcgcc
     9241 aaagctggat ttgaaaccat cgtctccatt ccagtgcgtt tgcacgagcg cctgatgggc
     9301 gaggttgacc tgtttttcca cgcccgtgtg gaaccttctg agtcagaacg atcgctgctg
     9361 gaggcactga gcaaacatct ggccagtggc atggaatact tgcggctcaa cgcacttgaa
     9421 aaagaggcgg cggtgtcgca ggagcggcac ctgctggcgc gggaactgca cgactcgatt
     9481 gcccagtcgc tggcatttct gaaaattcag gtgcaactca tgcgcgatgc cgtcaaaggc
     9541 ggtgacccgg ttgaaatggc aaaggttctg gaggagattg atttgggcgt gcgcgaaagc
     9601 tacagcgatg tgcgcgaact gctgctgcat tttcgcaccc gcaccaacgt ggaagatgtt
     9661 gaaccggcac tcgttaccac actgcaaaag tttgaactgc aaagcggcat caaagccagc
     9721 ttgaacatgc aggggcaagg catgccgctg tcaccggacc tgcagatcca ggtgctgcac
     9781 atcattcagg aagccttgtc gaatgttcgc aaacatgcgc gggcttctca ggtccggctt
     9841 gatgttcagc agcaaccggc ctggcgcttt gaagtgcgtg acgatggcat tggatttgac
     9901 caagaccgcg atcaacccga ccaaactcat gtgggcctgc gcatcatggc cgaacgcgct
     9961 caacgcattg gtgccgggtt ggtggttgtc tcaaggcttg ggcaaggcag ttccatcatc
    10021 ctgacattgc cgacaccagc cctcccaatg tccggggcag caatgctgcg agcgagcctt
    10081 cctgaacaag cgagcacaac aacggcatga ccaagcaatg aacagcaccc tgaacccgat
    10141 ccgcatcctg gtcgttgatg accataccct gtttcgccgt ggtctgacgg cgctgctgtc
    10201 gcgcgaccct cagttagatg tcattgggga cgccgccgat gccgggcaag cgattcgaaa
    10261 ggcgcaggag ctgcagccgg cgctgatcct gctggacaac cacctccccg gcgtgcatgg
    10321 tgtcgatgca ctgccagcac tgcgcgaagc ggctcccaaa gcggtgattt tgatgttgac
    10381 cgtcagcgag gacgaaaacg atctggcggc agcattgcgc ggtggtgcag caggctacct
    10441 tctcaagacg attgaaggcg aggccctgac cagcgcaatt ttgagtgcca tgcaaggtga
    10501 cagcgttgtg tcaccggaga tgatgagcaa acttgtggcc gcttaccgtg gtgccacccg
    10561 caccttgtca gccgacactt atgcggacaa gccgatgggc aaagtcgccg atgttgaccc
    10621 gcctgaattt gttctgaact gcctgtcacc ccgggaactt gacgttttgc gcggtatcgc
    10681 ccgtggtgcc agtaacaagg aaatcgcccg tgaccacggc attgccgaga ccacggtgaa
    10741 aattcacgtg caacatgtgc tgcgcaaact agatgtcagc tcgcgcgtac acgctgcggt
    10801 gattgccact gaacagggct tgctctagcg cctgaccagt ggcccattga cggcaggtgc
    10861 gccaatgatc agatcaaagg cgctcaaaaa tcactgcaat gccctggccg ccaccaatgc
    10921 acatggtggc caggccatac ttgccgccgg tgcgctgcag ctcatagacc gtcttcgtgg
    10981 caatgaaggc acccgagcag ccgatcgggt gacccagcgc aatggcacca ccattcacat
    11041 tggtcttggc cggatcgagt ccaagaccct tgctcacagc aatcgcttgt gcggcaaacg
    11101 cttcgttggc ttcaatcacg tccatctggg caagcgtcag accagccttc ttgagcgcct
    11161 gtgtggtagc cgagatcggg ccttcaccca tgatgtcgtt aggcacaccc gtcactgcat
    11221 aagagacgat gcgggccatt ggcttctgac cggctttggc ggccacatct gcgctggcga
    11281 gcacaaaaaa tgcagcacca tcgttgatgc cagaggcatt gcctgcggtc actgagccgt
    11341 ctttcttgaa cgctggcttc atcttggcca gagtttccat ggtggtattg gccttcacat
    11401 gctcgtcagt gtcaaacacc acgtcgccct tgcgggtttg ctgcacgatt ggcacgattt
    11461 gtgatttgaa gcggccatcg gcaatggcag cagcagcgcg gcgatgcgat tcgcaggcca
    11521 gtgcgtcttg ctcctggcgc gaaatgttcc acttggtggc cagattttct gccgtcatgc
    11581 ccatgtgacc ggcaccaaac gggtcagtca gaatggcgac catcaagtcg attgatttgg
    11641 tgtcgcccat gcgcgcaccg ttacgcatgg cgggcagcgt gtaagcaccg cgtgacatca
    11701 cttcaacacc gccaccgatg ctgtaatcaa aatcgcccag catgatgctt tgtgcagcgg
    11761 tcacgatgcc ttgcagacca gatgagcaaa ggcggctgac ctgcatggcg atggaatcca
    11821 tcggcatgcc agcctgaatc gaggccacgc gcgacacgta agcgtaacgg ccatcggtgg
    11881 gaatgcaggt accaaccacg gcgttgccaa tcaacgcagc gtcaacgctg gagcgcgcaa
    11941 tggcttcttt catgaccaca gtggccagat ccgctggctc catgttggca agagagccac
    12001 caaaggtacc aatggcagaa cgaactgcgc tcaagactac aacttcttta ctcatgggaa
    12061 atctccttaa aattgaattt actgtctgac tgattcgcgg aatgccagaa aaatggcggt
    12121 tgcaaccata acaccattcg actcggaaaa attccagcag gcaatggcgt cgcagttttt
    12181 gttgcactaa cagaagtcag ccgaatatca taagggatta atatgcgggt gaacttacgg
    12241 attgcgaatt ttggcagttt gccaacttgc gagcctgggt tgccaaacca cctgttcttg
    12301 tgcaagtgtc cattgaacac cgcgccaggg gcaccacaac ccaaccgtcg cgcgcgtacc
    12361 gaggtgtcat gggcaaagga caaacttgtt cacatgtcat ctgcgtcgtt cccgcctgag
    12421 cgacgatgat ggatgctcaa caactcggcg gcagaataaa actgccctga tcacggatat
    12481 tctctacaac cattgaatgc gcccgcatgt tcggcaaagc tcgctccaaa aaacccagcc
    12541 cacttgacgc ctatgccgac aaggtgacaa cgccagccct ggcggtcacc cacatccgca
    12601 gcggtgacca cgtctttgtc ggtagcggct gtgctgcccc gcgtgccctg ttgcaggcgc
    12661 tggaatcgat gccaggactg cccgccgatc tggaactgct ccactttttc accgtcaagg
    12721 cgtttgacca tgatgccgat ggccacagca ccacccgcct gagacaccgc actttttttg
    12781 tcagcagtga catgcgggca gcggtgaaac aaggcatggt ggagtatgtg ccgatgtcaa
    12841 ttgcgcgcgt gccggacatg atcctgctgg gccgcattcc tgtcgaagta gcgctgatcc
    12901 aggtgtcagt gcccgacgag ttcggttatg tcagcctggg ggtgtcggtt gacatcattc
    12961 ccgctgcact ggcacgggcc agactggtga ttgccgaggt caaccccaac atgccacgct
    13021 ccatggggga ctcaaccctg catgtcagcc gcatccatca cctggtgccg gtcgcaaccc
    13081 tgatcattga actgcaacgc atgccgaggc aggaccgaga cgtgcagcgg atcgcgcgtt
    13141 atatcgcggg catcattgac gatggctcga ccctgcaaat cggcatgggg caactggccc
    13201 atgaagcgct gcaatatctg acggaccgca aagacctcgg cattcattcc gatgtggtgt
    13261 ccgatgccat cattccgctg ctcgaaaatg gcaacctgac cggtgccaaa aaaacccgtc
    13321 aacctttcaa gatcgtcacc agcatggcac tgggctcacg ccgcttgtac cgcttgattg
    13381 atcgcaaccc gctgttttca ttccagccaa ttgacagcat ttgcaatctg ctgacgatct
    13441 cgaaacagca caagatggta tcgattgcac aggcttttgc gattgacctg actggccagg
    13501 tctgcatcga ccagttcgac ggcgagtttt acagcggcat cggctgccag ggtgagtttt
    13561 tgctgggtgc cgcacagtcg ccgggtggca agccaatcat ctgcatgact tcgaccaccg
    13621 acgacggact ggtttcccgc atccgtccgg cgctactgga aggcgaatca gccaccattg
    13681 cccgtagcga tgtgcattac ctggtcaccg aatacggcat cgcctacctg tttggcaagt
    13741 ccatccggca gcgcgccacc gccttgatcg agctggccca ccccaagttt cgccctgaac
    13801 tttttgccaa agcgcaagcc atgggctacc tctcggccga acaatccctg cacaacctgc
    13861 gcgcctatcc ggtagaggaa gagcaaaccg tattgctcaa ggacggccga agtgtgctgc
    13921 tgcgcccggc actctcaagc gatgcccagg gcattcgcga gttgttccac catctcagcg
    13981 aagcggacgt ttttacccgg tttttccgca atgtgcgcgg cctctctgat gccgaggtgg
    14041 aacggctgtg caacgtcaac tatgaaaacg aggtggcctt tgtcgccatc agcggtagcc
    14101 gcgaggagtc gaccattgtt ggccagtcct gttacttcat cgacccgact accaatatgg
    14161 ccgatgtggc ctacatggtg cacccgcaat ggcaggcgtg tggcctgggc agcgccatgc
    14221 aagcgtgcat gatcagacac gccacccggc ggggactgcg cggctttgtc gccgatgtgc
    14281 tgctgggcaa cgccaaaatg ctgcgcctgg cacgcaacag ctcgctgcgt gttcaggcag
    14341 aaaaaaacca ggacgcggtt cacatcacca tcctgtttta acgtgaatag ttctttggaa
    14401 ctaggccgcc acagccgggc tagttccatt ttcacggatc acacatcctt cagagggata
    14461 ccgcagacca gcccgaaaac ctaaagtcat agcctacaaa caggagtttt gctatgacca
    14521 aaagagggca agcgctctcg gtgctgatcg tcagcacctt cgcttttacg gtttgcttca
    14581 tggtgtggat gatgttcgcc gtgatcggca tcccgatcaa aaaaacactg ggcctcaacg
    14641 ccaccgagtt tggattgctc accgccatgc cggtgctgac cggctcgctg attcgggtgc
    14701 cgctgggcat ctggactgac aaatttggcg ggcgaatcgt gatggcgctg ctgatggctt
    14761 cgaccatacc agccatctgg ctgatgagtt atgccaccca atactggcat tatttgacca
    14821 ttggcctgtt tgtcggtctc gcaggtggct cgttttcagt cggcaccccc tacgtggccc
    14881 gctggttccc gaaatcgcag caaggttttg ccatgggca
//
LOCUS       DAOSLL010000028        14905 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4143_length_14905_cov_5.363367, whole genome shotgun sequence.
ACCESSION   DAOSLL010000028 DAOSLL010000000
VERSION     DAOSLL010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 14905)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14905)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14905
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4143_length_14905_cov_5.363367"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="PLB25_02110"
     CDS             <1..337
                     /locus_tag="PLB25_02110"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE40419.1"
                     /translation="LEVQLQVPGCKSYTLQSNGEIVAFGQHWVLEVPSVHLGRLIVSP
                     THRGNGFGRELCCQLIAAAKESTGASSVTLRVYKDNEVALALYHSLGLRQLAEESDGQ
                     VLFMRRNEA"
     gene            449..691
                     /locus_tag="PLB25_02115"
     CDS             449..691
                     /locus_tag="PLB25_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOE40420.1"
                     /translation="MNTLTASEARANLYRLIDQTVESHEPIMISGKRSNAVLLSAQDW
                     SAIQETLYLLAVPGMRESIMAGMAEPFAKSSKELEW"
     gene            688..951
                     /locus_tag="PLB25_02120"
     CDS             688..951
                     /locus_tag="PLB25_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HOE40421.1"
                     /translation="MSWEVVYAKQALKDAKKLAASGLKPNAQELLAVVANDPFQNPPP
                     IEKLVGDLAGAYSRRVNIQHRIVYEVFTKEKTVRVLRMWTRYE"
     gene            1197..1937
                     /locus_tag="PLB25_02125"
     CDS             1197..1937
                     /locus_tag="PLB25_02125"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE40422.1"
                     /translation="MTADILEFKVPFRRTVLVGNRWTPPAATRTALLLHGGGTSAADG
                     FVELRTFLYAKGIETLAFDCIGHGRTGGQLLGSTLHDRVLQVQAVVKSQGIVPSTLTL
                     AGFSMGAYVVVKAAVEIGVPRLCLAIPAAYAAQAYEVPFGPEFSQVLRTPRSWANSDA
                     FEITRDYTGHLLVISAQDDQIVPAEIPQRYAPISGTRASTVHHIVKRSGHNLSEHYER
                     EPQARTAAYTEIALLCQRGDAWAAHKHQ"
     gene            1947..2417
                     /locus_tag="PLB25_02130"
                     /pseudo
     CDS             1947..2417
                     /locus_tag="PLB25_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549866.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            2485..3078
                     /locus_tag="PLB25_02135"
     CDS             2485..3078
                     /locus_tag="PLB25_02135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE40423.1"
                     /translation="MNDKENLKEPAAISAIWTDSKNIGFTMASDPLTGSLLRTLAASK
                     PGGRFLELGSGTGLSAAWILDGMQAGATLTTVDNDEGLLAVLRANLTQEHRLSVVCAD
                     GDDYVRSLTAEPGKTFDFIFADTWSGKYRLLEECLSLLAPAGFYVIDDMLPQANWPEG
                     QADKASTLVAALEARKDLHITKLSWASGIIICTKHGV"
     gene            complement(3146..3361)
                     /locus_tag="PLB25_02140"
     CDS             complement(3146..3361)
                     /locus_tag="PLB25_02140"
                     /inference="COORDINATES: protein motif:HMM:NF014860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40424.1"
                     /translation="MLLKLLVDRDTDRVLGFHGVGPDTGELAQLMGVALQMQATKSAL
                     DATLAVHPTAAEELVTMRTPVMRYGFA"
     gene            complement(3262..4533)
                     /locus_tag="PLB25_02145"
     CDS             complement(3262..4533)
                     /locus_tag="PLB25_02145"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE40425.1"
                     /translation="MPEATSPFDLIVIGGGSGGVRAARIAAGHGARVALVEQHRLGGT
                     CVIRGCVPKKLMVYASRFAQEFEEAQGFGWTVGEPHFDWPTLKMRRDAEVARLEQSYR
                     QGLVSAGVAIHQGRASFVDHRTVALPDGRKFQGDHILIATGAHPVSGSDLTGHELAID
                     SDGFFELETLPRRVVVHGAGYIAVELACLLKLLGAQVDVVVRGESILRGFDADVRQHL
                     GNALADLGLKFHYGRNLAALYQSSAALRVELDDGNSLHADCVLRALGRQPNTRGLALE
                     RLLPLGLQLDAKGAIAVDAYSQTGVSGIYAVGDVTNRVNLTPMAIREGHAFADSVFGG
                     TPRSVDHGLVPTAVFTTPEAGVVGLTEAQALEQYPHLDVYRSVFRPMKSSLSASREKC
                     CSSCSLIVIPTAYWAFMAWGRTRVNWRSSWE"
     gene            complement(4579..6555)
                     /locus_tag="PLB25_02150"
     CDS             complement(4579..6555)
                     /locus_tag="PLB25_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOE40426.1"
                     /translation="MESNPIKDREELLFLLAEAAEFEHAVMCSYLYAGMSLKRSVDEG
                     VTELELAAIERWRKEIRGVALQEMLHLSLVNNMLSAFGSAPHFARPNFPIPQGRFPAA
                     IELNLSPFSESCLEHFMFIEKPLEIDMPDGAAFRHLHHYRREIRRDLFSPTPTDYASQ
                     GHLYHAIANGIDALAEKLGPDALFAGHGEAQVSGAQFALPGLFAVTDLASAHRAIEEI
                     VVQGEGAPAHSDDSHYARFRDVVAEFKQFKAARPDFEPARAAVVNPVLADPLRAKALS
                     CVTHPLAMRVVDLGNCLYTLMLRTFAQVYAPNPLPAPMRTGLAQAATAMMYALTTVGE
                     AATRLALKQDDGTHTAGLSFELPGSVGQLVQRCAAQILSERSTELANVARELEADVAL
                     PGVRDSLEALARKLEKLHAEFEGDLMPGTPLVKKPTTSTIVAAAATTATLKVDPANPN
                     AASTDAIHINYDTKRCIHSRNCVLNAPRVFLANVVGPWLHPEETTADHMAEIAQDCCS
                     GAITYQRLDGAPQEQAPEVNVLRVRENGPYAVRASIALDGQEPMFRATLCRCGKSRNK
                     PFCDSTHLEVAFTATGERTVIDSDPLVNRAGELTIEPIPNGPLEFTGNLEICGGTGHT
                     IQRVQTARLCRCGGSANKPFCDNTHARIGFRSDQ"
     gene            6986..7456
                     /locus_tag="PLB25_02155"
     CDS             6986..7456
                     /locus_tag="PLB25_02155"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOE40427.1"
                     /translation="MTPLASIRDSILALLNTIAFAVLATENAGQPHTSLIAITPFDEG
                     QRLVFATYRNTRKFTNLMQNQRVSVFMDGRNRETPISAPAGFILSAVGRVQEVSSTTY
                     PHLLSVHLQKHPDLATFTQAPDCALLEVVVDAYQVVRGIDDVTWWCLDEQKRQL"
     gene            complement(7538..7897)
                     /locus_tag="PLB25_02160"
     CDS             complement(7538..7897)
                     /locus_tag="PLB25_02160"
                     /inference="COORDINATES: protein motif:HMM:NF016705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HOE40428.1"
                     /translation="MSTIQPLPSAGQASQIQHAIQGAKDWRTHFVMHSSYTLKTLPKP
                     NNPAVKLRQVPAKTWAVLSYSGFNTEAGIQQRTNESVAWLAEKKIKSTGSPQLARYNP
                     PWTLPMFRRNEIMLEIE"
     gene            complement(7923..8309)
                     /locus_tag="PLB25_02165"
     CDS             complement(7923..8309)
                     /locus_tag="PLB25_02165"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOE40429.1"
                     /translation="MRDSGLLHGLLELETLNELLGHPVCGHSFEGHCIENLVQAAGQR
                     RVPYFYRTQVGAEIDLVLETVGKPEIAIEVKRSMAPSPEKGFAIACDDLQITQRYVVY
                     PGHERIPLRHGVQAIGLRELVDLLSQ"
     gene            complement(8897..12352)
                     /locus_tag="PLB25_02170"
     CDS             complement(8897..12352)
                     /locus_tag="PLB25_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003068131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE40430.1"
                     /translation="MKILSLRLKNLNSLKGEWFIDFRKPPFSDNGLFAITGPTGAGKS
                     TLLDAICLALYHETPRLKSISAAANDIMTRHTVDCLAEVAFEVKGAVYRAFWSQRRAR
                     DKVGGALQAPKVELAKDDGTILSSQSNDKLKRIAEITGLDFPRFTKSMLLAQGGFAAF
                     LNASANDRAELLEELTGTEIYGDISKKVFEQAREAKQQLDQLKARADGVELLTVEARD
                     GKQADVDRWGVELAGVQLAQQTTLAQRQWQHDLAQSQQEVQTNEAACLDAATAIDLAA
                     PELQRLIESEPAQALKPLHDRWQQAQATCKASDSLQNKLLQDHQRETTAHYQQQHLAQ
                     SLASQIAQNAQGQLKLTEHAHKTLTDFCAAHPQHAALGERLGSWRQQFEQRQKLQQSL
                     VAQQLALKKLQDEQAKRSRTITAQRALVDTAALADTTAASAHQLAQAEQELRLAGQTP
                     AALRQQWQARQGHVHRWQQLEAEAQVRRELSVQQTALADEVQQAEHTIAEQQATVAAL
                     RAQHQVSREQVADKQKLLDQERRIQSLDTHRRALQPGAACPLCGATEHPAIAAYQALD
                     VSATAAALKQKQDELDQLQTQGQSAATTLAASQAKQTERFKAQDKVTRQIAQWQTNWA
                     AAKSLVNATPPLTGDAWQNADTLAAGQQAAAQALATLASVLQAAEAGEQTLQRAQTAA
                     TQSAQQHQAAQSQLALLSQAAKDAQQRQTALEQEGQSYQQDLTALEASLQTALSAAGG
                     YSLPADPKPWLQERAGEWQRWQQTQRQLQDLAQTITRQQAENEAAQTQAKLWCQRWAD
                     LQASAGDQAMPLPAHPPVAADALPAALTACSDSITRLTQNIASLAGQQTQVANALVQQ
                     RSTLLEAEAAWYTALQTSPFADVSAFEAALLPEADRQRLTTLKEKLQAAQQRATALLQ
                     AARAKQKRLREQALTTLPPEDIAAQLVALEAQRSSLSEHIGAQRALLANDDKLRENQQ
                     ALFDQIALQSTDADIWQRLDGLIGSAKGDKFRKFAQGLTLDHLLHLANRHLARLHGRY
                     LLRRKITGELELDIVDAWQGDTTRDTRTLSGGEGFLVSLALALALSDLVSNKTSIDSL
                     FLDEGFGSLDGDTLEVALTALDSLNASGKMIGIISHVDAMKERIPAQIRVEKGGGVGH
                     SRLVV"
     gene            complement(12349..13584)
                     /gene="sbcD"
                     /locus_tag="PLB25_02175"
     CDS             complement(12349..13584)
                     /gene="sbcD"
                     /locus_tag="PLB25_02175"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HOE40431.1"
                     /translation="MRILHTSDWHLGQHFMGKSRQAEHQCLIDWLLLQVDAHAVDAVL
                     IAGDIFDIGSPPSYARELYSQLVVRLHRAGVALLLLGGNHDSVATLGESRVLLACLST
                     TVVAALEGAADQVLVLPLLGGKGEAGCVVCAVPFVRPRDVSQSQAGQSAEEKQQSMQT
                     AIAAYYQSVHDAGRARQAALHHELGRIVPLIATGHLTTVGASSNESVREIYVGSLDAF
                     PTAAFPPVDYVALGHIHKAQKVGGLDHIRYCGSPIALGFDEARQQKEVLLVDLGAGGL
                     AAVTALPVPRFQPLVSVSGNLLELATAIQVAAAEGSADQPVWLEVTVAEDDYLSDLPA
                     RIDAMTHGLPVEVLRVRRLRGTAAASLASELSETLDELSPDDVFARRLAQEELAPDLQ
                     LALHQRYRAVVASLVEGQA"
     gene            13642..13779
                     /locus_tag="PLB25_02180"
     CDS             13642..13779
                     /locus_tag="PLB25_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40432.1"
                     /translation="MPLKEPPIALRDDRHLRHDPDGMPAPTTPAGRAEPQSVYPQGFS
                     L"
     gene            14545..>14905
                     /locus_tag="PLB25_02185"
     CDS             14545..>14905
                     /locus_tag="PLB25_02185"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HOE40433.1"
                     /translation="MDWNPPDAWHRMADFLKNLAATLPRPPKAIVMVSAHWLEPHFAV
                     TQGAQPKLIYDYYGFPPHTYELKYPAPGQPALADQIVKMLNAAGIASASNAQRGFDHG
                     MFIPLMLIFPAANVPVVQ"
BASE COUNT         2966 a         4455 c         4385 g         3099 t
ORIGIN      
        1 gttggaagtc caacttcaag tgcccggctg caagagctac accctccaaa gcaacggtga
       61 aatcgtggct tttggtcagc actgggtcct cgaagtgcca tcggtccatc tcggcaggct
      121 tatcgtctca cccactcatc gcggcaatgg gttcgggcgt gaactttgct gtcaactcat
      181 tgccgcagcc aaggaaagca cgggcgccag ttccgttact ctgcgcgtgt acaaggacaa
      241 tgaagttgcc ttggccctgt accactcact tgggctccgg caactagcag aggaatctga
      301 tggtcaagtt ctgttcatga ggcgaaatga agcctaaccc tgcgctcgcg gctcagctca
      361 aacgttgggc ctcttgccac attcgcacag ccacttgaca agcgtacgtt atagcgtacc
      421 atttctagct tctctgcagg agcgcgaaat gaacaccctt accgccagcg aagctcgggc
      481 aaatctttac cggctcattg atcaaacagt cgagtctcac gagccaatca tgatttccgg
      541 gaaacgcagt aatgccgtgc ttctctctgc gcaagactgg agcgcaattc aagaaacttt
      601 gtacctgctg gccgtacctg gtatgcgcga gtccatcatg gccgggatgg ccgaaccctt
      661 cgcaaaaagc tcgaaggagc ttgagtggtg agttgggaag ttgtttatgc caaacaagca
      721 ttaaaggatg caaagaagct tgcggcaagc ggccttaaac caaatgcgca agaactgctc
      781 gcggttgttg ccaacgatcc atttcagaat ccacctccca ttgaaaaatt ggtcggtgat
      841 cttgccgggg catactctcg ccgcgtcaat attcagcacc gtatcgtgta tgaagtcttt
      901 accaaagaga agacggttcg cgtcctgcgc atgtggacgc gctatgaatg agaggcccgc
      961 cccatcaatt caccggacgc tgcgcaataa agccgcgcat cgccggtgaa ttcagacgct
     1021 cggcccagca agacccacac catgaaccga tcattcgcaa tttcaaatcc aatcccccga
     1081 ttgatgccca gaaggcttgg tcgtccaccg cgctggcatt ctttcaaccg ccagcattgg
     1141 ggcatgccgc attgcgccgg ttcactcgca agccaggcat cacaaagcag ccgatgttga
     1201 ccgcagatat tcttgaattc aaagtgccgt tccggcgtac cgtacttgtc ggcaaccgat
     1261 ggactccacc agcggcgacg cgcaccgccc tgctgctcca cggtggggga acttcagccg
     1321 ccgacggatt cgtcgagctc cgtacgtttc tttacgcaaa aggcatagag acgctcgcct
     1381 ttgattgcat cggccacggt cgcacgggag gccagttgct cggcagcact ctgcatgatc
     1441 gtgttctgca ggtacaggcg gtggtcaagt ctcaaggcat cgtgccatcg actctcacgc
     1501 tggctggctt cagcatgggt gcctacgtgg tggtgaaggc ggcagtggag atcggggtgc
     1561 cccgtctgtg ccttgccatt cccgccgcgt acgccgcgca ggcctacgag gttccgtttg
     1621 ggcctgagtt cagtcaggta cttcgtacgc cgcgcagttg ggcgaactcg gatgcctttg
     1681 aaatcacccg cgactacact ggccacctgc ttgtcatctc agcgcaagat gaccaaattg
     1741 tgccggcaga aataccgcaa aggtatgctc cgatcagcgg cacccgggcc tccaccgtgc
     1801 atcacatcgt caagcgctcc ggccacaacc tgagcgaaca ctacgaacgc gagccccagg
     1861 caagaacggc tgcctacaca gaaattgctt tgctgtgtca acgcggtgat gcctgggctg
     1921 cacacaaaca tcaataagtt cgccacatgc gtatcgaaat cgacgatctc agccgccctc
     1981 aagttcacgc gcttttgcaa gagcacttgg acaatatgca tgaactttca ccgccggaac
     2041 aggtttttgc gcttgacctg tccaagttgc gagcacccga catcacattc tggacggtat
     2101 ggtaagacga tgacttgatt ggctgtgggg cactgaagga actatcgccc agacatggag
     2161 agatcaagtc catgcgcacc ccgaccgcgt tgcggggcaa aggtgccggt tgtgcagtgc
     2221 tggtccacat catcaacatc gcacgtgaac gcggctattc gaagctcagc ctcgaaaccg
     2281 gtacacaccc cgccttcgaa ccagcacaaa acctctatcg cagacgagga ttcaaggaat
     2341 cgggtccgtt cgcgagctac cgcccaaacc cacacagcat gttcatggaa ctgcaactca
     2401 gcaactgtgc cgcctagcct ctggtcaccg ataagctcga agcttcggcg gcaaaacaaa
     2461 cctcctgtcc acaacagcaa cttgatgaac gataaagaaa atctcaaaga acccgcagcc
     2521 atttccgcga tttggaccga ctccaagaac attggcttca cgatggcatc cgacccgcta
     2581 accgggtcgt tgctgcgcac attggctgca agcaagccgg gcggaaggtt tctcgaactt
     2641 ggctccggaa ctggtttgtc cgcagcttgg attcttgacg gaatgcaagc cggcgcaaca
     2701 ctcacaacgg tggacaacga tgaaggcctg ctagcagtat taagggcaaa tctgacgcaa
     2761 gagcatcgtc taagtgttgt ctgcgcagac ggcgacgact atgtgcgctc actgacagcg
     2821 gaacctggca aaacctttga cttcatcttc gcggacacct ggtctggaaa gtatcgattg
     2881 ctggaagaat gcctgtctct gcttgccccg gcagggttct acgtcataga cgacatgttg
     2941 ccgcaagcca actggcctga ggggcaagct gacaaagcaa gcacacttgt cgccgccctc
     3001 gaagcacgga aagatttaca catcaccaag ctgagttggg ccagcggcat catcatctgt
     3061 acgaagcatg gggtttgatt tctcagctca aacaatgggt tttttggtca tcagagagga
     3121 atatagtttt ccgtgattca tgtgcttatg caaatccgta gcgcatgacg ggtgttcgca
     3181 tggtgaccag ttcttctgcc gccgtaggat gcactgccag tgtggcatca agcgcgcttt
     3241 tggttgcttg catttgcagc gctactccca tgagctgcgc cagttcaccc gtgtccggcc
     3301 ccacgccatg aaagcccaat acgcggtcgg tatcacgatc aacgagcagc ttgagcaaca
     3361 tttttcccgg ctggccgata gcgaagactt catcggccga aagaccgagc gatacacatc
     3421 cagatgcggg tactgttcca gggcctgcgc ttccgtcaaa ccaaccacgc cagcctcggg
     3481 cgttgtaaaa actgccgtag gcaccaagcc atggtcaacg ctgcgcggcg tgccgccaaa
     3541 cacgctgtcg gcaaaggcat gcccttcgcg aatggccatc ggggtcaggt tgacccggtt
     3601 ggtcacgtcg cccacggcat agatgccact caccccggtt tgagagtaag catctaccgc
     3661 gatggcacct ttcgcatcga gctgcaaacc gagcggcagg agtcgctcca gcgccaaacc
     3721 ccgggtattg ggctggcgac ccaatgcacg cagaacgcag tctgcgtgca gactattgcc
     3781 atcgtccaac tcaacacgca aggccgccga ggactgatat agagcagcga gattgcgtcc
     3841 atagtgaaac ttcaaaccca agtctgccag cgcgttgccc aggtgctgcc gcacatcggc
     3901 atcaaacccg cgcaagatac tctcgccgcg caccaccacg tcaacctgtg cccccaacaa
     3961 cttgagcaga catgccaatt cgaccgcaat gtagccagcg ccatgcacca ccacgcgcct
     4021 tggcagagtc tcaagctcaa aaaagccatc ggaatcaatt gccagttcat gccctgtcag
     4081 gtcactgcca cttactggat gggcaccagt agctatcaat atatgatcgc cttggaattt
     4141 tcgaccgtcg ggcaacgcca ccgtgcggtg atcgacaaaa ctggcccgac cttgatgaat
     4201 ggcaaccccg gcagacacca ggccttggcg gtaggattgc tccaaccgtg cgacttcggc
     4261 atcgcggcgc atcttgaggg tgggccaatc aaaatgcggc tcgcctacgg tccagccaaa
     4321 gccctgcgct tcttcaaatt cctgcgcaaa gcgtgacgcg tagaccatca acttcttggg
     4381 cacacagccg cggatgacgc aggtaccccc cagccggtgc tgttccacca gcgcgacacg
     4441 cgcaccatgg cccgctgcga tgcgcgcggc gcgcaccccg ccggagccac cgccaatcac
     4501 aatcagatcg aaaggcgacg tagcttctgg catcacgatg caccagtcgc tgctcgtgtg
     4561 caagccaagt cattcatctt actgatcaga gcggaagccg atccgggcat gggtgttgtc
     4621 gcaaaacggc ttgttggccg atccgccgca gcggcatagt cgagctgtct gcaccctttg
     4681 gatcgtgtgt ccggtgcctc cgcagatctc caaattgccg gtgaactcca gtggtccgtt
     4741 gggaatcggc tcaatagtca actcgccagc gcggttcaca agcggatcgg agtcgatcac
     4801 ggtacgttcg ccggtggcag tgaaagcgac ttccagatgc gtgctgtcgc aaaacggctt
     4861 gttgcgcgac tttccgcagc ggcacagtgt ggccctgaac atgggctcct ggccgtcaag
     4921 ggcaatgctg gcgcgcactg catagggtcc gttctcacgc acgcgcaaaa cattcacctc
     4981 cggtgcctgc tcctggggtg caccatcgag gcgttggtag gtgatggccc cggagcaaca
     5041 gtcctgcgca atttcggcca tgtggtcagc agtcgtttct tcggggtgca gccaggggcc
     5101 aacgacattg gccagaaata cgcgcggcgc attgagcacg caattgcgtg agtgaataca
     5161 acgcttggtg tcgtagttga tatggatggc atcggtgctt gcagcgttgg gattggcggg
     5221 atcgaccttg agcgtggcag tagtggctgc tgcggcgacg atggtgctgg tggtgggctt
     5281 cttgaccagc ggtgtgcctg gcatcagatc gccctcaaat tcggcgtgca atttttcgag
     5341 tttgcgtgcc agtgcttcca gactatcgcg aacgccagga agcgcaacat cggcctccag
     5401 ctcacgggct acgttggcaa gctccgttga gcgctcgctg agaatttgcg ctgcacagcg
     5461 ttgcaccaac tgacccactg agccgggcaa ctcaaaactc agcccggcag tgtgggtgcc
     5521 atcatcttgc ttgagcgcga gccgggtagc ggcttcgccc acggtggtca gcgcatacat
     5581 cattgccgtg gcggcttgcg ccagaccggt gcgcatcggc gcaggcaacg ggttgggcgc
     5641 gtagacctgg gcgaacgtgc gcagcatcag ggtgtacagg caattgccca ggtccaccac
     5701 ccgcatggcc agggggtgcg tgacgcagga gagcgccttg gcgcgcaacg ggtctgccag
     5761 taccggattg acaactgccg ctcgggccgg ttcaaagtcg ggtcgcgccg ccttgaactg
     5821 tttgaactcc gccaccacgt cgcgaaaccg cgcatagtgc gagtcatcgc tatgcgcggg
     5881 ggcaccttcc ccctgaacca cgatttcctc aattgcgcga tgagccgaag caagatcagt
     5941 gacagcaaac aggccaggca gtgcgaactg cgcaccactg acttgtgcct ctccatggcc
     6001 agcaaaaagc gcatcaggcc cgagtttttc cgccagcgcg tcgattccat tggcaatcgc
     6061 gtggtaaagg tgcccctgag aggcgtaatc tgtaggcgtt ggtgagaaca gatcgcgacg
     6121 aatttcgcgc cgatagtggt gcaaatgcct gaacgcagca ccatcgggca tgtcgatctc
     6181 cagaggcttc tcgatgaaca tgaagtgctc aaggcaagac tcgctaaacg gggacaggtt
     6241 caactcaatg gcagccggaa aacgcccttg agggattgga aaattgggcc gtgcaaaatg
     6301 aggtgcagag ccaaaagcac tcaacatgtt gttgaccagg ctcaggtgca gcatttcctg
     6361 caaagcgaca ccacggatct ccttgcgcca gcgctcaatt gcagccagtt cgagctcggt
     6421 cacaccctca tcaacagagc gcttcaagga cataccggca tacaggtagc tgcacatcac
     6481 tgcgtgctca aattctgcgg cctcggccag caggaacagc agttcttcac ggtctttaat
     6541 ggggttggat tccaaaatgg ctcctatagg ggaaactggg acacttgcaa aaatccgagc
     6601 atatagcttt cgagctcgcc ttgcatggtg gcgagaatcg tgagcgttca ccttcgacca
     6661 tgacaaggcg agttcgccag ccaggcgatg tcgcgtctgg cccaaaccag gggaaagacg
     6721 gcagttctgg cagtgaacgc gcaatgttcc caaagccaga cgtgttcaac ctgaaagtac
     6781 cgagtgaagt tttggtttgt agtggttgcc agggacggtt caggtcaggc ggtacgctgt
     6841 cttggctgcc gaggtccatg caatagcgcc accaaaccca gcgttttatc tggctgctga
     6901 tgctgccgtg catctccggc ttgggtagac tgcaagcttt caacctcgct ggtcagcttt
     6961 tgccaaccag ccaacttggt gttcaatgac ccctctcgcc tccatcaggg acagcatcct
     7021 ggctctgctc aacaccattg cctttgccgt acttgccact gaaaacgccg ggcaacccca
     7081 caccagtttg atcgcgatta cgccatttga tgaagggcaa cggttggttt ttgccactta
     7141 ccgcaacacc cgtaaattca ccaacttgat gcaaaaccag cgggtgtcag ttttcatgga
     7201 tgggcgtaac cgtgaaactc ccataagcgc accagccggc tttattttga gtgccgtggg
     7261 gcgggtgcaa gaagtcagtt ccaccaccta cccgcacctg ctgagtgtcc acctgcaaaa
     7321 acaccccgac cttgccacgt tcacgcaagc gccggactgt gcgttgcttg aggtggtggt
     7381 tgacgcctac caggtagtgc gcggcattga tgatgtcacc tggtggtgcc tggacgaaca
     7441 gaaaaggcaa ctttgagtca gccgggggta aagaaaaagc tgatgcagaa cacgtcgcat
     7501 caactcgccg tgaccgtgga gttgaacagc cccttggtta ctcaatttcc agcatgattt
     7561 cgtttcggcg aaacattggc aaggtccacg gcgggttata gcgtgccaac tgtggactgc
     7621 cggtggactt tatttttttc tcagccagcc aggccactga ctcattggtg cgctgctgaa
     7681 tgccggcctc ggtgttaaag ccggaataac tcaataccgc ccaggttttg gcaggcactt
     7741 gacgcaactt tacggccggg ttgtttggtt tgggcagtgt cttcagggtg taggagctgt
     7801 gcatcacaaa atgcgtccgc cagtctttgg cgccttgtat ggcatgctgt atctggctgg
     7861 cctgcccggc gcttggcaac ggctggattg tggacacagc atcgaatccg gccaccagtt
     7921 cgctactgac tcagcaagtc caccagctcc ctcaacccaa tggcctgcac cccatggcgc
     7981 agggggatgc gctcatgccc tgggtacacc acgtaacgct gcgttatctg caggtcgtcg
     8041 caagcaatcg caaagccttt ttccgggctg ggcgccatgg agcgcttgac ctcaatcgca
     8101 atctcgggct tgccaacagt ttccagcacc aggtctattt cagccccgac ctgggtgcgg
     8161 taaaagtagg gcacgcggcg ctgccctgcg gcctggacca ggttctcgat gcaatggccc
     8221 tcaaagctgt ggccgcacac cgggtgacca agcagttcgt tcaatgtttc cagctccagc
     8281 aggccgtgca gcaggccgct gtcgcgcaag aagggttttc aaaatcagct ttcagcgtca
     8341 ccttgtcagc ccatggcatt gcctatctgc acgcccccac cctggtttgc cccaagccca
     8401 tccgcaaccc atacaagacg gacagcaact ggcaaacctg cacccgtgag tttttgaagt
     8461 acatccagac gcaacaggca tccttgcctg agctggtcaa gattgcgcag gtcccccggc
     8521 ctgtctggtg tgttttgagg cggactatgc cacctgccac cgcacctatg atgcccgcgc
     8581 ggcgcgccag tttggcgggc cggcggtaat gcacctgaac agtaaaacag ccctgcctga
     8641 ttttggtttt cagcaggcgg gttgatccgg ctgatcagca gccactgggt ggcgaaagcg
     8701 gtgaatcgtc atgcccaatg ctctttaatt aatagcttct tgagcatgtt ccacatgggc
     8761 tagaggctta tttctttcgc aaactcagtc aacccccacc cctgcccact gcgccagttt
     8821 ctgctgcagt tcttctttgc tgggccaata gaactggtat tccttgccgg ttcgcacgct
     8881 ggcgcaacac tctgcgctac accaccagcc gcgaatgccc gaccccgccg cctttttcga
     8941 cccggatctg cgcggggatg cgctctttca tcgcgtccac gtggctgatg atgccgatca
     9001 tcttgccgct ggcgttgagc gagtccagcg cagtcaaagc cacttcgagc gtgtcgccgt
     9061 caagtgagcc aaagccttcg tcgaggaaga gcgaatcgat cgacgttttg ttgctcacca
     9121 ggtcagacag cgccagggcc agcgccaggc tgaccaaaaa gccttcgccg cccgacaggg
     9181 tgcgggtgtc gcgggtggtg tcaccttgcc acgcgtccac gatgtcgagc tccagttcac
     9241 cggtgatttt gcggcgcagc aggtagcggc catgcaggcg cgccaggtgc cggttggcca
     9301 ggtgcagcag gtggtcgagc gtcaggccct gggcaaactt gcgaaacttg tcgcctttgg
     9361 ccgagccgat caggccgtcc agccgctgcc agatgtcggc atcggtactt tgcagggcaa
     9421 tctgatcaaa cagtgcctgc tggttttccc gaagcttgtc gtcgttggcc agcaaggcgc
     9481 gctgggcacc gatgtgttcg ctcaagctgc tgcgctgcgc ttccagcgcc accagttgcg
     9541 cggcaatgtc ttctggcggc agcgtcgtta gcgcctgttc ccgcaaccgc ttttgcttgg
     9601 cacgtgcggc ctgcagcagg gcggtggccc gctgttgcgc ggcttgcagc ttttctttca
     9661 aagtggtcag gcgctggcgg tctgcctctg gcagcagcgc ggcctcaaat gctgacacgt
     9721 cggcgaatgg gctggtctgc agggcggtgt accaggcggc ttcggcctca agcaaggtgc
     9781 tgcgttgctg caccagggcg ttggccactt gcgtctgctg cccggccagg gaggcgatgt
     9841 tttgggtcag gcgggtgatg ctgtcgctgc aagcggtgag cgctgccggt aaggcatcgg
     9901 cagccactgg cgggtgcgcg ggcagcggca tggcttgatc accggcgctg gcctgcaggt
     9961 cagcccagcg ttggcaccac agcttggcct gggtttgcgc ggcttcgttt tcggcttgct
    10021 ggcgggtgat ggtttgcgcc aagtcttgca gttggcgttg agtctgttgc cagcgttgcc
    10081 attcaccagc gcgctcttgc agccacggtt tggggtcggc tggcaagctg tagccccccg
    10141 ctgcactcag ggcggtttgc aggctggctt ccagggcggt gaggtcttgc tgataggact
    10201 ggccttcttg ctccagtgcg gtctggcgct gttgcgcgtc tttggccgct tgcgacagca
    10261 gcgcgagctg gctttgtgcc gcctgatgtt gctgggcgct ttgggtggct gcggtttggg
    10321 cgcgttgcag ggtttgctca ccagcttctg cggcttgcag gacactggcg agtgtggcca
    10381 gtgcctgcgc tgcggcctgc tggccagcgg ccagggtgtc ggcgttttgc caagcgtcgc
    10441 cggtcagcgg cggcgtggca ttgaccaggg atttggctgc ggcccagttg gtttgccact
    10501 gggctatctg ccgggtgact ttgtcctggg ctttgaaccg ctcggtttgt ttggcttggc
    10561 tggctgccaa agtggtggcg gcgctctggc cttgcgtctg caattggtca agttcatcct
    10621 gcttttgctt caaggcggcg gcggtggcgg acacgtccag cgcctggtag gccgcaatcg
    10681 ccgggtgttc tgtcgcgcca cacagcggac aggccgcgcc cggctgcaag gcgcggcggt
    10741 gggtgtccag gctctgaatg cggcgttcct ggtcgagcag cttttgtttg tctgccacct
    10801 gctccctcga aacctggtgt tgcgcccgca gcgccgccac ggtggcctgt tgttccgcga
    10861 tggtgtgttc ggcttgctgc acctcgtctg ccagcgcggt ttgctgaacg ctgagttcgc
    10921 ggcgcacttg agcttcggct tcgagttgtt gccagcggtg gacgtggccc tgccgggctt
    10981 gccactgttg ccgcaaggca gccggtgttt gcccggccag gcgaagctct tgttcggcct
    11041 gtgccaattg gtgcgcggat gccgctgtgg tatcggccag cgcagcggtg tccaccaggg
    11101 cgcgctgggc ggtgatcgtg cggctgcgct tagcttgttc gtcttgcaac ttcttcaagg
    11161 ccagttgctg ggcgaccagg ctttgttgca gtttctgccg ttgttcaaac tgctggcgcc
    11221 aactgccaag gcgttcgccc agggcggcgt gttgcgggtg cgcggcgcag aagtcggtga
    11281 gtgttttgtg cgcgtgctcg gtgagcttga gctggccctg cgcgttttgc gctatctgac
    11341 tggccaggga ttgcgccagg tgctgctgct ggtagtgcgc tgtggtctcg cgctggtggt
    11401 cttgcagcag cttgttttgc agactgtcac tggctttgca ggtggcctgc gcttgctgcc
    11461 acctgtcgtg caagggttta agcgcctgcg cgggctcgct ctcaatcagg cgttgcagtt
    11521 cgggtgctgc cagatcaatc gcggttgccg catcaaggca agcggcctcg ttggtctgca
    11581 cctcttgctg gctttgcgcc agatcatgct gccactggcg ttgcgccaag gtggtttgct
    11641 gggccaattg cacgcccgcc agctcaaccc cccagcggtc aacatcggct tgtttgccat
    11701 cgcgcgcctc caccgtcaac agctccacac cgtcggcacg ggccttgagc tggtcgagct
    11761 gctgtttggc ctcgcgcgcc tgctcaaaca cttttttgga gatgtcgccg tagatctcgg
    11821 tgccggtgag ttcctccagc agctcggccc ggtcattggc actggcgttc agaaaagccg
    11881 caaagccgcc ctgggccagc agcatggact tggtgaagcg gggaaagtcc aggccggtga
    11941 tctcggcaat gcgcttgagt ttgtcgttgc tctggctgct cagaatcgtg ccgtcgtctt
    12001 tggccagttc cactttgggg gcctgcagcg cgccgccgac cttgtcgcgg gcacggcgct
    12061 ggctccagaa ggcgcggtac acagcgcctt tgacttcaaa cgccacctcg gccaggcaat
    12121 ccacggtgtg gcgcgtcatg atgtcgttgg ctgcggccga gatgcttttc aggcgcgggg
    12181 tctcgtggta cagcgccaag caaatggcat caagcagcgt tgatttgcca gcgccagtgg
    12241 ggccggtgat ggcaaacagg ccgttgtcgg aaaacggggg ttttctgaag tcgatgaacc
    12301 actcgccctt gagcgagttg aggtttttca ggcgcaggct gaggattttc atgcttgccc
    12361 ttcaaccagg ctggcaacca ctgcgcggta acgctggtgc agggccagtt gcaggtctgg
    12421 tgccagttcc tcctgcgcca ggcggcgtgc aaaaacatcg tcggggctga gttcatcgag
    12481 cgtttcgctc agctcactgg ccaggctggc cgctgccgtg ccgcgcaggc ggcgcacccg
    12541 caagacctcg accggcagcc cgtgggtcat ggcatcgatg cgcgcgggca ggtcagacaa
    12601 atagtcatcc tcggccacag tgacttcaag ccagacgggt tggtcggccg agccttctgc
    12661 ggccgccacc tgaatggcgg tggccagctc aagcagattg ccgctgaccg aaaccagcgg
    12721 ctgaaagcgt ggcaccggca aggccgtgac ggcagcaaga ccgcccgcgc ccaggtccac
    12781 cagcaacact tctttttgct gtcgggcttc gtcaaacccc agggcaatgg gcgagccgca
    12841 gtagcggatg tggtccagcc cacccacctt ttgcgccttg tggatgtgcc ccagcgcgac
    12901 gtagtcaacc ggcggaaagg ccgccgtggg aaaggcatcg agtgagccca catagatctc
    12961 gcgcaccgac tcgttgctgc tggcacccac ggtggtgaga tgcccggtgg cgatcagcgg
    13021 cacgatgcga cccagctcgt ggtgcagcgc ggcctggcgg gcccggcctg cgtcatgcac
    13081 cgactggtaa taggcggcaa tggcggtttg catggattgc tgcttttcct cggcgctttg
    13141 cccggcctgg ctttgcgaca cgtcacgggg gcggacgaaa ggcacggcgc agacgacgca
    13201 accggcctcg cctttgccgc ccagcaaggg cagcaccagg acctgatctg cagcaccctc
    13261 cagcgccgcc accacggtgg tgctcaggca ggccagcaac acgcggctct cgcccagcgt
    13321 ggcgaccgaa tcatggttac ccccgagcag cagcagcgca acaccggcgc ggtgcaggcg
    13381 caccaccaac tggctgtaca gctcccgcgc atagctgggc ggtgagccaa tatcgaaaat
    13441 gtcgccggcg atcagcaccg cgtctaccgc gtgcgcatcg acttgaagca acagccagtc
    13501 gatcaggcat tggtgctcgg cctggcggct cttgcccatg aaatgctgac ccaggtgcca
    13561 gtcggaggtg tggaggatgc gcatggttga acagtttggt gagcgattgc cggaaggcag
    13621 ccattttgag gctgaaatca agtgccgttg aaagaaccac cgattgcgct gcgcgatgac
    13681 cgccatctga ggcatgaccc ggatggcatg cctgcaccga ccacgcccgc tggccgtgcc
    13741 gagccacaaa gcgtttaccc gcaaggattt agtctctaac aagtcaagtc tttgcaaatc
    13801 atgcgaagaa ttcaaacatt caaagtcatt cgtcgcgagg gcgcggctcc tgcaaattca
    13861 aataggtgcc gactgtttgt gtaggagtcg cgccctcgcg acgaaggccg cccaattccg
    13921 gctcgtccgg gttaggctaa gcgcttgatt gagctgatgg agaagcaccc ggcgtgggcg
    13981 gacatttgag attttatgct acattattgg tagcttctga cgcatgctcc aaaagggctg
    14041 gaggccaatt tggcatacaa gatgcggcaa atgcggctac ggcaaggctt gcgccgcagc
    14101 atggccaccc aacccactca accgtggcca acctggaccc gcccaggtcg ccgtccaagc
    14161 tcaatcagcc aggccgcaca aggtcgcaaa agtgatggat gagcaagtcc gacgcaccac
    14221 gaacaagcgg ctggactcag cctatgaacg ggcgctggtg ttgcaacatc tcgcgtattc
    14281 acagcgtgaa aacctgaaca gcaggaagca cccttgtgag ccaaattgcc gactgtattg
    14341 aacgatgcac aaatgcgcag acacgaaaaa cctggaaatg caattgcaga tataaccaag
    14401 tggtcaactg aggaatcggg cttcagccaa acgcctcaaa cattcaaatc tgaagaaagc
    14461 accaatatgc caaacgcccc ccgaaacacc agcgcaagaa tgccaacctg gttcatcccc
    14521 cacgggggcg gtccgtgctt tttcatggac tggaatccac cagacgcatg gcatcgcatg
    14581 gctgattttt tgaaaaatct ggcggccacc ctgcctcgtc cgccaaaagc cattgtgatg
    14641 gtgtcagcac actggctgga gccacatttc gctgtgaccc aaggtgcgca gccgaaactg
    14701 atttatgact actacggctt tccgcctcac acctacgaac tgaagtaccc cgcaccgggt
    14761 cagcccgcgc tggcagatca gatcgtcaag atgctcaatg ccgctggtat cgccagtgcc
    14821 agcaatgcgc aacgcggttt cgaccatggc atgttcattc cgctcatgct gatctttcca
    14881 gcagcaaacg tgccggtagt gcaac
//
LOCUS       DAOSLL010000029        14741 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4198_length_14741_cov_4.635639, whole genome shotgun sequence.
ACCESSION   DAOSLL010000029 DAOSLL010000000
VERSION     DAOSLL010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 14741)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14741)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14741
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4198_length_14741_cov_4.635639"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..1087)
                     /locus_tag="PLB25_02190"
     CDS             complement(131..1087)
                     /locus_tag="PLB25_02190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013104494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE40434.1"
                     /translation="MKTVAIIIVNWNSWELLARCLRALAKQTCQDFSVTIIDNASTVP
                     MPEHLLADYPWAKLVFNSHNTGFAAANNLAVLQHTEQTDWIALLNPDAFPEPDWLAQL
                     LAATVAHPDCAVFASRQLSAGDPSRLDGDGDTYHMSGRAWREGHSFKVPPNSPAVADV
                     FSPCAAAGLYRRSAFVEVGGFDEDFFCYFEDVDLGFRLQLAGYGCCLITTAVVHHLGS
                     STTGGRHGDFAVYHGHRNLVWTYFKNMPGILFWVFLPLHLAINLVTLVHHGRRPALIL
                     RSKRDALFGLPAMLKKRRLIQRHRRTSIRSFWRILDKRILPK"
     gene            complement(1088..2371)
                     /locus_tag="PLB25_02195"
     CDS             complement(1088..2371)
                     /locus_tag="PLB25_02195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE40435.1"
                     /translation="MTCSVACELIARGHEVHVLTAFPSQQQASDEQRFDEYDHDGIHV
                     YRFHHAYTPMAGQTSMIEIGYDNRLAGAYFKKIVAAFKPDLVHFFHLNRLGTSLIELA
                     AQAGVPAYLTPTDFWTICPTAQLVLCNGRLCAGPSFHAGNCIKHFAERTQKGLISKFA
                     KWTPTPLAGALARLTKAGLLPRYPHDGEVKAISGRLSTNIGRLNQLRKIIAPNRFMAD
                     LLLRHGVLPSRVAQSSYGIDLPTEQFARIRRSSGQALRVGFIGTLAHYKGCHVLIESF
                     KTLPTGRATLKIYGNTQAFPGYVSDLQQRAGNHPFIEFCGVFLNSKISQVMAELDVLV
                     VPSLWYENTPLVVYSAQAAGCLVLASNFPGISEVVQDEVNGLLFEAGHSEALAARLVR
                     LCNEQGLSERLLARVDRPKSTVAYVDELLDFWMTA"
     gene            complement(2428..3360)
                     /locus_tag="PLB25_02200"
     CDS             complement(2428..3360)
                     /locus_tag="PLB25_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019141902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HOE40436.1"
                     /translation="MKIVIFGGGGFIGSTIVDRLLLEGHEIRVFERPRVAAFRKFMPG
                     ERVDWLAGDLASTHDVNDAIQGVDAVLHLVSTTLPKNSNEDPIYDVQSNVVASLQMLN
                     AMVAQGVPKIIFISSGGTVYGNPVYLPIDEKHPTDPLVSYGITKLAVEKYLQLYERLH
                     GITAIVLRVANPYGERQRPETAQGAVGVFLHHALRGMPIDIWGDGSVTRDYIHVSDVA
                     EAFASAVSYNGSKRLFNISSGVGTSLNQLLSLLEDALDKPIERRYLPGRAFDVPVSVL
                     NNSLACEHLHWSPAVAMREGITRTAQWMAEEMAR"
     gene            complement(3413..5701)
                     /locus_tag="PLB25_02205"
     CDS             complement(3413..5701)
                     /locus_tag="PLB25_02205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE40437.1"
                     /translation="MIDSLSPDFADTPGRRLQQPSYQLGSEAGKAAEVSIITPYYNTE
                     ALFLETFVSLLAQSLQNWEWVIVDDGSTDPQSMARLAEVASKDLRIKVFRQPNAGPGS
                     ARNKAVKHSVGRYLFLLDSDDMVEPTYLEKCVWFLDSNPEFAFCNSYSVVFGDEEYLW
                     TTGFERGKLHLKANSGPPISVIRRDAFLDSGGFDESIRFGHEDWDFWLAMAKAGHWGH
                     TIPEYLQWYRKRGHGRFEQIMRTGDVNRNFEKLIHEKYAGLEKKFPAPTRRPLIPFET
                     LMATSRVNNHLAPNPGGRRIMFIVPWMVAGGADRVNLDLIEGLSKKGHDISVCATLTA
                     DHTWEHQFCRFASDVFVLPNFLHASDYPRFLSYLIESRSIDTVVVTGSTIGYQLLPYL
                     RSVSSGVAFIDMCHVEELHWLNGGHPRFAIGYQELLDLNVVTTRHLAQWMLGRGADES
                     RIKVMHTGVRAAHESQLAGTRDRVRAELKIPANLPVVVFAGRICEQKRPALLAEILKK
                     VSDTGLKFNALIVGDGDQRALLEAMLSQYHLTVQVQMLGSVSHQRWLDLLVASDVLLM
                     PSQYEGISIALLEAMAAGVVPVVANVGGQEEIVSPDVGVLIAHGPHELQQYADAVHHL
                     LSDSSHLKQMADQCKAMAARQFSWQGMIDAFEGIMDESHRLRVLRPRHPISPGFGYEM
                     ASLALEYKRLGDTVDWMWRAQPLSSSVSAAMVASPAEAQAIAKFAVLLSQSRLGRLVF
                     GSRFFRSLAKRLYHRMTRLGVS"
     gene            complement(6002..7138)
                     /locus_tag="PLB25_02210"
     CDS             complement(6002..7138)
                     /locus_tag="PLB25_02210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE40438.1"
                     /translation="MNPGFSVIMTSFNYGRYIGAAIESVLAQTRQDWELLIVDDCSQD
                     DSWEIVKRYPDPRIKAHRHSVNQGACAAYNRALNMARGDYIASLDSDDLFMPTKLERQ
                     GEFLASHPEVGICGTFVVEIDGDGGKMTEQTPFADWFNVAMDLNVPVNWMRENRLCHS
                     GAVVRTELHKLVGDFDNKLVYTPDWQFWVRSLVAGVRFHVLDQPLVGYRNHGANITHK
                     NTQGAVLEQADTSARFFIPWLVRQERLDLVDRIVRSYVSDCTSDDLQRKIAERLCHGG
                     VESGMALISLVRRQDAELAARDVEINRLAQGNEWLSSQREAWENAATEREQTIGRLMV
                     QMQANAEQLATALAELAARDASLAMIRQDRGMRVTNFLRGGKFF"
     gene            complement(7141..7812)
                     /locus_tag="PLB25_02215"
     CDS             complement(7141..7812)
                     /locus_tag="PLB25_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HOE40439.1"
                     /translation="MEKIKFGLFGAGGLGREVMPYAKASVSRTLGVSPDVVDVFFVET
                     WEPKQTEVNGYGLVSMENFLQMAGPRYFNVAIGDGRVREAIVNQVGGAAQALSVHAPQ
                     TVFLDCNAIGAGSLFCPNSMVTSNVSIGRFFQANIYSYVAHDCVIGDFVTFAPGVRCN
                     GRVDIHDYAYIGTNAIIRDGTPDKRRRIGVGAVIGMGAVVTKDVPDGVTVFGNPAREL
                     PRKRG"
     gene            complement(7812..8927)
                     /locus_tag="PLB25_02220"
     CDS             complement(7812..8927)
                     /locus_tag="PLB25_02220"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOE40440.1"
                     /translation="MSVDKPIYVTQPSLPALEDFVSSLEHVWASKVLTNGGPFHQQLE
                     QALCDYLGVKHLALFVNGTIALVTALQALRIAGEVITTPYSFVATAHSLLWNGIKPVF
                     VDVDPVTLNLDPAKIEAAITEHTTAIMPVHVYGNPCDVDGIQRVADRYKLKVIYDAAH
                     AFGVQTQGVSVLNHGDLSVLSFHATKVFNTFEGGAIVCPDAQTKLRIDQLKNFGFVDE
                     VTVVAAGINGKMSEINAAFGLLQLKTIDQEIARRKAVDAIYREQLSQVEGIRCLAENA
                     QTSANYGYFPIMVESAYPLSRDGLYQKLREQHIYARRYFYPLISDFPMYRAFPSANRS
                     NLTFAANAADKVLCLPIYPGLQDIDQWRVIDAIRGRS"
     gene            complement(8963..9481)
                     /locus_tag="PLB25_02225"
     CDS             complement(8963..9481)
                     /locus_tag="PLB25_02225"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOE40441.1"
                     /translation="MKLHLGCGTNRIEGWLNVDIDSPQADLHVDLRQPLPYADATVAF
                     IFNEHFLEHITRDEGLTFLRECRRVLKPSGVFRVSTPDLRWLIAQYQSGKLDEWTDVG
                     WLPQTPCMMFNEGMRLWGHQFLYDLAELTAVLRQAGFANVSQVEHSKSSHPELIGLEC
                     RPWHHELIVEAH"
     gene            complement(9478..10866)
                     /locus_tag="PLB25_02230"
     CDS             complement(9478..10866)
                     /locus_tag="PLB25_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006485118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE40442.1"
                     /translation="MYSETIANAARPGVRPTRDVAISVKNVCKAYQIYDSPRDRLKQF
                     LLPKLGRLVGKAPKHYYREFAALAGVSFEIGRGETVGIIGRNGAGKSTLLQIICGTLT
                     PTSGQVAVNGRVAALLELGSGFNSDFTGRENVRINAGVLGLTEQEIDQRMSDILAFAD
                     IGDFVDQPVKTYSSGMYVRLAFAVVVHVDADILIVDEALSVGDMYFQAKCMAHMKRLM
                     ESGVTVLFVSHDVGSVKALCSRSVYLDRGSVVAVGPTEAVVEAYYEAGVKRGQATRPV
                     AAVSARPTGEFDASELAAQRQFAARAAFHRIQNGMAELLDVRLLDGSGQPVELVEFGQ
                     TVILRMVFKGKLDLPSIGLAYHIRDKNGFDVIYSDTGIEHCHITNLGDGEVVTMDWEF
                     MMHLRQGDYSIAAMLAIPQDLSVGKVEVCDFAPLAVSFQAQCGALPIYGAVYWANKVT
                     QQRSGAFEQGRL"
     gene            complement(10856..11689)
                     /locus_tag="PLB25_02235"
     CDS             complement(10856..11689)
                     /locus_tag="PLB25_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE40443.1"
                     /translation="MNPHADQPTTLTALISGLWNNRHLIFQMARREVLGRYRGSVIGL
                     AWSFFNPVFMLLVYTFVFSVIFKSHWVGGGEEDSKTQFAVLLFVGMIVMSLFSDVLNR
                     APGLVLSNVNYVKKVVFPLEILPVIAVGAALFHSVISLAVLLAAFVLLNGYLHWTIIF
                     IPLVLFPLVILSTGLAWMLASLGVFLRDVGQTIVIVTTVLMFLSPVFYPVTAVPVAFR
                     SFILANPLTFIIEQAREVLVWGHLPNWSGLGIYTLGAVMTAWAGYAWFQKTRKGFADV
                     L"
     gene            complement(12904..13374)
                     /locus_tag="PLB25_02240"
     CDS             complement(12904..13374)
                     /locus_tag="PLB25_02240"
                     /inference="COORDINATES: protein motif:HMM:NF037889.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOE40444.1"
                     /translation="MTGRLQITRMRNRLDATFLRAPGPGSDLEFQSDFAKYLCVLVSG
                     FFENAITALILDYVERRSSPEVTLYVERQLKYWTNPNTEKIVSLLSSFDSTWGKDATT
                     HLVDARKESLNSLVSLRHKIAHGESVGTSLYQVKEYYKTVREVVEYLTDLIEPS"
     gene            complement(13371..14480)
                     /locus_tag="PLB25_02245"
     CDS             complement(13371..14480)
                     /locus_tag="PLB25_02245"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOE40445.1"
                     /translation="MPEQAHISTEEPVDDLDELNEVLPNTYSITSYGSDPPVDGLVKR
                     MDQGDIVVPLFSSEPEKGQSTVGFQREFVWTKPQSDKFVESLLLGLPVPGIFMVKEAN
                     GKHLVLDGQQRLRTLQSFYGGVLQGKEYLLEHVQEKWKGMTYKTLHADDRRRLDDSII
                     HATIIRQDEPTEDQSSVYLIFERLNSGGTFLQPQEIRVALYHGALATLLSKLNSNADW
                     RSLFGKKSARLKDLELILRFFAMHHYAPSYRRPMKDFLNRYMAANRALAKQSAEQLSA
                     LFDSTIGIVRSRIGPRAFRLDRAINAAVVDSVMVGVASRLAAGPVTQPEAFAIAYASL
                     LADGGFRLAAERSTADEENVKTRLSKAKEAFAAVP"
     gene            complement(14542..>14741)
                     /locus_tag="PLB25_02250"
     CDS             complement(14542..>14741)
                     /locus_tag="PLB25_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40446.1"
                     /translation="SRAQSIRISFNPLVATACQRRFSPTGFILRGGRCASSISVVGRK
                     IDAIRWASQAQFFASHRQKSR"
BASE COUNT         3611 a         4148 c         3787 g         3195 t
ORIGIN      
        1 gcaagacgcg ccgtttagct ggctgaacaa gcgtcttgca acgacactgt gcgcccaaat
       61 ccgccggtgc aaatagggga agttattcct gaccgcatcc taagcgccag cgccgacgct
      121 gaaaagcggc ctatttcggc aaaatccgtt tgtccagaat tcgccaaaat gatctgatcg
      181 atgtgcgacg atggcgctgg atcaatcgac gctttttcag catggcgggc aagccaaaaa
      241 gtgcatcgcg cttggaacgc aggatcaacg ctggccgtcg gccgtggtgc accaaggtaa
      301 ccagattgat ggccagatgc aggggcaaaa atacccagaa caagatgcct ggcatgttct
      361 tgaaataggt ccacaccagg ttgcggtgtc cgtgatagac cgcaaaatcg ccatggcgcc
      421 caccagtggt tgatgagccc agatggtgca ccactgcggt ggtgatcaag caacacccgt
      481 aaccagccaa ctgcagcctg aaacccaggt caacgtcttc gaaatagcag aaaaaatctt
      541 catcaaaacc gccaacttca acaaaggcgc tgcgtcgata caggccagca gcggcacacg
      601 gcgagaagac atcggcgact gccgggctgt ttggtggcac cttgaagctg tggccctcgc
      661 gccatgcacg cccgctcatg tggtaggtgt caccatcacc atccagacgc gacggatcac
      721 ccgcgctaag ctgtcggctg gcaaaaacgg cgcagtcagg atgcgcgaca gtggcggcca
      781 gcaactgggc cagccaatca ggctctggaa aagcatccgg gttgagcagg gcaatccagt
      841 cagtctgttc cgtgtgctgc agtaccgcca gattattggc agcggcaaaa ccggtgttgt
      901 gactgttgaa caccagcttg gcccatgggt agtctgccag caagtgctcc ggcatcggta
      961 cggtgctggc gttatcaatg atggtgacac tgaagtcctg gcaggtctgc ttggcaagcg
     1021 ctcgcaggca gcgagccaac agttcccacg agttccaatt gacaatgatg atggcgactg
     1081 ttttcattca ggcggtcatc cagaagtcca gcagttcatc cacataagcg acggttgatt
     1141 tcggccgatc aaccctggca agcagccgct ccgacagacc ctgctcattg cacaggcgaa
     1201 caagccgcgc ggcgagagcc tcggaatgcc cggcttcaaa caacaagcca ttgacctcgt
     1261 cttgcacaac ctcggaaata cccggaaagt ttgaagccag caccagacaa ccggcggcct
     1321 gggctgaata gaccaccagc ggggtgtttt cgtaccagag tgaagggacc accagaacat
     1381 ccagctcagc catgacctgc gagatctttg aattcaaaaa aaccccacaa aattcgatga
     1441 atggatgatt gccagcccgc tgttgcaaat cactgacata gcctggaaac gcctgggtgt
     1501 tgccatatat tttcaaggtt gcccggccag tcggcaaggt cttgaaactc tcgattaaaa
     1561 catggcagcc cttgtagtgc gccagggtac caatgaagcc aacccgcaga gcttgcccgg
     1621 acgaccgcct gatgcgggca aattgctcgg tcggcagatc aatgccatag ctcgattggg
     1681 cgacccggct gggcaaaaca ccatgccgaa gcagcaaatc ggccatgaac ctgttgggcg
     1741 ctatgatttt tctcaattga ttgagtcggc ctatgttggt ggacaggcga ccacttatgg
     1801 ccttcacctc gccgtcatgc gggtagcgcg gcaacagccc ggctttggtg agcctggcga
     1861 gcgcgcccgc cagcggggtt ggtgtccatt tggcgaactt gctgatcagt cctttttgcg
     1921 ttcgctcggc aaaatgcttg atgcagttgc ccgcatgaaa gctcgggcca gcacacaggc
     1981 gaccattgca caggacgagt tgcgctgtag ggcaaatcgt ccagaagtcg gtaggggtca
     2041 ggtaggccgg gactcccgct tgtgcggcca gttcgatcaa tgatgtaccc agacggttga
     2101 ggtggaaaaa atgcaccaga tcgggtttga atgctgctac gattttcttg aaatacgccc
     2161 cagccaggcg gttgtcgtag ccgatttcaa tcatcgacgt ttgacccgcc atgggcgtat
     2221 aggcatggtg aaagcggtag acatgaatgc cgtcatggtc atattcatcg aagcgttgtt
     2281 catcactggc ctgctgttgc gacggaaagg cggtcagcac atgaacctca tgaccacgag
     2341 caatcagttc acaggccaca gaacaagtca aaacctcagt tccagcggca tactccggca
     2401 aaaactgatg gaccgtcaac agtattttca acgcgccatt tcctcagcca tccattgcgc
     2461 ggtgcgggtg atgccctcgc gcatcgcgac cgcaggtgac caatgaagat gttcacaggc
     2521 cagtgaattg ttcaatacgc tgaccggcac atcaaacgca cggcccggaa gatagcgacg
     2581 ctcaatgggt ttgtcgagcg catcttccag caacgacagc aactggttaa gactggtgcc
     2641 aacgccagag ctgatattga acagcctttt actcccgttg tagctcacgg cgctggcaaa
     2701 tgcctcggct acatcgctga cgtgaatgta atctcgcgtc acactgccgt ctccccagat
     2761 atcaattggc atgccgcgca gtgcatggtg cagaaacacg ccaaccgcac cttgcgccgt
     2821 ctcaggtcgc tggcgctcgc cataaggatt ggccacgcgc aggacgatgg ccgtgatgcc
     2881 gtgcaggcgc tcgtataact gcaggtattt ttctaccgca agtttggtaa tgccgtagga
     2941 caccagcggg tcagttggat gcttttcatc gataggcaaa tacaccgggt ttccatagac
     3001 cgtgccacca gaagagatga atatgatctt tggcacacct tgcgccacca tcgcgttgag
     3061 catttggagg gacgcaacca cattgctctg cacgtcatag atcggatctt cgttggagtt
     3121 ttttggcaat gtagttgaca ccaggtgcag cactgcatca acgccctgaa tggcatcatt
     3181 cacatcgtgt gtgctggcaa gatcaccggc gagccagtca accctctcgc ctggcatgaa
     3241 cttgcgaaag gcggcaaccc tgggacgctc gaaaaccctg atttcatggc cttcaagcag
     3301 aagtcgatca acgatggttg acccaataaa tccgccacca ccaaaaatca caattttcat
     3361 caagaatccc gaatcgatcc accattcaca acgctcgcca gggtaatcga gtttacgaga
     3421 ctccaagtct ggtcattcga tggtatagcc ttttggccaa tgatcggaaa aaccggctgc
     3481 caaaaaccaa acgtccaagc ctcgactggc tgagcagaac agcaaatttt gcgattgcct
     3541 gggcctctgc cgggctggca accatggccg cgctgacgct gcttgacagc ggctgagcgc
     3601 gccacatcca atccacggtg tcgccaagtc gcttgtactc cagcgccagt gaggccatct
     3661 cgtacccaaa ccccggactg atcgggtgtc gaggccggag cacgcgcaag cgatgtgatt
     3721 cgtccatgat gccctcaaaa gcgtcaatca tgccctgcca ggaaaactgc cgggcagcca
     3781 ttgccttgca ctggtctgcc atctgcttca aatgggaaga atcagacaaa aggtgatgga
     3841 ccgcatcggc atactgctgg agctcgtgcg gaccgtgcgc aatcagcacg cctacatcag
     3901 ggctgacgat ttcttcctga ccacccacat tcgccacgac gggaacaacc cctgctgcca
     3961 tcgcctccag caaggcgatt gaaataccct cgtattgcga gggcatcaac aggacatccg
     4021 aggccaccag caggtccagc caccgttgat gtgacactga cccaagcatc tgaacctgga
     4081 cggtcaaatg gtattgactg agcattgcct caagcaaagc ccgttgatcc ccatcgccaa
     4141 cgatcaaggc attgaacttc aagcccgtgt cgctgacttt tttcagaatc tcagccagca
     4201 aggccggacg cttctgctcg caaatccggc cagcaaatac aaccaccggc aagttggcag
     4261 ggattttcaa ctctgccctt accctgtcac gtgtacctgc caattgggat tcatgcgctg
     4321 cacgaacgcc tgtgtgcatg accttgatgc gactttcatc ggcaccgcgc cccagcatcc
     4381 actgcgcaag atgccgggtc gtgacaacgt tgagatcaag caattcctga taaccgatgg
     4441 caaacctggg atgaccaccg tttagccaat gaagttcttc aacatggcac atgtcaataa
     4501 aggcgacacc ggacgagact gatcgcaagt agggcagcaa ctgatagcca atcgtgctgc
     4561 cagtcaccac caccgtatca atgctccggg actcaatgag atacgacagg aatctcggat
     4621 agtctgatgc gtgaaggaaa ttgggcaaca caaaaacgtc tgatgcaaag cgacaaaatt
     4681 ggtgctccca ggtatggtct gcagtcaagg ttgcacaaac gctgatgtcg tgcccctttt
     4741 ttgacaatcc ctcaatcaag tccagattca cccggtctgc accgccggca accatccacg
     4801 ggacgatgaa catgatgcgg cgaccgccag gatttggagc caaatggtta ttgactcttg
     4861 aagtcgccat caaggtttca aaaggtatga gtggtcgcct tgtgggtgcg ggaaactttt
     4921 tttccagacc ggcatacttt tcatgtatga gcttctcgaa gttgcgattg acatcgccgg
     4981 tgcgcatgat ctgttcaaaa cggccgtgac cgcgtttgcg ataccactgc aggtattctg
     5041 gaatggtatg gccccaatga cctgccttgg ccatcgccag ccagaaatcc cagtcttcat
     5101 gaccaaaccg gattgattca tcaaaaccac cagaatccaa aaaagcatcg cgacgaataa
     5161 ctgaaatggg tggcccgcta ttggccttca agtgcaattt accccgctca aatccagtcg
     5221 tccaaagata ttcctcatca ccgaaaacaa cgctgtaaga attgcagaat gcaaactctg
     5281 gatttgaatc cagaaaccag acacattttt caagataggt tggctccacc atgtcatcgc
     5341 tgtcaagcag gaatagatag cgtccaacag aatgcttgac agccttgttg cgtgccgatc
     5401 ctggtccggc atttggctgc cgaaagacct tgatgcgcag gtctttggat gccacctcgg
     5461 cgagtctggc cattgactgg gggtcagtcg aaccgtcatc gacgatcacc cattcccagt
     5521 tctgtaacga ctgcgccaaa agggacacaa atgtctccaa aaacagtgct tccgtgttgt
     5581 agtagggggt gatgatcgaa acttcggcag ctttgcccgc ttctgagcca agttggtagc
     5641 tgggttgttg caatcttcga cctggagtgt cggcaaaatc aggagacagg gaatcaatca
     5701 tttgagtgaa acaaggcttg gtggcttaat ctaatcatga cggcttatcg gttgttatcg
     5761 ccttcatcgc ccagcctttg atggaaaatc tttcactgct aggtctgcgg aaaaatagca
     5821 taggcggttg ctacgatgat gaatagaagt gatttgggtt aattcaatga ctttggaatc
     5881 tgctgacgaa agattgccga tgatgaaagc agtaagctaa aaaccggtca attaatcaac
     5941 ggcttggctt ttgaaacaag cttgcttcaa attgaaattg cttgatgtga ttgactgcat
     6001 ttcaaaaaaa ctttccgccc cgcaaaaaat tggtgactcg catacccctg tcctgacgaa
     6061 tcattgccag gctggcatcg cgtgcggcca attcagctaa ggcagttgcc aattgttctg
     6121 catttgcctg catttgaacc atcagccttc caatggtctg ttctcgctct gtagcggcgt
     6181 tttcccatgc ctcgcgctgt gaggacaacc actcgtttcc ttgggccagt cgattgattt
     6241 cgacatcccg ggccgccaac tcagcgtcct ggcggcggac aagactgatc agagccatgc
     6301 cagattcaac gccaccatgg cacagtcgct cagcaatctt gcgctgcaag tcatcagacg
     6361 tgcaatcgct gacataactt cggacgatgc gatcaaccag atcaaggcgt tcttgccgaa
     6421 ccagccaagg gatgaagaag cgtgccgaag tgtctgcctg ctccaatact gcgccctggg
     6481 tgtttttgtg tgtgatgttg gcaccatgat tgcggtaccc aacaagaggc tggtcgagca
     6541 catgaaaacg aaccccggcg accagcgatc tgacccagaa ctgccagtcc ggtgtgtaaa
     6601 ccagcttgtt gtcgaaatcg cccaccagct tgtgcagttc ggtacgcacg accgcgccgg
     6661 aatgacacaa ccggttttca cgcatccagt tcaccggcac attgagatcc atggcaacgt
     6721 taaaccagtc agcgaacggt gtttgttcgg tcatcttgcc gccatcgcca tcgatctcaa
     6781 caacaaaagt accgcagatg ccgacttccg gatgagatgc caggaattcc ccctgacgtt
     6841 caagtttggt tggcatgaac agatcgtccg agtccagact ggcgatgtag tcgcctcgcg
     6901 ccatgttcag ggctcggttg tacgccgcgc aggccccctg attgacgctg tgccgatgcg
     6961 ctttgatgcg tggatcagga taccttttga caatctccca ggaatcatcc tgtgaacaat
     7021 catcgacgat caataactcc caatcctggc gcgtctgagc caaaaccgat tcaattgccg
     7081 cgccaatgta gcgtccgtaa ttaaaggaag tcatgatgac cgagaagcct ggattcatca
     7141 ctatccgcgc tttcgaggca gttcccgcgc cgggttaccg aacacggtga caccatctgg
     7201 aacatccttg gttaccacag cacccatgcc aatgaccgcg ccaacaccga ttctgcgccg
     7261 cttgtcaggt gtcccgtctc tgatgatcgc gttggttccg atatacgcat aatcgtggat
     7321 gtcaaccctg ccattgcacc tgacaccagg ggcaaaagta acaaaatcgc caatgacaca
     7381 gtcatgtgcc acgtaagaat aaatattagc ctggaaaaat ctgccaatgc tgacattgga
     7441 ggtcaccatg gaattggggc aaaacagtga gccagcgcca atcgcattgc agtccaaaaa
     7501 aaccgtttgt ggcgcgtgaa ccgacaaggc ctgcgcggcg ccgcccacct ggttaacgat
     7561 cgcttcgcgc acacgcccat caccaattgc aacattaaag taacggggcc ccgccatttg
     7621 aagaaaattc tccatcgaca ccaggccata tccattgacc tcagtttgct tgggttccca
     7681 tgtttccaca aaaaaaacat ccaccacgtc aggtgaaaca ccaagtgtcc gggacacgct
     7741 ggctttggca tagggcatca cctctcggcc caagccacct gcgccaaaaa gaccaaactt
     7801 gattttttcc atcaactcct cccccgaatt gcgtcaataa ctcgccattg atcgatgtcc
     7861 tgaaggccag ggtaaattgg caaacacaga accttgtcgg cagcgttcgc ggcaaaggtc
     7921 aggttgctcc gatttgcgga cggaaatgcc cggtacatcg gaaaatccga gatcagcgga
     7981 taaaagtacc gtcttgcgta aatgtgctgt tcgcgcagtt tctggtacaa cccgtcgcga
     8041 ctgagtgggt aggccgactc caccattatt ggaaagtagc cgtagttggc gcttgtctgc
     8101 gcgttctctg ccaggcagcg gattccttca acctggctca attgttcgcg gtaaatcgca
     8161 tcaactgcct tgcggcgcgc aatttcctga tcaatcgttt tgagttgcag caaaccaaag
     8221 gccgcgttga tttcgctcat cttgccattg atccctgcag caaccacggt cacctcatcg
     8281 acaaaaccaa aatttttcaa ctggtcgatg cgcagcttgg tctgtgcatc cgggcagacg
     8341 atggcgccgc cctcaaaagt attgaaaact ttggtggcgt gaaaactcaa caccgacaga
     8401 tcgccatggt tgagcacgct aacaccctgc gtttgcacgc caaaagcgtg ggctgcgtca
     8461 taaatcacct tgagcttgta gcgatcagcg acgcgctgga tgccatctac atcacatggg
     8521 ttcccgtaga catgcaccgg catgatggcc gtggtgtgct ctgtaatggc agcttcaatc
     8581 tttgccgggt ccaggttcag cgtaaccgga tcgacatcga caaaaaccgg tttgatgccg
     8641 ttccaaagca aggaatgcgc ggtcgcaaca aaagaataag gcgtggtaat gacctcgcca
     8701 gcaattcgca acgcttgcaa cgccgtcacc agggcgatgg tgccgttgac aaatagcgcc
     8761 aggtgtttga ctccgaggta atcgcaaagg gcctgttcca actgttgatg aaacggaccg
     8821 ccattggtca ggactttgct agcccaaaca tgctcaagag aagaaacaaa gtcctcaagc
     8881 gcgggcaagc tcggctgcgt gacataaatg ggcttgtcga cggacattca ggtgcctcca
     8941 tccatggtgc gggtggatgc catcagtgtg cctcgacaat caactcgtga tgccacggtc
     9001 ggcattcaag accgataagt tcgggatgcg aactttttga atgctcaacc tggctgacat
     9061 ttgcaaaacc agcctgccgc aagacagcag tcagttctgc aaggtcatac agaaattgat
     9121 gtccccataa acgcatgcct tcgttgaaca tcatgcaagg tgtctgcggc agccagccaa
     9181 cgtcggtcca ctcgtcaagc ttgccacttt ggtactgcgc aatcaaccaa cgcaagtcgg
     9241 gcgtactcac cctgaaaacc cccgacggct tcagcacccg ccgacactca cgcaggaaag
     9301 tgagcccctc gtcacgtgtg atgtgctcaa gaaaatgctc gttgaagatg aatgccacgg
     9361 ttgcatctgc gtacggcaag ggttgccgaa ggtcaacgtg aaggtctgcc tggggtgaat
     9421 caatatcgac attgagccag ccctcgatcc ggtttgtgcc acaacccaaa tgcaacttca
     9481 taagcggccc tgctcaaaag cgcccgatct ctgctgtgtc accttgttcg cccagtaaac
     9541 agcaccgtaa ataggcagcg ccccacattg cgcctggaaa ctgactgcca aaggcgcaaa
     9601 gtcgcaaacc tcaaccttcc caaccgacaa gtcttgcgga atcgcaagca tggcagcaat
     9661 cgaatagtcc ccctggcgca aatgcatcat gaactcccaa tccatcgtca cgacttcacc
     9721 gtcacccagg ttggtgatgt ggcagtgctc aataccagtg tcagaataaa tcacgtcaaa
     9781 gccgttcttg tccctgatat ggtaggccaa tccaatcgaa ggcaaatcca gcttgccttt
     9841 gaagaccatt cgaaggatca ccgtctgtcc aaactcaacc agttccaccg gctggccaga
     9901 cccatccagc agcctgacat cgagcagttc ggccatgccg ttctggatgc gatgaaatgc
     9961 tgccctggca gcaaattgac gctgcgcagc gagttcgctc gcatcgaatt cccctgtcgg
    10021 tcttgccgat accgccgcca ctgggcgggt tgcctggccc cgcttgacac ctgcctcgta
    10081 ataggcctcg acgacggcct cggtcggccc aaccgcgacc accgatccgc gatccaggta
    10141 aacagatctg ctgcacaggg ccttgacgga acctacatca tggctgacga acaaaaccgt
    10201 gacacctgac tccatcagtc ttttcatgtg cgccatgcat ttggcctgaa agtacatgtc
    10261 accaacggac agcgcttcgt caacgatcag aatgtcggca tcaacgtgca cgacgacggc
    10321 aaacgccagc cgtacataca tgcccgaaga gtaggtttta actggctggt caacaaaatc
    10381 accaatatca gcaaatgcca gaatgtcgct catgcgctgg tcaatctcct gttcagtcaa
    10441 gcccaataca cccgcattga tgcgaacatt ttcacgcccg gtgaaatccg agttgaaccc
    10501 tgagcccaat tcaagcaaag ctgcaacccg cccgttgacc gcaacctgcc cgctagtcgg
    10561 cgtcaaagtg ccgcagatga tttgcagcaa ggtcgattta cccgcaccgt tgcgcccgat
    10621 gatgccaacc gtttcacccc gaccgatttc aaacgaaacg cccgcaagtg cagcgaactc
    10681 cctgtagtaa tgtttcggcg ctttacccac caagcgccca agcttcggga gcaaaaactg
    10741 cttgagccgg tcacgcggcg agtcgtatat ctgataagcc ttgcaaacat ttttcacgct
    10801 gatcgccaca tcgcgagtcg gtcggacacc gggcctggcg gcatttgcaa tcgtttcaga
    10861 gtacatcggc gaagcctttt ctggttttct ggaaccaggc gtaacccgcc caagccgtca
    10921 tgaccgcccc gagggtgtaa atgcccaaac ctgaccagtt tggcaagtgg ccccacacca
    10981 gcacctcgcg ggcctgctca atgatgaagg tcagggggtt ggccaggatg aacgaccgaa
    11041 aagccacagg caccgccgtc accgggtaaa acaccggcga aagaaacatc aacaccgtgg
    11101 taacaatgac aatggtttgt ccaacatcgc gcagaaatac gcccaacgat gccagcatcc
    11161 aggcaagccc agtgctgagt atcaccaaag gaaaaaggac cagcgggatg aaaatgatgg
    11221 tccaatgcag atacccgttc agcaacacaa aggccgcgag taacacagcc aggctgataa
    11281 cgctgtggaa caaagctgcg ccgacggcaa tgactggaag gatttccagc ggaaacacga
    11341 cttttttgac gtagttgacg tttgacagca ccagaccagg tgcgcggttc agaacgtcgc
    11401 tgaacagact catcacaatc ataccgacga aaagcagcac tgcaaattgc gttttgctgt
    11461 cttcctcccc gccaccaacc cagtgtgatt tgaaaataac cgaaaacaca aaggtgtaca
    11521 ccagcagcat gaacaccggg ttgaaaaacg accaagccag accgatgacg gaacccctgt
    11581 agcgccccaa tacctcacgt cttgccattt gaaaaatcaa atgccggttg ttccacaaac
    11641 cgctgatcag cgccgtgagg gttgtaggtt ggtcagcgtg gggattcatg aaaaatgtgg
    11701 aaagcggcgc agaggcctag ccccaaccgg ggtgaacgcc gttcttgaat tttactttgt
    11761 ctcgactagc gaacatctgc gctgacaagc ggagccgggc ctggtggttt gccgttgggc
    11821 ctcggctcaa atgggggggt ttgacgaagc cgctacgcgg cgtaaatcgc accgacagag
    11881 cgctggctga cacgatttaa gcggcacctt cactccatct attgcgatgg cgtgattccg
    11941 cagcatttcg tcaggacacc ccagcattca ccccggcacc caagctgaat tggaccagcg
    12001 ctacaaaagc cacctcaaac acatcaagct caaaggcctg cagcccaaag ccattgatgc
    12061 ctgtacgggt ttttgcatcc caacagcgtg cggttgattg cgctgctgcg tttgctggtg
    12121 ttcaagtcgt gtggcaaaac gccagccgag tcagcaactg caaccccagt cgaacgaccg
    12181 aaactgttga ttcgttgcgg cgggggtgtg atgcgcatcg tgcatcggcg catttgccgt
    12241 ctgcgcttga gtcaccgggt aacctgctga cgttgctgcc actttcttcg acacgaagcg
    12301 gacagatcgc cacgatgcca ggttcttggt gacccagtgc gaaccattcc gggttatttc
    12361 gtcatccagg tgcaccgctc gcgaagcagc tctccccagg taagaacgcg aactctgcgt
    12421 gcacaaacgc accatttacc ttgagcgtcg aaccgatggg ctttgctgtc ttgtgccagc
    12481 ttggccatca accctccagg ccttctatgt ggtttctgtt cgtcgtctcg cacgtttgca
    12541 ctcaggcttc ctccgaacca accctcacgg gtcagccctt gccgtcggct agtggttgat
    12601 attgctctca tgagtccaat tcggttctcc cacaggggac ttgcacccca tcagttcgcg
    12661 cccatgctgg gcacacacaa acggttcaga tcgtggcttc gcacgatcta actttaatcg
    12721 ttagacgtca ctgatggtgc ctagtaatac gtttcatcaa aatggataca aaatgaaatt
    12781 gatggatttt tccgtctggc taggcgcgag gtggagacgt aaccgtagct atggcaacga
    12841 cgagcaacaa cgccaggcgg caaaagacac gatttcatgt attcgttttg gtggaacgga
    12901 ctactagctc ggctcaatta agtctgtgag gtactcaact acttcgcgaa cggtcttgta
    12961 gtattcctta acttggtaca ggctcgtccc gacagattcg ccgtgtgcga tcttatgtct
    13021 aagcgacaca agactattca agctctcttt ccgggcgtct acaagatgcg tggttgcatc
    13081 cttcccccac gtggaatcga aggacgaaag cagacttacg atcttctctg tgttcgggtt
    13141 cgtccaatac ttcaattgac gctcgacgta gagcgtcact tcgggcgagc ttctgcgctc
    13201 aacgtagtca aggatgagag cagtaatggc attttcgaag aagcccgaga caaggacgca
    13261 gagatacttc gcgaaatcag attgaaactc caaatcgctg cccgggcctg gagctcgcag
    13321 aaatgtcgcg tccagccggt tccgcattcg cgtgatttgg agtcgcccgg tcatggtact
    13381 gcggcaaatg cttccttggc ctttgataga cgtgtcttca cattctcttc gtcagcggtt
    13441 gaccgctccg cagccagacg aaaccctccg tcggccaaga gggatgcata ggctatagcg
    13501 aatgcctcag gttgcgttac agggccggct gccagtcttg aagcaacacc caccatgacg
    13561 gagtccacca ctgccgcgtt tatcgcgcgg tcaaggcgaa atgctctggg cccgattctt
    13621 gaacggacaa ttccgattgt ggaatcaaac aacgcgctga gttgctccgc agattgcttc
    13681 gcaagggctc ggtttgcggc catgtagcgg ttgagaaaat ccttcatcgg gcgcctgtaa
    13741 cttggggcgt aatggtgcat ggcgaagaat cgcaggatga gttcgagatc cttcaatctg
    13801 gcgctctttt tgccaaagag cgatcgccag tcggcattgc tgttcaattt gctaagtagc
    13861 gttgcaaggg ctccatgata tagagcaaca cggatttctt gaggttgaag aaacgttccg
    13921 cccgagttca atcgttcgaa gataaggtat acgctgctct gatcctccgt cggttcatcc
    13981 tgccgaatta tcgttgcgtg aataatgctg tcgtcaaggc gtctgcggtc atcggcgtgg
    14041 agcgtcttat atgtcatgcc cttccatttt tcttgaacat gctccaagag atactctttg
    14101 ccttgaagta ccccgccgta gaatgattgg agagttctca ggcgttgctg accatcaagt
    14161 acaaggtgtt tcccgttcgc ttccttgacc ataaatattc caggcactgg taggccaagc
    14221 aacagggact ccacaaattt gtctgactgt ggtttcgtcc agacgaattc ccgctgaaaa
    14281 cccactgtcg actgaccttt ctcaggttca gaactaaaga gtggaacgac aatatcaccc
    14341 tgatccattc gcttcaccaa gccatcgacg ggtgggtccg atccatagga cgttatggag
    14401 taggtatttg gaagaacttc gtttagttcg tcgaggtcat cgactggttc ttcggtagaa
    14461 atatgagctt gttcgggcat tgcagtctcg atgtgtgcgg ggcaactgta ttgtgtctga
    14521 cagtgaacac tggcgggcgg ctcagcggga tttttggcgg tgtgacgcga agaattgtgc
    14581 ttgtgatgcc caacgaatag catctatctt gcgcccgacg acgctgatgg aagacgcaca
    14641 acgcccgccg cgcaagataa accccgttgg actgaaccgc cgctgacagg cggtagcaac
    14701 aaggggattg aaagaaatcc tgatactttg tgcgcggctc a
//
LOCUS       DAOSLL010000030        14475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4277_length_14475_cov_4.376560, whole genome shotgun sequence.
ACCESSION   DAOSLL010000030 DAOSLL010000000
VERSION     DAOSLL010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 14475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14475
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4277_length_14475_cov_4.376560"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PLB25_02255"
     CDS             <1..575
                     /locus_tag="PLB25_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE40447.1"
                     /translation="AKANAVTGVTWTAMDPPLRETMLVRGDVDAITGFTFTSLLNLEA
                     RGVKAEDVVVLPYPNYGVKLYGNVIIASPKLVKENPAAIKAFLQAFAKGAKDVMANPA
                     AAIADVKARDGIVNVELETRRLKLAIDTAINSPNARAEGFGQVNGPRLTLMASQVSDA
                     FNTKSRVNADTVWNGSFLPAKAELAILPKK"
     gene            831..1661
                     /locus_tag="PLB25_02260"
     CDS             831..1661
                     /locus_tag="PLB25_02260"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE40448.1"
                     /translation="MMETPHPPWFVDFRDVWLAYNDELLAANNFAVEAIDLKVRQGEF
                     IAIVGPSGCGKSTFMKLTTGLKMPSMGSVLIDGQPVTGPLKISGMAFQASSLLPWRST
                     MNNVLLPLEIVEPYRSNFKAKRKEYEERARKLLQKVGLGGYEDKFPWQLSGGMQQRAS
                     ICRALIHEPKMLLLDEPFGALDAFTREELWCILRDLWTEQRFNVILVTHDLRESVFLA
                     DTVYVMSKSPGRFVVRRDIDLARPRDLEITYTKEFSDIVHELRGHIGAMRQSGAVSKP
                     "
     gene            1674..2444
                     /locus_tag="PLB25_02265"
     CDS             1674..2444
                     /locus_tag="PLB25_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE40449.1"
                     /translation="MLNKKQVEIWSPWLLLVAVVVLWQILCATFDVSEFIFPSPWRIW
                     TQFWEFRDNIAGHAWRTFWVTMVGFGLAIVVGVLLGFVIGSSRLAYAAVYPLMTAFNA
                     LPKAAFVPILVVWFGIGVGPAILTAFLISFFPIMVNIATGLATLEPELEDVLRVLGAK
                     RWDVLVKVGLPRSMPYFYGSLKVAITLAFVGTTVSEMTAANEGIGYLLISAGSSMQMG
                     LAFAGLVVVGAMAMVMYELFSAIEKHTTGWAHRGSQNH"
     gene            complement(2619..3380)
                     /locus_tag="PLB25_02270"
     CDS             complement(2619..3380)
                     /locus_tag="PLB25_02270"
                     /inference="COORDINATES: protein motif:HMM:NF017551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HOE40450.1"
                     /translation="MCLIAWNWQPDSPSPLLLISNRDEFFARPTAPLQWWDNADLLAG
                     RDLQAGGTWLGISRSGRMAALTNYRDPAAMRSDAPSRGDLVSAFLQGDAQASDFLHAL
                     AVRASDYNPFNLLVFDGKRLMGLESRHARVFALAPGLGAVSNADFNTPWPKLARLKAG
                     LQHLLAQTAASDAQLLHLLQDRRIAPDHQLPATGLPLPLERALSAAFIALPDYGTRAC
                     SVLRFVPQGISFVQQSFAADGSSETRRFWADAPPN"
     gene            complement(3496..4605)
                     /locus_tag="PLB25_02275"
     CDS             complement(3496..4605)
                     /locus_tag="PLB25_02275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE40451.1"
                     /translation="MVAFRWDPCFLTGLPTVDEQHHYLVDIINQFGEHLMRPQGASQD
                     EVERLIDELKRYTIYHFDDEEALMLRTGMDARFVASHQEEHAQFLQDVIQMHASLAPD
                     KHESALGLLNFLTNWLAYHILGTDQLMVWLIKASEAGTSADDAFANYRNSRDPATATL
                     LQAMKHLVAQVSERNRTLFELNQTLEARVARRTLALSEINQRLESMAMTDVLTGLPNR
                     RQAMLVLEREWQNASAKQTTLACMMIDADGFKQVNDTCGHDAGDEVLRRLARCLCAAV
                     RNDDVVCRLGGDEFLIICINTPLDGALLGAEKVRQQVAALRVPSGNGQWIGSISVGVA
                     ASNATQLGVEALLKAADEGVYLAKARGRNVVASVQ"
     gene            complement(4669..6993)
                     /locus_tag="PLB25_02280"
     CDS             complement(4669..6993)
                     /locus_tag="PLB25_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE40452.1"
                     /translation="MKWRFNITAKFLAFLLAVGVLPLILLGGSAFEVSKRIVIEQAET
                     ENSRLLASFSSYLRLYQDQVEDLAANIAGNTNIGLALRNADERVADTFGLLDTKAQMG
                     YILNSYVRVKGLVSINIFSREGARFQVGETLDVSQVDAIQVKQLLLKATQTDTPTRWL
                     GITDNLNTHSVHKKVLTAVRAIQHFSPATGKSDVVGVLVISLNNDIMHSFLKGAPLAP
                     GAQLMELDLSGIIALHSDPGRVGQPLMPALLELVRAPAPVTQLVLDGEEVLMRVERLS
                     QQQSLLLFMTPRAQLTQKVNRLALLTFGLVALALLCILILSWYFGRTVVGPIRTVSDG
                     FRRIATNPEAQQAPLPVGAALDEISQLVQGFNDHLTALQAQHAAAEELRQAKTVAEVA
                     NVAKSRFLATMSHEIRTPMNGILGMAQMLLVPNIKPADLQQYAQTILHSGHTLLNLLN
                     DILDISKIEADKMQLEVTVTEPWQLISEVHALFSKAAQARQLQLEPSWLGPPGQHYLM
                     DAHRVRQMLNNLVGNSLKFTEHGQIRIEGRELERQGDLATLAFSVSDTGLGISRDNLE
                     LLFKPFSQADSSTTRKFGGSGLGLSIVRNLAQLMGGDVAVDSELGKGSTFCFHIKVPV
                     AAAPQVNLPEVVVPNAQRDASGPPSLFVGRVLVVEDNLVNGMVIKALLSQLGLDMKLV
                     TDGQQAVDAISGGEHADVILMDIQMPVMDGYTATQHIRAWASEHAKPRVPIIALTADA
                     FEEDRKHCFAIGMDDFLTKPISLDALRAALVKWL"
     gene            complement(7043..8023)
                     /locus_tag="PLB25_02285"
     CDS             complement(7043..8023)
                     /locus_tag="PLB25_02285"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOE40453.1"
                     /translation="MGLMRLFPTVLVSLGLWGCGQSSGPSVNTPAQPAAPASAAASKV
                     EVGLVMKTLTNPFFVEMEKGARRAEKELGVNLTVKTAAQETSIEQQIQLVEDLIAARV
                     NAIVIAPGDSQRLIPSLKKAADAGIKIVNIDNRLDPQGLQQAGMQAVPFVGVDNEAGA
                     YKAGKYLGEGITQPTQAAILEGIRSADNARQRTQGAQRGLLENKHIKVVASETANWKI
                     DEAYEVTKLMFGKYPNIKLLFAANDVMAIGAIKYLTERGNAAVKVVGYDALSEAITEI
                     RAGKMAATIDQQAAEQGYQGVALAQKLVRLEPVAPVTTIDTRLITASTAP"
     gene            8069..>8236
                     /locus_tag="PLB25_02290"
     CDS             8069..>8236
                     /locus_tag="PLB25_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family DNA-binding protein"
                     /protein_id="HOE40454.1"
                     /translation="MTTPPISDPPASQANNPLHGITLQAMLVALSGHYGWAGLGERIA
                     LRCFTHEPSVTS"
     assembly_gap    8237..8246
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8247..8348
                     /locus_tag="PLB25_02295"
     CDS             <8247..8348
                     /locus_tag="PLB25_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VF530 family DNA-binding protein"
                     /protein_id="HOE40455.1"
                     /translation="SLKFLRKTPWAREKVEGLYLYMLREIRRNPPRT"
     gene            8532..9953
                     /locus_tag="PLB25_02300"
     CDS             8532..9953
                     /locus_tag="PLB25_02300"
                     /EC_number="1.2.1.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050269 - coniferyl-aldehyde dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006081 - cellular aldehyde metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl aldehyde dehydrogenase"
                     /protein_id="HOE40456.1"
                     /translation="MNHSETPVSSALEQALALQRNAYFVHPVPTLAERKADLRALQRF
                     IREHKEALCDAISLDYGHRSRHETLLAEVFPAVDGIDHIIGQLRGWMKPQRRSVDWRN
                     FLGASNRVIAQPLGVVGVIVPWNFPLNLSLIPLSYIFAAGNRAMVKMSENSRHLAAYL
                     MAHLPSYFPPEKVQVFDETGGVGIAFSKLKFDHLLFTGSGQTGRAVMAAAAQNLCPVT
                     LELGGKAPAIICDDFPLRSAVERIMFVKLLNAGQICTTVDHAWLPAARMDEFVSLARE
                     IVAARYPQLGSPDYTSIIDQRSFVRLLAGLDDARNRGARVLQLLDGPAFDRKSRKIAP
                     HLVLDAPPDCLLLQREIFGPILPLRTFTSLDDVIASINAGPRPLAVYPFSHDAEQVQK
                     IIDRVMSGGVSVNDALFHVGQHDLPFGGVGESGMGHYHGLEGFETFSKMRPVFYQTRF
                     STLKYLWPPYGKFADGVLRFLIR"
     gene            complement(9880..10242)
                     /locus_tag="PLB25_02305"
     CDS             complement(9880..10242)
                     /locus_tag="PLB25_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40457.1"
                     /translation="MAQNQNRPIQPELPCAMLSTPAIIICKLLDCLIFSDAFMPLAFD
                     GYALAAIYYVAFVTLTGSTEQINLFAGLLMLSQPVLPAALEKCDRTVATGCRFTESGN
                     EARRQQICRKVARGTSGC"
     gene            10246..12066
                     /locus_tag="PLB25_02310"
     CDS             10246..12066
                     /locus_tag="PLB25_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HOE40458.1"
                     /translation="MPNAFNFSASPFDCLNSAEQRLVRDSIDVAYFPKGATILDLGST
                     PTHLFVIIKGYVTQLDGNEVLTTYVPDDCFDGRALVAGKVSSRFVAVEEVVAYQLAKQ
                     AVSDLIASNTTFGALLFSELGNKLHALSDRQSQHQMQSLTMARADQAFLRPVHFVDYA
                     TDVLSVVKIFRQHRTSSVLVRDDDSTIPRLGIFTATALQRAILDGRPLDQLAVGELST
                     FSLIEVRPSDQLGDVMAVMLRHRVHRVVVAEKGDIIGILEALDMFSFLANHSHLITEQ
                     IEEAPDLAGLSQAAAQITRMIALLHRGGTRVSLIAKLVQQLNARLFERAWRLIAPADL
                     VANSCLFVMGSEGRGEQLLKTDQDNGLILRDDYQAPPDLDAICQRFSAALRDFGYPDC
                     PGDIMVSNSDWRKRLSEFGQMTRLWLLQPEPDSLMKLAIFMDAHAVCGDESLLDSLRL
                     GLMRVGTDNDPMLARFASAIDTFGSSSGWWNRLLGLDSAERLNLKKEGIFPLVHGIRS
                     LALAHQIMATSTVARIEALIAADKLPADLGADLTDSLHFFMGLKLQVGLAEIDTGRPV
                     SGAIDVARLSSLDRDLLKDTLSIVKKLRILLHLRFRLDML"
     gene            12066..12725
                     /locus_tag="PLB25_02315"
     CDS             12066..12725
                     /locus_tag="PLB25_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOE40459.1"
                     /translation="MISLSSSKPWLQAVKRQWQIYRLADPTFRFMFEPSPPDEWVALD
                     CQTTGLDLVKDEIISIEAVRISGNRIMTSERLELLVRPEHGVRAESVRFHQLREQDLT
                     DGLPADEAMKRLMHFIGSRPLVGYYLEFDLAMIERAIRPLLGIGLPQPRIEVSGFYYD
                     YKLSLLPSHRQHGVPYIDLHFATIMDDLDLPQAVAHEPLNDAVRAALAFVKLRQLQGM
                     A"
     gene            12959..13279
                     /locus_tag="PLB25_02320"
     CDS             12959..13279
                     /locus_tag="PLB25_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HOE40460.1"
                     /translation="MSSAMYESMRVNPKFQELVEKRGRFAWTLATIVLVLFYGFVLMV
                     AFSPATLGKPLSEGGMLTVGVAVELFLFILFWVLSFVYVKRANGEFDNLTQEIIKQAR
                     KDNK"
     gene            13279..>14475
                     /locus_tag="PLB25_02325"
     CDS             13279..>14475
                     /locus_tag="PLB25_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005669271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HOE40461.1"
                     /translation="MRRVTHFISALGLLALCALAFAGPPVEGALEKQATNWHAIIMFC
                     IFVALSMGITYWASNRTKSTSDFYTAGGGITGRQNGLAIAGDYMSAATLLGLTALVYG
                     SGFDGYLYMLAFFAGWPVILFLMAERLRNLGRFTFSDITAFRLDEGKVRTMAAVSSLT
                     VVCFYLIAQMVGAGQLIRLLFGLDYNIAIFGVGILMMVYVTFGGMVATTWVQIIKACM
                     LLAGGTLAMMLAFSQFGFSFTTLMEKATAVHKLGFKLMTPGSLLADPVNAISLGLGLM
                     FGTAGLPHILMRFFTVTDAKEARKSVLYASGIVAYFFNVIFLMGLCAIIIVGQNPSYF
                     EGGVIGGKLIGGGNMVAMHLAQAVGGNMLLGFMAAVAFATILAVVSGLALAGASAISH
                     DLYARVI"
BASE COUNT         2786 a         4248 c         4282 g         3149 t
ORIGIN      
        1 ttgccaaggc caacgccgtg accggtgtga cctggaccgc gatggacccg ccgctgcgcg
       61 aaaccatgct ggtgcgtggc gatgtcgatg ccatcaccgg ctttaccttc acctcgttgc
      121 tgaacctgga agctcggggc gtcaaggctg aggatgtggt ggtgctgcct taccccaatt
      181 acggggtcaa actgtacggc aatgtgatca tcgcttcgcc aaaactggtc aaggagaacc
      241 cggccgccat caaggctttt ctgcaggcct ttgccaaagg ggccaaggac gtgatggcta
      301 acccggcagc ggccattgcg gatgtcaagg cgcgtgacgg cattgtcaat gtcgaactgg
      361 aaacccgccg attgaaactg gcgatcgaca cggccatcaa cagcccgaac gcgcgcgccg
      421 aaggttttgg ccaggtcaat gggccacgcc tgacgctgat ggccagtcag gtgtcggatg
      481 ccttcaacac caagagccgc gtcaatgccg ataccgtgtg gaacggcagc tttttgcccg
      541 ccaaggcgga actggcgatt ttgcccaaga agtaacgcct atcaccaagg cctgagacgc
      601 ttttaaatat atagctgctt acgcaatcag gataagggct gaagccatat ttcctgtatt
      661 ttttgcaatg atctggccag cactctgacg gggctacacc tggccgtctt ggctacggcc
      721 acggccaccg acgcggcagc ggtagccaag tcccgacgcg aatgggttga cccgcctggc
      781 tgggccagac tgctcaaaaa ggcaacgccg atcaccaact gtgagacctg atgatggaaa
      841 caccacaccc cccctggttt gttgattttc gggatgtctg gctggcttac aacgacgagt
      901 tgctggcagc caacaacttt gccgtggaag ccattgacct gaaagtacgc cagggcgaat
      961 tcattgccat tgtcgggccg tcgggctgcg gcaaatcgac cttcatgaag ctcaccaccg
     1021 ggctgaagat gccgtccatg ggcagtgtat tgattgacgg gcagccggtc accgggccac
     1081 tcaaaatttc gggcatggcg tttcaggctt cgtcgctgct gccctggcgc agcaccatga
     1141 acaacgtgct gctgccgctg gaaattgttg agccgtaccg cagcaacttc aaggccaaac
     1201 gcaaggaata cgaggagcgc gcgcgcaaac tgctgcaaaa agtcggtctg ggcggttatg
     1261 aagacaagtt tccctggcag ctttcgggcg gcatgcagca gcgtgccagc atttgccggg
     1321 cgttgataca cgagccaaag atgctgctgc tcgatgagcc ctttggtgca ctcgatgcct
     1381 tcacccggga agagttgtgg tgcattctgc gcgacctgtg gaccgagcag cggttcaacg
     1441 tgattctggt cacccacgac ctgcgcgaat cggtttttct ggccgacacg gtgtacgtga
     1501 tgagcaaaag cccgggccgc tttgtggtgc gccgcgacat tgacctggcc cgcccgcgtg
     1561 acctggagat cacctacacc aaagaattct ccgatatcgt gcatgaattg cgcggtcaca
     1621 tcggtgccat gcgccaaagt ggtgctgtca gcaagccata agcaggaggc aatatgttga
     1681 acaaaaagca agttgaaatc tggtcgccct ggctgttgct ggtggccgtt gtggtgctgt
     1741 ggcaaatatt gtgcgcaacg tttgacgtgt cggaattcat cttcccgagc ccgtggcgca
     1801 tctggacgca gttctgggag tttcgtgaca acattgccgg ccatgcctgg cgcactttct
     1861 gggtcaccat ggtcggcttt ggactggcga ttgtggtcgg cgtgctgctg ggttttgtga
     1921 ttggcagctc gcgcctggct tacgcggcgg tttacccgct gatgacagcc ttcaacgcgc
     1981 tgcccaaagc ggcttttgtg ccgattctgg tggtgtggtt tggcattggc gtggggccgg
     2041 ccatcctgac ggcgtttttg atcagttttt ttccgatcat ggtcaacatc gccaccggtc
     2101 tggcgacgct ggagcccgaa ctcgaagacg tgctgcgggt gctcggtgcc aagcgctggg
     2161 acgtgctggt caaggttggc ctgccgcgtt ccatgcccta cttttatggc tcgctcaagg
     2221 tcgccatcac gctggctttt gttggcacca cggtcagcga gatgacggct gccaacgagg
     2281 gcattggcta cctgctgatc tcggcgggct caagcatgca gatgggcctg gcttttgccg
     2341 ggctggtggt ggtgggtgcc atggcgatgg tgatgtacga gctgttcagt gcgattgaaa
     2401 agcacaccac cggctgggcc caccggggtt cgcaaaacca ctgatctggc tgcaaccttc
     2461 tgccttggcc acggcttgtc aagctcaccg atgcatcggc aaccctgtct ttcttggcca
     2521 tccggaccgg ttgcgctatg gcccgacagg ttgatggaag agcagagcgg caggctaacc
     2581 ggggccgcct cccatccggg ctgttggcag cggccaactc aattgggcgg cgcgtctgcc
     2641 cagaaccgac gggtctccga gctgccgtct gcggcaaagc tttgttgcac aaagctgatg
     2701 ccttgcggca caaaacgcaa cacgctgcaa gcccgcgtgc catagtccgg caaggcaatg
     2761 aaggcggccg acagggcgcg ctccagcggc agtggcaggc cggtggcggg caactggtgg
     2821 tctggggcaa tgcgccggtc ttgcagcaag tgcagcaact gtgcatcgct ggccgctgtt
     2881 tgcgccaaca ggtgttgcag cccggctttc aggcgcgcca gcttgggcca gggggtgttg
     2941 aaatcagcat tcgacaccgc gcccaggcca ggggcgagcg caaacaccct ggcatggcgg
     3001 ctctccagcc ccatcaggcg cttgccatca aagaccagca ggttaaacgg gttgtagtcc
     3061 gaggcccgca ccgccagcgc gtgcagaaaa tcgcttgcct gcgcatcgcc ttgcaggaac
     3121 gcgctcacca gatcgccgcg tgaaggcgca tcactgcgca ttgccgccgg gtcgcggtag
     3181 ttggtaaggg ccgccatgcg cccgctgcgg ctgatgccca gccaggtccc gccagcctgc
     3241 aggtcgcgcc cggccagcaa atcggcgttg tcccaccact gcagcggcgc ggtcgggcgg
     3301 gcaaaaaact cgtcacgatt ggagatcagc agcagcgggc tggggctgtc gggttgccag
     3361 ttccaggcga tcaggcacat cagcgggttt ccgattcaaa aagctgacca gggatcatag
     3421 aaattaaaat gtcatttagg ccgcaagccc ttttgccaca agcgctgata gctcataatt
     3481 aaatagcaaa aaagactact ggacgctggc cacgacattg cgaccacggg ctttggccag
     3541 gtaaacgcct tcgtcagcgg ctttcagcaa agcctccaca cccaattgcg ttgcattgct
     3601 agccgccacg ccgacgctga tgctgccaat ccactgcccg ttgccgcttg gaacacgcag
     3661 cgctgcgacc tgctggcgca ctttttcagc gcccagcagc gcgccgtcta gcggggtgtt
     3721 gatgcaaatg atcaaaaact cgtcgccacc cagccgacaa accacgtcgt cattgcgcac
     3781 ggccgcgcac aggcagcgtg ccaggcggcg cagcacttcg tcaccggcat catggccaca
     3841 ggtgtcgttg acttgcttga aaccatcggc atcgatcatc atgcacgcca gcgtggtttg
     3901 tttggcggat gcgttttgcc attcgcgctc cagcaccagc atcgcctggc ggcggttcgg
     3961 cagaccggtc agcacgtcgg tcatcgccat gctttcaagc cgctggttga tttctgacag
     4021 cgccagcgtg cgccgggcga cccttgcttc gagcgtctgg ttcagttcaa acaaggtccg
     4081 gttgcgctcg gacacctggg ccaccagatg cttcatggcc tgcaacagtg tcgcggtggc
     4141 cgggtcgcgg ctgtttcgat agttggcgaa ggcgtcgtcg gccgatgtac cggcttcact
     4201 ggccttgatc agccagacca tcaactggtc ggtgcccagg atgtggtagg ccagccagtt
     4261 ggtcagaaag ttcagcaagc ccagcgcgga ttcatgtttg tccggtgcca ggctggcgtg
     4321 catctggatc acatcctgca agaactgcgc atgttcctcc tggtggctgg cgacaaaacg
     4381 ggcatccatg ccggttcgca gcatcagcgc ctcttcgtcg tcaaagtggt aaatggtgta
     4441 gcgcttgagc tcatcaatca gccgctcgac ctcatcttgg ctggccccct ggggccgcat
     4501 caggtgttcg ccgaactggt tgatgatgtc caccaggtag tggtgctgct catcaaccgt
     4561 tggcaaaccg gtcaaaaaac aagggtccca acgaaacgca accatatcaa agtcttctat
     4621 tcaaagtctt ctattctcgg tcagtcggac agcgccaacg caactgcgtc aaagccattt
     4681 gaccagtgca gcacgcagcg catcgagtga aatcggcttg gtcagaaagt cgtccatgcc
     4741 aatcgcgaag cagtgcttgc ggtcttcctc gaaggcatcc gccgtcaggg caatgatggg
     4801 cacccgcggc ttggcatgtt ctgatgccca cgcccggata tgttgggtgg cggtgtagcc
     4861 gtccatcacc ggcatttgaa tgtccatcaa aatcacgtcg gcgtgttcgc cgccgctgat
     4921 cgcatcgacc gcctgctggc cgtcggtgac cagcttcatg tcgagtccaa gctggctcag
     4981 cagggctttg atcaccattc cgttgaccag gttgtcctct accaccagca cccggccaac
     5041 aaacaggctg ggcggcccgc tggcgtcccg ttgcgcattg gggacgacga cttctggcaa
     5101 gttgacctgt ggtgcggcgg caaccggcac tttgatgtgg aagcaaaacg tcgagccttt
     5161 gcccagctcg ctgtcaactg ccacatcgcc acccatcaac tgggccaggt tgcgcacaat
     5221 cgacaagccc agcccggagc cgccgaattt gcgggtggtg gaactgtcgg cctgcgagaa
     5281 cggtttaaac agcaactcaa ggttgtcccg cgagataccc aaaccggtgt cgctgaccga
     5341 aaacgccagc gttgccaggt caccctggcg ttccagctcg cggccttcga tgcggatctg
     5401 gccgtgctcg gtgaacttca ggctgttgcc caccaggttg ttgagcatct ggcgcacacg
     5461 gtgcgcatcc atcaggtaat gctggccagg cgggccaagc caactcggtt caagttgcaa
     5521 ctgccgggcc tgcgcggctt ttgaaaaaag tgcatgaacc tcgctgatga gctgccaagg
     5581 ctcggtcact gtgacttcaa gctgcatctt gtccgcttcg atttttgata tatccaaaat
     5641 atcattgagc aggttcagca aggtgtgccc ggaatgcaga atggtttgtg cgtattgctg
     5701 caagtcagcc ggtttgatat tgggtaccag cagcatttgt gccatgccca aaataccgtt
     5761 catgggggtt cgaatttcat gcgacatggt ggccagaaag cggctcttgg ccacatttgc
     5821 tacttcagcc acggttttcg cctggcgcag ttcttcggcg gcggcatgct gggcttgcaa
     5881 ggccgtcagg tggtcgttaa agccttgcac cagttgtgaa atttcatcca gcgcggcacc
     5941 gacgggcagt ggtgcctgct gggcctcggg gttggtggca atccggcgaa aaccgtctga
     6001 cacggtccga atgggcccaa ccacggtgcg cccaaaatac cacgacaaga tcaaaatgca
     6061 cagcagggcc agcgccacca gaccaaaggt gagcaacgcc aggcggttga ctttttgcgt
     6121 caactgcgcc cgcggcgtca tgaacagcag caagctttgc tgctggctca agcgctcgac
     6181 ccgcatcaaa acctcttccc catcgagcac cagttgcgtc accggtgcgg gcgcgcgcac
     6241 cagttccagc agcgctggca tcaagggttg accgactcga ccgggatcgg agtgcagcgc
     6301 aatgatgccg ctgaggtcaa gctccatcag ttgcgcaccg ggtgccagcg gcgcaccttt
     6361 tagaaagctg tgcatgatgt cgttgttcag gctgatcacc agcacgccca ccacgtcgga
     6421 cttgccggtg gccggtgaaa aatgctgaat tgcccgcact gccgtcagta ccttcttgtg
     6481 aactgaatgg gtgttgaggt tgtcggtgat gcccagccag cgggttggcg tgtcggtttg
     6541 ggtggctttg agcagcagtt gtttgacctg aatggcatcc acctggctga catcaagcgt
     6601 ttcgccaacc tgaaagcgtg ccccctcacg cgaaaaaata ttgatcgaca ccaggccttt
     6661 gacgcgcacg tagctgttca ggatgtagcc catctgggct ttggtgtcaa gcaggccaaa
     6721 ggtatcggcg acgcgttcgt cggcgttgcg cagggccagg ccaatattgg tgttgccggc
     6781 aatattggca gccaggtctt cgacctgatc ctggtaaagc cgcagataag aagagaaact
     6841 ggccaacaga cggctgtttt cggtttcggc ctgttcgatg acgatgcgct ttgaaacctc
     6901 gaatgccgag ccacccagca agatcagcgg caacacaccg actgctagca aaaacgccag
     6961 aaacttggcc gtgatgttga agcgccactt cacgggtcgg ctcagcgcat gccagggatt
     7021 cagtgcatga cccgtgttgc gatcatggcg ctgtgctggc agtgatcagc cgggtatcga
     7081 tggtggtcac aggtgccacc ggttcaaggc gaaccagctt ctgtgccagc gccacacctt
     7141 gataaccctg ctcggcggcc tgctggtcga tggttgcggc cattttcccg gcccgaatct
     7201 cggtgatggc ctcgctcagg gcgtcataac cgaccacctt gaccgccgcg ttgccgcgct
     7261 cggtgaggta tttgatcgca ccaatggcca tcacgtcgtt ggccgcaaac agcagtttga
     7321 tattggggta tttgccaaac atcagcttgg tgacctcata ggcttcgtca attttccaat
     7381 tggcggtctc ggaggcgact accttgatgt gcttgttttc cagcaagccg cgctgcgccc
     7441 cctgggtgcg ctggcgggca ttgtcggcac tgcgaatgcc ttccagaatg gcggcctgcg
     7501 tcggctgagt gatgccttcg ccgaggtact tgccggcctt gtaggcaccg gcttcgttgt
     7561 caacgccgac aaagggcacg gcttgcatgc ccgcttgctg cagcccttgc gggtccaggc
     7621 ggttgtcgat gttgacgatc ttgatgccag catcagcggc ttttttaagt gacggaatca
     7681 gtcgctgcga gtcacctggc gcgatcacga tggcgttgac ccgcgcggca atcaagtctt
     7741 ccaccagttg aatttgctgc tcgatcgagg tttcctgcgc ggcggtcttg accgtcagat
     7801 tgacccccag ctctttctcg gcacggcggg cacctttttc catttcgaca aaaaacgggt
     7861 tggtcagggt cttcatgacc agacccacct cgaccttgct ggctgcggca gaggcagggg
     7921 ctgccggttg cgcaggtgta ttgaccgatg gaccgctgga ttgcccgcat ccccaaagcc
     7981 ccaaactgac caagacggtt gggaaaagac gcataagacc catggtattt cctttgtcaa
     8041 tgtgatgtgt tcaactatag cccaagccat gaccacgccg ccaatctctg acccacccgc
     8101 cagccaggcc aacaacccac tgcacggcat caccttgcaa gccatgctgg tggcgctgtc
     8161 cgggcactac ggctgggctg ggttgggcga gcgcattgcg ctgcgttgct ttacccacga
     8221 gcccagcgtc acctcannnn nnnnnnagcc tgaagttttt gcgcaagacc ccctgggcgc
     8281 gcgaaaaggt cgaggggctc tacctgtaca tgttgcgcga gatccgccgc aacccgccgc
     8341 gcacctgacc ggttttcagc cagactgact catttttgat agctgccagc gcaatggtgt
     8401 caagggctag agcccaattt gatgcataaa cctggagcag gcggcggggc cagcgcaagt
     8461 tagagggcga acttcaatct gggcctgaca cctgttgcga cggatcaaac cggcctaaac
     8521 tgaaatctgc catgaatcac tctgaaacac ccgtctccag cgcgttggag caggcacttg
     8581 ccttgcaacg caacgcctat tttgttcacc cggtgcccac gctggccgag cgcaaggctg
     8641 acctgcgcgc gctacagcgc ttcattcgcg agcacaaaga ggcgttgtgc gatgccatca
     8701 gcctggacta cggccaccgc tcgcgccacg aaacgctgct ggccgaggtg tttcctgccg
     8761 ttgacggcat cgaccacatc atcgggcaac tgcgtggctg gatgaagccg cagcgccgct
     8821 cagtcgattg gcgcaatttt ctgggtgcca gcaaccgggt cattgcgcag ccgctgggcg
     8881 tggtcggcgt gatcgtgcca tggaactttc cgctcaacct gagcctgatt cccttgagct
     8941 acatttttgc cgctggcaat cgcgccatgg tgaagatgag cgagaactcg cggcatctgg
     9001 cggcctatct gatggcacac ctgccgagct acttcccgcc cgaaaaagtg caggtgtttg
     9061 atgaaaccgg cggtgtcggc attgcctttt caaagctcaa gttcgaccat ctgctgttca
     9121 ccggatcggg gcaaaccggt cgcgcggtga tggcggcagc ggcgcaaaac ctgtgcccgg
     9181 taaccctcga actgggtggc aaggcacccg ccatcatttg cgacgacttc ccgctgcgct
     9241 cggcggtcga gcgcatcatg tttgtcaaac tgctcaacgc cgggcaaatc tgcaccaccg
     9301 tcgatcacgc ctggcttcct gcggccagga tggacgagtt tgtcagcctg gcgcgcgaga
     9361 ttgtggcggc gcgctatccc caactgggtt cacctgacta cacctcaatc atcgaccagc
     9421 gctcgtttgt gcgtttgctg gccggccttg acgatgcgcg taaccggggt gccagggtgc
     9481 tgcagttgct cgacgggccg gcatttgatc gcaaatcgcg caaaattgcg ccgcacctgg
     9541 tgcttgacgc gccacccgac tgcctgctgc tgcagcgtga aatttttggc ccgatcctgc
     9601 cgctgcgcac cttcacgtcg ctcgacgatg tcattgccag catcaatgcc gggccgcggc
     9661 cactggcggt ctatccgttc agccacgatg ccgaacaggt gcaaaaaatc atcgaccgtg
     9721 tgatgtctgg cggtgtgtcg gtcaacgacg cactgtttca tgttggtcag cacgacctgc
     9781 cgtttggcgg tgtcggtgag tcgggcatgg ggcattacca tggtctggag ggttttgaga
     9841 ccttctccaa aatgcgcccg gtgttttacc agacgcgttt tagcaccctg aagtacctct
     9901 ggccacctta cggcaaattt gctgacggcg tgcttcgttt cctgattcgg taaaccggca
     9961 accggtcgca acggtgcggt cgcatttttc aagcgctgct ggcagcactg gctggctcaa
    10021 catcagcagc ccggcaaaaa ggttgatctg ttcagtgctg ccggtcagcg tcacaaacgc
    10081 tacataatag atagctgcca gcgcatatcc atcaaaggct aaaggcataa aagcatctga
    10141 aaaaatcagg cagtccagca gcttgcaaat aataatcgca ggcgttgaaa gcatggcgca
    10201 aggcaattcg ggctgaatcg ggcgattctg attctgtgcc atgtcatgcc aaacgccttt
    10261 aacttctcgg cctcaccctt tgattgtctg aactcggccg agcaacgctt ggtgcgcgac
    10321 agcattgatg tcgcctattt tcccaagggc gcgacgattc tggacctggg ctcaacgcct
    10381 acccacctgt ttgtcatcat caaaggctac gtcacccagc ttgacggcaa cgaagttctc
    10441 accacctatg tgcccgacga ctgctttgac ggacgcgccc tggtggctgg caaggtcagc
    10501 agccgttttg tcgcggttga ggaggtggtg gcctaccagt tggccaagca ggcggtgagc
    10561 gacctgattg cctccaacac cacctttggc gcgctgctgt tttccgagct tggcaacaag
    10621 ctgcacgccc tgtcggatcg gcaaagccag caccagatgc agtcgctgac catggcccgc
    10681 gccgatcagg cctttttgcg cccggtgcac tttgttgact acgctaccga tgttttgagc
    10741 gtggtcaaaa tattccggca gcaccggact tcaagcgtgc tggtgcgtga cgacgactca
    10801 accatccccc ggctgggcat ttttaccgct accgccttgc agcgggccat tctggacggg
    10861 cggccactcg accagttggc cgtcggtgag ctgagcactt tttcactcat cgaggtgcgt
    10921 ccgtcggatc agcttggtga tgtcatggcg gtcatgctgc ggcaccgggt gcaccgggtg
    10981 gtggtggctg aaaagggcga catcattggt atcctggaag cgctggacat gttcagcttt
    11041 ttggccaacc attcgcacct gatcaccgag cagattgaag aggcacccga tttggcgggc
    11101 ctgagccagg ctgccgcgca gatcacccgc atgattgcgt tgctgcatcg cggcggcacc
    11161 cgggtcagcc tgatcgccaa gctggtgcag cagctcaatg cccgcctgtt cgagcgcgcc
    11221 tggcggctga tcgcgccagc cgatctggtc gccaacagtt gtctgtttgt catgggcagc
    11281 gaagggcgtg gcgagcaatt gctcaagact gaccaggaca acggcctgat cctgcgcgac
    11341 gattaccagg ccccgcccga cctggacgcc atttgccagc gtttttcggc tgcgctgcgc
    11401 gactttggtt accccgactg cccgggcgac atcatggtca gcaactccga ttggcgcaag
    11461 cgcctgagcg agtttggcca gatgacgcgc ttgtggctgc tgcaacccga acccgacagc
    11521 ctgatgaagc tggccatctt catggatgcc cacgccgtat gtggcgacga gagcctgctc
    11581 gatagcctgc ggctgggcct gatgcgcgtt ggcactgaca acgacccgat gctggcgcgg
    11641 tttgccagtg ccatcgacac ctttggcagc agcagcggct ggtggaaccg gctgctggga
    11701 ctcgacagtg ctgaacggct gaatttgaaa aaagaaggca tctttccgct ggttcacggc
    11761 atccgcagcc tggcgctggc gcaccagatc atggcaacca gcacggtggc acgcatcgaa
    11821 gcgctgattg ccgccgacaa gctaccggct gatctgggtg ccgacctgac cgacagcctg
    11881 cattttttca tggggctcaa actccaggtg gggctggccg agatcgatac cggtcggccg
    11941 gtatctgggg ccattgatgt ggcacggctg agcagcctgg accgtgactt gctcaaagac
    12001 actttgagca ttgtgaaaaa gctgcggatc ttgttgcacc ttcgtttccg gctggacatg
    12061 ctctgatgat cagtttgtct tcatcgaagc cctggctgca ggcggtcaag cgccagtggc
    12121 agatctaccg cctggccgac ccgacttttc gcttcatgtt cgagccgtcg ccgcccgacg
    12181 agtgggttgc actggactgc caaaccaccg gactcgactt ggtgaaggac gaaatcatct
    12241 cgatcgaggc ggtgcgcatc agcggcaatc gcattatgac cagcgaacgc cttgaactgc
    12301 tggtacgccc cgaacacgga gtacgcgccg aaagtgtgcg ctttcaccaa ttgcgcgagc
    12361 aggatctgac cgatggactg ccagccgatg aagccatgaa gcgcctgatg cacttcattg
    12421 gcagccgtcc gctggttggc tattacctgg aatttgacct tgccatgatc gagcgtgcca
    12481 tccggccgtt gctgggcatt gggttgccgc agcccagaat cgaagtctca ggtttttact
    12541 acgactacaa gttgtcgctg ctgccatcgc accggcaaca cggcgtgccc tacatcgacc
    12601 ttcactttgc caccatcatg gatgacctgg acttgccaca ggcagtggcc cacgagcctc
    12661 tcaatgatgc ggtgcgggcg gcgctggcat ttgtcaagct gcggcaattg cagggtatgg
    12721 catgatccct gactttttgg catgttcatg ccctgacagc gggaaatcaa tgatttcttt
    12781 tgcgaatcac caaaaatacc atagggattt ctacccgtgt caaagctgca actaggccgc
    12841 ttaaatgatc gacctttgtt ccggaggtgt ctcagttagc caaaaacgaa aataaactcc
    12901 caccggtttt ctcggttgtt tcaaccgcca tttcaaattt taggaaaagg tttacgccat
    12961 gagtagtgcg atgtacgagt cgatgcgggt taatccgaag ttccaggagc tggtcgaaaa
    13021 gcgcgggcga tttgcctgga cgctggcgac catagtgctg gttttgtttt atgggtttgt
    13081 gctgatggtt gctttcagtc cggccacgct gggcaagccc ctcagtgaag ggggcatgtt
    13141 gaccgtgggg gtcgcagttg aactttttct gttcattttg ttttgggtgc tgtcatttgt
    13201 gtatgtcaag cgcgccaatg gcgaatttga caacctgacc caagagatca tcaagcaagc
    13261 ccgaaaggac aacaaataat gcgccgcgtt acccacttta tcagcgccct ggggctgctt
    13321 gccctgtgtg ccttggcctt tgccggcccg ccggttgaag gtgccctgga aaaacaggcc
    13381 accaactggc acgccatcat catgttctgc atctttgtgg cgctgagcat gggcatcact
    13441 tactgggctt ccaaccggac caagtccacc tctgactttt ataccgccgg cggcggcatc
    13501 accggacggc aaaatggtct ggcaattgct ggtgactaca tgtcggctgc caccctgctg
    13561 ggtctgaccg cgctggttta tggcagtggc tttgacggct atctctacat gttggccttt
    13621 tttgccggct ggccggtcat cctgttcctg atggctgagc ggctgcgcaa cctgggtcgt
    13681 ttcactttct ccgacatcac cgcctttcgg ctcgatgaag gcaaggtgcg gaccatggcg
    13741 gcggtttctt cgctgacggt ggtttgcttc tacctgattg cccagatggt cggtgccggc
    13801 cagttgattc gactgctgtt tggtctggac tacaacattg ccatttttgg tgttggcatt
    13861 ttgatgatgg tctatgtgac ctttggcggc atggttgcca ccacctgggt gcagatcatc
    13921 aaggcctgca tgttgctcgc cggtggcacg ctggccatga tgctggcctt cagccagttt
    13981 ggtttttcct tcaccaccct gatggaaaag gccaccgctg tgcacaagct cggcttcaag
    14041 ctgatgaccc ccggcagcct gctggctgac ccggtcaacg ccatctcgct tggcctgggc
    14101 ctgatgtttg gtacggctgg cctgccgcac atcctgatgc gcttctttac cgtgaccgat
    14161 gccaaggaag cacgcaagtc agtgctgtat gcgtcgggca tcgtcgccta cttcttcaac
    14221 gtgatctttt tgatgggact ttgcgccatc atcatcgttg gccagaatcc gtcctatttt
    14281 gaaggcggcg tgatcggcgg caagttgatt ggcggcggca acatggtcgc catgcacctg
    14341 gcgcaggcgg ttggcggcaa catgctgctg ggcttcatgg cggctgtggc ttttgccacg
    14401 attctggcgg tggtgtcagg tctggcgctg gccggtgcct cggcgatctc gcacgacctc
    14461 tatgcccgcg tcatc
//
LOCUS       DAOSLL010000031        14310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4334_length_14310_cov_4.515468, whole genome shotgun sequence.
ACCESSION   DAOSLL010000031 DAOSLL010000000
VERSION     DAOSLL010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 14310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14310
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4334_length_14310_cov_4.515468"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="PLB25_02330"
     CDS             complement(<1..316)
                     /locus_tag="PLB25_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOE40462.1"
                     /translation="MRGLDVFFATVKLPSISKVTQALIKTLNDENAGIQDISNIIAQD
                     PALVAKLLRLANSAQFGLRRGVGSIDEAIQMVGMVQVRSLSLGACLCDTFPSLPGLNQ
                     QVF"
     gene            954..1847
                     /gene="dapA"
                     /locus_tag="PLB25_02335"
     CDS             954..1847
                     /gene="dapA"
                     /locus_tag="PLB25_02335"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HOE40463.1"
                     /translation="MTFPTSPITGSIVAMVTPMLPDGSVDYPSLRKLIDWHIAEGTDC
                     IGVVGTTGESPTVSMQENCEVIRVSVEQAAGRVPIMAGCGSNCTAEAIELATFAKKVG
                     ANCQLQVVPYYNKPTQEGQFQHFKAIAQAVDLPVILYNVPGRSVADMSLETALRLAQV
                     PGIIGIKEATGNIDRAQWLIREAPKGFAIYSGDDPSAVALMLCGGHGNISVTANVAPR
                     LMHELCIAAIAGDVKRAMEIQFKLMPLHKNLFVEANPIPVKWAMARLAKCGGTLRLPM
                     TTLSTSNEAVVENALRAVGLL"
     gene            1891..2973
                     /gene="bamC"
                     /locus_tag="PLB25_02340"
     CDS             1891..2973
                     /gene="bamC"
                     /locus_tag="PLB25_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HOE40464.1"
                     /translation="MNQSSRLVLLGLTLALAACSSFETDKVDYKSAGKAPTLEVPPDL
                     TQLSRENRYAVPGAPVTASTFQLGQATSQALPTGMSAVGDVRIERAGNQRWLVIDRSA
                     DKLWEPVKDFWQESGFLLAMDQANLGIMETDWAENRAKLPQDVIRQTLGKLIDSLYST
                     GERDKFRTRLERNPNGGTDIFISHRGMVEVYSTSQKDQTVWQPRAADPELETEFLRRL
                     MVKLGVSQAQSQSLVAASAPRTGSRVTNIDGRPSVQVDDGFDRAWRRVGLSLDRTNFT
                     VEDRDRSKGIYFVRYVTPSADKSEPGFFGKLFGSAKADSALQKFRVSVVSVGSLTTVS
                     VQNADGSNATMADAQRIVTVLAEDLK"
     gene            complement(3113..3730)
                     /locus_tag="PLB25_02345"
     CDS             complement(3113..3730)
                     /locus_tag="PLB25_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40465.1"
                     /translation="MTNASSEWFTLTPVGALRAFGQRQVDDVGQHLQVLLTGDHTFDA
                     ATWTAKAGLDGPAIANLEAQGWVQRLQRPLQGPDAKLDDFLQHVIASLSGERRVVLAS
                     DGGFCLGRAGFEQDEADVVSAAAADFGEFAARQARRGWHGASRYISFHADPEFLLPSY
                     SFVPFWVDDAGYWLVIVGEPLLNSPALVELLWGIKEAGTRFAIST"
     gene            complement(3727..4674)
                     /locus_tag="PLB25_02350"
     CDS             complement(3727..4674)
                     /locus_tag="PLB25_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40466.1"
                     /translation="MKIVLVGLSPRDETVFDLFLKRFMVDWRWQGLPAKQGEKLPSAD
                     VLVIDLAAYGWAQHSERSLGQLTHAAVAAVAILLVSANDTSWAGAKAAYTDQKWVWLR
                     KPYNADLMRGALTEAVSLVKAQKKRAAPAAPRAVAVPAGATIAPKPAASIAPGSPVSV
                     GPSTTVADSARAAELPKQAVPDHDATWASAGHDFGVDELIDCLARLPADRFVLLRKLS
                     SGLQKHLPFEVRFTMQHCLIVHPADGWVANNTPMQVVMRVCRSDALAAPVSLRELGLT
                     QAEDRVHQLGMTAHDLGDFMRELAAASFPAAQFLPHVSS"
     gene            complement(4671..5252)
                     /locus_tag="PLB25_02355"
     CDS             complement(4671..5252)
                     /locus_tag="PLB25_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40467.1"
                     /translation="MSFLPGHDLARQSVRRCSRQLLRDASNVETHIARIRLAISLPGA
                     EPVQGVLADIFTSLGSDQAELKRAALALVRGRLSTHVASWFESQVDAPPLPRISPLAT
                     RWSVLSIPSADISTRARRCSADDSRAMAAQAVKAVEKGDLQGEQLFLHHCLTCHDNLA
                     FMLARHQLLKMDAALPSGWSVASRQLEHAMEAL"
     gene            complement(5313..6062)
                     /locus_tag="PLB25_02360"
     CDS             complement(5313..6062)
                     /locus_tag="PLB25_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HOE40468.1"
                     /translation="MMLNFLIAPDFSPEHFAGWHLLNTVLQRRSGIHLHLVTPASAQE
                     QAQMLTSGKADIVYANPFDATDMIRNLGYVPFARPAQRFDEMVIATSAESAFSLLEDL
                     KPGCRIAVTENRDVKLIGLRLLEPADLNESLIEWVKVDSYQAAARKTIKAEVDAAFFL
                     ADAFASLTRLTRSQLRVLVESAISDISHVLLVKPQTDIDLAAIKNAFSSIGQSAGDQD
                     LLDSLGLPKGFEAMTQEQAEFMIDLMDTLLD"
     gene            complement(6590..7084)
                     /locus_tag="PLB25_02365"
     CDS             complement(6590..7084)
                     /locus_tag="PLB25_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOE40469.1"
                     /translation="MSLTDMSLPAEPSVGSMLTYTDGSSRKVFATSSEVMFPLGRLIV
                     SRTDLAGIITHANDAFVEMSGYTREELIGAPHHILRHPDMPKLAFKSLWDDIAVGKKW
                     HGYVKNLRKDGAYYWVYATAVPNIRAGAIVGYTSVRRKPSRTRVNELIPVYKQWLDQE
                     KATV"
     gene            complement(7096..7458)
                     /locus_tag="PLB25_02370"
     CDS             complement(7096..7458)
                     /locus_tag="PLB25_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HOE40470.1"
                     /translation="MRTDMFQQSLEDLNGSTADIEASALISTDGLMIASALPQGMDED
                     RIGAMSAALLSLGERTARELARGKLERVLIQGERGYVIMSAAGPEAVLTVLAKSNAKL
                     GLIFLDIKRAAETLSKLI"
     gene            complement(7462..8190)
                     /locus_tag="PLB25_02375"
     CDS             complement(7462..8190)
                     /locus_tag="PLB25_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HOE40471.1"
                     /translation="MTTSPANLTCQSAAVDAAITSRISARAFTAQDVPRGTIEHLLEL
                     ASRAPSGTNTQPWKVYVLQGDSRDALVNKVCAAHDALRADPALASQYREEYDYYPEKW
                     VSPYIDRRRENGWGLYGLLGIGKGDKDKMHLQQQRNYRFFDAPVGLMFTIDRVMGRGS
                     LLDYGMFVQSLMIAARAHGLHTCPQAAWNGFSSIILPHIGAGVDEMLVCAMALGYADE
                     NDVVNSFITPRIPVNEFTRWLSRS"
     gene            complement(8515..9948)
                     /locus_tag="PLB25_02380"
     CDS             complement(8515..9948)
                     /locus_tag="PLB25_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HOE40472.1"
                     /translation="MSKVIAINPTQDFKERSRQVLNDAGQRRNFRGAMDFLQAKRRAQ
                     FADQDELQGLRELGAQIRRYSLAHLPRLLEQLEAKLVAQGVQVHWAQTPEEANAIALG
                     IARRVGARHIIKGKSMVSEEIGFNHAMEAAGIEAFESDMGEYIVQLAGEAPSHIIMPA
                     IHKTKQEIAQLFADEVPGVTYTEDVDALIQIGRRVLRRKFADADIGLSGVNFCVAETG
                     TLCLVENEGNGRMCTTVPRVHIAITGIEKVVEKLEHLPPLISLLTRSATGQPVTTYVN
                     MISGPRKPGEKDGPQEVHLILLDNGRTQAYADDELRATLQCIRCGACMNHCPVYARIG
                     GHAYGTTYPGPIGAIISPHMLGLDVTYPLAFASTLCGACAEVCPVKIPITDILVRLRN
                     EAQAQPDSDDATLRGSGAGRSMAVSVVWSVWAQVYSKLGLYKMASWFMSRGRALSPSE
                     QGAWTRSRTPLVPAPKRLRDLMKERQK"
     gene            complement(9945..10619)
                     /locus_tag="PLB25_02385"
     CDS             complement(9945..10619)
                     /locus_tag="PLB25_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HOE40473.1"
                     /translation="MSPRDHILARLRATTMPNIPALPDVAGWFAGHSRTEDSAQRVTR
                     LRAALMAVKTELHDVTDANWVAVLSQIAASKGLRHLLVGADSAVAAELEASAPSTLKI
                     VRYDQPVDAWQDQLFDDIDASVTLARSAIAETGSLILWPSPSEPRLMSLVPPLHFVLL
                     DVNTIHADFYSAMVAEGWSKGMPTNALLICGPSKTADIQQTLAYGAHGPRELVVLLRQ
                     SAGAAT"
     gene            complement(10616..11428)
                     /locus_tag="PLB25_02390"
     CDS             complement(10616..11428)
                     /locus_tag="PLB25_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HOE40474.1"
                     /translation="MAINTLCARKYPDKPSDIYLFATCLVDQFIPQAGLDTVQLLERE
                     GIRVHYPQAQTCCGQPAHSSGYPDEARAVALHQLNLFAQPWPVVVPSGSCGCMIRLHY
                     PHLFADDPVLHAKALELSERVFELSEFLVHVVNFSHEDLGEECTVALHTSCHARRQMG
                     SHETSAALLDTLRHVKVVEQARIDECCGFGGTFSLLHPDISEAIVTDKVTSIKDSGVQ
                     RVVSADCGCLLNIIGRSARQDEIAACQEPSLTGQHLASFLWQRTNPGTGVRP"
     gene            11662..12207
                     /locus_tag="PLB25_02395"
     CDS             11662..12207
                     /locus_tag="PLB25_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOE40475.1"
                     /translation="MMMTVEQILATQKANIETLLNLTAKAFEGVEKIVELNMSASKAA
                     LAESGENAMALLSAKDAQELLALQSGLLQPLAEKTAAYSRHLYEIATGTTSEFTKTFE
                     GQAAEAQQKIIDLIESTAKNAPAGSETAVAVMKSVVAAASNALESVQKAVKQATEVAE
                     ANFTAASNTATTAVKSATKKR"
     gene            complement(12411..13241)
                     /locus_tag="PLB25_02400"
     CDS             complement(12411..13241)
                     /locus_tag="PLB25_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOE40476.1"
                     /translation="MPPKSEAAPPLVRKPPRLGLVLGGGAARGFAHIGVIQVLEEAGV
                     HPDLVVGTSAGSLVAALYASGKSGQDLQKLALSMDEASFSDWRLPLFKPGVLRGEALA
                     RFVSANLKSRQIQDLPMPLGVVATDLRSGLAVLFQRGDTATAVRASSAIPAVFQPVSI
                     AGRDYVDGGLVSPVPVSHAKKMGADLIIAVDVSSEPDGKDAQDTFQVLMQTFAIMGKS
                     ISAFELLGADVVVKPVMTGLTSTSFSTRLEAIQFGRQAMMRQLPALRRLMDAKAKGHP
                     "
     gene            complement(13375..>14310)
                     /locus_tag="PLB25_02405"
     CDS             complement(13375..>14310)
                     /locus_tag="PLB25_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE40477.1"
                     /translation="VVFGQDKALDMLASSVKMARSGLGKGDKPIGSFLFSGPTGVGKT
                     EAAKQLAYIMGIELIRFDMSEYMEQHAVSRLIGAPPGYVGFDQGGLLTEAITKKPHCV
                     LLLDEVEKAHPAIFNVLLQVMDHGTLTDNNGRKADFRNVIIIMTTNAGAETMNKATIG
                     FTNPREAGDEMADIKRLFTPEFRNRLDAIVSFKSLDENVILRVVDKFLLQLESQLADK
                     KVEVTFTDKLRLHLAKKGFDPLMGARPMQRLIQDTIRRALADELLFGRLIDGGRLTVD
                     LDDKDESKTDVLLTITPLPKKEGKSRPEAQEVTAS"
BASE COUNT         3314 a         4175 c         3868 g         2953 t
ORIGIN      
        1 agaatacttg ctggttcaga cccggcaggc ttgggaaggt gtcgcacaga caggccccga
       61 gcgagaggct gcgcacctgg accatgccaa ccatctggat cgcctcgtca attgagccga
      121 caccgcgccg caaaccaaac tgtgcgctgt ttgccaggcg cagcagcttg gcaaccagcg
      181 ccgggtcctg ggcgatgatg ttgctgatgt cctgaatgcc agcgttctcg tcattgagcg
      241 tcttgatcag cgcctgagtg acttttgaga tggaaggcag tttgactgtg gcgaaaaaaa
      301 catcaagtcc gcgcattgct tttcctatgg ggtaggttgg gttgatcagt gacagctaac
      361 cacatttcag accactcatg ttagggcaac cagtcggcca acccgataac cattgacgaa
      421 taatgcctga acccatcgac catgtcttgc tatcgtgatg ccaatgatcg cagcgaaggc
      481 acgactgaaa cgtactccat ggggcggaga tgctggtgcc atattcgcgc tatttgagtg
      541 ttgacccgcc ctgtcgatca aatgccaaaa cttgagccag gacgggtagc tgctttgtga
      601 attggcaagt aagtcgttgc gcagaaactt catttgtcaa tggtaaggcc tgctcctgcg
      661 ataccatctg aaatgacctg gctatttttt tggcttgtcg gctcatggca catcggcatc
      721 aggcagccaa gcgtttggca tgccagcacg acccaattct ctaccccacc ttggtgaatg
      781 aatggcggtt tgccagaact tgcgggttgt gccctttgaa aacggctttt tcggaccggc
      841 ccgagcgatt tcttcaacac agtgcggcca tgcatctgtc cgctgacctg gcggcttggg
      901 cacaccacct actctcgcta aaatagtcgg ttaaaccgtt tcgagtccac aacatgacat
      961 ttccgacgag ccccatcacc ggcagcattg ttgccatggt caccccaatg ctgcctgacg
     1021 gcagtgtgga ctacccttcg ctgcgaaagc tgattgactg gcatattgcc gaaggcaccg
     1081 actgcatcgg ggtcgttggc accacaggcg aatcgcccac tgtgagcatg caggaaaact
     1141 gcgaagtgat ccgggtctcg gtcgagcaag ctgccgggcg tgtgccgatc atggcaggct
     1201 gtggctccaa ttgcacagcc gaagccattg agctggccac atttgcaaag aaagtcggag
     1261 ccaactgcca gttgcaagtg gtgccctact acaacaagcc aacccaggaa ggtcaattcc
     1321 agcacttcaa ggcaatcgcc caggcggttg acctgccagt catcctgtac aacgtgccgg
     1381 ggcgctcggt ggcggacatg agcctggaaa ccgccttgcg gctagcgcaa gtgccgggca
     1441 tcattggcat caaggaagcc accggcaaca ttgaccgggc gcagtggctg atccgtgaag
     1501 cgcccaaagg ctttgcaatc tattcaggcg atgatccaag cgccgtggca ctgatgctat
     1561 gcggcggaca tggcaatatc agcgtgacgg caaatgtcgc accccggctg atgcatgagc
     1621 tttgcattgc ggccattgcc ggtgatgtca aacgcgccat ggaaattcag ttcaagctga
     1681 tgcccttgca caagaacctg tttgtcgaag ccaacccgat tccagtcaaa tgggccatgg
     1741 cccgcctggc aaagtgtggc ggtacgctgc gcttgccgat gacaacgttg tcaacctcaa
     1801 acgaagcggt tgttgaaaat gcactgcgcg cggttggttt gctgtaagcg ccctggtttc
     1861 agaccccaat tttcacccaa aggaaacttt gtgaaccaat cctccagact tgtacttctt
     1921 ggcctgacgc tggccctggc agcatgctca tcgtttgaga ccgataaagt cgattacaag
     1981 agcgcaggca aggcaccgac acttgaagtg ccacccgatc tgacccagct ctcgcgcgaa
     2041 aaccgctatg cggtgccagg cgcaccagtt acagcatcga cctttcagtt gggacaagcg
     2101 accagccaag ccttgccaac cggcatgagc gccgtgggtg acgtgcgcat tgagcgggct
     2161 gggaaccagc gctggctggt gatcgaccga tctgctgaca aactgtggga accggtcaag
     2221 gatttctggc aggaaagcgg attcctcctg gccatggacc aggctaacct tggcatcatg
     2281 gagaccgact gggccgagaa ccgggccaag ctacctcagg atgtgattcg tcaaacactg
     2341 ggcaaactga tcgactcgct gtattccact ggcgagcgag acaagtttcg cacccggctg
     2401 gaacgcaacc ccaatggcgg caccgacatc tttatcagcc atcgtggcat ggtcgaggtt
     2461 tatagcacca gccagaaaga ccagaccgtg tggcagcccc gcgccgcaga tcccgagctt
     2521 gagaccgagt ttttgcgccg cttgatggtc aagctgggcg tgtcgcaggc gcagtcccaa
     2581 tcgctggtag ctgccagcgc accaagaacc ggttcccgcg tgaccaacat tgatggccga
     2641 ccctcggtgc aagtagatga cggattcgac cgcgcctggc gtcgggttgg cttgtcgctg
     2701 gaccgcacca atttcaccgt ggaggatcgt gatcgcagca aaggcattta ctttgtgcgt
     2761 tatgtgacac cctctgccga caaatccgag cccggcttct tcggcaagct gttcggttct
     2821 gccaaggcag acagtgcgct gcagaagttc cgggtgtccg tggtcagcgt gggtagtttg
     2881 acgacggtgt cggttcaaaa tgccgatggc tcgaatgcca ccatggcaga tgctcagcgc
     2941 atcgttaccg tgcttgccga agacctgaag tagctcaatg cgtgcttatc cggttaacgc
     3001 ccgaggtcac taccggtggc gatgaatact gcgcatcggc actacctgtt gtggtcgaac
     3061 cgaacgtttg ggtgacaacc ggataagaac ggctaaatgg tttgagactg agtcaggtgc
     3121 tgatggcaaa acgggtgccg gcttccttga tgccccacaa cagttccacc agcgccggac
     3181 tgttgagcag gggctcacca acgatgacca gccaatagcc cgcatcgtcc acccagaacg
     3241 gcacaaaaga atagctgggc agcaaaaact cgggatcggc gtggaacgag atgtatctcg
     3301 aggcaccgtg ccagccacgg cgcgcctggc gtgcggcaaa ttcgccaaaa tctgcggctg
     3361 cagcacttac aacgtcagct tcgtcctgct caaaaccagc ccgacccagg cagaaaccac
     3421 cgtccgaggc caacaccacg cgccgctcgc cggacagtga ggcaatcaca tgctgtaaga
     3481 aatcatccag tttggcatcc ggtccctgca gcggccgctg tagacgctgc acccaaccct
     3541 gagcctcaag gttggcgatg gccgggccgt caagaccagc tttggcagtc caggtggccg
     3601 cgtcaaaagt gtgatcgcca gtcagcagca cttgcaaatg ctgtccaacg tcatctacct
     3661 ggcgctgccc aaaagcgcgc aaagccccga ccggcgtcaa ggtaaaccat tctgatgatg
     3721 cattggtcat gatgaaacat ggggtaggaa ttgcgctgca ggaaaacttg ctgcggccag
     3781 ctcacgcata aaatcgccga ggtcgtgcgc ggtcatgcca agctggtgca cccgatcttc
     3841 cgcctgcgtc agaccaagct cccgcagcga caccggcgcg gccagcgcgt cgctgcggca
     3901 cacgcgcatg accacctgca tcggcgtgtt attggcgacc cagccgtccg ccggatgaac
     3961 aatcagacaa tgctgcatcg tgaagcggac ttcaaatggc aagtgtttct gaagtcctga
     4021 actgagtttg cgaagcaaca caaagcggtc tgctggcagc cgcgccaaac aatcgatcag
     4081 ttcatccaca ccgaaatcgt gcccggctga tgcccaagtt gcatcgtgat cagggaccgc
     4141 ctgtttcggc agttcagcag cgcgagcaga gtctgccaca gttgtcgacg gacctaccga
     4201 aacgggggag cctggcgcga tagaggccgc cggtttgggc gcaatggttg caccagccgg
     4261 caccgccacg gcacgcggcg ctgccggcgc agcacgtttc ttttgcgcct tgacaagtga
     4321 cacagcctca gtgagcgcgc cgcgcatcag gtccgcgtta tagggcttgc gaagccacac
     4381 ccatttttga tctgtgtaag ctgctttggc accggcccag cttgtatcgt tggccgacac
     4441 cagcagtatc gcaacagcgg caaccgccgc atgcgtcaat tgcccaagcg accgttctga
     4501 atgctgggcc cagccatagg cggcaagatc aatcaccaaa acatcggctg acggcaactt
     4561 ttcaccctgc ttggcaggca aaccctgcca gcgccaatca accatgaatc gctttaaaaa
     4621 caaatcaaat accgtctcat ctcttggcga caagccgacc aaaacaattt tcatagggct
     4681 tccatcgcgt gctcaagctg gcgactggcc actgaccagc ccgacggcag cgctgcgtcc
     4741 attttcaaca actgatgcct ggccaacata aaggccaggt tgtcatgaca agttaggcag
     4801 tgatgcaaga ataactgctc accctgcaaa tctcccttct cgaccgcctt aacggcctgt
     4861 gcggccatgg cgcgcgaatc gtctgcactg catcggcgcg cccgggtaga aatgtcagca
     4921 gatgggatcg acagcacgct ccagcgcgtt gccagggggg aaattctggg taagggcggt
     4981 gcatcaacct gcgactcaaa ccagcttgcc acatgggtgc tgaggcggcc ccgcaccagc
     5041 gccagtgccg cacgtttgag ttctgcctgg tctgacccaa gcgaggtgaa gatatctgcc
     5101 agcacgccct gcaccggttc ggcacctggc agactgatgg ccaaccggat gcgcgcgatg
     5161 tgggtttcaa cattcgaggc atcgcgcaag agttgccgcg aacagcgccg cactgattga
     5221 cgcgccagat catggccagg taaaaagctc atacgcagtg gccttaactt gaaatgactt
     5281 tcatgtccgg ggatgaatgg agaccttcaa agtcagtcaa gcaaggtatc catcaggtca
     5341 atcatgaatt cagcctgctc ttgggtcatc gcctcaaaac cctttggcaa ccccagggaa
     5401 tccagcaaat cctgatcacc ggcactctga ccgatactgg aaaaagcatt tttgatagca
     5461 gccaaatcaa tgtcggtctg cggtttgacc aataaaacat gggagatatc actgatggca
     5521 ctttccacca acacacgcaa ctggctgcgc gtcaggcggg tcagggacgc aaacgcatca
     5581 gccaaaaaaa acgctgcatc cacctccgcc ttgatggttt tgcgtgctgc tgcctgatag
     5641 ctgtccacct tgacccattc aatcagcgat tcattcagat cggcaggctc aagcaggcgc
     5701 agaccgatga gcttgacatc gcggttttcg gtcaccgcaa tgcgacagcc cggcttaagg
     5761 tcttcgagta agctgaaggc ggactcggca ctggtagcga tcaccatttc gtcgaaacgc
     5821 tgcgccggcc gcgcgaaagg aacatagccc agattgcgga tcatatccgt ggcatcgaac
     5881 ggattggcgt agacgatgtc tgccttgccg ctggtcagca tctgtgcctg ctcttgcgcg
     5941 ctggcagggg tcaccagatg cagatgaatg ccggaacgcc tttgcaacac agtattcaac
     6001 aagtgccaac cggcgaaatg ctctggcgaa aaatccggcg ctattaaaaa attaagcatc
     6061 atcctgaatt cctcaatggg acgcacccga tggaaccact ggcatcccga ttgggtaggg
     6121 cgaaagctgc ggctccaaac gaatctccgc acaattggac ggcttcaagc aggccaacct
     6181 tgtggcatgg ctgcgcccag catgaagcag caagctgcgc ggagcgtcga cgacgcccgc
     6241 tattgatcgc atgtggtcct ggcggacgag gcaggagcgg cgaccccagg cgcgctgcca
     6301 gtggttcaat cgagtgcgaa accctgtcac ccaatgggtg aaccccgatg aagctaaaaa
     6361 gcaaatgcgc atgaggactt tcagatgaaa acaagcgatc aactcacaag gcgcaaataa
     6421 tgttcacaat caaaacagta gctagaaacg cataattcac aagggataga acccgatttg
     6481 gcagataaaa gccgggcggc attgaccgcc ttcgccgtcg gcctcaatcc catgttcctt
     6541 tgaggacatc tggcccgcgt gcggggttgg gatgctgagg aatccaggat catacggtcg
     6601 ccttttcctg atccagccat tgtttgtaga ctggaatcaa ttcgttgacc cgcgtgcgcg
     6661 atggcttgcg tcgcaccgag gtatagccga cgatggcccc ggcccgaata ttgggcacgg
     6721 cagtggcata gacccaatag tacgcgccat cttttcgcaa gtttttcaca tatccgtgcc
     6781 acttcttgcc gaccgctatg tcatcccaaa ggctcttgaa ggcgagcttg ggcatgtccg
     6841 ggtgacgaag aatatggtgt ggcgcgccaa tcaattcctc acgggtgtaa ccactcatct
     6901 caacaaaggc atcattggca tgggtgatga tgcccgcgag gtcagtgcgg gagacgatca
     6961 accgtcccag cgggaacatg acctcgctgc tggttgcaaa taccttgcga gaactgccat
     7021 cggtataagt cagcatgctg cccaccgatg gctcggcggg taaggacatg tcagtgaggc
     7081 tcatgacgca ctccatcaaa tcaacttgga cagtgtctcg gcagcccgtt tgatatccag
     7141 aaagatcaga cccagtttgg cattggactt ggccagcacg gtcagaaccg cctctgggcc
     7201 agcagcactc atgatcacat aaccgcgctc accctggatc agcacgcgct caagcttgcc
     7261 acgcgcaagt tcacgtgcgg tgcgctcacc gagagacagc aaggccgccg acatggcacc
     7321 gatgcggtct tcgtccattc cctgcggcag tgccgatgca atcatcaatc cgtctgtcga
     7381 aatcagggcc gaggcctcaa tgtctgcagt tgacccattc aggtcttcca gcgactgctg
     7441 aaacatatct gtgcgcattt tctagctcct ggacaaccaa cgggtaaatt cattcacggg
     7501 tatccgtggc gttatgaatg aattcacaac atcattttca tcagcatacc ccagtgccat
     7561 cgcgcacacc agcatttcat caacacctgc gccgatatgc ggcaaaatga tgctggaaaa
     7621 tccgttccag gcggcctgag ggcaagtgtg cagcccatgc gcccgggctg caatcatcag
     7681 gctttgcaca aacatgccat agtccagcag cgaacctctt cccatgacgc ggtcaatcgt
     7741 gaacatcaac ccaaccggcg catcaaaaaa ccggtagttg cgctgctgct gcagatgcat
     7801 cttgtccttg tcgcccttgc caatgcccag caacccatac agaccccagc cattctcacg
     7861 acgacggtcg atgtagggac tgacccattt ctcggggtaa tagtcatact cttcgcgata
     7921 ctgggaggca agcgccgggt cggctcgcag ggcatcgtgg gcagcgcaaa ccttattgac
     7981 cagggcatcg cgactgtccc cctgcaacac atagactttc cagggctggg tattggtgcc
     8041 agagggagcc cgactggcaa gctccaacaa atgttcgatg gttcctcgcg gtacatcttg
     8101 cgctgtaaag gcgcgcgccg aaatgcgaga ggtaatcgcc gcgtccacag cagcagactg
     8161 acaggtcagg ttggcggggg aggttgtcat aaaagcttct ccagaatttg tttgaagttg
     8221 acgggcattg ctctgattcc atttccatac caatccgaca tgtttttgct tcgccctacc
     8281 gcactttaga actgtctgcg gcattgcgcc taatgtcgaa ttgatctgga aacgggatga
     8341 ggcgacgcgt tgcgcgtcca ccaagaaagc gcctgaacca acccagtgcc gcaacggatg
     8401 gccgggccgt cgcaacctga tcaaccaagc ggttgctgtt ggcattaaaa aaccggagtt
     8461 gatcacgcat gggtcgattc tgccagtgca aactggcaaa gacatgcaca gaatttactt
     8521 ctgccgttct ttcatcaaat cgcgcagtcg tttgggtgca ggtaccagtg gtgtacggct
     8581 gcgtgtccag gcaccctgct ctgaaggcga caacgcccgg ccccggctca tgaaccagga
     8641 agccatcttg tacaagccca gtttgctgta cacctgtgcc cagacgctcc ataccactga
     8701 cacggccatg ctgcgcccgg caccgcttcc gcgcaaggtg gcatcgtcgc tgtcgggctg
     8761 ggcctgcgct tcgttgcgca aacgcaccag aatatcggtg attggaatct tgaccggaca
     8821 aacctcggca catgccccac acagggtgga ggcaaaagcc agcgggtaag tgacatccag
     8881 gcccagcatg tgcggtgaaa tgatggctcc aattggccct gggtaagtgg tgccgtaggc
     8941 atgaccaccg atacgggcgt agaccgggca atggttcatg caggcaccac atcgaatgca
     9001 ctgcagagtg gcacgcaact cgtcatcagc ataggcctgg gtgcgcccgt tgtccagcag
     9061 gatcagatgc acctcttggg gaccgtcttt ttcgcctggc ttgcgcggcc cacttatcat
     9121 gttcacgtag gttgtcaccg gctggccggt cgccgagcgc gtcagcaggc tgatcaatgg
     9181 cggcaggtgt tcgagcttct ccaccacctt ctcgatgccg gtaatggcaa tgtgaaccct
     9241 cggcacggtg gtgcacatgc gcccgttgcc ctcgttttcg acgagacaaa gcgtgccggt
     9301 ttccgccaca cagaagttca caccagacaa accaatatct gcatcagcaa atttgcgtcg
     9361 cagcacccgt cgaccaatct ggatcaacgc gtccacatct tctgtgtagg ttacgccagg
     9421 cacttcatcg gcaaacagtt gcgcgatttc ctgcttggtt ttgtgaatgg ctggcatgat
     9481 gatgtgcgac ggtgcctcgc cagccaactg cacgatgtac tcgcccatgt cggactcaaa
     9541 ggcttcaatg cctgccgcct ccatggcatg gttgaagccg atttcttcac tgaccatcga
     9601 cttgcccttg atgatgtgcc tggccccgac acgccgggca atacccagcg caatggcatt
     9661 ggcttcttcg ggtgtttggg cccaatgcac ctgaacaccc tgtgccacca gcttggcttc
     9721 gagttgctcc agcaagcgtg gcagatgggc caggctatac cggcgaattt gggcaccaag
     9781 ttccctcagc ccttgcaact cgtcctgatc ggcaaactgg gcacgccgtt tggcctgcaa
     9841 aaagtccatg gccccacgaa aattacggcg ttgccccgca tcattgagca cctgccgact
     9901 gcgctccttg aagtcctgcg taggattgat ggcgatgact ttgctcatgt tgctgctcct
     9961 gctgactggc gcaacagcac taccagctca cgcggcccgt gagccccata ggccagcgtt
    10021 tgctgaatat ctgccgtttt ggacggccca cagatcagca aggcattggt cggcatgcct
    10081 ttggaccagc cttctgcgac catggcagaa taaaagtcgg catggatggt gttcacatcc
    10141 agcaacacaa aatgcaaggg cggcaccagc gacatcaaac ggggttcact cgggctgggc
    10201 cacaaaatca ggctacctgt ctcagctatg gcgctgcggg ccagcgtcac agaggcatca
    10261 atgtcgtcaa acaactggtc ttgccaagca tctaccggct gatcgtagcg cacgattttc
    10321 agtgtacttg gtgcgctcgc ttccagttca gcggcaaccg cgctgtcagc accaaccaat
    10381 aggtggcgca gtcctttgct ggcggcaatt tgcgacaaaa cagccaccca gttggcatcg
    10441 gtcacgtcat gcagctctgt cttcacggcc atcaaggcgg cacgcagccg cgtcacccgc
    10501 tgcgcgctgt cttccgttcg gctgtgcccg gcaaaccagc cagcaacatc cggcagcgca
    10561 ggtatgttgg gcatggtggt ggcgcgcaag cgcgccaata tgtggtcccg cggactcatg
    10621 gcctgactcc ggtgcctggg ttggttcgct gccacaaaaa gctggcaagg tgttgtcccg
    10681 tcaaagacgg ctcttgacaa gcggcaatct catcttgtct ggctgaccgc ccgatgatgt
    10741 tgagcaggca gccacagtcg gcactcacta cgcgctgcac gccgctgtcc ttaatgctgg
    10801 tcaccttgtc ggtgacgatg gcttccgaga tatccgggtg caacagcgaa aacgtgcccc
    10861 caaaaccgca gcattcatca atgcgtgcct gctccaccac cttgacatga cgcagcgtat
    10921 cgagaagtgc agcgctggtt tcgtgggaac ccatctggcg gcgtgcatga caagaagtgt
    10981 gcagcgccac cgtgcattct tccccaaggt cttcgtgact gaaattgacg acatgcacaa
    11041 gaaactcact caactcaaaa acccgttcag acaattcaag cgctttggca tgcagtaccg
    11101 gatcatcggc aaaaagatgt gggtaatgca gccggatcat gcagccgcaa gaaccggacg
    11161 gaaccaccac cggccacggc tgggcaaaca ggttcaactg atgcaatgcc accgcgcggg
    11221 cctcgtcggg gtagccgctg ctatgggccg gttggccgca gcaggtttgt gcttgcgggt
    11281 aatgcacccg gatgccttca cgctcaagca attgaacggt gtcaagccca gcctgcggta
    11341 tgaactgatc taccaggcag gtcgcaaaca aatagatgtc gctgggtttg tccggatact
    11401 ttcgcgcaca aagggtgttg atggccatcg attgtctctt tcgctgttgt cgttgttcag
    11461 ttctgacatt ctcagggcta acagttagcg catgttagag aaatcgaggc ttgggggaga
    11521 aaaaaggcac ttacaaaaat tttacaaaaa atgttgcaat gcaacaaaaa gtgtttctat
    11581 aattcgctca tgctgcactg caacataagc tggattcagc gcaatgacgg ggcagataac
    11641 cttttaactg atggagaact gatcatgatg actgtagagc aaatcctggc cacccaaaaa
    11701 gcaaacattg aaaccctgct gaatttgact gccaaggcct ttgaaggcgt tgagaagatt
    11761 gttgaactca acatgagtgc atccaaggca gctttggcgg aatctggtga aaatgccatg
    11821 gccttactca gcgccaagga tgcgcaagaa ctcttggcac tgcaatcggg actgctgcaa
    11881 ccgttggcgg aaaaaactgc tgcatacagc cgccatctgt acgaaatcgc caccggcacc
    11941 accagcgaat tcaccaagac attcgaaggc caggccgctg aagctcaaca aaaaataatc
    12001 gacctgatcg aatccacagc aaaaaatgca ccagccggat cagaaacagc agttgccgtc
    12061 atgaaaagtg tcgtcgccgc agccagcaac gctctggaat cggtacaaaa agcagtgaaa
    12121 caagcaacag aagtggcaga agccaacttt actgcggcaa gcaacaccgc cacaaccgct
    12181 gtcaaatccg caacaaaaaa gcggtaatct gtatcggata acacacaaat cagaatcaag
    12241 ccaaaattta agcaggcggc tctaaagatc gagggaaaac cctgacatac ttagtcaacg
    12301 tttttgtttt aagccgtctg gcttgcccag gttgtctcct cgggtccttt cgaaaccttc
    12361 aggttcaggg atcccttaag ggttggtcgc aagaccaacc cttttttttg tcaaggatgc
    12421 ccttttgctt ttgcgtccat cagtcggcgt agcgcgggca gttgccgcat catggcttgc
    12481 ctgccgaatt gaatcgcctc aagccgggtc gagaaacttg tactggtaag cccggtcatc
    12541 acaggtttga cgacaacatc tgcccccaga agctcaaagg cgctgatgct tttgcccatg
    12601 atcgcaaatg tctgcatcaa cacctggaaa gtatcttggg catctttgcc atcgggctca
    12661 ctcgagacat cgaccgcaat tatcagatct gcacccattt ttttggcatg actcaccggt
    12721 actggcgaaa ccagaccacc atcaacgtag tctctgcccg cgatggatac tggttgaaat
    12781 accgcaggaa tggcacttga ggcgcgcacc gccgttgcgg tgtcaccacg ctgaaacaaa
    12841 accgccaatc cgctgcgcaa gtcggtggcc accacaccca gaggcatcgg caaatcctga
    12901 atttgcctgc tcttgagatt ggcgctgaca aacctggcca aagcttcgcc ccttaaaaca
    12961 ccaggcttga acaaaggcaa tcgccaatcg gaaaatgatg cttcgtccat cgacaatgcc
    13021 agtttctgca aatcctggcc actcttgccg ctggcgtaca gcgctgccac cagactgccc
    13081 gccgaagtgc ccaccaccag gtcggggtga accccggcct cttctagcac ctgaatcacg
    13141 ccaatatggg caaaacccct ggctgcccca ccccccagca ccaagcccag cctgggtggt
    13201 ttcctgacaa ggggcggcgc tgcttcggat tttgggggca acgttgcctg tggaccgggt
    13261 gcaggcacat tcgcacagcc agcaaccaag gcagccgtga cagcaaacgc gactaatccg
    13321 taagaccagc gagtctgtct cgctttgccc agcacagtga cagcaagcaa gcgatcagct
    13381 cgccgtcact tcctgtgctt ctggccgtga cttgccctct ttcttcggca aaggtgtgat
    13441 ggtgagcaaa acgtctgtct tgctttcatc cttgtcatcg agatccacgg tcaagcggcc
    13501 accatcgatc agccgtccga acaaaagctc gtcggccagc gcgcggcgga tggtgtcctg
    13561 aatcagcctt tgcatcggtc gcgcacccat tagcggatcg aagcctttct tcgccaaatg
    13621 caagcgcaac ttgtcagtga aggtgacttc gactttttta tcggccaatt gactttcgag
    13681 ttgcaacaga aatttgtcga caacgcgcaa aatcacgttc tcatcgagcg acttgaagct
    13741 gacgatggca tcgagccggt tgcggaactc aggcgtaaac aggcgtttga tgtcggccat
    13801 ttcgtcgccc gcctcacgcg ggttggtaaa gccaattgtt gccttgttca tggtttcggc
    13861 acccgcattg gtggtcatga tgatgatcac attgcggaaa tctgccttgc ggccattgtt
    13921 gtcggtcagc gtgccatggt ccattacctg cagcaacacg ttaaaaatcg ccggatgggc
    13981 tttttcgact tcgtcgagca gcaacacaca gtgtggtttc ttggtaatcg cttcggttag
    14041 caaaccaccc tgatcaaacc ccacatagcc aggtggcgca ccaatcaggc ggctcaccgc
    14101 gtgttgttcc atgtactcgc tcatgtcaaa gcggatcagt tcgatgccca tgatgtaggc
    14161 taactgcttg gcagcttcgg tcttgccaac accggtcgga ccgctgaaca aaaaggaccc
    14221 gatgggttta tcacccttgc ccaagccaga gcgagccatc ttgacagaag atgccaacat
    14281 gtcgagtgcc ttgtcctggc cgaacaccac
//
LOCUS       DAOSLL010000032        14286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4341_length_14286_cov_4.924039, whole genome shotgun sequence.
ACCESSION   DAOSLL010000032 DAOSLL010000000
VERSION     DAOSLL010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 14286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14286
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4341_length_14286_cov_4.924039"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..920
                     /locus_tag="PLB25_02410"
                     /pseudo
     CDS             1..920
                     /locus_tag="PLB25_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794022.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
     gene            898..1503
                     /gene="ampD"
                     /locus_tag="PLB25_02415"
     CDS             898..1503
                     /gene="ampD"
                     /locus_tag="PLB25_02415"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HOE40478.1"
                     /translation="MSATKPFEHAPDNSCWHDGWHTLANRLPSENHGARPPLASIDLI
                     VLHSISLPPGQYGGDQVQQLFTNRLDWQAHPYFQRIAGITVSAHFYIRRDGELWQFVS
                     CNDRAWHAGLSSYRGRSNCNDDSIGVELEGLEGDVFESAQYVALVTLCAAIMQRYPIL
                     HIAGHEHVAPGRKFDPGPGFDWLLLKKALGLGDQYLPLVTV"
     gene            1594..1770
                     /locus_tag="PLB25_02420"
     CDS             1594..1770
                     /locus_tag="PLB25_02420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40479.1"
                     /translation="MPSMSVLVRLPILVWVALDQFSAEAGSLQGDGQTIADADADADA
                     DADANAVAVTCSWN"
     gene            1842..2642
                     /gene="hisF"
                     /locus_tag="PLB25_02425"
     CDS             1842..2642
                     /gene="hisF"
                     /locus_tag="PLB25_02425"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HOE40480.1"
                     /translation="MLAKRIIPCLDVTGGRVVKGINFVELRDAGDPVEIAARYNEQGA
                     DELTFLDITATSDDRDLILHIIEAVASQVFIPLTVGGGVRTVDDVRRLLNAGADKTSF
                     NSAAIANPHVIEDASRKYGAQCIVVAIDAKRRTAADALRLDVNGRTMGAGWDVYSHGG
                     RKNTGLDALTWARQMAEFGAGEILLTSMDRDGTKAGFDLALTRAVSDAVGVPVIASGG
                     VGTLDHLADGIQQGGADAVLAASIFHYGEFTVAQAKARMAERGIPVRV"
     gene            2765..3163
                     /gene="hisI"
                     /locus_tag="PLB25_02430"
     CDS             2765..3163
                     /gene="hisI"
                     /locus_tag="PLB25_02430"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HOE40481.1"
                     /translation="MNFLDAIKWDAQGLVPVIAQEQGTGDVLMFAWMNRQALQKTAEL
                     GRAVYFSRSRNRLWFKGEESGHVQTVHEIRIDCDADVVLLKVTQIGHTPGIACHTGRH
                     SCFFSIYENGTWKAVDPVLKDPQTIYQRAL"
     gene            3160..3552
                     /locus_tag="PLB25_02435"
     CDS             3160..3552
                     /locus_tag="PLB25_02435"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HOE40482.1"
                     /translation="MRPNPSSMDSLAALAAVIESRLPARGGDPQASYVARLLHKGPDA
                     FLKKIGEEACEVVMAAKDADHGGDKAKIVYEVADLWFHTMVALAHYGLSPADVLAELE
                     RRAGTSGIEEKAMRKVQAREATLNGALP"
     gene            3549..3968
                     /locus_tag="PLB25_02440"
     CDS             3549..3968
                     /locus_tag="PLB25_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40483.1"
                     /translation="MTQDFSQHDPSPGSVDGVDLQQINALNTIGTISYVLHLIVAVSA
                     LIPGGQFGPILLIAALLIDLVKRDDARGTWHASHFRWRIRTVIIAGVLYLVTFPLYLL
                     LYFPGAIAWLVISIWFLYRIVTGMVRMNKGRPMEFST"
     gene            3965..4339
                     /locus_tag="PLB25_02445"
     CDS             3965..4339
                     /locus_tag="PLB25_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HOE40484.1"
                     /translation="MIELVPQTDPDCLFCKIVAGKIPSKLVYQDDEFYAFHDIHPSAP
                     VHFMIIPKSHIASMALLTDDHVAMMGRMMVLVPKLALQAGCRPYPAGGFRVVCNTGAD
                     GAQDIHHLHIHVMGGPRPWLRG"
     gene            4408..4653
                     /gene="tatA"
                     /locus_tag="PLB25_02450"
     CDS             4408..4653
                     /gene="tatA"
                     /locus_tag="PLB25_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HOE40485.1"
                     /translation="MGSFSIWHWLIVLVIVVIVFGTKKLKNIGSDLGGAVKGFKDGMR
                     DGGTAPAEDKPAAPAQVVSAVPAADKTTIDVEAKQKS"
     gene            4684..5151
                     /gene="tatB"
                     /locus_tag="PLB25_02455"
     CDS             4684..5151
                     /gene="tatB"
                     /locus_tag="PLB25_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003062149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HOE40486.1"
                     /translation="MIDLGISKLALIGAVALIVIGPEKLPRVARTVGTLLGKAQRYVA
                     DVKAEVSRSMELDELKKMKETVESAARDVNQSMQSGVADLEKSWAQATDIATESSSGL
                     DVPDSVPEYRHPKKNWRLKQTAVPNWYKARAGVRMHAQSGAARVARFRPTRFH"
     gene            5151..5942
                     /gene="tatC"
                     /locus_tag="PLB25_02460"
     CDS             5151..5942
                     /gene="tatC"
                     /locus_tag="PLB25_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HOE40487.1"
                     /translation="MADSPKKDDELAGTEQPFVQHLVELRDRLVKASIAIVVVAIALA
                     AYPGPSALYDLMAAPLVAHLPAGSTLIATSVISPFMVPLKVLLMAAFLIALPLVLYQL
                     WAFVAPGLYSHEKKLVLPLVISSTLLFFIGIAFCYFFVFGRVFAFIQSFAPKSITASP
                     DIEAYLSFVLSMFLAFGMAFEVPVVVVVLARMGIFSIEKLKEFRGYFVVLAFIIAAIV
                     TPPDVVSQLALAIPMCLLYEVGIWAAQIFIRHTKAPDEAATTSSR"
     gene            complement(5947..7089)
                     /locus_tag="PLB25_02465"
     CDS             complement(5947..7089)
                     /locus_tag="PLB25_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HOE40488.1"
                     /translation="MKRFWLLFSQSVTVLLAAYFVVATLKPGWLNQRTGSAGAVAVIE
                     SPATVATAAPPGSFRLAAQKSSSAVVSINTSKTARQGPHSNDPWFKFFFGEQNNQPQV
                     GLGSGVIVSDAGYILTNNHVVENADEIEVILNDSRRAPAKVIGTDPDSDLAVLKIELD
                     RLPVIVLGNSNALQVGDQVLAIGNPFGVGQTVTSGIVSALGRNQLGINTFENFIQTDA
                     AINPGNSGGALVDTNGNLLGINTAIYSRSGGSMGIGFAIPVSTAKMVLEGIIKDGQVT
                     RGWIGVEPNELTQELAQTFGVATRQGVIITGVLQNGPAASGGMKPGDVVTRVANQDIH
                     NVPDLLTAVSALKPGTAVVFEVLRTSGKVALQIAPGVRPKPRQPAQ"
     gene            7223..7585
                     /locus_tag="PLB25_02470"
     CDS             7223..7585
                     /locus_tag="PLB25_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40489.1"
                     /translation="MPSTQTIVHIFAQAPAKPVLGAPCNGCGVCCLLEPCPLGVVLSG
                     RRTGACLALRWQPDLRLYRCGAITRPSDVLQDRLPRCLQFLSPALAWALVRWARRWVA
                     AGLGCDCDSEVMPACPKT"
     gene            7704..8462
                     /locus_tag="PLB25_02475"
     CDS             7704..8462
                     /locus_tag="PLB25_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HOE40490.1"
                     /translation="MTTRGELLRAFDGLLQPERFKDYAPNGLQVEGVDRVRKMVSGVT
                     ASRALIEAAIAAQADTIFVHHGLFWRGQDGRVTGWMKQRLALLLAHDINLFAYHLPLD
                     AHPELGNNAQFGLKIGLVSRSRFGEQDLCFMGERADGQAFASAQALAEHLKSVLNRPV
                     ALVDTAQTAIKKIAWCSGGAQSYFEAAIAAGADAFITGEISEPQAHLARECGVVYLAC
                     GHHASERYGAPAVAAHVATQFGLQHAFIDIDNPA"
     gene            8485..9552
                     /gene="pdxA"
                     /locus_tag="PLB25_02480"
     CDS             8485..9552
                     /gene="pdxA"
                     /locus_tag="PLB25_02480"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HOE40491.1"
                     /translation="MTTHTTLPIAITQGDPAGIGPEIIVKAYRDAPAQTLGCFVAGDL
                     ATLRRAAQIVAGDSVAMPVALLSNAQEALNCPPRCIPLLQIGSSSAAEAQDPVPFGRL
                     AARAGRMAGDCVVWAANAALQGEVAALVTAPLNKAALSLAGAPYSAFPGHTEMLQALA
                     AAHTQQTTAQLPVRMMLANDELRVVLVSIHLSLRDALAAVTFDNVLQTLLITHQSLSA
                     MLGRAPRIGVAGLNPHAGEGGLFGSEERDIIGPAIAAARRQGLQVSEPLAPDTVFMRA
                     RAKGGQPAEFDVVLAMYHDQGLIPVKYLGVEKGVNVTLGLPLVRTSPDHGTAFEIAGT
                     GQADASSLIEAIRVARQLAKS"
     gene            complement(9610..10038)
                     /gene="mscL"
                     /locus_tag="PLB25_02485"
     CDS             complement(9610..10038)
                     /gene="mscL"
                     /locus_tag="PLB25_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HOE40492.1"
                     /translation="MGMMTEFKEFAVKGNVMDLAVGVIIGGAFGKIVESVVGDMVMPI
                     VGAIIGKLDFSNLFIALGQIPEGTARTLADLKKAGVPVFAYGNFITVAVNFAILAFII
                     FMMVKQINRLKKQAPAEPEAAPATPEDVVLLREIRDSLKK"
     gene            10207..10821
                     /gene="petA"
                     /locus_tag="PLB25_02490"
     CDS             10207..10821
                     /gene="petA"
                     /locus_tag="PLB25_02490"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HOE40493.1"
                     /translation="MSEAFAESNLNDASKRTWLIASGCAGAVGGVAAVVPFVSSFLPS
                     EKAKAAGAAVEADISALVPGEKMTVEWRGKPVWIVRRTPEQLAALPKLNDQLADPKSE
                     RKPAEQAPEYARNEVRSRKPEYLVVVGICTHLGCSPSDKFKPGPQPSLPDDWQGGFFC
                     PCHGSTFDLAGRVYKNKPAPDNLEVPPHMYLSDTKLLIGEDKKA"
     gene            10833..12233
                     /locus_tag="PLB25_02495"
     CDS             10833..12233
                     /locus_tag="PLB25_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HOE40494.1"
                     /translation="MAEFHEISPNAPMGQKALNWVDNRFPLSKLFKEHMSEYYAPKNF
                     NFWYIFGSLSLLVLVIQIVTGIFLVMHYKPDAAVAFASVEYIMRDVPWGWLIRYMHST
                     GASAFFVVVYLHMFRGLMYGSYRKPRELIWLFGCGIFLALMAEAFMGYLLPWGQMSYW
                     GAQVIVNLFSAIPFIGPDLALLIRGDFVVGDATLNRFFSFHVIAVPLVLLGLVVAHII
                     ALHEVGSNNPDGVEIKASKDAKGIPLDGIPFHPYYSVHDVFGVSVFLFVFSAVVFFAP
                     EMGGYFLEYNNFIPADPFQTPLHIAPVWYFTPFYTLLRAVTTEMMYVLIACVIIGAVL
                     AVAKIKMPALFKGLIVGGAVVIAALMMAIDAKFWGVVAMGGGVVILFFLPWLDNSPVK
                     SIRYRPDWHKYLYAVFVINFFVIGYLGTQPVSVIAGRISQAGTLFYFGFFILMPWWSR
                     LGTFKTVPSRVTFTAH"
     gene            12249..12938
                     /locus_tag="PLB25_02500"
     CDS             12249..12938
                     /locus_tag="PLB25_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HOE40495.1"
                     /translation="MKTMGFTQKIVLALILALGLTASVQANVAGPAWDKAPDKIKDMA
                     ALQSGAKTFVNYCLNCHSAAFMRFNRLQDIGLTSEQIRDNLLVTNGKLGETMKAAIDP
                     QQAKAWFGVNPPDLTVIARSRAGAGGTGTDYLYSYMRGFYKDETKPTGWNNHVFPNVA
                     MPHVLWEMQSTMTPAQYDQTMGDLVNYLQWMAEPAQTARKTIGMWVLVFLAGLTFLTW
                     RLNAAYWKDIK"
     gene            13038..13649
                     /locus_tag="PLB25_02505"
     CDS             13038..13649
                     /locus_tag="PLB25_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006999756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HOE40496.1"
                     /translation="MMVLYSGTTCPFSHRCRFVLFEKGMDFEIRDVDLYNKPEDINVM
                     NPYGQVPILVERDLILYESNIINEYIDERFPHPQLMPGDPVDRARVRLFLLNFEKELF
                     AHVSTLESRTAKSNEKAVEKARAHIRDRLTQLAPVFLKNKFMLGDGFSMLDVAIAPLL
                     WRLDYYGIELSKNAAPLLKYAERIFSRPAYIEALTPSEKVMRK"
     gene            13662..14189
                     /locus_tag="PLB25_02510"
     CDS             13662..14189
                     /locus_tag="PLB25_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HOE40497.1"
                     /translation="MSVNQKPEATSTRPYLIRALHEWCCDNGLTPYITVAVDNSVQVP
                     REYVKDDEIVLNIGFDATTALSMGNEYLEFKARFSGAVRDIMVPMDRISAIFARENGQ
                     GMAFPMVDRSEQNRSSRKETEPRPAVSLALAQKKLGLNGDIEGGDLDNDSDTRPSPQS
                     QGSNPGAKPSLTRVK"
BASE COUNT         2965 a         3857 c         4110 g         3354 t
ORIGIN      
        1 gcgagtcagg tactggtaaa gaactggtcg ccgatgcgat ccataccacc agccatcgcg
       61 cctaaggacc atgtgttgcc gtgaactgtg gcgccattcc cgagaatctt ctggaggcgg
      121 agttctttgg tgtaaaaaaa ggtgcctaca ccggtgccca gcaggatcgc gaaggctttt
      181 ttcaggccgc caggggtggc accttgtttc ttgacgagat cggtgacttg ccattggcca
      241 tgcagtccaa gttgttgcgt gcgattcagg agcggcgcat tcgaccgctg ggttcgacgc
      301 aagaggaact ggtcgatgtg cggattgtga gcgcgaccca taagaatctg gcacaagagg
      361 ttcaggatgg tcaattccgg caagacctgt tttaccgttt gaacgtcatt gacatggtga
      421 ttgcaccctt gcgggaacga agcgaggact tgcccgagtt gtgcgctgcc ttgttggcgc
      481 ggatcgcaga tgaatctggt attgccgtgc ccatccttgg ggatgcagtg cttgaggaac
      541 tctcgcacct gccaatgccc ggcaatgtgc gcgaactgga gaatctgctg taccgggcgg
      601 ttgccctggg cgacggccgt agcgtgcaaa tcgaagttcg atcacaactc attccaatgg
      661 cggcaaacag tttggtgaac tccttgccgg tcggcccgca agaccccgtc accccaattc
      721 cagccgatct gcaaagccat ctggacaaac ttgagcgtga gattttgcaa caagcactga
      781 aggaaacagg ctttaatcgc acagccgccg ccgccagact gggattgagt ctgcgtcaga
      841 tgcgctaccg tatcacccga ttgcagattg aatcccccca aaacggcgaa gccagcgatg
      901 agtgcgacga aaccatttga acacgcgcca gacaactcct gctggcatga cggctggcac
      961 acattggcca atcggttgcc ttctgagaac cacggagcgc gtccgccgct ggccagcatt
     1021 gatttgattg ttcttcactc gatcagcctg ccgccgggtc agtacggtgg tgaccaggtt
     1081 cagcagcttt tcaccaaccg gcttgactgg caggcacatc cctacttcca acgcattgca
     1141 ggcatcacgg tatcggcgca tttttatatt cgccgcgacg gtgaactttg gcaatttgtc
     1201 agttgcaacg accgggcctg gcatgccggc ttatcaagtt accggggccg cagcaactgc
     1261 aacgacgaca gcattggtgt tgaacttgaa ggtctggagg gcgatgtttt cgaaagcgcg
     1321 caatacgttg cgctagtcac cctttgcgct gccatcatgc agcgctaccc aattttgcat
     1381 atcgctggtc acgaacatgt ggcaccgggc cgaaaatttg atccaggacc aggctttgac
     1441 tggctgctat tgaaaaaggc gctgggcctg ggcgatcagt atttaccctt ggtcacggtt
     1501 tagcaatcgc gctgaacctc cctttatgcg ttccaaaaat cctgaaagca actacaggca
     1561 tgctttgccc ccatcaaaat gacaaggact gccatgccgt caatgtcagt gctggttcga
     1621 ttgccgatac tggtctgggt tgcccttgat cagttcagtg ccgaggcagg aagtttgcag
     1681 ggcgatggtc agaccatcgc cgatgccgat gccgatgccg atgccgatgc cgatgccaac
     1741 gcagtagctg ttacctgttc atggaactga attggcggca gcagctttgc accttaagcc
     1801 cttcacaatg tcgatttact cattgagcag tcaaaaaaac catgttagcc aaacgaatca
     1861 ttccctgtct tgacgtgacc ggtggccgcg tcgtcaaagg catcaatttt gtcgaattgc
     1921 gcgatgccgg tgacccggtg gagatcgccg cgcgttacaa cgagcagggg gccgacgaac
     1981 tgacctttct cgacatcacc gccaccagcg acgaccgtga cctgatcttg cacatcatcg
     2041 aagcggtggc cagccaggtg tttattccgc tgacggtcgg cggtggcgtg cgcacggtcg
     2101 atgacgtgcg tcgcctgctc aacgccgggg ccgacaagac cagcttcaat tcggcggcga
     2161 ttgccaaccc tcatgtcatt gaggatgcct cgcgcaaata cggtgcccaa tgcatcgtgg
     2221 tggcgattga tgccaagcgc cgtactgctg ccgatgcgtt acggctggat gtcaacgggc
     2281 ggacgatggg cgcaggctgg gacgtgtaca gccacggtgg gcgcaagaac accgggctcg
     2341 atgcactgac ctgggccagg caaatggctg agtttggcgc gggagagatt ttgctcacca
     2401 gcatggaccg cgatggcacc aaggccggat ttgatctggc gctgacccgc gcggtgagcg
     2461 atgcggtcgg cgtgccggtg atcgcctcag gcggcgttgg cacgcttgat caccttgccg
     2521 atggcattca gcagggcggt gccgatgcgg tgttggccgc gagcattttt cactacggcg
     2581 aattcaccgt ggcgcaagcc aaggcgcgca tggccgagcg cggcataccg gtgcgtgttt
     2641 gagccaatca cagccgcacc cagtccggtt aggtacttat caaagaaaat ggcctccagc
     2701 ccttggtatc attgcggttg aagctatata aatagtagtg tttcaaatca tcgacaattt
     2761 cgccatgaat tttctcgatg cgattaaatg ggacgcgcaa ggcttggtgc cggtgattgc
     2821 acaagagcag ggcacgggcg acgtgctgat gtttgcctgg atgaaccgtc aggcgctgca
     2881 aaagactgcg gaactgggcc gtgcggtgta tttcagccgc tcccgcaaca gactctggtt
     2941 caagggcgag gagtccggcc atgtgcagac ggtgcatgaa atccgcatcg actgcgatgc
     3001 cgatgtggtg ttgctcaaag tcacgcaaat tggccacaca ccgggtattg cctgccacac
     3061 cggtcggcac agttgttttt tcagtatcta tgagaatggc acatggaaag ctgtcgatcc
     3121 ggtactgaaa gacccacaaa ccatttacca aagagcgtta tgagacccaa cccatcgtcc
     3181 atggattcac tggccgcact tgctgccgtg atcgaaagcc gcctgccagc ccgtggcggc
     3241 gacccgcaag ccagttatgt cgcgcgcctg ttgcacaagg ggccagatgc gttcctgaaa
     3301 aagattggcg aggaagcctg cgaggtggtg atggccgcca aggatgccga ccacggtggc
     3361 gacaaggcca aaattgtcta tgaagtggcc gacctgtggt ttcacaccat ggtggcgctg
     3421 gcccattacg gcttgagccc ggctgacgtg ctggccgaac tggagcgccg cgccggcacc
     3481 agcggcatcg aggaaaaagc catgcgcaag gtgcaggcac gtgaagcaac actcaacgga
     3541 gcattgccat gacccaagat ttcagtcagc atgacccttc accgggctcg gtcgatggtg
     3601 ttgacctgca acagatcaat gcgctcaaca ccattggcac catcagctat gtgctgcacc
     3661 tgatcgtggc ggtcagtgcg ctgataccgg gcggccaatt cgggccgatc ttgctgatcg
     3721 cggcgctgct gatcgatctg gtcaaacgcg atgacgcacg cggcacttgg cacgcgtcgc
     3781 acttcaggtg gcgcattcgc accgtcatca ttgcgggtgt gttgtacctg gtcacgtttc
     3841 cgctgtattt gctgctttac tttccaggcg caatcgcctg gctggtcatt tcaatctggt
     3901 ttttgtaccg catcgtgacc ggcatggtgc gcatgaacaa gggccgaccc atggagtttt
     3961 caacatgatc gaactagtgc ctcagaccga tccagattgc ctgttctgca aaatcgtagc
     4021 aggcaagatt ccttccaaat tggtctatca ggacgatgag ttttacgcct ttcatgacat
     4081 ccacccttcg gcaccggtgc atttcatgat cattccgaaa tcgcatattg cgtcgatggc
     4141 gctgctgact gacgatcacg tggccatgat ggggcgcatg atggtgctgg tgcccaaact
     4201 ggcgctgcaa gcgggttgcc gaccctatcc ggcaggcggc ttccgggtgg tctgcaacac
     4261 tggcgctgac ggcgcacagg atattcacca cctgcacatt cacgtcatgg gtggcccccg
     4321 accctggttg cggggctaaa ttgggttcat gcggcgttgt gctggagcct gccgcttaaa
     4381 attcgcgcaa cttctcagga gaccgcaatg ggttcatttt cgatttggca ttggttgatt
     4441 gttttggtga tcgtggtgat cgtatttggc accaagaagc ttaaaaacat cggcagtgac
     4501 cttggcgggg ccgttaaggg cttcaaggac ggcatgagag atggcggcac agcacctgct
     4561 gaggacaagc ctgccgcacc cgctcaggtg gtcagcgccg tgcctgcggc tgacaaaacc
     4621 acgattgacg ttgaagccaa gcaaaagtcc tgatgaatca gtctgctgtc gcgcaaggct
     4681 tgcatgattg acctgggtat ctcgaaactg gcgttgattg gtgcggtggc gctgatcgtg
     4741 attggccccg aaaagctgcc acgcgtggcg cgcaccgtcg gcaccttgct gggcaaggcg
     4801 cagcgctatg tggccgatgt gaaggccgag gtcagccgct cgatggaact tgatgaactc
     4861 aagaagatga aggaaacggt ggaaagcgcg gccagggatg tgaaccagtc gatgcagtct
     4921 ggtgttgccg atcttgagaa atcctgggca caagccactg acatcgcgac cgaaagcagt
     4981 tccgggcttg acgtgcctga ctcggtgcct gaataccggc accccaaaaa gaactggcgg
     5041 ctcaagcaga cggcggtgcc caattggtat aaggcccgtg ccggtgtgcg catgcacgcc
     5101 cagtcggggg ccgcgcgggt cgcgcgtttt cgcccaacca ggtttcattg atggcagatt
     5161 cccccaaaaa agacgacgaa ctggccggca ccgagcagcc cttcgtgcag cacctcgtgg
     5221 agctgcgtga tcgcttggtc aaggcaagca ttgccatcgt ggtggtggcg attgccctgg
     5281 cggcctatcc ggggccgtcc gcgctgtatg acctgatggc ggcacccttg gtggcccact
     5341 tgccagccgg ctcaacgctg attgcgacct cggtcatttc accttttatg gtgcctctca
     5401 aggtgctgtt gatggcggct ttcctgattg cactgcccct tgtgctgtac cagttgtggg
     5461 cgtttgtcgc accgggcttg tattcgcacg aaaagaaact ggttttgccg ctggtgattt
     5521 ccagtacctt actgttcttt atcggtattg cgttttgcta cttttttgtg tttggccggg
     5581 tttttgcctt tatccagagt ttcgcgccca agagcattac cgcgtccccc gacattgaag
     5641 cctatttgag ttttgtgctg tcaatgtttc tggcttttgg catggcgttt gaagtgccgg
     5701 tggtggtggt ggtgctggcg cgcatgggca ttttcagcat cgaaaagttg aaagagtttc
     5761 gcggttattt tgtcgtgctg gcgttcatca ttgcggccat cgtgacgccg cccgatgtgg
     5821 tgtcgcaact ggcattggcc attcccatgt gcctgctgta tgaagtcggc atctgggcgg
     5881 ctcagatttt catccggcat accaaggcac ccgacgaagc cgcgacaacc tcgtcgcgct
     5941 gatcgcttat tgggcgggct ggcgtggttt agggcgaacg cccggcgcaa tttgcaaggc
     6001 aactttgcca gaagtgcgca gcacctcaaa cacaaccgca gttccgggct tcaaagccga
     6061 aacagcggtc agtaaatcag gcacgttgtg gatatcctgg ttggccacgc gggtaacaac
     6121 atcaccgggt ttcatgcctc ctgaggcggc tggcccgttt tgcagcacac cagtgatgat
     6181 gaccccctgc cgggtcgcca cgccaaatgt ctgggccagt tcctgagtca gttcattggg
     6241 ctccacgcca atccagccgc gcgtgacctg tccgtccttg atgatgcctt ccagcaccat
     6301 tttggcggtt gataccggaa tggcgaaacc aatccccatg ctgccaccag agcgcgaata
     6361 aattgccgtg ttgatgccca gcaggttgcc gttggtatcg accagcgcac cgcccgagtt
     6421 gccggggttg atggcggcat cggtctgaat gaagttttcg aaggtgttga tgcccaactg
     6481 gttgcgcccg agcgcgctga cgatgccgct ggtgacggtt tggccgacac caaacgggtt
     6541 gccgatggcc agcacttggt cacccacctg tagtgcattg gagttgccca gcacaatcac
     6601 cggtagtcgg tccagttcaa ttttcagtac cgccaggtcc gaatcggggt cggtgccaat
     6661 gactttggcc ggtgcacgtc ggctgtcgtt gaggatgacc tcaatttcat cagcgttttc
     6721 gaccacgtgg ttattggtca ggatatagcc tgcgtcgctg actatcacgc cgctgcccaa
     6781 acccacttgg ggctgattat tttgctcgcc aaaaaagaac ttgaaccatg ggtcattact
     6841 gtgtggaccc tgtcgggcag tcttgctggt gttgatgctg acgacggcag acgaggactt
     6901 ttgcgccgcc agccgaaaac tgccgggcgg cgctgcggtg gcaaccgttg ccggcgactc
     6961 aatgacggcc actgcaccgg ctgaacccgt gcgctgattg agccagccag gtttaagcgt
     7021 ggccaccaca aagtaggcgg ccagcagcac cgtgaccgac tgggaaaaca ataaccaaaa
     7081 acgtttcatg gacaatagcg gaggcaagtc cggcgttggg aatgggcttg attgcagtca
     7141 gtttgctagg ttgctggcgc gacctggcca tcaatccggg agcaaactgt gtggataaat
     7201 tgtaggtgca ccgcttattt caatgcccag tacgcaaacc atcgtccata tttttgccca
     7261 ggcaccagcc aaacctgtgc tgggtgcacc ctgcaatggc tgcggtgtct gttgtctgct
     7321 ggaaccctgt ccgctggggg ttgtgctctc cgggcgtcga accggtgcct gcctggccct
     7381 gcgctggcag ccagacctgc ggctataccg ttgcggtgcc atcacccggc cgagtgatgt
     7441 gctgcaggac cgattgccgc gctgcctgca gtttttgtca cccgcgctgg cgtgggcttt
     7501 ggtgcgttgg gccagacgct gggtcgctgc cgggcttggc tgcgattgcg attctgaagt
     7561 gatgccagct tgcccgaaga cttgattcgg gcatggtttg tcagcttcga ttggcttggg
     7621 tacaagggtg atgccttgca tttggtcaat ggccgcttgc aagcgcactt atcatggctc
     7681 actttgctgg aaagtggtgg cctatgacaa cccgtggtga actgttgcgt gcttttgatg
     7741 gcttgttgca gcccgagcgc tttaaagact acgcacccaa cggcctgcag gtcgaaggtg
     7801 ttgaccgggt gcgaaagatg gtctctggtg tcacggccag ccgggccctg attgaggccg
     7861 ctattgcggc gcaggctgat accatttttg tccaccatgg tttgttctgg cgcggtcagg
     7921 acggtcgtgt cactggctgg atgaaacagc gcctagcctt gctgctggcg catgacatca
     7981 acctgtttgc ttatcacttg ccgctggatg cgcaccctga actcggcaat aacgcacaat
     8041 ttggcttgaa aatcgggttg gtgagccgct cgcgctttgg cgagcaagac ctgtgcttca
     8101 tgggtgaacg ggcagacggt caggcgttcg ctagcgccca agcgctggca gagcacttga
     8161 aatcagtact aaatcggcct gtagcccttg tagatactgc gcagacagct ataaaaaaaa
     8221 tagcttggtg cagtggcgga gcgcaaagct attttgaggc agcgatagcc gccggggcgg
     8281 atgccttcat caccggtgag atttcggagc cgcaggccca cctggcacgc gaatgcggtg
     8341 tggtctatct cgcctgcggt caccatgctt ccgagcgtta tggcgcgccc gcagtggccg
     8401 cgcatgtggc gacacaattc ggcctgcagc atgcattcat cgacattgac aacccggctt
     8461 gattgccaaa cgagagcaac tcacatgacc actcacacca cattgccaat tgccatcacc
     8521 cagggcgacc ccgctggtat cggccccgag atcatcgtca aggcctaccg ggatgccccg
     8581 gcgcagaccc tgggttgctt tgtggcaggc gacctggcca ccctgcgccg cgcagcccag
     8641 atagtggcgg gtgattctgt ggccatgccg gtggccctgc tgagcaacgc acaagaggcc
     8701 ttgaactgcc cgccgcgttg cattcccttg ctgcaaattg ggtcgtcaag cgccgccgaa
     8761 gcacaagacc cagtaccttt cggtcgcctg gcagcgcggg ctggccgaat ggcaggcgac
     8821 tgcgtggtgt gggccgccaa tgctgcgctg caaggtgaag tggcggcgtt ggtgacagcg
     8881 ccgttgaaca aggcggccct gtcattggct ggcgcaccgt acagcgcgtt tcctggtcac
     8941 actgaaatgt tgcaggcgct ggcagcagcc catacacagc aaaccacagc gcagttaccg
     9001 gtgcgaatga tgttggccaa tgacgaactg cgggtggtgc tggtcagtat tcacctgtcc
     9061 ttgcgcgatg cgctcgcggc cgtcactttt gacaatgtgc tgcaaaccct gttgattacc
     9121 caccaatcac tttctgccat gctgggacgc gcgccacgta ttggggtcgc aggactcaat
     9181 ccgcatgcgg gcgaaggtgg tttgtttggc tctgaagaac gggacatcat tggtcctgcc
     9241 atcgcggcag cacgtcggca aggtctgcag gtcagcgaac cgctggcccc tgacacggtg
     9301 ttcatgcggg ctcgcgcaaa gggcgggcag ccagccgaat ttgacgtggt gctggcgatg
     9361 taccacgacc agggcttgat cccggtaaag tacctgggcg tggaaaaagg ggtgaatgtg
     9421 acactcggcc tgccactggt tcgcaccagt cccgaccatg ggacagcgtt cgaaattgcc
     9481 ggcacaggtc aagcggatgc ttcaagtttg atcgaagcca tccgggtcgc gcgacagttg
     9541 gcaaaaagtt gactaaatcg gcctgcagcc cctgtcaata aagcacatac agctacaaat
     9601 aaggtagcac tatttcttca ggctatcgcg tatttcacgc agcagcacga cgtcttcagg
     9661 cgttgcaggg gctgcttctg gctcggcagg cgcttgtttt ttcaaccggt tgatttgttt
     9721 gaccatcatg aaaatgatga aagccaaaat cgcaaaattg actgccacag tgatgaagtt
     9781 gccataggca aaaaccggca cgccagcctt tttcaaatcg gccagcgtcc ttgccgtccc
     9841 ttctggaatc tgcccgagag cgatgaacag attggaaaaa tcaagcttgc ctatgatggc
     9901 cccgacgata ggcatcacca tatcgccgac cacagactca acaattttgc cgaaagcgcc
     9961 accaatgatc acgcccacgg caaggtccat cacattgccc ttgactgcaa actctttgaa
    10021 ttcagtcatc attcccatag tttttccttt ctgagttggt taaaacgcgc agagtatcgc
    10081 gtaaatgggt cacggacctg gcaaaacctg cttgccaaac ctgaaaagtg gtcagcttcg
    10141 cgacaaagtg gctcagttac aatcaaaagc tgttatctat tgtggtcttt atcattgagg
    10201 attcccatga gtgaagcttt tgccgaaagc aacctaaatg acgccagcaa acggacatgg
    10261 ctgattgcgt ccggttgcgc tggtgccgtt ggcggtgtcg cagcagtcgt cccttttgtc
    10321 agctcttttt tgccgtccga aaaggccaag gccgcaggag cggctgtcga agctgatatt
    10381 tcggctctcg tgcctggcga aaaaatgacg gtggagtggc gcggcaagcc agtttggatt
    10441 gtccggcgaa cgcctgagca acttgccgca ttgccaaaac tcaacgacca gttggcagat
    10501 ccaaagtccg agcgcaagcc ggccgagcag gctcctgagt atgcccgcaa tgaggtccgc
    10561 tcccgcaagc ctgagtatct ggtagttgtc ggtatttgta cccatctcgg gtgttctcct
    10621 tcggacaaat tcaagcctgg tccgcaaccg tcgttgcctg acgattggca aggtgggttt
    10681 ttctgcccct gtcacggctc cacctttgat ctggccgggc gtgtctataa aaacaagcca
    10741 gcaccggata accttgaagt gccgcctcat atgtacctct cagacaccaa attgctcatt
    10801 ggtgaagaca agaaagccta aggacgaacg acatggctga atttcacgaa atttccccga
    10861 atgccccgat gggccaaaag gcactcaact gggttgacaa ccggtttccg ttgtccaagc
    10921 tcttcaaaga gcacatgagc gagtactacg cgccaaagaa cttcaatttc tggtacatct
    10981 ttggctcttt gtccctgctg gtgctggtga tccagatcgt taccggcatc ttcttggtga
    11041 tgcattacaa gccagacgct gctgtggctt ttgcatcagt cgagtacatc atgcgtgacg
    11101 tgccctgggg ctggctgatc cgctacatgc actccaccgg tgcctcggct ttttttgtgg
    11161 tcgtgtacct gcacatgttt cgggggttga tgtatggcag ctaccgaaaa ccacgtgagc
    11221 tgatctggct ttttggttgt ggcatctttt tggccctgat ggccgaagcc ttcatgggct
    11281 acctgttgcc ttggggccag atgagttact ggggcgcgca ggtgatcgtg aatttgtttt
    11341 ctgccattcc cttcattgga ccagatctcg ccttgctgat ccgtggcgac tttgtggtgg
    11401 gtgatgccac actgaaccgc ttctttagct tccacgtgat tgcggtgccg ttggtcttgt
    11461 tgggtttggt ggttgcgcac atcattgctt tgcatgaggt cggctccaat aaccctgacg
    11521 gtgttgaaat caaggcaagc aaagatgcca aaggcattcc gctcgacggc attccattcc
    11581 atccgtacta ctctgtacat gatgtgtttg gtgtcagtgt cttcctgttt gtcttcagcg
    11641 cggtagtgtt ttttgcgcct gagatgggtg gctatttcct tgaatacaac aatttcattc
    11701 cggccgatcc attccagacc ccgttgcaca tcgcaccggt gtggtacttc acaccgttct
    11761 acaccctgct gcgtgccgtg acgaccgaaa tgatgtacgt gctgattgcc tgtgtgatca
    11821 tcggcgcggt gctggcagta gccaagatca agatgcccgc gcttttcaag ggcttgatcg
    11881 tgggtggtgc agtcgtgatt gccgcattga tgatggccat tgatgccaaa ttctggggtg
    11941 tggtggcgat gggcggtggt gttgttattc tgtttttcct gccgtggttg gacaacagcc
    12001 cggtcaagtc tattcgctac cgtccggatt ggcataaata cctatacgcc gtttttgtca
    12061 tcaacttctt tgtcatcggc tatctgggaa cacaaccggt gtcggtcatt gccggccgta
    12121 tttctcaggc aggcaccttg ttctacttcg gcttttttat cctgatgccc tggtggagtc
    12181 gtcttggtac ctttaaaaca gtgcccagtc gcgttacctt cacggcgcat tgagcaggag
    12241 cataaaacat gaaaaccatg ggtttcacgc aaaaaatcgt tctcgctttg attttggccc
    12301 tgggcctgac ggcctcagtg caagccaatg tggcaggccc ggcctgggac aaggcaccgg
    12361 acaagatcaa ggacatggca gcgttgcaaa gcggtgccaa gacatttgtc aactattgcc
    12421 tgaattgcca ttcagcggcg ttcatgcgct tcaaccgctt gcaagacatc ggcttgacat
    12481 cggagcagat cagggacaac ctgctggtta ccaatgggaa gcttggtgaa accatgaagg
    12541 cggccatcga tccgcagcag gccaaggctt ggtttggggt gaatccgcct gatctcacgg
    12601 tgatcgcgcg atcccgtgct ggtgctggcg gcacaggtac cgactatctc tactcgtaca
    12661 tgcgtgggtt ctacaaagac gagaccaaac caacgggttg gaacaatcat gtgttcccga
    12721 atgtagccat gccacatgtg ctttgggaaa tgcagtccac aatgacacct gctcagtatg
    12781 atcagaccat gggcgatctt gtgaattact tgcagtggat ggctgagcca gcccaaacgg
    12841 cgcggaaaac cattggcatg tgggttttgg tgtttttggc aggtttgact tttctgactt
    12901 ggcggctcaa tgctgcatat tggaaagaca tcaagtaact gggttcattg atctgcacga
    12961 cctctggtac ggtgcagatt cttcagagtg ggatgcttaa cggcctcccg ctcttttatt
    13021 ttttaggagc ctccaccatg atggtgcttt actcgggcac aacttgtcca ttttcccatc
    13081 gctgtcgttt cgtgttgttc gaaaaaggca tggattttga aattcgggat gttgatcttt
    13141 ataacaaacc cgaagatatc aacgtgatga atccgtatgg tcaagtgcct attctggtcg
    13201 aacgtgattt gatcttgtac gagtctaata tcatcaatga gtacattgac gaacgctttc
    13261 cacatcccca gttaatgccg ggcgatcctg tggatcgggc acgggtaagg ttgtttttgc
    13321 tcaattttga aaaagagctg ttcgcgcatg tgtcaacctt ggagtcgcgt actgccaagt
    13381 ccaatgaaaa agccgtggaa aaggcgcggg cgcatatccg agaccgactc acccagttgg
    13441 ccccggtttt cctcaagaac aagttcatgc tgggcgacgg attttcgatg ctggacgtgg
    13501 ctattgcgcc tttgctgtgg cggctggatt attacggcat cgaacttagt aaaaatgcgg
    13561 ctcccttgct caaatatgca gagcggattt tttctcgccc agcgtacatt gaagcgctga
    13621 cgccttctga aaaagtgatg cgtaaatagg tggcttgatc tatgtcagtt aatcaaaaac
    13681 ctgaggctac ctcaacgcgc ccttatctca ttcgtgcttt gcatgagtgg tgctgtgaca
    13741 atggtttaac tccgtacatc accgttgcag ttgacaactc tgtgcaagtg ccacgggaat
    13801 atgtcaagga cgatgagatc gttttgaaca tcggttttga cgcgaccact gctctaagca
    13861 tgggcaatga gtaccttgag ttcaaggccc gattttcggg cgcagttcgc gacatcatgg
    13921 ttccaatgga tcggatcagc gcgatcttcg cgcgtgaaaa tgggcaggga atggcgtttc
    13981 cgatggttga tcgttctgaa cagaatcggt caagtcgcaa agaaacagag ccacgccctg
    14041 ccgtatcttt ggcgctggcg caaaagaaac tgggtttaaa tggcgacatc gagggtggcg
    14101 atttggacaa cgattccgac accagaccca gcccacaaag ccagggctcg aatccgggtg
    14161 ccaaaccatc gctaacacgt gtcaaatgaa gtcattagcc ggtctttagc ctgaagttct
    14221 ttccccaaaa ggccgagttt ccccaatgaa acccacgcta aatttggttc cgcgttctgt
    14281 ctacgg
//
LOCUS       DAOSLL010000033        14278 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4345_length_14278_cov_4.864164, whole genome shotgun sequence.
ACCESSION   DAOSLL010000033 DAOSLL010000000
VERSION     DAOSLL010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 14278)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14278)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14278
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4345_length_14278_cov_4.864164"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="PLB25_02515"
     CDS             <1..657
                     /locus_tag="PLB25_02515"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HOE40498.1"
                     /translation="NRSGITLVNIGVGPANAKTITDHIAVLRPHAWLMLGHCAGLRNT
                     QSLGDYVLAHGYVREDHVLDEDLPLWVPIPALAEIQVALEASVSEVTRLKGHELKSIM
                     RTGTVASTDNRNWELLPDNGPQRRFSQSRAIALDMESATIAANGFRFRVPYGTLLCVS
                     DKPLHGDIKLPGMANHFYRERVDQHLRIGIRALELLRDQGVDQLHSRKLRSFVEVAFQ
                     "
     gene            819..1088
                     /locus_tag="PLB25_02520"
     CDS             819..1088
                     /locus_tag="PLB25_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40499.1"
                     /translation="MFLTMQDCIDMSDLTEEEILAISEHEGFSEILAIELGSYLVHTP
                     DGKKRIKRMIQDDIVIAQMKGDLQHAAVLKSVLKHYLEHHMEHVG"
     gene            complement(1272..2345)
                     /locus_tag="PLB25_02525"
     CDS             complement(1272..2345)
                     /locus_tag="PLB25_02525"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOE40500.1"
                     /translation="MSERNQSLDALRGLAASLVLVAHASQAYAPVARQHGHDTWLGDA
                     TLGIDIGRTGVVLFFLISGFTVANSLRQSSGGVDGFAVRRFFRLYPLFWLSVLLSVVF
                     FQSAPKLDWLAVAANLTMLPELFGIAQMQGVYWTLETELISYAIAALLLAAGWFNDAR
                     KLAVICGVLLMVFALMMFEVLPSARILQWQMLPHNLAIILWGSLFHLTHGAQKIGTPP
                     PGERALRRITLLTAILLISPSCYALLQYAMHGKPEHLRWGFAYPFAFALFLLTFFWIK
                     KVPHWLVWLGQISYSSYLLHVFVISLLLELLQKSSLAVGWVGVPLFVLMSLLATTAVA
                     NLSFVVLEKPAMRWGKALARRCR"
     gene            complement(2523..3764)
                     /gene="purT"
                     /locus_tag="PLB25_02530"
     CDS             complement(2523..3764)
                     /gene="purT"
                     /locus_tag="PLB25_02530"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HOE40501.1"
                     /translation="MTTIGTPRSPNATKVMLLGSGELGKEVLIALQRLGVETIAVDRY
                     DHAPGHQVAHHARTITMSDPGMLRALIEADQPDLVVPEIEAIATPMLEVLEATGVVRV
                     VPTARAARLTMDREGIRRLAAEELGLPTSRYRFCDSLAELQAAIDGSIAAPGIGYPCI
                     VKPVMSSSGKGQSKISGPAGVAKAWDDAMAGGRVSHGRVIVEGFIDFDYEITLLTVRA
                     KGADGSIETHFCQPIGHIQVNGDYVESWQPQPMHPQALQQARHIARTVTDNLGIGSDG
                     QPSGLGLFGVELFVKGNQVWFSEVSPRPHDTGLVTLTTQVQSEFELHARAILGLPVNT
                     ALRTPGASAVIYGGIDATGIVFDGVDEALRVPNSDLRLFGKPESFVKRRMGVALAFDA
                     DVAVARVNAKLSASCIKPRAS"
     gene            4044..4967
                     /locus_tag="PLB25_02535"
     CDS             4044..4967
                     /locus_tag="PLB25_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE40502.1"
                     /translation="MSESIFSVQGLVKRYGEATVVSDMSFEIAPGECLGVIGPNGAGK
                     TTTIRMCLGLAVPDAGSVTAFGLSMPKDSLAIKAQLGVVSQMDTLDPDFSCAENLLVY
                     GRYFGMKKALIKERIPSLLEFASLTSKADAKPGELSGGMKRRLSLARALVNNPRLLLL
                     DEPTTGLDPQARHLMWERLQLLLQEGKSILLTTHFMDEAERLCHRLLVVDHGRKIAEG
                     APRDLIRQYLEPDVVEVFGSGALALIDSPLKTLAARVEVSGETVFFYTADAKPLLAAL
                     ESNPQLRTLHRPANLEDLFLKLTGRQIREDA"
     gene            4964..5785
                     /locus_tag="PLB25_02540"
     CDS             4964..5785
                     /locus_tag="PLB25_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE40503.1"
                     /translation="MSSPDNNPLNPSIWRAPEISGRWWPVFLRNLLVWRKLAIPSLVG
                     NIAEPLMWLVAFGYGMGALVGQVTVNGTQVPYILFLASGSICMSAMNAASFEALYSAF
                     SRMHVQKTWDGIMNAPVNLDNIVLAEMLWAAFKSLFTVTAILGVMLALGISYSPKLLI
                     AWPVLLGVGITFSCVALIFNALAKGYDFFTYYFTLFLTPMMFLSGIFFPLEQLPPVVR
                     AISQWLPLTNAVDLVRPLFMDQWPTQWLRPVLVLASFAVAGFWIALALTRKRFRG"
     gene            6264..7766
                     /locus_tag="PLB25_02545"
     CDS             6264..7766
                     /locus_tag="PLB25_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOE40504.1"
                     /translation="MNATVSPESPSTLQRSELQAQVVKALQADLPSHALLWNSEDTTP
                     YECDGLTAYRQRPLVVALPETNAQVQAVLRCCHALRVPVVARGAGTGLSGGALPHAMG
                     VTLSLAKFNKIIKVDALSRTATVQCGVRNLAISEAAAPLGLYYAPDPSSQIACTIGGN
                     VAENAGGVHCLKYGLTLHNIVKIKGFTMDGQAVTLGSDALDTPGLDLMGVVIGSEGML
                     LVISEVTVKLVPKPQLARCIMASFDDIRKAGDAVASVIAAGIIPAGLEMMDKPMTAAV
                     EDYVHAGYDLNAEAILLCESDGTPEEVAEEIGRMSDVLRSCGATAIAVSRDEAERLKF
                     WSGRKNAFPASGRISPDYMCMDSTIPRKRLADLLLAIAEMEKKYQLRCANVFHAGDGN
                     LHPLILFDANDADQLHRCEQFGAEILETSVAMGGTVTGEHGVGIEKLNSMCVQFSGAE
                     NEQMFGLKRAFDPLGLLNPGKVIPTLQRCAEYGKMLVRGGQLKHPELPRF"
     gene            7776..8123
                     /locus_tag="PLB25_02550"
     CDS             7776..8123
                     /locus_tag="PLB25_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003818553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HOE40505.1"
                     /translation="MLALRGLAWLLTMQSLGELLSRGLALPFPGPVLGMLLLLVGLRL
                     PLVRAPVAACAGFLLSHLSLLFVPVGVGVMTHLGLLGQYGLRMLAVIVLSTWIGLVVT
                     ALVLRSFKSAPDA"
     gene            8116..8841
                     /locus_tag="PLB25_02555"
     CDS             8116..8841
                     /locus_tag="PLB25_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HOE40506.1"
                     /translation="MPKFVELWVYLSATPLFGLTATLVVYVLAQAVYARLSQASWANP
                     VLWSVLALASLLTVTGVDYPTYFSGAQFIHFLLGPAVVALAWPLWDRRFELLARWRAL
                     LLAALVGGIAASGSAMALGWAVGLPAEVVLSLAPKSVTAPVAMGIADKIGGIPALAAV
                     FAVVTGMVGALSGKFLFGLLGIGSDRAGWIAQGFALGTAAHGIGAARALQVNADAGAY
                     AGLALGLQVLLASLLIPLLFRLF"
     gene            complement(8993..10771)
                     /gene="ptsP"
                     /locus_tag="PLB25_02560"
     CDS             complement(8993..10771)
                     /gene="ptsP"
                     /locus_tag="PLB25_02560"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOE40507.1"
                     /translation="MTFSIHGLAVARGITIGRAVLVASSRLDVAHYFIEPSQVIAEIN
                     RVRDGRDAVVNEIHRLQANIAHMGPKEAPHELTALLDVHLMLLQDEELSLGVKHWIKD
                     RLYNAEWALTSQLEVVARQFDEMEDDYLRERKADLEQITEKVLRAMRGVSSPVFQPQV
                     RTTRKASQQDLLLDDTVDVPLILVAHDLSPADMLQFKQSVFAGFVTDVGGKTSHTAIV
                     ARSLEIPAVVGARWSSQLLRQDDWVIIDGDAGIVVVDPSPIILAEYGFKQRQVELERG
                     RLARLLHTPAVTMDGQKIELLANIEMPDDAAGAVKAGAAGVGLFRSEFLFMGRHGKLP
                     NEDEQYKAYRRAVEGMQGLPVTIRTVDVGADKPLDESLHDGTHLNPALGLRAIRWSLA
                     DPAMFLTQLRAILRAAAHGQVQLLIPMLVHASEIRQTMALIDHARVQLDNRGVAYGPV
                     KVGAMIEIPAAALTLKVFLKYFDFLSIGTNDLIQYTLAIDRADESVSHLYDPTHPAVL
                     RLVANTIAEGRAQGKPVSVCGEMAGDVSLTKLLLGMGLRSFSMHPAQIQAVKQEVLRA
                     DTVKLTPWAESVMAEDEPGRRVVKAG"
     gene            complement(10768..11037)
                     /locus_tag="PLB25_02565"
     CDS             complement(10768..11037)
                     /locus_tag="PLB25_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOE40508.1"
                     /translation="MIKTTTTISNKLGLHARASAKLTKLAGSFPCEVWMSRGDRRVNA
                     KSIMGVMMLAAGQGVQIEIETEGPREQDAMDALLALVRDKFGEGE"
     gene            complement(11006..11452)
                     /locus_tag="PLB25_02570"
     CDS             complement(11006..11452)
                     /locus_tag="PLB25_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HOE40509.1"
                     /translation="MNNGIFIIAHAPLASALRQCVLHVFPDAAADLAVMDVLPNMPTE
                     ETLVSARLAVAMLNRPQTLVLTDMFGATPCNVAQKVVDGLNSRLVAGVNLPMLMRAIT
                     YRNEPLDALVARALAGGTQGVMQVAVTAPQNQIRKSHDKNHHDHQQ"
     gene            complement(11524..12756)
                     /locus_tag="PLB25_02575"
     CDS             complement(11524..12756)
                     /locus_tag="PLB25_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007847778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE40510.1"
                     /translation="MGTMPRRTAVLVFLAFAAAYFLSALIRAITATLSPTLTQEFALN
                     ARDLGLLAGGYFLGFAATQLPLGTWLDRFGPKRVVLSLLTVAVVGCLAFAMATSFSGL
                     LLARILSGVGVSACLMAPLTGYRRWFAAVSQMRANSWMLMAGSLGMVASTLPVQWLMP
                     LIGWRPLFWGLALLVVLAMLLIGWQVPAWSQAGPVADSAGPTASRTGSYAEVWRNPYF
                     RKMAPMGFFIYGGLVAMQTLWVTPWLIRVNGWTALQAAGGLFWLNVAMLITFWGWGLV
                     NPWLTGRGYHADRLISFGMPFSFFLLALIIIAGSAMDIWAGAAWALYCMGCTCISLAH
                     PAVALAFPATLAGRALSAYNLLIFSGVFVVQWGVGLMMDGFQAFGWSPVQGFQGAMGV
                     YLLCTVLSYIYFLTAKKP"
     gene            complement(12778..13482)
                     /locus_tag="PLB25_02580"
     CDS             complement(12778..13482)
                     /locus_tag="PLB25_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HOE40511.1"
                     /translation="MKRRHFTATLVCVAASPLAAIHPGALAQGADALSNLQVSQGLGV
                     ALEKGALSAIKLLGRTDGFLGNDKVRIPLPGVLQDASKLLRSFGQGARLDELVNAMNR
                     AAEAAVPLAQATLVAAIKAMNFQDAKAILGGGDTSVTRFFADKTREPLGLKFLPIVKQ
                     TTARVNLAEKYNQLAGKAAGMGLLKGDQVNVERYVTGKTLDGLYFMIGEEEKKIRQDP
                     VGTGSSLLQKVFGSLK"
     gene            complement(13580..14032)
                     /locus_tag="PLB25_02585"
     CDS             complement(13580..14032)
                     /locus_tag="PLB25_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HOE40512.1"
                     /translation="MKKLSAALLAVVASCYVVPAFAQFAKPEDAIKYRKSALFVMQQN
                     FGRVAAMASSKAPFDAKIAVESAAVAEFVGTLPWAGFAAGTDKGDTKAKPEIWTDKAK
                     FDDYAKKLQAELTKLSAAAKTGSLDNVKVAVNAAGGACKSCHDDFRAK"
     gene            14134..>14278
                     /locus_tag="PLB25_02590"
     CDS             14134..>14278
                     /locus_tag="PLB25_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome B"
                     /protein_id="HOE40513.1"
                     /translation="MKAVVRVWDAPTRLFHWALALCVIGLVATAQIGGTAMQWHFRFG
                     YTVL"
BASE COUNT         2903 a         4289 c         4269 g         2817 t
ORIGIN      
        1 aaccgcagcg gcatcaccct ggtgaacatc ggcgttggcc cggccaatgc caagaccatc
       61 accgaccaca tcgccgtgct gcgcccgcat gcctggctga tgctcgggca ctgcgccggc
      121 ctgcgcaaca cgcaaagtct gggcgactat gtgctcgcgc acggctatgt gcgcgaggac
      181 catgtgctcg acgaagattt gccgctgtgg gtgccgattc cggcgctggc cgaaatccag
      241 gtggcgctcg aagcctcggt gtccgaagtc acccggctca aaggccatga actcaagagc
      301 atcatgcgca ccggcaccgt ggccagcacc gacaaccgca actgggagct gttgcccgac
      361 aacggcccgc agcgccgctt cagccaaagc cgtgcgattg cgctggacat ggaatcggcg
      421 acgattgctg ccaatggttt cagatttcgc gtgccctatg gcaccttgct gtgtgtcagc
      481 gacaagccgc tgcacggcga tatcaaactg cccggcatgg ccaatcactt ttaccgtgag
      541 cgggtcgatc agcatttgcg cattggcata cgggcgctgg aattgctgcg cgatcagggt
      601 gttgatcagc ttcacagccg caagctgcgc agttttgtcg aagtagcgtt tcagtaaaac
      661 cgctcgggtc aagctttgac gacccgcctt cgccggctgt acaaatatga tttgtcaagc
      721 aatgaaagcc tgtttaccat tagcaacagg caccttcgcc agacccacgg ccgtgccaca
      781 gaccatcgta agttcaactt aaccaaggag caagtgccat gtttttgacc atgcaagact
      841 gcatagacat gtcagacctg accgaggaag agattctcgc catctctgaa cacgaggggt
      901 tttccgagat acttgccatc gaacttggca gctatctggt gcatacgcct gatggcaaga
      961 agcgcatcaa acgcatgatt caggacgata tcgtgatcgc gcagatgaaa ggcgatctgc
     1021 agcacgccgc cgtgctaaag agtgtgctca agcactatct tgagcatcac atggaacatg
     1081 tcggctgagc atctgaccga tcttgcgctc aggcatgacc tcggagtccg gccagccgag
     1141 cctcttcccg gtcacgcgag gcggatgccg atcccaatct ggctcgccgc agcttgcatc
     1201 aacctcaatg acttgcgcca cgacgtggtt gggcttcgtg gcgctgctga cgacggtgtc
     1261 gaggccgact atcagcgaca ccggcgggcc agcgctttgc cccagcgcat cgcgggcttt
     1321 tcaagcacga caaaagacag attggcaact gccgttgtgg ccagcaagga catcaggaca
     1381 aacagcggca cgccgaccca gcccactgcc aggctgcttt tttgcagcag ttcgagcaac
     1441 agcgagatga caaaaacatg cagcaaatag cttgaatagc tgatctgccc gagccagacc
     1501 agccagtgtg gcaccttctt gatccagaaa aaggtgagca gaaagagcgc gaacgcaaac
     1561 ggataggcaa acccccagcg caggtgctct ggcttgccgt gcatggcgta ttgcaacaat
     1621 gcgtagcacg aaggactaat cagcaatatc gccgtcagca gcgtgatgcg gcgcagcgca
     1681 cgttcgccgg gcggcggtgt gccgatcttt tgcgcgccgt gggtcaggtg aaacaggctt
     1741 ccccacagga tgattgccag gttatggggc aacatctgcc actgcagaat ccgggctgac
     1801 ggcagcacct caaacatcat cagcgcaaaa accatcagca aaacgccgca gataacagcc
     1861 agctttcgcg cgtcattgaa ccagccagcg gcaagcagca gcgcggcaat cgcataggag
     1921 atgagttcgg tttccagcgt ccagtaaaca ccttgcatct gcgcaatgcc aaacagttcg
     1981 ggcagcatgg tcaggttggc ggcaaccgcc aaccaatcaa gctttggcgc tgactggaag
     2041 aaaaccacac tgagcagcac tgaaagccaa aacagcggat agagccgaaa aaaccgccga
     2101 accgcaaagc cgtcaacgcc gcctgagctc tggcgcaggc tgttggccac cgtaaagccg
     2161 ctgatcagga aaaacagcac aaccccggta cgcccgatgt cgatgcccag cgtggcatcg
     2221 ccaagccagg tgtcatggcc atgttggcgc gccaccggcg catacgcctg gctggcatgc
     2281 gctaccagca ccagcgacgc ggccagcccc ctgagggcat ccaggctctg gtttctctca
     2341 gacaaactaa ctgctcgcac gtcatcctgc atgttgatcc ttggtctatc ttcagatggc
     2401 gggttgtggc tgcacccgca tccggctgcg gcgctagacc tgcttgccag aaataatgca
     2461 aaaatcgcct gtagccctta tgtttattgc gctggcagct attgtttttg atctttttag
     2521 aatcagcttg cgcgtggctt tatgcacgac gcgctcagct ttgcgttgac ccgggccaca
     2581 gccacatcgg catcaaacgc cagcgccacg cccatgcggc gcttgacaaa actctctggc
     2641 ttgccaaaca ggcgcaaatc tgaattgggc acccgcagcg cctcgtcaac gccgtcaaac
     2701 acaatcccgg ttgcgtcaat gccaccataa atcaccgcac tggcaccagg tgtgcgcagc
     2761 gcggtgttga ccggcaagcc caaaatggcg cgggcatgca gctcaaattc gctctgcacc
     2821 tgtgtggtga gggtcaccag gccggtgtca tgcgggcgcg gcgacacttc actgaaccag
     2881 acctgattgc ccttgacgaa cagctccacg ccaaacaaac ccagacccga aggctggccg
     2941 tccgagccaa tgccaaggtt atctgtcacc gtcctggcga tgtgccgggc ctgttgcagc
     3001 gcttgcgggt gcatcggctg cggctgccag ctttccacat agtcgccgtt gacctgaatg
     3061 tggccaatcg gctggcaaaa gtgcgtctca attgagccgt cggcaccctt ggcccgcacc
     3121 gtcagcaggg tgatctcata gtcaaagtca atgaacccct cgacgatgac gcgaccgtgg
     3181 ctgacccgcc cgcccgccat cgcatcatcc caggcctttg ccacaccggc cgggccgctg
     3241 atcttgcttt ggcctttgcc cgagctgctc atcaccggct tgacgatgca ggggtagcca
     3301 atgcccggcg cggcaatgct gccatcaatg gccgcctgca gttcggccag cgaatcgcaa
     3361 aaccggtagc ggctggttgg caagccaagc tcttcggcgg ccaggcgacg gatgccttcg
     3421 cgatccatcg tcaggcgcgc ggcgcgggca gtcggcacca cccgcacgac accagtggct
     3481 tccagcactt caagcatcgg ggtggcaatg gcctcaatct cgggcaccac caggtcgggc
     3541 tggtcggctt cgatcagcgc acgcaacatg ccagggtcgc tcatggtgat ggtgcgggcg
     3601 tggtgcgcca cctggtgccc cggtgcatgg tcgtagcgat caaccgcaat cgtttcgaca
     3661 cccaggcgct gcagtgcaat cagcacctct ttgccgagtt cgccgctgcc cagcagcatg
     3721 actttggttg cattgggaga ccggggtgtg ccaatagttg tcatgggttt aggcgttgcc
     3781 gcgtccgaac agggttgggg ataagaggcc tcattttagg gatgcagggc aagtggcagc
     3841 gcccgctgcc caccagcacc accaggggct ggccatgacc cagattctca atgataaaaa
     3901 agggctccag cgcttgatgg cattgcgctg agtgctatca aactaattgt gacgtgcaga
     3961 atttggcaag cgcggccaga acctgcttgc gcggtaaacc cgagtgcggc ctgaggcggc
     4021 ggcggctggg tgacaattca cccatgagtg aatccatctt ttcagtacaa ggcctggtca
     4081 agcgctatgg cgaagccacg gtggtcagtg acatgtcgtt cgagatcgcg ccgggcgaat
     4141 gcctgggcgt gatcggcccc aatggtgccg gcaagaccac caccatccgc atgtgcctgg
     4201 ggctggcggt acccgacgct ggcagcgtca cggcttttgg cctgtccatg ccgaaggact
     4261 cgctggccat caaggcgcaa ctcggtgtgg tcagccagat ggacacgctc gacccggact
     4321 tcagttgcgc cgagaacctg ctggtctatg gccgctactt tggcatgaag aaagcactca
     4381 tcaaggaacg tattccatcg ttgctggagt tcgcatcact caccagcaag gccgacgcaa
     4441 agccgggcga attgtcgggc ggcatgaagc gccgactcag tctggcacgc gcactggtga
     4501 acaacccgcg cctgctgctg cttgacgagc cgaccaccgg gctggatccg caggccaggc
     4561 acctgatgtg ggagcggctg cagttgctgt tgcaagaagg caagtcgatc ttgctgacca
     4621 cccacttcat ggacgaagcg gagcgccttt gccaccgcct gttggtggtg gaccatggcc
     4681 gcaaaattgc cgaaggggca ccccgtgacc tgatcaggca gtatctggaa cctgatgtgg
     4741 ttgaggtttt tggcagcggc gcgctggcgt tgattgattc gcccctcaaa accctggcgg
     4801 cacgggtcga ggtcagcggt gaaacggtgt ttttctacac tgctgatgcc aaaccgctgc
     4861 tggcggcgct ggagtccaac ccgcaattgc gcacgctgca ccgaccggcc aatctggaag
     4921 acctgttcct taaactgacc ggccgtcaaa tccgggagga cgcatgagta gccctgacaa
     4981 caaccccctg aacccgtcaa tctggcgcgc acccgagata tccgggcgct ggtggccggt
     5041 ttttctgcgc aacctgctgg tctggcgcaa gctggccatt cccagcctgg tcggcaacat
     5101 cgccgagccg ctgatgtggc tggtggcttt tggctacggc atgggcgcgt tggtcgggca
     5161 ggtcacggtc aacggcaccc aggtgcccta catcctgttt ctggccagcg gctcgatctg
     5221 catgagcgcc atgaacgcgg cgagttttga ggcactgtac tcggcattca gtcgcatgca
     5281 tgtgcagaaa acgtgggacg gcatcatgaa cgcgccggtc aacctggaca acatcgtgct
     5341 cgccgagatg ctgtgggccg ccttcaagtc gctgtttacc gtgaccgcca ttctgggcgt
     5401 gatgctggcg ttgggcatca gctactcacc aaagctgctg attgcctggc cggtgctgct
     5461 cggggtcggc atcactttca gttgcgtggc gctgatcttc aacgcgctgg ccaagggcta
     5521 tgactttttc acctactact tcacgctgtt cttgacaccg atgatgttcc tcagcggcat
     5581 tttttttccg ctggagcaac tgccaccagt cgtgcgcgcc atctcgcaat ggctgccgct
     5641 gaccaatgcg gtcgatctgg tgcgcccgct gttcatggac caatggccaa cccaatggct
     5701 gcgaccggtg ctggtgctgg cgagttttgc cgtggcgggc ttctggatag cgctggcact
     5761 gacccgcaaa cggtttcgtg ggtagcccac attactactg aatagctagc tatcggcgca
     5821 cgccccactg gggctagcag ccatttttac ctatgacatc gagggcggga atcgtctcca
     5881 gcgagaccgc tgcaggcggc gcgcaaccga tgtgcgatag gtctgagagg ctgctggcgc
     5941 ttcatcctgg acttttcagt tgggcgcttg ctttcatctg taagtcctca tgcgtattgg
     6001 ctttttcagc atcgatggcg tgcacccgtg gggtgaaggc gctacccgcc caattgaggc
     6061 atcctggatc aggtcgatca atacctgggg cagccgtgac cgatgtgttc cagcacgaca
     6121 gcgccgacca ccaatatggc agtgctggcg gcgctcagcg atgcagtcag cggttttcaa
     6181 aagagcattt aagtttcttc acaccgcgcc gggtcaattt ggcttagagt gccatccacg
     6241 ccatctgcca caggattgaa accatgaacg ccaccgtgtc ccccgagtcg cccagtacgc
     6301 tacaaagatc agagctgcaa gcgcaagtag tgaaagcgct acaggccgat ttgccatctc
     6361 atgcgctgct ctggaacagc gaagacacga caccctatga atgcgacggc ctgaccgcct
     6421 accgccagcg tccgctggtg gtggcgctgc ccgagaccaa cgcgcaggtg caggcggtgc
     6481 tgcgctgctg ccatgccttg cgggtgccgg tggtggcgcg cggggctggc accggcctgt
     6541 cgggtggcgc gttgccgcat gccatgggcg tgacgctgtc gctggccaag ttcaacaaaa
     6601 tcatcaaggt cgatgcgctg tcgcgcacgg cgacggtgca atgcggggtg cgcaacctgg
     6661 ccatcagcga ggccgcagca ccgctgggcc tgtattacgc gcccgacccg agcagccaga
     6721 ttgcctgcac cattggcggc aacgtggcag aaaacgcagg cggtgtgcat tgcctgaaat
     6781 atggcttgac gctgcacaac atcgtcaaga tcaagggctt cacgatggat gggcaggctg
     6841 tcacgctggg ctctgacgcg ctcgacacgc ccgggctcga cttgatgggt gtggtgattg
     6901 gcagtgaggg catgctgctg gtgatttcag aagtgaccgt caaactggtc cccaaaccgc
     6961 aactggcgcg ctgcatcatg gccagttttg acgacatccg caaggccggt gatgcggtgg
     7021 ccagcgtgat tgcggcaggc atcattccgg ccgggctgga aatgatggac aaacccatga
     7081 cggccgcggt ggaagactac gtgcatgcgg gttacgacct gaacgccgag gcgattttgc
     7141 tgtgcgaaag cgacggcacg cctgaagaag tggcagaaga aattggccgc atgagcgatg
     7201 tgctgcggtc ttgcggtgcc accgcgattg ccgtcagccg cgacgaggcc gagcggctga
     7261 agttctggag cggacgcaaa aatgcctttc cggcttccgg gcgcatcagc ccggactaca
     7321 tgtgcatgga cagcaccatt ccgcgcaaac gcctggccga tttgctgctg gccattgcgg
     7381 agatggagaa aaaataccag ttgcgctgcg ccaatgtgtt ccacgcaggc gacggcaacc
     7441 tgcacccgct gattctgttt gatgccaacg atgccgatca actgcaccgc tgtgagcagt
     7501 ttggtgccga gatactggaa accagcgtgg cgatgggtgg caccgtgacc ggcgagcatg
     7561 gcgttggcat tgaaaaactc aacagcatgt gcgtgcagtt cagcggggca gaaaacgagc
     7621 agatgtttgg gctcaagcgg gcctttgacc ccttgggcct gctcaacccc ggcaaggtca
     7681 tcccgacgct gcagcgctgt gccgaatacg gcaagatgct ggtgcgtggc gggcaactca
     7741 aacaccccga attgccacgt ttttaatagc acgagatgct tgctttacgg gggctggcat
     7801 ggcttttgac catgcagtca ttgggtgaac tgctgtcgcg cgggctggcc ctgccgtttc
     7861 ccgggccggt gctgggcatg ctgctgttgt tggtgggctt gcgcctgccg ctggtgcgcg
     7921 caccggtggc ggcctgcgcc ggcttcttgc tgtcgcattt gtccttgctg ttcgtgccgg
     7981 tgggcgttgg tgtcatgaca cacctgggct tgctcggcca atacggcctg cgcatgctgg
     8041 cggtgatcgt gctgtccacc tggatcggac tggtggtgac ggcgctggtg ttgcggtctt
     8101 tcaaaagtgc ccccgatgcc taaatttgtc gaactctggg tttacctttc tgccacaccg
     8161 ttgtttggcc tgacggcaac gctggttgtt tatgtgctgg cgcaggcggt ttacgcccgg
     8221 ttgtcgcagg cctcatgggc caatccggtg ctgtggtcgg tgctggccct ggccagcctg
     8281 ctgacggtga ccggcgtgga ctatccgacc tatttctcag gcgcgcagtt catccacttt
     8341 ttgctcgggc cggccgttgt cgccctggcc tggccgctgt gggaccgccg cttcgagctg
     8401 ctggcgcgct ggcgtgcctt gctgctggct gcgctggtcg gcggcattgc ggccagcggc
     8461 tcggccatgg cactgggctg ggcggtcggc ctgccagccg aagtcgtcct gtcgctggca
     8521 cccaaatcag tcaccgcgcc ggtggccatg ggcattgccg acaagattgg cggcattccg
     8581 gcgctggctg cggtgttcgc cgtggtgacc ggcatggtag gggcactgag cggcaaattc
     8641 ctgtttggcc tgctcggcat tggcagcgac cgcgcgggct ggatcgcaca gggttttgca
     8701 ctgggcaccg ctgcgcacgg cattggcgca gcgcgcgcct tgcaggtcaa tgccgatgcc
     8761 ggggcgtatg ccggcctggc gcttggcctg caggtgctgt tggcatcgtt gttgataccg
     8821 ttgctgtttc ggttgttctg atctggttga ggcaatgact caagccattg ctgcatgagc
     8881 cttgattgct cattgttgtt ctcagatcat gcgtgtccag acaccctggc actgaccttg
     8941 ccaacgcaag gcaaggcagg ttgaccacga cctggatggc ttggaaatgg cgtcagccgg
     9001 ctttgacaac ccgtcggcca ggttcgtctt ctgccatcac cgactcggcc cagggcgtga
     9061 gtttgacggt gtcagcccgc aaaacctcct gtttgaccgc ctggatttgc gccggatgca
     9121 tggaaaagct gcgcaagccc atgcccagca gcaatttggt caagctcacg tcacccgcca
     9181 tttcaccgca aacgctgact ggcttgcctt gggcacggcc ctcggcaatg gtgttggcca
     9241 ccagtcgcag cacggccgga tgggtcgggt catacaggtg cgacaccgac tcgtcagcgc
     9301 ggtcaatcgc cagcgtgtac tggatcaggt cgttggtgcc gatcgagaga aaatcaaaat
     9361 acttgagaaa caccttgagc gtgagcgccg ccgccggtat ctcgatcatg gcaccgacct
     9421 tgaccgggcc ataggccacg ccccggttgt ccaactgcac gcgggcatgg tcaatcaagg
     9481 ccatggtctg gcgaatctcg ctggcatgca ccagcatcgg gatcagcaac tggacctggc
     9541 catgcgcagc agcacgcaaa atggcgcgca actgggtcaa aaacattgcc gggtcggcca
     9601 ggctccagcg gatcgcacgc aggcccaggg ccggattgag gtgagttccg tcgtgcaggc
     9661 tttcatcaag cggcttgtca gcaccaacat caacggtgcg aatggtgacc ggcaaaccct
     9721 gcatgccctc gaccgcacgc cggtatgcct tgtattgctc gtcttcgttg ggcagtttgc
     9781 catggcgccc catgaacagg aactcgctgc gaaacaggcc gacaccggcc gcaccggcct
     9841 tgacggcacc cgccgcgtcg tcgggcattt cgatattggc cagcagttcg atcttttgcc
     9901 cgtccatggt gacagccggg gtgtgcagca ggcgggccag acgaccgcgc tcgagttcga
     9961 cctggcgttg cttgaagcca tattcggcca ggatgatggg cgatggatcg accaccacga
    10021 tgccggcatc gccgtcgatg atgacccaat cgtcctggcg cagcagttgg ctgctccagc
    10081 gcgccccgac caccgccggt atctccaggc tgcgcgcgac gatggcagtg tgcgaggtct
    10141 tgccacccac atcggtgaca aacccggcaa acacgctttg cttgaactgc agcatgtcgg
    10201 ccggcgacag gtcgtgcgcg accagaatca gcggcacatc aacggtgtca tcgagcagca
    10261 aatcctgctg cgaagctttt cgggtggtgc gcacctgcgg ttgaaacacg ggcgaagaca
    10321 cgccacgcat ggcacgcagg actttttcgg tgatctgctc caggtcggcc ttgcgctcgc
    10381 gcaggtagtc gtcttccatt tcgtcaaact ggcgtgcgac aacctcgagt tggctggtta
    10441 acgcccattc ggcgttgtaa aggcggtcct tgatccagtg cttgacaccc aggctcagtt
    10501 cctcgtcttg cagcagcatc aggtgcacgt cgagcaaggc ggtcagttcg tgcggtgcct
    10561 ctttggggcc catatgggca atgttggcct gcaggcggtg gatttcgttg accactgcat
    10621 cacggccatc acgcacccgg ttgatttcag cgataacctg cgacggctcg atgaaatagt
    10681 gcgcgacatc gagtctgctt gatgccacca gcaccgcgcg gccaatggta atgccccgcg
    10741 caaccgccag gccgtgaatg gagaatgtca ctcaccttct ccaaacttgt cacgcaccag
    10801 cgccagcaag gcatccatgg catcttgctc gcgcgggccc tcggtctcga tttcaatctg
    10861 cacgccctgg ccagcggcca gcatcatcac gcccatgatg ctcttggcat tgacgcgccg
    10921 atcgccgcgc gacatccaga cttcacaggg gaaactgccc gccagcttgg tcagtttggc
    10981 ggaggcacgg gcgtgcagcc cgagcttatt gctgatggtc gtggtggttt ttatcatgtg
    11041 atttccgaat ctggttttgg ggtgccgtca ctgcgacttg catgacaccc tgggttccac
    11101 cggccagggc acgggccacc agcgcatcca gtggctcatt gcggtacgtg atggcgcgca
    11161 tcagcatcgg caggttgacc ccggcgacca gtcgggaatt caggccatcg accacttttt
    11221 gcgcaacatt gcacggggtg gcaccaaaca tgtcggtcag caccagggtt tggggtcggt
    11281 tgagcatggc cacggccagg cgcgcgctga ccagcgtttc ctcagtaggc atgttgggca
    11341 gcacgtccat caccgccaga tcagcggcgg catccgggaa gacatgcagc acacattggc
    11401 gcaaagcact ggccaatggc gcgtgggcga tgatgaaaat accgttgttc atgttttctt
    11461 ggggatgcca ccatgaatca gccgtggcag acaaagcgtt tgaaggaaga acccagagcg
    11521 cgattatggc ttttttgcgg tcaggaaata gatataggaa agcaccgtgc acagcaaata
    11581 gacacccatc gcaccctgaa aaccctgcac cggcgaccat ccaaacgcct gaaaaccatc
    11641 catcatcagg ccgacccccc actgcacgac aaaaacacct gaaaaaatca acaggttgta
    11701 ggccgacaac gcgcgcccgg cgagtgttgc cggaaacgcc aacgcaaccg cagggtgcgc
    11761 cagggaaatg caggtgcatc ccatgcaata caaggcccag gcggctccag cccatatatc
    11821 cattgcgctg ccagctatta ttatcagagc aagtagaaag aagctgaagg gcatgccaaa
    11881 actgatcaaa cggtctgcat ggtagccacg gccagttagc caggggttga ccagacccca
    11941 gccccaaaag gtgatcaaca tcgcaacatt gagccaaaac aacccgcccg cagcctgcag
    12001 cgcggtccag ccgttgacgc gtatcagcca cggcgtaacc cacagcgtct gcatggcgac
    12061 cagtccacca tagatgaaaa agcccatcgg tgccattttg cggaagtagg ggttgcgcca
    12121 gacctcggca taactgccgg tgcgggaagc ggtcggcccc gctgaatctg caacaggccc
    12181 ggcctgcgac caggctggca cttgccaccc aatcaacagc atggccaata caaccagcag
    12241 cgccaggccc cagaacaagg gccgccagcc gatcaacggc atcagccatt gcactggcaa
    12301 ggtggaggcc accatgccca gcgagccggc catcagcatc caggagttgg cgcgcatttg
    12361 ggaaacagcc gcaaaccagc gccggtatcc ggtcagcgga gccatcaggc aagcactgac
    12421 gccaacacca ctcaggatgc gcgccagcaa cagcccgctg aaactggtcg ccatggcgaa
    12481 ggcaaggcag cccaccacag ccaccgtcag caggctcaaa accacccttt tcggaccaaa
    12541 tcgatcaagc caggtaccca gaggcaattg ggtcgccgca aaacccagaa aatagccgcc
    12601 agccagcaga cccagatcgc gggcgttgag ggcaaattct tgcgtcaggg taggtgacag
    12661 cgtggccgtg atcgcccgga tcaacgctga cagaaagtag gcagcagcaa acgcgaggaa
    12721 caccagaaca gcggtgcgcc ggggcatggt gcccatggac agctaaggca cgccaagcta
    12781 cttcagggag ccaaacactt tttgcagcag ggagctgccg gtacccactg ggtcctggcg
    12841 tatcttcttc tcttcttcgc caatcatgaa atacaggcca tccagggttt tgccggtgac
    12901 atagcgctca acattgacct gatcaccctt gagcaaaccc atgccggcag ccttgccggc
    12961 aagctggttg tatttttcag ccagattcac gcgggccgtg gtttgtttga cgattggcag
    13021 aaacttcaga cccagcggct cgcgtgtctt gtcagcaaag aaccgggtga ccgaagtgtc
    13081 gccgccgcca agaatggctt tggcatcctg aaagttcatg gctttgatgg ccgcaaccag
    13141 cgtggcctgg gccagcggca cagcagcctc ggcggcacgg ttcatcgcat tgaccagttc
    13201 atcaagtcgt gcgccctggc caaaactgcg cagcagtttc gacgcatctt gcaaaacccc
    13261 tggcaagggt atgcggacct tgtcgttgcc caggaagcca tcggtacgtc ccaacagctt
    13321 gatggcagag agtgcgcctt tttccagcgc taccccgagc ccttgcgaca cctgaaggtt
    13381 ggagagtgcg tctgcgccct gcgccaatgc gccagggtga atagctgcca atgggctggc
    13441 agcgacacaa accaatgttg cggtgaaatg gcggcgcttc atgatgttgc cttgaggaaa
    13501 gttggacccg gcatcttaac gcgcaccaga tcgacgctca cgccagcgtg gcgcagcaaa
    13561 cccattgact acttggcaac tacttggcgc ggaaatcgtc gtgacaggac ttgcaggccc
    13621 cacccgcagc gttcaccgcc accttgacgt tgtcaagact gcctgttttg gcagcagcag
    13681 agagtttggt caactcggct tgcaattttt ttgcatagtc gtcgaacttg gctttgtcag
    13741 tccagatttc cggcttggcc ttggtgtcgc ccttgtcggt gcccgcggca aaaccggccc
    13801 acggaagcgt gccgacaaat tcggcaacag ccgcgctttc aaccgcaatt tttgcgtcaa
    13861 agggtgcttt gctggatgcc atggcagcga cgcgaccaaa attttgctgc atcacaaaca
    13921 acgcactttt gcggtatttg atggcatctt cgggcttggc aaattgggca aacgctggca
    13981 ccacatagca gcttgcaaca accgcgagga gagcagcaga aagtttcttc atgggattcc
    14041 gatcaaagtt aaactaaaaa aagaggtttt cggggattca gagtctattg tgatttgaat
    14101 ctttgtgcag gttgcttttt ggcaaagggt tctatgaaag ctgttgttcg cgtatgggac
    14161 gctcctaccc ggctatttca ctgggccctg gcgctgtgtg tcattggcct ggtggcaact
    14221 gcccaaattg gtggaactgc catgcaatgg cattttcgat ttgggtatac ggtgctga
//
LOCUS       DAOSLL010000034        14160 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4393_length_14160_cov_4.590925, whole genome shotgun sequence.
ACCESSION   DAOSLL010000034 DAOSLL010000000
VERSION     DAOSLL010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 14160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14160
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4393_length_14160_cov_4.590925"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1535)
                     /locus_tag="PLB25_02595"
     CDS             complement(<1..1535)
                     /locus_tag="PLB25_02595"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE40514.1"
                     /translation="MIDKNPFEIARETLKQLTVRKLAPTPINYQRLYSEIAGQPIEPA
                     FPADRLRDIAVALPTRTPGQQKQRGLLESAINQLNWDGVKSALMAYGGFSPPAPDPGK
                     SVNLALAEPAKPVITMIDPAESVPLTAPALTADFFSQIARMVEYALPALGQDDERFLE
                     QTQTLVKALRQPNADAVAVKQMLASYGHRLSFAAEDQAEIKNVLLKLLHLIIENISHL
                     SLDDGWLKGQVDALMNVCVPPLTLRRLDDVEQRLKDVIFKQTEAKSRSVQAHEEMRQM
                     LATFVDRLASMSESTGSFHDKLEESARLIEQARTITDIAPVLKDVVGATRDMAHDSQS
                     ARDELQNMKKRAEATEAELTKLHQELDRVSAQARHDPLTGALNRNGLDEAVRREISKV
                     KRLETPLCMALLDIDNFKKLNDTLGHATGDVALAHLAGVAREVMRPQDTLARYGGEEF
                     VLLLPDTTLEKGIEAMTRLQRELTKRFFLAGTEKVLITFSAGVAQFAPDESGQDAIKR
                     ADQA"
     gene            complement(1603..4197)
                     /gene="gyrB"
                     /locus_tag="PLB25_02600"
     CDS             complement(1603..4197)
                     /gene="gyrB"
                     /locus_tag="PLB25_02600"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HOE40515.1"
                     /translation="MTEENKPVLPIEDTVRTGDDNADSSNFQPTIDAHQAGASEAYGE
                     GSIQILEGLEAVRKRPGMYIGDTSDGTGLHHLVFEVVDNSIDESLAGHCDDILVTIHT
                     DNSVSVVDNGRGIPTGVKMDDKHDPKRSAAEIALTELHAGGKFNQNSYKVSGGLHGVG
                     VSCVNALSKMLRLTIRRDGKVHVMEFCKGFVQNRLIETVNGVEVSPMKVIGQTEHRGT
                     EVHFLPDLEIFQTNTDFHYEILSKRLRELSFLNNGVKIRLKDERTGKEDDFSGAGGVK
                     GFVNFINKGKTILNPNIFHALGDRQSDQNTNIGVEVAMQWNSGYNEQVLCFTNNIPQR
                     DGGTHLTGLRAAMTRVINKYIDDTEIAKKAKVEVTGDDMREGLTCVLSVKVPEPKFSS
                     QTKDKLVSSEVRGPVEDIVSKLLFDYLQERPADAKIIVGKIVEAARAREAARKARDLT
                     RRKGVLDGMGLPGKLADCQEKDPALCEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKI
                     LNVEKARYEKLLTSNEILTLITALGTGIGKAGGSTGNDDFDVAKLRYHRIIIMTDADV
                     DGAHIRTLLLTFFYRQMSELVERGHIYIAQPPLYKVKAGKEELYLQGQTDLENFLLRI
                     ALVNASVFTGGQAAATLAGDGLTELARKHQLAQAVIARLGNFMDVSALRAIADGVALN
                     LDTVADAEVSAAAMQAFLPDAEVAGEFDVRSDKPILRISRRLHGNIKSSVLTQDFVHG
                     ADYAALNEAAKTFKGLLGEGAKVMRGEGERQKEQKVGDFREAMTWLIGQAEHATSRQR
                     YKGLGEMNPEQLWETTMDPTVRTLLRVQIDDAIEADRVFTMLMGDEVEPRREFIETNA
                     LRAGNIDI"
     gene            complement(4344..5450)
                     /gene="dnaN"
                     /locus_tag="PLB25_02605"
     CDS             complement(4344..5450)
                     /gene="dnaN"
                     /locus_tag="PLB25_02605"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HOE40516.1"
                     /translation="MIVLKDTQDKVLAVLQAVSGIVERRHTLPILANVLIRKTGQKLL
                     FTTSDLEIQIRTSAELGGDDGDFNTTVGARKLIDILRSMPADQVVSLESSAAKLILKG
                     GKSRFTLQTMPAEDFPLVQESANFGPVFSVPQKTLKGLLSQVSFAMAVHDIRYYLNGI
                     LFVAEGPQLSLVATDGHRLAFASSTLEVEVPKQEVILPRKTVLELQRLLSDAEGAIDM
                     QFANNQAKFSFGGMEFVSKLVEGKFPDYNRVIPRNHQNSIILGRAALLSTLQRTAILT
                     SDKFKGVRLNIDPGTLRVAANNAEQEEAVDELDIDYSGDSIEIGFNVTYLIDALSNMS
                     QEMIKIEISDGNSSALLTIPEIDHFKYVVMPMRI"
     gene            complement(5567..6973)
                     /gene="dnaA"
                     /locus_tag="PLB25_02610"
     CDS             complement(5567..6973)
                     /gene="dnaA"
                     /locus_tag="PLB25_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HOE40517.1"
                     /translation="MIEGEPPLPEAEQAIPNPDFWQACIDQLSQELPEQQFQTWIKPL
                     TAQVADDLSRVTLFVANRFKLDWIRAQYSGRLALLMERLTGQPVQIELAIAPREASGR
                     RPLLHQSGASQADVTTSADTTPANEPLKSRLNTALTFDALVEGSANRMARAAAMHVAG
                     MPGHLYNPLFIYGGVGLGKTHLMHAVGNQLISERPNAKVLYIHAEQFVTDVVKAYQRK
                     AFDEFKERYHSLDLLLIDDVQFFANKERTQEEFFNAFEALLARKSHIVMTSDTYPKGL
                     ADIHERLVSRFDSGLTVAIEPPELEMRVAILINKAHVEGIVMPEEVAFFVAKNVRSNV
                     RELEGALRKILAYSRFNQKEISIMLAREALRDLLSIQNRQISVENIQKTVADFYKIKI
                     ADMYSKKRPASIARPRQIAMYLAKELTQKSLPEIGELFGGRDHTTVLHAVRKIGGERQ
                     QMTELNQQLHVLEQTLKG"
     gene            complement(7359..10784)
                     /locus_tag="PLB25_02615"
     CDS             complement(7359..10784)
                     /locus_tag="PLB25_02615"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOE40518.1"
                     /translation="MDRPVLRYLIALAKGLVCLFAFAAAGAQEVEQTIRQADQFFNDR
                     EFALALKHYQQASSRIDASSPLNAEVINNIAAAHMAQGEMAAFGHSFALARTLKQDKI
                     GLVTNAPANPGANLLKNSGFEEGIVFPWGTGHYERSDGKFNFGLWWNSKNAHAFMKID
                     TAEKFKGQRSLLIGNKSPTTPHVFTTLSQRIGGLRPNTLIRLSCSLKARDLKPGAGLL
                     TIDAAWAKRFALPSGTYDWRPFTVTVNIGHNDSIDFRIVMQDIGWLWLDDIVVEEVTS
                     AEDVDALQRAESLFDSGNYAQALTIAQEFQSRHPGNEAVLLQYRLLCGRIDMLTGNYG
                     RAFANLRWAIDRGLVRGNIDLGDLYLRLGDFDAAENLFNKMIEQGHFKDDQSTISQLW
                     HRLSQSYLATGKLDQAFKAQQRFSIIARHIGDQHAQATSLHQLAAIQRQQLSWPAARK
                     SLLEAYPIAAQVADRKLLSDIELDLAELAVLLHQNEEAWKYLAAALPLKEAIADRAGW
                     VKGLHLQGQLFVAEGRPDQALTSYGRAVAVLEELAAGAGEISRQAKTAFFQQFAHLYR
                     EYIELLIRRFESTLDPNYHLQAFQIAEQARSRVFSEMLMEARASDTFAATVADPDFKA
                     LLVRDRFATLEIATLERQLQQIKAKADPAEPQALNVRIGKLKLAHQELQDSLRTRYPR
                     YIDLKMPKPMRIQDVQALLDPNEAALSYFVMPRRTALWAITRDTVDLFLLPQSREELI
                     RQSESYRQTFASIANELAHGTEAGLRQVFLKHHAQPAHALYRNLVAPAEASLRGKRHV
                     FLAPDDLLYKVPFETLLTQPFSSNKSADVSVIGIEWQTAPFWVNSHAITYLPSLSVLR
                     SLRQFGKAAVPAQRPLIAFADPDFGSSGAAPQSVNRAATRSAILHALKARRAMTGVTL
                     PPLPETYQEALYVARILGAKPEQDLFVGTRASEYNVKHLRLNLYRNLLFATHGLMAGD
                     FGPGTQPALALSFVGDPENDGLLEMGEILGLDLNADLVVLSACNTASGSGGEDRGEGF
                     AGLTRSFMYAGAKSLLVTQWSVESSSAARLVQSAFAQMATRPRSEALARAKRDMIAAD
                     VRLRISQNTEVSLSHPFFWAPFILVGDQGMRVTGSPNPNAPKNGLSEESVGRF"
     gene            11335..12630
                     /locus_tag="PLB25_02620"
     CDS             11335..12630
                     /locus_tag="PLB25_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOE40519.1"
                     /translation="MSESWKFETKSVHAGYSPDPTTKAVAVPIYQTAAYAFDNAQHGA
                     DLFDLKVAGNIYTRIMNPTQDVLEQRLAALEGGIAALALASGQAAVTYAIQTIAETGD
                     NIVSSSALYGGTYNLLAHTLPLSGITTRFADPRDPASFEPLIDDKTKAVYVESLSNPQ
                     GTVVDIAAIAKIAHRHGVPLIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTSIG
                     GAIVDSGKFPWAEHKARFRRLNEPDQSYHGVIYTEALGPAAYIGRARVVPLRNNGAAI
                     SPFNAFLILQGIETLALRMDRINDNTLAVARFLQLQDKVSWVNYVGLKEHPDHPLAQK
                     YLGGKGSGLLTFGVKSAPGEGRKAGASFLDALQLFTRLVNIGDAKSLATHPASTTHRQ
                     LSPAELIKAGVSEDAVRLSIGIEHIDDLLADLAQALAPV"
     gene            12662..12859
                     /locus_tag="PLB25_02625"
                     /pseudo
     CDS             12662..12859
                     /locus_tag="PLB25_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FCSD flavin-binding domain-containing protein"
     gene            12873..13811
                     /locus_tag="PLB25_02630"
     CDS             12873..13811
                     /locus_tag="PLB25_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HOE40520.1"
                     /translation="MNFQQLRSVRETIRNNFNLTDVAAVLHTSQPGVSRQIRELEEEL
                     GIDIFARTGKRLTGLTPPGDLLLPIVERLLLDCDNLKRVGQEFSSRVDGRLTVAATHS
                     QARYALPHVVRDFRALFPQVTLHLHQGSPKQVAEMLLTGEADIGVATEALALYPQLVT
                     LPCYRWTHSIIVPPGHPLLAHEEPVTLQQLVSYPIITYNQGYTGRSHIDDAFARAGLQ
                     PEVVLTAMDADVIKTYVELGMGVGIVASIALDPERDQQLRILDARHLFEINLTRLAVR
                     RGTWLRGFTYKFIETFAPTLNREVVGQALSESHVPV"
     gene            13918..14160
                     /locus_tag="PLB25_02635"
                     /pseudo
     CDS             13918..14160
                     /locus_tag="PLB25_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006403857.1"
                     /note="catalyzes the formation of acetyl phosphate and
                     sulfite from 2-sulfoacetaldehyde; is active when grown on
                     taurine as a sole carbon source; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
BASE COUNT         2814 a         4061 c         4088 g         3197 t
ORIGIN      
        1 gcctggtcag cgcgcttgat ggcatcctgg ccggactcgt cgggtgcaaa ctgtgcgaca
       61 ccggcgctga aggtgatcag caccttttcg gtgccagcca gaaaaaaacg tttggtcaac
      121 tcgcgctgca gccgagtcat tgcctcaatg cccttttcca gcgtggtgtc aggcagcaat
      181 aagacaaatt cttcgccacc ataacgcgcc agcgtatctt gtggccgcat cacttcgcgg
      241 gcgacacctg cgaggtgcgc cagcgcgaca tcgccggtgg catggcccag cgtgtcgttg
      301 agtttcttga agttgtcaat gtccagcagg gccatgcaca ggggtgtctc gagtcgtttg
      361 accttggata tctcacgtct gaccgcctca tcgagtccgt tgcgattgag tgccccggtc
      421 agtgggtcat ggcgtgcctg cgcactgacc cggtcgagtt cctggtgcag cttggtcagc
      481 tcagcctcgg tggcctcagc ccgcttcttc atgttctgca attcatcgcg cgccgactgg
      541 ctgtcgtggg ccatgtcgcg ggtcgctccg accacgtctt tcagaactgg cgcgatatcg
      601 gtgatggtcc tggcctgttc tatcagacgg gcgctttctt cgagcttgtc gtgaaaactg
      661 ccggtgctct cggacatgga ggccaggcgg tcaacaaagg ttgccagcat ctggcgcatt
      721 tcttcgtgtg cctgcacgct gcgcgacttg gcttcggttt gcttgaagat cacgtctttc
      781 aggcgctgct ccacatcgtc gagtctgcgc agtgtcaacg ggggaacaca aacgttcatc
      841 agcgcgtcca cctgcccctt cagccagccg tcgtcgaggc tcaggtggct gatgttctcg
      901 ataatcaagt gcagcagttt gagcagcaca ttcttgatct cagcctgatc ttccgcggca
      961 aaagacagac ggtgtccata actggcgagc atctgcttga cggcgaccgc atcggcgttg
     1021 ggctgacgca gcgccttgac cagggtttgc gtttgctcca gaaagcgctc atcgtcttgc
     1081 cccaacgccg gcaacgcgta ctcgaccatg cgggcaatct ggctgaaaaa gtcggcagtc
     1141 aaggcgggtg ctgtgagcgg caccgactct gccgggtcga tcatcgtgat gaccggtttg
     1201 gccggttcag ccagtgccag gttgaccgat ttgccgggat ctggggccgg tgggctaaag
     1261 ccaccatagg ccatcaaggc gcttttcacc ccgtcccagt tgagctggtt gatggccgac
     1321 tccagcaaac cgcgttgttt ttgttgccct ggtgttctgg tgggcagcgc cacggcaatg
     1381 tcgcgcaggc gatcggctgg aaaagcgggc tcaatcggct gaccagcaat ttcgctgtac
     1441 aggcgctggt aattgattgg tgtcggtgcc agtttgcgaa cggtcaactg cttgagcgtt
     1501 tcccgtgcaa tctcgaatgg atttttgtct atcatgacat cactctgcta tcaataacat
     1561 aactgctagc gcttattcca taagggttgg aagcctaaaa catcatatat caatgttgcc
     1621 ggcccgcaac gcattggtct cgatgaactc acgccgaggt tcgacctcgt cgcccatcag
     1681 catggtgaac acccggtcgg cttcaatggc atcgtcaatt tgcacccgca gcagggtgcg
     1741 cacagtcggg tccatggtgg tttcccagag ctgctcgggg ttcatttcgc ccaggccttt
     1801 gtagcgttgg cggctggtgg cgtgctcggc ctgaccgatc agccaggtca tggcttcgcg
     1861 gaagtcgcca actttttgct ccttctggcg ctcaccttcg ccacgcatca ctttggcacc
     1921 ctcgccgagc aagcccttaa aggtttttgc cgcttcgttg agtgccgcat agtcagcccc
     1981 atggacaaaa tcctgcgtca gcacgctgct cttgatgttg ccatgcaagc ggcggctgat
     2041 gcgcaaaatc ggcttgtcac tgcggacatc aaattccccg gccacctcgg catcgggcag
     2101 gaaagcctgc atggcggcag ccgagacctc ggcatctgcc accgtgtcaa ggttcagcgc
     2161 gacaccgtcg gcaatggcac gcagcgccga cacatccatg aagttgccca gccgggcgat
     2221 gacggcttgc gccaactggt gtttgcgggc cagctcggtc aagccgtcgc ctgccagcgt
     2281 ggcagcggcc tgtccgccgg taaataccga ggcgttgacc agtgcaatgc gcagcaaaaa
     2341 gttttccaga tcggtctgtc cctgcaaata cagctcttcc ttgcctgcct tgaccttgta
     2401 cagcggcggc tgggcaatat agatgtggcc gcgctcgacc agctctgaca tctggcggta
     2461 gaagaaggtc agcagcaggg tgcggatgtg ggcaccgtcc acgtcggcat cggtcatgat
     2521 gatgatgcgg tggtagcgca gctttgccac gtcaaagtcg tcgttgccgg tgctgccgcc
     2581 agccttgccg atgccggtgc ccagggcggt aatcagcgtc agaatttcgt tgctggtcag
     2641 cagtttttcg tagcgggcct tttccacgtt caatatcttg ccgcgcaagg gcaaaattgc
     2701 ctggaacttg cgatcccgcc cctgcttggc cgagccaccg gctgaatcgc cttcgacgat
     2761 gtagatttca cacagcgccg ggtctttttc ctggcagtcg gccagtttgc caggcaggcc
     2821 catgccgtcg agcacgccct tgcggcgggt caaatcacgt gccttgcggg ccgcttcacg
     2881 ggcgcgggcg gcctcgacaa tcttgccaac aatgatcttg gcatcggccg ggcgctcttg
     2941 caggtagtca aacagcagct ttgacacaat gtcctcgacc ggtccgcgaa cctcggatga
     3001 aaccagcttg tctttggtct ggctgctgaa cttgggctcg ggcaccttga cgctcagcac
     3061 gcaggtcaga ccttcgcgca tgtcgtcacc ggtcacctcg accttggctt ttttggcaat
     3121 ctcggtgtcg tcgatgtatt tgttgatgac ccgcgtcatg gctgcgcgca ggccggtcag
     3181 gtgcgtgcca ccatcacgct gcgggatgtt gttggtaaag cacagcacct gttcgttgta
     3241 gccgctgttc cattgcatcg ccacttccac gccgatgttg gtgttctggt cactctggcg
     3301 gtcgccgagt gcgtgaaaga tgttcgggtt gaggatggtt ttgcccttgt tgatgaagtt
     3361 gacaaagccc ttgacaccac cagcaccgga aaaatcgtcc tctttgccgg tgcgctcgtc
     3421 cttgaggcgt atcttgacgc cgttgttcag aaagctgagt tcacgcagac gcttgctcaa
     3481 aatctcgtaa tggaaatcgg tgttggtctg aaaaatctcc agatcaggca aaaagtgaac
     3541 ttcggtgcca cggtgctcgg tctggccaat cactttcatc ggcgacacct caaccccgtt
     3601 gaccgtctcg atcaggcggt tttgcacaaa acccttgcaa aattccatca catgcacctt
     3661 gccatcacgg cgaatcgtca ggcgcagcat tttggacagc gcattgacgc agctcacgcc
     3721 aacgccgtgc aagccacctg acaccttgta gctgttctgg ttgaacttgc cgccggcgtg
     3781 cagttcggtc agggcgatct cggcggcgct gcgcttgggg tcgtgtttgt cgtccatctt
     3841 gacaccggtc gggatgccac gcccgttatc gaccactgac accgagttgt cggtgtggat
     3901 ggtgaccaag atatcgtcac agtggccggc cagcgactcg tcgatcgagt tgtccaccac
     3961 ctcaaatacc agatggtgca gcccggtgcc gtccgaggtg tcaccaatgt acatgccagg
     4021 gcgcttgcgc accgcttcga gcccttccag aatctggatc gagccctcgc cgtaggcttc
     4081 agaagcgcca gcctgatggg catcgatggt cggctgaaag ttggagctgt cagcgttgtc
     4141 atcgcctgtg cgaacggtat cttcgatggg caagacaggc ttgttttctt cggtcatgag
     4201 gcactcttga tcaggttggg gcagggcacc gagtcagttg ccgcgcgaaa tttcatggat
     4261 tttcggaact ctggaaacat caaacccgct agaaacagag cgttcatcgc gggtttgttt
     4321 tgtcaacttg caaggtacgc tggctaaatt cgcatcggca tgacgacata tttgaaatgg
     4381 tcgatttcag ggatcgtcaa cagcgccgaa ctgttgccgt ccgagatctc gatcttgatc
     4441 atctcctgcg acatattcga cagtgcatcg atcaggtaag tgacgttaaa gccaatttcg
     4501 atgctgtcgc cgctgtagtc aatgtcgagt tcatccaccg cttcttcctg ctcggcgttg
     4561 ttggccgcca cccgcagtgt gccggggtcg atgttcagcc gcaccccctt gaacttgtcg
     4621 ctggtcaaaa tggcggtgcg ctgcagggtg gacaggagcg cggcgcggcc caggatgatg
     4681 ctgttttgat ggttgcgcgg gatgacacgg ttgtaatcgg gaaacttgcc ctcgaccagc
     4741 ttggagacaa actccatgcc gccaaagctg aacttggcct ggttgttggc aaactgcatg
     4801 tcaatcgcgc cttcagcatc ggacagcagg cgctgcaact caagcactgt cttgcgcggc
     4861 aaaatcacct cctgtttggg cacttcaacc tctagcgtgc tcgatgcaaa cgccagccgg
     4921 tgaccatcgg tggcgaccaa gctgagctgc gggccttctg ccacaaacaa gatgccattg
     4981 aggtaatagc gaatgtcatg cacggccatg gcaaacgaca cctggctgag caagcctttc
     5041 agggtttttt gtggcacgct gaacaccggg ccaaaattgg ccgattcctg caccagcgga
     5101 aagtcctcgg ccggcatggt ctgcagggtg aatctgcttt tgccgccctt gaggatcagt
     5161 ttggccgcgc tggattcgag cgacaccacc tggtcagcgg gcatgctgcg caaaatgtca
     5221 atgagcttgc gggcaccaac cgtggtgttg aagtcaccgt catcgccacc aagttcagca
     5281 gaggtgcgaa tctggatttc caggtcgctg gtggtaaaca gcagtttttg accggtcttg
     5341 cggattaaca cgttggccag aatcggcagg gtgtggcgcc gctccacaat acctgagacg
     5401 gcttgcagta ctgccagcac tttgtcctgg gtgtctttta aaacaatcat gtcttcctct
     5461 tgtggtcaat cgtcaaatgg gctgttggcg cggtactctt gccagttatc gcagactgca
     5521 aaagcgctat tttcccactc atttggctgg tttggctggt ttggcgctag cctttgagcg
     5581 tttgctccag cacatggagc tgctggttga gttcggtcat ttgctggcgc tccccgccaa
     5641 tcttgcgcac cgcatgcagc accgtggtgt ggtcgcgccc gccaaacagc tcgccaattt
     5701 ccggcaggct tttttgggtc agttccttgg ccaggtacat ggcaatctgg cgcgggcggg
     5761 caatgctggc cgggcgcttc ttggagtaca tgtcggcgat cttgatcttg tagaagtcgg
     5821 ccaccgtctt ctggatgttt tccaccgaga tctgccgatt ctggattgac agcaggtcac
     5881 gcagggcttc gcgggccagc atgatggaaa tttccttctg gttgaaacgc gagtacgcca
     5941 ggattttgcg caacgcacct tcaagctcgc gcacatttga gcgcacattc ttggccacga
     6001 aaaaggccac ttcctctggc atcacaatgc cctcgacgtg cgccttgttg atcagaatgg
     6061 cgacccgcat ttcaagttcg ggcggctcaa tggccaccgt caaacctgaa tcaaaacgcg
     6121 aaaccagtcg ctcatggatg tccgccaaac ccttcgggta ggtgtcgctg gtcatgacaa
     6181 tgtgcgactt gcgcgccagc agggcttcaa aagcgttgaa gaactcctcc tgcgtgcgct
     6241 ccttgttggc gaaaaattgc acatcgtcga tcagcagcaa gtccagcgaa tggtagcgtt
     6301 ccttgaattc atcaaaggcc ttgcgctggt aggccttgac cacatcggtg acaaactgct
     6361 cggcatggat gtaaagcact ttggcgttgg gccgttcgct gatcagttgg ttgccgaccg
     6421 catgcatcag gtgcgtcttg cccaagccga ccccgccgta aatgaacaaa gggttgtaaa
     6481 gatgcccagg catgcccgct acgtgcatcg ctgcggcgcg cgccatccgg ttggcgctac
     6541 cctctaccag ggcatcaaag gtcagcgcgg tgttgagccg acttttcaat ggctcgttgg
     6601 caggtgtggt gtcggctgat gtcgtcacat cagcctggct ggcaccagac tgatgaagca
     6661 gcggtcttct gccggacgct tcgcgcgggg caatcgccag ctcaatctga accggctggc
     6721 cagtcaggcg ctccatcaac agcgccaggc gcccactgta ctgggccctg atccagtcca
     6781 gcttgaagcg gttggcgaca aacagcgtga ctctggacag gtcatcggcg acttgtgctg
     6841 tcagcggctt gatccaggtc tggaactgtt gttcgggcag ctcctgcgac agttggtcaa
     6901 tacaggcttg ccagaaatcc gggttgggaa tggcttgttc agcctcgggt agtgggggtt
     6961 ctccctcaat catcatgcgg tcagtctttg tggatagttg ctgatttatg ctagcacaga
     7021 tccgatttat caaatgctta tccacattgc cggtggcaac cggggcggct gcgcaaaacc
     7081 gtgacctgtt ccctgcgatt ccatttataa atcatcgatg ttgttgttat ctcgccttgc
     7141 cttgcgtttg caatggctgc ggcagggtgt ctatcccagg ctgattgcaa caacatactg
     7201 gttgatgtaa ggcgccgagc gcctcatttt tttaaagacg acggactccg atgcagatgc
     7261 aggatcaatt cggctgaaca cacgcagttt ctgctcgggt agcctgagtt gcgtgcatcc
     7321 ccgtgcgtca gcatctgcta aaggcgcgac tgcaatcatc aaaatctacc cacggactcc
     7381 tcagacaggc cgtttttcgg tgcattcggg tttggcgagc cagtaacccg catgccttga
     7441 tctccaacca gtatgaaggg tgcccagaag aacgggtgag acagtgaaac ttcggtattt
     7501 tgactgatcc gcagccggac atctgcggca atcatgtcgc gcttggctcg ggccaacgct
     7561 tcgctgcgtg ggcgagtggc catttgcgca aaggccgatt gcaccagtct ggcggcggaa
     7621 ctgctctcga cgctccattg agtgaccagc agtgatttgg caccggcgta cataaaactg
     7681 cgggtcaaac cggcgaagcc ttcgcctcga tcttcgccac cactgccgct ggccgtattg
     7741 cacgctgaca gaacaaccag atccgcgttc aaatccaggc cgaggatttc acccatttcc
     7801 aacagtccat cgttctcggg atcgccgaca aaactaagag ccagagccgg ttgcgttcca
     7861 gggccaaagt caccggccat cagcccgtgg gtggcgaaca gcaggttgcg gtaaagattc
     7921 agtcgcaagt gtttgacgtt gtattcgctg gccctggtgc cgacgaacaa gtcttgttcg
     7981 ggcttcgctc cgagaatccg tgcgacataa agtgcctcct ggtaggtctc tggcaagggt
     8041 ggcaacgtga ccccggtcat ggcacgccgt gctttcaagg catgcaggat ggctgaccgg
     8101 gttgcggcac ggttgacgct ttggggtgct gcgcccgaag atccaaaatc cggatcggca
     8161 aacgcgatca gcggacgctg tgccgggacc gcagccttac cgaactgccg caagctgcgc
     8221 aggaccgaga gcgacggcaa ataggtgatg gcgtggctgt tgacccagaa tggcgcggtt
     8281 tgccattcaa taccaatgac tgaaacgtcg gcgcttttgt tgctgctgaa gggttgcgtc
     8341 aacaaggtct caaatggcac tttgtaaagc aggtcatccg gtgcaagaaa aacgtggcgc
     8401 ttgccgcgca agcttgcttc ggcgggtgcg accaagttcc gatacaaggc atgggccggt
     8461 tgggcgtgat gtttgaggaa gacttggcgc aggcctgcct cagtgccgtg cgccaactcg
     8521 ttggcaatac tggcgaaagt ctggcgataa gactcgcttt gccggatcaa ttcctcgcgg
     8581 ctttgcggca acaggaacag gtcgactgtg tcgcgcgtaa tcgcccagag tgcggtgcgc
     8641 cggggcatga cgaagtagct cagcgctgct tcgttcgggt caagcagcgc ctggacatcc
     8701 tggatgcgca tcggcttggg catcttcaag tcgatatagc gcgggtagcg agtgcgcaag
     8761 ctgtcttgca gttcctgatg cgccagcttc agcttgccaa tgcgaacgtt gagtgcctgc
     8821 ggttcagccg gatcagcctt ggccttgatt tgctgcagtt gcctttcgag ggttgcaatc
     8881 tcgagtgtgg cgaagcgatc cctcaccagc agtgccttga aatcaggatc tgcaactgtt
     8941 gcagcaaaag tatcgctggc acgtgcttcc atcagcattt ctgaaaatac ccgcgaacgt
     9001 gcctgctcgg cgatttgaaa ggcttgcagg tggtagttgg ggtcaagggt cgactcgaag
     9061 cgacgaatca gcaattcgat gtattcccgg tagaggtgcg caaactgctg gaagaaagca
     9121 gtcttggcct gtcgcgatat ttcgccggca cctgcggcca gttcttccag cactgccaca
     9181 gcgcggccat agctggtcag tgcctggtca ggacggccct ctgcgacgaa caactgtcct
     9241 tgcagatgca ggcccttgac ccagccggcc cgatcggcga tggcctcctt caacggcaat
     9301 gctgctgcca ggtatttcca ggcttcttcg ttctggtgca gaaggacagc aagctcggcc
     9361 aaatccagct caatgtcgga aagcagtttg cggtccgcaa cttgtgcggc gatcgggtag
     9421 gcctcaagga gggacttgcg agcagcaggc cagctcagtt gctgacgctg tatagcggcc
     9481 aattgatgaa gcgatgtcgc ctgcgcatgc tggtcgccga tatgcctggc aatgatgctg
     9541 aagcgctgtt gcgccttgaa tgcctgatcg agcttgccgg tcgccagata ggactggctc
     9601 agccgatgcc agagttggct gatggttgac tggtcgtcct tgaagtggcc ctgctcgatc
     9661 attttgttga acaggttttc ggcggcatcg aaatcaccca agcgcagata caggtcaccg
     9721 agatcgatat tgccgcgcac cagtcctctg tcgatggccc agcgcaggtt cgcgaaggct
     9781 cggccatagt tacccgtcag catatcgatg cggccgcaga gcaggcggta ttggagaagc
     9841 actgcttcgt tgccagggtg gcggctctgg aattcctgag caatggtcag cgcctgggca
     9901 tagttgcccg agtcaaacag gctttcggcg cgttgcagcg cgtcgacatc ttcagccgat
     9961 gtcacctcct cgaccacgat gtcgtcgagc caaagccagc caatgtcctg catgacgatg
    10021 cgaaagtcga tgctgtcgtt gtggccgata ttgacggtaa ccgtgaatgg tcgccaatca
    10081 taggtcccgg agggcaaggc aaagcgtttc gcccaagcgg catcgattgt cagcaaaccg
    10141 gcacctggtt tcaaatctct ggctttcaga gagcaactca ggcggatcag cgtgtttggc
    10201 ctgagtccgc caatgcgctg tgagagtgtt gtgaacacat gcggtgtagt tggcgacttg
    10261 ttgccaatca gcaaagagcg ctgtcccttg aacttttcag ccgtatcgat cttcatgaac
    10321 gcatgggcgt tcttgctgtt ccaccacagg ccaaagttga acttgccatc tgatcgctcg
    10381 taatgcccgg tgccccacgg gaaaacaatg ccttcctcaa agccgctgtt tttcagcaaa
    10441 ttcgcgccgg ggttggcggg agcgttagtg acaagcccaa ttttgtcctg cttaagggtt
    10501 ctagccaggg cgaagctatg tccaaacgcc gccatctcgc cttgtgccat gtgtgccgct
    10561 gcgatgttgt tgatgacttc tgcattcagt gggctgcttg cgtcaattct ggatgaggct
    10621 tgttgatagt gtttcagcgc cagcgcgaac tccctgtcgt tgaaaaactg atcggcttgg
    10681 cggatggtct gctcgacctc ctgtgcacca gcagcagcaa aggcaaacag gcagaccaac
    10741 ccttttgcaa gtgcgatcag gtagcgtaaa accggtctat ccatttagtc actactgcaa
    10801 cgggtaacca actcggcatg attcaaactt ggctaacact ttagcgtatg cagcttggct
    10861 cgtcgatatc cttttgatct tcacgctgga taactggcgc ggcaaccaag cctttcaccg
    10921 cagcaccacg cagatctcgc ccgacacgtc aaaagccgca attgggtagc cctgcccagc
    10981 accccaaaat ggcgctgaac ctttcaatag catcttgcct attgtgcagt gatgcaccaa
    11041 caaaaccaga tcaagcggac ccgcgcgcag cccgaattga tcaagatcgt tcgcagccaa
    11101 gttgccaaca gtgttcactg aaaacgcctc tttgcgtatc aacctgggcc aatatgcgtt
    11161 tttgtcatta ggaaattacc tttaagtcgt ttgcgttttc agagatggcg catagagttg
    11221 cgtcagccac tgtgatcgat ttcgccgcta gcgctttata caaaagcgtc agcagctatt
    11281 atttttgcag cattggtgag tttgtcgcga tcactgcaac ccaaggagct ttctatgtcc
    11341 gaatcctgga aatttgaaac caaatctgtt cacgctggct actcgccgga cccgaccacc
    11401 aaagcagtcg cagttccaat ctaccaaaca gccgcctacg cctttgacaa cgcacagcat
    11461 ggtgcagatt tgtttgacct gaaagtggcg ggcaacatct acacccgcat catgaacccg
    11521 acgcaagatg tgctggaaca acggctggcg gcactcgaag gcggcattgc tgcgctggca
    11581 ctggcatccg gacaggcagc ggtgacctat gcgatccaga ccatcgccga gaccggcgac
    11641 aacatcgtgt cgtcgagtgc gctgtacggc ggcacctaca acctgcttgc ccacacgctg
    11701 cccctgtcgg gcatcaccac ccgctttgcc gacccgcgcg accccgccag ttttgagccg
    11761 ctgattgatg acaagaccaa agcggtttat gtcgagtccc tgagcaatcc gcaaggcacg
    11821 gtggttgaca ttgccgcaat cgccaaaatt gcccaccgcc acggcgtgcc gctgattgtc
    11881 gataacacgg tggccacgcc ttacctgctg cgcccgttcg agcacggtgc cgatatcgtg
    11941 gtgcattccc tgaccaaata cctgggcggg catggcacca gcattggtgg tgccatcgtc
    12001 gattccggca agttcccctg ggccgagcat aaggctcgct tcaggcgtct caacgagccc
    12061 gaccagagct accacggtgt gatttatacc gaagcccttg gcccggcagc ctacattggc
    12121 cgcgcccgcg tggtgccact gcgcaacaac ggtgccgcca tttcgccgtt caacgctttc
    12181 ctgattctgc agggcattga aaccctggcg ctgcgcatgg accgcatcaa tgacaacacg
    12241 ctggcagtgg ccaggttcct gcaactgcaa gacaaggtgt cgtgggtcaa ctatgtgggt
    12301 ttgaaggagc acccggatca tccactggca caaaagtacc tgggcggcaa aggctcgggc
    12361 ctgctgacct ttggtgtcaa gtcggcacct ggcgaaggtc gcaaggccgg tgcgtccttt
    12421 ctggatgcgc tgcaactgtt cacccgcctg gtcaatattg gggatgccaa gtcgctcgcc
    12481 acccacccgg catcaaccac gcaccgccag ttgtcacccg cagagctgat caaggcgggg
    12541 gtctctgaag atgcggtgcg cctgtcgatt ggcatcgagc acattgacga tttgctggcc
    12601 gacctggcgc aggcgctggc gccagtctga gcctgcacct gaaaggctga aaggagaaca
    12661 gatttttgcc aatacctgct acagctttgt ctctgacacc gaggtggtgc atgtggcttc
    12721 ggtgcacaag tacgaccggg agaagaaaac cctggtggca gtcaatggcg ccgggggact
    12781 ctcgccacaa gccaacgtgc tggagggtca atacgccaag gcttgggcgc gcaacatctg
    12841 ggctgacacc ctgctatgaa accagtacac aaatgaactt tcaacaactc cgctcggttc
    12901 gggaaaccat tcgcaacaac ttcaacctga ccgatgtggc agccgttctt cacacatcgc
    12961 agccgggcgt gagccgccag atccgggagc tggaagaaga gttgggcatc gacatatttg
    13021 cccgcaccgg caagcgcttg accgggctga ccccgcccgg tgacttgttg cttccgattg
    13081 tggagcggct gctgcttgac tgtgacaacc tcaaacgtgt cgggcaggaa ttcagttccc
    13141 gcgttgatgg tcgccttacg gtggccgcaa cccactcgca ggcacgttat gcgctgccac
    13201 atgtggtgcg cgactttcga gcgctgtttc ctcaggtcac actgcatctg catcagggct
    13261 caccgaaaca ggtggctgaa atgctgttga cgggtgaggc cgatatcggc gtggccaccg
    13321 aggcgctggc gctctatcct cagcttgtga ccttgccatg ctaccgatgg acccatagca
    13381 tcatcgtgcc gccgggtcac ccgctgctgg cgcatgagga acctgtcacc ttgcagcaac
    13441 ttgtcagcta tccgatcatt acctacaacc agggctatac cggcagatca cacattgacg
    13501 atgcatttgc ccgggccgga ctgcagccag aggtggtgct gactgccatg gatgccgatg
    13561 tgatcaagac ctatgtcgaa ttgggcatgg gtgtgggcat cgtcgcctcc atcgcgctcg
    13621 atcctgaacg cgatcaacaa ctgcgtatcc tggatgccag gcacctgttc gagatcaatc
    13681 tgacccggtt agccgtgcgg cgcggtacct ggctgcgtgg ttttacctac aagttcattg
    13741 agacgtttgc cccaacgctc aatcgcgagg ttgtcgggca ggccttgtct gagtctcatg
    13801 tgccggtata aatgtcgcca tcgacggaca ttgggcgcaa gatgagccgg atcaatcgcc
    13861 ttcgctggaa ctttctgggc ttgatgggtg cattgatggg tgcattgatg gcttcaagac
    13921 agatgctttg tcgcaggcga gctcgacaac cccagcttgg ccggcattgc cggggccgag
    13981 ggcatcgtcg ttgaccggct cgaagaggtc ggcccggcgc tgcaaaaagc catagcggcg
    14041 cagatgaacc acggcaaaac gaccgtcatc gagatcatgg tcacccgtga gctcggcgac
    14101 ccgttccgcc gcgatgcgct gtcgcgaccg gtgcggctac tggacaagta ccaggactat
//
LOCUS       DAOSLL010000035        14116 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4412_length_14116_cov_5.172036, whole genome shotgun sequence.
ACCESSION   DAOSLL010000035 DAOSLL010000000
VERSION     DAOSLL010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 14116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14116
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4412_length_14116_cov_5.172036"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5668)
                     /locus_tag="PLB25_02640"
     CDS             complement(<1..5668)
                     /locus_tag="PLB25_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HOE40521.1"
                     /translation="MNLRNIVLAGGLLALSALAQAFQVVSVSPQGEVARITQLVVKFD
                     ESAVNFGDPKAPAPLDLQCSNPQATQGTGRWLNDRQWVYDFEADLPPGVACTIKLKSG
                     IKSVKGAVLTGSGSYKFNSGGPFVQEIWPGTDTPIDEDQFFVLQLSGPATLASVLANT
                     WCAVEGLGERVAVRQVDGKDRAALLKFHQLETQAAKTPLSFVTLACNRRLTPSAKVQV
                     VYGKGVSTPGTASNPQGIANSVEKRFGFQVRVPFAATFACERENAQAACLPIRPMRLH
                     FTAPVARKLLEGIKLASDKQTYSPSFEQDEEGDADNVVSRVSFKGLFAEQTSFKLTLP
                     KEFKDASGRPLVNADSFPLKVSTGAMPPLAKFAAASFGIVERFAEPDGVAMLPITLRN
                     VEADLKIKALSPANGKVSDLKIEADTDIIAWFRKVKRFDDYSVPRSVAAREVKGALPQ
                     VLPDEDKTEVQARMVSLLQGRAGVKTLDLPKPASNDPRPFEVVGIPLTPGFHVLELAS
                     QKLGSSLLDERHGAGRTMYVRTSALVTNLGVHFKLGRENALAWVTTLDKGQPVANAQV
                     RVSDCQGQQIASASTNAQGIAYLTGISPKAPPCRGEDGYSNAYFVSARAQLAGAADMA
                     FTWSDWNRGIEPWRFNLPTSQEARPDERAHTIFDRTLLRVGETVSMKHLLRSETAQGF
                     GLTQAQPATVQITHIGSGQRYSQPLVWRKTATGGLSAESSFAIPPAAKLGNYEVRLQG
                     SDNNRSFNTGEFRVEEFRLPVLEGNIAPTDKKALVNLKALPLSVQVNYVAGGGASSLP
                     VRVSAMVRSKYIHFSDFDAFSFSPPRTDQASSTEHAEEESASQDNRVVADKLALTLDK
                     NGAGKLTIDKLPASREPQDLVLEASYADPNGEVQTIRSTQTLWPSGVVAGIKTEGWVS
                     SSQKIKFQALALDTSGKPLADVALEVKATARITTSSRKRMVGGFYSYDNKTSVKELGS
                     VCSGKSDARGLLMCETSLNQAGEVELVVSAKDGAGNSSQAASSVFVTKQGELWFGGED
                     HDRIDLIPEKKSYQPGETARFQVRMPFRFATALVAVEREGILQTEVVQLNGQDPTVQV
                     KVKDGWGPNVYVSVLALRGRLREVPWYSLFTWGYKSPREWWTSFWYEGREYVAPTALV
                     DLSKPAFRLGTAEIRVGTQAHQLAVSVKADKESYPVRSQAQVTINVKLPSGQPAAHAE
                     VALAAVDQALLELKPNTSWNLLDAMLQRRSWGVETATAQMEIIGRRHYGRKAVPAGGG
                     GGRGGTRELLDTLLLWNPNIKLDANGSATVMVPLNDALTTFKIVAIADAATGLFGTGS
                     TSIRATQDLQIISGLPPLVREDDQYRAQITLRNTTKQAMKVLVTPRATLLELPPQTVD
                     IPAGDAREVAWSVKAPAQLALSRFEAILWEIEAKDTLGGARDALKASQRIIPAVPLTV
                     QQATLVQIDGPFSLPVSPPADAIAGRGGLKLSLQPRLAEGLPGVKDWFANYPFVCLEQ
                     KTSKAVGLRDGKLWRIVAAQIPSYLDGDGLANYFPPRDGQANQGSDTLTAYLLAASHE
                     AASIDPAFALSDEVRAPMEKALVAFVEGRLQRNFWSPRKDLDMRKLAAIEALSRYGKA
                     QARMLESITLAPNQWPTHAVIDWLNLLKRLPDLADRDKRLAEASQILRGRISYQGTKM
                     MFSTERDDYWWWLMQNGDVNTARLMLAVLDDPAWKDDMGRLANGFMARQQNGAWHTTT
                     ANLWGGLALEKFSNKFESVAVAGSTKASLGASSAAVDWSRVERIKTTDASGAAHQSTW
                     FGAPAAPGNLRNNSMFLPWGKDAGKDTLSVTHQGPGKPWLTLQSVSAIQLKAPFAAGY
                     QIKKTITPVEQANPGLPAGSYSRGDVLRITLEVNASADMTWVVITDP"
     gene            complement(5714..6241)
                     /locus_tag="PLB25_02645"
     CDS             complement(5714..6241)
                     /locus_tag="PLB25_02645"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HOE40522.1"
                     /translation="MTDTTSSPNPAPTPPCHVTRWRVATIICLILLAIAAATGVSMME
                     QFKAQLIHLQTKLKSVPQVKYVAVLLDDKHLPAQLITFDPLDGYLLIQRLNDVKEGQE
                     DSMQLWALDEQDQPLSLGVLTTKIKTAQIPVTDKILSQARGLAISVEKKGGVEAGSPP
                     RLPYLFTGVLIRKTL"
     gene            complement(6369..7820)
                     /gene="argH"
                     /locus_tag="PLB25_02650"
     CDS             complement(6369..7820)
                     /gene="argH"
                     /locus_tag="PLB25_02650"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HOE40523.1"
                     /translation="MSSNQFDQKSQAWSALFSEPMSDLVKRYTSSVFFDKRLWQADIS
                     GSLAHAEMLAAQGIISNQDHADIERGMAQISSEIESGSFEWQLDLEDVHLNIEARLTQ
                     LVGDAGKRLHTGRSRNDQVATDVRLWLRDETDLIGALLTDLQKALLAVAEQHVDVIMP
                     GFTHLQVAQPISFAHHMLAYVEMFARDAERLQDVRRRVNRLPLGAAALAGTSYSLDRE
                     RVAMSLGMVDQQGLPCVCQNSLDAVSDRDFAIEFSAAAALCMVHISRLSEELILWMSQ
                     NFGFIRIADRFTTGSSIMPQKKNPDVPELARGKTGRVVGHLMGLITLMKGQPLAYNKD
                     NQEDKEPLFDTVDTLKDTLRIFAEMVGGQINPASGIKEGGIAVNAQAMERAALRGYAT
                     ATDLADYLVKKGLPFRDAHETVAHAVKAAISHQVDLSELPLAVLKEFNPGIEKDVFDV
                     LSLRGSLNARDVLGGTAPKQVRAQIARHQARLG"
     gene            7962..9017
                     /locus_tag="PLB25_02655"
     CDS             7962..9017
                     /locus_tag="PLB25_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOE40524.1"
                     /translation="MQDSQILSVFQEPATLASDIGQPFMLHDALVFDACHVGVVLRAV
                     LFVEAVVGVVTMFGASSAVQWLGQMALVTGAALPATLLWLVAACSLKRLLARLSPTAQ
                     KLAGVALGAVAGAYGCGMLVVIGLLDSAPWWASACSGAMLSAALVVALVLRAKGKTPA
                     ATAARLAELQARIRPHFLFNTLNSAIALVRAEPARAERLLEDLSDLFRYALAEQSASV
                     SLADEIALAQRYLAIEQVRFGDRIQVQWTLDASARQARLPPLLLQPLIENAVKHGVEP
                     SAGVTHIKVSTERRGATVVIKVSNSMSQPSTVQAPGHGLALNNVRERLSLLHDVQGSF
                     RAGYKNGIFQVRIEVPL"
     gene            9041..9787
                     /locus_tag="PLB25_02660"
     CDS             9041..9787
                     /locus_tag="PLB25_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOE40525.1"
                     /translation="MALRVLIVDDEALARSRLCRLLADCTSPGALVVGEVANAADAMQ
                     FVTHHAVDAVLADIHMPGADGLTLAQNLRALPNPPIVVFVTAYAEHAVQAFELEAQDY
                     LTKPVRLERLQVALQKVERARQAAKGPEPDLTEEVLVIQERGRTERVPLSAVLYLKAE
                     LKYITVRTAGHSYILEGALSDLEARYASRLIRIHRNTLVARRAVRSLQRHHDAQEGEG
                     WAVRLDGLAEPLYVSRRQLVAVRALVAARD"
     gene            complement(9844..10770)
                     /locus_tag="PLB25_02665"
     CDS             complement(9844..10770)
                     /locus_tag="PLB25_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOE40526.1"
                     /translation="MSTSKATLPHRKPPARNGVGPSCVSLTGNHWPTLLDFLLARFPN
                     VTGQTWLERIRRGDVFDATGVAIDPLRASQPPGPGQTLLYYFREVPNEVPIPFDEVLL
                     YQDDHLLVVDKPHFLPVVPSGDYLAETLLVRLKRKLGLSDLVPIHRIDRDTAGLVLFS
                     KQPASRAAWCAMFSAHQVQKTYEAIAPWRAGLSWPVTRHSRIKEAGHFMLQHEVDGVP
                     NATTQIDVLEVRGDLARYQLRPVTGQRHQLRVHMLALGLPILNDGLYPVLTPKGQSDH
                     TRPLQLLAKTLAFTDPITGGARHFVSARALAF"
     gene            complement(10880..11656)
                     /gene="trmB"
                     /locus_tag="PLB25_02670"
     CDS             complement(10880..11656)
                     /gene="trmB"
                     /locus_tag="PLB25_02670"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HOE40527.1"
                     /translation="MPTDPAQPGKPRQMPAGVDYPREIRSFVRRAGRTTNGQSRAFEE
                     LGPRFLLPYQSDAIDLEAAYADPIRAAGQNDSKYRPLVLEIGFGMGEATAQIAALLPE
                     TRFLCCEVHQSGVGALLKRIGEQGLRNIRICAHDAVAVIDHMLTLQSLDGIHIFFPDP
                     WHKARHNKRRLIQPPLVAKLAARLKVGGYIHCATDWQAYAEQMLAVLSAEPLLKNSAL
                     AAHPQLQGYAPKPHYRPLTKFENRGLKLGHGVWDVVFERV"
     gene            11823..12008
                     /locus_tag="PLB25_02675"
     CDS             11823..12008
                     /locus_tag="PLB25_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40528.1"
                     /translation="MMASARLPGKIFTGFVGLLKEHVGKKLTLIIDNASIHKAKATSH
                     LMDVLEKQAVTLYFLPP"
     gene            12100..12522
                     /locus_tag="PLB25_02680"
     CDS             12100..12522
                     /locus_tag="PLB25_02680"
                     /inference="COORDINATES: protein motif:HMM:NF022970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HOE40529.1"
                     /translation="MDELDKQDKRIAAILNEDEPPEVTYETLRKYVTHLRANIVLPCS
                     LTGIEDFDWEERYVIGGGDKKEYEKLKKTRPSYTDVFSLLSFSDDVDEMYGIFVKVKR
                     ASDKKQFTLPLADLEVIDINSSNFQLVNDFSVWFVNNS"
     gene            12739..13422
                     /locus_tag="PLB25_02685"
     CDS             12739..13422
                     /locus_tag="PLB25_02685"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HOE40530.1"
                     /translation="MCVAQTQVIALVAANAVAIRRFDRVDGQRIHSVSAGTAIRAATV
                     AGTEPEMGYPQLARILRRIGLTQGDAHLTDAQELFRRMVFNILIDNADDHEKNHALLV
                     VAPAANGRLRLAPAYDVLPTNSGQGYQEFICGAFGRESTLANAMSQCDAFGLQPAQAA
                     AQVVKVIGVVNTWRAHFDAIRVSKSDLEGLAERLDGDELLGHRQRLDAGQYQVAAPKR
                     RPTSPFRRT"
     gene            complement(13784..>14116)
                     /locus_tag="PLB25_02690"
     CDS             complement(13784..>14116)
                     /locus_tag="PLB25_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655568.1"
                     /note="DppABCDF is involved in the transport of
                     dipeptides; also binds heme and mediates chemotaxis to
                     dipeptides; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE40531.1"
                     /translation="QMGMLGWTGDNGDPDNFLNTLLGCSAAKNNGSNVAKFCYQPFED
                     LVQKAKVVSSPAERTKLYEQAQVIFKQQAPWFTIAHAVQLKPVRKEVIDFKLSPFGRH
                     SFYGVDMK"
BASE COUNT         2582 a         4145 c         4420 g         2969 t
ORIGIN      
        1 ccgggtcggt gatgacgacc caggtcatgt cggcgctggc gttgacctcc agcgtgatgc
       61 gcagcacatc accccggctg tagctgccag caggcaggcc cgggttggcc tgctccaccg
      121 gcgtgatggt cttcttgatc tggtacccgg cagcaaacgg cgctttcaac tggatggctg
      181 acaccgactg cagggtcagc cagggtttgc cggggccctg gtgcgtcacg ctcaaggtgt
      241 ccttgcccgc atccttgccc cacggcaaaa acatgctgtt gttgcgcagg ttgcccggcg
      301 ctgccggggc accaaaccag gtgctctggt gcgctgcgcc gctggcatcg gtggtcttga
      361 tgcgctccac gcggctccag tcaaccgctg cgctcgatgc gcccaggctg gctttggtgc
      421 tgccggccac tgccacagat tcgaatttgt tggagaattt ttccagcgcc agaccgcccc
      481 acaggttggc ggtggtggtg tgccaggcac cgttttgctg gcgcgccatg aagccgttgg
      541 ccagccggcc catgtcgtct ttccaggccg ggtcgtccag caccgccagc atcaggcgcg
      601 cggtgttgac atcgccgttt tgcatcagcc accaccagta gtcgtcacgc tcggtgctga
      661 acatcatttt ggtgccctgg taactgatgc gtccgcgcaa gatctggctg gcttctgcca
      721 gccgcttgtc gcgatcagcc aggtcgggca gacgtttgag gaggttcagc cagtcgatca
      781 ccgcatgggt tggccactgg ttgggtgcca gggtgatcga ttccagcatg cgagcctgcg
      841 ccttgccata gcgcgacagg gcttcgatgg cggcgagttt gcgcatgtcg aggtctttgc
      901 gcgggctcca gaaattgcgc tgcaggcggc cttcgacaaa cgccaccagg gctttttcca
      961 tcggcgcgcg cacctcgtcg ctcagggcaa aggccgggtc gatgctggcg gcttcgtggc
     1021 tggcggccag caggtaagcg gtgagcgtgt cgctgccctg gttggcctgg ccgtcgcgcg
     1081 gcgggaaata gttggccagt ccgtcgccat caaggtagct gggaatctgc gccgccacaa
     1141 tgcgccacaa cttgccgtcg cgcaggccga ccgccttgct ggtcttttgc tccagacaga
     1201 caaacgggta gttggcaaac cagtccttga cccccggcag gccctcggcc agcctgggtt
     1261 gcagcgacaa tttgaggcca ccgcgcccgg caattgcatc cgctggcggg ctcaccggca
     1321 gactgaacgg cccgtcaatt tgcaccagcg tggcttgctg caccgtcagt ggcactgccg
     1381 ggatgatgcg ctggctggcc ttgagcgcat cgcgagcgcc gccgagcgtg tctttggcct
     1441 cgatttccca caggatggct tcaaaacggc tcaaggccag ttgcgccggg gccttgacgc
     1501 tccaggcgac ttcgcgcgcg tcaccggcgg gaatatcgac cgtttgcggc ggcaactcca
     1561 gcagcgtggc acgcggtgtc accagcacct tcatcgcctg tttggtggtg ttgcgcagcg
     1621 tgatctgggc gcggtactgg tcgtcttcgc gcaccagtgg cggcaggccg ctgatgattt
     1681 gcaggtcttg cgtggcgcgg atgctggtgc tgccggtgcc aaacaggccg gtggcggcat
     1741 cggcgatggc gacaatcttg aaggtggtca gcgcgtcgtt gagcggcacc atcacggtgg
     1801 cgctgccgtt ggcatcgagc ttgatgttcg ggttccacaa cagcagggtg tcaagcagtt
     1861 cacgggtgcc accacggcca ccaccaccac cggctggcac cgctttgcgg ccatagtggc
     1921 gtcggccaat gatttccatc tgcgcagtgg cggtttcaac gccccaggaa cggcgttgca
     1981 gcatggcatc gagcaggttc caactggtgt tgggtttgag ttcgagcagc gcctggtcaa
     2041 ctgcggccag cgccacttcg gcatgggcag ccggttggcc gctgggcagc ttgacgttga
     2101 tggtgacctg ggcctggctg cgcaccggat agctttcttt gtcggccttg acactcaccg
     2161 ccagttggtg cgcctgcgtg ccgacccgga tttcggcggt acccaggcga aacgccggtt
     2221 tgctcagatc gaccagcgcg gttggggcga cgtattcgcg cccctcgtac caaaatgagg
     2281 tccaccattc gcgtggcgac ttgtagcccc aggtaaacag gctgtaccaa ggcacctcgc
     2341 gcagccggcc gcgcagcgcc agcacactca cgtacacgtt aggtccccag ccgtccttga
     2401 cctttacctg gaccgtcggg tcttgcccgt tgagctgcac cacctcggtt tgcagaatgc
     2461 cttcgcgctc gaccgccacc agcgcggtgg caaagcgaaa cggcatgcgc acctgaaaac
     2521 gcgcggtttc acccggctga tagctctttt tctccgggat caggtcaatg cggtcatggt
     2581 cctcgccgcc aaaccagagc tcgccctgtt tggtcacaaa caccgaactc gcggcctgac
     2641 tgctgttgcc agcgccgtct ttggcgctga ccaccagttc cacttcaccg gcctggttca
     2701 ggctggtttc gcacatcagc agaccgcgtg catcactctt gccgctgcac acgctgccaa
     2761 gctctttgac gctggtcttg ttgtcgtagc tgtaaaagcc acccaccatg cgtttgcggc
     2821 tgctggtggt gatgcgggcg gtggctttga cctccagtgc cacatcggct agcggtttgc
     2881 cgctggtgtc tagcgccagc gcttggaact tgattttttg gctgctcgac acccagcctt
     2941 cggtcttgat gcctgccacc acgccggacg gccacagggt ctgggtgctg cgaatggtct
     3001 gcacctcgcc gttcgggtcg gcgtagctgg cttccagcac caggtcttgc ggctcgcggc
     3061 tggccgggag cttgtcgatg gtcaacttgc cagcgccgtt cttgtccagc gtcagcgcca
     3121 gcttgtcggc caccacccgg ttgtcctggc tggcgctttc ttcttcggcg tgttcggtgc
     3181 tgctggcctg gtcggtgcgc ggcggtgaaa agctgaaggc atcaaagtcg ctgaaatgga
     3241 tgtacttgct gcgcaccatg gccgacaccc gcaccggcaa gctgctggca ccgccgcccg
     3301 ccacatagtt gacctgcacc gaaagtggca gcgccttaag gttgaccagg gcctttttgt
     3361 cggtcggggc gatgttgcct tcgagcactg gcaggcggaa ttcttccacc cggaattcac
     3421 cggtgttaaa gctgcggttg ttgtcgctgc cctgcaagcg cacctcgtaa ttgcccagct
     3481 tggctgctgg cggtatggca aagctgcttt cggcactcag gccaccggtg gcggtcttgc
     3541 gccagaccag tggctgtgaa taccgctggc cgctgccgat gtgggtgatt tgcacggtgg
     3601 cgggttgtgc ctgggtcagg ccaaaaccct gtgcggtttc gctgcgcagc aggtgcttca
     3661 tcgacacggt ttcgccaacc cgcagcaggg tacggtcaaa aatggtgtgg gcgcgctcgt
     3721 cggggcgggc ttcctggctg gtgggcaggt tgaagcgcca gggctcgatg ccacggttcc
     3781 agtcgctcca ggtaaaggcc atgtcggcag cacccgcgag ctgcgcgcgg gcgctgacaa
     3841 agtaggcatt gctgtagccg tcttcgcccc ggcaaggggg cgccttgggg ctgatgccgg
     3901 tcagataggc aatgccctgg gcgttggtgc tggcgctggc aatctgctga ccctggcagt
     3961 cggacacccg cacctgtgca ttggcgaccg gctggccctt gtccagggtg gtgacccagg
     4021 ccagcgcgtt ttcgcggccc agtttgaaat gcacccccag gttggtcacc agcgccgagg
     4081 tgcgcacata catggtgcgg ccagcgccgt ggcgctcatc cagcagcgag ctgccgagct
     4141 tttgcgaagc cagctccagc acatgaaaac cgggggtcag gggaatgcca accacctcga
     4201 acgggcgcgg gtcattgctg gcgggcttgg gcaagtcgag tgtcttgacc ccggcccgcc
     4261 cttgcagcag cgacaccatg cgtgcctgca cctcggtctt gtcttcatcg ggcagcacct
     4321 gcggcagtgc gcccttgacc tcacgggcgg caacgctgcg cggcaccgag tagtcgtcaa
     4381 aacgcttgac tttcctgaac caggcaatga tgtcagtatc ggcttcaatc ttcaggtcgc
     4441 tgaccttgcc gttggccggg ctgagcgcct tgatcttcag gtcagcttcc acattgcgca
     4501 aggtgatcgg cagcatggcc acaccatcgg gttcggcaaa gcgctcgacg atgccaaaag
     4561 acgcggcggc aaacttggcc agcggcggca tcgcgccggt gctgaccttg agcggaaaac
     4621 tgtcggcatt gaccagcggg cgccccgagg catccttgaa ttccttgggc agggtcagct
     4681 tgaagctggt ctgctcggca aacaggccct tgaagctcac ccggctcaca acattgtctg
     4741 cgtcgccttc ttcgtcttgt tcaaagctgg gcgaataagt ctgtttgtcc gatgccagtt
     4801 tgatgccttc gagcagcttg cgcgccaccg gagccgtaaa gtgcaggcgc atcgggcgga
     4861 tcggcaggca ggcggcctgg gcgttttcgc gttcgcaggc aaaagtggcg gcaaacggca
     4921 cgcgcacctg aaaaccaaag cgcttctcga ccgagttggc gatgccttgc gggttgcttg
     4981 ccgtgccggg ggtgctgacc cccttgccgt aaaccacctg aactttggcg gaaggggtaa
     5041 gccgccggtt gcacgccagg gtgacgaaac tcaacggggt tttggcggcc tgggtttcga
     5101 gctggtgaaa tttgagcaaa gctgcgcggt ctttgccgtc cacctggcgc accgccacgc
     5161 gctcgccaag accctcgacc gcgcaccagg tgttggccag tacgctggca agggtggccg
     5221 ggccgctcag ttgcaagaca aaaaactgat cctcgtcaat tggcgtgtca gtgcccggcc
     5281 agatctcctg aacaaacggc ccgccactat tgaatttata gctcccagag cccgtcagca
     5341 ctgcgccctt gaccgatttg atgcctgatt tcagcttgat ggtgcaagcc acgccgggtg
     5401 gcaggtcggc ttcaaagtca taaacccact gtcggtcatt gagccagcga ccggtgcctt
     5461 gggtggcttg cgggttactg cactgcaggt caagcggtgc cggggccttg gggtcgccaa
     5521 agttgaccgc actctcgtca aacttcacca ccaactgggt gatacgtgcc acctcgccct
     5581 ggggcgacac actgacgacc tgaaaggcct gcgccagggc agacaaggcc agcaaaccgc
     5641 cagccagaac aatgttgcga aggttcacgc caaatcccct tgagtgaacc cgaactttag
     5701 cctaaagcct gcgctacaag gtcttgcgaa tcagcactcc ggtaaaaaga tagggcaggc
     5761 gcggcgggct gccggcctcg accccgccct ttttttccac gctgatcgcc aggcccctgg
     5821 cttgcgacag gatcttgtca gtaacgggga tttgggcggt cttgattttg gtggtcagca
     5881 cacccagcga cagcggctgg tcttgctcgt caagcgccca taactgcatg ctgtcttcct
     5941 gtccttcctt gacatcgttg agccgctgga tcagcagata gccgtccagc gggtcaaagg
     6001 tgatcagttg cgctggcaga tgcttgtcat cgagcagcac tgccacgtac ttcacctgcg
     6061 gcaccgattt gagcttggtt tgcaggtgga tcagttgcgc cttgaactgc tccatcatgc
     6121 tgacaccggt ggcggctgca atggccagca ggatcaggca gatgatggtg gcaacgcgcc
     6181 agcgggtgac gtggcagggc ggtgtcgggg ccgggttcgg gctgcttgtg gtgtcggtca
     6241 ttgaatcgat ttcgtgtggg tgaagggaaa tgagcggcag ggccttgccg ccgctgcgat
     6301 gatgaagaac tgtccgggcg agctttgatg gactgcggta ccgccccgga gatgaccgct
     6361 atgtcaggtt aacccaagcg ggcctgatgc cgcgcaattt gcgcacgcac ctgctttggt
     6421 gcggtgccac cgagcacgtc gcgtgcgttg agcgagccgc gcaagctcag cacgtcaaac
     6481 acatcctttt cgatgccggg gttgaactct ttgagcactg ccagtggcag ttcggacaga
     6541 tcaacctggt gcgaaatggc cgccttgacc gcatgcgcca cggtttcgtg ggcatcgcga
     6601 aacggcaagc cctttttcac caggtagtcc gccagatcgg tggcggtggc gtagccacgc
     6661 agcgcggcgc gctccatggc ctgcgcgttc accgcaatgc cgccttcctt gatcccgctg
     6721 gccgggttga tttgcccgcc gaccatctcg gcaaagatgc gcaaggtgtc cttgagcgtg
     6781 tccacagtgt caaacagcgg ctccttgtcc tcctggttgt ccttgttgta ggccagcggc
     6841 tggcccttca tcagggtgat caggcccatc aggtggccca ccacccgacc ggtcttgccg
     6901 cgcgcgagct ctggcacgtc ggggtttttc ttctgcggca tgatcgagct gccggtggta
     6961 aagcggtcgg ctattctgat gaaaccaaag ttctggctca tccacaaaat cagctcttcg
     7021 ctcaggcggc tgatgtgcac catgcacagc gcggcggcgg cgctgaactc gatggcgaag
     7081 tcgcggtcgc tgaccgcgtc gagcgagttc tggcacacgc agggcagacc ctgctggtcc
     7141 accatgccca atgacatcgc cacccgctcg cggtccagcg agtaactggt gccagccagc
     7201 gccgccgcac ccagcggcag gcggttgacg cggcggcgca cgtcctgcag gcgttcggca
     7261 tcgcgtgcaa acatttcaac ataggccagc atgtggtggg caaaactgat cggctgtgcc
     7321 acttgcaggt gggtaaagcc gggcatgatc acgtcaacat gctgttctgc caccgccagc
     7381 aaggcctttt gcaggtcggt cagcagcgcg ccaatcaggt cggtctcgtc gcgcagccac
     7441 agccgcacgt cggtggcgac ctggtcgttg cgtgagcgcc cggtgtgcag gcgcttgccg
     7501 gcatcgccca ccagttgggt caaacgcgct tcgatgttca ggtgcacgtc ttcaaggtca
     7561 agctgccatt caaagctgcc ggattcgatt tcgctgctga tctgtgccat gccgcgctcg
     7621 atatcggcgt ggtcctggtt gctgatgatg ccctgcgccg ccagcatctc ggcgtgcgcc
     7681 agagagccgc tgatgtcggc ttgccacagg cgtttgtcaa aaaacacgct cgacgtatag
     7741 cgtttgacca gatcgctcat gggttccgaa aacagggccg accaggcctg ggacttttgg
     7801 tcgaattggt ttgagctcat ggtgttcaat ggtgcaggct gccgggcagc aagcccggga
     7861 tcaacaataa tggtgcgcag tgcgtggtga cgcactgacg tgccggactc agatcgatgg
     7921 tccggcgctg aaagttgtgt accttcacac tggatgggtc aatgcaagat tctcaaattt
     7981 tatcggtgtt ccaggaaccg gccacgctgg catccgatat cgggcagccg ttcatgctgc
     8041 atgatgcgct ggtctttgat gcctgccatg tgggtgtggt gttgcgggca gtgctgtttg
     8101 tcgaggcagt ggtcggtgtg gtgaccatgt ttggtgccag cagcgcggtg cagtggcttg
     8161 gccagatggc gctggtaacc ggggctgctc tgccagctac gttgctctgg ctggttgccg
     8221 cctgcagcct gaagagactg ctggcgcgcc tgagcccgac ggcgcagaaa ctggccggtg
     8281 tggccttggg agcggtggcc ggtgcgtatg gctgcggcat gctggttgtg ataggtttgc
     8341 tcgacagtgc cccctggtgg gccagcgcct gcagcggagc catgctgtcg gcggcgctgg
     8401 tggtggcact ggtgctgcgc gccaaaggca agaccccggc ggccactgcg gcacggctgg
     8461 ccgaactgca ggcgcgcata cggccgcatt ttttgttcaa taccctcaac agcgcaattg
     8521 ccctggtgcg cgccgagccg gccagggccg agcgcttgct ggaagacctg agcgacctgt
     8581 ttcgctatgc gctggccgag cagtcggcaa gtgtctcgct ggctgatgaa attgcgctgg
     8641 cgcaacgcta cctggcgatt gagcaggtgc gttttggcga ccgcattcag gtgcaatgga
     8701 cgcttgatgc cagcgccagg caggcccggc tgccaccctt gttgctgcaa cccctgattg
     8761 aaaacgcagt gaaacacggt gttgaaccca gcgccggtgt cacgcacatc aaggtcagca
     8821 ccgagcgccg tggcgccacg gtggtcatca aggtcagcaa cagcatgagt cagccgtcaa
     8881 cagtgcaggc accaggccat gggctggccc tgaacaatgt gcgcgagcgc ctgagcctgc
     8941 tgcatgatgt gcagggcagc tttcgggcgg gttacaaaaa tggcatcttt caggttcgca
     9001 tcgaggtgcc gctgtgatgt tcggctgatc aggaggtgtg atggcgctgc gagttttgat
     9061 cgttgatgac gaagcgctgg cgcgttcgcg cctgtgccgc ctgctggctg actgcaccag
     9121 ccctggtgct ttggtggtgg gcgaggtggc caatgcggcc gatgccatgc aatttgtaac
     9181 ccaccacgcg gtcgatgccg tgctggccga tattcacatg cctggcgctg atggcctgac
     9241 gctggcgcag aatctgcgcg ccctgcccaa tccgcccatc gtggtgtttg tcacggccta
     9301 cgccgagcac gcagtgcagg cttttgaact ggaggcgcag gactacctga ccaagccggt
     9361 acggcttgag cgactccagg ttgcacttca aaaagtagag cgtgccaggc aggcagcaaa
     9421 agggccagag cctgatttga ccgaagaagt gctggtgatt caggagcgtg gccggaccga
     9481 acgggtgccg ctgtctgcgg tgctttacct gaaggccgag ctgaaataca tcacggtgcg
     9541 cacggctggt cacagttaca tcctggaagg cgcgttaagc gatctggaag cccgctacgc
     9601 cagccgcctt atccggattc accgcaacac gctggtcgcc aggcgtgccg tgcgctcgtt
     9661 gcagcgccat cacgatgcgc aagagggcga aggctgggcg gtgcggctcg acggcctggc
     9721 tgagccactg tatgtgtcgc gccgacaact ggtggcggtg cgcgcgttgg tggcggccag
     9781 ggactgatcg ccgcacctga ccagattggc cggctgtagc gacaaccgcg ccacagcagg
     9841 tggctaaaaa gccagcgccc tggcactgac aaagtggcgc gccccgccgg ttatcgggtc
     9901 ggtgaaggcc agcgttttgg ccagcagttg cagcgggcgg gtgtggtcgc tttgtccctt
     9961 tggggtcagc accgggtaga gcccgtcgtt cagaatcggc agaccgagtg ccagcatgtg
    10021 gacccgcagt tgatgccgct gtccggtcac tggcctgagc tggtagcgcg ccagatcgcc
    10081 gcgcacctcc agcacgtcga tctgggtggt ggcgttgggc acgccgtcaa cctcatgctg
    10141 cagcatgaaa tgcccggcct ccttgatgcg gctgtggcgc gtcacaggcc agctcaggcc
    10201 ggcacgccag ggcgcaatcg cttcataggt cttttgcacc tggtgcgcgc tgaacattgc
    10261 acaccaggcg gcgcggctgg ccggttgttt ggaaaacagc accagcccgg cggtgtcgcg
    10321 gtcgatgcgg tgaatcggca ccagatctga caaccccagc ttgcgtttga gccgcaccag
    10381 caaggtctcg gccaggtagt cgcccgacgg caccaccggc agaaaatgcg gtttgtccac
    10441 caccagcagg tggtcgtcct ggtacagcag cacctcatcg aacggaatcg gtacttcgtt
    10501 gggcacttca cggaagtaat acagcagcgt ttggcccggg ccgggcggct ggctggcccg
    10561 aagcggatca atcgccacgc cggtcgcgtc gaaaacatcg ccgcgcctga ttcgttccag
    10621 ccaggtctgg ccggtcacat tgggaaagcg cgccagcaag aagtccagca acgttggcca
    10681 atgattgccc gtcaggctga cacaacttgg gccaacgccg ttgcgcgcag gcggcttgcg
    10741 gtgaggcaac gtcgctttgc ttgtgctcat ggcctgtcct tatgccatct caatccggca
    10801 ggatgccttg ccttgctgtg gctgaccggg cgagcaggca acttcggtcc acctgccaaa
    10861 agcctggtgc cagccagcct caaacccgct caaacaccac atcccacacg ccgtggccga
    10921 gcttgagtcc gcggttttca aacttggtga gtggccggta atgtggcttg ggggcatagc
    10981 cctgaagttg cgggtgcgct gccagcgcgc tgtttttcag cagcggctcg gcgctcagca
    11041 cggcgagcat ctgttcggcg taggcctgcc agtcggtggc gcagtggatg tagcccccca
    11101 ccttgaggcg cgctgccagt tttgccacca gcggtggctg gatcaggcgg cgcttgttgt
    11161 gccgggcctt gtgccagggg tcagggaaaa agatgtggat gccgtccaga ctttgcaagg
    11221 tgagcatgtg gtcgatgacg gccaccgcgt catgcgcgca aatgcggatg ttgcgcaggc
    11281 cctgttcgcc aatgcgcttg agcagcgcgc caacgccgct ctggtgaacc tcgcaacata
    11341 gaaagcgggt ctctggcagc aaggccgcaa tctgcgcggt ggcttcaccc atgccaaaac
    11401 cgatttccaa caccaggggt cgatactttg aatcattttg gcccgcagcc cttatggggt
    11461 ctgcgtaagc agcttccaaa tcaatagcgt ctgactgata cggcagcaag aaccttggcc
    11521 ccagttcctc aaacgcccgg ctttggccat tggtggtgcg gccagcgcgg cgcacaaaac
    11581 tcctgatttc cctggggtag tccacgccag cgggcatctg gcggggtttg ccgggctggg
    11641 ccggatcagt tggcattggt ggcacagcgg gttggggggt gggtagcgtt cacggggcgc
    11701 gattgtagag aggacgggtc aactgccctt ggcggggaac cgaaaaaaga cagcaaacgc
    11761 tctggccgtt ccaacaggaa gttgctaatg ggtcaagcag aatagcttgg acaaagcagg
    11821 tcgtgatggc aagtgcaagg ctacctggca aaatatttac gggattcgtt ggtttgctga
    11881 aagaacatgt gggcaagaaa ttaaccttga tcattgataa tgcgtctatc cacaaggcaa
    11941 aggcaaccag ccacctcatg gacgttttag aaaaacaagc tgttactctt tatttcttgc
    12001 caccctaaag tccagaactt attagatatt ttttcgtcat tgttaaagag aaaatattac
    12061 acccttctgc aaaatttata ttttttgagt ggaaatacta tggatgaact cgataaacag
    12121 gataagcgaa ttgctgcaat cttaaatgaa gatgagccac ccgaagtcac ctatgaaaca
    12181 ttgcgaaagt atgtaactca tctgagagcc aacatagtgt taccctgctc tttgactgga
    12241 atcgaggact tcgattggga agagcgttat gtcattggtg gtggtgataa aaaggaatac
    12301 gaaaagctca agaagacccg tccttcttat actgatgtct tttcgctgtt gagttttagc
    12361 gatgacgttg atgaaatgta tgggatattt gtcaaagtta agcgagcatc tgacaaaaaa
    12421 cagtttaccc ttccgttggc tgatttggaa gttattgata ttaattcttc caactttcag
    12481 ttggtgaatg atttttccgt ctggtttgtc aacaacagtt aagccattgc catcaaatta
    12541 gatacgtttg aaaagcaaat ttatactttc ccccaaaatt attgtgaatt tcgtccacat
    12601 cagtttctaa ggcttaactg ttgcggccct ttgctgacat cgaagtgaat ttcatcaagt
    12661 gccacaattt ttcaattcta ttaagttctg gactataggg tgacaagaaa tgaagagtaa
    12721 tgagcagccc aggccggcat ctgcgtcgcc cagacccagg tcattgccct ggtggcagcc
    12781 aatgccgtgg ccattcggcg attcgaccgt gtggacggcc agcggattca cagtgtttcg
    12841 gcgggcaccg ctatccgggc ggcaactgtg gcgggtaccg aaccagaaat gggatacccg
    12901 caactcgccc gaatccttcg cagaatcggt ctaacccagg gcgacgcgca tctgaccgat
    12961 gctcaggagc tgtttcgccg aatggtgttc aacatcttga ttgacaacgc cgacgaccat
    13021 gagaagaatc acgcgctttt ggtcgtggcc cctgccgcca atggacggct aaggttggca
    13081 ccggcttatg acgtactccc caccaactcc gggcagggct atcaagagtt catctgcggt
    13141 gcttttggac gagagtccac gctggccaac gcgatgtctc agtgcgatgc gtttggcctt
    13201 caaccggcac aggccgccgc gcaagtcgtc aaagtcatcg gggtggttaa cacctggcgc
    13261 gcgcattttg acgccatcag ggtatccaaa agcgacctgg agggcttggc agagcgactc
    13321 gacggtgacg agttactggg ccatcggcag aggcttgatg ccggccagta ccaggtagca
    13381 gctcccaaac gacggccgac aagtccattt cgcaggacat gacgcgtgat tgacaggtcc
    13441 ctggcccggg cctgttcggg caagacggcc acaagtggga accgccatgg caaatccgga
    13501 ggcttttagc ttctgtagaa gatgtcttct tgagtaaaaa tgcgccccaa tcaatgactg
    13561 gggcgcatct tggtcgaatc aagccactat tggcaacttc tttttgaaac ggccaagtaa
    13621 acgctggcca tttgatttgc gcctctgttc attgaaccgg cgcatccatg aatccatttc
    13681 ccggtcaatc cgacatggtg ttgcttcgcc ttgccgcgct gtagcactgt ctgcggcttg
    13741 gcactggatg tcgcatcgat caggaaacgc aatcagaggc tgtttatttc atgtccacgc
    13801 cataaaagga gtggcggcca aacgggctca gtttgaagtc gatgacttcc ttgcgtactg
    13861 gcttcaactg aacggcgtgt gcaatggtga accacggcgc ttgctgcttg aagatcacct
    13921 gggcctgctc gtagagtttg gtacgttctg ccgggctgga aaccactttg gccttttgca
    13981 ccaggtcttc aaacggctgg tagcagaact tggcgacgtt cgagccgttg ttcttggcag
    14041 cgctgcaacc caacagggtg ttcaggaagt tgtccgggtc gccgttgtca ccggtccagc
    14101 ccagcatgcc catctg
//
LOCUS       DAOSLL010000036        14112 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4413_length_14112_cov_4.564274, whole genome shotgun sequence.
ACCESSION   DAOSLL010000036 DAOSLL010000000
VERSION     DAOSLL010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 14112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14112
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4413_length_14112_cov_4.564274"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="PLB25_02695"
     CDS             complement(<1..660)
                     /locus_tag="PLB25_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40532.1"
                     /translation="MKKLTVKLQNCYGIQFLEHTFIFNKYPRDTNSYAVYAPNGLMKT
                     SFSKTFQDLSAGNIPKEERYNRESTCEVEFDGTAIPKEFIYALKSEIDIREESQSITD
                     ILVNPENKLQYDRILFDLNISKEKLKTSLQKSSKIKKKDIEEFILRDWNQIDFPTCIL
                     SILETPSTEDLGSFEYNTIFDPKAIEVFNSPEFIAKAKDFNDRYQELFDQAGTIYTKN
                     VF"
     gene            complement(915..1988)
                     /locus_tag="PLB25_02700"
     CDS             complement(915..1988)
                     /locus_tag="PLB25_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOE40533.1"
                     /translation="MNETTRNVVPAPADILHAELRALIASSRQRLAGAVNAELTRLYW
                     NVGHRLRTEVLGGADRAKYGDQLIDRVGDQLAQEFGRGFEAKNLRRMVQFAVAFPDTE
                     KVASLMRHLSWTHFLQLLPVKTDAARWFYAQQSVESRWGVRELHHQIERKAFERTELA
                     ALQTNTPVQAEPDVAANTNPAQVFKDPYFLDFLGLRQGHDEADLEAAILRQLEAFILE
                     LGRGFAFVERQKRMVIDGDDFYLDLLFYHRRLRRLVAIELKLGRFKAAHKGQMELYLK
                     WLDKHERQPGEEAPIGLILCAESSREQVELLQMHKDGITVAEYWTELPPKAELEQKLH
                     SALLEARERLARRGVLLGDLDDE"
     gene            complement(2002..2547)
                     /locus_tag="PLB25_02705"
     CDS             complement(2002..2547)
                     /locus_tag="PLB25_02705"
                     /inference="COORDINATES: protein motif:HMM:NF019976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease-related domain-containing protein"
                     /protein_id="HOE40534.1"
                     /translation="MATLIPAISTCRFDTTGERRLAERLEQKLDDDYWMWHNVPVGPT
                     NVHPDFVVMHPRRGLLVLEVKDWKRATIQSADKQSWTIIPDGVPKTVINPLEQARQYA
                     HQVVDALKRDPQLTQPDGPHQGQIAFPWSYGVVLHNIMPASGEDEKEAARVFYVAATR
                     ATQSLVIGVGGDGGFGSKLGT"
     gene            complement(2852..3346)
                     /locus_tag="PLB25_02710"
     CDS             complement(2852..3346)
                     /locus_tag="PLB25_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40535.1"
                     /translation="MKALTVEQQVAMLRPGMSFAAHMHHWLLAGAAMGVMLAVIFWHP
                     IPLMIAAFLGLVGFSEQRAGPNIVAAIVAYDSGTPSNGEVAIAITCWDTDKHYHATVR
                     EPGHPNWKYEFVPQGWQPASRSCLAQIWRSGSDGSPVLAVVEQGILIPRHEPKLAGKL
                     RPSS"
     gene            complement(3679..5001)
                     /locus_tag="PLB25_02715"
     CDS             complement(3679..5001)
                     /locus_tag="PLB25_02715"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE40536.1"
                     /translation="MNASATSQVLGPSPVVLVIEDEPGDACLIRQQLLEHGETFLVHL
                     ADSLAGARALIDEQGLRPDVVLLDLNLPDSSGTATVERCRLLVEAPIVVLTGLDDVLA
                     TQVAIQSGAEDYLTKGGDASALRRAVRYAMLRHQRDADTRLAATVFAHAREGIMVTRS
                     DGTIMDVNQAFTRITGYQRDEVLGQNPHLLSSGRQSKEFYAAMWLDLQQQGYWSGEVW
                     NRRKDGLIYAAMQTISAVRNAAGAARQYVALFSDITALKAHEHKLEHIAHFDVLTDLP
                     NRVLLADRLRHGMAQAQRRGKLLAVVYLDLDGFKRVNDSHGHAAGDMLLMTLATRMKQ
                     ALREGDTLSRIGGDEFVAVLIDLEDASACLPMLARLRDAAAQPIPFGNISLQVSASLG
                     VAFYPQTGELDADQLMRQADQAMYQAKVAGKNRYCFFDARLGGLTEGD"
     gene            complement(4998..5462)
                     /locus_tag="PLB25_02720"
     CDS             complement(4998..5462)
                     /locus_tag="PLB25_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006028753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE40537.1"
                     /translation="MTHATTLPFVILLAEDEPADAHLVRTALTENRIRADLRHVQDGR
                     EALEYLRQEGSRFADAPRPDLILLDLNMPRMDGRECLAALKQDPALCDIPVVILTTSE
                     VERDVLASYRLGASGFISKPVDMAQFIEAVGNLGNYWINLVRLPGHPVEVVK"
     gene            complement(5486..5926)
                     /locus_tag="PLB25_02725"
     CDS             complement(5486..5926)
                     /locus_tag="PLB25_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE40538.1"
                     /translation="MSQAKKLNVLLVEDEPADAHLVRLAFAEGQVPVDLHHVPDGVEA
                     FAFLRREGAYANVPRPDLILLDLNMPRMDGRQFLKKIKLDAALRSLPVVVLTTSDAER
                     DLLDSYQHFAAGFIVKPVDVDDFIKLASGIGDYWVNIVRLPEQN"
     gene            complement(5923..7236)
                     /locus_tag="PLB25_02730"
     CDS             complement(5923..7236)
                     /locus_tag="PLB25_02730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE40539.1"
                     /translation="MKIYLRLAAWVLAWFCAWPAWAEVAKPEFDRIFHDFEVTMLLID
                     PASGQIVDANLAAARFYGFEQARLRSMTIQQINTFTPQQVAAERQLAQTEGRNYFIFR
                     HRLANNDVRTVEVFSRPFDFAGRKLLFSMVNDITPGRHRQQDLWHYQQRLEEMVEVQV
                     REIERVKHQQMALALAGLLVQALVIAWLVISVRRRRSLERERETLLTSLQARNQELSR
                     LGEVMAHHFQEPTRRLASFSQRLLAKSELARDEDSRLSLNFIHTESQRLSALVGDAQR
                     YLALDKNQVSAAGVADCAAVLRQCIEDAGDTTAGADIVLHSPLPRLQMAEKSLRGLFA
                     ILFDNALRYRHPQRPLRIEVGASTKGQRAVFRVADNGSGIAPEYRSQVLGLFTRLVPS
                     SVPGTGMGLALAYKITSLSGGDLHIEDGLDGGACIVFDLPLETLE"
     gene            complement(7233..9806)
                     /locus_tag="PLB25_02735"
     CDS             complement(7233..9806)
                     /locus_tag="PLB25_02735"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE40540.1"
                     /translation="MSTSSVSRNSLSDTAAPQAIAPQRQLPFASSPSAPIVRQQIRWL
                     LVALALLLALLAYFVQDRYRQAQADAASDTRNLVQLIESHLSSDFARVDGILGYIASG
                     FSEDDLHPALPNAAQVVRQQRLAALQASFSMIGGLSVFDAQGALLYASDPSLKGINIA
                     DRPHFQRLRDDPLAQLVFSEAQVARTTGRWSIVMVRPLHDRQGGFLGTVNAVIALEQI
                     GKLFGTVDVGANGGILLRNSETFKLIQRMPRLNEKDFNQPLPKDNATRQRMDAGKRAG
                     TLIFTASTDSVERLASFKRMDAYPFYVQVALAKSDYLADWYQEAAAVASLAVLLLLGC
                     ALTIRRWQKSAAVIAAASHQLAYRQALSAGLFEQSGFLAGILDASGHLLEVNQTALAT
                     IGLRHDQVVGQVFADTPWWQRAEDRSALHATLQAAAQGKAGSFEAVHRSVSGTDMTVL
                     FHAAPVLVGEDRYIAVTGIDISASKAAEQELAQQGQRLSNILWGTGVGTWEWNVQTGE
                     TRFNARWAELIGCTLDELAPVSIATWMKYAHPDDLAASGTALQQHFAGELDHYECETR
                     MRHKLGHWIWVLDRGKLVSRSADGQPEWMAGTHWDITKRKQAEQALQQQTEALARSNA
                     ELEQFAYVASHDLRQPLRMINSYLQLIERALVAQLNDDTREMMHFATDGAKRMDQMLV
                     SLLEYSRVGRKGEPLALLDSLDAVDEALHFLAPAIQEAQATVRLSGDWPQLLASRDEF
                     TRLWQNLIGNAVKYRAPDRAPEIDITVTPDAEGAGWRFCVADNGIGIDPAQFDRLFKV
                     FQRLHTRDRYQGSGIGLAVARKIVQRHGGRIWVESAGPGQGCRFCFDLPARQPDTPVS
                     P"
     gene            complement(9919..10152)
                     /locus_tag="PLB25_02740"
     CDS             complement(9919..10152)
                     /locus_tag="PLB25_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40541.1"
                     /translation="MTIKLIATRAYSKRATGQFLINFRPGTARQPSASDGTDVGLALL
                     RRIAKSGGRAWIETTAGGGCGVLLKPPQPQANP"
     gene            complement(10163..12835)
                     /locus_tag="PLB25_02745"
     CDS             complement(10163..12835)
                     /locus_tag="PLB25_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE40542.1"
                     /translation="MTQTLNETPTILVIEDDPGDFGLVKAYLRLAGFCASGEASGPVW
                     ATTLAEGIAKAQQAAPDIVLLDLSLPDSAGLATLQTLRAALPSAPIVVLTGNDEKTLA
                     LATLESGAQDFLVKGQFDPDALGRAIRHARVRERLEQRLAHSQRLYAALSQCNQAIVR
                     CSSEPELLEQACRIAVDFGGFKMAWIGLVEPGSQWVRPVTSFGSGTAYLQNIEISVDA
                     NQPQGRGPTGTAMHCQQPVWCQDFLQDPLTAPWHEVARDFGWRSSAALPLNRQGVVIG
                     SFNLYASECHAFDDAARTLLTEMASDISFALDNFVKEAQRKQAAVALRESERRMRSFF
                     ENAPVGIFLTSRAGKILYVNPAMAVILGYASSAELMDIVNRRSIAEVLYEEPLTRNTI
                     MQEVGQSPDRWLTLENRFRRKDGRVIDAILSVGEETDPTSGMVNLLGVVIDISQSKQA
                     SEHILWLSRFDQLTGLANLSLLGDRFQHAMSMAQRSQLALAMMTLDLDHFKNINDTLG
                     RHIGDAMLTEVARRIQSVVRDEDTVSRQGGDEFILLLPTTDAAGAAHLAERLLAAIAM
                     NYQIDAMELLTTPSIGIAMYPTDGSDFETLTKNADVAMYRAKQDGRNCFRFFTAEMQT
                     RSTRTLHLENALRRSLERGQLSLHYQPQVALASGRVIGAEALLRWHHPELGSVSPAEF
                     IPVAEASGQIVQIGIWVLRSAVRQMKAWLDLGLAGAGAPFVMAVNLSVVQFRHPRLVE
                     QVMQILQEEQLAPEYLELELTEGVALSDPLGAITVMNALHAQGIRMSIDDFGTGYSSL
                     NYLKRFKVYKLKIDQSFVHDITHDPDDQAIVNTIISMAARLGLQTIAEGVETAGQLQF
                     LRDSGCDEVQGYFFSKPLPAPAFEAFLRQSPSSA"
     gene            complement(12857..13315)
                     /locus_tag="PLB25_02750"
     CDS             complement(12857..13315)
                     /locus_tag="PLB25_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004188466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE40543.1"
                     /translation="MTTEKSSFVILLAEDEPADAHLVKVAIAENRILAELHHVLDGRE
                     ALDYLQRLGPRYAGAPRPDLILLDLNMPRLDGREFLAAIKQLPDLRDIPVVVLTTSEV
                     ERDVVAAYDLGAAGYITKPVDVAQFMAAIRALGDYWIDLVRLPKGNGDGG"
     gene            complement(13312..>14112)
                     /locus_tag="PLB25_02755"
     CDS             complement(13312..>14112)
                     /locus_tag="PLB25_02755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE40544.1"
                     /translation="WVSHVCQPVFDPAGQPLGLRGSNRDITARKEVEAALARTHADLQ
                     RFAEVTAHHLQEPARRIANYAERLGKQLAGRIDDAEAQLSLDYIGQQARRQQNLLRDV
                     ERYLAADQPRGKVNWVDVQMSVNALLARWKTRIDQAGATITLGQLPPAWIDPPRLGDL
                     FEVALDNALNHGRSAQPLLILIEGMHDGHKTCYIVSDNGPGVELAYRERIFRVFERLA
                     PASEGTGTGIGLAILRRIAESCGGTAWIEERIGGGARLVIELAKEKRT"
BASE COUNT         2733 a         4303 c         4252 g         2824 t
ORIGIN      
        1 gaaaacattt tttgtataaa ttgtccctgc ttgatcgaaa agctcctgat agcgatcatt
       61 aaaatccttt gccttagcga taaattcagg actattgaac acttcaatcg cttttggatc
      121 aaaaatcgtg ttgtattcaa atgaccccaa gtcttcagtt gaaggtgttt caagaatact
      181 tagaatgcag gttggaaaat cgatctgatt ccaatctcgc agaatgaatt cctcaatatc
      241 ttttttcttg attttcgatg atttttgtaa agatgttttt agtttttctt ttgaaatatt
      301 caaatcaaac aaaattctgt catattgtaa cttgttttcc ggattaacta gaatatcagt
      361 aatggattga ctttcttctc gtatgtcaat ttccgatttc agcgcatata tgaactcttt
      421 aggtatcgca gtaccatcaa attcaacttc gcaagtcgat tcgcggttat agcgttcttc
      481 ctttgggata ttgccagccg aaagatcttg aaatgtcttt gagaaagatg ttttcattaa
      541 gccattcgga gcgtacacgg cataggaatt tgtgtctctt ggatatttat tgaagataaa
      601 agtgtgctct aagaattgaa tgccatagca gttttgcaat ttaacagtga gttttttcat
      661 gggaattttt tcgtggttat tgtgtctttg gacattgttt tgaactcgga gcttgggtag
      721 ctttcattca cgaagtcgga aacttcaagc caaacccccg accccctccc ccatcacccg
      781 tccctacgta gcccttgtcg ccgccacata aagcacctgc ggggcctgtt gtccccttcg
      841 cccttggaat actgcgcccc actccgggca gtgctaccac cgggaactcc aggcctttgc
      901 tgtccttcat gtgatcactc gtcatccaag tcccccaaca acaccccgcg ccgtgccagc
      961 cgctcacgcg cttcaagcaa agctgaatgc agcttttgct ccagctctgc tttgggtggc
     1021 agttcggtcc agtattcggc cacggtgatg ccgtctttgt gcatttgcag cagctccact
     1081 tgttcgcggc tggattcggc gcacaggatg aggccgatgg gggcctcttc gcccggctgg
     1141 cgttcgtgtt tgtccagcca tttcagatac agctccatct ggcctttgtg cgcggctttg
     1201 aagcggccca gcttgagttc gatggccacc aggcggcgca ggcggcggtg gtagaacagc
     1261 aagtccagat aaaagtcgtc gccgtcgatg accatgcgct tttggcgctc tacaaatgca
     1321 aagccccggc ccagttccag aatgaaggct tccagttggc gcagtatggc ggcttccaga
     1381 tcggcctcgt cgtggccttg gcgcaggccc agaaagtcaa ggaagtacgg gtctttgaag
     1441 acttgggcgg gattggtgtt ggcggcaacg tccggctcag cctgaacggg ggtgttggtc
     1501 tgcaaggcgg caagctcggt gcgctcgaag gccttgcgtt cgatctggtg gtgcaactcg
     1561 cgcacgcccc agcgagattc cacgctttgc tgggcataaa accaacgggc ggcatctgtt
     1621 ttgacgggta atagttgcag aaaatgcgtc cagctcaaat gtcgcatcag tgatgcgact
     1681 ttctctgtat ccgggaaagc cactgcaaac tgcaccatgc gacgcaggtt tttggcttca
     1741 aagccccggc cgaactcttg cgccagttga tcgcccacgc ggtcaatcaa ctggtcacca
     1801 tatttggcac ggtccgcgcc gcccagcacc tcggtgcgca gcctgtggcc cacattccaa
     1861 tacagccgtg tcagctccgc attcacggca cccgccagcc gctggcggct gctggcaatc
     1921 agcgcgcgca gttcggcgtg caggatatcg gcaggcgcag gcactacgtt gcgtgttgtc
     1981 tcgttcatgt tgtttttctg gtcatgttcc cagcttacta ccaaacccac catccccacc
     2041 caccccaatc accaaactct gcgtagcccg cgtcgccgcc acataaaaca cccgcgccgc
     2101 ttctttttca tcctcccccg acgcgggcat gatgttgtgc aacaccacgc cgtagctcca
     2161 ggggaaggca atttgtccct gatgcgggcc atctggctgg gtcagttgcg ggtcgcgctt
     2221 gagggcatcc accacctgat gcgcgtactg gcgggcctgc tccagcgggt tgatcacggt
     2281 tttgggcacg ccatcgggga tgatggtcca gctttgtttg tcagcgctct ggatggtggc
     2341 ccgcttccag tctttgactt cgagcaccag caggccacgg cgcgggtgca tcacgacaaa
     2401 gtcggggtgg acattggtgg ggccaaccgg cacgttgtgc cacatccaat agtcatcgtc
     2461 gagcttttgc tccagccgct cggccaagcg gcgctcgcca gtggtgtcaa aacggcaggt
     2521 gctgatcgcc ggtatgaggg ttgccatgaa tgttgtcgcg tccctgtgat gttctgggcc
     2581 gattatcggc catgccactg tgcgtaagat ggtcagtgat ggtgcccgtg cgcccacgtg
     2641 aacgtggcca tgggcatgag cgccagacaa gtgctgtggc aggtggaggt gccgctggcg
     2701 ctgccggtaa ggctggccgg ggtctcaggc gatcaagccc gcgcctgtga gttgcacaaa
     2761 taattggcct ctagccctta tgccataagc gctgatagct actacaagta tagcgaatat
     2821 gagcctcacc aggccgccgg ggaagcagcc ttcagcttga cgggcgcaat ttgccagcca
     2881 gctttggctc atggcgagga atcaggatgc cttgctcgac aaccgccagc accggcgacc
     2941 cgtcactgcc tgagcgccag atttgcgcca gacaactgcg gctggccggt tgccagccct
     3001 gtggcacaaa ctcatatttc cagttagggt ggccaggctc gcgcaccgtg gcatggtagt
     3061 gcttgtcggt gtcccagcag gtgatggcga tggcgacttc cccgttgctt ggggtgccac
     3121 tgtcgtaggc aacgatggct gccacgatgt tgggaccggc gcgctgctcc gagaaaccca
     3181 ccagacccag gaaagcggca atcatcaacg ggatcggatg ccaaaagatc acagccaaca
     3241 tgacacccat tgcagcgcca gccagcagcc agtggtgcat gtgggcggca aagctcatgc
     3301 ccggcctgag cattgccacc tgttgttcaa ccgtaagcgc cttcatcgga tcaagctgga
     3361 ttggtctttc ggacgcgccc gagcgggcct ctggcagcgc gtccgggcag ggtgaaagcc
     3421 gcggctccaa acgtgcctgc gcgcgtttgg acggcttgag gctggccagc cttgtggcat
     3481 cgctgcgccc tgcaggggcg acgacgacgc acagctcttg cccgcgggga ggcgcaacga
     3541 ccccaggcgc ggtgcgtttg gttcagatga gtgcgcggcg ctgtcaccca atgggtgatg
     3601 cccaatatgg ccaatgacgt caagtctcat gaggactttc agatcaaaac aaacggtcaa
     3661 ccgaagaatc cagattcttc agtctccttc ggtcaagccg cccagccggg catcaaaaaa
     3721 acaatagcgg ttcttgcctg ccaccttggc ctggtacatg gcctggtcag cctggcgcat
     3781 caactggtcg gcatcaagtt cgccggtctg cgggtaaaag gcaacaccca ggctggccga
     3841 tacctgcaaa ctgatgttgc caaagggtat cggctgggcc gccgcatcac gcaggcgcgc
     3901 cagcatcggc aggcaggcgc tggcatcttc cagatcaatc aacaccgcga caaactcatc
     3961 gccaccaatg cgcgacaaag tgtcgccttc gcgcagcgcc tgcttcatgc gggtcgccag
     4021 ggtcatcagc aacatgtcgc ccgccgcatg gccatggctg tcgttgacgc gcttgaagcc
     4081 gtccaggtcc aggtacacca ccgccagcag tttgccgcgc cgttgcgcct gcgccatgcc
     4141 atggcgcagc cggtcggcca gcagcacacg gttgggcaga tcggtcaaca catcaaaatg
     4201 ggcaatgtgt tcgagtttgt gctcgtgcgc cttgagcgca gtgatgtcgg aaaacagcgc
     4261 cacatactgg cgcgccgcac cggccgcatt gcgcaccgcg ctgatggttt gcatggcggc
     4321 ataaatcaag ccgtccttgc gccggttcca gacctcgccg ctccaataac cttgctgctg
     4381 caaatccagc cacatggcgg catagaactc tttcgactgc cgcccggaac tcagcaggtg
     4441 cggattctgg cccagcacct cgtcgcgttg ataacccgtg atgcgggtaa aggcctggtt
     4501 gacatccatg atggtgccat cggagcgggt caccatgatg ccctcgcgtg cgtgggcaaa
     4561 aacagttgcc gccaggcggg tgtcggcatc gcgctgatgt cgcagcatgg cgtagcggac
     4621 tgcccggcgc agcgcgctgg catcgccgcc cttggtcagg tagtcctcgg cacccgactg
     4681 aattgcaacc tgggtggcca gcacgtcgtc aagcccggtc agcaccacaa tgggtgcctc
     4741 caccagcaag cgacagcgct caaccgtggc ggtaccgctg gagtccggca ggttcaggtc
     4801 cagcagcacc acgtcggggc gcagaccctg ctcatcaatc agagcccggg caccagccag
     4861 agagtcggcc agatgcacca gaaacgtttc gccgtgctca agcaactgct ggcgaatcag
     4921 gcaggcatca cccggctcat cttcgatgac cagaaccacc ggtgacgggc caagtacctg
     4981 ggatgttgca gatgcgttca tttgacaacc tccaccgggt gccccggcaa gcgcaccagg
     5041 ttgatccagt agttgcccaa attgccaacc gcctcgatga actgcgccat gtccaccggc
     5101 ttgctgatga aacccgaggc acccaggcgg tacgatgcca gcacatcgcg ctcgacttcc
     5161 gaggtggtca ggatcaccac cggtatgtcg cacagcgccg ggtcttgctt gagggccgcg
     5221 aggcattcgc gcccatccat gcgcggcatg ttcaagtcca gcagaatcag gtccgggcgc
     5281 ggcgcgtcgg caaaacggct accctcttgg cgcaggtatt ccagcgcctc gcggccatct
     5341 tgcacatggc gcaaatcagc gcgaatgcgg ttctcggtca gcgcggtcct gaccaggtgg
     5401 gcatccgccg gctcatcttc ggccagcaga atgacaaagg gcaaggtggt ggcgtgggtc
     5461 atggccgtct tctccagcgt agcgttcagt tttgctcggg caggcgcacg atgttgaccc
     5521 agtagtcgcc aatgccactg gccagtttga taaagtcgtc cacatccacc ggcttgacga
     5581 tgaagcccgc agcaaagtgc tgatagctgt ccagcagatc gcgttcggca tcagaggtgg
     5641 tcagcaccac caccggcagg ctgcgcaagg cggcatcgag cttgatcttt ttcaagaact
     5701 gacgcccgtc catgcgcggc atgttgaggt caagcaagat caggtccggt ctcggcacat
     5761 tggcataagc accctcgcgg cgcaaaaagg caaacgcctc aaccccgtcg ggcacatggt
     5821 gcaaatcgac cggcacctgc ccttctgcga aagccaggcg caccagatgg gcatcggcgg
     5881 gttcgtcctc gaccagcagc acattgagtt ttttggcttg gctcattcca gagtctccag
     5941 gggtaaatca aaaacgatgc aggccccgcc gtcgaggcca tcctcgatgt gcaaatcacc
     6001 gcccgacagg ctggtgatct tgtaagccag cgccagcccc atgccggtgc cggggacgct
     6061 gctgggcacc agccgggtga acaggcccaa cacctgcgag cggtactcgg gggcaatgcc
     6121 gctgccattg tcggcaacac gaaacaccgc ccgctggccc ttggtgctgg caccaacctc
     6181 gatgcgcaag ggtcgctgcg ggtgacggta acgcagtgca ttgtcgaaca ggatggcaaa
     6241 cagaccgcgc agggattttt ctgccatctg caaccggggc agtggcgagt gcagcacgat
     6301 gtcggccccg gcagtggtgt caccggcatc ttcgatgcac tggcgcaaca cggcggcgca
     6361 gtctgccacc ccggcggcgc tgacctggtt cttgtcgagc gccaggtagc gctgggcatc
     6421 acccaccagc gcggacaggc gttgtgattc ggtgtggatg aagttcaacg acaggcggct
     6481 gtcttcgtcg cgggccagct ctgatttggc cagcaatcgc tgactgaagc tggccaggcg
     6541 gcgggtcggc tcctgaaagt ggtgcgccat cacttcgccc agacggctga gttcctggtt
     6601 gcgtgcctgc aggctggtca gcagggtttc gcgctcgcgt tccaggctgc gacggcgccg
     6661 gacgctgatc accagccaag caatcaccag cgcctgtacc agcagaccgg ccagcgccaa
     6721 agccatctgc tggtgcttga cacgctcgat ttcgcgcacc tggacttcga ccatctcttc
     6781 gagccgttgc tggtaatgcc acaggtcttg ctgccggtgg cggcccggcg tgatgtcatt
     6841 gaccatggag aaaagcagtt tcctgccagc aaaatcaaac gggcgggaaa acacttcgac
     6901 agtgcgtacg tcgttgttgg ccagccggtg gcggaatatg aagtaattgc ggccttcagt
     6961 ctgcgccaac tggcgctctg ctgccacctg ttggggcgta aaggtattga tctgctgaat
     7021 cgtcatgctg cgcaggcgcg cctgctcaaa gccgtaaaag cgcgcagcag ccagattggc
     7081 atcaacgatc tgaccgctgg caggatcaat cagcagcatg gtcacctcaa aatcatgaaa
     7141 gatccggtca aattccggct tggccacctc agcccacgca ggccaggcac aaaaccaggc
     7201 caacacccat gccgccaggc gcaagtagat tttcatggcg acaccggtgt atctggctgg
     7261 cgcgctggca agtcaaagca aaagcggcaa ccctggccgg gcccggcaga ttcgacccag
     7321 atgcgcccgc catggcgctg cacaatcttg cgcgccaccg ccaggccaat gccgctgccc
     7381 tggtaccggt cgcgcgtgtg caggcgctgg aacaccttga acaggcggtc aaactgcgcc
     7441 gggtcgatgc caatgccgtt gtcggccacg caaaaccgcc agcccgcacc ttcggcatcg
     7501 ggcgtgacgg tgatgtcgat ttcgggcgcc cgatccgggg cgcggtattt caccgcgttg
     7561 ccgatcaggt tttgccacag gcgggtgaat tcatcgcggc tggccagcag ttgcggccag
     7621 tcgcccgaca gccgcactgt ggcttgtgcc tcttgaatgg ccggggccag aaagtgcagc
     7681 gcttcatcca ccgcatcaag gctgtccagc agggccagcg gctcgccttt gcgcccaacg
     7741 cgggagtatt ccagcagcga caccagcatc tggtccatgc gcttggcacc atcggtggca
     7801 aagtgcatca tctcgcgggt gtcgtcgttg agctgggcga ccagggcgcg ctcaatcagt
     7861 tgcaggtagc tgttgatcat gcgcagtggc tggcgcaggt cgtgcgaggc gacataggca
     7921 aactgctcca gctcggcatt ggagcgcgcc agcgcttcgg tctgctgctg cagggcttgt
     7981 tcggcctgct tgcgcttggt gatgtcccag tgcgtgccgg ccatccattc gggctggcca
     8041 tcggcgctgc ggctgaccag cttgccccgg tcaagcaccc agatccagtg gcccaacttg
     8101 tgccgcatgc gggtttcaca ttcgtaatga tcaagctcac cggcaaagtg ctgttgcagc
     8161 gcggtgccag aggcggccag atcgtccggg tgggcatatt tcatccaggt ggcaatgctg
     8221 accggggcca gttcatccag ggtgcagcca atcagctcgg cccagcgcgc attgaaacgg
     8281 gtctcgccgg tctgcacatt ccactcccag gtaccgacac cggtgcccca caggatgttg
     8341 cttagccgct ggccttgctg tgccagttct tgctcggcgg ccttgctggc gctgatgtcg
     8401 atgccggtca cggcgatgta gcgatcttca ccgaccaaca ccggtgcggc gtgaaacagc
     8461 acggtcatgt cggtgccgct gacgcttcga tgaaccgcct cgaaactgcc cgccttgccc
     8521 tgggcagcgg cttgcagcgt ggcatgcagc gcggaccgat cttcggcgcg ctgccaccac
     8581 ggcgtgtcgg caaaaacctg ccccaccacc tggtcgtgac gcaggccgat ggtcgccagc
     8641 gccgtctgat tgacttcgag caagtgtccg gatgcgtcca gaatgcccgc cagaaagccc
     8701 gattgctcaa acaggccagc agacagcgcc tgccgatagg ccaactggtg gctggctgcg
     8761 gcgatgacgg cggcactttt ctgccagcgc cgaatcgtca aggcgcagcc cagcaacaac
     8821 aaaaccgcca ggctggccac agcagcggcc tcctgatacc agtcggccag gtagtcgctt
     8881 ttggccagcg ccacctgtac atagaaggga taagcgtcca tccgcttgaa gctggccagg
     8941 cgctcaacac tgtcggtgct ggcggtgaag atgagcgtgc cagcgcgctt gccagcatcc
     9001 atgcgctggc gcgtggcatt gtccttgggc agtggctgat tgaagtcttt ctcgttaagg
     9061 cgcggcatgc gctggatcag cttgaaagtt tcgctgttgc gcaacaaaat gcccccattc
     9121 gccccgacat caacggtgcc aaacagcttg ccgatttgct ccagcgcgat caccgcgttg
     9181 acggtaccca aaaaaccgcc ctgacgatca tgcaggggac gcaccatgac gatcgaccac
     9241 cgcccggtgg tgcgcgccac ctgggcttct gaaaacacga gctgggccag cggatcgtcg
     9301 cgcaggcgct gaaagtgcgg ccggtcggca atgttgatgc ccttgaggct cgggtccgag
     9361 gcgtaaagca gcgcgccctg ggcatcaaac acactcagcc cgccaatcat cgaaaagctg
     9421 gcttgcaggg cagccaggcg ctgctgccgg acgacctgag cggcattggg cagcgccggg
     9481 tgcagatcgt cttcgctgaa tccgctggca atgtagccca ggatgccatc gacgcgggca
     9541 aagtcgcttg acaagtggga ctcgatcaat tgcaccagat tgcgcgtgtc tgaggcggca
     9601 tcggcctgcg cctggcgata gcgatcctgc acaaaatagg ccagcagggc cagcaacagc
     9661 gccagcgcaa ccagcagcca gcggatctgc tgccggacaa tgggggcgct ggggctgctg
     9721 gcgaacggca gttggcgctg tggcgcaatg gcttggggcg ccgcggtgtc gctcaggctg
     9781 ttccttgata cgctggaagt gctcatgtcg ttggctgaga atttgtaata aattgacctc
     9841 tagcccttat ggaatatgcg tggtcagcta tttatgtaat agcagaacgc ctttgtttgc
     9901 gggctttggc gggttgcttc atgggtttgc ctgcggctgc ggtggcttca gcagcacgcc
     9961 gcagccaccg cccgcagtgg tttcgatcca ggccctgccg ccgctcttgg caatgcggcg
    10021 cagcagcgcc aggccaacgt cggtgccgtc gctggccgac ggctgccgtg cggtgccggg
    10081 tctgaaattt ataagaaatt ggcctgtagc ccttttggaa taagcgcgag tagctattaa
    10141 ctttatagtc atgtgagatc cttcaggcgc tgctgggcga ctggcgcaga aaagcctcga
    10201 aggcgggcgc cggcagtggc ttgctgaaaa aatagccctg cacctcgtcg cagccactgt
    10261 cacgcaaaaa ctgaagctgg ccggcggtct ccaccccctc ggcgatggtt tgcagaccaa
    10321 gccgggcggc catgctgatg atggtgttga cgatggcctg gtcgtccggg tcatgggtga
    10381 tgtcatgcac aaacgactgg tcgatcttga gcttatagac cttgaagcgc ttgaggtaat
    10441 tcagcgacga gtagccggtg ccaaagtcgt caatcgacat gcggatgccc tgcgcgtgca
    10501 gggcgttcat caccgtgatg gccccgagtg ggtcgctcag cgccacgccc tcggtcagtt
    10561 cgagctccag gtattcgggc gcgagctgct cttcttgcaa aatctgcatc acctgctcga
    10621 ccaggcgcgg atggcgaaac tgcaccaccg acaggttgac cgccatcaca aacggtgccc
    10681 cggcaccggc cagccccagg tccagccagg ccttcatctg gcgcactgcg ctgcgcagca
    10741 cccaaatgcc gatctgcaca atctggccgc tagcctcggc cactgggatg aactcggccg
    10801 gtgacactga tcccaattcg ggatgatgcc agcgcagcag cgcttcggca ccgatcaccc
    10861 ggccactggc cagcgccacc tgtggctggt aatgcaggct gagttggccg cgctcgagcg
    10921 agcggcgcag tgcattctca aggtgcaggg tgcgggtcga gcgggtctgc atttcggcgg
    10981 taaaaaaacg gaagcagttg cgcccgtcct gcttggcgcg gtacatggcg acgtcagcgt
    11041 ttttagtcag ggtctcaaaa tcgctgccat cggtcgggta catggcgatg ccaatcgacg
    11101 gggtagtgag caactccatc gcgtcgatct ggtagttcat ggcaatcgcc gccagcaggc
    11161 gctcggccag atgcgcggca ccggcggcat cggtggtggg cagcagcagg ataaattcgt
    11221 cgcctccctg gcgtgacacg gtgtcttcgt cacgcaccac cgactgaatg cgccgtgcca
    11281 cctcggtcag catggcgtca ccaatgtggc ggccgagtgt gtcgttgatg ttcttgaaat
    11341 ggtcgagatc gagcgtcatc atggccagtg cgagctggct gcgctgcgcc atgctcatgg
    11401 cgtgctgaaa gcggtcaccc agcaggctta ggttggccag cccggtgagt tggtcgaaac
    11461 gcgacagcca caagatgtgt tcgctggcct gtttgctttg gctgatgtcg atgaccacac
    11521 ccagcagatt gaccatgccg ctggtcgggt cggtttcttc accgaccgac aaaatggcat
    11581 caatcacccg gccatccttg cgccgaaagc ggttttccag ggtgagccaa cggtctgggc
    11641 tttgtccgac ctcctgcatg atggtgttgc gcgtcagggg ttcctcatac aacacttcgg
    11701 caatcgagcg gcgattgacg atgtccatca gttctgcgct ggaggcgtaa ccgagaatga
    11761 ccgccatggc cggattgaca tacaaaatct tgcccgcgcg gctggtcaga aaaatgccca
    11821 ccggagcatt ctcaaagaag ctgcgcatgc gccgttcgct ctcgcgcagt gccacagcag
    11881 cctgcttgcg ctgtgcctct ttgacaaagt tgtccagcgc aaagctgatg tcgctggcca
    11941 tctccgttag aagcgtgcgc gcagcgtcgt caaaggcatg gcactcactg gcgtacaggt
    12001 taaagctgcc aatgacgaca ccctggcggt tgagcggcag tgcggcgctg ctgcgccagc
    12061 caaagtctct ggcaacttca tgccagggcg cggtcagcgg atcctgcaaa aagtcctggc
    12121 accagaccgg ctgctggcag tgcatcgccg tgccggtggg gccgcgcccc tgaggctggt
    12181 tggcatcgac cgaaatttca atgttttgca gataggcggt gccgctgcca aagctggtca
    12241 ccgggcgcac ccactggctg ccaggctcga ccagaccaat ccaggccatt ttgaaaccgc
    12301 cgaaatcgac cgcaatgcgg caagcttgtt ccagcagttc cggctcgctg ctgcagcgca
    12361 caatcgcctg gttgcactgg ctcaaggcgg cgtacagccg ctgggaatgg gccaggcgct
    12421 gctcaaggcg ttcgcgcacc cgggcatgac ggatggcgcg gcccagggcg tcggggtcaa
    12481 actggccctt gaccaggaag tcttgcgcgc cggattcaag tgtggcgagc gccagcgttt
    12541 tttcgtcgtt gccggtcagc accacgatcg gggcgctcgg caaggcggcg cgcagggttt
    12601 gcagcgtggc cagcccggcg gagtcgggca gcgacaggtc aagcagcacg atatcgggcg
    12661 ctgcctgctg cgctttggcg atgccctcgg ccagggtggt ggcccagacc ggtccgctgg
    12721 cctcgccgct ggcacagaag ccagccagac gcagataggc cttgaccagc ccaaagtcgc
    12781 cggggtcgtc ctcgatcacc aggatcgtcg gggtttcatt caaggtttgt gtcatggcgt
    12841 ttgcgtcact gtcaatttag ccgccatcgc cattgccttt gggcaggcga accaggtcga
    12901 tccagtagtc gcccaaagcc cgaatggcgg ccatgaactg cgcgacatca accggcttgg
    12961 tgatgtagcc agcagccccc agatcgtagg ccgccaccac atcgcgctcg acctcggagg
    13021 tggtcagcac caccaccgga atgtcgcgca gatcaggtaa ctgcttgatg gcggccagaa
    13081 attcgcgccc gtccaggcgc ggcatattga ggtcaagcag gatcagatcg gggcgcggcg
    13141 cgccagcata acgcgggccc aggcgctgca ggtaatcgag tgcctcgcgg ccatcaagta
    13201 catgatgcag ttcggccaga atgcggtttt cggcaatcgc caccttgacc agatgtgcgt
    13261 cggcaggctc gtcttccgcc agcaagatca caaagctgga tttttcagtt gtcatgtccg
    13321 tttctccttg gccaactcaa ttaccaggcg ggcaccgcca ccgatgcgct cttcgatcca
    13381 ggcggtgcca ccgcaactct cggcaatgcg gcgcaaaatc gccagcccga tgccggtgcc
    13441 agtgccctca ctggccggtg ccaggcgctc aaacacgcga aatatccgct cgcgatacgc
    13501 aagctcgaca ccagggccgt tgtcactgac gatgtaacac gttttatgac catcgtgcat
    13561 gccttcgatc aatataagca aaggctgcgc gctgcggcca tggttcaggg cgttgtccag
    13621 cgccacctca aacaggtcac ccagccgtgg cgggtcaatc caggcgggcg gcagttggcc
    13681 cagcgtgatc gtggcacccg cttggtcgat gcgggttttc cagcgcgcca gcaaggcgtt
    13741 cacgctcatt tgcacatcca cccagttgac cttgccgcgc ggctgatcgg ccgccagata
    13801 acgctcgaca tcgcgcagca gattctgctg gcggcgcgct tgctggccga tgtaatcaag
    13861 cgacaactga gcctcggcat catcgatccg gcccgccagt tgtttgccca ggcgctcggc
    13921 atagttggca atgcgccggg ccggctcttg caggtggtgc gcggtgactt cggcaaaacg
    13981 ttgcaggtcg gcatgggtgc gggccagcgc cgcctcaacc tccttgcgcg cagtgatgtc
    14041 acggttgctg ccgcgcagtc caagcggctg cccggccggg tcaaacaccg gctggcagac
    14101 gtgcgacacc ca
//
LOCUS       DAOSLL010000037        14054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4432_length_14054_cov_4.885420, whole genome shotgun sequence.
ACCESSION   DAOSLL010000037 DAOSLL010000000
VERSION     DAOSLL010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 14054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14054
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4432_length_14054_cov_4.885420"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="PLB25_02760"
     CDS             <1..365
                     /locus_tag="PLB25_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOE40545.1"
                     /translation="LPPEQVAQVVAIMQFEGMQATVSSIHINGWYGSHNKLEAARWMV
                     RTLFGRNLDDELERWVYVGDSTNDQLMFAHFPHSIGVANIHRFDAQLVHWPRYQTASE
                     RGAGFAEVACAILAGRAA"
     gene            379..1614
                     /locus_tag="PLB25_02765"
     CDS             379..1614
                     /locus_tag="PLB25_02765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE40546.1"
                     /translation="MTPSQRQQHELAPKAGNSQGPAAARQPGSGLGLLSVFGSTFFQL
                     CGIFMLSPLLLLMLTDAQVSSTVAGLFAATTWAGIFIITPFASQLIRYIGRRQAMWLA
                     SAAPLVAALGFMATDLLWVWFALELLAGLAGGLRWVLAEAFIAEFAPPGRIGRTIGIY
                     ATMVGLTFVVGPALLAAVGTDNRDALWVVVALLGVGLAWTALIPPLPAAHDRQSASVG
                     LPGLWHAVLAQPVIMLAGFLGGFFELGLASILPLYGLSLGLSASAATLLVAVSGAGGT
                     LLALPAGMLADRFASPAQGRRSLMAGAAVLILLATLAMPWVARANVLVWPVVAAWGAA
                     GGALYTLSMVDIGAREKGLTLINSTAVLVLTYTLGGLVASAASGALLDWSPGLGFPAL
                     LLCVATLGLATLVRQACRS"
     gene            1658..2128
                     /locus_tag="PLB25_02770"
     CDS             1658..2128
                     /locus_tag="PLB25_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HOE40547.1"
                     /translation="MFFASDLDTVTHGIQLAVAPVFLLTAVAGMIGTVAGRLARIIDR
                     ARVLEDRIDAAPADQPMLPAFNELSQLRQRGKLVNACIALLTFCAIFIGLTIVALFLG
                     ETTELQIFRVVTILFLSGVVFFLLALLGFLTETLIATRVLRFRRAKRADWADNR"
     gene            2152..4026
                     /locus_tag="PLB25_02775"
     CDS             2152..4026
                     /locus_tag="PLB25_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative nucleotidyltransferase substrate
                     binding domain-containing protein"
                     /protein_id="HOE40548.1"
                     /translation="MPNKPKKAAPLIPILASHRIWGQLSGKSLESLAAQFTLENFKAN
                     GLIRAQRQLATHLGLIISGGVQLSDPDLRLSVRLGASDMLGFGATPERQLPTWQASAI
                     GECDVAWLAPAALQQVCQMHPALAYFFPSLAPETALVNAQDSGGDVAMTLNLLSTPIR
                     SLIKRGPVTLPPDTSIQVTAALMRDLGVSSMLLADHNHLFGLVTDRDLRNRVVAEGLD
                     IRGPASDIATMAPTTVSINSPAFEALLMLARNGIEHMPVMDGRNVVGMITATDLTEQR
                     STSPVCLVGEIYQQDTLQGLVQISGRVKQLQQHLATADASAHSICHIVTAVIDALTIR
                     LIQLAEGQLGPAPINYLWVSVDSQARREQTTNSDQDNCLILSDGFDEARHGAYFSAFS
                     KFVCDGLNACGYIHCPGGMMAMTDIWRQPRKVWADYFRKWIEQPKPKALMRTCVFFDL
                     RGIHGSFELLDTLQQQVLVHTEGNSLFLAHMVNNALKHRPPLGLFGQISLIKGGANAH
                     TIDLKHSGIVPIVDLARIYTLAVGLSVPNTQDRLEVAFQAGEISQQSARDLRDALEFF
                     GKLRIAHQARQIRRGKAPDNFLALEELSNFERGHLKDAFAVIQTQQGVLHQRYLSGRS
                     "
     gene            complement(4323..4742)
                     /locus_tag="PLB25_02780"
     CDS             complement(4323..4742)
                     /locus_tag="PLB25_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40549.1"
                     /translation="MTNSTSSVAMSSKAIISGKSWRISPFIFFIGILLAVASLAHSHI
                     LTSVAQWLDQSVDRLNVAVTLIGLVSAIVDNVPLVAASMGMHSLARHPPDSFLWECMA
                     CCADTGGLITIICAAADVAAMGLEKIDFIWHAKRSPG"
     gene            complement(4904..7069)
                     /locus_tag="PLB25_02785"
     CDS             complement(4904..7069)
                     /locus_tag="PLB25_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOE40550.1"
                     /translation="MKIDRRLIQAFAIVCGATLLWLLLTMGLIAATLERSQRDLLAEH
                     LMPSLALVGLTWVLGLVPMAVALRWLFQRFATAPARLLEQTRVLLAAPHSAPLNHQGT
                     TETKALAEAIFELASQRNALRDDVAAQIAQANKSVQQEKNRLAALMSELTQSVVVCNL
                     DGRIILYNNRARLQFKTMSQAPDLAGGAELVGIGRSIYAVFERQLVAHALESIQQRSS
                     RGAGSPSAQFVTTTQAGQLLKVQMAPVRNVEASGDTAEISGFVLMLDNVTRAFEEEHL
                     RDQLLHGLTEGSRSSLASIQAAVEMLAFPDLEAPMRERFLAVVREEVSSMSQRITDMT
                     QKATEGLKARWPLEEMLGADVLLAAQRRIEVRNSRSVTLENLDTAIWLKVDSFSLTQA
                     LDYLAMRLVDEFDVKFFRLRLQASGARAQLDLIWTGQVMSTETVMSWEMDPIRFGQES
                     TPLTVRDVVERHGGEMWFERERVRHEAFFRFLLPLASVQEQLEATQILHNDSRPEYYD
                     FDLFQMSDQSSALDDRLLSELSYTVFDTETTGLNPSQGDEIIQIGAARCVNGKLLRQE
                     SFEQLVDPGRLISAASIPIHGITQDMVDGKPGITEVLPAFYAFAQDTVLVAHNAAFDM
                     KFLQLQEVHTDLVFNHPVLDTLLLSAVVHPNQESHRLEMIAERFNITVLGRHTALGDA
                     MVTAEVLLRLIPLLQAMGITTLRQAREASQKTFYARLKY"
     gene            complement(7066..7440)
                     /locus_tag="PLB25_02790"
     CDS             complement(7066..7440)
                     /locus_tag="PLB25_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE40551.1"
                     /translation="MSQKILVADDEPNIVISLEYLLKREGYAVTIARDGQEALDAIAR
                     DQPDLVLLDVMMPKKSGFEVCQELRLNEAMQHIKILMLTAKGRDTDVAKGLALGADAY
                     MTKPFATRDLVQKVAQMLGRSA"
     gene            complement(7493..10216)
                     /locus_tag="PLB25_02795"
     CDS             complement(7493..10216)
                     /locus_tag="PLB25_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOE40552.1"
                     /translation="MLQPALVIGASFAYLLLLFAVASFGDWRAAQGRSIIANPWTYAL
                     SLAVYCTAWTYFGSVGRAASGGVWFLPIYLGPTLAMMLGWQVMRKVIRIAKTYRITSI
                     ADFIGSRYGKSPLLAGLVTLIAVIGIVPYIALQLKAVSAGYALLTTSPGTGAAVSVHW
                     SQDSTFYMALALAGFTMVFGARHLDSTERHEGMVAAIAFESVIKLVAFLAVGLFVVYG
                     LFDGLADLFAKALAVPALANLLRLDQGGGFAWTQWFAMTLLSMFSVIFLPRQFQVMVV
                     ENVREVHLRRAVWVFPLYLLAINLFVLPIALGGLLYFGPGQMNADNFVLSLPLAAGQH
                     ALALLVFIGGLSAATGMVIVETIAVSTMVCNELVVPLLLRTAHFRPDAGHDLTRLLLG
                     IRRLAILGVLVLGYGYFHLAGEAYALVSIGLISFAAVAQFAPAVLGGLYWKGATRRGA
                     LAGLLAGFLMWSYTLMLPSLSKSGWLDESFLTQGLWGLAWLRPEQLFGLKGLDNLTHS
                     LFWSLLVNAGAYLGLSLWRAPSGLEASQALLFVDVFERTSSRSPVFWRGSAKVPDLLR
                     LCERFLGADKAQALFDGYARQAGVRVASAIVADAHLVQFVETQLAGAVGSASARVLVA
                     SSVEEETLTPEDVLRILDETSQLRAHSLELEEKTASLERATNELRTANEQLMSLDRLK
                     DDFMSSVTHELRTPLTSIRALAELMRDEPDMELAQRSQFMTIIVSETERLSRLVNQVL
                     DMAKIESGHAEWHAEMVDMCALVQQAVVTTAEMFRERQIVVALDLPQQAPVLLADPDR
                     LMQVMLNLLSNAAKFVPVPGGRVGVSLRRDDSGVTVAVQDNGPGVTAGQQALIFERFR
                     QGGDTSNRPQGTGLGLPISRHIVEHFGGRLWLESDPPNGAVFLFFLPITPA"
     gene            complement(10218..10430)
                     /locus_tag="PLB25_02800"
     CDS             complement(10218..10430)
                     /locus_tag="PLB25_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40553.1"
                     /translation="MFNGIDAQRLLALFGCGCLLLNFPLLGLWDVNTTVWGVPLFPAA
                     LFALWALLILALAWLMERRGQGPQDD"
     gene            10891..11199
                     /locus_tag="PLB25_02805"
     CDS             10891..11199
                     /locus_tag="PLB25_02805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HOE40554.1"
                     /translation="MQDDLVARVTSNPKYQELKSKRHTFGWTLTFLMMFAYYGFILLV
                     AFNKPLLASKIGSGVITLGMPLGIGVILFTIIITNIYVRRANSEFDDLTSQVIKAAAK
                     "
     gene            11196..12887
                     /locus_tag="PLB25_02810"
     CDS             11196..12887
                     /locus_tag="PLB25_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HOE40555.1"
                     /translation="MTKLFNPKGALALLSLLTLSAGAYAAGADLGQATKQPTNWTAIV
                     MFGAFVLATLWITKWAAAKTKSAADFYTAGGGITGFQNGLAIAGDYMSAASFLGISGL
                     VFANGFDGLIFSIGWLVGWPIITFLMAERLRNLGKFTFADVAGYRFAQTPIRVFAASG
                     SLVVVAFYMIAQMVGAGQLIKLLFGMEYLYAEILVGTIMMMYVLFGGMTATTWVQIIK
                     ACMLLGGATFMAGAVLMQFGFSPEAMFVKAIEVHSKKDAIMAPGALIKDPISAISVGM
                     ALMFGTAGLPHILMRFFTVPSAKEARKSVGWATTWIGYFYILTFIIGFGAITNLIPNP
                     TDYFVGGDITKGLNGGGNMAAVHLSKAVGGNLFMGFISAVAFATILAVVAGLTLSGAS
                     AVSHDLYASVWRKGRVDQADELRVSKITTVALGVIAVTLGIAFEKENVAYMVMLAFVI
                     AASANFPVLFMSVLWKDCTTKGAVTGGFVGLIMAVVLTIGSASVWEAVMLYPKGSAWF
                     PYNSAAIFSIPAAFITIWLVSLMDRGERAVIDRAGYPAQEVRSETGIGSSSASGH"
     gene            complement(12961..14010)
                     /locus_tag="PLB25_02815"
     CDS             complement(12961..14010)
                     /locus_tag="PLB25_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOE40556.1"
                     /translation="MITFYNQQHAQHQGKLEMFRGELVPCFEVPARVEHVLAELQRRK
                     FGPVDPPEAFDISALTGIHSPRYLDFLAHAWDEWVALDAANAVRDALPSVWPVRTLRS
                     DRLPTNFAARMGLFSMDAGTPLTSGTWEAARTGADCALSAAKALAGGARSAFALTRPP
                     GHHAGADFFGGYCFLNNAALAAQALRDQGCERVAVLDIDYHHGNGTQAIFYERADVYF
                     TSIHGDPHTEYPFYLGYADELGAGVGLGFNRNLPLPRGSGFGLWRAALAAALQDIDKF
                     AAQALVVSLGLDTFAGDPVAGFQLQSDDYLAVGADIAGAGLPTVFVFEGGYAIADMGV
                     NAVNVLQGFLQAHRA"
BASE COUNT         2785 a         4371 c         4119 g         2779 t
ORIGIN      
        1 acctgccacc cgagcaagtt gcgcaggtgg tggccatcat gcagttcgag ggcatgcaag
       61 ccaccgtcag cagcatccac atcaacggct ggtacggctc gcacaacaag ctcgaagcgg
      121 cgcgctggat ggtgcgcacc ctgtttggcc gcaacctgga cgacgaactt gagcgctggg
      181 tctatgttgg cgactctacc aacgaccaat tgatgtttgc gcatttcccc catagcattg
      241 gcgtggccaa catccaccgc tttgatgccc aactggtgca ttggccgcgc tatcaaaccg
      301 ccagcgagcg cggtgctggc tttgccgaag tggcgtgcgc cattttggcc gggcgtgccg
      361 catgatgttg gccagccgat gacacccagt caacgccagc agcacgagct tgcgcccaag
      421 gcaggcaaca gccaaggccc ggcagcagcg cgccagcccg gctcagggct gggcctgctg
      481 agcgtttttg gctcaacctt ctttcagctc tgcggcatct tcatgctgtc gccgctgctg
      541 ttgctgatgc tcaccgacgc gcaggtgtcc agcaccgtgg ccgggctgtt tgccgccacc
      601 acctgggcgg gcatcttcat catcacgcct tttgcctcgc aattgatccg ctacatcggt
      661 cggcgtcagg cgatgtggct ggcctccgcc gcgccgctgg tggccgccct cggcttcatg
      721 gcaaccgacc tgctgtgggt ctggtttgcg ctggaactgc tggccggcct ggcgggaggc
      781 ctgcgctggg tgctggccga ggccttcatc gcagaatttg ccccgccggg gcgcattggg
      841 cgcaccatcg gcatctatgc caccatggtc gggctgacct tcgtcgtcgg tcctgccctg
      901 ctggccgcag tcggcactga caaccgcgac gcgttgtggg tggtggtcgc tctgctgggg
      961 gttgggctgg cctggacagc gctgattccg ccactgccag cggcccacga caggcagagc
     1021 gccagcgtcg ggctgccggg cttgtggcac gcggtgctgg cgcagccggt gatcatgctg
     1081 gcgggttttc tgggcggttt ttttgaactg ggtctggcat ccatcctgcc gctgtacggg
     1141 ctgtcgctgg gcctgagcgc cagtgccgcc accttgctgg tggcggtaag cggtgcgggt
     1201 ggcaccctgc tggccttgcc ggcgggcatg ctggccgacc gttttgcctc gccggcgcaa
     1261 ggtcggcgca gcctgatggc gggtgccgcc gtgttgatct tgcttgctac cctggccatg
     1321 ccgtgggtgg cgcgggccaa tgtgctggtc tggcccgtgg tggcagcatg gggtgcggca
     1381 ggcggcgcgc tctacacctt gtcgatggtg gatattggtg cgcgcgagaa agggctgacg
     1441 ctgatcaaca gcaccgccgt gctggtgctc acctacaccc tgggcggact ggtggcctct
     1501 gccgcatctg gcgctttact tgactggtcg cccggccttg ggtttccagc cctgctgctt
     1561 tgtgtggcaa cgcttggcct ggcgacactg gtcagacaag cctgccgatc ttgaaagtcg
     1621 ctgctaaagt gaccccgatt accaacggag gtaccccatg ttttttgcat ctgacctcga
     1681 taccgtcacc catggcatcc agctcgccgt ggccccggtt tttttgctga ctgccgtagc
     1741 cggcatgatt ggtacggtgg ctggtcggct ggcgcgcatc atcgaccgcg cccgtgtgct
     1801 ggaagatcga atagatgcgg caccggcaga ccagccaatg ctgcctgcct tcaacgaact
     1861 cagccagttg cgccagcgcg gaaagttggt caacgcctgc attgcgctgt tgaccttctg
     1921 cgccattttc attggtttga cgatagtggc gctgttcctt ggcgagacca ctgaattgca
     1981 gatatttcgg gtggtgacca tcctgtttct gtccggtgtg gtgtttttct tgctggcact
     2041 gctgggtttt ttgaccgaaa cgctgattgc aacccgcgta ctgaggttta ggcgagcaaa
     2101 gcgcgcagac tgggcagaca atcgctgatc cattttgggt gtcactccat catgccgaac
     2161 aagccgaaaa aagctgcgcc tctgatcccg atccttgcca gccaccgcat ctgggggcag
     2221 cttagtggca aaagcctgga aagcctggcg gcacagttca cccttgaaaa tttcaaggcc
     2281 aatggcttga ttcgggcaca gaggcaactg gcgacccatc tgggcctgat catcagcggc
     2341 ggggttcagc tcagcgaccc cgatctgcgc ctttcagtcc gcctcggggc ctctgacatg
     2401 ctgggctttg gtgccacgcc agagcgccag ttgccaacct ggcaggcgag cgccatcggc
     2461 gagtgcgatg tcgcctggct ggcaccggcg gccttgcagc aagtgtgtca aatgcacccg
     2521 gcgctggctt atttttttcc ttcgctggcg cctgagaccg ccttggtcaa tgctcaagac
     2581 agtggcggcg atgtggcaat gaccctgaat ttgctcagca caccgatcag atcactgatc
     2641 aagcgcgggc cggtgacgct gccgcccgac accagcattc aagtcaccgc cgccctgatg
     2701 cgtgatctgg gggtttcctc gatgttgctg gcggatcaca accatctgtt tggcctggtc
     2761 accgaccgcg atctgcgcaa ccgggtggtg gcagaaggcc tggacattcg tggtccggcc
     2821 tctgacatag ccaccatggc accgaccacc gtcagcatca acagtccggc ttttgaagcc
     2881 ctgctgatgc tggcacgcaa cggcattgag cacatgccgg tgatggacgg ccgcaatgtt
     2941 gtcggcatga tcaccgctac cgatctgacc gagcagcgca gtacctcgcc ggtgtgtctg
     3001 gtcggcgaaa tctaccagca agacacgctc caaggcctgg tccagatcag cggccgggtc
     3061 aagcaactgc agcaacacct ggccaccgct gatgccagcg cccacagcat ctgccacatc
     3121 gtcaccgctg tgatcgacgc gctgaccatc cggctgattc agctcgctga aggccaactc
     3181 ggcccggccc ccatcaacta tctgtgggtg tcggtcgact cgcaggcccg ccgcgagcaa
     3241 actaccaatt cagaccagga caactgcctg attctgagcg acggctttga cgaggcccgg
     3301 cacggggcct acttcagcgc gttctccaag tttgtctgtg atggtctgaa tgcctgcggt
     3361 tacattcatt gcccaggcgg catgatggcc atgaccgaca tctggcgcca gccgcgcaaa
     3421 gtctgggccg actactttcg caagtggatc gaacagccca aacccaaggc cttgatgcgg
     3481 acctgcgtgt ttttcgacct gcgcggcatt cacggcagct ttgaattgct cgacactctg
     3541 cagcagcagg tgctggtgca caccgagggc aatagcctgt ttctggcgca catggtgaac
     3601 aacgccctca agcaccgccc gccgctgggc ctgttcgggc aaatatcgct catcaaaggc
     3661 ggtgccaacg cccacaccat cgacctcaag cacagcggca tcgtgccgat cgtggacctg
     3721 gcgcggattt acaccctggc ggtcggcctg agcgtgccca acacccagga ccggcttgaa
     3781 gtggcattcc aggctggcga gatcagccaa caaagcgcgc gcgacctgcg tgacgcactg
     3841 gagtttttcg gcaagttgcg aattgcccat caggcccgtc agattcgccg tggcaaagcg
     3901 ccagacaact ttctggccct tgaggaactg tccaattttg agcgcggtca cctgaaagat
     3961 gcctttgccg tgatccagac ccagcaaggg gtgctgcatc agcgctattt gagtggccgg
     4021 tcttgatccg cccgggcggt ggccgtgctt ttttacaagc gggcaaccga gcaatcctgg
     4081 attgatgatc cgggtctgct gattaaagcg ctgcaggctg acgaaagcgc aggccagatt
     4141 tcgctaacca accgattcgc ctgcgcaggc aaggttttat gataaaaaag gcctgaagcc
     4201 cttatagaac gtgcgctaat agctattgtt taaatagcat ttcgccaata tctctgatcc
     4261 ttcagtgggt catctggaac tcaatcacgc acgccactgc cccgtcaaaa taaccagtgc
     4321 agtcagccag gtgatctttt tgcatgccag atgaaatcga tcttctccag tcccatggca
     4381 gccacatcag cggcggcgca aatgatcgtg atcaagcccc cggtgtcggc acagcaagcc
     4441 atgcattccc acaagaagct gtctggcggg tgccgggcca ggctgtgcat gcccatcgac
     4501 gctgccacca agggcacgtt gtccacaatc gcgctgacca gaccgatcaa ggtcacggcg
     4561 acattcaggc ggtccacgct ttgatccagc cattgcgcca ccgaagtcag gatgtgactg
     4621 tgcgccagcg aggccaccgc cagcaaaatg ccgatgaaga agatgaacgg actgattcgc
     4681 cagctttttc cggagatgat ggccttcgaa ctcatcgcca ccgaggatgt cgaattggtc
     4741 atgcatcgac tcaaccaccg gcccagaaat tgcctcggct acagagcttc aattgatgtt
     4801 tttatggagc agctacaact gcataatcca tcggcggcac gtcagactgg aatccgcccc
     4861 caggagaagc caatgccaaa gcaaccgaca gcagccaaag gcttcagtat ttgagccgtg
     4921 cgtaaaaggt cttttgcgat gcctcgcgcg cctggcgcag ggttgtgatg cccatcgcct
     4981 gcaacagcgg aatcagccgc agcagcacct cggccgtcac cattgcatct cccagcgcgg
     5041 tgtggcgccc cagcacagtg atgttgaaac gctcggcaat catctccagc cggtgcgatt
     5101 cctggttcgg gtgcaccact gcagacagca gcagggtgtc gagcaccggg tggttgaaca
     5161 ccaggtcggt gtgcacctct tgcagttgca aaaacttcat gtcaaatgcc gcgttgtgcg
     5221 ccaccagcac ggtgtcttgc gcaaaggcgt aaaaggccgg cagcacctcg gtgatgccgg
     5281 gcttgccatc caccatgtcc tgggtgatgc catgaatcgg aatggaggcc gccgaaatca
     5341 ggcggccagg gtcgaccaac tgctcaaagc tctcttgccg cagcaacttg ccgttgacgc
     5401 agcgcgccgc accgatctgg atgatctcgt cgccttgaga ggggttcagg ccggtcgtct
     5461 cggtgtcaaa cacggtgtag gacagctcgc tgagcagccg gtcatccagc gcgctgctct
     5521 gatcggacat ctggaacagg tcaaagtcgt aatactctgg gcggctgtcg ttgtgcaaaa
     5581 tttgcgttgc ctccagttgc tcctgcacac tggccagtgg cagcaagaat ctgaaaaacg
     5641 cctcgtgtcg aacccgctcg cgttcgaacc acatctcgcc gccgtggcgc tcaaccacat
     5701 cgcgcaccgt cagcggggtg ctttcctggc caaagcggat cgggtccatc tcccagctca
     5761 tgacggtttc agtgctcatc acctggccgg tccagatcag gtcaagctgc gcgcgtgcgc
     5821 cgctggcctg caggcgcagc cgaaagaact tgacatcaaa ctcgtcgacc aggcgcatgg
     5881 ccagatagtc cagcgcctgg gtcaggctga aactgtccac cttgagccag atcgcggtat
     5941 ccagattttc cagcgtcacg ctgcggctgt tgcgcacctc gatgcggcgc tgtgctgcca
     6001 gcaacacatc ggcacccagc atttcttcca gcggccagcg cgccttgagt ccttcggtgg
     6061 ccttttgtgt catgtcggtg atgcgttggc tcatgctgct gacctcttca cgcacgacgg
     6121 ccaaaaagcg ctcgcgcatg ggtgcctcca ggtctggaaa agccagcatc tcgaccgccg
     6181 cctgaatact ggccagcgac gaacggctgc cctcagtcag gccgtgcagc aactggtcgc
     6241 gcaggtgttc ttcttcaaag gcccgggtga cgttgtcaag catcagcaca aagccgctga
     6301 tctcggcagt gtcaccggat gcctcgacgt tgcgcaccgg ggccatttgc accttgagca
     6361 gttgcccggc ctgggtggtg gtgacaaatt gggccgacgg gctgccggca ccccggctac
     6421 tgcgctgctg aatgctctcc agtgcgtggg ccaccagttg ccgctcaaaa accgcgtaaa
     6481 tcgagcggcc aatgccgacc agctcggcac caccagccag atccggcgct tgcgacatgg
     6541 ttttgaattg cagccgggcg cggttgttgt acagaatgat gcggccatcc agattgcaca
     6601 ccaccacgct ttgggttagt tcggacatca gcgcggcaag gcggtttttt tcctgctgca
     6661 cactcttgtt ggcctgggca atctgcgctg ccacgtcgtc gcgcagggca ttgcgctggc
     6721 tggccagctc aaaaatcgcc tccgccagtg ctttggtctc ggtggtgccc tggtggttca
     6781 gcggtgcgga atgaggcgcg gccagcagca ccctggtttg ctccagcaag cgcgccggag
     6841 cagtggcaaa gcgctggaac agccagcgca acgcgactgc catcggaacc aggcccagca
     6901 cccaagtcag gccaaccagg gccagactcg gcatcaaatg ctcagccagc agatcacgct
     6961 ggctacgttc cagagtcgcg gcaatcaagc ccatcgtcag cagcagccac agcagggtgg
     7021 caccacagac gatagcaaaa gcctgaatca ggcgacgatc aatcttcatg cgctgcggcc
     7081 caacatttgt gccactttct gcaccagatc gcgggtggcg aaaggcttgg tcatgtaggc
     7141 atcggctccc agcgccaggc ccttggcgac atcggtgtcg cgccccttgg cggtgagcat
     7201 caggatcttg atgtgttgca tggcctcgtt gaggcgtagc tcctggcaga cttcaaagcc
     7261 ggattttttc ggcatcatca catcaagcag caccaggtcc ggctgatccc gcgcaatggc
     7321 gtcaagcgcc tcctggccgt cccgtgcaat ggtcactgcg tagccctcgc gcttgagcag
     7381 atactccagg gaaatcacga tattgggctc gtcgtcggcc accagtattt tttggctcat
     7441 gtctgtctcc gtgtctctct ttgtatatat atatatggtg tcatggatga ttctaggccg
     7501 gggtgatggg caggaaaaac aggaaaaccg ccccattggg gggatctgat tcgagccaaa
     7561 gccgcccgcc aaaatgctcc acgatgtgtc ggctgatcgg caggcccagg ccggttccct
     7621 gcggtcggtt gctggtgtcg ccgccctggc gaaaacgctc aaaaatcaag gcctgctgcc
     7681 cagccgtcac gccgggtccg ttgtcttgca cggccaccgt caccccgcta tcgtcacggc
     7741 gcaggcttac accaacccga ccacctggca ccggcacaaa cttggcggca ttggacagca
     7801 ggttgagcat cacctgcatc agccggtcag ggtcggccag caacaccggt gcctgctgtg
     7861 gcaagtccag cgcgacgaca atctgacgtt cccggaacat ctctgccgtg gtcaccacgg
     7921 cctgctgcac cagcgcgcac atgtcaacca tttcggcatg ccactcggca tggccagatt
     7981 cgatcttggc catgtcgagc acctgattca ccagccgtga cagccgctcg gtctcgctga
     8041 cgatgatggt catgaactgg ctgcgctggg ccagctccat gtcgggttca tcgcgcatca
     8101 gctcggccag cgcccggatc gaggtcagcg gcgtgcgcag ttcgtgggtc accgacgaca
     8161 tgaagtcgtc tttcaaccgg tccaggctca tgagctgctc attggcggtg cgcagctcat
     8221 tggtggcgcg ttccagcgag gcggtttttt cctcgagttc gagcgaatgc gcgcgcagtt
     8281 gcgaggtttc gtccaggatt cgcagcacat cctcgggggt cagggtttcc tcttcaacgc
     8341 tggaggccac cagcacccgg gccgaggcgc tgccgaccgc cccggcgagc tgggtttcga
     8401 caaactgcac cagatgggca tcggccacaa tggccgacgc aacccgcacg cccgcttgcc
     8461 gggcgtagcc gtcaaacaag gcctgcgcct tgtccgcccc cagaaaacgc tcgcacaggc
     8521 gcagcaaatc aggcactttg gcgctgccgc gccagaacac cgggctgcgg ctgctggtgc
     8581 gctcgaacac atcgacaaac agcagcgcct ggctggcctc caggccagaa ggggcgcgcc
     8641 atagcgacaa gccaaggtag gcaccggcat tgacaagcaa gctccaaaac agcgagtggg
     8701 tcaggttgtc aagccccttc aggccaaaca actgctcggg ccgcaaccac gccaggcccc
     8761 acaggccctg ggtcaaaaat gactcatcga gccagcccga cttggacagc gagggcagca
     8821 tcaaggtata ggaccacatc aggaacccgg ccagcaagcc cgccagcgcg ccccggcggg
     8881 tggcaccttt ccagtacagg ccgcccagca cggccggggc aaactgcgcc actgcggcaa
     8941 agctgatcag cccgatgctg accagcgcgt aagcctcgcc ggccaggtga aagtagccgt
     9001 agcccagcac cagcacaccc aaaatggcca gccgccgaat acccagcagc aggcgggtca
     9061 ggtcgtgccc ggcatcgggg cgaaaatgcg cggtgcgcag cagcagcggc accaccagct
     9121 cgttgcacac catggttgac accgcaatgg tttccacaat caccatgccg gtggccgccg
     9181 acagcccgcc aatgaacacc agcaaggcca gcgcatgctg cccggcggcc agtggcagcg
     9241 acagcacaaa gttgtccgcg ttcatctggc ccggcccaaa atacagcaag ccgcccaggg
     9301 caatcggcag cacaaacagg ttgattgcca gcaggtacag cggaaacacc cagacggcgc
     9361 ggcgcaaatg gacctcgcgc acgttttcca ccaccatcac ctgaaactgg cgcggcaaaa
     9421 aaatcaccga gaacatcgac agcagggtca tcgcaaacca ctgggtccag gcaaagccac
     9481 cgccctggtc gagccgcagc aaattggcca gcgcgggcac tgccagcgct ttcgcaaaca
     9541 ggtcggccag gccatcaaac aggccgtaca ccacaaacag cccaaccgcc aggaaagcca
     9601 ccagcttgat gaccgactca aaggcaattg ccgccaccat gccttcgtgc cgttcggtcg
     9661 agtccagatg gcgggcacca aacaccatgg taaagccggc cagcgccagc gccatgtaaa
     9721 acgtgctgtc ctgactccag tgcaccgata ctgccgcgcc ggtgccgggt gatgtggtca
     9781 gcaaggcata gccggccgat accgccttga gctgcaaggc aatgtaggga acgatgccga
     9841 tcaccgcaat cagcgtcacc agaccggcca gcagcgggct tttgccatag cggctgccaa
     9901 tgaagtcggc aatcgaggtg atgcggtagg tcttggcaat gcggatcacc ttgcgcatca
     9961 cctgccagcc cagcatcatg gccagggtcg gcccgagata aatcggcaaa aaccagaccc
    10021 cgcccgaagc cgcccgcccg acgctgccaa agtaggtcca ggcggtgcaa tacaccgcca
    10081 gcgacaaggc ataggtccag gggttggcga tgatggagcg cccctgcgcg gcccgccagt
    10141 cgccaaaact ggccaccgcg aacagcagca gcaggtaggc aaacgaagcc ccaatgacca
    10201 gtgcaggctg cagcatgtca gtcgtcctgt gggccctggc cgcgtcgctc catcagccag
    10261 gccagggcca gaatcagcag cgcccacagc gcaaacaacg cggccggaaa cagcggcacg
    10321 ccccaaaccg tggtgttgac atcccacagg cccaacagcg gaaaattcag cagcagacag
    10381 ccgcagccaa acagggccag caggcgctgt gcatcgattc cgttgaacac gtctttgtct
    10441 cctgtaatgg gctgaaactg gggctcgcca aaactcgatg gctcacctgt tgccgcatgg
    10501 attattgatt gtcttgtcgc atgaatccac cactgaaagc ccgcatgccc gaattggggc
    10561 aaaaacctag ggaaaacacc gagtcaaaag actttgcccg gcaaaacagg ccgagcttgt
    10621 tgtccgcagg tcgtcattct caccaatgaa aatggccatt aggccaatag caatatgatt
    10681 agtttgctat tgtttgtata tgcctgcaaa ggaagctcaa tgcaccagga atgcgcgatc
    10741 agcccaattc cccttggtgc tctgcgggat tggcatccaa acgactgcac ctgttgacga
    10801 aactgtcgtt gactggtttt tgcggtcagc aattggtaag aactgtcatt tagcctcgcg
    10861 ccggtcacat ttttaatagg agacactttt atgcaggacg atttggttgc aagggttacc
    10921 agtaacccca agtaccagga gctcaagtcc aagcggcaca ccttcgggtg gacgctaacg
    10981 ttcttgatga tgtttgccta ctacggcttc attttgctgg tggccttcaa taagccgctg
    11041 ttggccagca agattggctc gggtgtgatc acgctcggca tgccgctggg cattggtgtg
    11101 attctgttca ccatcatcat caccaacatt tatgtccgtc gggccaacag cgagttcgac
    11161 gacctgacat cacaagtcat caaggcggca gcgaaatgac caaattattt aaccccaagg
    11221 gcgcgcttgc gctgctgtct ttgctgaccc tgtctgccgg tgcttacgcg gccggtgctg
    11281 acctggggca ggccaccaag caacccacca actggaccgc catcgtcatg tttggcgcat
    11341 ttgtgctggc cacgctgtgg atcaccaagt gggcggctgc caaaaccaaa agtgcggcag
    11401 acttctatac cgctggtggt ggcattaccg gctttcagaa tggcctggca attgcgggcg
    11461 actacatgtc ggcagcctcg tttctgggca tttcagggct ggtgttcgcc aacggctttg
    11521 acggcctgat cttctcgatc ggctggctgg tcggctggcc aatcatcacc ttcctgatgg
    11581 ccgagcgcct gcgcaacctg ggcaagttca cctttgccga cgtggccggt taccgttttg
    11641 cccagacacc gattcgcgtg tttgcggcca gcggttcgct ggtggtggtg gctttctaca
    11701 tgattgccca gatggtcggt gccggccagc tcatcaagct gctgtttggc atggagtacc
    11761 tatacgctga aatcctggtg ggtaccatca tgatgatgta tgtgctgttt ggcggcatga
    11821 cggctaccac ctgggtacag atcatcaagg cctgcatgtt gctcggcggt gccaccttca
    11881 tggctggcgc ggtgctgatg cagttcggtt tttcgcctga ggcgatgttt gtcaaggcga
    11941 ttgaagtgca ctccaagaag gatgccatca tggctcctgg cgcgctgatc aaggatccga
    12001 tctctgccat ttcagttggc atggcactga tgtttggtac tgctggtctg ccgcacatcc
    12061 tgatgcgctt ctttaccgtg cctagtgcca aggaagcccg caagtcggtc ggctgggcca
    12121 ccacctggat tggctacttc tacatcctga ccttcatcat cggttttggt gccattacca
    12181 acctgattcc caacccgaca gactactttg tgggtggcga catcaccaag ggcttgaacg
    12241 gcggcggcaa catggcggcg gtgcatttgt ccaaggcagt cgggggcaat ctgttcatgg
    12301 gctttatctc ggccgtggca tttgccacca ttttggcggt ggtagctggc ttgacgctgt
    12361 cgggtgcctc ggccgtgtca catgacttgt acgcctccgt atggcgcaag ggccgggttg
    12421 accaggctga tgagttgcgc gtatccaaga tcaccaccgt ggctttgggt gtcattgccg
    12481 tcactttggg cattgctttc gagaaagaaa acgtcgccta catggtgatg ctggctttcg
    12541 tgattgccgc ctcggccaac ttcccggtgc tgttcatgtc ggtgctgtgg aaagactgca
    12601 ccaccaaggg tgctgtgacc ggtggctttg ttggcctgat catggccgtt gtgctgacca
    12661 ttggttcagc cagcgtgtgg gaagccgtga tgctgtatcc caagggctcg gcatggttcc
    12721 cgtacaactc ggcagccatc ttctcgattc cagccgcctt catcaccatc tggctggtgt
    12781 cgctgatgga ccgtggcgag cgtgccgtga ttgaccgcgc tggctacccg gcgcaagaag
    12841 tacgttcaga aaccggcatt ggctcctcgt cagcctccgg ccactaagcg gcttcacaac
    12901 cccggcgttg ccggggttgt accgtcaagg actgtcagcg catcgctgcc agtccttttt
    12961 ttatgcccga tgcgcctgca gaaagccttg cagcacattg actgcgttga cccccatgtc
    13021 ggctatggca taaccgccct caaacacaaa cacggtgggc aagccagccc ctgcgatatc
    13081 tgcaccaacg gccagatagt cgtcactttg cagttgaaaa ccggccaccg gatcgccagc
    13141 aaaggtgtcc agacccaagg acaccaccag cgcttgcgca gcaaatttgt cgatatcttg
    13201 caaggccgca gccaacgcgg cgcgccatag accaaatccg ctgccacgag gcaagggcag
    13261 gttgcgattg aaacccaacc ccactcccgc acccaactca tcggcgtaac ccagataaaa
    13321 cgggtattcg gtgtgcggat cgccatggat gctggtgaaa taaacgtcag ctcgttcgta
    13381 aaaaatggcc tgcgtgccat tgccgtggtg atagtcaatg tccagcaccg cgacccgctc
    13441 gcagccctgg tcgcgcaaag cctgcgcggc caaagcggca ttgttcaaaa agcaatagcc
    13501 accaaaaaag tcagccccgg catggtgccc aggcggacga gtcagggcaa aggccgagcg
    13561 cgcaccgcct gccagcgctt tggcagcgct cagcgcgcaa tcggctccgg tgcgcgctgc
    13621 ctcccaggtg ccagaggtca ggggcgtgcc tgcatccatc gaaaacaaac ccatgcgcgc
    13681 ggcaaagttg gtcggcagcc gatcagatcg cagtgtgcgc accggccata ccgagggcag
    13741 tgcgtcgcgc accgcgttag ccgcatccag cgccacccac tcatcccagg cgtgcgccag
    13801 aaaatcgagg taacgcgggc tgtggatgcc ggtcaatgcg ctgatgtcaa aggcctcggg
    13861 tggatcgact ggaccgaact tgcgccgctg caactcagcc agcacatgtt caacccgcgc
    13921 aggcacctca aagcacggca caagttcgcc acgaaacatc tcaagcttgc cctggtgctg
    13981 ggcgtgctgc tggttgtaaa aggtgatcat gctctatttt cttgggtaaa ttggcctcta
    14041 gcccttgata aaca
//
LOCUS       DAOSLL010000038        14028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4444_length_14028_cov_5.292779, whole genome shotgun sequence.
ACCESSION   DAOSLL010000038 DAOSLL010000000
VERSION     DAOSLL010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 14028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14028
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4444_length_14028_cov_5.292779"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..802
                     /gene="fabR"
                     /locus_tag="PLB25_02820"
     CDS             119..802
                     /gene="fabR"
                     /locus_tag="PLB25_02820"
                     /inference="COORDINATES: protein motif:HMM:NF008402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HOE40557.1"
                     /translation="MPISQPVGQPALTRSERKDITRASLVQAALALIGKGRSFTSLGI
                     REIAREADLVPNAFYRHFRNVDELGLALVEQLGITLRRLLREARQTGVAPNAMVHRSV
                     QVYLEYVRQNRLQFLFISSERSGGSRSLRLAIRQDVNQFTNEMAQDFRRLGMFPDMPT
                     STLQMVCGLIVTTMLAAAPEILDLPPGQPLLEAQMTENFVLQLQLVLLGASCWKDPAA
                     RKISSQISL"
     gene            complement(1009..1803)
                     /locus_tag="PLB25_02825"
     CDS             complement(1009..1803)
                     /locus_tag="PLB25_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40558.1"
                     /translation="MTNPSQFTQGRQVSSPAAADMLTTAITAHGGAERWQQVAVIEAA
                     LSSYGLAFSMKQQGSALKNLRISVYPHQQRVTLGGFAKPEWCGIWTPQQVLLQDGDGK
                     LVAQRDNPYAAFFTLKKRLAWDKLDILYFAGKALWNYLCFPFYLANEGVTLTGIDAGG
                     ESGSRMLRAEFKPAVPSHSVLETFHIDDAGLLLRHDYTATVIGSWATAANRCLNWERV
                     DGLRFYTRRRVTPRFGDQLVLPAPMLVWIELTDIRVINKPEEHAAR"
     gene            complement(2125..3987)
                     /locus_tag="PLB25_02830"
     CDS             complement(2125..3987)
                     /locus_tag="PLB25_02830"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE40559.1"
                     /translation="MNLNAKVTLFFVSIAAGLLVVLIAISVYALRSFSITSATEHIRT
                     AGEIVRVHLTESMINGVIDKRERFLLRLVEIQGLKSARVIRSPEVAKQFGQGLAVESI
                     ADEIELAVMREGKARFELLSRSDETVFRGTIPYIATATGTPNCLQCHQVPEGTVLGAV
                     SMSMSIDALKEKALFTIASIAGAVIVFSLLMILLLRRLLRPISDTANAVEDAVQRALL
                     GDFQAQVEKRTSDEIGQIATDMNRLLGFLDNGLNRIGSHVARLTERTPAPGENLLTAT
                     IDMVEGLTKAAHFKQAIEEDESKTEIYRRLANTLKNEFRLTEFSIYEVSGNKKQMKPI
                     LVDGEAADSCRWCNPQILVRPEACRVRRTGHLVNGIVHADICNSFMPPAELGDRRHFC
                     FPVIQSGAVGSVVQLLAGPADQQTLQDKTPFINVYLREAAPVLETKRLMESLRDSTLQ
                     DPMTGLHNRRFLEEYVDTLVANVQRKSAHVAILMLDLDYFKMVNDTYGHDAGDAVLKA
                     LSTVLKQSVRASDLVIRYGGEEFLIILVDSAGEAADRVAESIRLAVEGLKVKVGTIEL
                     CKTISIGISDFPANSETFWQAVKFADIALYQAKEQGRNRVIRFKPSMWADTKDY"
     gene            complement(4176..6695)
                     /locus_tag="PLB25_02835"
     CDS             complement(4176..6695)
                     /locus_tag="PLB25_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HOE40560.1"
                     /translation="MTISFFKLGWRSLTRDLRAGELRLLMVAVTLAVAALTAVGFFAD
                     RLKSGLQRDARQLLGGDAVVVSDNPTPAAFLAQARTLGLQQVQTLTFPTMVRAPDAQG
                     GQSNLVALKAVQAGYPLRGNLRISSTPGSAGALTRAVPAPGQVWIEPALLEALNLRLG
                     DTLLLGDSSLQVAQLIVTEPDRGAGFMNFAPRVMVNLADLAATGLVQPASRLSYRMAV
                     AGEEQSVQAFVDWSQAQVQARAARGLRVEALQGGRPELSTTMERAEKFLNLVALLAAL
                     LSAVAVALAARGFAANHLDDCAMLRVLGLPQRTIAAAYSFEFVLIGLLASAVGVALGF
                     AVHYGFIALLAGLVDAGLPTPGIWPVLFGLGMGLTLLLAFGLPPVLQLAQVPPLRVIR
                     RDVGSLKPISLLVLGLGVAGFAALLMAASSDVKLGLIAVGGFAGATLLFAALSWLAVK
                     LLRRFVNDTTAPTALVLATRQISARPAFAVVQVSSLAVGLLALVLLVLLRTDLINSWR
                     QASPADAPNRFVINIMADQAGAFSQTLKDAGVLQFDWFPMIRGRLVAINDRDVSPDDF
                     IDERARRLIDREFNLSHSAALPKQNLVVAGRWQDDEAGAISIEEGIAKTLGLSIGDRL
                     RFDVGGVPTDSRITSLRKVDWGSMRANFYAMYPVGKLDDLPATYMSAFKAPVTAGFDN
                     ALVRQFPNITSVDMTATIAQIQRVLDQVVGAVEFLFAFTLAAGLVVLFAAVTATREER
                     AREFAIMRAVGARASLLRQVQRIELIGVGLLAGLLASLVASVVGWALARYVFEFTWVV
                     SLWVPLLGALAGAVLALAAGWWGLRDVLRRPVVQTLRRAAQ"
     gene            7268..7993
                     /locus_tag="PLB25_02840"
     CDS             7268..7993
                     /locus_tag="PLB25_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40561.1"
                     /translation="MLNPSQQALKAVSLALLLCLGPLAQAADAVTQAMQTANGPYRMA
                     LFKTNGTSQAESQQALTQAMQAWDKLSSEFAAKPAAPYDRDPAFASSVAAVSKVYAKA
                     QGEISANDLKAAHSTLEEAREIMSAMRKRNNVVVYSDHMNAYHEVMEHILVDGNALLA
                     QPKGLLVMTAQAGALSYVAKRLSTQAPESLAKDAEFQALVDAVKQSVDRLESALQNQD
                     QAAVKDAMGKLKGPYSKLFAKFG"
     gene            8017..8364
                     /locus_tag="PLB25_02845"
     CDS             8017..8364
                     /locus_tag="PLB25_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOE40562.1"
                     /translation="MSTFDAIGLIKGINENLGPFRKSQSEAMLGFGQLAKASMAEGAV
                     SAKNKELIALAIGITQHCHGCIAFHVKALRRLGCSRAELEETLAVCVYMGGGPGLMTA
                     ADALSAWDEMESA"
     gene            complement(8492..8941)
                     /gene="nrdR"
                     /locus_tag="PLB25_02850"
     CDS             complement(8492..8941)
                     /gene="nrdR"
                     /locus_tag="PLB25_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HOE40563.1"
                     /translation="MKCPFCSHPETQVAETRVSEDGGFIRRRRRCMSCEKRFTTYERP
                     EVTFPAIVKKDGRRIEYDHNKLLASMKLALRKRPVSTELVDSAAERIEEQLLSLGLRE
                     VLSARIGELVMLELKKLDKVAYVRFASVYRSFEDIDEFKALVDEVRR"
     gene            complement(9039..10286)
                     /gene="glyA"
                     /locus_tag="PLB25_02855"
     CDS             complement(9039..10286)
                     /gene="glyA"
                     /locus_tag="PLB25_02855"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HOE40564.1"
                     /translation="MFNRNILIEQTDPELFSAIQAENARQEHHIELIASENYASPAVM
                     AAQGTQLTNKYAEGYPGKRYYGGCEFVDIAEQLAIDRVKQIFGAEAANVQAHCGASAN
                     EAVFLAFLKPGDTIMGMSLAEGGHLTHGMALNMSGKWFNVVSYGLDASEAIDYDAMER
                     KAHEHKPKLIIAGASAYSLAIDFARFAKVAKDVGAIFMVDMAHYAGLIAAGIYPNPVP
                     HADVVTSTTHKSLRGPRGGIILMKAQHEKVINSAIFPGLQGGPLMHVIAAKAVAFKEA
                     LQPEFKLYQQQVLVNARIVAETLTERGLRIVSGRTESHVMLVDLRAKGITGKEAEAVL
                     GSAHMTINKNAIPNDPEKPMVTSGVRIGTPAMTTRGFKDEEARITAHLVADVLDNPRD
                     SSNIDAVRAMVNALTARFPVYSA"
     regulatory      complement(10333..10464)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01750"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ZMP/ZTP riboswitches appear to regulate genes
                     involved in one-carbon metabolism; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="5-aminoimidazole-4-carboxamide
                     ribonucleotide (ZMP)/
                     5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl
                     5'-triphosphate (ZMT)"
                     /db_xref="RFAM:RF01750"
     gene            complement(10482..11312)
                     /locus_tag="PLB25_02860"
     CDS             complement(10482..11312)
                     /locus_tag="PLB25_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOE40565.1"
                     /translation="MSATHTMTIRLRRLTTVVTKGFFEVTHNSFALIGLAVLFATMAL
                     LARPELRELGEVKLFGWLQERQVIVTGLVSDPVASERATATDPADLPKQQAAVAFWLS
                     KKYNVAPEPISALVSEAYEIGSQTRLEPTLILAVMAIESGFNPFAQSPVGAQGLMQVM
                     TRVHSDKYQSFGGKFAAFDPFANLHVGVKVLQDCIRVAGSVEGGLKYYVGAANVELDG
                     GYAAKVMAEHAKLQQVALGRKPPTTSNPSAPVIRALPPAAADAAPEAEPDKVATVALQ
                     "
     gene            11616..>14028
                     /locus_tag="PLB25_02865"
     CDS             11616..>14028
                     /locus_tag="PLB25_02865"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HOE40566.1"
                     /translation="MNFAIFSRLSPSRQQWLHRAGAFVLGLLLLWLLAWLAVPPILKS
                     QVQRLLGEQLGRAVSIGQIDFKPWTLELDVQDLAVAHAATATDRPASAGEPVVKPQVH
                     IKRLYFDLELQSLLRLAPVVDALTIESPALQLRHLGQGRYDVDDVLTRLASRPTNPDA
                     APLRFALYNLVLQDGGLDFTDDAQGKVHRLSALKISLPFLSNLDALRDVQVQPLLAFD
                     LNGSRFDSTAHTTPFAQTRKTEAQFSLKRLDLAPYLGYWPASLPARLTSAVIDADLKL
                     VFEQAQQPHVAISGLLSASGVKLQATGKGTAALAVPELLAFDKLAVQFKSLRPLERVA
                     QLSRVDWQQPLLTLHRNKQGQVNWLALAKAQQTPSNASKTVASSADRARATGQKQAET
                     RADARPAGWQLGIDQIALEGGEIRWLDDAAAAPVRLALTGLTLQAQALAWPFTQPMPF
                     QGAGRLNSATLTFKGDATDQAADVSVSLADLPLSLAAPYLASQFIPTLDGMLTTELAL
                     NWKAASATGAGAQILLKVPSLTLDKLLLSDPRKARLASIKQVRLETAELDVARQTVTL
                     ARLRLDQPQGHLARDAQGRWNFEDWRKDQLAVQAAAPSAQPATGTTRTTTKTTAAAAA
                     PLPAAVAPVSAGAPADAARPWLVTVQDFALSDASLGFQDMAHGKAVALDLSALSLQLK
                     NFSSAAGKPFGLTLAARVRHGNTEPGQLSWRGTGSLSPLSLQGDVLAQRLPAHALAAY
                     FGDALNVAPLRLDASFKGKLDLAQQSAGLALQVRGDARLEDVLVHSLAATQGATATPA
                     ANAPAP"
BASE COUNT         2911 a         4235 c         4167 g         2715 t
ORIGIN      
        1 gcatcccaac tccttggtgc aaaacgggga gttattcccg accacatcct aagtccatgt
       61 tttcattgat gaaaagtgct ttttgctaaa atccgccggt agccatcaat caaccgccat
      121 gccaatttcc cagcccgttg gccaacccgc cctgacccgc agtgaacgca aagatatcac
      181 ccgtgccagc ctggtgcagg ccgctctggc cttgattggc aaggggcgca gttttaccag
      241 tctgggaatt cgcgagatcg cgcgcgaagc ggacctggtg ccgaatgctt tttaccgcca
      301 ttttcgcaat gtcgatgagt tggggctggc attggtggag cagcttggca tcaccttgcg
      361 ccgcctgttg cgtgaggccc gtcagaccgg ggtggctccc aacgccatgg tgcaccgctc
      421 ggttcaggtt tatctggagt acgtcaggca aaaccgcttg cagtttctgt tcatctccag
      481 cgagcgctcg ggcggtagcc gcagcctgcg cctggccatt cgccaagacg tgaatcaatt
      541 caccaatgaa atggcgcagg acttccgccg tctcggcatg ttccccgaca tgcccactag
      601 cacgctgcaa atggtctgcg ggctgatcgt gaccaccatg cttgcagcag cgcccgagat
      661 tttggatttg ccacccggtc agcctttgct ggaggcgcaa atgaccgaga actttgtcct
      721 gcaactgcaa ctggtgttgc tgggtgcgtc ttgctggaaa gacccggcgg cgcgcaaaat
      781 ttcaagccaa attagcctct agcccttgcg ggtattgcgc tgacagcttc gataaagata
      841 gtaaaaagtg ccacttcatt gcatttccag atcattcgtg tcttggcacc ctgccacagg
      901 caatgcaaac gcatggcagg gcaaggcagg gcgaggcatg gcaagataac aaggcaagat
      961 agcaaggaaa tggatgattc aaaaaatgca atcagatgcc aaccagggtc atctggccgc
     1021 gtgttcttct ggcttgttga tcacccggat atcggttaat tcaatccaga ccagcatcgg
     1081 cgcgggcagc acaagctggt cgccaaagcg cggtgtcacg cgacgccggg tgtaaaagcg
     1141 caagccgtcg acccgctccc agttcaggca gcggttggcc gctgtggccc aggagccgat
     1201 gaccgtggcg gtgtagtcat ggcgcagcag caggccggca tcatcgatat ggaaagtttc
     1261 cagtaccgaa tgggatggca cggcaggttt aaattcggca cgcaacatgc gcgagccgct
     1321 ttcaccgcca gcatcgatgc cggtcagggt cacgccttca ttcgcgaggt aaaacggaaa
     1381 acacaagtag ttccaaagcg ccttgccagc aaagtacaag atatcgagtt tgtcccaggc
     1441 cagccgcttt ttcagggtga agaaggcggc gtaagggttg tcgcgctgcg ctaccagctt
     1501 gccgtcgcca tcttgcagga gaacttgttg cggtgtccag atgccgcacc attccggctt
     1561 ggcaaaaccg cccaaagtta cccgctgctg gtgcgggtaa accgaaatcc tcaggttctt
     1621 cagggccgag ccttgctgtt tcatggaaaa cgccaagcca tatgacgaca gcgctgcctc
     1681 aatcactgcc acttgttgcc agcgttcggc cccgccatgg gcggtgatgg cagttgtcag
     1741 catgtcagcg gcggctgggc tgctgacttg gcggccttgg gtaaattggc ttggattggt
     1801 cacggcgttc tcgctcgggt aaaaagtgcc agggctgtgg cggagtttgt gtctgactcg
     1861 atttccaatg ccattgccaa atcatccctg tcgttgttgt ctggtcttgc cttgcgcttg
     1921 caaagtcgat gccagggtgc ggacacgaat gattgagaaa tggaaagaga aatggacaaa
     1981 ttgtcagcaa aggcactggc aaggtcaatt cagacccgcc ttgtggcttg tgtctgccag
     2041 cgcgggctac ccaggcagtc gcaaaacact acaaaataag tagctgcctg cgcaatctgg
     2101 tcaagggcta gagccctatt ttgcctaata gtccttggtg tcggcccaca tgctgggctt
     2161 gaagcgaatg acacggttgc gcccctgttc cttggcctgg tagagcgcaa tgtcggcaaa
     2221 cttgacggct tgccagaaag tttcactgtt ggctggaaaa tctgaaatgc caatcgaaat
     2281 ggtcttgcac aactctatgg tgccgacttt gaccttgaga ccttcaactg ccagacggat
     2341 gctttcggcc acccggtcgg ccgcctctcc agcactgtca accaaaatga tcaggaattc
     2401 ctcgccacca tagcgaatca ccaggtctga ggcgcggacc gactgtttca gcaccgtgga
     2461 cagggcttta aggactgcgt caccggcatc atggccgtag gtgtcgttga ccatcttgaa
     2521 gtaatccagg tcaagcatca agatcgccac atgggcgctc ttgcgctgca cattggcaac
     2581 cagggtatca acgtactcct ccagaaaacg gcggttgtgc aggccggtca tcgggtcctg
     2641 cagggttgag tcgcgcaggc tttccatcag gcgtttggtt tccagcaccg gggcggcttc
     2701 acgcagatag acattgatga acggcgtctt gtcctgcaag gtttgctgat cggctggccc
     2761 agccagcaac tgcaccacgc tgcccacagc acccgactgg atcaccggaa aacaaaaatg
     2821 gcggcgatcc ccaagttcgg ccggcggcat gaagctgttg cagatgtcag cgtgaacaat
     2881 gccattgacc aggtgaccgg tgcggcgcac ccggcaggct tccgggcgca ccagaatctg
     2941 cgggttacac cagcggcaac tatcggcagc ttcgccatcg accaaaatgg gcttcatctg
     3001 ttttttgttg ccgctgacct catagatcga gaactcagtc agcctgaatt cgtttttcag
     3061 ggtgttggcc aggcgtcggt agatttcggt cttggattca tcctcttcaa tggcttgctt
     3121 gaaatgcgct gctttggtca gtccttcgac catgtcaatg gtcgccgtca gcaggttttc
     3181 acctggggcc ggggtgcgtt cggtcaggcg tgccacatgg ctgccgatgc ggttgaggcc
     3241 attgtcaaga aagcccagca ggcgattcat gtcggtggca atctgtccga tctcatcgct
     3301 ggtgcgcttc tccacctggg cctggaagtc acccagcaac gcacgctgaa ccgcatcttc
     3361 gaccgcattg gcggtgtccg agatggggcg cagcagccga cgcagcagca aaatcatcag
     3421 caaagaaaaa acgatcaccg ccccggcaat gctggcgatg gtgaagaggg ccttttcttt
     3481 cagtgcatca atcgacatgc tcatgctgac agcacctagc accgtgccct ccggtacttg
     3541 atggcattgc aggcagttgg gcgtgccggt cgcggtggca atatagggga tggtgccgcg
     3601 aaagacggtt tcgtcgcttc gggacaaaag ttcaaagcgt gccttgcctt cgcgcatcac
     3661 cgccagttca atctcatcgg caatcgactc gaccgccagg ccctggccga actgctttgc
     3721 cacttcaggc gagcgaatca cgcgcgccga tttcagaccc tgaatctcga ccaaacgcaa
     3781 caagaaacgt tcacgcttgt cgatcacgcc attgatcatg gattcggtca ggtgcacccg
     3841 cacaatctcg ccggcggtac ggatgtgctc tgtggcagag gtgatggaaa agctgcgtaa
     3901 cgcgtacacg ctgatggcga tcagcaccac cagcagaccg gcggcaatgc tgacgaaaaa
     3961 gagggtgact ttggcgttga gattcacgtg aaaaacttca aggagccctg ccatacgtca
     4021 gggccgcaag attgtctcac acgcaatcgg gcagccattt tgccggtctt gacccggctc
     4081 agagaaggcg cggtgaaggc acccgacacc ctgttcgcct ggccttgcgt tgcaagctgc
     4141 tggcacaacc ggccgcagtg ccggatgtcg caaccctact gtgcggcccg gcgcagggtc
     4201 tgcaccaccg gacggcgcag cacgtcgcgc aggccccacc aacccgccgc cagcgccagc
     4261 acggcaccgg ccagcgcacc cagcaagggc acccacaatg acaccaccca ggtgaactca
     4321 aacacatagc gcgccaatgc ccagcccacc acgctggcca ccagcgaggc cagcaagccc
     4381 gccagcaagc caacaccgat caactcgatg cgctgcacct ggcgcagcaa actggcgcgg
     4441 gccccgaccg cgcgcatgat ggcgaactca cgcgcgcgct cttcgcgggt ggcggtgact
     4501 gccgcaaaca gcaccaccag cccggccgcc agggtgaagg caaacagaaa ttccaccgcg
     4561 ccaaccactt gatcgagcac ccgctgaatc tgggcaatgg tggcagtcat gtcaacgctg
     4621 gtgatattgg gaaactggcg caccagcgcg ttatcgaacc cggctgtgac cggtgccttg
     4681 aaagcgctca tgtaggtggc gggcaaatcg tcgagcttgc cgactgggta catggcatag
     4741 aagttggccc gcatggaacc ccagtccacc ttgcgcagcg aggtgatcct ggagtcggtt
     4801 ggcacgccac cgacatcaaa acgcagtcgg tcgccaatcg acaggccgag ggttttggca
     4861 atgccttcct cgatgctgat ggcacccgcc tcgtcgtcct gccaacgccc ggccaccacc
     4921 agattttgct tgggcagagc ggcactgtgc gacaggttga actcgcggtc aatcagccgg
     4981 cgggcgcgct cgtcgatgaa gtcgtccggg ctgacatcgc ggtcgttgat ggccaccagt
     5041 cggccacgaa tcattggaaa ccagtcaaac tgcagcacgc cagcgtcttt gagcgtttgg
     5101 gaaaaggcac cagcctgatc ggccatgatg ttgatgacaa agcggtttgg cgcatcggcg
     5161 ggcgatgcct ggcgccaact gttgatcaaa tcggtgcgca gcagcaccag cagcaccagc
     5221 gccagcagac cgaccgccag actgctgacc tgcaccacgg caaaagccgg gcgggccgag
     5281 atttgccggg ttgccagcac caatgcggtc ggggcggtgg tgtcgttgac gaaacgccgc
     5341 agcagcttga ccgccagcca gcttaaggcg gcaaacagca gcgttgcgcc agcaaagccg
     5401 ccaaccgcaa tcaggcccaa cttgacatcg ctgctggcag ccatcagcag cgcggcaaat
     5461 ccggccacgc ccagacccaa caccagcagc gagattggct tgaggctgcc gacatcgcgc
     5521 cggatcaccc gtagcggcgg cacttgtgcc aattgcagca ctggcggcag cccgaaagcc
     5581 agcagcaagg tcagtcccat gccaaggcca aatagcaccg gccagatgcc gggtgtcggc
     5641 aaaccggcat ccaccagacc agccagcaag gcgataaagc catagtgaac ggcaaacccc
     5701 agcgccacgc caaccgcgct ggccagcagg ccgatcagca caaactcaaa gctgtaggcc
     5761 gcagcgatgg tgcgctgcgg caaacccagc acccgcagca tggcacagtc gtcgaggtgg
     5821 ttggcagcaa aaccgcgcgc cgccagcgcc actgccaccg cgctcagcag cgccgccagc
     5881 agggcgacca gattcaggaa tttctccgca cgctccatcg tggtgctgag ttccgggcga
     5941 ccgccctgca aggcttcgac ccgcaaaccg cgcgcggcgc gggcctgaac ctgcgcctgc
     6001 gaccaatcga caaacgcttg caccgactgc tcttcaccgg ccaccgccat gcggtaactc
     6061 aagcggctgg cgggttgaac caggccagtg gcagccaggt cggccaggtt gaccatcacc
     6121 cgcggtgcaa agttcatgaa acccgcgccc cggtccggct cggtgacgat caactgggca
     6181 acttgcaggc tgctgtcgcc cagcaacagg gtgtctccca ggcgcaggtt caaggcctcc
     6241 agcaatgccg gttcaatcca gacctggccg ggcgctggca ccgcacgcgt gagtgcaccg
     6301 gcggagccag gtgtggaact gatgcgcaaa ttgccgcgca aggggtaacc ggcctgcacc
     6361 gccttcaggg ccaccaagtt gctctgtccg ccttgcgcat ccggcgcgcg taccatggtc
     6421 gggaaggtca gggtctggac ctgctgcaag cccagcgttc gggcttgcgc caggaacgca
     6481 gccggggtcg ggttgtcact gaccaccacg gcatcgccgc ccagcagttg gcgtgcatca
     6541 cgctgcaagc cactcttgag gcggtccgcg aaaaaaccca ccgcagtcag tgcggctacc
     6601 gccagcgtga ccgccaccat cagcaaacgc agctcaccgg cgcgcaaatc acgcgtcaaa
     6661 gagcgccagc ccagcttgaa aaatgaaatt gtcatgctgg cacgataccg cagtcaggcc
     6721 gcatctgcca ccggcagtcg ctttcaaccc gcaatgatca gggtaaaggt cggtcccgtc
     6781 agagcgccat cggcggcaac caatggaccc gggcggcggt agcgctcgac ccaggcagat
     6841 cgccatgcag cgacccgtgg tgcgatttca ttttcagcac caaatcaggc tgtagcccaa
     6901 tcaatgcgag cgctgaaagc tataaaaata agagcaaatc agcggcccac ataaggcctt
     6961 tgccgtccat ttcggggttg tgctggcggg atttgccgtc ttgcgtgctg gcgcacccgc
     7021 ctttgccatg agtctgcctt tttacagacg acaaaaagat tgaggaatag ttcactaaaa
     7081 gtgcccccag cccttatgga taaagcgctg ccagctatca attcaatatt tcaacggttt
     7141 gtcaccgttg atccagatca aaatatcagt caacactgat ctatacaatg cctgcactgg
     7201 cgcgcagcat cggcttgacc cgccggacgc acaccggggt ttttgcaagc aactcaagga
     7261 aatcaccatg ttgaacccct cgcaacaggc actcaaagcc gtcagcctgg cgctgctgct
     7321 gtgcctcggc ccgctggcgc aagccgcaga cgcggtgacg caagccatgc aaactgccaa
     7381 tggcccgtac cgcatggcgc tgttcaagac caacggcact tcgcaagccg agtcgcagca
     7441 ggcgctgacg caggcgatgc aggcctggga caagctcagc agcgaatttg ctgccaaacc
     7501 cgccgcacct tacgaccgtg acccggcctt tgccagctcg gttgcggcgg tgtcaaaggt
     7561 ttacgccaaa gcgcagggcg agatcagtgc caacgacctc aaggcggccc acagcacgct
     7621 ggaagaagcg cgcgagatca tgtctgccat gcgcaaacgc aacaacgtgg tggtttacag
     7681 cgaccacatg aacgcctacc acgaggtgat ggaacacatt ctggtggacg gcaatgccct
     7741 gctggcccag cccaagggtc tgttggtgat gacggcacaa gccggtgccc tgagctatgt
     7801 ggccaaacgc ctgagcacac aagcacccga gagcctggca aaagacgcag agttccaggc
     7861 cctggtggat gccgtcaagc aatcggttga ccggctggag tcggcattgc aaaaccagga
     7921 ccaagccgct gtcaaggacg caatgggaaa gctcaaaggc ccgtacagca agctgtttgc
     7981 caaattcggt tgattttttg tttcaaggag tgtttgatga gtacgtttga cgccattggc
     8041 ctgattaaag gtatcaatga aaacctgggg ccgtttcgca agtcgcagtc agaagccatg
     8101 ctgggctttg gtcagttggc caaggcatcc atggcagagg gcgcggtcag cgccaaaaac
     8161 aaggaactga tcgcactggc cattggcatt acccagcatt gccacggctg cattgccttt
     8221 cacgtcaagg cgctgcgccg actcggttgc agccgggccg agcttgaaga aacacttgcc
     8281 gtgtgcgttt acatgggcgg tggccctggc ttgatgaccg ccgccgatgc actcagcgcc
     8341 tgggatgaga tggagtctgc ctgacagccg tgccgccggg gtcggctgcc gtaaatcagc
     8401 ggtgccgatt cggtcagcgc ggtgcggcaa ccgggcctgg cgtaacgcga gccgatagcg
     8461 gggcgcgcca cgcctggact ttactcaatg gtcagcgacg cacctcatcg accagcgcct
     8521 tgaactcgtc aatgtcttca aaactgcggt agacgctggc aaagcgcacg taggcaacct
     8581 tgtcgagttt cttcagttcg agcatcacca gttcaccgat gcgggccgac aacacctcac
     8641 gaaggcccag gctcagcagt tgttcttcga tgcgctcggc cgcactgtcc accagttcag
     8701 tgctgaccgg gcgcttgcgc aacgccagtt tcatggaagc cagcagcttg ttgtggtcgt
     8761 actcaatgcg acgcccgtcc ttcttgacaa tcgccggaaa agtcacctcg ggccgctcat
     8821 aggtggtgaa gcgcttttcg cacgacatac aacggcgtct gcggcgaata aaacccccgt
     8881 cttccgacac ccgggtctcc gcgacctggg tctcaggatg gctgcaaaag gggcatttca
     8941 tgctgccgcc gctggcgtga tgatgccagg cgtggcaatc caggactggt ggatgatgcg
     9001 aatagccagc ttgccgggtg cctcgccagg acggctgatc acgccgagta aacaggaaag
     9061 cgtgcagtca gcgcgttgac catggcgcga acggcatcaa tgttgctgct gtcccttggg
     9121 ttgtcaagca cgtcagcgac cagatgcgca gtgatgcggg cttcttcgtc cttgaagcca
     9181 cgggtcgtca tggccggggt gccgatgcga acgccgctgg tcaccatggg cttttcgggg
     9241 tcgttgggaa tggcattctt gttgatcgtc atatgggcgc tgcccagcac cgcttcagct
     9301 tccttgccgg tgatgccctt ggcgcgcagg tcgaccagca tcacatgtga ttcggtccgg
     9361 ccgctgacga tgcgcagccc gcgttcggtc aaggtttcgg ccacgatgcg ggcgttcacc
     9421 agcacctgct gctggtacag cttgaattcg ggctgcaatg cttccttgaa ggcgactgcc
     9481 ttggcggcaa tcacatgcat cagcggacca ccttgcaggc cggggaagat ggcgctgttg
     9541 atgacttttt cgtgctgcgc tttcatcaag atgatgccgc cacgcgggcc gcgcaagctc
     9601 ttgtgggtgg tgctggtgac cacgtcggcg tgtggcaccg ggttggggta gatgccagcg
     9661 gcaatcagcc cggcatagtg cgccatatca accatgaaga tggcaccgac atctttggca
     9721 actttggcaa aacgtgcaaa gtcaatagca agagaataag cactggcacc cgctatgatc
     9781 agttttggct tgtgttcatg ggctttgcgc tccatcgcgt cgtagtcaat tgcttcgctg
     9841 gcatcgaggc cataagacac cacgttgaac catttgccgc tcatgttcag cgccatgccg
     9901 tgggtcaggt ggccgccttc ggccaggctc atgcccatga tggtgtcgcc gggctttaga
     9961 aaagccagaa acaccgcttc gttggctgat gcaccgcaat gcgcttgcac attggcggcc
    10021 tcggccccaa agatctgctt gacccggtca attgccagtt gctcggcaat gtcgacaaac
    10081 tcgcagccgc cgtagtagcg cttgccaggg tagccctcgg cgtatttgtt ggtgagctgc
    10141 gtgccctgcg cggccatcac cgccggtgaa gcatagtttt cgctggcgat cagctcaatg
    10201 tggtgctctt ggcgggcgtt ttcagcctga atggcggaga acaattcggg gtcggtttgc
    10261 tcgatcagaa tattgcggtt gaacatggca gtccttaaag acaatgggcc tgtgagtcat
    10321 ggtgcatgaa aacagggctg cccaggcgaa cggcaaaacg catcacccaa acaggttttg
    10381 cggcacgctc ccccgtggtg ttcccacttc agcgcccaaa gacccgattg ggttgggcac
    10441 ctatcgccag ttgcgtgcga ggcgaatttt agccgaccga tttattgcag ggccaccgtg
    10501 gccactttgt ctggctcggc ttcaggtgcc gcatctgcag cagcaggcgg caaggcgcgg
    10561 atgaccgggg cgctcggatt tgatgtggtg ggtggcttgc ggccaagtgc aacttgctgc
    10621 agtttggcgt gttcggccat gactttcgca gcgtagccgc catcgagctc gacattggca
    10681 gcaccgacat agtatttcag gccaccttca accgagcccg cgacccggat gcagtcctgc
    10741 aacaccttga caccgacatg caaattggca aaggggtcaa aggcggcaaa cttgccgcca
    10801 aagctctggt acttgtcact gtgaacgcgg gtcatcacct gcatcaggcc ctgggcaccg
    10861 accgggctct gggcaaacgg gttaaagcct gattcgatcg ccatgactgc caggatcagg
    10921 gttggttcga gccgggtctg actgccaatt tcataggcct cggacaccag cgcgctgatc
    10981 ggttcgggcg cgacgttgta ttttttgctc agccagaaag cgactgcggc ctgttgcttg
    11041 gggagatctg ccgggtcggt ggcagtggca cgttcgcttg ccaccgggtc actgaccagg
    11101 ccggtgacga tcacctggcg ctcttgcagc cagccaaaca gtttgacttc gcccaactca
    11161 cgcaattcgg ggcgagccag caaggccatg gtggcaaata gtacagccag gccgatcagg
    11221 gcgaagctgt tgtgggtgac ttcaaaaaag cctttggtga cgacggtagt caagcgtcgc
    11281 aggcggatcg tcatggtatg ggtggctgac atagcttttc cttcttgctc ggggttaagg
    11341 gggctgcgtg ggttacacac agcgcgacca aggcctcgcc tgggttgtta cgctatcaat
    11401 atcctgctgt atcctttctt tgatgatgca gagacttgat gttgccgaca gtcggcagcg
    11461 ggttgaaaga tcctgagtaa ctcaagattg gcggattcta gggggacttg ttatgccaag
    11521 tcaaccatat caaataaact ccttatgtca aaaaaaattg taactgtcgt cgatcactcg
    11581 gcaaaatgcc gcttcattca cgcaaaattt atgccatgaa cttcgccata ttctcccgtt
    11641 tgtcaccttc ccggcagcaa tggctgcacc gcgccggggc ctttgtgctt ggtctgctgc
    11701 tactttggct gctggcctgg ctggcggtgc cgcccattct caaatcgcaa gtccagaggc
    11761 ttctgggcga gcaattaggc cgggcggtca gcattgggca gatcgacttc aagccctgga
    11821 cgctggaact cgatgttcag gacctggcgg ttgcgcatgc cgcaaccgcc accgaccgac
    11881 cggcttctgc aggtgagccg gtcgtcaagc cccaagtgca catcaagcgc ctgtatttcg
    11941 atctggaact gcagtcgctg ttgcgcctgg ccccggtggt cgatgcactg acgatagagt
    12001 cgcctgcgct ccagttgcgc catcttggac agggtcgcta cgacgtggac gatgtgctga
    12061 ccaggttggc cagccgaccg accaacccgg atgcagcacc gctgcgcttt gccttgtaca
    12121 acctggtgtt gcaggatggc gggctggatt tcaccgacga cgcgcagggt aaggtgcacc
    12181 gcctgagcgc tttgaaaatc agcctgccgt tcctcagcaa tctcgacgcg ctgcgagacg
    12241 ttcaggtgca acccttgttg gcgtttgacc tgaacggcag ccggtttgac tcgaccgcgc
    12301 ataccacgcc atttgcgcag acccgcaaga ctgaggcaca gttttccctc aagcggctgg
    12361 acctggcacc ctatctgggc tattggccag ccagcctgcc agcgcgcctg acttcggccg
    12421 tgatcgatgc tgacctgaaa ctggtgtttg agcaagcgca gcagccccat gttgccatca
    12481 gcggcctgct gtcagccagc ggcgtgaagt tgcaggcaac aggcaaagga acggcggcat
    12541 tggccgtgcc cgaactgctg gcgtttgaca agctggcggt gcagttcaag tcgctgcgcc
    12601 cgcttgagcg ggtggcccaa ttgagccgtg ttgactggca gcagccgctg ctgacgctgc
    12661 accgaaacaa gcagggtcag gtgaactggc tggccttggc aaaggcccag cagacacctt
    12721 ctaatgcttc taaaacagta gcttccagcg cagacagggc aagggctaca ggccaaaaac
    12781 aagcagaaac cagggcagac gcaaggcctg ccggatggca gcttggcatt gatcagattg
    12841 cccttgaagg cggtgaaatc cgttggcttg acgatgctgc tgctgcgccg gtgcgcctgg
    12901 cattgaccgg actgaccttg caggcacagg cgctggcctg gccgtttacc cagccgatgc
    12961 cgtttcaggg cgctggcagg ctcaattcgg ccacgctcac tttcaagggc gatgcaaccg
    13021 accaggcggc ggatgtcagc gtcagcctgg ccgacctgcc gctgtcgctg gcggcaccct
    13081 atctggccag ccagttcatc cccacgctcg acgggatgct aacgaccgaa ctggcactga
    13141 actggaaggc cgccagcgcg actggcgcgg gggcgcaaat cctgctgaaa gtgcccagcc
    13201 tgaccctgga caagctgctg ttgtccgacc cccgcaaggc gcgcctggcc agcatcaaac
    13261 aagtgcggct ggagacggct gaactcgatg tggcgcgtca aaccgtcacg ctggcccggc
    13321 tgcggctgga ccagccgcaa ggccatctgg cacgcgatgc acagggccgc tggaactttg
    13381 aagattggcg caaggaccag ctcgcagtgc aggctgcggc accgtcagcc cagcctgcca
    13441 cgggcactac cagaaccact accaaaacca ctgccgccgc agcagcgcca ctgcctgcag
    13501 ccgtcgctcc tgtttccgct ggcgcgccag cagatgcggc ccggccgtgg ctggtcacgg
    13561 tgcaagactt tgcgctgtca gacgcaagcc tgggctttca ggatatggcc catggaaagg
    13621 ccgtggcgct ggacctgtca gccctgtcgc tgcagcttaa aaacttttcc agcgctgctg
    13681 gcaagccttt tggcctgacg ctggcagcac gggtgcgcca cggcaacacc gagcctggcc
    13741 aactgtcctg gcgcggcact ggcagcttga gccccctgag ccttcagggc gatgtgctgg
    13801 cacagcggct gccagcacac gcgctggctg cctattttgg tgatgccttg aatgtggcac
    13861 cgctgcggct tgatgccagt ttcaagggca agcttgattt ggcccagcaa agcgctggtt
    13921 tggcgctgca ggtgcgtggc gatgcccgcc tggaagacgt gctggtgcac agccttgccg
    13981 ccacccaggg tgcaacggca accccggcag ccaatgcgcc cgcgcccg
//
LOCUS       DAOSLL010000039        13856 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4507_length_13856_cov_5.162162, whole genome shotgun sequence.
ACCESSION   DAOSLL010000039 DAOSLL010000000
VERSION     DAOSLL010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 13856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13856
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4507_length_13856_cov_5.162162"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..569
                     /locus_tag="PLB25_02870"
     CDS             87..569
                     /locus_tag="PLB25_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE40567.1"
                     /translation="MRQRGFTLLELLVVLAIVAMASAGVGFAMRDSGQARLEREAQRL
                     VALLESARARSQALGIPLRWRVTVSGFRFEGASQETAEQALPDQWLDADTRASIEPSG
                     NSVAATTRPSPANADTLLLGPEPIIEPQTVLLSSVSRPQQHVRIATDGLRPFAPQDAP
                     "
     gene            569..964
                     /gene="gspI"
                     /locus_tag="PLB25_02875"
     CDS             569..964
                     /gene="gspI"
                     /locus_tag="PLB25_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HOE40568.1"
                     /translation="MAGLVQTAGVGQTARRARGFTLVEVLVALGIVAIALMAGLQASA
                     ALTRHAGRQSDMLLAQICAENELVRLRLARQLPGVGDTQVPCQQAERTLTVELVVRPT
                     PNPNFRRVDARVLDAELPIWRLTTVVGRY"
     gene            964..1638
                     /locus_tag="PLB25_02880"
     CDS             964..1638
                     /locus_tag="PLB25_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE40569.1"
                     /translation="MQADRPRRSGGFTLVELLVAISLMALMALLSWRGLDGIGRTQSR
                     LLQRSDEVLTLQATLAQWSADLDAMTPQPNLPGLEWDGRSLRILRRGSIDAADGLRVV
                     AWALRVVNGQGHWLRWLSPPLQNRADLDLAWQKAAIWAQTPSDEDHLREVRTVPLDSW
                     QIFYYRSNAWTNPLSSADSDTAAGTGTAATLPDGVRLLLKLPPGQALGGSLTRDWVQP
                     LLGGSR"
     gene            1635..2663
                     /gene="gspK"
                     /locus_tag="PLB25_02885"
     CDS             1635..2663
                     /gene="gspK"
                     /locus_tag="PLB25_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HOE40570.1"
                     /translation="MRRRPLVNRAGQRGAAILTAMLTVVLVATLASAMLWQQWRGVEV
                     ESAQRSRVQAAWILTGALDWARLILREDARQGGADHLAEPWAVPLAPARLSTFLSAER
                     GQALVTEGDDAESEAFLAGHIVDLQARLNVTNLIDNGKLHQPTVAVWARLFKHLNLPE
                     QELLALTQSLLRAQASGAAAPASSAASATPATAAVTAATALLPQAVPELVWLGLSATT
                     LQTLQPFITVLPERTVVNLNTAPPEVLLASVKGLDMAQAQALVQARAARHMDTIMDAD
                     RLVGNPQVQFDSAEQGVTSRFFSVTGQLKLGQTTLQEQSVLQRDGLQVKALSRVREQV
                     GADARPLQ"
     gene            2763..3020
                     /locus_tag="PLB25_02890"
     CDS             2763..3020
                     /locus_tag="PLB25_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40571.1"
                     /translation="MHPLELLTTRLAALRLLARSSPHRRRTSQLAASCKAQPCHKAGL
                     PKPDQLESNSLRRGGATATEHLKYFEFVGVTPARRMTLWLS"
     gene            3182..4471
                     /gene="gspL"
                     /locus_tag="PLB25_02895"
     CDS             3182..4471
                     /gene="gspL"
                     /locus_tag="PLB25_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HOE40572.1"
                     /translation="MSLLIITLPIDCASPTALFDYVQSPDGSTVSAHASVPLALLPTT
                     PTRLGEVVLLLPARALSWHQVQLPQGSLPRQVFGERGTTRLRAILDGLLEDQLLDEPA
                     QYHLALQPQADTSAPVWVVACARSWLEDGLRTLAQADHVVTRIVPEFSVQALADTIYV
                     TEQAQAAQLVGLLRQGSASHASTGVLVTTLSATTVGLLEGAPAPGDASAVVGEPACRV
                     VAEPAVAAQAEALFHRPVELQQVAQRLLQAAQTRWDLAQFALARTGRNRRWRSLSAGL
                     RGFAGATAWRAARWSLLALFAVNLLGLNALAWREQANLQAQRQHIRSVLTDTFPKIPV
                     VVDAPLQMAREVALLQRSRGNPASTDLESILASFSALAPDGYALTAIEYAANELRLTG
                     PQMDPKAVSRLLDGLKSAGLNGRLQGDQWLIGPGAAR"
     gene            4441..4947
                     /gene="gspM"
                     /locus_tag="PLB25_02900"
     CDS             4441..4947
                     /gene="gspM"
                     /locus_tag="PLB25_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HOE40573.1"
                     /translation="MADWAGGSAMNWRGTWQARWQAASTREQGLLLLALALVGLALLW
                     WLALAPALAVLKRADLQHQQLGTQLQQMQQMQAQAQALQALPTLDAREARSALEAALK
                     PLGSAAQISTQMDRLTVTLKGVEASALVQVLSVARQNAHLAASEARLKRAASGAWDGS
                     LVFTLPAQ"
     gene            4947..5795
                     /gene="gspN"
                     /locus_tag="PLB25_02905"
     CDS             4947..5795
                     /gene="gspN"
                     /locus_tag="PLB25_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HOE40574.1"
                     /translation="MASRPNARFQSAAAVLRVVRRPRAGAATPWGWAAAGVLLGLLAS
                     LLLFAPARWLAGGLQQASAGRLLLQDARGTLWTGSARLSLGGGAGSSAAAALPGRLAW
                     QLRPTLASPGLALQLQADCCLAQAWRWHLSPRWNGLQMVFSDHQSHWPAQSLAGLGTP
                     WNTLQLQGQLLLETQALGLSWSSGRLQVAGRAQLDVQDLSSRLSTLRPMGSYRLTLSG
                     GATPELLLATESGSLQLSGRGQWVGGRLRFVGEASSTPESQAALSNLLNIIGRRNGAR
                     SIINLS"
     gene            5802..7916
                     /gene="gspD"
                     /locus_tag="PLB25_02910"
     CDS             5802..7916
                     /gene="gspD"
                     /locus_tag="PLB25_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HOE40575.1"
                     /translation="MTHSPPTGFQHAILSIAVSALLTGAGGLFSINTWAQAGAAGKPG
                     EPVTLNFQAAEIEAVARTFASITGRNVVVDPRVKGVITLVTEKPVSPAVAMNQFVAAL
                     RLQGFTLVESAGLYKVVPEADAKLQTAGVTVSGGESAGLPSGNQIVTQIYRLNHESAN
                     NLVPVLRPLISPNNTINANPGNNSLVITDYADNLRRLGRIIAAMDVANATDVEVIVLR
                     HAIASDLAPLVLRLIGSNAAAAPAGQADTWYKPILVAEPRSNSLILRAANAAQLAMVR
                     SLVAKLDQPDSQGGNGAAGNIYVVYLKNADATKLATTLRAALSGEARAGAAAPVATPG
                     GAPTAAATALTHSTGGQIQADAATNSLIITAPEPQYRQLRAVIDKLDARRAQVFVESL
                     IAEVSTDKSADFGIQWQGALGSSGDSVIGLLGTNFGVGGKNIIDLATGAESGTVLPDR
                     GLNFGAAIKTNGIYVLGFLARFLEDSGSGNVLSTPNLLTLDNEEAKIVIGQNVPFVTG
                     QFTNTTSTTGTVNPFQTIERKDVGLTLKIKPQISENGTVRLTIFQEVSSVLASTVNAV
                     NGPTTNKRTIESSVLVDDGSIVVLGGLLQDEYSGNAEKIPGLADVPLLGNLFKSESRS
                     RKKTNLMVFLRPVVVRDARDSDKLSLDRYELMRSGLQDNQSPASAMMPAIGSPQLPAA
                     TRKAGASPTALPLAEPLVPAKP"
     gene            7943..9343
                     /gene="gspE"
                     /locus_tag="PLB25_02915"
     CDS             7943..9343
                     /gene="gspE"
                     /locus_tag="PLB25_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HOE40576.1"
                     /translation="MRYPLPYAFARSHQLLLEEDGEQLTLWLHTQSAPAAVAEVLRKY
                     PHSQLQAEGPAALVQRISAAYAQGESSAAAVVNEVESDADLSRMMQDLPAIEDLLETA
                     DDAPIIRMLNALLTQAARDGASDIHIEPYERHSSVRFRVDGTLREVVQPNRALHAALI
                     SRLKIMAELDIAERRLPQDGRISLRIGTRAVDVRVSTLPSAHGERAVLRLLDKSEGRL
                     SLEALGMQGEVLRRFEKLVAQPHGIVLVTGPTGSGKTTTLYAALSRLDASRNNIMTVE
                     DPIEYELPGVGQTQVNPKIELDFAKALRAILRQDPDIIMIGEIRDFETAQIAIQASLT
                     GHLVLATLHTNDAASAVTRLTDMGVEPFLLSSSLLGVLAQRLLRKLCVQCQHKQRPVG
                     CPHCGQTGYSGRSGVFELLVTDDAIRAQIHNRAAEADIRHAAQASGMVLMREDGERLV
                     QLGLTSREELLRVTRD"
     gene            9366..9902
                     /locus_tag="PLB25_02920"
     CDS             9366..9902
                     /locus_tag="PLB25_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40577.1"
                     /translation="MNTCQPSCKGGIVVVARRFGVKPSGSCPHRQLAGRAVVVARRMG
                     VRAQFGDAVLARLSAFGRIGIRPQFCYRVPIIGAAEKAWAGYQFCYRVPVIGAAEKAG
                     AGYQFCYRVPAIGAVDKAGAGYQFCYRVPAIGAAENAGAGYQFCYRVPVIGAAEKAGA
                     GYSTLYFQRHQRHAGLFL"
     gene            9883..11112
                     /gene="gspF"
                     /locus_tag="PLB25_02925"
     CDS             9883..11112
                     /gene="gspF"
                     /locus_tag="PLB25_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HOE40578.1"
                     /translation="MPVYSFEALSSAGDTRKGVIEADSAKAARGQLRAQALVPLKVEQ
                     VSAQEDASANGSRASARRGKRVFGATALAIWTRQLAGLVSSGLPLERALTSLSDEAEQ
                     APERELVATLRAEVNGGAPFAKALSQHPREFSPIFVAVIGAGEQSGNLALVLERLADD
                     LEEQQALKAKLIGAALYPAIVSLVAVVIVLFLVTYVVPQVAHVFAGSKRALPLLTVLL
                     IGISSFVRSYGWLLAIAIFIGAAGARLALANAQFHEKFDAAWLNLPIVGRLSRSYNAA
                     RFASTLAMLAAAGVPILKALQAAAETLSNRALRSDAQEALVLVREGAPLASALAQKKR
                     FPPLLAMFARLGEQTGQLPVMLQRAAKQLGNEVQRRAMQLATLLEPLLIVAMGVVVML
                     IVLAVLLPIIQLNQLVK"
     gene            complement(11441..13597)
                     /locus_tag="PLB25_02930"
     CDS             complement(11441..13597)
                     /locus_tag="PLB25_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOE40579.1"
                     /translation="MPQNPTTGLNLAQQEAVNYLHGPCLVLAGAGSGKTRVITHKIGR
                     LIQSGMLARRIFAITFTNKAAAEMRERARGLIGRAAKDAVICTFHALGVRLLREDGAA
                     LGLKTNFSILDSDDVTSILKDAGGTVDAATARQWQWTISRWKNQGLNAERAQAAAASD
                     SDHLIARVMGHYEERLAAFQSVDFDDLISLPLKLLQNHELIRQKWQALAGHILVDEYQ
                     DTNATQYELLKLLVGGAGPAQARFTAVGDDDQSIYGWRGATLDNLKRLPVDFPGLKVI
                     KLEQNYRSTSAILRAANNVIGPNPKLFPKNLWSDLGEGEPVRVVDADTEEHEAERTVA
                     RIQSLRINGLPQPGPQFTELRDFAVLYRANHQAKTFEKALRKAQIPYKVSGGTSFFDR
                     AEIKDLCGWFRLWINSHDDPAFLRAVTSPKRGIGHQTLAHLGDFAGKYKLSMFDALFA
                     SSLGAVISGKALGSLHEFGREVTDLEYRARQTLGAPAAKAFLLQWLSDIGFEKHLYDG
                     EDSEKVAAARWTNVLDFCDWMAARCGGQIADAAGVSSAGEVKSLLEVAQTVSLLSTIN
                     EQEKDQNVVTLSTLHAAKGLEWPHVMLVGVTEGMLPFKLRDQPDDAAAGQTSDAQRAA
                     AQEAANATSTVHLQEERRLMYVGITRAKASLAVSWTRKRKKGREMVVAQPSRFIAEMA
                     LDQSTVKEDPRAKLKALRAEFAQKALDGEAARALLR"
     gene            13649..>13856
                     /locus_tag="PLB25_02935"
     CDS             13649..>13856
                     /locus_tag="PLB25_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOE40580.1"
                     /translation="MLHIFTITFPFFALVLCGYLAARRHLLPLEAIAGLNRFVLFFAL
                     PCMLYRFGAGTPIAQLLDVSVALTW"
BASE COUNT         2362 a         4453 c         4488 g         2553 t
ORIGIN      
        1 cagttccatt gggctctgac cccaattctg cgggccatgc ccagattgtt tcctgctcca
       61 aaatttattg caaaacctcg tcaggcatgc gccagcgcgg ctttaccctg ctcgaattgc
      121 tggtggtgct ggcgatcgtg gcgatggcct cggccggtgt cggctttgcc atgcgcgaca
      181 gcgggcaagc ccggctggag cgcgaagccc agcgcctggt ggccttgctg gagtcggccc
      241 gggcacgctc gcaggccctt ggcataccgc tgcgctggcg cgtcaccgtg tcaggctttc
      301 gctttgaagg cgcaagccag gagactgccg agcaggcttt gccagaccaa tggctcgatg
      361 ccgatacgcg cgccagcatc gagccctccg gcaacagcgt cgccgccacc accaggccgt
      421 cgccagccaa tgccgatacc ttgctgctcg gcccagagcc gatcattgag ccccagaccg
      481 tgctgctcag tagcgtcagc cgaccgcagc agcacgtgcg catcgccacc gacggcttgc
      541 gaccgtttgc gccgcaggat gccccgtaat ggctggtctg gtgcaaaccg caggagtcgg
      601 ccagactgcg cggcgcgcgc gcggcttcac gctggttgaa gtgctggtgg cgctgggcat
      661 tgtggcaatc gccctgatgg ccggtttgca ggccagtgcc gcgctaacgc gccatgccgg
      721 gcgccagtct gacatgttgc tggcgcaaat ctgcgctgaa aatgaactgg tgcgcttgcg
      781 gctggcccgg caactgcccg gtgtcggtga cacccaggtg ccgtgccagc aggccgagcg
      841 cacgctgacg gtggagctgg tggtgcgccc cacgccgaat ccgaacttcc gccgggttga
      901 tgccagagtg ctcgatgccg agctgccgat ctggcgcttg accaccgtgg tggggcgcta
      961 ctgatgcagg cagacaggcc acgccggtca ggcggcttca ccctggtgga actgctggtc
     1021 gccatcagcc tgatggcctt gatggcactt ctgagctggc gcgggcttga cggcattggc
     1081 cgcacccaaa gccgcttgct gcagcgctct gacgaggtgc tgacgctgca ggccacgctg
     1141 gcccaatgga gcgccgatct ggatgcgatg accccgcaac cgaacctgcc cggcctcgaa
     1201 tgggacggcc gcagcttgcg catcttgcgc cgtggcagca tcgatgcggc tgacggtttg
     1261 cgggtggtgg cctgggcgct gcgggtggtc aacgggcagg gtcactggtt gcgctggctg
     1321 tcgccgccgc tgcaaaaccg cgccgacctg gatttggcct ggcaaaaagc cgctatctgg
     1381 gcgcaaaccc ccagcgatga agaccacctg cgcgaagtgc gcaccgtgcc actggacagt
     1441 tggcagatct tttattaccg cagcaatgcc tggaccaacc cgctgtccag cgccgacagc
     1501 gacaccgctg ccggtaccgg caccgcagca acgctgcccg atggcgtgcg gctgctgctg
     1561 aaattgccgc cggggcaggc gcttggcggc agcctgacgc gcgactgggt gcagccgctg
     1621 ctgggaggca gcaggtgagg cgccggccgc tcgtcaaccg ggctggccag cgcggtgccg
     1681 ccattttgac cgccatgctg acggtggtgc tggtggccac gctggcatca gccatgctgt
     1741 ggcagcagtg gcgcggtgtc gaggtcgagt cagcccagcg cagccgggtt caggccgcct
     1801 ggattttgac cggcgcactg gactgggcgc ggctgatcct gcgcgaagac gcacgccagg
     1861 gcggtgccga ccatctggcc gaaccctggg cggtaccgct ggccccggca cggctgtcca
     1921 cctttttgtc ggccgaacgc ggccaggcgc tggtgaccga aggtgacgac gctgagtcag
     1981 aggcttttct ggccggtcac atcgttgacc tgcaggcccg cctgaatgtc accaacctga
     2041 ttgacaacgg caaactgcac cagcccacgg tcgcagtctg ggcacgcctg ttcaaacacc
     2101 tgaatctgcc cgagcaggaa ctgctggcgc tgacccagtc cctgctgcgc gcccaggcca
     2161 gtggcgctgc tgcgccagct tcgtcggccg cgtcggcgac accggcgaca gcggcggtga
     2221 ccgcggcgac agcgctgctg ccgcaggcgg tgccagagct ggtctggctg ggcctgtcag
     2281 ccaccacact gcaaaccttg cagcccttca tcaccgtgct gcccgagcgc accgtggtca
     2341 acctcaacac cgcgccgccc gaggtgttgc tggccagtgt caaagggctt gacatggcgc
     2401 aggcgcaagc gctggtgcag gcacgcgcgg cccgccacat ggacaccatc atggatgccg
     2461 accggctggt gggcaacccg caggttcaat tcgactctgc cgagcagggc gtgacctcgc
     2521 gctttttcag cgtcaccggg cagctcaagc tgggccagac cacgctgcaa gaacagtcgg
     2581 tgctgcagcg cgacgggctg caggtgaaag ccctcagccg ggtgcgcgag caagttggcg
     2641 ctgatgcccg gcccctacaa tgaccccaga ttcctcagtt gaccgcttgt tttcatcggt
     2701 aagtccttat gagtatgtgc tttttcagct ttggtggggt tcacccaagg ggtcccggcg
     2761 cgatgcaccc gcttgaactg ctgacaacgc gcctggcggc attgcgcctg ctggcgcgca
     2821 gcagcccgca tcgtcgtcgc acctcgcaac ttgctgcttc atgcaaggcg cagccatgcc
     2881 acaaggctgg cctgcccaag cccgaccaac tggaatcgaa cagccttcgt cgcgggggcg
     2941 cgactgccac ggaacatctg aagtattttg agtttgtggg agtcacaccc gcgcgacgaa
     3001 tgacgctatg gctgtcctga attcttcccg gcaattgcaa agacttgact tgtcagtgag
     3061 tcaagacgtg ccaacctgcg caggcagctt tgaagctgcg gctgtcgcgc tgcacagcca
     3121 caccacaagc ggccgagtcg gacacctgca gcctggtaat cccggctgat tcgcggcttc
     3181 catgtccttg ctgatcatca ccttaccgat tgactgcgcc agcccgacgg cgctgtttga
     3241 ctatgtgcaa agccctgatg gcagcaccgt gtcggcgcac gccagcgtgc cgctggcctt
     3301 gctgccgacc acgccgacgc ggctcggcga ggtggtgctg ttgctgcccg cccgggcctt
     3361 gtcctggcac caggtgcaat tgccccaggg cagcttgccg cgccaggtgt ttggcgaacg
     3421 tggcacgacg cgcttgcggg ccattctgga cggcctgctg gaagaccagt tgctcgacga
     3481 gccggcccag tatcacctgg ccctgcagcc gcaagccgac accagcgcgc cggtctgggt
     3541 ggtcgcctgt gcgcgcagtt ggcttgaaga tggcctccgg acgctggccc aggccgatca
     3601 tgtggtgaca cgcatcgtgc cggaattctc cgtgcaggcg ttggccgaca cgatttacgt
     3661 caccgagcag gcgcaggccg cgcaactggt gggcttgctc aggcagggtt cggccagcca
     3721 tgccagcacc ggcgtgctgg tgacaacgct gtcagccacc acggtgggct tgctggaggg
     3781 cgcgcccgcg cctggcgatg ccagcgcggt ggtcggggag ccagcttgcc gggtggttgc
     3841 cgaacctgcc gtggcagcgc aggccgaagc cctgtttcac cgaccggtcg aactgcagca
     3901 agtggcccag cgcctgctgc aagcggccca gacgcgctgg gacctggccc agtttgcgct
     3961 ggcccgcacc ggccgcaacc ggcgctggcg ctctctgtcc gcgggcttgc gcggctttgc
     4021 cggtgccacc gcctggcgcg ccgcgcgctg gtcgctgctg gcgctgtttg cggtcaacct
     4081 gctcgggctg aacgccttgg cctggcgtga acaggccaac ctgcaggcgc agcgccagca
     4141 cattcgcagc gtgctgaccg acacctttcc caaaatcccg gtggtggttg atgcgccact
     4201 gcaaatggca cgtgaagtgg cgctgctgca gcgcagccgt ggcaacccgg cgagcactga
     4261 tctggaaagt attttggcat cattttcggc tctagcgcct gatggctatg cgctgacagc
     4321 tattgaatat gcagcaaatg agctacgcct gacggggccg cagatggacc ccaaggcggt
     4381 ttcaaggttg cttgacgggc tcaagtcagc gggcctgaat ggccgccttc agggtgatca
     4441 atggctgatt gggccggggg cagcgcgatg aactggcgcg gcacctggca ggcgcgctgg
     4501 caagcggcca gcacacgcga gcaaggtctg ctgctgctgg cactggcgct ggttggcctg
     4561 gcgctgctgt ggtggctggc gctggcaccg gctttggcgg tgctcaagcg tgccgacctg
     4621 cagcaccagc aactcggtac gcaattgcag caaatgcagc agatgcaggc gcaggcgcag
     4681 gcgctgcagg cactgccgac cctcgacgcg cgggaagccc gcagtgcgct tgaggcagcg
     4741 ctcaagccgc tcggcagcgc cgcccagatc agcactcaaa tggaccgcct gacggtcacg
     4801 ctcaagggtg ttgaggcaag cgcgctggtg caggtgttgt cggtagcccg gcaaaacgcc
     4861 catctggccg ccagcgaggc gcgcctgaaa cgcgccgcct ccggggcctg ggacggcagc
     4921 ctggtgttca ccttgccagc ccagtgatgg ccagccgacc gaacgcccgt ttccagagcg
     4981 ctgcggcggt gctcagggtg gtgcgtcggc cgcgtgcagg cgccgccacc ccctggggct
     5041 gggcggcggc cggtgtgctg ctgggtttgc tggccagtct gctgctgttt gcgccagcgc
     5101 gctggctcgc tggcggcctg caacaagcca gcgccgggcg gctgctgctg caggacgcgc
     5161 gcggcactct gtggactggc tcggcccgcc tgagcctggg cggcggtgcc ggcagcagcg
     5221 ctgccgcggc cctgcccggc cgactggcct ggcagttgcg cccaacgctg gccagccccg
     5281 gcctggcctt gcaactgcaa gccgattgct gcctggcgca agcctggcgc tggcacctgt
     5341 cgccgcgctg gaacggcttg cagatggttt tttccgatca tcagtcgcac tggccggcgc
     5401 aatcgctggc aggcctgggc acaccctgga acacgctgca attgcagggg caactgttgc
     5461 tcgaaaccca ggcgctgggc ttgagctgga gcagcgggcg gctgcaggtg gccgggcgcg
     5521 cgcaacttga cgtgcaagac ctgtcgtcgc ggctatcaac attgcgaccc atgggcagct
     5581 accgcctgac gctcagcggc ggcgcgacac ccgagctgtt gctggccacc gagagcggca
     5641 gtttgcagct ttcgggccgt ggtcagtggg ttggcggcag gctgcgcttt gttggcgaag
     5701 ccagcagcac acccgagtcg caagcggcct tgtcgaatct tttgaacatc atcgggcgac
     5761 gcaacggtgc gcgctcgatc atcaacctga gttgaaactg catgacacac tccccaccca
     5821 ccggttttca acatgctatt ttatcgatag ctgtcagcgc tttattgacg ggggctggcg
     5881 gccttttttc catcaatacc tgggcgcagg ccggggctgc gggcaagccg ggcgaaccgg
     5941 tcacgctgaa ttttcaggcg gccgaaatcg aagccgtggc gcgcaccttt gccagcatca
     6001 ccgggcgcaa cgtggtggtg gacccgcgtg tcaagggggt catcacgctg gtcactgaga
     6061 agccggtcag cccggctgtc gccatgaacc agtttgtcgc ggcgctgcga ctgcagggct
     6121 tcaccctggt cgagtcggcc gggctttaca aggtggtgcc cgaggccgac gccaaactgc
     6181 agacagccgg ggtcacggtc agcggcggcg aatcggctgg cctgccttcg ggcaaccaga
     6241 tcgtcacgca gatttaccgg ctcaaccatg agtcggccaa caacctggtg ccggtgctgc
     6301 gcccgctgat cagccccaac aacaccatca atgccaaccc cggcaacaac tcgctggtga
     6361 tcaccgacta tgccgacaac ctgcgtcgtt tggggcgcat cattgccgcg atggatgtcg
     6421 ccaacgccac cgatgtcgaa gtgattgtgc tcaggcatgc gattgcgtct gacctggcac
     6481 cgctggtgct gcgcctgatc ggcagcaacg ccgcagcggc accggccggg caggctgaca
     6541 cctggtacaa gcccatattg gtggccgagc cgcgctccaa ttcactgatt ttgcgggccg
     6601 ccaatgccgc ccagttggcg atggtgcgtt cgctggtggc caaactcgac cagcccgaca
     6661 gccagggcgg caatggcgcg gccggcaata tctatgtggt ctatctgaaa aacgccgatg
     6721 ccaccaaact ggccaccacc ttgcgtgcgg cactcagtgg cgaggcccgc gctggcgcag
     6781 ccgccccggt ggccacgccg ggcggggcac caactgccgc cgccaccgcc ctgacccaca
     6841 gcaccggcgg ccagatccag gccgatgccg ccaccaattc gctcatcatc accgcgcccg
     6901 agccgcaata ccggcaacta cgcgccgtca tcgacaaact tgatgcccgg cgcgcccagg
     6961 tgtttgtcga aagcctgatt gccgaggtca gcacggacaa gtcggccgat ttcggcatcc
     7021 agtggcaggg tgcgctgggc agctccggtg acagcgtgat tggcctgctg ggcaccaact
     7081 ttggcgttgg cggcaaaaac atcattgacc tggccaccgg ggccgaaagt ggcacggtgc
     7141 tgccagatcg cgggctcaac tttggcgcgg cgatcaaaac caacggcatc tacgtgctgg
     7201 gttttctggc gcgctttctg gaagattcgg gcagcggcaa cgtgctctcg acccccaatt
     7261 tgctgacgct cgacaacgaa gaagccaaga tcgtgattgg ccagaacgtg ccttttgtca
     7321 ccggccagtt caccaatacc acatcgacca ccggcaccgt caacccgttt cagacgattg
     7381 agcgcaagga cgtggggctg acgctcaaga tcaaaccgca aatcagcgag aacggcaccg
     7441 tcaggctgac cattttccag gaggtttcga gcgtgctggc ctcaaccgtg aatgccgtca
     7501 atggcccgac caccaacaag cgcaccattg agtcaagtgt gctggtcgat gatggcagca
     7561 ttgtggtgct cggtggcctg ttgcaggatg agtactcggg caacgccgag aaaatcccgg
     7621 ggctggccga cgtgccgctg ttgggcaatt tgttcaagag cgagtcgcgc tcgcgcaaaa
     7681 aaaccaacct gatggtgttt ttgcgcccgg tggtggtgcg tgatgccagg gactcggaca
     7741 agttgtcgct ggaccgctat gagctgatgc gctccgggct gcaagacaac cagtcgccag
     7801 ccagcgccat gatgcctgcc atcgggtcgc cgcagttgcc cgctgccacg cgcaaggccg
     7861 gtgccagccc gaccgcgtta ccgctggccg aaccgttggt gcccgccaaa ccgtaacgcc
     7921 aattgacctg ccgccaaaag ccatgcgcta ccccctgccc tatgcctttg cccgcagcca
     7981 ccagttgctg ctggaagaag atggcgaaca actgaccttg tggctgcaca cccagtctgc
     8041 gccagccgca gtggccgaag tgctgcgcaa atacccgcac agccagttgc aggcagaagg
     8101 cccagcggcg ctggtccagc gcatcagcgc ggcttacgcg cagggcgaat ccagtgccgc
     8161 agcagtcgtc aatgaggtcg aaagcgatgc cgacctgtcg cgcatgatgc aggatttgcc
     8221 agccattgaa gacctgctgg aaacggctga cgatgcgccc atcatccgca tgctcaatgc
     8281 gttgctgacg caggccgcac gcgacggtgc cagcgacatc cacattgagc cctacgagcg
     8341 ccattcgtcg gtgcgttttc gtgttgatgg cacgctgcgc gaggtggtcc agcccaaccg
     8401 cgcgctgcac gcagcgctga tttcgcggct caaaatcatg gccgaactcg acattgccga
     8461 gcgccgcctg ccgcaggatg gccgcatctc cctgcgcatt ggcaccaggg cggttgatgt
     8521 gcgggtcagc accttgccca gcgcccatgg cgagcgcgcc gtgctgcgcc tgctcgacaa
     8581 atccgaaggc cgcctgagtc tggaggcgct gggcatgcag ggcgaggtgc tgcgccgttt
     8641 tgaaaaactg gtggcacaac cgcacggcat tgtgctggtg accggcccga ccggttccgg
     8701 caagaccacc acgctgtacg cggcgctgag ccgactcgat gccagccgca acaacatcat
     8761 gacggtggaa gacccgatcg agtacgagct gccaggggtc ggccagaccc aggtcaaccc
     8821 gaagatcgaa ctcgactttg ccaaggcgct gcgcgccatc ctgcggcaag acccggacat
     8881 catcatgatc ggcgaaatcc gcgacttcga gaccgcgcag atcgccattc aggcatcact
     8941 caccggccac ctggtgctgg ccacgctgca caccaatgat gcggccagcg ccgtgacgcg
     9001 cctgaccgac atgggggttg agccgttttt gctgagttcg tcactgttgg gtgtgctggc
     9061 gcagcgcctg ctgcgcaagc tgtgcgtgca gtgccagcac aagcagcgcc cggttggctg
     9121 cccgcactgc gggcagaccg gctacagcgg gcgcagcggt gtgtttgagc tgctggtgac
     9181 cgacgacgcg attcgcgcgc agatccacaa ccgggcggcc gaggccgaca tccgccatgc
     9241 ggcacaagcc agtggcatgg tgctgatgcg tgaagacggc gagcgcctgg tgcagttggg
     9301 gctgacctcg cgcgaagaac tgctgcgcgt gacccgtgat taagcactgg ccattgcatc
     9361 gcatgatgaa cacttgccag ccaagctgca agggtggcat cgtggtggtg gcccgcagat
     9421 ttggggtcaa gccgtcgggt agttgcccac atcgccaact ggcagggcgc gccgtggtgg
     9481 tggcccgcag aatgggggtc agagcccaat tcggggatgc cgtgctggca cgtttgagtg
     9541 cgtttggccg aattgggatc cgaccccaat tctgctaccg cgtgcccatc attggcgctg
     9601 ccgagaaagc ttgggctggc taccaattct gttaccgcgt acccgtcatc ggcgctgccg
     9661 agaaggccgg ggctggttac caattctgct accgcgtgcc tgccattggc gctgtcgata
     9721 aggccggggc tggctaccaa ttctgctacc gcgtgcctgc cattggcgct gccgagaacg
     9781 ccggggctgg ttaccaattc tgctaccgcg tacccgtcat tggcgctgct gaaaaggccg
     9841 gggctggata ttcaactttg tactttcaac gccatcagcg ccatgccggt ctattccttt
     9901 gaagccctca gcagcgctgg cgacacccgc aaaggggtga tcgaggctga ctcggccaag
     9961 gcggcgcggg gccagttgcg tgcgcaggcg ctggtgccgc tcaaggtcga gcaagtcagc
    10021 gcgcaagagg acgcgtctgc caacggcagc cgggcctcgg cacggcgcgg caagcgggtt
    10081 tttggtgcga cggcgctggc gatctggacg cgccagttgg ccgggctggt gtcatcgggc
    10141 ttgccgctgg agcgcgccct gacttccctg tcagacgaag ccgaacaggc ccccgagcgc
    10201 gaactggtgg cgacgctgcg cgccgaggtc aatggcggtg cgccgtttgc caaagccttg
    10261 tcgcagcatc cgcgcgagtt ttcgccgatc tttgttgcgg tgattggtgc tggcgagcaa
    10321 agcggcaacc tcgcactggt gctggagcgc ctggctgacg acctggaaga gcagcaggca
    10381 ctcaaggcca aactgattgg cgcggcgctc tacccggcca ttgtgtcgct ggtggcggtg
    10441 gtcattgtgc tgtttctggt gacttatgtg gtgccgcaag tggcccatgt atttgccggc
    10501 agcaagcgcg ccctgccgct gctcaccgtg ctgttgattg gcatcagcag ctttgtgcgc
    10561 agttacggct ggctgctcgc aattgctatt tttataggag ctgccggcgc aagactggcg
    10621 ctggctaacg cccaatttca tgagaaattt gacgcagcct ggctgaatct gccgattgtg
    10681 ggtcgcctgt cgcgcagcta taacgcagcg cgctttgcca gcaccctggc gatgttggcc
    10741 gcagccggcg taccgattct gaaagccctg caggcggcgg ccgaaaccct gtcgaaccgg
    10801 gccctgcgca gcgacgcgca agaagccctg gtgctggtgc gcgaaggtgc gccgctggcc
    10861 tctgcgctgg cgcaaaagaa gcgttttccg ccactgctgg cgatgtttgc ccgcctgggc
    10921 gagcaaaccg gccagttgcc ggtgatgctg cagcgcgccg ccaaacagct cggcaacgag
    10981 gtccagcgcc gcgccatgca gttggccact ctgctcgaac cgctgctgat tgtggccatg
    11041 ggcgtggtgg tgatgctgat cgtgctggca gtgctgctgc cgatcatcca gctcaatcaa
    11101 ctggtgaaat agccgggcat ttcctgccca caagcagcag caacccccca ggcgcgttgc
    11161 cagcggccct cgggtgcgtc caactaaggt tcaatatgtg taaaccgcct gacagcgcac
    11221 cagacgcgcc agcaaagcct gagattggca gcgcgggaaa ccagcagcgc agcctgacgg
    11281 ttgtcgatat ttttggctgt ttttgcgctt tagcccccgt gtctaatgcg ctggaagcta
    11341 tcgactttat agctatccgg agcccagggc ggactttacc ggtgaccacc attggcctgg
    11401 caatgtcagc aggcgcagct cagggctgga gtgcccccga tcatcgcagc agcgcccggg
    11461 ctgcctcgcc atcgagcgcc ttttgcgcaa attcggcacg caacgccttg agcttggcgc
    11521 gcgggtcttc tttaacggtg ctttgatcca gtgccatctc ggcgataaag cggctgggct
    11581 gggcgaccac catctcgcgg ccttttttgc gtttgcgcgt ccagctcacc gccagactgg
    11641 ctttggcgcg ggtgatgcca acgtacatca ggcggcgctc ttcctgcaga tgcacggtcg
    11701 atgtcgcgtt ggcggcctcc tgcgctgctg cgcgctgtgc gtctgatgtc tgaccagcgg
    11761 cagcatcgtc gggctggtcg cgcagcttga acggcagcat gccttcggtc acacccacca
    11821 gcatcacatg cggccattcc agccctttgg cggcatgcag ggttgacagc gtcaccacgt
    11881 tctggtcttt ttcctgctcg ttgatggtgg acaatagcga taccgtttgc gccacctcca
    11941 gcagcgattt gacctcgcca gcgctgctga caccggccgc atcggcaatc tggccgccgc
    12001 agcgcgccgc catccagtcg caaaaatcca gcacgttggt ccagcgcgca gcggccacct
    12061 tttcgctgtc ctcgccatcg tacagatgct tctcgaaacc aatgtcactg agccattgca
    12121 gcaaaaaagc cttggccgcc ggtgccccga gcgtctggcg ggcgcgatat tcaaggtcag
    12181 tgacctcgcg accaaactcg tgcaggctgc ccagcgcctt gcccgaaatc accgccccga
    12241 ggctgctggc aaacaacgcg tcgaacatgc tgagcttgta tttgccagca aagtcaccca
    12301 ggtgcgccag ggtctggtgg ccaatgccgc gcttggggct ggtgacggcg cgcaaaaagg
    12361 ccgggtcgtc gtggctgttg atccacagcc gaaaccagcc acacaagtcc ttgatttcgg
    12421 cccggtcaaa aaagctggtg ccacccgaca ccttgtacgg aatttgcgcc ttgcgcagcg
    12481 ctttctcgaa agtcttggcc tggtggttgg cccggtacag cacggcaaag tcgcgcagct
    12541 ccgtgaactg cggaccgggc tgcggcaagc cgttgatgcg caggctctga atccgcgcca
    12601 cggtgcgctc ggcttcgtgt tcttcggtgt cggcatcgac cacccggacc ggctcgcctt
    12661 cgccaaggtc actccacagg ttttttggaa acagcttggg gttcgggcca atgacattgt
    12721 tggcagcccg caggatggcg gaagtggacc ggtagttttg ctccagcttg atgactttga
    12781 ggccaggaaa atcgaccggc agccttttca ggttgtccag cgtggcaccg cgccagccgt
    12841 aaattgactg gtcatcgtcg ccgacagcgg taaagcgcgc ttgcgccggg ccagccccac
    12901 cgaccagcag cttcaacagc tcgtactggg tggcgttggt gtcctggtat tcatcgacca
    12961 gaatatgccc ggccagagct tgccacttct gcctgattag ctcgtgattt tgtagcagct
    13021 tgaggggcag gctgatcagg tcgtcgaaat cgacgctctg gaaggcggca agccgttctt
    13081 cgtaatgccc catcacacgg gcaatcaggt gatcgctgtc actggcggca gcggcctggg
    13141 cgcgctcggc gttcagaccc tggtttttcc accggctgat ggtccactgc cactggcgcg
    13201 cggtggcggc atcgaccgtg ccaccggcat ctttcaggat gctggtgaca tcgtcgctgt
    13261 cgagaatcga aaaattggtt ttcaggccca gcgccgcacc gtcctcgcgc agcagacgca
    13321 ccccgagcgc gtgaaaggtg cagatcaccg cgtctttggc ggcccggcca atcaggccac
    13381 gcgcccgctc acgcatttct gcggcagcct tgttggtgaa ggtgatggca aaaatgcgcc
    13441 tggccagcat gccggactgg atcagtcggc caatcttgtg ggtgatgacc cgggttttgc
    13501 ccgagcccgc accggccagc accaggcacg gaccatgcag gtaattgacc gcttcttgct
    13561 gggccagatt gaggccggtg gtggggtttt gaggcatgcg gtgcaaacgt gtgagggggc
    13621 cgaatcatac tcaagtgaca atctccgggt gctgcacatc ttcaccatca cctttccgtt
    13681 ctttgccctg gtgttgtgcg gctacctggc ggcgcgccga cacttgctgc cgctcgaagc
    13741 gattgccggg ctgaaccggt ttgtgctgtt ttttgccctg ccgtgcatgt tgtaccgttt
    13801 tggtgctggc acgccgattg cgcagttgct tgatgtctcg gttgcgttaa cctggc
//
LOCUS       DAOSLL010000040        13855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4508_length_13855_cov_5.153188, whole genome shotgun sequence.
ACCESSION   DAOSLL010000040 DAOSLL010000000
VERSION     DAOSLL010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 13855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13855
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4508_length_13855_cov_5.153188"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            102..593
                     /locus_tag="PLB25_02940"
     CDS             102..593
                     /locus_tag="PLB25_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOE40581.1"
                     /translation="MTPVELDTVDLQLLKHLQIDSSQSNQALAELVHVSPPTCLRRVK
                     RLRDAGLIEREIAVLSVDKIASVIGHGLCAVVEISLDRQDQAALLAFEARVLAEDAVQ
                     QCYRVSPGPDFCLVVHAADMPDYLALTQRLFTQDANVRNVKTFFSIKRSKFGARLPLP
                     MSV"
     gene            complement(1269..2105)
                     /locus_tag="PLB25_02945"
     CDS             complement(1269..2105)
                     /locus_tag="PLB25_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE40582.1"
                     /translation="MSSAQGPSGPDVTAAAVPTAAHDVTAAAVPAGGRDATLESLQRQ
                     LDQARAELQEFTYAVSHDLRAPLRHISAFALIIEEDLPDLPVDIAGHLATIRQSAHLL
                     TQQLDGLASLSRLGLQAVHLQPTSSALLVAEVIDELVQKYPQRALQWQIAPDLPMLMA
                     DASLLRQVIANLLENACKFTKARQPAQISVSWAPLAGGLCALTVQDNGVGFLPRQAGQ
                     LFKVFARLHPVREFEGLGLGLVQCRKMMDRMGGSIRIAAVADGGCQVSLTLPMADGGV
                     PS"
     gene            complement(2102..3535)
                     /gene="glmU"
                     /locus_tag="PLB25_02950"
     CDS             complement(2102..3535)
                     /gene="glmU"
                     /locus_tag="PLB25_02950"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HOE40583.1"
                     /translation="MTSNLDIFPAMPVDVVIMAAGKGTRMKSKLPKVLHLLAGRALLQ
                     HVVDLAGALAARQVTVITGHGATEVEAAIAQSTGATSQFHLNFVRQEPQLGTGHALQQ
                     AVPVMQDDGVVVVLSGDVPLTQPDTLAQLIAACAGQRLALLTIDLANPGGYGRIVRQG
                     EVVQAIVEQKDATPAQRLISEVYSGIMAVPARLLKPWLARLDNKNAQGEYYLTDVVKF
                     AVADGVAVVAHKTSDATQVAGINSPVQLAQLERACQRRIAAQLMEQGVRLIDPARLDV
                     RGTLKCGQDVVIDVNCVFEGQVTLDEGVQVGANCVIKNASIAAGAVIHPFSHIEGGTP
                     GSKDAVEVGAGALIGPFARLRPGAKLGGGVHIGNFVEVKNSTLAKGAKANHLAYLGDA
                     TVGERVNYGAGSITANYDGANKHRTIIEADVHIGSNCVLIAPVTIGAGGTVGGGTTLT
                     KDTPPLALSVARARQVSIANWKRPSKK"
     gene            3561..4463
                     /locus_tag="PLB25_02955"
     CDS             3561..4463
                     /locus_tag="PLB25_02955"
                     /inference="COORDINATES: protein motif:HMM:NF039714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6279 family lipoprotein"
                     /protein_id="HOE40584.1"
                     /translation="MSWRIISALLTLLGALLLGGCSVARLGYSQAPDLVYWWVDSYLD
                     LSDAQSSRLRADLVALHQWHRSTELPLLAGSLADLQGVALQDTTPVRLCQLVDALRPR
                     LQAVLDQAEPGFATLAVSLKPEQLAHLQRQLEKRQQKWRKDWLDGSPTERLERRTERL
                     IDRSEMFYGRLDASQRALLRARVAASGFDSTLAEADMLRRQQDLLQTLQAIGRSGLGT
                     PRAQDDIHQLLARLLLPAQPAERARQELLRQENCGTFAALHNSTSPAQRRQLVETLKA
                     FESDARALMPGTSPPKMVSINHRN"
     gene            4465..4875
                     /locus_tag="PLB25_02960"
     CDS             4465..4875
                     /locus_tag="PLB25_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40585.1"
                     /translation="MSLRFIAAVTRLIQADEQLLGGRVTSVQHAALREAALLKALKEL
                     AMQSGVELELSMSKDGKGDITLVAKPEPGQSAKPGPGHFGQAFVDRLNAFLPVTGRRP
                     NAMLLPERGKCQMNHLDAEKMVMACFDELKRQQP"
     gene            complement(4876..5244)
                     /locus_tag="PLB25_02965"
     CDS             complement(4876..5244)
                     /locus_tag="PLB25_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40586.1"
                     /translation="MEIHKLAKKSHWSIRLGQPEWSKFTYQRPADDPLKLLGSARHGH
                     QYGALAKLEDELVLVVGDYITALGKSDQKQLADLLANAKIPDELPVYQPPRPREGPPP
                     VVIIKRRRIPVLPVPAVSRD"
     gene            complement(5310..6578)
                     /locus_tag="PLB25_02970"
     CDS             complement(5310..6578)
                     /locus_tag="PLB25_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid deaminase"
                     /protein_id="HOE40587.1"
                     /translation="MLDPLIDSSFKGFPHHSAPLHRSHIAAQGWNVLLGDLPLPLAVI
                     KRDELQHNLRWMQDFATSHGLGLAPHGKTSMSPQLFRHQLDAGAWGMTFATLTQARIG
                     VAAGATRILIANQVFAEVDLAGIDALKRLHPELRLLFLVDSVAQLELIEAWRAHRPAA
                     LAFEVLLEIGVVGGRTGCRTSEQALTLAEWLHASSAVQLVGLACYEGLAATGQSAQDE
                     AYVHKLMELLQAVARACDARNLFDADEVLLTAGGSAIFDLVVGGLRLSLSRPVTGLLR
                     SGCYVTHDQGTYKRMMSALADRTGCGTGLQGAMEVWASVQSCPEPGLAILGLGKRDIS
                     FDWEMPTPLLLCRHGSCQPQAAAPDWRISKLNDQHAYLTGTGPAHAALQVGDRVCLGI
                     SHPCTTFDKWRWIPIVDQRYTVVDAIITCF"
     gene            complement(6650..7249)
                     /locus_tag="PLB25_02975"
     CDS             complement(6650..7249)
                     /locus_tag="PLB25_02975"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HOE40588.1"
                     /translation="MNNSDEKRASDKRALRKTLVDRRLSLPDRQQRVDLLQRVMRIWL
                     VGRPDTVIGAYWPIKGEFDPLPALHRWKEDGELVDRPRLRKIGLPVVDKVHKTMSFHA
                     WYPGCPMEDDAFGIPKPKDTELITPTLLFVACVGYAPGGYRLGYGGGFYDRTLAGLSP
                     RPFTVGLGFATDFLPDFEPEPHDMALDAILNDHGVVWPV"
     gene            7306..9240
                     /locus_tag="PLB25_02980"
     CDS             7306..9240
                     /locus_tag="PLB25_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOE40589.1"
                     /translation="MVALPATAPAASRADDVLLEMNSAFQKGQGKRLTELLPQAKGHI
                     LEPWAAYWELRARLDNASADEVEGFLSRYRGSYQEDRLRNDWLLLLGQRRDWGRFASI
                     APDFRMNDDREVRCYTLAMEQILAGVDLSEDVKTQWYRQKEGGAACALAAEKHHAAKK
                     LTDADIWRKARLAVEAHQFKSARQAVLIVAPDADGALSELAASPERFLSRLTPSAKPQ
                     AQELVVLALIRLADSAPDRAAVELEKRWGRYLTGPQRAWVWGSIGKQAAQKLSDDALG
                     YFSHASPADMSDEHLAWRVRAALRQNQWHDALKAMQAMSADTADDAAWVYWRARAMLQ
                     PGRTDAEQVQARQLLQSIAGLGGFYEILAQEELGLPIAPPATPEPLSTAEKDNARANP
                     GLQRALAAVALGLRAEGVREWNYSTNLHAPGGMNDRELLAAADLACQHEVWDRCINTS
                     DRTRSVIDLNQRFPMPHKKAVLARTRDIGLDPAYVYGLIRQESRFIMDARSGVGASGL
                     MQVMPATAKWTAKKIGMNDFKVQQITDRDTNIAIGTGYLKLVLDDFAGSMPMAAAAYN
                     AGPGRPRSWRGNSGAPAIEAAIWAENIPFNETRDYVKKVLANTTIYAAMISGKAQSLK
                     ARLGLVGPREIKVPENRDLP"
     gene            9350..10033
                     /locus_tag="PLB25_02985"
     CDS             9350..10033
                     /locus_tag="PLB25_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HOE40590.1"
                     /translation="MLKLYIGNKNYSSWSMRPWVLLKQAEIEFEEVMVRFDAFTPDSQ
                     FKTTISKINPVAKVPVLVDGVLVVWDTLAIAEYLAEQFPQKQLWPADRAARARARSLC
                     AEMHSGFSALRSACPMNIEASLAQVGALIWRDQPAVRADVARLVAMWQALLGEHGGPL
                     LFGDFSVADAYFAPICTRLITYALPLPPDLASYAARVCALPGVKAWIDQARAEHDFLD
                     FEEPYRLKP"
     gene            10035..11348
                     /locus_tag="PLB25_02990"
     CDS             10035..11348
                     /locus_tag="PLB25_02990"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HOE40591.1"
                     /translation="MQIYLVGGAVRDALMGLPVKDRDWVVVGSTPDELISHGFVPVGK
                     DFPVFLHPRTHEEFALARTERKTAPGYHGFAVHAAPDVTLEEDLARRDITINSIAVSA
                     YSIRDSGQFDVKVSGEPETWLGTGALVDPHGGVQDLARKVFRHVSPAFREDPVRILRV
                     ARLAARFADFTLAPETLTLMRAMAEDGEVDHLVAERVWQELARGLMENTPSRMFEVLR
                     DCGALVRLLPEVDRLWGVPQRADYHPEVDTGVHLLLVLNMCARLQASLPVRVACLFHD
                     LGKGNTPPDVLPRHIGHEERSARLLKVVCERLRLPTECRELADVVAREHSNIHRSDDL
                     GASALVRLLERCDALRKPQRFAELLLACECDVRGRHQHSEQAYPQRQRLLRVLAVMQQ
                     IDTKSIAAKAITAGATGLKVGELIHKARIAAVQANCQDLQPVLSS"
     gene            11650..12357
                     /locus_tag="PLB25_02995"
     CDS             11650..12357
                     /locus_tag="PLB25_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE40592.1"
                     /translation="MSSLLIIEDDELLRDGLCAQLAQAGHSVTAAADGEAAQALLEKR
                     RFDGVVLDLGLPKISGMDLLHWVRKRLPALPVLILTARCGVDDRVQGLNAGADDYLTK
                     PFEMAELQARLGALLRRARLPAFGGTLEVASNTTRRLRVDASLPQAWLGDEALELTQR
                     EWALLSLLVTQAGRVVSREDVLETWSAEPGEGGGAGVASNALEVYIHRLRRKLVDSGL
                     NIRNVRGLGYMLESTAA"
     gene            12399..13802
                     /locus_tag="PLB25_03000"
     CDS             12399..13802
                     /locus_tag="PLB25_03000"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HOE40593.1"
                     /translation="MASVAAPRQGLSLKRQLLLWLLAPQLVLFLVGGALAYRIALSYA
                     EKAIDQSLTQSVRSLARQVKPVGSGLLIDFPRAAQDIIEQDPKDRVSYMVSSPPGHFL
                     LGNGKLPDPIGSAPLLTNEAVLYNTRVDGKAMRVALLEVDYGDAQSPQRMRVQVAKGL
                     AVQQRLTTELIADMLAPLLLLGALLSMLVYGGISRGLQPLTRLQAQLGDRQLADLSAL
                     SPIEMTQAPHEVHALAAAVNQLLSAVRRSLGQEKRFLSDAAHQLRTPLAGLISQTELA
                     LTESDPAALRERLTKVLAGAQRSAHLVHQLLSLARNEVEVRLQPLDLATLVREVARQW
                     TPRALKAGIDLGFEGADHLLVLGEATLLREALSNLIDNALIYAGAGSEVTLRVQARGT
                     QVLVEVEDNGIGLSATDLPHVFERFWRASNLPGGCGLGLAIVNEIALRHGGSAAVLAA
                     EPHGLRVQLLLPRASSG"
BASE COUNT         2656 a         4314 c         4297 g         2588 t
ORIGIN      
        1 gctgctgctc tggtcatgac cggactggcg ctgaacctgc tgtggccctg gtgggtgcgc
       61 tggcgcaaac aacactgaag ctttttattg agttgattgc catgaccccc gtcgaactcg
      121 ataccgttga cctgcagttg ctcaaacacc tgcaaattga ttcatcgcaa agcaaccagg
      181 cgctggccga gctggtgcac gtgtcgccgc cgacctgcct gcgccgcgtc aagcgcttgc
      241 gcgacgctgg cctgatcgag cgtgaaattg cggtgctcag cgttgacaaa atcgccagcg
      301 tgattggcca cggcctgtgt gccgtggtgg aaatcagcct ggaccgccag gatcaagccg
      361 ccctgctggc gtttgaggcg cgggtgctgg cagaagacgc ggtgcagcag tgttaccggg
      421 tctcgcccgg gcctgacttt tgcttggtgg tgcatgcggc agacatgcca gattacctgg
      481 ccctgaccca gcggctgttc acgcaggatg ccaatgtgcg caatgtgaag accttcttca
      541 gcatcaagcg cagcaaattt ggcgcgcgcc tgccgttgcc gatgtcagtc tgagtttgcc
      601 cggctggcca ccccacagga aagccgcatc gccaggtgcc ttgctggtcc gattcttata
      661 tgccgtttat gcctgcagcc cttatgtatc aagcatcggt agctattcat ttagtagtgt
      721 caaagcaagc gcctgtgtgc gccgcaaagc agcctgcaag tcgcggcaat ttgaatgcaa
      781 tttttgcctg tagcccttat aggtattgcg ctgagcgcta ttgacttgat atcaatcgag
      841 ataccctgaa aacccttggt caggctttag tctccaacaa gtcaagtctt tgcataccat
      901 gcgaagcatt caaccttgat tcctcagttg gacgcgcccg agtgggccgc tggcggcgcg
      961 tctgggtagg cgcgacgacg acgcgggctc ctgcccgcta ggaggagtaa cgaccccagg
     1021 cgcgttgcca gtggctcaat cgggtgcgta gcgctgtcac ccaattggtg aactccatcg
     1081 aagctgaaaa agtcaatcgt cataagaaat tacagatgaa aacaagcggt caactgagga
     1141 atctaggttc aaacattcca agtcattcgt cgcgagggcg cgactcctac aaattcaaat
     1201 aggcgccgac tgtttgtgta ggagtcgcgc cctcgcaacg aaggccggcc aattccggct
     1261 tgtccagctc aggaaggtac cccgccatcg gccatcggca gggtcagact gacctggcag
     1321 ccgccatcgg ctacagcagc gatacggatg ctgccgccca tgcggtccat catcttgcgg
     1381 cattgcacca gccccagccc gagcccttca aattcgcgca ccgggtgcag ccgggcaaag
     1441 accttgaaca actgcccagc ctgccggggc aaaaaaccca cgccgttgtc ctgcaccgtc
     1501 aaggcacaca agccaccggc cagcggcgcc catgacacac tgatctgggc cggctggcgc
     1561 gccttggtga acttgcaggc gttttccagc agattcgcta tcacttggcg cagcaggctt
     1621 gcatcggcca tcagcatggg caaatctggt gctatctgcc actgcagcgc gcgctgcggg
     1681 tacttttgca ccagttcatc gatcacttct gccaccaaca aggcgctgga cgttggctgc
     1741 aaatgcacgg cttgcaggcc cagccgcgac aggctggcca ggccgtcaag ctgctgcgtc
     1801 agcaaatgcg ccgactgccg gatggtggcc aaatgcccgg caatgtcgac gggcaggtcc
     1861 ggcaggtctt cttcgatgat caaggcaaag gcgctgatgt ggcgcagcgg cgcgcgcagg
     1921 tcatgtgaca cggcgtaggt gaattcctgc aactcggcac gtgcctggtc aagctggcgc
     1981 tgcaaactct ccagcgtcgc atcgcgcccg cccgctggca cagccgccgc cgtgacatcg
     2041 tgcgccgctg ttggcacagc cgccgcggtg acatccgggc cggacgggcc ttgtgccgaa
     2101 ctcatttttt actcggccgc ttccagttgg caatgctgac ctggcgcgcg cgcgccacgc
     2161 tcagggccaa cggcggcgtg tctttggtca gggtggttcc gccgccgacc gtgccgccag
     2221 cgccaatcgt gaccggcgca atcagcacgc agttgctgcc aatgtgcaca tcggcctcga
     2281 tgatggtgcg gtgcttgttg gcaccgtcgt agttggcagt gatgctgcca gccccatagt
     2341 tgacgcgctc acccaccgtg gcatcgccca gataggccag gtggttggcc ttggcacctt
     2401 tggccagggt ggagttcttg acctcgacaa aattgccaat atgcacaccg ccaccgagtt
     2461 tggcccccgg gcgcaagcgg gcaaatgggc caatcagcgc gccagcgccg acttccaccg
     2521 cgtccttgct gcccggtgtg ccgccttcga tatggctgaa cgggtgaatc accgccccgg
     2581 ctgcaatgct ggcatttttg atgacacagt tggcccccac ctgcacccct tcatccagag
     2641 taacctggcc ttcaaacacg cagttgacat caatcaccac gtcttgcccg cacttcaggg
     2701 tgccacgcac atccagccgc gccgggtcaa tcaggcgcac gccttgctcc atcaactgcg
     2761 cagcgatgcg acgctggcag gcgcgctcca gttgcgccaa ctgcaccggg ctgttgatgc
     2821 cggccacctg cgttgcatcg ctggttttgt gggccaccac cgcaacccca tcggccaccg
     2881 cgaacttgac gacatcggtc aggtagtact cgccctgggc gtttttgttg tccagccggg
     2941 ccagccaggg cttgagcagc cgggccggca ccgccatgat gccgctatag acctcagaga
     3001 tcaggcgctg cgctggcgtg gcatcttttt gctcaacaat cgcctgcacc acttcgccct
     3061 ggcgcacgat gcggccatag ccgccggggt tggcaaggtc aatcgtcaaa agggccagcc
     3121 gctggccagc gcaggcggca atcagttgtg ccagcgtatc aggctgggtc agcggcacgt
     3181 cgcctgacag caccaccacg acaccgtcgt cctgcatgac cggcaccgcc tgctgcagtg
     3241 cgtggccggt tccaagttgt ggctcctggc gcacaaaatt caagtgaaat tggcttgtag
     3301 cccccgtgga ttgtgcgatg gcagcttcta cttctgtagc accatggcca gtgataacgg
     3361 tcacctgacg ggctgccagg gcaccggcca ggtccaccac atgttgcagc aaagcacgcc
     3421 cggccaaaag atgcaacact ttgggcagtt tgctcttcat gcgcgtgccc ttgcccgccg
     3481 ccatgatcac cacgtccacc ggcatcgccg gaaaaatatc cagattgctt gtcatgcgaa
     3541 cttctttctc ttgtcaattc atgtcttggc gcattatcag cgcgctgcta accctgctgg
     3601 gcgcgctgtt gctgggtggc tgcagtgttg cccggctggg ttacagccag gcacccgatt
     3661 tggtctattg gtgggttgac agctacctgg acctgagcga tgcacaaagc agccgcctgc
     3721 gcgccgacct ggtggccctg caccaatggc atcgcagcac tgaattgccg ctgctggcgg
     3781 gttcgctggc cgatctgcaa ggcgtggcgt tgcaggacac cacgccggtg cgtctgtgcc
     3841 agctcgtcga tgcgctgcgc ccgcgcctgc aagcggtgct cgatcaggcc gagcctggtt
     3901 ttgccacgct ggccgtcagc ctgaaacccg agcaactggc ccacttgcag cggcaactgg
     3961 aaaagcgcca gcaaaagtgg cgcaaggact ggctcgacgg cagtccgacc gagcgcctgg
     4021 agcgccgcac cgagcgcctg attgatcgca gcgaaatgtt ctacggtcgc ctcgacgcaa
     4081 gccaacgtgc actgctgcgt gcccgcgtcg cggcatcagg ctttgactcg acattggccg
     4141 aggccgacat gctgcgccgc cagcaggacc tgctgcaaac cctgcaagca atcggccgca
     4201 gtggcttggg cacgccacgc gcccaagatg atattcacca actgctggcg cgcctgctgc
     4261 tgccagccca gcctgccgag cgggcccgcc aagagctact gaggcaggaa aactgcggca
     4321 cctttgccgc attgcacaac agcaccagcc cggcccagcg caggcaattg gtcgaaacgc
     4381 tgaaggcttt tgaatccgac gcacgcgctt tgatgcctgg cacttcgcct cctaaaatgg
     4441 tcagcatcaa ccacaggaat tgaaatgtcg ttaaggttca ttgccgcagt cacccgcctg
     4501 attcaggccg atgagcaact tttggggggc cgtgtcacga gcgtgcagca cgccgccctg
     4561 cgcgaagccg cgctgctcaa ggcactgaaa gagttggcaa tgcaaagcgg ggtcgaactg
     4621 gaactgtcaa tgagcaagga cggcaaaggc gacatcacct tggttgccaa acctgaaccg
     4681 ggtcagtcag ccaagccagg tccggggcat ttcggccagg catttgtgga ccggctgaac
     4741 gctttcttgc cagtcacagg tcgccgcccc aatgcgatgc tgctgcccga gcgcggcaaa
     4801 tgccagatga accatctcga tgctgaaaag atggtcatgg catgctttga cgagttgaag
     4861 cgccagcagc cctgatcagt cacgcgacac cgcaggcacg ggcagcactg gaatgcgtcg
     4921 gcgcttgatg atgaccaccg gtggcggtcc ttcgcggggc cgagggggct ggtagaccgg
     4981 cagctcatcg ggtatttttg cattcgccag taaatcggca agttgttttt ggtccgactt
     5041 gcccaaggcg gtgatgtaat cgccaacgac cagcaccagt tcatcttcaa gcttggccaa
     5101 ggcaccgtac tgatgcccat ggcgggcact gcccaacagc ttcagcggat catctgctgg
     5161 tcgctggtag gtaaattttg accattcggg ctgaccaagc cgaatgctcc aatgagactt
     5221 tttcgccaat ttatggattt ccatgactga taagggttgc gccactgtaa tccgaaatca
     5281 ttctgattca ggacaaacaa ggcagcattt tagaaacagg tgatgatcgc atcgaccacc
     5341 gtgtaacgct ggtccacgat gggaatccag cgccatttgt caaaggtggt gcaggggtgc
     5401 gaaatgccca ggcagacacg gtcgcccact tgcagcgccg cgtgtgccgg gccagtgccg
     5461 gtcaggtaag catgctggtc gttgagtttg ctgatgcgcc agtcaggtgc tgcggcttgc
     5521 ggctggcaac tgccgtgccg acacaacagc agtggcgtgg gcatttccca gtcaaaagaa
     5581 atgtcgcgct tgcccaaacc cagaatcgcc agccccggct cggggcaaga ttgcacgctg
     5641 gcccaaactt ccatggcacc ctgcagacca gtgccacaac cggtgcgatc agccagcgca
     5701 ctcatcatgc gcttgtaggt accctgatcg tgggtaacgt agcaacctga gcgcagcaag
     5761 ccggtcaccg ggcggctcaa gctcagccgc aggccaccga caaccaggtc aaaaatggcg
     5821 gagccgccgg ccgtcagcag cacctcatcc gcgtcaaaca gattgcgcgc gtcgcacgcc
     5881 cgcgccaccg cctgcagcag ttccatcagc ttgtggacgt aggcttcatc ttgcgcactt
     5941 tgtccagtgg cagccaggcc ctcgtagcat gccaggccca ccagttgcac ggcggagctg
     6001 gcatgcagcc actcggccag ggtcagcgcc tgctcagaag tgcggcaacc ggtgcggcca
     6061 cccaccacac cgatttccag cagcacctca aacgccagcg cggccgggcg atgcgcccgc
     6121 caggcttcga tcaattcaag ttgcgccacc gagtccacca gaaacagcag ccgcagctca
     6181 gggtgcaagc gcttcaatgc gtcaatgcct gccaggtcca cctcggcaaa cacctggttg
     6241 gctatcagga tgcgggtggc accggccgcc acgccaatgc gtgcctgtgt cagcgtggca
     6301 aatgtcatgc cccaggcacc cgcgtccaac tggtgacgaa acaactgcgg cgacatgctg
     6361 gttttgccgt gcggggccag ccccaggccg tgcgatgttg caaagtcctg catccagcgc
     6421 aggttgtgct gcagttcatc gcgtttgatc accgccagcg gcagcggcaa atcaccaagc
     6481 aacacattcc agccctgcgc ggcaatgtgc gagcgatgca gcggcgcact gtgatgcgga
     6541 aagcctttga agctgctgtc gatcaatggg tcaagcatgg aagccttggt tgccatgacc
     6601 cgcctgagca agcctggctg tggtgatgca cgggatgctg tggtttcaat tacaccggcc
     6661 agaccacgcc atggtcgttg aggatggcat caagcgccat gtcatgcggc tcgggctcaa
     6721 agtcgggcaa aaagtcggtg gcaaaaccca gccctacggt aaatggccgg ggtgacagac
     6781 cggccagggt gcgatcataa aagccgccac catagcccag ccggtagccg cccggcgcat
     6841 agccgacaca ggccacgaac agcaaggtcg gcgtgatcag ttcggtgtcc ttgggtttgg
     6901 gaatgccaaa agcgtcatct tccattggac aacccggata ccaggcatga aaactcatgg
     6961 tcttgtgaac tttgtccacc accggcagtc ctatcttgcg caacctgggg cgatcaacca
     7021 gttcgccgtc ttccttccag cggtgcaagg cgggcaacgg gtcaaattca cctttgatcg
     7081 gccagtaggc accgatgacc gtatccggcc ggccgaccag ccagatgcgc atgacgcgct
     7141 gcagcaaatc cacccgctgc tgccggtcgg gcaggctcag tcggcgatcg accagggttt
     7201 tacgcagggc tcttttatca gatgcccttt tttcatcgga gttattcata aaatccatct
     7261 atgcagttca caaacatttt gacattgatt agcctcacag gcgcaatggt ggcgctgcca
     7321 gccaccgcac cagccgccag ccgggccgac gacgtgctgc tggagatgaa cagcgctttc
     7381 cagaagggcc aaggcaaacg tctgaccgag ttgctgccac aggccaaagg ccacattctg
     7441 gaaccctggg ccgcctactg ggagttgcgg gcccgcttgg acaatgccag cgccgatgaa
     7501 gttgaaggtt ttttgagccg ttatcgcggc agctaccagg aagaccgcct gcgcaacgac
     7561 tggctgctgc tgttgggcca gcgccgcgac tggggtcgct ttgcgtcaat agcgcccgac
     7621 ttccggatga acgatgaccg tgaggtgcgc tgctacacgc tggcaatgga gcagattctg
     7681 gccggcgtgg atttgtccga agatgtcaaa acccagtggt accggcaaaa agaaggcggt
     7741 gcggcatgtg ccctggcggc tgaaaaacac cacgcggcca aaaaattgac cgatgccgac
     7801 atctggcgca aggcccgtct ggcggttgag gcacaccagt tcaagtctgc gcgccaggcg
     7861 gtgttgatcg tcgcacccga tgccgatggc gcactgagcg aactggcagc cagcccagag
     7921 cgtttcctgt cgcgcctgac cccgtcagcc aaacctcaag cccaggagct ggtggtgttg
     7981 gccttgatcc gcttggccga cagcgcgccc gaccgggccg cagtcgagct ggaaaagcgc
     8041 tggggccggt atctgaccgg cccgcagcgc gcctgggtct ggggcagcat tggcaaacag
     8101 gccgcgcaaa agctctctga cgacgcgctc ggctatttca gtcacgccag tccggccgac
     8161 atgagcgacg aacatctggc ttggcgcgtg cgcgcggcgc tgcggcaaaa ccagtggcat
     8221 gatgccttga aggcgatgca agcgatgagc gccgacactg ccgatgacgc ggcctgggtc
     8281 tattggcgcg cacgggccat gctgcaaccc ggccgcaccg atgccgagca agtgcaggcg
     8341 cgccagttgc tgcaaagcat tgccgggctg ggtggctttt acgaaatcct ggcgcaagaa
     8401 gagttggggt tgccaattgc gccacctgcc acgcctgagc cgctgagcac cgctgaaaaa
     8461 gacaacgccc gcgccaaccc cggcttgcaa cgtgcgctgg cagccgtcgc cctggggctg
     8521 cgcgcagaag gcgtgcgtga atggaactac agcaccaatt tgcatgcgcc gggcggcatg
     8581 aacgaccgcg aactgctggc agcggcagac ctggcctgtc aacacgaggt gtgggaccgc
     8641 tgcatcaaca ccagtgaccg cacccggtcg gtgattgacc tgaatcagcg ctttccgatg
     8701 ccgcacaaaa aggccgtgct ggcgcgcacc cgcgatatcg gactcgaccc ggcctacgtg
     8761 tatgggctga ttcgtcagga aagccgcttc atcatggacg cacgctctgg cgtgggggca
     8821 tccggcttga tgcaggtgat gcccgccacg gccaagtgga cggccaagaa aattggcatg
     8881 aacgacttca aggtgcagca gattaccgac cgtgacacca acattgccat cggcaccggt
     8941 tacctcaagc tggtgcttga tgactttgcc gggtccatgc cgatggccgc cgcggcctat
     9001 aacgccgggc cgggacgacc gcgcagttgg cgtggcaaca gcggcgcacc ggccatcgag
     9061 gccgccatct gggccgagaa cattcctttc aatgaaaccc gcgactacgt caagaaagtg
     9121 ctcgccaaca ccaccattta cgcggccatg atcagcggga aagcccagtc gctgaaagcg
     9181 cggctcgggc tggttggtcc gcgcgagatc aaagtcccgg aaaaccgcga cctgccctga
     9241 aaccctgtga accgtcattg ccgcccacgc ggcaaatttt ggcaacttca gattctcgcc
     9301 tgcgcgccag ttcaaccctt cgcttcacct tttccacgct gctattgcca tgctcaaact
     9361 ctacattggt aacaaaaatt attcttcctg gtcaatgcgc ccctgggtgc tgctgaaaca
     9421 ggccgagatt gaatttgaag aagtcatggt tcgttttgat gccttcacgc ctgactcgca
     9481 attcaagacc accatcagca agatcaaccc ggtggccaag gtgccggtgc tggttgacgg
     9541 cgtgctggtg gtgtgggaca cgctggcgat tgccgaatat ttggccgagc agtttccgca
     9601 aaagcagctt tggcccgccg accgggcagc gcgagcccgc gcccgcagcc tgtgtgccga
     9661 gatgcacagt ggcttttccg cgctgcgcag tgcctgcccg atgaacatcg aggccagcct
     9721 ggcgcaggtg ggcgcgctga tctggcgtga ccagcccgcc gtgcgcgccg atgtggcgcg
     9781 gctggtggcc atgtggcagg cgctgctggg cgaacacggc ggcccgctgc tgtttggtga
     9841 cttttcggtg gctgatgcct actttgcgcc gatttgcacc cgactgatca cttacgcgct
     9901 gccgctgccg cctgacctgg cctcctatgc ggcgcgggtt tgcgcgctgc ccggtgtcaa
     9961 ggcctggatt gaccaagccc gggccgaaca tgactttctg gactttgaag agccctaccg
    10021 cctgaaaccc tgacatgcaa atctacctgg tgggcggtgc cgtgcgtgat gccttgatgg
    10081 gcttgcctgt caaggaccgc gactgggtgg tggtggggtc cacgcccgat gagctgatca
    10141 gccatggctt tgtcccggtt ggcaaggact ttccggtgtt tttgcatcct cgcacccatg
    10201 aagaatttgc gctggcccgc actgagcgca aaaccgctcc ggggtaccac ggctttgccg
    10261 ttcatgcggc cccggacgtg acgctggaag aggatctggc aagacgcgac atcactatta
    10321 actcaatagc tgtcagcgca tacagcataa gggatagcgg ccaatttgat gtcaaggttt
    10381 caggtgaacc cgaaacctgg ctgggcactg gcgcactggt tgatccacat ggcggtgtgc
    10441 aagacctggc gcgcaaggtg tttcgccatg tcagcccggc gtttcgcgaa gacccggtgc
    10501 gcatcctgcg ggtggcgcgc ctggcggcgc gctttgccga cttcacgctc gcaccggaaa
    10561 ccctgacgct gatgcgagcg atggcagaag acggcgaagt cgatcacctg gtggccgaac
    10621 gggtctggca ggagctggcc aggggcttga tggaaaacac cccgtcgcgc atgttcgagg
    10681 tgttgcgcga ctgcggggcg ttggtgcgcc tgctgcccga agttgatcgg ctgtggggtg
    10741 tgccacagcg agccgactac caccctgaag ttgataccgg cgtgcacctg ctgctggtac
    10801 tcaacatgtg tgcccggttg caggcatcgc tgccggtacg ggtggcctgc ctgtttcacg
    10861 acctgggcaa aggcaacacc ccgcctgatg tgctgccgcg ccacatcggc cacgaagagc
    10921 gcagcgcccg gctgctcaag gtggtatgtg agcgcctgcg cctgcccacc gagtgccgcg
    10981 aactggctga tgtggtggcg cgtgagcaca gcaacatcca ccgcagcgac gacctcggtg
    11041 ccagcgcgct ggtgcgcctg ctggagcgct gcgatgcgct gcgcaagccg cagcggtttg
    11101 ccgagctgct gctggcctgc gaatgcgacg tgcgcggtcg ccatcaacac agcgagcaag
    11161 cctatccaca acgccagcgt ctgctcaggg tgctggcggt gatgcagcag attgatacaa
    11221 aaagcatagc tgccaaggca atcacggcgg gggctacagg cctgaaagta ggtgaactga
    11281 tacacaaggc caggattgct gcggtgcagg ccaactgtca ggatctgcag ccagtgctca
    11341 gcagttgagc tggtctggcc tgactttctg ttcatcccgg attcctcaat tggacgcgtc
    11401 cgagtgggct gctggcggcg cgtctgggta ggcacgacga cgacgcggct cctgcccgca
    11461 tgtcgttctg gcggacgagg caggcgcaac gccgccagcc gcgttgccag tggttcaatc
    11521 gggtgcgtgg cgcggtcaac agagaaatcc aggatcaaca gcgcccttgc aactcggcgc
    11581 atcccaggcc cggcgggttg gcagtggcca tggcgcaggc tgggcaatgc gctagggtat
    11641 gcttgcagca tgtcaagttt gctcattatt gaagacgatg aattgctgcg cgatggcctg
    11701 tgcgcccaac tggcgcaggc cggccacagt gtgacagcgg cggccgatgg cgaggccgcg
    11761 caagcgctgc tggagaagcg ccgctttgat ggcgtggtac tggacctggg actgcccaag
    11821 atcagcggca tggacttgct gcattgggtt cgcaaacgcc tgccggcgct gccggtgctg
    11881 attctgacgg cgcgctgcgg tgttgatgac cgggtccagg gcctcaatgc cggtgccgat
    11941 gactacttga ccaaaccgtt tgaaatggcc gagttgcagg cgcgcctggg tgcgctgttg
    12001 cgccgcgccc gcctgcctgc ctttggcggc acgctggagg tcgccagcaa caccacccgt
    12061 cgcctgcgtg tcgatgccag tttgccgcaa gcctggctgg gtgacgaagc gctggaactg
    12121 acccagcgcg agtgggcctt gctgtcgctg ctggtgaccc aagccgggcg ggtggtcagc
    12181 cgcgaagatg tgctggaaac ctggtctgcc gaaccgggcg aaggcggcgg cgctggtgtc
    12241 gcctccaacg cgctggaggt gtacatccac cggctgcgcc gcaaactggt cgattcgggg
    12301 ctgaacattc gcaacgtgcg aggcctgggc tacatgctgg aatccaccgc agcatgaaca
    12361 gaccgggcgc agacgcagcc gccgccgcgc ccgcctcgat ggccagtgtc gccgcacccc
    12421 ggcaaggcct gtcgctcaag cgccagttgc tgctgtggct gctggccccg caactggtgc
    12481 tgtttctggt cggcggtgcg ctggcttacc gcattgcctt gagttatgcc gaaaaggcca
    12541 ttgaccaatc gctcacccag tcggtgcgat cactggcgcg ccaggtcaag ccggtgggct
    12601 cggggctgtt gattgacttt ccacgcgccg cgcaagacat catcgagcaa gacccaaaag
    12661 accgggtcag ctacatggtg tcgtcgccgc cagggcactt tttgctgggc aacggcaagc
    12721 tgcccgaccc gattgggtca gcacccctgc tgaccaacga agccgtgttg tacaacaccc
    12781 gggtagacgg caaggccatg cgggtggcct tgctggaggt ggactatggc gatgcccagt
    12841 cgccgcagcg catgcgggtg caggtggcca aaggtctggc ggtgcagcag cgtctgacca
    12901 ccgaactgat tgccgacatg ctggcaccgc tgctgctgct cggtgcattg ctgagcatgc
    12961 tggtgtatgg cggcatctcg cgtggcctgc agcccctgac ccgactgcag gcgcagcttg
    13021 gtgaccgcca gcttgccgac ttgtcggcgc tgtcaccgat cgagatgacc caggcaccac
    13081 atgaagtgca tgccctggca gcagcggtga accagttgct gtcagcggtg cgccgcagcc
    13141 tggggcagga aaagcgattc ttgagcgatg ccgctcacca gttgcgcacg ccgctggcag
    13201 gcctgatcag ccagaccgag ctggcgctga ccgagtccga cccggccgcc ttgcgcgagc
    13261 gtctgaccaa ggtgctggcc ggtgcccagc gcagcgccca tctggtgcac caactgctgt
    13321 cgctggcacg caatgaagtc gaagtgcggc tgcagccgct ggacctggca acgctggtgc
    13381 gcgaggtggc gcgccagtgg acaccgcgcg ccctcaaagc cggcattgat cttggttttg
    13441 aaggggccga ccacctgctg gtgctgggtg aggcaacgtt gctgcgcgaa gccctgagca
    13501 atctgatcga caacgcgctg atctacgccg gtgctggcag cgaagttacc ttgcgggtgc
    13561 aggcgcgtgg cacgcaggtg ctggtggagg tagaagacaa cggcattggc ttgtcggcga
    13621 ctgacctgcc acatgtgttc gagcgtttct ggcgcgccag caacctgccg ggcggctgtg
    13681 gtctggggct ggcaatcgtc aacgaaattg cactacgcca cggcggcagc gcagcagtgc
    13741 tggccgccga gccacatggc ttgcgtgtgc aattgctgct gccgcgcgcc agtagcggct
    13801 aacggctaac ggcgagcacg ttaagccagc cgcaagccag tctgcgcatc aaacc
//
LOCUS       DAOSLL010000041        13722 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4570_length_13722_cov_3.923319, whole genome shotgun sequence.
ACCESSION   DAOSLL010000041 DAOSLL010000000
VERSION     DAOSLL010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 13722)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13722)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13722
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4570_length_13722_cov_3.923319"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PLB25_03005"
     CDS             <1..747
                     /locus_tag="PLB25_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE40594.1"
                     /translation="IRSIPTCILMMGGKPVDGFTGAQSAAQVRTLLDKHLPSQGELIA
                     EAEVDEAQELLQAGDLHAALAKLADALAADPANDDTRYDYIRLLIELGGLEEAEAALA
                     PKLAEIPRKLRFEALAQWLSAINFIAGSAYADWSIAQFDEKIGQNKRDFDTRFAKARL
                     LMAAGEWTAAMDELLEIIMRDKKWQDEAPRKAFVAILELLTPAKPKAAAQISGKTAGG
                     IEVLGKAVVQQDPQAEMVSAYRRKLSMMLN"
     gene            complement(837..1604)
                     /locus_tag="PLB25_03010"
     CDS             complement(837..1604)
                     /locus_tag="PLB25_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011660128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE40595.1"
                     /translation="MGERLANRVALISGGATGVGGAAARLFAAEGARVVIVDINGPEA
                     ERTAQQVRAAGGQAMVVVADVSQATAVEAAVARVVQQWGTVDVLFNHAGSIVVKPFLD
                     TTEEDWDRLMAINVKSMYLVTRAVLPHMLTRGRGAIVCTSSISAVAGTPGEVLYDTTK
                     GACHMFARAIAVEFRDRGIRCNAVCPGFIRTPHGLRELDALQKMGVDVSDAAMAAAQG
                     RICEPEEVAAVALFLASDEASFVNGAQLFVDNGFTAV"
     gene            complement(1597..3132)
                     /locus_tag="PLB25_03015"
     CDS             complement(1597..3132)
                     /locus_tag="PLB25_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HOE40596.1"
                     /translation="MSIEPHKTAEADLKLLHSMGYAQELARNMKGFQNFAISFSIICI
                     LSGGINSLGQGISGVGGAAIGIGWLLGCAVSLVFALGMAQIGSAYPTAGGLYHWSSIL
                     GGRAYGWLTAWLNLLGLVTVLGAINVGTFNFFVGAFGPMLGLEANFATQATFVIGITV
                     VQALCNHVGIRFTARLTDLSGYLIFAVALALTVSLLAYAPSIDVSRLWTFKNYSGPAG
                     GDVWPATGNMLMLFGLGLLLPIYTITGFDASAHTAEETHDAARAVPRGIVNSVLWSAL
                     AGWIMLSAFVLAIPDMDKAAASGWSVFFVTLDAVLPVSLKVILYALIFVCQLLCGLAT
                     VTSASRMIFAFARDGGLPASSALKRVHASFRTPVAAIWTAAAVSIAFTLYTPAYATIV
                     SVTVIFIFLSYGLPVIAGLFAYQRSWTRMGPWDMGPMYRVVGVLAIAAIALVFYLGVQ
                     PPNDAALWITLVFLAATALIWFGFERRRFKGPPMGDEVARLQAEIAAAEKALAAAEQA
                     KNG"
     gene            3774..3849
                     /locus_tag="PLB25_03020"
     tRNA            3774..3849
                     /locus_tag="PLB25_03020"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3807..3809,aa:Thr,seq:cgt)
     gene            4043..5278
                     /locus_tag="PLB25_03025"
     CDS             4043..5278
                     /locus_tag="PLB25_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HOE40597.1"
                     /translation="MLTDAQCRNATCPPDKTRGRFSDAGGLYLEVSPAGSKRWFLKYR
                     AIGKEKRLALGSYPDVSLTAARKARDAAKLQKSEGVDPVQARQVEKLKISVGSGDTLA
                     ATASDWLERGKPNWSATHYVRENRNITKDLIPYLGKRDIASIKPIELLAVIQKVEERG
                     ALDVAHRVLITAHGVWCYAVATGRAERDITPDIKKALKPHIKENLPAITDPMKLGELL
                     RASEAYNGGPVVRAALKLAPILFQRPGNLRTMRWADLNLDAALWSIPSEDMKRTKAEK
                     INGQAHVVPLPRQALEVLRDIQKLTGDREYVFPGLRDWRKPMSEAGISAALHAMGYKD
                     IHTWHGYRATGRTILRQVLKYPKDVIEAQLAHTGQITHGGAYDRATHTDERADMLQVW
                     ANYLDQLAKGADVIQFKAA"
     gene            5374..6045
                     /locus_tag="PLB25_03030"
     CDS             5374..6045
                     /locus_tag="PLB25_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40598.1"
                     /translation="MIGFGESVEINLDVKRFVNDDETRAVAIIVAKHAENRRIAYRLT
                     EKPGGRGMVRGKFYWSQTVPWYEWMSEPRILQSCSEECGLKIWQLAEVIAYQIRPSNW
                     QVFAWMSIALQSQNHEKAISDALLQSIEKIKRMRDQSQSDARTALAKRGAAAKIASDP
                     KQADKALVRQCWDDWQKHPDRYKGKSAFARDMLDKFPNLKSQRVICDKWCPAWEVENI
                     TLPAQ"
     gene            6175..6507
                     /locus_tag="PLB25_03035"
     CDS             6175..6507
                     /locus_tag="PLB25_03035"
                     /inference="COORDINATES: protein motif:HMM:NF017717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HOE40599.1"
                     /translation="MAILRMTGVLAETGLASHATVYGDIHAGLFTVPVQISERAVGWP
                     DTEVRAIIAARIRGDSEEQIRALVAKLHTARMAGSDEPFKTDWFDRSAQLKKCAAQRR
                     KRTTLATA"
     assembly_gap    6515..6524
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6902..7504
                     /locus_tag="PLB25_03040"
     CDS             6902..7504
                     /locus_tag="PLB25_03040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40600.1"
                     /translation="MRKLGEILRDTPKNQGAKGLPGGGTRGTKKEPRVDEAPTLDELG
                     LSKKESAVAQKLASLSDDAFEQVRSGSITIAKAVAVVAAEKAKPEPKLYPVNPDVQDV
                     EFRESEPEDTGPDAEELAAQEAAEQADRQTMQLLLDSDDKFATAVAEINRLKAEVFIL
                     KQSRNGYMNEVNAAKALVNKRDRLISKLNKEIAALKRGAA"
     gene            7501..9030
                     /locus_tag="PLB25_03045"
     CDS             7501..9030
                     /locus_tag="PLB25_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014111104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOE40601.1"
                     /translation="MNSKLWDDAPHYGSETFPPPRDFQTTAHEAIRQAYREGHSKICL
                     MAPTGGGKTYLGLRIAHEALVKGRKVVFLCDRTTLINQTSSAADSYGLSAHGIIQANH
                     WRRDKEMPFQIASAQTIQKRGYWPDADVIVVDECHTQMKVWTEFIQTCRTAVIGLSAT
                     PFSPGLGKLFTKLVNATTMHELTQAGVLVPMRVFSCKRPDMKGAATSGGEWTDQAAAN
                     RGMEIIGDVISDWQKFAENRKTIVFGATIAHCQELARQFVEAGVMAACFTSETTATER
                     AALLVEYTKPDATLRVLISVEALAKGFDVPTVGCVVDCRPLRKSLSTAIQMWGRGLRS
                     SPDTGKVDCLLLDHSGNIQRFADDFEEIFHHGLSALDMGEKLDKKVRQDDDEHEAKGC
                     PECGYKPFAKRCMACGYEKQAPSLVETLPGEMREVVLGKKKLADDHRHLWAQVCTYAR
                     SHSKPEKQRGRAWNLFRNITGQAPSNLWSFETTPNVEITRNVRNKITSLNVAYSKARA
                     I"
     gene            9027..9905
                     /locus_tag="PLB25_03050"
     CDS             9027..9905
                     /locus_tag="PLB25_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HOE40602.1"
                     /translation="MTFIDFARAHGVDIDPAKLYAGERIRRCGTTDKPRSANGAYFWD
                     GQRGWVFNWASEARVQWFNDQDAQPWTDAEKQAWKARRQAMQAHQDDQHHRAAARAAE
                     LIRSTKPGTHDYLARKGFPDLEGMIDSTGTLVVPMRHLETNALLGAQLIHWDEPERQW
                     VKKMLPGMKAKGAILRLGDKTASETFLVEGYATGLSVLAALRSVGLRACVVVCFSANN
                     LEFIAPQVKGRVFVFADNDKSGVGEKAAMATGKPYCMSPVEGEDANDMHDRAGLVPVV
                     QLLMNVRLGGAHGERS"
     gene            9952..10608
                     /locus_tag="PLB25_03055"
     CDS             9952..10608
                     /locus_tag="PLB25_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40603.1"
                     /translation="MAIPWSVLDCPAYARLSHPARSLLLEIARQFVRDNNGRLLTSLR
                     HLESRGWKSWDVITRAKRELLESGFIHETVMGHRPNKASWYALTWRALDKIPGFDPGA
                     EASFERGAYMKKIDVLTPSHGAGMPVIAPPDGAGDPPTAPPDGAIRAVLPLPPAPSHG
                     DHLEKPSTVVCLSAGIPDVRLPASLTLIGRVLHRARPANRPAGYAVARSRFPFGVNHD
                     "
     gene            10601..11215
                     /locus_tag="PLB25_03060"
     CDS             10601..11215
                     /locus_tag="PLB25_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40604.1"
                     /translation="MIRRFIDPDLLPLTSDQKNILLHLVAMRYSARKFIASQNINRQA
                     IAILESAHREAIECGDTEGEALAKIRLDRAKEIGFELRFKIIEVGKDFIAASGHYDQL
                     PREVWLRALSVNESEWGSPDMRKYGDTIRNVVNVLDLENSATRDSAIINKPLKWCCTM
                     GLMHTSQTNPALGRVMHDACNEMFGGAFGEYVPPTMLEQMGVRP"
     gene            11272..11802
                     /locus_tag="PLB25_03065"
     CDS             11272..11802
                     /locus_tag="PLB25_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40605.1"
                     /translation="MLPNTLKIGPFGAKPKSPDEIAALNLDNAARNLPDECFANDPAV
                     IVAAADVVAWRNRLDRLASYPSKRNAILILISDIEAELAQAKEACRLACLDSFLADDH
                     DFIGALAVQERVALLENRLQAARTAKIDIERSYSEMTQLHALADKAEANLHNTRFALK
                     RQSLSDNAPKETRHGN"
     gene            11792..12217
                     /locus_tag="PLB25_03070"
     CDS             11792..12217
                     /locus_tag="PLB25_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40606.1"
                     /translation="MATKKLPRTAWKPGESGNPSGRPAGSGRVHKWRAGLDADVPEIL
                     TALVKQAKAGDVMAARLILERALPALKPSEMAQTIDLKGDTLAAKGESVLVAVADGSL
                     AVSQGAQLLAAVGTLGRLVEIDELAARISKLEEKQNGKS"
     gene            12204..12434
                     /locus_tag="PLB25_03075"
     CDS             12204..12434
                     /locus_tag="PLB25_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40607.1"
                     /translation="MANLESRIEKLEQAMGQAQEAPNMTLQFIRPEDKFCCAYRRLKT
                     GEEVVQRDNESDADFVARAQAAGFERTHHEQS"
     gene            12421..12603
                     /locus_tag="PLB25_03080"
     CDS             12421..12603
                     /locus_tag="PLB25_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40608.1"
                     /translation="MSNLESRLTKLEAVQQTPRRQYTDTERAVRYAYLLAQGGPLADR
                     LRSLVEKVPEDDHAKP"
     gene            12590..12799
                     /locus_tag="PLB25_03085"
     CDS             12590..12799
                     /locus_tag="PLB25_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40609.1"
                     /translation="MQNLEHRVAKLEADLLKVRFDELSDDELRDYAYSLPFQSPRQYK
                     ALIALILRHPSTIPIVHDDPAYLER"
     gene            12802..13215
                     /locus_tag="PLB25_03090"
     CDS             12802..13215
                     /locus_tag="PLB25_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40610.1"
                     /translation="MVRWNKLQLREESRCKGIVPGAATEEAPIFLGASPFLAAPRFVR
                     DAPTITMNDNPLPNRHRYAAEIEGYRPSITRRILLALHLVKPVKGTSKANWHYSEVDT
                     EGEFFCVLCRGPDGQNISRPIYEPPKFPPRRIKHI"
BASE COUNT         3290 a         3868 c         3950 g         2604 t
ORIGIN      
        1 atccgcagca tcccgacctg cattttgatg atgggcggca agccggtgga cggttttacc
       61 ggtgcccaaa gcgccgcgca agtgcgcacc ctgctggaca agcacctgcc cagccagggc
      121 gaactgatcg ccgaagcgga agtcgatgag gcgcaagagt tgctgcaagc cggcgatctg
      181 cacgcggcgc tggccaagct ggccgacgcg ctggcagccg acccggccaa tgacgacaca
      241 cgctacgact acatccggct gctgatcgaa ctcgggggtc tcgaagaggc cgaggcagcg
      301 ttggccccga aactggcgga gattccgcgc aagctgcgct tcgaggcgct ggcgcagtgg
      361 ctaagtgcta ttaattttat agctggtagc gcttatgctg attggtctat tgcgcaattt
      421 gatgaaaaaa ttgggcagaa caagcgcgac tttgacacgc gctttgccaa agcccgtctg
      481 ctgatggcgg cgggggaatg gacggcggcc atggacgagt tgctggagat catcatgcgc
      541 gacaaaaaat ggcaggacga agccccgcgc aaggcctttg tggcgattct ggaactgctc
      601 accccggcca aacccaaggc tgctgcgcaa atctctggca agaccgctgg cggcattgaa
      661 gtgctgggca aagccgtcgt gcaacaagac ccgcaggcgg agatggtgtc cgcctatcgg
      721 cgcaaactca gcatgatgct caactgacgc agtcaaacgc actgcaagcg tcaacgttga
      781 gcccgcctct ggcgcgggct gcgctaccag gcggcactgg gcggcacaag cgtagctcag
      841 acggcggtga acccgttgtc tacaaacagt tgggcaccgt tgacaaagct ggcttcgtcg
      901 ctggccagaa acagcgccac agcggccact tcttcgggtt cacagatgcg gccctgcgcg
      961 gcggccattg ccgcatctga cacgtccacc cccatctttt gcaaggcatc aagctcgcgc
     1021 aggccatgcg gcgtgcggat gaatcccggg cacaccgcgt tgcagcgaat accccgatca
     1081 cggaattcca cagcaatggc gcgggcaaac atgtgacaag cgcccttggt ggtgtcgtaa
     1141 agcacttcgc ccggcgtgcc tgccaccgcc gaaatcgacg atgtgcagac gatcgccccg
     1201 cgtccacggg tcagcatgtg cggcaacacg gcgcgcgtca ccaggtacat gcttttgacg
     1261 ttgatcgcca tcaggcggtc ccagtcttct tcggtggtgt cgagaaaagg cttcaccaca
     1321 atgctgcctg cgtggttaaa caagacatcc accgtgcccc actgttgcac cacccgggcc
     1381 actgcggcct ctaccgccgt ggcttgtgat acatcggcca ccaccaccat ggcctggcca
     1441 ccggcggcgc ggacctgctg cgcggtgcgc tcggcttctg gcccattgat gtccacaatg
     1501 accacccggg caccctctgc ggcaaacagc cgtgccgcag cgccccccac accggtggct
     1561 ccgcccgata tcagcgccac ccggttggcc agtcgttcac ccattttttg cctgctcggc
     1621 cgctgcaagg gctttttcgg cggcggcaat ttcagcctgc aggcgcgcca cttcatcacc
     1681 catcggcggg cccttgaagc ggcggcgctc aaagccaaac cagatcagcg cggtggcagc
     1741 cagaaacacc agcgtgatcc acagagcggc atcgttgggc ggctgcaccc ccagataaaa
     1801 caccagggcg atggcggcaa tcgccagcac gccgacgacc cggtacatcg ggcccatgtc
     1861 ccatggcccc atcctggtcc aactgcgctg gtaggcaaac agcccggcta tcaccggcaa
     1921 accataagac aggaaaatga agatcaccgt caccgacacg atggtggcgt aagccggcgt
     1981 gtagagcgtg aaggcaatcg acaccgcggc agcggtccag atggccgcca ccggtgtgcg
     2041 aaagctcgca tgcacccgtt tgagcgcgct cgacgcgggc aaaccgccgt cgcgcgcaaa
     2101 tgcaaagatc atgcgcgatg ccgaggtgac cgtcgccagg ccacagagca gttgacagac
     2161 aaagatcagc gcatacaaga tcactttcag tgacactggc agcactgcgt ccagcgtcac
     2221 aaaaaacacg ctccagccgc tggcagcagc cttgtccatg tcaggaatcg ccagcacgaa
     2281 ggccgacagc atgatccagc ccgccagggc cgaccacagt accgagttga caatgcctct
     2341 gggcacggcg cgggctgcgt cgtgggtttc ttcggcggtg tgcgccgacg catcaaaacc
     2401 ggtgatggtg tagatgggca acagcagacc cagaccaaac agcatcagca tgttgccagt
     2461 ggctggccag acatcaccgc cagccgggcc gctgtagttt ttgaaggtcc acaaacgcga
     2521 cacatcgatg gatggcgcgt atgccagcaa ggacaccgtc agtgccagcg ctacggcaaa
     2581 gatcaggtag ccagacaggt cggtcaggcg ggcggtgaat ctgataccga catggttgca
     2641 caatgcctgc accacggtga tgccaatgac aaaagtggcc tgcgtggcaa agttggcctc
     2701 cagccccagc atggggccaa aagcgccgac aaagaaattg aaagtgccga cattgatggc
     2761 cccaagcacc gtcaccagac cgagcaggtt aagccaggcg gtcagccagc cgtaggcacg
     2821 tccgcccagg attgagctcc agtggtacag cccgcccgca gtggggtagg ctgagccaat
     2881 ttgcgccatg cccagcgcaa acaccaggct taccgcgcag cccagcaacc agccaatgcc
     2941 aatcgccgcg ccgccaacac cgctgatgcc ctgcccgagc gagttgatgc cgcctgacag
     3001 gatgcagatg atcgaaaaag aaatggcaaa gttctgaaaa cccttcatgt tgcgtgccag
     3061 ctcctgggcg tagcccatgc tgtgcagcaa cttcaggtca gcctctgcgg ttttatgcgg
     3121 ttcaatggac attggatact ccagggtggt gggtcggtaa gacacatttg cggggccgca
     3181 tagtgccaac gacactctaa gcaagtttcg ttcctgccca tcttggtgcg ggccgcatcc
     3241 tcggcccaga tgatcgcgcc cggcttggcc agccagcgtc ggccatgctg tcggtccgtg
     3301 gtctgcgggg atcgtcctcc ttgcacacct tgcgtggctg gcagaacccg gcttgaccaa
     3361 gccgcgccgc ttcatggctg tcgaaaacat gatcgatggg cgggtttcac catagccttg
     3421 gtgctttccg cagccggttt gtcaggttag ccggcccagc attcaatgac ctccaccggg
     3481 aggggcggca cgacaccggc atcttgaggt cgcttcaaac gctctgcggc aaggggcaac
     3541 ggctggccaa tcgccgcctc gtgaaccctt catggcgagc gtgcgtctgc ctgcagtgcg
     3601 tgaagatgtt tccagccgat gctcgatacg cccggcctgc tcctggcaat tgaggccagc
     3661 cattggtgcg ccggtccggc gccaactgcc ccgcgcgtga attcgcaggg gctgatcggc
     3721 cttcgtggga aaagattgat aaactcaacc gccagtgctt ttgaagtact gtcgccgatg
     3781 tagctcagtt ggtagagcaa ctcactcgta acgagtaggt caccagttcg attccggtca
     3841 tcggcaccat cagatagacc aaaggccttt aaggagcgat tcttaaaggc ctttttcatt
     3901 ggtaaatcag ctagttacat tgcagtaagg tacaaaggcc gctattgaca ttcaaggcaa
     3961 agcgggggaa catacggggg aacgaaacac ggtggaacag cacttttacc cccgtgttcc
     4021 cccactccat aggagaaccg ccatgttgac tgatgcccaa tgccgtaatg cgacctgccc
     4081 gccagacaag acccgaggcc ggttttcaga cgctggcggc ttgtatttgg aggtaagccc
     4141 ggcgggatcg aagcgctggt ttctgaaata ccgggctatt ggcaaagaaa agcggctggc
     4201 gctgggcagt tacccggacg tgtcgttgac agcagcccgc aaagcccgcg atgcggcgaa
     4261 gttgcaaaag tctgaaggtg ttgatccagt acaagcacgg caagttgaaa agctcaagat
     4321 atccgtgggc agtggggaca ccctggcagc gacagcaagc gactggctag agcgtggtaa
     4381 gccaaattgg agcgctaccc actatgtccg cgaaaatcgt aacatcacca aggacttgat
     4441 cccgtatctt ggcaagcgtg acattgccag catcaagccg atagaactgc tggcagtcat
     4501 ccaaaaggtt gaagaacgcg gagcgctcga cgtggcgcac cgcgttttga taacggcaca
     4561 tggcgtgtgg tgctacgcgg tggcaacagg ccgggctgaa cgtgacatta ctcccgacat
     4621 caagaaagcc ctgaagccac acatcaagga aaacctgcca gccattaccg acccgatgaa
     4681 attgggcgaa ctgctacgtg caagcgaagc ctacaacggc gggccggtag tacgtgccgc
     4741 actgaagctg gcaccgatat tgtttcaacg accgggcaac ttgcgcacca tgcgctgggc
     4801 tgatctgaat ctggacgcgg cgctgtggtc aatccccagc gaggatatga agcgcaccaa
     4861 ggccgaaaag atcaacgggc aagcccatgt agtcccactt ccacggcagg cgctagaggt
     4921 attgcgtgat attcaaaagc tgacaggtga tcgggaatat gttttccccg gcttgcgtga
     4981 ctggcgcaaa ccaatgtctg aggctggcat cagcgctgca cttcacgcca tgggctacaa
     5041 agacattcat acttggcatg gctaccgggc gacagggcgc accatcttgc ggcaggtgct
     5101 gaaatacccc aaggacgtga tcgaagcgca gctagcccac acagggcaga ttacccatgg
     5161 cggcgcatac gaccgggcaa cgcacactga cgaacgggcc gatatgctgc aagtgtgggc
     5221 gaactacctg gaccagttgg ccaaaggcgc ggacgtgatc cagttcaagg cagcatagaa
     5281 gtttttggcc acgcctagcc cgacggggcg aaaagcagat tacccctgat ctgcctggcg
     5341 ctggttaatt ctcagggcgt ttagggggac gggatgatcg gttttggcga atctgttgaa
     5401 ataaacctag acgttaaacg gtttgtcaat gatgacgaaa caagggctgt agcgatcatt
     5461 gtcgcaaagc acgctgaaaa tcgacgaata gcctacaggc taactgaaaa gccgggcgga
     5521 cgcgggatgg tacgtggcaa gttctattgg agccagacag tcccttggta cgagtggatg
     5581 tcagagcctc ggatattgca gtcgtgttcc gaagaatgtg gcctcaaaat ttggcagctt
     5641 gcagaagtca tcgcatatca gattcgtccg agtaactggc aggtatttgc atggatgagt
     5701 attgcacttc aaagtcagaa tcacgaaaag gcaatttcag atgcactcct tcagagcata
     5761 gagaaaataa agagaatgcg agatcagagt caatccgacg cacgcaccgc gctggcaaaa
     5821 cgcggtgcag cagctaagat cgcatccgac cccaaacaag cggacaaggc gttagtccgc
     5881 cagtgctggg atgactggca aaaacacccc gaccgataca agggaaagtc agcttttgcc
     5941 agagatatgc tcgacaaatt cccgaacctg aaaagtcagc gggttatctg tgacaaatgg
     6001 tgtccggctt gggaagtgga aaacatcacc ctgccagcac agtgaacacc ctgctggcag
     6061 actgcgcatc ctgctggcac ggtgctatat attaaatagc ggaaaacatt tcaaatctcc
     6121 ccgtctgctg cacttcgcgg caataagcgc cagacggcga tatgagggat tgaaatggca
     6181 attttgagaa tgacgggcgt gctggctgaa accgggctgg ccagccacgc cacggtttac
     6241 ggcgacattc atgctggtct gttcaccgtg ccagtgcaaa tcagcgaacg tgctgtgggc
     6301 tggcctgata ccgaggtgag agcaatcatt gcggcgcgga ttcgtggtga ctcagaagaa
     6361 caaatcaggg cgttggtggc caaactgcac acggcgcgta tggctggcag tgacgagcca
     6421 ttcaagacag attggtttga tcgcagcgcc caactgaaaa agtgtgcagc gcagcggcgc
     6481 aagcgcacca cgctggccac ggcataaggg gctgnnnnnn nnnnctggaa agtctcgcag
     6541 gtccgcgaat ggatgactgc gcaagcagcg gcataagggg ctggccatga ccaacaccaa
     6601 cacccagcaa aaagcccctg gagcttgcga ctccaagggc ttgcatactg acaccaacgc
     6661 atcggatttt gccagccacg ggtcggttca gcaaccccct gccagcaccg acatcgtaat
     6721 cattgaatct gattcggtca ttgccagcat gaaccgagcg catcaagctc ttgctgaagc
     6781 tgtatcggtc agccaaacca aaaccattgt tgatgtggcg cacgcggccg aaatctatgc
     6841 caagcgccag cacctgagcg agtcagccat caaccaggct acatccgtca aggtggaagc
     6901 catgcgcaag ctcggtgaga ttttgcgaga tacgccgaaa aatcaagggg ctaaggggtt
     6961 acctggtggc ggtactcgcg gtaccaaaaa ggaaccgcga gtagatgaag ctcccacact
     7021 tgatgaactt ggtttgtcaa agaaggaatc ggctgtcgct caaaagcttg caagcctgtc
     7081 agatgacgcg tttgaacagg ttcgcagcgg cagcataacc attgcgaagg ctgtagcggt
     7141 agtcgctgct gaaaaggcca aacccgagcc caagctctac ccggtcaacc cagacgttca
     7201 agacgttgag tttcgggaat ccgagccaga ggacaccggc ccggatgctg aagaactggc
     7261 agcccaggaa gcagcagagc aggccgaccg tcagaccatg caattgctcc tcgactcaga
     7321 cgacaaattt gcaaccgcag tggctgaaat caaccgcctg aaagcggagg ttttcatact
     7381 caaacagtcg cgcaatggct acatgaatga ggtcaacgcg gcaaaggcgc tggtcaataa
     7441 acgtgatcgt ttgatcagca aactaaacaa ggaaattgca gccctgaaac gaggtgctgc
     7501 atgaattcca aactttggga tgatgcgccg cactacggca gcgagacgtt cccgccacct
     7561 cgggattttc agacgacggc ccacgaggct atccgccaag cataccgtga aggtcattcg
     7621 aaaatctgcc ttatggcacc gactggtgga ggtaaaacgt atctcgggct tcgcattgcg
     7681 cacgaggcac tggtcaaggg ccgaaaggtt gtttttctgt gcgacagaac aaccttgatc
     7741 aaccagacca gctcggccgc agacagctac ggtctgagcg cccacggcat tatccaggcc
     7801 aatcactgga gacgcgacaa ggagatgccg ttccagattg cgtcagcgca gacgattcaa
     7861 aagcgtggct actggcctga tgcggatgtg atcgttgtcg atgaatgtca cacgcagatg
     7921 aaggtctgga ccgagttcat tcagacttgt cgcactgctg taattggttt gagtgctacc
     7981 ccgttcagcc cgggtctggg caagttgttt accaagctgg tcaacgcgac cacaatgcat
     8041 gagctgaccc aggcaggcgt gctggttccg atgcgagtgt tttcctgcaa acgtcccgac
     8101 atgaaaggcg cagccaccag tggcggggaa tggactgatc aggcagccgc caatcgcggc
     8161 atggagatca ttggcgacgt gatcagcgac tggcagaagt tcgcagaaaa ccggaaaact
     8221 attgtctttg gtgccaccat cgcacactgc caggaactgg cccgccagtt cgttgaggct
     8281 ggcgttatgg ccgcctgttt cacatccgag acgaccgcca ccgagcgtgc tgcccttttg
     8341 gtcgagtaca ccaaaccaga cgcaaccctg cgcgttctga tcagcgttga ggcccttgct
     8401 aaagggtttg acgtgccgac cgttgggtgc gtggttgatt gccggccact gcgcaaatca
     8461 ctgtcaacgg ccattcagat gtggggtcgc ggccttcggt catcaccaga caccggcaaa
     8521 gtggactgcc tgctgctgga ccacagcggc aacatccagc ggtttgccga cgacttcgaa
     8581 gaaatattcc accacggact gagcgccctc gacatgggcg aaaagctcga caagaaggtg
     8641 cgccaggacg acgacgagca cgaggccaaa ggctgcccag aatgcggata caaaccgttt
     8701 gccaaacgct gcatggcgtg cggatacgag aaacaggcac cctcactggt cgaaacgctg
     8761 cccggcgaaa tgcgcgaagt tgtcctcggt aaaaagaaac ttgctgatga tcatcgccac
     8821 ctatgggcgc aggtttgcac ctacgccaga tcacacagca aaccggaaaa gcagcgcgga
     8881 cgggcttgga atttgttcag aaacattacc ggtcaagcac cgtcgaacct ttggagtttt
     8941 gagacaacgc caaacgtaga aatcacccgc aacgtgcgca acaagatcac atctttgaat
     9001 gtggcgtact caaaagcgag ggcgatatga cctttattga cttcgctcgc gcccatggcg
     9061 ttgacattga cccggccaaa ctttacgcag gcgagcgcat caggcgatgc ggcaccaccg
     9121 acaaaccacg gtcggccaat ggtgcttatt tttgggatgg ccagcgaggc tgggtgttca
     9181 attgggcatc agaggcccgt gtccagtggt tcaatgacca ggatgcacag ccgtggacag
     9241 atgcagagaa acaggcatgg aaggcccgcc gccaggccat gcaagcacac caggacgacc
     9301 agcaccaccg cgccgccgct cgcgccgcag agctgattcg cagcaccaag ccgggcaccc
     9361 atgactacct ggccagaaag ggttttcctg atctggaggg gatgatcgac agcaccggaa
     9421 ctctggttgt tcccatgcgc caccttgaga caaacgcgct gcttggtgct cagcttatcc
     9481 attgggacga acccgaacgc cagtgggtca agaagatgct gcccggcatg aaagccaaag
     9541 gtgcgatcct cagacttggc gataaaacag cctcagagac gtttttggtc gaaggctatg
     9601 ccactgggtt gtcggtgctt gcagccctgc gtagcgttgg attgcgtgcg tgcgtggtgg
     9661 tctgtttcag cgcgaacaac cttgagttca tcgcgccaca ggtcaaaggc cgggttttcg
     9721 tttttgccga taacgacaaa tctggtgtcg gtgaaaaggc agccatggcc accggcaaac
     9781 cgtattgcat gagtccggtc gaaggcgagg atgccaacga tatgcacgac cgcgccggtc
     9841 tggtgccagt ggtgcaactg ttgatgaatg tcaggctggg aggtgcacat ggcgaacggt
     9901 cgtaatccaa agcgcagagg acgcgatacc agccgcgatt caggcggttt tatggcaata
     9961 ccttggtcgg tgcttgattg cccagcctac gcacgtttga gccatccggc acggtccctg
    10021 ctgctggaaa ttgcacgcca gtttgtacgc gacaacaacg gcaggctgct gacatcactt
    10081 cgccaccttg agagccgagg ctggaaaagc tgggacgtga ttactcgcgc caagcgtgaa
    10141 ctgctggagt cggggtttat ccatgaaacg gttatgggac atcgacccaa caaggccagt
    10201 tggtatgcac tcacatggcg tgcgctggac aagataccgg gctttgatcc gggcgcagaa
    10261 gcatcttttg aacgtggcgc atacatgaaa aaaattgatg tccttacacc gtctcacggt
    10321 gcagggatgc ccgttatagc accgccagac ggtgcaggcg atccgcccac tgcaccgcca
    10381 gacggtgcta tcagagccgt tttacccctc ccgcctgcac cgtctcacgg tgaccatcta
    10441 gagaagccat ctactgtggt ttgtctgtct gctggtattc cggatgtccg gctgcctgca
    10501 tccctgaccc tgattggccg ggttctgcac cgtgcccgac cggcgaaccg gcctgctggg
    10561 tatgccgtgg cccggtcccg ttttcctttt ggagtgaacc atgattagac gattcattga
    10621 ccccgacctg ctgccattga ccagcgatca aaaaaacatc ctgcttcact tggtcgcaat
    10681 gcgctactca gcacgcaagt tcattgcaag ccaaaacatc aacaggcagg ccattgccat
    10741 tctggaatca gcccaccgtg aggccattga atgtggcgac accgagggag aagcactggc
    10801 aaaaatccgg ctggaccggg ccaaagaaat cgggtttgaa ctgagattca aaatcatcga
    10861 agttggcaaa gacttcattg cggccagtgg acattacgac cagctaccgc gtgaagtttg
    10921 gctacgggcc ttgagcgtca acgaatccga gtggggcagt ccggatatgc gcaagtacgg
    10981 cgacaccatc cgcaacgtgg tcaatgtgct ggacttggaa aacagcgcta ccagggacag
    11041 cgcaatcatc aacaagcctc tgaaatggtg ctgcaccatg gggctgatgc acacatcaca
    11101 gaccaatccc gctcttggcc gggtaatgca cgatgcctgc aatgaaatgt ttggtggtgc
    11161 gtttggtgag tacgtgccgc cgaccatgct ggaacaaatg ggggtgcggc catgatttag
    11221 aaaacaccga caaacccgtt accccgaaaa cccacttatc aaggaatcaa catgctaccc
    11281 aatactttga aaataggccc attcggcgca aagcccaaat caccggacga aatcgcagca
    11341 ctcaacttgg acaatgccgc ccgaaacctg ccagacgaat gttttgcaaa cgaccccgcc
    11401 gttatcgttg ccgccgccga tgttgttgcc tggcgcaatc ggctagaccg tcttgcctca
    11461 tacccgtcca aacgcaatgc gattctgatc ttgatcagtg acattgaggc cgagcttgct
    11521 caagccaaag aagcatgccg acttgcctgt cttgattcct tcctggccga tgatcacgac
    11581 ttcattggtg ctcttgccgt acaggagcgt gtcgcactgc tggagaacag actacaggcc
    11641 gctcggacag ccaagattga tattgaacgc agctattcag aaatgaccca attgcacgcg
    11701 cttgctgaca aggctgaggc aaatcttcat aacacgcgat tcgcattgaa gcgtcaatcg
    11761 ctatctgata acgcacccaa ggaaacacgt catggcaact aaaaaactac ccagaaccgc
    11821 ctggaaaccg ggcgagtcgg ggaatccatc aggcagaccg gcgggcagtg gccgggttca
    11881 caaatggcgt gccggacttg atgccgatgt gcccgagata ttgaccgccc tggtgaaaca
    11941 ggctaaggca ggcgatgtga tggccgctcg gctgattctg gaaagggcgc tgcccgcatt
    12001 gaagccgagc gagatggcac agactatcga cctgaagggc gacacactgg cggccaaggg
    12061 cgaaagcgtg cttgttgctg tggctgatgg ttcactggca gtgagtcaag gggcacaact
    12121 gctggcggct gtcggcacat tggggcgatt ggttgaaatt gacgaactgg ccgcccgcat
    12181 cagcaaactg gaggaaaagc aaaatggcaa atcttgaatc acgaatcgag aagctggaac
    12241 aagcaatggg gcaggcacaa gaagcgccaa acatgacgct gcaattcatt cgccctgaag
    12301 ataaattctg ctgcgcatat cggcggctca agactggcga agaagtcgtt caacgcgaca
    12361 acgaatctga cgctgatttt gtggcgcgtg cgcaagccgc tggatttgaa aggacgcacc
    12421 atgagcaatc ttgaatcacg gctgaccaag ctcgaagccg ttcagcaaac tccacggcgg
    12481 caatacaccg ataccgagcg ggcagtgcgc tatgcctacc tgctggcgca aggcgggccg
    12541 cttgccgata gattgcgttc gctggttgag aaagtaccgg aggatgacca tgcaaaacct
    12601 tgaacaccgt gtcgcaaagc tggaagccga tttactgaaa gtgcggttcg atgaactgtc
    12661 cgatgacgaa ctgagggact atgcctattc gctgccgttc cagtcgccca ggcagtacaa
    12721 ggcattgatt gcgctgattt tgaggcatcc aagcacgata ccaattgttc acgatgaccc
    12781 ggcctatctt gagcgatagg gatggtgaga tggaataaac tgcaactgcg ggaggaatca
    12841 cggtgcaagg gaattgtgcc cggagccgcc accgaagagg cccccatatt tctgggggct
    12901 tcgccatttc tggcagcgcc gagatttgtc agagacgcac ctaccatcac catgaacgac
    12961 aacccattac caaaccgcca taggtatgct gctgagattg aaggctatcg gcctagcatc
    13021 accaggcgca tcctactggc gctgcacttg gtcaagccgg tgaaaggcac cagcaaggcc
    13081 aactggcatt acagcgaggt cgataccgag ggtgaattct tctgtgttct ctgccgtggc
    13141 ccggatggtc aaaacatcag caggccaatc tacgagccgc ccaagtttcc gccgaggcga
    13201 atcaagcata tttgagtgaa ttaataggta ccggccttga gaatccgcaa gccaaacata
    13261 tttttcagcg gtacggttct ttgctggcgc gggatgcttc agttagaatt tttctgcggt
    13321 cggtgttcaa gctaggcacg cgtggtaatt gcagccccga acgtcctaga agttcaccgg
    13381 cctttttcgt gtcaacgtaa gacaccaaga caatatgccg aatcttgggc gcgacggaca
    13441 agcggttatt tctgaccgaa ttgatgttac tcgctttcat gttggacaag tctggcgctg
    13501 aaaacaggcg tatgatttgg gcaggtgctt gaaaacacca agttgaagcg gtagcaactc
    13561 ccaccgaaag cggggttttt tcacgtcctg aatttgccgt tatgggtttc cccatatctg
    13621 acagttacgg gcgggatggc tagaacggta acgcctggcg cgtggcttca accatgtttt
    13681 caacatcctg tccacccatg ctgcttgaaa acggtatgtg tg
//
LOCUS       DAOSLL010000042        13554 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4639_length_13554_cov_5.192459, whole genome shotgun sequence.
ACCESSION   DAOSLL010000042 DAOSLL010000000
VERSION     DAOSLL010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 13554)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13554)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13554
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4639_length_13554_cov_5.192459"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="PLB25_03095"
     CDS             <1..394
                     /locus_tag="PLB25_03095"
                     /inference="COORDINATES: protein motif:HMM:NF016330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SWIM zinc finger domain-containing protein"
                     /protein_id="HOE40611.1"
                     /translation="LRADTLAHQVGWPQPSVHLALQVLGANGLVGFDLVHQAYFHREL
                     PFDLCLLEDLNPRLADARALLQGGAVQLVTAAPLEAVVHSADVSYRVREQDGLLRCTC
                     PWFARHQGERGPCKHVLAAQALAATMTS"
     gene            403..2973
                     /locus_tag="PLB25_03100"
     CDS             403..2973
                     /locus_tag="PLB25_03100"
                     /inference="COORDINATES: protein motif:HMM:NF040119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6493 family protein"
                     /protein_id="HOE40612.1"
                     /translation="MPQTEANPFYSTALGRCILAGDITGVMNTLDAMPTSERTAALAG
                     VQALLIDRWKLVRKPPASSASVAQTCDYDQANRLFRAVELARFMCAPDLPTCDYWMHI
                     GVQDIATFRQRYRPALSGQPLEKQLRGEYGWHYRHHIHRAVVAGLLPRPDTEEYLQSL
                     FFGDLRQESNVILKHFDADPALGPLYLALFEREGASDTSFAAVEKYCHDPALHWSTAF
                     LTLCERGIYTRTQLLDKTLGALSCDWPQFKSGWFSRFHELLAPTVDEMTAFAARYLAL
                     CQSRIAPTVTLAMDAVTALYRAGALAEAPVCDALQAVVSSAVKGRVLSALELLGQIVK
                     KDPGQGKRASGLALLALGHSAADVQKKAVACLKTWGLDAQGQEAAQTYLPLVAAVNQA
                     PLRTLLGQLPAATAAPRDTRLPSSAADSSGAVALAPASPLDPARALAPLRQLPDLIEH
                     LAYVFENPADVDAWERGAEALVRLAPLPPAAQAGFAALTKRAKRLKWDEKPLGFAMAQ
                     LMAIALGENPDLAALAPKTVALSCAADFIAWRASSLVQLARQGLGLSPLSSPTNQGGF
                     IDPQQLRARQAAFQRAGVEPPANEQELALMRVVPAIGKADVFDRKLTWSVSSSEGEYV
                     FHSLHIGVEPPIRPEMHAGLKAMLTAKHLTFGRWQTEGDGACIRFAASLMPNDLEPFF
                     AEGAHALGNNLDWWEATWQNRAYLDVLLAPTTPMTPMARLMLAVALAGKEPGQTALAI
                     DALANCLQDGRLLAHTMGDTLAAVWATPLVKGARLAKSLTAAAQAHAAMPGAVYQMLC
                     AMVAASPEAPRKDGAPLLELMLELQLAQGLALPPDTRQVLTAMRLTGKGKAARLALLA
                     "
     gene            complement(3266..3472)
                     /locus_tag="PLB25_03105"
                     /pseudo
     CDS             complement(3266..3472)
                     /locus_tag="PLB25_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3833..5530
                     /locus_tag="PLB25_03110"
     CDS             3833..5530
                     /locus_tag="PLB25_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellinolysin"
                     /protein_id="HOE40613.1"
                     /translation="MGMGINTGILSLMAQRNLSTNQSVYAASIQRLSSGLRINSARDD
                     AAGLAISERFTSQIRGQNQAIRNANDGISMLQTAEGATSSIAGSLQRIRELAVQSANA
                     TYSASDRQALQQEVNQLALEIDRISGATEFNGQKVFDQVRNKQTGYADVTKDAVLEGL
                     EGGWLEISEEMIRRSYGISASGNAISIELSTFTDGAGNTAAQVVSSVGASGPGTNIKL
                     QVDLADFATPNLPNGGTSPYYSDRIIAHEMVHAVMATAASWGEIRNNASALWFIEGAA
                     EFIHGAEERVQADTVAATLADNISTWGSASVDYSSGYVAVRYLHEKIRAAGGKGVQDM
                     LQYMQNNSGVTFDAAFANATQGAYADYAAFKADFDANKAAFVATFDFSNTDTGAIGGL
                     DVDNGPLLTATSVVEDFGNRSGQDVLSGFTETWEEVSKAGGTGNYKTFQVGANAGQTI
                     DTAIGGMNLGSLGLSTVDITSVDGSYLAMRRIDKALDYLNGTRAKLGAQMSRFETTVK
                     NLQVSSENMSASRSRVMDADFAVETAALSRAQILQNAGTAMVAQANQTPRMVLTLLR"
     gene            complement(5522..5674)
                     /locus_tag="PLB25_03115"
     CDS             complement(5522..5674)
                     /locus_tag="PLB25_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40614.1"
                     /translation="MTPVSTSSKVKVNIERYPLKQLQQARRDLDARKATGAPSRPCER
                     DAAAST"
     gene            complement(5986..6198)
                     /locus_tag="PLB25_03120"
     CDS             complement(5986..6198)
                     /locus_tag="PLB25_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HOE40615.1"
                     /translation="MFGFNEEQITNFGMTFGIGAGILYMLFIIGQLAWESKAGKFGTF
                     VMFLGLAFGFVGFVAKYLIQYFMGIK"
     gene            complement(6198..7040)
                     /locus_tag="PLB25_03125"
     CDS             complement(6198..7040)
                     /locus_tag="PLB25_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HOE40616.1"
                     /translation="MSNSQPQGQQPLPSSVLARVRTIDLAQPLQWLRRAWHDFTRCAG
                     ISAFHGVVMALTGALMLLLAHDRFWFMAGAFSGFLLVAPILATSLYALSRALERGEPI
                     GMAIVLKTWLNWQDGQFKSWGNGHWRLLQFGVLLAAAGTAWVLLSAALITVLAPVTIT
                     SPKDFIEHVVLARQGFLFELWLALGGVLVAPIFASTVITIPLLLDRQVSIRVAVATSW
                     QVVLVNPIAMALWGALIMLLTLMGFATVLLGLVLVVPLLGHASWHAYRDLLDVSALPG
                     REQR"
     gene            complement(7152..7688)
                     /locus_tag="PLB25_03130"
     CDS             complement(7152..7688)
                     /locus_tag="PLB25_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HOE40617.1"
                     /translation="MKSSLKVSLIASLLAAAGVAYAMGPMGGQCDMGAGMQGQGGMHH
                     SQMDPAKMQAKMDQRHAELKAQLKVTAAQEAAWTSYTTAMKPPPSMMAKRPDPADMAK
                     LSTPERIDKMKTLRAQHMSDMTAAMDKRGEATKVFYAALTPEQQKVFDASAMQGPGAG
                     HGRHQGPGGNQGPMQPKS"
     gene            7879..8775
                     /locus_tag="PLB25_03135"
     CDS             7879..8775
                     /locus_tag="PLB25_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HOE40618.1"
                     /translation="MFKNIIMYRIGPDWQVSPEQLEDKLQAGRFVECAASQEKSIGWT
                     EPRGQANGLLLESVANQWILKLMVETRALPASVVNRKTQERVAQIEASSGRKPGKKET
                     RDLKDDIRLELLPMAFTKQSSVLVWFDREARLLVIDAASQSRADEVVTLLVQTLPGLA
                     LSLVNTKQSPAAAMAEWLVSQEAPAGFTVDRECELKAVDESKAVVRYARHRLDMEEVR
                     QHIAEGEMPTRLALTWSERVSFMLTEGLQLKKLAFLEGVFEAGSSNKDDNFDTDVAIS
                     TGELRKLLPDLLTALGGEMPVA"
     gene            complement(8988..9179)
                     /locus_tag="PLB25_03140"
     CDS             complement(8988..9179)
                     /locus_tag="PLB25_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOE40619.1"
                     /translation="MKSNVGGIDRIVRIVLGLALIGLTLTGSIGVWGWLGVVPLVTGA
                     IGWCPPYAIFGFSTCAIKN"
     gene            complement(9613..10287)
                     /locus_tag="PLB25_03145"
     CDS             complement(9613..10287)
                     /locus_tag="PLB25_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOE40620.1"
                     /translation="MVTVPLLPLDQIIDLYPALSQVQPTLAEIGAALQPYQVPTDTVL
                     FAENTACQGFPLVIEGEIKVSRNSGDGRSLELYRVVPGELCLTSSAALFRDQVMSACG
                     VTTKPSHFLLIKPDLFNRWIDTPAFRNAVLGLFAERMADLTGLIDAVAFHKLDRRLAA
                     ALLGRGQQLNLTHQTLADELGTVREIVTRLLRRFEREGWVVLGREQIQIVNSVALRAL
                     VSGSSI"
     gene            complement(10293..10490)
                     /locus_tag="PLB25_03150"
     CDS             complement(10293..10490)
                     /locus_tag="PLB25_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOE40621.1"
                     /translation="MKHIFNVQGMTCEHCEKAVSRAIRQLDRAAVVEIDRASNRVAVD
                     SNQSPEALKQAIVEEGYSVAT"
     gene            10574..12820
                     /locus_tag="PLB25_03155"
     CDS             10574..12820
                     /locus_tag="PLB25_03155"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOE40622.1"
                     /translation="MTNLNTSTPAIRIDLGIHGMTCASCVGRAERALKKVPGVQDAVV
                     NLATESARIMVTPTEQVEARLKRAVRDAGYLPVAANAAIDARPGSGWAGFGPVALGLA
                     LSLPLMLPMLGELVGGHWMLPAWLQFALATPVQFVLGARFYKAGWHAMLSLSGNMDLL
                     VSIGTSAAWALSVWLWLTAPASATVHLYFEASAVVITLVILGKWLEARAKQQTTSAIR
                     ALHGLRPELAHLLGPDGETDVPLDEVFVDDELVVRPGERFAVDAEVLEGQTQVDESML
                     TGEPLPVAKTIGDRVTGGTLNGDGRVIIRVKAIGVDTVLAGIIRLVEDAQAGKAPIQR
                     LVDQVSAVFVPVVLLLALLTLLGWWLGGHSFELSVIQAVSVLVIACPCALGLATPAAI
                     MAGTGVAAQHGILVKDAQALELAHKARVLVFDKTGTLTVGKAQLTDFVVLPDADKNQL
                     LSLAAGLQSGSSHPLARAVVQAATAGKLRLPAVENIQTVPGKGLSGRSEENDYLIGSI
                     GWIRDQGGDFAAFQDALQRFQDQGASLAAMAHLGIDGLQVLALLTFADQPKPGASAAI
                     SALRARGIKVLMLSGDHQAAAQAMALRVGLRPELGEVISDVLPADKAAHVMALKQDGQ
                     TVVMVGDGVNDAPALAAADVGMAMANVEGGTDVAMHAAGITLMRGDLSLVAAALDISD
                     RTVAKIRQNLFWAFVYNVAGIALAALGYLNPVLAGAAMALSSVSVMSNALLLKRWHPA
                     PSQIIYTK"
     gene            complement(12901..>13554)
                     /locus_tag="PLB25_03160"
     CDS             complement(12901..>13554)
                     /locus_tag="PLB25_03160"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOE40623.1"
                     /translation="IMLEFLRLFWASTEENRFNQLSLVFLATTCLSFSPVQAADKTGQ
                     AVVQEVCANCHVSGKDGVPIIGDVAAWTKHSKNGLVKLTENAISGIGKMPAHGGQPNL
                     TDLEMSRAVAYMVSAGKAADPKKPYASPSSITGEQLVNERCVVCHDSGKDGAPRIDDF
                     AAWKPRLQKGVEGLVHSAINGHNSMPSRAGMANLSDTDMRNAVIYLVVQSATYKSKK"
BASE COUNT         2818 a         4043 c         4071 g         2622 t
ORIGIN      
        1 gttgcgagcc gacaccctgg cgcaccaggt gggctggccg caaccctcag tgcacctggc
       61 cctgcaggtg ctgggtgcga atgggctggt gggttttgat ctggtgcacc aggcttactt
      121 tcaccgcgaa ttgccgttcg acttgtgcct gctggaagac ctgaacccgc gtctggccga
      181 tgcccgcgcc ctgcttcaag gcggtgccgt gcagttggtc acagccgctc cgctggaggc
      241 cgtggtgcac agcgctgatg tgagttaccg cgtgcgtgag caggacggcc tgctgcgctg
      301 cacctgccca tggttcgcca gacaccaggg cgaacgcggg ccgtgcaaac acgtgctggc
      361 ggcccaggcc ctggcagcaa caatgacctc ttgaacctgt ttatgccgca aaccgaagcc
      421 aacccgtttt acagcacagc tctgggccgg tgcattttgg ccggtgacat caccggcgtg
      481 atgaatacgc tggacgccat gccgacgtct gagcgcactg cggccctggc cggggtgcag
      541 gcgctgctga ttgaccgctg gaagctggtg cgcaaaccgc ccgccagcag cgccagcgtt
      601 gcgcaaacct gcgactacga tcaggccaac cggctgtttc gggcggtcga gctggcccgc
      661 ttcatgtgcg cgcccgacct gcccacatgc gactactgga tgcacatcgg cgtgcaggac
      721 attgccacct tcaggcaacg ctatcggccc gcgctgtctg gccagccgct ggaaaaacag
      781 ttgcgcgggg aatacggctg gcactatcgc caccacattc accgggctgt ggtggcgggt
      841 ctgctgccgc gcccggacac cgaggagtat ctgcagagtc tgttttttgg cgacctgcgc
      901 caggagtcca atgtcattct gaaacacttc gatgctgatc cggcactggg cccgctttac
      961 ctggcgctgt ttgagcgtga gggcgcgtct gataccagct ttgcggcggt cgagaaatac
     1021 tgccacgacc cggcgttgca ctggagcacg gcctttctca cgctgtgtga gcgcggcatc
     1081 tacacccgca cgcagttgct ggacaaaacc ctgggcgcgc tgagctgcga ctggccgcag
     1141 ttcaagtccg gctggttttc gcgctttcac gagctgctgg ccccgaccgt ggacgagatg
     1201 accgcgtttg cagcacgcta cctggcgcta tgccagagcc ggattgcccc caccgtgacc
     1261 ctggcgatgg acgcggtgac tgccctgtac cgggccggtg ccctggccga agcacccgtg
     1321 tgcgatgcac tacaggcggt ggtgagctcg gcggtgaaag gccgggtact gtcggcgctg
     1381 gaactgctgg ggcaaatcgt caagaaagac cccggccagg gcaagcgtgc cagcggcctg
     1441 gcgctgctgg cgttggggca cagcgctgcc gatgtgcaaa aaaaggccgt tgcctgcctg
     1501 aagacctggg ggctggatgc ccagggccag gaagctgccc aaacttattt gccgctggtg
     1561 gcggcagtga accaggcacc cttgcggacg ctgcttggtc aactgcctgc cgcaacggca
     1621 gcgccccgcg atacccggtt gccgtccagc gccgccgatt catccggggc tgtggccctg
     1681 gccccggcat cccccctgga ccccgcccgc gccctggccc cgctgaggca actgcccgac
     1741 ctgatcgagc acctggctta cgtttttgag aaccccgccg acgtggatgc ctgggaacgc
     1801 ggggccgaag ccctggtgcg tctggctccc ttgccaccag ccgcacaggc tggttttgcc
     1861 gcgctgacaa aacgtgccaa acgcttgaag tgggatgaaa aacccttggg ttttgccatg
     1921 gcgcagctca tggccattgc cttgggtgaa aaccctgacc tggcggcgct ggcacccaaa
     1981 actgttgcgc tgtcatgcgc ggcagacttc attgcatggc gtgccagcag cctcgtgcaa
     2041 cttgcccgcc aggggctggg tttgtcgccc ttgtcctcac ccacgaacca gggcggcttt
     2101 atcgacccgc agcaactgcg ggcgcgccag gcagccttcc agcgcgccgg ggttgagccc
     2161 ccggcaaatg agcaggagct tgcgctgatg cgggtagtgc ctgccattgg taaggcagat
     2221 gtgtttgacc gcaagctaac ctggtcagtc agcagcagcg agggcgaata tgtgtttcac
     2281 agcctgcaca ttggtgtcga accaccaatc aggccggaga tgcacgccgg gctcaaggcc
     2341 atgttgacag ccaagcattt gacttttggt cggtggcaga ccgagggcga cggtgcctgc
     2401 atccggtttg cggcgagctt gatgccaaac gacctggagc cattttttgc agaaggtgcc
     2461 catgcgctgg gcaacaacct cgactggtgg gaggccacct ggcaaaaccg cgcgtacctg
     2521 gatgtgctgc tggcaccaac cacacccatg acacccatgg cccgcctgat gctggccgtg
     2581 gcactggcgg gcaaggagcc cggccagacc gcgctggcca tcgatgcatt ggcaaactgc
     2641 ctgcaggacg gccgcctgct ggcgcacaca atgggcgaca ccctggcagc ggtctgggcc
     2701 acaccgctgg taaaaggcgc gcgcctggcc aaaagtttga ccgcagcggc ccaagcccat
     2761 gcggccatgc caggcgcggt gtaccagatg ctgtgtgcca tggtggcggc gagccccgag
     2821 gcgccccgca aagatggtgc gccactgctg gagctgatgc tggagctgca actggcacaa
     2881 ggcctggcct tgccacccga cacccgccag gtgttgaccg cgatgcggct gaccggcaaa
     2941 ggcaaggcag cccggttggc cttgctggcc tagccgaatt gagcttgcag gctgcctgag
     3001 gggcgcggac ttgcctttgg cgcagtccct tccccgcggg ctccccacgg cacaagaata
     3061 tgccagcgca cacccttgaa gccgaaagac cgatattgag aacttgattc gcccgatggc
     3121 cgcttgggct gacttccacc caggcaaaaa atgtcacacc atcgcagaaa aatcaaaaac
     3181 aatagctgct tgtgcttatc ctgtatgcgc tagcagccaa tttgatgcca aagctgcggc
     3241 accccgccgc gcgccacaaa tgcgccacaa atgcgccacc agatgaccgc agcagcgcgg
     3301 caagcatggc gaaggggtgc aaaaagtcct tgaacagcag cagcacaatg ttgatgcacc
     3361 aaatgccggt caacatggcc gggccaaagc tggcagacag ctcgtccatc acttcggcat
     3421 cacgcggcgc gagttgcttc acaccgggtg gcaggggcgc tattggaaga gctagcttca
     3481 tatcgggcaa caaccgctcg aacgtattca attgatttca ctgacagtta tgcaccggtt
     3541 gcggtcagtc gaatcaaaat tgggaatatc cggtttgggt cgttatgcag agatgtgcat
     3601 aactgtcctc agccaacagt cgactctctg aactggctcc tctaagccgg gcgtcggcac
     3661 aaggacgatg atctttaggc ctttcgtatg agcgtttccc cgcatcaaaa aataaaatcc
     3721 cagtttccga gccggttgcc gaaaacaatg cagaagcggt tcaacctttg ggtggatcgt
     3781 gttagggcta tgcccgcttg cctggaaacg tcaaggaaat acggtatctg tcatgggaat
     3841 ggggatcaac accggcatct tgtcgctgat ggcgcagaga aatctgtcga ccaatcagtc
     3901 ggtttacgcc gcgtccatcc agcgcctgtc cagcggactg cgcatcaaca gcgcccgcga
     3961 cgacgcagcg ggtctggcca tttcggaacg cttcaccagt cagatacgcg gccagaacca
     4021 ggccatccgc aacgccaacg acggcatctc catgctgcag accgcggaag gtgccacctc
     4081 atccatcgcc gggagtctcc agcgcatccg tgaactggcc gtgcaatcgg ccaatgctac
     4141 ctattcggca tcggacaggc aggcgttgca gcaggaagtg aaccagttgg ccttggagat
     4201 cgaccgcatc agcggggcca ccgaattcaa cggccagaag gtctttgacc aggtccgcaa
     4261 caagcagacc ggttatgccg atgtcaccaa ggatgccgtg ctggagggcc tggaaggtgg
     4321 ctggctggag atttccgaag agatgatccg aaggtcttac ggcatctcag ccagcggcaa
     4381 cgccatcagt atcgagttgt cgaccttcac ggatggcgca ggtaacacgg ccgcacaggt
     4441 cgtctcctcg gtaggagcga gcggtccagg cacaaatatc aagctccagg tcgacctggc
     4501 cgactttgca acgcccaacc tccccaatgg tggcacttca ccttactaca gcgaccgcat
     4561 catcgcgcac gaaatggtgc atgcagtcat ggccactgca gccagttggg gcgaaatcag
     4621 aaataacgcg agtgccttgt ggtttatcga aggtgctgcc gaatttatcc acggtgcaga
     4681 agaacgcgtg caagccgaca ccgtggccgc aacgctggca gacaacatct ccacctgggg
     4741 cagcgccagc gtcgactact cgtcgggcta tgttgccgtg cgctatttgc atgaaaaaat
     4801 ccgggctgct ggcggcaagg gtgtgcagga catgctgcaa tacatgcaaa acaacagtgg
     4861 tgtgaccttt gacgcagcgt ttgcgaacgc gacccagggt gcatacgctg actatgcggc
     4921 cttcaaggca gattttgacg ccaacaaggc cgccttcgtc gccaccttcg acttcagcaa
     4981 cacggacacc ggtgccatcg gcggcctgga tgtggacaac ggccccctgc tcaccgccac
     5041 cagcgtggtt gaggacttcg gcaatcggag tgggcaggat gttctcagtg gtttcaccga
     5101 gacctgggaa gaggtgagca aggccggtgg gaccggcaat tacaaaacct tccaggtcgg
     5161 cgccaacgcg ggccagacca tcgacacggc tatcggtggc atgaatctgg gttcgctggg
     5221 cctgagcacg gtggacatca cgagcgtgga tggctcctac ctggcgatgc gacgcatcga
     5281 caaggcgctg gactacctca acggtacgcg cgccaaattg ggtgcacaaa tgtcccgctt
     5341 cgaaacgacc gtgaagaatc tgcaggtcag cagcgaaaac atgtcggcca gccgctcccg
     5401 cgtgatggat gccgacttcg cggttgaaac cgcggccctg tctcgcgcac agatcctgca
     5461 gaacgccggc accgccatgg tggcccaggc caatcagaca ccgcgcatgg tgctcacgct
     5521 gctacgttga cgcggcagcg tcccgttcac agggtctgga tggtgctccc gtggctttgc
     5581 gggcatccag atcacggcgc gcctgttgca attgcttcag cgggtagcgc tcgatattga
     5641 ccttcacctt gctgctggtg gaaaccggag tcacctggca gaccatgttg tatgcaactc
     5701 gaccaagcac aacccaagct gaattttccg caagctgaca ttggcgacgg ccaggttacg
     5761 ctgcattgca ggtccaggat tcgctccaaa gcgggtagag gcttctgatg gaaaagttgc
     5821 gaccttgcca cagtccgaag aaactgttgg ggggcgttgt gtccatcacg aaacgtgatc
     5881 attaggccct caatttgctt gatcacactc acctggccag actgaaaatc catccatttc
     5941 agtttgtaac cattttgttg aatctaggtt gaagcgagcg agtggctatt tgatgcccat
     6001 gaaatactga atcaggtatt tggcgacaaa accgacaaaa ccaaaagcca gccccaaaaa
     6061 catcacgaat gtgccgaatt tgccggcctt tgactcccag gccagttggc caatgatgaa
     6121 cagcatgtaa agaatgcctg ccccaatgcc aaaagtcatg ccaaagttgg tgatctgctc
     6181 ttcattgaaa ccaaacatca gcgctgctcc cgtcctggca aagctgacac gtccagcaga
     6241 tcgcggtagg catgccaact ggcgtggccc agcagcggca ccaccagcac cagaccaagc
     6301 aacacagtag cgaaacccat cagcgtcagc aacatgatca gagcgcccca cagcgccatg
     6361 gcgatcgggt tgaccagaac cacctgccaa ctggtggcga ccgcgacccg gatcgacacc
     6421 tgacggtcca gcagcagcgg aatggtgatc acggtagagg caaaaatcgg tgccaccagc
     6481 accccgccca gagccagcca caactcaaat aaaaacccct ggcgggccag caccacatgc
     6541 tcaatgaagt cttttggcga ggtgatcgtg acgggtgcca aaaccgtgat cagagcggcc
     6601 gacaacagca cccaggcagt acccgctgcg gccagcaaga ccccgaactg cagcaagcgc
     6661 cagtgtccgt tgccccagga tttgaattgc ccgtcctgcc agttgagcca ggtcttcagc
     6721 acaatcgcca tgccaatggg ctcaccgcgt tccagcgccc ggctcagcgc gtacaggctg
     6781 gtggccagaa tcggtgccac cagcaaaaac cccgaaaaag cccctgccat gaaccagaag
     6841 cggtcatgcg cgagcaacag catgagcgcg ccagtcaagg ccatcaccac cccgtgaaac
     6901 gcactgatgc cagcgcatcg ggtgaaatca tgccaggccc gccgcagcca ctgcagcggc
     6961 tgggccaggt caatggtgcg aaccctggcc agcacgctgg aaggcagcgg ctgttgtcct
     7021 tgtggttgag aatttgacat ggttgtggca gatgtgtgaa ctagcaatag catgcgatgt
     7081 tgccacaagc ttcgggctga caggcacaag gtgcgcctgt cagcccgatt ggtcagcagc
     7141 cttgagcggg ttcaagactt cggctgcatc ggcccctgat tgccgcccgg accctggtgg
     7201 cgaccatgac cagcacccgg tccctgcatt gccgaagcat caaacacctt ttgctgttcc
     7261 ggggtcaacg ctgcgtaaaa cactttggtg gcctcacctc gcttgtccat ggcggcagtc
     7321 atgtcactca tgtgctgcgc ccgcagggtc ttcattttgt caatgcgctc gggtgtgctc
     7381 agcttggcca tgtcagcagg gtcggggcgc ttggccatca tggatggcgg cggtttcatg
     7441 gccgtggtgt agctggtcca ggcagcttcc tgggccgccg tcaccttgag ctgggctttc
     7501 agctcggcat ggcgctggtc catcttggcc tgcattttgg cggggtccat ctggctgtgg
     7561 tgcatgccgc cctgaccctg catgccagcg cccatgtcac attgaccgcc cattggcccc
     7621 atggcatagg caacaccggc agcggccagc aggctggcga tcagcgagac tttgagactt
     7681 gatttcatga caattccttt cggttggagc tgatgaccag acagtggcat cggcgtgaaa
     7741 agaagtatcg cgcgcctgtg tatcgcggcc atgacggcaa gagaaagctt tgtaaagctg
     7801 gacgaaggtt ttaccggccc tttttggtgg gatgatcagc gccttgcaaa aaccagctca
     7861 aagcagaaag attgtcacgt gttcaagaac atcatcatgt atcgcatcgg ccccgattgg
     7921 caagttagcc cggagcaact ggaagacaag ctacaggccg gtcgctttgt agaatgtgct
     7981 gccagccagg agaaatcgat tggctggacc gagccacgcg ggcaggccaa cgggctgctg
     8041 cttgagtcgg tcgccaatca gtggattttg aagctgatgg tggaaacccg ggcgctgcca
     8101 gcgtcggtgg tgaaccgcaa gacacaggaa cgcgttgccc agattgaagc cagcagtggg
     8161 cgcaaacccg gcaaaaagga aacccgcgac ctgaaagacg atattcggct ggaactgctg
     8221 ccaatggcat tcaccaagca gtccagtgtg ctggtctggt ttgaccgtga ggcccggttg
     8281 ctggtgattg acgcagccag ccagtcccgc gccgatgaag tggtgaccct gctggtgcaa
     8341 accctgcccg gcctggcttt gagcctggtc aataccaagc agtcaccggc agcggccatg
     8401 gccgagtggc tggtcagcca ggaggcacca gcgggcttca ccgtggaccg cgaatgtgaa
     8461 ctcaaagccg tcgacgaatc aaaagcggtg gtccgttatg cccggcatcg gctggacatg
     8521 gaagaagtca ggcaacacat agccgaaggc gaaatgccaa cccggctggc gctgacctgg
     8581 agcgagcggg tgtccttcat gctgaccgaa ggcctgcaat tgaaaaagct ggcttttctt
     8641 gaaggtgtct ttgaagctgg cagcagcaac aaggacgaca actttgacac cgacgtggca
     8701 atttccaccg gtgagttgcg caaactgctg cctgacctgc tgaccgcttt gggcggcgaa
     8761 atgccggttg cctgagcctg acacatggtc ttttttggca aagcatgaag acgcctgggt
     8821 gcgtgcgaac cagttggctg cgatggttgt caaatcctca ggaaatgaat aagaaatagg
     8881 gctcaagccc tttattcatc tgcgtaacca gctattattt atgtaacaaa aaagcgcctg
     8941 ccggaaatca tccggcaggc gctttggcaa ttttgaagcc ggccttgcta attcttgatg
     9001 gcacaggtac tgaaaccaaa gatggcgtag ggcgggcacc acccaatcgc accggtgacc
     9061 agcggcacca cacccagcca gccccagaca ccgatgctgc cagtcagcgt caggccaatc
     9121 agtgccaggc ccagaacaat gcgcacaatg cggtcaatgc cgccaacatt tgatttcatg
     9181 caaactccta gaaaaaagta aaacaaccaa gataaaggga cgacaggcga attacacccg
     9241 cttggggcac atcctgtctg tgacgcaagt cacgcagcca aaagccggat tcctgagttg
     9301 accgcttgta ttcatctgtc agtcctcatg agcattttcc ttgtcagctt cgatggggtt
     9361 cgccggttgg gtgtcagcgc tccacacctg attgaaccgc tggcaacgtg actgggggcg
     9421 ttagccacgg cattcgccct gcccaggcgc gccgccagtg gcccactcgg gcgcgtccaa
     9481 ctgagaaatc caggttgaat cagctcacac cagaccttgg gtgatgattc gtgacactgt
     9541 cacactccgg cactgtggtc gtcaggcagc cgaccgtgga catcagccgg tccaaccggc
     9601 cactacgacc ggtcagatcg aactgccgct gaccagcgca cgcagcgcga cactgttgac
     9661 gatctggatt tgctcacgcc ccagcaccac ccagccttcg cgctcaaagc ggcgcagcag
     9721 acgggtgacg atttcgcgca ccgtgccaag ttcgtcggcc agtgtctgat gcgtgagatt
     9781 gagttgctga ccacgcccga gcaaggcggc ggccaatcgg cgatcaagtt tgtgaaacgc
     9841 caccgcgtcg atcaagccgg tcaaatcagc catacgttcg gcaaaaaggc ccagcacggc
     9901 attgcgaaat gccggtgtgt caatccagcg attgaacaaa tccggcttga tcaacaaaaa
     9961 atggctgggc ttggtggtaa cgccacaagc cgacatcacc tggtcgcgaa acagcgctgc
    10021 actcgacgtc aaacacagct cacctggcac cacccggtac aactccagcg aacgcccgtc
    10081 gcccgagttg cgcgagacct tgatttcacc ttcaatcacc agtggaaaac cctggcaggc
    10141 ggtgttctcg gcgaacaaca cggtgtcagt tggcacttga taaggctgca gtgcggcacc
    10201 aatctcggcc agcgtcggct ggacctggct caatgccggg tacaggtcaa tgatctggtc
    10261 aaggggcaat aacgggacag tcaccatggc tttcaggtag ctacgctata accttcttca
    10321 acaatcgcct gtttcaaagc ttcaggcgac tggtttgaat cgactgctac cctgttgctg
    10381 gcgcggtcaa tttcgacgac ggcggcgcgg tctagttgtc tgatggcgcg cgacaccgct
    10441 ttttcgcagt gttcgcaggt cattccctgg acattgaaaa tgtgcttcat gaaaatcctt
    10501 tgtaactcgg ccaatctgtc aatggccaga cgcgtattgt gaaccttcgc cagcttttgg
    10561 cacaccaaca tccatgacaa atctcaatac ctcaacaccc gccattcgca ttgacctggg
    10621 catccacggc atgacctgtg cctcatgtgt cggacgggcc gagcgggcgc tgaaaaaggt
    10681 gcccggggtg caggacgcag tcgtcaacct ggccaccgaa tcggcccgca tcatggtgac
    10741 acccacagag caggttgaag cgcggctcaa gcgcgcggtg cgcgatgccg gctacctgcc
    10801 ggtcgccgcc aacgccgcca ttgatgcccg gccaggctcg ggctgggctg gctttggccc
    10861 ggtcgcgctc ggcctggcct tgtcattgcc cttgatgttg ccgatgctgg gcgaactggt
    10921 gggcgggcac tggatgttgc cagcctggct gcaatttgcg ctggccactc cggtgcaatt
    10981 tgtactcggt gcgcgctttt acaaggccgg ttggcatgcc atgttgtccc tgagcggcaa
    11041 catggacttg ctggtgtcga ttggcaccag cgctgcctgg gccttgtcgg tctggctgtg
    11101 gctgactgcg ccggcgtcgg caacggttca cctgtatttc gaagcctctg ccgttgtcat
    11161 cacgctggtg attctgggca agtggcttga agcccgggcc aagcagcaaa ccacctcggc
    11221 gattcgcgcg ctgcacggcc tgcgccccga gctggcgcat ctgctcggcc ccgatggaga
    11281 aactgatgtg ccactcgatg aggtttttgt cgacgatgag ctggtggtcc gcccaggcga
    11341 gcgatttgcg gtggatgccg aggtgctgga agggcagacc caggtggatg agtcgatgct
    11401 gaccggcgag ccactgccgg tggccaagac aatcggcgac cgggtcacag gtggcactct
    11461 caatggcgat ggccgcgtca tcatccgggt caaggcaatc ggggttgata cggtactggc
    11521 tggcatcatc cgcctggtcg aagatgcaca ggcgggcaag gcaccgatcc agcggctggt
    11581 cgatcaggtg agtgccgtct ttgtgccggt ggtgctgctg ctggccctgc tcacgctgct
    11641 gggctggtgg cttggcggcc attccttcga gctgtcggtc attcaggcgg tatcggtgct
    11701 ggtcattgcc tgcccctgcg cgctggggct ggcgacaccc gctgccatca tggccggcac
    11761 cggggttgcc gcgcaacatg gcattttggt caaggatgcg caggcgcttg aactggccca
    11821 caaggcccgg gtgctggtgt ttgacaaaac cggcacattg acggttggca aggcacaact
    11881 gacagacttt gtggtgttgc cagacgcgga caaaaaccag cttttgagcc tggcggcggg
    11941 gcttcaaagt ggcagcagcc acccgctcgc ccgcgccgtg gtgcaggccg cgactgcggg
    12001 caaacttcgg ctacctgcag ttgagaacat tcagacggtt cccggcaagg gactctcagg
    12061 tcggagcgag gaaaatgatt acctgatcgg cagcattggc tggatccggg accaaggcgg
    12121 cgattttgcc gcctttcagg acgcgttgca gcgctttcag gatcagggtg ccagcctggc
    12181 agccatggca catctaggca ttgacggctt gcaggtgctg gccttgctga cctttgccga
    12241 tcagcccaaa cccggtgcca gcgccgccat cagcgcgctg cgcgccaggg gtatcaaggt
    12301 cttgatgctc tctggtgacc accaggcggc ggcacaggcc atggcgcttc gggtcggtct
    12361 gcggcccgag cttggcgagg tgatcagtga cgtgctgcca gccgacaagg cggcgcacgt
    12421 catggccttg aaacaagatg gtcaaaccgt ggtgatggtt ggtgatggtg tcaacgatgc
    12481 gcctgcgctg gcagcagccg atgtcggcat ggccatggca aatgtcgaag gcggtaccga
    12541 tgtcgcaatg cacgcggctg gcatcacgct gatgcgcggc gacttgtcgc tggtggctgc
    12601 agcactggac atttcagacc gcaccgtggc gaaaatcagg caaaacctgt tttgggcgtt
    12661 tgtctataac gtggctggca ttgcgctggc ggcgctgggt tacctgaacc cggtgctggc
    12721 cggcgcggcc atggccctga gttcggtgag tgtgatgagc aacgctttgc tgctcaagcg
    12781 ctggcatccg gcaccaagcc agatcattta taccaaatag tgctctagcc cttgatgcat
    12841 aagcgctgat agcttcattt agtatagcaa tcaggcgaca acacgaaagg tcaaatgggc
    12901 ttactttttg gatttgtaag tggcgctttg aaccaccagg tagatcactg cattacgcat
    12961 gtcggtgtcg ctcaggttgg ccatgccagc acgcgacggc atcgagttgt ggccgttgat
    13021 agccgaatgg accagtcctt caacgccttt ttgcagacgc ggtttccagg cagcaaaatc
    13081 atcaattcgg ggagcaccgt ctttgccact gtcgtgacag accacacaac gttcgttgac
    13141 cagttgctcg ccggtaattg aagagggcga tgcgtagggc tttttcgggt cagcggcctt
    13201 gcctgcgctc accatgtagg cgaccgcccg actcatttcc agatcggtca aattgggttg
    13261 accaccgtgg gccggcattt ttccgatgcc tgaaatggcg ttttcagtaa gcttgaccag
    13321 gccgtttttg gaatgttttg tccaggccgc tacatcgcca atgatgggca cgccgtcctt
    13381 gccactgacg tggcaattgg cgcaaacctc ctgaaccaca gcctgaccgg ttttatcagc
    13441 cgcttgcacc ggactgaaag acaggcaagt ggtggccagg aaaaccaggc tcagttggtt
    13501 gaacctattt tcctcagttg aagcccaaaa aagtcttaaa aactcaagca tgat
//
LOCUS       DAOSLL010000043        13545 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4642_length_13545_cov_4.675537, whole genome shotgun sequence.
ACCESSION   DAOSLL010000043 DAOSLL010000000
VERSION     DAOSLL010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 13545)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13545)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13545
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4642_length_13545_cov_4.675537"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..4438)
                     /locus_tag="PLB25_03165"
     CDS             complement(<1..4438)
                     /locus_tag="PLB25_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOE40624.1"
                     /translation="MKNMPLAHMVHAPAAIVIVAIDDCARPLRDLSARLVTGWLWHSA
                     RCWVALTLAALFSGLAAAATYTLPGALPAGCSSASGTSVTCPSLSLNWGDNIVVSNAN
                     LTLTVTNVLSLGGNNSINSGSVSGFAIQANSVSASNAIMLNGNLNATGTLTLSSNGNN
                     ITGNVSASGNVTIGGTVTGKVTCSGALSTQWASSITGDISAGSIASGGGSTYGGNLTA
                     TSGDITSGSGDRISGSASATSGGVTLSSSGTSVGGSVVAKNAVVLQSGVSVAGSVTST
                     NDAVTLQSSGSSVGAGVNAKKAVVLGSGSTVTGDVITDDTVTLQSSGSKVVGNIVAKK
                     NVTLANGTSVTGNVTSTIGDVELQSSSASIGSCVTVSSDQAIILGWNATVGGVCCLSG
                     STCGSACVSNNSGKAAPALCAAASAMLVAHWRFDETVSYSGAAGELKDSAGYTGGPFH
                     GAAIGNPLPAWSAVSPARPGTTGTCGYGAPGGAWSNGGAFSVSGLPVSGAAGSQTSVA
                     FWMYWDGTDSVMPIGWNVHDLWLTGGSFGFNTGNSDVFGIASSGLANGWHHVVAVFTN
                     GSVSSNQLYIDGVSQTLTQRQGTPSLASAVVSSTLRIGGWQANNDYRFFGRIDEVKLF
                     NGGISPAQVLAAYAETRTCPIRPVGDWHLDDLLWNGTTGEALDDSGSGLNGVARSGAV
                     TAAAGKICRTGAFSSATDYVEVPDAGGQLNIQAGQISVMAWIKTARGLGTIVSKTDPG
                     SPWPGYVFAVGADGGGKLAFWSKENSWKTGSTSVGNGNWHHVAAVIAGTSLKFYVDGV
                     LDQTHAITAITGTSAQNLRIGNEQNPSAPATRQFEGSIDEVLVFGAALSASEVASIVS
                     FQSAGKNWDGSPRVCPAPAALIGQYRLDESGWNGVPGEVKDSSGANRHGTAAGLPLPT
                     PSSAAPARAGNPGTCGYAILAGGSGAIPIASLPVSTTPGAKTSVGFWMYWDGTDGVMP
                     IGWNLHDLYFAGGHFGFNTANGDIYGISASGLANGWHHVVAVFVNNSVGAANKLYIDG
                     NARLLTQRLGAPHNAAAVVGSTLKISGWGANSSYRFGGRIDEVHVYDGELTVAEVQSL
                     YSATHACLSGTTPPANFNCVEAGADAASGRLFTKLAGAGFAIDAVALKADLTVQADYA
                     ASVKVELVDGAGSTACASRAALSPGVSQNLTFAAAGRKSVSLTVNKAYPDLRCRVTDS
                     NQSPSVVGCSSDNFAVRPGAVQLATTTAMATPPAAASAGTIKTGASFTLRASTTTAAS
                     DGYGGVLALDTSALTAQTTAQDATVQAGGLVGIFSPAVLTANGTPSNNANYDEVGYLY
                     LAAGAYRDSGFTAVDQGAGDCVVGSTSVVPDAGKKYGCVIGTTTGSALGRFIPDHFDV
                     TLASNGALAAACAAGGFTYTGQAMGYAAASRPALTITPRSAASGGHVTQNYQGVFQKL
                     AAAHVKLTAPVTDAAAYGKDGTTKTSLASTINAGTLSNSAGIL"
     gene            complement(4589..4777)
                     /locus_tag="PLB25_03170"
     CDS             complement(4589..4777)
                     /locus_tag="PLB25_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40625.1"
                     /translation="MSGVTAKVTATAMVIKPSTCAQAINVRFAQIAMDNDARCAPPFV
                     TCAAMRVATAAKVGFGRL"
     gene            complement(4774..5319)
                     /locus_tag="PLB25_03175"
     CDS             complement(4774..5319)
                     /locus_tag="PLB25_03175"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOE40626.1"
                     /translation="MSVQSASRAYSVEDYLEWDGNWELIGGQPVAMAPSPALGHQQAI
                     GGIFRQLDELLDDCPHCQALFEIDVEFADDSVVRPDLLVLCYTPEGDRLTRAPELVFE
                     VVSPKTASHDEVTKFDLYRTEGVAHYVLVYPDEEMAKVWRLSEGDYCKVGDFHDETHC
                     FDLSKCSLDFDFRRLWRRKGS"
     gene            complement(5704..6288)
                     /locus_tag="PLB25_03180"
     CDS             complement(5704..6288)
                     /locus_tag="PLB25_03180"
                     /inference="COORDINATES: protein motif:HMM:NF015827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HOE40627.1"
                     /translation="MSHHIAGFAPHAFNTLVGAGLLSVGMVCVVGMTPQPAQAQAQIA
                     TITAVEYCKATGGKVETYRFYDGGGQEFAKPAYVCNYPDPTGVDPSGHALVDLATLFA
                     AEPTLAALAYHAKSVWDGRFNVTPAKDYCLQLGASPTFHGSWSLKPGGDQSGMCMFED
                     GSFMEEWTLFYNQAGTPRGVDLGTVLRYPSPYIR"
     gene            complement(7024..7098)
                     /locus_tag="PLB25_03185"
     tRNA            complement(7024..7098)
                     /locus_tag="PLB25_03185"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7063..7065),aa:Arg,seq:cct)
     gene            complement(7140..11048)
                     /locus_tag="PLB25_03190"
     CDS             complement(7140..11048)
                     /locus_tag="PLB25_03190"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HOE40628.1"
                     /translation="MSTTWRLSLISLLLLLGLLALFGGLWVWSGSATSLAGALAQVTH
                     LLPASQTLEVRDVSGSLRSGGQIGWLRWQQGGLSVEARDVQIDWSVDALLDQQLQINQ
                     LAVGHLRIEDQRVSAVDELPVPLAELSLPLKVNASVSVDAVEWVGSSSQRFDKLAFNY
                     IFDSYSHRLDKGYIHILSNKYEFSGELQAHGPMALLFKADGVVNVPVSASQPPLKVTA
                     LARLDGQLAGADARLALHATLTPQSGSALAGKGGTPVTALQATLSAQLAPWQMQKIIS
                     AQGQWQALDLASLWPQTPKTLLSGAASVSPQGQGWQASIHLDNSQSGPLDQQRLPLQS
                     LRAELNYSNGQWLVQSLQAGMAGGSVVAKGQFRAGASPGEPERWNVLAEVSGINAAGL
                     YSPLSGVLIGGTLGAEQTPKGIGFDVNLQASRNKATAGAAPAATQKPATLRLQSLLAQ
                     GLWAAPMLTLTTLRLEAEDANLQGQLAYDSSSHASRGQLSLKLPGLTGTLDGHLASAD
                     GQGQLALELQDLAQTSRWLARWPQIAGLFKGYQLQGNARLSARWQGGWQNDAQALQIE
                     ARLRAPQLDWTGSASAPGEAASAARARNLQLDITGTPGSFSLSSLGLLQASEQQIDWQ
                     AQVQGGRVKSGAWQASLKQLQLSLRQGAASVPWRLQLDQASGSQPVTLNWQATELANT
                     LTVSGGAARLQGPAAGSASLNWQPLRWTQTSGAGPSALQPKPAQWQSQGRIDGVPLAW
                     LDAISHKTLADLGISGDLLLSGRWEAGQTDRLHLSVLLERSQGDLNIQADASRPQVLP
                     AGITEARLQLNLDDKKVSGNLRWDSERFGRALLAFSTELQQQGWALAPNAALGGSLQM
                     RLPPVDAWSALAPPGWRLRGTMDANVSLSGTLDAPHWAGSLQARDLALRSVVDGIDFS
                     KGTLLAKLNGQQLDIETFTLQGAGGAAGGQIDITGAVFWRTGDIGAAAKRLGLKLEAR
                     AKELRLSSRPDRRITVSGRLTAELKDTHLSLRGALKADQALLTLPDDDTPQLGSDVVV
                     RGAAASAKKPGALASSPVTGGTGTRPADSNRISPDLLVELDLGPDFQVRGRGVVTRLA
                     GKLSLAAKDVSAPSLTGTVRTVDGSYRAYGQTLEIERGLLRFTGALDNPSLAVLAIRP
                     KLSQRVGVQISGTALSPIVQLYADPDMPEADKLGWLVLGRAPSGDGAQAALLQQAALA
                     LLGRNAAGLTDGLTRALGLDEMSFSGSSSIDNADGSTSTASVTLGKRLSKDFYVAYES
                     SLNGAMGVIHIFYDLTKHLTLRAETGQQSAIDLIYTLSYD"
     gene            complement(11045..12892)
                     /locus_tag="PLB25_03195"
     CDS             complement(11045..12892)
                     /locus_tag="PLB25_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HOE40629.1"
                     /translation="MKWLAVMLLLACTAASAQGLMADAPANAAAASQSLPDAGKAAFV
                     LDIIAPDEIKQTLERHLDLQRYRTLTDLTDEELDRLLDQARLDARNLIATLGYFSPVI
                     DIERLPPGDAAASQRVSIKVTVGAPVQVSEVQLRFVGPIATDLQAAEQRQLIQESWSL
                     SAGSRFTQARWSDAKQQALRQLTTRRYPAGSVTQTLADIDPVTRQVRLELTLESGPAY
                     QLGELVVTGLGRFDADLVRRLARIPVGADYDQAALVQAQQRLTESGFFSSAFVSIDTR
                     TDPQAARVLVALREAKLQKIVVGVGASTDSGARVSVEHLHNQLPWLGWNATSKLSVDR
                     ETQYIGTSLTAPPDERGWRWNTLALLLNQTSGSFDVASQRLRAGASKETERIDRSYYL
                     QFDRADSAATDLTEFERTESITANYSFTLRSFNAMPFPTGGWAWGLELGGGTTLGSEN
                     VPFGRVLTRWQNFWQLGSSSQAPTNRHAGRLSLRGQVGAVVAKDGASLPATQLFLAGG
                     DNSVRGYSLRSIGTDLKDGQVSAGRYMVVGSLEWQHPITRNGVLTEWESVLFVDAGAV
                     ADRPADLQAKVGVGAGARWKSPVGPLQIDLAYGVDDRKWRLHMNLGFSF"
     gene            complement(12889..>13545)
                     /locus_tag="PLB25_03200"
     CDS             complement(12889..>13545)
                     /locus_tag="PLB25_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOE40630.1"
                     /translation="DDTQELGTRNIKVALRRLRKFAREGHVEELDLDDTIRCTAANAG
                     YLDIKMRPERHNNVKVLLLMDVGGTMDEHIARVEELFSAAKAEFKHLEFYYFHNCVYD
                     FVWKNNRRRYAEKFPTWDIIRKYNRDYKLIFIGDATMSPYEILQVGGSVEYSNEEAGL
                     EWLGRLTSAFPKFAWVNPEPQGVWQYRQSISIVQQLMSGRMFPMTLKGLEETMRLLSK
                     "
BASE COUNT         2535 a         4327 c         4086 g         2597 t
ORIGIN      
        1 acaggatgcc agcgctattg ctcaacgtgc ccgcgttgat ggtggatgcc aggctggtct
       61 tggtggtgcc atctttgccg tatgctgccg catcggtgac aggcgctgtc agcttcacat
      121 gagcggcagc gagtttctga aacacgccct gatagttctg ggtgacatgg ccacccgagg
      181 cagcgctcct gggggtgatg gtcaacgcgg gccgggatgc agccgcgtaa cccatcgcct
      241 gaccggtgta ggtgaagcca cctgccgcgc aagctgcggc taaggcaccg ttgctggcaa
      301 gcgtcacatc gaaatgatcg ggaatgaagc ggccaagcgc cgagcctgtc gtggtgccaa
      361 tcacacaacc gtattttttt ccagcatcgg ggacaacgct ggtactgccg acgacacagt
      421 caccagcgcc ctggtcaact gctgtgaagc cgctgtcgcg gtacgccccg gcggccagat
      481 acaaatatcc cacctcgtcg taattggcgt tgttgctggg tgtgccattg gccgtcaaga
      541 cagcggggct gaagatgcca accagcccac cggcctgaac cgtggcatcc tgcgccgtgg
      601 tttgggccgt cagggcagaa gtgtcaagcg ccagaacccc gccatagccg tcgctggccg
      661 ccgtggtggt gctggcgcgc agcgtaaagc tggcaccggt tttgatcgtt ccggcgctgg
      721 ccgctgcggg tggcgtggcc attgccgtgg ttgtggcaag ctgcacggca cctggacgta
      781 ccgcaaaatt gtcgctggag cagcccacca cgctagggct ctggttgcta tcggtcacac
      841 ggcagcgcag atccgggtaa gccttgttga cggtcagact gacacttttg cgaccggcag
      901 cggcaaacgt caggttctgc gacacgccag ggctgagtgc ggcgcgactg gcgcaggcgg
      961 tgctgccagc gccatcgacc agttcgacct tgacactggc agcgtagtca gcctggaccg
     1021 tcaggtcggc cttcaacgcc actgcgtcga tggcaaagcc agcaccggca agcttggtga
     1081 aaaggcgccc gctcgctgca tcagccccgg cttcgacaca gttgaaattg gctggcggcg
     1141 tggtgccact caggcaagcg tgggtcgcgc tgtacaggct ctgcacttcc gcgacggtca
     1201 actcgccgtc gtacacatgc acttcatcga tcctgccgcc aaaacggtag ctcgaattgg
     1261 caccccagcc actgatcttc aaggtcgatc cgaccaccgc ggcagcattg tggggtgcgc
     1321 ccaaacgctg cgtcagcagg cgcgcgttgc catcgatgta gagcttgttg gccgcaccca
     1381 cgctgttgtt gacaaaaacc gccaccacat ggtgccagcc gttggccaga cccgatgcgc
     1441 tgatgccata gatgtcgcca ttcgcggtgt tgaagccaaa atggccaccg gcaaaataca
     1501 aatcatgcaa gttccagccg atcggcatga ccccgtcggt gccatcccaa tacatccaga
     1561 aaccgacgct ggtctttgcc cctggcgtgg tgctgaccgg cagcgacgca attggaatgg
     1621 ccccactgcc gccggccaaa atggcgtaac cacaggtgcc agggttgccg gcccgggccg
     1681 gtgccgcaga cgacggtgtc ggcaagggca aacccgctgc cgtgccatgc cgatttgccc
     1741 cgctgctgtc cttgacttca ccgggcacac cgttccagcc actctcgtca agccgatact
     1801 ggccaatcag cgcggctggt gccgggcaaa cacggggtga gccgtcccaa ttcttgccag
     1861 cactctgaaa agacacgatg ctggcgacct cgctggcgct cagtgcggca ccaaagacca
     1921 gcacctcgtc aatgctgcct tcgaactgcc gggtggccgg tgccgatggg ttttgctcat
     1981 tgccgatgcg cagattttgt gccgaggtgc cggtgatggc cgtgatggcg tgggtctggt
     2041 caagcacacc atcgacataa aacttcagcg aagtacccgc aatcactgcc gcaacatggt
     2101 gccaattgcc attgccaacg ctggtgctgc cggttttcca ggaattttcc ttggaccaga
     2161 aggccagctt gccgccaccg tcggccccca cggcaaacac atagcccggc cagggcgaac
     2221 cggggtcggt tttcgagacg atggtaccca ggccacgcgc cgtcttgatc caggccatga
     2281 cgctgatctg ccccgcctga atgttgagtt gcccgccagc atcgggtacc tcgacgtagt
     2341 ctgtggctga actgaaggcg ccggtgcgac aaatcttgcc cgcagcagcc gtcaccgcac
     2401 cactgcgcgc cacgccgttc aggccagagc cgctgtcgtc aagagcctcg ccggtggtgc
     2461 cattccacag caaatcgtcc agatgccagt cacctaccgg gcgaatcggg caggtgcggg
     2521 tttcggcata ggcagccaac acctgcgccg ggctgatgcc gccgttaaaa agcttgactt
     2581 cgtcaatacg gccaaaaaag cggtagtcgt tgttggcctg ccagcctccg atgcgcaagg
     2641 tgcttgaaac cactgcgctg gcgagactgg gcgtgccctg gcgctgcgtc aaggtctggc
     2701 tgacaccatc gatgtacaac tggttgctgc tgacgctgcc attggtgaac accgccacca
     2761 catggtgcca gccgttggcc aacccgctgg atgcgatgcc aaacacgtcg ctattgccgg
     2821 tgttgaaacc gaaactgcca ccggtcaacc acaagtcatg cacgttccag ccaattggca
     2881 tgacactgtc ggtaccatcc caatacatcc agaacgccac cgatgtctgg ctaccagccg
     2941 cacctgacac cggcaaaccc gataccgaaa acgcaccgcc attggaccag gcaccgcccg
     3001 gagcgccata gccacaggtg cctgtcgttc cgggccgagc ggggctgacc gcggaccacg
     3061 caggcaaggg gttgccaatt gccgccccgt gaaacggtcc accggtgtag cccgcactgt
     3121 ccttgagttc accagccgcc ccgctgtacg acacggtctc gtcaaagcgc caatgcgcca
     3181 ccagcattgc cgaggccgct gcacacaagg ccggtgccgc cttgccgctg ttgttgctga
     3241 cgcaagcgct gccacaggtg ctgccggaca ggcagcagac accgccaacg gtggcgttcc
     3301 agcccaaaat gatggcttgg tcgctgctga ccgtcacaca actgccgatg gacgcggatg
     3361 aggattgcaa ctcgacatca ccaatcgtgc tggtgacgtt gccagtcacc gaggttccat
     3421 tcgccagcgt gacgttcttc ttggccacaa tattgccaac caccttgctg cccgaacttt
     3481 gcaaggtcac cgtatcgtcg gtgatcacat caccggtgac cgtgctgccg ctgccgagga
     3541 cgacggcttt tttggcgttg acaccggcac ccacactgct gcccgagctt tgcagcgtga
     3601 cggcatcatt ggtgctggtc acgctgccag ccaccgacac accgctttgg aggacaactg
     3661 cgtttttggc gacaacgctg ccgccaacgc tggtgccaga gctactgagc gtcacgccgc
     3721 cgctggtggc gcttgcactg ccggagatcc ggtcgcccga gccactggtg atatcgccac
     3781 tggtggcagt caaattgccg ccataggtgc tgccaccgcc actggcaatg ctgccggcac
     3841 tgatgtcccc ggtgatgctg ctggcccatt gggtggacaa cgcgccgctg caggtgacct
     3901 tgccggtaac cgtgcctccg atagtgacat tgccgctggc actcacattg ccggtgatgt
     3961 tgttgccgtt gctgctcagc gtcagggtgc cggtcgcgtt gagattgcca ttgagcatga
     4021 tggcattgct ggcgctgacg ctattggcct gaatggcaaa gccgctgaca ctgccgctgt
     4081 tgatgctgtt gttgccgccc aggctgagca cattggtcac cgtcagcgtc aggttggcat
     4141 tgctcaccac gatgttgtcg ccccagttca ggctcaggct cgggcaggtg acactggtgc
     4201 cagatgctga actgcaaccc gcaggcaaag cacccggcag cgtgtaggtg gccgctgccg
     4261 ccaagccgct gaagagagcc gctagcgtca gcgccaccca gcacctggcc gaatgccaaa
     4321 gccagcccgt gaccagccgc gcagataggt cacgcagggg tctggcgcag tcatcaattg
     4381 ctactataac aatagctgct ggcgcatgca ccatatgggc tagaggcata tttttcataa
     4441 attttccgaa agctccggcg ctaccgtcgt caacacagca acctgcaaca atggcaaccg
     4501 ccgcccctgc caccgcccgc cgattcaatg gttttcacat tgaccggcga tggtggatcc
     4561 ggccgtaaaa atgccatggt tgacgcgatc acaagcggcc aaagccgact ttggccgcag
     4621 ttgccacgcg cattgcggca caggtgacaa atggcggggc gcagcgcgcg tcgttgtcca
     4681 tggcgatctg ggcaaaccgc acattgatgg cctgcgcgca agtcgagggt ttgatgacca
     4741 tggcggtggc ggtgaccttg gccgtcacgc cactcaagat cccttgcgcc tccataggcg
     4801 gcggaagtca aagtcgaggc tgcacttgct caagtcaaaa cagtgcgtct cgtcatggaa
     4861 atctcccacc ttgcagtagt caccctcaga cagccgccag accttggcca tttcttcatc
     4921 cggatagacc agcacatagt gggccacacc ctcggtgcgg taaagatcaa acttggtgac
     4981 ttcgtcatgg ctggcggttt tgggcgaaac cacttcaaaa accagctcgg gtgcacgggt
     5041 caaccggtcg ccttcgggcg tgtaacaaag caccagcaga tcgggccgca ccacgctgtc
     5101 atcggcaaat tcaacatcaa tctcgaacag cgcctggcag tgcgggcagt cgtcgagcaa
     5161 ctcgtcgagc tgtctgaata tgcctccaat ggcctgctga tgcccaagcg ctggcgatgg
     5221 tgccatggcg actggctgcc cgccaatcag ctcccaattg ccatcccatt ccaggtaatc
     5281 ctccacgcta taggctctcg atgctgactg gacggacatg cttctgggac tcctgctatc
     5341 gggtttaggt caaattccca ttctacgcaa aggccttcag tacagacttt atgcggctgg
     5401 aaagctgcaa caaacgccaa atcatgctga gcctttgaca cacttcgcca aatgctgtca
     5461 acggggtaga agcttgcagc atctctgctg cgctgacctg acatcgacga ctgaaccatt
     5521 gacctgaacc atcgacactt tcggtcgcac cggtggatgt ttcaattttg gagtatcagg
     5581 ttgacaagat ggctgttttg ctgcccgcag atgctcttgc tatctggcca gtcaagccct
     5641 tcactgaatc gcccaggact cttgcgcaag tggcaactca ttgagttcca cccgccgcgc
     5701 agatcagcga atataggggc tggggtatct caacaccgtg cccagatcca caccacgcgg
     5761 tgtgcccgcc tggttgtaga acaacgtcca ctcttccatg aacgagccat cttcaaacat
     5821 gcacataccc gactggtcgc cgccaggctt caggctccaa ctgccatgga acgtggggga
     5881 cgcaccaagc tgcaggcaat agtccttggc cggcgtaacg ttgaatctgc cgtcccaaac
     5941 cgacttggca tggtaggcca gggcagccag cgtgggctcg gccgcaaaca gcgtggcgag
     6001 atcaaccagg gcgtggccgc tggggtcgac accggtgggg tcggggtaat tgcagacata
     6061 ggcgggcttg gcaaactcct ggccaccccc atcgtagaag cgataggttt ccaccttgcc
     6121 gccggtcgcc ttgcaatact ccaccgccgt tatggtggcg atctgcgctt gcgcttgcgc
     6181 gggctgcggg gtcatgccca caacgcagac catccccaca gacaacaggc cagcgcccac
     6241 gagggtattg aacgcatgag gggcgaaccc cgcaatgtga tgagacataa gatttccaaa
     6301 ggtgagtctg aatgggaaat tcaaccgcga gtaggcaaac atgttacagg ctttcggcca
     6361 caaagtgcct ttgaccagtg cagacggcgc aagcactgca gccccatggc atgagccccg
     6421 ggcgaaaggc ggacttcagc ttgggcgcag gcccaccacg cacgcaaccc tgccccacgg
     6481 tgcatgtgca tcgccgattc ctgcttcacc tgttgagaca ccggtccacg tcgaagtccg
     6541 gtgcgggatc cgtttcgttc agatgcctga ttcccgaacg tacggttttg ccagcccccg
     6601 tttgccagca agcaaggctt gtgtatcgct gcaatggaaa ccgtgaatcg tgcgcaggta
     6661 acaacgcgag cgttgtcact attcaaggcg ccagacaccc attgccggtg cgccggtgcc
     6721 ctggcgtgct gcggccaacg tcagcacctc gaccgaccgg ccatgagctg gtggcagcct
     6781 gcaccaggtg cgaaacctcg aaagaaagcc gcctgagtgc cagacattgc tttttttggg
     6841 tgattgagcg ccttcacacg ggcgattcat gcccctgatt tcaaaacatt tgcccaaaaa
     6901 tgggctccct gttgatgctg aaatggggca catttgggcc aacgctagca agcaacaaaa
     6961 gacggttttc ataggggaaa cgaaaaaaaa ccggactgca aaatacatgt ccggtctttg
     7021 atttggtccc gctgacagga atcgaacctg tatctagcac ttaggaggca ctcgttctat
     7081 ccattgaact acagcgagaa gctgggcgca ttgtaaatgc ggccccggtc agggcagcat
     7141 cagtcgtagc tcagcgtgta aatcaggtcg atggcacttt gctggccggt ttcggcgcgc
     7201 aaggtcaggt gtttggtcag gtcataaaaa atgtggatca cacccatggc accgttgagg
     7261 ctgctttcat acgcgacata aaagtctttt gacaagcgct tgcccagggt gaccgacgct
     7321 gtgctggtgc tgccgtctgc gttgtcaatg ctgctgctgc cgctgaaact catttcgtca
     7381 agccctaaag ctcgcgtcag tccgtcggtc agcccggcgg cattgcggcc cagcaaggcc
     7441 agcgcggcct gctgcagcag ggccgcctgg gcaccgtcgc cactgggtgc gcggcccagc
     7501 accagccatc ccagtttgtc ggcttcgggc atatccgggt cggcatacaa ctgcacgatt
     7561 ggcgacagtg cggtgccact gatttgcaca ccaacgcgct ggctgagttt cgggcgaatc
     7621 gccagcacgg caagcgacgg gttgtccagc gctccggtaa agcgcagcag gccgcgctcg
     7681 atttccagcg tctgcccata ggcacgatag ctgccatcaa ccgtgcgcac ggtaccggtc
     7741 aggctaggcg cagaaacgtc tttggccgcc agcgacaact tgccggccag ccgggtcacc
     7801 acgccccgcc cgcgcacctg aaagtctggc cccaggtcca gttcaaccag caggtccggg
     7861 ctgatccggt tgctgtctgc cgggcgcgta ccggtgccgc ctgtcaccgg tgaggatgca
     7921 agggcgcccg gctttttggc gctggccgct gcgccgcgca ccaccacgtc gctgccaagc
     7981 tgcggtgtgt catcgtcggg cagcgtcagc agtgcctgat ctgccttcag cgcaccgcgc
     8041 aggctcaagt gcgtgtcctt gagttcggca gtcaggcggc cagatacggt gatgcggcgg
     8101 tcgggccggg aactcaggcg cagttccttg gcccgggctt cgagcttcag gcccagtcgc
     8161 ttggccgccg cgccgatgtc gccagtgcgc cagaacactg cgccagtgat gtcaatctgg
     8221 ccaccggcgg caccgcccgc gccctgcagg gtgaaggttt cgatgtcaag ctgctgcccg
     8281 ttgagtttgg ccagcagcgt ccccttgctg aaatcaatgc catcgacgac tgagcgcaat
     8341 gccagatccc gggcctgcaa actgccagcc cagtgtggcg cgtccagcgt gcccgacagg
     8401 ctcacgttgg catccatggt gccgcgcagt cgccagccag gtggtgccag cgccgaccag
     8461 gcatcgactg gcggcaagcg catctgcagg ctgccgccca gcgctgcgtt gggggccagc
     8521 gcccagccct gctgctgcaa ttcggtgctg aaggccagca gcgcgcggcc aaaccgctcg
     8581 ctgtcccagc gcaggttgcc tgacactttt ttgtcatcca ggttcagttg caggcgcgcc
     8641 tccgtgatac cggcgggcag cacctgcggg cggctggcat cggcctggat gttcaggtca
     8701 ccctgactgc gctccagcag caccgacaga tgcaggcggt cggtttggcc ggcttcccaa
     8761 cggcccgaca gcagcaggtc gccgctgatg cccagatcgg ccagcgtttt gtggctgatc
     8821 gcatccagcc aggccagcgg cacgccgtcg atgcggccct ggctttgcca ctgcgcgggc
     8881 tttggctgca gcgccgatgg ccccgcgccg ctggtttgcg tccagcgcaa gggctgccag
     8941 ttcaagctgg cgctgcccgc agccggaccc tgcagacgtg ccgcgccgcc agagaccgtc
     9001 agcgtgttag ccagctcggt ggcttgccag ttcagtgtga ccggctggct gccagatgcc
     9061 tggtcaagtt gcaaccgcca aggcacgctg gctgcgccct ggcgcaatga caactgcagt
     9121 tgcttgaggc tggcctgcca ggcgccgctc ttgacgcggc cgccttgcac ctgtgcttgc
     9181 cagtcgatct gctgctcgct ggcttgcagc aaacccaggc tgctcaggct gaagctgccc
     9241 ggtgtgccgg tgatgtcaag ctgcaggttg cgggcgcgcg cggcgctggc agcttcaccc
     9301 ggcgcgctgg cgctaccagt ccagtcgagc tgtggtgcgc gcagtctggc ctcgatctgc
     9361 aaggcctgcg catcgttttg ccagccaccc tgccaccggg ccgagagtct ggcattgccc
     9421 tgcagttggt agcctttgaa cagaccggca atttgcggcc agcgtgccag ccagcggctg
     9481 gtctgtgcca ggtcttgcaa ctccagcgcc agttggccct gaccgtctgc gctggccagg
     9541 tggccgtcca gcgtgccggt cagccctggc agcttcaggc tgagttgccc gcggctggcg
     9601 tggctgctgc tgtcataggc aagctggccc tgcaagttcg cgtcttcagc ctcaaggcgc
     9661 agggtggtga gggtcagcat tggtgccgcc cacaggcctt gggccagcag gctttgcagg
     9721 cgcagggtgg ctggtttttg ggtcgctgct ggtgctgcgc cggcagttgc cttgttgcgg
     9781 ctcgcctgca ggttgacatc aaagccaatg cctttgggcg tttgctccgc gcccagcgtg
     9841 ccgccaatca gtaccccgga caagggcgaa tagagcccgg ctgcgttgat gccacttacc
     9901 tcggccagga cgttccagcg ctcaggctcg cctgggcttg cgccagcgcg aaattgcccc
     9961 ttggccacca cgctgccccc ggccatgcct gcctgcagtg actgcaccag ccattgcccg
    10021 ttgctatagt tcagttcagc ccgcaggctt tgcagcggca ggcgttgctg atcaagcggg
    10081 ccgctttggc tgttgtcgag gtggatgctg gcctgccagc cctggccctg cgggctaacc
    10141 gaggcggcac cactgagcag cgttttgggg gtctgcggcc acaggctggc caggtcaagc
    10201 gcctgccact ggccctgggc cgaaatgatt ttttgcattt gccagggtgc cagttgggct
    10261 gacagcgtgg cctgcagggc ggttaccggg gtgccgcctt tgccagccaa cgcgctgccg
    10321 ctctggggtg tcagcgtggc gtgcaacgcc agcctggcat ccgcaccggc cagttgcccg
    10381 tccagccggg ccagcgcggt gaccttgagc ggcggctggc tggccgaaac cggcacgttc
    10441 acaaccccgt cagccttgaa cagcaaggcc attggcccgt gtgcctgcaa ctcccccgaa
    10501 aactcatatt tattggacaa aatatggatg tagcccttgt ccagtctgtg cgagtagcta
    10561 tcaaaaatat agttaaatgc gagcttgtca aaacgctggc tactgctgcc aacccattcc
    10621 acagcatcga cactgacgct ggcgttgacc ttcagtggca ggctcagttc agccagcggc
    10681 actggcagct cgtcaaccgc gctgacgcgc tggtcttcaa tgcgcagatg accgactgcc
    10741 agttggttga tctgcaactg ctggtccagc agggcgtcca ccgaccagtc gatttgcacg
    10801 tcgcgtgctt ccaccgacaa accgccttgc tgccagcgca gccagccaat ctgcccaccg
    10861 ctgcgcagcg agccgctgac atcacgcact tccagcgttt ggctggctgg cagcaaatgc
    10921 gtcacttgtg ccagcgcacc ggccagtgaa gtggcgctgc cgctccacac ccacaagcca
    10981 ccgaacaacg ccagcaagcc cagcagcagc agcaagctga tcaggctgag tcgccaggtg
    11041 gtgctcaaaa gctgaacccc aggttcatgt gcagccgcca tttccgatca tcgacaccgt
    11101 aagccaggtc aatctgcagc gggccgaccg ggcttttcca gcgcgcaccg gcacccacgc
    11161 cgaccttggc ctgcaggtcg gccggacggt ctgccaccgc acctgcatca acaaacagca
    11221 ccgattccca ctcggtcaac acgccgttgc gggtgatcgg gtgctgccat tccagactgc
    11281 ctaccaccat gtagcgaccg gcgctgactt gcccgtcctt gaggtcagtg ccgatgctgc
    11341 gcagactgta accccgcaca ctgttgtcgc cacccgccaa aaagagctgg gtggcgggca
    11401 agctcgcccc gtccttggcg acgaccgcac caacctgccc gcgcagcgac aagcgcccgg
    11461 catgccggtt ggtcggcgct tgactgctac tgcccaactg ccagaaattt tgccagcggg
    11521 tcaacacccg gccaaaaggc acgttttcgc tgcccagcgt ggtgccacca cccaattcca
    11581 gcccccaggc ccagccgccg gtcggaaatg gcatggcgtt gaaactgcgc agggtgaagc
    11641 tgtagttggc cgtgatggac tcggttcgct cgaattcggt caggtcggtg gcggccgagt
    11701 cggcccggtc aaactgcagg taatagctgc ggtcaatgcg ctcggtttcc ttgctggcac
    11761 cggcgcgcaa gcgctggctg gcgacgtcaa agctgccact ggtctggttc agcagcagtg
    11821 ccagcgtgtt ccagcgccag ccgcgctcat caggtggcgc ggtcagactg gtgccgatgt
    11881 attgggtttc gcggtcaacc gacagtttgc tggtggcgtt ccagcccagc cagggcaact
    11941 ggttgtgcaa atgctcgacg ctcacgcggg caccgctgtc ggtgctggca cccacaccga
    12001 cgacgatctt ttgcaatttt gcctcgcgca gggccaccaa cacccgagcg gcctgcggat
    12061 cggtgcgggt gtcaatcgag acaaaggcgc tgctgaaaaa accgctttca gtcagccgct
    12121 gctgtgcctg caccagcgcc gcctgatcgt agtcggcccc caccggaatg cgtgccaggc
    12181 ggcgcaccag gtccgcatcg aagcgaccga ggccagtgac gaccagctca cccaattggt
    12241 aagccggccc ggactccagc gtgagttcga ggcgcacctg ccgtgtcacc gggtcaatgt
    12301 cggccagtgt ctgggtgacg ctgccagcgg gatagcgccg ggtggtgagc tgacgcagcg
    12361 cctgctgttt ggcatcgctc cagcgcgcct gggtgaatcg gctaccagcg ctgagcgacc
    12421 agctttcctg aatcaattgg cgctgctctg ccgcctgcaa gtcggtggcg atgggaccga
    12481 caaatcgcaa ctgcacctca ctgacctgca ccggcgcacc gaccgtgacc ttgatgctga
    12541 ctcgctggga ggccgcagca tcaccagggg gcaaacgctc aatgtcgatc accggcgaaa
    12601 aataccccag cgtggcgatc agattcctgg catcaagccg ggcctggtcg agcagacggt
    12661 cgagttcttc gtcggtcaga tcggtcaggg ttcggtagcg ctgcaggtcc aggtggcgct
    12721 caagtgtctg tttgatctcg tcgggtgcaa tgatgtcgag cacaaatgcg gccttgccag
    12781 cgtcgggcag gctttggctg gctgcggctg catttgccgg ggcatcggcc atcaagccct
    12841 gcgctgatgc ggcggtacag gccagcagca acatgaccgc cagccacttc atttgctcag
    12901 cagacgcatg gtttcttcca gtcctttgag ggtcatgggg aacatgcgcc cgctcatgag
    12961 ctgctgcacg atgctgatgc tttgacggta ttgccagaca ccctgaggct cggggttaac
    13021 ccaggcaaat ttgggaaaag cgctggtcag ccgccccagc cactccagcc cggcctcttc
    13081 gttgctgtat tccacactgc cgccgacctg caggatttca tacgggctca tggtcgcatc
    13141 gccaatgaaa atcagcttgt agtcgcggtt gtatttgcgg atgatgtccc aggtgggaaa
    13201 cttctcggca tagcggcggc ggttgttttt ccagacgaag tcgtacacgc agttgtgaaa
    13261 gtaataaaac tccaggtgct tgaactcggc cttggcagca gaaaacagct cttcgacgcg
    13321 ggcaatgtgc tcgtccatgg tgccgcccac atccatcagc agcagcacct tgacgttgtt
    13381 gtgccgctca gggcgcattt tgatgtccag atagccggca ttggcggcag tgcagcggat
    13441 ggtgtcgtca aggtcgagtt cttccacatg cccttcgcgg gcaaacttgc gcaggcggcg
    13501 cagcgccacc ttgatgttgc gtgtgcccag ctcctgcgtg tcgtc
//
LOCUS       DAOSLL010000044        13544 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4644_length_13544_cov_5.270962, whole genome shotgun sequence.
ACCESSION   DAOSLL010000044 DAOSLL010000000
VERSION     DAOSLL010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 13544)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13544)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13544
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4644_length_13544_cov_5.270962"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLB25_03205"
     CDS             <1..470
                     /locus_tag="PLB25_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019431191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOE40631.1"
                     /translation="DFIVSKTDLKGRITYCNRVFIEFSGYNSSELIGQQHNIVRHPDM
                     PRGVFKFLWDTLEQNKECFAFVKNMCKDGSFYWVFANVTPDLDAKGLVVGYFSVRRKA
                     SAGAIALMTDLYKAMLEEERKVGPKDACAASLALLANTLQQKGVSYEQLVLSL"
     gene            445..2457
                     /locus_tag="PLB25_03210"
     CDS             445..2457
                     /locus_tag="PLB25_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE40632.1"
                     /translation="MSNSSSRSESSAYVWTMALLLVAGAALQSWHTGWSPFVLVWLLP
                     GLLCLLFWYLSLQRQHRLMMDIQSISQQVAHGQLGRRLDKIPASGLFHQLCWDFNDML
                     DQLEACFREQATALQYASSGQYFRLAQATGLRGTFGKSLEQTNQSLLAMAQTAAAEQQ
                     AAAEKLAAQEKERVVANANRRIRLALDNVSLPVRIADDNGTVIYINNALRDTLHRNAE
                     GFRQQIPGFDPAKVVGGSIGMFYADAEAALTRLRDLTAATQTRLKLGGRMYDLTTTPV
                     LSQEGERLGTVGQWQDVTEQLAAEEEIDQMVQAAAQGNLSQRLSLHGKSGFFANLSNG
                     MNQLVQTTEQGLSDVAQVLSAVADGDLTQRITRDYAGLFGKVRDNVNASAENLTRVMS
                     EVRAAANALSDAANQVSATAQSLSQASSEQAASVEQTTASIDVMSVSITQNSDNAKVT
                     DGMATKTTKEAVEGGVAVSQTVVAMKQIASKIGIVDDIAYQTNLLALNAAIEAARAGE
                     HGKGFAVVAAEVRKLAERSQEAAKEIGELAGSSVITAERAGRLLEQIVPSIQKTSELV
                     QEIAASSSEQSSTVTEIGGAMGQLSKATQQNASASEELAATSEELSGQAEQLQQSIAF
                     FNIGDDAPAVPDRHARAPNMKMPAQYPMPRLRSSPKASAQGNFKPY"
     gene            2484..3449
                     /locus_tag="PLB25_03215"
     CDS             2484..3449
                     /locus_tag="PLB25_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOE40633.1"
                     /translation="MSQDKDIEERTRLAGTNKFELLLFRLGESPSTGERELYGINVFK
                     VREVLVMPKITALVNSHEHLMGVANIRGQIIPVINLPAVVGCQPKKGLSILMVTEFAR
                     TIQGFAVEDVREIVRLDWDQVLPAESSHAGALVTGIARLDGARADTRLAQVLDVEEVL
                     RDVMPVTKEEINREVVGPELLLPEGTSILVADDSHVARAVIELGLKAMGAPFVMTHTG
                     RQAWDYLQAAADKAKADGTRVKDNISVVLTDLEMPEMDGFTLTRLIKQDARFKGIPVV
                     IHSSLTGTTNENHVKGVGADGYVGKFSPRELANTLRQVLAGQVSH"
     gene            3469..5544
                     /locus_tag="PLB25_03220"
     CDS             3469..5544
                     /locus_tag="PLB25_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010396792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE40634.1"
                     /translation="MKSTQYSWLARMFHGAEINRRLADLEQELKVRTDIMNLTSIVSE
                     SDKKGDILSINDKFVEVSKYSKDELLGHPHSTTRHPDMPKETFRQLWATIGRGAMFRG
                     VIKNRAKDGAPYYVDAVIAPFMGDNGKPMKYLGVRYDVTQAETERQNARGILGAVDAS
                     FGMVEFDLGGHVLQANVNFLQIMGYSSAEVSGKNHRLFVEASLAASPAYQQLWADLKA
                     GKPQKGTFKRVGKGGKEVWLQAVYSPVMDEMGRVLKVVKIATDVSSEFQANAMLQTAV
                     HDSLAVIAAAKDGDLSQRVDLGGKAGPIASLCTGVNTLLDTTVIAFADIGRVLGAMSA
                     GDLSQRVTREYAGTFNQLKQDANVTSEKLSNIIDDMARVFEGLASGDLTQRITRDTEG
                     AFERVKQDANLSFEKLAAIIEEVRAAADALTGAANQVSATAQSLSQAASEQAASVEQT
                     TASIDVMSDSITHNSDNARVTDGMATKTTKEAVDGGSAVSQTVTAMKQIAAKICIVDD
                     IAYQTNLLALNAAIEAARAGEHGKGFAVVAAEVRKLAERSQEAAKEIGELAGNSVTTA
                     ERAGRLLDAIVPSIQKTSELVQEIAAASAEQSESVSMIGEAMGQLSKATQQNASASEE
                     LAATSEELSGQAEQLQQAVGFFNLGGAAPQLRSRHELPAPERRGAPERKPAPRLELPG
                     RSGGNFKPY"
     gene            6939..7049
                     /locus_tag="PLB25_03225"
                     /pseudo
     CDS             6939..7049
                     /locus_tag="PLB25_03225"
                     /inference="COORDINATES: protein motif:HMM:NF037854.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7467..8558
                     /locus_tag="PLB25_03230"
                     /pseudo
     CDS             7467..8558
                     /locus_tag="PLB25_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            8570..9388
                     /locus_tag="PLB25_03235"
     CDS             8570..9388
                     /locus_tag="PLB25_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HOE40635.1"
                     /translation="MSAITFSDREFKRISEIMYEAAGLSFNDSKKPLIQSRLAPRIQR
                     MGLASFGDYIALLEDESQAAEFQMAVDMLTTNETYFFREPRHYDLLEQELTARKSRAP
                     LAIWSAAASFGDEAYSTAMLLSDMQVQGRVGPDWSILATDISHRVLLSAKEAVYPADR
                     LRAVSPERLKRYCLRGDGESEGQVLIQDKIRQRVQFGRLNLCQPIDGVGPFDIIFLRN
                     VLIYFDIPTKIEVVDRVLTTLRPGGLFFLGTAEGRVACKTPLLTVQPGAFRKPA"
     gene            9404..9862
                     /locus_tag="PLB25_03240"
     CDS             9404..9862
                     /locus_tag="PLB25_03240"
                     /inference="COORDINATES: protein motif:HMM:NF015908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOE40636.1"
                     /translation="MATFELMPGDVALGYAGDQLKTLLGSCVSVILTDPRRTVGVMCH
                     IVHVGHPNTANRNNTAYGSVAMADMTRRLYAIGFAPRSCQAYVVGGGNMFPKLFTHHH
                     VGASNIDWVLGYLEHHKIKVLRQDLGGTGYRKLVWTVGGSDPTVETASSN"
     gene            9877..10956
                     /locus_tag="PLB25_03245"
     CDS             9877..10956
                     /locus_tag="PLB25_03245"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOE40637.1"
                     /translation="MVVKVFVVDDSAVVRQALAHMLAGNSDVELIGSAPNPLLAMAAI
                     RKNPPDVLLLDIEMPGMDGLTFLRQIMAGTPIPTVICSTLSTEGSRVALDALSAGAVA
                     VLAKPKLGLKQHLDDSRLEMIQTLKTAARSRPRARGAMPSIAAKAITTGVAAQIGRMP
                     APSGVHALAMNKPVVIGSSTGGTQALELVLTSLPADAPGIAITQHMPEKFTAMYAQRL
                     DGLCAMNVREAKDGDRLERGVALIAPGGRHMQLRKTNGQYFVKVLDGPPVNRHKPSVD
                     VLFKSAAECAGRDALGIIMTGMGDDGARGMKVMHDGGARTIAQNEETCVVFGMPKEAI
                     KLQAVDDILPLEGMARAILQFDSRA"
     gene            complement(11032..12030)
                     /gene="gap"
                     /locus_tag="PLB25_03250"
     CDS             complement(11032..12030)
                     /gene="gap"
                     /locus_tag="PLB25_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019300876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HOE40638.1"
                     /translation="MTIKIGINGFGRIGRNVLRSALQSFSDIEVVAINDLLEPTYLAY
                     MLQYDSVHGRFKGEVSVDGNTLIVNGKKIRLTQERDPANLKWNEVGADIVVESTGIFL
                     DKAGGEKHLAAGAKKVIFSAPSKDDTPMFVFGVNDKTYAGQTIISNASCTTNCLAPIA
                     KVVNDKWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGV
                     VIPALNKKLTGMAFRVPTSDVSVVDLTVELNSPATMAEICAEIKAQSEGAMKGVLGYT
                     EDKVVATDFRSDPRTSIFDADASIALDGTFVKLVSWYDNEWGYSNKCLEMVRVLAK"
     gene            12361..13482
                     /locus_tag="PLB25_03255"
     CDS             12361..13482
                     /locus_tag="PLB25_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004533484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HOE40639.1"
                     /translation="MSLLFSPLYLPCPRGGLQLPNRIVVAPMCQYSANDGCATDWHLM
                     HWGNLLNGGAGLFTIEATAVLPEGRITPNCLGLWDQRTEAALALHLQRARSLAPQMAV
                     CIQLSHAGRKASCAVPWQGGHQLTPDEGGWRTVGPSALPHLPKEAAPSELASDDMAHI
                     VAAFVAAARRAAAIGIEAIELHCAHGYLLHQFLSPLSNQRQDAYGGSFENRVRFPLAV
                     FAAVRAAFDGVLGLRLSASDWVEGGWDLPQSQAFCQLLKAAGCDFIHVSSGGISPEQT
                     MLPGALYQVPFARAIRAGTGMVTTAVGLITDASQAEAILQAGDADLIALARAFLYQPR
                     WGWQAAAALGASVLASPPYWRCLPHQAQAVFGTASTAQR"
BASE COUNT         2879 a         3994 c         4058 g         2613 t
ORIGIN      
        1 acgatttcat cgtttccaag accgacctca agggccgcat cacctactgc aaccgggtct
       61 ttatcgagtt ttcaggttat aactcctccg agctgatcgg gcagcagcac aacatcgtgc
      121 gccatcctga catgccgcgc ggggtcttca agtttctgtg ggatacgctg gaacaaaata
      181 aagagtgctt cgcgtttgtc aaaaacatgt gcaaggacgg cagcttctac tgggtatttg
      241 ccaatgtcac gcccgacctc gatgccaagg gcttggtggt cggctatttt tcagtgcgtc
      301 gcaaggccag tgccggggcc atcgcgctga tgaccgatct atataaggcc atgctcgaag
      361 aagagcgcaa ggtcggcccc aaagacgctt gcgctgcctc gctggccctg cttgccaata
      421 ctttgcagca aaaaggagtc agctatgagc aactcgtcct ctcgctctga atcgtcggct
      481 tatgtttgga ccatggcttt gctgcttgtg gcaggtgccg cactgcaaag ctggcacacc
      541 ggctggagcc cgtttgtgct ggtctggctg ctgcctggct tgttatgtct gttgttctgg
      601 tatctgagct tgcaacgcca acatcggctg atgatggaca ttcagagcat ttcgcagcag
      661 gtggcgcacg gccagttggg ccgtcgcctc gacaaaattc cggccagcgg cttgtttcac
      721 cagttatgct gggatttcaa cgacatgctc gatcagctcg aagcctgctt tcgcgagcag
      781 gccacagcct tgcagtacgc cagcagcgga caatatttca ggctggcgca agcgaccggc
      841 ctgcgtggta cctttggcaa gtcgctggag caaaccaatc agtccctgct ggcgatggcc
      901 cagactgccg cagcagaaca gcaggctgcg gccgaaaagc tggctgcaca ggaaaaagag
      961 cgcgtggtgg ccaacgccaa ccgccgaatt cgcctggcac tcgacaacgt cagcctgccg
     1021 gtgcgtattg ccgacgacaa cggcaccgtg atttacatca acaatgcctt gcgcgacacc
     1081 ctgcatcgca acgcagaggg ttttcgccag cagattcccg ggtttgaccc ggccaaggtg
     1141 gtgggcggca gcatcggcat gttttacgcg gatgcagagg cggccctgac gcgtctgcga
     1201 gatttgactg cggcgacaca aacccgactc aagctgggag gtcgcatgta cgacctcacc
     1261 acaacaccgg tgctgtcgca agaaggcgag cgcctgggca cggtcggcca atggcaagat
     1321 gtcaccgagc aactggcggc tgaagaggaa atcgaccaga tggtgcaagc cgccgcccag
     1381 ggcaacctga gccagcgcct gagcttgcac ggtaaaagcg gattttttgc caatttatcc
     1441 aatggcatga accagttggt gcaaaccacc gaacagggcc tgtcagacgt ggcccaagtg
     1501 ctcagtgccg tggctgatgg cgacctgacc caacgcatca cacgcgacta tgccggcctg
     1561 tttggcaagg tccgggacaa tgtgaacgca tcggccgaga acctgacacg ggtcatgagc
     1621 gaagtccgag cggccgccaa cgcgctgtca gatgcggcca accaggtctc ggccactgcc
     1681 cagtcgctga gccaggcatc aagcgaacaa gccgccagcg tcgagcaaac cacggcatcg
     1741 attgatgtga tgtcggtttc catcacgcaa aacagtgaca acgcgaaagt gaccgatggc
     1801 atggccacca agaccaccaa agaggctgtt gagggtggcg ttgcggtcag tcaaaccgtg
     1861 gtagccatga aacagatcgc atccaaaatt ggtatcgtgg atgacatcgc ctaccagacc
     1921 aacttgctgg ccttgaatgc ggcgattgaa gcggcccgtg ccggtgagca cggcaagggc
     1981 tttgccgtgg tggcggccga agtccgcaag ctggcagaac gcagtcagga agccgccaaa
     2041 gagatcggtg aattggccgg cagtagcgtg atcaccgccg agcgcgccgg tcggttgctc
     2101 gaacagattg tgccgagcat tcaaaaaacc agcgagttgg tgcaggagat tgccgcttct
     2161 tccagcgaac aaagcagcac cgtaaccgaa attggcggtg ccatgggcca gctcagcaag
     2221 gccacccagc aaaacgcctc ggcctctgaa gaactggccg ccaccagcga ggaactctcg
     2281 ggtcaggccg agcaactgca gcaaagcatt gcgtttttca atatcggcga cgatgcccct
     2341 gccgtgcccg accgccatgc cagggcacca aacatgaaaa tgccagcgca gtacccgatg
     2401 ccgcgcctgc gcagcagccc aaaagcgtcc gctcagggca atttcaaacc ctattgaccc
     2461 ccttttatgg caggtatcgc ctcatgagcc aggacaagga catcgaagag cgcacccggc
     2521 tggcgggcac caacaagttt gagttgctgc tgttccgcct gggcgagtca cccagcaccg
     2581 gtgaacgaga gttgtacggc atcaatgtgt tcaaggtgcg cgaggtgctg gtcatgccca
     2641 aaatcaccgc attggtgaac agccacgaac acctgatggg ggtggccaat atccgtggcc
     2701 aaatcattcc ggtgatcaac ctgcccgcag tggtgggctg ccagcccaaa aaaggactct
     2761 ccattttgat ggtcactgaa tttgcgcgca ccatccaggg ctttgcggtt gaagacgtac
     2821 gtgaaatcgt tcggctcgac tgggaccagg ttctgccagc agaaagcagc cacgccgggg
     2881 cgctggtgac tggcattgcc cgcctcgacg gtgcccgcgc cgatacccgg ttagcgcaag
     2941 tgctcgacgt tgaagaagtc ttgcgcgatg tcatgccggt gaccaaagaa gaaatcaacc
     3001 gcgaggtggt gggcccggaa ttgttgcttc cagagggcac ctcgatcttg gtggctgatg
     3061 actcgcatgt ggcgcgagca gtgatcgaac tcggtctgaa ggccatgggg gcaccgtttg
     3121 tgatgaccca cactggtcgc caggcctggg actaccttca agcggcggca gacaaggcca
     3181 aggccgacgg cacgcgcgtc aaagacaaca tctcggtggt gctgaccgac ctggaaatgc
     3241 ccgagatgga tggctttacc ctgacccggc tgatcaagca agatgcccgt ttcaagggta
     3301 ttccggtggt gattcactcg tcgctgaccg gcaccaccaa tgaaaaccac gtcaaaggcg
     3361 tgggtgccga tggctacgtc ggcaagtttt ctccacgcga actggccaac acgctgcgtc
     3421 aggtcttggc gggtcaggtg tcccattgat cgttaactgg agcttatctt gaagtcaaca
     3481 caatactcat ggctggcgcg gatgtttcat ggcgcggaaa tcaatcgccg cctggccgac
     3541 ctggagcagg agcttaaggt ccgcaccgac atcatgaacc tgacttcgat cgtctcggag
     3601 tcagacaaaa agggcgacat ccttagcatc aatgacaagt tcgtcgaggt ttccaaatac
     3661 agcaaagacg aacttttagg ccatccgcac agcaccacgc ggcatccgga catgcccaag
     3721 gaaaccttca ggcagctctg ggcaaccatc ggacgagggg cgatgtttcg aggggttatc
     3781 aagaatcgcg ccaaagatgg cgcaccctat tacgtcgatg cggtgattgc gccgtttatg
     3841 ggcgacaacg gcaagcccat gaaatacctg ggggtccgtt acgacgtcac gcaagcggaa
     3901 accgagcgcc aaaacgccag gggaattctg ggggccgttg acgcgtcttt tggcatggtc
     3961 gaatttgacc tgggtggtca tgtactgcag gcgaatgtca actttttgca gatcatgggg
     4021 tacagcagtg ctgaagtttc gggcaaaaac caccggttgt ttgtcgaggc tagccttgct
     4081 gccagcccgg cataccagca actctgggct gacctgaagg ccggcaaacc acaaaaaggc
     4141 actttcaagc gcgtcggcaa ggggggtaaa gaggtttggt tgcaggcggt ttacagcccg
     4201 gtgatggatg aaatgggacg ggtgttgaag gtggtcaaaa ttgccaccga tgtcagcagt
     4261 gaatttcagg ccaatgccat gctgcagaca gcggtgcatg attcgctggc ggtgatcgct
     4321 gccgccaaag acggagattt gagtcaacgg gtggatttgg gcggcaaggc cggcccgatc
     4381 gcctcgctgt gtaccggtgt caataccttg cttgacacca cggtcatcgc ttttgccgac
     4441 atcggccggg tgctgggtgc catgtcagca ggcgacttga gccagcgggt cacccgcgag
     4501 tatgccggca ccttcaacca gctcaaacag gatgccaatg tcacttccga gaagctttcc
     4561 aacatcattg acgacatggc ccgggtgttt gaaggtctgg ccagcggcga tttgacccag
     4621 cgcataaccc gcgacaccga gggcgcattt gagcgtgtca agcaggatgc caacctgagc
     4681 ttcgaaaagc tcgccgccat catcgaagag gtacgcgccg ccgccgatgc cctgactggc
     4741 gctgccaatc aggtcagtgc cacagcccag tctttaagcc aggccgccag cgagcaggcg
     4801 gcctcggtcg agcagaccac ggcatcgatt gatgtcatgt ccgactcgat cacccacaac
     4861 agcgacaacg ccagggttac cgatggcatg gcgaccaaga ccaccaaaga agcagtggat
     4921 ggcggcagtg cggtgagcca gacggtgacc gccatgaagc agattgccgc caagatctgc
     4981 atcgtcgatg acatcgctta ccaaaccaat ttgctcgcac tcaacgctgc cattgaagca
     5041 gcccgcgccg gcgaacacgg caagggtttt gcggttgtgg cggccgaggt acgcaagctc
     5101 gccgagcgca gccaggaagc cgccaaagag attggtgaac tggccggcaa cagtgtcacc
     5161 accgccgagc gggctggcag actgcttgac gcgattgtcc cgagcatcca gaaaaccagc
     5221 gaactggtgc aggaaatcgc cgctgccagt gccgagcaaa gcgaatcggt gtcgatgatt
     5281 ggcgaggcca tggggcaatt gagcaaggcc acgcaacaga acgccagtgc ctccgaggaa
     5341 ctggccgcca ccagcgaaga gttatccggt caggctgagc agttgcagca ggcagtgggg
     5401 ttctttaacc tgggcggtgc ggccccacag cttcgcagcc gtcatgagtt gcccgcaccc
     5461 gagcggcgtg gcgcgcccga gcgcaagccc gcgcccaggc tggagctacc agggcgcagc
     5521 ggtggcaatt tcaaacccta ttgagcccag tttcaagtta agcaaaaagt gagtgctgaa
     5581 tcatgagtgc gaacccaagc cacggcgctg aagccaaccg cacaaccggt ttttttgcgc
     5641 ccggcatgcg tctgatggga aatctgcaat ttgccggcaa ggcactgctg atttgcttgg
     5701 tacttttgtt gccttttggc tggttgacct gggcgtttta cagcagcaag gccgacagca
     5761 tggcgttttc tgtcaaggaa ctgcttgggg tcagctacaa ccgggagatt tttccgctga
     5821 tcaacctggc ccagcaatgg cggcgcgatg catcatcgct ggcggccaac ggtgaggcac
     5881 cgcccaccct ggcagacgtg catgccaaac tgaaggcggc acaagagaaa ctggcgactg
     5941 tagacaaggc attgggtgcc caactgaaca cggccaaacc gttcgaagaa gttcaaaggg
     6001 cttttgccca ggcgcaggcg gccagcgggt cagtggaccg ggtgtttgac gctcactcgc
     6061 aacacatcaa ggcactgatc aacttgatga cgcaggtgac agatgcctcc aacctgacac
     6121 tggaccccga tattgactcc tactacctga tggacgccag ctatttcaga atgcccgacg
     6181 ttctggagag cagtggcaag ctgcgcggca tgggactggc catcatgaaa gccagagcca
     6241 tcaccgccgc gcaacttgat acgctcaacg cgctgatgcc aattgccatg tttcagttca
     6301 acaacatgcg cggcggtctg gccaagtcct acgccttcaa cccggcattg gcctcgcagc
     6361 aagaggcagc aaacaccttt ggcatcaccg agtcgttttt tgcgctggca cgcaacgggg
     6421 tcattgatgg caaggactac acagcagaaa cccagcagtc ttacctggcg ctgggaaaca
     6481 tggccatcga cgcgcaattt gccttgctca acgtgttgca agacgaactc aatggcctgc
     6541 tcagcaagcg ggttgaggcg atgcgcagcg cactcatcgt caccacagtg atcctgctgg
     6601 tgggcttggc actggtgacg tatttgttct acagcttttt tctggtgacc cgtggtggcc
     6661 tcaataccgt caacacaacc ctgcatcaga ttgcccaagg tgacctgtcg cacacgccag
     6721 agcaaccttc gggacgtgat gagccagccc aggtattgcg ggcggtgatt gacctgcagg
     6781 cagtgctggc gcagtttcgc gcctcgcagg ccgagctggc gcgccagcac gcggcgggta
     6841 tgctcgattt tcagatgtcc acccaaggcc tgcccggaga ctatgcggcc atggctgatt
     6901 ccaccaacca gttgctcaac gcgcacattg ccgtcaagat gaaggtggtc gaggtcgtta
     6961 ccgcttacac cgagggccac ctggaagtcg ccatggaccg cctgccgggc cagaaggcgc
     7021 gggtgaccga ggccatcgac aaggtgcagg ctgccatgaa agctgccgcg gcagcggccg
     7081 cgtttaacca gcgcatccgc ctgtcgcttg acagcttgcc ggtgtgcgtc accatttcgg
     7141 atgcccaagg ttttctggcc cacgccaccc cggctgcgca tcagttgctc aagctgtttg
     7201 gtggtgagag cttcgaggcc gacaagtttt atggcaacaa gctcagcacc ttgttcaaaa
     7261 acccggcaga tgccagccgc tttgaccagg ccgtgttgtc tggtgaaacc gtggacatgg
     7321 agatacaggg ccgcaaactg cgcctgttgg cccgaccggt gcagggtagc cggggtactg
     7381 ccctgggccg catcacccaa tggctggacc gcactgatga gatcgccgcc gagcaggaac
     7441 ttgacaacat ggtcaacgcc gccacgcagg gcgacttcag tggtcgcttg agcgtggccg
     7501 gtaaaaccgg ttttctgagc aaaatatcag aaggcatgaa ccaactggtg gccacctgcc
     7561 agcagggtct gtcggatgtg gcgcaggtgt tgtcggccgt tgcccaaggt gacctgaccc
     7621 agcgcatgac gcgcgactat caaggacttt ttggcaccgt caaggacagt gtcaacacca
     7681 gcagcgacaa cctgacccgg gtcattggtg aagtgcgtgc tgccgccgat gcactgaccg
     7741 gtgcggccaa tcaggtgagc accactgccc agtcgctgag ccaggcggcc agcgagcagg
     7801 cagccagcgt tgagcaaacc tcggccagca tcgacaccat gtctgcctcg atcaaccaga
     7861 acagcgacaa cgccaaggtc accgatggca tggccaccaa ggcgaatcaa gaggccgttg
     7921 agggtggaac cgccgtcagt cttactgtgg cagctatgaa gcaaatagca tcaaagattg
     7981 gcattgtcga tgacattgcc taccagacca acctgctggc actcaatgct gcgatcgagg
     8041 cggccagagc gggcgagcac ggtaagggtt ttgcggtggt ggccgccgaa gtacgcaagc
     8101 tcgccgagcg cagccagcaa gcggccaagg aaattggtga gcttgccgcc aacagcgtga
     8161 ccaccgcaga acgggctggc aaactgcttg atgaaattgt ccccagcatc cagaaaacct
     8221 ctgagctggt gcaggagatc gccgctgcca gcagcgaaca aagcgaatcg gtcacgcaaa
     8281 ttggcagtgc catgggccag ctcagcaagg ccacacagca aaacgcctct gcctcagaag
     8341 agctggccgc gaccagcgag gaactgtcgg ggcaggctga gcaattgcag caaagcatcg
     8401 cgttctttga caccggtgac ggcgcgcatc cagtacctaa ccgccacgac cggccgacac
     8461 ccacgccgcg cagcaatgca ggcaggttac ccggtcgcag cgctgcaccg gtgctgaacg
     8521 cgccagtgcg cggcagtggc aatttccgcc cctactaaga aaggtctgtg tgtctgccat
     8581 caccttcagc gaccgtgagt tcaaacgcat cagcgagatc atgtacgagg ctgcaggtct
     8641 gtccttcaat gactccaaaa agccattgat ccagtcacgc ctggcaccgc gcatccagcg
     8701 catgggcctg gccagctttg gcgattacat cgctttgcta gaagacgagt cacaggcagc
     8761 cgagtttcag atggcggtgg acatgctgac caccaacgaa acctattttt ttcgcgaacc
     8821 caggcactat gacttgctgg agcaggaact gaccgcccgc aagagtcgcg cgccgctggc
     8881 tatctggagc gcggccgcct cgtttggtga tgaggcctac agcaccgcca tgctgttgtc
     8941 ggacatgcag gtgcaagggc gtgtcgggcc ggactggtcg attctggcaa ctgacatcag
     9001 ccaccgggtg ttgctcagcg ccaaagaggc ggtgtacccg gcggaccgcc tgcgtgccgt
     9061 cagcccggag cggctcaaac gctattgcct gcgcggggat ggcgaatccg aagggcaggt
     9121 tctgattcag gacaagattc gccagcgggt gcaatttggc cgcttgaatt tgtgccagcc
     9181 cattgatggc gttgggccgt ttgacatcat ttttttgcgc aatgtgctga tttactttga
     9241 catccccacc aagattgagg tggtggaccg ggtgctgacc accttaaggc ccggtgggct
     9301 gttttttttg ggcacggccg aaggccgggt ggcctgcaag acaccgctgt taaccgtgca
     9361 gcccggtgcc tttcgcaagc cagcctgatt cggacgacca agcatggcta cttttgaact
     9421 gatgcccggt gacgtggcac ttggttacgc tggtgaccag cttaagacct tgctgggctc
     9481 gtgcgtcagc gtgattttga ccgacccgcg tcgcaccgtg ggcgtgatgt gccacatcgt
     9541 ccacgtaggc catcccaaca ccgccaaccg gaacaatacc gcctacggct cggtagccat
     9601 ggccgacatg acccgtcgcc tttacgccat tggctttgcg ccgcgcagtt gccaggccta
     9661 tgtggttggc ggcggcaata tgtttcccaa gctgttcact caccaccatg tgggtgccag
     9721 caacatcgac tgggtgctgg gttatctgga acaccataag attaaagtac tcaggcaaga
     9781 cttgggcggc actggctatc gcaagctggt ctggaccgtc ggtggcagcg atccgacggt
     9841 tgaaaccgcc tcttctaact aacaggaaac cagacaatgg tcgtcaaagt atttgtggtc
     9901 gatgattccg ccgtggtgcg ccaggcgctc gcgcacatgc tggcaggcaa ctcagatgtt
     9961 gagctgattg gcagtgcgcc caaccccctg ctggccatgg cagccattcg caaaaacccg
    10021 cctgatgtgt tgctgctcga cattgaaatg cccggcatgg acgggttgac cttcttgcgc
    10081 cagatcatgg ccggcacgcc gattcccaca gtgatttgct cgacgctttc caccgagggc
    10141 agccgggttg cgcttgatgc gttgagcgcc ggtgccgtgg cggtgctggc caagcccaaa
    10201 ctcgggctca agcagcatct cgatgattcg cggctggaga tgatccaaac cctgaaaacc
    10261 gctgcccggt ctcggccacg tgcccgaggc gctatgcctt ctatagctgc taaggcaatt
    10321 acgacggggg ttgcagccca aataggccgc atgccagcac caagcggtgt acatgccttg
    10381 gcgatgaaca agccggtggt gattggcagc tcgaccggtg gcacgcaggc gctggaactg
    10441 gtactgacca gcctgcccgc tgatgcgccc ggcattgcca ttacccagca catgccggaa
    10501 aaattcaccg ccatgtatgc gcagcgcctg gacggcttgt gcgccatgaa tgtgcgcgag
    10561 gccaaagacg gcgaccggct ggagcgcggc gtggcgctga tagcccccgg tgggcgtcac
    10621 atgcagttgc gaaaaacgaa cggccagtat tttgtcaagg tgctcgacgg ccccccggtc
    10681 aaccgccaca agccgtcagt tgatgtgctg ttcaagtcgg ctgccgaatg cgccgggcgc
    10741 gacgcgctgg gcatcatcat gaccggcatg ggcgacgacg gtgcccgcgg catgaaagtg
    10801 atgcatgacg gtggtgcccg cacgattgcc caaaacgaag aaacctgcgt ggtgtttggc
    10861 atgcccaagg aagccatcaa gctacaggcg gtcgatgaca tcttgccgct cgaaggcatg
    10921 gcccgggcga ttctgcagtt tgattcgcgc gcctgaggcg gctggccggc aatgaaaaag
    10981 cccgcgctgc cgggcagcgc gggctttggg agaaccggga aaaagcgctg cttacttggc
    11041 cagcacgcgt accatttcaa ggcacttgtt ggaataaccc cattcgttgt cgtaccagct
    11101 taccaatttg acaaaggtgc catcaagcgc aatgctggca tcagcgtcaa agatggaggt
    11161 gcgggggtca ctgcggaagt cggtggccac caccttgtct tcggtgtaac ccagcacgcc
    11221 cttcatggcg ccttcgcttt gggccttgat ttcggcacaa atttcagcca tggtagccgg
    11281 tgaattgagt tcaaccgtca ggtcaaccac tgacacgtca ctggtgggaa cccgaaatgc
    11341 catgccggtg agcttcttgt tgagagccgg aatgaccaca cctacagcct tggcagcgcc
    11401 agtgctcgaa ggaatgatgt tttccagaat gccacggcca ccgcgccagt ctttgttgct
    11461 tgggccgtca accgtctttt gcgtggcagt agccgcgtgc acggtggtca tcaggccgcg
    11521 cttgatgccc catttgtcat tcaccacctt ggcgatcggg gccaggcagt tggtggtgca
    11581 ggacgcattg ctgatgatgg tctgaccagc gtaggtcttg tcgttcacgc caaacacaaa
    11641 catcggggtg tcgtctttgg aaggagccga gaagatgact ttcttggcgc ccgctgccag
    11701 gtgcttttcg ccaccggcct tgtccaggaa aatgccagtg gactcaacca caatgtcagc
    11761 accgacttcg ttccacttca aattggccgg gtcgcgctct tgcgtcaggc gaatcttctt
    11821 gccattgaca atgagggtgt tgccatcgac tgacacctca cccttgaaac gaccgtgcac
    11881 cgagtcgtat tgcagcatat aggccaggta ggtgggttcc agcaagtcgt tgatggccac
    11941 gacttcaatg tcgctgaagc tctgcagggc agagcgcaaa acgttgcgtc cgatgcggcc
    12001 aaagccgtta atgccaatct tgatcgtcat atttttctcc agaagttaca caaaaaaaac
    12061 caatcacttg tctcacgcgg cctctactgt atcagccctg ttgccggcac ttgcgccccg
    12121 gtactgaaac accatcggca tcttgattgc cgtgccgctg gcgcgattgc ccacaacacc
    12181 ggcggcacat gctggtttgc agccttttgg ttcagaccag cccagcgcac cggacacctt
    12241 gaagaaccaa aagactgtca acgtcagaca ttctaatggt cggtttggcg tgccagactg
    12301 acatcgggct ggccccaaca agcagcttcg ccgatacgcg tttgggctat gcttgatgcc
    12361 atgagtctgc tgttttctcc cctgtacctg ccctgtccgc gcggcggact gcagttgccc
    12421 aaccgcattg tcgtggcccc gatgtgccaa tactcggcaa acgacggctg cgctaccgac
    12481 tggcatctga tgcactgggg caatttgctc aacggcgggg ccggtctttt caccatcgag
    12541 gccacggctg tgctgcccga gggtcgcatc acccccaact gcctgggttt atgggaccag
    12601 cgcaccgaag cagcgctggc cctgcatctg cagcgcgccc gctcgctggc accgcagatg
    12661 gcggtgtgca ttcagttgtc gcatgccggg cgcaaggctt cctgcgccgt gccctggcaa
    12721 ggcgggcatc agttgacccc ggacgaaggc ggctggcgca cggtgggtcc gtcagcgctg
    12781 ccacatttgc cgaaagaagc cgcacccagc gaacttgcca gcgatgacat ggcgcacatc
    12841 gttgcggcat ttgttgcagc ggcccggcgc gccgcagcca tcggcatcga ggcgattgaa
    12901 ctgcactgcg cccatggcta cctgctgcac cagttcttgt cgccactgtc taaccaacgc
    12961 caggatgcct atggcgggtc gttcgaaaac cgggtgcgct ttccgctggc ggtatttgcc
    13021 gccgtgcggg ccgcctttga tggcgtactg ggcttgcgct tgtcggcctc ggactgggtc
    13081 gagggcggct gggacctgcc gcagagtcag gcgttttgcc agttgctcaa ggctgccggt
    13141 tgcgacttca tccatgtgtc ttccggtggc atctcgcctg agcaaacaat gctgccgggc
    13201 gcgctttacc aggttccctt tgcgcgtgcc atccgggctg gcaccggcat ggtgaccacc
    13261 gctgtcgggt tgatcaccga tgccagccag gccgaagcca tattgcaggc aggcgatgct
    13321 gacctgattg ccctggcgcg ggcatttttg taccagccgc gctggggctg gcaagctgcc
    13381 gcagcactgg gcgcaagcgt gctggccagt ccgccgtact ggcgctgttt gccacaccag
    13441 gcgcaagctg tgtttggcac ggcatcaacc gcgcaacgct gaagcaccca acgccatgca
    13501 cggcctgcac ctgaccgctg atctgcatca atgtggctgt gagc
//
LOCUS       DAOSLL010000045        13322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4728_length_13322_cov_5.144795, whole genome shotgun sequence.
ACCESSION   DAOSLL010000045 DAOSLL010000000
VERSION     DAOSLL010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 13322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13322
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4728_length_13322_cov_5.144795"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PLB25_03260"
     CDS             complement(<1..461)
                     /locus_tag="PLB25_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HOE40640.1"
                     /translation="MRIISLHGISVSETSVSDFQQALDNLRLPDQGYLWLACSRSEFG
                     QMQVPIQAMLDRLCGLQLMELHVSDLLNQQLPSRYDYTSQYDLLVFRRLAASVASAQP
                     PSDATLESSAKRRGPPILRQIDTSPVGFVVFDRVLLTVHPQEDATRDSYALR"
     gene            1151..1438
                     /locus_tag="PLB25_03265"
     CDS             1151..1438
                     /locus_tag="PLB25_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HOE40641.1"
                     /translation="MPSQLPTDNPSPSLITYPCQFPIKVMGRNVDGLVAAISHIARQF
                     DPGFDASTIELRPSKAGNYLGITLTINATSREQLDEVYRTLSTHPMVKVVL"
     gene            1441..2139
                     /gene="lipB"
                     /locus_tag="PLB25_03270"
     CDS             1441..2139
                     /gene="lipB"
                     /locus_tag="PLB25_03270"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HOE40642.1"
                     /translation="MQVQRLGRADYLPTYEAMQAFTASRTANTPDELWICEHSALYTQ
                     GLAGKNEHIFSPGAIAVVQTNRGGQVTYHGPGQVVAYPLIDLKRAGYFIKEYVYRIEE
                     AVIRALMHFGVTGHRVAGAPGIYVRLDDPRSHALLAQRPMKLGPADAAAAKSPDFTGL
                     GKIAALGIKVSRNCTYHGVALNVAMDLEPYSRINPCGYPGLQTVDLSTIGIFVSWQDA
                     ADIFAQKLTSYLAP"
     gene            2254..3252
                     /gene="lipA"
                     /locus_tag="PLB25_03275"
     CDS             2254..3252
                     /gene="lipA"
                     /locus_tag="PLB25_03275"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HOE40643.1"
                     /translation="MSTSDSGTPVQREVQAHYDPTVKQKAGAKLSRIPIKVQPGEILR
                     KPAWIRVKAGSPNTRFYEIKDVLRSNKLVTVCEEASCPNIGECFGKGTATFMIMGDKC
                     TRRCPFCDVGHGRPDPLDANEPVNLARTIAQLKLSYVVITSVDRDDLRDGGAGHFVAC
                     IEQIRQLSPHTQIEVLVPDFRGRDDRALDILKAAPPDVMNHNLETIPRLYKEARPGSD
                     YLFSLNLLKKFKAQFPDIPTKSGLMVGLGETDDEILQTMQDMRDHGINMLTIGQYLAP
                     SSSHLTVKRYVHPDVFQMFETRAYAMGFSHAAVGAMVRSSYHADQQAGHAMGRTVL"
     gene            3310..6789
                     /locus_tag="PLB25_03280"
     CDS             3310..6789
                     /locus_tag="PLB25_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE40644.1"
                     /translation="MQTHWQSSNLWLDLASLVQKCDSTCYARGLELYRNQRVLNLSIE
                     PLKGYWLLMGDVQGSARHPYEVSIEVRRNVNGRVLEWDSDCTCPVGYQCKHGVALMIK
                     AAYKGAQLLGTAGPKAASRPATPQELDALREAELARQQAAAQLDAENQVLRWLGELER
                     SSPQTPDPLHAQASPQRLQSPAIKHEYFLYLLTMSGLTGSTPHLDLEVVLSYEKQSGG
                     WAKSKVIKYQPERGQAVFDLATEADHDVLQLMRAMPSSSSSYYYNYGVKTKVTIEGKY
                     GVQALQQAASTGRLFIGDGKSQPVTPAQWGPALEVRWQWHEVTQASTGDSGWVLRPQL
                     DEPQAKLCLNNPPLYLDSQRGVCGLAQVSGMGLGQVAVLLKSPPLKSEALKKHQLALA
                     ERLGSLPLPPVLEQLVTLTGISPIACLHLSPVLPQRIPEDGLMQAKMTFDYNGHRGWW
                     VGQGNTVVVTEGKARCLLQRDPQTELEAIMRLRELGLASDGRGHFGIPGHGPQQIWLQ
                     WVDSDYAPLRQAGFELTLDDALTDWISRADTLNVNLAARGDDEATSPWFDLSLGMEIN
                     GQRHNILPWLPELIAVAAGSPPNAATGLPELPEFVYLPAPNGGFIRLPTEPLKPWMAA
                     LLELVGDREHDFSGDSLKLSRFDALRTTASLGEGAVWAGSSHLREMVQRLSGRSELPE
                     VVLPKSVNANLRPYQQQGVNWLQFLREYELAGILADDMGLGKTLQTLAHIQIEKDAGR
                     LTLPALIIAPVSLMGNWRRETERFCPDLRCLVHHGLDRHEAADSMAEYDIVIAPYSLL
                     HRDRDRWLQAKWHLVVLDEAQNIKNANTNAAQVASELQSRHRLCLSGTPMENHLGEIW
                     SLFHFLMPGFLGSQKKFTEIFRHPIEKQGDPEALHQLRARVTPFMLRRTKALVAHELP
                     PKVETVMRVELSGKQADLYETIRLGMEKTVREALSTKGLAKSQITILDALLKLRQVCC
                     DPHLLKLDAAKKVKVSAKLEQLMAMLPEMLAEGRRILLFSQFTSMLSLIEDELIKRNI
                     KWVKLTGQSQKRDDLITQFTSGAVPLFLISLKAGGVGLNLPQADTVIHYDPWWNPAVE
                     NQATDRAHRIGQTQSVWVVKLVAQGTIEERILALQDPKAALAESMYSGSVGRKQPLFS
                     ESDLAELLKPLSV"
     gene            7138..8181
                     /locus_tag="PLB25_03285"
     CDS             7138..8181
                     /locus_tag="PLB25_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40645.1"
                     /translation="MRNDESTVRRLVSARTGIAAALAFGLVGCGSLPDAKVNYYQTTS
                     QITVKVTRSVLCDTKNFPVVANVAAPNVTHSADLTKPRSTDLAGLRGSFTDADIKFDL
                     YEDGRLKAVNATQTGQGDAVVKAATTLATTIAAFSTTSTLPSYPTECTFIKDVGGGKP
                     LALSYEGVLNLSTKDSQNIPADGASAAYASRLSAFLGGICAKVGETEVPAKLVEYTGS
                     DAVVTATAPAMVWVEVGTARPGNLCVPDLWKGRLPVAQLGKEYKIPLPNAVVFGKQTL
                     SLAFAESGAVTSMQYTTASGAAGALGAVNALATIAQPESTTAKTTDLKSQADEIVQEQ
                     RLLQCKADPKNCK"
     gene            8215..9618
                     /locus_tag="PLB25_03290"
     CDS             8215..9618
                     /locus_tag="PLB25_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HOE40646.1"
                     /translation="MAHRILTLFALLLIAAPALAVDPVVITLVSDGAVTAKPGSGSTV
                     VERGKATATAKADGDEDIESCERYGRRSSANGRAAVTTVSAASPTSLSYRLDAAASAS
                     GGHYRTGTCIADRRIGFTGHDTEAKVDAVATAVVKIHFNGGRPNVPYFVKISRSQSGT
                     AQNDQLTGPDGTVIPLNDPKSPHPVILSKPGQDYFLRTTVAATAHNGGGCCSDQMSAI
                     SDMSVFIEPAPLLFAAGQSPFIAKGFQTTGYKNVVVIMLEGLPHCTATMVSPTVLVTA
                     AHCVKDHMTLDKMSTGKVLAVFGSGYAQPLFAPVQIVRASYPDTGEQAFDASTLKNDI
                     AVAYLKIAITTPGVNPAPLHTGTPSWQQIKTNKTHLTFVGFGYDVVGGEKTGVGVKRE
                     ASWAITDYTDSIVSFQTDGTSTCNGDSGGPAFLNVNQTLVLAAITSGGDDNACTYGWD
                     TRIDAYLDWLKPKIASE"
     gene            10027..10281
                     /locus_tag="PLB25_03295"
     CDS             10027..10281
                     /locus_tag="PLB25_03295"
                     /inference="COORDINATES: protein motif:HMM:NF019540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOE40647.1"
                     /translation="MKQRHQRTVELIFSRPTSANVQWRDVEGLFVELGAVIEERSDSR
                     VSVFLFNEVRVFHRPHPSPNTDKGAVASIRKWLEQHGVEP"
     gene            10287..10607
                     /locus_tag="PLB25_03300"
     CDS             10287..10607
                     /locus_tag="PLB25_03300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018060192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOE40648.1"
                     /translation="MTVDDFHATIAFDPDLDMFRGEILGLNGGADFYGKNPDELRAEF
                     KRSLEVFLEVCTEKGIEPRRHFSGKFNLRISPELHEKLAIAAQARGKSINALAQEALQ
                     DSFA"
     gene            complement(10847..11239)
                     /locus_tag="PLB25_03305"
     CDS             complement(10847..11239)
                     /locus_tag="PLB25_03305"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOE40649.1"
                     /translation="MLALDTHVLVRLVTDDDPAQAQRMQEALDVELAAGRECLVGHIV
                     LCELIWVLGCLYDHSQAQCQQTMTILLGFVGLRFESMPTVVAAFKVWQREGGDWADHL
                     IGAQMKALGCEAVLTLDHSVPVVKRRIA"
     gene            complement(11226..11420)
                     /locus_tag="PLB25_03310"
     CDS             complement(11226..11420)
                     /locus_tag="PLB25_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40650.1"
                     /translation="MADFTIGKSTVHILLKLRGILPQPKQAGTVEEMNQAVQDAATEK
                     TRSAKPVTPSSTPGYPNAGT"
     gene            11941..12801
                     /locus_tag="PLB25_03315"
     CDS             11941..12801
                     /locus_tag="PLB25_03315"
                     /inference="COORDINATES: protein motif:HMM:NF015709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HOE40651.1"
                     /translation="MTRLRHFPLTALLSGVLVLASVMPATAQTAAAAPNTTGPSGSGV
                     NNALLYAAAWKQTAAEYRALYYQGFNVARMHVEMALSKRKAGDKPLAVVTDMDDTVLH
                     ALNYWGHLIKQNKDYFDDLIWDKWIPENKVTASPGSLDFLKFCAENGVEVFYVTSRDQ
                     GEKTYEYAMGHLKVLGFPYADEKHLTVLRDTSNKEKRQDEIMKDFSVVVFLGDNLNDF
                     RRKYYIKSNVDERLKMMETDREKYGRNYIVFPNPTDGHWLAAIFGESEPPPTDANREI
                     MKKAATKSAW"
     gene            12908..13322
                     /locus_tag="PLB25_03320"
                     /pseudo
     CDS             12908..13322
                     /locus_tag="PLB25_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008646539.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
BASE COUNT         2848 a         3940 c         3905 g         2629 t
ORIGIN      
        1 cgcagcgcgt agctgtcacg cgtggcatct tcttgcggat gcaccgtcag cagcacacgg
       61 tcaaacacca caaaaccgac tgggctggtg tcgatctgcc gcaatatggg cggaccgcgg
      121 cgtttggccg acgactcaag cgtggcgtca gacggcggtt gcgcactggc gaccgaagcc
      181 gcgagtcggc gaaacaccag caagtcgtac tgcgaggtgt agtcgtagcg cgagggcagt
      241 tgctggttga gcaggtctga cacgtgcaat tccatcagtt gcagaccgca cagccgatcc
      301 agcatcgcct gaatcggcac ctgcatttgc ccgaattcgc ttctgctgca ggccagccac
      361 aggtaaccct gatcgggcag gcgcaaattg tccagggctt gctgaaagtc actgacactg
      421 gtttccgaga cactgatgcc gtggagcgaa ataatgcgca tattggtgtg aatcgttgtt
      481 cttatgccat ttctaaacca tccatgccgt tgttatcgcg ccttgcgtgt gcaatgtcgg
      541 cggcagggtg acctggagcc tgtcgggctt ttggcgcatc ggctgcgaac cgaccacggc
      601 agccgatgtg gtaccggtac tttggcccat aaccgggcca tgcgcctgca aatccggcac
      661 ccctcgctgg ggtcgcttct cgctggtcga tccacaagcc cgacagactt caagacacga
      721 atgatataga aatgcaatgc catgaaaacc gggcgctggc agccggtaga gcctgtgaat
      781 tcaaaaattt gccctggcat cgtctgcggc ttctcgcgga aataagttcg accccggcgc
      841 ggccaaccat gtggtggatt tgcagggcct tggcctggtt gtgtgggtga agatgccggt
      901 gcaaaacggg ctgctgcggt cggtttgcag ccgctgcgcg acaggtcctg ccggctcctc
      961 gtcggacaaa actcgccggt gtcttgccgg gtgcggcttt ctggctcaag tcggcggcga
     1021 cccttttgga tatggtcgaa aatcagtcac ccatttggcc gctggatttg agatgcagtg
     1081 cgcccacacc cgacgtgcca gatgggaaag ccatggacga ccatgtcctc agatccattt
     1141 actgccaccc atgccaagcc aattgcccac tgacaaccca agcccgtcgc tgatcaccta
     1201 cccctgccag tttcccatca aggtgatggg ccgcaacgtc gacgggctgg tggctgccat
     1261 cagccacatc gcccggcagt ttgaccccgg ctttgatgcc agcaccattg aattgcggcc
     1321 cagcaaagct ggcaactacc tgggcatcac cctcaccatc aacgccacca gccgcgagca
     1381 gctcgatgag gtctatcgca ccttgtcaac gcacccgatg gtcaaggtgg tgctgtagcc
     1441 atgcaggtgc agcggctggg ccgcgcggac tatctgccga cctatgaggc aatgcaggca
     1501 ttcaccgcaa gtcgcaccgc caacaccccc gatgaactat ggatttgtga gcactcagcg
     1561 ctttatacac aagggctggc gggcaaaaat gaacatattt tcagccctgg tgcgattgcc
     1621 gtggtgcaaa ccaaccgggg cgggcaggtc acctaccacg gccccggaca ggtggtggcc
     1681 tacccgctga tcgacttgaa acgggctggc tatttcatca aggaatacgt ttaccggatc
     1741 gaagaggcgg tcatccgcgc gctgatgcac tttggcgtga ccgggcaccg ggtggcgggc
     1801 gcgcccggta tttacgtgcg gcttgatgac ccgcgctcgc atgcgctgct ggcgcagcgg
     1861 ccaatgaagt tggggccagc cgatgccgct gcggccaagt cgcccgactt caccggcctg
     1921 ggcaagattg ccgctttggg catcaaggtc agccgcaact gcacctacca cggggtggca
     1981 ctgaacgtgg cgatggacct ggaaccctat tcccgcatca acccttgcgg ctatccgggt
     2041 ttgcagaccg ttgatctttc tacaatcggt atttttgtct cgtggcaaga cgctgcagac
     2101 atttttgcgc agaagctgac cagttacctg gccccctgac tcgcaagtta tttgatagcc
     2161 gttcgggctg agcctgtcaa agcgcatcgc atgagcacag ttcagctcat gatgaccagc
     2221 ccgttgggaa tttttgggca aggaaccaag cacatgagta catctgattc aggcacacca
     2281 gttcagcggg aagtgcaagc acactacgac ccgacggtca agcaaaaggc cggggccaag
     2341 ctgtcgcgca tcccgatcaa ggtgcagccg ggtgaaatcc tgcgcaaacc ggcctggatc
     2401 cgcgtcaagg ccggcagccc aaacacccgc ttttacgaga tcaaagacgt gctgcgtagc
     2461 aacaaactgg tgaccgtttg cgaggaagcc agttgcccca acattggtga atgttttggc
     2521 aagggcaccg ccaccttcat gatcatgggt gacaaatgca cccggcgctg cccgttttgc
     2581 gatgtcggcc atggtcgccc agacccgctc gatgccaatg agccggtcaa cctggcgcgc
     2641 accattgcgc agctcaagct gagctatgtg gtgatcacca gcgtcgaccg cgatgacctg
     2701 cgcgacggcg gggccggcca ttttgttgcc tgcatagagc aaatccgcca actgtccccg
     2761 cacacccaga tcgaggtgct ggtgcccgac ttcaggggcc gcgacgaccg tgcgctcgac
     2821 atcctcaaag ccgcgccgcc tgacgtgatg aaccacaacc tggaaaccat tccaaggctc
     2881 tacaaagaag cccgccctgg ctcagactat cttttttcgc tcaacctgct gaagaaattc
     2941 aaggcacagt ttcccgatat tccgaccaaa agcgggctga tggtcggact cggtgaaacc
     3001 gatgacgaaa tcttgcagac catgcaggac atgcgcgacc acggcatcaa catgctgacc
     3061 attggccagt acctggcccc cagcagcagc cacctgacgg tcaagcgcta tgtgcatccg
     3121 gatgtgttcc agatgtttga gacgcgtgcc tacgccatgg gattcagtca cgcggccgtt
     3181 ggtgccatgg tgcgatcaag ctatcacgcc gaccagcagg ccggtcacgc gatgggccgc
     3241 acagttctct gattgctctg aaataggtag ctggtagcgc aacactgaca agggctggag
     3301 gccaatttca tgcaaaccca ttggcaaagc tccaatctct ggcttgatct ggcatcgctg
     3361 gtgcaaaaat gcgacagcac ctgttacgcc cgaggcttgg agctgtaccg caaccagcgc
     3421 gtgctcaacc tgtcgattga gccactgaag ggctattggc tgttgatggg cgatgtgcag
     3481 ggcagcgcac gccaccccta tgaagtctcg attgaagtca ggcgcaacgt caatggccgg
     3541 gtgctggaat gggacagcga ctgcacctgc ccggttggct accagtgcaa acacggtgtc
     3601 gcgctgatga tcaaggcggc ttacaaaggc gcgcaactgc tgggcacggc tggccccaag
     3661 gcggcttcca ggcccgccac cccgcaggaa ctcgacgcgc tgcgcgaagc cgaactggcg
     3721 cgccagcaag ccgcagcgca gcttgatgct gaaaaccagg tgctgcgctg gcttggcgag
     3781 cttgagcgca gcagcccgca aaccccggac ccgctgcacg cgcaggccag cccccagcgg
     3841 cttcaaagcc ctgccatcaa gcacgagtac tttctgtatt tgctgaccat gtccgggctg
     3901 accggcagca ccccccatct ggacctggag gtggtgctct cctacgaaaa gcaaagcggc
     3961 ggctgggcca agtccaaggt catcaaatac cagcccgaac gcggccaggc cgtctttgac
     4021 ctggccaccg aggcagacca tgacgtgctg caactgatgc gcgccatgcc cagcagttcc
     4081 agcagttatt actacaacta tggtgtcaaa accaaggtca cgattgaagg caaatacggc
     4141 gtgcaggcct tgcagcaggc ggccagcacc gggcggctgt tcattggcga cggcaaaagc
     4201 cagccggtca cgccagcgca atgggggcca gcgctggagg tgcgctggca gtggcatgag
     4261 gtcacccagg catcgaccgg cgacagcggc tgggtgctgc gcccccagct tgatgagccg
     4321 caggccaaac tgtgcctgaa caacccgccg ctgtacctcg acagccagcg tggcgtgtgc
     4381 ggcctggcac aggtcagcgg gatgggcctg ggccaggtgg cggtgttgct gaaatccccg
     4441 ccgctcaaat ccgaagcgct gaaaaaacac caactggcgt tggccgagcg gctcgggtcg
     4501 ctgcccctgc cgccggtgct ggagcaactt gtcaccctga ccggcatcag cccaatagcc
     4561 tgcctgcacc tgagcccggt gctgccccag cgcatccccg aggacggttt gatgcaggcc
     4621 aaaatgacct ttgactacaa cggccaccgg ggctggtggg tcggccaggg caacaccgtg
     4681 gtggtcactg agggcaaggc gcgctgcctg ctgcagcgcg acccgcaaac cgagcttgaa
     4741 gccatcatgc ggctgcgcga actgggcctg gcttccgatg gccggggcca tttcggcatt
     4801 cccggccatg gcccccagca aatctggctg caatgggtcg acagcgatta cgcgccgttg
     4861 cgccaggccg gttttgagct gacgctggac gatgcactga ccgactggat cagccgtgcc
     4921 gataccctca atgtgaacct ggccgcgcgt ggtgatgacg aagccacctc gccctggttt
     4981 gatctgtcgc tgggcatgga aatcaacggc cagcgccaca acatcctgcc ctggctgccc
     5041 gaactgattg ccgtggccgc aggcagcccg cccaacgccg caaccggcct gcccgaactg
     5101 cccgaatttg tctatttgcc cgcgccgaac ggtggcttta ttcgcctgcc gaccgagccg
     5161 ctcaaaccct ggatggccgc gctgctggaa ctcgtcggtg accgcgagca cgatttttcc
     5221 ggcgacagcc tgaaactgtc gcgctttgac gcgctgcgca ccaccgcctc attgggcgaa
     5281 ggcgcggtct gggctggcag cagccacctg cgcgagatgg tgcagcgcct gagtggccgt
     5341 agcgagctgc ctgaagtggt gctgcccaaa agcgtcaacg ccaatctgcg cccgtaccag
     5401 caacaaggtg tcaactggct gcagttcctg cgcgaatacg agctggccgg cattctggcc
     5461 gacgacatgg gcctgggcaa aaccctgcaa acgctggcgc acatccagat cgaaaaagac
     5521 gcgggtcgcc tgaccctgcc cgcgctgatc attgcgccgg tcagcctgat gggcaactgg
     5581 cggcgcgaaa ccgagcgttt ttgccccgat ctgcgctgcc tggtgcacca cggtctggac
     5641 cgccatgagg ccgcagacag catggcagag tacgacatcg tgatcgcgcc gtattcgctg
     5701 ttgcaccgcg accgcgaccg ctggctgcag gccaaatggc atctggtggt gcttgatgaa
     5761 gcgcaaaaca tcaaaaacgc caacaccaac gccgcccagg tggccagcga actgcagtcc
     5821 cgccatcgcc tgtgcctgtc gggcacaccg atggaaaacc atctgggcga aatctggagc
     5881 ctgtttcact tcctgatgcc gggctttctg ggcagccaga aaaaattcac cgagattttt
     5941 cgccatccga ttgaaaaaca gggcgacccc gaagcgctgc accagttgcg cgcccgcgtc
     6001 accccgttca tgctgcgccg caccaaggcg ctggtggcgc atgagctgcc acccaaggtg
     6061 gaaaccgtga tgcgcgttga actctcgggc aaacaggccg acctgtacga aaccatccga
     6121 ctcggcatgg aaaagaccgt gcgcgaggcg ctcagcacca aaggcctggc caagtcgcaa
     6181 atcaccatcc tcgatgccct gctgaaactg cgccaggtct gctgcgaccc gcatctgctc
     6241 aagcttgatg cggccaaaaa ggtgaaggta tcggccaagc tggagcagtt gatggccatg
     6301 ctgcccgaga tgctggccga aggacggcgc attttgctgt tctcgcagtt caccagcatg
     6361 ctcagcctga tcgaggacga actgatcaag cgcaacatca aatgggtcaa gctcaccggg
     6421 caaagtcaga agcgcgacga cctgatcacc cagttcacca gcggcgctgt gccgctgttt
     6481 ctgatcagcc tcaaagccgg tggtgtcggg ctgaacctgc cgcaagccga cacggtgatc
     6541 cattacgacc cctggtggaa cccggcggtc gaaaaccagg ccaccgaccg cgcccaccgc
     6601 atcggccaga cgcaaagtgt ctgggtggtc aagctggtgg cgcaaggcac gattgaagag
     6661 cgcattctgg cgctgcagga ccccaaggcg gcattggccg agagcatgta cagcggatca
     6721 gtcgggcgca agcagccgct gttttctgag agtgatctgg cagaactgct caagccactc
     6781 tctgtttgat agctggtggc gcttgttcca tctgggctag agggcgattt ggcttgtatg
     6841 tttggggtgc cagcatgatg tctggttgac tgatgcaatt tcaacggcgg gagttgactt
     6901 ggtttgggct gcaaaggacc cgacagcgac attcgacttt cagcgaccat cggcagggct
     6961 cgaccctgag cggaactagg acacagcgac ctcgcggccc aaaagcggcc gctcaacacg
     7021 cgtcacgatt cggagcaggg agtttcgata gaagccccac acgcgtcgat aactctgcgg
     7081 ttgacccctt gtagaaccac cactacaatt tttcggcaaa ttatggaggg gtgaaccatg
     7141 cgcaatgatg aatcgactgt ccggcgcctt gtcagtgcac gcacagggat agcggcagct
     7201 ctggcatttg ggctcgtcgg gtgcggaagc ctgccggacg ctaaggtcaa ctactaccag
     7261 accacgtcac agatcacggt caaggtgacg cgttcggtcc tttgtgacac gaagaatttc
     7321 cccgtcgtgg ccaacgtcgc agcgccgaac gtaacgcatt cagcggattt gacaaagccc
     7381 cgatccactg atctggcggg tctccgtggc tcctttacag atgcggatat caagttcgat
     7441 ctctacgaag acggccggct gaaggctgtg aatgcgaccc aaactggtca aggtgacgcc
     7501 gtcgtcaagg cggcaacgac tcttgcaacg acgattgcgg cctttagcac tacgtcgacc
     7561 ctgccttcct atccgactga atgtacgttc atcaaggatg tcggtggcgg caaaccactg
     7621 gctcttagct acgaaggcgt cttgaacttg tcgacgaagg attcccaaaa tattcctgcg
     7681 gacggtgcga gtgctgccta tgcgtcgcgc ctcagcgctt ttttgggtgg gatttgcgcc
     7741 aaagttggag agactgaggt acctgccaaa ttggtggagt acaccggctc tgatgccgtt
     7801 gtaacggcaa ctgcgcctgc aatggtctgg gtggaagtcg ggacggcacg accggggaac
     7861 ttgtgcgtcc ctgacttgtg gaaggggcgg ctgcctgttg cgcaactcgg caaggaatac
     7921 aagatccccc tgcctaatgc agttgttttt ggcaagcaga ctctgagcct tgccttcgcc
     7981 gaatcgggtg cagtgacgtc gatgcagtac acgactgcat caggggccgc aggtgctctg
     8041 ggtgccgtga atgcactggc gaccatcgcg cagcctgaat ccacgacggc aaagacgacc
     8101 gacctgaaga gccaagcgga cgagatcgtc caagagcagc gactgcttca gtgcaaggcc
     8161 gacccaaaga actgcaagtg acatgcagtt ttgattcaaa aggacggtgt actcatggcg
     8221 catcgaatct tgacgttgtt tgcactgttg ctcatcgcag cgcctgcttt ggcagtggac
     8281 ccggtcgtca tcacgctggt ctcggatggc gccgtaactg ctaagcccgg cagtggttcc
     8341 accgttgtcg agcgaggtaa ggccacggca actgcaaagg ccgacggcga cgaagacatc
     8401 gaaagctgcg aacggtatgg gcgtcgcagt tctgccaatg gtcgcgcagc agtcacaacg
     8461 gtttcggcag cgtcgcccac cagcctgagc tatcggttgg acgcagcggc atcggccagc
     8521 ggcgggcact atcggacagg cacttgcatt gctgatcggc gcattggctt cacgggacac
     8581 gatacggaag ccaaggtcga cgcggtggcc accgccgtcg tcaaaattca cttcaatggc
     8641 ggtcgaccga acgttccgta cttcgtaaaa atctcgcgct cacagagcgg gaccgcgcag
     8701 aacgaccagc tgacgggtcc cgatggcaca gtcattccgc tcaatgaccc gaagagcccg
     8761 cacccggtga tcttgagcaa gcctgggcag gactattttc tgaggacgac ggtcgcagcc
     8821 acggcgcaca atggaggcgg ctgctgcagt gaccaaatgt cggccatttc cgacatgtct
     8881 gtgttcattg agccagcgcc attgctcttt gccgccggac agtccccgtt tatcgcgaag
     8941 ggattccaaa ctacgggcta caagaacgtc gtggtcatca tgctggaagg cctgccccat
     9001 tgcactgcga ctatggtttc gccaactgta ctcgtcaccg cggctcactg cgtcaaagat
     9061 cacatgactc tcgacaagat gtcgacgggg aaggtgctcg cggtttttgg atctggctac
     9121 gcccagccac tgttcgcgcc ggtgcagatc gtgcgggcaa gctacccaga cactggtgag
     9181 caagcattcg atgcgtcgac tctgaaaaac gacattgccg tcgcctatct gaagatcgcg
     9241 atcactacgc ctggggtgaa ccccgcgcct ctgcacacgg gcacgccgag ctggcagcaa
     9301 atcaagacca acaagaccca cctgacattc gtcggatttg ggtatgacgt ggttggtggc
     9361 gagaagacgg gtgttggcgt caagcgcgag gcctcatggg caatcaccga ctacaccgat
     9421 agcatcgtct cgtttcagac tgacggcacc agcacgtgca acggggattc gggcgggccg
     9481 gcgttcttga atgtgaacca gacacttgtc ctggctgcaa ttacgtctgg cggtgatgac
     9541 aacgcctgca cttatggctg ggacactcga atcgacgcct atcttgattg gctcaagcca
     9601 aaaatcgcga gcgagtgagc ctgtaggctc aacccgaaac tacgccgtct gaaagacgca
     9661 tcagcggatc tgcgctgtgg tttccctacg agttcggggg gagctctcat caagattggg
     9721 gaatttgatt gcgtgaaagg ggagcttggg gcgttgagcg gcccgcgctc attgctcttg
     9781 tattgagagg ccgaccgata gcagacttcg gcgagtggcc gctcctggcc caaaccgggt
     9841 actcccgctg cattgcaaac actggattcg tgcccagcac cagcaacagg tggccgttcg
     9901 gcagccttgc atattgtctg cctcacggtc aaggcgccac acctttgttg ccagcccttg
     9961 cttgcgaccg cgtaatcgct aacccagagc gttcaattcg gtacctgaaa gtggtactat
    10021 aaaaagatga aacaacggca tcagcgaact gttgaattga ttttttcacg accgacgagc
    10081 gccaacgttc agtggcggga tgttgagggc cttttcgttg agctgggcgc agtgattgaa
    10141 gagcgctcag atagccgggt ttctgttttt ttgttcaatg aggtgcgggt ttttcaccgg
    10201 ccacatccgt cacccaacac ggacaaaggt gctgtggcat ctatccgcaa atggctggaa
    10261 caacacggag tcgaaccatg aacgtgatga ctgttgacga ctttcacgca acgattgcat
    10321 ttgaccctga tctggacatg ttcaggggtg aaattctcgg cctcaatggt ggtgcagatt
    10381 tttacggaaa gaatcctgac gaactgcgcg cggagttcaa gcgctcgctt gaggtatttc
    10441 ttgaagtctg caccgagaag gggattgagc caagacgtca tttttcggga aagttcaacc
    10501 tgcggatttc tcctgagttg cacgaaaaac tcgctattgc agcgcaggca aggggcaaga
    10561 gcatcaatgc cctggcccaa gaggctttgc aggatagctt tgcgtgaatt ggccagaagt
    10621 tggctgggca atccatcgga cggctgggcc gcccgctgag tttcaacgtt aaagtttcca
    10681 gtaagccgca ctggccaaat tgctcaagct tctattatat tgatagcttt cagcgcttat
    10741 tcagcaatgg cttgacgatg atttgacttg tattttcttt ggggattttc gaggtgcatc
    10801 tgactagcgg gcgcttgatg cctgtcagac aagcgtcacg ccccaatcat gcgatgcgtc
    10861 ttttgacgac tggcacgctg tggtccagcg tcaacaccgc ctcgcagcct agcgccttca
    10921 tttgcgcacc aatcaggtgg tcggcccaat cgccgccctc gcgttgccag actttgaacg
    10981 cggccacaac ggtgggcatg gactcaaagc gcaggcccac aaaacccagc aagatggtca
    11041 tggtttgctg acattgcgcc tgcgaatggt cgtacaagca ccccaacacc caaatcagtt
    11101 cacacagcac gatgtgtccc accaggcact cgcgccccgc tgccagttcg acatccagcg
    11161 cctcttgcat gcgctgcgcc tgcgccgggt cgtcatcggt caccagtcgc accagaacat
    11221 gggtgtcaag tgccagcatt tggataaccg ggtgttgatg agggcgtgac cggttttgca
    11281 gagcgcgttt tttctgttgc ggcatcttgc actgcctgat tcatttcctc gaccgtaccc
    11341 gcctgcttcg gctggggcaa gatgccgcgc agttttagca atatatggac ggtgcttttt
    11401 cctatcgtga agtcagccac ccaaagtccg acctgtttgc ggtcgatggg cgccagcaga
    11461 cccctacctg ccaatcacca aaattgccaa acgaccatcg gaaagtcgaa agctgccctt
    11521 cgatttcgtc ggaagcagac tttcggtttt ggtgcacgaa tctcggggcc ggtcattcag
    11581 tcgcgaaaat gatcttttca gactggcggt cggcactgca gcggtagcgg ccggtcgcta
    11641 cgaaaactgg gattgcgatg gtgcctccta tccggccttt ggcatctcgg gtccaatccc
    11701 cgcatttctc tggccgcaaa aatcgacccc ggtgattcga gggcgacgat cagttttcgc
    11761 cggtgtcggt cttgcggcgc gacagtgggg cataaactaa actgctatgc taaaagtagc
    11821 ggccacgctg gattgcgtgc cagacgccgt tgtcacaatg cagccataca ctatgaccac
    11881 cggtcggggg caaatgggct aaccttgact gttttcctgc cctgctctgg agctttttac
    11941 atgacaagat tgagacattt cccgctgact gcgctgctct caggtgtgct ggttttggcc
    12001 agtgtgatgc ctgccactgc gcaaacggct gccgccgcac ccaacaccac cgggccgagt
    12061 ggctcgggcg tgaacaatgc gttgctttac gctgccgcct ggaaacaaac cgccgccgaa
    12121 taccgtgcct tgtactacca gggcttcaat gtggcccgca tgcacgtcga aatggcgctg
    12181 agcaaacgca aagctggcga caaaccgctg gcggtcgtga ccgatatgga cgacaccgtg
    12241 ctgcacgcgc tgaactattg gggccatctg atcaagcaga acaaggatta ttttgacgat
    12301 ctgatctggg acaaatggat tccagaaaat aaggtcacgg catcgccagg gtcgctggat
    12361 ttcctgaagt tttgcgcgga aaacggcgtc gaggtgtttt atgtgactag ccgcgaccag
    12421 ggcgagaaaa cctacgaata tgcaatgggc cacttgaagg tgctgggttt tccctatgcc
    12481 gacgaaaaac atctgaccgt gttgcgggac acctccaaca aggaaaagcg ccaggacgaa
    12541 atcatgaaag acttcagtgt ggtggtattt ctgggtgaca acctgaacga ctttcgccgc
    12601 aagtactaca tcaagagcaa tgtggatgag cggctcaaga tgatggaaac cgaccgcgag
    12661 aagtatggtc gcaattacat tgtgttcccc aacccgaccg acgggcattg gctggcggca
    12721 atttttggcg aatcagaacc acccccgacc gatgccaatc gcgaaatcat gaagaaggcg
    12781 gcgaccaagt cggcttggtg aacaaaccgg ggcaccttgc tgtttgattt gcctgtttac
    12841 gcacgggcaa aacgcgacaa gtcgctgcag cctctggccg cagggggcaa gttcatgcag
    12901 gacatgcgtg agcaccaaag gcctggccaa gtcgcaaatc accatccttg atgccctgct
    12961 gaaactgcgc caggtctgct gcgacccgca tttgctcaag cttgatgcag ccaaaaaagt
    13021 gaaggtatcg gccaagctgg agcagttgat ggccatgctg cccgagatgc tggccgaagg
    13081 acggcgcatt ttgctgttct cgcagttcac cagcatgctc agcctgatcg aggacgaact
    13141 gatcaagcgc aacatcaaat gggtcaagct caccgggcaa agtcagaagc gcgacgacct
    13201 gatcacccag ttcaccagcg gcgctgtgcc gctgtttctg atcagcctca aagccggtgg
    13261 tgtcgggctg aacctgccgc aagccgacac ggtgatccat tacgacccct ggtggaaccc
    13321 gg
//
LOCUS       DAOSLL010000046        13037 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4847_length_13037_cov_4.325605, whole genome shotgun sequence.
ACCESSION   DAOSLL010000046 DAOSLL010000000
VERSION     DAOSLL010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 13037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13037
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4847_length_13037_cov_4.325605"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="PLB25_03325"
     CDS             <1..191
                     /locus_tag="PLB25_03325"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOE40652.1"
                     /translation="VTEAQNQAGQEFSEDRCKDRLGQLHSQPLQHVLDDMRRQVATFT
                     GTEVLEDDFTMLALRRKV"
     gene            628..2604
                     /locus_tag="PLB25_03330"
     CDS             628..2604
                     /locus_tag="PLB25_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HOE40653.1"
                     /translation="MTRHFPDFTASSDHALAALGKDASNASLPRRQVLKSALGGVATL
                     GLSSLGLSACGGSDAAPVTISSVEFIGMSAPGTDALRAASYTSASVKITYSDATTKSQ
                     ALGYNTLFKTGDTLKNGNASVIAGAYYDINGNPIIDTSGATAQQFFSDCPDGNSLISL
                     ANPTVAGVTGNTLFLVTQYEYATANNAASGTRPTPTASQMYGTLPSPITVTTLDQDKT
                     TGALKVVKHFNVDTKPANGLWITCASSLSPWNTHLSSEEYEPDAVNIATNTMFQAFSK
                     NLFGSTTTANPYHYGHLPEVTVKPDGTATIVKHYCLGRIARELVEVCPDNKTVLMGDD
                     GDNTGLFMFIANAEKDLSAGTLYVAKWTQTSATSGGAASLSWIKLGSATSAEIKALAD
                     THSASDIVTVSKTDPLDATYTKIAFNGATEWVKFAAGKEKAAAFLETHRYAATLGGSL
                     EFTKMEGVTLNRKDKVAYIAMSRIEKSMADTSGDIQVGKITAGATYELKLSAGQKDKA
                     GATIGSDWVPTAMAAIPALVGEDQAADAVGNTANVDKVATPDNLKFSEKLRTLFIGED
                     SATHVNNFVWAYNVDTKKLSRLQSVPAGGEATGLQIVDNLNGFSYIMSNFQHAGDWGS
                     IHSIISAGVDPLINANWNNKKSSAVGYISGLPVL"
     gene            complement(3053..5017)
                     /locus_tag="PLB25_03335"
     CDS             complement(3053..5017)
                     /locus_tag="PLB25_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HOE40654.1"
                     /translation="MSHSNSSSRRSALKMMAAAPMLPLGAFSVGSLLSGCASTTATGT
                     GSAAASGAFASAAFTAMPAPSLANPAAMATTTVASGLQLSMKDGSTRAYKLAYQPFFI
                     TGDRVPDGKGGTVLAGGYVDIVSRPIIDKSVPGKERQFFSDCPDGTSLLKLEHPKVAG
                     VKGKAVFAVVQFEYANADQGGADTYGTLPSPIAVLTLDQDQTTGALTLVKYHSVDTSE
                     AQGLWITCGSSLSPWNTHLSSEEYEPDAPFVTENKQFKAFSKNLFGDENVANPYHYGH
                     LPEVTVHPDGSGTIVKHYCLGRISHELIQVMPDNRTALMGDDYTNSGLFVFVADKEKD
                     LSAGTLYVAKLGSGFSINPADVGTRLSWIKLGHATSKEIEKLANTLKPADIMTVLKAD
                     PNDASFTKVFYNGAANWVKVKPGMEKAAAFLETHRYAYLMGGSMGFSKMEGTTVNIKD
                     KVAYSALQNIQDSMVKTGKGWNAASGIALDKALVAGGVMALPLAGGQVDQAGAAIKSE
                     WLPVAMRALLMGEDIAPDALGNLANPDKVSNPDNLKFSEKLRTLFIGEDSGTHVNNFV
                     WAYNVDTKKLSRLMSMPSGAEATGLGVVDDLNGWMYITSNFQHPGDWNAKLHGKVQAT
                     LDPLVRANYKDRFGAAVGYLTAQTSGIKLG"
     gene            complement(5359..9699)
                     /locus_tag="PLB25_03340"
     CDS             complement(5359..9699)
                     /locus_tag="PLB25_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE40655.1"
                     /translation="MPTQNHPEVLLQLLSVAPHTVSSVAGDVESMLGYSASKLLQGEV
                     SILALIHPDDQDVLDAMLHIQPQSAAQPQTHVCNLRLRQASGRIRCLVAQYAHELAAT
                     GVVLNLRLRDAKSLFDPVNALAADPGFRSMMESTNDFIFFKDRHHVFTAASQTLVSVT
                     QPSTHWTDLIGQTDYDVFPEEYADIYYRLEKQVFAGKTVAHEVQEYQTSDGVKGWVDN
                     RKYPIRTAQGEITGLFGIARVMTEQVRAEQALRLERDSTRNILATVEAMIVALDTGGR
                     ITLVNRKGCQILGYREAELIGQDWFATCLPRTIDVEQVRDIFKMSLAGNLAGAEYFEN
                     PVLTRSGEERLIAWHNSTIRDKDGHTIGGLSAGEDITERKQKEHELQQREQYQRALLD
                     NFPFAVWLKDTESRFLTVNQGFVQLFGQRSAIELVGKTDFDIAPTELAKGYQADDRAV
                     LGTGKKKNVEEEIVDADGVRKWFETYKAPVFNDIGSVVGSVGFARDITERRAAAQAMA
                     ELSKALAVSRDLLQKVIDTAPIRVFWKDRDCRYLGCNPAFARDAGKSAPADLIGLDDY
                     AMCWVEQADLYRADDRQVMESGQPRLDFEEPQTSPDGKPLWLRTSKVPLRDAAGVVIG
                     VLGLYDDITERRQAEIALRHEKQFSEDSLNALPGVFYMFDTTGRFVRWNHQFNKVTGY
                     TDAELASMRGTDFFVGEDQRLVAEAMQRVFAQGQAEVEADFQTRDGGKLPYHFIGHRS
                     TIGDQIYLLGVGLDISEQRRTRQALEIQRTHLQTLVNTIPDLIWLKAGDGTYLACNPE
                     FERFFGAAENDIVGKTDHDFVPKALADAFRKNDCAAIANGEATRNEEWITYASDGHRV
                     LLETTKIPMRTPDGRLVGVLGIGHDITEREAHQKQLEHIAHFDTLTSLPNRVLLADRL
                     QQALAQAQRHGKVLAVAYIDLDGFKAINDKFGHDVGDRMLTALAGHMKKSLREGDTLA
                     RLGGDEFVAILLDLSDVESSAPLLSRLIAAAAEVVYEDGNALRVSASLGVTFYPQVEA
                     IDSDQLLRQADQAMYQAKQAGKNRYHVFDTEHDRTVRGHHESLERIRQALDEREFVLY
                     YQPKVNLRTGVVIGAEALIRWQHPQQGLLPPASFLPLIADHPLAIEMGEWVLATAMTQ
                     VEDWKRVGIALPVSVNIDAIHLEQADFVDRLRQQLSRHPGVVAGDLELEVLETSALQD
                     IAHVSGVILACNEMGVGFALDDFGTGYSSLTYLKRLPAGVLKIDQSFVRDMLEDPDDL
                     AILDGVLGLASAFRRQAIAEGVETLAHAEVLLQLGCPLVQGYAIARPMPASSMPSWLA
                     TWRPDAAWFSRAPIRREDMPILYAWVEHRAWIAHVLGYLQGERSDAPPLDCKACRVGQ
                     WLGHARGHHANHSAAIAAVEPLHVEIHARATEMIRFKQLGQTDVLPTQMEGFYRLRDR
                     LLAQLFEMLR"
     gene            complement(10002..10598)
                     /locus_tag="PLB25_03345"
     CDS             complement(10002..10598)
                     /locus_tag="PLB25_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOE40656.1"
                     /translation="MRTWGWRFKWRARFTAILDANVLYPQLIRDTLLSLAVERLYHAR
                     WSATIHAEWTRNLAKDRPELADKLPLVVKRMNASVPDCLVTNYEKLANSIELPDPDDR
                     HVVAAAIVGHADAIVTFNTKDFPMAVLAPYGIEVQHPDEFLMNQLQLQEIPALRAIKK
                     MRARWTNPARPAQELITAFEKRGLPMTADLLRQAVTLI"
     gene            complement(10573..11049)
                     /locus_tag="PLB25_03350"
     CDS             complement(10573..11049)
                     /locus_tag="PLB25_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE40657.1"
                     /translation="MTAQEVLDPANAAEAEMAAVAHQCLVTALDHSNADHINIILDVG
                     TDGDEKHETPVLKLPPRALRFFADVLRQMAKQEPMLLVPQKHELTTQEAANFLNVSRP
                     FVIKEIEANRLKCRKVNRHRRIEFEELRRYQAAQQQQSEQALQDLTQLSEDLGLAF"
     gene            11250..11519
                     /locus_tag="PLB25_03355"
     CDS             11250..11519
                     /locus_tag="PLB25_03355"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOE40658.1"
                     /translation="MTDLTIEALVARLQAAQVKTVVDVRELPLSRKKGFSKSAFYAAL
                     SAHRIAYLHAPALGCPKPIHNQYKADGNWQTYTREFLKCIPIQGF"
     gene            11852..12343
                     /locus_tag="PLB25_03360"
     CDS             11852..12343
                     /locus_tag="PLB25_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40659.1"
                     /translation="MVVLRVPAAMAQPVSFHEVEYIRIHSAKVKLASHADKEARLWAR
                     LNTRADWSAELVSTATVADLDASALAVGRLRFAEKHPHLAGEVAGWDVPTLLSRLIRV
                     CQLGLAGGQAITSDHRNSCCAKPLREGGRFSIVVAWLHQNPPADKGYARPLTPRMKEK
                     VSR"
     gene            complement(12405..>13037)
                     /locus_tag="PLB25_03365"
     CDS             complement(12405..>13037)
                     /locus_tag="PLB25_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2403 family nucleotidyltransferase fold
                     protein"
                     /protein_id="HOE40660.1"
                     /translation="LEQHQRLNRALRMGRVDPLVVALLGRLVSTQLSPHFRVVGTQAL
                     YAYEATAGVRLDSETLATRKRILFSTQLVKVDSSMLGVLKKVDNTFRIRKSQKYTAVN
                     KDGFEVDIIRRERTGDDPHPIKLSDDDDDFWVVQARRANVLLDSPGFSAVIVATNGTM
                     ARMNTVHPATFVAFKRWMAGQPDREALKRRRDVLQADAVQELLDKHLPQI"
BASE COUNT         2675 a         3815 c         3807 g         2740 t
ORIGIN      
        1 gtgtgaccga agcgcagaac caggcagggc aggagttttc cgaagaccgc tgcaaggacc
       61 ggctgggtca gctccattca cagcctttgc agcacgtgct tgatgacatg cgccgccagg
      121 ttgccacttt taccggaacg gaggttctgg aagacgactt caccatgctg gcgctgcggc
      181 gcaaggtgta agcaaaaatt gcgcagtcgg acccgcccga gtgggccact ggcagcgcgt
      241 ccgggtaggg cgaaagctgc ggctccagac gtgtttgcga aagtctggac ggccgcagca
      301 ggccagcttg gtgacaaggc tgcgcttgcc ggggcgtgtg aaagggcgtt gggtaggcgc
      361 gacgacgacg ctcgctactg cccgccagga agagcaacgc cagttgcggt gccagcggcc
      421 ctttttgcac cggccttttg acccattgcg gagctatgcg cctgcaaatc cggcacccct
      481 cgctggggtc aatgctcgct gttcgatcca taggtgccac aggcacctgg ctggcctggc
      541 tcataaggtc gctttccatg ggggcagccg gtcacacggc tgtcacacgc tcaaactaga
      601 ttcaggtctt ttcaaccgga gccttacatg acccgacatt tccctgattt cactgccagc
      661 tctgaccatg ccttagccgc cctgggcaaa gatgccagca acgccagctt gccccgtcgg
      721 caagtactca aaagcgcatt gggtggtgtt gccaccttgg gcctgagcag ccttggtctg
      781 agcgcttgtg gtggcagcga tgccgccccg gtgaccatca gctcggtcga atttatcggc
      841 atgtcggccc ctggcactga cgcacttcgc gctgccagct ataccagcgc cagcgtcaag
      901 atcacctact ctgatgccac gacgaaatcc caggcgcttg gctacaacac tcttttcaaa
      961 accggcgaca ccctcaaaaa tggcaatgcc agcgtgattg ctggtgccta ttacgacatc
     1021 aatggcaacc ccatcatcga cacatcgggt gccacagcac aacagttttt ctccgactgc
     1081 ccggatggca actcactgat ttcgctggcc aacccgacgg ttgcaggtgt tacaggcaac
     1141 acgttgttcc tggtaacgca gtacgagtac gcaaccgcca acaacgctgc cagcggcaca
     1201 cgcccgacac caaccgcttc gcaaatgtat ggcacgctgc cgtcaccgat caccgtgacc
     1261 acgctggacc aggacaagac cactggcgcg ctcaaggtcg tcaaacactt taatgttgac
     1321 accaagccgg ccaacggact gtggatcacc tgtgcatcga gcctgtcacc ctggaacacc
     1381 cacctgtcca gtgaagaata cgaaccggat gccgtcaaca ttgccaccaa cacgatgttt
     1441 caggcgttca gcaaaaatct gtttggcagc accaccacgg ccaatcccta tcactacggc
     1501 catctgcccg aggtcacagt caaacctgac ggcacggcga ccatcgtcaa gcactattgc
     1561 ctgggccgca ttgcgcgtga actggttgaa gtgtgtcctg acaacaagac cgtgctgatg
     1621 ggcgacgacg gcgataacac cggactgttc atgttcatcg ccaacgcgga aaaagacctg
     1681 agcgcaggca cgctgtacgt cgcgaaatgg acgcagactt cagccacttc gggcggcgct
     1741 gcgtcgctct catggatcaa gctgggcagt gccaccagcg cggaaatcaa ggcactggcc
     1801 gacacgcaca gcgccagcga catcgtcacc gtctcgaaga ccgatccgct cgatgccact
     1861 tacaccaaga tcgcctttaa cggtgccacc gagtgggtga aattcgccgc tggcaaggaa
     1921 aaagcggcgg ctttcctgga aacccaccgc tacgccgcaa ccctgggcgg ctcgctggag
     1981 ttcaccaaga tggaaggtgt gacgctcaac cgcaaagaca aggtggccta catcgccatg
     2041 tcgcgcatcg aaaaaagcat ggcagataca tctggcgata tccaggttgg caagatcacc
     2101 gccggggcaa cctatgaact gaagttatct gcaggccaga aggacaaggc gggtgcgacc
     2161 atcggaagcg attgggtgcc cactgccatg gccgccattc cagcgctggt cggcgaagac
     2221 caggccgcag atgcggttgg gaacaccgcc aatgttgaca aggttgccac acccgacaac
     2281 ctcaagttct cggaaaaact gcgcaccttg ttcattggcg aagactccgc cacccacgtc
     2341 aataactttg tctgggcgta caacgtggac accaagaagc tctcgcggct ccaatcggtg
     2401 cctgcgggtg gtgaggccac cggtttgcaa atcgtggaca acctcaacgg cttttcctac
     2461 atcatgagca atttccagca tgcaggtgat tggggatcaa tccattcgat catcagtgcc
     2521 ggtgtcgatc cgctgatcaa tgccaactgg aacaacaaga agagttccgc agtcggctac
     2581 atcagcgggc tacccgtact ctgattcgcc ggcaatccag gtcccaggcc ggacgagctg
     2641 gaatcgaacg gccttcgtcg caagggcgcg actcgcacaa gacatctgaa gcattttgag
     2701 tttgtgggag ttgcgccctc gcgacgaatg acgctatgac caagcagtgg gccaaacccg
     2761 ttgcctgtac cggcatcctg gaatagcaca gccacggtgc tatcctagtc atagcttgct
     2821 gcgcagacag ggcaagggct acatgtaaaa atgtgcttga taccgcgtgc tattggtgcc
     2881 gtggtctcgg tcgcttctat tgcccaagaa ccctgaccgg cctggtcaat atcgtcaaca
     2941 aaagcaaggg ttgtcgcgaa ccgggggtga ggatcagacg tgggcagaag gcacagttcg
     3001 tgtgaactgt gccttccagt tttggctata ttgacacgcc cccagcgggc aactacccaa
     3061 gcttgatccc gctggtttgc gctgtcaggt agcccaccgc agcaccaaag cggtctttgt
     3121 agttggctcg caccagtggg tcgagcgtgg cttgcacctt gccatgcagt ttggcgttcc
     3181 agtcgcccgg atgctggaaa ttgctggtga tgtacatcca gccattcagg tcgtccacca
     3241 cacccagacc agtagcctcg gcaccggacg gcatcgacat caggcggctg agttttttgg
     3301 tatcaacgtt gtaggcccag acaaagttgt tgacatgggt gccgctgtct tcgccaatga
     3361 acagcgtgcg cagcttttcc gagaacttca ggttgtccgg gttggacacc ttgtcggggt
     3421 tggccaggtt gcccagcgca tcgggggcaa tgtcttcacc catgagcaag gcgcgcatgg
     3481 ccacaggcaa ccattcgctc ttgatggcgg caccggcctg atcgacctgc ccgcccgcca
     3541 ggggcaaagc catcacacca cccgccacca gtgccttgtc cagtgcaatg ccgctggcgg
     3601 cgttccagcc cttgccggtc ttgaccatcg agtcctgaat gttctgcagt gccgaatagg
     3661 ccaccttgtc tttgatgttg acggtggtgc cttccatttt ggaaaaaccc atgctgccac
     3721 ccatcaggta ggcgtagcgg tgagtttcaa ggaaagccgc cgccttttcc atgccgggct
     3781 tgaccttgac ccagttggcg gcgccgttgt agaacacctt ggtaaagctg gcatcgttgg
     3841 ggtcggcttt cagcaccgtc atgatgtcag caggcttgag cgtgttggcc agcttttcga
     3901 tctccttgct ggtggcatgg ccgagcttga tccaggacag cctggtgccg acatctgccg
     3961 ggttgatcga gaaaccgctg cccaatttgg cgacatacaa ggttcctgcg gacaagtctt
     4021 tttccttgtc ggcgacaaac acgaacaggc cgctgttggt gtaatcgtcg cccatcaggg
     4081 cagtacggtt gtctggcatg acctgaatca gttcgtgcga aatgcggccc aggcaatagt
     4141 gcttgacgat ggtgccgctg ccatcggggt gcaccgtgac ttcgggcaga tggccgtagt
     4201 ggtaagggtt ggcgacgttc tcgtcaccaa acaggttttt gctgaaggcc ttgaactgct
     4261 tgttctcagt gacaaacggc gcatcaggct cgtattcttc actggacaaa tgggtgttcc
     4321 agggcgacag gctggagccg caggtaatcc aaaggccctg ggcttcggaa gtatcgacgc
     4381 tgtggtactt gaccagcgtc agcgcgccgg tggtctgatc ttgatcgagc gtcaacaccg
     4441 caatcggtga aggcagcgtg ccataggtgt cagcaccgcc ctggtcagcg ttggcatatt
     4501 caaactgcac cacggcaaac accgccttgc ccttgacgcc tgccaccttg gggtgctcaa
     4561 gcttcaacag cgaggtgcca tccgggcaat ccgagaaaaa ctggcgctct ttgccgggca
     4621 ccgatttgtc gatgatcggg cgactcacga tgtccacata accacccgcc agcacggtgc
     4681 cacccttgcc gtcaggaacc cggtcaccag tgatgaaaaa tggctgatag gccagcttgt
     4741 aggcacgggt actgccgtct ttcatgctca actgcaagcc cgatgccacc gtggtggtcg
     4801 ccatggcagc ggggttggcc aggctgggtg caggcatggc ggtaaaggcg gcagaggcaa
     4861 atgcgcctga cgcggcggct gaaccggtgc ctgttgcggt ggtcgaggca caacctgaca
     4921 gcagcgagcc aaccgagaac gcccccaacg gcagcatggg cgcagcagcc atcattttca
     4981 gggcggaacg gcgagaagat gaatttgaat gggacatggt taaggttacc ggaggttgat
     5041 ggatgagcaa cgcagcatgg taaagacttg atgtgacaga ttgatgaaag ttttgagtca
     5101 cgcctgcgtg cgctgccgca gataacagta tggacatcac cccgaccctg acacaccagt
     5161 ttgtcggcga tcgttgatcg ccagctcaaa gatctgatac cggcacaaac ctggacattc
     5221 atgacagcct gcctgcacct gcaccgccgg ccagacatcc ggctgtcgat cagctatgtt
     5281 atatatagct ggcggcgcaa taccagtgag ggctagaagg caaaaatgct ctttgggttg
     5341 cggtcaattg cgggttgact agcgcaacat ctcgaatagc tgggcgagca aacggtcgcg
     5401 caagcggtaa aacccttcca tttgcgtcgg cagcacatcc gtctggccaa gttgtttgaa
     5461 gcggatcatc tctgtcgccc gggcatggat ttcgacatgc aaaggttcaa ccgccgcgat
     5521 cgctgctgaa tggttggcat ggtgcccgcg ggcgtgcccg agccattgcc caacacgaca
     5581 ggccttgcaa tccaggggcg gcgcgtcgct gcgttcgcct tgcaaatagc ccaaaacatg
     5641 cgcgatccag gctcggtgtt ccacccaggc gtacaatatg ggcatgtcct ctcggcggat
     5701 cggcgcacgg ctaaaccagg ctgcatccgg gcgccaagtt gccagccagc ttggcatgga
     5761 ggatgccggc atggggcgcg cgatggcata cccctgcacc agtgggcagc cgagttgcag
     5821 cagaacctcg gcgtgggcca gggtttccac cccttcggcg attgcctgac ggcgaaacgc
     5881 gctggccaag ccgagcacac catccagaat cgccagatcg tcaggatctt ccagcatgtc
     5941 gcgcacaaag ctctggtcaa ttttcaacac gccagcgggc aagcgtttca gataggtcag
     6001 cgaagaatag ccagtgccaa agtcgtccag tgcaaagcca acccccattt cattgcatgc
     6061 caggatcacg cccgagacat gggcgatgtc ttgcagcgcg ctggtttcca gcacttcgag
     6121 ttccaggtcg ccggccacga ccccgggatg cctggaaagt tgctggcgca agcgatcaac
     6181 aaaatcagct tgttccaaat ggatcgcatc gatattgaca ctgaccggca gtgcaatgcc
     6241 gactcgcttc cagtcctcga cctgcgtcat cgccgtagcc agcacccatt cacccatttc
     6301 aatcgccagc ggatggtcgg caatcagcgg caaaaaagag gccggtggca gcaagccctg
     6361 ctgcgggtgc tgccagcgga tcaaggcctc ggcaccgata acgacacccg tgcgcaggtt
     6421 caccttgggc tggtaataaa ggacaaattc ccgctcgtcc agcgcctgcc tgatgcgctc
     6481 caggctttca tgatgaccgc gcacagtgcg atcgtgctcg gtatcgaaga cgtgatagcg
     6541 atttttgcca gcctgcttgg cctggtacat cgcctgatcg gcctgacgca gcaactggtc
     6601 ggaatcgatg gcctccacct gcgggtaaaa agtaaccccc aggctggcgg aaacccgcaa
     6661 ggcattgcca tcttcgtaaa ccacttccgc cgccgctgcg atcaggcgcg aaagcaacgg
     6721 tgcagaggat tcgacgtccg acaaatcaag cagaattgca acgaactcat cgccacccag
     6781 acgcgccagc gtatcgcctt cgcgcaacga ttttttcatg tggccggcga gggcggtgag
     6841 catgcggtcc cccacatcat gaccaaactt gtcattgatc gccttgaatc catccagatc
     6901 aatataggcg accgccagca ccttgccgtg gcgctgcgct tgcgccaggg cctgctgaag
     6961 gcgatctgca agaaggacac gattgggtag actggtcagg gtatcgaaat gggcaatgtg
     7021 ttcgagttgc ttctggtgtg cctcgcgctc agtgatgtca tggccgatcc cgagaacacc
     7081 caccaatcgg ccgtctggcg tgcgcatcgg tatcttggtg gtctccagca gaacgcggtg
     7141 gccgtcgctg gcgtaggtga tccattcctc gttgcgggtt gcctcgccgt tcgcaatggc
     7201 ggcgcaatca ttcttgcgaa atgcatccgc caacgccttg ggcacgaagt cgtggtcggt
     7261 tttgccgacg atgtcgtttt ctgccgcgcc aaagaaccgc tcaaactccg ggttgcaggc
     7321 gaggtaggtg ccgtcgcccg ccttgagcca gatcagatcg gggatggtgt tcaccagggt
     7381 ttgcaggtgc gtgcgctgga tctccagcgc ctgccgtgtg cggcgctgct cgctgatatc
     7441 gaggccaaca cccagcaggt agatttgatc gccaatggtg ctgcggtgcc ctatgaagtg
     7501 gtagggcagt ttgccgccat ccctggtctg gaaatccgcc tcaacctccg cctgcccctg
     7561 ggcgaatacc cgctgcatgg cttcggccac cagccgctgg tcttccccga caaaaaaatc
     7621 ggtgccccgc atgctggcca gttcggcatc ggtgtaaccg gttaccttgt tgaactgatg
     7681 attccagcgc acaaagcggc cagtcgtgtc gaacatgtag aacacgccgg gaagggcatt
     7741 cagggaatct tcagaaaact gcttctcgtg gcgcagcgcg atttcggcct gtctgcgttc
     7801 ggtgatgtcg tcgtacagcc ccagcacgcc aatcacgacg ccagccgcgt cgcgcagcgg
     7861 caccttggag gtgcgaagcc agagtggctt gccgtcgggg ctggtctggg gttcttcgaa
     7921 gtcgagtcgc ggctggcccg attccatcac ttgccggtca tcggcacggt agaggtcggc
     7981 ctgctcgacc cagcacatgg cgtagtcgtc caggccgatc aggtcggccg gtgcgctctt
     8041 gccggcatcc cgggcgaagg ccggattaca gcccagatag cggcagtccc ggtctttcca
     8101 gaagacgcga atcggtgcgg tatcaatgac tttctgcagc aagtcgcgcg atacggcgag
     8161 ggcttttgac aattccgcca tggcctgcgc ggcagcacgg cgctcggtaa tgtcgcgggc
     8221 aaagccgacg ctgcccacca cgctgccaat atcgttgaaa accggcgctt tgtaggtctc
     8281 aaaccacttt cgcaccccgt cggcgtcgac gatctcctcc tcgacgttct tctttttccc
     8341 ggtgccaagg acggcccgat catcggcctg atagcctttt gccaactccg tgggcgcaat
     8401 gtcgaagtca gtcttgccga ccagttcaat ggcgctgcgt tggccgaaca gttgcacgaa
     8461 cccctggttg acggtgagaa aacggctctc ggtatccttg agccaaacag cgaaggggaa
     8521 gttgtcaagc aaggcccgct ggtactgctc gcgctgctgc agttcgtgct ctttttgctt
     8581 gcgctcggtg atgtcttcgc cagcagaaag gccgcctatg gtgtggccgt ctttgtcgcg
     8641 gatggtgctg ttgtgccagg caatcagccg ctcctcgccg ctgcgggtaa gcaccgggtt
     8701 ctcaaagtac tctgccccgg cgaggttacc cgccagggac attttgaata tgtcgcgcac
     8761 ctgttcaaca tcgatggtgc gcggcaggca agtggcaaac caatcctggc cgatcaattc
     8821 ggcttcgcgg tagcccaata tctggcaacc cttgcggtta accagggtga tccgcccccc
     8881 ggtgtcgagc gcgacgatca tcgcctccac cgtcgccaga atgttgcggg tgctgtcgcg
     8941 ctccagccgc aacgcctgtt cggcccggac ctgttcggtc atcacgcggg cgatgccaaa
     9001 caggccggtg atctcgccct gggcagtgcg gatcgggtac ttgcggttgt caacccaccc
     9061 tttcacgcca tcactggtct ggtactcctg aacctcatgc gccaccgttt tacccgcaaa
     9121 tacctgcttt tccaggcggt agtagatgtc ggcatattcc tcggggaaca cgtcgtaatc
     9181 ggtctggccg atcaggtcgg tccagtgcgt cgagggctgg gtcacgctga ccagggtttg
     9241 gctggcagcg gtaaagacgt gatgtcgatc cttgaaaaag atgaaatcgt tggtgctttc
     9301 catcatcgag cgaaaacccg ggtcggcagc tagtgcattc accggatcaa acaggctttt
     9361 ggcatcgcgc agtcgcaggt tgagaaccac gccagtcgct gccaattcgt gcgcatactg
     9421 cgcaaccaga caacggatgc ggccacttgc ctgacgcagg cgcaggttgc agacgtgggt
     9481 ttgtggctgg gcggcagact gcggctgaat gtgtaacatc gcgtccagca catcctgatc
     9541 atcagggtgg atcagcgcca gtatcgatac ctctccttgc agaagcttgc tggcgctgta
     9601 cccgagcatc gattcgacat cgcccgcaac cgaagaaaca gtgtgcggcg caaccgacag
     9661 aagctgcaat aaaacttccg gatgattctg ggtaggcatg agacttgttt tttcgccaat
     9721 gctcggcggg tttgagttca ttctattgct cacagcccgg ttccattcgc atcgttttac
     9781 cttcgcgata cgcaattggc ctgctttcaa cgactgcgac cgttgctgct caagtttgac
     9841 aagcgcttgg tgcgcaacaa gctcattgac ctgtccatcc tgcgcggtgt catcaggctg
     9901 acccagccaa tggcgggcaa aacggtgaat cggttgattg gttgctcttt aacttatagc
     9961 tgcttgcgca cattccacga gggctacaga catgttttgc atcaaatcag cgtaacagcc
    10021 tgacgcagca aatccgccgt catcggcagg cctcgtttct caaacgccgt gatgagctct
    10081 tgcgctggcc gtgcagggtt tgtccagcgg gcacgcatct ttttgatggc tctgagcgct
    10141 ggtatttcct gcaactgcaa ttggttcatc aagaactcat cggggtgctg aacttcaatg
    10201 ccgtagggcg ccaacacagc catcggaaag tccttggtgt tgaaggtgac gatagcgtct
    10261 gcatgaccca caatggcggc ggccacgacg tggcggtcgt cggggtcagg caactcaatg
    10321 ctgttggcca gtttttcata atttgtcacc aagcagtcgg gcacgctggc gttcatgcgt
    10381 ttgaccacca gtggtagctt gtcggcaagc tctggccggt ctttggccag gttgcgcgtc
    10441 cattccgcat gaatcgtggc gctccagcgc gcgtggtaca ggcgctcaac ggccaggctc
    10501 agcagggtgt cgcggatcaa ttgcgggtac agaacgttgg catccagaat ggcggtgaac
    10561 cgcgcccgcc acttaaaacg ccagccccag gtcctcagac aattgagtca ggtcttgcag
    10621 ggcctgctca gattgctgtt gctgcgcggc ttggtagcgg cgcagttctt caaattcgat
    10681 gcggcgatgg cggttgacct tgcggcattt gagacggttg gcctcgattt ccttgatgac
    10741 aaaggggcgc gacacgttca aaaaattggc tgcttcttgc gtggtgagtt cgtgcttttg
    10801 cggcaccagc agcatgggct cttgtttggc catttgccgc agcacgtcgg caaaaaagcg
    10861 caaggcgcgg ggcggcagtt tcagcacggg tgtttcgtgt ttctcgtcgc cgtctgtgcc
    10921 cacatccaga atgatgttga tgtgatccgc gttggaatgg tccagcgccg tcaccaggca
    10981 ttgatgtgca acggctgcca tttcggcttc agcagcattg gcggggtcta gtacttcttg
    11041 ggctgtcatg gggtttcctt cgcgtagcgg ccttggggtt tggccattcg caataagtcg
    11101 gatcgtagca ttattcgaag caaacgaatt agtcgcatta agtgaatgct cttgctgggt
    11161 atttgatgaa ttgagaaaat cgtcctgcca caccgccaaa agtccatgca aatcaacaac
    11221 ttgagaatca acgggtactt gctggatatt tgacggacct gactattgag gcgttagtcg
    11281 cccgcctgca agccgcgcag gtgaaaaccg ttgtggatgt ccgtgagcta cccctgtcgc
    11341 gcaagaaagg attttcaaaa tcggctttct acgccgcctt gtcagcccat cgcatcgcct
    11401 atctgcacgc ccccgccctg ggttgcccca agcccatcca caaccaatac aaggcggatg
    11461 gcaactggca aacctacacg cgcgaatttt tgaagtgcat ccccatacaa gggttttgaa
    11521 tcgcatgaat tccggtgatc tgaccttatt gcttgaccgc ctgcaggccc caagcgggcg
    11581 aacacgagtg gctggagttc aaacacaacg gcgatccgca caccatggga gagtacgcct
    11641 ctgccctggc caatgcggca gcgttggacg gtgagccttt ttggttatat ggtctggggc
    11701 attgaaaacg acacccgcca agtaatggga acccgcttta aacctggttc agccaaggtg
    11761 gggggcgcaa ataatggatt tatggttgca ccaatacttg cgtcccaagc cggatttccg
    11821 ttttgatgcg tttgactacc acggccagcc cgtggtggtg ctgcgcgtac ctgcggcaat
    11881 ggcgcagccg gtgagttttc atgaggtgga gtacatccgc atccacagtg ccaaggtcaa
    11941 actcgccagt catgccgaca aagaggctcg gctatgggca aggctgaata cgcgggccga
    12001 ttggtcagcc gagctggtga gcaccgccac cgtggccgat ctggatgcat ccgcactggc
    12061 cgtggggcgg ctgcgttttg cagagaagca tccgcatctg gcgggtgagg tggcaggctg
    12121 ggacgtgccc acgctgttgt ccaggctcat tcgtgtttgc cagcttgggc tagcaggcgg
    12181 tcaagcaata acatcagatc accggaattc atgttgtgca aaacctttgc gtgaaggtgg
    12241 gaggttttct attgttgtgg cgtggttgca tcagaatccg cctgctgata agggctacgc
    12301 acgccctctt acgccgcgta tgaaggaaaa ggtaagtcga taggcactac tgctttagta
    12361 gctgctttcg tacattcctc gcgggctaga ggtcaatttg acacttaaat ctgcggcaaa
    12421 tgcttgtcca gcagttcttg cactgcatcc gcctgcaaca catcccggcg gcgtttgagt
    12481 gcttcccggt caggctgccc ggccatccag cgcttgaacg cgacaaaggt ggccgggtgc
    12541 accgtgttca tgcgcgccat ggtgccgttg gtggccacga tcacggccga aaaaccgggc
    12601 gaatccagca gcacattggc gcggcgcgcc tgaaccaccc aaaaatcgtc gtcgtcgtcg
    12661 ctgagtttga tggggtgcgg gtcgtcgccc gtgcgctcgc ggcggatgat gtccacctca
    12721 aagccgtctt tattgacagc ggtgtatttt tgacttttgc ggatgcggaa tgtgttgtcc
    12781 accttcttca gcacgcccag catggaactg tctaccttga caagctgggt tgaaaagagg
    12841 atgcgttttc gggtggccag cgtttcagag tccagtcgca cccctgcggt agcttcatag
    12901 gcgtacagcg cttgggtgcc taccacccga aagtgcgggc tcagttgggt gctgaccagg
    12961 cggcccagca gcgccaccac cagcgggtcc acccggccca tgcgcagggc gcggttcaag
    13021 cgttggtgtt gctccag
//
LOCUS       DAOSLL010000047        12763 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4971_length_12763_cov_4.590101, whole genome shotgun sequence.
ACCESSION   DAOSLL010000047 DAOSLL010000000
VERSION     DAOSLL010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 12763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12763
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4971_length_12763_cov_4.590101"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /gene="murJ"
                     /locus_tag="PLB25_03370"
     CDS             complement(<1..1212)
                     /gene="murJ"
                     /locus_tag="PLB25_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HOE40661.1"
                     /translation="MSLFKSASIVSLLTLLSRVTGLVRELLIASTFGASAMTDAFNVA
                     FRIPNLFRRFFGEGAFSQAFVPVLATCKAQQGEAATRVLIDRVATVLAWALLLLSVIG
                     VLGAAGLVWAMASGLKQEPRGFEVAVVMTRWMFPYIAFMSLVALGAGVLNTWKRFAVP
                     AATPVLLNVCMIAATWLGSPWFRSLGLEPIYALAGGVLLGGATQLAVQWLALKRMGLH
                     PRMGWRWSALRVAWADAGTQNILRLMGPALLGVSVAHISMLINTQIASHLRSGSVSWI
                     TYADRLMEFPTAMLGVAMGVVLMPQLASARANGDAAKYSGMLDWGLRLVAVLAVPCAV
                     ALLVFPTPLVAVLYHYGAMTEFDVQQVTYALMGWGVGLVGIVAIKVLAPGYYANQDTR
                     TPVRVAIGVLVL"
     gene            1321..1620
                     /gene="rpsT"
                     /locus_tag="PLB25_03375"
     CDS             1321..1620
                     /gene="rpsT"
                     /locus_tag="PLB25_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HOE40662.1"
                     /translation="MASGKPKKKNPRLASGRKRVRQDIKLNAANTSLRSQYRTAVKNV
                     EKALLTGDKAKATELFARMQSVVDIVADKGIFHKNKAARDKSRLSSKVKALAVAA"
     gene            complement(1768..2103)
                     /locus_tag="PLB25_03380"
     CDS             complement(1768..2103)
                     /locus_tag="PLB25_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3579 domain-containing protein"
                     /protein_id="HOE40663.1"
                     /translation="MVSHTSKKVYILGVTHSGRAFRPSDWAERLAGVMSQFRPGLARP
                     GSHIAYSPWCVPSSVNGIRCVIVDEKLRGHEVMAWDFCMDFAQDNELQVSHGAPPDPK
                     AVSRPPHST"
     gene            2440..3660
                     /locus_tag="PLB25_03385"
     CDS             2440..3660
                     /locus_tag="PLB25_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOE40664.1"
                     /translation="MSTAAHFIEAQSPHVMSTYGRLPIALSHGQGSRVWDVNGKCYLD
                     ALGGIAVNTLGHNHPRLVPALQDQIGKIIHSCNYYHVPNQEVLAKKLVELSGMSNVFF
                     CSTGLEANEAALKLARKFGHDKGIDRPEIVVYEHAFHGRSIATLSATGNPKIQAGFEP
                     LVEGFIRVPINNIESLKAATANNPNVVAVFFEAIQGEGGINPMQLDYLRELRDLCNGR
                     DWLMMIDEVQSGMGRTGKWFAHQWAGIVPDVMPLAKGLGSGVPVGAVVAGPKAAHIFK
                     VGNHGTTFGGNPLAMRAAVETIRIMEEDGLLAHAAQLGSHLSGALTQALAAELASGAV
                     KEIRGQGLMIGIDLDKPCGALVQQCADHGLLISVTADTVVRLVPPLILTEAEADEVVS
                     ILCPLISTLLKEPS"
     gene            3657..4631
                     /gene="argF"
                     /locus_tag="PLB25_03390"
     CDS             3657..4631
                     /gene="argF"
                     /locus_tag="PLB25_03390"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HOE40665.1"
                     /translation="MNPAGVGIPSTIPKGAPPVRHYLQFSDLSADEYRYLLQRAAFIK
                     SKFKTFTRHQPLVDRTLAMIFEKASTRTRVSFEAGMYQLGGSVVHLTTGDSQLGRAEP
                     IEDSARVISRMADLVMIRTFEQTKIERFAEYSRVPVINGLTNEFHPCQILADIFTYIE
                     HRGPIQGKTVAWVGDGNNMANSWLQAASLLDFKVNVSTPSGYEVNEKIASGPHGISAG
                     SYETYSDPMAACRAADLVTTDVWTSMGFEAENEVRKQAFAAWRVTTEMMAHAKPDALF
                     MHCLPAHRGEEVDADVIDGPQSVVWDEAENRMHVQKALMEYLLLGAIN"
     gene            4652..4843
                     /locus_tag="PLB25_03395"
     CDS             4652..4843
                     /locus_tag="PLB25_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40666.1"
                     /translation="MSDDKEPKLWVTAVKLAVGAIVLIALIGLGLYYTAEFNTYSPGQ
                     AEPSNSWGITKDSPIRSNR"
     gene            complement(4857..5087)
                     /locus_tag="PLB25_03400"
     CDS             complement(4857..5087)
                     /locus_tag="PLB25_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40667.1"
                     /translation="MTQTWMVGLLVGLAGLYSVWYLLPTAARKRLGRWNRWLASAPAC
                     GSCSECGNCAKPPAGNSPPGVAGQALTFHKKP"
     gene            complement(5089..6975)
                     /locus_tag="PLB25_03405"
     CDS             complement(5089..6975)
                     /locus_tag="PLB25_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HOE40668.1"
                     /translation="MTRHPEAGLPPLRVALLGNPNCGKTALFNLLTGSHQKVANYAGV
                     TVERKEGQLRTASGRLITVLDLPGAYSLNAMSADEQITRDIVTGHRPQEPLPDLVICV
                     VDATNLRLNLRLVLEARALQLPLVLALNMCDSADREGIRVDRAVLTRELDVTVLETVG
                     IRHDGAHELLDYLDQVQPVKPAGPTVAWHGSSVDQVMATQQEVRRIVRLAVQEPVDSL
                     HADDAIDAIALHPLWGMLLLAVTLFLMFQAVFSWATWPMDGIKAGLELLSGLLRTHLP
                     EGPLNSLLVDGVLAGAGGVMVFLPQILILFFFILVLEDSGYLPRAAFLLDRVMGTVGL
                     SGRSFIPLLSSFACAVPGIMATRTITHWRDRLVTIMIAPLMTCSARLPVYALLIGAFI
                     PARPVLGVFNLQGLVMFALYLGGIVSAMAVAGVSMVWRTQARSTPLVMELPSYRWPSL
                     RSLAHGLYERASIFIRRVGGIILALTVLLWFLSSYPGAPDGATGAAIEYSFAGRLGHW
                     LEFLVAPIGFNWQIAIALVPGMAAREVAVGALGTVYALSATGSDLASQLVPLIGNSWS
                     LGTALSLLVWFVFAPQCISTLVAVRRETNSWRYPLLMLFYLFGLAYLASFLTYRLTLM
                     WTGA"
     gene            complement(6977..7285)
                     /locus_tag="PLB25_03410"
     CDS             complement(6977..7285)
                     /locus_tag="PLB25_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - iron ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HOE40669.1"
                     /translation="MSPIKSHAEATSSPIGLHQLACHVPAEVLGMNPAHDDEERSVAL
                     RLLEIGFLPGESVRVIAHGFPGHDPLAVRVGHTTFALRSHEAALVQVRPTGQTQARRT
                     "
     gene            7758..12110
                     /gene="purL"
                     /locus_tag="PLB25_03415"
                     /gene_synonym="purI"
     CDS             7758..12110
                     /gene="purL"
                     /locus_tag="PLB25_03415"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HOE40670.1"
                     /translation="MTLHISQFEGGSALSAFRVQQLLPGLQAVHDKINGVAARYVHLV
                     ATLTEPSAALKEQLAALLVYGEPYLGAHDGALIVVSPRLGTVSPWASKATDIAHNCGL
                     ALRRIERVVEYRIALKSGLLSRPSLTAGQLRQIADLLHDRMTESVMFERAQALKLFDE
                     LPAAPMAQVDVLHGGKAALLAANTEFGLALAADEIDYLVTAFTQLQRNPTDVELMMFA
                     QANSEHCRHKIFNAAFTIDAVAQPDSLFGMIRHTHQQNPQHMLVAYSDNASVMAGAVV
                     QRFSAKSASGPSGVSAASYQKQSALEHILMKVETHNHPTAISPFPGAATGAGGEIRDE
                     GATGRGSRPKAGLAGFTVSKINWGQSRINSSDSKAENNSTLTPISSIHYGKPTHIASP
                     LQIMIDGPLGGAAFNNEFGRPNLLGYFREYEQALTYAVQDPQGKRIEQHEQRGYHKPI
                     MLAGGLGVIDASQTHKVAFPAGSLLIQLGGPGMRIGMGGGAASSMTTGANAAHLDFDS
                     VQRGNPEIQRRAQEVINQCWLQGTDDAQGGRGNPILAIHDVGAGGLSNAFPELTNDAG
                     RGARFDLRSVPLEESGMAPKEIWSNESQERYVLAIAPDSLPGFKALCERERCPFAVVG
                     VATEARDLIVGDVDVTQAAGAQGDGGGLPVSMPMDVLLGKPPKMQRDVTSIQRQFAPV
                     NLTGIALQQAAIEVLAHPSVACKRFLVTIGDRSVGGLSHRDQMVGPWQVPVADCAVTL
                     ADFAGFAGEAMAIGERTPLASVNAPASGRMAVSEAITNLLAAPIELDRVKLSANWMAA
                     CGEPGEDAALYATVKAVALELCPALGISIPVGKDSLSMRTQWTESADTGAGKAGKADA
                     NGASVTKKVTSPVSLIVTAFATLADVRGTLTPQLNATGDTSLILIDLGRGQNRMGGSI
                     LAQTINQMGDSVPDLDQPQDLINLVKVVNILRQRGQILAYHDRSDGGLFATVCEMAFA
                     GHVGVALNVDLLVTEGDGITDGRMDVGDGKNWASQVSARRDELTLKALFNEELGVVLQ
                     VCTAERNAVMQTLREHKLSQHSHVIGKTRPADSRIQVGVGAVQVWRDTKAIFSATLAD
                     LHQVWDATSWKICQRRDNPACADAEHASAGDPADPGLHIVLANSSGSAAAAQAGTLPA
                     AATVDATQSGPGAARQTNDVSASNQPTAHTGQAPTATQSIVIDGAPGLLLSRPKVAVL
                     REQGVNSHVEMAYAFTEAGFEAFDVHMTDLQTGRASLADFKGVVACGGFSYGDTLGAG
                     IGWARSITFNPVLADQFMAFFGRADTFGLGVCNGCQMFAELADIIPGAQDWPRFTTNQ
                     SERFEARFSMVEVLDSPSLFFTGMAGSRLPIAVAHGEGYANFKHRGDAAQVIAAMRFT
                     NNFGQATEAYPFNPNGSPGGLTAVTTADGRFTAMMPHPERVFRNIQMSWTDQPPDAFS
                     PWLQLWHNARAWVG"
     gene            12168..>12763
                     /locus_tag="PLB25_03420"
     CDS             12168..>12763
                     /locus_tag="PLB25_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HOE40671.1"
                     /translation="MSDRPAQWWAVLFLISAALAWVLTVLHLPAAVLLGCMLAAIVLS
                     SHGIRLAMPKPWFALAQGVLGCLMARSLQPATLVRLAADWPIFLGAAVSVIGASAGLG
                     WILMRRQVLPGTTVVWGLAPGAASAMVLMAEAHGADVRLVAFMQYARVVVVTLVASLV
                     TWVWGARAGAAASPVDWFAVHHASDLGATLLLAVAGSVA"
BASE COUNT         2633 a         3958 c         3847 g         2325 t
ORIGIN      
        1 caaaaccaac acgccaatgg ccacccggac cggggtcctg gtgtcctggt tggcatagta
       61 gcccggtgcc agcaccttga tcgccacaat gccgaccagc ccgacacccc agcccatcaa
      121 cgcataggtg acctgctgca catcgaattc ggtcatcgca ccatagtggt acagcactgc
      181 caccagcggt gtcggaaaaa ccagcagtgc cactgcgcag ggcactgcca gcaccgccac
      241 cagccgcagg ccccagtcca gcatgcctga gtactttgcc gcatcgccat tggcgcgggc
      301 actggccaac tgcggcatca gcaccacgcc catggctaca ccaagcatgg cagtagggaa
      361 ctccatcagc cgatccgcat aggtgatcca gctcacgctg cccgaacgca aatgtgaggc
      421 aatctgggtg ttgatcagca tcgagatatg cgccacgctg acccccagca gggcaggacc
      481 catcaggcgc aagatgtttt gcgtgcccgc atcggcccag gcgacgcgca gggctgacca
      541 acgccaaccc atgcgggggt gcagccccat gcgtttcaag gccaaccatt gcactgccaa
      601 ttgcgtggcc ccccccagca gcacgccacc tgccagcgcg tagattggct ccaggcccaa
      661 cgatctgaac cagggtgagc ccagccaggt ggccgcgatc atgcacacat tgagcagcac
      721 cggtgtcgcc gcaggcacgg caaaacgctt ccaggtattg agcacaccag cgcccagcgc
      781 caccagcgac atgaaggcaa tgtacggaaa catccagcgc gtcatgacga cggccacctc
      841 gaacccgcgt ggctcctgct tcaagccact cgccatagcc cacaccaggc cggccgcacc
      901 caggacaccg atcacgctga gcagcaacag cgcccacgcc agcaccgtag cgacccggtc
      961 aatcaacacc cgtgtcgcgg cctcgccctg ctgtgccttg caggtcgcca acactggcac
     1021 aaatgcctga ctgaaagcgc cttcaccaaa aaaacgcctg aacaaattgg gaatgcgaaa
     1081 cgcgacattg aatgcatcgg tcatggccga cgcgccaaac gtcgaggcaa tcagcaactc
     1141 gcgcaccaaa ccggtcacgc gcgacaacag cgttagcagc gagacgatgg aggcagattt
     1201 gaacaggctc acggcgcgaa gtgtagccgc gcctcaacca agttccggcg ctggtgtggc
     1261 tgccctgcta gaattgcgag ctttgctgac aacatccaca gactcaagga acacttatat
     1321 atggcatctg gaaaacccaa gaaaaagaac ccgcgcctgg catcggggcg caagcgcgtc
     1381 cgtcaggaca tcaagctcaa tgcagccaac acctcgttgc gctcgcaata ccgcacagca
     1441 gtgaaaaatg tcgaaaaagc attgctgaca ggcgacaagg ccaaagccac cgaactgttt
     1501 gcccggatgc aatccgtggt ggacattgtg gccgacaagg gaatcttcca caagaacaag
     1561 gccgctcgtg acaagagccg actatcgtcc aaggtcaaag ccttggccgt tgctgcctga
     1621 tattcaggca gatcgcacga attcctgagg gatcgggaat ttgccgtttg taacgggtgc
     1681 gctgtcagca aaagcaaaaa agccgtcgca agacggcttt tttgctttgc gcccggtccg
     1741 gcaccagtgc ctgggcgggt tgatcggtca ggtgctgtgg ggtgggcgac tcaccgcctt
     1801 ggggtcgggg ggcgcaccgt gcgacacctg caattcgttg tcctgggcaa agtccatgca
     1861 gaaatcccag gccatgacct catggccgcg caatttttcg tccacgatga cgcagcgaat
     1921 accgttgacg ctgctcggca cacaccaggg tgagtaggca atatgactac ctgggcgcgc
     1981 cagtccgggg cgaaactgac tcatcacgcc agccaggcgc tcagcccagt cgctcgggcg
     2041 aaacgcgcga ccactgtggg ttacgcccaa aatatagact tttttcgaag tatgtgaaac
     2101 catctggtag aggtccgtca atgggcgtga ttggaatgct ggaacgcagc aggatgttaa
     2161 cgcaatctta tgtcttataa aagacccgat tgaatgcctg agtttgagcc gacgtggtaa
     2221 atgcgagcca cggcaccagg ataaatttgt tcttttggca tcgtcgacat tcccgcctgc
     2281 gcgggaatgt cggtaaatca acgacttagc gaaagaataa atttgccctg acggcaccag
     2341 caagcgttgc gacagggcct gttgatcagt ctaaaatcat ctctcaacgg cccaacctgc
     2401 gttgggccgc attgtttttt gccgccggag aaactgccca tgtccactgc cgcccacttc
     2461 attgaagccc aatcgcccca tgtcatgagc acctatgggc gattgccgat agcgttgtcg
     2521 catggccagg gcagccgggt gtgggatgtc aacggcaaat gttatctgga tgcgctcggt
     2581 ggtattgccg tcaacacgct tggacacaac cacccgcgtc tggtgcctgc gctgcaggat
     2641 caaatcggca agatcatcca cagttgcaac tactaccacg tgcccaatca ggaagtgctg
     2701 gccaaaaaac tggttgaact atcgggcatg agcaacgtgt ttttttgctc taccgggctc
     2761 gaagccaatg aggccgcgct aaagctggcg cgcaagttcg gccatgacaa gggcattgac
     2821 cgccccgaaa tcgtggttta cgaacatgcc tttcatggcc gcagcattgc caccctgagc
     2881 gccactggca acccgaagat ccaggccggt tttgagccgc tggtggaggg ctttatccgg
     2941 gtaccgatca acaacatcga aagcctgaaa gccgccaccg ccaacaatcc caatgtggta
     3001 gccgttttct ttgaagcaat tcaaggcgaa ggcggcatca acccgatgca gcttgactac
     3061 ctgcgtgagc tgcgcgatct gtgcaatggg cgcgactggc tgatgatgat tgacgaggtg
     3121 cagtccggca tggggcgcac cggcaaatgg tttgcccatc agtgggctgg cattgtcccg
     3181 gacgtgatgc cactggccaa aggactgggg tccggggtgc cggtgggtgc tgtggtggcc
     3241 gggcccaaag ccgcccatat tttcaaggtg ggcaaccacg gcactacatt tggcgggaac
     3301 ccgctggcca tgcgcgccgc agtcgagacc atccgcatca tggaagaaga cggcttgctg
     3361 gcgcacgccg cgcagctcgg cagccacctg tcaggcgcat tgacgcaagc acttgctgcc
     3421 gagctggctt cgggtgcggt caaggagatt cgcggtcagg ggttgatgat tggcattgac
     3481 ctggacaaac cttgtggcgc gctggttcaa caatgcgccg accacggcct gctgatcagt
     3541 gtcaccgccg acacggtggt gcgactggtg cccccgctga tcctcaccga ggccgaggcc
     3601 gacgaggtgg tcagcatttt gtgtcccctg atttccaccc tgttgaagga gccctcatga
     3661 accctgccgg cgttggtatc cccagcacca ttcccaaagg tgcgccgccc gtgcggcact
     3721 atttgcaatt ttcggacctg agcgctgacg aataccgtta cctgttgcag cgcgcggctt
     3781 tcatcaagtc caagttcaag accttcaccc gccatcagcc gctggtggac cgtacgctgg
     3841 cgatgatttt tgagaaagcc tcgacccgca cccgggtcag tttcgaggct ggcatgtacc
     3901 agttgggcgg cagcgtggtc cacctgacca ccggcgacag ccagttgggc cgggccgaac
     3961 cgatcgaaga cagcgcccgg gttatcagcc gcatggccga cctggtgatg atccgcacct
     4021 ttgagcagac caagatcgag cgctttgccg agtattcgcg cgttccggtc atcaatggtc
     4081 tgaccaatga gtttcacccc tgccagattc tggctgacat cttcacctac attgagcacc
     4141 gtggaccgat ccagggtaaa actgtggcct gggtgggcga tggcaacaac atggccaact
     4201 cttggctgca ggccgcctcg ctgctcgact tcaaggtcaa cgtgagcacc cccagcggtt
     4261 acgaagtgaa tgagaaaata gcctctggcc ctcatggaat aagcgctggt agctatgaaa
     4321 cctatagtga tccgatggct gcctgccggg ctgccgacct ggtcaccacc gatgtctgga
     4381 ccagcatggg gtttgaagct gaaaacgagg tgcgcaaaca ggcttttgcc gcctggcgag
     4441 tcaccaccga gatgatggcg catgccaagc cagacgcgtt gttcatgcat tgcctgcctg
     4501 cgcaccgcgg cgaagaggtc gatgccgatg tcatcgacgg cccgcaaagc gtggtctggg
     4561 atgaggccga aaaccgcatg cacgtgcaaa aagccttgat ggaatacctc ttgctagggg
     4621 caatcaactg atcattgaac gaggtgcgct gatgagtgat gacaaagaac ccaaactgtg
     4681 ggtgacagcg gtgaaactgg cggtgggtgc cattgtgctg atcgcactga tcggcctggg
     4741 actgtattac accgccgagt tcaacactta ttcgccaggg caagcagagc ccagcaattc
     4801 ctggggcatt accaaagact cgccgattcg cagcaatcgt tgagccagtc agcccatcag
     4861 ggttttttgt gaaatgtcag ggcctgaccc gctacgcccg gcgggctgtt gcctgccggt
     4921 ggtttcgcac agttgccgca ttcgctgcaa gaaccgcaag ccggtgcact tgccagccag
     4981 cggttccagc ggcccaggcg ttttctggcc gcagttggca gcaaatacca aacgctgtaa
     5041 agcccagcca ggccaaccag caggcccacc atccacgtct gcgtcacgtc atgctcctgt
     5101 ccacatcaag gttaaacgat aggttaaaaa actggccaga taggccaggc caaacaggta
     5161 aaacagcatc aacagcgggt aacgccacga attggtttca cgccgcacag cgaccagcgt
     5221 cgagatgcac tgcggcgcaa atacaaacca gaccagcagc gacagcgcgg tgcccaaaga
     5281 ccagctattg ccgatcaggg gaacaagctg cgaggcaagg tccgagcccg tcgccgacaa
     5341 agcataaaca gtacccagcg cacccacggc gacctcgcgc gccgccatgc cgggcaccag
     5401 cgcaatggca atttgccagt tgaacccgat cggtgccaca agaaactcca gccaatgccc
     5461 aaggcggcca gcaaaactgt actcgattgc cgcgccggtg gctccgtccg gtgcacccgg
     5521 ataggacgac agaaaccaca gcagcaccgt cagtgccagg atgatgccac cgacccgtct
     5581 gatgaaaatt gacgcacgtt cgtacaggcc gtgcgccagg ctgcgcaaac tcggccagcg
     5641 gtaggatggc aattccatca ccagcggggt tgaccgggcc tgggtgcgcc acaccatcga
     5701 aacacccgcc accgccatcg ccgacacaat gccgcccagg tacagcgcaa acatcaccag
     5761 cccctgcagg ttgaacaccc cgagcaccgg gcgcgccgga atgaaggccc cgatcagcag
     5821 tgcatacacc ggcaaacggg ccgagcaagt catcaaaggc gcaatcatga tggtcaccag
     5881 ccgatcacgc cagtgcgtga tggtgcgtgt ggccatgatg cctggcaccg cgcaggcaaa
     5941 gctcgacaac agtggaatga acgagcgacc tgacaagccg accgtgccca tcacccgatc
     6001 cagcaagaac gccgcgcgcg gcaggtagcc ggagtcttcc agcaccagaa tgaagaaaaa
     6061 cagaatcagg atttgcggca aaaacaccat cacgccgccg gccccggcca gcacgccgtc
     6121 caccagcagg ctgttcaacg gcccttccgg caggtgggtc cgcagcaggc ctgacaacag
     6181 ttccaggcca gccttgatgc catccatcgg ccaggtggcc caactgaaca ccgcctgaaa
     6241 catcaaaaac agcgtgacgg ccagtagcag catgccccac agcggatgca aggcaatggc
     6301 atcaatcgcg tcatcggcgt gcaggctgtc gaccggctcc tgcaccgcaa gcctgacaat
     6361 gcgcctgacc tcttgctgcg tcgccatgac ctgatccacc gacgagccat gccaggcgac
     6421 tgtcggcccg gctggtttga ctggctgcac ctggtccagg tagtccagca gttcatgtgc
     6481 accgtcgtgg cggataccga cggtttccag aaccgtcaca tcgagttcgc gggtcaacac
     6541 cgctcggtcc acgcggatgc cctcgcggtc ggccgaatca cacatgttga gcgccagcac
     6601 cagtggcaac tggagtgcgc gggcttccag caccaggcgc agattgagcc gcaggttggt
     6661 ggcatcaacc acgcagatca ccaggtcagg cagcggctcc tgaggccgat gcccggtgac
     6721 gatatcgcgg gtgatctgct cgtcggcgct catcgcattc aggctgtagg cacccggcag
     6781 atcaagcacc gtgatgagcc gccccgaggc ggtgcgcaat tgcccctcct tgcgttcgac
     6841 cgtcacgccg gcgtagttgg cgaccttctg gtggctgccg gtcagcaagt tgaacagcgc
     6901 cgtcttgccg caattgggat tgcccagcaa ggcaacgcgc aggggtggca ggccagcttc
     6961 agggtgacgc gtcatgtcac gtcctgcggg cctgggtttg accggttggc cgcacctgaa
     7021 ccagcgccgc ttcatggctg cgcagcgcaa aggtggtatg accgacgcgc accgcaagcg
     7081 ggtcatggcc gggaaagccg tgcgcaatca cccgaaccga ttcacctggc aaaaacccga
     7141 tttccagcaa acgcagtgcc acactgcgct cttcatcgtc gtgtgccgga ttcatgccga
     7201 gcacctcagc cgggacatgg caagccaatt gatgcagtcc gattggcgaa gaagtcgctt
     7261 cagcgtgtga tttgatcggc gacataaacc accttaaatg agaacagttc ttattgtaac
     7321 aaagaagatc cgatgccaat ccggttcgct gttcaagttc tgaaaacggc cggtctgttg
     7381 cgggccgatg acaaattgtc cgcgtcggcg cttcccggat cttcggctgt gaccactgct
     7441 gccgctttgg cagacccgca ttggcaagca tccggatggg ccgcaagcac cgtctgccgg
     7501 gcgtcagtga ggcaagcttg gggtggtttt gaaaaaggcg cagccgacag cgtgacacca
     7561 ggtgtgccgc ggaatggcaa acttcagcaa accctcaggc atttcaaggc gccgatgccg
     7621 acttgcatgc agcgtcatct gccaagcatg ggtggcacgg ctggcgctgg taaaattttt
     7681 gacctcactg ccaaaccacc tgccatgctg gtcgcgcgtc aatttcaacc tcgtcagcac
     7741 ctcaacaagt cctcaccgtg accctgcata tctcccaatt cgagggcggc agcgctctca
     7801 gcgcctttcg tgtccaacag cttttgcccg gcctgcaagc agtgcatgac aagatcaacg
     7861 gcgttgccgc gcgctatgtg catctggtgg ccacgctgac cgagccgtcg gcggcgctca
     7921 aagagcagtt ggccgccttg ctggtctatg gcgagcctta cctgggtgcg cacgacggcg
     7981 cgttgattgt ggtgtcgccg cgcctgggta ccgtgtcgcc ctgggcctcc aaagccaccg
     8041 atattgcgca caactgcggc ctggcgctac gccggatcga gcgggtggtg gaataccgta
     8101 ttgccttgaa aagcggcctg ctgtcgcggc ccagcctgac tgccggacaa ctccgccaga
     8161 tcgccgacct gctgcacgac cgcatgaccg agagtgtgat gtttgaacgt gcccaagcct
     8221 tgaagctgtt tgacgaactg cccgccgccc cgatggcaca ggttgacgtg ttgcatggcg
     8281 gcaaagcagc actgctggcc gccaacaccg aatttggctt ggcgctggcc gctgacgaaa
     8341 tcgactacct ggtcaccgcc ttcacccagt tgcagcgcaa cccgaccgat gttgagctga
     8401 tgatgtttgc ccaggccaac agcgaacact gccgccacaa gatattcaac gccgcgttca
     8461 ccatcgacgc tgtggcgcaa cccgacagcc tgtttggcat gatccgccac acccaccagc
     8521 aaaacccgca gcacatgctg gtggcgtatt cggacaacgc ctcggtgatg gccggtgcag
     8581 tggttcagcg ctttagtgcc aaatctgcct ctggcccaag tggtgtaagc gctgccagct
     8641 atcaaaaaca aagtgctctg gagcacatcc tgatgaaggt ggaaacccac aaccacccga
     8701 cggcgatctc gccctttccc ggtgctgcca ccggagccgg tggcgaaatc cgcgacgaag
     8761 gtgccactgg ccgtggctcc agacccaaag ccggcctggc cggtttcacc gtgtcgaaaa
     8821 ttaattgggg tcagagtcga attaattcat cagattcaaa agccgaaaat aattcgactc
     8881 tgaccccaat tagttcaatt cattacggca agccgacgca cattgccagc ccgctgcaga
     8941 tcatgatcga tgggccgctg ggcggtgcgg cgttcaacaa tgagtttggg cgaccgaatt
     9001 tgctggggta ttttcgcgag tacgagcagg ccttgaccta cgcggtgcaa gacccgcaag
     9061 gcaagcgcat cgaacagcac gagcagcgcg gttaccacaa accgatcatg ctggcgggcg
     9121 ggctgggcgt gattgacgcg agccagacgc acaaggtggc tttcccggct ggcagcctgc
     9181 tgatccagtt gggtgggccg ggcatgcgca ttggcatggg tggtggcgcg gccagttcca
     9241 tgaccaccgg ggccaacgcg gcgcatctgg actttgactc ggtgcagcgt ggcaaccccg
     9301 aaatccagcg ccgtgcgcaa gaggtcatca accaatgctg gctgcagggc acggacgatg
     9361 cccaaggcgg gcgcggcaat ccgattctgg caattcacga tgtcggtgcg ggcggtttga
     9421 gcaacgcttt tcccgagctg accaacgacg ctggccgtgg cgcgcgcttt gatttacgtt
     9481 cggtgccgct ggaagagtct ggcatggccc ccaaggaaat ctggtcgaac gagagtcagg
     9541 agcggtatgt gctggccatc gcgcccgact cgctgcccgg cttcaaggcc ttgtgcgagc
     9601 gcgagcgctg cccgtttgcg gtggtcggcg tggcgaccga ggcgcgcgat ttgatcgtcg
     9661 gcgatgtcga tgtgacgcag gccgccgggg cgcagggtga tggcggcggt ttgccggtca
     9721 gcatgccgat ggacgtgctg ctcggcaagc cgcccaaaat gcagcgcgat gtgacatcga
     9781 ttcagcgcca gtttgcgccg gtcaacctga ccggcatcgc cctgcaacag gcggccatcg
     9841 aggtgctcgc gcatcccagc gtggcttgca agcggtttct ggtgacgatt ggcgaccgca
     9901 gcgtcggtgg cctgagccat cgcgaccaga tggttggccc gtggcaggtg ccagtggccg
     9961 actgcgccgt gacgctggca gactttgccg gctttgccgg tgaggccatg gccatcggcg
    10021 agcgcacccc gctggcctcc gtcaatgcac cggcttccgg gcgcatggcg gtcagcgagg
    10081 ccatcaccaa tttgctggcc gcaccgatcg agcttgaccg cgtcaagctg tcggccaact
    10141 ggatggccgc ctgtggcgaa cccggtgaag atgccgcgct gtatgcaacc gtcaaggccg
    10201 tagcgctgga actgtgcccg gcgctgggga tttcgattcc ggtcggcaag gattcgctct
    10261 ccatgcgcac gcagtggact gaatcggcgg acaccggcgc tggcaaggct ggcaaggcgg
    10321 atgcgaatgg cgcttcggtg accaaaaagg tcaccagtcc ggtgtcgctg atcgttaccg
    10381 cctttgccac cctggccgac gtgcgcggca cgctgacacc gcaactcaac gcgaccgggg
    10441 atacttcgct gatcctgatc gacctgggcc gcggccaaaa ccgcatgggc ggcagcatcc
    10501 tggcgcaaac catcaaccag atgggcgact cggtgcctga tctggaccag ccgcaagacc
    10561 tgatcaatct ggtcaaggtg gtcaacatct tgcgccagcg cgggcagatt ctggcctacc
    10621 acgaccgcag cgacggcggc ctgtttgcca ccgtttgtga aatggcgttt gccggtcatg
    10681 tcggcgtggc gctgaatgtg gacctgctgg tgaccgaggg cgacggcatc acggacggtc
    10741 ggatggatgt cggcgatggc aaaaactggg cctcgcaggt cagtgcacgg cgcgacgagc
    10801 tgaccctgaa agccctgttc aacgaagaac tcggcgtcgt gctgcaggtg tgcaccgctg
    10861 agcgcaatgc cgtgatgcaa accctgcgcg aacacaagct gagtcagcac agccatgtca
    10921 tcggcaaaac ccgcccggct gattccagga ttcaggtcgg ggtcggcgca gtgcaggtat
    10981 ggcgcgatac caaggccatc ttcagcgcca ccctggccga cctgcaccag gtgtgggatg
    11041 cgacaagctg gaaaatctgc cagcggcgcg acaacccggc ctgtgccgat gccgaacacg
    11101 ccagcgccgg tgatcctgcg gacccgggcc tgcacattgt gctggccaat tccagcggct
    11161 ctgccgctgc cgcgcaggct ggaaccctgc ccgctgctgc aaccgtggac gccacccaaa
    11221 gcgggcctgg cgcggcgcgg caaacaaatg atgtttcagc atcaaatcag cctacagccc
    11281 atacgggaca agcgccaaca gctactcaat caatagtgat tgatggcgct cctgggttgc
    11341 tgctgtcgcg ccccaaggtg gccgtgttgc gcgagcaggg ggttaattcg catgtcgaga
    11401 tggcctacgc gtttaccgaa gccgggtttg aagccttcga cgtgcacatg accgacctgc
    11461 aaaccggtcg cgccagcctg gccgacttca agggtgtggt ggcctgtggc ggcttcagtt
    11521 atggcgatac gctcggtgct ggtattggct gggcgcgctc gatcaccttc aacccggtgc
    11581 tggctgacca gttcatggcc ttttttggcc gcgctgatac cttcggcctg ggtgtctgca
    11641 atggctgcca gatgtttgcc gagctggccg acatcattcc cggtgcgcaa gattggccac
    11701 gcttcaccac caaccagagc gaacgctttg aggcgcgttt cagcatggtc gaggtgctcg
    11761 attccccatc attgtttttc accggcatgg caggcagccg cctgccgatt gcggtggcac
    11821 acggcgaggg ctatgccaac ttcaagcacc ggggcgatgc ggctcaggtg attgccgcga
    11881 tgcgctttac caacaatttc ggccaggcca ccgaagccta cccgttcaac cccaacggca
    11941 gccccggcgg cctgacggcg gtgaccaccg cagatggccg cttcactgcc atgatgccgc
    12001 accccgagcg ggtgtttcgc aacatccaga tgagctggac cgatcagccc cccgacgcct
    12061 tcagtccgtg gctgcagttg tggcacaacg cacgggcctg ggttggctga ttcgggcacg
    12121 ctctgtgctg gctatgcgtg accatcggga cgactttggc atcgcgggtg agtgaccgcc
    12181 cggcccaatg gtgggcggtg ctttttctga tcagtgcagc ccttgcctgg gtgttgaccg
    12241 tcttgcactt gcccgctgca gtgctgctgg gctgcatgct ggccgccatt gttctgtcct
    12301 cacacggtat ccggctggca atgcccaagc catggtttgc actggctcaa ggggtgctgg
    12361 gctgcctgat ggccagaagc ctgcagccgg cgaccctggt caggctggcc gctgactggc
    12421 cgatttttct gggggctgcg gtgtcggtga tcggtgccag cgccggactc ggctggatcc
    12481 tgatgcgccg ccaggtcttg cctggtacca ccgtggtgtg ggggctggca cccggtgctg
    12541 cctcggccat ggtgctgatg gcagaagccc atggtgccga tgtccgtctg gtggcattca
    12601 tgcagtacgc gcgggtggtg gtggtcacgc tggtggcctc gctggtaact tgggtctggg
    12661 gtgcccgcgc tggcgctgcg gccagtccgg tggactggtt tgcggtccac catgccagcg
    12721 acttgggcgc gacgctgctg ctggctgttg cgggttcggt ggc
//
LOCUS       DAOSLL010000048        12691 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_4997_length_12691_cov_4.759259, whole genome shotgun sequence.
ACCESSION   DAOSLL010000048 DAOSLL010000000
VERSION     DAOSLL010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 12691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12691
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4997_length_12691_cov_4.759259"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="PLB25_03425"
     CDS             complement(<1..1335)
                     /locus_tag="PLB25_03425"
                     /inference="COORDINATES: protein motif:HMM:NF019436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40672.1"
                     /translation="MAKQAKHPFHPIIYVRGFAATQGEIEETVADPYMGFNIGSTKAR
                     MAWTGDVKRFYFESPLVRLMTDHSYQDVFEDGEDLVFSDRTDRPLPYRCVVIYRYYDE
                     ASKDFGSGDTPPIEQFARGLDKLILRLRDKVCANLKNDVAPADFRVYLVAHSMGGLVC
                     RAFLQNPALGSAQARGAVDKVFTYATPHNGIDMRIVRNVPGWLTFGDINNFNRERMAG
                     YLALAPGDDVSVVRNFAPQRIFNLIGTDARDYSVAQGLSAWAVGEASDGLVRIDNAST
                     HGPGPDGSDIASPRAFVHRSHSGHYGIVNSEEGYQNLTRFLFGELRVDGFLDVDDISL
                     PVELDRAMQDGKDLHASYQFEVAASVRGCQWQMTRREVRENSAIFRTYSELFPGARGT
                     KRLPDRSRSPHLFSVFLDPAKSVKASGSVSFAFDLKVLVPDYEVDGLLFMKRH"
     gene            complement(1763..1981)
                     /locus_tag="PLB25_03430"
     CDS             complement(1763..1981)
                     /locus_tag="PLB25_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40673.1"
                     /translation="MDPAEGRVWIETLMAAYRLIRSLPVSPASKGGRKLKRIGQPQRD
                     PENQIKSGSRPRPDCAGSYFIDSKDNQH"
     gene            complement(2102..2326)
                     /locus_tag="PLB25_03435"
     CDS             complement(2102..2326)
                     /locus_tag="PLB25_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40674.1"
                     /translation="MASAMKPTNGTIPTGYVSPFKRLSMNGIHDDLLACAATLCSKGM
                     EDVKKIAVTLGTRANGPDGHPNCSILVAIT"
     gene            complement(2838..2930)
                     /locus_tag="PLB25_03440"
     tRNA            complement(2838..2930)
                     /locus_tag="PLB25_03440"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2894..2896),aa:Ser,seq:gct)
     gene            complement(2974..4242)
                     /locus_tag="PLB25_03445"
     CDS             complement(2974..4242)
                     /locus_tag="PLB25_03445"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HOE40675.1"
                     /translation="MSLIVHKYGGTSMGSTERIANVAKRVAKWARAGHQMVVVPSAMS
                     GETNRLLALAKELAPGKPTEAYNRELDMLAATGEQASSALLAIALQAQGLESVSYAGW
                     QVPIRTNSAYTKARIESIDDQKVRADLDAGRVVIVTGFQGLDEQGNITTLGRGGSDTS
                     AVAVAAAMKADECLIYTDVDGVYTTDPRVVPEARRLSTVSFEEMLEMASMGSKVLQIR
                     SVEFAGKYKVKLRVLSSFTPWDIDIVEEAKSGTLITFEEDENMEQAIVSGIAFTRDET
                     KIAVLGVPDKPGIAYQILGAVADANIEVDVIIQNVSKDGRTDFSFTVNHGDYAKAMEL
                     LKTVVVPKLGALEVLGDTKICKVSIVGIGMRSHVGVAATMFRVLSEEGINIQMISTSE
                     IKTSVVIDEKYMELAVRSLHKAFGLDTPPV"
     gene            complement(4242..5219)
                     /gene="tilS"
                     /locus_tag="PLB25_03450"
     CDS             complement(4242..5219)
                     /gene="tilS"
                     /locus_tag="PLB25_03450"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HOE40676.1"
                     /translation="MTQSLPQAMAAFSPELPLAVAFSGGADSTALLLACVQKWPGQVQ
                     AFHVHHGLQAAADDFEQHCKAFCAGLGVPLRVQKVDARHASGQSPEAAARHARYKAFE
                     ALALSDQALPAIQSIAIGQHADDQVETLLLALSRGSGLPGLSAMPPSWVRGRLRYHRP
                     LLQVSSAEIRRWLAGQAVAFIDDPTNLDERFTRNRIRARLLPALQACFPQFRDTFARS
                     AAHAAQAQSLLLELAALDLAQAGVPPNIKALQSFSGPRLTNVLRHWLQSAHQVAPSAA
                     QLNELMRQIRACQTRGHALHLKVANGFCIRRGTLLHWYNPAVLTPSSSP"
     gene            complement(5306..6280)
                     /locus_tag="PLB25_03455"
     CDS             complement(5306..6280)
                     /locus_tag="PLB25_03455"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HOE40677.1"
                     /translation="MAKKTFLDFETPIADLENKIEELRYVQTESAVDISAEINQLAKK
                     SQQLTKDIYSDLSPWQITKIARHTERPYTLEYLSEIFTHFIELHGDRHFADDPSIIGG
                     LARFNGTPCMVIGQQKGRDTKERGLRNFGMSKPEGYRKALRLMKTAEKFKLPLFTFVD
                     TPGAYPGIDAEERGQSEAIGRNIFEMAQLEVPIIVTIIGEGGSGGALAISVGDQVLML
                     QYSIYSVISPEGCASILWKTSDKAQEAADALGITAHRLKALGVIDKIVNEPVGGAHRD
                     HKQMAAFLKRALTDAFRQVSDLKTQELLDRRYARLQSYGRFTDTKPGK"
     gene            complement(6493..7143)
                     /locus_tag="PLB25_03460"
     CDS             complement(6493..7143)
                     /locus_tag="PLB25_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HOE40678.1"
                     /translation="MIAIDNIASCAPLPAYWQDACKHLMKKDRVMKRLIPQFGKACLQ
                     SRGDAFVTLARSVVGQQISVKAAQTVWDRFAKLPGQMTPAQVLLLKIDDMRAAGLSVR
                     KVEYLVDLALHFDNGKVHVDEWTGMDDEAIIDELVTIRGIGRWTAEMFLIFHLMRPNV
                     LPLDDVGLINGISKCYFSGDVVSRSDAREVALAWAPYRTVATWYIWRSLDPLPVTY"
     gene            complement(7314..8705)
                     /gene="cysS"
                     /locus_tag="PLB25_03465"
     CDS             complement(7314..8705)
                     /gene="cysS"
                     /locus_tag="PLB25_03465"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HOE40679.1"
                     /translation="MSLSIFNTLTRELEPFSPLQAGHVRMYVCGMTIYDLCHIGHARM
                     MMAFDVVQRWLKSSGYRVTYVRNITDIDDKIIKRAVERGITIRALTDEMIDAMHTDIA
                     RLGIEAPTLEPRATEYVPQMLKLISRLQARGLAYQAASGDVNFAVRKFPGYGKLSGKS
                     LDELRAGERVAVADGKDDPLDFVLWKSAKESEPDDAKWDGNAQGYGYAIGRPGWHIEC
                     SAMSCAALGETFDIHGGGADLQFPHHENEIAQSEGASGQPLAKFWVHNGFVRVDNEKM
                     SKSLGNFFTIREVLTRYDPETVRYFLVRTHYRTALNYSDVHLDDARGSLKRLYTALNL
                     VPPEAVAIDWTEPFAARFKVAMDNDFGTPEAVAVLFDLASEVNRTRSSSAAGLLKALG
                     GCLGLLQGEPKAFLQAGAGVDEAAIAQRIAARAAAKAAKDFALADRIRQELLAQGVVL
                     KDTASGTSWEVLQ"
     gene            8894..9541
                     /locus_tag="PLB25_03470"
     CDS             8894..9541
                     /locus_tag="PLB25_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE40680.1"
                     /translation="MKQTRSLVTCALRLLTLVAAMTVFSGHADEVADVAQLLRSKQFA
                     SALTLADRYLADKPADPQMRFFKGVIQRNLGQQAEAIVTFTQLTEDYPELPEPHNNLA
                     ALYAETGQIDKARLALEMAVRTNPNYAIAQENLGDIYARLASQAYAKAGQLDRSNPAV
                     PRKLALIGEIFKPALPGSRPVASAAAAAGSAPMPAAPASQPATATVTPTGNPAVR"
     gene            9793..10392
                     /locus_tag="PLB25_03475"
     CDS             9793..10392
                     /locus_tag="PLB25_03475"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE40681.1"
                     /translation="MKFRSFSTLALTALASLAIFSIVSPASAQAAPQVKFATSAGDFV
                     VEVYPDKAPKTVENFLQYVKDKHYDGTIFHRVIPNFMIQGGGFDAKYLQKPTRAPVPH
                     EGREALEKGGPRNVVGTVAMARTNDPQSATAQFFINVKDNGFLDPSAQSFGYTVFGKV
                     ITGFDVVEKIKLTPTGAGGPFATDVPKTPILINTATLVK"
     gene            10396..10902
                     /locus_tag="PLB25_03480"
     CDS             10396..10902
                     /locus_tag="PLB25_03480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE40682.1"
                     /translation="MSNPQVELHIANYGTITLELDQDKAPNTVTNFLSYVSKGHYDNT
                     IFHRVIPGFMVQGGGMEPGMKEKKGGQPIQNEANNGLKNQSYTVAMARTGDPHSATAQ
                     FFINVADNGFLNHTAPSAQGWGYAVFGKVVAGAEVVDKIKAVKTAKKGFHDDVPVEDV
                     VIEKAVAL"
     gene            10917..11765
                     /locus_tag="PLB25_03485"
     CDS             10917..11765
                     /locus_tag="PLB25_03485"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOE40683.1"
                     /translation="MNTTAHPPLANGRSRPVWPQLAAPRRWQSVDVISDLHLHAGDMP
                     TFSAWQNYMATTPADAVFILGDLFEVWVGDDVLAAPSGRSLGFEAHCQQVLASAAQRL
                     SLYFMHGNRDFLLGDGYAHGACMTLLSDPTVLTFDAQGWLLTHGDALCLADADYQQFR
                     ATVRTAAWRDEFLARPLTERHRIARSLRTQSESIKAAGAPVAEIDRASACDWLRAAGA
                     GNLVHGHTHRPDDEQLDDALADTSAPLHRWALSDWDANARPPRLQVLRLSVAQQPRGI
                     TLPSGF"
     gene            complement(11754..>12691)
                     /locus_tag="PLB25_03490"
     CDS             complement(11754..>12691)
                     /locus_tag="PLB25_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOE40684.1"
                     /translation="GDGQAIRSAMAVLDAALRGQAQAQAAVAATAEKSDENVPPAQSP
                     SAQDATESVAAEAPLGAADAAADAADAAATAEPAAEPPKPTVVRKPVVAMRGDDRPGM
                     RKEAPAMAGRGGKFGDRKDGPRTGGRDVGTRGPGRADERGSRPERADRAPMGERFDAP
                     RLGDAAFRAQRDALEQAQMALRRLSAQAHGEALTHLLQAWEQRDAALVPSVQELGNRV
                     SPAVRTAWVKALGQTPTEAASTALLRLEMAAEVPTPAEYLSDRRMLQLQLLTRRNDPP
                     PAQTWGQDAASVLGSAFDASQSRRLQNVLKLLLKP"
BASE COUNT         2591 a         3885 c         3658 g         2557 t
ORIGIN      
        1 atgacgtttc atgaacagca ggccatcaac ctcgtagtcc ggcaccagca ccttgaggtc
       61 gaaggcaaaa ctgaccgagc ccgaggcctt gacgctcttg gcagggtcga ggaacaccga
      121 gaacaggtgc gggctgcggc tgcgatcagg caggcgcttt gtgccgcgcg cgccggggaa
      181 aagttcggaa taggtgcgaa agatcgccga gttttcacga acctcgcgcc gcgtcatctg
      241 ccactggcaa ccgcgcacgc tggcggccac ctcgaactgg tacgaggcgt gcagatcctt
      301 gccgtcctgc atcgcccgat caagctcgac cggcagcgaa atatcgtcca catccaggaa
      361 gccgtcaacg cgcaattcac caaacaggaa acgcgtcagg ttctggtatc cctcctcgga
      421 attgacaatg ccgtagtgcc cggaatggct gcgatgcacg aatgcccgtg gcgaggcgat
      481 gtcgctgccg tccggccccg gcccatgggt gctggcattg tcgatgcgaa ccagcccgtc
      541 gctggcctcg ccgaccgccc aagcggaaag gccctgtgca accgagtaat cgcgtgcgtc
      601 ggtgccgatc aggttgaaga tgcgttgcgg cgcaaagttg cgcaccaccg agacatcatc
      661 gccaggtgcc agcgccagat agccagccat ccgctcgcgg ttgaaattgt tgatgtcgcc
      721 aaatgtcagc cagcccggca cattgcgcac gatgcgcatg tcgatgccat tgtgcggtgt
      781 cgcgtaggtg aagaccttgt ccaccgcgcc acgcgcctgg gcactgccaa gcgccgggtt
      841 ctgcaaaaag gcgcggcaaa ccagaccgcc catcgagtgg gccaccaggt acacgcgaaa
      901 atctgccggt gccacatcgt tcttcaggtt ggcgcagacc ttgtcgcgca gccgcaggat
      961 aagcttgtcg agaccacgcg cgaactgctc gatgggtggc gtatcgcccg aaccgaaatc
     1021 cttgctggct tcgtcgtagt agcggtaaat aacaacacag cgatagggca gcggccggtc
     1081 agtgcggtcg ctgaaaacga gatcttcgcc gtcttcgaag acatcctggt aactgtggtc
     1141 agtcatcagc cggaccagcg gcgattcaaa gtaaaagcgc ttgacatcgc cggtccacgc
     1201 catgcgcgcc ttggtcgaac cgatgttgaa gcccatgtag ggatcggcga cggtttcctc
     1261 gatttcgccc tgcgtcgctg cgaagccacg gacgtagatg atcgggtgaa aggggtgctt
     1321 tgcttgcttt gccatggcag gtctccggtg aaagctgcgc aacgcccaac aggcacagaa
     1381 tccagcgcca aacatcacgc aacgggtgga aatttcaatc agatgaggca aagtatcaca
     1441 ccgtgacgta gcccccgtgc cgcgtgaaaa ccctcaaatt gcagactggc tggcaggttt
     1501 gccgccaacg aaggtgcttt cgggattgct tccgggaatg ctttcgggtg cgccgggcaa
     1561 gccccgcagg gatgccccgc ggcggggccg gaattgccga cttggccgtt tgccagttga
     1621 cgcgctgatc aagccttcat gcgggtgcgg gttgcgcttg cagagcacat tcatgcgcat
     1681 ttggtcagcg cacgtctgac ttggcagcgg gaagacggcg ccgggtttgc cacggccgtc
     1741 gccgccgctt gccgtggcga gttcagtgct ggttatcttt actatcaata aaatagctgc
     1801 cagcgcaatc aggacggggt ctggagccac tttttatctg attttcaggg tcacgttggg
     1861 gttgaccaat gcgtttcaat ttgcgcccgc ctttgctggc gggcgaaacc ggcaacgacc
     1921 tgatcagcct gtatgccgcc atcaatgttt caatccacac ccggccttca gcagggtcca
     1981 aatggtggtt tgcaaatgca gtttaaagac gttcacgcca gttaatccct caaagttggg
     2041 gggcgtgacc gtaacgccct gccagtggct tgccagcaac tggtgtgatc aggttagctc
     2101 ctcaagttat agcaaccagg atggagcagt ttgggtggcc atcgggaccg ttggcacgcg
     2161 taccgagggt gactgcgatt tttttgacat cctccatgcc cttgctgcat agagtggcgg
     2221 cgcaggctag taaatcgtca tgaatgccat tcatcgaaag acgcttgaac ggtgaaacgt
     2281 atcctgttgg aatcgttccg ttggtgggtt tcattgctga agccattgct tttctccttc
     2341 gagggggtct tgaaaagcgc aatgcagggg ccacgccagg tgttgaactt ggtcccggtg
     2401 gtaagtgaaa aagggattct gcttgcgaga tttgagtgaa aactcagctc gctactctcg
     2461 agcgctgctt acgccgtgat gccttgtctg gcgatttggc acccgaccgt tcgacttggc
     2521 acgcaaatca tagtcggagt catggcgggc aaaacgacta aaaaaccttg gtggcaaacg
     2581 cacctggata taggtcaagt ttttttgagc ggattttcga cgttctattg cccaacaaaa
     2641 atgcgtgctg acccaagaga aatatgcata cctcgaccac gccgcccaca gtacagcatt
     2701 gtctgaagag aagaagtgaa agcataaatg aaaacaccac agttgttaag ctgtggtgtt
     2761 ttgacagttc ctatgctagt tgaaggatgt taaaaatgca aatttctctt aacaatcaac
     2821 cacttacaga gctatcttgg cggaaacgga gggattcgaa ccctcgagga ggctctacac
     2881 cccctactcc cttagcaggg gagcaccttc ggccactcgg tcacgtttcc tgaaaatcag
     2941 agccgcgatt atgtcacgac ttcaccgcat ttgtcagacc ggcggcgtat ccaggccaaa
     3001 cgccttgtgc agcgagcgca cggcgagttc catgtacttt tcgtcaatca cgaccgaggt
     3061 cttgatttca ctcgtcgaga tcatctggat attgatgccc tcctcgctca gtacccggaa
     3121 catggtggcg gccacgccca catggctgcg catgccaatg ccgacaatcg acaccttgca
     3181 gattttggtg tcgcccagca cctccagcgc accaagtttg ggtaccacca cggttttcag
     3241 caactccatg gccttggcgt agtcgccatg attgacggtg aaactgaaat cggtcctgcc
     3301 gtccttgctg acattctgga tgatcacgtc aacctcgatg ttggcatctg ccaccgcacc
     3361 cagaatctgg tacgcaatgc cgggcttgtc aggcacgccc agcacggcga ttttggtttc
     3421 atcgcgggtg aatgcaatgc cagaaacaat ggcttgttcc atgttttcgt cttcctcaaa
     3481 agtgatcagg gtgccagact tggcttcttc aacaatatcg atgtcccacg gggtgaagct
     3541 gctcaaaacg cgcagtttga ccttgtactt gccggcaaac tcgaccgagc gaatctgcag
     3601 caccttggag cccatcgagg ccatttccag catttcttcg aagctgacag tactcaggcg
     3661 gcgcgcctcg ggcaccaccc tcgggtcagt ggtgtaaacg ccatcaacgt cggtgtaaat
     3721 caggcattca tcggccttca tggccgctgc caccgccact gccgaggtgt cagagccgcc
     3781 acggcccagc gtggtgatgt tgccctgctc gtcgaggccc tgaaagccgg tcacgatcac
     3841 cacccggcca gcgtccaggt cagctcgcac tttctgatcg tcaatcgact cgatgcgtgc
     3901 cttggtgtaa gcgctattgg tgcgaatggg cacctgccag cccgcataac tgaccgattc
     3961 gagcccctgc gcctgcagcg cgatggccag caacgcgctt gatgcctgct cgccagtggc
     4021 ggccagcatg tcaagctcac ggttataggc ttcggtcggc ttgccgggtg ccagttcttt
     4081 ggccagggcc agcaggcggt tggtttcgcc actcatggca ctcggtacaa ccaccatctg
     4141 gtggccggcg cgcgcccatt tggcaacgcg tttggcgaca ttggcgatgc gctcggtaga
     4201 gcccatcgat gtgccgccgt atttatgaac aatcaatgac attatggaga tgagctcggc
     4261 gttaaaaccg cgggattgta ccaatgcaat aaggtgccgc gccggatgca aaaaccattg
     4321 gccaccttca gatgcagcgc gtggccgcgg gtttggcaag ctctgatctg gcgcatcagt
     4381 tcgttcaatt gcgcagcgct tggcgccacc tgatgggcac tttgcagcca gtggcgcagc
     4441 acattggtca gccggggccc gctgaacgac tgcagcgcct tgatattggg cggcacaccc
     4501 gcctgcgcca ggtcaagcgc agccagttca agcaacagcg attgagcttg cgccgcatgc
     4561 gccgcgctgc gcgcaaatgt gtcacgaaac tgcgggaaac acgcctgcaa ggcaggcaac
     4621 aggcgtgccc gaatgcggtt gcgggtgaaa cgctcatcca gattggtcgg gtcatcaatg
     4681 aacgccaccg cctgaccagc aagccagcgg cgaatttcgg cgctgctgac ctgcagcagc
     4741 gggcggtgat aacgcagcct gccacgcacc cagcttggcg gcatggcgct caggccgggc
     4801 agacccgacc cccggctcag cgccagcagc agtgtttcta cctgatcgtc ggcgtgttgt
     4861 ccaattgcta ttgattgaat agctggaagc gcttgatccg aaagggctag cgcttcaaat
     4921 gccttgtaac gcgcatgacg tgcagcggcc tccgggcttt gccctgacgc atgtcgggca
     4981 tcgaccttct gcactctcag gggcacccca agaccagcac aaaacgcttt gcagtgctgc
     5041 tcgaagtcgt cggctgcggc ctgcaggcca tgatgcacat gaaacgcctg cacctgacct
     5101 ggccattttt gcacgcaggc cagcaacaag gcggtcgaat ccgcgccacc actgaaagcc
     5161 acggccagcg gcagttccgg cgaaaaagcg gccattgctt gcggcagtga ctgcgtcacg
     5221 gcttggccca ctcagagccg cgagcatcgt caatgtgcac ggcttggcgt gaagataatt
     5281 cgactctgac cccaattaat ctgcttcact tgcctggttt ggtgtcggta aaccggccat
     5341 aactttgcag tcgggcgtag cgccgatcca gcagttcctg ggttttcagg tcgctgacct
     5401 gccgaaacgc atcggtcagc gcacgcttga ggaaagccgc catctgcttg tgatcgcggt
     5461 gtgcgccgcc gaccggctca ttgacgatct tgtcgatcac acccagcgcc ttcagccgat
     5521 gcgcggtaat gcccaatgca tcggccgctt cctgcgcttt gtcggaggtt ttccagagga
     5581 tcgaggcgca cccctcaggg ctgatcaccg agtaaatcga gtactgcagc atcagcacct
     5641 ggtcgcccac cgagatcgcc agcgcaccac ccgagccgcc ttcgccaatg atggtcacga
     5701 tgatcggcac ttcaagctgg gccatctcaa aaatattgcg gccaatcgcc tcggactggc
     5761 cacgttcctc ggcatcaatg ccggggtaag cgcccggtgt atcgacaaag gtgaacaacg
     5821 gcagtttgaa tttttcagcg gttttcatca ggcgcaaggc cttgcggtag ccctcgggct
     5881 tgctcatgcc aaagttgcgc aggccgcgct ctttggtgtc acgccctttt tgctggccga
     5941 tcaccatgca cggcgtgccg ttaaagcgcg ccaggccacc gatgatgctt gggtcgtcgg
     6001 caaaatgccg gtcgccgtgc aactcgataa agtgggtgaa tatctctgac aggtactcca
     6061 gcgtataggg acgctcggtg tggcgggcaa tcttggtgat ctgccagggg ctcaggtcac
     6121 tgtaaatgtc cttggtgagt tgctggcttt ttttggccaa ttggttgatt tcggccgaaa
     6181 tgtccaccgc tgattcagtt tgaacatagc gcaactcttc aatcttgttt tccagatcag
     6241 caatcggggt ctcgaaatcg aggaaggtct tcttagccaa ctaaattctc ctgttcggtt
     6301 gtttccaatc cgctgcggac agtgcaatct cactgcgcgc aagcctgccc tgccgcgcaa
     6361 acacttgatg tgttggggac agggcaatct cgccgcgcgc aagcttggtc tgtcccgcaa
     6421 acacctgatt tgttggggac agagcaatct cactgcgtgt agattggtct gtcccacaaa
     6481 gtctcaggtt gctcaatagg ttaccggcag cgggtccaac gagcgccaaa tataccaagt
     6541 tgcgacggtg cggtacgggg cccaggccag cgccacctcg cgggcgtcgc tgcggctcac
     6601 cacatcgccc gaaaaataac acttgctgat gccgttgatc aggcccacat cgtcaagcgg
     6661 cagtacattc gggcgcatca ggtgaaagat caaaaacatc tctgcggtcc agcggccaat
     6721 gccgcgaatc gtcaccagtt cgtcgatgat ggcctcgtcg tccatgccgg tccattcatc
     6781 gacatgcacc ttgccattgt caaagtgcaa ggccagatcg accaggtatt caaccttgcg
     6841 cacgctcagg ccggctgcgc gcatatcgtc gatcttgagc agcaacactt gcgccggggt
     6901 catctggccg ggcagtttag caaagcggtc ccacacggtt tgcgccgcct tgaccgaaat
     6961 ctgctgaccc accacactgc gcgccagtgt gacaaacgca tcgccccggc tttgcagaca
     7021 ggccttgcca aactgcggga taagccgctt catgacgcgg tctttcttca tcaggtgctt
     7081 gcaagcatcc tgccaatagg ctggcaaagg tgcgcaagac gctatattat caatagctat
     7141 catcgcttgt tcagtatggg ttggaggcta aaaacatgca gaacttcgaa acgatgaagc
     7201 ggtcgcaagc cttgcttgcc gactgggttg tttgcaagcg gcccggcagc gcaagtcgcc
     7261 actgtacagc cgattggctg gcagatttgc aaacttgcgg gttgcaccgc atttcactgc
     7321 agcacctccc agcttgtgcc gctggctgtg tctttcagta ccaccccctg cgccagcaat
     7381 tcctggcgaa tgcgatcggc cagagcgaag tctttggctg ccttggcagc agcgcgcgcg
     7441 gcaatgcgct gtgcaatcgc cgcttcgtca acacctgcac cggcctgcaa aaaggctttt
     7501 ggctcgcctt gcagcaggcc caggcagcca cccagggcct tgagcagacc agccgccgaa
     7561 gacgaacggg tgcggttgac ctcagaggcc aggtcaaaca acacggcaac ggcttctggc
     7621 gtgccaaagt cgttgtccat tgccaccttg aagcgcgccg caaacggctc ggtccagtcg
     7681 atggccaccg cctcgggcgg caccagattg agtgcggtgt acagccgttt gagtgaccca
     7741 cgcgcatcat cgaggtgcac atcgctgtag ttcagggcgg tgcggtaatg ggtgcgaacc
     7801 agaaaatagc gcactgtttc ggggtcgtac ctggtcagca cttcgcggat ggtgaaaaag
     7861 ttgcccagac tcttggacat tttttcgtta tcaacacgca caaacccgtt gtgcacccag
     7921 aatttcgcca gcggctggcc cgatgcgcct tcgctttgtg cgatttcgtt ctcgtggtgc
     7981 ggaaactgca aatcagcgcc accgccatgg atgtcgaagg tttcgccaag cgccgcgcaa
     8041 ctcatggccg agcactcgat gtgccagccg ggacggccaa tggcgtagcc gtaaccctgg
     8101 gcattgccgt cccacttggc atcatctggc tcgctctcct tggcggattt ccacaataca
     8161 aagtccagcg gatcatcctt gccgtccgcc accgccaccc gttcgccagc gcgcagttcg
     8221 tcaagcgact tgcccgacaa cttgccatag ccggggaact tgcgcaccgc aaaattgaca
     8281 tcgccgctgg ccgcctggta ggccaggccg cgcgcttgca gccgactgat cagcttgagc
     8341 atctgcggca catattcggt ggcacgcggc tccagcgtcg gcgcttcaat accaagccgg
     8401 gcgatatccg tatgcatggc gtcaatcatc tcgtcggtca gcgcccggat ggtgatgcca
     8461 cgctcgaccg cgcgtttgat gatcttgtcg tcaatgtcgg tgatgttgcg cacataggtg
     8521 acgcggtagc cgctgctctt caaccagcgc tgcaccacgt caaacgccat catcatgcgg
     8581 gcatggccga tgtggcacag gtcataaatc gtcatgccgc agacatacat gcgcacatgg
     8641 ccggcttgca ggggtgaaaa aggctccaat tcacgcgtca gcgtgttgaa aatagacaaa
     8701 ctcatgggca gtagaactcg gcaaaactta acagacgctg ccatcgacaa gcgccaacag
     8761 gccaatcacc gaaaacccgt cgacgacgca ggcccggcaa tcaaaccccc tcagatacaa
     8821 tgaacttcag tataaagccc cgtctgtccc gactttggcc gctcaactta gccgctatct
     8881 acccaccgac tccatgaagc aaacccgttc gctcgtcact tgcgccctgc gtctgctcac
     8941 gctggtggcg gccatgaccg ttttttccgg ccatgccgac gaagtggccg atgtcgcaca
     9001 gttgctgcgc agcaagcagt ttgcatcggc cctgacgctg gctgatcgct acctggccga
     9061 caagccagcc gacccgcaga tgcgcttttt caaaggggtg atccagcgca acctcgggca
     9121 gcaagccgag gcaatcgtca ccttcaccca gctcaccgaa gattaccccg aactgcccga
     9181 gccgcataac aacctggccg cgctgtatgc cgagacggga caaattgaca aggcccgcct
     9241 ggcgcttgag atggcggttc gcaccaaccc caactatgca attgcccagg aaaatctggg
     9301 cgatatctat gcccgcctgg ccagtcaggc ctatgccaag gccggtcagc ttgaccgcag
     9361 taaccccgct gtgccacgca agctggcgtt gatcggcgag attttcaaac ccgcattgcc
     9421 aggttcgcga cctgttgcca gcgcagcagc ggcagccggg tcagcgccga tgcctgctgc
     9481 gcctgccagt cagcctgcca ctgcaacagt caccccgact ggcaatccgg cagtcaggta
     9541 gccagtcgga cagatggacc gaacagcgcg attcgccccg agcgatgggt gccggatttg
     9601 tcggcccata caaggccggt gcaaaatggc ccgctgtcag ttcgcggccg atgcgccaga
     9661 tggccgccag acacccaggt ggcgcgcccc gtcagttgcg gcatcgccag acccacgaca
     9721 acttggcggc gcggcgacaa tacttggctg gcaaagccag gtcaatgcca atcttcatca
     9781 aggaatcatg tcatgaaatt caggtctttt agcacgctag cccttactgc gcttgcgtca
     9841 ctagctatat tttcaatagt tagcccagca agcgcgcaag ccgcaccgca ggtcaagttt
     9901 gccacttctg cgggtgattt tgtggtcgag gtgtaccccg acaaggcccc aaaaacggtc
     9961 gagaactttc ttcagtatgt caaggacaag cactacgacg gcaccatttt tcaccgtgtc
    10021 atcccgaact tcatgatcca gggtggcggc tttgatgcca agtacctgca gaaaccgacc
    10081 cgcgcaccgg tgccacacga aggccgcgag gcgcttgaaa aaggtggccc gcgcaatgtg
    10141 gtcggcaccg tggcaatggc gcgcaccaat gacccgcagt cggctaccgc acagtttttc
    10201 atcaacgtca aggacaatgg ctttctggac cccagcgccc agtcgtttgg ttataccgtc
    10261 tttggcaagg tcatcaccgg cttcgacgtg gtcgaaaaaa tcaagctcac accaaccggt
    10321 gccggtggtc cgtttgccac cgatgttccc aaaaccccca ttcttatcaa cactgcaacc
    10381 ctggtgaaat aaatcatgag caacccgcaa gtcgaacttc acattgccaa ctacggcacc
    10441 atcacccttg agctggacca ggacaaggca cccaacacgg tgaccaattt tctgagttac
    10501 gtgagcaagg gccactacga caacaccatt tttcaccgtg tcatccccgg cttcatggtg
    10561 caaggcggcg gcatggagcc cggcatgaag gaaaaaaagg gtggccaacc gatccagaac
    10621 gaagccaaca acggcctgaa gaaccagagc tacacggtgg ccatggcccg cactggcgac
    10681 ccgcattcag ccaccgcaca gttcttcatc aacgtggctg acaatggctt tctgaaccat
    10741 accgcaccga gtgcgcaagg gtggggctat gcagtgtttg gcaaggtcgt ggcgggcgcc
    10801 gaggtggtgg acaagatcaa ggcggtgaaa actgccaaaa agggctttca tgacgacgtg
    10861 ccggtcgaag atgtggtgat tgaaaaggct gtcgcgctgt agcctggccc caagccatga
    10921 acacgaccgc gcaccctccc cttgccaatg gtcgttcgcg gccggtctgg ccgcagcttg
    10981 cagcaccccg gcggtggcag tcggttgatg tcatctccga cctgcacctg cacgcgggtg
    11041 acatgccgac cttctcggca tggcagaact acatggcaac aaccccggcc gatgcggtgt
    11101 tcattctggg cgatctgttt gaggtgtggg tgggtgacga tgtgcttgcg gccccaagcg
    11161 ggcgatcgct tggttttgaa gcgcactgcc agcaggttct ggccagcgcg gcgcagcgac
    11221 tgagcctgta tttcatgcac ggcaaccggg attttctgct cggcgatggg tatgcgcacg
    11281 gtgcttgcat gaccttgctg tcagacccga cggtgctgac gttcgacgcg caaggctggc
    11341 tgctgaccca tggtgatgcg ctgtgcctgg ccgatgccga ctatcagcag tttcgtgcca
    11401 ccgtgcgcac ggccgcctgg cgtgatgaat tcctggccag gccgctgacc gagcgccacc
    11461 ggattgcccg tagcctgcgc actcaaagcg agtcgatcaa ggccgccggg gcgccggttg
    11521 ccgagattga ccgagccagc gcctgcgact ggctgcgagc cgccggggct ggcaatctgg
    11581 tccacggcca cacccaccgg cccgacgatg agcagctcga tgacgcattg gctgacacat
    11641 cggcgccact gcatcgatgg gcgctaagcg actgggatgc caatgcccgt ccgccacggc
    11701 tgcaagtgct gcgcctgagc gtggcgcagc aaccccgggg catcacgctg ccatcagggt
    11761 tttagcagca acttcaacac gttttgcaga cggcgcgact ggctcgcgtc aaatgcgctg
    11821 cccaatacgc tggccgcatc ctgcccccag gtttgcgccg gtggcgggtc gttgcggcgg
    11881 gtcagcaatt gcaactggag catgcgccgg tcactcaggt actcggctgg tgttggcact
    11941 tcagccgcca tttcgagccg cagcagcgcc gtgctggcag cctctgtcgg ggtctgaccc
    12001 aaggccttga cccaggccgt acgcaccgct ggcgagaccc gattgcccag ctcctgcacg
    12061 ctgggcacca gggctgcgtc acgctgctcc caggcctgca gcaaatgcgt cagtgcttcg
    12121 ccgtgggctt gggcagacag gcggcgcagc gccatttgcg cctgttccag cgcatcgcgt
    12181 tgcgccctga atgctgcatc gcccagtcgc ggcgcgtcaa agcgctcgcc catcggtgcg
    12241 cggtcagcgc gctcaggtcg gctgccacgc tcgtcggctc gaccagggcc gcgtgtaccg
    12301 acatcacgtc caccggtccg ggggccgtcc ttgcggtcac caaacttgcc accacgtccg
    12361 gccatggcag gcgcttcttt tcgcatgcca ggtcggtcgt cgccgcgcat cgccaccacc
    12421 ggctttctga ccactgtggg cttgggtggc tcggcggcgg gttctgcagt agccgccgca
    12481 tcagcggcat cggcagccgc gtcagcggca cccaagggcg cttcagcagc tacactttca
    12541 gtagcatctt gcgcagacgg agactgggct ggcggcacat tttcatcgga tttttcagcc
    12601 gtggcggcga ccgctgcctg ggcttgcgcc tggccacgca aggcggcatc cagcaccgcc
    12661 atggcactgc ggatggcctg gccatcgccc g
//
LOCUS       DAOSLL010000049        12645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5019_length_12645_cov_5.590627, whole genome shotgun sequence.
ACCESSION   DAOSLL010000049 DAOSLL010000000
VERSION     DAOSLL010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 12645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12645
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5019_length_12645_cov_5.590627"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..248
                     /locus_tag="PLB25_03495"
     CDS             9..248
                     /locus_tag="PLB25_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase N-terminal SAM-like
                     domain-containing protein"
                     /protein_id="HOE40685.1"
                     /translation="MQSTRLLDQFRERIRYMHYSLSTEKAYLRWVRFILRWHGRSGQM
                     QHPRPMGAPDIEAFFTLLASAPRKRCWVTQMSAPP"
     gene            complement(465..2723)
                     /locus_tag="PLB25_03500"
     CDS             complement(465..2723)
                     /locus_tag="PLB25_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOE40686.1"
                     /translation="MNSASSPERLQAWRKQLDCGFAQLDDVFADCMAEASAQLSPDGL
                     DDYLAQARFLGKMGRGVEPVLIFLQEWPSVANMVGEDALSPVMTAVKLINKSPNSKAI
                     APLLQTLAAVARRLPTQSQLQHYLTLSLNLMERTSSSIHGIHKSYPSPSLPTYFEKSA
                     QLLELLSLQGLSNWVDYGIRNYTHHPEQQRDYFSMASSDSRAVLQRERHGTLLMDHTR
                     ELDLYLQALWQDGDLLVPYSTTFDVVHQPMPYFDAMGIRLPDVYDQRAGIAGIDRYRA
                     ALAHMAAHRRWSTPIFADNFSPAQRLAIECFEDSRVDALTLREYPGLRRVLQALHPAP
                     LDGACNPATHSCLRHRLTVLSRALLEPPKRFEAPQLQDWLQRFQALLACGESSTAEMA
                     GLALSYLGKTRLQSDQLPDTWFTDTVVDYRDDNRHLWRFHELSDDEESFDNPAQVPPA
                     QEIQALPPRHYPEWDYSSQTFRPDWVSLYERLHPSGQAADIDALLAKHSALAKQLKRL
                     LDLLKPQDKLRLRFQEDGSELDLDVAIRSLIDLKSGSQPDPRINMSHTTDGRSIAVTL
                     LLDLSASLNDKARGSEQTVLQLSQEAVSLLAWAVQQLGDPLAIAGFHSNTRHDVRYLH
                     LKGFGESWGDAVKARLAAMQAAYSTRMGAALRHAARTLAKQPADKKLLLVLTDGQPAD
                     IDVKDERLLIEDAKEAVRELDHQGIFTYCINLDAAADAYVADIFGKRYTVIDNICRLP
                     EQLPKLFMALTR"
     gene            complement(2763..3572)
                     /locus_tag="PLB25_03505"
     CDS             complement(2763..3572)
                     /locus_tag="PLB25_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbQ/NirQ/NorQ/GpvN family protein"
                     /protein_id="HOE40687.1"
                     /translation="MTNLAQQYSITQEPFYQPQSNEVALYTAAYQARLPVMVKGPTGC
                     GKSRFIEFMAWKLGKPLITVACNEDMTASDLVGRYLLEANGTRWLDGPLATAARIGAI
                     CYLDEIVEARQDTTVVIHPLTDHRRQLPLDKKGELIHAHADFQLVISYNPGYQSLMKD
                     LKQSTKQRFVGFDFDYPTAGLEADIIAKEAGIGLADASRLVKIGAAARALKGHGLDEG
                     ISTRLLVYAATLIKGDVAPVDACRMALVRPITDDADIRATLDHAIDAVFGD"
     gene            complement(4006..5385)
                     /locus_tag="PLB25_03510"
     CDS             complement(4006..5385)
                     /locus_tag="PLB25_03510"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011313150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase"
                     /protein_id="HOE40688.1"
                     /translation="MDQSNRYADLSLKEEDLIAGGKHILVAYKMAPKPGYGYLEAAAH
                     FAAESSTGTNVEVSTTDDFTRGVDALVYYIDEATEDMRIAYPIDLFDRNMTDGRMMIV
                     SFLTLVIGNNQGMGDIKHAKIHDFYMPERAIQLFDGPSKDISDMWRILGRPVKDGGYI
                     AGTIIKPKLGLRPEPFAKAAYQFWLGGDFIKNDEPQGNQVFCPAKKVYPLVYDALKRA
                     QDETGQAKLFSANITADDHYEMCARADFILEAFGPDADKVAFLVDGFVGGPGMITTAR
                     RQYPNQYLHYHRAGHGMITSPSAKRGYTAFVLAKISRLQGASGIHVGTMGYGKMEGEG
                     DDRNIAYMIERDECQGPVYFQKWYGMKPTTPIISGGMNALRLPGFFQNLGHGNVINTA
                     GGGSYGHIDSPAAGAISLRQSYECWKAGADPIEFAKEHKEFARAFESFPKDADQIFPG
                     WREKLGVHK"
     gene            5584..6354
                     /locus_tag="PLB25_03515"
     CDS             5584..6354
                     /locus_tag="PLB25_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOE40689.1"
                     /translation="MPIVVFNQKGGVGKSTITCNLAAISASRGLRTLVIDLDSQGNST
                     SYLLGSSAKDDQPTVAGFFEHALSYSFKNLPPTDFVVSTPFEKLDVMPSSPGLNELQV
                     KLESRQKIYKLREGLKQLAETYDRIYIDTPPALNFFTRAALIGANGCLIPFDCDSFSR
                     QALYSLLENVQEIRADHNSGLQVEGIVVNQFQARANLPQRMVAELLAEGLPVLQPYLP
                     SSVKIRESHEQCLPMIYLDPTHKITLALVALHDGLRTQ"
     gene            complement(6632..7294)
                     /locus_tag="PLB25_03520"
     CDS             complement(6632..7294)
                     /locus_tag="PLB25_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010926277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HOE40690.1"
                     /translation="MCSHYQGIKERERYRRHFGVEPPADLGRHDLWPGYTGTFIRANP
                     QAADMAAPEIEARNGLFGLVPHWSADTRITKATYNARSETVAGKPSFREAWRKAQHCI
                     IPADAFFEPDWRSGRAVPTCIARADQAPMGLAGLWSAWRSPQGELLYSYTMLTINAAD
                     HPLLQLMHKPQDEKRMVVILPENRYTGWLRADASHSGQFLQQFPAALLQASAPRVAGG
                     LF"
     gene            7333..7866
                     /gene="thpR"
                     /locus_tag="PLB25_03525"
     CDS             7333..7866
                     /gene="thpR"
                     /locus_tag="PLB25_03525"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HOE40691.1"
                     /translation="MMESATPQVNPGQRLFLALWPNDAVRQQLSVHAQHWTWPTGCVR
                     YLSADWHVTLHYIGKVATDQIPDLAAHVDMAVQPFELRLDQPELWPHGLAVLVASEVP
                     APLHALRQRLGEAVAGFGLPVEARPFRPHVTLARRAKEAIAPLMSAPVRWRANCYGLV
                     ESTGSSDGRYRIIRQYG"
     gene            complement(8008..8883)
                     /locus_tag="PLB25_03530"
     CDS             complement(8008..8883)
                     /locus_tag="PLB25_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40692.1"
                     /translation="MQLDFFNDSLNVSLRNDVIFALEQCDVPLAQQAWEKLGQHCPDD
                     TRQVALLLLLIVFLAARSQVPFQSHADLSAARLEMQNKITPAAQDSLGSAAAAVWLRE
                     RWQELARRAALLPFRAEHADDHAATLWLHTANWQMAADAVAQIESWRRIPAPLSWMLQ
                     ARLRLQGLQANWGLLAELAWLSPNRLGNVVRQAADPILQQWVTKFEQGFEDAGDASDL
                     VWLPAWLLTQRPALAPHLAQAQASQHSEPEQAMRVLLELLGLERQGRQREVIEHRKTL
                     RGLHSGLYAAYLATR"
     gene            9801..>12645
                     /locus_tag="PLB25_03535"
     CDS             9801..>12645
                     /locus_tag="PLB25_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE40693.1"
                     /translation="MATTDPEPLSSQATAQLLNELQVHQIELEMQNDELRTAQQSLEA
                     SQAFYFDFYDLAPVGYCSVGEDALIRQSNLTTANLLGLPRNQLTHQPFSKFIFKDDQA
                     LFYRYRHKLLDTRQSQSCELRLLRRNSTPFWVNLATIVASDETGVPLLRMVISDITER
                     KRAEDALKQSEERWQFALESGGDGLWDWNLQTGEAFFSPRYKQMLGYAEHEFENNAEA
                     WSKSIHPDDAPGVMTTLRPYLDGKDGTASVEFRMLCKDGSWLWTLGRGKVIGRDASGK
                     ATRMIGTNADITERKQAEKVIRESESRFRSLMENIAGVAVQGCALDGTVLYWNPASER
                     LYGYSATEVLGANLLDLIFPPDMHEGFKLAMQQMATTGEPIPADELMLKNKAGTPTPV
                     FSSHALVQPIGREPELFCLSIDLSERNKARDALQQSEAFKEAILNSVAAEIVVIDGNG
                     VILAVNQHWRQYALDNGLEPGQVAPNTGVGSNYLSICAMGAQADDNARQACDGIWAVL
                     QGRLPRFSLEYPCHSPGRLRWFNMVVMPLGYNGQGASITHTDVTAIKQADERVLSSER
                     HLRAIFETSLDAVIGMDDQGRITDWNQQAQTIFGWHKDEVMGLMLHDTIAPAHYRAAH
                     HQGLTRFLVTGQSDMLGRRLELVGQRRNGEEFPITLSVLPFKTGEGYRFTAFVDDISE
                     RKRVTAALQESEQKFRLIAENTSDGITIFDKNGHVQYVSPSVMRQLGSSEQEELARTN
                     AEVFSIMHPDDRDEVFARIDEAIKNIKDSLTYSYRVKHQSGHYFWREDNVSLIFDSNQ
                     EYGGAYVISRDITERKRLEEQVRQLAYFDPLTKLPNRRMLGDRLSQTMALSKRSGRYA
                     AVMVLDLDNFKPLNDKHGHLVGDMLLVEVASRLLHCVRAVDTVARFGGDEFVVVLGEL
                     DVDSAESRAQAQVIAEKILASLSAPYVMTRRKDE"
BASE COUNT         2572 a         3605 c         3799 g         2669 t
ORIGIN      
        1 caccaccttt gcaatcaacc cgcctgctcg atcagttccg cgagcgcatc cggtacatgc
       61 actatagcct gagcactgag aaggcttatt tgcgctgggt gcggtttatt ctccgctggc
      121 acgggcgcag cggccaaatg caacatcccc ggcccatggg cgcgcctgac attgaggcct
      181 tcttcaccct gctggcatcc gcaccgcgca agcgttgctg ggtcactcag atgtcagcac
      241 caccatgatc gacaccgatg tgctcaaggt gacagccggt ggcacggtcg gccctttgga
      301 ttcattggct ggcctggctg tttgaaggtc cacaagtgct gcattgcagt cttcgtccga
      361 tcgccaactc tggcatgcaa atcatcaaca aaaagtggtt ccagcccaat tgcagcaagc
      421 gctggcagct atataaaaaa tagtagttgg cccccgccaa aacctcaccg cgtcagcgcc
      481 atgaacagtt ttggcaactg ctccggcaag cggcagatgt tgtcaatcac cgtgtagcgc
      541 ttgccaaaga tgtcggccac ataggcatcg gcggcggcgt ccaggttgat gcaataggtg
      601 aagatgcctt ggtggtccag ctcgcgcacg gcttccttgg cgtcttcgat cagcaggcgc
      661 tcatctttca cgtcaatgtc ggcgggttgg ccgtcggtca gtaccagcag cagttttttg
      721 tcggcgggct gcttggccag tgtgcgcgcg gcgtggcgca gcgctgcacc catgcgcgtg
      781 ctgtaggcgg cctgcatggc ggccaggcgg gctttcaccg catcgcccca gctctcgcca
      841 aagcccttca ggtgcaggta acgcacatcg tggcgggtgt tggagtgaaa cccggcaatc
      901 gccagcgggt cgcccagttg ttgcaccgcc caggccagca atgagactgc ctcctggctc
      961 agttgcagca cggtttgctc cgagccacgt gctttgtcat tgagcgaggc tgacaagtcc
     1021 agcagcagcg tgacggcgat gctgcggccg tcggtggtgt ggctcatgtt gatgcgcggg
     1081 tcgggctggc tgccgctttt caggtcaatc aacgagcgga tcgccacgtc cagatccagc
     1141 tcgctgccgt cttcctgaaa acgcaggcgc agcttgtctt gcggcttgag caggtccagc
     1201 agacgcttga gttgcttggc cagcgcgctg tgtttggcca gcagcgcgtc gatgtcggcg
     1261 gcctggcctg acgggtgcag gcgctcgtac aaactgaccc agtccgggcg aaaggtctgg
     1321 ctgctgtagt cccattccgg gtaatggcgt ggcggcaggg cctggatttc ttgagcgggt
     1381 ggcacttgag ccgggttgtc aaacgactcc tcgtcatcgc tgagctcatg aaagcgccac
     1441 aggtgccggt tgtcgtcgcg gtagtccacc accgtgtcgg taaaccaggt gtcgggcaac
     1501 tggtcgcttt gcagccgggt tttgcccaga tagctcaggg ccagcccggc catctcggcg
     1561 gtgctggatt cgccgcatgc cagcagcgcc tgaaatcgtt gcagccagtc ttgcagttgc
     1621 ggcgcttcga agcgcttggg cggctccagc agcgcgcgcg acagcactgt caggcggtgg
     1681 cgcaagcagg aatgtgtagc agggttgcaa gcgccatcaa gcggtgccgg gtgaagtgcc
     1741 tgcaagacgc ggcgcaggcc ggggtattcc cgcagcgtca gcgcatcaac ccgcgagtct
     1801 tcaaaacact cgattgccag ccgctgcgcc gggctgaagt tgtcggcaaa aatgggcgtg
     1861 ctccagcgcc gatgtgccgc catgtgggcc agcgcggcgc ggtagcggtc gatgcccgcg
     1921 atgcccgcgc gttggtcata cacgtcgggc aggcgaatgc ccatggcatc aaagtagggc
     1981 atgggctggt gcacaacgtc aaatgtggtg gagtagggca ccagcagatc accgtcttgc
     2041 cacagtgctt gcaggtacaa atccagctct ctggtgtgat ccatcagcag cgtgccatgg
     2101 cgttcgcgct gcagcacggc ccggctgtcg ctggaggcca tgctgaagta gtcgcgctgc
     2161 tgctcggggt ggtgggtgta gttgcgaatg ccgtagtcca cccagttgct caaaccttgc
     2221 aggctcagca gttccagcag ttgggccgac ttttcgaaat aggtaggcag gctcgggctg
     2281 gggtagcttt tgtgaatgcc atgaatcgag ctgctggtgc gctccatcag gttcaggctc
     2341 agggtcaggt agtgctgcaa ctgcgactgg gttggcagcc gccgcgccac cgccgccagg
     2401 gtctgcaaaa gcggcgcaat cgctttgctg ttgggcgact tgttgatcag cttgacagcc
     2461 gtcatcaccg ggctcagggc atcttcaccc accatgtttg caacgcttgg ccactcctgc
     2521 aaaaagatca gcaccggctc aacaccgcgc cccatcttgc ccaaaaagcg cgcttgtgcc
     2581 aggtagtcgt ccagtccgtc ggggctgagc tgggcgctgg cttcggccat gcagtcggca
     2641 aatacatcgt caagctgggc aaagccgcag tccaactgct tgcgccaggc ttgcagccgc
     2701 tcggggctgg aggctgagtt catggcgggt ttgactgcgt tggccgcgac caataaagcg
     2761 cgctaatcac caaacaccgc gtcaatcgca tggtccagtg tggcgcggat gtcggcatcg
     2821 tcggtaattg gccgcaccag cgccatgcgg caggcatcaa ccggagccac atcgcccttg
     2881 atcagggtgg cggcatagac caatagccgg gttgaaatgc cttcatcgag cccgtggcct
     2941 ttgagtgccc gtgccgcagc gccaattttg accagacgcg aagcgtcagc caggccaatg
     3001 ccggcctctt tcgcgatgat gtcggcttcg agcccggctg tcgggtaatc aaagtcaaag
     3061 ccgacaaagc gctgcttggt gctttgtttc aggtctttca tcaggctctg gtagccgggg
     3121 ttgtaggaga tgaccaactg aaaatcagcg tgcgcgtgaa tcagctcgcc ttttttgtcc
     3181 agcggcaact ggcggcggtg gtcggtcagc gggtggatga ccaccgtggt gtcttgccgc
     3241 gcttcgacga tttcgtccag ataacaaatg gcaccaatgc gggcggcggt ggccagcggg
     3301 ccgtcgagcc agcgggtgcc gttggcctcc agcaggtagc ggcccaccag gtctgacgcg
     3361 gtcatgtctt cgttgcaggc cacggtgatc agtggcttgc ccagtttcca tgccatgaat
     3421 tcgatgaagc gcgatttgcc gcagccggtg gggcctttga ccatgaccgg cagccgcgcc
     3481 tggtaagccg ccgtgtacaa ggcaacctcg ttgctttgcg gttggtaaaa cggctcttgg
     3541 gtgatgctgt attgctgggc aagattcgtc atggcggatt cctgaattgg caagatcttg
     3601 gtatcggggc ggtgggggca aataccttgg taactgtaac ccgcagcgcg cattgggtgt
     3661 ccgactccgc gaatgtcccc cggttcgggc agagcccgaa cctcctcctt gatttggcaa
     3721 aaccccaagt ccacgctacg tcagacaccc aacgcacgct gctagtcgct accggtaact
     3781 acttcgttgt gggcaattta ttgattcgct tgcgacgtta gcggtcggct tgttaagcac
     3841 taagcgtctg gtgatgggtt gccttgcaga gcaaaatcaa ggaggaggca cgggcgtagc
     3901 ccgggccggg ggacatttgc ggcgcagggc aacccgtcgc caggcgcgtt tcaccagaga
     3961 aaccagaaag aaatctccca gtctctctgc aacattaagc gttgcttact tgtgaacgcc
     4021 aagcttctcg cgccagcccg ggaaaatctg gtcagcatct ttcgggaacg actcgaacgc
     4081 acgagcaaac tctttgtgct ctttggcgaa ctcgatcggg tcggcaccgg ctttccagca
     4141 ctcatacgac tggcgcagcg aaattgcacc ggctgcgggt gagtcaatgt ggccatagga
     4201 gccgccaccg gcagtgttga tcacgttgcc gtggcccaga ttctggaaga agccaggcag
     4261 acgcagcgca ttcatgccgc ccgagatgat cggggtggtg ggcttcatgc cataccattt
     4321 ctggaaatag accgggcctt ggcactcgtc acgctcaatc atgtaggcga tgttgcggtc
     4381 gtcgccttca ccttccatct tgccgtagcc catggtgccg acgtggatgc ctgatgctcc
     4441 ttgcagacga gagattttgg ccagcacaaa agcggtgtaa ccacgcttgg cgctggggct
     4501 ggtgatcatg ccgtgaccgg cgcggtggta gtgcaggtac tggttggggt actggcggcg
     4561 ggcggtggtg atcatgccgg ggccgccgac aaaaccgtcc accaggaagg cgaccttgtc
     4621 tgcatccggg ccaaaggctt ccagaatgaa gtcagcgcgg gcgcacattt cgtagtggtc
     4681 gtcagcagtg atgttggccg agaacagctt ggcctgaccg gtttcgtcct gggcacgctt
     4741 gagggcatca tagaccagcg gataaacctt cttggccggg cagaacactt ggttgccttg
     4801 tggctcgtcg ttcttgatga agtcgccacc cagccagaac tggtaggcag ccttggcaaa
     4861 cggttcgggg cgcaggccca attttggttt gatgatggtg ccggcaatgt agccgccgtc
     4921 tttcaccggg cgacccagaa tgcgccacat gtcagagatg tctttggacg ggccgtcaaa
     4981 cagttggatg gcgcgctcag gcatgtaaaa gtcgtggatc ttggcgtgct tgatgtcgcc
     5041 catgccctgg ttgttgccga tgaccagcgt caggaacgac acaatcatca tgcggccgtc
     5101 ggtcatgttg cggtcaaaca agtcaatcgg gtacgcaatg cgcatgtctt ccgtggcttc
     5161 gtcgatgtaa tagaccagcg catccacacc gcgggtgaag tcgtcggtgg tactgacttc
     5221 cacattggtg ccggtggagg actcggcggc aaagtgcgct gccgcttcca ggtagccgta
     5281 gccgggcttg ggggccatct tataggccac cagaatgtgt ttgccaccgg caatcaggtc
     5341 ttcttccttg aggctcaggt cagcgtaacg gttcgattga tccatgggtt atctcctgtt
     5401 agatgggatt gggctgtcga tggggtcaac tatagacagg cttattcatc atgaaaattc
     5461 aaataaattg ccaatcaaat taggtttaac tgatgagttg cacgtcgaga ctggctggca
     5521 aagtcaagga caatgacgct tcaacctgcg tttgttcttt tgccaaaaat aaaggagtca
     5581 cttatgccaa ttgttgtatt caatcaaaag gggggcgttg gcaagtccac catcacttgc
     5641 aatctggccg ccatcagtgc cagccggggt ctgcgcaccc tggtgattga cctggattct
     5701 caaggcaact ccaccagcta cctgctgggc agcagcgcca aagacgacca gcccacggtg
     5761 gcgggctttt ttgaacatgc gctgagctac agcttcaaga acctgccgcc caccgatttt
     5821 gtggtctcga caccgtttga aaaactcgac gtgatgccgt caagcccggg cttgaatgaa
     5881 ctgcaggtca agcttgaatc gcgccagaag atctacaagc tgcgcgaagg cctcaagcaa
     5941 ttggctgaaa cttatgaccg catttacatt gacacgccgc ctgcgctgaa cttctttacc
     6001 cgcgctgcgc tgattggtgc caatggctgc ctgataccgt ttgactgcga cagtttttcg
     6061 cgccaggcgc tgtattcgct gctggagaac gtgcaggaaa ttcgtgccga ccacaactcc
     6121 gggctgcagg tcgaaggcat tgtggtcaac cagtttcaag cccgtgccaa cctgcctcag
     6181 cgcatggtgg ccgaactgct tgcagagggc ctgccggtgc tgcagcccta tttgccctca
     6241 tcggtgaaga ttcgcgagtc gcacgagcag tgcctgccga tgatttacct tgaccccacc
     6301 cacaagatca cactggcgct ggttgcattg catgatggct tgcgcacgca atagccctga
     6361 ttgtcccttc agcagcccgc cacaccggtt gtgtgtcgct cgatcagcga ggccaacgga
     6421 tcaccaataa gaccgctgct ggaaccgggt cggcccaggg cgggaatcag gaaattcggc
     6481 tggggcatag ggaaaatgtc cgacacgggc gaatgcgagc tgaccatcac ttgctgcgca
     6541 ctttcttcag ttgccacctt gagaccgcag tcggccttta aaaacctctc cgaaatgcat
     6601 catctgccgt cccccttggc aacaaccggc atcaaaacaa gccaccggcc acccgcggcg
     6661 cgctagcctg caacaacgcc gctggaaact gttgcaggaa ttgcccgctg tggctggcat
     6721 cagcgcgtag ccagccggtg tagcggtttt ccggcaggat caccaccatg cgcttttcat
     6781 cctggggttt gtgcatcaat tgcagcagcg gatggtcggc cgcgttgatt gtcagcatgg
     6841 tgtagctgta cagcagttca ccctgcgggg agcgccaggc tgaccagagc ccggccaacc
     6901 ccatcggcgc ctggtctgcg cgggctatgc aggtcggcac cgctcggccg ctgcgccagt
     6961 ccggctcaaa gaaggcatcg gccgggatga tgcagtgttg tgccttgcgc caggcttcgc
     7021 gaaagctcgg tttgccagcc acagtttcgc tgcgggcgtt gtaggtggct ttggtgattc
     7081 tggtgtctgc cgaccagtgt ggcaccaggc caaacagacc gttgcgtgcc tcgatttccg
     7141 gggccgccat atcagccgcc tgcgggttcg cgcgaataaa ggtgccggtg tagcctggcc
     7201 acaggtcgtg cctgcccaag tcggcaggcg gctccacacc gaagtgtcgg cggtaacgct
     7261 cgcgctcctt gatgccttga taatgggaac acatggtctg atactgtcgt gtttttggac
     7321 tttcaatgcg tcatgatgga atcagcgaca ccccaagtca accccggcca gcgacttttt
     7381 ctggcgctgt ggccaaacga tgctgtccga cagcaactga gtgtccatgc gcaacactgg
     7441 acttggccga ccggttgtgt gcggtacctg tctgccgact ggcatgtcac gctgcattac
     7501 attggcaagg tggcgaccga ccagattcct gaccttgctg cccatgttga catggccgtc
     7561 cagccgttcg agctgcggtt ggatcagccc gaactctggc cccacggatt ggccgtgctg
     7621 gtcgccagcg aggtgccagc accgctgcat gcgctgcggc agcgtcttgg ggaagcggtg
     7681 gccgggtttg gtctgccagt cgaggctcgc ccatttcggc cccatgtcac cctggcgcgc
     7741 cgtgcaaaag aggccatcgc gcctttaatg tcggcaccgg tgcggtggcg ggcaaactgt
     7801 tatgggcttg tcgaatcaac gggaagcagc gatggacgct accggatcat tcgccagtat
     7861 gggtaacggc agaacgggat ttgttgcgca caccccagac ccatagcctg gaagaggtca
     7921 aaaagccggt gcaaaagggg ccgctgcggt cggtacccag ccgatgcgcc ataagtccga
     7981 caggttcctg gggctggctt ctgcctttca acgggtcgcc agatacgccg catacaggcc
     8041 gctatgcagg ccgcgcaggg tcttgcggtg ttcgatgacc tcacgttggc ggccctgccg
     8101 ctcaagtccg agcaactcaa gcagtacccg catggcctgc tctggctcgc tgtgctgcga
     8161 ggcttgcgcc tgcgccaaat ggggtgccag tgcaggccgc tgggttagca gccaggccgg
     8221 cagccagacc aggtcgctgg catcgcccgc gtcttcaaag ccttgttcaa atttggtcac
     8281 ccactgctgc aggattgggt cagcggcttg gcgcaccacg ttaccgagcc gattcggtga
     8341 cagccaggca agttctgcca ataagcccca gttggcttgc aagccttgca gccgcaaacg
     8401 ggcttgcagc atccacgaca agggtgccgg aatgcgccgc caggattcga tttgcgccac
     8461 cgcatcagcc gccatctgcc agtttgcggt gtgcagccaa agcgttgcgg cgtgatcatc
     8521 ggcgtgctct gcgcgaaatg gcagcaaagc agcgcgcctg gccagttcct gccagcgttc
     8581 gcgcagccac acggcggcag ccgcactgcc caaactgtcc tgcgctgcgg gtgtgatctt
     8641 gttttgcatt tccagccgtg cagcggacag atcggcgtga ctttgaaacg gcacttgcga
     8701 gcgagcggcc aggaacacaa tcagcagcag caataaggca acctgacgag tgtcgtcggg
     8761 gcaatgctgc cccagttttt cccaggcctg ttgcgcaagc ggcacgtcgc attgttccag
     8821 cgcaaatatg acatcgttgc gcaggctgac gtttaggcta tcgttgaaaa aatcgagctg
     8881 catgaatggc aattttcggt tacgttgcca agtagccatt gatcatccca accccgcacg
     8941 cggggcaggc gttctcaaag gaacatgggc taggccgacg gcgcaagctg ctagggtttc
     9001 atctgccaaa tcggcatcca gcccttatgc agactgcgtt gccagctata tttttgattg
     9061 ttaacatgat atctgctcct taggggcgcc tgacagaaag cccacccaaa acggcatcac
     9121 agatcgccca atattgtttc agcatacagg gttaccttag gcggtttttt tagccatgac
     9181 cagccggttg tttttacgtt gatcatcacg gcggggtttg gtcaactttt gtccgaactg
     9241 ataccttgag tggcctgttt tgtcatcaat ctgaggatgc cttgctgtga aagccctcgt
     9301 caaagctgat cacggccaga ctggcgaaac gcttttggcc tgtgtcagtc gccaaatcaa
     9361 gagcggagaa tccggtggat caaccgcaag aagggagcgg ggaactgttc gtcataatgg
     9421 tcatgttgtg caatgcgcgc tatcgttacc atccatgcgt cggcttgtgt cgcacaaatc
     9481 acccatcttt gggcttgaaa acatattggt gacggatgcc gataaccaca aaaatttact
     9541 cgttgggctg gcagtcatgc aaggacaccc tcgaaacggg gcgacagttc ctgggcattg
     9601 cgccacacat tttttccatc cgttaagcct gatttgatca attgcctggg aagactagag
     9661 tagaacgaga gataagagca agaatcataa ggatagccat ggatcgcgaa aatgaccacc
     9721 ccgccccagc acaagtttgg cttgattcag cagagttatt gcgggtgagc actgaagcgc
     9781 tttttcggct acgcgccacc ttggcgacca cagatcccga gccactgtca tcccaagcca
     9841 ctgctcaatt gctgaatgag ttgcaggtgc atcagattga gctggaaatg cagaacgacg
     9901 agttgcgaac cgcgcaacag tcgctcgaag catcacaagc cttctatttt gatttttatg
     9961 acctggcacc agttggctac tgttccgttg gcgaagacgc cctgatcagg cagtccaacc
    10021 tgaccactgc gaacctactg ggcctgcccc ggaatcaact gacccatcag cccttcagca
    10081 agttcatttt caaagacgac caggcgctgt tttaccggta ccggcacaag ttgctcgata
    10141 cccgtcaatc gcaatcatgc gaattgcgct tgctcaggcg taacagcacg ccgttctggg
    10201 taaatctggc aacaatcgtg gcatcggatg aaaccggtgt gccgctgctg cgcatggtga
    10261 tcagcgacat cacagagcgc aagcgagccg aagacgcgct gaaacaaagc gaagaacgct
    10321 ggcaatttgc cctggaaagt ggcggcgacg gcctgtggga ctggaatctt cagactggcg
    10381 aggcgttctt ctcaccccgg tacaagcaaa tgcttggcta tgccgagcat gaattcgaga
    10441 acaatgctga agcctggtcc aagagcattc acccggatga tgcacccggc gtgatgacga
    10501 ctttgcggcc ctatctggat ggcaaagatg gtaccgccag cgtggaattt cgcatgctgt
    10561 gcaaggacgg cagttggcta tggacgctgg ggcgcggcaa ggtgatcggc cgcgatgcca
    10621 gcggcaaagc cacgcgcatg attggcacca atgccgacat caccgagcgc aaacaagccg
    10681 aaaaagtcat tcgtgaaagc gagagccggt ttcgctcact gatggagaac attgccggtg
    10741 tggcggtgca gggctgtgcg ctggacggca cggtgctgta ctggaacccg gcctctgagc
    10801 gcctttatgg ctacagtgcc acagaggtgc tcggggccaa tctgcttgac ttgatctttc
    10861 ccccggacat gcacgaaggt ttcaagctgg cgatgcagca aatggcaaca accggcgaac
    10921 cgattccggc cgacgaattg atgctcaaga acaaggcggg cacccccacc ccggtttttt
    10981 caagccatgc gctggttcaa cccattggac gtgagcccga gctgttttgt ctgagcatag
    11041 atctgagcga aagaaacaag gcccgtgacg cactgcagca aagtgaagcc ttcaaagagg
    11101 caatccttaa ttctgtagcc gctgaaattg tggtgattga tggcaacggg gtgattctgg
    11161 cggtcaatca gcactggcgg caatacgcgc tggacaatgg attggagccg ggccaggtcg
    11221 cgcccaacac cggtgtgggc agcaattacc tgtcgatatg cgccatgggt gcccaggctg
    11281 atgacaacgc ccggcaagcc tgcgacggca tttgggccgt gctgcaaggt cgtttgccca
    11341 gattcagtct ggagtatccc tgccactcgc ccgggcggct gcgctggttc aacatggtcg
    11401 ttatgccctt gggctacaac ggccagggtg ccagcatcac ccacaccgat gtcaccgcca
    11461 tcaagcaagc cgatgagcgc gttctgtcaa gcgaacgcca tctgcgcgca atttttgaaa
    11521 cttcgctcga cgcggtgatc ggcatggatg accagggtcg catcaccgac tggaaccagc
    11581 aggcacaaac catttttggc tggcacaagg atgaagtgat gggcctgatg ctgcacgaca
    11641 ccatcgcccc ggctcactac cgagccgcgc accatcaggg tctgaccagg tttctggtga
    11701 ccgggcaaag tgacatgttg ggtcgccgcc tggagttggt cggccagcgc cgcaatggtg
    11761 aagagtttcc gatcacgctg tcagttttac cattcaagac tggcgaaggc tatcggttca
    11821 ctgcctttgt cgatgacatc tcggagcgta agcgggttac agcggccctg caggaaagcg
    11881 agcaaaaatt caggttgatt gccgaaaaca ccagcgatgg cattaccatt tttgataaaa
    11941 atggccatgt ccaatacgtc tcgccgtcag tcatgcgaca gttgggatcc tctgaacagg
    12001 aagaacttgc ccggacaaac gccgaggtgt tttcgatcat gcatcccgat gaccgcgacg
    12061 aggtgttcgc aagaatagat gaagcgataa agaacataaa ggacagcctg acctattcat
    12121 accgggtcaa acaccagtcc ggccactatt tctggcgcga agacaacgtc agtctcatct
    12181 ttgatagcaa ccaggagtat ggcggcgctt atgtgatttc gcgcgacatc accgagcgca
    12241 agcgcctgga agaacaggtg cgccaactgg cctactttga cccgctgacg aaactgccca
    12301 accggcgcat gctgggcgac cgcctgagtc agaccatggc gctgagcaaa cgcagtggac
    12361 gctatgctgc cgtgatggtg ctggacttgg acaacttcaa gccgctcaat gacaagcacg
    12421 gccacttggt tggtgacatg ctgctggttg aagtggccag tcgcttgctt cattgtgttc
    12481 gtgcggtcga taccgtggcc cgctttggcg gcgatgagtt tgttgttgtg ctgggtgaac
    12541 ttgatgtgga tagcgctgaa tcaagggcac aggcccaggt gattgcggaa aaaattctcg
    12601 cgtccctgtc tgcaccttac gtcatgaccc gcaggaaaga cgagg
//
LOCUS       DAOSLL010000050        12630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5026_length_12630_cov_5.078012, whole genome shotgun sequence.
ACCESSION   DAOSLL010000050 DAOSLL010000000
VERSION     DAOSLL010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 12630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12630
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5026_length_12630_cov_5.078012"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..521
                     /locus_tag="PLB25_03540"
     CDS             30..521
                     /locus_tag="PLB25_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HOE40694.1"
                     /translation="MLAQVLAEGVATVADEQQMPGVAAAAAPVFNFKRGIVLAILVKG
                     VKDLIDLSANGTVVTAVTRCAHNLSQRLSQHGGSWSHRQPKKTGGPMVSLYFVKKYAF
                     SPYLTCVGSNYIDSIMTLVTGPFGAGQSRQTPLDRVNSMFQNPALKQCTIPPSSKTSV
                     KTC"
     gene            571..1446
                     /locus_tag="PLB25_03545"
     CDS             571..1446
                     /locus_tag="PLB25_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOE40695.1"
                     /translation="MLAEFLQFLFSGITVGATYALAALGFTLIYNASNVINFAQGEFI
                     MLGGMLAVFFTQAGLSMPVALLLAIAVPAIVGMLVEKLAIEPVKGAESVTLIIITIGA
                     SLVIRGLIQVWLGKGTFSLPAFSGDTPLHFLGATLMPQSLWVLGVTALVVVALWYFFN
                     RTLHGKAMLATSHNRLAAELVGINTDRVMLLSFAMSAALGALGGILVTPITLTSYDVG
                     IMLGLKGFVAAVVGGLGNGLGAVLGGLLVGILEAMGAGYISSSYKDAMPFVLILLILF
                     FMPRGLFGGQSTERV"
     gene            1449..2384
                     /locus_tag="PLB25_03550"
     CDS             1449..2384
                     /locus_tag="PLB25_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOE40696.1"
                     /translation="MAKHPFAGLYLLMAVLLLLPFVLPNSFYVDLAIRMAINAMIVLG
                     LNLLIGFAGQISLGHAGFLGIGAYATAVLPTHFGWHPLLALGGGALAAGLLAALVARP
                     IFKLKGNYLAMATLGLGIIINITVRNEAALTGGPDGMPVPPLSLAGLEISGDKHWYWI
                     VALMLSVSVWASLNLIDSPFGRALRALHGSEVASKVVGVDVVRYKVAIFVLSAVFASV
                     MGSVTAHYVGFVTPNLADFFHSIELVTMVVIGGMASVYGSVVGAVLLTALPQALTSFE
                     GWETVAFGTILMLCMIFLPKGLVPTLATRFDRGAD"
     gene            2384..3145
                     /locus_tag="PLB25_03555"
     CDS             2384..3145
                     /locus_tag="PLB25_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE40697.1"
                     /translation="MALLEVKDLSIHFGGVKAVQNVSFSIDAGIVYAVIGPNGAGKTT
                     LFNLITGIYTPTSGAIVLDGQSIVGQSPDALAQRGVARTFQNLQICMNMSAIENVMVG
                     AHLRLDRNLLKACLRWPSLRQRDRDLHQEAAGLMRFVGLEAFVNARADSMPYGALKRL
                     EIARALAMKPRLIFLDEPAAGLNPRETIEVDGLVRKVADSGVTVVLVEHDMKMVMNLS
                     DRILVLDYGKKLTEGTCAEVRNNPDVIAAYLGAHA"
     gene            3159..3557
                     /locus_tag="PLB25_03560"
                     /pseudo
     CDS             3159..3557
                     /locus_tag="PLB25_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013936.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
     gene            3774..4475
                     /locus_tag="PLB25_03565"
     CDS             3774..4475
                     /locus_tag="PLB25_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003810743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE40698.1"
                     /translation="MLRVEGMQSCYGRIKALKGITLSVNRGETVALVGANGAGKTTFL
                     RTLSGVQPMSAGSIHFDGEDISQLRSDKRMRRGICQSPEGRQVFGPLSIEDNLRLGAY
                     TQPRHQVGSDIDKIFAMFPILKEKRALPAGTLSGGQQQMLAIGRALMGRPRMLLLDEP
                     SMGLGPLLVQEVFEVIKTLKSQGMTILLVEQNALAALDIADRAYVLETGSVTLTGTGQ
                     ELISNEQVRAAYLGM"
     gene            5117..5377
                     /locus_tag="PLB25_03570"
     CDS             5117..5377
                     /locus_tag="PLB25_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40699.1"
                     /translation="MGQVTIYVEDSALDAAKRAAERAKVSVSQWFGKFAIEERQRQSQ
                     SWDAFFAEIDHLRVPDEGPDGWDALLLDRNAGLGKDTPRESF"
     gene            5374..5772
                     /locus_tag="PLB25_03575"
     CDS             5374..5772
                     /locus_tag="PLB25_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOE40700.1"
                     /translation="MKYLLDTDTLIYTFKRAGNCLARLSQQPDADLAVSCINLFELEF
                     GMVKSNNHTLMERYLREICSRYTVLDFDHAAARQGGLVRAHLQSLGTPIGPYDVQVAG
                     VALSRRLTLVTRNTREFDRVPDLRVENWYD"
     gene            5855..6205
                     /locus_tag="PLB25_03580"
     CDS             5855..6205
                     /locus_tag="PLB25_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40701.1"
                     /translation="MTTSTHSSSSGAKPQQLLVRLPDDLAQRFAQVVPSRQRSRFLLD
                     LLRSELDRESLALAQAAQTLTELESGQPALKAEAAQWVAASLTTDTPDDDADFNAEAF
                     ERDFAAAQAQAQNP"
     gene            6202..6546
                     /locus_tag="PLB25_03585"
     CDS             6202..6546
                     /locus_tag="PLB25_03585"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOE40702.1"
                     /translation="MTASIERGDVYWVELDPTHGAEITKTRPCFVLSASEINQHRKTV
                     VVIPLTTTATPAAPPLLIAVPSAGASSKARIEHIRAVDKSRLQRKLGRLGAQDLHAVE
                     EALRRVLRLPKD"
     gene            6672..6977
                     /locus_tag="PLB25_03590"
     CDS             6672..6977
                     /locus_tag="PLB25_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40703.1"
                     /translation="MTTPTDLLHKLEHLSAPQLRRLLTEHLTRQKLGLYWESSAIERD
                     AALNANIVLPRLVEADSHGLNKIAGPGTPNLIIEGDNFDSLAPAQKHPRRQSARDLH"
     gene            6964..8646
                     /locus_tag="PLB25_03595"
     CDS             6964..8646
                     /locus_tag="PLB25_03595"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HOE40704.1"
                     /translation="MIYIDPPYNTGNKDWVYNDRYVGQNDRWRHSQWLEFLYQRLTLA
                     KDLLTQDGVIMVSINDENRSRLELLMDEVLPGRRLGSLVWRTKDTGNDLSQRFSHVHE
                     HVLVYANPGFKFNGRATNRRKFRNPDNDLRGDWSPQPLTANKTLIERKNTYYPIQNPE
                     TGYWYPCDPDSTWRFASEKEIRQRLKGEEAAIQAALDGLRSDTMEGLIANKLIYFPPC
                     KPEDVMQFDTRAALSAAIATGTGPMLPKKKTPLLREDLPDLDFWVGKPIATGRPSRKE
                     LWTAKPEADRLAPLSSWIAGQNEAVATLEDDNGDEPEVLRSARGGVATEEIKNLLGSK
                     AFPYPKPLSLIQGLISQASRPGDTVLDFFAGSGTTGHAVLALNALEPDQPPRKFILCS
                     STEATAKEPGKNLCRDVCAARISKVIASDAALAHNSFAYLQLDRLAPGDAPFDASCVQ
                     AFQLLTLRLGQVARPVPATDQGIKILVQNTGDGVLTVLCHKLTRAALDPMAALPCRQL
                     RVYSTRPATVREHFAALGREVESLSLQKALLHGQLARRSQRQPTTQNEPQKL"
     gene            8691..8915
                     /locus_tag="PLB25_03600"
     CDS             8691..8915
                     /locus_tag="PLB25_03600"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HOE40705.1"
                     /translation="MSQLEYVEAQVMQLTPAERNHLLERLINSLDTDPEVEAAWEQVA
                     DQREADLEAGLAQEIPFEEAMLQLRARLTA"
     gene            8912..9205
                     /locus_tag="PLB25_03605"
     CDS             8912..9205
                     /locus_tag="PLB25_03605"
                     /inference="COORDINATES: protein motif:HMM:NF016876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOE40706.1"
                     /translation="MKRSMHAGASQDVANAVDFYTQSAGVHIATRFLNEFERVARLII
                     DNPGFGTPTTLGKRAYPLHGFPYSVVYSAKGDTLRFLVVRHQRQRPGFGSGRH"
     gene            9219..11978
                     /locus_tag="PLB25_03610"
     CDS             9219..11978
                     /locus_tag="PLB25_03610"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOE40707.1"
                     /translation="MTLDTPITAPQSASFSPKQFQARIIHGVCGALAESPRPPCLLRS
                     PTGSGETFMLTKILENVSKTNEVLWLWFVPYVNLVAQTVDTLDSQSTETGLIARHLTV
                     ARNETPEAGQVLVSTVQGVASAKSHKAHYTDKSDDGERSLAAYFALARARGLAIGLIV
                     DEAHIALHLATEFGRFVKWLEADYLLMASATPKDAQLNDFIASAGNIAFKAFSVSRAD
                     VVAERLNKPWLETVVYDLRDSMQGITDLKLTVLRRAWRRNQHIGQLLKSRGLSTIPLL
                     LVQVANGDVAIREAYEFLTKELAIAPNAVAMHSAEEPDPVMMAAIAVNTAIEVLIFKQ
                     AAGTGFDAPRDFVLASTKSVTDEDFAMQFIGRVMRVPGELQRAFPDPYTAPAELDTAC
                     IFLANAQSQAGFADAAAATKAVMSQLEGQIEELQMRRTAHGGLHLSNHVTPQTPLGYD
                     IGLLPTLAADGTVRPPSYASNTALALVDTALTAPDIEVGATHELFGTLAQTDEFDALD
                     VMQAAGGNLAAPKRQSPANRTELLAALETCGLRAYPRLGEVGKRPTPQQFTTEVKPVF
                     DVLELDIREAARTLPLSDEVKSVATRAALNLLTEREIRTELFSGDSFEESVQVVTDRT
                     ALMQRTEEALASMGLEEQDLIDLVGELGRRLLPEIERAHAYREDALRPPPATLRTQAR
                     DAACWVVHKLLPELQEALQAQWANKAGEVCAQPLPDALLMPASQPLQPSRKNIYGVLC
                     PDTDAVALAANALPMTAAKVFKNETYVFAQDAQGRPTGDVFSTARLDATYEFNTQELA
                     FAKALDRADFVVWWHRNPQGKPYSIALMRSDNRNMFYPDFVVCLTHQPGDEPLTRLVE
                     TKHDTKDANRKARHTSKTYSKVLFLTKDAQRFKIVQDDGSLGEVVDFDDLARLRQWMR
                     DTAPV"
     gene            complement(12200..>12630)
                     /locus_tag="PLB25_03615"
     CDS             complement(12200..>12630)
                     /locus_tag="PLB25_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40708.1"
                     /translation="PNPPVQIGEVISDHILSHVPDTTGILQHSLKNPNVPSAMTYAWV
                     GPFVGALIRPVGSWISDKVGGSIVTQVISAIMVGASVGVGYEMMQAYGSATPAKYSST
                     FRAIGVFLPPASRAGAGLYLCGCRLRCLCRPGGDWCADQG"
BASE COUNT         2589 a         3746 c         3771 g         2524 t
ORIGIN      
        1 agttgcactc ggccggtggc gtggctgcca tgctggcgca ggtgctggcc gagggtgttg
       61 ccaccgtggc cgatgagcaa cagatgcctg gtgtggcggc ggcggccgca ccggtgttca
      121 acttcaagcg cggcattgtc ctggcgattt tggttaaggg tgtcaaggat ctgattgacc
      181 tgtcagccaa cgggactgtc gtcactgctg taacccgatg cgcccacaac ctgtcgcagc
      241 gcctgagcca gcatggcggg tcttggtctc accgccagcc aaaaaaaaca ggcggaccga
      301 tggtttccct ctattttgtg aagaaatatg ccttcagccc ttatctcacc tgcgttggca
      361 gcaactatat tgatagcatc atgacgctcg tgaccgggcc ttttggggcg ggacaatccc
      421 gacagacgcc acttgatagg gtaaattcca tgtttcagaa tccggccctt aaacaatgca
      481 ccatcccgcc aagttcaaaa acaagcgtca agacatgttg agttggtcaa cccgccgcag
      541 gtggcttgac ctgatgaccc ggagcgtttg atgctggccg agtttctgca gtttcttttt
      601 tctggcatca ccgtcggtgc cacctatgcg ctggcggcgc tgggcttcac actgatctac
      661 aacgccagca acgtcatcaa ctttgcccag ggcgaattca tcatgctcgg cggcatgctg
      721 gcggtgtttt tcacacaggc cgggttgtca atgccggtgg cgctgctgct ggcgattgcc
      781 gtccccgcca tcgtcggcat gctggtggaa aagctggcga tcgagccagt caaaggggca
      841 gagtcggtca ccttgatcat catcacgatt ggtgcctcgc tggtgattcg tggcctgatc
      901 caggtctggc tgggcaaggg caccttcagc ttgccggctt tttcgggcga tacaccgctg
      961 cattttctgg gtgccacgct gatgccgcaa agcctgtggg tgctgggcgt gacggccctg
     1021 gtggtcgtgg cactgtggta tttcttcaac cgcacattgc acggcaaggc catgctggca
     1081 acctcacaca accgtctggc agccgagctg gtcggcatca atactgacag ggtgatgctc
     1141 ttgagttttg ccatgtcggc agcattgggc gctttgggcg gcattctggt gacgccgatc
     1201 acgctgacct cgtatgacgt gggcatcatg ctgggtctga aaggttttgt ggcggcggtc
     1261 gtcggcggcc tgggcaacgg cctcggtgcg gtgctgggcg ggctgctggt gggcattctc
     1321 gaagccatgg gcgctggcta tatttcgtcg tcctacaaag atgcgatgcc gtttgtgctg
     1381 attttgctga tcttgttctt catgccacgc ggcctgtttg gcggccaatc gaccgagcgc
     1441 gtctgagcat ggcgaaacac ccgtttgctg gcctgtacct gctgatggcg gtgctgttgc
     1501 tgctgccctt tgtactgccc aacagctttt atgtcgatct ggcgattcgc atggcgatca
     1561 acgccatgat tgtgctgggg ctcaacctgc tgattggttt tgccgggcag atcagcctcg
     1621 ggcatgccgg ttttctgggc attggggcct atgccacggc ggtcttgccg acgcattttg
     1681 gctggcatcc gctgctggcg ctgggcggtg gtgcgctggc tgcggggctg ctggcggcac
     1741 tggtggcgcg accgattttc aagctcaaag gcaattacct ggcgatggcc acgctgggtc
     1801 tgggcatcat catcaacatc acggtgcgca acgaagccgc cctgaccggt ggcccggacg
     1861 gcatgccggt gccgcccctg agcctggcgg ggttggagat ttcgggtgat aaacactggt
     1921 actggatcgt ggcgctgatg ctgtcagtca gcgtctgggc atcgctgaac ctgattgact
     1981 cgccgtttgg ccgggcgctg cgcgcgctgc atggctccga ggtggcctcc aaagtggtgg
     2041 gcgttgatgt ggtgcgttac aaggtagcaa tttttgtgct gtcagcggtg tttgccagcg
     2101 tgatgggcag cgtcaccgcg cactatgtgg gttttgtcac gccgaatctg gctgattttt
     2161 tccactcgat cgagctcgtc accatggtgg tgattggcgg catggcctcg gtctatggct
     2221 cggtggtggg tgccgtgctg ctgacggcgc tgccgcaggc gctgacctcg tttgaaggct
     2281 gggaaaccgt tgcttttggc acgattttga tgctgtgcat gatctttttg cccaagggtc
     2341 tggtgcccac attggcgacc cgctttgacc ggggggctga ttgatggcac tgctggaagt
     2401 caaagacctg tcaatccatt ttggtggtgt caaggcggtg caaaacgtca gtttctcgat
     2461 agacgcaggc attgtttacg cggtgattgg ccccaacggg gctggcaaaa ccaccttgtt
     2521 caacctgatt accggcattt acacgccaac atcgggcgcc attgtgctgg acggccaatc
     2581 gattgtcggc cagtcgcccg acgcgctggc ccagcgtggc gtggcccgca ccttccagaa
     2641 cctgcaaatt tgcatgaaca tgagcgccat cgagaacgtg atggtcggtg cccacctgcg
     2701 actcgaccgc aacctgctca aggcctgttt gcgatggcca tcgctcagac aacgcgaccg
     2761 ggacctgcac caggaggcag ccgggctgat gcgttttgtt ggactggagg cgtttgtcaa
     2821 cgcccgcgcc gacagcatgc cctatggtgc cttgaagcgg ctggaaatag cccgtgcgct
     2881 ggcgatgaaa ccacggctga tctttctcga cgagcccgcc gccggcctga accccaggga
     2941 aaccatcgag gtcgatgggc tggtgcgcaa ggttgccgac tcaggggtca cggtggtgct
     3001 ggtggagcac gacatgaaga tggtgatgaa cctgtcggac cgcattctgg tgctcgatta
     3061 cggcaaaaag ctcaccgaag gcacttgtgc cgaagtgcgc aacaaccctg atgtgattgc
     3121 cgcctatctg ggcgcacacg cctgacacac atagccccat ggacgcattg cgcatcatca
     3181 gcgaagacca caacaacctg tggcgactgg ccaccacggt ggatcaactg gctggcgaac
     3241 tcgaagccgg ggccgaactc gatagcgctt tttttggctc ggtgtttgac tatttcgagc
     3301 agtttgttga acgctcacac caccccaagg aggatgagca cctgtttcga ctgctgcggc
     3361 tgcgcagccc gcaagccgcc gcgacgctgg acgacctgca ggccgatcac cgtgccgggc
     3421 tggccaaatt gacggcgctg cgggccagca tgaaggccac cgccagcggt actttgccag
     3481 tgcccaatct ggcccagcaa ctgcgcgcct acacggcggg catgaaaacc cacatcctga
     3541 ccgaagaaaa acaggttttg ccgctggccc gtcaggcgct gctggcacag gactggcttg
     3601 aaatcaaccg cgcctttcac gacaacagcg acccgctgtt tggcgaggca gcgcaggccg
     3661 agttccgcga gctgtaccac cgcatcgtca gcctggcgcc cgagtcggtt ggcctgggtg
     3721 gccacagcgc cggcaatctg ccagccaaag cgcttgcggc gcaggccgat gtgttgttgc
     3781 gggtcgaggg catgcagagc tgttatggcc gcatcaaggc gctcaagggc atcacgctga
     3841 gcgtcaaccg gggcgaaacc gtggccctgg tgggtgccaa tggggccggc aagaccacct
     3901 ttttgcgcac cctgtcgggc gtgcagccga tgagcgccgg cagcattcat tttgacggcg
     3961 aggacatcag ccaactgcgt tcggacaagc gcatgcgccg tggcatctgc caaagccccg
     4021 agggtcggca ggtctttggc ccgctgtcga tcgaagacaa cctgcgcctg ggggcctaca
     4081 cccagccgcg ccatcaggtg ggcagcgaca tcgacaagat ttttgccatg tttccgatcc
     4141 tgaaagaaaa gcgtgccttg cctgctggca cgctgtcggg cgggcagcag cagatgctgg
     4201 cgattggccg ggcgttgatg gggcgcccgc gcatgctgct gctcgatgaa ccgtcgatgg
     4261 gcctggggcc gctgctggtg caagaggtgt ttgaggtcat caagacattg aagagccagg
     4321 gcatgaccat tttgctggtc gaacaaaacg ccctggcagc actcgacatt gccgaccggg
     4381 cctatgtgct ggaaaccggc agcgtgacct tgacgggcac cgggcaagaa ctgatcagca
     4441 acgaacaggt gcgcgccgca tacctgggga tgtgagctgg gcgccgcatg ctgcctgatt
     4501 ttttcgggaa ctggaacacc ttactgacca attgctcgac gcatgatcag gaaatgtgtt
     4561 cgcatcaaca gtcatcaaca gagggctcca tctcctgggc gacttgatcg ttgtcatgaa
     4621 gtgcagagtt aaccattgcc gcctgaacat tttgcggcgt ttgtatgact gttgttgtca
     4681 gttcattggg tgcgttgcgc gcagtagcat tcagttgacc aggtgagtga cttggtcaat
     4741 cggctgcggt aacccataca gataatggcc cgcatttcat cgctgcccgc caggataacc
     4801 gtctgatggc catgagcgaa gtgcatcgga aaaggacgcg ctttgtctgc gtagatgagc
     4861 tggacctctc aaagtagacc gctgtgcatg gctggctgaa gggataggtg aaagcagtac
     4921 tggtggtcag ccaagtattt tgaagcaaag acgacagcac tgggatagtg catctggtgg
     4981 gttggggtag ttcataagaa acggggatgt ggtgagaaat aacttcccga tgtcattgcc
     5041 gcgaaggcgc gcattcaggc tggtataagt gctgaacact tatacttgca tttctcttca
     5101 ctgccggagc cactccatgg gccaagtcac catttacgtc gaagacagcg cgctcgatgc
     5161 cgccaaacgc gccgccgagc gcgcaaaagt ttcggtcagc caatggtttg gcaagttcgc
     5221 cattgaagag cgccagcggc aaagccaaag ctgggacgcg ttttttgccg agattgacca
     5281 tttgcgtgtc cctgatgagg gaccggacgg ctgggacgcg ctgctgcttg acagaaatgc
     5341 gggtttgggt aaagacacgc caagggagtc attttgaaat acctgcttga caccgacacg
     5401 ctgatctaca ccttcaagcg cgctggtaac tgcctggcgc gcctgtcgca acaacccgat
     5461 gccgacctcg ctgtaagttg catcaacctg tttgaacttg aatttggcat ggtcaaatca
     5521 aacaaccaca ccctgatgga acgctacctg cgcgaaattt gcagccgcta caccgtgctt
     5581 gactttgacc atgctgcagc acgacaaggc ggcctggtgc gcgcccattt gcagtcactt
     5641 ggcacaccga ttggccccta cgacgtgcag gtggccggtg tggccctgtc gcgccgtttg
     5701 accctcgtca cccgcaacac ccgcgaattt gaccgcgtgc ccgacctgcg cgttgaaaac
     5761 tggtacgact gacaacaaaa ctggccgcca gcgctggttt caaaatttcg tcgatcatgt
     5821 atattgctat cttgataaca gataccgctt gatcatgaca acttcaaccc actcttcaag
     5881 ttctggtgca aaaccgcagc aactgcttgt gcgcttgcct gacgacctgg cccagcgctt
     5941 tgcgcaagtg gtgccctcgc gccagcgcag ccgttttttg ctggacttgc tgcgctcgga
     6001 gctggatcgg gaaagtctgg cactggcgca ggcagcccaa accctgactg aactggagtc
     6061 cggtcaaccc gccctgaaag cagaggcggc gcaatgggta gcggcctcac tcaccacaga
     6121 tacgcccgat gacgatgcgg atttcaacgc cgaagcattt gagcgcgact tcgcagcagc
     6181 ccaggcgcag gcgcaaaacc catgaccgcc agtatcgaac gaggcgatgt ttattgggta
     6241 gagcttgacc ccacgcacgg cgctgaaatc accaaaaccc gcccttgctt tgtcttgtcc
     6301 gccagcgaaa tcaaccagca ccgcaaaacg gtcgtggtca tcccgctgac caccacggcc
     6361 acaccggcag ccccgccgct gctgatcgcc gtgccatcgg ctggggccag cagcaaggca
     6421 cgcattgaac acatccgcgc tgtcgataaa agccgtcttc aacgcaagct cggccgactt
     6481 ggcgcccagg acttacacgc ggttgaggag gccttgcggc gtgtcttgcg cttgccaaag
     6541 gactgaattc aaggctttgc atcgcttgcg ttgaaccgct caagttgccg gtcagagcca
     6601 gcgaatttat aacaaatcag cctctagacc ccgtcctaat tgcgctggca gctatcaaaa
     6661 atgtatcatc catgaccacc ccgaccgacc tgctgcacaa gctggaacat ttgagcgcgc
     6721 cgcaactgcg gcgcttgctg accgaacatt taacccggca aaaactcggg ctgtactggg
     6781 aaagtagcgc catcgagcgc gacgcggcct tgaacgccaa catcgtgctg ccgcgtctgg
     6841 tggaggccga ctcgcatggc ctgaacaaaa tcgccgggcc gggcacgccc aatctgatca
     6901 tcgaaggcga caacttcgac agccttgcgc ctgctcaaaa gcacccacgc cggcaaagtg
     6961 cgcgtgatct acattgaccc gccttacaac accggcaaca aggactgggt ttacaacgac
     7021 cgttacgtgg gccagaacga ccgctggcgg cacagccagt ggcttgaatt tttgtaccag
     7081 cggctcacgc tggccaagga tttgctgacg caggacggtg tcatcatggt cagcatcaat
     7141 gatgaaaacc gttcaaggct tgagttgctg atggacgagg tgctgccggg gcgacgccta
     7201 ggtagcctgg tgtggcgcac aaaagacacc ggcaacgacc tgagccagcg ctttagccat
     7261 gtacatgagc atgtgctggt gtatgccaac cccggcttca agttcaatgg ccgcgccacc
     7321 aaccgccgca aattccgtaa cccggacaac gacttgcgcg gggactggtc accacaaccg
     7381 ttgacagcaa acaaaactct cattgaacgc aaaaatacct actacccgat tcagaacccg
     7441 gaaaccggct attggtatcc ctgcgacccc gacagtacct ggcgatttgc ctctgaaaaa
     7501 gaaatccgtc agcgcttgaa gggtgaggaa gccgccattc aagccgcact cgacggcctg
     7561 cgcagcgaca cgatggaagg cttgattgcc aacaagctga tttacttccc gccgtgcaag
     7621 cccgaggacg tgatgcagtt tgacacccgc gctgctttga gcgccgccat tgcaacgggc
     7681 accgggccga tgctgcccaa aaagaaaaca ccgctgttgc gcgaagactt gcccgacctt
     7741 gatttttggg tcggcaaacc gattgccacc ggtcgcccct cgcgcaaaga gctgtggacg
     7801 gccaagcctg aagcagaccg gctggccccc ttgagcagtt ggattgccgg acaaaacgaa
     7861 gcggtagcca cactggaaga tgacaacggc gacgagcctg aagtgttgcg cagtgcgcgt
     7921 ggcggcgtgg cgacggaaga aatcaagaat ctgctgggtt ccaaagcctt tccgtacccc
     7981 aaaccgctgt ccctcatcca gggcttgatc agccaggcca gccgtcccgg cgacaccgtg
     8041 ctcgatttct ttgccggcag tggcaccacc ggccacgcgg tgctggcgct caatgcgctg
     8101 gaacccgatc agccaccgcg caaattcatt ttgtgcagca gcacagaggc caccgccaaa
     8161 gaacccggca aaaacctgtg ccgcgatgtc tgcgccgccc gcatcagcaa ggtgattgcg
     8221 tctgatgccg cgctggcgca caacagtttt gcctatctgc agcttgaccg gctcgccccc
     8281 ggtgatgcgc cgtttgatgc cagttgcgtg caagcctttc agttgctcac gctgcgcctg
     8341 ggccaggtgg cccgaccagt gcccgccaca gaccaaggca tcaaaattct ggtgcaaaac
     8401 acaggcgacg gtgtgctcac ggtgctgtgc cacaagctga cccgcgccgc gctggatccc
     8461 atggcggcgc tgccatgccg ccaactgcgc gtgtactcga cccgccccgc caccgtgcgc
     8521 gagcattttg ctgcgctggg gcgcgaggtg gaaagcctct ccttgcaaaa agcactgctg
     8581 catggacaac ttgcgcggcg gagccagagg cagccaacga cgcaaaacga gccgcagaag
     8641 ttatgatttt cccccgccaa acccttttaa ccacaccgga ggttcccatg atgagccagc
     8701 ttgaatatgt agaagcccaa gtgatgcaac tcacccctgc tgagcgcaac cacttgctgg
     8761 agcggctgat caatagcctt gacaccgacc cggaggtcga agccgcctgg gaacaagtgg
     8821 cggatcagcg cgaggcggac ctggaagccg gtttggcgca ggaaatcccc tttgaagagg
     8881 ccatgctcca actgcgtgcc cggttgaccg catgaaacga tccatgcacg ctggcgccag
     8941 ccaggatgtc gccaatgcag ttgactttta cacacaaagt gccggtgtac acattgccac
     9001 ccgttttctg aatgagtttg agcgggtggc acgtctcatc atcgacaacc cgggttttgg
     9061 cacacccaca acacttggca aacgcgccta ccctctgcat gggtttccct attctgtggt
     9121 ttacagcgct aaaggcgaca cactgcgttt tttggtcgtt cgtcaccaac gccaaagacc
     9181 aggtttcggc agtggcaggc attgaccggc cacccgacat gacgcttgac acccccatca
     9241 cggcgccaca gtcggccagt ttttcaccca agcagttcca ggcgcgcatc attcacggcg
     9301 tgtgtggggc attggcagaa agtccgcgcc cgccttgcct gctgcgcagt cccacggggt
     9361 cgggcgaaac cttcatgctg acaaaaatcc tcgaaaacgt cagcaaaacc aacgaggtgc
     9421 tgtggctgtg gtttgtgccg tatgtgaacc tggtggcgca aactgtggac acgcttgaca
     9481 gccaatcgac cgagaccggg ctgattgcca gacatctgac agtagcccgc aacgaaaccc
     9541 ccgaggccgg gcaggtgctg gtatcaaccg tgcaaggcgt ggccagcgcc aaatcgcaca
     9601 aggcgcacta caccgacaaa tctgacgacg gcgagcgcag cctggcggcc tactttgcgc
     9661 tggcgcgcgc acgcggtctg gccattgggc tgatcgtcga tgaggcacac attgcgctgc
     9721 acctggccac cgaatttggc cgctttgtaa aatggctgga ggccgactat ttgctgatgg
     9781 ccagcgctac gcccaaagac gcgcagctca acgactttat tgccagcgcc ggcaacatcg
     9841 cgttcaaggc cttcagcgtg agccgcgccg atgtggtggc cgagcggctc aacaagccct
     9901 ggctggaaac cgtggtgtat gacctgcgcg actcgatgca gggcattacc gacctgaagc
     9961 tgacggtgct gcgccgggcc tggcggcgca accagcacat tggccagttg ctcaaaagcc
    10021 gtggcttgtc caccatcccg ctgctgctgg tgcaggtggc caatggcgat gtggccattc
    10081 gtgaagccta tgagttttta accaaagagc tggcgattgc gcccaatgcg gtggccatgc
    10141 attcggctga agagcccgac ccggtgatga tggcagccat tgcggtcaac accgccatcg
    10201 aggtgctgat cttcaagcag gccgcaggca ctggctttga tgcgccgcgc gactttgtgc
    10261 tggcatcgac caaatcagtc accgatgaag actttgccat gcagttcatt ggccgggtga
    10321 tgcgcgtgcc gggtgaattg cagcgggctt ttcctgaccc ctacaccgca ccggcggagc
    10381 tggacacggc ctgtattttt ctggccaatg cgcagtcgca ggccggtttt gccgacgcgg
    10441 cggccgccac caaagcggtg atgtcgcaac tggaagggca aattgaagag ttgcagatgc
    10501 gccgcaccgc gcacggcggc ctgcacctga gcaaccacgt caccccgcaa acgccgctgg
    10561 gttatgacat tggcttgctg ccaacgctgg ccgctgatgg cacggtgcgc cccccttctt
    10621 atgcatcaaa tacggctcta gccctcgtgg atactgcgct gacagctcct gatattgaag
    10681 taggtgcgac gcatgagctg tttggcaccc tggcgcaaac agacgagttt gacgcgctgg
    10741 acgtgatgca agcagccggc ggcaacctgg cagcccccaa acgccagtca cccgccaacc
    10801 gcactgagct gctggccgcg ctagagacct gtggcttgcg tgcataccca cgattgggcg
    10861 aagtgggtaa gcggcccact ccgcagcagt tcaccaccga ggtcaagccg gtgtttgacg
    10921 tgctggagct ggacattcgt gaggctgcac gcacgctgcc cctgtctgac gaagtcaaaa
    10981 gtgtcgccac ccgcgccgcg ctaaacctgt tgaccgagcg cgaaattcgc accgaactgt
    11041 tcagcggtga tagctttgag gaaagcgtgc aggtggtgac cgaccgcacc gccttgatgc
    11101 agcgcaccga agaggcgctg gccagcatgg gcctggaaga gcaagacctg atcgatctgg
    11161 tgggcgaact aggccgtcgc ctgttgcccg agattgaacg cgcccacgcc taccgtgaag
    11221 acgcgctgcg cccgccacct gccaccctgc gcacccaggc gcgtgatgcg gcctgttggg
    11281 tggtgcacaa actgttgcca gaattgcaag aggcgttgca ggcgcagtgg gccaacaaag
    11341 cgggtgaagt gtgcgcccag cccttgcccg atgcgctgct aatgcccgcc agccagccgc
    11401 tgcaaccctc ccggaaaaac atctatggcg tgctgtgccc ggacaccgac gcggtggcgc
    11461 tggctgcgaa cgctctgccc atgacggcgg cgaaggtgtt caaaaacgaa acctatgtgt
    11521 ttgcgcaaga tgctcaaggg cggcccacgg gggatgtttt cagcacggcg cgccttgatg
    11581 ccacttatga gttcaacacc caggaactgg cgtttgccaa agcgctggac cgcgccgact
    11641 ttgttgtctg gtggcaccgc aatccgcaag gcaagcccta cagcattgcc ctgatgcgca
    11701 gtgacaaccg caacatgttt tatcccgact ttgtggtttg cctgacgcac cagcccggcg
    11761 acgagccgct gacccggctg gtagaaacca agcacgacac gaaggatgcc aaccgcaagg
    11821 cccggcacac ctccaaaacc tacagcaaag tactgttttt gaccaaagat gcccagcgtt
    11881 tcaaaattgt gcaagatgat ggcagcctgg gcgaggtggt tgactttgac gacctagccc
    11941 ggttgcgcca gtggatgcgc gacaccgctc cagtttaagt tttataggcc atattggcct
    12001 ctagcgctta tggataatgc gctggcagct ataaatacaa gagtagttta tcaatcaggt
    12061 cgggttgccg tagcggcacc caacgcgggc tgttcaaggg attttgatct ctgagccgct
    12121 gcgcgggtaa aaccaccagt tcagcacgag gcacagcagg taaaagatgg caaacccgta
    12181 cattgcggcc tgcggtgtgt cagccttgat ctgcgcacca atcaccaccg gggcgacaaa
    12241 ggcaccggag gcggcaaccg cagaggtaca gcccagcacc ggcccggctt gctggcggta
    12301 aaaaaacgcc aatggcgcgg aaggtcgaag agtattttgc tggcgtggcc gagccatagg
    12361 cttgcatcat ttcgtagccg acacccactg aggctcccac catgatggcc gagatcacct
    12421 gggtcacgat ggagccaccg accttgtcgg aaatccagct acccaccggg cggatcagcg
    12481 cgcccacaaa gggaccaacc caggcatagg tcattgcaga aggtacattc ggatttttca
    12541 ggctgtgctg caggatgcca gttgtgtcgg gcacatggct taggatgtgg tcagaaataa
    12601 cttcccctat ttgcaccggc ggatttgggc
//
LOCUS       DAOSLL010000051        12622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5028_length_12622_cov_4.520411, whole genome shotgun sequence.
ACCESSION   DAOSLL010000051 DAOSLL010000000
VERSION     DAOSLL010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 12622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12622
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5028_length_12622_cov_4.520411"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(306..686)
                     /locus_tag="PLB25_03620"
     CDS             complement(306..686)
                     /locus_tag="PLB25_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40709.1"
                     /translation="MQIKVLRRFVWYLTQAGERGRCLQSAVQPDQGRTNPPEGLGLDA
                     VAWLSQLKNACRIRALRRQALVGKGVTFHELTVNVANLSWSFSGVSSSKRGSISVGYN
                     KQSSGATATQNGMDRSVAGLKRRH"
     gene            complement(696..1772)
                     /locus_tag="PLB25_03625"
     CDS             complement(696..1772)
                     /locus_tag="PLB25_03625"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOE40710.1"
                     /translation="MQGFRNEKVTFTRFIAAVAIVIFHFCSDVFPFNAEPISFIFKSA
                     YVGVSYFFTLSGFIMILAYSNCKRIDALGYYKSRFARIAPAYYLALVLCVPLIIEKWA
                     EYSYSGIALNITMLQAWNPSKALSGNSPSWSLSVELLFYFLFPFLFNFVYQSRKFWII
                     VIVGGLFFLISQVIHNSLINSGVYQNFPLFRHNLIFYFPPMHLNEFLSGNIAGLFYMK
                     YIANKNSANGFWIILSIIAFCIVLKYPTGLSIHNGLLVLVFIPIIILISSDNGLIARI
                     MSLKPLIFLGEISFGIYIFQMPIHEISYAIFKIFDISNYSVLFFSYLAILVLVSGLSF
                     KFIETPLRRRINEISMRPGRLSDF"
     gene            complement(1888..2097)
                     /locus_tag="PLB25_03630"
     CDS             complement(1888..2097)
                     /locus_tag="PLB25_03630"
                     /inference="COORDINATES: protein motif:HMM:NF019228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40711.1"
                     /translation="MVSVEMIVNLIGPTKTKTGLNSQTTVDANAHTKGIKTTGKKMGV
                     LNIHKNGFRGESNCTRNPMIIDEVL"
     gene            complement(2213..3034)
                     /locus_tag="PLB25_03635"
     CDS             complement(2213..3034)
                     /locus_tag="PLB25_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40712.1"
                     /translation="MRICDGGQNIADEHQSAGQHAQHGDSGISTIALTFLGLLGVMRG
                     IVAQCKPAKQGWQVICQHLGGDIDNQSLLTQTKTPAKQLGSLGQATTRHPAPNTPTHA
                     STSPARRYRHGEHGHAKSTRSQVSKAQTTADRYWRRRACGDTREWQVSAPASAATNRA
                     PRQCLTMQSAYVRPITESLSEFSAGVCMCEQLGKGSVRYATNHFGAAAAYAGVTTGEE
                     GNKKGLLGACGHRQQCCNERWQWQLAVSGKGIGKTGMLLPVGEMHPSQSDQTNPA"
     gene            complement(3332..3667)
                     /locus_tag="PLB25_03640"
     CDS             complement(3332..3667)
                     /locus_tag="PLB25_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40713.1"
                     /translation="MGALASVGDTAYLWSPTGWVYAIDVISGAVRWNHQSTRYGHQVA
                     IWGPMMADLLTFENKLHCLDMNQELHVHEVPTGPDVQRAKFPLDQPPRCWCGQNSKRL
                     RPTRQLIST"
     gene            3639..3977
                     /locus_tag="PLB25_03645"
                     /pseudo
     CDS             3639..3977
                     /locus_tag="PLB25_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006579633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(4018..5139)
                     /locus_tag="PLB25_03650"
     CDS             complement(4018..5139)
                     /locus_tag="PLB25_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE40714.1"
                     /translation="MKAPPVVLVVDDQPENLAILGELLEPFYRVKVANSGDRALRAAV
                     SLPPPDLILLDVMMPDLDGYDVLARLRDNPLTLHIPVIFVTARDAASEEEKGLELCAA
                     DYIAKPIRPRLALARVKAQLENKLARDWLADQNAFLEKEVLRRMHDNELVQDASLHAL
                     AILAETRDVETGHHIHRTQAYVEVLARSICERPAYQRLLSDEQLKLIVRAAPLHDIGK
                     IGIPDHILRKPGRLTATEFEVIKTHSQIGGDAFALAIQRVRDADRSIYPVDAYPLAFL
                     DVARQIAMWHHERWDGNGYPDQLKGQAIPISARIMALADVFDALTSRRIYKESMPVSN
                     AIDVILSEKGGHFDPALVDVFSERRMEFAAIARRLADAT"
     gene            complement(5136..8537)
                     /locus_tag="PLB25_03655"
     CDS             complement(5136..8537)
                     /locus_tag="PLB25_03655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE40715.1"
                     /translation="MRLAQLSIRTLSLYGFGLLIVGALISGITVAYLIFDYSAIVARQ
                     RAVDDSYKSVLALKYHTERLLSTPELAKQRHQWVASVDEFEGKLPELRQAFAVEMLAI
                     DKAWKSIRIEIDEMSRQFMSPVFSEGSLMEKSLLRRLGEGLNANETGEYYVAVRTLVN
                     SIDFLQQRQNFLLDDLKQLNDRVRRQSDQQLRQTSRLLVLVSLASFFALALFAAVMFY
                     LIGRIERQLLKSQNNLRHTLDTLEFEQAQMRTLVATIPALIWLKDAQGVYLACNPPFE
                     RLYGAKESEILGKTDYDFVDRQTAEFFRLNDRLAAQAGSPSINEEWLTFKADGYRGLF
                     ETTKTPMLATDGSLIGVLGMAHDITERRSTQDELMRHRDHLEELVQARTAELAEAKVV
                     AEEANRAKSAFLANMSHEIRTPLNAIIGLTYLLRRDAVVQRQIGQLDKVTESARHLLG
                     VINDILDFSKIEAGKLTLESADFELEALFRTVYDMIGDQAASKGLEVVLDIDPDLPRH
                     LIGDRMRLGQILINFATNAVKFTAHGHVILSAVCLSADLTQCAIRFEVTDSGIGLSTE
                     QQARLFQAFEQGDASTTRKFGGTGLGLAICRRLAEMMGGSVQARSEFGRGSTFSLEAL
                     FALQPVESEVELVPVPSIVGDHTRRILVVDDHPESTDVLMRLLQRFGMEPTVVSSGAA
                     AIAGVAQAVAVGMPFDLALVAAQLPDMSSNQAVEGIRESADQVAPKVILMLGHGAEQP
                     TELLRLGVVVCMMHKPVTASSLYDALTEVLTGKCAHRHLPSAGIRLDQAQFKGRRVLL
                     AEDNPINQAVALDLLAEMGLAVDLAQDGEIAVNKAKDISYDLILMDIQMPRKDGLDAT
                     RDIRRLPERQQVPILAMTANVFDDDRQACLSVGMNDHVAKPIDPDALVKALLRWLPST
                     SGADQVSVPPVASLQETRDAPALQALRAIPGLDVDAALHLVRGKLPSYWRVLGLFVEG
                     HADAATTLQSLIDRQDFSAAERAAHTLKGSAGNVGAQAIGQLAAALERAIKQQSRNDL
                     TGLLDLLAIELPVFIAAVQAAMPTTALIPMPNQPTAKSDADLLRVVASLRALLEADDI
                     GARRFFEQHRQELTDILGLVAAANLGKMLEQFLFEEALALLNDQK"
     gene            complement(8551..10032)
                     /locus_tag="PLB25_03660"
     CDS             complement(8551..10032)
                     /locus_tag="PLB25_03660"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE40716.1"
                     /translation="MKLYVLAALSLLLVSGCGDKAAEPPAKPAIRPFEGQSITVIVPT
                     LDARLIRGPILDEVEGFEARTGAKVRVLTPGWNETIEKIDQSIKSPDASFDIYVVISM
                     WSGTLLGGGHIEPVPDAIKKKIDWDDVLPIYRNTVLSWNNVAYGLPYDGDCINLYYRK
                     DLFARPDYQARFKKKYKYDLAPPANWKAYRDVAEFFTGWDWSGDGKPDYGIAGNRVKG
                     DISMLQFFAQAAAHAKHPDDPAYYFDQNTLKPRVNNPGFARALGIWIDTLKFGPPGMA
                     NFAGHDVRNSFVNGQTAMAIDWADMGVHAVNAPSSVIKDKIGYAQIPGSAEVFNSLTG
                     KWDQRPNQVSSISGNWTFFVSKASKNKALAFEFASHMTSPELTKKMTAMSGTAVNPSR
                     KSHFDRPEAWNQSGFSTESAKAYLEEITKSLSNPNVVYDITIPGASEYYQVFDEYVYK
                     AVSGELSPAKALDQAAAQWEKITDRLGRDKQIAFYQASLNVGK"
     gene            10587..11582
                     /locus_tag="PLB25_03665"
     CDS             10587..11582
                     /locus_tag="PLB25_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOE40717.1"
                     /translation="MTLKSILAAALLAVSLGTSAQTIIKFSHVVAADTPKGKASVFFA
                     QKAAELTKGKVKVEVYANSALYKDKEEMEALQIGSVQMLAPSLAKFGPLGVKEFEAFD
                     LPFIFDDTADLHKVTQGPVGAALMAKLEPRGIKGLAYWDNGFKSFSANAPLKAVADYK
                     GKKFRIQSSKVLEEQIRAIGGIAQVMAFSEVYQALQTGVVDGTENPISNFYTQKMHEV
                     QKHLTLTNHGYLGYAVIVNKKFWDALPADVRGQLEQAMKESTVYANKIAQEENDLSLD
                     GVKKSGKTAVYVPTKEERLALKKIMAPVHAKMADRVGKETLQEIYKATGFDPNTL"
     gene            11636..12346
                     /locus_tag="PLB25_03670"
     CDS             11636..12346
                     /locus_tag="PLB25_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOE40718.1"
                     /translation="MKFLDHLEEWLVTFLMGGATLIIFVAVLHRYAAGMAIPGVQDWL
                     LSISMSWAQELTIIMFVWMCKFGAAYGVRTGIHVGVDVLINRLNKQNRGKFVIFGLCA
                     GVLFTGIVAVIASGFVWENGAHHFWLNLLGRSVEGVPEGPTTPDLEWPTWMVYSAIPL
                     GTSLMCFRFIQVLVMFVKTGDLPHHDHGHVDGLEVDPDDPLDNPYAMADNLHPRDLHH
                     SLEGKTRHHDATDGSQSK"
     gene            12343..>12622
                     /locus_tag="PLB25_03675"
     CDS             12343..>12622
                     /locus_tag="PLB25_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOE40719.1"
                     /translation="MNAAIIFFLLMVLMLTGMPISISLGLTVLTFLFGFTNVPLESVA
                     LKLFTGIEKFEIMAIPFFILAGNFLTHGGVARRMINFASSMVGHWYGGL"
BASE COUNT         2903 a         3559 c         3287 g         2873 t
ORIGIN      
        1 gcggtgccag cgcttttgac cgtaccccag gcgcgctaca ccacggcact gatcgtgagg
       61 ctgccagcct gcccttggca acgatcaaga aataccatta aaaatcaaca acttcagaca
      121 tactgatccg gtcggaatcg aagttgcaat ttcataccag tgtgtagatc atcttggtac
      181 cggtttcggg gtagcgcaat tcctcagagg acgggtatgc caatggtgcg acgatggctt
      241 tgtcatgtgc aggcgttgcc attctgtgga ttgtgatgct ttgggtcgta gtcgcatcat
      301 cgggttcaat gtcgccgctt gagaccagca actgaccggt ccatgccgtt ttgtgtggcg
      361 gttgctccgg aggactgttt gttataaccc actgatatag aacctcgctt agaactacta
      421 acaccagaaa aggaccaact gaggttggca acgttcacgg tcagttcatg gaaggtgaca
      481 ccttttccaa ccaaggcttg tcgcctcaaa gccctgattc gacaagcatt tttcaattgt
      541 gagagccatg caaccgcatc aaggccaagc ccttcgggtg gattggtccg gccttgatcc
      601 ggctgcacgg cagactgcaa acatcgacca cgctcgccag cctgagtcaa gtaccacacg
      661 aaacgacgaa gcaccttaat ttgcaagcaa attacctaaa aatccgacaa cctgccaggc
      721 ctcatagaaa tttcattaat tcttcttcgc aatggtgtct caatgaattt gaaacttaaa
      781 ccagaaacca gcaccaagat cgccaaatat gagaaaaaaa gtacactgta attggatata
      841 tcaaatattt taaatatcgc gtatgatatt tcgtggattg gcatttgaaa aatataaatt
      901 ccaaagctaa tttcacccag aaaaattaaa ggctttaatg acattattct tgcaataagt
      961 ccattatccg aagagatgag tattattatt ggaataaata caagtaccaa caaaccattg
     1021 tgaattgata atcctgttgg gtattttagc actatacaaa aggcgatgat cgacagaatg
     1081 atccagaacc cgttagcact gtttttattc gcaatatatt tcatgtaaaa gagaccagca
     1141 atatttcctg ataaaaactc atttaaatgc attggtggaa agtagaaaat tagattatgc
     1201 cgaaacagtg gaaagttttg ataaaccccc gaattgatca gactattatg tatcacctgg
     1261 cttatcaaaa aaaataaacc tccaacaatt acaataatcc aaaatttcct cgactgataa
     1321 acaaaattga acaagaatgg aaaaagaaaa taaaacaata attcaacaga caatgaccaa
     1381 cttggtgaat ttcctgacag agcctttgat ggattccaag cctgcaacat tgtaatattc
     1441 agagctattc cactgtacga atattctgcc catttttcaa ttattaatgg cacacataaa
     1501 actagcgcta aataataggc tggcgcaatc ctggcgaatc gactcttata atatccaagt
     1561 gcatctatcc ttttacaatt tgaatacgcc aagatcatga taaatccaga aagtgtgaag
     1621 aagtagctaa ctccaacata cgcactcttg aaaataaacg agatcggttc tgcattaaat
     1681 gggaaaacgt cactgcaaaa atgaaatatg acgatggcta cggccgcaat aaatcgcgtg
     1741 aaagttacct tttcatttct aaacccctgc attgatagcc ttttctggtt ggtagctctt
     1801 gtaaccgttt ggttactatc ccgtcaccct agaatttgta acacgaatgc gccactgtaa
     1861 atatattcat acgcagctta ggatgtgtta caaaacttca tcaattatca tagggttgcg
     1921 tgtgcagttc gactccccac ggaagccgtt tttgtggatg ttcagtaccc ccattttttt
     1981 acctgtggtt ttgatgcctt tggtgtgcgc attggcatcg acggtagttt ggctgtttag
     2041 accggttttc gttttcgtcg gtccgatcaa attgacgatc atctcgacgc ttaccagcgg
     2101 tttgcccgat gcaccaaact taagcgattc attgtaaaaa gcagcttgta acttgttgat
     2161 ttatatgatg ttttatcatg tctgactcag taccgtgaat ttttacagca atttatgctg
     2221 gatttgtctg atctgattga ctcggatgca tttccccaac aggcaaaagc atcccagttt
     2281 tcccaatgcc tttacccgag accgcaagct gccactgcca gcgctcattg caacactgct
     2341 gtcgatgccc gcaagctccc agcaagccct tcttgttacc ttcttcacca gtcgtcacgc
     2401 cagcataggc agcggcggca ccaaaatggt tcgtggcgta tctgacagag ccttttccaa
     2461 gttgctcgca catgcatacg ccagcactga attcgcttag cgactcggta attggtcgca
     2521 cataagctga ttgcatcgtc aagcactggc gcggcgcgcg gttggtggca gccgatgccg
     2581 gtgctgatac ctgccattcg cgagtgtcgc cgcaggcgcg tcgccgccag tagcgatcag
     2641 ccgttgtttg cgctttactc acctgactcc gagttgactt tgcatgcccg tgttcaccgt
     2701 gtcgatacct ccgagcgggc gatgttgatg catgcgttgg tgtgtttggg gccggatgtc
     2761 gtgttgttgc ttgaccgagg ctacccagtt gcttggctgg tgtcttggtc tgagtcagca
     2821 ggctttgatt gtcgatgtcg ccgccaaggt gctggcagat aacctgccat ccttgttttg
     2881 caggtttgca ctgggcgacg atccccctca tcacaccgag cagccccaaa aacgtcaacg
     2941 caatcgtgga tatgccgctg tcgccatgct gagcatgttg cccagcgctt tgatgttcgt
     3001 cggcaatgtt ctggccgcca tcgcagatac gcatggtctc ttgcacatac caacctgccg
     3061 cagtattgga ggacgctcca gccctcgacc caagcgtcat atcaaacctc acgcgcagta
     3121 tgcctacaag tgatcgcatg acttaagttc ggtgcattgg ctgtttggtg cgacaaaaac
     3181 caggaaccgc atcctatgtc aaaacatggt ccgagggcac cgtgaactga catgagcata
     3241 tttggacact tgttaacttt ccaatccttc gttcaccgca taccatgcgg gttagcccgt
     3301 acaaactcac cgcgatcttc ataccagcga atcaggtgga aatcaactga cgcgtcggtc
     3361 gcaatcgctt gctgttttgg ccgcaccagc agcgtggggg ctggtcaagc ggaaacttag
     3421 cacgctgcac atcgggcccg gtgggcacct cgtgcacatg caactcttgg ttcatgtcca
     3481 ggcaatgcag tttgttctca aatgtcagca ggtctgccat catcggtccc caaatggcta
     3541 cctgatggcc gtagcgtgtg gattgatggt tccagcgcac agcgccgctg atcacatcaa
     3601 tcgcatacac ccatccggtg ggtgaccaaa ggtaggcagt gtcgcccacg ctggccaacg
     3661 cacccatgct ggatgccgac cagcaccact tgctgcacca tgtccatctg cccagtggta
     3721 ccagttgggt gttcagttcg ctccacgcta acgtcggcct gtacctggtc ggtgcagggg
     3781 tcggcgatta tctggactcc agtcacggcg tgggctattt gatccggcag gctgaagcct
     3841 accttgacat caacaccgtg atgcctggcc tcgtggtgtt cccggcatct gcgctggcgc
     3901 tcgacagtct ggtcggacgc attgaggcac gcctgatgaa gtggcaacca cacacaactg
     3961 agaccgggat gctttgatca ttggtcagag ccgtcggtca acactacccg gcccgactca
     4021 ggttgcgtca gccagacgcc gtgcaatggc agcaaattcc atgcgccgtt cagagaagac
     4081 atcaaccaaa gcggggtcaa aatgcccccc tttctcgctc agaatgacat cgatggcgtt
     4141 gctgaccggc attgattcct tgtaaatgcg cctgctggtc agcgcgtcga acacgtccgc
     4201 cagcgccatg atgcgtgcgc tgatcggtat cgcttgtccc ttcagttggt ccgggtagcc
     4261 gttgccatcc cagcgctcgt ggtgccacat ggcaatctgg cgcgccacat ccagaaaggc
     4321 caatgggtaa gcatccacag ggtagattga ccgatccgcg tccctcaccc tttgaatcgc
     4381 cagcgcaaag gcatcaccgc caatctggct gtgggtcttg atcacttcaa actctgtcgc
     4441 ggtcagcctg cctggcttgc gcaaaatgtg atcggggatg ccaatcttgc caatgtcatg
     4501 caagggcgcg gcacgcacga tcagtttcag ttgctcatcc gacaacaatc gctgataggc
     4561 cggtcgctcg caaatggaac gtgctaacac ctcgacatag gcctgggtgc gatgaatatg
     4621 gtggccagtc tcaacatcgc gtgtttctgc caggatcgcc aaggcgtgca gactggcatc
     4681 ttgaaccagc tcattgtcat gcatgcgccg cagcacttct ttttccaaaa atgcgttttg
     4741 atctgccagc cagtcccgcg ccagcttgtt ttcaagctgc gctttgaccc gcgccagcgc
     4801 caatcgcggt ctgatcggct tggcaatgta gtcggcagcg caaagctcaa gccctttttc
     4861 ttcctcagat gcagcatcgc gagcggtcac aaaaatcaca ggaatatgca gtgttagcgg
     4921 gttgtcgcgc agccgggcca gcacgtcgta gccgtccaga tcaggcatca tcacatccag
     4981 caaaatcaag tccggaggcg gcagagacac cgctgcccgc aacgcccgat cacccgagtt
     5041 ggccaccttg acccgataga agggctcgag caactcaccc aaaatagcaa ggttctcggg
     5101 ttgatcatca actaccaata cgacaggggg cgctttcatt tttggtcatt cagcaaggca
     5161 agtgcctctt caaacaaaaa ctgctccagc attttgccaa ggtttgcggc agccacaagc
     5221 cccaaaatat cggtcaattc ttgccggtgc tgttcaaaaa atcgccgtgc acctatgtca
     5281 tctgcttcca gcagcgctcg caagctggcg acaacgcgca gcaggtcggc atcgctcttt
     5341 gccgtaggct ggtttggcat cgggatcagg gccgtcgtcg gcatcgcggc ctgaactgct
     5401 gcgatgaaaa cgggcagttc tatggcaagc agatcgagca agccggtcaa atcattgcgg
     5461 gactgttgtt tgatggctcg ctcaagcgct gctgcaagct gaccgatggc ttgtgcaccc
     5521 acgttgcctg cgctgccctt caaggtatgc gccgcacgct ctgccgcaga aaagtcctgg
     5581 cgatcaatga ggctttgcag cgtagttgct gcgtcggcat gcccctcgac aaacaggccc
     5641 aagacgcgcc aatacgaagg cagcttgccg cgcaccagat gcaatgccgc atcaacatca
     5701 aggccgggta tggcgcgcag cgcctgcaag gctggtgcat cgcgggtttc ctgcaggctg
     5761 gcaactggtg gtactgaaac ctggtcggca cctgaagtcg atggtaacca gcgcagcagc
     5821 gccttgacca gcgcgtcagg gtcaatcggc ttggccacat ggtcattcat gccgaccgac
     5881 aggcaggcct ggcgatcatc gtcaaacaca ttggccgtca tggccaaaat cggcacctgc
     5941 tgacgctctg gcaggcgacg gatgtcgcgc gtggcatcca gaccatcttt gcgtggcatc
     6001 tgtatgtcca tcagaatcag gtcgtaactg atgtcctttg ccttgttgac ggcaatctcg
     6061 ccgtcttggg ccaggtcaac cgccagaccc atttctgcca acaggtcaag ggccaccgcc
     6121 tggttaatcg gattgtcttc agccagcaaa acacgtcgcc ccttgaattg cgcctggtcc
     6181 agcctgatgc cagccgaagg caggtggcgg tgggcgcact taccagtcaa cacctcggtc
     6241 aaggcatcgt acaggctcga tgccgttacc ggcttatgca tcatgcagac caccacgccc
     6301 agccgcaaca actcggtggg ctgctcggca ccatgtccga gcatcaagat gacctttggc
     6361 gcaacctggt cggccgactc gcgaataccc tccacggcct ggttactgct catgtcgggc
     6421 aactgggcag ccaccagcgc caagtcaaaa ggcatgccaa cagccacggc ctgtgcgaca
     6481 cccgcaatgg cggccgcgcc actgctgaca accgtaggct ccatgccaaa gcgctgcagc
     6541 aagcgcatca gcacatcggt gctttccggg tggtcatcga ctaccaggat gcgccgcgta
     6601 tggtcgccga caatcgatgg aactggcacc agttcaactt cggattcaac cggttgcaac
     6661 gcaaaaagcg cctccagact gaaagtgctg cccctgccaa attcacttct tgcctggaca
     6721 ctgccaccca tcatctctgc cagtcggcgg catattgcca agcccaggcc ggtgccacca
     6781 aatttgcgtg tggtcgaagc atcgccttgt tcaaacgcct gaaacaagcg ggcctgctgt
     6841 tcggtcgaca agccgatgcc actgtcagtc acctcgaacc gaatagcgca ctgggtcagg
     6901 tcagccgaca gacacacagc agacaaaatc acatgcccat gcgccgtgaa tttgacagca
     6961 ttggtggcaa agttgatcaa aatttgcccg agtcgcatgc ggtcgccgat caggtggcga
     7021 ggcaaatcgg gatcaatgtc aagcaccact tccaatcctt tgctggcggc ttggtcgcca
     7081 atcatgtcgt acaccgttct aaacagggct tcaagctcaa aatcagccga ttccagggtc
     7141 agcttgcccg cctcgatttt ggaaaagtcg agaatgtcgt tgatgacacc gagcaaatgc
     7201 cgtgccgact cggtgacttt gtcgagttgc ccgatctgcc gctgcaccac ggcatctcgg
     7261 cgcaacaagt atgtcaagcc aataatcgcg ttcagtggtg tgcgaatctc gtggctcatg
     7321 ttggccaaaa aagcactctt ggcgcgattg gcttcttcgg ccaccacttt tgcttccgcc
     7381 agttcggcgg tgcgcgcttg caccagctct tcgagatggt cccggtgccg catcaactca
     7441 tcctgggtac tgcggcgctc ggtgatgtca tgggccatgc ccagcacgcc gatcaagctg
     7501 ccatcggtcg ccagcatggg cgtcttggtc gtctcaaaca gtccacggta accatcggcc
     7561 ttgaaagtga gccactcttc gttgatgctc gggctgcccg cttgcgccgc caggcgatca
     7621 ttcagacgaa agaactctgc agtttgccgg tccacaaagt catagtctgt cttgcccaga
     7681 atctcggact cttttgcacc atataagcgc tcaaagggtg gattgcaggc cagataaacc
     7741 ccttgtgcgt ccttgagcca gatcagcgcc gggatggtgg ctaccagggt gcgcatctgc
     7801 gcctgctcga actccagggt gtcgagcgta tggcgcagat tgttctggga tttgagcaac
     7861 tgacgctcaa tgcgaccgat caaataaaac atcaccgcag cgaacaatgc caaagcaaaa
     7921 aatgatgcca gcgacaccag caccagcaag cggctggttt gcctgagttg ctggtcgctc
     7981 tgacgacgaa cacggtcatt gagttgcttg aggtcatcaa gcaggaagtt ctggcgctgt
     8041 tgcaagaaat cgatcgaatt caccaaggtg cgcaccgcca cgtaatattc accggtttca
     8101 ttggcgttca gaccttcgcc caagcggcgc agcagggact tttccatcag actgccctcg
     8161 ctgaacaccg ggctcatgaa ctgacgactc atctcgtcaa tttcaatgcg tatggacttc
     8221 caggccttgt caatggcaag catttctacc gcaaacgctt gcctcagttc tggcaacttg
     8281 ccttcaaact catcgacaga ggccacccac tggtggcgct gcttggccaa ttcgggggtg
     8341 gaaagcaggc gctcggtatg gtatttgagc gccaacaccg acttgtaaga gtcatccacg
     8401 gcccgttgac gggccacaat ggcgctgtag tcgaagatca ggtaagccac cgtgatgccg
     8461 ctgatcaggg cacccacaat cagcaaaccg aacccataca aagacagggt tcgaatgctc
     8521 agttgcgcca gtcgcacggt gcgcgccgcg ttatttaccc acgttcagcg atgcctggta
     8581 aaaagcgatc tgcttgtcac ggcccaggcg gtcggtgatt ttttcccact gcgccgccgc
     8641 ctgatcaagc gccttggccg gtgacaactc accgctgacg gccttgtaaa catactcgtc
     8701 aaacacctgg taatactcac tggcacccgg aatagtgatg tcatacacca cgttcgggtt
     8761 ggacagcgat ttggtgattt cctccagata ggctttggca gactcagtcg aaaagcccga
     8821 ctggttccag gcctcgggac gatcaaaatg cgacttgcgc gacggattga cggcggtgcc
     8881 gctcattgcc gtcattttct tggtcagttc cggggaggtc atgtgcgagg caaactcaaa
     8941 agccagtgcc ttgttcttgg aagccttgct aacaaaaaag gtccagttgc cgctgatcga
     9001 agacacctga ttaggccgct ggtcccactt gcccgtcaga ctgttgaaca cttccgcgct
     9061 accgggaatc tgggcgtagc cgattttgtc cttgatcacc gacgaaggcg cgttgacagc
     9121 gtgcacaccc atatcggccc agtcaatcgc catcgccgtc tgaccattga caaagctatt
     9181 gcgcacatcg tggccggcaa aattggccat gcccggtgga ccaaacttca gggtgtcaat
     9241 ccagatgccc agcgctcgcg caaagccggg gttgttgacg cggggtttca gggtgttctg
     9301 atcaaaataa taggccgggt catccgggtg tttcgcgtgg gcagcggcct gggcaaaaaa
     9361 ctgcagcatc gatatgtcgc ccttgacccg gtttccggcg ataccgtagt caggcttgcc
     9421 gtcaccactc cagtcccagc ccgtaaagaa ctcggccacg tcacggtagg ctttccagtt
     9481 ggccgggggc gccagatcgt atttgtattt tttcttgaag cgtgcctggt agtccggcct
     9541 ggcaaacaaa tctttgcggt aatacagatt gatgcaatcg ccgtcgtagg gcaaaccgta
     9601 ggccacgttg ttccaggaca gcacggtgtt gcggtagatc ggcagcacgt cgtcccagtc
     9661 aattttcttc ttgatggcat cgggcaccgg ctcaatgtgg ccaccaccga gcaatgtgcc
     9721 gctccacatg gaaatgacca cgtaaatgtc aaagctggca tcgggcgatt tgatcgattg
     9781 gtcgatcttc tcgatggtct cgttccatcc tggtgtcagc acccgcacct tggctccggt
     9841 gcgggcctcg aacccctcga cttcatccag gataggtcca cgaatcagcc gggcatcaag
     9901 cgtgggcaca ataaccgtaa tcgactggcc ttcaaacggt ctgatggctg gtttggcggg
     9961 cggttccgca gctttgtcac cgcaacccga taccaatagc aaactcaatg cggccagtac
    10021 gtacagtttc acgatgaact cctgtcaaat ctctgggaca tcaggaaaag ccaaccattt
    10081 cggtgggcaa tttacagtgt aggccgcaag ctgccgcttt gatattgatc tgcaacaagc
    10141 aaaacgctga ttctgcccgg tgacgtgcag cctgtgacgc gaccaagtct ggcaagcagc
    10201 gcaccaacca tgggggtcgc cgaatcccgc tttgggcctg cgcctggcga tcctgtcact
    10261 ccgccacgga acttgatgcc gagggctgtc gtcattaggc cttcaacgcc gccagcgaga
    10321 gcatttttgg tggccatgat cgctttcgcg tccacgccga tagccgcact gaccggcctg
    10381 gtacgggtga gtttgcagat gcccatcagg gacagctatc agcccgacac cagccctgca
    10441 tgaaaacccg ctgccaaagc acgcttcgag tttgacttgc ctaagggtta gacctagtgg
    10501 tgaaacatct caggtgcact accctcgggc acccgttatt cattattgga cggcttagtg
    10561 acaacacatt ccaacacgag gcaaacatga cattgaaatc cattctggcc gctgcattgc
    10621 tggccgtcag cttgggcaca agcgcccaaa ccatcatcaa attcagccat gtcgtagcgg
    10681 ctgacacccc caaaggcaag gcctcggtgt ttttcgccca aaaggcggcc gagctgacca
    10741 aaggcaaagt caaggtcgag gtctacgcca acagcgccct ttacaaggac aaggaagaga
    10801 tggaagccct gcagattggc tcggttcaga tgctcgcccc gtcgttggcc aagtttggcc
    10861 cgctgggcgt gaaagagttt gaagcgttcg atttgccctt catttttgac gacaccgccg
    10921 acctgcacaa ggtcacccaa ggtccggtcg gcgccgcctt gatggccaag ctggagccac
    10981 gtggcatcaa gggtctggcc tattgggaca acggcttcaa gtcgttttcg gccaacgccc
    11041 cgttgaaagc ggttgccgat tacaagggca aaaagttccg cattcagtcc tccaaggtgc
    11101 ttgaagagca aatccgcgcg attggcggca tcgcccaggt gatggccttc tcagaggtct
    11161 atcaggcgct gcaaaccggc gtggtggatg gcaccgaaaa cccgatttcg aacttttaca
    11221 cgcaaaaaat gcacgaagtg caaaagcacc ttaccctgac caaccacggc tacctcggct
    11281 acgcggtgat tgtcaacaag aagttctggg atgccctgcc cgccgatgtg cgcggccagt
    11341 tggagcaggc gatgaaggaa tcgaccgtct atgccaacaa gattgcccaa gaagaaaacg
    11401 acctgtcgct cgacggtgtc aagaagagcg gcaagacggc ggtgtatgtg ccgaccaagg
    11461 aagaacgcct ggctctgaag aagatcatgg ccccggtgca tgccaagatg gcggaccggg
    11521 ttggcaagga aaccctgcaa gagatctaca aggccaccgg ttttgacccc aacacgctgt
    11581 aaccccgtct gacaacaagc tgctgcaata aagagtcccg gacgggtctg ttttgatgaa
    11641 atttcttgat cacctggaag agtggctggt caccttcctg atgggcggcg ctacgctgat
    11701 catctttgtt gcggtgctgc accgctacgc tgccggcatg gccatacccg gcgtgcagga
    11761 ctggctgttg tcgatcagca tgagctgggc gcaagagctg accatcatca tgtttgtctg
    11821 gatgtgcaag tttggtgccg cctacggcgt gcgcaccggc attcacgtcg gcgtcgatgt
    11881 gctgatcaac cggctgaaca agcaaaaccg tggcaaattt gtcatctttg gcctatgcgc
    11941 cggtgtcttg tttaccggca ttgtggccgt cattgcgtcc ggttttgtct gggaaaacgg
    12001 tgcccaccac ttctggctca atctgttggg ccggtcggtt gaaggcgttc ccgaaggccc
    12061 gaccacgcca gacctggagt ggccgacctg gatggtctat agcgcgatac ctctgggcac
    12121 cagcttgatg tgtttccgct ttatccaggt gctggtgatg ttcgtcaaaa ccggtgactt
    12181 gccgcaccac gaccacggcc atgtggatgg actcgaagtc gatccggatg acccgctgga
    12241 caacccctac gccatggccg acaacctgca cccgcgtgac ctgcaccatt cgctggaagg
    12301 caaaacccgt catcacgacg cgaccgacgg gagccaatcc aaatgaatgc cgccatcatt
    12361 ttctttctgc tgatggtgct gatgctcacc ggcatgccga tctcgatctc gctgggtctg
    12421 acagtgctga ccttcctgtt tggctttacc aatgtgccgc tggagtcggt ggcgctcaag
    12481 ctgtttaccg gcattgaaaa attcgagatc atggcgattc cgtttttcat tctggcgggc
    12541 aactttctga cacatggcgg tgtggcgcga cgcatgatca actttgcgtc gagcatggtc
    12601 ggccattggt atggtggtct gg
//
LOCUS       DAOSLL010000052        12578 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5050_length_12578_cov_5.108840, whole genome shotgun sequence.
ACCESSION   DAOSLL010000052 DAOSLL010000000
VERSION     DAOSLL010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 12578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12578
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5050_length_12578_cov_5.108840"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            570..4844
                     /locus_tag="PLB25_03680"
     CDS             570..4844
                     /locus_tag="PLB25_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40720.1"
                     /translation="MNTTSRNVTPIELTRTFFATPDMQDDGEDWSDYLGSAKGRLTWE
                     DLRDKPLAVVLGQAGIGKTVEFELEVQRLRSAGKAAFFVALNQLVDSESWERALAGSY
                     SSYEQWKASNETAYFFLDAVDEARIKGQADFVQALSVVPWMLRECMSRVRFVISSRPT
                     DWSIDGVRAAVDKYLGKPIAAAPSVLNEAPASAIGTFGTTVNVVRLPAADAIEPLVAS
                     LEPLSISEAKRLAEAFGVQEAKAFWDTVSDGSYAFMATRPLDLGWMVRLWNEKRSLGT
                     YRELIEFNIENRLTEVNPSYLAAGAVLSQDQLRMGSEELAAAAELSGRAYIATEYAPP
                     VRADEVASSSVLSGWRPNEVARLLSAAVFDEATFGRVRFHHRTTRAYLAACWVNRQLV
                     IGVPFHRVLPLFAASPFGTAVLIPSRRWALCWLAAINVEAREWLTRHFPEMLLFDGDP
                     EAWDSLSADRAFMAYVQRLKSGLRTDWYNDQSEFRRVGRTLSPGLVAGLLATPELPVR
                     VTTALLPIAKHGRLTDCAEVVFSIYKNIASSQCERRYALEVLETIATPAQRVAIKADL
                     VSGLLTSNALIASALPTTDWQGLSIAQLVAIFMRTTSEEHYGGGSMAQTLKEDLLPVA
                     TATSAEMLLRAVLEALPRPEARKRFARFSDSDQPERAWLLDVLPDCFERLLTLLPRTD
                     GDYPDSCIEAAERLEALRDSGFIDREEFGRIHILVAEHSGLRWQIALEIALSENILHS
                     TSRLTWGRNCLVNFEAADIPELILRANDTAVSQDVRNIWFTVGMEVTFRGLRGHTRSG
                     ALAALAADHDCEARKTRIATQRANLVNGAKQRREWRAGEAVRKREQQIQHEVSRTQLL
                     GDAGHLRDASHKGSLHWLIHYSYERAGHKSFTHVDYELISRDFGQTVADALASGLKVV
                     WADAEIPNPADYKDGAVPWEALTALAGLHTLLDEGVNIASLTDADASRAAKLAVWELN
                     GPPSWFDPLAKAHGPVVSEALRPWIESEAELTTDAHHLRGGLEMALRCSTAVRAALLV
                     PLVPMLTDGRIGCPETFKELVKALRAGGLIAAHVLSELCRSRVVTSISPEGLLGETYW
                     LHTWLEEDPASAWGWFEQHVASSPAVAGELVKAFAKMAQDCKWVKQPADAASIDVLLR
                     LHSLLTQSQPSPGAPVDPEDAGTFGHVVTELRSRIPNVLVQSRGAAAHRALVHLMEAE
                     AAPNTKQWLAAQVAEHAAREASESSQVEPCDLSDIGSPFVAAPKNEAQLFVQVMARLE
                     EVRKGTEEGPFSDRALFRPGMPEAHLQSWLAARFSETKNRRFTVTREEQVDNDKEPDI
                     QLGCPQGKVCVEVKPLDKGRGYSAKSLTETLRVQIVKQYLRGFNSNHGILVLFKLDYK
                     TWTIPGGKTKQPFSALVEYLQSQADIIKNESPDVIALQIVGINCVI"
     gene            6461..7618
                     /locus_tag="PLB25_03685"
                     /pseudo
     CDS             6461..7618
                     /locus_tag="PLB25_03685"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
     gene            7640..8893
                     /gene="glgC"
                     /locus_tag="PLB25_03690"
     CDS             7640..8893
                     /gene="glgC"
                     /locus_tag="PLB25_03690"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HOE40721.1"
                     /translation="MLHVAQKTYGLILAGGRGTRLDQLTAERSKPAVPFGGKLRIIDF
                     PLSNCVNSGIRRVAVLTQYKAQSLIRHIERGWGFLEPTLSEYIDVVPAQQQINAGWYS
                     GTANAVFQNLALLDEADPTYVLILAGDHIYKMDYTRLLADHVNSAADLTVACIELPLQ
                     DVTGFGVMAVDEGARVTAFEEKPTNPIPIPDNPLYALASMGIYVFNAAFLREQLRQDA
                     ADEHSSHDFGRDLIPRLIHSHHVQAHRFTDSCVNMIEGRRPYWRDVGTVDAYWEANMD
                     LTHVVPDLNLYDDDWPILSVHERSAPAKFVFREPALRGMALDSLVSNGCIVSGANVYG
                     SVLFTKVRIAEYSQIEDSVLLPEVVVGRNVIIRRAVVDSGCIIPDGFQVGINPDSDRA
                     RFYVTPRGIALITPEMLGQPPHRMV"
     gene            complement(9457..12555)
                     /locus_tag="PLB25_03695"
     CDS             complement(9457..12555)
                     /locus_tag="PLB25_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOE40722.1"
                     /translation="MNVSAWSIRNPIPAVMLFVLLCFAGMLSFNAMKVQQFPDLDLPT
                     VTVSASLPGAAPAQLETEVARKIENAIASLQGLKNIYTKVQDGGVTVTAEFRLEKPTQ
                     EAVDDVRSAVQGVRSDLPTDVRDPLVRKMDLAGAPILAYAVRVPSMDDEALSWFVDNT
                     LTRRLLSVKGVGAISRVGGVTRQVEVALDPFKLQALGATAADISRQLRQVQTESAGGR
                     ADIGGSEQPMRTLATVKTVAELSQLELALSDGRRLRLDQVASVQDTVAEPRALALLNG
                     VPVVGFEVSRSRGASEVEVGAAVRLALDELKQQHPDMVLTEAFDFVTPVAEEYDGSMV
                     LLYEGAILAVVVVWLFLRNWRATFVSAVALPLSVIPAFIGMGYLGFSVNVITLLALSL
                     VIGVLVDDAIVEVENITRHLHMGKTPYQAAMEAADEIGLAVIATTFTLISVFLPTAFM
                     AGIPGKFFKQFGWTAALAVLASLVVARALTPMMAAYVMKPDARQHQQPWWMARYLRWS
                     DWALSHRWVTMAAAGAFFVGSLMLIPLLPKGFIPPDDNSQTQINLELPPGATLAQTRA
                     AAETARLLVTQLATVQSVYTTIGGGAAGSDPFAPQGASEVRKATLTVLLTERGKRPRK
                     QGIENEIRAALAELPGVRSKVGLGGSGEKYLLVLTGEDPLALATAATAVEKDLRTIPG
                     LGQIASSASLIRTEIAVRPDFARAADLGVTSSAIGETLRIATVGDFDAGLAKLNLSQR
                     QVPIVVKLADAARQDLDLLGRLAVPGLKGPVMLSQVASLSLTGGPAVIDRLNRSRNIT
                     FEIELSGAPLGDVTEAVAKLPSITSLPPGVKQLALGDAEVMDELFASFGLAMLTGILC
                     IYIVLVLLFKDFLHPFTILTALPLSLGGAFVALLGTGMSFSMPSLIGLIMLMGIATKN
                     SILLVEYAIVARRGHDGSDGHPVVAPMSRHDALIDACHKRARPVIMTTLAMGAGMLPI
                     AVGWGAADSSFRSPMAVAVIGGLLTSTVLSLLVIPAVFTVVDDFGGWMRRRLGLGGVA
                     QV"
BASE COUNT         2702 a         3714 c         3685 g         2477 t
ORIGIN      
        1 agatgccggt cagcatggcc aggccaaagc tggcaaacag ctcgtccatc acctccggca
       61 acgcccagcg ccatttgatt cacattgggt ggcaggggtg ctgcaaaaag agctagcttc
      121 aactcgggcg acaccggttc gcaagtattc agccaatgtc attggcagtt atgcagcggt
      181 tgcggtcagt cgaatcaaaa ttgggagtat cgggtttgga ccagttgcag tcggtcgtca
      241 atggccggtc tggcgtctct gaaatttgcc tcaacaagat gccagaaacc ggaccttcgc
      301 caacaaccgc atagtgccca ttgcagtcgg tcgctggtcg gcgcataggt gactgcaatg
      361 gaaatcgaac ctgccgttca gtttcttcca tcaataggtg gcccgaccag aggccgtctg
      421 ccgccaaaaa cgatcaattg tggctatcag gaatggaaca aatgccgcga ctaccaggcg
      481 gcttgtccgg atgaattgaa ttggttcgga aagagccgca ctatgtatgg ctagagagcc
      541 gaccatcaac ctcggcgaaa atcgccgcaa tgaacacaac ttctcgaaat gtgacgccaa
      601 tcgaactaac ccgaacattt ttcgctacgc cagacatgca ggatgatggc gaagactgga
      661 gtgactacct cggctcggcg aagggaaggc ttacctggga agacctgcga gacaagcccc
      721 ttgctgtggt tcttggccaa gcaggcattg ggaaaacggt tgagttcgaa cttgaagtcc
      781 aaaggcttag aagcgcaggc aaggcggcgt tcttcgtcgc gttgaatcag ttggtcgatt
      841 cggaatcctg ggaacgtgcg ctggcgggct catacagtag ctatgagcag tggaaagcgt
      901 cgaacgaaac ggcgtacttt tttcttgacg cagtggacga ggcacgcatc aaaggacagg
      961 ccgactttgt gcaggcacta tccgtggtgc cgtggatgtt gcgcgagtgc atgtcccgag
     1021 tgcgcttcgt catctcttcc cgcccgacag actggtcaat cgacggtgtc agagcggccg
     1081 ttgataaata cctcggcaag ccaatcgcag ccgccccttc cgtattgaac gaggctccgg
     1141 catccgcaat tggcacgttc ggcacgacgg tcaacgtcgt gcggttgcca gctgcggatg
     1201 ccatcgagcc gcttgtcgcc tcccttgagc cactctccat ttcggaagcg aagagacttg
     1261 ctgaagcatt tggcgttcag gaagccaagg ccttctggga tacggtctct gatggcagct
     1321 atgcattcat ggccactcgc ccccttgatc tgggatggat ggttcgccta tggaatgaga
     1381 agcgcagcct gggaacctac cgtgaactca tcgagttcaa catcgagaac cggttgaccg
     1441 aggtgaatcc aagctacctc gccgcaggtg ccgtgctctc tcaggatcag ttgcgcatgg
     1501 gttcggaaga gcttgcagcc gctgcggagc tttcaggccg cgcctacatc gcaactgaat
     1561 atgcgccacc ggtcagggcc gatgaagttg cgtcgtcgtc tgtgctctca ggatggagac
     1621 caaacgaagt cgcgcgactg ctttccgccg cagttttcga tgaagcaacc tttgggaggg
     1681 ttcggtttca ccaccggacc acacgagcgt acctggcagc gtgttgggtg aaccgccagc
     1741 tcgtcatcgg ggttccgttt catcgtgtgc ttcctctctt tgccgcgtca cccttcggca
     1801 ccgccgtgct tattcctagc cgccgctggg cgctttgctg gcttgcggcc atcaatgtcg
     1861 aggcacgaga gtggctaacc cggcactttc cagaaatgct cttgtttgac ggtgacccag
     1921 aggcctggga ctctctgtcc gcagatcgcg ccttcatggc ctatgtccaa cgactgaaga
     1981 gcggactcag aacggactgg tacaacgatc aaagcgagtt tcgccgcgta ggccggacgc
     2041 tgtcgccggg cctcgttgct ggcctgctgg cgacgcctga gctgcccgtc cgggttacta
     2101 cagcgctgct gcccattgcg aagcacggtc gcctgacgga ttgcgccgag gtagtgttct
     2161 ccatctacaa gaacattgca tcgagccagt gcgagcgtcg atatgcgctt gaggtcctgg
     2221 agacgatagc gacgcccgcg cagcgcgtag ccatcaaagc ggaccttgtt tcgggccttc
     2281 tcacgtccaa cgcactcatc gcatcggcac tgcccaccac cgattggcag gggctgagca
     2341 tcgcgcaact ggtcgcaatc ttcatgcgaa ccacgtccga ggaacactac ggaggagggt
     2401 ccatggcaca gaccctcaag gaagacctgc ttccggtggc taccgctacc tcggcggaga
     2461 tgctgttgcg tgccgtgctg gaggcgctcc cgaggccgga agcgaggaag cgattcgcac
     2521 gattctcgga ctcagaccag cccgaacggg catggctttt ggatgtactg ccggattgct
     2581 tcgagcgctt gctcacgtta ctgccccgga cggacggtga ctatcccgac agctgcatag
     2641 aagcggccga acggcttgag gcgttacgtg acagcggatt catcgaccgc gaggagttcg
     2701 gacgcattca catccttgtt gccgagcact cagggcttcg ctggcagatt gcactggaga
     2761 ttgcgctgtc cgaaaacatc ctccattcga ccagtcggct cacctgggga aggaattgcc
     2821 ttgtgaattt tgaagccgcc gatattcctg aattgatact gcgggcgaac gatacggccg
     2881 tgagccaaga cgtgcgcaat atttggttca cagttggcat ggaggtgacc ttccgaggtc
     2941 tcaggggcca tactcgcagc ggcgctctgg cggcccttgc tgcggatcat gactgcgagg
     3001 cgcgaaagac acgcatagcc acacagcgcg ctaacttggt gaacggcgcg aagcagcgtc
     3061 gtgaatggcg ggccggagag gccgttcgaa agcgcgagca acaaattcag catgaagtca
     3121 gcaggactca acttttgggt gatgctgggc atctccgaga cgcgagtcac aaggggtcgc
     3181 ttcactggct gattcactac tcctacgagc gcgcgggcca caagagcttc acgcatgtcg
     3241 actatgaact aatttcgagg gattttggtc aaactgtcgc agatgcgctc gcctccggcc
     3301 tcaaggtggt gtgggctgat gctgaaatcc cgaacccagc ggattacaag gatggtgcgg
     3361 tgccttggga ggctcttacg gcgttggccg gcttgcacac gctgctggac gagggcgtaa
     3421 atatcgcgtc gctgacggac gcagatgcaa gcagggcagc caaactggct gtgtgggaac
     3481 tgaacggacc accgagctgg ttcgatccgc tggccaaagc gcatgggccc gtggtttcag
     3541 aagcccttcg accatggatt gagagcgaag ctgagcttac gaccgatgcg catcatttgc
     3601 ggggcgggct ggagatggca cttcgatgct ccacggcggt tcgtgctgcg ctactggttc
     3661 ccttggttcc gatgctcacg gatggacgca tcgggtgccc agaaacattt aaggagcttg
     3721 tcaaggccct tcgtgcgggt ggactcattg ccgcccacgt tctctccgag ctctgtcgtt
     3781 cccgagttgt cacctcaata tcgccagagg gtttgcttgg cgagacgtat tggctgcaca
     3841 cctggctgga agaagatcct gcgtccgcat ggggctggtt cgagcaacac gtcgccagtt
     3901 cgcccgctgt agcaggcgag ctcgtgaagg cgtttgccaa gatggcccaa gactgtaaat
     3961 gggtcaagca acccgccgat gcagcttcga ttgacgttct tcttcggctc catagccttc
     4021 ttacccagtc ccagccgtca cctggcgcgc cggttgaccc cgaggacgcg ggaacgttcg
     4081 gtcatgtcgt taccgagctt cgctcaagaa tcccgaacgt actcgtccaa tcaagagggg
     4141 ctgcggcaca ccgagccctg gttcatctga tggaggcaga ggctgccccc aacacgaagc
     4201 agtggcttgc agctcaagtt gccgagcacg ccgcgcgtga ggcctcggag tcttcccaag
     4261 tcgagccatg tgacctcagc gatattggct cgccgttcgt cgcagccccg aagaacgaag
     4321 cccaactctt cgtgcaggta atggcccggt tggaggaggt gcgcaaaggc actgaggagg
     4381 gcccgttcag tgaccgagcc ctgttcaggc cgggtatgcc ggaggcgcat ctacagagtt
     4441 ggctagcggc gcgcttctcg gaaacgaaaa atcggcggtt cactgtcact cgcgaggagc
     4501 aggtggacaa cgacaaggag ccggatatcc agctcggttg cccgcaaggt aaggtctgtg
     4561 tcgaagtaaa gccgttggac aaaggccgtg gctactctgc gaagtcactg accgaaacgt
     4621 tacgagtgca aatcgtgaaa cagtacctga ggggattcaa cagtaaccac ggcatcctag
     4681 tgcttttcaa actggactac aagacatgga ccattcccgg cggcaaaacg aagcagccgt
     4741 tctctgcgct cgttgagtat ttgcagtcgc aggccgatat cataaaaaat gagtcacctg
     4801 acgtgattgc tcttcagatc gttggtatca attgtgtgat ttgagattgc tgattcctca
     4861 aatctgcgga aacttcgtct ttcgtgggca gagaaatatc tcgttcggtc actgcgggcc
     4921 tgcggtagtg ggcatgtatc gttcaatgca cagcaaatga aggtagccgc attgccatgg
     4981 tgaatgacag gtgctagaga agctgcagtc gctcatctcc atgctagtga gtgactcctg
     5041 atggccaaac gcgggtgccc gatttcacga tggggtcacc tgatagctga cagtcgcttt
     5101 gtatcagcca acggccggat tggaggagtc agatcggaaa cggctgctac cggcaagggt
     5161 ctcaagaagc cccgcaacct taacagtaaa caagccgctt taagctataa atcaaagagc
     5221 tatcagcgca tatggaaagc ggtctacagg ccattttgac atgtagatgt cacgcactca
     5281 catgatctct gccagcgcca tccgtcaagc gcccgttggc gtacagctta cggtgttcaa
     5341 gctccccgtg cgcacaatca acagcaccgg cgcagcagcc agacacaggg cggccccgat
     5401 ttctggtact ccagtatgag gggccgccct gtctggctgc tgcgcagcac cgaagcgtcg
     5461 caccaccagg acaccagccg cacaaccccg attggcgcgg catgaatcgc ctgaaaaaac
     5521 aagatgacta aaaggccaat gaatctgagc ggctggctgt gatgtgatgg gatgtgatag
     5581 tccgcacgac tggcagtcag cttagcgcga ctttcaacca ggcagcaatg tgagaacgtc
     5641 gcgagcgaat gaaaaacaat agctgccagc gcttatcctg tatgcgccag gagccaattt
     5701 gatgcctaag cctgcgccac cccgccgagc cacggccgcc agagcggaca ttatcgatgt
     5761 ggggtcagtt tcgtcagccg accatcgtta ctacccgaaa gcagtcagtt gatggcggtg
     5821 agccgttctt ccaaatggcg gtcgaccacg cagcgacagg atccattggg ggtttggctg
     5881 tgaatgactc caatatgcta aaaagcggca gtagcggttg gcgatttgcc tgttccaaac
     5941 cagtcgttga taccgtcgta cgactgtatg ctggcctacg ccatgggagg acggtgatgc
     6001 gcaggtggtt gaggagacat caatcgactc ggattgggat cttgcagagc aaccgaccac
     6061 cgactactgt gtcgatcagc gcatcaactg gtgactggtt gaggcggcga ctctgacttg
     6121 ctgctgggta gctccgcgtc tggcgcaccc ggtgccccag aaatcacttt tcatgacgtc
     6181 gcttttggtg gtaaacctgc caaggaatct agcaagccga gcggattcag gcggttcaag
     6241 acctgctcca gttccctgat gcgatacttg gcaccgtgtg cttgaaatgc ttatcccttg
     6301 cacatacaac cgtcatccat tggcaaggca cggctgaaaa cgacttgcac ttttacattg
     6361 cgctattcct atgactgagc gagatgcctt ctctgcccta tttgcttcag aaccggccaa
     6421 caacgcgatg ccactggtgg ttgatcgacc ttggaaagtg atgctggtcg atgatgaaga
     6481 tgatatccgt gccgcgctgc gccttgcgct ggtggatgtg cgggtaggtg agcgaacctt
     6541 gcaattgctg gaagcaaaat cctgcgagga tgcgaaaact cgcctgactg agcatcccga
     6601 caccgcactg atcctgcttg acgtcgtgat ggaaaccaaa aacgccggac tggagctggt
     6661 gcgtcacatt cgcgaagaac tcggcaacca tcagacgcaa atcgtgcttg ttaccggcca
     6721 gcctggccac gtaacgcagc gcgaagtcat tacaagatat gccatcaatg gctaccatct
     6781 aaagtcagac ctgtcagccg acaaaatttt cagttctgtc cattcgggct tacgctcgta
     6841 tcaggcgctg gttgcgctgc aactgcaaca gcaacaactg tccaatatcc tctggggttc
     6901 cgggattggc acttgggaat ggaacataca taccggtgaa gccagattca atgaacgctg
     6961 ggccgaaatt cttggctatt ctcttgaaga gctggctccg gtcagcatcg acacctggat
     7021 gcgacgactg catccagatg acctggagag tttcaataac cgtcttccca actacttttc
     7081 cgacaaatcg gaccattacg aatgcgaagc ccgcgtccgt cacaagctag gtcactgggt
     7141 ctgggtactt gatcgcggca aggtggtgag ccggacaccc gatgggctgc ctgaatggat
     7201 ctccggcacc cgagacgaca tcagcttgcg caagctggca gaagaggcgt tggcggcaag
     7261 tgaagagcga tttcgcacag tcgccgaaca cacgcttgac tgggaatact gggaagggcc
     7321 ggaacatgaa atgttgtaca tgagtccgtc ttgcgaaaga ataactggct accaacggga
     7381 tgagttcttt gctgatccgt cgctgctgat gcacatcact cacccggatg acgtggcttt
     7441 gctgcaccac catcatctca acgtgagcca tgacgaagac ggttcactgg aataccgcat
     7501 cgtgcaccgc gacggcgcag tgcgctggat ggagcacgga tgtcacccgg tacacggtgt
     7561 caacggccag ttccgcgggc ggcgggtcag caaccgcgat gtcactgaac gtaagaaagc
     7621 agaagcacgc gccaccgaat tgctgcatgt ggcacaaaag acctacggcc taatactggc
     7681 cggtggccgt ggcacccggt tggatcagtt gacagccgag cgctccaagc cagccgttcc
     7741 attcgggggc aagctgcgaa tcattgactt ccctttgagc aactgcgtca attccggtat
     7801 tcggcgcgtc gcagtgctca cgcaatacaa agcgcagagt ttgattcgtc acatcgagcg
     7861 tggttggggg tttctggagc ccaccctgag cgaatacatt gacgtggtgc cagcacagca
     7921 gcaaatcaat gccggctggt acagcggcac ggccaatgcg gtattccaaa acctggccct
     7981 gctcgatgag gctgacccga cctatgtgct gattttggcc ggtgaccaca tctacaagat
     8041 ggattacacc cggctgctgg ccgaccatgt caacagcgca gccgacctga ctgtggcctg
     8101 catcgagttg ccgctgcagg acgtaaccgg ctttggtgtc atggcggtag acgagggcgc
     8161 acgggttacg gcctttgaag aaaagccgac caaccccatt cccattcccg acaatccact
     8221 ctacgcgctg gccagcatgg gcatctatgt gttcaatgcg gctttcctgc gcgagcaact
     8281 ccgccaggat gccgccgacg agcattcgag ccacgatttc ggacgcgatt tgatcccgcg
     8341 cctgattcac agccaccatg tgcaggcaca ccgttttacc gacagttgcg tcaacatgat
     8401 cgagggtcgc cgcccatact ggcgcgatgt tggtaccgtg gatgcctatt gggaagccaa
     8461 catggacctg acccatgtgg tacccgacct gaatctgtac gatgacgact ggcctatttt
     8521 gagcgtgcat gaacgctcgg caccggccaa attcgtgttt cgggagccag cgctgcgtgg
     8581 tatggcgctg gactcgctgg tgtcgaatgg ctgcattgtc agtggagcca acgtttacgg
     8641 ctcagtactg ttcaccaagg tacggattgc agaatacagc caaattgagg actcggtgct
     8701 gttaccggag gtggtcgtgg ggcgtaacgt gatcatcagg cgcgccgtgg tagacagtgg
     8761 ctgcatcatc ccggacggct ttcaggtcgg catcaatccc gattccgatc gtgcccgctt
     8821 ttacgtcaca ccgcgcggca tcgctctcat caccccggaa atgttgggcc agccgccaca
     8881 tcggatggtg tgagggcagt caagtggttg cgggttttcc gaagaccatc tcactgtaaa
     8941 ttactgcttc cgaatttcca acacccgatg cgaacgtctg caatcggcga aattgaaggt
     9001 cagctttcga ctacgactag tcgctagatt tcatgcggct acggtgtacg tcgtctttgg
     9061 gtctcaagaa gcccctcaac cttaacaaaa acaagtcgct ataagctata aatgaaagag
     9121 ctatcagcgc atatggaaag cggtctacag gccaatttga catgtaaatg tcacgcactc
     9181 agcgtgatcc ctgtctggct gctgcgcagc accgaagcgt cgcaccacca ggacaccagc
     9241 cgcacaaccc cgctcgcgcg gcatgaatcg cctggaaaaa caaaatgact aaaaggccaa
     9301 tgaatctgag cggctggatg tgatgtgata gttcgcacga ctggcagtca gcttagcgcg
     9361 actttcaacc aagtagaaat gtcagatcgt cgcacgagaa tcaaaaacaa tagctgccag
     9421 cgcttatcca gtatgcgcca ggagccaatt tgatgcctaa acctgcgcca ccccgccgag
     9481 ccccagccgc cgacgcatcc agcccccaaa atcatccacc acggtgaaaa ccgccggaat
     9541 caccagcaaa ctcaacacgg tcgatgtcag cagcccgccg atcacggcca ccgccatcgg
     9601 cgagcgaaag ctgctgtcgg cggcacccca gcccaccgca atcggcaaca tgcccgcacc
     9661 catggccagc gtggtcatga tgaccgggcg ggcgcgcttg tggcaggcgt caatcagcgc
     9721 gtcgtgccga ctcatcggtg ccaccaccgg atggccgtcg ctgccgtcgt gcccgcggcg
     9781 cgccacgatg gcgtactcca ccagcaaaat cgagtttttc gtggcaatgc ccatcagcat
     9841 gatcaggcca atgagtgagg gcatcgaaaa actcatgccg gtgcccagca gcgccacaaa
     9901 cgcgccgccc aaggacagcg gcagcgcagt caggatggtg aacgggtgca aaaagtcctt
     9961 gaacagcagc accagcacaa tgtagatgca caagatgccg gtcagcatgg ccaggccaaa
    10021 gctggcaaac agctcgtcca tcacctcggc gtcacccagc gccagttgtt tcacacccgg
    10081 cggcagcgac gtgattgacg gcagtttggc gactgcctcg gtcacatcgc ccagcggcgc
    10141 gcctgagagt tcaatctcaa aggtgatgtt gcgcgaacgg ttgagccggt cgattaccgc
    10201 cgggccgccg gtgagcgaca ggctggccac ctggctgagc atcaccggcc ctttgagccc
    10261 cggcaccgcc agccgtccca gcaagtccag gtcttgccgc gccgcatcgg ccagtttgac
    10321 cacaatcggt acctggcgct ggcttaaatt gagcttcgcc aggccagcat caaagtcccc
    10381 cacggtggcg atgcgcagtg tctcgccaat ggcgctgctg gtcactccca ggtcggcagc
    10441 gcgggcaaag tcggggcgca ccgcaatttc ggtacggatc aggctggcac tggatgcaat
    10501 ttgccccagg ccggggatgg tgcgcaggtc tttttcgacc gccgtggcgg ccgtggctag
    10561 cgccagcggg tcttcgccgg tcagcaccag caggtatttt tcgcccgaac cgcccaggcc
    10621 caccttgctg cgcacccctg gcaactcggc cagagcggca cggatttcgt tctcgatgcc
    10681 ctgcttgcgc gggcgcttgc cgcgctcggt caatagcacg gtgagcgttg ccttgcgcac
    10741 ctcagacgcg ccttgcgggg caaacgggtc agagccggcc gcgccgccac cgatggttgt
    10801 gtagacgctt tgcaccgtgg ccagttgggt caccagcagg cgcgcggtct cggccgcggc
    10861 acgggtttgc gccagtgtgg caccgggggg cagttccaga ttgatctggg tttgtgagtt
    10921 gtcgtcgggc ggaatgaaac ccttgggcag cagcggaatc agcatcagcg aaccaacaaa
    10981 gaacgcgccg gccgccgcca tggtgaccca gcggtggctc aaggcccagt cgctccagcg
    11041 caggtagcgc gccatccacc agggctgctg gtgctgccgg gcgtcgggtt tcatcacata
    11101 ggcggccatc atcggcgtca gggcacgcgc caccaccagc gaggccagca ccgccagcgc
    11161 cgcagtccag ccaaactgtt tgaaaaactt gcccggaatg ccggccatga aggcggtcgg
    11221 caaaaacacc gatatcagcg tgaaggtggt ggcgatcacc gccaggccaa tttcgtcggc
    11281 cgcttccatc gccgcctggt aaggtgtttt gcccatgtgc aggtggcgcg tgatgttttc
    11341 cacctccaca atggcatcgt ccaccagcac accaatcacc agggacagtg ccagcagcgt
    11401 gatcacgttg accgaaaagc ccaggtagcc catgccaata aaagccggaa tcaccgacag
    11461 cggcaaggcc accgccgaga caaaggtggc gcgccaattg cgcaaaaaca gccacaccac
    11521 caccaccgcc agaatcgcgc cctcgtacag cagcaccatc gaaccgtcgt actcctcggc
    11581 caccggggtg acaaagtcaa acgcctcggt caacaccatg tcggggtgct gttgcttgag
    11641 ttcgtccagc gcgagccgca ccgctgcacc cacctcgacc tcgctggcgc ctcggctgcg
    11701 cgacacctca aaacccacca ccggcacacc gttgagcaag gccagtgcac gcggctcggc
    11761 cacggtatcc tgcacgctgg ccacctggtc cagccgcagg cggcggccgt cgctcagggc
    11821 cagttccagt tgcgacagct ctgccacggt cttgacggtg gccagcgtgc gcatcggttg
    11881 ctcgctgccg ccaatgtcgg cacggccccc ggcgctctcc gtctgcacct gacgcaactg
    11941 gcgcgaaatg tcggcagcgg tggcgcccag cgcctgcagt ttgaacgggt ccagcgcgac
    12001 ttccacctgg cgcgtgacac cgcccacccg gctgatggcc ccgaccccct tgaccgacag
    12061 caggcggcgc gtcagggtgt tgtcgacaaa ccagctcagc gcctcgtcgt ccatgctcgg
    12121 cacccgcacg gcataggcca atatgggcgc accggccagg tccatcttgc gcaccaacgg
    12181 gtcgcgcacg tcggtgggca ggtcgctgcg tacgccttgc acggcagagc gcacgtcatc
    12241 gaccgcctcc tgggtgggtt tctccaggcg gaactcggcc gtcacggtga cgccgccgtc
    12301 ctgcaccttg gtgtaaatgt ttttcagacc ctgcaaggat gcgatggcgt tctcgatctt
    12361 gcgcgccact tcggtctcaa gctgcgccgg tgcagcgccg ggcagcgatg ccgacacggt
    12421 gacggtgggc aggtccagat cggggaactg ctgcaccttc atggcgttga acgacagcat
    12481 gccggcaaag cacagcaaca caaacagcat caccgccgga atcgggtttc ggatggacca
    12541 ggccgacacg ttcataaata aaggctccta atttacta
//
LOCUS       DAOSLL010000053        12260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5203_length_12260_cov_4.430643, whole genome shotgun sequence.
ACCESSION   DAOSLL010000053 DAOSLL010000000
VERSION     DAOSLL010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 12260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12260
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5203_length_12260_cov_4.430643"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..327)
                     /locus_tag="PLB25_03700"
     CDS             complement(169..327)
                     /locus_tag="PLB25_03700"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOE40723.1"
                     /translation="MKLLLSLFAQGWTHAQVLDNYPTLDNGTLQAVFAFSADCLHDEQ
                     YFAQALAT"
     gene            complement(394..549)
                     /locus_tag="PLB25_03705"
                     /pseudo
     CDS             complement(394..549)
                     /locus_tag="PLB25_03705"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(507..926)
                     /locus_tag="PLB25_03710"
     CDS             complement(507..926)
                     /locus_tag="PLB25_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOE40724.1"
                     /translation="MRVVFDTNVVLSALVFGGAMAGRLRKAWQSGDVIPLVNTDTAQE
                     LMRVLAYPKFHLSPQERTQLLADYLPYAQVIAMPETPPVVPECRDQYDLMFMRLARVG
                     QADALVSGDKDLLTLAEAFKSMSGCPILSVEAFFQQM"
     gene            complement(923..1141)
                     /locus_tag="PLB25_03715"
     CDS             complement(923..1141)
                     /locus_tag="PLB25_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006489017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOE40725.1"
                     /translation="MLAKMTSKNQLTLPKNIIKQVGATEYFDVEVKAGQIVLTPVRIQ
                     RADAVRAKLAELNLSEADITDAVAWARQ"
     gene            complement(1696..2634)
                     /locus_tag="PLB25_03720"
     CDS             complement(1696..2634)
                     /locus_tag="PLB25_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40726.1"
                     /translation="MQPTDPLPTPVTAPARSRLGQSAWWLLTLSYYVTVCLAHLQFSL
                     WLVRRRDGFFGRIAFADLVPGVAVVLGLLLVGWIALQLRHGARAWLTGGLWLLWLVLA
                     ALVDGFLTYSINEYVHYPQYALLAWLLARAMDPGKARWLVGRVLFWSTLMGIGDELLQ
                     YLWITTTYSDYLDFNDFLTNLLASAAGMLLYYGSAALPALPVKRPKPVISWLVAGLIS
                     AGLAIGLQSGRIVQTPTEKIPPGGIVATTDGRHVLYLQRGPDFYGSTQAGPRHGQYHV
                     LSPLPALLIMLGVGLVFAGYGRFRPGPGQAFGPFAH"
     gene            complement(2846..4873)
                     /locus_tag="PLB25_03725"
     CDS             complement(2846..4873)
                     /locus_tag="PLB25_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40727.1"
                     /translation="MRLFAVLLVALLGACALRAQTQYPAPLPQLFELAGVLAGEAAPD
                     ALQLAASPTLEQQLPLYKAMMAQPLNAPYRIGMLAQSFTEAQASPQQLVGLTGAVAGL
                     RLHRRPEATLTQQQARLRSAADPLASSLLWMADLASPGSTWSPALPDLVQLPDPLRFE
                     LAMMLASIGSANRFLQRALVKLPASATPALLRRQALDGVLEPFEEPDYRQLLPLIERE
                     ALLAGMLDLVAATARLKLFVSTAQQLPTVAWTLDTPMGQIVVDSSGQNNLHQLKDPLL
                     VLDVGGDDSYQFLQRSDSQRISVLLDHRGNDHYRSSAPGADPSSATMGYGILWDTEGN
                     DQYQGTQQAQGSALFGAALLVDGGGNNQFVANSHAQGHAIGGLAVLLGSPGHDTYVAQ
                     THAQGSAGPLGVALLLDPAGDDRYTLDNTPLHWPSSQLPSVNTSMEQGAGRGLGADFD
                     DGRYAAGGIGMLLDLAGNDHYVAQVFAQGAGYQEGLGVLVDDAGNDQFDAVWYAMGAA
                     AHQGAGILLKRGSGNDHYRASHVTSIGAAHDFSVAIFLDEGGDDHYALGDLGLGAAHD
                     NSFALFVDGAGDDHYQVTASACRALGVADSSGWGTRRESLLNLGLFMDLGGTDSYPPG
                     CPRASNNSIWPTPRRWPELKLRSEAGAGFDGVAPLPLAVRALTRPLHKPPQ"
     gene            complement(4907..5752)
                     /gene="asd"
                     /locus_tag="PLB25_03730"
     CDS             complement(4907..5752)
                     /gene="asd"
                     /locus_tag="PLB25_03730"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012349243.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HOE40728.1"
                     /translation="MSDRLAVLPQYLLPKQALTLLAGRIAGARGGRLTTRLIAWFVRR
                     YRVNMAEAANPDIASYASFNDFFTRELKASARPLAKAALICPVDGAISQFGPIVGQQI
                     FQAKGHHYSSTALLGGDAELASRFDDGHFATLYLSPRDYHRIHMPCDGVLRRMMYVPG
                     ELFSVNPTTARGVPGLFARNERVVCVFDGQHGPFVLVLVGATIVGSMATVWHGLVKAP
                     RSAEISQWRYDDGSKLLKQGQEMGRFLLGSTVVMLWPKGRMQFNPAWQAGGPVRLGEV
                     MANGD"
     gene            5899..6786
                     /locus_tag="PLB25_03735"
     CDS             5899..6786
                     /locus_tag="PLB25_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOE40729.1"
                     /translation="MTAARIPPIQCLLTFEALARLRSVTQTAEELCVTPSAVSHRVKQ
                     LEQIIGTKLFGRADFSLTVEGVEYLSHVRAGLAMLERFPGSALAPGKRKLRLAVTPTF
                     ARSLLIPRLRQFTEAYPEIDLTLQISIPLLDVVAEDADLMIRFGTGRYADVEHLCLIR
                     DAVTPLASSAFVREHGPFEVAQDLEGEPLLRSPLEPWRTWFDANGLDWPEPVDGSQFN
                     DIGLMCDGAAAGMGIALARVKLAQPWLDHGSLVRLGNNAVFAANVPSPHAHYLCWRTG
                     VMERWECLAFTEWLQKSLA"
     gene            complement(7137..7616)
                     /locus_tag="PLB25_03740"
     CDS             complement(7137..7616)
                     /locus_tag="PLB25_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HOE40730.1"
                     /translation="MSEANRPLLDRIDLKILRALQQDGRISNLKLAECVALSPTAVLA
                     RVQRLSKEGVIQGYEARLDAVKLGVGLMVFVEVLLDKTSAGIFEAFKAAVQVRPEILE
                     CHMVAGGFDYLLKTRLPDMNAYREFAGSVLWQLPGVRETRTYAVMEEVKSTTHLPLQ"
     gene            complement(7609..10041)
                     /locus_tag="PLB25_03745"
     CDS             complement(7609..10041)
                     /locus_tag="PLB25_03745"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE40731.1"
                     /translation="MPLLLRLLLPLALLVLAAGSVRAMLLWGDEVQAGALQSQRFVED
                     FQRLYGPRIAELQSLGQQASLQGVLDRGVAEYRSLVRLQWQSHSGDSLQAQAEPVALQ
                     VPAWVRFLQRVENMPNALQDSTRSFSLAYQGQSTGTLIVTLSAAPEINRIWESARLQM
                     WTMAGVLGVLCLLVPLVLLSSLRGLTKLSAAALLLPVQPELRIAPTGAREVQQLAVAF
                     NAMADDLQQARSQTQEQLLQTTWVANHDTLTGLPNRALLADRLHLALRRCERHQETLG
                     LCFIDLDHFKPINDQHGHEVGDQVLMTAARRMQAELRDTDTLARIGGDEFVMLLTSLQ
                     QPHEAQAIVERVLASLRLPIEIGDLVAHISGSAGLTLQGPVGSDAAAPEADLLLRQAD
                     QAMYQAKQAGRDRVRLWQSDPLGVLTDQQQWGERRTQAMQQGELRLLYQPKVHLISGE
                     VVGLEALIRWQHPDRGLLLPHEFLPQIEHSATIVAIGDWVMNAALQQMEAWARVGTGW
                     PVSVNVAPRQLMDQSFFSKLSLALLRCPHVKAHWLTLEILETSALGDLAQISAMVRDV
                     QSLGVHCSLDDFGTGYSSLSYLKELPVHEIKIDQTFVRNMLDDPGDLALVEGVISLGQ
                     VFGRHLVAEGMETPEHGVVLLRLGCEVAQGWCIARPMPVDDIAPWAEQYRPHSMWNQW
                     GKLKWDLNDIALLVAKYDHVKWVDHLIGAEDDAARQLSSDQLTHTGDCRFGLWYDGHG
                     RENYRRLSAFKAIAPVHRELHRLGHELIRLRDATRVEQAQAAAGRLLFCRSQMLQHID
                     HLHREVLAQHRV"
     gene            complement(10276..10893)
                     /locus_tag="PLB25_03750"
     CDS             complement(10276..10893)
                     /locus_tag="PLB25_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOE40732.1"
                     /translation="MPVVVVVNPKGGVGKSTLATQVAGYFAAQHQRVMLGDADRQQSC
                     KLWLSLRPDSARPIAGWTVDPDDIARPPKGTTHVVLDTPAGLRGKRLKEVLKLAHKVL
                     VPLQPSIFDICATRDFLDELTGSSHAAKTQIGLIGMRVDERTRTAEQLRQFVESVGLP
                     TLAYLRSTQNYIVLAGRGLTLFDLAPERVARDLAQWQPICQWLDQ"
     gene            complement(10927..11049)
                     /locus_tag="PLB25_03755"
     CDS             complement(10927..11049)
                     /locus_tag="PLB25_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40733.1"
                     /translation="MKKSPGLWFDAAAGQGTQGYDTFVTFLCLKTIDAGVADAP"
     gene            11068..12135
                     /locus_tag="PLB25_03760"
     CDS             11068..12135
                     /locus_tag="PLB25_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE40734.1"
                     /translation="MKIRYSLIAAGIVALSAAGTATAQEKTFRLLTWADYAPADIVAQ
                     FEKESGYKVEVTLSNNEEMISKLRATGGAGFDLAQPSQDRITGPQAEFGIYKPMDLSK
                     LKAELFIPSMLDATKKNTTLAGKVYGLPHIWGTDGLVVNTKLAKMDNYPDLCKADFKG
                     KTAVRLKRPTLLAFAFAAGKDPFALYGDAKAYTALMDETGKTITACKGNLKFFWDNKD
                     QLLNGMRSGEIVGAMMWDTGGWKLNNEKPEIQFIAPKAGALGWIDTFAIPAKGKNDAA
                     AYAWINFNMRPEIAAKVGASAGNFTASKGADQLADAKLKAQFAASFPEAALKNVKWYP
                     AVPAGIEDIEGKVLDRIKAAN"
BASE COUNT         2509 a         3815 c         3664 g         2272 t
ORIGIN      
        1 cttaacgcat caaacattaa agccgatctg ggcctggcca cggtatgccg cctggccaac
       61 gcgatgacat cagcgcattg aacgcgcgcc caatccattc gagcctggcc ccttcttgtc
      121 ccaattgacg caccatgccc atgggaatgt tttcattggc cagcagtttc atgtggccag
      181 ggcttgggca aagtactgtt cgtcgtgcag gcaatcggcg ctgaaggcaa acacggcctg
      241 caaggtgccg ttgtcaagcg tcgggtagtt gtccagcacc tgcgcgtgcg tccagccttg
      301 cgcaaacagc gacagcaata acttcaccga caggcgggtt ggctgtgagc caccgaatcg
      361 attcgagcct ggcccctttt tatacctggg tgcattttga gtcttctttt gaaaagcatg
      421 cagcacatat acagccgcgg gcaaccgtgc caggtacacc acacgaaacg tgccgtgcca
      481 acaccagacc gccgatcccc aaaacgctac atctgctgaa aaaaagcctc tacgctcaga
      541 atgggacatc ccgacattga cttgaatgcc tcagccaagg tcagcagatc tttgtcacca
      601 ctgaccaagg catcagcctg tcccacccgt gccaggcgca tgaacatcaa gtcatattga
      661 tcgcggcact cgggcacaac cggtggtgtt tcgggcatgg cgatgacctg cgcatagggc
      721 aggtagtcgg ccagcaattg tgttcgctcc tgcggcgaca ggtggaattt ggggtaggcc
      781 agcacgcgca tcagctcttg cgcagtgtcg gtgttgacca ggggaatgac atcgccgctc
      841 tgccaggctt tgcgcagccg cccggccatt gcgccaccaa acaccagcgc cgacaacacc
      901 acgttggtgt cgaacaccac cctcattggc gagcccaggc cacagcgtcc gtgatgtcgg
      961 cttcgctcaa gttcagctcg gccagcttgg cccgcacagc atcggcgcgc tgaatgcgca
     1021 ccggtgtcag gacgatctgc ccggccttca cttccacatc gaaatattca gtcgcgccga
     1081 cctgcttgat gatgtttttg ggcagggtca actgattttt tgaggtcatc ttggcaagca
     1141 tgggaatttt ttcagtaagg aatccttact ttagcactat tgcttgaagc gtgatggacg
     1201 actggcgcca tggacaggag ttcaatgccg cgacgagggc tggcgccaat gcggtatgcg
     1261 tcagtgcctt ggagaatctt ggagcaggag cagggggctt ggattcagac ggttcatggc
     1321 agttgggcaa aacagcaaaa gactggaagc gggctgacga tccacacggt gcaatcatgt
     1381 gcaaatggtc agggcagggc gcattccaat gtcacgcaaa tctacagttc caactacttc
     1441 gatagtcatg tctcggttag cgacggcggt aatttgcgct gcatggcagc agggttttgc
     1501 cgcatcaacc agggccttca gtctctaaca agtcaagtct ttgcacatca tgcgaagaat
     1561 tcaaacattc caagtcattc gtcgcgaggg cgcaactcct acaaattcaa ataggcgtcg
     1621 actgtttgtg taggagtcgc gccctcgcga cgaaggctca tcgattccgg tttgtccggc
     1681 ttaggttcag ggtcatcaat gcgcaaaagg tccaaatgcc tgacctgggc cgggccgaaa
     1741 ccggccataa ccggcaaaca ccagcccgac cccgagcatg atcagcaagg cgggcagcgg
     1801 tgacaacacg tgatactgac catggcgcgg tccggcttgc gtgctaccgt aaaagtccgg
     1861 gccgcgctgc aaatacagga catgacgacc atcagttgtt gccacaatgc cgccgggcgg
     1921 gattttttcg gtcggcgttt gcacgatgcg cccgctttgt agaccaattg ccaagcccgc
     1981 gctgatgagc cccgctacca gccacgagat caccggcttt gggcgcttca ccggcagcgc
     2041 tggcaaagcc gcactaccgt aatacagcag catgcccgcc gcagatgcca gcaggttggt
     2101 caaaaagtcg ttgaaatcca ggtaatcgct gtaggtggtg gtgatccaga ggtattgcag
     2161 caactcgtca ccgatgccca tcagggtgct ccagaacagc acgcggccca ccagccagcg
     2221 ggctttgcca gggtccatgg cgcgcgccag caaccaggcc agcaaggcgt attgtgggta
     2281 gtggacgtac tcgttgatgg aataggtcag aaaaccatcc accaaggcgg ccagcaccag
     2341 ccacagcagc cacaggcctc cggtcagcca ggctcgtgcg ccatggcgca attgcagtgc
     2401 aatccagcca accagcagca ggccaagcac cactgccacg ccgggcacca ggtcagcaaa
     2461 ggcgatgcgg ccaaaaaaac cgtcgcgccg acgcaccagc cacagcgaaa actgcaagtg
     2521 cgccaggcaa acggtaacgt aatagctcag tgtcagcagc caccaggcgc tttgcccaag
     2581 acgtgagcgg gctggcgctg tcactggtgt cggtagaggg tcggtcggct gcatcaggtg
     2641 actccgtatc aatgccattg ccagatcatt cggctccagc caccctggcg cagacatggc
     2701 aaacgagcgg caaggccaga tgacagcgac atggctgatt cagccatcga ttcacaatga
     2761 aatatatagc acgtcacgca ctgtccacgg gggctggagc cctattttgc ataaaaaagg
     2821 cttgtcgcaa gcagcatgct gttgttcatt gaggtggctt gtgcaaaggc cgggtcagcg
     2881 cccgaaccgc cagcggcagc ggtgcgacac cgtcgaaccc ggccccggct tcgctgcgca
     2941 gtttgagttc tggccacctg cgcggcgtcg gccagatgct gttgttgctg gcccgcgggc
     3001 accccggcgg gtaactgtcg gtgccgccca aatccatgaa cagccccaga ttgagcaggc
     3061 tttcacgccg tgtgccccag ccactgctgt cagcaacccc aagcgcgcgg caggcactgg
     3121 cggtaacctg gtaatggtca tcacccgcgc catcaacaaa cagggcaaag ctgttgtcat
     3181 gtgccgcgcc cagacccagg tcgcccagcg catagtggtc gtcaccgcct tcgtcgagaa
     3241 aaatggccac agaaaagtcg tgcgcggcgc cgatcgaggt gacatggctg gcccggtaat
     3301 ggtcgttgcc cgagccacgc ttgagcaaga tgcccgcgcc ctgatgcgct gccgccccca
     3361 tggcgtacca gactgcgtca aactggtcgt tgccagcgtc gtccaccagc acgcccaggc
     3421 cctcctgata gcccgcgccc tgcgcaaaca cttgcgccac gtaatggtcg ttgccagcca
     3481 ggtctagcag catgccaatg cccccggcgg catagcgtcc gtcgtcaaaa tcggccccca
     3541 gtccccgccc tgcgccttgc tccatcgagg tgttgacgct gggcaactgt gacgatggcc
     3601 agtgcagcgg cgtgttgtcc agcgtgtagc ggtcatcacc agccgggtcg agcaacagcg
     3661 ccaccccgag cggcccggcc gagccctgtg cgtgggtttg cgcgacatag gtgtcgtggc
     3721 ccggactgcc gagcaacacc gccagcccgc cgatggcgtg gccctgggca tggctgttgg
     3781 caacaaactg gttgttgccg ccgccatcga ccagtagcgc tgcgccaaac agggctgacc
     3841 cttgcgcctg ttgggtgccc tggtactggt cgttgccttc ggtgtcccac aagatgccat
     3901 agcccatcgt ggcactggac gggtcggcac ccggcgcgct gctgcggtag tgatcattgc
     3961 cccggtggtc aagcaagacg ctgatgcgct ggctgtcgct gcgctgcaag aattgatagc
     4021 tgtcgtcacc gccaacatca agcaccagca gcgggtcttt gagttgatgc aggttgttct
     4081 ggccgctgct gtcaaccaca atctggccca ttggcgtgtc aagcgtccag gccacggtgg
     4141 gcagttgctg cgcagtgctg acaaagagct tgagccgcgc ggttgcggcc accaggtcga
     4201 gcatgcccgc caacagggct tcgcgctcga tcagtggcaa caactggcgg tagtcgggct
     4261 cttcaaacgg ctccagcaca ccatccagtg cctggcggcg cagcaaggcc ggggtagcgc
     4321 tggcaggcag tttgaccaag gcgcgttgca aaaagcggtt ggctgaaccg atgctggcca
     4381 gcatcatggc caactcgaag cgcaacgggt cgggcaactg caccaggtcg ggcaaggccg
     4441 gtgaccaggt gctgccaggg ctggccaagt cagccatcca gagcagacta gaagccagcg
     4501 ggtcggcagc cgagcgcagt cttgcctgct gttgcgtcag ggtggcttcg ggccgacggt
     4561 gcagccgcaa acccgccacc gcgccggtta acccgaccag ttgctgcggc gatgcctgtg
     4621 cctcggtgaa ggactgagcc agcatgccaa tccggtaggg cgcattgagc ggctgcgcca
     4681 tcatcgcctt gtaaagcggc aactgctgct ccagcgtggg cgaagccgcc aactgcagcg
     4741 catccggcgc ggcctcgcct gccagcacac cggccaactc aaacagttgt ggcagcgggg
     4801 ccgggtattg cgtctgtgcc cgcagcgcgc acgcgcccag caaggccact agcagcacgg
     4861 cgaaaagtct caacccgctt ttttggcctg gcatcggctt gccacctcaa tcgccgttgg
     4921 ccatgacctc acccaaccgg accggcccgc cagcctgcca ggccgggttg aattgcatgc
     4981 gccctttggg ccacagcatc accaccgtgg aacccagcaa aaagcgcccc atctcctgcc
     5041 cctgcttcaa cagtttgctg ccatcgtcat agcgccattg gctgatttca gcgctgcgtg
     5101 gcgccttgac cagaccgtgc cagaccgtcg ccatgctgcc gacaatggtg gcccccacca
     5161 gcaccagcac aaacggtccg tgctggccat caaacacgca caccacccgt tcgttgcgcg
     5221 caaacaggcc cggcacgccc cgcgcggtgg tcgggttgac cgaaaacagc tcgcctggca
     5281 catacatcat gcggcgcagc acgccatcgc aaggcatgtg gatgcggtgg tagtcgcgcg
     5341 ggcttaagta cagggtggcg aaatggccat cgtcaaaccg gctcgccagt tcggcatcgc
     5401 ccccgagcag cgcggtgctg ctgtaatggt ggcctttggc ctgaaaaatc tgctgcccga
     5461 caatcggacc aaactggctg atggcgccgt ccaccgggca gatcagcgct gctttggcca
     5521 gcggtcgcgc actggccttg agttcgcggg tgaaaaagtc attgaagctg gcgtaactgg
     5581 caatgtccgg gttggcggcc tcggccatgt tgacccggta gcgtcgcaca aaccaggcga
     5641 tcagacgcgt tgtcaggcgg ccgccacgcg caccggcaat acgcccggca agcagcgtca
     5701 gggcctgttt tggcaatagg tactgcggca gcacggcaag gcgatcagac atgggtgaca
     5761 agcgggttgg gatgacaaaa agacagtagg gatgacggaa aacgcacttg ccaatggcca
     5821 ggcgcaggta gcaaggcagg gcatgcttgg ggattttacc cagcgccttg ggcggcaccg
     5881 aatgccacaa tgctggccat gaccgctgcc cgcataccgc caattcagtg cctgctgacg
     5941 ttcgaggcgc tggcgcgtct gcgcagcgtg acacaaaccg ctgaagaact ctgcgtgacc
     6001 ccaagcgcgg tcagccaccg ggtcaagcaa ctggagcaaa tcattggcac caagctgttt
     6061 ggccgggccg atttttcgct caccgttgaa ggtgtggaat acctgtccca tgtgcgcgcc
     6121 gggctggcca tgctggagcg ttttccgggt tcggcgctgg cccctggcaa acgcaagctg
     6181 cggctggcgg tgacaccgac ttttgcccgc tcactgctga ttccgcggct gcgccagttc
     6241 accgaagcct atcccgagat cgacctgacg ctgcaaatct cgatcccgct gctcgacgtg
     6301 gtggccgaag acgcagacct gatgatccgc tttggcactg gccgctatgc cgatgtggag
     6361 catctgtgcc tgatcaggga tgccgtcaca ccgctggctt cgtcggcgtt cgtgcgcgaa
     6421 catgggccgt ttgaggtggc gcaagacctc gagggcgagc cgctgctgcg cagcccgctg
     6481 gaaccctggc gcacctggtt tgatgccaat gggctggact ggcccgagcc agttgacggc
     6541 tcgcagttca acgacattgg cctgatgtgt gatggcgctg cggcgggcat gggtatcgca
     6601 ctggcacgcg tcaaattggc acagccctgg ctggaccacg gctcgctggt gcggctgggc
     6661 aacaatgcgg tgtttgctgc caacgtgccc agcccgcacg cccattacct gtgttggcgc
     6721 accggcgtga tggagcgctg ggaatgcctg gcgtttaccg agtggctgca aaagagcctg
     6781 gcttaacgtt cctttaatga tagcgcctgg cgcatacagg acaagggctg gagcctgttt
     6841 ttatactcaa ttcacaggta ttggccgagc aatgcaaagc ctgccttggc cgggccaaag
     6901 gcctgaagtt ggcacctgtt ggcacctctg ggcgcatgtt gtcctagcac acgaggcagg
     6961 cgcaacgacc ccaggcgcgt tgccagtggc ccgttcgggc gagtggcgtt gtgacccgat
     7021 gagtcaaccc catcgaaatt gaaagagcaa atgcgcatca gaacttgcag atgaaaacag
     7081 gcggtcaact ggggcatgga tgccttgcca gtctgccgct gtcagcctga aaccggtcat
     7141 tgcaacggca ggtgagtggt gcttttgacc tcttccatca ccgcgtaggt gcgggtttcg
     7201 cgcacaccgg gtaactgcca aagcacgctg ccggcgaact cccggtaggc gttcatgtcg
     7261 ggcagacggg tcttgagcag gtagtcaaag ccgcccgcca ccatgtggca ttccagtatc
     7321 tcggggcgta cctgcacggc agccttgaat gcctcaaaga tgccggcgct ggttttgtca
     7381 agcagtactt cgacaaacac catcagcccg acaccgagtt tgaccgcatc aagccgcgct
     7441 tcataaccct ggatcacccc ctctttgctc agtcgctgca cccgcgccag caccgctgtg
     7501 ggtgacagcg ccacgcactc ggccagcttc aggttgctga tgcgcccgtc ttgctgcagg
     7561 gcacgcaaga tcttcaggtc aatgcgatct agcaacggac ggttggcttc agacacggtg
     7621 ctgcgccagc acttcacggt gcaggtggtc aatgtgctgc aacatctgag accggcaaaa
     7681 cagcaaccgc ccggcagcgg cctgtgcctg ctcgacgcga gtggcatcgc gcaagcggat
     7741 caactcatgg cctaggcggt gcagctcacg gtgaaccggg gcaatcgcct tgaaagcgct
     7801 cagacgccgg taattttccc gaccatggcc gtcgtaccac agaccaaatc gacaatcgcc
     7861 agtatgggtc aattggtctg aagacagttg ccgcgctgcg tcatcttctg ccccgatcag
     7921 gtgatcaacc catttgacat ggtcatattt ggccaccagc agcgcaatgt cgttcaaatc
     7981 ccatttcagt ttgccccact ggttccacat tgaatgtggc cggtattgct cggcccacgg
     8041 cgcaatgtcg tcgaccggca ttggcctggc aatgcaccag ccctgcgcaa cttcgcagcc
     8101 cagccgaagc aacacgacac catgttcggg cgtttccatg ccctcggcca ccaaatgtcg
     8161 gccaaacacc tgtcccagtg aaatcacgcc ttccaccagg gccaaatcgc cggggtcatc
     8221 gagcatgttg cgcacgaaag tctggtcgat cttgatttcg tgcaccggca gttccttcag
     8281 atagctcagg cttgaatagc cggtgccaaa gtcatccagc gaacaatgca cgccaagcga
     8341 ctgcacatcg cgcaccatgg cgctgatttg ggccaagtca cccagcgcgc tggtttccag
     8401 aatttccagt gtcagccagt gggctttgac atggggacag cgcagcaagg ccaggctcaa
     8461 tttgctgaag aaactctggt ccatcagttg cctgggtgcc acattgacgc taaccggcca
     8521 gccggtgcca acccgcgccc aagcctccat ctgctgcaag gcggcgttca tgacccagtc
     8581 gccgatggca acaatggtcg cgctgtgctc gatctggggc agaaattcgt gcggcaggag
     8641 cagcccgcga tcagggtgtt gccagcgaat cagtgcctcc aggccgacca cttcaccgct
     8701 gatcaaatga accttgggct ggtacaacag tcgcagttcg ccctgctgca tcgcctgggt
     8761 ccgccgctcg ccccattgct gctggtctgt caggacaccc agcgggtcag actgccacag
     8821 gcggacgcgg tcacgcccgg cctgtttggc ctggtacatg gcctgatccg cctggcgcag
     8881 cagcaagtcg gcttcaggcg cagcagcgtc gctgcccacc gggccttgca aggtcagccc
     8941 ggcactgcca gagatgtgcg ccaccaggtc cccgatttca attggcaggc gcaacgaagc
     9001 cagcacccgc tccacaatgg cctgcgcctc gtgcggctgc tgcaggctgg tcagcagcat
     9061 gacaaactcg tcgccgccaa tgcgcgccag tgtgtcggtg tcacgcagct cggcctgcat
     9121 gcgtctggcg gcggtcatca gcacctggtc gcccacttca tggccgtgct ggtcattgat
     9181 gggtttgaaa tggtccagat cgatgaaaca cagccccaaa gtttcctgat gacgctcaca
     9241 acggcgcagt gccagatgca gacggtctgc cagcagggca cggttgggca agccggttag
     9301 cgtgtcatgg ttggccaccc aggtggtttg caacagttgc tcctgtgtct gcgagcgcgc
     9361 ctgttgcagg tcgtcggcca tggcgttgaa ggcaacggcg agctgctgca cctcgcgcgc
     9421 gccggttggc gctatgcgca gttcgggttg caccggcagc agcaatgcgg cggcgctgag
     9481 tttggtcaag ccgcgcaggc tgctgagcaa caccaaaggc accagcaagc acagtacccc
     9541 cagcacaccg gccattgtcc acatttgcag ccgggctgac tcccagatcc ggttgatttc
     9601 cggtgcagcc gaaagcgtca cgatcagggt gccggtgctc tgtccctggt acgccagtga
     9661 aaagctgcgg gtgctgtctt gcagggcatt gggcatgttc tcgacccgct gcagaaacct
     9721 gacccaggct ggcacctgca gcgcaaccgg ctccgcctgc gcctgcaagc tgtcaccaga
     9781 atggctttgc cactgcaagc gcaccagcga ccggtattcg gccacaccgc ggtccagcac
     9841 gccctgcaga ctggcttgct gtccaaggct ttgcaattcg gcaatgcgcg ggccgtaaag
     9901 acgctggaaa tcctcgacaa agcgctggct ttgcagcgcg cctgcctgaa cctcgtcacc
     9961 ccacagcagc atcgcacgca cgctgcccgc tgccagcacc agcaaggcca aaggcaacag
    10021 tagccggagc aacagcggca tggcaggggc gtgaaagcgg cgcatgtcgc gatattaagg
    10081 tatcactgtt gcgggttgcc gacggcccgg atgcgatggg tgcaatcagg gcacaccatt
    10141 gcgctggctg ttgtgcagca ccatcagcgc ttgcagaccg attgaagttt gtcaggcgcg
    10201 gtcaagcgcc gactggcaac ccaaagcggg tcagggactg aagcagccaa accgggaaac
    10261 tggccaaaac atggcttact gatccagcca ctggcaaatg ggctgccatt gggccaggtc
    10321 acgtgccacg cgttcaggtg ccaggtcaaa caaggtcagg ccacgcccgg caagcacaat
    10381 gtagttttgc gtgctacgca ggtaagccag ggtcggcagc ccgacagact cgacaaactg
    10441 gcgcaattgt tccgccgtgc gggtgcgctc atccacccgc atgccgatca ggccaatctg
    10501 ggttttggcg gcgtggctgc tgccagtcag ttcatcgaga aagtcgcgcg tggcacagat
    10561 gtcaaaaatg ctgggctgca acggcaccag taccttgtgc gccagtttca gcacctcttt
    10621 caggcgtttg ccgcgcaggc cggctggcgt gtcaagcacc acgtgggtgg tgcctttggg
    10681 gggccgggcg atgtcatcag ggtccaccgt ccagcctgca atcggtcggg cgctatcagg
    10741 gcgcagcgac agccagagct tgcaagactg ctggcggtcg gcatcgccca gcatgacgcg
    10801 ttggtgctgc gcggcaaagt aaccggcgac ctgggtcgcc agcgtggact tgccaacgcc
    10861 gcctttggga ttgacgacaa caacgactgg catggacagc tcctgatgca tgagaacagg
    10921 gctatcttag ggcgcatcgg ccacaccggc atcgatggtc ttgagacaca ggaatgtcac
    10981 aaaagtgtcg tatccttgcg ttccctgtcc ggctgccgca tcaaaccaga ggcccgggct
    11041 ttttttcatt cacccgagga ctcacccatg aaaatccgct actcattgat tgctgccggc
    11101 attgttgcgc tgagtgctgc tggcaccgca accgcacagg aaaaaacctt tcgcctgctg
    11161 acctgggccg actacgcccc ggccgacatc gtggcgcagt tcgagaaaga aagcggctac
    11221 aaggttgaag tgaccctgtc caacaacgaa gaaatgattt ccaagctgcg cgccactggc
    11281 ggtgccgggt ttgatctggc gcagccgtcg caagaccgca tcaccggccc gcaagctgaa
    11341 tttggcattt acaagccgat ggacctgagc aagctcaaag ccgagttgtt cattccatcg
    11401 atgctcgatg ccaccaagaa gaacacaacc ttggctggca aggtctacgg tctgccgcat
    11461 atctggggca ccgatggcct ggtggtcaac accaagctgg ccaagatgga caactaccca
    11521 gacctatgca aggctgattt caaaggcaaa accgccgtgc gcctgaaacg cccgacgctg
    11581 ctggcttttg cctttgccgc tggcaaagac ccgtttgccc tgtatggcga tgccaaagcc
    11641 tacaccgcgc tgatggacga aaccggcaag accatcaccg catgcaaagg caacctgaag
    11701 ttcttctggg acaacaagga ccagttgctc aacggcatgc gttcgggcga gatcgtcggt
    11761 gccatgatgt gggacacggg tggctggaaa ctgaacaacg agaagcctga aatccagttc
    11821 atcgcgccca aagcaggtgc attgggctgg atcgacacgt ttgccatccc cgccaagggc
    11881 aagaatgacg ctgcggccta cgcctggatc aacttcaaca tgcgccccga gatcgccgcc
    11941 aaggtgggtg cctctgctgg caacttcacg gcatccaaag gtgctgacca gttggccgat
    12001 gccaagctga aagcccaatt tgccgccagc ttcccggaag ctgcgttgaa aaacgtcaag
    12061 tggtatccgg ctgtgcccgc tggcattgaa gacatcgaag gcaaggtgct tgaccgcatc
    12121 aaggctgcaa actaagcccg ggccttggtt tccaccctgc ctgctgcgcc tgccaagctg
    12181 gtggcgcagc ctgacctgcg agccacccgc gtggccaagc actacggtgc tttccgcgcg
    12241 gtggatgatt tgtcgcttga
//
LOCUS       DAOSLL010000054        12227 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5224_length_12227_cov_4.148784, whole genome shotgun sequence.
ACCESSION   DAOSLL010000054 DAOSLL010000000
VERSION     DAOSLL010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 12227)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12227)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12227
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5224_length_12227_cov_4.148784"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            271..1401
                     /gene="apbC"
                     /locus_tag="PLB25_03765"
     CDS             271..1401
                     /gene="apbC"
                     /locus_tag="PLB25_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HOE40735.1"
                     /translation="MAAWLDSRFSEKNMSVTQQAILEALQAVVDPNTGRDFVSTKAIK
                     NLTVSGGDVAFEVELGYPAKSQIPGFRNDLMAAAKSVAGVVNVSVSVNTKITAHAVQR
                     GVALLPKVKNIVAVASGKGGVGKSTTAVNLALALAAEGASVGLLDADIYGPSIPMMMG
                     IEGKPESQDGQTMEPMENYGVQVMSIGFLVPQDEAMIWRGPMATQALEQLLRQTNWKD
                     LDYLIVDMPPGTGDIQLTLSQRVPMTGAVIVTTPQDIALLDAKKGIKMFEKVGVPILG
                     IVENMAVHVCSNCGHVEHIFGADGGKKMAAQYKMDYLGALPLAMQIRLQADSGHPSVV
                     SDPDSDVAGLYKAVARKVALSIAAKNKDFSSKFPSIKISRET"
     gene            complement(1649..2290)
                     /locus_tag="PLB25_03770"
     CDS             complement(1649..2290)
                     /locus_tag="PLB25_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HOE40736.1"
                     /translation="MCGLWGSAPVWATDDLAKVHAPVAAIQAPAESNKHMDAAADAPA
                     QALNADEPHGATQLFGSDLSQREIVLKPLEAIRFITSSAVASANSVASAAASVVSVAS
                     STVLDVAEVIQQGIASWYGAELHNRRTANGERFNMNDLTAAHRTLPFGTRLCAHSPKT
                     GKSVIVRINDRGPFAKDRIIDFSKAAAQALGIVHSRPDVVALLDSSDKRCAMG"
     gene            2632..2907
                     /locus_tag="PLB25_03775"
     CDS             2632..2907
                     /locus_tag="PLB25_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40737.1"
                     /translation="MKNEQLQSELDTLIATQAGLMAIVGSLMATHPHYERFQLHLTGL
                     LEVLLAGDAGKTFTASQRQQAREFVETLQHLNKAPAKIEPLASMPNL"
     gene            complement(2961..5792)
                     /locus_tag="PLB25_03780"
     CDS             complement(2961..5792)
                     /locus_tag="PLB25_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOE40738.1"
                     /translation="MAHFVVGIDLGTTHTVVACARLTTQRGGRAAFGKVTLFDVAQLV
                     SGGAVAALPLLPSVRYHAAADELVASELHLPWPPVGEVPAQPVVLGQFARTLGTQVPG
                     RLVTSAKSWLSHTLVDRLAPILPWGAPAEVAKVSPVAASASYLSHVRAAWNWQHPDAP
                     LEQQDLVLTVPASFDEGARALTLSAARLAGLDRLRLLEEPQAVFYDWLLRHRGKLAEQ
                     LTDTRLVLVCDVGGGTTDLSLIKVELIDGEPQLTRIGVGKHLMLGGDNMDLALAHLLE
                     ARLTPAADAPGAARLSASQLAQLMDRCRVAKEQLLASDAPAQVPITLLGGGARLIGGS
                     RSVMLTREDVEQTIVEGFFPQVAVSDEARQGRSGIVAFGLPYARDPAITRHIADFLRA
                     HAQTLPDTLLLNGGVFRADALVKRLEQTLAAWRKAPLRLLHNDNPDVAVARGAVAYAL
                     AQQGLAPKIGGGSARSYFLLLEHSDGICILPRGSEAGQEVLLSGQTFALRLGQPVRFH
                     LFSSVGDAPGHQPFKLGQRISLDTEGFLRLPPIATVLEAGSADGIRATGQNGPKDMAV
                     QLVTALTEVGTLEMHCVSLSDASQRWLLEFDLRQQTEAAAALPSGQQELGASRLADAI
                     KKIDRVFGTGSQPVLAKEVRQLRGQLEQVLGERERWATPELRQMFDALWQRARSRRRS
                     AEHERVWLNLAGYCLRPGFGYPLDDWRLQQLWTLFQPGVQHSHDKQVCTEWWTLWRRV
                     AGGLAAPEQLRLLDDFAANLHTNEIGSDESASKPVKGSDDDMLRLGASLERIPVDYKV
                     EIGEWLLRRLQKSQTASARRNGADVADDSRTLWALGRIGARQPFYGNSHDVVPPDTAT
                     AWVETLLALDWKRIEPAAFAATHLARVTDDRARDLPLTLRESVMARLTGMGAPAIWVE
                     MVRQRVELDAATEHRALGESLPPGLILMT"
     gene            complement(5803..7644)
                     /locus_tag="PLB25_03785"
     CDS             complement(5803..7644)
                     /locus_tag="PLB25_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007181849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HOE40739.1"
                     /translation="MSEAKFSIGIDLGTTHCALAFVDIAACDGEKTSHGILDIPQLTA
                     PGAVEASPLLPSFLYLPHADELAAGDLTLPWSDQQTDAVGEFARSRGALTPIRLVSSA
                     KSWLCHAGVDRRAAILPSEAPPEVARVSPLEATTRYLKHLRQAWDHAHPEAPFDQQDV
                     TVTIPASFDPAAREFTLEAAQAAGYGALTLLEEPQAALYNWIQASAGGWRKDVKPGEI
                     ILVIDVGGGTSDFSLIAVLEREGTLELHRVAVGDHILLGGDNMDLTLAHVVARKLAAE
                     GKQLDPWQMRALTYACRAAKENLLGDAALQAQPIVVPSRGSKLIGGSIKTELTQAEVQ
                     STILEGFFPEVAADSRPVSRTRSGLTQLGLPYAQDAGVTRHLAALLGRQVSATAELAG
                     FVGNTSADASFLHPTAVLFNGGVFKSNILAQRTLGTLNGWLAAEQAAAARMLAGADLD
                     LAVARGAAYYGYVRRGQGVRIRGGTARAYYVAIESSMPAIPGMEPPIQALCVAPFGME
                     EGTEADLQSQEFGLIVGAPVHLRFFGSSTRRQDHIGTLLDFWQPDELQELGEIHATLP
                     AEGRQPGETVQVTLRAVATETGTLALTAVATQSDEHWKVEFDVRSNG"
     gene            complement(7641..8225)
                     /locus_tag="PLB25_03790"
     CDS             complement(7641..8225)
                     /locus_tag="PLB25_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HOE40740.1"
                     /translation="MTQPNQPLNTAPQEPTFFRRVPIAFGAFFSILSNPKFAARVERL
                     QQGHEVAPNLATAPVAAAPAPVKAPNHDAALQLLGLLQRDARLIDFTQEDLSSYSDAD
                     IGGAARVVHEGCCKVLREHFSIAPVRAEAEGSRLTLEVGFDARAIRLTGNVLGQAPFK
                     GSLSHRGWRATEVRLPTLAASHDSSILAQAEVEL"
     gene            8717..9967
                     /locus_tag="PLB25_03795"
     CDS             8717..9967
                     /locus_tag="PLB25_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOE40741.1"
                     /translation="MTATLLLANILAAMLLQLLVLAGVAMWRRASSTPATGSPVPGDP
                     VAATRSLAWSGTRDFRVVRREYEDPAGSQCSFYLTPVDGAELPPFKPGQFLTFQLQPT
                     VADDVPQRQRPITRCYSLSDQPDPRHYRITIKRVPPPASQPGLPAGLSSNHCHDHVQV
                     GDVLQVKAPSGHFHLDVASLAPVVLVGGGIGITPMMSMLRWCQAEQPGRTVHLFYGLR
                     NSAEHAFKQTLEQLASTHSQVHLNVVYSQPGPDDVLGRDYQHTGHITVALLRQQLPLG
                     AYQFYVCGPAPMMEALVPELLAWGVPQQDVHFEAFGPASVRLPVASMPPAAILTSTPL
                     EVRFARSGRTLPWDGQQSNLLDFAERHGVAVDSGCRSGGCGSCETRLRKGTVSYASAP
                     DHDPAPGHCLLCVATPTSDLELEA"
     gene            9973..10551
                     /locus_tag="PLB25_03800"
     CDS             9973..10551
                     /locus_tag="PLB25_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40742.1"
                     /translation="MKARLIRREVLPFLLSFGLLVVAALLVDAALHLANIIWIGRYLG
                     IPGTLLILSSFGYSMRKRKLIKSGQPVQLLRWHERLAWLGSLLVLVHAGIHFNSILGW
                     LAVLAMLVNVASGLIGKYLLDSSRKRLEAARLEMRQSGLPAADLEDQTYWDSLTFDAV
                     KNWRTIHFPINLAFGVLGLGHIIAVFLFWGWK"
     gene            10548..11291
                     /locus_tag="PLB25_03805"
     CDS             10548..11291
                     /locus_tag="PLB25_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HOE40743.1"
                     /translation="MKRSWLMLVIAANLLVLAVLVFIYPHLMVSPGAVVVAHSEVATD
                     CFACHAPLRGASSGRCISCHALSDIGVRTSKGTPLPKKSVKTAFHQDLTEQNCMACHS
                     DHQGPKLTQRSRKPFSHALLKVAVRDQCLSCHKAPSDQLHRQVGGSCTQCHSQTAWKP
                     ATFEHDKYFLLDKDHSTECLTCHLKHDYRQYTCYGCHEHSPAKIQAEHQKEGIRDFEN
                     CVECHRSADGEPEKKGAGKGDGKGNKKED"
     gene            11305..11595
                     /locus_tag="PLB25_03810"
     CDS             11305..11595
                     /locus_tag="PLB25_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HOE40744.1"
                     /translation="MKCPTCVTTDLLMTERLNIEIDYCPQCRGVWLDRGELDKIIERS
                     SAEPVQMPAPAPQQMQRPPMPSYDVQHGYRDSHHENRHGYREKKSIWKELFD"
     gene            11669..>12227
                     /locus_tag="PLB25_03815"
     CDS             11669..>12227
                     /locus_tag="PLB25_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOE40745.1"
                     /translation="MISYLPVRFLTPKRLLWASISVALATITLKTLAWYVTDSVGLLS
                     DAMESFVNLASAIFALMMVTIAQRPADSDHPYGHHKAEYFSSGFEGILIVGVAIGIIW
                     AAAPRLFQPQPVQELGWGLGLSVLSSALNGLLAWVMFSSAKVHRSIALEADARHLVTD
                     VWTSVGVVVGLAAMAVTGWLWLDAVA"
BASE COUNT         2293 a         3724 c         3835 g         2375 t
ORIGIN      
        1 attccatgat gtggccgaca tggaacttgc cattggcgta gggcagggcg gtggtgacaa
       61 agagttggcg aggcaggggt gcggtggtca ttggcgatca atcaggtcac gggaggtcaa
      121 agtgggaatg cgctagcggc ttttgagtgc gctggtcaag ggtctgccaa ggcatcgggc
      181 ttgctttgat tctatcaatg gggggttcgg ccacccaggg tggcggccac gccatgggtc
      241 aacccgcatt tgcagtggcg gctcaggtct attgcggctt ggctagactc gcggttttcg
      301 gagaaaaata tgtctgtgac ccaacaagcc attctggagg ccctgcaagc tgtggtggac
      361 cccaataccg gacgcgactt cgtgtccacc aaggcgatca aaaacctgac agtgagcggc
      421 ggcgatgttg cctttgaggt ggaactgggc taccccgcca agagccagat tcccggcttt
      481 cgcaatgacc tgatggcggc agcaaaaagt gtcgcagggg ttgtcaatgt gtcggttagc
      541 gtcaacacca agatcaccgc gcatgccgtg cagcgcggcg tggccttgtt gcccaaggtc
      601 aagaacatcg tggcggtagc ttccggcaag ggtggtgtcg gcaagagcac caccgccgtc
      661 aacctggcgc tggcactggc tgccgaaggt gccagcgtgg gcttgctgga tgccgatatt
      721 tatggcccca gcatcccgat gatgatgggc atcgagggca agcctgaaag ccaggacggt
      781 cagaccatgg agccgatgga aaactacggt gtgcaggtga tgtcgatcgg ctttctggtg
      841 ccgcaggacg aggccatgat ctggcgcggc ccgatggcga cccaggcgct tgagcagttg
      901 ctgcgccaga ccaactggaa agacctggat tatctgattg tcgatatgcc gcccggcacc
      961 ggtgacatcc agctcacgct gagccagcgc gtgccgatga ccggtgcggt gatcgtcacg
     1021 acaccacagg acattgccct gctcgatgcc aaaaaaggca tcaagatgtt tgaaaaggta
     1081 ggcgtgccga ttttgggcat tgtggaaaac atggcagtgc atgtttgcag caactgtggc
     1141 catgtcgagc atatttttgg ggctgacggc ggcaagaaga tggccgccca atacaagatg
     1201 gactacctcg gtgccctgcc gctggcgatg cagatccgct tgcaggccga cagcggtcac
     1261 cccagcgtgg tgtcggaccc cgatagcgac gtggccggcc tgtacaaggc ggtagcgcgc
     1321 aaggtggcgc tgtcgattgc ggctaagaac aaggattttt caagcaaatt tccgtccatc
     1381 aagatatcca gggaaacctg attgggtcgc gctggacaaa ccgtttgtcg ctggccgtga
     1441 tcggaaaacc tggcgctgtt gcgggcgggt ctggcgcatc agcgcagtgc gggaactgag
     1501 cgggttgcaa aaatcaattg gcatctgcag cgactggcgc tagcccagat gccaacagcg
     1561 ccagcctctg gcagcacgcc caaccggtag ctgcgcctta gcccggactg cgaccggccc
     1621 cggcaaacgt gcccgattgg cagggcattc aacccattgc gcaacgcttg tccgacgaat
     1681 ccaacagggc gacgacatct ggccgggagt gaacaatgcc aagtgcctgt gctgctgctt
     1741 tgctgaaatc aatgatccgg tctttggcga acggaccacg gtcattgatg cggacgatga
     1801 cggacttgcc ggtcttgggc gaatgcgcgc ataacctggt gccaaacggc aatgtgcggt
     1861 gtgcagcggt caggtcgttc atattgaacc gctcgccatt ggcggtgcgg cggttatgca
     1921 actccgcacc gtaccaggat gctatgcctt gctggatgac ttctgcaacg tcgagcactg
     1981 tgctggaagc caccgagaca accgatgctg ccgccgatgc aaccgaattg gccgaagcca
     2041 cagcactgga agttataaac ctgatggctt ccagcggctt caagacgatt tccctttggc
     2101 tcaggtccga cccaaacaac tgcgttgcgc catgcggttc atcggcgttc aaggcctggg
     2161 caggcgcatc agccgcagcg tccatatgct tgttggactc agccggggct tggatggccg
     2221 ccaccggggc atggaccttg gcaaggtcgt cggtggccca gaccggtgcg ctgccccaaa
     2281 ggccgcacaa caataccagc aggtgatttc gaagccgccg cgcaggcgct aaataccgtg
     2341 tggcgcgcgc gcctggttga acggcagacg atgggtttgt gttttggatg tgcatgtaaa
     2401 aaggttcatt tggctttgcc ggtgaaaggg aagaggcaca aaaaaactga cgacaagcac
     2461 gcaaaatggg tgcgggtgaa agggcctcgg gcgcatttta gcggtttcgt cgatgctgac
     2521 cggcgcttgc cgcatgggca ccaggaaagc cgaccagatg atgtggggca tgcgctttgg
     2581 aaccttggca tcatctttgg tgtcgcacaa tatttgccca aggagatttc aatgaaaaac
     2641 gaacaactgc agtccgaact cgataccctg atagccaccc aggccggctt gatggcgatc
     2701 gtcgggtcac tcatggcgac acatccacat tacgaaaggt ttcagcttca cctgacgggc
     2761 ctgctggaag tgctgctggc cggtgatgcc ggtaaaacct ttaccgcaag ccagcgtcag
     2821 caggcgcgcg agtttgtcga aaccctgcaa catctcaaca aagcgcccgc aaaaatagag
     2881 ccactggcat ccatgccgaa cctgtagcgc cagatgccac agccgacggg tctgaacccg
     2941 gttagcacag ccaagccgct tcaggtcatc agtatcagcc caggcggcaa ggattcgcca
     3001 agcgcccgat gctcggtggc cgcgtccagt tcgactcgct ggcgcaccat ttcgacccag
     3061 atggctggcg cgcccatgcc ggtcagtcgc gccatcaccg actcgcgcag ggtcagcggc
     3121 aggtcgcgcg cacggtcgtc ggtgacgcgg gccaggtggg tagcggcaaa ggccgctggc
     3181 tcaatgcgct tccagtcaag tgccagcagc gtctcgaccc aggcagtggc ggtgtcgggc
     3241 ggcaccacat cgtggctgtt gccataaaac ggctggcgcg ctccgatgcg gcccagtgcc
     3301 cacagcgtcc ggctgtcgtc tgccacatca gcgccgttgc gcctggccga tgcggtttgg
     3361 gacttttgca gtcggcgcag cagccattcg ccaatttcga ccttgtagtc caccggaatg
     3421 cgctccagcg aggcacccag gcgcagcatg tcgtcatcgc tgcccttgac cggcttgctg
     3481 gcactttcgt cgctgccgat ttcgttggtg tgcaggttgg cggcaaaatc gtcgagcagg
     3541 cgcaattgtt caggtgcggc caggccgcct gcgacacgcc gccacaaggt ccaccattcg
     3601 gtgcaaacct gtttgtcatg gctgtgctgg acacccggct ggaacagcgt ccagagctgt
     3661 tgcaaacgcc aatcgtcgag cgggtagcca aatccggggc gcaggcaata tccggccaga
     3721 ttgagccaga cccgctcgtg ctcggccgag cggcgtctgc tgcgggcccg ctgccacagg
     3781 gcatcgaaca tctggcgtag ctcgggagtg gcccagcgct cgcgttcgcc cagtacctgc
     3841 tcaagttgcc ctcgcaactg cctgacttcc ttggccagca ccggctggct gccggtgcca
     3901 aacacgcggt cgattttttt gatggcatcg gccagcctgg acgcgcccag ctcctgctgg
     3961 ccagacggca gggcggctgc agcctctgtt tgctggcgca aatcaaattc cagcaaccag
     4021 cgttggctgg catcgctaag gctgacgcag tgcatctcca gcgtgcccac ctcggtcagc
     4081 gcggtgacca gttgtaccgc catgtctttt ggaccatttt ggcctgtagc ccttatgcca
     4141 tctgcgctac cagcttccaa tacagtagca atcgggggca gtcgaagaaa gccctcagtg
     4201 tccaggctga tcctttgccc gagcttgaag ggctggtgac cgggcgcatc gccaaccgag
     4261 ctgaacaggt gaaaccgcac cggctgcccg agccgaagcg caaaggtctg gccgctgagc
     4321 aacacctcct gaccggcttc gctgccgcgc ggcaggatgc agatgccgtc gctgtgttcc
     4381 agcaacagga agtagctgcg cgccgagcct ccgccaattt tcggggccag cccttgctgc
     4441 gccagcgcat aggccaccgc accgcgcgcc accgccacat ccgggttgtc gttgtgcagc
     4501 aggcgcagcg gggctttgcg ccatgctgcc agcgtttgtt caagccgttt gaccagcgca
     4561 tcagcacgaa acacgccgcc attgagcagc agcgtgtcgg gcagggtttg cgcatgggcg
     4621 cgcaaaaagt cggcaatgtg acgggtgatg gccgggtcgc gggcgtaggg caggccaaac
     4681 gccacaatgc cgctgcgccc ctgccgggcc tcgtcgctga cggccacctg cggaaaaaaa
     4741 ccttccacta tggtttgttc gacatcttcg cgcgtcagca tcaccgagcg gctgccgccg
     4801 atcaggcgtg cgccgccgcc tagcagcgtg atgggcacct gggccggtgc gtcgctggcc
     4861 agcaactgct ctttggcgac gcggcagcgg tccatcaact gggccaactg gctggctgac
     4921 aggcgcgccg cgccgggtgc gtcggcagcc ggggtcagtc gcgcctccag cagatgcgct
     4981 aacgccaggt ccatgttgtc gccgccgagc atcaggtgtt tgccgacccc gatgcgggtc
     5041 aactgcggct cgccgtcgat cagttcaacc ttgatcaggc tcaggtcggt ggtgccgcca
     5101 cccacatcgc acaccagcac caggcgggtg tctgtcaatt gctcggccag tttgccccgg
     5161 tgtcgcagca gccagtcgta aaacaccgcc tgcggctcct ccagcaagcg taaccggtcc
     5221 agcccggcca gccgtgcggc gctcagggtc agggcgcgcg ctccttcatc aaacgaggcc
     5281 ggcacagtca gcaccaggtc ttgctgctcc agcggggcat ccgggtgctg ccagttccag
     5341 gcggcgcgca catggctcaa gtagctggca ctggccgcta ccggcgacac cttggcgacc
     5401 tcggccggcg cgccccaggg cagaatcggt gccagccggt ccaccagggt gtgcgacagc
     5461 cagcttttgg cactggttac cagccggccc ggcacctgcg ttcccagcgt gcgggcgaat
     5521 tgccccagca ccaccggttg ggcaggcact tcaccgactg gtggccaggg caagtgcagc
     5581 tcgctggcca ccagttcgtc tgctgccgcg tggtagcgca ccgacggcag cagtggcagc
     5641 gcggccacag cgccgcccga caccaactgt gccacgtcaa acaatgtcac cttgccaaac
     5701 gcggcgcgac cgccacgctg tgtggtcagc cgggcgcagg cgacaacggt gtgggtggtg
     5761 ccaaggtcaa tgccgacgac aaagtgggcc atcagtgcga cgttagccgt tgctgcgcac
     5821 gtcgaactcg actttccagt gttcgtcgct ctgggttgcc acggcggtca gcgccagggt
     5881 gccggtttcg gtggcgacgg cgcgcagtgt cacctgcacc gtttcgcccg gttgtcggcc
     5941 ttcggcgggc aaggtggcgt ggatttcgcc gagctcctgc aactcgtcag gctgccagaa
     6001 atccagcagg gtgccgatgt gatcttgtcg ccgggtcgat gaaccaaaga agcgcaaatg
     6061 caccggcgca ccgacgatca ggccaaattc ctgcgactgc aaatctgctt cggtgccttc
     6121 ttccatgcca aacggggcca cgcaaagggc ttgtatcggt ggctccatgc cgggtatggc
     6181 tggcatcgac gattcgatcg ccacgtagta ggcgcgcgcc gtgccgcccc ggatgcgcac
     6241 gccctggccg cgccgcacat agccgtaata ggcggcaccg cgggccaccg ccaggtcaag
     6301 gtcggcaccg gccagcatgc gggcggcagc ggcttgctcg gctgccagcc agccattgag
     6361 cgtgcccaag gtgcgctgtg ccaggatgtt ggacttgaac acgccgccat tgaacaacac
     6421 cgccgtgggg tgcaaaaagc tggcatcggc actggtgtta ccgacaaaac cagccagttc
     6481 ggcagtggct gagacctgtc ggcccagcaa ggctgccagg tggcgggtga caccggcatc
     6541 ctgcgcgtaa ggcaggccga gctgcgtcaa accggaccgg gtgcggctga ccgggcgact
     6601 gtcagcggcc acctcgggga agaatccttc cagaatcgtg ctttgcacct cggcctgggt
     6661 cagttcggtc ttgatcgagc cgccaatcag cttggagccg cgactcggca ccacgatcgg
     6721 ttgcgcttgc agcgctgcat cacccagcag gttttccttg gcggcgcggc aggcgtaggt
     6781 cagcgcgcgc atctgccagg ggtcaagctg cttgccttcg gcggccagct tgcgcgccac
     6841 cacatgcgcc agcgtcaggt ccatgttgtc gccaccgagc aggatgtggt cgccaacggc
     6901 gacccggtgc agttccagcg tgccctcgcg ctcaagcacc gcaatcaacg aaaagtcgct
     6961 ggtgccgcca ccgacatcga tcaccaggat gatctcgccg ggcttgacat ccttgcgcca
     7021 gccccctgcg ctggcctgaa tccagttgta cagcgcggcc tgtggctctt cgagcaaggt
     7081 cagcgcgcca tagcccgcag cctgtgccgc ctccagggtg aattcgcgcg cggccgggtc
     7141 aaacgaggcc ggaatggtca ccgtcacatc ctgctggtca aacggggcct ccgggtgggc
     7201 atggtcccag gcctggcgca agtgcttcaa ataacgggtg gtggcttcaa gcggcgacac
     7261 ccgcgccact tcaggcgggg cctcgctggg caaaatggca gcgcgccggt ccaccccggc
     7321 atggcacagc cagcttttgg cactcgacac caggcgaatc ggggtcagcg cgccacgcga
     7381 acgggcaaac tcgccaaccg catcggtttg ctggtctgac caaggcaggg tcaggtcacc
     7441 ggcggccagt tcgtcggcgt gtggcaggta caggaacgac ggcagcaaag gcgatgcctc
     7501 gaccgcacca ggtgccgtca gttggggaat gtcgagaatg ccgtggctgg ttttttcgcc
     7561 gtcgcaggcg gcaatgtcca caaaggctag cgcgcaatgg gtggtgccaa ggtcaatgcc
     7621 aatggaaaat tttgcttcgc tcatagttcc acctcggctt gcgccagtat gctgctgtca
     7681 tggctggcgg ccagtgtcgg cagccgaacc tcggtggctc gccagccacg gtggctcaag
     7741 ctgcctttga aaggggcctg tcccagaacg ttgccggtca ggcggatggc gcgggcatca
     7801 aagccgactt ccagcgtcag gcggctgccc tcggcttctg cgcgcaccgg ggcgatgctg
     7861 aagtgctcgc gcagcacttt gcagcagcct tcgtgcacca cacgggcggc accgccaatg
     7921 tcggcatccg agtaactgct taaatcttcc tgagtaaagt cgatcagccg tgcatcgcgc
     7981 tgcaacaagc ccagcaactg cagggcagca tcgtggttgg gcgctttgac cggggccggt
     8041 gcagcggcaa caggtgcggt tgccagattg ggcgcgacct cgtggccctg ttgcaggcgt
     8101 tcaacgcggg cggcaaattt cgggttcgac aaaatactga aaaacgcacc gaaggcgatc
     8161 ggaacccgcc gaaaaaaagt cggttcctgc ggtgctgtgt tcaatggttg gtttggttga
     8221 gtcatggttt gatccgggtt gttctgggct gctttcggat ttgggtggtc gaaaagcaac
     8281 gaagcggcac cttgtggtgc cctgggtcag ttactgccgg gcagccatgc aaatcaggct
     8341 gcgtgcgcgg cgcgaatggc tattttttga cggcatcaat gctgtcgttc ttcggtattt
     8401 ccctggtgca aggcgaagca tcggaccgca cagcatgcgg tctagcggcc ggaattatag
     8461 ggatagcccg cccaggcatg ctgtgatcgt aacaatccct tgcgagtgcc gaccagcgct
     8521 ggctcagtag atggcaaaaa cctgtggttg ccgggggtgc ttgccgggat cgcaggcagt
     8581 acaaaggcgt tgggtacgga tcgcttttaa ggtagcccta agcaatcggg cgcatgcttg
     8641 gcagtcggtg ctctgtcgta ccgacccaat ggttttcaac agacatactg actccttcac
     8701 tccgggctgc tgattcgtga ctgcaacttt gcttctggcg aatattctcg cggccatgct
     8761 gctgcaattg cttgtgctgg caggtgttgc aatgtggcgc agagcatcct ccacaccggc
     8821 gaccggatcg cctgtgccgg gcgatccggt tgcggcaacg cgctcgctgg cttggtctgg
     8881 cacgcgcgat ttccgggtgg ttcgccgtga atatgaagac ccggcaggca gccaatgttc
     8941 gttttacctg acaccggtgg atggggctga gctgccacct ttcaagccgg ggcagttctt
     9001 gacctttcag ttgcagccaa ccgttgctga tgacgtgccg cagcgccagc gcccgatcac
     9061 ccgttgctat tctttgtcgg accagccaga cccaaggcat taccgcatca ccatcaagcg
     9121 tgtgccaccg cctgccagtc agcccgggct accagcaggg ctgtcgtcga accattgtca
     9181 tgaccatgtt caggtcggtg atgtgttgca ggtgaaagcc ccgtccggac atttccatct
     9241 tgatgtggcc agcctggccc cggtggtgct ggtcggtggc ggtattggca tcacaccgat
     9301 gatgagcatg ctgcgctggt gccaggccga gcagcccggg cgtaccgtgc atctttttta
     9361 cggtctacgc aacagcgcgg aacatgcctt caagcaaacc ctggagcagc ttgccagcac
     9421 ccactcccaa gtgcatttga atgttgttta cagtcaacct ggtccggatg acgtgttggg
     9481 gcgtgactac cagcacaccg gccatatcac ggtggccttg ctgcgccagc aattgccgct
     9541 tggggcctac cagttctatg tttgcggccc ggccccgatg atggaagccc tggttcccga
     9601 actgctggcc tggggcgtgc cgcagcagga tgttcatttt gaagcctttg gcccggcgtc
     9661 ggtaaggctg ccggtcgcct caatgccgcc tgccgcaatc ctgacatcaa cacctcttga
     9721 agtgcgcttt gcccgctctg ggcgcacttt gccgtgggac ggccagcagt ccaatctgct
     9781 ggactttgcc gagcgtcacg gtgtggcggt tgactcgggc tgccgctcag gtggctgtgg
     9841 cagttgcgaa acccgcctgc gcaaaggcac cgtgagctat gccagcgcgc cggatcacga
     9901 ccccgcgcct ggccactgcc tgctgtgcgt ggcgacaccc acttccgacc tggaacttga
     9961 ggcctgaccc cgatgaaagc ccgcctgatt cgccgtgaag tgttgccgtt tctgttgtct
    10021 tttggcctgc tggtggtggc ggccttgctg gtcgatgccg ccttgcacct ggccaacatc
    10081 atctggattg gccgctacct gggcattccc ggcacgctgc tgatcttgag ttcgtttggc
    10141 tattcaatgc gtaaacgcaa gctgatcaaa tcaggccagc cggtgcagtt gctgcgctgg
    10201 cacgagcgcc tggcctggct gggctccttg ctggtgctgg tgcacgccgg cattcacttc
    10261 aactcaattc tgggttggct ggcggtgctg gccatgctgg tgaatgtggc aagcgggctg
    10321 attggcaaat atttgctgga cagctcgcgc aaacgactcg aagcggcgcg actggaaatg
    10381 cgccaaagtg gcctgcctgc ggcggatctg gaagaccaga cctattggga cagcctgacc
    10441 tttgatgccg tcaaaaactg gcgcaccatt cactttccga tcaatctggc gtttggcgtt
    10501 cttggcctgg gccacatcat tgcggtgttt ttgttttggg gctggaaatg aagcgcagtt
    10561 ggctgatgct ggtgatcgcc gccaatctgc tggtgctggc agtgctggtg tttatctacc
    10621 cgcacctgat ggtcagcccc ggcgctgtgg tggtggcgca cagcgaggtc gccaccgact
    10681 gctttgcctg ccacgcgccg ctgcgtgggg cctcgtccgg gcgctgcatc agttgccacg
    10741 cgctgtccga cattggcgtg cgcaccagca aaggcacgcc gctaccaaag aagagcgtca
    10801 aaacagcgtt tcaccaggac ctgaccgagc aaaactgcat ggcctgccac agcgaccacc
    10861 agggcccgaa gctgacccag cgcagccgca aaccgttctc gcatgcgctg ctgaaggtgg
    10921 cagtgcgtga ccaatgccta agctgccaca aggcaccctc cgaccagttg caccgccagg
    10981 ttggcggtag ctgtacccag tgccacagcc agacggcctg gaagcccgcc accttcgagc
    11041 atgacaagta ctttttgctc gacaaggacc acagcaccga gtgtctcacc tgccatctga
    11101 aacacgatta ccggcaatac acctgttatg gctgccacga gcattcgcct gccaaaattc
    11161 aggccgagca ccagaaagag ggcatacgcg actttgagaa ctgtgtcgaa tgccatcgca
    11221 gcgccgacgg cgagccagag aaaaaaggcg caggcaaagg cgacggcaaa ggcaacaaga
    11281 aagaagactg aaccaaggaa ccctatgaaa tgccccacct gtgttaccac tgacttgctg
    11341 atgaccgagc gcctcaacat tgaaattgac tactgcccgc agtgtcgcgg cgtctggctg
    11401 gaccgcggcg aactcgacaa gatcatcgag cgcagttcgg ctgaacccgt ccaaatgcca
    11461 gcacctgcgc cgcagcaaat gcagcgtccg ccgatgccgt cctatgatgt ccagcatggc
    11521 taccgtgaca gccaccatga aaaccgccac ggctaccgtg aaaagaagtc catctggaaa
    11581 gagctgtttg actgatcgga tcaccccccc cggatgggca aatcgccatg aatgcaactt
    11641 cctctaaagt gttctgtcgc tcactatcat gatctcctac ctgccggttc gttttttgac
    11701 ccccaagcgc ttgttgtggg cctccatcag cgtcgcccta gcaactatca cactcaagac
    11761 actagcttgg tacgtgaccg attcggtcgg tttgctctct gatgcgatgg agtcgtttgt
    11821 caacctggcc agcgccattt ttgcgctgat gatggtgacc attgcgcagc gaccggctga
    11881 cagcgatcac ccttacggtc accacaaggc agagtatttt tcctcgggtt ttgagggcat
    11941 cctgattgtg ggtgtcgcca tcggcatcat ctgggcggcg gctccgaggc tgtttcagcc
    12001 gcagccggtg caggaactgg gctggggcct aggcctgtcg gtgctgagtt cggcactgaa
    12061 tggtttgctg gcctgggtca tgttcagttc ggccaaagtg catcgctcca ttgcgctgga
    12121 agccgatgcc cggcatcttg tcaccgatgt ctggacttca gtgggtgtgg tggtgggcct
    12181 ggctgccatg gccgtgaccg gctggctctg gcttgacgcg gtggccg
//
LOCUS       DAOSLL010000055        12176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5249_length_12176_cov_5.060309, whole genome shotgun sequence.
ACCESSION   DAOSLL010000055 DAOSLL010000000
VERSION     DAOSLL010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 12176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12176
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5249_length_12176_cov_5.060309"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..385)
                     /locus_tag="PLB25_03820"
     CDS             complement(<1..385)
                     /locus_tag="PLB25_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase N-terminal
                     domain-containing protein"
                     /protein_id="HOE40746.1"
                     /translation="MNIFHREQRSLFGEILDWMLTPLLLLWPVSLVLTWLVAQGIAGK
                     PFDRALEYNARAMAQLISIKRGRAEFALPRPARELLRADDSDTVYYQVLGARGEFLSG
                     ERDLPPPPADEKVTPDEVRIRDDVVK"
     gene            complement(382..1056)
                     /locus_tag="PLB25_03825"
     CDS             complement(382..1056)
                     /locus_tag="PLB25_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE40747.1"
                     /translation="MRILIVEDDQVLADGLLRSLRSSGAAVDHVANGTEADAALMTNT
                     EFDLLILDLGLPKMHGLEVLKKLRARGSSVPVLILTAADSVEERVKGLDLGADDYMAK
                     PFSLQELEARVRALSRRGMGAASSTIKHGPLIYDQGGRVATIDGVMVELSARELGLLE
                     VLLQRAGRLVSKDQLVERLCEWGEEVSNNAIEVYIHRLRKKIEKGPIRIATVRGLGYC
                     LEKIPG"
     gene            complement(1128..1613)
                     /locus_tag="PLB25_03830"
     CDS             complement(1128..1613)
                     /locus_tag="PLB25_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HOE40748.1"
                     /translation="MTETPSPSSPDSGWRQAHLGRWLALASQRFDARVLALMANNDQM
                     PLALSNLAARGHLTASHIHITRHLALSGSRLTELARLAGISKQGMGKLVDQCAAWGLV
                     QRQCDPHDARARLVVFTATGLAWLAAFEQAVKQAEAELRAELGLQVATVIAIGLEAYA
                     A"
     gene            1757..2839
                     /gene="recA"
                     /locus_tag="PLB25_03835"
     CDS             1757..2839
                     /gene="recA"
                     /locus_tag="PLB25_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HOE40749.1"
                     /translation="MDTTVKTLSPANAEKAKALQVALAQIEKQFGKGTIMRLGEGEVI
                     EDIQVVSTGSLGLDIALGVGGLPRGRVVEIYGPESSGKTTLTLQVIAEMQKTGGQCAF
                     IDAEHALDIQYAQNLGVNLQDLLISQPDTGEQALEIVDSLVRSGAVDLIVVDSVAALT
                     PKAELEGEMGDSLPGLQARLMSQALRKLTAHIKKTNCMVVFINQIRMKIGVMFGSPET
                     TTGGNALKFYASVRLDIRRTGTIKKGDEAIGNETKVKVVKNKVAPPFKTAEFDILFGQ
                     GISREGEIIDMGVNANILDKSGAWYAYNGEKIGQGRDNAREFLKENPSLSREIENKVR
                     VSLGIPLLAAAELPEAVAKVATKKAV"
     gene            2936..3430
                     /gene="recX"
                     /locus_tag="PLB25_03840"
     CDS             2936..3430
                     /gene="recX"
                     /locus_tag="PLB25_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HOE40750.1"
                     /translation="MAFDQPSLKSRALRLLATREYSRAELQRKLAPFEDAVGTLAAAL
                     DELQAKGFIDEQRVLASVLNRSAAKLGAARIQQELQKKGLAPQAVADALAALRATELE
                     RAQAVWRKKFHGAPADASAYAKQARFLAARGFSSELVSRLLSQAGHPPSAQSDTDESE
                     PNWL"
     gene            complement(3644..4579)
                     /gene="argC"
                     /locus_tag="PLB25_03845"
     CDS             complement(3644..4579)
                     /gene="argC"
                     /locus_tag="PLB25_03845"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HOE40751.1"
                     /translation="MQKIFIDGEAGTTGLQIRERLQTMPGIELLSIAPEHRKDASAKR
                     ALMSQADLVVLCLHDEAAIESVAVIEDLERATGRNGPKIIDASTAHRTAPGWVYGFPE
                     LVPGQREAIQAARRVANPGCYATGAIALIRPLVDAGLMPRDFALCLPSVSGYSGGGRP
                     MIEAYEQAMAPAFELYGLGLKHKHIPEILAYTGLTRRPLFVPSVGNFRQGMLVQLPLH
                     LELLPGQPSIADLHAVLARHYAGSEWVSVPPATPDNKLDAVQLADSNFMELRVFGNRD
                     TDDNFRHALLVARLDNLGKGASGAAVQNLKLMLGM"
     gene            4740..5909
                     /gene="sucC"
                     /locus_tag="PLB25_03850"
     CDS             4740..5909
                     /gene="sucC"
                     /locus_tag="PLB25_03850"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HOE40752.1"
                     /translation="MKIHEYQGKEILRQFGVPVPRGIAAFTVQEAVEAAQKLGGPVWV
                     VKAQIHAGGRGKGGGVKLARSIDDVKKLAGEILGMQLVTHQTGPMGQKVRRLLIEDGA
                     DIKKEYYVSAVTDRASQRVAMIVSSEGGMSIEEVAHDTPEKIITELVDPAVGLTLAQA
                     TKLSNAIGVPAGSTAQAVDVLQKVYKVYMDTDASLVEINPMILEGNGNIKAIDAKFNF
                     DANALYRHPEIVAYRDLDEEDAAEVEASKFDLAYISLDGNIGCLVNGAGLAMATMDTI
                     KLFGAEPANFLDVGGGATPEKVTEAFKIMLKNDKVKAILVNIFGGIMKCDTIAAGVIT
                     ACKAVNLSVPLVVRMKGTNEDIGKKMLAESGLPIISADTMADAAEKVVAAVKAAV"
     gene            5928..6821
                     /gene="sucD"
                     /locus_tag="PLB25_03855"
     CDS             5928..6821
                     /gene="sucD"
                     /locus_tag="PLB25_03855"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HOE40753.1"
                     /translation="MSILINKDSKVITQGITGKTGQFHTEKCQEYANGKNCFVAGVNP
                     KKAGESILGIPIFASVKEAAAQTGATVSVIYVPPPGAAAAIWEAVEADLDLAICITEG
                     IPVRDMLEVRNKMRAKEAAGGKKTWLLGPNCPGLITPEEIKIGIMPGHIHRKGRIGVV
                     SRSGTLTYEAVAQLTEIGLGQSSAVGIGGDPINGIKHIDIMRAFNEDPDTDAVIMIGE
                     IGGPDEAEAARWCKLNMKKPIVGFIAGVTAPAGKRMGHAGALISGGADTAEAKLAVME
                     ECGFKVTRNPSEMAKLLKALL"
     gene            7192..7980
                     /locus_tag="PLB25_03860"
     CDS             7192..7980
                     /locus_tag="PLB25_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HOE40754.1"
                     /translation="MNYTLFAQTDPGLVRSNNEDSVTFDETFHVAVLADGMGGYNAGE
                     VASGMATTFIKSELVRWFSKGPQQVDAALVSRAIEICVKKSNFSILNAAQTNPNYAGM
                     GTTLVVGVFLASTLVLAHVGDSRCYLWRDGELSQVTKDHSMLQEQVDAGVLTAEQAAV
                     APGKNLLTRALGVEETVRVEIHNYAVHDGDLYLMCSDGLSDMLDSRAISAILAGAEPL
                     STMASQLVNQAKDSGGRDNITVLLAQASDGPTKPGLMSRFFGAQ"
     gene            8039..8680
                     /locus_tag="PLB25_03865"
     CDS             8039..8680
                     /locus_tag="PLB25_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HOE40755.1"
                     /translation="MPKVSVVFDGLVIKEVELTKQRTTLGRRPYNDIVIENLTVSGEH
                     LALNMQSDQVTAEDLNSTNGTYIDGNAIKLHALRDGDMLEVGKYKIRFDSGQDRDNFE
                     KTMIFKPSAAVQPIPPVPAPAPTPELHGWIKVLSGAAAGREMALTKVVTTVGKPGISV
                     AAITQRRHGFFVHHVEGNDRLLLNGLPVGEDPSPLRHGDQIALTGTQMQFLQR"
     gene            8734..11105
                     /locus_tag="PLB25_03870"
                     /pseudo
     CDS             8734..11105
                     /locus_tag="PLB25_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
     gene            complement(11243..>12176)
                     /locus_tag="PLB25_03875"
     CDS             complement(11243..>12176)
                     /locus_tag="PLB25_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOE40756.1"
                     /translation="LDLHAAGSFECVSKHMLGSRLTHGIFMTGGFFLGPRDFYERLRT
                     MAPQDLAKIDMTRIDFINQLYGNDALKRAQRRNARFMNTTMVVTLLGAAASDALESGQ
                     VVSGVGGQYNFVAMSHALADARLIMMLRATHDNKDGLKSSIVWNYGHVTIPRHLRDVV
                     ITEYGIAELRGRSDGEVVKRLIAIADSRFQGELVKQAQAHGKLEADYEVPECYRHNLP
                     EVLAAKLKPWAEAGLLPDFPFGTDLTDDELHMVRAMKKMKHASQHPSELLTMAVKSLW
                     QGKEAPPAYLERLGLADVHSFKDRVMRRLFAGNL"
BASE COUNT         2775 a         3408 c         3449 g         2544 t
ORIGIN      
        1 ccttgaccac gtcatcgcgt atgcgcacct cgtctggagt caccttttcg tcggccggag
       61 gtggcggcag gtcgcgctcg ccactcaaaa attcgccgcg cgcccccagt acctggtaat
      121 agacggtatc ggaatcgtcg gcgcgcaaca gttcgcgcgc cggtcgcggc aaggcaaact
      181 cggcccgtcc gcgcttgatg ctgatgagtt gcgccatggc ccgggcgttg tactcaagcg
      241 cccggtcaaa aggtttgccg gcaataccct gtgccaccag ccaggtgagc accagactga
      301 ccggccacag cagcagcagc ggtgtcagca tccagtccag gatttcgcca aacagcgagc
      361 gctgttcgcg atgaaatatg ttcacccggg tattttttcc aggcagtagc ccagacctcg
      421 cacggtggca atgcggatcg ggcctttttc gattttcttg cgcaaccggt ggatatacac
      481 ctcaatggcg ttgttgctga cctcttcgcc ccattcgcac agccgctcca ccagttgatc
      541 cttgctgacc agacggcctg cgcgctgcaa cagcacttcg agcagaccga gttcgcgcgc
      601 tgacaactcc accatgaccc catcgatggt tgccacgcgg cctccctggt cataaatcag
      661 cggaccgtgc ttgatggtgc tgctggcggc acccatgcca cgacgactca gggcgcgcac
      721 ccgcgcttcg agttcttgca gactgaaggg tttggccatg tagtcatcag caccaaggtc
      781 caggcctttg acacgttctt cgacgctgtc ggcggcggtc agaatcagca ctggcaccga
      841 cgagccacgc gcccgcaatt ttttcaacac ctccagacca tgcatttttg gcagaccaag
      901 gtccagaatc agcaaatcaa actcggtgtt ggtcatcaat gcggcatcgg cctcggttcc
      961 gttggccaca tggtcgaccg ctgcgcctga actgcgcaag ctccgcagca aaccgtcggc
     1021 cagtacctgg tcgtcctcga ctatcaaaat tcgcatggct gtttcctaaa tggggcggct
     1081 tggccctggg gttatggcgg tgggccgtga atgtgatcaa aaccatttta tgcggcgtaa
     1141 gcttcaagtc cgattgcgat gacggtggcc acttgcaagc caagttcggc acgcaattct
     1201 gcttctgcct gtttgacggc ttgctcgaac gctgccagcc aggccagccc ggtggcggtg
     1261 aacaccacca ggcgcgcccg tgcgtcgtgc ggatcacatt ggcgctgcac caagccccag
     1321 gcagcgcact gatcgacaag tttgcccatg ccttgtttgc tgatgccagc cagtctggcc
     1381 agctcggtca ggcgcgagcc gcttagcgcc agatgccggg tgatgtgaat gtgcgaggcg
     1441 gtcaggtgac cccgcgcggc cagattgctc agtgccagcg gcatctggtc gttattggcc
     1501 atcaaagcca gcactcgcgc atcaaaacgc tgcgatgcca aggccagcca gcggcccaga
     1561 tgggcttgcc gccaaccgct atcgggcgag ctaggcgacg gggtttcagt catgaccgca
     1621 tagtaaacca aattgaccaa aaaaagctct tttagatttc aagtttgaac taaactactg
     1681 tttaagcatc cagcctgtcg ttacaaccag acgatgccaa tcacctcaaa cttgttgaac
     1741 cctcaggaga aatgccatgg acacaaccgt caaaaccctc agccctgcga atgccgaaaa
     1801 agccaaagcg ctgcaagttg cgctggcgca gattgaaaag cagtttggca aaggcaccat
     1861 catgcgcctg ggtgaaggcg aagtgattga agacatccag gtggtgtcca ccggctcgct
     1921 gggcctggac attgcgctgg gcgttggtgg cctgccgcgc gggcgagtgg tggagattta
     1981 cggcccggag tcgtccggca agaccaccct gacattgcag gtgattgccg agatgcaaaa
     2041 aaccggcggt caatgtgcct tcatcgatgc cgagcacgca ctcgacatcc agtatgcgca
     2101 aaacctcggt gtcaacctgc aagacctgct gatcagccag cccgacaccg gcgaacaggc
     2161 gcttgagatt gtcgatagcc tggtgcgctc cggtgcggtc gatctgatcg tcgtcgattc
     2221 ggtggcggcc ctgaccccca aggccgaact tgaaggcgaa atgggcgaca gcctgccagg
     2281 cctgcaagcc cgcctgatga gccaggcgct gcgcaagctg actgcccaca tcaagaaaac
     2341 caactgcatg gtggtcttca tcaaccagat ccgcatgaag atcggggtga tgtttggttc
     2401 gcccgagacc accactggcg gcaatgcgct gaagttctac gcctcggtgc ggctcgacat
     2461 ccgtcgcacc ggcaccatca agaaaggtga tgaagcgatt ggcaacgaaa ccaaggtcaa
     2521 ggtggtcaaa aacaaggtgg cacccccttt caaaaccgct gaatttgaca ttctgtttgg
     2581 ccagggcatc agccgcgaag gcgaaatcat cgacatgggt gtcaacgcca acatcctcga
     2641 caagtcgggt gcctggtacg cctacaacgg tgaaaaaatt ggccagggcc gtgacaacgc
     2701 ccgtgagttc ctgaaagaaa acccaagcct ttcgcgcgag attgaaaaca aggtccgggt
     2761 gtcactcggc attccgctgc tggccgccgc cgagctgccc gaggcggtcg ccaaagttgc
     2821 aaccaaaaaa gccgtctagc ccatagctgg cttgcgctgg aagctacttt aataatagct
     2881 attagagacc tcagggcggt cttgccaaag cacaccaaca gcgggttgta accacatggc
     2941 ctttgaccaa ccatcgctca aaagccgtgc gttgcggctg ctggctaccc gcgagtattc
     3001 gcgcgccgag ctgcaacgca agctggcgcc atttgaagac gcggttggca ctctggctgc
     3061 cgcacttgat gaactccagg ccaaggggtt tatcgacgaa caacgggtac tggcgtcggt
     3121 gctgaaccgc agcgcagcca aacttggcgc agcccgcatc cagcaggaac tgcagaaaaa
     3181 ggggctggca ccgcaggccg tagccgatgc cctggcagcg ttgcgtgcca ctgaacttga
     3241 gcgtgcgcag gcggtgtggc gcaagaagtt tcatggcgca cctgcagatg ccagtgccta
     3301 tgccaaacag gcgcgctttc tggcggcacg gggcttcagt agcgagctgg tgagccgact
     3361 gctgtcgcag gcgggccacc caccgtcagc tcagtcagac accgatgaat ctgagccaaa
     3421 ttggctttga gcccttatgc agcatgcgct gccagcacct ttattgatag cgttacaagg
     3481 aaccagtcgg accgatgaaa gcaaaccaaa gatcggctcc agcgagggca gtttgtgcca
     3541 gcttctgact cacggccagg ccatgggtca agaggtcggc gcaaactggt tttttttgcg
     3601 gtcagtgcgc agccgatgcc cgatcagtcc gaccggcttt tgctcacatt ccaagcatta
     3661 gcttgaggtt ttgcacggcc gccccgctcg cacctttgcc caggttgtcc aaacgcgcca
     3721 ccagcaaggc gtgcctgaag ttgtcatcag tatcccggtt gccaaacacg cgcagttcca
     3781 tgaagttgct gtctgcgagt tgcaccgcat caagcttgtt atctggcgtg gctggcggca
     3841 cgctgaccca ttcgctgcca gcatagtgtc tggccaatac cgcgtgcaaa tcggcaatgc
     3901 tgggctgccc aggcagcagt tcaaggtgca acggcagttg caccagcatg ccttgcctga
     3961 agttgcccac cgaaggcacg aacagggggc gacgcgtcag gccggtgtaa gccagaattt
     4021 ccggaatgtg tttgtgcttg aggcccagcc cataaagctc gaaggctggt gccatggcct
     4081 gctcataggc ttcgatcatg ggtcggccac cgcccgagta gccactgacc gaaggcaggc
     4141 acaaggcaaa gtcgcgcggc atcagcccgg catcgaccaa ggggcgaatc agcgcgattg
     4201 cgccagtcgc gtagcaaccc ggattggcga cccggcgcgc cgcctgaatc gcctcgcgct
     4261 gccctggcac cagttctgga aagccataga cccagcctgg agcggtacgg tgagctgtcg
     4321 atgcgtcaat aattttgggg ccatttcggc ctgtagccct ttccaggtct tcgatgacag
     4381 ctactgattc aatagctgct tcatcatgca ggcacaacac caccaggtca gcctgactca
     4441 tcagtgcgcg tttggcgctg gcatccttgc gatgctcagg cgcaatgctg agcagctcga
     4501 tgccaggcat tgtctgaagc cgctcacgga tttgcaaacc ggtggtacct gcttcgccgt
     4561 cgatgaatat tttttgcatg ttggattgaa actcggaaaa gtgacgttgc tgtaagtctg
     4621 ataaaagatt gacgcagcgc aacatgatac atttccaagc tgcatcctta gtggcgttga
     4681 ccgatctttt ccagaccagt caaatggcca tccaaccaac cattcctgag agagacctca
     4741 tgaagattca cgagtaccaa ggcaaggaaa tcttgcgtca atttggtgtg ccagtgcctc
     4801 gcggcattgc tgcattcacc gttcaagagg cggtcgaagc cgcccaaaaa ctcggcggcc
     4861 cggtttgggt tgtcaaagcg cagattcatg caggtgggcg tggcaagggg ggcggagtca
     4921 agttggcacg ctctattgac gatgtcaaga aacttgctgg tgagattctg ggcatgcaac
     4981 tggtcacgca tcagaccggc ccgatggggc aaaaagtccg ccgactcctg attgaagacg
     5041 gtgccgacat caaaaaagaa tattacgtat ccgcggtcac tgatcgcgcc agccagcgcg
     5101 tggcgatgat cgtctcctct gaaggcggca tgtcaattga agaggtggca cacgacaccc
     5161 ccgagaaaat catcacagag ctggttgatc cagccgtcgg gctgactctg gcccaggcca
     5221 ccaaactgtc caatgccatc ggcgtgccag ccggttctac cgcccaggct gtggacgtgc
     5281 tgcaaaaggt ttacaaggtt tacatggaca ccgatgcctc gctggtcgag atcaatccga
     5341 tgatcctcga aggcaacggc aacatcaagg caattgatgc caagttcaac tttgatgcca
     5401 atgcgctgta ccgtcatccc gaaatcgtgg cttaccgcga tctcgacgaa gaagacgcag
     5461 ccgaggttga agccagcaag tttgacctgg cctacatcag cctggacggc aacattggtt
     5521 gcctggtcaa cggtgccggt ctggccatgg ccaccatgga caccatcaag ctgtttggcg
     5581 cagagccggc caacttcctt gatgtaggcg gcggtgccac cccggaaaaa gtcaccgaag
     5641 ccttcaagat catgttgaaa aacgacaagg tcaaggcaat tctggtcaac atctttggcg
     5701 gcatcatgaa gtgcgacacg attgccgccg gtgtcatcac cgcctgcaag gcagtcaacc
     5761 tgtctgtgcc gctggtggtg cgcatgaagg gcaccaatga agatattggc aaaaagatgc
     5821 ttgcagaatc cggcttgccg atcatcagtg ccgacaccat ggccgatgcg gctgaaaaag
     5881 tggttgctgc ggtcaaggcc gccgtttgag actgaatagg aaaaatcatg tccatcctca
     5941 tcaataaaga ctccaaggtc atcacccagg gcattaccgg caagaccggt cagttccaca
     6001 ctgaaaaatg ccaggaatac gccaacggca agaactgctt cgtagccggc gtgaacccca
     6061 aaaaagcggg tgaatccatt ttgggtattc caatttttgc gtcagtcaaa gaggccgcag
     6121 cacagaccgg tgctaccgtg tcagtgattt acgtgccgcc acccggtgcc gcggctgcca
     6181 tctgggaagc agttgaagcc gacctggatc tggcgatctg catcaccgaa ggcattccgg
     6241 tgcgagacat gctggaagtg cgcaacaaga tgcgcgctaa agaggctgcg ggcggtaaaa
     6301 agacttggct gctcggcccc aactgcccgg gtttgatcac tcctgaagag atcaagatcg
     6361 gcatcatgcc tggccatatt caccgcaagg gtcgcattgg tgtggttagc cgctcgggca
     6421 cgctgaccta tgaagcagtt gcccagttga ccgaaatcgg cctcggccag tccagtgccg
     6481 ttggcattgg cggcgacccg atcaatggca tcaagcacat cgacatcatg cgcgccttca
     6541 atgaagatcc ggataccgat gcggtcatca tgatcggtga aattggtggc cctgatgaag
     6601 cggaagcagc tcgctggtgc aagctcaaca tgaaaaagcc aattgttggt ttcatcgcag
     6661 gtgtgactgc gcctgcgggc aagcgcatgg gccacgcagg tgccttgatt tcaggtggag
     6721 ccgacacggc cgaagctaag ctggctgtca tggaggaatg tggttttaag gttacgcgca
     6781 acccgtctga aatggccaag ctgctcaagg ctctgttgta aacagcagcc gcaggcaaac
     6841 cgccaagcct gaccccatgg caggcagctc aaagccggat ttcctgatgg actatccggt
     6901 ttttttgcgt tcaggctccc ccaaaggccg gtaacccatg gaaaagctct tccgggcatt
     6961 tacttgttac cgaactttga catcccggct tggcggtgtc cggcgggcat tggcgttgaa
     7021 ggcctgattt tccctgcgcc cgtgcgctcg aaatgtcgcg tcactcgcat tcataatcag
     7081 aatattccga gacgtttttg aaatggctgg catatcatcg ttacttctga gaccaggtat
     7141 ggtgagaccc gaagcaaagg cacttgattc tgaaattgaa tgatttcact tatgaactac
     7201 acactctttg cgcaaaccga tccaggtttg gtccgctcaa acaacgaaga ctcagtcacc
     7261 tttgacgaga cctttcatgt ggctgtgctg gcggacggca tgggtggtta caacgcgggt
     7321 gaggttgcga gcggcatggc gacgactttc atcaaatcgg aactggtacg gtggttttcc
     7381 aaaggcccac agcaagttga tgctgcgctg gtttcccggg ctatcgaaat ttgcgtcaag
     7441 aagagtaatt tttccatcct gaatgccgct caaaccaacc ccaactatgc tggaatgggc
     7501 accacactgg tagttggggt gtttttggca tccacgctgg tgctggcgca tgtgggagat
     7561 tcgcgctgct atctttggcg agatggtgag ctgtcgcagg taacaaaaga ccattccatg
     7621 ctgcaagagc aagtcgatgc aggggtgttg actgctgaac aggcggcagt ggctcccgga
     7681 aaaaatctgc tgactcgtgc gctaggggtt gaagagaccg tcagggtcga gattcataat
     7741 tacgcggttc atgatggcga cctctacctg atgtgctcgg atggactaag cgatatgctc
     7801 gattcacgtg ctatttctgc cattctggct ggtgctgagc cgctttcgac aatggcctca
     7861 caactggtga atcaagccaa ggacagcggc ggccgggaca atatcacggt tttgctggca
     7921 caagcatcgg atggccccac caaaccaggc ttgatgtcgc gtttttttgg tgcgcaatga
     7981 cacagcggct tggcttgatc gttgtcaaac ccatatactt gcacaacagg aggtgaccat
     8041 gcctaaggta tcggtagtat ttgacggtct cgttatcaag gaagtcgagt tgaccaaaca
     8101 gcggacgacg ctcggacggc ggccctacaa cgacatcgtg attgagaacc tgacagttag
     8161 cggggagcat ttggccctga acatgcagtc tgatcaagtt acagcggaag atctgaacag
     8221 caccaatggc acatacattg acggcaacgc cattaagttg catgcgttgc gcgacggtga
     8281 catgctggaa gttggcaaat acaagatcag gtttgactcc ggacaagacc gtgacaattt
     8341 cgagaaaacc atgattttca agccatcggc tgcagtccag ccgataccgc ccgtgccagc
     8401 cccggccccg acacctgagt tgcatggctg gatcaaggtg ttgtcaggcg ctgccgccgg
     8461 acgtgaaatg gccttgacca aagtggtcac tacggtcggc aaacccggta tttcagtcgc
     8521 cgccatcacg cagcgtcggc acggtttctt tgtacatcat gtcgagggca atgatcggtt
     8581 gttgcttaac ggcctgccgg ttggcgagga tccttcgcca ctgaggcacg gcgatcagat
     8641 cgcgctgacc ggcacacaaa tgcagtttct gcagcgctga gtgcagttaa cagacaagcc
     8701 aatggcaagc gtcttccata caactttgcc atcatgagct ctgttgtgag atcccatgcc
     8761 gccgagcgtg ctggaatgac cttcgaggcg cttttttaca aacaattgca gcaggtcaca
     8821 gcacgcatcc atgaaactga caacattgag caaatcatgc tcgaagcaag ccaggatatc
     8881 tgcaagctct tcaatgccga cagactgacc ctgtatgtca caaacgaaaa ccaaactgca
     8941 attgtctcca aagtcaaaac cggtttgaac agtagccgtg atctgaagtt gccgatcacg
     9001 ccacaaagca ttgctggcta tgttgcgttc agccacatgc tggtcaattt gcccgatgtt
     9061 tatgacgatg cggcactcaa gaagatccat ccggcgctga ctttcctgaa agaggttgat
     9121 aagcgctcag gttaccggac acggcaaatg ctggtggcgc cgattttgga gggaaacacc
     9181 ctttacggtg tcttgcaggt gatcaacaac aagaacgaca cctcgtttgg tgacttggag
     9241 gtggaaggtg tatcgcagtt gtgcaagaca ctggccactg ccattcgaca ccgcatccac
     9301 gaagcagaag agtcgttgcg gcgcatggtg accaaatatg acggactggt gtccgatggt
     9361 gtcatgacag cggccgagtt gcagcattgt ttgcaagaag cgcgtgagca aggactcaca
     9421 gtcgagcgcg tgttgcttga cacttaccag gttcgtccgg ctcaaatcgg gccgtcgctg
     9481 tccaagtttt ttggtgtgcc ttatgaaccc ttgggcagcg ggcgaatccg ctcggaaatg
     9541 ctgcacggcg cgctcaagcg tgagttcatc gaggagcaag gctggattcc gctggaagag
     9601 tcgcccaacg gactggtcat catgtgtctg gacccggagg cggtgcgcag ttcgcgcatt
     9661 gtgcagcagg tgtttcagcg cgtcaccaaa ttcgattacc gggtcaccac gcacgcggaa
     9721 tttcacgaaa ccctgggtca aatatttggt ctggaagcga gcggcggcag tattgacgaa
     9781 atgctcgctg acatggacag cgcaccgatt gacgacggca gcaacgacga ctcactggag
     9841 tcggctgccg cggacaatga actggtcaag tttgtcaata aggtcattct cgatgcctac
     9901 caccatggcg tgtcggacat ccacattgag ccgatgcccg gcaagctcaa gaccggcatc
     9961 cgatttcgca ttgacggctc gctacaacct tacgctgaag taccggcgca ctttcgacaa
    10021 gccatggtga cacgcctgaa gatcatgtgc gacctcgaca tttccgaacg tcgcaagcct
    10081 caggacggca agatcaagtt caagaaatac ggtccggttg acattgagtt gcgcgtcgca
    10141 accattccat cagcaggcgg ggttgaagat gtggtgatgc ggatcctggc tgccggcgaa
    10201 ccgattccgc tggacaagct gggcttgaca ccgcacaaca aggcgcgggt gatccagacg
    10261 gttgaaaaac catatggctt gttttatgtt tgtggcccta ccggctcggg caaaacaacc
    10321 acgctgcatt ccatcttgaa gcatctcaac aagcctgata ccaagatctg gacggctgaa
    10381 gacccggttg aaatcaccca aaagggcttg cgccaggtgc aaatcaacaa gaaggctggc
    10441 attgacttcg cgctcgtgat gcgggctttt ctgcgagccg acccggacat catcatggtc
    10501 ggcgagtcgc gtgacaagga aaccgtgtcg atgggtgtcg aggcctcgct gacaggtcac
    10561 ctggtttttt ccaccttgca caccaattcg gcacctgagt ccatcacgcg tctgctggac
    10621 atgggcatgg atccctccat tttgccgatg cattgctggg catcctggca caacgacttg
    10681 ccaaacgcct gtgttcctgt aaagagtcat actttccatc cccggaagaa ttgcacctgt
    10741 ttgcgactga gtatgttgaa gaattgcggc actcggcggt ctggaagact gactacgctg
    10801 gccagatgac cgagttgatc gccggttggc aagagctcta tggcgagggc ggaaaaatca
    10861 agctctatcg ccatgtcggg tgcgacaagt gcacccagac tggctacaag ggccgcgttg
    10921 gcctgcacga gttgttgatt gccgacgacg gtatcaaaaa actgattcag gagcgcgccc
    10981 gcgtagccga gattttcgcc gctgcggtcg aaggtggaat gcgtaccctg aaaatggacg
    11041 gtatggaaaa agtcatgatg gggttgactg atctgaagat ggtcagatca gtctgcatca
    11101 agtaatacgg ttcaacctgg attcctccag ttcgatgcac ccaatagatg agtcccatct
    11161 gagctttcga cgtcactgct caaacggact tacagatgac aacaagcggt caactgattc
    11221 atggttcaaa cctttttcag gctcacaggt tgccagcgaa aaggcgacgc atgacacggt
    11281 ctttgaaact gtgcacatcg gccagcccca ggcgctctag gtacgcaggc ggcgcttcct
    11341 tgccttgcca gaggcttttg acggccattg tcagcagttc ggacggatgc tgactggcat
    11401 gtttcatctt tttcatggcg cgcaccatgt gcaactcatc gtcggtcaga tcggttccaa
    11461 acggaaagtc gggtagcagt ccagcttcgg cccagggttt gagcttggct gccagcacct
    11521 caggcaggtt atgccgataa cattcgggca cttcgtagtc cgcttcaagc ttgccgtgcg
    11581 cctgagcctg tttcaccagt tcgccctgaa agcgcgaatc ggcaatggca atcagccgtt
    11641 tgaccacttc accgtcggac cgaccgcgca actcggcaat gccgtattcg gtaatcacca
    11701 cgtcccgcag gtggcgtgga atggttacgt gaccatagtt ccagacaatg ctgcttttca
    11761 gaccgtcctt gttgtcgtgg gtggcacgca gcatcatgat cagccgcgca tcagcgagtg
    11821 catgagacat ggcaacaaag ttgtattgac cgccaacccc gctgaccacc tggccggact
    11881 ccaatgcatc actggctgcg gcacctaaca gggtcacgac catggtggtg ttcatgaacc
    11941 gggcgttgcg acgctgggct cgcttcagcg cgtcattgcc ataaagctgg ttgatgaagt
    12001 caatgcgggt catgtcgatt ttggccagat cctgcggtgc catggtgcgc agacgttcgt
    12061 aaaagtcgcg cggacccaaa aagaagccac cggtcatgaa gatgccgtgc gtcaaccgag
    12121 agcccagcat gtgcttgcta acgcattcaa agctgcccgc agcatgcaag tcgagg
//
LOCUS       DAOSLL010000056        12105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5286_length_12105_cov_4.079751, whole genome shotgun sequence.
ACCESSION   DAOSLL010000056 DAOSLL010000000
VERSION     DAOSLL010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 12105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12105
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5286_length_12105_cov_4.079751"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="PLB25_03880"
     CDS             complement(<1..319)
                     /locus_tag="PLB25_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HOE40757.1"
                     /translation="MSDPTTSPITPATSTATDSGKTSNFLRQIIESDLEKGTYASRHW
                     AGTPGDAAHHAAGQLDPARIRTRFPPEPNGYLHVGHAKSICLNFGLARDYGGVCHMRF
                     DDTN"
     gene            399..1541
                     /locus_tag="PLB25_03885"
     CDS             399..1541
                     /locus_tag="PLB25_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HOE40758.1"
                     /translation="MDETSAISNEPSSASVTRLCLGQYDLDQAAADGLITAQQAHMLW
                     ARWSASHRLTGAAPVLADAGLAAGPRFGFTNTLYYFGGMVAIGAMSLFMTLGWQAFGA
                     WGLATISLAYLLACLAVADHLKIRHLPVPAGILATLAVVLVPLLVWCVQHGLGWWPPG
                     GLSSYSAYHTHINWRWLTLEFATLAAGVVMLWRYRLPFMVMPIAVTLWYMNMDVAHAL
                     WTDNGHWDWKFIRDVSLVFGIATVFIALWVDARCRQAQEPEWRQDYAFWLYLFGTLMF
                     WGGLSLRDSNSELGKAVYCLINLVLVFVGAAIGRRVFTVFGGLGVAIYLGYLSHKVFG
                     DSLMFPFVLTLLGLGLVGLGIWWQRHEVRINSRISAWLPAGLRASF"
     gene            complement(1545..2135)
                     /locus_tag="PLB25_03890"
     CDS             complement(1545..2135)
                     /locus_tag="PLB25_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40759.1"
                     /translation="MSIRYANVINHLPETKMRLYPTKVINIIGGPGCDKSLFSAAIVL
                     YLNLHGKTVETIPDFAKSLVWQQNFEVLKNQYLIAQRQFEMVNLIDGQVHFLITECSL
                     PQVLFYNENYADNICDVAKTREQILEWYRQNDNINILVRRSDKKYVRSGRFQDEEQAR
                     EVDQGLREILDREKMPYTELPPDLKSINAFTEALLA"
     gene            complement(2222..2881)
                     /locus_tag="PLB25_03895"
     CDS             complement(2222..2881)
                     /locus_tag="PLB25_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOE40760.1"
                     /translation="MPETLSLHSLPLFPLGTVLFPGGYLPLQIFEVRYLDMIGKCHKA
                     GAPFGIVTLNRGSELRQAPSNAPGGDSFAQEIFFEVGTLARITDLSRPRPGLMLIQCT
                     GLQRFEVTQHELLKHGLWVADVLVLGDDQRVPVPDDLQPVAATLQRVITTLGLQGTAP
                     DQMPMQPPYLLNDCAWVANRWCELLPMALELKHRLMALDNPLVRLELVSDLLDRNGIP
                     T"
     gene            complement(2888..3511)
                     /locus_tag="PLB25_03900"
     CDS             complement(2888..3511)
                     /locus_tag="PLB25_03900"
                     /inference="COORDINATES: protein motif:HMM:NF013306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HOE40761.1"
                     /translation="MNPFAPPLAEVHLSLRQATKQAHHVLDRHPLLAPLLMSAVSAKQ
                     YGNALSALHGVYDWAERWIDGYLDQYPGEFDYATRRKLPALEADLVALGRHATLARLN
                     LVIPLRRGSLVGVLYTIEGSTMGAQVIARRLRQFAPELPLRFYSGYGDQSRQKWDAFL
                     HFAQTSCPNSEQPAALAASVGLFEAIKTHLDAVLNEITVSEMPVCAL"
     gene            complement(3508..7182)
                     /locus_tag="PLB25_03905"
     CDS             complement(3508..7182)
                     /locus_tag="PLB25_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE40762.1"
                     /translation="MLHKDAAALADALKRCENEPIQHIGSIQSHGALLAIDAHSMVKV
                     VSSNLLEILGLSAKEALNQPAGRILGEAAWVAIATLPPMAPQSQPVPLLLSLFRGEAS
                     LDCQAQVHRADNLLVIEIETPRQTTNLVLPMDSDATDCLLSALLAETDGIDAYSAVIA
                     QQVQALTGFDRVMVYQFDHQWNGKVIAECRKHGVASFLGNHFPASDIPPPARELYTRN
                     LVRTLVDREAPAVALMQAPGLPAGSSLDLSFSVLRSMSPIHLAYLRNLGVRASMSVSL
                     MQNGHLWGLIACHHERPRQLAFKLRQSMELVAKTVAVRLAAIAFGESNHYYATVRNLL
                     PRLAGLHHLAQHGPGDSLLAQDLQQEVLGLVHASGAVVTTGDELTTIGITPLPDQIQA
                     LLDWLRPHLLHEQMYVTHALAQAFAPAAAFSTVASGLLAISLDEGVQHCLLWFREEVV
                     RSVHWAGEATKHLVEDELGPKLEPRRSFERWMQTKRGESPPWTDPEVDAARMLSLTLA
                     ELFSRQQLRVAEESKRLAASVYENSSEAMVVTNASNIILNVNPAFSALTGYSAHEAIG
                     QTPAILSSGQHDRAFYEHMWQSLTETGAWSGEIWNRRKSGEIFPEWLTINSIFDATGA
                     LQQRVALFADITERKRDEANLRIAATAFESQECMTVTNAEGMILRVNQAFTRITGYSA
                     EEVIGQNPRLLHSGRQDPAFYTEMWSRIRQTGSWQGEIWNRHKDGEAYYGWLSITAVR
                     ATGEHVTHYVGTFTDLTDRKEAADKIEHLAFYDHLTELPNRRLMLDRLRQAVVNVNRR
                     QRQGAVMMIDLDNFKSINDTMGHGVGDLLLVETASRLLHSVRAGDSVARLGGDEFVVI
                     LEDLGEGDQAALQAESVGEKVLANLGQAYLLDDGANSQESDSQNHFCTSSIGIALFGD
                     LTASADELLKRADTAMYQSKAAGRNTLRFFDPEMQAAVQARAALEVDLRQAIKEDQFL
                     LHYQAQVDVNGLVLGAEALVRWQHPVRGLISPTEFIPLAEETGLILPLGQWVLDTACH
                     QLSSWAAQTATAHLSLAVNVSARQFGLPNFVEQVLATVELSGARLDRLKLELTESLLL
                     DNADDVVAKMTSLKSHGVFFSLDDFGTGYSSLSYLKRLPLNQLKIDQSFIRDIVSHPN
                     DAAIAKTIVTLGQNLGMTVIAEGVETIGQRNILASLGCLNYQGYYFSRPLPLEGFEAL
                     VNSQTSLPLTAVDLEKQHQP"
     gene            complement(7933..8187)
                     /locus_tag="PLB25_03910"
     CDS             complement(7933..8187)
                     /locus_tag="PLB25_03910"
                     /inference="COORDINATES: protein motif:HMM:NF024776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE40763.1"
                     /translation="MTVTPELHANILRYRLVELWRTGTIAAQLHVHHSTVERLLRQEG
                     LPHVGVVRSSNTDPYLPFILETLKKFPNLRARPWAGEAVI"
     gene            8667..10025
                     /locus_tag="PLB25_03915"
     CDS             8667..10025
                     /locus_tag="PLB25_03915"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOE40764.1"
                     /translation="MQEPDHASESSVDRLYIAAVGPVHADYYLSRFSRFEQSGRAGLS
                     WNWAAGLLTLNWLIFRQLWLQALVYTMAILMLPLLVLGLGRLLLQVPHSVELGLWLAC
                     LVASLVVPGAWGNAVFFGQCKERIAHALETTTTLDAACQRLLLQASNKKRALWLGLVN
                     AAVLGLVAWVYASFAPAPEPEEDQMAVASPVAAFVKPSASARAFAPISAPIAAPVLAS
                     ASAAASASLPSTASASAAAVASSTASAVTAVSSVAAASHFSAPAPASASVTLPVTAPA
                     ALSRPVTAAEPVQASAAGLAASLPVARPAAQLVSRAQQPLSVARTDTPALAASVAKRV
                     LPTDTAAPGRPVASKPAVSKPVASNPVLRRPVKPASGAANGQVFINVGLFADPDNARH
                     AYVKLVKSGLPAERLVLIPAEGSGKAKLTRVRAGPFDSRAQARAAVAQIKALQLDAVL
                     AP"
     gene            complement(10038..10598)
                     /locus_tag="PLB25_03920"
     CDS             complement(10038..10598)
                     /locus_tag="PLB25_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HOE40765.1"
                     /translation="MATKSTIFKASLQIADIDHSYYADHSLTLARHPSETDQRMMVRL
                     CTLALQAHQLQSLCAGDGTLAFGAGLSDPDEPDVWLRDFTGLTRLWIEVGQPEDKPLA
                     KACNKSDSVLLYAFGQAADIWWRAIESKLARLKSLQVWRIPSAEAQQLTALAQRNMNL
                     QATVQEGALLLGDGTHHVHIEPLRWK"
     assembly_gap    10673..10772
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10986..>12105)
                     /gene="purB"
                     /locus_tag="PLB25_03925"
     CDS             complement(10986..>12105)
                     /gene="purB"
                     /locus_tag="PLB25_03925"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOE40766.1"
                     /translation="TNHDVKAVEYWLKGKFEARPELVAVQEFVHFGCTSEDINNTSHA
                     LQLKCGRDQVLLPALDVIIDKLREMAHAFADVAMLSRTHGQTASPTTVGKEIANVVVR
                     LEVARVKIANVKLMAKMNGAVGNYNAHLSAWPEHDWETFSKKVIETPEPLGLGLTFQP
                     YSIQIEPHDYMAELFDAVARTNTILIDWSRDVWGYVSLGYFKQKLRKGEVGSSTMPHK
                     VNPIDFENAEGNLGLANALLRHLSEKLPISRFQRDLTDSTVLRNMGVALGYAVLAYQS
                     LTAGLGKLEINEAAIASDLDDSWEILAEPIQTVMRRFGLPEPYEQLKEFTRGAAMSAE
                     LMQGFVAKLAIPEAEKTRLLALTPGGYTGKAAELARRV"
BASE COUNT         2305 a         3394 c         3672 g         2634 t
ORIGIN      
        1 ggttggtgtc gtcaaagcgc atgtggcaca cgccaccgta gtcgcgcgcc aggccaaagt
       61 tcaggcaaat gcttttggcg tgcccgacat gcaagtagcc attgggctcg ggtggaaagc
      121 gggtgcggat tctggccgga tccagttgac cggcggcgtg gtgggcagcg tcgccaggcg
      181 tgccggccca atggcggctg gcataggtgc ctttttccag atcagattca atgatctggc
      241 gcagaaaatt gctggtcttg ccagagtcag tggccgtgct tgtggctggc gtgatggggg
      301 aggtagtagg gtcgctcatt cggttgattt taagggcccc gcgggttcgc tatgatttgc
      361 gccaactgtc caagctttca cacctcaaag aacctgccat ggatgaaact tcagcgattt
      421 cgaacgagcc ctcaagtgcc tcagtgaccc ggttgtgcct ggggcagtac gacctggatc
      481 aggccgcggc cgacggtttg atcaccgcac agcaggcgca catgttgtgg gcacgctggt
      541 cagcatcgca tcggctgaca ggggctgcac ccgtgttagc cgatgccggt ttggcggccg
      601 gtccgcgttt tgggtttacc aataccttgt actattttgg tggcatggtg gcgattggtg
      661 ccatgagctt gttcatgacg ttgggctggc aggcttttgg agcttggggt ctggctacca
      721 ttagcctagc ttatctattg gcctgtctgg cggtggcaga ccacctgaag atccggcact
      781 tgccagttcc ggcaggcatt ctggcaacgc tggcagtagt gctggtgccc ctgctggtgt
      841 ggtgcgtgca gcatggcctg ggttggtggc caccgggtgg tttgagcagt tattccgcct
      901 accacaccca catcaactgg cgctggctga cgctcgaatt tgccacgctc gccgctgggg
      961 tggtgatgtt gtggcgctac cgcctgcctt tcatggtgat gccgattgct gtaacgctgt
     1021 ggtacatgaa catggatgtt gcccacgcgc tgtggactga taacggccac tgggactgga
     1081 agttcatccg cgatgtgtca ctggtgtttg gcatcgccac cgttttcatt gcgctgtggg
     1141 tggatgcacg ttgccgtcaa gcacaggagc ctgagtggcg ccaggactac gctttttggc
     1201 tgtacctgtt tggtacgttg atgttttggg gtgggctgag cctgcgcgac tccaattccg
     1261 agctgggcaa ggcagtttac tgcctgatca atctggtgct ggtgtttgtc ggggccgcca
     1321 ttgggcggcg tgtgttcact gtgttcggtg gcctcggggt ggccatttac cttggctacc
     1381 tgtcgcacaa ggtatttggc gacagtctga tgtttccgtt tgtgctgacg ttgctgggct
     1441 tgggcctggt ggggctgggc atctggtggc agcgccatga agtgcgcatc aacagccgca
     1501 tcagcgcctg gttacccgcg ggcctgcgag cgtctttttg atgatcacgc caacaaggct
     1561 tcggtaaaag cattgatgga tttcaggtcc ggtggaagtt cggtgtaggg cattttttca
     1621 cggtccagaa tctcccgcag tccctggtcc acctcacgtg cctgttcttc gtcctgaaag
     1681 cggccggagc gcacatattt tttgtcactg cgcctgacca gaatattgat gttgtcgttc
     1741 tgccggtacc actccagaat ttgctcgcgt gttttggcta catcgcagat gttgtcggcg
     1801 tagttttcgt tgtaaaaaag tacctgcggc aatgaacact cggtgatcag aaaatgcacc
     1861 tggccgtcga tcaggttgac catttcaaat tgccgctggg cgattaagta ctggtttttc
     1921 agtacctcga aattttgttg ccaaaccagc gacttggcaa agtcgggaat ggtttcaacg
     1981 gttttgccat gcaggttcag atacagcacg atggccgctg aaaacagcga tttgtcgcag
     2041 cccggcccgc caatgatgtt aatgaccttg gtggggtaca gccgcatctt ggtttcgggc
     2101 aagtggttga tgacgttggc gtaacggatg ctcatggtgt tctaccggtg gtttgacgat
     2161 ccagcgttca tagtctgcca aacttggcca atttgcaagc cgttttttgc atgcgtcaaa
     2221 actaggtcgg aataccgttg cggtcgagca ggtcgctgac caattccagt cgaaccagcg
     2281 ggttgtcaag cgccatcagg cggtgcttga gttccagcgc catcggcagc agttcgcacc
     2341 agcggttggc cacccaggcg caatcattga gcaagtaggg cggttgcatt ggcatctggt
     2401 ccggtgctgt gccttgcaac ccaagcgtgg taatcacgcg ttgcaaggtg gcagcgaccg
     2461 gctgcaaatc gtcgggaacc ggcacgcgct ggtcatctcc gagcaccagc acatcggcca
     2521 cccacaaacc gtgcttgagc agttcatgtt gcgtcacctc aaaacgctgc aagccggtgc
     2581 attggatcaa catcaggccc ggtcggggtc ggctcaggtc ggtgatgcgg gccaatgtgc
     2641 caacttcaaa aaaaatctcc tgggcaaaac tgtcaccgcc tggtgcattg ctgggggctt
     2701 ggcgcaactc gctgccccgg ttcagggtga cgatgccaaa aggtgcgccg gccttgtggc
     2761 acttaccaat catgtcgagg tagcgcacct caaaaatctg cagcggcaaa tagccgccgg
     2821 gaaacagcac ggtgcccagt ggaaacaggg gcaatgaatg gagcgaaagg gtctcgggca
     2881 tgtagtctca aagcgcacaa acgggcattt cagaaacggt gatttcattt aaaaccgcat
     2941 ccaggtgagt cttgatcgcc tcaaagagcc cgaccgatgc tgccagcgcc gcgggctgtt
     3001 cgctgttggg acaactggtt tgggcaaagt gcaaaaacgc atcccatttt tggcgtgatt
     3061 gatcaccata gcctgaataa aatcgcaatg gcaattcggg cgcaaactgg cgcaatcgcc
     3121 gtgcgatcac ctgtgcgccc atggtggaac cctctatggt gtaaagaacg ccaaccagac
     3181 tgccccggcg caacggtatc accaggttca atcgggccag cgtagcgtgt cgccccaatg
     3241 cgaccagatc tgcctccagg gcgggcaact tgcggcgtgt ggcgtagtca aattccccgg
     3301 gatactggtc aagatacccg tcaatccagc gctcggccca gtcgtagaca ccgtgcaatg
     3361 ccgacagtgc attgccgtac tgtttggcag acactgctga catcaatagt ggtgcaagca
     3421 aggggtggcg atcaagcaca tgatgggctt gcttggtggc ttgccgaaga ctaaggtgca
     3481 cttcggcaag agggggcgca aatgggttca tggctggtgt tgtttctcta agtccacggc
     3541 agtcagaggc aggctggtct ggctattgac cagggcttca aatccttcca gtggcaacgg
     3601 gcggctgaaa taataccctt ggtagttcag gcaacccagg ctggccagaa tgttgcgctg
     3661 accaatcgtt tcgaccccct cggcaatcac cgtcatgccc aagttttggc ccaatgtcac
     3721 gatggttttg gcgattgccg catcgttggg gtgggatacg atgtcgcgaa taaaagactg
     3781 gtcaatctta agctgattca gtggcaggcg tttcaggtac gacagcgacg agtagccggt
     3841 gccgaagtca tccagagaaa aaaacacccc gtgcgacttc agactggtca ttttggcaac
     3901 aacatcgtcg gcgttgtcga gcagcaggct ctcggtgagt tcgagtttga gtcggtccag
     3961 gcgggcaccg ctgagctcaa cggttgccaa aacctgctcg acaaagtttg gcaagccaaa
     4021 ttgacgagcg ctgacattca ccgccagact caggtgcgcg gtggcagtct gggcagccca
     4081 gcttgagagc tggtgacagg cggtatcgag cacccattgc ccgagtggca ggatcagccc
     4141 ggtttcttca gccagcggga taaattcggt tggcgatatc agtccgcgca ccgggtgctg
     4201 ccagcgaacc agcgcctcgg caccaagcac cagaccattc acgtcaacct gtgcctggta
     4261 gtgcagcagg aactgatctt ccttgatggc ttgacgcagg tccacttcaa gggccgcgcg
     4321 ggcttgtacg gcagcctgca tctccggatc aaaaaagcgc aacgtattgc gtccggccgc
     4381 tttggactgg tacatcgcgg tgtcggcccg tttgagcagt tcatcggccg atgccgtcag
     4441 gtcgccaaac aacgcgatac cgatgcttga ggtgcagaaa tggttctggc tgtccgactc
     4501 ctggctattc gcgccatcat cgagcaggta ggcttggccc agattggcca acactttctc
     4561 gcccacagac tcagcttgca aggccgcctg atcaccttcg cccaggtctt ccaagatcac
     4621 aacaaattcg tcgccgccca aacgtgctac agaatcacct gcgcgaacgc tatgcagcag
     4681 gcgcgatgct gtctcaacca gcagcaagtc gcccacccca tggcccatgg tgtcgttgat
     4741 agacttgaaa ttgtccaggt caatcatcat caccgcacct tggcgctggc gtcggttgac
     4801 attgaccaca gcttggcgca gtcggtcgag catcaggcgg cggttgggta attcggtcag
     4861 atggtcgtag aaagccagat gctcgatttt gtcggcagcc tccttgcggt cggtcaagtc
     4921 ggtaaaggtg ccgacatagt gcgtcacgtg ctcgccggtg gccctgactg cggtgatcga
     4981 cagccagccg taataggctt cgccgtcctt gtgccggttc cagatttcgc cctgccagga
     5041 gccggtttgc ctgatgcgcg accacatttc cgtgtaaaag gccggatcct gacgaccgga
     5101 atggagcaat cgcgggtttt ggccaatcac ctcttcggcg ctgtagccgg taatgcgggt
     5161 gaaagcctgg ttgacccgca ggatcatgcc ctcagcattg gtaacggtca tgcattcctg
     5221 cgactcaaag gcggtagcgg caatgcgcag gttggcttcg tcgcgtttgc gttcggtgat
     5281 gtcggcaaac agtgccaccc gttgctgcag cgcgccagtc gcatcaaaaa tgctgttgat
     5341 ggtcaaccac tcgggaaaaa tctcaccgct cttgcggcgg ttccagattt cgccgctcca
     5401 ggcccctgtc tctgtcagcg actgccacat gtgctcatag aacgcccgat catgctgacc
     5461 ggaactcaaa atggccgggg tctgaccaat cgcctcatgc gcactgtagc cggtcagcgc
     5521 gctgaaggcc ggattgacgt tgagaatgat gttgctggca ttggtcacca ccatcgcttc
     5581 gctgctgttt tcatagaccg aggcggccag ccgcttggac tcttcggcaa ccctgagctg
     5641 ctgcctggag aatagctcgg ccagtgtcag cgacaacatg cgtgccgcgt ccacctcggg
     5701 gtcggtccac ggtggcgact caccacgctt ggtttgcatc cagcgttcaa aggatctgcg
     5761 cggttcaagc ttgggaccaa gctcgtcttc gaccagatgc ttggtcgctt cgccagccca
     5821 atgcaccgag cggaccacct cttcacgaaa ccaaagcagg caatgctgta cgccctcgtc
     5881 cagactgatt gccagcagcc ccgaggccac tgtcgagaag gcggccgcag gcgcaaaagc
     5941 ctgcgccagc gcatgcgtga catacatctg ctcatgcaac aaatgcggtc gcaaccagtc
     6001 gagcagcgcc tgaatttgat caggcaacgg tgtgatgccg atggtagtca actcatcgcc
     6061 ggtggtcacc acagcaccgc tggcatgcac caggcccagc acttcctgct gcaaatcttg
     6121 cgccagcaac gaatcgccgg ggccatgctg cgccagatgg tgcagaccag ccagccgcgg
     6181 tagcaggttg cgcaccgttg cgtagtaatg attgctttcc ccgaaggcga tggccgccag
     6241 ccgcaccgcg acggtcttgg ccaccagctc catgctctgg cgcagcttga aggcaagttg
     6301 ccgggggcgc tcgtggtggc aggcaatcag cccccacaga tggccgtttt gcatcagtga
     6361 caccgacatt gaagcccgca cgcccagatt gcgcaggtat gcaaggtgaa tcggcgacat
     6421 gctgcgtagc accgaaaacg acagatccag tgaagacccg gctggcagtc cgggcgcttg
     6481 catcaaggct accgctgggg cctcccggtc caccaaggtg cgcaccagat tgcgggtgta
     6541 aagctcgcgt gccggcggtg gtatgtcgga ggccggaaaa tgattcccca gaaacgacgc
     6601 gacaccgtgt ttgcggcatt cggcgattac cttgccgttc cactggtggt caaactggta
     6661 caccatgacc cggtcaaagc cggtcagtgc ctgcacctgt tgggcgatca ctgccgaata
     6721 ggcatcgatt ccgtcggtct cggccagcag ggcgctgagc aaacagtcgg ttgcatcgga
     6781 atccatcggg agcaccaagt tggtggtctg gcgtggggtt tcaatttcaa tgaccagcaa
     6841 gttgtcagcg cggtgcacct gcgcctggca gtccaggctg gcttcacctc gaaacaagct
     6901 gagtaaaagg gggactggtt ggctctgcgg ggccatcggc ggcaacgtgg caatggcgac
     6961 ccaggccgct tcgcccagaa ttctgccagc gggctggttc aatgcctctt tagccgacag
     7021 gccgagaatc tcaagcaggt tactgctgac caccttaacc atggaatggg catcgatagc
     7081 cagcagtgca ccgtggctct gaatcgaacc aatgtgctga atgggttcgt tttcgcaacg
     7141 cttgagcgca tcggccaaag ccgctgcgtc tttatgcaac atggtttgtt ccaattgatt
     7201 gtctcgttga ggttcgacca gcaccaaacc tacccaagac caaactgatc gacaggccag
     7261 attgtccgcc tttttgcggc aaaaacccaa cttggtgcag cggcggcaga ttgtggtgca
     7321 ggactttgat ggccgtggcg tggctttcct gtcggcgttg cctgcatcac atggcatcgc
     7381 tggggaacca acggcacaag ttgctgcgga cagcgctagc aatcaattga ctcaaatccg
     7441 attgtcacct gtcggatgga aattttcggc cggactgttt gggttacttg atttgggaat
     7501 gcatgcggac ttcggcgcag gcggtgtttt ctgctgcgga gaccattggt gtccgcgccg
     7561 attgagtatt gacccaccct gccgatccaa tattgaccca ggaccggtag ctgcctttta
     7621 aactgaagtt ccatccggat ttggttggat tcctattgaa atcaacactt tagtgcaatt
     7681 ttgagtgcca gtatgtagac atgtatttaa tagattatta atcttaatat gttatacata
     7741 tgtagacatg cacataaata tcgtcccaga tcgtggctcc gcctccaccg ttttgctgcg
     7801 tgagtcctac cgtgatggct ccaaggtttg caagcgcacc ctggccaact tgtcggcgtt
     7861 gtctgccgca caagtgcaga tgattcgtgc caccttgcgt ggcgatgtgt tgcagccagt
     7921 tgatcaaacc tttcagatca cagcctctcc tgcccacggt ctggctctga ggttcgggaa
     7981 cttcttgagt gtttccagaa tgaacggcag ataggggtca gtgttggaac tgcgcaccac
     8041 ccccacgtgt ggcagcccct cctggcgcag gaggcgctcg acggtgctat ggtggacgtg
     8101 caattgggcc gcaatggtgc cggtgcgcca aagctcaacc aggcgataac gcaatatgtt
     8161 ggcgtgtagt tcaggagtca cggtcatggt ggggtccttg tctggtctgg caaacgggtg
     8221 gggcgacgga tgcgacagcg caaaaagtca gttcgcacaa actcagtgca ctcgcactgg
     8281 cagaaatgac accgccagag cgactttgag gcttccaagg cttggctttg agggctatgc
     8341 agcgctgtcg tctcgctggg cagtagagca gcaaggcttg gaggtgggca accctgatgc
     8401 gtcacttttt ccctgatggc caccccaaac tgctgcactt atggcccccc aaactgttcc
     8461 aggcgggacg gcagggcgtt gcgcgcatcg ggtcgattca gggtcaagat aaccggaccg
     8521 cgcgcgtctc agacgatcaa gactagcggt gtcatgatgg tgttcatttg gtgggtgccc
     8581 tgaacttgat gaatgccgtc aggcatcgct gagacagtgc tgcggcatgc aaaaatgcgg
     8641 caatgaccga aaagctggta ccgcacatgc aagaacctga tcacgcttcc gaaagctcgg
     8701 tggaccggct gtacatcgca gcggtcggac cggtccatgc agactattac ctgtcacgct
     8761 tttcccgctt tgagcagtct ggtcgggctg gcttgtcatg gaactgggcg gctggcctct
     8821 tgacgctgaa ttggctgata tttcgccagc tttggttgca ggcattggtt tacaccatgg
     8881 ctatattgat gctgccgctg ctggtgctgg gactggggcg cctgctgtta caggtgcccc
     8941 acagtgtcga gctggggttg tggctggcct gtctggtggc cagtctggtg gtgccgggtg
     9001 cctggggcaa cgcggtgttc tttggccagt gcaaagagcg cattgcccat gcgctggaaa
     9061 ccaccacgac gctggatgcg gcttgtcagc gcctgctgtt gcaggccagc aacaaaaagc
     9121 gggcgctctg gctggggttg gtcaatgccg ctgtgctggg tttggtggcc tgggtctatg
     9181 ccagttttgc accggcgccg gaaccggaag aagatcagat ggccgtggct agcccggttg
     9241 ccgcttttgt caagccgtct gcgtcggcgc gagcgtttgc gccgatatct gcgccgatag
     9301 ctgcgcctgt gcttgcatcg gcctctgcgg ctgcatctgc gtcgttgccc tcaactgcgt
     9361 cagcctctgc ggctgctgtc gcatcgtcaa cggcatcggc tgtcaccgct gtgtcatctg
     9421 tggccgctgc cagtcacttc tctgcgccgg caccagcatc ggcatctgtc actttgcccg
     9481 taaccgcgcc cgctgccttg tcaagaccgg ttacggcggc tgagccagtt caggcatcag
     9541 cagccggatt ggcagcaagt ttgcccgtgg cccgcccagc cgctcagttg gtaagccggg
     9601 cccagcagcc gctgtctgtc gctcgaacgg ataccccggc cttggcggca tcggtggcaa
     9661 aacgggtatt gccgactgac actgctgcgc caggcaggcc ggtggccagt aaaccggccg
     9721 tcagcaagcc agtcgccagc aatccagtgc tgcgcagacc ggtgaaacca gcttcaggtg
     9781 cggcaaatgg tcaggttttc atcaatgtcg gtttatttgc tgaccctgat aacgcacgcc
     9841 atgcctacgt caaactggtg aagtccggtt tgcctgccga gcgcctggtc ctgataccgg
     9901 ctgaaggctc tggcaaagcc aaactgaccc gggtgcgggc cgggccgttt gacagccggg
     9961 cgcaggcgcg cgctgcggtg gctcaaatca aggccttgca gcttgatgcc gtgctggcac
    10021 cctgatgcgg ctggcgacta tttccagcgc agcggttcaa tgtgaacatg gtgggtgccg
    10081 tcacccagca gcagcgcgcc ttcctgcacg gtcgcctgca ggttcatgtt gcgctgcgca
    10141 agtgccgtca actgctgcgc ttcggcactc ggaatgcgcc agacctgcag gcttttgagc
    10201 cgggcaagtt tgctctcgat ggcgcgccac cagatgtctg cggcctggcc aaaagcatag
    10261 agcagcacgc tgtcagactt gttgcaggcc ttggcaagcg gcttgtcttc gggctgaccg
    10321 acttcaatcc agagccgggt caggccggtg aagtcgcgca gccagacatc gggctcgtcc
    10381 gggtctgaca ggcctgcgcc aaacgccagc gtgccgtcac cagcgcagag gctttgcaat
    10441 tgatgcgcct gcaatgccag ggtacaaagc cgcaccatca tccgttgatc ggtttcgctc
    10501 gggtggcgtg ccagcgtcag gctgtggtcg gcgtaataac tgtggtcaat atcagcaatt
    10561 tgcaggctgg ctttgaagat ggtggatttg gtggccatta aacctgccgc cgggccgggg
    10621 tgagccccct cggggggcag agaggacacg cagtgccgag cgtgggggtc atnnnnnnnn
    10681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngggtgagc cccctcgggg ggcagagagg
    10801 acacgcagtg ccgagcgtgg gggtcatgac ctgccgccgg gccgccccaa ggcgggtgag
    10861 ccccctcggg gggcagagag gacacgcagt gccgagcgtg ggggtcatga cctgccgccg
    10921 ggccgcccca aggcgggtga gccccctcgg ggggcagaga ggacacgcag tgccgagcgt
    10981 gggggtcata cccgccgcgc cagttcggcg gccttgccgg tgtagccgcc cggagtcagg
    11041 gccagcagac gggttttttc tgcttcggga atcgccagct tggcgacaaa accctgcatc
    11101 agttcggcac tcatggccgc accccgggtg aattccttga gctgttcata aggctcgggc
    11161 aagccaaagc ggcgcatcac ggtctggatc ggctcggcca gaatctccca tgagtcgtcg
    11221 agatcgctgg caatcgccgc ttcgttgatt tccagtttgc caaggcccgc cgtcagcgac
    11281 tggtaggcca gcacggcgta gcctagcgcc acacccatgt tgcgcagcac ggtgctgtcg
    11341 gtcaggtcgc gctgaaagcg gctgatgggc agcttttcac tcaggtgacg caacagcgcg
    11401 ttggccagac caaggttgcc ctcggcattc tcgaagtcaa tcgggttgac cttgtgcggc
    11461 atggtgctcg aacccacttc acccttgcgc agcttctgtt tgaaatagcc caacgacaca
    11521 taaccccaaa cgtcgcgcga ccagtcgatc aggatggtgt tggtgcgtgc caccgcatca
    11581 aacagttcag ccatgtagtc gtgcggctcg atctggatgc tgtagggctg aaacgtcagg
    11641 cccaggccga gtggctcggg ggtttcgatc acttttttgc tgaaggtttc ccagtcgtgc
    11701 tcgggccagg ccgacaggtg ggcgttgtag ttgccaaccg caccgttcat tttggccatc
    11761 agtttgacgt tggcgatctt gacgcgcgcg acctccaggc gcaccaccac attggcgatt
    11821 tccttgccca ccgtggtcgg gctggcagtt tgcccatggg tgcggctgag catggccacg
    11881 tcggcaaacg catgcgccat ttcgcgcagc ttgtcgatga tgacatcaag tgctggcagc
    11941 agcacctgat cacgcccgca tttgagctgc agtgcatggc tggtgttgtt gatgtcttcg
    12001 ctggtgcagc caaaatgcac aaattcctgc accgccacca gttccggacg tgcctcgaac
    12061 ttgcctttga gccagtactc aaccgctttc acgtcgtggt tggtg
//
LOCUS       DAOSLL010000057        12004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5336_length_12004_cov_4.646247, whole genome shotgun sequence.
ACCESSION   DAOSLL010000057 DAOSLL010000000
VERSION     DAOSLL010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 12004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12004
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5336_length_12004_cov_4.646247"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(236..1144)
                     /locus_tag="PLB25_03930"
     CDS             complement(236..1144)
                     /locus_tag="PLB25_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE40767.1"
                     /translation="MYQAQRLSRSEFVPVRTLNYHVRIWGKPVPGAAPLVLLHGWMDV
                     AASFQFVVDALAAEHFIIAPDWRGFGLTDSGGVDCFWYQDYLADLDALLDHYASDMPV
                     NLVGHSMGANVAMLYAGVRPKRVRRLINLEGFGLPATQAVQATAHYGQWMDQLRQLHT
                     GHLHVKTYASLKAVAARLMKTNKRLSPDKADWLAGHWSAELSPGRWSILGEVAHRVVS
                     AQLYREDETLVIFACIAAPVLMVEASDDSMAVWWKGKFTLAQHHERLKAVPRLVRVTL
                     PDCSHMLHHDQPELLADLMERFLSQS"
     gene            complement(1259..3145)
                     /locus_tag="PLB25_03935"
     CDS             complement(1259..3145)
                     /locus_tag="PLB25_03935"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE40768.1"
                     /translation="MTDATLATDDFLSVEIISNDINVLPNDSIDTPALAEAAAPEPEV
                     PNGFIALGLAPELIRAVKDLGFVQPTIVQQKTIPLAMQGHSGGVKDARFIDLMVSSQT
                     GSGKTAAFLLPVLHTLLTQQADAETKEREDYQRAVADAAAKGEAPPKRAKRKDPTNPR
                     HFTAPTPGALVVCPTRELAQQVAHDAIDLVQHCRGLRIANIVGGMPYQLQIAKLQNAN
                     LVVATPGRLLDLQRSMQIKLDQVQFLVVDEADRMLDLGFSDDLAEINQLTITRKQTMM
                     FSATFAPRIQQLAARVMREPQRITIDNPQEKHANIKQVLFWADNAQHKRKLLDHWLRD
                     TGINQAIVFASTQIECDGLANDLQQDGFDAVALHGALSQGLRNRRLMALRQGQVQILV
                     ATDVAARGLDVPTVTHVFNFGLPMKAEDYTHRIGRTGRAGRDGLAVTFAEFRDRRKIF
                     DIEAFTRQPIKAEVVPGLEPQQRMPESRPNSGFGGRDSRGGSDFQSRDRKFGGGGRPS
                     GAGGFGGNRGGFSDNRGGFGDRNAAGPRVMGADSYQNNNGGGFAQRDERPAVPAREGF
                     SYERKGGFNDRFAGDRSAGAAPRGDFAPRKPAFGKPAFSKPAGGGKVFVPRDAQKRFS
                     KNDR"
     gene            complement(3712..4158)
                     /locus_tag="PLB25_03940"
     CDS             complement(3712..4158)
                     /locus_tag="PLB25_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOE40769.1"
                     /translation="MTTGTVQLHRVLRTPPERVYRAFLDADAMAKWIPPYGFTCKVHQ
                     MDARVGGTFRMSFTNFTSGNRLSFGGEYLELVPHQTIRYTDKFDDPNLPGEMQVTVAL
                     AKVSCGTEISIVQEGIPAMIPVEMCYLGWQESLAQLATLVEPEIPG"
     gene            complement(4332..5117)
                     /locus_tag="PLB25_03945"
     CDS             complement(4332..5117)
                     /locus_tag="PLB25_03945"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HOE40770.1"
                     /translation="MKGGGIFGSTIDLSTELFKPAGPGPFPVLVYAHGRSGTQQERSA
                     LAEVIPRQFLSFWLARGFAVVAPARPGYGKTGGNDREVPGHSWDGRGNCSGVPNAERV
                     AATASAAIMAAVDWVREQPWAISSRILVAGNSVGGLTSAALASANPPGVVAYINFAGG
                     IAGNPDQSPGKSCAPEKVREAFVLYGKTARVPSLWLYAQNDKYWGPDTPGQWHAAYVA
                     SAGIAEFVAMPPVPNSDGHNLIFVGRDLWAPVVESFLKRVGFE"
     gene            complement(5953..6816)
                     /locus_tag="PLB25_03950"
     CDS             complement(5953..6816)
                     /locus_tag="PLB25_03950"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HOE40771.1"
                     /translation="MSVLPTDRHAASLQRAAELIAGADALVIGAGAGIGVDSGLPDFR
                     GSEGFWQAYPALARAQLDFTEVANPDMFASDAGLAWGFYGHRLGLYRQTVPHAGFDML
                     RQWGQRMRLGSWVFTSNVDGQFQKAGFAEELIHECHGSIHHLQCLQDCQSDVWSAAGF
                     TPVVDNDTCRLLNDPPVCPHCGGLARPNILMFGDWLYQHRRGQAQHRRMDQWLDSLSQ
                     HAATPVIVELGAGTAIPSVRHFSQQVSRRYGARIIRINPREPEVPGSQDVGLACGALA
                     GLSGIDALLKA"
     gene            complement(6916..7506)
                     /gene="orn"
                     /locus_tag="PLB25_03955"
     CDS             complement(6916..7506)
                     /gene="orn"
                     /locus_tag="PLB25_03955"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HOE40772.1"
                     /translation="MSTPNTPTNTASLAKSDQNLVWLDCEMTGLEPETERILEIAVIV
                     TGPKLEPRIEGPVLVVHQSDELLAAMDKWNQSTHSKSGLIDRVRASTLTEEQAQEQIL
                     AFLKHYVPKSGSPMCGNTIGQDRRFLAKYMPRLEAFFHYRNLDVSTLKELAKRWAPDV
                     YKGFKKKQRHTALADVYESIDELEHYREHLLKLPVS"
     gene            7545..8840
                     /locus_tag="PLB25_03960"
     CDS             7545..8840
                     /locus_tag="PLB25_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOE40773.1"
                     /translation="MIETKLSSDPSALLSMTFAVALAVSVLLKLWLASRQVRHVALHR
                     DQVPAAFAQTISLAAHQKAADYTLAKARLGFLELPLNLIVLLGFTLLGGLSALNDLLV
                     QWLGTGLLQQLVLLVSFSLITGLIDLPLSLYQTFVLEQRFGFNKMTLGLWLADLLKSS
                     LMGALIGLPIAALILWLMAASGGLWWLWAWCVWMGFNLLLLVIYPTLIAPIFNKFTAL
                     EDATLKARVTALMQRCGFSAKGLFVMDGSKRSAHANAYFTGFGAAKRVVFYDTLLSKL
                     SPAEVDAVLAHELGHFKHRHIIKRIVSMFAISLAGFALLGWLAQQAWFYTGLGVMPNL
                     TGPNDALALLLFMLAMPVFGFFVAPVMAHFSRKHEFEADAYAMAQTSGPDLASALLKL
                     YEDNAATLTPDPLYVAVYYSHPGASERLARMHPIESALA"
     gene            8900..9223
                     /locus_tag="PLB25_03965"
     CDS             8900..9223
                     /locus_tag="PLB25_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HOE40774.1"
                     /translation="MPQALTRRALTATEVITSLAKLEGWRLSGDGAAVAIEKSFAFAD
                     FYQTMAFVNALAFIAHGQDHHPELIVQFRECLVRWRTHDLGGLSKSDFDCAARVEALL
                     ANARG"
     gene            9275..10222
                     /gene="rsgA"
                     /locus_tag="PLB25_03970"
     CDS             9275..10222
                     /gene="rsgA"
                     /locus_tag="PLB25_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HOE40775.1"
                     /translation="MKINARATIAANTRHQSGLIVANFGRHFLVENAAGERLICHSRG
                     KKSQGVVGDKVLWQASEDEGTIEKIEARRNLFYRQDEVRTKSFAANLDQVLILIAAEP
                     EFSSSQLSRALIAAEEQHITPIIALNKSDLTEPFSRAWHWLEPYRQMGYDVLALSLAA
                     GSQADRDALTQRLRGKTTLVLGPSGAGKSTLINCLIPGALVQTGELSQALSSGKHTTT
                     STTWYWVDAAKTTALLDSPGFQEFGLNHIDPAQLAAYMPDLKPHVAQCKFYNCSHLHE
                     PGCGVISMVKSGASADGISANRYKIYSDLYAELKQTRRY"
     gene            complement(10750..11820)
                     /gene="hisC"
                     /locus_tag="PLB25_03975"
     CDS             complement(10750..11820)
                     /gene="hisC"
                     /locus_tag="PLB25_03975"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HOE40776.1"
                     /translation="MSLSFFSPVLARLVPYVPGEQPKIDKLVKLNTNENPYPPSPRVV
                     LAITRAAQQGLQLYPDPDASALRQAIAAHHGIAEKHVFLGNGSDEVLAHAFFAFFQQG
                     LPLLMPDISYSFYQVYCRLYSIAAQVVPLTEDLRLNVADYACADGLKVAGVVLANPNA
                     PTGVGLPLADIETLLQMHPKRVVLVDEAYVDFGGQSALELIGRYPNLLVVHTLSKSRS
                     LAGLRIGYACAQPHLIEALQRVKNSFNSYPLDRLAIAGAVAAFEDQGYFEQTRQAVMS
                     SREGLVLALEDLGFVVLPSQANFVFARHRSRDAAALAADLRARGVLVRHFSLPRIDQY
                     LRISVGTPQQCDLLIQAISSSF"
     gene            complement(11894..>12004)
                     /locus_tag="PLB25_03980"
     CDS             complement(11894..>12004)
                     /locus_tag="PLB25_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiB"
                     /protein_id="HOE40777.1"
                     /translation="LLPELQHLAVVVGLVWRTVVMWIVLLALLTLARLLG"
BASE COUNT         2462 a         3607 c         3517 g         2418 t
ORIGIN      
        1 tatcatgcga agaattcaaa cattcaaagt cattcgccgc gagggcgcga ctcctgcaaa
       61 ttcaaatagg tgccgactgt ttgtgtagga gtcgcgccct cgcgacgaag gctcatcgat
      121 tccggctcgt ccggcttagg atgatgttga acgggtttgc aagcccggaa tacatccgaa
      181 gtacatgaac cgacctgaaa cctgatgcca ggggggtttg ttgttgcacc cggttttacg
      241 actgcgacaa gaaccgctcc atcagatccg ccagcagttc gggctggtca tgatgcagca
      301 tgtgactgca gtcaggcagg gtgacacgca caagccgggg caccgccttg agtcgttcgt
      361 ggtgctgcgc cagggtgaac ttgcccttcc accagaccgc catgctgtcg tcagaggctt
      421 cgaccatcag caccggcgcg gcgatacagg caaagatgac cagcgtttcg tcttcacggt
      481 aaagctgcgc cgacaccacc cggtgcgcca cctctcccaa aatcgaccag cgcccgggcg
      541 acagctcggc cgaccagtgg cccgccagcc aatcggcctt gtcggggctc aggcgcttgt
      601 tggttttcat cagccgcgcg gcgacggctt tgagcgaggc ataggttttg acatgcagat
      661 ggccagtgtg cagttgcctg agctggtcca tccactgccc ataatgggcc gtggcctgca
      721 ccgcctgggt ggcgggcagg ccaaaacctt caagattgat caggcggcgc acccgcttcg
      781 ggcgcacgcc ggcatacagc attgccacat tggcacccat gctgtggccg accaggttga
      841 ccggcatgtc gctggcgtag tggtccagca atgcgtccag atcggccagg taatcctggt
      901 accagaagca gtcaacaccg ccagagtcgg tcaggccaaa accacgccag tccggggcga
      961 tgatgaaatg ctcggccgcc agcgcatcga ccacaaactg aaagctcgct gccacgtcca
     1021 tccagccgtg caacagcacc aaaggtgctg cgccgggcac tggcttgccc cagattctga
     1081 cgtggtaatt gagtgtgcga accgggacaa attcgctacg ggacaggcgt tgggcttggt
     1141 acatgtgcca catgttacga aagacccacc aggcctgtca gtcaaaccag cgacaccgcc
     1201 cggacatctg caggctgctt gtcaacgcct gcaggtttgc aggcatccca gcgcaaattc
     1261 aacggtcgtt cttggagaac cgcttttgcg cgtcacgcgg tacaaacacc ttgccgccac
     1321 cggctggttt ggaaaatgct ggcttgccaa aggccggctt gcgtggcgcg aaatcaccgc
     1381 gcggtgcggc acctgcactg cggtcaccgg cgaagcggtc gttaaagcca cccttgcgct
     1441 cgtagctgaa accctcgcgc gctggtactg cggggcgttc gtcgcgttgc gcaaatccac
     1501 caccgttatt attttgatag ctgtcagcgc ccatgacacg ggggccagcg gcatttctgt
     1561 caccaaaacc accacggtta tcagaaaaac cgccacggtt gccgccaaag ccaccagccc
     1621 cggacggacg gccaccgccg ccaaatttgc ggtcacgcga ctggaagtct gatccacccc
     1681 ggctgtcacg tccaccaaag ccgctgttcg ggcgcgattc aggcatgcgt tgctgcggct
     1741 ccaggcctgg caccacttcg gccttgattg gctggcgggt gaaggcttca atgtcaaaaa
     1801 tcttgcgccg gtcgcggaac tcggcaaaag tcacggccag gccatcacgc ccggcgcggc
     1861 cagtgcggcc gatgcggtgg gtgtagtcct cggccttcat cggcaggcca aagttgaaca
     1921 catgggtgac agtgggcacg tcgaggccac gcgcggccac atcggtggcc accaaaatct
     1981 gcacctgacc ctggcgcagc gccatcaaac ggcggttgcg caggccctgg cttagcgcgc
     2041 catgcaaggc gaccgcatca aaaccatctt gctgcaggtc attggccagg ccgtcgcact
     2101 cgatctgggt gctggcaaac acaatcgcct ggttgatgcc ggtgtcgcgc agccagtggt
     2161 caagcagctt gcgtttgtgc tgggcgttgt cggcccagaa cagcacctgc ttgatgttgg
     2221 cgtgcttttc ctgcgggttg tcgatggtga tgcgttgggg ttcgcgcatg acccgggccg
     2281 ccaactgctg aatgcgcggt gcgaaggtgg cactgaacat catggtctgc ttgcgggtga
     2341 tggtgagctg gttgatttcg gccagatcgt ccgaaaaacc caggtcgagc atgcggtcgg
     2401 cctcatcgac caccagaaac tgcacttggt caagcttgat ctgcatcgaa cgctgcaagt
     2461 ccagcaagcg gcccggcgtg gccaccacca gattggcgtt ctgcaacttg gcaatctgca
     2521 actggtaagg catgccgccg acgatgttgg ctatgcgcag accccggcaa tgttgcacca
     2581 ggtcaatcgc atcatgcgcc acctgctggg ccagttcacg cgtcgggcaa accaccagcg
     2641 caccgggggt tggcgcggtg aagtggcgcg ggttggtcgg gtctttgcgc ttggcgcgct
     2701 tcgggggggc ttcgcctttg gcggcggcat cggctacggc acgctggtag tcctcgcgct
     2761 ctttggtttc ggcatcggct tgctgagtaa gcagggtgtg cagcaccggc agcaaaaatg
     2821 ccgcagtctt gccgctgccg gtctggcttg acaccatcag gtcaataaag cgcgcatcct
     2881 tgaccccgcc gctgtgaccc tgcattgcca gcggaatggt cttttgctgc acgatggtag
     2941 gttggacaaa gcccaggtct ttcacggcgc ggatcagttc gggggccagg cccagggcga
     3001 tgaaaccgtt gggaacttcc ggttcaggcg cggcagcttc agccagcgcc ggtgtgtcga
     3061 tgctgtcgtt tggcagcaca ttgatgtcat tggaaatgat ttcgacagac aaaaagtcgt
     3121 ctgtcgctaa agtagcgtca gtcatggatt tctttcagcg cgaagccccg caggtcgtgc
     3181 gatggccccg ttgatggtta aaaaacatca accatcaaac gaaaccggct ctgactcacc
     3241 gagggtgggt taaaacagcg ctgggggttc tttttttgtc agtcctgcag caggatgacc
     3301 tgaacccggt gaatgttggg agatgtacaa attgcgctgc ggccctgcag tgcagcctgc
     3361 gattatggca cggattcggg tttctaccta agcggtttca agtacgccac cgatggcagg
     3421 tattttcgat gccgctactg agcgccacat tgcggatggc ttggttgttt cgtgtggtgt
     3481 caatcactgt tccttcggtc aacgctgttg catccaatcc ccatcccaat gcaagagcag
     3541 acgcaaccac aaggcatgcc gacagcctcg ggtccttggg tccttgggtt gcagccctgc
     3601 agtgaatctg atcagacagt ctgaggtcat gaagcccaac tttgacgtaa ccggcgctat
     3661 gcaccttgta gcgcggcatc cgtgctgact cccgggttgg gcatcacctt gctagcccgg
     3721 aatctcgggt tcgacaagtg ttgcaagttg cgccagggac tcctgccacc cgaggtagca
     3781 catctcgacg ggaatcatgg cgggtattcc ttcttgcacg atgctgatct cagttccaca
     3841 ggacaccttc gctaatgcca ccgtcacctg catttctccg ggcaagttcg gatcgtcgaa
     3901 tttgtctgtg tagcgaatgg tctggtgggg gacgagttcg aggtactcgc cgccaaacga
     3961 gagcctgttg ccgctggtga aattcgtgaa cgacattcga aatgtgccgc caacccttgc
     4021 atccatctga tgaaccttgc atgtgaaccc gtatggcggt atccatttcg ccatcgcgtc
     4081 ggcatccagg aacgcccgat aaacgcgctc aggaggtgtg cggagcacac ggtgcagttg
     4141 tacagtacct gttgtcatag acaaatttcc tttctttcat caatcgctca gtggctgact
     4201 ggcctgtcaa gcatcgaatg agcactgtct gactgtgaca gccatcttat gcgctcagcc
     4261 gcaaatgaag tccacaggcc gtcgccaaat cggctggagc gagggggtga gcttcgattg
     4321 ggtcggctcg gctactcgaa ccctacacgc ttcaagaacg actcgaccac cggagcccac
     4381 aagtcccgac caacaaagat gagattgtga ccatccgagt tcggaacagg tggcatcgca
     4441 acgaattcgg cgatccctgc gcttgcgaca taagcagcat gccactgtcc gggtgtgtcg
     4501 ggaccccaat atttgtcgtt ttgggcgtaa agccacaagc tcggtacgcg cgcggttttt
     4561 ccatacagga caaaagcctc acgcactttc tcaggggcgc agctcttgcc tggcgattga
     4621 tccgggttgc ctgcgatacc gcccgcgaag ttgatgtagg ccaccacacc aggcggattc
     4681 gctgaagcca aggcggcact cgtcaagcct ccgacggagt tgcctgccac gaggatcctc
     4741 gaactgattg cccacggctg ctcccgcacc caatcgactg ccgccatgat cgcagcagac
     4801 gcagtcgcgg caactcgctc agcgttgggg acaccggagc agtttcccct accatcccag
     4861 ctatgtcctg gaacttcgcg atcattgcca ccggtcttcc cgtacccggg ccgcgcgggc
     4921 gccaccactg cgaaacctct tgcgagccaa aagctcaaga actggcgtgg aattacctcg
     4981 gcaagtgcac ttcgctcctg ctgtgtgccc gacctgccgt gggcatacac caagaccgga
     5041 aatggccctg gaccagcggg cttgaacaat tcagtggaca gatcaatcgt tgaaccaaag
     5101 atgccgcctc ccttcatggg tactttcacg agctgaattg gcggtgcggc gggagccaag
     5161 gtttgcgcac tacagatcgg cattgccgat ccgattagga tcaccgcaca tgcgactgct
     5221 gtgctgaacg ggtgattggc aagcttacgg ggaagttgca ttggtgcctc ctgatgaaac
     5281 ctgaagcaat ggagtccgga aaacctgtgg ctttatctgg acattgcgca taaatcatga
     5341 cacgaaattc tcccgaaagt ggcttgcaac ctagccaata caccgaaaca ccaagaaaaa
     5401 tctcgccatg aatcttctat gaagcccggc gcacgaccaa ggcaatcaac ccgcctgctg
     5461 gaccagttgc gctagcgcag tcgctacctg caccagagcc tgagcactca aaaagttgac
     5521 ccaaaccggg tgcggccttt catcaggtgc cacggttgca cccgagatcg aggcgttcct
     5581 gactatgctg ggcgaccgac cacagggtac gcgagcacat ccgggacatg cacgatagat
     5641 tgagcgccga taaagtttat ccataccggg tacggttttt catccggtgc catgggtgcg
     5701 ccggcgattg acgcgttcgt gaccatgccg ggcaacatcc acaagagacc gccactgtgc
     5761 aagctggtgg ctgcagcagc agcagcgcaa gcgtctgcca ggttgccagc ggcacggact
     5821 caaacgcccg caattgcgag gtatcgcgca tcgggttgac gcaagcgatc aattggtttg
     5881 gtggatcagt ttgaatccgc cggtcagcaa acaaatgtgc gccaatcgtc cgtacaaaat
     5941 gccgctcaca actcaagcct tgagcagcgc atcaatgccc gacaaaccag ccagcgcgcc
     6001 gcaggccagc ccgacatcct ggctgccggg cacctcgggc tcgcgcgggt tgatgcggat
     6061 gatgcgggca ccgtatcgtc ggctaacctg ttgactgaag tgccggaccg agggaatggc
     6121 cgtgccggca cccaattcaa cgataaccgg tgtggccgca tgctggctga gcgaatccag
     6181 ccactggtcc atccgccggt gttgcgcctg gccgcgccgg tgctgataaa gccagtcgcc
     6241 aaacatcaag atattgggcc gtgccaggcc accgcaatgc gggcagacgg gcgggtcatt
     6301 gagcaggcgg caggtgtcgt tgtcaaccac cggggtgaac ccggctgcgc tccacacgtc
     6361 tgactggcaa tcttgcaggc attgcaggtg gtggatcgag ccatggcatt catgaatcag
     6421 ttcctcggca aacccggcct tctgaaactg cccatcgaca ttgctggtga atacccagga
     6481 gcccagccgc atgcgctggc cccattggcg cagcatgtca aagccggcat ggggaacggt
     6541 ttgccggtac agcccgagcc gatggccata aaagccccag gccagaccgg catcgctggc
     6601 aaacatgtct ggatttgcga cttcggtgaa atcaagctgc gcacgcgcca gtgccgggta
     6661 ggcctgccag aaaccttcgc tgccgcgaaa gtccggcagg ccggaatcca cgccgatacc
     6721 ggccccggca ccgatcacca gcgcatcggc accggctatc agttctgcgg cgcgttgcag
     6781 gctggcggca tgacgatcag tgggcagcac ggacatcgtt ctgattcctt tcactgtgcg
     6841 aaacattcgc ctggcacaaa gcccggccct caattcaacc cggcgatacg ctatggcagc
     6901 gacatcattt cacggtcacg acaccggcaa tttcagcagg tgttcccggt agtgctccag
     6961 ttcgtcaatc gactcataca catcggccag cgcggtatgg cgctgcttct ttttgaaacc
     7021 cttgtaaaca tcgggtgccc agcgcttggc caattccttg agcgtgctga catcaaggtt
     7081 gcggtaatga aagaaggctt ccagccgggg catgtacttg gccagaaagc gccggtcctg
     7141 cccgatggtg ttgccacaca tgggcgagcc gcttttgggc acatagtgct tcaaaaaggc
     7201 cagaatctgc tcctgcgcct gctcttcggt cagggttgag gcccgcaccc gatcaatcag
     7261 gccgcttttg ctgtgggtgc tttggttcca cttgtccatg gcggccagca gctcatcact
     7321 ttggtgtacc accagcaccg ggccttcaat gcgtggctcc agtttcgggc cggtgacgat
     7381 gacggcgatt tcaaggatgc gctcggtctc gggctccagg ccggtcattt cgcagtcgag
     7441 ccagaccaga ttctggtcgg atttcgccag gctggcagta ttggtggggg tgttcggtgt
     7501 gctcatggcg gcaaattgtc gctgatcgcc taaacttcgc cgccatgatc gaaaccaagc
     7561 tgtcttctga cccctctgct ttgctgtcaa tgacgtttgc cgtggccctt gccgtcagcg
     7621 tgctgctcaa actctggctg gcatcgcgcc aggttcgcca tgtcgccctg catcgcgacc
     7681 aggtgcctgc cgcgttcgca caaaccattt ccctggcggc tcaccaaaag gccgctgact
     7741 acacgctggc caaggcccgg ctgggctttc tggagctgcc gcttaacctc attgttttgc
     7801 tgggtttcac gctgctgggc ggtttatcgg cgctcaatga cctgttggtg cagtggctgg
     7861 gcacgggttt gctgcagcaa ttggtgctgc tggtcagctt ttccctgatc accgggctga
     7921 tcgatttgcc gctgtcgctg taccagactt ttgtgctgga acaacgcttt ggcttcaaca
     7981 agatgacctt ggggctctgg ctggccgact tgctcaagtc cagcctgatg ggtgccctga
     8041 ttggcttgcc gattgccgcg ctgattctgt ggctgatggc agccagcggt ggcttgtggt
     8101 ggctgtgggc ctggtgcgtc tggatgggct ttaatctgct gctgctggtg atctacccga
     8161 ccttgatcgc gccgattttc aacaaattca cagcgctgga agatgccacc ctgaaggcca
     8221 gggtcacggc cctgatgcaa cgctgcggtt tctcggccaa aggcctgttt gtcatggatg
     8281 gcagcaagcg cagcgcccac gccaacgcct atttcaccgg ctttggcgcg gccaagcggg
     8341 tggtgtttta tgacaccttg ctgtcgaaat tgagccccgc tgaagtggat gcggtgctgg
     8401 cgcacgagtt gggccatttc aagcaccggc acatcatcaa gcgcatcgtg tcgatgtttg
     8461 ccatcagtct ggctggcttt gcactgctgg gctggctggc gcaacaagcc tggttctaca
     8521 ccggcctggg ggtgatgccc aacctgaccg gccccaatga cgcgctggcg ctgctgctgt
     8581 tcatgctggc gatgccggtg tttggcttct tcgttgcccc ggtgatggcg cacttttcac
     8641 gcaaacacga gtttgaagcc gatgcctatg ccatggccca gaccagcggc ccggacctgg
     8701 ccagcgcgct gctcaagctc tacgaagaca atgccgcaac gctgacacct gatccgctct
     8761 atgtggcggt ttattactcg caccccggcg catcggagcg gctggcgaga atgcacccga
     8821 ttgagtcggc cctggcctag cgctgcttcg tgcaggcatc ttgccaggcc tgaagccatc
     8881 ccacacattt acacaaaata tgcctcaagc ccttaccaga cgtgcgctga cagctactga
     8941 agttataaca agcctggcca agttggaagg ctggcgactg tcaggcgacg gggctgctgt
     9001 ggcgattgaa aagtcttttg ccttcgccga cttttaccag accatggcgt ttgtcaacgc
     9061 cctcgccttc atcgcccatg ggcaagacca tcaccccgag ctgatcgtgc aattccgcga
     9121 atgcctggtg cgctggcgta cccacgacct cggtggcctg agcaagtcag actttgactg
     9181 tgccgcacgg gttgaagcgc tgctggcaaa tgcgcgtggc tgaaccatcg gtatcgcaac
     9241 gccagcgcat gcaccgccgg gccgggcagc gccgatgaaa atcaatgcca gggcaaccat
     9301 tgcggccaac acccgccatc agtccggcct gatcgtggcc aactttggcc gacattttct
     9361 ggtggaaaac gccgccggtg agcgcctgat ctgccattca cgcggcaaaa aaagccaggg
     9421 tgtggtgggt gacaaggtgc tgtggcaggc ctccgaggac gagggcacga tcgagaagat
     9481 agaagcccgg cgcaacctgt tttaccgcca ggatgaggtc cgcaccaagt cctttgccgc
     9541 caacctggat caggtgctga tcctgatcgc agccgagccc gagttctcca gcagccaact
     9601 gtcgcgcgcc ttgattgccg ccgaagaaca gcacatcacc ccgatcattg cgctcaacaa
     9661 gagcgacttg accgagccgt tttcccgcgc ctggcactgg ttggaaccct accgccagat
     9721 gggctatgac gtgctggcgc tgtcgctggc ggctggcagc caggccgatc gtgatgcctt
     9781 gacacagcgg ctgcgtggca aaaccacact ggtgcttggg ccctccgggg ctggcaagag
     9841 cacgctgatc aactgcctga ttcccggcgc gctggtgcaa actggcgagc tgtcgcaggc
     9901 gctcagttct ggcaaacaca ccaccaccag caccacctgg tactgggtcg atgccgccaa
     9961 aacgacagcg ttgctggatt cgcccgggtt ccaggagttc ggcctgaacc atatcgaccc
    10021 ggcacaactg gcggcctaca tgcccgatct gaaaccccat gtggcgcagt gcaagttcta
    10081 taactgcagc cacctgcatg agccgggctg cggcgtcatt tcaatggtca aatcaggcgc
    10141 tagcgcagac ggcataagcg ctaaccgcta caaaatttac agtgacttgt atgccgagct
    10201 gaaacagacc cggcgctatt gatggtcggc gcagtgacat ggcgcggtgc aagttcttta
    10261 acagcagcca cctgcatgag cccggctgcg gtgtagtttc aatggtcaaa tcaggcgcta
    10321 gccatgatgg cataagcgct aactgctgca acttttgtag tgacttggat gctgagctga
    10381 aacagacccg gcgctattga tggtcggcgc aatgacatcg cgcagggtaa gtttgataac
    10441 tgcagccacc tgcatgaacc tggccgcagt gtcacttcaa tggtcaaatc aggcgctagc
    10501 ccgcatggaa taagcgccaa tcgctacaaa attgacagta atttggatac tgagttgaaa
    10561 cagacccggc gctatcgatg gttggcacaa cggccagccg cagcagaccg gcagacgacg
    10621 ctggcctgct acggcaggtg ctggccgggg ccgacggcag gtctggttcc attcccgaat
    10681 acccgtgcct cagcacccgg tcgcattcat tgcaaacgcc aggcatggcg agataacagc
    10741 ccagatggtt cagaagcttg atgagatcgc ctgaatcagc agatcgcact gttgcggcgt
    10801 gccaacgctg atgcgcagat attggtcgat gcgtggcaag ctgaaatgcc gcaccagcac
    10861 gccacgcgcg cgcaaatctg ccgccagcgc agccgcatca cgcgaccggt ggcgcgcaaa
    10921 aacaaagttt gcctgtgatg gcagcaccac aaaacccagg tcttccagcg ccagcaccag
    10981 gccctcgcgg ctgctcatca cggcctggcg ggtctgctca aaatacccct ggtcttcaaa
    11041 tgcagccacg gcaccggcaa tcgccagccg atccagcggg taagagttga aactgttttt
    11101 gacccgctgc agcgcctcga tcaggtgtgg ctgggcacag gcatagccaa tacgcaatcc
    11161 ggccagcgag cgcgacttcg acagggtgtg aaccaccagc aggttggggt aacggccaat
    11221 cagttcgagc gcgctttggc cgccaaaatc gacataggct tcatcgacca gcaccacccg
    11281 ctttgggtgc atctgcagca gtgtttcgat gtctgccagc ggcaagccga caccggtggg
    11341 cgcattcggg ttggcaagca ccacgccagc gaccttcaac ccatccgcac aggcatagtc
    11401 agccacattc aggcgcaggt cttcggtcaa gggcacgact tgtgctgcaa tgctgtaaag
    11461 cctgcaataa acttggtaaa agctgtagct gatgtcaggc atcagcagtg gcaagccttg
    11521 ctggaagaag gcaaaaaaag catgggccag cacctcgtcc gagccattgc ccaaaaacac
    11581 atgtttttct gcaatgccgt ggtgcgccgc tatggcctgg cgcagcgcac tggcatccgg
    11641 gtcggggtag agctgcaaac cctgttgtgc ggcacgggtg atggccaaca ccaccctggg
    11701 cgaaggcggg tacgggtttt cgttggtgtt gagcttgacc agcttgtcga ttttgggttg
    11761 ttcacccgga acatagggca ccaggcgtgc aagaaccggg ctgaagaaac tcagggacat
    11821 cacagattca ctctaaattc aatagctgcc tacgcatatg cagcaagggc tggagcacaa
    11881 attcgcttga aaactaacca agcagccggg ccagcgtcaa gagggccagc aggacgatcc
    11941 acatcaccac cgtgcgccat accaaaccca ccacaaccgc caggtgctgc agttccggca
    12001 acag
//
LOCUS       DAOSLL010000058        11929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5372_length_11929_cov_5.028044, whole genome shotgun sequence.
ACCESSION   DAOSLL010000058 DAOSLL010000000
VERSION     DAOSLL010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11929
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5372_length_11929_cov_5.028044"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1459)
                     /locus_tag="PLB25_03985"
     CDS             complement(<1..1459)
                     /locus_tag="PLB25_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40778.1"
                     /translation="MLNYSLALSNPPLLVVSDRLTIRIHTQFTGHPSQTYAVGIEELD
                     QPDKLALLRRIWTAPESFKPKITNRDITEAAAKSFATLAEGLRRRGDGPQARPADKWG
                     SDSGFAQQSADSDPHLSALTDSEALANRVAHFLTQCLFCSFVEDVGLLPGRMFERLVN
                     NRHITPAKLTQGLTDLFHAMANGGMFGADDIPWFNGGLFQRIDVPTLSILDETELHNA
                     ADLNWSAIDVSIFGTLFERGLDPGKRSQLGAHYTDTATIDRIIDPVIRHPLLQKWELV
                     RQAIQGLQAKSKLKRRGAYSAGGRHFKASSGDAAFQQAEQLFADWLDFLTHYRVLDPA
                     CGSGNFLFLALKCLKDIELQSHLQAADLGLDRQTDLVTGPHNLLGIELNEYAAELARV
                     TVWIGELQWRIAHGFELKTNPVLEPLDHIECRDALLCFAAKASDVPPPMNLDAAEAQC
                     GMPGTITLNAAASAQTGAQAGAQAGAQAGAQAGAQA"
     gene            complement(1407..1700)
                     /locus_tag="PLB25_03990"
     CDS             complement(1407..1700)
                     /locus_tag="PLB25_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40779.1"
                     /translation="MAFPARPRRSTKSRARKAIFFLDLCELLGVPKPGSAEDYRFEEK
                     SAVIGGKTGYADVFKRGIFAWENKAPGKKPRHRAETVAQLQPGPEQPAAAGGV"
     gene            complement(2214..3389)
                     /locus_tag="PLB25_03995"
     CDS             complement(2214..3389)
                     /locus_tag="PLB25_03995"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOE40780.1"
                     /translation="MTSAVIVSTARTPLAKSWKGSFNMTHGATLGGHAVEYAIARAGI
                     DAAEVEDVIIGCALPEGATGANIARQIALRADCPVSVPGFTVNRFCSSGLQAIALAAQ
                     RIIAGEGDTYVAGGVESISCVQQELNKHMRTDPWLVKHKPEIYWDMLQTAENVARRYG
                     IGREAMDEYGAASQQRASAALDAGRFTQEIAPIAVTMGIADKVLGLGTKKVTVSQDEG
                     IRAGTTFDGISGLRSAMPGGLITAGNASQFSDGAGAMVLMHEAVAARKGLQPLGRFLG
                     FAVAGCEPDEMGIGPVFAVPKLLKRLGLTVQDIDLWELNEAFAVQVIYCRDKLGIDNA
                     RLNVNGGAIAVGHPYGVSGQRLTGHALIEGKRRGAKRVAVTMCIGGGMGACGVFEVC"
     gene            complement(3590..5686)
                     /locus_tag="PLB25_04000"
     CDS             complement(3590..5686)
                     /locus_tag="PLB25_04000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOE40781.1"
                     /translation="MTAEYAVQGNVAVITLNNPPVNGLGLATRLAITDGLARALADGV
                     VKAIVITGAGKAFSGGADITEFGSPKAFQEPNLPSVILALEASTKPVIAAVHSVCMGG
                     GLELALGCHYRLAAPGCSVALPEVKLGLLPGAGGTQRLPRALGVEAALNLIVSGEPVN
                     SETLATLPGQQLFQQMAASPDTLLTEALAFAAAVADVRPLPMVRSLPCRHPQGDAYFG
                     FARNMVKGMAKNYPAPAKCVDMVEAATRARFEGGLAQEREAFLNLMWTAEHRALKHLF
                     LAERAASKVPDVPVDTAQRAIQSVAIVGAGTMGGGIAMNFLNAGLPVKLLEMKQDALD
                     RGLATIRKNYQAQVNKGKLKPDKCEARLALLSSTLDYDDLKQADLVIEAVFEDMGVKE
                     AVFRQLDRVMKPGAILASNTSTLDLNQIAAFTERPQDVVGLHFFSPANVMKLLEVVRG
                     EQTAKDVLATVMALAKKIKKTAVVSGVCDGFIGNRMIEQYGRQAGFLLDEGCTPAQVD
                     RAVEKFGFAMGPFRMGDLAGNDIGWAIRKRRYLEKPHQKYSKTADLLCEKGRFGQKTG
                     AGWYDYVPGKREPIPNAEVVAMVDAHRASLGITPRKIADEEIVQRLVFSLVNEAAHIL
                     EDGIASKASDIDIVYIFGYGFPVYRGGPMNYANELGLFNVVQAMQRFAQNPLDDAQFW
                     QPAPLLARLAAEGGVF"
     gene            6024..8657
                     /locus_tag="PLB25_04005"
     CDS             6024..8657
                     /locus_tag="PLB25_04005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HOE40782.1"
                     /translation="MESINCPGSNESRQLEIIGQTGCIGYWEYDLPTRSVLLPEASRR
                     LLARITKGPVEAGRSFIDSLSASDRARAQAALDQAAAAQGAFQIELQLICSDGQSAGI
                     SVRGAPVTMDAGCTCLAGTFQGICGERPGEAEHGKAHAQLQALLDVLPQGVSVIDQNL
                     RLILWNRRLHEILGFPQQMVFKNARFEDFIRFNALRGEYGPGDPETQVRDIVARASQF
                     VPHQFERQLKDGRSLRVEGFPFKFAGEISGFVTTYTDITDQRRTEEQLTRQRDVMRTV
                     IDNFPGGISLCDADLRFTAYNDQFRAMLDFPPELFEKGWADFEELIRFNARRGEYGPG
                     DEEEQVRAAVNRARNFEAHRIERLRPNGTWLDIRGTPIPSGGFVTSYIDITDIKQAAE
                     YEQFYRQTLELLVNGDPLPSILLAIVRGIEQIHPAMLCSIVLLDRDGKRLGQVIAPSL
                     PDGFNAAMQGVAIGAGVGSCGTAAFTGARVIVEDIATHPYWEGYRALALQHRLGACWS
                     QPIKSTSGQVMGTFSIYHHQPHTPAPADIALIEQSAQLASIAIERSLVAEKIRDSEAH
                     FRLLTEEVADVVWKADHKLFITYISPADQRLRGYPASEVLGHHVFEMFTDEGIAAVKK
                     IMSQRRQPEPGGRQDGFVTFEAQHRCKDGRLLWGEVQAKPERDAQGVITGYHGITRET
                     TRRKQLEDQVRQLAFFDPLTKLPNRRLLNDRLTQSIAVSQRKDCHCALMVLDLDNFKP
                     LNDAHGHLVGDLLLVEVAERLRGCVREIDTVARFGGDEFVVLLSDLSASRAEATAQAG
                     VVAEKVRHSLCAPYLLLVHHPTQETETVAHRSSASIGVVVYLGREVRPEDILKWADAA
                     MYQAKEAGRNAIRLHDAPARL"
     gene            9370..9885
                     /locus_tag="PLB25_04010"
     CDS             9370..9885
                     /locus_tag="PLB25_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40783.1"
                     /translation="MHCVTTSDNTQLYVRDLVSRHAGKAELPISTTQPLRLKTSDNPM
                     GTDQPAFEATALARKEDRPKFFADDAKPKAADEETLQWALGDSLQARLGCATSFASTD
                     FRSELSAFKVPTRIIDTTEGSIVPVAVSSCLAAKGTWQSGLVEFAGAPRGLFATDKHR
                     LPQQLLNVIGQ"
     gene            9903..10691
                     /locus_tag="PLB25_04015"
     CDS             9903..10691
                     /locus_tag="PLB25_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE40784.1"
                     /translation="MLEQLGQRVIAALTQIYALLGFLGESAMALAGWLAHPARIRWRP
                     VLFNLRGAGFDALPIVGTLSFLLGIVVAYQGADQLRQYGANIFVTDLVGLSMLREFAP
                     LMTAIIIAGRSGSAYAAQIGTMAVTQEIDAMRTIGIAPLEMLVLPKIVALVIALPLLT
                     VFADLLGVAGGMLMARAQLGIGFGEFLDRFSKAVSVTSYLVGIGKAPVFAAIIVLVGC
                     FQGFRTRGGADSVGQQTTRAVVQSIFLVIVADALFSIAFSVLDL"
     gene            10693..11520
                     /locus_tag="PLB25_04020"
     CDS             10693..11520
                     /locus_tag="PLB25_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006462994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE40785.1"
                     /translation="MPNQPNQPAGPETVLEMHQVVTRFGTHLVHSGVDLEVRRSEIMA
                     VIGGSGSGKSTLLREMILLQMPDEGHIKVLGVDLANITDAAALALRQRFGVLFQHGGL
                     FGALTVLENIGLPLREHTQKNNQDIDAIAAKKLAEVGLEEAVASQYPSELSGGMLKRA
                     ALARALVLGPELLFLDEPTAGLDPQSAAGIDTLVLALRRQSGLTIVMITHDLDLLWQV
                     TDRVAVLADGKVQAVGTMAELAKMDNPTIQPFFDGPRARSAQAQATSQSKEPSSKPK"
     gene            11517..>11929
                     /locus_tag="PLB25_04025"
     CDS             11517..>11929
                     /locus_tag="PLB25_04025"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOE40786.1"
                     /translation="MNYALVGAFVLALGALLVAGALWLASGGAWQTKYDMYLAVEEES
                     VAGLNINAPVKYNGVDVGKVQTIALDQINPKTVNLFFAIERGTPVKEDTIAILKTQGL
                     TGIAYVELSGGSVSSPLLRAKEGAGYPVIQTAPSLS"
BASE COUNT         2270 a         3578 c         3673 g         2408 t
ORIGIN      
        1 cggcctgcgc cccggcctgc gccccggcct gcgccccggc ctgcgccccg gcctgcgccc
       61 cggtctgcgc gctggcagcg gcgttcaaag tgatggtgcc aggcatcccg cactgggcct
      121 ctgcggcatc caggttcatg ggcggcggca cgtcgctcgc ctttgccgca aagcacagca
      181 gcgcatcgcg gcattcaatg tgatccagcg gctccagcac cgggttggtt ttcagctcaa
      241 acccatgggc aatgcgccat tgcagttcac caatccagac cgtcacccgc gccagttcgg
      301 cggcgtattc gttgagttcg atgcccagca ggttgtgcgg gccggtcacc agatcggtct
      361 ggcggtcgag ccccaggtcg gcggcttgca gatggctttg cagctcgatg tctttgaggc
      421 acttgagcgc cagaaacaaa aaattgccac tgccacaggc cggatccagc acgcggtaat
      481 gagtcagaaa gtccagccag tcggcaaaca gttgctccgc ttgctgaaac gcggcatctc
      541 ccgaagatgc tttgaagtgc cgcccaccgg cagaataggc tccccggcgt tttagcttgc
      601 ttttggcctg cagcccttgt atggcctgcc taaccagctc ccatttttgt agtaacgggt
      661 ggcgtatcac ggggtcaatg atgcggtcga tggtggcagt gtcggtgtag tgggcaccga
      721 gctggctgcg tttacccggg tccagaccgc gctcaaacag cgtgccaaag atggaaacgt
      781 cgatggcgct ccagttcagg tcggcggcgt tgtgcagttc ggtctcgtcc agaatcgaca
      841 gggtcggcac gtcgatgcgc tggaacaggc cgccattgaa ccaggggatg tcgtccgcgc
      901 caaacatgcc gccattggcc atggcgtgga acaagtcggt caggccttgt gtcagcttgg
      961 ccggggtaat gtggcggttg ttcaccagtc gctcgaacat gcgaccgggc aacagaccaa
     1021 cgtcttcgac aaaggagcag aacaggcact gggtcagaaa gtgggcgaca cggttggcta
     1081 acgcttcaga gtcggtaagt gccgataaat gggggtcaga gtccgcgctt tgctgcgcaa
     1141 agccggaatc cgacccccat ttgtctgccg ggcgcgcctg tgggccatcg ccacgtcgac
     1201 gcaggccttc cgccagggtg gcaaagcttt tggctgccgc ttcggtgatg tcgcggttgg
     1261 tgatttttgg cttgaagctt tcgggggcgg tccagatgcg gcgcagcaaa gccaacttgt
     1321 cgggctggtc aagctcttcg atgccgaccg cgtaggtctg gctcgggtgg ccggtgaact
     1381 gggtgtggat gcgaatcgtc aggcggtcag acaccaccag cagcggcggg ttgctcaggg
     1441 ccaggctgta gttgagcaac tgtttcagcg cggtgtcgag gttttttgcc gggggccttg
     1501 ttttcccacg cgaagatgcc gcgcttgaag acatcggcat agccggtctt gccgccaatc
     1561 accgcgcttt tttcttcaaa ccggtaatcc tcggcactgc ccggcttggg cacaccgagc
     1621 agttcgcaca ggtccagaaa gaaaatggct ttgcgcgccc tgctcttcgt tgagcgccta
     1681 ggccgggccg ggaatgccac cgggtgcgcc ccatttggcg ataaaggctt gcgatgtcat
     1741 gggtcgagtc atcagcagac tatgcggggg actcaaaatg ccgcttcagc catccccgca
     1801 ccagtgtcgt cacggctggc caccacctgc agtcagacca cttttttggt cggttcaaat
     1861 tgaatcaacc taagccggac aagccagaat cgaacagcct tcgccgcgag ggcgcgactc
     1921 ctgcacaaac agccgttgcc catttgaatt tgtaggagtc gcgccctcgc gacgaatgac
     1981 ttcatgactt tcttgaattc ttctcggaaa ttgcaaagac ttgacttgtt agagtcgagc
     2041 accgcgtcaa agcttgcccg gctgtgcttg tgcaaatcgt gcgcgagtga tcaggcgcgc
     2101 agcgcccagc cagtcgtgca gcagacaatc caggatgttg caaaagctca tcaactgatg
     2161 aataaaaagt gcctccagac cgcttggaat atgcctgagc agctacaaaa acattagcaa
     2221 acttcaaaca ccccgcaagc ccccatgccg ccgccgatgc acatggtcac agccacgcgt
     2281 ttggcacccc ggcgtttgcc ttcgatcagg gcatggccgg tcagtcgctg gccgctgaca
     2341 ccgtaggggt gaccaaccgc aatcgcgccg ccgttgacgt tgagccgggc gttgtcgatg
     2401 ccgagcttgt cgcggcaata gatcacctgc acggcaaagg cttcgttgag ttcccacagg
     2461 tcaatgtctt gcaccgtcag gcccaggcgc ttgagcagct tgggtacggc aaacaccggg
     2521 ccaatgccca tctcgtcagg ctcgcagccg gccacagcaa agcccagaaa gcggcccagc
     2581 ggctgcaggc ccttgcgcgc cgccaccgct tcatgcatca gcaccatggc accggcaccg
     2641 tccgagaact ggctggcatt gccagccgtg atcaggccac ccggcatggc cgagcgcagc
     2701 ccgctgatgc cgtcaaatgt ggtgccagcg cgaatgcctt cgtcctggct gacggtgact
     2761 tttttggtgc ccaggcccag taccttgtcg gcaatgccca tggtcacggc aatcggcgcg
     2821 atttcttgcg tgaagcgacc agcgtcgagc gcggcgctgg cgcgttgctg gctggcggcg
     2881 ccgtattcgt ccatcgcctc gcgcccgatg ccgtagcgtc tggccacgtt ttcggcggtt
     2941 tgcagcatgt cccagtaaat ctcgggtttg tgcttgacca gccaggggtc ggtgcgcatg
     3001 tgcttgttga gttcctgctg cacgcacgaa atgctctcga caccgccggc cacataggtg
     3061 tcgccctcac cggcgatgat gcgctgcgcc gccagtgcaa tggcttgcag gccggacgag
     3121 caaaagcggt tcacggtgaa gccgggcacg ctgaccggac agtcggcccg cagcgcgatc
     3181 tggcgggcaa tgttggcacc ggtggcacct tcgggcaggg cgcagccgat gatcacgtct
     3241 tccacttctg cggcatcaat gccagcgcgg gcgatggcgt attcgaccgc atgaccacca
     3301 agcgtggcac cgtgcgtcat gttgaaggaa cccttccagc ttttggccag cggggtgcgg
     3361 gcggtggaga cgattacggc ggatgtcatg gtgtactttc tgatgtgtca aatttgatag
     3421 cttgcagcgc ttgctggacg ggctctggag gctgatttat cgtgtggttc cgcagaattg
     3481 gggtcagagc ccaattctgc gatgcgattg tggtgaattg ccaggtctcg ggccgaattg
     3541 ggatccgacc ccaaatctgc tacgccgcgc gtgcagttgg caagcgtcat caaaagaccc
     3601 cgccctcggc agccagcctg gccagcagcg gtgcaggttg ccagaattgg gcatcgtcta
     3661 gcggattttg ggcaaagcgc tgcattgcct gcaccacgtt gaacaggccc agctcgttgg
     3721 cgtagttcat cgggccgccc cggtacaccg gaaagccgta gccaaagatg tagacgatgt
     3781 cgatgtcgct ggccttgctg gcaatgccgt cttccagaat gtgcgcggcc tcgttgacca
     3841 gcgaaaacac caggcgctgc acgatttctt cgtcagcaat cttgcgcggt gtgatgccca
     3901 gactggcacg gtgcgcatcg accattgcca ccacctcggc attcggtatt ggctcgcgct
     3961 tgcccggcac atagtcatac cagccagcac cggttttctg gccaaagcgg cctttttcgc
     4021 acaacaggtc ggcggtcttg ctgtatttct ggtgaggctt ttccagatag cggcgcttgc
     4081 ggatggccca gccgatgtcg ttaccagcca ggtcgcccat gcgaaacggc cccatggcaa
     4141 agccaaactt ttccaccgcc cggtccactt gcgcgggggt gcagccttcg tcgagcaaaa
     4201 agccggcctg gcgaccatat tgctcgatca tgcggttgcc gataaagccg tcacacacgc
     4261 ccgagaccac cgccgtcttt ttgatctttt tggcaagcgc catgaccgtg gccagcacgt
     4321 ctttggcggt ttgttcaccg cgcaccactt ccagcagctt catcacattg gccgggctga
     4381 aaaagtgcag ccccaccacg tcttgcggcc gttcggtgaa cgcggcaatc tggttcaaat
     4441 ccagcgtcga ggtgttggag gccagaatcg cgcccggctt catcacccgg tcgagctgcc
     4501 ggaacacggc ttctttcacg cccatgtctt caaacaccgc ttcgatcacc aggtcggcct
     4561 gtttgaggtc gtcatagtcc agcgtgctgc ttagcaaggc caggcgcgcc tcgcatttgt
     4621 ccggcttgag tttgcctttg ttgacctggg cctggtagtt tttgcggatg gtggccagac
     4681 cacggtccag cgcatcttgc ttcatttcca gcagcttcac cggcagaccg gcgttcagga
     4741 aattcatggc aatgccgccg cccatcgtgc cagcgcccac aattgctact gattgaatag
     4801 ctctctgcgc tgtatccacg ggcacgtcag gcacttttga tgctgcacgt tcggccaaaa
     4861 acaggtgttt cagtgcgcgg tgttcagccg tccacatcag gttcagaaag gcttcgcgct
     4921 cttgcgccaa gccgccttca aacctggcgc gggtggcggc ttcgaccatg tcaacgcatt
     4981 tggcaggtgc cgggtaattt ttcgccatgc ccttgaccat gttgcgggca aagccaaagt
     5041 aggcatcgcc ctgcgggtgg cggcacggca ggctgcgcac catcggcagc gggcgcacgt
     5101 ctgccactgc agcggcaaaa gccagcgcct cggtcagcag ggtgtctggc gaagcggcca
     5161 tttgctggaa cagttgctgc cctggcagcg tcgccagcgt ctcgctgttg accggctcgc
     5221 cactgacaat caggttcagc gcagcttcaa cgccaagcgc tcgcggcagg cgctgcgtgc
     5281 caccggcacc cggcaacagt cccaatttga cttcgggcag cgccacactg cagcccggtg
     5341 cagctagccg gtagtggcag cccagtgcca gttccagccc tccacccatg cagaccgagt
     5401 gcaccgccgc aatcaccggt ttggtcgagg cctccagcgc cagaatcacg ctgggcagat
     5461 tgggttcctg aaaggccttg ggcgagccaa attcggtgat gtcggccccg cccgaaaacg
     5521 ccttgcccgc gccggtgatg acgatggcct tgaccacccc gtctgccagc gcacgcgcca
     5581 gcccgtcggt gatggccagc cgggtggcca gacccaggcc attgacaggc gggttgttga
     5641 gcgtgatcac ggcaacattg ccctgcacgg cgtattcagc ggtcatgaga cttctccaaa
     5701 aaaagaacga gcgttcgtta attgtaggaa gacatcagcc atgcgcctgg ccgactggcc
     5761 cattgacagt cgcctgcggg acaagccatt tgccgggcaa acggcgcaaa agcccgccgc
     5821 tggtgtccag ccaagcaact tccagccctg tccaggcatg gcagtcattg tcaggcagca
     5881 gcgcccgctg ccgtcatcgg tcagagaacc ggcgcggcaa ttcgcagcgg gcaaacacag
     5941 gggtttgcgc tggcccgctg gtgcgatact tggaccgcaa gcgcttgatt ttcctgtccg
     6001 ttcacccgac ccgaggcaaa tgcatggaat cgattaactg ccccggcagc aacgaaagcc
     6061 gacaactcga aatcatcggg cagaccggct gcattggcta ttgggaatac gatttgccaa
     6121 cccgttcggt gctgctgcct gaggcttcac gcaggctgtt ggcacgcatc acgaaaggcc
     6181 cggtcgaggc gggccggtca ttcatcgaca gcctgtcggc atcggatcgt gcgcgtgctc
     6241 aagcggcgct cgaccaggcg gcggccgctc agggcgcttt tcagatcgaa ttgcaactga
     6301 tctgtagcga cggccagtcg gccggtatct cagttcgcgg tgcgccggtg acgatggacg
     6361 ctggttgtac ctgcctggcg ggcacgttcc agggcatctg cggcgaaaga ccaggcgagg
     6421 cagagcatgg caaggcccat gcgcagttgc aggcactgct tgatgtgctg ccgcaagggg
     6481 tcagcgtgat tgaccagaat cttcggctga tcctgtggaa ccggcgattg catgaaattc
     6541 ttggttttcc gcaacagatg gtgttcaaaa atgccaggtt cgaggatttc atccggttca
     6601 acgcgctgcg cggcgaatac ggccccggtg acccggaaac acaggtgcgc gacattgtgg
     6661 cgcgtgccag ccagtttgtg ccacaccagt tcgaacgcca gctcaaagat ggccgcagcc
     6721 tgcgggtgga gggttttccg ttcaagtttg ccggcgaaat atcgggcttt gtcaccacct
     6781 ataccgacat caccgaccag cggcgcaccg aagagcaact gacgcgccag cgcgatgtga
     6841 tgcgcaccgt catcgacaac tttcccggtg gcatctcgct gtgcgatgcc gatctgcgct
     6901 ttaccgccta taacgaccag ttccgggcga tgctggactt tccacccgag ttgttcgaga
     6961 aaggctgggc tgatttcgaa gaactgattc gcttcaacgc ccgccggggc gagtacggtc
     7021 caggggatga ggaagagcag gtgcgggcag ccgtcaaccg tgcccgcaac tttgaggcac
     7081 accggatcga gcgcttgcgg cccaatggca cctggctgga cattcgcggc accccgattc
     7141 ccagcggcgg ctttgtcacc agttacatcg acatcaccga catcaagcag gccgccgagt
     7201 acgagcagtt ttaccggcaa acgctggagt tgctggtcaa tggtgacccg ctgccgagca
     7261 ttctgctggc gattgtgcgt ggcattgagc aaattcatcc tgccatgctg tgcagcatcg
     7321 tgttgcttga ccgcgatgga aagcggctcg gtcaggtgat cgcgccaagc ctgcccgatg
     7381 ggtttaacgc cgccatgcaa ggcgttgcca ttggcgcggg ggtgggttcg tgcggcaccg
     7441 ccgcgttcac cggtgcgcgc gtcatcgtcg aagacatcgc cacccatccg tattgggagg
     7501 ggtaccgcgc gctggctttg cagcaccgtc tgggggcctg ctggtcgcaa ccgatcaagt
     7561 ccaccagcgg ccaggtgatg ggcacgtttt caatttacca ccatcagccg catacgcctg
     7621 cccccgctga cattgccctg atcgagcagt cggcgcaact ggccagcatt gccattgagc
     7681 gcagtctggt ggcggaaaaa attcgcgaca gcgaggctca ctttcggttg ctgaccgaag
     7741 aggtggcaga cgtggtctgg aaggccgacc acaagctgtt catcacttac atcagcccgg
     7801 ccgaccagcg cttgcgcggc tacccggcca gcgaggtgct gggtcaccat gttttcgaga
     7861 tgttcaccga tgaaggcatt gcagcagtta aaaaaatcat gtcccagcgt cggcagcctg
     7921 aaccaggggg ccggcaggat ggctttgtca cgttcgaggc gcaacatcgc tgcaaggacg
     7981 ggcgcttgct gtggggcgag gtgcaagcca aaccggagcg cgacgcgcag ggtgtcatca
     8041 ccggctacca cggcattacc cgggaaacca cccggcgcaa gcagttggaa gaccaggtgc
     8101 gacaactggc gttttttgac ccgctgacca aactgccaaa ccgtcgtctg ctcaacgacc
     8161 ggttgaccca gtcgattgct gtcagccagc gcaaagactg ccattgcgcg ctgatggtgc
     8221 tggacctgga caatttcaaa ccgctcaatg acgcgcatgg gcatctggtt ggcgacttgc
     8281 tgctggtcga agttgccgaa cgtttgcgcg gctgtgtgcg cgagatcgac accgtggcgc
     8341 gatttggcgg tgatgagttt gtggtgttgc tcagtgacct gagcgccagc cgggcagagg
     8401 ccacggcgca ggctggagtc gtcgccgaga aagttcgcca cagcctttgt gcgccttatc
     8461 tgctgctggt gcatcacccg acgcaggaaa cggaaacggt ggcacatcgt tcgtctgcca
     8521 gcattggcgt ggtggtctat ctgggccggg aagtcagacc ggaagacatt ttgaaatggg
     8581 ccgatgcggc gatgtaccag gccaaggagg ccggtcgcaa cgcgatccgg ttgcatgacg
     8641 caccggcccg gttgtgacgc agggctgacc tgctatttgc atcgcccggc gctcagactg
     8701 atcgggcgag tggccggcat ccggggtccg tatttcaaga aaaaaagcac tccagccctt
     8761 gtacagtatg cgctggtagc ttcaataatg atagcttttg tcgagtcagg cgctactgca
     8821 aattgatttc tctggcggct gaattttcaa actggatgcc tcagttgggc gcgcccgagc
     8881 gggccgctgg cggcgcggct gggtattgag gccgtgattg aattgaatga ccccgctgcg
     8941 ctgtgggtgg cacttcgaca ccttgccaaa gcgcatggca tgcagcggtt gcccggcgtg
     9001 ccgatgtggg cgacaagacg cctttcgaaa ccctgaacgc caacggtaac ccaccacacc
     9061 ccagcctgac cttgcctgat gacgtgttgc cagtgctggg gccgggtgca aatgtgacag
     9121 gggttttacc tgccagatgc aactggtgaa tcgccaacgc atgatgattg cgggtctctt
     9181 ggccgacacg gatctggcag atgcctcacg catggccaaa tccttcttgc gacagcaaat
     9241 tgccaccaac gaccgctggg tggcgctggc aacatgaaag atcggtgcgc cgcggtgcct
     9301 tggtacggta tcgcacagac ccgggcctag agcaggacag atgatttgcg ccacttcaag
     9361 ggcaaccgta tgcactgcgt taccacctca gacaacaccc aactctacgt cagggacttg
     9421 gtgtcgcgcc acgctggcaa ggccgagttg ccgatttcga caacccagcc gttgcggctg
     9481 aaaaccagcg acaacccgat gggcacggat cagcctgcct ttgaggccac agcgttggca
     9541 cgcaaagaag accggcccaa gttctttgct gacgatgcca aaccaaaagc agccgacgaa
     9601 gagacactgc aatgggcact tggtgattcg ctgcaggcgc gtctgggctg cgccacatca
     9661 ttcgcatcga ccgactttcg atccgagctg tctgcgttca aggtgccaac acgcatcatt
     9721 gacacgacag aggggagcat cgtccccgta gcggtctcaa gttgccttgc tgccaagggc
     9781 acttggcagt ccgggctggt cgagtttgct ggcgcacctc gcggtttgtt cgccaccgac
     9841 aaacatcggc tgccccagca actgctgaac gtcatcggcc aataaccaat caagcccaat
     9901 tgatgctgga acaattgggt cagcgagtga tcgccgcatt gacgcagatc tatgcgctgt
     9961 tgggcttttt gggtgagagt gccatggcac ttgccggatg gttggcgcat cctgcgcgca
    10021 tccgctggcg gccggttttg ttcaacctgc gaggcgctgg cttcgatgcg ctgcccattg
    10081 tcggcacact gtcgtttctg ctcggcattg tggtggctta tcagggcgca gaccagttgc
    10141 gccagtacgg tgccaacatc ttcgtcactg atttggtggg cttgtcgatg ctgcgtgagt
    10201 ttgcgccgct gatgacggcc atcatcattg ccgggcggtc cgggtcggcc tacgccgcgc
    10261 agattggcac catggcggtg acccaggaaa ttgatgcgat gcgcaccatc ggcatcgcgc
    10321 cactggagat gctggtgctg ccgaaaatcg tggcgctggt gatcgccctg ccgctgctga
    10381 cggtgtttgc cgacctgctc ggagtggccg gcggcatgct gatggcccgc gcccagttgg
    10441 ggattggttt tggcgagttt ctggaccgct tttccaaggc tgtcagcgtc acgtcctatc
    10501 tggtgggcat tggcaaagcc ccggtgtttg ccgccatcat tgtgctggtg ggttgttttc
    10561 agggctttcg cacccgaggg ggcgctgaca gtgttgggca acaaaccacg cgcgcggtgg
    10621 tgcagtcaat attcctggtg atcgtcgccg acgcgctgtt ttccatcgct ttcagtgtgc
    10681 tggacctttg acatgcccaa ccagcccaac cagcccgccg gccctgaaac cgtgctggag
    10741 atgcaccagg tggtgacacg ctttggcacc catctggtac acagcggggt cgatctggaa
    10801 gtgcgacggt ccgagatcat ggccgtgatt ggtggcagcg gctcaggcaa atcaacattg
    10861 ctgcgcgaaa tgattctgtt gcaaatgccc gatgaaggcc atatcaaggt gctgggagtt
    10921 gacctggcca acatcacgga tgcagccgcc ctggccttgc gccaacgctt tggtgtgctg
    10981 tttcagcacg gtggcctgtt tggcgcactg acggtactcg agaacattgg cttgccactg
    11041 cgggaacaca cccaaaaaaa caaccaggac atagatgcca tcgctgccaa gaaactggca
    11101 gaggtcgggc ttgaagaagc ggtggccagt caatacccgt cggagcttag cggcggcatg
    11161 ttgaaacgcg ccgcgctggc ccgggcgctg gtgttggggc ctgagttgct gtttctggac
    11221 gagcccaccg ccgggctgga cccgcaaagc gccgctggca ttgatacgct ggtgctggca
    11281 ttgcggcggc aatcgggcct gaccattgtc atgattaccc acgacctgga tttgctctgg
    11341 caagtgactg atcgggtagc cgtgctggct gacgggaaag tccaggcggt tggcaccatg
    11401 gcggaactcg caaagatgga caaccccacc atccagcctt tttttgacgg cccgcgggcg
    11461 cgctcagccc aggcgcaagc cacatcccaa tccaaggagc catcatcgaa accaaagtga
    11521 actacgccct ggtgggtgct tttgtgcttg ccctgggtgc cctgctggtg gcgggcgcat
    11581 tgtggctggc ctctggtggt gcctggcaaa cgaaatacga catgtatctg gctgtggaag
    11641 aagagtcagt ggcaggcctc aacatcaacg cgccagtcaa gtacaacggg gtcgatgtcg
    11701 gcaaggtgca gaccatcgcg cttgatcaga tcaaccccaa aaccgtgaat ttgttttttg
    11761 ccatagagcg tggcacgccg gtcaaggaag acaccatcgc catcctgaag acccagggcc
    11821 tgaccggcat cgcctatgtg gaactgagcg gcggcagcgt cagctcgccg ctgttgcgcg
    11881 ccaaagaggg tgccggttat ccggtgattc aaaccgcacc ttcgttgtc
//
LOCUS       DAOSLL010000059        11917 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5376_length_11917_cov_4.133704, whole genome shotgun sequence.
ACCESSION   DAOSLL010000059 DAOSLL010000000
VERSION     DAOSLL010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11917)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11917)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11917
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5376_length_11917_cov_4.133704"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(696..887)
                     /locus_tag="PLB25_04030"
     CDS             complement(696..887)
                     /locus_tag="PLB25_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative motility protein"
                     /protein_id="HOE40787.1"
                     /translation="MDIPLTALVAASRQMEQSQVTETARVLVLEKTMDLQASGALALL
                     QALPLATSGNLGTQVNAMA"
     gene            complement(1279..1355)
                     /locus_tag="PLB25_04035"
     tRNA            complement(1279..1355)
                     /locus_tag="PLB25_04035"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1319..1321),aa:Met,seq:cat)
     gene            complement(1402..2181)
                     /locus_tag="PLB25_04040"
     CDS             complement(1402..2181)
                     /locus_tag="PLB25_04040"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HOE40788.1"
                     /translation="MTYELITTRTEADKVGIVTLNRPKQLNALNDQLMTELGAALQAF
                     DADPSIACMIITGSEKAFAAGADIGGMATYSFADVYKGDFITRNWEQIRSVRKPVIAA
                     VSGFALGGGCELAMMCDFIIAADNARFGQPEIKLGIIPGAGGTQRLPRAVGKSKAMDL
                     ALTGRMMDANEAERAGLVSRVVPLDKLMEEALGAALMISEYSQIATMAVKESVNRAFE
                     GGLADGIMFERRLFHALFATADQKEGMHAFVNKRKAVFTHK"
     gene            complement(2171..4021)
                     /locus_tag="PLB25_04045"
     CDS             complement(2171..4021)
                     /locus_tag="PLB25_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M61"
                     /protein_id="HOE40789.1"
                     /translation="MSAVDYRIGMHNLHAHLFGITLTINQPAAMQVVSLPVWIAGSYL
                     VREFSKNLQCLQARQAGSDVPLTQRDKCTWQVHCVAGQPLVLGYQVYAFDNSVRTAWL
                     DTRRGFFNGTSVCLRVHGQEQEEHCLTLESEGVPSDWQVATSLTPQQSGPDGFGTYLA
                     ANYDELVDSPVEMGRFWCGSFTAFGILHRFVVAGAAPTFDGARLLADTRTICEASVNF
                     WHGETTPGTDRAAKMMPPHRHYLFMLNAVDNGYGGLEHRNSTALICQRADLPRLGQTK
                     PLAVADGYVTLLGLISHEYFHTWNVKRLRPAEFVRYDYNQENYTELLWFFEGFTSYFD
                     DLLLRRAGLIDDGTYLSLLNKTINQVLQTPGRHLQTVAQASFDAWVKYYRQDENTANA
                     TISYYTKGSLVALCLDLSLRQGGKTSLDAVMRRLWQLCRGGPMTQDDLLAVLVELSGQ
                     SFEAQIAAWVHGTGDLPLKPLLTRQGVVYREEAAAIGDQLGLRVAEQSGIRIKSVLRG
                     SAAELAGFAAGDEWLGIEVGQDKSAQGWRLAKLDDLPLYLGQSRTFKAWIARDQRLQT
                     LEVTLDDTVANVKLSIDDAKLVGLWLGNTEPAEPAQSAFDDFHKEYHHDL"
     gene            complement(4033..4746)
                     /locus_tag="PLB25_04050"
     CDS             complement(4033..4746)
                     /locus_tag="PLB25_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HOE40790.1"
                     /translation="MKHFNTTVLACGLLAGTGVWAQEASIRKNLAERVPQLQKIDEVS
                     KTPINGLYEVRVNDSEIYYTDAQANYLVQGNLIDTRQKRNLTEERVDKLTAVAFDALP
                     VKDAFTIVRGNGKRKLAVFEDPNCGYCKRFEESLQKVNNITIHMFLYPVLGPGSVEKS
                     KNIWCAKDKGKTWQDWMLDDVEPASAKCDTAAVDRNMQFGKKHKISGTPTLIFADGSR
                     VPGAVGADQIEKRLSQPGG"
     gene            complement(4768..5892)
                     /locus_tag="PLB25_04055"
     CDS             complement(4768..5892)
                     /locus_tag="PLB25_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HOE40791.1"
                     /translation="MTHQFDICIRGAGIVAYTLALLLARDRLRVGLVVQSPAAEPLKP
                     ASTDVRAYALNAKSKALLESVRCWPDELHATAVTSMRVLGDEGGEVNFDAVDLAVPAL
                     TWIVDVPTLEARLSDAVRFQPHIELLDSPQPAALTVVCEGKASRTREEFGVEFAVARY
                     PQRAIAARLTCEKPHAQVARQWFTPGEILAFLPLDGEQGSAVALVWSVLESRAQDLLD
                     ADEEVFCAELAQISKDCQGKLSLCSPRVAWPLQSAQASRWIGVANGQAWALAGDAAHS
                     VHPLAGQGLNLGLGDVAELVSILHKREYWRPISDTRLLRRYERARRSDVSATALAMDG
                     LQQLFSKDGSTWQALRNWGMNGFEHSGLAKDWAARYAMGL"
     gene            6010..7098
                     /gene="ychF"
                     /locus_tag="PLB25_04060"
     CDS             6010..7098
                     /gene="ychF"
                     /locus_tag="PLB25_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HOE40792.1"
                     /translation="MKCGIVGLPNVGKSTLFNALTKAGIAAENYPFCTIEPNVGIVEV
                     PDVRLDALAAIVKPQKIQRAIVEFVDIAGLVAGASTGEGLGNKFLAHIRETDATVNVV
                     RCFEDDNVVHVSGKVDPVSDIEVIQTELCLADLSTVDKALTRYQKTAKSGGDKEAAKL
                     VTILERCQTALNQAKPVRALDFSKEELPLLKQFFLITAKPAMFVANVAEDGFQNNPFL
                     DRLKAYADAQNAPVVAICAKMEAEMADMGEEDKKMFLAEIGQTEPGLNRLIIAAYKLL
                     GLQTYFTAGVKEVRAWTIHVGDTGPQAAGVIHTDFEKGYIRAQTIAFDDFINFKGEQG
                     AKDAGKMRSEGKEYIVKDGDVMNFLFSP"
     gene            complement(7196..7666)
                     /locus_tag="PLB25_04065"
     CDS             complement(7196..7666)
                     /locus_tag="PLB25_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HOE40793.1"
                     /translation="MEPWLEQFLTLLALPKFGLSTVFVVSFISATLLPLGSEPAVFGL
                     VQLNPHLFWPTIGIATLGNTLGGAVSWWMGLASHKVVDRYRHSSHHLRALAWLKKLGP
                     KACLLAWLPLVGDPLCAVAGWLKLPFWPCLAYMAIGKFARYLSMTAALVGMFGG"
     gene            complement(7779..8996)
                     /locus_tag="PLB25_04070"
     CDS             complement(7779..8996)
                     /locus_tag="PLB25_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOE40794.1"
                     /translation="MSAMQNVKVGTLLSAVDSVRNWRAVALMFAALLAAGLFWGLGGL
                     LSVKVHMAVGFLFFLVGLATFFYGVNAVGIMLMDEARGFASRNVLAAVLESLATGHRL
                     ILAFLLFGLIYVLGLLAIALLLLICKVPFIGPFFFTFVFPLSVVLIGVAVFALQMVVL
                     PLTAPAIWAGATTMQAVSRVGAIARARVVNVMLMMIVLTLIVLAVTFTVGSILVVGTM
                     VTAGMAAGILNIGVGFGGMEDLLSSGFEGNGYFVAASVGGGIVYAVGFSLASLVFSRG
                     CCSVYLANLEGVDVAGMEASIQDKVNEARQRAQEARAKAAAAQTQAQAQAQAQAQAQA
                     QAQAQAQAQAQAQAQAQAQAQAQAQARADAPQVAIAPIAVAPVASLNCPRCHAPHLAG
                     DMFCGECGFRLTV"
     gene            complement(9067..9810)
                     /locus_tag="PLB25_04075"
     CDS             complement(9067..9810)
                     /locus_tag="PLB25_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40795.1"
                     /translation="MTAPHKGVMPEIDLSQFDEPPKAAVPRNALPVEPPVKKASSNVI
                     WLAAGALFFALAIAAGGWFWSQRAASTDSLNASAAPSSAALSAGVQPAPVAVEPASSA
                     AAPTVAPPAIITETAPVPAPMPAATPEPAAVPNNNAANQMPSDQPVKPPKPTAKPPVV
                     KKQPMAEPEPRSTSPEPYVAPAQQVPPVATEPIRSASPRDACGKRSFLALAFCMQNEC
                     NSGRFVNHPQCVEMRMQQQEAAQRRDGGR"
     gene            10447..11499
                     /gene="dusB"
                     /locus_tag="PLB25_04080"
     CDS             10447..11499
                     /gene="dusB"
                     /locus_tag="PLB25_04080"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HOE40796.1"
                     /translation="MNLGNHRLLNPVFVAPMAGVTDRPFRQLCKRLGAAYAVSEMLSS
                     RPDLQHSPKTTRRANHAGEPGLIAVQIAGTDPAMMADAARLNIDRGAQVIDINMGCPA
                     KKVCNKWAGSALMQDERLALQIVDAVVQACAPHGVPVTLKMRTGWSQANKNAVQIARL
                     AELAGVQMFTIHGRSREQGYLGQAEYDTLAAVKAMVRVPVVANGDIDSPQKARAVLAA
                     TGADAVMIGRAAQGRPWIFREIVHFIATGETLAQPLVAEVKQLLLAHLQDHYTLYGEH
                     VGLRTARKHIGWYVKTLPGGEAFRAHMNALEDCRLQHDAVADFFDELNLHMDRLPTPT
                     VTDVTHNKQNQPETLT"
     gene            11496..11732
                     /locus_tag="PLB25_04085"
     CDS             11496..11732
                     /locus_tag="PLB25_04085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE40797.1"
                     /translation="MKPPHVADCVRTSLEGYLSDLGSSEPNRMYEMLMSTVEKPLLEV
                     VMKYTQNNQSKAAQWLGLNRNTLRKKLLEHKLIN"
BASE COUNT         2697 a         3529 c         3307 g         2384 t
ORIGIN      
        1 ggtggcattt gtccaaattc acattttttt cgagtggctt cagcaccttt ggggctttcc
       61 cggtttaagt ccgacaggct gctagataaa tttaatcatc aacttctaac ccgaacagcg
      121 ccaaaatctt aaatctctac taccgcttcc tggcttcacg actcatgcat ggccaaaacc
      181 ccaacgactc ctgacgctgc ggtgccgacg aacaccgccg aaaactgatc gcccccatcg
      241 aacgaccggt tccaagttta ggggccgtga aaatgatgaa attggcctca cgacggcaat
      301 gtccgctttg gagaaatcga tgaaattcca gggttgaccg cgaccgtccg catacgctgc
      361 agtgccgaca gccaccggca aaaatcgatc aatcccctcg aatgactgcg ttcgaatttt
      421 ggatcggggc caatgacgaa attggagcca cgtcggcgat gaccgctatg gagaagtcga
      481 tgaaattcca gtgttgaccg cgaccgtcgg caatcgctgc agtgccgaca gccaccggca
      541 aaaatcgatc aatcccctcg aatgactgcg ttcgaatttt ggatcggggc caatgacgaa
      601 attggagcca cgtcggcgat gaccgctatg gagaagtcga tgaaattcca gtgttgaccg
      661 cgaccgtcgg caatcgctgc caaccggtca acagactaag ccatcgcgtt gacctgcgtg
      721 cccaaattgc cactcgtggc caacggcagg gcctgtagca acgccaacgc accggatgcc
      781 tgtaagtcca tggtcttctc caaaaccaaa acccgggccg tttcggtgac ttggctttgc
      841 tccatttgtc tcgatgcggc aacgagcgcg gtgaggggaa tatccatggc gaaatccttt
      901 tgtggctgtc accaatatcg gttctgccaa cgacgttctt caacggtttc tcgtagcaaa
      961 gtgcgtccat accgtgtagc cacaacggca aaaacgcact gatttagggc attcttcgtt
     1021 ctcagtcgct cagacctacc aaaacacttt ccaaaccgca cccgcgtaag tggttgattt
     1081 tattgaaggt ggtctcttga atagtcaaga gacaaacaga gaacagagag agaaaacgcg
     1141 gttttttggg ccaaaaacgc ccaaaatgac tcaaattcag gagcagaaca ctcaagaggc
     1201 agttgccgta agtctttgaa aacgttaggg attttggaaa agaaaagcca aactgaaaag
     1261 agtttggcta aaaacgactg gtggtgatag gtggatttga accaccgaca tcagcattat
     1321 gaatgctgcg ctctaaccaa ctgagctata tcaccaacgg agccgaatta tagcgagtag
     1381 aaacaaccgc cggcagcgac actatttgtg tgtgaaaacg gctttgcgct tgttgacaaa
     1441 cgcatgcatg ccttccttct gatccgcagt ggcaaacaag gcgtgaaaca ggcggcgctc
     1501 gaacatgatg ccgtcggcca aaccaccctc aaaggcccgg ttgaccgatt ccttgaccgc
     1561 catggtggca atttgcgagt attcgctgat catcagggcg gcacccaggg cttcttccat
     1621 cagcttgtcc aatggcacga cgcggctgac cagtccggcg cgttcggcct cattggcatc
     1681 catcatgcgg ccggtcagcg ccagatccat cgccttggac ttgccaaccg cgcgcggcag
     1741 gcgttgtgtg ccaccggcac cggggatgat gcccagcttg atctcgggct gaccaaagcg
     1801 ggcgttatcg gcggcaatga tgaagtcgca catcatcgcc agctcgcagc caccacccag
     1861 cgcaaagccg ctgacggcag caatcaccgg cttgcgcacg ctgcgaattt gttcccagtt
     1921 gcgggtgatg aagtctccct tgtacacatc ggcaaaactg taggtcgcca tgccgccgat
     1981 gtcggccccg gcggcgaacg ctttttcgct accggtgatg atcatgcagg ctattgacgg
     2041 atcggcatca aaggcctgca atgcagcgcc cagttcagtc atgagttgat cgttcagcgc
     2101 gttgagctgc ttggggcgat tcaatgtcac gataccgact ttgtcagctt cggttcgcgt
     2161 ggtgataagt tcataagtca tggtgatact ccttgtggaa atcgtcaaat gcgctttgcg
     2221 cgggttcggc aggttcagtg ttgcccagcc aaagaccgac cagtttggca tcgtcgatgc
     2281 tgagtttgac attggcaacc gtgtcgtcaa gagtaacttc aagcgtttgc aggcgttggt
     2341 cgcgcgcaat ccaggccttg aaggttctcg actgacccag gtaaaggggc aaatcgtcaa
     2401 gtttggccag tcgccaacct tgcgcggact tgtcctggcc aacttcgatg ccaagccatt
     2461 cgtcgcccgc cgcaaaaccg gccagttcgg cggcgctgcc tcgcagcaca ctcttgatgc
     2521 gaatgccgct ttgctcagcc acccgcaagc caagttgatc gccgattgcc gctgcttcct
     2581 ctctgtaaac cacaccttgc cgtgtcagca atggcttgag cggcaggtcg ccagtgccat
     2641 gcacccaggc tgcaatttgc gcctcaaatg actggcccga cagctcgacc agcactgcca
     2701 gcaagtcgtc ttgcgtcatc gggccacccc ggcagagctg ccacagcctg cgcataaccg
     2761 catccaggct ggttttgccg ccctggcgca agctaaggtc caggcacagt gccaccagtg
     2821 aacccttggt gtaatagctg atggtggcat tggcagtgtt ttcatcctgc cggtagtact
     2881 tgacccaggc atcaaagctg gcctgggcca cggtttgcaa atgccgtccc ggggtctgca
     2941 gcacctggtt aatggttttg ttcagcaggc tcagataagt gccgtcgtca atcaggccgg
     3001 cacggcgcag cagcaggtca tcaaaatagc tggtgaagcc ctcaaaaaac cacagcagtt
     3061 cggtgtagtt ttcctggttg tagtcatagc ggacaaactc ggccgggcgc aggcgcttga
     3121 cattccaggt gtgaaagtac tcatggctga tcagtcccag cagcgtgaca tagccgtctg
     3181 ccactgccag cggtttggtt tgcccaagcc gtggcaagtc ggcgcgctgg caaatcaacg
     3241 cggtcgagtt gcgatgttcc agcccgccat acccattgtc cacagcattg agcatgaaca
     3301 ggtaatggcg gtggggcggc atcatttttg ctgcacggtc tgttcccggc gtggtttcgc
     3361 catgccagaa gttgacgctg gcttcacaaa ttgtccgggt atcagccagc agccttgcgc
     3421 catcaaaggt gggtgccgca ccggccacca caaaccggtg caggatgcca aaggccgtga
     3481 atgaaccgca ccagaaccgg cccatttcga ccggcgagtc caccagttca tcgtaattgg
     3541 cagccagata ggtgccaaaa ccgtcagggc ctgattgctg gggtgtcagc gatgtcgcaa
     3601 cctgccaatc tgacggcaca ccctcagatt caagcgtcag gcagtgctct tcttgctcct
     3661 ggccatgcac ccgcaggcac acgctggtgc cattgaaaaa accgcgccgg gtgtcaagcc
     3721 aggcagtgcg caccgagttg tcaaaagcgt aaacctggta acccagcacc aacggttgac
     3781 ctgcaacgca gtgcacctgc caggtgcatt tgtcgcgctg cgtcagtggg acatcactcc
     3841 ccgcctggcg cgcctgcagg cactgcagat ttttggaaaa ctcccgcacc agatagctgc
     3901 cggcaatcca gaccggcagg ctcaccacct gcatggcggc gggctggttg atcgtcaggg
     3961 tgatgccaaa caagtgtgca tgcaggttgt gcatgccaat gcggtagtca acagcgctca
     4021 tggccgacgc tgttagccgc ccggctggct caggcgcttt tcgatctggt cggcaccgac
     4081 ggcaccggga acacgcgaac cgtcagcaaa aatcagggtt ggcgtgccgg atatcttgtg
     4141 ctttttaccg aactgcatat tgcggtcaac ggcggccgta tcacatttag cgctggctgg
     4201 ctccacatca tcgagcatcc agtcctgcca ggttttgcct ttgtctttgg cgcaccagat
     4261 gtttttggat ttctcaaccg agcccggtcc gagcaccgga tacaaaaaca tgtgaatggt
     4321 gatgttattg accttctgca ggctttcttc aaagcgcttg caatagccgc agttgggatc
     4381 ttcgaacacc gcgagcttgc gcttgccatt gccgcgaacg atggtgaagg catccttgac
     4441 cggcagcgca tcaaaggcga cagcggtgag tttgtccacc cgctcctcgg tcaaattgcg
     4501 cttttgccgg gtatcaatca ggttcccctg caccaggtaa ttggcttggg catcggtgta
     4561 ataaatttcc gagtcattca cgcggacttc atacaggcca ttgatcggtg ttttgctgac
     4621 ttcatcgatc ttttgcagtt gtggcacacg ctcagccaga ttcttgcgaa tgctggcctc
     4681 ttgcgcccag acgccggtgc ccgccagcaa cccgcaggca agcacagtgg tgttgaaatg
     4741 tttcatcatc ttccagtcaa aaatccatta gagccccatg gcgtagcggg ccgcccagtc
     4801 cttggccaga ccgctgtgct cgaagccgtt catgccccag ttgcgcagcg cctgccaggt
     4861 gctgccgtct ttggagaaca attgctgcaa gccgtccatc gccagcgccg ttgcgctgac
     4921 gtcgctgcgc cgggcccgct cgtagcggcg caaaagtcga gtgtcgctga ttggccgcca
     4981 gtattcgcgc ttgtgcagga tgctgaccag ttcagccaca tcacccagcc caaggttcag
     5041 gccctgcccg gccagcggat gaacgctgtg cgccgcatct ccggccagcg cccatgcctg
     5101 gccattggca acaccaatcc agcgactggc ctgggccgat tgcagtggcc aggcaacgcg
     5161 tgggctacac aaactcaact tgccctgaca gtccttgctg atttgcgcca actcggcgca
     5221 aaaaacctct tcgtcggcat caagcaggtc ttgggcacgc gactccagca cagaccagac
     5281 caatgccacc gcactgccct gctcgccatc cagcggcaaa aaagccagaa tctcgcccgg
     5341 tgtaaaccac tgacgggcaa cctgggcatg cggcttttca caggtcaagc gggcagcaat
     5401 ggctcgctgc gggtaacggg cgacggcgaa ttcaacgcca aattcttccc gggtgcggct
     5461 ggccttgcct tcacagacca ccgtcaaggc ggctggctgg gggctgtcca acagttcaat
     5521 atgtggctga aaacgcaccg catcgctcaa ccgggcttcc agcgtgggca catcgacaat
     5581 ccaggtcaat gctggcaccg ccaggtccac cgcatcaaaa ttgacttcac cgccctcatc
     5641 acccagcact cgcatgctgg tgacggcagt agcgtgcagt tcatcgggcc agcaacgcac
     5701 cgactcaagc agcgctttgg acttggcatt gagcgcatag gcgcgcacgt cagtggatgc
     5761 cggttttaac ggttctgctg ctggcgattg caccaccagc ccgacccgca gccggtcgcg
     5821 cgccaacaac aaggccaagg tgtaggccac aatgccagcg ccgcggatgc aaatatcaaa
     5881 ttgatgcgtc atgatggcaa ttgtaggagc gccgtttgac ctcatggata atcgggggtt
     5941 tggctttcgg gggtctatct tgcttgctat ggcagcgtca tagcgccccc caacttactc
     6001 aggactccta tgaaatgtgg catcgtgggc ttgcccaacg tcggtaaatc gaccctgttc
     6061 aacgcactca ccaaggccgg tatcgcggcc gagaactacc ccttttgcac catcgagccc
     6121 aatgtcggca ttgtcgaggt gcccgacgtg cggctggatg cgctggccgc catcgtcaag
     6181 ccgcaaaaga ttcagcgcgc cattgttgag tttgttgata ttgccggcct ggtggcaggt
     6241 gccagcactg gcgaagggct gggcaacaag tttttggcgc atatccgtga aaccgatgcc
     6301 accgtgaatg tggtgcgctg ctttgaagat gacaacgtgg tacatgtgtc gggcaaggtt
     6361 gatccggtct cggacatcga agtcatccag accgaactgt gcctggcgga cctgagcacg
     6421 gtggacaagg cgctgacacg ttatcaaaag acggccaagt ccggcggtga caaggaggcg
     6481 gccaagcttg tcacgattct ggagcgttgc cagaccgccc tgaaccaggc caaaccagtg
     6541 cgcgcactgg acttcagcaa ggaagagttg ccgctgctca aacagttttt tttgattacc
     6601 gccaagccag ccatgtttgt cgccaatgtg gctgaggacg gttttcagaa caacccgttt
     6661 ctggaccgcc tgaaggcata cgccgatgcg caaaatgcac cagtggtcgc catctgcgcc
     6721 aagatggaag ccgaaatggc ggacatggga gaagaagaca aaaagatgtt tctcgccgag
     6781 attggccaga ctgagccggg gctgaaccgc ttgatcatcg ccgcctacaa actgctgggc
     6841 ctgcaaactt acttcaccgc tggcgtgaag gaagtgcgcg cctggaccat tcatgtgggc
     6901 gacaccggcc cgcaagccgc tggtgtgatt cacaccgatt tcgagaaagg ctacatccgc
     6961 gcccagacca tcgcttttga tgatttcatc aacttcaagg gcgaacaggg tgccaaggac
     7021 gctggcaaga tgcgcagtga gggcaaggaa tacatcgtca aggacggcga tgtcatgaac
     7081 ttcctgttca gcccctgatt tataagaaat agggctctag gcctttgtga ataagcgctg
     7141 gcagctactt tatcaatagc aatatagaga actgaatgcg gcactgggca agggtttagc
     7201 cgccaaacat ccccaccaaa gcggccgtca tcgacaggta gcgcgcaaac ttgccgatgg
     7261 ccatgtaggc aaggcatggc cagaacggca gtttcagcca accagccacg gcacataagg
     7321 ggtcgccgac caatggcagc caggccaaca aacaggcttt gggccccagc tttttcagcc
     7381 aggccagcgc ccgcaaatgg tgtgaagaat ggcgataccg gtcaaccacc ttgtgcgagg
     7441 ccagccccat ccaccaactg accgcacctc ccagcgtatt gcccagggtg gcgatgccaa
     7501 tggttggcca aaacagatgt ggattgagct gaaccaaccc aaacaccgca ggctctgacc
     7561 ccaaaggcag cagcgtggct gaaatgaacg acaccacaaa aaccgtactc agcccaaact
     7621 tcggcaaggc cagcagcgtc agaaactgtt ccagccaggg ctccatctca caagcccgct
     7681 tgccgttttc gactgatcag cagatcacgg tcacagactt gatgcaaaac gcgatggtgt
     7741 gaaagtggca aaaagaccag acccgttgcg gatgactgtc agacagtcag cctgaacccg
     7801 cattcaccac agaacatatc gcctgccaga tgaggcgcat ggcatcgcgg acagttgaga
     7861 ctggcaactg gcgcaaccgc gattggcgca atggcgacct gaggtgcgtc agcccgagct
     7921 tgggcttggg cttgagcttg agcttgggct tgggcttgag cttgagcttg agcttgagct
     7981 tgagcttgag cttgagcttg agcttgagct tgggcttgag cttgagcttg ggcttgggtc
     8041 tgagcagcag cggctttcgc tcgggcttcc tgagcgcgct gccgcgcttc gttgaccttg
     8101 tcctgaatgg atgcttccat gcctgccaca tcgacgcctt cgaggttggc cagataaaca
     8161 ctgcagcaac cccgcgagaa aaccagcgag gccagactaa accccacggc ataaacaatg
     8221 cccccgccaa ccgacgcggc aacaaaatag ccattgccct caaaaccact gctgagcaag
     8281 tcctccatgc cgccaaaacc cacaccgatg ttgagtattc cagccgccat gccagcagtc
     8341 accatggtac caaccaccag gattgaaccg accgtgaagg tgaccgccag cacgatcagg
     8401 gtcaacacga tcatcatcag catcacattg accaccctgg cgcgcgcgat ggcaccgacc
     8461 cgggacaccg cctgcatggt ggtggcaccc gcccagatgg ccggcgcggt caagggcagc
     8521 accaccattt gcagcgcaaa cacggcaacc cctatcaaca ccactgacaa gggaaaaaca
     8581 aaggtgaaaa agaacggccc gatgaacggt actttgcaaa tcagcaacaa aagcgcaatc
     8641 gccagtagtc ccagcacata gatcaggcca aacagcaaga aggccaaaat caaccggtga
     8701 ccagtggcca acgactccag cacggccgcc agcacattgc gcgaagcaaa cccacgcgct
     8761 tcatccatca gcatgatgcc aacagcgttg acaccgtaaa agaacgtggc tagccccacc
     8821 aggaagaaca ggaagccaac ggccatgtga accttgaccg acaacaggcc acccaggccc
     8881 caaaacaggc ccgcagcaag aagcgcggca aacatcagcg ccaccgctcg ccagttgcgc
     8941 actgaatcga cggcgctcaa cagggttcca accttcacgt tttgcatggc tgacatgaca
     9001 aacctttctc gctaattagt ccactttgac agataccgat cagttgtccc gaagacacct
     9061 gcaccatcag cgaccgccgt cccgccgttg tgccgcctct tgctgttgca ttcgcatttc
     9121 aacgcattga gggtgattga cgaaccgacc cgagttgcat tcattctgca tgcaaaaggc
     9181 cagcgccaga aaactgcgct tgccacaagc gtccctgggt gatgccgacc tgatcggctc
     9241 agtcgccacg ggcggtactt gctgggctgg cgcaacataa ggctctggcg aagttgacct
     9301 tggctcgggt tcggccattg gctgcttttt caccaccggc ggttttgctg tcggcttggg
     9361 tggcttgact ggctggtcac tgggcatctg gttggcagcg ttgttattgg gcactgctgc
     9421 tggctctggt gttgccgctg gcatcggtgc aggaaccggt gctgtttccg tgatgatcgc
     9481 tggtggcgca acggtcggcg ctgctgcgct actagctggc tcgacagcaa cgggcgctgg
     9541 ctggacaccc gcagacaacg ctgccgagct gggcgctgca ctggcattca ggctgtcggt
     9601 tgatgccgcc cgctggctcc agaaccagcc acctgccgct atcgccagtg caaaaaacaa
     9661 cgcaccagcg gccagccata tcacgttgga tgacgctttc ttgacaggtg gttcaaccgg
     9721 cagggcattg cgaggcacgg cggccttcgg cggctcgtca aactgactca gatcaatctc
     9781 tggcatgaca cccttgtgcg gggcagtcaa cgctggctca ggtgcgcgca ccggcggtat
     9841 cggcacaggt ataggttcag gaaccggtgc aggcgcagga accggcagta ttggtctagg
     9901 taccggcact ggtgcaggtg caggtgcagg tgcaggtgat gccacgggtg atgcatctgc
     9961 ggcgaaaacc accgttgcgt ccaaatcaac tggagcgggt gacagaccgg ctggaacgcc
    10021 tgctgatgcc aagctatagc cgcacttctt gcaaaatttt gcctgctgca cattgagcgt
    10081 atggcattgc gggcaactat tcatcttgac ggtatcgagg tctgagccgc ctgtgcttgc
    10141 cgcaacggcc agcgatgtgc cacatcccag acaaaacttg gcattgtcac ggttgctttt
    10201 accgcatttc ttgcattcaa tggacatctt ggttcacctg cttgttttga ctcttgcgaa
    10261 gctgacacct tggggcccca aactttacat cagtttcgcc gggtccttgt agcagcgcag
    10321 ggacccgtgg tatttgcagc aaaagcatga aaccacggca gtcttgctga aattttaagc
    10381 agctaaaatt cagccgcgct gagcctcttt ttctaatttc ctgcagtatt tcccgcattt
    10441 tttcgcatga accttggtaa ccaccgtttg ctaaaccccg tctttgtcgc cccgatggcc
    10501 ggggtgactg atcgcccgtt tcgccaactc tgcaaacgtt tgggggctgc ttacgcggtg
    10561 agcgaaatgc taagcagccg gccagacctg caacactcgc caaaaaccac acgtcgtgcc
    10621 aaccacgcag gcgagcccgg cctgattgct gtgcagattg cgggcaccga tccggccatg
    10681 atggccgatg cggcacgcct taacatcgac cgcggcgcgc aggtgatcga catcaacatg
    10741 ggctgcccgg ccaaaaaggt gtgcaacaaa tgggctggct ctgccctgat gcaagatgag
    10801 cgcctagcgt tacagattgt tgatgcggtg gtgcaagcct gtgcaccgca cggcgtgcca
    10861 gtgacgctga aaatgcgcac cggctggagt caggcgaaca agaacgcggt gcagatcgcc
    10921 cgtctggccg aactggcggg ggtgcagatg ttcaccatcc atggccgcag ccgcgaacaa
    10981 ggctacctgg gacaggccga atacgacacc ttggccgccg tgaaagccat ggtgcgtgtg
    11041 ccggtggtgg ccaacgggga catcgacagc ccgcaaaagg cccgcgcagt gcttgcagca
    11101 accggtgctg acgcggtgat gattggccgc gcggcccagg gtcggccctg gatttttcgg
    11161 gaaattgtcc acttcattgc caccggtgaa acgctcgcgc agccgctggt cgccgaggtc
    11221 aaacaactgc tgttggcaca tttgcaagac cactacacgc tatatggcga gcatgtcggc
    11281 ctgcgcacgg cacgcaaaca cattggctgg tatgttaaaa ccctgcccgg cggcgaagcc
    11341 tttcgggccc acatgaacgc cctggaagac tgtcgcttgc agcatgacgc ggtggctgac
    11401 ttttttgatg aactgaacct gcacatggac cgcctgccaa cgccaacagt aaccgatgta
    11461 acccataaca aacagaacca accggagaca ctcacttgaa accccctcac gtcgcagact
    11521 gtgtgcgcac cagcctcgaa ggctatctga gcgacctcgg tagcagcgaa cccaaccgca
    11581 tgtacgagat gctgatgagc acggtagaaa agccgttact ggaagttgtc atgaagtaca
    11641 cccagaacaa ccagtcaaag gccgcgcagt ggctcggcct gaaccgcaac accctgcgca
    11701 aaaagctgct ggaacacaag ctgatcaact aggcgcccgt cgtacctatg gttcaagcaa
    11761 ccaaaagcgg ccctggcttg gctggtttgc gccggatttg caggcccata gcccagttgc
    11821 gggtcaaaaa ccccggtgca aaatgggtcg ttgcggtcga ttcgcagacc aacgcaccat
    11881 aagccaaaga agctcctgaa cagctatggc acagccc
//
LOCUS       DAOSLL010000060        11901 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5381_length_11901_cov_4.856070, whole genome shotgun sequence.
ACCESSION   DAOSLL010000060 DAOSLL010000000
VERSION     DAOSLL010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11901
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5381_length_11901_cov_4.856070"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="PLB25_04090"
     CDS             <1..806
                     /locus_tag="PLB25_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease domain-containing protein"
                     /protein_id="HOE40798.1"
                     /translation="NGYNWLGEPVYNPFALLQLFRSRKFMPHWFETGTPTFLIKLLQQ
                     RQQFTPDLSRVVASAALLSTFDVDNIPVEALMFQAGYLTIKEVWQIPGQMELTLKYPN
                     LEVQAALNNSLLQSLTGDLAVPGPQISRLYKLLTATDFAGLKALFHAFYASIANDWYR
                     KNELSSFEGYYVSIFYSYFAALGLDIRLEDPSNFGRIDMTVLFDGQIFLFEFKVVELN
                     PAGAALQQIKDMHYADKYQGLGQPIHLIGVEFSKASRNIVGFEVETLSS"
     gene            complement(1271..1873)
                     /locus_tag="PLB25_04095"
     CDS             complement(1271..1873)
                     /locus_tag="PLB25_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE40799.1"
                     /translation="MLGEWVIDTALTQVERWRAAGLELGVSVNIGARQLQQADFVDRL
                     QALLAQHPQVQPGWLELEILETSALSDMAQVSSVIAACAAIGVNFALDDFGTGYSSLT
                     YLKRLRVVLLKIDQSFVRDMLVDPDDLAILQGVIGLAAAFKRQVIAEGVETVEHGSAL
                     LQLGCEQGQGYGIARPMPGEQLPAWVASWQPDVAWRCADR"
     gene            complement(1855..4794)
                     /locus_tag="PLB25_04100"
     CDS             complement(1855..4794)
                     /locus_tag="PLB25_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE40800.1"
                     /translation="MKHPNPGTANTALTLTEAAALRQKAEAAWQQIASESIDAFEDLT
                     IAARRSLLQELQVHQIELAMQNEELRRAKLELETSRAGYMNLYELAPVGYCSVTETGL
                     LLQANLTLAKMLGAARGDLAGQPFSNFVLKADQDLWYRLHRQLLDSALPQAGELRLRL
                     PDGAPLWVNLSATLAQDDSGAQVLRVGVSDINARKQAEAALSESDARWKFAVDGLGDG
                     LWDWHVQTGAAYYSQRYKEMFGYADADIGASANEWSQRMHPDDAPGVMAALQPCFDGE
                     ADVASVEFRMLCKDGSWRWTLGRGMVMSRDAQGRALRLIGTNTDISERKQGETVDQFL
                     AQTFGDPQAEPFFVTLARFLADSLHMDYVCVDRIDKDHLNATTLVAWFDGKLVDNQSY
                     ALADTPCAEVIGQPVRCFVSQVCQQFPKDLLLQELQAESYIGATLWSHIGEPIGLIAM
                     MSRHPLRNQAYARTTLTRVAVRTAGELERLDAEAALHISEARFHQLLNDIPLVSVQGY
                     DEDGITQYWNQASEHLYGFSATEALGRSLLDTIIPAEMHEGVRQGMRQMFETAMPIPA
                     GELSLRRKDGSRVDVFSSHAYVQVPGQRPEMFCVDVDLTERKQAQARLQLAASVFSHA
                     REGITITDADGRILEVNDAFTRITGYGRDEVIGQNPRILKSGRHDEAFYNAMWRELLS
                     NGHWSGEIWNHRKDGEAYAELLTISAVHDSDGQIRQYVALFSDITAIKAHQNALERLA
                     HFDTLTQLPNRLLLSDRLQQGMAQAQRRGQQLAVAYIDLDGFKSINDAHGHDAGDLVL
                     MALANSMKDCLREGDTLARIGGDEFVAVLMDLNDTSACLPLLNRLLAAAAAPVALGEH
                     VPQVSASVGVTFYPQAQPIEADQLLRQADLAMYQAKLAGKDRCHVFDAAQDISLRQHH
                     ESLERIRQALELGEFVLHYQPKVNMRSGLVIGAEALIRWQHPERGLLAPALFLPVIRW
                     RLCWVNG"
     gene            complement(4791..7874)
                     /locus_tag="PLB25_04105"
     CDS             complement(4791..7874)
                     /locus_tag="PLB25_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOE40801.1"
                     /translation="MTNSPKSKPAAPGQAASTPNLVPPTSMNDAKAEAAEVLAAPAFP
                     IVGIGASAGGLVAFQAFFSGLPANADPGMAFVLVQHLAPDHKSLLAELIARCTRMPVF
                     EVQDGMRVQINCVYVIPPNCEMTLQQGALHLHAPVVPRGKRLPINGFFQSLALDLQAH
                     AIAIVLSGTGSDGTLGVRAIKEAGGMVMAQDPPSCEYDGMPRNAIATGLVDYQLPPAD
                     MLAQLIACVAHSASLSLNPGRRAKSRLKSVLGQIFALLHSQTGRDFSNYKASGVQRRI
                     AKRMALQQIETPELYVNYLRDSATERQALFHDLLIGVTRFFRDPKAFEVLENQVIPKL
                     LANKPADGVVRVWCAACSTGEEAYSLAMVLQEQMERLNLSRTVQVFATDIDNRAIARA
                     RTGLFPAGIAANLSPQRLARFFVPEPAKAGYRVCKSLRDLLVFSEQDVVKDPPFSRLD
                     LISCRNLLIYLDAHLQKKLFALFHYVLNPQGCLFLGGSERVGDFAASFATIDHHARLY
                     QRQDDQSGARYGAARRSRVLAAAVDATLAGLSDLQRPALPVKPVKPLLRELVEQMLLQ
                     QLAPASALVNAAGDIFYLHGRTGLYLEPSAGEVRTQNLLKMARPGLRHRLGNALHQAI
                     ASGQTVQVTTVRVKTNGHDAYVNLSVRPAPIVAGAEDEEDAQRAAPLYLVTLEDAPQP
                     VDALTAPTDVAPGFEGATQARCAVLTQELLAKDEYLQSVHAELESANEELMSSNEQLL
                     SVNEELQTSYEEMETAKEQLQSFNAELHAVNTRLLTRVAEITHANNDMNKMLLGTGIG
                     TVFVDLQLNILRFTPEASRITNLIASDVGRPVAHIAFNLLGYNSLVADLQAVLGTLIP
                     KEAEVHAPDGQWYTLHIRPYRTLANEVEGAVISFVNITEVVRMREVLRQANHQQSLAA
                     VVVRDTHDAVTLQGLDGRTLAWNPGAVRLYGWSEQEALNMNVRERIPEGLRPGALARL
                     TQLGRSEALQAYHTQRLTRSGAVLQVSIVPTVLINEAGQVYAIATTERALPEDSA"
     gene            complement(8770..9195)
                     /locus_tag="PLB25_04110"
     CDS             complement(8770..9195)
                     /locus_tag="PLB25_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOE40802.1"
                     /translation="MDETDQQLLSMLRQDARLSVAALAQKLKVSRGTVTNRIRRLEDD
                     GIIVGYTVRLRPDVQHNDIKAWMSIAVDGNQTRAVIAALLGEPGLATLHDTNGRWDLL
                     AELRAESLQELAKVLERIRLLKGISNTETSIHLETYRLS"
     gene            9332..10519
                     /locus_tag="PLB25_04115"
     CDS             9332..10519
                     /locus_tag="PLB25_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase"
                     /protein_id="HOE40803.1"
                     /translation="MSRSTPTHRLTQTETLFVSPQDICHLVRSQGMAATLQGMAAYIH
                     QDFLRWQSFDKAPRVASHSSDGVIELMPIADASTYTFKYVNGHPKNTARGLSTVMAFG
                     VLADVSTGEPLLISELTLCTALRTAATSAVAARALARPDSRVMALIGNGAQGEFQALA
                     FHHLVDIEEIRLYDIDPLATAKLMHNLQDNPHAPGLRLIACSSIAEAVRGADIVTTIT
                     ADKTNATIISADMLEPGMHLNGVGGDCPGKTEMHADVLRAAKVFVEFEPQTRIEGDLQ
                     QMPPDFCVTELWQVLSGQKIGREGRSQVTLFDSVGFALEDFSALRYVHDQARTLGLGA
                     PIALIPQLADPKDLYQMLRPAGLKAVNTRCLSWTRPSGPLAARPSRAKAAAPNVPAHL
                     WTA"
     gene            complement(10889..11407)
                     /gene="lptE"
                     /locus_tag="PLB25_04120"
     CDS             complement(10889..11407)
                     /gene="lptE"
                     /locus_tag="PLB25_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HOE40804.1"
                     /translation="MFTHLHQRRRVAAALLLTGLLAGCGFKLRGSQTYAFSSIAITPF
                     PGEAVAQELRRSFGSAVRVLDKDTPLAQAQVVLAMQGEQREKVVVGVNASGQVRELQL
                     RIRVKFRLTTPQGQELVAADEILQQRDISYTESAALAKETEEAQLYRDMQADIVQQML
                     RRLATVKLPPSP"
     gene            complement(11552..>11901)
                     /locus_tag="PLB25_04125"
     CDS             complement(11552..>11901)
                     /locus_tag="PLB25_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOE40805.1"
                     /translation="VALIEGFGILLRCLYPATPHITHSLWSSLGYAGHLGDLLDAPWP
                     QVDPQALLQDEIELMLQVNGKLRGSIVVSASADQATIKQAAIAHEMFQKFSNGAAPKK
                     VIVVPGRLVNLVV"
BASE COUNT         2298 a         3681 c         3529 g         2393 t
ORIGIN      
        1 acaacggcta caactggctg ggcgagccgg tctataaccc gtttgcgctg ttgcaactgt
       61 ttcgctcgcg caaattcatg ccccattggt ttgagactgg cacacccact tttttgatca
      121 aattgttgca gcagcgccaa cagttcaccc ctgatttaag ccgcgtcgtc gcttccgctg
      181 cgctgctgtc gacctttgat gtggacaaca tcccggtcga agcgttgatg ttccaggcag
      241 gttacctgac catcaaagag gtctggcaaa tccccggtca gatggaattg acgctgaaat
      301 accccaacct ggaagtgcag gcggcgctca acaacagcct gctgcaaagc ctgaccggcg
      361 acttggcggt gccggggccg cagatcagcc gactctacaa actgctgaca gcgacagact
      421 ttgccggcct caaagccctg ttccacgcct tctatgccag cattgccaac gactggtatc
      481 gcaaaaacga gctgagcagc tttgagggct attacgtcag catcttctac agctactttg
      541 ccgcgctggg tctggacatc cggctggaag accccagcaa ctttggccgc atcgacatga
      601 cggtgctgtt tgacgggcaa atctttttgt tcgaattcaa ggtggtcgaa ctcaacccgg
      661 caggcgccgc gctgcagcag atcaaggaca tgcattacgc cgacaaatac cagggcctgg
      721 gtcaaccgat ccacctgatt ggcgtggagt tcagcaaagc cagccgcaac attgttggct
      781 ttgaggtcga gacgctatca agttaatagc tgccagcgca atcagcacaa gggctgcagg
      841 cactttttgc caataatcga cggcagttga atgaactctc tgacgatgcg ccctgcaccg
      901 cccgacctga ttgaattgaa tccgctggga aatttcaatg aaaactggtc ttaagccgcg
      961 tggcgtatgc gctgccagct attgtgttaa tagcattcag gggcactggc ccgtgatatt
     1021 ggggtctgag cccaattgct tttggcggct gctttctgtg ctaggccggt gagcacgcaa
     1081 ttgggatccg accccaaaat cactacaccg gtcgggcatt gatgcttgat ggcccagttg
     1141 tggcgaaatt ggggcgaaat tggggtcaga ccccaatttc accggccagg cggcagagtc
     1201 atcgcgatgc ctgccaaagc gtcgccgctc accagcaccg ctgttggctt tttctcatcc
     1261 atccaccgat tcaccgatct gcgcagcgcc aggccacatc aggctgccag ctcgccaccc
     1321 aggcgggcaa ctgctcgccg ggcatgggcc gggcaatgcc gtagccttgc ccttgttcgc
     1381 agcccagttg caacagcgcg ctgccgtgtt ccacggtttc taccccttcg gcaatcacct
     1441 ggcgcttgaa ggccgctgcc agaccaatca cgccttgcaa aatggccagg tcatccgggt
     1501 caacgagcat gtcacgcaca aagctctggt cgattttcag cagcaccacg cgcaggcgct
     1561 tgaggtaggt cagtgacgaa taaccagtgc caaagtcgtc cagcgcaaag ttgaccccga
     1621 tggccgcgca ggccgctatc acactggaca cctgcgccat gtcgcttagc gcactggtct
     1681 ccagaatctc cagttccagc caaccgggct gcacctgcgg atgttgcgcc agtagcgctt
     1741 gcagacggtc gacaaaatcg gcttgctgca actggcgggc accaatgttg acgctgacgc
     1801 ccagctccag cccggcggca cgccagcgct cgacttgggt cagggccgtg tcaatcaccc
     1861 attcacccag cacaaccgcc agcggatcac cggtaaaaac agcgctggcg ccagcagtcc
     1921 gcgctcagga tgctgccagc gaatcagcgc ctcggcccct atcaccaggc cgctgcgcat
     1981 gttcaccttg ggctggtaat gcagcacaaa ttcacccaac tccagcgcct ggcgaatgcg
     2041 ctccaggctt tcgtggtgct ggcgcaggct gatgtcttgc gccgcatcaa acacgtggca
     2101 acggtctttg ccggccaatt tggcctggta catggccagg tcggcctggc gcagcagttg
     2161 atcggcctcg atcggctgcg cctgcgggta aaaggtgaca ccgacactgg ccgacacctg
     2221 cggcacatgc tcgcccagcg ccaccggcgc tgctgctgct gccagcagcc ggttcagcag
     2281 cggcaggcag gccgacgtgt cgttcaggtc catcagcacc gcgacaaact cgtcgccacc
     2341 aatgcgcgcc agcgtgtcgc cctcgcgcag gcagtccttc atgctattgg ccagggccat
     2401 cagcaccagg tcaccggcat catgtccatg cgcgtcgttg atcgacttga agccgtcaag
     2461 gtcgatgtag gccaccgcca gttgctggcc gcgccgctgc gcctgtgcca tgccctgctg
     2521 caagcggtct gacagcaaca ggcggttggg caattgggtc agcgtgtcaa agtgcgccag
     2581 acgctccagc gcgttctgat gcgccttgat ggcggtgatg tcagagaaca gcgccacata
     2641 ctgccggatc tggccatcgg agtcatgcac cgcgctgatg gtcagcaact cggcatacgc
     2701 ctcaccgtcc ttgcggtggt tccagatttc gccgctccag tggccgttgc tcagcaattc
     2761 ccgccacatc gcgttgtaaa acgcctcatc atgtcggcct gacttgagaa tgcgcggatt
     2821 ttgaccaatc acctcgtcgc ggccatagcc ggtgatgcgg gtaaaggcat cgttgacctc
     2881 cagaatgcgg ccgtcggcat cggtgatggt gatgccttcg cgggcatggc tgaacacgct
     2941 ggcggcaagc tgcaacctgg cctgcgcctg tttgcgctcg gtcagatcaa cgtcgacgca
     3001 aaacatctcg gggcgctggc ctggcacctg aacataagcg tggctggaaa acacatccac
     3061 ccgagagccg tccttgcggc gcagtgacag ctcgcccgcc gggatcggca tggcggtctc
     3121 gaacatctgc cgcatgccct ggcgcacgcc ctcatgcatc tctgccggaa taatggtgtc
     3181 aagcaggctg cggcccaggg cctctgtggc gctgaagcca tacaaatgct ccgaggcctg
     3241 gttccagtat tgcgtgatgc cgtcttcgtc ataaccctgc accgacacca gcggaatgtc
     3301 attgagcagt tggtgaaaac gggcctcgct gatgtgcaag gcggcttcgg catccagccg
     3361 ctcgagttca ccggcagtgc gcaccgccac ccgggtcagc gtggtccggg cataagcctg
     3421 gttgcgcaag gggtggcggc tcatcatcgc aatcaggccg atcggctcgc cgatgtggct
     3481 ccacagggtg gcaccgatgt agctctcggc ctgcagctcc tgaagcagca aatctttggg
     3541 gaattgctga cacacctgac tcacaaagca gcggaccggt tgcccgatca cctcggcgca
     3601 gggtgtgtcg gccagggcgt aactctggtt gtccaccagc ttgccatcaa accaggcgac
     3661 caacgtggtg gcgttaaggt ggtctttgtc aatccggtca acacagacat agtccatatg
     3721 caggctgtcg gccaaaaagc gcgccagcgt gacaaaaaag ggttcagcct gtgggtcgcc
     3781 aaaggtttgc gccagaaact ggtccaccgt ttcgccctgt ttgcgctcgc tgatgtcggt
     3841 gttggtgcca atcaggcgca gggcccggcc ctgtgcgtcg cggctcatca ccatgccgcg
     3901 cccaagcgtc cagcgccaac taccgtcctt gcacaacatg cgaaattcaa cgctcgccac
     3961 atcggcctcg ccatcaaagc agggttgcag cgcggccatc acgccgggtg catcgtcagg
     4021 gtgcatgcgc tggctccatt cgttggcgct tgccccgatg tcggcatcgg cataaccaaa
     4081 catttccttg taacgctgcg agtaatacgc cgcgccggtt tgcacatgcc agtcccacag
     4141 gccgtcgccc aggccatcga cggcaaattt ccagcgcgca tcgctctcgg acagcgctgc
     4201 ctcggcttgt ttgcgggcgt tgatgtcgct gacccccacc cgcagcactt gcgcaccgga
     4261 atcgtcttgc gccagcgtgg cgctcaggtt gacccatagc ggcgcgccat cgggcaggcg
     4321 caggcgcaac tcgcccgcct ggggcagcgc gctgtccagc agttgccggt gcagccggta
     4381 ccacaggtcc tggtcggctt tgagcacaaa gtttgagaac ggctgaccag ccaggtcgcc
     4441 gcgcgccgcg cccagcatct tggccagcgt caggttggcc tgcagcagca gcccggtctc
     4501 agtcacgctg caataaccca ccggtgccag ctcgtagagg ttcatataac cggcgcgcga
     4561 ggtttccagc tccagcttgg cgcggcgcag ctcctcgttt tgcatcgcca gttcaatctg
     4621 gtggacctgc agctcttgca gcaagctgcg gcgggcggca atcgtcaggt cttcaaatgc
     4681 atctattgac tcgctggcaa tttgctgcca ggcggcctcg gccttttggc gcagtgctgc
     4741 ggcctcagtg agtgtcaacg cagtattggc agtgccaggg ttcgggtgtt tcatgctgag
     4801 tcttccggta acgctctctc ggtcgtggcg atggcgtaca cctggccagc ctcattgatc
     4861 aggacggtgg gaacaatgct gacctgcagc accgcgccac tgcgggttag tcgttgggta
     4921 tggtaggctt gcagcgcctc actgcggccc aactgcgtca accgggccag cgcgcctggg
     4981 cgcagcccct cggggatgcg ctcgcgcaca ttcatattca gcgcctcttg ctcactccag
     5041 ccgtacaggc gcactgcgcc ggggttccag gccagggtgc ggccgtccag tccttgtagc
     5101 gtcaccgcat catgtgtgtc gcgcaccacc accgccgcca ggctctgctg gtggttggct
     5161 tgacgcaaca cctcacgcat gcgcaccact tcagtgatgt tgacaaacga gatcaccgcg
     5221 ccttcgacct catttgccag cgtacggtaa ggccgaatgt gcagcgtgta ccactggccg
     5281 tctggcgcat gcacctcggc ctctttgggg atcagggtgc ccagcaccgc ctgcaagtcg
     5341 gccaccaggc tgttgtagcc cagcaggttg aaggcgatgt gcgccaccgg tcggccgaca
     5401 tcggaggcga tcaggttggt gatgcggctg gcctccgggg taaagcgcag aatgttcaac
     5461 tgcaaatcga caaataccgt gccaataccg gtgcccagga gcatcttgtt catgtcgttg
     5521 ttggcatgcg tgatttcggc cacccgggtc agcagcctgg tgttgacggc gtggagttca
     5581 gcgttgaaag attgcaattg ttccttggcg gtctccattt cttcgtagct ggtttgcagc
     5641 tcttcgttga ccgacagcaa ttgttcattg ctggacatca gctcttcgtt ggcgctttcg
     5701 agctccgcat ggacgctttg caggtactcg tcttttgcca acagttcttg cgtgagcact
     5761 gcacagcgag cttgtgtggc accttcgaag ccgggggcta cgtcagtggg cgctgtcagc
     5821 gcgtcaactg gctggggcgc gtcttccaga gtgaccagat acagcggcgc ggcgcgctgt
     5881 gcatcctctt catcctctgc acctgcaacg attggtgccg ggcgcacgct caggttgacg
     5941 taggcatcgt ggccattggt cttgaccctg accgtggtca cctgaacggt ttgcccgctg
     6001 gcaatcgcct ggtgcagtgc gttacccagc cggtggcgca aacccggtcg ggccattttc
     6061 agcagattct gggtccggac ctcgccggca ctgggctcca ggtacaggcc ggtgcgcccg
     6121 tgcaggtaaa aaatgtcgcc cgccgcattg accagcgcac tggccggggc caactgctgc
     6181 agcagcatct gctcaacgag ctcgcgcagc aacggcttca ccggcttcac cggcaacgcc
     6241 gggcgctgca ggtcgctgag gccagccagc gttgcatcaa cggccgccgc caagacgcgt
     6301 gaacggcgcg cggcgccgta cctggcaccg gactggtcgt cctggcgctg atagagtctg
     6361 gcgtggtggt caatcgttgc aaacgaggcc gcaaagtcgc ccaccctctc agacccgcca
     6421 agaaacaggc aaccttgggg attgaggaca tagtgaaaca gcgcaaacag ctttttttgc
     6481 aggtgcgcgt cgaggtaaat cagcagattg cggcaactga tcaggtcaag cctggaaaaa
     6541 ggcgggtctt tgaccacatc ctgctcggaa aacaccagca gatcacgcag gcttttgcaa
     6601 acgcggtagc ccgccttggc gggctcgggc acaaaaaagc gcgccagtcg ctgtggcgac
     6661 aggttggcgg caatgcccgc cggaaacagg ccggtgcggg ccctggcaat ggcgcggttg
     6721 tcgatgtcgg tggcaaacac ctgcacggtg cggctcaggt tgagtcgctc catctgctct
     6781 tgcagcacca tcgccagcga ataggcctcc tcgccagtcg aacaggcggc gcaccagacc
     6841 cgcaccacgc catcggccgg tttgttggcc aacaatttgg ggatgacctg gttttcgagc
     6901 acctcaaacg ccttggggtc acgaaaaaac ctggtgacac caatcagcag atcgtgaaac
     6961 agggcttgcc tctcggtggc gctgtcccgc aggtagttga catagagttc cggggtttca
     7021 atctgttgca gtgccatgcg tttggcaatg cgccgttgca cgccgctggc cttgtagttc
     7081 gaaaagtcgc gaccggtctg gctgtgcagc agcgcaaaaa tctgccccaa aacgcttttc
     7141 agccgggatt tggcccgccg accggggttc agcgacaggc tggcggagtg cgccacacag
     7201 gcgatgagct gggccagcat gtcggctggc ggcaactggt agtccaccag accagtggca
     7261 atggcgttgc gtggcatgcc gtcgtattcg caggacggcg ggtcttgcgc catcaccatg
     7321 ccgccagctt ccttgatggc gcgcacgccc agcgtaccgt cgctgccggt gcccgacagc
     7381 acaatggcaa tggcatgcgc ctgcaagtca agggcgagcg actgaaaaaa gccgttgatc
     7441 ggcaggcgct tgccgcgcgg aaccaccggg gcatgcaagt gcagcgcacc ttgctgtaac
     7501 gtcatctcac agttgggggg aatgacgtag acgcagttga tctgcacgcg catgccgtct
     7561 tgcacctcaa aaaccggcat gcgggtgcac cgggcaatca gctcggccag caggcttttg
     7621 tgatcggggg caaggtgctg caccagcaca aaggccatgc cggggtcagc gttcgccggc
     7681 aggccagaga aaaaagcctg aaaagccacc agaccaccgg ccgaagcacc gatgccgacg
     7741 atggggaaag ctggcgcagc gagcacttcg gcagcctcag ccttggcatc attcatcgat
     7801 gtggggggca ccaggtttgg cgtgctggct gcctggcctg gggctgctgg cttgcttttg
     7861 gggctgttgg tcatgctggg ctccgaaaac tgggccatga tggctgcgct cgtccagtgg
     7921 gaacgacatg ttagtttacg ccttgcatca ggcggttggg ttgcaatttg ctgggggcct
     7981 gcggctgcgg gtgcggtgcc cgctgatccc gtgatgctat ttttaatata gctgccagcg
     8041 catattccac aggggctgca ggtctatttc tctctgacaa atgcgccaag cccatgccac
     8101 aagggctgca ggccgatgct tctccatggt gacactgttg ctggaagttg acgggaactg
     8161 cggcgggctg tgagccgcaa tgggcgctaa ccacatcgag gtggcctcaa tcgcccctgg
     8221 cccgtgaaat tggggtctga gcccaatttc ctgtaccacc tggtttctcg ccgcagactg
     8281 gtggcaggaa attgggatcc gaccccaaat tcactgcacc tgtggtctgc attgatgccg
     8341 tcattccggc tctcgccacg ggttggtgac aagaaattga ggtccagctc aaattcactg
     8401 cacctctggt cttcgttgat gccttcaatc cggcacatgc cgggctttgc caggctgggc
     8461 catgccagtt acattgccgc aagtttcgcc ttcagcgccc tgccctgtcg agcttcaacg
     8521 cccgagcggt gcagtgattt tggggtcgga tcccaattgc gcagtcactc gctcacaaca
     8581 gccagcagcg ccaaacgcaa ttgggctcag accccaatat cacgggccac cacccgagca
     8641 tcatcaaaaa ccatagctgc cagcgcatat tccacggggt ctgcaagccg atttctctct
     8701 caacattgcc cagagcgcag gccacaaggg cacaggccga tgctgcttca aattcaagcc
     8761 agacggctgc tacgacagcc ggtaggtttc caggtgaata ctggtttcgg tgttgctgat
     8821 gcccttcagg aggcggatgc gctccagcac tttggccagt tcctgcaggc tttcggcacg
     8881 cagctcggcc agcaggtccc agcggccgtt ggtgtcgtgc agggttgcca gccctggctc
     8941 gccgagtaac gcggctatca cggcgcgggt ctggttgccg tcgacggcga tgctcatcca
     9001 ggccttgata tcgttgtgtt gcacgtccgg acgcaggcgc acggtgtacc cgacgatgat
     9061 gccgtcgtct tccaggcggc gaatgcggtt ggtcacggta ccgcgcgaaa ccttgagttt
     9121 ctgcgccaac gcggccaccg acaggcgcgc atcctgtcgc agcatggaca gcagttgttg
     9181 atcagtttca tccattttgc taacttcgtc tgtcaaaacg gcaattatgt gactattttt
     9241 agacaaagtt gacgattgtt attgtcaacg cgcccggcca cacttaccac taacccattc
     9301 agaccagcgc attgccccca ggagacccag catgagccgc agcaccccca cccaccgcct
     9361 gactcaaacc gagacccttt ttgtcagccc gcaagacatc tgtcatctgg tccgcagcca
     9421 gggcatggcg gccacgctgc aaggcatggc ggcctatatt catcaggatt ttttgcgctg
     9481 gcagagtttt gacaaggcac cgcgagtcgc cagccactca agtgatggcg tgatcgaact
     9541 gatgccaatt gccgatgcca gcacctacac cttcaagtat gtcaacggtc accccaaaaa
     9601 caccgcccgt ggcttgtcca cggtgatggc ttttggcgtg ctggccgatg tgagcaccgg
     9661 cgagccactc ttgattagcg aactgacgct gtgcaccgcc ctgcgcactg cggctacttc
     9721 agccgtggcg gcgcgcgcgc tggcccggcc cgacagccgg gtcatggcgc tgattggcaa
     9781 tggtgcgcaa ggcgaatttc aggcgctggc gtttcaccat ctggtcgata tcgaagagat
     9841 tcgcctgtat gacatcgacc cactggccac cgccaagctg atgcacaact tgcaggacaa
     9901 cccgcacgcg cctggcctgc gcctgatcgc ttgcagcagc attgccgagg cagtgcgcgg
     9961 ggccgatatc gtgaccacca tcacggctga caagaccaat gccaccatca tcagcgccga
    10021 tatgctcgaa cccggcatgc acctcaacgg tgtcggcggt gactgccctg gcaagaccga
    10081 aatgcacgcc gatgtgttgc gcgcggccaa ggtgtttgtc gagtttgagc cgcaaacccg
    10141 cattgaaggt gacttgcagc aaatgccgcc cgacttttgc gtgaccgagc tgtggcaggt
    10201 gctgtcgggt cagaaaattg gccgcgaagg ccgctcacag gtcacgctgt ttgactcggt
    10261 cggttttgcc ctggaagact tctcggcgct gcgctatgtg catgatcagg cccggacgct
    10321 ggggttgggc gcgccgattg cgctgattcc ccagttggca gacccgaaag acctgtacca
    10381 aatgctgcgg cccgccggtt tgaaggcggt caacaccaga tgcctcagtt ggacgcgccc
    10441 gagtgggccg ctggcggcgc gcccgagtcg ggcgaaagcg gcagcgccaa acgtgcctgc
    10501 gcacctttgg acggcttgaa ccaagattcc tcagttaacc cgtcgctggt aaaggcgctc
    10561 aggggtcaag cgagaacaac ctggacaaag gtggtcgtga tggcaagtgc aaggccaggc
    10621 gcaggctgcg cggtgcaact ggctacacaa gcggtttgca acaacgcatt gcgcttgtca
    10681 gtgcggccga actgttcaag ttatttcctt gacaccttgg tctctaacaa gtcaagtctt
    10741 tgcaaatcat gcgaagaatt caaacattca aagtcattcg tcgcgagggc gcgactccta
    10801 caaattcaac taggcgccgg ctgtttgtgt aggagtcgcg ccctcgcggc gaaggccgtc
    10861 caattcaggc tcgtccggct tgtccgcctc agggtgacgg cggcagtttg acagtggcca
    10921 aacggcgcag catctgttgc acgatgtcgg cctgcatgtc ccggtacaac tgggcttctt
    10981 cggtttcctt ggccagcgca gcagactcgg tgtagctgat gtcgcgctgc tgcaagatct
    11041 cgtcggcggc aaccagttcc tggccctgcg gcgtggtcaa ccgaaacttg acccgtatgc
    11101 gcaactgcaa ttcacgtacc tggccagacg cgttgacacc caccaccact ttttcgcgct
    11161 gctcgccttg catggccagc accacctggg cttgcgccag tggcgtgtct ttgtcgagca
    11221 cccgcacggc actgccaaac gagcggcgca gttcctgcgc caccgcctca ccagggaatg
    11281 gcgtgatcgc aatgctggaa aaggcatagg tctgggaacc acgcagcttg aagccacagc
    11341 cagccagcag cccggtcagc agcagcgcag cggcaacacg acgccgttga tgcagatgtg
    11401 tgaatatggg tttcacggta ttcgttgaac ctggattcct cagttgaccg cttgttgtca
    11461 taggtaagtc cttatgggcg tttactttat tagcttcgat tggatttacc cattgggtca
    11521 cagcgctacg cacccgaccg ggcaagccag gttaaaccac caaattgaca agtcggccag
    11581 gcaccacaat cacctttttg ggcgcagcgc cgtttgaaaa tttctgaaac atctcatggg
    11641 cgatagcagc ctgcttgatc gtggcctggt cggcactggc gctgaccaca atcgagccac
    11701 gcagcttgcc gttgacttgc agcatcagtt caatctcgtc ttgcagcaac gcttgcgggt
    11761 ccacctgcgg ccagggtgcg tccagcaaat cgcccaggtg cccggcataa cccagactgg
    11821 accagagcga gtgggtgatg tgcggggtgg ccgggtacag gcagcgcagc aagatgccaa
    11881 aaccttcaat cagcgcgacc t
//
LOCUS       DAOSLL010000061        11867 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5402_length_11867_cov_4.765577, whole genome shotgun sequence.
ACCESSION   DAOSLL010000061 DAOSLL010000000
VERSION     DAOSLL010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11867
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5402_length_11867_cov_4.765577"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="PLB25_04130"
     CDS             complement(<1..260)
                     /locus_tag="PLB25_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE40806.1"
                     /translation="MANQLDLEEQEQLDQIKHFWKQYGNAITWALIVVLAGFASWNFY
                     QYWHRNQAAQAAALFDEVERSIQSADTAKVDRAFAEMKDRFGS"
     gene            complement(273..1559)
                     /gene="hisS"
                     /locus_tag="PLB25_04135"
     CDS             complement(273..1559)
                     /gene="hisS"
                     /locus_tag="PLB25_04135"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HOE40807.1"
                     /translation="MDKLTAVKGMNDLLPPASGAVELLELKVRRLMARYAYRNIRTPI
                     VERTPLFVRGIGEATDIVEKEMYCFEDRLNGDSLTLRPEATAGVVRAALEHSLLYDGP
                     KRLYYMGPMFRHERPQKGRYRQFDQIGAEALGFSGPEVDAELILMATALWQEIGLVDV
                     QLELNSLGQPNERLAHRQALIAYFEQHLSVLDEDARRRLYSNPLRILDTKNPYMQPLV
                     EAAPKLLDFLGKNSQAHFDALRLMLDASGVTYRINPRLVRGMDYYNLTVFEFVTDRLG
                     SQGTVCAGGRYDYLFEQLGGKSTPAVGWALGVDRVLELAKLSGFGQANLVPDAYAVVA
                     EASAMPWVLSTLQVMRTAGLSVQMHPSAPDGMGTMKSQFKRADSSGARFALVFGSDEV
                     SAKQVTIKPLRDAGAVQVTRSLSDLASWAPTLQSAI"
     gene            complement(1598..2869)
                     /gene="ispG"
                     /locus_tag="PLB25_04140"
                     /gene_synonym="gcpE"
     CDS             complement(1598..2869)
                     /gene="ispG"
                     /locus_tag="PLB25_04140"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HOE40808.1"
                     /translation="METSQAVAPACASARNSRQAKVVWGTRIVSVGGSAPVRVQSMTN
                     SDTVDVIGTAIQIKELALAGSEMVRVTVNTPEAAEAVAHIREQLDRMGVDVPLIGDFH
                     YNGHRLLTDFPHCAQALSKYRINPGNVGKGDKHDLQFGLMIEAAMSWNKPIRIGVNWG
                     SLDQELLASLMDQNSHRAKPWNAKSVMYEALISSAIGSARRAVEMGMNPDQIILSCKV
                     SGVQDLISVYRELAKRCDYALHLGLTEAGMGVKGAVASAAAMSILLQEGIGDTIRVSL
                     TPQPGESRTQEVLIATEVLQALEMRAFVPSVTACPGCGRTTSTTFQELTKQIDTYLRL
                     QMPLWRDKYPGVEKMKVAVMGCIVNGPGESKQADIGISLPGTGETPAAPVFIDGEKRM
                     TLRGDSIAWEFQAIVENYVKQRYGAATASNV"
     gene            complement(2869..3810)
                     /locus_tag="PLB25_04145"
     CDS             complement(2869..3810)
                     /locus_tag="PLB25_04145"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HOE40809.1"
                     /translation="MIDADTADSHATVEALAAVPASAGPGAMLRAARESQGLHIAALA
                     VTLKVPVNKLEALESDRFDLLPGTVFTRALAMSVCRTLKMDATPVMAVLPQFQIRQIK
                     TAELGLNASFKSTDGGAKGGWIKQFLNPARLVMVLLLVAIAIIFFWPGKLTAVDAGLV
                     KLNPDSGRDQLGTGSQILSPAMSSTSVVHEPTLLSGPTVREPVRQSASSPALTGSGVI
                     SSSAVVSSPSATETQQSPSVLALDVRGESWVEVTDAQGAAQLRRLALEGETLRVSGAL
                     PLSIVLGRADAVSVSVRGKPFDLSAISKDNVARFEVR"
     gene            complement(3803..4636)
                     /gene="pilW"
                     /locus_tag="PLB25_04150"
     CDS             complement(3803..4636)
                     /gene="pilW"
                     /locus_tag="PLB25_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HOE40810.1"
                     /translation="MNVTANGTWGKHLFFWLAASIFGQLVLPGCASLSDLSGASAGRS
                     ELVTESDESSERKRARIRIELALGYFEQGKTTIALDEIKQAIVADPAFSDAYSLRGLI
                     YMRLNDYATAEESFGKALSINPKNANVLHNLAWLKCQQAEYVPSFALFRRALSEPLYG
                     ERAKTYRAMGLCQERAGMIDEAEASLLRSYEYDAANPVTGYNLANVLYKKKDFVRAQF
                     YIRRLNNSELANAESLWLGIKVERSLSSLAAVAQLATQLEKRFPRAPETSLYRSGAFD
                     D"
     gene            complement(4640..5788)
                     /gene="rlmN"
                     /locus_tag="PLB25_04155"
     CDS             complement(4640..5788)
                     /gene="rlmN"
                     /locus_tag="PLB25_04155"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_043782590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HOE40811.1"
                     /translation="MTVNLLDYDLEGLAVQCERLGEKRFRAIQLFRWIHQKGVRQFDD
                     MTDIANSLREKLKVVACIRPLDVLSQHVSTDGTIKWLFDVGGGNAVETVYIPEAGRGT
                     LCISSQAGCAVGCRFCSTGHQGFSRNLRTGEIIAQLWFAEHFLRQRSGGDDRVISNVV
                     MMGMGEPLHNYDELIPALRTMLDDHGYGLSRRRVTVSTSGVVPMMERLAVDCPVALAV
                     SLHASNDGLRDQLVPLNVKYPLDELLSACVRYLDHAPRDFITFEYCMLEGVNDQPEHA
                     REVVRLVQKHAVDRIWCKFNLIPFNPFPKSGLRCSHNGQIADFARVLSAAGITTTLRK
                     TRGGDIDAACGQLAGDVRDRTKVVERMARQRAVMFGQVGQFDHRTEKV"
     gene            complement(5797..6222)
                     /gene="ndk"
                     /locus_tag="PLB25_04160"
     CDS             complement(5797..6222)
                     /gene="ndk"
                     /locus_tag="PLB25_04160"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HOE40812.1"
                     /translation="MTIERTLSIIKPDAVAKNVIGQIYARFEGAGLKVVAAKMAHLSR
                     GEAEAFYAVHKARPFFKDLVDFMISGPVMIQALEGESAILKNRELMGATDPKKADPGT
                     IRADFADSIDANAVHGSDAAETAAVEIAFFFPGMNVYSR"
     gene            complement(6303..7007)
                     /locus_tag="PLB25_04165"
     CDS             complement(6303..7007)
                     /locus_tag="PLB25_04165"
                     /inference="COORDINATES: protein motif:HMM:NF025596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4338 domain-containing protein"
                     /protein_id="HOE40813.1"
                     /translation="MSLSMVQGTAQPCQWSEYVTRQLYLGYTPMLGAQLPSIGFGGGA
                     WRRKRERFVGWSETHRARNLQLVVNNARLSVLPWTQLKGLASKVLPRVVRQLPQDWLQ
                     RYGNSPMLLATFIESERHKGIWHKATKWINVGGTSGRGKKSLAARRLTPSRTSRPLRY
                     ARPALPSCLSGRDGLTGWLRAMFSAKDDIFDFKQHLSVKETELPSVGNLLAAKFRQKS
                     QPNHHITVNLGPSQQA"
     gene            7382..7612
                     /locus_tag="PLB25_04170"
     CDS             7382..7612
                     /locus_tag="PLB25_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40814.1"
                     /translation="MQLAKQASRNDAVRPNPPVQIGEVISNHILTSIARTVAPKHENF
                     SFKITEHKSIDCALFHANRLEAGGFNLVMDIK"
     gene            7753..8817
                     /gene="fba"
                     /locus_tag="PLB25_04175"
     CDS             7753..8817
                     /gene="fba"
                     /locus_tag="PLB25_04175"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462535.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HOE40815.1"
                     /translation="MALVSMRELLDHAAANGYGIPAYNVNNLEQVQAVMAAADEVGSP
                     VILQASAGARKYAGEAFIKHLIQAAIESYPHIPLVMHQDHGQSVPVCQGAINLGFSSV
                     MMDGSLEADGKTIASYEYNVEVTRKVVDMAHATGVTVEGELGCLGSLETMKGDKEDGH
                     GTDATMTREQMLTNPEQAADFVKRTQLDALAIAIGTSHGAYKFTRKPTGDILAIDRVM
                     EIHARLPNTHLVMHGSSSVPQESLALINQYGGKMKETYGVPVEEIQKAIKYGVRKINI
                     DTDIRLAMTAAVRKYMFENPDKFDARDWLKPAREAAKALCKQRYLEFGCEGQGSKIKG
                     DSLSTVAARYARGELAQVVQ"
     gene            8908..10446
                     /locus_tag="PLB25_04180"
     CDS             8908..10446
                     /locus_tag="PLB25_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HOE40816.1"
                     /translation="MPILAVFIGVTLPWLNPFSPGPTSAAGPFMFAWLCAAGVLLALA
                     FDRQQGQHECMVRAIAMAWLSAAALSALIGLLQYFGATASFGVWLNHTGIGEAYGNLR
                     QRNQFATLLNIGQMALLGLVANAGRSPADWRTRPGQHWKFALGLTLATLLGIGNAASS
                     SRTGLLQLLLLAALVLWWQRGAVRASTRAQLVMLAMLLAYAAASLALPLLAGLDPLNS
                     GAWARLRAGDAACTSRLTLWRNVLHLIELKPWLGWGWGELDYAHFITLYPGERFCDIL
                     DNAHNLPLHLAIELGIPFAVLACGLALLLVWLGRPWREAEPSRQMAWGILAVIGLHSL
                     LEYPLWYGPFQIAALLCIWLLWWLPKSGAPIQIHKSFRPPAQYSKALAAIIITAFVGF
                     ATWNYHLASQIYLAPEARDSAYQSDTLAKIRHIWLFQDQVRFADLTTSELTASNATAL
                     HALALEMLHFSPEARVVEWVLDSALLLGHADEVAFFAERMRAAFPKDYAAWAKANPAA
                     ALPK"
     gene            complement(10501..10902)
                     /locus_tag="PLB25_04185"
     CDS             complement(10501..10902)
                     /locus_tag="PLB25_04185"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOE40817.1"
                     /translation="MLNTASHLCQTRFATIIAALILSMTLHGGAAAADAVQARKDITA
                     MGLEYNGQNFAKAAGNGDLVAVQLFLDAGMDVNEGGGAAIGLAAGRGKTAMVKFLLTK
                     GAKPTSSALQYARTRGYKDIEKILADAGAKE"
     gene            11333..>11867
                     /locus_tag="PLB25_04190"
     CDS             11333..>11867
                     /locus_tag="PLB25_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOE40818.1"
                     /translation="MSSMLIRGGTVINADQAFRADVLTQDGKIVAVGPDLQAPADAIR
                     VDAGGQYVMPGGIDPHTHMQLPFMGTTTMDDFFTGTAAGLAGGTTSIIDFVIPNPQQS
                     LMEAYQTWRGWAEKAAGDYGFHMAITWWDESVKRDMGTLVQQEGINSFKHFMAYKNAI
                     MCDDETLVNSFSRALELG"
BASE COUNT         2700 a         3535 c         3107 g         2525 t
ORIGIN      
        1 gaaccgaaac gatctttcat ttcggcaaaa gctcggtcaa cctttgccgt gtccgctgac
       61 tggatcgaac gctcaacttc gtcaaaaagc gcagccgctt gcgcggcctg gttgcggtgc
      121 caatactggt agaaattcca actggcaaag cctgccagca ccacaatcaa tgcccaagta
      181 atggcattgc cgtattgctt ccagaaatgc ttgatttgat caagctgctc ttgctcttca
      241 agatcgagtt gattcgccat aagttcgctt tgctaaatcg ccgattgtag ggttggtgcc
      301 caggacgcaa ggtcggacag cgaccgcgta acctgaaccg cgccagcatc gcgcaaaggc
      361 ttgatcgtga cctgtttcgc gctgacttca tctgaaccaa aaacaagtgc aaaacgcgcc
      421 ccgctgctat cggcccgctt gaactgactc ttcatggtgc ccattccatc tggcgcgctg
      481 gggtgcatct gcacgctcaa cccggctgtt cgcataacct gcaaagttga caagacccac
      541 ggcatggcgg atgcttcggc cacaaccgcg taagcatctg gcaccaggtt tgcctgtccg
      601 aatcctgata gcttggccaa ctcgagcacc cggtcaaccc ccaaagccca tccgaccgcg
      661 ggcgtggatt tgccgcccaa ctgctcaaac aaatagtcat agcgaccacc ggcacagacc
      721 gtcccctgcg aaccgagacg atcagtgaca aactcaaaca cagtcaggtt gtagtagtcc
      781 atgccacgca ccaacctagg gttaattcgg taagtcaccc cactggcatc cagcatcaat
      841 cgcagagcat caaagtgggc ctgggagttc ttcccgagaa aatcgagcag cttcggtgca
      901 gcttcgacca agggctgcat gtatgggttt ttggtatcca gaatacgcaa ggggttgctg
      961 tagagacgcc gacgcgcgtc ttcgtcaaga actgacaaat gttgctcaaa ataagcaatc
     1021 aaagcttggc gatgtgctag ccgctcgttg ggttgaccaa gactgttcaa ttcaagctga
     1081 acatcaacta ggccaatctc ttgccataac gcagttgcca tcaaaatcaa ttcagcatct
     1141 acttcggggc cagaaaaccc caacgcctcc gcaccgattt ggtcaaattg gcggtaacgc
     1201 cctttttgcg gacgctcgtg ccgaaacatg ggacccatgt agtacaagcg ctttgggccg
     1261 tcgtaaagca gagaatgctc gagcgcggcc cgaacaacgc cagcagtcgc ctcaggacgc
     1321 aaggtcaagg aatcgccgtt tagccgatct tcaaagcaat acatctcctt ttcgacaatg
     1381 tcagtggcct cgccgatgcc tctgacgaac aagggtgtac gctcaacaat gggagtgcgg
     1441 atgttgcggt aagcgtagcg cgccatcaat cgccgcactt tgagctcaag caactccacg
     1501 gcacccgaag caggtggcaa aaggtcgttc atgcctttga cagccgtcaa cttgtcgatt
     1561 ttggaggcaa ctactttgtc ggtcatggtt tcactcttca aacatttgac gctgtcgctg
     1621 cgccgtagcg ctgcttcaca tagttttcga caatcgcctg aaactcccag gcaattgagt
     1681 cgccacgcaa ggtcatacgt ttttcgccgt caatgaagac aggggcggcc ggcgtctcac
     1741 ctgtgcccgg cagactgatg ccaatgtctg cctgcttgct ttcacccggg ccattcacaa
     1801 tacaacccat caccgccacc ttcatttttt caactcctgg atacttgtcc cgccacaatg
     1861 gcatttgcag gcgcaaatag gtgtcaattt gctttgtcaa ttcctgaaag gtggtactgg
     1921 tcgtcctgcc gcaacccgga caagcagtca cacttgggac aaaggcccgc atttcaagcg
     1981 cctgcagtac ctcggtcgca atcagtactt cctgtgtgcg ggactccccc ggctgaggtg
     2041 tcagtgagac acgaatcgtg tcgccaatgc cttcctgcaa cagaatcgac atggctgcgg
     2101 ccgaggcaac cgcccccttg actcccatgc cagcctctgt caggcccagg tgcaaggcgt
     2161 agtcgcagcg ttttgccaac tcgcgataaa cagagatgag atcctggaca ccactgacct
     2221 tgcacgacaa aatgatctgg tctggattca tgcccatctc cactgcccgc cgggcagatc
     2281 cgatcgcaga agatatcaac gcttcataca taaccgactt ggcgttccag ggtttggcgc
     2341 ggtggctgtt ttgatccatg agtgaagcca gtaattcctg atccaggcta ccccagttga
     2401 caccaatgcg aatcggcttg ttccagctca tcgcagcttc aatcatcaaa ccgaactgca
     2461 agtcgtgctt gtcacctttg ccaacattcc ccgggttgat ccggtacttg gacagtgctt
     2521 gtgcgcagtg gggaaaatcg gtcaacaacc ggtgaccgtt gtagtggaaa tcgccgatca
     2581 ggggcacatc cactcccatg cggtcaagct gttcgcgaat gtgcgccaca gcctcggccg
     2641 cctcgggtgt gttgacagtc acccgaacca tctcggagcc ggccagagcg agctctttga
     2701 tctgaatggc ggtaccaatc acgtcaaccg tgtcagagtt ggtcatcgac tgaacacgca
     2761 ccggcgcact gcctcccaca gagacgattc gcgtccccca aaccaccttc gcctgtcgcg
     2821 aatttcttgc cgacgcacaa gccggggcca ctgcctgtga tgtctccatt accgcacctc
     2881 aaatcgcgca acgttgtctt tggaaatcgc tgacaggtca aacggctttc cgcgaaccga
     2941 tactgatact gcatcggcac ggcccagaac aatagacagt ggcagcgcac cagacacgcg
     3001 caaggtttca ccctccaatg cgagtctgcg cagttgtgcc gcgccttgcg catcggtcac
     3061 ctctacccag gattccccgc gaacgtccag cgccagtacc gaaggtgatt gctgtgtctc
     3121 tgttgcgctt ggcgaactga ccaccgcgct agaagaaatc acaccgcttc cagtcaacgc
     3181 cggtgagctt gccgattgcc ggactggttc ccgaaccgtt ggtccagaaa gaagcgttgg
     3241 ctcatgcaca acggatgtcg aactcatcgc aggtgacaaa atctgtgatc cagtgcccaa
     3301 ttgatctcgc cctgaatcgg gattgagctt gacaagaccc gcatcaacgg ccgtaagctt
     3361 gccgggccag aaaaaaataa tggctattgc caccaacaac aacaccatca ccaggcgcgc
     3421 cggattcaaa aactgcttga tccagccacc tttcgcgccg ccatccgtcg atttgaagga
     3481 cgcattcaga cccaactcag ctgtcttgat ctgccggatt tggaactgtg gcaacacagc
     3541 catgaccggt gtcgcgtcca ttttcaaagt acgacaaacg ctcatcgcaa gcgctctggt
     3601 aaaaaccgta ccgggaagaa gatcaaatct gtcagactcc aacgcttcca gtttattgac
     3661 tggaaccttt agggtcaccg ccagggcagc aatgtgcagg ccctgcgatt cccgtgcggc
     3721 gcgcaacatg gcaccaggcc cggcgcttgc aggaactgca gcaagcgctt ccacggtcgc
     3781 atggctgtcg gctgtgtcag catcaatcat caaaagctcc actacgatac aaggatgttt
     3841 cgggagcccg aggaaagcgc ttttctaact gggtggcaag ttgtgctacc gcagccagac
     3901 tggacaggct tctttcaacc ttgattccca accaaagtga ctcggcattc gcaagttcgc
     3961 tattgtttaa ccgacggata taaaactgag ctcgcacaaa gtccttcttc ttgtaaagca
     4021 cgttggcgag gttgtatcca gttactgggt tagcagcgtc gtattcatag gatctgagca
     4081 agcttgcttc ggcttcgtca atcatgccgg cacgctcctg gcacaggccc atggctctgt
     4141 aagtcttggc tctttcgcca tacagcggct cagacagtgc ccgacgaaaa agggcaaacg
     4201 acggcacata ctcggcttgc tggcatttaa gccaagccag attgtgcagt acattggcat
     4261 tcttgggatt gatcgacaag gccttgccaa agctctcctc cgcagtagca tagtcattca
     4321 agcgcatgta gataaggcca cgcagactgt aggcatccga aaaagccggg tcggcaacaa
     4381 tggcctgctt gatttcgtcc agcgcgatag tcgtctttcc ttgttcaaaa taaccaagcg
     4441 cgagctcaat ccgaatacgt gcccgcttgc gctcgcttga ctcatcggat tctgtaacca
     4501 attcgctgcg ccctgcgctt gcaccagaca gatctgacag gcttgcacaa cccggcagca
     4561 ccagttgtcc aaaaattgac gcggcaagcc aaaagaaaag atgcttaccc caagttccat
     4621 tcgcggtcac gttcatcact cagacctttt ctgtcctgtg gtcgaactga cccacttgcc
     4681 caaacatcac cgctcgttgc cgagccattc gctcgaccac cttggttcga tcccggacat
     4741 cgccagccaa ttgaccgcaa gcggcatcaa tgtcaccgcc acgtgtcttt cgcagtgtgg
     4801 tggtgatgcc agcagcactg agaactctcg caaaatcagc aatctgtcca ttatgggagc
     4861 atcgcaagcc agatttagga aaaggattga agggtatcaa attgaatttg caccagattc
     4921 tgtcaactgc gtgtttttga accaagcgaa ccacttcgcg agcatgctca ggctggtcat
     4981 tcacaccctc aagcatgcag tattcaaagg taatgaaatc acgcggtgca tgatcaaggt
     5041 aacggacaca ggctgaaagc aactcatcaa gtggatactt gacgttcagg ggaaccaact
     5101 ggtcacgcag accatcgtta gatgcgtgca atgacacagc caatgcaact ggacaatcga
     5161 ccgccagacg ttccatcatc ggaacaacgc ctgaagtgga cacggtcaca cgccgcctcg
     5221 acagcccata gccatgatcg tcgagcatcg ttctcaatgc cggaatgagc tcatcatagt
     5281 tatgcaacgg ttctcccatg cccatcatga ccacattgga gataacccga tcatcccccc
     5341 ccgaacgctg gcgcaaaaaa tgttcggcga accaaagctg cgcaatgatt tctccggtcc
     5401 gcaggtttcg gctgaagcct tgatgaccag tcgaacaaaa ccgacacccg acggcacacc
     5461 cagcctgaga agatatacat aaggtaccgc gccccgcctc tggaatgtac acagtctcaa
     5521 ccgcgttgcc gccgccaaca tcaaacaacc atttgattgt gccgtcagtc gaaacgtgtt
     5581 gcgacaagac atccagtggt cttatacagg ctacaacttt gagtttttca cgcagagaat
     5641 tggcaatgtc cgtcatatca tcaaattgac gaaccccctt ctgatgaatc caacgaaaca
     5701 actgaatcgc acggaaacgc ttttcaccca gccgctcgca ttggacggcc aagccctcga
     5761 ggtcatagtc gagcaggttg accgtcatac aaatgcttaa cgagagtaaa cgttcatgcc
     5821 agggaaaaag aaggcaatct ctactgcggc tgtttcggca gcatcagagc catgaacagc
     5881 attggcatca atactgtcgg caaaatcagc acgaatggtg ccgggatctg ccttctttgg
     5941 atcagttgca cccatcaatt cgcgattttt caaaatcgcg ctctcccctt cgagggcctg
     6001 aatcatgacg gggccggaga tcatgaaatc taccaagtcc ttgaaaaaag gccgcgcctt
     6061 gtgcactgcg taaaaagcct cggcctcgcc gcgtgacaaa tgtgccattt tggcagcgac
     6121 caccttcaag ccagcgcctt cgaagcgtgc atagatttgc ccgatcacgt ttttcgcaac
     6181 agcgtctggc ttgatgatgg agagtgttct ttcgatagtc atgttttttc cttagacaaa
     6241 attggacttt ggacaccgcg gccaaagctt cttattttag cctgaacgtt tttccgctga
     6301 actcacgctt gctgactcgg gccgagattc accgtgatgt ggtgatttgg ctggcttttt
     6361 tgcctgaact tcgctgccaa caggttgccc actgacggca gttccgtttc cttcactgac
     6421 aaatgttgtt tgaaatcaaa gatgtcgtcc ttcgcactga acatcgcgcg taaccacccg
     6481 gtcaatccat cccgtccact caagcaagaa ggcagcgcag gtcttgcgta gcgcaaaggt
     6541 cgcgatgtcc ttgatggggt caagcgcctt gctgccagtg acttcttgcc gcgcccactg
     6601 gtaccaccga cattgatcca tttggtggcc ttgtgccaaa tgcctttgtg gcgctcagat
     6661 tcgataaagg tcgccagcag cattggcgag ttgccgtaac gctgcaacca atcctgcggc
     6721 aattgacgta ccacgcgagg gaggactttc gaggccaggc ctttgagctg cgtccaaggg
     6781 agaacagaca agcgtgcatt gttgaccacc aattgcaaat tgcgcgccct gtgcgtttcg
     6841 ctccagccta caaagcgctc acgcttgcgt ctccaagcac ctccgccaaa accgatggac
     6901 ggaagctgag cacccaacat cggtgtgtag cccaaataga gctgccgcgt gacatattcg
     6961 ctccactggc acggttgggc cgtaccctga accatactca gcgacaagtg gccaggctca
     7021 tggaccgatt acccaatcgc agtttcggtg tcgcttgcgc cggtggttgg gaagtgaggt
     7081 cgccccagcg cccggcaaga aggcgtgcag gcggcagcac aatcaagcca tcacttgcat
     7141 ccaggcacca gcgctacccg gcaggtcatg tccttgagcg ccccatcggg ttgtcattgc
     7201 gggcacgaac tgcaattcac cacctccgga cttcctggtt gccgggtcgc gccgggcatg
     7261 acagacccgc gttcatcgcg acacgcagac attttcatag cggaagctaa ggatgtggtc
     7321 gggaataaca ccccgttttg caccaaggag ttgggatgca cggctaggcg cgagacgcgc
     7381 cgtgcaactg gctaaacaag cgtctcgcaa cgacgctgtg cgcccaaatc cgccggtgca
     7441 aataggggaa gttatttcta accacatcct aacttccatc gccaggacgg tcgccccgaa
     7501 acatgaaaac ttttcattca aaattactga acataaatca atcgattgcg cgttgtttca
     7561 cgccaaccgg ctggaagccg gtggatttaa ccttgtgatg gacattaaat aaatcaattt
     7621 gatttgataa attgttgaca gactgaaggc ggttttcggt gccagggctg gtgctggctt
     7681 gaatttcgtg ctacttcaat cgctaaaata atctgaattt cgcaactatt accaaccaga
     7741 aaggacacca tcatggctct tgtttccatg cgcgaattgc ttgaccacgc tgccgccaac
     7801 ggctacggca taccggccta caacgttaac aaccttgagc aagttcaggc tgtcatggca
     7861 gccgccgatg aagtcggctc ccctgtcatc ctgcaagcca gtgccggggc tcgcaaatac
     7921 gctggcgaag ccttcatcaa gcacctgatc caggccgcca ttgaatccta tccgcacatt
     7981 ccgctggtga tgcaccagga tcatggtcaa agcgtgccgg tgtgccaggg tgccatcaac
     8041 ctgggcttta gctcggtgat gatggacggc agtctggaag ccgacggtaa aaccattgcc
     8101 agctacgaat acaacgtgga agtgacccgc aaagtggtcg atatggcgca cgccaccggt
     8161 gtcacggtgg aaggtgaact cggctgcctg ggcagtctgg aaaccatgaa aggcgacaag
     8221 gaagacggtc atggcacaga tgccaccatg acgcgtgaac agatgctgac caaccccgaa
     8281 caggctgccg actttgtcaa gcgcacccag ctcgacgcat tggcgattgc cattggcacc
     8341 agccacggtg cctacaagtt cacccgcaag cccaccggcg acatcctggc aattgaccgc
     8401 gtgatggaaa tccacgctcg cctgcccaac acccatctgg tgatgcatgg ctcaagcagt
     8461 gtgccgcaag agtcgctggc gttgatcaac cagtacggcg gcaagatgaa agagacctac
     8521 ggtgtgccgg tcgaagagat ccagaaagcc atcaagtacg gcgtgcgcaa gatcaacatc
     8581 gacaccgaca ttcgcttggc catgaccgct gcggtgcgca agtacatgtt tgaaaaccct
     8641 gacaagtttg acgcgcgcga ctggctcaag ccggcccggg aagccgccaa agccctgtgc
     8701 aagcagcgct accttgaatt cggctgcgaa ggccagggca gcaaaatcaa gggtgacagc
     8761 ctgagcacgg tggctgcccg ttacgcccgt ggcgaactgg cgcaagtcgt tcagtaacgc
     8821 ctaaccccgg caccgcaacc cgccactggc gctggccaat ggcgggtttt tttgcgacct
     8881 gccccaccgg atctgactca agcacacatg cccatccttg ccgttttcat tggtgtcacc
     8941 cttccgtggc tgaacccgtt ctcgcccggc ccaaccagcg cagccggacc cttcatgttt
     9001 gcctggctgt gtgccgccgg ggtcctgctg gccctggctt ttgaccggca gcaggggcag
     9061 catgaatgca tggtgcgtgc cattgcaatg gcctggctga gcgccgcagc cctcagcgcg
     9121 ctgattggac tgctgcagta ctttggcgca acagccagtt ttggcgtctg gctgaaccac
     9181 accggcatcg gtgaagccta tggcaatctg cgccagcgaa accagtttgc caccctgctc
     9241 aacattggac agatggcttt gctggggctt gtcgcaaacg ccggacgcag tccggcagac
     9301 tggcgcaccc gccctggcca gcactggaag ttcgcccttg gactgaccct ggctaccctg
     9361 ttgggcatcg gcaatgccgc atcatcgtcg cgcaccggcc tgctgcaact gctgctgctg
     9421 gcggctctgg tgctgtggtg gcagcgcggc gctgtacgcg cgtcgacgcg ggcccagttg
     9481 gtcatgctgg cgatgctgct ggcctacgcg gcagccagcc ttgcattgcc actgctggcc
     9541 gggcttgatc ccctgaacag cggtgcctgg gcgcgcctgc gtgccggtga cgcagcctgc
     9601 accagtcgcc tgacgctgtg gcgcaatgtg ctgcacctga ttgaactcaa gccctggctg
     9661 ggctggggtt ggggcgaact ggactacgcg cacttcatta ccctgtatcc gggcgagcgc
     9721 ttttgcgaca tcctggacaa cgcccacaac ctgccgctgc atctggccat tgagcttggc
     9781 atccctttcg cagtgctggc ttgcgggctg gctttgctgc tggtctggct tggtcggccc
     9841 tggcgcgaag cagagcccag ccgtcaaatg gcctggggca ttctggcagt catcgggctg
     9901 cacagcctgc tcgaataccc gttgtggtac ggcccgtttc aaatagcggc gctgctgtgc
     9961 atctggctac tgtggtggct gccaaagtct ggcgcaccca tccaaattca taagtctttt
    10021 aggcctccag cccagtacag caaagcgcta gcagctatca ttattacagc gtttgttggt
    10081 ttcgccacct ggaactatca cctggccagc cagatttacc tggcacccga ggcgcgtgac
    10141 agcgcgtacc agagcgacac gctggcaaaa attcgccata tctggctgtt tcaggaccag
    10201 gtgcgctttg ctgacctgac cacgtccgag ttaactgcca gcaacgccac ggcgcttcac
    10261 gcgctggcgc tggaaatgct gcatttttcg ccagaagcac gggtcgtcga atgggtgctc
    10321 gacagcgctt tgttgttggg ccacgccgac gaagtggcgt tttttgcaga acgcatgcgc
    10381 gccgccttcc caaaagatta cgcggcctgg gcaaaagcca atccggctgc cgcgctaccc
    10441 aaatgacggc gacgcggcaa cggtgagcgg gctagccacg gcggcctgca cctgtgttgc
    10501 ctattccttg gcgcctgcat ccgcgagtat cttttcgatg tctttatagc ctcgcgtcct
    10561 ggcatattgc aacgcgctag aagttggctt ggcgcctttg gtcagcagaa acttcaccat
    10621 cgcagtcttg cccctgccag cagccaggcc aatggcagca cccccgccct cattgacatc
    10681 catacccgca tccaggaaaa gctgcaccgc caccagatcg ccattgccag cagccttggc
    10741 aaagttctgc ccgttatatt ccagccccat cgccgtgatg tccttgcgcg cctgcaccgc
    10801 atctgccgcc gctgcaccac cgtgcaatgt catggaaagg atgagtgcgg caatgatggt
    10861 tgcaaagcgc gtctggcaaa ggtgtgaagc agtgttgagc acatcaatct cctcgataca
    10921 aaatcatgaa ttcgcaaaat cactctagca tatccaaagg ccctgcacgg cgcgcgcgga
    10981 cagtggctgc gtttgaatca tttgatcatc cgtgagcaat gcgcaggtgt cactttggct
    11041 acgacattga accggttgcg gcggtcattg gccggtgcga gcggcgcgga tgccagggtt
    11101 gcatcagcca ctgatcaacc gcgcgtgcag attggagcga tcacgattgc gctggacttt
    11161 attttccacc tcgtggcaac atggtggcat cgtcataatg tcaatgcatc ccgtgtttca
    11221 gcggtcgcca gcacccggct ggcaacacgc caaacgattc ccatttgtat gacaaatcag
    11281 cttgcagccc ttatctggca agcgatgcaa gctatttatt atggagtaaa tcatgagcag
    11341 tatgttgatt cgaggcggca cagtcatcaa cgccgaccag gccttcaggg ccgatgtgct
    11401 gacgcaggac ggcaaaattg tggcagttgg ccctgacttg caagccccgg ccgatgccat
    11461 acgggtcgat gcggggggcc aatatgtgat gccaggcggc attgacccgc acacccacat
    11521 gcagttgccg ttcatgggca ccaccaccat ggacgacttt tttaccggca ctgcagcagg
    11581 cctggcgggc ggcaccacca gcatcatcga ttttgtcatc ccgaacccgc agcaaagcct
    11641 gatggaggcc taccagacct ggcgcggctg ggccgaaaag gcagcgggcg actatggctt
    11701 tcacatggcc atcacctggt gggacgagtc ggtcaagcgt gacatgggca cgctggtgca
    11761 gcaggagggc atcaacagct tcaagcattt catggcctac aagaacgcca tcatgtgtga
    11821 cgacgaaaca ctggtgaaca gcttcagccg cgcgctggaa ctcggtg
//
LOCUS       DAOSLL010000062        11622 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5523_length_11622_cov_5.487767, whole genome shotgun sequence.
ACCESSION   DAOSLL010000062 DAOSLL010000000
VERSION     DAOSLL010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11622
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5523_length_11622_cov_5.487767"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="PLB25_04195"
     CDS             <1..694
                     /locus_tag="PLB25_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOE40819.1"
                     /translation="SQACLMMDADPAVRVLVLAGRGRHFSAGADLNWMRRAGAASEAD
                     NLSDARRFARMLRSLSGLSKPTIARVQGAALGGGTGLAAACDMAVASSDAVFSTSEVR
                     FGIIPAVISPYVLRAIGPRHALRYFQTGEKISAERALMIGLVSDVTPVDALDAGVAAL
                     IEPLLAGAPSAQAASKELIEPIKGRLIDDAIMEETARRIARQRATAEGRDGVAAFLDK
                     RPPAWLVDQPGT"
     gene            714..1727
                     /gene="paaA"
                     /locus_tag="PLB25_04200"
     CDS             714..1727
                     /gene="paaA"
                     /locus_tag="PLB25_04200"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006053057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HOE40820.1"
                     /translation="MYTQSLNVQELPGQNRAAPVVSLESPELQARFDRKLKADGKIEP
                     QDWMPAGYRKTLIRQISQHAHSEIVGMLPEGNWISRAPSLKRKAILIAKVQDEGGHGL
                     YLYSAAETLGVGRDQMLADLHSGRAKYSSIFNYPTLNWADVGVIGWLVDGAAISNQIP
                     LCRCSYGPYARAMIRVCKEESFHQRQGYEALLVMMQGTAAQKAMVQDAVNRFWWKCLA
                     MFGPPDANSPHSEQAFAWGIKRISNDELRQKFIDATVPQAQVLGITLPDPKLKWNEQR
                     QHYDHGEIDWDEFWATINGNGPCNKERLATRVKAHNDGQWVRDAALAYAQKQQAKQQK
                     EAA"
     gene            1724..2062
                     /gene="paaB"
                     /locus_tag="PLB25_04205"
     CDS             1724..2062
                     /gene="paaB"
                     /locus_tag="PLB25_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HOE40821.1"
                     /translation="MSTPNPQPDPSPKATQPPPLKDWPLWEVFVRSKQGLEHKHCGSL
                     HASDARHALQMARDVYTRRQEGVSIWVVPSNSITASNPDDKDALFEPAADKIYRHPTF
                     YDIPDEVGHM"
     gene            2062..2850
                     /gene="paaC"
                     /locus_tag="PLB25_04210"
     CDS             2062..2850
                     /gene="paaC"
                     /locus_tag="PLB25_04210"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020423959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HOE40822.1"
                     /translation="MASHYQDYLLHLADNALILGQRNAQWCGHGPVLEEDLALANNSL
                     DLIGQARLLYQHVVTVRRDGKSSEDLLAYFRDVPDFKNYTLLELPHSTALAPTAAAER
                     DYAVTIARNFLYSALMVLVWDGLKSSGDAQLAAIAAKSLKETSYHLRHAHDWLLRLGD
                     GTAESHARMQAAVDHLLPYTQEFFAPSAMETAVIKAGLGVATQPLQAAWQAMVDEALA
                     QATLTRPAVAGHVSTGKIGVHSEHLGFVLAEMQSLARAHPEAVW"
     gene            2856..3416
                     /gene="paaJ"
                     /locus_tag="PLB25_04215"
     CDS             2856..3416
                     /gene="paaJ"
                     /locus_tag="PLB25_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaJ"
                     /protein_id="HOE40823.1"
                     /translation="MTADLPIGPIARKLPSDRHFAIWNRLESVTDPEIPVVNLREMGI
                     LRAVRQSSGALEVVITPTYSGCPALGQMTDDVVQALAQAGVAARVLTQIAPAWSTDWI
                     SAEGCEKLRRYGIAPPLPGAGASGALKAIQFAAKSIAGSAMLTVACPQCGSHNTTETS
                     HFGSTACKALYRCLNCLEPFDYFKPY"
     gene            3440..4543
                     /locus_tag="PLB25_04220"
     CDS             3440..4543
                     /locus_tag="PLB25_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HOE40824.1"
                     /translation="MSQAPRFHDLRVSGISPEAAGSVAVTFAIPESLLECFDFKPGQY
                     LTLRSKIDGADVRRSYSICCTHSHLQARHELVVGIRPMAGGVFSNWAASQLKVGDTLS
                     VLPPEGRFTSRRPRALHRVGFAAGSGITPILSIMASSLEDQPNSRFTLVYGNRSMATV
                     MFNEALQDLKDRFASRLTLIHILSRQAQEVDLLQGRIDGDKVRALISTLLPVASMDEV
                     FVCGPHAMIEATEKALLEAGVPSRQVHTERFTSPTLESLSPAERQAASGQLNLPAGGQ
                     VALTVVLDGKAHALKMNPDEHVLDAALAAGLDLPFSCKGGVCCTCRVKVLQGSVAMDK
                     NFGLEAWETDQGFVLSCQSRPTSQAVTVSYDER"
     gene            complement(5287..6192)
                     /locus_tag="PLB25_04225"
     CDS             complement(5287..6192)
                     /locus_tag="PLB25_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE40825.1"
                     /translation="MIEMLGATDQFVMELPWLAGLMHRLFGTSVKPCADHQLKAQSPV
                     RFSHTPFGTRSYYLAGSGSPTVIFESGLGDGKEVWAPVFNALSRKTRVLAYDRAGYGQ
                     SDKSSWTRDGFQIVHELRALLRTENIAPPYVLVGHSLGGTLVKLFARTYPDEVAGVVL
                     VDARHADFVGRCRKMGLLRLLYEPPLPLFLLGRSAMRSELLAAPATMEQARSTGTFPN
                     VPLRVLTHRRRFLYQTRGLALAWADCQSSLAGMSAQGLLRICDQSGHHVHRDRPDLVI
                     RAILSVMASLPLPFELADPHRLMPS"
     gene            complement(6763..6987)
                     /locus_tag="PLB25_04230"
     CDS             complement(6763..6987)
                     /locus_tag="PLB25_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40826.1"
                     /translation="MRHKPDKLPARFWVAAFAGMIELHGNKLFLAGRNVTCIAPNGLF
                     RPNNTCPALLVGAPILFGIAGVAAGLYQSA"
     gene            complement(7077..8789)
                     /gene="yidC"
                     /locus_tag="PLB25_04235"
     CDS             complement(7077..8789)
                     /gene="yidC"
                     /locus_tag="PLB25_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HOE40827.1"
                     /translation="MNDIRRTILWVIFGFSMVMLWDKWQIHNGNKPTFFPGPAPTVAQ
                     APAPSKTASGVDSVPVATTNVAVGARPTGATAELVPQVSSSVAHQQVVAETDVLKVTF
                     DTEGGSVVRTEFLKHMDMADKARQFVLLDDSKDRVYLAQSGLIALAGGAALPNHKSVM
                     TLVPGERVLKDGSQSLELRFESPEVGGVKLVKTYTLARGSYAVAVKHEVVNLGAAAVA
                     PQLYLQLVRDGNKPPGESSFYSTFTGPAVYTDAKKYQKVEFADIEKNKIDIEKTADNG
                     YVAMVQHYFASAWLLGDGIKRDLFMRRVDNNLYAVGMIAPLEAIAPGARKSVEARFFA
                     GPQEESVLESMAPGLELVKDYGWLTILAKPLYWLLVKLHALIGNWGWSIMALVLLLKA
                     AFYWLNAKAYASMAKMKAVNPKITEMRDRLKDNPQQMQQEMMRIYREEKVNPMGGCFP
                     IMIQIPVFIALYWVLLSSVEMRNTPWLGWIHDLSSPDPYYILPLVMTLTTVLQTSLNP
                     APPDPMQAKMMWFMPLAFSVMFFFFPSGLVLYWITNNVLSIAQQWVINTRMGVPPKFH
                     LPKF"
     gene            complement(8786..9070)
                     /gene="yidD"
                     /locus_tag="PLB25_04240"
     CDS             complement(8786..9070)
                     /gene="yidD"
                     /locus_tag="PLB25_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HOE40828.1"
                     /translation="MIARFLIRLVKAYRLLLSPSLGSSCRFEPTCSAFALQALEQHGA
                     LNGSYLTLGRIGRCHPWCDGGFDPVPGAAPLARSGLLTRLISSTTQKKSS"
     gene            complement(9084..9506)
                     /locus_tag="PLB25_04245"
     CDS             complement(9084..9506)
                     /locus_tag="PLB25_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HOE40829.1"
                     /translation="MQRLKTRPQFQAVLAGVTLVRTPHFALHRVALSGSTPSAPDASP
                     LFAKRDVWLGAMVPKRWAKRAVTRNSIKRLVYAVSLDFEANLACAAHVVRLRSSFDRQ
                     HFVSATSLALKIAVRQELQQLFSKAATAPPLTVQPGVA"
     gene            complement(9530..9664)
                     /gene="rpmH"
                     /locus_tag="PLB25_04250"
     CDS             complement(9530..9664)
                     /gene="rpmH"
                     /locus_tag="PLB25_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HOE40830.1"
                     /translation="MKRTYQPSKIRRARTHGFLVRMKTRGGRAVINARRAKGRKRLAV
                     "
     gene            complement(10461..10709)
                     /locus_tag="PLB25_04255"
     CDS             complement(10461..10709)
                     /locus_tag="PLB25_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40831.1"
                     /translation="MKVPSRHWPKPQTPGGGTNALACAAGSARCGAFRFANITDVQCG
                     YKGLPHGQNRERAGKWAARLTAWLLGVTCGLRWAEVAP"
     gene            complement(10741..10830)
                     /locus_tag="PLB25_04260"
                     /pseudo
     CDS             complement(10741..10830)
                     /locus_tag="PLB25_04260"
                     /inference="COORDINATES: protein motif:HMM:NF015164.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(11367..>11622)
                     /locus_tag="PLB25_04265"
     CDS             complement(11367..>11622)
                     /locus_tag="PLB25_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40832.1"
                     /translation="PDIIIGSWCGKKFRPENVAARPGWSEVAAVKTGQLFEIKSPDIL
                     QPGPAALTDGVAQLHRIIMAWCKNVASKSATSVAQGDIEG"
BASE COUNT         2470 a         3539 c         3454 g         2159 t
ORIGIN      
        1 gagccaagcc tgcctgatga tggatgccga ccctgctgtg cgggtgctgg tgctggccgg
       61 gcgcggccgg catttttctg ccggggctga cctgaactgg atgcgccgtg ccggcgccgc
      121 cagcgaagcc gacaacctgt ctgatgcacg gcgctttgcg cgcatgctgc ggtccttgtc
      181 cggcctgtcc aaacccacca ttgcccgcgt ccagggtgcc gcgctggggg gcggaaccgg
      241 gctggcggcg gcctgcgaca tggcggtggc cagcagtgac gcggtgtttt ccacctccga
      301 agtcaggttt ggcatcatcc cggcggtcat cagcccctat gtgctgcggg cgattgggcc
      361 gcgtcatgcg ctgcgctact ttcagactgg cgaaaaaatc agcgccgagc gggccttgat
      421 gatcggtctg gtcagcgacg tcacgccggt ggacgcgctc gatgccggtg tggccgcctt
      481 gatcgaaccc ttgctggctg gtgcgccatc ggcacaagcg gccagcaagg aactgatcga
      541 gcccatcaag ggccgcttga tcgacgatgc catcatggaa gaaaccgccc ggcgcatcgc
      601 ccgccagcgc gccaccgccg aaggccgcga cggtgtggcc gcgtttctgg acaagcggcc
      661 accggcctgg ctggtggacc agcccggcac ttaacccaca ggagtcaatg ctgatgtaca
      721 cccagtcgct caacgtgcaa gagctacccg gccagaacag ggccgcaccg gtagtttcgc
      781 tggaaagccc cgagctgcag gcccgctttg accgcaagct gaaggccgat ggcaagatcg
      841 agccgcagga ctggatgccg gcaggctatc gcaagaccct gatccgccag atcagccagc
      901 acgcgcacag cgaaattgtc ggcatgctgc ccgagggcaa ctggatcagc cgcgctccca
      961 gcctgaaacg caaggcgatt ctgatcgcca aggtgcagga cgaaggtggt cacggcttgt
     1021 acctctacag cgcggctgaa accctggggg ttggccgcga ccagatgctg gccgacctgc
     1081 acagcggtcg cgccaaatac agctcgatct tcaactaccc gacgctcaac tgggctgatg
     1141 ttggcgtgat tggctggctg gtggatggtg ccgccatcag caaccagatt ccactgtgcc
     1201 gctgcagtta tggcccttac gcgcgcgcca tgatccgcgt ctgcaaggaa gagtctttcc
     1261 atcagcgcca gggttatgag gcgctgctgg tcatgatgca gggcaccgcc gcgcaaaagg
     1321 ccatggtgca ggacgcggtc aaccgcttct ggtggaaatg cctggccatg tttggcccgc
     1381 ccgatgccaa cagcccgcat agcgaacagg cttttgcctg gggcatcaag cgcatcagca
     1441 atgacgagct gcgccagaag ttcatcgatg ccaccgtgcc acaggctcag gttctgggca
     1501 tcacgctgcc tgacccgaaa ctgaaatgga acgagcagcg ccagcattac gaccatggcg
     1561 aaatcgactg ggacgagttc tgggccacca tcaacggcaa cggcccgtgc aacaaggagc
     1621 gcctggcaac ccgcgtcaaa gcccacaacg acggccagtg ggtgcgcgat gccgcgctgg
     1681 cctatgccca gaagcaacag gcaaagcaac aaaaggaagc cgcatgagca cgccaaaccc
     1741 gcaacccgac ccaagcccga aggcaactca gcccccaccg ttaaaagact ggccactttg
     1801 ggaggttttt gtgcgcagca aacaaggcct ggagcacaag cactgcggca gcctgcatgc
     1861 gtccgatgcc cggcacgcgc tgcaaatggc gcgcgatgtc tatacccgcc gccaggaagg
     1921 tgtcagcatc tgggtggtgc cgtccaacag catcaccgcc agcaacccgg acgacaagga
     1981 cgcgctgttt gagcccgccg ccgacaaaat ctaccgccac cccaccttct atgacattcc
     2041 ggatgaagtg gggcacatgt aatggcaagc cactaccagg actacctgct gcacctggcc
     2101 gacaacgcgc tgattctggg ccagcgcaat gcccagtggt gcggccatgg cccggtgctg
     2161 gaagaagatc tggcgctggc caacaacagc ctggacctga tcgggcaagc ccggctgctg
     2221 taccagcatg ttgtgacggt gcgccgcgac ggcaaaagca gcgaagacct gctggcctac
     2281 tttcgcgatg tgccggactt caaaaactac accttgctgg aactgccgca cagcaccgcg
     2341 ctggcaccca cggctgcggc cgagcgcgac tacgcggtaa ccatcgcccg caactttctc
     2401 tacagcgcgc tgatggtgct ggtgtgggac ggtctgaaaa gttcaggcga tgcgcaactg
     2461 gccgccatcg cggccaaatc gctgaaagaa accagctacc acctgcgcca cgcacacgac
     2521 tggctgctgc gcctgggcga tggcaccgca gagtcgcatg ccaggatgca ggcggcggtc
     2581 gatcatttac tgccctacac gcaggagttc tttgccccga gcgccatgga aaccgccgtc
     2641 atcaaggctg gcctgggggt tgccactcag ccactgcaag ccgcctggca ggcgatggtg
     2701 gacgaggcac tggcgcaagc gaccttgacg cgcccggcag ttgccgggca tgtgtccacc
     2761 ggcaaaattg gcgtgcattc cgagcacctg ggttttgtgc tggccgagat gcaaagcctg
     2821 gcgcgcgccc accccgaggc ggtctggtaa gcaacatgac agcggacctg ccgattgggc
     2881 cgatagcgcg caagctgccg tctgaccggc actttgccat ctggaatagg ctggagtcgg
     2941 tgaccgaccc cgagattccg gtggtcaacc tgcgcgagat gggcattttg cgggccgtgc
     3001 gccaatcctc tggcgcgctt gaggtggtca tcaccccgac ttacagcggc tgcccggccc
     3061 tgggccagat gaccgacgac gtggtgcagg ccctggcgca ggccggcgtg gcggcccggg
     3121 tgctgacgca aatcgccccg gcctggtcca ccgactggat cagcgctgaa ggctgcgaaa
     3181 agctgcgccg ctacggcatt gccccgccgc tgcccggtgc tggtgcgtcg ggggcgttga
     3241 aggccatcca gtttgctgca aaatctatag ctggcagcgc aatgctgacg gtggcttgcc
     3301 cccaatgtgg ctcacacaac acgaccgaga cctcacactt tggctcgacc gcttgcaagg
     3361 cgctgtaccg ttgcctgaat tgcctcgaac cctttgatta cttcaaaccc tattaagacg
     3421 gcgaccgcgc tgccccaaca tgtcccaagc cccccgtttt cacgacctgc gcgtgtctgg
     3481 catcagccca gaggcggccg ggtcggtggc cgtcaccttt gccattccag agtcgctgct
     3541 ggagtgcttt gacttcaagc ctggccagta tctgacgcta cgcagcaaga ttgacggtgc
     3601 cgatgtgcgg cgcagttatt cgatttgctg cacccacagc caccttcaag ccaggcatga
     3661 actggtggtc ggtatccggc cgatggcggg cggcgtgttt tcgaactggg ccgccagcca
     3721 gctcaaggtc ggcgacacgc tgtcggtgct gccgcccgaa ggccgcttca cctcgcggcg
     3781 accacgcgcc ttgcaccgcg tgggttttgc cgcgggctcg ggcatcacgc cgattttgtc
     3841 gatcatggcc agcagcctgg aagaccagcc aaactccaga ttcaccctgg tctatggcaa
     3901 ccgcagcatg gccacggtga tgttcaacga ggccttgcaa gacctgaaag accgctttgc
     3961 cagccgcctg acgctgatcc acatcctgtc gcgccaggcg caagaggtgg acttgctgca
     4021 agggcgcatc gatggcgaca aggtgcgcgc cctaatcagc acgctgttgc cggtggccag
     4081 catggacgag gtgtttgtct gcggcccgca cgccatgata gaagccaccg aaaaagccct
     4141 gctggaggcc ggtgtgccgt caagacaggt gcacaccgag cgctttacct cacccacgct
     4201 ggaatccctg agccccgccg agcgccaggc tgccagcggg cagcttaacc tgcccgctgg
     4261 cggacaagtg gccttgaccg tggtgctgga tggcaaggcc cacgcgctaa aaatgaaccc
     4321 tgatgaacat gtactcgacg cagcgctggc agccggtctg gacctgccgt tttcgtgcaa
     4381 gggcggtgtc tgctgcacct gccgcgtgaa agtgttgcaa ggcagcgttg cgatggacaa
     4441 gaactttggc cttgaagcct gggaaaccga ccagggtttt gtcctgagct gccagtcgcg
     4501 cccgaccagc caggccgtca ctgtgagtta tgacgagcgc tgacacgaag tgagcgcggc
     4561 cgctgctgtg atcgctgcga aaaggcctga accgtggctg caccgtgagt tgcccggtgt
     4621 agtgattttg gggtcggatc ccaattgcgt cggcacacgc ccaatgcaaa aaacagtgcc
     4681 aataagcaat tgggctcaga ccccaatatc acgggccatt accctgagcg ctaacaaaag
     4741 caatagctgc cagcgcttat tccatggggg ctgagcccaa tttccagcgc ggccgtgttg
     4801 cgcgacgact gaaccatcgc caccagttaa acaaggggat acatggtact gattttgggg
     4861 tcggatccca attgcgtcgg cacacgctca atgaataaac agtgccaaaa agcaattggg
     4921 ctcaggccgc aatatcacgg gcaatcgccc aaatagctac caaaacagta gcttccagcg
     4981 cttattccat gaggtctgga ggccaatttc tcttgaaatt ccaaaaaatg aaacgaaccg
     5041 gcacagcggc atcgctgcgg cctatgcgag acgacagggc tttcctgggc cggtgaaatc
     5101 ggggtctgat tccatttcta gatcatccaa atcgttgttg tcccgccttg ccttgcgctt
     5161 gcaaggtcag cggcaacgat ccagagcgca aatgattcag taatgccatg acaagacgcg
     5221 ctcaagctgt tgatttgccc tgtcccaaaa ttttgctcaa tcaccggcac ttgcgttggc
     5281 gcgaggtcag cttggcatga gccgatgagg atcagcaagt tcaaagggca agggcagcga
     5341 tgccatcacg ctcaggatcg cccggatcac cagatccggg cggtcacggt ggacgtggtg
     5401 accactttgg tcacaaattc tgagtagccc ctgggccgac atgccggcaa ggctgctttg
     5461 acaatccgcc caggccagtg ccagcccccg tgtctggtac aaaaaacgcc gccgatgggt
     5521 caacacccgc agtggcacgt tcgggaatgt cccggttgat cgcgcctgct ccatggtggc
     5581 cggcgctgcc agcagctcgc tacgcattgc cgagcgaccc agcagaaaca gcggaagggg
     5641 aggttcgtag agcaggcgca gcaagcccat ttttcggcag cgcccaacga agtcggcatg
     5701 tcgggcatcc accagaacca cgcctgccac ttcatcggga taggtccgtg caaacaactt
     5761 gacgagggtt ccgcccaaag agtggcctac caggacatag ggtggggcga tgttctcggt
     5821 tcgcaatagt gccctgagtt cgtgaacgat ttgaaagccg tcgcgggtcc aggacgattt
     5881 gtcgctttgt ccatacccag cccggtcgta ggccagcacg cgggtcttgc gactcaaagc
     5941 gttgaaaacc ggtgcccaca cttctttgcc atcacccagg ccagattcga atatgacggt
     6001 gggcgagcct gatccggcca aataatagga acgcgtgcca aacggggtgt gactgaatcg
     6061 cacgggcgat tgagccttga gctgatgatc cgcgcagggt tttacagatg tgccgaacaa
     6121 acgatgcatc agcccggcca gccacggaag ctccatgaca aactgatcag tggcacctag
     6181 catttcaatc atcccaagtt tctcagttgg acgcgcccga gtgggccgct ggcggcgcgg
     6241 ctggctaggg cgaaagctgc ggctccaccc gtgcctgcgc aggtttggac ggcctgaagc
     6301 aggccagcct tgtggcatgg ccgtgccgtc gcagcaaagg gtgaaaggcg acgacgacgc
     6361 tcagctactg agagcatgtc gggccgacgg aggtgggtgg ggtgaagtcc ccaggcgagc
     6421 tgccagtgct ttgatcgggt gcgcaagatg acccaatggg tgaatccagt cgaagccaaa
     6481 aagtaaatgc tcataagaac ttacagagga aaacaaacgg tcaactgagg aatccaggct
     6541 catgtcagaa caatcctgcg gcgctttgcc ttgacaacat tttgcatttc atattcatcg
     6601 atcaattggg gccagtttga cacatcatgg gctgcaactc taaccaagaa cgcggccatt
     6661 ttctgttgca aagggaacga ataagtaaca aattgtgttc ggcaatgctg cgtgcccgta
     6721 gcgattggcc gttggcgcaa ggcatcttgc tgacaccctc gttcaagctg actgatacag
     6781 ccctgcggca accccagcga tcccaaaaag gatcggtgcg ccgacgagca gcgctgggca
     6841 ggtgttgttt ggccgaaaaa gtccgttcgg ggcaatgcag gtgacgttgc gccccgccag
     6901 gaaaagtttg ttgccgtgca actcaatcat tccagcgaag gccgcaaccc aaaaccgcgc
     6961 ggggagcttg tcgggcttgt ggcgcatcgg ctgctaattg aacgcagtgg cccattttgc
     7021 accggttttc cgacccgcaa ccggggcgaa accggtttgc gctgcgacgc gaaaactcag
     7081 aacttcggca aatgaaactt tggcggcacg cccattcggg tgttgatgac ccactgctgg
     7141 gcaatcgaca gcacgttgtt ggtgatccag taaagcacca ggccggacgg gaaaaagaag
     7201 aacatcacgc tgaaggccag cggcatgaac cacatcatct tggcctgcat cgggtcgggc
     7261 ggggccgggt tcagcgaggt ttgcaacacc gtggtcaagg tcatcaccag cggcaaaatg
     7321 taatacgggt cgggcgagga caggtcatga atccagccca gccagggcgt gttgcgcatt
     7381 tcgacgcttg acagcagcac ccagtagagt gcaatgaaga ccggaatctg gatcatgatc
     7441 gggaaacagc cgcccatcgg gttgactttt tcctcgcggt agatgcgcat catctcctgt
     7501 tgcatctgct gcgggttgtc tttcaggcga tcacgcattt cggtgatctt cgggttgacc
     7561 gccttcatct tggccatgct ggcataagcc ttggcgttga gccagtaaaa cgctgccttg
     7621 agcaacagca ccagggccat gatggaccag ccccagttgc cgatcaacgc gtgcagtttg
     7681 accagcagcc agtaaagcgg cttggccaga atcgtcagcc agccgtagtc cttgaccagt
     7741 tccagacccg gtgccattga ctccagcacg ctttcttcct gcggaccagc aaagaagcga
     7801 gcttctaccg acttgcgcgc acccggcgca atcgcctcca gcggagcaat catgccgaca
     7861 gcatacaaat tgttgtccac ccggcgcatg aacaaatcac gcttgatgcc atcgccgagc
     7921 agccaggcgc tggcaaagta atgctgcacc atcgccacgt aaccgttgtc agccgtcttt
     7981 tcgatgtcaa tcttgttctt ttcaatatcg gcaaactcga ctttctggta cttcttggca
     8041 tcggtgtaaa cggccgggcc ggtaaaggtg gaatagaaag acgattcgcc cggcggcttg
     8101 ttgccgtccc gcaccaattg caggtaaagc tggggtgcca ctgccgccgc acccagattg
     8161 accacctcgt gcttaaccgc taccgcgtaa ctgccgcgcg ccagggtata ggtcttcacc
     8221 aacttaacac caccgacttc tggcgactca aaacgcaact ccagactctg gctgccatcc
     8281 tttagcacgc gctcgccggg aaccagcgtc atcaccgact tgtggttcgg caaggcagca
     8341 ccaccggcca gcgcaatcag gccagactgc gccagataga cgcgatcttt gctgtcgtca
     8401 agcaagacga attggcgcgc cttgtccgcc atgtccatgt gcttgagaaa ttctgtgcgc
     8461 accaccgagc cgccttcggt gtcaaacgtg accttcagta cgtcggtctc ggcgaccacc
     8521 tgctggtgcg ccaccgaact gctgacttga ggcactaatt cggctgtagc ccccgttggc
     8581 cgtgcgccta cagctacatt agtagtagca actggaacac tatcgacgcc acttgcggtc
     8641 ttggatggtg ccggtgcctg ggcgactgtg ggtgccggtc ctgggaaaaa agtcggcttg
     8701 ttgccattgt gaatctgcca tttgtcccac agcatgacca tggaaaaacc aaaaatcacc
     8761 cacaaaatag tgcgccgaat atcgttcatg aggacttctt ttgagtggta gaggaaatta
     8821 accgggtaag caaaccagac cgggcaagcg gggccgcgcc gggcaccgga tcgaagccac
     8881 cgtcacacca tggatggcag cgcccgatgc gtccaagggt caaataactg ccattgagcg
     8941 caccatgctg ttccagggct tgcagtgcaa aagctgagca ggtcggctca aaccggcacg
     9001 atgagcccaa cgacgggctc agcagcagcc gatatgcctt gaccaatctg atcagaaaac
     9061 gcgcaatcat ggcaagcccg tcttcatgcg acccctggtt gcactgtcaa tggcggcgcg
     9121 gtcgcggctt ttgaaaacaa ctgctgcagt tcctggcgca cggcaatttt caaggccagc
     9181 gacgtggcac tgacaaaatg ctggcgatca aagctgctgc gcaaacgcac gacatgggcg
     9241 gcacaggcca aattggcctc aaagtccaga ctcacggcat aaaccagacg cttgatggaa
     9301 ttacgagtaa ctgcccgttt ggcccagcgc ttgggcacca tggcaccgag ccagacatcg
     9361 cgcttggcaa acaagggcga tgcatcaggt gccgatggcg ttgacccgga caatgccaca
     9421 cggtgcaagg caaaatgcgg cgttcggacc aaagtcacac cagccaaaac cgcctgaaat
     9481 tgcgggcggg ttttaagacg ttgcaaagcc agcttgcacc cgccgacaat cagaccgcca
     9541 gacgcttgcg gcccttggca cgacgggcgt tgatgacagc gcggccacca cgggtcttca
     9601 tgcgaaccag aaagccgtga gtacgggcgc ggcggatttt ggaaggttgg taagtgcgtt
     9661 tcattatttg gaaatcctaa gagtctctgg ggcgatgcag cgtgcgccat cgaagatgcc
     9721 gcagcttgaa tcagtgttca gatttttcct gaaccattca gggaacctcg gattatcgca
     9781 aactttgcgt agcggctctg ccctgtcggt aatcccggct gcgtcaggct ctggcggacg
     9841 gctgtgcagc gcgattcacc agtgttggcc agatgcccgg ctctgggccc gcgatgaaca
     9901 ccttggcttt caaaccgtcc agcactggca aattcagcgt tgctgccctg gcacagcagg
     9961 gaatcttgtc tggctaactt tcatggactc cattcatccc cggacatgaa agtgatttca
    10021 cgttaatccg gcttgccctt tgctgcccgc ttgctgcggg ctgtcacgcg ggcgctgtat
    10081 gaatcactga gggtgaattc cccgccactt ggggcgatgc caatgcggcg cagcgaccaa
    10141 tttcagggat tgtggcgggt ggacgaagaa acagtgggca atgcttggca gcgcggtgtt
    10201 ggccgggtcg gggcattgag cccggtctgc cacctgaccc ggtgccagca cgtcgctgcc
    10261 gccatgcgtc caccggctcg tctcacctgt tttcagactt gcatagtgct ctcaaatatt
    10321 gagctgccag cataatcaac atgcgggctg gaaggacttt tgatgcatac gagacggtcc
    10381 gagcaatggc tggcattccg gatattcagg gccattgccg ccgctggcca gccactgttg
    10441 gacgaccggg ttttggcggc ttaaggtgcc acctcggccc agcgcaggcc gcaggtcaca
    10501 cccagcaacc aggcggtcag acgcgccgcc cacttgcccg cccgttcccg gttttgccca
    10561 tggggcagcc ccttgtagcc gcactggaca tcggtgatgt tggcaaaacg gaaagcaccg
    10621 cacctggcag acccagccgc acaagccaga gcattggtgc ctccacctgg tgtttgaggt
    10681 tttggccaat gacgcgatgg cactttcacg cacctgcacc tggctcaagg cggcagtgtc
    10741 gcggctggcc agccagttca ggcgttcgcg tccggcttca cgcgatggct ctagagcggt
    10801 ctccactgct tgcacctcgt ctttgagcat gacccaggcc agcaggggtt tgagcaacaa
    10861 cgccagccat acgttagcca acggcagtgg caaggctgtc aaattatctg caccagcgat
    10921 tgcagcgggt ccacctggca gactctgcat agctgaaaag cagcatttcc cagccaccag
    10981 agtgctattg aaagcaaagc agctagcgca tactggtaat ggactgcaag ccaaaagcca
    11041 ttgaactcag gaaagttatg agcatcccgc atcagcggcc cctcaagtca gccgggcagt
    11101 cggcccgggg cggccttcca agccatcctc tgaacccggt taacagcgca ggtcgttgct
    11161 cggccacacc accggatgcc agcacagcgc cggtttgcca aaccggcggt caataaacag
    11221 cgccagcagc aaggccagca tgccgatgaa agcggtcatg aaaaccacgg cacgacaaaa
    11281 ccagccccgc cggcgtgcca attgtcagga ctccgcagcg tcagcccggg catgggcatg
    11341 ggcatgggca catgcttgcc gaaaaatcag ccctcaatat cgccttgtgc aaccgaagtg
    11401 gcagactttg acgcaacgtt tttgcaccag gccatgatga tgcggtgcag ttgcgccacg
    11461 ccgtcggtta gggcggcggg gccaggttgc aggatgtcgg gcgacttgat ctcgaacagt
    11521 tgcccggtct tcaccgccgc aacctcgctc cagccagggc gtgctgccac gttttccggg
    11581 cgaaacttct tgccgcacca ggagccaatg atgatgtcgg gg
//
LOCUS       DAOSLL010000063        11592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5544_length_11592_cov_5.153246, whole genome shotgun sequence.
ACCESSION   DAOSLL010000063 DAOSLL010000000
VERSION     DAOSLL010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11592
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5544_length_11592_cov_5.153246"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2107
                     /locus_tag="PLB25_04270"
     CDS             <1..2107
                     /locus_tag="PLB25_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HOE40833.1"
                     /translation="NYEIDIFDALIKAAARETACPDLTNNSLKVIADHIRATAFLVSD
                     GVLPGNEGRGYVQRRIIRRAIRHGYKLGQKTPFFHKLVPDLVAQMGDAYPGLAAQSAR
                     IMEVLRVEEERFYETLANGMEILETEFDMLEIKSNMTNEELATYELNSMKIHMGSNIF
                     GSYTAKPISGQGELLFFYVVDEGEEDAANQDAESESAFPDHRGNRIIVRGRLQKNPLS
                     GVVAFKLHDTYGFPLDLTNDVCRERGVVVDSAGFDAAMARQKSQGRAAGKFKQDRALE
                     YHGAGNAFLGYEQFTVATEIVAIYADGVSATALNAGQSGVLVLAATPFYGESGGQVGD
                     AGAIFSDLAMFDVADTQKIKSDVFGHYGTLKSGALKVGDQVTAHVNASLRLATMRNHS
                     ATHLMHKALREVLGSHVQQKGSLVNPERTRFDFAHNTPVSDGEIREIEARVNAEILTN
                     AATQARVMAIEEAKKTGAMMLFGEKYGESVRVQDIGSSRELCGGTHVQRTGDIGLFKV
                     VAESGVASGVRRIEAVTAVNALLYMQQLEDSVNQAAGVLKAPVAEVVDRIVTVLEHVK
                     TLEKEVTALKGRLASAQGDELVAQAQDINGVKLLVARLDGADVKTLRDTMDKLKDKLK
                     TAVIVLAVVDADKVQIAVGVTANTTGRVKAGELVNFVAAQVGGKGGGKPDLAMAGGTE
                     PGKLAAALASVAGWVTPRL"
     gene            2150..3097
                     /gene="hemC"
                     /locus_tag="PLB25_04275"
     CDS             2150..3097
                     /gene="hemC"
                     /locus_tag="PLB25_04275"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HOE40834.1"
                     /translation="MSKFTIATRQSRLAMWQAEHVQSRLQQLGHQVALLGMTTLGDQI
                     LDRTLSQIGGKGLFVKELEVALEQGHADLAVHSLKDVPMELPDGFSLACVMEREDPRD
                     AFVSNRFASLDALPLGALVGTSSLRRVALLRALRPDLKIEPLRGNLDTRLRKLDEGLY
                     DAIVLAAAGLKRLGLQARIAAVFEPATMLPAAGQGVLGLEVRSDRPDVALALAALAHH
                     DTWLRVAAERAVSRAMGGSCSMPLAAYATLEQDQLVLHAAWGDVHGHLPLARPAPAAA
                     VTDQAGAAALGQRVADALRASVQAQGGGVALSASAAPAT"
     gene            3111..3932
                     /locus_tag="PLB25_04280"
     CDS             3111..3932
                     /locus_tag="PLB25_04280"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HOE40835.1"
                     /translation="MRVIITRVQPQAQHWVDGLQRAGHHAIALPLIETRALSDCTALV
                     NAWQRLADYAAVMFVSGPAVAYFYASKQPLGLMDNALEAPIKRAWATGPGTRNALLAQ
                     GVPAELIDSPAAEAGQYDSEALWQQVQQQLLPGQRVLIVRGDSRSDDGPTAAPDNAAG
                     IAGVGRDWLARHLQSAGILVDSVVAYRRGAPVWSAEVALLASAAATDGSIWVFSSAEA
                     VANLQTLLRKQSWAKARAVATHQRIAAAVRALGFAVVLLASPTLPSVLASVESCP"
     gene            3929..5035
                     /locus_tag="PLB25_04285"
     CDS             3929..5035
                     /locus_tag="PLB25_04285"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOE40836.1"
                     /translation="MTVATPSDLPNPEPPDRLPADPGDASAVANPALSAPTNAAPRRN
                     LSLRLALGAGALAGLGLLLGVLLWQKVNNMQEHLARQSADAQSQSLEARTLARQAVAL
                     AQETAAHAAVLDSRLSEVALQRSQLEELMQSLSRSRDENLVVDIEAALRLAQQQAQLT
                     GSVEPLLAALKSAEQRVVRAAQPRLALLQRAIARDIDRVKASAVSDTPTMLIKLDELV
                     RMADDLSLANAVTPANLVQEAQKARNASAQTWWQTGLALVLDEARNLVRVSRVEVPEA
                     ALLSPEQSFFVRENFKLKLLNARLGLLSRQIESARADLAVAEDSLKKYFDRSARLTQA
                     AALLLQQVQGQMRNLQLPRVDDTLAVLATAAAGR"
     gene            5038..6333
                     /locus_tag="PLB25_04290"
     CDS             5038..6333
                     /locus_tag="PLB25_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HOE40837.1"
                     /translation="MRAAIWFLGLFGVAVALALFASGNDGTITVYWPPHRVDLSLNLV
                     LLLLGLLFALMHVALKALAALVAIPGQAHSWRVRHQERVIHSALLDALSHLIAGRFIR
                     ALKAAELVLAREAAMAHSGAASTDTTRLQVLAHLLAAESAQALQDKTARELHFQQALA
                     LATSHDASGTREGVLLRAVRWSLEDRDAAAALDWLDQLPSGVARRTVSLRMRLKVTRL
                     AGKTDSALETARLLAKHRAFSENAALSLVRALALEMLQTAHDPARLEALWGKLEAAEK
                     NLPQVAMAAAGRLLRLGGDAHLAFQWLLPVWERMVNPEQGLAQEDRIFLIRVMEQAFT
                     TTEDAIDTVWLARIELAQRTQPGDAALLYLAGVACLHLKLWGKSQQLIRQSLPRLNDA
                     SLLTRAWQVLAELAQREGDDAQAALAWKNAAHASLSRRP"
     gene            6408..7484
                     /locus_tag="PLB25_04295"
     CDS             6408..7484
                     /locus_tag="PLB25_04295"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE40838.1"
                     /translation="MTTFIEHIVKLTDHRDRDLLELTLAKALIDFLPVGRIVIARVMR
                     EEGVERWLEVARLDAKGGGRVIDPTRVDFQSLVKLEDAGDRLKCLQSRERVEIDWAGP
                     DGPRINMLPLFSDSRSDDQGVLELHSDAALRAEDLLLVDCLRRIYRNMYTLLEYSDRD
                     PLTGLLNRKSLDDAFYSAVLEDLDRTTAAPAVTDLPGQERRHRVPVNYWLGSASVDNF
                     ALIHDKHGRAVADDVLQLVARIMSNTFRTYDRLYHFRAEDFGALMHCPDEALVLSAFD
                     RFRANVEKFRFAAVGRVTVSCGFTSVMADDSPKTAIEKTQLAVDFARRGGGNKACSHL
                     GLVQRGFFGGPSSRRSVARDEEIM"
     gene            complement(7530..10493)
                     /locus_tag="PLB25_04300"
     CDS             complement(7530..10493)
                     /locus_tag="PLB25_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HOE40839.1"
                     /translation="MKRMLINATQAEERRLAVVDGQKLLDYEIEIEGREQRKGNIYKA
                     VVTRVEPSLEACFVDYGEDRHGFLPFKEISKQYFQAGVAVSQARISDVIKEGQELLVQ
                     VEKEERGNKGAALTTFVSLAGRYVVLMPNNPRGGGVSRRIEGEDRAELKEALDQLEYP
                     NGMSIIARTAGIGRSAPELQWDLNYLLKLWSAIDGAAKGGKGAFLIYQESSLVIRAIR
                     DYFNHDIGDILIDTDDVYDQAQQFMAHVMPEHAARVKRYRDDAPLFSRFQIEHQIESA
                     YARTVTLPSGGAIVIDHTEALVSVDVNSARAIKGGDIEDTATRTNLEAAEEVARQARL
                     RDLGGLIVIDFIDMEESRNRRDVENRLRDALRQDRARVQFGTISKFGLMEMSRQRLRP
                     ALSEGASIPCPRCGGSGHVRDTESSALQILRIIQEESLKDNTASVLCQVPVEVASFLL
                     NEKRTEIAKIELKQRINVLMVPNKSLETPNYRLERLKHDDPRLDNITPSYKMADEVED
                     AIAVTRRSQEPTNRQTPLIKGVLPDAPAPAAPVRPEPAAVAPPAKPEAVTPVVVEKGF
                     FGWLKNLISPVPVPAPVAPPPAAPLVEEPRNNRDGRPARDGSRRNEPRGDRPDGRGPR
                     TDGPRTDGRGGRTEGRGDGRNEGRPEGRGGRGERGNRNERNSGPRERTDTQAAATSAE
                     SVGVNLDAQAQGTNPEASRSDARPERNSRNGGRGDRRPPRGPKPADEAGLDDTGNPAA
                     DADVTPTAPAEPVSIEADTGNRADTAPREKRSRDRYGRDRKGRGERSERVEAGSEASS
                     LPQSQDGAAAVQEEDAPRKSYFAMPANGQPVAGVDALPLDRPAPIAAGVVPQAMAVPL
                     ADKLQASPPPAPTTQAMIVLPAAPAPAAIAVVASKLVDPPMAATMPPVAAYHLSLEDL
                     SQLAQQSGLTWVNSNAEKVAAVQAAIAAEVQPARIPRERPPAVAGDDRPLILVETKRD
                     LRNVQLPFENAELTS"
     gene            11062..>11592
                     /locus_tag="PLB25_04305"
     CDS             11062..>11592
                     /locus_tag="PLB25_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOE40840.1"
                     /translation="MKTVCVDEDGIGQRLDNFLMRELKGVPKSHVYRMIRSGEVRVNK
                     GRAGADTRLVAGDMVRVPPVRVSQRMTEKQQDMAARVASGVPCRDFAVLLEDEHLIAL
                     NKPAGVAVHGGSGVSFGVIEQLRMARPAARFLELVHRLDRETSGILLVAKKRSALTNL
                     QDQFRQRQTGKTYLALV"
BASE COUNT         2184 a         3348 c         3715 g         2345 t
ORIGIN      
        1 caattatgaa atcgacattt ttgacgcgct gatcaaggcc gcggcacgcg aaaccgcttg
       61 ccctgacctg accaacaact cgctcaaggt gattgccgac cacattcgcg ccaccgcctt
      121 tttggtgagc gacggcgtgc tgccgggcaa cgaggggcgc ggctatgtgc agcgccgcat
      181 catccgccgc gccattcgcc acggttacaa gctgggccag aagaccccgt tttttcacaa
      241 gctggtgccc gatctggtgg cacagatggg cgacgcctac cccggcctgg cggcacagtc
      301 ggcgcgcatc atggaggtgc tgcgggttga agaagagcgt ttttatgaga cgctggccaa
      361 cggcatggag attcttgaaa cagagttcga tatgcttgaa attaaatcga acatgaccaa
      421 tgaggaactc gccacatatg agttgaactc aatgaagatt catatgggct cgaacatatt
      481 tggctcatat actgccaaac caatttctgg ccagggcgag ttgctgtttt tctatgtcgt
      541 tgatgaaggc gaagaagacg cagctaacca ggatgctgag agtgaaagtg cttttcctga
      601 tcatcgtggg aatcgaatca tcgtgagagg gcggttgcaa aagaacccac tttccggtgt
      661 agtcgctttc aagctgcacg acacctacgg cttcccgctg gatctgacca acgatgtctg
      721 ccgcgagcgc ggggttgtgg tggattcggc cggttttgat gcggccatgg cgcggcaaaa
      781 gtcgcagggg cgcgcggcag gcaagttcaa acaggaccgg gcactggaat accacggtgc
      841 gggcaatgcg tttctgggct acgagcagtt cacagttgct actgaaattg tagctatcta
      901 cgcagacggg gtaagcgcta cagccctgaa tgctggtcaa tccggggtgc tggtgctggc
      961 tgccacgccg ttttatggcg aaagtggtgg ccaggttggt gacgctggcg cgatcttttc
     1021 tgatttggcc atgtttgatg tggccgacac gcaaaagatc aagtccgacg tgtttggcca
     1081 ctatggcacg ctcaaatcgg gcgcactcaa ggtgggcgat caggtcacgg cacatgtcaa
     1141 cgccagcctg cgcctggcca ccatgcgcaa ccactcggcc acgcacttaa tgcacaaggc
     1201 actgcgtgag gtgctgggca gccatgtgca gcaaaaaggc agcctggtga acccggagcg
     1261 cacacgtttt gactttgccc acaacacccc ggtaagcgac ggagaaatcc gcgaaattga
     1321 agccagggtg aatgccgaaa ttctcaccaa cgcggcgacc caggcgcgcg tgatggccat
     1381 tgaagaagcc aaaaaaaccg gtgccatgat gttgtttggc gaaaaatatg gcgagtcggt
     1441 gcgggtgcag gacattggca gcagtcgcga actgtgcggt ggcacccacg tgcagcgcac
     1501 cggagacatt ggcctgttca aggtggtggc agaaagcggt gtggcctctg gcgtgcggcg
     1561 cattgaagcc gtgaccgcgg tcaatgcact gctctacatg cagcaactgg aagacagcgt
     1621 gaaccaggcc gccggcgtgc tgaaagcgcc ggtggccgag gtcgttgatc gcatcgtgac
     1681 ggtgctggag cacgtcaaga cactggaaaa agaagtgact gcgctcaagg gcaggctggc
     1741 atccgcccag ggcgacgaac tggtcgcgca agcgcaggac atcaacggtg tcaagctgct
     1801 ggtggcccgg cttgacggtg ccgatgtcaa aaccctgcgc gacaccatgg acaagctcaa
     1861 ggacaagctg aaaactgccg tcatcgtgct ggcagtggtt gatgctgaca aggtgcagat
     1921 tgctgttggc gtgacggcca acaccaccgg gcgggtcaag gccggagaac tggtcaactt
     1981 tgttgccgcg caagtcggtg gcaagggcgg cggcaagccc gacctggcca tggctggcgg
     2041 caccgagcca ggcaagctgg ctgcggcgct ggccagtgtt gcaggctggg tcacgccgag
     2101 gctttgacac gatgcaattg atcaaaataa ccacaacggt gtctggctca tgagcaaatt
     2161 caccatcgct acccggcaaa gccgtctggc catgtggcag gccgaacatg tccagtccag
     2221 gctgcagcaa ctgggccacc aggtggcgct gctgggcatg accacgctgg gcgaccagat
     2281 tctggaccgc acgctcagcc agatcggtgg caagggcctg tttgtcaaag agctggaagt
     2341 cgcgctggag caggggcatg ccgatctggc cgtgcattcg ctcaaggatg tgccgatgga
     2401 actgcccgac ggcttcagcc tggcctgtgt gatggagcgt gaagacccgc gtgatgcctt
     2461 tgtgtcaaac cgctttgcca gccttgacgc gctgccgctg ggggcactgg ttggcacctc
     2521 cagcctgcgt cgtgttgcgc tactgcgtgc gctgcggcct gatctgaaga ttgagccatt
     2581 gcgcggcaac cttgacacgc gcctgcgcaa gctcgatgag ggcctttatg acgccatcgt
     2641 gctggctgct gccggactca agcgccttgg cctgcaggcg cgtattgctg ccgtgtttga
     2701 gcccgccacg atgctgcctg ccgctggcca gggtgtcctg gggcttgagg tgcgcagcga
     2761 ccgcccggat gtggcgctgg cgctggcagc gctggcgcat cacgacacct ggctcagggt
     2821 cgctgcagag cgcgccgtca gccgtgccat gggtggcagt tgttcgatgc cgctggccgc
     2881 ctatgccacg ctggagcagg atcaactggt gctgcatgca gcctggggcg atgtgcatgg
     2941 tcacctgccg cttgccagac ccgcaccagc ggcagcggtg accgatcagg ctggcgcagc
     3001 agccctcggc cagcgggtcg ccgatgcgct gcgcgccagc gtgcaagcgc agggtggtgg
     3061 tgtggcgctg tctgcgtcgg cggcaccagc aacttgagct gattgaatcc atgcgcgtga
     3121 tcatcacccg ggtgcagcca caggcacagc attgggttga cggcttgcag cgcgctggtc
     3181 atcacgcaat tgccctgcct ttgattgaaa cccgcgcttt gtcagactgc actgcgttgg
     3241 tcaatgcctg gcagcggctg gctgactacg ctgccgtgat gtttgtaagt ggccctgcag
     3301 tggcctattt ttatgcatca aaacagcctc taggccttat ggataatgcg ctggaagctc
     3361 ctattaagag agcatgggcg accggcccgg gaacccgcaa tgccttgctg gcacagggtg
     3421 tcccggctga actgatcgac tcgcctgcgg ctgaggccgg gcaatacgac tccgaggcgt
     3481 tgtggcaaca ggtgcagcag caactgctgc caggccagcg cgtgctgatt gtgcgcggcg
     3541 acagccgatc agacgatggc ccgacggcag ccccggacaa cgccgctggc atcgcggggg
     3601 tcgggcggga ctggctggcg cggcatttgc aatcggccgg catcctggtg gactctgtcg
     3661 tggcctaccg gcgtggcgcg ccggtctgga gcgctgaggt tgcgttgctg gccagcgccg
     3721 cagccaccga tggcagcatc tgggtgttca gcagcgctga ggccgtggcc aatctgcaga
     3781 ccctgttgcg aaagcagagc tgggcaaaag cccgggcagt cgcaacccat cagcgcattg
     3841 cggcggcggt gcgggcactc ggctttgcgg tggtgctgct ggcaagtccg accctgccca
     3901 gtgtgctggc ctcggtagaa tcatgcccat gaccgtcgcg acaccttctg atctacccaa
     3961 ccctgagccg ccagaccgtt tgcctgccga tccgggcgac gcaagcgctg tggcaaaccc
     4021 tgcactttca gcaccaacaa atgctgcacc acggcgcaac ctgtcgctgc ggttggcgct
     4081 gggggctggc gcgctggccg ggctgggttt gctgttgggg gtgttgctgt ggcaaaaagt
     4141 caacaacatg caggagcact tggcgcgcca aagtgccgat gcgcagtcgc agtctcttga
     4201 agcccgtacc ctggcccggc aggcagtggc gctggcgcag gaaaccgctg cgcacgctgc
     4261 cgtgctggac tcgcgcctga gcgaagtggc cctgcagcgc agccagttgg aagagctgat
     4321 gcagagcctg tcgcgctcgc gcgatgaaaa tctggtggtt gatattgaag ccgcactgcg
     4381 cctggcacag cagcaggccc agttgaccgg cagtgttgag ccgctgctgg cagcactcaa
     4441 aagcgccgag cagcgcgtgg tacgggcagc gcaaccgcgg ctggccttgc tgcagcgcgc
     4501 cattgcgcgc gacatcgacc gcgtcaaggc ctctgcagtg agtgacaccc cgaccatgtt
     4561 gatcaagctc gatgaactgg ttcgcatggc cgatgacctg tctctggcca atgcggtgac
     4621 accggcaaac ctggttcagg aagcccagaa ggcccgcaac gcaagcgcac aaacctggtg
     4681 gcagactggt ttggcgctgg tgctcgatga ggcccgcaac ctggttcggg tcagccgggt
     4741 ggaagtgccc gaggcagccc tgctgtcgcc ggagcagtcg ttttttgtcc gggaaaactt
     4801 caagctcaag ctgctcaatg cgcggctggg cttgttgtcg cgccagatcg agtcggcgcg
     4861 cgccgatctg gcggtggcag aagacagcct gaaaaaatac tttgaccgca gtgcccgcct
     4921 gacccaggca gcggcgttgc tgttgcagca ggtgcaaggg cagatgcgca atttgcaact
     4981 gccacgggtt gacgacacgc tggcggtgct ggcaaccgct gctgcagggc gctgacaatg
     5041 cgcgctgcca tctggtttct tggcctgttc ggggtggcgg tggcgctggc gctgtttgcc
     5101 agtggcaatg acggcaccat cacggtctat tggccaccgc accgggtcga tttgtcgctg
     5161 aatctggtgc tactgttgct ggggctgctg tttgcactga tgcatgtggc acttaaagcg
     5221 ctggcagcgc tggttgccat acccggccaa gcccattcct ggcgcgtgcg ccatcaggag
     5281 cgggtgattc actcggcact gctcgatgcc ctgtcgcacc tgattgccgg gcgctttatc
     5341 cgggcgctca aggcggccga actggtgttg gcacgtgaag cggcgatggc ccacagtggt
     5401 gctgccagca ccgacacaac gcgacttcag gtgctggcac atttgctggc agccgagagt
     5461 gcgcaggcct tgcaggacaa gacggcgcgt gagctgcatt tccagcaggc gctggcgctg
     5521 gcgacttccc acgatgccag cggcacgcgt gagggcgtgt tactgcgcgc ggtgcgctgg
     5581 tcactggaag accgcgatgc ggcggcagca ctggactggc tcgaccaact gccctccggg
     5641 gtggcccggc gcaccgtatc gctgcgcatg cgcctgaaag tgacacggct ggctggtaaa
     5701 actgacagcg ccctggagac cgcccggttg ctggccaagc accgggcatt ttccgagaat
     5761 gccgcgctca gtctggtacg ggcgctggcg ctggagatgt tgcagacagc gcatgatccg
     5821 gcccgtttgg aggcgctctg gggcaaactg gaagcggctg aaaaaaatct gccacaggtg
     5881 gcgatggcag ccgccggacg cctgctgcgt ctgggtgggg acgcccattt ggcctttcaa
     5941 tggctgttgc cggtgtggga gcgcatggtc aatcctgagc aaggattggc tcaggaagac
     6001 agaattttct tgatccgggt gatggagcaa gcctttacga cgacagaaga tgccattgac
     6061 acggtctggc tggcgcgtat tgaacttgcc cagcgaaccc agccaggcga tgccgcgctg
     6121 ctgtatctgg caggtgtggc atgcctgcat ctgaagctgt ggggcaagtc gcagcagttg
     6181 atccgccagt cgctgccgcg tttgaatgat gccagtttgc tgactcgcgc ctggcaggtg
     6241 ctggccgaac tggcgcagcg cgagggtgat gatgcgcagg ctgcgctggc ctggaagaac
     6301 gcggcgcatg cgagtttgag tcgccgacct tgatcctgat caaagaaccg gctgtttgag
     6361 cgcgttctcc aggtgacgaa accgggtttc aagtctatga tttgacaatg actacattca
     6421 tcgaacacat tgtcaaactc accgaccatc gcgatcgtga tttgctggaa ctcaccctgg
     6481 ccaaggcact gattgatttc ctgccagtcg ggcgcatcgt gattgcccgc gtcatgcgcg
     6541 aagagggggt tgaacgctgg ctggaagtgg cccggctaga tgccaagggc ggcgggcggg
     6601 tgattgaccc gacacgggtt gattttcagt cgctggtcaa gctcgaagat gccggtgatc
     6661 gcctgaaatg tcttcagtct cgtgaacgtg ttgaaattga ctgggccggg ccggatgggc
     6721 cgcgcatcaa catgctgccg cttttttcgg attcccgctc ggacgatcaa ggtgtgctcg
     6781 aactgcactc agacgcagcc ttgcgcgccg aagatctgct gctggtggac tgcctgcgcc
     6841 gcatttaccg caacatgtac accttgctgg aatacagcga ccgcgacccg ctgactggcc
     6901 tgctcaatcg caagtcgctt gacgatgctt tttacagcgc agtgctggag gaccttgacc
     6961 gcaccacggc ggcaccggcg gtaactgatc tgccaggtca ggagcgacgg caccgggtgc
     7021 cggtcaacta ctggctgggt tcagccagtg ttgacaattt tgccctgatc catgacaagc
     7081 atggtcgtgc cgtggctgat gatgtgctgc aactggtagc ccgcatcatg agcaatacgt
     7141 ttcgcaccta tgaccgtctt taccattttc gtgccgagga cttcggcgcg ctgatgcatt
     7201 gtccggacga agcgttggta ctgtcagctt ttgaccgttt tcgtgccaat gtcgaaaagt
     7261 tcaggtttgc cgcagttggg cgagtgaccg tgagttgcgg ctttaccagt gtcatggccg
     7321 atgactcgcc caagaccgcc atcgagaaaa cccagctcgc ggtggatttt gcacgccgag
     7381 gcggtggcaa caaggcctgc agccatctgg gtctggtgca gcgtggtttt ttcggtggcc
     7441 caagcagccg acgtagtgtt gcgcgtgacg aagaaataat gtgaaaaagg gcaccactgg
     7501 tgcccttttc aactgttggc tagcgctggc tagctcgtca gttcggcgtt ttcaaacggc
     7561 aactgaacat tgcgcaaatc gcgtttggtt tccaccagga tcagtgggcg atcatctccc
     7621 gcaacggctg gtggccgctc gcgcgggatt cgtgccggtt gcacttctgc cgcgatggcg
     7681 gcttgcacgg cagctacctt ttcagcgttc gaattgaccc atgtgagacc cgactgctgc
     7741 gccagttgcg acaagtcttc cagcgacaaa tggtaagccg ctaccggggg catggtggct
     7801 gccatcggcg ggtccaccag cttgctggca acaactgcaa tggctgctgg cgcaggtgcc
     7861 gctggcaaca cgatcatggc ctgggttgtc ggcgcgggcg gtgggctggc ttgcaacttg
     7921 tcggctaaag gaactgccat cgcttgcggc acaacgccag cagcgatcgg tgccgggcga
     7981 tccagcggca atgcgtcaac tccagccact ggctggccgt tggcaggcat ggcaaagtaa
     8041 gacttgcgtg gcgcatcttc ttcctgaacg gcagcagcac catcctgact ctgtggcaat
     8101 gaagaggcct cggaacccgc ctcaactcgt tcactgcgtt cgccgcgtcc tttgcgatca
     8161 cggccatagc ggtcgcgtga ccgtttctct ctgggcgcgg tatcagcacg gttgccggtg
     8221 tcagcctcga tgctcaccgg ttcggctggc gcagtcgggg tcacatcagc atcggccgca
     8281 gggttgccgg tgtcgtccag cccggcctca tcggcaggtt tgggtccacg cggcgggcgt
     8341 cgatcaccgc gcccgccgtt gcggctattg cgctcaggcc gggcatcact gcgtgaggcc
     8401 tctgggtttg tgccttgagc ttgcgcatcc aggttaacac cgacagattc ggcgctggtt
     8461 gctgcggcct gggtgtctgt tcgctcgcgc gggccgctat tgcgctcgtt gcggttgccg
     8521 cgttcgccgc gaccgccgcg tccttcgggt cggccttcgt tgcgcccgtc gccgcggcct
     8581 tcggtccgcc cgccgcgccc gtcagtgcga gggccgtcag tgcgcggacc gcgcccgtcc
     8641 gggcgatcgc cgcggggctc gttgcgtcgg ctaccgtcac gagctgggcg gccatcccgg
     8701 ttgttgcgcg gctcttcaac cagcggcgca gcaggtggcg gcgcaaccgg tgcaggcaca
     8761 ggtaccggtg aaatcaggtt cttgagccag ccaaaaaacc ccttctccac aaccaccggg
     8821 gtgactgctt caggcttggc gggcggtgcg actgcagcgg gttccggccg gaccggtgcc
     8881 gccggtgccg gcgcatcagg caacacccct ttgatcaacg gcgtttgccg gttggttggc
     8941 tcctgtgatc gacgtgtcac ggcgatggca tcttcgactt cgtcggccat tttgtagctc
     9001 ggtgtgatgt tgtcgagacg cgggtcgtca tgcttgagtc gttcaagccg atagttgggt
     9061 gtttcgagcg atttgttggg caccatcagc acattgatgc gctgcttgag ttcgatcttg
     9121 gcgatctcgg ttcgcttttc gttcagcagg aaggatgcca cctccaccgg cacctgacac
     9181 agcaccgatg cggtattgtc cttgagcgat tcttcctgga tgatgcgcaa aatctgcagc
     9241 gcactcgatt cggtgtcacg cacatgacca gaaccgccgc agcgagggca cggaatggag
     9301 gcaccttcgg acagcgccgg gcgcaaacgc tgacggctca tctccatcag gccgaacttg
     9361 ctgatggtgc caaactgcac ccgcgcgcgg tcttgacgca gcgcgtcgcg caagcggttt
     9421 tccacatcgc gacggttgcg cgactcttcc atgtcgatga agtcgatcac gatcagcccg
     9481 cccaaatctc gcagtcgggc ctggcgcgcc acttcttcag cagcttccag gttagtgcgg
     9541 gtggcggtat cttcgatgtc accacccttg atcgcgcgcg ccgagttcac atccactgaa
     9601 accagggctt cggtgtggtc gatcacaatc gcgccaccac tgggcagggt cacggtacgg
     9661 gcgtaggccg actcaatctg gtgttcgatc tggaagcgtg agaacagcgg cgcatcgtcg
     9721 cggtagcgtt tgaccctggc agcgtgctcg ggcatcacat gggccatgaa ctgttgtgcc
     9781 tggtcgtaca catcgtcggt gtcgatcagg atgtcgccaa tgtcatggtt gaaatagtcg
     9841 cgaatggcac gaatcacaag gctcgattcc tggtaaatca ggaaagcgcc cttgcctcct
     9901 ttggccgcac cgtcaatcgc ggaccagagt ttgagcaggt aattcaggtc ccactgaagc
     9961 tcgggggcgc tgcggccaat gccagcggtg cgcgcaatga tgctcatgcc gttgggatac
    10021 tcgagctggt caagggcttc cttcagctcg gcgcggtctt cgccctcgat gcggcggctg
    10081 acaccaccac cgcgcgggtt gttgggcatc agcaccacat agcgcccggc cagtgacaca
    10141 aaggtggtaa gtgcagcccc cttgttgccg cgctcttcct tctcgacctg caccagcagt
    10201 tcctggcctt ccttgatgac atccgaaatc cgggcctggc tgacagcaac accagcctga
    10261 aaatactgct tggatatttc cttgaacggc aaaaagccgt gtcggtcttc accgtagtca
    10321 acaaagcagg cttccagcga gggctcaacg cgtgtcacca ccgccttata gatgtttccc
    10381 ttacgctgtt cgcgcccctc aatttcaatt tcgtaatcga gcagtttttg cccgtccacc
    10441 actgcaaggc ggcgttcttc cgcttgggtg gcgttgatca gcatccgttt catgggtagc
    10501 attccttcaa ttcagttttc caacaaacag ctcatgacga gcttttaata cacaaactga
    10561 cgcattgaaa tgagaaggaa acagccggag gaagcaggct cacagagtga gtctgggcat
    10621 gggcgcgtgg aggggagcga tcgggttcgg gtttgcagtt gctactataa aaatagctga
    10681 atgcgcccat ttgacgggcg ctacaggctg acatgtttcc acatacagct caggtgcagg
    10741 ctgtaggctg gatcgcatca ggcacatcaa cacaaacata aaggcttcat tccattcaca
    10801 gcgcaaagct gtgaactttg ggtattccga atcagggttt caaggcgtcg tcttgaccct
    10861 tcaacttctc cgccattgga gacagggggt caacaatttg cgggcttggt gtcaggcggt
    10921 accgactttt ctggcgaggc aggcggtggg tgaactaaac ttcacctaaa cactgaacac
    10981 ttttcagtca aatcaaccac ttgcagcaca acgacagtga aaagcattat agggggcaaa
    11041 cccgcaacag gcagcgccca agtgaaaacc gtttgtgttg atgaggacgg tatcggacag
    11101 cgactcgaca actttttgat gcgtgaactc aaaggcgttc cgaagtccca tgtttatcgc
    11161 atgattcgca gtggcgaggt gcgggtgaac aagggccggg ccggggcaga tacccggctg
    11221 gtggcaggcg acatggtgcg tgtgccaccg gtgcgcgtgt cgcagcggat gactgaaaag
    11281 cagcaggaca tggcggcgcg ggtcgccagc ggtgtgccct gcagagattt tgctgtacta
    11341 ctggaagacg agcacctgat tgccctcaac aagcccgctg gtgttgcggt acatggcggc
    11401 tctggggtga gttttggggt gatcgagcaa ttgcgcatgg cgcgcccagc agccaggttt
    11461 ctggagctgg tgcaccggct cgaccgcgaa accagcggca ttttgctggt ggcaaaaaaa
    11521 cgcagtgcgt tgacaaacct gcaagaccag tttcgccagc gtcagaccgg caagacctat
    11581 ctggccctgg tg
//
LOCUS       DAOSLL010000064        11590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5547_length_11590_cov_4.938275, whole genome shotgun sequence.
ACCESSION   DAOSLL010000064 DAOSLL010000000
VERSION     DAOSLL010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11590
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5547_length_11590_cov_4.938275"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(47..1393)
                     /gene="deoA"
                     /locus_tag="PLB25_04310"
     CDS             complement(47..1393)
                     /gene="deoA"
                     /locus_tag="PLB25_04310"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF004490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HOE40841.1"
                     /translation="MLAQEIIRIKRDGHTLERADIEHFVRGLVDGSWSEGQAAALAMA
                     MFLRGLSRNETVNLTQAMTHSGLVMDWSTANFNAPVLDKHSTGGVGDKISLMLTPIVA
                     ACGGVVPMISGRGLGHTGGTLDKLGCIPGYQTAPDVAMLHQTLKTAGCAIIGQTAELA
                     PADRRLYAIRDVTATVESVSLITASILSKKLSAGLQGLVMDVKAGNGAFADSLEMAGE
                     LAESLVSVADGAGLPTCAWLTDMNQVLGQSCGNALEVLEAIAFLKGQSIEPRQWLLTR
                     TLSAELLLMGGLASDETDALQKVDHALASGRALERFSCMISALGGPADLADRPGHYLP
                     PAPVQRVVQAPHGGWVSSMATRDIGLLIVELGGGRRKVSDTIDTRVGFSQFAQIGQKV
                     QAGEPLAVVHAGDSAAAEHAQTALLTKIQISDKPVKPAPVMLQRVRCHRTPANGIQ"
     gene            complement(1460..2278)
                     /gene="deoC"
                     /locus_tag="PLB25_04315"
     CDS             complement(1460..2278)
                     /gene="deoC"
                     /locus_tag="PLB25_04315"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HOE40842.1"
                     /translation="MNPTTHSTSRPQPTWSHSQESSARIALACLDLTSLNDADTEADI
                     ARICKRAQGPFGPVAAVCVWPRLASFARTQLPAHIGVAAVANFPHGNADIGAAVQDTL
                     QVVQAGAQEVDVVLPFRALMAGDAATVTACISAVRQACPNLLLKVILETGELRTPALI
                     QRASELALVAGADFLKTSTGKTPLSATPEAARIMLNAIATSPVARSHVGFKVSGGIRT
                     VQDAIVYESLAAQILGVEALNPRRFRIGASSILSDIEAVLTGNIPASAAAPGSY"
     gene            complement(2575..4437)
                     /locus_tag="PLB25_04320"
     CDS             complement(2575..4437)
                     /locus_tag="PLB25_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40843.1"
                     /translation="MSLAPVVHALTMGELRGPVVIGRTLDVSVQVQAASGDDLAASCF
                     AADVFNADARQGKPKVTVTSATAESAVVRIESAGVVDEPVVSVDLSFNCGFFATRRYV
                     LLTDIESGDLAPKIATVGSVAHRAGSEQSELASGRPKRSPSGDAAPVKQKTVAQRRVA
                     VPERASNAQAGAPKPAASVSAEAVKPVVMRKSPEPATGKAVLKLGPLDDFAARISALE
                     SAKLAGPSAEVLLQTQQIASLQADIKALRDIAAKNDAQLLDLKAQLQQARSQQVSLAW
                     TYALGILVLACLAALAWLVLQQRRAGQEIQPKWHDSRELAVQVKPDPVPPPDTINAVV
                     APPIAANAAPEGIRPPAKPVAEIDINPDIFEHVVKAEPTVLPQSGATNFGNIHNISLE
                     PVLDIRQQAEFFVSLGQTERALQILKKQIASCTEPNPFVYLDLLELFHSLGLKADFRE
                     YRSTFNLYFTGAVPDFPAFHLEGDDLLAYPEVLARLVQGWPCAKTLVLLDDWIFRREQ
                     IPAQITFDLAAFRDLLMLHALAEEVATDLPWDTVSPEFQPDPVMRPKDKIASVMPTDV
                     RQPRASSLPKEMQDQSIDMDFSDLEIATGFLETSVIWPPEDAPSTRWPQTDRPG"
     gene            4718..5494
                     /locus_tag="PLB25_04325"
                     /pseudo
     CDS             4718..5494
                     /locus_tag="PLB25_04325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745598.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
     gene            complement(5587..6540)
                     /gene="gshB"
                     /locus_tag="PLB25_04330"
     CDS             complement(5587..6540)
                     /gene="gshB"
                     /locus_tag="PLB25_04330"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HOE40844.1"
                     /translation="MKLLFVADPIESFKIYKDTTFAMMREAQRRGHAVSVCEPRDLRW
                     VRGGLVEARARVISLTGEATEWYKIDSCSRSPIKGYDAVMMRKDPPFDAEYFYATHLL
                     EQAEREGATVFNKPRALRDHPEKLAIMEFPECIGPTLVTRDAQDIRDFHAEHQDIILK
                     PLDGMGGTGIFRVRQDGLNLGGIIETLNQDGAQTVMVQKFLPAIAQGDKRVLVIGGKP
                     VPYCLARIPQGGEVRGNLAAGGKGVAQALSARDREIAETIGPVLFERGLLLIGLDVIG
                     DSLTEINVTSPTCFQEIFDQTGFDVAAMFVQALEAAQALSH"
     gene            complement(6648..7862)
                     /locus_tag="PLB25_04335"
     CDS             complement(6648..7862)
                     /locus_tag="PLB25_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HOE40845.1"
                     /translation="MHFFKDLSPSAFTAGFVAVLVGFTSSVAIVFQAALAFGATPELI
                     TSWMWALGLGMGLCTLVPSLVLRKPVMVAWSTPGAAVLATAGVAGGFSMAEAVGAFMV
                     CAALITLAGATGWFERAMNHLPLAIASGLLAGVLARFGTQAFTAAQTNLPLVLLMLAS
                     YLLSRRWLARYAVPATLLIAIFYVAASAGFTGASVHFDLAMPVFTRPQFTWSAAISLA
                     LPLFIVTMASQNLPGVAVIHAAGYGHSAGKPGIPVSGIITLTGLVTLLLAPFGAFALN
                     FAAITAAICMGREAHPDPDRRYTAAVSCGLLYVLIGIFGTAITGTLTAFPKELIAAIA
                     GLALLGTIGSALATAVRDEFHREAALITFLVTLSGVVIAGVGSAFWGVIAGALALLVQ
                     RAGKSHFELPPR"
     gene            complement(8067..10322)
                     /locus_tag="PLB25_04340"
     CDS             complement(8067..10322)
                     /locus_tag="PLB25_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HOE40846.1"
                     /translation="MKSVVTVTMGSSDKDFEFKTSFLGQEFRVRRLGADQDTGKAWDL
                     MRRHQASADAIGLGEVTDHCHVGLRTVVNKETQRLLNVVTRVPVTTGATLRRLLQVRA
                     VRHVQKELGHYFNNNLVLFLSGMRNYDMAVAMSDYTHNLSFADALFQTGTPAMLTSLE
                     QLEIYAKGSEWMLSGKSGAVLESSLTQFKNQRVAGVVAKSHVIVGTFAEIKAVGNASN
                     LAGKTLITAAIDDERLAYFTACKVNLVIDVSPKLFEQVVAISTLEAMILAVLERPMEE
                     VSDDDFNEILTELDIKPRLLHPTGNFRNIRRFAFVIHPLSQDFIKKGFPIPKATPKFV
                     MDRVETLAAHMPPMVYCKMDNIISPTGAEAEGWLISVGGTPKEMLARSPEFTYRRLLH
                     AAKIAEKMGAQIMGLGAFTKVVGDAGITVARRSRLPITTGNSYSASGALWAATDAMRR
                     MGLVDINKKSQKVAAKSMVIGASGSIGSVSARLLAMAFEEVVIAGRDMKKLEELKKSI
                     LKDTPDANVVCSIDYDELLADMDMIVTSTSGAGKSILDITRVKPGCVITDVARPLDLP
                     AKDVAKRPDVLVIESGEIELPTEVHGMKSIGLPPNVIYACLAETIVLALEGRFEVFTV
                     GRDTEWEKVKEIYKLGRKHGMKLAAISGVNGVFTDEAIAKVVKLAKKARLTWKDGKSA
                     KPVKSVKPVKVAAEPSAKAKTPASKAKTRPLPSEIAATEAKPASVAASKKRAAKAVAK
                     KVPVEMPATLP"
     gene            complement(10319..11578)
                     /locus_tag="PLB25_04345"
     CDS             complement(10319..11578)
                     /locus_tag="PLB25_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOE40847.1"
                     /translation="MVATGLGAIMRTAGLFTRFIPIPQPTLLVGPGSSGRLGQAIAGF
                     GHSKILIVTDSIIAKLGLLKSLTDALTAGGAEFVVFDEITPDAPIPLIEKGILFYQAN
                     DCDAIVAFGGGSSMDASKAIAVAIANPKPLRQLAGYLKGLRTPVKIYAVPTTAGTGSE
                     VTVAAVISDPENHKKLVIVDPRMVPKMAALDPLLMTGLPPHITAATGIDALTHAIEAF
                     VGHWTTDYSDGMALSAVGLIFENLRTAYRNGKDIEAREKMSLASTYAGFAFTRANVGY
                     VHAIAHQFGGLYHTPHGLANAIMLPLVLKFSSPAITDRLALLAVSARVGTPDEPAEAL
                     AQKFLDAVDQLNADLGIPTTLEALQEADIPALAKAACREAHTGYPVPRYMSQQQCEEL
                     IGLALPQPHAKPAKRRSKKSTAGAAAK"
BASE COUNT         2401 a         3468 c         3397 g         2324 t
ORIGIN      
        1 ttcggcacga atgacaaagt tgatgccagc caaggtcgcc cgagtttcac tggatgccgt
       61 ttgccggagt ccgatggcaa cgaactcgct gcagcatcac tggcgctggc ttgaccggct
      121 tgtcactgat ctgaattttg gtcagcaagg ctgtttgcgc atgctcagcc gccgcagagt
      181 ccccagcgtg caccacggct agcggctcac cggcttgcac cttttgtccg atctgggcaa
      241 attggctaaa ccccactcgc gtatcaatcg tgtcgctgac cttgcgccgc ccaccaccga
      301 gttccacgat cagcagcccg atgtcgcggg ttgccatgct gctgacccag ccgccgtgcg
      361 gtgcttgcac caccctctgc accggcgcag ggggcaggta atggccaggg cgatcagcca
      421 ggtcagccgg gccgcccaag gctgaaatca tgcaagaaaa ccgctctagc gctcgcccac
      481 tggccaaagc atgatctact ttttgtagcg catcggtttc atcgctggcc agaccaccca
      541 tcagcagcag ctcggcactc agggtccgcg tcagcaacca ctggcgcggc tcgatggact
      601 ggcccttcag aaaagcgatg gcttccagca cctcgagcgc gttgccgcag gactggccca
      661 gcacctggtt catgtcggtc agccaggcac aagttggcag gccggcaccg tcggccacgc
      721 tcaccagtga ttcggccaac tcaccggcca tttccagtga atcggcaaag gctccgttgc
      781 ccgctttcac atccatgacc agcccctgca agccagccga gagttttttg gacaggatgc
      841 tggctgtgat cagcgacacc gattcgaccg tggcggtgac atcacgaatg gcgtagagcc
      901 gacggtctgc cggggccagc tcggcggttt ggccaatgat ggcgcagcca gcggtcttta
      961 gagtctggtg cagcatggcg acatcgggtg cagtctggta accggggatg cagcccagtt
     1021 tgtccagcgt gccgccggtg tgtccgaggc ctcttccgga gatcatcggt acaacgccac
     1081 cgcaggccgc cacgattggg gtcagcatca ggctgatctt gtcgccgaca cctccggtgg
     1141 agtgcttgtc cagaacgggt gcattgaagt tggccgttga ccagtccatc accagcccag
     1201 aatgggtcat ggcctgggtc aggttgaccg tctcattgcg gctcaagccg cgcaaaaaca
     1261 tcgccatggc gagcgcggcc gcctggccct cgctccacga gccatccacc aggccacgca
     1321 cgaagtgctc aatatcggca cgctccagtg tgtggccatc gcgtttgatg cgaatgatct
     1381 cttgcgcaag catggtgttg gcctagaagg gggtgtgaag caagactgct tgtggcgttg
     1441 tcgctttgcc ggggcaaatt caatagctac ccggcgcggc tgcgctggcg gggatgttgc
     1501 cagtcagcac cgcctcgatg tccgacagga tactgctggc accgatgcga aagcgtctgg
     1561 ggttgagtgc ctcgacaccc agtatctgcg ctgccagtga ttcataaaca atagcatcct
     1621 gcacagtccg gatgccgcct gacaccttaa aacccacatg acttctggcc acagggctgg
     1681 tcgcaattgc attgagcatg atgcgtgcag cctcgggtgt tgcgcttaat ggcgttttgc
     1741 cggtgctggt tttcaaaaag tcagcaccgg caaccagcgc aagttcactg gcacgctgaa
     1801 tcagtgccgg ggttcgcagt tcgccggtct ccaaaatgac tttgagcagc aggtttggac
     1861 aggcttggcg cacagcgctg atgcaggccg tcacagtcgc agcatcaccg gccatcagcg
     1921 ccctgaaggg cagcaccaca tcgacttctt gcgcaccggc ctggacgacc tgcaaggtgt
     1981 cttgcaccgc tgcgccgata tcggcattgc cgtgcggaaa attggccact gccgccacac
     2041 caatgtgcgc gggcagttgc gtgcgcgcga acgaggccag tcgtggccaa acacacactg
     2101 ctgccaccgg tccaaacggg ccttgtgcgc gtttgcaaat tctggcaatg tcagcctcgg
     2161 tgtcggcatc gttcaggctg gtaaggtcca ggcacgccaa ggcgatgcgg gcgctgcttt
     2221 cctggctgtg tgaccaggtg ggttggggcc gagaggtcga atgagttgtt gggttcatga
     2281 tgaccggtga tgatactttg cagcgttggt cagcccagcg gtcggcgtgt ttcaagatgt
     2341 gttttcttgg gtgtacgcgt tttcaatgta ggtttgatgc acgttcaatt cgattggaat
     2401 ccaaatcgca caagcctgtc gaatcaaggg agcatcgacg gtgaaccgac accgcagcag
     2461 cccatttgga atcggcgttc agccccacaa ctgggcgatg gacttgcaaa ttcggcacaa
     2521 actggcctcg ctggggtcgc ttttcgcagc tcggtccaca aggccaaaag gttcctagcc
     2581 aggtcgatca gtttgcggcc aacgggtgct aggcgcatct tctggcggcc atatcaccga
     2641 tgtttccaaa aagcccgtag ctatctccag atccgaaaag tccatgtcaa tagactggtc
     2701 ctgcatctct ttgggcaaac tcgaagctct gggttgccgt acgtctgtcg gcatcaccga
     2761 tgcgattttg tcctttggtc tcatcaccgg atcgggttgg aattcaggag aaaccgtgtc
     2821 ccacggcaga tcggtagcca cttcttcggc cagcgcatgc agcatcagca aatcgcgaaa
     2881 tgcggccagg tcaaatgtga tctgggctgg aatttgttca cggcgaaaaa tccagtcatc
     2941 cagcaaaacc agcgttttgg cgcagggcca gccctgaacc agccgggcca gcacttcagg
     3001 gtaagcgagc aaatcatcgc cctccaggtg aaacgccgga aaatccggta cggcaccagt
     3061 gaaatacaga ttgaaggtgc tgcggtattc acgaaaatca gccttcaggc ccagcgaatg
     3121 aaataactcc aacaaatcaa ggtatacgaa tggatttggc tcggtgcagg aagcaatctg
     3181 cttcttgaga atctgcaaag cccgctcggt ctgccccagc gacacaaaaa actcggcctg
     3241 ctggcgtatg tccagcacag gttccaggct gatgttgtgg atgttgccga aattggttgc
     3301 cccactttgg ggcaagacag tgggctccgc tttgaccaca tgttcgaaaa tatcggggtt
     3361 gatatcgatc tctgcgactg gcttggcggg tggcctgatg ccctcgggcg ctgcatttgc
     3421 agcgatcgga ggggccacta ctgcattgat cgtatcaggg ggcggcaccg gatcgggttt
     3481 gacctgaacc gccaactctc gcgaatcgtg ccattttggt tgaatctctt ggccagcacg
     3541 acgttgttgc aagaccagcc aggccagtgc tgccagacaa gccagcacca aaatgcccaa
     3601 ggcgtaagtc caggcaagcg aaacctgctg tgaccgcgcc tgttgcaact gggcctttaa
     3661 atcaagcagt tgagcgtcat ttttggcagc gatatcacgc aacgccttga tgtcggcttg
     3721 taaagaggca atttgctggg tttgcagcaa gacctctgcg gatggtccgg caagtttggc
     3781 cgactccaat gcgctgattc gggccgcgaa gtcatcgagc gggccaagtt tcaacacagc
     3841 tttaccggtt gccggttcgg gtgacttgcg cattacaacc ggtttgactg cctctgcgct
     3901 gaccgacgct gcgggttttg gcgcccctgc ctgcgcattg ctagcccgct cgggcaccgc
     3961 aaccctgcgc tgagcaactg tcttttgttt caccggggca gcatcgccag atggcgaacg
     4021 ctttggccga ccagatgcca attcgctttg ctcggaccca gcacgatgcg caaccgaccc
     4081 aacagtggcg atcttcgggg ccaaatcgcc cgactcaatg tcagtcaata aaacatagcg
     4141 tcttgttgca aaaaacccac aattgaagct cagatcgacg cttaccacag gttcatcaac
     4201 caccccggca gattcaattc tcaccacggc cgactctgct gttgccgatg tcactgttac
     4261 tttgggcttg ccctggcgtg catcagcgtt aaaaacgtcg gcagcaaaac aggacgcggc
     4321 caagtcatcc ccgctcgcgg cctggacctg aaccgagaca tcgagcgtgc gaccaataac
     4381 gaccgggcca cgcagttctc ccatggtcaa ggcatgcacg acgggcgcta ggctcaaaaa
     4441 aaggctcgat aacgcaactg caacaattcc agatcggctc ttcaaagcag ctttcataac
     4501 aaagtggtga ccaaaccagg cacgactggc gaaaaagacc gattgaatca agccgcgcgt
     4561 ggcaatcaaa tcagtcggaa aaaccttagc atacccggtg cgagcgccga ttttgttttt
     4621 tcttgccagg catcctttga gttttgtcac gaattcgtta agcttgtcgc cacgtgtcag
     4681 caaattgaca tcatcggaac aactggaaaa tcgccccatg tgccaattac ttggaatgaa
     4741 cagccacctg ccggccagcc tgaccttgag ttttacagga ttctcgcagc gtggcggcgg
     4801 cactgaccac catggcgatg gctggggaat cgcttttttt gaaagtgacg acagcacgcc
     4861 aggcaagggg gtgcggcatt ttgttgacaa acaaagtgct gccacctcac cgattgcgca
     4921 gatgctcaaa aactacccca tcaaaagcca caacgtggtg gcccatgttc gcaaagccac
     4981 ggtcggcctg gtcaccctgg aaaacaccca cccctttgtg cgcgaactgt ggggccgtta
     5041 ctgggtgttt gcccacaatg gcgacttgaa aaactacacg ccctacctgc acggccattt
     5101 caagccggtc ggcaacaccg acagcgaacg ggcgttttgc tggctgatgc aagagctatc
     5161 caagtcgcat gccagcgtgc catctgtgga cgaactgacc cgcaccctgc aggaactgat
     5221 tccgcagatc tcgcgacacg gcacctttaa ctttttgctc agtaacggcc aggcactgtg
     5281 ggcgcacgcc accacccatt tgtgctacgt gctgcgggcg catccgttta accaggtgca
     5341 cctgaaagac gacgatgtca cggtcaatct ggcgcagctc aatagccagg aggatcggct
     5401 ggccatcgtg gtgaccgagc cgctgaccac cgatgaaaac tggacggcct ttgcgccagg
     5461 cgaactcaac gtgtttgtcg atggcacact ggcgcattgc agttgccagt gcgccgcccc
     5521 gccgcgcccg gcacccatta ctgacccgtt cgggccggtc ccggtggtgg cggtcgggcc
     5581 tgcgcctcag tgcgagaggg cctgcgcggc ttcgagagcc tgtacaaaca tggctgccac
     5641 gtcaaacccg gtctgatcga agatttcctg aaaacaggtt gggctggtca cgttgatctc
     5701 ggtgaggctg tcaccaatca cgtcgagtcc aatcaacaac aaaccgcgct caaaaagaac
     5761 cgggccaatg gtttcggcga tttctcggtc acgcgcgctc aaggcttgcg ccacaccttt
     5821 gccgcccgcc gccagattgc cgcgcacctc accaccttgc gggatgcgcg ccaggcaata
     5881 gggcacaggc ttgccgccaa tcaccagcac gcgcttgtcg ccctgggcaa ttgctggcaa
     5941 aaacttctgc accatcacgg tctgggcacc gtcctgattg agcgtctcga tgatgccgcc
     6001 cagattcaga ccgtcttgcc tgacccggaa aatgccggtg ccgcccatgc catcaagcgg
     6061 cttcaagatg atgtcctgat gctcggcatg aaagtcgcga atgtcctggg catcgcgtgt
     6121 gaccagggtc gggccgatgc actcgggaaa ttccatgatg gccagctttt cggggtggtc
     6181 gcgcagggcc cggggtttgt tgaacactgt cgcaccttca cgctcggcct gctccagcaa
     6241 atgggtggcg tagaagtact cggcatcgaa cggcgggtct tttcgcatca taacagcgtc
     6301 gtagcccttg atggggctgc gtgagcagct atcaatttta taccattcgg tggcctcgcc
     6361 ggtgaggctg atgacgcggg cgcgggcctc aaccagaccg ccacgaaccc agcgcagatc
     6421 acgcggctca cacactgaca ccgcgtggcc gcggcgctgc gcctcgcgca tcatggcaaa
     6481 agtggtgtcc ttgtaaatct tgaacgattc aatcgggtcg gctacaaaca acagcttcat
     6541 ggggcaatca ctttcagggc tcaatctttt gcaatgcggg gcatttcgcc atcgcggcta
     6601 tgacgggggt cattcatcat ttttttgccg ctgcaatgcc ttgcgcgtta gcgtggcggc
     6661 agctcaaaat gagattttcc ggctctttgt accagcaacg ccaaggcacc ggcaatcacc
     6721 ccccaaaacg ccgagccaac accggcgatc acgacaccgc tgagggtcac cagaaaggtg
     6781 atgagggctg cctcgcgatg aaactcgtca cgcaccgcag tggccaacgc cgagccaatt
     6841 gtgcccagca gcgccagacc ggcaatggcg gcaatcagct ctttgggaaa ggcggtcagc
     6901 gtgccggtga tggcagtgcc aaaaatgcca atcaacacat acagcaggcc acaggagacg
     6961 gcagcggtgt agcgtcggtc agggtccggg tgcgcctcgc ggcccatgca gatggcagcg
     7021 gtaatggcag cgaaattcaa tgcaaacgcg ccgaacgggg ccagcagcaa cgtcaccagg
     7081 ccggtcagcg tgatgatgcc tgacaccgga atgccgggtt tgccagcgct gtggccatag
     7141 cccgcggcgt gaatcaccgc cacccccggc aagttctgcg aagccatggt cacgataaac
     7201 agcggcagcg ccaggctgat cgccgcgctc caggtgaact ggggccgggt gaacaccggc
     7261 attgcaaggt caaaatgcac gctagccccc gtgaatcctg cgctggcagc tacataaaag
     7321 atagcaatca acaaagttgc tggcactgcg taacgcgcca gccagcgccg actcagcaag
     7381 taactggcga gcatcagcaa caccagcggc aaattggtct gcgcagcagt aaaagcctgc
     7441 gtgccaaaac gtgccagcac gccagccagc agacctgacg caatggccag gggcagatgg
     7501 ttcatggcgc gctcaaacca gccagtggcc cctgccaacg tgatcaacgc agcacacacc
     7561 atgaaggcac cgaccgcctc tgccatcgaa aagccgccgg ccacgccggc agtggccagc
     7621 accgccgctc ctggggtgct ccaggccacc attacaggct tgcgcagcac cagcgagggc
     7681 accagcgtac acagccccat gcccaggccc agcgcccaca tccacgaggt gatcagctcg
     7741 ggtgtggcac caaaggccag cgccgcctga aacacgattg ccaccgaact ggtaaagccc
     7801 accaacacgg caacaaaacc ggcggtgaag gcagacgggg acaggtcttt gaaaaagtgc
     7861 atggggctga ttttccccca ctcctcgccc aggcatgccg ccgcgccccg gaccgagctt
     7921 gtcagcacaa tacaggcagc tcaaaggtat caaaaaagct attatttata tagctagtac
     7981 cgctacatcc ataagggcta caaggcgaaa aggcatataa gatcggggat tgccgctggc
     8041 taccgcagtg gcccggcacc cctgatctac ggcagcgtgg ccggcatttc gaccggcact
     8101 tttttggcca cggccttggc ggcgcgcttt ttgctggcgg cgacgctggc gggctttgct
     8161 tcggttgcag cgatctctga cggcaggggt ctggtttttg ccttgcttgc cggtgtcttt
     8221 gctttggcgg atggttcagc ggcgaccttg accggcttga ccgacttgac cggtttggcc
     8281 gacttgccat ctttccaggt caggcgtgct ttcttggcca gcttcaccac cttggcaatg
     8341 gcttcgtcgg taaatacccc gttgacaccg gaaatggcgg ccagcttcat gccatgcttg
     8401 cgaccgagct tgtagatttc cttgaccttt tcccattcgg tgtcgcgccc gaccgtgaac
     8461 acctcgaaac ggccttcgag cgccagcaca atggtctcag ccaggcaggc atagatcacg
     8521 ttgggcggca ggccgatgct cttcatgccg tggacttcgg tcggcaactc gatctcgccg
     8581 gattcgatga cgagcacatc cgggcgtttg gcgacatcct tggcgggcag gtccagtgga
     8641 cgcgccacgt cggtgatgac gcagccgggc ttgaccctgg tgatgtccag aatcgacttg
     8701 ccggcacccg aggtgctggt gacgatcatg tccatgtcgg ccagcaactc gtcgtaatcg
     8761 atcgagcaca ccacgttggc atcaggcgtg tctttgagga tggatttttt cagctcttcg
     8821 agctttttca tgtcgcgccc ggcaatcacg acttcttcaa aagccattgc cagcaggcgt
     8881 gcgctgaccg aaccaatcga gccgctggca ccaatcacca tgctcttggc cgcgactttt
     8941 tggctctttt tgttgatatc caccaggccc atgcggcgca tcgcatcagt ggcagcccac
     9001 agtgcaccgg atgccgagta gctgttgccg gtggtgatgg gcaatcggga gcggcgcgcc
     9061 acggtaatgc cggcgtcgcc caccaccttg gtgaaagcgc ccaggcccat gatctgggcg
     9121 cccatctttt cggcaatttt ggcggcatgc agcaagcgcc ggtaggtgaa ctcagggctg
     9181 cgcgcgagca tttctttcgg ggtgccaccg accgagatca gccagccttc agcctcggca
     9241 ccggtaggcg agatgatgtt gtccatcttg caatagacca tcggcggcat gtgggccgcc
     9301 agggtttcca cccggtccat gacaaacttg ggcgttgcct tggggattgg aaagcccttc
     9361 ttgatgaagt cctggctcag gggatgaatc acaaaggcaa aacggcggat attgcggaag
     9421 ttgccggtgg ggtgcagcag gcgcggtttg atgtcgagtt cggtcagtat ttcgttgaaa
     9481 tcatcgtcgg acacctcttc catgggccgc tccagcaccg ccagaatcat cgcctcaagc
     9541 gtgctgatcg ccaccacctg ctcaaacagc ttgggcgata catcgatcac caggttcacc
     9601 ttgcaggcgg tgaaataggc caggcgctca tcgtcaatgg ccgccgtgat cagggtcttg
     9661 ccagccagat tgctggcatt gccgaccgcc ttgatctcgg caaaagtgcc aacgatgacg
     9721 tgcgacttgg ccaccacgcc agcgacccgc tggttcttga actgggtcag cgaggactcc
     9781 agcaccgcac cgcttttgcc cgacagcatc cattcactgc ctttggcata gatttccaac
     9841 tgctccagcg aggtcagcat ggcaggtgtg ccggtctgaa aaagtgcgtc ggcaaaactc
     9901 aggttgtggg tgtagtcgga catggccacc gccatgtcgt agttgcgcat gcccgacagg
     9961 aacaagacca ggttgttgtt gaaataatgg ccgagttcct tttgcacatg acgcaccgcg
    10021 cgcacctgca gcaggcgacg cagcgtggct ccggtggtca ctggcacccg ggtcaccaca
    10081 ttgagcaggc gctgggtttc cttgttgacc acggtacgca agccgacatg gcaatggtca
    10141 gtgacctcgc ccaagccaat ggcatcggca ctggcctggt ggcgacgcat caggtcccag
    10201 gctttgccgg tatcctgatc ggcacccagg cgacgaaccc tgaattcctg ccccaaaaaa
    10261 ctggttttga actcgaaatc cttgtcggat gagcccatgg tgacggtgac gacgcttttc
    10321 attttgctgc tgctcctgcg gttgattttt tgctccggcg cttggctggt ttggcgtggg
    10381 gttggggcag tgccagccca atcagctctt cacattgttg ctgcgacatg taacgcggca
    10441 ccgggtagcc ggtatgcgcc tcgcggcagg ccgctttggc cagcgcgggg atgtcggcct
    10501 cttgcaaggc ttccagcgtg gttgggatgc ccagatcggc attgagctgg tcgaccgcgt
    10561 ccagaaattt ttgcgccagc gcttctgccg gttcatctgg cgtgccaacc cgggcactga
    10621 ccgccagcag cgccagccgg tcggtgatgg ccgggctgga aaatttgagc accagcggca
    10681 gcatgatggc attggccagg ccgtgcgggg tgtggtaaag cccgccaaac tgatgggcaa
    10741 tggcatgcac atacccgaca ttggcgcgcg tgaaggcaaa accggcgtag gtggaggcca
    10801 gcgacatctt ctcgcgggct tcaatatctt tgccgttgcg gtatgcggtg cgcaggttct
    10861 cgaaaatcaa tccaacggct gacaaggcca tgccgtcgga ataatcggtg gtccagtggc
    10921 cgacaaaggc ctcaatcgcg tgggtcaacg cgtcaatacc ggtggcggcc gtgatgtgcg
    10981 gcggcagacc ggtcatcagc agggggtcga gcgccgccat tttgggcacc atgcgcgggt
    11041 ccacgatgac cagcttcttg tggttttccg ggtcggagat gacagcggct accgtgactt
    11101 cggagccggt gccagccgtg gtgggcacgg catagatttt gacgggcgtg cgcaggccct
    11161 tcaaataacc ggccaactga cgcaacggct tggggttggc aatggccacc gcaatcgcct
    11221 tggaggcatc catgctggag ccgccgccaa aggcaacgat ggcatcgcag tcgttggcct
    11281 ggtaaaacaa aatgcccttt tcgatcaggg gaatcggcgc atccggggtg atttcgtcaa
    11341 acaccacaaa ctcggcaccg cccgccgtca gggcatcggt cagacttttg agcaggccca
    11401 gcttggcgat gatgctgtcc gtgacgatca gaattttgct gtggccaaat ccggcaatcg
    11461 cctgacccag gcggccactg gaaccaggcc cgaccagcag ggtcggctgc ggaatgggga
    11521 tgaaccgggt gaataacccg gctgtgcgca tgatggcacc aagaccggtg gcaaccagcg
    11581 cgttgcggtt
//
LOCUS       DAOSLL010000065        11538 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5567_length_11538_cov_4.215362, whole genome shotgun sequence.
ACCESSION   DAOSLL010000065 DAOSLL010000000
VERSION     DAOSLL010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11538
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5567_length_11538_cov_4.215362"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="PLB25_04350"
     CDS             <1..465
                     /locus_tag="PLB25_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40848.1"
                     /translation="GAATQALLGGTWTHQSRFSVLAEAWWDGTAPSDAQWRNWFARNQ
                     LLSDLAAMGAPASAVAGNLAWQTEALSTTGSLRRSNIYLRLSWEHENWPPTLDLLYHP
                     ADGGRMWTAALLYKGDRVRLQGGLRISTGGADAVLGQLPTGRQAYLSGTWTF"
     gene            complement(641..1705)
                     /locus_tag="PLB25_04355"
     CDS             complement(641..1705)
                     /locus_tag="PLB25_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40849.1"
                     /translation="MTHLNPIELAQRYFVNDCPEATILASRLGNILDKLQQGHQISSI
                     ALGYLHKQGFFSLERLIQGEITYPQFCADAQAEQAQRVMLAQAQREAKIAEEAAREAA
                     WAARYALERQRAEQARIARESDPRYIKKKHDQQLRVRYGIDQFIEKDCFGRLMDILHR
                     VDGANRFTDEDVVWLKTKGRDYYSDTLKNVFHQREAKFFASEYQRTHDAWMAVNASKH
                     YRKCDQAQSAHDLLAPIALEQQSSAKLKSALCTTYGGAMRDLGQHGPALQLGQRAHAL
                     MPKDFRPCTLLGALHIEMGNYQLGQEWYAKAQERGASKQAIDQELRGIFQRADKSNRE
                     EIKAFLLGQDPVRYKWVNVA"
     gene            complement(1718..2863)
                     /locus_tag="PLB25_04360"
     CDS             complement(1718..2863)
                     /locus_tag="PLB25_04360"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE40850.1"
                     /translation="MFPVVALLGPRQVGKTTLARQMAAQWPGLTHHFDLEDPDDQARL
                     SDPAFVLRRLTGLVVLDEIGLQPALFPLLRVLADRQDTPARFLILGSAAPELLRNTSE
                     SLASRVTFHELDGLALDELAPEQLDERWLRGGFPRSFLAPDTLASRAWRESFVRTYLE
                     RDLPQLGINLPALTLRRFWTMLGHYHAQTWNGSELARALGVSDKTVSRYLDILEGTFM
                     TWRLRPWHTNQGKREVKAPKVYLADTGLLHTLLGIGSEQDLLAHPKCGASWEGFALHE
                     VIRQSGAGRDEAYFWALHSGAELDLLIVRNGRRFGFEVKLTRSPKITPSMRSAQQALD
                     LEHLYVVCHGDGQVMPLAEGISSVPLQALASLNLSNVRSPHRTLIHP"
     gene            complement(3483..3743)
                     /locus_tag="PLB25_04365"
     CDS             complement(3483..3743)
                     /locus_tag="PLB25_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2442 domain-containing protein"
                     /protein_id="HOE40851.1"
                     /translation="MFKAEPKATTVVCSNDELRVSLTDGRTLTVPMAWFPRLVHASSD
                     ERADYELLGDGEGIHWPQVDEDISVAELLAGQPSVEARGAFA"
     gene            complement(3770..4021)
                     /locus_tag="PLB25_04370"
     CDS             complement(3770..4021)
                     /locus_tag="PLB25_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HOE40852.1"
                     /translation="MRKEYDFSQGKRGAVLPSTGKTRVTIMLDDDVIEAFRARAEASG
                     RGYQTMMNEALRAAMDPDAAPVTLRTLREVLDERFHLVA"
     gene            complement(4014..4298)
                     /locus_tag="PLB25_04375"
     CDS             complement(4014..4298)
                     /locus_tag="PLB25_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019922786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HOE40853.1"
                     /translation="MHNPAMQFEFDPAKARSNLQKHPGVSFSHAEQSLRDDMAVTIED
                     PDVVGEQRFLTLGMDALGRILVVCHTQRDERTRIISARKASKNEAKNYYA"
     gene            complement(4324..4488)
                     /locus_tag="PLB25_04380"
     CDS             complement(4324..4488)
                     /locus_tag="PLB25_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40854.1"
                     /translation="MGQKHGLQFDVKTGVQRSIVAEIEAEQALVAANRELIRRMEAKV
                     KATIDRVWGN"
     gene            complement(4455..5051)
                     /locus_tag="PLB25_04385"
     CDS             complement(4455..5051)
                     /locus_tag="PLB25_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HOE40855.1"
                     /translation="MTVVASIVEGDGEVAALPVLLRRLAQWRGAAGHVNVLPPIRVYK
                     DRFLNRPEEFSRHLKLAAAKCGDAGWILVLFDADDDCPAQKGVAVLAQAQAIIPHRRM
                     AVVLANREYEAWFIAAAESLNGCRGFQSHANDAQLDPEIPRNAKGWVRERMPAGYGET
                     TDQPAFSARFDLDLAHQRSRSFRKLCSEWDKNMVSNLT"
     gene            complement(5048..6253)
                     /locus_tag="PLB25_04390"
     CDS             complement(5048..6253)
                     /locus_tag="PLB25_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE40856.1"
                     /translation="MTTPVFLTRVLLRNYKSIGACDVRLGPLTYLVGANGSGKSNFLD
                     ALHLVRDALAGSLDNALNERGGLMEVRRRSSGHPTNFAIRLEFILRDGQIGYYSFNVG
                     ALAGRGYEVQAEKCVIGGKGKGPYFHVERGVLKDSSEPTFPAVTADRLALVSASGMTV
                     FRPVFDALTGMGFYNLNPKLMRDPQKPQDGRMLKPVGENIVSVIGHLGRVAPAQLEQI
                     ESYLQTVAPMIRGLVRKPIGPLETLEFRQEVAGAKAPWHFPAQNMSDGTLRALGVLTA
                     LFQGNPDHAPSLIGIEEPETALHPAASAALREALTKASASTQIMVTSHSPDLLDDHSI
                     EPEHILAVVSERGDTRIGPLDAASVSVMRNHLFSAGELLRLNQLQPNSDFLSRQSTAQ
                     VDLFGEMVR"
     gene            complement(6250..8802)
                     /locus_tag="PLB25_04395"
     CDS             complement(6250..8802)
                     /locus_tag="PLB25_04395"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOE40857.1"
                     /translation="MLDSETKRRIDACRDILVGKVPDPKSQVEQITIALIYKFMDDMD
                     AEAEELGGKRSFFAGSYARFGWAHLLAPGLGGFEVLALYSEAIASMRQNPGLPELFRD
                     IFKNAYLPYRDPETLKSFLKEINVFTYDHSEKLGDAFEYLLSVLGSQGDAGQFRTPRH
                     IIDFMVTVLDPKKNETILDPACGTAGFLISAWKHILAANTPANDVATEQASHSENNSY
                     SGNQDGGYKLKSLTPDERIRLAANIRGYDISPDMVRLSLVNLYLHGFSDPHVVEYDTL
                     TSDEKWNEVFDVILANPPFMSPKGGIKPHKRFSVKASRSEVLFVDYMAEHLTATGRAA
                     IVVPEGIIFQSGTAYKALRKMLVDTSLVAVVSLPAGVFNPYSGVKTSILILDKRVHKQ
                     TDKVLFVKVQNDGFNLGAQRRAVMDSHLPEATRLLRGWMVAPGKLEGDGVMAQAVARE
                     RIGLSGDLNLSGERYVVASAQAHAWPVVMLGEIVDELESGVSVNSENRNVVGDELGIL
                     KTSCVTTGIFDPQEHKAVLPEELPRVRCPVRKNSIIISRMNTEALVGANAYVDKDFDH
                     LYLPDRLWQTVITRPDVDVRFVQFILASKECREIISSKCGGTSGSMKNIAKPQLLSLQ
                     IPLPPREVQHQIVAEIEGYQKIIDGARQVLDNYQPGIVVQANWPLVELGKLCSVGGTI
                     TTDVDLSLPYFGADSIESNSGRLLKTETAQSQRVNGPVYEFSGERLRYSKIRPYLNKL
                     TIVDLCGYCSSDMYPLVPASSQIEIQYLATYMLSKVFNDGIRGHYERASIPKINRAQL
                     FAAAIPLPDLETQRTIVAEIEAEQALVAANRELIRRFEAKIKTTIDRVWSAS"
     gene            complement(8867..9046)
                     /locus_tag="PLB25_04400"
     CDS             complement(8867..9046)
                     /locus_tag="PLB25_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40858.1"
                     /translation="MKQTSFTKTPITSSAPRDDAPKLTQADFDQAKLRLGGRDVNQAE
                     WQIAVRRVIREELSH"
     gene            complement(9130..10524)
                     /locus_tag="PLB25_04405"
     CDS             complement(9130..10524)
                     /locus_tag="PLB25_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40859.1"
                     /translation="MINPLVVDARTSVTTQLLSDQGYAAVVIADTAGGELTSEQVFKQ
                     RNFEKQFFSEPTNRLFCETLLKHGLCDPISNEFGKTIVFAVSQAHASKITQVLNELSD
                     QIYPGRYQSDFAVQVTSLIPDAQQFTINFTDKSNNLMGTGNAMPQYKTSKARICVTVG
                     MMTTGYDCPDLLNLALMRPVFSPSDFVQIKGRGTRKHDFREQLQDETLKTQVTAPHKT
                     AYKLFDFFATCEYFEEKFNYDEVLKLPRPGTGGEDPSPPPPPPPPGHPFEHTGPDRIT
                     SQVEQQVGFQGMKVDRMFFEKFEDKVKADPVIAAQIQDEQWDQAINYITTELFDKPAE
                     YFTLDKLRRAAGVDRRLGLKEILQKTFGLITGFKSKDELLEDEFDKFLLDQQNATGFQ
                     ADSTATAVQAMKYYFKAYATDHRLRDIIEAQRLTDLNVYPAFGMSDFKAVPRVWRTRI
                     PEYVKDYVPLNQFM"
     gene            complement(10521..>11538)
                     /locus_tag="PLB25_04410"
     CDS             complement(10521..>11538)
                     /locus_tag="PLB25_04410"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOE40860.1"
                     /translation="TTSHGFIDFLLLDEQSFPLVVLEAKAEDKNPLIGKEQARRYAKS
                     QACRFVILSNGNLHYFWDLSHGNPHVITAFPAPASIKGYAKFQPDPARLTTEDVPLDY
                     IARTQLPTYDLEAGWNHQIARDAFIQKNRLRFLRLYQQRAARTVQAAVKAGQTRFLFE
                     MATGTGKTLTAAAVIKLFLRTGNARRVLFLVDRLELEDQANKAFIALLKNDYKSVIYK
                     ESRDDWRKAEIVVTTVQSLLFNAKYKQFSPTDFDLVISDEAHRSIGGNARAVFEYFVG
                     YKLGLTATPKDYLKSLMQPGPVPATRASQNAACCSTPTAPSAAKVASPPTAIRCWTAS
                     KTVF"
BASE COUNT         2318 a         3242 c         3366 g         2612 t
ORIGIN      
        1 ggtgcggcca cacaggcgct gctgggtggc acctggacgc atcaaagccg gttcagcgtg
       61 ctggccgaag cctggtggga cggcacggca ccttcagacg cgcagtggcg caactggttt
      121 gcccgcaacc aactgttgtc agacctggca gccatgggcg cgccagccag cgccgtggcc
      181 ggcaacctgg cctggcagac agaagccctg agcacgaccg gcagcctgcg gcgcagcaac
      241 atttacctgc gcctgagctg ggagcacgag aactggccac ccacgctgga cctgctgtat
      301 cacccggcag acggtggacg catgtggacg gcggcactgc tgtacaaggg cgacagggtg
      361 cggctgcagg gtggattgcg catcagcacg ggtggggcgg acgcggtgct ggggcagttg
      421 ccgactggca gacaggctta tctgagtggg acttggacat tttgagccgt gtcgctggct
      481 gggcaggacc aaaagttcat acaaaagtgc ctctagccct tgttccaatt gcgctggtag
      541 ctactcaatc gagagtagcg atgaatctgc aattgtggct gattcactgt atgccaagtg
      601 tcacggtcgc agtctcgcag cttgcccaag atgctgcggc tcaggcgaca ttgacccatt
      661 tgtatcgaac cgggtcttga cccagcaaga atgccttgat ctcctcgcga ttcgacttgt
      721 cggcacgctg gaaaatgccg cgcagttcct ggtcaatggc ttgcttgctg gcaccacgct
      781 cctgcgcttt tgcataccac tcttgaccaa gttggtagtt tcccatttcg atatgcaacg
      841 ccccgagcaa tgtgcagggt cgaaagtcct ttggcatcag ggcatgagcg cgctggccga
      901 gctgtaaagc tggcccgtgc tgcccaaggt cgcgcatcgc gccgccgtaa gtcgtgcaga
      961 gcgccgactt caatttcgcg gaactttgct gctcaagcgc gattggggcg agcaggtcat
     1021 gggctgattg ggcctgatcg cacttgcggt agtgtttgct cgcattgacc gccatccagg
     1081 catcatgggt gcgctggtat tcgctggcga agaacttggc ttcgcgttga tgaaaaacgt
     1141 ttttgagcgt gtctgaatag taatcacgac ccttggtttt cagccacacg acatcttcgt
     1201 cggtgaagcg gtttgcgcca tcaacccggt gaaggatgtc catcaagcgg ccaaagcaat
     1261 ccttttcgat gaactggtca atcccataac gaacccgaag ttgctgatcg tgcttttttt
     1321 tgatgtacct cgggtcgctc tctcgcgcga tgcgggcttg ttcggcccgc tgacgctcaa
     1381 gcgcataccg tgcagcccag gctgcctcgc gcgccgcctc ttcagcaatt ttggcctccc
     1441 gctgcgcttg ggccagcatc acgcgctggg cctgttcggc ctgagcatcg gcgcaaaact
     1501 gcggataggt gatttcgccc tgaatcaagc gttcaagcga gaaaaaccct tgcttgtgca
     1561 gataacccag cgcaatggac gaaatctggt ggccctgctg aagcttgtcg agaatgttgc
     1621 ccagacggct ggcgaggatg gttgcctcgg gacagtcgtt gacgaagtaa cgctgtgcca
     1681 gctcaatcgg attcaaatgt gtcatggttt gtatgggtca cggatggatc agcgtccggt
     1741 gtggtgatcg tacgtttgac agattcaggc tggcaagtgc ttgcaagggt accgaactga
     1801 tgccttcggc cagcggcatc acttggccgt caccatggca gaccacatac agatgctcca
     1861 gatccaaagc ctgttgcgcc gagcgcatgg acggcgtgat ttttggtgag cgggtgagtt
     1921 tgacctcaaa cccgaatcgg cgaccgttgc gcacaatcag cagatccagt tcagcccctg
     1981 aatgaagcgc ccagaaatag gcctcatcac gcccggcacc gctttgccga atcacttcat
     2041 gcagcgcaaa gccttcccag gatgcgccac attttggatg cgccagcagg tcctgctcgc
     2101 tgccgatgcc cagcaaagtg tgcagcaagc cggtgtccgc caggtaaacc ttgggtgcct
     2161 tgacttcacg ctttccttgg ttggtgtgcc aggggcgtag ccgccaggtc atgaatgtgc
     2221 cttccagaat atccagataa cgtgacaccg ttttgtcgct tacccccagg gcgcgcgcca
     2281 attcgctgcc gttccaggtt tgcgcgtggt aatgcccgag catggtccaa aagcgacgca
     2341 gtgtcagggc cggcaagttg atgccaagtt gcggcaaatc gcgctcaagg taagtgcgga
     2401 caaacgactc gcgccaagcc cggctggcca gcgtgtcggg ggccaaaaaa gaccgtggaa
     2461 agcccccacg cagccagcgc tcgtcaagtt gttcgggtgc cagctcgtct aacgccaagc
     2521 catcgagctc atgaaaagtc accctgcttg ccagactctc ggaagtgttg cgcagcaatt
     2581 caggggccgc gctgcccaag atcagaaaac gggctggcgt atcttgccgg tcagccagca
     2641 cacgaagcag cgggaacaac gctggctgaa gcccgatttc atcgagcacc accagcccgg
     2701 tgagccgacg cagcacaaac gccgggtcac tcaggcgcgc ctggtcatcg gggtcttcca
     2761 gatcaaaatg gtgcgtcagt ccgggccatt gcgctgccat ttggcgcgcc aaagtggttt
     2821 tgccaacctg gcgcgggcca agcagggcca cgaccgggaa catcgccaag tctttcgata
     2881 tttgctgaag atggcatcgt cgttcaatca ttttgtcatc gagccatgaa aattcggagt
     2941 gtatatccga aatttcgcag tgaaaagcga ttgccaccca ttcatcagcg tatcgctcag
     3001 ggtacgggca cggctcatgg cgcaacgtca tgcacgactc ggcggggccg gggcgacttt
     3061 atagattttg gagagtaaag agagcataag gggaaggctc ggatcgtgct cggttttgcc
     3121 cgctacggcg cacacaccgg aagcagcctg ggtggccagc gacgcgctcg agttgcatgc
     3181 ttcggtgctt gccatgcggg acaccgacgc caatgctttt gtaattgggc agtaccacgc
     3241 gggtcagaaa aacaggggtg gtcatgcggc gctccagaca cggtcgatgg tggttttgat
     3301 tttcgcctca aagcagcgga tgagttcgcg gttggcggcc accagcgctt gctcggcttg
     3361 gttctcggcg acgagggaaa gttggatgaa cagaccaaca cggctccttt caactttgct
     3421 gcttcggcag caaagttgct taggggtttt tctttggcgc ttgcaaatgc gccttcattc
     3481 gctcaggcga acgcgcctct cgcctcgaca gaaggctgtc ctgccaaaag ctcagccact
     3541 gaaatgtctt catcaacttg cggccagtga atgccttcgc cgtcgcccaa caattcgtag
     3601 tcggcacgct catcgcttga tgcatgcacc aaccgaggga accaagccat agggactgtc
     3661 agcgttcgcc catctgtgag cgacacgcgc aattcgtcgt ttgagcaaac gacggtcgtc
     3721 gctttgggct cggctttaaa catgaaagaa cgcattccag gcctctggct catgccacca
     3781 gatgaaaccg ttcatccaat acttcgcgca aggtgcgcaa ggtgacaggt gctgcatcgg
     3841 ggtccatggc ggcgcgcaaa gcttcgttca tcatggtttg ataaccccgg ccagaggcct
     3901 cggcgcgcgc acggaaggcc tcgatcacgt cgtcatcaag catgatggtg acgcgcgtct
     3961 tgccagttga aggcagcacc gcgccacgtt taccctggct gaaatcgtat tctttacgca
     4021 tagtaatttt tagcctcgtt tttactggct ttgcgagcag aaataatgcg cgtccgctca
     4081 tcacgctggg tgtggcacac caccaaaatc cgccccaatg catccatacc cagcgtcaaa
     4141 aaccgctgtt cgcctaccac atcagggtct tcaatcgtta ccgccatgtc gtcgcgcaaa
     4201 gattgttcgg catgagaaaa actgaccccc ggatgctttt gcagattgct gcgcgccttt
     4261 gctgggtcga attcaaactg catggctgga ttatgcatat taaatgcata tcgtcaaatc
     4321 ggctcagttc ccccacaccc ggtcaatggt ggccttgacc ttggcttcca tgcggcggat
     4381 gagctcgcgg ttggcggcta ccagggcttg ctcggcttcg atttcggcga cgatgctgcg
     4441 ttggacaccg gtttttacgt caaattggag accatgtttt tgtcccattc tgaacaaagc
     4501 tttctgaatg accggctgcg ttgatgtgcc aaatctaaat caaatcgtgc cgagaacgca
     4561 ggttgatctg tggtttcacc gtagcccgct ggcatgcgct cgcgcaccca accttttgca
     4621 tttcgcggta tttcagggtc aagctgcgca tcattggcgt ggctctggaa gccccggcaa
     4681 ccgttcaatg actcggcagc agcaataaac caggcctcgt attcccggtt ggctagcacc
     4741 acagccatgc gccgatgagg aatgatggcc tgcgcttgtg ccaacaccgc cacccctttt
     4801 tgagcagggc aatcatcatc ggcatcaaac aaaaccaata tccaacccgc atcaccacat
     4861 ttggccgcag ctaacttcag atgccttgaa aactcttccg gacggttcaa gaaacggtct
     4921 ttgtataccc gtatcggcgg caaaacattc acatgacctg cagcgccgcg ccattgcgcc
     4981 agccgtcgca acaaaacggg tagtgcggca acttctccat caccttccac aatcgaggcc
     5041 accaccgtca tcgcaccatt tccccaaaaa ggtcaacttg cgcggtagat tgcctgctca
     5101 aaaagtcgct gttgggctgc aactgattga gtcgcaacag ctcacctgct gaaaacagat
     5161 ggttccgcat cacactgaca gaggccgcgt ccagcgggcc aatccgcgtg tcaccacgct
     5221 cagacaccac ggccaaaatg tgctcgggct caatgctgtg atcgtccagt aaatcggggc
     5281 tgtggctggt caccataatc tgggtcgatg ccgaggcctt ggtcagtgct tcgcgcaatg
     5341 cggcgctggc tgcaggatgc agtgccgttt caggttcttc aatgccgatc aacgacggcg
     5401 cgtgatccgg gttcccctga aacaaagcgg tcaacacgcc cagagcacgc agcgtgccgt
     5461 cagacatgtt ttgcgcagga aagtgccatg gcgcttttgc cccggcgact tcctggcgga
     5521 actccagagt ctccaatggc ccaattggct tgcgcaccag gccgcgaatc attggtgcta
     5581 cggtttgcag atagctttca atctgctcca attgcgctgg cgctacgcgc cccagatggc
     5641 caatgacact gacaatgttc tcacccaccg gcttgagcat gcggccgtct tgcggctttt
     5701 gcgggtcgcg catgagtttg ggattcaggt tgtaaaaccc catgcccgtc aatgcgtcaa
     5761 acacggggcg aaacaccgtc atccccgatg cgctgaccaa ggccaaccgg tctgcggtca
     5821 cggcaggaaa agtgggctca ctgctgtctt tgagcacacc acgctcgaca tggaaataag
     5881 ggcccttgcc cttaccgcca atcacacatt tctctgcctg aacctcatat ccccgacctg
     5941 ccagcgcgcc cacattaaag ctgtagtaac caatttgacc atcgcgcaga atgaattcca
     6001 gccggattgc aaaattggtt gggtgcccgc tggagcgcct gcgcacctcc atcagtccgc
     6061 cgcgttcgtt gagtgcgttg tccagagagc ccgccaatgc gtcgcggaca aggtgcagtg
     6121 catccagaaa attgcttttg cccgatccgt tggcgcccac caaatacgtc aatggaccca
     6181 ggcggacatc acaggcgcca atgcttttgt aattgcgcaa taacacgcgg gtcagaaaaa
     6241 caggggttgt catgaggcgc tccagacacg gtcaatggtg gttttgattt tcgcctcaaa
     6301 gcggcggatg agttcgcggt tggcggcgac cagcgcttgc tcggcttcga tctcggcgac
     6361 gatggtgcgt tgggtttcga ggtcggggag ggggattgcc gctgcgaaca actgcgcccg
     6421 attgatcttg ggaatgctcg cacgttcata gtgcccgcgt atgccatcgt taaaaacctt
     6481 ggaaagcatg taggtcgcca aatactgaat ttcaatctgg gacgaggcgg gaactagcgg
     6541 gtacatatcg gagctgcaat agccacacag gtcaacgatt gtcagtttgt tgagataggg
     6601 gcgaatcttg ctgtaccgca atcgttcccc agaaaactca taaaccggac catttacccg
     6661 ttggctctga gctgtctcgg ttttcaatag cctgccactg tttgattcaa tactgtcagc
     6721 tccgaagtat gggagcgata aatctacgtc cgtagtgatc gtgccgccta cagagcacag
     6781 ttttccaagt tctacgagcg gccagttagc ctgcaccacg atgccaggtt ggtagttgtc
     6841 gagcacctgc cgggcgccgt cgatgatttt ctgataaccc tcgatttcgg ccacgatttg
     6901 gtgttggact tcgcggggcg ggagggggat ttgtagagaa agaagttgag gctttgcgat
     6961 atttttcatg ctgccgcttg tgccgccaca cttggaactg atgatttccc ggcattcttt
     7021 agacgcaaga atgaactgga caaagcgcac atctacgtct ggtctggtga tcactgtttg
     7081 ccaaagtcgg tcaggcaaat agagatgatc gaaatctttg tccacatatg catttgcccc
     7141 caccagcgct tctgtgttca ttctgctgat gatgattgag ttttttctta ctgggcatcg
     7201 aacccttggc agttcctcgg gcaaaacagc tttgtgctct tgtgggtcga agatgccagt
     7261 ggtcacgcag cttgttttga gaatgcccag ctcatcgccg acaacgttcc ggttttcaga
     7321 gttcacgctc acaccggact ccaactcatc aacgatttca cccagcatga ccacaggcca
     7381 ggcatgagcc tgagctgatg caacaacata ccgctcccca ctcaaattta agtccccact
     7441 tagcccaatc cgttcccgcg ccaccgcctg cgccatcacc ccatcgccct ctaacttgcc
     7501 cggcgccacc atccagccac gcagcaagcg cgtcgcttcc ggcaaatggc tgtccatcac
     7561 cgcccggcgt tgcgcgccca ggttaaaacc gtcgttttgc actttgacaa acagcacttt
     7621 gtcggtctgc ttgtgcacgc gtttgtccag aatcaggatg ctggttttta cgccgctgta
     7681 ggggttgaac acgccggcgg gcaggctgac caccgccacc agtgacgtat cgaccagcat
     7741 cttgcgcagc gccttgtacg cggtgccgct ctggaagatg atgccctcgg gcaccactat
     7801 tgccgcccgg ccagtcgcgg tcaaatgctc ggccatgtaa tcgacaaaca gcacctcgct
     7861 gcggctggcc ttcacgctga aacgcttgtg cggtttgatg ccgcctttgg ggctcatgaa
     7921 cggcgggttg gccagaatca catcaaacac ctcgttccac ttctcgtcgc tggtcagggt
     7981 gtcgtactcc accacatgcg ggtcgctgaa gccgtgcagg tacaggttga ccaggctcag
     8041 gcgcaccatg tcgggcgaga tgtcgtagcc acggatgttg gcagcgagcc ggattctttc
     8101 gtctggtgta agacttttga gcttgtagcc cccgtcctga ttgcctgagt agctattatt
     8161 ttcagagtga ctggcttgct cggtagcaac atcgtttgcc ggcgtgttgg ccgccaggat
     8221 gtgtttccac gccgagatca agaagcccgc cgtgccacag gccgggtcca aaatggtttc
     8281 gttcttcttc gggtccagca ccgtcaccat gaagtcgatg atgtggcgcg gggtgcgaaa
     8341 ctgcccggca tcaccctggc tgcccagcac gctcagcagg tattcaaacg cgtcaccgag
     8401 cttttcagag tggtcgtagg tgaagacgtt gatttccttc agaaagcttt tgagcgtttc
     8461 ggggtcgcgg tagggcaggt aggcgttttt gaaaatgtcc ctgaacagct cgggcagccc
     8521 ggggttttgc cgcatcgacg ctatcgcctc gctgtacagc gccagcacct caaaaccgcc
     8581 caggccgggt gccagcaggt gtgcccagcc aaatcgggcg tagctgccag caaaaaagct
     8641 gcgtttgcct cccagttctt cggcctcggc atccatgtcg tccatgaact tgtagatcag
     8701 cgcgatggtg atctgctcga cctggctttt ggggtccggc accttgccca ccagaatgtc
     8761 acggcaggca tcaatgcggc gtttggtttc ggagtcaagc atgtgtgggc aaacagtaca
     8821 aaaataatag ctgtccgtgc ttgacgaacg agggctagag gccgatttag tgtgacaact
     8881 cttcccggat gacgcggcgc acggcgattt gccactctgc ctggttgaca tcgcggccgc
     8941 ccaagcgcag tttggcctgg tcaaagtctg cctgcgtgag tttgggcgcg tcgtccctcg
     9001 gagcactaga cgtaattggc gtttttgtaa aagaggtctg tttcatcgct gtctgccttt
     9061 cggcacagaa ttgttttgtg tatagccgtt tttgtcatgt tcgtcgcatt ttcggctaca
     9121 tctgcggtat cacatgaact ggttcagcgg cacgtagtct ttcacgtact cggggatgcg
     9181 ggtgcgccag acccggggca cggccttgaa atcgctcatg ccaaaggctg gatagacgtt
     9241 gaggtcggtc aggcgctgag cctcaataat gtcgcgcagg cggtggtcgg tggcgtaggc
     9301 tttgaagtag tacttcatgg cctgcacggc ggtagcggtg ctgtcggctt ggaagcccgt
     9361 ggcgttttgc tggtcgagca ggaatttgtc gaactcgtct tccagcagtt cgtctttgct
     9421 cttgaagccg gtgatcaggc caaaggtttt ctgcaaaatt tccttgaggc ccaggcgccg
     9481 gtccacaccg gcggcgcggc gcagtttgtc cagggtgaag tactcggcgg gtttgtcaaa
     9541 caactcggtg gtgatgtagt tgatggcctg gtcccactgt tcgtcctgaa tctgggcggc
     9601 aattaccggg tcagctttga ccttgtcctc aaacttctcg aagaacatgc ggtctacctt
     9661 catgccctgg aaacctacct gttgttcgac ctgactggtg atgcggtcgg gtccggtgtg
     9721 ctcaaagggg tggcccggcg ggggtggcgg tggtggtggg cttgggtctt caccacccgt
     9781 gcccggacgc ggcagtttca acacctcgtc gtagttgaat ttttcttcaa agtactcgca
     9841 ggtggcgaaa aagtcgaaca gtttgtaagc ggttttgtgc ggcgcggtga cctgggtttt
     9901 gagcgtttcg tcctgcaact gctcccgaaa gtcatgtttg cgggtgcccc ggcctttgat
     9961 ctgcacaaag tcagacggcg aaaacaccgg gcgcatcagg gccaggttga gcaaatccgg
    10021 gcagtcgtaa ccggtggtca tcatgcccac agtgacgcag atgcgggctt tgctggtttt
    10081 gtactgaggc atggcgttgc ctgtgcccat caggttgttg cttttgtccg tgaaattgat
    10141 ggtgaattgc tgcgcatcgg ggatcagcga ggtgacctgc accgcgaagt ccgactggta
    10201 gcgaccgggg tagatctggt cgctcaattc gttgaggact tgggtgatct tgctggcgtg
    10261 cgcctggctg acggcaaaga cgatggtttt gccaaactca ttgctgatgg ggtcgcacag
    10321 gccgtgcttg agcaaggttt cgcagaacag gcggttggtg ggctccgaga aaaactgctt
    10381 ctcgaagttt ctttgcttga aaacctgttc gctggttagc tcgccgccag cggtatcagc
    10441 gatgacgacg gcggcgtagc cctggtcgga cagcagttgg gtggtgacgc tggtgcgcgc
    10501 atccaccacc agcggattga tcaaaaaacc gtctttgacg ccgtccagca gcgaatagcg
    10561 gtaggtgggc tcgccacttt cgcagccgaa ggtgcggtag gtgtcgagca gcaggcggcg
    10621 ttctgactcg cgcgggtcgc gggtactggg cctggctgca tcaaactttt aaggtagtcc
    10681 ttgggcgtgg cggtcaggcc cagtttgtag ccgacaaagt actcaaacac ggcgcgggcg
    10741 ttgccgccaa tgctgcggtg cgcttcgtca gagatcacca gatcaaagtc ggttggtgaa
    10801 aactgcttgt atttggcgtt gaacagcagg ctttgtacgg tggtgaccac aatttcggcc
    10861 ttgcgccagt cgtcgcggct ttccttgtag atgacgctct tgtagtcgtt tttcagcaag
    10921 gcgatgaagg ccttgttggc ttggtcttcc agctccagcc ggtccaccag aaacagcacg
    10981 cgccgggcat tgccagtgcg cagaaacagc ttgatgacgg cggccgccgt gagggttttg
    11041 ccagtgccag tggccatctc gaacaaaaat cgcgtctggc ccgccttgac tgcggcttgc
    11101 accgtgcggg cggcacgctg ttggtaaagg cgcagaaagc ggaggcggtt tttctggatg
    11161 aaggcatcgc gcgcgatctg atggttccag cccgcttcaa ggtcataagt gggcaactgc
    11221 gtgcgggcga tgtagtccag cggcacatct tccgtggtca gtcgtgccgg gtcgggctgg
    11281 aatttggcgt agcccttgat ggaggcgggc gcggggaacg ccgtgatgac atgcgggttg
    11341 ccgtggctca ggtcccaaaa gtaatgaagg ttaccgttgc tcaggatgac gaagcggcag
    11401 gcttgcgatt tggcgtatct gcgcgcctgc tccttgccga tcagcgggtt tttgtcttcg
    11461 gctttggctt ccagcacgac cagcggaaag ctttgctcgt cgagcagcaa aaagtcgatg
    11521 aagccgtggc tggtggta
//
LOCUS       DAOSLL010000066        11250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5723_length_11250_cov_4.811344, whole genome shotgun sequence.
ACCESSION   DAOSLL010000066 DAOSLL010000000
VERSION     DAOSLL010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11250
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5723_length_11250_cov_4.811344"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..842
                     /locus_tag="PLB25_04415"
     CDS             <1..842
                     /locus_tag="PLB25_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RQC domain-containing protein"
                     /protein_id="HOE40861.1"
                     /translation="WMAYGLQDVVNQRRMIDESPAEEAFKQGLRGKLDALLGLAEATD
                     CRRVRLLHYFGEASGPCGNCDNCLQAPDVWDATDAARKLLSTVYRVKQMSGFGFGAGH
                     IIDILRGKVTDKVAQRGHQGISTFGIGADLSDSQWRGVLRQLLAIDAVAVDAQAFNTL
                     QLTGSSRAVLKGEVPVQLRLSLPKSTERKARHRPEKALARAPAALDATQQARFAALKA
                     WRFEVAKEHNLPAYVIFHDATLAAIAQNSPRSLDDLQGISGIGARKLQAYGDAVLAVM
                     NGL"
     gene            complement(922..2660)
                     /locus_tag="PLB25_04420"
                     /pseudo
     CDS             complement(922..2660)
                     /locus_tag="PLB25_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(2838..4574)
                     /locus_tag="PLB25_04425"
     CDS             complement(2838..4574)
                     /locus_tag="PLB25_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE40862.1"
                     /translation="MKFGSRIATFSAIPAILFVIGLIGSIAALNQSQRAFDRYISSEQ
                     AVERGLTEMYAQGLQMGQAMRNILLDPKNPKAYDNLKAAQADYDKAFTRARESARGSA
                     FEAELEKLPPLRSQQAKAQDKVLALVAASGDAIAVVNAEETPAWRLLKAELLKQGEAA
                     TRSSVNAHQQATRNSNLALGISIGIAILAALVALILNFRIQATLRQELGGDPAEARAA
                     LSTISQGDLTTSMTNSGGPDSLMGVMLQMDAALQTLVAQVRDSAAGIAIASTEIAQGD
                     ADLSARTESQASSLEQTAASMEELSTAVRQNADSARQANQLALTASTVAVQGGEVVAR
                     VVDTMKGINEASRKISDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVR
                     SLAGRSAEAAKEIKSLINASVDRVEQGSKLVDQAGNTMNEVVAAIRQVTDIMGQISTA
                     SDDQSHGVSQVGEAVTMMDQATQQNAALVEQMAAAAGSLKVRSQELVQTVAIFKLGSG
                     GNSGPALLPTAKVRAHPPTASHFNSPDRRTSGVAKGAATRAPKPAAAPPLTAPKSGTA
                     TSASSDSDWETF"
     gene            4755..4895
                     /locus_tag="PLB25_04430"
     CDS             4755..4895
                     /locus_tag="PLB25_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40863.1"
                     /translation="MFSLPVYLDEQVQDYLSAKADAKGVDLSDLVNDLLKREIEMIQA
                     VG"
     gene            complement(5028..6698)
                     /locus_tag="PLB25_04435"
     CDS             complement(5028..6698)
                     /locus_tag="PLB25_04435"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOE40864.1"
                     /translation="MTKPLQQIGAASSTAAPPVFKRKIALAPVLHALAASILIATGSL
                     HTPGLSAQTGADSSALAAGSLPLLGDGSERTSGAERRLGDRIVRELYRDPDYLDDPVL
                     SDYVQGIWQPLLAAARLRGDLTAELDERFAWQTLMGYDRSVNAFALPGGYFGLHLGLL
                     AVVSSRDELASVLGHELSHVTQRHVSRLISKSNQQTPWMIGAMILGALAASKSADAGS
                     ALMVGGQALTAQNQLNFSRDMEREADRLGFGVMTQAGFEPIGFVTMFDKLQQAARLND
                     NGAYPYLRSHPLTTERIADMQSRLPLGMPQGVVPTPTVLHAMMVARARVLSNAGIDAL
                     RGWQGQADDPGLLTQPRAKQVAALYGATMAASRLRDAALAQRNQQRLQTLVAGDRPAL
                     QQARWLGIEIALAANDLAVAQALLTQANGAAAQATDSADRSPQRPALLLQAQVALKAG
                     TDSALESAAQGLQTWVSQQTQDALAWQSLASIYAAQGQGLRALRAQAEARVAQLDYPG
                     ALDRLRAAQDYAAVASATSSSARHIDASIIDTRRHQIELLLKEQAPDR"
     gene            6741..7241
                     /gene="moaC"
                     /locus_tag="PLB25_04440"
     CDS             6741..7241
                     /gene="moaC"
                     /locus_tag="PLB25_04440"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HOE40865.1"
                     /translation="MTATNSFTHFDALGQAHMVDVAAKVATHRIGVASGRIEMLPATL
                     ALIVSGSSKKGDVLGIARIAGIMAAKKTSDLIPLCHPLALTRVAIDFEAAQADASRAG
                     AIICTATVETVGPTGVEMEALTAVNVALLTIYDMCKAVDRAMVMTGIKLLEKHGGKSG
                     SFVAGG"
     gene            complement(7605..7868)
                     /locus_tag="PLB25_04445"
     CDS             complement(7605..7868)
                     /locus_tag="PLB25_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40866.1"
                     /translation="MTSTEFTSLIVASLSVVGMAFTAVLSGWFQSRTAKAQIHKDLLL
                     VELQAQIREEQLVREKFEGLTTQLNDLSFGLMCHSRFLADQEI"
     gene            complement(8546..9247)
                     /locus_tag="PLB25_04450"
     CDS             complement(8546..9247)
                     /locus_tag="PLB25_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HOE40867.1"
                     /translation="MTDSVWRTSWFDSGVVQRTMLAWRGVEAQHVVSTMRLVDDTDEQ
                     DVLEQLLEASKPSMPTMKLPKHYLLATPFRYRPQHSGRFRRAGTLGLWYGAEALFASC
                     AEVAYWRYRFIMDSVPLLNTDLLTEHTFYQAKVEGRAIELMSAPWVAASAAWTHGSDY
                     TETQAVAEAARDRGVQWICYESVRAPPARCAAVLDVEALEMVDQGKTQQTWHCKATRT
                     SVMMVHGADRHVWNF"
     gene            complement(9244..9618)
                     /locus_tag="PLB25_04455"
     CDS             complement(9244..9618)
                     /locus_tag="PLB25_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HOE40868.1"
                     /translation="MNTKTAPKPDPGYVLAKATVRATVLLGLSGAALARIIGVSEPTV
                     SRIRKGEYPLDANSKVGELALLLVRVYRSLDALVGTDDQKRKDWMRGMNKALGGVPLQ
                     LIERPDGLVATLNYLDGMRAPT"
     gene            9685..9993
                     /locus_tag="PLB25_04460"
     CDS             9685..9993
                     /locus_tag="PLB25_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40869.1"
                     /translation="MIITFVVAACLLRSQQSWCPLSWRSLRKCSQIVCTIRVVRAEHD
                     NYFYPDFVVCPRHYPADEPIARLLETKDDTKDAARKSQHSPAGYGKVLFLTPDGNRMR
                     "
     gene            complement(10136..10711)
                     /locus_tag="PLB25_04465"
     CDS             complement(10136..10711)
                     /locus_tag="PLB25_04465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOE40870.1"
                     /translation="MPPDHFTAVPLDISYRLLNHGPTVLVSAAHGGISNVMAAAWACV
                     LDYGQSPKVTVVLDKSTYTRELLMASGEFALQLPPKALASLTVGVGSDSAREVPDKLI
                     KHAVQLFKAPGNTVPLVAGCVGWLICKWVPEPHNEQTYDLFIGEVVAAWADERVFCDG
                     HWQFESAPDELRTLHHIAGGRFYAIGEPAQG"
     gene            10944..>11250
                     /gene="umuD"
                     /locus_tag="PLB25_04470"
     CDS             10944..>11250
                     /gene="umuD"
                     /locus_tag="PLB25_04470"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011458711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOE40871.1"
                     /translation="MPISDVPLLVLLLKNRVNAGFPSPAEDLGAKRIDLTQLLITHPQ
                     ATYFLRASGHSMEEAGISDKDILVVDRAIKPRHNHVVVAVVDGDFTVKRLYQRAGRVK
                     "
BASE COUNT         2184 a         3313 c         3336 g         2417 t
ORIGIN      
        1 cctggatggc ctacggcctg caggacgtgg tgaaccagcg ccgcatgatc gacgagagcc
       61 cggccgaaga agctttcaag cagggtttgc gtggcaagct tgatgccctg ctgggcctgg
      121 ccgaggccac cgattgccgc cgggtgcgct tgctgcatta ctttggcgag gccagcgggc
      181 cctgcggcaa ttgcgacaac tgcctgcaag cgccggacgt gtgggatgcc accgatgccg
      241 cgcgcaagct gttgtccacc gtctatcggg tcaaacaaat gagcggtttt ggctttggtg
      301 ccgggcacat catcgacatt ttgcgcggca aagtcaccga caaggtggcg cagcgcggtc
      361 atcaaggcat cagcaccttt ggcattggtg ctgatctgag cgactcccag tggcgcggtg
      421 tattgcgcca gttgctggcc atcgacgcgg tggcagtcga tgcgcaggcc ttcaacacgc
      481 tgcagctcac cgggtcatcg cgtgctgttc tcaagggcga ggtgccagtg caactgcgcc
      541 tgtccttgcc caaatcaacc gagcgcaagg cacgtcatcg gcccgaaaaa gcccttgcca
      601 gagcacccgc tgcgctggat gcgacacaac aggcgcgctt tgccgccttg aaagcctggc
      661 gctttgaggt ggccaaagag cacaacctgc cagcctatgt gatttttcac gatgccacgc
      721 tggccgccat tgcgcagaat tcgccacgct cgctcgacga tctgcagggc atcagcggta
      781 tcggggcgcg caagctacag gcgtatggcg atgcggtgct ggcggtgatg aacggtttgt
      841 gacacaaaaa aacagttaaa tcggcctcca gcgcccgtcc ttattgcgct ggcagctatg
      901 ttttatgtag ttaaaccggg ttcagaaggt ttcccaatcc gcgtcgctgg tcgctgtgct
      961 tgacttgggc gcagccagcg gcggcggtgc agcgggtttg ggggcgtgcg ctgcagcgcc
     1021 tttggcgaca ccgcttgtcc gtcgatcagg gctgttgaag tggctgacca tgggtggctg
     1081 ggcgtgcaca gtggcgctgc cagacagtgt tgtcggcttg tgttcgccct cgccaagttt
     1141 gaacacagcc acggtctgca ccagattgcg tgcctgcacc ttcaggttac ccgttgccgc
     1201 tgccatctgt tcaaccagcg aggcgttttg ctgcgttacc tggtccatca gtgtcacggc
     1261 ttcgccaact tgcgacacgc ccaagctttg ctcattactg gcattgctga tctggcccat
     1321 gatgtcggtg accttgcgaa tcgacgacac cacctcgttc atggtggtgc cggcccgatc
     1381 aaccagcagg tttccctgct cgacccgctc cacgctggcg ttgatcaagg tcttgatttc
     1441 cttggcggcc tctgcgctac ggccagccaa gctgcgcact tcggatgcca ccacggcgaa
     1501 acctcgcccc tgttcgccag cgcgcgctgc ctctaccgcc gcattgagcg ccaggatatt
     1561 ggtctgaaag gcgatgccgt cgatcacgct gatgatgtcg gaaatcttgc gggatgcctc
     1621 attgataccc ttcatggtgt cgaccacctg cgccacgacg gcaccaccct gcaccgccac
     1681 ggtactggcg ttctgggcga gctggttggc ttcgcgcgcc gagtcggcgt tttggcgcac
     1741 tgtggcgcta agctgttcca ttgaggcgga agtttcttcc agggagctgg cctggctctc
     1801 ggtgcgtgcc gagaggtcgt tgttgccctg cgaaatttct gcgctggcgg ttgccaccga
     1861 ttccgcgccc tggcgcacct gcgtaaccac cgaaatcaac tgactttgca tgtttgacag
     1921 cgatgccatc agttgaccaa tttcatcgtg actgccagat gtcaagggct ggctcaggtc
     1981 accttccccg atgcgctggg ctgccaggtt gaccttgctg atgccccgcg tgaggatacc
     2041 ggtcagactc ttgctggaca atacggcaac aaccagacag gcaaacagca gcacaacgag
     2101 cgtgatgcgg atttgcctca gcaactcgtc aacgtgcgcg ccgcccgcac gttgacgagt
     2161 tgcccatcga caagttcctg cgttacccgg atgtagtcga cggtacctgg taccagcttg
     2221 ctgttcacca gggcttttgc gccagcgccg tcgccagcag ctttcaggct gttggcctgg
     2281 tcacgaatgg ttacccatgt ggatctgacc tcgcccacgg ctgcggcgcg ttttctggtg
     2341 gcatcgtcct gcgccagcgc caaaacggcc ttttgggttt cgctggtgct gcgtgtcaac
     2401 tcttctatcg ggcccttgaa aaactccaac atgtctgttc cgtcggagta cgccaccgcc
     2461 agggagcgtg ccgagttctg ttgcacgttg gctcgccagg tggcggccaa attcagcaaa
     2521 tgtgtctcgg agtccatgcg ggtggcggcc cgcgatacca actcaatttg ccagagcgtg
     2581 atggcagcca atacaaaaaa agtcagtgtg atcgcgaaaa acccaaggct aagctttgta
     2641 gaaatcttca tggaatccac cagaacgtct ccttcaaagt taataaaaca attccatcaa
     2701 aatgggcttt agcgatgcct taccaaaggt ttgcaagcaa aacacgccgt cgcgaaacac
     2761 gctgagcctg cgggatagct ggcctggtcc attggccggg atggctcagg gttggcctga
     2821 cggtggctgc ggatggtcta aaaagtttcc cagtccgaat cgctgctggc gctggtcgcc
     2881 gtgcctgact tgggcgcagt cagcgggggc gctgcagcgg gtttgggggc gcgtgttgcc
     2941 gcgcctttgg cgacaccgct tgtccgtcga tcagggctgt tgaagtggct cgccgtgggc
     3001 ggatgggcgc gcactttcgc cgttggcaac aacgccgggc cactgttgcc gccgctgcca
     3061 agtttgaaga tggccaccgt ttgcaccagt tcctgcgagc ggactttgag actgcccgcc
     3121 gcagccgcca tttgttcgac cagggcggcg ttttgctggg tggcctggtc catcatcgta
     3181 acggcctcgc caacctggct gaccccatgg ctttgatcgt cgctggcggt gctgatctgt
     3241 cccatgatgt cggtgacctg ccgaattgcg gcaaccacct cgttcatggt gttgccggcc
     3301 tgatcgacca gcttgctgcc ttgttcgacc cgatcgacgc tggcgttgat cagcgacttg
     3361 atttccttgg ccgcctcggc gctgcgcccg gccaggctgc gcacttcgct ggccaccacg
     3421 gcaaacccgc gaccctgttc accggcgcgg gccgcttcca cggccgcatt aagtgccaga
     3481 atattggtct gaaacgcaat accgtcaatc acgctgatga tgtccgatat tttgcgcgag
     3541 gcctcattga tgcctttcat ggtgtccacc acccgtgcca ccacctcacc gccctgcacc
     3601 gcaacggtac tggcagtcaa agcaagctga ttggcctggc gggccgagtc ggcgttttga
     3661 cgcacggcgg tgctgagctc ctccatgctg gcagcggttt gctccaggga actggcctgg
     3721 ctttcagtac gtgctgacag gtcggcatct ccttgggcaa tctcggtgct ggcaatggca
     3781 atgccggcgg ccgagtcgcg cacctgagct accagggttt gcaaagccgc atccatttgc
     3841 aacatgactc ccatcaggct gtccggccca ccgctgttcg tcatgcttgt ggtcaagtcg
     3901 ccctgtgaaa tggtcgacaa ggcagcacga gcttcagccg ggtcaccacc gagttcctgg
     3961 cgcagcgtgg cctgaattct gaagttcaaa atcagggcca ccaacgcggc aagaatggca
     4021 atcccgatag agatacccag ggccaggtta ctgttgcgtg tggcttgctg gtgggcgttg
     4081 actgaactcc tggtggcggc ctcgccttgt ttcaataact cggccttgag caggcgccag
     4141 gctggcgttt cctcggcatt gacgactgca atggcatcgc cgctggcggc caccagggcc
     4201 agcactttat cttgcgcttt ggcttgctgg ctgcgcagcg gcggcaactt ctccagttcg
     4261 gcctcgaacg cgctgcctct ggcgctttcc cgggcgcgcg tgaatgcctt gtcgtagtct
     4321 gcctgggcgg ccttcagatt gtcatatgcc ttggggtttt tggggtcgag caaaatgttg
     4381 cgcatggctt gtcccatttg cagaccctgc gcatacattt cggtcaggcc gcgctccact
     4441 gcctgttcgg aactgatgta acggtcaaac gcacgctggc tttggtttaa agcggcgatg
     4501 ctgccgatca ggccaatgac gaacaaaatg gctgggatag ccgaaaacgt ggcgatcctg
     4561 gatccgaact tcatggcgtt actccaaagc gaatggcaaa attttacgct tgaggggtca
     4621 attggaccca agtgtcaaga cattttttca gttaatttag gctgccctgt tttccaggat
     4681 aaatttgttc ttatggcatc ttcgtcattc ccgcctgcgc ggaagaagat gccaaaggaa
     4741 caaatttacc ctgggtgttt agcctgccgg tgtatctgga tgagcaggtg caggactatt
     4801 tgtcggccaa agctgatgcc aagggggttg acctgtcgga cttggtcaac gatttgctca
     4861 agcgcgagat tgagatgatt caggcggtgg gctaaggcac gctaccctag cgccctgcgg
     4921 ctgtgtgatc ttgagcatgt gtttttgtgg cacgcgcttg cccggccaat gcggcggctc
     4981 aagacccacc tttgccaacc tgcccggtgt tggttatcga tgctggttca gcggtcgggc
     5041 gcttgctctt tgagcagcag ctcgatctgg tgccgccgcg tgtcgatgat ggacgcgtca
     5101 atgtgtctgg cgctgctgct tgttgcgcta gccacagcgg catagtcctg cgccgcccgc
     5161 aggcggtcca gcgcgccggg gtagtcaagc tgcgccaccc gcgcttcggc ttgtgcgcgc
     5221 aaggcccgca ggccctgacc ctgggcggca tagatgctgg ccagcgattg ccaggcaagc
     5281 gcatcttgcg tttgctggct gacccaggtt tgcaagcctt gtgcggctga ctcaagcgcg
     5341 ctgtcggttc cggccttcag cgcgacctgg gcttgcagca acagcgctgg ccgctggggc
     5401 gaccgatcag ccgaatcagt ggcctgggcg gctgcaccat tggcctgcgt cagcaaagcc
     5461 tgagccacgg ccagatcatt ggcggccagg gcgatttcga tgcccagcca gcgtgcctgt
     5521 tgcaaggccg ggcggtcacc tgccaccagg gtttgcaggc gttgctggtt gcgctgtgcc
     5581 agcgccgcgt cacgcagtcg gctggcagcc atggtggcac cgtacagcgc cgccacctgc
     5641 ttggcccgtg gctgggtcaa aaggcccggg tcgtcggcct gaccctgcca gccgcgcagc
     5701 gcgtcaatgc cggcgttcga caacaccctg gcgcgcgcca ccatcatggc gtgcaacacg
     5761 gtgggagtcg gcacaacacc ctgcggcatg cccaatggca gacgcgattg catgtcggca
     5821 atgcgctcgg tggtcagcgg atggctgcgc aaataggggt aagcgccgtt gtcattgaga
     5881 cgggcggcct gttgcagctt gtcaaacatg gtgacaaagc caatcggctc aaaaccggct
     5941 tgcgtcatca cgccaaaacc aagccggtca gcctcgcgtt ccatgtcacg cgagaaattc
     6001 agttggtttt gcgcggtcag ggcctgtccg ccgaccatca gggcgctgcc agcatcggca
     6061 cttttgctgg ccgccagcgc ccccagaatc atggcaccaa tcatccacgg ggtttgctgg
     6121 ttgctttttg agatcagcct ggagacatgg cgctgcgtga cgtggctgag ttcatgcccc
     6181 agcactgaag ccagttcgtc gcggctgctg accaccgcca gcagtcccag atggagcccg
     6241 aagtagccgc ccggcagggc aaaggcattg acgctgcggt cgtaacccat cagggtctgc
     6301 caggcaaaac gttcgtcgag ttcagcagtc aggtctccgc gcaggcgcgc tgccgccagc
     6361 aacggctgcc agatgccttg cacatagtca cttagcaccg ggtcgtccag atagtcgggg
     6421 tcgcggtaca gctcgcggac aatgcggtcg cccaggcgac gctcggcacc gctggtcctc
     6481 tcgctgccgt cacccagcaa aggcagcgaa cccgccgcaa gcgcgctgct gtcggcaccg
     6541 gtctgggccg acaggccggg cgtatgaagg ctgccagtag ctattaaaat agaagctgcc
     6601 agcgcatgca gcacgggggc tagagctatt tttcgcttga aaactggcgg agctgcggtg
     6661 ctgctggcag cgccaatctg ctggagtggt ttggtcatgc ccgtatgatg ccagccagat
     6721 gtaaaggttc ggtgaatccc atgacagcaa caaactcttt cacccatttt gatgccctgg
     6781 gccaggccca catggtcgat gtagccgcca aggtggccac ccaccgcatt ggcgttgcca
     6841 gcgggcgcat tgaaatgttg cccgccacgc tggcgctgat cgtctcgggc agcagcaaaa
     6901 agggcgacgt gctgggcatt gcccgcattg ccggcatcat ggccgccaag aaaaccagcg
     6961 acctgatccc gctgtgccat ccgctggcac tgacccgtgt tgctattgac tttgaagctg
     7021 ctcaggcaga cgcatcaagg gctggcgcca ttatttgcac tgcaaccgtg gagaccgtcg
     7081 gccccaccgg ggtcgaaatg gaggccttga ccgccgtcaa cgtggccttg ctcaccatct
     7141 acgacatgtg caaggcggtg gatcgggcca tggtgatgac tggcatcaag ttgctggaaa
     7201 agcacggcgg caagtcgggc agtttcgtgg caggcggcta agtggttgat ttgcaaaggt
     7261 gttttgagcc gaagggattt ccgggattga ggtcgttagg ttgaatcgat taccccttct
     7321 tggctgtttt ctgaatgagt tttgcttttt gtccctcgtt tggcccactc aaaggaaaac
     7381 gatggcagct acacgggtaa gaagaggaaa acttcgtcga gcgtgccaac ttggttgttc
     7441 cgcccactgc ggacccgcat ggtgggtggc gtggggggcg accgtcagaa accgttgcct
     7501 acccgattag gtcgcaaacg aggatacaga cccacgtttg tgccgccgct cggcaacttc
     7561 cgacaaaaac gccttggtca gccggtcagt cgcctctccc aagttcagat ttcctgatcc
     7621 gcaaggaacc gtgaatggca catcagacca aacgaaagat catttagctg tgtcgtcaac
     7681 ccctcaaatt tctcgcgcac aagttgctct tcacgtattt gtgcttgcaa ctcgacaagc
     7741 agtaaatcct tgtgtatttg cgcttttgca gtacgactct gaaaccaacc agacagtact
     7801 gcggtaaatg ccatcccaac gacgctcagc gacgcaacta tcaaggatgt gaattctgtg
     7861 gaagtcacga tatcctccaa atgtggccga acgaatagcg tttatcttgc gcgctgcaat
     7921 gctgatgaag acacataacg ccaaacgcgc aagataaacc ttgctggact gaaccgccgc
     7981 cgacagacgg ctatcgatat ggggatggaa agaaatcctg gcgttttgca ggcgggtcat
     8041 cgcgcgaact tttcctcaac acgcccccca gacattgatc ccatgttgaa aacggccacg
     8101 gtcggtcgtt accaggctgg tgggacaacg agcaaaacga atgcatacct gccgatggtt
     8161 gatgcgagcc caacaccgcc ccaagtcggg tgaagtgtcc aaaagagcca gtcctgacgc
     8221 acaagcttgc gtttcacggc ggtcctcgct cgttggttgc caaacataac caggggcagg
     8281 aaaatgccca gagctcgcca tgacgcaggc gaccctcaag tgccccacct tgcacgccag
     8341 gcaagaggca gaagagtgat cgacgacaac ttgaccggct tggaaaatca gtttttcgcg
     8401 ctcactgcga ggccccagaa gtctcctagc tgaagctggt cggctggaag ccggtagatt
     8461 taaccttgtg gtgggtatta aattcaggaa agatgcgctg aactgcggcg acaaccttca
     8521 ccttggccgc gccggatgag gccagctaga aattccaaac gtggcgatca gcgccatgga
     8581 ccatcatgac ggatgtcctc gtcgccttgc agtgccaggt ttgctgcgtc ttgccttggt
     8641 caaccatctc aagtgcttca acgtccagga ccgcagcaca gcgagctggc ggggctcgca
     8701 cggattcgta gcaaatccac tgcacgcccc tgtcccgtgc agcctctgcc accgcctggg
     8761 tttcggtgta gtcactgcca tgcgtccatg ccgctgatgc tgcaacccac ggggcgctca
     8821 ttaactcaat ggcacgaccc tcgacctttg cttgatagaa agtatgttca gttagcaggt
     8881 ccgtgttcaa caatggcacg ctgtccatga tgaagcgata gcgccaatag gcgacctccg
     8941 cacaggaggc gaagagtgct tcggcaccgt accaaaggcc gagggttcct gcacgccgaa
     9001 aacggccgga gtgttgaggt cgataccgaa atggcgttgc aagcaggtag tgtttcggca
     9061 atttcattgt cggcatggat ggcttgctgg cctccagaag ttgttcgagc acgtcttgct
     9121 catcagtgtc atcgaccaag cgcatcgttg agaccacatg ctgcgcttca acgccccgcc
     9181 aagctaacat tgtcctttgt acaacaccgc tgtcaaacca tgaagtgcgc catacggagt
     9241 cggtcatgtc ggtgcccgca ttccatcaag gtagttcagg gttgcgacca ggccatcagg
     9301 ccgctcaatg agttgcaggg gcacgccgcc aagcgcctta ttcattcccc tcatccaatc
     9361 cttgcgtttt tgatcgtcgg ttccaaccaa cgcatccaaa gagcggtaaa cccgaaccag
     9421 caataaagcc aattcgccga ctttggaatt ggcgtcaagg gggtactcgc ccttgcggat
     9481 gcgcgagacg gttggttcac tcacgccgat gatccgagcc aacgcggccc ccgacagtcc
     9541 caataaaacg gttgcgcgaa ctgttgcttt ggccagtacg tagcctgggt caggcttggg
     9601 cgcagtcttg gtgttcattt gctctccatt gctttctttg gaaacatttt tacacgatca
     9661 ttgaataatt gcatgtattt ttagatgatc atcacatttg ttgtggcggc ttgtttgctt
     9721 cgtagccaac agtcatggtg tccgttaagt tggcgttcgt tgagaaagtg ttcgcagatc
     9781 gtttgtacga ttcgggtggt gcgcgcagaa catgacaact atttctatcc cgacttcgtg
     9841 gtttgtccgc gccactaccc ggccgatgaa ccaattgccc ggttgctgga aaccaaagac
     9901 gacactaagg acgctgcgcg caaatcccag cattcacccg ctggctatgg caaggtcttg
     9961 ttcctgaccc cggatggcaa tcgcatgcgc tgagtcaacg atgatggcag cctgggtgct
    10021 gtactggatt ttgatgacat gcagagtgtg ctgggccggc tggcggcgac acgaccggtc
    10081 gcggttgacg ttcgcgttcc ttgatggctt ggtaatggct gcaagcggcg ttcacctagc
    10141 cctgtgctgg ctcaccaatg gcataaaacc ggccaccggc gatatggtga agggtgcgta
    10201 actcatccgg tgcagattca aactgccagt gaccgtcaca aaagacacgc tcatcagccc
    10261 aggcagcaac cacttcacca atgaacaggt cataggtctg ttcgttgtgt ggctcaggga
    10321 cccatttgca tatcagccaa ccgacacagc ctgccaccag cggaacggtg ttgcccggcg
    10381 ccttgaacaa ctggacagcg tgcttgatca gcttgtcggg cacctcgcgg gcgctgtcgc
    10441 tgcccacccc cacggtcaac gaagccagtg ccttgggggg cagttggagc gcgaactcac
    10501 cgctggccat cagcagttca cgggtgtagg tggacttgtc cagcaccacg gtgactttcg
    10561 gcgactgacc gtagtcgagc acacaggccc aggcagcggc catcacgttg gagataccgc
    10621 catgggcggc agaaaccagc acggttggcc catggttgag caggcggtag gatatgtcga
    10681 gcggtacggc agtgaagtga tcaggaggca tggctggctg ggttcaaaga gtcagtgttg
    10741 cggacatcgt agcgcagcgc ttgtccccaa taagcgggac caaccctgtg gacaagacac
    10801 gggacaagtc tggcagccca ttgcggctgt ggcttggcga tgagtgctca aactttcggc
    10861 accgggcttg ttgggagttg tcaggcagtc cggcgacaac atatactgta caaatgatca
    10921 gcattgtttc aagtcttctg cctatgccca tttccgacgt accgctgctg gtgctgctgc
    10981 tcaagaaccg tgtcaatgcc gggttcccat cacccgccga agacctagga gccaaacgga
    11041 ttgacctgac ccaattgctc atcacccatc cccaagccac ttacttcctg cgagctagtg
    11101 ggcactccat ggaggaggcc ggtatctccg acaaggacat tctggtggtg gaccgagcca
    11161 tcaagccccg gcacaaccac gtcgtggtgg cagtcgtgga cggtgacttc accgtcaagc
    11221 ggctgtatca gcgtgccggg cgcgtcaagc
//
LOCUS       DAOSLL010000067        11231 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5732_length_11231_cov_5.302255, whole genome shotgun sequence.
ACCESSION   DAOSLL010000067 DAOSLL010000000
VERSION     DAOSLL010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11231
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5732_length_11231_cov_5.302255"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2124)
                     /locus_tag="PLB25_04475"
     CDS             complement(<1..2124)
                     /locus_tag="PLB25_04475"
                     /inference="COORDINATES: protein motif:HMM:NF018873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HOE40872.1"
                     /translation="MRFISKTAALVMIFLTFLLFVPLAQAFKPASELWGHTMITKGVL
                     GEKMGYSFGDRLTVDRFSFYWVNGPDSFVSDDALEWIIYGVQSRDLFGHLLIDGSIYR
                     GPWGPLVDCSNDKNIPVYKDARPTVTSGDLVTWHQGTLCFNVTSDLSDIDGHFDNDNI
                     QGSTDAIRAHAIYAINLASEASTTTDYTVATTKKAAARMMLGKAIHTLQDFYAHSNWA
                     DTHLSSNELFTPITDYLVSGDVKVFDGSKYHGAQPIPPKGSTSFPARSRYTDLTDTCK
                     ASSGSWLLRNSWAVNDGNSELVGDYHKESFDNDPNFITTGAWWGDVFYTPASSTFGGN
                     GVGAGSDTPGKVRCDHGSAISVGLNVAHVQGIAKDSPWMPLGSEGPVDQSGLHTNQHD
                     DSAMEKVYKESGESKDVIGNWSTDLHQRASYLAALHTKLFMTKFVELVKQKFPYPTDA
                     NGVLKTLFGGSTEFVVVKDSVNIFVVDRSATMADILPTLVTQIISSMVDGTHYVYGKT
                     YGLVDFADSDDVSELFLGTKAEIAERLNQLVARGGGGCAAPVFKALNNALNGADKSEL
                     WASNVFYFTDASVSDAAAFEATNTLAAKAQRAKITPVVSGTCGSTIDPAHTRVATGSN
                     VLVIDHSAGDVASALAVLLPAPWTLGASTTSLAKSALARNSAKALADAVATENNGRLI
                     YVEEGAVNVARSIDIPVEAGATALAF"
     gene            complement(2141..4165)
                     /locus_tag="PLB25_04480"
     CDS             complement(2141..4165)
                     /locus_tag="PLB25_04480"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOE40873.1"
                     /translation="MNYLSMLRAVALCLSLGTSVAQAVTCAASIAPSNPDSVYTPNLD
                     PTVAVNQDNTVTHIPTGLVWKRCSEGQSWNGATCTGTATGHTWAEALALAGNSRFAGQ
                     SDWRLPNIRELRRLVEECRTSPSINDAFFPATPSGYFWSGSPGAVNSLWAWYVSFQEG
                     NANSGIRGNGGYHVRLVRGGQSFDSLAPATPTVTSVSPTSAIVGINTTFTVTGTALTT
                     GMGFTVQDCAPNNAEVGIGSATQRTFACVPEVAGSKHVLVKDTPGGTVLFQSASPATP
                     GAQPINVTVTTSAPVLSAFAAAQFNDGIGGAFTVSDVDGGSLAVARVLVAADAAAHPF
                     PCFVDSSTSLGSRAFTIGTGNGVDAVAGGCAALFANGTRTLYVKVEAKDPQGNQASGT
                     LISVPVTYPGNQWSLTVTGNGTGSGTVISAPAGIDCGSTCSASFNTGSVVTLTAAPAS
                     GSTFTGWTAGACTNADADSTRICQVTMDAAKSVAATFSLAGAAACGSANGQAYPEYYL
                     PTDATEFCSIGSASALRQTGSSFAWTCSVAGGATVSCATVAANACTLSQVWKIQRANL
                     SGAMSTSIASNQANITQTYNVNCTSGRFGVVRMPGIAKLAVENSYALVQSSCFTSLTR
                     SPLIPRQGGPTTEAEVRDAIAAAGDGTTDPTVDLGNHRVVGPLATAICQY"
     gene            complement(4288..5091)
                     /locus_tag="PLB25_04485"
     CDS             complement(4288..5091)
                     /locus_tag="PLB25_04485"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOE40874.1"
                     /translation="MSNPSPRSGAARLLLASLSPLLLCTSLAQAAAGALNDTGITFCG
                     DASTNTANCATVADDGGAFPRQDARYGRDAQAAAGQLSKIGGGGAGFDFTKIANNGSV
                     LPATAGPGSGPGEWACTKDNVTGLIWEIKTSSGLRSLSHTYTWYNRNPAANGGGVGTA
                     SGGTCFASGSCDTEKYTADVNAIGLCGASDWRMPTVKELESIVNYGRTNPSVEWLYFS
                     DTQSAGFWSGSPDAYSSNNAWYVNFDNGYAYSDGPRANAGLVRLVRSDE"
     gene            complement(5482..5745)
                     /locus_tag="PLB25_04490"
     CDS             complement(5482..5745)
                     /locus_tag="PLB25_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40875.1"
                     /translation="MTPAQRVKRNRDKAREAVLMSLEKMTSSNTAALLGGLARQIKLI
                     ETEPQFADSAREGAAMLMGELCKRYEINLPNSNPAKFTQPPTG"
     gene            complement(6095..6439)
                     /locus_tag="PLB25_04495"
     CDS             complement(6095..6439)
                     /locus_tag="PLB25_04495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HOE40876.1"
                     /translation="MKTKIIKKEIDLQNLPPLTDEQRVRLSALANKPDSEIDYSDIPP
                     LGDDFWKNAVRNPFYKPVKQVTTVRVDSDVLLWLKSRGKGYQTRINAILRDAMLNSIQ
                     HHKGETKAKSTS"
     gene            complement(6420..6716)
                     /locus_tag="PLB25_04500"
     CDS             complement(6420..6716)
                     /locus_tag="PLB25_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006682805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HOE40877.1"
                     /translation="MPIRFEWDEAKALRNLRKHGVAFDDAVGVFADPFAVMKQDRIEG
                     GELRWQTIGMVEGHLMLLVAHTFSETQDDIEVIRIISARTADRKERKRYENKDY"
     gene            7606..8883
                     /locus_tag="PLB25_04505"
     CDS             7606..8883
                     /locus_tag="PLB25_04505"
                     /inference="COORDINATES: protein motif:HMM:NF013235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HOE40878.1"
                     /translation="MLTVVEPDKEFRKANNAVSVRVKSGPTLSMMGRRLFNVMLYHAQ
                     HLGAPGVNAPEPWGACPNPGDYYWIPLPDIAHDAAWGSKDHKLVVWTLQQLQTTLVES
                     DDPSGRFSSVQLIGSVHIVKGSGRRPSLVGWEFPRSTRDILSNPDFYTKLSIFHLTSL
                     QTTGGAALYEIAKRYLTNIGGKTSKNHWHWWHDTLTGRQIGSIPYPEYRYFKRDVLTP
                     AIEEVNRTDIHVVPTESRQGRLVTDLQFMVTLVTQTSLELPPGPVIDSALLERVCALG
                     FTERQACELLGTMADDFLRATLELVEERLNDKSLEPLKSPAAFFRKALKDDYVSTAKI
                     KPKVLPDKPIPAKVVEVESPAATERKGRVTQALLEFDALAAMEQNNVLGEFYLTSATH
                     KRLKPGGTMFRKTLGNWLVDATDRPLAQLLPSN"
     gene            9004..9633
                     /locus_tag="PLB25_04510"
     CDS             9004..9633
                     /locus_tag="PLB25_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HOE40879.1"
                     /translation="MIILIGGEKGGSGKSTTATNLAVYLMLEGHDVMLLDCDKQGTAT
                     KWATRRNEAGYKEVHSAQKLGDVYKTAIDLSQRYGHVIIDAGGRDSRELRTGMVAADL
                     IYIPLKASQADLETLPVVDELITLSRAMNPNVKARTLLCMAPTNDRIKEVEDARALLA
                     NFPDLPLSECIIRERKIYRDALLLGRGVVEMKNSEARAEIQLFGQEVLQ"
     gene            9630..9890
                     /locus_tag="PLB25_04515"
     CDS             9630..9890
                     /locus_tag="PLB25_04515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40880.1"
                     /translation="MSGFKVVPKKPAVDPDVLAKFASGADKIQGAMPWEGLDDKRRMA
                     AFSLRLTACELAKLKHIASTTPDSMHEFCIRAVKKALDEKIV"
     gene            complement(9946..10521)
                     /locus_tag="PLB25_04520"
     CDS             complement(9946..10521)
                     /locus_tag="PLB25_04520"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOE40881.1"
                     /translation="MKQGKAPYIGLAELAATLAAQTGIHATRNQSILMLSHYMGLRAM
                     ELAKLTIGDLYDPQTREVRESVRLLSCMTKGQKFREVYLTHVPSRQILATYLASRGLR
                     YMDAPLFLSQRGGAFSPNTMQRLLAIMYQRAGVVGSSHSGRRSFATHLIETGADVFAI
                     KELLGHTSIATTQRYFSSNPSRMKKFVSLLI"
     repeat_region   10907..11174
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=10907..10934
                     /rpt_unit_seq="cttctcaaccacctgcacggtgggtcac"
BASE COUNT         2656 a         2959 c         3000 g         2616 t
ORIGIN      
        1 aaatgccaac gcagtggcac ccgcctcaac gggaatgtca atgcttcgtg cgacattgac
       61 tgcgccttct tcaacataaa tcaggcggcc gttattttcc gttgccactg catcagcaag
      121 cgctttagcg ctattgcgtg ccagtgctga cttggccaag gatgtcgtgc tggcccctaa
      181 agtccaaggc gctggcaata agactgctag tgcagatgcg acatcccctg cgctgtgatc
      241 aatcaccaat acattgctac cagttgctac ccgcgtgtga gcggggtcaa tcgttgaccc
      301 gcaggtgcca gacaccaccg gcgtgatctt ggccctttgt gcctttgcag ccagggtatt
      361 ggtggcctca aaggcggctg catccgacac cgaggcatct gtaaagtaga aaacattact
      421 tgcccaaagt tcgctcttgt cggcgccatt caatgcattg ttcagggctt tgaacacggg
      481 tgcggcacag ccaccaccac ctcgggcaac caattgattt aaccgttccg ctatctcagc
      541 cttggtaccc aggaacaact cggaaacgtc gtcactgtca gcaaagtcca ccagaccata
      601 tgtcttgccg tatacatagt gcgtgccatc caccatggac gaaataatct gggtaaccaa
      661 agtcggcaag atgtctgcca tggtggcaga gcggtctaca acaaaaatgt tgaccgagtc
      721 tttgaccaca acaaattcag tacttccacc aaataacgtt ttaagaactc cattggcatc
      781 agtgggatat ggaaactttt gtttaacaag ttcaacaaac ttggtcataa aaagcttggt
      841 gtgcagggcc gcaagatagc tggcacgctg atgcaggtcg gtgctccagt tgccaattac
      901 atctttgctc tcacctgatt ccttgtaaac cttctccatt gcactgtcgt cgtgctgatt
      961 tgtatggaga ccactttgat ctacagggcc ttcactgccc aatggcatcc agggtgagtc
     1021 tttagcgata ccctgtacat gtgcgacatt aagtcctaca gaaattgcac tgccatggtc
     1081 gcaccgaact ttgcctggcg tgtcggagcc tgcccccaca ccgttgccgc caaaagttga
     1141 tgaagcaggc gtataaaaaa catcacccca ccaagcccct gtagtaatga agtttggatc
     1201 attatcaaaa gattccttat ggtaatctcc aaccagctcc gaattcccgt catttacagc
     1261 ccaactattg cgcaacaacc acgaaccgga cgatgccttg catgtgtctg tcaaatcggt
     1321 atatcgactc cgggcgggga aactggttga gcctttcggg ggtattggct gcgctccgtg
     1381 gtatttacta ccatcaaaaa ccttgacatc gcctgacacc aggtagtcag ttatcggtgt
     1441 aaaaagctcg ttgctggata ggtgggtatc ggcccagtta ctatgggcat aaaagtcctg
     1501 caacgtatgt atagccttcc ccaacatcat acgcgctgcg gcttttttgg tagtcgcaac
     1561 tgtgtaatct gtcgtggttg atgcttccga ggcaaggttg attgcgtaaa ttgcatgtgc
     1621 tctgatagcg tctgtgctgc cctgaatgtt gtcattgtca aagtgtccat cgatgtcact
     1681 gagatcagac gtcacattga agcacaatgt gccctgatgc caggtcacta aatccccaga
     1741 agtcacggta gggcgtgcat ctttgtaaac aggtatattt ttatcattac tacaatccac
     1801 tagaggcccc caaggccccc tatagattga accatctatt aagagatgcc cgaaaagatc
     1861 tcgactctgc acaccgtaga taatccactc taacgcatcg tcagaaacaa aactatcagg
     1921 tccattgacc caataaaagg aaaaacgatc caccgtcagg cggtccccaa aagaatagcc
     1981 catcttttcg cccagcacac ccttagtgat catggtgtgt ccccatagct cagacgcagg
     2041 tttaaaggcc tgggctagag ggacaaatag caaaaatgtc aggaagatca ttaccaaggc
     2101 agcggttttt gaaatgaaac gcatgttgta ctcccgcttt ttaatattga caaatcgccg
     2161 tagccagtgg cccaaccaca cggtgattgc ccaagtcaac ggtcgggtct gttgtgccat
     2221 cgcctgccgc agcaatggca tcgcgcacct cggcctcggt cgtcggaccg ccttggcgcg
     2281 gaataagtgg actgcgcgtc agtgaagtga aacaactcga ctgcaccagc gcgtagctgt
     2341 tctcaaccgc cagttttgca ataccaggca tgcgcaccac cccaaagcgg ccactggtgc
     2401 aattcacgtt gtaggtctgc gtgatgttgg cctgattgga tgcgatggac gtagacattg
     2461 ccccgctcaa atttgccctt tgaattttcc atacctgact caatgtgcat gcattggcgg
     2521 cgacggtggc acaagaaaca gtagcgccac ctgccacact gcaagtccag gcaaaagacg
     2581 agcccgtttg acggagcgct gaagcgctgc caatgctaca aaattcagtc gcgtccgtcg
     2641 gtagataata ttcgggatag gcttgaccat tggcgctgcc gcatgctgca gcaccagcaa
     2701 gactaaacgt cgcagccacc gacttcgccg catccatcgt cacctggcag atccgggttg
     2761 aatcggcatc ggcattggtg caagcacccg ccgtccagcc ggtgaaggtg ctgccagatg
     2821 ccggtgcagc ggtcagcgtg acaaccgagc cagtgttgaa cgaagcgctg caggtgctgc
     2881 cgcagtcaat gccagcggga gcgctgatga cggtgccgga gccagttcca ttgcctgtga
     2941 cggtgagtga ccattgattg ccgggatacg tcactggcac cgaaatcagc gtccccgagg
     3001 cctggttgcc ctgcgggtct ttggcttcca ccttcacata cagggtgcgc gtgccgttgg
     3061 caaacaaggc ggcgcagccg ccagcgacgg catctacacc attgccggtg ccgatggtga
     3121 aggcgcggct gcccagactg gtgctgctgt ccacaaagca gggaaagggg tgggctgccg
     3181 cgtcagccgc cacaagcacc cgcgccaccg ccaggctgcc gccgtcaacg tcgctcaccg
     3241 taaaagcgcc accaatgccg tcgttgaact gggccgcagc aaaggcactc agcaccggtg
     3301 ccgatgtcgt caccgtcaca ttaataggct gcgcccctgg cgtggccggc gaagccgact
     3361 gaaacaacac tgtgccacct ggcgtatctt tgaccagcac gtgctttgaa cccgccacct
     3421 cgggcacgca ggcaaaagta cgctgcgtgg cgctgccgat gccaacctcg gcattgttgg
     3481 gagcgcagtc ttgcaccgta aagcccatgc ctgtcgtcag agcggtgccg gtgacggtaa
     3541 aggtggtatt gatgccaacg attgcgctgg tcggactgac cgaggttacg gttggggtgg
     3601 caggagccaa agaatcaaaa gactgtccgc cacgcaccag tcgaacatga tagccgccgt
     3661 tgccccggat gccgctgttg gcgttgcctt cttgaaaact gacgtaccac gcccagagcg
     3721 aattgacggc accgggcgaa cccgaccaaa agtaaccact cggcgtggcg gggaaaaacg
     3781 catcgttaat ggatggactg gtacggcact cctcgaccag gcggcgcaat tccctgatat
     3841 tgggcaggcg ccagtcgctc tgtccggcaa agcggctgtt gcccgccagc gccaacgcct
     3901 ctgcccaggt gtgcccagtg gcagtgccgg tgcaggtggc accattccag ctctgccctt
     3961 cgctacatcg cttccagacc aagccggtgg gtatatgcgt gacggtgttg tcctgattga
     4021 ccgccacagt cgggtccagg ttaggcgtgt agactgaatc cgggttggac ggcgcgatac
     4081 tggccgcaca ggtcaccgcc tgggcgactg aggtgcccag agatagacac agtgccacgg
     4141 cccgtagcat cgataaatag ttcatggcaa actcccttgg ataaattcaa tggcgatggg
     4201 tgatactgac cacagcgtca cctgctgccg cgtaacgcga caaaaccaga ggtgcgagag
     4261 gtgttctggt tcatggcaaa acgcctatca ctcgtcgctg cgtaccaggc gaactaggcc
     4321 tgcattggca cggggtccat cgctgtaggc gtagccattg tcgaaattga cgtaccacgc
     4381 gttgttcgaa ctgtaggcat cgggcgaacc cgaccaaaaa cccgcactct gtgtatcgga
     4441 aaaataaagc cattcaactg acggatttgt gcgcccgtaa ttaactatgc tttccagttc
     4501 tttcactgtg ggcatgcgcc agtcactggc cccgcacagc ccgatggcat tgacatcggc
     4561 ggtgtatttt tcggtatcgc agctcccgct ggcaaaacag gtgccgcctg aggcggtgcc
     4621 cacgccaccg ccattggcgg cagggtttct gttgtaccag gtgtaggtgt ggctcaggct
     4681 gcgcaggccg ctgctggttt tgatttccca gatcaggccg gtgacgttgt ctttggtgca
     4741 ggcccactcg cctggcccgc tgcccggccc ggcagtggcg ggcaacacac tgccgttatt
     4801 ggcgattttg gtgaagtcaa agcccgcgcc gccgccgcca attttgctga gttggcccgc
     4861 tgcggcctgt gcatcgcgcc cgtagcgggc atcttggcgc ggaaaagcgc cgccatcatc
     4921 cgccacggtt gcgcagttgg cggtgttggt tgaggcatca ccgcaaaaag tgatgccggt
     4981 gtcgttgagc gcaccggctg cggcttgggc caggctggta cacagcagca agggcgacag
     5041 actggcaagc agcaggcgcg ctgcgccaga ccggggtgac ggattggaca tgaattcagg
     5101 ctccagaatg aaaaaaccgc ccggcgctat agagcgaagg gcggttgggt ttgccgggca
     5161 gcagttgatg gaagtgtctt gtgggggtgc cgcaaaggca aggtgtcgcg gctgacaccg
     5221 ctcccacaga aaaccttttg gcttggcttg gcttggcttg gcttggcttg gcttggcttg
     5281 gcttggcttg gcaagacaat atcggggaaa cgaggacttt tggcattgtt tttttaaaaa
     5341 ttttgcccgt acctccatca gggcttatgc actttatcct aaaagcggct cagcgcgtat
     5401 cccccgtttg ggggatattg gggctctaaa caacggtatt gactattttt tgcgctaaac
     5461 cagcaagaaa aaagcgctac atcaccccgt tgggggttgg gtaaatttag ccggattaga
     5521 gtttggaagg ttaatttcgt aacgcttaca taattcaccc atcagcatag cagcaccctc
     5581 gcgggccgag tccgcaaact gtggctcagt ctcaatcagc ttgatttggc gtgccagtcc
     5641 acccagcagt gcggcagtgt tcgagctggt catcttctcc agcgacatca ggacggcctc
     5701 acgagccttg tccctgttgc gtttgacccg ctgggcaggg gtcataggcg agcttcccat
     5761 gctgggccta ccgactcttt taggttcatt catcgcatca ccatattacg ttacagtaac
     5821 tattttagta tagttactat aacgtaatgt gaaaattttg agttgtttga aattggcagg
     5881 acgcaaggca ggggtcgatt cttatccccg tttgttgggt ctaagggtgt ggaaaacatc
     5941 agggataaac ctgcaaagcg ttgctggggt tgagtttgcc tggtgtgctc aaaaaagtga
     6001 cgcagggctt gttcgggcat gggcaaatgt caatccaagg gatgtttgtt tgacactccg
     6061 ccagcaggcc aacttcacat aaccccccgt cgtgtcaact ggtgctttta gcctttgtct
     6121 cacccttgtg gtgctgaatc gaattcaaca tggcatcccg caatatggcg ttgatccggg
     6181 tctgataccc cttgccacgg ctttttagcc ataacaaaac atcagaatcg acccgtaccg
     6241 ttgtgacttg tttaactggc ttgtaaaacg gattacgaac agcgttcttc caaaaatcat
     6301 cgcctaatgg tggaatgtcg ctgtaatcaa tttcgctgtc tggtttgttg gccaatgcgc
     6361 tcaaacgcac ccgctgctca tcagtcaacg gcggtagatt ttgcaagtca atttcttttt
     6421 taataatctt tgttttcata acgttttcgc tccttacggt cagcagttcg agctgaaata
     6481 attcggataa cttcaatatc atcttgcgtt tcactgaagg tatgcgccac cagtaacata
     6541 aggtgacctt ctaccattcc aatcgtttgc caacgcaact caccgccttc aattcggtct
     6601 tgcttcatga cggcaaacgg gtctgcaaac accccgacag catcgtcaaa ggcgacaccg
     6661 tgcttgcgta aattgcgcaa agccttggct tcatcccatt caaatctgat tggcatctaa
     6721 ttttgttaat acaattatgt aaacttttaa ccattatgca tctgccaatg tgggtttgca
     6781 agccctgccc tagccctgcc ctgcctattg aatgagatga accgcgacat ttgggttgaa
     6841 ccacaaaccg ccgaaatttt ccgctccgtc attttgtagg tattgccaac gagatgttcg
     6901 ccgacacggt ggcaaggaag gcaaaaacgc attcgatttc agaaggcgac tgctctattc
     6961 attgggctgg ccaccgtgtt tgttggggac ttctgaccgg gcacccatct cgccaccgac
     7021 atatccacag gcgtagctcc ctatgaaagc tcacgttaaa actgtatttt gaaaatttca
     7081 caatgttgac aatgacttag gagttttaaa acataactca catgtgtact tttgaaacta
     7141 actcttgtat acagttggtt gtttaaatta tctttaagaa tcaacaactt aactaacgtt
     7201 ttcggagctt tcatagggag ctacgaccac ccaacgtgag cttctatagg gagctagcga
     7261 ccttaaatgt gagctttcat agggagctac ggcttaatgt gagctttcat agggagctac
     7321 gcttgtggat aactccaaac aaatgctcaa tctccgtcta acgtgagttc ctatagggag
     7381 ttaccgttcg atttcatgtc aatacgtgag ctttcatagg gagctagcgc gggtaggtga
     7441 gctttcatag ggagctacga ccacccaacg tgagcttcta tagggagcta cagatttgca
     7501 gccggaaaac tgtccattga gtaacgtgaa atgaggtatt cacaatagta gttcggctaa
     7561 catacgtgaa acgcacattt cacaatgaaa acaaggccgt ctgaagtgct taccgtcgtc
     7621 gaacctgaca aggagtttcg caaagcgaac aacgctgtca gcgtaagggt gaaatccggc
     7681 ccgactttgt caatgatggg tcgccggttg ttcaacgtca tgttgtatca cgcgcaacac
     7741 cttggtgcgc ctggtgtcaa tgccccggag ccctggggtg cgtgcccgaa cccaggtgat
     7801 tactactgga ttcctttgcc ggatatcgcg cacgatgccg catggggttc caaagatcac
     7861 aagcttgtcg tctggaccct tcagcagctc caaacgacgt tggtggagtc agatgacccc
     7921 tcaggccggt tctcgtctgt gcaattaatc gggtctgtgc acattgtcaa aggttctggc
     7981 cgaaggccaa gcctggtcgg atgggagttc cccaggagca cacgtgacat cctgagtaat
     8041 ccggattttt ataccaagct gtccattttt cacctgacat cgttgcaaac gaccggcggt
     8101 gctgcactct acgaaattgc aaaacgctat ctcaccaata ttggcggcaa gacaagtaaa
     8161 aatcactggc actggtggca tgacacgctg accggtcgcc aaatcggatc gattccatat
     8221 ccggagtacc gttacttcaa acgcgacgtg ttaacgccgg ccattgaaga agtgaaccgt
     8281 actgacattc atgtcgtccc cactgaatcc agacaggggc gtctggtaac ggatttgcag
     8341 tttatggtca ccttggtcac gcagacatcg ctagagttgc cgccggggcc tgtaatcgac
     8401 tctgcgctac ttgaacgagt gtgcgcgctt ggattcaccg aacgacaggc atgcgagctg
     8461 ctggggacga tggctgatga ttttttgcgc gcaacgctag agttggtcga agagcgtttg
     8521 aatgacaagt ccttggagcc tttgaaatca cccgcagcgt ttttccgtaa agcgctcaag
     8581 gacgattacg tctcgactgc caaaattaag ccgaaagtcc tgccggacaa gcctataccg
     8641 gcgaaggtag ttgaggtcga gtcgcccgca gcaactgagc gcaaaggccg ggttactcaa
     8701 gcgttactgg aattcgacgc gctggctgca atggaacaga acaatgtttt gggtgagttt
     8761 tatctgacat cagcgactca caagcggtta aagccaggcg gaacgatgtt cagaaagact
     8821 ctcggtaatt ggcttgttga tgccacggat aggcctttgg ctcagttgtt gccgtcgaat
     8881 tgagggatgt tttttcggca aaggcttaca tggcgttgat gcggtttaaa gctttgactt
     8941 acaaaaataa tgtgatttga tgtgatgcaa tataatgtga tgtcacatca cattaggggt
     9001 agcatgatta ttctgatcgg cggtgaaaag ggtggttctg gtaagagcac aacggcgaca
     9061 aaccttgcgg tttatctaat gctagagggc cacgatgtca tgttgttgga ttgcgataag
     9121 caaggtacag caaccaaatg ggcaacgcgg cgtaatgagg caggttataa ggaagtccac
     9181 agcgcccaaa agttggggga cgtttacaag acagcaatag acttaagcca gcgttatgga
     9241 cacgtcatca ttgacgctgg tgggcgtgat tcacgtgaat tgcgaactgg aatggtagcg
     9301 gctgatctga tctatatacc gcttaaagcc agtcaagctg atcttgaaac cctaccagtt
     9361 gttgacgaac ttatcacgct gtcacgtgcc atgaatccaa acgtcaaggc aagaacgctg
     9421 ctttgcatgg caccaacaaa tgatcgcatc aaagaggttg aggatgctcg ggcgttgttg
     9481 gctaattttc cagatctccc attatccgag tgcatcatcc gtgagcgcaa aatttaccgg
     9541 gatgccctgt tgcttgggcg aggcgttgtt gaaatgaaaa acagcgaagc gcgtgctgaa
     9601 attcaattgt ttggacaaga ggttttgcaa tgagtggatt taaggtcgtt ccaaagaagc
     9661 ctgctgttga cccagatgtg ttggccaaat ttgcctctgg ggccgacaaa atacaaggcg
     9721 ccatgccgtg ggaaggatta gatgacaagc gaaggatggc agcattttca ctgcgcttaa
     9781 cggcttgcga gttggccaaa ctcaaacaca ttgcgagtac cacaccagac tcaatgcacg
     9841 aattttgcat ccgagcggta aaaaaagccc tggatgaaaa aatagtgtga tatcaaatta
     9901 catcacatcg tgtcatgtcg tgtgatgcta ataaattcat aaagttcata tcaacagact
     9961 gacaaacttc ttcatgcgcg agggattgga gctgaagtag cgttgggtgg tggcaatgga
    10021 ggtgtgcccg agcagctcct tgatggcaaa aacgtccgcc cctgtctcga tcaagtgggt
    10081 ggcaaaggag cgccgcccgg agtgcgagga gccgacaacg cctgcccttt ggtacatgat
    10141 ggccaagaga cgctgcatgg tgttgggcga gaacgccccg ccgcgctggg agagaaacag
    10201 gggagcgtcc atgtagcgca ggccccggct ggccaagtag gtggcaagga tctgacgcga
    10261 aggcacatgg gtcaaataga cctcgcgaaa cttttggccc ttggtcatgc aagacagcag
    10321 gcgcaccgac tcccgaacct ccctggtctg ggggtcatag aggtcgccga tggtgagttt
    10381 ggccagctcc attgcgcgca gccccatgta atgcgagagc atcaggatgg actgattgcg
    10441 tgtggcgtgg atgcctgtct gcgctgccag cgtggccgca agctctgcca ggccgatata
    10501 aggggctttg ccttgcttca tgcccaatgg taaacgctca actgcccttg taaattagct
    10561 tatgccctaa aatgactctt ttagggcatt tattagagtt tcgttagcgt aacaatattt
    10621 ggctcgcctt caaaattggc gcatcgcctt cgggcatctg gggggcaagt catccggcaa
    10681 ctgggggttg tacgccggaa ccccagaaaa ttccgtcgtt cccaatccag cccggtcagc
    10741 agggtgacac ggctggctgt gccaggaaaa tcgctgattt ggacagcatg atgctgacat
    10801 cacgggcaca agccgatggt gctctggcaa tcgcggtaga gacacccatt actgagcgcc
    10861 ccccgcacag atcactgacg cagatccgta aggcatagca cttcaccttc tcaaccacct
    10921 gcacggtggg tcacaagctc accttcaagc ggccaaacgg caagtgcttc tcaaccacct
    10981 gcacggtggg tcacggcgag cgccgggaat acgtgcgaga caagctcttc tcaaccacct
    11041 gcacggtggg tcacacacca gagcagcgca caccagcggt ttgcttcttc tcaaccacct
    11101 gcacggtggg tcacaatagt ggcacttgcc cgtcctgcac ttctaacttc tcaaccacct
    11161 gcacggtggg tcacctagat aaataaaaca ggtaatcccc cggctttgcc ggggcgacag
    11221 taggggtttt a
//
LOCUS       DAOSLL010000068        11221 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5743_length_11221_cov_3.991492, whole genome shotgun sequence.
ACCESSION   DAOSLL010000068 DAOSLL010000000
VERSION     DAOSLL010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11221
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5743_length_11221_cov_3.991492"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /gene="phbB"
                     /locus_tag="PLB25_04525"
     CDS             <1..643
                     /gene="phbB"
                     /locus_tag="PLB25_04525"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HOE40882.1"
                     /translation="PELDKKDAWVAEMEAAGFKGMLCVAGDITDIDSCAKMVAEGEAL
                     AGGPIDILVNNAGITRDRMFGKMDKGQWDAVMSTNLTSLFNMTKQVSTKMADRGWGRI
                     INMSSLNGVKGQAGQSNYAAAKAGVIGFTKSLAQELANKGVTVNAIAPGYVGTKMVMA
                     IREDVLKGIVDAIPMKRLCKPEEIAGACSYLASDLAAYMTGATLNINGGAYYA"
     gene            832..1233
                     /locus_tag="PLB25_04530"
     CDS             832..1233
                     /locus_tag="PLB25_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40883.1"
                     /translation="MPSRDLIQFLNHPCRCYLALPCVCNVCGKLPRDECFGNAMRCML
                     KPRRPAPNYPPLPAIEVILFHRTDRTGPGATKLDDADKSSHQCYGKLLRRGNLTVPNQ
                     AHWHVAGKTLMAIGKSRKQTQRYSSELPDGG"
     gene            complement(1586..1753)
                     /locus_tag="PLB25_04535"
     CDS             complement(1586..1753)
                     /locus_tag="PLB25_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40884.1"
                     /translation="MAKEFRTEADRKATPRPLAPKGVCFTAPHGQKRSLERGVATNSK
                     KNPGKRCHQGG"
     gene            1846..3564
                     /locus_tag="PLB25_04540"
     CDS             1846..3564
                     /locus_tag="PLB25_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOE40885.1"
                     /translation="MTAVAESPVKPSVPDTRTVQLGNLLVSMGKLSARDMDRAVSAQT
                     EAGGLLPAILVNLGLVSELDVAQAQAKLLGVPFVSADELPALAPELEGVQPDFLRTHH
                     VYPMRLDDTGLTVAMAEPDDAFVIKALGLATGLAIHPCVALGSDIARAMTAAEEPHED
                     EGNSAFEADSGDFVEHLKDLASEAPVIRMVNQIIGRVIDLRASDIHLEPFEDGLHVRY
                     RVDGVIASADVIAPSLSPAVNSRVKLMAHLDIAERRLPQDGRIKTRVKGRELDLRVST
                     VPTVHGESLVMRVLDRSSVRYSMESIGFAPDNLVHFNHLLSRPHGILLITGPTGSGKT
                     TTLYTALSKLDADAHKIITVEEPVEYQLAGINQIQVHQQIGLSFANALRSILRQDPDI
                     IMIGEMRDGETAQIAVQSALTGHLVLSTLHTNTAAGAIVRLQDMGIESYLITSSVNGV
                     LSQRLVRRLCEHCKAPYTPEPSLVVSSGLQRMGLHPQQLYKSVGCEHCRQTGYAGRCG
                     IHELLVIDDPMRRAILDGLDAAALHAKATSSGMHTLYEDGLLKVAAGITSLDELLLVT
                     EDRTDA"
     gene            3557..4798
                     /locus_tag="PLB25_04545"
     CDS             3557..4798
                     /locus_tag="PLB25_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOE40886.1"
                     /translation="MPDFDWKAAQADAKVIEGRLHAASQAQVMQQLRAQGLTPLQISE
                     AGAGAPVLASVATTPLRRGGLGQSKGITQADILELTSELSIMLKAGLALDNALRVLIG
                     MNAKPAMVGLTQSILDDVKSGVPLSRALGRHPQQFGDFYINMIRSGEAGGQISAVLER
                     LFEHLDRLRALRESVISAAIYPAILLTVALLSLIAMLGFVVPQFETLFKDMGDALPLP
                     TKFIMVIGHAFTNYGLFMFFGAIVLLWLLRHWWRSPSGIRWWQARALRLPLIGKLVLK
                     YNLTLFARSLGTLLGNGVPLITALNIATQTVGNISMRQSLEGVAPKVKGGGKMVDALQ
                     ACGIFEPLAVNLVRVGEETGRMGPMLLELARIFNGDVETGIKRSLTLLEPVLILVLGI
                     LIASIIVSIMLGILSVNDLAV"
     gene            4990..6060
                     /locus_tag="PLB25_04550"
     CDS             4990..6060
                     /locus_tag="PLB25_04550"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOE40887.1"
                     /translation="MIKIKFLARPKITLADKLKEHQGVGPGFDAIRVYLSITIMALHS
                     LTLAKGVTAYVASPPWIHVLAMSLLPAFFGLGGFLVTGSGIRVKSVTKFIWHRILRIF
                     PALALEVFLSAIFLGAIFTTLNLSDYFSSSEFWIYFKNIIGIINFNLPGVFNDNPFPG
                     TVNGNLWTLRPDYYGYLILTCLMLFGIFDRKKLYFYCLLVGLLISLGLDFTYEVGKPN
                     FVPMDSTLVFSFLFGSLIFLKSDIIPWRLDIVLFCLVFYVFFGQDGGFSYLTSAMVVY
                     AIVYVGMLKIPMPAFLKRGDYSYGIYLYGFPIQQALVATFLWTREWYIVFPISAILTL
                     TLAVMSWHFIEKPFLKLKYLIR"
     gene            6261..6707
                     /gene="gspG"
                     /locus_tag="PLB25_04555"
     CDS             6261..6707
                     /gene="gspG"
                     /locus_tag="PLB25_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013696237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HOE40888.1"
                     /translation="MNHSSAPNKLYLKRRSSGFTLIELLVVLVILTLLAGLVGPRVMN
                     QLGGAKSKTAALQIADLEKSLELFKLDVGRYPATAEGLDALLNRPASANGWNGPYIKG
                     NVPNDPWGKPYVFSSPASNGGVDIMTLGADGAAGGTGENADIRNHP"
     gene            6682..7194
                     /locus_tag="PLB25_04560"
     CDS             6682..7194
                     /locus_tag="PLB25_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HOE40889.1"
                     /translation="MLTFAITPETLQGPACSYRPSLLQRGFTLIELMVVLAIGAMLVG
                     LVPFAFGKLNEGSQYRDTVRSMVTDLRQARQMAGALGQTVVYQVDLNQRQFGIEGRPQ
                     KSLPKSLEIKTIVGQLGVAPDQGLARIAFLPEGGSTGGTIELVRPSGAGVRIRVDWLL
                     GQVTQEPRSP"
     gene            7191..7613
                     /locus_tag="PLB25_04565"
     CDS             7191..7613
                     /locus_tag="PLB25_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOE40890.1"
                     /translation="MTHRLPSSRLTRQRGFSLLELLVAFAIMAMSVGLLYRAAGGSAR
                     STAEIGLQQQSVWLAESILASRNFVGIEGWNELGESAGFQWQVHSVPFASGIQGPQVV
                     PLHQVNLVVSWATGTRRGQIELATLLPERKPRPGEVIR"
     gene            7610..8269
                     /locus_tag="PLB25_04570"
     CDS             7610..8269
                     /locus_tag="PLB25_04570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOE40891.1"
                     /translation="MKTVRGFTLLELIVVMALLSLLMTGLMSALRTMAQTETKIDQRL
                     ERVDALRTTRALLARVLGGISVARIDMPSDLGKTMMPFHATANSVIWVGILPARPDVG
                     GRRYFRLAVERVDDGEALVLRLAPCDAEFNLPDWSSAEPHVLTHQVAKLTVEAQGQLP
                     QGHEQEKIWSSGWQTGWPIADVTPERVRLSLQDGAQTMLAQWTFPLYVLPLSDETINT
                     P"
     gene            8247..9143
                     /locus_tag="PLB25_04575"
     CDS             8247..9143
                     /locus_tag="PLB25_04575"
                     /inference="COORDINATES: protein motif:HMM:NF015867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspK"
                     /protein_id="HOE40892.1"
                     /translation="MKPSIRPKLGQLQPVGSARLGLCRGMALIAVLWLVAAMSLIISG
                     VVRSVRVDAQAVGLQRQLLRASVVGDAALLLAMQNLHADPKGLPKGQKNLSVDFGGQS
                     FPVRITPLNGLVDLNNAPVALLAGLYQYAGGADPATAQTLAQDTLVTRQKINRKGLPW
                     GFAAADDLLLVPGMSYDLYAKVAPLVTAVIKNGGGRVNPLAAPLGVLEALAAGNTTRA
                     NQFMAQRDAADFLLDGTFFKPELIDMAVSKSLRFEVSVELPDGSRLLRAWQVYWDTDP
                     RTNLPWRVLGKQQLLLPSPRAV"
     gene            9162..10472
                     /locus_tag="PLB25_04580"
     CDS             9162..10472
                     /locus_tag="PLB25_04580"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOE40893.1"
                     /translation="MSTASTATQFFGLDLASLRRDLLIAWAKMLNWPVLAWLWPKLSV
                     RVWLPDGKSVLSRGPGTPCVDDPILARSARFDAVQMPEHLLLRKTLQMPQLQAHELQA
                     ALTLQVSGLNPFAPDDLIWTNALEPDRERGAFLTVHLVMASRKQLARYIEQAHPGLNP
                     AKTEVWVAGLPEQTHLVLPGFGESVRASHSVVWRWVSALLLLLVLALVAAIGVTPSVQ
                     LYLRALQAHAAINDLQQRAIPVVKQREALVHVTDQLANLAEAMGKTVPPLQALKLISD
                     ALPDDTSLLNLQVQGLKVTMSGQTSNASVLMRQLGATPGLRDVTAPTPAVKPPGIQRE
                     QFTIEFKIDAAQIAPAPVASVPIVPAPVISAPVVVVPIAPAPIASAASVPARIASAPS
                     TSTPSVSMPMAVGPSVPARIASAPRGPTRVGSAPLVPAPIAPAK"
     gene            10469..11023
                     /gene="gspM"
                     /locus_tag="PLB25_04585"
     CDS             10469..11023
                     /gene="gspM"
                     /locus_tag="PLB25_04585"
                     /inference="COORDINATES: protein motif:HMM:NF040576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HOE40894.1"
                     /translation="MNRFSLDRIALLQVLTVLALLLPVLGSAYVVWVKHQRVEGILAE
                     IEPRHARLQGLMARSADLQAFGAKASAQLSQLTYPATQDATKSGNDAQQRIRALFSDS
                     KLDIISIQVLPAKEVGKFDRIGISLRVEGNLSAIQAALSRLPEQMPVVVVEGMTLQTI
                     GAVLPASIQRLGGQFNFSVFRVRP"
     gene            11020..>11221
                     /locus_tag="PLB25_04590"
     CDS             11020..>11221
                     /locus_tag="PLB25_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40895.1"
                     /translation="MRLTPVRLLLLLVLGLGAGLLSLWLDQQGHWRNITWAVPAAKAP
                     DIKAPVDILPAAGNPAPSYASVQ"
BASE COUNT         2253 a         3248 c         3184 g         2536 t
ORIGIN      
        1 cccggagctg gataaaaaag atgcatgggt cgcggaaatg gaagcagccg gcttcaaggg
       61 catgctctgc gtggctggcg acatcaccga cattgatagc tgcgcgaaga tggtagctga
      121 gggcgaagct ttggctggcg gcccgatcga catcttggtt aacaacgccg gcatcacccg
      181 cgaccgcatg tttggcaaga tggacaaagg ccagtgggat gcagtgatga gcaccaacct
      241 gaccagcctg ttcaacatga ccaagcaggt ctcgaccaaa atggctgacc gcggctgggg
      301 ccgcatcatc aacatgtcgt ccctgaacgg tgttaaaggc caggctggac aatccaacta
      361 cgcagccgcc aaggctggcg tgatcggctt taccaagtca ctggcgcaag aactggccaa
      421 caagggtgtt accgtcaatg caatcgcccc tggctatgtc ggtaccaaga tggtcatggc
      481 gattcgcgaa gatgtgctca agggcatcgt tgacgcaatc ccgatgaagc gcctgtgcaa
      541 gcccgaggaa atcgccggtg cctgctctta cctggcctct gatttggctg cctacatgac
      601 cggtgccacg cttaacatca atggcggtgc ctattacgcc tgatccggcg cgggcctgac
      661 agcgcagccc tggggtcggt gcggcaagct tgccaactgc cgcacagcgc cttgtgcccc
      721 aggttacgcg gttgacaacc caataagcgg gtgcgcaact tgacggttgc gcacccgttt
      781 tgcgtttttc cagccagtct tgcggaccga atcacggggc tggtacagat cgtgccaagc
      841 cgcgatctga ttcaatttct aaatcatcca tgtcgttgtt atctcgcctt gccttgcgtt
      901 tgcaatgtct gcggcaagct gcccagagat gaatgttttg gaaatgcaat gcgctgtatg
      961 ctgaagccgc gcagaccagc cccgaactac ccgcccctac cagctattga ggtcatcctt
     1021 ttccaccgca ccgaccgcac tgggcctggc gcgaccaaac ttgatgatgc cgacaaatca
     1081 agccatcaat gctatggcaa attgctcagg cggggcaatt tgacggtgcc aaatcaggcg
     1141 cattggcatg ttgctggcaa gacgttgatg gctatcggaa agagccgcaa gcagactcaa
     1201 agatattcgt cggaattgcc cgatggcggc taacgtgatt gcactgcgaa ggtcagaaaa
     1261 acacgaagcc agccacaacc gtcaagtggc caaggtaagc ggtgaacgcc aatcgaaatg
     1321 ccaataagca gcagatggat accgtgcttg cttgctcaat ggggcaggtc actaatgcgc
     1381 tagtgtaatg caggtgggca tatcgcgtca ataggaaggg gaaggggaaa ggctgatttt
     1441 gcttcaatat ctatagctag ttacgcaaca ctgtcaaggg ctagctcatc aaaacaacct
     1501 cgatttccgg taaatcgcac aggccgcaat gccgttgtcg aatcgtgcag ttgcgacaac
     1561 ggctggcggc aatcttgtgg ccggatcagc caccctggtg gcagcgcttg ccggggtttt
     1621 tcttgctgtt ggttgcgaca ccgcgctcca ggctgcgctt ttggccatgg ggtgcggtaa
     1681 aacaaacgcc cttcggggcc aacggacggg gggtggcttt gcgatcagct tcggtacgga
     1741 attctttggc catgacttag ggtcctgcaa gttgataaaa ccggcgatta tccctgcttt
     1801 gccgttacca ttgccgccat tcgacctgct taacccccgc ttttcatgac cgctgttgct
     1861 gaatcacccg tcaaaccatc cgtgcccgat acgagaaccg tgcaattggg caatctgctc
     1921 gtcagcatgg gcaagctgtc ggcccgcgac atggatcgcg ctgtttccgc gcaaaccgag
     1981 gctggcggac tgctgcctgc cattctggtc aatctggggc tggtgtccga acttgatgtg
     2041 gctcaggcac aagccaagct gctgggggtg ccttttgtgt cggcagatga attgcctgcg
     2101 ctggcccccg agctggaagg cgtgcagccc gactttttgc gcacccatca cgtttatccg
     2161 atgcgcctgg atgacaccgg gctgacggtg gccatggcag aaccagatga tgcttttgtc
     2221 atcaaggctc tgggtctggc caccggtctg gccattcacc cctgtgtggc gctgggatca
     2281 gacatcgccc gggccatgac cgctgccgag gaaccccatg aagacgaggg caattcggcc
     2341 tttgaagccg attcgggcga ctttgtcgag catctgaaag acctcgccag cgaggccccg
     2401 gtgattcgca tggtgaacca gatcattggc cgggtgattg acctgcgcgc ctctgacatt
     2461 catctggagc cattcgaaga cggcctgcat gtgcgctacc gggtggacgg tgtcatagcg
     2521 tccgccgatg tgattgcgcc cagcttaagc ccggccgtca attcacgtgt caaactgatg
     2581 gcgcatctgg acattgccga gcgccgcttg ccgcaagatg gccgcatcaa gacccgggtc
     2641 aagggtcgcg agcttgatct gcgggtatcg accgtaccca ccgtgcatgg cgaaagtctg
     2701 gtgatgcgcg tgctggaccg ctccagtgtg cgttacagca tggaatcgat cggttttgcg
     2761 ccggacaacc tggtgcattt caaccatctg ctgtcgcgcc cgcatggcat cttgctcatc
     2821 accggcccga ccggttcggg caaaaccacc accctttaca ctgcattgtc gaagctcgat
     2881 gccgacgcgc acaaaatcat cactgtcgaa gaacccgttg aataccaact ggccggcatc
     2941 aaccagattc aggtgcacca gcaaatcggc ctgagctttg ccaacgccct gcgctcgatc
     3001 ctgcggcaag accccgacat catcatgatc ggtgaaatgc gcgacggaga aactgcccaa
     3061 atagcggtgc agtccgcact gacagggcat ctcgtgctat caactttgca caccaacacg
     3121 gccgccgggg caattgtccg gctgcaggac atgggcattg agtcgtacct gatcacctcg
     3181 tcagtcaacg gtgtgctgtc gcagcggttg gtgcgccgcc tgtgcgagca ttgcaaagcg
     3241 ccctacaccc ccgagcccag cctggtcgtc agcagtggtc tgcagcgcat gggactgcac
     3301 ccgcagcagc tctacaagtc tgtcggctgc gagcattgcc gccagaccgg ctatgccggg
     3361 cgctgcggca tccatgagtt gctggtcatt gacgacccga tgcgacgcgc cattctggac
     3421 ggtctcgatg ccgccgccct gcatgccaag gccaccagtt ccggcatgca caccctgtac
     3481 gaggacggtt tgctcaaggt tgcggcgggc atcaccagcc tcgacgagct gctgctggtg
     3541 accgaggacc gcaccgatgc ctgactttga ctggaaggcc gcccaggctg atgccaaggt
     3601 catcgaaggt cgcctgcacg ccgccagcca ggcccaggtc atgcagcagt tgcgcgccca
     3661 gggcctgaca ccgttgcaaa tcagcgaagc aggcgctggc gccccagttc tggccagcgt
     3721 cgcaacaaca cccttgcgcc ggggcggact tggccagtcc aagggcatca cccaagccga
     3781 cattctggag ttgaccagcg agctatcgat catgctcaag gccggtctgg ccctggacaa
     3841 cgcgctgcgg gtgctgattg gcatgaacgc caaacccgcc atggtcggct tgacccagtc
     3901 gatcctcgac gatgtcaaaa gcggtgtgcc cctgagccgg gcgcttggcc gccaccctca
     3961 gcaatttggt gacttctaca tcaacatgat ccgctcgggc gaagcgggtg gccagatcag
     4021 tgccgtgctg gagcgtctgt ttgaacacct tgaccgactg cgtgcactgc gtgaaagcgt
     4081 catttcggcg gccatttacc cggccatatt gctcacagtt gcgttgctgt ccctgattgc
     4141 catgctgggt tttgtggtgc cccagtttga aaccctgttc aaggacatgg gcgatgccct
     4201 gccgctaccg accaaattca tcatggtcat tggccatgcc tttaccaact atggactgtt
     4261 catgtttttt ggtgcaattg tgctgctgtg gctgctgcgc cactggtggc gctctccgtc
     4321 gggaattcgt tggtggcagg cccgggcact gcgactgcca ttgattggca aactggtgct
     4381 caagtacaac ctgacgctgt ttgcccgttc gctgggcacc ttgctgggca atggtgtgcc
     4441 cttgattacc gcattgaata tcgccaccca gacggttggc aacatcagca tgcgccagtc
     4501 gctcgaaggc gttgcgccca aggtcaaggg cggcggcaag atggttgatg cgctgcaagc
     4561 ctgcggcatt tttgaacctc tggctgtcaa tctggtccgg gtcggcgaag aaaccggtcg
     4621 catgggcccg atgctgctgg agctggcccg catcttcaat ggcgacgttg aaaccggcat
     4681 caagcgcagc ctgacgctgc tcgaacccgt gctgattctg gtgcttggca tactcattgc
     4741 atcgatcatt gtgtcgatca tgctgggtat tctgtcggtc aatgatctgg cggtgtagcg
     4801 tgcgtctgct tgcagttcag cggctccatg cggtggtgtc gcgtgccagc aagtgccttc
     4861 gcgctctgcc acagcaactg ttctgacacg cctgtgacga gtcttatcga aagaacagag
     4921 agaaaagaag ggtgatttga cccgactgcg gtttttttca acaaccctct gacgggtccg
     4981 gttcttctga tgatcaaaat taaattcttg gcgcgcccga aaataaccct ggccgataaa
     5041 ctgaaagagc atcaaggtgt gggccctggt tttgatgcca ttcgggttta cttgtccatc
     5101 accatcatgg ccctgcacag tttgacgctg gccaaggggg tgaccgccta tgtagctagt
     5161 ccgccatgga ttcacgtttt ggccatgtcc ttattacctg cattttttgg tctgggcggt
     5221 ttcttggtca caggcagtgg cattcgggta aaaagcgtta ccaagtttat ctggcatcgc
     5281 atattaagaa tattcccggc attggcattg gaagtttttt tatcggctat ctttctgggt
     5341 gccatcttta cgacgttgaa tttatccgac tatttctcgt caagcgaatt ttggatttat
     5401 ttcaagaaca tcatcggcat cattaatttc aatctacccg gtgtcttcaa tgacaatcca
     5461 tttccaggaa cggtaaacgg aaacttatgg accttgagac cagattatta tggctatttg
     5521 attttgacat gtctgatgtt atttggaatt tttgaccgaa aaaaacttta tttttactgc
     5581 ctgctggttg ggcttttgat atctttggga cttgatttca catatgaggt tgggaagcca
     5641 aactttgtac ccatggattc aaccctggtt ttctcttttt tatttggtag tttgattttt
     5701 ttaaaatccg acattattcc atggcgattg gacatcgtac tgttttgtct ggttttttat
     5761 gtattttttg gacaagacgg tggcttttca tatcttacgt cagccatggt tgtgtatgcc
     5821 attgtttatg tcggaatgct gaaaattccc atgcctgcct ttctaaaacg gggagactat
     5881 tcctatggca tttatctgta cgggtttccg attcagcaag cgctggtcgc cacgtttctt
     5941 tggaccaggg agtggtatat tgtttttcct atttctgcaa ttttgacact gaccctggca
     6001 gttatgtcat ggcattttat agaaaaacca tttctgaaat taaaatattt gatccgctaa
     6061 gattgtcatt gtggccattt ttgaagttat ctgttgatta tttctgcatg cactgcttgg
     6121 ctctgtcaac tcttaacttg tgtcaggtaa aggttatgta aaggcagacc tgaacatccc
     6181 tgatcccatt tgcttgctgc gatatactat taaaaatgtt tggcttaaaa atttaacttc
     6241 agtacatgaa ttccatcacc atgaaccact cttctgcccc gaacaagttg tatctgaagc
     6301 gtcgaagcag cggctttacc ctgatcgagt tgctcgtcgt cttggtgata ttgacgctgt
     6361 tggctggtct ggtaggaccg cgcgtgatga accagcttgg tggagccaag tcgaagacgg
     6421 ccgcactgca gattgccgac cttgaaaaat cactggagct cttcaagctc gatgtcgggc
     6481 gctacccggc aacggcagag ggacttgatg cgctattgaa ccggcccgcc agtgcgaacg
     6541 gctggaacgg cccctacatc aagggcaatg tacccaacga cccctggggc aagccctatg
     6601 tcttcagcag tccggccagt aacggcggcg ttgacatcat gacccttggt gccgatggcg
     6661 cggcgggcgg cactggcgaa aatgctgaca ttcgcaatca cccctgagac acttcaaggc
     6721 cccgcttgta gctaccggcc cagtctgctg cagcgtggtt tcacgctgat cgagttgatg
     6781 gttgtgctgg ccatcggtgc catgctggtt ggtctggtgc cgttcgcatt tggcaaactc
     6841 aatgaaggca gccagtaccg ggacaccgtg cgaagcatgg tcaccgacct ccgccaggcc
     6901 cgccaaatgg caggggcact cgggcagaca gtggtttacc aagtcgatct gaaccagcgc
     6961 cagtttggca tcgaaggcag accacaaaag tcgctgccca agtctcttga aatcaagacc
     7021 atcgttggtc aactcggcgt agcgccggac cagggcctgg cacgcattgc tttcctgccc
     7081 gaaggcggct caaccggcgg caccatcgag ttggtccggc cgtcgggtgc cggcgtgcgt
     7141 attcgggttg actggttgct cgggcaagtc acccaggaac cgcgttcacc atgacccaca
     7201 ggctgccgtc atccagattg acccgacagc gaggcttttc gctgcttgaa ttattggtgg
     7261 cctttgccat catggcgatg tcggtcggcc tgctttaccg cgcggccggt ggcagcgccc
     7321 gcagcaccgc cgaaatcgga ctgcagcagc agtctgtttg gctggccgag tcgattctgg
     7381 caagccgcaa cttcgtcggc atcgagggct ggaacgaatt gggcgagtca gccgggtttc
     7441 agtggcaagt tcacagtgtg ccatttgcca gcggcattca aggacctcaa gtggtgccgc
     7501 tgcatcaggt gaacctggtg gtttcttggg ccacaggcac ccgccggggt caaatcgagc
     7561 tggcaactct gttgcctgaa cgtaagcctc gcccaggtga ggtgattcga tgaaaaccgt
     7621 gcgcggcttt accctgctgg aactgattgt ggtgatggcg ctgctgtcac tgctgatgac
     7681 cgggctgatg tccgccctgc gcaccatggc acaaaccgaa accaaaattg accaacgcct
     7741 tgaacgggtt gacgcgttgc gaacaacccg cgcgttactg gccagggtgc ttggtggcat
     7801 ttctgttgcc agaatcgaca tgcccagtga tttgggcaag accatgatgc cctttcatgc
     7861 cacggccaac agcgtgatat gggtcggcat actgccagcc cgaccggatg tcggtgggcg
     7921 gcgatacttc cgtctggccg ttgaacgagt cgatgacggt gaggcactgg tgctgcggct
     7981 ggccccctgc gatgcagaat ttaacttgcc cgactggtca agcgccgagc cgcacgtttt
     8041 gactcaccaa gttgccaagc tcactgtcga ggcgcagggg cagttgccac aaggacatga
     8101 gcaagaaaaa atctggtcat ccgggtggca aaccggttgg ccaattgccg acgtgacacc
     8161 cgagcgggtg cgcctgtcac tgcaagatgg cgcgcaaacc atgctagcac agtggacctt
     8221 tcccttgtat gtcttgccgc taagcgatga aaccatcaat acgccctaag ctcgggcaac
     8281 tacaaccggt cggttcggca cggctcgggc tctgccgcgg catggccctg attgccgttt
     8341 tgtggctggt tgcagccatg agtctgatca tctcaggggt tgtccggtca gtccgcgttg
     8401 atgctcaagc ggtcggcttg cagcgccagt tgctgcgggc aagcgtcgtg ggcgacgcgg
     8461 ccctgctgct ggccatgcaa aacctgcacg cagaccccaa agggctgccc aaaggccaga
     8521 aaaacctgtc agttgacttt ggcggacaaa gttttccggt tcgaatcaca ccgctcaatg
     8581 gactggtgga cctgaacaac gcgccggtgg cattgctggc gggcctatac cagtacgctg
     8641 gcggcgccga cccggcgact gcgcagacgc tggcacaaga cacgctagtc acccgccaga
     8701 aaatcaaccg caaaggcctt ccctgggggt ttgccgccgc cgatgacctg ttactggtgc
     8761 ccggcatgag ctatgacctc tatgctaaag tagcacccct tgtcacggct gtcatcaaaa
     8821 acggcggcgg acgcgtcaat cccctggctg cgccactggg cgttctggaa gcattggcgg
     8881 ccggcaatac cacccgcgca aatcaattca tggcacagcg cgacgctgcc gactttttgt
     8941 tggatggcac tttttttaaa cctgagttga tagacatggc agtatccaaa agcttgagat
     9001 ttgaagtcag cgttgaactg cctgacggct cccgtttgct gcgggcctgg caggtctatt
     9061 gggacaccga tccgcgcaca aaccttccat ggcgggtgct aggcaagcaa caattgcttt
     9121 tgccaagccc gcgcgcagtc tgattgccta cctgcctgac aatgtccact gcatcaaccg
     9181 ccacccagtt ttttggtctt gatctcgcgt ccctgaggcg tgatctgttg attgcctggg
     9241 ccaaaatgct caactggcca gtgctggcct ggctttggcc caagttgtcg gtgcgggtat
     9301 ggctacccga cggcaaatcc gttttgagtc gcggccccgg taccccttgc gttgatgatc
     9361 cgatactggc ccggtcggcg cgctttgacg cggtgcaaat gccagagcac ctgctgctgc
     9421 gcaagacgct gcagatgccg cagcttcaag cacacgagct gcaggcggcg ctgaccctgc
     9481 aggtgtctgg cctcaaccca tttgccccgg acgatctgat ctggacgaat gcgcttgagc
     9541 ctgaccgcga acggggtgca tttctcaccg ttcacctggt catggcttca cgcaaacagc
     9601 ttgcccgcta tatcgaacaa gcccacccgg gcctcaatcc ggccaaaacc gaagtctggg
     9661 tggctggttt gccagagcag acccatttgg tgctgcccgg ttttggcgag tcagtgcggg
     9721 ccagtcacag cgtcgtctgg cgctgggtca gcgcgctgct gctgctgctg gtgctcgccc
     9781 tggtcgccgc cataggtgtc acacccagcg tacaactgta cctgcgggca ttgcaagccc
     9841 acgccgccat caatgacctg cagcagcgtg ccatccccgt tgtcaagcag cgtgaggcgt
     9901 tggtgcacgt caccgaccaa ctggccaact tggccgaagc catgggcaaa accgtgcctc
     9961 cgttgcaggc cttgaagctg atttccgacg cattgccgga cgacacatcg ctgctcaacc
    10021 ttcaggtcca gggtctcaaa gtgaccatgt ccggtcaaac ctcaaatgcc tccgtgctga
    10081 tgcgccaact tggcgctacc cctggcctgc gcgatgtcac cgccccgaca ccagcagtca
    10141 agccaccagg tatacagcgc gaacagttca ccatcgagtt caaaatcgac gcggcgcaga
    10201 tcgcgcccgc cccggtcgct tcggtgccca tcgttcccgc tccggtcatt tccgcacccg
    10261 tcgttgtggt gccaattgct cccgccccaa tcgcttcagc cgcaagcgtt cctgcccgaa
    10321 tcgcttctgc cccaagcact tcaaccccaa gcgtttcgat gcctatggct gtcggcccaa
    10381 gcgtccctgc ccgaatcgct tccgccccaa gggggccgac cagagtcggc tcggccccac
    10441 ttgttcccgc tccaatcgcc cccgccaaat gaatcgtttt tcgcttgatc gtattgctct
    10501 tttgcaagtg ctcaccgtac tggcgctgct gctgccggtg ttgggcagcg cgtatgtggt
    10561 gtgggtcaag caccagcggg tagagggcat tttggccgaa attgagcctc gccatgccag
    10621 gctgcagggg ctgatggccc gcagcgccga cctgcaggcc ttcggggcca aagccagtgc
    10681 gcagctttct caactgactt acccggcaac gcaagacgcg accaagtccg gcaacgatgc
    10741 ccagcaacgc atccgcgccc ttttttccga cagtaagctg gacatcatca gcattcaggt
    10801 attgccggcc aaagaagtcg gcaaattcga cagaatcggc atcagcttgc gggtcgaggg
    10861 taatttgtct gccattcaag ccgcattgag ccgcttgcca gaacaaatgc cggtggtggt
    10921 ggtggagggc atgacgctgc agaccatcgg tgcggtgctt ccagccagca ttcaacgtct
    10981 cggcgggcag tttaatttct ctgtgttcag ggtgcggcca tgaggctgac tccggtccgt
    11041 ctgctgctgc tgctggtgct gggcctgggc gccggtctgc tcagcctatg gctcgatcag
    11101 caaggccatt ggcgcaacat cacttgggcg gtgccggcgg ccaaagcgcc agacatcaag
    11161 gccccggtcg acatcttgcc tgctgctggc aaccctgcac cgtcctatgc cagcgttcag
    11221 g
//
LOCUS       DAOSLL010000069        11193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5760_length_11193_cov_4.497307, whole genome shotgun sequence.
ACCESSION   DAOSLL010000069 DAOSLL010000000
VERSION     DAOSLL010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11193
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5760_length_11193_cov_4.497307"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..433
                     /locus_tag="PLB25_04595"
     CDS             <1..433
                     /locus_tag="PLB25_04595"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE40896.1"
                     /translation="DRFGSTTYAHQSAFLVSKQFVTMGKADAAKAALTWLAEQSTDVG
                     YQALAKLRLATILMESKNLDNALSLMNSTFPASFEGLVADRKGNIMALKGDKSQAIIE
                     YEKAYRLLDARTEYRRLIEVKLNAMGVDVQGRDLLALTTGK"
     gene            440..1576
                     /gene="bamB"
                     /locus_tag="PLB25_04600"
     CDS             440..1576
                     /gene="bamB"
                     /locus_tag="PLB25_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HOE40897.1"
                     /translation="MAKYFSIRLLQVCLAGVLVLWLASCASGSSRPNPAELGSNPSLL
                     GARLVWTSNLGSVDFPLAIKANRSGVMLASADGSVVSLDARTGAAGWRANVGSPIAAG
                     VGSDGRYVAVVTRDSELVVLEAGRELWRSRLAAQVFTSPLVAGERVFVLGADRMLTTF
                     DAQSGRKLWQYQRPGEALVLRQAGVLLPVHNTLVVGFSGRLLGLNPLTGSVLWDAAIA
                     TPRGTNDIERLVDLVAGASREADVVCVRAFQAAVGCVETVRGKVIWRQAASGSVGVHG
                     DDHQVYGVEADGRVLSWSASDGKQVWETDRLRFRQLSAPLVLGRSVVVGDNSGFVHFV
                     SRVDGSLLTRLTTDGSAISVTPVSADGTLVVVTRNGGVFGFRPE"
     gene            1608..2945
                     /gene="der"
                     /locus_tag="PLB25_04605"
     CDS             1608..2945
                     /gene="der"
                     /locus_tag="PLB25_04605"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HOE40898.1"
                     /translation="MKPVIALVGRPNVGKSTLFNRLTKSRDAIVADFFGLTRDRHYGN
                     GKQGKHEFIVIDTGGFEPDAASGIYMEMAKQTRQAVAEADVVVFVVDARGGVSAQDHD
                     IANYLRRLGKPCILVANKAEGMLEGIQLVEFYELGLGEVHPVSAAHGQGIRSLIDVAL
                     EPFGALNDDVSPEFEPGVIKLAVAGRPNVGKSTLINVWLGEERLVAFDLPGTTRDAIS
                     VPFERNGQKFELIDTAGLRRRGKVFEAIEKFSVVKTLQAIESAHVVLLLIDATQGVTD
                     QDAHIAGYILENGRAVVLAINKWDAVDEYQRELVKRSIETRLGFLKFANLHLISAQKR
                     QGLGPLWVSIAQAFKAATCKMSTPVLTRLLLESVQFQSPKRAGMFRPKLRYAHQGGMN
                     PPVIVIHGNSLEHVTDAYKRFLEGRFRKEFDLAGTPLRIEMKTSSNPFSEKAD"
     gene            3064..3312
                     /gene="hfq"
                     /locus_tag="PLB25_04610"
     CDS             3064..3312
                     /gene="hfq"
                     /locus_tag="PLB25_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HOE40899.1"
                     /translation="MSNKGQLLQDPFLNTLRREHVPVSIYLVNGIKLQGQIESFDQYV
                     VLLRNTVTQMVYKHAISTIVPGRAVNFSVVEGDEPGSA"
     gene            3290..4465
                     /gene="hflX"
                     /locus_tag="PLB25_04615"
     CDS             3290..4465
                     /gene="hflX"
                     /locus_tag="PLB25_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HOE40900.1"
                     /translation="MTNLAQPDIRSAPTLLVGVDFGLPRFDDQLEELGLLAQTAGLHP
                     VARVTCKRRAPDAALYIGRGKADEIKQLALQMGVQEILFDQSLSPAQQRNLERHLELP
                     VNDRTLLILQIFGQRARSHEGKLQVELARLQYLSTRLVRRWSHLERQSGGIGMRGGPG
                     ETQIELDRRMISDSIKRTKERLVKVKRQRQTQRRQRERRETFNISLVGYTNAGKSTLF
                     NALVKARAYAADQLFATLDTTTRQFYLGEIGRMISLSDTVGFIRDLPHGLIDAFQATL
                     QEAVDADLLMHVVDFSSPNFTEQIEQVQRVLAEIGAADVPQVLVFNKLDRLEPDNRPA
                     VLQDVYELDGLPTPRVFLSARDGEGLVALRRLLSERLLPVQNLVVPADFPLGREAGV"
     gene            4505..5911
                     /gene="hflK"
                     /locus_tag="PLB25_04620"
     CDS             4505..5911
                     /gene="hflK"
                     /locus_tag="PLB25_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HOE40901.1"
                     /translation="MKLQLPTFSLAFWPQRLKGMFNLNDPRWGRSDDKPADNADSGET
                     DRPATGVPETPPRANEDRRPRQGGNQGPPDLDELWRDFNRKLGGLFGNTKVGGRGGPA
                     SGGTGGGNRTPGGFQPDMKNAGVGAGLVVAVAVLIWLGTGFFIVQEGQQAVITQFGKF
                     NSTVGAGFNWRLPYPIQRHEVIFVTQIRSVDVGRDTVIKATGLRESAMLTEDENIVEI
                     KFAVQYRLSDARAFLFESKNPADAVVQAAETAVREVVGKMRMDSALSDERDQIAPRVR
                     ALMQKILDRYKVGIEVVGINLQQGGVRPPEQVQAAFDDVLKAGQERERAKNEAQAYAN
                     DVIPRAVGAASRLKEEADAYKARIVAQAQGDSQRFKSVLAEYQKAPQVTRDRMYIDSM
                     QQIYSSVTKIMIDSRQGSNLLYLPLDKIIQMTGQTSDDAVPAPLAVPPAGAPANAAAT
                     PVDARTRDAARARTRESR"
     gene            5921..6835
                     /gene="hflC"
                     /locus_tag="PLB25_04625"
     CDS             5921..6835
                     /gene="hflC"
                     /locus_tag="PLB25_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HOE40902.1"
                     /translation="MNRLGFVISSVLFVLAIASSTLFVVDQRQFGVVFALGQIKDVIT
                     EPGLNFKLPPPFQNVSYIDKRLLTLDSTDNEPVLTAEKQRVVIDWYVRWRISEPTQYI
                     RNVGTTEAAGASQLNRVVRNAFQEEINKRTVKELLSAKREALMEDVKGEVLGQVRGAK
                     PWGVDVIDVRITRVDYVDAITESVYRRMEAERKRVANELRSTGAAEGEKIRADADRQR
                     EVTIANAYRDAQKIKGEGDAEAARTYAEAFGRDPQFAQFYRSLGAYQSTFSKKSDVMV
                     LDPSSSEFFKALRGGAAGASVAPAQGKK"
     gene            6850..7038
                     /locus_tag="PLB25_04630"
     CDS             6850..7038
                     /locus_tag="PLB25_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HOE40903.1"
                     /translation="MDSTTFWLAVALVLVIEGFMPFLSPATWRKTFLQVLQLGDGQLR
                     FFGLCSLLLGVALIWWLA"
     gene            7096..8244
                     /locus_tag="PLB25_04635"
     CDS             7096..8244
                     /locus_tag="PLB25_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HOE40904.1"
                     /translation="MSAWVLPDHIADVLPSDARHIEELRRDLLDTARCYGYELVMPPL
                     LEHIQSLLTGAGVALDLQTFKLVDQLSGRMMGLRADSTPQVARIDAHLLNRSGVTRLC
                     YCGPVLHTRPSAPHATREPLQFGAEIYGHAGLEADIEVLTLALDCLKAAKVQAPSIDL
                     ADARLVKAMLSGMSVNAAELANLYAALAAKDSPELITLTQHMPAEVRQGLLALVQLYG
                     DDHVLIEAEKVLQPLPAVREALLSLKQLANHLQGVQVTFDLADLRGYAYYTGARFAIY
                     APAGSDALVRGGRYDEVGAVFGRNRPAAGFSLDLKALVAAVQPRPLQVAIRAPWRDDI
                     ALADAIAALRNRGEIVMRVLPGHESEVDEFHCDRKLIEVAGQWVVQAI"
     gene            8262..9638
                     /locus_tag="PLB25_04640"
     CDS             8262..9638
                     /locus_tag="PLB25_04640"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HOE40905.1"
                     /translation="MTAIKGRNVVVVGTQWGDEGKGKLVDWLTESAQGVVRFQGGHNA
                     GHTIVINGVKTALHLIPSGIMRPGVKCYIGNGVVLSAAKLFEEIAGLEKVGVELRSRL
                     RISEACPLILPFHAALDVAREAAREQGGIEKIGTTGRGIGPAYEDKIARRALRVQDLK
                     FPERFASKLHDLLDLHNHVLTTYLGSRAFDFGPMLAPYMKDGTVQFQPVYDEAMRHAE
                     LLKPMMADVSRELNNAHLKGENLLFEGAQGTLLDVDHGTYPFVTSSNCVAGNAAAGAG
                     VGPGLLHYILGITKAYCTRVGGGPFPTELDWERPGTPGYHLSTVGAEKGVTTGRSRRC
                     GWFDAALLKRSAQVNGLTGLCITKLDVLDGLKELLLCTGYELDGDLTDILPMGADDIA
                     RCKPVYETLAGWSDSTVGVTEYDKLPSAARLYLQRIEQVTGVPIHMVSTSPDRDHTIL
                     MRHPYLAD"
     gene            9735..10259
                     /locus_tag="PLB25_04645"
     CDS             9735..10259
                     /locus_tag="PLB25_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HOE40906.1"
                     /translation="MLTEDGKHLYVSYDEYNNLIEKLAIKVFQSGWQFDTILCLARGG
                     MRPGDVLSRVFDKPLAIMSTSSYRSDAGTVQGKLDIAHYITTPKGEIAGRVLLVDDLA
                     DSGLTLNAVIQQLKNNYKPIEELRSAVIWTKALSTFTPDYCVDYLPTNPWIHQPFENY
                     DSMRPEQLIQKWSV"
     gene            10260..>11193
                     /locus_tag="PLB25_04650"
     CDS             10260..>11193
                     /locus_tag="PLB25_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOE40907.1"
                     /translation="MSSDHAKAISTALIHHSYTPPAGFAAPQSAVFKASTVFFPNVAA
                     MRRFEWKDKTAYTYGLHGTPTTYTLEERLCTLEGGRQCLLLPSGLAAIAAVSLALLKS
                     GDEVLLPDNVYSPSKALAQGELANWGISHRFYDAMRPHDLAAKIGPNTRLVWLEAAGS
                     VTLEFPDLCEQVRICKAAGVLCALDNTWGAGLAFKPFDLHPDNRSALAVDISIHALTK
                     YPSGGGDVLMGSVITVDQALHMKLKLCHMRLGQGVGANDAEAVLRGLPSLALRYQAHD
                     EATRALAQWSLTRSEFAQVLHPALPQSPGHGQWSR"
BASE COUNT         2333 a         2955 c         3359 g         2546 t
ORIGIN      
        1 agatcgtttc ggttctacca cctatgctca ccagtctgcg tttttggtgt ccaaacaatt
       61 cgtgaccatg ggtaaggccg atgcggccaa ggcagcactc acctggcttg ctgaacaatc
      121 aaccgatgtc ggctaccagg ctttggccaa gctccgattg gctaccattt tgatggaatc
      181 gaaaaatctc gataatgcct tgagtttgat gaatagcact ttccccgcca gttttgaagg
      241 cctcgtcgct gaccgcaagg gtaacatcat ggctttaaaa ggggacaagt ctcaagccat
      301 cattgagtat gagaaggctt accgtctgct cgatgctcgc acagaatatc gtcgtctgat
      361 cgaggtcaag ctcaacgcca tgggggttga tgtgcaaggt cgtgatctgt tggcattgac
      421 cacaggaaag taatcaccaa tggccaaata tttctcgatt cggttgttgc aagtctgctt
      481 ggcgggagta ttggtgctgt ggcttgcctc ctgtgccagt gggtcctcta gaccaaatcc
      541 ggctgaactg ggttcaaacc catctctctt gggtgcacgc ttggtctgga caagcaatct
      601 ggggagcgtt gacttcccgc tggccatcaa ggccaatcgt agtggtgtga tgcttgccag
      661 cgctgacggg tccgtggttt ccctcgatgc ccgcaccggt gcggcgggtt ggcgggcgaa
      721 tgttggaagc ccaatcgctg caggggtggg cagtgatggt cgctatgtcg ctgtagtgac
      781 gcgtgacagt gagttggtcg tgcttgaagc aggacgggaa ctgtggcgga gccgtctggc
      841 agctcaagta ttcacgtcac cattggttgc cggtgagcga gtgtttgtgt tgggcgcgga
      901 tcgaatgttg acaacgtttg atgcgcaatc agggcgtaag ttgtggcaat accagcgccc
      961 gggcgaagca ttggtgctac gccaggcggg tgtgctgtta cctgtacaca acaccttggt
     1021 cgttggtttt tctggtcgtc tccttggttt gaacccactt accggctctg tactatggga
     1081 tgcagccatc gctacgccgc gcggcaccaa tgacattgaa agactggttg atttggtggc
     1141 aggtgccagc cgtgaagccg atgtcgtatg tgtgcgtgca tttcaggctg ccgtggggtg
     1201 tgtcgagacg gttcgcggca aggtgatttg gcgccaagct gcatcgggat cagtgggcgt
     1261 gcacggtgat gaccaccagg tttacggtgt tgaagccgat ggtcgcgttt tgtcctggag
     1321 cgctagcgac ggtaagcagg tgtgggaaac ggatagattg cgttttcgcc agttgagtgc
     1381 gccgctggtg cttggcagat cagttgttgt cggtgataac tcaggctttg tgcattttgt
     1441 ctcccgtgtc gacggctcgt tactgacccg actcactacc gacggttcag ccatttccgt
     1501 caccccggtt tcggctgatg gaacgctggt ggtcgttacg cgcaacggtg gcgtgtttgg
     1561 ttttcggcca gagtgaagca acaccagttg cacctcgact gaaacaaatg aaacccgtca
     1621 tcgccttggt cggccgcccc aatgttggta aatcgacgct atttaaccga ttgacgaagt
     1681 ctcgtgatgc catcgtggca gactttttcg gactgactcg tgatcgacat tacggtaacg
     1741 gcaagcaagg caaacacgag ttcattgtca ttgacaccgg ggggtttgag cccgatgctg
     1801 caagtggcat ttacatggaa atggccaaac agacccgtca ggcggttgct gaggccgatg
     1861 tggtggtttt tgtggtggac gcgcgcggtg gtgtctcggc gcaagaccat gacattgcga
     1921 actacttgcg tcggctcggc aagccttgta ttttggtcgc caacaaggcg gaaggcatgc
     1981 ttgaaggcat tcaattggtt gagttttacg agttaggcct gggcgaagta catcctgttt
     2041 cagcggcgca tggacaaggc attcgctcct tgatagatgt tgcgctggaa ccctttggcg
     2101 cgctcaatga tgatgtaagc cccgaatttg aaccaggcgt gatcaagctg gctgttgccg
     2161 ggcgtcccaa cgtgggcaaa tccacgctga tcaatgtctg gctgggcgag gagagacttg
     2221 ttgcttttga tctgcccggc acaacccgag atgccatttc tgttcctttt gaacgcaatg
     2281 gccagaaatt tgaattgatt gacacggccg gtctgcgtcg gcggggcaag gtttttgagg
     2341 cgatcgaaaa attttcggtg gtaaagaccc tgcaggcgat tgagtctgcc catgtggttc
     2401 tgctgctgat tgatgcgact cagggtgtaa ctgaccaaga tgcgcacatt gctggctaca
     2461 ttcttgaaaa tggccgggcc gtagtgttgg cgatcaacaa atgggatgcc gtggatgagt
     2521 accaacgcga acttgtcaag cgatccattg aaaccaggtt agggtttttg aagttcgcca
     2581 atcttcattt gatttccgcg caaaaacgcc aggggcttgg gccattgtgg gtatccatag
     2641 ctcaggcctt caaagctgct acctgcaaga tgtccacgcc ggtattgaca aggttactgt
     2701 tggaatctgt gcaattccag agcccaaagc gcgcgggcat gttccgtccc aagttgcgtt
     2761 acgctcatca aggtggcatg aacccaccgg tgatcgtaat ccacggaaac tcactcgagc
     2821 atgtgaccga tgcttacaag cgttttctgg aaggacgttt tcgcaaggaa tttgacttgg
     2881 ccgggacacc tttgcgcatt gaaatgaaga catcaagtaa cccattttca gaaaaagctg
     2941 attgacctcg ggcatgtacc tttcaggtca aattaattgg gtgtttatga cggcttccaa
     3001 taaaaggcca gatgtctgtg gtatcgtgcc ctcttcttaa attatccgaa cacggagaat
     3061 atcgtgagca acaaaggcca actcttacaa gatccgtttc tcaacacact acgtcgggaa
     3121 cacgtgccag tgtctattta cctggtcaac gggatcaagc ttcaaggtca gattgagtca
     3181 tttgatcagt atgtcgtttt gctacgcaac acggtgacgc aaatggtcta caaacacgcg
     3241 atttcgacta tcgtgccggg tcgcgcagtc aatttctcag tggttgaggg tgacgaacct
     3301 ggctcagcct gatatcagaa gcgcccccac cttgttggtg ggtgttgatt ttggactgcc
     3361 ccggtttgac gaccaactgg aagaactggg tctgctagcc cagactgcgg ggctacaccc
     3421 agtggcgaga gtaacttgca agcggcgcgc gccagatgct gcgctgtata ttggccgtgg
     3481 aaaagctgac gaaatcaaac aactggcgct acaaatgggt gtccaggaaa tcttgtttga
     3541 tcaaagtttg agtccggcgc agcaacgcaa tcttgagcgg catcttgagt tgccagtgaa
     3601 tgatcgaacc ttgttgattc tgcaaatatt cgggcagcgt gcgcgcagtc atgagggtaa
     3661 attgcaggtt gagttggcgc gactacagta cctgagcacg cgcctggtgc gccgctggtc
     3721 tcacctggag cgccaaagcg gaggcatcgg catgcgcggc gggccgggcg aaacccaaat
     3781 cgaacttgat cgacggatga tcagcgacag catcaaacgc accaaggagc ggctggtcaa
     3841 ggtcaaacgg cagcggcaga cccaacgacg ccaacgtgag cggcgtgaaa ctttcaatat
     3901 ttcattggtt ggctacacca acgctggcaa atccaccctg ttcaatgccc ttgttaaagc
     3961 gcgtgcctat gcggccgatc agttgtttgc aacactagac accaccacgc gccagttcta
     4021 tttgggcgag attgggcgca tgatctcgct gtccgatacg gttggcttca ttcgtgattt
     4081 gccgcatggt ttgatcgatg cgtttcaagc caccttgcaa gaagcggtag acgcagatct
     4141 gctcatgcac gtggttgatt tttccagccc caatttcacc gagcaaattg agcaggtgca
     4201 acgggttttg gctgaaatcg gtgctgcaga tgtccctcag gtgctggtgt tcaacaaact
     4261 cgataggctc gaaccagaca accggccagc cgtgctgcaa gatgtttacg aactcgatgg
     4321 tctgccgacc ccgcgtgttt ttttgagcgc acgcgacggc gagggtttgg tggcgctgcg
     4381 tcgcttgctg tcggaaaggt tgcttccagt gcaaaacttg gtggtaccgg cagatttccc
     4441 tttgggccgt gaggctggcg tttgattcgg cacaatcggc gttcattaga caagagttga
     4501 ttcgatgaaa cttcaattac cgactttcag tttggcattc tggccgcagc gacttaaggg
     4561 aatgttcaac ctcaatgatc cccgttgggg acgctcggac gacaagcctg ctgacaacgc
     4621 cgatagcggt gagactgatc gtcctgcgac cggtgttcca gaaaccccac cgcgagccaa
     4681 cgaagatcgc aggccgcggc agggcggcaa tcaggggccg cctgatctcg atgaattgtg
     4741 gcgggatttc aatcgcaaac taggcggctt atttggtaac accaaagttg gcgggcgcgg
     4801 cggtcctgcc agtggcggca ccggtggggg caacaggaca cccggaggct ttcagcccga
     4861 tatgaaaaac gcaggtgttg gagctgggct ggtggtcgct gtagcggtgc tgatttggct
     4921 gggtacaggt tttttcattg ttcaggaagg ccagcaggcc gttattaccc agttcggtaa
     4981 gttcaactca accgtgggtg cgggcttcaa ttggcgtttg ccctatccaa ttcagcgtca
     5041 tgaagtcatt tttgtgacac agatcaggtc agttgatgtc gggcgcgata ctgtcatcaa
     5101 agccacaggc ttgcgagaat cggccatgct gacagaagac gaaaacatcg tcgaaatcaa
     5161 atttgccgtg cagtaccgct tgagcgacgc acgtgcattt ctttttgaga gcaaaaatcc
     5221 ggccgatgct gtggttcagg cagccgaaac ggctgttcgc gaggtggtcg gaaagatgcg
     5281 gatggacagt gctttgtcgg acgaacgaga ccagattgcg ccgcgggtga gagcattgat
     5341 gcaaaagatt ctggaccgct acaaagtcgg catcgaagtg gttggcatca acttgcagca
     5401 aggtggtgtg cgcccccccg agcaggtgca agccgcattt gatgatgtgc tcaaggccgg
     5461 gcaagagcgg gaacgagcca aaaacgaagc gcaagcctat gctaacgacg tgattccgcg
     5521 tgcggttggt gctgcatcgc ggctcaagga agaggccgat gcctacaagg cccgtatcgt
     5581 ggcacaggca cagggcgatt cccagcgctt caagtcagtg ctggctgaat accagaaggc
     5641 cccgcaagtc actcgtgatc gcatgtacat cgattccatg cagcagattt acagcagtgt
     5701 taccaagatc atgattgact cgcggcaggg ctccaatctc ttgtatttgc cattggacaa
     5761 gatcatccag atgacggggc aaacgtcgga cgacgcagtg cctgcaccgc tggcggttcc
     5821 tcccgcaggc gccccagcca acgcagccgc cacacctgtg gatgcgcgca cccgcgatgc
     5881 ggcgcgtgcc cgcacccgtg aatcccgcta ggagccccgc atgaatcggc taggttttgt
     5941 tatttccagt gttctttttg tgttggcaat tgccagttct accctgtttg tggtggatca
     6001 acggcagttt ggtgttgttt ttgctctggg gcagatcaag gatgtcatca ccgagcctgg
     6061 cttgaacttc aagctgccac cgccttttca gaatgtgtcc tacattgaca aacgtttgct
     6121 gacgctcgac agcactgaca acgaaccggt gttgacagcc gaaaagcagc gtgtggtgat
     6181 tgactggtat gttcgctggc ggatttccga gcccacccag tacatccgca acgtgggtac
     6241 caccgaggca gccggtgcca gccagttgaa ccgcgtggta cgcaacgcgt tccaggaaga
     6301 aatcaacaag cgcacggtca aggaactgct atcggcgaaa cgtgaggctt tgatggaaga
     6361 tgtcaaaggt gaggtacttg ggcaggtgcg tggtgccaag ccctggggtg tggatgtgat
     6421 tgatgtgcgc atcaccaggg tggactatgt tgatgcgatt accgagtcag tttatcgccg
     6481 gatggaagcc gagcgcaaac gggtggctaa cgaactgcgc tcgacgggtg ctgccgaggg
     6541 cgaaaagatt cgcgcagatg ctgatcgtca gcgtgaggtg acaatagcca acgcttaccg
     6601 tgatgcgcaa aagatcaagg gcgagggcga tgccgaggcg gcccgcacct atgccgaagc
     6661 gtttggccga gatccccagt ttgcccagtt ttatcgaagc ctgggtgctt accagtcaac
     6721 tttttccaag aagagcgacg tgatggtgct cgatccgtca agttccgagt ttttcaaagc
     6781 cctgcgcggc ggtgcggcgg gtgcgtcggt tgccccggcg caaggcaaga agtagccatg
     6841 gagacagggc tggacagcac cactttctgg cttgccgttg ccctggtgtt ggtgatcgag
     6901 ggctttatgc cttttctctc gccagccacc tggcgcaaga cctttcttca ggttctgcaa
     6961 cttggcgatg gtcagttgcg cttttttggc ttgtgcagcc tgttattggg tgttgcgctg
     7021 atttggtggc tggcctgacg gaatcagccg ctggcaaacg gtaaaatcgc gtttttaaca
     7081 gctcctaaaa atcacatgtc tgcttgggtc ctgccggatc acattgccga tgtcttgccg
     7141 tcagacgcac ggcacatcga agaattacgc cgagatctgc tcgatacagc gcgctgttat
     7201 ggctatgaac tggtcatgcc accgttgctg gagcatattc aatccttgct caccggtgct
     7261 ggcgttgcgc tggatttgca gacgttcaag ctggttgacc aactctcggg ccgcatgatg
     7321 gggctgcgcg ccgacagcac accccaggtt gcccgcattg atgcccatct gctcaatcgc
     7381 agtggtgtga cacgcttgtg ctactgtggg ccggtcctgc atacccgtcc cagtgccccg
     7441 catgccaccc gcgagccgct gcaattcggt gccgaaattt acggccacgc cggtctcgaa
     7501 gcagatatcg aggtgctgac cctggcgctg gattgcctga aggctgccaa ggtgcaggcc
     7561 ccgagtattg acctggcgga tgcccgtctt gtcaaggcga tgctctccgg catgtccgtc
     7621 aatgctgccg agctcgccaa cctgtacgcg gcactggccg ccaaggacag cccggaactg
     7681 atcaccttga cgcaacacat gcccgctgaa gtgcgtcagg gcctgttggc gctggtgcaa
     7741 ctgtatggcg acgatcatgt cctgattgag gctgaaaagg tgctccagcc cttaccggct
     7801 gtgcgtgaag cgctactcag tttgaagcaa ttggcaaacc atttgcaagg tgtgcaagtg
     7861 acttttgatc tggccgactt gcgtggttac gcctattaca ccggtgcacg ctttgccatc
     7921 tacgccccag cgggcagcga tgcgctggtg cgcggtggac gctatgacga ggtgggagcg
     7981 gtgtttggcc gcaaccgtcc ggcagcgggt ttcagcctgg atctgaaggc cttggtggcg
     8041 gctgtgcagc cacgcccgtt gcaggtcgcc attcgggcac cttggcgtga cgacatcgca
     8101 ctggccgatg ccatcgccgc tttgcgcaac cgcggtgaaa ttgtcatgcg cgtgttgcct
     8161 ggccacgaga gcgaagttga tgaattccat tgcgaccgca agctgattga agtggccggg
     8221 cagtgggttg tgcaagcgat ctgattgata agcgaaacaa aatgactgcg atcaaagggc
     8281 gtaatgtggt tgtcgttggc acccagtggg gcgacgaggg caaaggcaag ttggtcgatt
     8341 ggctgaccga gagtgcacag ggcgtggtgc gctttcaagg cggccacaat gccgggcaca
     8401 caattgtcat caacggtgtg aaaaccgcct tgcacctgat tcccagtggc atcatgcgtc
     8461 ccggtgtcaa atgctacatt ggcaacggtg tcgtgctgtc agcggccaag ctgtttgaag
     8521 aaatcgccgg cttggaaaaa gtcggggttg agttgcgttc acggctgcgc atcagcgagg
     8581 catgcccgtt gatcctgccg tttcacgccg cactggatgt ggcccgtgaa gcggcacgcg
     8641 agcagggcgg cattgaaaag attggcacca ccggccgggg catcggcccg gcttacgagg
     8701 acaagatcgc tcggcgcgcc ttgcgcgtgc aagacctgaa gttccctgag cgctttgcca
     8761 gcaagttgca cgatctgctg gacctgcaca accatgtgct gaccacctac ctgggttccc
     8821 gggcctttga ctttggcccg atgctggcac cttacatgaa agatggcaca gtgcagtttc
     8881 agccggtgta tgacgaggca atgcgtcatg ccgaactgct caagcccatg atggctgacg
     8941 tgtcacgcga actaaacaat gcccacctca aaggtgagaa ccttctgttc gaaggcgcgc
     9001 aagggacact gcttgacgtg gaccatggca cttacccgtt tgtcacctcc agcaactgtg
     9061 tggcaggtaa cgcggcggcc ggtgccggtg ttggcccggg cctgctgcac tacattctgg
     9121 gcatcaccaa ggcctattgc acgcgcgtcg gtggtggtcc gtttccgacc gagctggact
     9181 gggaacggcc tggcaccccc ggctaccacc tgagcacagt cggtgccgag aaaggtgtga
     9241 caaccgggcg cagtcgtcgc tgcggttggt ttgacgcagc attgctcaag cgctcggctc
     9301 aggtcaatgg cctgaccggg ctgtgcatca ccaagctgga cgtgctcgac ggcctgaaag
     9361 agttgctgct gtgcaccggt tatgaacttg acggcgacct caccgacatt ctgccgatgg
     9421 gagcggacga catcgcccgt tgcaaaccag tctatgaaac attggcgggc tggagcgaca
     9481 gcaccgtggg tgtcaccgaa tatgacaagt tgcccagcgc tgcccgtctg tatctgcagc
     9541 gcatcgagca ggttaccggc gtgccgatcc acatggtgtc caccagcccg gatcgtgacc
     9601 acaccatcct gatgcggcac ccctatcttg ccgactgatt gggtgctacc cggccccctt
     9661 gtcggtgata gataaaaagt gcctccagcc ctttaccaca ttgcgtagac agctattaat
     9721 tggggagtat ttcgatgttg acagaagacg gcaagcacct ttatgtaagc tatgacgaat
     9781 acaacaatct gattgaaaaa ctggctatca aggtatttca gtcgggctgg cagttcgaca
     9841 cgattttgtg tctggcgcgt ggcggcatgc gtccgggcga tgtcttgtcg cgggtgtttg
     9901 acaagccgct ggccatcatg tcaaccagtt cttaccgaag cgatgcggga acggttcagg
     9961 gcaagctcga cattgcccac tacatcacaa cccccaaggg cgaaatagcc ggccgggtgc
    10021 tgctggtgga cgacctggca gactctggtt tgacgctgaa tgcggtgatt cagcaactta
    10081 agaacaacta caagcccatc gaggagttgc gcagcgcggt tatctggacc aaggccttgt
    10141 ctaccttcac gccagactac tgtgtggact atttgccgac caacccgtgg attcaccagc
    10201 cttttgagaa ctacgacagc atgcgaccgg agcagctcat tcaaaaatgg agtgtctgat
    10261 tgtcttctga ccatgccaaa gcgatcagta ccgcgctgat ccaccattcc tatacgccac
    10321 cggctggttt cgctgcgccg caaagcgctg tgttcaaggc ctccacggtg tttttcccca
    10381 atgtagccgc gatgcggcgt tttgaatgga aagacaagac agcctacacc tatgggttgc
    10441 acggcacacc caccacctac acgctggaag agcggttgtg caccttggaa ggcggtcgtc
    10501 agtgcctgct gctgccgagc ggtctggcgg ccattgctgc ggtgtcgctg gcgctgctca
    10561 agtcgggtga cgaagtgttg ttgcccgaca atgtctacag ccccagcaag gccctggcgc
    10621 aaggcgaatt ggccaactgg ggcatttcgc accggtttta tgatgccatg cggccgcacg
    10681 acctggcggc caagattggt cccaacaccc ggctggtgtg gcttgaagcg gcgggctcgg
    10741 tcacgctgga gtttcctgat ctgtgcgaac aggtacgcat ttgcaaggcc gctggtgtgc
    10801 tgtgtgcgct ggacaacacc tggggagcgg gcctggcctt caagccgttt gatttacacc
    10861 cggacaaccg gtcagcactt gccgttgata tttccattca tgccctgacc aagtacccca
    10921 gcggcggtgg cgatgtgctg atgggcagtg tcatcaccgt ggaccaggcc ctgcacatga
    10981 agcttaagct gtgccacatg cgcctgggcc agggcgtggg tgccaacgat gccgaagccg
    11041 tgctgcgggg tttgcccagt ctggcactgc gctaccaggc tcacgatgaa gcgacacgcg
    11101 ccctggcgca gtggagcctg acacgcagcg agtttgccca ggtgctgcac ccggcgctgc
    11161 cccagtcacc gggtcatggc cagtggagca ggc
//
LOCUS       DAOSLL010000070        11102 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5808_length_11102_cov_4.442926, whole genome shotgun sequence.
ACCESSION   DAOSLL010000070 DAOSLL010000000
VERSION     DAOSLL010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11102
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5808_length_11102_cov_4.442926"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /gene="bamA"
                     /locus_tag="PLB25_04655"
     CDS             <1..1391
                     /gene="bamA"
                     /locus_tag="PLB25_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOE40908.1"
                     /translation="DEVARTTKAFTDFFGVFGYAFARVEAIPEVDRVNNRVSLTLQAN
                     PARRASVRKINVAGNTRTRDVVVRREFRQLEASWYDGDKIRLSRDRVDRLGYFGEVAV
                     ETHEVPGSPDQVDLTLNVTEKPTGSLTLGAGFSSADGLGLSFGLKQENAFGSGNSLGV
                     QVNTSKINRVLVLNTTDPYFTADGVSRTIDLYQKSSSPYENQDYYKLVTSGAAMRFGI
                     PFTEIDTVFFGAGVEQTNIKLGTLLPTSYQDYINEYGYKSSAVPVTVGWARDSRDSAL
                     SPTSGRLQRANGEWSIAGDARYVRGTYQFQQYLPLSKQYTAAFNAEVGLGRATGGQTF
                     PVFKNFYGGGLGSVRGFEQGSLGPRDISGTAVGGTRKVNLNFEVLTPFPGAGNDRSLR
                     MYGFLDAGMVVGNDGGLAINADADRLRASLGIGVSWISPVGPLRLALAKPIRKYDNDR
                     IQTLQFQIGTSF"
     gene            1424..1903
                     /locus_tag="PLB25_04660"
     CDS             1424..1903
                     /locus_tag="PLB25_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOE40909.1"
                     /translation="MVSSLCLLTVQAQELKLGFVSTDRVLKEASTAKAAQNKLEQEFS
                     KREKDLTEQGANIKSMADKFEREAPTLPETQRLARQKQLLDQDRDFQRKRREFQEDLN
                     ARKNEELQQVIQRANKVIKDIAIAEKYDFIFQDAVYVNPKNDVTDKVIKALNGSSGK"
     gene            2117..3106
                     /gene="lpxD"
                     /locus_tag="PLB25_04665"
     CDS             2117..3106
                     /gene="lpxD"
                     /locus_tag="PLB25_04665"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HOE40910.1"
                     /translation="MALRLGSIVESLGGALHGNADLLIEGLAPLQSATRHQLSFLSHP
                     QYQNQLADTLAGCVIVTAQFEQVAAARGACIVTDQPYLYYARVTQLWKKSLPTFAAAH
                     IHPSAVIDPLATVHPTARIGALCVVERGASIGADTELKSRVTVSEGCTVGQRCLLHPG
                     VVIGADGFGFAPNAGAWEKIEQLGAVLIGNDVEIGANTCIDRGALQDTVIEDGVKLDN
                     LIQIGHNVRVGCHTAMAGCVGVAGSASIGAHCTVGGGAIVLGHLTLAEGVNISAASVA
                     TRSIHKPGNYTGMFPIDDNAAWEKNAASLRQLASLRERIRTLEAQVKTLQNTI"
     gene            3119..3556
                     /gene="fabZ"
                     /locus_tag="PLB25_04670"
     CDS             3119..3556
                     /gene="fabZ"
                     /locus_tag="PLB25_04670"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HOE40911.1"
                     /translation="MDIHKILKQLPHRYPFLLVDRVLSIDKGKTIRALKNVTINEPFF
                     EGHFPNRPVMPGVLMLEAMAQAAALLAFDAMDATPLDDMVYYFAGIDGARFRRPVEPG
                     DQLILEADLLRIRAGIFKFKGRALVDGELVAEAELTCAIRKFA"
     gene            3567..4361
                     /gene="lpxA"
                     /locus_tag="PLB25_04675"
     CDS             3567..4361
                     /gene="lpxA"
                     /locus_tag="PLB25_04675"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HOE40912.1"
                     /translation="MTAIHATAIVDPGAEIDSSVTIGPYCVIGPHVKIAAGSTVGPHV
                     VIEGHTTIGRDNRIFQFVSLGAIPQDKKYAGEPCELVIGERNTIREFCTFNIGSPGDA
                     GVTRLGNDNWIMAYVHLAHDCQVGNNTIFANNATLAGHVQVGDWAILGGFTGAHQFVR
                     IGAHSMTAISTVLLADVPPFVMCQGQPGAARSMNFEGLRRRGFSPERMAAVKGMYKAL
                     YRDNLTLQVACEHIAQLDKSDPQCTPDVQMMLSFLQQVSPQRGIVR"
     gene            4543..5691
                     /gene="lpxB"
                     /locus_tag="PLB25_04680"
     CDS             4543..5691
                     /gene="lpxB"
                     /locus_tag="PLB25_04680"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HOE40913.1"
                     /translation="MLTAALVAGETSGDLLAGLLLDGLKARWPDLRCSGIGGPQMARR
                     GFDAWWPHDKLSVHGFGWEVLRRYREILGIRNQLKKRLLAQPPDVFIGVDAPDFNLDL
                     EACLRRQGIRTVHFVCPSVWAWRPERLEKIRRSADQVLCIFPFEPALLAQHGIAATYV
                     GHPLANVIPMQSDRKRARAALGLSPHAQVVAILPGSRESEVRHLALRFFQAAALISQA
                     LPAIEFIVPAIPLLIRQIEAAAMASGLGARLRIVPGQSHSVLAACDVTLIASGTATLE
                     AALFKRPMVIAYHMGALSWQIMRRKRLQAWVGLPNILCREFVVPELLQDAATPPALAQ
                     EVLSWLNAPRLQPQRISALEQRFATLHAQLQRDTPALAAHAIAQMLSS"
     gene            5765..6370
                     /gene="rnhB"
                     /locus_tag="PLB25_04685"
     CDS             5765..6370
                     /gene="rnhB"
                     /locus_tag="PLB25_04685"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HOE40914.1"
                     /translation="MLQLARQWGVNGLVAGVDEAGRGPLAGPVVAAAVILDDLDPVSG
                     LADSKQLTALKRERLFDEIRAKALCFSVAQASVAEIDAINILQATLLAMRRAVEGLRL
                     TPKLVLVDGNRRPLLPMRCEAIVKGDALVAAISAASILAKVTRDRWCVSYDQQFPQYG
                     FARHKGYGTLVHLAALQAHGACPEHRKTFRPVSLALAGSKS"
     gene            6367..7179
                     /locus_tag="PLB25_04690"
     CDS             6367..7179
                     /locus_tag="PLB25_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOE40915.1"
                     /translation="MSAPSVTHVSSRDNPVFKALRSLAHGNTGYRKQGRIWAEGEHLS
                     LAALNRGMRPIMGVFSESFWPLAQALYAQSATVNIVLQDALFDAVSSLASPARMGFVF
                     ELPPPPVIVSQAPSVILDRLQDPGNVGSVLRSASAFGFGQVIALQGTAALWSPKVLRA
                     GMGAHWGLQLIEGVGPAELGQLTLPILVTSSHQGDYLHQMIQKNELPRHCAWAFGHEG
                     QGVCAEIEALASLHVRIAQPGGEESLNVAAAAAICLHASATACSGRIQPQAS"
     gene            7355..10072
                     /locus_tag="PLB25_04695"
     CDS             7355..10072
                     /locus_tag="PLB25_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HOE40916.1"
                     /translation="MTLAQLPVEAALASLKSQAGGLTTDEADRRHLEFGFNRVETVAR
                     EPLWLSFGREFSHFFALILWVAAGLAFFAESREPGQGMAQLGITIVCVILINGIFSFW
                     QTYRAEQALDALRRLLPQQVSVLRDASVQTLAAELLVPGDVLLLAEGDRVPADCRLIE
                     AFDLRVNLSTITGESQPKARNAAPDPELSPLDARNLLLAGTLVVAGECRAVVFATGRH
                     SEFGKIAHLTQSIGATSSPLQREIARLSHLVALSASTLGVVFFFIGQAMGLPFWSNLL
                     FAIGIIVANVPEGLLPTVTLSLAMATQRMARRNALVRHLSAVETLGSTTVICSDKTGT
                     LTQNRMSVIKVFAEDDFHQPGASLPKALLQVAGNCHTLRFADGRPMGDPMEIALWELA
                     ANQSQAEARRVWSAEIAFDTGRKRMSVINGAGVDRVLYCKGAPEVVLPLCSSVLLGGQ
                     AVALNAATQARWVTAQDAMMDQGLRVLMLAYKPLASDEDPSEQEMVLCGLVGLQDPAR
                     AEVPAAIARCHTAGVKVIMVTGDHPHTALAIAREIGLVRGASPEVLIGAMLPKMSASQ
                     LQLALEAPEIIFARVTAEQKMLIVQALQRKGEIVAVTGDGVNDAPALKVADIGIAMGL
                     SGTDVAKEAADIILLDDNFASIVSAIEEGRAVFENLRKFLTYILSSNVPELVPYLAFM
                     LLRIPLPLTVIQILAVDLGTDMLPALALGAEAPHPGLMQQPPRARSERLLSWGMVLRA
                     YLWLGVLEAGIAMAAYYFVLNASGWRYGDLLPVGDPAYLRATTACFAGIVVAQMVNVF
                     VCRHPQQSSLRFGLFGNPLLLAGLALELVLMLLIIYTPWGNTLFGTAALPIEVWLLVL
                     PLALLMGLLEEGRKAWLRRQLARKAGSPQGDESRLGAKASAPPSAPDTP"
     gene            complement(10465..>11102)
                     /locus_tag="PLB25_04700"
     CDS             complement(10465..>11102)
                     /locus_tag="PLB25_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE40917.1"
                     /translation="GKSTLFDLISGRLAPSSGQVWLGGQRIDGKPAFEINRLGLSRSF
                     QISSLFANLSVFDNLRCGLLWQQGLRYTLFKRLSRLHQANERTEQLMEQVQLSSRRTT
                     LAANLSYAEQRALELGITLASGAGVILLDEPTAGMSHTEATAFVQLIRSVTVAKTLLI
                     IEHDMPVVFGLADKIAVLAQGELLAFDAPDAVRADARVQAAYLGAGSSSMV"
BASE COUNT         2088 a         3297 c         3432 g         2285 t
ORIGIN      
        1 ccgatgaggt ggccaggacg accaaggcgt tcactgattt ctttggcgtc tttggctacg
       61 cctttgcccg tgttgaagcg atccctgaag tggaccgggt gaacaaccgg gtgtcgttga
      121 cgctgcaggc caacccggcg cgtcgcgcct ctgtccgcaa gatcaacgtc gcgggcaata
      181 cccgtacacg cgacgtggtg gttcggcgtg agttccgtca acttgaagcg tcttggtacg
      241 acggtgacaa gatcaggctt tcgcgcgacc gggttgaccg actgggttat tttggtgagg
      301 tggccgttga aacccacgaa gtgccaggtt cgcccgacca ggttgatttg acacttaacg
      361 tcaccgaaaa gccgaccggg agcctgacgc ttggtgccgg tttctccagt gctgacggct
      421 tgggcctgtc atttggcctg aagcaggaaa atgcctttgg ttccggcaat tctctcggtg
      481 ttcaggtcaa taccagcaag atcaaccggg tactggtgct caacaccacc gacccctatt
      541 tcacggcgga tggtgtttcg cgaaccattg acctctatca gaagtccagc agcccgtatg
      601 aaaatcagga ctattacaag ctggtcacct cgggggccgc gatgcgtttt ggcattccgt
      661 ttaccgagat cgacaccgtg ttttttggtg ccggggttga gcaaaccaat atcaagctcg
      721 gcacactgtt gccgacctcg taccaggact acatcaacga atacggttac aaaagctcgg
      781 ccgtacctgt gacggtcggc tgggcgcgcg acagccgtga cagcgctctc tcgcccacca
      841 gtggaaggtt gcagcgtgcc aatggcgagt ggtctattgc cggagatgcc cggtacgttc
      901 ggggtaccta ccagttccag cagtacctcc ctttgagcaa acaatacacc gctgcgttca
      961 acgctgaggt cggcctcggc cgggcaacag gtggccagac cttccctgtt ttcaagaatt
     1021 tttatggcgg cggcttgggc tcggtacgtg gcttcgagca gggcagtctg ggtccaaggg
     1081 acatcagcgg cacggctgtg ggaggcaccc gcaaggtcaa tttgaacttt gaggtgttga
     1141 ccccgtttcc gggtgccggc aatgaccgca gtcttcggat gtatggcttc ctcgacgcag
     1201 gcatggtggt tggaaacgac ggcgggctgg cgatcaatgc cgatgctgac cgcctgcgtg
     1261 catcgctcgg cattggtgtg agctggatat ctccggtggg cccattgcgc ctggcattgg
     1321 ccaagccgat tcgcaagtat gacaacgata gaatccaaac cctgcaattt caaatcggaa
     1381 ccagtttcta atgaatcaaa atttacgtcg tttggctgcc ttgatggtca gcagtttgtg
     1441 tctgttgaca gtacaggcgc aagagctcaa gcttggattt gtcagtaccg accgggtgct
     1501 caaggaagcc agcacggcca aggcagccca gaacaagctg gagcaggagt tttccaagcg
     1561 tgaaaaagat ctgaccgaac agggcgcaaa catcaaatcg atggctgaca aatttgagcg
     1621 cgaggcaccc accttgccag agacgcagcg tctggcgcgg caaaagcagt tgcttgacca
     1681 ggatcgcgat tttcaaagaa agcgccgcga gtttcaggaa gacctcaacg cccgcaaaaa
     1741 tgaagaactg caacaggtca tccagcgcgc caacaaggtc atcaaagata ttgccatcgc
     1801 cgaaaaatac gacttcattt ttcaggatgc ggtttatgtc aatcctaaaa atgacgtgac
     1861 cgacaaggtc atcaaagcgc tcaacggatc ttctggcaag taaggacatg gtcatccata
     1921 acttccccat ttggcgcgtc gggttggggc gcactgcgtc gctgcgcgtc gctggtgtgg
     1981 cttgccacat gtcaaacgga cccagtcagg tcggtttgag gtcgcgcttc gcgcctggca
     2041 ttgcatgcca agtccaaggg ccaaatgcgt aacttgtttt tgactctatc ctgagcaagg
     2101 tcgaggcttg aggtcagtgg cgctgcgttt agggtccatt gttgaatcgc ttggcggcgc
     2161 gttgcacggt aacgccgacc tgctgatcga aggtttggca ccgctgcaaa gtgccaccag
     2221 gcatcaactc agctttctaa gccatcccca atatcaaaac cagttagccg acacactggc
     2281 aggctgtgtc attgtgactg cgcagtttga gcaggttgca gcggcgcgcg gggcctgcat
     2341 cgtcaccgat cagccctatc tgtattacgc acgcgtcacc cagctctgga aaaagagcct
     2401 gccgaccttt gcagcggcac atattcatcc ctcggcggtg attgatccgc tggcaacggt
     2461 acaccctacc gcccgaattg gagccctgtg cgtggtcgag cgtggtgcca gcattggtgc
     2521 cgacaccgag ttgaaatccc gcgtcaccgt cagcgagggt tgcacggttg gccagcgttg
     2581 cctgctgcac ccaggggtgg tgatcggtgc cgacggattt ggttttgcac ccaacgccgg
     2641 ggcctgggaa aaaattgaac aactcggtgc cgtgctgatt ggcaatgatg tcgagattgg
     2701 tgccaacacc tgtatcgacc ggggtgcctt gcaagacacg gtgattgaag atggtgtcaa
     2761 acttgacaac ctgatccaga ttggccacaa cgtgcgtgtt gggtgccaca ccgccatggc
     2821 aggctgcgtc ggtgtcgccg gtagcgccag cattggtgcg cattgcacgg tcggtggggg
     2881 cgctatcgta ctggggcact tgacgctggc cgaaggggtc aacatctccg ctgcatccgt
     2941 ggcgactcgc tcgattcaca aacctggcaa ctacaccggc atgtttccca tcgacgacaa
     3001 cgcagcgtgg gaaaaaaacg ctgcctcgct caggcaactg gccagccttc gcgagcgtat
     3061 tcggacactg gaagcgcaag tcaagacact tcaaaacaca atctaaccag tcaaccgcat
     3121 ggacattcac aaaattctga aacagttgcc acaccgctac ccatttttgc tggtggatcg
     3181 ggtgctgtcg attgacaagg gcaaaaccat ccgggctttg aagaatgtca ccatcaatga
     3241 gccgtttttc gagggccatt tccccaatcg cccggtgatg cccggcgtgt tgatgctgga
     3301 ggcgatggcg caagcggccg ccttgctggc gtttgacgcg atggacgcaa cgcctctgga
     3361 tgacatggtg tattactttg ccggcattga tggcgcgcgc tttcggcggc cggtcgagcc
     3421 gggtgaccag ttgatactgg aagcagacct gctgcgcata agggcgggta ttttcaagtt
     3481 caagggacgt gcactggtcg atggagaact ggtggccgaa gccgaactga cctgtgccat
     3541 tcgcaagttt gcctgaggag cccggtgtga ctgccatcca tgccactgcc attgttgatc
     3601 cgggtgccga gatcgacagc tccgtcacta tcggacccta ctgcgtcatc gggccgcacg
     3661 tcaaaattgc tgctggcagc acggttggcc cgcacgtggt gattgagggc cacaccacca
     3721 tcggtcgcga caaccggatt tttcagtttg tctcgctcgg ggccattccc caggacaaaa
     3781 aatacgcagg cgagccctgc gaactggtga ttggtgagcg caacacgatc cgcgagtttt
     3841 gcactttcaa catcggctcg cctggcgacg caggtgtgac acggcttggc aatgacaact
     3901 ggatcatggc ctatgtgcat ctggcacacg actgccaggt cggcaacaac accatttttg
     3961 ccaataacgc gacgctggcg ggtcacgtgc aggtcggcga ctgggcaatt ctgggtggtt
     4021 ttaccggcgc ccaccagttt gtcagaatcg gggcccacag catgacggcg atcagcacgg
     4081 tgctgcttgc cgacgtgccg ccgttcgtca tgtgtcaggg ccaacccggg gcagcgcgtt
     4141 cgatgaattt cgagggtttg cgccgacgcg gcttttcgcc cgaacgcatg gcagcggtga
     4201 agggcatgta caaggcactt taccgcgaca acctgaccct gcaggtggca tgtgagcaca
     4261 ttgcgcaatt ggataaaagt gacccgcagt gcacaccaga cgtgcaaatg atgctgtcgt
     4321 ttctgcagca agtgtcgccc cagcgcggca ttgttcgcta gccgcctgct gcaccatgga
     4381 ccaagcagcc agcagccacc ccagcacggg cagtgggtgc ctgatttgct ggcccattgc
     4441 ccggttgggg cccagattcc cggtaccaga taggccgctg ccgtcggttg gcagccgatc
     4501 tgccgcaggt ccggccgact cttagcgcag caggcttttc gcatgttgac tgccgcgctg
     4561 gtagccggtg aaacctccgg tgatttgttg gcgggtttgc tgcttgatgg actgaaggca
     4621 cgctggccag acctgcgttg ctcaggtatc ggcgggccgc aaatggcccg gcggggtttt
     4681 gatgcctggt ggccgcatga caaattgtcg gtgcacggct ttggctggga agtgctgcgc
     4741 cgctaccgcg agatcctcgg catccgcaac cagctcaaaa agcgcctgct ggcacagccg
     4801 cctgacgtgt tcattggtgt cgatgcaccg gatttcaacc tcgacctgga agcttgcctg
     4861 cgccgccagg gcatccggac ggtgcatttt gtctgcccct cggtgtgggc ctggcgacct
     4921 gagcgacttg agaaaatcag gcgcagcgct gatcaggtgc tgtgcatttt tccgtttgag
     4981 ccggcactgc tggcgcagca tggcattgcc gccacttacg tcgggcaccc gctggccaat
     5041 gtgattccga tgcagagtga ccgcaagcgg gctcgcgcgg cgctgggtct gtccccgcat
     5101 gcgcaggtgg tggccatatt gccaggcagc cgggaatccg aggttcgcca cctggctttg
     5161 aggttttttc aggctgcagc ccttatcagt caagcgctgc cagctattga attcatagtg
     5221 ccagcgatcc cactgctgat ccgccagatt gaggctgcgg cgatggccag cggactcggg
     5281 gcgcgcctgc gcatcgtgcc aggccagtcg cacagcgtgc tggccgcttg cgacgtgacc
     5341 ctgattgcca gtggcaccgc cacgctggag gcggcgctgt tcaagcgccc gatggttatc
     5401 gcttaccaca tgggtgcgtt gtcctggcaa atcatgcggc gcaaacggct gcaagcctgg
     5461 gtcggtttgc ctaacatcct gtgccgtgaa tttgtcgtgc ccgaactgct gcaggatgcc
     5521 gccacgccac cagcactggc acaggaagtg ttgagctggc tgaacgcgcc acgcttgcag
     5581 ccccagagaa taagcgctct cgaacaacgc tttgcaacct tgcacgccca attgcagcgt
     5641 gacacccctg cccttgccgc ccatgccatc gcgcaaatgc tttcaagctg aactggttga
     5701 aaccgattta cccccccaac ccaggggtcg gcgggtcagg cctgccaaaa ttttgcaggc
     5761 cagcatgttg cagctcgccc ggcaatgggg tgtcaatggc ttggtggccg gggtcgatga
     5821 ggccggacgc ggtccgcttg ccggtccggt ggtggctgct gcggtgattc tggatgatct
     5881 tgacccggtc agcggcctgg ccgactccaa gcaactcact gcgctaaagc gtgagcggct
     5941 gtttgatgaa attcgggcca aggctttgtg tttttcggtt gcccaggcca gcgtggccga
     6001 aatcgatgcc atcaacatct tgcaggccac tttattggcc atgcggcgcg cggtcgaggg
     6061 cttgcgtcta acccccaagc tggtgctggt cgatggcaac cgccgaccgc tgctgccaat
     6121 gcgctgtgaa gccattgtca agggtgacgc gctggtggcg gccatttcgg ctgcctccat
     6181 tctggccaag gtaacccggg accgctggtg cgtctcatac gaccagcaat ttccacaata
     6241 tggctttgcc aggcacaaag gctacggtac gctggtgcat ctggccgcct tgcaggcgca
     6301 tggggcttgc ccggagcacc gcaagacctt tcgcccggtc agccttgctt tggcgggcag
     6361 caagtcatga gtgcgccatc ggtcacgcac gtcagctcgc gtgacaaccc ggttttcaag
     6421 gccttgcgca gcttggcaca tggcaacacc ggctaccgca aacaggggcg catctgggcc
     6481 gaaggcgagc atttgagcct ggctgcgctg aaccgcggca tgcgtcccat catgggcgta
     6541 ttttcagagt ctttttggcc tctagcccaa gcgctatatg cgcagagcgc tactgtaaac
     6601 atagtgttgc aggatgcctt gtttgacgct gtcagcagcc tcgcctcacc cgcccggatg
     6661 ggttttgtat ttgagctgcc accgcccccg gtcattgtga gccaggcacc gagtgtgatt
     6721 ctggaccgct tgcaagaccc cggcaatgtt ggctcggtac tgcgcagtgc ctcggcattt
     6781 ggctttgggc aggtgatcgc gctgcagggc acggccgcct tgtggagccc caaagtgctg
     6841 cgcgctggca tgggcgcgca ctgggggctg caactcatcg aaggggtcgg cccggccgaa
     6901 cttgggcagt tgacgctgcc gatcctggtg acaagttcgc atcagggcga ctacctgcat
     6961 caaatgattc aaaaaaatga gctacccagg cattgcgcct gggcgtttgg gcatgaaggt
     7021 cagggggttt gcgccgaaat cgaagcactg gccagcttgc acgtgcgcat tgcccagccg
     7081 ggcggggagg aatcgctcaa cgtggcagcg gcggcggcca tttgcctgca cgccagcgcg
     7141 acggcgtgct ctggcaggat ccagccgcag gcttcctgag ccagccaggg ccagccctgt
     7201 gccaattgca gggatggcat tttgggcggg ctcgcgccag tgatcaagcg gtcgatggcg
     7261 caatacaggc cataaccacc gcggcaactc tgcccgaatg ttcagtgcac cagcccacca
     7321 tttggcgcat gctgcaggag cacaatgttg catcatgacg cttgcgcaac ttcctgtcga
     7381 ggcggcactg gccagcctca aatcccaagc cggtggcctg accactgacg aagctgatcg
     7441 ccgacacctg gagttcggct tcaaccgggt tgagacggtc gcgcgcgagc ccttgtggct
     7501 ttcctttggc cgcgaattca gccacttttt tgcgctgatc ctttgggtag ccgctggcct
     7561 ggcatttttt gccgaaagcc gggaacccgg ccagggcatg gcccaactgg gcattaccat
     7621 cgtctgcgtg atcctgatca atggcatctt ttctttctgg caaacctacc gtgctgaaca
     7681 ggcgctcgat gccttgcgca ggctgctacc gcagcaagtc agcgtgctgc gcgatgccag
     7741 cgtgcaaacg ctggcggccg aactgctggt gcccggcgac gtgctgttgc tggccgaggg
     7801 cgaccgggtg ccggctgact gccgcctgat cgaagcattt gatttgcgcg tcaatttatc
     7861 gaccatcacc ggtgagtcgc agcccaaggc gcgcaacgcg gcacctgacc ccgagctgtc
     7921 gccgctggac gcgcgcaacc tgctgctggc cggcacgctg gtggtggctg gcgaatgccg
     7981 ggcggtggtg tttgccaccg gcaggcattc cgagtttggc aagatcgccc acctgacgca
     8041 gtcgattggg gccaccagct cgccgctgca gcgcgagatt gcccgcttgt cgcatctggt
     8101 ggccttgtcg gccagcactt tgggggtggt ctttttcttc atcgggcagg ccatgggttt
     8161 gccgttctgg agcaatctgc tgtttgccat tggcatcatt gtggccaatg tgccagaggg
     8221 gctgctgccc accgtcaccc tgtcgctggc catggcgacc cagcgcatgg cccggcgcaa
     8281 cgcgctggtg cggcacctgt cggcggtcga aacgctgggc agcaccacgg tcatctgctc
     8341 ggacaagacc ggcaccttga cgcaaaatcg catgagcgtg ataaaggtgt ttgccgagga
     8401 tgactttcac cagcctgggg ccagcttgcc aaaggccttg ctgcaagtgg ctggcaattg
     8461 ccacacgctt cgctttgctg acgggcggcc gatgggcgac ccgatggaaa tcgcactgtg
     8521 ggagctggcg gcaaaccagt cgcaggccga ggcacgtcgt gtctggtcag ccgaaattgc
     8581 ctttgatacc gggcgcaaac gcatgtcggt gatcaacggg gctggtgtcg accgggtgct
     8641 gtactgcaag ggcgcgccag aagtggtgct gccgctgtgc agcagcgtgc tgcttggcgg
     8701 ccaggcggtg gcgctgaacg ctgccacgca ggcccgctgg gtcaccgcac aagatgccat
     8761 gatggaccag ggcctgcggg tgctgatgct ggcttacaaa ccactggcaa gcgacgaaga
     8821 cccaagcgaa caggagatgg tgctgtgcgg cctggtcggt ttgcaggacc cggcgcgagc
     8881 ggaggtgccc gctgccatag cgcgctgcca cacggcaggt gtcaaggtga tcatggtgac
     8941 cggcgatcat ccgcacacgg cgctggcgat tgcgcgcgaa atcggactgg tgcgcggggc
     9001 cagcccggag gtgctgattg gcgcgatgct gcccaagatg tcggcatcgc aattgcagct
     9061 tgcacttgaa gcaccagaga tcatcttcgc ccgcgtcacc gccgagcaaa agatgctgat
     9121 cgtgcaagcc ttgcagcgca agggcgagat cgtggcggtc accggagatg gcgtcaatga
     9181 tgcgccagcc ctcaaggtgg ccgacattgg cattgccatg ggcttgtctg gaaccgatgt
     9241 ggccaaggag gcggccgaca tcatcttgct ggacgacaac tttgccagca tcgtcagcgc
     9301 cattgaggaa gggcgggcgg tatttgaaaa cctgcgcaag ttcctgacct acatcctgtc
     9361 gtccaatgtg cccgaactgg tgccttatct ggctttcatg ctgttgcgca ttccgctgcc
     9421 gctgacggtg atccagatcc tggcggttga cctgggcacc gacatgctgc cagccctggc
     9481 gctgggtgcc gaggcaccgc accccggcct gatgcaacag cccccgcgcg cgcgcagcga
     9541 acgcctgttg tcctggggca tggtgctgcg ggcctatttg tggctgggtg tgctcgaagc
     9601 cggcatcgcc atggcggcct actattttgt gctcaacgcc agtggctggc gctacggcga
     9661 cctgttgccc gttggcgacc cggcttacct gcgcgccacc accgcctgtt ttgccggcat
     9721 cgtcgtggcg cagatggtca atgtgtttgt ctgccgccac ccgcagcaat cgtcgctgcg
     9781 ttttggcctg tttggcaacc cgctgctgct ggccgggttg gcgctggaac tggtgctgat
     9841 gctgttgatc atctacaccc cctggggcaa caccctgttt ggcaccgctg cgctaccgat
     9901 tgaagtctgg ctgctggtgc tgccgctggc gctgctgatg ggcttactgg aagaagggcg
     9961 caaagcctgg ctgcgccggc agctcgcccg caaggctggc agtccgcaag gggacgaaag
    10021 caggcttggg gccaaagcca gtgctccccc ttccgcgcca gacacgcctt gagcttgaag
    10081 cctggtgggc tgccggacgt atcctggact cctcagttga ccgcttgtat tcatcggtaa
    10141 gtcctcatga atgttgactt ttcagcgtcg atggggttca cccattggct gacagcgcta
    10201 cgcacccgat tgagccaccg gcaacacgcc tggggtcgtt acgccttctt gcgggcaaga
    10261 gctgtgctgc gtcgtcgccc ctgcggggcg cagccatgcc agaggcatat ggcctgcttc
    10321 aagccgtcca aacgtgcgca ggcacgtttg gagccgcggc tttcgcccta cccagacgcg
    10381 ccgccagcag cccattcggt cgcgtccaac tgagcaatcc aggcttacgg caacgatagc
    10441 gccaatcgac cccggcgaaa acagtcagac catggacgaa ctgccagcac ccagataggc
    10501 cgcttgcacg cgggcatcgg ctcgtaccgc gtccggcgca tcaaaagcca gcagctcgcc
    10561 ctgcgccagc accgcgattt tgtctgccag cccaaatacc acgggcatgt cgtgctctat
    10621 gatcaatagc gtcttggcaa ccgtcaccga cctgatcaac tgcacaaagg cggtcgcctc
    10681 ggtgtgactc atgccagcgg tgggctcgtc gagcaggatc accccggcac cactggccag
    10741 cgtgatgcca agctccagcg cgcgctgctc ggcatagctc agattggcgg ccagggtcgt
    10801 gcgtcggctg ctcaattgca cctgctccat gagttgttcg gtccgctcat ttgcctggtg
    10861 cagcctggac aagcgtttga acagggtgta ccgcaagcct tgctgccata gcaggccgca
    10921 gcgcaggttg tcaaacacgc tcaaattggc aaacaggctg ctgatctgga aactgcgtga
    10981 caaacccagc cggttgattt cgaacgccgg tttgccgtcg atgcgctgcc cacccagcca
    11041 aacctggccg ctgctgggcg cgaggcgacc gctgatcagg tcaaacagcg tcgatttgcc
    11101 gg
//
LOCUS       DAOSLL010000071        11077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5830_length_11077_cov_4.328888, whole genome shotgun sequence.
ACCESSION   DAOSLL010000071 DAOSLL010000000
VERSION     DAOSLL010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 11077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11077
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5830_length_11077_cov_4.328888"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            73..603
                     /gene="nusB"
                     /locus_tag="PLB25_04705"
     CDS             73..603
                     /gene="nusB"
                     /locus_tag="PLB25_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HOE40918.1"
                     /translation="MTESSHPAATRPPRQSRKGLTSTGVLKAASKSPRSRAREFALQA
                     LYQVLVGKNELSDVDAFTRDLAGFSKADVVHFDALLQGCGAQALALDALVQPLLDRPL
                     TEISPIEHAVMWIGAFEFQHCPDVPWRVVLNECIELAKEFGGTDGHKYVNAILNALAS
                     TLRPAEVAADRGASRA"
     gene            621..1805
                     /locus_tag="PLB25_04710"
     CDS             621..1805
                     /locus_tag="PLB25_04710"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOE40919.1"
                     /translation="MNISARAQRIEPFYVMEIAKAAQALAATVARSAQPMIFLNIGEP
                     DFTAPILVQEAAAKAIRDGQTQYTPATGLPELRERISQWYASRFQVAVPASRIVVTAG
                     ASAALQLACLALIEAGDEVLMPDPSYPCNRHFVAAADGTAVLIPTTAAQRFQLSAEQV
                     AQAWGDKTRGVLLASPSNPTGTSIAFDELRRIHQQVSQRGGITLVDEIYLGLSHDAQF
                     GRSALAIDDQIISINSFSKYFNMTGWRLGWLVLPLQLVPVIERLAQNLFICPSTISQY
                     AALACFEPDSLAEFEQRRAQFKARRDYFIPALNALGLSVPVMPDGAFYAWADCSAACS
                     KLKVNGSWDLAFELMKRAHIAITPGRDFGQAETAHFIRLSTANSMPQLREAVDRLQTL
                     LA"
     gene            1802..2281
                     /locus_tag="PLB25_04715"
     CDS             1802..2281
                     /locus_tag="PLB25_04715"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HOE40920.1"
                     /translation="MNSVASTQPAQAHKPFYWPVRIYWQDTDAGGIVHHANYLNFFER
                     ARTEWLRALGIGQQSLRETTGGMFVVSQANLSYVKSARLDDELLLTTSLTEAGRVAMT
                     IRQQALRKTLGANLLLCEANLRLAWVNADGKPARIPPVILSTLSQGIQPSPFVINQQ"
     gene            2278..2970
                     /gene="tolQ"
                     /locus_tag="PLB25_04720"
     CDS             2278..2970
                     /gene="tolQ"
                     /locus_tag="PLB25_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HOE40921.1"
                     /translation="MNQELSILHLVLNASIVVQAVMLLLISVSVASWAAIFRKLFSLG
                     KVKSLNDAFEREFWSGSSLNDLFSAATKNARNAGPMERIFASGMREFQKLRERRITDA
                     GTLMDGARRAMRASFQREMDVVETHLSFLASVGSVSPYVGLFGTVWGIMHAFTGLAAL
                     TQVTLATVAPGIAEALVATAIGLFAAIPAVVAYNRFARDIDRIAIRLETFIEEFSNIL
                     QRNVGAQSASGH"
     gene            2988..3407
                     /locus_tag="PLB25_04725"
     CDS             2988..3407
                     /locus_tag="PLB25_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOE40922.1"
                     /translation="MPSMTSRGRGRRTISEINMVPFIDVMLVLLIIFMVTAPLIAPSM
                     ISLPSVGKGDKQPDQVVQIIIGKDETLALKLKDQTSPVALKELVRSVRQAQGGVANSA
                     VVISADKSVKYEAVVRVMDTLQRAGVARVGLSVQLAK"
     gene            3417..4439
                     /gene="tolA"
                     /locus_tag="PLB25_04730"
     CDS             3417..4439
                     /gene="tolA"
                     /locus_tag="PLB25_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HOE40923.1"
                     /translation="MHAGADRLEFAPPATPGLLRAMVLALLAHGLLLIALTWGVHWQR
                     DVQVASYEAELWSALPTEAAPALVETPPPEPPEPPPPLPPVPEPLPVPKPVPAPPEPK
                     PVPAPKLPEVDIALQQEKQRLKKQQEQKQEEQRQDKLKAEKLKLEKIKQEKAELETAK
                     LNKLKQEKLKQEQLRQEQLRQEQLRQEQLKQEKLKQAQADDAQKAAADKQQAALDAKQ
                     REAQRQKNLARIAGLAGATGAPTATGTALKSAGPSPGYAGRIRARIKPNIVFTEEVQG
                     NPTAEVEVRTAPDGTIISSKLIKSSGLKSWDDAVLKAIDKTEKLPPDTDGHVPTLLLL
                     VFRPLE"
     gene            complement(4491..8054)
                     /gene="dnaE"
                     /locus_tag="PLB25_04735"
     CDS             complement(4491..8054)
                     /gene="dnaE"
                     /locus_tag="PLB25_04735"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HOE40924.1"
                     /translation="MFVHLRLHTEFSVIDGTNRIDEIVKSAAADGQPALAITDLSNLF
                     GAIKFYKEGRKRGVKPVLGAEIWLEGLGKDAAQLSRVLLLVQNHQGYLNLSELLARAW
                     TQSGSKAQASISVAWLTELNGGLICLSGAQAGPVGQALLQGDLTRAMDAATQLSGIFT
                     HRFYLELQRAGRPEDEPQVVGAVQLAQRMQLPVVATHPIQFAAPDDFEAHEARVCIAE
                     GEMLANPRRLRRFTREQHFKTAAQMAELFADVPSALANSLEIARRCSLSLVLGKPQLP
                     DFPIPAVDGVVMAADDYFRHASHEGLKERLAHLYPDPVERAKQMPGYLERLEFELGTI
                     LKMGFPGYFLIVGDFINWAKSHGCPVGPGRGSGAGSLVAYALKITDLDPLQYKLLFER
                     FLNPERVSMPDFDIDFCQGNRDRVIDYVKEKYGKNAVSQIATFGTMAARAAIRDVGRV
                     LDMSYTFCDGISKLIPNKPGVAVTLQLPPPDRKKDDKTVYATEAEPILAEREAREEDV
                     KTLLVLARKLEGLTRNIGMHAGGVLIAPGKLTDFCPLYQQPGSESAVSQYDKNDVESV
                     GLVKFDFLGLATLTILEIAREFIVSRHPGQENFAFENLPLDDPATYKLFQEGKTEAVF
                     QFESRGMQGMLRDAKPTRLEDLIALNALYRPGPMDLIPSFVARKHGREVVEYPHPLVA
                     EMLSETYGIMVYQEQVMQTAQILGGYSLGGADMLRRAMGKKDADEMARHRQIFRDGAL
                     KNDIGQEKADEVFDLMEKFAGYGFNKSHAAAYSLLAYHTAWLKVHYTAEFFCANMTVE
                     MDDTDKLKVLLEDALKMGLSFEPPDVNRGMSRFEPISDLVIRYGLSAVKGSGEQAIEA
                     IVAAREGRGVGPMGDVVGPFKSLFDFCARVDRARINKRTVEALIKAGAFDTLQRNRAS
                     LLASVDLAFEFGAAALANANQCSLFDMGGADQHGSSTQEPELVQATPWGVKETLTFEK
                     TAVGFYLSGHLFDEVAQEVRRFAKRPIAGLLDTREPQLLAGIVNDFRVINGQRGKLAL
                     FKLDDKSGVIEARADEALINAHKNLLKDDELLIVMGKQQPDRFSGGMQLTVTSIWDLE
                     QARCRFGKFLRVTLASDAKGRALDVARLLKDYPAQREPTEQGDLWRGLGVRVSLTCHG
                     DGGAASVELQLDERARFYPSNAALSSWWAQAAPGSAEIIYE"
     gene            8096..8734
                     /locus_tag="PLB25_04740"
     CDS             8096..8734
                     /locus_tag="PLB25_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE40925.1"
                     /translation="MKILVVDDHALVREGLSHVLQGLMPDEATEVLQAPNCATAFDLV
                     NAHADLDLVLLDYHLPDMNGLAALTVLGKEHPELPVVILSGLANPSIMQRVLAQGAVG
                     FITKSCLSEDLLQALRLVLSGEIYSAPEFVQAYPVEHPLVTTGPPVFTPRQEEVLHLL
                     LDGCSNREISDKLCLSDETIKNHITSILRNFGVTTRTQAALEANRWGYGKPA"
     gene            complement(9046..9600)
                     /locus_tag="PLB25_04745"
     CDS             complement(9046..9600)
                     /locus_tag="PLB25_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HOE40926.1"
                     /translation="MANIAVVFHSGYGHTLRMAQSVAEGAAADLVAIDAEGNLSDAGW
                     ATLNAADAIIMGSPTYMGSVSWQFKKFADASSKPWFSQAWKNKVFAGFTNSATMNGDK
                     LSTLHYLFTLAMQHGGIWVGTGIMPSNSKAAQRNDINFVGSFSGAMAQSPSDASPTEM
                     LPGDLETARLFGQRVAEVAAKFAA"
     gene            complement(9658..10359)
                     /locus_tag="PLB25_04750"
     CDS             complement(9658..10359)
                     /locus_tag="PLB25_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HOE40927.1"
                     /translation="MLTIRPSQARGYADHGWLKSFHSFSFADYHDPAHMGWGNLRVIN
                     EDWVAPGMGFGMHGHRDMEIVTYVISGVLAHKDSMGNVETILPGEVQRMSAGIGVRHS
                     EFSDTPKETTHLLQIWIQPNVLGVTPSYEQKTFSQADKQGRLCLVAAPDGAAGAVLIH
                     ADARLYAAVLDGEQTAGLALDSNRKCYVHLVRGHLAVNGVALSTGDAALLADESQVAL
                     TNAVDAEVLVFDLSA"
     gene            10473..>11077
                     /locus_tag="PLB25_04755"
     CDS             10473..>11077
                     /locus_tag="PLB25_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOE40928.1"
                     /translation="MQTPRNVLTPDALSMLQTIAATGSFAAAARSLGMVPSALTYRVR
                     QIEDALDVLLFDRSSRQAKLTPAGAELLQEGARLLDDIDAVANRVKRVATGWEPQFTI
                     AVDSIIAKATVMELCEHFLEQSPPTRIRLRDETLSGTLAALTSGQADLALGVADLVQG
                     NAIHTRPLGDVHFVYAVAPHHPLANAPEPLTDEMLRAHRAVA"
BASE COUNT         2270 a         3441 c         3238 g         2128 t
ORIGIN      
        1 gcggcaaatt gacaaggggc gcgatgcggc ccgggtggcg gtcgaaatgg cctgcctgct
       61 ggaaaccatc tgatgaccga atcaagccac cccgctgcca cccgcccgcc gcgccagtcc
      121 cgcaagggcc tgaccagcac cggtgtgctc aaggccgcca gcaagtcgcc gcgctcgcgg
      181 gcccgcgaat ttgccctgca ggcgctctac caggtgctgg tgggcaaaaa cgagctgtct
      241 gatgtcgatg ccttcacccg tgatctggcc gggttttcga aggccgacgt ggtgcatttt
      301 gatgcgctgc tgcagggctg cggggcccag gcgctggcgc ttgatgcctt ggtgcagccc
      361 ttgctggacc ggccactgac tgaaatctcg ccgatagagc acgcggtgat gtggatcggt
      421 gcctttgagt ttcagcattg cccggatgtg ccgtggcgtg tggtgttgaa cgagtgcatt
      481 gagctggcca aggaatttgg cggcaccgac gggcacaagt acgtcaacgc catactcaac
      541 gcgctggcaa gtacattgcg cccggctgag gtcgcggccg accgcggtgc ttcgcgcgcc
      601 tgacagtttg ccagcagcac atgaacattt cggcgcgcgc ccagcggatt gagccgtttt
      661 acgtgatgga gattgccaag gccgcccagg cactggcggc aaccgtcgcc aggtcggccc
      721 agccgatgat ttttctgaac attggcgagc ccgacttcac cgcacccata ctggtgcaag
      781 aggccgccgc caaagccatc cgtgacgggc aaacccagta caccccagcc accggtctgc
      841 ccgaactgcg cgagcgtatc agccagtggt atgccagccg ttttcaagtc gcggtgccag
      901 ccagccgcat cgtcgtgacc gccggtgcct cggcagcact gcaactggct tgcctggcgc
      961 tgatcgaggc gggcgacgag gtgctgatgc ctgaccccag ctacccgtgc aaccggcatt
     1021 ttgtggccgc cgccgatggc acggcggtgt tgatcccaac caccgcagcg cagcgtttcc
     1081 agctcagcgc cgagcaggtg gcgcaggcct ggggcgacaa aacccgtggc gtgttgctgg
     1141 catcaccgtc caacccgacc ggcacctcga ttgcctttga cgagctgcgc cggattcacc
     1201 agcaggtcag ccagcgtggc ggcatcaccc tggtcgacga gatttatctg ggcttgagcc
     1261 acgacgcgca gtttggccgg tcagcgctgg cgattgacga tcagatcatc agcatcaaca
     1321 gcttttccaa gtacttcaac atgaccggtt ggcggcttgg ctggctggtg ctgccgctgc
     1381 aactggtgcc ggtcattgag cggctggcgc aaaacctgtt tatctgcccc agcacgattt
     1441 cacaatacgc ggcgctggcc tgctttgagc ccgacagcct ggctgaattt gagcaacgcc
     1501 gggcccaatt caaggccagg cgcgactact ttattccagc cctcaatgca ttggggttga
     1561 gcgtgccagt gatgcccgac ggtgcctttt acgcctgggc cgactgcagc gcagcatgca
     1621 gcaagctcaa ggtcaatggc agttgggacc tggcgtttga gctgatgaag cgcgcccata
     1681 tcgccatcac ccccggacgt gactttggcc aggccgaaac cgcccatttc atccgcttgt
     1741 ccaccgccaa ttcaatgccg cagttgcgcg aagcggttga ccggctgcag actttgctgg
     1801 catgaacagc gtcgccagca cccaaccggc ccaggcacac aagccgtttt actggccggt
     1861 gcgcatttat tggcaagaca ccgatgctgg cggcatcgtc catcacgcca actacctcaa
     1921 ttttttcgaa cgcgcccgca ccgaatggct gcgtgcgctg ggtattggtc agcaatccct
     1981 gcgtgaaaca accggcggca tgtttgtggt cagtcaggcc aacctgagct atgtcaaaag
     2041 cgcccggctc gacgatgaac ttttgcttac aacttcactc acagaagcgg gccgggtcgc
     2101 catgacaatc cggcagcagg cgctgcgcaa gactttgggt gccaacctgc tgttgtgcga
     2161 ggcaaacctt cgcctggcct gggtcaacgc cgacggcaaa ccggccagaa ttccaccagt
     2221 tatcctaagc accttgagcc aaggcattca accttcacca ttcgtcatca atcaacaatg
     2281 aaccaagaac tctccatcct gcatctggtg ctcaatgcca gcatcgtcgt gcaagcggtg
     2341 atgctgctgc tgatcagcgt ttcggtggcc agttgggctg ccatctttcg caagctgttt
     2401 tcgctgggca aggtgaagtc gctcaacgat gcctttgaac gtgaattctg gtctggcagc
     2461 agtttgaacg acctgttttc cgctgccacc aagaacgccc gcaacgccgg gccgatggag
     2521 cgcatttttg ccagcggcat gcgtgagttt caaaaactgc gcgaacgccg catcaccgat
     2581 gccggtaccc tgatggacgg tgcccggcgt gccatgcggg ccagctttca gcgcgaaatg
     2641 gacgtggtgg aaacccattt gtcatttttg gcatcggtcg gttcggtgtc tccttatgtg
     2701 gggctgtttg gcacggtctg gggcatcatg cacgccttca ccgggctggc ggcgctcacc
     2761 caagtcacgc tggccaccgt tgcccccggc attgccgagg cgctggtggc cactgccatc
     2821 ggcctgtttg ccgcgattcc ggcggtggtg gcctacaacc gctttgcgcg cgacattgac
     2881 cgcatcgcaa tccggctgga gaccttcatt gaagagtttt ccaacatcct gcaacgcaat
     2941 gttggcgcgc aatcggcctc tggccactga aagactggag ccagccgatg ccatccatga
     3001 ccagtcgcgg acgtggccgc cgcaccatca gcgagatcaa catggtgccc ttcatcgatg
     3061 tgatgctggt gctgctgatc attttcatgg tcaccgcgcc gctgatcgcc cccagcatga
     3121 tcagcctgcc cagcgttggc aaaggcgaca aacagcccga tcaggtggtg cagatcatca
     3181 tcggcaagga cgagacgctg gccctcaagc tcaaagacca gacatcgccg gtggccttga
     3241 aggagctggt gcgcagcgtc agacaggcgc aaggcggcgt ggccaattcg gcggtggtga
     3301 tcagcgccga caaaagcgtc aagtacgaag ccgtggtgcg tgtgatggac acgctgcagc
     3361 gtgccggcgt ggcgcgcgtc ggcctgtcgg tacaacttgc caagtgagct tgggtcatgc
     3421 acgctggcgc tgacagactg gaatttgcgc cgcccgccac gcccggactg ctgcgtgcga
     3481 tggtgctggc gctgctggcg catggtctgc tgctgattgc actgacttgg ggcgtccact
     3541 ggcagcgcga tgttcaagtt gccagttatg aagccgaact ctggtctgcc ttgccgaccg
     3601 aggccgcccc ggccctggtt gaaacgccgc cgccagaacc gcctgagccg cccccaccgc
     3661 tacccccggt gccagagccg ttgccagtgc caaaaccagt gccagcaccg cccgagccca
     3721 agccggtgcc agcgcccaag ctgccggagg ttgacattgc cttgcaacag gaaaagcagc
     3781 gcctgaaaaa gcagcaggag caaaagcaag aagagcaacg ccaggacaag ctcaaggcag
     3841 agaaactcaa acttgaaaaa atcaaacaag aaaaggccga acttgagaca gccaagctca
     3901 acaagctgaa acaggaaaag ctcaagcagg agcagctcag gcaagagcag ctcaggcaag
     3961 agcagctcag gcaagagcag ctcaagcagg aaaagctcaa acaggcgcag gccgacgatg
     4021 cacagaaagc cgcagcagac aagcaacagg ccgcgctcga tgccaaacag cgagaggccc
     4081 agcggcagaa gaacctggcc agaattgccg gcctggccgg tgccaccggt gccccgactg
     4141 ccactggcac ggcgctgaaa tctgctggcc cgtcgcctgg ctacgccggg cgcatccgtg
     4201 ctcgcatcaa gcccaacatc gtctttaccg aagaggtaca gggcaacccg accgccgaag
     4261 tggaagtacg caccgcgccg gatggcacca tcatcagcag caaactgatc aaatccagcg
     4321 gactcaaatc ctgggacgat gcggtgctca aggccattga caagaccgaa aagctgccac
     4381 ccgacaccga cggtcacgta ccgaccttgc tgctgctggt tttccggcca ctcgaataag
     4441 ctacatttac cgtagctgcc agcgcttatt ccataagcgc tgcaggccga ttactcataa
     4501 attatttcgg cagagccagg tgccgcctgg gcccaccagc tcgacagcgc ggcattgctg
     4561 gggtaaaacc tggcgcgctc gtcaagctgc aactcaacac tggcagcgcc gccgtcgccg
     4621 tgacaggtca ggctcacacg cacacccagg ccgcgccaca agtcaccctg ctcggtcggc
     4681 tcgcgttgcg ccgggtagtc cttgagcagg cgtgccacat ccagcgccct gccctttgca
     4741 tcgctggcca gcgtaacccg caggaatttg ccaaagcggc agcgcgcctg ttccaggtcc
     4801 cagatgctcg tcaccgtcaa ctgcatgccg ccagagaacc ggtccggctg ctgtttgccc
     4861 atgacgatca acagttcatc gtccttgagc agattcttgt gggcgttgat cagcgcctca
     4921 tcggcgcgcg cctcgatcac gccggatttg tcgtcaagct tgaacagcgc gagtttgccg
     4981 cgctggccgt tgatgacccg aaaatcattg acgataccgg ccagcaactg cggctcgcgg
     5041 gtgtcgagca gtccggcaat tggccgcttg gcaaagcggc gcacctcttg tgccacctcg
     5101 tcaaacaggt ggcctgacaa ataaaaaccg acggcggttt tctcaaacgt cagggtctcc
     5161 ttgacgcccc agggcgtggc ttgcaccagt tccggctctt gtgtgctgga gccatgctgg
     5221 tcagcgccgc ccatgtcaaa caggctgcac tggttggcgt tggccagcgc ggcagcgcca
     5281 aactcaaagg ccagatcgac gctggccagc aggctggcgc ggttgcgctg cagggtgtca
     5341 aaggcaccgg ccttgatcag cgcttccacc gtgcgtttgt tgatgcgggc gcggtccacc
     5401 ctggcacaga agtcaaacag gctcttgaac ggcccgacca catcgcccat cggaccgaca
     5461 ccgcgtccct cgcgcgccgc cacaatcgcc tcgatcgcct gctcgccgct gcccttgacg
     5521 gcgcttaatc cataacggat gaccaggtca gaaatcggct caaagcggct catgccacgg
     5581 ttgacatctg gtggctcaaa gctcaggccc atcttcaggg catcttcgag cagcaccttg
     5641 agcttgtcgg tgtcgtccat ttccaccgtc atgttggcgc aaaagaactc ggcggtgtaa
     5701 tgcaccttga gccaggcggt gtggtaggcc agcagcgaat aagcggcagc gtgtgacttg
     5761 ttgaagccat agccggcaaa tttttccatc aggtcaaaaa cttcgtcagc cttttcctgc
     5821 ccgatgtcat ttttcagggc accgtcacga aagatctgcc ggtgccgggc catctcgtcg
     5881 gcatcttttt tacccatggc gcgacgcaac atgtcggcac cacccaatga gtagccaccc
     5941 agaatctggg cggtctgcat cacctgttcc tgataaacca tgatgccgta ggtttcagac
     6001 agcatctcgg cgaccagcgg gtgcgggtac tcaaccactt cgcgcccatg tttgcgcgcg
     6061 acaaagctgg ggatcaaatc catcgggcca gggcggtaca aggcgttcag cgcgatcagg
     6121 tcttccagcc gcgtcggctt ggcatcgcgc agcatgccct gcatgccgcg tgattcaaac
     6181 tggaacaccg cctcggtctt gccttcctga aacaacttgt aggtggcagg gtcatcaagc
     6241 ggcaggttct caaaggcaaa attttcctgc cctgggtgac ggctgacaat gaattcgcgg
     6301 gcaatttcca gaatcgtcag cgtggccagg cccaaaaagt caaacttgac caggccgacc
     6361 gattccacat cgtttttgtc gtactggctg acggccgatt cgctgccggg ctgctggtag
     6421 agcgggcaaa agtcggtcag tttccccggc gcgatcagca cgccgccggc gtgcatgccg
     6481 atgttgcggg tcaggccttc gagcttgcgc gccagcacca gcaaggtctt gacatcttct
     6541 tcacgcgctt cgcgctcggc cagaatcggc tcggcctcgg tggcataaac ggtcttgtcg
     6601 tcctttttgc gatccggtgg cggcagttgc agggtaaccg ccacacccgg cttgttggga
     6661 atcagcttgc tgatgccgtc gcaaaaggtg tagctcatgt cgagcacccg accgacatcg
     6721 cgaatcgccg cgcgcgctgc catggtgcca aaagtggcaa tctggctgac cgcattttta
     6781 ccgtattttt ctttgacgta gtcgatcaca cggtcgcggt tgccctgaca gaagtcaatg
     6841 tcgaagtcgg gcatcgagac ccgctcgggg ttcaaaaatc gctcaaacag cagtttgtat
     6901 tgcaacgggt cgaggtcggt gatcttcagt gcgtaagcca ccagcgagcc agcccccgag
     6961 ccccgccccg gcccaaccgg gcaaccatgg cttttggccc agttgatgaa gtcgccgacg
     7021 atcagaaagt agccgggaaa acccatcttc agaatggtgc ccagctcgaa ttcgagccgt
     7081 tccaggtagc ctggcatctg ctttgcccgc tcgaccgggt cggggtacag gtgcgccagg
     7141 cgctctttca gcccttcgtg cgaggcatgg cgaaagtaat cgtccgctgc catcaccaca
     7201 ccatcgaccg ccggaatcgg aaaatccggc aactgcggct tgcccagcac cagactcaag
     7261 ctgcagcgcc tggcaatctc caggctgttg gccaatgcag atggcacatc agcaaacagc
     7321 tcggccatct gggctgcggt tttgaagtgc tgctcgcggg taaagcggcg cagccgacgc
     7381 gggttggcca gcatttcgcc ctcggcaatg cagacgcggg cctcgtgggc ttcaaaatca
     7441 tccggtgcgg caaactggat cgggtgcgtg gccaccaccg ggagctgcat gcgctgcgcc
     7501 aattgcactg cgccgaccac ttgaggctcg tcttctggtc ggccagcgcg ctgcagctcc
     7561 aggtaaaagc ggtgggtgaa gatgcccgag agttgcgttg ccgcatccat cgcccgcgtc
     7621 aggtcgccct gcagcaaagc ctgcccgacc ggcccggcct gcgcacccga caggcaaatc
     7681 aagccgccat tgagttcagt cagccaggct acgctgatgc tggcttgcgc cttggagccg
     7741 ctttgggtcc aggcgcgcgc cagcagttcc gacaggttca ggtagccctg gtggttttgc
     7801 accagcaaca gcacgcgtga gagttgcgcc gcatccttgc caagcccctc cagccagatt
     7861 tcggcaccca aaactggctt gacaccgcgc ttgcgccctt ctttgtagaa cttgatggca
     7921 ccaaacaggt tgctcaaatc ggtgatggcc aaggcgggct ggccatcagc ggctgcagat
     7981 ttgacaatct cgtcgatgcg gttggtgccg tcaatgacgg aaaattcggt gtgcagacgc
     8041 aagtggacaa acatatatgg gctatattgt aggaactgca gttggagtga atgacgtgaa
     8101 gatcctggtg gttgatgacc atgccttggt aagggaaggg ctaagccatg tgttgcaggg
     8161 gctgatgcca gatgaggcca ccgaagtgct gcaggcaccc aattgcgcaa ctgcgtttga
     8221 tttggtcaat gcccacgcgg accttgatct ggtgctgctg gactaccacc tgccagacat
     8281 gaatggcctg gcagcgctga ctgtgttggg caaagaacat cccgagctgc cggtcgtcat
     8341 tctgtctggc ttggccaacc caagcatcat gcagcgtgtg ctggcgcagg gagcggtcgg
     8401 cttcatcacc aaatcatgct tgagcgagga cttgttgcag gctttgcgac tggtgctcag
     8461 cggcgagatc tattcagcac cagagttcgt tcaggcatac ccggttgagc accccttggt
     8521 cacgacaggt ccaccggtat tcacgccgcg ccaggaagaa gtgctgcacc tgctgcttga
     8581 cggctgcagc aaccgggaaa tcagcgacaa gctctgcctg tcggatgaaa ccatcaaaaa
     8641 ccacatcacc agcatcttgc gcaattttgg agtcacgacc cgcacgcagg cggcgctgga
     8701 ggccaaccgc tggggctatg gcaaacctgc atagtttgca acctgatccc ggctattgaa
     8761 cgcccaagtt tgctctgctg ctgtgctctc gcgggactgc cacatgtgcc ctgaagacaa
     8821 atttgctcgg tgctcctgaa gtaacaactc gtcatcttgc agcaacaagc tgcaggccgt
     8881 gccctcggca agcggcaaag cgctacagcc cccgccaagg tgcccgcagc gatgccaaca
     8941 acaccgtcag gcaagcctga aaacagcctg aaatgcttta taaaacatag caggctacgc
     9001 aattcccata agggctagag gcctataacc ttcacatttt agtgatcaag cggcgaactt
     9061 ggctgccact tcggccacgc gctgaccaaa cagccgggcg gtttccaggt cgcccggcaa
     9121 catttcagtc ggcgaagcat ctgatggcga ctgggccatg gcaccgctga aggagccgac
     9181 aaaattgatg tcgttgcgct gggcggcctt gctgttgctt ggcattattc ctgtgccaac
     9241 ccagatgccg ccgtgctgca tggccagcgt gaacaggtaa tgcaaggtcg acagcttgtc
     9301 gccattcatc gtggcactgt tggtaaaacc ggcaaacacc ttgtttttcc aggcctggct
     9361 gaaccagggt ttgctcgatg cgtcagcaaa cttcttgaac tgccagctca ctgagcccat
     9421 gtaggtgggg ctgcccatga tgatggcatc ggcggcgttg agcgtggccc atccggcatc
     9481 tgacagattg ccttcggcat caatggcgac caggtcggca gcagcgccct cggccaccga
     9541 ttgcgccatg cgcagggtgt ggccgtagcc agaatgaaaa acgacagcaa tattggccat
     9601 agtgaaaact ccttgtggtt tggtgaaaaa aatgggggtg aaaattatcc agaaaattca
     9661 ggcggacaaa tcaaaaacca gcacctcggc gtccactgcg ttggtcagag cgacttgcga
     9721 ctcgtctgcc agcagcgcgg catcgccggt actcaaggcc acgccgttga ccgccaggtg
     9781 gccgcgcacc agatgcacat agcattttcg attggagtcc agggccaacc ctgcggtctg
     9841 ttccccatcc aacacagcgg catacagccg cgcatcggca tggatcaata ccgcaccggc
     9901 cgcaccatcg ggagcggcca ccagacacag ccgaccctgt ttatcagcct gtgaaaacgt
     9961 tttctgctca tagctgggcg tgacaccgag cacattgggc tgaatccaga tttgcagcag
    10021 atgggtggtt tccttggggg tgtcgctgaa ctcactgtgc cgcacaccga tgccggcact
    10081 catgcgctgc acctcaccgg gcagaatcgt ctcgacgttg cccatgctgt ccttgtgcgc
    10141 caggacaccc gagatcacat aggtgacgat ttccatgtcg cgatggccgt gcatgccaaa
    10201 acccatgccc ggcgcaaccc aatcttcgtt gatgacccgc agattgcccc accccatgtg
    10261 cgcagggtca tggtaatcag cgaacgaaaa gctgtgaaac gacttgagcc agccgtggtc
    10321 ggcgtagccg cgtgcttgtg agggtctgat ggtcaacatg ctgaagaact ccaaaattga
    10381 acaatgcagt gactgtagcg tcaccaacga atcggttttg actgcggatt tgatggcatc
    10441 attcaaatca ttcgaactca aatgcccgat tcatgcaaac accccgcaat gttctgacac
    10501 ccgatgccct gtccatgctg caaaccattg ccgcaaccgg cagtttcgcc gccgccgcgc
    10561 gctcgcttgg catggtgccc agcgccctga cctaccgggt tcgccagatt gaagacgcgc
    10621 tcgacgtgct gctgtttgac cgcagttcgc gccaggccaa gctgacaccg gcgggtgccg
    10681 aactgctgca agagggcgcg cgcttgcttg acgacatcga tgcggtggca aaccgggtca
    10741 agcgcgtggc cactggctgg gagccgcagt tcaccatcgc cgtcgacagc atcatcgcca
    10801 aagccacggt gatggaattg tgcgaacatt ttctggaaca aagcccgccg acccgcattc
    10861 ggctacgcga tgagaccctg tcgggcacgc ttgccgccct gaccagcggc caggccgatc
    10921 tggcgcttgg cgtggccgac ctggtgcaag gcaacgccat ccacaccagg ccgctgggcg
    10981 atgtgcactt tgtttatgcg gtggcacccc atcatccgct ggccaacgcg cccgagcctt
    11041 tgaccgacga gatgctgcgc gcgcaccgcg ccgtggc
//
LOCUS       DAOSLL010000072        10884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_5941_length_10884_cov_5.222366, whole genome shotgun sequence.
ACCESSION   DAOSLL010000072 DAOSLL010000000
VERSION     DAOSLL010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10884
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5941_length_10884_cov_5.222366"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="PLB25_04760"
     CDS             complement(<1..271)
                     /locus_tag="PLB25_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 family protein"
                     /protein_id="HOE40929.1"
                     /translation="MSDSEQVITDTVAWLERAVIRLNLCPFAKSVHVKGLVHYAVSQA
                     TSPKAVLNDLVFELKSLAAVDEKARDTTLLIVPRCLHDFLDFNDFL"
     gene            complement(355..1539)
                     /locus_tag="PLB25_04765"
     CDS             complement(355..1539)
                     /locus_tag="PLB25_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HOE40930.1"
                     /translation="MQLACYACDPAHSRGRRFAQPPAPTRTEYQRDRDRIVHSTAFRR
                     LVYKTQVFLNHEGDLFRTRLTHSLEVAQLARSIARSLGLNEDLVETIALAHDLGHTPF
                     GHAGQDALNDCMAKHGGFEHNLQSLRVVDHLEERYPGFDGLNLTFETREGILKHCSMQ
                     HAQQLERAEPGGVAQRFLQRTQPSLEAQLCNLADEIAYNAHDIDDGVRSGLITLAQLQ
                     DVALFDEYKCQTLADYPHLQDKTQGRRLLYESIRRMLSNQVYDVIAATQSALDATHPM
                     NADAARQLPPLVQFGTAMRDKSRELKAFLLQQLYRHPQVMQTTGQARQVVRDLFAAYS
                     AAPDEMSESHQQRLRLLPSDAAAATDTHAVTARLVADYIAGMTDRFAAREHQRLTGLQ
                     LL"
     gene            complement(1606..2712)
                     /gene="aroB"
                     /locus_tag="PLB25_04770"
     CDS             complement(1606..2712)
                     /gene="aroB"
                     /locus_tag="PLB25_04770"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HOE40931.1"
                     /translation="MKSPDLQTVAIDLADRSYNIAIRGSLFDNPLSYAELPKAAAALI
                     VTNTTVGPLYAERLQRALATKFAQIHTVVLPDGEQYKTWQSLNLIFDALLGHGCDRKT
                     TLFALGGGVVGDMAGFAAASFMRGVPFVQVPTTLLAQVDSSVGGKTAINHPLGKNMIG
                     AFYQPLKVVCDLDTLKTLPKRELSAGLAEVIKYGPIADIDFMAWIEANLDAILARDAV
                     ALAHVVKRSCEIKAWVVGQDEREAGLRAILNFGHTFGHAIEAGLGFGAWLHGEAVGCG
                     MVMAAQLSARLGLVDAFFVNRLTQLIAKAGLPVKAPVLPGIDNAGRYLELMQLDKKAE
                     AGEIKFVLIDKPGRALVRSAPTAMVRQVIDACCA"
     gene            complement(2833..8759)
                     /locus_tag="PLB25_04775"
                     /pseudo
     CDS             complement(2833..8759)
                     /locus_tag="PLB25_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
     gene            complement(8797..10854)
                     /locus_tag="PLB25_04780"
     CDS             complement(8797..10854)
                     /locus_tag="PLB25_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE40932.1"
                     /translation="MLRFLGKKTVAGHQRVLSAILIGCLLLLAALTALGLRQSGQAAG
                     QLNATGQALMQSQRLAKLVTQALVGDAKAFADLAASAQVLAKSVHGLMEGDSDMGLAA
                     VGEVDRRILEKAEPLADRAEKNAATVVSQKKALTQTAAALQAVQRQSGALLELSQAAA
                     TLKFQRQASVAEISAASQLGMLTQRIGKSIAEFLSIDGVVPASVTLLGKDLETFKQIA
                     AGLLDGRVELGLAASGDAATREKLQALIVLFEETSTQATAVVTNLPALTSARTAQVAI
                     LADSELLRQHLEELQASLSGQTALGGATLVALTVLALLALLSAAALFYVQLLDSRQRQ
                     VDAEANKIEAQAQEQDAKRVNDANQAAILRLMNELQLVAEGDLTQQATVTEDITGAIA
                     DSVNYTVEELRSLVGSVQNTVAKVATTTADVDVTSTELLAASNEQLHEIRETGRSVVD
                     MATRINQVSNQAQESADVARQSLRAAEVGLHAVQSAIGGMNTIRDQIQDTSKRIKRLG
                     ESSQEIGEITELISDITEQTNVLALNAAIQAASAGDAGRGFSVVAEEVQRLAERSADA
                     TRQIAALVKAIQTDTQDAVGAMERSTHGVVEGAKLSDNAGAALSQIDRVSRHLAELIE
                     HISASTLQEANLANELAGNIQHIFAVTEQTGEGTRSTATQVRELSKMAEELRQSVARF
                     RIA"
BASE COUNT         2281 a         3418 c         3076 g         2109 t
ORIGIN      
        1 ccagaaagtc attgaaatct agaaaatcat gcagacacct cggcactatc aatagcgtag
       61 tgtcccgcgc tttctcatca acggctgcaa ggctttttag ctcaaaaacc aggtcattga
      121 gcacggcttt tggtgaagtc gcctggctga ccgcgtaatg caccagaccc ttgacatgta
      181 cgcttttggc aaacgggcac aggttcagcc ggatcaccgc ccgctccagc caggccacgg
      241 tgtcggttat cacttgttca gaatcgctca tcctttttac tccagggaaa accggacttg
      301 ggcaacgaat ccctggcagc agcgcgggct gcctgagaca attttgcgca agtcttacaa
      361 cagttgcaag ccagtcagac gctggtgctc gcgcgccgca aaccggtcgg tcatgccggc
      421 aatgtagtcg gccaccagcc tggccgtcac agcgtgggtg tcggttgccg cagcggcatc
      481 tgagggcagc aaacgcagcc gttgctggtg gctttccgac atttcatccg gcgcggcgct
      541 gtaggctgca aacaggtcac gcaccacttg ccgggcctgc ccggtggtct gcatcacttg
      601 cggatgccgg taaagctgct gcagcaaaaa ggccttcaat tcccgcgact tgtcacgcat
      661 ggcagtgcca aattgcacca gcggcggcaa ctgccgcgct gcgtcggcat tcatcgggtg
      721 ggtcgcgtcc agcgcacttt gcgtggcggc gatcacatcg taaacctggt tgctgagcat
      781 gcggcgtatc gactcgtata gcagccggcg accctgcgtt ttgtcttgca gatgcgggta
      841 gtcggcgagg gtctggcatt tgtattcatc aaacagcgcg acatcctgca attgcgccag
      901 cgtgatcagg ccggagcgca caccgtcgtc gatgtcatgc gcgttgtagg cgatctcgtc
      961 ggccaggttg cacagttgtg cctccaggct gggctgggtg cgctgcagaa aacgctgcgc
     1021 cacgccgccc ggctcggcgc gctcaagttg ctgcgcgtgc tgcatcgagc aatgtttgag
     1081 gatgccctcg cgcgtctcaa acgtcaggtt caggccgtca aacccagggt agcgttcttc
     1141 cagatggtcc accacccgca ggctttgcag gttgtgctca aaaccgccgt gcttcgccat
     1201 gcagtcgttc agggcatcct gcccggcgtg gccaaacggc gtgtgcccga ggtcatgcgc
     1261 cagcgcgatg gtttccacca ggtcctcgtt gagcccaagc gaacgcgcga ttgaccgtgc
     1321 caactgcgcg acttccagcg aatgcgtgag ccgggtgcga aacagatcac cttcgtggtt
     1381 cagaaacacc tgggttttat agaccagccg ccgaaacgcc gtgctgtgca caatacggtc
     1441 acggtcgcgc tggtattcgg tgcgggttgg cgcaggcggc tgggcaaagc gccgaccacg
     1501 gctgtgcgcc gggtcgcagg cgtaacaggc aagctgcatt ttatgcaata aaaatggctc
     1561 tagcccttac cacacctgtg ctggcagcta tgctttttat gatcatcagg cgcagcaagc
     1621 gtcaatcacc tgccgcacca tggcagtcgg cgcgctgcga accagggccc gccccggttt
     1681 gtcgatcagg acaaatttga tttcaccggc ctcagccttt ttgtccaact gcatcaactc
     1741 aaggtagcgg ccagcgttgt cgatacccgg caaaaccggc gccttcaccg gcaaaccggc
     1801 tttggcaatc aactgcgtca ggcggttgac aaaaaaagca tcgaccaggc cgaggcgtgc
     1861 cgacaactgc gccgccatca ccatgccgca gccaactgcc tccccatgca gccaggcccc
     1921 aaaacccagg ccagcttcaa tggcgtgacc aaaggtatgg ccaaagttca aaatggcacg
     1981 cagtccggcc tcgcgttcgt cctgccccac cacccatgcc ttgatttcgc aactgcgttt
     2041 gaccacgtgc gccagcgcca cggcatcgcg tgccagaatg gcatccaggt tggcctcgat
     2101 ccaggccatg aagtcgatgt cggcgatcgg gccgtatttg atgacctcgg ccagcccggc
     2161 actgagttcc cgctttggca aggtcttgag tgtgtcaagg tcacatacca ctttcaacgg
     2221 ttggtaaaac gcgccgatca tgttcttgcc aagcgggtga ttgatggcag tcttgccacc
     2281 caccgacgaa tcgacctgcg ccagcagcgt ggttggcacc tgcacaaaag gcacgccacg
     2341 catgaaactg gccgccgcaa aaccggccat atcgccgacc acaccgccgc ccagggcaaa
     2401 cagcgtggtt tttcggtcgc agccatggcc aagcaaggca tcaaagatca ggttcagact
     2461 ttgccaggtc ttgtactgtt cgccatcagg cagcaccacc gtgtggattt gggcgaactt
     2521 ggttgccagc gcgcgctgca aacgctctgc atacagcggg ccaacggtgg tgttggtgac
     2581 aatcagcgct gctgccgcct tgggcaattc agcgtaactc agcgggttgt caaaaagcga
     2641 gccacgaatg gcgatgttgt aactgcgatc agccaggtca atggccacgg tctggaggtc
     2701 tggggatttc atgccacaga gtttagactg aagtgccagc cagcaggcgc gatctggcag
     2761 tgccggttcg aatcccgaga aactatcaaa tatatagctg gctacacaat gccatcaagg
     2821 gctacaggcc aatcatgccg tctctttggc ggcaatttcg gcattgcaat agccgcgcac
     2881 caacgaaatc agttgatcct ctgggtaggg tttgcccagg tagtgatcaa ccttgagtgt
     2941 gcgcgcatac tcgcggtgct tctcggccag gcgcgaggtg atcatgatga tcggcatgcc
     3001 ttgcaggttt tcatcggcgc ggatattgcg caccagatca aagccatcca tgcgtggcat
     3061 ctcgatatcg gtcagcacca ccgagggctt ctcaatctgg agcacctcca gtgcatgcaa
     3121 gccgtcgttg gccagcgcca cccggaagcc ttcgcgcttg agcaggcgct gcgtgacccg
     3181 gcgcacggtg atggagtcgt ccaccaccag caccagcggc aactgcgcag cgggtgtcgg
     3241 ctgggtttgc ccaggcaccg cagcaccctt gccagcctgt acctgggcac ccgaactggc
     3301 aaaagcatag gcctgcccgg agtagacgtg ggccaaagcg accgggttgt agatcaatac
     3361 cacagccccg gaggccagca ccgacatgcc tgccaggccc ggcaggcgtg acagttgcgg
     3421 ccccaggttt ttcaccactg cctcctggtt gcccagcact tcatcgacat gcagggcaac
     3481 acgctgcccg gcacttctga acaccgcgac gaccgccatc ttgccccgca catcggtgct
     3541 gactggagaa atctgcaaca gcgcaccggc ccagaaaaaa gccaggcttt ccccgttgta
     3601 gtcaaaatgg cggctctggt aagccgcatc aagctctgcc accgggatac gacgcaccat
     3661 ctcgaccagg ttggacggca cgccaatcac cacatcgccc atgcgcagca gcaccacctg
     3721 ggtcaccgcc atggtcaggg gcaatacaag cttgaactgc gtgccttgac ccggttgggt
     3781 actgagctca atgtggccac ccaggccatt gacctcggtg cgcaccacat ccatgccaat
     3841 gccacgaccc gccatttcac tcacttcagc ggcagtcgac aggccagagg taaagatcag
     3901 ttcagcaatt tgctggtctg acagtatttg atcggacgtg atctggcctt tttcaagggc
     3961 cttttcacga atgcgggcca ggttgagacc ggcaccatcg tcgtgaacag tcaccacaac
     4021 atcgttactt tgctgtacca ctgtgatgtt gatggcacca gttggtggct ttccggccgc
     4081 cgcacgaacc gccgccgact cgatgccgtg gcccaccgcg ttgcgcaaca aatgctcaaa
     4141 ggccggtgcg gcacgctcca gcacaccgcg atccatgtcc agcgagccac ccactatgtc
     4201 gagcttgacc ggcttgtcaa agtctttggc tgcctggcga accacgccgt acaggcgatc
     4261 agcaacgctc tcgaaatcga ccatgcgggt gcgcagcaaa tcccgctgca acgcgcgtgt
     4321 ctggcgcgcc tgggcaacca ggtcgtcctc gaccccggca accgtctcct gcaggctgtg
     4381 ctgcagggtc gccacatcat gcactgattc ggccatcatg cgggtaagct cctgcacgcg
     4441 ggtgaatcga tcaaactcca gtgggtcaaa gccctgtgtc gaatccttgg tttgcgccag
     4501 tcgcgactgc atttgcgact cggactgcag ctccatgtcg cgcaactgat gccgcaggcg
     4561 atcaaggttg ccggtgagat cgctcagggc accacgcaac tgactcaggc gcgactccag
     4621 ccgggcgcgg ctgatcatga cctcgccggc ctggttcacc atgcgctcga gcaaatggga
     4681 tttgacccgg gtggtatgac tcggcgagcg gcgcgccagc attggcggtg ccgcagcggg
     4741 cgcaaccggg gcagactcgg tcaactttgc cggtgtggca ttgtcagccg ccggaagaag
     4801 ttgaggtccc tctggcaaca ccagcgctgc gacgggtgtg gctcgcaggg catcaaacag
     4861 gcagaaatag catccagccg cgccagcaaa gggtcaatct gtgcgctgct ggcggtttca
     4921 ctgtcaattt cctcggcagc agattccatc cggtgcgcca tttcacccaa gcgcatggca
     4981 cctgccagac gtgcactgcc tttgagcgtg tgcaacaccc gcaacccctc acgccgggca
     5041 gcggcatcgt ctggttgcgc actccattgc cgcaacgcgc tgccaagctt gggcagcagt
     5101 tcgagcgctt cttcctcgaa gatcggaaac agatcgacat cgagcgtatc gcgggccaga
     5161 atttcatctt cagattcagt caacgttgat gccggatcga cagcgtcggc ttcgactgcc
     5221 agttcaggca ccaggcccaa ctcagggacc ggctcaggct tgacaggctc cacatcgggg
     5281 gtcaaatcaa caaggtccag atcatcgggc aacaggtcgt caaacaaatc agtcctgggc
     5341 ccggacgacg gcgaggtgct gacatcaatg tcgagaatcg cccgcaatgc tgccaaatgc
     5401 gctggatcag gccgcttcag gaaaccggct gcaaactggt gcaacaaacg gcgaatagct
     5461 tcggccactt caacaaacaa accagcgtgc tcggccaaag caggcccgcc gagctgcgta
     5521 tgctccagcg catactcaag ggcccgggcc aattcggaca atcccatgaa gccaacagtc
     5581 gaagaactgc cggccagcga atgggccatc gccacggttg agtcctccag gggctgatcc
     5641 ggttcaaatg accactcggt caactcagtc agcaaacgcc gagaccattc atcggcctcg
     5701 ttgaggtaaa cgttgtacaa ggcaaggccg atgcgcagtg agtcaatgac cttgacctga
     5761 tcttcaggta ccggttctgg ctcaagaact ggttctgcct caagaactgg ttctgcctca
     5821 agaactggtt ctggctcaac aactggtgct ggctcaacaa ctggtactgg cgctgatgcc
     5881 tcgtcttgcg ggaacgcagc catcaggtcc gccaacggat ccggttcggc taccagggct
     5941 ggcgccacgg tctgccagtc tggcggcaca acctggtccg gcaggtcggg tgcggtcccg
     6001 gcaacgggtg ccagcacatc gtcgaaatcc ggcgacgctt cggccggtgt tgataccgct
     6061 ggcaactctt gtggttgttc ttgcaacacc tcgtcaaatg aaaagtccaa caggtccgcg
     6121 cccggcaccg cctgatctgc cagcccgagc gaccgggtgt cgctaaagtc gatcatctgc
     6181 gtggcagaaa agtcctgctg cggttcaggt gattcatcga aatcagccaa cgggaagtcc
     6241 agcggcaggt ctgccgacgg cactggcacg tctggcgttt caactgcttg tggtggggtg
     6301 accggctcaa gcacttcaac acgatcagcc ggcttgtcca tgaccggcaa cagcaggtca
     6361 ccagcaacat tctccaggcg caatcgatca gccgccacgc ggaaaggcaa ggcacgccag
     6421 ggcgcatcgg tgccggatgc aatgtcttca acccagcgct caaaacccgc aatggcctgg
     6481 cctgaaagct tcagcagtgc gggcgtgacc ggtagttgct gtggtaacca ggcgttaagc
     6541 aactgctcca gtgcccatgc agcctcacca aactcgttaa gccccaccat gcgagaactg
     6601 cccttgagcg tgtggaaaca ccgccgcaag tgggtctgtt gctcctgatc agcgccgttg
     6661 tcttgcaagg aggtgatcgc atcaaacccg ctttggacaa cttcgcgtgc ctcatctaga
     6721 aagatgttgc gcaattcggc atcatcatcc tcagcttcgg gcatgggcga cagcgccggg
     6781 gccggggccg gtggggccaa ctcgcgcacc ggcagcacaa ctggctcgac tggcaggaca
     6841 atgggttgca tcggcaagct cactggcact tcaggcggcg ctacggttga tgccgcatgc
     6901 acaggtggca ttggtaattt gtcagagagc gcttgcgggg ctacctgggc aggcactggc
     6961 tcaggggcgg ctggcggcat cactggcgat tcagcactgg ccttttggcg acccatcagg
     7021 gaacggaact cacccagctc gtcgtcataa acaaacagct tttttgccaa ctcccgctgg
     7081 tagctcagca tgtcaatcaa aaaaccaagc gcacccaggc tgttgccgag cttttcaaaa
     7141 ccactgtccg ggccgggagc tgccgtgtca accagaaagc gctcaacact gtcgcgcatg
     7201 cgcagtgccg ccagcgccgc ctggtcgagc cccaaaacag aaaacactcc gcgcatctgg
     7261 gacagttttc cgggtacttc gtgcaaaggt gtcttgtcat cagggcgacg gaaataagcg
     7321 tccagcgatg attcaacctc gctcaaggtg gttcgcaact catcgaccac gctgcccatg
     7381 gtctggcggt cgctgacacg gcgataaagc tcctccatcc actcgtcgag tggctcgggc
     7441 tgggttccac tcacaacctg gtcgagccgc tgcgccagcc ggttgccacg ctgcaccatc
     7501 agttcgttgg tgggatcaag gtcttcgtaa gcgggctcca gatagagtat cgcagtcgcc
     7561 acttccatgg ccagggatgg caatggggcc ctgccagatc ggttcaccgc gtccaacgca
     7621 cgcgtcagcg cttttgccag ttcgctactt tccggatgca attttgtgat cgaatcagcc
     7681 accaggctga actgatcaac tgctgtcttg atgcgactga tatcaccacc tgacaacata
     7741 gaccaggttt cagttgctgc agcaatgcgc tttcgcgctt gcgccagcac cgccgggtca
     7801 aagcgcccga actgggcctt gttgtaattg accttggctg gctgaccaaa accataggcg
     7861 agctgaaccg cgcgcagcgc ggctgcctgc tgccccggca aaggcactgc ctgggcacag
     7921 aaaaaaagca gatccagcgc cagccgctcg gaaatttccg gctcgccccg cgccagcgtg
     7981 cgatattgca acagcacgcg tgacgcagca cgcttggcat aaatgtcaga tgagcacagt
     8041 cccagcgcca ccgcctcaaa ataggcggct gacacagccc acagcacccg tggatgcagt
     8101 tcggattgcc cggcggcaaa gcccagacag gtatcacaca gggcacgcgc agacggagca
     8161 tgaccggttt tgacaacatt cagaatgtag ccgtcaatac gcgagcggac cgtagcgtca
     8221 taaggcaggg gtgtcactgc cgctggcaac gcaacatcca cccagcgcca cgccatcggc
     8281 cacaggtcgg ccgggtgcac ccggtcctca ccgatcaggt ccaacacatc gcggtattga
     8341 ggaaacagcg ccaccgacga aagcgacttg cccttgagaa taccctccag ataatcagtc
     8401 agggcaaagc tggcgcgctc caccttgagc gctgcctcat cgctgcaaaa ttccggcttt
     8461 tgcacaaact tctgcaccaa agtctccatc gcgcgcagca tcttggcggg cacttccagc
     8521 ccgaccatct ccagtgcccc caccgcctgg tgcaattgct ggcgggcaat gcgcaactgg
     8581 ctgccatcaa gcgcgccaag atcagaaccc cgggacagtt ctgcgtcccg aacaaaacgc
     8641 cgaagtgcgt tggttgcacc atcgagcgac ttgcgcaatt catcgagcac ccacgccaaa
     8701 gggcccagat cggactcaaa gcgtgcgccc tcacccatca ctgcagcttg agcttgcatg
     8761 gcttaatccg tcaaagagtg gttgaaatag agcacatcat gcaatacgga aacgcgccac
     8821 cgactgacgc aactcctcag ccatcttgga gagttcacgt acttgggtag ccgtcgagcg
     8881 agtgccctcg ccagtctgct cggttacggc aaagatgtgc tggatgttgc cagccaattc
     8941 attggccagg ttggcctctt gcaaggtgga agcagaaatg tgttcaatca gttccgccag
     9001 gtggcgcgaa acccggtcaa tctggctgag cgcggcgcct gcgttatcag acagtttggc
     9061 accctcgacc acaccatggg tggaacgctc catggcacct accgcgtcct gggtatctgt
     9121 ctgaattgcc ttcaccagag cggcaatctg gcgggtcgcg tcagccgaac gttcagccag
     9181 acgctgcact tcttctgcca ccaccgaaaa accgcgcccg gcatcgccgg ctgaagcggc
     9241 ctggatggca gcgttgagcg ccagcacatt ggtctgctcg gtgatgtcag aaatcagctc
     9301 ggtgatttca ccgatttcct gcgacgactc gcccaggcgc ttgatacgtt tggaggtgtc
     9361 ctgaatctgg tcgcgaatag tgttcatgcc gccaatggcg ctttggactg cgtgcagacc
     9421 aacttcagcc gcacgcaagg attgcctcgc cacgtcggcg gactcttgcg cctggttgga
     9481 aacctgattg atgcgggttg ccatgtccac cacggagcgc ccagtttcac ggatttcgtg
     9541 caattgttca ttcgaggctg caagcaactc ggttgatgtc acatccacat cagcggtggt
     9601 ggtggccacc ttggccacgg tgttttgcac actgccaacc aacgagcgca actcttcgac
     9661 tgtgtaattg acagagtctg caatggcacc ggtgatgtct tcggtgacag tggcctgctg
     9721 cgtcaggtcg ccttctgcca ccaactgcaa ttcgttcatc aaacgcaaga tggcagcctg
     9781 gttggcatca ttgacccgct tggcatcctg ctcctgggct tgcgcttcga tcttgttcgc
     9841 ctcagcgtca acttggcgct gtcggctgtc aagcaattgc acgtaaaaca acgcagcagc
     9901 gctgagcagc gccagcaacg cgagcacggt cagcgcaacc agcgtagcgc cacccagtgc
     9961 agtttgcccc gacagcgagg cctgcaattc ttccaggtgc tggcgtaaca actcactgtc
    10021 cgccaggatc gcgacctgag cggtgcgtgc cgatgtcaaa gccggcaagt tagtcaccac
    10081 ggcggttgcc tgagtgctgg tttcttcaaa cagcacaatc aacgcctgaa gtttttcacg
    10141 ggttgccgcg tcacctgatg cggccaggcc aagctcgacc cggccatcca gcagacccgc
    10201 agcgatttgc ttgaaggttt ccaaatcttt gccaagcaga gtcaccgatg ccggaacaac
    10261 cccgtcaatg ctcaaaaatt cggcaattga cttgccaatg cgctgggtca gcatgcccaa
    10321 ctgactggcc gctgaaatct cggccaccga tgcctggcgc tggaatttca gtgtcgctgc
    10381 cgcctgcgac agttccagca gagcgcccga ctggcgctgt accgcctgca gggcggcagc
    10441 ggtttgggtc agtgcctttt tttggctcac caccgtagct gcattttttt cagcccgatc
    10501 agccagcggc tctgccttct ccagaatacg ccgatcaacc tcacccacag cagccaggcc
    10561 catgtccgag tcaccttcca tcaagccgtg gaccgacttg gccagtactt gcgcacttgc
    10621 tgccaggtcg gcaaaggctt tggcatcacc caccaacgct tgcgtgacca acttggccag
    10681 acgctgcgac tgcatcaggg cctgcccggt ggcgttcaat tgcccagcag cctgaccact
    10741 ttggcgcaag cccagagccg tcagggctgc cagcaacagc agacagccaa tcaatatggc
    10801 cgacaacacc cgttgatgtc cggcgaccgt cttcttaccc aggaagcgca acacgatctg
    10861 atctgttcca gcctgagcga cacc
//
LOCUS       DAOSLL010000073        10770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6008_length_10770_cov_4.869062, whole genome shotgun sequence.
ACCESSION   DAOSLL010000073 DAOSLL010000000
VERSION     DAOSLL010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10770
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6008_length_10770_cov_4.869062"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(51..1145)
                     /gene="nifB"
                     /locus_tag="PLB25_04785"
                     /pseudo
     CDS             complement(51..1145)
                     /gene="nifB"
                     /locus_tag="PLB25_04785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491842.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase cofactor biosynthesis protein NifB"
     gene            complement(1288..1476)
                     /locus_tag="PLB25_04790"
     CDS             complement(1288..1476)
                     /locus_tag="PLB25_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40933.1"
                     /translation="MDLGITCRTDRAEMPAWLLSGKAFNSANPLNAAATGSVCLAEHA
                     RFVACVGLICQMLPLSHH"
     gene            complement(1951..2691)
                     /locus_tag="PLB25_04795"
     CDS             complement(1951..2691)
                     /locus_tag="PLB25_04795"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015011925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE40934.1"
                     /translation="MSRFTLDRLPWPLPAALVWLAAWAVFITLAESRLGILVALAGAC
                     VLGVAASVLGASWWRRLMIALGFPLSLAVSGAVSLPAWSWLLSLLLLLLIYPLNAWRD
                     APLFPTPKNALKELAAHVPLAQHALILDAGCGLGDGLKALRGAYPQARLHGLEWSWPL
                     RALCALRCPWARVRQGDIWRADWSAYDLVYLFQRPESMPRAGNKARAELKPGAWLISL
                     EFQATALQAQMVLQAPDGRPVWLYQMPC"
     gene            complement(2951..3937)
                     /locus_tag="PLB25_04800"
     CDS             complement(2951..3937)
                     /locus_tag="PLB25_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HOE40935.1"
                     /translation="MVTKKPKGLGRGLEALLGPTAAESLVQDGGDINLANSGQPAALL
                     LTDLVPGQYQPRTRMDEGALYELAESIKVQGIMQPILVRRLSDADAELKRSQLAPDTG
                     GALRPLYEIIAGERRFRAARLAGLDSVPVLVRDVPNESAAAMALIENIQREDLNPLEE
                     AQGLQRLIKDFGLTHDTAAQAVGRSRSATSNLLRLLNLAEPVQTMLMAGDVEMGHARA
                     LLALDKATQITAANQIAARKMSVREAESLVKKLGAEFSLVASKPKKEKSRDLKRVEEE
                     LSDLLTAEVEVRVKKRVKRAGRMEEAGELSIGFGSLDELNGLIDRLRSTAGR"
     gene            complement(3950..4489)
                     /locus_tag="PLB25_04805"
     CDS             complement(3950..4489)
                     /locus_tag="PLB25_04805"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE40936.1"
                     /translation="MNPVQILLLPGWQSSGPTHWQSLWCAQFGYQWVEQHDWMRPLRG
                     DWIARLEDVVLATEAPLLLVAHSMGCHLVAAWAAHSRHTGRVKGALLVAPGDAERDEL
                     RPILPSWCPPVLQALPFPGVLVASSNDPYCSFGRACQFASAWGADLFNAGALDHINAD
                     SALGDWPQGHGLLQAMIES"
     gene            complement(4547..5332)
                     /locus_tag="PLB25_04810"
     CDS             complement(4547..5332)
                     /locus_tag="PLB25_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOE40937.1"
                     /translation="MVKIFCIANQKGGVGKTTTTVNLAAGLAKVGQRVLMVDLDPQGN
                     ATMGSGIDKRKLELSVYDVLLESASVKEACVQSPKLQDAGCAYAILGANRELAGAEVE
                     MVSLERREKRLKTALTEVATDYDFILIDCPPSLSLLTLNGLCCAHGVIVPMQCEYFAL
                     EGLADLVNTIKQVHANLNKDLAIIGLLRVMFDPRITLQHQVSEQLKARFGDKVFNTVI
                     PRNVRLAEAPSYGLPGVVFDPASKGAQAFVAFAHEMVERIQKM"
     gene            complement(5565..6179)
                     /locus_tag="PLB25_04815"
     CDS             complement(5565..6179)
                     /locus_tag="PLB25_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOE40938.1"
                     /translation="MLGITDYGTFVITVIIFLAIPGPGNLALITSTSKGGIRGGIAAT
                     LGVILGDQVLMWSAVAGVAALLTAYPDAFNAVQWAGAAYLAWLGAQMLLAKPGAAPVL
                     NIKPHHFLKQALMITLLNPKAILFYMAFFPLFVDPVRQQGLITYGFMAATIAVLTFLY
                     GLIAVLLTYFLAERVRANPMVTRVLEKVAGLFLVGFGVKLALSR"
     gene            complement(6237..6926)
                     /gene="rsmG"
                     /locus_tag="PLB25_04820"
     CDS             complement(6237..6926)
                     /gene="rsmG"
                     /locus_tag="PLB25_04820"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HOE40939.1"
                     /translation="MSGLEHGLRQGLIELDLSLSDRQVTLLLAYLALIEKWTRVYNLT
                     AVRNPTEMLTHHLLDSLAAIKPLQQRLAATLATQGQHVGFPEATAIRLLDVGTGAGLP
                     GVVIAICCPEVNVHCVDTVGKKVAFIQQVVLALKLPNLRGIHARVESLTDRYQVVSSR
                     AFASLADFTAWSVAALAENGIWMAMKGKHPDDEIAALPAQVQVFHVEQLKVPGLTAQR
                     CIVWMRSARNP"
     gene            complement(6926..9055)
                     /gene="mnmG"
                     /locus_tag="PLB25_04825"
     CDS             complement(6926..9055)
                     /gene="mnmG"
                     /locus_tag="PLB25_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HOE40940.1"
                     /translation="MLYPQHFDVIVVGGGHAGTEAALASARMGCKTLLLTHNIETLGQ
                     MSCNPSIGGIGKGHLVKEVDALGGAMAAATDEGGVQFRILNSSKGPAVRATRAQADRV
                     LYKAAIRHRLENQPNLWLFQQAVDDLMVEGDRVVGAVTQVGIRFAGSTVVLTAGTFLD
                     GKVHVGLNNYPAGRAGDPPASSLSARLKELKLPQARLKTGTPPRIDGRSIDFSKCQEQ
                     AGDGMADQGVSAVPGGMPCGPAVPVFSFLGRPEQHPAQMSCWITHTNERTHDIIRSGF
                     DRSPMFTGKIEGIGPRYCPSVEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLP
                     FDIQYALVRSMAGLEDAHLLRPGYAIEYDYFDPQQLRSTFETRVISGLFFAGQINGTT
                     GYEEAAAQGLFAGINAALQVRAMGGGNGALSAAGSIDFGANAWLPARDEAYLGVLVDD
                     LINKGVTEPYRMFTSRAEFRLQLREDNADLRLTETGRKLGLVDDQRWAAFCRKRDAVS
                     RETERLRSIWISPKNLEAAESQRVLGKAIEHEYNLADLLRRPDVNYAALMSLAGGKFA
                     CPELPVHVPRETTGLSVDQALVLSVIEQVEISAKYAGYIDRQKADVGRAAHYENLKLP
                     QDLDYMQVAALSIEARQKLNRHKPETLGHASRISGITPATISLLMIHLKKTRFKGFAT
                     GVEAGSGLFPEATAELISDPIIELGAR"
     gene            9240..>9908
                     /locus_tag="PLB25_04830"
     CDS             9240..>9908
                     /locus_tag="PLB25_04830"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HOE40941.1"
                     /translation="MSTGKILVAQGGGPTAVINQSLVGVALEARRFGQIGHIYGALHG
                     VRGIINEDFVDLTQETSHNLEQVANTPSSALGSTRDKPDAAYCHEIFKVLQAHEIGHF
                     FYIGGNDSSDTVRIVSEEAQAANYPLRSIHIPKTIDNDLVGNDHTPGFPSAARFVAQA
                     FAGANLDNAALPGVYVCVVMGRHAGFLTAASALGKKFPADGPHLIYMPERVFVIDKFL
                     ADVKA"
     assembly_gap    9909..9918
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9919..10433
                     /locus_tag="PLB25_04835"
     CDS             <9919..10433
                     /locus_tag="PLB25_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010401663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HOE40942.1"
                     /translation="EGIHDAAGAPITAILAKELEHDAHGNVQLSGTGALADLLCEEIK
                     TKLKIKRVRGDTLGYMQRAFIGCVSDVDQREAREVGEKAVQFAMWGSKDGSVAIKRTG
                     FYSVDYELLPVSAVAGKTRTMEDEFITESGTDVTDAFRMYLRPLLGSGMPDAFRLRHN
                     PVAKVLNKKP"
BASE COUNT         2244 a         3315 c         3056 g         2145 t
ORIGIN      
        1 ctttcgtttg gtctgatgtg ccattcacgg ttccttgcgg atcaggaaat ctgcacttgg
       61 gtctggtagt cgcggcgctt gtcaaggtca tagacggcat cgagttggtc cagcttttcc
      121 agtgtgaatt cttcgctgcg gtcttcgccc agcaggccca ccgcatcggc gcgacactgg
      181 cggcagtgac gcatcagatt cgcgccgccc atgcaggcat cctgcaccac tttgagttct
      241 gcggcgcttg ggccgcgctg gccggtcagg ccaaacacgg tgccgtgttc gggctcggag
      301 atcagcggca tgatgttgtg caggaaagca ccacgcttct tgacttcgcg gttgacatcc
      361 atcaggtgct ggtcgttgat gccgggaatc agcactgagt tgatcttggt cagcacgcca
      421 cgggcggtga gcatttccag ccccagcatc tgacgctcgt gcagcacctt ggcggctgca
      481 taaccggtga ggcgcttgtg gttccagaaa atccaaggat agatcttggc accaattgcc
      541 gggtccacca tgttgatggt gatggtgaca tgatcgacct tgtacttgca gatttcgtcc
      601 accaggtccg gcaaggccag gccgttggtc gacaggcaca gcttgaggtc cggcgcctgc
      661 gcttgcagca tgcgaaaggt gtcgaaggtt tttttcgggt tggccagcgc gtcgcccggc
      721 ccggctattc ccagcaccgt catttgcggg atttcgctgg ccacggcgac caccttgcgc
      781 actgcctgct cgggcgtgag ccgttgcgac accacgccgg ggcgggattc gttggaacag
      841 tcgtatttgc ggttgcagta attgcactgg atgttgcagg ccggtgccac cgccacgtgc
      901 atgcgggcaa agtggtgatg tgcttcttcc gagtaacagg gatggtcctt gaccttgtcc
      961 cagatagcct ggggcatatc ggccgggccg tctgacgagc cacaactcga ctggccagcg
     1021 ccgacctgcg cggcgcagcc gctgccagca tcgggcagat cgagcctggc accgattgac
     1081 ttgatttgcc caatgctgac gtaggtcgtg cctggcatgg atgcggtgag cgcgcttgac
     1141 aacatggata cccttctggc gaccaacgtg atcgcatgca gggttattag ctaaatccgt
     1201 gccacctgaa agcagggttt tcaggcagcg ttttgtcgga tgtggcacag accacagacg
     1261 caaccggcac tgttttttga cgcagtgtca gtgatgcgac aaaggcaaca tctgacagat
     1321 caggcccacg catgcaacaa atcgcgcatg ttctgccagg caaacggagc cggttgccgc
     1381 cgcattcagt gggtttgcac tgttgaaagc cttgccggaa agcaaccagg ccggcatttc
     1441 tgccctgtcg gtgcggcagg tgatgcccag gtccactgtg gatcaccgct tgcaaaccat
     1501 gctgctggat tcctcaattg accgcttgtt tcccctctgt tagtccgcat gaatattgac
     1561 gtttttagct tcggcggggc tcgccctttg ggtcacagcg ctacgcttcc gatcaagcca
     1621 ctggcatcac gactggaggc gtagctcctt cctcgtccgc caaaacgaca tgctctgagc
     1681 aactcagcgt cgttgcccct ggcggcttgc tgcgacatgc agggcgcagc catgccacaa
     1741 ggcactgcct gcctcaagcc gtcaaaaggt gcgcaggcac gtttggagcc atagctttcg
     1801 ccctacccgg ccacgccgcc agcggcccac tcgggcatgc ccaactgagg aaaatccggg
     1861 atgattcaga aatgcaatag cgaccccagg tgcattgccg gtgcttcgat tgggcgcgtc
     1921 caactggaag catccaggcc aatgccagat tcaacagggc atctggtaca accacacagg
     1981 ccgaccatca ggcgcttgca acaccatctg agcctgcaac gctgtcgcct gaaactcaag
     2041 gctgatcagc caggcacccg gcttgagttc cgcacgcgct ttgttgccag ctcgcggcat
     2101 gctttccggg cgctgaaaca gatagaccag gtcgtaagca gaccagtcgg cgcgccagat
     2161 atcgccctgg cgcacccgcg cccaagggca gcgcaaggcg cacagcgcgc gcaacggcca
     2221 actccactcc agcccgtgca ggcgagcctg cggatacgcg ccacgcaagg ccttgaggcc
     2281 atcgcccaag ccgcaacctg catctaaaat aagagcatgc tgcgcaagtg gcacatgggc
     2341 tgcgagctct tttaatgcat tttttggggt ggggaacagt ggcgcgtcac gccaggcgtt
     2401 cagagggtag atcagcaaca gcagcagcaa ggaaagcaac caggaccagg ccggcagact
     2461 gaccgcgcca gaaaccgcca gcgacagtgg aaagcccagc gcaatcatca gcctgcgcca
     2521 ccaactagcg cccagcacgc tggccgccac gcccaggacg caggcaccag ccagcgcaac
     2581 caatatgcca agtcggcttt cggccagcgt gatgaacacc gcccaggcag cgagccagac
     2641 cagggcggca ggcaatggcc agggcagacg gtcaagcgtg aatctgctca cagtgtcagt
     2701 ttgcccaaga gccgtcgcca atctggccca gcgcagccgg tttggtcagc agctttaagg
     2761 actgagccgc atccggcaac tggtgcgaaa cacatttgaa atgaccaggt caaattggga
     2821 tgctgcgcca attctgcggc accaaaggcc acgcaaagac cccggtcaac cgattgggcc
     2881 acagcaagcc gatgcgccag acccagcact tcgccgctgc ggccgggtaa catgcgcggc
     2941 gacttcgggc tcaccgtccg gccgtgctgc gcagcctgtc aatcaggcca ttgagttcgt
     3001 cgagcgaccc aaagccgatg ctgagttcac ccgcctcttc catccggcca gcccgcttga
     3061 cgcgtttttt gacccgcact tccacctccg cagtaagcag gtctgacagt tcttcttcga
     3121 cgcgtttgag gtcacgcgac ttctcttttt tgggtttgct cgccaccaat gaaaactcgg
     3181 caccgagctt ttttaccaga ctctcggctt ctcgcaccga catcttcctg gcggcaatct
     3241 gattggctgc ggtgatctgg gtcgccttgt ccaaggccag caaggctcgc gcatggccca
     3301 tttcgacatc accggccatc aacatggttt gcaccggttc ggccaggttg agcaggcgta
     3361 gcaggttgct ggtggcactg cgggaacggc caaccgcctg cgcggcagta tcgtgggtga
     3421 ggccaaagtc cttgatcagg cgctgcagac cctgcgcttc ttccagcgga ttgaggtctt
     3481 cgcgctggat gttctcgatc agcgccatgg cggcggcgga ttcgttgggc acgtcacgca
     3541 ccagcaccgg cacgctgtcc agaccggcca gcctggccgc gcggaagcgc cgctcgccag
     3601 caatgatctc gtacagcggc cgcagcgcac cgccggtatc gggtgccaac tggctgcgtt
     3661 tgagttcggc atcggcatca ctcaaacgac gcaccagaat cggctgcatg atgccctgga
     3721 ccttgatcga ttcggccagt tcgtagagcg cgccttcgtc catgcgggta cggggctggt
     3781 attggcccgg caccaggtcg gtcagcaaca gcgctgcggg ctggccactg ttggccaggt
     3841 tgatgtcacc accgtcctgt accagtgatt cggccgccgt cggccccagc agtgcttcaa
     3901 gcccacgacc cagccctttt ggttttttgg tcaccatgtt tgtctttcgt taactttcaa
     3961 tcatcgcctg cagcaaacca tgcccttgtg gccagtcccc aagcgccgag tcggcattga
     4021 tatggtccag agcaccggcg ttgaacaggt cagcgcccca ggcactggca aactggcagg
     4081 cccgcccgaa actgcagtag gggtcgttgc tgctggccac cagcacacct ggaaaaggca
     4141 aggcctgcag gaccggcggg caccagctcg gcaaaatcgg gcgcaactcg tcgcgttcgg
     4201 catcgcctgg cgctaccagc aaagcgccct tgacccggcc agtgtggcgt gaatgcgccg
     4261 cccaggccgc caccagatgg cagcccatgc tgtgcgcaac cagcaacagc ggcgcctcag
     4321 tggccagcac cacgtcctcc aggcgagcaa tccagtcacc gcgcaatggg cgcatccagt
     4381 catgctgctc gacccactga tagccgaact gcgcgcacca caagctttgc cagtgggtcg
     4441 ggccagagct ttgccaaccc ggcaagagca ggatttggac tgggttcatt gtttcaattt
     4501 gaatagctgc tggcacagag tacatcgggg ctaccggcca ttttggctac attttttgaa
     4561 tgcgttcgac catttcgtgg gcaaacgcca caaaggcctg ggcacccttg ctggccgggt
     4621 caaacaccac acccggcaaa ccatagctgg gcgcctcggc cagacgcaca ttgcgtggaa
     4681 tcacagtgtt gaacaccttg tcgccaaagc gcgccttgag ttgttcactg acctgatgct
     4741 gcaaggtgat gcgcggatca aacatgacgc gcagcagacc gatgatggcc aggtccttgt
     4801 tcagattggc atgcacctgc ttgatggtgt tgaccagatc ggccaggcct tccagcgcaa
     4861 agtactcgca ctgcatcggc acaatgacac cgtgggcaca gcacaggccg ttgagtgtca
     4921 gcaaggacag gcttggcggg caatcaatca ggatgaaatc gtagtcggtg gccacttcgg
     4981 tcaaggcggt cttcaggcgc ttttcgcgcc gctccaggct gaccatttcg acttccgcac
     5041 cggcgagctc gcgattggca cccaggatgg cgtaagcgca gccagcatct tgcagcttgg
     5101 ggctttgcac gcaagcctct ttgactgagg ctgactcgag caagacgtca tagacgctga
     5161 gttcaagctt gcgcttgtca atgcccgagc ccatggtggc attgccctgc gggtccagat
     5221 cgaccatcag cacacgctgc cccaccttgg ccagcccggc cgcaagattg actgtggtgg
     5281 tggttttgcc aacaccaccc ttttggttgg ctatgcagaa tattttgacc ataaggtacc
     5341 gagcaaagag aaagggaatc taggggtctg tcggacttat ggaacatcgg ctgcgaatcg
     5401 atcgcagcgg tccagtttgc accggctttt tgacccatag cggggctatg ggcctgcaaa
     5461 tccgacgcaa actggcctcg ctggggccga ttctcgctgt tcgatccata agtccgacag
     5521 gctcctggcc aagatcattg tttcacgtga aacaggcact gaactcagcg cgacagcgcc
     5581 agcttgacac caaaaccgac caggaacaga ccagcgactt tttccagtac acgcgtcacc
     5641 atcggattgg cacgcacacg ctctgccaaa aagtaggtta gcaaaaccgc gatcaacccg
     5701 tacaggaatg tcagcacggc gatggtcgcg gccataaagc cataagtgat cagtccctgc
     5761 tggcgaaccg ggtccacaaa cagtgggaag aacgccatgt agaacaaaat tgccttggga
     5821 ttgagcaagg tgatcatcaa ggcctgtttc aagaaatggt gcggcttgat attgagtacc
     5881 ggtgcagccc ccggtttggc cagcaacatc tgcgctccca gccaggccag gtatgccgcg
     5941 cctgcccact gaacggcatt gaaagcatcc gggtaggcgg taagcaaggc agccacgcca
     6001 gccactgctg accacatcag aacctggtcc cccaggatca cgcccaaagt ggcagcaatg
     6061 ccaccccgaa tgccaccctt gctggtggag gtgatcaggg ccagattacc cggtccggga
     6121 atggccagga aaatgatgac agtgatgaca aaagtgccgt agtcggtgat gccgagcatg
     6181 aaggtctttc tgaaggtgta cccggaacgg tcgccgagcg tggtgccgag tttacgttag
     6241 gggttgcgcg cgctgcgcat ccacacgatg caacgctgtg ccgtcaaccc tggcaccttc
     6301 agttgttcca cgtgaaacac ctgcacctgg gcgggcagcg cggcgatctc atcgtccggg
     6361 tgtttgccct tcatggccat ccagatgcca ttttcggcca gggccgcgac cgaccaggcg
     6421 gtgaagtcag ccaatgacgc aaaggcccgc gaactgacca cctgatacct gtccgtcaga
     6481 ctctccaccc gcgcatggat gccgcgcagg ttcggcagct tcaatgccag taccacctgc
     6541 tggatgaaag ccaccttctt gccgacggta tcgacgcagt gcacattcac ctcaggacag
     6601 cagatggcaa tcaccacacc tggcaagcca gcaccggtac cgacatcaag cagtcggatt
     6661 gccgtcgcct cggggaagcc gacatgttga ccctgcgttg ccaaagtggc agcaagtcgc
     6721 tgctgcaaag gcttgatcgc cgccagactg tcaagcagat ggtgcgtcag catctcggtt
     6781 gggttccgca cggcggtcag gttatagacc cgggtccact tctcgatcaa ggccaggtaa
     6841 gccagcagca gcgtgacttg cctgtcgctc aagctcaggt caagctcaat caagccctgc
     6901 cgcaagccat gttccaaccc ggacatcagc gtgcgcccag ctctatgatt gggtcggaaa
     6961 tcagctctgc cgttgcctca ggaaacaaac cagagccggc ctcaacaccc gtagcaaaac
     7021 ccttgaatct ggtttttttc aggtgaatca tcagcaagga aatggtggca ggcgtgatgc
     7081 cggagatgcg cgacgcatga cccaaggttt caggcttgtg acggttgagc ttttggcgtg
     7141 cctctatgct caatgcggca acctgcatgt agtccaagtc ttgcgggagt ttgaggtttt
     7201 cgtaatgggc agcccttccg acatcggctt tttgccggtc gatgtagcct gcgtacttgg
     7261 ccgagatttc tacctgttca atcaccgaaa gtaccaacgc ctgatcgacc gacaggcccg
     7321 ttgtttcacg tggaacatgc actggcagtt cggggcaggc aaacttgcct cccgccaggg
     7381 acatcaatgc ggcatagttc acgtcggggc gacgcaacaa gtcggccagg ttgtattcat
     7441 gctcgatggc cttgcccagc acccgctgtg actccgctgc ttccagattc ttcgggctga
     7501 tccagatcga acgcaagcgc tctgtttcac gtgaaacagc gtcgcgcttg cgacaaaacg
     7561 ccgcccagcg ctgatcgtcc accaaaccca gcttgcggcc ggtttctgtc aggcgcaggt
     7621 cggcattgtc ttcacgcaac tgcagacgaa actcagcccg actggtgaac atgcgatatg
     7681 gctcggtgac gcccttgttg atcaggtcat ccaccagcac accaagatat gcctcgtcac
     7741 gcgcaggtag ccacgcattc gcaccgaagt caatagagcc ggcagcactg agcgcgccgt
     7801 tgccaccgcc catggcgcgc acctgcagcg cggcattgat gccagcaaac aaaccttgcg
     7861 ccgcagcctc ttcatagccg gtagtgccgt tgatctgacc tgcaaaaaac aggcctgaaa
     7921 tcacgcgggt ttcaaacgtg cttctgagct gctgcgggtc aaaataatcg tactctatgg
     7981 catagccagg ccgaagcaaa tgcgcgtctt ccagcccggc catgctgcgc accagcgcat
     8041 actgaatgtc aaacggcagg ctggtactga tgccattggg gtagtactcg tgggttgtca
     8101 ggccttcagg ctccagaaag atctggtggc tgtccttgtc ggcaaaccgg ttgatcttgt
     8161 cttcgacact cgggcaatag cgcggcccga tgccttcgat cttgccggtg aacatcgggc
     8221 tgcggtcaaa gccgctgcgg atgatgtcat gcgtgcgctc gttggtgtgg gtgatccagc
     8281 acgacatctg cgccgggtgc tgctcgggtc ggcccaaaaa actgaatacc ggcacagccg
     8341 gaccgcaagg catcccgcct ggaactgcac tcaccccttg atctgccatg ccgtcaccgg
     8401 cctgctcctg gcatttgctg aaatcaattg aacggccatc aatgcgcgga ggggtgccgg
     8461 ttttcaggcg cgcctgtggc aacttcagct ccttcagccg cgcgctcaaa ctcgaggctg
     8521 gcggatcacc ggcacgccca gcggggtaat tgttcagccc cacatgcact ttgccgtcca
     8581 aaaaagtgcc tgctgtgagc accaccgtgc tgccagcaaa ccggataccg acctgcgtca
     8641 ccgcacccac cactctgtcg ccttcaacca tcaggtcatc taccgcctgt tgaaataacc
     8701 acagattggg ctggttttcc agacgatgtc gaatcgcggc cttgtacaaa acacggtcag
     8761 cctgcgcgcg cgtggcacgc actgccggcc ccttgcttga gttgaggatg cgaaactgca
     8821 caccaccctc atccgtggca gctgccatgg caccccccag cgcatcgact tccttgacca
     8881 aatgcccttt gccaatgccg ccaattgatg ggttgcaact catctggccc aatgtctcga
     8941 tgttgtgcgt gagcaacaag gtcttgcaac ccatgcgcgc agacgccagc gcagcctcgg
     9001 tgccggcatg gccgccgccg acgacgatca cgtcgaaatg ttggggataa agcatggtgg
     9061 ttgatcgaat tgggacgata cagaaaatgg cgctcgccct gcgcttgaac gacaagtggc
     9121 gattttaccg aaggccccta aaacatcacc caccacaaga catcaaccca gcaaaacggt
     9181 cacttcacag gcacaatgcg tgcttgcgat ttcactacac ccttacaaca ggagaacaca
     9241 tgtcaaccgg aaaaattctc gtcgcacaag gcggcggccc caccgctgtc atcaaccagt
     9301 ccctggtggg cgttgcgctc gaagcgcgcc gcttcggcca aattggtcac atctacggtg
     9361 cactgcacgg tgtgcggggc atcatcaacg aggactttgt cgatctgacc caggaaacca
     9421 gtcataacct ggaacaagtc gccaacacgc cttcctccgc gctcggatct acgcgcgaca
     9481 agccggacgc agcttattgc cacgagattt tcaaggtgct gcaagcccac gaaattggcc
     9541 atttctttta catcggtggc aatgactcca gcgacacggt acgcatcgtc agcgaggaag
     9601 ctcaagcagc caactacccg ctgcgctcga tccatatccc gaaaaccatc gacaacgatc
     9661 tggttggcaa tgaccacacg cccggtttcc cgtctgcagc tcgttttgtg gcgcaggcat
     9721 tcgccggcgc caacctcgac aatgctgccc tgccaggtgt ctatgtgtgt gtcgtgatgg
     9781 gtcgccacgc tggctttttg accgcagcgt ccgcccttgg caagaaattt ccggcggacg
     9841 gcccgcacct gatctacatg cccgagcgtg tttttgtcat agataaattt ctcgctgatg
     9901 tcaaggccnn nnnnnnnnca gagggcattc atgatgccgc aggcgcgccc attactgcca
     9961 tattggccaa ggaactggaa cacgatgccc acggcaacgt gcagctctct ggcactgggg
    10021 cgctagccga tttgctgtgt gaagagatca agaccaagct caaaatcaag cgtgtgcggg
    10081 gcgacacctt aggctatatg cagcgcgcat tcattggctg cgtgtcggat gtagatcagc
    10141 gcgaagcccg cgaggtcggt gaaaaggccg tgcaatttgc catgtggggc tccaaggatg
    10201 gctcggtcgc catcaagcgc accggttttt actcggtcga ttacgagttg ctgccggtct
    10261 cagccgtagc cggcaaaacc cgcaccatgg aagatgagtt catcaccgaa agtggcaccg
    10321 acgtgactga tgccttccgc atgtacctgc gccctcttct gggctctggc atgcccgatg
    10381 ctttccgtct gcgccacaac ccggtggcca aagtgctgaa caagaaaccc tgaaccgggc
    10441 caacgggttc gcggtctgca gcgcccacta cctgcagacc gtgccccatt cagttgttat
    10501 gggctgccgc tagctgcgct tgtaggttac ggtgagccgc accgtatagc tattatatag
    10561 atagctagca gcgcatgcca gataacggct taaaggcgat ttggctggta taacctgatt
    10621 cagccggtgc tttgggcaag ccgtttaaac tcagcagtct gtttgtcgtg actggcccgc
    10681 aatgggcccc ggttgcgcaa ctgaccccac cattgcacta acaatcaaat gaagctttat
    10741 tactcacctg gcgcctgctc actggcaccc
//
LOCUS       DAOSLL010000074        10729 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6042_length_10729_cov_5.017613, whole genome shotgun sequence.
ACCESSION   DAOSLL010000074 DAOSLL010000000
VERSION     DAOSLL010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10729)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10729)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10729
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6042_length_10729_cov_5.017613"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="PLB25_04840"
     CDS             <1..293
                     /locus_tag="PLB25_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HOE40943.1"
                     /translation="ILCNARAMGEFGAVSVVSGHIRGETNTLPLHVEILYNEYQSVAA
                     FAVASLLTLLALLTLLIKSVVEWQFERDQKAAASLPPERPSADALPTARTAG"
     gene            307..1404
                     /locus_tag="PLB25_04845"
     CDS             307..1404
                     /locus_tag="PLB25_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HOE40944.1"
                     /translation="MSIEIRNISKDFGSFQALRNVSLTIESGELVALLGPSGCGKTTL
                     LRIIAGLEAADQGQILFNGLDTTDKATRERHVGFVFQHYALFRHMSVFDNVAFGLRMK
                     PRASRPAASVIRQKVHDLLGLVQLDWLADRYPSQLSGGQRQRIALARALAVEPQVLLL
                     DEPFGALDAKVRKELRRWLRRLHDDLHVTSIFVTHDQEEALEVADRVVLMNSGQVEQI
                     GSPQQVSDHPASPFVYGFLGDVNRFHGRVHQGEMRLGKDPHGLRLAAPEHRAVQDSQA
                     VAYVRPHHLEVQRYLAGADAADQPGGILARLTRAMVVGPIARLELSALDSSSSNDLIE
                     AQIPAREFRELALSEGETVLLMPYQARVFLA"
     gene            1457..1639
                     /locus_tag="PLB25_04850"
                     /pseudo
     CDS             1457..1639
                     /locus_tag="PLB25_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328818.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1909..2352)
                     /locus_tag="PLB25_04855"
     CDS             complement(1909..2352)
                     /locus_tag="PLB25_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40945.1"
                     /translation="MDNANNAPILSSLTIAGYRSFGAEPEFFPKFSKINILIGQNNCG
                     KSNVLRFIHDWMAKLQQHPRPTLTHHDRHLPTGGIGLLYGPLPPYICNGQIDFEAISA
                     HSKINSGVRKQSTLSTLSGCSRKHQRQTTNPLVRGSTFQEMAKFR"
     gene            complement(2410..3771)
                     /locus_tag="PLB25_04860"
     CDS             complement(2410..3771)
                     /locus_tag="PLB25_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006464994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOE40946.1"
                     /translation="MTTDTTVLHAIQSTPTGLTLAELLKQHPSLARRTVQRWVSQRIS
                     AGQITAYGKGRARRYLPIAAATDTPPTPSGSFPTQLTLSADSRDILAYVTQPLQARKP
                     VGYQRDFLDAYQPNISAYLSTPLRRQLHNMGKTSRMSEPAGTYSRAILNRLLIDLSWA
                     SSNLEGNTYSRLDTVALIEHGRTATGKGAIETQMILNHKVAIELLVDNAGTAELNRYL
                     LMNLHSALAENLLPNPADEGRVRQHAVDIGKSVYRPLSTLHQIDETLDLVLAKLNQIN
                     DPFEQSFFAMVHLPYLQPFADINKRSSRLAANLPLFRANLCPLTFLDVPETAYSLATL
                     GVYELTRVELLRDLYVWAYERSTQEYQAIQQDLAEPDPLRLAYRDLIKQTVREVVQQP
                     QADPLKVIDRALKPIPLAEQTDLYALLVQELQRLHEGVLARYGLRPSELAAWKSAQYL
                     LPL"
     gene            complement(3906..4184)
                     /locus_tag="PLB25_04865"
                     /pseudo
     CDS             complement(3906..4184)
                     /locus_tag="PLB25_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802511.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
     gene            4359..4544
                     /locus_tag="PLB25_04870"
     CDS             4359..4544
                     /locus_tag="PLB25_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40947.1"
                     /translation="MSLNNLANLQSSHGDAPAQAEALTCARESVGIYRALAKDQPEAC
                     ERYLKIAQRVLASVESL"
     assembly_gap    4644..4743
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(4871..5374)
                     /locus_tag="PLB25_04875"
     CDS             complement(4871..5374)
                     /locus_tag="PLB25_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOE40948.1"
                     /translation="MNPIPATDRFQALDACHQQIHLHLEKLASVLVQIEAYEDESQYR
                     LQAREIEAFFSGTSRQHHADEEKNIFPGLLASDNAELVHAVRTLQQDHGWIEQNWVEL
                     APMLRAIAEGEDWVDMAELQHTVEVFLSLCHDHIVLEETLIYPESKRQFSAELAARNK
                     RIAEQAK"
     gene            5597..7444
                     /locus_tag="PLB25_04880"
     CDS             5597..7444
                     /locus_tag="PLB25_04880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE40949.1"
                     /translation="MQATNTANPAYFHKVVDCQWACPAHTPVPEYIRLIGQGRYSDAY
                     MINWVSNVFPGILGRTCDRPCEPACRRSRVEENNGSKPEPVAICRLKRVAADFKDDVK
                     SRMPAIAPFNGKRIACVGAGPASLTVARDLAPLGYEVTVFDGEAKAGGFIRSQIPRFR
                     LPESVIDEETGYILDMGVKFNNRQRVESMQALLSQDYDAVFVGCGAPRGRDLEVPGRA
                     EAAANIHIGIDWLASVSFGHITSIGERVIVLGGGNTAMDCCRSARRLGGKDVKVIVRS
                     GFEEMKASPWEKEDAQHEGIPILNFHVPKTFEHADGKLTGMTFEIVRAEYDAKGRRSL
                     IPTGEPDVLVACDTVLVAVGQENSFPWIERDCGIAFDKWGLPVLGEGTFQSTIPKVFF
                     GGDAAYGPKNIITAVAHGHEAAVSIDRLLNGEDVARRPAPMTNLMSQKMGIHEWIYRN
                     DVSNDVRFAVPWAKAETALASIKVEVELGFDAATAFKEASRCLNCDVQTVFNRGTCIE
                     CDACVDICPMDCITFTANGDEADLRTRLKAPAGNQTQDLYVSAELKTGRIMVKDEDVC
                     LHCGLCAERCPTGAWDMQKYLLNTTQAGPRSRDAKPAAAPALYAHPEAA"
     gene            7441..9255
                     /locus_tag="PLB25_04885"
     CDS             7441..9255
                     /locus_tag="PLB25_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOE40950.1"
                     /translation="MKRIEAINDFVIKFANVNGSGSASANELFAKAVMRMGVPVSPRN
                     IFPSNIQGMPTWYEARVCEKGYQGRRGGVDMMVAMNPQTWDADVAEIETGGYLFYDST
                     RPMPAAKFRDDVQNMGVPLTEIANAAYADPRQRQLFKNIIYVGALSVLLDVDAEVFEK
                     LFAEQYKGKERLLESNVKALHLGRDHVKQHLQAPLGIRVQRADNVGDQIFTDGNSAAG
                     LGLVYGGATVAAWYPITPSTSIPEAFQKYCEKFRVDPITGQNKFAIVQAEDELASIGM
                     VIGAGWNGARAFTATSGPGVSLMTEFIGLAYFAEIPVTIINVQRGGPSTGMPTRTQQS
                     DLLSCAYASHGDTKHVLLFPQDPHECFTHAATALDLAERLQTPIFVMTDLDIGMNQRL
                     CKPFVWDDARQYDRGKVMSAAELEAGKPFGRYRDVDGDGIGWRTLPGTHPSKGAYFTR
                     GTTRNPQAVYSEAGPDYIYNMERLLRKFIVAATLVPQPIFRPADKPTRLGVIYYGSTT
                     PAMHEALDVLERNGIAVDAMRLCAFPFPDSVSEFIAAHDKVFVAEQNRDGQMRSMLIN
                     ELEINPAKLVRVLHFDGTPITARFIIRTIHDNVQKELA"
     gene            9252..10307
                     /locus_tag="PLB25_04890"
     CDS             9252..10307
                     /locus_tag="PLB25_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015865533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HOE40951.1"
                     /translation="MTFIAKPRLHHPTLHTNRVGFTRRDYEGRISTLCAGCGHDSISA
                     AIIQACWELDIEPHRVAKLSGIGCSSKTPDYFLGASHGFNTVHGRMPSVLTGANMANR
                     ELLYLGISGDGDSASIGLGQFANAMRRGVRMAYIVENNGVYGLTKGQFSATADRGSKS
                     KKGVINSDSPVDLVGIALQLGATYVARSFSGDKAQLVPLIKGAMAHGGAAFIDVISPC
                     VTFNNHGGSTKSYDYVRQHNEAVSRIDFITPGSEITTEYGPGELVEVEQHDGSSLRLR
                     KLHTDYDPTDRYAAMSYMHSHAALGEIVTGLLYIDPLATDLHTSMNTIAGPLNTLGAA
                     QLNPGQKALDKINAALR"
     gene            10356..>10729
                     /locus_tag="PLB25_04895"
     CDS             10356..>10729
                     /locus_tag="PLB25_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOE40952.1"
                     /translation="MPERTVFQSMVQRHVIGVLPTATVHTAACVMTRNNSSSVLVIDA
                     ANTMQGIITERDMITRVLAKALDPSQTPVAEVMTRSPHCVFPDTKVADAVLMMIERGF
                     RHLPVVTADSHKILGVFSVRDA"
BASE COUNT         2145 a         3148 c         3180 g         2156 t
ORIGIN      
        1 tgattttgtg caacgcccgc gccatgggcg agtttggcgc ggtgtcggtg gtgtccggcc
       61 atattcgcgg cgagaccaac acattgccac tgcatgttga aattctctac aacgaatacc
      121 agtcggtggc ggcttttgcc gtggcctctt tgctgacgct gctggcgttg ctcactttac
      181 tcatcaagtc ggtggttgag tggcagtttg agcgggacca aaaagctgca gcaagcctgc
      241 cgccagagcg cccatccgca gacgcgctgc cgacagcaag aaccgccgga tgacagaaaa
      301 cacaccatga gcattgaaat ccgcaacatc agcaaagact ttggcagctt ccaggcgctg
      361 cgcaatgtgt cactgaccat cgaatcaggc gagctggtgg cgctgctggg gccgtcgggc
      421 tgcggcaaaa ccacgctgtt gcgcatcatc gccgggctgg aggctgcgga ccagggtcag
      481 atcctgttca acggtctcga caccaccgac aaagccacgc gcgagcgcca tgtcggtttt
      541 gtctttcagc actacgcgct ctttcgtcac atgagcgtgt ttgacaacgt ggcctttggc
      601 ctgcgcatga aaccccgggc aagtcgcccg gccgcatcag tgatcaggca aaaggtgcac
      661 gatttgctgg gcttggtgca actcgactgg ctggcggacc gctatccgtc gcaactctcg
      721 ggtggccagc gtcaacgtat tgccctggcc cgggccctgg cggtggagcc acaggtgctg
      781 ctgctcgatg agccatttgg cgcactcgat gccaaagtgc gcaaggaact gcgccgctgg
      841 ttgcgccgcc tgcacgacga tttgcatgtg accagcatct ttgtcaccca cgaccaggaa
      901 gaagcgctgg aagtggccga ccgggtggtg ctgatgaaca gcggccaagt cgagcagatc
      961 ggctcgccgc aacaggtctc ggatcacccg gccagtccgt ttgtctatgg ctttctgggc
     1021 gatgtgaacc ggtttcacgg ccgcgtgcac cagggcgaaa tgcgccttgg caaggacccg
     1081 catggcctgc gtctggccgc acccgaacac cgcgcggtgc aagacagcca ggcggttgct
     1141 tatgtgcgcc cgcatcatct ggaggtgcaa cgctacctgg caggtgccga cgcagccgat
     1201 cagcccggcg gcattctggc cagactgacc cgcgccatgg tggtcgggcc aatcgcccgg
     1261 ctcgaactgt cagcgctcga cagcagcagc agcaacgacc tcatcgaggc gcaaataccc
     1321 gcccgcgagt tccgcgagct ggccctcagc gaaggcgaaa cggtgctgct gatgccgtac
     1381 caagcgcgcg tgttcctggc ctgaagtgaa gaacgaaata gcccgccaga cagcgcgcaa
     1441 tatgcgctga cagctagtgt caccgccggg aatcgtcttt cacccggcca ccggcattga
     1501 tgcggatgcc ctggctcagg tgcaagccac cttgagcaga cgcatcctgc gcgccttcgt
     1561 gggccggagc ctgatcgagc aagcagatgc caaagagatg ctggcctacc agcacagcgg
     1621 gtcttcggtg gacgctggcg ccctgattgc cacccaattt cccccacccg gttgtgggac
     1681 cgaaacgcac tcaaaggtcg cgtctcacgc cgctggtttg tgctcgctac gcgaggggaa
     1741 ccgttatcaa gaaggggcca tggcaacgac ttggaaaaga actttttcgc catcacacct
     1801 gggggaattt ggggtggcca caggtggggg tatttcaagt ggccttcagg aaccacaatt
     1861 cttgaagctc gttgtcacca attcttttga tcgactgatc ccaactgttt acctgaactt
     1921 cgccatttct tgaaaagtgg aaccacggac caacgggttt gttgtctgtc tttgatgctt
     1981 cctcgaacag ccgcttaaag tgctcaatgt gctttgtttt cgaactccgc tgttgatctt
     2041 ggaatgcgcg gaaatcgcct cgaaatcaat ttgtccatta caaatgtacg gaggtagcgg
     2101 tccatacagc agcccgattc ctccagtcgg caagtgcctg tcatggtggg ttaaggtcgg
     2161 acgcggatgc tgttgaagtt tggccatcca atcatggata aaccgcaaca cattggattt
     2221 gccgcagttg ttctggccaa tgaggatatt gatttttgag aatttcggga aaaactctgg
     2281 ttccgcacca aaactgcggt aacccgcgat ggtgagtgag ctgaggatcg gtgcgttatt
     2341 ggcgttgtcc atggtgcccc atcttttggt tgatgcctct agccttttaa cgtaaagcgt
     2401 caatagctcc taaagaggta gcaaatactg cgcacttttc catgccgcca actccgaagg
     2461 ccgcagtcca taccgcgcca acaccccctc atgcaagcgc tgtagttctt gcaccaataa
     2521 cgcgtacaga tccgtctgtt ctgccagggg tatcggcttc agggcgcggt caatcacctt
     2581 gagcgggtcg gcctgcggct gttgcaccac ctcccgcacc gtctgcttga tcaaatcgcg
     2641 gtaggccagg cgcagcgggt cgggttctgc aaggtcttgc tggatggcct ggtattcctg
     2701 ggtggagcgc tcgtaggccc agacgtacag gtcgcgcagc agttccaccc gtgtcagttc
     2761 gtaaaccccc aaggtggcaa ggctataagc cgtttccggc acatcaagga aagtcagcgg
     2821 gcacaggttg gcgcgaaaca agggcaggtt ggccgccagg cgtgagctgc gtttgttgat
     2881 gtcggcaaat ggttgcaggt agggcaggtg caccatggca aagaaggact gctcaaacgg
     2941 gtcgttaatc tggttcagct tggccagcac caggtccagt gtttcgtcaa tctggtggag
     3001 cgtggacaac gggcggtaca cgctcttgcc aatgtccacg gcatgttggc ggacacggcc
     3061 ttcatcggcg gggttgggca gcaggttttc cgccagggcg ctgtgcaggt tcatcagcag
     3121 gtagcggtta agttcagcag ttcctgcatt gtccaccagc agttcaatgg ccaccttgtg
     3181 gttcaggatc atctgtgttt cgatcgcgcc cttgccggtg gcggtgcgcc cgtgctcgat
     3241 cagtgccacg gtgtcaaggc gcgaataggt gttgccctcc aggttgctgg atgcccacga
     3301 caggtcaatc agcaggcggt tcaggatggc gcggctgtaa gtgccggcag gctcactcat
     3361 gcggctggtt ttgcccatgt tgtgcaactg gcgacgcagc ggtgtgctca ggtaggcact
     3421 gatgttgggc tggtaggcat ccagaaagtc gcgctggtag cccaccggct tgcgcgcttg
     3481 caagggctgg gtcacgtagg ccaggatgtc ccggctgtct gccgacaacg ttaactgggt
     3541 cggaaaactg ccggacgggg ttggtggcgt atccgtagca gcagctatcg gcaggtagcg
     3601 ccgtgcgcgg cccttgccat aggcggtaat ctggccggcc gaaatccgtt ggcttaccca
     3661 gcgctgtacc gtgcgccgtg ccaacgatgg gtgttgtttc agcagttcag ccagtgttaa
     3721 gccggttggt gtggactgaa tggcgtggag cacggttgtg tcggtcgtca tgatggtttg
     3781 tggcgcagtt tcattggact tggtggtaat tgtggcgcag tttgaagaac tgcgccaatt
     3841 ggtgctgtac accgttgggt cacaaaaaca ccatggcgtt tttgctttca gggcagaagc
     3901 agctttcagc gattgcccgc aggtgatgct tgtcggcctt tcacccccgc cccccgcccc
     3961 tccagtctgg tctcatcgag caccttgccc cgcattccca caatctgcac cacatgtcgg
     4021 ccaggccagg gccgccactg tgcggtcgcg cctttggcaa agggtttgcc atcaagcagc
     4081 cagcgcagct tggcaccctc ggcggcaaaa ctcaaccgct gccgattcgg tggaatgtcc
     4141 gggtccaggg cgatgatggt gccactggtg ggggcggttg cgcgtgaatg gatatccttg
     4201 gtgatcgttc tttcagcaga ggaaaatttt cctgaatccg ttaggtatat tgcctatggc
     4261 cctcgtggaa tatacgtagt taactcctga aaaaatagcg aaagtggttt acttcatgcc
     4321 gcccaaaaac aacgccaggc gcgtagcctc atctggccat gtcgctcaac aacctggcca
     4381 atcttcagtc gagccacggt gacgccccgg cgcaagccga agcactgaca tgcgcgcgcg
     4441 agtcggtggg catttaccgc gcactggcta aagaccagcc agaagcatgc gagcgctact
     4501 tgaaaatagc gcaacgggtt ctggcaagcg tggagtcatt gtgagggctg aacaactgca
     4561 atgacgtgac atcgggttga cttacgaaac gtcgcaacgg gattctgagt ctcgactagg
     4621 ttcaacggtg tgctgcagtt cggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnccatatc gacccaatct tcaccctcgg caatggcgcg cagcatgggg gcaagttcgg
     4801 gcctgaagtg aagaacgaaa tagcccgcca gcgcccgtgc aatatgcgct gacagctatc
     4861 aaataagtag ctatttggcc tgctctgcga tccgcttgtt gcgcgctgcg agttccgctg
     4921 aaaactgcct cttggactct ggataaatca gtgtttcttc cagcacgatg tggtcgtggc
     4981 acagcgacag gaagacttcc acggtgtgct gcagttcggc catatcgacc caatcttcac
     5041 cctcggcaat ggcgcgcagc atgggggcaa gttccaccca gttttgctcg atccagccgt
     5101 ggtcctgctg cagcgtgcgc acggcgtgga ccagctcggc gttgtcgctg gcgagcaagc
     5161 cagggaaaat attcttctct tcgtcggcat ggtgctggcg cgaggtgccc gaaaaaaaag
     5221 cttcaatctc gcgggcctgc aggcgatatt gcgattcatc ttcgtaggct tcaatttgta
     5281 ccagcacact ggccaacttt tccaggtgca ggtgaatttg ctggtggcag gcatcaagcg
     5341 cctggaatcg gtcagttgcg gggatcgggt tcatggggca ctcctaaaga tggttggatt
     5401 cggcaattgg ggcaatgcta ccgtattgcc attggcgatc agacaaggca acttttcaat
     5461 ccggaccggt acttgatgaa aatcaacaaa tttgcagatt ttgaaaaaac tcgcccggac
     5521 acactcaatc tatgagcgta ccatcgcgca accagcggca gtacgagcga ctacccggca
     5581 gaataaggag accaagttgc aagccaccaa caccgccaac ccggcctatt ttcacaaggt
     5641 cgttgactgc cagtgggcct gtccggccca tacgcctgtg cccgagtaca tccgcctgat
     5701 cggtcagggt cgctacagcg atgcctacat gatcaactgg gtgtccaacg tgtttcccgg
     5761 cattctcggg cgcacttgcg accggccctg tgaaccggcc tgccgacgca gccgggtgga
     5821 agaaaacaac ggcagcaagc ctgaaccggt ggcgatttgc cgcctcaagc gggttgccgc
     5881 tgacttcaag gacgacgtga agtcgcgcat gccggccatc gcaccgttca acggcaagcg
     5941 cattgcctgc gtcggagccg gaccggcatc gctgacggtg gcgcgcgacc tggcaccgct
     6001 gggctacgag gtgacggtat ttgacggcga ggccaaggcg ggcggattta tccggtcaca
     6061 aatcccgcgc tttcggctgc ccgagtcggt gattgacgag gaaaccggct acatcctcga
     6121 catgggtgtc aagttcaaca accggcagcg ggtcgagtcg atgcaggcgc tgctctcgca
     6181 agactatgac gcggtgtttg tcggctgcgg cgctccgcgc gggcgtgacc tggaggtgcc
     6241 gggccgcgcc gaggcggcgg ccaacatcca tatcggcatc gactggctgg cctcggtctc
     6301 gtttggccac atcaccagca tcggcgaacg tgtcattgtg ctcggtggcg gcaatacggc
     6361 gatggattgc tgccgctcgg cgcgccgtct gggtggcaag gatgtgaagg tgattgtgcg
     6421 cagtggcttc gaggaaatga aagcctcgcc ctgggaaaaa gaagacgcgc agcatgaagg
     6481 cattccgatc ctgaactttc atgtgcccaa gactttcgaa cacgctgatg gcaagttgac
     6541 cggcatgacc ttcgagatcg tgcgcgccga atacgatgcc aaaggtcggc gcagcctgat
     6601 tccgaccggt gagcccgatg tgctggtggc ctgcgacacg gtgctggtgg ctgtcgggca
     6661 ggaaaattcg ttcccgtgga tcgagcgcga ctgcggcatt gcgtttgaca agtggggcct
     6721 gccagtgttg ggtgagggca ccttccaatc gaccataccc aaggtatttt ttggcggtga
     6781 tgccgcctat ggcccgaaaa acatcatcac cgctgtggcc catggccatg aagcggcggt
     6841 gtcgattgac cggctgctca acggcgaaga tgtggcgcgc cgcccggccc cgatgaccaa
     6901 tttgatgtca caaaaaatgg gcatccacga gtggatctac cgcaacgatg tttccaacga
     6961 tgtgcgtttt gctgtgccct gggccaaggc cgaaaccgcg ctggccagca tcaaggttga
     7021 agtggagctg ggttttgacg cagcaaccgc cttcaaggaa gccagtcgct gcctcaattg
     7081 cgacgtgcag acggtgttta accggggcac ttgcattgag tgtgatgcct gcgtcgatat
     7141 ttgcccgatg gactgcatca ccttcactgc caacggcgac gaggccgatt tgcgcacccg
     7201 gctgaaagcc ccggcaggca accagacgca agacctgtat gtgtcagccg agctcaagac
     7261 cggccgcatc atggtcaaag atgaagatgt ctgcctgcac tgcggcctgt gtgccgagcg
     7321 ttgcccgacc ggcgcctggg acatgcagaa atacctgctc aacacgacgc aggccgggcc
     7381 ccgcagccgc gatgccaagc ccgctgccgc gccagccctt tacgcccacc cggaggcagc
     7441 atgaagcgca ttgaagccat caacgatttc gtcatcaaat ttgccaacgt caacggctct
     7501 ggttcggcct ctgccaatga gttgtttgcc aaggccgtga tgcgcatggg cgtaccggtg
     7561 agcccgcgca acatctttcc gagcaacatc cagggcatgc cgacctggta cgaagcccgc
     7621 gtctgtgaaa aaggctacca ggggcgtcgc ggtggtgtgg acatgatggt ggcaatgaac
     7681 ccgcagacct gggatgccga tgtggccgaa atcgaaaccg gcggctacct gttttacgac
     7741 agcacccgac ccatgcctgc cgccaagttc cgcgacgacg tgcagaacat gggtgtgccg
     7801 ctgaccgaga ttgccaacgc cgcttatgct gacccgcgcc agcgccagtt gttcaagaac
     7861 atcatctatg tgggtgccct gtcggtgctg ctcgatgtcg atgccgaggt gttcgagaaa
     7921 ctctttgccg aacagtacaa gggcaaggag cgcttgctgg agtccaatgt gaaggccctg
     7981 cacctgggcc gcgaccatgt caagcaacac ttgcaagcgc cgctgggcat tcgggtgcag
     8041 cgggccgaca acgtgggtga ccagatcttt accgacggca acagcgctgc cgggctcggg
     8101 ctggtgtatg gcggtgccac ggtggctgcc tggtatccga tcacgccgtc cacgtcgatt
     8161 cccgaggctt tccagaaata ctgcgagaag ttccgcgttg acccgatcac cggccagaac
     8221 aagtttgcca tcgtgcaggc cgaagacgaa ctggccagca ttggcatggt gattggtgct
     8281 ggctggaacg gtgcccgtgc ctttaccgcc acctccgggc ccggcgtttc gctgatgacc
     8341 gagttcattg gcctggccta ctttgccgag attccggtca ccatcatcaa tgtgcagcgc
     8401 ggcggaccca gcaccggcat gccgacccgc acgcaacagt ctgacctgct gagttgtgcc
     8461 tatgcgtcgc acggcgacac caaacatgtg ctgctgttcc cgcaggaccc gcacgagtgc
     8521 tttacccacg cggccaccgc gctggacctg gccgagcgcc tgcaaacgcc gatttttgtc
     8581 atgaccgacc tcgacatcgg catgaaccag cggctgtgca aaccgtttgt ctgggacgat
     8641 gccaggcagt acgaccgtgg caaggtgatg agcgccgcag agctcgaagc cggcaaaccg
     8701 tttggccgct accgcgatgt cgatggcgac ggcattggct ggcgcaccct gcctggcacg
     8761 caccccagca agggcgctta ctttacccgt ggcaccacgc gcaacccgca ggcggtctat
     8821 tccgaagccg ggccggacta catctacaac atggagcgcc tgctgcgcaa gttcatcgtt
     8881 gcagccacgc tggtgccgca gccgattttc cgcccggcag acaagccaac ccgcctcgga
     8941 gtgatctatt acggctccac cacaccggca atgcatgagg cacttgatgt gcttgagcgc
     9001 aacggcattg cagtggatgc catgcgcctg tgcgcgtttc cgttccccga ttcggtgtcg
     9061 gaattcattg ccgcgcacga caaggtattt gtggccgaac aaaaccgcga cggccagatg
     9121 cgctccatgc tcatcaatga actggagatc aacccggcca aactggtgcg tgtgctgcat
     9181 tttgacggca cgccgatcac cgcccgtttc atcatccgca ccattcacga caacgtgcaa
     9241 aaggaactcg catgaccttt attgccaaac cccggctgca ccacccaacc ctgcacacca
     9301 accgggtcgg cttcacccgg cgcgactacg aggggcgtat ttcgaccctg tgcgctggct
     9361 gtggtcacga ttcgatttct gccgccatca ttcaagcctg ctgggagctc gacatcgagc
     9421 cgcaccgcgt ggccaagctg tccggcattg gctgtagctc aaaaacaccc gactattttc
     9481 ttggtgcctc acacggcttc aacaccgtgc acgggcgcat gcccagcgtg ctgaccggtg
     9541 ccaacatggc caaccgcgag ctgctgtacc tgggcatctc gggtgacggt gactcggcat
     9601 cgattggcct gggccaattt gccaatgcca tgcggcgtgg cgtgcgcatg gcctacatcg
     9661 ttgaaaacaa cggcgtttat ggcctgacca aaggccagtt ctcggccacc gcagaccgcg
     9721 gctcaaaaag caaaaaaggc gtgatcaaca gcgacagtcc ggttgacctg gtgggcatcg
     9781 cgctgcagct cggtgccact tatgtggcgc gcagtttctc gggcgacaag gcccaactgg
     9841 tgccgctgat caagggtgcc atggcgcacg gcggtgccgc tttcattgat gtgatcagcc
     9901 cctgcgtcac cttcaacaac cacggcggca gcaccaagag ctacgactat gtgcgccagc
     9961 acaacgaagc ggtgagccgc atcgacttca tcacgccggg cagcgagatc acaaccgagt
    10021 atgggccggg cgaattggtc gaggtggaac aacacgatgg cagcagcctg cgcctgcgca
    10081 aactgcacac cgactacgac ccgaccgacc gctatgccgc catgagttac atgcattcgc
    10141 atgctgcgct gggcgaaatc gtcactggcc tgctctacat tgatccgctg gccactgatc
    10201 tgcacacctc aatgaacacg atagccggtc cgctcaacac cttgggcgcg gcacagctca
    10261 atccgggcca gaaagccctc gacaagatca acgctgcgct gcgctgaaat cttggcaacc
    10321 gcataccgtt caagttgccc ccaaggagcc ccgtcatgcc cgaacgtacc gtttttcaat
    10381 ccatggtgca acgtcacgtc attggcgtgc tgcccactgc caccgttcac actgctgcct
    10441 gcgtgatgac ccgcaacaac agcagcagcg tgctggtgat tgatgctgcc aacaccatgc
    10501 agggcatcat caccgaacgc gacatgatta ccagggtgct ggccaaggcg ctcgacccca
    10561 gccaaacccc ggtggccgag gtcatgacgc gcagtccgca ttgtgttttt ccggacacca
    10621 aagtggccga tgcggttctg atgatgattg agcggggctt tcggcatctg ccggtggtca
    10681 ccgccgacag ccacaagatt ctgggcgttt tctcggtacg tgatgcgat
//
LOCUS       DAOSLL010000075        10657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6096_length_10657_cov_6.434258, whole genome shotgun sequence.
ACCESSION   DAOSLL010000075 DAOSLL010000000
VERSION     DAOSLL010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10657
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6096_length_10657_cov_6.434258"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3449)
                     /locus_tag="PLB25_04900"
     CDS             complement(<1..3449)
                     /locus_tag="PLB25_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOE40953.1"
                     /translation="MTERYASLNASERALIAILALAGETPGKGRLVNYLRDAGIKSPQ
                     GSGYTPATLDAVLTDLKRLGWVVQTPSGDLSCLPSLQATALQDAAASAILNRLGEAVH
                     AVEGVNLQSGVAFPRTFRNAMRMLRMAMLLGWEPARVRKRLELCADHHDFYQQHPYLT
                     LFARPFDPALFALLHPLVQEEVLIELLPDCLAHLAPVEPLNHCAQALLAQPTEHYPPF
                     ISACAEHFLLCGQLDVAAAVSEPRQDITGQVLRSTVVLLKGDLGAAIGGFETALKTYR
                     QNSGSRNAIFTGLCGYLHVLALLRSDDDKLKKRAQAYLETAMRMPGANASAFACLTYL
                     NLVQTGQQKVSGSKQQVSPQAPLSQLIAHLVDFWLGAAQTEMKTLLLMELFLKSEAAG
                     FHFITMQAAALLERTSDKLRAPDMADHQARAAALRTQHGMVCMTDWFALLEPWERQLA
                     ALAKLSPSGKDAGSGTAQSRLIWELNFGPGYASLAPREQKRKGKAAWTGGRAVALKRL
                     SEEADTMDYLTPQDRRVCQSISKASYGYYGSTDYLLDGMGALPHLIGHPLVFLTDPAG
                     VRVEILRGEIELEIKKTAGNTLTLRLQPLIEKADGDVLVTKETPTRLRVMEVTDEHRR
                     IGAILGKSLKVPARAKEQVLDAIRAISSLITIQSDLEVLPSNMAQVEADPRLHVHLMP
                     HGDGLRITLLVRPFANAGPYYAPGAGAESVIAEIAGQPLQARRDIAHEASAAKALIAN
                     CPALRDAIEAHGERLIEEPETCLELLLQLQGQGDRVLIAWPEGEKFKVSRELGGGRFS
                     LSIKRDVDWFSATGELQVNDEQVIDLQKLLALTAAGRGRFIHLGDNQFLALTDEFRRR
                     LDDLRSFSEHQGKGVRIHALAAGALQDLADAAGKLKTDKHWKDHLARLSELDALTPVL
                     PATLQAELRDYQLSGYNWLCRLAHWGVGACLADDMGLGKTLQALALLLARAADGPALV
                     VAPTSVCMNWLGEIDRFAPTLNVILFGAGDRQLTLSELKSFDLVVVSYGLLQQEAERF
                     GAVHWHTIVLDEAQAIKNHQTKRSQAAMALQGNFRMVCSGTPLENHLGELWNLFRFIN
                     AGLLGSLEQFNERFAGPIERLQDAGARNRLRRLIGPFMLRRTKSQVLSELPSRTEILR
                     QVE"
     gene            3730..4371
                     /locus_tag="PLB25_04905"
     CDS             3730..4371
                     /locus_tag="PLB25_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOE40954.1"
                     /translation="MVMQSVAWLSAVLVFLSFFMKTIIPLRTIAIVSNFVFIAYALLG
                     IHYGVFDKVLPILVLHVALLPLNLLRLKQVRTAIKSLHDSNNREQTLEFLIPYMKTES
                     YPRGHRLFSQGDDACCMYLIRSGQLELPEIGKLLGPGVALGEVGIFAEHARRTTSAVC
                     TQDCELYSVTADKVMELFYQEPRFGLYIVRAMSRYLANEQLSATNAAPASPSP"
     gene            complement(4529..5545)
                     /locus_tag="PLB25_04910"
     CDS             complement(4529..5545)
                     /locus_tag="PLB25_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE40955.1"
                     /translation="MIRSSKFTSSLLAAALAVCGISASVSASAADKVTVQLKWLPQAQ
                     FAGYYVAQSKGYYKEAGLDVTIKPGGPDISPVQVIAGNGADVVVNWMPDALAAREAGV
                     PLVNIAQVFNQSGLMLTCKKASGVSSPKDFKGKTLGVWYGGNEYPFLNWMAKLGLKTD
                     TDIKILKQGFNVDPLLQNQAACISTMIYNEYWQVVDAGVKESDLVTFFYEKEGVASLE
                     DGLYVMEAKLKDPAFVARMGKFLKATFKGWNDAVKDPAGAAKIVVAADASGSATEKVQ
                     KRQMENVAKLITNAGTPRIGYLEPAAYERTVKVLLAAGSSPVIKKDPGKAAYSHVVWE
                     ASAK"
     gene            complement(5599..6558)
                     /locus_tag="PLB25_04915"
     CDS             complement(5599..6558)
                     /locus_tag="PLB25_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE40956.1"
                     /translation="MNRPLANTLLFVGVGLTSAAVLMMQPAALGQLPEGTTPGALFWL
                     ATLALAGLAIQSVRLMAAVSGGRWVGSATAALFGVWIVYFWQLLVVAFEVPRVLLPPP
                     SLIVQAFARHSEVLWGDFVQTVLKAVLIGWGLGSGLGFMVAVAIDRQPFLQRGLLPLA
                     SLTSTIPLVAVAPIAVMWFGFEWPSKAAVVVLMTFFPMLVSTLAGLKASGKLERELMY
                     SYAAGYSRTLLALRLPSALPFIFGALKINATLALIGAIVAEFFGSPTSGLGFRISTEA
                     SRMNMPLVWSAIVVAAVTGSVAYAILVQFERRAAFWHPSVRGA"
     gene            complement(6555..7448)
                     /locus_tag="PLB25_04920"
     CDS             complement(6555..7448)
                     /locus_tag="PLB25_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE40957.1"
                     /translation="MTAKASQWQRLQRDGLPVLVMLVAALLLWYAGAVWLNAAGAIER
                     VLPTDVPWTWQQLLAATMDMQRPVLPTPHQVGLDFWSSLVDWPLDSPRNLLFHVAVTA
                     ESTLWGFVMGTLLGLLLAVLIVHSRTLDRALLPWIVASQTVPVLAIAPIVLVILGSLG
                     FAGVAPKAVIAMYLCFFPVTVAMVQGLRSPQRIETEMMHTYAANWWQALWLLRLPASL
                     PFLFPALRVGIAAGLVGAMVAELPTGAVAGLGARLLTGSYYGNTVQIWSALVMSALLG
                     LALTTAVAGIERLVLRRRGQP"
     gene            complement(7524..8351)
                     /locus_tag="PLB25_04925"
     CDS             complement(7524..8351)
                     /locus_tag="PLB25_04925"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE40958.1"
                     /translation="MNHPEDSPIISRLNPPAGGRLAVEIRQTSVVYQTADSPVHALEN
                     IDLNIHEGEFVSLIGPSGCGKTTLVRVIADLEQISAGLVLVNGVSPHEARLARAYGYV
                     FQSPALFPWRTVLANVTLPLQIQGRTKADAKAIALEHLERVGLVGFDGKYPWQLSGGM
                     QQRVSIARALSFEPRILMMDEPFGALDEITRDRLNEQLQQLWQRERRTVVFVTHSIAE
                     AVYLSTKIVVMSPRPGRIVKVIESPLPDDRHLAMRDTSTFIQVAHEVREALADGHHD"
     gene            8551..9021
                     /locus_tag="PLB25_04930"
     CDS             8551..9021
                     /locus_tag="PLB25_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HOE40959.1"
                     /translation="MSLPHEYHCRLGGSLLVLLQFGLLLWLAVMAVPKFLQGPVGSAC
                     WLLAALSAALGAWTLLHNRLGNFNIHPAPKAGGLLVTSGPYRWMRHPMYSSVLLGAAA
                     MSCLSDGLTAGLVWAALLLVLLVKSGLEEHWLRLHHPQYQAYCQQCKRFVPGIL"
     gene            complement(9180..9836)
                     /locus_tag="PLB25_04935"
     CDS             complement(9180..9836)
                     /locus_tag="PLB25_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40960.1"
                     /translation="MKKLLLLVAAVSAASLAGAQEMGRVISSTPIIEQVGVPRQVCST
                     EQVAVQQGNSGAGAVMGAIAGGAVGNTVGRGSGRAAATMLGLVGGAIVGNRIEGGTTA
                     QLQNVQRCSTQTVFENQPVAYNVVYEYAGRQYRVQMPNDPGPTIALTVRPVGVSVPAP
                     ASLHSTGYQQQPGLQPANVVVDAPLRANDERRFYRQPYPAPIANDDNWDERTDDRYWR
                     "
     gene            complement(9969..10145)
                     /locus_tag="PLB25_04940"
     CDS             complement(9969..10145)
                     /locus_tag="PLB25_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40961.1"
                     /translation="MANMDQHRFVAKTSWLRPYTRWAARFALASPRWVPAALRNTPLK
                     LSSLILRGYHRALR"
     gene            complement(10145..>10657)
                     /locus_tag="PLB25_04945"
     CDS             complement(10145..>10657)
                     /locus_tag="PLB25_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HOE40962.1"
                     /translation="LARTAIDSTSSSSYACDLKKWLQIMEAYESGGHAYHATMPTDAL
                     VRLREVMQETRDYGFEKVRAEQFALGKKVRALFESRGIASVAANGFKAPGVVVSYTTD
                     PDIQNSKKFLALGLQTAAGVPLQCDEPADFMSFRVGLFGLEKLHNVDRSVAQLATALD
                     ALGIRAPETV"
BASE COUNT         2096 a         3301 c         3189 g         2071 t
ORIGIN      
        1 aactcgacct ggcgcaagat ttcagtgcgc gacggcagct cggacaacac ctgcgacttg
       61 gtacgccgca gcatgaacgg gccaatcagc cgacgcagcc ggttgcgcgc accggcatcc
      121 tgcagccgct cgattggccc ggcgaagcgt tcgttgaatt gttccagcga acccagcagg
      181 ccggcgttga tgaaacgaaa caggttccac agttcgccta gatggttttc cagcggcgtg
      241 cctgagcaaa ccatgcggaa attgccttgc agcgccatgg ctgcctgcga gcgcttggtc
      301 tggtggtttt tgatggcctg cgcctcgtca agcacgatgg tgtgccaatg caccgcgcca
      361 aagcgctcgg cttcttgttg cagcaggcca taactgacca ccaccaggtc aaacgacttg
      421 agttcggaca gtgtcaactg gcggtcaccg gcgccaaaca gaatcacgtt gagggtcggt
      481 gcaaagcggt ctatttcacc cagccagttc atgcagaccg aggtcggtgc caccaccagc
      541 gccgggccgt ctgccgcacg cgccagcagc agcgccagcg cctgcagggt tttgcccagg
      601 cccatgtcgt cggccagaca ggcaccgaca ccccagtgcg ccagtcggca cagccagttg
      661 tagccgctta actgatagtc gcgcagctca gcttgcaagg tggctggcag caccggtgtc
      721 agggcgtcga gttcgctcag gcgcgccagg tggtctttcc agtgcttgtc ggtcttgagt
      781 ttgcccgccg cgtcggccag gtcttgcagc gcaccggcgg ccagtgcgtg gatgcgcacg
      841 cctttgccct gatgttcgga aaatgatcgc aggtcatcga gtcggcgacg aaattcgtcg
      901 gtcagggcca gaaactggtt gtcacccaga tggatgaaac ggccgcgccc ggcagccgtc
      961 agcgccagca gcttctgcag gtcaatcacc tgttcgtcgt tcacctgcag ctcaccggtg
     1021 gcagaaaacc agtcgacatc gcgcttgatc gacagagaaa agcggccacc accgagttcg
     1081 cggctgacct tgaatttctc gccctcgggc caggcaatca gcacccggtc accctgcccc
     1141 tgcagttgca gcagcagttc caggcaggtt tcgggttctt caatcaggcg ttcgccatgc
     1201 gcttcaatgg catcgcgcag ggccgggcag ttggcgatca gcgccttggc ggcgctggcc
     1261 tcatgggcaa tgtcgcggcg tgcctgcagc ggctggccgg cgatttcggc aatcacgctc
     1321 tcggcacccg caccaggtgc gtagtacggg ccggcgttgg caaaggggcg caccagcaag
     1381 gtgatgcgca gcccgtcgcc atgcggcatc aggtgcacat gcaagcgggg atcggcctca
     1441 acctgcgcca tgttggacgg cagcacctcc aggtctgact ggatggtgat cagcgacgag
     1501 atcgcgcgaa tggcgtcgag cacctgctct ttggcgcgcg ctggcacctt gagcgacttg
     1561 cccagaatgg cgccaatgcg ccggtgctcg tcggtcacct ccataacccg cagccgggtc
     1621 ggggtttcct tggtgaccag cacgtcgccg tcggccttct cgatcaacgg ctgcaggcgc
     1681 agcgtcaggg tgttgcctgc ggttttcttg atttcgagtt cgatctcacc acgcaaaatc
     1741 tcgaccctga cccctgccgg atcggtcaga aacaccagcg gatggccaat caggtgtggc
     1801 agtgcgccca tgccatcgag caaatagtcg gtgctgccgt agtagccgta gctggctttg
     1861 ctgatgctct ggcacacccg gcggtcttgc ggcgtcaggt aatccatcgt gtcagcctct
     1921 tcgctcaggc gcttgagtgc taccgcgcgg ccaccggtcc aggcggcctt gcctttgcgt
     1981 ttttgctcac gcggtgccag cgaggcgtag ccaggcccaa agttcagctc ccagatcagg
     2041 cgggactgcg ctgtcccgct gcctgcatct ttgccggacg ggctcaattt ggctaatgcc
     2101 gccagttggc gctcccacgg ctccagcagg gcaaaccagt cggtcatgca gaccatgcca
     2161 tgctgtgtgc gcagggcggc ggcgcgggcc tggtggtcgg ccatgtctgg tgcgcgcagc
     2221 ttgtcgctgg tgcgctcaag caacgcggcc gcttgcatgg tgatgaaatg aaaacctgcg
     2281 gcctcacttt tgaggaacag ctccatcagc aacagggttt tcatctcggt ctgcgctgca
     2341 cccagccaaa aatcaaccag gtgtgcaatc aactgcgaca gcggggcctg cggcgaaacc
     2401 tgttgcttgc tgccgctgac cttctgttgg ccggtctgga ccaggttcag gtaggtcagg
     2461 caggcgaagg ccgacgcatt ggcaccaggc atgcgcattg ccgtttccag ataggcctgc
     2521 gccctttttt tcagcttgtc gtcgtcgctg cgcaacaagg ccagcacatg caaatagcca
     2581 cataggccgg taaaaatggc attgcgcgag ccactgtttt gtcggtatgt tttgagcgcg
     2641 gtttcaaacc cgccaatggc agcgcccagg tcgcccttga gcagcaccac ggtgctgcgt
     2701 aacacttgcc cggtgatgtc ctggcgcggt tcgctcaccg ccgccgccac gtcgagctga
     2761 ccgcacagca gaaagtgctc ggcgcaggcc gaaatgaacg gcgggtagtg ctctgtcggc
     2821 tgcgccagca aagcctgcgc gcaatggttc agcggctcga ccggtgccag atgcgccagg
     2881 cagtcgggca gcagttcaat cagcacttct tcctgaacca gcgggtgcag cagcgcaaac
     2941 aaggctgggt caaacggtcg cgcaaacagt gtcaggtaag gatgctgttg gtaaaagtcg
     3001 tggtggtcgg cacatagctc aaggcgtttg cgcacccgcg caggctccca acccagcagc
     3061 atggccatgc gcagcatgcg catggcgttg cggaaagtgc gcggaaaagc cacaccactc
     3121 tgaagattga ccccttcgac cgcatgcacc gcttcgccaa ggcggttcaa gatggcgctg
     3181 gctgctgcgt cttgcaaggc ggttgcctgc aacgacggca gacagctcag gtcaccactg
     3241 ggtgtctgca ccacccagcc gaggcgcttc aggtcggtca gcaccgcatc cagcgtggcg
     3301 ggggtatagc cgctgccctg tggcgacttg atgccggcat cgcgcaggta gttgaccaga
     3361 cgacccttgc cgggcgtttc tccggccagg gccaggatgg cgatcagggc tcgttcggag
     3421 gcgttcaggc tggcgtagcg ttctgtcatg gggctggctt tatcaggtag ggcgggtgct
     3481 gcggttgaag ggtagatcag ccaaccaagc aaaccgcgca ggtgtcgggt ttatgataaa
     3541 aatacggctg tagcccttgc gcagattgcg taacgtgcta tctattttga attaggtgat
     3601 tgatcacaaa gctgcgcagg ataaaagtcg ccatggtatc tcaacagtat ccggtacatt
     3661 tccttcctgg ttcaaaacaa tcgatagatc ggcaacagca cgcttttgcc acatagcagg
     3721 agacccccca tggtgatgca gtctgtcgcc tggctatcag cagtcttggt gttcttgtcg
     3781 tttttcatga agaccatcat tccgctgcga accattgcca tcgtcagcaa ttttgtcttt
     3841 atcgcctatg ccctgctggg cattcactac ggtgtatttg acaaggtgtt gccgattctg
     3901 gtgctgcatg tggcgctgct gccgctgaac ctgctgcgcc tcaaacaggt cagaaccgcc
     3961 atcaaaagcc ttcatgacag caacaatcgg gagcaaaccc ttgagttttt gattccctac
     4021 atgaaaaccg agtcgtaccc caggggacac cggttgttca gccagggcga cgatgcttgc
     4081 tgcatgtacc tgattcgcag cggtcagctt gaactgccgg aaattggcaa attgcttggc
     4141 cccggtgttg ccttgggcga agtgggcata tttgccgagc acgcccggcg aaccacttct
     4201 gccgtctgca cccaggactg cgaactctac agcgtgaccg ccgacaaggt gatggagctg
     4261 ttttaccagg agccacgttt tgggctgtat atcgttcggg ccatgtcacg ctatctggca
     4321 aacgagcaac tcagcgctac caacgcggca ccggcaagcc catcgccttg agccggttca
     4381 cggcaaggct gactggccca gcgctggaaa agaccgtttt ggtggccgcc agcatcaagg
     4441 cctgattgac ctggcaaaac tggcctttgc gggtgtcgtt ggctggcgcg gctggtggtc
     4501 agggccgcgc cgcgcacgtg ctgttggcct atttggcaga ggcttcccac accacgtgtg
     4561 agtaggcagc tttgcccggg tcttttttga ttaccggcga gcttcctgcg gccagcagca
     4621 ccttgacggt acgctcatag gcggcaggct ccaggtaacc gatcctgggt gtaccggcat
     4681 tggtgatcag cttggcaacg ttttccatct ggcgtttctg aactttttct gtggcactgc
     4741 cagaggcatc agccgccacc acaatcttgg cggcaccagc ggggtctttc acggcatcat
     4801 tccagccttt gaaggtggcc ttgagaaact tgcccatgcg cgccacaaag gccgggtcct
     4861 tcagcttggc ttccatcaca tacaagccgt cttccagcga tgccacgcct tctttttcat
     4921 agaagaaggt gaccaggtca ctttccttga caccggcatc caccacttgc caatattcgt
     4981 tgtagatcat ggtcgagatg cacgctgcct ggttttgcag cagcggatca acgttaaagc
     5041 cttgcttgag aatcttgatg tcggtgtcgg ttttcaggcc cagtttggcc atccagttca
     5101 ggaacgggta ctcgttgccg ccgtaccaga cacccagggt cttgccttta aagtctttcg
     5161 ggctgctcac cccgctggcc tttttgcagg tcagcatcag gccggactga ttgaacacct
     5221 gggcaatgtt gaccagcggc acaccggctt cgcgcgcggc cagcgcatca ggcatccagt
     5281 tcaccaccac atcagcgccg ttgccggcaa tcacctgcac cggcgaaatg tccggtccac
     5341 cgggtttgat ggtgacatca agcccggctt ctttgtaata gcccttgctt tgcgccacgt
     5401 agtaaccggc aaattgcgct tgcggcaacc acttgagctg cacggtgacc ttgtcggctg
     5461 cgctggcact gacactggcg gagataccgc agacagcgag tgccgcagcc agcaggctgc
     5521 tggtgaactt ggaagaacga atcatgggag tagctccttt ggggggtgga aaaaacagga
     5581 cacgcatagg gcctagtatc aggcaccgcg cactgacggg tgccaaaacg cggcgcggcg
     5641 ctcaaactgg accaagattg cataggccac cgacccggtg accgcagcca ccacgatggc
     5701 gctccagacc agcggcatgt tcatgcgcga ggcctcagtc gaaatgcgaa agcccaggcc
     5761 tgacgtcggt gagccaaaga actcagcgac gatggcaccg atcaaggcca gcgtggcatt
     5821 gatcttgagc gcgccaaaga tgaacggcaa ggccgacggc aggcgcagcg ccagcagggt
     5881 gcggctgtaa ccggcggcgt agctgtacat cagctcgcgt tcgagcttgc ccgatgcctt
     5941 gagcccggcc agcgttgaca ccagcatcgg aaaaaacgtc atcagcacca ccacagccgc
     6001 cttggacggc cactcaaaac caaaccacat caccgcaatc ggtgccaccg ccaccagcgg
     6061 gatggtgctg gtcagcgatg ccagtggcag cagcccgcgt tgcaaaaacg gctggcggtc
     6121 aatcgccacg gccaccataa agcccagccc cgagcccaaa ccccagccga tcagcacggc
     6181 tttgagcacg gtttgcacaa agtcgcccca caacacctcg ctgtgcctgg caaaggcttg
     6241 cacgatcagg ctgggtggcg gcagcagcac ccgtggcacc tcaaaggcca ccaccagcag
     6301 ttgccaaaaa tagacgatcc acacgccaaa cagggcggcg gtggcgctgc caacccaacg
     6361 gcccccgctg accgccgcca tcaagcgcac cgactgaatt gccagcccgg ccagcgccag
     6421 ggtggccagc cagaacagtg cgccaggtgt cgtgccttca ggcagttgtc ccaaagctgc
     6481 aggctgcatc atcagcaccg ctgcgctggt cagaccgaca ccgacaaaca gcagggtgtt
     6541 ggcgagtggg cgattcatgg ctgcccccgg cggcgcagca ccagccgctc aatgcccgcc
     6601 accgcagtgg tcagtgccag cccgagcagc gccgacatca ccagcgccga ccagatttgc
     6661 acggtgttgc cgtaatatga gccagtcagc aggcgggcgc ccagaccggc caccgccccg
     6721 gttggcagct cggccaccat ggcacccacc aggcccgccg caatgccaac gcgcaaagcc
     6781 ggaaacaaaa atggcagcga ggctggcagg cgcagcagcc acagcgcctg ccaccagttg
     6841 gcggcatagg tgtgcatcat ttcggtttcg atgcgttgcg gtgagcgcaa gccctgcacc
     6901 atcgccacgg tgaccggaaa aaagcacaaa tacatcgcaa tcaccgcttt cggggccacc
     6961 ccggcaaagc ccagactgcc cagtattacc agcacaatcg gcgctattgc cagcaccggc
     7021 acggtttgcg aggccacaat ccagggcagc aaggcccggt cgagcgtgcg cgagtgcacg
     7081 atcagcaccg ccagcaacag gcccagcagg gtgcccatga caaatcccca cagggtggac
     7141 tcggctgtga cggccacatg aaacagcagg ttgcgcggcg aatctagcgg ccagtccacc
     7201 agactactcc agaaatccag gccgacttga tgtggcgttg gcaacaccgg gcgctgcatg
     7261 tccatggtgg ccgccagcag ttgttgccag gtccagggta catcggtcgg caacacccgc
     7321 tcaatggccc cggcagcatt gagccacact gcgcccgcat accaaagcag caaagccgcc
     7381 accagcatga caagcaccgg caggccatcg cgctgcaggc gctgccattg acttgctttg
     7441 gcggtcatgg ctgcaaggcc cgccccagcg caaggcaggc gggcgagtcg gctgagcttg
     7501 ccgggcgttg ggtcatgcca ggctcagtca tgatgaccat ccgccagcgc ttcgcgaacc
     7561 tcgtgggcca cctgaatgaa ggtgctggtg tcgcgcatgg ccagatgccg gtcatcgggc
     7621 agcggcgact caatcacctt cacaatgcgc cctgggcgcg gcgacatcac gacaattttg
     7681 gtggacaggt acaccgcctc ggcaatcgag tgggtgacaa acaccacggt acggcgctcg
     7741 cgctgccaca gttgctgcaa ttgttcattc aggcggtccc gggtgatctc gtcaagcgcg
     7801 ccaaacggct cgtccatcat caaaattctg ggctcaaacg acaaggcgcg cgcaattgac
     7861 acacgctgct gcatgccgcc cgaaagttgc caggggtatt tgccatcaaa accgaccagg
     7921 ccgacgcgct ccagatgctc cagtgcaatg gccttggcat cggctttggt gcggccctga
     7981 atttgcaagg gcagtgtcac attggccagc acggtgcgcc acggaaaaag tgccggggac
     8041 tgaaacacat agccgtaggc gcgtgccagg cgggcttcat gtggcgacac cccattgacc
     8101 agcaccaggc ctgcgctgat ttgttccagg tcggcaatca cccgcaccaa ggtggtcttg
     8161 ccacagccag acgggccaat caacgagaca aattcacctt cgtgaatgtt cagatcgatg
     8221 ttctccagcg catgcacggg tgaatctgcc gtctgataga ccacgctggt ctggcggatt
     8281 tcaaccgcca gacgaccgcc tgcgggcggg ttcagcctgg aaatgatggg cgagtcttcg
     8341 gggtggttca tgggtacaca aaaagggttg actgcaccaa aaatgggcat tgtcgccaag
     8401 aagttcacca gtcggtcaac taattgcacg atcccaagcg attttcatgc caaacgggtg
     8461 tttgtcatca tgcggtcggc cctggcgatc agcctgactg ccccatcggc gacaatagtt
     8521 tgtctgagta gtcaaccccg tatgaaacca atgtcacttc cccacgaata ccattgtcgg
     8581 ctgggcgggt cgttgctggt gctgttgcag tttggcttgc tgctgtggct tgccgtcatg
     8641 gctgtgccga agttcttgca aggcccggtt ggcagcgcct gctggctgct ggccgccctg
     8701 tccgcagctc tgggcgcctg gaccctgctg cataaccggc taggcaattt caacattcac
     8761 ccggcaccca aagccggcgg gttgctggtc accagcggac cctaccggtg gatgcgccac
     8821 cctatgtaca gctcggtttt gctcggggca gcggccatgt cgtgcctgtc agatggactg
     8881 accgccgggc tggtctgggc ggcgctgttg ctggtgctgc tggtcaagtc cggcctggaa
     8941 gagcactggc tgcgcctgca ccacccgcag taccaggcct attgccagca gtgcaagcga
     9001 ttcgttccag ggattcttta ggatgtggtc gggaataact ccccgttttg caccaaggag
     9061 ttgggatgca cggctaggcg caagacgcgc cgtttagctg gctaaacaag cgtcttgcaa
     9121 cgacgctgtg cgcccaaatc cgccggtgca aatgggggaa gttgtttctg accacatcct
     9181 tagcgccaat accgatcgtc cgtgcgctca tcccagttat cgtcattggc aattggggcc
     9241 gggtagggtt gacggtaaaa gcgtctttcg tcattcgccc gcagcggtgc atcaaccacg
     9301 acatttgctg gctgcaggcc gggctgttgc tgataaccgg tgctgtgtag cgatgccggg
     9361 gctggcacgc tgacaccgac cgggcgcacc gtcagcgcaa tcgttggccc cgggtcattg
     9421 ggcatttgca cccggtattg gcgcccggca tattcataaa ccacgttgta cgcgacaggc
     9481 tggttctcga aaacggtttg agtgctacat cgctgtacgt tttgcaattg agcggtggtg
     9541 ccgccttcga tgcggttacc cacaattgcg ccgcccacca ggccaagcat ggtggcggcc
     9601 gcccgcccac tgccacggcc taccgtattg cctactgcac caccggcaat ggcccccatt
     9661 accgcacccg cccccgagtt gccttgctgc actgcgacct gttcggtgct gcacacctga
     9721 cgcggcacac cgacttgttc aatgatcggc gttgaggaaa tcacccggcc catttcctga
     9781 gccccggcga gcgaagccgc tgacactgcg gcgaccaaca agagcaactt tttcatgcaa
     9841 ttcacctttt gtttgttaaa ccgggcgtat cgtgcccgcc cacaccgggt gcctgggttg
     9901 cagttgcgta aagctcgggt aaagagtgtc accgggtgtt ttttcaaccg ggcgacgggt
     9961 tgggtggctt agcgcaaggc tcggtggtag ccgcgcaaaa tcaacgacga cagcttgagc
    10021 ggggtgttgc gcaacgcggc tggcacccat cgaggtgaag ccagcgcaaa gcgcgctgcc
    10081 cagcgcgtgt aggggcgcag ccaactggtt ttggcaacaa aacggtgctg atccatgttg
    10141 gccatcaaac cgtttcaggc gcccggatgc ccagcgcatc cagcgctgtg gccagttgcg
    10201 ccacactgcg gtccacgtta tgcagttttt ccagcccgaa caggccaacc cgaaaactca
    10261 tgaaatctgc tggctcatcg cactgcagtg gcacgccagc agccgtttgc aggccaagtg
    10321 ccaggaattt cttgctgttc tgaatgtcgg ggtcggtggt gtagctaacc accacaccgg
    10381 gtgccttgaa gccgttggcc gccacactgg caataccccg gctctcgaac agggcgcgca
    10441 ctttcttgcc cagcgcgaat tgttcggcgc gtactttttc aaagccgtag tcgcgggttt
    10501 cctgcatcac ctcgcgcagc cgcaccagcg catcggtggg catggtggca tggtaagcat
    10561 ggccacccga ctcataggct tccatgattt gcagccactt tttcaggtcg caggcatagc
    10621 tgctgctgct ggtgctgtca atggccgtgc gggccag
//
LOCUS       DAOSLL010000076        10605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6130_length_10605_cov_4.138957, whole genome shotgun sequence.
ACCESSION   DAOSLL010000076 DAOSLL010000000
VERSION     DAOSLL010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10605
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6130_length_10605_cov_4.138957"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="PLB25_04950"
     CDS             <1..692
                     /locus_tag="PLB25_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HOE40963.1"
                     /translation="ADVVVAGGAEATVSALGVGGFAAMRALSIRNDDPTTASRPWDKD
                     RDGFVLGEGAGVMVLEEYEHAKARGAKIYAELAGFGMSADAGHMTAPNLDGPRRAMLI
                     AMDNAGVNANQIDYVNAHGTSTPLGDLNESNAIKAALGEQAKKIVVNSTKSMTGHLLG
                     GAGGLESVVTVLALHHQKSPPTTNIFNIDPECDLDYCANTARDMKIDVALKNNFGFGG
                     TNGALVFKRAP"
     gene            739..1218
                     /locus_tag="PLB25_04955"
     CDS             739..1218
                     /locus_tag="PLB25_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40964.1"
                     /translation="MHSAPAVTYPVGRSHFHAALVALITLAGLATLGCWLQQADAIGA
                     RHLVAVALWLTSSGLAAWQVWHAPAGVLTWDGLGWTWTCAELSQSVVLEVTLDLQAYL
                     LLRLHVSDGKRRLWAWPEMRTSPLRWLTFRRAVFGKMAAQADLTDPAGLVASARMPP"
     gene            1275..1859
                     /gene="rpoE"
                     /locus_tag="PLB25_04960"
     CDS             1275..1859
                     /gene="rpoE"
                     /locus_tag="PLB25_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HOE40965.1"
                     /translation="MLVERTVAGDQAAFGLLVIKYQRRIERLIGRMVRDVDLVEDIAQ
                     ETFIRAYRALPKFRGDSQFYTWLYRIAINTAKKFLLELKRDPTVSQSTLERRSEEDET
                     FQPKSEPSTEETPESVLAAKEIGQAVNAVLDALPVDLRQAITLREMDGLSYEEIAEVM
                     NCPVGTVRSRIFRAREAVSIRIKPMLEKQTSKRW"
     gene            1919..2524
                     /locus_tag="PLB25_04965"
     CDS             1919..2524
                     /locus_tag="PLB25_04965"
                     /inference="COORDINATES: protein motif:HMM:NF015810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HOE40966.1"
                     /translation="MSDNLHRDSELVSLLVDGRLRGAQFGRAVEHLSASADARQAWDS
                     YHLIGEVMRSGPAQARGHDPAFVQRLLNRISTEATKNIAIDALSIRAEGQNTSKPRAA
                     NDGWWLRIAGIASVALVGVLAWQGYLLMDQPAVTLVSSPATANPATAPVAMASAEASG
                     SMLRDPRLDALMAAHRQFGGASALQMPSGFLRNATFDEGER"
     gene            2521..3537
                     /locus_tag="PLB25_04970"
     CDS             2521..3537
                     /locus_tag="PLB25_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HOE40967.1"
                     /translation="MKRLLVVLLLALCGSSLIGAPQAVAGSGPAHIAAEPSVDQWLMR
                     LHAASRGRAYMGTFVVSTDRSMSSSRIWHVCNGQQQVERVEALTGEPRSTYRRNDQVV
                     TFLPGRRVVVHEKRESLGLFPDLLGRADSSLARYYRLKTLGRDRVAGLDADMAQLIPF
                     DALRFGYRIWTEHQTGLVVKLQTLDKTDAVLEQAAFSELQLNAPVAMSRLSAQMDNRQ
                     GYRLRVAELVKTTAAQEGWLLKALVPGFRAISCNKQAETNMARDGGRALQCVFSDGLA
                     SVSLFMEPFDASQHSTAQQRAPVGMGATQVLTQRVGDWWLTAVGEVPYQTLILFAQGL
                     ERTK"
     gene            3579..5039
                     /locus_tag="PLB25_04975"
     CDS             3579..5039
                     /locus_tag="PLB25_04975"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HOE40968.1"
                     /translation="MIRLNLNRLFALALSGVLLTTAATALLPMSPAQAQARALPDFTD
                     LVEQVGPSVVNIRTLEKVSAEAGSGSPGEKEMQEFFRRFGLPMPNLPRSGPRRGQPQD
                     EEQPRGVGSGFVLTADGMILTNAHVLEGADEVMVTLTDKREFKARIIGADKRSDVAVV
                     KIDATGLPAVKVGDVGRLKVGEWVMAIGSPFGLENTVTAGIVSAKQRDTGDYLPFIQT
                     DVAINPGNSGGPLINMRGEVVGINSQIYSRSGGSMGISFAIPMDEALRVSEQLRASGR
                     VTRGRIGVQIGPVSKDVAESIGLGKPQGALVNGVEAGAPADKAGVEAGDIIIKFDGKA
                     VEKSSDLPRMVGSTKPGTKSTLTVFRRGSTKELSVVIAEIEAEKSAKKTTEKDDKPKH
                     PHIGQSIGLAVSELTKEQKGALSGHGGVRVDSAVDNAARAGIREGDIIMQLGNLVVSD
                     IKSFEAALAKVDKTKPIGVLVRRGELAQIVVIRPNK"
     gene            5627..7438
                     /gene="lepA"
                     /locus_tag="PLB25_04980"
     CDS             5627..7438
                     /gene="lepA"
                     /locus_tag="PLB25_04980"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HOE40969.1"
                     /translation="MNHIRNFSIIAHIDHGKSTLADRLIQRCGGLQEREMQAQVLDSM
                     DIEKERGITIKAQTAALRYQAKDGQIYNLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTALDLGVEVVPVLNKMDLPNADPDNARSEIEDVIGIDAVNAIA
                     CSAKTGMGIEDILEAVVARIPAPKGNPAGPLRAMIIDSWFDSYVGVVMLVRVVDGRLA
                     KGERIKMMATETTYNADGLGVFTPANEPREALNAGEVGYVIAGIRELQAAKVGDTITL
                     IKPGTGGLAFTATEPLPGFKEIQPQVFAGLYPTEANQYEGLRDSLEKLKLNDASLHYE
                     PEVSQALGFGFRCGFLGLLHMEIVQERLEREFDQDLITTAPSVVYQVVQADGAIKMVE
                     NPSKMPDQGRLDEIREPIVTVHLYMPQEYVGAVMTLSNQKRGVQMNMAYKGRQVVLTY
                     EMPLAEIVMDFFDKLKSVSRGYASMDYEFKEYRSADVVKVDILLNGEKVDALSIIVHR
                     AQSQYRGRQVVAKMREVISRQMYDVAIQAAIGSNVIARETIKALRKNVIAKCYGGDIS
                     RKRKLLEKQKEGKKRMKQIGSVEVPQEAFLAILKVDA"
     gene            7438..8403
                     /gene="lepB"
                     /locus_tag="PLB25_04985"
     CDS             7438..8403
                     /gene="lepB"
                     /locus_tag="PLB25_04985"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HOE40970.1"
                     /translation="MQAVTSVVLGAFVAYIGAWYFGQIEGNFALLLFLATVVTGVYWL
                     AERFYFLPQRQQAAATLETQALKRNEELGRMGIAKVDGDVAQAKRDILAQPWWLDWTA
                     GLFPVIIAVFVLRSFLFEPFKIPSGSMMPTLLVGDLILVNKYHYGLRLPVLNTRITEG
                     TAPQRGDVMVFRYPPKPSLDYIKRVVGVPGDEVAYLNKRLTINGKVQDTQALPEFFDE
                     DTMRYFKQFDEALGDKKHRVLNDENRPAFVPGADQFEFKTNCRYSVEGVVCKVPAGHY
                     FMMGDNRDNSLDSRYWGFVPDKNIVGKAFFVWMNFGNVKRIGAFN"
     gene            8417..8770
                     /locus_tag="PLB25_04990"
     CDS             8417..8770
                     /locus_tag="PLB25_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HOE40971.1"
                     /translation="MATHFRSSQRGLSFIGLVMIAVVVASTGLVLAQMVPTYIEFLAV
                     GKAAKKAASGTTIVDIRNTFDKAASIDNISAISGKDLDISKQGDKVVVAFAYQREIHL
                     AGPVYLLMKYEGKSQ"
     gene            8792..9478
                     /gene="rnc"
                     /locus_tag="PLB25_04995"
     CDS             8792..9478
                     /gene="rnc"
                     /locus_tag="PLB25_04995"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HOE40972.1"
                     /translation="MNADLHHLQKRLQHEFVDFNLLTLAVTHRSFSIDHNERLEFLGD
                     SVLGLCVADLLYERLSALPEGDLSRVRANLVRQETLHQLAVTLGLPDVIRLGEGEMRS
                     GGQKRPSILADALEALIGAVYLDGGFAAAKSLVRLLYQDVEVNPLMAAIGKDPKTELQ
                     EWLQGRRLKLPAYKVVATLGSAHQQTFDVECEIAELKLSERGIGGSRRSGEQAAATAM
                     LQTLKSLGKP"
     gene            9571..10503
                     /gene="era"
                     /locus_tag="PLB25_05000"
     CDS             9571..10503
                     /gene="era"
                     /locus_tag="PLB25_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HOE40973.1"
                     /translation="MRPAGPAQSPLEGQRCGLIAIVGKPNVGKSTLLNALVGQKISIT
                     SRKAQTTRHRITGIHTTGATQFVFVDTPGFQTLHGNALNKSLNKTVVGAVADVDLILF
                     IVEAGSFTPADARVLALLGKQIPTLLIANKLDHVHRRGDIAPWLQTMATKHAFAEFVP
                     MSAKNSKDVERLLGICEKYLPEQAWWYAADELTDRSERFMAAEMVREKLFRLTGDELP
                     YTSTVVIDKFEEEAGRGKQKRLLKIAATIVVERDSHKAMVIGDKGERVKRIGMETRVE
                     LEKLMDAKVFIEMWVKVRSGWADDEARVRSFGYE"
BASE COUNT         2162 a         2840 c         3323 g         2280 t
ORIGIN      
        1 atgccgatgt ggtggttgcc ggtggtgccg aggcgactgt gtcggctttg ggtgtcggcg
       61 gttttgccgc catgcgtgcc ctgagcattc gcaatgacga ccccacgacc gcctcgcgcc
      121 cctgggacaa ggaccgtgac ggtttcgtgc tgggggaagg tgccggtgtc atggtgctcg
      181 aagaatacga acatgccaaa gcccgcggtg ccaaaattta cgccgaactg gccgggtttg
      241 gcatgagtgc cgatgccggg cacatgaccg cgcccaatct ggacggcccg cgccgcgcca
      301 tgctgatcgc catggacaat gccggtgtca atgccaacca gattgactat gtcaacgcgc
      361 acggcacctc gacaccactg ggcgacctca acgaatccaa cgccatcaag gcggctttgg
      421 gcgagcaggc aaaaaagatc gtggtcaact ccaccaaatc aatgaccggg catctgctcg
      481 gtggtgccgg tggtctggag tcggtggtga cggtgctggc actgcaccac caaaagagcc
      541 cacccaccac caatatcttc aacattgacc ccgaatgcga ccttgactac tgtgccaaca
      601 cggcgcggga catgaagatt gacgtggcct tgaaaaacaa ttttggtttt ggtggaacca
      661 acggcgcatt ggtgttcaaa cgggcaccct gaagcttgct gatcgcgggc gacatttctt
      721 tcgatcatcg ctttcaccat gcacagcgcc ccagcggtga catacccggt ggggcgttcg
      781 cattttcacg ctgcgttggt ggcgctgata acactggctg gtctggctac cttgggctgc
      841 tggctgcagc aggcagatgc catcggggca aggcatcttg ttgctgtggc cctgtggttg
      901 acgagcagcg ggctggccgc ctggcaggta tggcatgccc cggccggagt cctgacttgg
      961 gacgggctcg gctggacctg gacttgcgca gagcttagcc agtccgttgt actggaagtg
     1021 acccttgatc tgcaggctta tctgctgctg cgtttgcatg tcagtgatgg caagcgccgc
     1081 ttatgggcct ggcctgaaat gcgcacctcg ccgctacgct ggctgacttt tcgacgtgcc
     1141 gtgtttggca agatggcggc gcaagccgac ctgactgatc cggcgggctt ggtcgcgtcc
     1201 gcccggatgc cgccatgagt ttgcctgata cctcggcatc cgggcatcag acgagcctgc
     1261 ctgacagcga cctgatgctg gtagagcgca ccgtggccgg tgaccaggcg gcctttggtt
     1321 tgctggtgat caagtaccag cgccggattg agcgactgat tggccgcatg gtgcgcgatg
     1381 tcgatctggt cgaagacatc gcccaggaga ccttcatccg ggcctaccgt gcgctgccca
     1441 agtttcgcgg cgattcccag ttttacactt ggctctatcg cattgcaatc aataccgcca
     1501 agaagtttct gcttgaactc aagcgtgatc cgacggtgtc gcaaagcacg ctggagcgcc
     1561 gttctgagga agatgaaact tttcagccca aaagcgaacc aagcacagag gaaacgccgg
     1621 aatcggtgtt ggcagccaaa gaaattggtc aggcggtcaa cgccgtgctg gacgcattgc
     1681 cggttgacct tcgtcaagcc atcacgttgc gggagatgga cgggctgagc tacgaagaga
     1741 ttgcagaggt gatgaattgt ccggttggca ctgtgcgttc gcggattttc cgggcgcgcg
     1801 aagcggtatc gatccggatc aagccgatgc ttgaaaagca gaccagcaag cgttggtagc
     1861 tttgaaaggt cggcaagacg tgtgtgatgt ggttcaagat tcttaaggca ctgtggaaat
     1921 gtccgacaac ttacatcgtg attccgaatt ggtatccctg ctggtcgatg gccgattgcg
     1981 cggtgcgcaa tttggccgcg cggtagagca cctgtcggca tcggcagacg cgcggcaggc
     2041 atgggacagc taccatctga ttggtgaagt gatgcgctcc ggaccagcgc aggcacgtgg
     2101 gcacgatcca gcttttgtgc agcgtctgct caatcgaatc agcacagaag ctacaaaaaa
     2161 tatagctatt gacgcactgt ccataagggc tgaaggccaa aatacatcca agccaagggc
     2221 agccaacgat ggctggtggt tgcgcattgc aggtattgcg tcggttgctc tagtgggtgt
     2281 gttggcgtgg cagggttacc tgctgatgga ccagcctgcg gtgacgcttg tgtcatcgcc
     2341 agctacagcg aatcctgcaa cagctcccgt tgccatggcc tcggctgagg cttctggcag
     2401 catgcttcgt gacccccggc ttgatgccct gatggcagcg catcggcagt ttggcggcgc
     2461 atcggctttg cagatgccgt cgggtttttt gcgcaacgcc acgttcgatg agggcgagcg
     2521 ttgaagcgtc tgctggttgt tttgttgctg gcgctgtgtg gcagctcgtt gatcggggct
     2581 ccgcaggcgg ttgccgggtc tggcccggca cacattgctg ccgagccgag tgtggatcaa
     2641 tggctgatgc gcttgcatgc agcttcgcgt ggacgcgctt acatgggcac ctttgtggtc
     2701 agcaccgacc gttcgatgtc gtcgtcacgc atctggcatg tttgtaacgg ccagcagcag
     2761 gtggagcggg ttgaggcgct gactggtgaa cctcgttcga cctatcggcg caatgaccag
     2821 gtggttactt ttttgccggg ccgccgggtg gttgttcacg aaaagcgtga gtcgctgggg
     2881 ctttttcccg acctgctggg tcgggctgat tcgtcgctgg ctcggtacta tcggctcaaa
     2941 acccttggac gtgacagggt agcagggctg gatgcggaca tggcgcaatt gataccattc
     3001 gatgccttgc gttttggcta ccggatctgg accgagcatc aaaccggtct ggtcgtcaag
     3061 ttgcagacgc tcgataaaac cgacgcggtg ctggagcagg cagcattttc cgaattgcag
     3121 ttgaatgcgc cagtcgccat gtccaggctg agtgcccaga tggacaaccg gcagggctac
     3181 cggttgcgcg ttgcagaatt agtaaagacc accgccgccc aggaaggctg gctgctgaaa
     3241 gcactggtgc cgggttttcg tgccatcagt tgcaacaagc aggccgaaac caatatggcc
     3301 agagacggtg gtcgcgccct gcaatgtgtt ttttcggacg gattggcttc ggtgtctttg
     3361 tttatggagc catttgatgc atcgcagcac agcaccgcgc agcagcgtgc gcctgttggc
     3421 atgggtgcga cccaggtgct gacgcaaaga gttggcgact ggtggctcac cgccgtgggc
     3481 gaagtgccat atcagaccct gatattgttt gcccagggtc ttgagagaac caagtaagtc
     3541 actatgtacg cgcttgtttt tatctggaaa ggctgaagat gattcgattg aatttgaacc
     3601 gtttgtttgc gcttgcgttg tccggggtgc tgctgaccac cgccgctacg gccttgttgc
     3661 cgatgagccc ggcgcaggcg caggcgcgcg ccctgccgga tttcactgac ttggtggagc
     3721 aggttgggcc ctcggtggtg aacattcgaa cgctcgaaaa agtcagtgcc gaagcaggct
     3781 ctggctcgcc cggcgaaaag gagatgcagg agttttttcg tcgttttggc ttgcccatgc
     3841 ccaatcttcc gaggtctggt ccacggcgtg gtcagccgca ggacgaagaa cagccacgcg
     3901 gcgttggctc gggctttgta ctcaccgcgg acggcatgat tctgaccaat gcccatgtgc
     3961 tcgaaggtgc cgatgaggtc atggtgaccc tgaccgacaa acgcgagttc aaggccagaa
     4021 tcattggtgc cgacaaacgc agcgatgtgg cggtggtcaa gatcgatgcc accggcttgc
     4081 ctgcagtcaa ggtgggcgac gtaggccgac tcaaggtcgg tgaatgggtg atggcaattg
     4141 gttcgccttt tggcctggag aacaccgtca ccgccgggat tgtgagcgcg aagcagcgtg
     4201 acaccggtga ctatttgccg ttcattcaga ccgacgtggc cattaatccg ggcaactcgg
     4261 gcgggccact gatcaatatg cgcggcgagg tggtgggcat caacagtcag atttactcac
     4321 gttcaggcgg ttcgatgggt atttcattcg cgattccgat ggacgaggca ttgcgcgtca
     4381 gtgaacaatt gcgcgccagt ggccgtgtca cgcgtgggcg catcggcgtg cagattggtc
     4441 cggtcagcaa ggatgtggcc gaatccattg ggctcggcaa gccccagggt gcgctggtga
     4501 atggggtcga agccggcgcg ccagccgaca aggccggtgt tgaggcgggc gacatcatca
     4561 tcaagtttga cggtaaggcg gtggaaaaat ccagcgacct gccgcgcatg gtcggcagca
     4621 ccaagccggg caccaagagc accctgacgg tatttcgtcg gggcagcacc aaggagctca
     4681 gtgtcgtgat tgcggagatc gaagcggaaa agtcagccaa aaagacaacc gaaaaggacg
     4741 ataagccgaa gcacccgcat attggccaat ccattggttt ggccgtcagt gagctaacca
     4801 aggagcaaaa gggtgccctg tcgggccatg gtggtgtgcg ggttgattca gcggtagaca
     4861 atgcggcgcg cgccggtatc cgtgaaggcg acatcatcat gcagcttggc aacctggtgg
     4921 tctcggacat caagtcattt gaggcggcac tggcgaaagt tgataagacc aagcccattg
     4981 gcgtgctggt gcggcgtggt gaattggcgc agattgtggt gattcgcccc aacaaatagc
     5041 gctgggcagt tcaaccacgc aatccgcaat aggccgcttg tattcctcgt taattcttat
     5101 gagcatttac ttttctggct tcgatggggt tgacccatcg ggtcacgccg cagcgcaccc
     5161 gattgggccg ctgtcaaatc gcctgcgggc gttacgcctg cgtcgtccgc caggacgaca
     5221 tgcgctcagt cgcctgcttc gtcgcccctt gcggcttgta gggacgggca gggcgcagcc
     5281 atgccacatg gctggcctgc gtcaagccgt ccaaacctgc gcaggcacgt ttggagccgc
     5341 ggccatcgcg ctgcctggcc gcttccctag cggcccactc gggcgcgtcc aactgaggaa
     5401 tccaggttga gttggcacag cgttgctgcc gaccacctgg taataacccc aatgccaatg
     5461 cgtttgtgcc aggcaggcag tccaaaccgg agtgcttggg gtttttgcct acaatgcggc
     5521 tccggctttc acatttgcca acgatggttg gtccagggcg cgtcaacttt gaccgcgccc
     5581 tttttctgtt taccggcctt gttccacttt ttcggtcccc ccctggatga atcacatcag
     5641 aaatttttcg atcattgccc acattgatca tggcaaatcg acgctggctg accggctgat
     5701 ccagcgctgt ggcggcttgc aggaacgcga gatgcaggcg caagtgctcg attcgatgga
     5761 cattgaaaaa gaacgaggca tcaccatcaa ggcgcaaacc gctgcgctgc gctaccaggc
     5821 caaggacggc cagatctaca acctcaatct gatcgatacc cccggccatg tggatttcag
     5881 ttatgaggtc agccgctcgt tgtcggcttg tgaaggggcg ttgctggtgg ttgatgccag
     5941 ccagggcgtg gaagcacaaa ccgtggccaa ctgctatacc gcactcgacc tgggtgtcga
     6001 ggtggttccg gtgctcaaca agatggactt gcccaacgcc gacccggaca acgccaggtc
     6061 ggaaattgaa gacgtgattg gcattgatgc cgtcaacgca attgcttgtt cagccaagac
     6121 cggcatgggc atcgaggaca ttctggaggc ggtggtggcc cgcattccgg cacccaaggg
     6181 caaccctgcc gggccgctgc gcgccatgat catcgacagt tggtttgact cctatgtcgg
     6241 tgtggtgatg ctggtgcggg tggttgatgg ccgtctggcc aagggtgagc gcatcaagat
     6301 gatggccacc gagaccactt acaacgccga cggactgggt gtgttcaccc cggccaatga
     6361 gccgcgtgaa gcgctcaatg caggtgaagt gggttatgtc attgccggca ttcgagagtt
     6421 gcaggctgcc aaggtggggg acaccatcac ccttatcaag ccaggcaccg gtggtctggc
     6481 ttttacggcc accgaaccgt tgccgggttt caaggaaatc cagccgcagg tgttcgccgg
     6541 cctgtatccg accgaggcta accagtacga aggcctgcgt gacagcctgg aaaagctcaa
     6601 gctcaatgac gcctccttgc attacgagcc tgaagtttcg caagctctgg gttttggttt
     6661 caggtgcggc ttcttgggtc tgctgcacat ggaaattgtg caggagcggc tcgagcgtga
     6721 gtttgatcag gacttgatca ccactgcccc gagcgtggtc tatcaggtgg tgcaggccga
     6781 cggtgccatc aagatggtcg agaacccgtc caaaatgccg gatcaaggcc gactcgatga
     6841 gattcgggag cctattgtga ccgtgcattt gtacatgcca caggagtacg ttggcgcagt
     6901 gatgacgctg tccaaccaga agcgcggcgt gcagatgaac atggcctaca agggccgcca
     6961 ggtggtgctg acctacgaga tgccgctggc cgagatcgtg atggattttt ttgacaagct
     7021 caagagtgtc tcgcgtggct acgcctcgat ggattacgag ttcaaggaat accgcagcgc
     7081 cgatgtcgtc aaggttgaca ttttgctcaa cggtgaaaag gttgacgcgc tgtcgatcat
     7141 cgtgcatcgc gcgcagtccc agtaccgtgg ccgccaggtg gtggccaaaa tgcgcgaagt
     7201 catttcacgc cagatgtacg acgtcgccat ccaggccgcc atcggatcca acgtgatcgc
     7261 ccgtgaaacc atcaaggcgc tgcgcaagaa cgtcatcgcc aagtgttatg gcggtgatat
     7321 ttcgcgcaag cgcaaactgc ttgaaaaaca gaaggaaggc aagaaacgca tgaaacagat
     7381 tggttcggtc gaggtgcccc aagaggcttt cctggccatt ttgaaggtgg acgcatgatg
     7441 caggcagtca cctctgtcgt actgggagcc tttgtggctt atatcggtgc ctggtatttt
     7501 ggccagattg aaggcaactt tgccctgctg ctgtttttgg ccaccgtggt gactggcgtc
     7561 tattggctgg cagagcggtt ctatttcttg ccacaacgcc agcaagcggc ggccacgctt
     7621 gaaacccagg cactcaagcg caatgaagag ttagggcgga tgggcattgc caaggtcgat
     7681 ggtgatgtgg cgcaggccaa gcgggatatc ctcgcgcagc cctggtggct cgattggacg
     7741 gccgggctgt tcccggtgat cattgcggtg tttgtgctgc gctcgttttt gtttgagccg
     7801 ttcaagattc cgtcgggctc aatgatgccg accctgctgg ttggcgactt gattttggtc
     7861 aataagtacc attatgggct gcgtctgccg gtgctcaaca cccggatcac cgagggtacc
     7921 gcgccgcagc gtggtgacgt gatggtgttt cgctacccgc ccaagcccag tctggactac
     7981 atcaagcggg tcgtcggtgt gccgggggat gaggttgcgt acctcaacaa gcgcttgacc
     8041 atcaacggca aagtgcagga cacccaggcg ctgccagagt tctttgatga agacaccatg
     8101 cgctacttca agcaatttga tgaggccctg ggtgacaaaa aacatcgcgt gctcaatgac
     8161 gaaaaccgac cggcgtttgt gcccggggcg gaccaatttg agttcaagac caattgtcgc
     8221 tacagtgttg agggcgtggt gtgcaaagtg cctgcaggtc actatttcat gatgggcgac
     8281 aaccgcgaca attcgctgga ctcgcgctat tggggttttg tgcccgacaa aaacattgtt
     8341 ggcaaggcat tttttgtctg gatgaacttc ggcaacgtca agcgtatcgg cgcattcaac
     8401 tgatcaggag aatttcatgg cgacacattt cagatccagt caacgtggcc tgtcctttat
     8461 cggactggtg atgatagcgg tggtggttgc aagcacgggc ctggttttgg cgcagatggt
     8521 gccaacttac atcgaattcc tggcggttgg caaggcggcc aagaaggccg ctagcgggac
     8581 caccatcgtc gatatccgca acacctttga caaagctgcc agcatcgaca acatcagcgc
     8641 catcagcggc aaagaccttg acatctccaa gcagggtgac aaggtggtgg tcgcctttgc
     8701 ctatcagcgc gaaattcacc tggccggtcc ggtctatctg ctgatgaagt atgaaggcaa
     8761 atcgcaataa cgtgaaagca caggtgatgg tttgaatgcc gacctgcacc acttgcaaaa
     8821 gcgtttgcag catgagtttg ttgacttcaa tttgctgacg ctggctgtca cgcaccgcag
     8881 tttttctatt gaccacaatg agcgccttga gtttttgggc gattcggtgc tgggcttgtg
     8941 cgtggctgat ctgctctatg aacgtttgag cgcattgcct gagggcgatt tatcgcgggt
     9001 ccgcgccaat ctggttcggc aagaaacctt gcatcaactg gccgtcacgc tgggtttgcc
     9061 ggacgtgatc cgcctcggtg aaggtgaaat gcgctctggt ggccagaagc ggccatccat
     9121 tctggccgat gcgctggaag ccctgatcgg tgcggtgtac ctcgacggcg ggtttgccgc
     9181 tgccaagagc ttggtgcgac tgctttacca ggatgttgaa gtcaacccct tgatggcggc
     9241 gattggcaaa gatccgaaaa ccgagctgca ggaatggctg cagggtcggc gcttgaagct
     9301 gccggcctac aaggtggtgg ctacgctagg ctcggcgcac cagcagacct ttgatgtcga
     9361 gtgcgagatt gccgaactca agctgtccga gcgcggcatt gggggctcgc gtcgctcggg
     9421 tgaacaggct gcagcgaccg ccatgctgca aactctcaag tcgctaggaa aaccatgaac
     9481 actacaaaac caatagctga tagcgaagac gggcagggcg ctcaaggcca aaatgacgtg
     9541 gaagcattgc tggcaggcct gcgtcgcgac atgcgcccgg ctggtcccgc gcaaagcccg
     9601 ctggaaggcc agcgttgtgg actgattgcc attgtgggta agcccaacgt gggcaagtcg
     9661 accctgctca acgcgctggt tgggcaaaaa atcagcatca cctcgcgcaa ggcgcaaacc
     9721 acgcggcacc gcatcactgg cattcacacg accggtgcga cgcagtttgt gtttgtcgat
     9781 acaccaggtt ttcaaaccct gcacggcaac gcgttgaaca agtcattgaa caagaccgtg
     9841 gtcggtgcgg tggctgatgt cgatttgatt ttgttcatcg tggaggctgg cagctttacc
     9901 ccggccgatg cgcgcgtgct ggcgttgctg ggcaagcaaa taccgacact gctgattgcc
     9961 aacaagcttg accacgtgca ccgccgtggc gacatagcac cctggctgca aaccatggcg
    10021 accaaacatg cgtttgccga attcgtgccg atgtcggcca agaacagcaa agacgtggaa
    10081 cggctgctcg gcatttgcga aaaatacctg cccgaacagg cctggtggta tgcggccgac
    10141 gagctgaccg accgcagtga gcgctttatg gccgccgaga tggtgcgcga aaagctgttt
    10201 cgcctgaccg gcgacgagtt gccttacacc tcgaccgtgg tgatcgacaa gtttgaggaa
    10261 gaagctggtc gcggcaaaca aaagcgcctg ctgaaaattg ccgccaccat tgtggttgag
    10321 cgcgacagcc acaaggcgat ggtgattggc gacaaaggag aacgcgtcaa gcgcattggc
    10381 atggagaccc gggttgagct ggaaaagctg atggatgcca aggtgttcat tgaaatgtgg
    10441 gtcaaggtgc gctcgggctg ggccgatgat gaggcacggg ttcggtcatt tggctacgaa
    10501 taggtggtac accgggctgt agtcctgacc ttgccgtgcg ggtatgcctt cgatttattg
    10561 ctgaaaagag ctggctggaa atcggccagg ggagcaactg gcatt
//
LOCUS       DAOSLL010000077        10576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6152_length_10576_cov_3.884612, whole genome shotgun sequence.
ACCESSION   DAOSLL010000077 DAOSLL010000000
VERSION     DAOSLL010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10576
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6152_length_10576_cov_3.884612"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="PLB25_05005"
     CDS             <1..398
                     /locus_tag="PLB25_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40974.1"
                     /translation="NFEPLKTSVYINQWVTKQRILEEALLRGTYVKDRLTGRDWAAAC
                     CGASREPTTTQAGLGALALIAPLSRPGVLPCNFGTVSASGFTGLGSTIAGQCEAMQRQ
                     RDAPLTLAQNRPPALASHALKAINCIAIR"
     gene            complement(400..1404)
                     /locus_tag="PLB25_05010"
     CDS             complement(400..1404)
                     /locus_tag="PLB25_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HOE40975.1"
                     /translation="MKKIIVLGGTGFVGAHVCEKLVREGWEVTLPTRRRANADHVKHL
                     PNLTVLEQDIHDDLTLNHVVAGHDALVNLVAILHGTQEAFERVHVALPRKIAHACAAG
                     GVRQVVHLSALGADALQPERAPSMYLRSKGEGEAVLMQAATGAGAGLAAQVCFDLSIL
                     RPSVIFGANDKFINLFAKLQQVLPLLPLAGASAKFQPVWVEDVASAVLRCLAGASAAV
                     SPRIIEVAGPEVFTLKQLAQLAAQLSGIAGGFGRPVIALPQWAARLQATLMGLAPGAP
                     MMSLDNLDSMKVDNVASGKLPGLASLGLIASALQPIAQDYLTQNSISAGLMGLRRRSH
                     "
     gene            complement(1495..2562)
                     /gene="murB"
                     /locus_tag="PLB25_05015"
     CDS             complement(1495..2562)
                     /gene="murB"
                     /locus_tag="PLB25_05015"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HOE40976.1"
                     /translation="MLVQKNVPLQPFNTFRIVARADALACITSESDVRELLADPKWAA
                     EPKFVLGGGSNIVLTGDVRPLVLKVEIKGRRLLQETARSVLLEASAGEDWHDFVVWTL
                     AQGYHGLENLALIPGTVGASPVQNIGAYGVELQDRFDSLDAINLQTGQVFTLNAAQCG
                     FGYRDSIFKHASTADAGQQAMGLKDRALILRVRFGLPKPWKPVLGYADIAKKMAECDC
                     AEPSAQQLFDWVCEIRRAKMPDPAVLGNAGSFFKNPTVTPEQCADIIARDPKLVHYPL
                     VDGSIKLAAGWLIDACGWRGKSVGHAGVYEKQALVLVNRGSGQNGDGATGGEVMTLAR
                     AIQTSVYERFGILLEPEPVVV"
     gene            2656..3141
                     /locus_tag="PLB25_05020"
     CDS             2656..3141
                     /locus_tag="PLB25_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HOE40977.1"
                     /translation="MPSFDTVCEASIVDVKHAVENTTKEITTRFDFKGTPAAIELKDK
                     EITLIGDADFQLSQIEDILRNKLTKRSVDVRFLDKGEVQKMGGDKVKQVIKVRDGIET
                     ELGKKIQRLIKDSKLKVQAAIQESKLRVTGAKRDDLQAAMALIKKEIADVPLSFDNFR
                     D"
     gene            3171..4127
                     /locus_tag="PLB25_05025"
     CDS             3171..4127
                     /locus_tag="PLB25_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HOE40978.1"
                     /translation="MALIVQTEDEYEDLLGLWSDLESGLGLVLSHPDSTQEFKQRVTQ
                     YDRWMQDLLEHDADVGLYLLFQLAINSPVGYSASHALVSAVLCHLISVDFGLPKHERD
                     SLVCAALTMNIAMTELQDQLATQPGRPNQEQTVAIRAHAAKGAMMLANLGITDELWLE
                     TIQLHHKDDVPGEDLTALTPPHRLAHILHVVDRYAAMISPRQSREGRSAAESAQNIIR
                     GAAEQESGVGQSLIRIVGLCPPGTFVQLDDEEVAVVTRRSTQPNLPDVAIVIDQNGEL
                     MRPPTLHRTASGKPVISSALVAAAIQERINHHIILQLGTQAA"
     gene            4208..4711
                     /locus_tag="PLB25_05030"
     CDS             4208..4711
                     /locus_tag="PLB25_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HOE40979.1"
                     /translation="MTRPIDLPHQSRLTLALRHLLDTQRIATLGTLSAEGKPFVSMVP
                     FAIDPQAGCLVIHVSLLAPHTRNLLNAPQVSMLVMQAEVAGQPVHALPRVSLDGTAAR
                     LLPDSPAWQTARSAYLGRFPEAVPMTELGDFSFVAVTVTQARQVAGFGAARALDADEI
                     ASLLRPL"
     gene            complement(4775..5479)
                     /locus_tag="PLB25_05035"
     CDS             complement(4775..5479)
                     /locus_tag="PLB25_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidotransferase"
                     /protein_id="HOE40980.1"
                     /translation="MKLCILENDYLDPAIEATYIGYGAMFERLLRDAGATGEFDIFNT
                     LRGHYPASFDGYNAVLLTGSRADCFSQEPWVLRLRQQVEMLLQAKKKLIGVCFGHQLI
                     ALCLGAEVGRAPQGWGAGRMQYQWLAPDLPQAQGRSSIALLASHQDQVFDLPANARLL
                     ASSEFCPVAAFALDEQVFCVQAHPEFVEDYSAFLLNKRRATLGEEKYRACMQGLAHGH
                     EGLAVARMMVAFVEGR"
     gene            complement(5476..6207)
                     /gene="plsY"
                     /locus_tag="PLB25_05040"
     CDS             complement(5476..6207)
                     /gene="plsY"
                     /locus_tag="PLB25_05040"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HOE40981.1"
                     /translation="MQSLFPILAAAAGYLVGSLSFAVIVSRAMGLDDPRTFGSKNPGA
                     TNVLRSGSKAAAIVTLLLDAAKGWLPVMLVRWFGKPYGLEDGTVAMVGLAAFLGHLYP
                     VFFRFAGGKRVATALGVLLGLNGWLGLAGMLIWLATALLFRYSSLASIVVALLAPAVY
                     ALGGGDLWLVSKSIALAVVLMSLLLLYRHAENINRLIKGTESKLGKKPGVQAHPPVQD
                     QHAHHGHQTGRKAQRSHQRKGDHNV"
     gene            complement(6275..6772)
                     /locus_tag="PLB25_05045"
     CDS             complement(6275..6772)
                     /locus_tag="PLB25_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HOE40982.1"
                     /translation="MAKKDHVSETPATQRLKAEKVVFTEHPYEYLAHGGAQHSALVLG
                     WDPFHVVKTLIMQDQDAKPLVVLMHGNRKVSTKNLARQIGAKAVEPCAPEVASRHSGY
                     LVGGTSPFGLRRAMPVFIEASILDLPKILINGGRRGYLVGLDPQLCVRLLGARPVQCA
                     LSLSD"
     gene            6942..7316
                     /locus_tag="PLB25_05050"
     CDS             6942..7316
                     /locus_tag="PLB25_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40983.1"
                     /translation="MRKHVESQSALTSHHSAPLAWRSRCALALLASLVAAGNAVAAPC
                     FTVDATRVTVNQLFATGVGSHLVDLEDMPSVSTMVSRGAESHNLVFMNIKRDAAKKNV
                     TAFAAKIDDKAYEFPKDSCSKK"
     gene            7865..8977
                     /gene="ribD"
                     /locus_tag="PLB25_05055"
     CDS             7865..8977
                     /gene="ribD"
                     /locus_tag="PLB25_05055"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HOE40984.1"
                     /translation="MHRTHLAKALDLAAQCLTLTSPNPHVGCVLAGPYGNILGQGHTQ
                     RAGGHHAEIMALREAQSRHQSVRGATAYVTLEPCAHHGRTAPCCDALIEAGISKVIAT
                     HTDPNPLVAGQGFERLREAGVQVEVLPSNDPLAVQSREINIGFFSRMIRNTPWVRMKI
                     AASLDGRTALDNGISQWITSDAARADGHAWRARSCAVLTGIGTVLADNPKLDVRLLPT
                     RRQPGLVVVDSHLKTPLDAHLFLAKRALWIWAAGQNEAKKAALEARGARVLHRPDADG
                     RVDLAGMLQDLAECEINELHVEAGHKLNGSLIRAGLVDEYLIYLAPKMLGQGLGMTNL
                     GPFTQLSDALELRFHNVEPVGADLRIVARAPGREAF"
     gene            9010..9636
                     /locus_tag="PLB25_05060"
     CDS             9010..9636
                     /locus_tag="PLB25_05060"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HOE40985.1"
                     /translation="MFTGIITGLGRIAAVLPLGDSASHGKRLTLVGPPGYLSDISPGD
                     SIALNGACMTVTTLGPEADSFTVDISAESLDKTAGLETLGPVNLEKALRANDRLGGHL
                     VSGHVDGIGTVSRFEQVGESWTLQLLAPKSMGKYLAYKGSITVNGVSLTVNSVEDLAA
                     GCQISINLIPHTVQNTSLGLLRSGSRVNLEIDLIARYVERMLSADSGA"
     gene            complement(9809..10054)
                     /locus_tag="PLB25_05065"
     CDS             complement(9809..10054)
                     /locus_tag="PLB25_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE40986.1"
                     /translation="MIAEGIKAVERIVVDGLQKVRPGMVAAVFGDTPSAIASRAAKLR
                     DTGTETEQAQTVLDQRSAVIQLCLSLASGGHESDMPI"
BASE COUNT         2223 a         3361 c         3027 g         1965 t
ORIGIN      
        1 gcaatttcga gccactcaaa accagcgtct atataaatca atgggttaca aagcagcgaa
       61 tcctggaaga agcgttatta cgcggaactt acgttaaaga ccggttaaca ggtcgggact
      121 gggccgcagc gtgttgtggc gcatcgcgcg agccgacaac gacacaagcc gggctcggcg
      181 cgctcgccct tatcgcaccg ttatcgcgcc ctggcgtact gccttgcaat ttcggcacag
      241 tgtcggcatc cggattcact ggccttggca gcaccatcgc tggccagtgc gaggccatgc
      301 aacggcagcg cgatgcaccg ctgactcttg cacaaaatcg ccctccagcc cttgccagtc
      361 atgcgttgaa agctattaac tgtatagcaa tccggtgatt cagtgggagc gccgacgcag
      421 gcccatcaag ccagcgctga tgctgttctg tgtcaggtag tcctgcgcaa ttggctgcaa
      481 ggctgaggcg atcaggccca aagatgccag cccgggcagc ttgcccgatg ccacgttgtc
      541 caccttcatc gagtccaggt tgtccagact catcatcggc gcacctggtg ccagccccat
      601 caaggtggcc tgcaagcgcg cggcccattg cggcaaggca atcaccggcc gcccgaatcc
      661 gccggcaatg ccgctgagct gcgccgccag ttgcgccagt tgcttgaggg taaacacctc
      721 tggcccagca acttcaatga tgcgcggcga gactgcagca ctggccccgg ccaggcatcg
      781 cagcacggcg ctggccacgt cttcgaccca caccggctga aatttggccg acgcgccagc
      841 cagcggcagc aacggcaaaa cctgctgcaa cttggcaaac aggttgatga acttgtcatt
      901 ggcaccaaaa atcacgctgg gccgcagaat gctcaggtca aaacacacct gcgccgccag
      961 accggccccg gccccggtgg cggcctgcat cagtaccgcc tcaccctcgc ccttggagcg
     1021 caaatacatc gacggggcgc gctcgggctg caaggcatcg gcacccagcg cgctgaggtg
     1081 caccacttgc ctgacaccgc cggccgcaca ggcgtgggca atcttgcgcg gcagcgccac
     1141 atgtacccgc tcaaacgcct cttgcgtgcc atgcaagatg gcgaccaggt tcaccagcgc
     1201 atcgtgcccg gccaccacgt ggttcaaggt caggtcgtca tggatgtctt gttccagtac
     1261 ggtcaggttt ggcaaatgtt tgacatgatc agcattggca cgacgacgcg ttggcagggt
     1321 tacttcccaa ccttctcgca ccagtttttc acacacatgg gcacctacaa agccggtacc
     1381 accaagcaca atgatttttt tcatgggcgc atcatgcttg aaaaagctgg tttcaaaagc
     1441 cgcgcggtca agaaatgcca caaatgagcg ggtttcatcc gcgtcaaagg ccattcatac
     1501 aacgactggc tctggctcaa gcaagatgcc gaagcgctca tacacgctgg tctggatcgc
     1561 ccgggccagg gtcatgacct cgccgccagt ggcaccatcg ccattctgac cactgccacg
     1621 gttgaccagc accaacgcct gtttttcata aaccccggca tgcccgactg atttgccgcg
     1681 ccagccgcag gcatcgatga gccagccggc ggccagcttg atcgagccat cgaccagcgg
     1741 gtaatgcacc agcttggggt cacgggcaat gatgtcggcg cattgctctg gcgtgaccgt
     1801 cgggtttttg aaaaaactgc cagcattgcc aagcactgcc gggtcgggca ttttggcgcg
     1861 gcgaatctcg caaacccagt caaaaagctg ctgcgcggac ggctctgcgc aatcgcattc
     1921 ggccattttt ttcgcaatat cggcataacc cagcaccggc ttccagggtt tgggcagacc
     1981 aaaacgcacg cgcaggatca gcgcgcggtc tttcaagccc atcgcctgct gcccggcatc
     2041 agcggtgctg gcgtgcttga agatcgagtc acggtagcca aagccgcatt gggcggcgtt
     2101 gagggtaaag acctggccag tctgcagatt gatcgcatcg agcgagtcaa agcggtcttg
     2161 taattcgaca ccataggcac cgatgttttg caccggcgaa gcgcccacgg tgcccggaat
     2221 caaggccagg ttttccaggc cgtggtagcc ctgtgccagc gtccagacga caaaatcatg
     2281 ccagtcttcg ccggcactgg cttcgagcaa aacgcttcgg gcggtttcct gcagcaggcg
     2341 gcgacccttt atttcaactt tcaagaccaa tggcctgaca tcgccggtca gcacgatgtt
     2401 gctgccgcca cccagcacaa atttgggctc ggccgcccac ttcgggtcag ccagcagctc
     2461 acgcacatcg gattcgctgg taatgcacgc cagcgcatcg gcccgggcga cgatacgaaa
     2521 ggtgttgaaa ggctgcaggg gcacgttttt ctgaactaac atggcggcat tgtcgcctga
     2581 ccggtacggg ccaaaaagac cacctgcaaa cccgtgtgac cggcacgctt tgccttcaac
     2641 cctgatccga aaaaaatgcc ttcttttgac actgtttgcg aagccagcat cgtcgatgtc
     2701 aagcacgccg tagaaaacac caccaaggaa atcaccaccc gctttgactt caagggcacg
     2761 ccagcagcga ttgaactcaa ggacaaggaa atcaccctga ttggcgatgc cgacttccag
     2821 ctcagccaga tcgaagatat cttgcgcaac aagctgacca agcgcagcgt ggatgtgcgc
     2881 tttctcgaca agggcgaggt gcagaagatg ggtggcgaca aggtcaaaca ggtcatcaag
     2941 gtacgcgacg gcatcgagac cgagttgggc aaaaagattc agcgactcat caaggacagc
     3001 aaactcaagg ttcaggccgc cattcaggaa agcaagctgc gggtcaccgg tgccaagcgc
     3061 gacgacctgc aggccgccat ggccctgatc aaaaaagaga ttgccgacgt gcccctgagt
     3121 tttgataatt ttcgggatta aggccgcaca ccaacacagc cacaccaacc atggccctga
     3181 ttgtccaaac cgaagacgaa tacgaagacc tgctggggct ttggtctgac ctggagtccg
     3241 gtctggggct ggtgttaagc caccccgaca gcacgcaaga attcaaacag cgggttaccc
     3301 agtacgaccg ctggatgcag gacctgctgg agcacgatgc cgatgtcggc ctgtatctgc
     3361 tgtttcaact ggcgatcaat tccccggtgg gctacagcgc ctcgcatgcg ctggtcagcg
     3421 cggtgctgtg tcatctgatt tcggttgact tcggcctgcc aaagcacgag cgcgacagcc
     3481 tggtctgtgc cgcgctgacg atgaacattg ccatgaccga actgcaggac cagttggcaa
     3541 cccagccagg tcgccccaac caggaacaaa ccgttgccat tcgcgcccat gctgccaaag
     3601 gtgccatgat gctggcgaac ctgggcatca ccgatgagct gtggctggag accatccaac
     3661 tccatcacaa ggacgatgtc ccaggggaag acctgactgc gctgacacca ccacaccggc
     3721 tggcgcacat cctgcacgtg gtcgaccgct acgccgcgat gatcagcccg cgccaatcgc
     3781 gcgagggccg cagtgccgcc gaatcggcgc aaaacatcat tcgtggtgcc gccgagcagg
     3841 aaagcggtgt cggtcaatcc ctgatccgca tcgttggctt atgcccgcct ggcaccttcg
     3901 tgcagctcga tgatgaagag gtggcggtgg tgacccgccg cagcacccag cccaacctgc
     3961 cggatgtggc gatcgtgatc gatcaaaatg gcgaactgat gcgcccgccc accctgcacc
     4021 gaaccgccag tggcaagcca gtcatcagct cggcgctggt ggccgccgcc atacaggaac
     4081 ggatcaacca ccacatcatc ctgcaactcg gcacgcaggc cgcatgagga cacctcgaat
     4141 actctctttt atgtagcttg atgcgcatgc tccataaggg attggagcga atttgactct
     4201 gaaaaacatg accagaccga ttgacctgcc gcaccagagc cgcctgaccc tggcgctgcg
     4261 tcatttgctc gatacccagc gcattgcgac gctgggcacc ctgagcgccg aaggcaagcc
     4321 atttgtttcg atggtgccgt ttgcaattga cccgcaagcc ggttgtttgg tgattcatgt
     4381 cagcttgctg gcaccacaca cccgcaacct gctcaacgcg ccgcaggtgt cgatgctggt
     4441 gatgcaagcc gaagtggctg gtcaaccggt acatgccctg ccacgggtgt cactggacgg
     4501 cactgccgcc cgtttgctgc cagacagccc ggcctggcaa actgcccgca gtgcatatct
     4561 ggggcgtttt ccagaggcag tgccaatgac cgaactgggc gacttcagct ttgttgccgt
     4621 cacggtgacg caggcgcggc aggtggccgg ctttggcgct gcgcgggcgc tcgatgccga
     4681 tgaaatagcc agcctgttgc gcccgctgtg acgctggttg aaaccaggca tgcccagctc
     4741 gggcacgggt ccagtcgcgc ggctggggca aggctcaccg gccctcgaca aacgcgacca
     4801 tcatgcgcgc cactgccagc ccctcgtgac cgtgcgccag gccctgcatg caggcgcggt
     4861 acttctcttc gcccagcgtg gcgcggcgtt tgttgagtaa aaaggcgctg taatcctcga
     4921 caaattcggg gtgcgcctgg acgcaaaaaa cctgctcgtc cagcgcaaac gcggccaccg
     4981 gacaaaattc gctgctggcc agcaagcgcg cgttggcggg caaatcaaac acctgatcct
     5041 ggtggctggc cagcagggca atgctgctgc ggccctgcgc ctgcggcaag tcgggtgcca
     5101 gccactggta ctgcatgcgc cctgcccccc aaccctgcgg cgcacgcccc acttcggcgc
     5161 ccaggcacag ggcaatcaac tgatgaccaa aacacacgcc aatcaacttc tttttggctt
     5221 gcagcagcat ttcaacctgc tggcgcagcc gcagcaccca gggttcctgt gaaaaacaat
     5281 cggcgcggct accggtcagc agcaccgcgt tgtagccatc aaacgaagcc gggtagtgac
     5341 cccgcaaggt gttgaaaatg tcaaactcgc cagttgcacc ggcatcgcgc agcaggcgct
     5401 caaacatggc accatagcca atgtaggtgg cctcaatcgc cggatcaagg tagtcgtttt
     5461 caagaatgca gagtttcata cgttgtggtc gcctttgcgt tggtggctgc gctgtgcctt
     5521 gcggccggtt tgatgaccgt ggtgggcatg ctgatcctgc accggcgggt gcgcttgaac
     5581 accgggcttt ttgcccaatt tggactcagt gcccttgatc aagcggttga tgttttcggc
     5641 atggcggtac agcagcagca gcgacatcag caccaccgcc agggcgatgc tcttgctgac
     5701 cagccagagg tcaccgccac ccagcgcata caccgccggt gccagcaagg cgaccacgat
     5761 ggacgccagt gatgaatagc gaaacaacag tgccgtggcc agccagatca gcatgccagc
     5821 cagacccagc cagccgttca gaccaagcag cacgcccaag gcagtggcga ctctcttgcc
     5881 accggcaaac ctgaaaaaca ccgggtacag gtggcccaaa aaggccgcca gaccaaccat
     5941 tgccacggtg ccgtcttcca ggccataggg cttgccaaac cagcgcacca gcatcaccgg
     6001 cagccagcct ttggccgcat cgagcagcag cgtgacgatg gcggcggctt tgctaccgga
     6061 ccgcagcaca ttggtggcac ccggattttt gctgccaaaa gtgcgcgggt catccagacc
     6121 catggcgcgg ctgacaatca ccgcaaacga caacgagccc accagatagc cagccgcagc
     6181 cgccaaaatg ggaaaaagac tttgcatgga ttccaaaata actcctgttg gcctgcgaca
     6241 ccgatcaagg tctattttgc cagcctgggc aggctcagtc ggaaaggctc aaggcgcact
     6301 gcaccggcct ggccccgagc aagcgcacgc acaactgcgg gtcaagcccc accaggtagc
     6361 cacgccgccc gccgttgatc aggatcttgg gcaaatccag aatgctcgct tcgatgaaca
     6421 ccggcatggc ccgacgcagg ccaaacggcg aagtgccgcc caccaggtaa ccgctgtgcc
     6481 gactggcgac ctcgggcgca cagggctcga ctgccttggc cccgatctgg cgcgccaggt
     6541 ttttcgtcga caccttgcgg ttgccgtgca tcagcaccac caggggtttg gcatcctggt
     6601 cctgcatgat cagggttttg acgacatgaa acgggtccca acccagcacc aacgcgctgt
     6661 gctgggcccc gccgtgcgcc aggtattcgt aaggatgctc ggtaaaaacc actttttcgg
     6721 ctttcaggcg ttgggtggca ggggtttctg acacatgatc ttttttggcc atggggctat
     6781 tttcctgcca agtgcaggca tcaacgccgc gccgagacaa cgccaactgg ccgaatccgc
     6841 cggtttggtc ggataaactg cgctctttgc attgcccaat gacgcggtga tcaacctcca
     6901 tcatgctgct ttgcccaacc ctttcgaatc aaggaaccaa catgaggaaa cacgttgaat
     6961 cgcaatctgc cttgaccagc caccacagcg ccccactggc atggcgcagc aggtgcgcgc
     7021 tggcgctgct cgcgagtctg gtggcagcgg gcaacgcggt tgccgcacct tgtttcacgg
     7081 ttgatgcaac cagggtcact gtcaaccagc tcttcgccac cggagtgggc agccaccttg
     7141 ttgaccttga ggacatgccc tcggtcagca ccatggtttc gcgcggcgcc gagagccaca
     7201 acctggtctt catgaacatc aaacgcgacg cagccaagaa aaacgtcact gccttcgcgg
     7261 ccaaaattga cgacaaggcg tatgaatttc cgaaggattc ctgttccaaa aaataggcaa
     7321 ctgcgcaccg tctgatggat gcccgcaccc cggtctgtgc caactggcca ttgccgcttg
     7381 acggtaacgc acctgcaatg ccagcccgtg acgcggtctt tgacaggact cttttgctac
     7441 ttttttgata gctggtagcg caatgaccac gggctctaga ggcctaaaac atgcctattt
     7501 ggcagaatct ttcaaccccg cgtgtcttgt ccaagtatcc tcagttgccc gcttgtcttc
     7561 atctgtaagt ccctatgaga atttgctttt cagtttcgat ggcgtacagc ttttgggtgg
     7621 catcgcttcg cacccgattg aaccgctggc aacgcgcctg ggggtgtttc acatccctca
     7681 tccaccatga cgaaatgccc tcggtagccc gcgtcgtcgc cgcccctgca gggcgcagcc
     7741 atgccaaaag gctggcctgc ttgatgcggc ccaaacctgc tcaggccggt ttggagccgc
     7801 ggttttcgcc ctgcccagcg acccacttgg gcgcgtccaa ctgagccatc caggattttt
     7861 gaccatgcat cgaacacacc ttgccaaagc gcttgatctg gcagcccaat gcctgacctt
     7921 gacctcaccc aacccgcatg tcggctgcgt tctggccggg ccttacggca acattctggg
     7981 ccagggccac acccagcgcg cgggcggcca ccacgctgaa atcatggcgc tgcgcgaagc
     8041 ccagtccagg caccagagcg tgcgcggcgc caccgcctat gtcacgctgg agccctgcgc
     8101 acaccatggc cgcactgcgc cctgttgcga cgcgctgatc gaggctggca tctcaaaggt
     8161 catcgccacc catactgatc caaacccgct ggtggcaggc cagggcttcg agcgtctgcg
     8221 cgaagctggc gtacaggtcg aggtgttgcc atccaacgac ccgctggcgg tgcaatcgcg
     8281 cgagatcaac atcggctttt tcagccgcat gatccgcaac acaccctggg tgcgcatgaa
     8341 gatcgcggct tcgctcgacg gcaggacggc gctggacaac gggatcagcc agtggatcac
     8401 ctccgacgca gcacgtgccg acggccacgc ctggcgcgcc cgcagttgcg ccgtgctgac
     8461 cggcatcggc accgtgctgg cagacaaccc caagcttgac gtgcgcctgc tgccaacccg
     8521 gcgtcagccc ggtctggtgg tggtggacag tcacctcaaa acgcctcttg atgctcatct
     8581 ttttctagct aaacgcgcgc tgtggatttg ggctgcaggc caaaatgagg ctaaaaaggc
     8641 agcactcgaa gcacgtggcg cccgggtcct gcaccggccc gatgctgacg gccgggttga
     8701 tctggctggc atgctgcaag acctggctga gtgcgaaatc aacgaactgc acgtcgaggc
     8761 cggccacaag ctcaatggct cgctgattcg cgccgggctg gttgatgagt acttgattta
     8821 cctcgccccc aaaatgctgg ggcaaggcct ggggatgacc aacctggggc cgttcacgca
     8881 gctttcggac gcgctggaac tgcgctttca caatgtcgag ccagtcggtg ccgacttgcg
     8941 tatcgtggcc agagcgcctg ggcgcgaggc tttttgagac catccatgcc agtctgggag
     9001 aatctgacca tgtttacagg aatcatcacc ggccttggcc gcatcgctgc cgttttacct
     9061 ttgggcgact ctgccagcca cggcaaacgc ctgaccctgg ttggcccgcc gggctacctg
     9121 tccgatatca gcccgggcga cagcattgcg ctcaacgggg cctgcatgac ggtcactacg
     9181 cttggccccg aggccgacag cttcaccgtg gacatctcgg ccgagtcgct tgacaagacg
     9241 gccgggcttg aaaccctggg gccggtcaac ctggaaaagg cgctgcgcgc caatgatcgc
     9301 ctgggcgggc acctggtgtc gggtcatgtc gatggcattg gcaccgtgag ccgctttgaa
     9361 caggtgggcg aaagctggac gctgcagttg ctggccccaa agtcgatggg caaatacctt
     9421 gcctacaaag gatcgatcac cgtcaacggg gtcagcctga ccgtcaacag cgtcgaagac
     9481 ctcgctgcag gctgccagat cagcatcaac ctgatcccgc acaccgtgca aaacacatcg
     9541 ctgggtttgc tcaggagtgg cagccgcgtc aaccttgaga ttgacctgat tgcgcgctat
     9601 gttgagcgca tgctcagtgc ggattctggc gcataagaaa aattgcctgt ttgctggatg
     9661 ccccggttca gcgtctgagt caccagtgct cgccaccagc cattgaccca caggccttca
     9721 gttaaacccc atttccaacg caggaatgga aaaatcgttc acggtgaaac cgaagttcgc
     9781 cgcacttgtc gcacatattg gaccgggctc agatgggcat gtcggactcg tgcccgccgc
     9841 tggccagcga aaggcaaagc tggatcaccg cactgcgctg atccagcacc gtttgcgcct
     9901 gctcggtctc ggtcccggtg tcgcgcaact tggctgcgcg ggatgcgatt gccgacggtg
     9961 tgtcgccaaa cactgctgcc accatgccag ggcgcacctt ttgcagtccg tcaaccacaa
    10021 tgcgctcaac ggccttgatg ccttcggcaa tcacccactg atcgccgacg cagacggtgg
    10081 ctacagtacc gcaaggccaa gcatcatcat gtcggattga cttggaaaag gaaccagacg
    10141 cgacttgcca aaaatcagac ttgggcctgg taccgctggt gtcaccagcc ctggcgaacg
    10201 acggcaatgc gcggcacttg cccgccgagc atcctggatt gcccggttga ccgaaacaag
    10261 gctgggtggc gggcaaagat gtgatcagtg gggtgcaatc gacgaccgcc tttgccccgg
    10321 cgcgaggtcg cagcaaacgc cacacccgcc gagcagtcag tccgtgaggc cgcaacttca
    10381 cccccacctg gtgaaggcca cgagatcgcg cttcgcaagc tcgcgtgcca gcgctgcggc
    10441 ttcacggctg cttgctgtta tcggctccgg tgcagggatg gcttatgccg accaacacgg
    10501 tactggcgta tcaccaacag tgtcagcaag ctgatcaaaa gaagacgtcc ccattcgctg
    10561 agggtgggaa tactcg
//
LOCUS       DAOSLL010000078        10487 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6208_length_10487_cov_4.859663, whole genome shotgun sequence.
ACCESSION   DAOSLL010000078 DAOSLL010000000
VERSION     DAOSLL010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10487)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10487)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10487
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6208_length_10487_cov_4.859663"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..961
                     /locus_tag="PLB25_05070"
     CDS             <1..961
                     /locus_tag="PLB25_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HOE40987.1"
                     /translation="DAASAPPDDEVPPGAGAASPELGLSKSVDPATTGQLNAPSALEP
                     HLLMMTATPIPRTLAMSYYADLDLSTIDELPPGRTPIITKVVNEARRAEVVERIGLQI
                     AQGRQIYWVCPLIEESEALDLTNATETHAQLSAALPGVMVGLLHSRMPAAEKKAVMSL
                     FTGGQMGLLVSTTVIEVGVDVPNASLMVIEHAERFGLSQLHQLRGRVGRGTAASACVL
                     LYGTGDAPRLGQTARERLRAMAESNDGFEIARRDLEIRGPGEFMGARQSGAALLRFAD
                     VVEDATVLAWARKLAPVMLDQYPALAERHVQRWLGTRAEFLKA"
     gene            complement(1008..1376)
                     /locus_tag="PLB25_05075"
     CDS             complement(1008..1376)
                     /locus_tag="PLB25_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOE40988.1"
                     /translation="MLLSYQRVPTSVLSHFKHRASRIVVIFRAKVRQVDGEYTVVAAR
                     LRELALEQVGCLKFQAVNEGKDEVALSYWPNEDSIRAWKSHAEHVLAQQSGSERWYQS
                     YVVQVVQIGRKYRFDGSPGA"
     gene            1378..2331
                     /locus_tag="PLB25_05080"
     CDS             1378..2331
                     /locus_tag="PLB25_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOE40989.1"
                     /translation="MTLTELKYIVAVARERHFGKAAEACYVSQPTLSVAIKKLEEELE
                     VKLFERSANEVTVTHLGEEIVRQAQSVLEQAAAIKEIAKRGKNPLAGPLTLGVIYTIS
                     PYLLPALVRQMIQRIPEMPLMLQENFTVKLLDMLRTGEIDCAILAAPFPDAGLAMAAL
                     YDEPFMAAVPTNHPLAAQADVTSEQLKSETMLLLGSGHCFRDHVLEVCPEFAHFSANA
                     EGIRKSFEGSSLETIKHMVAAGMGVTLVPRLSVPKESFEPAPSRKRDEDPYIKYLPFA
                     GDPPKRRVVLAWRRSFTRYEAIAALRNAVYACELPGVNRLS"
     gene            2371..3273
                     /locus_tag="PLB25_05085"
     CDS             2371..3273
                     /locus_tag="PLB25_05085"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006753361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HOE40990.1"
                     /translation="MSDCQLAVLDDVPNVGRYVYFSIASAVAVDIRQSLARLADMVDG
                     TEVLVGIGPDLVNLLGAHVPGLHGFKALSGATVLVPATPSALCCWLRGDDRGDLLHLT
                     RRLQMALAPGFALDQTVDAFRYGRGPNGHGRDLTGYEDGTENPVADAARAAALVQGAG
                     PWMDGSSFMVVQQWVHDLDAFDALGETRQDHTYGRRRSDNEELADAPASAHVKRTAQE
                     SFTPEAFVLRRSMPWTAGLQSGLMFVAFGHSFAAFEVLMRHMVGLDDGVVDALFGISR
                     PVTGANFWCPPMHHGRLDLRQLGL"
     gene            3283..4149
                     /gene="ubiA"
                     /locus_tag="PLB25_05090"
     CDS             3283..4149
                     /gene="ubiA"
                     /locus_tag="PLB25_05090"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HOE40991.1"
                     /translation="MTTRRSKLDLFLDLIRWNRPAGWLLLLWPTLGALWVAADGFPGW
                     HLLGVFVLGTFLMRSAGCCVNDVADRDYDRHVKRTAQRPVTSGAMSVKQALLVGAVLA
                     LLAFALVLSTNRVTVGMSFAALAITLAYPYAKRFVAMPQAVLGLAFGMGIPMAFAAVL
                     GDVPPLAWVLMLGNLFWVLAYDTEYAMVDRDDDLKIGIKTSAITLGRMDVPVIMLCYL
                     LFIGIWALALAAYAQHAIFKIAICAALLQVAWHFSLIRGRSREGCFKAFRLNHWLGLT
                     VFAGIAASYAVR"
     gene            complement(4164..4979)
                     /gene="proC"
                     /locus_tag="PLB25_05095"
     CDS             complement(4164..4979)
                     /gene="proC"
                     /locus_tag="PLB25_05095"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HOE40992.1"
                     /translation="MTERIAFIGGGNMASAIIGGLIKQGLSPLTIDVVEPFEEARDKL
                     QSSFGITAQVQAGPFLSNAALVIWAVKPQIFRAAAVAVAAHTLQALHLSVAAGIRSDS
                     IAIWLGTDRVVRAMPNTPALVGKGITGLFARAGVSAADQARITQVMASTGESIWLGAE
                     EKLDVVTALSGSGPAYVFYFMEAMTTAGIEMGLSREQAHQLAIATFVGASELARSASE
                     SPQVLRQRVTSKGGTTYAAISSMEENDMRALFIDAMYAARERAKALGDEFGAD"
     gene            complement(5091..6581)
                     /gene="glpK"
                     /locus_tag="PLB25_05100"
     CDS             complement(5091..6581)
                     /gene="glpK"
                     /locus_tag="PLB25_05100"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HOE40993.1"
                     /translation="MTYLLALDQGTSSSRSIVFDANGHIVALAQQELSQIYPQPGWVE
                     HDPMEIWRTQLATARTALAKAGLTARDMRAVGITNQRETTVVWNRKTGLPIHNAIVWQ
                     DRRAEPACLELRERGLAPAIRAKTGLLVDAYFSGTKLKWLLDHVAGARQQAQNGELAF
                     GTVDSWLIWQLTRGAVHATDVSNAARTMLFNVHSNQWDEDLLQALDVPADLLPQVKPS
                     SALYGEVATDLLGHGVTIGGVAGDQQSALFGQACFKAGMAKNTYGTGCFMLMHTGTRF
                     QTSNSGLISTSAAQTTQQPEFAIEGSVFVGGAVVHWLRDGLRAIASSSDVQALAESVP
                     DAGGVMMVPAFTGLGAPYWNPEARGSITGITRGTTLAHIARAALESIAFQSAALLQAM
                     SRDAVADGSAAVAELRVDGGACVNNLLMQFQADLLGIAVIRPAVTETTALGAAYLAGL
                     CTGVFRDTDELSAQWQVERRFEPKMPPGDAADLMARWEHAVRKTVL"
     gene            complement(6634..7401)
                     /locus_tag="PLB25_05105"
     CDS             complement(6634..7401)
                     /locus_tag="PLB25_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOE40994.1"
                     /translation="MNPNPRQLTLLSVVQAQGSVTVEHLADQLGVTLQTVRRDVQRLA
                     DNGLLTRFHGGVRAPGSTVENIAHQQRENLNTAGKLRIARAVAAAVPNHCSLMLNIGT
                     TTECIARALVRHLGLRVITNNLNVATTLSANTNCEVIVVGGVVRGRDRGIVGEAAVDF
                     IRQFKVDIAIIGISGIEPDGSLRDFDYREVKVTQTILTHARQIWLAADSSKFNRPAMV
                     EVGKLSQIDRLFTDAPPPDPFPALLRDAQVDCELAIV"
     gene            7537..8613
                     /locus_tag="PLB25_05110"
     CDS             7537..8613
                     /locus_tag="PLB25_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE40995.1"
                     /translation="MQLTLEGVGKTVGAQTWLHDMSIAPRSSAVTVLLGATQAGKTSL
                     MRIMAGLDVPTCGRVLVDGQNVVGVPVRQRNVAMVYQQFINYPSLKVWDNIASPLTLR
                     GEKNIDQRVRQLAEKLHIDMFLDRYPAELSGGQQQRVALARALAKGAPLMLLDEPLVN
                     LDYKLREELRDELTELFAAGSSTVIYATTEPGEALLLGGHTAVLDAGELLQYGPTAEV
                     FHKPRSLRVARAFSDPPMNLMVADATAAGAVQLVGGPLLSIVLPTTASHKFTLGLRAS
                     ALRIQAMAGDVSLPGKVELAEISGSDTFVHVETLVGDLVAQLTGVHYFELGVQVTIYF
                     SPDQVFVFDDQGLLVLSPARVGGN"
     gene            8615..9691
                     /locus_tag="PLB25_05115"
     CDS             8615..9691
                     /locus_tag="PLB25_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018907390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE40996.1"
                     /translation="MARIELDLAHAYKPGPKQDSDYALLPLQMTFEDGGAYALLGPSG
                     CGKTTLLNIMSGLLAPSQGGVRFDGVDVTRFSPQARNIAQVFQFPVIYDTMTVAENLA
                     FPLRNRQVPKDVINKRVGEIAEVLEMSGQLNQRAANLAADEKQKISLGRGLVRPDVSA
                     VLFDEPLTVIDPHLKWQLRRKIKQIHQELKLTMIYVTHDQVEALTFAQQVVVMTRGRA
                     VQVGSAAQLFERPSHTFVGHFIGSPGMNFLAGQVAQQVLHLADVVLPLHAGLALPSGD
                     IKLGIRPEYVTLASPHAQGALPMTLAHVQDVGTHIMLSASFAGQTVKARLPANLGHLA
                     TGDLIWLKVLGDQTCFYKNEEIVA"
     gene            9688..>10487
                     /locus_tag="PLB25_05120"
     CDS             9688..>10487
                     /locus_tag="PLB25_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE40997.1"
                     /translation="MSVTTKPVNQKAWLLILPVLLCVAFSAILPLMTVVNYSVQDIIS
                     PERRVFVGTEWFAAIMRDEELHGALVRQLTFSLSVLAVELPLGILLALSMPAQGWKSS
                     AVLVVVSLSLLIPWNVVGTIWQIFGRADIGLMGAALQGLGIDYSYTGNAAHAWATVLL
                     MDVWHWTPLVALLGYAGLRSIPDAYYQAASIDGASKFAVFWYVQLPKMRGVLMIAVLL
                     RFMDSFMIYTEPFVLTGGGPGNSTTFLSQFLTQKAVGQFDLGPAAAFS"
BASE COUNT         1900 a         2962 c         3373 g         2252 t
ORIGIN      
        1 tgatgctgcc agtgccccgc cagatgatga ggtgccgccc ggcgctggtg ccgccagccc
       61 cgaactgggg ctctcaaagt ccgttgaccc agccaccacc ggtcagctca atgccccgtc
      121 cgcactggaa ccccatctgc tgatgatgac cgccaccccg attccgcgca cgctggcgat
      181 gagctattac gctgatctgg acctgtccac gattgatgaa ctgccgcccg ggcgcacccc
      241 gatcatcacc aaagtggtca atgaggcgcg ccgtgcagag gtggttgagc gcatcggctt
      301 gcagattgcc caggggcggc agatttattg ggtttgcccg ctgattgaag agagcgaggc
      361 gctagacctg accaatgcca ccgaaaccca cgcgcagttg agcgctgccc tgcccggcgt
      421 gatggtcggc ctgctgcatt cgcgcatgcc cgcggcagag aaaaaggctg tgatgagcct
      481 gtttaccggt ggccagatgg ggctgctggt cagcaccacg gtgattgagg tcggggtcga
      541 tgtgcccaat gcgtcgctga tggtgatcga gcatgccgag cgctttggcc tgtcgcagtt
      601 gcaccagttg cgtggccggg tcgggcgcgg cacggcggct tcggcctgcg tgttgctgta
      661 tggcaccggg gacgcaccgc gcctggggca gacggcgcgg gagcgcctgc gcgccatggc
      721 cgagagcaac gacggttttg agatcgcccg gcgtgacctg gagattcgtg gccccggcga
      781 gttcatgggc gcgcgccagt cgggggccgc gctgctgcgt tttgccgatg tggtggagga
      841 tgcgaccgtg ctggcctggg cgcgcaagct cgcgccggtg atgctcgacc aatacccggc
      901 actggccgaa cggcacgtgc agcgctggct gggcaccagg gcagagtttc tgaaggcctg
      961 acaaaaccga aggcttgtgc cgcaaaagct aaagcagagt gtgccgccta tgcaccgggt
     1021 gagccgtcaa accggtattt gcggccaatc tgcaccacct gcaccacata ggactggtac
     1081 cagcgctcgc ttcccgattg ctgcgccagc acatgctcgg catgcgattt ccaggcgcga
     1141 atgctgtcct cattgggcca ataggacaag gcgacttcgt ctttcccttc gtttacggcc
     1201 tgaaatttca gacacccaac ctgctccagc gccagttcgc gcaggcgcgc agccaccacg
     1261 gtgtactcgc catcgacctg gcggactttg gcgcgaaaaa ttacgacgat cctggaagcc
     1321 ctgtgtttga agtgcgaaag taccgatgtc ggcacgcgct ggtatgacaa tagcaatatg
     1381 actctgaccg aactcaaata catcgtggca gtggcccgcg agcggcactt tggcaaggcc
     1441 gcagaggctt gttacgtgtc ccagccgacc ttgtcggtgg ccatcaaaaa gctggaagaa
     1501 gagcttgagg tgaagctgtt tgaacgcagc gccaacgagg tgacggtgac ccatctgggt
     1561 gaagaaatcg tgcgccaggc acaaagcgtg ctggagcagg cggctgccat caaggaaatt
     1621 gccaagcgtg gcaaaaaccc gttggcaggc ccgctcacgc tgggcgtgat ctacaccatc
     1681 agcccatatc tgttgccagc cctggtgcgc cagatgatcc agcgcatccc ggagatgccg
     1741 ctgatgctgc aagaaaactt caccgtcaag ctgctcgaca tgctgcgcac cggcgaaatt
     1801 gactgcgcca ttctggccgc gccgtttcct gatgccgggc tggcgatggc agcgctgtat
     1861 gacgaaccgt tcatggcggc ggtgccgacc aaccacccgt tggcggcgca ggccgatgtg
     1921 accagcgagc agctcaagtc agagaccatg ctgctgctcg gcagtggtca ttgttttcgc
     1981 gaccacgtgc tggaagtctg ccccgagttt gcccattttt cggccaatgc cgagggcatt
     2041 cgcaaaagct ttgagggctc gtcgctggaa accatcaaac acatggtggc ggctggcatg
     2101 ggtgtcacac tggtgccgcg cctgagcgtg cccaaagagt cgtttgagcc cgcacccagc
     2161 cgcaaacgtg acgaagaccc ctacatcaag tacctgccgt tcgctggcga tccgcccaag
     2221 cgccgggtgg tgctggcctg gcgacgcagt tttacccgct atgaggcgat tgccgcgttg
     2281 cgcaatgccg tctatgcctg tgaattgccg ggcgtgaacc ggttgtcttg aagcagattt
     2341 cactttcaga ttcaacttga aggagcaagc atgtcagatt gtcagttggc ggtgttggac
     2401 gatgttccca atgtcgggcg ctatgtgtat ttttctattg ccagtgcggt ggctgtcgat
     2461 attcggcaaa gcctggcccg gctggcagat atggtcgatg gaaccgaggt gctggttggt
     2521 attgggccgg atctggtcaa tttgctgggc gcgcatgttc cgggtctgca tggcttcaag
     2581 gctctgtcag gtgccaccgt gctggtgccg gcgacaccgt cggccctgtg ctgctggctg
     2641 cgcggcgatg accggggtga tttgctgcat ctgacgcggc gactgcaaat ggcgctggcg
     2701 ccgggctttg cgctcgacca gaccgttgat gcctttcgct atgggcgtgg ccccaatggc
     2761 catgggcggg acctgaccgg ctacgaggac ggcaccgaaa acccggtggc ggatgctgcg
     2821 cgtgccgccg cgctggtgca aggcgcgggc ccgtggatgg atggcagcag cttcatggtg
     2881 gtgcagcagt gggtgcatga cctcgatgca tttgatgcgt tgggtgagac ccggcaggat
     2941 cacacctatg gcaggcgccg cagtgataac gaagaactgg ccgatgcacc cgcgtcggcc
     3001 cacgtcaagc gaaccgcgca agagagcttt acccccgagg cttttgtgct gcgacgctcc
     3061 atgccatgga cggcgggcct gcagtcgggt ctgatgtttg tcgcctttgg ccattcgttt
     3121 gctgcttttg aggtgctgat gcggcacatg gttggcctgg acgacggcgt ggtggatgcc
     3181 ttgtttggca tatccaggcc ggtgaccggt gccaatttct ggtgtccgcc gatgcaccac
     3241 ggccgcctgg atctgcggca actgggtctg taaggggcgc gcgtgacaac tcggcgcagc
     3301 aagcttgacc tgttcctgga tttgattcgc tggaaccggc cggccggctg gttgctgttg
     3361 ctgtggccta cgctgggcgc gttatgggtg gcagccgacg gttttcccgg ctggcatttg
     3421 ctgggcgtgt ttgtgctggg cacttttttg atgcgcagcg ccggttgttg tgtcaatgac
     3481 gtggcagatc gcgactatga ccggcatgtc aagcgcaccg cgcagcggcc agtgaccagc
     3541 ggcgcgatgt cagtcaaaca ggcgctgctg gtcggggcgg tgctggcctt gttggctttt
     3601 gcactggtgc tgagcaccaa ccgggtcacc gtcggcatgt cgtttgcggc gctggcgatc
     3661 accctggcct acccctatgc caagcgtttt gtggcgatgc cgcaggcggt gctggggttg
     3721 gcgtttggca tgggcatccc gatggcgttt gcggcggtgc tgggtgatgt gccgccactg
     3781 gcctgggtgc tgatgctggg caatctgttt tgggtgttgg cctacgacac cgaatacgcc
     3841 atggtggacc gtgacgacga tctgaaaatt ggcatcaaga catcagccat cacgctcggt
     3901 cggatggatg tgccagtgat catgctgtgt tacctgcttt ttataggtat ttgggctcta
     3961 gcccttgctg cgtatgcgca acatgctata tttaaaatag cgatatgtgc ggcgctgctg
     4021 caggtggcgt ggcatttcag cctgattcgc gggcgcagcc gcgagggctg cttcaaggct
     4081 tttcgcctga accactggct tgggctgacc gtgtttgccg gtattgccgc gagttacgcc
     4141 gtcaggtagt caggccaccg ggtctagtcg gcgccaaact cgtcgccaag cgctttggcg
     4201 cgttcgcggg ctgcgtacat ggcatcaatg aacagggcgc gcatgtcgtt ttcttccatg
     4261 gatgaaatcg ccgcgtaagt ggtgccacct ttggatgtga cgcgctggcg cagcacctgg
     4321 ggcgactcgc tggcgctgcg ggccagttcg ctggcaccga caaacgtcgc aattgcgagt
     4381 tggtgcgcct gctcgcggct caggcccatc tcgatgccgg cagtcgtcat cgcctccatg
     4441 aagtaaaaca cgtaagccgg gccggagcct gaaagggctg tcaccacgtc aagtttttct
     4501 tcagcgccca gccagatcga ttcaccggtg ctggccatca cctgggtgat gcgggcctga
     4561 tccgcagcag aaacaccggc acgggcaaac aggccggtga ttcccttgcc caccaatgcc
     4621 ggggtgttgg gcatggcacg cacaacgcgg tcagtaccca gccaaatggc gatgctgtca
     4681 cttcgaatcc cggctgccac gctcaaatgc agcgcctgca aagtgtgggc tgcgactgcc
     4741 acagccgctg ccctgaaaat ttgcggcttg acggcccaga tcaccagcgc tgcattgctt
     4801 agaaacggac ccgcctgcac ctgcgccgtt atgccaaagc tgctttggag cttgtcgcgg
     4861 gcctcttcaa atggttcaac tacgtcgatg gtcaggggtg ataggccctg cttgatcagt
     4921 ccgccaatga tggcgctggc catattgccg ccgccaatga atgcaatgcg ctcggtcatg
     4981 acaggtgcgc agcggtcagg ctgctgctgg gtggtggaaa agcttgagtc taatcaagtt
     5041 tgtggatgca tcagtttgac ctggccaacg caagcgcaga caagccctgc ctaaagcacg
     5101 gttttgcgga ccgcgtgttc ccagcgggcc atcaggtcgg ctgcatcacc tggcggcatt
     5161 ttgggttcga agcggcgctc cacctgccac tgggcgctca gttcatcggt gtccctgaag
     5221 acaccggtgc acaagcctgc caggtaagcc gcgccgagcg ccgtggtttc tgtgactgcg
     5281 ggtcgaatca ccgcaatgcc cagcagatcg gcctgaaatt gcatcagcag gttgttgacg
     5341 caggcaccgc cgtccactcg cagttcggcc actgcggcac tgccgtcggc cactgcatca
     5401 cggctcatcg cctgcagcag ggcggcgctc tgaaatgcga tgctttccag ggccgcgcgg
     5461 gcaatgtggg ccagtgttgt gccacgggtg atgccggtga tgctgccacg cgcctcgggg
     5521 ttccaataag gcgcgccgag tccggtgaag gcgggcacca tcatcacacc gccggcatcg
     5581 ggcacgctct cggccagcgc ttgcacgtca gaactgctgg caattgcgcg caaaccgtcg
     5641 cgcagccaat gcaccaccgc accaccgaca aagacgctgc cttcaatggc aaattcgggc
     5701 tgctgggtgg tttgtgcagc gctggtgctg atcaggccgc tgttggaggt ctgaaatctg
     5761 gtgcccgtgt gcatcagcat gaagcagcca gtgccgtagg tgttcttggc catcccggcc
     5821 ttgaagcagg cctggccaaa cagggcgctt tgctggtcgc ctgccacacc gccgatagtt
     5881 acgccatggc cgagcaggtc ggtggccact tcaccgtaaa gcgcgctaga tggtttgacc
     5941 tgtggcaaca gatctgccgg gacgtcaagc gcctgcaaca agtcttcgtc ccactggttg
     6001 ctgtggacat tgaacagcat ggtgcgcgcg gcgttgctga catcggtggc atgcaccgca
     6061 cccctggtca gttgccagat cagccagcta tcaaccgtgc caaaggccag ctcaccgttt
     6121 tgcgcctgct gccgcgcgcc agcaacatgg tccagcagcc acttgagctt ggtgccggaa
     6181 aaataggcgt caaccagcaa gccggttttg gcccggatgg ccggggccag accgcgttcg
     6241 cgcagttcca ggcaggccgg ctctgcccgg cggtcctgcc agacgatggc gttgtgaatc
     6301 ggcaaaccgg ttttgcggtt ccagaccacg gtggtctcgc gctggttggt aatgcccacg
     6361 gcgcgcatgt cgcgggccgt cagaccggct ttggcgagtg ccgtgcgggc ggtggcgagc
     6421 tgggtgcgcc agatttccat cgggtcatgc tcgacccagc cgggctgcgg gtagatttgg
     6481 ctgagttcct gctgggctag tgcgacgata tggccattgg catcaaaaac aatgctgcgt
     6541 gaactggagg ttccctggtc aagggcgagt aagtaggtca tgtaatcacc tcggatcaac
     6601 gtgatggaaa ttaggtgctt ggccggcgcg ctctcagact atggccagtt cgcagtccac
     6661 ctgtgcatcg cgcagcaagg cggggaacgg atcaggcggt ggtgcgtcgg taaaaaggcg
     6721 gtcgatttgc gacagcttgc ccacttccac catcgccgga cggttgaatt tgctactgtc
     6781 tgcggccagc cagatttggc gggcatgggt caggatggtt tgggtcacct tgacttcacg
     6841 gtagtcaaag tcacgcaacg aaccatctgg ctcgatgcct gaaatgccaa tgatcgcaat
     6901 gtccaccttg aactggcgga tgaagtccac cgcagcctct ccgacgatgc cccggtcgcg
     6961 cccgcgcacc acgccgccca ccacgatgac ctcgcaattg gtgttggcgc tcagggtggt
     7021 cgcaacgttg aggttgttgg tgatgacgcg cagccccagg tggcgcacca gtgcgcgtgc
     7081 aatgcattcg gtggtggtgc cgatgttcag catcaatgag caatgattgg ggacggctgc
     7141 cgcaactgca cgggcgatgc gcaatttgcc ggcggtgttc aggttttcgc gttgctggtg
     7201 ggcaatgttc tcaaccgttg agccgggcgc acgtacacca ccatgaaaac gggtcagcaa
     7261 gccgttgtca gccagccgct gcacatcgcg tcgcacggtt tgcaatgtga cgccgagttg
     7321 gtcagccaga tgttccacgg tgacggagcc ctgggcttgc acgacggaaa gcagtgtcaa
     7381 ctgtctgggg ttggggttca tcgcggtttt caatgccgaa ttcagcaggc ctgactttaa
     7441 aacgaacagt taagaaaaca atagggtaaa taccgataaa aaaggaaacg aaacgaacaa
     7501 taattcactc acaaatttga cagcaaaggc aacgggatgc agttgactct tgagggcgtt
     7561 ggcaaaacag taggtgcaca gacatggttg catgacatga gtattgcgcc gcgcagcagt
     7621 gccgtcacgg tgttgctggg cgcaacccag gctggcaaga ccagcctgat gcgcatcatg
     7681 gctggtctgg atgtgccaac ttgcggacgg gtattggtcg atggtcagaa tgtggtgggt
     7741 gtgccggtgc gtcagcgcaa tgtcgccatg gtgtaccagc agttcatcaa ctacccgtcg
     7801 ttgaaggtgt gggacaacat cgcgtcgcca ctgacattgc gtggcgaaaa gaacattgac
     7861 caacgcgtgc ggcaactggc cgaaaagctg cacatcgaca tgttcctgga ccgctatccg
     7921 gccgagctct cgggtggcca gcagcagcgt gtggcgctgg cacgtgcact ggccaagggc
     7981 gctccgctga tgctgcttga tgaaccgctg gtcaacctgg actacaagct gcgcgaagaa
     8041 ttgcgtgatg agttgactga actgtttgct gcgggcagct cgaccgtgat ttacgccacg
     8101 accgaacccg gtgaggcgct gctgctgggt ggtcatacgg ctgttctgga tgcgggcgaa
     8161 ctgctgcagt acggtccgac cgctgaggta ttccacaaac ccaggtcctt gcgtgtggcg
     8221 cgggctttca gtgacccgcc catgaacctg atggtggccg atgcgactgc agcgggtgcc
     8281 gtgcagttgg tgggtggtcc gttgttgtcg atagtgctgc caacaactgc atcgcacaag
     8341 ttcacgcttg gcttgcgcgc cagcgccttg cggatacagg ctatggcagg tgatgtttct
     8401 ttgccgggca aagtcgagct ggccgagatt tccggctctg acacctttgt gcatgtagaa
     8461 acactggttg gggacctggt ggcgcagttg accggggtgc actattttga gctgggtgtg
     8521 caagttacca tttacttcag tccggaccag gtgtttgttt ttgacgacca gggtctgctg
     8581 gtgctgtcgc cggcgcgtgt tggaggtaac tgacatggcg cgcattgagc ttgacctggc
     8641 ccacgcctac aaacctgggc caaagcagga cagcgattac gcgttgctgc cactgcagat
     8701 gacgtttgag gatggcggtg cctacgcttt gctggggccg tccggctgcg gcaagaccac
     8761 cttgcttaac atcatgtccg gtttgctggc accctcacag ggtggggtgc gttttgacgg
     8821 tgtcgatgtc acccggtttt cgccgcaggc gcgcaacatt gcgcaggtgt tccagtttcc
     8881 ggtgatttac gacaccatga cggtggccga gaacctggct tttccgctgc gcaaccggca
     8941 agttccaaaa gatgtcatca acaagcgggt cggcgagatt gccgaagtgc tggagatgag
     9001 tggccagttg aatcagcgtg cggccaatct cgcggccgat gaaaaacaaa aaatttccct
     9061 tgggcgaggt ctggttcgcc ccgatgtctc ggcggtgctg tttgacgaac cgctgacggt
     9121 catcgacccg catctcaagt ggcaactgcg ccgcaagatc aagcagattc accaggaact
     9181 caagctgacc atgatctatg tgacgcatga ccaggttgag gcacttactt ttgcacagca
     9241 ggtggtggtg atgacgcgcg ggcgtgcggt gcaggtgggt tctgccgcgc aactgtttga
     9301 gcgcccgagt catacctttg tcgggcactt cattggttcg cccggcatga attttttggc
     9361 tggccaagtt gctcagcaag tgctgcatct ggcggatgtg gttttgccat tgcatgccgg
     9421 gttggcgctg ccgtctggtg acatcaagct cggaatccga ccggagtacg tcacgctggc
     9481 atcgccacat gcccagggtg ccttgcccat gacgttggca cacgtccagg atgttggcac
     9541 ccacatcatg ttgagcgcca gttttgcagg ccagaccgtc aaggcgcggc tgcccgccaa
     9601 tttggggcac ctggccaccg gtgacttaat ctggttgaag gtgttgggcg accaaacctg
     9661 cttctataaa aatgaggaga tcgtggcatg agcgtgacca ccaagccggt caatcagaaa
     9721 gcctggttgt tgattttgcc ggtgctgctt tgcgtggctt tctcggccat tctgcccttg
     9781 atgactgtgg tgaactattc ggtgcaggac atcatttcgc ccgagcgccg ggtgtttgtc
     9841 ggtaccgagt ggtttgccgc catcatgcga gacgaggaac ttcacggtgc cctggtgcgg
     9901 caactgacgt tttctctttc tgtgctggcg gtggaactgc cgctgggcat tttgctggct
     9961 ttgtcgatgc cagcgcaggg ctggaagtct tccgcagtgc tggtggttgt gtcgctctcg
    10021 ctgctgattc cctggaacgt ggtgggcacc atctggcaaa tcttcgggcg tgccgatatt
    10081 ggtttgatgg gcgcggcgtt gcaaggcctg ggcattgatt acagctatac cggcaatgct
    10141 gcccacgcct gggcaacggt gttgttgatg gatgtgtggc actggacacc tctggtcgca
    10201 ctgctgggct acgccgggtt gcgttccatt ccggatgcct attaccaggc tgccagcatt
    10261 gacggggcca gcaaatttgc tgtgttctgg tacgtgcagt tgcccaagat gcgaggggtg
    10321 ctgatgattg cggtgctgct gcgcttcatg gacagtttca tgatctacac cgagcccttt
    10381 gtgctgacgg gcggcgggcc tggcaactca accacatttt tgtcacagtt cttgacgcaa
    10441 aaggctgtgg ggcagtttga tctggggcct gcggctgcgt tttcctt
//
LOCUS       DAOSLL010000079        10444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6238_length_10444_cov_4.480797, whole genome shotgun sequence.
ACCESSION   DAOSLL010000079 DAOSLL010000000
VERSION     DAOSLL010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10444
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6238_length_10444_cov_4.480797"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /gene="rpmA"
                     /locus_tag="PLB25_05125"
     CDS             <1..252
                     /gene="rpmA"
                     /locus_tag="PLB25_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HOE40998.1"
                     /translation="KKGGGSTRNGRDSKPKMLGVKMYGGQLISAGSIIVRQRGTQFHP
                     GSNVGIGKDHTLFALVAGHVSFAVKGALNRHTVSVTAAA"
     gene            379..1452
                     /gene="obgE"
                     /locus_tag="PLB25_05130"
     CDS             379..1452
                     /gene="obgE"
                     /locus_tag="PLB25_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HOE40999.1"
                     /translation="MKFVDEAYIDVSAGDGGAGCVSFRHEKYKEFGGPNGGDGGRGGH
                     VFAVADENLNTLVDFRFSRRHDAQRGQHGMGSDMFGAAGGDITLKMPVGTIISDAETG
                     EVLFELLKPGEVITIAKGGDGGFGNLRFKSAINRAPRQKTPGWPGEKRNLKLELKVLA
                     DVGLLGMPNAGKSTLITAISNARPRIADYPFTTLHPNLGVVRVGPEQSFVVADLPGLI
                     EGASEGAGLGHLFLRHLQRTRLLLHVVDMAPFDDAVDTVLQAKAIVNELKKYDANLYH
                     KPRWLVLNKLDMVPKEERAALVKDFVSRLKFKGPVFEISALTHEGCEPLIKIIYQHIS
                     AQQKTEQVPEVIDPRFAVAAGPL"
     gene            1508..2644
                     /gene="proB"
                     /locus_tag="PLB25_05135"
     CDS             1508..2644
                     /gene="proB"
                     /locus_tag="PLB25_05135"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HOE41000.1"
                     /translation="MLKKFDESILRDARRVVVKVGSSLVTNEGRGLDATAIGEWCRQM
                     SALVREGREVIMVSSGAIAEGMKRLGWTTRPSQIHELQAAAAVGQMGLAQMYETKLRE
                     NGLGSAQVLLTHADLADRERYLNARSTLLTLLKLGVLPVINENDTVVNDEIKFGDNDT
                     LGALVANLVEADALVILTDQKGLYSADPRKDPTAEFVAVAQAGDPQLEAMAGGAGSSL
                     GRGGMITKILAAKRAAGSGASTVIAWGREPDALLRLSHGESIGTLLVAPTQKQKARKQ
                     WMVDHLQLRGSVTVDAGAVQKLRVGGSSLLPIGMTAVEGDFSRGEVIAIRAADGVEVA
                     RGLANYASAEARLLCRKPSGAFEKLLGYMAEPEMVHRDNLVLSR"
     gene            complement(2687..3151)
                     /locus_tag="PLB25_05140"
     CDS             complement(2687..3151)
                     /locus_tag="PLB25_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HOE41001.1"
                     /translation="MSLLWSLALAAGAQAQLIADDPDWRESDTPTPPTFSRDKLLPLE
                     MPPYVTLQFGIDPATLSISPDGIVRYVVVTSNSSGSVSAFYEGIRCSKGEVKVYARAG
                     GTGSWTLAAKPEWRALSSNQPSRHAVIFARQAACTGAAAASSTDDIVRAMKK"
     gene            complement(3180..3797)
                     /locus_tag="PLB25_05145"
     CDS             complement(3180..3797)
                     /locus_tag="PLB25_05145"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HOE41002.1"
                     /translation="MLDRDGFRPNVGIVLLNQKNQVFWGKRIRTHSWQFPQGGIDRGE
                     TPEQAMIRELHEEVGLQREHIRIVARTRDWLRYEVPDRFIRRDSRGFYKGQKQIWFLL
                     QLVGHDWDLNLRATNHPEFDAWRWNDYWVPLEAVVEFKREVYVTALTELSRYVPRNDY
                     RSRFLRQGIRSLESEQNCADRPDACIDLPPDATLEAVPMTGEEHV"
     gene            3888..5630
                     /locus_tag="PLB25_05150"
     CDS             3888..5630
                     /locus_tag="PLB25_05150"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HOE41003.1"
                     /translation="MKASQFFISTLKEAPADAEVVSHKLMMRAGMIKKLGAGIYTLMP
                     MGLRVVQKVEQIVRQEMNRAGAVELSMPVVQPAELWMETGRFEKMGPELLRIRDRHGN
                     DFVVQPTSEEVITDIARQEIRSYKQLPKNLYQIQTKFRDERRPRFGLMRGREFIMKDA
                     YSFDRDQASAKASYQVMARAYRRIFDRFGLTYRAVAADSGAIGGDLSEEFQVIAATGE
                     DAIVYCPGSDYAANMEKAEALAPTGPRAAATQALVKTPTPGKSTCADVAELLGVPLQT
                     TVKSLVLATDTLNDDGEVVKTQVWLLLLRGDHDMNEVKVGKLPGLQTFRFATVPEIED
                     HFGCKPGYLGPLNLQKPVKLVVDREVAIMSDWICGANLPDFHLTGMNWGRDLPEPDLV
                     ADLRNVVAGDASPDGKGVLVIERGIEVGHVFYLGTKYSAAMKATFLDHNGKPQFMEMG
                     CYGIGITRLPAAAIEQNHDERGIIWPDALAPFTVVLCPINADRSAEVKAAAESLYAQL
                     LAAGVDVMLDDRGERPGAMFADWELIGVPHRVNIGDRALKEGKVEYQHRRDTVATAVP
                     LADIAALLIAKLAA"
     gene            5733..6323
                     /locus_tag="PLB25_05155"
     CDS             5733..6323
                     /locus_tag="PLB25_05155"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOE41004.1"
                     /translation="MCIGLSALYFQEQAEAGAQLEEPLVDAVRTALSSAIANTAPPVP
                     EFADTESRLVYLRWLGAMGERLKKKKPDWDTRREFLQTVWYESKRAGLEVSLVLGLIQ
                     VESNFRKFAVSSAGARGYMQVMPFWTRVLGDGDSSKLFHMQTNLRFGCVILRHYLERE
                     RGDMFLALGRYNGSRGKPPYPNAVFGAQKVWALQGR"
     gene            complement(6357..6683)
                     /gene="grxD"
                     /locus_tag="PLB25_05160"
     CDS             complement(6357..6683)
                     /gene="grxD"
                     /locus_tag="PLB25_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HOE41005.1"
                     /translation="MSDTQQRIDDLVKQNDVLLFMKGTASFPQCGFSGRAVQILKACG
                     VDAKAFKTVNVLEDDEIRAGIKDYSNWPTIPQLFVKGEFIGGSDIMMEMYESGELQTM
                     LGTSAA"
     gene            complement(7065..7283)
                     /locus_tag="PLB25_05165"
     CDS             complement(7065..7283)
                     /locus_tag="PLB25_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010927830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HOE41006.1"
                     /translation="MTYPNALEERLTNLEIKASFAEDLIDKLDQILIAQQDQIDRLTR
                     EVLHLRQAALEQSTSAPANLRDNLPPHY"
     gene            complement(7341..8288)
                     /gene="prmC"
                     /locus_tag="PLB25_05170"
     CDS             complement(7341..8288)
                     /gene="prmC"
                     /locus_tag="PLB25_05170"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HOE41007.1"
                     /translation="MTSPLAPGSLAQTLSVLQARGIDRLDAQLLLLHLLGKPPEQRSW
                     LLAHDSDALPAACQLSLEALVQRRLAGEPLAYLTGNKAFYGLTLQVSRDVLVPRPDTE
                     TLVDWALQQLPGEPTQQRANAAPWPGGAAGLPGSVPWSATGLTDKVPLSVLDLGSGSG
                     AIALALKATRPDLRVWATDMSSAALAVAQANARHLQLEVRFCQGCWFAALPADAPLFD
                     CIACNPPYIAMHDPHLAALRFEPASALTSGADGLDDLRCIISDAPAYLKAGGWLLLEH
                     GFDQAEQTRSLLVQRGFVAVQSRRDLAGIERCSGGQKPQ"
     gene            complement(8373..8636)
                     /locus_tag="PLB25_05175"
     CDS             complement(8373..8636)
                     /locus_tag="PLB25_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41008.1"
                     /translation="MITSDVIQIAGRRADVGSGAGIWPDQGDNSMTRRETCELTRLRH
                     GLAGTKSLVFSSLLSASEFFACAAMASSAMSCGIPAGEARFAA"
     gene            complement(8633..10126)
                     /locus_tag="PLB25_05180"
     CDS             complement(8633..10126)
                     /locus_tag="PLB25_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HOE41009.1"
                     /translation="MKLLPRHAMPQEIELKLSLPGADPAGLAVHLARLPLLARRKPIR
                     QALYNIYFDTPDQLLRQQRAALRLRRVGSAAQPQWLQTLKTGASDDSALSRRGEWETA
                     VTGPALSRDALKGTAWAAMDTDGSLFDRLMPCFVTQFERTTWLVRRPDGSVVEVALDI
                     GQIEADGRQAPICELELELKAGQSAALFDIARVIALVMAVLPANQSKAQRGFLPAQLG
                     LDQPQRAQPPRLNPDLAGVELAQCVLLERFAQFTTNLNALGVSDDPEVVHQARVGWRR
                     FKSALRLFCKLPALASAPAWPELNALLSGLSELRNREVALTETLPPLEGAYCRGEAQR
                     AEAWQAALTTLTEAVALQRQVVRHALQDPAVGSNLLLMTQWLHDLAQTQHSGPEDKGE
                     LRQWAKRRVLRLQQRLHGAQKNVPTPQQQHQVRILAKRLRYGAEALRDLLPKRLARLC
                     GEQAAAIQTSIGATRDISQASALAAALELNPGIVEFLRGVELGLSLK"
BASE COUNT         2122 a         3087 c         3158 g         2077 t
ORIGIN      
        1 aaaaaaggcg gcggctctac gcgaaacggg cgcgattcca agcccaaaat gctcggtgtg
       61 aagatgtacg gcggtcaact gatcagcgcc ggctcaatca tcgtgcgcca gcgtggcacg
      121 caattccacc ccggctccaa tgtcggcatc ggcaaggacc acacgctgtt tgcgctggtc
      181 gcgggccatg tatcgtttgc cgtcaaaggt gcgctcaaca ggcacacggt gagcgtgacg
      241 gcagccgctt agtcagcgtt ctccccatga aaccctgcgc gtggcgcggg gttttttgtt
      301 tctgaagcct tgtgggacag accaattgcg ctgagcgcag caactatcgt tggtcggtcc
      361 tcaactgata atcaaagcat gaaattcgtc gacgaagcct acattgatgt ctctgccgga
      421 gacggcggag ccggctgcgt ctcttttcgg cacgaaaagt acaaggaatt tggtggtcca
      481 aacggcggcg acggtggccg cggtggccat gtttttgcgg tggcagacga aaacctcaac
      541 accctggtgg acttccgctt ttcgcgccgc catgacgcgc agcgcggcca gcacggcatg
      601 ggttccgaca tgtttggtgc cgctggcggc gatatcaccc tgaagatgcc ggtcggcacc
      661 atcatcagcg atgccgaaac cggcgaagtg ctgtttgaat tgctcaaacc cggcgaagtc
      721 atcacgattg ccaagggcgg cgacggtggc tttggcaatt tgcgcttcaa gagtgccatc
      781 aaccgggcac cgcgccagaa aacaccaggc tggccgggtg aaaaacgcaa cctcaaactt
      841 gaactcaagg tgttggccga tgttggcctg ctcggtatgc ccaacgctgg caagtcaacg
      901 ctgatcaccg ccatttccaa cgcacgtccg cgcattgccg actacccctt caccacgctg
      961 catcccaatc tgggtgtggt gcgggttggg cctgagcaaa gttttgtcgt ggcagatttg
     1021 ccaggtttga tcgaaggtgc gtccgagggt gccgggctgg ggcacctctt tttgcgccat
     1081 ttgcagcgca cccgtttgct gttgcatgtg gttgatatgg caccgtttga cgatgcggtt
     1141 gataccgtct tgcaagccaa ggcgattgtc aacgagctga aaaagtacga tgccaacctg
     1201 tatcacaagc cacgttggct ggtactcaac aagctcgaca tggtgcccaa agaggaacgt
     1261 gcggcgctgg tcaaagactt tgtcagtcgt ctcaaattca agggcccggt gtttgagatt
     1321 tctgcgctga cgcatgaggg ttgcgagcct ttgatcaaga tcatttacca gcacatcagc
     1381 gcccagcaaa aaaccgagca ggtacctgag gtgattgatc cccgctttgc ggtcgcagcc
     1441 gggcctcttt gaatgctact atgttaatag ctgcttgcgc agactggata agggctagag
     1501 cctgaaaatg cttaagaaat ttgacgaatc aatcctgcgt gatgcgcgcc gtgtggtggt
     1561 caaggtgggt tcgagcctgg tgaccaacga agggcgcggg ctcgatgcca cagccattgg
     1621 cgaatggtgt cgccagatgt cggcgctggt gcgcgagggc cgcgaagtca tcatggtgtc
     1681 aagcggtgcc atcgccgagg gcatgaagcg actcggctgg accacccgcc cgagccagat
     1741 ccacgagctg caggccgccg ccgcagtggg ccagatgggc ctggcgcaaa tgtatgaaac
     1801 caaactgcgc gaaaacggtt tgggcagcgc gcaggtgctg ctgacgcatg ctgacctggc
     1861 ggaccgtgaa cgctatctga acgcccgctc taccctactg acgctgctga aattgggtgt
     1921 gctgccggtg atcaatgaga acgacacggt tgtcaatgac gaaatcaagt ttggcgacaa
     1981 cgacacgctg ggtgcgttgg tggccaacct ggtcgaggcc gatgccctgg tgatcctgac
     2041 cgaccaaaaa gggctctaca gcgcagaccc gcgcaaagac ccgactgcgg agttcgttgc
     2101 ggtggcgcaa gccggtgacc ctcaactcga agccatggcc ggtggtgcag gatcaagcct
     2161 gggacggggc ggcatgatca ccaaaatatt ggcagccaag cgcgctgccg gttctggtgc
     2221 atcgaccgtg attgcctggg gccgcgagcc cgatgcactg ttgcgattga gccatggcga
     2281 gtcgattggc acgctgttgg tagcgcccac acagaagcag aaagcgcgca aacaatggat
     2341 ggtcgatcac ctgcaactgc gcggctcggt gacggtcgat gcgggtgcgg tacaaaagct
     2401 gcgcgttggc ggctccagcc tgctgccgat tggcatgacc gccgttgaag gtgatttctc
     2461 gcgcggtgag gtgatcgcca tccgcgcagc agatggtgtt gaggtggcgc gcggcctggc
     2521 caactacgcc agtgccgagg cacgcctgct atgccgcaag ccctctggtg ctttcgaaaa
     2581 actgctcggc tacatggcag agcccgaaat ggtgcaccga gacaacctgg tgctaagccg
     2641 ctaggcatct gccagaaaag cggtgccagg caagggttct gggcagctat ttcttcatcg
     2701 cccggacgat gtcatccgtt gaacttgccg cagccgctcc ggtgcaggcg gcctgacgtg
     2761 cgaagatgac agcatgtctg gacggctggt tgctgctgag ggcacgccac tcgggttttg
     2821 cggccagtgt ccaggaacca gtgccaccgg cacgggcata gaccttgacc tcacccttgg
     2881 aacagcgaat gccctcataa aaagcactga ctgatccgct gctgttactg gtgaccacca
     2941 cgtagcgcac gatgccatcc gggctaatgc tcagcgtggc cgggtcaatg ccgaattgca
     3001 aggtgacata cggcggcatc tcaaggggta gcagtttgtc ccggctgaaa gtcggcggtg
     3061 tcggcgtatc tgactcccgc cagtctggat catcggcgat gagctgcgcc tgagcacctg
     3121 cggccaaggc caggctccaa agcagactca agaatcggta attttttcgg aaaaaatgct
     3181 caaacatgct cttcgccggt cattggaacg gcctccaatg tcgcgtccgg cggcagatca
     3241 atgcaggcat cgggccggtc tgcacaattt tgctctgact ccagggagcg aatgccttga
     3301 cgtaaaaaac ggctacggta gtcattgcgc ggcacgtagc gcgacagttc agtcaaggcc
     3361 gtgacataga cttcgcgctt gaactccacc accgcttcca gtggcaccca gtagtcgttc
     3421 cagcgccagg catcgaattc gggatgattg gtggcgcgca ggttcaaatc ccagtcgtgc
     3481 ccgaccaact gcagcaaaaa ccagatttgc ttttgtccct tgtaaaaacc gcgcgaatcg
     3541 cggcggatga aacggtctgg cacctcatag cgcaaccagt cacgggtgcg ggccacaatg
     3601 cgaatgtgtt cacgttgcag cccaacttct tcgtgcaatt cccgaatcat ggcctgctcg
     3661 ggggtctcac cccggtcaat gccaccctgc ggaaactgcc aggaatgcgt ccgaatacgc
     3721 ttaccccaaa acacctgatt tttctggttg agcaggacga tgccgacgtt cggcctaaag
     3781 ccgtctcggt caagcataat caaaccccaa atttgtaaat tgtgtccatt atgcacagcg
     3841 tttgcctggc atcaagagcg acaactgact taattccgga tacccccatg aaagcctccc
     3901 aattctttat ttctaccctc aaagaggctc cggccgatgc cgaagtggtc agccacaagc
     3961 tgatgatgcg tgccggcatg atcaaaaagc ttggtgcggg catttacacc ctgatgccga
     4021 tgggcctgcg cgtggtgcaa aaagtcgagc aaatcgtgcg ccaggaaatg aaccgcgcag
     4081 gtgccgtgga attgtcgatg ccggtggttc agcctgctga actgtggatg gaaaccggcc
     4141 ggttcgagaa aatggggccg gagttgctgc gcatccgtga ccgccacggc aacgacttcg
     4201 tggttcaacc caccagcgaa gaagtgatca ccgacatcgc ccgccaggaa attcgcagtt
     4261 acaagcagtt gccaaagaat ttgtaccaga ttcagaccaa gttccgtgac gagcgccgtc
     4321 cgcgctttgg cctgatgcgg gggcgcgagt tcatcatgaa agatgcctac agcttcgacc
     4381 gtgatcaggc cagcgccaag gccagctacc aggtcatggc ccgggcttac cgccgcattt
     4441 ttgaccgttt tggcctgact taccgcgcgg tggcagccga cagcggtgcc attggcggcg
     4501 atttgagcga agagttccag gtgattgccg ccaccggcga agatgccatc gtctattgcc
     4561 cgggcagcga ttacgccgcc aacatggaaa aagccgaggc gctggccccg actggcccgc
     4621 gcgctgcggc cacgcaagca ttggtcaaga ccccgacacc tggcaaaagc acatgtgccg
     4681 atgtggcaga actgctgggc gtgccactgc aaaccaccgt caaatcgctg gttctggcga
     4741 ctgacaccct caatgacgat ggcgaagtcg tcaaaacgca ggtgtggctg ttgctgctgc
     4801 gtggtgacca tgacatgaac gaagtcaagg ttggcaaact gcccggcctg cagaccttcc
     4861 gttttgccac ggtgcctgag attgaagacc actttggctg caagcccggc tatcttggcc
     4921 cgttgaatct gcaaaagccg gtgaaactgg tggtggaccg tgaagtggcg atcatgagcg
     4981 actggatttg tggcgccaac ttgcctgact ttcacctaac cggcatgaac tggggccgtg
     5041 acctgcccga gccagacctg gtggcagacc tgcgcaacgt ggtggccggt gatgcgtcgc
     5101 ccgatggcaa gggcgtgctg gtgatcgagc gcggcattga agttggccat gtgttttacc
     5161 ttggcaccaa atacagtgca gccatgaaag ccacctttct ggaccacaac ggcaagcccc
     5221 agttcatgga gatgggttgc tacggcatcg gcatcacccg cctgcccgct gctgccatcg
     5281 aacaaaacca cgacgagcgc ggcatcatct ggccagatgc gctggccccg ttcaccgtgg
     5341 tgctgtgccc gatcaatgca gaccggtctg ccgaggtcaa ggccgcagcc gagtcgctgt
     5401 atgcccaact gctggccgcc ggtgtcgatg tgatgctgga cgaccgtggc gagcggcctg
     5461 gtgccatgtt tgccgattgg gaactgattg gcgtgccaca ccgcgtcaac attggcgacc
     5521 gcgccctgaa agaaggcaag gtggaatacc agcaccggcg cgacaccgtt gccactgcgg
     5581 tgccattggc agacattgct gccctgttga ttgccaagtt agcagcttga gtggtttgca
     5641 cacctgttga cgctaaattt gaccgggttg ccgactttgc tggcagtctc acacgccgcg
     5701 ccctgattat tggttctttt ggcctcaagc ccttgtgcat tggcctgagc gcgctctatt
     5761 ttcaagagca agccgaggcc ggtgcacagc ttgaagagcc gctggttgac gcggtccgca
     5821 cggcccttag ctcagccatt gccaataccg ccccaccggt accggagttt gccgataccg
     5881 agtcgcgcct ggtgtacctg cgctggcttg gtgccatggg cgagcgtctg aagaaaaaaa
     5941 agccggactg ggacacccgc cgggaattcc tgcaaaccgt ctggtacgaa agcaaacgag
     6001 ctggcctgga ggtgtcgctg gtgttgggcc tgattcaggt agagagcaat tttcggaaat
     6061 ttgccgtcag cagcgccggt gcccgtggtt acatgcaggt catgccattt tggacccgcg
     6121 tgctgggcga tggggattcg agcaagctgt ttcacatgca gaccaacctg cgttttggct
     6181 gtgtcatcct gcgccattat ctggaacgcg agcgcggcga catgtttctg gcgctgggcc
     6241 gctacaacgg ttccagggga aaaccgccgt acccgaatgc ggtgttcggg gcccagaaag
     6301 tctgggcgct gcagggccga taggctctca aagcgtcacc ggccagcaaa ccagcttcag
     6361 gcggcgctgg tgcccagcat ggtttgcagt tcgccgctct cgtacatctc catcatgatg
     6421 tcggagccgc caataaattc acctttgaca aagagttgcg gaatcgtcgg ccagttgctg
     6481 tagtccttga tgccagcccg gatttcgtcg tcctccagca cgttgacggt cttgaatgcc
     6541 ttggcatcga caccacaagc cttgagaatt tgtaccgcgc ggcccgaaaa accgcactgg
     6601 ggaaaactgg ctgtgccctt catgaacagc aatacgtcgt tttgcttgac cagatcatcg
     6661 atgcgttgtt gtgtgtcact catgctcaaa aattcctttg tgaaaaaaca gcccgaatta
     6721 tttcactttc gccaatagcc agggctgccg ggcaaatttg ttcctgtagc agttctatca
     6781 gtgtcgggaa ggctggcatc caggggtact tggagcctgc cagaccttac cggcttcgat
     6841 ggtgttgcaa attgctacta tgtttatagc ttgtcacgct tgtcccataa gggctagcgg
     6901 caaatttgca tctattgtgg gcaagcctcg gcgcgctaaa ggcatttccg gaggaacacc
     6961 tggccgggcc aattcggccg caatgccgtg ggctgctggt ttctggctac ggtcgccttc
     7021 atcaatcccc cgaactgcga tttgatgcat ggagttggca tgtttcagta atgcggcggc
     7081 aagttgtcac gcaggttagc tggcgcgctg gtgctttgtt ccaacgcggc ttgacgcagg
     7141 tgcagcactt cacgggtcag gcggtcgatc tggtcttgct gggcaatgag aatctggtca
     7201 agcttgtcga tcaggtcttc ggcaaagctg gccttgatct ccagattggt caggcgctct
     7261 tcgagtgcgt tcggataggt catgggatac aaccggtggg tggtgaaaaa cgggtcaggc
     7321 gtggcggcgg tcattgaggg tcattgaggt ttctggccac cgctgcagcg ctcgatgcca
     7381 gccaggtcac ggcgggattg aacggcgaca aaaccccgct gaaccagcaa gctgcgggtc
     7441 tgttcggcct ggtcaaagcc atgttcgagc agcagccagc ctccggcttt cagataggcc
     7501 ggggcatcgc tgatgatgca acgcaggtcg tcaaggccgt cagcgccact ggtcagggct
     7561 gacgccggct cgaagcgcag cgccgccagg tgcgggtcgt gcatggcaat atagggcggg
     7621 ttgcaggcaa tacagtcaaa cagcggggca tccgcaggca acgcagcaaa ccagcagccc
     7681 tggcagaacc gcacctcaag ctgcaggtgc cgggcgttgg cctgtgccac cgccagcgcg
     7741 gctgaactca tgtcggtggc ccacacccgc agatcagggc gagtggcttt gagcgccagg
     7801 gcgatggcac cgctgccgct gcccaggtcc agcacagaca gtggcacctt gtcggttaaa
     7861 ccggtcgctg accaaggcac gctgccgggc agcccagccg caccgccggg ccatggcgca
     7921 gcgtttgccc gctgctgggt gggttcgcct ggcaattgct gaagcgccca gtccaccagg
     7981 gtctcggtgt cgggccttgg caccagcacg tcgcggctga cctgcaaggt caacccgtaa
     8041 aacgccttgt taccggtcag gtaagccaat ggttcaccgg ccagacggcg ctgcaccagc
     8101 gcctctagcg acaactggca cgctgctggc agtgcatcgc tgtcatgcgc gagcaaccag
     8161 ctccgctgct cgggcggctt gcccagcaga tgcagcagca gcaactgcgc gtccagccgg
     8221 tcaatgccgc gcgcctgcag caccgacagc gtctgcgcca gactgcccgg cgccaaagga
     8281 ctggtcatcc attacctcgg cacagcacct gaccggcgaa cccggcggga gttgacaacg
     8341 gcgaactttg acaagcttgc atggcgttct gatcaggcag caaaccgggc ttcaccggcg
     8401 gggattccac aactcatggc agaacttgcc attgccgcgc aggcgaagaa ttcggacgcg
     8461 ctcagcaagc tgctgaaaac cagggatttc gtgcctgcca ggccatgacg caatcgcgtc
     8521 agttcgcaag tttcgcgacg ggtcatcgag ttgtcgccct ggtctggcca aatgccagcg
     8581 cctgacccaa catcagcccg cctgccagcg atctggatca catcactggt gatcatttca
     8641 ggctcaatcc caactcaacg ccacgcaaaa attcgacgat tccagggttc agttcaagcg
     8701 cggcggccag cgcgctggct tgcgagatat cgcgcgtggc accaatgctg gtttgaatgg
     8761 ccgccgcctg ttcaccgcac aacctggcaa gccgcttggg caacaggtca cgcaacgcct
     8821 cggccccata acgcaagcgc ttggcaagga tgcgaacctg gtgctgctgc tgcggggttg
     8881 ggacattttt ctgcgcgcca tgcaggcgct gctgcagacg caaaacccgc cgcttggccc
     8941 attggcgcaa ctcccccttg tcttcaggtc cgctgtgctg cgtctgcgcc aagtcgtgca
     9001 gccattgcgt catcaacagc aaatttgacc cgaccgccgg gtcctgcagc gcatggcgaa
     9061 ccacctggcg ctgcaaggca accgcctcgg tcagcgtggt cagcgcggcc tgccatgcct
     9121 cggcacgctg ggcctcgccc cggcaatagg cgccttccag gggcggcagc gtctcggtca
     9181 gggccacttc ccggttgcgc aattcgctca agccagacag cagcgcatta agttcgggcc
     9241 aggccggtgc gctggcaagg gcaggcagct tgcaaaaaag acgcagcgct gacttgaagc
     9301 gccgccagcc cacccgcgcc tgatgcacca cctcggggtc atccgatacg ccaagcgcat
     9361 tcaggttggt ggtgaactgc gcgaaccttt ccagcagcac gcattgcgcc agttcgaccc
     9421 ccgccaggtc cggattgagc cgtggcggct gcgcccgttg cggctggtcc agcccaagct
     9481 gcgccggcaa aaagccgcgc tgcgccttgc tctggttggc tggcaaaacc gccatgacca
     9541 gcgcgatgac gcgggcaatg tcaaacaggg ccgcactctg acctgccttg agttcgagtt
     9601 ccagctcgca aatcggtgcc tgccgaccat cggcctcgat ctgaccgatg tccagcgcca
     9661 cttccaccac actgccatcg ggcctgcgca ccagccaggt ggtgcgctca aactgggtca
     9721 caaaacacgg catcaggcgg tcaaacaggc tgccatcagt atccatggct gcccaggcgg
     9781 tacctttgag cgcatcccgg ctcaaagcgg gaccggtcac tgcggtctcc cattcgccgc
     9841 gccgactcag ggccgaatcg tcgctggcac cggttttcag cgtttgcagc cactgtggct
     9901 gcgccgcgct gccaacccgg cgcaagcgca aggcggcccg ctgctggcgc aacaactggt
     9961 ctggcgtgtc aaagtaaatg ttgtacagcg cctgccgtat tggcttgcgc cgggccagca
    10021 agggcaagcg ggccaggtgc acggccaggc ctgccggatc ggcgccgggc aaggacagtt
    10081 tgagttcaat ctcttgcggc atggcgtgac gggggagtaa tttcatcggg tattgacctt
    10141 aacgcattgt caggcacgat tgccagcctt gcactggcta ctgacatgct atttattata
    10201 tagctgtcag cgctttattc gtaagggcta aaggcacttt tggcatcaaa caatgcatga
    10261 gtgcgatgac tcacaccaga ccggcacctt gttgccggtg cgccgtggtt cggggttcgg
    10321 gcatcggtct ggctgactgt cttgggccga catgcagcgc ttgcccgatc agtcatcgca
    10381 atcctctctg gtcccgcgct ggcggggaac ctgaggcgct tgcaatctgt tgaaaaaaaa
    10441 catt
//
LOCUS       DAOSLL010000080        10436 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6250_length_10436_cov_4.596667, whole genome shotgun sequence.
ACCESSION   DAOSLL010000080 DAOSLL010000000
VERSION     DAOSLL010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10436
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6250_length_10436_cov_4.596667"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLB25_05185"
     CDS             <1..308
                     /locus_tag="PLB25_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009455161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HOE41010.1"
                     /translation="MPDKVKYFLWLPQSATTSWYSATWLYPETTLADPKFKERWQMER
                     DDLYPVMIEDYTAWRRYQVGGESRFAARGRLSSYERVIGRYQDWLVNKYRKASEAGA"
     gene            321..1280
                     /locus_tag="PLB25_05190"
     CDS             321..1280
                     /locus_tag="PLB25_05190"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDR/VanB family oxidoreductase"
                     /protein_id="HOE41011.1"
                     /translation="MQTQNAIEVKVVAKAITAVSTAVIDLETLDGSDLPAWKAGSHIE
                     LSWSVDGQDFVRQYSLCGERFSGPRWRIAVHREKNSRGGSAYLCDQVSVGTRVKVRCP
                     RNNFPFVPDQRVTFLAAGIGITPLLPMAFEAEAQGCDWRLVYLVCEREDVSLRETLDK
                     LPPHRVSYYFSAEVGRLNLGEWTRGLSDGDSVFVCGPLRLLDDMVALHEVNPIWKLHL
                     ERFENPNKDVGDAVAFDLVLARSGKSYRIQPNESILDVLRRDGMNLEWSCCDGVCGTC
                     EQVVVDGVPDHRDAVLTPEERKSNRHMMICVSRAATPSLTLDL"
     gene            1291..2151
                     /locus_tag="PLB25_05195"
     CDS             1291..2151
                     /locus_tag="PLB25_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003803672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE41012.1"
                     /translation="MSIKKVVVTGGSGRVGTYVLRELLPHFDVTNADLVEGRVDTQYV
                     RADVMDLDSLRRAFVGADAVVHLAAIDFDWKAAPEKYIDVNVRGTWHVLQAASEAGVK
                     KVVLCSSVSACGLSEMRSDWHPQYLQVDEQHECRPVQAYSVSKQLMEQMGLSFVHGTA
                     MDVICLRPLAVIMQETFAEYVKFVEEPNRNWLFYYVTATDVARGFRAALDVKGVRYGT
                     FFLSAADTSRPEPTVEWYRHLFGDVPPLKNPEWFKAQPRASVFSSAKAKDMLGWEPAS
                     NYLDLRALWL"
     gene            2173..2802
                     /locus_tag="PLB25_05200"
     CDS             2173..2802
                     /locus_tag="PLB25_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dehydratase"
                     /protein_id="HOE41013.1"
                     /translation="MITTVKPGAVDWTGDNPFIYLKTDPNGDWSTLALYFRIALSPYG
                     VGQAMMVLEAPYQPARLEARRLVLTDNPEMTRYLLNGFVKHFGLFRPCTDLLDDIRIV
                     GDSHFSCHDQGLGSHVQRAVSTQAGINIEMHWENLQLPFMAVVPADKTQTGQHEMFTV
                     FQPAGKGWVSFNGEILPGTTVEREFFGGRAQSAALAFGETWVQSEHETV"
     gene            2810..4009
                     /locus_tag="PLB25_05205"
     CDS             2810..4009
                     /locus_tag="PLB25_05205"
                     /inference="COORDINATES: protein motif:HMM:NF018275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HOE41014.1"
                     /translation="MYEHHPRPHPVDLCDESAVKKVLAVRPHCTGVHRLDDVIRNTNR
                     RIFHAGPPYRSLAEIPAPVLNSAAQAVVYEGWCHTVTEAIEAIREGQILMASAQANNL
                     MVPLAGVLSASMAVLEISDPKLELASVFVALNEGQVHATRLGRLDDGLLPHLHWLNGL
                     FAGWVKQCLVSPLDLLPLLVQARLAGDDCHARTVVGSEMIAAELLKRGSEPGNEIRNF
                     LKASPAFALNFWMGATALAASVAEGLSGSSLITRAGGNGVEFGIQLAAHPGRWICCPA
                     PIPKGMVEAVHVGRHAVGALGDSAVVDFSGLGGQSLHKAPLVAQGVELYLPSDVARRP
                     SQVLASAGRDVSHGLGATSAFRCVEAGAGPLILIGMIDAQGQSGRIGSGVIDVSVAIF
                     EQALKTL"
     gene            4020..5336
                     /locus_tag="PLB25_05210"
     CDS             4020..5336
                     /locus_tag="PLB25_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HOE41015.1"
                     /translation="MSSLNQLSLTGMLNAIEQGDCLPSDIWRSCLHQIDRYDRDVHAW
                     QQLYCLADESQAIHLENTRFPLQGLPIGIKDTIDVLGMRAERGSRVWQGREAPADASC
                     ISALKHAGGRVIGKTVTTEFAYFTPGATANPHNLAHTPGGSSSGSAAAVAAGMVPVAL
                     GSQTAASLIRPAAYCGVVGYVASVGSVSLRGVMPLAHTLDALGLVTRDVADVHYVQAL
                     LTRAPVKQGRVRRKPEAVLAVDGRAFGAVDAEMLALHQRTLDGLASQGVRIYTVTDEL
                     GAEGGQKLAQWHHELMAYEAAQTLSFEYHEHADGLSRALQDLIEEGLGIDGERYEELK
                     SLRDMAMAKFARAMDRYDAVLAPAAPGAAPRGLEATGRPDQSRAWQLLGVPQITLPAG
                     VDDASMPLGMQLIGRQRGDVRLLDIALWFQDELGWKGEIPPAFRRQ"
     gene            5397..6581
                     /locus_tag="PLB25_05215"
     CDS             5397..6581
                     /locus_tag="PLB25_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006406801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE41016.1"
                     /translation="MDTVVITPRSGLDMHVFGSTRQVSGEAIRTFALGKPYVTFEHVQ
                     KTYDGNSLVVRDLNLQVAKHEFLTMLGPSGSGKTTCLMMLAGFETATSGEILLDGRPV
                     NRIAPEHRDIGMVFQSYSLFPHMSVSQNLAFPLRIRKVPRHDIEKRVKRALEMVNLGA
                     FGDRMPAQLSGGQQQRVAVARALVFEPKLVLMDEPLGALDKQLREQMQYEIKRLHKEL
                     GLTVVYVTHDQDEALTMSDRIAVFKDGVIQQLASPTDLYERPTNAFVASFIGENNEWS
                     VTVTARKADLCDVRTQGGVTLVALNVADLKQGQQAVLSVRPERLVFDADFRESANACN
                     VIAAQVMDVSYHGDHLRVQCALNGGDELIAKVPNGGTAMSLEKGQKVAVHWRVEDGRV
                     LS"
     gene            6668..7738
                     /locus_tag="PLB25_05220"
     CDS             6668..7738
                     /locus_tag="PLB25_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013689142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE41017.1"
                     /translation="MKIRKKALALAAIVSLTLVAHGVNAADLTFVSWGGTYGKSQAEA
                     LVKPYSAKTGHRILVEDYSGGLAQIRAQVKSNKVTWDVVDLELQDAIRACDEGLLAPI
                     QARDLQGVPGVASVVDDFLPNSLTPCSVGTVMWANVITFNLNKFVGSEPSTVGDFFDL
                     KKFPGKRGMKKAAKANLEWALMADGVAPADVYKVLGTPAGLDRAFRKLNTIKSSIVWW
                     EAGAQPPQLLADGAVTMTSAYSGRIQAAIDVDRKPFKIIWQGQIPEYETLAILKGSKN
                     EATARDFIKFASSPAVLAEQTKYIAYGPLRKSALSLIESSVVSKLPSAAANIAVGVPS
                     NPEWWANNGDEINQRFSVWLAN"
     gene            7820..9052
                     /locus_tag="PLB25_05225"
     CDS             7820..9052
                     /locus_tag="PLB25_05225"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006406802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE41018.1"
                     /translation="MDISINNSVELKRQLRSTQRAFRLRTFMLVMPLLVFLLLSFLAP
                     IGVVLYKSINDDVMTGIMPQSAAALREWDVRATSLPPQGTRDLVARELQAAAKSREIS
                     RVATRANFERPGMRSLLMSTSRKLMALEGTPDYEALIAADGRWGETDPWVGLKYISSD
                     FTYHYYLNALDLRQADDGSITSQPDDLKLYSDVSWRTFSISFQVAGLCLLLGFPLAYF
                     LANLPSRTSNMLMVFVLLPFWTSLLVRTMAWVVILQPEGVVNSALMSMDVLSDPLPLI
                     FNRFGVLVAMVHILMPYMVLTLYSVMKAIPMGYVRAARSLGAGPVMAFFNVYLPQTFP
                     GVFAGTLLVFILALGFYITPAIVGGAQDQMLSFFIADHMNNSLNWGLASALSGLLLVG
                     VFILYGLYARLTMRKGEK"
     gene            9052..9882
                     /locus_tag="PLB25_05230"
     CDS             9052..9882
                     /locus_tag="PLB25_05230"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE41019.1"
                     /translation="MMFPAYVSWKGKTCHLAYRVFCGLLLAFLALPVLVNIPLSFNSE
                     PYFSYPLSGFSLKWYQELLNEPQWLLALKNTLIVGVASTSIATVLGVTAALGLAERDL
                     PMRHIVKAIFISPMVVPVVTAGVGMYFLYSKFDLTGNLLGLIIAHAALGTPFVVMTVE
                     ATLVGYNASLTRAARSLGASPLYAFFKVKLPLIAPGVISGAVFAFGTSFDEVVVVLFL
                     GGVEQRTVPRQMWSGIREQLSPTILAAATVLTVLSVLLLVVLEILRRRGEQMRGAVSH
                     "
     gene            9898..10167
                     /locus_tag="PLB25_05235"
     CDS             9898..10167
                     /locus_tag="PLB25_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41020.1"
                     /translation="MRRSTGQQPTRNILGIGAMVLGVALFAWVDTILKLLVEYYPPLQ
                     VAGLRGLVALPILSVWIHWRGAWGSLFRIRVRLQGSSQKTENKAR"
     gene            complement(10164..>10436)
                     /locus_tag="PLB25_05240"
     CDS             complement(10164..>10436)
                     /locus_tag="PLB25_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HOE41021.1"
                     /translation="EIRDRSFEQQRYRASGLNLVTAAIVLWNTAYLERATNALNGHGK
                     PVDETLFQYLSPLGWEHINLTGDYLWRSSTKVGAGKFRPLRPLPPA"
BASE COUNT         2014 a         2660 c         3235 g         2527 t
ORIGIN      
        1 cgatgcctga caaggttaaa tatttccttt ggctgccgca gtcagcaaca acgtcctggt
       61 atagcgcaac gtggctctat ccagagacca cgctcgctga ccccaagttc aaagagcgtt
      121 ggcagatgga gcgtgatgat ctgtatcccg tgatgatcga ggactacacc gcatggcgcc
      181 gttaccaagt cgggggcgaa tcgcgatttg ctgcccgtgg tcgtctgagc tcgtatgaac
      241 gggtgattgg ccgctaccag gactggttgg tgaacaagta ccgcaaggcg agcgaagcgg
      301 gtgcttgaac ggagtcttcg atgcaaactc aaaatgcaat tgaagtaaag gtcgtcgcca
      361 aggcgatcac cgcggtatcc accgctgtca tcgacttgga gacgcttgac gggagtgacc
      421 ttccagcgtg gaaggctggc tctcacattg aactcagttg gtccgtggat ggccaagact
      481 tcgttcgaca gtattcgttg tgcggtgagc gcttctcagg gccgcgctgg cgcatagcag
      541 tacatcggga aaaaaacagt cgtggaggat cggcatattt gtgtgatcag gtgagtgtgg
      601 gtacgcgtgt caaggtccgt tgtcctcgca ataactttcc cttcgtgccg gaccagcgtg
      661 tcacttttct ggctgccgga atcgggatta cgccgctgtt gccaatggcc ttcgaggctg
      721 aggcgcaggg gtgtgactgg cgtctggtct atttagtctg cgaacgtgaa gacgtcagct
      781 tgcgtgaaac cctggacaaa ctaccgccgc accgggtgtc gtactacttt tcggccgagg
      841 tgggtcgttt gaatttgggt gagtggacac gtggcttgtc tgatggggac tcggtcttcg
      901 tgtgtggtcc gctgcgtttg ctggacgaca tggtggccct tcatgaggta aaccctattt
      961 ggaagctgca tcttgagcgg tttgaaaacc ccaacaagga tgtgggtgac gctgttgcct
     1021 ttgatctcgt tctggctcgt tctggaaaaa gttatcgaat tcagcccaat gagtcgattt
     1081 tggacgtcct gcgtcgcgat gggatgaact tggaatggtc atgttgtgac ggtgtgtgcg
     1141 gcacgtgcga gcaagttgtg gtggatgggg ttcctgatca ccgtgatgcg gtcttgacgc
     1201 cggaagagcg gaaatccaac cgtcacatga tgatttgtgt atcccgggct gctacgccat
     1261 ctctaacgct cgatctttga aagactatcc gtgagtatca aaaaagttgt tgtaaccggt
     1321 ggctctggtc gcgtaggcac ttacgtgttg cgtgaattgt tgccccactt tgatgtgacc
     1381 aatgctgacc tggtcgaagg tcgagtcgat acccagtatg tgcgggcgga cgtgatggac
     1441 ctcgattcgt tgcgccgggc attcgtgggg gctgatgccg tggtgcatct cgccgccatc
     1501 gattttgact ggaaggccgc gccggaaaaa tacattgatg tcaacgtgcg agggacatgg
     1561 cacgtgctgc aggccgcgtc agaagccgga gtcaaaaagg tggtgttgtg ttcgagtgtg
     1621 tccgcttgcg gtttgtccga gatgcgctcc gattggcatc cgcagtatct gcaagtcgat
     1681 gagcaacacg aatgtcgacc tgtccaagcc tacagcgtga gcaagcagct aatggagcaa
     1741 atgggtttga gtttcgtgca tggcacggcc atggacgtga tctgcttgcg gccgctggcg
     1801 gtgatcatgc aagagacatt tgccgagtac gtcaagttcg tggaagagcc caaccggaat
     1861 tggctttttt actacgttac ggcgactgat gtcgctcgag gcttccgcgc ggcgttggat
     1921 gtcaaaggtg tgcgctacgg caccttcttc ctgagtgccg cagacactag tcgtcctgaa
     1981 cccactgtcg agtggtaccg gcatttgttc ggtgatgtgc ctccgctgaa aaatccggaa
     2041 tggttcaagg ctcagccgcg tgcgtccgtt ttttcgtccg ccaaggcgaa ggacatgctc
     2101 ggatgggagc ccgcgtcgaa ctatcttgac ttgcgcgcac tctggctttg aaccaacgag
     2161 gaaacaaacg cgatgattac tacagtaaaa cccggtgccg tcgactggac cggagacaac
     2221 ccttttattt atctcaagac cgacccgaat ggcgactggt caaccttagc actttacttc
     2281 cggattgcgc tgtcgcccta tggcgtgggg caagccatga tggtgcttga agcgccttat
     2341 caacccgcac gcttggaagc caggcggttg gtgttgaccg acaatcctga aatgacgcgg
     2401 tatttgctga atgggttcgt caaacacttc ggacttttcc ggccgtgtac cgacctgttg
     2461 gatgatattc ggattgtggg tgactcacat ttctcgtgtc atgaccaggg tcttggctca
     2521 cacgtgcagc gagcggttag cacgcaagcg ggcatcaaca tcgagatgca ttgggagaac
     2581 ttgcagctgc cgtttatggc ggtggtcccc gccgataaga cccagacggg tcaacacgaa
     2641 atgttcacgg tgtttcagcc cgctggcaaa gggtgggtga gctttaacgg tgaaattctt
     2701 cccgggacaa cggtcgagcg ggaattcttt ggtgggcgcg ctcagtcggc tgcgctggcg
     2761 ttcggtgaaa cctgggtgca gtccgaacat gaaaccgtct gagcccatga tgtacgaaca
     2821 ccatccccga cctcatccgg ttgatttgtg cgatgaatca gcagtcaaga aagtgttagc
     2881 cgtaaggcct cattgcaccg gcgttcatcg gctggatgac gtgatccgga acactaatcg
     2941 acgaatattt catgcgggcc cgccctatcg gtctctggcg gagatcccgg ctccggttct
     3001 aaattctgct gctcaagcgg tggtgtatga gggctggtgc cacactgtta ctgaggcaat
     3061 cgaagcgatc cgcgaggggc agattctcat ggcgtccgcg caagcaaata acctgatggt
     3121 gccactcgcc ggtgtgctga gtgcttccat ggcggtactg gagatttcag accctaaact
     3181 cgaattggcc tcggtgtttg tcgcgttgaa cgaaggtcag gtacacgcca cccgattggg
     3241 gcgactggac gatggcttgc tgccccacct gcattggctc aatggtttgt ttgcagggtg
     3301 ggtgaagcag tgcctggtgt caccgctgga cctgctgccg ttgctggtcc aggcccgctt
     3361 ggcgggcgat gattgccacg ccaggacggt ggtagggtct gagatgatag cggctgaact
     3421 gctaaagcgc ggctcggagc ctggcaacga gattcgaaat tttctgaagg cgtctccggc
     3481 attcgcgctc aatttctgga tgggggctac cgccttggcg gcgagtgtcg cggaaggcct
     3541 gtcaggctca tcgttgatca cgcgggctgg tggcaacggc gtggaattcg gtattcaact
     3601 cgcagcgcat cccggacgct ggatttgctg tcctgcccct attccgaagg gtatggtgga
     3661 ggcggtccat gttggacggc atgcagtggg ggcattgggc gacagtgctg tagttgattt
     3721 ttctgggctg ggggggcagt ctctgcacaa ggcgccgctg gtggctcaag gggttgagct
     3781 gtacttgccc agcgatgtcg cacgtcgccc atcgcaggta ttggcctcgg cgggacgtga
     3841 tgtgagccat gggttgggcg ctacctcggc gttccgttgc gttgaggctg gcgcgggtcc
     3901 tctgatcctg atcggcatga tcgatgcgca gggacagtcg gggcgaattg gcagtggcgt
     3961 gatcgatgtg tctgttgcga tctttgagca agcgctcaag acgctgtgag ggacagatta
     4021 tgtcatcact aaatcagctg agtttgacgg gcatgttgaa tgccatcgaa cagggcgact
     4081 gtctgccgag cgatatctgg cgcagttgcc tgcaccagat cgatcggtac gatcgggacg
     4141 tccatgcctg gcaacaactc tattgcctcg cggatgagtc tcaggccatc cacctcgaga
     4201 atacgcgctt tccgctacag ggtttgccca tcggtatcaa ggacactatt gatgttctag
     4261 gtatgcgggc cgagcggggt tccagggttt ggcaaggccg tgaagcgccg gccgatgcgt
     4321 cttgcatcag tgccttgaag catgccggtg ggcgtgttat aggcaaaacc gtcaccaccg
     4381 agttcgccta tttcacccct ggcgctactg ccaatcccca caatctggcc cataccccgg
     4441 gtggatcttc tagtggttcg gccgcagcgg tggctgcagg tatggtcccg gtcgcgctgg
     4501 gctctcaaac ggccgcttcg ctcattcgac ccgcagcgta ttgtggggtc gttggttatg
     4561 tggcttctgt tggctcggtg tcgcttcggg gagtgatgcc cctggcacat actttggatg
     4621 ccttgggtct ggtcacacgc gacgtcgccg atgtgcacta cgtgcaggcg ttattgacaa
     4681 gggctccagt gaagcagggg cgtgtccgtc gaaagccgga ggctgtattg gctgtcgatg
     4741 gcagggcatt tggggcggtc gatgcggaga tgctcgccct ccatcagcgc acgctagacg
     4801 gattggcatc gcaaggagtc aggatataca cggtgaccga cgagcttggg gccgagggag
     4861 gtcaaaaact ggcccaatgg caccatgagc ttatggcgta tgaagccgct caaacgctgt
     4921 cgttcgagta tcacgagcac gccgatgggt taagtcgcgc actacaagac ttgatcgaag
     4981 agggactcgg cattgatggt gagcgttacg aggaactgaa gtcgctgcgt gacatggcca
     5041 tggctaagtt tgcaagggca atggaccggt acgacgccgt attagcacct gctgcgccag
     5101 gtgctgcacc acgaggactt gaggcaaccg gtcggcctga ccagagtcgg gcttggcagc
     5161 tgttgggtgt cccacaaatc actctgccgg ctggcgtgga tgacgcttca atgccattgg
     5221 gcatgcaact cattgggcgc cagcgtggcg atgtacgact cttagacatc gcgctttggt
     5281 ttcaggatga gctgggctgg aagggcgaga tcccacctgc ctttcgccga cagtaactgt
     5341 ttattaacca cactctggtc cgttgatgtg cggccagggt tttcaagaga actgatatgg
     5401 acaccgtcgt tatcactcca cgctccggtt tggatatgca cgtttttggc tcgacgcgac
     5461 aggtcagtgg cgaggctatc cggacgttcg cgttgggaaa accgtatgtc acgtttgagc
     5521 atgttcaaaa gacttacgac ggcaactcac tggtagtgcg cgatctcaat ttgcaagtgg
     5581 cgaagcatga atttttaacg atgctagggc cgtctggctc cgggaaaaca acctgcctga
     5641 tgatgttggc agggtttgaa accgccacct caggcgaaat tttgcttgat ggtcgaccgg
     5701 tgaaccgcat tgcgcccgag caccgtgaca tcggtatggt gtttcagagc tactcactct
     5761 ttcctcacat gtcggtaagc cagaacctcg cgtttccctt gcgtattcgc aaggtgcctc
     5821 gacacgatat cgagaaacgg gtcaagcgtg cattggagat ggtcaacttg ggcgcgtttg
     5881 gtgaccgcat gccagcgcag ctctccggtg gtcagcagca acgggtggcc gtggcccgtg
     5941 ccttggtgtt cgagcccaag ctggtcctga tggacgagcc tttgggggca ctggacaaac
     6001 aactgcgcga gcagatgcag tacgagatca agcgtctgca caaagaactt ggcctgacgg
     6061 tggtgtatgt aacacatgac caggacgaag cgctcactat gtctgatcgc atcgctgtgt
     6121 tcaaggacgg tgtcattcag cagttggcgt ctccgacgga tctgtatgag cgccctacga
     6181 atgcgtttgt ggcgagtttc attggcgaaa acaacgagtg gtcggtgacg gttactgccc
     6241 gcaaggccga tctttgtgat gtgcgaaccc aggggggcgt aacccttgtc gcgctcaacg
     6301 tcgctgattt gaagcagggc cagcaagcgg tgctgtccgt gcgtcccgag cgcttggttt
     6361 tcgatgccga cttccgtgag tcggcgaatg catgcaacgt gatagccgca caggtgatgg
     6421 acgtgtcgta ccacggcgat cacctgcgtg tgcaatgtgc gttgaatggt ggggatgagt
     6481 tgatcgctaa ggttcccaac ggaggaacgg cgatgtcgct ggagaaaggg caaaaggtcg
     6541 cagttcattg gcgcgttgag gatgggcgcg tgctgtcgta gcgagccagg atctcaggag
     6601 ggtgttgatt gaacggactg agttccgtag gtattaattt ctgtttataa atctgaagga
     6661 gacaaatatg aaaattcgta aaaaggcgtt ggccctggca gccatcgtct cgctcacact
     6721 ggtggctcat ggcgtcaacg ccgccgatct gactttcgtg tcctggggtg ggacctacgg
     6781 gaagagccag gccgaagcac tcgtcaagcc gtattccgcc aagacagggc atcgaatctt
     6841 ggtggaagac tacagcggtg gattggctca aatccgcgca caggtcaagt ctaacaaggt
     6901 cacttgggac gtcgtggatc tggagttgca ggatgcgatt cgagcttgcg acgagggact
     6961 actggcaccg atccaagccc gcgatctgca aggcgttccc ggtgtggcgt cggtggtgga
     7021 tgacttcctg cccaactcgt tgaccccgtg ctcggtcggt accgtgatgt gggccaacgt
     7081 gatcaccttt aacctcaaca agtttgttgg ttccgagccg tctacggtgg gtgatttctt
     7141 tgatttgaag aaatttccag gaaaacgcgg catgaagaag gccgccaaag cgaatctgga
     7201 gtgggcgctg atggctgatg gcgtggcacc cgccgatgtc tataaggtat tgggtacgcc
     7261 cgccggtttg gatcgggcat ttcgcaagct caacacgatc aagtccagca tcgtatggtg
     7321 ggaggcgggt gcacaaccgc cccagttgct ggcagatggt gctgtgacta tgacttcggc
     7381 ctacagtgga cgtatccagg cggccattga tgtcgaccgc aagcctttca aaatcatttg
     7441 gcaggggcaa attcctgaat acgagacgct agcgatttta aagggaagca agaatgaagc
     7501 gacggcgcgt gacttcatca agttcgcgtc gtctccagcg gtgcttgccg aacagaccaa
     7561 gtacatcgcc tacggtccgc tccgcaagtc tgcgttgagt ctgatcgagt cttctgttgt
     7621 gtcgaagctc cccagcgccg ccgccaatat cgctgtgggt gtgccgtcca accctgaatg
     7681 gtgggccaac aacggtgatg agattaacca gcgcttctca gtctggctgg ccaactaaag
     7741 cggtctggcc atgggggtcg gtccgctgag ccccatgtag tccaggtggt cgagagctcg
     7801 ttgattcaag gatgattttg tggatatttc aattaacaac tcagttgagc tgaagcggca
     7861 gttgcgttcg acgcagcggg cgtttcgtct gcgtacgttc atgctggtga tgcccttgct
     7921 ggtgtttttg ctactcagtt ttttggcgcc tattggggtc gtcctctata agagcatcaa
     7981 cgacgacgtc atgacgggaa tcatgccgca gtcggcggca gccttgcgtg agtgggatgt
     8041 gcgtgcaaca tccttgcccc cccaaggaac acgtgatctc gtcgcacgtg aacttcaggc
     8101 tgcggcgaaa tccagggaga ttagtcgcgt cgcgacacgg gccaactttg aacgcccggg
     8161 catgcgcagc ctgcttatgt ccacctcacg caaattgatg gcgctggaag gtacccccga
     8221 ctatgaagcg ctaattgctg cagatgggcg gtggggcgaa acggaccctt gggtcgggtt
     8281 gaagtacatc agcagcgact tcacgtatca ctactatttg aacgccttgg atctgcgaca
     8341 agccgatgac ggttcgatca catcgcaacc ggatgacctc aagctctaca gtgacgtgtc
     8401 ttggcgcacc ttttccatca gtttccaagt agcgggtctg tgcctgttgc tgggttttcc
     8461 acttgcctat tttctagcga atctgccgag tcgtaccagc aacatgctga tggtctttgt
     8521 cctgctgccg ttctggacgt cgttgttggt ccgtaccatg gcatgggtcg tgatcctgca
     8581 gccggaggga gtggtgaact cggcgttgat gtccatggat gtgctatcag accccttacc
     8641 attgattttc aaccgatttg gtgtgttggt ggcgatggtg cacatcctga tgccctacat
     8701 ggtactgacg ttgtatagcg tcatgaaggc gattcctatg ggttacgtca gggccgctcg
     8761 ttccttgggg gccggtccgg tcatggcgtt tttcaatgtg tatttgccac aaacttttcc
     8821 tggtgtgttt gccggtacgt tgttggtctt tatccttgcg ctcgggttct atatcacgcc
     8881 ggcgatcgtg ggtggtgcac aggatcaaat gcttagcttc tttattgccg atcacatgaa
     8941 taactccctc aattggggct tggcttcagc cttgagtggt cttttgttgg tcggtgtttt
     9001 cattctgtat ggcctgtacg cacggctgac catgcgtaaa ggagagaaat gatgatgttt
     9061 cctgcatatg ttagctggaa aggcaagact tgccacctcg cctatcgggt gttttgtggg
     9121 ttgctgttgg ccttcctggc gttgcctgtg ttggtaaaca ttcctctgtc gttcaactcc
     9181 gagccgtatt tcagctatcc cttgagcgga ttcagcctga agtggtacca ggagttgctg
     9241 aatgagccac aatggctgtt ggccttgaaa aacactctca tcgtgggcgt cgcttcaaca
     9301 tcgattgcca cggtcttggg tgtgacggct gccctgggac tggcagagcg cgacctgccc
     9361 atgcgccata tcgtcaaggc gatcttcatc tcgccgatgg tggttcccgt ggtgacggct
     9421 ggcgtgggta tgtattttct ctactcgaaa tttgatctga cgggcaactt gcttggtttg
     9481 atcattgctc acgccgcact ggggactccc ttcgtggtca tgactgtcga ggccaccttg
     9541 gtaggttaca acgccagcct gacacgtgct gcccgcagtc tgggtgcatc tccgctctat
     9601 gccttcttca aggtcaagct gcccctcatt gcgccaggcg tgatttccgg ggcggtgttt
     9661 gcctttggta cgtctttcga cgaggtggtc gtggtattgt tcctgggggg ggttgagcag
     9721 cgcaccgtgc cgcggcaaat gtggtcgggt attcgcgagc agttgtcccc caccatcctc
     9781 gctgcagcca cggtcttgac ggtgttatcc gtcctcttgc tagtggtcct ggagatcctg
     9841 cgacgacggg gggaacagat gcgcggtgcg gtttcccact gagtcggagg ctgggtcatg
     9901 aggcgttcga ccgggcagca accgactcgc aacatcctgg gtatcggcgc catggtctta
     9961 ggcgttgcgt tgttcgcttg ggtcgacacc atcctaaagc tcttggttga atactatccc
    10021 ccgctccagg tggccggatt gagaggattg gtggcgctgc cgattctgtc ggtctggatc
    10081 cactggcgcg gcgcatgggg ctctttattc cggatacggg tgcgccttca ggggtcgtct
    10141 cagaaaacgg aaaataaagc acgttaagcc ggtggcagtg gtcgcaacgg tctgaacttg
    10201 cctgcgccga ccttggtgct gctgcgccag aggtaatcgc cggtcagatt gatgtgttcc
    10261 caccccagcg gtgacaggta ttgaaacagc gtctcgtcta ccggtttgcc gtgcccgttc
    10321 aaagcattgg ttgcccgttc cagataggcc gtgttccaaa gcacgatggc cgccgttacc
    10381 agattgaggc cgctggcccg gtagcgttgt tgctcaaaac tgcggtcgcg gatttc
//
LOCUS       DAOSLL010000081        10418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6264_length_10418_cov_4.421307, whole genome shotgun sequence.
ACCESSION   DAOSLL010000081 DAOSLL010000000
VERSION     DAOSLL010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10418
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6264_length_10418_cov_4.421307"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="PLB25_05245"
     CDS             <1..953
                     /locus_tag="PLB25_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE41022.1"
                     /translation="GGIWWSLVEKYKVTVMFSAPTAVRVLKKQDPALLKKYDLSSLRA
                     LFLAGEPLDEPTAQWISDGLGKPIIDNYWQTETGWPILSLCNGVEQAKSKFGSPGKAV
                     YGYDVKLLDDQTGAELIGPNQKGVVVVEGPLPPGCMQTVWRGDDRFVSTYWKTVPGKM
                     VYSTFDWGIRDADGYYFILGRTDDVINVAGHRLGTREIEESISAHPNVAEVAVVGVAD
                     QLKGQVAMAFAVAKDASLVADAAGAAKLEAEIMKMVDKDLGAVARPARVRFITVLPKT
                     RSGKLLRRAVQAICEGRDPGDLTTMDDPAALQQIKDLISS"
     gene            1141..4008
                     /locus_tag="PLB25_05250"
     CDS             1141..4008
                     /locus_tag="PLB25_05250"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HOE41023.1"
                     /translation="MSETHTVYQAYQANTYLFGGNAPYVEEMYENYLANPGSVPDTWR
                     GYFDALQHVPAADGSNAKDVPHQPVIDAFAQRAKAGGTRVVVASADSEMGRKRTAVQQ
                     LIAAYRNVGASWADLDPLKRTERPNIPELEPSFYGFADADQETVFDISNTFFGKDKMS
                     LRELLNALRETYCGTIGAEYTYLTDQTQKRWWQQKLESVRSKPNFDVRQKKHILDRLT
                     AAEGLERFLHTKYVGQKRFSLEGGESFIAAMDELIQQAGAKGVQEIVIGMAHRGRLNV
                     LVNTLGKMPADLFAEFDHTAPEDLPAGDVKYHQGFSSDVTTPGGPVHLSLAFNPSHLE
                     IVNPVVEGSVRARMDRRSDPTGRQVLPVLVHGDAAFAGQGVNQETLALAQTRGYTTAG
                     TVHIIINNQIGFTTSDPRDYRSTLYCTDIVKGVEAPVMHVNGDDPEAVVMAVQWALDY
                     RMEFRKDVVLDIVCFRKLGHNEQDTPALTQPLMYKKIGQHPGTRKLYADKLAAQGLGD
                     ELGNDMFKAYRAAMDEGRHTSDPVLTNFKSKYAVDWAPFLGKKWTDAGDTAVPLAEWK
                     RLAERITTIPPTVTPHGLVKKVYDDRAAMGRGDIPVDWGMGEHMAFASLVASGYPVRL
                     SGEDCGRGTFTHRHAVIHDQKREKWDIGTYVPLQNVADQQAPFVVIDSILSEEAVLGF
                     EYGYASNDPNTLVIWEAQFGDFANGAQVVIDQFIASGEVKWGRVNGITLMLPHGYEGQ
                     GPEHSSARVERFMQLAADTNMQLVQPTTASQIFHVLRRQMVRHLRKPLVIFTPKSLLR
                     HKDAASPLSEFTDGGFQTIIPEHKALKNDKVKRVIACSGKVFYDLSKKREERGIEDVA
                     ILRVEQLYPFPHKTFATELKKYPNATEVVWTQDEPQNQGAWFFVQHYIQENMLDGQKL
                     GYSGRAASASPAAGYAHLHQEQQKALVEGAFGKLKGFVLTK"
     gene            4100..5356
                     /gene="odhB"
                     /locus_tag="PLB25_05255"
     CDS             4100..5356
                     /gene="odhB"
                     /locus_tag="PLB25_05255"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HOE41024.1"
                     /translation="MALVEVKVPQLSESIAEATLLQWKKKVGEAVAVDEILVEVETDK
                     VVMEVPAPSAGVIVEIVQPDGSTVAAEQLIARIDTDGKAGAVAAPVPQAAAVAAPVAA
                     AQLAVASKAGVAMPSAAKLMADNQLAAGSVAGTGKDGRVTKGDVLGVLAAPKNVAAPA
                     MPARAAAVSLPQVAVPVGKLADDLIGRPEQRVPMSRLRARVAERLLQSQSTNAILTTF
                     NEVNMAPVMEMRKRFQDKFEKEHGVKIGFMSFFVKAAVHALKKFPVLNASVDGNDIVY
                     HGFFDIGIAVGSPRGLVVPILRNADQMSFADIEKKIAEYGAKARDGKMSMEEMTGGTF
                     SISNGGTFGSMMSTPIINPPQSAILGVHATKDRAMVENGQVVVRPMNYLAMSYDHRII
                     DGREAVLGLVAMKEALEDPARLLFDI"
     gene            5504..6931
                     /gene="lpdA"
                     /locus_tag="PLB25_05260"
     CDS             5504..6931
                     /gene="lpdA"
                     /locus_tag="PLB25_05260"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HOE41025.1"
                     /translation="MNKQFDVIVIGAGPGGYIAAIRAAQLGKKVACVDAWRNSSGGPA
                     PGGTCTNVGCIPSKALLQSSEHYEHASHHFADHGIAVKGLSMDVAKMLARKDGVVKQN
                     NDGILYLFKKNKVSFFHGLASFVKAGQGGYEIKVAGAAEETLQGQQIIIATGSSARAL
                     PGAPFDEELILSNEGALCIGTVPKKLGLIGSGVIGLEMGSVWRRLGADVTILEGLPDF
                     LGVVDTQIAKEAQKAFAKQGLKIELGVTVGEVKRGKKSVSVAYTNAKGEALSLEVDKL
                     IISIGRLPNTNGLNAAAVGLQLDERGMIEVDEDCRTNLPGVWAVGDVVRGPMLAHKAE
                     EEGVAVAERIAGQHGHVDFSTIPWVIYTSPEIAWVGQTEQQLKASGRAYRAGTFPFLA
                     NGRARALGDTTGMVKMLADAATDEILGVHIVGPMASELIAECVMAMAFRASSEDIARI
                     CHAHPSLSESTKEAALAVDKRTLNF"
     gene            7012..8109
                     /gene="zapE"
                     /locus_tag="PLB25_05265"
     CDS             7012..8109
                     /gene="zapE"
                     /locus_tag="PLB25_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HOE41026.1"
                     /translation="MTVKKAYQAELLARGFTADPAQLRAVQELNTCAREWAKFKEKRS
                     NSFKKLINRLPIPRGIYMYGGVGRGKSFLMDCFYGAVPLKRKTRLHFHEFMREVHREL
                     AGMQGTANPLDVLAKRISRKYRLICFDEFHVSDITDAMILHRLLVALFENGVGLVTTS
                     NFRPDDLYPNGLHRDRILPAIALLNARLTVINVDNGTDYRGRTMEQLQLYYTPLDDAA
                     DAAMLDAYERLAESHDEDPVLQIEARKIHAKRKAGALVWFDFKALCGGPRSQNDYLEI
                     ATQFHTVLLSGVPLLPPRMSAEARRFTWLVDVLYDRRVKLIMSAAVPVEQLYTEGQMA
                     NEFARTVSRINEMQSAEFLALDRRDVDTSLT"
     gene            8163..8498
                     /locus_tag="PLB25_05270"
     CDS             8163..8498
                     /locus_tag="PLB25_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41027.1"
                     /translation="MQLFAQEALAAKTVQVELERRRIGAERLQQELLYSESEKACFQR
                     FAVKDCLRQASAARRLVLNELRRQELVLNDLERQAKASEATNRTQKKRADQQAKEPES
                     LPGTTLRPD"
     gene            complement(8517..9290)
                     /locus_tag="PLB25_05275"
     CDS             complement(8517..9290)
                     /locus_tag="PLB25_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOE41028.1"
                     /translation="MTAKGHRLTAATGIHKGDRDYQQDQVTLLMHPRAKGCLMAILAD
                     GMGGRSGGRKASDQVMLTGRQLFERYSPGADDPVATLKQLVMEAHTVIKLTAISSEQE
                     PHSTVASFLINPDGSCHWVHAGDSRIYFFRDRTLVKRTLDHSYVQTLVNQGKITPEEA
                     NFHPRSNILMGCLGTDKDPILDIDFIPCLLPGDILLVCSDGVWHYFNDIELGSVLTTP
                     SARDASEFLIEKARSRARGSGDNLSLAIVKFEALDAPSP"
     gene            complement(9295..10101)
                     /locus_tag="PLB25_05280"
     CDS             complement(9295..10101)
                     /locus_tag="PLB25_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HOE41029.1"
                     /translation="MPGYKLSTVFRLVLAGGLLLLGACSTNKTDPTTGWSPNKIYAEA
                     KDELNSGGYEKAIPLFEKLEGRAAGTPLAQQAQIDKAYAQYKNGDAVQAVATLNRFMK
                     LHPASPAIDYALYLKGVVNFNDDLGLFSAITLQDLSERDQKAAKESFEAFKELVARYP
                     DSRYAPDARARMIYVVNALAQSEVHVARYYFERGAYLAAINRAQTAIGEYQGVPAAEE
                     ALFIMVKSYDALSMPQMRDDTLRVLEKNYPQTPYLTQGFKGKIQPWWKLW"
     gene            10116..>10418
                     /locus_tag="PLB25_05285"
     CDS             10116..>10418
                     /locus_tag="PLB25_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOE41030.1"
                     /translation="MIKTETIISTPLTLLGLDSAELADGEIERRELLFGPEHHNQRID
                     RALADLIPEFSRSYLQQMIDSGGVSVNGQVVGKSSLRVKLADRGSIELRPTPQSHSF"
BASE COUNT         2273 a         2838 c         3074 g         2233 t
ORIGIN      
        1 acggcggcat ctggtggagc ctggtggaaa aatacaaggt cacggtcatg ttcagcgccc
       61 cgacagcggt gcgcgtgctc aaaaagcaag acccggccct gctgaaaaag tacgaccttt
      121 cgagcctgcg tgcgctgttc ctggcgggtg aaccacttga cgagccgacc gcgcagtgga
      181 tcagtgacgg cctgggcaag ccgatcatcg acaactactg gcaaaccgaa accggctggc
      241 cgattttgtc gctgtgcaat ggtgttgaac aggccaagag caagtttggc tcgcccggca
      301 aggccgtcta tggctacgat gtcaaactgc ttgatgacca gaccggtgca gaactgattg
      361 gcccgaatca aaagggtgtg gtggtggtcg aaggtccgct gccgcccggc tgcatgcaaa
      421 ctgtctggcg cggtgatgac cgttttgtca gcacctactg gaagacagtg cccggcaaga
      481 tggtctacag cacttttgac tggggcattc gtgatgctga tggttactat ttcatcctgg
      541 gccgtaccga tgacgtgatc aatgttgccg gtcaccgtct gggtacccgc gagattgagg
      601 aaagcatctc ggcgcacccc aatgtggccg aggtggccgt ggtgggcgtg gcagaccagc
      661 tcaaagggca ggttgccatg gcgtttgcgg tagccaagga tgccagtctg gtcgctgatg
      721 cggcaggtgc tgccaagctg gaggccgaga tcatgaaaat ggttgacaaa gacctcggcg
      781 cggtggcacg accggcccgc gtgcgtttca tcaccgtttt gccaaaaacc cgcagcggca
      841 aactgttgcg ccgggccgtg caagcgatct gcgaaggccg tgaccccggt gacttgacca
      901 ccatggacga ccccgcagcg ctgcagcaaa tcaaggattt gatcagctcc tgagcgtttt
      961 ctgcatcttt gccgcgtaag gactgtgcca tcttcgggtg gcacaatctg cggcccaatc
     1021 aggccccttc caacgccaca gtcgcatccg ctaaacggtc aagccgtgac gcgggaggtt
     1081 aatcaactac cagctttaac cagcagcagt ttccccaagg gaagcgaaag tcagcgaaac
     1141 atgagtgaaa cccacacggt ttaccaagcc tatcaggcca atacttacct ttttggtggg
     1201 aacgccccgt atgtcgaaga gatgtacgaa aactacctgg ccaatccagg tagcgtgccg
     1261 gatacctggc gcggctattt tgatgcccta caacatgtcc ctgccgctga cggcagcaac
     1321 gccaaagacg ttcctcacca gccggtgatt gacgcgttcg cccagcgagc caaagccggt
     1381 ggtaccaggg tggtggtggc cagtgctgat tccgaaatgg gtcgcaagcg cacagcagtg
     1441 cagcagctca ttgctgcgta tcgcaatgtg ggtgccagtt gggctgatct tgatcccctc
     1501 aaacggaccg agcgcccgaa cattcctgag ctggagccct ctttttatgg tttcgctgat
     1561 gccgatcagg agaccgtgtt cgacatcagc aacacttttt ttggcaaaga caaaatgtct
     1621 ttgcgcgaac tgctcaatgc gctgcgcgaa acctactgcg gcacgattgg tgccgagtac
     1681 acctatctga ccgatcagac gcagaagcgc tggtggcaac aaaagctcga aagcgtgcgc
     1741 agtaagccaa actttgatgt gcggcagaaa aagcatattc ttgaccgctt gacggcggca
     1801 gaaggtctgg agcgcttttt gcacaccaag tatgtgggtc aaaagcgatt ttctctggaa
     1861 ggtggcgaaa gcttcatcgc tgccatggac gaacttatcc agcaggcagg ggccaaaggc
     1921 gtgcaggaaa tcgtgattgg catggcacac cggggtcgtc tcaatgtgct ggtcaacacg
     1981 cttggcaaaa tgcccgccga cctgtttgcc gagtttgacc acacggcacc tgaagatttg
     2041 cctgcaggtg atgtcaagta ccaccagggt ttcagttcgg atgtcaccac ccccggcggc
     2101 ccggtgcacc tcagtctggc ttttaacccg tcgcaccttg aaattgtcaa ccctgtggtg
     2161 gaaggctcgg tgcgggcccg gatggaccgc cgctctgacc cgaccggtcg ccaggtgctg
     2221 ccggtgctgg tgcatggcga tgccgccttt gccggtcaag gggttaacca ggaaaccctg
     2281 gcactggcac aaacgcgtgg ctacaccacg gcgggcacgg ttcatatcat catcaacaac
     2341 cagattggtt ttaccacctc tgatccgcgc gactaccgct cgacgctgta ttgcaccgac
     2401 atcgtcaaag gcgttgaagc gcctgtcatg cacgtcaatg gtgacgaccc tgaggctgtg
     2461 gtgatggcgg tgcagtgggc tctggactac cgcatggagt ttcgcaagga cgtggtgctt
     2521 gacattgtct gtttccgaaa gctcggccac aacgagcaag atacaccggc gctcacccag
     2581 cccctgatgt acaagaagat cggtcaacat ccgggcaccc gcaagctgta cgccgacaag
     2641 cttgcagcgc aaggcctggg cgacgaactt ggcaacgaca tgttcaaggc ctaccgcgct
     2701 gccatggatg aaggtaggca cacttcagac ccggtgctga ccaatttcaa aagcaaatac
     2761 gcggtcgatt gggccccttt tctgggcaag aaatggaccg acgcaggcga taccgcagtt
     2821 ccgctggccg aatggaagcg cctcgccgaa agaattacca ccattccgcc aacggtaacg
     2881 ccgcatggcc tggtcaaaaa ggtctatgac gaccgcgccg ccatggggcg tggcgacatc
     2941 ccggttgatt ggggcatggg cgaacacatg gcgtttgcct cgctggtggc cagtggttat
     3001 cccgtgcgcc tgagcggtga agattgcggc cgcggcactt ttacccaccg gcacgcggtc
     3061 attcatgacc aaaagcggga aaagtgggac atcggcactt acgttccgct gcaaaacgtg
     3121 gctgaccaac aggcaccttt tgtcgtcatt gattcgatcc tgtcggaaga ggcggtgttg
     3181 gggtttgaat atggctatgc gtccaatgac cccaacacgc tggtcatctg ggaagcccag
     3241 tttggcgatt ttgccaatgg cgcacaggtg gtgattgacc agttcattgc gtcaggcgag
     3301 gtgaaatggg gccgcgtcaa tggcatcacc ctgatgcttc cgcacggtta tgaggggcag
     3361 ggcccggagc attcatcggc ccgggtcgag cgcttcatgc aactggctgc cgataccaat
     3421 atgcaactgg tgcaacccac cacagccagc cagattttcc acgtgctgcg ccgccagatg
     3481 gtgcgccacc tgcgcaaacc gctggtcatc ttcacgccca agtcgttgct gcgccacaaa
     3541 gatgccgcgt cgcccttgtc cgagttcacc gacggtggtt ttcagaccat catcccggaa
     3601 cacaaggcac tcaagaatga caaggtcaag cgcgtgatcg cctgctcggg caaggttttc
     3661 tacgatctgt cgaaaaagcg cgaggaaagg ggcattgaag acgtggcgat cttgcgcgtt
     3721 gagcagttgt acccgtttcc ccacaagacg tttgccactg aactcaagaa gtaccccaac
     3781 gcgacggaag ttgtgtggac gcaagatgag ccgcaaaacc agggtgcctg gttttttgtg
     3841 cagcattaca tccaagagaa catgcttgac gggcaaaagc tggggtattc cggtcgtgcc
     3901 gcatcggcct cacctgcagc aggctatgcg cacctgcatc aggaacaaca aaaagcgctt
     3961 gttgaaggcg cttttggcaa gctcaaaggc ttcgtgctga caaagtaagg gccgcaattc
     4021 ctctggtcag accgttcaag gcgggcagcg cagcccgtct tctggacacc ccccaagcaa
     4081 acattttgaa agaattgata tggcactcgt agaagtcaaa gttccgcagc tctcggaatc
     4141 gatagcagaa gccacgctgt tgcaatggaa gaagaaagtt ggcgaagcag tcgctgttga
     4201 tgaaatcctg gttgaagttg aaactgacaa ggtagtgatg gaagtgccag ccccgtccgc
     4261 cggcgtgatt gtggaaattg tgcagcccga tggcagcacc gtcgccgccg aacaactgat
     4321 cgcccgcatc gacactgatg gcaaggcggg tgcagtggca gcgcctgtgc cccaagcggc
     4381 cgcggtcgcc gcaccggtgg ctgcggcgca gcttgctgtt gcatccaaag ctggtgtggc
     4441 aatgccgtcg gcagccaagc tgatggccga caaccaactc gccgctggtt ccgtggctgg
     4501 caccggcaag gacggccggg ttaccaaggg tgatgtgctg ggtgtcctgg cagctccaaa
     4561 aaatgtagct gcgcctgcaa tgccggcaag ggctgcagcc gtttctttgc ctcaagttgc
     4621 agttccggtt ggcaaactgg cagatgactt gatcgggcgt ccagagcaac gcgtgccaat
     4681 gagccgcttg cgtgctcggg tggctgagcg cctgctgcaa tcgcaatcga ccaacgccat
     4741 tctgaccacc ttcaacgaag tcaacatggc cccggtgatg gaaatgcgca agcgcttcca
     4801 ggacaagttc gagaaggagc acggtgtcaa gattggtttc atgagctttt ttgtcaaggc
     4861 tgcggtgcat gcgctgaaga aatttccggt gctcaacgcg tcggttgatg gcaatgacat
     4921 cgtctatcac ggcttcttcg acattggcat tgcagtgggc tcgccgcgcg ggctggtggt
     4981 accgattttg cgcaatgccg atcagatgag ctttgccgat atcgagaaga aaatagccga
     5041 atacggtgcc aaggcgcgcg acggcaagat gagcatggag gaaatgaccg gcggcacctt
     5101 ctcgatcagc aacggtggca cttttggctc gatgatgtcc acgccgatca tcaacccgcc
     5161 gcaaagtgcc attttggggg tgcatgcgac caaggaccgt gccatggtgg aaaacggcca
     5221 ggttgtggtc cggccgatga actacctggc catgtcgtat gaccaccgca tcattgatgg
     5281 ccgtgaagcg gtgctcggac tggtggccat gaaagaagcg ctggaagacc cggcgcgtct
     5341 gctgtttgac atctgattga taagaaattg gccggtagcc cccgtcctgt aagcgtgggc
     5401 tgctattgtt ttaggtattg tccgttgccg ggccaacttg atctggtgtc gatgcgccgg
     5461 ttttggcaac aggtttgtag acaaattttg atgcggactt tttatgaaca agcaatttga
     5521 tgtgatcgtc attggtgctg gccctggcgg ttatatcgcc gccattcggg ctgcccaact
     5581 tggcaaaaaa gtggcctgtg tggatgcctg gcgcaacagt tccggcggtc cggcaccggg
     5641 cggcacctgc accaatgtcg gttgcatccc gtccaaggcc ttgttgcagt cctcagagca
     5701 ctacgagcat gccagccacc actttgccga tcacggcatt gcggtcaagg gcctgagcat
     5761 ggatgttgcc aaaatgctgg cccgcaagga cggcgtggtc aagcagaaca atgacggtat
     5821 tctttacctg ttcaagaaaa acaaggtcag cttttttcac ggcctggcct cgtttgtcaa
     5881 ggcaggtcag ggcggctatg agatcaaggt ggctggtgct gccgaagaaa cgctgcaggg
     5941 gcagcaaatc atcatcgcca ccggttcttc ggcgcgggcc ttgcccggtg caccgtttga
     6001 cgaagagctg attttgtcca acgaaggcgc attgtgcatc ggcaccgtac cgaaaaaact
     6061 gggcttgatt ggctcgggcg tgatcgggct ggaaatgggc tcggtgtggc ggcgcctggg
     6121 tgccgacgtg acgattctgg aaggcttgcc agattttctt ggagtcgtcg atacgcagat
     6181 cgccaaagag gcgcaaaagg cgtttgccaa acaaggcttg aaaatcgaac ttggcgtgac
     6241 cgttggcgag gtcaagcgtg gcaaaaagag tgtcagtgtc gcctacacca acgccaaagg
     6301 cgaggcgctg tcacttgagg ttgacaagct gatcatttca atcggtcgcc tgcccaatac
     6361 caatggcctg aatgccgcag cggtgggtct gcagctcgat gagcgcggca tgatcgaagt
     6421 cgatgaagac tgccgcacca atttgcctgg tgtctgggcg gtgggggatg tggtgcgcgg
     6481 cccgatgctg gcgcacaagg ccgaagaaga aggtgtggcg gtggctgaac gcatagcagg
     6541 ccagcatggc catgttgact tcagcaccat tccctgggtg atttatacca gcccggaaat
     6601 tgcctgggtc ggccagaccg agcagcagct caaggcgagc ggacgcgcat accgggcagg
     6661 tactttcccc ttcctggcga acggtcgcgc acgcgcgcta ggcgacacca ctggcatggt
     6721 caagatgctg gctgatgctg ccaccgatga gattttgggc gtgcacatcg tcggcccaat
     6781 ggccagtgaa ctgattgccg aatgtgtgat ggcgatggcg tttcgtgcca gcagcgaaga
     6841 catcgccaga atttgccatg cccacccgtc tttgagtgaa tcgaccaaag aggcggctct
     6901 ggcggtggac aagcggaccc tgaatttctg aaggaatata taagaaatgg ggctctagcc
     6961 gttgtcctat atgcgccaac agctacttaa tacatagcat ttgagaatcc tgtgaccgtt
     7021 aaaaaagctt atcaagccga attgcttgcc cgtggtttca cggctgaccc cgcccaattg
     7081 cgggccgtac aggaactcaa tacttgcgcg cgggaatggg ccaagttcaa ggagaagcgt
     7141 tccaattcgt tcaaaaagct gatcaaccgt ctgcctattc cgcgtggcat ttatatgtac
     7201 ggcggggtag ggcgaggcaa gagttttttg atggattgct tttacggggc agttccgctc
     7261 aagcgtaaaa cccgcctgca ttttcacgag ttcatgcgcg aggtgcaccg cgaactggcc
     7321 ggcatgcagg gcaccgccaa tccgctggac gtgctggcca aacgcatctc gcgcaaatac
     7381 cggttgatct gctttgacga gttccatgtg tcggacatta ccgatgccat gatcttgcac
     7441 cgactgctgg tggccctgtt tgaaaacggt gttggcttgg tcaccacgtc aaacttcagg
     7501 cctgatgact tgtaccccaa tgggttgcac cgtgaccgga ttctgccggc cattgccctc
     7561 ctcaatgcgc gcctgacggt catcaatgtt gacaatggca cggattaccg tggccgcaca
     7621 atggagcaac tccaactgta ttacacgccg ctcgatgatg ctgccgatgc ggccatgctc
     7681 gatgcttacg agcgtctggc agaaagccac gacgaagatc cggtgctgca gattgaagcg
     7741 cgaaaaatcc acgccaagcg caaggctggc gcgttggtct ggtttgattt caaggcgctt
     7801 tgtggcgggc cccgatcgca aaacgactat cttgagattg ccacccagtt tcacacggtc
     7861 ctgctcagtg gcgtgccgct gctgccgcca cgcatgtcgg ccgaggcgcg gcgttttacc
     7921 tggctggtcg atgtgctgta tgaccggcgc gtcaagctga tcatgtcggc ggctgtgccg
     7981 gttgagcagt tgtacaccga agggcaaatg gctaacgagt ttgcacgaac agtgtcgcgt
     8041 atcaatgaaa tgcagtcggc tgaattcctg gcgctggacc ggcgcgatgt tgataccagt
     8101 ttgacctgat gcacagggca cttttgctta acgtcgcttt gttgtgcggc ctcgcggcac
     8161 ctatgcagct tttcgcccaa gaagcgcttg cagcgaaaac agtacaagtg gagcttgaac
     8221 gtcggcgcat cggggccgaa cgcttgcaac aagagttgct ctacagtgaa tcagagaagg
     8281 cctgttttca gcggtttgcc gtgaaagatt gcttgcgcca agcgagtgcc gcgcgccgcc
     8341 tggttttgaa tgaactgcgt cggcaggaac tggtgttgaa cgatctggag cgtcaagcca
     8401 aggcatcaga agcgaccaac cgcactcaaa aaaaacgtgc ggatcagcag gcgaaggaac
     8461 cagaaagttt gcctggaacc acgttgcggc ccgattgagc ctggtggtct tggcattcac
     8521 ggcgacggtg cgtcgagtgc ctcaaacttc acaatcgcca gtgacaggtt gtcgccgcta
     8581 cccctggctc tgctgcgggc cttttcaatc aggaattcac tggcatccct ggccgacggg
     8641 gtggtcagca cggagcccag ttcgatgtca ttgaaatagt gccagacccc gtcgctgcag
     8701 accagcagta tgtcgcccgg cagcaagcag ggaatgaagt caatgtccaa aatcgggtct
     8761 ttgtcagtgc caaggcagcc catcaagatg ttggaacgcg ggtggaaatt ggcctcctct
     8821 ggcgtgatct tgccttggtt gaccagagtc tgaacgtagg agtgatccaa cgtgcgtttg
     8881 accaatgtcc tgtcgcgaaa aaaataaatc cgcgagtcac ctgcatgtac ccagtgacag
     8941 cttccgtctg gattgatcaa gaaacttgca accgtgctgt gtggctcttg ctcggaagaa
     9001 atggcggtga gcttgatcac cgtgtgtgct tccatgacca actgcttgag tgtggccacc
     9061 ggatcatccg ctccgggcga gtagcgctca aacagttgtc tgcccgtcag catgacttga
     9121 tcggaagcct ttcgaccacc acttcgaccg cccatgccat ccgccaggat cgccatcagg
     9181 cagcccttcg ctcgcgggtg catcaacaac gtaacttgat cttgttgata gtcccggtcg
     9241 cccttgtgaa taccggttgc ggcggtaagt cggtggcctt tggcagtcat aggctcacca
     9301 tagtttccac catggttgaa tcttgccttt gaatccctgg gtcagatatg gggtctgtgg
     9361 gtagttcttc tccagcacgc gcaaggtgtc gtcgcgcatc tggggcatgc tcagggcatc
     9421 gtaagacttg accatgatga agagcgcttc ctcggccgct ggcacgccct ggtattcacc
     9481 aattgccgtt tgtgcccgat tgatggccgc cagataggca ccgcgttcaa aatagtaacg
     9541 tgcgacatgc acctcggatt gcgccagcgc attcaccacg tagatcatgc gtgccctggc
     9601 atctggtgca taacgagaat caggatagcg cgccaccagt tccttgaatg cctcaaacga
     9661 ttccttagcc gctttttggt cacgctctga caagtcttgc agggtgattg ccgagaataa
     9721 tccgagatcg tcgttaaaat tgacaacgcc tttcaaatac agtgcgtaat cgattgctgg
     9781 gctggccggg tgcagtttca tgaaccggtt cagggttgcc acggcctgaa ccgcatcgcc
     9841 gtttttgtat tgggcgtaag ctttgtcgat ctgggcttgc tgcgctagcg gtgttcccgc
     9901 cgcacgacct tcaagttttt caaaaagggg aatggccttc tcgtagccac ccgaattcaa
     9961 ttcgtccttg gcttcagcgt aaattttgtt ggggctccag ccagtggttg gatcagtctt
    10021 gttggtcgag caagcaccga gcaacaaaag cccaccggct aaaaccaagc gaaagacagt
    10081 tgataatttg taaccgggca tgtaggacaa ctttcttgat caagaccgaa acgattatat
    10141 cgacccccct tactttgttg ggcctggatt cggccgaact ggcagacggt gagattgagc
    10201 gacgcgagtt attgttcggt cccgaacatc acaatcaacg aattgaccgg gcgctggctg
    10261 atttgattcc cgagttttcc cgcagctact tgcaacagat gattgactca gggggtgtca
    10321 gcgtcaatgg tcaggtggtt ggcaagtcat ccctgcgggt caaactggca gatcgcggca
    10381 gcattgagct cagaccgaca ccccagagtc attccttc
//
LOCUS       DAOSLL010000082        10412 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6266_length_10412_cov_4.907309, whole genome shotgun sequence.
ACCESSION   DAOSLL010000082 DAOSLL010000000
VERSION     DAOSLL010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10412
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6266_length_10412_cov_4.907309"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..3597)
                     /locus_tag="PLB25_05290"
     CDS             complement(172..3597)
                     /locus_tag="PLB25_05290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE41031.1"
                     /translation="MFFVLVAASIGGHSYVDYYATVDRELQAFEGHVRLADVQISSVL
                     QRTEMITQSVVTDELSSPKLPATAAHQRKLNLLQQFPEVDVLFVLDSMGRVVDAASIR
                     MPEVLPRLEGLDAAQQEYFLVHRNARVEEARRNWLSRPFKETGGRYIISLSKAIRGND
                     GVLQGVVVIFTAPQLFDNVLSELLRNGAIDAAAVHNRQGDIIYRLPNPSTYVGKNIGS
                     AESFKKYLASTEKMTRYVGVTATDGVKRVLVFGRVGDTTLDVAVSVKTDSLMVDWKNR
                     TLWKLLIFLAVALLMTLLAWQTQRRRVFREERERAERRFRAYFERSMVGMATIGSANE
                     LLDINQTLCEMLGFSASELHKKVWVDLVDASERPAVLAKSARLLAGELDEYQCEQVFV
                     HSTGKRLAVEVAVRAVRTRHKKLDFVVVLFNDVTTRKNAEVALIQAKVEAEQANVAKS
                     DFLANMSHEIRTPMNAIIGLSGLALKNEMPPRIQDYLSKIKQSGEHLLGIINDILDFS
                     KIESGKLEIESVPFELEAVIDNVVNLISQKAEAKHLELLCSFDNAVPRNLIGDPLRIG
                     QILINYANNAVKFTEHGELRISIRVLESTASEVLLHFALSDTGIGLKPEQMGRLFKSF
                     EQADSSTTRQYCGTGLGLAISKSLAESMGGEVGVQSEYGKGSTFWFTARLGIGSAEKI
                     ITRPSVDLHGRRVLVVDDNDTAALVLCELLQELGFVVQSVNSGAAALQAVAQAVAAGL
                     AFDFVMMDWQMPGMDGLEAVRQIQEMNPRSAPFVLMVTAHRRQELLKGAQRLGVEHVL
                     AKPVGASLLVDTMMQLEGHAPRKPANAPQGQAASAAEAALAPMAGARILLVEDDEINQ
                     MVACELLQIVGFAVDVADNGQIGVHQVHARFADAQPYDIVLMDMQMPVMDGVTASRLI
                     RESFTSEALPIVAMTANAMKADKDRCMAAGMNGFISKPIDPEELWRALLAWVKPRSGA
                     GPAQVPTVSAAPPQPQPQPQTKPLEQMLIALHEIVGLDASRGLTQCNRNPKLYVSLLG
                     KFVKAQEHTLENIRQALADGDAGTAQRLAHTLKGLCASMGAEPLRQTLLEIEQAVREG
                     RNTDDIEGLIVPASAELTTLFASLRGALGLNQGPAAARPAQAGLAAPEVATSA"
     gene            complement(3745..3942)
                     /locus_tag="PLB25_05295"
     CDS             complement(3745..3942)
                     /locus_tag="PLB25_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41032.1"
                     /translation="MPDIAIGEVDLSLRLKKDRRNTELSEITSRVKFFVGSCNWSFVS
                     SKSLSNEIDKFFNLARREDAA"
     gene            complement(4447..4821)
                     /locus_tag="PLB25_05300"
     CDS             complement(4447..4821)
                     /locus_tag="PLB25_05300"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOE41033.1"
                     /translation="MLEALIGDDAGTVDQFVADFYISAAMLTGELLGAYAAGKTSQVE
                     AQAHKLKLSARAVGALQLGELCAAMERTAKEQDAQALAVLVPAFEREAARVSGFNLNR
                     KVPHVSRQGPHLHAVALHEAPP"
     gene            complement(4824..5441)
                     /locus_tag="PLB25_05305"
     CDS             complement(4824..5441)
                     /locus_tag="PLB25_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE41034.1"
                     /translation="MAAALPGRALLEAVAIAAGRAKAPDRQIQPSLAPMAHTPMSHED
                     ARRQGSLILVAEDNEYNQKVMLQQLVLLGRTADIVNNGQEALARWQSGSYCLLITDLH
                     MPLMDGYQLIAAIREAEVGKSRPPIIAFTANVLKGEAEHCIDVGMDDYLSKPVQLTQL
                     RAILDRWQPEVVATPIDLANLSQQPETVIPFAAQAADADPACRWM"
     gene            5498..5827
                     /locus_tag="PLB25_05310"
     CDS             5498..5827
                     /locus_tag="PLB25_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41035.1"
                     /translation="MDAHIVGQAVGATENQAVQAGHLKSIGSHARRCTEACKRQPHAE
                     HRTPPQLAADRDVATIVMVFLPLIFGLRRTLFYRRVVFFGGSLLIVGVALVWLVERVF
                     DLTLISA"
     gene            6034..6177
                     /locus_tag="PLB25_05315"
                     /pseudo
     CDS             6034..6177
                     /locus_tag="PLB25_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464931.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate synthase"
     gene            6171..6488
                     /gene="dut"
                     /locus_tag="PLB25_05320"
                     /pseudo
     CDS             6171..6488
                     /gene="dut"
                     /locus_tag="PLB25_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464930.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
     gene            6488..6720
                     /locus_tag="PLB25_05325"
                     /pseudo
     CDS             6488..6720
                     /locus_tag="PLB25_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            7113..10163
                     /locus_tag="PLB25_05330"
     CDS             7113..10163
                     /locus_tag="PLB25_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE41036.1"
                     /translation="MRTIVALLLALGIAGLVLWARDQLVSAQRVERLQLETLAMASLV
                     ESVSNKGVVMSGASLIGVFDVRAKRMLSSAAVAMDLEALADFATVIGLLHVQGASILN
                     AIGVTQASLNAAGTFGGAGANFLQKPYIRKALSGVSAVNLEVDATTAERMLHFAAPVF
                     EERSTSSKVLGVYHLETNVAEVDKVLAKYLPRTALLVSPEGTVFASNRSVQDLMPLLA
                     QSGETAEKNARFGYLALRAQVPSGTNGLTRFEVKDFHVTNVKLGWPAEQGAWQTVLME
                     PSNDSAEMWSRLILGLSVFSVLFGSYSMLARHNAAQARFRQVLQAKQVEQEASLRAYA
                     DQVDFQACLMDAIPTPLFVKDAQGRTAGMNRAYEEAYGVRRQDYVGKTLLQTSLRNLD
                     AAQRALYFEEQIDIIASGALVHKKREMCWADGAIHHELYWGQGIRRADGTPVGMVGVI
                     LDVSDTVHANVALKAAKETAEEAGAAKSAFLANMSHEIRTPMNAIIGLSGLALKNEMP
                     PRIADYLNKIKQSGEHLLGIINDILDFSKIESGKLEMESVPFELEAVIDNVVNLISEK
                     VEDKGLELLCKVDANIPRTLVGDPLRIGQILINLANNAVKFTQQGNVRLAISIREMQG
                     DTVLLQFSVTDTGIGLAPEQVGKLFQSFAQADRSTTRKYGGTGLGLAISKSLVQAMGG
                     QIGVDSVVGAGSTFWFTASMGVGSSEGMLPRPGIDLHGRRVLVVDDSEAAALVLADTL
                     SELGFAVDQVNSSERALASLKQADSDERPYDFVLMDWQMPGMDGLETIQAIQQQHIRT
                     APLVLMVTAHRRLELIKGAEKLGIHHVLAKPVSGSMLVNTMMEIMGQGSRQVTHVSAK
                     VEPVEGQLGHLAGARILLVEDNEINQEVAIEMLSEAGFAVEVAENGQIAVNLVQARFA
                     EDLPYDIVLMDMQMPVMDGCTASRLIRESHGAAHFPIVAMTANAMKEDRDRCLAAGMN
                     AVVTKPIDPDDLWRALLKWVKVRDGMGVPVGASLRCRVLSMPGT"
     gene            10133..>10412
                     /locus_tag="PLB25_05335"
     CDS             10133..>10412
                     /locus_tag="PLB25_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOE41037.1"
                     /translation="MPGSVDAGNLMQELRTIQELNVDLGLTRASNKPDFYASMLRKFV
                     VAQEDATLRLRLALDRSDMVTVERIAHTLKGVAGNLGATVLPVMAEHLE"
BASE COUNT         2121 a         3003 c         3100 g         2188 t
ORIGIN      
        1 aaacggtggg ggcggagcca cgatttggga cgatatttat gtgcatgtct acattataca
       61 acatattacc aattgaatga aaaattaata catgtctaca tatttgcacc caaacaacac
      121 taacgtgttg atttcaaaag gaatccaacc aaatcaggat ggaacttcag tttaggcgct
      181 ggttgccacc tctggcgctg ccagtccggc ttgagctggc cgggccgcag ccggaccctg
      241 attcaggccc agtgcccccc gcaaactggc aaagagggtt gtcagttcag cgctggcggg
      301 cactatgagg ccttcaatgt cgtctgtgtt ccggccctcg cgcactgcct gctcgatctc
      361 cagcagcgtc tggcgcaagg gctcggcgcc catgctggcg cacaagccct tcaaagtgtg
      421 cgccaggcgt tgcgcggtgc ccgcatcacc atcggccaag gcctgacgga tattctcaag
      481 cgtgtgttcc tgggccttga cgaacttgcc cagcagggac acatacagct tgggattgcg
      541 gttgcactgg gtcaagcccc ggctggcgtc caggcccaca atctcatgca gggcgatcaa
      601 catctgctcc aacggcttgg tctgcggctg cggctgcggc tgaggtggcg cagcgctcac
      661 ggtgggcact tgggcaggac ctgccccgct acggggtttg acccaggcca gcagggcacg
      721 ccagagctcc tccgggtcta tgggtttgct gataaagccg ttcatgcccg cggccatgca
      781 gcggtctttg tcggctttca tggcattggc ggtcatggcc acaatcggca gcgcctccga
      841 ggtgaagctc tcacgaatca ggcgtgaggc ggtgacaccg tccatcaccg gcatctgcat
      901 gtccatcagc acgatatcgt acggctgggc atcggcaaaa cgggcatgca cctggtgaac
      961 gccgatctgc ccattgtcgg caacatcgac tgcaaagccg acgatttgca gcagttcgca
     1021 tgccaccatc tggttgattt catcgtcttc caccagcagg atgcgcgccc cggccatggg
     1081 tgccagcgcg gcctcggcag cgctggccgc ttggccctgc ggcgcattcg ctggcttgcg
     1141 cggtgcatgg ccttcaagtt gcatcatggt gtcgaccagc agggacgcgc ctaccggctt
     1201 ggccagcaca tgctcgaccc ccaaccgctg tgcgcccttg agcagttcct ggcgacggtg
     1261 ggccgtgacc atcaggacaa agggtgccga gcgggggttc atttcttgga tctgacgcac
     1321 cgcttccagc ccgtccatgc ctggcatttg ccagtccatc atgacaaagt caaaggccag
     1381 tccagcagct acggcctgcg ccacggcttg cagcgctgcg gcgccggagt tgacgctttg
     1441 caccacaaat cccagttcct gcagcaattc gcacagcacc agggcagcgg tatcgttgtc
     1501 atcgaccacc agcacccggc gcccgtgcag gtcaacgctg gggcgggtga tgattttctc
     1561 ggcagagcca atacccagcc gtgcggtgaa ccagaaggtg gagcctttgc cgtactcgct
     1621 ttgcacgccg acctcgcccc ccatgctctc agccaggctc ttgctgatgg ccagccccag
     1681 gccggtaccg cagtactggc gcgtggtgga gctgtcggcc tgctcaaagc ttttgaagag
     1741 ccggcccatt tgttcgggct tgaggccgat gccggtgtca ctgagggcaa agtgcaagag
     1801 cacctcggac gcggttgact ccagcacccg gatgctgatg cgcaactcac cgtgctcggt
     1861 gaacttgacg gcgttgttgg cgtagttgat caggatctgg ccgatgcgca aggggtcacc
     1921 gatgaggttc ctgggcacgg cattgtcaaa gctgcacagc agctccagat gtttggcctc
     1981 ggctttctgg ctgatcaggt tgaccacgtt gtcgatcacc gcttcgagct cgaagggcac
     2041 cgattcgatt tcgagcttgc ccgattcgat cttggagaag tccaggatgt cgttgatgat
     2101 gcccagcagg tgttcgccgc tttgcttgat cttgcttaaa tagtcctgga tgcgcggggg
     2161 catttcgttc ttcagggcca ggcccgacag gccgatgatg gcgttcatgg gggtgcggat
     2221 ttcgtggctc atgttggcca ggaagtcact cttggcaaca ttagcctgct ccgcttcaac
     2281 tttggcttgg atcaatgcga cttcagcgtt ttttcgggtc gtgacgtcgt tgaacagtac
     2341 caccacgaaa tccaattttt tatgccgtgt tcgaacggca cgtacagcca cttcaacggc
     2401 taggcgtttg ccggtgctat gtacaaagac ctgctcgcat tgatactcgt ccaactcgcc
     2461 tgccaatagg cgagcagact ttgcgaggac ggctgggcgc tcagatgcat caaccagatc
     2521 tacccatact tttttatgca attccgaagc ggaaaaaccc agcatttcgc acagagtctg
     2581 gttgatatcc agcaattcat ttgcagaccc tatggttgcc atgccgacca ttgaacgctc
     2641 aaaataggcc cgaaaacgtc gctcggctct ttctcgttcc tctcggaaaa cccggcgccg
     2701 ttgtgtttgc caagcaagca gggtcatcag caaggcgaca gccagaaaaa tcaacaactt
     2761 ccataacgtt ctgtttttcc agtccaccat gagtgagtcc gttttcactg agaccgcgac
     2821 gtccaaggtg gtatctccga cccgaccaaa caccaggaca cgcttcaccc catcggtagc
     2881 ggtaacaccc acataccggg tcatcttttc tgttgaagcc agatacttct tgaaggactc
     2941 ggcgcttcca atgttcttgc caacataagt actagggttt ggaagccggt agatgatgtc
     3001 tccctgccgg ttgtgaacag cggcagcatc tattgcccca ttgcgcagaa gctcgctcag
     3061 gacattgtcg aaaagctggg gagcggtaaa gatgacgacc accccctgaa gcacaccgtc
     3121 attgccacga attgccttcg acagggaaat aatgtaacgc cccccggtct ctttaaaggg
     3181 acgggacagc cagtttcgtc gggcttcctc gacacgggca tttcgatgga ctaaaaagta
     3241 ttcctgttga gctgcatcga gaccctcaag cctgggcagg acttctggca tacggatgct
     3301 tgcggcatcc acaacgcgcc ccatggaatc aagaacaaac aagacgtcta cttcggggaa
     3361 ctgttgcaac aggttcagct tgcgctggtg ggctgctgtg gcgggcaact tggggctgga
     3421 cagttcgtcc gtcaccacac tttgcgtgat catctccgta cgctgcagga ccgatgaaat
     3481 ctgcacatct gcgagtcgga catgtccctc gaaggcttga agttcccggt cgacagtggc
     3541 gtagtaatcg acgtagctat gcccgccaat ggatgcagcg acaagcacaa agaacatgct
     3601 cagcagtatc caggccttac ggcctttacc ttgcacactg tcagtcgagc cgcccattac
     3661 tggcagcccg tctcaccaca acagtcacat gaaatcgtgt ctgttccagc ctggcgttgt
     3721 tgttcgtcgt tgccatagct acgactatgc cgcatcctct cgcctagcca ggttgaaaaa
     3781 tttgtcgatt tcatttgata agctttttga tgaaacgaac gaccagttgc aacttcccac
     3841 aaagaatttg acgcggctgg tgatttcaga aagttcagtg ttgcgcctat cttttttcaa
     3901 ccgcaaagac aaatccacct cgccaatggc aatatccggc aaggctacgc tcaactcctt
     3961 gatcggccgg gccgccacgc ttctgagcgt ccagacaggc gttcggcctc gccgctccgg
     4021 taaccgaggt agccaaagtc cccaaaaacg caggaggtca ccgtcatcac gatgttggtg
     4081 aaccgcggtc gcgcggactg ccagagtcaa aatttcattt ctttacgcat tggaacttgc
     4141 tgacctacca tcgcgccact tgcaacagac cggggctgcc catccagacg aggtcgccag
     4201 aaaagccgct gtttgtctat ctttcgctgc gctcaatgct aggaaaaaac gccggttttg
     4261 tcagtcatcc aaatctgaaa gaacgtccct gtaagtccta gtctttgcta ggaatattcc
     4321 gaaattttga ggcactcatt ccaggtgact tctgtcgggg agcgggctga cttgccggat
     4381 ggactcaacg ctatgcgggc ggggtcgaat tggcgtccaa cgcggaagct ttttttggct
     4441 ggtctgtcaa gggggcgctt cgtgcagggc aacagcatgg aggtgcgggc cttgtcgcga
     4501 cacgtgtggc actttacggt tcaggttgaa accgcttact cgcgcggctt cccgctcgaa
     4561 ggctgggacc agtactgcaa gtgcttgagc atcctgctcc ttggcggtgc gctccatggc
     4621 ggcgcacaac tcgccgagct gcagtgcgcc aacggcccgt gccgacaatt tgagcttatg
     4681 cgcctgggcc tcgacttggc tggtctttcc tgccgcatag gcacccagta attccccggt
     4741 caacatggcg gcactgatgt agaagtctgc gacaaattga tccaccgtac cggcatcgtc
     4801 gccaatcaaa gcctccaaca cccttacatc cagcggcagg cagggtcggc gtcggccgcc
     4861 tgcgctgcga atgggatgac cgtctccggc tgctgcgaca agttggccaa gtcaattggt
     4921 gtggcaacca cctcaggctg ccagcggtcc agaatggcgc gcaattgtgt cagttgcacc
     4981 ggtttgctta ggtagtcgtc catgcccacg tcaatacagt gctcggcctc tcccttgagc
     5041 acattggcgg taaaggcaat gatgggcggg cggctcttgc cgacctcggc ctcgcggatg
     5101 gcggcgatca gctggtagcc gtccatcagc ggcatgtgca agtcggtgat cagtagacag
     5161 taggagccag actgccaacg cgccagcgct tcctggccat tgttgacaat gtcggccgtt
     5221 cggcccaata gcaccagctg ctgcagcatc actttctggt tgtattcatt gtcttcggcc
     5281 accaggatca ggctgccctg gcgacgggcg tcttcatggg acatgggcgt gtgcgccatg
     5341 ggtgccaggc tgggctggat ctgccggtcg ggcgccttgg cgcgcccggc agcaatcgcc
     5401 accgcttcaa gcagagcccg gccgggcagc gcagcagcca ttgcaaggcc accgattcgt
     5461 cactggcccg ctccgcgtat gctcgggcgt ggcgcaggtg gacgctcata tcgtcggcca
     5521 ggctgtcggt gcgaccgaaa accaggcagt tcaagccggc cacctgaaaa gtatcggcag
     5581 ccacgcccgg cgttgcaccg aggcgtgcaa acggcaaccg cacgctgaac accgaacccc
     5641 tccccaactc gctgccgacc gagatgtcgc caccatcgtg atggtgtttt tgccgctgat
     5701 ttttgggctt cgccgcacgc tgttttaccg ccgggtggtg ttttttgggg gttccttgct
     5761 gattgtgggg gtggcactgg tgtggctggt agagcgggtg tttgacctga cgctgattag
     5821 cgcctgacgc ggtggcctgg ggcgaggtcg caccaccagg caaaaccatt ctctgcctac
     5881 gttcaacaag tgatctgtgc gatgaaattt gcactttcac ccggccgcag gccataggct
     5941 attcgcagcc acccctgtag ctctgaaccg atttcagtgg gtgagcctat tcgcacagtg
     6001 tactggttca tcccgccaga tggcatcagc ttcacctttg gtcaggacga caacgccttg
     6061 ctgctagttc aagccactgg cacccaggag tttgcgcgtg acagcaagct ggcgttagct
     6121 cgcaaattga tagcccttat cgcagatagg ataaaggctg gaggccaaga ttgttaaaca
     6181 ttgaggtcaa gatcatcgac ccgcgcctgg ccgggcaatt gcccgcctac gccacaccag
     6241 acagcgccgg gctggacttg cgcgcctgtc tgagcgagcc gctgacgctg tcagccaacg
     6301 cctggcaact ggtgcgcacc ggcattgcca tttgcctgaa agacctgggt tctgccgcgc
     6361 agagcctgcc gcgctccggg ctgggccaca aacacggcag cgtgctgggc aacctggtcg
     6421 gcttgatcga cagcaactac caaggccaat tgatggtcag cgcctggaac cgcagcgaca
     6481 ccgcattcag atgcgcctga tcgcctttat cacccacagc gccgacatcc ggcaaatact
     6541 cgatcacatc ggggtggact cgccgccccg cacatagccc cggcacgcgg gccgccgttg
     6601 cgggctgacg gtggtgcgca gatcagagag gatgttgcgt ccgagccgga cagggatctg
     6661 gcagcgcaac ccgcgccaga cgacgaggtc gatagacaag tcaatgggag agtgagttaa
     6721 acggcgattg gctagacttg ccgtggggtg gcaccaagtc cggtgcacgc tgcgcaccgc
     6781 aaatcaccct tctgaacaca actcctggta gtgaatctgg cacgccgggc ggctaattgt
     6841 gggttttacg cggaacgagg gcgattccag ggtgcgacat ttgggctggc gcggttgaat
     6901 ttccgatccg cttgagccaa agcaatttct tgcgaatatg atgacgggcc gtgcattgca
     6961 aatgggcgta gacatggaaa atagtccacc gcagtgcaca cggcagcaca ttgatcaagt
     7021 aaatacgatt tgttggattc cgatgttttc atctccgacc cattgtctcc ttgtccccaa
     7081 aggggttcag tagcatgttg cagggcgcgc ggttgcgaac cattgttgcg ctgttgttgg
     7141 cactaggtat agcgggcctg gtgctgtggg ccagagacca gttggtatcg gcacagcgtg
     7201 tagagcgcct gcaactggaa accctggcca tggcgtcgtt ggtcgaatcc gtcagcaaca
     7261 agggtgtggt catgagcggc gcttcgctga tcggggtctt tgatgtccga gccaagcgca
     7321 tgttgtccag cgccgcggtt gccatggatt tggaggcttt ggcagacttc gccaccgtca
     7381 ttggattgct gcatgtccag ggcgcatcaa ttctgaacgc cattggcgtt acccaggctt
     7441 cgttgaatgc ggctggaacg tttggcgggg caggtgccaa ctttttgcaa aagccctata
     7501 tacgaaaagc tttgtccggc gtgtctgcgg tcaacctcga agtggatgcc actaccgcag
     7561 agcgtatgct gcacttcgct gccccggttt ttgaagagcg ctccaccagc tccaaggtgc
     7621 tgggggtcta ccacctggag accaatgtcg cggaggtcga caaagtgctg gccaagtatc
     7681 tgccgcgcac cgcgctgctg gtgtctcccg aaggcacggt tttcgccagc aatcgaagcg
     7741 tgcaggattt gatgccatta cttgcacagt ccggtgaaac ggctgaaaag aacgcgagat
     7801 ttggttacct ggcgctgcgc gcacaggtcc cgtctggaac gaacggtttg acacggtttg
     7861 aggtgaaaga cttccatgtc accaacgtca aactgggctg gccggcagaa cagggggcat
     7921 ggcagactgt gctgatggag cccagcaatg actccgctga aatgtggtcg cgtctgatct
     7981 tgggcttgtc ggtgtttagc gtgctgtttg gcagctacag catgctggcg cgccacaatg
     8041 ccgcgcaggc caggttccgg caggtgctgc aagccaagca ggtagagcag gaagcgtccc
     8101 tgcgtgcata cgcggaccag gtggattttc aggcgtgcct gatggatgcg atcccgacgc
     8161 ccttgtttgt gaaggatgcg caaggtcgca ctgccggcat gaaccgagcc tatgaagaag
     8221 cctacggtgt gcggcgccaa gactacgtgg gcaaaacgct gttgcaaacc agtttgcgca
     8281 acctcgatgc ggcacagcgt gcgctctatt ttgaggagca gattgacatc attgcctcgg
     8341 gtgcactggt tcacaagaaa cgcgagatgt gctgggccga tggtgccatc caccatgagt
     8401 tgtactgggg ccagggcatt cgcagagccg acggcacacc agtaggcatg gtgggcgtga
     8461 tactggacgt gtcggacacg gtgcacgcca atgtcgccct gaaagctgcc aaggagacag
     8521 ccgaggaagc gggtgccgcg aaatcggcct tcttggcgaa catgagccac gagattcgca
     8581 cgcccatgaa cgccatcatt ggcctgtcgg gcctggccct gaagaacgag atgccgccgc
     8641 gcattgccga ctacctcaac aagatcaagc aaagcgggga gcacctgctg ggcatcatca
     8701 acgacattct ggacttctcc aagatcgaat cgggcaagtt ggaaatggag tccgtcccct
     8761 ttgaactgga agccgtcatc gacaacgtgg tgaacctgat ttctgaaaag gtcgaagaca
     8821 aagggctgga actgctgtgc aaggttgatg ccaacatccc caggactttg gtgggcgacc
     8881 cgttgcgcat cgggcagatc ctgatcaacc tggccaacaa cgcggtgaag ttcacgcagc
     8941 aaggcaatgt gcggcttgcc atttccattc gggagatgca gggtgacact gtgctgctgc
     9001 aattcagcgt aactgatacg gggatcggct tggcgccaga acaggtcggc aagttgtttc
     9061 agagctttgc tcaggcggat cgctccacca cccgcaaata tggtggcacc gggctgggcc
     9121 tggccatcag caaaagcctg gtgcaagcca tgggcggcca gattggtgtg gacagcgtgg
     9181 tgggcgcggg ctccaccttc tggtttaccg ccagcatggg ggtgggttcc agcgaaggca
     9241 tgctgccccg gcccggcatt gatttgcatg gtcggcgcgt gctggtggtg gacgacagcg
     9301 aagccgccgc gctggtgctg gctgacacgc tctctgaatt ggggtttgcc gtagaccagg
     9361 tgaactccag tgagcgggcg ttggcaagcc tcaagcaggc cgactccgac gagcggcctt
     9421 acgacttcgt cctgatggac tggcagatgc cagggatgga tggcctggag accattcagg
     9481 ccatccagca acaacacatc cggactgcgc ctttggtgtt gatggtgacc gcgcaccgcc
     9541 gtctggaact gatcaaaggc gccgagaaac tcggaataca ccatgtgctg gcaaaaccag
     9601 tcagcggctc catgctggtc aacaccatga tggagatcat ggggcagggt tcgcgccagg
     9661 tcacccatgt cagcgccaag gtggaaccgg tcgaaggcca gctcggacac ctggccggtg
     9721 ctcgcatctt gctggtggaa gacaacgaaa tcaaccagga ggtggccatc gaaatgctga
     9781 gcgaagctgg ctttgctgtg gaggtggccg aaaacggaca gatcgccgtg aacttggtgc
     9841 aggcccgttt tgcagaagac ctgccctatg acattgtgct gatggatatg caaatgccgg
     9901 tgatggacgg ctgcaccgca tcccgattga tacgcgagag ccatggtgca gcgcatttcc
     9961 cgatcgtggc catgaccgcc aacgccatga aggaggatcg cgatcgttgc ctggcagccg
    10021 gcatgaacgc cgtggtgacc aagcccatcg acccagatga tctgtggcgc gccttgctca
    10081 aatgggtcaa ggtgcgcgat ggcatgggtg tacccgtcgg tgcaagcctg cggtgccggg
    10141 ttctgtcgat gccgggaacc tgatgcagga attgcgcacc atccaggagc tgaacgtgga
    10201 cctgggcctg acccgagcct ccaacaagcc cgatttctat gcctccatgc tgcgcaaatt
    10261 tgtcgtggca caggaagacg ccactctgcg attgcgcctg gcgctggacc ggtcggacat
    10321 ggtcacggtt gaacgcattg cgcataccct caagggcgtg gccggcaatc tgggggccac
    10381 cgtattgccg gtgatggccg agcacctgga aa
//
LOCUS       DAOSLL010000083        10388 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6278_length_10388_cov_4.650731, whole genome shotgun sequence.
ACCESSION   DAOSLL010000083 DAOSLL010000000
VERSION     DAOSLL010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10388)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10388)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10388
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6278_length_10388_cov_4.650731"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..159
                     /locus_tag="PLB25_05340"
     CDS             <1..159
                     /locus_tag="PLB25_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017406912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
                     /protein_id="HOE41038.1"
                     /translation="GHGQGVFRQAHDVIVRAIETVLRHGPRTPDLGGEASTTTMGEAL
                     AAHIALCE"
     gene            456..1700
                     /locus_tag="PLB25_05345"
     CDS             456..1700
                     /locus_tag="PLB25_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HOE41039.1"
                     /translation="MKIKSAISRCSQWMRPFGLLACVWYLSGCGGGGSGGDSPPVVVP
                     SPPPIVVPESPVINVNGPGHFTDAVLLKTLSAAEVASEIELAGKSEPYLKPKYAVKAY
                     RLTYLTLDGYGQEILASALVVVPQKPVGAPSPLLGYQHATIETDLEAPSSLSEPASPE
                     VIIASMGYIVQAADYVGYGASKGAEHPYLLSAPSASAVIDLLTAAKYWRQTQGIVDNR
                     QLFLTGYSEGGYVTMAAHRALQAGNSTHRQEIVSVMPAAGPFNVLLTLDEAMKLARQQ
                     YPVLGFFLSPGFLHLLSDSDRDNVRDFVLKLLLGGESDVTFMPTVLGNYFADNRSAIE
                     TQSNVDVWRPEVPVHLFHGRDDTTVSYLNSSHTLRVMQDQGAGALVSLTDCNVKPAGH
                     LECVPPYFEFMLSTFGKVAKDL"
     gene            1980..2483
                     /locus_tag="PLB25_05350"
     CDS             1980..2483
                     /locus_tag="PLB25_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41040.1"
                     /translation="MQLPSIDRIQSLRPLAAESASLATGRVVPVAPVNPPALDSPAAA
                     PSPSVINLVNQAEKPSAGEGVYSSVSDPTRKGADAATGTKDWTIHRPVVEKAEDPPPE
                     PLSKILLDHLKSLWTASASAVQVQQQVKSQAELVLPGVKTTPGAIATEVFTYSPTKIN
                     KADKTQT"
     gene            complement(2537..4333)
                     /locus_tag="PLB25_05355"
     CDS             complement(2537..4333)
                     /locus_tag="PLB25_05355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HOE41041.1"
                     /translation="MMLSQKFAAFKTFTAQAWALTKPYFASEQKWKARVLLASIVALN
                     LGLVYMAVLFNDWNKLFYDALQEKNAAVFWSQLGRFTWLAFGFIVIAVYKFYLTQLLE
                     MRWRAWMTGQYLDRWLSNQAFYKLELMRFVTPRDDPNAVPQTARNPDNPDQRIQEDIN
                     SFTSYTISLSMGLLNAVVTLASFVGILWGLSGSFAFTLGGTGFNIPGFMVWMAVLYCL
                     AGSVLTHYIGRPQIALNFQQQRLEADFRHHMVRVREYSESIALDRGESVEQSQLDTRF
                     GRVLGNYLALIKAQKSLIWFTSFFGQAAIVFPFVVAAPRFFSGAIQLGELMQISSAFG
                     QVQGSLSWFVDNYSSLAAWRATTDRLTSFEASFQALALTDKALVAIDSIALETPDLDL
                     ELPNGVVLLKQAHLSARAGDSILLQGPSGAGKSTLFRALAGIWPFVSGHLQRPANSMF
                     IPQRPYFPNGPLRDALAYPQSAALYTDAELQEALTSALLPNLMTRLDEVGAWSQTLSG
                     GEQQRLAIARVLPKKPAWIFADEATSALDAKTEESIYKTLLAHVQSVQGAIVSIAHKP
                     SVAAFHSRRWVLEKAPQGNPASYRIQAAEGVN"
     gene            4739..6205
                     /locus_tag="PLB25_05360"
     CDS             4739..6205
                     /locus_tag="PLB25_05360"
                     /inference="COORDINATES: protein motif:HMM:NF014427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HOE41042.1"
                     /translation="MKIIITRIFLLILLAIVAGCGGGGSDSTPAPPTTPTLSSISVTP
                     ADATLDFGLSQQFAAIGTYSDGSTKDLTSSAKWTVASTTILTISTAGLATTKAVGTET
                     VTATVGTVAGSTKLTVKAAFTALVPGGFHTLALKSDGSLFAWGRNLQGQLGDGTTIDS
                     AQPIQVGTVKTWTKVAAGEFHSVAFRADGTLWAWGFNQSGQLGDGTAVNRNAPTKIGT
                     ATTWVALAAGKSHTMAIKKDGTLWAWGNNTSGQLGLGTVTSSSVPIQIGTATNWTSVS
                     AGDGHTVARRADGTIWGWGDNTRGQLGLMPLTMVPYTKQVSVPTQIGPDPSSRWISVS
                     AGGEHSLAIRSDGKLFAWGSNGNGQLGIEVKPGGQTAEDLLGGRGTPTQVGEDFRWAI
                     ALAGRMHNHATKTDGTLWAWGSNSQAQLGDGTMLDKTTPIQIGTATNWLSIAPGSEHV
                     LGLTSDGSAWAWGRNMEGQQGNGFVTTYPVLIPTLLPY"
     gene            complement(6262..7227)
                     /locus_tag="PLB25_05365"
     CDS             complement(6262..7227)
                     /locus_tag="PLB25_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HOE41043.1"
                     /translation="MSLTLDGKLVVAISSRALFDFEEENQVFEQGDDRAYMALQLQRL
                     DTPARPGVAFSLVQKLLAFNQPTPNAVVLPQPTASTVDGKPSQPVEVVILSRNDPVSG
                     MRVFRSAQHYGLAIERGSFTRGQAPWRYLKPLHANLFLSAHLSDVRAALAAGVPAAQV
                     YPLSAHASDAHPFEVRIAFDGDAVLFSDEAERVYQAQGLQAFQQHEIEKAALPLPDGP
                     FKPLLVALQRLQQAGTPQMRIRTALVTARSAPAHERAIRTLMNWNIEVDEAMFLGGLS
                     KGEFLREFEPDFFFDDQTGHIESAAQHVPSGHVASGVRNQGVKQR"
     gene            complement(7224..8786)
                     /gene="ilvA"
                     /locus_tag="PLB25_05370"
     CDS             complement(7224..8786)
                     /gene="ilvA"
                     /locus_tag="PLB25_05370"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HOE41044.1"
                     /translation="MKNPDLTSFDYLKKILTARVYDVAIETALEPALNLSARLNNTVL
                     LKREDQQPVHSFKLRGAYNKMAHMNPAQLKSGVICASAGNHAQGVALSAQRLGARAVI
                     VMPTTTPQVKVDAVRGFGGEVVLFGDSYSDAYSHAVDLEKEQGLTFVHPFDDPDVIAG
                     QGTIAMELLRQHQGRLDAVFVAIGGGGLISGVANYIKAVRPEIKVIGVQMNDSDAMVQ
                     SVSAQHRVTLTDVGLFSDGTAVKLVGKETFRITSELVDDYLLVDTDAVCAAIKDVFVD
                     TRSIVEPAGALAVAAIKQYVAQHKTQNETYAAILCGANMNFDRLRFVAERAEVGEERE
                     ALLAVTIPEERGSFRRFCELIGDLPSFGGAKTLRNVTEFNYRISDANKAHVFVGLTTA
                     ARGESGKIAAYFGQHHFEALDLTHNELAKEHIRHMVGGHSMLAKDERLMRFVFPERPG
                     ALLKFLSMMRPNWNISLFHYRNQGADYGRILVGLQVPLADNAAFTDFLQTLGYPCVEE
                     TANPVYQMFLQS"
     gene            8867..9316
                     /locus_tag="PLB25_05375"
     CDS             8867..9316
                     /locus_tag="PLB25_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HOE41045.1"
                     /translation="MECTVSWTGALGTRSGMGFVAETGSGHAMVMDGAPDAVKPENGG
                     LNLAPRPLELLLAGTGGCTAYDVVLILKRGRHDVRGCTVKLSAERADTDPKVFTHINL
                     HFTVTGRGVPVLAVERAIAMSHDKYCSASVMLGKTAQIATSFEVQEV"
     gene            complement(10182..>10388)
                     /locus_tag="PLB25_05380"
     CDS             complement(10182..>10388)
                     /locus_tag="PLB25_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HOE41046.1"
                     /translation="NNGYSMPFLMQAETFAQQAFKAIETQVSYRVIPWQMGVVAKLLR
                     LLPNWLFDKALAGRPRKPRAQGSD"
BASE COUNT         2161 a         2993 c         3033 g         2201 t
ORIGIN      
        1 ggtcatggac aaggtgtctt tcgccaggca catgatgtga tcgtgcgggc gatcgagacg
       61 gtgctcaggc atggcccgcg cacgcctgat ctgggcggag aggcttcaac gaccacaatg
      121 ggcgaagcac tggcagcgca tatcgcccta tgcgagtgac gggtgacgca tggcaaccag
      181 gcccgtgatg cccgggagcc tgttggcctg atcgaacacc ggctgcgcac tgaccacagc
      241 gcaacgtctt gcaccggttg attgacccaa gctgggctgt gggcctgcga cgctggcaca
      301 agctggccgc gcaggggtcg attcccgtga tcaacgccat cggtccggca ggctcccggt
      361 tgccgtggtg cgagctgaaa tgcttatttt tcagcgtata ttggcaatgg tggaaaatcg
      421 gcttgaacat atttttgcca gaaatggggg cttgtatgaa gatcaaatcg gctatttctc
      481 gttgcagtca gtggatgcgc ccgttcggcc tgctggcttg cgtgtggtat ttgagcggct
      541 gcgggggcgg tggctcgggt ggggactctc cgccggttgt ggtgcccagt ccaccgccca
      601 ttgtggtgcc cgagtcacct gtcatcaatg tcaacgggcc gggccatttc actgatgccg
      661 tactgctgaa gaccctttcg gccgctgaag tagcgtctga gattgagctt gctggcaaat
      721 ccgagccgta tttgaaacca aaatatgcgg tcaaagccta tcgactgacc tatctgacgc
      781 tcgatgggta tggtcaggaa atactcgctt cggcgctggt ggtcgtgcca caaaagcctg
      841 tcggcgcgcc aagtccgctg ctcggctacc agcatgcaac cattgaaact gacctggaag
      901 cgccgagcag tctgtccgaa ccggccagcc ccgaggtgat catcgcttcg atgggctata
      961 tcgtccaggc agccgattac gtcggctacg gcgcaagcaa gggggctgaa catccctatt
     1021 tgctgtcggc accttctgca tccgccgtga tcgacctgct gacggcggcc aagtactggc
     1081 gccagacgca gggcatcgtc gacaaccggc aactgttttt gacgggttac tcggaaggtg
     1141 gttatgtcac catggcagcg caccgggcct tgcaggccgg caattcgacg caccgtcagg
     1201 aaattgtcag cgtgatgccc gccgccggcc ccttcaacgt gctcctgacg ctcgatgaag
     1261 caatgaaact ggctcgccag caataccctg tgctcggttt ctttctgagt cctggtttcc
     1321 tgcatttgct cagtgattcg gaccgtgaca atgtgcgcga tttcgtcctc aagctgctgc
     1381 tgggcgggga atcggatgtg actttcatgc ccaccgtgct tggcaattat tttgccgaca
     1441 accggtccgc cattgaaacc caaagcaatg tcgatgtctg gcgcccggag gtgccggtgc
     1501 atctttttca tggtcgggac gacaccacgg tttcgtacct gaattcaagc cacaccttgc
     1561 gggtgatgca ggaccagggt gccggcgcac tggtcagcct gaccgactgc aatgtgaagc
     1621 cagccgggca tctggagtgc gtgccacctt actttgagtt catgctgagc acctttggca
     1681 aagttgcgaa agacctttga acactcggtg agccgcatga gcatcctttt ttgggggaac
     1741 gttgtcgtgc caaaggactc gctttgctgg cgggcaatgt tgccgatggg cgttcaccga
     1801 agcacatatg cgctggacgt tgaggcccgt ttgcctgact gatttcagcg gctgcactgc
     1861 ccgatgacat caaaaacgcc agttttttta aaaatgggcc ttaattaccc atcaattctg
     1921 ccgttaatga gtctacgaac ggaggaaaga cccagaccca ttctgccgcc tgatacacca
     1981 tgcaactacc atccattgat cgaatccaga gcttgcggcc actcgccgca gagtcggctt
     2041 cgcttgcgac tggtagagtg gttccggtag caccagttaa tccgcctgcg cttgacagcc
     2101 ctgccgcagc gccttcgcct agtgtgatca acttggtcaa ccaggctgaa aagcccagcg
     2161 ccggtgaagg tgtttacagc agtgtttctg accccacccg caaaggtgca gatgccgcca
     2221 ccggcaccaa agactggacc atccaccgcc cggtggttga aaaggccgaa gatccgccgc
     2281 cggagccact gtccaaaata ctgcttgacc atctcaagtc actttggacc gccagcgcca
     2341 gcgcggtgca ggtgcagcag caggtcaaaa gtcaggctga gctggtgttg cctggtgtca
     2401 agactacacc tggtgcgatt gccaccgagg tttttaccta ctcaccgacc aaaatcaaca
     2461 aggctgacaa aactcagact tgagccagag gtgggtcagc accagcctga agcccttgct
     2521 caggtgctgg tgtttttcag ttgacaccct cggcagcctg aatccggtag ctggctgggt
     2581 tcccctgggg ggctttttcc agcacccatc gtcggctatg aaaagctgcc acgctgggtt
     2641 tatgagctat tgaaacaata gcgccttgca cagactgcac gtgggctaga agtgttttat
     2701 agatggattc ttcggtcttg gcatcaagcg cgctggtggc ctcatcggca aatatccagg
     2761 ctggcttttt cggcagcacc cgggcaatcg ccaggcgttg ttgctcacca cccgagagtg
     2821 tctggctcca ggccccgacc tcgtcaagcc gggtcatcag attgggcagc agggcgctgg
     2881 tgagcgcctc ttgcagttcg gcatcggtgt acagtgccgc gctttgcggg tacgccagcg
     2941 cgtcgcgcag tggcccgttc ggaaagtatg gtcgttgcgg gatgaacatg ctgttggcgg
     3001 ggcgctgcag gtggccactg acaaatggcc agataccagc cagcgcccga aacagcgtgg
     3061 atttgcccgc acctgacgga ccttgcagca agatgctgtc tccagctcgt gcgctcaggt
     3121 gagcctgctt cagcagcacc accccgttgg gcaattccag atccaggtcg ggagtttcaa
     3181 gagctattga atcaatagct accagcgctt tatcagtaag ggctagagcc tgaaaagatg
     3241 cttcaaaact ggtcaatcgg tcggttgtgg cgcgccaggc agccaggctg ctgtagttgt
     3301 ctacaaacca gctcaacgag ccctgcacct ggccaaaagc gctggagatc tgcatcaact
     3361 cacccaactg gatggccccg ctgaaaaagc gtggtgcggc caccacaaac ggaaacacaa
     3421 tcgctgcctg gccaaaaaaa ctggtgaacc agatcaggct tttttgcgcc ttgatcagcg
     3481 ccaggtagtt gcccagcacc cggccaaaac gggtatcaag ctggctctgc tcgaccgatt
     3541 ctccgcggtc cagcgcaatc gactcgctgt actcacgcac ccgcaccatg tgatggcgaa
     3601 agtcggcctc gaggcgctgc tgctgaaaat tcagcgcaat ttgcggtcgg cctatgtagt
     3661 gcgtcagcac actgcccgcc aggcaataca gcaccgccat ccagaccatg aagcccggaa
     3721 tgttgaaccc ggtgccgccc agcgtgaagg caaaactgcc cgacaagccc cacaaaatgc
     3781 caacaaagct ggccagcgtt accacggcgt tgagcaaacc cattgacagc gagatggtgt
     3841 aactggtgaa actgttgatg tcttcctgaa tccgctggtc tgggttgtcc gggttcctgg
     3901 cagtttgcgg caccgcattg gggtcgtcgc ggggggtgac aaagcgcatc agctccagtt
     3961 tgtagaacgc ctggtttgac agccagcggt ccagatattg acccgtcatc caggcgcgcc
     4021 agcgcatttc aagcagttgc gtcaggtaaa acttgtatac cgcaatgaca atgaaaccaa
     4081 atgccagcca ggtaaagcgc ccgagttgcg accagaacac ggcagcattt ttttcttgca
     4141 acgcgtcgta aaacagcttg ttccagtcat tgaacagcac cgccatatag accaacccca
     4201 ggttgagcgc cacaatcgat gccagcagta ccctggcttt ccatttttgc tctgaagcaa
     4261 agtagggctt ggtgagcgcc caagcctgcg ctgtgaaggt tttgaaggca gcgaattttt
     4321 gggacagcat catggtctgg cagaacaagt ggacggtttg acgttagttt ggaccgcgat
     4381 gctaactgca tatgagcttg ttgcatctgc accgcagcag ggtcgcagtc cctgcgcgtg
     4441 ccggaccaat ggcccaccgg ctgacaaccg accgcagcgg cccaattcga atcaactcct
     4501 ggatctgcca atgggctagg ggctggcaaa tccggcccgc accagccgcc cggcggacga
     4561 ttcgcgtgac caatgcaagt catcagaccg gctccttgca ggtgaagtcc cggaacaggc
     4621 aattgccgcg acaagtcggg cctgcttgtg ctttacaaca gatgttcaaa gactagaatc
     4681 gatttcaggc tgcacccatg aagcgacttg cctgtgaccc ctagcaaact gaaataccat
     4741 gaaaatcatc attaccagaa tttttctcct gatcttgctg gctatcgtgg ccggctgtgg
     4801 tggcggtggc agcgacagca caccggcacc tcccacgaca ccgactttgt cttcgatcag
     4861 tgtcacgccg gctgatgcca ccttggactt tggtctgagc cagcaattcg ccgcgattgg
     4921 tacctactct gacggatcaa cgaaggacct gacaagctct gccaagtgga cggttgccag
     4981 caccaccatt ttgacgatca gcacggccgg cctggccacc accaaggccg tgggcaccga
     5041 aaccgtgact gccaccgtag gcaccgttgc gggcagcacc aagctgacgg tcaaggcggc
     5101 gttcacagcc ttggtgcctg gtggttttca cacccttgcg ttgaagtccg acggcagttt
     5161 gttcgcctgg ggcaggaact tgcagggaca gttgggtgac ggcaccacga tcgatagcgc
     5221 gcaacccatt caagtcggta ccgtcaagac atggaccaag gttgctgcgg gagaattcca
     5281 ttcagtggca tttcgggctg acggcacact gtgggcgtgg ggctttaacc aatctggtca
     5341 actgggcgat ggcactgctg tcaatagaaa tgcgccgacc aaaatcggta ccgctaccac
     5401 ctgggtcgcg ctggcggcgg gcaagtcgca cacgatggcc atcaaaaaag atgggacgct
     5461 gtgggcctgg ggtaataaca cctctggcca actgggtttg ggcacggtga cctccagcag
     5521 cgttccgatt caaatcggca ctgccaccaa ttggacatcg gtttctgcgg gtgacggtca
     5581 tactgttgcg cggcgtgcgg atggcacgat ttggggttgg ggcgacaaca ccaggggcca
     5641 gttgggcttg atgcccttga caatggtgcc gtatacaaag caagtcagtg ttcctacgca
     5701 aattgggcct gatccatcaa gtcgatggat ttcggtgtca gcgggagggg agcactcgct
     5761 ggcgattcgc tctgatggaa agcttttcgc ctggggttcc aatggcaacg gccagttggg
     5821 cattgaggtc aagccaggag gccagactgc tgaagatttg ttgggtggca gaggtacccc
     5881 gacccaggtc ggggaagact tccgctgggc cattgcgctg gcgggtcgca tgcacaacca
     5941 tgccaccaag accgacggca ccctgtgggc ctggggctcg aacagccagg cgcagctcgg
     6001 cgacggcact atgcttgaca aaaccacgcc gatacagatt ggcacggcca ccaattggct
     6061 gtcgattgcg ccgggcagcg agcacgtgct tgggcttaca tcggatggct cagcctgggc
     6121 ctgggggcgc aacatggaag gtcagcaagg caatggtttt gtcacaacgt acccggtgct
     6181 gattccaacc cttctgcctt actgaacaag cgacttgcaa cgacgctgtg cgcccaaatc
     6241 cgccggtgca aatcggggaa gttatctttg cttgacccct tgatttcgca cgccgctcgc
     6301 aacatgaccg gatggcacgt gttgtgcggc cgactcgatg tggccggtct ggtcgtcaaa
     6361 gaagaaatca ggctcaaatt cacgcaagaa ttcgcctttg gacaggccgc ccaaaaacat
     6421 tgcttcatcg acttcgatgt tccagttcat cagggtgcga atcgcccgct catgcgcggg
     6481 tgcgctgcgc gcagtaacca gcgcagtgcg gatgcgcatt tgaggtgtac cagcttgctg
     6541 cagccgttgc agcgccacca gtaaaggttt gaaagggccg tcaggcagtg gcaacgcggc
     6601 tttttcaatc tcgtgctgct gaaacgcctg cagcccctgt gcctggtata cccgttcagc
     6661 ctcgtcgctg aacagcaccg cgtcgccgtc aaacgcgatc cgcacttcga aagggtgagc
     6721 atcgctggcg tgcgctgaca gcgggtacac ctgcgcggct ggtaccccgg ccgccagggc
     6781 agcgcgcaca tcgctcaaat gggcgctcag aaacagattg gcgtgcaacg gcttcaggta
     6841 gcgccagggt gcctgacccc gcgtgaaact gccgcgctca atggccagtc cgtaatgctg
     6901 cgccgagcga aacacccgca tgcctgacac cgggtcgttg cgcgacagga tcacgacctc
     6961 tactggctga ctgggtttgc catcaactgt gctggcggtt ggctggggca gcaccaccgc
     7021 atttggggta ggctggttga acgccaacag cttttgaacc agcgaaaacg ccacgcctgg
     7081 cctggccggt gtgtcgagcc gctgcaattg cagcgccatg taggcgcggt cgtcgccttg
     7141 ctcgaaaacc tggttttctt cctcgaagtc aaacagtgcg cggctcgata tcgccaccac
     7201 cagcttgccg tccagcgtta ggctcatgac tgcaaaaaca tctggtacac cgggttggcg
     7261 gtctcttcca cgcacgggta gcccagggtt tgcagaaagt cggtgaatgc cgcgttgtct
     7321 gccaagggca cctgcagccc gaccaatatg cggccatagt cggcaccctg gttacggtaa
     7381 tggaacaaac tgatgttcca gtttgggcgc atcatgctca ggaatttcaa tagcgcacca
     7441 gggcgctcgg ggaacacaaa gcgcatcaga cgctcgtctt ttgccagcat cgaatgcccg
     7501 cccaccatgt ggcgaatgtg ttccttggcc agttcgttgt gggtcaggtc gagtgcctca
     7561 aagtggtgct gtccgaagta cgcggcaatt ttgccggact cgccccgggc ggcggtggtc
     7621 aggccgacaa acacatgtgc cttgtttgcg tcgctgatgc ggtaattgaa ctctgtcacg
     7681 ttgcgcaaag tcttggcacc gccaaagctc ggcaagtcgc caatcagctc gcaaaagcgc
     7741 ctgaagctgc cacgttcttc aggaatcgtc acggccagca gggcctcgcg ttcttcgccc
     7801 acctcggccc gctcggcgac aaagcgcagg cggtcaaagt tcatgttggc accacacaag
     7861 atggccgcat aggtttcatt ttgagtcttg tgctgggcca cgtattgttt gatggcagcc
     7921 accgccagcg cgccggccgg ctcgacgatg ctgcgggtgt caacaaacac gtccttgatg
     7981 gcagcacaca ccgcgtcagt gtctaccagc agatagtcat ccaccagttc gctggtgatg
     8041 cggaatgttt ctttaccgac caatttgact gcagtgccgt cagaaaacag accgacatca
     8101 gtcaatgtca cgcgatgctg tgcgctaacc gactggacca tggcatccga gtcattcatc
     8161 tgcacgccaa tcaccttgat ctcgggacgc acggccttga tgtaattggc cacacccgaa
     8221 atcaggccac ccccaccaat cgccacaaac accgcgtcaa gtcggccttg atgctggcgc
     8281 agcagttcca tggcaatcgt gccttgaccg gcgatcacgt cagggtcgtc aaacgggtga
     8341 acaaaagtca gcccttgctc tttttcaagg tccacggcat ggctgtaagc atccgagtag
     8401 ctgtcgccaa acaggaccac ctcgccgcca aagccgcgca ccgcatcgac cttgacctgc
     8461 ggcgtggtgg tcggcatgac gatcacggcg cgcgcgccca gccgctgcgc gctcagtgcc
     8521 acgccttgcg cgtggttgcc agcggaggcg caaatcacgc cgcttttcag ttgcgccggg
     8581 ttcatgtggg ccatcttgtt ataggcgccg cgcagcttga agctgtgcac cggttgttgg
     8641 tcttcgcgct tgagcaggac ggtgttgttc aaccgggcac tcaagttcag ggctggttcc
     8701 agcgcggttt ctatcgccac gtcataaacg cgggccgtca ggattttttt caggtagtca
     8761 aatgaggtca gatcaggatt tttcataggt ggctgatgat aagtgggcag acaaagcggg
     8821 gaaaacgtga caattgcgct ctttgacttg ttgaaggatc atgcaaatgg aatgcacagt
     8881 cagttggacc ggcgctctgg gtacgcgctc tggcatgggg tttgtggccg agactggcag
     8941 tggtcacgcg atggtgatgg atggcgcacc cgatgccgtc aagcccgaaa atggcggact
     9001 gaacctggca ccgcgcccac ttgagttgct gctggctggc accggcggtt gcaccgctta
     9061 tgacgtggtg ctgatcctca agcgcggtcg ccacgatgtg cgcggctgca ctgtcaagct
     9121 cagcgccgag cgggcggaca ccgacccgaa ggtgtttacc cacatcaacc tgcatttcac
     9181 cgtgactggc aggggcgtac cggtgctggc agtagagcgc gccattgcca tgagccacga
     9241 caaatactgt tcggccagcg tcatgctggg caagacggcg caaatcgcca ccagttttga
     9301 ggtgcaagag gtatgaggca aagcatcagg ccatacgcag gcggacaccg ccttggcagg
     9361 cttggtcaat caggcaaatc aggttttgac gctctgaggt tgaggtcaag ttccttgccc
     9421 tcccactagg ccggtagcca gttttgccgc accaactgtg ctgcggcttc tggccgtgca
     9481 tgtctttgcg acgcagatca catgccaatt ggtgttgctt gagtcagggt tgacgcagcg
     9541 ctgcatctgc cggtgtgaca ccaggccgat caaattcgca caaatcgcgc cgctgttggc
     9601 gcagttggtg attgaacagg cccatttcac ggcatcagat gtgcagatca tggcttctat
     9661 tcatcgctgt caaacgctgt acgacacccc aagtggccgt tgagcgcttg gctggcaaaa
     9721 cccgagcctg attgatgtcc gattcctcga gtcaaggtgt catgcgaaag atggcgttcg
     9781 tctgcgatgt attggagctt cttggctcct cgctcattca cgtgcattgc tcaatgcctg
     9841 ttctttgggt ttcgcggggt aagttttcgt ttcaaaaggt atcgcattga tgcggatcat
     9901 ctgcagttct tgcaggcgac aactactttg actcagaggg ttccatatcc gaaacgccat
     9961 gtcgattgaa ctgccgattc aatctcagga acatcatgcc ccgccttgtc cgcatcgccg
    10021 ttacagggca agcccactga ggtgtggatt gggctggctg aagatcagcg ccaatcagtt
    10081 caacagttgc acaaagatct tggcgatgca caatctttgt tcggaggaaa tccaggacga
    10141 taaccatcat ccgtgcacac cagtgtttca gtggtggcgc ttcaatctga cccctgtgcg
    10201 cgtggcttgc gggggcgccc ggccagcgcc ttgtcaaaca gccagttggg caacagacgc
    10261 agcagcttgg ccactacgcc catctgccag ggaatcacgc ggtagctgac ttgtgtctcg
    10321 atggccttga aagcctgttg ggcaaacgtc tcagcttgca tcaaaaacgg catgctgtag
    10381 ccattgtt
//
LOCUS       DAOSLL010000084        10371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6293_length_10371_cov_5.351396, whole genome shotgun sequence.
ACCESSION   DAOSLL010000084 DAOSLL010000000
VERSION     DAOSLL010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10371
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6293_length_10371_cov_5.351396"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="PLB25_05385"
     CDS             complement(<1..869)
                     /locus_tag="PLB25_05385"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE41047.1"
                     /translation="MSNKIHTPVTARRVAFLGLGVMGYPMAGHLAEAGHSVTVYNRTA
                     SKSIAWCADYTRATVRNDAIYQAQTPRQAATGAALVFCCVGNDDDLRSVTLGADGAFA
                     GMSAGAVLVDHTTASANVARELYAQAKSQGLGFVDAPVSGGQAGAQNGTLTVMCGGDQ
                     ADFETAEPVARAYSKAFTRLGESGAGQLAKMVNQICIAGLVQGLAEAIAFGEKAGLDM
                     NQVLDVISKGAAQSWQLDNRGKTMVADKFDFGFAVDWMRKDLGLVLNEARRNGARLPV
                     TALVDQFYADVQQL"
     gene            complement(895..2031)
                     /locus_tag="PLB25_05390"
     CDS             complement(895..2031)
                     /locus_tag="PLB25_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HOE41048.1"
                     /translation="MERDQASTFITDLLKLMVGRGGSDLFLTADLPPAIKVDGKVTKV
                     SAQALNGTHTIALARSIMNDRQVAEFERTKECNFAIAPPNLGRFRVNAFLQQNKVGMV
                     LRTIPSKIPTIDSLKLPPILKEIVNSKRGLCIMVGATGSGKTTTLAAMVDHRNETTFG
                     HIITVEDPIEFVHMHKNCVMTQREVGIDTDSWGAALKNTLRQAPDVILMGEIRDRETM
                     ELAIAFAETGHLCLATLHANSTNQAIDRVINFFPEERRPQVLMDLSLNVRALVSQRLI
                     TKPDTKGRVAVVEIMINSPLISDLIFKGEVSEIKEVMKKSSNLGMQTFDQALFNAYEA
                     EQITYEDALRNADSVNDLRLQIKLNSQRGKNRDLAAGTENMTIV"
     gene            complement(2101..3144)
                     /locus_tag="PLB25_05395"
     CDS             complement(2101..3144)
                     /locus_tag="PLB25_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HOE41049.1"
                     /translation="MDITQLLAFSVKNKASDLHLSAGLPPMIRVHGDVRRINVEALDH
                     KQVHAMVYDIMNDSQRKHYEAFLEIDFSFEIEGLARFRVNAFNQNRGAGAVFRTIPSK
                     ILTLEQLNCPKIFADLALKPRGMVLVTGPTGSGKSTTLAAMVNFLNENEFGHILTVED
                     PIEFVHESKKCLINQREVGPHTQSFAAALRSALREDPDAILVGEMRDLETIRLAMTAA
                     ETGHLVFGTLHTSSAAKTIDRIIDVFPAEEKEMIRAMLSESLQAVISQTLCKTKDGQG
                     RVAAHEIMLGTSAIRNLIREAKVAQMYSSIQTGNSVGMQTLDQNLTDLVRRNVISAAE
                     ARTKAKIPENFPA"
     gene            3183..3905
                     /locus_tag="PLB25_05400"
     CDS             3183..3905
                     /locus_tag="PLB25_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HOE41050.1"
                     /translation="MTTIQSQISAVRALMADACRAAGRADDAVSLLAVSKTFDADAVL
                     AAFAAGQRAFGENYLQEAVPKITALNHLPLQWHCIGPVQSNKTRLVAEHFDWVQTVDR
                     AKIAERLSQQRPAHLPPLQVCIQVNVDGGATKSGVLPDAALALAQAVAGLPGLRLRGL
                     MCIPEPAPDFATACTVFKRARALFDAMNASGLALDTLSMGMSADLQAAIASGSTMVRV
                     GSAIFGARPHAVSETSRPVLPA"
     gene            complement(4002..4556)
                     /locus_tag="PLB25_05405"
     CDS             complement(4002..4556)
                     /locus_tag="PLB25_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 domain-containing protein"
                     /protein_id="HOE41051.1"
                     /translation="MSKARLSLMAGLLAGVALAAGAQNLKPGLWEMSTKSTGGSDEHA
                     KAMAESQKHMDSMPPEQRKKMEEMMAKHGVSLGRPGGGMSIKVCMTQAMIDRNQVSQS
                     QGNCKQTNVQRSGNTVKFSSVCTDPPATGEGVVTFSSPEAYSSKMTIHTTREGKPQTS
                     VLESSGKFLSTDCGAIKPMPMPAQ"
     gene            complement(4749..5540)
                     /locus_tag="PLB25_05410"
     CDS             complement(4749..5540)
                     /locus_tag="PLB25_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOE41052.1"
                     /translation="MFKLTSLNLNGIRSAASKGLEQWIKTSAPDCICVQEVKAQHNDI
                     HGKFHTLAGLVGYFHYAQKKGYSGVAVYTRHEPSEIVTGFASEEFDPEGRYLELRFDT
                     PTRKRSIISAYFPSGTSGDDRQQAKYRFLALMYAHLQSLKQQREFVLCGDVNIAHQAI
                     DLKNWKSNQKNSGFLPEERAWMTRLLTDGGLVDVYRQLHPTATDDAYTWWSNRGQAYA
                     KNVGWRLDYHLATPALAAGARQAEVFKGQKFSDHAPVTITYDWSD"
     gene            5551..6261
                     /gene="pyrE"
                     /locus_tag="PLB25_05415"
     CDS             5551..6261
                     /gene="pyrE"
                     /locus_tag="PLB25_05415"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HOE41053.1"
                     /translation="MSERVVAPTSPRQVQDELAQEFVQFSVDCGVLRFGQFKTKAGRL
                     SPYFFNAGLFDDGAKLGKLAQFYAHRLLASGIEFDMIFGPAYKGIPLGAALAVELARL
                     GHNRPFAYNRKEVKDHGEGGALVGAPLAGRVLIVDDVMSAGTAVRESIALIQSAGAIA
                     HAVVIALDRQEKATEGGQDVPHSAVQYVKQQLGLQVCAIARLDDLMQYLQHQSDPALL
                     DAHQRVQQYRERYGVDGS"
     gene            6258..6911
                     /locus_tag="PLB25_05420"
     CDS             6258..6911
                     /locus_tag="PLB25_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HOE41054.1"
                     /translation="MTRLTGRLPLLLLSGLVCQLHAAEAVSNTPQIYSCVDAQGRKLT
                     SDRPIRECLDREQKILNPSGTVKQKIGPVLTAQERAELEARNRAEQVERALQEEEKRR
                     NRALLTRYPTPDSHQKERVEALEHIARVKQSAASRAAQLQLDLTKLMDEMEFYKKDPT
                     KAPVKLRRQIEEVNQALASQDRFMAEQDSETKRVKDRFDEELNRLKPLWRNTLPKAL"
     gene            complement(6930..8480)
                     /gene="gatB"
                     /locus_tag="PLB25_05425"
     CDS             complement(6930..8480)
                     /gene="gatB"
                     /locus_tag="PLB25_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018716186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HOE41055.1"
                     /translation="MNTVVTENGVVVAPGQSTLVQGYEVVIGFETHAQLSTQSKIFSR
                     APTAFGAEPNTQACAVDLALPGTLPVMNKGAVERAIELGLALGSHIAPRSIFARKNYF
                     YPDLPKGYQISQFEIPVVQGGSVEFYLGNEKKSVRLVRAHLEEDAGKSLHEDFVGQTG
                     IDLNRAGTPLLEIVTEPDMRSTAEAVAYARELHKIVTWIGICDGNMQEGSFRCDANVS
                     VRKPGEPLGTRREIKNLNSFKFMQQAIDYEIRWQIEELGDGRAIQQATVLFNPDTGET
                     RAMRSKEDAADYRYFPDPDLPPLVIAPQWVQQVRAGMTELPRVMAARFCADYGLSDYD
                     ATALTQSREIASYFEAASKLCGQPKLVGNWIMGEVSRRLNQDGIEITDAPVKPETLAW
                     LVSRIHDGTISNNNAKVAFDHLWKGVNVAVASLTAPSPTLSAAGTAGKQVDDLIESLG
                     LKQMNDAGALEKIVDAVLAANPKNVGEYRGGNTRALNALVGQIMKGSQGKANPQQVNE
                     LLRQKLEA"
     gene            complement(8483..9958)
                     /gene="gatA"
                     /locus_tag="PLB25_05430"
     CDS             complement(8483..9958)
                     /gene="gatA"
                     /locus_tag="PLB25_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HOE41056.1"
                     /translation="MTQTSTDLHDLTVAQLATLLRQRKVSAVEAARHFLTRATAYKHL
                     GAFLGVSEDVTLAQASAADARLATGATGALLGVPLAHKDIFVTRDFVSTAGSKMLDGY
                     RSPFDATVVSRLAEAGAVTLGKLNCDEFAMGSANENSAFGPVKNPWDSSRTPGGSSGG
                     SAAAVAARLTPAATGTDTGGSIRQPAAFCGITGIKPTYGRASRYGMVAFASSLDQAGP
                     LARTAEDCAILLGAMCGPDPDRDSTSLDVPTEDFSLKLGDSIDGLRIGIPAEFFGEGL
                     ADDVRASVNAALKQYELLGAKLVPISLPRTELSIPVYYIIAPAEASSNLSRFDGVKFG
                     HRAAQYTNLTDMYLQTRAEGFGDEVKRRIMIGTYVLSHGYYDAYYLQAQKIRRMIADD
                     FQQAFTQCDVIAGPVAPTVAWQLGGHGNDPLADYLADIFTLPASLAGLPGMSLPVGFG
                     AGNMPVGLQLIGNHLTEARLLNVAHRFQQATDWHIARPQGV"
     gene            complement(9977..10276)
                     /gene="gatC"
                     /locus_tag="PLB25_05435"
     CDS             complement(9977..10276)
                     /gene="gatC"
                     /locus_tag="PLB25_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HOE41057.1"
                     /translation="MSLTPADIDRLAHLARLGLKPAERARMCTQLNDFFGIVEKMRAV
                     DTTGIVPLAHPVDVMGNMALRLRDDVANEPNQREANQRSAPAVEDGLFLVPKVIE"
BASE COUNT         2084 a         3001 c         3017 g         2269 t
ORIGIN      
        1 agctgctgca cgtccgcgta aaactggtcc accagggcgg ttaccggcaa gcgcgcgccg
       61 ttgcgacggg cctcattgag caccagtccg aggtccttgc gcatccagtc cacggcaaag
      121 ccaaaatcaa atttgtcagc caccatggtt ttcccacggt tgtcaagttg ccagctttga
      181 gctgcgccct tgctgatcac atcgagcacc tggttcatgt ccagaccggc tttttcgcca
      241 aaggcaatgg cttctgccag gccctgcacc agcccggcaa tgcaaatctg gttgaccatc
      301 ttggccaatt ggccggcacc gctctcaccc aggcgggtaa aagctttgga ataggcccgg
      361 gcgacgggct cggcggtctc aaaatcagcc tggtcgccac cacacatcac cgtcaaagtt
      421 ccattttggg cacccgcctg accgcctgac accggcgcat cgacgaaccc cagaccctga
      481 ctttttgcct gtgcatacag ctcgcgcgcg acgttggctg atgccgtggt gtgatccacc
      541 agcaccgcgc ccgccgacat tccggcaaac gccccatcgg caccgagcgt gacactgcgc
      601 aggtcgtcat cattgccaac gcagcaaaac accagcgcgg ccccggtggc cgcctggcgc
      661 ggtgtttgtg cctgatatat tgcgtcattt ctgactgtag cccttgtgta gtctgcacac
      721 caagctattg atttagaagc ggttcggtta tacaccgtga cgctatgtcc agcctcagcc
      781 agatgcccgg ccatcggata gcccatgaca cccagcccca gaaaggcgac acggcgcgcc
      841 gtcaccggcg tatgaatttt gttgctcatg acaggttctc cgaataaagc tagctcagac
      901 aatcgtcatg ttctcggtgc ccgccgccag gtcccggttt ttgccgcgct ggctattgag
      961 cttgatctgc agccgcagat cattgacaga atcggcattg cgcagcgcat cttcataagt
     1021 gatctgctcc gcttcatagg cgttgaacaa ggcctgatca aaggtttgca tccccaggtt
     1081 ggagcttttc ttcattactt ccttgatctc agagacctcg cccttgaaga tcaggtcaga
     1141 aatcagtggc gagttgatca tgatctcaac caccgccacc cgccccttgg tgtcgggttt
     1201 ggtgatcagg cgctgtgaca ccaaggcccg gacgttgagc gacaagtcca tcaacacctg
     1261 cggcctgcgc tcttctggaa agaagttgat aacccggtca atcgcctggt tggtgctgtt
     1321 ggcgtgcagg gtggccaggc acaggtggcc ggtttcggca aaggcgatgg ccagttccat
     1381 ggtttcgcgg tcgcggattt cgcccatcag aatcacatcg ggggcctggc gcaaggtgtt
     1441 tttcagcgct gcaccccaac tgtcggtgtc aataccgacc tcgcgttgcg tcatcacgca
     1501 gtttttgtgc atatggacga actcgatcgg gtcttcaaca gtgatgatgt gaccaaaggt
     1561 agtttcgttg cgatggtcaa ccatggccgc cagggtggtg gttttgccag agccggtggc
     1621 accgaccatg atgcacaggc cgcgcttgct gttgacgatt tctttcagga taggcggcag
     1681 tttcaggctg tcaatggtcg gaatcttgct gggaatggtc cgcagcacca ttcccacctt
     1741 gttttgctgc aaaaaggcgt tgacccgaaa gcgccccaga ttgggcggcg caatggcaaa
     1801 gttgcactcc ttggtgcgct caaactcggc cacctggcgg tcattcatga tcgagcgcgc
     1861 cagcgcaatc gtgtgcgtgc cattgagcgc ctgcgccgac accttggtta ccttgccatc
     1921 aaccttgatg gcgggcggca aatcagccgt cagaaacagg tcgctgccgc cgcgcccgac
     1981 catcagcttg agcagatcgg tgatgaatgt actggcctga tcgcgttcca tgaatgagcc
     2041 ttcgtgctgg gttaacgtga aataacttat cggtgcaggg gtggcgggtg ccgagcaaag
     2101 ctaggcggga aagttctccg ggatcttggc cttggtgcgg gcttcggctg cgctgatgac
     2161 gttgcgccgt accaggtctg tgaggttttg gtcaagcgtc tgcatgccaa cgctgttgcc
     2221 ggtctggata ctggagtaca tctgcgccac cttggcctcg cggatcaggt tgcgaatcgc
     2281 gctggtgccc agcatgatct cgtgcgccgc cacccggccc tggccgtcct tggtcttgca
     2341 cagggtttgt gagatcaccg cctgcagcga ctcgctcagc atggcgcgga tcatttcttt
     2401 ttcttcggcc ggaaacacgt cgatgatccg gtcaatggtt ttggccgcac tgctggtgtg
     2461 cagcgtgcca aacaccaggt gaccagtctc tgccgctgtc atggccagcc ggatggtttc
     2521 caggtcgcgc atttccccga ccagaatcgc gtccgggtct tcgcgcaggg ctgagcgcag
     2581 cgccgccgca aagctttgcg tatgggggcc gacctcgcgc tggttgatca ggcatttttt
     2641 cgactcatgg acaaactcga tcgggtcttc gacggtcagg atatggccaa actcgttttc
     2701 attgaggaaa ttgaccatcg ccgccagcgt ggtggacttg ccggaaccgg tcgggccggt
     2761 caccagcacc atgccacgcg gcttgagcgc cagatcggcg aaaattttgg ggcaattgag
     2821 ttgctcaagc gtcaggattt tgctgggaat ggtccggaac accgcaccag cgccacggtt
     2881 ctggttaaag gcattgaccc gaaaacgcgc cagaccctca atttcgaaag aaaagtcaat
     2941 ctccagaaaa gcctcgtaat gcttgcgctg cgagtcgttc atgatgtcat acaccatcgc
     3001 atgcacttgc ttgtgatcga gcgcctcaac attgatgcgg cggacatcgc catgcacccg
     3061 aatcatcggt ggcagcccgg ccgacaagtg cagatcggac gccttgtttt tgacgctgaa
     3121 agccaatagt tgggtaatgt ccatgaaacc cctatttgtt tgtttgttac gctcatccca
     3181 ttatgaccac gattcaatct cagatcagtg ccgtgcgcgc actgatggca gatgcctgcc
     3241 gggccgctgg ccgggcagac gatgccgtca gcctgctggc agtttcaaaa actttcgacg
     3301 ctgatgcggt gctggccgcc tttgccgctg gccaacgcgc cttcggcgaa aactatcttc
     3361 aagaagctgt gccaaaaatc accgcgctca accacttgcc gttgcagtgg cattgcatag
     3421 gcccggtaca gagcaataag acccgattgg tggctgagca ttttgactgg gtgcaaaccg
     3481 ttgatcgggc aaagatcgct gaacggctga gccagcagcg cccggcgcat ttgccaccgt
     3541 tgcaggtttg cattcaggtc aacgtcgatg gcggcgcgac caagtctggc gtgttgccgg
     3601 atgccgccct ggcccttgcg caagccgtcg cgggcttgcc cgggctgcgt ttgcgcgggc
     3661 tcatgtgcat tcctgaacct gctcctgatt ttgcaactgc ctgcacagta ttcaaaaggg
     3721 ccagagcctt gttcgatgcc atgaatgcaa gcggcctggc actcgacacc ctgtccatgg
     3781 gcatgagtgc ggacctgcag gcggcgattg cctcgggcag caccatggtg cgggtcggtt
     3841 cggcgatttt tggcgcgaga ccgcatgctg tcagcgaaac atcacggccc gtgctcccag
     3901 cctgaaaccg atttgataat atttttatag ctagcagcgc ttatttaaca agggtttgag
     3961 gcctgtttgg cctataaccg atcggttgac cggtgctgac gctattgggc aggcattggc
     4021 ataggcttga ttgcaccgca gtcagtggac aaaaacttgc cgctggattc gagcaccgac
     4081 gtctggggtt tgccctcacg ggtggtgtga atggtcattt ttgagctgta agcctcaggg
     4141 ctggagaaag tgaccacacc ttcgccagtg gctggagggt cggtacacac cgagctgaat
     4201 ttgaccgtgt tgccgcttct ttgcacattg gtctgcttgc agttgccctg ggactgggac
     4261 acctggttgc ggtcaatcat ggcctgtgtc atacagacct tgatggacat gccaccaccg
     4321 gggcggccca ggctgacacc gtgcttggcc atcatttctt ccattttctt gcgctgttca
     4381 ggcggcatgc tgtccatgtg cttttgcgat tcggccatgg ctttggcgtg ttcatcagag
     4441 ccaccagtcg atttggtgct catttcccac aggccgggtt tcaggttttg cgcacctgcg
     4501 gccagtgcga ccccagccag caggccagcc atcaggctca aacgtgcttt actcatgatg
     4561 atttcctttc aagggatgtt cattatcaac cggcttttca ccccgcccga gccaggtgtt
     4621 gcgctaccaa ctgtgttgct gcggcaaggc caaatgaaca ggatgaccac cttttcaggg
     4681 aaggcgcagt cagacaggta ttggccgagc ttgcgctaac gcctgtaccg tttgctggtg
     4741 tggccaagtc aatcgctcca gtcataagtg atcgtgacgg gggcgtggtc tgagaatttc
     4801 tggcccttga aaacttcggc ctgtcgggcc cctgccgcca gcgccggggt cgccaggtgg
     4861 tagtccagcc gccatccgac atttttggca taggcctgac cacggttgct ccaccaggtg
     4921 taggcatcgt cggtggccgt ggggtgcaat tgccggtaca catcgaccag cccgccatca
     4981 gtcagcaggc gtgtcatcca ggcccgctct tcaggcagaa aaccggagtt cttctgattg
     5041 cttttccagt ttttcaggtc gatggcctgg tgtgcgatgt tgacgtcacc acacagtacg
     5101 aactcgcgct gttgtttcag gctttgcagg tgcgcgtaca tcagcgccag aaacctgtac
     5161 ttggcctgct gccgatcgtc gccggaggtg ccgctgggga aataggcgct aatgatcgag
     5221 cgtttgcgcg tgggcgtgtc aaaccgcaac tcaaggtaac gcccctcggg gtcgaactct
     5281 tcagacgcaa aaccggtgac aatctcggac ggttcgtggc gtgtgtaaac agcgacaccg
     5341 gaatagcctt ttttttgcgc gtaatgaaaa tatccaacga gcccggccaa tgtgtgaaac
     5401 ttgccgtgga tgtcattgtg ctgcgccttg acctcttgca cacaaataca atccggtgcc
     5461 gatgttttga tccactgttc cagccctttg ctggcggctg agcgaatacc attgaggttc
     5521 aagctggtca atttaaacaa ggaattcccc atgtcagagc gggttgtcgc accaaccagt
     5581 ccccgtcagg tgcaggatga gctggcgcag gagtttgtcc agttttcagt ggattgcggt
     5641 gtgcttcggt ttggtcaatt caaaaccaag gccggtcgct tgagccccta ttttttcaac
     5701 gccggtctgt ttgacgacgg tgccaaattg ggcaaattgg cacaatttta tgcccatcgc
     5761 ctcttggcca gcggcattga gtttgacatg atctttggtc ccgcctacaa gggcattcca
     5821 ctgggtgcag cgctggcggt tgagttggcc cgcctcgggc acaaccggcc ttttgcctac
     5881 aaccgcaaag aagtcaagga ccacggcgaa ggtggcgcgc tggtgggcgc accgctggcg
     5941 ggacgggttc tgattgttga tgacgtgatg tcagcgggta ccgccgtgcg ggagtccatt
     6001 gcactgatcc aaagcgccgg tgccattgcc catgcggtgg tgattgccct ggaccggcaa
     6061 gaaaaagcca ctgagggcgg gcaggatgtc ccacatagcg cggtgcaata cgtcaaacaa
     6121 caattggggc tgcaggtctg tgccattgcc cggctcgatg atctgatgca gtacctccag
     6181 catcaaagtg acccggcctt gctggatgcc catcagcgcg tccagcagta ccgcgaacgt
     6241 tatggagtcg atggatcatg acgcgcttga ccggccgcct gccattgttg ctgctgagtg
     6301 gcctggtgtg ccaactgcat gctgcagaag ccgtcagcaa cacgcctcag atctattcct
     6361 gtgtggatgc gcagggtcgc aaactgacct ccgaccgccc gatcagggag tgtcttgacc
     6421 gtgaacagaa aattctcaat cccagcggta ctgtaaagca aaaaattggc cccgttctaa
     6481 cggcccagga gcgcgccgaa ctggaggcca ggaacagggc cgaacaggta gaacgggcac
     6541 ttcaggaaga agaaaagcgc cgcaaccggg cgctattgac gcgctatccc accccggatt
     6601 cacaccagaa ggagcgggtc gaggcacttg agcatatcgc ccgtgtcaag cagagtgctg
     6661 cgtcccgggc cgcccaactg cagcttgact tgaccaaatt gatggatgag atggagtttt
     6721 acaaaaagga ccctaccaag gcgccggtaa agctgcgccg ccagatcgaa gaagtgaatc
     6781 aggcattggc atcgcaggat cgcttcatgg cagagcagga ctcggaaacc aaacgcgtca
     6841 aagatcgttt tgatgaagag ttaaaccggc tcaagccact ttggcgcaac accttgccca
     6901 aggccctttg actgggattt ggcgctcgtt cacgcttcca gtttctggcg cagcagctca
     6961 ttgacctgtt gcgggttggc cttgccctgg ctgcctttca tgatttgccc gaccagcgca
     7021 ttaagcgccc gggtgttgcc gccacggtat tcgcccacgt tttttgggtt ggcagccagc
     7081 acagcatcaa caattttttc cagcgcgccg gcgtcgttca tctgtttgag acctagcgat
     7141 tcaatcaagt catcgacttg cttaccagca gtacctgcag cgcttagggt tggagacggc
     7201 gcagtcaaac tcgcaacagc aacgttcacg cctttccata aatgatcgaa agcgactttt
     7261 gcattgttgt tggaaatcgt gccatcatga atacggctga ccaaccatgc caacgtttcc
     7321 ggcttaactg gcgcatctgt aatttcgatg ccatcctgat tgaggcgacg cgagacctcg
     7381 cccatgatcc agttgccaac cagcttgggc tgcccgcaaa gcttgctggc cgcttcaaaa
     7441 tatgaagcaa tctcgcggct ctgggtaagg gctgtcgcgt cgtaatcaga taagccgtaa
     7501 tctgcgcaga agcgcgctgc catgacacgt ggcagttcgg tcatccccgc ccgcacttgc
     7561 tgaacccact gcggtgcaat caccagcggc ggcaaatccg ggtcggggaa atagcggtaa
     7621 tctgccgcat cttccttgct gcgcatggcg cgggtttcac cggtgtccgg gttgaataac
     7681 accgtggcct gctggatggc gcggccatct ccaagctctt caatctgcca gcgaatctcg
     7741 tagtcaatcg cctgctgcat gaatttgaaa ctgttgaggt tcttgatttc gcgtcgcgtg
     7801 cccagaggtt cgccgggctt gcgcaccgac acgttggcat cacagcggaa cgagccttct
     7861 tgcatgttgc cgtcgcaaat gccgatccaa gtcacgatct tgtgcagttc cctggcataa
     7921 gccactgcct cggcggtgga tcgcatgtcg ggctcggtga cgatctccag cagcggcgtg
     7981 ccggcgcggt tcaggtcgat gccggtctgg ccaacaaaat cttcgtgcag cgacttgccg
     8041 gcatcttctt ccagatgcgc gcgcaccagt cgcaccgact ttttctcatt gcccaggtaa
     8101 aactcaactg agccgccttg caccacggga atttcgaact gactgatctg gtagcccttg
     8161 ggcagatcag gataaaagta attctttcgg gcaaaaatgc tgcgcggcgc aatgtgggag
     8221 cccagcgcca ggcccaattc aatcgcccgc tcaaccgcac ctttgttcat caccggtagc
     8281 gtgccgggca aggccaagtc cacggcgcag gcctgcgtgt tgggttcggc accaaaggcg
     8341 gtcggggcgc ggctgaaaat cttggattgg gtggagagct gggcgtgggt ctcgaaaccg
     8401 atgaccactt cgtagccttg caccagcgtg gactggcctg gtgctacaac aacgccattt
     8461 tcagtgacaa cagtgttcat ggtcagactc cttgcggtcg ggcaatgtgc caatcggtgg
     8521 cttgctgaaa gcggtgcgcc acattgagca ggcgtgcctc tgtcaggtga ttgccgatca
     8581 gttgcaggcc gaccggcata tttcccgcgc caaaacccac cggcaggctc atgccgggca
     8641 ggccggcaag agaagctggc aaggtgaaga tatcggccag atagtcggcc agcgggtcat
     8701 tgccatggcc accgagctgc caggccacgg tgggcgctac cggtccggca attacgtcac
     8761 actgtgtgaa ggcctgctga aaatcatcgg caatcatgcg gcggattttt tgcgcctgca
     8821 aatagtaggc atcgtaataa ccgtgcgaca gcacataggt gccaatcatg atgcggcgct
     8881 tgacctcgtc accaaaaccc tcggcgcggg tttgcaggta catgtcggtc aggttggtgt
     8941 actgcgctgc gcgatgacca aacttgacac cgtcaaatcg actcaggttg cttgatgcct
     9001 cagccggtgc aatgatgtaa tagaccggaa tcgacaactc agtgcgcggc agcgagatcg
     9061 gcaccagctt cgcacccagc agctcatatt gtttgagcgc ggcattcacg ctagctcgca
     9121 catcatcggc gagcccttca ccaaagaact cagcggggat accaatgcgc aagccgtcaa
     9181 tggagtcgcc cagcttcaaa ctgaagtctt cagtcggcac gtcaagcgag gtcgaatccc
     9241 gatcagggtc cgggccgcac atggcgccca acaggatggc gcagtcttct gccgtgcggg
     9301 ccaatggccc ggcctgatcc aggctggaag caaacgccac catgccgtag cgcgaggccc
     9361 gcccataggt tggcttaatg ccggtgatgc cgcaaaacgc ggcgggctgg cggatcgagc
     9421 cacccgtgtc tgtgccagtc gccgcagggg tcagacgtgc cgccactgcc gccgcgctgc
     9481 cacccgatga gccgcctggg gtgcggctgc tgtcccacgg gtttttgacc gggccaaagg
     9541 ccgaattctc attggctgag cccatggcga attcgtcgca attgagcttg cccagggtca
     9601 cggcaccggc ttcggccagg cgcgatacca cggtggcatc aaagggtgag cggtaaccgt
     9661 cgagcatctt cgatccggcc gtggagacaa agtcacgggt gacaaaaata tccttgtgtg
     9721 ccaatggcac gcccaacagc gctccggtcg cgccggttgc cagccgggca tctgccgcgc
     9781 tggcttgtgc cagcgtgaca tcttcagata cacccagaaa agcgccaagg tgcttgtacg
     9841 cagtggcacg ggtcagaaaa tgccgggcag cctccaccgc agaaaccttg cgctggcgaa
     9901 gcagtgtggc cagttgggcc accgtcaaat catgcagatc ggttgatgtt tgcgtcatga
     9961 tgaaaatgaa attgccttac tcaatgactt tgggcaccag aaacaagcca tcttccacgg
    10021 caggggcgct gcgctggttg gcttctcgct ggtttggttc gttcgccacg tcgtcgcgca
    10081 ggcgcaacgc catgttgccc atcacgtcca ccggatgcgc cagcggcacg atgccggtgg
    10141 tgtcaacggc gcgcattttc tcgacaatgc caaaaaaatc attgagttga gtgcacatgc
    10201 gcgcacgctc agcaggcttg agaccgagcc gggccagatg ggccagccgg tcgatatcgg
    10261 cgggggtcag cgacatggtt tattcaatcg aaacaaggtg taaaaggatg cctgtgcaga
    10321 gccgcagcac cggcggctca taggcaatgg ggtattatcc cgcctttggg c
//
LOCUS       DAOSLL010000085        10222 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6387_length_10222_cov_4.430215, whole genome shotgun sequence.
ACCESSION   DAOSLL010000085 DAOSLL010000000
VERSION     DAOSLL010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10222
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6387_length_10222_cov_4.430215"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..932)
                     /locus_tag="PLB25_05440"
     CDS             complement(204..932)
                     /locus_tag="PLB25_05440"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HOE41058.1"
                     /translation="MHREYHRWYSHRLQRDMELLVFGHAGAKAMVFPTRDGSFHEYED
                     LRIVHSLAEKINAGQLQLWCLDSVAWETFYCRWRHPAERIRRHAQYEEYVLNEVMPLM
                     QQQNPHPCTIAHGCSLGAFFAANMAFRHPQHFQKLAAFSGRYDLTMSVEAFHDLLGGY
                     YDENVYFHTPSHFLPNLDCDWRLAHLRRMDIVMVVGGEDPFLHNNRQLSDILHAKGVP
                     NQLHVWDGRAHQGHYWRRMASLYV"
     gene            complement(955..1923)
                     /locus_tag="PLB25_05445"
     CDS             complement(955..1923)
                     /locus_tag="PLB25_05445"
                     /inference="COORDINATES: protein motif:HMM:NF017280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HOE41059.1"
                     /translation="MHPPQFTHSYAFDPSYNMDLAQLLAIRAPEPPADFANFWRQRHA
                     AALGLLPRPRIKLAALQRPHHRVYDLHFVSTDAVEIGGWLLTPKDGQVRHGIVFGHGY
                     GGCELPQELLPINGAVQLFPCFRGIGRSPVAGVSPDPNFHVLHNIQDRQRYILGGCVE
                     DLWLAVSALLELFPEVAGRVAYSGISFGGGIGALALPWDSRVARLHLQVPTFGHQALR
                     LTLPCIGSGESVRQFQRQHSFHVIDTLAYYDAASAVQHLRIPALVAPALFDPAVPPPG
                     QFAIYNAIAAPLKRLFVLSAGHFEHAGQQQELREMNRELASFFMES"
     gene            complement(1969..>2707)
                     /locus_tag="PLB25_05450"
     CDS             complement(1969..>2707)
                     /locus_tag="PLB25_05450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOE41060.1"
                     /translation="AGRLGGDEFVALLPDLPHGDAALAVAEKIRLALVEEFMTEQGVV
                     LQVSASIGIALYPDHAQTEKDLLRLGDEAMYQAKRSGRNAVVLCNAAAQAAVPPNIVI
                     NQAPFIVHLRWKPAFSCGHPVIDQEHQALFDLANNLIDCAALQPPQPPQAFAAAFNSL
                     LSHTKSHFSHEEALLRELGSVGLTEHAALHQVLLAQAQALHEELRGEPGSPAAMQALL
                     KFLATEMVANHVLHADRAIFAAFPRKS"
     assembly_gap    2708..2717
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2718..4455)
                     /locus_tag="PLB25_05455"
     CDS             complement(<2718..4455)
                     /locus_tag="PLB25_05455"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE41061.1"
                     /translation="MNLRRFQPQSLVVRLTLLTLVIVVLTFFVLSSYIRSLMREELLR
                     FTGEQQRSALAVLVSQVSHSLQERLDTLGTVAARITARDMENPMAAQAFLLERPFLAK
                     VFNGGVVLWSRTGQRLTDAQFEGMSAVNQPLDPQELTRLLSDGQPLVGRVHRDAQLQV
                     GVFAIAVPVRDRQGQVIGALGGTIRLDQPNFLSQLLHHPYGQTGHFFLIDAHDRLIFA
                     SSDSTRLMEVLPAPGQHPMIDKFVAGFDGTVQTVNPHGVSLLVSVKPVAPAQWYASVT
                     LPTDEVFALISAIKWRTRLAGLGLLGLLVLLVGSMVRRQLAPMTAAVHTMAGFVHQNQ
                     PPQALPVARPDEVGELVGGFNQLLDTLAQQQKALQRSELFKQAVLNSVTAEIAVLDAQ
                     GVIVTVNEAWHNQRCANAESTPGANYLVACRSMANHEVTQAGQMTAEQGIRAVLAGQL
                     SRFYLEMPHHTPALRRWCSMSVTPMAQGEQRGAVVSLEDISERIEAQNQVRELAFYDP
                     LTRLPNRRLVSERLAQEMARARRAKTRLAMIFIDLDQFKPVNDTLGHEVGDWLLQALA
                     QRILLCLRASDTA"
     gene            complement(4869..6830)
                     /locus_tag="PLB25_05460"
     CDS             complement(4869..6830)
                     /locus_tag="PLB25_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOE41062.1"
                     /translation="MSIHTASADIAITSSETTAGDDLPWDREVCLGLIDQLWSLRHTM
                     LAREAQLQIELSSVPPRHQNSARNLIHYLCLRTVDLRPLQEQLAKLGLSSLGRSESHV
                     LANLDKVLGVLHRLTDQVWQDRSGEEPVGSVSSRAHLMEHTASLLGPAPANRPVRIMV
                     TLPSEAARDFAVVRDLVDAGMDIARINCAHDGPQDWKAMALHVRRAAQLMQRPIKILM
                     DLGGPKIRTGEMAPMAPVLKLKPSKDQFGRVFRAARLHLRPIDSLENIDDVDACIGVW
                     EVWLERLKPRTSIEFTDARGAKRHLMVVHTDAKGAIAESLQTAYLTPETVLTIGGAHG
                     KKKHSTLVCQIDSQPGTLHLHRGDMLHLTDGSQTSSAADDDTHDLGQDIAHITCTLPQ
                     VIHQVKPGARIWFDDGRIGGIVRHSFPNYLSIEITQARDSGEKLSGDKGINLPDSQLD
                     LPALTPKDLEDLQTVVTEADMVGLSFVQRPADVDQLRRMLAQLGRADMGLVLKIETLQ
                     GFENLPELMLSAMAASSAGIMIARGDLAVECGYERMAEVQEEILWCAEAAHMPVIWAT
                     QVLESMAKTGLPSRAEISDAGLGERAECVMLNKGPYIVQAIHTLDNILKRMGGHQDKK
                     RPLLRALRAWGDNPTLLLQQAPQADVKLI"
     gene            6962..7216
                     /locus_tag="PLB25_05465"
     CDS             6962..7216
                     /locus_tag="PLB25_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41063.1"
                     /translation="MLTFPRCSTTVQRKMSRHLNNLEALCNKLQQRYGSDDPIFLEVK
                     TDLEAEMLHSPRAAMRHDWSVPYRTLILQQRNATLAQSSR"
     gene            7564..7809
                     /locus_tag="PLB25_05470"
     CDS             7564..7809
                     /locus_tag="PLB25_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41064.1"
                     /translation="MTQQAENTSGVDNPTPKANWVTILNRLVTNIALQTSTLLLPGII
                     TWLRRRPMMKVTATPLIAMLALPIVFGPMVCSTPKTL"
     gene            7980..>10222
                     /locus_tag="PLB25_05475"
     CDS             7980..>10222
                     /locus_tag="PLB25_05475"
                     /inference="COORDINATES: protein motif:HMM:NF019874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOE41065.1"
                     /translation="MFTLRPKAFAPRLFTRVFLGTGALVISLLVAIYVTAIPLVESAM
                     RAAEERAGLTIMDNVHQLLEMAEEDLKQYRIAMTEERKRKLKDIVSLTEAGLRFQYLD
                     ALAHGLSKSAALDRTVGGLRVSRYGGDDYVWAFDNSSVLIAHPDPALDRRDFSQVQDV
                     DGHLIIPQMVKLGLEKGGGFFSYHWHRLGRSAQAEKLAYTRHLSDLGIHIATGIYVDD
                     IEELVAVRKAKLMRQLAARIANIRIARDGYVYVFDGKYNMLFHPNLAGKNLGQTKNPV
                     TGKLILDELMGVTDSPNGWRYLWDKPGDEQHFVHRKISWVRHFPSYDWYIVASVYLDD
                     LYESADALKHRLVMFGLIGLALALCLAFVFVRRFTHPIVQLAEVARRVENGDPDATSS
                     IRRSDELGDLAHAFNRMVTQLRDHIQNLDEKIRARTLQLAESHAQIAAQNAGLEQRVQ
                     ERTQEAELARQAAIAASLAKSEFLANMSHEIRTPMNAILGMAYLALKTELNVRQRDYI
                     SKIHAAGSALLGIVNDILDFSKIEAGKLDIENIDFNFLETIDHVTSLMALRVQDKDLE
                     LLIDIDRAVPANLRGDPLRLGQVFTNLIGNAVKFTPQGSIRLRAEVLERTADRVNLRF
                     AVQDTGIGMTPDQIDLLFQAFSQADSSTTRKYGGTGLGLAISKRLVNMMGGELQVEST
                     VAVGSTFTFSVWLGTGHEDLRTLPESVLGLHVLAVDDSQSSLEVMQTYLADLGLRVDL
                     AVSGAEGL"
BASE COUNT         2145 a         3059 c         3004 g         2004 t
ORIGIN      
        1 gtggcactgc ggtcgctcat gcccagcatg gttttgaagt cgccgcgcgc catttcctca
       61 ccgcttaaaa acaggtagtg cagaccacgc aatgccgcct ggcgcacgcc gattttcagc
      121 aggttggcct caaagaccag gcaggctttg atgcgccttc acctgcgcaa acgacctgtc
      181 agactcaatc tggcgcagca aaactacacg tacaacgatg ccatgcgccg ccagtaatgg
      241 ccttgatggg cgcgaccgtc ccagacatgc agttggttgg gtacgccctt ggcgtgcagg
      301 atgtcactca actgccggtt gttgtgcaag aacggatcct cgcctccgac caccatgacg
      361 atgtccatgc ggcgcaggtg cgccagtcgc caatcacaat cgaggttggg cagaaagtgg
      421 ctgggagtgt ggaagtaaac gttttcgtcg tagtagccgc cgaggaggtc gtgaaacgct
      481 tccacgctca tggtcaggtc gtagcgcccg gagaatgccg ccagtttctg aaagtgctgc
      541 ggatggcgaa acgccatgtt ggcggcaaaa aaagccccca ggctgcaacc gtgggcaatg
      601 gtgcaggggt gcggattttg ctgctgcatc agcggcatca cctcattgag gacgtattcc
      661 tcatactgtg cgtgacgccg gatgcgctcg gccgggtgac gccagcggca atagaaggtt
      721 tcccaggcga cgctgtcaag gcaccacaac tgcaactgcc cggcgttgat tttctcggcc
      781 agactgtgca cgatgcgcaa atcttcgtat tcgtgaaagc tgccgtcgcg ggtcggaaaa
      841 accatggcct tggcaccggc atgaccaaat accagcaact ccatgtcgcg ctgcaaacgg
      901 tggctgtacc agcggtgata ctctcgatgc atacgcgaat gcctccatag catgtcatga
      961 ttccatgaaa aagctggcaa gctcccggtt catttcccgc aactcttgct gctggccggc
     1021 atgctcaaaa tgaccggccg acagcacaaa caaacgtttg agcggcgctg cgatggcgtt
     1081 gtaaatggca aactgcccgg gcggcgggac cgccggatcg aacagcgccg gtgccaccag
     1141 agcgggtatg cgcaggtgtt gcacagcaga tgcggcatcg taataagcca atgtgtcgat
     1201 tacgtgaaaa ctgtgctgcc gctgaaattg gcgcaccgac tcaccgctgc caatgcaggg
     1261 cagagtcagg cgcagcgcct ggtggccaaa agtgggcact tgcagatgca agcgcgcgac
     1321 ccggctgtcc cagggcagcg cgagcgcgcc aatgccgccg ccaaagctga tgcccgaata
     1381 cgccacccgg cctgccactt cgggaaagag ttccagcagg gccgacaccg ccagccacag
     1441 gtcttccacg cagccaccga ggatgtagcg ctgtcggtcc tgaatattgt ggaggacatg
     1501 gaagttgggg tctggcgaaa cacccgccac cgggctgcgg ccaatgccgc gaaagcacgg
     1561 gaaaagctgc accgcgccat tgatcggcaa cagttcctgc ggcaactcgc agcctccgta
     1621 gccatgcccg aagacaatgc catggcgcac ctggccatcc ttgggggtca gcagccagcc
     1681 accgatttcg accgcatcgg tggagacaaa gtgcaggtcg tagacccggt ggtgcgggcg
     1741 ctgcaaggca gcgagcttga tgcgtggtcg tggcaataga cctagcgcag cagcatggcg
     1801 ctgtcgccag aagttggcaa agtcagccgg tggctcgggc gcgcgaatcg ccaggagctg
     1861 cgccagatcc atgttgtagc tgggatcgaa cgcgtagcta tgggtgaact ggggtggatg
     1921 catggcggaa atcgaaaagg tgaaaccgta acgcccaaca ataaatggtc aggatttacg
     1981 gggaaatgcg gcaaagatgg cccggtcggc gtgcagaaca tggttggcga ccatttcagt
     2041 tgccaggaat ttcagcagcg cttgcatggc agccggactg ccgggctcgc cgcgcagttc
     2101 ctcatgtaac gcctgggcct gggccagcag gacttgatgc aaggcagcat gttccgtcag
     2161 gccgacactg cccagttcgc gcagcagcgc ttcttcatgc gaaaaatgcg atttggtgtg
     2221 gctcaggagc gagttgaaag ccgccgcaaa cgcctgcggc ggctgcggcg gctgcaaggc
     2281 ggcgcagtca atcagattat tggccaggtc aaacagtgcc tgatgttcct ggtcgatcac
     2341 cgggtgacca caggagaagg caggtttcca gcgcagatgc acgataaacg gtgcctggtt
     2401 gatcacgatg ttcggcggta cggcggcttg tgccgccgca ttgcacagca ccacggcatt
     2461 acggccgctc cttttggcct gatacattgc ctcatcaccc aggcgcagca agtctttttc
     2521 ggtctgtgca tggtcgggat acagggcgat accgatgctg gctgaaacct gcaacaccac
     2581 accttgctct gtcatgaact cctcaaccag tgccaggcgg atcttttcgg ccacagccaa
     2641 cgccgcatcg ccatgaggca agtcaggcag cagtgccaca aattcgtcgc cacccaggcg
     2701 cccggcgnnn nnnnnnncgg cggtgtcaga ggcgcgcaaa cacaacaaaa tgcgctgcgc
     2761 caaggcttgc agcagccagt cgcccacctc atggccgagc gtgtcgttga ccggcttgaa
     2821 ctggtccagg tcaatgaata tcatggcgag ccgggttttt gcgcggcggg cgcgggccat
     2881 ttcctgcgcc agccgctccg acaccaggcg gcggttgggc aggcgggtca gcgggtcgta
     2941 aaaagccaat tcccgaacct ggttctgcgc ctcaatgcgc tcgctgatgt cttccagcga
     3001 caccacagca ccacgctgtt cgccttgcgc catcggggtc acgctcatgc tgcaccagcg
     3061 tcgcaaggca ggcgtgtggt ggggcatttc aaggtagaaa cgggacaatt gtccagccag
     3121 caccgccctg atgccctgtt ctgcggtcat ctgcccggcc tgggtcacct cgtggttcgc
     3181 catgctgcga cacgccacca ggtagtttgc acccggtgtg gactcggcat tggcgcagcg
     3241 ctggttgtgc caggcttcgt tgacggtcac gatcacgccc tgggcatcga gcacggcaat
     3301 ttctgccgtg accgagttga gcaccgcctg cttgaacaat tcgctgcgct gcaaggcctt
     3361 ttgttgctgt gccagggtat cgagcagttg gttaaagccg cctaccagtt cgcccacctc
     3421 gtcgggcctg gctaccggca aggcctgcgg cggctgattt tggtgcacaa agccggccat
     3481 ggtgtgcacg gcggcagtca tgggcgcaag ctggcggcgc accatcgagc ccaccagcag
     3541 caccaacagg cccaacaaac ccaggccagc cagccgggtg cgccacttga tcgccgaaat
     3601 cagggcaaag acttcatcgg tcggcagagt gaccgaggca taccattggg ccggtgcgac
     3661 cggcttgacg ctgaccagca gcgacacgcc gtgcggattg accgtctgca ccgtaccgtc
     3721 aaagccagcc acaaatttgt cgatcatcgg gtgctggccc ggcgccggca gcacctccat
     3781 caggcgcgtg ctgtcagagc tggcaaaaat cagccggtca tgggcatcaa tcaggaagaa
     3841 gtgcccggtt tggccatagg ggtgatgcag caattggctc aaaaaattgg gttgatcgag
     3901 ccggatcgtg ccgcccagcg caccaatgac ctggccttgg cggtcgcgca cgggcacggc
     3961 aatcgcaaac acgccgacct gcaactgagc atcgcgatga actctgccta ccaagggctg
     4021 cccatcgctg agcaggcgcg tcagttcttg cggatcaagc ggctggttga cggcgctcat
     4081 gccctcaaat tgcgcgtcgg ttagccgctg gccggtacgg ctccatagca ccacgccgcc
     4141 attgaatacc ttggccaaaa atgggcgctc cagcaaaaat gcctgcgcgg ccatgggatt
     4201 ttccatatcg cgggctgtga tgcgtgcggc caccgtgccc agggtgtcca gccgctcttg
     4261 caggctatgg ctgacctggc tgaccaaaac cgccagcgcc gagcgctgct gctctccggt
     4321 aaagcgcaac aattcctcgc gcatcagcga cctgatatag ctcgacaaca cgaaaaatgt
     4381 cagcaccaca atcaccagcg tgagcaaagt caggcggacc actagcgact ggggctgaaa
     4441 acgccgaagg ttcatctcaa gggtattcgt gtgttgctgt aactcgcgaa gtggctatgc
     4501 tcaagcttgg gcaggcgttg cgccagggca agcagcaaat cgaaacatac cgactcgcca
     4561 tcgcgtagag cagcgacctg atgatcaatg atcccatgaa ttgccacgtc gctgacgctc
     4621 ctcgcattta cgcaagtcgg tagattggca atttccatga ctgcctgatc cggacgaccc
     4681 aaaatcgaac agccttcgtc gcgagggcgc aactgttgca gggcaaccgg gtcgctggtg
     4741 tttgcaggct tgtgtacctg ttcctggaaa tcaccgcaca ggacctgtca gtagagatta
     4801 tgatgctatt aacaatatag cttactgcgc tttatccata agggatagag gcataaaatg
     4861 caaataaact agatcaactt gacatccgcc tgcggggctt gttgtagtag caaagtgggg
     4921 ttgtcgcccc acgcgcgcag ggctcgcagc aaggggcgct ttttgtcctg gtgcccgccc
     4981 atgcgtttca gaatgttgtc gagcgtgtga atggcctgga caatgtacgg ccccttgttg
     5041 agcatcacac actcggcgcg ttcccccaaa ccggcatccg aaatctcggc gcgcgagggc
     5101 agtccggttt ttgccatcga ttcgagcacc tgggtcgccc agatgaccgg catgtgggcg
     5161 gcttcggcgc accatagaat ttcttcctga acctcggcca tgcgctcata gccgcattcc
     5221 accgccaggt caccgcgcgc gatcatgatg ccggcactgc tggccgccat ggcgctaagc
     5281 atcagttcgg gcaggttttc aaatccctgc agggtttcta ttttgagtac cagacccatg
     5341 tcggcccgac ccagttgcgc cagcattcgg cgcaattgat ccacatcggc aggcctttgc
     5401 acaaacgaca agcccaccat atccgcttcg gtgacaacgg tttgcaagtc ctccaggtct
     5461 ttgggggtca gcgctggcag atcgagttgg ctgtcaggca gattgatgcc tttgtcaccc
     5521 gagagctttt cacccgaatc acgcgcctgg gtgatttcga tgctcagata gtttgggaag
     5581 ctgtgccgca cgatgccacc aatgcgacca tcgtcaaacc agatgcgcgc acctggcttg
     5641 acctgatgaa tgacttgcgg caatgtgcag gtgatgtgcg caatatcctg acccagatca
     5701 tgcgtgtcgt catccgctgc gctggatgtc tggctgccgt cggtcaggtg caacatgtcg
     5761 ccacggtgca ggtgcagggt tcccggctgg ctgtcaattt ggcacaccag tgtcgagtgc
     5821 ttctttttcc catgggcgcc gccaatggtc aacactgtct caggtgtcag gtaggcggtc
     5881 tgcagacttt cggcgatggc acctttggca tcggtgtgaa ccaccatcaa gtggcgcttg
     5941 gcaccgcgcg cgtccgtgaa ctcgatgctg gttcgcggct ttagccgttc cagccagacc
     6001 tcccagacac caatacaggc atccacatca tcgatgtttt caaggctatc gatcggtcga
     6061 agatgcaaac gggcagcccg aaagacacgg ccaaattggt ctttggacgg tttgagcttg
     6121 agcaccggtg ccattggtgc catctccccg gtgcgaatct tggggccacc cagatccatc
     6181 agtatcttga tcggacgctg catcaattgc gccgcgcgcc gaacgtgcaa cgccatcgcc
     6241 ttccagtctt gcgggccgtc atgcgcacaa ttgatgcggg cgatatccat accggcatcg
     6301 accaggtctc tgacaacggc aaagtcccgg gctgcctccg agggcagggt caccatgatg
     6361 cgcaccggac ggtttgccgg tgctggccca agcaaactgg ccgtgtgctc catcaggtgg
     6421 gcgcggctgc tgacgctacc gaccggctcc tcgccagacc ggtcctgcca gacctggtcc
     6481 gtcagtcggt gcagcacacc cagcaccttg tccaggtttg ccaatacatg cgattcggat
     6541 cgacccagcg aggacaagcc cagcttggcc aattgctctt gcagcggacg cagatcaacc
     6601 gtgcgcagac acaaatagtg aatcaggttg cgggcgctgt tctggtgccg gggtggcacg
     6661 ctgctgagtt cgatctgcaa ctgtgcttcc cgcgccagca tggtgtggcg cagcgaccac
     6721 aactggtcga tcagaccaag gcaaacctcc cggtcccaag gcaagtcatc gccagcggtg
     6781 gtttcagagg atgtgatggc tatgtcggca gatgcggtat ggatgctcat ggtggggtta
     6841 tatgatcttt atgtgaccaa ataatgacac accaattgct tttcggatgt caattgaaat
     6901 gcgcggcgtg tttgctttaa gtcaactcca tgatgattag cgtcatgaaa gcagcaatcg
     6961 gttgttaaca ttcccccggt gttcaacaac ggttcaacgc aaaatgtcac gtcatctcaa
     7021 caatctggaa gcgctttgca acaagttgca gcagcgttat ggttcagacg atcccatatt
     7081 tcttgaagtt aagacagatc tggaagctga aatgctccac tcgccaaggg cggctatgcg
     7141 tcacgactgg tcggtaccgt accgcaccct gatcttgcaa cagcgcaacg cgacacttgc
     7201 acaaagcagc cgctgacttt cttggactcc gttgcccccc ggacatgaaa gttgtttcac
     7261 gttaacccaa gcccttgggc agcatgacgg catcatcgaa tcaagtgtca gacctgctca
     7321 aaaggtagcc gcgggttacg gacatggtcc ataaccacag catgccggtc cagcaggttg
     7381 tggcaactct ggcacaacgc cagcatctgg ttgaacggca aacctgacgg gtctttttcg
     7441 gcgctccaca gtacgcatgc cctggtggcg aagcagcttt gtcatcaatt cttcacgcca
     7501 gcgtgccaca ctggcactgg ttaaccggca cggtgccgcg cgcaatcaac tcgcgagaac
     7561 cccatgacgc aacaagctga aaacacatct ggagtcgata accctactcc gaaggcgaac
     7621 tgggtgacga ttttgaatcg gttggtgacc aacatcgcgc tgcaaaccag cactttgctg
     7681 ctgccgggca tcatcacctg gctgcggcgg cggccgatga tgaaggtgac tgcgacaccg
     7741 ctaatcgcca tgcttgcctt gcctatcgtc tttggcccga tggtgtgcag cacgccgaaa
     7801 acgctgtgat ggacagcgaa agccacggcg atgtgatcgc aagcggcctc tgaagtcaaa
     7861 gctgcgtttt ggccggttgg ctttgggccg tttcggcttg cttgccagtt gtctcgacct
     7921 tcaggaagcc cgccgcggcg aagaacaact cctgtcacat tgactgacca cgatgagcca
     7981 tgtttacgct cagacccaaa gccttcgcgc cacgcctgtt cacccgtgtt ttcctgggca
     8041 ctggtgcgct ggtcatcagc ttgttggtgg cgatctatgt gactgccata cccctggtgg
     8101 agtcggccat gcgcgccgcc gaagaacgcg ccggactgac catcatggac aacgtgcacc
     8161 agttgctgga gatggccgag gaagacctca agcaataccg catcgccatg accgaggagc
     8221 gcaagcgcaa gctcaaggac attgtttcgc taaccgaggc cgggctgcgt tttcagtacc
     8281 ttgatgccct ggcgcacggt ctgagcaagt cggcggcgct ggatcgcacc gtcggcggct
     8341 tgcgcgtgtc acgctacggc ggcgacgact atgtgtgggc tttcgacaac agctcggtac
     8401 tgatcgccca ccccgacccg gcgcttgaca gacgtgactt ctcgcaagtt caggatgtcg
     8461 atggtcatct gatcattcct cagatggtca agctcggcct ggaaaaaggc ggcggctttt
     8521 tttcttacca ctggcaccgc ctgggaagaa gtgcacaggc cgagaagctg gcctacaccc
     8581 ggcatctgag cgatctgggc atccacattg cgaccgggat ttacgtcgac gatatcgagg
     8641 agctggtggc agtgcgcaag gccaagctga tgcgccagct tgccgcccga atcgccaaca
     8701 tccgcatcgc ccgcgacggt tacgtttatg tgtttgacgg caagtacaac atgctgtttc
     8761 accccaacct ggccggcaag aacttgggtc aaaccaagaa cccggtcact ggcaaactca
     8821 ttctggacga gttgatgggg gtcaccgaca gcccgaacgg ctggcgctac ctgtgggaca
     8881 agcccggtga cgaacagcat tttgtccacc gcaagatctc ctgggtgcgc catttcccca
     8941 gttacgactg gtacatcgtg gcctcggtgt atctggacga cctgtacgaa agcgccgatg
     9001 cgctcaaaca ccggctggtc atgttcggac tgatcggact ggcgctggcg ctttgtctgg
     9061 cgtttgtatt tgtgcgacgt ttcacccacc cgatcgtaca actggctgag gtggcaaggc
     9121 gggtggaaaa cggcgacccc gatgccacca gcagcatccg acgcagcgac gaactcggcg
     9181 atctggccca cgccttcaac cgcatggtga cgcaactgcg tgaccatatt caaaatctgg
     9241 atgaaaaaat ccgcgcacgt acgctgcaac tggccgaaag ccacgcccag atcgccgcgc
     9301 aaaacgccgg gctggagcag cgcgtgcaag agcgcaccca ggaagccgag ctggcccgcc
     9361 aggccgccat tgccgccagc ctggcaaaga gcgaatttct ggccaatatg agccacgaga
     9421 tccgcacgcc gatgaacgcc attctgggca tggcctatct ggcgctcaag accgaactta
     9481 acgtccgtca gcgcgattac atcagcaaga ttcacgctgc cgggtcggcg ctgctgggta
     9541 tcgtcaacga catcctcgat ttttcaaaga tcgaagccgg caaacttgac atcgaaaaca
     9601 tcgacttcaa cttcctcgaa accattgacc acgtcaccag cctgatggca ttgcgggtgc
     9661 aggacaaaga tctggagttg ctgatcgaca tcgaccgggc ggtgccagcc aacttgcgcg
     9721 gcgacccgct gcggctgggc caggtattta ccaacctgat cggcaacgcc gtcaagttca
     9781 ccccgcaggg cagcatccgg ctgcgggccg aggtacttga gcgcactgcc gaccgggtaa
     9841 acctgcgctt tgcggtacag gacaccggca tcggcatgac acccgaccag atcgacttgc
     9901 tgtttcaagc cttctcccag gccgacagct ccaccacccg caagtacggc ggcaccggtc
     9961 tgggactggc catcagcaag cgcctggtca acatgatggg tggcgagctg caggttgaga
    10021 gcacggtcgc agtgggcagc acctttacct tctcggtctg gctgggcacc ggccacgagg
    10081 atctacgcac cctgcccgag agcgtgctgg gtctgcatgt gctggcggtg gacgacagcc
    10141 aatcctcgct cgaggttatg cagacctatc tggccgatct tggtctgcgg gtcgacctgg
    10201 ccgtcagcgg tgccgaggga ct
//
LOCUS       DAOSLL010000086        10192 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6406_length_10192_cov_4.212489, whole genome shotgun sequence.
ACCESSION   DAOSLL010000086 DAOSLL010000000
VERSION     DAOSLL010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10192)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10192)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10192
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6406_length_10192_cov_4.212489"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            147..220
                     /locus_tag="PLB25_05480"
     tRNA            147..220
                     /locus_tag="PLB25_05480"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:179..181,aa:Gly,seq:ccc)
     gene            complement(461..1756)
                     /locus_tag="PLB25_05485"
     CDS             complement(461..1756)
                     /locus_tag="PLB25_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HOE41066.1"
                     /translation="MSPSAAVLNSALSPVSAVQRVFDWHDHIALWFSLGVGLLVMQVG
                     AYLVPAVGSQDAALAIVLGSLTGAGLLAWTARLGCTSGLSSAGLMHATFGSSFARLPV
                     LLNMAQLIGWTTFELVIMRDGTAAIGKQSFGMSLDSAGGMLATTLLWGGVLLLLLGGS
                     MTQLVRKLISRIGLPLVIASLLWLTWQFGGQLQEQGLAKFWARTGDGSMGLLSAMDLV
                     IAMPVSWLPLVADYARHGKSGRSAMSGTWLGYALANIWCYGLGVLVVSVAQPGTDLVT
                     ALLLAQGGLLALSLILIDEIDNAYGDVYSGAVSGHSVKPNWSIRQWGLGLAGLCTALA
                     LVLPMHSLEPFLLMLSSVFVPLYGVILGRLSGAHVAIDPSSPKVDWSAALLWLLGVAC
                     YHLLARLAPQWGSALPTLAFTFSLAWLTRRAAVRALAQA"
     regulatory      complement(1884..1992)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2265..2795
                     /locus_tag="PLB25_05490"
     CDS             2265..2795
                     /locus_tag="PLB25_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE41067.1"
                     /translation="MSDSLEIEVRPATQRDAKAIAEVQASASQAAFKALFPGEPMPEF
                     DSLKKSQAFWREAIEYSEPQVQVAVAAAKVVGFVGYDRSRDPKTPPTTGEIWAIYALP
                     EHWGRGIGLALWDAAREGLVEEGCSKVTAWVPLGYERTLRFHDLAGFKREMTSIKTVH
                     MGAVKVEEIRFKRDLS"
     gene            complement(2830..3948)
                     /locus_tag="PLB25_05495"
     CDS             complement(2830..3948)
                     /locus_tag="PLB25_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOE41068.1"
                     /translation="MHRVCISRTGLYVPPHVISNAELVETFNQFADLENARLAADIAN
                     GSVSAIAHSSVEFIDKASGIQQRYVMEKSGVLDPRRMRPRLTARPDSELSLMAEISVA
                     AAQDALARAGKKPAAVDGVICAAANMQRAYPAMAVEIQAALGITGFGFDMNVACSSAT
                     FGIEMAVNAVKTGSARAVLVVNPEITSPHLAWKDRDCHFIFGDVCTAILVERLEGAAD
                     GAFEVLSTKLVTKFSNNIRNNAGFMSRSEDRNPDDRDQLFYQEGRKVFKEVCPMAAEH
                     IGRHLATLDLIPSDVKRFWLHQANLAMNQLIGRKLLGRDATADEAPVILNEFANTASA
                     GSIISFHRHHDDIKAGELGVICSFGAGYSIGSAVLRRA"
     gene            4095..5018
                     /locus_tag="PLB25_05500"
     CDS             4095..5018
                     /locus_tag="PLB25_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HOE41069.1"
                     /translation="MASSLLVLLDDIATVLDDVAVLTKVAATKTAGVLGDDLALNAQQ
                     VAGVKADRELPVVWAVCKGSLVNKLILVPGALLLSALAPWSITPLLMAGGLFLCFEGF
                     EKVAHKLLHSKAEDAAGHAQLLDALSDTSVDVVALEKEKIKGAVRTDFILSTEIIVIT
                     LGTVAASPFLTRLSVLAGVAVLMTVGVYGLVAGIVKIDDLGLYLSQRKSKVAQGLQQA
                     LGRMLVQAAPILMKSLTVLGTAAMFLVGGGILAHGWHAVGQGVEQISAASGALLQPLV
                     NLGLTMLFGLVAGGLAVLGITLMQRLKAKIL"
     gene            complement(5288..5372)
                     /locus_tag="PLB25_05505"
     tRNA            complement(5288..5372)
                     /locus_tag="PLB25_05505"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5336..5338),aa:Leu,seq:caa)
     gene            5411..6313
                     /gene="cysM"
                     /locus_tag="PLB25_05510"
     CDS             5411..6313
                     /gene="cysM"
                     /locus_tag="PLB25_05510"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase CysM"
                     /protein_id="HOE41070.1"
                     /translation="MKYPTIEDLIGKTPLVRLQRIAGTACQQHGNVILGKLEGNNPAG
                     SVKDRAAFSMIKRAQERGDIKPGDTLIEATSGNTGIALAMAAAVKGYRMILIMPEDLS
                     IERAQTMRSFGAELILTPKSGGMEYSRDLADQMQRDGKGLVLDQFANMDNPRIHVETT
                     GPEIWADTKGKITHFVSAMGTTGTITGVSRFLKQKNPAIKIIGVQPNEGSRIPGIRKW
                     PQAYLPKIYDPTHVDELMYVSQEDAEDMCRKMARDEGIFAGISAAGACWVAQEIAQRE
                     RHATIVFVVCDRGDRYLSTGVFPA"
     gene            6348..6575
                     /locus_tag="PLB25_05515"
     CDS             6348..6575
                     /locus_tag="PLB25_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HOE41071.1"
                     /translation="MQTDKEVDASGLNCPLPILKAKRALSELQSGQTLKVIATDAGSI
                     RDFQAFAKQTGNELVEQQSVDGSFIHVLRRR"
     repeat_region   6972..7499
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=7254..7290
                     /rpt_unit_seq="gtcttaatccctttgaattcagggcagttactttgtc"
     gene            complement(7939..8250)
                     /gene="cas2"
                     /locus_tag="PLB25_05520"
     CDS             complement(7939..8250)
                     /gene="cas2"
                     /locus_tag="PLB25_05520"
                     /inference="COORDINATES: protein motif:HMM:NF021356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HOE41072.1"
                     /translation="MSQTATARYLVTYDIADPKRLSRLFRFMKKQGVPVQYSVFLVET
                     SAAKLADLMLRIAKMIHPSADDVRAYRLPDNGWQVTMGASILPDDILPGGLMLPKRQQ
                     R"
     gene            complement(8247..>8925)
                     /locus_tag="PLB25_05525"
     CDS             complement(8247..>8925)
                     /locus_tag="PLB25_05525"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOE41073.1"
                     /translation="VVTNARAQVMRYPVARVARVVSSADVVDWSGPALALCMRAGIGI
                     TWLDSGGQAIGSIYAQKRNTADLSTVLELWSERFDGLQLYQNWLRARRMDIVVRWGEA
                     QNPSITPQQWEATKREYVYGGKFTVHLPTALRDLSLAYVAAQLNSLGLVPQLWGPRLE
                     HIDLDQDLCELLWAEMNLGTGALADTVDEATTATELFERWSARNAASLTLHLVHLHRT
                     ALKAMHT"
     assembly_gap    8926..8935
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(9030..10100)
                     /gene="cas1"
                     /locus_tag="PLB25_05530"
     CDS             complement(9030..10100)
                     /gene="cas1"
                     /locus_tag="PLB25_05530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
                     /protein_id="HOE41074.1"
                     /translation="MALLLLDRAQLEIKSEGDTLALYEAGQRRGTVPIKLLDRCVIHG
                     AQTRLDTGVLMKLAEAGVTTTLMSPRSPRRVALVLGTQHNDAAVRLAQAQRVMDDSAC
                     RKWALALVRAKLQRQLRTLRVLQSARPDARKALFDAQATVQAILAQLLVGHPFSTQGT
                     IKTEAGGAYSTGATGQFDPKDIALASLRGFEGAAARAYFAGLAGVFAPALNFAGRNRR
                     PPRDPVNVCLSLSYTMAHMQAVQSCISTGLDPLLGFYHRPSFGRESLASDLIEPLRPA
                     IDLWVWQLLRSETLRPDHFSEDKGACLLGKAGRGIYYAEWEKGSALWRRWLRLQCQTL
                     ARALRQHGELWLADPDEDDETC"
BASE COUNT         2050 a         2975 c         3058 g         2099 t
ORIGIN      
        1 catcggaagc tcccttgcac tcggaccgtt gcccaacccc aaccgtgaca acttgtaacc
       61 gtctgaatgt aggtcgacaa cttgacctcg tcaagaattc ggtccccccg gttcgattcg
      121 acatctccat tagaatcact gcccatgcgg gtgtagttca atggtagaac ggcagcttcc
      181 caagcttcat acgagggttc gattcccttc acccgctcca catctcttca gatggttgat
      241 ttagttgaat aaatcggcat tttgatggcc tggtgcagct tactcataca aaggtgggtt
      301 gtgcaaaatc tttttcgtct cccttcaggc atccatgtcc ttcgcattgc ggtgcctatt
      361 tcgttcaggc cggtgtttgg caaacgagaa gccgctacat catttataga cgctcttcaa
      421 ccctgcagta gtcaagtccg gttcatcgac aaatacccgg ctatgcctgc gcgagcgcac
      481 gcacagcggc ccggcgtgtc agccaggcca gcgaaaaggt gaaggccagg gtgggaaggg
      541 cagaacccca ctgtggggcc aacctggcca acaggtggta gcaggcaaca cccagcagcc
      601 agagcagcgc ggcagaccag tccaccttgg gcgaagacgg gtcaatggca acatgcgcgc
      661 cactgagccg acccaggatc accccgtaca gcggcacaaa aaccgagctt agcatcagca
      721 gaaatggctc caggctgtgc attggtagca ccaacgccag ggcggtgcaa agacccgcca
      781 gacccagtcc ccactggcga atgctccagt ttggcttgac gctatggcct gatacggcac
      841 ccgagtacac gtcgccatag gcattgtcaa tttcatcaat cagaatcaga cttagcgcca
      901 gcaggccacc ttgcgcgagc agcagcgccg tgaccagatc ggtgccaggc tgtgccacac
      961 tcaccaccaa cacacccagg ccatagcacc agatgttggc cagcgcatac cccagccagg
     1021 tgccgctcat ggcgctgcgt ccggatttgc cgtggcgggc atagtcggcc accaatggca
     1081 gccacgacac cggcatggcg attaccaggt ccattgccga caaaagtccc atgctgccat
     1141 cacctgtgcg cgcccagaac ttcgccaggc cctgctcctg caattggccg ccaaactgcc
     1201 aggtcagcca aagcagcgag gcgatcacca gcggcaggcc aatgcggctg atcagcttgc
     1261 gtaccagttg ggtcattgac ccgcccagca gcagcagcag cacaccaccc cacaacagtg
     1321 tggtggccag catgccgcca gcgctgtcca gggacatccc aaaagactgt ttgccaatgg
     1381 cggccgtacc gtcgcgcatg atgaccagct caaaggtggt ccagccgatc agttgcgcca
     1441 tgttcagcag caccggcagc cgggcaaagc tgctgccgaa agtggcatgc atcaaaccgg
     1501 cgctggacaa gccgctggtg cagcccagcc gtgccgtcca ggccagcaaa ccagcaccag
     1561 tcagcgaacc cagcactatc gccagcgctg cgtcctggct gcccacggct ggcaccagat
     1621 aggcacctac ctgcatcacc agcaggccca cgcccaggct gaaccacagc gcgatgtggt
     1681 catgccagtc aaagacccgc tgcaccgctg ataccggtga cagtgccgaa ttcaatactg
     1741 ccgctgatgg cgacattggc atcgaaggag agttatgtgt cccggaaatg ggtgtactgg
     1801 ccatgcgttt gctccatgca aaaaactggc aggtcggcga aatccaaata ggggatgtga
     1861 gttgccagag aaggcggcaa cacccgttgt cagactggct tccctgcgcg aggattacct
     1921 caatcaggtt caaagggact ttctcagccg ctttgcagat tcaactgcag agcaacaccc
     1981 ctagcgtgtg tgtcaaataa tcaacaccgg gcggattcta attccatttc caaatcattc
     2041 gtcccttggc acccggtcgc agaccttgcc aacgccaggc aaggcgcgat cgcaaggact
     2101 ggactggttc agcaaaagga ataccgcaaa aagaagatgc aaaagtgcct gctttgcatc
     2161 aatcagcccc tcaagagtcg cacctggccg accgggacgt caatgcagcg acaattcatc
     2221 cccctgtttg ttgacccgcc ggacaagacc ccggccattt tttcatgtct gattcattag
     2281 agattgaagt ccgtcccgcc acccagcgcg atgccaaagc aatagccgaa gttcaggcca
     2341 gtgccagcca ggccgccttc aaggcgcttt ttcctggcga gccaatgccc gaatttgaca
     2401 gccttaagaa aagccaggca ttttggcgcg aagccattga gtacagcgaa ccccaggtgc
     2461 aggtggctgt cgctgccgcc aaggtggtcg gatttgtcgg ctatgaccgc tcgcgtgacc
     2521 ccaaaacacc acctaccacc ggcgaaatct gggccatcta tgcgctgccc gagcattggg
     2581 gccggggtat tgggttggcg ctttgggatg ccgcgcgtga aggcctggtt gaagaaggtt
     2641 gcagcaaagt caccgcctgg gtgccgctgg gttacgagcg gacgctgcgc tttcacgacc
     2701 tggcgggttt caagcgcgag atgaccagca tcaagaccgt gcacatgggc gcggtcaaag
     2761 tggaagaaat tcgcttcaaa cgcgatctga gttgaagtgc aagccctgcc gggccaaaga
     2821 tggcgtgctt caggcgcggc gcagcaccgc actgccaatc gaatagccag ccccaaacga
     2881 gcaaatcacg ccaagttcgc ccgccttgat gtcgtcatgg tgacgatgga acgaaatgat
     2941 cgagccggct gaagcggtgt tggcaaattc attgagaatc acgggcgctt cgtctgccgt
     3001 ggcatcgcgc ccgagcagtt tgcggccgat caactggttc atggccaggt tggcctggtg
     3061 cagccagaag cgtttgacat cactggggat caaatccagc gtagccagat gcctgccgat
     3121 gtgttcggcc gccatcgggc agacttcctt gaacaccttg cggccttcct ggtaaaacaa
     3181 ctggtcgcgg tcatccgggt tgcggtcttc gctgcgcgac atgaagccgg cgttgttgcg
     3241 gatgttgttg gaaaacttgg tgaccagttt ggtgcttagc acttcaaagg caccgtctgc
     3301 tgcaccttca agccgctcca ccagaatggc ggtacagaca tcgccaaaaa tgaaatgaca
     3361 gtcgcggtct ttccaggcca ggtgcggcga ggtgatttca gggttgacca ccagcaccgc
     3421 gcgcgcgctg ccagtcttga ccgcgttgac cgccatctcg atgccaaaag tggccgacga
     3481 gcaggccacg ttcatgtcaa aaccaaagcc tgtaatgccc agtgccgcct gaatttcaac
     3541 tgccatggcc gggtacgcgc gctgcatgtt ggcggcggcg catatcacgc cgtccaccgc
     3601 cgcgggcttc ttgccagcgc gggccagggc atcttgcgct gcggccacgc tgatttctgc
     3661 catcagcgac agttcgctgt ccgggcgcgc tgtcaggcgc gggcgcatcc gcctggggtc
     3721 aagcacgcca gacttttcca tcacatagcg ctgttgaatg cccgatgcct tgtcaatgaa
     3781 ttcgacgctg gagtgcgcaa tggcgctcac gctaccgttg gcaatgtcag ccgccagtct
     3841 ggcgttttcc aggtcggcaa actggttgaa tgtttccacc agctcggcat tgctgatgac
     3901 atgggggggc acatatagcc cggtgcggct gatacaaacg cgatgcatga cggctctttc
     3961 tgaattcggg acaatccccg gtaggtgttt tcgggccaca ttatcgaacc tgccccggcc
     4021 ccaaaccggt ggcgggcttc aacgacaatc ggaaaaaagt gattcgattg ttttcaacat
     4081 aggagagttg tctgatggcc agtagcttgc ttgttttgct cgacgacatt gccaccgtgc
     4141 ttgacgatgt ggcggttttg accaaggttg cggcgaccaa gaccgccggg gtgctgggcg
     4201 acgatctggc gctgaacgcg caacaggtgg ccggggtcaa ggcagaccgg gaactgccgg
     4261 tggtctgggc cgtgtgcaag ggttcgctgg tcaataaact gattttggtg ccgggtgcgc
     4321 tgctgctcag cgcgctggca ccctggtcga tcaccccctt gttgatggcc ggtggcctgt
     4381 ttctgtgttt cgagggtttc gagaaggtgg cgcacaagct gctgcacagc aaggccgaag
     4441 acgcagccgg gcatgcgcaa ctgctcgatg ccctgagcga cacgtcggtg gatgtggtgg
     4501 cactcgaaaa agaaaaaatc aaaggcgctg tacgcaccga cttcattttg tccaccgaga
     4561 tcatcgtcat caccctgggc acggtagcgg ccagcccgtt tctgacccgg ctgagcgtgc
     4621 tggccggtgt ggcggtgctg atgacggtcg gggtctatgg gctggtggcg ggcattgtca
     4681 agattgacga cctgggcttg tacctgagcc agcgcaaatc gaaagtggcg caagggctgc
     4741 aacaggccct aggccgaatg ctggtgcagg ccgcaccgat cctgatgaaa agcctgaccg
     4801 tgctgggcac ggccgccatg tttctggtgg gcggcggcat tctggcgcat ggctggcacg
     4861 cggtagggca gggtgtcgag cagatcagcg ccgccagcgg cgcgctgctg caaccgctgg
     4921 tcaacctggg cctgaccatg ctgttcggcc tggtggccgg tggtctggcg gtgttgggca
     4981 tcacactgat gcaacgcctg aaagccaaaa tcttgtaaga ctttttggca tccagccctt
     5041 atcgagaaag cgctgatagc tatcataatt gaagctattt cagcttgaat tctggccgcc
     5101 atgccagttg gccgacttgt ggcaggaggt gccccggtgg cccagtttct gcatgttgtg
     5161 accccgatga aacacaagcc cgcagcccgg ccaacgtgca acgcgcgtta ccgagcgaca
     5221 gcagcagagt tttcacaggg ggaaacaaaa aaggccggac agcgcaagaa cgtgtcggcc
     5281 ttatcgatgg tgcccggggt cggaatcgaa ccgacacacc tttcggtact ggattttgag
     5341 tccagcgcgt ctaccaattc caccacccgg gctttgatca gagagccgga attatggcac
     5401 agtgtgggct atgaaatatc caaccattga agatttgatc ggtaaaaccc cgctggttcg
     5461 cctgcaaaga attgccggca cagcatgtca gcagcatggc aatgtgattc tgggcaagct
     5521 cgaaggcaat aacccggcgg ggtcggtcaa agaccgggcc gcgttttcga tgatcaagcg
     5581 ggcgcaagag cgcggcgaca tcaagcccgg cgacacgctg atcgaagcca cctcgggcaa
     5641 caccggcatc gcgctggcca tggcagcggc agtcaagggc taccgcatga ttctgatcat
     5701 gcctgaagac ctgtcgatcg agcgggccca gaccatgcgc tcctttggtg ccgagctgat
     5761 cctgaccccc aagagcggcg gcatggagta ttcgcgcgac ctggctgacc agatgcagcg
     5821 cgacggcaag gggctggtgc ttgaccagtt tgccaatatg gacaacccgc gcatacacgt
     5881 tgaaaccacc ggccctgaaa tctgggccga caccaagggc aagatcaccc actttgtcag
     5941 tgccatgggc accaccggca ccatcaccgg ggtttcgagg ttcctcaagc aaaaaaatcc
     6001 ggccatcaaa atcattggcg tgcaacccaa tgagggctcg cgcattccgg gcatccgcaa
     6061 gtggccgcag gcctatttgc ccaagatata tgacccgacc catgtcgatg aattgatgta
     6121 cgtgagccag gaagatgccg aggacatgtg ccgcaagatg gcgcgcgacg aaggcatatt
     6181 tgctggcatt tcggccgccg gggcctgctg ggtggcgcaa gaaattgccc agcgcgagcg
     6241 acacgcgaca attgtctttg tggtgtgtga tcgcggcgac cgttatctgt ccaccggcgt
     6301 gttcccggcc tgaagctttg gccatcaaat actttttgga aaacctgatg caaaccgaca
     6361 aagaagttga tgccagtggc ctgaactgcc cgctgcccat tttgaaagcc aaaagggcgc
     6421 tgagcgaatt gcaaagcggc cagacgctga aggtgattgc caccgatgcc ggttcaatcc
     6481 gcgactttca ggcttttgcc aaacagaccg gcaatgaact ggtggagcag caatccgtgg
     6541 acggcagctt catccatgtg ttgcgccgcc gctgaacctt gcctaaaggc ctggcaataa
     6601 agtcgcttga ctgacctgcc gattcgggca tgcgcagcaa ggcatcagcc ccgcttttgg
     6661 ggctgcaagc cagccgggca gaactgaaaa cccatggcag tgtggtcaca agctgatgca
     6721 gcatttgcgc agccatcagc ctcgggcggc gatgtcttgc cagttttggc gcgtatttca
     6781 gcgatagtga acccgtcttt tggtgtgatt gcacacggca tttatagctg gtagtggatg
     6841 tggtcgtggt cgtgccgtgc gcgtgccagc aacgcatgcc tttggcacaa atcccgcaaa
     6901 caagcattgc cgggaaccgc cttgatgcgg ggtttggcgt tctggcttgc ggggttgaga
     6961 cttcttatca gactctcgcc tgggtcatcg gcaggcagtt actttgtcca tcggcggcat
     7021 gatgaccctg gacgcggccc cgccttaatc cctttgaatt cagggcagtt actttgtctt
     7081 ttgagtgata agcgagccag ccgcaccgct ggttttaatc cctttgaatt cagggcagtt
     7141 actttgtcgc ggcctgttgt agcgcatcaa cttgttacgg ggtcttaatg cctttgaatt
     7201 cagggcagtt actttgtcta catggcggtc aaggcattta agttcatccg tgcgtcttaa
     7261 tccctttgaa ttcagggcag ttactttgtc agatacgtga gcaatgccaa caactcgcat
     7321 gtgtcttaat ccctttgaat tcagggcagt tactttgtcg gccaattttt tgggtatcgc
     7381 agagtccacg ctgtctttat ccctttgaat tcagggcagt tactttgtct ggcaacaata
     7441 caattgtcag ccgctattat tagtcttaat ccctttgaat tcagggcagt tactttgtct
     7501 gtattggtca aaaaacgcta cagattcaac agcttgcatc gggagttcga gacagttggg
     7561 cgtgttgaac aacctaactt ccagccaacc ttcctttccc ccgctgcttg cggccgccct
     7621 gtgcaaggct ttgagtctat cgggtctggc gcaccgcact ggtgtctttc gcagcctggt
     7681 cggtgggttt ggtttgtggt ctgcggcgtt cggggtcagc gtcaacggcg ctacagtttg
     7741 cctggcgcag aaatggcact gatggcaggc agttgcgtca cgctgttgtt tgggcatttg
     7801 attgccctgc atggcggcac ggcagcaggc attcgtgctg gcatgcgcct tgggcatttg
     7861 cattgctatc atagttgtat ctgctcgcgc atgctgcata agggctggaa ggcctttttg
     7921 cattgaacgc cgaccgggtc agcgctgttg ccgctttggc agcatcagtc cgccgggcag
     7981 gatgtcgtcg ggcaggatgc tggcgcccat cgtcacctgc cagccgttgt cgggcaggcg
     8041 gtaggcgcgc acgtcgtcgg cgctggggtg gatcattttg gcaattctca gcatcaagtc
     8101 ggccagtttg gcagcgctgg tttccaccag aaacaccgaa tattgcaccg gcacaccctg
     8161 ctttttcatg aacctgaaca ggcggctcag gcgcttcggg tcggcgatgt cgtaggtgac
     8221 caggtagcgg gcagtggcag tctggctcat gtgtgcatgg ctttcagggc ggtgcggtgc
     8281 aagtgcacca ggtgcagggt caggctggcg gcattgcgtg cgctccagcg ctcgaacagt
     8341 tcggtggcgg tggtcgcctc atccactgtg tcggccagtg cgccggtgcc aagattcatt
     8401 tcggcccata gcagctcgca caggtcttgg tccaggtcga tgtgttccag cctcggtccc
     8461 caaagctggg gcaccaggcc aaggctgttg agctgggccg ccacataggc caggctcagg
     8521 tcgcgcaggg cggtgggcag gtgcacggtg aatttgccgc catacacata ttcgcgcttg
     8581 gtggcttccc attgctgcgg ggtgatgctg gggttttgcg cctcgcccca gcgcaccaca
     8641 atgtccatgc gccgcgcgcg cagccagttc tggtaaagct gcaggccgtc aaagcgttcg
     8701 ctccacagtt ccagcacggt cgacaggtcg gcggtgttgc gcttttgcgc gtagatgctg
     8761 ccaattgcct ggccaccgct gtcaagccag gtgatgccga tgcccgcgcg catgcacagc
     8821 gccagcgccg ggccgctcca gtccaccacg tcggcgctgc tgaccacccg cgccacacgg
     8881 gccaccgggt agcgcatgac ctgggcgcgg gcgttggtga ccaccnnnnn nnnnntcagt
     8941 gcagctcacg cgcttgtgct cgcggctgcc caggtacagg gcgcgggcac cggggccggg
     9001 ctggatgcgc cgggtttggc ttttgtcggt cagcatgttt cgtcatcctc atcggggtcg
     9061 gccagccaca gctcgccatg ctggcgcagc gcccgcgcca gcgtctggca ttgcagacgc
     9121 agccaacgcc gccacagggc gctgcctttt tcccattcgg cgtaatagat accacgcccg
     9181 gccttgccca gcaggcaggc gcctttgtct tcgctgaagt ggtcggggcg caaagtttca
     9241 ctgcgcagca gttgccatac ccagaggtcg atggccgggc gcagtggctc gatcaggtcg
     9301 ctggccaggc tttcgcggcc aaagctgggg cggtgataaa agcccagcag cgggtccagc
     9361 ccggtgctga tgcagctttg caccgcctgc atgtgcgcca tggtgtagct caaagacagg
     9421 cagacgttga ccgggtcacg cggcgggcga cggttgcgcc cggcaaagtt cagcgctggc
     9481 gcaaacaccc cagctaggcc ggcaaaatag gcgcgtgcgg cagcgccttc aaaaccgcgc
     9541 aggctggcca gggctatatc ttttggatca aattggcctg tagcgcccgt ggaatatgcg
     9601 ccaccagctt ctgtttttat agtgccctgg gtgctgaaag ggtggccgac cagcagttgg
     9661 gccaggattg cctgcaccgt ggcttgcgcg tcaaacagcg ccttgcgggc gtcggggcgg
     9721 gcgctttgca gcacacgcag cgtgcgcaac tggcgttgca gcttggcacg caccagtgcc
     9781 agcgcccatt tgcggcaggc gctgtcatcc atcacgcgct gggcttgcgc caggcgcact
     9841 gccgcatcgt tgtgctgcgt gcccagcacc agcgctaccc ggcgcgggct gcgcgggctc
     9901 atcagggtgg tggtgacacc ggcttcggcc agtttcatca acacgccggt gtcaagccgg
     9961 gtttgggcgc catggatcac gcagcggtcc agcagtttga tgggcaccgt gccacggcgc
    10021 tggccagcct cgtacagggc cagggtgtcg ccttcggatt tgatttcgag ctgggcgcgg
    10081 tcaagtagca gcagggccat ggtgatcttt cggttaatgg tgttttaggc actgatcaag
    10141 tctttgcaat ttccgggaag aattcaagaa agtcatggag tcatggagtc at
//
LOCUS       DAOSLL010000087        10176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6415_length_10176_cov_3.980239, whole genome shotgun sequence.
ACCESSION   DAOSLL010000087 DAOSLL010000000
VERSION     DAOSLL010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10176
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6415_length_10176_cov_3.980239"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /gene="cmr6"
                     /locus_tag="PLB25_05535"
     CDS             complement(<1..877)
                     /gene="cmr6"
                     /locus_tag="PLB25_05535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr6"
                     /protein_id="HOE41075.1"
                     /translation="MIPDLVAALPDRIPAQFGDAAPGHRFHLYFTHWEAGRFSPYKDA
                     KLPDIKHLEKTEREKIRTEFEARKRRALGNVCPMGDSAKAQLVAVRARQKALFNHYGP
                     LGYRLSTTSTAPFATGLGNEHPIENGFAFLTPYGLPYLAGSGVKGILRRSMQELQNEG
                     EVGFTDEAINALFGPELIAKPEDARRGALDFWDVFPDPAKVGGKLVVEIITPHFGGYY
                     QKNETPHDSGSPNPVAFLAVPAKSQFDFHVVCQPDRLPAALQNQWRVLLQRAFQHAFE
                     WVGFGAKTSVGYGAMA"
     gene            complement(881..1273)
                     /gene="cmr5"
                     /locus_tag="PLB25_05540"
     CDS             complement(881..1273)
                     /gene="cmr5"
                     /locus_tag="PLB25_05540"
                     /inference="COORDINATES: protein motif:HMM:NF021237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated protein
                     Cmr5"
                     /protein_id="HOE41076.1"
                     /translation="MTQTLDQQRAALAWQYATNSRKLQGELPDKYRNLAKGAPALIMN
                     SGLMPTLAFYSSKSKAREEVAESALLKDLIAGMIQRLAPQAAPRDFASFMTMLQKCES
                     RDYLRFTDEALELLKWLRQFVDAVKSKD"
     gene            complement(1257..1712)
                     /locus_tag="PLB25_05545"
     CDS             complement(1257..1712)
                     /locus_tag="PLB25_05545"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOE41077.1"
                     /translation="MTVGATDMLQVYLDANATIYFVERVEPYFTRLKTLMLDHQGRPR
                     INCCINELLLMEVRVKPLREDDQVLLLRFEHYFQIFASQFVALDKSVFDLATQIRVKH
                     GSKTPDALHLAAAIHAGCDQFWTHDGKLVKAAQGRIEVINIGKPHDTDT"
     gene            complement(1693..1950)
                     /locus_tag="PLB25_05550"
     CDS             complement(1693..1950)
                     /locus_tag="PLB25_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41078.1"
                     /translation="MQAYRFDRNVAQSHTITLELPPEAPTGPAQIIVLFPETPRTSAD
                     NSPRFADIAEYIAWLETQPPTGRSREEIDRQVQDEHDSWGD"
     gene            complement(1968..2951)
                     /gene="cmr4"
                     /locus_tag="PLB25_05555"
     CDS             complement(1968..2951)
                     /gene="cmr4"
                     /locus_tag="PLB25_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module RAMP protein Cmr4"
                     /protein_id="HOE41079.1"
                     /translation="MFEASQLVFYYAISPVHMGAGSAIGAIDSPIQREVHTQHPMFAG
                     SGLKGALRHHFNRAWVRGEGDSSKPNSLINRIFGPDTGASDFAGALSLSDAQLLALPV
                     RSPKGGFAYVTSPLALARLQRLAQQAGLPCDWAVPSVFENRALAASNALLTQRPGTPA
                     GAVADQLVLEAFEFTASVEHLQAIAKWIADYALPSSANAFFANKFKTDLVLLNDTDFA
                     HFARHAMVVEPHVRINDESGTADDGGLFYVENLPPETLMVGLAQASIERFKKGSRSVD
                     AAALLNAPEILAKVFVGQGETLPGIGGKLLQIGGDASTGRGLVLVQAIHQN"
     gene            complement(2992..4176)
                     /locus_tag="PLB25_05560"
     CDS             complement(2992..4176)
                     /locus_tag="PLB25_05560"
                     /inference="COORDINATES: protein motif:HMM:NF021236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR module-associated Cmr3 family
                     protein"
                     /protein_id="HOE41080.1"
                     /translation="MNATTYFYVRPTDSLFVRGNLAFGDSGEHGTGLMPPPPSLFAGA
                     FRSALLGQDVKHLSSFLSQGRCSDAALEQCLGTPRMPGEFRITWLSLAGEPSAGALPE
                     PVFPLPADLLMLGMTFATLEPRAVAAGVQSAGDLPLRATLVSAKQEKPNGGTLLSARG
                     LHQHLSGQRPEQAHGIETKHLYQRDPRLGIGLNTDAHTAEEGQIYTTEGFAFSPKSAG
                     MPAKFASTGFLVGIDGVAELMPAEGVLRLGGDGRSALYRRVNFTPPAEPGLPGKSRRF
                     RLLLQTPALFSQGWLPEGVVRQNDDSYHLQGDGFDARLACTALGRRDTVSGWDLFQWA
                     PKPAQAAVPAGSVYWFDEFDGDFGKLAAWVSQGLWPQNPDTLQHSRRAEGYNRALLGA
                     WN"
     gene            complement(4173..7202)
                     /gene="cas10"
                     /locus_tag="PLB25_05565"
     CDS             complement(4173..7202)
                     /gene="cas10"
                     /locus_tag="PLB25_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III-B CRISPR-associated protein Cas10/Cmr2"
                     /protein_id="HOE41081.1"
                     /translation="MTHNTNYTKKLAAWLHDPAEKQLVLMRDAEGHEGGTARRLRQAL
                     DISAKEFDKRADWLAAAADRPSWPRNDDARRYPQFEAVKFTKEAELIHPLSGEKIELK
                     KLDLGINVEQIKAVSQAHFESLIQHDDDSLRKTFLAFWRFGPEAGQHASELGVLWPML
                     PADSRVPDHSIWSHIDTVSAIHTALAGDDTGQDQPALLVMSFGPVQGFIGQARSTSDL
                     WAGSHLLSCLVFEAMKPIISHLGPDCMVFPALRGVPLVDAWLMSPEVGGDAFVKLFDD
                     IDSELLADRTDTNPLFAASLPNKFMAIVPSKQAAALAERALEAIRNAAKQWTTEAAKR
                     AFDAAGVALTDVTRAQIKTQLAGFPEAYWAAATWPIDGDGCRDLAAASARLHAALDSI
                     HPDLKKQGVFQEATWQVLTKELKLDGLQFWQPNTGILYPAVYELAERALGAAKATRPF
                     APVLEEGHRCTLTGDGEWLTHDGALLGLNRKDRMADSVWGKLAKKKKSWVKPGEHLSA
                     IATLKRLWPTLFAERVSRLTGGDVRRFVVSTHALALSTTLEKLTDPNQPLTTDQTSAL
                     QVLAASAKDFDAVTLPKALVRTLYNREQLSQTELDTLRRLPTMLEDADDGSAESNHRS
                     RTIARLLGDNRPEAYYALILMDGDRMGGWLAGNEDDYKLSYRQTWHSKVLGEMAKFEG
                     QDKALKVYLDTKRPVSPGRHGAISQALNDFSTRLARHVVEECCKGKLLYAGGDDVLAL
                     VAVDDLFDCMQLLRLAYSGIAPDDSMGLGKHLGQLEKGGSDAQRKLLLKSGFGLLPGQ
                     HLGNGRFSASRLMTLMGHKATASMGAVVAHHSAPLGMVLRELRAAESRAKNTVRGKNA
                     RGDDLNRDAFCLRVLKRGGGEVSVTSPWWPVNVANQQPDPSQSALSLMKELAQQLALT
                     DFSRGAIYRAQLWFEGLTDDAGDAKTPAWRDQMTSSLAHQFSRQKGDPAVARQVVNFV
                     CDVMQAQHPRTAIDSFLVTSEFFARETRAFAPTGAAA"
     gene            complement(7207..8340)
                     /locus_tag="PLB25_05570"
     CDS             complement(7207..8340)
                     /locus_tag="PLB25_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41082.1"
                     /translation="MTMRELSYQLSFNTPAFLGNAEQQAQWRTPPFKALLRQWWRVVK
                     AADLGYDHREMLRLENSLFGSAGDDAGGGRSKVQLRLSTWDTGTLTSLPHMATQQHSE
                     VKNRQTGQLMSVGTDVYLGFGPVTTHGNRNAIAPEGPPMTFKLRCPDAEAGTLRKAMQ
                     LAAWFGSVGSRARNGWGSLHIEGEGLLGFEGLCDSGLALQSAPRSLSAALTDRLAGEW
                     PHALGLCTDGRPAVWRVFADKKLNAEGKLIFTGFKEWKSVLERLAALKIGFRTRFKLS
                     SGSPHNRVEARHVLAYPVTNHGLAGLPNARLASQMRFKVAKNRDGQYFGLITHLPSAM
                     PKAFFDRSQVTQLPINDQIEVWNQVHAFLNARPASEITRIRKG"
     gene            complement(8337..9323)
                     /gene="csm6"
                     /locus_tag="PLB25_05575"
     CDS             complement(8337..9323)
                     /gene="csm6"
                     /locus_tag="PLB25_05575"
                     /inference="COORDINATES: protein motif:HMM:NF021162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated ring nuclease Csm6"
                     /protein_id="HOE41083.1"
                     /translation="MRAELSLLSDDLGWFHRLCADYHLPGVKFDRSHIHVMRDASGQP
                     MNDIRTPADNRAAADFITAHVRAITADSGCALHASIAGGRKTMGFYLGYALSLFGRPQ
                     DRLSHVLVSEPFESSYDFFYPTPYSRVLQTRDGQLADTAMAQVTLAEIPFVSLRHGLP
                     TSLLAGVASFNDTVAAARAALAPAQLVLDLARQCILAGRREIALPPAELALLAVFARE
                     TLAGNQPLSAPTKGVPDPLWATRYLREYRRITGNLADIEGTERALRNGMDGEYFSARK
                     SKLDKRLKTALGPAAQAYRIHDGDVRPRRYGLTLHPDAVRFAADVVLKEENK"
     gene            complement(9295..9507)
                     /locus_tag="PLB25_05580"
     CDS             complement(9295..9507)
                     /locus_tag="PLB25_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41084.1"
                     /translation="MKPIDVPHPVALTQSADFPRRLLLAVSGLTPQIVTETLYALAAD
                     EFAPFVPTEVHLITSAEGARRAVLAQ"
     gene            complement(9710..10063)
                     /locus_tag="PLB25_05585"
     CDS             complement(9710..10063)
                     /locus_tag="PLB25_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41085.1"
                     /translation="MNKQFNRRGWFGGVLALCLGFSGAAMAQSIRERVRIAEARIDQG
                     IRSGALTRPEANRLIMELRQVRDDELRARRNGRIDPYERERLDRELDRLEAHITDLKH
                     NYNSRDDLPPRRYWR"
BASE COUNT         1935 a         2935 c         3161 g         2145 t
ORIGIN      
        1 ctgccattgc gccgtaaccc actgaggttt tggcaccgaa accgacccat tcaaaggcgt
       61 gttgaaaggc ccgttgcagc aaaacacgcc attggttttg cagtgccgcc ggcaaacggt
      121 cgggctggca gaccacgtgg aagtcaaatt gcgatttggc gggcacggcg agaaaggcga
      181 ccggattggg tgaaccgctg tcgtgcgggg tttcgttttt ttggtagtaa ccgccaaagt
      241 gtggggttat gatttcaacc accagtttgc cgcccacctt ggccggatcg ggaaatacgt
      301 cccagaagtc gagggcacca cgacgtgcat cttcgggctt ggctatgagt tccgggccaa
      361 acaaggcgtt gatcgcctcg tcggtgaaac cgacttcacc ttcgttctgt agctcctgca
      421 tggaccggcg cagaatgcct ttaacaccgc tgccagccag atacggcaag ccgtaggggg
      481 tgaggaaggc aaagccattt tcaatcgggt gctcgttgcc cagaccggtg gcgaaggggg
      541 ctgtggaagt ggtgctgaga cgataaccga gggggccgta gtgattgaac aatgcttttt
      601 gacgcgcacg cacggcgaca agttgggctt tggcggaatc ccccataggg caaacgtttc
      661 ccagcgccct ccttttgcgc gcttcaaatt ccgttcgaat tttctcccgc tctgtttttt
      721 ccagatgctt gatgtctggc agtttggcat ctttgtatgg cgaaaaacgg cctgcctccc
      781 aatgtgtaaa gtaaaggtga aatctgtgac cgggcgcggc atcgccaaat tgcgccggaa
      841 tgcgatcagg tagggcagcg acaaggtcgg gaatcatgga ctaatccttc gatttaacgg
      901 catctacaaa ttgccgcagc cacttgagca actcaagtgc ctcgtcggta aagcgcagat
      961 agtcgcggga ttcgcacttt tgcagcatgg tcataaacga tgcgaagtca cgcggtgctg
     1021 cttggggtgc caagcgttgg atcattccgg caatcaaatc tttcagcagc gcggattccg
     1081 cgacttcttc ccgggctttg gatttgctgc tgtagaaagc cagtgttggc atcaggccac
     1141 tgttcatgat gagcgctggc gctcctttgg ccaaattgcg gtatttgtcg ggcaattcgc
     1201 cttgcagttt gcggctgttg gtggcgtatt gccaggccag cgcggcgcgt tgttgatcaa
     1261 gtgtctgtgt catgtggctt ccctatgttg atgacttcaa ttcgcccttg cgccgctttc
     1321 acgagcttgc cgtcgtgcgt ccagaactga tcgcatcccg cgtgaatggc tgcggcgagg
     1381 tgcagtgcat ccggggtttt ggacccgtgt ttgactcgaa tttgggtggc cagatcaaat
     1441 accgatttgt caagggcaac gaattggctg gcaaatattt gaaagtagtg ctcaaagcgc
     1501 aacagcagca cttgatcgtc ctcccgaagc ggcttgacgc gcacttccat cagcagcaat
     1561 tcgttgatgc agcaattgat gcgtggcctg ccctgatggt ccagcatcag cgttttcagg
     1621 cgggtgaaat acggctcaac ccgctcgacg aagtaaatcg tggcattggc atccagatac
     1681 acctgcagca tgtcagtcgc cccaactgtc atgctcgtcc tgcacctgcc ggtcgatttc
     1741 ttcacggctg cgaccggtgg gcggctgtgt ctcgagccag gctatgtact cggctatgtc
     1801 ggcaaagcga ggtgagttgt cggcgctggt gcgtggggtt tcggggaaca gtacgatgat
     1861 ctgggccggc ccagtcggtg cctcgggcgg tagttccagg gtgatggtgt gcgattgcgc
     1921 gacattgcgg tcgaaccgat aagcctgcat gttgtactcc ttgtgcgtca gttctgatgg
     1981 atggcctgca ccagcacaag cccacgcccg gtgctggcat cgccgccaat ttgcagcagt
     2041 ttgccaccga tgccgggaag cgtttcgcct tggccgacaa acactttcgc gagtatttct
     2101 ggagcgttca gtagcgcggc ggcatcaaca gagcggctgc ctttcttgaa gcgctcaata
     2161 ctggcttgcg ccaggccaac catcagggtc tcgggcggca ggttttccac ataaaacagg
     2221 ccgccgtcgt cagcggtgcc gctttcatcg ttgatgcgca catgcggctc caccaccatg
     2281 gcgtggcggg caaagtgggc aaagtcggtg tcgttcagca gcaccaggtc agtcttgaat
     2341 ttgttggcaa agaaggcgtt ggcgcttgat ggcaaagcat agtctgctat ccattttgct
     2401 attgcttgca ggtgctccac gcttgccgtg aattcaaatg cttctagcac cagttggtcg
     2461 gctaccgcgc ccgccggtgt gccagggcgt tgcgtcagta gggcattgct cgcggccagc
     2521 gccctgtttt caaagaccga tggcacggcc cagtcgcagg gcagcccagc ttgttgcgcc
     2581 agccgctgca aacgcgccag tgcgagcggg ctggtgacat aggcaaaacc gcctttaggc
     2641 gagcgcaccg gcaaggcaag caattgcgcg tcgctcagac tgagcgcacc ggcaaagtcc
     2701 gacgcaccgg tgtcggggcc aaaaatgcgg ttgatcaagc tgttgggttt gctgctgtca
     2761 ccctcgccgc gcacccaggc gcggttgaag tggtggcgca gcgcgccttt gaggccacta
     2821 ccggcaaaca tcggatgctg tgtgtggact tcgcgctgaa tggggctgtc aatggcccca
     2881 atggcgctgc ccgcacccat gtgaaccggg ctgatggcgt agtaaaaaac gagttgcgag
     2941 gcttcaaaca taacgaggtc cttgagaggt tacggtgtgt gacagttaaa ttcagttcca
     3001 ggcacccagc agggcgcggt tgtagccttc agcacggcga ctgtgttgca gcgtgtccgg
     3061 attttgcggc cacaagcctt ggctgaccca ggcggcaagc ttgccgaagt caccatcaaa
     3121 ctcgtcaaac caatacacgc tgccagcagg cacggcggct tgcgcgggtt tgggtgccca
     3181 ctgaaacaaa tcccaaccgc tgacggtgtc gcgccgaccc agtgctgtgc aggccagccg
     3241 ggcatcaaaa ccatcacctt gcaggtggta actgtcatcg ttctgccgta ccacgccttc
     3301 gggcagccag ccctggctga ataaggccgg ggtttgcagc aacaggcgaa agcgcctgga
     3361 tttgccgggc aggccgggct cagcgggcgg ggtgaagttg acgcggcggt aaagcgcact
     3421 gcggccgtcg ccgcccaggc gcaacacgcc ttcagcgggc atcagttccg ccacgccgtc
     3481 aatgcccacc agaaaaccgg tgctggcgaa cttggctggc atgccggcac tctttgggct
     3541 aaaggcaaag ccctcggtgg tgtaaatctg accttcttcg gcagtgtgtg catcggtgtt
     3601 gaggccaatg cccagacgcg ggtcgcgctg gtacaggtgt ttggtctcga tgccatgtgc
     3661 ctgctcgggg cgctgcccgc tcaggtgctg gtggagcccc ctggcgctca aaagggtgcc
     3721 gccgttaggt ttttcctgct tggctgacac cagtgtggcg cgcaagggca gatcaccagc
     3781 agattggaca ccagcagcga cagcgcgcgg ctccagcgtg gcaaaggtca tgcccagcat
     3841 cagcagatca gcaggaagtg ggaaaacggg ttctggtaac gcgccagcgc tgggttcacc
     3901 cgccagcgat aaccaggtga tgcggaattc gcctggcatc ctgggtgtgc ccaggcattg
     3961 ctctagcgcg gcatcactac agcgcccttg tgacagaaaa ctgctcaggt gcttgacatc
     4021 ttgccccagc aaggccgagc gaaacgcccc ggcaaacaac gagggtggcg gcggcatcaa
     4081 gccagtgcca tgttcgccgc tgtcgccaaa agccaggttg ccacgcacaa acaggctgtc
     4141 ggtagggcgc acgtagaaat aagtggtggc gttcatgcgg ccgctccagt gggcgcaaaa
     4201 gcgcgggttt cgcgggcgaa aaattcgctg gtgaccagaa agctgtcgat ggcagttctg
     4261 gggtgctggg cttgcatcac gtcacagaca aagttgacga cctgccgcgc cacagcaggg
     4321 tcacccttct ggcggctgaa ctggtgggcc agtgaactgg tcatctggtc gcgccaggct
     4381 ggggttttgg catcacccgc gtcatcggtc aaaccctcaa accacaactg ggcgcggtag
     4441 atggctccgc gagaaaaatc ggtcagcgcc agttgctggg ccaactcttt catcagggac
     4501 agcgcgcttt gcgaagggtc gggctgctgg ttggctacgt tcacgggcca ccacgggctg
     4561 gtgacgctga cttcgccacc accgcgcttg agcacgcgca ggcaaaaggc atcgcggttg
     4621 aggtcgtcgc cacgggcatt tttgccgcgc acggtgtttt tggcacggct ttcagccgcg
     4681 cgcaactcgc gcagcaccat acccagtggc gcgctgtggt gcgccactac cgcccccatc
     4741 gaggcggtgg ccttgtgacc catcagcgtc atcagtcggc tggccgaaaa acggccattg
     4801 cctagatgct ggccgggtag caatccaaag ccacttttta gcagcaactt gcgctgtgcg
     4861 tcactgccgc ctttttccag ttggccaagg tgtttgccca agcccatact gtcatcgggc
     4921 gcaatgccgc tgtaggccag gcgcagcaac tgcatgcagt caaacaaatc atccactgcc
     4981 acaagcgcca gaacatcgtc gccaccggca taaagcaatt tgcctttgca gcattcctcc
     5041 acaacatggc gagccaggcg ggtcgaaaaa tcgttgagcg cttgcgaaat ggctccgtgt
     5101 cggccaggtg atactgggcg cttggtgtca aggtagactt tgagtgcctt gtcctggccc
     5161 tcaaatttgg ccatttcacc cagcaccttg ctgtgccagg tttgccggta actgagtttg
     5221 tagtcgtctt cgttgccagc cagccagcca cccatgcggt cgccgtccat caggatcagt
     5281 gcatagtagg cctcgggacg gttgtcaccc agcaagcggg cgatggtgcg gctgcggtgg
     5341 ttgctctctg cactgccatc gtcggcatct tccagcatgg ttggcaagcg gcgcagtgtg
     5401 tcaagctctg tttgtgatag ctgctcacgg ttgtacagcg tgcgtaccag ggcttttgga
     5461 agggtcaccg catcaaagtc tttggcgctg gcggccagca cctgcagggc cgaggtttgg
     5521 tcggtcgtca ggggttgatt gggatctgtc agcttttcca gcgtggtgga cagcgccaac
     5581 gcatgggtgc tgacaacaaa tcggcgcaca tcaccgccgg tgagtcggct gacgcgctcg
     5641 gcaaacagcg tgggccacag gcgcttgagg gtggcgatgg cgctcaagtg ttcgccgggt
     5701 ttgacccaag atttcttttt tttggccagt ttgccccaga cgctgtcggc catacggtct
     5761 ttgcggttca accccagcag agccccgtca tgggtcagcc actcgccatc gccggtcagg
     5821 gtgcagcggt ggccttcttc caaaacgggc gcaaacggcc gggtggcttt ggcagcaccc
     5881 agcgcccgct cggccaattc ataaacggcc gggtagagga tgccggtgtt gggctgccag
     5941 aactgcaggc cgtcgagctt gagttccttg gtcagcacct gccaggtggc ctcctgaaaa
     6001 acgccttgct ttttcaaatc ggggtgaatg ctgtctagag cggcatgcag gcgggcgctg
     6061 gcggcagcca aatccctgca accatcgccg tcaattggcc aggtggcagc ggcccaatag
     6121 gcttcgggga atccggcgag ttgtgtcttg atctgggcac gggtgacatc ggtcagcgcc
     6181 acacccgctg catcaaaggc gcgcttggcg gcctctgtgg tccattgctt tgctgcattt
     6241 ctgatagctt ccagcgcacg ttctgcaagg gctgcagcct gttttgatgg aacaatcgcc
     6301 atgaatttgt tgggcaggct ggcggcaaac agcggattgg tgtcggtgcg gtctgccagc
     6361 aactcggagt cgatgtcgtc aaacagtttc acaaatgcat cgccacccac ctcggggctc
     6421 atcagccagg catcgaccag cggcacgccg cgcagcgcgg ggaacaccat gcagtccggc
     6481 cccaaatggc tgatgatggg tttcatggcc tcaaacacca ggcaagacag caggtgcgac
     6541 cccgcccaca aatcggaggt tgagcgtgct tggccgataa atccctgcac cgggccaaaa
     6601 ctcatgacca gcagcgcagg ttgatcctgg ccggtgtcgt cgcccgccag cgcggtgtga
     6661 atggcggata cggtgtcgat atgcgaccag atgctgtggt ctggcacgcg tgagtcagcg
     6721 ggcagcatgg gccacagcac acccagctca ctggcgtgct gaccggcttc ggggccaaag
     6781 cgccaaaagg ccaggaaggt tttacgcaga ctgtcgtcgt cgtgctgaat caggctctca
     6841 aagtgtgctt ggctgacggc cttgatctgc tcaacgttga tgcccaagtc gagctttttg
     6901 agctcgatct tttcacccga cagcgggtga attaactcgg cctccttggt gaacttgacc
     6961 gcttcaaact gcgggtagcg gcgagcatcg tcgttgcgcg gccaactcgg gcggtcggca
     7021 gcagcggcca gccagtcggc acgcttgtcg aattccttgg cggaaatgtc cagcgcctgg
     7081 cgcagtcggc gtgccgtgcc gccctcgtgg ccctcggcat cacgcatcag gaccagttgc
     7141 ttttcagccg ggtcgtgtaa ccaagcggcg agtttcttgg tgtagttggt gttatgtgtc
     7201 atgaacttag cccttgcgga tgcgggtgat ctcagaagcc ggccgggcgt tcagaaacgc
     7261 atggacttgg ttccagactt caatctggtc attgattggc agttgggtca cctggctgcg
     7321 gtcaaaaaat gcctttggca tggcgctggg taaatgggtg atcaggccga aatactgacc
     7381 atccctgttt ttggcgacct tgaaacgcat ctggctggca agtcgcgcat tgggcaaacc
     7441 cgccaggccg tggttggtaa ccgggtaggc cagcacatgc cgagcttcaa cccggttatg
     7501 cggtgagccg ctggacaatt tgaatcgcgt tcggaaacca attttgagag ccgccaagcg
     7561 ctccagcacg cttttccatt ccttgaagcc ggtaaaaatc agcttgccct ctgcattgag
     7621 ctttttgtcg gcaaacacgc gccacactgc cgggcgaccg tcagtacaca agcccagtgc
     7681 gtgcggccac tcgcccgcaa gtcgatctgt cagggctgcg ctcaggctgc gtggtgcgga
     7741 ttgcagggcc aggccggagt cacacaagcc ttcaaacccc aacaaaccct caccttcgat
     7801 gtgcaacgaa ccccagccat tgcgggcacg tgagccgacc gagccaaacc acgctgccaa
     7861 ctgcatggct ttgcgcaagg tgccagcttc tgcgtcgggg cagcgcagtt tgaatgtcat
     7921 cggtgggcct tcgggcgcaa tggcgttgcg gttgccgtgg gtggtgacgg ggccaaatcc
     7981 cagataaaca tcggttccaa ccgacatcag ttgcccggtt tgcctgtttt tgacctctga
     8041 atgttgctgg gttgccatgt gcggcaacga tgtcagcgtg ccagtatccc aagtcgacaa
     8101 ccgcaactgc actttggacc gccccccgcc cgcatcatcc ccagcgctgc caaacaagct
     8161 gttctcaagc ctgagcattt cgcgatggtc gtaaccgaga tcggctgcct tcaccacccg
     8221 ccaccactgc cgcagcagcg ccttgaacgg tggggtgcgc cactgggctt gttgttctgc
     8281 attgcctaaa aaagccgggg tattgaaact gagctggtag ctcaattcgc gcatggtcat
     8341 ttgttttcct ccttgagcac cacatctgcg gcaaatcgta ctgcatcagg gtgcagggtt
     8401 aacccatagc gccggggccg cacgtcaccg tcgtggatgc ggtaggcctg ggcagcgggt
     8461 cccagcgcgg ttttcaggcg cttgtccagt ttggacttgc gtgctgaaaa atattcacca
     8521 tccatgccgt tgcgcaaagc ccgctcggtg ccttcaatat ccgccagatt gccggtaatg
     8581 cggcggtatt cgcgcaaata gcgtgtggcc cacagcgggt ctggcacgcc cttggtcggg
     8641 gcgctcaagg gctggttgcc cgccagggtt tcgcgtgcaa acaccgccag cagcgccagt
     8701 tcggccggtg gcagggcaat ttcgcgccgg cccgccagaa tgcactggcg ggccaaatcc
     8761 agcaccagtt gcgccggtgc cagcgctgcg cgggccgcag ccaccgtgtc gttgaagctg
     8821 gcaacacctg ccagcagact ggtgggcagg ccgtgccgca ggctgacaaa cgggatttca
     8881 gccagcgtga cctgcgccat cgcggtatcg gccagttggc catcgcgcgt ttgcagcacc
     8941 cggctgtagg gtgtcgggta aaaaaaatcg tagctcgact caaacggctc gctcaccagc
     9001 acatggctga gtcggtcctg tgggcggcca aacaggctca gcgcgtagcc caggtaaaag
     9061 cccatggttt tgcgcccacc ggcaatcgat gcgtgtagcg cgcagcccga atcggccgtg
     9121 atggcgcgca catgggcggt gatgaagtca gccgctgcac ggttgtcggc cggtgtgcgg
     9181 atgtcgttca tcggctggcc gctggcatcg cgcatgacgt ggatgtgact gcggtcgaac
     9241 ttgacaccgg gcaggtggta gtcggcgcag agtctgtgaa accagcccag gtcgtcactg
     9301 agcaaggaca gctcggcgcg caccttcggc gctggtgatc aggtgaactt cggttgggac
     9361 aaacggcgcg aactcgtcag cggccagtgc gtacagcgtc tcggtaacga tttggggggt
     9421 gaggccgctg actgccagca gcaggcggcg gggaaagtcg gcagattggg tgagcgcgac
     9481 ggggtgcggg acatcaatgg gtttcatgcg ccgcagtgta ggcgggcagg gtgtcggcgt
     9541 gccctgcggc aagcttgccg tggctggcca gacacatcac cggtgcctgc aacgctggct
     9601 tcctggtgat cctgatgaca tcaccatttc tggcggcaag tgtcggattt ctgcgctacg
     9661 ggtgcgatca ggcttttgcc tgatctgcgt cagccactgg cgctgtcggt taacgccagt
     9721 agcgacgcgg gggcagatcg tcacggctgt tgtagttgtg tttgaggtca gtgatgtgcg
     9781 cctccagccg gtcaagctct cgatcaagtc gctcgcgctc atagggatcg atacgaccat
     9841 tgcgtcgtgc gcgaagctcg tcgtcacgca cttgccttaa ctccatgatc agccggttgg
     9901 cctctggccg ggtcagggct ccggagcgaa tgccttggtc aatgcgagcc tcggcgattc
     9961 gtacccgttc gcgaatgctc tgggccattg cagcgccaga aaatcccagg cataaggcca
    10021 gcacgccgcc gaaccagcca cgccgattga attgcttgtt catgatttct cctgttgttc
    10081 agttttctgt ccattgcgct ccaacgccgg cttacatgac ttctgacgga ttgaatctgg
    10141 tcatgaagcg atttcctcgc agattattgc aaagtc
//
LOCUS       DAOSLL010000088        10099 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6477_length_10099_cov_4.448825, whole genome shotgun sequence.
ACCESSION   DAOSLL010000088 DAOSLL010000000
VERSION     DAOSLL010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10099
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6477_length_10099_cov_4.448825"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1601
                     /locus_tag="PLB25_05590"
     CDS             <1..1601
                     /locus_tag="PLB25_05590"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOE41086.1"
                     /translation="VLASLPELQAAAHGQALLSVDASQTVVRRIALVMAVNQQVVPSL
                     PIEMLRLATGEPAVHLAVDAHGVTSVTVADLRIPTQANGEVWLHFARRQEGQLARQVS
                     AAAVLQGRVAPDALSSKLVLVGLTGSGLQDKRNTPLGQLVPGMEIQAQLVESALDGRF
                     LLRPWWIKGVETVALLIMGALLVWLMPRTRRAGVAAWRLKLPGISGLLLLAGLVAWLV
                     TGWVLFAQLGWLFDAASGVLGYLAVLLCLLLSSMLKTDLDNRRLAANEQQLREDAARV
                     AGELAAARRIQLGSLPDAARLFAGESRFEFATLLEPAREVGGDLYDFFMIDAQRLCFV
                     VADVSGKGVPASLFMAVTKTLTKSFSLRLTGSVADVVMAANQDLSRENVGMMFVTLLL
                     GVLDVQTGELELVNAGHDSPWRISQGGVVEKIATPADTGGPPLCVVDDFVYLSQRVQL
                     SAGDTLCVITDGVTEAMNQTGEMFGGQRLQAALSATPMGQSLNQTMTRLRAEITGFVG
                     AAEASDDLTLLLLRWQPESPAAIA"
     gene            complement(1606..2229)
                     /locus_tag="PLB25_05595"
     CDS             complement(1606..2229)
                     /locus_tag="PLB25_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOE41087.1"
                     /translation="MLASGANELDLVIVPLLALLVYVGVQSTAVTERVVLLPDDAGKA
                     GVVVVSTANGEQVLSTAYASAQFSGRGQLVAGRDEPDQVSARYASTLAALPPRPESFS
                     VYFASNSITELTPESGQVLNRMKAALASYPAPEITVIGHTDRVGRLEDNDALSVKRAA
                     TVRDFLLGLVASGTLIDIAGRGEREPLVPTADEVPEPLNRRVEINLR"
     gene            complement(2207..2662)
                     /locus_tag="PLB25_05600"
     CDS             complement(2207..2662)
                     /locus_tag="PLB25_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012433088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOE41088.1"
                     /translation="MKQLILSALCLGTMLSLAPPLALAQDAGVVKLNKGSNLLERDGT
                     RLPITVGSKVRVNDRVLTGADGSVGIMLQDNTMLSAGPNSTLQMNKFAFDTTTHAGIL
                     DASVKRGTVAVISGKIAKATPQNVSFSTPSMTLGVRGTAFIIDAGQRSE"
     gene            2712..3143
                     /locus_tag="PLB25_05605"
     CDS             2712..3143
                     /locus_tag="PLB25_05605"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE41089.1"
                     /translation="MIYRFPADMTALPAVLAQLQALCAQSDPALVLRAAMAIEELFTN
                     SVFHGLRTEGAPAQVGLSVQQHNGSLQVCYEDGFLPFDPFLGLDKTLATLERSGEDRP
                     VGGLGRLIVRGLADQASYTRDNAINRIDMLFSPRARIAPNL"
     gene            complement(3353..4495)
                     /gene="mltB"
                     /locus_tag="PLB25_05610"
     CDS             complement(3353..4495)
                     /gene="mltB"
                     /locus_tag="PLB25_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HOE41090.1"
                     /translation="MSKPLLPRCLIAIFIIATCAIPARAQAGNHPKYKAKAKPAASPL
                     SLPGPSYALRNDAMQLAEDIALRRHLDAAWVRQTLAQARYIPSIAKAITPAPVGTAKN
                     WRVYRSRFIEPIRIKAGAAFWQAHAETLRRAEQETGVPPEIIVGIIGVETIYGQQMGS
                     YRVIDALATLAFDFPASHPRAAQRSAYFKDELENYLSLTQRNGIDPLSLRGSYAGAMG
                     MPQFMPSSWAKYAIDFDGDGRVDLFASPADAIGSVASYFKAFGWQPGLPTHYPVSFDQ
                     HKLDLDALMAPDILPTFSTTSFQAKGAVLGAAALQHKGNLALIELQNGADANSYVAGT
                     DNFYVITRYNWSSYYAMAVIELGQAVMQALGATPGVLPYLPKPTDF"
     gene            complement(4500..6527)
                     /locus_tag="PLB25_05615"
     CDS             complement(4500..6527)
                     /locus_tag="PLB25_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HOE41091.1"
                     /translation="MNRTSFSHLPRDTRDTLFMLLVIAWVVMPLLGQLPGWCSALAAL
                     VMLWRGVLAWQGQPLPSRWWRFGLLALATGATLVTFKTLLGRDAGVTMVVVLLALKTL
                     ELRARRDAFVVFFLSFFLMLTNFFYSQSLLTAAAMLLALLGLLTALVNAHMPVGRPPL
                     WRVARIAGGMTLLGAPIMVLLFLLFPRLSPLWGMPSDAMAGRSGLSAQMTVGNIATLA
                     LDDSVAMRISFDGPAPTQAELYFRGPVLSNFDGQRWQPLELLAPTGAAEAALRVEGPG
                     ISYQVTLEPNQQPWLLTLDATAQAPELPQGQARMTADLQWLLNRPVTELLRYKVTSHT
                     RFQHGSLQPTARLQAWLTLPPGYNPRTLQLARQMQRDAPAGSQGQYWVDQALELLRSG
                     GYVYTLEPGVSGRHSADEFWFDRKAGFCEHIASSFVILMRDLGVPARVVTGYQGGERN
                     GLDGFWTVRQSDAHAWAEVWLAGQGWVRVDPTGSVAPSRVGSFSRLQGPRGAIASALL
                     GNVSPEIALTLRAAWDMVNNRWNQWVLNYTQNTQFKLLQNLGFAAPSWEDLVYLLCGI
                     VVTASLAGAVLGLWERKRQDPWLRLLGQAAKHLQRAGIKAPANPTPRQLADVLDRHGN
                     TASAQALGNWLLRLEALRYSASANSRGAGFRRQLATLQRELKHLRPNAPNL"
     gene            complement(6541..7524)
                     /locus_tag="PLB25_05620"
     CDS             complement(6541..7524)
                     /locus_tag="PLB25_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HOE41092.1"
                     /translation="MMVKTLSQPYLHPLRYLKARVARWWQDRLPLADSITLTQRNVYI
                     LPTRPGLMLGVTLLVLLVASINYQLNLGYLLTFLLAGCALVAMHVSHATLRGVTMHLI
                     APEAIFAGAKAQFVVHLQNTRRWPRQALALAVQELGQWVWTDIAAQSAASVQLAWQPP
                     GRGLHRLPTLSAQTLFPLGTFRVWTLWRPAAQVLVYPKPEPGAPPLPSGQSQDGKSQT
                     GVAHRQGDPDGLRAYRRGDALKSIYWKKAARTGELVSRDNLALQQRELWLDRQQTALS
                     HPEQQLSRLCAWVLQADQAGLRYGLRLAQQDIAPDSGPAHQQRCLRALALA"
     gene            complement(7597..8517)
                     /locus_tag="PLB25_05625"
     CDS             complement(7597..8517)
                     /locus_tag="PLB25_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013540684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE41093.1"
                     /translation="MDIQEKLTSLLNQLNTVIVGKPEQTQDCVACLLAGGHLLIEDVP
                     GVGKTTLAHALARTFGLQFSRVQFTADLMPGDLLGVSVYERNQEKFIFHPGPLFAQVL
                     LADEINRASPKTQSALLEAMEEKQVTIEGQTRALPLPFFVIATQNPMDQAGTYALPES
                     QLDRFLMCISLGYPSRDAERQLLCGVDRRDLVNALPSLLTAQELLVLQNKVQAVHAAG
                     PLLDYVQDLMGATRSGKWFAQGLSPRAGMAVLRAAKARALLAGRNYVAPDDVRAILPQ
                     TIAHRLIPTGEAGRGAAEQVRAMLDAVPLP"
     gene            8561..9514
                     /locus_tag="PLB25_05630"
     CDS             8561..9514
                     /locus_tag="PLB25_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOE41094.1"
                     /translation="MTMTGYFTHPSFRKHEMGPGHPECPERLDAIDDRMLISGLDVAL
                     DRREATQAATSDIELAHDRMYVAAIRGLNAELSEEIDAGGPTHLQIDPDTSVNIHTWA
                     AALNAAGAAIDATDAVMAGEMANAFCAVRPPGHHACRGKAGGFCFFNNVAIAAKYALE
                     RHNLQRVAIVDFDVHHGNGTEDIVKDDNRILMVSFYQHPFYPMGWNHSSAPNLVNLPV
                     PAYTKGKEIREMIEAAWMPRLEEFRPEMIFISAGFDAHREDDMGQLGLVESDYAWITS
                     RIKDVALRHSKGRIVSCLEGGYVMGSLSRSVEAHIRVLADV"
     gene            9507..>10099
                     /locus_tag="PLB25_05635"
     CDS             9507..>10099
                     /locus_tag="PLB25_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein"
                     /protein_id="HOE41095.1"
                     /translation="MSKPGPVDLEGWFEALTHPSSLIALGALLACGLMAWLLTSLFAR
                     AFEGRDAKSIMFGRRNVDGVLFPALLLALAYLARTLLANSVPAAVFKVAIPVLLSLLV
                     IRLAVKVMQAAFNETPLIRLFERTISWLAWLAMVLWVSGLLPLVLDELDQISWKVGGS
                     NLTVRNIIEGSLTAGLVLIITLWISSAIESQLLRQATG"
BASE COUNT         1823 a         3017 c         3257 g         2002 t
ORIGIN      
        1 gggtgctggc aagcttgccc gaactgcaag cggccgcgca cgggcaagcc ttgctgagcg
       61 tggatgccag ccagacggtg gttcgccgga ttgcgctggt gatggcggtc aatcagcagg
      121 tggtgccatc gctgccgatt gaaatgctgc gcctcgccac tggcgaaccg gccgtgcacc
      181 tggctgttga cgcgcatggt gtgaccagcg tgacggtagc tgacttgcgc attccgaccc
      241 aggccaacgg tgaggtgtgg ctgcattttg cccgccgaca agaaggccaa ctggcgcgtc
      301 aggtgtcggc cgcagcggtg ctgcaggggc gggtagcgcc tgatgcgttg agcagcaagc
      361 tggtgctggt ggggttgact ggctcaggcc tgcaggacaa acgcaacacc ccgctggggc
      421 aactggtgcc gggcatggag atccaggcgc agttggtcga aagtgcgctg gacgggcgct
      481 ttttgctgcg gccctggtgg atcaaaggcg ttgagactgt ggcgctgctg atcatgggcg
      541 ctctgctggt ctggctgatg cccagaaccc ggcgcgctgg cgttgccgcc tggcggctga
      601 aactgcctgg catctcaggc ctgttgttgc tggctggact ggtagcctgg ctggtcaccg
      661 gctgggtgtt gtttgcccaa ttgggctggc tgtttgacgc tgcctctggt gtgctgggtt
      721 atctggcggt gctgctttgc ctgctgttga gttcgatgct gaaaaccgat cttgacaacc
      781 gtcgcctggc tgccaacgag cagcagttgc gcgaagatgc ggcgcgcgtt gccggtgagc
      841 ttgctgccgc ccggcgcatt cagttgggct ctttgcccga tgccgcacgc ctgttcgccg
      901 gcgagtcgcg ttttgagttt gccactctgc tggaaccagc ccgcgaggtc ggcggtgacc
      961 tgtatgactt tttcatgatc gacgcgcagc gcctgtgttt tgtggtggct gacgtgtcgg
     1021 gcaaaggggt gcccgccagc ttgttcatgg cggtcaccaa aaccctgacc aagagttttt
     1081 cgctgcggct caccggcagc gtggccgatg tggtgatggc tgccaatcag gacctgtcac
     1141 gcgaaaacgt cggcatgatg tttgtcaccc tgttgctcgg cgtgctcgat gtgcaaaccg
     1201 gcgaactcga actggttaac gccggacatg actcgccctg gcgcatcagt cagggtggcg
     1261 tggttgaaaa gatcgcaaca cccgctgaca ccggtggtcc gccgctgtgc gtggtggacg
     1321 attttgttta cctgtcgcag cgcgtgcaac tcagcgcagg cgacacgctg tgcgtgatca
     1381 ccgatggtgt caccgaggcc atgaaccaga ctggcgagat gtttggtggc caaaggctgc
     1441 aagcagcgct gtcggctacg ccaatggggc agagcctgaa ccagacgatg acgcggctgc
     1501 gggcagaaat caccggtttt gttggcgcag ccgaagcctc ggatgacctg accctgctgc
     1561 tgttgcgctg gcagccagag tcacctgcag cgattgcctg accccttaac gaaggttgat
     1621 ttcaacgcgt cggttcaagg gctcgggcac ttcgtcggcg gtgggcacca gcggttcgcg
     1681 ctcgccgcgt ccggcaatgt cgatgagcgt gccggatgcc accaggccga gcagaaaatc
     1741 gcgcaccgtg gcggcgcgct tgactgacag ggcatcgttg tcttcaaggc gaccgacccg
     1801 gtcggtgtga ccaatcacgg tgatttcagg agccgggtag ctggccaaag ctgccttcat
     1861 ccggttcaga acctgccccg actcgggtgt cagttcggtg attgaattgg atgcaaagta
     1921 caccgagaac gattcgggtc ggggcggcaa ggctgccagt gtcgaagcgt aacgcgcgct
     1981 gacctgatcg ggttcgtcac ggcccgccac aagctggccc cggcccgaaa actgggcgct
     2041 ggcataggca gtgctcagca cttgctcgcc attggctgtt gacaccacca ccaccccggc
     2101 cttgccagca tcgtcgggca acaaaaccac ccgttcggta accgccgtgc tttggacacc
     2161 gacataaacc agtaatgcca gcagcggaac aatgaccagg tccaactcat tcgctccgct
     2221 ggccagcatc gatgatgaag gcggtgccgc gcacccccag cgtcatcgac ggggttgaaa
     2281 aactcacgtt ttgcggtgtg gctttagcga tcttgccgga aataaccgcc accgtgcccc
     2341 gcttcaccga ggcatcaagg atgcctgcat gggtcgtcgt atcaaaggca aacttgttca
     2401 tttgcaaagt ggaattgggt ccggctgaaa gcatggtgtt gtcttgcaac atgatgccaa
     2461 ccgagccatc agcgccggtc aaaacgcggt cattgacccg gactttgctg ccgacggtga
     2521 ttggcagccg ggttccgtcg cgttccagca ggttgctgcc tttgttcagt ttcaccacgc
     2581 cggcatcttg cgccagcgcc agcggcgggg ccagcgacaa catggtgcct aggcataatg
     2641 cagacaaaat gagctgtttc atttgtttcc ttttttcaag cggggcgtaa gccgtgaacc
     2701 aggcggcggc catgatatat cgttttcctg ctgacatgac ggccctgcca gccgtgctgg
     2761 cgcaactgca ggccctgtgc gcccaaagtg acccggcact ggtgctgcgg gctgccatgg
     2821 caattgagga attgttcacc aacagcgtgt ttcatggttt gcgaacagaa ggcgcgccag
     2881 cgcaggttgg cctgtcggtg cagcagcaca acggttcgct gcaagtgtgc tacgaggatg
     2941 gttttcttcc ttttgatccg tttctggggc tcgacaaaac cctggcaacg cttgagcggt
     3001 ctggggaaga ccggccggtc ggcggcctgg gtcggctgat tgtgcgcggg ctggcagacc
     3061 aggccagcta cacgcgcgac aacgcgatca accggattga catgctgttt tcgcccagag
     3121 ccagaattgc cccaaacctg tagttgccac cggtgcccgg cacattggag gattggcgcg
     3181 caagttctgg acagatcgcg tcgcaaccgc tttggctgat tcgattgcca aaccatccat
     3241 gtcgctgtga cctggccttg ccccaggttt gcaaggtctg ccgccggggt gcttgacccc
     3301 ttatgaagca tcggctgcga accgagcgcg gatcgcatgt gccgcgctga tctcagaagt
     3361 cggtcggctt tggcaggtac ggcagaactc cgggtgttgc ccccagcgct tgcatcacgg
     3421 cctgtcccag ctcgatcacc gccatcgcgt agtagctact ccagttgtag cgggtgatga
     3481 cgtagaagtt gtcggttccg gccacgtaac tgtttgcgtc ggcaccgttt tgcaattcaa
     3541 tcagtgccag attgcccttg tgctgcaggg cggcagcgcc cagcactgcg cctttggcct
     3601 gaaagctggt ggtgctgaag gtcggcaaaa tgtccggtgc catcagggca tcgaggtcga
     3661 gcttgtgctg gtcaaaactg accgggtaat gggtcggcag gcccggctgc cagccaaacg
     3721 ccttgaagta gctggccact gagccaatcg catcggccgg gctggcaaac aggtcaacgc
     3781 ggccatcgcc atcaaagtca atggcgtatt tggcccagct cgagggcatg aactgcggca
     3841 tgcccatggc accggcgtag ctgccgcgca ggctcaacgg gtcgatgccg ttgcgctgcg
     3901 tcaggctcag gtagttttcc agctcgtcct tgaagtaggc gctgcgctgt gctgctcgcg
     3961 ggtggctggc cggaaagtca aacgccagcg tggccagggc gtcaatcacc cggtaactgc
     4021 ccatttgctg accataaatg gtttccacac cgatgatgcc gacgatgatc tcgggcggca
     4081 cgccagtttc ctgctcggca cgcctgaggg tttcggcgtg ggcttgccag aatgcggcac
     4141 ctgccttgat ccggatgggt tcgatgaacc ggctgcggta aacccgccag tttttcgcgg
     4201 tgccaaccgg cgccggcgtg atggctttgg cgatgctggg gatgtagcgg gcttgcgcca
     4261 gcgtctggcg tacccaggcg gcatcgaggt ggcggcgcag ggcgatatct tcggccagtt
     4321 gcatggcatc gttgcgcagc gcgtagctcg ggccgggcag gctcagcggg ctggcagcgg
     4381 gcttggcttt tgccttgtat tttggatggt ttccggcttg cgcccttgct ggtattgcgc
     4441 aagtagctat aataaaaata gcaattaggc agcgcggaag aagtggcttg ctcatcaggt
     4501 cagaggtttg gggcgtttgg gcgcaggtgt ttgagttcgc gctgcagtgt agcgagctgg
     4561 cggcgaaacc cggcaccgcg gctgtttgcc gaggcactgt agcgcagtgc ctccagccgc
     4621 agcagccagt taccaagcgc ctgcgcagat gccgtgttgc cgtggcgatc cagcacatca
     4681 gccaattggc ggggtgtcgg gttggccggg gctttgatgc cagcgcgctg caggtgtttg
     4741 gccgcttgcc caagcagtcg cagccacggg tcctggcgct tgcgttccca caggcccagc
     4801 accgcgcctg ccagactggc ggtcaccacg atgccacaca gcaggtacac caggtcttcc
     4861 cagcttgggg cggcaaagcc gaggttttgc agcagcttga actgggtgtt ttgggtgtag
     4921 ttcagcaccc actggttcca gcggttgttg accatgtccc aggcagcgcg cagggtcagg
     4981 gcaatttccg ggctgacatt gcccagcagg gcgctggcaa tggcaccgcg cgggccttgc
     5041 agccgggaga aactgccaac ccgcgacggt gccaccgagc cggtcgggtc cacccgcacc
     5101 cagccttgtc cggccagcca gacctcggcc caggcgtggg catcgctctg gcgcaccgtc
     5161 cagaagccat cgagcccatt gcgttcgcca ccctgataac cagtgaccac ccgcgccggt
     5221 acgcccaggt cacgcatcag gatgacaaag ctgctggcaa tgtgttcgca aaagccggcc
     5281 ttgcggtcaa accagaattc gtcagcactg tgccgtccag acacgcccgg ctccagcgtg
     5341 taaacgtagc cgccactgcg caggagttcg agcgcctggt caacccagta ctggccctgg
     5401 ctgcccgcag gcgcgtcgcg ctgcatttgg cgcgccagtt gcagggtgcg cgggttgtag
     5461 ccgggcggca gcgtcagcca ggcttgcagg cgtgcagtgg gctgcagcga accgtgctga
     5521 aaccgggtgt ggctggtgac cttgtagcgc agcagttcgg tcaccggccg gttcagcagc
     5581 cactgcaaat cggccgtcat gcgggcctgg ccctggggca gttcgggcgc ttgcgcggtg
     5641 gcatcgaggg ttagcagcca gggctgctgg ttgggctcaa gcgtcacctg gtaactgatg
     5701 cccggacctt ccacccgcaa ggcggcctca gccgcgccag tgggtgccag cagttccagt
     5761 ggttgccagc gttgcccgtc aaagttcgac agcaccggcc cgcgaaagta cagttcggcc
     5821 tgggttggcg cggggccgtc aaagctgatg cgcatcgcca cgctgtcgtc aagcgccagt
     5881 gtggcgatgt tgccaaccgt catttgcgcc gacaagccac tgcgcccggc catggcatcg
     5941 ctgggcatgc cccacagtgg cgagagtcgc ggaaacagca aaaacagcag caccatgatt
     6001 ggcgcgccca gcagcgtcat gccaccggca atgcgcgcca cccgccaaag tggtggtcgg
     6061 cccaccggca tgtgggcatt gaccagcgcg gtcagcagcc ccagcagcgc cagcagcatg
     6121 gcagcggcag tcagcagcga ctgcgagtaa aaaaaattgg tcagcatcaa aaagaagctg
     6181 agaaaaaaca ccacaaaggc atcgcggcgg gcgcgcaact ccagtgtctt gagcgccagc
     6241 aataccacca ccatggtgac cccggcatcg cgcccaagca gggtcttgaa ggtgacgagc
     6301 gtggcaccgg tggccagcgc cagcagcccg aaacgccacc atcgcgacgg cagcggctgc
     6361 ccctgccagg ccagcacgcc acgccacagc atcaccaggg cggcgagtgc gctgcaccac
     6421 cccggtaact ggcccagcag cggcatgacc acccaggcaa tcaccagcag catgaacagc
     6481 gtgtcccggg tgtcgcgcgg caggtgggaa aagctggtgc ggttcatgac tgcctggtgt
     6541 tcatgccagc gccagtgccc gcaggcagcg ctgttggtgg gccgggccgc tgtcgggcgc
     6601 gatgtcttgc tgcgccagtc gtagcccgta gcgcaagccg gcctggtcgg cctgcagcac
     6661 ccaggcacac aggcggctga gctgttgctc ggggtggctt aaggcggtct gctgacgatc
     6721 aagccagagt tcgcgctgct gcagtgccag gttgtcacgg ctgaccagct caccggttct
     6781 ggcggccttc ttccagtaaa tggatttcag cgcatcgccc cggcggtagg cgcgcaggcc
     6841 atcggggtcg ccctggcgat gggccacacc ggtctgactc ttgccgtctt gcgactggcc
     6901 cgacggcaac ggtggcgcgc ccggctccgg cttcgggtac accagcacct gcgccgctgg
     6961 ccgccacagc gtccagaccc gaaaggtgcc gagcgggaac agggtttgcg cgctcagcgt
     7021 gggcagccga tgcagaccgc gtccgggtgg ctgccaggcc agttgcacgc tggcagccga
     7081 ctgcgcagcg atatcggtcc agacccactg cccaagctcc tgcaccgcca gtgccagggc
     7141 ctgacgcggc caccgccggg tgttttgcaa atgaacaaca aattgggctt tggcccccgc
     7201 gaatattgcc tcaggagcta tcaaatgcat agtgacaccg cgcagtgtgg cgtggcttac
     7261 atgcattgcc accagcgcgc aaccggccag cagaaaagtc agcaagtagc ccagattgag
     7321 ctggtaattg atcgaggcaa ccagcagcac cagcagggtt acacccagca tcagtccggg
     7381 tcgggtcggc aaaatataga cattgcgctg tgtcagcgtg atgctgtcgg ccagcggcaa
     7441 gcggtcttgc caccagcgcg ccacgcgggc cttgaggtag cgcaacgggt gcagataggg
     7501 ctggctcaag gttttgacca tcatgcgcct ggttttcctg ctcgactggg tgccgctgct
     7561 gacggttgtg tcaccgcagc catcgggtca ggccggtcac ggcagcggca ccgcgtcgag
     7621 catcgcgcgt acctgttcgg ctgcgccgcg accggcttcg ccggtgggaa tcaatcggtg
     7681 ggcgatggtc tgtggcagga tggcgcgcac gtcgtccggg gccacatagt tgcgcccggc
     7741 cagcagggcg cgtgctttgg cggcgcgcag caccgccatg cctgcccgtg ggctcagtcc
     7801 ctgggcaaac cacttacctg atcgggtggc tcccatcagg tcttgcacat agtccagcaa
     7861 cgggccagca gcatgcacag cttgcacctt gttttgtagc accaggagtt cttgcgcggt
     7921 cagcaagctg ggcagcgcat tgaccaggtc gcgccggtcc accccgcata gcagttggcg
     7981 ctcggcatcg cggcttggat agccgagcga aatgcacatc aaaaaccggt caagttgcga
     8041 ctctggcaag gcgtaggtgc cagcctggtc catcgggttt tgcgtggcaa tgacaaaaaa
     8101 tggcagcggc agcgcccgcg tctggccttc aatggtgacc tgtttttctt ccatggcctc
     8161 cagcagcgcg ctttgcgttt tcgggctggc gcggttgatc tcgtcggcca atagcacctg
     8221 cgcaaacaag ggccccgggt ggaagataaa cttctcctgg ttgcgttcat ataccgatac
     8281 ccccagcaag tcgccgggca tcaggtcggc agtaaattga acgcgcgaaa attgcagccc
     8341 aaacgtgcgg gccagcgcgt gcgccagtgt cgtcttgcca acaccgggca cgtcttctat
     8401 cagcagatga ccaccggcta gcaaacaggc cacgcaatct tgcgtttgct caggtttgcc
     8461 gacgatcacc gtgttaagct gattcaacag cgatgtcagt ttttcttgaa tgtccattgg
     8521 acgaccttac caaataatta gccataggag gcaatgacgt atgacgatga ccggatactt
     8581 tactcacccg agctttcgca agcatgaaat gggcccgggc catcctgagt gtcctgagcg
     8641 gcttgatgcc attgatgacc gcatgctgat ctccggcctg gatgtggcac ttgaccggcg
     8701 cgaggcaacc caggctgcca cttccgacat cgaattggcc cacgaccgta tgtatgttgc
     8761 agcgattcgc ggcctgaatg ccgaactcag tgaggaaatt gacgcgggtg gcccgaccca
     8821 tctgcaaatt gaccctgaca ccagcgtcaa catccacact tgggctgcgg cgctgaatgc
     8881 cgcaggtgcc gccattgatg ccaccgatgc ggtgatggct ggcgaaatgg caaacgcttt
     8941 ttgtgcggtg cgaccaccgg ggcaccatgc ctgtcgtggc aaagcgggtg gtttttgctt
     9001 cttcaacaat gtggcgattg ctgccaaata tgcgctggag cgacacaacc tgcagcgggt
     9061 ggcgattgtt gactttgatg tgcatcatgg caatggtacc gaagacattg tcaaggatga
     9121 caaccgcatc ttgatggtga gcttttacca gcacccgttt tacccgatgg gctggaacca
     9181 ttccagcgcg cccaatctgg tcaatttgcc ggtgccggcc tataccaagg gcaaggaaat
     9241 ccgtgaaatg atcgaagctg cctggatgcc gcggctggaa gaattcaggc ccgagatgat
     9301 ctttatcagc gccggttttg atgcccaccg cgaggacgac atgggccagc tcggcctggt
     9361 tgaaagcgac tacgcctgga tcaccagccg catcaaggat gtcgcgctgc gtcattcaaa
     9421 gggacgcatt gtgagttgtc tggaaggcgg atatgtgatg ggttccctgt cgcgcagtgt
     9481 ggaggcgcat atccgggttc tggccgatgt ctaagccagg gccggttgac ctggagggct
     9541 ggtttgaagc cctcacccat ccctcatccc tgatcgcgtt gggcgcactg ctggcctgtg
     9601 gcctgatggc ctggctattg accagcctgt ttgccagagc gttcgagggg cgtgatgcca
     9661 agtccatcat gtttggccgg cgcaatgtcg atggcgtgct gtttccggcg ttgttgctgg
     9721 ccctggccta cctggcccgc accctgctgg ccaattcggt tcctgcggca gtcttcaagg
     9781 tggccattcc agtgttgctg tccttgctgg tgatacgcct ggcggtcaag gtgatgcagg
     9841 cggcttttaa cgagacaccg ctgattcgcc tgttcgagcg caccatctcg tggctggcct
     9901 ggctggccat ggtgctgtgg gtcagtggct tgctgccatt ggtgcttgac gaactcgatc
     9961 aaatcagttg gaaggtcggt ggcagcaatc tgaccgtgcg caacatcatc gagggttcgc
    10021 tgacggccgg tttggtgctg atcatcacgc tgtggatttc gtcggccatc gagtcccagc
    10081 tgctgcgtca agccaccgg
//
LOCUS       DAOSLL010000089        10070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6502_length_10070_cov_5.280579, whole genome shotgun sequence.
ACCESSION   DAOSLL010000089 DAOSLL010000000
VERSION     DAOSLL010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10070
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6502_length_10070_cov_5.280579"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="PLB25_05640"
     CDS             <1..434
                     /locus_tag="PLB25_05640"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOE41096.1"
                     /translation="PSVPRPISAEGVIQGLRAIEYLDVDVGLARANNKPVFYASMLRK
                     FVASQEDALLRIHTALAQADAATAQRIAHTLKSVSGSLGATTLPKVAEELEMALRQGV
                     QGEDLKDAIDATLKALDNLMTGLKSTPGLLPPEMPVAATEK"
     gene            922..2235
                     /locus_tag="PLB25_05645"
     CDS             922..2235
                     /locus_tag="PLB25_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOE41097.1"
                     /translation="MKLKARLTLAAALALSLCGISNAATELVIATVNNGHMIEMQKHS
                     NAFEAANPDIKLKWVTLEEGVLRQRVTTDIATKGGQFDVMTIGMYETPIWGKKGWLTE
                     LKTNAAYDVDDLLPAMRNGLSVNGKLFAAPFYGESSMLMYRKDLADKAGIKVDDKPTW
                     TQVKDLAAKIHDPKNGVYGICLRGKPGWGDNMAFLSTLVNTNGGQWFDMNWKPQLESQ
                     PWKTAINFYVDLLKNYGPPGSSANSFNEILALYNEGKCGMWIDATIAASFITDPKQSK
                     VADKVAFAQAPTAVTPKGANWLWAWALAIPAGSQKVDAATKFVTWATSKEYIDLIGKE
                     VGWGNIPTGTRKSTYTRPEFLKVAKFAAAEKAAIDSANPNDATLPKSPYVGVQFAAIP
                     EFQAIGTTVGQQMSAALAGKSTVDAALKASQVAADREMKKAGYYK"
     gene            2549..3409
                     /locus_tag="PLB25_05650"
     CDS             2549..3409
                     /locus_tag="PLB25_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE41098.1"
                     /translation="MNRFIPRILLAPAVLTLFLWMIVPLVMSIYFSVIRYNLMQPDKT
                     GFIGLENFEYFMTDPSFGTAVVNTILLLGSVILISVIGGILLALLVDAPFPGRNMVRI
                     LLISPFFVMPTVNALLWKHMMMNPIYGVLAQVWIFFGATPVDWLTDWPLWSVILIVSW
                     QWLPFATLIFITALQSMDREQLEASTMDGATYLQQLRYLYIPHLGRSMAVVVMIEMIF
                     LLSVFAEIYTTTGGGPGDASTNVAFLIFKQALLNFDAGVASAGALFAVVLANIAGVFL
                     IRMVGKNLDK"
     gene            3417..4247
                     /locus_tag="PLB25_05655"
     CDS             3417..4247
                     /locus_tag="PLB25_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE41099.1"
                     /translation="MNTSLSKSALFYLRTLCAWGITLLLFFPLGWLVLTAFKTELQAI
                     SVPPLLFFPPTLENFTIVQERSDYLLYAKNSLITSVASTILGLALAFPAAYSMAFFKG
                     KYTKDILMWMLSTKMMPAVGALVPIYVLAQGSGLLDTRTGLVIIFTLSNLPIMVWMLY
                     SQFKEIPGEILEAARMDGASLWQEFRMVLLPLTLGGLASTGLLCLVLSWNEAFWSLNL
                     SAAKAGTLATLIASYSSPEGLFWAKLSAASFMAIAPIVVFGWFSQKQLVQGLTFGAVK
                     "
     gene            4277..5338
                     /locus_tag="PLB25_05660"
     CDS             4277..5338
                     /locus_tag="PLB25_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE41100.1"
                     /translation="MAYLKLERIEKIFGETRVIKGIDLDIRQGEFVVFVGPSGCGKST
                     LLRLIAGLEHINSGTLHLDGRDITNLPSSKRDLAMVFQSYALYPHMSVYENMSFALKL
                     AKADPAVISDKVARAAKILNLTEYLQRTPKELSGGQRQRVAIGRAIVRAPKVFLFDEP
                     LSNLDAALRGQTRIEIAKLHRDLGATTIYVTHDQVEAMTLADRVVVLRDGLIEQVGTP
                     LELYDRPANQFVAQFIGTPQMNIVAAAQLPALDAVGALGIPAGGFIGLRPENVILHPA
                     AQGLLKGTVDLVEALGAETLIYITTAQGAQLVSRQNERTPLRAGDAVGVQIDTEAAHV
                     FDAKGRIIRTGQTHETPTH"
     gene            5360..6094
                     /locus_tag="PLB25_05665"
     CDS             5360..6094
                     /locus_tag="PLB25_05665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE41101.1"
                     /translation="MNPTQPLSGQVAAITGSASGIGFASAQAMIAAGAQVVLIDRDAP
                     ALAKAREALGSAALPLVLDLLDPAQCAAMPARILALAGRLDIFHANAGLYVGGDLIDA
                     NDDAIDRMLNLNINVVMKNVRGVLPHMIERGSGDIIVTSSLAAHFPTPWEPVYASSKW
                     AIDCFVQTVRRQVFKHGIRVGAISPGPVITALLADWPAEKLAEAKARGSLLEASEVAN
                     VVLFMLTRPRGMTIRDVVMMPSQFDL"
     gene            6151..7536
                     /locus_tag="PLB25_05670"
     CDS             6151..7536
                     /locus_tag="PLB25_05670"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HOE41102.1"
                     /translation="MYTILHLGLGSFHRAHQAVYLHQLQQTGDRSWALAGGNIRPDMA
                     DTIAALIAQGGAYTLETVTPHNQRSYTRITAIQTVVPFDAELSGLVRIGSDANTRIIS
                     FTVTEAGYYLDAKYQLDQNFADLRADLAAVKAGEPVCTIYGALTVILRARMAANAGPV
                     TLLNCDNLRHNGERSRGGLLQFIELLGDSALLTWVNANTTSPNAMVDRITPRPTPAVR
                     ERVLAATGVDDAAALMGESFIQWVIEDNFIAGRPAWESVGVEMVDSVQAYEEAKIRLL
                     NATHSCIAWAGTLVGYTYIHEGTLDPVIRQFAYDYVTDDTIPVLLPCPINLETYRDVV
                     LDRFGNPAIADTNQRVAMDAFSKIPAMIAPTIRDRLARGESINSVAMLPALFLAYLQR
                     WHAGQIPYTYQDQAMDPAVAHAICEATDPVAAFCADLTLWGPLACDERLIDALRQAFT
                     RVTQFVQNHQK"
     gene            7594..8517
                     /locus_tag="PLB25_05675"
     CDS             7594..8517
                     /locus_tag="PLB25_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOE41103.1"
                     /translation="MTSLPRQLKPELEHDYVRSPDLGYESPDEPGFIRCLSHGFPTPL
                     ARWHQHDEYELHLITATSGKVFVGDWIGHFQPGHLVLTGPRLPHNWVSTDVPEGGVAE
                     RDLVIQFLHAPIETGSQHIPELREVLPLLERAKHGIEFFGIFPQALAHWQRVKVHQGL
                     ARLAAFCDFLSELTQCTDYRLLSSTQMQSVDNDAQLEQINAIVSRINEHLSDPLSAAD
                     LARELDMSESAFSRFFRRATGNTFTDFVNHVRVSRACQLLMESDLLINHIGYQVGFNN
                     IANFNRRFLDIKGMTPSDYRRQAASRFGLKA"
     gene            8542..10002
                     /gene="xylB"
                     /locus_tag="PLB25_05680"
     CDS             8542..10002
                     /gene="xylB"
                     /locus_tag="PLB25_05680"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HOE41104.1"
                     /translation="MYLGIDLGTSGLKLLLLDQQHHIHASVDAAITLQRPHPTWSEQH
                     PHDWWQALESAVAQLRAAAPQAWRQVRAIGLSGQMHGAVVLDENAKVLRPAILWNDGR
                     SPAQCLELERLVPNSRQITGNLAMPGFTAPKLLWLREHEPTVFEQIRTVLLPKDWLRL
                     QLTGEAVSEMSDASGTLWLDVGKRCWSETMLAACGLDLSHMPRLVEGSQPSGCLQGAL
                     AQRWGLTASVVVAGGGGDNAASAVGIGAANPGQGFVSLGTSGVVFLVSNVYQPAPELA
                     VHAFAHALPGRWHQMSVMLSAASAFGWVTQLTGSPDEAHLAEQVAALPLSQRAGAPIF
                     LPYLSGERTPHNNPNASGVFIGLRGEHAAADLGYAVMEGVSFGLMDGLRAMGQTEAST
                     VPLALVGGGARSDAWAQLLASGLACPLHRPEGAHAAAALGAARLAWLADGGTEQEVCQ
                     PLPTEQAFAPEPGCRELLAERYPRYRALYPALRDFF"
BASE COUNT         1972 a         3071 c         2950 g         2077 t
ORIGIN      
        1 agccttcggt gccaagaccc atcagtgccg aaggcgtgat ccagggcttg cgggccattg
       61 aatatctgga tgtagacgtg ggtctggcgc gggccaacaa caaaccggtt ttttatgcct
      121 cgatgctgcg aaaattcgtc gcctcgcagg aggatgcgtt gctgcgaatc cacacggctc
      181 tggcgcaggc agatgccgcc actgcacagc gcatcgcgca tacgctcaag agtgtgtcgg
      241 gcagtctggg tgccaccacc ttgccgaagg tggctgagga attggaaatg gccttgcgcc
      301 agggggtgca gggagaagac ctgaaagacg ccattgacgc gactttgaag gcactcgaca
      361 acctgatgac aggactcaag tcgactccag gcctgctgcc gcccgagatg cctgttgcgg
      421 caacagaaaa atgagccgac tcgtgggttg cagttgtggg ccgcttccct ttctcctggg
      481 catccaggca ttgacggcca accttgccat gaggccgcgt tgcaactttt gcacgctggt
      541 gccacagtca actgatatcg gaattgacca ggatctgccg attcgccgtt gacgcaatgg
      601 ccttttgcat cactgaagcg gcatcaacca gcgccgattt tggacttttg gcaccgctgt
      661 agcgatccag cattggttga ctgctgggtg aacccggaca accagtggca cagcatcggg
      721 acagcttgcc aacctggcaa tacaaatatg ccgccactag gtataaatcc taggtatgtt
      781 tggcaaataa gtaccatatt acctggtatg agtaccaaac atgcaataaa agatcagtat
      841 cgttgaggat gtcagtgcat gccttgcact gagcaaagcc aacaacatca cccactctct
      901 ctttctctca ggagacccag catgaaactg aaagcacgcc ttacccttgc agccgctttg
      961 gccctgtcgc tttgcggcat atccaacgca gccaccgaac tggtcattgc caccgtcaac
     1021 aacggccaca tgatcgaaat gcaaaagcac agcaacgcgt ttgaagccgc caacccggac
     1081 atcaagctca aatgggtcac gctcgaagaa ggtgtgctgc gccagcgcgt cacgactgac
     1141 attgccacca agggtgggca gtttgatgtg atgaccatcg gcatgtatga gaccccgatc
     1201 tggggcaaaa aaggctggct gaccgaactc aagaccaatg ccgcttatga cgtggacgac
     1261 ctgctgcccg ccatgcgcaa tggtctgagt gtgaatggca agctgtttgc cgcaccgttt
     1321 tatggtgaaa gctcgatgtt gatgtaccgc aaggacctgg ccgacaaggc tggcatcaag
     1381 gttgatgaca agcccacctg gacccaggtc aaggacctgg ctgccaagat acatgacccg
     1441 aaaaatggtg tctatggcat ctgcctgcgc ggcaaaccgg gctggggcga caacatggct
     1501 ttcctctcca ccctggtcaa caccaatggc ggtcagtggt ttgatatgaa ctggaagccg
     1561 caactggaaa gccagccctg gaaaaccgcc atcaactttt atgttgacct gctgaaaaac
     1621 tatggcccgc ccggttcgtc tgccaacagc ttcaacgaaa ttctggcgct ctacaacgaa
     1681 ggcaagtgcg gcatgtggat tgatgccacg attgccgcat ccttcatcac cgacccgaaa
     1741 cagtccaaag tggctgacaa ggtagccttt gcccaagccc ctaccgcagt aacgcccaag
     1801 ggtgccaact ggctgtgggc ctgggcgctg gcgattccgg ccggttcaca aaaggttgac
     1861 gctgccacca agtttgtcac ctgggccacc agcaaggaat acatcgacct gattggcaag
     1921 gaagtgggct ggggcaacat cccaaccggc acccgcaaga gcacctacac caggcctgag
     1981 ttcctgaaag tggccaagtt tgccgccgcc gaaaaagcgg ccatcgacag cgccaacccg
     2041 aacgatgcca ccttgcccaa gagcccgtat gtcggcgtgc agtttgctgc catcccggaa
     2101 tttcaggcga ttggcaccac agttggccag cagatgagcg ccgccctggc aggcaagtca
     2161 acggtggatg cagccctgaa ggcatcccag gtggcagccg accgcgaaat gaagaaggcc
     2221 ggctattaca agtaagttgg caataggaca gcggtaggaa ctgcggcagc cgcgatgctc
     2281 gcgccttgcc gtgctcctac aagtcaaaaa cagccatgta ggtcgggtta gcgaagcgta
     2341 acccggcggt tttatgcgct gtagcctgcg tgcccaagtc gggttagccg gtacggctag
     2401 cccgatctga ttctatcaaa ttagtagcta tcagcgcttg ttccataagg gctggaggtt
     2461 gatttgatca tcaattcgat agagggcgca caggcttggc ttgcgcattc tctggccttt
     2521 gttaactgat tcaccgaggc gtttgtccat gaaccgattc attccccgca ttctgctggc
     2581 accggcagtg ctcacgctct tcctgtggat gatcgtgccg ctggtgatgt cgatctattt
     2641 ctccgtaatc cgttacaacc tgatgcagcc cgacaagacc ggctttatcg ggctggagaa
     2701 ttttgaatat ttcatgaccg acccgtcctt tggcacggcg gtggtcaata ccatcttgct
     2761 gctcggcagc gtgatcctga tctcggtgat aggtggcatt ttgctggcct tgctggtcga
     2821 tgcacctttt ccgggtcgca acatggttcg cattttgctg atctcaccgt tcttcgtcat
     2881 gccaacggtc aacgcactgc tgtggaaaca catgatgatg aacccgattt acggggtgct
     2941 ggcgcaggtc tggattttct ttggtgccac cccggttgat tggttgaccg actggccgct
     3001 gtggagcgtg attttgatcg tttcctggca gtggctgccg tttgccacgc tgatctttat
     3061 taccgcactg caaagcatgg accgcgagca gctcgaagca tcaaccatgg acggggccac
     3121 ctatttgcag caattgcgct atctctacat cccgcacctt ggccgctcca tggcggtggt
     3181 ggtgatgatc gagatgatct ttttgctcag cgtgtttgcc gagatttaca ccaccaccgg
     3241 cggtggtccg ggcgatgcca gcaccaatgt ggcgttcttg atcttcaagc aggcgctgct
     3301 gaactttgat gccggtgtgg cctcggcggg tgcgctgttt gctgtggtgc tggctaatat
     3361 tgccggcgtg ttcctgatcc gcatggttgg caaaaacctt gacaagtgag cacaacatga
     3421 acacttccct ttccaaatca gcgctgtttt acctgcgcac gctctgcgcc tggggcatta
     3481 ccctgctgct gttttttccg ctgggctggc tggtgctcac cgccttcaag accgaactgc
     3541 aggcgatctc ggtgccgccg ctgctgtttt tcccgccaac gctggagaac ttcaccatcg
     3601 tgcaagaacg cagtgattac ctgctgtatg ccaaaaactc gctgatcacc agcgtggcgt
     3661 ccaccattct cgggctggcg ctggcttttc ctgccgccta ttcgatggcg tttttcaagg
     3721 gcaaatacac caaagacatt ttgatgtgga tgctttcgac caagatgatg ccggccgtcg
     3781 gtgcgctggt tcccatttat gtgctggcgc aaggctctgg actactcgat acccgcaccg
     3841 gtctggtcat catctttacc ttgagcaacc tgccgatcat ggtctggatg ctgtattcgc
     3901 agttcaagga aatccccggc gagattctgg aagcggcgcg catggacggg gccagcttgt
     3961 ggcaggaatt tcgcatggtg ctgctgccgt tgacgctcgg cggactggca tccaccggcc
     4021 tgctgtgcct ggtgctgagc tggaatgaag ccttttggtc actcaatttg agcgccgcca
     4081 aggccggcac gctggccacg ctgattgcca gttactccag ccccgagggc ctgttctggg
     4141 ccaagctgtc tgctgcatcg ttcatggcaa ttgcgccgat tgtggtgttt ggctggttca
     4201 gccagaagca actggtgcag ggcctgacct ttggtgccgt caaataaatc gttttaagaa
     4261 tactggagac ctcttcatgg cttacctcaa acttgagcgg attgagaaaa tatttggcga
     4321 aacccgcgtc atcaaaggca ttgaccttga catccgccaa ggcgaatttg tcgtgtttgt
     4381 cgggccgtca ggctgcggca aatcgacgct gctgcgcctg attgccgggc tcgagcacat
     4441 caacagcggc accctgcatc tggatggccg cgacatcacc aatttgccat cgagcaagcg
     4501 tgacctggcc atggtgttcc agagctacgc gctgtacccg cacatgagcg tgtacgagaa
     4561 catgagcttt gcgctgaaat tggccaaggc agacccggcg gtgattagcg acaaggtggc
     4621 gcgcgccgcc aaaattctga acctgaccga gtacctgcag cgcacgccca aggaactgtc
     4681 gggcgggcag cgccagcgcg ttgccattgg ccgtgccatc gtgcgtgcgc ccaaggtgtt
     4741 tctgtttgac gagccactgt caaacctgga cgcggcgctg cgcggccaga cccgcatcga
     4801 gatcgccaaa ctgcaccgcg acctcggtgc cacgacgatc tatgtcaccc acgatcaggt
     4861 cgaggcgatg accctggcgg accgggtggt ggtgctgcgc gacgggctga tcgagcaggt
     4921 tggcacgccg ctggagctgt atgaccgccc ggcgaaccag tttgtggccc agttcattgg
     4981 cacgccgcaa atgaacatcg tggctgcggc ccagttgccc gcactcgatg cggtgggcgc
     5041 gctaggcatt ccggcaggcg gctttatcgg cttgcggcca gaaaacgtga tcttgcaccc
     5101 ggctgcgcaa ggcttgctca agggcaccgt tgacctggtc gaggcgctcg gtgctgaaac
     5161 cctgatttac atcaccacgg cgcaaggcgc gcaactggtg tcacgccaaa acgagcgtac
     5221 cccgttgcgc gcgggtgatg ccgtcggcgt gcaaattgac accgaagcgg cccatgtgtt
     5281 tgatgccaaa gggcgcatca tccgcaccgg ccagacccat gaaacaccca cgcactgatc
     5341 aaccaacccc cgcaacacca tgaacccaac acaaccttta agcggccagg tggccgccat
     5401 caccggctcg gcatcgggca tcggttttgc cagtgcccag gcgatgattg ccgccggcgc
     5461 gcaagtcgtg ctgattgacc gcgacgcacc ggccctggcc aaagcccgcg aagcattggg
     5521 cagcgccgcc ttgccgctgg tgctggacct gctggacccg gcgcagtgcg cggccatgcc
     5581 agcgcgcatt ctggctttgg ccggacggct cgacattttt cacgccaacg ccgggctgta
     5641 tgtgggtggc gatctgattg atgccaatga cgatgcgatc gaccgcatgc tcaacctgaa
     5701 catcaacgtg gtgatgaaga atgtgcgcgg cgtgctgccc cacatgattg agcggggcag
     5761 cggcgacatc atcgtcacca gctcgctcgc agcgcatttc cccaccccct gggagccggt
     5821 ctatgcatcc agcaagtggg ccatcgactg ttttgtgcag acggtgcgcc gccaggtgtt
     5881 caagcacggc atccgggtgg gtgccatctc gccagggcct gtcattaccg cgttgctggc
     5941 tgactggcct gccgaaaaac tggcagaagc caaggcccgg ggcagcttgc tcgaagcatc
     6001 agaggtcgcc aatgtcgtgc tgttcatgct cactcgtccg cgcggcatga cgatccgcga
     6061 tgtcgtgatg atgccctcgc agttcgacct ctgaaccggt gacccctggt gctactcatt
     6121 gacactcgct ctcaacaagg aaaccaaacc atgtacacga tccttcacct gggcctgggc
     6181 tcattccacc gtgcccacca ggctgtttac ctgcaccagt tgcagcagac cggcgaccga
     6241 agctgggctc tggcgggtgg caacatccgc cccgacatgg ccgacaccat agccgcactg
     6301 atcgcccagg gcggtgccta cacgctggaa accgtcaccc cgcataacca gcgcagctac
     6361 acccgcatca ccgccatcca gaccgtggtg ccgtttgatg ccgagctcag cggcctggtg
     6421 cgcatcggct cggatgccaa cacccggatc atctcgttca ccgtgaccga agccggttat
     6481 tacctcgatg ccaagtacca gctcgaccag aactttgccg acttgcgcgc cgacctggcc
     6541 gccgtcaagg cgggtgaacc ggtctgcacc atctacggtg ccctcaccgt gatcctgcgc
     6601 gcgcgcatgg ccgccaacgc cgggccggtg acgctgctca actgcgacaa cctgcgccac
     6661 aacggcgagc gctcacgcgg tggcctgctg cagttcattg agctgctcgg tgacagcgcc
     6721 ctgctgacct gggtaaacgc caacaccacc agccccaatg ccatggtgga ccgcatcacg
     6781 ccgcgcccga caccggcggt gcgcgagcgt gtgctggccg ccaccggcgt ggatgacgct
     6841 gccgcactga tgggcgaaag ctttatccag tgggtgattg aagacaactt catcgctggc
     6901 cgcccggcgt gggagtcggt gggcgtggag atggtcgatt cggtgcaggc ctacgaagaa
     6961 gccaagattc gattgctcaa tgccacccac agttgcatcg cctgggctgg tacgctggtg
     7021 ggctacacct acattcatga aggcacgctt gacccggtga tccgccagtt tgcctacgac
     7081 tacgtgaccg acgacaccat ccccgtgttg ctgccttgcc cgatcaacct ggagacctac
     7141 cgtgacgtgg tgctggaccg ctttggcaac cctgccattg ccgacaccaa ccagcgcgtg
     7201 gcgatggatg cgttttccaa gattcccgcc atgatcgcgc ccaccatccg cgaccggctg
     7261 gcacgtggcg agtcgatcaa tagcgtggcc atgttgccag cgctgttctt ggcttacctg
     7321 caacgctggc atgccgggca aattccttat acctaccagg atcaggcgat ggacccggcc
     7381 gtggcccacg ccatctgcga agccaccgac ccggtggccg cgttctgcgc cgacctcacc
     7441 ctgtggggcc cgctggcttg cgacgagcga ctcattgatg ccctgcgcca agccttcacc
     7501 cgagtcactc agttcgtcca gaaccatcaa aaatgaccgc agccagtggt aatgtaatgt
     7561 cctgatatct cgttattcat tcatcttgcg cccatgacca gcctgccacg tcaactcaag
     7621 cccgaactgg aacatgacta tgtccgctcg cctgatctgg gctatgagtc gcctgatgag
     7681 ccgggcttca tccgctgcct gtcgcatggt tttccgacac cgctggcgcg ctggcaccag
     7741 catgacgaat acgaactgca cctgatcacc gccacgtcgg gcaaggtgtt tgttggtgac
     7801 tggattggcc actttcaacc cggccatctg gtgctgactg gtccgcgctt gccgcacaac
     7861 tgggtcagca ccgatgtgcc agaaggtggc gtggccgagc gtgatctggt catccagttc
     7921 ctccatgcgc caatcgaaac cggcagccag cacataccgg aattgcgtga ggtgctacca
     7981 cttttggagc gggccaagca cggcatcgag ttttttggca tcttcccgca ggcactggcg
     8041 cactggcagc gcgtcaaggt tcaccagggg ctggcgcgac ttgccgcgtt ttgcgacttt
     8101 ttgagcgagt tgacccagtg caccgactac cggctgttgt cgagcaccca gatgcaaagc
     8161 gttgacaacg atgcccagtt ggagcaaatc aacgccatcg tcagccgcat caatgaacac
     8221 ctgtcagatc ccctgtcggc ggctgatctg gcgcgcgagc ttgacatgag cgaaagcgcc
     8281 ttttcacgtt ttttccgccg cgccaccggc aataccttca ccgactttgt caaccatgtg
     8341 cgggtcagcc gcgcatgcca gttgctgatg gagtcagact tgctgatcaa ccatattggc
     8401 taccaggtgg gcttcaacaa catcgccaat ttcaaccggc gttttctcga catcaagggc
     8461 atgacaccga gcgactaccg ccgtcaggcg gccagccgct ttggcctcaa ggcctgaaac
     8521 tttttttgac agcgagacat catgtacctc ggcattgatc tgggcacttc gggcctgaaa
     8581 ctcttgttgc ttgaccagca acaccatatc cacgccagcg ttgacgcagc catcacgctg
     8641 cagcgcccgc accccacctg gagcgaacag catccgcatg actggtggca ggcgctggag
     8701 tcagcggtgg cgcaactgcg cgccgccgca ccgcaagcct ggcgccaggt gcgtgccatc
     8761 ggcttgtctg gccagatgca tggtgcggtg gtgctggacg aaaacgccaa ggtgttgcgc
     8821 ccggccatcc tgtggaacga tggccgttcg cccgcacagt gcctggaact tgagcgtctg
     8881 gtgccgaact cgcgccagat caccggcaac ctggccatgc cagggtttac cgcgcccaag
     8941 ctgctgtggc tgcgcgaaca cgagccgacc gtctttgagc agatccgcac ggtgctattg
     9001 cccaaagatt ggctgcgcct gcaactcacc ggcgaggcag tcagtgagat gtcggacgct
     9061 tccggcacgc tgtggctcga tgttggcaaa cgctgctgga gcgagacgat gctggccgcc
     9121 tgtggcctgg acttgtcaca catgccgcgc ctggtggaag gcagccagcc cagcggatgc
     9181 ttgcagggcg cactggcgca gcgctggggc ctgactgcca gcgtggtggt ggccggtggc
     9241 ggcggcgaca acgccgccag cgcggtcggc attggggcgg ccaatcccgg ccagggtttt
     9301 gtgtcgttgg gtacttcggg agtggtgttt ttggtcagca atgtttacca gccggcacca
     9361 gagctagcgg tgcacgcctt tgcccatgca ctgcctgggc gctggcacca gatgtcggtg
     9421 atgctcagcg ctgccagcgc ctttggctgg gtcacgcaat tgaccggtag cccggatgaa
     9481 gcgcatctgg ccgaacaggt cgccgccttg ccgctgtcgc aacgggctgg cgccccgata
     9541 tttctgcctt acctgagcgg cgagcgcacc ccgcacaaca acccgaacgc cagcggtgtc
     9601 ttcatcggcc tgcgcggtga gcacgcagcc gctgacctgg gctatgcggt gatggagggg
     9661 gtcagttttg gactgatgga cggactgcgc gcgatgggtc aaaccgaggc ctcaactgtg
     9721 ccgctggccc tggtcggtgg cggtgcccgc agcgatgcct gggcgcaatt gctggccagc
     9781 ggtctggctt gtccgctgca ccggcccgag ggtgcgcacg ccgccgccgc tttgggcgca
     9841 gcgcgtttgg cctggctggc cgatggcggt accgagcaag aagtctgtca gccgctgccg
     9901 acggaacagg catttgcgcc ggagccgggc tgccgcgaac tgctggccga acgttacccg
     9961 cgctatcggg cgctttatcc ggcgctcagg gattttttct gaatttttag aacatttaga
    10021 tctggacagt gccaagttct ccccgttatt caggcggttt cttggccagg
//
LOCUS       DAOSLL010000090        10064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6508_length_10064_cov_4.706164, whole genome shotgun sequence.
ACCESSION   DAOSLL010000090 DAOSLL010000000
VERSION     DAOSLL010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10064
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6508_length_10064_cov_4.706164"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            13..117
                     /locus_tag="PLB25_05685"
                     /pseudo
     CDS             13..117
                     /locus_tag="PLB25_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008246911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
     gene            complement(160..1575)
                     /locus_tag="PLB25_05690"
     CDS             complement(160..1575)
                     /locus_tag="PLB25_05690"
                     /inference="COORDINATES: protein motif:HMM:NF033572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
                     /protein_id="HOE41105.1"
                     /translation="MKFQVEVVIKDESGQVMQRSVIFERLCVSFDDAIGGLGLSLADS
                     KTLVADVQKQRLETQVHGFSFGDKCCPTCSEAVKRKDVQTIVYRTLFGKFTLPNERFL
                     FCVSCGVQGKKSFSPLSDLLITHTHPELIYMQTRWAALLPFGQSLRLLEDVLPIEGAI
                     SLTNMKLKTAHVGQRIEADRTKQSELVAPPEIAQIAAAAGEEQTPTALDVGVDAGYIR
                     SNAAKGEDTRKFGAIAVKTVETKSRSHAYVQTEVADGDKRIIKFFEQGSDAPLTNVTF
                     FTDAGNDIKAATPLVGKGSQRILDWFHFAMYFQIALQTATPFKRWQYNAVRTIFEEIE
                     RIKWRFWHGQAEAACHRLQLLATWIGSKANTKVKVKVKLTERLFDLLHYAQDNIDYLV
                     NYARRHRNGQRISSAMAESVVNQVISRRFVKKQQMRWSPQGAHHLLQVRTAVLNDELA
                     QHFKRWHPGFATNDPVYARAA"
     gene            1883..2509
                     /locus_tag="PLB25_05695"
     CDS             1883..2509
                     /locus_tag="PLB25_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ANTAR domain-containing protein"
                     /protein_id="HOE41106.1"
                     /translation="MNKALRIVVVAPDLDSLDPGDDYNQNQAERSRSLRIGLLENGFN
                     LIASLPADVFLTERIAQLQPDMIIVDAESDARDALEHVVMATRDARRPIIMFTNDEDT
                     THVRAAVAAGVSAYVVAGLSTARIRPILDVALARFEHEQALLAELADVKSELKSRKII
                     DRAKGLLMQRLQLDEAAAYAKLRKTAMDKNLKLVEVAQRMLDMVDLLG"
     gene            2945..4189
                     /locus_tag="PLB25_05700"
     CDS             2945..4189
                     /locus_tag="PLB25_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmpA/NrtA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HOE41107.1"
                     /translation="MTDFLKPSLSRRAVLQTAAVGAIGISPALRTAVYAAGSDAPEKT
                     EVKIGFIPLTDCASVVMAAELGLDKKYGVKITPTKEASWAGVRDKLVTGELDFAHVLY
                     GLIYGVHLGVSGPKKDMAVLMTLNNNGQAITLSKKLADKGAVDGPSLARLMASEKREY
                     TFAQTFPTGTHAMWLYYWMAANGINPMKDAKVITVPPPQMVANMRVGNMDGYSVGEPW
                     NHRAIIDGIGITASTSQDVWKDHPEKVLGTTGDFVKKYPNTARAVTAAILEAGKWIDA
                     SLQNKNKMAETIAAKSYVNTSVDAINQRILGRYQNGLGKTWDDPNYMKFFNDGAVNFP
                     YLSDGMWFLTQHKRWGLLKEHPDYLAVAKQINQIEIYKQAAAASKTSLPKDLMRSSKL
                     MDGVVWDGKDPNKYADGFKIKV"
     gene            4263..5207
                     /gene="ntrB"
                     /locus_tag="PLB25_05705"
     CDS             4263..5207
                     /gene="ntrB"
                     /locus_tag="PLB25_05705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015414 - ABC-type nitrate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015706 - nitrate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate ABC transporter permease"
                     /protein_id="HOE41108.1"
                     /translation="MVSAVFHSPLETSIPSPVASSAAKIAMKKVAVSTDTARATGQKD
                     AKQNSHKPAYDWRGLWLAVLPPLFGLGLLIGVWALVSLGTSSSIPSPLATFKQAVVIF
                     SDPFYRNGPNDQGVGWNILMSLERVAVGFGLAAAVGIPAGFAIGRFEFLSRMFNPLIS
                     LLRPVSPLAWLPIGLLVFKGANPAAIWTIFICSIWPMIINTAVGVQRVPQDYMNVARV
                     LNLSEWKIVTKILFPSVLPYMLTGVRLAVGTAWLVIVAAEMLTGGVGIGFWVWDEWNN
                     LNVKNIIIAIFVIGIVGLILEYVLIKIATAFTFEEVKS"
     gene            5204..6040
                     /locus_tag="PLB25_05710"
     CDS             5204..6040
                     /locus_tag="PLB25_05710"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE41109.1"
                     /translation="MNENQTTKFIDISGVEQVFKTKNGPFCALQDIHLTVAKGEFVAL
                     IGHSGCGKSTLLNLIAGLTMPTQGHLLCDNREIAGPGPDRAVVFQNHSLLPWLTCFEN
                     VYLGVERVFGAASKSTGTASENKAQLTQRTHAALALVGLTPATQKRPGEISGGMKQRV
                     GIARALAMQPKVLLMDEPFGALDALTRAKLQDELLDIVSKTRATVVMVTHDVDEAVLL
                     SDKIVMMTNGPSATIGEVLSVKLARPRNRVELAEDRHYVQYRKAVIDFLYTRQSHVEK
                     AA"
     gene            6067..6954
                     /locus_tag="PLB25_05715"
     CDS             6067..6954
                     /locus_tag="PLB25_05715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41110.1"
                     /translation="MTNKTFIRCIEYWVPGDDGTVLEFGGGLFGPAKRFEAATRKLCF
                     GRGEGLPGQAWECGHPLMLKTLQAPSFRRAAAAQADGLSCAIALPLFADKRLCTVVVL
                     FCSDAVDLAGAIELWHNQPEESTDMTLLSGYYGNTGDTFEFLSSHTSFRRGTGLPGRT
                     WAGQAPVFMPDLGRGSGFLRADSALKVGINRGFAVPCGSTDGNIYVLAFLSALATPIA
                     SQVDVWQPDALQSHLVHSFSFAEAQPTAVAADDDKLALDDNIIAQVFATGVPSLLGQS
                     LVIPVAMQDRITAVLAISL"
     gene            7002..9530
                     /gene="nirB"
                     /locus_tag="PLB25_05720"
     CDS             7002..9530
                     /gene="nirB"
                     /locus_tag="PLB25_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0042128 - nitrate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase large subunit NirB"
                     /protein_id="HOE41111.1"
                     /translation="MKKMKLVMVGNGMAGVRTLEELLKIAPELYDITVFGAEPYPNYN
                     RILLSPVLAGEQTLDQIVLNDWAWYTDNHITLHAGWRVTSVDRARRLVHAHNAAGETL
                     SADYDRLLLCTGSNPFMLPVPGIDLKGVIAYRDIADTNAMIDAATTYQHAVVIGGGLL
                     GLEAANGLMLRGMKVSVVHVMPTLMERQLDAVAGKLLQKSLAERGLNFLMGAQTQALL
                     SPADLAGASADAEFQAAAACVPDLQAKLASSPDRVCAVSFKDGSVVAADLVVMAVGIR
                     PNVELAQSMRLHCDKGIVVNDTLQTTTDARIYSVGECAAHRGTAYGLVAPLFEQAKVA
                     ANHLAQFGIGRYVGSLTSTKLKVTGIDLFSAGNFVGGEGFEEIILSDPKGGVYKKIVL
                     KDDKLVGACLYGDTVDGSWYFKLLRDGRPVADIRDRLMFGESNIGDAGHQGQNKAAAM
                     QDSDEVCGCNGVTKGTICKVIKEKGLFTLDEVRKHTKASASCGSCTGLVEQLLIFTAG
                     ADYSATPKTKAMCACTEHGHQAVREAIKGNKLLSIADVFAFMEWKTPNGCASCRPAVN
                     YYLISTWPKEAKDDPQSRFINERSHANIQKDGTYSVIPRMWGGETNASELRRIADVVD
                     KYKIPTVKVTGGQRIDLLGVKKEDLQAVWQEIGMPSGHAYAKALRTVKTCVGSEWCRM
                     GTQDSTQMGKDLERAMWRMYAPHKVKFAVSGCPRNCAEAGIKDVGIIGVDSGWEMYVA
                     GNGGIKTEVAHFLVKLKTAAEVLEYTGAFCELYRSEGWYLERTVHYVNRVGLDYVKKR
                     ILEDHAGRLALWAQLQFALDGEPDPWFDFDKAAVDTRQFAALTV"
     gene            9709..>10064
                     /gene="nirD"
                     /locus_tag="PLB25_05725"
     CDS             9709..>10064
                     /gene="nirD"
                     /locus_tag="PLB25_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HOE41112.1"
                     /translation="MTDWTLICQLEDIPVLGARRVKRSAGMDVAVFRNDKDELFALLD
                     RCPHKGGPLSQGIVFGSSVACPLHNWTIGLCSGQAVAPDEGCTPKFSVKLERGQVFLM
                     TAELASHATEQIRPVAG"
BASE COUNT         2191 a         2826 c         2909 g         2138 t
ORIGIN      
        1 cttaactttt ctttgctgca aactctcgtg gaaattctga ccacgctggc ctttgcgctt
       61 tccggcatga tggaggcagc gcgaaagcgg ctcgatatgg tgggcgtttg tgtggtggta
      121 ggcttgaggg actcgcccaa accaagggaa agtcatattt caggcggctc tagcgtagac
      181 gggatcattc gttgcaaagc ctggatgcca gcgtttgaaa tgctgcgcga gctcatcgtt
      241 gagcacagcg gttcgcactt gaagcagatg gtgggctccc tggggtgacc aacgcatttg
      301 ctgcttcttc acaaagcgtc ggctgatgac ttggttgacc acagactcgg ccatggccga
      361 cgatatgcgt tgcccattgc ggtgtcgccg tgcgtagttc accagatagt ctatgttgtc
      421 ctgggcgtaa tgaagcagat cgaatagccg ctctgtcagt ttcactttga ccttgacctt
      481 ggtgtttgcc tttgagccaa tccaagtggc aagcagttgc agccggtgac atgccgcctc
      541 tgcctgacca tgccagaacc tccacttgat acgttcaatt tcttcgaaga tggttcgcac
      601 cgcgttgtat tgccagcgtt tgaatggtgt ggccgtttgg agtgcaattt gaaagtacat
      661 cgcaaagtga aaccaatcca aaatccgctg gctgcctttg cccacaagcg gcgtggccgc
      721 cttgatatcg ttaccggcat cagtgaagaa cgttacgttg gtcagcgggg cgtcgcttcc
      781 ttgctcgaaa aatttgataa tacgcttgtc tccatcagcg acctccgtct gaacgtaggc
      841 atggctgcgg ctcttggtct caaccgtttt caccgcgatc gcgccgaatt tgcgagtgtc
      901 ctcgcccttg gctgcgttgg agcggatgta gcctgcgtca acacccacgt ccagtgcggt
      961 gggggtttgt tcctcgccag ccgccgcggc tatttgcgca atctcaggtg gcgctaccaa
     1021 ttcggactgc ttggtgcgat cagcttcaat gcgttgcccg acatgtgccg ttttgagctt
     1081 catgttggtc aaactgatcg ctccttcaat gggcagcacg tcttcgagca gtcgcagcga
     1141 ctgcccgaat ggcagtagag cagcccagcg tgtctgcata tagataagct cagggtgcgt
     1201 atgcgtgatc agcagatcag acagtgggct gaacgacttc ttgccttgaa cgccacaaga
     1261 gacgcagaag agaaaacgct cattgggcaa agtgaactta ccaaacagtg tgcggtaaac
     1321 gatggtctgc acatccttgc gcttcaccgc ttcgctacat gttgggcaac atttatctcc
     1381 gaacgagaac ccatgtactt gagtttcgag ccgctgcttt tgaacgtctg cgacaagggt
     1441 tttgctatcc gcaagactca gcccaagccc gcctattgcg tcatcaaaag aaacacaaag
     1501 cctctcaaaa attacagatc gctgcatcac ctggcccgac tcatctttga taaccacttc
     1561 gacttggaat ttcatttgcc gccttcagac tgaacatgtc tggatttgac aacaatcagg
     1621 attcagcacc agatttactg ggtttccctt ggtttgggcc agtcccagca aaacggtggg
     1681 ggtggaacca cgaattggga tgatgttgat gtgcatgtct acattataca aaatattatc
     1741 gattaaatta agctcaatac atgtctacat atttgcactc aatataccac taacgtgttg
     1801 atttcaaaag gaattcaacc aaatccggat ggaacttcag tcgaggggga gcattgcggc
     1861 gtgaatcttg ctttttggat ccatgaacaa agcactgcgc atcgttgttg tcgcgcccga
     1921 tcttgatagc ctcgatccgg gtgatgacta caaccaaaat caagctgagc gttcgcgcag
     1981 tctgcgtatc ggcttgctgg aaaacggttt caacctgatc gccagcctgc ctgccgatgt
     2041 gtttctgacc gaacggattg cccagttgca acccgacatg atcattgtcg atgccgagag
     2101 cgacgcgcgt gatgcgcttg aacacgtggt gatggccacc cgcgatgcgc gccgcccgat
     2161 catcatgttc accaacgacg aagacaccac gcatgtccgc gcggccgtgg cggctggcgt
     2221 gtcggcctat gtggtggccg gtttgtcaac tgcacgcatc cgcccaattc tggatgtggc
     2281 cttggcccgc tttgagcacg aacaggccct gctagctgag ctggcggatg tgaaaagcga
     2341 actcaaaagc cgcaaaatca ttgatcgggc caaaggtctg ctgatgcagc gcctgcagct
     2401 tgacgaagca gcggcctacg ccaaactgcg caaaactgcg atggacaaaa acctgaaact
     2461 ggtggaggtg gcgcagcgca tgctcgacat ggtggatttg ctcggctagc acaaaaatgg
     2521 tgaaaaaacc agaccgtgcc agcatcagcc tgacctaccg tggcccggcc tgcatgcccg
     2581 aattggtgca acgcacaaca atggggttga cctgtcaatc caacaaaaaa gtgtactgaa
     2641 tatacctcta gcccttactg cataagcgta agcggctata tttagtgtag caatatgcgg
     2701 gttgattcat actggcgcgt ttattgcttg tatgttagca agacccgcaa gggttgcacg
     2761 aaagttccag tccaacggcg ggctgcgaac atcgtcaccg gacaaaggcg tccccatttg
     2821 gcaaacagct tctttggaac tgttttgtta cgtgaggtcg cctttttttt cgttctgaaa
     2881 cttcgctgga ctgtcctggg tgctgtgcgc cggggtcagg ttcgcgtcac ctggggaaat
     2941 tgtcatgact gattttctga aacctagcct gagtcgccgc gctgttctgc aaaccgctgc
     3001 cgtcggtgcc atcggcatca gtccggcact gcgcactgcg gtctatgccg caggctctga
     3061 tgcgccggaa aaaaccgagg tcaaaatcgg ctttatcccg ctgaccgact gcgccagcgt
     3121 ggtgatggcg gcagaactcg ggctggacaa aaaatacggg gtcaagatca ccccgaccaa
     3181 ggaagccagt tgggccgggg tgcgcgacaa gctggttacc ggcgaactgg actttgccca
     3241 tgtgctgtac ggcctgattt acggcgtgca tctgggggtg agtggcccga aaaaagacat
     3301 ggcggtgctg atgacgctca acaacaatgg ccaggccatc acgttgagca aaaagctggc
     3361 cgacaaaggc gcagtcgatg gcccgagcct ggcccggctg atggcttctg agaagcgcga
     3421 atacaccttt gcccagacct tcccgaccgg cacccacgcc atgtggctgt attactggat
     3481 ggccgccaac ggcatcaacc cgatgaaaga tgccaaggtc atcaccgtgc cgccaccgca
     3541 aatggtggcc aatatgcggg tgggcaatat ggacggctac agcgtcggcg aaccctggaa
     3601 ccaccgcgcc atcattgacg gcattggcat caccgccagc acctcgcaag atgtctggaa
     3661 agaccacccg gaaaaagtgc ttggcaccac cggcgacttt gtcaagaaat accccaacac
     3721 ggcgcgtgct gtcaccgccg cgattctgga agctggcaag tggattgacg cgagcttgca
     3781 aaacaaaaac aagatggctg aaaccattgc cgccaaaagc tatgtcaaca ccagcgtgga
     3841 tgccatcaat cagcgcattt tgggccgcta ccaaaatggc ctgggcaaaa cctgggacga
     3901 ccccaattac atgaagtttt tcaacgacgg cgcggtcaac ttcccctatc tttctgacgg
     3961 gatgtggttt ttgacccaac acaagcgctg gggtctgtta aaggagcacc ccgattacct
     4021 ggcggtggcc aaacagatca accagatcga gatttacaag caagccgctg ctgccagcaa
     4081 aacgtcgctg ccgaaggacc tgatgcgcag cagcaaactg atggacggcg tggtgtggga
     4141 cggcaaagac ccaaacaaat acgctgacgg tttcaagatc aaggtctgaa acatggcatc
     4201 gctggccgcc gtcacacgcc agcgtaccag ctctgcttgc aacgcatcaa aaaggaaaca
     4261 ccatggtcag cgccgttttt cactcgccac ttgaaacctc aatcccctcg cctgtggctt
     4321 cgtcagccgc aaaaattgca atgaaaaaag tagctgtcag cacagacaca gcaagggcta
     4381 caggtcaaaa agatgcaaaa caaaatagcc acaaaccagc ttatgactgg cgcggtctgt
     4441 ggctggcagt gctgccgccg ctgtttggtc tggggctgct gatcggggtc tgggcgctgg
     4501 tgtctctcgg cacttccagc agcatcccgt cgccgctggc caccttcaag caggcagtgg
     4561 tcattttcag cgacccgttt taccgcaatg gccctaacga tcagggtgtc ggctggaaca
     4621 ttttgatgag cctggaacgg gtggcggtcg gctttgggct ggcggcggcg gtcggcattc
     4681 cggccggatt tgcgattggc cggtttgaat ttttaagccg catgttcaac ccgctgatca
     4741 gcctgctgcg cccggtgtcg cccttggcct ggctgccgat tggcctgctg gtgttcaagg
     4801 gggccaaccc ggcagcgatc tggacgattt tcatctgctc gatctggccg atgatcatca
     4861 acaccgctgt cggcgtgcag cgggtgccgc aggattacat gaacgtggcc cgcgtgctga
     4921 atttgagcga gtggaagatc gtcaccaaaa ttttgtttcc ctcggtgctg ccttacatgc
     4981 tgactggtgt gcggctggcc gtgggcaccg cctggctggt gattgtggcg gccgagatgc
     5041 tgaccggcgg tgtcggcatc ggtttctggg tctgggacga gtggaataac ctgaatgtca
     5101 aaaacatcat catcgccatc tttgtgatcg gcatcgttgg cctgattctg gaatatgtcc
     5161 tgatcaaaat tgccaccgcc ttcacctttg aagaggtgaa gtcgtgaacg aaaaccaaac
     5221 caccaagttc atcgacatca gtggtgtcga gcaagttttc aaaaccaaaa atggcccgtt
     5281 ttgcgccttg caagacattc acctgacagt tgccaagggt gaatttgtcg ccctgatcgg
     5341 tcactcgggt tgcggcaaat cgactctgct gaacctgatc gccgggctga ccatgccgac
     5401 ccagggccac ctgctgtgcg acaaccgtga aatcgccggc cccggcccgg atcgtgccgt
     5461 ggtgtttcaa aaccactcgc tgctgccctg gctgacctgc ttcgagaatg tttaccttgg
     5521 tgtcgaacgg gtgtttggtg cggccagcaa atctaccggt accgcgtccg agaacaaggc
     5581 gcaactgacc cagcgtaccc acgccgcgct ggcgctggtg ggtttgaccc cggcgacaca
     5641 aaaacgcccc ggcgagattt cgggcggcat gaagcagcgt gtcggcattg cccgtgcctt
     5701 ggcgatgcag cccaaggtgc tactgatgga cgagccgttt ggcgcgcttg acgcactgac
     5761 ccgtgccaag ctgcaagacg agttgctcga cattgtgagc aaaacccggg ccacggtggt
     5821 gatggtgacc cacgatgtgg atgaagccgt actgctcagc gacaaaattg tgatgatgac
     5881 caacggcccg tcagccacca tcggcgaagt gctgtcggtc aagctggcgc gaccgcgcaa
     5941 ccgggtcgaa ttggccgaag accgccacta tgtgcagtac cgcaaggcgg tgattgactt
     6001 tttgtacacc cgacaaagtc atgtcgaaaa ggccgcctga gccacatgac cagccaagga
     6061 caccccatga ccaataaaac ctttatccgc tgcattgaat actgggtgcc cggcgacgat
     6121 ggcaccgtgc tggagtttgg cggtggcctg tttggcccgg ccaaacgctt tgaggccgcc
     6181 acccgcaagc tgtgctttgg tcgtggcgag ggtctgcccg gccaggcctg ggaatgcggt
     6241 cacccgctga tgctcaaaac ccttcaagcc ccaagttttc gccgtgctgc ggcagcgcag
     6301 gcggacggcc tgagttgtgc cattgcgctg ccgctgtttg cggataagcg cctgtgcact
     6361 gtggtggtgc tgttttgcag cgatgcggtt gacctggccg gagccattga gctttggcac
     6421 aaccagcccg aagagtcgac cgacatgacg ctgctcagcg gctactacgg caacaccggc
     6481 gacacctttg aattcctctc cagccacacc agttttcggc gcggcaccgg cctgccgggt
     6541 cgcacctggg ccgggcaggc cccggtgttc atgcccgacc tgggccgtgg ttcgggcttt
     6601 ttgcgtgctg acagcgccct aaaagtgggc atcaaccggg gttttgcggt gccttgcggt
     6661 tcgaccgacg gcaatatcta tgtgctggca tttttgagtg cactggccac cccaattgcc
     6721 agccaggtcg atgtctggca gcccgatgcc ctgcagtcgc acctggttca cagcttcagt
     6781 ttcgccgaag cacagccgac tgcggtagcc gccgatgacg acaaactagc gcttgacgac
     6841 aacatcatcg cgcaagtatt tgccaccggc gtgccgagcc tgctgggcca gagcctggtg
     6901 attccggtgg cgatgcaaga ccgcatcacc gccgtgctgg ccatatcttt gtgatgtcct
     6961 tgtaatggtg ttgtgcgctg caattgagtt tggagaatcc gatgaaaaaa atgaaacttg
     7021 tgatggtcgg caacggcatg gcaggtgtgc gcacccttga agagttgctc aaaattgcgc
     7081 ccgagttgta tgacatcacg gtgtttgggg ccgagccgta tcccaactac aaccgcattt
     7141 tgctgtcgcc ggtgctggct ggcgagcaaa ccctggacca aatcgttctc aacgactggg
     7201 cctggtacac cgacaaccac atcaccctgc atgctggctg gcgcgtgact tcggttgacc
     7261 gagcccggcg gctggtgcat gcccacaatg cggccggcga gacgcttagc gctgactatg
     7321 accgcctgct gctgtgtacc ggctccaacc cgttcatgct gccggtgccc ggcattgacc
     7381 tcaaaggtgt gatcgcatac cgcgacattg ccgacaccaa cgcgatgatc gatgctgcca
     7441 ccacctacca gcacgcggtg gtcattggtg gcggcctgct cggcctggaa gccgccaacg
     7501 gcctgatgct gcgtggcatg aaagtcagcg tggtgcatgt gatgccgacg ctgatggagc
     7561 gccaattgga cgcagtggca ggcaagctgc tgcaaaaatc gctagcagaa cgtggcctga
     7621 attttttgat gggagcgcaa acccaggcgc tgctcagccc ggccgaccta gccggtgcca
     7681 gtgccgatgc agagttccag gcggctgccg cttgtgtgcc ggatttgcaa gcaaaattgg
     7741 cctctagtcc agaccgggtc tgcgcagtga gcttcaaaga tggtagcgtt gtagcagccg
     7801 atctggtggt catggcagtc ggtattcggc ccaacgtcga actggcgcaa agcatgcggc
     7861 tgcattgcga taagggcatt gtggtcaacg acacgctgca aaccaccacc gatgcgcgga
     7921 tttactcagt cggtgaatgc gccgcccacc ggggtaccgc ttatggtctg gtggccccat
     7981 tgtttgagca ggccaaggtg gcggccaacc atctggcgca gtttggcatt ggccgctatg
     8041 ttgggtcact gacctcaacc aagctcaagg tcaccggcat cgacctgttt tctgctggca
     8101 actttgttgg cggcgaaggc tttgaggaaa tcatcctgag cgaccccaag ggcggggttt
     8161 acaaaaaaat tgttctcaag gacgacaagc tggttggtgc ctgcctgtat ggcgacacgg
     8221 tcgatggcag ttggtatttc aaactgctgc gtgatgggcg acccgttgcc gacatccgcg
     8281 atcggctgat gtttggcgaa agcaacatcg gcgatgccgg tcaccagggc cagaacaagg
     8341 ccgcagccat gcaagacagc gacgaggtgt gcggctgcaa tggtgtcacc aagggcacca
     8401 tctgcaaggt gatcaaggaa aaaggcctgt tcaccctgga cgaagtgcgc aaacacacca
     8461 aggccagcgc gtcctgcggc tcctgcaccg ggctggtcga gcaactgctg atttttaccg
     8521 ccggtgccga ctactcggcc acgcccaaaa ccaaggccat gtgcgcctgt accgagcacg
     8581 gtcaccaggc ggtgcgcgag gccatcaaag gcaacaagct gctcagcatt gctgatgtgt
     8641 ttgcctttat ggagtggaaa acccccaacg gttgcgccag ttgccgcccg gcggtcaact
     8701 attacctgat cagcacctgg ccgaaagagg ccaaagacga cccgcaaagc cgctttatca
     8761 acgaacgcag ccatgccaat atccagaaag acggcactta cagcgtgatt ccgcgcatgt
     8821 ggggcggcga aaccaacgcc agcgagctgc gccgcattgc cgacgtggtg gataaataca
     8881 aaattccgac ggtcaaggtc actggtggcc agcggattga cctgctgggg gtcaaaaaag
     8941 aagacctgca agcggtctgg caagagattg gcatgcccag cggccacgcc tacgccaaag
     9001 cgctgcgcac ggtcaaaacc tgcgtcggca gcgaatggtg ccgcatgggc acgcaagaca
     9061 gcacccagat gggcaaggat cttgagcgcg ccatgtggcg aatgtacgcg ccgcacaagg
     9121 tcaagtttgc cgtatcggga tgcccgcgca actgtgccga ggctggcatc aaggatgtcg
     9181 gcatcatcgg ggtcgatagc ggctgggaaa tgtatgtggc cggcaacggc ggcatcaaga
     9241 ccgaagtggc gcactttctg gtcaagctca aaaccgccgc cgaagtgctg gaatacaccg
     9301 gcgcattttg cgagctgtac cgcagcgagg gctggtatct ggagcgcacc gtgcactatg
     9361 tgaaccgggt cggcctcgat tacgtcaaaa agcgcattct cgaagaccat gcgggtcgct
     9421 tggcgctttg ggcgcagttg cagtttgcac ttgatggcga gcctgacccg tggtttgact
     9481 ttgacaaggc ggcggtcgat acacgccagt ttgcagccct gacggtttaa ctgcattcaa
     9541 tccggcattg aatgcttttt atggctctag cccatgtcag ataagcgcaa gcagctatta
     9601 aaaacatagt cttcaatccg ctgctggcca agttaacctg atttatgaga ctttttggcc
     9661 ttcagcccat acacagcctg cgcaagcagc tatacaagga atagcaagat gaccgattgg
     9721 acgctgattt gccagcttga agacatcccc gttctgggcg cacggcgcgt caagcgcagc
     9781 gctggcatgg atgtcgcggt gtttcgcaac gacaaggacg agctgtttgc cctgctcgac
     9841 cgctgcccgc acaagggtgg cccgttaagc cagggtatcg tgtttggcag cagcgtcgcc
     9901 tgtccgctgc acaactggac gattggcctg tgcagcggcc aggcggtggc accggatgaa
     9961 ggctgcacac caaaattcag cgtcaagctc gaacgtggcc aagtgttcct gatgactgca
    10021 gaactggcca gtcacgccac cgaacaaatc cgtccggtcg cggg
//
LOCUS       DAOSLL010000091         9955 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6569_length_9955_cov_4.549899, whole genome shotgun sequence.
ACCESSION   DAOSLL010000091 DAOSLL010000000
VERSION     DAOSLL010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9955
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6569_length_9955_cov_4.549899"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /gene="fbaA"
                     /locus_tag="PLB25_05730"
     CDS             <1..646
                     /gene="fbaA"
                     /locus_tag="PLB25_05730"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOE41113.1"
                     /translation="SEEPIQENIEICSRYLERMSKMGMTLEIELGVTGGEEDGVDNSS
                     VDSSKLYTQPEDVAFAYEALSKVSDRFTIAAAFGNVHGVYKPGNVKLQPIILKNSQDY
                     VQKKFSTKPGPVNFVFHGGSGSTLEEIREAIGYGVVKMNIDTDLQWAFWDGVRAFYKK
                     NEGYLQGQIGNPEGDDKPNKKFYDPRAWMRKGEDAFRARLKQAFTDLNNIGTLA"
     gene            complement(761..934)
                     /locus_tag="PLB25_05735"
     CDS             complement(761..934)
                     /locus_tag="PLB25_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41114.1"
                     /translation="MNDEIYQKSEINGRCALASSHAQASTGAQFVALVEAAQIIGANR
                     RNQVHQGFNRFSA"
     gene            1020..1841
                     /locus_tag="PLB25_05740"
     CDS             1020..1841
                     /locus_tag="PLB25_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE41115.1"
                     /translation="MKLDMISRYPATTAKRVPLLFLHGSFSDARVWSDHFLPYFAEHG
                     YEAHAFSLRGHGRSEGLERIATWRLADYVADLEKVAASFSCAPIMIGHSMGGMVIQKY
                     LEKHRHASGLVLMASVPPQGLMATTMHMAMRHPFLFQQMAQLSMLGPRFGTPMIMRKI
                     LFSRDMPESKLLGLFNYMQAESQVVSMDMMSSTALQLKPETLRMPVLVLGAHNDVLVA
                     PTFVEQTARFYKAELHIFRDMGHAMMLDTHWIKVADCLLAWLERITAAKSANVEG"
     gene            complement(2200..>2417)
                     /locus_tag="PLB25_05745"
     CDS             complement(2200..>2417)
                     /locus_tag="PLB25_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41116.1"
                     /translation="DKGQRTKDKGQRTKDKGQRVCCKGQSHHDSQGLLIAATMSESDP
                     GRRATGSAQKAVLKSNRVQIQMSPADT"
     assembly_gap    2418..2517
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2838..4463
                     /gene="ipdC"
                     /locus_tag="PLB25_05750"
     CDS             2838..4463
                     /gene="ipdC"
                     /locus_tag="PLB25_05750"
                     /EC_number="4.1.1.43"
                     /EC_number="4.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate/phenylpyruvate decarboxylase"
                     /protein_id="HOE41117.1"
                     /translation="MNLTESLLHALKKHGARQIFGIPGDFALPYFRIIEDSKILPLYT
                     LSHEPGVGFAADAAARINAGLGVAAVTYGAGALNMINSVAAAFAEKSPLVVLSGGPGK
                     GESRSGLLLHHQAKTLDSQFHIFKEITCDQVRLDDAARAPTDIARVLASCLRHSEPVY
                     IEIPRDMVAVTCAEVVPETPQPVDQDALNACVDEILERLGRASSPVLMAGVEVRRYGL
                     EDKVAALSRRLGLPVVTSFMGRGLLSGQDAPLVGTYMGVAGFPEVTQLVEGSDGLFLL
                     GEIICDTNFAVSETKIDMRKTIQALNGQVNIGYHTYDKLPLAALVDRMLQRVGSVDKA
                     FDITPQVFPRHLQADASSINPTDIATAVNDLMAEHGKMPIASDMGDCLFTAMEIEHTA
                     LVAPGYYATMGFGVPAGLGLQAATGERPLILVGDGAFQMTGWELGNCKRYGWDPIVLL
                     FNNASWEMLRTFQPESSFNDLGHWGFAEMASGLGGDGVRVGSRAELQAALAKAIVTRG
                     KFQLIEITIPRGVLSATLARFVAGVKRLNAVKN"
     gene            4502..5215
                     /gene="serB"
                     /locus_tag="PLB25_05755"
     CDS             4502..5215
                     /gene="serB"
                     /locus_tag="PLB25_05755"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HOE41118.1"
                     /translation="MSHPLEAADLVIKGIRPPLKLSDFKLIAFDMDSTLINIECVDEI
                     ADAAGRKAEVAAITEAAMQGEIADYKDSLRRRVRLLKGVTTVQMDLVLHERLQLNPGA
                     ATLVAACQRAGLKVLLVSGGFTYFTDHVRDLLAIDYTRSNVLEVVDAKLTGRLLDQPW
                     GDICDGDEKRKMLLATCAQLGLSPLQSIAMGDGANDIPMMSAAGLAVAYHAKPTVRDF
                     AQVCINAGGLDQLLQVFAP"
     regulatory      5688..5784
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5824..6654
                     /gene="thiM"
                     /locus_tag="PLB25_05760"
     CDS             5824..6654
                     /gene="thiM"
                     /locus_tag="PLB25_05760"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HOE41119.1"
                     /translation="MSHPILPADLWADVLSVRSVRPLVHSITNLVVMNFNANVLLAMG
                     ASPVMAHAHEEVADMASIAQALVLNIGTLEPYWIESMRKALEIASKKGIKTVLDPVGA
                     GATSYRNDSIKVLLKQAMPSVIRGNASEIMSVAGSAAQTRGVDSSAAVGDALQCARAL
                     ALQTGGVVCVSGPVDHVFDADGRHASLSNGHEWMTRITGVGCSASALVGAFCAVQPDA
                     WRATLSAMAYLGVVGEVAAQAVQQRNQGVGSLQIALLDQLQLLDQASFERQLRMAVSS
                     "
     gene            6651..7331
                     /gene="thiE"
                     /locus_tag="PLB25_05765"
     CDS             6651..7331
                     /gene="thiE"
                     /locus_tag="PLB25_05765"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HOE41120.1"
                     /translation="MIVHSDVADATARATLTRALRLYLVTDQASLRGRALADVVLAAL
                     KGGVTCVQLREKSLCTRDFVAMGLAMQDLLAAFAVPLIINDRIDVALACGAQGVHLGQ
                     SDMPVAVARRLLPPQVFIGLSVETMDDVANAAMQPVDYLGVSPIYPTPTKTDTAPPWG
                     LDGLRQVRAATALPLVAIGGIQLAQAGEVLQAGADGLAVVSAICSADDPEAAARLIFA
                     QLRSQNAR"
     gene            complement(7471..9330)
                     /gene="ilvD"
                     /locus_tag="PLB25_05770"
     CDS             complement(7471..9330)
                     /gene="ilvD"
                     /locus_tag="PLB25_05770"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013952014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOE41121.1"
                     /translation="MPVYRSKTTTAGRNMAGARSLWRATGMKDDDFDKPIIAVVNSFT
                     QFVPGHVHLKDLGQLVAREIEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRDIIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKTRLLNPAT
                     QKLEFKKLDLIDAMVMAADDQVSDADVAEVERSACPTCGSCSGMFTANSMNCLTEALG
                     LALPGNGTVVATHADREQLFKRAGHLAVELCQRYYEQDDTTVLPRSMGFKAFENAITL
                     DIAMGGSTNTILHILAIAQEAEIDFTLADIDRLSRSVPQLCKVAPNTNKYHIEDVHRA
                     GGIYGILGELDRAGKLHTDVPTVHSKTFKEALDRWDIARNPSDAVKTFYMAGPGGIPT
                     QVAFSQSARWPSLDLDRAEGCIRSGEHAFSKEGGLAVLRGNIALDGCVVKTAGVDDSL
                     MVFEGPAHITESQDEAVEHILADQVKAGDVVIVRYEGPKGGPGMQEMLYPTSYIKSKG
                     LGKACALLTDGRFSGGTSGLSIGHASPEAAAGGAIGLVRNGDRIRIDIPGRSINVLVS
                     DDELARRRAEQDALGWKPAQPRKRKVSAALRAYAKLVMSADKGAVRDLSLLED"
     gene            complement(9456..9704)
                     /locus_tag="PLB25_05775"
     CDS             complement(9456..9704)
                     /locus_tag="PLB25_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04438 family Trp-rich protein"
                     /protein_id="HOE41122.1"
                     /translation="MYLLGLGLALLVLKYIEWAPVTNWSWWVVMTPFGLAVAWWAWAD
                     STGYTKRKAMAQEEKRRDERRERTKQALDANYRKRPRG"
BASE COUNT         1982 a         2919 c         2865 g         2089 t
ORIGIN      
        1 gtcggaagag ccaattcagg aaaatatcga aatttgcagc cgttaccttg agcgcatgag
       61 caagatgggc atgacccttg aaatcgaact cggcgtaacc ggtggcgaag aagacggcgt
      121 tgacaactcc agcgttgaca gctccaagct ctatacccag cccgaagacg ttgcttttgc
      181 ctacgaagca ctgtccaagg tcagcgaccg cttcaccatc gccgccgcct ttggcaatgt
      241 gcatggcgtg tacaagccag gcaacgtgaa actgcagccg atcatcctga agaattcgca
      301 ggactacgtg caaaagaaat tcagcaccaa gcccggcccg gtcaactttg tgtttcatgg
      361 cggctcgggc tcgaccctgg aagaaatccg cgaagcaatc ggctacggtg tcgtcaagat
      421 gaacatcgac accgacctgc aatgggcctt ctgggatggc gtgcgcgctt tctacaagaa
      481 aaatgaaggt tatctgcaag gccagatcgg caaccccgag ggtgacgaca agcccaacaa
      541 gaagttctac gacccacgcg catggatgcg caagggcgaa gacgctttcc gtgccagact
      601 gaaacaagcc tttaccgatc tgaacaacat cggcacgctg gcctaaggga gattgagggt
      661 agttgggtcg atgtcccggt gcgcttttgc cctggcatcg accccgcact gaggcgggac
      721 gcacctgttt ctgtcgggtt tcttgctggt gcaattcact tcaagcgctg aatctgttga
      781 aaccctgatg tacctggttg cgccggtttg cgccgatgat ctgcgcagct tcaaccaatg
      841 caacaaactg cgcgcctgtc gatgcttgag catgacttga tgcgagggcg catcgcccgt
      901 tgatttcact tttttgatag atttcatcat tcacatgggc agcagcacat caaaggccca
      961 atcaagtcca tcctgtacgc caggttgccg cgttttttaa ccacataaga tttcctgtta
     1021 tgaaattgga catgatcagc cgctatcctg cgacgactgc caagcgagtg ccgctgttgt
     1081 tcctgcatgg ctctttttcg gatgcacgcg tctggagcga tcacttttta ccctacttcg
     1141 ccgagcatgg ctatgaagcc catgccttca gtctgcgcgg tcatggccgc agcgaaggtc
     1201 tcgagcgcat cgcgacctgg cggctggctg actatgtcgc cgaccttgaa aaagtcgccg
     1261 caagcttttc ctgtgcgccc atcatgattg gtcattcgat gggtggcatg gtgattcaga
     1321 agtatctgga aaagcatcgc cacgcgtccg gactggtgtt gatggcttcg gtgccaccgc
     1381 agggtctgat ggcaaccacc atgcacatgg cgatgcgtca tccgttcctg ttccagcaaa
     1441 tggcccagct ttccatgctc ggtcccagat ttggcacgcc aatgatcatg cgcaagatct
     1501 tgttttccag ggacatgcca gagagcaaat tgctcggttt gttcaactac atgcaagccg
     1561 aatcgcaggt ggtgtcgatg gacatgatgt cgtcaacagc gctgcagctc aaacccgaaa
     1621 ccttgcgcat gccagttctg gtgctcggcg cccacaacga tgtactcgtg gcacccacat
     1681 ttgtcgagca aaccgcccgc ttctacaagg ccgagttgca catcttccgc gacatgggtc
     1741 acgccatgat gcttgacacg cactggatca aggtagcaga ctgcctgttg gcctggctgg
     1801 agcgaatcac ggcagccaag tcggcaaacg tcgaaggctg agcgcccgtc atcgcgattt
     1861 tttgcggcgg ctggctgctc cgctgcttgc cgggttcatg aacttgctct ggacttcatc
     1921 gctcctcaaa ccgttgtcag gcaagccacg ggtacagtga atctgggacg aatccgactt
     1981 tgcggccatc agagcgaacc aggacccagg tcgcacagag aaatagggct ctgcgcacac
     2041 tttcatgggc caggtgtggc gctgtcaatt cgctacgggg ggcccaagca tcgtagcatt
     2101 tgctttgaat tctccgtttc aatttcagcc acagtttcat gagtatttgg cctgcagccc
     2161 ccgtggatta tgcgctgaaa gctatttttt ttatagtaat caggtatcgg cgggtgacat
     2221 ttggatctga acccgattgc tctttaagac tgctttctga gctgaaccag tggcgcgacg
     2281 accggggtcg gactccgaca ttgttgcagc gatcaacaga ccttgactat cgtggtgcga
     2341 ctggcccttg caacaaaccc tctgtccttt gtcctttgtc ctttgtcctt tgtcctttgt
     2401 cctttgtcct ttgtcctnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnncct
     2521 ttgtcctttg tcctttgtcc tttgtccttt gtcctttgtc ctttgtcctt tgcagtcaag
     2581 catggcgtgg cttcgatcca agacacattt gctctttcgc taagtcgttg atttaccgtc
     2641 attcccgcgc aggcgggatt gacgacgatg ccaaaagaac aaacttaccc taggttctat
     2701 cgaacacggc attgccagtc acaaagaggg gtctgcatca tgccctgctg tgacggatgc
     2761 aacacgggtt ggcagcggcg gctagccaac cagcgaacgc gataatcccg ccaccccttg
     2821 ttttggagcc attacccatg aacctcaccg aatcgctgct gcacgcgctc aaaaagcacg
     2881 gtgcccggca aatctttggc atcccgggcg actttgccct gccctacttc cgcatcattg
     2941 aagactcaaa aatcctgccg ctctacaccc tgtcgcatga gccgggtgtt ggctttgcgg
     3001 ctgacgcggc agcgcgcatc aatgccgggc tgggggtagc ggccgttacc tatggggccg
     3061 gggcgctgaa catgatcaac tcggtggcgg cagcctttgc cgaaaagtcg ccgctggtgg
     3121 tgctgtcggg tgggcctggc aagggtgaat cgcgctccgg gctgctgctg caccatcagg
     3181 ccaaaacact cgactcacag ttccatatct tcaaggagat cacctgtgac caggtgcgac
     3241 tcgatgacgc agcccgtgcg ccaaccgaca ttgcccgcgt gctggcgagt tgcctgcggc
     3301 attcagaacc ggtgtacatc gagattccac gcgacatggt ggctgtaact tgcgccgaag
     3361 tggttccaga aacaccgcag ccggttgatc aggatgccct caacgcctgc gttgatgaaa
     3421 tcctggagcg cctgggccgg gccagctcgc cggtactgat ggcgggtgtc gaggtacgcc
     3481 gctatgggct ggaagacaag gtcgcggccc tgtcgcggcg gctcgggctg ccggtggtta
     3541 ccagcttcat gggccgcggc ttgctgtcag gccaggacgc gccactggtc ggcacctaca
     3601 tgggggtagc cggttttcct gaggtcaccc aactggtcga aggctcggac ggcctgtttt
     3661 tgctcggcga gatcatttgc gacaccaact ttgccgtgtc cgagaccaaa atcgacatgc
     3721 gcaaaaccat tcaggcactc aatgggcagg tcaatattgg ctaccacacc tacgacaagc
     3781 tgccattggc cgcgctggtt gaccggatgc tgcagcgcgt cggcagcgtt gacaaagcct
     3841 ttgacatcac gccccaggtc tttccacgcc acctgcaggc cgatgccagc agcatcaacc
     3901 cgaccgacat tgccacggcg gtcaacgacc tgatggccga gcacggcaag atgccaattg
     3961 cctctgacat gggcgactgc ctgtttaccg cgatggaaat agagcacact gcgctggtgg
     4021 cacccggcta ctacgccacc atgggtttcg gcgtgccggc cgggctgggc ctgcaggccg
     4081 ccaccggtga acgcccgctg attctggtgg gtgacggggc gtttcagatg accggctggg
     4141 aattgggcaa ttgcaagcgt tatggctggg atccgatcgt gctgttgttc aacaacgcca
     4201 gttgggaaat gctgcgcact ttccagcccg aatcgtcttt caacgacctg gggcactggg
     4261 gttttgccga aatggccagc ggcctgggcg gcgatggggt acgcgtcggt agccgcgccg
     4321 agctgcaggc ggcgctggcc aaggcaatcg tcaccagggg caaatttcaa ttgattgaaa
     4381 tcaccattcc acgcggtgtg ctgtctgcca cgctggcgcg ctttgtcgcc ggggtgaagc
     4441 ggctcaacgc ggtcaaaaac tgagcagcat gacaacgccc cacgggaaat ggatgaacca
     4501 catgagccac ccacttgaag ccgccgacct ggtcatcaaa ggcatcagac caccgctcaa
     4561 gctcagtgat ttcaaactga tcgcctttga catggattcg accctgatca acatcgaatg
     4621 tgtcgatgaa atcgccgatg cggccggtcg caaggctgaa gtggccgcca tcaccgaggc
     4681 ggccatgcaa ggcgagattg ccgactacaa ggacagtttg cgcaggcgcg tcaggctgct
     4741 caagggcgtt accaccgtgc aaatggattt ggtgctgcat gaacgtctgc agctcaaccc
     4801 cggagccgcc accctggttg cggcctgcca gcgtgccgga ctgaaagtgt tgctggtgtc
     4861 gggcggtttc acctatttca ccgaccatgt ccgcgacctg cttgccattg actacacccg
     4921 ctccaatgtg cttgaagtgg tggacgccaa gctgaccggt cgcttgctcg accagccctg
     4981 gggtgacatc tgtgacggcg acgaaaagcg caagatgttg ctcgcgacct gtgcacagct
     5041 tggcctatcg ccactgcaga gcattgccat gggcgatggt gccaacgaca ttccgatgat
     5101 gagtgcagcc gggctggcag tggcctacca cgccaagccg acggtacgcg actttgccca
     5161 ggtatgcatc aatgcaggcg gactcgatca gttgctgcag gtgtttgcgc cttgaaaacc
     5221 gccccgtcgc cgggactgtc aaggatgtgg tcagtaataa ctttccctat ctggaccgac
     5281 gtttttgggc gcacagcgtc gttgcaagac acttgtttgg ccagcttcac aagcggtttg
     5341 caacaacgca ttgcgcttgt cagtgcggcc gaactggtca aggtatgttt gcttgaaccc
     5401 ttgagacacc cagaagccca agacccacgc gttcactgat gttatgcagt ttgccctgaa
     5461 ataatgaaca gcgacgagtc caggagcctg ccgactgttt ggatcgaaca gcgggcagcg
     5521 gcccagcggc ccagcggccc agcggcccag cggggccagt ttgcgccaga tttcccgacc
     5581 caaagcccag ttgcgagtca agcagtcgac gcaaaacggg ccactgcgat cagtacaccg
     5641 ccaatgcgcc atttgtctga ctggcttctg agttaatccg tcataatctg gcccgctaga
     5701 ggtgccgccc ggtcgggtgg ctgagaaaca ctctttgaac ctgatctggg tcatgccagc
     5761 gtaggaaagc gtcaacaaag cttttttgcc tcgtgtcatc ccgggtattc actaggaacc
     5821 gacatgagcc atccaattct tcctgctgat ctatgggccg acgtgttgtc ggttcgcagc
     5881 gtgcgcccac tggtgcattc gatcaccaat ctggtggtga tgaacttcaa tgccaatgtg
     5941 ctgctggcca tgggcgcatc gccggtgatg gcgcatgccc acgaagaagt tgccgacatg
     6001 gccagcatcg cgcaggcgct ggtgctcaac attggcacgc ttgagccgta ctggatcgag
     6061 agcatgcgca aagcactgga gatcgccagc aaaaagggca taaaaaccgt gctcgaccca
     6121 gtcggagcgg gcgcaacaag ctatagaaac gatagtatca aggtactcct gaagcaggcc
     6181 atgccctcgg tgatccgggg caacgcctcc gagatcatga gcgtggcggg cagcgccgcc
     6241 caaacacgcg gtgtggacag cagcgcggct gtcggtgacg cgctgcaatg cgcccgcgca
     6301 ctggccctgc agaccggtgg cgtggtgtgc gtcagcggcc cggtggacca tgtgttcgat
     6361 gccgacggtc gccacgccag tctgtccaat ggccacgagt ggatgacccg catcaccggt
     6421 gtgggttgct ctgccagtgc cctggtcggt gccttttgtg cggtgcagcc cgatgcctgg
     6481 cgtgccacgc tgtctgccat ggcttacctc ggcgtcgttg gcgaagtggc cgcgcaagcg
     6541 gtgcagcagc gcaaccaggg agtcggcagc ctgcagatag ccctgctgga ccaattgcag
     6601 ttgctcgatc aggccagctt tgagcgtcag ctcagaatgg cggtttcttc atgatcgtgc
     6661 attccgatgt tgctgatgca actgcgcgtg cgaccctgac tcgcgcgcta cgcctgtacc
     6721 tggtgacgga tcaggccagt ttacggggtc gggcgctggc agatgtggtg ctggcggcgc
     6781 tcaagggtgg cgtgacttgc gtgcaactgc gtgagaaaag cctctgcacc cgcgactttg
     6841 ttgccatggg actggcgatg caggacctgc tggcagcttt tgccgtgccc ttgatcatca
     6901 acgaccgcat tgacgtggcg ctggcgtgtg gcgcacaggg tgtgcatctg ggccagtctg
     6961 acatgccggt ggcagtggca cgccgtttgc tgccgccgca agtgttcatt ggcctgtcag
     7021 tggaaaccat ggatgacgtg gcgaatgctg ccatgcagcc ggtcgattac ctgggcgtca
     7081 gcccgattta cccgacaccg accaaaaccg acaccgcgcc gccctgggga ttggacgggc
     7141 tgcggcaggt acgcgctgcc accgccttgc cactggttgc gattggtggc attcagttgg
     7201 cgcaagcggg cgaagtgctg caagccggtg cggatggcct ggcggtggtc agcgccatct
     7261 gctcggctga tgaccctgaa gctgccgcca gacttatttt tgcgcaattg cgcagccaaa
     7321 atgcccggtg attcatcgct caccagttca aacttgattc gtcggctaag cgcgctttca
     7381 tctgtaaatc ctcatgaaca ttgactgttt tcgcttcatt tggattcacc catcgggcgg
     7441 cagcgctgcg ctggcccgtg tcaaaaaggc tcagtcttca agcagcgaca gatcacgcac
     7501 tgcacccttg tcagccgaca tcaccaattt ggcataagcc ctgagggcag cagaaacttt
     7561 gcgcttgcgc ggctgggcgg gtttccagcc cagggcatcc tgctcggcgc ggcgtctggc
     7621 gagttcgtcg tctgacacca acacattgat actgcggccg ggaatgtcga tgcgaatgcg
     7681 atcaccgttg cgcaccagtc cgatggctcc gcccgccgcc gcctcgggcg aagcgtggcc
     7741 aatcgacagc cccgaagtac cacccgaaaa acgcccgtcg gtcaacagcg cacaggcttt
     7801 acccagacct ttggacttga tatacgaggt cgggtagagc atttcctgca tgccggggcc
     7861 acccttgggg ccctcatagc ggacgatgac cacgtcaccg gctttgacct gatcggccag
     7921 gatgtgctcg accgcctcgt cttgtgactc ggtaatgtgt gccgggcctt caaacaccat
     7981 caggctgtca tccacaccag ccgtcttcac cacacagccg tccagcgcaa tattgccgcg
     8041 cagcacggcc aggccgcctt ctttgctgaa ggcatgttcg ccactgcgga tgcagccctc
     8101 ggcgcggtca aggtccaggc tgggccagcg cgcgctttgg ctgaacgcca cttgcgtggg
     8161 aatgccaccg gggccagcca tgtagaaagt cttcaccgcg tccgacggat tgcgcgcaat
     8221 gtcccaccgg tccagcgctt ccttgaaggt cttgctgtgc acggtcggca catcggtgtg
     8281 cagcttgccg gcacggtcca actcgcccag gatgccgtaa atgccaccgg cacggtgcac
     8341 gtcttcgata tggtacttgt tggtgttggg cgccactttg cacaattgcg gcacgctgcg
     8401 cgacagtcgg tcgatatcgg ccagggtgaa gtcaatctcg gcttcctggg caatcgccag
     8461 gatgtgcagg atggtgttgg tggagccgcc catggcaatg tccagggtga tggcgttctc
     8521 aaaggccttg aagcccatgg agcgcggtag cacggtcgtg tcgtcctgct cgtaatagcg
     8581 ctggcacaac tccaccgcca gatgaccggc gcgcttgaac agttgctcgc ggtcggcatg
     8641 cgtggccacc acggtgccgt tgcccggcaa ggccaggccc agcgcctcgg tcaggcagtt
     8701 catggagttg gcggtgaaca tgccggagca ggagccgcag gtcggacagg cggagcgttc
     8761 cacctcggcc acgtcggcat cgctcacctg gtcgtcggcc gccatcacca tggcgtcgat
     8821 caggtcgagt ttcttgaatt cgagcttttg ggtcgccggg ttcagcaaac gggtcttgcc
     8881 ggcttccatc gggccacccg agacaaacac caccgggata ttcagccgca tggccgccat
     8941 cagcatcccc ggcgtgatct tgtcgcagtt ggagatgcac accatggcat cagcacaatg
     9001 ggcattgacc atgtattcga ccgaatcagc aatgatgtcg cggctcggca gcgaatacag
     9061 catgccgtca tggcccatgg caatgccgtc atcaaccgca atggtgttga actctttggc
     9121 gacaccgcct gccgcctcaa tctcgcgcgc caccagttgg ccaaggtctt tcagatgcac
     9181 gtggcccggc acaaactggg taaatgagtt aaccaccgca atgatgggct tgtcgaagtc
     9241 gtcgtctttc atgccggtag cgcgccacag cgagcgagcg ccagccatgt tgcgaccggc
     9301 agtggtggtc ttggaacggt aaacaggcat gatgatgaat cgtcaaaaaa atagaaatca
     9361 aacaggggaa aatgcgcctt ggattatccg ccaacgcctg ccaatgccgc caacatgggt
     9421 actgcgcata cggtttggcg actattgaaa ctggcctacc cgcgcggcct tttccggtag
     9481 ttggcatcca gcgcttgttt ggtgcgctca cggcgttcgt cacggcgctt ttcctcttgc
     9541 gccatcgcct tgcgtttggt atagccggtg ctgtcggccc aggcccacca ggctaccgcc
     9601 agcccaaagg gtgtcatcac aacccaccag gaccagtttg tgacaggggc ccattcgatg
     9661 tatttcaaca ccagcagcgc cagccccaaa ccgagtagat acatgatggc tccttcattt
     9721 aaagttacaa acaacagaca acactttaca agaccgggtc aaccgccgtc cctacaatgg
     9781 cgttaagcaa ctctacccca tttttccaaa ggacaagccc atgaaacgca ggttgattgc
     9841 actgctgact ctcgccacgg tgacaagctc cgtgatggcc gatgccgacc tggcaaagaa
     9901 gaaaaattgc ctggcttgcc acgcggttga caataaggtg gtgggccccg gctac
//
LOCUS       DAOSLL010000092         9946 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6579_length_9946_cov_5.913457, whole genome shotgun sequence.
ACCESSION   DAOSLL010000092 DAOSLL010000000
VERSION     DAOSLL010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9946
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6579_length_9946_cov_5.913457"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PLB25_05780"
     CDS             complement(<1..590)
                     /locus_tag="PLB25_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE41123.1"
                     /translation="MAHIVIMGAGIGGMPAAYEMREILPKEHRVTVVNSVDYFQFVPS
                     NPWVAVGWRTREAITLPIAPYLQRKGIAFIPKAVTQIDAAGSKLTLVGGETLDYDYLV
                     ITTGPKLAFDEIPGAGPVSSGGGGFTHSVCSVDHAQALFADYEEFLKSPGPIVVGAMP
                     GASCFGPAYEFAMILDTDLRKRKLRNKVPITFVTSEP"
     gene            complement(616..3912)
                     /locus_tag="PLB25_05785"
     CDS             complement(616..3912)
                     /locus_tag="PLB25_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOE41124.1"
                     /translation="MQDPTKLGVSGRLAKAFKLNAITPLLALVALLLGFFAVLVTPRE
                     EEPQINVTMANVLIAFPGASSEDVQNMVARPAEQVLSQIAGIEHTYSVSRPGMAVMTV
                     QFKVGVPRTEALVRLYDVLDANQDWLPRDLGTLSPIVKPMGIDDVPVLAVTMWSKEAI
                     AAADLERVAHAMEAELKRVPGTREVKTIGGPSRAVHVWLDPARLRERGVDLLSLKALL
                     ASANVSMPSGSVLDNSGAAGQMLKVETGEFLRSADDVGDLVVGVSKGAPVFLREVARI
                     ETGAQLPQRYVWFTPGAAKVDAASPAGTPVAAPVAGGVYPALTMTVTKKPGTNAVDVA
                     SAVRVRIAELQNTIIPANIETSITRDYGETAAEKANKLIQKLAFATGSVILLVGFALG
                     KREAVIVGAAVILTLTATLFASWAWGFTLNRVSLFALIFSIGILVDDAIVVVENIHRH
                     QQLKPTATLRQIIPLAVDEVGGPTILATLTVIAALLPMAFVSGLMGPYMSPIPINASL
                     GMAISLGIAFTVTPWLALKLMRSQGHAHGHEQPAGKASRLQTLFGRVLTPFLQSSRKR
                     WLLLAGILVALLLSVSLTLLQWVVLKMLPFDNKSEFQVVVEMPAGTPLETTAAALHEL
                     GAYLAQQPEVLNLQAYAGTASPITFNGLVRQYYLRADAEQGDLQVNLVDKHHRDDKSH
                     VIAQRLRPELEKIGQRHHARVKVVEVPPGPPVMSPLVAEVYGPDEAGRQLLASRIAQA
                     FEATPDIVGVDTSLKENAPRAYLRVRRQRAESLGISVAAVAQTAAMALAGADAAYLHD
                     GHAKYPVPVRLQLPLETQVGLDAILALPMRAANGQMVPLSELVQIEQGVIDKPLFTKD
                     LQGLSYVFGDMAGKLDSPLYGLFAIRGKLPDAALPGTGALGEYWISQPTDPYRQYAVK
                     WDGEWQITYETFRDMGAAYGVGLILIYLLVVAQFKSYLTPLVIMAPIPLTIIGVMPGH
                     ALLGSQFTATSMIGMIALAGIIVRNSILLVDFIELQITQGVPFKDAVVQSAAVRAQPI
                     ALTGLAAMIGAFFILDDPIFNGLAISLIFGILVSTVLTLVVIPVLYYALYWRRIKPLE
                     RAAASGDDIH"
     gene            complement(3920..4945)
                     /locus_tag="PLB25_05790"
     CDS             complement(3920..4945)
                     /locus_tag="PLB25_05790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOE41125.1"
                     /translation="MSTLAVAQNTVQVPVVAAQIKAVGNGFELDGVVQPVKQSTVSAQ
                     VSGRLVSLAVKAGDRVSAGQLLATIDDRETQTGVQSSQAQVAQAQAQWRNAQSNFDRT
                     RDLQRQGFVSSAAMDTADAQLKSASAARDQASAGARQAGLSQGFTRVTAPFEGWVLQT
                     QADAGDLAAPGKPLLTLYAPLPLRAVVQVPLSRSSLVSASSLVEVQVAAADGAQQWIR
                     PTQVSHVPGADAVAQTIEWRLELPAQAVKGLLPGQQVRVRFAAGEKQRLVIPAAAVLR
                     RGELTAVYVAGNKGFVLKAVRLGADHGSQGLEVLAGLQAADQVALDPVRAGLSGATAA
                     VTTPAAQ"
     gene            complement(5175..5695)
                     /gene="ssb"
                     /locus_tag="PLB25_05795"
                     /pseudo
     CDS             complement(5175..5695)
                     /gene="ssb"
                     /locus_tag="PLB25_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702067.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            complement(5723..6919)
                     /locus_tag="PLB25_05800"
     CDS             complement(5723..6919)
                     /locus_tag="PLB25_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE41126.1"
                     /translation="MTATELRASFSLSSIFALRMLGLFLILPVFATEAARYPGGDDAA
                     MVGLTMGMYGLTQAMLQFLYGLASDRVGRKRVIVFGLLVFAVGGAVAALAPSLFWLAV
                     GRAIQGAGAVSAAVTALLADQTRDEVRTKSMALVGASIGLMFALSLVAAPVLAAGIGL
                     VGIFALTATLALLGVAAVVWWVPAEPQQHKNLPRGKLRDVLGSVALMRLNLGVFILHA
                     VQLAMWLALPAMLVQAGLPHDDHWQVYLPAVLGSFVLMGGSLFQLEKRGYLRAVFLSS
                     IGLTVLVQLGLWWLTHKTPGLLALGSLLFVFFYAFNVLEASQPSMVSRQAPTNARGAA
                     IGVYNTLQSLGFFAGGLGGGWLVKHGGAQALFAACAGLMLLWLLVAWPMQANQGKATE
                     PISKAG"
     gene            7571..>9946
                     /locus_tag="PLB25_05805"
     CDS             7571..>9946
                     /locus_tag="PLB25_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOE41127.1"
                     /translation="MNTPADGNHLESATDGKYLAAALQQQTISIRGARTHNLKNIDID
                     IPRNQLVVITGLSGSGKSSLAFDTLYAEGQRRYVESLSTYARQFLQLMDKPDVDVIEG
                     LSPAISIEQKATSHNPRSTVGTVTEIHDYLRLLFARAGTPFCPQHQLPLQSQTVSQMV
                     DATLALPVDTRLMILAPLAREKKGEFLDVFADMQAQGYVRFRVNGVAYEFDQLPVLKK
                     TEKHDIDVVIDRIKVRHAPPVATAAAGAVAFDDPLADSADTMQTARADFDALKQRLAE
                     SFEAALRLANGRAIVIEIDSTQRNQDGLYKPKEHLFNAKFACPACGYSIAELEPRLFS
                     FNSPTGACPSCDGLGMQDFFDPARVVAFPTLSLASGAIKGWDRRNAFYFAMLESLAAH
                     YKFDVEQAFESLPQAVQQVVLSGSGEQEIRFYYTMESGNSAGKRVSKSHPFEGIIPNM
                     QRRYRETDSSLVREDLARYRSTQPCADCGGSRLRLAARHVKIGEGAQARAIFELSHHT
                     LAECFDYFSSLRMLGAKGDIAAKVVREISLRLKFLNDVGLTYLSLDRSAETLSGGESQ
                     RIRLASQIGSGLTGVMYVLDEPSIGLHQRDNDRLIDTLKHLRDIGNSVLVVEHDEDMM
                     RAADQIIDLGPGAGVHGGRVMAQGTFEQVKANPASLTGQYLAHILKIDVPGQRTAWLP
                     SEVAKPVADKSKAPRFAPSPAALRRAAREAVHLSTQGEMQALKVIGASGHNLKNVTAA
                     FPVGLFTCVTGVSGSGKSTLVNDTLYKAVARTIYRAHDEPAEHSAIEGIEYF"
BASE COUNT         2075 a         3187 c         2873 g         1811 t
ORIGIN      
        1 ggctcgctgg tgacgaaggt gatcggcacc ttgttgcgca gcttgcgctt gcgcaggtcg
       61 gtatcaagaa tcatggcaaa ttcataggcc gggccaaaac agctcgctcc cggcatggca
      121 ccgaccacaa tcgggccggg acttttcaga aattcttcat agtcggcaaa aagcgcctgt
      181 gcgtggtcca cgctgcaaac cgaatgggtg aagccgccac cacccgagct gaccggacct
      241 gcgccgggaa tctcatcgaa cgccagtttg ggcccagtgg tgatcaccag gtagtcatag
      301 tccagcgttt cgccgccgac cagcgtgagc ttgctgcctg ccgcatcgat ttgcgtgacg
      361 gccttgggaa tgaaagcgat acccttacgc tgcaaatagg gggcaatcgg cagcgtgatc
      421 gcctcacgcg tgcgccaccc tacagccacc caggggttgc tgggcacaaa ctggaagtag
      481 tcaacgctgt tgaccaccgt gacgcggtgt tccttgggca gaatttcgcg catttcgtaa
      541 gctgccggca tgccgccaat gccagcaccc atgatgacga tgtgagccat gtcttgtctc
      601 ctgctgtaaa agtagttaat gaatgtcgtc gccagaagcc gccgcccttt caagcggctt
      661 gatacgccgc cagtacagcg cgtaatacaa gaccggtatg acgaccagcg tcagcaccgt
      721 cgaaaccaga atgccaaaaa tcagggaaat cgccagtccg ttgaaaatcg ggtcgtccag
      781 gatgaagaaa gcgccgatca tggccgccag accggtcaac gcaatcggct gtgcacgcac
      841 tgctgccgac tgcaccacgg catccttgaa cggcacaccc tgcgtgattt gcaattcgat
      901 gaaatccacc agcaagatgg aattgcgcac aatgatgccc gccagcgcaa tcatgccgat
      961 catcgaagtg gcggtaaatt gcgatcccag caaggcatgt cctggcatga caccaatgat
     1021 ggtcaaggga atcggtgcca tgatcaccag cggcgtcagg taacttttga actgcgccac
     1081 caccagcagg taaatcagga tcaggccgac accgtaggcg gcgcccatgt cacggaaggt
     1141 ttcataggta atttgccatt cgccgtccca cttgacggcg tattggcggt aggggtcggt
     1201 tggttggctg atccagtact cgcccaatgc gccggtgccg ggcagcgccg cgtccggcaa
     1261 cttgccccta atggcaaata gcccatacag cggcgagtcg agcttgcctg ccatgtcacc
     1321 aaaaacataa ctgagccctt gcaagtcttt ggtgaacagc ggcttgtcga tcaccccttg
     1381 ctcaatctgc accagttcgg acagcggcac catttgcccg ttggcggccc gcattggcag
     1441 ggccagaatt gcgtccagcc caacctgtgt ctccagtggc aattgcagac gcaccggcac
     1501 cgggtacttg gcgtggccat cgtgcagata cgcggcatcg gcgccggcca gggccatggc
     1561 agcggtttgc gccacggcag ccactgaaat gcccagcgac tcggcgcgtt gccgacgcac
     1621 cctgaggtag gcgcgcggcg cattttcttt cagcgaagta tcgacgccaa caatgtccgg
     1681 ggtcgcctca aaggcttgcg caatccggct ggcaagcagt tggcggcccg cttcatcggg
     1741 gccatagacc tcggctacca ggggtgacat gactggcgga ccgggcggca cctccaccac
     1801 cttcacccgt gcatgatggc gttgcccaat tttttccagc tccggtcgca agcgctgggc
     1861 aatcacatgg cttttgtcgt cgcggtgatg tttgtccacc aggttgacct gcaagtcacc
     1921 ctgctcggca tcggcgcgca ggtaatactg ccgcaccaga ccgttgaagg tgatcggcga
     1981 cgcagtgccc gcgtaagctt gcaggttcaa gacctcaggc tgctgcgcca aataggcacc
     2041 gagttcgtgc aaggcggcgg cggtggtttc caggggtgtg cctgcgggca tttccaccac
     2101 cacctgaaac tcgcttttat tgtcgaaggg caacattttc agcacgaccc attgcaacag
     2161 ggtcaagctg accgacaaca gcaaagccac aagaatgcct gccagcaaca gccagcgctt
     2221 gcgcgagctc tgcaaaaacg gtgtcaacac ccggccaaac agggtctgca atctggaagc
     2281 cttgccagcg ggctgttcgt ggccatgggc gtggccttgc gagcgcatca gcttgagcgc
     2341 cagccagggt gtcacggtaa aggcaattcc cagagaaatc gccatgccca gactggcatt
     2401 gatagggatc gggctcatgt aaggccccat caagccgctg acaaaggcca tcggcaatag
     2461 cgcggcaatc accgtcagcg tcgccagaat ggtcgggcca ccgacttcat cgacggccag
     2521 cggaatgatc tggcgcagcg tggctgtggg tttgagttgc tggtgccggt ggatgttttc
     2581 caccaccaca atcgcatcat cgaccaggat gccaatcgaa aaaatcagcg caaacagcga
     2641 cacccggttc agggtaaaac cccaggccca ggacgcaaac aaggtagctg tcaaggtcag
     2701 aatcaccgct gccccgacga tcactgcctc acgtttgccc agcgcaaagc cgaccagcaa
     2761 gatcaccgag ccggtcgcaa acgccagttt ctgaatcagc ttgttggctt tttcggccgc
     2821 tgtttcacca tagtcacggg taatgctggt ctcgatgttg gcgggaatga tggtgttttg
     2881 cagttccgca atacgcacac gtaccgcact ggccacatca accgcgttgg tccccggctt
     2941 tttggttacc gtcatggtca gtgccggata gacaccgcca gcgactggcg cagcaactgg
     3001 tgtgccagcg ggcgatgcag cgtccacctt ggccgcgcca ggggtaaacc agacatagcg
     3061 ttgcggcaat tgggcaccgg tctcgatgcg cgccacttcg cgcagaaaca ccggcgcacc
     3121 cttgctgacc cccaccacca ggtcgccaac gtcatcagcc gagcgcaaaa actcgccggt
     3181 ttcgaccttc agcatctgcc cggctgctcc cgaattgtcc agtaccgatc cggaaggcat
     3241 gctgacattg gccgaggcca gcaaggcctt gagcgacagc agatcaaccc cccgctcgcg
     3301 cagacgcgcc gggtccagcc agacatgcac tgcgcgcgac gggccgccaa tggttttcac
     3361 ttcacgggta cctggcaccc gcttgagttc ggcctccatg gcatgcgcga cacgctccag
     3421 atcggccgcc gcaatcgcct ctttgctcca catcgtcacc gccagcaccg gtacgtcatc
     3481 gatacccatg ggcttgacga tcgggcttag cgtgcccaga tcacgcggca gccagtcctg
     3541 attggcatcc agaacatcat acaaacgcac cagcgcctcg gttctgggaa cacccacctt
     3601 gaactgcacc gtcatcaccg ccatgccagg gcgcgacacc gaataagtgt gctcgatacc
     3661 ggcgatctgg ctgagcactt gctcggccgg tcgtgccacc atgttttgca cgtcctcgct
     3721 gctggcccct ggaaacgcaa tcagcacgtt ggccatggtc acattgatct ggggctcttc
     3781 ttcgcgcggt gttaccagca ccgcaaaaaa acccagcagc aaggccacca acgccagcag
     3841 cggggtgatg gcattgagct tgaaggcctt ggccagtcgg ccggaaaccc ctagtttggt
     3901 cggatcttgc atgctgggct cactgtgctg cgggtgtggt cacagccgct gtggcaccac
     3961 tcagcccggc tctgaccggg tccaacgcca cctgatctgc cgcctgcaga cccgccagca
     4021 cctcaaggcc ctgactgcca tgatcagcgc caaggcgaac cgccttcagc acaaaaccct
     4081 tattgcccgc tacatagaca gcggtcagtt cgccacgccg cagcacggcc gcggccggta
     4141 tcaccaggcg ctgtttctca ccggcggcaa aacgcacccg cacctgctgg ccaggcaaca
     4201 ggcctttgac cgcctgcgcc ggcaattcca gccgccactc aatggtctgt gctaccgcgt
     4261 cagcgccggg cacatggctg acttgtgtcg ggcgaatcca ctgttgcgcg ccatctgccg
     4321 cagccacctg aacttctacc aggctgctgg cgctgaccag gctggacctt gatagcggca
     4381 cctgtaccac cgcgcgcaac ggtagcgggg cgtaaagcgt cagcaacggc ttgcccggcg
     4441 cggccaggtc accggcatca gcctgggttt gcagcaccca gccctcaaac ggtgccgtca
     4501 cgcgggtaaa cccctgcgac aaaccggcct gcctggcccc ggcactggcc tgatcgcgcg
     4561 ccgccgaggc agacttgagc tgggcatctg cggtgtccat ggcggctgaa ctgacaaagc
     4621 cctggcgctg caaatcacgg gtgcggtcaa agttggactg cgcattgcgc cactgcgcct
     4681 gtgcctgggc tacctgggcc tgactgcttt gcacgccggt ctgcgtctcg cggtcgtcaa
     4741 tggtcgccag caattgcccg gccgacaccc ggtcaccggc tttgacggcc agggaaacca
     4801 ggcgacccga gacctgtgcc gacaccgtgc tttgcttgac cggctgcacc acgccatcaa
     4861 gctcgaaacc attgccaacc gccttgatct gtgccgcgac cactggcacc tgcacggtgt
     4921 tttgcgcaac ggccagggtt gacatcagca gcaccgccag gcccggcaac aagcgcctgg
     4981 gttgaacctg gtgccacaca ttgagcaaaa aaaagtcagg catggaattt ccttcaaaat
     5041 acggtaggga gtatattgga aactggcgcg aatggtactg gcggctgggg acaagcaatg
     5101 aaaaaaccca caagaccagc gaggtcttgt gggtttttgc gatgttttgg cagcagttct
     5161 gccagttggc caagtcagaa cgggatatcg tcgtccatat cgtcaaagcc gctggccgga
     5221 cgggtggcgg cgctggagcc ggtgcgggta ccggggctgg ccgggccgcc atcgggcgag
     5281 gcgccggtgc cgggcgggaa taactattgc ctgcaccact gccaaaaccg tcgtcatcgc
     5341 cagatggcgc acccatgccc tggcggctac cgagcatctg catctgcagg gcctggatgt
     5401 cagtggtgta gcgctcgatg ccatccttgt cggtgtattt gcgggtgcgc aggctacctt
     5461 caacataaac ttgcgagccc ttgcgcaggt attgctcggc gatttcggcc agtcgaccag
     5521 tgaactggac gcgatgccac tcggtcgcct cacgcagctc attggtctgc ttgtctttcc
     5581 atttgtcggt ggtggccaac gcgacattgg ccacacggtc accgctggga aaagtccgca
     5641 cctccggatc gcgccccaaa ttccccacca aaatcacttt gttcaccgat gccatgttgc
     5701 ttccctcgtc aaaaggttca gtttatccgg ctttgctgat cggctcggtg gcttttcctt
     5761 ggttggcctg catcggccag gccaccagca gccacaacag catcagcccg gcacaggccg
     5821 cgaacaaggc ctgggcaccg ccatgtttga ccagccagcc gccgcccagg ccaccggcaa
     5881 aaaatcccag cgactgcagc gtgttgtaaa ccccgatggc agcgccgcgc gcattggtgg
     5941 gggcctggcg cgacaccatg ctgggctggc tggcctcaag cacattgaac gcgtaaaaaa
     6001 agacaaacag caagctgccc agcgccagca ggccgggggt tttgtgcgtc aaccaccaca
     6061 accccagttg caccagcacg gtcaacccga tcgagcttaa aaacaccgcc cgcaggtagc
     6121 cccgcttttc gagctgaaac aaactgccgc ccatcagcac aaacgagccc agcacggccg
     6181 gcaaatagac ctgccaatgg tcgtcatgag gcaaaccggc ttgcaccagc atggcgggca
     6241 gcgccagcca catcgccagt tgcacggcat gcaaaataaa aacgcccagg ttcaggcgca
     6301 tcagcgcgac cgacccgagc acatcgcgca acttgccgcg tggcaggttc ttatgctgtt
     6361 gcggctcggc cggcacccac caaaccacgg ctgctacgcc gagcagcgcc agggtggcag
     6421 tcagcgcaaa aatccccacc agcccgatgc cagcggccag caccggtgcc gccaccagcg
     6481 acagcgcaaa catcaagccg atgctggcac caaccagcgc catcgacttg gtgcgcacct
     6541 cgtcacgggt ctggtcagcc agcaaggcgg tcaccgcagc cgacaccgcg cccgcgccct
     6601 gaatggcacg ccccaccgcc agccaaaaca gcgacggcgc cagtgccgcc accgcccccc
     6661 cgaccgcgaa caccaacagc ccgaacacga tcacccgttt gcgcccgacc cggtccgaag
     6721 ccagtccata caagaactgc agcatggctt gcgtcaaccc gtacatgccc atggtcaggc
     6781 cgaccatggc cgcatcgtcg ccgccaggat agcgggcagc ttcggtggca aacaccggca
     6841 agatcaagaa cagccccagc atgcgcaggg cgaagatgga tgacagcgaa aagctggcac
     6901 gcagttcggt cgctgtcatc ctggtggaca gtgaaacact ggcagattcc agcggggcat
     6961 tcgcttgagg caaaaaatat ctccgggttc acacaatcag gccgcgttgg acggcagatg
     7021 ccggattgtc ccttatcacc tgcagcagta ccgaaccgac ccgctgcaag ctgctgatgc
     7081 catttccaaa ccatccaggt cattgtcaac ttgccttgcc ttgccttgct tgcgcagtat
     7141 ctgcgccaga cttgcaggca cgaatgacta aggatgtggt cagaaataac ttcccctatt
     7201 tgcaccggcg gatttgggcg cacagcgtcg ttgcaagacg cttgtttagc cagctaaacg
     7261 gcgcgtcttg cgcctagccg cgcatcccaa ctccttggtg caaaacgggg agttattccc
     7321 gaccacatcc taaaaagggg tgagcgctca cgcacttgcg ttcaaaccgc tgtcgatggt
     7381 cgtccggcac tgaaagtctg accacgccaa caatgccacc gcagccgcct tgacagcaac
     7441 caggacagcg gtgatcgccg cactgcgcgc agcgcaggct gcgccagctt tgtcggcgat
     7501 gatcaggcat tgactatcat gctgggtttc ccggtgcatt ttcaccgtca cctaattgag
     7561 tcccatcgcc ttgaacacgc ctgcagacgg caaccacctc gaatccgcaa ccgatggcaa
     7621 atacctagct gccgcgctgc agcagcagac catcagcatt cgcggtgcgc gcacgcacaa
     7681 cctgaaaaat atcgacatcg acatcccgcg caatcagttg gtggtgatca ctggcttgag
     7741 tggctcgggc aagtccagtc tggcctttga caccctgtac gccgagggcc agcgccgcta
     7801 tgtcgagagc ctgtccactt acgcgcgcca gtttttgcaa ctgatggaca agcctgatgt
     7861 cgatgtgatt gaaggcttgt cgccggccat ctcgatcgag caaaaggcca ccagccacaa
     7921 cccgcgctcg accgtcggca cggtgaccga gattcacgac tacctgcgcc tgctatttgc
     7981 ccgtgctggc acgccgtttt gcccgcagca ccagttgcca ctgcaatcac aaaccgtcag
     8041 tcaaatggtc gatgccacgc tggccttgcc ggtggacacc cggctgatga ttctggcacc
     8101 gctggcgcgg gaaaaaaaag gcgagtttct ggacgtgttt gccgacatgc aggcacaagg
     8161 ctatgtgcgc tttcgcgtca acggcgtggc ctatgagttt gaccagttgc cggtattgaa
     8221 gaaaaccgag aagcacgata ttgatgtggt gattgaccgc atcaaggtgc gtcacgcgcc
     8281 gccggttgct acggcggcgg caggcgctgt cgcgttcgat gaccccctgg ccgactctgc
     8341 tgacaccatg cagacagcgc gggctgattt tgatgccctc aaacagcgcc tggccgagag
     8401 ttttgaggcg gctctacggc ttgccaacgg gcgcgccatt gtcatcgaaa tagatagcac
     8461 gcagcgcaat caagacgggc tatacaagcc aaaagagcac ttatttaacg ccaagttcgc
     8521 gtgcccggcg tgcggctact cgattgccga actggagccg cgcctgttct cgttcaactc
     8581 gccaacaggg gcctgcccca gttgcgacgg tctgggcatg caagattttt ttgacccggc
     8641 acgggtggtg gctttcccga ccctgagtct ggccagcggg gccatcaagg gctgggaccg
     8701 gcgcaacgcc ttttactttg ccatgctcga gagcctggct gcgcactaca agtttgacgt
     8761 cgagcaggcc ttcgagtcac tgccgcaagc ggtgcaacaa gtcgtgttgt cgggctcggg
     8821 tgagcaagag atcaggttct actacaccat ggagtccggt aacagcgcgg gcaagcgggt
     8881 cagcaaaagt cacccgttcg agggcatcat ccccaacatg cagcggcgtt accgcgagac
     8941 cgactccagc ctggtgcgcg aagacctggc gcgttaccgg tcaacccagc cctgtgcaga
     9001 ctgcggcggc agccgccttc ggctggcggc acgccacgtc aagattggcg aaggtgccca
     9061 ggcgcgcgcc atttttgaac tcagccatca caccctggcc gagtgttttg attacttcag
     9121 cagcctgcgc atgctcggtg ccaagggcga cattgccgcc aaggtggtgc gtgaaatcag
     9181 cttgcgcctg aagtttctca acgatgtggg cctgacttac ctcagcctgg accgcagtgc
     9241 cgagaccttg agcggtggcg aatcgcagcg catccgtctg gccagccaga ttggctcggg
     9301 gctcacaggt gtcatgtatg tgctcgacga gcccagcatt ggcctgcacc agcgcgacaa
     9361 cgaccggctc atcgacaccc tgaaacacct gcgcgacatc ggcaacagcg tgctggtggt
     9421 cgagcatgac gaagacatga tgcgtgcggc tgatcagatc atcgacctgg ggccaggtgc
     9481 tggcgtgcac ggcgggcggg tcatggcaca gggcacattt gagcaggtca aggccaaccc
     9541 ggcatcgctg actggccagt atctggcgca catcctgaaa atcgatgtgc cgggccagcg
     9601 caccgcctgg ctgcccagtg aagtcgccaa accggttgca gacaagtcca aggcgccgcg
     9661 ctttgccccc agcccggctg cgctgcgccg cgccgcacgc gaagccgttc acctgtccac
     9721 gcagggcgag atgcaggcgc tcaaggtcat tggggccagc ggccacaacc tcaaaaatgt
     9781 gactgctgcc tttccggtcg ggctgttcac ctgtgtcacc ggtgtctcgg ggtcaggtaa
     9841 atcgaccttg gtcaacgata cgctgtacaa ggcggtggcg cgcaccattt accgggcgca
     9901 tgacgaaccg gcggagcact cggcgattga aggcattgag tacttt
//
LOCUS       DAOSLL010000093         9883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6627_length_9883_cov_4.994302, whole genome shotgun sequence.
ACCESSION   DAOSLL010000093 DAOSLL010000000
VERSION     DAOSLL010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9883
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6627_length_9883_cov_4.994302"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(353..925)
                     /locus_tag="PLB25_05810"
     CDS             complement(353..925)
                     /locus_tag="PLB25_05810"
                     /inference="COORDINATES: protein motif:HMM:NF014825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HOE41128.1"
                     /translation="MTPAKPDPYQPCPCGSGRKYKFCCRIKEQQLSNESPLVLVQKSA
                     QYPLSQCAITRHWQPSGMGSVFVVRQLPNSKFLFGVYLVDLLCLGVKNTFFNVNVGPD
                     AIHSMLTLQEMPIDPIDYEDARSIILGSIEFARKHGFEPHPDWNHTRHLVEPDRPFKH
                     KFAFGQKGKPIYIQGPDDDFVAITSMLKRK"
     gene            complement(1029..2825)
                     /locus_tag="PLB25_05815"
     CDS             complement(1029..2825)
                     /locus_tag="PLB25_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE41129.1"
                     /translation="MIRLTELKLPLSALPLVTRRAADAPAESGADRLPDLHPLAALRQ
                     LCATALGLAPSDITQLQVFKRSFDARKAALLAVYCVDLALADAAQEASVLARFQNHPH
                     IGPTPDMAYRLVARAPANLPLRPVVVGFGPCGIFAALLLAQMGFRPIVLERGKPVRER
                     TRDTWDLWRKHQLNPESNVQFGEGGAGTFSDGKLWSQIKDPRHLGRKVMEEFVKAGAP
                     AEILVEAHPHIGTFKLVKVVENLREQITALGGEIRFGQLVTDIEVDGAPARRQVRGLS
                     VRNQTTGQINEIRADQVIMALGHSARDTFAMLHQRGVMIEAKPFSVGFRIEHPQSLID
                     RARWGRHAGHPSLGAADYKLVHHASNGRGVYSFCMCPGGMVVAATSEPGRVVTNGMSQ
                     YSRNERNANAGLVVGIEPADFPQDSTAFIEAFGAVAGSEYAQQAIESRAFEARSVHPL
                     AGINLQRQLESGAFLLGGSNYEAPGQLVGEFLRGQASGSLGSVQPSYQPGVRLCDLSL
                     SLPDYAIAALREALPVFGKKIKGFDMPDAVLTGVETRTSSPLRLPRGDDYQSINTAGL
                     YPGGEGAGYAGGILSAGVDGIKLAEALARSLT"
     gene            3593..4651
                     /locus_tag="PLB25_05820"
     CDS             3593..4651
                     /locus_tag="PLB25_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HOE41130.1"
                     /translation="MLTFYSDRHHLHHGSSELFQGQVLPCFEMPRRADNVLARVRELG
                     LGEVRPPQAFGLEPVLRVHDPGFVLFLSTAWDEWALTGRTDDALPLVWPVRGARLGRI
                     PQDIGGKFGYYAMDPGVPITAGSWTAIQDSANVALSGARALLAGVRAVFSLCRPPGHH
                     AAARSMGGYCYLNNAAIAAQYMRDQGCPRVAILDVDYHHGNGTQSIFYDRADVLYVSI
                     HADPKVEYPAFLGYADETGSGPGLGSNHNYPLPHGTAWTTYALVLIDACQKIQMYGPD
                     ALVVSLGVDTFEKEPMSAFLLKNDDYLRMGDSIGRLGLPTLFVMEGGYALDDIGTNVV
                     NVLTGFEAANPFRVLQKA"
     gene            complement(4928..5194)
                     /locus_tag="PLB25_05825"
     CDS             complement(4928..5194)
                     /locus_tag="PLB25_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41131.1"
                     /translation="MSTEKVTLGSGPGFGRTMIEPKLLPLYGNLRLSDLLLLISATLP
                     GWPPMLLIPERYVQSAAALPAASHTVANMTIALVDWWLIGAIDS"
     gene            complement(5695..7773)
                     /locus_tag="PLB25_05830"
     CDS             complement(5695..7773)
                     /locus_tag="PLB25_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HOE41132.1"
                     /translation="MNHLNAHFDADTRQIIGACPHDCPDTCSMLTTVANGVAIKVQGN
                     PAHPHTDGALCTKVSRYPERTYHRDRILQPLKRIGPKGAGQFEAVSWDAALFDIAQRL
                     SAIARRDPQAILPYSYGGTMGLVQGDSMSARFFHKLGASLLNRTICATAGGEALLHTL
                     GIKTGMRVEFFAESKLILIWGSNSISSNLHFWRYANQAKRNGAKLICIDPRKSETADK
                     CHEHIALLPGTDGALALSIMQQLITHDWLDHDYLARYTLGWPALQQRALQWPPERAAT
                     VCGIDAGQIRSLARDYGASVKSGEPAAIRMNYGLQRVHGGGNAVRLIAMLPALTGAWR
                     QRAGGMLLSSSGNFPVQRQLLERPDLLAGRSPRSINMATIGDDLLRTASAEFGPAIEA
                     LIVYNSNPVAIAPDSARVVQVFARDNLFTVVLEHFQTDTADFADYILPATTQLEHWDV
                     HLSYGHTDVLLNRPAIAPLGQAKPNTQIFRELSQRMGFDDACFADSDEALCRMAYGEA
                     LDFNTLLTQGFATLATPEAPFAQGNFPTPSGKCEFFSQRLADRGQDGLPDHVPNYEAA
                     NTSATYPLAMISPPARNFLNSSFVNVNSLQAMESEPLLEMHASDASARGIAQGDMVRV
                     FNERGEYRCRVNVGTRARPGVVNGLGVWWRKLGPSGTNVNQLTSQRLTDLGAAPTFYD
                     CLVQVQPVAA"
     gene            complement(7948..9411)
                     /locus_tag="PLB25_05835"
     CDS             complement(7948..9411)
                     /locus_tag="PLB25_05835"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOE41133.1"
                     /translation="MTAYDQFLASHPDYAATGALDDLRAAQYGRLDAQDEVYLDYTGA
                     GLHAASQVHEHAQMLSDHVLGNPHSASPSSMATTHRVEQARAAVLAYFNGTGDYTAIF
                     TLNASGALKLVGESYPFAPCGKLLLTADNHNSVNGMREFARAKGLLASYAPLTRPELR
                     IDSGAMDALLAQADPTQANLLAFPAQSNFSGVKHPLELVARAQAQGWHVLLDAAAFVP
                     TNRLDLRAVRPDFVVMSFYKMFGYPTGVGCLLVRNATLKALRRPWFGGGTVNFATVQA
                     RLHVLSDGEAGFEDGTLNYLGIPAVEIGLRHLQQVGIETIQTRVNCLTGWLITQLLAL
                     RHSNGQPMVRLYGPANMTMRGATVTMNVYDPHGHLVDYRRVEELAGQAHISLRTGCFC
                     NPGAGETAEDLGEEEMRLGIAQAGDEINLLRFSQALQARSGKSAGAIRVSTGIASNFA
                     DVARFLAFVAGLKDQTSLAIGSVSFDIESCRVVRDGG"
BASE COUNT         1976 a         2881 c         3015 g         2011 t
ORIGIN      
        1 cgcccaaatc cgccggtgca aataggggaa gttatttctg accacatcct tagtctccaa
       61 caagtcaagt ctttgcaaat catgtgaaga attcagccat tcaaagtcat tcgtcgcgag
      121 ggcgcgcctc ctacaaattc aaataggccc cgactgtttg tttaggagtc gcgccctcgc
      181 gacgaaggct catcgattcc ggctcgtccg gcttgggatg tggtcgggaa taactccccg
      241 ttttgcacca aggagttgat tttgtcgcaa cgaaaagtag cctttggcgg gccgggcttg
      301 cttgacagtc tggtgctggt gcccggttgc acaggcagca cagcgcggcc catcatttgc
      361 gtttgagcat tgaggtgatg gcgacgaagt catcgtcggg accctggata tagatgggct
      421 tgcctttttg gccaaatgca aacttgtgct tgaagggccg gtccggttca accaggtgcc
      481 gggtatggtt ccagtcggga tgtggctcaa aaccatgctt gcgggcaaat tcgatgctgc
      541 cgaggatgat gctgcgggca tcttcataat cgatcggatc gatcggcatt tcttgcagtg
      601 tcaacatcga atgaatggca tcagggccga cgttgacgtt gaaaaaggtg ttcttgacac
      661 ccaggcacag caggtcaacc agatacaccc cgaacaggaa ttttgaattt ggcaactggc
      721 gaaccacaaa aacactgccc atcccgcttg gctgccagtg cctggtgatg gcgcattggc
      781 ttagcgggta ttgggctgac ttctggacca gcacaagcgg ggattcgttg ctgagctgct
      841 gctctttgat gcggcagcag aacttgtatt tgcgcccgct gccgcagggg cagggctggt
      901 aggggtcggg tttggcaggt gtcatgagga tgcggtcaat ggaggagtga aatgtgaacg
      961 cgggtgccga tgcagcccca attttgcctg cctttgccgg gcttttcgtg cctcaggtca
     1021 ggggtcgatc aggtcagtga gcgtgccagt gcctcggcca gcttgatgcc atccacccca
     1081 gccgacaaaa tgccgccagc gtagccagca ccttcgccgc cggggtacag ccctgccgtg
     1141 ttgatgctct gatagtcgtc gccacgcggc aaacgcaacg gcgatgaggt gcgggtttcc
     1201 acgccggtca gcaccgcgtc gggcatgtca aagcctttga tctttttacc aaagaccggt
     1261 agcgcctcgc gcagcgcggc aatcgcgtaa tccggcaggc tcaaactcag gtcgcacagc
     1321 ctgacccccg gctggtacga cggttgaacc gagcccaggc tgcccgatgc ctggccgcgc
     1381 aagaattcac cgaccaattg ccccggtgcc tcatagttgc tgccacccag caaaaacgct
     1441 ccggattcca gttgtcgctg caggttgatg cccgccagcg gatgcaccga gcgggcttca
     1501 aatgctcttg attcaatagc ttgctgcgca tattcagagc ccgctacagc cccaaaagcc
     1561 tctatgaaag cagtcgaatc ctggggaaag tcagcaggct cgataccgac caccaggcca
     1621 gcattggcat tgcgctcgtt gcgcgaatac tggctcatgc cgttggtcac gacccggccc
     1681 ggctcgctgg tggcggccac caccatgccg cccgggcaca tgcaaaagct gtaaacgccg
     1741 cgaccattgc tggcgtggtg caccagtttg tagtccgccg cccccaggct ggggtggcca
     1801 gcatggcggc cccagcgggc gcgatcgatc aggctttgcg ggtgctcaat gcgaaagccg
     1861 accgaaaacg gtttggcctc gatcatcaca ccgcgctggt gcagcatggc aaaggtgtcg
     1921 cgcgcgctgt ggcccagcgc catgatgacc tggtcagcgc ggatttcatt gatctggccg
     1981 gtggtctggt tgcgcacgct caggccgcgc acctgtcgtc tggcgggcgc gccatccacc
     2041 tcaatgtcgg tcaccagttg cccgaaacgg atttcgccgc ccagcgcggt gatctgctcc
     2101 cgcaggtttt ccaccacttt gaccagtttg aaggtgccga tgtgcgggtg cgcttcaacc
     2161 aggatttcgg ctggcgcacc ggccttgaca aattcttcca tcaccttgcg gcccagatgc
     2221 cgggggtcct tgatctggct ccacagcttg ccatcagaaa aggtgccagc accgccttcg
     2281 ccaaactgca cattgctttc ggggttgagc tgatgtttgc gccacaaatc ccaggtgtcc
     2341 cgggtgcgtt cgcgcaccgg tttgccgcgc tcaagcacga ttggccgaaa acccatttgc
     2401 gccaacaaca gtgcggcaaa aatgccgcaa ggcccaaacc ccaccaccac cgggcgcagc
     2461 ggcaaattgg ccggagcccg tgccaccagc cggtaagcca tgtcaggtgt cggcccgatg
     2521 tgcgggtggt tttgaaaacg cgccagcacc gaggcctctt gcgcggcatc ggccaacgca
     2581 aggtccacgc aatagaccgc cagcagagcc gccttgcgcg catcaaaact gcgtttgaag
     2641 acctgcaatt gcgtgatgtc ggaaggtgca agacccagcg cagtggcaca aagttgtctc
     2701 agggctgcca gcggatgcaa gtcaggcagc cggtcggcgc cagactcggc gggcgcatcg
     2761 gcagcgcggc gagtcacaag cggcagggca gacaggggca gtttgagttc agtcagtcgg
     2821 atcatggcgg gctcgtgttc atcaagcagg ccccgatgtt aaagcgccgc ccagcatcgg
     2881 cgtgatcagc aaggccaggg catatttgtc tctatttggc agtcccgcct gcgccggaaa
     2941 tgaaggtagg ttaatggctt ggcgcaatcg caaaattgcc ctggtcacaa cggcaaccgg
     3001 cgcttgaacc tggattcctc ggttgaccgc ttgctttcat ttgcaagtcc ttatgagcat
     3061 ttacttcttt agcttcgatg aggttcaccc attggttgac agcgcgacgc acccgattga
     3121 accgctggca acacgcctgg gggtgttact ccggctggcg ggcaggagct gtgctgcgtc
     3181 gtcgcccctg cgggacgcag ccatgccaca aggctggcct gcttcaaacc gtccaaacgt
     3241 gcgcagccac ggttggcacc aaggcttgtg ccttacccag acgcgccgcc agcggcccac
     3301 tcgggcgcgt ccaacctagc aggatgaatt tgttcttatg gcatcttcgt cattcccgcc
     3361 tgcgcggcaa tgacggtaaa tcaacgactt ggccaaacaa caaatttgcc ctgtccaact
     3421 gaggaaacta gcttgaatga aaagtgattt gctttgacaa tggcgggcta cacgcaaccg
     3481 gctttggtga ccctcatggt ttcaatttcg tccattgcct gcttgaacat ggcacattgc
     3541 catgcccggc ccgccgcgtg tgcccgccag acagtcagca gaccgttttg tcatgcttac
     3601 tttctacagt gatcgccacc atttgcacca tggcagcagc gagctgttcc aggggcaggt
     3661 gctgccttgt tttgagatgc cgcgccgtgc tgacaacgtg ctggcgcggg tgcgtgagct
     3721 tgggttgggc gaggtacggc caccgcaagc ctttggtctg gagccggtgc tgcgggtgca
     3781 tgaccccggt tttgtgcttt ttttgtccac cgcctgggat gagtgggcgc tgactggccg
     3841 caccgacgat gccttgccgc tggtgtggcc ggtgcgaggt gcgcgcctgg gtcgcattcc
     3901 acaagacatt ggcggcaagt ttggctacta cgcgatggac cctggcgtgc cgattaccgc
     3961 cggaagctgg accgcgattc aggactctgc caatgtggcc ctgagtggtg cacgcgccct
     4021 gctggccggt gtgcgcgccg ttttttcact gtgccgcccg ccgggtcacc atgcagcggc
     4081 cagatccatg ggcggctact gttacctcaa taatgctgcc atcgcagcgc agtacatgcg
     4141 tgaccagggt tgcccgcggg tggccatact cgatgttgat taccatcatg gcaacggtac
     4201 ccaaagcatt ttttatgacc gtgccgacgt gctgtatgtc tcgattcatg ccgatcccaa
     4261 ggttgagtac ccggcttttc tgggctacgc cgatgaaacc ggaagcgggc ccggcttggg
     4321 ctccaatcac aactacccgc tgccacatgg caccgcctgg accacctacg cactggtgct
     4381 catcgatgcc tgccagaaaa tacagatgta cggacctgat gcactggtgg tgtcgctggg
     4441 agtcgatacc tttgaaaagg agccgatgtc ggcctttttg ttaaaaaacg acgactacct
     4501 gcgcatgggg gacagcatcg gccgactggg gctgccgacc ctgtttgtca tggagggtgg
     4561 ttacgcgctt gacgacattg gcaccaatgt ggtcaatgtg ttgaccggat ttgaggccgc
     4621 caatccgttc agggtgttac aaaaagcata gcgtgtagcg ctcttgcaga aagggctgcg
     4681 ggcatatttt ctataaaagc gtgtgggccg cttcaggtcg gcgaggcaac tgggctgggc
     4741 tgatggccat gtggcgggcg atactcggcc cattctgatc gcaaggccaa tccgcaaagc
     4801 agcaagattc attaccctgg catgcaaaca catcattggg caagagttga aagctgcaaa
     4861 agaaacatga aacatcaccc tattcagaaa cgctgtagcg ctttgagttt gatcgccgca
     4921 agcgtgttca ggagtcaatt gccccaatga gccaccaatc aaccaaagct atcgtcatat
     4981 ttgccaccgt gtggcttgct gcgggcagtg ctgctgcaga ttggacatat cgctcaggaa
     5041 tcaacagcat cggcggccag cccggcaaag tcgccgagat caggagcagc aagtcgctca
     5101 accttaaatt tccgtataac ggcagcaatt tcggttcaat cattgttcga ccgaaacctg
     5161 gaccagaccc gagcgtgacc ttctcggtgg acatgggcca gttgacttgt gattcctaca
     5221 gtggctgctc gatcaacctg cgttttgatg atggcccgcc agtcgaattc agtgtcaggg
     5281 ggtcaaccgg gttcggttca aaccagcttc atttcaaaaa cgaaagtcgg tttgttgcat
     5341 tggccagccg cgccagccga atctatgtgc aggtatcaat gtaccaggca gggcgccaaa
     5401 cgctcgagtt caggtctgtg ataccgctgg tctgggactc aagaaaagtc actgagccat
     5461 tgcgaccgaa tgtgtgggaa cccagaaggc ccttggagcc gcaacgatca cattgaaagt
     5521 gccccgtgtc tgccgaaccc ttaagccagc gcagttggcc aggttgccag cgtgacgggg
     5581 cgcgccagcc tgccggataa ccgctttgaa aacaggtcaa ggtgcgccaa cgggctgttg
     5641 ccagcgaacg gaaaatgcga ttgccagcag ttcactgcct gacaactgac tgcgctaggc
     5701 agccaccggc tgcacctgca ccaggcagtc ataaaaggtt ggtgcagcac ccaggtcggt
     5761 caggcgctgg ctggtgagct ggttgacatt ggtgccgctc ggcccgagtt tgcgccacca
     5821 gacccccagc ccattgacaa cgccagggcg ggcgcgcgtg ccgacgttga cgcggcaacg
     5881 gtattcgccg cgctcattga acacccgcac catgtcgccc tgggcaatgc cgcgtgcgct
     5941 ggcatcactg gcgtgcatct ccagtagcgg ctcgctttcc atcgcctgca ggctgttcac
     6001 attgacaaag cttgagttca agaaattgcg tgccggtggc gagatcatcg ccaacgggta
     6061 agtggcagag gtgttggccg cttcgtaatt gggcacatgg tcgggcaggc catcttggcc
     6121 ccggtcagcc aggcgttggc tgaaaaactc gcactttcct gacggggttg gaaaattccc
     6181 ctgggcaaaa ggtgcctcag gtgtggccag ggtggcaaaa ccctgtgtca gcagggtatt
     6241 gaaatcaagc gcctcgccat aggccatacg gcacagcgcc tcatcgctgt cggcaaaaca
     6301 cgcatcgtca aaacccatgc gctggctgag ttcccggaaa atttgcgtgt tgggcttggc
     6361 ctgcccgagc ggtgcaatgg ccgggcggtt cagcaacacg tcggtgtgac cgtagctcag
     6421 gtgcacgtcc caatgctcaa gctgggtggt agcaggcagg atgtaatcgg caaagtcggc
     6481 ggtgtcggtc tggaaatgct ccagcaccac ggtaaacagg ttgtcgcggg caaaaacctg
     6541 caccaccctg gcggaatcag gggcaatcgc caccgggttg ctgttgtaca cgatcagcgc
     6601 ctcaatggcc gggccaaact cggcgctggc ggtgcgcaac aggtcgtcac caatggttgc
     6661 catattgatg ctgcgtgggc tgcggccagc cagcaggtca gggcgttcca gcaactggcg
     6721 ctgcaccggg aagttgcccg agcttgacag caacatgcca ccagcacgct ggcgccaagc
     6781 tcctgtcagc gctggcagca tggcaatcag gcgcaccgcg ttgccgccgc catgcacccg
     6841 ctgcagaccg tagttcatgc ggatggcggc tggctcgccg gatttgacgc tggcaccgta
     6901 atcgcgcgcc agtgacctga tttgcccggc atcaataccg cagacggtgg cggcgcgctc
     6961 aggcggccac tgcagggccc gctgttgcaa ggctggccag cccagcgtgt agcgcgccag
     7021 gtaatcgtga tcgagccagt cgtgggtaat caattgctgc atgatcgata gcgccaaagc
     7081 gccgtcggta ccgggcagca gggcaatatg ctcatggcat ttgtcggccg tctcgctctt
     7141 gcgcgggtcg atgcagatca gtttggcacc attgcgtttg gcctggttcg cgtagcgcca
     7201 gaaatgcagg ttgctcgaaa tcgagttgct gccccagatc aggatcagtt ttgattcggc
     7261 aaaaaattca acccgcatgc cagtcttgat accgagcgta tgcagcaacg cctcgccacc
     7321 ggcggtggcg caaatggtcc ggttcagcaa ggatgcgccg agtttgtgaa aaaagcgtgc
     7381 cgacatgctg tcaccctgca ccagccccat ggtgccgccg tagctgtagg gcaatatcgc
     7441 ctgtgggtcg cgccgggcaa tcgcgctcag gcgctgcgcg atgtcgaaca gcgcggcatc
     7501 ccaactgacc gcctcaaact gccctgcgcc ttttgggcca atgcgcttga gcggttgcag
     7561 gatgcgatca cggtgatagg tgcgctcagg gtaacgcgac accttggtgc acagcgcgcc
     7621 atcggtgtgc gggtgcgccg ggttgccctg caccttgatg gcgacgccgt tggctaccgt
     7681 ggtcagcatc gaacaggtgt cggggcagtc gtgcgggcag gctccgatga tttgccgggt
     7741 atcggcatca aagtgggcgt tgagatggtt catggcagca ggtgtcaggc agggtggatg
     7801 ggtttgggcg cattttgacg caaaccaagg catgtgcatt gggtggtcgc aatggccctg
     7861 gcggcaatcc gcgccattga tgctggcgct tggggcggtc tgtctggctc tgcggcacag
     7921 cctgcaggca ctttgcgcgg tacaggttca gccgccgtca cgcaccaccc ggcatgactc
     7981 gatgtcaaac gacacactgc caatggccag cgaagtctgg tctttcaagc ctgcaacaaa
     8041 ggcaaggaag cgtgctacgt cggcgaagtt gctggcgatg ccggttgaaa cgcgaatagc
     8101 ccccgcgctc ttgccgctgc gcgcctgcag tgcttgcgaa aaccgcagca ggttgatctc
     8161 gtcaccagcc tgtgcaatgc ctagccgcat ctcttcttcg cccaggtcct cggcagtttc
     8221 accggcaccg gggttgcaaa agcagccagt gcgcagcgag atgtgcgcct ggccggccag
     8281 ttcttcaacg cgccggtaat ccaccaggtg accatgaggg tcatagacat tcatggtgac
     8341 ggttgcgcca cgcatggtca tgttggccgg gccatacagg cgcaccatcg gctggccgtt
     8401 gctgtgcctg agcgccagca gttgggtgat cagccagccg gtcaggcagt tgacgcgggt
     8461 ttgaatggtc tcaatgccga cctgctgcag atggcgcagg ccaatttcca cggccggaat
     8521 acccaggtaa ttgagagtgc cgtcttcaaa gcccgcttcg ccatccgaca agacgtgcag
     8581 gcgggcttgt accgtggcaa agttgaccgt gccgccacca aaccatggac ggcgcagcgc
     8641 cttgagcgtg gcattgcgca ccagcaggca gccgacaccg gtcgggtagc caaacatctt
     8701 gtaaaaggac atcacgacaa agtccggccg cactgcgcgc agatcgagcc ggttggtcgg
     8761 cacaaaggcg gctgcgtcca gcagcacatg ccagccctgg gcctgcgccc gggcgaccag
     8821 ttccagcggg tgtttgacac ccgaaaaatt cgactgtgcc ggaaacgcca gcagattcgc
     8881 ctgcgtcggg tcggcctgcg ccaacagcgc atccatggcg ccggagtcaa tgcgcagttc
     8941 cgggcgggtc agtggtgcgt agctggccag cagccccttg gcccgtgcga attcgcgcat
     9001 gccattgacc gagttatggt tgtccgctgt caacagcagt ttgccgcagg gggcaaaagg
     9061 gtacgactca ccaacgagtt tgagcgcgcc agaggcgttg agtgtgaaga tggcggtgta
     9121 gtcaccggtg ccgttgaaat aggccagcac cgcagcccgc gcctgttcga cccggtgggt
     9181 tgttgccatt gagctcggac ttgccgaatg cgggttgcca agcacatggt cgctgagcat
     9241 ctgtgcgtgt tcatgcactt gggatgcggc atgcagcccg gcgccggtgt aatccagata
     9301 aacctcatcc tgggcatcga gtcggccata ttgggcagcg cgcaaatcat caagcgcacc
     9361 ggttgccgcg tagtccggat ggctggctaa aaattggtcg tatgcggtca tggatgacca
     9421 ttttaggagc ctgtcggact tgcggcggat cagctcagag ccgaccgcag ccgactcatt
     9481 gtgaactggc tttttgaccc gcaaccgggt gctgggtcgg caaatctgcg accaactggc
     9541 agcgcagggg ccggttctca tgatcgatgc gataagtccg tccgccgctt ggtctccaac
     9601 aagtcaagtc tttgcatacc atgcgaagaa ttcaaacatt caaagtcatt cgccgcgagg
     9661 gcgcgactcc tacacattca aataggcgcg gactgtttgt gtaggagtcg cgccctcgcg
     9721 acgaaggccg tccaattccg gcacgtctgg cttaggttga agttgagcaa tgcttgttga
     9781 gccaactggt gctggcatca cgcctgaagc gtccaaaaat ttgaccataa aaaccagcaa
     9841 tgctggcagg gctcaggtca gccggagtcg atccggatgg cat
//
LOCUS       DAOSLL010000094         9860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6641_length_9860_cov_5.011321, whole genome shotgun sequence.
ACCESSION   DAOSLL010000094 DAOSLL010000000
VERSION     DAOSLL010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9860
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6641_length_9860_cov_5.011321"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="PLB25_05840"
     CDS             <1..445
                     /locus_tag="PLB25_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41134.1"
                     /translation="SDELAAVGQGDVIDGLTRPLNIKPGSRLLVDGFDWLGGALARSR
                     LSSGVTLVPWDRHRAEKLLAAAIVCPCYEANAAFSVERLSHGLCAKALMGSLLNARNL
                     PKHGLAFANRFAGARPGYRIRYSNLADVTAWLEQQEFANSSLSQA"
     gene            complement(481..1488)
                     /locus_tag="PLB25_05845"
     CDS             complement(481..1488)
                     /locus_tag="PLB25_05845"
                     /inference="COORDINATES: protein motif:HMM:NF017497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 90"
                     /protein_id="HOE41135.1"
                     /translation="MNSPLQVSATSIDQFESAFSCKITTLPKNVALVGWSAKQQVWTI
                     APDSVTRHPTFSYLAPATLHFLHHELLPRLGHRSFWTLYCFYDGWRERNTFSNNYRWV
                     NPPKLDSAVEWRGAPGEIPMLSEQTFRIACYGAHRGDPSALLLPEAHYLARNHYIDLF
                     DLIRQHSMAWHEKRPQAIFCAGNHGECSNYFPPFVTGRPHPRQYLQQLVESTGLPVKV
                     YLGASIPQSEQLRYKYILDVDGYARTWDAWAWKMQSGSVVLSPDSPWESFFTRLFDPW
                     VHYVPMANDFSDLSDKLAWCTAHDADCQQIADQAKQRTRQVYSLGYVADKFAEEFQLV
                     F"
     gene            2256..2558
                     /locus_tag="PLB25_05850"
     CDS             2256..2558
                     /locus_tag="PLB25_05850"
                     /inference="COORDINATES: protein motif:HMM:NF015602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlaG"
                     /protein_id="HOE41136.1"
                     /translation="MDAVPAIAVRNEPEASAPAVNLRIPTTDELRKISQEMQRKIADM
                     SPELQFSVDKDSGRAVIKVTNRVTNELVRQIPSEEALELANDLAQFRKGLLLNQKV"
     gene            complement(2664..3887)
                     /locus_tag="PLB25_05855"
     CDS             complement(2664..3887)
                     /locus_tag="PLB25_05855"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOE41137.1"
                     /translation="MASGVLHDVSMGYQLLWGPQRKVAGVQLRMDVQSNTSVDARHLL
                     GTLGKMWRTDAPPLQLCTSSVALLADLLDMAPARQARVEVSDAQLQDPAIAARVVRAG
                     QRGLSLVWRGEPGTRFKAAYTSSFTQSILGLSTDEALMSLRIAKRSQGANLPADPASP
                     VRAGQIYEGVASQLLAEHVLGQQGAAALLGWPVEDVLYGYRQHRIQPGRAAIMRLIEA
                     IDADIAMEDIEQLLAQDALLAYRFLRYVNSAGAGLRREIDALRQGLMVLGLGRVKTWL
                     QEQLPKASSDRNLHPVRLGMTLRAQLMAETLDAGEGDALKRELHLCGLLSQIDQLLAE
                     PLTSALGSVPLPARVIAALVDHDGPYWPYLAIACALESPGTVSQSLCERHGIDQEAVN
                     QALLRTLCTATRPLH"
     gene            complement(3892..5205)
                     /gene="flgL"
                     /locus_tag="PLB25_05860"
     CDS             complement(3892..5205)
                     /gene="flgL"
                     /locus_tag="PLB25_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HOE41138.1"
                     /translation="MATLSTARLGSANTYDNAIRIVTARQAALSELQENLTSGKRVVR
                     ASDDPTSAAIAERALTRISRIATDQRALESQRTSIATTESTLGDVTDALQRFRELVVS
                     AGNGVHSAAERRTIAQEMQGLRDQVFALANTKDQNGLPLFNALGSALTPFVGPQATAP
                     DYSFNGLPGQSAASAVTIPFTLDGDNAFMHQPARDQAFNAIVTNTVDGLISTDRTLTT
                     DNVVMTNQATVSATAAAAQAVAPPNNVPYPKYTVAFTAVDTTTVPGTTTATYTTTEVP
                     PVSGVIAAVTVSYPTNTSITVATLPGLSFNITGTPKVNDTITIDSSASVFSVLDDAIR
                     DIGEAGNNNASAQAVSQALHNIDIGLARVSNVRGQAGELLNRADRISGNQEKRSIQME
                     ENRSRAEDLDMIKGISDFSNQQTGYSAALQTYAQIQKLSLFNFIS"
     gene            complement(5229..7235)
                     /gene="flgK"
                     /locus_tag="PLB25_05865"
     CDS             complement(5229..7235)
                     /gene="flgK"
                     /locus_tag="PLB25_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HOE41139.1"
                     /translation="MSGLLNVATRALVANQTVLQTTGNNIANVNTPGYSRQSAVLETV
                     PGQFTGSGYIGKGVNVATIQRNYSEFLTRQSTLAGAISSGDTARADKLRQLETLFQGG
                     ANGLGAAVSDMLNAFSDVASAPTDLTARTVALTRVNETALRMRSTSASLDELQASVTQ
                     ELNNKINSINTLAAGIASVNDKIARAQGSGQPPNDLLDQRDQLVRELNQYIQTTSIPA
                     TDGTVGIFIGGSQSLVLGTSVASVRLTSDEFGDPLKAKLTLIRNGQSAVMDENVLGGG
                     QVPGMLRFQNADMAEARNLLGRYTLAVSTSMNEQHKLGLDLDGNVGGNLFTPTTDAAN
                     AFGTATVLKPTPANSTNYNLTLFIPDLSDVTKFEASDYEVSFTSNTAGNITRRSDGTP
                     IQFTYAAATGTFSFQTATDPAAGPYTGTAFDGLGLSTVALTPVVAGDRFLLKPFSTSA
                     SNISSEFSSPRALAVASPVAGRMGTTNTGSLQLASLSARDNPPAAVPVTLSFTGANSY
                     TRSDTGATVYTYTSGQAIEGSVPVTTPLSEWSLVLQGVPKAGDTFTVEAMPATMRNLN
                     SANASAMMNLRDVAMFDGSAMTDGYASLISKIGIRAQSANYTAEVSGSIAANLEKDRT
                     GVSGVNLDEEAAKLLQFQQAYQASAKVIQIAQGIFDTLIQTMSR"
     gene            complement(7232..8155)
                     /gene="flgJ"
                     /locus_tag="PLB25_05870"
     CDS             complement(7232..8155)
                     /gene="flgJ"
                     /locus_tag="PLB25_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HOE41140.1"
                     /translation="MSLNPVSTAANALAADASSLGRLKMQAGQGTPEAIKETAKQFES
                     LFMRELIKSMRQATMKSGLLDNPGSDLGTDLLDQQFAVQMSGKPGGLSDLIAAQLSRQ
                     MGVANAQSTGAGTTTGQATLPLSSLQKASSLSVYGKNAVKPTTSQSNFVEKHTEAAIR
                     IEKESGIPASYMVGQAGHETGWGQFEIKQRGGKPSYNLFGIKAGAGWQGKVAEVTTTE
                     YTNGVAQKTVAKFRAYDSYDESFRDYAKLITQSPRYAKVTAQTGSALAFATSLQKAGY
                     ATDPNYAAKLSRAIETTQRLQQRTQVMAQSV"
     gene            complement(8155..9315)
                     /locus_tag="PLB25_05875"
     CDS             complement(8155..9315)
                     /locus_tag="PLB25_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HOE41141.1"
                     /translation="MHATANTHITTSSTRYATAVLSVLLVFLASLGLAGNAQAARIKE
                     VAAVEGVRSNQLTGFGLVVGLDGTGDQTTQMPYTSQGISNYLQQLGITLTSDAVSKLQ
                     LKNVAAVLVTAQLPAFARPGQAIDVNVSSVGNAKSLKGGMLISTPLKGADGEIYAVAQ
                     GNLVVAGAGAAAGGSKVQVNHLSAGRIPNGAQVERVVATPLQEGASITLGLEASDFQT
                     ARRVAQAINTKFGAGSAKAMDGRTIRVKAPVEPDARVTFMAELEELQLESSVPSAKVV
                     INSRTGSIVMNQSVSLGACAVAHGNLSISISTTPSVSQPNALSGGQTTVTEKSDIQIK
                     AEPGMLIQLPASPQLSEVVRALNSLGATPQDLLAILQAIKAAGALNAELEVI"
     gene            complement(9350..>9860)
                     /locus_tag="PLB25_05880"
     CDS             complement(9350..>9860)
                     /locus_tag="PLB25_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HOE41142.1"
                     /translation="PAFEDVRARLVGDIVTIQIVEKVSASQVSSSTANRTSSGSASIS
                     AFPLISAADLARLNAGIGTDSKSDFSGKGGTESANTFYGTITTTVVEVLPNGHLVVAG
                     DKQIGINQNVDVMRFSGTVDPRLIQPGNLINSNQVANARMESKGRGAQAEAQTVGWLT
                     RFFFNALPF"
BASE COUNT         1904 a         2741 c         3037 g         2178 t
ORIGIN      
        1 gtctgatgag ttggccgcgg ttgggcaagg tgatgtgatc gacggcctga cccgcccctt
       61 gaacatcaag ccgggcagcc ggttgttggt ggatggattt gactggttgg gtggcgcgct
      121 ggcccggtcg cgattgtcca gcggtgtcac tctggtgcct tgggatcggc atcgcgccga
      181 gaagctgctt gccgccgcca ttgtttgccc gtgctatgag gccaatgctg ctttcagcgt
      241 tgagcgcctg agccatgggc tttgtgccaa agcgctcatg ggtagcctgt tgaatgccag
      301 gaacctgccc aagcacggtc tggcttttgc caaccgcttt gcaggtgccc gcccaggcta
      361 ccggatcagg tattccaatc ttgcggatgt gacggcctgg cttgagcaac aagagttcgc
      421 caatagcagc ttgtcgcagg cttagtcgcc agcggacgtt ccgatttggc acggctgcgg
      481 tcagaaaacc aactgaaact cctcggcgaa tttgtcggcg acatagccga gcgagtacac
      541 ctgccgggtg cgctgtttgg cctgatcggc gatctgctgg cagtcggcat catgcgcggt
      601 gcaccaggct agtttgtcag acaggtccga aaagtcgttg gccatcggca cgtagtgcac
      661 ccatggatca aataggcggg taaaaaaaga ttcccagggt gaatctggcg acagcacgac
      721 tgaaccgctt tgcattttcc aggcccatgc gtcccaggta cgcgcatagc catccacgtc
      781 gaggatgtat ttgtatctga gttgctcgct ttgggggatg gacgctccga ggtacacctt
      841 caccggcaaa cccgtgcttt ccacaagctg ctgcaggtac tgccttggat gcggtcggcc
      901 ggtaacaaag gggggaaagt aattgctgca ttcaccatgg ttgccagcgc aaaaaatggc
      961 ttgcggcctt ttttcatgcc atgccatcga atgctgacgg ataaggtcaa aaagatcgat
     1021 gtagtggttt ctggccaggt aatgggcttc gggcagcagc aacgcgctgg gatcgccccg
     1081 gtgagcgcca tagcacgcga tcctgaaggt ttgctcgctc aacatgggta tttcgccggg
     1141 tgcgccacgc cattcgacgg cactgtccag ctttggcggg ttgacccagc gatagttgtt
     1201 tgaaaaggtg ttgcgttccc gccagccatc gtaaaagcaa tacagggtcc aaaaggaacg
     1261 atggcccaat ctggggagca attcgtggtg cagaaaatgc agggttgccg gtgccagata
     1321 actgaaagtc gggtgccggg tgacgctgtc tggtgcaatt gtccagacct gttgttttgc
     1381 tgaccaaccc acaagtgcca cgttttttgg cagcgtcgtg atcttgcagg aaaacgccga
     1441 ctcaaactga tcgattgatg ttgcgctgac ctgtaaggga gagttcatgg gtatggggtc
     1501 gggtacaccg gtgtttcagt ggcgctggcg tgtcgcctgg actggttgac cgggttcagg
     1561 gcgagtgggt gaaattttag aagaaattcg ccttcagact aattggaata agcgctgagc
     1621 gctataaatt atatagtgat atgagtcgct gactcaacgc cagacttgct ggcctatggt
     1681 agatgatggc acatccagac ttgttgggct ggccagtttc agttccagcg cccgaatggc
     1741 tgagaaccca ttggcgggca gttcgatgca ggacaggcca aaaaatcgca cttaagtaaa
     1801 gcaaaccccg gccgataaac aacccatgcg gggaaattta agtggcctcg ctggtgctgc
     1861 gagacatccg cagggctggt ttactccaac tttttacttg tcagaggctc aaatggtagt
     1921 cagttctatt tactcaaatg cggtgcaatc ggccgcccgg gctgcgccaa gtgctactga
     1981 tatggcctca caaagccgtg aggctgtgcg caacgatggt gactggacga tgcacaaggc
     2041 gtgttgccgt ccaatgtggc caagccggtc acccgtaccc tgggtcaact tattggcgca
     2101 aatgtgaatg ccacggcctg atcgctgacc cagcccttgt agttaacgca gagttaccca
     2161 tcatggttac ttccaattca gtcactcttg tgacaggggt aaagacctca ccgtcaagcc
     2221 cgttgccggc caaggcaggc agttctgacc ggttgatgga cgcggtgcct gccattgcgg
     2281 tgcgcaacga gccggaggcg tcagccccgg cggtaaatct gcgcattcct acaaccgacg
     2341 agttgagaaa aatctcacag gaaatgcagc gaaagatcgc cgacatgtcg cctgagttgc
     2401 agttttcggt ggacaaggac agtggccggg cggtgatcaa ggtgacaaac cgcgtaacca
     2461 atgagcttgt tcgacaaatt ccctctgaag aagccttgga attggccaac gatcttgcgc
     2521 agtttcgaaa agggttgctg ttgaatcaaa aggtctgaga tttcagaccc attcatgccg
     2581 ccagcccaat cagcacctgc gtgacaagct attaatagca tagcgtcatg tttggttttc
     2641 ggatcaaacc ggcctttgca ccatcagtgc agcggtcggg tcgctgtgca caaagtccgc
     2701 agcagtgcct ggttcacagc ttcctggtca attccgtggc gctcacacaa ggactggctg
     2761 accgtgccgg ggctctccag tgcgcacgca atcgcgagat acggccaata gggaccgtcg
     2821 tggtcaacca gtgccgcaat gacccgcgct ggcagcggca cagagcctag tgcgctggtc
     2881 agtggttcgg ccagcaattg gtcaatttgt gacaacagac cacacaggtg caactcccgc
     2941 ttgagcgcat cgccttcacc cgcgtccagc gtttctgcca tcaactgcgc gcgcagtgtc
     3001 atgcccaggc gcaccgggtg caggttgcgg tcactgctgg ctttgggcaa ttgttcttgc
     3061 aaccaggttt tgacacgtcc gaggcccagc accatcagac cctggcgcaa ggcatcaatt
     3121 tcacggcgca ggcccgcacc agcggagttg acataacgca gaaaccggta agccagcaag
     3181 gcatcctgag ccaaaagttg ctcgatgtct tccatcgcga tgtcggcatc aatggcctcg
     3241 atcagccgca tgatggcggc gcgaccgggt tgaatgcggt gttggcgata gccatacagc
     3301 acgtcttcca ccggccagcc cagcagggcg gcggctccct gctggcccag cacatgttcg
     3361 gccagcagtt ggctcgccac accctcgtag atttgaccgg cacgtaccgg gctggccgga
     3421 tcggctggca agtttgcgcc ctggctgcgc ttggcgatgc gcaggctcat caacgcctcg
     3481 tcggtactca agcccaaaat gctctgtgtg aaactgctgg tgtaagcggc cttgaaccgg
     3541 gtgcccggct cgccacgcca taccagcgag agcccgcgct gcccggcccg caccacgcgc
     3601 gcggcgatgg cagggtcttg caactgtgcg tcactcacct caacgcgtgc ctgcctggca
     3661 ggtgccatat cgagcaaatc agccagcagg gcgactgatg atgtgcaaag ttgcaggggt
     3721 ggcgcgtcag tgcgccacat tttgccaagc gtgccaagca ggtggcgcgc atcaacgctg
     3781 gtattggact gcacatccat tcgcaactga acgcccgcca ccttgcgctg tggcccccac
     3841 agcaactggt agcccatcga gacatcgtgc aagacaccgc tggccatggc atcaactgat
     3901 gaagttaaac agcgacagct tctggatctg cgcgtaggtt tgcagtgccg ccgaatagcc
     3961 ggtttgctgg ttactgaagt ccgagatgcc tttgatcatg tccaggtctt cagcgcgcga
     4021 gcggttctct tccatttgaa tgctgcgctt ttcctggttg cctgaaatgc ggtcggcgcg
     4081 attcagtagc tcgcctgcct ggccgcgcac gttggaaacg cgcgccaaac cgatgtcgat
     4141 gttgtgcagg gcttggctaa ccgcctgggc tgaggcgttg ttgttgccag cctcgccgat
     4201 atcgcgaatc gcgtcgtcga gcacgctgaa cacgctggcg cttgagtcga tggtgatggt
     4261 gtcgttgacc ttgggcgtac cggtgatgtt gaaggacagt ccaggaaggg ttgctaccgt
     4321 gatgctggta ttggtcggat aactgacggt gactgccgca ataacaccgg ataccggtgg
     4381 aacttcggtg gtggtgtagg tggcggtggt ggtgccgggc acggtggtgg tgtccactgc
     4441 ggtgaatgcc accgtgtatt tggggtaggg cacattgttt ggtggggcaa cggcttgcgc
     4501 tgcggcggcg gtggctgaga cggttgcctg gttggtcatc accacgttgt cggttgtcag
     4561 agtgcggtcc gtgctgatca aaccatcgac ggtgttggtg acgatggcat taaacgcctg
     4621 gtcgcgtgcg ggctggtgca tgaatgcgtt gtcgccgtcc agggtaaatg gaatggtgac
     4681 ggcgctggcg gctgattggc cgggcaggcc gttaaagctg tagtccggtg ccgttgcctg
     4741 cggcccgaca aaaggggtca gggcgctgcc gagcgcatta aacaagggta gcccgttttg
     4801 gtctttggta ttggccaatg caaaaacctg gtcgcgcagg ccttgcatct cttgagcgat
     4861 ggtgcgtctt tcggcagcgc tgtgcacgcc gttaccggcg ctgaccacca gctcacgaaa
     4921 gcgctgcagg gcatcggtca catcgcccaa ggtactctcc gtcgtggcaa tggaagtgcg
     4981 ctgtgactcc agcgctcgct ggtctgtggc aatgcggctg attcgggtca aggcgcgctc
     5041 ggcaatggcg gcactggtgg ggtcgtcact ggcgcgaacc acccgcttgc ccgaagtcag
     5101 gttttcctgc agttcagaca aggccgcctg ccgcgcggta acgatgcgta tcgcattgtc
     5161 gtaggtgttg gctgaaccga gccgtgccgt ggatagtgtt gccatgatga ggactcctgc
     5221 tcgtgatgtt agcgactcat ggtctgaatc agggtgtcaa aaatgccctg cgcgatctga
     5281 atcacttttg ccgaggcttg ataggcctgc tgaaactgca acagtttggc cgcttcttcg
     5341 tccagattca cacccgagac cccggtgcgg tccttttcca gattggcggc aattgagccc
     5401 gacacctcag ccgtgtaatt ggcgctctgg gcgcggatac cgatttttga gatcaggctg
     5461 gcatatccgt cggtcatcgc cgagccgtca aacatggcga cgtcgcgcag gttcatcatg
     5521 gcagaggcgt tggcggagtt gaggttgcgc atggtggctg gcatagcctc aacggtgaag
     5581 gtatcgccgg ctttgggcac accttgcaac accaaagacc attcgctcag cggcgtggtg
     5641 acagggacgc tgccttcaat ggcttgacca gatgtgtagg tgtaaacagt cgcaccagtg
     5701 tcgctacgtg tgtagctatt ggcacccgtg aaggacaatg tgaccggcac cgccgctggg
     5761 gggttgtcac gtgctgacag cgaagccagt tgcaggctgc ctgtattggt tgtgcccatt
     5821 ctgcccgcca ccgggctggc cacggccagc gcgcgtgggc tggaaaactc ggaactgatg
     5881 ttgctcgccg aggtgctgaa gggtttgagc aaaaagcggt ctccggcgac aaccggtgtc
     5941 aatgcaacag tggaaagacc caagccgtca aacgccgtac cggtgtaagg tccggctgct
     6001 ggatcggttg cggtctgaaa tgaaaacgtg ccagtggcag cggcataggt gaactggatg
     6061 ggcgtcccat cagagcggcg tgtgatgttt ccggctgtgt tggaggtgaa actcacctcg
     6121 tagtccgaag cttcaaactt tgtgacgtca ctgagatcag gaatgaacag tgtcaggttg
     6181 tagttggtgc tgtttgcagg agtcggtttc aacacagtcg cagtaccaaa cgcattcgcc
     6241 gcatccgtcg tcggcgtgaa taaatttccc cccacattgc catccagatc cagcccaagc
     6301 ttgtgctgct cgttcatgct ggtgctcacc gccagggtgt agcgtccgag caaattgcgg
     6361 gcttcagcca tgtccgcgtt ctggaaccgc aacatccccg gaacctgccc gccacccagg
     6421 acgttttcat ccatcactgc gctttgaccg ttgcggatca gggtcaactt ggctttgagg
     6481 ggatcaccaa actcgtcgct ggtcagcctg accgatgcca ccgaggtgcc cagtaccagc
     6541 gactgactgc cgccaatgaa aatgcccacg gtgccatcgg tggccgggat cgaggtggtc
     6601 tggatgtact ggttaagctc gcgcaccagt tgatcgcgct ggtcgagcag gtcgttgggc
     6661 ggctgtccac tgccctgggc gcgcgcaatt ttgtcattca ccgaggcaat gccagcagcc
     6721 agcgtgttga tggaattgat cttgttgttg agttcctgcg tcacgcttgc ctgcagttca
     6781 tcaagactgg cagaggtgct gcgcatacgc agggcggttt cattgactcg ggtcaaggcc
     6841 acggtgcgtg cggtcaggtc ggtcggtgcg cttgccacat ctgaaaacgc atttagcatg
     6901 tcgctcaccg cagcacctag gccgttggca ccaccttgaa acagggtctc aagctgtctt
     6961 agcttatcag cgcgcgctgt gtcgcctgag ctgatggctc cagccaaggt tgactggcgg
     7021 gtcaggaatt cgctgtaatt gcgctgtatt gtcgcgacat tcacaccttt gccgatatag
     7081 ccactgccgg tgaactggcc tggcacggtc tccagcaccg ccgactggcg tgaatagccg
     7141 ggggtgttga cattggcaat gttgttgcca gtggtttgca agaccgtctg gttggccacc
     7201 aaggcccgcg tcgcaacgtt cagcaggccg ctcatacgct ttgcgccatg acttgcgtgc
     7261 gctgctgcag acgctgcgtg gtttcaatgg cacggctcag cttggcagca tagtttggat
     7321 cggtcgcata gccggctttt tgcaagctgg tggcaaacgc cagtgccgag ccggtttgcg
     7381 cggtcacttt ggcatagcgg ggcgactggg tgatcaactt tgcgtaatcg cgaaacgact
     7441 cgtcataaga gtcataggcg cgaaattttg cgaccgtttt ctgtgccacg ccattggtgt
     7501 attcggtggt ggtgacctcg gctaccttgc cctgccagcc ggctccggcc ttgatgccaa
     7561 acaggttata ggacggcttg cccccgcgct gcttgatctc gaactggccc cagcctgttt
     7621 catggccggc ctggcccacc atgtagctcg ccggaatgcc cgattctttc tcgatccgga
     7681 ttgccgcctc ggtgtgcttt tcgacaaagt tgctttgact ggtggtcggt ttcacggcgt
     7741 ttttgccata aacggacagc gacgaggcct tttgcaagct gctcaagggc agcgtggctt
     7801 gaccggtggt ggtgccagcg ccggtggact gcgcattggc aacacccatc tggcgcgaca
     7861 actgggctgc aatcagatcc gacaagccgc cgggtttgcc cgacatctgc acggcaaact
     7921 gttggtccag caggtcagtg ccgaggtcgc tgccagggtt gtcgagcagg ccggacttca
     7981 tggtggcctg gcgcatgctt ttgatcagtt cacgcatgaa cagcgactcg aactgcttgg
     8041 cagtttcctt gatggcctcg ggcgtgcctt gcccggcctg catttttagc cgacccagcg
     8101 agctggcatc ggcagccagt gcgttggccg cagtgctgac tggattcagg ctcatcagat
     8161 cacctccagt tccgcgttga gtgcgccggc ggccttgatc gcctgcaaaa tcgccagcag
     8221 gtcctgtggc gtggcaccga gcgaattcag cgcccgcacg acttccgaga gttggggcga
     8281 tgctggcaac tgaatcagca tgcctggctc ggccttgatc tggatgtcgg acttttcagt
     8341 caccgtggtt tgtccgccag acagcgcatt gggctggctg acgctcggtg tggtgctgat
     8401 gctgatggac aggttgccgt gagccaccgc gcaggctccc agactcacag actggttcat
     8461 cacgatcgag ccggtacgcg agttgatcac caccttggcc gatggcaccg acgactcaag
     8521 ctgcaactct tccagttctg ccataaaggt gacgcgggca tcgggctcaa ccggggcctt
     8581 gacgcggatg gtgcgcccgt ccattgcttt cgctgatccg gcaccaaatt tggtgttgat
     8641 ggcttgcgcg acgcgccgcg cggtttgaaa gtccgaagct tccagcccga gcgtgatgct
     8701 ggcaccttct tgcaaggggg tggccacgac acgttcgacc tgcgctccgt tggggatgcg
     8761 accggcgctc aggtggttca cctgcacctt gctgccgcca gcggcagcac cggcgccggc
     8821 aaccaccaga ttgccctggg caacggcata gatctcgccg tcagcgcctt tgagcggtgt
     8881 cgaaatcagc atgccaccct tgagcgactt ggcgttgccc accgacgaca cattgacatc
     8941 gatggcctgc ccaggacggg caaaagcggg caattgggcc gtgacaagta cggcggcaac
     9001 gttcttgagc tgcaattttg aaacggcatc gctcgtcagc gtgatgccaa gctgctgcaa
     9061 atagttgctg atgccttgcg atgtgtatgg catctgggtg gtttggtcac cggtgccgtc
     9121 cagccccacc accagcccga agccggtaag ctgattgctg cgcacgcctt ctactgccgc
     9181 cacttccttg atgcgggctg cctgcgcatt tccggcaagc cccaggctgg caagaaaaac
     9241 cagcaacacc gacagcaccg cagtggcata gcgggtcgat gatgttgtga tgtgtgtgtt
     9301 ggcagtggcg tgcatgtttt gagacccttc ggcacgattc tgcgcaactt tagaagggta
     9361 aagcgttgaa aaagaaccgt gttaaccagc caactgtttg tgcttcagct tgtgcgccgc
     9421 gacctttgga ttccatccgg gcgttggcga cctggtttga gttgatcaag ttgccgggtt
     9481 gaatcaggcg cgggtcaacg gtgccagaaa aacgcatgac atcgacgttc tggttgatgc
     9541 cgatctgctt gtcgcctgcc accaccagat gaccattggg caacacctcc acgacagttg
     9601 tcgtgatggt gccataaaag gtattggcac tctctgtgcc gcccttgcca gagaaatcac
     9661 tttttgagtc ggtgccgatg ccagcattca agcgtgccag atcggctgcc gagatcaggg
     9721 gaaacgcgct gatgctggcg gaacctgagg atgtgcggtt ggcggttgaa cttgagacct
     9781 ggctggcaga gactttttca acgatttgaa tcgtgacgat atccccgacc agtcgcgcgc
     9841 gcacgtcctc aaaggccggg
//
LOCUS       DAOSLL010000095         9835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6664_length_9835_cov_4.116667, whole genome shotgun sequence.
ACCESSION   DAOSLL010000095 DAOSLL010000000
VERSION     DAOSLL010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9835
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6664_length_9835_cov_4.116667"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="PLB25_05885"
     CDS             <1..151
                     /locus_tag="PLB25_05885"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057832.1"
                     /note="The point of entry for the majority of electrons
                     that traverse the respiratory chain eventually resulting
                     in the reduction of oxygen; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HOE41143.1"
                     /translation="YSYSVVRGCDRIVPVDVYVPGCPPTAEALIYGILQLQRKIRRTQ
                     TIARV"
     gene            162..779
                     /locus_tag="PLB25_05890"
     CDS             162..779
                     /locus_tag="PLB25_05890"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOE41144.1"
                     /translation="MTTFSIRPEDIQTAVEAALGPMCMHVSIDFDQVTVVVSAEDYRA
                     AAVALRDNPACRFEQMMDLCGVDYSEYPSGTSSGLRFCVVLHLLSVSLNQRLRLKVFV
                     PDDSAPVISSVTDVWSSANWFEREAFDLYGIVFEGHDDLRRILTDYGFIGHPFRKDFP
                     LSGHVEMRYDAEQKRVVYQPVTIEPREITPRVIREENYGSLQKAV"
     gene            783..2036
                     /locus_tag="PLB25_05895"
     CDS             783..2036
                     /locus_tag="PLB25_05895"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014616656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HOE41145.1"
                     /translation="MAEIKNYTLNFGPQHPAAHGVLRLVLELDGEVIQRADPHIGLLH
                     RATEKLAESKTYLQTLPYMDRLDYMSMMCNEQAYCLAIEKMLGLDVPLRAQYIRVMFA
                     EITRLLNHLLWVGAHAIDCGAMTVMLFAFREREDLLDMYEAVSGARMHAAYLRPGGVY
                     RDLPDTMPRHAPNKIRSAKSTEALNRNRSGSLLDFIDDFTQRFPTYLGEYHTLLTDNR
                     IWKQRTVGIGVVTPERALNLGFSGPMLRGSGFAWDLRKSQPYEVYDRLDFDIPVGKTG
                     DTYDRYLVRMEEMKQSNRIVKQCVDWLRVNPGPVITSNRKVAPPDRESMKSNMEDLIH
                     HFKLFTEGFCVPEGETYAAIEHPKGEFGIYIISDGANKPYRMKIRPPAFVHLAALDEM
                     GKGHMIADAVAIIGTMDIVFGEVDR"
     gene            2033..2521
                     /gene="nuoE"
                     /locus_tag="PLB25_05900"
     CDS             2033..2521
                     /gene="nuoE"
                     /locus_tag="PLB25_05900"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HOE41146.1"
                     /translation="MISEESKTRFAREVAKYPVGQKQSAVMACLAIAQQEVGYVSEEV
                     EIEIAQYLEMPVMAVHEVTTFYNMYNQHPVGRFKLNVCTNLPCQLRDGVNALKYLEKK
                     LGIAMGETTPDGLFTLQQSECLGACADSPVILVNDRSMCSFMTTEKLDQLVDGLRAAG
                     DQ"
     gene            2518..3873
                     /gene="nuoF"
                     /locus_tag="PLB25_05905"
     CDS             2518..3873
                     /gene="nuoF"
                     /locus_tag="PLB25_05905"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HOE41147.1"
                     /translation="MMKAQQILSQFRSAGIETCFHGRHIEPQILAELNGSNWRLKDYE
                     GRGGYQALRKILGKDGSAGMTQDQVIATVKESALRGRGGAGFPTGLKWSFMPRQFPGH
                     KYLVCNSDEGEPGTCKDREILEFNPHIVIEGMAIAAYAMGTAVGYNYIHGEIFSTYDR
                     FEEALEEARAAGLLGDNILGSSFCFQLHAAHGFGAYICGEETALLESLEGKKGQPRFK
                     PPFPASFGLYGKPTTINNTETFAAVPWIIRNGGAAYVACGKPNNGGTKIYSVSGDVER
                     PGNFEVPMGTPFAKVLELAGGVRASRKLKAVIPGGSSSPVLPAAIMMECTMDYDSIAK
                     AGSMLGSGAVIVLDDSRCMVKSLQRLSYFYMHESCGQCTPCREGTGWLWRLVDRIESG
                     QGRASDMDLLDSVAGDIQGRTICALGDAAAMPVRAMLKHFRPEFEYHVEHKTCMVPAY
                     V"
     gene            3886..6024
                     /gene="nuoG"
                     /locus_tag="PLB25_05910"
     CDS             3886..6024
                     /gene="nuoG"
                     /locus_tag="PLB25_05910"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HOE41148.1"
                     /translation="MIEIELDGNKVDIEQGSMIMHAAEKAGTYIPHFCYHKKLSIAAS
                     CRMCLVDVEKMPKPMPACATPVTQGMVVHTKTEKAVKAQKSVMEFLLINHPLDCPICD
                     QGGECQLQDLAVGYGGTSSRYEEEKRVVFHKDVGPLISMEEMSRCIHCTRCVRFGQEI
                     AGVMELGMSHRGEHAEIETFEGRSIDSELSGNMIDICPVGALTSKPFRYSARTWELAR
                     RKSVSPHDSTGANLIVQVKNNKVMRVVPLENETVNECWIADRDRFSYEALNGDERLTT
                     PMLKQGGEWKAVDWKIALEYVAHGLQSIKRAHGAEAVGALVSPHSTLEELYLAGALVR
                     GLGSQNIDYRLRNAAFETEPKGARYLGMTIADLSHLQRVLVVGSNLRKDHPLFAQRIR
                     QAVRGGCVVTALSARPMDWAMTLGPVDIVPASGWVRALANIAAAIAHAKNVPAPQSGQ
                     ATDVATAIADSLLSGERKAVLLGNAAAHHSQADDLLALSLWIGAQTGASVGYLTEAAN
                     TVGAQLVGALPGPVGFNAGQMLAGALRATLLLNTEPQFDAANGEAAVQGLKQCEMVVT
                     LSPFKANLEISDVLLPIAPFTETPGTFVNAEGLIQSFHAVVKPLGEARPGWKVIRALA
                     NILNLPGFDFDSAQDVLLHLHADNAGIVPARVPASRLSNECGVVSVGQVSQLEPCVAT
                     IYQLDGIVRRSPSLQLTADARVADTLEVTA"
     gene            6021..7103
                     /gene="nuoH"
                     /locus_tag="PLB25_05915"
     CDS             6021..7103
                     /gene="nuoH"
                     /locus_tag="PLB25_05915"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HOE41149.1"
                     /translation="MIESIFTFGQGLVGAGWWTGLAWPTIWALIKVVLVLMLVLTAVT
                     YATLWERKLLGWVQIRLGPNRVGPWGLLQPIADALKLMTKEIVRPTASDKFLFYLGPV
                     LTVAPALAAWAVIPFGPEIAAANLNAGLLFLMAITSMEIYGVIVSGWSSNSKYAFLGA
                     LRASAQMVSYEIAMGFCLLIVLMVSASLNLSDIVMGQGKGYFAQMGLSFLSWNWLPLL
                     PVFVVYFVSGLAETNRTPFDVIEGESEIVAGHMVEYSGMSWAMFQMAEYASIWLISIL
                     ATIMFLGGWLSPVDSVFFNWIPGWIWLGIKTFGMMTLFIWVRVTFPRFRYDQIMRLGW
                     KIFIPVTLVWLVVIGAWMQTPWNIWK"
     gene            7115..7648
                     /gene="nuoI"
                     /locus_tag="PLB25_05920"
     CDS             7115..7648
                     /gene="nuoI"
                     /locus_tag="PLB25_05920"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HOE41150.1"
                     /translation="MITPTSNSGENCSVSVFSLKDFLSSFFLVELLKGMRLTGKYAFR
                     SKITLEYPEEKTPLSPRFRGLHALRRYDNGEERCIACKLCEAVCPAMAITIESEIRAD
                     GSRRTSRYDIDLTKCIFCGFCEESCPVDSIVETNILEYHGEQRGDLYFTKEMLLAVGD
                     RFEPEIAANKMADARYR"
     gene            7674..8333
                     /locus_tag="PLB25_05925"
     CDS             7674..8333
                     /locus_tag="PLB25_05925"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HOE41151.1"
                     /translation="MSVTTGLFYFFSVVLLFAAFRVITARNPVYAVLYLVLTFFQASM
                     IWMLLKAEFLSLSLVLVYVGAVMVLFLFVVMMLDVNIESLRIGFWKHFPLAAVVGIFI
                     SLEMSAVLMGGFRVSADPHAANSLASVGIAVSNTRELGKLMYTEYLYPLEVAAALLLV
                     AMISAIALTLRHRKESKAINAANQVQVKSRDRMTVVKVVATKPALPLTEPDAPSAGSK
                     V"
     gene            8330..8638
                     /gene="nuoK"
                     /locus_tag="PLB25_05930"
     CDS             8330..8638
                     /gene="nuoK"
                     /locus_tag="PLB25_05930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HOE41152.1"
                     /translation="MTLTLGHFLSLGAILFALSVIGIFLNRRNLIVLMMAIELMLLAV
                     NTNFVAFSYYLGDLNGQIFVFFVLTVAAAEAAIGLAILVLLFRSKSNISVDELDSLKG
                     "
     gene            8644..>9835
                     /gene="nuoL"
                     /locus_tag="PLB25_05935"
     CDS             8644..>9835
                     /gene="nuoL"
                     /locus_tag="PLB25_05935"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOE41153.1"
                     /translation="MSQTLSVSTLLAIPLAPLIGAIAAGVWGTKFGGNVIGRRLSHTL
                     AILGVLISFVLSAGVLKSVAIDGARFNETIYTWMVLGGLKMEIGFLVDGLSAMMMCVV
                     SFVSLMVHIYTVGYMEEDEGYNRFFAFISLFTFSMLMLVMSNNMLQLFFGWEAVGLVS
                     YLLIGFWFNKPSAIFANMKAFLVNRVGDFGFILGIGLLVAYTGSLNYSEVFAKSVEIS
                     KLTLPGTDWLLVTVICICLFIGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTA
                     GIFMVARMSPLFELSDAALNFILVIGGITALFMGFLGIIQNDIKRVVAYSTLSQLGYM
                     TVALGASAYSVAVFHLMTHAFFKALLFLAAGSVIMGVHHNQDIRWMGGLRKYMPVTWI
                     TSLLG"
BASE COUNT         2069 a         2238 c         2819 g         2709 t
ORIGIN      
        1 ttacagttat tccgtggtgc ggggttgtga tcgcattgtt ccggtcgatg tttatgtacc
       61 aggctgtcct cctactgctg aggcgctgat ttatggcata ttgcaactgc aacgcaaaat
      121 ccgtcgcacg caaaccatcg cgcgggtttg aaggctacag catgacaaca ttttccattc
      181 gacctgaaga catccaaacc gctgtcgagg cggcccttgg tccgatgtgc atgcatgttt
      241 caattgattt tgatcaggtg actgtggtcg tttcagcgga ggactaccgg gctgcggctg
      301 ttgcgcttcg tgacaacccc gcttgccggt tcgagcagat gatggacttg tgcggtgtgg
      361 attattctga gtatccctct ggcacttcaa gtggtttgcg gttttgtgtt gtgcttcact
      421 tgttgtctgt tagcctgaat cagcgccttc gattgaaagt gtttgttcct gacgactctg
      481 cgcctgtgat ttcaagtgtg acggatgtgt ggagttcggc gaactggttt gagcgcgaag
      541 catttgactt gtatggcatc gtgtttgaag gtcacgacga tctgcggcgc atcctgaccg
      601 attatggttt tatcggtcat ccgtttcgca aggattttcc gttatccggt cacgtcgaga
      661 tgcgttatga cgcggagcaa aaacgtgtgg tgtatcagcc tgtcacgatt gagccacgcg
      721 aaattacccc gcgggtcatc cgtgaggaaa actacgggag cttgcaaaag gcagtttgag
      781 ttatggctga aataaagaat tacacactca acttcgggcc gcagcacccc gcagcgcacg
      841 gtgttttgcg tttggtgctg gaactcgatg gcgaggtgat tcagcgcgct gacccgcata
      901 ttggtttgct gcatcgtgcc acagagaaac ttgccgagag caagacatat ttgcagacgt
      961 tgccttatat ggaccggctg gactacatgt cgatgatgtg taatgagcag gcctattgtc
     1021 ttgccatcga aaaaatgctg ggcttggacg tgcctctgcg cgcccagtac atccgggtca
     1081 tgtttgccga gatcacgcgc ttactgaacc atttgttgtg ggtcggggcg cacgccattg
     1141 actgcggtgc aatgactgtc atgttgtttg cgtttcggga acgtgaagac cttcttgaca
     1201 tgtacgaggc ggttagtggg gcccgcatgc atgcagccta cttgcgccca ggtggtgtct
     1261 atcgtgactt gccagacacg atgccgcgcc atgcacccaa caagattcgc agtgccaagt
     1321 cgaccgaggc gctcaatcgc aaccgtagcg gaagcctgct tgatttcatt gatgacttta
     1381 cgcagcgatt cccgacctat ctgggtgaat accacaccct gctgacggat aaccgcatct
     1441 ggaagcagcg tactgtgggt attggggtgg tgacaccgga acgggcattg aatcttggtt
     1501 tttcgggacc gatgttgcgg ggttcgggtt tcgcctggga tttgcgcaaa tcgcagcctt
     1561 atgaggttta tgacaggctc gactttgaca ttccggttgg caagactggc gatacctatg
     1621 accgctatct ggtgcgcatg gaagagatga agcagtccaa ccgcatcgtc aagcagtgtg
     1681 tcgattggtt gcgggtcaat cctggcccgg tgatcaccag taaccgcaaa gttgcaccgc
     1741 ctgatcgtga atccatgaag tccaacatgg aagatctgat tcaccatttc aagttgttta
     1801 ctgaaggatt ttgcgtccct gagggcgaga cttatgccgc aattgagcat cccaagggtg
     1861 agtttggcat ttacatcatc agtgatggtg ccaacaagcc ttaccgcatg aagattcggc
     1921 ctcctgcttt tgtgcatctg gctgcgctcg atgaaatggg taagggccac atgattgcgg
     1981 atgctgttgc cattattgga accatggata ttgtgtttgg ggaagtggat cgatgatttc
     2041 tgaagaatcc aaaacgcgct tcgcccgcga agttgccaaa taccctgtgg gtcaaaagca
     2101 gtctgctgtg atggcttgtt tggccattgc gcagcaagaa gttggctatg tcagtgaaga
     2161 ggtggaaatc gagattgcgc agtatctgga gatgccagtg atggctgtcc acgaggtgac
     2221 taccttctac aacatgtaca accagcaccc tgttggcaga ttcaagctca atgtttgcac
     2281 taatttgcca tgccagttgc gcgatggtgt caatgccctg aaatatctgg aaaagaagct
     2341 cggcattgca atgggtgaaa cgactccaga tggtctgttt acgctgcaac aaagcgaatg
     2401 tttgggagcc tgcgcggatt caccggtgat tctggtgaat gaccgttcaa tgtgcagctt
     2461 tatgacaacc gaaaagctgg atcagttggt ggatggtttg cgtgcggcgg gagatcaatg
     2521 atgaaggcgc agcaaattct ttcacagttt cgctccgcag gcatagaaac ctgctttcac
     2581 ggtcggcata ttgagccgca aattcttgcc gaactcaatg gttccaactg gcgactgaag
     2641 gactatgaag ggcggggagg ttatcaggca cttcgcaaga ttctcggcaa agacggtagc
     2701 gctggcatga cgcaggacca agtgattgcc acggttaaag aatctgcctt gcgcgggcgt
     2761 ggcggtgcgg ggttcccaac cgggttgaaa tggagtttca tgccgcgtca gtttcccgga
     2821 cacaagtact tggtctgcaa ctctgacgaa ggtgaacctg gtacttgtaa agaccgtgag
     2881 attctggaat tcaatccgca tatcgtgatt gaaggcatgg ccatcgccgc gtacgcaatg
     2941 gggactgcgg taggttacaa ctatatccat ggtgagatct tttcaaccta cgaccgtttt
     3001 gaagaggcat tggaagaggc tcgcgcggca ggtctcctgg gtgacaacat tctgggcagc
     3061 agtttctgct ttcagttgca tgctgcgcat ggattcggtg cctacatctg cggggaagaa
     3121 acggcgctgt tggagtcact ggagggcaag aagggccagc ctcgtttcaa accgccattt
     3181 ccggctagct tcgggcttta tggaaagccc acgacgatca ataatactga gacatttgct
     3241 gctgttccct ggattattcg caatggtggc gcagcgtatg tggcttgtgg aaaacccaac
     3301 aacggtggca ccaagattta ttcagtcagt ggtgatgttg aacgtcccgg caattttgaa
     3361 gtacccatgg gcacaccttt tgccaaggtg ttggagcttg caggtggcgt tcgcgccagt
     3421 cgcaagctca aggcggttat ccctggtggt tcgtcatcgc ctgttctgcc agcagccatc
     3481 atgatggaat gcacgatgga ttacgactcg atcgccaagg cgggctcgat gctgggctct
     3541 ggcgcggtaa tcgtcttgga cgatagccgt tgcatggtca agagcctgca acgtctgagt
     3601 tacttttaca tgcatgagtc ctgcggccaa tgcacgccct gtcgggaagg cactggctgg
     3661 ctttggcgct tggtggaccg catagaaagc gggcagggtc gcgccagcga catggatttg
     3721 cttgacagtg ttgcggggga tattcagggg cgcacgattt gcgcgctggg tgacgcggca
     3781 gccatgccgg tgcgggccat gttgaaacat ttcagacccg aatttgagta ccacgttgag
     3841 cacaagacct gcatggtccc ggcttacgtt tgagcgagtg acaagatgat tgaaattgaa
     3901 ctcgacggca acaaggtgga tattgagcaa ggcagcatga tcatgcatgc cgcagaaaag
     3961 gcaggaacct atatccccca tttctgttac cacaagaagc taagcattgc agccagttgc
     4021 cgcatgtgtc tggtggatgt tgaaaagatg cccaaaccca tgccagcctg cgcgacacct
     4081 gttacgcagg gcatggtggt tcacaccaag accgaaaagg ctgtgaaggc ccagaagtca
     4141 gtgatggagt ttttgctgat caatcaccct ctggattgtc cgatttgtga tcagggtggt
     4201 gaatgtcagt tgcaggacct tgcggtcggc tacggtggaa cttcttcacg ctacgaagag
     4261 gaaaagcgtg tggtttttca taaggatgta gggccactga tttccatgga ggaaatgagc
     4321 cgttgcatcc attgcactcg ctgtgttcgg ttcgggcagg aaattgctgg cgtcatggag
     4381 ttgggcatgt cacatcgcgg tgaacatgca gagatcgaaa cttttgaggg aaggtctatt
     4441 gattccgagt tgtccggcaa catgatcgat atttgtcctg ttggcgcgct caccagcaaa
     4501 ccgtttcgct atagcgcacg gacttgggaa ctggcacgcc gcaagtcggt gagcccgcat
     4561 gactccacag gtgcaaattt gattgttcag gtcaagaaca acaaagtcat gcgtgttgtt
     4621 ccgctggaaa atgaaactgt caatgagtgc tggattgctg accgtgaccg cttctcgtac
     4681 gaggcgttga acggtgatga gcgtctaacc acgcccatgt tgaagcaggg cggcgaatgg
     4741 aaggctgtgg actggaagat tgcgctggaa tatgtggcgc atggcctgca aagcattaag
     4801 agggcgcacg gggccgaagc agttggggca ctggtcagtc cgcacagcac cctggaagag
     4861 ttgtatctcg caggtgctct ggtccgcggt ctcggtagcc agaacattga ttaccgactt
     4921 cgcaatgccg cgtttgagac tgaacccaaa ggtgccaggt atctcgggat gacaatcgct
     4981 gatttgtccc atttgcagcg cgtgctggtg gtgggctcca atcttcgtaa ggaccacccg
     5041 ttgtttgccc agcgcattcg gcaggccgtc aggggcggct gtgtcgtgac ggcactaagt
     5101 gcccgtccga tggattgggc gatgacactg ggtccggtcg acattgttcc ggcatcaggc
     5161 tgggtgcgtg ccttggcaaa tattgcagcc gccattgcgc atgcgaaaaa tgtgcctgcg
     5221 ccacagagtg gtcaggcaac tgatgttgca acggctattg cagattctct tttgagcggt
     5281 gagcgtaaag cagtgctgct gggcaatgct gcggcgcacc atagtcaggc ggatgacttg
     5341 ttggctctgt cactttggat tggtgcgcaa actggcgcat ctgtcgggta ccttactgag
     5401 gctgccaata ctgtgggtgc acaactggtt ggtgcgttgc ctggtccagt tggattcaat
     5461 gcaggccaaa tgttggctgg cgcgcttagg gcaacgcttt tactcaatac agagccgcag
     5521 tttgacgccg cgaacggaga agccgccgtg caggggctta aacaatgcga gatggttgtt
     5581 accttgagcc cattcaaggc aaatctggaa atcagcgatg tgctgctgcc gattgcgcca
     5641 tttactgaaa cacccgggac ttttgtcaat gcggaagggt tgatacagag ttttcatgcc
     5701 gtggtcaagc cgctgggtga agcaagaccg gggtggaaag tgatcagagc actggccaat
     5761 atccttaacc tgccaggatt tgattttgat tccgcgcagg atgtgctgct tcacttgcat
     5821 gccgacaatg ctgggattgt gccagcacgg gtccctgcaa gtcgcctcag caatgaatgt
     5881 ggagtggtat ccgttggtca ggtgtcacag cttgaaccct gtgtggcgac catatatcag
     5941 cttgacggca ttgtgcgccg ctccccttca ctgcagctca ctgcagatgc cagagtggct
     6001 gataccttgg aggtgacagc atgatcgaga gcattttcac atttggtcag ggacttgtcg
     6061 gcgcgggatg gtggacaggc ctcgcgtggc cgacgatatg ggcgctgatt aaggtcgttt
     6121 tggttttgat gctggtgttg actgctgtca catacgccac cttgtgggag cgcaaacttc
     6181 tgggttgggt gcaaattcga cttgggccga atcgagttgg tccgtggggt ctgctgcagc
     6241 cgattgccga tgcactcaag ctgatgacca aggagatcgt gcggccgaca gcgtctgaca
     6301 agtttctctt ttacctgggc ccagtgttaa ccgttgcgcc ggcgctcgct gcttgggcag
     6361 tgattccgtt tggccctgag atcgctgctg caaatctgaa cgccggacta ttgtttttga
     6421 tggcgatcac atcaatggag atctatggtg tcatcgtctc tggttggtcg tcgaactcca
     6481 aatatgcctt tcttggagcc ctgcgcgctt ctgctcagat ggtgagctac gaaattgcca
     6541 tgggattttg cctattgatc gtgttgatgg tgtctgcaag tttgaatctg tcggacatag
     6601 taatgggtca gggaaaaggg tatttcgcgc aaatgggtct atcctttctc tcttggaact
     6661 ggctgccgtt gttgcctgtc tttgtagtgt attttgtgtc gggcttagcg gaaaccaaca
     6721 gaaccccctt tgacgtgatt gagggagagt ctgagattgt cgctgggcat atggtcgaat
     6781 attcaggcat gtcgtgggcc atgttccaga tggctgaata tgcgagtatt tggttaattt
     6841 ccatattggc aaccatcatg tttctgggtg gttggttgtc gcctgttgac agtgtgtttt
     6901 ttaattggat accaggctgg atctggcttg gcattaaaac cttcgggatg atgacgcttt
     6961 ttatatgggt acgggtgact tttccacgtt tccgttatga ccaaatcatg cgcttgggtt
     7021 ggaagatttt tatacccgtg accttggtgt ggctagtggt cattggagct tggatgcaga
     7081 ccccatggaa catttggaag taattcgagc tactatgatt accccaactt caaattctgg
     7141 tgaaaattgc tctgtgagcg tcttttcgct gaaagatttt ctgtcgagtt tctttcttgt
     7201 tgaactgcta aaaggaatgc ggctgaccgg caagtatgca tttcgaagca aaattacgct
     7261 agagtatcca gaggaaaaaa cacctttgtc tccccgcttt cgcggcctgc atgcgttgcg
     7321 tcgttatgac aatggagagg agcgttgtat tgcctgcaaa ctgtgcgagg ctgtatgtcc
     7381 ggcgatggcc atcactattg agtcggaaat tcgtgcggat ggttcgcgtc gtacttcgcg
     7441 ttatgacatc gacttaacta agtgtatttt ttgtggtttt tgcgaagaga gttgtcccgt
     7501 ggattcgatt gtcgaaacaa atattctcga gtatcacggc gaacagcgcg gagacttgta
     7561 tttcaccaaa gaaatgttat tggcagtcgg tgataggttt gagccggaaa ttgcggctaa
     7621 taaaatggct gatgccagat accgctgatt catcgcaagt aggaatttga atcatgagtg
     7681 taacgaccgg tcttttttat tttttttcag tggtgctgtt gtttgcagca tttcgagtga
     7741 tcactgcacg taatcctgtt tatgcggttt tgtatcttgt ccttactttt tttcaggcat
     7801 ccatgatctg gatgttgtta aaggcagagt ttttgtctct ttccctggtt ttggtgtatg
     7861 tgggtgccgt tatggtgctt tttctttttg tcgtgatgat gcttgatgtg aatattgaga
     7921 gcctgcggat tggcttctgg aagcactttc ccctggcagc cgtggtaggc atattcatca
     7981 gtctggaaat gtctgctgtt ctgatggggg ggtttcgagt cagcgctgat cctcatgctg
     8041 caaattcact cgcgagtgtc ggcattgcgg tttccaatac gcgagaactc gggaaactca
     8101 tgtacaccga gtatctgtat ccgctggagg tcgctgcagc tttgcttttg gttgccatga
     8161 tttcggcgat agcgctaacc ttgcgccatc gcaaagaaag caaagcgata aacgctgcca
     8221 atcaggtgca agtcaagtcc cgtgaccgta tgactgtcgt taaagtggtt gccaccaagc
     8281 cagcgttgcc tctgactgag ccggacgcac cttctgcagg gagcaaggta tgacgctgac
     8341 actgggccat tttttgtctt tgggtgcaat tctttttgca ctttccgtga ttggcatttt
     8401 tctaaatcgg agaaatttga ttgttttgat gatggcaatc gaactaatgc tgcttgcggt
     8461 aaatacaaac tttgtcgcgt tttcctatta ccttggggac ttaaacgggc agatatttgt
     8521 tttttttgtt ctaactgtcg cggccgccga ggcagcaatt ggcttggcca ttctggtcct
     8581 gctatttcgc agcaagtcga atataagtgt tgacgaactt gattcgttga aagggtagta
     8641 aaaatgagtc aaaccctttc tgtatccaca ctgcttgcga ttccgctggc ccccctgatc
     8701 ggcgcgatcg ccgccggcgt ttggggtacc aaatttggtg gaaatgttat tggcaggcgt
     8761 ttgtcgcaca cgcttgccat tcttggtgtg ctgatttctt tcgttttgtc ggctggtgtc
     8821 ctgaagagcg tagccatcga tggcgcgcgc ttcaacgaaa ccatttacac ctggatggtg
     8881 cttggtggac tgaaaatgga aatcggattt ctggtggatg gattgagtgc gatgatgatg
     8941 tgtgtcgtga gctttgtctc actgatggtt cacatctata ccgttggcta catggaagag
     9001 gacgaaggtt ataaccggtt ctttgcattc atttcacttt ttaccttttc catgttgatg
     9061 ctggtaatga gcaacaacat gcttcaattg ttttttggct gggaggcggt cggtctggtt
     9121 tcatatttgt tgattggctt ttggttcaac aaaccctccg ccatttttgc caacatgaag
     9181 gccttcttgg tgaatcgagt tggtgatttt ggcttcatct tgggtattgg attgttggtt
     9241 gcctacactg gctcgctcaa ttactccgaa gttttcgcca agtccgtcga aatatccaaa
     9301 ttgaccttgc cgggcactga ttggctgctc gtcaccgtta tttgcatttg tttgttcatc
     9361 ggtgcaatgg gcaagtcggc tcagtttcct ctgcatgtgt ggttgccgga ttcaatggaa
     9421 ggtccaacgc cgatttcggc cttaattcac gctgcaacca tggtgactgc cgggatcttt
     9481 atggtcgcca gaatgtcgcc cttgttcgag ttgagtgatg ctgcccttaa tttcattctc
     9541 gtcattggcg gtattacggc tttgtttatg ggctttttgg gcatcatcca aaatgacatt
     9601 aaacgtgttg tagcttactc aacgttatcg cagcttggtt atatgaccgt cgcgcttggt
     9661 gcctcagcgt actctgtggc tgtctttcac ctgatgacac atgcgttctt caaggccttg
     9721 ctgtttctgg cagcagggtc ggtcatcatg ggcgttcatc acaatcagga catccgatgg
     9781 atgggtggtt tgcgcaagta catgcctgtc acctggatta cttcgctgct tggtt
//
LOCUS       DAOSLL010000096         9812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6680_length_9812_cov_4.391821, whole genome shotgun sequence.
ACCESSION   DAOSLL010000096 DAOSLL010000000
VERSION     DAOSLL010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9812
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6680_length_9812_cov_4.391821"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1645)
                     /locus_tag="PLB25_05940"
     CDS             complement(107..1645)
                     /locus_tag="PLB25_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41154.1"
                     /translation="MKLTFAGVLLSTLLISPLIGCGGSSGSDAPAPLSSQNINLIFVL
                     SPDLTYGASGDVNPITANLTNQGLNRSLKMATYLKTQVLGNQNVTGIYAVAPMTHLQT
                     ANNLPDMASLSAIQQFAMLNQITLPAGAAQPANSFPINVSYAAGMVLPAGVAEPYAGL
                     LPCPACQGLDFSNSGGNNDALVARIVKAKTTGFHVFSAPWETASAMLANINKLYGSSL
                     TMPSGYVGPNLVYAVSITPSGSASLVTYDSHLTPATTAPVLPATLPQTACTAQSQKFS
                     FSITAGQPFSQTLNGTTTTGTTPAVLPAGISTNQTVYMIRHAEAHPTSTFDDGNFVAA
                     GQWRALGLADALRGKINPDMVMSIDPAQVAGIPNNPSMYSYVRTDMTVEPYAIANNLP
                     FYLVASFSLMDIDYTNLPASLSTSQSVINATNYLFMGGNYANKKILLAWEHDHLPPTL
                     NDLLSRYFPKGGAPLFSNTFWPGTDYDTIWTVKLDAQGNLTADNSLCEGIDSAKLPPN
                     APQF"
     gene            complement(1844..3955)
                     /locus_tag="PLB25_05945"
     CDS             complement(1844..3955)
                     /locus_tag="PLB25_05945"
                     /inference="COORDINATES: protein motif:HMM:TIGR03768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03768 family metallophosphoesterase"
                     /protein_id="HOE41155.1"
                     /translation="MKNLDSNKAPDFRVMGDLGRRDFLRYTAGTFAVMSMGSLTAACG
                     GSSDGVTVQTYPIDPSGVKTTVDRMLSFPYTLAAAAPKTGVMPNPADPAKSPNGGPAL
                     TWDKLDQVADYEKLGYGAWTFGAGLPIDPRTDVMADPTGYLAKVKAKQVVPTTKLLNF
                     FAMTDIHITDKEAPNQLIALQKGDPGFSGNNTSIYSPIMLSTTHVLDAAIQTANVLHK
                     TKPFDFFISLGDACNSTLYNELRWYIDVIDGKLIKPSSGAHLGADTIDYQKPFQAVGL
                     DKSIPFYQTMGNHDHFFIGSFPVYESGIAPSFISDTVWSVPYFLEPDPTTFPAMFSVN
                     KMKNDTPRYYGGVIDGATPYGTVVYSGSVASYTTAPKVAADPDRRSLHRTEWIQEFFT
                     TSTAPVGHGFNLVSSNPNFNLVPTSERAGFACYSFLPKSTVPLKVIVLDDTQREDDGS
                     IDIHGHGFLDATRWAWLQKELADGQAANQLMIIAAHIPIAVVNIAGETEWWLGGRKDD
                     PTKLLGDNSTTVQNAVDLAGLVTALQNTPNLLMWIAGHRHLNTVKAFVSPDRINAPEK
                     GFWQVETSSLHDWPQQFRTFEIFINSDYSVSIDVVNVDPAVAEGTPAAKSRKYAIAAQ
                     QIVQNNVTPNSSNYDLQPGTTIPLPSMDPSRKQDSTPDPTIRYGDLSNLGVPYTASYN
                     AQLFKQLTPKMVAVLQSKFPL"
     gene            4217..8032
                     /locus_tag="PLB25_05950"
     CDS             4217..8032
                     /locus_tag="PLB25_05950"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE41156.1"
                     /translation="MSFSKATDSASLTLLGGWQMALVKRRTAEPDYEKGRALLAYLAI
                     EDQLHSREALSELLWPGSPVGRANLRQVLANLRAVLQDDAQVPYILVKRDSLRINPQG
                     RLALDVLAFTAHITADTSLTRLEQLVGLYHGEFMAGFALPDCLEFEDWLHLQRETLHR
                     RALALLEQLSNGYERLGDYPKALQFALRYTALEPWDEVALRRVMRLYALNSQYSAALR
                     QYEACCARLQADLGVLPGEETRQMAERVRQGEWQQRAPRSVPAAVCVSALSGADRRHV
                     TVLYCELTSTCTDDQEDDLALLVAPQTRCAEIIQAFGGHLVQTFGGGLLAYFGYPQAQ
                     EDSARRAVQAALALTRVVPSGQPEAGFELRAGIHTGWIITAGGDAAMPDSVGKTSRRA
                     IALRLCAPPHQVAISAATQRLVGGYFDCNSQGPQVLAGQGPALEVFTVQHQSAARTRL
                     DAARQLSPFAGRVAELARLQSLWQLAAQGKGQAVLLQGEAGIGKSRLLRVFQQQLVRE
                     RHALRELNCFPEFSQSPFHPLIAMFEAILGFRAEDAPEQKRAALSRYFDARYPAARHA
                     DLALLSRLLDVPLAADTVPALPAQQAKERTMGLVLEMLQTLAAQLPTLLIVEDLHWID
                     PSTHELLSRLISQPLRGAVMVIFTARPNFAPSWSQAQCATLPLGPLAQGEVAALVNGL
                     AHQLAPATLGQIVQRADGVALFAEELAHLALLGDDASIPATLQDLLAARVDQLGAAKP
                     VAQMAATLGREFDVDVLQKLWLDSPEALTHGLRALHAADLVVTVSVNTRQFRHALIQQ
                     AAYQSQSKVQRIAVHQRIAEVLVADFPDAGAKRPELVAQHLTAGGDGVTAISYWMRAA
                     QRAALNSANLEAIEHFRSALRLLSELPADLQHQRTERELLLGLCPVLYAVKGYGCEEA
                     ESLSARIAVLNAQLGQNAKLFQTRWTQMVSTIASVSSRGMPQAALQLLAMAQDDPLKK
                     MAAHTLAAIASFWLGDFGQARAHDEHAIALYQPDQRQLQQSQFAAELVVNCEAYQSCA
                     LYFLGFSDQAQKVSRQMLAQARALAHPHNLGQALSFAALLQRWLNQPEAALALAAEAI
                     ALSRQHDFQLWLACGEMTHGWARVKIGQADGIAEISNSVATMRQALGGIPVVFLSSRI
                     EAHVHLRLHAKALDLVAQALADAHNTGDAHYLAELHRLKGVCLLALAPGNAAEAELCF
                     GQALDICRQQNAMPLALRAATSLVSLWQQQGKLEAGRSLLKEMLGAFSEGFDTHDLRD
                     AAQQLQTLG"
     gene            complement(8332..8619)
                     /locus_tag="PLB25_05955"
                     /pseudo
     CDS             complement(8332..8619)
                     /locus_tag="PLB25_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            complement(8553..8774)
                     /locus_tag="PLB25_05960"
     CDS             complement(8553..8774)
                     /locus_tag="PLB25_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41157.1"
                     /translation="MTAFAVSDTLCKRLLSTKLKVGFGFFIEFLGMPAGVGFGSFAAN
                     RSQQRTSPPARLACAWLSVRWKARCCCSF"
     gene            complement(8990..9337)
                     /locus_tag="PLB25_05965"
     CDS             complement(8990..9337)
                     /locus_tag="PLB25_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41158.1"
                     /translation="MTQRTEYIEKMKLQLDKLNKKMSDLEASAQEAKEEALQKYKDDM
                     SKLRQQSKVAVAKLEELKTASVDSWEGMVTDMEKMHDAFTHSFFSLFQTPSPSAPAAS
                     AEVTGSASGHKKV"
     gene            complement(9524..9709)
                     /locus_tag="PLB25_05970"
     CDS             complement(9524..9709)
                     /locus_tag="PLB25_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HOE41159.1"
                     /translation="MNKNQIKGTAKDLAGKVQEEAGKLVGSKEQEAKGIHKQITGKAE
                     RKFGDAKEIVKDAVDRI"
BASE COUNT         2055 a         2720 c         2835 g         2202 t
ORIGIN      
        1 gactgtcggc aaagatcgac aacatgttgg taagtttgat gcgcatagcg cctaagtgcg
       61 gatgctttat aacggtcatt gctttggcaa gcatgtatcc acgcgatcaa aactgtggtg
      121 cgtttggtgg cagcttggca gagtcaatgc cctcacataa cgagttgtcg gccgtcaaat
      181 tgccctgcgc gtcgagtttc actgtccaga tcgtgtcgta gtcggtacct ggccaaaagg
      241 tgttggaaaa caacggggca ccacccttgg ggaagtagcg actgagcaag tcattgaggg
      301 tggggggcaa gtggtcatgc tcccaggcca gcagtatttt cttgttggcg tagttgcctc
      361 ccatgaatag ataattggtg gcattgatga cagattggct agtagaaaga ctggcaggaa
      421 ggttggtgta atcaatgtcc atgaggctga agctggccac cagatagaac ggcaggttgt
      481 tggcgatcgc gtaaggttcg actgtcatgt cagttctgac atacgagtac atgcttgggt
      541 tgtttggtat gccggccact tgcgccggat caatcgacat caccatgtcc gggttgatct
      601 tgccgcgcag tgcatcggcc aagccgagcg cacgccattg accggccgcg acaaagttgc
      661 cgtcatcgaa agtggatgtc ggatgcgcct cggcatggcg aatcatgtaa accgtctggt
      721 tggtgctgat acccgctggc aagacagcag gggtggtgcc agtcgtcgtg gtaccgttga
      781 gcgtttgact gaacggctgg ccagcagtga tactgaaact gaacttctgc gactgcgctg
      841 tgcaagccgt ttggggcagt gtggccggta gcactggagc ggtagttgca ggggtcaggt
      901 ggctgtcgta ggttaccaga ctggcactgc cggagggcgt gatcgacacc gcataaacca
      961 ggttggggcc gacgtagccg gacggcatcg tgaggctgga gccgtacagc ttgttgatat
     1021 tggccagcat ggcactggcc gtctcccaag gggccgagaa gacgtggaaa ccagtggttt
     1081 ttgctttcac gatgcgggcc accagcgcat cgttgttgcc gcccgagttg ctgaaatcga
     1141 gaccctggca ggccggacaa ggtagcagcc ccgcatacgg ctcggccaca ccggcgggca
     1201 gtaccattcc tgcggcataa ctgacattga tgggaaagct gttggcgggt tgcgccgcgc
     1261 ctgcgggcaa cgtgatctgg ttgagcatgg cgaattgctg aatcgcgctt aacgaagcca
     1321 tgtcgggcaa gttattggcg gtttgcaggt gcgtcatcgg cgcaacggca tagatgccgg
     1381 tgacgttctg gttgcccagc acctgcgttt tcagatacgt ggccatcttg agcgaacggt
     1441 ttaacccctg atttgtcagg ttggcggtaa tcggattgac atcaccactg gcgccatagg
     1501 tcaagtcggg gctgagcaca aagatcagat tgatgttttg actgcttaac ggcgcgggcg
     1561 catcgctgcc cgacgagccg ccacaaccga tcaatgggga gatgagtagt gtgctaagta
     1621 acacacctgc gaaagtcaac ttcatttcta tatgcgagaa accccacaac tttgcatggg
     1681 gagggatagc actgcgtgtg agacacgccc cttggttccc gcttcctttc ttatcttgct
     1741 acttttgatt actgggaaag ctcatgcctt caacgtcaaa caccttcatg gctgtacccc
     1801 cacaggcttc ccatcaggca tgctcgttgg cagacacttc ggcctaaagc gggaacttgg
     1861 attgcaatac cgccaccatc ttcggagtca actgtttgaa aagttgggcg ttgtaggacg
     1921 cggtataggg cacgcccaga ttggacaggt cgccgtaccg aatggtcggg tccggggtcg
     1981 aatcctgttt cctggacggg tccatgctgg gcagcgggat tgtcgtaccc ggctgcaaat
     2041 catagttact gctgttcggt gtgacattgt tttgaacaat ctgttgtgcc gcaatcgcat
     2101 actttctcga tttggctgca ggtgtgcctt cggccactgc cggatcaacg ttgaccacgt
     2161 cgatcgacac gctgtagtcg ctgttgataa atatctcaaa ggtgcggaat tgttgtggcc
     2221 aatcatgcag tgacgaggtc tcaacttgcc aaaatccttt ctcaggtgcg ttgatacgat
     2281 caggcgacac aaaagctttg acagtattga gatggcgatg cccggcaatc cacatcagca
     2341 ggttcggcgt gttttgcagt gctgttacca agcctgccag atcgaccgcg ttctgcactg
     2401 tcgttgagtt gtcaccaagc agctttgttg gatcatcttt tctgccgcca agccaccatt
     2461 cggtctcgcc cgcaatattg acaacggcaa tcggaatatg ggccgcgatg atcatgagct
     2521 gattggcagc ctgaccgtct gccagctcct tttgcagcca cgcccaacgc gttgcatcca
     2581 gaaaaccatg gccatgaatg tcaattgagc cgtcatcttc gcgctgcgtg tcgtcaagta
     2641 cgatgacctt gagcggcacc gttgacttcg gcaaaaaact gtaacaggca aaaccagctc
     2701 tttcactggt cggcaccaaa ttgaaattgg ggttggatga aaccaggttg aaaccatgac
     2761 ccactggcgc ggtagaggtc gtgaaaaact cctggatcca ttcggttcta tgcaacgaac
     2821 ggcggtcggg atcagccgca accttgggcg cagtcgtgta gctcgccaca gaccccgagt
     2881 aaaccaccgt gccatagggt gttgcgccgt cgatcacccc accgtagtac ctcggggtgt
     2941 cgttcttcat tttgttcacg ctgaacatcg ccggaaaggt cgttggatca ggctccagaa
     3001 aatagggcac cgaccagacg gtatcgctga tgaaagacgg ggcaatgccg ctttcataaa
     3061 ccgggaacga accaataaag aagtgatcat ggttgcccat ggtctgatag aacggaatcg
     3121 atttatcaag cccaactgcc tggaatggct tctgatagtc aatggtatca gcacccagat
     3181 gggcacccga gcttggctta atcagcttgc catcaatcac atcgatatac caccggagct
     3241 cgttgtacaa ggtgctgttg cacgcgtctc ccagcgagat aaagaaatca aacggctttg
     3301 tcttgtgcaa gacgttggct gtctgaatgg ccgcatccag cacatgcgtg gtggacaaca
     3361 tgatcggcga gtagatcgag gtgttgtttc ccgagaagcc cggatcgcct ttctgcaggg
     3421 cgatcaactg gttgggtgct tccttgtcgg tgatatgaat atccgtcatg gcaaagaaat
     3481 tcagcaattt ggttgtcggg accacctgct tggccttcac ttttgccaaa tagccagtcg
     3541 gatcggccat gacatcggtt cgcggatcga tcggcaaccc cgcgccaaac gtccaggcgc
     3601 cgtagcccag tttttcgtag tcggccactt ggtccagctt gtcccaggtt agagccgggc
     3661 cgccattagg acttttcgcc gggtctgccg gatttggcat gacacctgtt ttcggtgctg
     3721 cagcggccag cgtataggga aatgaaagca tccgatcaac cgtcgttttg acaccggacg
     3781 gatcaatcgg gtaagtttga acggttaccc cgtcgctgct ccctccgcag gcagcagtca
     3841 gcgatcccat ggacatcacc gcaaacgtgc ccgcggtgta tctcaaaaag tcccgccgcc
     3901 ctaggtcgcc catcacgcga aaatcagggg ccttatttga atcaaggttc ttcatggcaa
     3961 ctctttctta gagaaataaa cgattcgatc aatccgtcat gtggacgccg tcacatcagg
     4021 aagtgagtga gctggttcat tgacgattca caatgagggg aaggttattg cgccagcgtc
     4081 tcacgaacgc aatagcggca ataccatagc agccatatca agtatttatt actccgagcg
     4141 gaggtgaagc actgctcttg ccttgcgcta ccatcctccc cattgccatc catcacaacc
     4201 cacaaaaagt ctcgtcgtgt cattcagcaa agccactgac agtgcttccc tgacccttct
     4261 cggtggctgg caaatggcct tggttaagcg ccgcaccgct gaacccgact acgaaaaggg
     4321 ccgcgccctg ctggcctacc tggccattga agaccaactt cattcgcgcg aagcattgag
     4381 cgagctgttg tggccaggct caccagtcgg gcgcgccaac ttgcgccagg tgttggccaa
     4441 tctgcgcgcg gtgctgcaag acgatgccca ggtaccctac attctggtca aacgcgacag
     4501 cctgcgcatc aacccgcagg gccgtttggc gttggatgtg ctggctttca ccgcccacat
     4561 cacggccgac acctctctga cccggctaga gcaattggtc gggctttacc acggtgaatt
     4621 catggctggc ttcgcgcttc ctgattgcct tgaatttgaa gactggttgc atctccagcg
     4681 cgagaccttg caccgccgtg cgctggcctt gctggagcag ttatccaatg gttatgagcg
     4741 gctgggggat taccccaaag cactgcagtt tgccctgcgc tacaccgcgc tggaaccctg
     4801 ggacgaggtg gcgctgcgcc gtgtgatgcg cttgtatgca ttgaacagcc aatacagtgc
     4861 agccctgcgc caatatgaag cctgctgtgc gcgcctgcaa gcggatcttg gcgttttgcc
     4921 tggtgaggag acccgacaaa tggcagagcg ggtgcgtcag ggtgagtggc agcagcgcgc
     4981 acccaggtca gtcccagccg ctgtttgcgt ctctgcgctg tctggtgcag atcgccgaca
     5041 tgtcaccgtg ttgtactgtg aattgaccag tacctgcaca gatgaccagg aagatgattt
     5101 ggcgctgctg gtggcaccgc aaacgcgttg cgcagaaatc attcaggcgt ttggtggaca
     5161 tctggtgcaa acgtttggtg gcgggctgct ggcttatttt ggctacccgc aagcgcaaga
     5221 agactcagcc cggcgtgccg tgcaggcagc gctggcgctg acccgggttg tgccctctgg
     5281 ccagcccgag gccggttttg agctacgggc gggcattcac acgggctgga tcatcactgc
     5341 tggcggtgat gcggccatgc ccgattcggt gggtaaaacc agccgccggg cgattgcctt
     5401 gcgcctgtgc gcgccgcccc atcaagtggc catcagtgct gctacccagc gcctggtcgg
     5461 gggatatttt gactgcaaca gccaaggccc ccaggtattg gctggccagg gaccggcact
     5521 tgaggtgttt actgttcagc accaaagtgc agcccgaacc cggcttgatg cagcccgcca
     5581 attgagcccg tttgccgggc gcgtggccga actggcccgc ttgcaatccc tttggcaact
     5641 agccgcgcaa ggcaaaggcc aggcggtgtt gctgcaaggc gaggccggta ttggaaagtc
     5701 gcgcctgctg cgggttttcc agcagcagtt ggtcagagag cgccatgccc tgcgcgagct
     5761 gaactgtttt ccagaattca gccagtcgcc gtttcacccg ctgattgcca tgtttgaggc
     5821 cattttgggt tttcgcgcgg aggatgcgcc tgagcaaaag cgcgcggctt tgtcgcgtta
     5881 ctttgatgca cgctacccag cggcgcggca tgctgacctg gccctgctgt cacggctgct
     5941 ggatgtgccg ctggcagccg acaccgtgcc agcgctgccc gcgcagcagg ccaaggaacg
     6001 caccatgggc ctggtgctgg agatgctgca gacgctggcg gcacagctac caactttgct
     6061 gattgtggaa gacctgcatt ggattgaccc ctcgacccac gagttattga gtcggctgat
     6121 cagccaaccg ttgcgcggcg cagtgatggt catttttacc gcccggccta actttgcgcc
     6181 gtcttggtcg caggcgcaat gcgccacgct gccattgggt ccgctggcac aaggcgaagt
     6241 ggcggcattg gtaaacggct tggcgcacca actggcaccg gccacgctcg ggcaaattgt
     6301 gcagcgcgct gacggtgtgg ccctttttgc cgaagagctg gcgcacttgg cactgcttgg
     6361 cgacgacgcc agcattccag ccacgttgca agatttgctg gccgcgcggg tcgaccagct
     6421 cggtgcggcc aagcctgtgg cccaaatggc ggccaccttg gggcgcgaat ttgacgtgga
     6481 tgtgttgcaa aagctgtggt tggactcgcc cgaagcactg acccatggct tgcgtgccct
     6541 gcacgctgcc gacttggtgg tcacagtctc ggtcaacacc cggcaattca gacacgcact
     6601 gatccagcag gcagcctacc aatcacagtc aaaagtgcag cggatcgctg tgcatcagcg
     6661 tattgcggag gtcttggtgg cggattttcc cgacgctggc gcgaaacgcc cggaactggt
     6721 ggcgcaacat ttaactgccg ggggtgacgg cgtgacagcc attagctact ggatgcgcgc
     6781 agcgcagcgg gccgcactta actcggccaa cctcgaagcc atcgagcatt tcagatcggc
     6841 cttgcgcttg ttgtcagagt taccagccga cttgcagcac caacgcaccg aacgcgagtt
     6901 attactgggc ttatgccccg tcttatatgc agtcaagggc tatggttgcg aggaagcaga
     6961 gagcctgagt gcccggattg cggttttaaa tgcgcaactg ggccagaatg ccaagctgtt
     7021 tcagacccgc tggacgcaga tggtcagcac tatcgccagc gtcagctcgc gcggtatgcc
     7081 gcaagcggca ctgcaactgc tggcgatggc acaggacgat cccttgaaga aaatggcggc
     7141 ccatacactg gcggcaattg cttcattctg gctcggcgat tttggacaag cgcgcgcgca
     7201 cgatgaacac gccattgcgc tgtaccagcc cgatcagcga cagttgcagc aaagccagtt
     7261 tgccgcagaa ctagtggtga actgcgaggc ctaccagtcg tgtgcgctgt actttctggg
     7321 tttctcagat caagcccaga aagtttcacg gcagatgctg gcgcaggcac gcgccctggc
     7381 gcacccgcac aacctggggc aggcactgag ttttgccgcc ctgttgcagc gctggctgaa
     7441 ccagcctgag gccgccctgg ccttggcggc agaggccatt gccctgtcac gtcaacatga
     7501 ctttcagctc tggctggctt gtggcgagat gacgcatggc tgggcgcggg tcaagattgg
     7561 acaagctgac ggcatcgctg aaatcagcaa cagtgtggcg accatgcgtc aggcgctggg
     7621 tggcataccg gttgtttttt tatcgtcccg gattgaagcc cacgttcatc tgagactcca
     7681 cgccaaggcg cttgacctgg tggcacaggc actggcagat gcgcataaca caggggatgc
     7741 acattacctg gccgagttgc accggctcaa aggcgtctgt ttgctggcac ttgcacctgg
     7801 caatgcggca gaagccgaac tgtgtttcgg ccaggcgctg gacatttgcc gtcaacaaaa
     7861 cgcaatgcca ctggcgctgc gcgccgccac cagcttggtg agcttatggc agcaacaagg
     7921 caaacttgag gctgggcggt cgctacttaa ggagatgctt ggcgctttca gtgagggttt
     7981 cgacacccac gacctgagag atgcggccca acagttgcag actttgggct aattctggat
     8041 ctgactcgat tcttaaaatc tctgtgatcg attcctgtga ttgcgacagg aaccaaagaa
     8101 tttatgactg gcgacagctc cgtcgtcatt ccgttgagcg tcaattggtt gactcaatga
     8161 ccggtatcgg gctggcatag cgccagtctg aatgtcggca gagggcacca aggcgaccga
     8221 cacgaacggc ggctgatcgg tcacggaaat catgaagctg acttacctct aagcggccac
     8281 tggtggcggt ctgctccccc gcccgaccac ccatccaacc caaccaactc gctcatgccg
     8341 cagtggctca atcgggtcca tgcgggccgc gcgccgcgct ggaaagtacc caaacacaac
     8401 accattcccg gccgaaaaca ccatcgccag caggttgatc gccgcatcaa actcataggg
     8461 caccgccatg tacaaagaca agccatacga agccccggtg gcaatcagca ccccgaccac
     8521 accgcccagc gccgacagca ccaccgcttc tatcaaaaac tgcagcagca cctcgccttc
     8581 cagcgcaccg acagccaggc gcaggccaat ctcgcgggtg gacttgtccg ctgttgcgaa
     8641 cggttggctg caaaagagcc aaagccgaca cccgcaggca taccaagaaa ctcaatgaaa
     8701 aatccgaagc cgaccttcag tttcgtcgaa agcagacgtt tgcataaggt gtcggaaact
     8761 gcaaaagcag tcattcagcg gcgcaattcc acctttgctg ttgaccgtcg gcactggcgc
     8821 ggtagctgtc agtcgaatca aagtcgggag cacccggttt gagccaattt gcagtcggcc
     8881 gccaatggct ggtctggagc atctgaaatt cacatcaaca agatgccaga tgtctgaccg
     8941 ccatcggcac cagtaacgtg cacaaagcga ccaacaagac cgagttggtt cagaccttct
     9001 tgtgaccgct tgcactgcct gttacttcgg cagaggccgc aggggcgctg ggactgggag
     9061 tttgaaaaag cgagaaaaag gaatgcgtga aggcatcgtg cattttttcc atgtcggtga
     9121 ccatgccttc ccaggagtct acgctggccg ttttgagctc ttctagtttg gcaacggcaa
     9181 ctttggattg ttgacgcagt ttgctcatgt catccttgta tttttgcagg gcttcctctt
     9241 ttgcttcctg tgcggaggct tcaaggtcgc tcattttttt attcaactta tcgagttgaa
     9301 gtttcatctt ttcgatgtat tcggtacgtt gtgtcatggt agtaattcct tgctgttttt
     9361 aagccaatgg ctggcttgag gacgatcaga tagcggtttg catcaacttg attgccttct
     9421 atcgggccag cccaccgggg ccagcaaaat ttataggaag ttggtggtca ccaaaggcac
     9481 cactgcgcag tggtccggtg ccgctgaaac cctcaagaag cttttagatg cggtcgacgg
     9541 catccttgac gatttccttg gcatctccaa atttcctttc tgctttgccc gtaatctgtt
     9601 tgtgaatgcc cttggcttct tgttccttgc ttccaaccaa tttcccggct tcctcttgaa
     9661 ctttgccagc gaggtcttta gcggtacctt tgatttgatt tttgttcatg atggttttcc
     9721 ttagaaagtg aaagaattgc tttgcgtgcg agccagttaa cagctggaca gtgccaagtt
     9781 ctccccgtta ttcaggcggt ttcttggcca gg
//
LOCUS       DAOSLL010000097         9774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6712_length_9774_cov_4.243544, whole genome shotgun sequence.
ACCESSION   DAOSLL010000097 DAOSLL010000000
VERSION     DAOSLL010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9774
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6712_length_9774_cov_4.243544"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="PLB25_05975"
     CDS             complement(<1..322)
                     /locus_tag="PLB25_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41160.1"
                     /translation="MRVIFHEDFHQSFYSDDEFDNGAAEGRLVNLMATLRDDGRYELA
                     VPAPATRADLLRAHTEAHIATIEAKPRLFAMACLAAGAAILASDLALQGEPSFACLRP
                     PGHHA"
     gene            451..834
                     /locus_tag="PLB25_05980"
     CDS             451..834
                     /locus_tag="PLB25_05980"
                     /inference="COORDINATES: protein motif:HMM:NF033749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOE41161.1"
                     /translation="MSIEWNDSFKIGDTEVDDQHKYLFELTNEFIAADSLGSMRSLMI
                     LLYKHTREHFEREEVLMRRVNFPGLALHQEHHNTLLRRLSELSMDVGKGYMNKPAIVA
                     LMTDWATKHIPQEDAQVVAFMAKQP"
     gene            857..1714
                     /locus_tag="PLB25_05985"
     CDS             857..1714
                     /locus_tag="PLB25_05985"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOE41162.1"
                     /translation="MMRWIDTHCHLDAQEFGPLALAQLALAATDNVVHWVLPAVQVGN
                     FEAVRRLAHAGGGSYCLGIHPMYVAGADDAALGQLADALKRQHDDPRLVAVGEIGLDF
                     FVPALCESPLRERQQHFYREQLKLARLHELPVVLHSRRAVDQVLRGLREVAGRAERWH
                     GIAHAFNGSFSQAQAFTGLGLKLGFGGAFTYERALQLRRLATELPPDVVVLETDAPDM
                     PPRWLYKTMAQRAAGAVQSVNSPAELPRIGAELAALRGIRVEDLAATTTRNAQAALPR
                     LAALLTMPA"
     gene            complement(1780..3825)
                     /locus_tag="PLB25_05990"
     CDS             complement(1780..3825)
                     /locus_tag="PLB25_05990"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE41163.1"
                     /translation="MFGQLERSLTKTLYRQLVSGVALVLALVMSLFSWEMIRQQEATE
                     VSQFTRQALALASSMAKSSDVWVASRDFSGLQEIVLGLAGYPDLLHAMVLDNRGQVLA
                     HSDPGRRGKYLTDLPAPIEPRILWLDQQHIDVVTPIMLADRHVGWARVGLGRAIFNAQ
                     FASTQRDAILYSLVTIVLGALFAALAVRYLTRRLRALQQVADAVHAGQLDRRVLVTGQ
                     DEAAQLASHFNAMLDRLAAREEALKESETLLNATQQLGKIGGWQWDIEKQSMLWTPQT
                     YRIHDMSPELASTTLGSDRIDESLACYLPQDRDTITKAFWRCVEHGEPYDIEVQFITV
                     KKRLLWIRTMAKAIRHEGQIVKVLGFIMDITERRQLEQLNRAAALYSRTLLEASLDPL
                     VTINAQGKITDANRATELATGVSRDKLIATDFADYFTSPDKARAGYLKAFSQGFVINY
                     PLAIQHLSGRITEVIYNASVYRAEDGSIQGVFAAARDITELKKMEEEVRQLAFFDPLT
                     MLPNRRMFADRLSQAMVASQRNGSFGALMYLDLDNFKPVNDHFGHAAGDLLLVEVAVR
                     LTGCVRAVDTVGRTGGDEFVVILGALSAILSEATAEASQIAEKIRAVLAQPYFLVLPQ
                     QGGKAEQVEHVCTASIGVTMFMGFEASQDGLMQRGDTAMFLAKEAGGNRVLFHQPRV"
     gene            complement(3825..4712)
                     /locus_tag="PLB25_05995"
     CDS             complement(3825..4712)
                     /locus_tag="PLB25_05995"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOE41164.1"
                     /translation="MSLLVGLHLQACDQQPAPVALRYSEAPMVRSEPVYRLAIHPLHN
                     PKKLSAAYQPLVDHLNAQLQGARIELEASRDYQAYEAKFRAREPDFLLPNPWQTLQAI
                     KVGYHVMAMAGDAEDFKGIFIVRKDSGIKRPADLKGKTVSYPSHTALAAAIMPQYYLH
                     ANGIDIRHDIQNAYVGSQESSIMNVYLGQAAAGATWPPPWRLFQKDHPAEAAQLKVIW
                     QTPSLINNSVMVRDDVPPVVRDTVAKALLALSQTAGGTTILGAMEITRFHAASDASYD
                     VVVNYIRRFEHDVRPVESP"
     gene            4909..5358
                     /locus_tag="PLB25_06000"
     CDS             4909..5358
                     /locus_tag="PLB25_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016454716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HOE41165.1"
                     /translation="MPHDHPALDTDQQAIDHILKNCRSVAVVGLSPKAHRDSYRVAHY
                     LQQRGWRVIAINPVAAASGDTILGERVYASLSEAAQHEKIDLVDVFRNSEDVPPVVDE
                     AIAIGAPALWLQLGVRHDVALQKARTAGLVAVQNLCLKVEHARRGIA"
     gene            5392..6150
                     /locus_tag="PLB25_06005"
     CDS             5392..6150
                     /locus_tag="PLB25_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HOE41166.1"
                     /translation="MLKKRTPVLPEVNFSDDGRIRHLHLGTQWIQGSMYLDNPISLVH
                     EYIQRMMAWLLFVEPDTVKDRCAMQLGLGAASLTKFCAKELRMKTTAVELNPQVLAAC
                     HGWFKLPANSPGMQVVLADAAEEILNPEWWGTVDALQVDLYDHEAAAPVQDSLPFYNH
                     CRRLLTPEGCMTVNLFGRASSFTRSVEKISAAFGKDAVWAFKPTREGNTVVLAQHTAS
                     RPRREDLMARANLIEARWGLPADKWVRVFKPLLP"
     gene            6147..7931
                     /locus_tag="PLB25_06010"
     CDS             6147..7931
                     /locus_tag="PLB25_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HOE41167.1"
                     /translation="MNQTAHAKLSEPAASGPLDWRSLVQWLQADGVIAAKEAQRIHTR
                     CAQAESVQHPLVRLSSVAVHRASDDKPMDIEQLTEWLAGRARLAYLRIDPLKVDVSKV
                     ADTMSPAYAERHKILPVQVTTHEVVVATAEPFLTDWVAEVERQARRSVRRVVASPQDI
                     HRFTVEFFALAKSVRAANKAGSNTAASFEQLVELGKSNKQLDANDQSVVQVVDWLWQY
                     AFDQRASDIHLEPRREQGVIRFRIDGVLHPVYQMPMGVLVAMTARIKLLGRMDVVERR
                     RPQDGRIKTRNPGGSEIEMRISTLPTAFGEKMVMRIFDPETTVKDLDALGFSAHDAQR
                     WESLVKRPNGIILVTGPTGSGKTTTLYSTLKRVATEAVNVNTVEDPIEMIEPSFNQTQ
                     VQPQLDFGFPEGLRALMRQDPDIIMVGEIRDQATAEMAVQAALTGHLVFSTLHTNDAP
                     SAITRLMELGVPSYLINATLLGVLAQRLVRTLCPICKLPDPAARREMLAEVVKPWQVN
                     GTYKPFKAVGCVDCRLTGFMGRMGLYELLVVTEAFKAHVTREPDAGALKRQAVTDGMR
                     PLRLAGALRVAEGLTLLDEVLSSTPALG"
     gene            8212..8712
                     /locus_tag="PLB25_06015"
     CDS             8212..8712
                     /locus_tag="PLB25_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOE41168.1"
                     /translation="MRIRNQKDSFSGLLFMLVGLAFAWGASQYRIGISASMGPGYFPL
                     MLGLMLALLGVAIVLRALAWRAPPDAADPGKVGAWAVKPLFFIIAANLLFGLLLGGLP
                     RFGLPAMGLVLAIYGLTVVAGMAGERFSLKESLLLATVLALGSYLAFVLLLKLQFVVW
                     PAFLSG"
     gene            8721..>9774
                     /locus_tag="PLB25_06020"
     CDS             8721..>9774
                     /locus_tag="PLB25_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOE41169.1"
                     /translation="MDLISNLSMGFGVAATPINLLYAFVGCLLGTLIGVLPGIGPVAT
                     IAMLLPATYALPPVSALIMLAGIYYGAQYGGSTTAILVNLPGEASSVVTVIDGHQMAR
                     KGRAGPALAAAGLGSFFAGCVGTLTLAAFATPLTEVAFKFGAAEYFALMVLGLVGAVV
                     LASGSLLKAVAMIVLGLLLGLVGTDVNSGVVRFAFDIPELTDGIGFIVIAMGIFGYGE
                     IIANLSIKAAQREVFAHKVHGLMPTREDFRRMWPAVLRGTAIGSMLGILPGGGALLAA
                     FAAYSVEKKTRLQAGELPFGQGNIRGVAAPESANNAGAQTSFIPLLTLGIPPNPVMAL
                     MVGAMTIHNIQPGPQVM"
BASE COUNT         1867 a         2822 c         3056 g         2029 t
ORIGIN      
        1 tggcgtggtg accgggcggg cgcaggcagg caaagctggg ttccccttgc aaagccaggt
       61 cagacgccag aatggcagcg cctgcggcca ggcaagccat ggcaaacagg cgtggtttgg
      121 cttcaatggt cgcgatatgg gcttcggtgt gggcgcgcaa caagtcagcc cgggtggccg
      181 gggcaggcac cgcaagctcg tagcggccgt catcgcgcag ggtggccatc aggttgacca
      241 gccgaccctc ggccgccccg ttatcaaatt catcatcgga gtagaaagac tggtgaaaat
      301 cttcgtgaaa aatgacacgc attgggtagt ttcttttaac ttcaggtcaa atcaggcaat
      361 ggttagagta ccggcctgcc tgctacagat gccgaatggg ccaggctgcc ggtgtatagt
      421 cgaatccaat tattgttgga gtaccagttt atgagtatcg aatggaatga cagtttcaaa
      481 attggtgaca ccgaggtgga tgatcagcac aagtatttgt ttgaactgac caatgaattc
      541 atagcggctg acagcctggg cagcatgcgc tccttgatga ttttgctata caagcacacg
      601 cgcgagcatt tcgagcgaga agaagtcctg atgcgccggg tcaactttcc gggtcttgcc
      661 cttcatcagg agcaccacaa caccttgttg cgccgcctta gcgaactgag catggacgtg
      721 ggcaagggct acatgaacaa gcccgccatc gtcgccctga tgactgattg ggccaccaag
      781 cacattccgc aagaggacgc gcaagtggtg gccttcatgg ccaagcagcc ctgagccatg
      841 ctggcagcgc gccagtgtga tgcgctggat tgacacccat tgccacctgg atgcacaaga
      901 gtttgggcct ctagcccttg cgcagcttgc gctggcagct actgataatg tagtgcactg
      961 ggtcttgcct gcagttcagg tcggcaactt cgaggcagtg cgacggctgg cccatgcagg
     1021 cggtggcagc tactgcctgg gcattcaccc gatgtatgtg gcaggtgccg atgacgcggc
     1081 actcggccaa ttggccgatg cgctcaagcg ccagcacgac gatccgcgcc tggttgcagt
     1141 gggcgaaatc gggctggact tctttgtacc cgcgctgtgc gaaagccccc tgcgtgaacg
     1201 ccagcagcac ttttaccgtg aacagctcaa attggcgcgt ctgcatgaac tgccggtggt
     1261 gctgcattcg cgacgcgcag tggaccaggt actgcgcggc ctgcgcgaag tggcggggcg
     1321 ggccgaacgc tggcatggca ttgcgcatgc cttcaatggc agcttcagtc aggcacaggc
     1381 tttcaccggt ttgggtctca agcttggctt tggtggtgcc ttcacttatg aacgggcgct
     1441 gcagttgcgc cgcctggcga ctgaactgcc gcccgatgtg gtggtgctgg aaaccgacgc
     1501 acccgacatg ccaccacgct ggctttacaa aaccatggcg cagcgtgctg ctggcgcggt
     1561 tcaatctgtc aattcgcctg ccgagttgcc gcgcataggc gctgaactgg cggcactcag
     1621 aggcatccgg gtggaagacc tggccgctac caccacccgc aacgcgcaag ccgcgttgcc
     1681 acgactggct gcgctgctga ccatgcctgc ctgagacgtt ccggcccaaa agacgaagga
     1741 ctggtccagg tcagcgtgac ctgccctgtc gggccactgt cagacgcgcg gctggtggaa
     1801 caaaacccgg ttgccgccag cttctttggc caggaacatg gcggtatcac ccctttgcat
     1861 gaggccgtcc tggctggcct caaagcccat gaacatggtg acaccaatgc tggccgtgca
     1921 gacgtgttcg acctgttcgg ctttgccgcc ctgctgcggc agcaccaaaa aatagggttg
     1981 tgcaagcact gcccgaattt tttcggcgat ctggctggcc tctgccgtgg cttcggacag
     2041 aatggcgctc agggcaccga ggatcaccac aaactcgtcg ccaccggtac ggcctacggt
     2101 atcgacggca cgcacacagc ctgtcaatcg caccgccact tcgaccaata acaggtcgcc
     2161 tgcggcatga ccgaaatggt cattgaccgg tttgaaattg tccagatcaa ggtacatcaa
     2221 ggcaccaaag ctgccattgc gctgactcgc caccattgcc tgactcaagc ggtccgcgaa
     2281 catacgccgg ttgggcagca tggtgagtgg gtcaaagaag gccagttggc ggacttcttc
     2341 ctccattttt ttcagctcgg tgatgtcgcg ggcggcggca aagacacctt gaatgctgcc
     2401 atcttcggca cggtaaacgc tggcgttgta gatcacctcg gtgattcttc ctgacagatg
     2461 ttgaatggcc agcgggtagt tgatgacaaa cccttgcgag aacgccttca ggtagccagc
     2521 gcgtgccttg tcggggcttg tgaaatagtc ggcaaaatcg gttgcgatca atttgtcgcg
     2581 gctgaccccg gtggccagtt cggtggcccg gttggcatcg gtgattttgc cttgggcgtt
     2641 gatggtcacc agcgggtcaa ggctcgcctc aagcagcgtg cggctgtaga gtgcggcggc
     2701 ccggttaagc tgctccaatt gccgacgttc ggtgatgtcc atgatgaagc ccagtacctt
     2761 cacgatctgg ccctcatggc gaatcgcctt tgccatggtg cgaatccaca gcaaccgttt
     2821 tttgacggtg atgaactgga cttcgatgtc atagggttca ccgtgctcaa cgcagcgcca
     2881 gaacgccttc gtgatggtgt cacggtcttg cggcaaataa caggcaagac tttcgtcaat
     2941 gcggtcagaa cccaaagtgg tgctggcaag ctcaggtgac atgtcgtgaa ttcggtaggt
     3001 ctgcggtgtc cagagcatgg attgtttttc gatatcccac tgccagcccc caatcttgcc
     3061 aagctgctgc gtggcattta acaaagtttc actttctttc agggcttctt cgcgtgccgc
     3121 cagccggtcc agcatggcat tgaagtggct ggcaagctgg gccgcctcgt cttggcccgt
     3181 cacaagcacg cgccggtcaa gctgtccggc atgtaccgca tcggcaactt gctgcaaggc
     3241 acgcagccga cgggtcaggt agcgaacggc caatgcggca aacagcgcgc ccagcacgat
     3301 ggtaaccagg ctgtacagga tggcgtcacg ctgtgtgctg gcaaactggg cattgaatat
     3361 tgcccgcccc aacccgacgc gagcccagcc aacatggcga tctgccagca tgatgggtgt
     3421 gacgacatca atatgctgct ggtccagcca cagaatgcgg ggttcaatcg gcgcaggcaa
     3481 atcggtcagg tacttaccac gccgaccggg gtcgctatgg gccagcactt ggccccggtt
     3541 gtcgagcacc atggcgtgaa gcaaatcagg gtaaccagca agacccagca caatttcctg
     3601 caagccactg aagtcacgcg aggccaccca gacatccgag cttttcgcca tgctgctggc
     3661 cagtgccagc gcctgacgag tgaattgact cacttcggtg gcttcttgct ggcgaatcat
     3721 ttcccacgaa aacagcgaca tgaccagggc caataccagg gcgacaccac tgaccaattg
     3781 ccggtaaagg gttttcgtca gcgagcgttc aagttggcca aacatcaggg actctctacc
     3841 gggcgcacat cgtgctcgaa gcgccggatg taattgacca ccacgtcgta actggcatcg
     3901 ctggcagcat ggaagcgggt gatttccatc gccccgagga tcgtcgtgcc gcctgctgtc
     3961 tgtgagagtg caagcagtgc ttttgcaaca gtgtcacgca cgaccggcgg tacatcgtcg
     4021 cgcaccatga ccgagttgtt gatcagcgaa ggagtttgcc agatcacttt gagttgcgcg
     4081 gcttctgccg gatgatcctt ttgaaacagg cgccacggtg gtggccaggt ggcacctgct
     4141 gccgcctgac ccagatagac gttcatgatg gaagactctt gcgaacccac ataggcgttt
     4201 tgaatgtcat gccggatatc gatgccgttc gcgtgcaggt aatactgggg catgatggca
     4261 gccgccagcg ccgtatgcga ggggtaactg acagttttcc ccttgaggtc cgcgggccgc
     4321 ttgatgccgc tgtcttttct gacgatgaaa atacccttga aatcttcggc atctccggcc
     4381 atcgccatca cgtggtagcc gaccttgatg gcttgcaggg tttgccacgg gttgggcaac
     4441 aggaaatctg gctcgcgggc gcgaaacttg gcctcataag cctggtaatc acgcgaagct
     4501 tcaagctcga tgcgagcgcc ttgcaactgt gcgttgaggt gatccaccag cggctggtaa
     4561 gccgcgctga gctttttcgg gttatgcagc gggtggatcg ccagacggta aacaggctca
     4621 ctgcggacca tgggagcttc actgtagcgt agcgcgaccg gtgccggctg ctgatcgcag
     4681 gcctgcaggt gcaggcccac aagcaagctc acagcgactc tgaagctgac cttcaggcgg
     4741 ctgattacgg cgcggcagtc agagcgttgg gcttccattg ccaatgaatg tatcactttg
     4801 gcgcgacatt gaagtggtct gacagagggt caaaaggcca cttgattggt aaaaaacgtt
     4861 gcactgtaag cctgtaatat ccgggcaacc gcaacccgaa agaaaaacat gccccatgac
     4921 catccggcct tagacaccga ccagcaagcc attgatcaca tcctgaaaaa ttgccgcagt
     4981 gtggcggtgg tgggcttgtc gcccaaggcg caccgggaca gttaccgggt ggcgcactac
     5041 ctgcagcagc gcggctggcg cgtcattgcc atcaacccgg tggcagcagc cagcggtgac
     5101 acgattctgg gtgagcgggt ttatgccagc ctcagtgagg ctgcacaaca tgaaaaaatt
     5161 gacctggtcg atgtatttcg caacagtgaa gacgtgccac cggtggtgga cgaggcgatt
     5221 gcaattggcg caccggcgct gtggctgcag cttggggtac ggcacgatgt agccttgcaa
     5281 aaggcccgca cggcaggcct ggtggcagtg caaaacctct gcctgaaagt ggaacatgcg
     5341 cgccggggca tcgcctgatc tggtgccgca gcgtttgggg ataatccctg catcttgaaa
     5401 aaacgcacac ccgttctccc tgaagtcaat ttctctgacg atggccgcat tcggcacctg
     5461 catctgggca cccaatggat tcagggctcg atgtatctgg acaacccgat cagcctggtg
     5521 catgaataca tacagcgcat gatggcctgg ctgctgtttg tcgaaccaga cacggtcaaa
     5581 gaccgctgtg ccatgcaact cgggctgggt gccgcttcac tgaccaagtt ttgcgccaag
     5641 gagttgcgca tgaaaaccac cgccgtcgaa ctcaacccgc aagtgctggc ggcctgccat
     5701 ggctggttca agctgcccgc caatagcccc ggcatgcagg tggtgctggc agatgccgca
     5761 gaggaaattc tcaaccccga atggtggggt acggtggatg ctttgcaggt ggatttgtat
     5821 gaccatgaag cggctgcgcc ggtgcaggac agtctgcctt tttacaacca ctgccgccgc
     5881 ttgctgacgc ctgagggctg catgaccgtc aatctgtttg gccgggcatc gagttttacc
     5941 cgcagtgtcg aaaaaatatc ggcagccttt ggcaaggatg ccgtttgggc cttcaaaccc
     6001 acccgtgaag gcaacaccgt ggtgctggcg cagcacacag ccagccgtcc ccggcgcgag
     6061 gatttgatgg cccgtgccaa cctcattgag gcgcgctggg gtctgccggc cgacaaatgg
     6121 gtgcgtgtct ttaaacccct gttgccatga accagactgc tcacgccaaa ctgtcagaac
     6181 cggctgctag cggaccgctg gactggcgca gcctggtgca atggctgcag gccgatggtg
     6241 tcattgccgc caaggaggcg cagcgcattc acacccgctg cgcccaggcc gagagcgtgc
     6301 agcatccgct ggtgcgactt tccagtgttg ccgttcaccg ggccagcgac gacaagccga
     6361 tggacatcga acagctcacc gagtggctgg ccgggcgcgc cagactggcc tatttgcgca
     6421 ttgacccgct caaggttgat gtcagcaagg tggccgatac catgagcccg gcgtatgccg
     6481 agcggcacaa gattttgcca gtgcaggtaa cgacacatga ggtggtggtg gccaccgccg
     6541 agccgttttt gactgactgg gtggcagaag tggagcgcca ggcgcgtcgc agcgtgcgtc
     6601 gggtggtggc cagcccgcag gacattcacc gttttacggt tgagtttttt gcgctggcca
     6661 aatcggtgcg ggcagccaac aaggcgggta gcaataccgc tgccagtttc gagcaattgg
     6721 ttgagctggg caagagcaac aagcagctcg atgccaacga ccaaagcgtg gtgcaggtgg
     6781 tggactggct gtggcaatac gcatttgacc agcgcgccag cgacattcac ctggagccac
     6841 ggcgcgagca gggggtgatc cgctttcgca ttgacggtgt attgcacccg gtttaccaga
     6901 tgccgatggg cgtgctggtc gccatgaccg cccgcatcaa gctgctgggc cgcatggacg
     6961 tggtggagcg acggcgcccg caggatggtc gcatcaagac gcgcaacccc ggcggcagtg
     7021 agatcgagat gcgcatttcc acgctgccga cggcttttgg cgaaaagatg gtgatgcgca
     7081 tttttgaccc ggagaccacc gtcaaggacc ttgatgcgct gggtttttct gcgcatgatg
     7141 cgcagcgctg ggagtccctg gtcaagcgcc ccaacggcat cattctggtg accggtccaa
     7201 ccggctcggg caaaaccacc accctgtatt cgaccctgaa gcgggttgcc accgaggcag
     7261 tgaatgtcaa cacggtagaa gacccgatcg agatgattga gccatcgttt aaccagacgc
     7321 aggtgcagcc acagcttgat tttggtttcc ccgaaggatt gcgtgccctg atgcggcaag
     7381 acccggacat catcatggtg ggtgaaattc gcgaccaggc caccgccgaa atggcggtgc
     7441 aggccgcgct gaccgggcac ctggtgttct ccaccctgca caccaacgac gcaccatcgg
     7501 ccatcacccg cctgatggag ctgggcgtgc cgtcatacct gatcaacgcc accttgcttg
     7561 gcgtgctggc gcagcggctg gtgcgcacct tgtgcccgat ttgcaagctg cccgacccgg
     7621 ctgccaggcg cgagatgctg gccgaggtgg tcaaaccctg gcaggtcaat ggcacttaca
     7681 agccattcaa ggcggtcggt tgtgttgatt gccgcctgac cggcttcatg gggcgcatgg
     7741 gcttgtacga gttgctggtg gtgactgaag cgttcaaggc gcacgtcacc cgtgagcccg
     7801 atgctggcgc gctgaaacgc caggcggtga cagacggcat gcgcccactg cgactggctg
     7861 gtgcgctgcg cgtggccgag ggcctgacgc tgctcgatga agtgctgtca tcgacaccag
     7921 cgctgggttg attgcggcag actgaaacca cgcacgccat gccaagccga ccgattctgc
     7981 gcttgacggt ggcctgctcg cgccagtccg gccaagcatc cgcagcgtca ggtcggttgc
     8041 atttgctggt ggcctttcgg cacagcagta ccagccctga cagcgctgcg cctgtttcca
     8101 tcggtccgca cgccggatga tcttgcgcag acccggcaaa tccacctgca gcattgcctg
     8161 gctgcggtgt ttgcggtcat ttgtgcgcgg cccgtcttcg ccaccgatgc catgaggatc
     8221 aggaaccaga aggattcttt ttccggcctg ctgttcatgc tggtcggtct ggcgtttgcc
     8281 tggggtgcca gccaataccg cattggcatc agtgccagca tggggccggg ctattttcct
     8341 ttgatgcttg ggctgatgtt ggcactgctg ggggtggcga ttgtgctacg cgcgctggcc
     8401 tggcgtgcgc cgccggatgc agccgacccc ggcaaggtgg gcgcctgggc cgtcaagccg
     8461 ctgtttttca tcatcgctgc caatttgctg ttcggtttgc tgctgggggg tttgccccgt
     8521 tttggcctgc ccgcgatggg gctggtgctg gccatttacg ggctgacggt ggtggcaggc
     8581 atggcgggcg agcgctttag cctgaaagaa agcctgcttt tggcaaccgt gctggcgctt
     8641 ggcagttacc tggcttttgt gctgctgctg aagctgcagt ttgtcgtctg gccagccttt
     8701 ttgtcgggtt aagcgggcgc atggacctga tcagcaacct gtcgatgggc tttggcgtgg
     8761 ctgccacgcc gatcaacctg ctgtacgcct ttgtcggctg cctgctgggc acgctgattg
     8821 gtgtgttgcc cggcattggt ccggtagcca ccattgccat gctgctgccg gccacctatg
     8881 ccttgccgcc ggtgtcggcg ctgatcatgc tggctggcat ttactacggc gcgcagtacg
     8941 gcggctcgac caccgcgatt ctggtgaatt tgccaggcga ggcatcttcg gtggtgacgg
     9001 tgattgacgg ccaccagatg gcgcgcaagg gccgcgccgg gccagcgctg gcggcagccg
     9061 ggctgggttc gttttttgcc ggttgtgttg gcacgctgac tctggcggcg tttgcgacac
     9121 ccctgaccga agtcgctttc aagtttgggg cggccgagta tttcgcgctg atggtgctcg
     9181 gcctggtggg tgcggtggta ctggcttcgg gctcgctgct caaggcggtg gcgatgattg
     9241 tgctgggctt gctgctgggc ctggtcggta ccgatgtcaa ttccggggtg gtgcgttttg
     9301 cgtttgacat accggaactc accgacggca ttggcttcat cgtgatcgcc atgggcatct
     9361 ttggctacgg cgagatcatt gccaacctgt cgatcaaagc ggcgcagcgt gaggtgtttg
     9421 cccacaaggt gcatggcctg atgccgaccc gtgaagactt ccggcgcatg tggccagccg
     9481 tgttgcgtgg caccgccatt ggctccatgc tgggcatctt gcccggcggc ggtgccttgc
     9541 tggcggcttt tgccgcctac agcgttgaga aaaaaaccag gctgcaagcc ggcgagctgc
     9601 cgtttggcca aggcaatatc cggggtgtgg cagcgccaga gtcggccaac aacgcgggcg
     9661 cgcagacttc ctttattccg ctgctgacgc tgggcattcc gcccaacccg gtgatggcgc
     9721 tgatggtcgg tgccatgacc atccacaaca tccagccggg cccgcaggtg atga
//
LOCUS       DAOSLL010000098         9742 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6739_length_9742_cov_5.491071, whole genome shotgun sequence.
ACCESSION   DAOSLL010000098 DAOSLL010000000
VERSION     DAOSLL010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9742
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6739_length_9742_cov_5.491071"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1013
                     /gene="trpD"
                     /locus_tag="PLB25_06025"
     CDS             <1..1013
                     /gene="trpD"
                     /locus_tag="PLB25_06025"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOE41170.1"
                     /translation="RTIEHREIFHDEMLHLMRLIMNGELSPVMTASIITGLRVKKETI
                     GEITAAAQVMREFSTKVQVADKTHLVDIVGTGGDGSHTFNISTCAMFVAAAAGARVSK
                     HGGRSVSSKSGSADVMESLGVNINLKPEAIAKCIEQVGVGFMFAPNHHPAMKNVAPVR
                     RELGVKTIFNLLGPLTNPADAPNILMGVFHSDLVGIQVRALERLGCEHAVVVYGRDGM
                     DEVSLGAATMVGELKNGKITEYEIHPEDFGMVMASNRSFKVETPDQSRAMLLGVLDNV
                     SGPAKEIVVLNAGVALYAANVTDSIAAGISKARAAIESGASRAKLDALVAASKALSEA
                     AA"
     gene            1010..1147
                     /locus_tag="PLB25_06030"
     CDS             1010..1147
                     /locus_tag="PLB25_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41171.1"
                     /translation="MSLLNSPEAWIAIGISVLFIATGLVMHRVFVNILKNGSQEPPKN
                     D"
     gene            1140..1934
                     /gene="trpC"
                     /locus_tag="PLB25_06035"
     CDS             1140..1934
                     /gene="trpC"
                     /locus_tag="PLB25_06035"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HOE41172.1"
                     /translation="MTDILEQIVAVKRAEVAAATQRKSLAVMRADAESRVLTRDFVAA
                     LRTKIAAGQPAVIAEVKKASPSKGVLRADFIAADIAQSYAEYGAACLSVLTDVQFFQG
                     CVDFLKQARASCQLPVLRKDFMVDAYQIYESRAMGADAILLIAACLDDAQMKEFEAIA
                     RSLDMAVLVEVHDAAELVRALKLKTPLIGVNNRNLKTFEVSLDTTLTLMRDIPADRLL
                     VCESGIHSRDDVLRMGAAGVNAFLVGEAFMRADEPGKALAALFGPA"
     gene            1964..2671
                     /locus_tag="PLB25_06040"
     CDS             1964..2671
                     /locus_tag="PLB25_06040"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOE41173.1"
                     /translation="MKPATQLATADPSDWPVAQGWQALIAHFFESSQGLALLTFLQQR
                     LDAGAIVFPPQPLRALELTAPESVRVVILGQDPYHGRGQAEGLAFSVAAGVPLPPSLR
                     NIFKELQRDLGEAPPAFPNPGGSLVRWARHGVLLLNTCMTVEEGQAASHAGKGWEALT
                     DAIIEQVSQGPARLVFMLWGSHAQSKRVLIDAARHLILCANHPSPLSALRPPVPFIGC
                     GHFSQARAWRDQHQRPG"
     gene            complement(2852..3622)
                     /locus_tag="PLB25_06045"
     CDS             complement(2852..3622)
                     /locus_tag="PLB25_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HOE41174.1"
                     /translation="MNFENLLAYDHYGYAFVALVGTGVAAAAILWLLQKSPWSRWIRS
                     LHGVAPPFINIIGVLFGLTLAFIANDTWNAHDKATHAIYREADALRSLNLLAQKLSPP
                     LRDQLQQALRDVARSSADEWPWLARRQSSQHATDCANTLLLLLASQELAQQAGANVQA
                     MMLKKASEMRDDRNLRIGLSQMHLNPLKWLGMAFLGFLTLLSITAVHLENPRAARVAI
                     ILFALSAAPTAAIMLIQGNPFQQPSFVSAQPILAALLP"
     gene            3657..3887
                     /locus_tag="PLB25_06050"
     CDS             3657..3887
                     /locus_tag="PLB25_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41175.1"
                     /translation="MGKFAAPNFQSTQGRWGGIAHLAAMNGVRLIVVIIKHPKPPGFC
                     GAETAGITHIGCLPCQNQPLPGRAFGRANASP"
     gene            4093..6138
                     /locus_tag="PLB25_06055"
     CDS             4093..6138
                     /locus_tag="PLB25_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009242108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HOE41176.1"
                     /translation="MYELHGTIAVVSLNKAPLNRLDAALRQHIASAVRQAGSDPAVSA
                     LVLMGNARAFASEADGIESGAPGQATEPELGQLCEQIESCQKPVVAAISGVALGGGLE
                     LALACHGRVAQGSARLGLPDITLGLIPGAGGTQRLPRLAGLTTALAMLQSGAPQTAQQ
                     LAQSGLLDQVVSEELLAAALQRATDLAGQGAPLVKARDRLLSAAAVQQFVSEQKDQLT
                     PRQRLQDVYVAQLDALAAAGGDFAQGLRREQELRAQLRSGIQSKALRYQLFAEREASK
                     LPAVLQAAPRSVATIAIIGAGTMGAAIAICALDAGLQVILLEQDDAALQRGQQRVTGH
                     YQGRVAAGKMAADIAAGAAARLSPTIDWAQLKLADLVIEAVFEDLAVKQAVFRTIDAH
                     ARPGAVLATNTSYLDVDAIASATSRPQDVLGLHFFSPANVMKLLEVVRASQTAPAVVA
                     TGMALGNTLNKLPVLTGNAFGFIGNRIYNAYRNQCEFMLEDGAWPEDVDNALQAFGFA
                     MGPFAVADLSGLDIAWRMRKSLADTRDPRERYVTILDQLCEQGRLGRKAEAGYYTYAA
                     GKQVKTTDAVVRDIIAQASRQRGIERRPLLPAVIQRRALLAMVNEAALLLAAGVASRA
                     SDIDVVLTQGYGFPRWQGGPVFWARQQERRALAQDLQSLAAQSGHGFVPADLSVLLD"
     gene            6164..7366
                     /gene="pcaF"
                     /locus_tag="PLB25_06060"
     CDS             6164..7366
                     /gene="pcaF"
                     /locus_tag="PLB25_06060"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HOE41177.1"
                     /translation="MNQAFICDAIRTPIGRYGGALSSVRTDDLGAIPLKALMARNPSV
                     DWEAVTDVMYGCANQAGEDNRNVARMVSLLAGLPVAVPGVTLNRLCGSGLDAVGTAAR
                     AIKAREATLIIAGGVESMSRAPFVMPKMEIAFGRNNAVHDTTIGWRFVNRLMKERFGV
                     DSMPETAENVATEFNIERAAQDRMALASQQRALAAQQAGHLAREITAVTVAQKKGDAL
                     TVDQDEHPRETTLQALAKLKGVVRPDGSVTAGNASGVNDGACALVLADEATAASNGLT
                     PKARVVGMATAGVAPRIMGIGPAPATQKLLAQTGLTLGQMDVIELNEAFAAQGLAVLR
                     LLGLADDDARVNAWGGAIALGHPLGASGARLATTAVNRLHANGGRYALCTMCIGVGQG
                     IALILERV"
     gene            7458..7592
                     /locus_tag="PLB25_06065"
     CDS             7458..7592
                     /locus_tag="PLB25_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41178.1"
                     /translation="MPYPKMDHQAVADSGPSDVRQLVTSQDTIDLLQIACLGHLSHFA
                     "
     gene            complement(9521..>9742)
                     /locus_tag="PLB25_06070"
     CDS             complement(9521..>9742)
                     /locus_tag="PLB25_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41179.1"
                     /translation="LVLVLVLVLVLVLVLVLVRVRQQPQLLAHWPGSMRTRRRADAGL
                     MGRLYRHNPRRCSPECRQSGSVARRQTSP"
BASE COUNT         1880 a         2925 c         3027 g         1910 t
ORIGIN      
        1 agcgcaccat cgagcaccgc gaaatcttcc acgacgagat gctgcacctg atgcggctca
       61 tcatgaatgg cgaactctcg ccggtcatga ccgcgtccat catcaccggc ctgcgcgtga
      121 agaaggaaac cattggcgag atcaccgccg ccgcgcaggt gatgcgcgag ttttccacca
      181 aggtgcaggt ggccgacaaa acccacctgg tggacattgt tggtaccggt ggcgatggct
      241 cgcacacctt caacatttcg acctgcgcca tgtttgtcgc ggcagcggca ggcgctcggg
      301 tcagcaagca tggcggccga agcgtcagca gcaagagcgg cagcgccgat gtgatggagt
      361 cgctcggggt caacatcaac ctcaagcccg aagccattgc caaatgcatc gagcaggtcg
      421 gggtcggctt catgtttgcg ccaaatcacc atccggccat gaagaacgtg gccccggtgc
      481 ggcgcgaact gggggtcaag accatcttca acctgctcgg gccgctgacc aacccggccg
      541 atgcgccgaa cattctgatg ggcgtgtttc actccgacct ggtcggcatt caagtgcgcg
      601 cgctggagcg tctgggttgc gaacatgcgg tggtggtgta tggccgcgac ggcatggatg
      661 aggtgtcgct gggcgcagcc accatggttg gtgaactgaa aaacggcaag atcaccgagt
      721 acgaaattca cccggaagac ttcggcatgg tgatggcgag caaccgctcc ttcaaggtcg
      781 aaacccccga tcagtcgcgc gccatgctgc tcggtgtgct cgacaacgtg tcaggcccgg
      841 cgaaagaaat cgtcgtgcta aacgccggtg ttgcgctgta cgctgccaat gtgaccgatt
      901 cgattgcagc cggtatcagc aaagcgcgcg cagctattga atcaggcgct tcgcgggcca
      961 agctggatgc cctggtggcg gcctcaaagg cgctgagcga ggccgcagca tgagcctgct
     1021 caatagcccc gaggcctgga ttgcgattgg catctcggtg ctgttcatcg caaccgggct
     1081 ggtgatgcac cgcgtgtttg tcaacattct caaaaatggc tcacaggagc cacccaaaaa
     1141 tgactgatat tctggaacaa attgtcgccg tcaaacgtgc cgaagtggcc gccgcgacgc
     1201 agcgcaaatc actggccgtg atgcgcgcag atgccgaaag ccgggtgctg acgcgtgatt
     1261 ttgtcgctgc cttgcgcacc aaaattgccg ctggtcagcc agcggtgatc gccgaggtca
     1321 aaaaagccag tccgtccaaa ggcgtgctgc gcgccgactt cattgccgcc gacattgcgc
     1381 aaagttatgc cgaatatggc gcggcctgtc tgtcggtgct gacggacgtg cagttttttc
     1441 agggttgcgt tgattttctg aagcaggcgc gggcctcttg ccagttgccg gtgctgcgca
     1501 aggacttcat ggtcgatgcc tatcaaatct acgagtcacg ggcgatgggt gcggacgcca
     1561 ttctgctgat tgcggcttgc ctggacgatg cgcagatgaa ggagttcgaa gccattgccc
     1621 gcagccttga catggcggtg ctggtggagg tgcacgacgc ggccgaactg gtgcgcgccc
     1681 tgaagctcaa aaccccgctg atcggtgtca acaaccgcaa cctcaaaacc tttgaagtgt
     1741 cgctcgacac cacgctgacg ctgatgcgag acattcccgc cgaccggctg ctggtgtgcg
     1801 aaagcggtat ccacagccgc gacgatgtgc tgcgcatggg cgcggccggg gtcaatgcgt
     1861 ttctggttgg cgaggccttc atgcgcgccg acgagccggg caaggcactg gcggccttgt
     1921 tcggaccagc ttgactggcc tttgttgagc tggccgctgc cccatgaagc ccgccaccca
     1981 gttagccacg gctgacccgt ctgattggcc tgtagcccaa ggctggcaag ccttgatagc
     2041 tcactttttt gaatcaagtc aggggcttgc gctgctgacg ttcctgcagc agcggcttga
     2101 tgccggggcc atcgtgtttc cgcctcaacc cttgcgtgcg ctggaattga ctgcgccgga
     2161 gtcggtccgc gtggtgattt tgggccagga cccctatcac gggcgtggcc aggctgaagg
     2221 cctggcgttc tcggtggctg ctggcgtgcc tttgccgcca tcgctgcgca acattttcaa
     2281 ggaactgcag cgcgacctgg gtgaagcacc accggcgttt cccaaccctg gcggcagcct
     2341 ggtgcgctgg gcccggcatg gggtgctgct gttgaacacc tgtatgacgg tggaagaagg
     2401 acaagccgcc agccatgccg gcaaaggctg ggaggccctg accgatgcca tcatcgagca
     2461 ggtgtcccaa ggcccggcac ggctggtgtt catgctgtgg ggcagccatg cgcaaagcaa
     2521 acgggtgctg atagatgccg cacgccacct catcctgtgt gccaaccacc catcgccgct
     2581 gtcagcgctg cgtccaccgg taccctttat cggctgtggc catttctcgc aagccagggc
     2641 ctggcgcgat cagcaccaac gcccagggta gctggccggt cggtttgcct tgcaggtttt
     2701 ttttgcctgc agcgtggtaa ccggaccgcg cccggtgctg cgccacagtt caccccgcca
     2761 tcgggtctgg atcaggcaat cggctaccga ccaatgagag cgaacggttg tgccgcgtga
     2821 gtgaccactg cgcaacaagg gaccggcccg gtcagggcag cagggccgca agaatgggct
     2881 gcgccgagac aaacgaaggt tgctgaaacg gattgccctg aatcagcatg atcgccgccg
     2941 tgggtgccgc tgacagggca aacaagatga tggcgacgcg tgcggcacgc gggttttcaa
     3001 ggtgcaccgc agtaatcgac agcagtgtca aaaaccccag aaaagccatg cccagccact
     3061 tcagcgggtt aaggtgcatc tggctcaagc caatgcgcaa attgcggtca tcgcgcattt
     3121 cagaggcctt tttgagcatc atggcctgaa cattcgcgcc agcctgttgc gccagttcct
     3181 ggctggccag cagcaacagc aaggtgttgg cgcagtcggt ggcgtgctgg cttgattggc
     3241 ggcgcgccag ccacggccat tcgtcggcgc tcgaccgggc cacgtcacgc agcgcctgct
     3301 gcaactggtc acgcagcggc gggctcaatt tctgcgccag cagattgagg ctgcgcagtg
     3361 catcggcctc gcggtaaatc gcgtgggttg ccttgtcgtg agcgttccag gtgtcgttgg
     3421 caatgaatgc cagcgtcagg ccaaacagca caccgatgat gttgatgaag ggcggtgcca
     3481 cgccatgcaa ggaccgaatc cagcggctcc agggtgactt ttgcagcagc cacaaaatgg
     3541 ctgctgcggc aacaccggtg ccgacaagcg ccacaaaggc atagccgtag tggtcgtagg
     3601 caagcaagtt ttcaaagttc atgcccgatt gtctcagtcc tggatgctcc tgttttgtgg
     3661 gcaagtttgc ggcaccaaat tttcaatcga cgcagggccg ttggggcggc attgcgcatc
     3721 tggcagccat gaacggtgtc aggctgatcg tcgtcatcat caaacacccc aagccacccg
     3781 gcttttgcgg cgcagagacc gcaggcatca cgcacattgg ctgcttgccg tgccagaatc
     3841 agccgctgcc cggtcgtgcc ttcgggcgtg caaacgccag cccctaagcc ggacaggccg
     3901 gaattggacg gccttcgtcg cgagggcgca actcctacac aaacagtcgg cgccaatttg
     3961 aatttgtagg agtcgcgccc tcgcgacgaa tgacttggaa tgtttgaatt cttcgcatga
     4021 ttcgcaaaga cttgacttgt tagagactaa aagtgactgt cgtttggctg cgctatctca
     4081 ggaccaccca ccatgtatga acttcacggc acgattgccg tcgtctcgct caacaaagcg
     4141 ccgctcaacc gtttggacgc ggccctgcgc caacacattg cctccgccgt gcgtcaagcc
     4201 gggtcagacc cggccgtcag cgcgctggtg ctgatgggca acgcccgggc ctttgccagc
     4261 gaggccgatg gcattgagtc aggcgcacca gggcaagcga ccgaacccga gctgggccaa
     4321 ctgtgtgaac agattgagtc ctgccagaag ccggtggtcg ctgccatcag cggtgtggct
     4381 ctgggtggcg gtctggaact ggcgctggcc tgtcatggcc gggtggcgca gggcagcgcc
     4441 aggctgggcc tgcctgacat cacgctcgga ctgattcccg gtgctggtgg cacacagcgt
     4501 ctgccacgcc tggctggcct gaccacggcg ctggccatgc tccaaagcgg cgcgccgcag
     4561 accgcccaac aactggcgca gtctggcttg ctcgaccagg tggtgtctga ggaactgctg
     4621 gcagcagccc tgcagcgtgc caccgatctc gcaggtcagg gtgccccgct agtcaaggcg
     4681 cgcgaccgct tgctcagtgc ggcggcagtg cagcaatttg tcagtgagca gaaagaccag
     4741 ctcacgcccc gccaacgcct gcaggatgtt tatgtcgccc agcttgatgc gctggccgct
     4801 gcgggcggcg actttgccca aggcttgcgt cgcgagcagg aactgcgtgc acagttgcgg
     4861 tccggcatcc agtccaaagc cttgcgatac cagttatttg ccgagcgtga agccagcaaa
     4921 ttgcctgctg tgctgcaagc ggcacctcgt tcagtggcca ccatcgccat cattggcgct
     4981 ggcaccatgg gtgcagcaat tgccatttgc gcgctggatg ccgggctgca ggtgattttg
     5041 ctggagcaag acgatgccgc cttgcagcgc ggccagcagc gtgtgaccgg gcattaccag
     5101 ggccgcgttg ccgccggaaa aatggccgct gacatcgctg caggggccgc agcccgcctg
     5161 tcgcccacca tcgattgggc gcaactgaag ctggctgatc tggtgattga agcagtgttt
     5221 gaagacctgg ccgtcaagca agcggtgttc aggaccatcg atgcccatgc ccgccctggc
     5281 gcggtgctgg ccaccaacac ctcgtacctg gacgtggatg cgattgccag tgccaccagc
     5341 cgtccccagg atgtgctggg cctgcacttt ttcagcccgg ccaacgtcat gaagctgctc
     5401 gaagtggtgc gcgccagcca gaccgcacct gcggtggtgg ccactggcat ggccctgggc
     5461 aacacgctca acaagctgcc ggtgctcact ggcaatgcct ttggttttat cggcaaccgc
     5521 atctataacg cttaccgcaa tcagtgtgaa ttcatgctcg aagacggtgc ctggcccgaa
     5581 gacgtggaca acgcgctgca ggcctttggc tttgccatgg ggccgtttgc agtggctgac
     5641 ctgtccgggc tggacattgc ctggcgcatg cgcaagtcgc tggccgacac gcgtgacccg
     5701 cgtgagcgct atgtgaccat cctcgaccag ttgtgcgaac agggtcgact ggggcgcaag
     5761 gccgaggctg gctattacac ctacgcggca ggcaagcagg tcaaaaccac cgatgccgtg
     5821 gtgcgcgaca tcattgccca ggcatcaagg cagcgcggca tcgagcggcg cccgcttttg
     5881 cctgccgtca tccagcgccg tgccttgctg gccatggtca acgaagcagc cctgctgctg
     5941 gcagcgggcg tggccagccg ggccagcgac atcgacgtgg tgctgaccca gggctatggt
     6001 tttccccgct ggcaaggtgg cccggtgttc tgggcgcgcc agcaagagcg ccgcgcgctg
     6061 gcgcaagacc tgcaaagcct ggccgcacaa tcgggtcacg gctttgtgcc cgctgacctg
     6121 tccgtgctgc tggactgatc cctttgactg aaagaccaat cacatgaacc aggcttttat
     6181 ttgtgatgcc attcgtaccc cgattggccg ctacggcggt gccttgagca gcgtgcgcac
     6241 cgacgacctg ggcgccattc ccctgaaggc cttgatggcc cgcaaccctt cggtggattg
     6301 ggaggcagtc accgacgtga tgtacggctg cgccaaccag gccggtgaag acaaccgcaa
     6361 tgtggcgcgc atggtgagtt tgctggccgg gttgccggtg gcggtgccag gtgtcacgtt
     6421 gaaccgcctg tgtggctctg gcctggatgc ggttggcact gcagcccgcg ccatcaaagc
     6481 gcgggaagct acgttaatca tagctggtgg ggttgagagc atgagccgtg cgccattcgt
     6541 catgcccaag atggagatcg cctttggacg caacaacgcg gtgcatgaca ccaccattgg
     6601 ctggcgtttt gtcaaccggc tgatgaaaga gcgctttggc gtggacagca tgcccgagac
     6661 cgctgaaaac gtcgccaccg aattcaacat cgaacgcgct gcgcaggatc gcatggcgct
     6721 ggccagccag cagcgtgcgt tggccgccca acaagccggc cacctggcgc gtgaaatcac
     6781 ggccgtaacg gttgcgcaaa aaaaaggcga tgccctgact gttgatcagg atgaacatcc
     6841 gcgcgaaacg actctgcagg cgctggccaa actcaagggt gtggtgcgcc cggacggctc
     6901 ggtcaccgct ggcaacgcaa gtggtgtcaa cgacggggct tgcgccttgg tgctggccga
     6961 tgaagccacc gctgccagca atggcctgac ccccaaagca agggtggtcg gcatggccac
     7021 tgcgggtgtg gcaccgcgca tcatgggcat tggcccggca ccggcaaccc aaaagctgct
     7081 ggcgcaaacc ggcctgacgc tggggcaaat ggatgtgatc gaactcaacg aagcctttgc
     7141 cgcgcagggt ttggcggtat tgcgtctgct cggcctggcc gacgatgacg cacgcgtgaa
     7201 cgcctggggc ggtgcgattg cgctgggtca tccgctcggg gccagcggcg cgcgactggc
     7261 cacgaccgcc gtcaaccgcc tgcacgccaa tggtggtcgc tacgcgctgt gcaccatgtg
     7321 catcggtgtc gggcaaggga ttgcgctgat tcttgagcgg gtttgaaaat gctattagtt
     7381 gtatagctag cagcgcatgt atatcaaggc tcagaggcac ttttggctta tcaattgaca
     7441 aggccctgaa actcaggatg ccctacccca aaatggacca ccaggcggtg gccgacagcg
     7501 gcccgtcaga tgtccggcaa cttgtcacga gccaggacac cattgacctg ctgcagatcg
     7561 catgcttggg tcatttgagc cactttgcct gagccaaccg tcaaccatga ggcgcgcatc
     7621 cacagaacaa attggttttc cggctgagtt gttgagtgcc cgtcatgtcc gccttcgtga
     7681 caatgatggc gcggccatgg gcgcaaattt gccctggaag ccgggcagac ttgcggcatt
     7741 gatcgcgcaa cccgaccccg gtgaggggtg ccggattcac cggccccgag ccagttgccg
     7801 gccaagatgg cggtgcaaaa ttggccgctg cggtcgtttc gcagccgatg cgacacatgt
     7861 ctgactggct catggcacgt caatgaccct gcttgtcgct ttcctgccac gcgctgaagg
     7921 gtgacccgac gcttggtgaa agattggaca ttgccaagcg gtgcaatgtg cctttgcggc
     7981 ggttctctgc cccaacttgg cgcagatcag ccgatgcggg cccagttgat ccgtcgaacc
     8041 gacctgagcc tgacaaacaa cgtcaactcc cttgcgcctt gccgaaaact tctgatcaac
     8101 gcatctcgca gcgcctgtgc ccagcttggg cagtgcaggg cggctgcggc gcatcagtta
     8161 tgtgaaaatg ccaccaagcg cgctggcagc ggcaattttg gagcgcttgt caatgttgtc
     8221 cgacacaggc atctcttgtt gggtcaccgt ttcacctgtt tatatagtgt cttttgatcg
     8281 ttaattgatt gactgatgtt tcgcagccca ctcatgttag gcttgacgaa tggaaaaagc
     8341 tgacctggat ttgtgcccgg atgattcgtt ggacaccctt ggtgtggcaa cgacgttggg
     8401 cttggtggca aagatgccaa gtgatgctgg ccacgaccag ggtacgcggt ttgcatcggt
     8461 gcagcagttc tccacggtcc tgtggcaacc ggtcaagtcg gtggccagtt caattgagcg
     8521 tgacgatggt gtgttggtct ctgacaacac cattcaagag aatgcctgga ccgatgagag
     8581 caggttggtt tgctgccatc ttgaccattg cagtgctcct gccgtcaccg gggccaagcc
     8641 aggaacgagc cggactgatg gattaagcag cgcgaatcga ccccacaaag ggatgccgga
     8701 tttgccggtc catcgcccag ttgcaggcca aaaggccagt gcaaaatggg ccgcttcggt
     8761 cggttcggcg gcttcggtcg gttcgccgct ctgccacaag tctgacgcgc cgcaggtcaa
     8821 cgcattgcag tgccacaacg gtaactcaat cgatctggcg tttgggctgg caaggttgcc
     8881 gatccagtcc cgcgaccttg tgacaaaagc gctcagccaa caaagtgacc agaccaaaag
     8941 cgcggtgatg cctgagatga ccgaagcctg tgttaaggac acttcaaagt ttcgctttgt
     9001 gttgcagcac agttggcttt cttcaaaaga gcgcttcagt ttcatcgccg ccaagggtgg
     9061 agatgtgcca gtttggccaa gtccccggcg cgaacactgc gcattcaatg caaacaagct
     9121 ttcatggcca ttgatgccac gttcaggctc aaatacctgc gcagcacgaa ccagctcaat
     9181 ccgattgcgc tttgtcacaa gccactcatc aacgcctctc gcatcgcctg cgcgcagctt
     9241 catctgcttt gggcggctgc gtaacatccg tgattttgta tgactctctt gaatcgaccc
     9301 ggttttttga cgctttcgat ggccctcttg ctgggttgtt gtttgaccgc gagcgcacaa
     9361 acaacaaccg ctgcaaagga ccttcagaat gcttgccttg tctcggatat cccggccatc
     9421 ggccagataa gcgtctcctg gaccggcgag tgtgttgaca gcaaagccag cggtgtgggt
     9481 gatttactgg gctttggcgc ggggaaactg cgctacatcc tcaggggctc gtttgtcggc
     9541 gggcgactga cccggattga cgacattcgg gactgcagcg ccggggattg tgacgataga
     9601 gtcgccccat caatcctgcg tcagcacgcc gcctggtacg catcgagccc ggccagtgcg
     9661 caagcagttg cggctgctgt cgcacccgca ccagcaccag caccagcacc agcaccagca
     9721 ccagcaccag caccagcacc ag
//
LOCUS       DAOSLL010000099         9718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6764_length_9718_cov_4.500983, whole genome shotgun sequence.
ACCESSION   DAOSLL010000099 DAOSLL010000000
VERSION     DAOSLL010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9718
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6764_length_9718_cov_4.500983"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="PLB25_06075"
     CDS             <1..262
                     /locus_tag="PLB25_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41180.1"
                     /translation="PLNVGAAGGGECFLNTGDLIGFSRLPQVGSTTAEMKVIASGMNS
                     CRPDAVVLVPLTELQEMLNGFSQRVEDSIRRVSACSTAGGRC"
     gene            312..785
                     /locus_tag="PLB25_06080"
     CDS             312..785
                     /locus_tag="PLB25_06080"
                     /inference="COORDINATES: protein motif:HMM:NF024637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOE41181.1"
                     /translation="MFCINCGKSIEPTAAFCTHCGTHQYAEPAPSAPSAPSAPSATVF
                     PAYVSPEAPSPSMTAHAAAAPASGGKAALLAGAVVLGLSAAGGLGYWGWSTKMADVEA
                     TQKQAIEEVARKAADEEQRRIAAERAVEAAEIKAAQAIMDKQIAAEEAQALAQTR"
     gene            931..1992
                     /locus_tag="PLB25_06085"
     CDS             931..1992
                     /locus_tag="PLB25_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOE41182.1"
                     /translation="MNTVRLGQSDLFASRICLGTMTFGEQVDEATAFDIMDRSLERGV
                     NFLDTAEMYAVPPRAETFNATEKIIGNWLAKRPGVRQSMLIASKVAGPSRAMKWIRNG
                     SADLTGADILQACDDSLQRLQTDVIDLYQIHWPVRSVPMFGSLYYQRSDAQVTSIHAQ
                     LEALGKLVKAGKVRAVGLSNETPYGVHEFVRLAEQHGLPRVATVQNGYSLLNRSVENA
                     LDETLHQLNVSLLAYSPLAFGLLTGKYDASGLTGPNAPAQARLTRFGSTRKQRWGRPE
                     ALAAARRYSALACAHGLTPLQLALAFCYTRWQVASTIIGVTSVAQLDECLDVWGRTLP
                     EQALSQIDAIRWEMRDPAQ"
     gene            2312..2710
                     /locus_tag="PLB25_06090"
     CDS             2312..2710
                     /locus_tag="PLB25_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOE41183.1"
                     /translation="MALQTPVEYDDASAAVKAVYDDIMATRNTDWVNNFWKVLAHHPQ
                     TLQRVWSNIKQVMGNGALDPLTKELIYVAVSVTNNCDYCIASHTAGAKAKGMTDEQLG
                     ELLAVVGLANETNRLAVGYGVPIDERFAAH"
     gene            complement(2976..3287)
                     /locus_tag="PLB25_06095"
     CDS             complement(2976..3287)
                     /locus_tag="PLB25_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41184.1"
                     /translation="MNPNRTSFTLLQASQNNPHLAKLMAIQKASQDRLQAIKPLMPTA
                     LQASVQCGPLEDGIWCLLLSNNTMAAKLRQLLPALEAHLRVKNLEVKSIRLKVRRQDK
                     H"
     gene            3308..4255
                     /locus_tag="PLB25_06100"
     CDS             3308..4255
                     /locus_tag="PLB25_06100"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOE41185.1"
                     /translation="MQLIITDAWLAKSRALHLTGIQLVVVFVLSSFVLMLSALGLYHW
                     VFVKGAREGWPIIGSLVKLVAKDEFAQRDRFLRENLDLMARQLGELQAKLTQLESLGE
                     RVSGLAGVNAAQVKGAAGQGGVQIGDRALTLAEIKTAMDDLENLTHQRTDLLTVLESR
                     LFEKKMKDLMVPTQRPVTQGEVGSTFGWRLDPITGRSALHTGLDFPMATGTAILSAAG
                     GVVVAQEYHPEYGSMVEIDHGNDLITRYAHASRVLVKKGDLIRRGQKIAEVGTSGRST
                     GAHLHFEVLVQGIPQDPQKFLLAGQHLGAPQMATATTAK"
     gene            4426..7170
                     /gene="secA"
                     /locus_tag="PLB25_06105"
     CDS             4426..7170
                     /gene="secA"
                     /locus_tag="PLB25_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HOE41186.1"
                     /translation="MATNFLTKIFGSRNDRLLKKYRNAISKINALEAGFEQLSDAALL
                     AKTDELKQRITQGATLDDLLPEAFAVVREGSKRVMKMRHFDVQLLGGMSLHYGKISEM
                     GTGEGKTLTATLPVYLNALTGNGVHVVTVNDYLANRDAQWMGKLYNFLGLSVGINLPN
                     MSREEKQAAYRADITYGTNNEYGFDYLRDNMVYEAADRVQRGLNYAIVDEVDSILIDE
                     ARTPLIISGQSEDQTNLYQGIKLIVPQLEKQEGEADPHTGEGITKPGDFTIDEKGHQV
                     FLTERGHENAERILFEMGLIPEGASLYDPANITLMHHLYAALRAQHLYHRDQHYVVQA
                     GEVVIVDEFTGRLMTGRRWSEGLHQAVEAKEGVAIQPENQTMASITFQNYFRLYGKLA
                     GMTGTADTEAYEFQEIYGLETVVIPPNQPSRRDDQLDRVYKTTQEKYQAAIADIRECY
                     ERGQPVLVGTTSIENSEVIATLLEKEKLPHQVLNAKQHAREADIVAQAGRSRMITIAT
                     NMAGRGTDIVLGGNISKLIEAIETDEALDAARKQQQLEALRTQWTQDHEMVTSLGGLR
                     IIATERHESRRIDNQLRGRSGRQGDPGSSRFYLSLDDSLMRIFAGDRVKAIMDRLKMP
                     EGEAIEAGIVTRSIESAQRKVEARNFDMRKQLLEYDDVSNDQRKVIYQQRNEILDASD
                     LSAQVQSLREGCFHDIVRQYVPVESVEEQWSLPALEKVLQDEWQITLPLQHQVNAASA
                     ISDFDLVDTVRVHANQLFEAKVALVGNQNFTQFERMVLLQSIDTNWRDHLSSLDYLRQ
                     GIHLRGYAQKQPKQEYKREAFELFGQMLDAVKNEVTKVLMTVRVQSNEEVERAAAEME
                     SRAEHISNVTYSAPTETGEVHTTVDNTTVAKAVPRVGRNEPCPCGSGKKYKQCHGKLA
                     "
     gene            7255..8484
                     /gene="argJ"
                     /locus_tag="PLB25_06110"
     CDS             7255..8484
                     /gene="argJ"
                     /locus_tag="PLB25_06110"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HOE41187.1"
                     /translation="MAVNLTTPNPAELCPVAGLRIGVTEAGIRKANRKDLTVVLIDQG
                     ASVAGVFTQNRFCAAPVQVCREHLADAQGIRAMLINTGNANAGTGADGLARARRTCQA
                     LADRLGLTAQQVLPFSTGVIMETLPIERIEAGLDAALLDARAGNWQRAAEAIMTTDTV
                     PKAFGAQVMVDGALVSITGISKGAGMIRPNMATMLGFMATDACVNPALMKQLATELAE
                     GSFNRVTVDGDTSTNDSFVVIATNRAAHPPIDSLASPAGQALKAAMLGVARNLAQAIV
                     RDGEGATKFISIEVAGGQSQAECRQVAYAIAHSPLVKTAFFASDPNLGRILAAVGYAG
                     IADLDQTRIDLYLDDVLVVVQGGRNPAYREEDGQRVMQQSEITVRVLLGRGSATDTVW
                     TCDLSHDYVSINADYRS"
     gene            8481..9374
                     /locus_tag="PLB25_06115"
     CDS             8481..9374
                     /locus_tag="PLB25_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE41188.1"
                     /translation="MNPSFEHLLARAEQLIARIEAVLPQPLAAPDWTAAHAYRYRKRS
                     AGRSSLEPVRHIGVMKLSDLKEIGAQKEKIQRNTLQFVRGDLANNVLLTGARGTGKSS
                     LIRACLNEYAHLGLRLIEVDKADLVDLPDIVDLVSDRPEKFIVFCDDLSFEDGEPGYK
                     ALKSILDGSVAATTPNVLIYATSNRRHLLPEHMKDNLSYTHSPDGEVHPGEVVEEKIS
                     LSERFGLWVSFYSFNQDEYLAIVAQWLSSFGVAASVIEHARAEALVWALERGARSGRV
                     AYQFARDFAGRQSAAPGAAQP"
     gene            9371..>9718
                     /locus_tag="PLB25_06120"
     CDS             9371..>9718
                     /locus_tag="PLB25_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HOE41189.1"
                     /translation="MTEPRKPLVADGHEARAGGPVRPVLDVAVGVLIRSNGDFLLTSR
                     PAGKVYTGYWEFPGGKLEAGETVEQALARELQEELGIQTGQIVRWQSELVDYPHALVR
                     LHFCKVFDWTGQLQ"
BASE COUNT         2051 a         2727 c         2950 g         1990 t
ORIGIN      
        1 gcccctgaac gtcggggctg ctgggggcgg ggaatgcttc ctcaatacgg gcgatctgat
       61 cggcttttcc aggcttccac aagtgggcag taccactgcc gaaatgaagg tcattgccag
      121 tggcatgaac agttgcaggc ccgatgcagt ggtgctggtg ccactgacgg agttgcaaga
      181 gatgctcaac gggttcagtc agcgggttga ggacagcata aggcgggtca gtgcctgttc
      241 taccgcaggc ggtcgctgct agtggtgaag cggccaagcg tgtcggcatg ttttgaacat
      301 tgaggaaaac catgttttgt atcaattgcg gtaagtccat agagcccact gcagctttct
      361 gcacccactg cggcacccat caatacgctg agccagcgcc gtcagcgccg tcagcgccgt
      421 cagcaccgtc agcaacggtg ttcccggcct atgtttcgcc tgaggcccca tcgccgtcaa
      481 tgactgccca tgcagcagcg gccccggcaa gcggtggaaa agcggcattg ctggcaggtg
      541 cggtggtgct gggcttgtca gcggcgggag gtttggggta ctggggctgg agcaccaaga
      601 tggccgatgt tgaggcgacc cagaagcagg ccatcgaaga ggtcgcccgt aaagctgccg
      661 atgaagagca gcgcaggatt gctgccgaaa gagcggtgga ggctgccgag atcaaggccg
      721 cccaggccat catggacaag cagatcgctg ccgaggaagc ccaggctttg gcccagaccc
      781 gctgagtcca gcgtcgccaa ccggccttgt tgtcctggca tctggggccg cctggtctgc
      841 ttgctggcgc tttgaatcac cattcggtac gctgccaggg cgaaatcagt acactgccgg
      901 tttctgtctt tcaacgcctg attccgatcc atgaataccg ttcgacttgg tcaaagcgac
      961 ctgttcgcct cccgcatctg tctcggcacc atgacctttg gcgagcaggt tgatgaggcc
     1021 accgcctttg acatcatgga ccgttcgttg gagcgcggtg tcaactttct ggatactgca
     1081 gagatgtatg ccgtgccgcc cagggcagaa accttcaacg ccacggaaaa gatcattggc
     1141 aactggttgg ccaaacgccc tggtgtccgg caatcgatgc tgattgccag caaagttgcc
     1201 ggaccaagcc gggcgatgaa atggattcgc aatggcagtg cggatttgac cggtgccgac
     1261 atcctgcagg cctgcgacga cagcctgcag cgattgcaga ccgatgtgat tgatctctac
     1321 cagatccact ggccggtgcg cagtgtgccg atgtttggca gcctgtatta ccagcgcagc
     1381 gatgcccagg tcacgtcgat tcacgcgcag cttgaagcac ttggcaaact ggtgaaagcg
     1441 ggcaaagtcc gggccgtcgg tttgtccaac gaaaccccgt acggcgtgca tgaatttgtc
     1501 cgtttggccg agcagcacgg tttgccgcgt gtggcgacgg tgcaaaacgg ctatagcctg
     1561 cttaaccgca gtgtggaaaa cgcgcttgat gagaccctgc accagttgaa cgtgtccttg
     1621 ctggcttatt cgcccttggc ctttggcttg ctgaccggca agtatgacgc aagcggcctg
     1681 accggcccga atgcaccggc gcaagcgcgt ctgaccaggt ttgggtccac ccgcaagcag
     1741 cgctggggtc gcccggaggc gcttgcggct gccaggcgtt acagcgccct ggcgtgtgcg
     1801 catgggctga cgcccttgca gttggcgctg gcgttttgct acacccgatg gcaagttgcc
     1861 agcaccatca ttggcgtgac ttcggttgct cagcttgatg aatgtctgga tgtctggggc
     1921 cgcaccttgc cggaacaagc gctcagccag attgatgcca ttcgctggga aatgcgcgat
     1981 ccggcgcagt agcgttgcgg gcgatgccgc tgggctgtgg tccgcccaat cctcgccgac
     2041 acctgttttg ccaacatcag aacggtcaat caatactata ataatagtag ctgtcagcgc
     2101 atgctgcata agggctggaa gccaaaatga tcatgcgtgg ttgcaagggg ctgattttcc
     2161 catcgaccct gtcgctgtaa cagccggtcg gcatttgggt gccacaacac caggaaaatc
     2221 cggttcatca atctggttca gcatcggcaa gcagcattgt tgtcaaaatc agcattgctt
     2281 cgatccactt tacagttgaa acgcaaacat catggcactt caaactcctg tcgaatatga
     2341 cgacgcatcg gctgcagtca aagcggtcta tgacgacatc atggcaaccc gcaacaccga
     2401 ctgggtgaac aacttctgga aagttctggc gcatcacccg cagaccttgc aacgcgtctg
     2461 gagcaacatc aaacaggtca tgggcaatgg cgcacttgac ccgctgacca aggaactcat
     2521 ctacgtggca gtcagtgtca ccaacaattg cgactattgc attgcgtccc acacggctgg
     2581 cgcgaaagcc aaaggcatga ccgatgaaca acttggggaa cttcttgcgg ttgtcggatt
     2641 ggccaatgaa accaatcgtc tggcggttgg ctacggtgtg ccgatcgatg agcgctttgc
     2701 ggcacattga caagacaatt ccaggcgttg caggtcgccg ccaggttcat accagcgcaa
     2761 ccggtttagc gcaggttacg ctcaaggggt gggtgccctg gccgccaagc tgcattggtt
     2821 ggcagttatc atcgcgacaa ttcgaatcgt ttcattgtgt tgtgcaaatg gtacgaatcg
     2881 atgacgcgcc gtacttttga cggtactttg tttaaccaac atgaaccact ttttgaatca
     2941 atgaccggcg ttttgatgcg ggttcaacaa gctgcctagt gcttatcctg cctgcgcact
     3001 ttgagtcgaa ttgacttcac ttcaaggttt ttgacgcgca ggtgtgcttc gagtgctggc
     3061 agcagttgcc gcaatttggc cgccattgtg ttgttggaca gcaaaaggca ccagatgccg
     3121 tcttccagcg gtccgcattg cacgctggct tgaagcgccg tcggcatcaa tggcttgatg
     3181 gcttgcagcc ggtcttgtga ggctttctgg atcgccatca gtttggccag atgcgggttg
     3241 ttttgactgg cctgcagcaa ggtgaatgat gtgcggtttg ggttcatgcg ttttggaaag
     3301 agaacaaatg caattgatca tcaccgatgc ctggctggca aagtcccggg cactgcacct
     3361 gactggcatc cagttggttg tggtgtttgt gctgtcatca tttgtcttga tgctgtcggc
     3421 acttggcctg taccactggg tttttgtcaa gggcgcacgg gaaggctggc cgattatcgg
     3481 gtctttggtc aaactcgtgg cgaaagacga attcgcccag cgggaccgct ttcttcggga
     3541 aaatctggac ctgatggcgc gccagctcgg tgaacttcag gctaagttga cccagcttga
     3601 atcgctcggc gaacgggtgt caggcctggc tggcgtgaac gccgcacagg tcaagggcgc
     3661 agcggggcag ggcggggttc aaattggtga ccgtgccttg acgctggccg agatcaaaac
     3721 agcaatggat gatctggaga acctgaccca tcagcgaacc gacttgttga ccgtgctgga
     3781 atcgcgcttg tttgagaaaa agatgaaaga cctgatggtg ccaacacagc gaccggtgac
     3841 gcagggtgaa gtgggttcga cctttggctg gcggcttgac ccgatcaccg gacggtctgc
     3901 gttgcatacc gggcttgact ttccgatggc gacgggcaca gccattctgt ctgccgcagg
     3961 cggggtggtg gtggcccagg agtatcaccc cgaatacggg tccatggttg aaatcgacca
     4021 tggcaacgac ctgatcaccc gctacgcgca cgcctccagg gtattggtca aaaaaggtga
     4081 cttgatccgg cgcgggcaga aaattgccga agttggcacc agcggccgct caactggcgc
     4141 acatcttcat tttgaggtgc tggttcaggg cattccacaa gacccgcaga aattcttgct
     4201 tgccgggcaa catctcggag ctccgcagat ggccaccgca acaacggcca agtgaggttt
     4261 ttactggatc atctgctcaa aggtaaaatg gcagcaagtg gcttgcgcgg cccagacgcg
     4321 ctggagcttt tgttatcacc cccctgtccc catttcagtt agatcgtaaa aaaggactgc
     4381 gatgccggtt tgccttgttt ggcatccact gctcgccctg cattcatggc caccaatttc
     4441 ctgacaaaaa tcttcggcag tcgcaatgac cgcctgttaa aaaagtaccg taacgcgatc
     4501 agcaaaatca atgcgctgga ggctggcttc gagcaattgt ccgatgcggc actgcttgcc
     4561 aagaccgacg aactgaagca acgcatcacg cagggcgcca cgctcgatga tttgctgccc
     4621 gaggcctttg cagtggtgcg cgagggctcc aagcgggtca tgaagatgcg ccattttgac
     4681 gtgcagttgc tcggcggcat gtcactgcat tacgggaaaa tttccgaaat gggtactggc
     4741 gagggcaaga ccctgactgc caccctgccg gtttacctca atgccttgac cggcaacggg
     4801 gtccatgtgg tgacggtgaa tgactatctg gccaaccgtg atgcccaatg gatgggcaag
     4861 ctctacaact ttttggggtt gtcggtcggc atcaatttgc ccaatatgtc gcgcgaagaa
     4921 aagcaggcag cttaccgcgc cgacatcact tatggcacca acaacgagta tggatttgac
     4981 tacctgcgcg acaacatggt ctatgaggca gcagaccggg tgcagagggg tttgaactat
     5041 gcgattgttg atgaggtgga ctcgatcttg attgacgagg cgcgcacgcc tctgatcatc
     5101 agtgggcagt ccgaagacca gaccaacctg taccagggca tcaagctgat cgtgccgcag
     5161 cttgaaaagc aggaaggcga agccgatccg cacactggcg agggcattac caagcctggc
     5221 gatttcacca ttgacgaaaa gggccaccag gtttttctga ccgagcgcgg ccatgaaaac
     5281 gccgagcgca tcctgtttga aatgggcctg attcctgaag gtgccagcct gtatgacccg
     5341 gccaatatca cgctgatgca ccatctgtat gctgcgctgc gcgcccagca cctgtatcac
     5401 cgtgatcagc actatgtggt gcaggctggc gaggtggtga ttgttgatga gttcaccggt
     5461 cgcctgatga cagggcggcg ctggagcgaa ggcctgcacc aggcggtcga agccaaggag
     5521 ggtgtggcaa ttcagccaga aaaccagacc atggcctcga tcactttcca gaactatttc
     5581 cggctttacg gcaagctggc tggcatgacc ggtaccgccg acaccgaagc ctacgaattc
     5641 caggaaattt atggcctgga gaccgtggtg atcccgccca accagcccag ccgccgggat
     5701 gatcaactcg atcgggtgta caagaccacc caggaaaagt accaggctgc cattgccgac
     5761 atccgcgaat gttacgagcg cggacagccg gtgctggtgg gcaccacatc aatcgaaaat
     5821 tctgaggtga ttgcgacctt gctcgaaaaa gagaagctgc cgcaccaggt tctcaatgcc
     5881 aaacagcatg cccgtgaggc cgatatcgtg gcgcaggcgg gccgctccag gatgatcacg
     5941 attgccacca acatggccgg tcggggcacc gatattgtgc tgggtggcaa catcagcaag
     6001 ctgattgagg cgattgaaac cgatgaggca ctcgatgctg cccggaaaca gcagcagctt
     6061 gaagccctgc gcacccagtg gacgcaagac catgaaatgg tcaccagtct gggtggcctg
     6121 cgcatcattg ccaccgagcg gcatgagtcg cgccgcatcg acaaccagtt gcgtggccgc
     6181 tcgggtcgtc agggcgatcc gggttcatcg cggttctacc tgagtctgga cgactccctg
     6241 atgcgcatat ttgccggtga ccgtgtcaaa gccatcatgg accgcctcaa aatgcccgag
     6301 ggcgaagcca tcgaggccgg catcgtgaca cgctcgattg agagtgccca gcgcaaggtc
     6361 gaagcgcgca acttcgacat gcgcaagcag ttgctggaat atgacgacgt gtccaacgat
     6421 cagcgcaagg tgatttacca gcagcgcaac gagattctgg atgcaagcga cctgtcagcg
     6481 caggttcagt ccttgcgcga aggctgtttt cacgacatcg tgcgccagta tgtgccagtt
     6541 gagtcggtcg aggaacagtg gagcttgccg gcgcttgaaa aagtgctgca ggatgaatgg
     6601 cagataacct tacccttgca gcatcaggtc aacgcggcca gcgccatctc cgacttcgac
     6661 ctggtggaca ccgtgcgcgt gcatgccaat cagttgtttg aagccaaagt ggcactggtc
     6721 ggcaaccaaa acttcaccca gttcgagcgc atggtgctgc tgcaaagcat cgacaccaat
     6781 tggcgtgacc acctgagttc gctggattat ttgcgtcagg gcattcacct gcgcggctat
     6841 gcccaaaagc aacccaagca ggaatacaag cgcgaggcgt ttgagctgtt tggtcaaatg
     6901 ctcgatgcgg tcaagaacga ggttaccaag gtgttgatga cagtgcgggt tcagtccaac
     6961 gaggaagttg aacgcgctgc ggcagagatg gaaagccggg ccgaacatat ttccaacgtc
     7021 acctacagcg cacccacaga aactggcgaa gtacatacca ccgtggacaa cacgactgtg
     7081 gccaaggccg tgccgcgggt tggccgcaat gagccttgcc cgtgtggcag tggcaaaaaa
     7141 tacaagcaat gccacggcaa gctggcctga ccgcctgcgc tcacagatcg ggccgggtgc
     7201 ccgatttttt tcgattgagt ttccttcgtt tgtgttgaat attgaaagaa caccatggct
     7261 gtcaacctga ccacccccaa cccggcagaa ctgtgtcccg tggctggctt gcgcattggc
     7321 gtgaccgagg ccggcattcg caaggccaac cgaaaagacc tgacggtggt gctgattgac
     7381 cagggtgcct cggtggcggg cgttttcacc cagaaccgct tctgtgccgc gccagtccag
     7441 gtttgccgcg agcatctcgc agacgctcag ggcattcgtg ccatgctgat caacaccggc
     7501 aacgccaacg ccggtaccgg tgccgacggg ctggcgcggg cccggcgcac ctgtcaggcg
     7561 ttggcagatc gattggggct gacagcgcaa caggtgctgc cattttcgac cggtgtgatc
     7621 atggaaacac ttccaataga gcgcattgag gccggtctgg atgcggccct gctggatgcg
     7681 cgtgccggta actggcagcg tgccgccgaa gccatcatga ccacggacac cgttcccaag
     7741 gcgtttggcg cgcaagtgat ggtggatggc gcgctggtca gcatcaccgg catcagcaag
     7801 ggggctggca tgattcgccc gaacatggca accatgctgg gcttcatggc caccgatgcc
     7861 tgcgtcaatc cggctttgat gaagcaactg gccaccgagc tggccgaggg ctccttcaac
     7921 cgggtcacgg tcgatggcga cacctccacc aacgactcgt ttgtggtgat tgccaccaac
     7981 cgcgcagccc atcctcccat cgactcgctg gccagcccgg ctggtcaggc cctgaaggcg
     8041 gccatgctgg gtgtcgcacg caatctggcg caggcgattg tgcgcgacgg cgagggtgct
     8101 accaaattca tcagtattga ggtggctggc gggcaatcgc aagccgagtg ccgccaggtg
     8161 gcctatgcga ttgcgcattc accgctggtg aaaactgctt tttttgccag cgaccccaat
     8221 cttggccgga tactggcggc agtgggctat gccggtatcg cagacctgga tcagacccgc
     8281 atagatctgt atctggacga cgtgctggtg gttgtgcagg gtggacggaa cccggcttac
     8341 cgtgaagaag acggccagcg cgtcatgcag caaagcgaga tcacggtacg ggtactgctg
     8401 gggcgcggta gtgcgacgga tacggtgtgg acctgcgatt tgagccatga ctatgtgtcg
     8461 atcaacgccg actaccgttc atgaacccga gttttgagca tttgctggcg cgtgccgagc
     8521 agctcatcgc ccgcatcgag gccgtgctgc cgcagccgct tgccgcacct gattggaccg
     8581 cagcccacgc ctaccgctac cgtaagcgta gcgccgggcg cagcagcctg gagccggtgc
     8641 gccacattgg cgtgatgaaa ctcagtgacc tgaaagaaat tggggcgcaa aaggaaaaga
     8701 tccagcgcaa caccttgcag tttgtgcgcg gtgacctggc caacaacgtg ttgctgaccg
     8761 gtgcccgtgg caccggcaag tccagcctga tccgcgcctg cctgaacgaa tacgcgcatc
     8821 tgggcctgcg cctgatcgaa gtggacaagg ccgatctggt cgatttgccc gacatcgttg
     8881 acctggtgtc agaccggccc gagaaattca tcgtgttttg tgacgacctg agctttgagg
     8941 acggcgagcc tggctacaag gcgctcaagt cgattctgga cggctcggtt gccgccacca
     9001 cgcccaacgt gctgatttac gccaccagca accgccgcca cctgctgccc gaacacatga
     9061 aggacaacct cagctacacc cacagcccgg acggtgaggt tcatccgggg gaggtggtgg
     9121 aagaaaaaat atcgctctcg gaacgttttg gcctgtgggt cagcttttac tcattcaacc
     9181 aagacgagta cctggccatc gtggcgcagt ggctcagctc gtttggggtc gccgccagcg
     9241 tgatcgagca tgcccgcgca gaagccctcg tctgggcgct cgagcgtggc gcgcgcagtg
     9301 gccgcgtggc ctaccagttt gcccgcgact ttgccgggcg ccagtcggct gcacccggtg
     9361 cagctcagcc atgaccgagc cacgtaaacc tctggtcgcc gacgggcacg aagcccgtgc
     9421 aggcgggcca gtccggccgg tgctcgatgt cgctgtaggt gtgctgatta ggtccaacgg
     9481 tgattttttg ctgacctcgc gccctgcagg caaggtgtac accggctact gggagtttcc
     9541 cggtggcaaa ctggaggcgg gcgaaacggt ggagcaagcc ctggcgcgtg aattgcaaga
     9601 agagctgggg attcaaaccg gccagatcgt gcgttggcaa agcgagctgg tggactaccc
     9661 gcacgcattg gtgcgtctgc atttctgtaa agtgtttgat tggacgggtc aactgcag
//
LOCUS       DAOSLL010000100         9714 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6768_length_9714_cov_5.276633, whole genome shotgun sequence.
ACCESSION   DAOSLL010000100 DAOSLL010000000
VERSION     DAOSLL010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9714
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6768_length_9714_cov_5.276633"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="PLB25_06125"
     CDS             <1..747
                     /locus_tag="PLB25_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOE41190.1"
                     /translation="MRIGVALIDILTGIYATSAILAALEVRHRTGEGQHIDMALLDVG
                     MAVLANQAAGFLNTGQVPQRQGNTHPSLAPYQTFATADGSMLLAIGNDGQFARFCEAA
                     GHPEWAQDARFRSNSLRVNNREALIPLLQAITGTRSTAGWIDLLEGKAVPCGPINNLA
                     QAFADDQVIARGLIIKQPVEHTDKALPATDSIASSRTVQTVASPLRLTKTPPVVCRPP
                     PALGQHTEEVLAELGLDATAIAALRGRGVI"
     gene            926..1468
                     /locus_tag="PLB25_06130"
     CDS             926..1468
                     /locus_tag="PLB25_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOE41191.1"
                     /translation="MPRYGAVAISLHWLLALAILALFGLGVYMQDLPFSPWRLKLYNW
                     HKWAGISFLLLSLLRLVWRITHQPPALPAAITQAMPAWQSLVHHATHHLLYALFFIVP
                     LLGWAYSSAAGFPIVWFGQIALPDFLPADKALADLIKPLHQLSAFALMGLAALHVAAA
                     LKHQWIDRDGLLLRMLPGRS"
     gene            1500..2072
                     /locus_tag="PLB25_06135"
     CDS             1500..2072
                     /locus_tag="PLB25_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOE41192.1"
                     /translation="MKNIPLLSILTTALLAGASVPAAAQQKLLPAQSELVFVAKQMGV
                     PITGHFKKFDAQVSFDAAKLATSKVTFAVDMGSGTLGAPEMDSELPKAPWFNAPKFPQ
                     ATFASSAFKVLGAGKYEVAGQLNIKGQTVQVLVPLVMTQNGAVTVATGVLPIKRLAFK
                     IGDGEWADTSMVADDVQVKFKFSLTGVGKL"
     gene            2131..2706
                     /locus_tag="PLB25_06140"
     CDS             2131..2706
                     /locus_tag="PLB25_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HOE41193.1"
                     /translation="MRSTLLPLAAAVALLAGTVSAQAASYAIDPAHTYVSFEISHFGT
                     STNRGRFDKKEGSIEFDRAGKSGKVDIVVDTTSINTGFAAFNKHLQSADLFNAEKFPT
                     MKFVADKFTFNGDKVTEVAGTLTLLGKTGPLNLKATNFNCYDSPMLKREVCGGDFEAM
                     LDRTQFGMNYGIDWGFPKIVRLVVQVEAVKQ"
     gene            complement(2736..3290)
                     /gene="hemG"
                     /locus_tag="PLB25_06145"
     CDS             complement(2736..3290)
                     /gene="hemG"
                     /locus_tag="PLB25_06145"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HOE41194.1"
                     /translation="MAHHLLLAYSTTDGHTRHICERLQFVMTALGQRVTLVPIEQADA
                     LNLNQFERIVIGASIRYGHHQPQVAQFIARHQALLQSRPSAFFSVNIVARKPDKNRPD
                     NNPYLLKFLQQISWQPQLLGVFAGKLNYPGYRWFDRQMIRLIMLITHGPTDPKTVVEF
                     TDWAQVEAFAHAVCALPDATNAPA"
     gene            complement(3355..5409)
                     /gene="fusA"
                     /locus_tag="PLB25_06150"
     CDS             complement(3355..5409)
                     /gene="fusA"
                     /locus_tag="PLB25_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HOE41195.1"
                     /translation="MSLNMSEKIRTVALVGHGAVGKTTLVESLLAATGAIASRGSVEK
                     GNTVCDFDPVEKQLGFSLQSSLVSLSTADTLIHLIDTPGYPDFAGQAIGALAAVDTAL
                     IVISAQTGIELTAERMFALASERGLCRMIVINKIDADNIDLGRLVDDIRDRFGKQCLL
                     LDLPAHHAEEVVELLGHADGESDFASVAAAHRALIDQLIEEDDDLMARYLEDGTDPSP
                     QELHAPFVKALRAGHLIPILFVSAKTGAGVPQLLDALVKLAPNPAEGNPQPFYKGELG
                     QDPEPFAAQPDDALHVLAHVFKVIVDPFIGKVGVFRVHQGTIRKEAQLFAGSSKRPFK
                     VAHLYRLQGKEYVEVPLLVPGDIGAVAKVDEIDFDCVLHDSHDEDNIHLKPLAFPQPM
                     QGLAVQTKRKGDEQRLFEILHKLELEDPCFTVERHPGTNETVIRGLGDMHLRAKMLRM
                     QQQYKLELDTSVPQIAYRETIIGKAEGHCRHKKQSGGAGQFGEVMLRVEPLARGAGFE
                     FVDIVKGGAIPGVFMAAVEKGVQQALAEGAVGGFPLHDLRVIVHDGKTHAVDGKEVAF
                     VAAARKATLQAVRAATPIVLEPMVNIEVVAPEAAIGDLTGDLASKRGHITGTQPRPAA
                     TVAISGQMPLAELGDYQSRLKSLTGGQGSYSLAFSHYAQVGAEMQNKLAAAFKPDNLA
                     DD"
     gene            complement(5521..6435)
                     /locus_tag="PLB25_06155"
     CDS             complement(5521..6435)
                     /locus_tag="PLB25_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOE41196.1"
                     /translation="MSHVVLRKDHLDGLAVGLLIACSAFWGFQQVLVKATIVDIPPVF
                     QAAIRFVLATLVLGLWCRWRGVALFRRDGSLKAGLLAGALFALEFSCIYIGMQFSAAS
                     RLTVFLYTAPFWVAVLLPLSVKSERLRKLQWLGLALAFVAVLFALRDGMDQAGNPLQW
                     LGDLLALGAGLAWALTTVTIRTTRLAIVSPEKLLFFQIGVSAVTLPLVSLLLGEPWHW
                     QFSGFAITSLLLQTVVGAFASYLAWMWMLGRYPATKISVFAFLTPVFALLFGALWLSE
                     PVTPALLASLGLVAAGIVLVNRKATSRG"
     gene            complement(6509..7027)
                     /locus_tag="PLB25_06160"
     CDS             complement(6509..7027)
                     /locus_tag="PLB25_06160"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HOE41197.1"
                     /translation="MSPSPARKRAQVPRFSGTEFRTALGMFATGVTIVTARSPDGQLV
                     GLTANSFNSVSLKPPLVLWSLSQAASALDAFSAGSHYAINILAADQQALAQRFATRCV
                     DRFADVAFTQGSSGAPLLDGSVASFECFNRSRYVEGDHVIFVGEVEHCSHRAGASPLL
                     FHGGRFYTEHPL"
     gene            complement(7515..7985)
                     /locus_tag="PLB25_06165"
     CDS             complement(7515..7985)
                     /locus_tag="PLB25_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOE41198.1"
                     /translation="MHAALLQAEFFFETTKEIEAVKLTRDTQAGGYELLTAAELKKMI
                     DEGKPMVLVDTMPYEASYKKEHIAGAKQFLFPIPRMSTWDTKETDGKTEADFAALLGP
                     DKDKAVVIYCGFVKCTRSDNGAVWARKLGYTHVYRFPGGLFAWKGADFPLEQVK"
     gene            7980..8828
                     /locus_tag="PLB25_06170"
     CDS             7980..8828
                     /locus_tag="PLB25_06170"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOE41199.1"
                     /translation="MQLARTTLARNEQLAAAGFVAPAQVQTKQEELIDADGRLQSLER
                     ARLALQHDLQALQGERSALSAQLQTDLSQIERSRSSLEQEMSENAARQSTVVTAPFDG
                     TVTALNLKLGQSVQSGQTLVTLVPVSGGTPGSGPQQLAVQAPLQAQLFAPSRTAGFVR
                     PGQTVYLRYDAYPYQKFGLHTGHITSVSATPFAPSELPANLAQQLVAQSGSNEALYRI
                     NVQLDEQSIQAYGEPVALKAGLTLEADVLQKRRKVWEWVLEPVLAARQQMKVLGAVSK
                     AVSAGI"
     gene            9389..9694
                     /locus_tag="PLB25_06175"
     CDS             9389..9694
                     /locus_tag="PLB25_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41200.1"
                     /translation="MQKLFNIFHPQDLNWKSLSVFCLQTSLMTSLGAMGIVLLVRSFA
                     LILGVNDVHAIPVESRIGYKYFIFMVFFSPIIETFILGLLIKLATILVTIPRQSRGL"
BASE COUNT         2061 a         2869 c         2817 g         1967 t
ORIGIN      
        1 atgcgcatcg gggtggcgct gatcgacatc ctgaccggca tttatgccac cagcgcgata
       61 ctggcggcgc ttgaagtgcg ccacaggacg ggcgagggcc aacacatcga catggcgctg
      121 cttgatgtcg gcatggcggt gctggccaat caggccgcag gcttcctgaa caccgggcag
      181 gtgccacaac gccagggcaa cacccacccc agcctggctc cttaccagac ctttgccacg
      241 gccgacggat caatgctgct ggcgattggc aacgatggcc agtttgcccg cttttgtgag
      301 gccgcaggcc acccagaatg ggcgcaggac gcgcggtttc gcagcaacag cttgcgggtg
      361 aacaaccgcg aagcgctgat cccgctgctg caggccatca ccggcacccg cagcaccgcc
      421 gggtggatag acctgctcga gggcaaggcc gtgccttgcg gcccgatcaa taacctggcg
      481 caggcttttg ccgatgacca ggtcatagcc cgtggcttga taataaaaca gcctgtagag
      541 catacagata aagcgctgcc agctactgac tcaatagcat caagcagaac ggtgcagacc
      601 gttgccagcc cgcttcgcct gaccaaaaca ccgccggttg tgtgtcggcc accgccagcc
      661 ctgggccaac ataccgaaga agttctggct gaactgggac ttgatgcaac agccattgca
      721 gccttgcgcg gccgtggggt catttagcct ggttccatgt cgctttgcca tgcattgaaa
      781 tggtttgaac tttgtcatca attcaattga atatgtttgc actgactgat ctctagactt
      841 gcggctcttg cttcagggta atccctgatc aacccctttt tggagaaccg tcgtgacctc
      901 atccctgtca gattcatcag cgcacatgcc gcgctacggc gccgtggcca tcagcttgca
      961 ctggctgctg gcactcgcca tcttggccct gtttgggctg ggtgtctaca tgcaggacct
     1021 gccgttttca ccctggcggc tcaagctcta taactggcac aaatgggccg gcatcagctt
     1081 cttgctgttg tcgctgttgc gtctggtctg gcgcatcacg caccagccac cagcactgcc
     1141 cgccgccatc acccaggcca tgccggcctg gcaaagcctg gtccatcacg ccacccatca
     1201 cctgctgtac gcgctgtttt tcatcgtgcc gctattgggc tgggcgtaca gctcggcggc
     1261 aggttttccg attgtctggt ttggccagat tgcgctgcct gactttttgc ccgccgacaa
     1321 ggccttggcc gacctgatca agccactgca tcaactgtct gccttcgcct tgatgggctt
     1381 ggcggctctg catgtggcag cagcactcaa acaccaatgg attgaccggg acggcctgct
     1441 gctgcgcatg ctgcctggcc gcagctaacc gatcaaacac gacagaaacg atacgcatga
     1501 tgaaaaacat tccacttctt tcgatcctga caacggcctt gctggctggt gccagcgtac
     1561 ccgccgccgc ccagcaaaag ctgctgcccg cgcaaagcga actggtcttt gttgccaagc
     1621 aaatgggggt gccgatcacc ggccacttca agaagtttga tgcccaggtc agttttgatg
     1681 ccgccaaact ggccaccagc aaggtgactt ttgccgtgga catgggcagc ggcacgctgg
     1741 gcgcacccga gatggacagc gagttaccca aagccccgtg gttcaacgcg cccaagttcc
     1801 cgcaagccac ctttgcttcg tcggccttca aggtgcttgg cgctggcaaa tacgaagtag
     1861 ccggtcagct caacatcaag ggccagaccg ttcaggtgct ggtgccgctg gtgatgacgc
     1921 aaaacggagc cgtcacggtg gccaccggtg tgctgccgat caagcgactg gccttcaaga
     1981 ttggtgacgg cgaatgggcc gacacctcga tggtggccga cgacgtgcag gtcaagttca
     2041 agttttccct gaccggggtg ggcaagctct aacgcccccg gatctttctc aaccgtgttt
     2101 ctttcaatca aacctttcca ggagtttttc atgcgttcaa ccttgttacc tctcgctgcc
     2161 gccgtcgcct tgctggcggg cactgtgtct gcacaggccg ccagctacgc cattgacccg
     2221 gcgcacacct atgtgagctt tgagatcagc cactttggca ccagcaccaa ccgtggtcgc
     2281 tttgacaaga aagagggcag cattgagttt gatcgggccg gcaaatcagg caaggttgac
     2341 atcgtcgtgg acaccacctc aatcaacacc ggctttgctg cgttcaacaa gcatctgcaa
     2401 agcgcagacc tgttcaatgc cgaaaaattt ccaaccatga aatttgtcgc tgacaagttc
     2461 actttcaatg gcgacaaggt gacagaggtt gcaggcaccc tgacgctgct gggcaaaacc
     2521 ggccctttga acctcaaagc caccaacttc aactgctacg acagcccgat gctcaagcgc
     2581 gaagtctgcg gcggcgactt tgaagcgatg ttggaccgta cccagtttgg catgaactac
     2641 ggcattgact ggggattccc gaaaatcgtg cgcctggtgg tgcaggtgga agcggtcaaa
     2701 caataagccg gttcaagccg cgccgatgcg gttcttcagg ccggtgcgtt ggtggcgtcc
     2761 ggcagcgcgc acaccgcatg cgcaaaggcc tccacctgcg cccagtcggt gaactccacc
     2821 actgtcttgg ggtcggttgg cccatgcgtg atcagcatga tcaggcggat catctggcgg
     2881 tcaaaccagc ggtaaccggg gtaattgagc ttgccagcaa acacccccag caactgcggc
     2941 tgccaactga tctgctgaag gaacttcagc aggtacgggt tgttgtcggg acggtttttg
     3001 tcgggcttgc gtgccacgat gttgacgctg aaaaacgcgc tgggccgact ctgcagaagc
     3061 gcctggtgtc tggcaatgaa ctgcgccacc tgcggctgat ggtggccata gcggatgctg
     3121 gccccgatga cgatgcgctc gaactggttc aggttaagtg catcggcctg ctcgattggc
     3181 accagggtca cccgctggcc cagcgccgtc atgacaaact gcagccgctc acaaatatgc
     3241 cgggtgtggc cgtcggtggt ggagtaggca agcagaaggt gatgtgccat ggcgcagcct
     3301 ggtcaattga tgttggggct gagctgcgcc agcgctgatt tttgcccagg ccgatcagtc
     3361 atccgccagg ttatcgggct tgaaggcagc agccagcttg ttttgcatct cggcaccaac
     3421 ctgtgcgtaa tgcgaaaacg ccaggctgta agagccctga ccgccggtga gtgacttcag
     3481 gcggctctgg tagtcgccaa gctcagccag cggcatttgc ccgctgatgg caacggttgc
     3541 tgccgggcgt ggctgggtgc cggtgatgtg gccacgtttg gatgccaggt cgccggtcag
     3601 gtcgccaatg gcagcctcgg gtgccaccac ttcgatgttg accattggct ccagcacgat
     3661 cggggtggcg gcccgtaccg cttgcagcgt ggccttgcgg gctgcggcca caaaggcgac
     3721 ttccttgccg tcaaccgcat gggtcttgcc gtcatgcacg atgacgcgca agtcgtgcaa
     3781 tggaaacccg ccaaccgcac cctctgccaa ggcctgttgc acgccctttt caaccgccgc
     3841 catgaagaca ccgggaatcg cgccgccctt gacgatgtca acaaattcaa aaccggcacc
     3901 acgcgccagc ggttcaaccc gcagcatgac ctcgccaaac tggccagctc cgccgctttg
     3961 ctttttgtgg cggcaatggc cctcggcctt gccgatgatg gtttcgcgat aggcgatttg
     4021 cggcacgctg gtgtctagtt ccagcttgta ctgttgctgc atgcgcagca tcttggcacg
     4081 caggtgcatg tcgcccaggc cgcgaatcac cgtctcgttg gtacccggat ggcgctcgac
     4141 ggtaaagcag gggtcttcga gttcgagctt gtgcaaaatt tcgaacagcc gctgctcgtc
     4201 gcccttgcgt ttggtctgca ctgccaatcc ctgcatcggc tgggggaacg ccagcggctt
     4261 gaggtggatg ttgtcctcgt cgtgcgagtc atgcagcacg cagtcgaaat cgatttcgtc
     4321 caccttggcc acggcaccaa tgtcgccggg caccagcagc ggcacttcaa catattcctt
     4381 gccttgcagg cggtacaggt gagccacctt gaacgggcgc ttggaggacc cggcaaacaa
     4441 ctgggcctct ttgcgaatgg tgccctggtg cacccggaac acgccgacct tgccaatgaa
     4501 cgggtccacg atcaccttga aaacatgcgc cagcacatgc agcgcgtcat ctggctgggc
     4561 ggcaaaaggc tcagggtctt ggccaagctc gcccttgtaa aacggttgcg ggttgccctc
     4621 tgccggattg ggtgccagct tgaccagcgc atcgagcagt tgcggcacgc cagcgccagt
     4681 cttggctgac acaaacaaaa tcggaatcaa atgcccggca cgcagtgcct tgacaaacgg
     4741 cgcgtgcagt tcttgcgggc tggggtcggt gccgtcttcc aggtaacgcg ccatcaggtc
     4801 gtcgtcttct tcaatcaact ggtcgatcag cgcgcggtgt gccgcagcaa ccgaggcaaa
     4861 gtcactttcg ccgtcggcat ggcccagaag ttcgaccact tcttcggcat ggtgcgcagg
     4921 caggtccagc aacaggcatt gtttgccaaa acggtcgcgg atgtcgtcca ccaggcggcc
     4981 caggtcgatg ttgtcggcat caatcttgtt gatcacaatc atgcggcaca ggccgcgttc
     5041 gctggccagt gcgaacatgc gctcagcggt gagttcaatg ccggtttggg cgctgatgac
     5101 gatcagggcc gtatcgactg ccgccagcgc gccgattgcc tgaccggcga aatcggggta
     5161 accaggggtg tcaatcaggt gaatcagggt gtctgccgtg ctcaggctga ccagcgacga
     5221 ctgcaatgag aaaccaagtt gcttttcaac cgggtcaaag tcgcacacgg tgttgccttt
     5281 ttcgaccgag ccacggctgg caatggcgcc agtggcggcc agcaggcttt cgaccaaggt
     5341 tgttttaccc acggcaccat gaccgaccag ggccacagtg cggatttttt cggacatgtt
     5401 caggctcata aaggtctcat ttttgtgttg atgtagagcc aagtttacgc cgttgccgac
     5461 gctttttttc agccacacct tgccgctcgg cagtgctttg tgtgcacctt cagatgggca
     5521 tcaaccccgg cttgtcgcct tgcggttaac cagcacaatg cctgcagcca ccagacccag
     5581 gctagccagc aaagcagggg tgactggctc gctcagccaa agtgcaccaa acagcagcgc
     5641 aaacaccggt gtcaggaacg caaacaccga aatcttggtg gccgggtagc gacccagcat
     5701 ccacatccag gccaggtagc tggcaaacgc gcccaccacg gtttgcagca acagcgaggt
     5761 gatggcaaag ccgctgaact gccagtgcca cggctcgccc agcagcagcg acaccagcgg
     5821 cagggtgaca gcgctcacgc caatctgaaa aaacagcagc ttttccggcg aaacaatggc
     5881 cagccgggtg gtgcggatgg tcaccgtggt cagggcccag gccagccccg cgcccagcgc
     5941 cagtaagtcg cccagccact gcagcgggtt acccgcctga tccatgccgt cgcgcagtgc
     6001 aaacagcacc gccacaaagg ccagcgccag gcccagccac tgcagcttgc gcagccgctc
     6061 ggacttgacc gacagcggca gtaacaccgc cacccagaat ggcgcggtgt acaggaagac
     6121 agtcaggcgc gaggcggcag aaaactgcat gccgatgtag atgcaggaaa attccaacgc
     6181 aaacaaggcc ccggccagca atcctgcctt gagcgagccg tcacgccgaa acagcgcgac
     6241 accgcgccag cggcaccaca ggcccagcac aagggttgcc aatacaaagc ggatggcggc
     6301 ctgaaaaact ggcggtatgt caacaatggt ggccttcacc agcacttgct gaaagcccca
     6361 gaatgcgctg caggcgatca gcagtccgac ggccaggcca tcgagatggt cttttcgcag
     6421 cacgacatgg ctcattggca cagggctttt aggtggcggg cgcagccaca ggggcgcatt
     6481 gtttgcggca ccaaacaagg ggcgctcttc acagcggatg ctcggtgtaa aacctgccgc
     6541 catgaaagag cagcggtgat gccccggcgc ggtggctgca atgctcaacc tcaccaacaa
     6601 aaatcacatg atcgccctcg acatagcgac tgcggttgaa gcattcaaaa ctggcaacgc
     6661 taccgtcaag cagcggtgcg ccgctgctgc cctgggtaaa cgccacatcg gcaaaacgat
     6721 caacacacct ggtggcaaag cgctgcgcca gcgcctgctg gtcggctgcc aggatgttga
     6781 tggcatagtg cgagccagca ctgaacgcat ccaacgcgct ggccgcctgc gacaggctcc
     6841 acagcaccaa cggcggcttg agagagactg agttaaacga attggcggtc aggccaacca
     6901 actggccatc aggcgaacgc gcggtgacga tggtcacacc ggtggcaaac atccccaggg
     6961 cggtgcggaa ttcggtgccg gagaaacggg ggacttgggc gcgcttgcgt gctggggacg
     7021 gtgacattgg caggaattta cccgaaattt ctgccaatct ggctgacccg ccgcaaagta
     7081 gccccgccaa cgatggtgct tcaagccatc agatgcgtcg gaatagctca atcttgctgc
     7141 agttagctat ttaatcagta gctgccagcg cttatttgac gggggctata ggcacatttc
     7201 attcaaattg tcaggcacag attgcgatcc tgacccctgc cgctgccgcc gtcggcaacg
     7261 ctgcgcaccg agcgcgcagc aaaaagcggc cagccccaaa atcagagctg tcgtgcgtca
     7321 aaaccgcttc aggcatccaa cacccggttt gactgtcgag atgcgcagat gggtgactgg
     7381 gcgcaaatcg ggccagaccg ggacctagca gcgcaaccgg cagccgacct tgaggccgac
     7441 agacaagtca gttggtgata ggtcgagacg gcgactttga tgcgctggcg gtccatcaga
     7501 tgcgcctttt caggctattt cacttgttcg agcggaaaat cagcgccctt ccatgcgaac
     7561 aggccgccag gaaaacgata cacgtgggta tagccaagtt tgcgcgccca gactgcgccg
     7621 ttatcactgc gtgtgcattt gacgaaacca caatagatca ccaccgcttt gtctttgtcc
     7681 gggccaagca aggcagcaaa atcagcctcg gtttttccat cggtttcctt cgtatcccag
     7741 gtactcatcc ggggtatcgg aaaaagaaac tgtttcgccc ccgcaatgtg ttccttcttg
     7801 tagctggcct cgtagggcat ggtatcgacc agcaccatgg gcttgccctc gtcaatcatt
     7861 tttttcagtt cggccgcagt cagcagttca tagccgccgg cctgggtatc gcgggtcagc
     7921 ttgacggctt caatttcctt ggtcgtttcg aagaagaact cagcctgcaa cagcgccgcg
     7981 tgcaactggc ccgcaccacc ctggcgcgca acgaacaact ggccgcagca ggctttgtgg
     8041 caccggccca agtgcaaacc aagcaagaag aactgattga tgccgacggc agattacaaa
     8101 gtctggagcg cgcccgcttg gccctgcagc atgacctgca agcattgcag ggcgaacgca
     8161 gtgcgctgag cgcccaactc cagaccgacc tgagccagat cgaacgcagc cgctccagcc
     8221 tggagcagga aatgagtgaa aacgccgcgc gccaaagcac cgtggtcacc gccccgtttg
     8281 acggcaccgt gaccgcgctg aacctcaagc tgggccagtc tgtgcaaagt ggccaaaccc
     8341 tggtcacgtt ggtgcctgtg tccggcggca caccggggtc agggccacag cagcttgccg
     8401 tgcaagcccc cttgcaggcg caactgtttg cccccagccg caccgctggg tttgtgcgcc
     8461 ccggccaaac cgtctatttg cgttacgacg cttaccccta ccaaaaattt ggcctgcaca
     8521 ccggccacat caccagtgtg tcagccacgc cctttgcacc cagtgagttg cctgccaacc
     8581 tggcccaaca gttggtcgcc cagtcgggca gcaatgaggc gctgtaccgc atcaacgtgc
     8641 aactcgacga gcaaagcatc caggcctatg gcgagcccgt ggccttgaaa gccgggctga
     8701 cgctggaggc tgatgtgttg cagaagcgaa ggaaagtatg ggagtgggtg ctggagccgg
     8761 tgctggcggc acggcaacaa atgaaggttc tgggtgcagt gtctaaggca gtttccgcag
     8821 ggatctgact tggggccgat acccgaagct gaaagtccga cagcgttttt cggatggttt
     8881 aatggagtga atgatgagag agcttaatgt ttgcgaagtg ggtgcggtgg ctggtgggag
     8941 gaatgttgaa ctttggggag caatagctcg cgaaatcggt atcggcctcg ctatcgaggg
     9001 cattgctaac gccatttcat atggctggaa ctggtggaat accccgacac caacatacgg
     9061 caacggaaac ggtgccaccg ggcctggtta cagcggggcg ggtataggcg gcaacgtgtc
     9121 acccggtagc agtggttcaa gtcactttgg ataaagcgcg agttcaaatt caacgtgcgg
     9181 ctttatgaac aacggcttgg gtgggcgaga tgtgggagca tccaaaccac tcgacctcca
     9241 agtcaagttt gcgtcgatgg caatcgatgc cgtcgtgtca atcaagttgg taagtcaatg
     9301 cgttgattga attcactgga ttcgaagccc aattgaacga ctcacgcatc catgttgtgg
     9361 gttgttttca ttgatcatta tttttcaaat gcaaaaatta tttaatatat tccatccgca
     9421 agatttaaat tggaaatcgc tttctgtttt ttgtctgcaa acttctttga tgacttcact
     9481 gggggcgatg ggtattgttt tattggttcg aagttttgca ttgatattag gggtaaatga
     9541 tgtccacgca attccagtgg aaagtaggat tggctataaa tattttattt ttatggtatt
     9601 cttttcgccc attattgaga cattcatttt gggcttgcta ataaaattag caacaatatt
     9661 agtaaccatc ccccggcaaa gccgggggct ttaagaattg tgagccgcta aaag
//
LOCUS       DAOSLL010000101         9669 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6800_length_9669_cov_4.859268, whole genome shotgun sequence.
ACCESSION   DAOSLL010000101 DAOSLL010000000
VERSION     DAOSLL010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9669)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9669)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9669
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6800_length_9669_cov_4.859268"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(262..1515)
                     /locus_tag="PLB25_06180"
     CDS             complement(262..1515)
                     /locus_tag="PLB25_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41201.1"
                     /translation="MNLVPINIDAIIIGHPLPCPLRDEGGVLLANQGFMVHSRQELEV
                     MVGRRSQIYIDASQSDNFRRGYVNRLNNLVLADRALGQIAEVQVSPYDGKRSVAAEVS
                     DEPEWLDLQSQAHAMLRDTRGESFLPRLERLQNELERHTLRNPDGTLFAVIHLAGNEI
                     NHYSATHAMLVCVICSLAARDILKWPPAHVSAMCSAALSMNIGMTELQDRLAVQKSPP
                     TQEQLSRIDGHAAGSVELLQKMGVSNELWLQAVLNHRGNAPGPLAARSDGQRIARLIQ
                     RADMFAARLSPRASRAPESPSTAMQSCYFDENRQIDEAGVAIIKAVGIYSPGTFVKLV
                     TNEIAVVIKRGLNTTMPRVAVLINRHGMVTGEPILRDTSLAEFRIVASVPHKDVKVNH
                     NLDRLLALTRQMPTERSWSGMKRSN"
     gene            1604..2125
                     /locus_tag="PLB25_06185"
     CDS             1604..2125
                     /locus_tag="PLB25_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HOE41202.1"
                     /translation="MPENTSFPSFANGPGVPGLTQKLCRPVDLLTRLTHLPRPLVFTN
                     GVFDVLHRGHVMYLEQARTLGASLLVALNTDQSARRLGKGPDRPLNNEMDRACVVAAL
                     ASSCCVTWFDEDTPLELINLVRPDILVKGGDYDMDKLAETAAMRSWGGKALALPFVTG
                     YSTTALVRRIRNS"
     gene            complement(2166..2501)
                     /locus_tag="PLB25_06190"
     CDS             complement(2166..2501)
                     /locus_tag="PLB25_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41203.1"
                     /translation="MPSILAPRCQLLKCAPLCRGLQAAVAAVAAVSFSACLDGQLVMA
                     YLQTLKRALPAGCALWLGGQGCVDLSNDLRQGCAVFVDTPSAVLGWQQMGSAALASIC
                     MMTTLVSKL"
     gene            complement(2699..2869)
                     /locus_tag="PLB25_06195"
     CDS             complement(2699..2869)
                     /locus_tag="PLB25_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41204.1"
                     /translation="MPDTLGLPQKVQSLLTKLSLVTDAGALTQWFESLLAQSGLSLVC
                     GESAASVQPDRR"
     gene            complement(3060..3305)
                     /locus_tag="PLB25_06200"
     CDS             complement(3060..3305)
                     /locus_tag="PLB25_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOE41205.1"
                     /translation="MICVKSAPLTLPAIEELTGLSREVLRKWELRSHFHQPARGERGE
                     RRYSFADAHRLQLVGQLIACGMRPGRLVPLECQANIT"
     gene            3416..6364
                     /locus_tag="PLB25_06205"
     CDS             3416..6364
                     /locus_tag="PLB25_06205"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOE41206.1"
                     /translation="MSSEQYSAPAVAAEINESSPQIVAIGASAGGLEALEKLFDGLSS
                     QSGATFVVIQHLSADHKSMMANLLARHTAMPVVMVEQDMPIEANRVYLIPPGSIMHMG
                     AGKLTLTPKNPRTLTLPIDVFFKSMAEHYGARSVGVVLSGTGSDGTRGAAAINDAGGF
                     LVAQEPEDAKFDGMPRSVIATGLVDAILPVEQIGARLLAHINNQPIVKAAATLDTVNN
                     PMRATSPEAALSGIMHLLLQVGGIDFQEYKTGTVMRRIERRMNVRQVTALETYLELLN
                     NDRNEVLTLRRELLIPVTSFFRDADAFEILSRQIIEPLVAKKQAGESIRVWLAAVSTG
                     EEAYSVAMLFLEAFDQLKHWPTLKIFATDVEQQNIETAGAGTYPESIAAEVSSQQLER
                     FFVKKGNTFVVKNELRQCIVFARHNLLTDPPFTRMDLVTCRNALIYFRPAAQERALKR
                     LQSALLPGAHLFLGSSESLGDLQKDFTTVSARHKMWEMVRPLSFPLEVTKGTGYGYPG
                     IPLTSSQRLRQGRIKPTMVEQGFSALIKAFSPPAAILVNGRHELVHSYGEVNRFLQLR
                     EGHASLDMNRILPEPLVPVAVALVFKAGREGSSVVSDRLKFTNPEGETEHLRLSAWPV
                     DESDGDRLTLLAFERLNAIAQPNEALTTIDLDSETAERMEVLERELTATRESLQATVE
                     ELETSNEELQSTNEELMSSNEELQSSNEELQSVNEELNTVNAEYQEKIDILNRLNADL
                     DNMAQAVAAGTVFVDEMLNLTRFSPDATHIFKLRESDIGRPLDDLAHTLLYPELIADL
                     ARTLRDEARVEKEIRGINGLHYFVRMLPYRVPSSSNRGAVITFMDVTALHEVTRLQSI
                     IDALAEHIAVLNSSGVITLINSAWRRFATDNGDPNLVHSGPGVNYLEVCRDFHEPPDP
                     SASAAQRGVREVLEGRSRQFSLEYPCHSTTEDRWFVMHVSAVGGEQPGAVVSHVNITQ
                     WRNQSRLASS"
     gene            6361..9000
                     /locus_tag="PLB25_06210"
     CDS             6361..9000
                     /locus_tag="PLB25_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE41207.1"
                     /translation="MTLLPNRTNSQKTERLAVLKQRAQAVLNLHEGAAPEGPSTALNA
                     AELLEDLRIYQVELEMQNDELRAAQQEADLLHRRYQSLFELMPLPALVVDGNGAVDDC
                     NERASALLGRGNHNVNPDNRFWHVVHKGARARLHAALRDVRSGESMLLQQVQIGKADS
                     RSPVFDIHLIGLSMDYKLDRRLLLLLVDRTAEAARVADQRFFSLLLDSSDSLIYASDR
                     QGKMLLANQTLLHFLGLDRAAVHGHARVDFLPLRDAILQSESDQRVLQNGKAMTLEEK
                     MHGHAEGPVDFLTRKFPLRDLEGQIYGVGGISTDITFFKSQQKQNLLSETVFMAAQEA
                     IIVTDADARIVRVNPAFSRQSGFSSEVVMGRNPRILKSGRHDADFYKTMWSSIAFQGS
                     WSGEVCNRRADGTEYNVLSNVNAVRESDGKVLYYFAVQTDVTLLHQTQLRLAHQASYD
                     ALTELPNRSLFNDRAAQLLASSKRHGNAFAVLFVDLDRFKEVNDTLGHHVGDALLRSV
                     ARRLQEGVRAEDTVARVGGDEFAVLLPASDHAGAQAVAGNLLARLREPLPLEAAQHYR
                     PMASIGMAIFPQDGDTPDLLLRNADMAMYGAKISGRNRWASYTPQMSQANTRAFSIQT
                     ELAEAIAQQQLRVHFQPKCQLGTGQLVGAEALVRWERPDHGLIMPGEFIRVAEKTGLL
                     TSLDRWVMEDALRQLGLWLRSGRWRSGWRLAINQSVADLQQPEMPRHLKALLQVHQVP
                     PEALELEITEDALLQQTPTQLACLSELRDMGISLAIDDFGTGYSSLAYLRQLPISVIK
                     IDQSFISTMLVNKNDAVLVHAIIDLGHNLGRTLVAEGIELAEQSARLRELGCELGQGY
                     LFGRPVSAEAFEQQWLALDEQLS"
     gene            9019..>9669
                     /gene="lpxC"
                     /locus_tag="PLB25_06215"
     CDS             9019..>9669
                     /gene="lpxC"
                     /locus_tag="PLB25_06215"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HOE41208.1"
                     /translation="MIKQRTIKTLTRAVGVGLHSGQRVEITLRPAQPDTGIVFRRIDL
                     PQPVDIAVSALTVTDTRMASTISNGNAKVHTVEHLMSACAGLGVDNLHVDITAEEVPI
                     LDGSAASFVFLLQTAGIELQNAPRRFIRLLKAVEVRQGEGATEKWARLEPYHGFQLSF
                     EIDFSHPAVDSTGQRVLFDMSTGSYSKDIARARTFGFTKDVEMLRANGLALGGGMDN"
BASE COUNT         2018 a         2814 c         2854 g         1983 t
ORIGIN      
        1 gatttcgcat gttaagtcga gccaatcaac acgccatcgg ccattgtgcc gctgggctga
       61 cttcagtcgg gctggtcagt gcaagccgag cctgggagcg cttcagactc atcgatcaag
      121 cagcaagaag cgaccccggc gaggggtact ggattggcag acccacaacc cggttgtggc
      181 acaaaaagcc ggtgccattg ggccgctgcg gtcgattcgc agccaaggca acaggttcgc
      241 caggttcctt gaacaccggc atcagttgct ccttttcatg cccgaccaag atcgctcggt
      301 gggcatttgc cgggtcagag ccagcaagcg gtctagattg tggttgacct tgacatcttt
      361 gtgcggcacg cttgcaacaa tgcgaaattc agccagactg gtgtcgcgca aaatgggctc
      421 gccggtgacc atgccatgcc ggttgatcag caccgccact ctgggcatgg tggtgttgag
      481 cccgcgcttg atcaccaccg cgatctcgtt ggtgaccagt ttgacgaagg tgccgggcga
      541 gtaaatgcca acggccttga tgattgccac accggcctca tcaatctgcc ggttctcgtc
      601 aaaatagcag gactgcatcg ccgtgctggg cgactccggg gcacgtgagg cacggggtga
      661 caggcgtgcc gcgaacatgt cagcgcgctg gatcaaccgg gcaatgcgct gaccatcact
      721 tcgtgccgcc aacggcccgg gggcattgcc ccggtggttg agcaccgcct gcaaccaaag
      781 ttcgttggaa acccccatct tctggagcag ttcaactgat ccggccgcat ggccgtcaat
      841 gcggctgagc tgctcctggg ttggcgggct cttctgaacg gccagacgat cttgcaactc
      901 ggtcatgccg atgttcatgg acaacgcagc actgcacatt gcactgacat gcgcaggtgg
      961 ccatttgagg atgtcgcgcg cggccaggct gcaaatcacg cagaccaaca tggcgtgggt
     1021 ggcgctgtag tgattgattt cattgccggc caggtgaatg acggcaaaaa gagtgccatc
     1081 cgggttacgc aaggtgtggc gttcgagttc gttctgcaag cgctcgagtc gtggcaaaaa
     1141 tgattcaccc cgggtgtcgc gcagcatggc gtgtgcctgc gactgcaaat caagccattc
     1201 gggttcatca ctgacctcgg ctgccacact cctttttccg tcatagggcg aaacctgtac
     1261 ttctgcaatt tgcccgagcg cccggtctgc cagcaccaga ttgttcaatc ggttgacgta
     1321 gccacggcgg aagttatctg actggctcgc atcaatgtag atctggctgc gccgcccgac
     1381 catcacctca agttcttgtc ggctatgcac catgaaaccc tgattggcca gcaaaacgcc
     1441 gccttcgtca cgcagcgggc agggcagtgg atgaccaatg atgatggcat caatgttgat
     1501 gggaaccaga ttcatagtga aggatgatta tgccaagttg cctgtccgac ggctgtcggt
     1561 ctgtcagcat aagcacttgc gcaggcctga tagcatccaa cacatgcctg aaaacacctc
     1621 ttttccttcc tttgccaacg gccctggcgt acctggcctg acgcaaaagc tatgccgacc
     1681 ggtggatttg ttgacgcgac tgacacattt gccgcgtccc ctggtgttca ccaatggcgt
     1741 ttttgacgtg ttgcaccgtg gccatgtcat gtacctggag caggcccgga cgctcggcgc
     1801 tagcctgctg gttgcactca ataccgatca atcagctcgc cggctcggca aggggcctga
     1861 tcgcccgctg aacaatgaga tggaccgtgc gtgcgtggtg gcagcgctgg ccagcagttg
     1921 ttgcgtaaca tggtttgatg aagacacacc gcttgaactg atcaacctgg tacgcccgga
     1981 catactggtc aagggcggcg actacgacat ggacaaactg gccgagacgg ctgccatgcg
     2041 gtcgtggggc gggaaggcgc tggccttgcc tttcgtcacc ggttactcga ccaccgcgct
     2101 cgtcaggcgg atccggaatt catgatgaaa tgatgctcta ggccttacac cgcttgcgca
     2161 agcagctata actttgatac caatgtagtc atcatgcaaa tcgatgcaag ggcggcactg
     2221 cccatttgtt gccagcccag cactgcgctt ggggtatcga caaacaccgc acagccttgc
     2281 cgcaaatcgt ttgacaggtc cacgcaaccc tggccaccca gccatagcgc gcaaccggct
     2341 ggcaaggccc gttttaaagt ctgcaggtag gccataacca gttggccatc caggcacgca
     2401 ctgaaggaaa ctgccgccac tgccgccact gccgcctgca gacccctgca cagcggcgcg
     2461 cacttcagca actggcatct cggtgccaag attgatggca tcggccccac gcaaccgcaa
     2521 tcgggcatgc agcgcaagca ggcccagact gtgcaattcg gtcggtggtg tggtcagcaa
     2581 tatccggggc aatgcggctt tgcccgctgc cgtcggcacg gcagatgtga ccacttgccg
     2641 caaggtttcg gtgtacagat gttcggcatg cactccgagc cgtcccgact gccaggcatc
     2701 acctacggtc tggttgaacg ctggcagcag attcgccaca aaccaggctc agtccggact
     2761 gagcaagcag gctttcaaac cattgtgtca gggcacctgc atcggtcacc aggctcaact
     2821 ttgtcagcag cgactgcacc ttctgcggca ggcccaatgt atctggcacg ggcttctcca
     2881 cactgaccct ggccaccagc acggcctgca gttgggcggc actcaggggt caagcaagaa
     2941 agatttggac aaatgaggtc gtgatggcaa gtgccgggct agacgcaagc cgcgcggtgt
     3001 agctggctac acaagcggtt tgcaacaacg cattgcgctt gtcagtgcgg ccgaactgtt
     3061 caagttatgt ttgcttgaca ctctagcggc accaaacgac cagggcgcat gccgcaggcg
     3121 atcaactgcc ccaccagttg cagcctgtgg gcatcagcaa agctgtaacg ccgttcaccg
     3181 cgctcgccgc gcgcaggctg gtgaaaatgg gagcgaagct cccatttgcg caacacttca
     3241 cgcgacagcc cggtaagttc ctcaatcgct ggcagcgtca aaggcgcgga tttgacacag
     3301 atcatggaat actgtttttg tccgggacaa aatattgagc accgataatg cggcaacaag
     3361 tcggtggaca aaatacatcg atcaatgggt tttagttgga acctgaaggt aaaccatgtc
     3421 atctgaacaa tactcagcgc cagcagtggc ggccgaaata aacgaatcga gcccgcaaat
     3481 cgtcgcgatt ggtgcgtctg ccggggggct ggaggcgctg gaaaaactgt tcgatggatt
     3541 gtccagccag tccggtgcca cttttgtggt catccagcat ttgtcggcag accacaaaag
     3601 catgatggcc aacctgctgg cacgccacac cgccatgccg gttgtgatgg ttgagcagga
     3661 catgccgatc gaggcgaacc gggtttatct gattccgccg ggctcaatca tgcacatggg
     3721 ggcgggcaag ctgaccttga cgcccaaaaa cccgcgcacc ctgaccctgc caatcgatgt
     3781 cttcttcaaa tcgatggccg agcactatgg cgcacggtca gtcggcgtgg tgctttcggg
     3841 taccggatca gacggtaccc ggggcgctgc ggccatcaac gacgccggtg gtttcctggt
     3901 cgcgcaggaa cctgaagatg ccaagtttga cggcatgccg cgcagcgtga ttgcgactgg
     3961 cctggtcgat gctattttgc cggtggagca aattggtgcg cgcttactgg cccacatcaa
     4021 caaccagccc atcgtcaagg cggcggcaac gctcgacacc gtcaacaatc cgatgcgggc
     4081 aaccagccct gaagccgcct tgagcggcat catgcacctg ctgctgcaag tcggtggcat
     4141 tgattttcag gaatacaaaa ccggcaccgt gatgcggcgc attgagcggc gcatgaatgt
     4201 gcgccaggtt actgcccttg agacttacct ggaactgctc aacaatgatc gaaacgaggt
     4261 cttgacgctg cgccgcgagc tgctgatccc ggtgaccagc tttttccgcg atgccgatgc
     4321 ttttgagatt ttgtcgcgcc agatcattga gccactggtg gccaaaaagc aggcgggcga
     4381 atcgatccgc gtctggctcg ccgcagtgtc gaccggcgaa gaggcgtatt ccgtcgccat
     4441 gttgttcctg gaggccttcg accagctcaa gcattggcca accctgaaga tttttgccac
     4501 ggatgtcgag cagcagaaca ttgagacggc cggtgccggg acctaccccg agtcgattgc
     4561 cgccgaggta tcgtcacagc agcttgagcg tttctttgtc aagaaaggca acacctttgt
     4621 ggtcaagaac gagttgcgcc agtgcatcgt gtttgcccgc cacaatctgc tgaccgaccc
     4681 accgtttacc cgcatggacc tggtgacctg ccgcaatgcg ctgatttact tcaggccggc
     4741 cgctcaggaa cgcgcactca agcggcttca atccgccttg ctgcccggtg cccacctgtt
     4801 tctgggttcc agcgagtcgc tcggtgatct gcagaaagac ttcaccaccg tcagcgcccg
     4861 ccacaaaatg tgggaaatgg tccgtccctt gagttttccg ctggaagtca ccaagggcac
     4921 tggctacggc tatccgggca tcccgttgac cagcagccag cgcctgcgcc agggccgcat
     4981 caagccgacc atggttgagc agggtttttc agcgctgatc aaggcgtttt caccacctgc
     5041 ggccatattg gtcaatggtc ggcacgaact ggtgcactct tacggcgagg tcaaccgctt
     5101 cttgcaattg cgtgaaggcc atgccagtct ggacatgaac cgcatcctgc ccgagccgct
     5161 ggtgccagtg gctgtcgcat tggtgttcaa ggccggtcgt gaaggttcca gcgtggtgtc
     5221 ggaccgcctg aaattcacca accccgaggg tgaaaccgaa cacctgcgcc tgagcgcctg
     5281 gccggtcgat gagtccgatg gtgaccgcct caccttgctg gcttttgagc gtctaaacgc
     5341 cattgcacaa cccaacgagg cactgaccac cattgacctc gactcggaaa ccgccgagcg
     5401 catggaggtg ctggagcggg aactcaccgc cacccgcgaa agtttgcagg ccaccgtcga
     5461 agagctggag acatccaacg aggagttgca gtctaccaac gaggagctga tgtcctcgaa
     5521 cgaagagttg caaagctcca acgaggaact gcaatcggtc aacgaagaac tcaacaccgt
     5581 caacgccgag taccaggaaa agatcgacat cctgaaccgt ctcaacgctg acctcgacaa
     5641 catggctcag gcagttgcgg ctggcaccgt gtttgtcgat gagatgttga acctgacgcg
     5701 cttttcgccc gatgccacgc acatcttcaa gctgcgcgaa tccgacattg ggcgaccgct
     5761 tgatgacctg gcgcatacct tgctgtaccc tgagctgatt gcggatctgg cgcgcaccct
     5821 gagggatgaa gccagggttg aaaaggaaat ccgtggcatc aacggcctgc actattttgt
     5881 tcgcatgttg ccgtaccggg tgccgtcctc gtccaaccgg ggggctgtga tcaccttcat
     5941 ggatgtcacc gcgctgcatg aggtgacgcg cctgcagtcc atcattgatg ccctggccga
     6001 acacatcgcg gtgctcaaca gcagtggtgt catcaccctg atcaacagtg cctggcggcg
     6061 ttttgcgacc gacaacggcg accccaacct ggtgcactca ggcccggggg tcaattacct
     6121 ggaagtttgc cgagacttcc atgagccgcc tgacccgagc gccagcgcag cgcagcgtgg
     6181 tgtgcgcgag gtgctggagg gccgcagcag gcagttttca ctggaatatc cttgccattc
     6241 gactaccgag gatcgttggt ttgtcatgca tgtctcggcc gtcgggggcg aacagcctgg
     6301 tgccgtggtg agccatgtca acatcaccca atggcgtaat caaagcaggc tggcttcatc
     6361 atgacactcc taccgaacag aaccaactcc cagaaaacag aacgtctcgc ggtgctcaag
     6421 cagcgcgctc aggcggtgtt gaacctgcat gagggggccg cacccgaagg gcccagcacg
     6481 gctttgaatg cagccgaatt gctggaggac ctgcgcattt accaagttga actggagatg
     6541 cagaacgatg agctgcgcgc cgcgcagcaa gaggctgacc tgctgcaccg gcgctaccaa
     6601 tcactgtttg agttgatgcc gctgccagcc ctggtggttg atggcaacgg cgcagtagat
     6661 gattgcaacg agcgtgccag tgccctgctt gggcggggca accacaatgt caacccggac
     6721 aaccgctttt ggcatgtcgt ccacaaaggt gcccgcgccc gcttgcatgc ggccctgcgt
     6781 gatgtgcgct caggtgagtc catgttgttg cagcaggtgc agattggcaa ggccgatagt
     6841 cgcagtccgg tgtttgatat ccatctgatc ggtttgtcga tggactacaa gcttgatcgt
     6901 cggttgttgt tgctgctggt tgatcgcact gcggaagctg cgcgcgtggc ggatcagcgg
     6961 tttttctcct tgctgctgga ctccagtgac agcctgattt atgcctctga ccggcaaggc
     7021 aagatgctac tggcaaacca gacattgctg cattttctgg gtttggatcg ggctgctgtt
     7081 cacggccacg cgcgagttga ctttttgcca ttgcgcgatg ccatattgca gtccgagtcc
     7141 gaccagcggg tgctgcaaaa tggcaaggcc atgacgctgg aagaaaaaat gcacgggcac
     7201 gccgagggtc cggttgattt tctgacgcgc aaattcccgc tgcgcgatct ggaagggcaa
     7261 atctatggag tcggtggcat ttcgaccgac atcaccttct tcaaaagcca gcaaaagcag
     7321 aacctgttga gtgaaaccgt gttcatggcc gcgcaagaag cgatcatcgt caccgatgcc
     7381 gatgcgcgca ttgtgcgggt caacccggct ttttcccggc agtccggctt ctcatcagag
     7441 gtggtaatgg ggcgcaaccc gcgtatcctg aagtcgggtc ggcatgatgc tgatttttac
     7501 aagacgatgt ggtcgtccat agcctttcag ggcagttggt caggtgaggt gtgcaatcgg
     7561 cgcgctgacg gcaccgagta caacgtcctg agcaacgtca atgcggtacg tgaaagcgat
     7621 ggcaaggtgc tctactactt tgcagttcag accgatgtca ccctgttgca tcaaacacag
     7681 ttgcgtctgg cccatcaggc ttcttatgat gccttgaccg aattacccaa ccgctcgctg
     7741 ttcaacgacc gggcagcaca gttgctggcc agttcaaagc ggcatggcaa cgcatttgcg
     7801 gtgctgtttg tcgatctgga ccgtttcaaa gaggtcaacg atacgctggg tcatcatgtc
     7861 ggtgacgcgc tgctgcgtag cgttgcccgg cggttgcagg aaggggtccg cgccgaagac
     7921 acggtggcgc gtgtcggtgg cgacgagttt gctgtgctgc tgccggcatc cgatcatgct
     7981 ggcgcgcagg ctgtagcagg caacctgctg gcccggctgc gtgaaccttt accccttgag
     8041 gcggcccagc attaccgccc gatggccagc attggcatgg ccatttttcc gcaagatggc
     8101 gacacccctg atctgctgct gcgcaacgca gacatggcca tgtacggtgc caagatcagt
     8161 ggccgcaacc gatgggccag ctacacgcca caaatgagtc aggccaatac ccgggcgttt
     8221 tccatccaga ccgagctggc cgaggcgatt gcgcagcaac agttgcgagt ccattttcag
     8281 cccaaatgcc agcttggcac tggacaattg gtgggagctg aggcgctggt gcgatgggag
     8341 cggcccgacc atggactgat catgccgggt gaattcatcc gggttgcaga aaagaccggc
     8401 ttgctgactt cgcttgaccg atgggtcatg gaagatgccc tgcgtcagtt gggactttgg
     8461 ctgcgcagcg gccgctggcg cagcgggtgg cgactggcca tcaaccaaag tgtggccgac
     8521 ctgcagcaac cggaaatgcc acggcacttg aaggcattgc tgcaagtcca ccaggtgcca
     8581 cccgaagcct tggagcttga aatcaccgaa gatgccctgc tgcagcaaac cccgactcaa
     8641 ttggcatgcc tgagcgagtt gcgcgacatg ggaatttcgc tggcgattga tgactttgga
     8701 acgggttact ccagtctggc ctacttgcgc cagctcccca tctcggtgat caagattgac
     8761 cagagcttca tcagcaccat gctggtgaac aagaatgatg ccgtgttggt gcacgcaatc
     8821 attgacctcg ggcacaatct gggccgcacc ctggtggcag aaggcattga actggcagag
     8881 caatccgcgc ggctgcgcga actcggctgc gaactgggtc agggttacct gtttggcagg
     8941 ccagtgtctg ccgaggcgtt cgaacagcaa tggctggccc tggacgagca gctttcctag
     9001 ctttattgga caaaccgcgt gatcaaacaa agaacgatca aaaccctgac ccgcgccgtc
     9061 ggtgtcggct tacacagtgg gcagcgggtg gaaatcaccc tgcgcccggc ccagccagat
     9121 accggcattg tgtttcgccg gattgatctg ccacagccgg tcgacattgc cgtgtcagcc
     9181 ttgactgtga ctgacacccg catggcatcg actatttcca acggcaatgc caaggtgcac
     9241 acggtcgagc acctgatgtc ggcttgcgcc ggactgggtg tggataacct gcatgtcgat
     9301 atcaccgccg aagaagtgcc gattctggac ggctcggcgg catcgtttgt tttcttgctc
     9361 caaaccgccg gcattgaact gcaaaacgcg ccgcgccgtt tcatcaggct gctcaaagcg
     9421 gtggaagtgc gccaaggcga aggagcgacc gaaaaatggg cacgtctgga gccgtatcac
     9481 gggtttcagc tcagcttcga gattgacttc agtcacccgg cggtggactc gaccgggcag
     9541 cgcgtgctgt ttgacatgag caccggttcg tactccaaag acattgcccg ggcccgaact
     9601 ttcggcttta ccaaagatgt tgaaatgctg cgtgccaatg gtctggcgct cggtggtggc
     9661 atggacaat
//
LOCUS       DAOSLL010000102         9657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6810_length_9657_cov_5.242449, whole genome shotgun sequence.
ACCESSION   DAOSLL010000102 DAOSLL010000000
VERSION     DAOSLL010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9657
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6810_length_9657_cov_5.242449"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="PLB25_06220"
     CDS             complement(<1..731)
                     /locus_tag="PLB25_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOE41209.1"
                     /translation="MKLTIAQRLSLGFLLIVSTFLVLTAFAAWQIGIVSKATSSMVTQ
                     TQLLQLAGKWQANVKQSSARALASAYGDGTAILDFFKESQEAVSRETTELQKVFLTLV
                     QDDGGKKAADQVGEVRKSWLAVRSEISALKAAGDETAAKALVQSKFVAVTDDYIRATQ
                     VLLDDQISDVAETHEHIHNMFHQLYLVSGVLLLLCIAIAVYVSWSLSRGIASGIGSAR
                     LAAQRIGAGDLSQPLPHTGQDEIGQL"
     gene            881..1114
                     /locus_tag="PLB25_06225"
     CDS             881..1114
                     /locus_tag="PLB25_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41210.1"
                     /translation="MQPGQHASQQRHARGFSAIFHLHSLFNVATPMEVNFVSWPLMMN
                     SMCRHFSPGSAMATKARANQLGIINIQQLNESN"
     gene            complement(1594..1983)
                     /locus_tag="PLB25_06230"
                     /pseudo
     CDS             complement(1594..1983)
                     /locus_tag="PLB25_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016834481.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            2552..2809
                     /locus_tag="PLB25_06235"
     CDS             2552..2809
                     /locus_tag="PLB25_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006479978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HOE41211.1"
                     /translation="MQTISATDLARNTREILDKVAIRGESVIIERNHAMIAQIIPPPQ
                     TMTASQALAELMLPMLAPAQASAWLRDSKGDFGDGVRDPWA"
     gene            2800..3222
                     /locus_tag="PLB25_06240"
     CDS             2800..3222
                     /locus_tag="PLB25_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOE41212.1"
                     /translation="MGMIFDSCIWLGLAANQLDRQVVIDAAGDAPVFTSTISLGELNF
                     GVQSCTDPTERAMRAAYLRQVESRPAIGVSKHTAAAFGVLAAAVKQSGRSPRPRYNDL
                     WIAAQAIENGYVLLTLNRNDFVDLPGLRLAVLSPKVQA"
     gene            complement(3282..4570)
                     /locus_tag="PLB25_06245"
                     /pseudo
     CDS             complement(3282..4570)
                     /locus_tag="PLB25_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875300.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(4582..4785)
                     /locus_tag="PLB25_06250"
     CDS             complement(4582..4785)
                     /locus_tag="PLB25_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41213.1"
                     /translation="MIARAINQSGLNLQSLVADVFEQATGVEVAGQEIAAATRDLANR
                     TEQTAANLQQTAARWGRRPNMDR"
     gene            complement(4782..5126)
                     /locus_tag="PLB25_06255"
     CDS             complement(4782..5126)
                     /locus_tag="PLB25_06255"
                     /inference="COORDINATES: protein motif:HMM:NF020036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOE41214.1"
                     /translation="MVRHPHMPTEAFADMWRSLKEGQSWRALVKNRRKDGDHYWVCAN
                     AAPMRRNAQVVGYLSVRTPPARAEVDAAEALYRRFKEGRAVDLAFHRGLSKSPVAKRG
                     STSTSIAATKSV"
     gene            complement(5148..5288)
                     /locus_tag="PLB25_06260"
     CDS             complement(5148..5288)
                     /locus_tag="PLB25_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41215.1"
                     /translation="MRTNLPVTQRECHLSANETLLSTTDTSSHISYANAAFIRVSGFS
                     KE"
     gene            complement(5267..5686)
                     /locus_tag="PLB25_06265"
     CDS             complement(5267..5686)
                     /locus_tag="PLB25_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE41216.1"
                     /translation="MPIELLIVDDSDLVRAGLRAALGSVAGTAVIRAASTLTEALASA
                     RRDPPTLVILNLNLPDALGIDIIQQIKQRSPTLWLAVLAIHAEPGYRRRCLALGADWF
                     FDKTTETGALLEVVRQLVMQNPIINLNQGIPCEPICQ"
     gene            complement(5875..8682)
                     /locus_tag="PLB25_06270"
     CDS             complement(5875..8682)
                     /locus_tag="PLB25_06270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE41217.1"
                     /translation="MSTKSGYRVMVVDDRPENIYLMQAILSPAGYEVVVTTSGEAALR
                     LAQASAPDLILLDVLMPEMDGYAVCSRLKQMEVTRQIPVIFVTAVDDTEAEARSFALG
                     AADYITKPVKLSRVLARVKTHIALYRQQQSLQGMFHDVIELAPDAMLLSDAQGQIVRI
                     NAQAEQLFGYTRQELVGQAIEVLLPKHLREKHPVLRDGFTTAPMARRMSSDVQARRKD
                     GSNCEVAVTLNQIQTADDALIVSAFRDIGWRKAAETDLRIAAAAFESQDGVVVTDLDD
                     MILRSNQAFSRISGYSAEEALGRKMNFLKSDRHGDDFYRAMWQAVIQDGVWHGEIWNR
                     HKSGEVHPHWLTISAVKDAQGQVTHYVGTYTDYTERHAAEEQLRIAAVAFESQECMMV
                     TDANKVILRVNRAFADVTGFSPEELVGQTPRMLQSGRHDEDFYRDMTESIDRTGGWQG
                     EIWDRHKNGEVFPKWLSISAVKDAGGAVTHFVSAQHDISERKKTEATINQLAFFDPLT
                     SLPNRTLLLDRLKQAITASVRSQHYGALLFIDLDHFKDLNDSLGHDVGDLLLKQVAQR
                     LSFCVREGDTVARLGGDEFVVLLVNLSTSATEAAIDAEVVTRKIIATLDQVYRFGGSP
                     HHSTASIGLTFFQGAQVSLDELLKQADLSMYKAKADGRNTYRFFDPAMEVAVKKRSAL
                     EADLRWAVEAQQLYLHYQPQVTQDGQVGHVTGSEVLLRWRHPQRGMVSPADFIDLAEE
                     TGLILPLGHWVLESACHQLAVWANVPALAHLTVAVNVSALQFAQADFVEQVMAVVQRT
                     GANPLRLKLELTESLLVGNIADIIEKMVSLKSRGIDFSLDDFGTGYSSLSYLSRLPLN
                     QLKIDRSFVSDVLINPDDAAIAKTIIALAHSLRLGVIAEGVETDAQREFLTGAGCHAY
                     QGYLFSRPLPVAEFEAFVRSH"
     gene            complement(8679..8897)
                     /locus_tag="PLB25_06275"
     CDS             complement(8679..8897)
                     /locus_tag="PLB25_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41218.1"
                     /translation="MNQQHAQHPSQPATLWDAVDALNGLRDALVELALELHDLQFELE
                     DDRYQAARERALLYVANARSGNAPRRES"
     gene            complement(8936..>9657)
                     /locus_tag="PLB25_06280"
     CDS             complement(8936..>9657)
                     /locus_tag="PLB25_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE41219.1"
                     /translation="VTMRGINEASGKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVASEVRALASRSAEAAKEIKTLINASVERVAQGTSQVDQAGTTMQEVVAAIK
                     RVTDIMAEISAASSEQSLGVSQVGAAVTEMDQVTQQNAALVEQMAAAASSLMSQANQL
                     VETVAVFKLSAEDAVPHGVAPSPVASRSSGTQNQPFNGREKRFASGPKAAAVTAPAMA
                     LAPVRKPSAQAAQAEGDWTSF"
BASE COUNT         1938 a         2849 c         2803 g         2067 t
ORIGIN      
        1 agttggccga tttcatcctg cccggtgtgg ggtaatggct ggctcaggtc acccgcgcca
       61 atgcgttggg cggcaagccg tgcgctgcca atgccactgg caatgccccg cgacaggctc
      121 caactgacgt acacggcaat ggcgatgcac aagagcaaaa gaacaccact caccaggtag
      181 agctggtgga acatgttatg gatatgctca tgtgtctcgg caacgtcgct gatctggtca
      241 tccagcagaa cctgggtggc gcgaatatag tcatcggtaa ccgcgacaaa cttgctttgt
      301 accagtgctt tggctgcagt ctcatctccg gcagccttca atgcactgat ttcggaacga
      361 actgccagcc aggacttgcg gacttcaccc acctggtcgg cggctttttt tccaccgtcg
      421 tcctgtacca gagtaaggaa aactttttgc agttcagtgg tttcgcgcga cacggcttcc
      481 tgagattcct tgaaaaagtc caatatggcg gtaccgtcgc cataagcgct ggctagagcc
      541 cgagccgagc tttgcttgac attggcctgc catttgccgg ccaactgcag caattgcgtc
      601 tgggtgacca tgcttgaagt tgccttggaa acaattccga tctgccaggc agcgaaggcc
      661 gtgagcacca ggaaggttga cacaatgagc aggaagccca ggctcaatcg ttgggcgatc
      721 gttaatttca tggtgttttt caatcgttgg ttacagggac gcggcagcct tgtgtttgcc
      781 gtgcaaggga ctcaagcggt gctcattggc gaaaaaagct gtgtcatgaa cacaagtttt
      841 ttcagtgata gacgcgattc gactataaat ttctctcagc ttgcaacctg gccagcacgc
      901 cagtcaacaa cgtcatgcgc gtggcttttc tgcaattttt cacctgcatt cccttttcaa
      961 tgtagcaact ccgatggaag tcaactttgt gtcgtggccg ctcatgatga attcgatgtg
     1021 ccgccacttc agcccaggct ccgcaatggc gaccaaggct cgcgcgaatc agcttggaat
     1081 catcaacatt cagcaactga atgagagcaa ctgaatgagt gttggttatc ttgcctgctc
     1141 agacccgttt gcgctatcgg gcaatgctga tttggctgta gggcattgct gagcgggggg
     1201 tcgggattgc cctacccatg gttgctgtcg tcagggtcac ttgatcgact ggctattgct
     1261 gcaagaccat tctgacgaag gctggggctg cagcatactg ctctggcgct ggcctgatcg
     1321 agcgtccggc gcttcaacgg gctggctcaa gacagttttc ggacttttat acgatttggg
     1381 tagcgcctca ataagtcgtg gcaaacccgt cattcccctg aaggcgggaa tgacggaatc
     1441 gtttcatttt gcgagtgtcg ccacggatta ttgagaagtt accgatttag gcctccagca
     1501 ggcgtggaat aagcgctaga tgctattcat tagatagtga atcgaagtat tcccgcttga
     1561 cctctaagag gcggccgact ggcacgatac ccactaatga ctgcggacaa acgcctcaaa
     1621 ctctgccacc ggcagcggtc ggccaaacaa atacccctga taagcgtggc agctagcgtc
     1681 cgccagaaac tcccgctgcg catcggtttc aacaccttcg gcaataaccc ccaggcgcaa
     1741 actgtgtgcc agggcgatga tggttttggc aatcgccgca tcgtccgggt tggtcagcac
     1801 atcgttgact aaagacttgt cgatcttcaa ctgatccagc ggcaggcggc tcaggtaccc
     1861 cagcgacgaa tggccggtgc caaaatcatc gagcgagaaa tcaatccgcc ggcctttcag
     1921 actgaccatc ttttcgatga tgtccgagat gttgcccacc agcaggcttt cgcccagcgc
     1981 cacaaagccc agcagcagca agctgtgctg ggcactgtag aagcctttgg gcaggcttaa
     2041 gtgtttgccc acgccactga gcaatccgtt ggcgcacagc gctggtaagc ctgtgagcag
     2101 gcccgccatg ggcacatcgc agacgttttc atagcgatac ccttgccgcg ccggatgcca
     2161 gtgaagccag atccctcggt tgtccacttg tcttctccaa cgagtgttta tggctattgg
     2221 cttttccagc ttcgagcaaa catggattgc ttcgctgcgc tcgcaatgac tgggggcgac
     2281 gcaatgactg ggtggggtca tggcatgaaa aggccttctt cattgcgagg cgcgcagcga
     2341 cgcggcaatc cagagggaac aaatcggtat ccagtgcctc ggctgagtca caagtcacta
     2401 tgaattacat agctgtcagc gcaagtgatg caagggctac aggcactttt tgctcgcaaa
     2461 tgctacattt caagacctga ctctgttgca tcagttaaat acataaaaaa tgtatactac
     2521 gcattgctac attcaacctg gagatggagc catgcaaacc attagcgcaa ccgacctggc
     2581 cagaaacacc cgcgagattc tggacaaggt tgccatccgc ggtgagtccg tgatcatcga
     2641 gcgcaatcac gccatgattg cccaaatcat tccgccgccg caaaccatga ccgcatccca
     2701 ggcactggct gaactgatgt tgcccatgct ggcacccgcg caagcaagcg cttggctcag
     2761 ggacagcaaa ggcgactttg gcgatggagt gcgtgatcca tgggcatgat tttcgacagt
     2821 tgcatctggt tgggtcttgc cgccaaccag ttggaccgtc aagtggtcat cgacgctgcc
     2881 ggggacgcgc cggtgtttac ctccacaatt tctttgggtg agctgaattt tggcgtgcag
     2941 tcatgcaccg acccgaccga gcgggcgatg cgtgcagcct acctgcgcca agtggaaagt
     3001 cgcccagcaa taggggtgtc caaacacacg gctgccgctt ttggggtgct ggcagcggct
     3061 gtcaaacaaa gcggccgctc accccggccg cgttacaacg acctttggat agccgcgcaa
     3121 gccatcgaaa atggatatgt gcttttgaca ctcaacagga atgattttgt ggacctgccg
     3181 ggactgcggc tggcagtact ttcacccaaa gtgcaggcct gatcactggt gactgattaa
     3241 tgctacgttt caagacttga ccctatcgac cgcgatgcca gctaatgact gcaggcaaac
     3301 gcttcaaact ccgccaccgg cagcggtcgg ctaagcaaat acccctgata agcgtggcag
     3361 cccgcgtccg ccagaaattc ccgctgcgca tgggtttcaa cctcctcggc aataaccccc
     3421 aggcgcagac tgtgcgtcag ggcaatgatg gttctggcaa tggcggcatc gtccgggttg
     3481 gtcagcacat cgttgacaaa agacttgtcg atcttcaact gatccagcgg caggctgctc
     3541 aggtacgcca gcgacgaata gccggtgcca aaatcatcca gcactgcctg gccgttacgg
     3601 catggcgcag gcctttttcc atggcaacac gtttgacgac gatggttgcc atgcctgggt
     3661 caaagaagcg catggcgtta cgccccgcgt ccttggcccg gtgcatggca aggtcagcct
     3721 gcttcatcag gtcgtccatg ctggcgcgcg agccctggac cagcgtggtt ccgatgctgg
     3781 cactgctgtt gtgcgacaac tcgcctaact ggcatggctg gctgagtgtg gcgaggattt
     3841 tcgtggccac ggcttcggtg gccgtggccg cgtcaacctc gctggcgctc aggccagcca
     3901 gaatcaccac aaactcgtcg cccccgagcc gcgccacggt atcgcccgca cgcatgcagg
     3961 actgcaggcg ctgcgccacc tgcttgagca acaggtcgcc aatgtggtgg ccacgggtgt
     4021 cgttgagggt cttgaagtgg tccaggtcga taaacagcag ggccccaaaa ctgccactgc
     4081 gcgcaccggc cgtcatggcc tgcgtgagcc gatcgagcag cagggtgcgg ttgggcaggc
     4141 cggtgagctg gtcaaaaaac gccagctcgc ggattctcgc ggtggccttt ttgcgttcgg
     4201 tgctgtcttg atgggtgccg acgtagtggg tcacgacacc gtcgtcggtc ttgacggcag
     4261 aaatggtcag ccacttggga tagatctcgc catttttgcg ccggtcccag atttcgccct
     4321 gccaaccgcc ggtgcgtttg atggtgcgcc acctgctgcg ataaaacgcc ttgccatggc
     4381 ggcctgactg cagcatgtgc ggggtttggc cgacgatgtc cccggcggcg tagcccgtcg
     4441 atgcaacaaa ggcccgattg acccgcagca ccacaccctt gcggttggca accaacatcg
     4501 gttcttctga ctcgaaggcg gtggcggcaa tgcgcagttg ctcctcggtc actttaagat
     4561 ggctgatgtc ggtatgcgag atcacctgtc catattgggc ctgcggcccc agcgagcggc
     4621 ggtctgctgc agattggcag ccgtttgttc ggtgcggttg gccaggtccc gggtggcggc
     4681 ggcaatctcc tggccggcca cttcgacacc cgtggcctgc tcaaacacat cggccaccag
     4741 cgactgcaag ttcagcccgg actggttgat ggcgcgtgca atcaaaccga tttcgtcgca
     4801 gcgattgagg ttgaggtact gccccgcttc gccactggcg acttgctcaa gccccgatga
     4861 aaggccaggt cgacagcgcg tccttccttg aaacggcggt agagtgcctc ggcggcatcg
     4921 acttcggcgc gcgcgggcgg ggtgcgcacc gacaaatagc ccaccacctg agcgttgcgg
     4981 cgcatcggcg cggcattggc acagacccag tagtggtcgc catctttgcg ccggtttttt
     5041 accagggcac gccacgactg accttccttg aggctgcgcc acatatcggc aaaggcctcg
     5101 gtcggcatgt gcgggtggcg caccatgttg tggggttgcc cggcgattta ttctttggag
     5161 aaacccgaaa cgcggatgaa agccgcgttg gcgtaggaaa tatggctgga ggtgtcggtg
     5221 gttgacagca gcgtttcgtt ggccgacagg tggcattccc gttgggtcac tggcagattg
     5281 gttcgcatgg gatgccttgg ttgaggttga tgatcgggtt ttgcatgacg agctggcgga
     5341 ccacttccag cagcgcaccg gtttcggtgg ttttgtcaaa aaaccaatcg gcaccaagtg
     5401 ccagacagcg ccggcggtaa cccggctcgg catggatcgc cagcacggca agccacaggg
     5461 tgggtgaacg ctgtttgatt tgttggatga tgtcaatgcc cagagcgtcg ggcaggttca
     5521 gattcagaat caccaacgtg gggggatcgc gccgtgcgct ggcgagcgcc tcggtcaggg
     5581 tagatgccgc gcggatgacc gcagtaccag ccacgctgcc aagcgccgca cgcaagcccg
     5641 cgcgaaccag gtcggaatca tcaacaatca gcagttcaat gggcatgccg cgatcttgcc
     5701 tgccccgacc cgcttgcgct atcgggcaat gctgatttga ctatagggca atgatgagtg
     5761 ttggtcagaa ttgccctacc catggttgct gtcgccaggt caccttgagc gactggctgc
     5821 tgctgcaaga ccaatctgac gaaggccagg acaccagcat gttgctcttg cccactaatg
     5881 actgcggaca aacgcttcaa actccgccac cggcagcggt cggctaaaca aatacccctg
     5941 ataagcgtgg caacccgcgc ccgtcagaaa ctcgcgctgc gcatcggttt caacaccttc
     6001 ggcaataacc cccaggcgca aactgtgtgc cagggcgatg atggttttgg caatcgcggc
     6061 atcgtccggg ttgatcagca catcgctgac gaacgacctg tcaatcttca actggttcag
     6121 cggcaggcgg ctcaggtacg acagcgacga atagccggtg ccaaaatcat cgagcgagaa
     6181 atcaatcccc cggctcttca aactgaccat cttttctatg atgtctgcga tgttgcccac
     6241 cagcaggctt tcggtcagtt cgagcttcag gcgcagcggg ttggcaccag tgcgctgcac
     6301 cacggccatc acctgttcga cgaagtctgc ctgggcaaat tgcagtgcgc tgacattgac
     6361 tgccactgtc aggtgcgcca gcgcaggcac attggcccat acagcaagtt ggtggcaggc
     6421 cgactcaagc acccaatggc ccagcggcag aatcagcccg gtttcttcgg ccaggtcaat
     6481 gaagtcggcg ggcgacacca tgccccgctg cggatgccgc cagcgcagca gtacttcaga
     6541 gccggtcaca tggccaacct ggccatcctg ggtgacctgg ggctggtaat gcagataaag
     6601 ctgctgtgcc tccactgccc agcgcagatc cgcttccagc gcggaacgct ttttgaccgc
     6661 tacttccatc gccgggtcaa aaaagcgata ggtattgcgc ccgtcggcct tggccttgta
     6721 catgctcagg tcggcctgtt tgagcagctc gtcaagcgac acctgcgcgc cctgaaaaaa
     6781 agtcaaacca atgctggcag tgctgtggtg agggctgccg ccaaaccggt agacctggtc
     6841 gagtgtggca atgattttcc tggtgaccac ttcggcatca atggccgcct cggtcgcgct
     6901 ggtgctcaaa ttgaccagta acaccacgaa ctcgtcaccc ccgaggcgtg ccacggtgtc
     6961 gccctcacgc acacaaaagc tcaggcgctg cgcgacttgc ttgagcagca aatcccccac
     7021 atcgtgaccc agcgagtcgt tcaggtcctt gaaatgatcg aggtcaatga acagcagcgc
     7081 gccgtaatgc tgacttctga ctgatgccgt gatcgcttgt tttagccggt ccagcaacag
     7141 cgtgcgattg ggcaggctgg tgagcgggtc aaaaaaggcc agctgattga tggtggcttc
     7201 ggttttcttg cgctcgctga tgtcatgctg ggcggagaca aaatgggtca cggcaccgcc
     7261 cgcgtctttg accgccgaga tgctcagcca cttgggaaaa acttcgccgt ttttgtgccg
     7321 gtcccaaatt tcgccctgcc agccaccagt gcggtcaatc gactctgtca tgtcgcggta
     7381 aaaatcttca tcgtgccgac ctgactgaag catcctcggt gtctgtccca ccagttcttc
     7441 cgggctgaaa ccggtgacgt cggcaaaggc ccgattgacg cgcagaatca ccttgttggc
     7501 atcggtcacc atcatgcatt cctgcgactc gaaggcgacc gctgcaatgc gcagttgctc
     7561 ctcggcggcg tgccgttcgg tatagtcggt gtaggtgcca acatagtggg taacctggcc
     7621 ctgcgcgtcc ttgacggccg agatggtcag ccagtgcgga tgcacctcac cagacttgtg
     7681 gcggttccag atttccccgt gccagacgcc gtcctgaatc accgcttgcc acatggcgcg
     7741 ataaaaatcg tcaccgtgac gatctgactt caggaagttc atcttgcgcc cgagcgcttc
     7801 ttcggcgctg tagccggaga ttctggaaaa tgcctggttg ctgcgcaaaa tcatgtcatc
     7861 taggtcggtg acgaccaccc catcttgcga ctcaaaggcg gcagcggcaa tgcgcaggtc
     7921 ggtctcggcg gctttgcgcc agccaatgtc gcgaaaggcg ctgacaatca aggcatcgtc
     7981 tgcggtctga atctggttca gggtcacggc cacttcacag ttgctgccat ccttgcgcct
     8041 tgcctgcaca tccgacgaca tgcgtcgtgc catcggcgct gtggtaaagc catcacgcaa
     8101 taccgggtgt ttttcgcgca ggtgcttggg caacaacacc tcaatagcct ggccgaccaa
     8161 ttcttgccgg gtgtagccaa acaactgctc ggcttgtgca ttgatgcgca cgatctggcc
     8221 ttgagcatcc gacagcagca tggcatcggg ggccagttcg atcacatcat ggaacatgcc
     8281 ctgcaggctt tgctgctgcc ggtacagcgc aatgtgggtc ttgacccttg ccagcacccg
     8341 cgacagtttg actggcttgg tgatgtagtc ggctgcaccc agcgcaaaac ttcgcgcctc
     8401 ggcctcagtg tcatcgaccg cagtgacaaa aatgaccggt atctgccggg ttacttccat
     8461 ctgcttgagg cggctgcaga cggcgtagcc atccatctcg ggcatcaata catccagcaa
     8521 gataaggtcc ggcgctgaag cctgggccag ccgcagcgcc gcttccccac tagtagtcac
     8581 cacaacctca tacccggccg ggctcagaat cgcctgcatc agatagatgt tctcgggccg
     8641 gtcatccacc accatcacgc ggtagcctga cttggtgctc atgactccct cctgggggca
     8701 ttgccgctac gggcgtttgc aacatacagc aaggcgcgtt cgcgggcggc ctggtacctg
     8761 tcatcctcca gttcaaactg caagtcgtgc agctccagcg cgagttcgac caaagcgtca
     8821 cgcaggccat tgagtgcatc gacggcatcc cacagggttg ctggctgcga tgggtgttga
     8881 gcgtgttgct ggttcatgtt cgggatgcgc ttgaatgatt gacccgtgtt acggtttaaa
     8941 agctggtcca gtcgccttcc gcctgggcag cttgggccga cggcttgcgc acaggtgcca
     9001 gggccatcgc cggggcggtg acagccgccg ctttcgggcc actggcgaag cgcttttcac
     9061 gaccgttaaa cggctggttc tgggtgcccg aggaacggct tgccactggg gaaggcgcca
     9121 ccccatgcgg cactgcgtcc tctgcggaca gcttgaacac cgccaccgtc tcgaccaact
     9181 ggttggcctg cgacatcagg ctgctggcgg cggctgccat ttgttccacc agcgccgcgt
     9241 tttgctgggt cacctggtcc atttccgtca ccgctgcacc gacctggctg acgcccagac
     9301 tttgctcgct gctggcggca ctgatttcag ccatgatgtc tgtgacacgt ttgatggcgg
     9361 ccaccacttc ctgcatggtg gtgccagctt gatcgacctg gctggtgccc tgtgccaccc
     9421 gctcgacact ggcgttgatc agggtcttga tttctttggc tgcttcagcg ctgcggctcg
     9481 ccagggcacg cacttcactg gccaccacgg caaagccacg cccctgttca ccggcgcggg
     9541 cggcctcaac ggcagcgttc aaggccagaa tgttggtctg aaaggcaatg ccgtcaatca
     9601 cactgatgat gtcggcaatc ttgcctgagg cttcgttgat gccgcgcatg gtgacca
//
LOCUS       DAOSLL010000103         9653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6813_length_9653_cov_4.360909, whole genome shotgun sequence.
ACCESSION   DAOSLL010000103 DAOSLL010000000
VERSION     DAOSLL010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9653
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6813_length_9653_cov_4.360909"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(384..1304)
                     /locus_tag="PLB25_06285"
     CDS             complement(384..1304)
                     /locus_tag="PLB25_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE41220.1"
                     /translation="MESYALLLAATLNAGTVLAIASLGLLINEKVGIVNLGAEGMMLC
                     AAIAGFATVVSTGSDWLGFAAGMAVGAALAAVFGLLVIWLNTNQYATGLALTLFGTGF
                     SAFVGTGFVQAKIPERSSFAVPYLSDIPLVGPALFRHHPLVYLTVVLALLLIFFMYRT
                     RSGLIMRSVGESPESAHALGYPVRRIRLLAVVAGGALCGLAGAYISVIYTPLWVEGMV
                     AGKGWIALALTTFATWRPARVLLGAYLFGGVTMLQFHLQGIGVDVPSQFLSMLPYIAT
                     IVVLALISRNPQWIRVNMPASIGKPFYPGA"
     gene            complement(1313..2371)
                     /locus_tag="PLB25_06290"
     CDS             complement(1313..2371)
                     /locus_tag="PLB25_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE41221.1"
                     /translation="MLKLEARAEPSGAWTYASPLLALGLTVIVGVLMFTALGKDPLRG
                     LEMFFWLPIKSGYPWGELLVKATPLLIIALGLAVCFRSNVWNIGAEGQYIIGAIAATG
                     VALLADGQSSRWLVLAVMLAGVVGGMAWAGITALLRDRFNANEILVSLMLVYVAVQVL
                     SYLVGGPWKDPMGFNFPQSKNFEAVARIPRLFDGSRVSIGVLLATAGVVGLWVFLFRT
                     RTGFSLQVGGLAPAAARYAGFSSRRALWAALLISGGAAGLAGALEVAGPIGQLTPYVP
                     AGYGFAAIIVAYVGRLHPIGMVLSAILMSMFYIGGEMAQSRLGLTKSLTGVFQGLLLF
                     SLLACDTLVNYRLIWRKG"
     gene            complement(2358..3941)
                     /locus_tag="PLB25_06295"
     CDS             complement(2358..3941)
                     /locus_tag="PLB25_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE41222.1"
                     /translation="MSPPRLQLSGITKRYPSVVANSDVSLTVLPGETHAVLGENGAGK
                     STLMKIIYGSVKPDEGRVLFNGQLVHIKNPQEARALGISMVFQHFSLFDTITVAENVW
                     LGLSKTLSLLDVTRSIESKATEYGLDIDPQRPVHTLSVGEMQRVEIIRALLTNPQLLI
                     LDEPTSVLTPQAVEKLFVVLKKLASEGCSILYISHKLHEIRELCNACTVLRGGRVTGV
                     CNPSQESNASLSKMMIGAEPPQLTHRAQEPGASVLRVANLILPREDQFGVDLEAISFE
                     VRSGEVVGIAGVSGNGQKELLCALSGEDVRAAAGTVRIGRIDVSKLHPGRRRRLGLYF
                     VPEERLGRGAVPTLSLAHNLLLTRQEAIAYRGFLGGWIRVKQLEAQAAGIIARFKVKA
                     GGPRSLARSLSGGNLQKFIVGREIDANPKLFIVSQTTWGVDVGAAAQIRGEILALRDA
                     GCAVLVVSEELDELFEVSDRLHVIARGQLSPSVATALATVEQIGEWMSGLWDHSVQAH
                     LGAKAGLVDGAQEADHAQA"
     gene            4127..5572
                     /gene="xdhA"
                     /locus_tag="PLB25_06300"
     CDS             4127..5572
                     /gene="xdhA"
                     /locus_tag="PLB25_06300"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004854 - xanthine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase small subunit"
                     /protein_id="HOE41223.1"
                     /translation="MHHPVLEFVHRNAVVSLREVPATRTLLQVLREDLGLTATKEGCN
                     EGDCGACTVVLGERQDGHIRYSAINACIRLAHSVHGMALWTAQDIAATDGTPHPVQQA
                     LLQNHASQCGFCTPGFAMSLFGLYQNQVCKGQPVSREQAQQALSGNLCRCTGYRPILD
                     AAQQMASLPPMLPAEAEILSKLELIAHTGQGLEADLSYQSPKTLAALLTLRAADPKAQ
                     IVAGCTDVGLWINKLHQDFASVLDLSQVAELRAISQDANTLSIGAAATLTDAFAALLI
                     ERPQLQSFMQRFAGLPVRNSGTLGGNVANGSPIGDSMPLLIALHARVVLASVRGSREL
                     ALEALYTGYRQNVIASDEVLTHIKVPRPTANEFLRVYKVTKRQEDDISAVCLALRIEL
                     HDGQLDCVSIGVGGVAAMPVRARQTEATLTGQVWTQALVDKAKTTLRGEFAPISDMRA
                     SSGYRTLVLGNLLQRFWLESQGQADVSLACV"
     gene            complement(5719..5841)
                     /locus_tag="PLB25_06305"
     CDS             complement(5719..5841)
                     /locus_tag="PLB25_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41224.1"
                     /translation="MSGYEQRRHPMSAAMTAFEALWYFIDRDGLRQQDQREISQ"
     gene            complement(5906..6352)
                     /locus_tag="PLB25_06310"
     CDS             complement(5906..6352)
                     /locus_tag="PLB25_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41225.1"
                     /translation="MFEEVATTSVYWWPLCKLTSVTRLGAHRPVICFSKSISKSSMSL
                     LSRLALRLQGMRQGALTRMTLKWNEVQLPNPNFRFWDATQSMHIADHLKTSQAQALYP
                     DCSSALDQWPHFDELSGRFFLLPIRVSRKNIFEFGGNQLWLVSRSR"
     gene            complement(6337..7866)
                     /locus_tag="PLB25_06315"
     CDS             complement(6337..7866)
                     /locus_tag="PLB25_06315"
                     /inference="COORDINATES: protein motif:HMM:NF025622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase domain-containing protein"
                     /protein_id="HOE41226.1"
                     /translation="MQNKLMIFDKRFEVDAICMRNFLFIFAFAILAKGPVIFRGYAID
                     DYFIASGVDSTSLAFVKSQGRYISAGIMWIFSSLGTNVNDMYFSLGLITLFLYSVFLV
                     SIFRFVGMSNSPAAGLVGAIMVAHPYLTEIFTFRMVLPYYCVALLFSIVALEALMRDP
                     AAWSSRLSASVATLAMLLTYQVFLNYLTVTVIFAFVCGLVLNGKGDSSEKSSIYNLRA
                     INLAVIILISSVVLIVIIRSANSLGLAGVDNRTSFIALGELPERLTELSSTLMKIYWI
                     AEPIFSGWLKVLVALTLVLSLGIIFRKLFVEKVKKNIFFVFVAIVLLIPASIGVIIVS
                     KDWWPVPRVLAHASIIVGLVFLLADECIQSSTSRFVKFPIFVSRIVILFGFVLLSNQI
                     LADQDKVNRWDRMMANRIILRLEMNPNFDDVKFIHISGGPWVYPKKLRTVQGDMNISA
                     LSMPYAKSGLLSEVSGYDFKWARTAREAIGDAYCAANQTWPHPDSVTVKDDLAIVCLR
                     K"
     gene            complement(8575..>9653)
                     /locus_tag="PLB25_06320"
     CDS             complement(8575..>9653)
                     /locus_tag="PLB25_06320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41227.1"
                     /translation="SDTAQTGFGFEDWQWNLETISKGARHVSALKTAHYYRRKVSSML
                     TGMVASGALIRPTAFFDHPERWNIRLRSHASPALTQVPDAQEQVMPQWAVQGFRNLAE
                     IEPSLNPTTEFLDKFFVYLHPFDLAPGELYQKAVSFIGAYEPDVICLLPWLCWGAADD
                     AAWQRLFSEHSAGKKVLVISSSCDDSTLNKDVPESYRLLEFGALSQSFSESNRIVVLA
                     RLILQSTASEIHVINSPVGWEVVKRHGKSFIATGKKIYASVHSVDSDSSEAVEFHTRE
                     ITQCLQESDAGSEAAKLQIEAWETLALKYAVAQEQEQEQEHEHEQNQPDEALTVDQPI
                     IGNKNSRIAARAKRLLSRVLGKQG"
BASE COUNT         2420 a         2814 c         2421 g         1998 t
ORIGIN      
        1 acctttttga tcagtccgat tgacttcaaa tgggagagtt gaccggcaag cgctgatggg
       61 gtcatcggca ccagcatcca tcagatcagc ccgtcgccaa catggatatt gagcgcgaca
      121 tgctgcaaga ctattgaaca ggcgatcccc agacggatcg aagcaattca gtctttttcg
      181 ggttgctctg agccagttga aacacaactg cctgcacgct ggacatgcgc gcgcgtaccg
      241 acacttcaaa caacccactc accaaactca actaactttc atggccgaga cggccacccc
      301 gcatcatgaa aaacttcttc gttcaaaatt attgaacata aattggtcga ttgctcgttg
      361 tttcacgtta aagctcatcg gccttatgcc cccggataaa acggcttgcc gattgatgcc
      421 ggcatgttga cgcgaatcca ttgcgggttg cgcgaaataa gggccagcac cacgatcgtg
      481 gcaatgtagg gcaacatgct caagaactgg cttggcacat cgacaccaat gccttgcaag
      541 tgaaactgca gcatcgtgac accaccgaac aaataggcac ccagcaaaac ccgtgccggt
      601 cgccaggtcg caaaagtggt caaggccagc gcaatccagc ccttgccagc caccatgccc
      661 tcaacccaca acggggtata aatgacagaa atataggccc ctgccagacc acatagtgcc
      721 ccacctgcca ccactgccag caggcggatg cgacgcaccg ggtaacctag tgcgtgcgcc
      781 gactctgggc tttcccccac gctgcgcatg atcaaaccgc tgcgcgttcg gtacatgaaa
      841 aaaatcagca gcagtgccag caccacggtc agataaacca gcgggtggtg ccgaaacaat
      901 gctgggccaa ccagcgggat gtctgacaag taaggcacgg caaaactgct gcgctcggga
      961 attttggcct gtacaaaacc ggtgccgaca aaggcagaaa aaccggtgcc aaacagggtt
     1021 aacgccagac cagtggcgta ttggttggtg ttgagccaga tcaccagcag accaaaaacc
     1081 gccgccagcg cggcaccaac ggccatgcct gcggcaaaac cgagccagtc gctgccggtg
     1141 ctgacgacgg tggcaaaacc ggcaatcgcg gcgcacagca tcatgccctc ggcaccgagg
     1201 ttgacgatgc cgaccttttc gttgatcagc aagcccagcg aggcaatcgc cagcaccgtg
     1261 cccgcattga gcgtggcggc cagcagcaag gcataagatt ccatggctta cctcaaccct
     1321 ttcgccatat caggcggtaa ttgaccaggg tgtcgcaagc cagcaggcta aacaacagca
     1381 gaccctgaaa cacacccgtc agtgatttgg tcaggcccag gcgagactgc gccatttcac
     1441 cgccgatgta aaacatgctc atcaatatgg cggacagcac cataccaatc gggtgcagcc
     1501 gcccaacgta ggcgacgatg atggcggcaa agccatagcc ggccggcaca tagggggtga
     1561 gctgaccaat cggcccggcg acttccagcg caccggccaa cccggccgca ccacccgaga
     1621 tcagcagcgc cgcccacagc gcccggcgcg acgaaaaacc ggcataccgc gctgccgccg
     1681 gtgccagccc gcccacctgc aaggaaaaac cggtgcgggt acgaaacaag aacacccaca
     1741 ggccgacgac accggccgtg gccagcagca ccccaatgct gacgcgcgag ccgtcaaaca
     1801 ggcgcgggat gcgcgccacc gcctcgaaat tcttgctttg cggaaaattg aagcccatcg
     1861 ggtctttcca cggtccgccg accaggtagc tcaagacctg caccgccacg tacaccagca
     1921 tcaggctgac cagaatttca ttggcattaa aacggtcacg cagcaaagcc gtgataccgg
     1981 cccacgccat gccgcccacc acaccggcca gcatgaccgc cagcaccagc catcggcttg
     2041 actggccatc agccagcagc gccacgccag tggcagcaat ggccccgatg atgtactgcc
     2101 cctcggcccc gatgttccag acgttggacc tgaagcacac agccagcccc agcgcaatga
     2161 tcagcagcgg tgtcgccttg accagcaact caccccaggg atagccgctt ttgatcggca
     2221 accagaaaaa catctccagt ccacgcagtg ggtcttttcc cagcgcggta aacatcagca
     2281 cgccaacgat cactgtcaga ccaagcgcca gcagcggcga cgcgtaggtc caggcaccag
     2341 acggctcggc gcgcgcttca agcttgagca tgatcggcct cctgcgcgcc atccaccagg
     2401 ccagcttttg cccccaagtg cgcctgcacc gagtggtccc acaagccgct catccattca
     2461 ccaatttgtt ccaccgtggc cagcgccgtc gccaccgagg gcgacaactg gccgcgggca
     2521 atcacatgca ggcggtcaga cacttcaaac agctcgtcaa gctcttcact gaccaccagc
     2581 acggcgcaac cggcatcgcg cagcgccagg atttcgccgc gaatctgcgc cgccgcgcca
     2641 acatcgaccc cccaggtcgt ttgcgagacg atgaacagct tggggttggc atcaatttcg
     2701 cgcccaacga tgaacttttg caaattgcca cccgacagcg aacgggccag tgatcgcggc
     2761 ccaccggcct tgaccttgaa acgggcaatg atgccggcgg cctgcgcctc cagttgtttg
     2821 acgcgaatcc agccgcccaa aaaaccccgg taagcaattg cctcctggcg cgtcagcaac
     2881 aggttgtgcg ccagtgacaa ggtcggcacg gcccctcggc ccagacgttc ttcaggcaca
     2941 aaatacaaac cgagcctgcg ccgtcggccc gggtgcagct tcgacacgtc aatgcggcca
     3001 atgcgcactg tgcccgctgc agcgcggaca tcttcgcctg acagcgcgca cagcaattct
     3061 ttttggccat tgcccgacac cccggcgatg ccgaccactt cacccgagcg cacttcaaac
     3121 gaaatggctt caagatcaac gccaaactgg tcttcccgtg gcaggatcag gttggccacc
     3181 cgtaacaccg acgcgccggg ctcctgggcg cggtgggtaa gctgtggtgg ctcggcacca
     3241 atcatcattt tggacagtga tgcgttgctc tcttgtgacg ggttgcagac cccggtaacc
     3301 cggccacccc gcaaaaccgt gcaggcgttg cacagttcac ggatttcatg cagcttgtgg
     3361 ctgatgtaca aaatgctgca gccctcgctg gcgagctttt tcagcaccac aaacagtttc
     3421 tcgaccgcct gcggggtcaa caccgatgtc ggctcgtcca gaatcagcag ttgcgggttg
     3481 gtcaacagtg cgcggatgat ttcgacgcgt tgcatttcac ccacgctcaa ggtatgcacc
     3541 gggcgctgcg ggtcgatgtc aaggccgtat tcagtggcct tggactcgat gctgcgggtg
     3601 acatcaagca ggctcagggt cttgctcaaa cccagccaga cgttttcagc cacggtaatg
     3661 gtgtcaaaca ggctgaaatg ctggaacacc atgctgatgc ccagggcacg cgcctcctgt
     3721 gggtttttga tgtggacaag ctgaccattg aacagcaccc tgccctcatc gggtttgacc
     3781 gatccataaa tgattttcat cagggtcgat ttgccggcac cgttttcacc cagcaccgca
     3841 tgggtttcac ccggcaaaac ggttagcgac acatcgctat tggccaccac gctggggtag
     3901 cgcttggtga tgccggagag ttgcagtctt ggcgggctca tgggcgggtg ggtgactcga
     3961 gttgtttcat caggccggta aacagtttgt accagactcc attctcaacg aaactgaaac
     4021 tgaatctgca ccaactggta tgcttcaaag caaataagat gccatcgtgg caggaaactt
     4081 gcggtgacgg cattgtttac cattgtcctt tttaattagg ccgaccatgc atcaccctgt
     4141 gcttgaattt gtccaccgca acgccgttgt ctcgttgcgc gaggtgcccg ccacccgcac
     4201 cctgctgcag gtactgcgcg aagacctggg gctgactgcc accaaagagg gctgcaacga
     4261 aggtgattgc ggtgcctgca ccgtggtgct gggcgaacga caggacgggc acatcagata
     4321 cagcgcgatc aacgcctgca tccgcttggc ccattcggtg catggcatgg ccctgtggac
     4381 tgcgcaggac attgccgcaa ctgacggcac gccacaccca gtgcaacaag cgttgctgca
     4441 aaaccacgcc agccaatgcg gcttttgcac gccgggtttt gccatgagcc tatttggcct
     4501 gtaccagaac caggtctgca agggccagcc ggtcagccgg gaacaggcac agcaagcgct
     4561 ctcaggcaac ctgtgccgct gcaccggcta ccggccgatt ctggacgcgg cacagcaaat
     4621 ggccagtctg ccaccgatgt tgccggcaga agccgaaata ctatcaaaac tggagcttat
     4681 tgcgcataca ggacaagggc tagaggctga tttgtcatat caatctccaa aaaccctggc
     4741 ggcattattg acgttgcggg cggccgaccc caaggcgcaa atcgtggccg gatgcaccga
     4801 tgtggggctg tggatcaaca agctgcacca ggattttgca agcgtgctgg acttgtcgca
     4861 ggtggccgaa ctgcgcgcca tcagccagga tgccaacacc ctgagcatcg gcgcggcggc
     4921 aacactcacc gatgcgttcg ccgcgctgct gatcgagcgc ccgcagttgc agagcttcat
     4981 gcagcgtttt gccggcctgc cagtgcgcaa ctcgggcacg ctcggcggca atgttgccaa
     5041 tggctcgccg attggcgact caatgcccct gctgattgca ctgcatgcca gggtggtgct
     5101 ggccagcgtg cgcggcagcc gcgagctggc gctggaggcg ctctacaccg gctaccggca
     5161 aaacgtcatc gccagcgacg aagtgctgac gcacatcaag gtgccgcgcc cgacggccaa
     5221 cgagttcttg cgtgtctata aagtcaccaa gcgccaggag gacgatattt ctgcggtctg
     5281 tctggcgctg cgcatcgagc tgcacgacgg ccagcttgac tgcgtgtcga tcggtgttgg
     5341 cggtgttgcc gcaatgccgg tgcgggcacg ccagaccgag gccacattga ctggccaggt
     5401 ctggactcag gccttggtgg acaaggccaa gaccaccttg cgtggcgaat ttgcgccgat
     5461 atcagacatg cgcgccagca gcggttaccg cactctggtg ctgggcaatc tgctgcaacg
     5521 cttctggctg gaaagccagg ggcaagccga tgtcagcctg gcctgcgttt gaatggccgc
     5581 aagatcaagc aacccgtcgg tcaaccaaaa acagactacc gggcacacca agccggatgg
     5641 ccaaggcgtt cgacggataa cgcaagttgg gggatttttg cctactgaga atttctgaca
     5701 tatcggcctg attgccgatc actggctgat ctctcgctga tcttgttgcc gcaaaccgtc
     5761 acggtcaatg aaataccaca acgcctcgaa ggccgtcatt gcagcggaca tcgggtggcg
     5821 acgctgctcg taaccagaca tcaagcaagc gccacagtcg gcaagcccgc gagcgggtgc
     5881 aactcatgag cgccacctgc atccgttagc ggctgcggga aacaagccac aactgattgc
     5941 caccaaactc aaaaatattc ttgcggctta cccttatcgg cagcaaaaaa aaacggccac
     6001 ttagctcgtc aaaatgaggc cattgatcca acgcactact gcaatcagga tacagcgctt
     6061 gcgcctgact ggttttcaaa tggtcggcga tgtgcattga ttgcgtcgca tcccaaaacc
     6121 ggaaattcgg gtttggaagc tgcacctcat tccatttcaa agtcatccgt gtcaaggcac
     6181 cttggcgcat accttgcaag cgcaaggcaa ggcgagacaa caacgacatg gacgatttgg
     6241 aaatggattt ggaaaaacaa attacgggcc gatgtgcacc aagcctcgtt accgaagtca
     6301 atttgcaaag tggccaccaa tacactgaag tagtggctac ttcctcaaac aaacaatcgc
     6361 caagtcatct ttgacagtta ctgaatctgg gtgcggccaa gtctgattgg ccgcacagta
     6421 ggcatcccca atagcctccc tagcagttct tgcccattta aaatcatatc ccgaaacctc
     6481 tgatagcaag ccacttttgg cgtaaggcat cgataaggct gatatattca tatcgccttg
     6541 cacagttctc agctttttcg gatataccca tggacctccg ctgatatgaa taaatttgac
     6601 atcatcaaaa ttaggattca tttcaaggcg caaaattatt ctatttgcca tcattctatc
     6661 ccaacgattg actttgtctt gatcagcaag aatttgattg cttagcaaca caaacccgaa
     6721 aagaataaca atcctggaaa caaagatcgg aaatttaaca aaacggctgg ttgaactttg
     6781 aatacattca tccgccagta aaaaaacaag tccgacaata atacttgcgt gcgcaagaac
     6841 gcggggaacg ggccaccagt ctttcgatac aataatgaca cctattgaag caggaatgag
     6901 caacacgatt gccacaaata caaaaaatat attttttttg actttttcta caaacaactt
     6961 cctgaaaata atgccgagtg ataagacaag cgtcagggca accaacacct ttaaccatcc
     7021 tgaaaaaata ggctcagcaa tccaataaat tttcatcaag gttgacgata actctgttaa
     7081 acgctcaggt aactcgccta aagcgataaa gctggttctg ttgtcgacgc cagccagacc
     7141 caaactatta gctgatcgaa taataacaat caaaacaaca gaagaaatga gaataataac
     7201 tgccaagttt attgctcgca agttataaat actacttttt tccgatgaat cgccttttcc
     7261 atttaaaacc agaccgcaaa caaaagcgaa gataactgta acagtcaaat aattcagaaa
     7321 aacttgataa gtaagaagca tcgcaagcgt tgccactgat gctgataatc gcgagctcca
     7381 cgctgcagga tccctcatca aagcttcaag tgccactata gaaaacagaa gcgcaacaca
     7441 gtaatatggc aataccattc gaaaggtaaa tatctcagtc aagtatggat gcgcgaccat
     7501 gatggcccca accagacctg ctgctggtga attcgacatc ccgacaaatc ggaaaattga
     7561 tacaagaaag actgaataca aaaataatgt tatcaagccc aatgaaaagt acatatcatt
     7621 gacatttgta ccaagtgagc taaatatcca cattattccc gctgaaatgt aacgcccttg
     7681 agatttaaca aatgccaaac ttgttgaatc aacgccggat gcgataaaat aatcgtcaat
     7741 cgcatatcct cgaaaaataa caggcccttt tgccagtatc gcaaacgcaa aaataaacaa
     7801 gaaatttcgc atgcaaattg cgtcaacttc gaatcgttta tcaaaaatca tcaatttatt
     7861 ttgcatcgat ttatcccgtt aatctgagga ttgcgcagta agcaatacaa atgagccgac
     7921 cacaatttgg ataagatgtc tgaaacctat ggtggcaatg caaccgtttg gacccgtcat
     7981 tttgatcacg ccgtcattgc caatgattgc caatgatcct ggattcctcc gtccgacacg
     8041 cccgaatgcc ccgctggcag cgcggctggg tggggcgaaa gctgcggctc caaacttgcc
     8101 tgtgcaggtt tgaacggctt gaaccgagat tcctccgttg accgcctgtt tcatctgtaa
     8161 gctctcatga atattgattt ttttcagctt cgttggggtt cgcccaatgg gtgacagcgc
     8221 tacgcaaccg cctgagccac tggcaacacg ctggggtcgt tatgccttcc ctcgtccgcc
     8281 aggaccacgt gcactcaggc gcccgctgcg tcgtcgcgcc tggtcgcgat gccagcggcc
     8341 cactcggtcg cttccaaatg aaaccaggtt gaagtgcttt atcttggcct tgtgtattga
     8401 caggcttaat caaaaaccga aattgatcac ataaatcctt attattttca tttcagaggg
     8461 tgtcaatttc caattcttga aaactattaa ggtggcttgc gttatcaata ggtcgctatt
     8521 gcacctgtgc acgatgtttg atcgggcaaa tggtatttcg tttggatctg gcaactaccc
     8581 ctgcttaccc aacactcgcg aaagaagacg ctttgcacgg gcggctatac gagaattttt
     8641 gttaccaatt atcggttgat caactgtcaa cgcctcatca ggttggttct gttcatgttc
     8701 atgctcttgc tcttgctctt gctcttgcgc aactgcatac ttcaacgcca aggtttccca
     8761 agcttcgatt tgcaatttgg ctgcttcgct tcctgcatca ctttcttgca agcattgcgt
     8821 aatttctctt gtgtgaaact ctactgcttc acttgaatct gaatccacgc tatgcacact
     8881 cgcgtaaatt ttttttcctg ttgcaatgaa cgacttacca tgacgcttta ccacttccca
     8941 tccaaccggg gaattgatga catgtatttc agaggcagtt gactgcagga taagtcgtgc
     9001 cagcaccacg attcggttgc tctcggaaaa actctgggaa agtgctccaa actcaagcaa
     9061 gcgataagat tctggaacgt ctttgttgag cgtcgagtca tcgcatgaac tcgaaatcac
     9121 aagcaccttc ttgcctgctg aatgttcgga aaaaagccgc tgccacgccg cgtcgtctgc
     9181 tgcgccccag caaagccatg gcagcaggca aataacgtct ggctcatagg cgccaatgaa
     9241 agacacagct ttttgataga gttcacccgg tgccagatca aatggatgga ggtacacaaa
     9301 gaacttgtcc agaaactcag tggtcgggtt caggcttggc tcaatttctg ccaaattgcg
     9361 aaacccttgc accgcccatt ggggcatcac ctgctcttgg gcatccggca cctgcgtcaa
     9421 ggcgggcgaa gcgtgggacc gcaatctgat gttccaccgc tctggatggt caaaaaatgc
     9481 tgtcggccga atcaaagccc ctgatgccac catcccggta agcatcgaag aaacttttcg
     9541 tctgtaataa tgagccgttt tcaatgcact cacgtgcctg gcacctttgg agattgtttc
     9601 cagattccat tgccagtcct cgaatccaaa accggtctgc gcagtgtccg atc
//
LOCUS       DAOSLL010000104         9647 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6822_length_9647_cov_4.184425, whole genome shotgun sequence.
ACCESSION   DAOSLL010000104 DAOSLL010000000
VERSION     DAOSLL010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9647
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6822_length_9647_cov_4.184425"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(224..1753)
                     /locus_tag="PLB25_06325"
     CDS             complement(224..1753)
                     /locus_tag="PLB25_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HOE41228.1"
                     /translation="MSLSLVQSRALLGLEAAAVTVEVHLANGLPSFTLVGLADVEVKE
                     ARERVRSAIQNSGLEFPNNKRIIVNLAPADLPKDSGRFDLPIALGILAASGQIDTQAL
                     AGFEFAGELSLSGELRPVRGALAMSLALHAEQVVTQLVLPPDSAEEAALVPTAQVFRA
                     RHLLDVVQHFLPTPVAAAAPSDGWTRIQTTPRVQTAQYPDLADVKGQLGAKRALEIAA
                     AGGHSLLMMGPPGSGKSMLAQRFAGLLPPMTTDEALQSAAVASLGGRFSLDKWAQRPT
                     CHPHHTASAVALVGGGSPPRPGEISLAHHGVLFLDELPEFPRAALEALREPLESGSIT
                     ISRAARRSDFPARFQLIGAMNPCPCGYLGSTQKACRCTPDQVARYQSKLSGPLVDRID
                     LHVEVPAVGPGELLQAPAGESSAAIQARCTAARERSIARQGKTNQALQGQEIDQFVLL
                     DEAAAKFLNVAAARLGWSARSTHRALKVARTIADLVGASTTQMTHVAEAMQYRRALRT
                     S"
     gene            2008..2346
                     /gene="glnK"
                     /locus_tag="PLB25_06330"
     CDS             2008..2346
                     /gene="glnK"
                     /locus_tag="PLB25_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HOE41229.1"
                     /translation="MKLVTAIIKPFKLDEVREALSSIGVQGITVTEVKGFGRQKGHTE
                     LYRGAEYVVDFLPKVKIEAAVADELVDQVIESIEGAARTGKIGDGKIFVYPVEQVIRI
                     RTGETGKEAL"
     gene            2395..3948
                     /gene="amt"
                     /locus_tag="PLB25_06335"
     CDS             2395..3948
                     /gene="amt"
                     /locus_tag="PLB25_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOE41230.1"
                     /translation="MKKLLASLALGIGLLLGSSATMAQAPAASEAAPATLAASAAAPA
                     TSEVKAADAPAMASATVAASAPAAAAPVPVPNKGDTSWMMVSTLLVIMMSIPGLALFY
                     GGLVRSKNMLSILMQVMVTFSMVVVLWFIYGYSLAFTEGNAFFGGFDRLFMAGVWDNA
                     AGTFANAATFSKGVYIPEIVFAVFQASFAAITCCLIVGAFAERMKFSAVLMFALLWFT
                     FSYAPIAHMVWFWMGPDAYATKDVVDAMTGKAGYLWQSGALDFAGGTVVHINAAVAGL
                     VGAYMVGKRIGYGKESMAPHSLTLTMVGAALLWVGWFGFNAGSALEANGFAALAFVNT
                     FLATAAAVLSWCIGEALMKGKASMLGAASGCVAGLVAITPACGNVGIGGALVIGLLAG
                     FAGLWGVNGLKKLLGADDSLDVFGVHGVCGILGAILTGVFNSPALGGPGFVADWVTVS
                     MVSAADYSIASQVWIQTKAVLITVVWSGLVSVVAYKIVDMTIGLRVSEEGEREGLDIT
                     SHGEAAYGR"
     gene            complement(4164..4379)
                     /locus_tag="PLB25_06340"
     CDS             complement(4164..4379)
                     /locus_tag="PLB25_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41231.1"
                     /translation="MRLLLVPSCGTCTQHHPARVVRRGGGCSCVGVLSGGTFGVDRLE
                     AVWACGKPDMFNSNPRGAQFTWCCASP"
     gene            complement(4376..4918)
                     /locus_tag="PLB25_06345"
     CDS             complement(4376..4918)
                     /locus_tag="PLB25_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41232.1"
                     /translation="MNTTDTALPDDQNPPTPPKKKSRYVEQRERISAVRRANFAKLCD
                     KVGTKEMMEKMEWETPSQRSQLLTGNNGRAFPESMARRIEWAFSLEVMSLDKDTGDVH
                     PMPVKFVPAGTNPFSQNFDGNTLATAMDIVGQVIAETSTKLTAEKQSILSRVVFELVG
                     VNGWKIDRDLIGLLVNLGRL"
     gene            5782..6600
                     /gene="imuA"
                     /locus_tag="PLB25_06350"
     CDS             5782..6600
                     /gene="imuA"
                     /locus_tag="PLB25_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HOE41233.1"
                     /translation="MALCSVSSLLEDFGAVVWRADALARSASADAAALPSGHAELDAR
                     LPGGGWPVGALCEILQTADAQCEWRLLLPALRRLGQTVVLVGPPHAPFGPGLAAQGLN
                     VQALLWVKADGLADRLWAAEQVLRCAGVDALLLWLPKARADHLRRLHLAAQSHAKLLF
                     VMRPATAQDESSPALLRLGLSACAPAPTDIKSPASAAVVDHGAGFAADALLVQVLKRR
                     GPPLAQTLNVRARHAPLSVLLALRDDGYRNTRSACGDLAPDRGGHALDRLAATA"
     gene            6509..8005
                     /locus_tag="PLB25_06355"
     CDS             6509..8005
                     /locus_tag="PLB25_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HOE41234.1"
                     /translation="MTATATPDLRVAILRPTGGVMHWIALQPQPEAALPEPLAVLGWW
                     ALQFTPKVALLADAVLLEVSASERLWRGRAALLRHIYTSNKPVALAGYARGATSLVAL
                     GRLLAAQPGSTPADALPLSALLAAQPHLATLGRIGCRTWGELRALPRDGLARRFGADL
                     LAALDQAYGTRPDVYPWLTLPEVFEARLELLTHIDSAPAMLFGARRLLAQLQVWLQLR
                     QQGVLAIELGWTMDARRNTGNAGALLVRTAEASAQTAHLQRLLAERLAQVTLAAPVQS
                     LRLRSVETRALVGQSASLLPEEQLPGDSLAQTIERLSARLGPDKVLRLQARADHRPEH
                     RQAWVAATDTITSIAVSANKTRATGQKGSKHRAQTSGLTGDWASPLCPTWLLAEPLKL
                     AVFQQRPHYQGPLKLLSGPQRLEAGWWPLHADAAKPDGSDELALGPGKVAHSRSAAPS
                     PANPGLALRDYFVARSASSALLWIYRERLPGADAAQSSSAWYLHGIFA"
     gene            complement(8094..>9647)
                     /gene="ggt"
                     /locus_tag="PLB25_06360"
     CDS             complement(8094..>9647)
                     /gene="ggt"
                     /locus_tag="PLB25_06360"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HOE41235.1"
                     /translation="AANLALIGAAVLAVVYPAAGNLGGGGFMTLQLADGRKTFLDFRE
                     KAPLAATANMYLDQQGNVIKGLSTYGHLAVAVPGSVSGLEYAREKYGTKKRTELLKPA
                     IRLAQYGFTLNQGDLAILQTATDAFRKDPASAALFLKHGQPWQAGDKLVQKDLAETLQ
                     AVSQRGADGFYKGPVAAAIVKSSQSGGGIITQADLDQYRTRELAPVECDYRGYHVVSA
                     PPPSSGGVVICEMLNILEGYPLKELGFRSAQSVHYQIEAMRHAYVDRNSYLGDPDFVS
                     NPVARLTDKAYAAQIRAVIDPAKAGVSKDLKPGVEPHEGSNTTHYSIADRWGNAVAVT
                     YTLNDWFGAKVTAPGTGVLLNNEMDDFTAKVGVPNLYGLVQGAANAIAPGKRPLSSMS
                     PTIVSQAGQPLLVLGTPGGSRIITAVLHTLLNVIDFDMTLQEAVDAPRFHQQWLPDLT
                     SVENFAFSPDTRKLLEAAGHKLGPAQPANHMTAVMIGAPSLGGTPVGKNRFYGANDPR
                     RNTGLALGY"
BASE COUNT         1726 a         2916 c         2992 g         2013 t
ORIGIN      
        1 agttggacgc gcccgagtgg gtcgctggcg gcgcgtctgg gtaggcgcga cgacgacgtg
       61 ggctcctgcc cgctaggagg agcaacgccc ccaggcgcgt tgccagtggt tcaatcgggt
      121 gcgtagcgct gtcacccgat gggtgaactc catcgaagtt aaaaaagtaa atgctcataa
      181 ggacttactg ctgaaaacaa gcgtccaact gaggaatcaa ggtttaactt gtgcgcagcg
      241 cgcggcggta ctgcatggcc tcggccacat gggtcatttg cgtggtgctg gcaccgacca
      301 ggtcggcaat ggtgcgcgcc accttcagcg cccggtgcgt gctgcgggct gaccagccca
      361 gccgtgccgc ggccacattc aagaacttgg cggcagcctc atcgagcagc acaaactgat
      421 cgatctcctg gccttgcagc gcctggttgg tcttgccctg gcgggcaatc gaacgttcgc
      481 gcgcggcggt gcaacgcgcc tgaatggccg cgctggactc acccgctggc gcctgcaaca
      541 gttcgccggg cccgaccgca ggcacttcaa catgcaagtc aatgcggtcc accaacggtc
      601 cgctgagctt gctctggtag cgtgcgactt ggtctggcgt gcagcggcag gccttttggg
      661 ttgaacccag atagccgcac ggacagggat tcatggcacc aatcaactga aaacgcgccg
      721 gaaaatcgct gcgccgtgcg gcccgcgaga tggtgatgct gcccgactcc agcggctctc
      781 gcagcgcttc cagcgcggcc cgggggaatt ctggcaattc gtccagaaac agcaccccat
      841 ggtgcgccag cgagatttca cccggcctcg gcggcgaccc accaccgacc aacgccaccg
      901 cgctggcggt gtggtgcgga tggcaggttg gccgctgcgc ccatttgtcc agcgaaaagc
      961 ggccacccag gctggccaca gcggcacttt gcaacgcctc atcggtggtc atcggtggca
     1021 gcaagccggc aaaacgctgt gccagcatcg acttgcccga gcctggcggc cccatcatca
     1081 gcaggctgtg gccacccgct gcggctattt ccagtgcgcg tttggcaccc aactgccctt
     1141 tcacatcggc cagatcaggg tattgcgcgg tctgtacccg tggcgtggtc tggatgcgcg
     1201 tccagccgtc ggatggcgca gccgcagcca ccggtgttgg caaaaaatgc tgcaccacgt
     1261 cgagcaaatg gcgcgcccga aacacctgtg cagtaggcac cagcgcggct tcttcggcgc
     1321 tatcgggcgg caacaccagt tgcgtcacca cctgctcggc atgcagggcc aggctcatgg
     1381 ccaaggcacc gcgcaccggc cgcagttcgc ccgacagaga caactcgccg gcaaactcga
     1441 agcctgccag cgcctgagtg tcgatctgtc cacttgccgc cagaatgccc agcgcaatgg
     1501 gcaaatcaaa gcgaccggag tctttgggca gatcggccgg ggccaggttg acgatgatgc
     1561 gtttgttgtt ggggaattcc agccctgagt tttgaatggc ggagcgcacc cgctcacgcg
     1621 cttctttcac ctccacatct gccagaccca ccagggtaaa gctgggcagt ccgttggcca
     1681 ggtggacctc gaccgtgact gcggcagctt caagccccag cagggctcga ctttgaacca
     1741 aagacagact catacgctca tcctcgctaa aaaggtgcgc agccatcggt gccccttcaa
     1801 tttgatgcac cagcaaggtg catactgttt atttgtacag atatcttact gcaaaccgga
     1861 tggtgcgtgg ggtctttcca ggaatgcagg cttccagcat gtgtggcacg aaccctgctt
     1921 taccgcctta cctatcgcat cgttggagat atccatgcca ttgaaatcac ccactaccca
     1981 tttgtctgcc cacgccttgg agaaatcatg aaacttgtca ctgccatcat caaacctttc
     2041 aagctcgatg aggtgcgcga agcgctgtcc tccatcggcg tgcagggtat tacggttacc
     2101 gaagtcaagg gctttggtcg ccaaaagggt cacaccgagc tgtaccgtgg tgctgaatac
     2161 gtggtcgatt ttttgcccaa ggtgaagatc gaagccgcag tggctgacga actggtggac
     2221 caggtgatcg agtccatcga gggtgccgcg cgcaccggca agatcggaga cggcaagatt
     2281 tttgtctacc cggtcgagca agtcatccgc atccgcaccg gcgagaccgg caaggaagcc
     2341 ttgtaaacaa ccgccggttt gacccttcaa tccagcaccc caattggaaa tcacatgaaa
     2401 aaactactcg catccctcgc gttgggcatt gggctgctgc ttggcagcag cgccaccatg
     2461 gcgcaagcgc ctgcggcctc tgaagccgcg ccagccacat tggcagcttc ggctgcggca
     2521 cctgccacat cggaagtcaa agccgccgat gcaccggcaa tggccagcgc cacggtcgcc
     2581 gcatcagccc cggctgccgc tgccccggtg ccggttccca acaagggcga cacctcctgg
     2641 atgatggtgt ccaccctgct ggtgatcatg atgtcgatac cgggtctggc ccttttttac
     2701 ggtggcctgg tacgcagcaa gaacatgctt tccatcctga tgcaggtgat ggtcactttt
     2761 tccatggtcg tggtgctgtg gttcatctac ggctacagcc tggcgtttac cgagggcaac
     2821 gcgttctttg gcggctttga ccggctgttc atggctggtg tctgggacaa cgcggctggc
     2881 acttttgcca acgcggcgac gttcagcaag ggggtctata tcccggaaat cgtgtttgcc
     2941 gtgttccagg ccagctttgc tgccatcacc tgctgcctga ttgtcggggc ctttgccgag
     3001 cgcatgaagt tctctgccgt gctgatgttc gcgctgctgt ggttcacctt cagctacgca
     3061 ccgattgccc acatggtctg gttctggatg ggtccggatg cttacgccac caaagatgtg
     3121 gttgatgcca tgaccggcaa ggccggttac ctctggcagt ccggtgcgct tgactttgcc
     3181 ggcggcacgg tggtgcacat caacgctgcc gtggccggcc tggtgggtgc ttacatggtt
     3241 ggcaagcgca ttggttacgg caaggaatcg atggcgcctc acagcctgac cttgacgatg
     3301 gtcggtgctg cactgctgtg ggtcggctgg tttggcttta acgcaggctc ggcccttgaa
     3361 gccaacggtt ttgccgcact ggcatttgtc aacaccttcc tggcaaccgc tgctgccgtg
     3421 ctgtcctggt gtatcggtga agccttgatg aagggcaaag cctcgatgct cggtgcggct
     3481 tctggctgtg tcgccggtct ggtggcgatc acgccggctt gcggcaatgt cggtatcggt
     3541 ggcgcgctgg tgattggctt gctggctggt tttgccggtt tgtggggtgt caacggcctg
     3601 aagaaactgc ttggtgctga cgactcgctc gatgtctttg gtgtccacgg tgtctgcggc
     3661 attttgggtg cgatcctgac cggtgtcttc aacagcccgg ccctcggtgg ccctggcttt
     3721 gttgccgact gggtgaccgt gtcgatggtg agcgccgctg attactcgat tgccagccag
     3781 gtctggattc agaccaaggc cgtgttgata accgttgtct ggtctggcct ggtgtcggtg
     3841 gtggcctaca agattgttga catgacgatt ggcctgcgtg tcagcgaaga aggcgaacgt
     3901 gaaggtctgg acatcacgtc ccacggagag gccgcttacg gccgttgatc gggttggttg
     3961 ctgcaggccg tagcgtctgc agcttattgt cgaatgagtt gcttccttga gagttgaacc
     4021 ccccgtcgcc taacggcttc ggggggtctt tttttggcct ttcagactgg ccaatgcctg
     4081 gatggccgcc acgcgctggc gcggccaatc aggccaccca ttcgcgccag cggctggctg
     4141 ggtgtcaaac ggtgctgtcc ggttcatgga ctggcacaac accaggtgaa ttgcgcaccc
     4201 ctggggttgc tgttgaacat gtcaggcttg ccgcaggccc acacagcctc caggcggtca
     4261 acaccaaaag tgccacccga cagcaccccg acgcaactgc agccgccacc ccgacgcacc
     4321 acacgggccg gatggtgctg ggtgcaagtg ccacaactgg gcaccaggag aagcctcata
     4381 gccgacccaa gttaaccagc agacctatca ggtcacggtc gatcttccat ccgttgacac
     4441 caaccagttc aaacacgact cgggacaaga ttgactgctt ttcagcagtc aacttggtac
     4501 tcgtttcggc aatcacctga ccaacgatgt ccattgccgt tgcgagtgtg ttgccgtcaa
     4561 aattttgaga gaacggattt gtgccagcag gcacaaactt gaccggcatc ggatgcacgt
     4621 cgcccgtgtc tttgtccaaa gacatcacct ccaggctaaa cgcccactca atcctgcgcg
     4681 ccatcgactc ggggaatgca cgcccgttgt tgcccgtcag gagttggctc cgttgactcg
     4741 gtgtttccca ctccattttt tccatcattt ccttggtgcc gactttatcg cagagcttcg
     4801 cgaagttcgc ccggcggacc gcactgatgc gttcacgttg ttcgacgtat ctggacttct
     4861 ttttaggtgg tgtgggcggg ttttggtcgt ccggtaaagc ggtgtcggtc gtattcatta
     4921 caagttcttt tctgataatt tagcaattgg aacgcaattg aaaagtttat tacgcaaaat
     4981 gtaacaataa tcgttagcct ttgcaacaaa aatgattagg taagaacccc tactttttag
     5041 gtgtttgagc aacacttgcg caaacagttc aaaataaact tggggtgagt tgttgcgcca
     5101 atgctaaaaa acatgcgaaa acacgcgata atcggggcat gagaaccaat gcacccctaa
     5161 ccccactgct tgccatcctg cgggcgatga cccctgacca gcgggaggag ttcgcgctgg
     5221 tggcgggcac gtcagtgtca tacctgtacc agcttgccgg gtgcaaccgc aagtcctgcc
     5281 gggccaaact tgccaagggc atatcccatg cgaccgggca catgcaccgg cgctacgaca
     5341 cgccaacgat cagcatggag gcgttggcag gcagtgctat ctgccggatg tcgggcaata
     5401 aggctcctgt ccctgttcag cggcatcaag gctgtaagca ccgcatgagc cgatctcggc
     5461 tgggagtgag tgggcttcag cgagatagag ccgttcccct gtgagttgct gacacagcgc
     5521 catccaagtg tgcccaactt gggggacgtg accaagatca ccgaaacagg tattttgatg
     5581 tttggtcaga gtgaccttct tgtacatcgg ctgaccctgt tgtcgctgct atcagcaccc
     5641 tgcagtgcca tgccgcgcgt atgtgcccgg ctagattccg accggatcaa aaaacctgag
     5701 gataccggcg accctctcaa ataactgttt atttatccag ttaaatggtg tctaatggct
     5761 ttttgagggt gctgttgtgt catggctttg tgttctgttt catccctgct ggaagatttt
     5821 ggcgctgtcg tctggcgtgc cgatgcactg gcgcgctcgg catctgctga cgctgcggca
     5881 ctgcccagcg gccacgccga actcgatgcc cggttgcctg gtggtggctg gccggtaggc
     5941 gccctgtgcg agattttgca aacagcagac gcgcagtgtg agtggcgttt gctgttgcca
     6001 gccttgcgca ggctcgggca aacggtggtg ctggtgggcc cgcctcatgc tccctttggg
     6061 ccgggtttgg cggctcaggg tctgaatgtg caggccttgt tgtgggtcaa ggcggacggg
     6121 ttggcagacc ggctgtgggc cgccgagcag gtcttgcgct gcgccggggt tgatgccctg
     6181 ctgctctggt tgcccaaggc gcgcgctgac catctgcgcc gcctgcacct ggctgcccag
     6241 tcgcatgcca agctgctgtt tgtgatgcgc ccagccaccg cccaggacga gtcatcacct
     6301 gcgctgctgc gccttggtct gagcgcctgc gcaccagcgc caactgacat caagagcccg
     6361 gccagtgcgg ctgtggttga ccatggtgcc ggttttgctg ccgatgcctt gctggtgcaa
     6421 gttctcaagc gccgtggccc gccgctggcg cagacgctca acgtgcgtgc gcgccacgca
     6481 ccgttgagcg tgctgctggc cttgcgcgat gacggctacc gcaacacccg atctgcgtgt
     6541 ggcgatcttg cgcccgaccg ggggggtcat gcactggatc gccttgcagc cacagcctga
     6601 agcggcgttg cccgagcccc tggccgtgct gggctggtgg gcgttgcaat tcaccccaaa
     6661 agttgcgctg ctggccgatg cggtgttgct ggaggtgtcc gccagcgaac gcctgtggcg
     6721 tggccgggct gccctgctgc gccacattta cacgtcaaac aagcctgtag cccttgccgg
     6781 atatgcgcga ggagctacat ctttggtagc attagggcgc ttgctggcag cccagcctgg
     6841 cagcaccccg gccgatgctt tgccgctgtc cgcactgctg gctgcgcagc cccatctggc
     6901 gacgctcggg cgcatcggct gccgcacctg gggcgagttg cgggcactgc cacgcgacgg
     6961 cttggcacgt cgttttgggg ctgatctgct ggctgcactg gatcaggctt acggtacgcg
     7021 gcctgatgtt tatccctggc tgacgctgcc cgaggtgttt gaagccagac tggaattact
     7081 cacccacatc gacagcgcgc ctgcgatgct gtttggcgca cgccgcttgc tggcgcaatt
     7141 gcaggtgtgg ctgcaactgc gccagcaggg cgtgctggcg atagaactgg gctggaccat
     7201 ggacgcgcgg cgcaacaccg gcaatgcggg cgcactgctg gttcgcaccg ctgaggcaag
     7261 cgcccagacc gctcatctgc agcgcctgct ggctgagcgg ctggcgcagg tgacgctggc
     7321 agcaccggtg cagtcgctgc gcctgcgctc ggttgaaacc cgggcgctgg tcggccagag
     7381 tgccagcctg ctgcccgaag aacaattgcc cggtgacagc ctggcgcaga ccatcgagcg
     7441 cctgagcgca cgtctggggc cagacaaagt gctgcgcctg caagcccgtg ccgaccaccg
     7501 gccagaacac cgacaagcct gggtggcggc tacagacact ataacttcaa tagctgtcag
     7561 cgcaaacaag acaagggcta caggtcaaaa aggttcaaaa catcgggcgc agacaagtgg
     7621 cttgaccggt gactgggcca gcccactatg cccaacctgg ctgctggctg agccgttgaa
     7681 gctggcagtg tttcagcagc gcccgcacta ccaggggccg ctgaaattgc tgagcggccc
     7741 gcagcggctg gaggccggtt ggtggccgtt gcacgctgat gcagccaagc cagacgggtc
     7801 tgacgagctg gctttgggac ccggcaaggt tgcgcacagc cgttcagccg cgccgtcccc
     7861 ggccaatccg ggtctggccc tgcgcgacta ttttgtggcc cgcagtgcca gttcagccct
     7921 gctgtggatt taccgcgagc gcctgccggg tgcagacgct gcgcagtcat cttcggcgtg
     7981 gtatttgcac ggcatctttg cctgaaaacc ggcttgcagg ctgaagcacc cggcaattgc
     8041 cccagcttgg ccagccctgc ctgatggctt gcaccgccag ttcacgcatg aactcagtag
     8101 cccagcgcca gcccggtgtt gcggcgcgga tcattggcac cgtaaaagcg gtttttgccg
     8161 accggtgtgc ctccgagcga cggtgcgcca atcatcacgg cggtcatgtg gttggcgggt
     8221 tgcgctgggc cgagcttgtg accggccgct tccagcagct tgcgggtgtc gggcgagaag
     8281 gcgaagtttt ccaccgatgt caggtccggc agccactgct ggtgaaagcg cggcgcgtcc
     8341 accgcctctt gcagcgtcat gtcgaagtcg ataacgttga gcagcgtgtg cagcacggcg
     8401 gtgatgatgc ggctgccccc aggtgtgccc aacaccagca gcggctggcc cgcctggctg
     8461 acgatggtcg ggctcatcga cgacaggggc cgcttgccgg gggcaatcgc gttggccgcg
     8521 ccttgcacca gaccatacag gttgggcaca cccactttgg cggtgaagtc gtccatttcg
     8581 ttgttcagca gcacaccggt tcccggggct gtgaccttgg caccaaacca gtcattgagc
     8641 gtgtaggtca cggcgaccgc gttgccccag cggtcggcaa tcgagtaatg cgtggtgttg
     8701 ctgccctcgt gtggttcgac gccaggcttg aggtctttcg agacacccgc cttggcgggg
     8761 tcgatgacgg cacgaatctg ggccgcatag gccttgtcgg tcaagcgggc caccgggttg
     8821 ctgacgaaat ccgggtcgcc caggtaactg ttgcggtcca cgtaggcatg acgcatggcc
     8881 tcgatctggt agtgcaccga ctgggccgag cgaaagccca gctctttgag cgggtagcct
     8941 tccagaatat tgagcatttc gcagatgacg accccgcccg agctcggtgg cggtgccgag
     9001 acgacgtgat aaccccggta atcgcattcg accggggcca actcgcgggt gcggtactgg
     9061 tccaggtcag cctgggtgat gatgccgccg ccactttgac ttgacttgac aatggcggcc
     9121 gcaaccgggc ctttgtaaaa cccatccgca ccgcgctggc tgacggcctg cagtgtttcg
     9181 gccaggtctt tttgcaccag cttgtcaccg gcctgccagg gctggccgtg cttcaggaag
     9241 agggcggcac ttgccgggtc tttgcgaaac gcatcggtgg cggtctgcaa gatggccaag
     9301 tcgccctgat tcagggtgaa gccatattgc gctagtctga tggctggttt gagcagctcg
     9361 gtgcgttttt tggtgccata tttttcacgc gcgtattcca gccccgagac gctgcccgga
     9421 accgccaccg ccagatggcc gtaggtgctc aaacccttga tgacattgcc ttgctggtcc
     9481 agatacatgt tggcggtggc tgccagcggt gccttttcgc gaaagtccag aaaggtcttg
     9541 cgaccatcgg ccaactgcag cgtcatgaag ccgccaccgc cgaggttgcc tgcggccgga
     9601 taaaccaccg ccagcaccgc cgcgccgatc agcgcgagat tcgccgc
//
LOCUS       DAOSLL010000105         9643 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6827_length_9643_cov_3.764602, whole genome shotgun sequence.
ACCESSION   DAOSLL010000105 DAOSLL010000000
VERSION     DAOSLL010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9643)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9643)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9643
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6827_length_9643_cov_3.764602"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLB25_06365"
     CDS             complement(<1..252)
                     /locus_tag="PLB25_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41236.1"
                     /translation="MKHRKQYQNNSYQRLSLLGCTLFLSTIFGGCSSLPQTVSVDFKD
                     HSLAEASPRTAHVAKGPATGSLFQKASYRPAFEDARARLV"
     gene            complement(268..1050)
                     /gene="flgG"
                     /locus_tag="PLB25_06370"
     CDS             complement(268..1050)
                     /gene="flgG"
                     /locus_tag="PLB25_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HOE41237.1"
                     /translation="MINSLWIAKTGMEAQQMQLDVISNNLANVSTNGFKRSSAVFEDL
                     MYQNLRQVGANSTEQSTLPTGLQIGLGVRTVATSRSFAQGNLLQTSNKLDVAIQGEGF
                     FQVTMPDGTISYTRDGAFQVNSAGALVTATGLAVANGVTVPATATSVTIDGNGTVTAA
                     IPGTTTPASIGTIALARFINPAGLTPLGGNLYAESAASGQPTAGTAGADGIGTLMQGF
                     VETSNVNVVTELVTMIQTQRAYEMNSKAIQTSDQMLQKLAQL"
     gene            complement(1070..1810)
                     /locus_tag="PLB25_06375"
     CDS             complement(1070..1810)
                     /locus_tag="PLB25_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgF"
                     /protein_id="HOE41238.1"
                     /translation="MDRLIYTAMTGANAAAYRQAVLSNNLANVSTNGFRAELSTYRAV
                     PVRGDGSTTRVMALEATPGFLETPGTPQRTGRALDAMATGNAWFAVQGLDGTEAYSRN
                     GSFEVASDGTLKTSNGLTVLSDGGAPINVPAGGEVTLGSDGTVSSKVGKQPSTGIGRL
                     KLATPTAEDPLKRSNDGLFRTTSGDPMPNDANAKLEPGVLEGSNVNAIETMVGMIQSA
                     RQFEAQTRLMQTAEADDRAAAQLLSLQG"
     gene            complement(1849..3210)
                     /gene="flgE"
                     /locus_tag="PLB25_06380"
     CDS             complement(1849..3210)
                     /gene="flgE"
                     /locus_tag="PLB25_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HOE41239.1"
                     /translation="MSFQTGLSGLNASSRSLDVIGNNIANANTTGMKSSRAEFGDLVA
                     SSLGAGGSSGPGIGVAVDTIAQQFTQGNISTTGNSLDVAINGSGFFQLAQTDGSAAYT
                     RDGSFKLDKDGYIITNSGANLMGLPTDADGVPTSGTIEALKLPTSAPIAAKQTDSVML
                     RMNLDARATPAVGVAASVGPPAVAAIEPTARSTYATSVNVYDSQGVASPITLYFEKDV
                     AANTWNVFTSLDTTLFPSPTGIFQITFDTSGKITAPASPTSTAVPPLNPLPAFGFELP
                     AGSITSLNPNEYVSGAPTDGFLDQPIMLDLGQSGNGDTTAVTQYGTSFAVADLTQDGY
                     TAGELTSVNIGEDGMITTRYSNGQTQSGGMITLADFRNVQGLSPLGGNAWAETFASGQ
                     PVQGSAATGRFGSLQSGALEDSNVDLTAELVNMMTAQRAYQANAQTIKTQDQIMSTLV
                     NLR"
     gene            complement(3240..3911)
                     /locus_tag="PLB25_06385"
     CDS             complement(3240..3911)
                     /locus_tag="PLB25_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook capping FlgD N-terminal
                     domain-containing protein"
                     /protein_id="HOE41240.1"
                     /translation="MLTGTTASTVSTNSTIAGTTSTDSTSTSTSAIDEAQDMFLTLLV
                     AQLNNQDPMNPMDNAEMTSQMAQINTVTGIQQVNDTLKSMADQFAAMQVIQGSNMVGH
                     EVLIDGNTLTVKDGKAGGAIDLSAQAESVKVEILSPGGLVLDTLNLGALDAGRHNFEW
                     DASKYTESGSPTFKVTATLGGKAIDSTSMARATVSSVSSEAGVMKVQLANGTSIAYTS
                     VKAIL"
     gene            complement(3927..4331)
                     /gene="flgC"
                     /locus_tag="PLB25_06390"
     CDS             complement(3927..4331)
                     /gene="flgC"
                     /locus_tag="PLB25_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HOE41241.1"
                     /translation="MSMFSIFSVSGSAISAQSQRLNVVASNLANADAVAGPDGQGYKA
                     RQVVFQTTPLGSDAAAGVKVSSIQESNEPFKRVHNPNHPSADAEGYVNYSNVNPVEEM
                     VNMISASRSYQNNIEVMNTAKSLLLKTLQMGQ"
     gene            complement(4358..4813)
                     /gene="flgB"
                     /locus_tag="PLB25_06395"
     CDS             complement(4358..4813)
                     /gene="flgB"
                     /locus_tag="PLB25_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009429 - bacterial-type flagellum basal
                     body, proximal rod [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HOE41242.1"
                     /translation="MFANLTRGLDFHSKALVLRAERQHIIASNIANADTPGYVARDIN
                     FRDALRQSTGTSSSDFSAASKQLSLSKSNASGTVQPRHIALTGSADSQLTTTSNLSYV
                     AQTQPSVDSNSVDLDRERASFVDNSVRYESTLRFINGQSKTLLSAIQGQ"
     gene            4996..5730
                     /gene="flgA"
                     /locus_tag="PLB25_06400"
     CDS             4996..5730
                     /gene="flgA"
                     /locus_tag="PLB25_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HOE41243.1"
                     /translation="MIIQSLTLMRTLALVWLCLGELLTTSVLAQPVVVADNAAIYGDA
                     QSWLNQEVSNTSGLPLRMEVTVGELDRRLKLAACDQIEPYLPPGTRLWGKTRLGLRCL
                     QGSVKWNVFLPITVKAFGPAWVIKGQVASGTLLTESDVMMVEVDWAESNSPVAANLTD
                     WLGLTATRTLTTGQTLRHDMVRAAQVFQAGAQVRVVARGAGFEIATSAQAISGGVVGQ
                     SARVRMDNGRVVSGMVLDNRTVRLDI"
     gene            5827..6153
                     /gene="flgM"
                     /locus_tag="PLB25_06405"
     CDS             5827..6153
                     /gene="flgM"
                     /locus_tag="PLB25_06405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HOE41244.1"
                     /translation="MKISQPLDNPVAVSNNSVAQATRNGTMASTLVKTGAPKSAASAG
                     VAVSVSDLTRSLESASLGETSDVDTAKVSFVRNAIAQGTYAVNPEAIADKMLSNVMET
                     LQGNGS"
     gene            6197..6583
                     /locus_tag="PLB25_06410"
     CDS             6197..6583
                     /locus_tag="PLB25_06410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41245.1"
                     /translation="MKNISHIELLNQIEAKVEAFANALQSNRLEELVSVAVSFQMACV
                     ELSSCMQKVPTDDVGTRRKIGQVAALMAAQRVALMRRSMLVERGLATLMPAVHAETYV
                     PSAGRFSSNLYASAGRRSGEFRTSVA"
     gene            complement(6599..7315)
                     /locus_tag="PLB25_06415"
     CDS             complement(6599..7315)
                     /locus_tag="PLB25_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HOE41246.1"
                     /translation="MYTAKGQLDRNALIRQYQPLVRKLAHHMMVRLPANVQVDDLIQV
                     GLIGLSEALSRFEANQGVQFETFATQRIRGAMLDELRENDWVSRGTRKSQKEIEEAMR
                     RLEHRLGRSPLESEIAADLKMSLADYQTLLGKVQGTQLVYLEDLSRGQEDDDNFLDRH
                     MGDADADPLSMLRDQRLRTALVAAIKNLPEREQFIMSMYYEQDMNLKEIAAVLDVTES
                     RVCQLHSQSIARLRAKMRTH"
     gene            complement(7315..8058)
                     /locus_tag="PLB25_06420"
     CDS             complement(7315..8058)
                     /locus_tag="PLB25_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41247.1"
                     /translation="MLEPCVNQAAGLQILAPHAAPRLVGVTSHGQQQGELPLLWGLCN
                     TWVDMGFSVLVLDGHSQESAQNPGLSQLLDNPSGRLNETEGAISWSVLPASLGFDRLA
                     SQGLSSSALGDLFQNFAVVLIYADAGTMSKLLKRSGLEPLLIVAPLMSSSLTAYQALK
                     QLLLDAQLRPTVANIVLSPVANATMPNTFNAQHLQHCAMTFLGYAIRPITITASARSD
                     SSQDDVNRLALQLLENAVLLERHPIERVH"
     gene            complement(8051..9496)
                     /gene="flhF"
                     /locus_tag="PLB25_06425"
     CDS             complement(8051..9496)
                     /gene="flhF"
                     /locus_tag="PLB25_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhF"
                     /protein_id="HOE41248.1"
                     /translation="MNIQRFTAPTAREALAKARLAFGEGTLILSNRPTGSGVEVVATG
                     EETLATLDRNELARQGKRPAVAQPVAQATANPDSQVVDDATQLAMSTLSFQDYVRERM
                     LRRRHEAMSGQVQLKELTLPQAVQPIRIDAPRAERPAARSTPTQYAPAAAQVRATPRP
                     AAVPAPAVSQGIVDELQAMKALIEERFNTLTWLGQTRQNPVQSNLMLKLLRAGYSPVL
                     ARTIIERLPEQTSADDAARWVMDVLIRNLHTDANAQPIHDEGGTFALVGATGVGKTTT
                     AAKLAGLCARNHGANSVGLITLDTYRVGAHEQLRAYGRMLGVVAHLAHDRAALQDLLG
                     LLANKKMVLIDTTGIAPNDPRKRDMLDVLDIPEVNRLLVLNAGGHGDTLDDVIASFKT
                     NGVQQSILSKIDEAAKLAPALDAIIRHQLQLRGVTMGQKVPEDWERADAGKLVGMSMR
                     TPVKSAFDPKPTDLGFFFAQSTPLHPGQLDA"
BASE COUNT         1886 a         2561 c         2992 g         2204 t
ORIGIN      
        1 gaccagtcgc gcgcgcgcgt cctcaaaggc cgggcggtac gacgctttct ggaacaggct
       61 gccagtcgcc ggccctttgg ccacgtgggc agtccgcggg ctggcctcag ccaagctgtg
      121 gtccttgaag tccaccgata ccgtctgggg caaggacgag cagccaccaa aaattgtgga
      181 caaaaacagg gtgcagccca atagggacaa gcgctgatag ctattatttt gatactgttt
      241 gcggtgcttc atgatgaacc tcaaagttca gagttgggcg agcttttgca gcatctggtc
      301 ggatgtctgg atggcttttg agttcatttc ataagcacgc tgggtctgga tcatggtgac
      361 cagttccgtt accacgttga cgttggaggt ttcaacaaag ccctgcatca gcgtacctat
      421 gccgtcggca ccggcggtgc cggcagtcgg ctggcccgat gctgcgcttt ctgcgtacag
      481 gttgccgccc agaggcgtca gtccggccgg gttgataaag cgggccaacg cgatggtgcc
      541 aatgctcgcc ggggttgtgg tgcccggaat ggctgccgtc accgtgccat tgccatcaat
      601 ggtgaccgag gttgcagtgg cagggaccgt gacaccgttg gcgacggcca ggccggttgc
      661 agtcaccagc gcaccggccg agttgacctg aaacgcgccg tcgcgggtgt agctgatggt
      721 gccatccggc atggtcacct gaaagaaacc ctcgccctga atcgcaacat caagcttgtt
      781 gctggtttgt agcagattgc cttgcgcgaa agagcggctg gtagccaccg tgcggacacc
      841 gagtccaatt tgcaggccgg ttggcagtgt tgactgttcg gtgctgttgg ctcccacctg
      901 gcgcaggttc tggtacatca ggtcttcaaa cacggcagag gaacgtttga agccgttggt
      961 agagacattg gcgagattgt tggaaatcac atccaactgc atttgctggg cttccatgcc
     1021 ggtcttggca atccacaggg agttgatcat ggtggtgtcc taaaaaatat cagccttgca
     1081 agctgagcag ttgagcggcg gcgcggtcgt cggcttctgc cgtttgcatc aggcgggttt
     1141 gcgcttcgaa ctggcgggca gactggatca tgccgaccat ggtctcgatg gcgttgacat
     1201 ttgagccctc aagcacgccg ggctccagct tggcgttggc atcgttgggc atggggtcgc
     1261 ccgaggtggt gcgaaacagg ccatcgttgc tgcgcttgag cgggtcttct gcggtggggg
     1321 tggccagctt gagtcggcca atgccagtgg aaggctgttt gccaaccttg gaactcacgg
     1381 tgccgtccga gcctagcgtg acttcacctc cggcaggcac attgatgggg gcgccgccat
     1441 cgctaagtac ggtcagacca ttgctggttt tcagcgtgcc atcggatgca acttcaaacg
     1501 agccattgcg ggagtaggcc tcggtgccgt cgagtccctg aacggcaaac catgcgttgc
     1561 cagtggccat cgcatccagg gcgcggccgg tgcgctgggg tgtgccggga gtctccagaa
     1621 agcctggggt tgcttccagc gccatgacac gggtggtgga gccgtcgccg cgcaccggaa
     1681 cagcccgata agttgacagt tcggcccgaa agccattggt ggacacattg gccaggttgt
     1741 tggacagcac ggcctgccga tacgcagcag cattggctcc ggtcatggcg gtgtagatca
     1801 gtcggtccat gctcgctccc ggtcaaaatg gtttcacgct gacgaacatc aacgcaggtt
     1861 gaccagagtg gacatgatct ggtcttgtgt cttgatggtt tgcgcgttgg cctggtaagc
     1921 gcgttgtgcg gtcatcatat tgacaagctc cgccgtcagg tctacgttgg agtcttccag
     1981 ggcaccagat tgcagcgaac cgaaacgccc agttgccgcc gagccctgca ccggctggcc
     2041 ggaggcgaat gtctcggccc aggcattgcc gcccagcggt gacaagccct gcacattgcg
     2101 aaaatcagcc agggtgatca tgccgcccga ctgtgtttga ccattggagt agcgcgttgt
     2161 gatcatgccg tcctcgccga tgttcacact ggtcagttcg cctgcggtgt agccgtcctg
     2221 tgtgaggtct gccaccgcga agctggtgcc gtactgggtg acagccgtgg tgtcgccatt
     2281 gccgctttgg cccaggtcga gcatgatggg ctgatcaagg aagccgtcgg tgggtgcacc
     2341 ggagacatac tcgtttgggt tgagtgaggt gatggatccg gcgggtagct caaagccgaa
     2401 agcgggcaag gggttgagtg gcggaacggc agtgctggtt ggtgaggccg gagccgttat
     2461 tttgccgctg gtgtcaaaag tgatctgaaa gatacccgta ggtgaaggga ataaagtcgt
     2521 gtcaaggctg gtaaatacgt tccaggtatt ggctgccacg tctttttcaa agtagagggt
     2581 gatgggtgat gccacgccct gcgagtcgta aacattgact gaagtggcgt aagttgaacg
     2641 agctgtcggc tcaatggcgg ccactgcagg agggcccacc gatgccgcaa cgccgactgc
     2701 aggggttgcc ctggcatcca gattcatgcg cagcatgact gagtccgttt gttttgctgc
     2761 aatcggtgca ctggtgggta gcttcaatgc ttcaatcgtc ccgctggtgg gcacaccgtc
     2821 tgcatcggtg ggcaagccca tcagattggc gccactgttg gttatgatgt aaccatcttt
     2881 gtcaagtttg aaagagccat cgcgggtgta ggctgcagac ccgtctgttt gggcaagctg
     2941 aaagaagcca ctcccgttga tggctacatc caggctgttg ccagttgtcg agatattgcc
     3001 ctgggtgaat tgctgcgcaa tggtgtccac tgcgacaccg atgccaggtc cgctgctacc
     3061 gccggccccc agtgacgatg ccaccaggtc gccaaactcg gcgcgcgatg atttcatgcc
     3121 agtggtgttg gcgttggcga tgttgttgcc gatgacatcc aggctgcggc tggaggcatt
     3181 gagtcccgag agtccggttt ggaaagacat ggtaatttcc tggttagctt ggagatggtt
     3241 taaagaatgg ccttgacgct ggtgtaagcg atggatgtcc cattggccag ttgcaccttc
     3301 atgacgccgg cttcgctgct gaccgaactc accgtggcac gtgccatgct ggtgctgtca
     3361 atcgctttgc ctcccaaagt ggcggtgacc ttgaaggtgg ggctaccact ttcggtgtat
     3421 ttggaggcat cccactcgaa gttgtggcga cccgcatcca gggcaccgag gttcagggtg
     3481 tcgaggacca gaccaccggg ggacagaatt tctaccttga cgctttcagc ctgtgcgctc
     3541 aggtcaattg cgccacccgc tttgccgtct ttgacggtca gggtgttgcc atcaatcagc
     3601 acctcgtgcc caaccatgtt ggagccctgt atcacctgca tggcggcaaa ctgatcggcc
     3661 atgctcttga gtgtgtcgtt gacttgctgg atgccggtaa ccgtgttgat ctgtgccatc
     3721 tggctggtca tctcggcgtt gtccatcggg ttcatcgggt cctggttgtt cagttgtgcc
     3781 accagcagtg tcagaaacat atcttgcgct tcgtcaatgg cagaagttga ggtgctcgtg
     3841 ctgtcagtgc tggttgtgcc ggcaatggtc gagttggtgg aaacggttga ggcagtggta
     3901 ccggtcagca tggtggtgtc cttgtgttat tgacccattt gcagcgtctt gagcagcagg
     3961 gatttggcgg tgttcatgac ttcgatgttg ttctggtagg aacgcgaggc cgagatcatg
     4021 ttgaccattt cttctactgg attgacattg gagtagttga cataaccctc ggcatctgca
     4081 gaggggtgat tcgggttgtg cacccgtttg aacggctcgt tgctctcttg aatcgagctg
     4141 actttgacac cggctgcggc atcagagccc agtggagtgg tctggaaaac cacctggcgc
     4201 gccttatagc cctgtccgtc aggacccgcc acggcatcgg cgttggccag gttgctggcc
     4261 accacgttga gtcgctgcga ctgggcgctg atggcgctac ctgatacgct gaatattgaa
     4321 aacatggaca tggtggtgtt tcccctgcgg ctatttgtta ttgaccctga atcgcgctga
     4381 gaagggtctt ggattgccca ttgatgaagc gcagggtgga ctcgtaacgt accgagttgt
     4441 ccacaaagct ggcccgctcg cggtcaaggt ccacgctgtt gctgtccaca ctgggctggg
     4501 tttgggcgac atagctcagg tttgatgtgg tggtcaactg gctgtcagcg cttcccgtca
     4561 gagcgatatg tctcggctgg acagtgccgc tggcgtttga cttgctcagg ctcagttgct
     4621 tgcttgcggc tgaaaagtcg ctgctgctgg tacctgtgga ttggcgcagg gcatcccgga
     4681 aattgatgtc ccgagccaca tagcctggcg tgtcggcgtt ggcgatgttg ctggcaatga
     4741 tgtgctgacg ttcggcacgc aacaccaagg cctttgaatg gaaatccaga cctctggtta
     4801 agttggcaaa catggagcac ctcgactttc tcgcccggtg ggtgttgacc cgggaagccc
     4861 tgaattgatt gatgagcgat tatgaaaatc cggtgcagtt tctaaactga aaatagcagc
     4921 aaaaaggccg ttcacttgtt gcgttctgtc gacccggcgt tggctacatt tgtttggtct
     4981 gctactggaa ccgtcatgat cattcaatct ttaactttga tgcgcacgct tgcactggtg
     5041 tggctgtgcc tgggtgaact gttgaccaca tcggttcttg cgcagccggt ggttgtagcc
     5101 gacaacgccg ctatttacgg tgacgcgcaa agttggctga accaggaggt atcgaatacc
     5161 agtggcctgc cgctgcgaat ggaggtcact gtaggggaac tcgatcgccg actgaaactg
     5221 gcggcttgtg accagattga gccttatctg ccgcccggca cccgcttgtg gggcaaaacc
     5281 cgtctggggc tgcgctgttt gcaaggatcg gtcaagtgga atgtgttttt gccgatcacg
     5341 gtcaaagcat tcgggccagc ttgggtcatc aagggccagg tggcatccgg taccttgttg
     5401 accgagtccg atgtcatgat ggtggaggtg gattgggctg aaagcaacag tccggtggcc
     5461 gcgaatctga ctgactggct gggtttgacg gcaacccgga ctctgaccac cgggcaaacg
     5521 ctgcgtcatg acatggtcag ggctgcccag gtgtttcagg ccggtgcgca ggtgcgggtg
     5581 gtggcccgag gtgctggctt tgagatcgcg accagcgctc aggcgatctc tggaggcgtt
     5641 gttggacaga gtgcacgcgt gcgaatggac aacgggcgag tggttagcgg aatggttctg
     5701 gacaatcgga cggtcaggtt ggatatatga aggcttctgt cgttctgaaa atagcgttaa
     5761 agtctgcgct gtttctgccg aaagtgaact tgacaggcta catccatcgt agctgtggag
     5821 aacaccatga agatcagcca gccgttagac aatcctgtag cagtcagcaa caactcagta
     5881 gcccaggcca ccaggaatgg gaccatggca agcacgcttg tgaaaacggg cgcacccaaa
     5941 tctgccgcat ccgccggagt ggccgtgtcc gtgtcggacc tgactcgttc tctcgaatct
     6001 gccagcctgg gcgagacatc cgatgtcgat acagccaagg taagttttgt acgcaatgcg
     6061 atcgcccaag gcacctatgc cgtcaatccg gaagcgattg cagacaagat gctgtccaat
     6121 gtgatggaga cgctccaagg gaacggttcg taagcgcttt agcctcgact tttcctctgc
     6181 tacctgagga aaagtcatga aaaatatctc tcatattgaa ctgctgaatc agatcgaagc
     6241 caaagtggag gcatttgcga atgcgctgca gtcaaatcga ctggaagaac tggtaagcgt
     6301 tgcggtctcc ttccaaatgg cttgtgtcga actttccagt tgcatgcaaa aggtgccgac
     6361 tgacgacgtg ggcaccaggc gcaagatcgg tcaggtggct gccttgatgg cggcccaaag
     6421 agtggcactg atgcgcaggt ccatgttggt tgagcgtgga ttggcgaccc tgatgccggc
     6481 cgttcacgca gaaacttatg tgccatcggc gggccgattt tccagcaact tgtatgccag
     6541 cgctggccgt cgcagtggtg aattcaggac atcagtagcc tgactggtca cgccgaagtc
     6601 agtgtgtgcg catttttgcc cgtaaccggg cgatagactg gctgtgcaac tgacataccc
     6661 gcgattcggt tacatccagc actgcggcga tttccttcag gttcatgtct tgctcgtagt
     6721 acatgctcat gatgaactgc tctcgctcgg gcaggttttt gatggcagca accaatgccg
     6781 tgcgcagacg ttgatctcgc agcatactga gtggatccgc atcagcatca cccatgtggc
     6841 ggtccagaaa attgtcatcg tcttcctgac cgcggctcaa gtcttccagg taaaccaatt
     6901 gtgtgccctg gaccttgccc agcaaagtct gatagtcagc caggctcatt ttcagatcag
     6961 cggcgatttc actttccagg ggactgcgtc cgaggcggtg ctccaggcgg cgcatggcct
     7021 cttcaatttc tttttgactc ttgcgcgtac cacgtgaaac ccaatcgttt tccctcagct
     7081 cgtcgagcat ggcaccccgg atgcgctggg tggcaaaggt ctcgaactgc acaccttgat
     7141 tggcttcgaa acgcgacagc gcctcggaca ggccgatcaa gcccacctgt atcaaatcat
     7201 ctacctgaac atttgcgggc agcctgacca tcatgtgatg ggccagtttg cgcaccagcg
     7261 gttggtactg ccgaatcagg gcgttgcggt caagctggcc cttggctgtg tacatcaatg
     7321 caccctttca atggggtgtc gctcaaggag caccgcgttt tccagcagtt gcagcgccaa
     7381 ccggttgaca tcatcttgcg aactgtcaga ccgagcggat gccgtgatgg tgatgggtct
     7441 gatggcataa cccagaaagg tcatggcgca gtgctgcaag tgttgggcat tgaaagtgtt
     7501 gggcatcgtc gcgttggcaa ccggggacag cacgatgtta gcaacagttg gccgcaactg
     7561 ggcatccagc agcaattgtt ttagtgcctg gtaggccgtc aatgaagacg acatgagtgg
     7621 tgccacgatc agcaggggtt caagtccact gcgcttgagc agcttgctca tggtgccagc
     7681 gtcggcatag atcagcacca cagcgaaatt ctgaaacaga tcccccaaag cagaggaaga
     7741 aagcccctga gacgcgaggc gatcaaaacc aaggctggcg ggcagcactg accatgagat
     7801 agcgccctca gtctcattga gcctgcccga cggattgtcg agtaactgcg acaagccggg
     7861 gttttgcgcg gactcctggc tgtgtccatc aagcaccagc acggaaaacc ccatgtctac
     7921 ccaggtattg catagacccc acagcaatgg cagctcaccc tgctgttgtc cgtgactggt
     7981 gacaccgacg agtcgcggtg ccgcgtgtgg tgccaggatc tgcagaccgg cggcctgatt
     8041 gacgcagggt tcaagcatcc aattgccccg gatgcaatgg tgtggactga gcaaaaaaga
     8101 agcccagatc tgtgggtttt ggatcaaatg cagatttcac tggtgtccgc atcgacatgc
     8161 caaccagttt gccagcatct gcgcgctccc agtcttcggg cactttttgg cccatggtga
     8221 caccgcgcaa ctgcagttgg tggcgaatga tcgcatcaag cgccggcgcg agctttgctg
     8281 cctcgtcgat tttggagaga atactctgct gtacgccatt ggttttgaag gatgcaatga
     8341 catcgtcaag cgtatcgcca tgcccgccag cattgagcac cagcaagcgg ttgacttccg
     8401 gaatgtccag cacgtcaagc atgtcacgtt tgcgtgggtc gttgggggca atgccggtgg
     8461 tgtcgatcag taccattttt ttgttcgcca gcaaccccag caagtcctgc agcgcagcac
     8521 ggtcgtgtgc caagtgtgcc accacgccca acatgcgtcc ataagcacgc agttgttcgt
     8581 gtgcacctac ccgataagtg tcaagggtga tcagcccgac actgttggca ccgtggttgc
     8641 gagcgcaaag cccggccagc ttggctgcgg tggtcgtttt gccaacgccg gtggcaccca
     8701 ctagcgcaaa cgtgccaccc tcatcatgta tcggttgtgc gttggcatcg gtatgcaaat
     8761 tgcgaataag gacatccatg acccagcggg cagcatcgtc agcggatgtc tgctcgggca
     8821 gtcgctcaat gatggttctt gccagaaccg gtgagtagcc agcacggagc agcttgagca
     8881 tcaggttcga ctgaaccgga ttttgccgcg tctgccccag ccaagtcagg gtgttgaagc
     8941 gttcttcaat cagggctttc atggcttgca gctcatcgac gataccctgc gacactgccg
     9001 gagcgggaac cgcagccggg cgcggcgttg cgcgtacctg agctgcagcc ggtgcatact
     9061 gagtcggagt ggaccgtgct gcgggacgtt ctgcgcgtgg ggcatctatc cggatgggct
     9121 gtaccgcctg cggtagggtc agctctttga gttggacctg cccggacatg gcttcatggc
     9181 gacgacgcag catgcgttct cgcacatagt cctgaaacga cagcgtactc atggccagtt
     9241 gcgtggcatc gtctaccacc tgactgtcgg ggttggcggt ggcttgcgcc acaggctggg
     9301 cgactgctgg tcgcttgcct tgacgcgcca gttcattgcg gtcgagtgtt gccagggttt
     9361 cttcgccggt ggcgaccact tccacgccag atccggtggg gcgattggac agaatcaagg
     9421 tgccttcgcc aaaagccagt cgtgccttgg ccaatgcctc gcgggcagtt ggggcggtaa
     9481 agcgctgaat gttcatgatg ctgcaccttt aaggatagga ccgatgcgaa tggtgtggga
     9541 ttcagggatt tcactgtgcg cgagaacttg tagtctgggt gccacccgcc gcaccaggcg
     9601 ggcaatggca ctgcggattt ggtcgggtac cagcacacac gcg
//
LOCUS       DAOSLL010000106         9565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6885_length_9565_cov_7.463302, whole genome shotgun sequence.
ACCESSION   DAOSLL010000106 DAOSLL010000000
VERSION     DAOSLL010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9565
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6885_length_9565_cov_7.463302"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     repeat_region   74..821
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=74..101
                     /rpt_unit_seq="cttctcaaccacctgcacggtgggtcac"
     gene            complement(912..1883)
                     /gene="cas1f"
                     /locus_tag="PLB25_06430"
     CDS             complement(912..1883)
                     /gene="cas1f"
                     /locus_tag="PLB25_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - endodeoxyribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated endonuclease Cas1f"
                     /protein_id="HOE41249.1"
                     /translation="MTEKKAPRPQVKPRAVMLSKRANVFYLEHVRVIQKDGRIVYITE
                     DGNLIERYFNVPERNTAFILLGKGSSITDAAARRLAESNVIVGFCGTGGSPLFSTVDY
                     AFLTPLSEYRPTEYMQEWMRAWLDEERRLGLARNLLLKRCDIIQTSWAKMPELLQRGL
                     AVPLPLLDKLRHEVAAGASTEALLLSEARFAKSLYAVLANTYRIEFSREEGKGSSQST
                     ADIINGFLDHGNYIAYGYAAVALNGLGISFSLPLLHGKTRRGALVFDVADLIKDAFVM
                     PMAFHCGKQGESSRDFRQMLIEKLQEEEVLDLLFGFVKSLCEKSVAN"
     gene            complement(1880..2428)
                     /gene="cas6f"
                     /locus_tag="PLB25_06435"
     CDS             complement(1880..2428)
                     /gene="cas6f"
                     /locus_tag="PLB25_06435"
                     /inference="COORDINATES: protein motif:HMM:NF021157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated endoribonuclease
                     Cas6/Csy4"
                     /protein_id="HOE41250.1"
                     /translation="MLQYTQIDLLSRAEETSPKPILLARLMRILHGVFQSKPETFAIA
                     LPSHGFSSVRVFARDRTELDFLAGAIQSHWFVRDYTRLGYPAAVPADYAGAWVSYRRY
                     RIPSAKSDRNAQARQPTLGQRRSAQAQKDGLVYLISDSQSNQQKFSLFVQSMAAAGPG
                     EFRPNSYGLSSSKRAFALPDVP"
     gene            complement(2428..3510)
                     /gene="csy3"
                     /locus_tag="PLB25_06440"
     CDS             complement(2428..3510)
                     /gene="csy3"
                     /locus_tag="PLB25_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy3"
                     /protein_id="HOE41251.1"
                     /translation="MTTAIQKSAVPFKKLPGVLSLQRGMILSDAMFYSLMPGGERAPL
                     YVMRHGIRGTQNVNESKSDDAAASVAGAKGREVSNIQQTDSAKLAPEASALEVRFDVR
                     FADLSGALFACAPSKSDTMDDINKFKETLSEFIERAKQGDALDLLSLRYARNIANGRW
                     LWRNRMISESINVTASIGSSQLQFDALAVPLNHFDMPTERESDLAKVIKEGFCGQRNA
                     LIKVCAELKFGVQGPLEVFPSQNYLEAKEKGFARSLYQLGNAPQDQDKHGILFLGQAA
                     LRDQKIGNALRTIDTWYPAFAQRGVAIAVEPNGASLDAQMFFRSKKTSGFDILRHVGS
                     LDPASDDGQFLLACIIRGGVYSGGDV"
     gene            complement(3514..4299)
                     /locus_tag="PLB25_06445"
     CDS             complement(3514..4299)
                     /locus_tag="PLB25_06445"
                     /inference="COORDINATES: protein motif:HMM:NF021154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy2"
                     /protein_id="HOE41252.1"
                     /translation="MTYLVIPSMRVHGANLLTSNLVAGGPPLMACALFAHQVAREAGA
                     RDLGFFLIHHDRQELGQFIYGRLHGQQRRGAMFIDGSDYSSTNKQALSLQPTLSAHLR
                     VSVVIKFDDLQDVDAVHKMLERGKFSGGTVLSHGQIETEETLQAAFKRVPSGFVLRDK
                     SIELEQPGESRAAVLIKKLAANPQLLDAHPPWLAATTLGYATLTEFKHKGGVREGVEH
                     AFAEPLTGLVQYVSLRKAASELKSLMWACAWPHPDVFLLSQRS"
     gene            complement(4296..5507)
                     /locus_tag="PLB25_06450"
     CDS             complement(4296..5507)
                     /locus_tag="PLB25_06450"
                     /inference="COORDINATES: protein motif:HMM:NF021151.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy1"
                     /protein_id="HOE41253.1"
                     /translation="MNEKIEEYESSFRGFVHFSFKTVNSNAPAGGIRIDAADALNLPY
                     VSARTLLKEGISCMTDYTKNSGRIATHFKHAKQARMDASGIIPKKKADKDGAKSAEKE
                     DAPDLAPDELLAVGDALARCTQGRLEDGCDAFLDPRLRQLLLPYTDETGAVSYRSVTP
                     LTAGGLCQIMRAHVAASNEAHKLGKEDWQKEKEKALKKNPKAVIARLATRSVQLTQLG
                     IGGSNPQNVGGLVREMQQPMYMGAPQAKGSLRQALAMHHKGVDLNISLKLVRKYADFL
                     KANTEDDVRQTAMESREEEKSILAEFVDDVMDQVDDIRRVLDQHLEMLPADTTGKHMY
                     FTQKLHHNVNAAWVDESLRGRDWASLAAVWLANAIGKFEVQTKNGPKRLLSLDVFGIK
                     SIEGLIEEFLV"
     gene            5574..8780
                     /gene="cas3f"
                     /locus_tag="PLB25_06455"
     CDS             5574..8780
                     /gene="cas3f"
                     /locus_tag="PLB25_06455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated helicase Cas3f"
                     /protein_id="HOE41254.1"
                     /translation="MHIVLVSACEKRAIKRTRAVLDSYALRTGPSSWGTPITQEALKE
                     LHTALKRSATRQTSVACYKNEGLRRMKLLWMVGSRTSFGADGHFSCGTQQARKPVVVI
                     PDWLRVASVLAKASGKIHDIGKASIRFQRKLRYQADGADRVRHEWLSVKLIQAMRING
                     RDWEKAWKSVKTNKANETDVTLGNRKFSGNDVSAVTGPLEAIDLLVMTHHKLLGTGEL
                     RNERARPTSSEHVRGKVQDFKDEQYTPAGSIGDPIWKNYWTAESRLLQMGESKTPNYW
                     RGVSLFARTALIFADHTVSAINRIDPVTPANPNTIYANTYRSAPSQTGSLNQTLDWHL
                     DNVGSRAAELMVKMHGMALPGLTEESVESICQRSPGKYEWQNTAANALQTAVRSLPDE
                     IKGQVPVLVFNIAGTGSGKTRMNVRAACVLRDGQSAPRLAIALNLRSLTLQTGEALRS
                     QLKIGEDELATVIGDETVRKLFRKTQQGNFVDADENAPESDFICDGEERALPEWLAPL
                     FVQSSNKQRLILSSPLLVSTIDYLIAAGEPGRQGHHVKAFLRVMSSDLVLDEIDGYDP
                     MALVAVLRLIQVAALAGRNVICSSATLAMPVAHAVAAAFRSGVAMREAMEGRACGFLC
                     AQIDDVLPPSVTQMTTETDATTQAFETSYSQRLDAIKQALSARCPMRIAMLKKLNPLE
                     ITTKGWLNAATECCWHLHAWQGWQFHETGIKISFGLVRVANIRTAVLLSRHLSNSLPG
                     GGLTVKIACYHSADFLISRFHKERRLDHLLSRGAGNAGIEADAEIQRDVALAAAKGCT
                     SLMFIVVATPVEEIGRDHDFDWAVIEPSSAQSVVQTAGRVNRHRMQPVTLPNIAILQF
                     NLRHCRNVENGESEKKAFVYPGLELIDDAENSTHGTHDMAELFAWSHSDQVTIDARLR
                     FDHDASRLAACDDKSLTHSLNRFFGAQSEQRKSLFDNENFACQLIGQTVYDASPLRSL
                     SDQKREFFFTEIDASQQPVLRQKLPDGQTPIEQLQRSPSERAGRDENDWLTSSFEAML
                     QMCEEFDVKPMLGTTLVISSYSKETEIVLDESYGACTR"
     gene            9333..>9565
                     /locus_tag="PLB25_06460"
     CDS             9333..>9565
                     /locus_tag="PLB25_06460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41255.1"
                     /translation="MGIKNANGQIITTMLQIVNPQDAVAFGRLMEFLSPTPPWNRSLW
                     SIGLVLGLQELCEASRGSAPKPAEFVQSVSAAYS"
BASE COUNT         2204 a         2656 c         2588 g         2117 t
ORIGIN      
        1 ttagcggctc acaattctta aagcccccgg ctttgccggg ggatggttac tggcactctg
       61 aaggtggcta cttcttctca accacctgca cggtgggtca caaaacgcag tcacccctac
      121 ttacgcacat gaacttctca accacctgca cggtgggtca cttgtggtac gccctcgaca
      181 agtggcacgc agacttctca accacctgca cggtgggtca cgctgcgtca accgtcagca
      241 gggtgaccgc tgtcttctca accacctgca cggtgggtca cggcgaccgc attgcgcagg
      301 cgatgattat ccccttctca accacctgca cggtgggtca cgatttgtcg gcttttgaat
      361 caatcctttt gtgcttctca accacctgca cggtgggtca cttgatgccg actttctgcc
      421 tgttcgcaat gtccttctca accacctgca cggtgggtca caatcaccag taccgaacgc
      481 aaagtctgtc agtcttctca accacctgca cggtgggtca cgcttcggtc cacggtgcgg
      541 caaatccgct gttcttctca accacctgca cggtgggtca caatcaccag taccgaacgc
      601 aaagtctgtc agtcttctca accacctgca cggtgggtca cgcttcggtc cacggtgcgg
      661 caaatccgct gttcttctca accacctgca cggtgggtca cccacacaag agcaaatcac
      721 tgcggcactg gaacttctca accacctgca cggtgggtca ctcaagcagc catgtgaagc
      781 tgatcgacaa atacttctca accacctgca cggtgggtca ctagcggaaa aaaaagaaat
      841 attatattaa atcaacaagt tacggttact taagccctga ctagttcgat tactgaagaa
      901 tctctaactt gttaatttgc aacagatttt tcacacagtg atttaacgaa accaaaaaga
      961 aggtcaagaa cctcttcttc ctgtagtttt tcaatcaaca tctggcgaaa gtcccgtgaa
     1021 ctctcgcctt gttttccaca gtgaaatgcc atgggcataa cgaaggcatc cttgatgagg
     1081 tcggcaacat cgaaaacgag cgcaccgcga cgcgtctttc catgaagcaa tggtaaggaa
     1141 aaacttattc ccaacccgtt cagggcaaca gccgcatagc cataggcaat gtaattgccg
     1201 tggtctaaaa agccattgat gatgtctgct gtggactgac ttgagccttt tccttcttcc
     1261 cggctgaatt caatcctgta ggtattggca agcaccgcat aaagactttt ggcaaaccgg
     1321 gcctcgctca gcagcaacgc ttccgtgctg gctccggcag ccacttcatg acgcagtttg
     1381 tccagaagtg gcagtggcac cgcaagtcct cgctgcagca gttctggcat ctttgcccat
     1441 gaagtctgga taatgtcgca gcgcttgagg agcaagttcc tggccaatcc caggcggcgc
     1501 tcctcgtcga gccaggcacg catccactcc tgcatgtatt ccgttggtcg gtattcgctc
     1561 aggggcgtga ggaaggcgta atccaccgtc gagaacaagg gcgatccacc cgttccgcaa
     1621 aaaccaacaa tcacgttgga ctcggctagc cgtctggcag cggcatctgt aatggatgaa
     1681 cccttgccga gaagaatgaa cgccgtattg cgctcgggaa cattgaaata gcgctcaatc
     1741 agattgccgt cctcagtgat gtaaacaatc cggccatctt tctgaatgac gcgcacatgc
     1801 tcaaggtaaa acacgttggc gcgcttggaa agcatgactg cacgaggctt cacctggggc
     1861 cgtggcgctt tcttttccgt catggcacat ccggcaaagc aaatgcacgc ttcgaggaag
     1921 acagaccgta ggaatttggc ctgaactcgc caggtccagc cgctgccatg ctttgcacaa
     1981 aaagagagaa tttctgctgg ttgctctgac tgtcgctgat caggtaaacc agcccgtctt
     2041 tttgcgcctg agccgagcga cgctgcccca gcgttggttg gcgcgcttgg gcattacggt
     2101 cagactttgc cgaaggaatc cggtatcgcc ggtaggatac ccaggcaccc gcgtagtccg
     2161 caggaactgc cgcaggatag cccagccgag tgtagtcacg aacaaaccag tgcgactgga
     2221 tcgccccagc caaaaagtcg agttctgttc gatcacgcgc aaaaacccga acgcttgaaa
     2281 atccatgcga tggcagcgca atcgcgaacg tctcgggctt gctctggaaa accccgtgca
     2341 ggatgcgcat gagcctggcc agcagaatcg gcttgggcga ggtttcttct gccctcgaca
     2401 gaagatcaat ctgtgtgtat tgcagcatca gacatctccg ccagaataaa caccaccacg
     2461 aattatgcag gccaacagaa actggccgtc atcgctcgcg gggtcgagtg agccaacatg
     2521 ccgtaagatg tcaaaaccgg aggttttctt actgcgaaaa aacatctgcg catccaggct
     2581 tgcaccgttg ggctccaccg caatcgccac gccacgttgc gcgaaggccg gataccaggt
     2641 gtcgatggtg cgcagcgcat tgccgatctt ctggtcgcgc aatgcggcct ggcccagaaa
     2701 aaggatgccg tgcttgtcct ggtcttgcgg ggcattgccc aactgataga gcgagcgggc
     2761 gaaacctttt tccttggctt ccaaataatt ctgtgatggg aacacctcta gcggaccctg
     2821 aacgccgaat ttcagctcgg cacaaacctt gatcaaggca ttacgctggc cgcaaaaacc
     2881 ttccttgata actttggcca gatcgctctc tctttcggtc ggcatgtcaa agtggttgag
     2941 cggaaccgct agcgcatcaa actgtagctg gctgctgccg atggaggctg ttacgttgat
     3001 ggattcggaa atcatccggt tgcgccagag ccaccggcca ttggcgatgt tgcgggcgta
     3061 gcgcaggctg agcaggtcta gagcgtcccc ttgcttggcc cgctcaataa attcagacag
     3121 ggtttccttg aacttattga tgtcgtccat cgtgtcgctt ttgctgggtg cgcaggcgaa
     3181 cagggcaccc gacagatctg caaagcgcac atcgaagcga acttccaggg cgctggcttc
     3241 gggagccagc tttgcactgt cagtctgttg gatgttgctg acttcgcggc ctttggcacc
     3301 cgctaccgag gcagcggcat catcggattt gctttcattt acgttctggg ttccgcgaat
     3361 tccatggcgc atcacataca gcggggcgcg ttcgccaccg ggcatcaggc tgtagaacat
     3421 ggcatcagac agaatcatgc cgcgctgaag cgacagaact ccggggagtt ttttgaatgg
     3481 aacagcggat ttctggatag cagtggtcat gaatcaactc ctttgagata acaaaaaaac
     3541 atcgggatgg ggccaagcac aggcccacat caaacttttc aactcggaag cggctttgcg
     3601 gagggacacg tactgaacaa gacccgtcag gggttcggca aaagcatgtt caactccttc
     3661 gcgaacaccg cctttgtgct tgaactcggt gagcgtggcg tacccgagcg ttgtggcggc
     3721 aagccagggc ggatgagcat caagaagctg tgggtttgca gcgagtttct tgatcagtac
     3781 agccgcacgg ctttctcctg gctgctccag ttcgattgat ttgtcgcgaa gcacaaagcc
     3841 gctgggcacg cgcttgaacg cggcctgcaa cgtttcttct gtttcaatct gcccatgact
     3901 gagcacggtg ccaccggaaa atttccccct ttccagcatc ttgtggacgg catccacgtc
     3961 ctgcaaatca tcgaatttga taactacgct cacacgcaaa tgtgcactca gcgtgggctg
     4021 aagcgacagg gcttgcttgt tggtggagct gtagtcgctg ccatcaataa acatggcacc
     4081 ccggcgctgt tgcccatgaa gtcggccata aataaattgc cccagttcct gccggtcatg
     4141 gtgaatcaga aaaaatccca aatcccgagc gcctgcctcg cgtgcgacct ggtgggcaaa
     4201 cagggcgcag gccatcagag gcggaccacc ggcaacaagg tttgaggtca gcaggttggc
     4261 accatgaacc cgcatggaag gaatcacgag gtaggtcata caaggaactc ctcgatcagg
     4321 ccctcaatgc ttttgatgcc gaaaacatcc aggctcaaca agcgttttgg tccatttttg
     4381 gtttgaactt caaatttgcc gatcgcattg gccagccaca cagccgccag ggatgcccag
     4441 tcacgcccgc gcagggactc atccacccag gcggcgttca cgttgtggtg cagcttttga
     4501 gtgaaataca tgtgcttgcc tgtggtatct gcgggcagca tttcaagatg ctggtccagt
     4561 acacgcctga tgtcatcgac ctgatccata acatcgtcca cgaactcggc aagaatggat
     4621 ttctcttcct cgcggctttc catggcggtc tggcgaacgt cgtcctcggt atttgctttc
     4681 agaaaatccg cgtacttgcg caccagtttc agggaaatgt tcaggtcaac gcccttgtgg
     4741 tgcatggcca gcgcctgccg gagactacct ttggcctggg gcgcacccat gtacatcggc
     4801 tgctgcatct cccgcaccaa cccgcccacg ttttgagggt tagacccacc gattcccagt
     4861 tgagtcaact gaaccgagcg tgtcgccaat cgcgcgatga cggcctttgg gtttttcttc
     4921 agtgcctttt ctttttcctt ttgccaatct tcctttccga gcttatgcgc ttcgttggaa
     4981 gcagctacat gggcgcgcat gatttggcac aggccgcccg ccgtcaatgg cgtgaccgag
     5041 cggtaactga cagcgcccgt ctcatcggta tagggaagga gcaactggcg cagtcgaggg
     5101 tccaggaatg catcgcaccc atcttccaag cgcccttggg tgcagcgtgc gagggcatcg
     5161 ccaacagcca gaagctcatc aggcgcaagg tctggcgcgt cttccttttc agcggacttt
     5221 gcaccatcct tatcagcttt cttcttcgga ataatgccac tggcatccat cctggcctgc
     5281 ttagcatgtt tgaaatgagt ggcgatgcgt cccgagtttt tggtgtagtc ggtcatgcat
     5341 gaaatgccct ctttcagcag ggttcttgca ctcacgtagg gcaaattcaa ggcatcagcg
     5401 gcatcaatcc gaatgccccc tgcaggcgca tttgaattga ccgtcttaaa agaaaaatgc
     5461 acaaatcccc ggaaagaact ttcgtactct tcaatttttt cattcattta ttatccataa
     5521 tagactgatg ttaagataca tatcttactc gtttgttttg agagaagcaa tacatgcaca
     5581 tcgtcttagt gtctgcctgc gaaaaaagag ccatcaagcg aacaagagcc gtcctcgata
     5641 gctacgcact gcggacaggg ccgtcaagct ggggaacgcc gatcacccag gaagcgctga
     5701 aggagttgca tacagctttg aagcggtcgg ctacccgaca gacctcggtg gcgtgctaca
     5761 aaaatgaagg actgcggcgc atgaagctgc tgtggatggt gggctccagg accagctttg
     5821 gagccgatgg tcatttctcg tgcggcaccc agcaagcccg aaagccggtc gtagtgattc
     5881 cagattggct cagggtcgcc agcgtgctgg ctaaggcatc tggaaaaatt catgacatcg
     5941 gcaaggccag cattcggttt cagaggaaat tgcgctatca ggccgatggg gctgaccgtg
     6001 tacgccatga gtggctctca gtcaaattga ttcaggcgat gcgcatcaac ggacgcgatt
     6061 gggagaaggc ctggaaatcg gttaaaacca acaaagccaa cgaaacggat gtcactctgg
     6121 ggaaccgaaa gttttctggt aatgatgtca gcgcagtgac tggcccgctg gaggccattg
     6181 atctgctggt gatgacccat cacaagcttc taggaactgg ggaattgcgc aacgagcgcg
     6241 cccgccctac cagcagtgag catgtgcggg gaaaagtgca ggacttcaag gatgaacagt
     6301 acacccctgc cgggtcaatt ggcgatccaa tctggaaaaa ctactggaca gcggaaagcc
     6361 gcctgctgca aatgggcgaa agcaagacac cgaattactg gcgtggcgtc agcctgttcg
     6421 cccggactgc gctgattttt gccgaccaca cggtttccgc catcaatcgg atcgaccccg
     6481 taacgccagc gaaccccaac accatttacg ccaacaccta ccgtagcgcg cccagccaaa
     6541 ctggttcgtt aaaccaaacc ctggattggc atctggacaa cgtgggcagt cgggccgctg
     6601 agctgatggt caaaatgcac ggcatggcat tgccggggct tacggaagaa tcagtcgaat
     6661 ccatctgcca gcgctcgcca gggaaatacg aatggcaaaa cacggcggca aatgccctcc
     6721 agaccgctgt gcgctcgcta ccggatgaaa tcaagggcca ggttcccgtg ctggttttca
     6781 acattgcagg caccggcagc ggaaagacaa gaatgaatgt gcgtgccgcc tgcgtgctca
     6841 gggacggcca gagcgcgccc agactggcca ttgccttgaa tctgcgctcc ctcacgctgc
     6901 aaacaggcga ggcactgcgc agccagctca aaatcggcga ggacgagttg gcaaccgtca
     6961 tcggggacga aactgtcaga aaactctttc gcaagacaca gcaaggaaat ttcgttgatg
     7021 cggatgagaa tgcgcccgaa tcggacttta tctgcgatgg cgaagaacgc gcgttgccag
     7081 agtggctggc ccctttgttt gtgcagtcct cgaacaagca gcggctcatc ctctcgtccc
     7141 cgctgctggt gtccaccatc gactacctga tcgcggctgg agagccgggt cggcagggtc
     7201 atcatgtcaa agctttcttg cgcgtgatga gcagtgatct ggttctcgat gagattgatg
     7261 gctatgaccc gatggcactg gtagccgttc tgcgcctgat ccaggtggct gccctggcgg
     7321 ggcgcaatgt catttgctcg tcggcaactc tggccatgcc ggtggcccat gcggtggcgg
     7381 cggcttttcg cagcggcgtg gccatgcgtg aagccatgga aggcagggcg tgcggttttc
     7441 tctgcgcaca aatcgatgat gtcctgccgc cttcggtcac acagatgacg acggaaaccg
     7501 acgcaactac gcaggcattt gagaccagtt acagccaacg gcttgacgcg atcaaacagg
     7561 cgctcagtgc tcgttgcccg atgcgcattg ccatgctgaa aaagttgaat cccctcgaaa
     7621 tcactaccaa gggctggctc aacgccgcga ctgaatgctg ttggcacctg cacgcctggc
     7681 agggatggca atttcatgag actggaatca aaatctcatt tggactggtt cgggttgcca
     7741 atattcgcac tgccgtcttg ttgtcccgcc atctcagcaa ctcactgccg ggtggtggct
     7801 tgaccgtcaa gatcgcctgc tatcactcgg cagatttcct gatttccaga ttccataaag
     7861 agcgcagact ggatcatctg ttgtccaggg gcgcgggaaa tgcagggatt gaggcggatg
     7921 ctgaaatcca gcgcgatgta gcccttgctg cagccaaagg ctgcacatcg ctcatgttca
     7981 tcgtagtcgc gacacccgtc gaggaaattg gccgagacca tgattttgac tgggcagtga
     8041 ttgagccttc cagcgctcaa tcggtggtgc aaacggcggg ccgggtgaac cgccaccgga
     8101 tgcagccggt gacgttgccc aatatcgcca tcctgcaatt caacctgcgc cactgccgca
     8161 acgtcgaaaa tggtgagagc gaaaagaaag cgtttgttta ccctggtctt gagttgatag
     8221 acgatgctga aaacagcacg catggaaccc acgacatggc agagttgttc gcctggagcc
     8281 attcggatca ggtcaccatt gatgcaaggc tgcgttttga ccatgacgca agccggctgg
     8341 ccgcgtgtga cgataaatcg ctgacccact cgctgaacag gtttttcggc gcacagagcg
     8401 agcagcgcaa aagcctgttt gacaatgaaa acttcgcgtg ccagctgatc ggccagaccg
     8461 tgtatgacgc cagtcctttg cgaagcctgt cagatcagaa gcgggagttt ttcttcaccg
     8521 agattgacgc ctcacagcag ccggtgctca ggcagaagct gcccgatggc caaactccaa
     8581 tagagcagct ccaacgcagc ccatccgagc gtgcaggaag ggatgaaaat gactggctga
     8641 cgagcagctt tgaggccatg ctgcagatgt gcgaagagtt cgacgtgaaa cccatgctgg
     8701 gaacaactct cgtcatctcc agctacagta aagaaacgga gatcgtgcta gatgagtcgt
     8761 atggagcatg cacccgatga tccaccgtgc aggtggttga gaaaataatc gactggtgtg
     8821 cattgccaac gttatccacc gtgcaggtgg tgttgagatt tttatattca ctctgcttct
     8881 gttgttggag tctgaggtgc attggagcag taaaagcctt taagctgtca agttaaacag
     8941 ctaaatgaat ctggcagtca gtccgatgct ttcttgatcc attaggtaac ggcaaagatc
     9001 gcaactctcc cttgcccaca gactctggac tttggggagc aatggaacgg aaaaccgggt
     9061 taagtggcca atttaagagc caaataggtt ttagccaatt tacatcggta aattttcgct
     9121 accccaagtg gatgcgattt ttaagggtgc tgccactggc tccaatgcag cgttatgcca
     9181 ttcacttgcc gggtctgcct aaagcgcgga cgtatcttgc ctgcgcacaa aaaaaagccc
     9241 cgcattgcag ggccttttaa agggaggggc atggagccga gcggcgtcag gtgagagcac
     9301 tttctgcgtt tcgggtcggg tccccttaga ctgtgggcat caaaaatgcc aatggacaaa
     9361 tcataacaac tatgctccaa atcgtcaatc ctcaagacgc cgtggccttt ggtcggctca
     9421 tggaattcct gtcgccaacg cccccctgga accgttcctt gtggagcatc ggcttggtgc
     9481 ttggcctcca agagctatgc gaagcaagta ggggttccgc gcccaaaccg gcggaatttg
     9541 tccaatccgt tagtgctgcg tattc
//
LOCUS       DAOSLL010000107         9497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6931_length_9497_cov_4.677717, whole genome shotgun sequence.
ACCESSION   DAOSLL010000107 DAOSLL010000000
VERSION     DAOSLL010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6931_length_9497_cov_4.677717"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..2339)
                     /locus_tag="PLB25_06465"
     CDS             complement(24..2339)
                     /locus_tag="PLB25_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41256.1"
                     /translation="MANKPLLLALAALVLGSGGAWATTSINHQFSPSVINQGDNSLYT
                     ITITNDSTTPLTAASATIFLDNTTAAPNTAGGRVTITSGTVLSNTCGFSGVTADATDN
                     KIILTGGTIAAGTVGTPSQCTFSLNVTSILVGTYHAVFPANTTPSASVTGYESTENTV
                     QVRNGTAADITLQVNGLSAPTGVKSYSPSPAIAGDPTTLTVTLTNPNGSATMPLTSFV
                     DALPSGMQVASAPAGSVNCSGTGAVNGSFAPTTGATTLTLTGGTIGQGGTCVLAVKVI
                     VPTVTGTSQVLNNSLGAGAIGNTRGLSSPAFNTDLTVNSPIGVSKSFNPTTVPAGQPS
                     LMTLTLSNNSTTTLLPVTSFLDEFSASTLTILDVAHGATTEPSVTCSGTGGVPGTLTD
                     SADVALTNGSTSIKWVGGTVAKSSTCVITAYVTSTLDGGHTNTVAANAVVNQVENHSS
                     PSANASLTINGQLTVDKTVTLSSVAPGQWTQFTVTIRNWSGGQIDNVLFSDVLPLVAA
                     TLPAVGGQMVLQGANPVSNVGCIGGTWTGVDGEATLAWSGGSIAAGSGATPGICTIVF
                     KARLPSSATTGVMFANQIPINGASGICNGAGCSVGNGNTVGNPAASPAVNVTTVDSVA
                     MAKSFSPASIAQGGLSTMTLTLRNRTLSPLSAINLTDNFPAGLTLATNPAAVNNCGGA
                     LTAFPDDNKLILTGGSFLARPDASTDRTCTITVKVTSTTLGGAPDNRNTIATSDLSTS
                     AGTIPASVSATLTVATGLTGTKNLSVNNVYT"
     gene            complement(2705..2992)
                     /locus_tag="PLB25_06470"
     CDS             complement(2705..2992)
                     /locus_tag="PLB25_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011408623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOE41257.1"
                     /translation="MAPQRCQSWEPAVRTNACRSAEEVAKMIRSDLEGIVAWTQTRQT
                     NGFLEAVNGLFQASKRRARGFTRLSTIRTVIFLIAGKLDFQAINPHAGLRT"
     gene            complement(3161..3952)
                     /gene="cobA"
                     /locus_tag="PLB25_06475"
     CDS             complement(3161..3952)
                     /gene="cobA"
                     /locus_tag="PLB25_06475"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOE41258.1"
                     /translation="MNSPLPPSATPTGCVYLVGAGPGDPELLTLRAVRLLQRADVVVY
                     DNLVSAGVLDFVPATAKRIYAGKRRNEHTMRQAQINALLVKLALAGKQVVRLKGGDPF
                     IFGRGGEELQALAANGVKFEVIPGVTAASGVSSYAGIPLTHRDHAQTCIFVTGHLKDG
                     TADLDWPSLVRANQTVVIYMGLTGLPEICRQLIAHGAPATFPIAVVQDGSIKTQKVVT
                     GSLADIAGQVARQQLKSPCLIIIGNVVKLHSELAWFKPAETAPRG"
     gene            complement(3949..4326)
                     /locus_tag="PLB25_06480"
     CDS             complement(3949..4326)
                     /locus_tag="PLB25_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41259.1"
                     /translation="MFTLTSAAAQQTQPAADASNRRHLALWLAVKVDAKGGLLHGLGF
                     DKPADADLMLILADVNIVINTDKLDMLDQPEQDCLDILAGELNVIFADGLLVKSISTG
                     TSPTRGGGDRGGAGYACQAGGCH"
     gene            complement(4340..4624)
                     /locus_tag="PLB25_06485"
     CDS             complement(4340..4624)
                     /locus_tag="PLB25_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41260.1"
                     /translation="MIAIVKDWRKLKFSPYTCPQIFRSWLPRRPLFDAPCPPMANKSE
                     PTAFSAPLILCDLWPDGGATYAFFPAGSPHRQDKRQAGHWQTGARIKPGF"
     gene            4974..6224
                     /locus_tag="PLB25_06490"
     CDS             4974..6224
                     /locus_tag="PLB25_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE41261.1"
                     /translation="MLKLSKLAMALAFAVAGNAAMAGEVEVLHWWTSGGEAKSVGELK
                     KIMQSKGNTWKDFAVAGGGGDNAMTVLKSRVVAGNPPAAAQIKGPGIQEWAVEGLLAD
                     LTTVATAEKWDALLPGVVADVMKYKGKYVAAPVNVHRVNWMWANTAVLKKAGVAGAPK
                     TWDEFFAAADKVKKAGLIAVAHGGQNWQDFTTFETVVLGVGGAKFFQEALVKLDPKAL
                     TSPTMTKSLETLRKVKGYTDAAAPGRDWNLATAMVIQEKAAFQFMGDWAKGEFTAAGK
                     VPGKDYICAAAPGTAGSFTFNVDSFAMFKLKDAAAQKAQADLAAAIMGVEFQEIFNLN
                     KGSIPVRLNMDMAKFDDCAKLASKDFVAASKSGGLLPSIAHGMAVSPAAEGAIKDAVS
                     QFWNDDKMSVADGMKSIAKAAAAK"
     gene            6617..7495
                     /locus_tag="PLB25_06495"
     CDS             6617..7495
                     /locus_tag="PLB25_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE41262.1"
                     /translation="MKSFENVLPKLVIAPGFVLGFAFIYGLMIWNGLLSVTNSRMLPN
                     YDDFVGLEQYVRLWEMDRWYVALKNLGIFSILYVGGSMLLGMVLAIFLDQKIRFEGVI
                     RTIYLYPMALSFIVTGTAWKWILNPSLGLEKLMHDIGWASFSFDWLVQSETAIYCVVI
                     AGIWQSAGFAMALFLAGLRGIDDSIIKAAQIDGASLPRIYWRIILPVLRPVVFSTILV
                     LSHLSIKAFDLVMALTAGGPGYASDVPATFMFTMSFTRGQVGLGAASATMMLAMVAAI
                     VVPYLYSELRSKPHER"
     gene            7510..8364
                     /locus_tag="PLB25_06500"
     CDS             7510..8364
                     /locus_tag="PLB25_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE41263.1"
                     /translation="MNAKTLSRFAVYAVLLIAAFFFLAPLYVMLATSFKDAEQIRSGN
                     LLSLPTSLNFESWTLAWSTACTGIDCRGLKPYFWNSVMMAVPAVLISTGWGAINGYVL
                     SMWKFKGSDLLFGFILFGVFMPFQVVLLPMSQVLGFLGLSSSIGGLVLVHCLAGMAGT
                     TLFFRNYYTAIPRELVNAARMDGAGFWRIFYRIVIPMSTPILMVTLIWQFTNIWNDFL
                     FGVAFSGADRKPITVGLNNMANTTSSVKSYNVDMAAAIIAGLPTMLVYVLAGQYFVKG
                     LTAGAVKG"
     gene            8379..9449
                     /locus_tag="PLB25_06505"
     CDS             8379..9449
                     /locus_tag="PLB25_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE41264.1"
                     /translation="MAASLQIAGIRKVYGKGDKSVEVLKKIEIDVAPGEFLILVGPSG
                     CGKSTLLNIIAGLEDPTEGELRIAGKNVIGVAPAQRDIAMVFQSYALYPTMSVADNIG
                     FALEMRKVPLEVRNKRIHEVAAMLQIEHLLDRRPAQLSGGQRQRVAMGRALARDPQLF
                     LFDEPLSNLDAKLRVEMRAEIKRLHQVSGITSVYVTHDQIEAMTLGSRIAVMKDGILQ
                     QIGTPDDIYRRPANTYVAGFIGSPTMNFITGSASGTGATGQFSFEGGALQLPCPAVGK
                     VTLGQRPEHIHIEAQATWRGEVTLVEPTGADTYVVVKTGSGLITVRTPPSTTVKVGEN
                     IGLSVSSQHNNWFDAQTGLRLA"
BASE COUNT         1917 a         2623 c         2849 g         2108 t
ORIGIN      
        1 tcatggcatt tcgcaaaatc aagttaggtg taaacattat ttacggataa gttcttagtg
       61 ccagtcaacc ctgtagcgac cgtcaaagtt gctgaaaccg aagctggaat ggtacctgcc
      121 gatgtggaca agtcggaggt ggcgatcgtg tttcggttat ccggcgcgcc gcccaaagtg
      181 gtgctggtca ctttaaccgt gatggtgcag gtcctatcag tcgaggcatc cgggcgcgcc
      241 aaaaaactgc caccagtcaa aatcagcttg ttgtcatcag gaaacgccgt tagcgctccg
      301 ccgcaattgt tcaccgcagc cgggttggta gccaaggtca acccggccgg gaaattgtca
      361 gtcaaattga tcgcactcaa cggactcagt gtgcggttac gcaaggtcag tgtcatggtt
      421 gacagcccgc cctgagcgat cgaagctgga ctaaatgact ttgccatcgc caccgaatca
      481 accgtggtca cgttgaccgc ggggctggct gcggggtttc cgaccgtgtt gccattgccc
      541 acgctacagc ctgctccatt gcaaatgccg gaggcaccat tgatcggtat ctggttagca
      601 aacatcacgc ccgtggttgc gctgctgggt aggcgggctt taaacacaat agtgcagatg
      661 cccggtgtag cgcctgaacc ggccgcgata gagccacctg accaggccag cgtggcttca
      721 ccgtcaacac cggtccacgt gcctccaata caacccacgt tgctcaccgg atttgcacct
      781 tgcagcacca tttggccacc cacggccggg agggtcgcag ccaccagcgg cagcacatct
      841 gaaaagagaa cgttgtcaat ttgaccaccc gaccagtttc taatcgtcac ggtaaattgc
      901 gtccactggc ctggcgcgac tgagctcaac gtgactgtct tgtccacggt gagctggcca
      961 ttgattgtca aactggcgtt ggcagaggga ctactgtggt tctctacctg gttgaccaca
     1021 gcatttgctg ccactgtgtt ggtatgacca ccgtccagtg ttgatgtcac ataagctgtg
     1081 atcacacagg tgctggactt ggccaccgtg cctccgaccc atttgatgct ggtgctgccg
     1141 ttggtcaaag caacatcggc actatcggtc aacgtgcctg gcacgccacc agtgccactg
     1201 catgtcacgg aaggttcggt cgtcgcgcca tgcgcgacat ccaggattgt caacgtcgat
     1261 gccgaaaatt cgtccagaaa actcgtcact ggcagcagag tggtggtact gttgttggag
     1321 agggtgaggg tcatcagcga cggctgaccg gctggcacgg tagtcgggtt gaacgatttg
     1381 ctgacaccga tgggcgaatt gactgtgaga tcggtattga aggccgggct gctcaggccc
     1441 cgggtgttgc caatggcacc ggcacccagg ctgttgttga gaacttgcga ggtacccgtg
     1501 accgttggca cgatcacttt gaccgccagc acacacgtgc cgccctgccc gatcgtgccg
     1561 ccagtcaggg tcaacgtggt ggcaccagtc gtcggagcaa agctgccatt gactgccccg
     1621 gttccgctgc aattcaccga accagcaggc gcgctggcca cctgcatgcc ggacggcaga
     1681 gcgtctacaa agcttgtgag cggcatcgtt gcgctaccgt tggggttggt cagcgtgacg
     1741 gtgagcgtgg tcgggtcgcc ggcaatggcc ggcgatggcg aataactctt gacccctgtg
     1801 ggtgcgctta gcccgttgac ctgcagggtg atgtcagcag ccgtgccgtt tcgcacctgt
     1861 accgtgtttt cggtggactc ataacccgtc actgaggcgc ttggcgtggt gttggccgga
     1921 aacacggcgt ggtaggtgcc taccaggatt gaggtgacgt taagcgaaaa cgtgcattgc
     1981 gacggcgtgc caactgtgcc ggccgcaatg gtgccaccgg tcaggatgat cttgttgtct
     2041 gttgcatcgg ccgtaacacc gctgaatccg caggtgttgc tcagcaccgt gccactggtg
     2101 atggtgacac gccccccagc agtgttgggc gcagccgttg tgttatcgag aaagatcgtg
     2161 gcgcttgcag cggttaacgg cgtggtggaa tcattggtaa tcgtaatggt gtagagcgag
     2221 ttgtcaccct gattgatcac cgacgggctg aactggtggt taatcgaggt agtcgcccag
     2281 gccccacccg atcctaaaac cagcgccgcc agcgccagca gcaagggctt gtttgccaac
     2341 ccgctacccc agcgttccag gactgacttg aaacctgacc aaatcgaacc gactattgca
     2401 agaccgaact ggtttggcgc ggcaagggca aatgactcgt ttttcactgt aactccaatc
     2461 agcacagcgc aagctgttga ggaggacttc agtcgggcgt gctatggctt gattgtcgtc
     2521 ggtcggaaga tgtcactcgc atcagccttc gcgaattgcg tctttttttg cattggggtc
     2581 ttgatgacaa ataacgtttg ctttgagccc atttcagtag gcatgcaagt tgttgatttc
     2641 attgattaag ccttgcctgc tgaattgcgc gcttcacgcc ttctataaac ggctccaatg
     2701 aatttcaagt gcgtagccca gcgtgagggt tgatggcttg gaagtcgagt tttccggcga
     2761 tcaagaagat aaccgtcctg atagtggaga gacgtgtgaa gccacgagca cggcgtttgg
     2821 atgcttggaa caagccgttg acagcttcca ggaatccatt ggtttggcgt gtctgtgtcc
     2881 acgccacgat gccctccaaa tcagatcgaa tcatcttggc gacctcttct gcgctgcggc
     2941 aggcattggt tcgaacggct ggctcccaac tctggcagcg ctgcggtgcc attccttact
     3001 cggctcgccc ggctttcaat caaggccacc gagtggcatc gactatatgc aaaaacaata
     3061 gcatgttacg catatggcac gggggctaga ggctgatttg gtgccaattt tgcaggctga
     3121 ccaaagagcc cgtttacagg caacaaaagc ctcaagccgg tcagcctctg ggtgccgttt
     3181 cggctggctt gaaccaggcc agttcgctgt gcaacttcac cacgttgccg atgatgatca
     3241 ggcagggcga cttgagctgt tgccgggcca cttgcccggc gatgtcggcc aagctgcctg
     3301 tgaccacttt ttgcgtcttg atgctgccat cctgaaccac cgcaattgga aatgtggcag
     3361 gtgcgccgtg cgcgatcagt tgacggcaaa tttctggcaa tccggtcaga cccatgtaaa
     3421 tcacgaccgt ctggttggct ctgaccagac tgggccagtc caggtctgcc gtgccgtctt
     3481 tcaggtggcc ggtgacaaaa atgcaggttt gcgcgtggtc gcggtgcgtc aggggaatgc
     3541 cggcgtaact tgagacaccc gaggcggccg tgacaccggg tatgacttca aacttcacgc
     3601 cgttggcggc cagggcttga agttcttcgc ccccgcgccc gaagatgaag gggtcacccc
     3661 ctttcagccg caccacctgt ttgccagcca gggccagttt gaccagtagc gcgttgatct
     3721 gtgcctggcg catggtgtgc tcgttgcgcc gcttgccggc atagattcgc tttgccgtgg
     3781 ctggcacaaa gtccaacacc ccggccgata ccagattgtc ataaaccacc acatctgcgc
     3841 gttgcaacag gcgcaccgca cgcaaggtca acagttcagg gtcgccaggg ccagcaccca
     3901 ccaggtaaac gcagccggtc ggcgttgccg agggtggcaa aggtgaattc aatggcaacc
     3961 tccagcttgg caagcgtagc cagcgccacc gcggtcaccg ccaccgcggg tggggctggt
     4021 accggtgctg atgctcttga ccagcaggcc atcagcgaag atgacattga gttcaccagc
     4081 cagtatgtcg agacaatcct gctcgggttg gtcgagcatg tccagtttgt cggtgttgat
     4141 cacaatgttg acatctgcaa gaatgagcat caggtccgcg tcggcgggtt tgtcaaagcc
     4201 caagccgtgc agcagaccgc ctttggcatc gactttgact gccagccaca aagccagatg
     4261 ccgccggttg ctggcatctg ccgccggttg agtttgctgc gctgctgcgc tggtcaacgt
     4321 gaacatgctt gggctccagt cagaaaccag gtttaattct cgccccggtt tgccagtggc
     4381 cagcctgacg cttgtcttgg cggtgcgggc tacccgccgg gaagaaggcg taggtcgcgc
     4441 cgccgtctgg ccaaaggtca cacaaaatca atggagcaga gaaggctgtt ggctcacttt
     4501 tgttggccat cggcgggcaa ggcgcatcaa acagcggcct tcgcggcaac caggaccgaa
     4561 aaatctgcgg acaagtgtag ggcgagaatt tcagcttgcg ccagtctttg acgatggcga
     4621 tcaagaggca gttggcgcac cagacctgcg tcttcaccgc gtcctcgcgg gtgcacaggc
     4681 gcgcgcagcg ccggaccgac aggcgactga ggtcctgcgt cggaacattc gcgctgctct
     4741 gaagccattt ccagatcatc gatgtcgctg ttaaccggcc ttgccttgcg tgtccaatgg
     4801 gtgcgccagg gtggtcacgt aagaatgact gaaaaatggc ataagggtaa atcctgattg
     4861 cttttcaggc aaaattaatt aacaattaat taattcgcgc aacatggtgt ctgcacgaat
     4921 caacctgtac ccccaaactg actgattgtg gggtcgtttt tctaggagat gacatgttga
     4981 aactttccaa attggcgatg gctcttgcat ttgcggtggc tggaaacgcg gcgatggctg
     5041 gtgaagttga ggtgctgcat tggtggacct ctggcggtga agccaaatcg gttggcgaac
     5101 tgaaaaaaat aatgcaaagc aagggcaaca cctggaaaga ttttgcggta gccggtggcg
     5161 gcggtgacaa cgccatgacc gtgctcaaga gccgcgtggt tgctggcaat ccgccggctg
     5221 cagcgcaaat caaaggcccg ggcattcagg aatgggcggt cgaaggcctt ttggccgacc
     5281 tgaccacggt cgcaaccgct gaaaagtggg acgcgctgct gcccggtgtg gtggctgacg
     5341 tgatgaagta caagggcaag tatgttgcag cgccggtcaa tgtgcaccgc gtcaactgga
     5401 tgtgggccaa cactgcggtg ctgaaaaagg ctggtgtggc tggcgcgccg aaaacttggg
     5461 acgagttttt tgcagcggct gacaaggtca agaaggccgg tttgatcgcc gtggcgcatg
     5521 gtggccagaa ctggcaggac ttcaccacct ttgagaccgt ggtgctcggt gttggcggcg
     5581 ccaagttttt tcaggaggcc ctggtcaagc ttgaccccaa agccctgacc agcccgacca
     5641 tgaccaagtc gctggaaacc ttgcgcaagg tcaagggtta taccgatgcg gctgctccgg
     5701 ggcgcgactg gaacctggct accgccatgg tgattcagga aaaagccgct ttccagttca
     5761 tgggtgactg ggccaagggt gagttcactg ctgctggcaa agtgccgggc aaggactaca
     5821 tctgcgcggc tgccccaggg actgccggtt ccttcacctt caacgtcgat tcgttcgcca
     5881 tgttcaagct caaagacgca gccgcccaga aggcacaggc tgacctggca gcggccatca
     5941 tgggtgtcga gtttcaggaa atcttcaacc tgaacaaggg ttcgattcca gtgcgcctga
     6001 acatggacat ggccaagttt gacgactgcg ccaaactggc ttccaaagac ttcgtggcag
     6061 cgtccaagtc cggtggcctg ttgccgtcca ttgcccatgg catggcggta agcccagcgg
     6121 ctgaaggtgc catcaaggac gccgtcagcc agttctggaa cgacgacaag atgtcggtgg
     6181 ccgatggcat gaagagcatc gccaaggccg ctgcggcgaa ataagtctcg ttgatcggca
     6241 ccgccgtagt ttgtcatccg ggatttgggt cccggattga tgtttcgggc gttcagtggg
     6301 gcatctgtgg cgtaaacccg cagcaggtct ggggtgcgac ccgccgctca tgtcccccgc
     6361 ctgcggctcc tccttgactt cgctttgggt cacaccccaa ccctgctgcc agatattgcg
     6421 actcaaagaa gcgatgtctg gccctgctga ggtgggtgtt tcgcgtagtg aggtcaagga
     6481 ggagaccgaa agctctgctt tcggtcgggg gacacgaacg aagcgaaaca cccgcctcgc
     6541 caggtcgggc taaagcgcag gtatcccttg ggcgggtcaa caagacacct ggtgtattac
     6601 caaaggcgaa agtctcatga aatcctttga aaacgtatta cccaagctgg tgatcgcacc
     6661 gggttttgtc ctcggctttg cgttcattta tggcttgatg atctggaatg gcctgctgtc
     6721 cgtgaccaat tcccgcatgt tgcccaacta cgacgatttc gttggtcttg agcaatatgt
     6781 acggctgtgg gagatggacc gctggtacgt cgcgttgaaa aatctgggta ttttcagtat
     6841 cttgtatgtc ggtggctcga tgctgctggg catggtgctg gcgattttcc tggatcagaa
     6901 gatccgcttt gagggggtga ttcgcaccat ttatctgtac ccgatggcgc tgtcgttcat
     6961 cgtcaccggc accgcctgga aatggatttt gaaccccagc ttgggcctgg aaaaactgat
     7021 gcatgacatt ggctgggcca gtttcagctt tgactggctg gtgcagagtg agaccgccat
     7081 ctattgcgtg gtgattgctg gtatctggca atcggcgggt tttgccatgg cgctgttcct
     7141 ggcgggcttg cgtggcattg acgacagcat catcaaggcc gcgcagattg acggtgccag
     7201 cctgccgcgc atttattggc gcatcatctt gccggtgctg cgcccggtgg ttttttccac
     7261 cattctggtg ctgtcgcacc tgtcgatcaa ggcctttgac ctggtgatgg cgctcaccgc
     7321 tggcgggccg ggttatgcgt ctgatgtgcc agccaccttc atgttcacca tgagtttcac
     7381 ccgcggccag gttggcctgg gggctgccag cgccaccatg atgctggcca tggtggcggc
     7441 gattgtggtg ccttatcttt acagcgaact gcggtccaag ccgcacgagc gttaatccgg
     7501 agaccagtca tgaacgccaa aacactctcc cgatttgcgg tctatgccgt gctgttgatc
     7561 gctgcctttt tctttctggc accgctgtat gtgatgctgg ccacctcgtt caaggatgcc
     7621 gagcagattc gctctggcaa cctgttgagc ctgccgacct cgctcaactt tgaatcctgg
     7681 acactggcct ggtccaccgc ctgcaccggg atcgattgcc gcgggctcaa gccctatttc
     7741 tggaactcgg tgatgatggc cgtgccggcg gtgctgatct cgaccggctg gggtgccatc
     7801 aatggctatg tgctttccat gtggaagttc aagggcagtg acctgctgtt tggcttcatc
     7861 ctgtttggcg tgttcatgcc gtttcaggtg gtgctgttgc cgatgagcca ggtgcttggg
     7921 tttttgggcc tgagcagttc gattggtggc ctggtgctgg tgcactgtct ggccggcatg
     7981 gctggcacca cgctgttttt cagaaactat tacaccgcca ttccgcgtga actggtgaat
     8041 gcggcgcgca tggacggggc cggtttctgg cgcattttct accgcatcgt gatcccgatg
     8101 tccacgccga ttctgatggt cacgctgatc tggcagttca ccaacatctg gaacgacttt
     8161 ctgtttggtg tggctttcag cggtgccgac agaaagccga tcacggtggg gctcaacaac
     8221 atggccaaca ccaccagcag cgtgaagagt tacaacgtcg atatggccgc tgccatcatc
     8281 gccggactgc caaccatgct ggtctatgtg ctggctggtc aatatttcgt caaaggtctg
     8341 actgccggtg cagtcaaagg ataagaggtt taacaatcat ggcagcttca ctccaaatcg
     8401 caggcattcg caaggtttat ggcaagggcg acaagtcggt cgaagtcctg aagaaaatcg
     8461 aaattgatgt cgctcccggc gaattcctga ttctggtcgg gccctcgggc tgcggcaaat
     8521 caaccctgct caacatcatt gccggcctcg aagacccgac cgaaggcgag ttgcgcatag
     8581 ccggcaaaaa cgtcattggc gtagcaccgg cacagcgcga cattgccatg gtgttccaga
     8641 gctacgcgct gtacccgacc atgagcgtgg ccgacaacat tggttttgcg ctggaaatgc
     8701 gcaaggtgcc gcttgaggtg cgcaacaaac gcatccacga agtggccgcc atgttgcaga
     8761 tcgagcacct gctggaccgt cgtccggcgc agttatccgg tggccagcgc cagcgggttg
     8821 ccatgggacg ggcgttggcg cgcgacccgc aactgtttct gtttgacgag cctttaagca
     8881 atctcgatgc caagctgcgg gtggaaatgc gcgccgaaat taagcgcctg caccaggtca
     8941 gcggcatcac cagcgtctat gtcacgcacg accagatcga agccatgacg ctgggcagcc
     9001 gcatcgcggt gatgaaagac ggcatcctgc aacaaattgg cacgccagac gacatttacc
     9061 gccgcccggc caatacctac gtggctggtt tcattggctc gcctactatg aactttataa
     9121 ctggcagcgc aagcgggacg ggcgctacag gccaattttc ctttgaaggc ggggccttgc
     9181 aattgccttg cccggcagtc ggcaaagtga cgctggggca gcgcccggag catattcaca
     9241 tcgaagcaca agccacctgg cgcggcgaag tgacgctggt ggagcccact ggtgccgaca
     9301 cctacgtggt ggtcaagacc ggctcagggc taatcacggt gcgaaccccg cccagcacca
     9361 ccgttaaagt gggtgaaaac attggtcttt cggtcagcag tcaacacaac aactggtttg
     9421 atgcgcagac tggcttgcgg ctggcttagg atgtggtcag aaataacttc ccctatttgc
     9481 accggcggat ttgggcg
//
LOCUS       DAOSLL010000108         9484 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6946_length_9484_cov_4.795949, whole genome shotgun sequence.
ACCESSION   DAOSLL010000108 DAOSLL010000000
VERSION     DAOSLL010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9484
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6946_length_9484_cov_4.795949"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(309..1904)
                     /locus_tag="PLB25_06510"
     CDS             complement(309..1904)
                     /locus_tag="PLB25_06510"
                     /inference="COORDINATES: protein motif:HMM:NF023277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HOE41265.1"
                     /translation="MKIVRKLNIAEPGQVGPRVQPWKVLVVDDEPDVRLLTSLNLRGF
                     TFAGRPLELIEACSSMQAKLRLTEHPDVALALIDVVMETDDAGLRLVEYIRKDLKNRL
                     IRLVIRTGQPGVAPERYVIDNYDIDDYKDKTELTVQRLYTTVRNSIKGFRDLQTIELN
                     RYGLSLILDVMPELYNLHRDKLEDYFQGVLMQLIGICNLGHSGMISSIDGLVATLEGE
                     DIRVQAGAGEFDASSGAMGRCQEIVKLCTQVVLNHVMPEGLRNGALVVPLLINTQILG
                     FVYLETGQDISEGDRELIQIMANQCAAALDNFRLHHSLEESYSEAIDMLGHVAEFKDS
                     ATGTHIRRIQAYTERLALAMGCTSAEALAYAKASRLHDVGKVGIPDHILRKPGPLTEE
                     EFAEIQRHTLIGDAVLKRSPSLAMARLVARSHHEKWNGLGYPDGCKGDAIPFVARLVA
                     VVDVFDALVSVRPYKGAWQKAAALAQIREGSGQHFDPVIADTFAALIEKGDMDDLIEA
                     AIQGTTCLAPDHSDHVIASTLGQ"
     gene            complement(1901..3454)
                     /locus_tag="PLB25_06515"
     CDS             complement(1901..3454)
                     /locus_tag="PLB25_06515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE41266.1"
                     /translation="MKFRTKTVLGVMLIQTVLLSVLVLSVLSQMRSSLRQQIELRADV
                     TAQLVAAAARDPMVSYDVSTLKSLATDLVASGQVAFVDFLDDRDRSMVTAGNGSGQPE
                     PLPDLSGENLLTRDVPILVAGKDYGHVRFGIRLDEMTALLELVKRKTVAMALGVMVAV
                     AVFSLFLGKLLTRRLNDLTVASHQIAAGNLSHQINEQGNDELADTARSFNQMSGRLQQ
                     RNRELLAAQHSLSALNATLEQRVAERTAALHEVNQRLQAEQERSLQAEKMASLGRMVA
                     GFAHEVNTPVGIAVGAVSQARELVKELGACLSQEEVAEEDLRSRLHTLEDVSELALNN
                     LRRTADLVHRFKRTAVDQTSEQMRPYELTEVIEDVMKSLRNEFKNTPIRFEVDCADCQ
                     PMYGPAGIVAQLLVNLMQNSRLHAFAEGTLGGCIRIQARTGVDTVSICYADDGAGMAA
                     ETLAHIFEPFYTTARGQGGSGLGLYIAHNLVTQALRGSIACHSALGQGTRFDITFPRQ
                     RLTSQTQQP"
     gene            complement(3480..4283)
                     /locus_tag="PLB25_06520"
     CDS             complement(3480..4283)
                     /locus_tag="PLB25_06520"
                     /inference="COORDINATES: protein motif:HMM:NF024376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOE41267.1"
                     /translation="MLGLCLVLGAVLGVHAEPLTVGITPLKPAAKLAEEWTPLLDEVA
                     KRAGLALRFRTATSIPVYGERLEKGEYDIAYMNPYDYQVYSASPGYRAFVRERGQALE
                     GVLLVRKDGRIKELRDINGARVSFPTPLAFAASLLTQAEMLKQGIKVDAKYVQSHDSV
                     LKSVAAGNFEAGGTILKVLQTADPALAADLRVLHKTAAYPSHPFAAHPRVPPEMVDKL
                     RQAFNSLRNDEVGRKLLAGVAFKGFESATDRDYDDVRHLDLKKLVEAVQ"
     gene            4445..4651
                     /locus_tag="PLB25_06525"
     CDS             4445..4651
                     /locus_tag="PLB25_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41268.1"
                     /translation="MQAFLWDKLFEAGLTEVDVQHQHLLKLINELGRRADIASLARPD
                     ETLTALAASWPCARHKRSQSPRHV"
     gene            complement(5242..6075)
                     /locus_tag="PLB25_06530"
     CDS             complement(5242..6075)
                     /locus_tag="PLB25_06530"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HOE41269.1"
                     /translation="MILRVWLQRSPLLGYLALTFAWSWACWALSPAIKPQLPTLATLL
                     MFAGSFGPSLAAVVVVASSRKCAGLRAWLVRCLQWRIGWGWWAFALLLPLAVMSLAAG
                     LHIALGGAIGTAPASGHLLMTVVNLPLVLLLGGPMGEEFGWRGFALPDLQDRLGWRAA
                     SVGLGLVWGVWHLPLFFIEGTAQADIPLALFLLSVVAMSVVLAWLVNNTAGSVVTALL
                     FHTAVNFWPSVVPVLPTEASYRAYALVVATLVMLALLALALTQQRGGADASPRCIEAK
                     P"
     gene            complement(6072..6953)
                     /locus_tag="PLB25_06535"
     CDS             complement(6072..6953)
                     /locus_tag="PLB25_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41270.1"
                     /translation="MTVLLWLGIGLAALVAVALGLRAVGAARWAELVGTHTRTLESGS
                     VGAQGRLPSPARFDAHELVALPPPVQRYFRAVLTDGQPIIAAATIDMAGTMNMSANAE
                     QWRPFTSNQRVVTHKPGFLWDAQVNLLPGVPALVEDSYMAGQGRLNAKLLGLFTVADS
                     HGEGEIARGEFMRYFAESPWYPTALLPSQGVRWEPVDDASASATIVDGRITLTLLFRF
                     DDAGLIASVHAESRGAGIGKDGAMVMLRWDCGLSNYQPQDGMLIPMTGEAAWVRPAGR
                     KVYFVGHVKKLRYEFLP"
     gene            complement(6983..8116)
                     /locus_tag="PLB25_06540"
     CDS             complement(6983..8116)
                     /locus_tag="PLB25_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat pathway signal protein"
                     /protein_id="HOE41271.1"
                     /translation="MITRRQFTASTPWLAAIPLALSGCSQGAAAEDYEAVAERIWQWA
                     PVTGDVTGAVTGLVGAALGRELVRCATLAPSSHNTQCWKFALDTNGRSITILPDLARR
                     CPAVDPDDHHVFVSLGCATENLIQAALAHGLKGEALFDVASDSVRVTLAPTPVQTTPL
                     FTAIPKRQCTRGDYDGKPLSSEELALLQRVGRSNTVRLLLLTERQAMEQTLDYVIQGN
                     TAQLADAAFVKELKAWIRFNGADAVRTRDGLYSVCSGNPNIPSWIGDLAFRWLLTPKG
                     ENDKVAQQVRSSAGIAVFVGQAADKSHWVEVGRCYQRFALQATALGIRNAFLNQPVEV
                     ASLRPPFAAALGLTGQRPDLVVRFGRGALLPRSLRRPVEAVLV"
     gene            complement(8113..8763)
                     /locus_tag="PLB25_06545"
     CDS             complement(8113..8763)
                     /locus_tag="PLB25_06545"
                     /inference="COORDINATES: protein motif:HMM:NF039641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6064 family protein"
                     /protein_id="HOE41272.1"
                     /translation="MVSAFTVEQFHGVFRDYNTAVWPAQWLLVAMGLAAVAAVLKPRP
                     WSGVAVSGILGGLWAWIALAYHLAFFARISPAAYGFAVLSAAGAAVFIWQGVIRQRLA
                     FRWVPGLKAMAGVVLIGFALVVYPVWSAYAGYPYPATPTFGLPCPTAIFTIGLLCFSA
                     TPTPRSPLVVPVLWCLIGAQAAFLLDVQPDLGLIAAGVVGIAMIASAGRPLRTQEP"
     gene            complement(9153..>9484)
                     /locus_tag="PLB25_06550"
     CDS             complement(9153..>9484)
                     /locus_tag="PLB25_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOE41273.1"
                     /translation="MSFSMPSLIGLIMLMGIATNNSILLVEYAIVARRGHDGSDGHPV
                     VAPMSRHDALIDACRKRTRPVIMTTLAMGVGTLPMAATGARRGGAGLSIKLAASAYRI
                     SAGSYCF"
BASE COUNT         1926 a         2992 c         2829 g         1737 t
ORIGIN      
        1 atgcgcagcc aatggcggcc tgaacacgaa ggggaaacta cgcactgtcc agttggtttt
       61 tggacgtttg caagacaacc aattttacca gtttagaagc aatttatcgt tttaaatcat
      121 agacttacgg cgttttttga acaccgaaac ttcagttata aaaagcgcgt aaccagctat
      181 ccaaaaagta gcacaaatca ccacccggcg tacgatgccc gcaaacccgg ttttttgagc
      241 cgctgcatga cgcgggatgc cggcggctcc gtggtggagc cgctgatgcc gcagtttggc
      301 aagtccgctt attgacccag cgttgatgcg atgacgtgat cggagtgatc cggggccaag
      361 caggtggtgc cttgaatggc cgcctcgatc aggtcgtcca tgtccccttt ttcgatcagg
      421 gctgcaaacg tgtcggcaat gaccgggtca aagtgctgcc cgctgccctc tcgaatttgc
      481 gccagcgcgg cggctttttg ccaggcacct ttgtagggcc gcacgctgac cagggcatcg
      541 aacacgtcta ctacggccac caatcgggca acaaagggaa tggcatcgcc cttgcagcca
      601 tcggggtagc ccaagccatt ccatttttca tggtggctgc gcgccaccag ccgagccatg
      661 gccagcgatg ggctgcgctt gagcacggca tcgccaatca gggtgtggcg ttgaatctcg
      721 gcaaactctt cctcagtcag tggcccgggc ttgcgcagaa tgtggtcggg tatgcccact
      781 ttacccacat cgtgcaagcg gctggccttg gcataggcga gggcctcggc tgaagtgcaa
      841 cccatggcca gtgccaggcg ctctgtatac gcctggatgc ggcgaatgtg cgtgcccgtg
      901 gcactgtcct tgaactcagc tacatgaccc aacatgtcga tggcttcgct atatgactct
      961 tccaggctgt gatgcagccg gaagttgtca agcgcggcgg cgcactggtt ggccatgatc
     1021 tggatcagct cgcggtcacc ctctgaaatg tcctgcccgg tttccaggta gacaaaaccc
     1081 agtatctggg tattgatcag caaaggcacc accagcgcac cgttacgcaa gccctcgggc
     1141 atgacatggt tcagcaccac ctgggtacag agcttgacaa tttcctggca tctgcccatg
     1201 gctccggacg aagcgtcaaa ctcacccgcc ccggcctgca ctcggatgtc ttcaccctcc
     1261 agcgtggcca ccagaccgtc gatggaagaa atcatccccg aatgccccag gttgcagatg
     1321 ccgatcagtt gcatcaacac cccctggaaa tagtcctcca gcttgtcccg gtggaggttg
     1381 tacagctcgg gcatcacgtc caggatcagc gacaggccat agcggttgag ctcgatggtc
     1441 tgcaggtccc tgaagccctt gatggaattg cgcaccgtgg tgtacaggcg ttgcacggtc
     1501 agttcggtct tgtccttgta gtcgtcgatg tcgtagttgt caatgacata gcgctcaggg
     1561 gccacgccgg gctgaccggt gcggatgacc agccggatca ggcggttttt gaggtctttg
     1621 cggatgtact ccactagccg cagaccggca tcatcggttt ccatgaccac atcgataagg
     1681 gccagcgcga cgtcagggtg ctcggtcagg cgtagcttgg cctgcatcga ggagcaagcc
     1741 tcgatcaatt ccagcggtcg gcctgcgaac gtaaaacccc gcaaattgag cgatgtcagc
     1801 agccgcacat cgggctcgtc gtcaacgacc agcaccttcc agggctgtac acgcggcccc
     1861 acttgtccag gttcagctat attgagtttt cggacaattt tcatggctgt tgtgtttgag
     1921 aagtaagccg ttggcgcgga aacgtgatgt cgaaacgcgt cccttggcca agcgcgctgt
     1981 ggcaagcaat gctgccccgc aaggcctgcg tgaccagatt gtgtgcaatg tacagcccca
     2041 agccgctgcc gccttgacca cgggccgtgg tgtaaaacgg ctcgaagatg tgtgccagtg
     2101 tttcggctgc catgcccgca ccgtcatccg cgtagcagat ggacaccgtg tctacccccg
     2161 tgcgggcctg gatgcggatg cacccgccca atgtgccctc cgcaaacgca tgcaggcggc
     2221 tgttttgcat caggttgacc agcaactgcg ccacgatgcc tgccggaccg tacatgggtt
     2281 ggcagtccgc acagtcgact tcgaagcgaa tgggggtgtt cttgaactcg ttgcgcaggc
     2341 ttttcatcac gtcttcaatg acctcggtca actcataggg gcgcatctgc tccgatgtct
     2401 ggtcaaccgc ggtgcgcttg aagcgatgca ccagatcggc cgtgcgcctg aggttgttca
     2461 gcgctagttc gctcacgtct tccagagtgt gaagcctgct gcgcaggtct tcttcggcca
     2521 cctcttcctg gctcaggcag gcacccagtt ccttgaccaa ctctcttgcc tgcgacaccg
     2581 cgccgacggc aatgccgaca ggtgtgttca cctcgtgtgc aaaacccgcc accatgcggc
     2641 ccagggaggc cattttttcg gcttgcagtg aacgctcctg ttctgcctgc agccgctgat
     2701 tgacctcatg caaggcagca gtgcgctcgg ccacccgttg ctccagcgtg gcgttcaggg
     2761 ctgacaggct gtgttgcgcg gccagcagtt cccggttgcg ctgctgtagc ctgcccgaca
     2821 tctggttgaa gctgcgtgcg gtgtcggcca gttcgtcgtt gccctgctcg ttgatctggt
     2881 gactgaggtt gccggctgca atctggtggc tggctacggt cagatcgttc aggcggcggg
     2941 tcagcagttt gcccaaaaac agcgaaaaca cggccacggc gaccatcaca cccagcgcca
     3001 tggccacggt cttgcgcttg accagttcaa gtagtgccgt catttcatcc agacgtatac
     3061 caaagcgaac atggccgtag tctttgcctg ccaccagaat gggcacgtca cgggtcagaa
     3121 ggttttcgcc ggacaggtcg ggcaaaggct cgggctgccc agacccattg ccagcggtca
     3181 ccatgctgcg atcgcggtca tccagaaaat caacaaacgc cacttgcccc gaggccacca
     3241 gatcggtcgc caggctcttc agggtagaca cgtcgtagct caccatcggg tcacgcgcgg
     3301 cggccgccac caactgcgcc gtgacatcgg ctctgagctc gatttgctgg cgcagcgaag
     3361 agcgcatttg ggacagcacg gacaacacca gcacagacag caacactgtt tgtatgagca
     3421 tgacacccag taccgtcttg gtgcgaaact tcatgaccta tggcgtgcgc gccccaggct
     3481 cattgcacag cctcgaccag tttttttagg tcaagatgac gcacatcgtc gtagtcacgg
     3541 tcagtcgcag actcaaaacc cttgaaagcc acgcccgcca aaagcttgcg cccaacttcg
     3601 tcgttgcgca ggctgttgaa agcctggcgc aatttgtcga ccatctccgg gggcacgcgc
     3661 gggtgtgcgg caaacgggtg cgaggggtat gcagcagttt tgtgcagcac ccgcaaatca
     3721 gcggccaatg cagggtcagc ggtttgcaac accttcagaa tggtgccacc tgcttcaaag
     3781 ttgcctgcag ccacactttt caacaccgag tcgtgcgact gcacgtactt ggcatccacc
     3841 ttgatgccct gcttcagcat ttcagcctgc gtcagcaggc tggccgcaaa cgccagcggg
     3901 gtcggaaaac tcacccttgc cccattgatg tcacgcagtt ccttgatacg accgtcttta
     3961 cgcaccagca acacgccctc cagcgcttgc ccacgctcac gcacaaaagc tcggtagccc
     4021 ggactggcgc tgtacacctg atagtcatag gggttcatgt aggcgatgtc gtattcacct
     4081 ttttccagcc gttcaccata taccgggatg ctggtggcgg tgcgaaaccg cagcgccaga
     4141 cccgcacgtt tagccacttc gtccagcaag ggggtccatt cttcggccag tttggcggct
     4201 ggcttgagtg gtgtgatgcc cactgtcagc ggctccgcat gtacacccag caccgcgccc
     4261 aagaccaaac ataggcccaa caacctgttt cgtatcatgg taacaactcc gtgactgaaa
     4321 acaggtttga tgttacccct caacacatgt gttcacgggc gcgaacgcat gaagttttca
     4381 tcatcaaata gggcctcgta gcacgctcca ctggcccgtt tgcacacctc acctggagca
     4441 cgcaatgcaa gccttcttgt gggacaagct gtttgaagcc ggcctgaccg aggtagatgt
     4501 ccagcaccag cacctgttga aactgatcaa tgaattgggt cggcgggcag acattgccag
     4561 cctggcccgc ccagacgaaa cgctgacggc actggccgca agttggcctt gtgctcgcca
     4621 caaacgcagc caatcccccc ggcacgtctg acttaccgcc ctgttcagtc accaatttac
     4681 acgctgaccg acttcgccgt ctggtgcggg ttgcgctgcc agactccaat cttgctcgat
     4741 tttcgccccg tcatccatct gtgcatcagc accgacatcc ctctgctgtg aaatgcgtgc
     4801 cggggacgag ccattgccag cgtccggtct ggaagaggca aatcacaaac tgctactacc
     4861 ggtaagggtc gttatgcaca tctctgcata agtgcccttg tgcacagctc gcacttatga
     4921 acagtttgcg tgggtcagac tggcggcgcc ctatgcagag ggcaacacac ggggacattg
     4981 ggctctacaa aattacagat ttttcaagaa ctccaacagt gattcggtcg cttgaggcgc
     5041 tgcgcaggaa tttctggatc ctgtagacca gccagggcct attctttcat tgccaaatcg
     5101 gcttcgatgc attggtgggt ggtagacagg gggcgctggc ggcgaaccca gtgcaaaagc
     5161 caaccgcact gcgccgcggc ccaaacgcca tctatcggat cagagccaaa ggcacaaaga
     5221 cctgcgcgag agtgaggtgg ctcacggctt cgcctcgatg catcgaggcg acgcgtcagc
     5281 cccaccgcgc tgctgcgtca gcgccaacgc cagaagagcc agcataacca gcgtggcgac
     5341 caccaacgca taggcccggt agctcgcttc ggtgggcaag actggcacca cagacggcca
     5401 gaagttgacc gcggtgtgaa acagcagtgc cgtcaccacg ctgcccgcgg tgttgttgac
     5461 cagccaagcg agcacgaccg acatggccac gacgctcagc aggaacaagg cgagcgggat
     5521 gtcggcttgc gcggtgcctt cgatgaaaaa cagcggcagg tgccacaccc cccacaccag
     5581 ccccagaccc acgctggccg cgcgccatcc cagccggtct tgcagatcag gcagagcaaa
     5641 tccgcgccag ccgaattctt cccccatcgg cccacccagc agcagaacca gcggtaggtt
     5701 gacgacggtc atcagcagat gtcccgatgc tggcgcggtg ccgatggcac cacccagtgc
     5761 gatgtgcagg cccgccgcca aggacatcac ggccagcggg agtagcagtg cgaaggccca
     5821 ccaaccccag ccgatgcgcc actgcaggca gcgcaccaac caggcgcgca gacccgcgca
     5881 ctttcggctg ctggcgacca caacgacggc ggccaggctg ggcccgaagc tgccggcaaa
     5941 catcagcagc gtcgcaagcg tcggcaactg aggcttgatc gcgggcgaca gcgcccagca
     6001 agcccaggac caggcaaagg tcagcgcaag gtagccgagc agtggcgagc gctgcagcca
     6061 cacccgcaag atcatggcag gaactcgtag cgcagcttct tgacgtgccc gacgaaatac
     6121 accttgcggc ccgcaggccg cacccaggcg gcttcaccgg tcatcgggat cagcatgcca
     6181 tcctgcggct ggtagttcga taagccgcag tcccagcgca gcatgaccat ggccccgtcc
     6241 ttgccgatgc cagcgccacg cgactcggcg tgcaccgatg cgatcaggcc ggcatcatcg
     6301 aaccggaaca gcaaggtcaa ggtgatccgg ccatcgacaa tggtggcgct ggctgaagcg
     6361 tcgtccacag gctcccaccg caccccctgg ctgggcagca gtgcggtggg ataccacggg
     6421 ctttcggcaa agtagcgcat gaactcgccg cgagcaatct cgccttcgcc gtgtgagtcg
     6481 gccaccgtga acaacccgag caacttcgca ttcaacctgc cctggccggc catgtagctg
     6541 tcttccacga gcgcgggaac gccgggcaac aggttgacct gggcgtccca caggaaaccg
     6601 ggcttgtggg tgacaacccg ttgattcgac gtgaacggcc gccattgctc ggcgttggcc
     6661 gacatgttca tggtgcctgc catgtcaatg gtggccgcgg caatgatcgg ctgtccgtcc
     6721 gtcagcaccg cgcggaagta gcgttgtacc ggcgggggaa gcgcgacgag ctcatgcgca
     6781 tcaaagcgcg cgggtgacgg cagtcggcct tgcgcgccaa cgctgccgga ttcaagcgtg
     6841 cgggtgtggg taccaaccag ctcggcccac cgcgcggcgc cgactgcgcg taatcctagg
     6901 gcaacggcga ccagcgcggc aagcccgatt cccagccaca gcagcacagt cattgcagtt
     6961 cctttggttc gcggtaggtt ggtcacacca gaacggcttc gaccggccgg cgcagcgagc
     7021 gcggcaacaa tgcgccgcga ccgaatcgca cgacgaggtc cggccgctgg cccgtcaagc
     7081 ccagtgcggc tgcgaaaggc gggcgcagtg atgccacttc gacgggctgg ttcaaaaagg
     7141 cgttgcggat gcccagcgcg gtagcctgca gggcaaagcg ctggtagcag cgccccacct
     7201 ccacccaatg cgacttgtcg gcggcctggc cgacgaacac cgcgatgccg gcggaactgc
     7261 gcacctgctg cgcgaccttg tcgttctcgc ccttcggggt gaggagccag cgaaatgcca
     7321 gatcgccgat ccacgacggg atgttcgggt tgcccgagca gacgctgtac agcccgtcgc
     7381 gcgtgcgaac ggcatcggcg ccgttgaatc gtatccaggc cttcagctcc ttgacgaagg
     7441 cggcatcggc cagttgcgca gtattgcctt gaatcacgta gtccagcgtt tgctccatgg
     7501 cctggcgctc agtcagcagc agcaggcgca cggtgttcga cctgccgacg cgctgcagca
     7561 acgccagttc ctcgctcgac aagggcttgc cgtcgtagtc accgcgtgtg cattggcgct
     7621 tggggatggc cgtgaacaag ggcgtggtct gaaccggcgt tggcgcaagc gtcacccgca
     7681 ccgaatcgct ggccacgtcg aacagcgctt cgcccttgag gccgtgggcg agcgcggcct
     7741 ggatcaggtt ctcggtggcg cagcccagcg agacaaagac gtgatggtcg tccgggtcca
     7801 ccgccgggca gcgacgcgcc aggtcgggca ggatggtgat cgaccggcca ttggtatcca
     7861 gtgcgaactt ccagcactgc gtgttgtggc tggacggggc cagcgtggca cagcgaacca
     7921 gctcccgacc gagcgcagcg ccgaccagac ccgtcacagc acccgtcacg tcacccgtca
     7981 ccggagccca ttgccagatg cgctcggcga ccgcttcgta gtcctctgcc gcggcccctt
     8041 gggagcagcc ggacagcgcc agcgggatgg ccgccagcca aggcgttgaa gcggtgaact
     8101 gcctgcgtgt gatcatggtt cttgagtcct aagtggtcgg cccgcgctcg cgatcatagc
     8161 gatgcccacc acgcctgcag cgatcagtcc gaggtcaggc tggacatcaa ggagaaaagc
     8221 cgcctgggct ccgataaggc accatagcac cggaacgacc aacggactgc gcggcgttgg
     8281 cgttgctgag aagcacagca ggccgatggt gaagatggcc gtcgggcacg gcaggccgaa
     8341 ggtcggggtc gccggatacg gataaccggc gtaggccgac cagaccgggt acaccaccaa
     8401 ggcgaagccg atgagcacga caccagccat tgccttcagg cccggcaccc atcgaaaggc
     8461 gagcctttgc cgaatcacgc cctgccagat gaacacggct gcgccagccg cagataggac
     8521 cgcgaagcca taggccgcag gactgatgcg cgcgaagaag gccaggtgat aggccaaggc
     8581 gatccaagcc caaagaccgc cgaggatgcc ggacaccgca acgccggacc agggccgcgg
     8641 cttgagtacc gctgccactg ccgccaagcc cattgcgacg aggagccact gggccggcca
     8701 cacggcagtg ttgtagtcac ggaaaacacc gtggaactgc tcgaccgtga acgcagacac
     8761 cataggcgtt cagattaggc gcaaaggcgg cggcagtctg tgtgctgccg aacacggcgc
     8821 gacggcgata ggttgagcca ttcgaacacc ttgccatagc aaccggcgcg cggaccgcct
     8881 gcgcgagtca ggttttaaac gccacaggtc accagcgaga gcaaggcgat tgcgcagaag
     8941 agcgctgctg agacagtggc gattgaggtt cgttgggctg catgaaagcg ggcgatggtg
     9001 ggtggcggct gctggccaac tgcgggtgca gcgaccatcg aaatcaatgc catattgcca
     9061 ccctttcaat gcctatttgt acgaccggtt tgccttgcgt ttgcaatgcc tgcgccaggg
     9121 cgcctggata caaatgattc agcaatggtg attcaaaaac aatagctgcc agcgcttatc
     9181 ctgtatgcgc tagcagccaa tttgatgctt aaccctgcgc caccccgccg cgccccagtc
     9241 gccgccatcg gcaacgtgcc tacacccatg gccagcgtag tcatgatgac cgggcgggtg
     9301 cgtttgcggc aggcatcgat caacgcgtcg tgacggctca tcggtgccac aaccggatgg
     9361 ccgtcgctgc cgtcgtgccc tcggcgcgcc acgatggcgt actccaccag caaaatcgaa
     9421 ttattcgtgg caatgcccat cagcatgatc aggccgatca gcgacggcat ggaaaaactc
     9481 atgc
//
LOCUS       DAOSLL010000109         9452 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6965_length_9452_cov_6.584229, whole genome shotgun sequence.
ACCESSION   DAOSLL010000109 DAOSLL010000000
VERSION     DAOSLL010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9452)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9452)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9452
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6965_length_9452_cov_6.584229"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="PLB25_06555"
     CDS             <1..1186
                     /locus_tag="PLB25_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41274.1"
                     /translation="RVGRFLATDDTSGAIKLRYYMGGESVANYSEFFIWAVQKQPGHT
                     DPMHGVPLQLMADPGSGLAGAFVNLVRRLRIKLIINAPGNPRAKGQVERSQNIVEMGF
                     EHQFRSHRPKNLADLNARAQVWANHYNATQIHSRHGLTRYAKWREIMPEQLRTAPTIE
                     VCRELLTAPHKDCRVDNYGYVQFGGGGRKWDVRSVPGGVAAGQKIAITYSAYNDQEVF
                     AVLVGADGHELLHPCPLLQYDDHGFHADARVKGAGYQALKDTAADTNRKQANLLATGA
                     DTLEGAAIARRAKGFEVFNGAVRFDHLAAELAALPVSLARAAQPLQLHTSTPAQPVAA
                     RLLTHFEAAAALTGMGVSASAERNAAILAYANGVPEDDLPAIKARLTVRAGLRVVNGG
                     SL"
     gene            1261..2496
                     /locus_tag="PLB25_06560"
     CDS             1261..2496
                     /locus_tag="PLB25_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE41275.1"
                     /translation="MIPENASQAPTASPAAELLKTLGIPQADLCRATGMSRATISRLV
                     VHGQWPVYQADLVLEDIEGYLVKRGLTPTQVQRITLALQNKLAPEVSHTPEAVPQVPT
                     ETETEKEATMLLQNHELYIETQDHFKLERNPFVDDINSRADVFTSAGTRRVRNAMLNA
                     ATQHGFIAVVGESGSGKSTLRKDLEERIRIEKLPIRVIKPYIVQMEPTETRGKPMKSA
                     EIADAIARTLAPGMALKARADSRFEQVHELLCASASAGYSNVIVIEEAHRLPLTTLRH
                     LKGFMELEDGIRRLLGVCLIGQPELSRLLAAENRDIREIVQRCTRVELGPMDADLEAY
                     VQHKFERAGADVRSVLAADAYGAIRAKLIQVARGGNRMNDTRNLCYPLVVNNLLCKAM
                     NFAAQEHWPLVDKQVIAGC"
     gene            2499..2645
                     /locus_tag="PLB25_06565"
     CDS             2499..2645
                     /locus_tag="PLB25_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41276.1"
                     /translation="MKTYRITITQANGQQALFLGIYSDGFRAVIAAIDNFPTAQRISA
                     RRLP"
     gene            2642..2947
                     /locus_tag="PLB25_06570"
     CDS             2642..2947
                     /locus_tag="PLB25_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41277.1"
                     /translation="MMIKKHRTCDQLGVCQGLGADHCPDCDAWECDVRCAPHPGEAAQ
                     PTYPFAPGIIEGPPVPGGPFDAPWGISLKDAAFMLGAVLLCSAAGGLLVGWLSGWPK"
     gene            2944..3561
                     /locus_tag="PLB25_06575"
     CDS             2944..3561
                     /locus_tag="PLB25_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41278.1"
                     /translation="MSGELSCPVCGTTISMEHLLINADDRSVVLRLLAMGTPIGAAVG
                     QYTRLFDPPKSRLTMRKQARIVKELVPDIERRAITHRGRDWAVSLPMWEAGIDQMLDA
                     RNAGKLDLPMTSHRYLYAILAALADKAEGEQEAEALAAARAAPRQATVTVRGQPLPMG
                     QALQQVFGNQDPTLAKLDAERGTYAPMPAAARAQLAKLKNPGAKP"
     gene            3558..4019
                     /locus_tag="PLB25_06580"
     CDS             3558..4019
                     /locus_tag="PLB25_06580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41279.1"
                     /translation="MIEKTYHTQNPVNIRILNYVATMGQCTAAHLHAMFLPDEASRDE
                     RKSMVNKLSYLVCTEQLLRHGKGETAVLRIGPHAGAPGVRGRREKHMANARAAALAPC
                     VSAGPGAYRPQVASAPDHNHMAVDVYVPPPGPAMRRGALQHESCASRGCRC"
     gene            4065..4589
                     /locus_tag="PLB25_06585"
     CDS             4065..4589
                     /locus_tag="PLB25_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HOE41280.1"
                     /translation="MATRIKSKTLAEVPQSKNDCAESIRTIGDLQREFERNRAAMNDA
                     IAAITQEHQPLLAALSERIQALQTGVQAWCEAHRVDLCGENDKLGKTANLVTGEVSWR
                     IRPPSVTVRDVDGVLDTLLRMGLGRFVRVKNEPNKEAMLNEPDAVRGIAGITIKSGVE
                     DFIVTPFEAQAEVA"
     gene            4586..4855
                     /locus_tag="PLB25_06590"
     CDS             4586..4855
                     /locus_tag="PLB25_06590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41281.1"
                     /translation="MNQQQAYDCLPGFVAGKHRRSCRNCVYGRHITPATLAHINDAPM
                     QCGYKFCTVSAMGICYAFRPGEAIDCTSQAHLGDQSNADATGVVS"
     gene            4864..5325
                     /locus_tag="PLB25_06595"
     CDS             4864..5325
                     /locus_tag="PLB25_06595"
                     /inference="COORDINATES: protein motif:HMM:NF018011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HOE41282.1"
                     /translation="MPRQSHLAAIHVAQKALGLSADDASALKLAITGKASSADMSEVQ
                     RKKYLAHLSGLQERAGLIAPRPQKRTYVQRNADDDQDERWGKARALWHALAAAKVVHT
                     NTDAALMSYIKRQTKLEHWRFLNSYQVNSVIEALKKWCTRSGIDPNPAPRS"
     gene            5330..5719
                     /locus_tag="PLB25_06600"
     CDS             5330..5719
                     /locus_tag="PLB25_06600"
                     /inference="COORDINATES: protein motif:HMM:NF020345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HOE41283.1"
                     /translation="MALPDASDGRMAERRHELYADLMDLLQRLLAEYDVGSNEALLIA
                     SDMADRLANHWGGQNITFPKEYRRKLSRLELEIFDAFKGHNLSAIAQTYNISERGLRK
                     LIARVTKRLRCGNQPGLFEPPALPDQH"
     gene            5798..6730
                     /locus_tag="PLB25_06605"
     CDS             5798..6730
                     /locus_tag="PLB25_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015985059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HOE41284.1"
                     /translation="MPQTPPLQIFKPGLHTAMNGAVLQFSEADLQKTVAAYDPTKHEA
                     PLVVGHPAHDDPAYGWVQALQFAGGALDAIPAQVNPDFAEMVASGAFKKISASFYAPN
                     SPANPVPGIYYLRHVGFLGATAPAVKGMRAPQFADTEEGVLTFEFAEPPPITTHQEPT
                     VIESEKDALEAENTRLRAELAAARRTAVHAANVAFCESCAGVLPDHRALAVEIADQLA
                     AQPVPLEFGEGDQKAPLLTHFKAFLKALAAPVQFGEAATRERAAPDQPAASDADAQFA
                     DTATPERLAQHRAVLAYMAAHKTDYQTAANAVVK"
     gene            6777..7697
                     /locus_tag="PLB25_06610"
     CDS             6777..7697
                     /locus_tag="PLB25_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015985049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41285.1"
                     /translation="MGRLSKLRVVDPVLTNLATGYSNEQLVGDQLMPFVTVEKEGGKI
                     PLFGKDHFKVYQTERALRAKSNRINPDDIGELDVTLDEHDLEYPIDYREDAESAFPLQ
                     ARATNAVVEGIRLRHEKMVADMVQNPANYAAGNKLALSGASCFTDAASDPEGVVMDAK
                     SAVRAKVIKEPNTMVIGYAAWLTMKKHAKLKAILSDNRPRLVQLADLRDIFEIPNIVV
                     GRAVYASDAGVTADIWGDNIVLAYVPGAMGGSRSPYEPSFGYTLRKRGNPVVDTRTED
                     GKIELIRNTDIFRPFLLGADAGYLISNVNL"
     gene            7746..7955
                     /locus_tag="PLB25_06615"
     CDS             7746..7955
                     /locus_tag="PLB25_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41286.1"
                     /translation="MKTAPDAQQRTDTPAWQVGAVPVCHDGVRYAPGAVIVLTSAQAA
                     RLGLQQVVKDSLTSGAATSPSLTPN"
     gene            7973..8299
                     /locus_tag="PLB25_06620"
     CDS             7973..8299
                     /locus_tag="PLB25_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HOE41287.1"
                     /translation="MKTEKILMVTTILAAAALARFRLVDYSGNPTSAAERALGVAVTN
                     FDTGEQAGVAVKGEVLVEAGGAIAVGAEVESDASSRVVTKNAGVVVGVARDAAAAAGD
                     IIRVLI"
     gene            8308..8811
                     /locus_tag="PLB25_06625"
     CDS             8308..8811
                     /locus_tag="PLB25_06625"
                     /inference="COORDINATES: protein motif:HMM:NF018704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1320 family protein"
                     /protein_id="HOE41288.1"
                     /translation="MSHYSTIDDLAQAATNGWDELAQRAGGALVDGALLQATALLEDR
                     SAWSPMARAQADAALLRITALLEAASRHADTYLFPRYRAVMPLTPELVQASSLPQAVA
                     AIALKRLYGASLPDDVRNGTKWADDYLRDLNKGVVSLGAVDDTVATPAGRMVSRVPDK
                     AFDWDGY"
     gene            8814..9257
                     /locus_tag="PLB25_06630"
     CDS             8814..9257
                     /locus_tag="PLB25_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41289.1"
                     /translation="MDLDLLALETALLERLRTALAGQMPPVHVLSQDDVAAAAEEKQL
                     VPAVHVIYQNYRVLESRSDGKAARIEQIWLAVVATRNTRNLASATSSRRQAGLLGGQV
                     AKALMGWRAPGTATPWRLVDGPGAAFGTGFGYLPIAVAAEMTVTA"
BASE COUNT         1975 a         3032 c         2899 g         1546 t
ORIGIN      
        1 gcgcgtgggc cgctttctgg ccaccgacga caccagcggt gccatcaagc tccgctacta
       61 catgggcggt gagtcggtgg ccaactacag cgagtttttt atctgggccg tccaaaagca
      121 acccggccac accgacccca tgcacggcgt gccgttgcag ctcatggccg accccggctc
      181 tggcctggcc ggtgcctttg tcaacctggt gcgccgcctg cgcatcaagc tgatcatcaa
      241 cgcccctggc aacccgcgtg ccaaggggca agtagagcga agccagaaca ttgtggaaat
      301 gggctttgaa caccagtttc gcagccaccg cccaaagaac ctggctgacc tcaatgcacg
      361 cgcccaagtg tgggccaacc actacaacgc cacccaaatt catagccgcc acggcttaac
      421 ccgatacgcc aaatggcgcg aaatcatgcc tgagcaactg cgtaccgccc ccaccattga
      481 agtctgccgc gagctgctca ccgcccccca caaagactgc cgggtggaca actacggata
      541 cgtgcagttt ggcggtggtg ggcgcaagtg ggacgtgcgc agcgtacccg gcggtgtggc
      601 ggcaggccaa aagattgcca tcacctacag cgcctacaac gaccaagagg tatttgccgt
      661 gctggtgggg gccgacggcc acgaactgct gcacccctgc ccgctgctgc aatatgacga
      721 tcacggcttt catgccgatg cccgtgtcaa aggagcaggc taccaggcgc tcaaagacac
      781 cgctgcagac accaaccgca agcaggccaa cctgctggcc accggtgctg acaccttgga
      841 gggtgcagcc attgcccgca gggccaaagg ttttgaggtg tttaacggtg cagtgcgctt
      901 tgaccacctg gccgcagagc tggcagcact gccggtaagc ttggcgcgtg ctgcccagcc
      961 actgcaactg cacaccagca cccccgcgca accagtggct gccagattgc tcacccattt
     1021 tgaggctgct gcggcgctta ctggcatggg cgtgagcgcc tcggctgagc gcaatgcggc
     1081 cattctggcc tatgccaatg gggtgcctga agatgatttg ccagccatca aggcgcgcct
     1141 gacggtgcgt gctggcttgc gggtggtcaa cgggggttcg ctatgagccg caaaccgggc
     1201 gcaaatgcgt caaaccgtgt ttctaaatgt tccaaagaaa aaaaccatac cagtgccgcc
     1261 atgatccctg aaaacgcctc acaagcgccc acagcgagcc cagccgctga actgttgaaa
     1321 acccttggga ttccacaagc cgacctgtgc cgcgccaccg gtatgagccg cgccaccatc
     1381 agccgcctgg tggtgcatgg ccagtggccg gtctatcagg ctgatttggt gttggaagac
     1441 attgagggct acctggtcaa gcgtggcctg acccctaccc aagtgcagcg catcaccctg
     1501 gcgttgcaaa acaagttggc cccagaggta tctcacaccc ctgaggccgt tccccaagtc
     1561 ccaactgaaa ctgaaactga aaaggaagcc accatgctac tgcaaaacca tgagctgtat
     1621 atcgaaacgc aggaccactt caagttagag cgaaacccgt ttgttgacga catcaacagc
     1681 cgcgccgacg tgttcaccag cgccggcacg cgccgggtgc gcaatgccat gctcaacgcc
     1741 gccacacagc acggctttat tgccgtggtg ggtgaaagcg gcagcggcaa aagcacgctg
     1801 cgcaaagacc tggaagagcg catccgcata gagaaactgc ccattcgcgt gatcaagcca
     1861 tacatcgtgc aaatggagcc cactgaaacc aggggcaagc cgatgaagag cgccgaaatt
     1921 gccgatgcca ttgcgcgcac gctggccccc ggtatggcgc tcaaagcccg ggccgactcg
     1981 cgctttgagc aggtgcacga gctgctgtgt gccagtgcct cagcaggcta cagcaatgtc
     2041 attgtgatcg aggaggcaca ccgcctgccg ctgaccacgc tgcgccatct caaaggcttc
     2101 atggagttgg aggacggcat acgccgcctg ctgggcgtgt gcttgatcgg ccagcccgag
     2161 ctatcgcgcc tgctggccgc agaaaaccgc gacatccgcg agatcgtgca gcgctgcacc
     2221 cgcgtcgaac tcggccccat ggacgccgac ctggaggcct acgtgcaaca caagtttgag
     2281 cgcgccggcg ctgatgtgcg cagcgtgttg gccgccgatg cctacggtgc cattcgcgcc
     2341 aagctgatac aggtggcccg tggtggcaac cgcatgaacg acacgcgcaa cctgtgttac
     2401 cccctggtgg tgaacaacct gctgtgcaag gccatgaact ttgccgccca agagcactgg
     2461 ccgcttgtag ataagcaagt gattgcgggg tgctagccat gaaaacctat cgcatcacca
     2521 tcacccaggc caacggccag caagccctct ttttgggcat ctacagcgac ggctttcgcg
     2581 ccgtgattgc cgccatcgac aacttcccca ccgcacaaag aatttcagcc cggagactgc
     2641 catgatgatc aaaaaacacc gtacctgtga ccagcttggc gtctgccagg gcctgggcgc
     2701 tgaccattgc cctgactgcg atgcatggga gtgcgacgtg cgctgcgccc cgcacccggg
     2761 cgaagctgcc cagccaactt acccgtttgc ccccggcatc atcgaaggcc cgccggtacc
     2821 cggtggcccg tttgatgcgc cctggggcat cagtctgaaa gatgccgcct tcatgctggg
     2881 ggcggtgctg ctgtgcagtg ctgccggtgg cctgctggtg ggctggctca gtgggtggcc
     2941 aaaatgagcg gcgaactgag ctgcccagtg tgcggcacca ccatcagcat ggagcacctg
     3001 ctgatcaacg cggacgaccg ctctgtggtg ctgcgcctgc tggccatggg cacgcccata
     3061 ggtgcggcgg tgggccaata cacccgcctg tttgacccgc ccaaaagccg cctgaccatg
     3121 cgcaagcagg cccgcattgt caaggagctg gtgcccgaca tagagcgccg cgccatcacc
     3181 caccgcggcc gcgactgggc cgtgtcgctg cccatgtggg aggccggcat tgaccagatg
     3241 ctggacgccc gcaacgctgg caagctggac ctgccaatga ccagccaccg ctacctgtac
     3301 gccatcttgg ccgcgctggc cgacaaggcc gaaggcgaac aagaggccga agccctggcc
     3361 gctgcccgcg ccgccccacg ccaggccacg gtgacagtgc gcggccagcc cctacccatg
     3421 gggcaggcgc tgcagcaagt gtttggcaac caagacccaa cgctggccaa gctggatgcc
     3481 gaacgcggca cctatgcacc catgccagcc gcagcacggg cgcaactggc caagctcaaa
     3541 aaccccggag ccaaaccatg attgagaaaa cctaccacac ccaaaacccg gtcaacatcc
     3601 gaatactcaa ctacgtggcc accatgggcc agtgcaccgc agcgcatctg catgcgatgt
     3661 ttttaccaga tgaagccagc cgcgatgagc gcaagagcat ggtcaacaag ctgagctacc
     3721 tggtgtgcac cgagcagttg ctgcgccatg gcaagggcga aacggccgtg ctgcgcattg
     3781 gcccgcatgc cggtgcgcca ggtgtaaggg gcaggcgtga aaagcacatg gcaaatgcac
     3841 gagctgcagc gctggccccc tgtgtgagcg ctggccccgg tgcctaccgg ccgcaggtgg
     3901 ccagtgcccc ggaccacaac cacatggcgg tcgatgtgta cgtgccgcca cccggcccgg
     3961 cgatgcgccg tggcgcacta caacacgaaa gctgtgccag ccgtggttgc aggtgctaaa
     4021 gccgttacca atttatttcc caccaagaaa aggtctttcc aaaaatggct acccgaatca
     4081 aatccaaaac cctggccgaa gtgccgcaaa gcaagaacga ctgtgccgaa agcattcgca
     4141 ccattggcga cctgcagcgc gagtttgagc gcaaccgcgc tgccatgaac gatgccattg
     4201 ccgccatcac ccaggagcac cagcccctgc tggcggcgtt aagtgagcga atccaggcgc
     4261 tgcaaaccgg cgttcaagcc tggtgcgagg cgcaccgcgt tgacctgtgc ggcgagaacg
     4321 acaagctggg caaaacggcc aacctggtca ccggcgaggt gagctggcgc attcgcccgc
     4381 cgagtgtgac ggtgcgcgat gtcgatggcg tgctcgacac cctgctgcgc atgggcctgg
     4441 gccgctttgt gcgcgtgaaa aacgagccca acaaggaggc catgctcaat gagcccgacg
     4501 cggtgcgcgg cattgctggc atcaccatca aaagcggggt ggaagacttc atcgtcacgc
     4561 cgtttgaggc acaggctgag gtggcatgaa ccagcagcag gcctatgact gtttgcctgg
     4621 cttcgtagcc ggcaaacatc gcagaagctg ccgcaattgc gtctacggcc ggcacatcac
     4681 gcccgctacg ttggcccata tcaacgatgc gccgatgcag tgcggctaca agttttgcac
     4741 ggtcagcgca atgggcatct gctatgcctt tcggccgggc gaggcaattg actgcaccag
     4801 tcaggcccac ctgggtgatc aaagcaacgc cgatgccacg ggcgtagtgt catgagccaa
     4861 accgtgcccc gccaatccca ccttgccgcc atccacgtgg cgcaaaaggc ccttggtctg
     4921 tcggctgacg atgccagcgc gctaaagctg gccatcaccg gcaaggccag cagcgctgac
     4981 atgagcgagg tacagcgcaa aaagtacctg gcgcacctca gcggcctgca agagcgcgcc
     5041 gggctgattg cgccgcgccc gcaaaagcgc acctatgtgc agcgcaacgc cgacgacgac
     5101 caggacgaac gctggggcaa ggcgcgcgcc ctgtggcacg ccctggccgc tgccaaggtg
     5161 gtgcacacca acaccgatgc cgccctgatg tcctacatca agcgccagac caagctggag
     5221 cactggcgct ttttgaacag ctaccaggtc aacagcgtga tagaagcgct gaaaaaatgg
     5281 tgcacccgct ctggcattga ccccaacccc gcaccaagga gctaacccca tggcattgcc
     5341 agatgcatct gacggccgca tggccgagcg ccggcacgag ctgtacgctg acctgatgga
     5401 cctgctgcag cgcctgctgg ccgagtacga cgtgggcagc aatgaggcgc tgctgatagc
     5461 cagcgacatg gccgaccgcc tggccaacca ctggggcggg caaaatatca ccttccccaa
     5521 ggaataccgg cgcaagctaa gtcggcttga gctggagatt tttgacgcct tcaaggggca
     5581 caatctgtct gccatagcgc aaacctacaa catcagtgag cgcggcctgc gcaagctgat
     5641 tgcccgggtg accaagcggc tgcgctgtgg caaccagccc ggcctgtttg agcccccggc
     5701 gctgccagac cagcactgat cagaccgccc accaggtgca gcagaatctg ccctggttcc
     5761 aaatacattg gcacccggcc ccggcacagt accgggtatg ccacaaaccc ccccgctaca
     5821 aatcttcaag cccggcctgc acaccgccat gaacggtgct gtgctgcagt tttctgaagc
     5881 agacctacag aaaactgtag ccgcttacga ccccaccaag cacgaggccc cgctggtggt
     5941 aggccacccg gcacacgacg accccgccta tggctgggtg caagcgctgc agtttgcggg
     6001 cggtgccctg gatgccatac ccgcgcaagt caaccctgac tttgccgaga tggtggccag
     6061 cggcgcgttc aaaaaaatca gcgccagttt ctatgccccc aacagcccgg ccaacccggt
     6121 gccaggtatt tattacctgc gccatgtggg ctttttgggt gccacagcac cagccgtgaa
     6181 aggcatgcgt gcaccacagt ttgccgacac cgaagagggc gtgctgacct ttgagtttgc
     6241 cgagccaccc cccatcacca cccaccagga gcccaccgtg atcgagtcag agaaagatgc
     6301 cctagaggct gaaaacaccc gcctgcgtgc cgagctggcc gccgcgcgcc gtaccgccgt
     6361 gcatgccgcc aatgtggcct tttgcgaaag ctgtgccggt gtgctgccag accaccgggc
     6421 gctggcggta gagattgccg accaactggc ggcccaaccg gtgccgctgg agtttggcga
     6481 gggtgaccaa aaggccccgt tgctgacgca cttcaaggcc tttttgaaag cattggcagc
     6541 accggtgcag tttggcgaag ccgccacccg cgagcgtgct gcaccagacc aacctgccgc
     6601 cagcgacgca gacgcccagt ttgccgacac cgccaccccc gagcgcctgg cacagcaccg
     6661 cgctgtgctg gcctacatgg cggcgcacaa aaccgactac cagacggctg caaacgccgt
     6721 ggtgaagtag tccggcgctg ctgcattgac cacgaaattt aaaggagctt tttgatatgg
     6781 gacgtttaag caaactgcgg gtggttgacc cggtgttgac caacctggcc actggctaca
     6841 gcaacgagca actggtgggc gaccagctta tgccgtttgt gacggtggag aaagaaggcg
     6901 gaaagattcc actgtttggc aaagaccact tcaaggtgta ccagaccgag cgggcgcttc
     6961 gggctaagag caaccgcatc aaccccgacg acattggcga actggacgtg acgctcgacg
     7021 agcacgacct ggaatacccg attgattatc gggaggatgc cgagagcgcc ttccccctgc
     7081 aggcacgcgc caccaatgcg gtggtggagg gcattcgcct gcggcatgaa aagatggtgg
     7141 ccgacatggt gcaaaacccg gccaactatg ccgctggcaa caagctggcg ctgagcggtg
     7201 caagctgctt taccgatgca gccagcgacc ccgaaggcgt ggtgatggac gccaagtcag
     7261 cagtacgggc caaggtgatc aaggagccca acacgatggt gattggctac gctgcctggt
     7321 tgaccatgaa aaagcatgcc aagctcaaag ccatcttgag cgacaaccgc ccccgcctgg
     7381 tgcagttggc tgacctgcgc gacatctttg agatacccaa cattgtggtg ggccgcgccg
     7441 tgtatgccag tgacgctggc gtgacggccg acatctgggg cgacaacatc gtgctggcct
     7501 atgtgcccgg tgccatgggt gggtcgcgca gcccgtatga gccgagcttt ggttacacct
     7561 tgcgcaagcg gggtaacccg gtggtggaca cccgcacaga agacggcaag attgagctga
     7621 ttcgcaacac cgacatcttc cggccatttt tgctgggcgc agatgcgggc tacctgatca
     7681 gcaacgtcaa cctgtaacga gcagcagcca tggcgaccaa aaagcctgct gcatttgggg
     7741 cgcctgtgaa aacggcccca gatgcgcagc agcgcaccga cacaccagcc tggcaggtgg
     7801 gcgcggtgcc ggtgtgccat gacggtgtgc gctatgcacc cggcgcggtg attgtgttga
     7861 ccagcgcaca ggcggcacgc ctggggctgc aacaagttgt caaggattcc ttgacatctg
     7921 gggctgcaac aagcccaagc ctgacaccca actgattaac tggagtaatt gaatgaagac
     7981 cgagaagatt ttgatggtga ccaccatact ggccgctgcg gcactggcgc gctttcggct
     8041 ggtggactac agcggcaacc caaccagcgc ggctgagcgg gcgctgggcg tggcggtgac
     8101 caactttgac accggcgagc aagccggtgt ggccgtcaag ggcgaggtgc tggtggaggc
     8161 cggtggcgca attgctgtgg gggccgaggt ggaaagcgat gcgagtagcc gcgtggtgac
     8221 caagaacgcc ggtgtcgtgg tgggggtggc gcgtgatgcg gctgcagcgg ccggtgacat
     8281 catccgcgtg ctgatctagc cccacagatg agccactaca gcaccattga cgatctggcc
     8341 caggccgcca ccaacggctg ggacgagctg gcccagcgcg ccggtggtgc gctggtggac
     8401 ggtgcgctgc tccaggccac cgcgctgctg gaagaccgta gcgcctggtc gccaatggcc
     8461 cgcgcccaag ccgacgcggc gctgctgcgc atcaccgcgc tgctggaggc ggccagccgc
     8521 catgcagata cctacctgtt cccccgctac cgcgcggtga tgccgctgac acctgagctg
     8581 gtgcaagcgt caagcctgcc gcaggcggtg gccgccattg cgctcaagcg cttgtatggg
     8641 gccagcctgc ctgatgacgt gcgcaatggc accaagtggg ccgacgacta cctgcgagac
     8701 ctgaataagg gtgtggtgag cctgggcgcg gtggacgata ccgtggccac gcccgccggg
     8761 cgcatggtga gccgggtgcc tgacaaggct tttgactggg acgggtactg agcatggatc
     8821 tcgacctgct ggccctggag actgcgctgc tggagcgcct gcgcacggcg ctggccggcc
     8881 agatgccgcc ggtgcatgtg cttagccagg acgatgtggc tgccgcggcc gaagaaaagc
     8941 aactggtgcc cgcagtgcat gtgatttacc aaaattaccg tgtgctggaa agccgcagcg
     9001 acggcaaggc agcgcgcatt gaacagattt ggctggccgt ggtggccacc cgcaacaccc
     9061 gcaacctggc atccgccacc agctcccgcc gccaggctgg cctgctgggt gggcaggtgg
     9121 cgaaggcgct gatgggctgg cgcgcaccgg gcaccgccac gccgtggcgg ctggtggatg
     9181 gccccggtgc ggcgtttggc actggctttg gctacctgcc aatagccgtt gccgccgaga
     9241 tgactgtgac cgcctgatat tgattaaccc aaaggacttt gcatcatgac cgacaccgtt
     9301 tactacccct accttggctc cggcaaaatc tacgcccgcg cctttggtgc cgccgctggc
     9361 ctgatggagc tgggcaacgc gagcaagctt gagctggccg tcaaggagga caagcaaaag
     9421 ctcaaggact acagcaagcc gggcgggggc ac
//
LOCUS       DAOSLL010000110         9438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_6970_length_9438_cov_6.026857, whole genome shotgun sequence.
ACCESSION   DAOSLL010000110 DAOSLL010000000
VERSION     DAOSLL010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9438
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6970_length_9438_cov_6.026857"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="PLB25_06635"
     CDS             complement(<1..776)
                     /locus_tag="PLB25_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOE41290.1"
                     /translation="MHSAVHDTIAAVWRIESAHIVAAVARRVRDLGVAEELAQDALVA
                     ALEHWPQHGVPNKPGAWLMSTAINRALDWLRHRQMADARHDAIASDLEAIQAHFVPDF
                     TDALDTAREHAAIGDDLLRLIFTACHPVLNRDARVALTLKLLGGLTTHEIARAYLVPE
                     ATIAQRIVRAKRFLADANVPFEVPRGEELNARLAAVLEVVYLVFNEGYTATSGSDWMR
                     PELCSEAMRLGRILAELMPQQSEAHGLVALMEIQASRTAAR"
     gene            complement(786..1196)
                     /locus_tag="PLB25_06640"
     CDS             complement(786..1196)
                     /locus_tag="PLB25_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HOE41291.1"
                     /translation="MFKQIFVNLPIKDMARSQAFFKALGLSFNPRFTNEQGACLEIGE
                     NFYAMLLVEPFFQGFTEKPISDAHKATEVLLALSVDSRAAVEEVIAKAVAAGAATPNA
                     PQDHGFMYQHGFADLDGHQWEVFWMDETAAPAQM"
     gene            complement(1239..1661)
                     /locus_tag="PLB25_06645"
     CDS             complement(1239..1661)
                     /locus_tag="PLB25_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006047341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HOE41292.1"
                     /translation="MRFMIIVKSCPAFEAELTPEAPEELMAEMAAYHDELMRAGVLLD
                     GSGLHPSRTGWRVHYGPEGKRIVDGPFAEAKELIAGYTLIQVRNREEALEWSRRFPNP
                     AGRGKEAVIELRQLIELDEFPPGPALDKFKQMEAEQKA"
     gene            complement(1681..2121)
                     /locus_tag="PLB25_06650"
     CDS             complement(1681..2121)
                     /locus_tag="PLB25_06650"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOE41293.1"
                     /translation="MSTVTVPMCAVVHFEMPYKDRQRAARFYAAAFGWTHQMLGPEMG
                     DYMLVSTAPPGARPPVPPDAALGSINGGLFPFKPDWPMQHPSVVISVDDIRAAMQRVK
                     AAGGEVMGEPMAIPGVGEYVVFRDTEGNHNSMMQPDMAPPACKT"
     gene            complement(2133..2576)
                     /locus_tag="PLB25_06655"
     CDS             complement(2133..2576)
                     /locus_tag="PLB25_06655"
                     /inference="COORDINATES: protein motif:HMM:NF019928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HOE41294.1"
                     /translation="MKTQHFDILIQAPRQQVWATMLQSPTYERWTTAFCEGSRFEGSW
                     DEGQKIRFLDPKGLGMVAEIAAHRPAEFVSIRHLGMIDQGVEDTTSEAVRAWVPCFEN
                     YTFTDEAGGTRLRVDCDDCGAGEDWMLQTWPKALQSLKTICENPT"
     gene            complement(2586..3062)
                     /locus_tag="PLB25_06660"
     CDS             complement(2586..3062)
                     /locus_tag="PLB25_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007175675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOE41295.1"
                     /translation="MKPRVHTCLWFDRQAEEVAQFYTTVFPNSRITEITRYSAGMPMP
                     EGTALTVSFELDGQTFTALNGDPQYTHSPAMSLVVTCTSQDELDRYWAALSASPEAEQ
                     CGWLVDRYGVSWQIVPQQVMDMLLHPDAAAKQRMMAALMPMKKLNIAALQNAFAAS"
     gene            complement(3206..3574)
                     /locus_tag="PLB25_06665"
     CDS             complement(3206..3574)
                     /locus_tag="PLB25_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HOE41296.1"
                     /translation="MPTYMLLIIEPPAQRATRTEGQGRAVYARMQDFGEGLRKQGKLL
                     AMESLASQASAVRVSLADGDSRVLDGPFSEAKEMVGGFFMIDCADQSEAVTIAQQCPA
                     AEWATVEVRSLAPCFDESSA"
     gene            complement(4351..5112)
                     /locus_tag="PLB25_06670"
     CDS             complement(4351..5112)
                     /locus_tag="PLB25_06670"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOE41297.1"
                     /translation="MNDLQLLIDLHLVAARQGPGGDAQTHLAIELSGLRNGRDLAIAD
                     IGCGTGASTLVLARELHAEVIAIDLMPEFLDALKQRAAQAGLADRITTETASMDSLPF
                     APGTLDAIWSEGAIYNIGFEKGVRDWRRLLKPGGIIAVSELTWLTDSRPAELEAHWQS
                     EYPEVDTASSKMAVLERNGFTPLGYFALPEHCWLDEYYRPMQKRFPIFLDAHKSSDAA
                     KALVAAEEHEIDLYERHRQCVSYGYYIARKTGDGS"
     gene            5535..6068
                     /locus_tag="PLB25_06675"
     CDS             5535..6068
                     /locus_tag="PLB25_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HOE41298.1"
                     /translation="MKCSHKNLMPGFSYPLSAQQLAVWNLLEEAPSNAALHLPVALQF
                     TGRLDVEAASSAIDQLIERHEALQMVFQNSPNGVRQHLGEEALPEPLLPMNLCDLPEE
                     ERQTEVNWLLIDRCEEAFDPDFGPMVRTALFQLDQGVPRPNRRNLSSFAKRNCYSQSI
                     PAKMASKSIGLPLSRNA"
     gene            complement(5999..9037)
                     /locus_tag="PLB25_06680"
     CDS             complement(5999..9037)
                     /locus_tag="PLB25_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007180430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HOE41299.1"
                     /translation="MRGDSNERLTILSDAEKTALYGIPSFNDFQRIEFFAMTDAERSL
                     ALQRRGILKQVYCLLQIGYFKAKQAFFQFSLDDVPPEDVAFLIQRYFPGKTLVVKQLS
                     TREYYAQRQEIGALFEYRLWTDNDLPVLRDKAKLLARTDVTPAFLLAELMVFLIGQHI
                     VRPGYSTLQTIIRDALSAERDRLEQLVEAAITDEARETLELLLANESTLSELAALKQD
                     AKSFRYRQMGIERQKRLTLKPLYAIAKKLLPGLALSQCNIAYYASLANFYTVYDLRRF
                     KPAKTNLYLLCYAWQRYRQLTDNVVEAFGYQTRQLEDETKAAAVQQATKLHVERQQAT
                     PRVGELLLLYVDDALPDVTPFGDIRHKAFRIMPADALRSTGKLLTERPVSQMDLRWQA
                     VEKQSGLCTKNLRPLATALEFASSSAGGKVWLAALKWMKEVFDRQESLAKQPLSDVPP
                     GALPKRLRDSLQIFGEDGMPLSLHGERYEFMVYRQLRKRLDAGDIYLDDSVQHRRFSD
                     DLVSMADKSEALKALDVPWLRKPVDETLDALFVELDDQWRAFDEELRAGKLKHLDFDA
                     DKKTLIWHRPKADKDKQLQKGFYAKLQARDIADIFRFVNERSHFLTALTPLQPRYAKK
                     IADEDSLMAVIIAQAMNHGNFKMAETCDITYHVLEATHQQHLRPATLIEANNKISNFI
                     ATLPIFPYYSIDMDVLYGSVDGQKFSAATPTSKARHSRKYFGKDRGVVAYTLLANHVA
                     LQTQMLGANQHESYWVFDICYNNTSDIVPNTITGDMHSINMANFAILHWFGMNLAPRF
                     TNLHAQLKHLYGGAGEEAYANFLIQPVGQIDRNLITSEKANMDQIAATLGLKEMSQSV
                     LMRKICTLSDHNRTRKAIFEFDKLVRTIYTLRYLRDPKLQRDVHRSENRIESYHQLRS
                     TIAQVNGKKELTGRTDLDVAVSNHCGRLIANVVIAYNSMLLSELLSRTLTAGNEKALE
                     LLRRISPAAWQHLHFLGHYAFRDKGKPIDLEAILAGIDWE"
     gene            complement(9044..>9438)
                     /locus_tag="PLB25_06685"
     CDS             complement(9044..>9438)
                     /locus_tag="PLB25_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOE41300.1"
                     /translation="RLGRSLSHLLSIVTALKDKQVAFRSLTEGMDTTTASGELLLHVF
                     GALAQYERALIQERVIAGLAAAKRRGRIGGRPAAIVGEKRDAIIAALDAGMSKAAVCR
                     NFGVKRTTLIETLARVGWPGAHDTKTRT"
BASE COUNT         1895 a         2612 c         2796 g         2135 t
ORIGIN      
        1 cgcgccgccg tgcgtgatgc ctgtatttcc atcaaggcga ccaagccatg cgcttcggac
       61 tgctgtggca tcagttcggc cagtatgcgg cccaggcgca tggcctctga gcagagttcg
      121 gggcgcatcc agtcactgcc actggtggcg gtgtagcctt cgttgaacac caggtaaacc
      181 acttcgagca ccgcggccag gcgagcgttc agttcctcgc cgcgtggcac ctcaaatggc
      241 acattcgcgt cagccaaaaa gcgcttggcg cgcacgattc gctgcgcgat ggtggcctcg
      301 ggcaccaagt aggcgcgagc gatttcatgg gtcgtgaggc caccaagcag cttgagcgtg
      361 agcgccacgc gtgcatcgcg attgagcacc gggtggcagg cggtgaagat caggcgcagc
      421 aggtcgtcgc cgatggccgc atgttcgcgg gcggtgtcca aagcgtctgt gaagtcgggc
      481 acgaaatggg cctggatcgc ttcgaggtcg ctggcaatgg cgtcatgccg tgcgtcggcc
      541 atctggcggt ggcgcagcca atccagtgcc cggttgatcg ccgtggacat gagccaggca
      601 ccgggcttgt tgggcacgcc gtgttgcggc cagtgttcaa gcgcggccac cagcgcgtcc
      661 tgcgccagtt cttcggccac gccgaggtcg cgcacacgcc gcgccacggc agcgacgatg
      721 tgtgctgact cgatgcgcca aacggcggca atggtgtcgt gaacagcaga atgcacgggg
      781 gcaggttaca tctgcgcggg cgctgcggtt tcgtccatcc agaacacctc ccactggtga
      841 ccgtcgagat cggcaaagcc gtgttggtac atgaaaccgt ggtcctgcgg cgcgttgggc
      901 gtggcggctc cagcggccac ggccttggcg atcacttctt cgactgcggc gcggctgtcg
      961 actgacagtg ccagcaggac ctctgtggct ttgtgtgcgt cgctgatggg cttttcggtg
     1021 aatccctgaa agaacggctc caccagcagc atggcgtaaa agttttcacc aatttccagg
     1081 caggcgcctt gctcattggt gaagcgcggg ttgaaagata ggcccagtgc cttgaagaag
     1141 gcttgtgaac gggccatgtc cttgattggc aagttaacga aaatttgttt gaacacggtt
     1201 ggttctcctc gttgtttgat gggttgcgtg ctggggaatc aggctttttg ctcggcttcc
     1261 atctgcttga acttgtccag cgccgggcca ggtgggaact catcgagctc aatgagctgg
     1321 cgcagttcaa tcaccgcctc cttgccgcgc ccggcggggt tgggaaaacg gcggctccat
     1381 tccagggctt cttcgcggtt gcgcacctgg atcagcgtgt aaccggcgat gagttccttg
     1441 gcctcggcaa acggcccatc cacgatgcgt ttgccctctg gcccatagtg cacgcgccag
     1501 ccggtgcggc tggggtgcag gccgctgcca tccagcagca caccggcgcg catcaactcg
     1561 tcgtggtagg cggccatttc ggccatcagt tcctcggggg cttcgggcgt gagctcggcc
     1621 tcgaaggcgg ggcaggactt gacgatgatc atgaatcgca tgggggttct ccttggtggc
     1681 tcaggtcttg caggcgggcg gtgccatgtc tggctgcatc atgctgttgt gattgccttc
     1741 ggtgtcgcga aacaccacgt actcgcccac gccaggaatc gccatgggct cacccatcac
     1801 ctcgccgcca gccgccttga cgcgttgcat ggctgcgcga atgtcgtcca cgctgatcac
     1861 caccgacggg tgttgcatcg gccagtctgg cttgaacggg aacagcccgc cgttgatcga
     1921 accgagcgcc gcgtctggcg gcacgggtgg gcgtgcgccc ggtggtgcgg tgctgaccag
     1981 catgtaatcg cccatctcgg ggccgagcat ctgatgggtc cagccgaaag cggcggcata
     2041 aaagcgggca gcgcgctggc ggtccttgta gggcatttca aaatgcacaa cggcacacat
     2101 ggggacggtg acagtgctca tgggattctc ctttaggtgg ggttttcgca gatggtcttg
     2161 agggactgca gcgccttggg ccaggtctgc agcatccagt cctcgccagc gccgcagtcg
     2221 tcgcagtcga cgcgcaagcg ggtgccacct gcttcgtcag tgaaggtgta gttttcaaag
     2281 caaggtaccc atgcgcgcac cgcttcgctg gtggtgtctt cgacaccctg gtcaatcatg
     2341 cccaggtgcc ggatcgagac gaactcggcc ggccggtggg ccgcaatctc tgccaccatg
     2401 cccaggccct tggggtcgag aaaacggatc ttctggcctt cgtcccagga accttcgaag
     2461 cgcgagcctt cgcagaaggc ggtggtccag cgctcgtagg tcggcgactg cagcatggtg
     2521 gcccacacct gctggcgcgg ggcctgaatg aggatgtcga aatgctgggt tttcatgggg
     2581 cggactcagg acgcggcgaa ggcgttttgc agtgccgcga tgttgagctt tttcatcggc
     2641 atcaaggccg ccatcattcg ttgtttggct gcggcgtcgg ggtgcaggag catgtccatc
     2701 acttgttgcg gcacgatctg ccaggacacg ccgtaccggt ccaccagcca gccgcattgt
     2761 tcggcctcgg gactggccga gagggcagcc caatagcggt ccagctcgtc ctgcgaggtg
     2821 caggtgacca ccagcgacat ggcaggcgag tgggtgtatt gagggtcacc attgagggcg
     2881 gtgaaggtct gtccgtcgag ctcaaaactg acggtcagcg ccgtgccttc gggcataggc
     2941 ataccggcgc tgtagcgggt gatttcggtg atgcgcgagt tcgggaacac cgtggtgtag
     3001 aactgggcga cttcttcggc ctggcggtca aaccacaggc aagtatggac gcgaggtttc
     3061 atgcaagaga ctcctggttg acaacgtggg cggcggcggg tttgccgtgc ctcgcgtgaa
     3121 gccgactcaa aggcttcaat gactacgacg aagcagcttg tgctttttcg acagcaccct
     3181 tctaggtaca aaccctgtta tcagatcaag ccgagctttc gtcgaaacag ggggcgagcg
     3241 agcgcacctc gaccgtggcc cactccgccg ctgggcattg ctgggcaatg gttaccgcct
     3301 cgctttggtc ggcgcaatcg atcatgaaaa agccgcccac catttccttg gcttcggaaa
     3361 agggcccgtc cagcacgcgg ctgtcgccgt ccgccagact cactcgcacg gcgctggcct
     3421 gcgaggccag cgactccatc gccagcagct tgccctgctt gcgaaggccc tcgccaaaat
     3481 cttgcattcg ggcgtagacc gcacggccct gcccttcagt gcgggtggca cgctgggcgg
     3541 gcggttcgat gatcaggagc atgtaggtgg gcatgtcgga cctttttctg tgcaaaggtg
     3601 gtcatcctac acatagcagg agttttgtgt gtgaggtggt ttggaattgt tttgcgctca
     3661 catctcgcgc aggtccagaa aacgtttcac tgcaatgccc gctctgtctt gtctgcaaag
     3721 gtcgtcaaaa gtggccggta tagggctggc aattcattgg tttgatcgac gggtaggggc
     3781 catctgcggt cgttcgagtt tgcgactgcc ctgactggat cagacccgaa gccgacgtac
     3841 accgggtcag catgaagccc gaagaaacgt cgaaagctga ccttcaattt cgccgcaagc
     3901 agactttcgt ttttgacacc cgaatctcag gagcggtcat tcaacggtga aaatcacctt
     3961 ttcaaactga cggtcagcac tgcagcggtt gcggtcgctc accgtgcaca aaaaaattga
     4021 tccaatttct ccaaagcggt cattgccggt gtcggtctac ttttgtcatt tgcccatagc
     4081 caatactctg aagcagtcat tctcgggcga caatcagttt ttgtacttgg cggtcggcac
     4141 tgcagcgtta gcgggtctag aaaattcaaa ttacccgccc aatacctgac attggtatta
     4201 ttggtattgg tagatcaatg gcagggagca gcggcaccga gatttcggcc aattgacagc
     4261 ccgttcccga cctgcaagag ttagccactg ctattccatc taatgacctc agcatggctg
     4321 ttatgctgag aggatggtca cgaaaagcct tcatgacccg tcgcctgttt tccgcgcaat
     4381 atagtagccg taactgacac actgcctgtg acgttcatag aggtcgatct cgtgttcttc
     4441 cgcagcaaca agggcttttg ctgcgtcgct tgatttgtgc gcatccagaa aaatcggaaa
     4501 acgcttttgc attggacggt aatattcatc aagccagcaa tgctctggta aggcaaagta
     4561 gcccaatggc gtgaagccgt ttcgttcgag gacggccatt ttggacgagg ccgtatcgac
     4621 ctctggatac tccgattgcc aatgcgcctc aagctcggca ggccgactat ctgtcagcca
     4681 ggtcaactca gaaacagcga tgatgccgcc cggcttgagc agccgacgcc agtcccgcac
     4741 acctttttcg aaaccgatgt tgtagatcgc accctccgac caaatagcat ccagcgtacc
     4801 cggtgcaaac gggagcgaat ccatggacgc tgtctcagtt gtgatgcggt cggccaaacc
     4861 agcttgggcg gcacgctgct taagtgcgtc cagaaattcc ggcataaggt cgattgcaat
     4921 gacttcggca tgaagctcac gtgccagaac cagggtcgat gcaccggtgc cgcaaccgat
     4981 gtctgcgatt gccaagtcac gcccgtttcg aagacccgat agctcgattg caaggtgggt
     5041 ctgtgcatca ccgccgggtc cttgtcgcgc ggccaccaag tgaagatcaa tcagcagttg
     5101 caagtcgttc atccatcatc cctctcgttt tcgcgatctt agcgacaagc gaagttcatt
     5161 gtttccaaat gattggacac tttgcagcac accacaaacg gcttctgtct aaattcaaac
     5221 cgggtagccc gtaagctgac tgtcatgacc ggcagcaaag ggtctttatg tagagcgccc
     5281 agttatgaat aggatttaat ggagcgaact actagattcc ataagtcggc aaagttgtta
     5341 tccaacaggc tgtcgctgtt tcgaagaaag tccgcggtca gctctggaaa caagcaggca
     5401 gttgtgtcat gattcaccgt ggcttttact attggtcagt agacgtttgc agaacaaccg
     5461 atttcgccct tataaaggct atttattgtt ttaaatcatg gacctacggt gttttttgaa
     5521 caccgagact tcagttgaaa tgttctcata agaatctgat gcctggtttt tcataccccc
     5581 tttcggctca acaactggct gtctggaatt tattggaaga agcaccttca aacgctgcac
     5641 tgcatctgcc tgtagcgctt caatttaccg gtcggttgga tgttgaggca gcgtccagcg
     5701 ccatcgatca gctaattgag cgccatgagg cgttgcagat ggtttttcag aattcgccga
     5761 atggtgtgcg acagcacctc ggagaagagg ctctccctga gccgcttctc cccatgaatc
     5821 tctgcgacct gccagaggaa gagcgccaga ccgaagtcaa ctggttgttg atagatcgat
     5881 gtgaggaagc gtttgatcca gactttggcc cgatggtgcg cacagcactt tttcagttgg
     5941 atcagggggt tccgcgccca aaccggcgga atttgtccag tttcgccaaa cgcaattgct
     6001 actcccagtc gatcccagcc aaaatggcct ccaaatcaat cggcttgccc ttgtcacgga
     6061 atgcgtaatg cccgaggaaa tgcaggtgct gccacgccgc tggcgaaatg cgtctgagca
     6121 actccagcgc tttctcgttg cccgctgtca gagtccggct aagcagttcc gacaacagca
     6181 tggagttgta agcgatcacg acgtttgcga tcaggcggcc gcagtggttg ctgacagcca
     6241 catccaagtc cgtgcgcccg gtaagttcct ttttgccgtt cacctgggcg atggtggagc
     6301 gtagctggtg gtaggactcg atgcggtttt ccgagcggtg cacgtcacgc tgcaacttgg
     6361 ggtcccgcaa gtagcgtagc gtgtaaatgg tacggaccag tttgtcaaat tcgaatatgg
     6421 ccttgcgcgt gcgattgtgg tccgacaggg tgcatatctt gcgcatcaac acgctttggc
     6481 tcatctcctt gagccccagc gtggcggcaa tctgatccat attggccttc tcggaggtga
     6541 tcaggttacg gtcaatttgg cccacaggct gaatgagaaa gttggcgtat gcctcctcgc
     6601 cagctcctcc gtagaggtgt ttcaactgcg catgaaggtt ggtgaaacgc ggggccaaat
     6661 tcatgccaaa ccagtgcagg atcgcaaagt tggccatgtt gatgctgtgc atgtcgccgg
     6721 tgatggtgtt tggcacaatg tcagaggtgt tgttgtagca gatgtcaaac acccagtagc
     6781 tctcatgctg gttggcaccc agcatctgtg tttgcaacgc cacgtggttg gccagcagcg
     6841 tgtaggccac cacgccgcgg tccttgccga aatatttgcg tgagtgccgt gcctttgacg
     6901 ttggggtggc ggcagaaaac ttctgcccgt caacgctgcc gtacagcaca tccatgtcga
     6961 tggagtaata cggaaagatc ggcagcgtgg cgatgaagtt actgatcttg ttgttcgctt
     7021 cgatcagtgt tgccgggcgc aaatgctgct gatgcgtggc ctccaggacg tggtacgtga
     7081 tgtcacaggt ctcggccatt ttgaagttgc cgtggttcat ggcctgagca atgatcaccg
     7141 ccatcaggct gtcttcgtcg gcgatctttt ttgcgtagcg cggttgcaag ggggtcaaag
     7201 ctgtcaggaa gtggctgcgc tcattcacaa atcggaagat atcggcaatg tctcgggcct
     7261 ggagcttggc atagaagccc ttttgaagct gcttgtcctt gtcggccttt ggccggtgcc
     7321 agatgagtgt tttcttgtca gcatcaaagt ccagatgttt aagcttgccc gcacgcagct
     7381 cctcatcaaa tgcgcgccac tgatcatcga gctcaacaaa gagggcatcc agtgtttcat
     7441 caactggctt tcgtagccat ggaacgtcga gcgctttgag ggcttcggat ttgtctgcca
     7501 tggacaccag gtcatctgaa aaccttctgt gctgcacgct gtcgtccaga tagatgtcgc
     7561 ccgcatcgag gcgtttacgc agttgcctgt acaccatgaa ttcgtaccgc tcgccatgca
     7621 ggctcagtgg catgccgtcc tcaccgaata tctgcaggga gtcgcgtaga cgtttgggaa
     7681 gtgcgcctgg tggcacgtca gagagaggct gtttggcaag ggattcctgg cggtcaaaca
     7741 cttccttcat ccatttgagc gccgcgagcc agactttgcc accggcactg ctgctggcga
     7801 attccaaggc agtggccagg gggcgcagat tcttggtaca aagcccgctt tgcttttcca
     7861 cggcctgcca gcgcaagtcc atctggctca caggtctttc tgtcagcagt ttgccagtcg
     7921 aacgtagggc atctgccggc atgatccgaa atgctttgtg gcgtatgtct ccgaagggcg
     7981 tgacgtcagg cagcgcgtca tccacataca gcaagagcag ctcgcccacc cgtggcgtgg
     8041 cttgttggcg ctcaacgtgt agtttggtgg cctgctggac ggccgccgcc ttggtttcat
     8101 cttccaactg gcgggtttgg taaccaaagg cttccaccac attgtcggtg agctggcggt
     8161 agcgttgcca tgcgtagcac agtaggtaca ggttggtttt agcaggcttg aatcggcgta
     8221 agtcgtagac ggtgtagaaa ttggccagac tggcgtagta cgcgatgttg cactgggaaa
     8281 gagcgagacc ggggagcagt tttttagcaa tcgcatacaa gggcttcagc gtgaggcgct
     8341 tttggcgctc tatgcccatc tggcggtagc gaaaactctt ggcatcttgt ttgagggcgg
     8401 ccaattccga caaggtgctc tcgttggcca gcagtagctc caatgtctcg cgtgcctcat
     8461 cggtaatggc cgcttcgacc aattgctcca gtcgatcacg ctccgcgctg agtgcatctc
     8521 tgatgatggt ttgcagcgtg gagtagcctg gtcgaacgat gtgctggccg atcaaaaaga
     8581 ccatcaattc ggccagcaaa aaggcagggg ttacatccgt tcgagccagc agtttcgcct
     8641 tatcgcgcaa caccggcaga tcattgtcag tccacaagcg atattcgaac aaggcgccaa
     8701 tttcttggcg ttgcgcgtaa tattctctgg tactcaattg ctttacaacg agcgttttcc
     8761 cagggaaata ccgctgaatc aggaaggcga catcctctgg cggcacgtca tccagggaga
     8821 actgaaaaaa tgcctgcttg gccttgaagt agccaatttg cagcaagcaa taaacctgtt
     8881 tcaagatgcc cctgcgctgc aaagccaagg agcgctccgc atcggtcatg gcaaaaaatt
     8941 caatacgctg aaaatcattg aaactgggaa tgccatagag agccgtcttc tctgcgtccg
     9001 agagaatggt cagtcgctcg ttgctgtcac cgcgcatatg tcattacgtt ctggtcttgg
     9061 tgtcgtgcgc acccggccat ccgacacggg ctaatgtttc aatcagcgtg gtacgtttga
     9121 caccaaaatt acggcacacg gcggccttcg acatgcctgc atccagcgcg gcaatgatgg
     9181 cgtcacgttt ctcgccgacg atggcagctg gtcggccacc gattcgacca cgccgcttag
     9241 cagcagccag accggcaata actcgctcct ggatcaaagc ccgttcgtac tgagcaagtg
     9301 caccaaacac atgaagcagc agttcgcccg atgccgtggt ggtgtccatg ccttcagtca
     9361 gcgaccgaaa ggcgacctgc ttgtccttca acgcggtcac gattgagagc agatgcgaca
     9421 gcgaacggcc aagtcgat
//
LOCUS       DAOSLL010000111         9360 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7033_length_9360_cov_4.650188, whole genome shotgun sequence.
ACCESSION   DAOSLL010000111 DAOSLL010000000
VERSION     DAOSLL010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9360
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7033_length_9360_cov_4.650188"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..1258)
                     /locus_tag="PLB25_06690"
     CDS             complement(170..1258)
                     /locus_tag="PLB25_06690"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HOE41301.1"
                     /translation="MDTAKPYSPFYRWLLLCLAVLLAHLGLLQSMPLTLSAGRSVADS
                     AMTFTTRAITLTPDPVPAPLTPTTVIRDKAAQPRKTVAKATPAATDITTADNHSPASS
                     FDGASDPLAAAPITAPSSAPELATAPEHTDQPTPPRPPPDLPHRFQAASLSGSTRLLY
                     KVQGNKFPFNLNGELNWQNQGQRYQARLSYSAFGQGRRQSSSGQITDAGLAPERFADK
                     YRSELAAHFNREHGKVTFSANTPDAPLLAGAQDRLSVLLQLGALVASEPERFGPGTTL
                     TVQTVGPREADLWLFTFEGSESLTLPGGTLEGIKLVRNPRKPHDQRVEIWLAPALGYL
                     PARIRITETNGDAMDQQWLSTEPVNVPP"
     gene            1289..2254
                     /locus_tag="PLB25_06695"
     CDS             1289..2254
                     /locus_tag="PLB25_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOE41302.1"
                     /translation="MKLASLNDGSRDGQLVVVSRDLVSAHYATGIADHLQQVLDDWNF
                     MSPQLQDLSDTLNQGKTRHAFAFDPRLCHAPLPRAHLLVAGDAFARFDAIARPADPTV
                     HLRLAFSDQLSGACQPMRCANARQGLDFEAALAVVTGDVAQAVTPEQALGGVRLLMLA
                     SHVQLRALQTASGEPLSSVPQTAFSPVAVTPDELVWGSDSAWVGGRLGLTLQCTVNGR
                     KLGLCDAGQDMRQHFGQLIAALCQTRRLHAGAIISSGEVRNHDSSRGSSSLAARRALE
                     LQQHGAAKTPWLGHGDSVRIEVKGRDGQSVFGAIEQQVLAADAGQ"
     gene            complement(2328..3545)
                     /locus_tag="PLB25_06700"
     CDS             complement(2328..3545)
                     /locus_tag="PLB25_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HOE41303.1"
                     /translation="MTTTEVFLLAMLIIFSVPWLIWRLCKTNHLAPLVVVQIITGIVL
                     GPGVLGHWFPDYHRFVFTAAVVQSLNGIAWWAVMLLVMMAGVELDLKQAWQHRRESSI
                     TAGLALGVPMLLGCAAAALLLKFDGWIGVQAQTWQFVLGIGMACAVTALPILILLLEQ
                     LDILHQPLGQRILRYASLDDIAIWSVLALILMDWTRIGRQLAFLPGFVLLGLLQRRLM
                     TRLAEADRWYVALIWLTLCALGSDWAGLHFMVGAFLAGAMLDADWFNKDQLDGLRRHV
                     LLVMMPVFFLSTGLRTNWAVGGAGVFAAAALLLLVSVAGKLLGVHLAARLLRWAPGDA
                     SVIGWLLQTKALVMIIFANILLDQQLITSDAFTALLLMAVGSTMLTVPVVSPKLRHAQ
                     ALAGLPPGAQPPA"
     gene            complement(3681..4100)
                     /locus_tag="PLB25_06705"
     CDS             complement(3681..4100)
                     /locus_tag="PLB25_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HOE41304.1"
                     /translation="MQIWQQPISVAALTGFSANTAVAHLGIEFLQVGDDFISARVPVD
                     ARTKQPFGLLHGGVSVVLAETLGSSAANFACQPGFQAVGLEVNANHLKGVRSGWVTGI
                     TRPIHIGRATQVWQIEMRNDAGELSCVSRITMAVLPA"
     gene            4243..4545
                     /locus_tag="PLB25_06710"
     CDS             4243..4545
                     /locus_tag="PLB25_06710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOE41305.1"
                     /translation="MIDGLIAGRLIGDPEQRSGKGGSPFVVARVRAQTSDGENLMVNL
                     IAFDAAPCRALLEMRDGDSVAAAGSLTPKVWTDKQGNVKPALDLVAHRVLTAPLDP"
     gene            4566..5204
                     /locus_tag="PLB25_06715"
     CDS             4566..5204
                     /locus_tag="PLB25_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE41306.1"
                     /translation="MFQYLTIPVTPFAQNCSLVWCDQTQQAAVIDPGGDLDQILGEVE
                     RLGLTLVQIWLTHGHIDHAGGTGELAQRLDLPIIGPQEADQFWIDGLPRQSQMYGFPP
                     ARPFKPTRWLHDGDTVTVGQCTLQVRHCPGHTPGHVVFHSVDAKRAFVGDVLFAGSIG
                     RTDFPKGDHDTLIASIKQRLWPMGDDTVFIPGHGPESSFGEERRYNPYVADR"
     gene            5356..5601
                     /locus_tag="PLB25_06720"
     CDS             5356..5601
                     /locus_tag="PLB25_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41307.1"
                     /translation="MDKDGQQVTSIRKASIDSSQGHTVGRYIDWLCGLMLVRRLPAWS
                     GNAGKRLVRAAKVRVPDRSQIHALPGLPDFHAVPGIR"
     gene            complement(6515..6649)
                     /locus_tag="PLB25_06725"
     CDS             complement(6515..6649)
                     /locus_tag="PLB25_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41308.1"
                     /translation="MYGRMRDGVTPVLNAVRLLLMVSSAVLALTMMRGPKGNASSHSD
                     "
     gene            complement(7017..8282)
                     /gene="ftsZ"
                     /locus_tag="PLB25_06730"
     CDS             complement(7017..8282)
                     /gene="ftsZ"
                     /locus_tag="PLB25_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HOE41309.1"
                     /translation="MSIELIDEEEFNQGTQIKVIGVGGGGGNAVAHMIASAVQGVEFI
                     CANTDAQALSTSDAHRVIQLGTSGLGAGSKPDKAREAAEIAIDHIREAIDGAHMLFIT
                     AGLGGGTGTGAAPVIARVAREMGILTVGVVTKPFEFEGKRRMDNADGGVAELEANVDS
                     LIVVLNEKLLEVLGDEATQDEAFAHANDVLKNAVGGIAEIINVPGHVNVDFEDVRTVM
                     GEPGKAMMGTAVAAGPDRARIAAEHAVACPLLEGIDLSGAKGVLVLITAAKGSLKLSE
                     SKLAMNTIRAYASPDAHVIYGTAYDDHLVDQIRVTVVATGLSRQQGVRPKLVVTQQPD
                     LKRTGTHDAPFEVSAQNGSNTGVPSGGMVSTAGGLGRSGQPDYGDLRTPSVWRRPHDR
                     TTAAAKVDALSSGGMDDFEIPAFLRKQAD"
     gene            complement(8424..>9360)
                     /gene="ftsA"
                     /locus_tag="PLB25_06735"
     CDS             complement(8424..>9360)
                     /gene="ftsA"
                     /locus_tag="PLB25_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOE41310.1"
                     /translation="KDREVTSADVSRVVETAKAINISTDQRLLLVEPQEFIIDGQDVK
                     EPIGMSGIRLEAKVHIVTGAQSAAENIIKCVRRCGLEVEQLMLNPLASSQSVLTEDER
                     ELGVALVDIGAGTTDLAIFTNGAIRHTAVIPIAGDLITSDIAMALRTPTKDAEEIKVE
                     NGFAKQLLADPEAQVEVPGLGDRGPRMLSRQALAGVIEPRVEEIFSLVHQVMRDSGFE
                     EMLSSGIVLTGGSCIMPGMVELGEDIFLKPVRRGIPKYTGALSDMVAQPRAATVMGLL
                     EDARMARLRGFKVAQKNGSMKTAFGSIKDWIVGNF"
BASE COUNT         1828 a         2872 c         2864 g         1796 t
ORIGIN      
        1 gccgtccttg agcgtgtccg ggcccacatc tgccgccacc gcgttggcca gcatgtgggt
       61 cacagaataa gtgtcagagt cgtaaagcgt gtgcattggc aaattatcca tgaatacaac
      121 atgccctgca gttgactgcc gattgggcaa attcaagggg tcagggaatt cacggtggca
      181 cattgactgg ctcggtcgat agccattgct ggtccatcgc atcgccattg gtctcggtga
      241 tgcggatgcg cgctggcagg tagcccagcg ccggtgccag ccagatttcc acccgctggt
      301 catggggttt gcgcgggttg cgcaccagtt tgatgccttc cagggtgccg ccgggcagcg
      361 tcagcgactc gctgccttca aaggtgaaca gccacaggtc ggcttcgcgt gggccgaccg
      421 tctgcaccgt gagtgtggtg cctggcccga aacgttcggg ctcgctggcc accagcgcac
      481 ccaactgcag cagcacgctg agccggtctt gcgcaccggc cagcaagggc gcatcgggcg
      541 tattggcgct gaaagtgacc ttgccatgtt cacggttgaa gtgggcggca agttcactgc
      601 ggtatttgtc ggcaaagcgc tcaggggcca gcccggcatc ggtgatctgg ccgctgctgc
      661 tctgcctgcg gccttggcca aaggcgctgt agctcagccg tgcctggtag cgctggccct
      721 ggttttgcca attcagctca ccgttcaggt taaacggaaa cttgttgccc tgtaccttgt
      781 acagcagtcg ggttgagccg ctcagactgg cggcttgaaa gcggtgcggc agatcaggtg
      841 gcggccgggg tggcgtaggc tgatcggtgt gttccggtgc ggttgccagc tcgggagctg
      901 aagacggtgc ggtgatcggt gctgctgcca gcgggtctga tgcgccgtcg aaactgctgg
      961 ctgggctgtg gttgtctgct gtggtgatgt cagtggccgc cggggttgct ttagccacgg
     1021 ttttgcgtgg ctgtgctgcc ttgtccctga tcacggtcgt gggggtcagc ggcgcgggca
     1081 cagggtccgg ggtaagggtg atggcgcggg tggtgaaggt catggcggag tcggccacac
     1141 tgcggcccgc gctcagggtc agcggcatcg attgcaacag gcccaggtgc gccagcagca
     1201 ctgccaggca gagcagcagc cagcgataaa acggcgaata tggctttgca gtgtccacta
     1261 aaatttgcag ccctgacgaa attgatcgat gaaacttgct agcttgaatg acggttcccg
     1321 cgatggccaa ttggtggtgg tgtcacgcga cctcgtcagc gcgcattatg cgactggcat
     1381 tgccgaccat ctgcagcagg tgcttgacga ctggaacttc atgtcgccgc agttgcagga
     1441 cttgtccgac accctgaacc agggcaagac gcgccatgct tttgcgtttg acccccgcct
     1501 gtgccacgcc ccgctgccgc gcgcccatct gctggtggca ggcgatgcgt ttgcccggtt
     1561 cgatgccatt gcccgaccgg ctgacccgac ggtccacttg cggctggctt tttccgacca
     1621 actgagcggc gcttgccagc cgatgcgctg tgccaatgcc cggcaagggc ttgattttga
     1681 agcggcgctg gcagtcgtga ccggcgacgt tgcgcaggct gtcacccccg agcaggcgct
     1741 gggcggggtt cgtctgctga tgctggccag ccatgtgcag ttgcgggcac tgcaaacagc
     1801 gtcgggtgag ccattgagca gcgtgccgca aaccgccttc agcccggtgg cggtgacacc
     1861 tgatgaactg gtgtggggca gcgacagtgc ctgggttggc ggcaggctgg gcttgaccct
     1921 gcaatgcacg gtgaatggcc gcaaactcgg tctttgcgat gccgggcagg acatgcgcca
     1981 gcattttggc cagttgatcg cagcactgtg tcagacccgc cgcctgcacg ctggtgccat
     2041 catcagttcg ggcgaagtcc gcaaccacga cagcagccgg ggcagcagca gcctggcggc
     2101 ccgccgggcg ctggagttgc agcagcatgg tgccgccaaa acgccctggc tcggtcacgg
     2161 cgacagcgtg cgcatcgagg tcaagggccg cgacggccaa agcgtgtttg gcgccattga
     2221 gcaacaggtg ctggcggcag atgccggcca gtaggcgttc gatttgaccc agccgatggc
     2281 agcgcctgca gttgctttga ctgccaggca cgggcgcaca tcgcctgtca cgcaggtggt
     2341 tgtgctccgg gtggcaggcc agccagcgcc tgggcgtggc gcagcttggg gctcaccact
     2401 ggcacggtca gcatggtgct gccgacggcc atcagcagca aggcggtgaa ggcatcgctg
     2461 gtgatgagct gctggtccag caggatgttg gcaaagatga tcatgaccag cgctttggtt
     2521 tgcagcaacc agccaatgac cgaagcgtca cccggtgccc agcgcagcag cctggcggcc
     2581 aggtgcacgc ccagcagctt gcccgccacc gacaccagca acagcagggc ggcagcagca
     2641 aacaccccgg caccgccaac cgcccagttg gtgcgcagcc cggtggagag aaagaacacc
     2701 ggcatcatca ccagcaacac atggcggcgc aagccgtcaa gctggtcctt gttgaaccaa
     2761 tcggcatcca gcatggcccc ggccaaaaag gccccaacca tgaaatgcag cccggcccag
     2821 tcggacccca gggcgcacaa ggtcagccag atcagcgcca cataccagcg gtcagcttcg
     2881 gccagcctgg tcatcagtcg gcgctgcagc aggcccagca gcacaaagcc cggcagaaag
     2941 gcgagttgcc tgccgatgcg cgtccagtcc atcaggatca gcgccagcac gctccagatg
     3001 gcgatgtcgt ccaggctggc gtagcgcaga atgcgctggc cgagcggctg gtgcaggatg
     3061 tcaagttgtt ccagcagcag aatcaaaatt ggcaaggcgg tcaccgcaca ggccatgccg
     3121 atgcccagca caaactgcca ggtctgcgcc tgcacgccga tccagccatc aaatttgagc
     3181 agcaacgccg ctgcggcaca acccagcagc atcggcacac ccagcgccag gccggcggtg
     3241 atgctgcttt cgcgccggtg ttgccaggcc tgcttcaggt ccagctcaac gccagccatc
     3301 atcaccagca acatcaccgc ccaccaggcg atgccgttga gtgactgcac cactgcggcg
     3361 gtaaagacaa accggtggta atccgggaac cagtgaccca gcacacccgg cccaagcaca
     3421 atgccagtga tgatttgcac caccaccagc ggggccaggt gattggtctt gcacaagcgc
     3481 cagatcaacc agggaacgct gaaaatgatc agcatcgcca gcaggaaaac ctcggtggtg
     3541 gtcacggcca aggtccgctg cgcaggtgga aaatgccagg cgttacagca ccgaccatag
     3601 cggcaaagcc ggcactgggc aaagcatcgt ggcgcagttc aacggtgtcg caccgcagct
     3661 aacgacagcc aatcgccaga tcaggcaggc aacacggcca tggtgatgcg cgacacacag
     3721 ctcagttcgc cagcatcgtt gcgcatttca atctgccaga cctgggtggc gcggccaatg
     3781 tggatgggcc gggtgatgcc ggttacccag ccgcttctga cacctttgag gtggttggcg
     3841 ttgacctcaa gcccgaccgc ctgaaagccc ggctggcagg cgaagttggc agcacttgag
     3901 cccagggttt cggccagcac cacgctgacg ccaccgtgca gcaggccaaa tggctgcttg
     3961 gtgcgtgcat cgaccggcac ccgggcgctg ataaagtcgt cgcctacctg caaaaattca
     4021 atgcccaggt gggcgaccgc cgtgttggca ctaaagcccg tcaatgccgc cacggaaatc
     4081 ggttgttgcc agatttgcat cagttgttct cagtcagtca aagtggcgtc actatatttc
     4141 aaaccgtctg ttgcaaacct tcgcgcagtt attggtgcca agagaccgct gcgccgctgc
     4201 gctaccatcc gggcttgatc gggaaccgga cttggagagc agatgattga tggtttgatt
     4261 gcaggccgac tgattggcga ccccgagcag cgttcaggca agggcggcag tccctttgtg
     4321 gttgccaggg tccgggcgca aaccagcgac ggggagaacc tgatggtcaa cctcattgct
     4381 tttgacgctg caccgtgccg ggccttgctg gagatgcgcg acggcgactc tgtggcggcg
     4441 gcgggtagcc tcacgcccaa ggtctggacc gacaagcaag gcaacgtcaa accagcgctt
     4501 gacctggttg cgcaccgggt tttgaccgcg cctttggacc cataaccgac aaataggaca
     4561 actgcatgtt tcaatacctg acgattcccg tgacaccgtt cgcacaaaat tgttcgctgg
     4621 tctggtgtga tcagacccaa caagctgccg tgattgatcc gggcggtgat ctcgaccaga
     4681 tactcggtga agttgagcgc ctgggcttga cgctggtgca aatctggctg acgcatgggc
     4741 atattgacca tgctggaggc accggtgaac tggcgcagcg gcttgacttg ccgatcatcg
     4801 gcccacaaga ggcggaccag ttctggattg acggtttgcc caggcagagt cagatgtatg
     4861 ggtttccccc ggccaggcca ttcaagccaa cgcgctggct gcacgatggc gacacggtca
     4921 cggttggcca atgcacgctg caggtgcggc attgccccgg tcacacgccg ggccatgtgg
     4981 tgtttcattc ggtagatgcc aaacgcgcct ttgtcggcga tgtgctgttt gccggctcga
     5041 ttggccgcac cgattttccg aagggcgacc acgacacttt gattgccagc atcaagcagc
     5101 ggctgtggcc aatgggcgac gacaccgtgt tcatccctgg ccacggcccg gaaagcagct
     5161 ttggcgaaga gcgccgctac aacccctatg tggccgaccg gtgatgctgc ataaaacata
     5221 gctggcagcg cttgttccat aagcgctgga ggccaatttg atgcatagaa agtgtgacag
     5281 ccagcgactg cctgggcaat gcagtagccc ggccagcgcc gctgctgcat cgcgcctggc
     5341 gtgcgcggga gccaggtgga taaagacggt cagcaggtca ccagcatcag gaaagccagc
     5401 attgacagca gccagggcca caccgttggc cgctacattg attggttgtg cggcctgatg
     5461 ctggtgcggc gtttgcccgc ctggagcggc aacgctggca aacggctggt gcgcgcagcc
     5521 aaggtgcgtg tgccagaccg tagccagatt cacgccttgc cgggcttgcc ggatttccat
     5581 gctgtgccgg gcatccggta gccgggttgg attgggttgg gttgggaatg ttgtgtgatt
     5641 gcgcaattgg tcgctgcggc accgctggtc cacgccagct tttgccgcac ccgccacgcg
     5701 gtcgaggtgg acttggtgct tgagatcaag cgccaatcga cacctatcga ctcctatcga
     5761 ctcctatcga cccctatcga ctcctatcga cccctatcgt ctccaagggc ttctggatta
     5821 ccgccaacga tgtcaaggcc agccgcaacc ggctggtggc ggcagttgac agcgcgttgg
     5881 tgatgcgcgt tggtgatgcg cgttggtgat gcgtgatggg ggtgacttga tgaatcggct
     5941 gctggtggcg atgcaggtgt agctgttgcc tcgcggttgt gttgccggtt cagaccctta
     6001 tgtatagcta tgctcaatgg cctcaagaag cccgtcatcc tcgattcaac aagcaaccta
     6061 actgctataa actaaagagc ttgcgacgca tatacaacgg gggctacagg ccaatttgcc
     6121 ctataaatgc caggcactcg gcactgttcc ggccatcacc atccgccaaa catccgtcaa
     6181 acacccgttg acgtacagct tacggtattc accctgccct tgcgcacaat caagggcctc
     6241 ggcgtagcag caccagctcg tcgcaccacc cagacaccag acaccaggcg cacagacccc
     6301 gtcgccacgg catgaaccgg ccgcaagaaa cagaactttt gactggcaat acatctcagc
     6361 gaccggctga gctgaggttc tccagaaaac tgacttgcga aatccgcctg ttgctgaccg
     6421 atcaaagcca aacttgccgg tggaatgtca gcgacgcagc atcaatgacg gacccttgac
     6481 gactcagaag tcgtcatcaa aaccagagcg ccggtcagtc agagtggctt gacgcattgc
     6541 cctttggtcc gcgcatcatc gtcagcgcca gcacggcaga gctgaccatc agcagcagcc
     6601 tcaccgcgtt caacaccggt gtgacaccgt cgcgcatgcg gccatacatg atcctagatt
     6661 ccagttggac gcgtagcgct gtcacccaat gggtgaactc catcgaagtt gaaaaagtca
     6721 atattgatga ggacttacag atgaaaacaa gcggtcaact gaatctaggt tcatacccgc
     6781 ttgtcggcct ggcgcacgag tgaggcacat cgaccactgg cgcgtggcca ttggttcaat
     6841 cgggtgcgtg gcgctgtcac ccaatgggtg aaccccaatc aggtcaaaac agcgaatgct
     6901 caaaaggccg ttcggaaagg agcaggcggc caattgagga attgaggttc aaacaacttg
     6961 cctggcgtgt cgcctggccg ggcggcccag ggccaacccc aatctgctat tacccctcaa
     7021 tcggcctgtt tgcgcaaaaa tgctggtatc tcgaagtcat ccatgccgcc gctggacagc
     7081 gcgtcgacct tggcggccgc cgtggtgcgg tcatgcggcc tgcgccatac gctcggcgtg
     7141 cgcaagtcgc cgtagtcagg ctgaccactg cgacccaaac caccggctgt gctgaccatg
     7201 ccaccagacg gcacgccggt gttcgaccca ttctgcgcag acacctcgaa cggtgcgtca
     7261 tgggtgccgg tgcgtttcaa atcgggttgt tgggtcacca ccaatttggg ccgtacaccc
     7321 tgctgacggc tcaggccggt ggccaccaca gtgacgcgaa tctggtcgac caaatggtcg
     7381 tcataggcgg tgccgtagat cacatgcgca tcgggtgagg cataggcgcg gatggtgttc
     7441 atcgccagct tggactcgct cagcttcaaa gaccccttgg cggcggtgat caataccagc
     7501 acacccttgg cacctgacag gtcaatgcct tcgagcaacg ggcaagccac cgcatgttcg
     7561 gccgcaatgc gggcacggtc cggcccggcc gccacggcag tacccatcat ggccttgcca
     7621 ggctcgccca tcactgtgcg cacgtcttca aagtcgacgt tcacgtggcc cggcacattg
     7681 atgatttcag caatgccacc gacggcattt ttcagcacat cattggcatg cgcaaaggcc
     7741 tcgtcctgcg tggcttcgtc gcccagcact tcgagcagct tttcgttgag taccacgatc
     7801 agcgagtcaa cattggcttc cagctcggcc acgccgccgt cggcgttgtc catccgccgt
     7861 ttgccttcaa actcaaaagg cttggtcacc acgccgaccg tcaggatacc catttcacgt
     7921 gccacgcggg caatcacggg cgcggcacca gtgccggtgc cgccacccag gccggcggtg
     7981 atgaacagca tgtgtgcacc atcaatggcc tcacggatgt ggtcaatggc gatttctgcc
     8041 gcctcacgcg ccttgtcggg cttgctaccg gcacccagtc cactggtgcc gagctgaatc
     8101 acgcgatgcg cgtcgctggt cgacagtgcc tgtgcgtcgg tgttggcaca gatgaactcc
     8161 accccctgca cggcgctggc gatcatgtgg gccacggcgt tgccgccacc gccgccgacc
     8221 ccaatgacct tgatctgggt gccttggttg aattcttcct cgtcgataag ttcaatggac
     8281 atggtgcgct cctggtatgt caaatggtgg gtaaaaactg atagggggtg tggtcggaaa
     8341 ttcaaaatgc tttgccggcg ggtcaacaca tcgccttcgt cgcggcgggc cagcacggct
     8401 taaccaggtg tgggttttcg tcatcagaag ttcccgacaa tccagtcctt gatgctgcca
     8461 aaggcagttt tcatcgaacc gtttttttgc gccaccttga agccacgcaa acgcgccatg
     8521 cgggcatctt ccagcaagcc catgacggtg gcggcacgtg gctgggccac catgtcggac
     8581 agcgctccgg tgtacttcgg aatgccccgg cgcaccggtt tcagaaagat gtcttcaccc
     8641 agctcgacca tgccgggcat gatgcaactg ccacccgtga gcacaatgcc gcttgacagc
     8701 atttcttcaa agccggaatc gcgcatcact tggtgcacca gactgaagat ctcttccaca
     8761 cgcggctcaa tcacaccggc cagcgcctga cggctcagca tgcgcgggcc acggtcgccc
     8821 agacccggca cctcgacctg cgcttccggg tcagccagca actgcttggc gaaaccgttt
     8881 tcgaccttga tttcttcggc atccttggtt ggcgtgcgca atgccatcgc gatgtcgctg
     8941 gtgatcaggt cgccggcaat cgggatcacc gccgtgtggc gaatcgcgcc attggtgaaa
     9001 atcgccaggt cggtagtgcc agcaccaatg tccaccaatg ccaccccgag ttcacgctcg
     9061 tcttcggtca gcaccgactg gctcgaagcc agcgggttaa gcatcagttg ctcgacttcc
     9121 agaccacagc gccggacaca cttgatgatg ttctcggccg cgctctgcgc cccggtgacg
     9181 atgtgcacct tggcttcgag ccggatgcca ctcatgccaa tcggctcttt cacgtcctgc
     9241 ccgtcgatga tgaattcctg cggctccacc agcagcaggc gttgatcggt cgagatattg
     9301 atggccttgg cggtttccac cacccgcgac acgtcagcgg aggtgacttc gcggtccttg
//
LOCUS       DAOSLL010000112         9307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7071_length_9307_cov_4.241677, whole genome shotgun sequence.
ACCESSION   DAOSLL010000112 DAOSLL010000000
VERSION     DAOSLL010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9307
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7071_length_9307_cov_4.241677"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..409)
                     /gene="minE"
                     /locus_tag="PLB25_06740"
     CDS             complement(149..409)
                     /gene="minE"
                     /locus_tag="PLB25_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HOE41311.1"
                     /translation="MSFFSFFLGEKKKTASVAKERLQIILAHERSGRNPTTPDYLPDL
                     QRELIAVISKYVKINPEDIKVNLERQDNLEVLEVKIELPDKR"
     gene            complement(413..1228)
                     /gene="minD"
                     /locus_tag="PLB25_06745"
     CDS             complement(413..1228)
                     /gene="minD"
                     /locus_tag="PLB25_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HOE41312.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASFATGLAMRGHKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEANLNQALIKDKQCDKLYVLAASQTRDKDALTQSGV
                     EKVLADLAAMDFEYIVCDSPAGIETGAMMAMHFADEALVVTNPEVSSVRDSDRILGML
                     SSKTKRAMEGQTPITEHLLITRYNPARVNQGQMLSLQDIQDILRIKLIGVIPESETVL
                     QASNQGVPAVHLEGSDVSEAYKDVISRFLGEDKPMRFTEPPKQGLFKRLFGGK"
     gene            complement(1256..2029)
                     /gene="minC"
                     /locus_tag="PLB25_06750"
     CDS             complement(1256..2029)
                     /gene="minC"
                     /locus_tag="PLB25_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HOE41313.1"
                     /translation="MVNSFEIKSAQLALVSLVLKSANLQQVGLDLLQQYGPDSDSADF
                     FDHDALVLDFSRLDSPVALPDVKKLLKQLPACHLKPVAFRGCQTTDVATLKTLGLVEA
                     PPDVVRHKAAPESTALAPAPTETVHEVVREVIREVSVEVPGPATLIIDRQVRSGQKIY
                     ARAADLVVLAMVNQGAEVVADGNIHVYAPLRGKAMAGASGNTRARIFSMCLEPELISI
                     AGVYRTSENPLASDVQGKPAQVRLSDDGQENLLFEALNH"
     gene            complement(2543..3931)
                     /gene="ccmI"
                     /locus_tag="PLB25_06755"
     CDS             complement(2543..3931)
                     /gene="ccmI"
                     /locus_tag="PLB25_06755"
                     /inference="COORDINATES: protein motif:HMM:TIGR03142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HOE41314.1"
                     /translation="MNAFIGIAALLTLLVVAWLVRPLLRSTTPSGVSSERLNVAVHRD
                     QLQALEADLARGAISQQDFETTRDELQLRLLDDTQGFTALPTQNRNGFWSARLTAVAI
                     ALGLPPLALGIYLQLGTPGAINPVATAKVNEQQIRQMIDTLAARLQANPDNPKGWAML
                     ARSYKVMGRLAEARQAFEKAGELLNTDPDLLVDYADVLASQAQGNLEGRPLELVNRAL
                     SINPQHALGLMISGVAAYRRSDFKLAINQWNKLLPLLEPGSPDAQQIEADIADARAKA
                     DTPTAPNSQQMAAPAKPADTATDTGKLPPVPAGAPVGMTPEMINQMVDRLAARLKDNP
                     DDVAGWARLARAYKVQGRSDDALQAYGKTGRLLDTDADLLTQYADLLATRAKGDFKGK
                     PQALIDKALGINPRHPMALMLAGHAAYSAANYSKAIRHWETVLTVLAPDSPDATQVKA
                     EIADALAKLAKK"
     gene            complement(3928..4395)
                     /locus_tag="PLB25_06760"
     CDS             complement(3928..4395)
                     /locus_tag="PLB25_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HOE41315.1"
                     /translation="MTYWLTLFLALLCSFATYANEAPPLAEDPVVEQRLVVIAEELRC
                     LVCQNESLAGSRAELAMDLRREVRSLIKSGKTDTEIKEFLVSRYGDFILYRPPVKPST
                     WLLWFGPLLLLLAAVWILIGIVRRNQQQKDMPVIDEQQRARAQALLQETDSRK"
     gene            complement(4392..4916)
                     /locus_tag="PLB25_06765"
     CDS             complement(4392..4916)
                     /locus_tag="PLB25_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HOE41316.1"
                     /translation="MKKTLIPLLIFVVLVIFLAIGLTRDPREIPSPLIGKPAPGFTAP
                     VLGGAGLMFSDKDMLGKVWLLNTWASWCVACRQEHPLLIELARTKLLPIVGLDYKDKD
                     ADGLKWISRYGNPYDVSIADRDGRIGIDFGVYGVPESFLIDKLGIIRYKQIGPFTEEA
                     IRDKLLPLVRELQR"
     gene            complement(4913..6928)
                     /locus_tag="PLB25_06770"
     CDS             complement(4913..6928)
                     /locus_tag="PLB25_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HOE41317.1"
                     /translation="MIPELGSFALILALLVSFCLGVIGVAGGQQNRADWMAFARPGAQ
                     ALWLLTAFSFACLTYAFYTNDFSVLYVSQHSNSKLPDMYRIAGVWGGHEGSLLLWLMM
                     LSTWMMAVSIFSRQLPDVMVSRVLGVLGLVAMGFLLFMLITSNPFLRLSDIPEDGRDL
                     NPLLQDPGLVFHPPLLYMGYVGMAIPFAFAIAALLNGRLDAAWARWSRPWATAAWIFL
                     TVGIALGSWWAYYELGWGGWWFWDPVENASFLPWLVSTALIHSLAVTEKRGLFRNWTI
                     ILAITSFSLSLLGTFLVRSGVLTSVHAFATDPRRGVFILVFLALVVGGSLILFALRAS
                     KVRAGGNFALVSRETFLLVNNVLLTTAAASVMLGTLYPLIVDALNLGKLSVGPPYFNA
                     VFAPIMVPVLLFMVIGSYARWKSDSVAELARKLRPVAIISVALGCSLPLLAGPWSPIV
                     AVGLSLSVWIMLASAVQIYRHVKALAPGQTTPLAYWGMHVAHIGIAVCVIGITMVKGY
                     ETEKDVRMTLGDTVSVGGYTFRLTGISERRGPNYNANVGSVELLQDGKLLKTLHPEKR
                     TYFSSTMPMTEAAIDSGLTRDVYVSLGEKLDDGPNAAWAMRVYHKPFVTWIWVGCMIM
                     ALGGGMAALDRRYRKKLAQDTKSGMTNNTQVDAAGKPPKGNAQTIPA"
     gene            complement(6941..7378)
                     /gene="ccmE"
                     /locus_tag="PLB25_06775"
     CDS             complement(6941..7378)
                     /gene="ccmE"
                     /locus_tag="PLB25_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HOE41318.1"
                     /translation="MKPRHKRAAIIAGGLVAIGLAAYFVLNAFESNLVFFFSPSQIES
                     GEAPRNRTFRVGGLVKSGSVNRDNLTVNFVVTDTAKEIRVSYTGILPDLFREGKGVVA
                     QGKLDASGHFTATEVLAKHDENYMPPEAKHAVDQAQNTIKTLK"
     gene            complement(7375..7533)
                     /gene="ccmD"
                     /locus_tag="PLB25_06780"
     CDS             complement(7375..7533)
                     /gene="ccmD"
                     /locus_tag="PLB25_06780"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HOE41319.1"
                     /translation="MGGYAFYVWGSFGATAAVIAAEIWQVRSHRRQILRNLISEIESQ
                     PDATLHSS"
     gene            complement(7553..8290)
                     /gene="ccmC"
                     /locus_tag="PLB25_06785"
     CDS             complement(7553..8290)
                     /gene="ccmC"
                     /locus_tag="PLB25_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HOE41320.1"
                     /translation="MKTPVIHWFKFASPQNFYHLAGKMWPWFAALATLLALVGLWIAL
                     FVAPIDAQQGQGYRIIFVHVPASQMAMFIYLVMAAWAGLGLAFNTRLSGMMASALAPT
                     GALFAFLSLLTGALWGKPMWGAWWVWDARLTSTLILLFLYLGFMALQASIDDPRRADK
                     AGAVLALVGAVNVPIIYFSVKWWNTLHQGASVSLKGSSMASTMLAGMLIMVLAFWMYA
                     IAMALLRVRNIILEREHHAEWVKHAVA"
     gene            complement(8287..8982)
                     /gene="ccmB"
                     /locus_tag="PLB25_06790"
     CDS             complement(8287..8982)
                     /gene="ccmB"
                     /locus_tag="PLB25_06790"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003149819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HOE41321.1"
                     /translation="MSQTVKSIGLIHVMLAVLKREVALAMRQKGEVLTPLVFFMVIAS
                     LFPLGVGAESSLLLRMAPGVLWVSALLAAMLSLQRMFATDYADGSLEQMVLSPTPLGL
                     LVLAKALAHFVLSGLPLVLMAPVLGLQFGLDARALAILMLTLLLGAPSLSLIGSIGAA
                     LTLGVRGAGVLLSLLILPLYIPVLIFGAGAVEADAAGVGFGGHLSLLSALLVLSLFFS
                     PFATAAALRISLE"
     gene            complement(8979..>9307)
                     /locus_tag="PLB25_06795"
     CDS             complement(8979..>9307)
                     /locus_tag="PLB25_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE41322.1"
                     /translation="SALGGIAPDHQQSQEVLARFGMRGRSQQLVRHLSQGQKRRVALS
                     RLALSPARLWVLDEPYVAMDEGGIQLLSDLIAHHLDQGGLAVLTSHQRVPIGQHPAQM
                     LELKAP"
BASE COUNT         2183 a         2609 c         2594 g         1921 t
ORIGIN      
        1 gaacttttcg caacaactca tcacagcaac ggggtaactg aggaatttag gttgatgact
       61 tgttccttga tcatgatggc tccgtttcaa acaggctcat atgcctgctt ttgtgggtct
      121 gattttaggc ctcgccgatg cgcaatacct atcgtttgtc tggcaattcg atcttgactt
      181 caagcacttc caggttgtcc tggcgttcca gattgacttt gatgtcttcc ggattgatct
      241 tgacgtactt gctgatgacg gcaatcagtt cacgctgcaa atcaggcaga tagtctggcg
      301 tagtcgggtt gcggccgctt cgctcatgcg ccaggatgat ctgtaagcgt tctttggcaa
      361 cgctcgcggt tttctttttc tcgcccagaa aaaatgaaaa aaacgacatg gcttatttcc
      421 ccccgaacaa gcgcttgaac aagccctgtt tgggtggctc ggtaaaccgc atcggtttgt
      481 cctcgccgag aaaacggctg atcacgtcct tgtaggcctc agaaacatca ctgccctcaa
      541 ggtggaccgc aggcacacct tggttggagg cctgcagcac cgtttcgctc tcgggaatca
      601 cgccaatgag cttgatgcgc aagatgtcct gaatgtcctg cagcgacaac atctgaccct
      661 ggttgacccg cgccgggttg taacgggtga tcagcaaatg ttccgtgatc ggggtctggc
      721 cttccatggc gcgcttggtc ttgcttgaca gcatgcccaa aattcgatcc gagtcacgca
      781 ccgatgacac ctcggggttg gtcacgacca gcgcctcatc agcaaaatgc atggccatca
      841 tggcaccggt ttcgatcccg gccggtgaat cacaaacaat gtactcaaag tccatcgctg
      901 ccaggtcggc cagcaccttc tcaacgccac tctgggtcag ggcatccttg tcgcgcgtct
      961 gggaggccgc cagcacatac agcttgtcac attgtttgtc cttgatcaac gcctggttca
     1021 ggttggcttc gccttgaatc acattgatca ggtcatacac cacacggcgc tcgcaaccca
     1081 tgatcagatc aaggttgcgc agcccaacat caaagtcaat cacggcggtt ttgtgaccac
     1141 gcatggccag gccggttgca aaactggcac tggtggtggt tttacccacc ccccctttgc
     1201 cagaggtcac cacgataatt tttgccatgc tgggagaatc tttcaaaaaa tgaactcaat
     1261 ggttcaaggc ttcaaacaac aggttttcct gaccatcatc gctcaggcgc acttgcgccg
     1321 gtttgccttg aacatcggat gccaaaggat tttcgctggt tcggtagacc ccggcgatgg
     1381 aaatcagctc aggctcaaga cacattgaaa aaatacgtgc ccgggtgttg ccactggcac
     1441 ctgccatcgc cttgccacgc agtggggcat aaacgtgaat attgccgtcc gccaccactt
     1501 cagcaccctg gttgaccatc gccagaacca ccaggtcagc cgcgcgagcg taaatttttt
     1561 ggcctgatcg aacttgtctg tcaatgatca gggtagctgg gccaggcacc tctaccgata
     1621 cttccctgat cacttcgcgg acgacttcat gcactgtttc tgtcggagca ggggcaagcg
     1681 cggtactttc cggtgcggcc ttgtgacgca ctacatcggg tggcgcttca accaggccca
     1741 gcgttttaag ggtcgctaca tcggtggtct gacatccccg aaatgcaacc ggcttgaggt
     1801 gacaggccgg gagctgtttc agcagctttt tgacatctgg caacgcaacc ggggaatcaa
     1861 gccgggaaaa atccagcacc aaagcgtcat ggtcgaaaaa atcagcgcta tcgctgtcag
     1921 gtccgtattg ctgcagcaga tcaagtccga cttgctgcaa attggcggat ttcagtacca
     1981 gcgacaccaa agctaactgg gcacttttga tttcgaatga atttaccacg tgacctgatc
     2041 atgaaacggt tgaaaaaagt gcgggctgaa agcgtactga agcaccccta aatcttactt
     2101 gattgtgctg gaagatcctt ctcaaaaatc atgtttattc ggcttatgca cagcattttc
     2161 aaaatatttc gaagtgcttt gcatttgacc tgaaatttgt gttcctgacg atctttgtat
     2221 ttttatatga atgaaatagg gttagtagta aggattgaga tgaatgtgga gaagtcactt
     2281 aatgtgatta aattcaacaa cttgtctgtt gtcataggct gtgtgtgagt ctgcttttct
     2341 ggtgtcgtgc aaacctgaac aagttgtgca attgaaaaat ttgtgtggat aagtcaccga
     2401 aaactgatta attgttaaca gctttataca ctgacattcg ggcacctctt gatgagcctg
     2461 caagtcgcca gaaagcaaaa aagccagacc ggtttgaccc gagtctggct agcaggcact
     2521 gcaaagagcc gactatcgat gcctatttct tcgcaagttt ggcgagcgca tcggcaattt
     2581 cagccttcac ctgggttgca tccggcgaat caggcgcaag cactgtcaac acagtttccc
     2641 agtgtctgat ggccttgctg taattggccg cgctgtaagc cgcatgaccg gccagcatga
     2701 gagccatcgg gtgcctggga ttgatgccaa gcgccttgtc aatcagggcc tgtggtttgc
     2761 ccttgaaatc gcctttggcg cgtgtcgcca gcaggtcggc gtactgggtc agcaggtcgg
     2821 catcggtgtc gagcagccga cccgtcttgc cataagcctg caatgcgtcg tcgcttcggc
     2881 cttgaacctt gtaggcgcgg gccagccgtg cccagccagc gacatcgtcc gggttgtctt
     2941 tcaggcgggc tgccagccgg tcaaccatct ggttgatcat ctcgggtgtc atgccgacgg
     3001 gggcacctgc gggcacgggt ggcagtttgc cggtgtcggt ggcagtgtcg gcgggtttgg
     3061 ctggcgctgc catctgttgt gagttcggtg ccgttggtgt gtcggccttg gcacgcgcat
     3121 cagcgatatc ggcttcaatc tgttgcgcat ccggtgaacc gggctccagc aagggcagca
     3181 gcttgttcca ttggttgatg gcgagtttga agtcagagcg gcgataggct gcaacgccgg
     3241 aaatcatcaa cccaagcgca tgctgcggat tgatgctcaa ggcccggttg accagctcaa
     3301 gcggtcggcc ctccaggttg ccttgcgcct gactggccag cacatcggcg taatcgacca
     3361 gtaaatcagg gtctgtgttg agcagttcac ccgccttctc aaacgcctgg cgggcttcgg
     3421 caagtcgtcc catgactttg taggacctgg ccagcattgc ccagcccttt gggttgtccg
     3481 ggttggcctg caggcgtgct gccagggtat cgatcatttg ccggatttgc tgttcattga
     3541 ccttcgcagt tgcaaccggg ttgatggcac ccggtgtgcc aagttgcaga taaatgccca
     3601 gtgccagtgg cggtaggccc agtgcgatgg caacggcggt aagcctggct gaccagaatc
     3661 cgttgcgatt ttgggttggc agtgcagtga aaccctgggt gtcatcaagc aagcgcagtt
     3721 gcagttcgtc gcgcgttgtc tcgaagtcct gttggctgat ggccccgcgc gccaagtctg
     3781 cttccagcgc ctgaagttga tcacggtgga cagcgacatt gagccgttcg ctggaaacac
     3841 cagaaggggt tgttgagcgc aacaatggtc gcaccagcca ggctaccacc agcagcgtca
     3901 acaacgctgc aatgccaata aatgcgttca ttttctggaa tcagtctctt gaagcagggc
     3961 ttgtgccctg gcgcgctgct gctcgtcgat gaccggcatg tctttttgct gttggttgcg
     4021 ccgaacaatg ccgatcaaaa tccagacagc agccagcagc agcagcagcg gaccaaacca
     4081 tagcagccag gtgcttggtt tgacgggagg gcggtacagg ataaagtcac cgtaacgact
     4141 gaccagaaat tccttgattt cggtatcggt tttgccggac ttgatcaatg agcggacttc
     4201 acggcgcaag tccatggcca actcggcacg cgagcccgcc agcgattcgt tttgacacac
     4261 caggcaccgc agttcttcgg caatgacgac caggcgttgt tccaccactg ggtcttcggc
     4321 caggggcggg gcttcgttgg cataggtggc aaagctgcat aggagtgcca aaaaaagggt
     4381 cagccaatat gtcatctttg cagttctcgc accagaggga gcaatttgtc tctaatggcc
     4441 tcttctgtga aaggaccaat ttgcttgtaa cggatgatcc ccagcttgtc gatcaggaag
     4501 ctttcaggaa cgccatagac accaaaatca atgccgatac ggccatctcg gtcagcaatg
     4561 gacacgtcat aggggttgcc atagcggctg atccatttga gaccatcagc gtccttgtcc
     4621 ttgtaatcta gtccgacgat gggcagcagt ttggtgcggg ccaactcgat cagtagcgga
     4681 tgttcctggc gacaggcgac acaccaggag gcccaggtgt tgagcagcca aaccttgccg
     4741 agcatgtctt tgtcggagaa catcaagccg gcaccaccaa gcaccggtgc ggtaaagcct
     4801 ggcgctggtt tgccgatcag cggggaggga atttcacgtg ggtcacgtgt cagcccgatg
     4861 gccagaaaaa tcaccagaac gacaaaaatc agcagcggaa tcagtgtctt tttcatgccg
     4921 gaatggtttg tgcattgccc ttgggcggtt tgccagcggc atcaacctgg gtgttgttgg
     4981 tcatgccgga tttggtgtct tgtgccagtt tcttgcggta acgccggtcc agtgccgcca
     5041 tgccaccgcc taatgccatg atcatgcagc cgacccagat ccaggtgaca aagggtttgt
     5101 gatagacccg catggcccag gcagcgttgg ggccatcgtc aagtttttct cccagcgaca
     5161 catagacatc ccgtgtcagg ccgctgtcaa ttgcagcctc ggtcattggc atggtggatg
     5221 aaaaataagt gcgcttttca gggtgcaggg ttttcagcag tttgccatcc tgcagcaact
     5281 cgaccgagcc aacattggcg ttgtaattgg gcccgcggcg ctcgctgatg ccagtcagtc
     5341 gaaaggtata gccaccgaca gaaaccgtgt cacccagggt catgcgcaca tctttttcag
     5401 tctcgtagcc tttgaccatg gtgatgccaa tcacacacac ggcgatgcca atgtgcgcca
     5461 catgcatgcc ccagtacgcc aacggggtgg tctggcccgg ggccagggct ttgacatgcc
     5521 ggtaaatctg aaccgccgag gcgagcatga tccagaccga caagctcaga ccgacggcca
     5581 ctatgggcga ccagggaccg gcaagcaatg gcaagctgca acccagagcc accgaaatga
     5641 ttgcaaccgg acgcagtttc ctggccagct cagcgacgct gtcgcttttc cagcgggcat
     5701 aagagccgat caccatgaac aacaacaccg gtaccatgat gggcgcaaac accgcgttga
     5761 agtaaggcgg gccgaccgac agtttgccaa gattgagcgc gtcgacgatt aacgggtaca
     5821 gggtgcccag catgacgctg gctgccgctg tggtcagcag cacattgttg accagtagaa
     5881 aggtttcacg tgaaaccagg gcaaagttgc cgcctgcgcg gactttgctg gcgcgcagtg
     5941 caaacaggat cagcgaccca cctacgacga gcgccagaaa caccaggatg aacacgccgc
     6001 gcctggggtc agtggcaaaa gcgtgaaccg aagtcagaac accagagcgc accagaaaag
     6061 tgcccagcag actcagtgaa aacgatgtaa tggcgagaat gatggtccag tttctgaaca
     6121 agccgcgctt ttcggtgacg gcgagagagt ggatcagcgc ggtactgacc aaccagggca
     6181 ggaacgacgc gttctcgacc gggtcccaaa accaccagcc accccagccc agttcgtaat
     6241 aggcccacca ggagcccaag gcgatgccga ctgtcaggaa tatccaggct gcagtggccc
     6301 aagggcgcga ccagcgggcc caggccgcgt cgagccgacc attgagcaag gcggcaatgg
     6361 caaacgcaaa cggaatcgcc atgccgacat aacccatgta aagcagcggc gggtgaaaca
     6421 ccagaccggg gtcttgcagc agcggattga ggtcgcgacc gtcttctgga atgtcactca
     6481 ggcgcagaaa gggattggaa gtgatcaaca tgaaaagcag aaagcccatc gccaccagac
     6541 ccagcacacc cagtacccgc gaaaccatga catcaggcaa ctggcgcgaa aaaattgaca
     6601 ccgccatcat ccaggtgctg agcatcatca gccagagcag cagcgagccc tcatgcccgc
     6661 cccagacccc ggcaatgcgg tacatgtcag gcaatttgga attggagtgc tggctgacat
     6721 agagtaccga gaagtcgttg gtgtaaaagg cataagtcag gcaagcgaaa gagaaggcag
     6781 tcaatagcca caatgcctga gccccagggc gggcgaatgc catccagtca gcacggtttt
     6841 gctggccacc cgcaaccccg ataaccccca ggcagaagga caccagcagg gccaggataa
     6901 gggcgaaaga cccgagttca ggaatcatgg aaggtgcgcc ttatttcagg gttttgatgg
     6961 tgttctgcgc ttgatcaacc gcatgtttgg cttctggcgg catgtagttt tcatcatgtt
     7021 ttgccagtac ttcggtggcg gtgaaatgac cactggcatc gagcttgccc tgtgccacaa
     7081 cacctttgcc ttccctgaac aagtccggca agatgccggt gtaagacacc ctgatctctt
     7141 tggccgtgtc ggtgacaaca aaattgacgg tcaggttgtc gcgattgacg ctgcccgact
     7201 tgaccagccc gccaactcga aaagtccggt ttcttggggc ctcgccggac tctatctggc
     7261 tgggggagaa gaagaatacc aggttgctct caaatgcgtt gaggacaaaa taggccgcca
     7321 gaccaatggc gaccaagcca ccagcgatga tggcggcacg tttgtgacga ggtttcatga
     7381 tgagtgaaga gttgcgtcag gttgtgactc aatttcgctg ataaggttgc gcaaaatctg
     7441 ccgccggtgc gacctcacct gccatatttc tgcggcaatc actgcagcag ttgccccaaa
     7501 gctgccccag acataaaagg cgtaaccgcc catgttgaaa aaatctgcca agctaagcca
     7561 ctgcatgttt cacccattcg gcatggtgtt cccgctcaag aatgatgttg cgcacccgca
     7621 gcaaggccat ggcaattgca tacatccaga aagccagcac catgatcaac atgccggcca
     7681 gcatggtgct ggccatggaa ctgcctttga gcgacaccga cgcaccctgg tgcaaggtgt
     7741 tccaccactt gaccgaaaag taaatgattg gcacgtttac cgcgcctacc agcgccagga
     7801 cggcaccggc cttgtctgcc cgccgcgggt cgtcaatgct ggcctgcagc gccatgaaac
     7861 ccagataaag aaacagcaaa atcagcgttg atgtcagccg ggcgtcccag acccaccaag
     7921 ccccccacat gggtttgccc cataaggcac cggtcagcaa cgacaaaaat gcaaacagcg
     7981 cgccggtggg ggccagggcc gaggccatca tgcccgacag gcgggtgttg aaggccagac
     8041 ccagaccagc ccatgcggcc atcaccaggt agatgaacat ggccatctgg gaggccggca
     8101 catgaacaaa aatgatgcga tagccttggc cttgttgtgc atcaatgggt gcgacaaaca
     8161 aggcgatcca caggcctacc agcgccagca gcgttgccaa agcagcaaac cagggccaca
     8221 tcttgccggc caaatggtag aagttttgcg gtgaggcgaa cttgaaccaa tgaatcacag
     8281 gggttttcat tccagggaaa tcctcaaggc agcagcggtg gcaaaaggtg aaaaaaacaa
     8341 cgaaagtacc agcagtgccg aaagcagcga caagtggcca ccaaagccga ctcctgccgc
     8401 gtcagcttcc accgcgccag caccaaaaat cagcactgga atgtacagcg gcaggatcaa
     8461 caaggaaagc aaaaccccgg caccgcgaac gcccaatgtc agggcggccc caatgctgcc
     8521 aatcaggcta aggctgggcg cgccaagcaa caaagtcagc atcaaaatgg ccagggcgcg
     8581 tgcatccagt ccaaattgca ggcccaatac cggtgccatc aacaccaacg gcagacccga
     8641 caagacaaaa tgggccaagg ccttcgccag taccagcagg cccagcgggg tcggcgacaa
     8701 caccatttgc tccagtgaac cgtcggcgta gtcggtggca aacatgcgtt gcaaagagag
     8761 catggcagcc agcagcgcac tgacccacaa cacccccggt gccatgcgca gcagcaggct
     8821 ggattcagct ccgacaccca gcgggaacaa gctggcaatc accatgaaaa acaccagagg
     8881 tgtcagcact tcgccctttt gccgcatggc caaagcgact tcgcgcttga gcacggcaag
     8941 catgacgtga atcaaaccga tggatttgac agtctgactc atggggcttt gagttccagc
     9001 atctgggccg gatgctggcc aatcggaaca cgctggtggc tggtcagcac cgccagccca
     9061 ccctgatcga gatggtgggc tatcaagtca gatagcagtt gaatgccgcc ttcgtccatt
     9121 gccacataag gttcatctag cacccacaaa cgcgccggac tcagcgctag ccgggacagc
     9181 gcaacgcgac gcttttgccc ctgtgacaag tgccgcacca attgttgcga ccgaccgcgc
     9241 atgccaaacc gcgccagcac ctcctggctc tgctggtgat caggggcgat gccaccgagc
     9301 gcagagg
//
LOCUS       DAOSLL010000113         9269 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7100_length_9269_cov_4.743325, whole genome shotgun sequence.
ACCESSION   DAOSLL010000113 DAOSLL010000000
VERSION     DAOSLL010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9269
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7100_length_9269_cov_4.743325"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="PLB25_06800"
     CDS             complement(<1..850)
                     /locus_tag="PLB25_06800"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HOE41323.1"
                     /translation="MSPIPPSMADRDGKIWMDGQMVDWRDAKIHVLTHTLHYGCGVFE
                     GVRAYNTAVGPAIFRLQEHTERLFNSAKILRMKIPFTLDEVMEAQKAVIRDNQLDSGY
                     LRPLSWIGDKKLGVSPRGNTIHLMVAAWPWGAYLGEEGMKRGIRVKISSYTRHHVNIT
                     MTQAKAVSNYTNSILANLEVTDDGYDEAMLLDANGFVSEGAGENVFIVKGGVVFTPDL
                     SAGALNGITRNTVLHICKDLGLEVVQKRITRDEVYISDEAFFTGTAAEVTPIRELDRI
                     ELGTGSR"
     gene            complement(1013..2350)
                     /locus_tag="PLB25_06805"
     CDS             complement(1013..2350)
                     /locus_tag="PLB25_06805"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HOE41324.1"
                     /translation="MDRRFFLSAGGILLAAASPLAWADDKKKTGSGSGSSTSSSTSTS
                     TAPTGSAIKSRVIVIGGGMAGATVAKYLRLWGDGIAVTLIDRNSSYVSNIMSSMVLTG
                     QRTLTSLTYSYSKLQSAYGVTVVKGEVSNVDPSKGQVQLADGTILTADRIVMAPGIEF
                     DDVPGLGSSDRMPHAWQAGPQTTLLQKQLSAMTAGGTVILTIPQKPYRCPPGPYERAC
                     LIGDWLKTYKKGSQLIVLDANSDYVAERDNFVAGFKLHNIKYVPNSAVQSVDPGTSST
                     GLMSIKTTDGVTHQANVVNMIPRQKAGGLVVKTGLANATEGRFAGVNQLTYASTVSGA
                     GKVHIIGDSSATDQPKAGHIANQEAKVCADAIVRSLAGLQPDPAPVTNSACYSTITMS
                     QASWLMAMYQYDPAAAKMALAAVPGVAASTGWNADNFEDMNTWFSALMADSFA"
     gene            complement(2375..2683)
                     /locus_tag="PLB25_06810"
     CDS             complement(2375..2683)
                     /locus_tag="PLB25_06810"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOE41325.1"
                     /translation="MRHFFRTAVGAAVCTLGLSLAAQAQTAPPAGRLLASNCFQCHGT
                     NGKGPGFDKLTGESSSEIYKDLKEFASGKEGSGIMAKHAMGYTDAQLQSLSNWLSTQR
                     "
     gene            complement(2775..4208)
                     /locus_tag="PLB25_06815"
     CDS             complement(2775..4208)
                     /locus_tag="PLB25_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE41326.1"
                     /translation="MTLLPRLKSLQARLLALLLMMMALVWLAAAVLTWIGTRHELDEL
                     LDGHLAQSAALLVAQQTIQDQDQDDNQKSDPPGRHDDDAQPLHKYAPRVAFQVFHEGQ
                     LTLRSANVGDTPMSTTLRGFETVQLPDQSRWRVFATQGNEGDVQVFVGERTESRHDIL
                     WAVMTGVLTPLLYSLPLLGLVGWLAVRQALAALRQLSRLLAKRQPQALDAVVLGDLPA
                     EIEPVVQSLNALFGRIGNMLSSERRFTADAAHELRTPIAAIRIQAQVALGAGGDIAER
                     DHALGLTIEGCDRAAHLVEQLLTLSRLEASSASAPAGLVNLSALAQRVAADLAPTALQ
                     NGQVLELEASVPGLIQVDDTLTCVLLRNLTDNALRYSPPGAKVCLSVTTEDNRVLLQV
                     DDSGPGLPDVDMARLGERFFRCMGTTQSGSGLGWSIVRRIAAVYQAEVKVCRSTLLGG
                     LCVSVIWPVHAAQPVSNGVIDGGRRHL"
     gene            complement(4221..4892)
                     /locus_tag="PLB25_06820"
     CDS             complement(4221..4892)
                     /locus_tag="PLB25_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOE41327.1"
                     /translation="MRLLLAEDDPMLGDGLRAGLRQQGFQVDWVRDGLAAEREIASGD
                     YQAAVLDLGLPQKDGLEVLQALRRKKITTPVLVLTARDTVPDRIKGLDLGADDYVIKP
                     VDLFELGARLRSLVRRAHGLVQDVLNCGNVQMNLAARQVLMDGQAVSLSTREFDLLHA
                     LMLNMGRVLSREQLEQQLYSWGYEVESNAIEVHIHHLRKKLRADLIQTLRGVGYMLLR
                     PKDPT"
     gene            complement(5561..7534)
                     /locus_tag="PLB25_06825"
     CDS             complement(5561..7534)
                     /locus_tag="PLB25_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE41328.1"
                     /translation="MALITLLDAQLAFGHVALLDHADFSLETQERVGLIGRNGAGKSS
                     MLKILGGLDKPDDGTLQVQGGTRIAYVAQEPQLDPDATVFVAVSAGVARARALIDEYS
                     QGHGDLDAMQSEIEALDGWNWEQRVSETLHRLHLDPDAVLATLSGGTKKRVALAQALV
                     TQPDVLLLDEPTNHLDLDSIEWLEGLLVDFKGSMITITHDRSFLDNVATRIVELDRGR
                     LLSYPGNFAAYVLQKEEQLAQEAVINARADKLLAAEEVWIRKGVEARRTRAQGRITRL
                     QVLRANRAARRDVIGSVNMDVASGVGSGKLVAELTHVSKSFGERRIVHDFSATILRGD
                     KIGLLGPNGAGKTTLLKLILGELKADPAPANSPPPESGHSPWGTVRQGANIEVAYFDQ
                     MRNALDLDATLEDFISPGSEWIEIGSQKKHVKSYLGDFLFSPARANSPVRSLSGGERN
                     RLLLARLFARPANVLVLDEPTNDLDIDTLELLEDLLQNFDGTVFLVSHDRTFLDNVVT
                     STIAFEGNAHWREYEGGVQDWLTQSRRAGEIAKNSAQKGAQPNKGVREQLSKEQQYKA
                     TTATLAEPVASLVPPAKARKLSFREQRELDGLPDLIARLEAEQKDIAGLLADGSLYAI
                     DNTRALKLASRSAQIDDELLGALERWELLGNAG"
     gene            complement(7768..>9269)
                     /locus_tag="PLB25_06830"
     CDS             complement(7768..>9269)
                     /locus_tag="PLB25_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOE41329.1"
                     /translation="LGVGDVSLIESAHEQWLALSGLGLDVVFKVLVPALTLAVLLSID
                     TLKTCVVIDAMTNSSHDSNRELVGQGLGNIASALIGGVPGAGTMGASVVNISSGGSTR
                     RSGLLAGGFSLLAFLLLAPLIAWVPVAALAAILIVVGARMIDRHSLKFFYSPATRLDF
                     MVILSVILVALFGNLIAASAVGVALAILLFIREQTRSSVIHSRVEGTQVFEKGTRFGK
                     EPGNVDPDHQSMVVFELQGSLFFGTANQLHQALEPETANHKYVILSMRRVQSLDVTAT
                     HVLEQIKDRLEENNAYLVFCDIPKGLPSGLKMKRFLKETGVVHGSNKAFAFRALDEAL
                     EWVETRRAESAQPDPVEAVPLALRDFPLLAGQPEAALAALEAVALPRSIKTGKKVFKA
                     GDADSELYFVRRGSVKITLPLHKKDNYHLATAGRGSLIGAMGFLDPEGHTGNAVALSD
                     TDVYALTRDGFNNLAEQHSALALVILLAVAGNLSNRLHITITELQALRG"
BASE COUNT         1795 a         2819 c         2755 g         1900 t
ORIGIN      
        1 cccggctgcc agtaccgagt tcgatccggt cgagttcgcg aatcggtgtc acttcagcgg
       61 cggtgccggt gaagaaggct tcgtcagaga tgtacacctc gtcacgggtg atgcgctttt
      121 gcaccacctc caggcccaaa tccttgcaga tgtgcagcac ggtgttgcgg gtaatgccgt
      181 tgagtgcacc agccgacagg tcaggcgtaa agaccacgcc gcccttgacg atgaagacgt
      241 tttctccggc accctcggag acaaaaccgt tggcatcaag cagcatcgcc tcgtcgtagc
      301 cgtcgtcggt gacctccagg ttggccagaa ttgaattggt gtagttgctg accgccttgg
      361 cctgagtcat ggtgatgttg acatggtgcc gggtgtagct tgaaatcttg acgcggatgc
      421 cgcgtttcat gccttcttcg cccaggtagg caccccaagg ccacgcggcc accatcaggt
      481 gaatggtgtt gccccggggg gatacgccga gctttttgtc gccaatccag gacagcgggc
      541 gcaggtagcc ggaatcgagc tggttgtcgc ggatgacagc tttctgtgcc tccatgactt
      601 catccagggt aaacggaatt ttcatgcgca ggattttggc gctgttaaag agtcgctcgg
      661 tgtgctcttg cagccggaaa atcgccgggc caaccgccgt gttgtaagca cgcacaccct
      721 caaaaacacc gcagccatag tgcaaggtat gggtcagcac atggattttg gcatcgcgcc
      781 agtccaccat ctggccgtcc atccagattt tgccgtcgcg gtcagccatg gaagggggta
      841 tggggctcat cggttgttcc ttaaaagtca gcgttgcgct gaaggtcaaa accggtattt
      901 tacgaggctg gcccgcctct gagggtcact gattgacctg cccggggcat gaaagtcatt
      961 tcatgtgaac ccgggccagt ctgggttgaa tgcgccgcag cgcgcaccgc cctcaggcga
     1021 agctgtctgc catcaaggcg ctgaaccagg tattcatgtc ttcaaagttg tcggcattcc
     1081 agccggtgga agcagcaaca ccaggcacgg cggccagtgc cattttggcc gcagccgggt
     1141 cgtactggta catcgccatc aaccaggatg cttgcgacat ggtgatggtg gagtagcagg
     1201 ccgagttggt gacgggtgcc gggtccggct gcaaaccagc cagtgaacgc acgatggcat
     1261 cggcgcacac tttggcttcc tggttggcga tgtgtccggc tttgggctgg tcggtggcgc
     1321 tggagtcacc aatgatgtgc actttgccag cgccgctgac cgtgctggca taggtgagct
     1381 ggttgacacc ggcaaaacgc ccttcggtgg cgttggccaa accggtcttg accaccaggc
     1441 caccggcctt ttggcgcgga atcatgttga ccacgttggc ctggtgcgtc acaccatcag
     1501 tcgtcttgat gctcatcaaa ccggtgcttg acgtgcccgg atcgacactt tgaacagccg
     1561 aatttggcac gtacttgatg ttgtgcagct tgaagccggc cacaaagttg tctctttctg
     1621 cgacgtagtc ggagtttgcg tcaagcacaa tcagttggga acccttcttg taggttttca
     1681 gccagtcgcc gatcaggcag gcacgctcgt aagggccggg cgggcaacgg tagggttttt
     1741 gcggaatggt caagatcacg gttccaccgg ctgtcatggc agacaattgc ttttgcagca
     1801 aggtggtttg tggaccggct tgccaggcgt gcggcatgcg gtcggagctg cccagaccgg
     1861 ggacatcgtc aaactcaatg ccgggtgcca tcacgatgcg gtcggcggtc aagatggtgc
     1921 cgtcagccaa ttggacttgt ccctttgacg ggtcaacatt ggacacctca cctttaacca
     1981 cggtcacccc gtaagccgac tgcaacttgc tgtagctata ggtcaggctg gtcaaagtgc
     2041 gctgcccggt caacaccatc gaactcatga tgttggaaac atagctgctg ttgcgatcaa
     2101 tcagcgtgac cgcaatgcca tcgccccaca ggcgcaaata tttggctact gtggcacccg
     2161 ccatgccacc gccaatcacg atcacccgtg atttgatcgc gctgccagtt ggtgcggtcg
     2221 aggtgctggt gctgctgctg gtactgctgc cgctgcccga accggtcttc tttttgtcat
     2281 cggcccaggc caatggtgag gctgctgcca gcaagatgcc gccggccgag agaaaaaatc
     2341 gtctatccat gatggaactc ctgaggtatt ggggttagcg ctgggttgac agccagtttg
     2401 agagcgactg caactgggca tcggtgtagc ccatggcatg tttggccatg atgccgctgc
     2461 cttctttgcc cgacgcaaac tctttcaggt ctttgtagat ttccgacgac gattcgccgg
     2521 tcagtttgtc aaaccccgga cctttgccgt tggtgccgtg gcactggaag caattggagg
     2581 ccagcaagcg tccggccggt ggtgcggtct gcgcttgcgc ggccagactc agccccagtg
     2641 tgcagacggc agcgccgacc gcagtgcgaa aaaaatgtct catggtgaac ctttctttga
     2701 gttaagtatc agtagtttcg gtaattacga tgtaagcgtc cgtagcaatt ggtaagcatt
     2761 tgttaccacg tgttttacag gtgtcggcga ccgccgtcga tgacaccgtt ggaaaccggt
     2821 tgtgccgcat gtactggcca aatcacgctg acacacaacc cgcccaacag ggtagagcgg
     2881 cacaccttca cctcggcctg gtataccgct gcgatgcgcc gcacaattga ccagcccagg
     2941 ccgctgcctg actgtgtggt gcccatgcac cggaaaaagc gttcgcccag acgcgccatg
     3001 tcaacatctg gtaaacccgg gccactgtca tcgacttgca gcagcacccg gttgtcttct
     3061 gtggtcaccg acaggcagac tttggcaccc ggcgggctgt aacgcagggc gttgtcggtc
     3121 aggttgcgca gcagcacgca ggtcagcgtg tcatcgacct ggatcaggcc gggcacagat
     3181 gcttcaagtt ccagcacctg cccgttttgt agcgccgtgg gggccaggtc ggcagcgacg
     3241 cgctgcgcca aggcagaaag attgacgagc ccggcaggcg cactggcgct gctggcttcg
     3301 agccttgaaa gggtcagcaa ttgctcaacc aggtgggcgg cgcggtcgca accttcgatc
     3361 gtcaagccca gcgcgtgatc acgctcggca atgtcgccgc cagcccccag cgccacctgg
     3421 gcctgtatgc gaatggcggc aatcggcgta cgcaactcat gggcggcatc agcggtaaag
     3481 cgccgctcgc tgctcagcat gttgcctatg cgcccaaaca aggcgttcag cgactgcacc
     3541 accggttcaa tctcagcggg gaggtcgccc aaaaccacgg catcgagtgc ctgcggctgg
     3601 cgctttgcca gcaaacggct caactgacgc agcgcggcca gagcctggcg caccgccagc
     3661 cagccaacca agcccagcag gggcaaggag taaagcaagg gggtcagcac ccccgtcatc
     3721 actgcccaca aaatgtcatg cctcgactcg gtccgttcgc cgacaaacac ctgcacatca
     3781 ccctcattgc cttgcgtggc aaacacccgc cagcgtgact gatctggcaa ctgcacagtc
     3841 tcaaagccgc gcagcgtagt tgacatgggg gtatcgccta cgttggctga gcgcagcgta
     3901 agctgtcctt catggaacac ctgaaacgcc acgcgcgggg catacttgtg caacggctgc
     3961 gcgtcatcgt catgccgccc gggcgggtcg cttttctggt tgtcgtcctg gtcctggtcc
     4021 tggatggttt gctgagccac cagcaatgcc gccgactgcg ccaggtggcc gtccagcaat
     4081 tcgtcgagtt cgtggcgggt gccaatccag gtcagcactg ccgctgccag ccagaccagc
     4141 gccatcatca tcaacagcag ggccagcaag cgtgcttgca gtgatttcag tcgcggcaac
     4201 aatgtcatgt gcactctgct tcaggtcggg tctttcgggc gcagcagcat gtagccaaca
     4261 ccgcgcaggg tctggatcag atcggccctg agctttttgc gcaggtggtg aatgtgcacc
     4321 tcgatggcat tgctttcaac ctcatagccc caactgtaga gctgctgctc aagctgctca
     4381 cgcgacaaga cccggcccat gttgagcatc agcgcatgca gcaagtcgaa ttcgcgggtg
     4441 gataagctga ctgcctgtcc gtccatcagt acctggcgtg ccgccagatt catttgcaca
     4501 ttgccgcagt tcagtacatc ttgtaccagg ccatgggcgc gccgcaccag cgaacgcagg
     4561 cgcgcaccga gctcaaacag gtcaactggc ttgatcacat agtcatcagc acccaaatcc
     4621 agccccttga tgcggtcggg caccgtgtca cgggccgtca gcaccagcac cggggtggtg
     4681 attttttttc gacgcagcgc ctgcagcacc tccagcccat ctttttgtgg cagacccagg
     4741 tcaagtaccg ccgcctggta gtcgccgctg gcgatctcac gctcggcggc caggccgtcg
     4801 cggacccagt ccacctgaaa accctgttgc cgcagtccgg cacgcaagcc gtcgccaagc
     4861 atcgggtcgt cttctgccag taatagtcgc atgaaattcc taggtgccgt gtgtgccggt
     4921 gattatggtg accagacacc cgctgtcgct gtgccatgtg gcaggagcca gacaaaggtg
     4981 tcatgcgggt catgcccgct cggtgaagcc tggcagcact gtagccgcga cggattaata
     5041 ctggcttaaa agacccgtca gcctaaagcc agatgcagca gttgcggccg gctttgggtt
     5101 gcctgtgcgg tatctctttg cggttgtgcg cgtgcgcagc ctctgaacct tctatccgag
     5161 ttgcctttgt ctgcaaatgt cgtggtctgg cggcgcgatc atggcagcgc cagcctgttg
     5221 gtcaaacgac cgggagatga caacgcagcg ggactgattg ccgtgccgca cagccgccca
     5281 tggcaggccg acttccatca cggcagtatc gacaaatctt ccacaaggga agaagcgagt
     5341 cagcggtgct tgacgccatt gcgcctccgg ttttgtacaa gcagtagcct cgcgggcatt
     5401 cggcaaaacc tcggcatttc cgctggctgg ccgtgacatg gcatgactcc atctggattg
     5461 aggcagtcat ttcgactcaa tgaaccagaa atggcatcac tggcgtcggt tctaactgct
     5521 cgatccatca gcccgacagc ctcctgcgcc agcaccaacc ttagccagca ttgcccagca
     5581 gttcccagcg ctccagtgcg cccagcagtt cgtcgtcaat ctgggcactg cggctggcca
     5641 gtttgagggc gcgggtgttg tcgatggcat acaggctgcc gtcggccagc aacccggcaa
     5701 tgtccttttg ctcggcctcc agcctggcga tcaggtcggg cagaccgtca agctcgcgct
     5761 gttccctgaa actgagcttg cgcgctttgg cgggcggcac caggcttgcc acgggctcgg
     5821 caagggttgc cgtagtggct ttatactgct gctcttttga tagctgctcg cgcacgccct
     5881 tattgggctg agcgcctttt tgagcactat ttttggcgat ttcgcccgcg cgtcggcttt
     5941 gcgtcagcca atcttgcacg ccaccctcgt actcacgcca atgggcattg ccctcaaagg
     6001 cgatggtgct ggtcaccacg ttgtcgagaa aagtgcggtc atggctgacc agaaacacgg
     6061 tgccgtcaaa attctgcagc aggtcttcaa gcagttcgag cgtatcaatg tcgaggtcat
     6121 tggttggctc gtcgagcacc agcacattgg ccggtcgggc aaacaagcgt gccagcaaca
     6181 gccggttgcg ctcgccgccg ctgagtgagc gaaccggcga gttggcgcgc gcgggcgaga
     6241 acaaaaagtc gcccagatag cttttcacgt gctttttctg gctgccaatc tcgatccact
     6301 cgctgcccgg actgatgaaa tcttccagcg tggcatcaag gtcaagtgcg ttgcgcatct
     6361 ggtcaaaata cgccacttcg atgttggcac cctggcgcac tgtgccccag gggctgtggc
     6421 ccgactcggg cggcggcgag ttcgctggtg ccgggtcggc cttgagttca cccagaatca
     6481 gcttgagcag cgtggttttg cccgcaccgt tgggcccaag caggccaatc ttgtcgccac
     6541 gcaaaatggt ggcagaaaag tcgtgcacga tgcggcgctc accaaagctc tttgacacgt
     6601 gggtgagttc agccaccagc ttgccactgc cgacaccgct ggccacgtcc atgttgacgc
     6661 tgccaatcac atcgcggcgg gcggcgcggt tggcacgcag cacctgcaag cgggtgatgc
     6721 ggccctgcgc acgggtgcgc cgcgcttcga cacccttgcg aatccacacc tcttcggcgg
     6781 ccaacagctt gtcggcacgc gcgttgatca ccgcctcttg cgccagttgt tcttcctttt
     6841 gcagcacata ggcggcaaag ttgccggggt agctcagcag ccggccacgg tctagttcga
     6901 cgatgcgggt tgccacgttg tccagaaagg agcggtcgtg ggtgatggtg atcatgctgc
     6961 ccttgaaatc gaccagcaag ccttcaagcc actcgatgct gtcgagatcg aggtggttgg
     7021 tcggctcatc gagcagcaac acgtcgggct gcgtcaccag cgcctgcgcc agcgccacgc
     7081 gctttttggt gccgcccgac agggtggcca gcacggcatc agggtcgagg tgcagccggt
     7141 gcagggtttc gctgacgcgc tgttcccagt tccagccgtc cagcgcctcg atctcgcttt
     7201 gcatcgcatc cagatcgccg tggccctggg aatattcatc aatcaaggct ctagcccttg
     7261 ccacacctgc gctgacagct acaaatacag tagcatctgg atcaagttgc ggctcttgcg
     7321 cgacataggc aatgcgggtg ccgccttgca cttgcagcgt gccatcgtcg ggcttgtcga
     7381 ggccgccgag gattttgagc atgctcgatt tgcccgcgcc gttgcggcca atcaggccca
     7441 cgcgctcctg ggtttccagc gaaaagtcag cgtggtcgag cagcgcaacg tgcccaaatg
     7501 cgagttgagc gtcaaggagt gtgataagtg ccatgtggag cggattatcc ccggcgttgc
     7561 cagccacgga catcgcggcc aaggcggaat tttgccgggc accaaagcgc tgtttggcag
     7621 cggcagggtt cgcagccgtc tgcgccgggc agcagcactg accgccttgg ccgccatgca
     7681 ggctgccgct gcaccaggca cacgggccag accggtcttg ccggtgcggt cgggttgcag
     7741 cagatgcgcc acaagtccgg caggttgtca gccgcgcaag gcctgcaatt cggtgatcgt
     7801 gatgtgcagc cggtttgaca aattgcccgc caccgcaagc aggatgacca gtgccaaggc
     7861 gctgtgctgt tcggccaggt tgttgaagcc gtcacgcgtc aaggcataca catcggtgtc
     7921 gcttagcgcg actgcattgc cggtatggcc ttcggggtcc aaaaagccca tcgccccgat
     7981 gaggctgccg cgccccgccg ttgccagatg gtagttgtct tttttgtgca gcggcagcgt
     8041 gatcttgacc gagccacggc gaacgaaata gagttcgctg tcggcatcac ctgccttgaa
     8101 tacctttttg ccagtcttga tgctgcgtgg cagcgccacg gcttcgagcg ccgccagtgc
     8161 cgcctcgggc tgcccggcca gcaagggaaa atcacgcagc gccagtggca ctgcctcgac
     8221 cgggtcaggc tgcgcacttt cggcccgccg ggtttcgacc cattccagcg cttcgtccag
     8281 cgcacgaaag gcaaaggcct tgttgctgcc gtgaacgaca ccggtttctt tcaagaatcg
     8341 cttcatcttg agcccgctcg gcaggccttt gggaatgtcg cagaacacca ggtaagcgtt
     8401 gttttcttcc agccggtctt tgatctgttc cagcacatgg gtggcggtga catcgagcga
     8461 ctggacccgg cgcatgctca agatgacata tttatgattg gcagtttctg gttcgagcgc
     8521 ctgatgcaac tggttggcgg tgccaaaaaa caggctgccc tgcagttcaa aaaccaccat
     8581 cgactggtgg tcagggtcca cattgccagg ctctttgcca aagcgggtgc ctttctcaaa
     8641 cacctgggtg ccttcaactc ggctgtggat caccgagctg cgggtttgct cgcggataaa
     8701 caacaaaatg gccagcgcca cgccgactgc cgacgcggca atcaggttgc caaacagtgc
     8761 caccagaatc accgacaaaa tcaccatgaa gtccagccgg gtggccggac tgtagaaaaa
     8821 cttcaggctg tggcgatcaa tcatgcgtgc gccaaccaca atcaaaatcg ctgccagcgc
     8881 cgctaccggc acccaggcaa tcagcggtgc cagcagcaaa aacgccagca gtgagaagcc
     8941 accggccagc agtccgctgc gccgggtact gccgccactg gagatattca ccaccgaggc
     9001 ccccatggtg ccggcacccg gcacgccgcc aatcagcgcc gaagcaatat tgcccagccc
     9061 ttgcccgacc agttcgcggt tggagtcgtg gctggaattg gtcatggcat caatcaccac
     9121 acaggtcttc aaggtgtcaa tcgacagcag caccgccaat gtcagcgcag gcaccagcac
     9181 cttgaacacc acatcaagcc ccagtccgct caaggccagc cattgttcat gggcgctttc
     9241 aatcaaactg acatcaccca cccccagcg
//
LOCUS       DAOSLL010000114         9261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7107_length_9261_cov_4.620465, whole genome shotgun sequence.
ACCESSION   DAOSLL010000114 DAOSLL010000000
VERSION     DAOSLL010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9261
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7107_length_9261_cov_4.620465"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..884)
                     /locus_tag="PLB25_06835"
     CDS             complement(240..884)
                     /locus_tag="PLB25_06835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOE41330.1"
                     /translation="MKKLNKLAMLIATAALATAAGAQTIDNWRSADGIVWKNGTNELC
                     WRNNFWTPATAAEGCDGAIVAPKVAPAPAPAPVAAPAPAPAPAPAPAPAPVVATKVTY
                     AADAFFDFDKAVLKPEGKAKLDDLVGKVKDINLEVIIGVGHTDSVGSDAYNQKLSVKR
                     SEAVKAYLVSKGIEKNRVYTEGKGEKQPVADNKTSEGRAKNRRVEIEVVGTRGK"
     gene            1131..3782
                     /gene="gyrA"
                     /locus_tag="PLB25_06840"
     CDS             1131..3782
                     /gene="gyrA"
                     /locus_tag="PLB25_06840"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HOE41331.1"
                     /translation="MTQFAKETLPTSLADEMRRSYLDYAMSVIVGRALPDARDGLKPV
                     HRRVLFAMHELNNDWNKAYKKSARIVGDVIGKYHPHGDQSVYDTIVRMAQDFSMRHML
                     VDGQGNFGSVDGDNAAAMRYTEIRLAKIAHELLADLDKETVDFGPNYDGSEKEPLVLP
                     TRLPNLLVNGSGGIAVGMATNIPPHNLSEVVDACLHLLRHPDATIDELMEIIPAPDFP
                     TAGIIYGIQGVKDGYRTGRGRVVMRAKCHFEDIDKGQRQSIIVDELPYQVNKKTLQER
                     MAELVHEKKIEGISHIQDESDKSGMRLVIELKRGEVPEVVLNNLYKQTQLQDTFGMNM
                     VALINGQPKLCNLKDLIEVFLNHRREVVTRRTVFELRKARERGHVLEGLAVALANIDE
                     FIRIIRESPTPPVARAELMARPWDSKLVREMLTRTRADGGVVNADDYRPNGLEREFGM
                     GSDGLYRLSETQAQEILQMRLQRLTGLEQDKIVAEYKEVMAEIDDLLDILARPERVTV
                     IISDELTAIKTEFGLTKIGARRSLVEHSSFDLSTEDLITPTDMVVTLSHSGYIKSQPL
                     SEYRAQKRGGRGKQATATKEDDWVDQLFIANTHDYILCFSNRGRMYWLKVWEVPQGSR
                     GSRGRPIVNMFPLQDSEKVTVVLPLTGEKRTFPADQYVFMGTSMGTVKKTALDEFSNP
                     RKAGIIAVDLDEGDFLIGAALTDGKHDVMLFSDGGKAVRFDENDVRPMGRQARGVRGM
                     MLEDGQTVIAMLVAEDESQSVLTATQNGFGKRTSITEYTRHGRGTKGMIAIQQSERNG
                     KVVAATLVHADDEIMLITDRGVLVRTRVSEIRELGRATQGVTLIGLDEGSSLSGLQRI
                     VENDAIPVVDDASADEEGSDGSTLPEA"
     gene            3894..4976
                     /gene="pheA"
                     /locus_tag="PLB25_06845"
     CDS             3894..4976
                     /gene="pheA"
                     /locus_tag="PLB25_06845"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HOE41332.1"
                     /translation="MAKTLAELRNLIDALDLELIALLNKRATLAHEVGEIKRQDGSAV
                     FRPDRETQVIANLQANNDGPLKPHSVALIWREIMSACRAIEAPLRMAYLGPAGTFSEQ
                     AAIQFFGGSVARVMCVSFDEVFRATAAGSAEFGVVPIENSSEGVVSRTLDLLLNSPLH
                     IIGETSFLVRHNLLRLVPSLDGIEVVYAHPQALAQCQDWLTTHLPDADRRAASSNAEG
                     ARLASTNPAWAAIASERAGSEFGLHVAFHAIQDDAFNRTRFAVICLPQTLATPLATGH
                     DCVSLIVSVANRPGAVHDLLVPLKNHGVSMTRFESRPARSGQWEYYFYIDLLGHPSQP
                     NVRAALDELRALCAFYKLLGTYPVAV"
     gene            4979..5848
                     /locus_tag="PLB25_06850"
     CDS             4979..5848
                     /locus_tag="PLB25_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HOE41333.1"
                     /translation="MFEQLGLIGCGLMGGSFALALKKAGLVKRVVGYSKSPSTTERAR
                     QLGVIDVEAPSALLAVSGADIVLVAVPVSASEATFKSIRHMVTPEMLIMDVGSTKGDV
                     VRAAERGLRDQIGSFVPAHPIAGKEMAGVEHAEADLYQQKQVILTPVEQTLTAQLRQA
                     IELWTALGCKVVQMSPESHDAAFAAVSHLPHLLSFALMNAIAGQAAGKDYLSLAGPGF
                     RDFSRIAASDPKVWRDIFMTNRDELLLQSRAFQNQLSAFEALMSSGDAQGLEDQIARA
                     SAARSQWRMNQLD"
     gene            6175..8184
                     /locus_tag="PLB25_06855"
     CDS             6175..8184
                     /locus_tag="PLB25_06855"
                     /EC_number="2.5.1.19"
                     /EC_number="2.7.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phosphoshikimate
                     1-carboxyvinyltransferase/cytidylate kinase"
                     /protein_id="HOE41334.1"
                     /translation="MFATKFLDLPALESIGGVITLPGSKSISNRVLLLAALCPGRTAV
                     RDLLDSDDTQVMLDALRALGCGVEQQGNTVVIEGMDGQPVRRQTRLFMGNAGTAIRPL
                     TAALAVIGGDHELRGVPRMHERPIGDLVDALRQLGCQIDYLEKPGYPPLRIGQPSLKL
                     DVPIRVRGDVSSQFLTALLMALPLVAEKDIVIEVVGELISRPYIEITLNLLARFGVTV
                     QRDGWQRFTIPAGSKLSSPGVVHVEADASSASYFIAAGAIAERARGQKGIKIQGLGAN
                     SIQGDIRFIEAAQMMGANIISGPNWLEVSRGEPGRGWPLRAIDLDCNHIPDAAMTLAV
                     MALYANGTTRLRNIASWRVKETDRITAIACELRKLGATVQEGSDFIHITPPATLADWQ
                     AASIHTYDDHRMAMCFSLVAFNPAGVPVRIEDPKCVAKTYPDYFEDLLSLVRTQTVNI
                     PVICVDGPTASGKGTLAAELARQLGYHLLDSGALYRITALAAVRAGLPLDTAGEAAIA
                     SLLQVLKIDFVQHRVLLDGADVTEAIRTEEVGMNASRISALALVRHALVGLQHNFRRL
                     PGLVADGRDMGTVIFPDARLKVFLTAGPRQRAQRRFKQLISKGNSTTLEGLQADLEAR
                     DERDQTRSVAPLKAASDAKLLDNSDLSVEESIDLVLNWWQGVQPF"
     gene            8316..>9261
                     /locus_tag="PLB25_06860"
     CDS             8316..>9261
                     /locus_tag="PLB25_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HOE41335.1"
                     /translation="MSESFAALFEESLQRTEMRPGEVITAEVVRIEHSFVVVNAGLKS
                     EAYVPLEEFKNDQGETEVQVGDFVSVAIGSIENGYGDTILSRDTAKRLASWMSLEKAL
                     ESGEFVTGTTSGKVKGGLTVLVNGIRAFLPGSLVDTRPTKDLSPYENKTLEFKVIKLD
                     RKRNNVVLSRRAVVEASMGEERSKLMNTLKEGAIVQGVVKNITEYGAFVDLGGIDGLL
                     HITDMAWRRVRHPSEVVTAGQEITAKILKFDTEKNRVSLGLKQMGDDPWMGVNRRYPQ
                     GTRMFGKITNIADYGAFVELEPGIEGLVHVSEMDWTNKN"
BASE COUNT         1990 a         2721 c         2696 g         1854 t
ORIGIN      
        1 caggcgttgc gctgcttcat cgtgtatttg cataactcac cttcacaagt ttcaaagcac
       61 cgggttgtca aacccgaaca tgaatggttc aacactgaac cataacgccg acacagccta
      121 gattttaggg cgtgctcttt ttcaatcaac cgtttcacga cagcaggtac gcaatctgca
      181 cgcaaaaaaa cccgcctagg cgggtttctt tgacaagcgt atcaggctat tgctggcaat
      241 tacttgccac gtgttccaac cacctcgatt tcaacgcggc gattcttggc gcgaccttca
      301 gaggtcttgt tgtcagcaac aggttgcttc tcgcccttgc cttcggtgta gacacggttc
      361 ttttcaatac ccttggacac cagataagcc ttgacagctt ccgagcgctt gaccgacagc
      421 ttctggttgt aagcatcaga gccaactgaa tcagtgtgac cgacaccaat gattacttcc
      481 aggttgatat ctttcacttt gccaaccagg tcgtcaagct tggctttacc ttcgggcttg
      541 agcactgcct tgtcaaagtc aaagaacgca tctgccgcgt aagttacctt ggtggcaaca
      601 acaggagcgg gagcgggagc gggagcgggt gccggtgccg gtgccggagc ggctaccggt
      661 gcaggtgcag gcgcaggcgc aaccttgggc gcgacgatgg caccgtcaca accttcagcg
      721 gcagtagcgg gtgtccagaa attgttacgc cagcaaagtt cattggtgcc gttcttccag
      781 acgatgccat cagcagaacg ccaattgtcg atcgtttgag cgccggctgc ggtagcgaga
      841 gctgcggtag cgatcaacat cgccagtttg ttcaattttt tcatgattct cctagaagaa
      901 agtgccgcag cggtgcggcg gtggtgaaac ggacgctcaa aatgcctatc acattgagcg
      961 ttacattgat tgtgccacat agattttcag ccaaaacgat accgccgatc aaaaccaacg
     1021 caaggggccg gtgccccatg acttttgttt caggcgttgt gaaggtacaa caaacccaca
     1081 gcataaaatc agcctaacaa cctgaacttg cttcgcctca agcctttccc atgacccagt
     1141 tcgccaaaga aaccctgcct accagcctgg cggatgaaat gcgccgtagc taccttgatt
     1201 acgcgatgag cgtgatcgtc gggcgggcgc tacctgatgc gcgtgacggc ttgaaaccgg
     1261 tgcatcggcg cgtactgttt gccatgcacg aattgaacaa cgactggaat aaggcgtata
     1321 aaaaatctgc gcggattgtc ggcgacgtga tcggtaaata ccacccgcac ggcgaccagt
     1381 cggtctatga caccattgtt cgcatggcgc aggatttctc aatgcgccac atgctggtcg
     1441 atggccaggg caatttcggc tcggtcgatg gcgacaacgc tgccgcgatg cgttacaccg
     1501 aaatccggct ggccaaaatt gcccacgagt tgctggctga tctggacaag gaaaccgtcg
     1561 atttcggccc caactacgac ggctctgaaa aagagccgct ggtgctgcca acccgcctgc
     1621 cgaatctgct ggtcaatggc tccggcggca ttgcagtcgg catggccacc aacatcccgc
     1681 cgcacaacct cagcgaagta gtcgatgcct gcttgcatct gctgcgccac cccgatgcca
     1741 cgattgacga gctgatggaa atcatcccgg caccggactt tccgactgcc ggcatcattt
     1801 acggcattca aggggtcaaa gatgggtacc gcaccggtcg cggccgggtg gtgatgcgtg
     1861 caaagtgcca ttttgaggac attgacaagg gacagcgcca gtccatcatt gttgatgagc
     1921 tgccctacca ggtgaataaa aagacattgc aagagcgcat ggccgagctg gtgcacgaga
     1981 agaagataga aggcatcagc cacatccagg atgaatccga caagtccggc atgcgcctgg
     2041 tgattgaact caagcgtggc gaagtgcccg aggtggtgct caacaacctg tacaagcaga
     2101 cccagttgca agacaccttt ggcatgaaca tggtggcgct gatcaatggt cagcccaagc
     2161 tgtgcaacct gaaggatttg atcgaggtct ttttgaacca ccgccgcgag gtggtgacac
     2221 ggcgcaccgt gttcgagtta cgcaaggcgc gcgaacgcgg ccatgtgctc gaaggcctgg
     2281 cggtggcgct ggccaatatt gacgaattca tccgcatcat ccgcgagtca ccaacgccac
     2341 cggtggcccg cgccgagctg atggccagac cctgggacag caagctggtg cgcgaaatgc
     2401 tgacccgcac ccgggccgat ggcggcgtgg tgaatgccga cgactaccgt ccaaacgggc
     2461 tggagcgtga atttggcatg ggttcagatg gcctgtaccg gctgtctgaa acccaagctc
     2521 aagagatctt gcagatgcgc ctgcagcgcc tgaccggcct ggagcaggac aagatcgttg
     2581 ccgagtacaa agaggtcatg gccgaaattg acgacctgct cgatattctg gcacgccctg
     2641 agcgcgtcac ggtcatcatc agcgacgagc tcaccgccat caagaccgag ttcggcctga
     2701 ccaagattgg cgcgcgacgc agcctggttg agcactcgtc gttcgatctt tccaccgaag
     2761 acctgatcac cccgaccgac atggtggtca ccctgagcca tagcggttac atcaagagtc
     2821 agccgctctc cgaataccgg gcgcaaaagc gcggcgggcg cggcaaacag gccaccgcca
     2881 ccaaggaaga cgactgggtt gaccagctct ttattgccaa cacccacgat tacatcctgt
     2941 gtttttccaa tcggggccgc atgtactggc taaaggtctg ggaagtgccg caaggttcac
     3001 gcggctcgcg cggtcggccc atcgtcaaca tgttcccgct gcaagacagc gaaaaggtca
     3061 ccgtggtgtt gcccctgacg ggcgaaaaac gcacttttcc ggcagatcag tatgtcttca
     3121 tgggcaccag catgggcacc gtcaagaaga ccgcactcga tgaattcagc aacccgcgca
     3181 aggccggcat cattgccgtt gatctggacg aaggcgactt tttgatcggt gcggcgctga
     3241 ccgacggcaa gcatgacgtg atgcttttca gtgatggcgg caaggcggtg cgctttgacg
     3301 agaacgatgt gcgcccgatg ggtcgccagg cccgtggcgt gcgcggcatg atgcttgaag
     3361 atggccagac cgtgattgcc atgctggtcg ccgaggacga gtcgcaaagc gtgctgacgg
     3421 ccacccagaa cggctttggc aagcgcacca gcatcaccga atacacccgc cacggtcgtg
     3481 gcaccaaggg catgatcgcc atccagcagt ctgagcgcaa cggcaaagta gtggccgcca
     3541 cgctggtaca tgcggacgac gaaatcatgt tgatcaccga ccggggcgtg ctggtacgca
     3601 ccagggtcag cgaaatccgc gagctcggtc gcgccacaca aggcgtgacg ttgatcgggc
     3661 tggacgaagg ctcaagtttg agcggcttgc agcgtatcgt cgaaaacgac gcgattccgg
     3721 ttgttgacga cgcgtcggcc gacgaggaag gcagcgacgg cagcaccctg ccagaagctt
     3781 gattactatc tattcaatag ctactaacgc aatcaagata agggctagag cctgatttgt
     3841 tgactatttc atgatcgacc aacgttctgg atcaactcct atttcatccc gccatggcca
     3901 agacccttgc tgaactgcgc aacctgatcg atgcgcttga tctggaactg attgccctgc
     3961 tgaacaaacg tgccaccttg gcgcatgaag tcggcgaaat caagcgacag gacggctcgg
     4021 cggtgttccg gcccgaccgt gaaacccagg tcatcgccaa cctgcaggcc aacaacgacg
     4081 gccccctcaa gccgcacagc gtagccctga tctggcgcga aatcatgtct gcctgccgcg
     4141 ccatcgaagc ccccttgcgg atggcctacc tcgggccggc tggaaccttc agcgaacagg
     4201 cggcaatcca gttttttggc ggcagcgtgg cacgcgtgat gtgcgtcagc tttgacgaag
     4261 tgtttcgtgc cacggccgca ggcagcgccg agttcggcgt ggtacccatc gagaactcct
     4321 cggagggcgt ggtatcacgc acacttgact tgctgctcaa ttcaccgctg cacatcattg
     4381 gcgaaaccag tttcctggtg cgccacaatt tgctgcgcct ggttcccagt ctggacggca
     4441 tcgaagtggt ttatgcccac ccgcaagcct tggcacagtg tcaggactgg ttgaccacac
     4501 atctgcctga cgcagaccgt cgcgccgcct ccagcaatgc cgagggtgcc cgtttggcca
     4561 gcaccaaccc ggcctgggcg gccatcgcca gtgagcgtgc gggtagcgaa tttggtctgc
     4621 acgtggcctt ccacgcaatt caggacgatg ccttcaaccg cacccgcttt gccgtgatct
     4681 gcctgccgca aacgctggcc acgccgctgg ccaccggcca cgattgcgtc agcctgatcg
     4741 tctcggtagc caaccgtccc ggcgcggtgc atgacctgct ggtgccgctg aaaaaccatg
     4801 gtgtctcgat gacccgtttt gagtcgcgcc cggcgcgctc gggccagtgg gagtattact
     4861 tctacattga tctgctcggc cacccgtcgc agccaaatgt gcgtgcggca cttgacgaat
     4921 tgcgcgcctt gtgcgcgttt tacaagctgc tgggtaccta cccggtggcg gtctgagcat
     4981 gtttgagcaa ctcggtctga ttggctgcgg cctgatgggc ggctcatttg cgctggcgct
     5041 gaaaaaggct ggcctggtca agcgtgtggt gggctacagc aaatcaccct ccaccaccga
     5101 acgtgcccgc caattgggcg tgatcgatgt cgaggcaccc tcggcgctgc tggcggtctc
     5161 gggtgccgac atcgtgctgg tggcagtgcc ggtgtcggcc tcggaggcga ctttcaagtc
     5221 gatcagacac atggtcacgc cagaaatgct gatcatggat gtcggctcca ccaaaggcga
     5281 tgtggtgcga gcggccgagc ggggcttgcg cgatcagatc ggctcatttg tcccggcaca
     5341 cccgattgcg ggcaaggaaa tggccggtgt cgagcacgcc gaagctgatc tttaccagca
     5401 aaagcaagtc atcctgactc cggttgagca gaccctcacg gcccagctca ggcaggcaat
     5461 cgagctctgg acggcactgg gctgcaaggt ggtgcagatg tcgcccgagt cgcatgatgc
     5521 ggcgtttgca gcggtcagcc acctgccgca cctgttgtct tttgcgctga tgaacgcgat
     5581 tgccggacaa gccgcaggca aggactattt gtcattggcc ggtcccgggt ttcgggattt
     5641 cagccgcatt gcggcaagcg acccgaaagt ctggcgtgac atcttcatga ccaaccgcga
     5701 cgagttgctg ctgcaaagcc gggctttcca gaatcagttg tcagcctttg aagccctgat
     5761 gagcagtggc gatgcgcagg gcctggaaga ccagatcgcc cgcgccagcg ccgcccgcag
     5821 tcagtggcgc atgaaccagc tcgactaagg acatggtcac caacaacttc cgcatttggc
     5881 ccgtcgggtc tgggcgcact gtgccgttac aaatcgccag tgtggctcgc cagacatcgc
     5941 gctttgcgcc tggcaatgca gcctgaaccc aacggccaaa tgcgtaactt gttttcgacg
     6001 ctgtcccaag ggcacatggc gcaatggcag gcacatttgg cgcgtcatct tcgggcgcat
     6061 tgcgtgcatg caaattgccg gtttggctaa gccaactggc acgctctgcg cctggcgttg
     6121 cagcccaaac ccgacacccc aatgcacaac ctgtgaggta accagccctg acccatgttt
     6181 gccaccaaat ttctcgactt gccggcgctt gagtcgatcg gcggtgtgat caccctgcca
     6241 gggtcaaaaa gcatctccaa ccgggtgctg ctgctggcag ccttgtgccc agggcgcacc
     6301 gccgtgcgtg acctgcttga ctcagacgac acccaggtca tgctcgatgc cctgcgcgca
     6361 ctgggttgcg gtgttgagca acagggcaac accgtggtga tcgagggcat ggacggccaa
     6421 ccggtcagac ggcaaacccg cttgttcatg ggcaacgctg gcaccgcaat acgaccgctg
     6481 acggcggcgc tggccgtgat cgggggcgac catgaactgc gcggcgtgcc tcgcatgcac
     6541 gagcgcccga ttggcgacct ggtagacgcg ctgcgtcagt tgggttgcca gatcgattac
     6601 ctggaaaagc caggttatcc gccgctgcgc attggtcagc caagcctgaa actcgatgtg
     6661 ccgattcggg tgcggggtga tgtgtcgagt cagtttctta ccgctctgct gatggcgctg
     6721 ccgctggtgg ctgaaaagga cattgtcatc gaggtcgtcg gtgaactgat ctcgcggccc
     6781 tacatcgaga tcacgctaaa cctgctggcg cgctttggtg tcaccgtgca acgcgatggc
     6841 tggcagcgtt tcaccattcc tgcgggcagc aaattgagct cccctggcgt ggttcatgtt
     6901 gaagcagacg cttcatctgc tagttatttc atagctgctg gcgcaatagc tgaaagggct
     6961 agaggccaaa aaggcataaa aattcaaggc ctgggcgcga attcgattca gggcgacatc
     7021 cgcttcattg aagccgcgca aatgatgggt gcaaatatca tcagcggccc caactggctg
     7081 gaagtgtcgc gcggcgagcc gggtcgcggc tggcccttga gagccattga tctggattgc
     7141 aaccacattc cggatgccgc aatgacgctg gccgtgatgg ccttgtatgc aaacggcacc
     7201 acccgcctgc gcaatattgc cagttggcgc gtcaaggaga ccgaccgcat cacagccatc
     7261 gcctgcgagt tgcgcaaact cggtgcgacg gtgcaagaag gcagcgactt catccacatc
     7321 acgccaccgg ccaccctggc cgattggcag gccgcaagca tccacaccta cgacgatcac
     7381 cgcatggcga tgtgcttttc attggtcgcc ttcaacccgg cgggtgttcc ggtgcgcatt
     7441 gaagacccaa aatgcgtcgc caagacctac ccggactatt ttgaagacct gctgtcgctg
     7501 gttcgcaccc aaaccgtcaa catcccggtc atttgcgtgg acggaccaac cgcgtcgggc
     7561 aaaggtaccc tggctgcaga attggcccgc caactgggct accacctgct ggattcgggt
     7621 gcactctacc gcatcaccgc actggcggcc gttcgtgccg gtttgcccct ggacactgct
     7681 ggcgaggcag ccattgccag tctgttacaa gtgctcaaga ttgattttgt acagcaccgg
     7741 gtgctgcttg acggtgctga cgtgaccgag gcgatccgta ccgaggaagt cgggatgaac
     7801 gcttcgcgca tctccgcgct ggcgctggtg cgtcatgctt tggtcggcct gcagcacaac
     7861 tttcgtcgcc tgccgggact ggtcgctgac gggcgcgaca tgggcacagt gatctttcca
     7921 gatgcccggc tcaaggtctt tttgaccgca ggcccccgac agcgtgcaca aagacgcttc
     7981 aaacagttga tttcaaaggg aaattcaact acactcgagg gtctgcaggc tgatttggaa
     8041 gcgcgtgacg agagggatca aacccgtagc gtcgcgccac tcaaagcggc atcggatgcc
     8101 aaactgctgg acaactcgga tttatcggtt gaagaatcaa ttgatctggt gttgaactgg
     8161 tggcaaggcg tgcagccctt ctgatcactc ggaaggacca aaaccgaaag gcttaccccc
     8221 ttgcaacggc cctcaaccgc cctatcgtgc tgcactggca cattgcggat gaggttcaaa
     8281 aaacttaacc ccgcggttaa cacaccgcat tgaaaatgtc tgaatctttt gccgccctgt
     8341 ttgaagagtc cctgcaacgc acagaaatgc gccccggtga agtcatcacc gccgaggtgg
     8401 tccgtattga acacagcttt gtggtggtca acgctggcct gaaatccgaa gcctatgtgc
     8461 cgctggaaga attcaagaat gatcagggcg aaaccgaagt gcaggtcggt gacttcgtct
     8521 cggtagccat cggctcgatc gaaaacggct acggcgacac catcctgtca cgcgacactg
     8581 ccaagcgttt ggcatcatgg atgagcctgg aaaaagccct ggagtccggc gaatttgtca
     8641 ctggcaccac cagcggcaag gtcaagggtg gtctgaccgt tctggttaac ggtatccgcg
     8701 cctttctgcc cggctcactg gtcgacaccc gtccgaccaa agacttgtct ccgtacgaaa
     8761 acaagacgct tgaattcaag gtcatcaaac tcgaccgcaa acgcaacaac gtggtgctga
     8821 gtcgccgcgc cgtggtcgaa gccagcatgg gtgaagagcg cagcaagctg atgaatacgc
     8881 tcaaggaagg cgcgattgtc caaggtgtgg tcaagaacat cactgagtac ggtgcttttg
     8941 tcgatctggg cggcattgac ggcttgcttc acatcaccga catggcttgg cgccgtgtcc
     9001 gtcacccgtc tgaagtggtg accgctggcc aggaaatcac cgccaagatc ctgaaattcg
     9061 ataccgagaa aaaccgtgtc tcattgggac tgaagcaaat gggcgacgac ccttggatgg
     9121 gcgtgaaccg tcgctacccg caaggcacgc gcatgtttgg caagatcaca aacatcgccg
     9181 actacggtgc atttgtcgag ctggaacccg gcatcgaagg cctggtgcac gtgtccgaga
     9241 tggattggac caacaagaac g
//
LOCUS       DAOSLL010000115         9253 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7114_length_9253_cov_4.087954, whole genome shotgun sequence.
ACCESSION   DAOSLL010000115 DAOSLL010000000
VERSION     DAOSLL010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9253
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7114_length_9253_cov_4.087954"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="PLB25_06865"
     CDS             complement(<1..582)
                     /locus_tag="PLB25_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOE41336.1"
                     /translation="MLKQRVITALLLMLVLLPALLYPDPAVFYLIAMVFIAAAGWEWA
                     RLNGLDRLASLMSGGICVLACCWLWWAGLLGQALPRVWLVVGGTWVLSSAWVLKAGVS
                     AWSQHPRSLRMLLGLLALCLTWLAVAQARLIGINFLLSVLALVWVADIGAYFSGRALG
                     GRFSRSKLAASISPGKSWEGVWGGMLGVVVLALV"
     gene            complement(592..1320)
                     /gene="uppS"
                     /locus_tag="PLB25_06870"
     CDS             complement(592..1320)
                     /gene="uppS"
                     /locus_tag="PLB25_06870"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HOE41337.1"
                     /translation="MTGAQVPHHVAIVMDGNGRWANKRLLPRIAGHKQGVDSLKRCAK
                     ACDARGIRVLTVFAFSSENWQRPVDEVSGLMDLFAFALGREVPQLKADGVQLRFVGER
                     QGLSAKVLRGIENAEAATAANTRLVLNVCFNYGGRWDIVQAAARLAQQGLAVTEASLN
                     DAMALAHVPDPDLVIRTGGEQRISNFLLWQSAYSEFFFTDLLWPDFDEAALDLAITSF
                     SQRQRRFGKTSDQLDGRPQSARVA"
     gene            complement(1328..1888)
                     /gene="frr"
                     /locus_tag="PLB25_06875"
     CDS             complement(1328..1888)
                     /gene="frr"
                     /locus_tag="PLB25_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HOE41338.1"
                     /translation="MPITDVKVNAEAKMDQSIAAFQHNLTKIRTGRANPALLDTVHVD
                     YYGALVPISQVANVSLIDSRTVSVQPWEKGMGAKIEKAIRDSDLGLNPSSMGDLIRVP
                     MPAMSEERRKELTKLVRSEGEGAKIAVRNLRRDANEAIKKLVKDKLASEDDQKRCESD
                     IQKVTDRHVVAIDQLVIAKEHDIMAV"
     gene            complement(1943..2665)
                     /gene="pyrH"
                     /locus_tag="PLB25_06880"
     CDS             complement(1943..2665)
                     /gene="pyrH"
                     /locus_tag="PLB25_06880"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HOE41339.1"
                     /translation="MSDANPAYKRILLKLSGEALMGDDSFGINRGTIVRMVNEIVEVT
                     RLGVEVAVVIGGGNIFRGVAGGSVGMDRATADYMGMLATVMNALALGDTMNKAGLTAR
                     VMSAIAIEQVVEPYVRPKALQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEIGAEIV
                     LKATKVDGVYTADPKKDPSATRYSKISFDDAMSQNLGIMDATAFALCRDQKLPVKVFS
                     IFKHGALKRVVLGEDEGTLVYA"
     gene            complement(2716..3639)
                     /gene="tsf"
                     /locus_tag="PLB25_06885"
     CDS             complement(2716..3639)
                     /gene="tsf"
                     /locus_tag="PLB25_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HOE41340.1"
                     /translation="MAAITASMVAELRGKTDAPMMECKRALTEAEGDMARAEEILRVK
                     LGSKAGKAASRITAEGVVAYSMVAGVGALLEVNCETDFVTKNDSFLALANAAAALIAK
                     HNPADIAALGALAHSQDGFGPTLEDARKGLIGKIGENMSFRRFKRFAAGHKLASYLHG
                     TRIGVVVEFDGDDIAAKDIAMHVAAMKPVALSSADVPAELVARERSVATAKAAEDAAV
                     ATAAGKPVQSAEIVAKRIEGGVQKYLKEVSLLNQSFVKNDKQTVEQMLKATGTTVKSF
                     TLYVVGEGIEKKVDDFAAEVAAQVAAAKQPS"
     gene            complement(3732..4487)
                     /gene="rpsB"
                     /locus_tag="PLB25_06890"
     CDS             complement(3732..4487)
                     /gene="rpsB"
                     /locus_tag="PLB25_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HOE41341.1"
                     /translation="MAVTMREMLEAGVHFGHQTRFWNPKMAPFIFGHRNKIHIINLEK
                     SLPMFQDAAKFVRQISAKRGTVLMVGTKRQARETVATEALRAGVPYVDQRWLGGMLTN
                     FKTVKTSIKRLKDMKVQQEAGLDAMSKKEQLMFARELEKLEKDIGGIQDMTVLPDAIF
                     VIDVGYHKIAIAEAKKLGIPLIAVVDTNHSPEGIDYVIPGNDDSSKAVTLYARGIADA
                     IIEGRANAMDDVVKAVVASESADEFVEVSEAVA"
     gene            4823..5620
                     /locus_tag="PLB25_06895"
     CDS             4823..5620
                     /locus_tag="PLB25_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOE41342.1"
                     /translation="MPAFPKSVQKIIELTRDVNCTPRDLVQVIEKDPVVTVKILKVVN
                     SAYYGLPKQITSISHAVVYLGFNTIKNLALSIAAIGMLSKHNEAGFDVDQYLLHSLST
                     AGIAKLLALRLDDADPMDCFIAGLLHDFGKVVFAQFLPHEFKLALAASQADGSSLHEA
                     LRQTVGADHAVVGAMLAEKWRFAPDLVDTIAHHYEADQKDSALFASVYTANQISKHLN
                     FGFGGNPCVYPLPPRVVKRMGGSLEEIIASSGDMNAAFEDAKLFARI"
     gene            5631..6518
                     /locus_tag="PLB25_06900"
     CDS             5631..6518
                     /locus_tag="PLB25_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE41343.1"
                     /translation="MKVKFWGVRGSIASPGPATVRYGGNTTCIEVRTDNNELIILDAG
                     TGIFPLSQSLLAELPVTANVLITHSHWDHIQGLPFFIPNFIPGNTLRLHGAFDPVSGK
                     GIEQVMAVQLQYSYFPVREAEMKARIEYVTLKPGESIQIGSAKITPYMMNHPVIDFGY
                     RIEANGKSVFFTGDHEPPSNIYMPDDPEFAEYQLFVDEKSNDILRAMAGVHMLIADCS
                     YTRAEYPAKKGWGHGTFDSCIHYARQAGAKLLVCTHHEPTRSDDALEAAFAQVLQEHP
                     RLPGDPQYRLAREGESFEF"
     gene            6522..7979
                     /locus_tag="PLB25_06905"
     CDS             6522..7979
                     /locus_tag="PLB25_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HOE41344.1"
                     /translation="MQKIVTGIGYALYGVLPTREIEAAAQVLLPPNTLMQRAGLAVAQ
                     LALALAPHAQRLWIACGPGNNGGDGLEAAVQLLRLGKSSTVTWLGNTESMPPDTVVAH
                     QRAIAAGVHFATEPDSDFDLCVDALLGIGTQSREPRGRMADWIGRMNAASAPVLSVDI
                     PSGLHADTGAAMQLHVKATHTLSLLTLKPGLFTAQGRDATGTVWLDSLGIHQNAAICP
                     PVSARLLGRPLQTTRAHSSHKGSFGDVAVVGGAPGMTGAALLAASAALHAGAGRVFVA
                     LLGISPLQVDASRPELMFRAASDIDFSQLTVVCGCGGGDAIVALLPRVIQTASAAVID
                     ADALNAIAADDVLQTALTRRCQSSLGTVLTPHPLEAARLLACSVAEVQRDRIAAALAL
                     VAKFGCTVVLKGSGTLIASPGQIPMINPTGNARLATAGTGDVLAGMVGASLAAGVPPF
                     EAACHAVYWHGQLADQWPTNRPFTAGLLASCPASF"
     gene            complement(8037..8888)
                     /locus_tag="PLB25_06910"
     CDS             complement(8037..8888)
                     /locus_tag="PLB25_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HOE41345.1"
                     /translation="MNLSLNVPSPLEYFASLVHTDADFALLEAAISLAQDEYPDINVG
                     QVLGEVDQLLARLLRRLPADAGPLQKLRSLNQFVYRDLNFAGNFNNYADPDNSYVHVV
                     LRTRLAIPISLAVVWLELAQGIGLKARGVSFPGHFMVKVNLSEGQVVIDPLTGQSLGR
                     EELAERLQPYRGRVSQSDEQELPLGLFLQAAPERDIIARMLRNLKEIHTAEEDWPRLL
                     AVLDRLVILLPQVWAEYRDRGLAHAAAGHAGHALEDLETYLANAGVVTDRSAMSARAA
                     ELRQALH"
     gene            complement(8901..>9253)
                     /locus_tag="PLB25_06915"
     CDS             complement(8901..>9253)
                     /locus_tag="PLB25_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HOE41346.1"
                     /translation="QLLNIVLVPIFAHAALTLSIGVGAMVNAAWLLVGLIRRGSYRPE
                     PGWAVFMLQVLAACVLMAVFLMWANGHFGWTASGFQGFKRIWLIALILSAAAAIYFLA
                     IWATGLKLRQLVRR"
BASE COUNT         2040 a         2754 c         2474 g         1985 t
ORIGIN      
        1 gaccagcgcc agaacgacca cgcccagcat gcctccccaa acgccctccc agctcttgcc
       61 cgggctgatg gatgcagcaa gtttgctgcg tgagaatcgc ccacccaagg cgcggcccga
      121 aaaataagcc ccaatgtctg cgacccatac cagcgcgagg accgagagca aaaagttgat
      181 gccgatgagt cgcgcctgcg ccaccgccaa ccaggtcaaa cacagtgcca acagacccaa
      241 cagcattcgc aaagaccttg gatgctggga ccaggccgaa acacccgctt tgagcaccca
      301 ggcactcgac agcacccagg taccacccac caccagccag acccgaggca gtgcctggcc
      361 caacaagcca gcccaccaaa gccagcagca tgccagtacg caaatgcctc ctgacatcaa
      421 cgatgccagg cggtccagac cattcaggcg cgcccattcc cagcccgcag cggcgatgaa
      481 aaccatggcg atcaggtaaa aaacggccgg gtctgggtag agcaaggctg gcagcaacac
      541 cagcatcaac agcagcgcag tgatcacccg ttgtttgagc atgatcagac tttatgccac
      601 acgggctgac tgcggccgac catcgagctg atcagatgtc ttgccaaaac gccgctgacg
      661 ttgggaaaat gacgtgatcg ccaggtccag cgcagcttcg tcaaaatcag gccataacaa
      721 gtcagtgaag aaaaactcgg agtacgctga ctgccacagg agaaaattgc tgatgcgctg
      781 ttcgccaccg gtccgaatga ccaaatcggg gtcaggaaca tgcgccagtg ccatggcatc
      841 gttcaaactt gcttcggtca cagccaggcc ctgctgtgcc agacgtgccg ctgcctgcac
      901 aatatcccag cgaccaccat agttgaaaca aacattcagc accagtcgcg tgttggcggc
      961 ggtggcggct tcggcatttt cgatcccccg cagcaccttg gcagacaagc cttgtcgttc
     1021 gccgacaaag cgcaactgga caccgtcggc cttgagctgt ggaacttcac gacccagcgc
     1081 aaaagcaaac aaatccatca gacccgacac ctcgtccacc gggcgctgcc agttctcgga
     1141 cgagaaagca aataccgtca gaacccggat gccacgtgcg tcacaggctt tggcgcagcg
     1201 cttcagggaa tccacgccct gcttgtgacc tgctatgcgc ggcagcaacc gcttgttggc
     1261 ccatcgccca ttgccgtcca tgacaatggc cacatgatgc ggtacctgag cacccgtcat
     1321 gaccgcgtca gaccgccatg atgtcgtgtt ccttggcaat gaccaattga tcaattgcca
     1381 caacgtgccg gtcggtgacc ttctgaatgt ccgattcaca gcgtttttga tcgtcttcgg
     1441 aggccaattt gtctttgacc agttttttga ttgcttcgtt ggcatctcgg cgaaggttgc
     1501 gcacggcaat cttggcacct tcaccttcgg atcgcaccag tttggtcaat tccttccgac
     1561 gctcttccga catggctggc atggggacac gaatcagatc gcccatcgag gagggattca
     1621 aacccaggtc gctgtcacgg atagcctttt caatctttgc tcccatgccc ttttcccaag
     1681 gctgaacact gacggtgcgc gaatcaatca gcgacacgtt cgccacttgg ctgatcggca
     1741 ccagcgcgcc gtagtaatcg acatgcaccg tatccagcag ggcagggttg gctcgaccgg
     1801 tacgaatctt ggtcaaattg tgctgaaagg cagcaatgga ctggtccatt tttgcttcgg
     1861 cattcacttt tacatcagta attggcatga tttaatcctt actccttgtt cagtcatcac
     1921 ccaggcaccg attttggcac gctcaagcgt aaaccagtgt gccctcatct tcacccagaa
     1981 caaccctctt gagcgcccca tgcttgaaga tggagaagac cttgacgggc agtttttgat
     2041 cccggcacaa ggcaaaggcg gtggcatcca tgatgcccag attttgcgac atggcatcgt
     2101 caaatgaaat tttgctgtaa cgcgttgcgc tcggatcttt cttggggtca gccgtgtata
     2161 caccgtccac cttggtggct ttcaacacaa tttcagcgcc aatctctgca ccacgcagcg
     2221 ctgccgcggt gtcggtggta aaaaacggat tgccggtacc agcggcaaag atcaccactt
     2281 tgccttcttc gaggtattgc agtgctttgg gtcgaacgta gggctcaacc acctgctcga
     2341 tggctatggc cgacatcacg cgtgcggtca accctgcctt gttcatggtg tcgccaagtg
     2401 ccagggcatt catcacggtg gccagcatgc ccatgtagtc agcggtggcc cggtccatgc
     2461 cgaccgagcc cccagctact cccctgaata tgttgccacc gccaatcacc accgccactt
     2521 ctacaccgag tcgggtcacc tcgacaattt cgttcaccat gcgaacaatc gtgccacggt
     2581 taatgccaaa gctgtcatcg cccatcagcg cctcacctga caacttgagc agaattcgct
     2641 tgtaggctgg attggcatcg gacatgaaat tctcctggcg tgatgaattg acgggctcga
     2701 atcacctgaa acagatcagg atggctgttt ggccgcagca acctgagccg ccacttcagc
     2761 ggcaaaatcg tccaccttct tctcgatgcc ctcgcccacc acatacaggg tgaacgactt
     2821 tactgtggtg ccggttgcct tgagcatctg ctcgaccgtt tgtttgtcat ttttgacaaa
     2881 ggactggttc agcaaggaca cttctttcag gtacttctgc acgccaccct caatgcgctt
     2941 ggcgacgatt tcggccgact gcaccggttt gccagcggcg gtggcgaccg ccgcgtcttc
     3001 ggcggccttg gcggttgcca ctgaccgttc ccgagccacc agctcagcag gcacatcagc
     3061 gctggacaat gccaccggtt tcatcgcagc cacatgcatg gcaatgtcct tggccgcaat
     3121 atcgtcacca tcgaattcga ccaccacgcc aatacgggtg ccatgcaggt aagaggccag
     3181 tttgtgacct gccgcaaaac gtttgaagcg gcgaaagctc atgttttcgc caatcttgcc
     3241 aatcaggcct ttgcgagcgt cttcgagtgt cgggccaaaa ccatcctggc tgtgcgcaag
     3301 cgcacccaaa gcggcaatat cagccgggtt gtgcttggcg atcaacgccg cagccgcatt
     3361 ggccaaggcc aaaaaactgt catttttggt tacaaagtca gtttcgcaat tgacttccag
     3421 caatgcgccc acaccagcaa ccatgctgta tgccacgaca ccttcagcgg tgatgcgact
     3481 ggcggccttg ccagccttgc tgccgagctt gacacgtaag atttcctctg cccgggccat
     3541 atcgccctca gcttcagtca gggcgcgctt gcactccatc atcggcgcgt cagtcttgcc
     3601 acgcagttca gccaccatgc ttgcggtaat tgcagccatt ctttttctcc ggatttaatt
     3661 cagttggtta gatatcaaat taaaaaaaaa gggcattaaa gccccctctt ttttttgggg
     3721 tcgctgcaaa ctcaggcaac agcctcgctt acttctacaa actcgtcagc gctctcggat
     3781 gcgacaactg ccttgacgac atcatccatt gcgttggcac ggccttcaat gatggcatca
     3841 gcaataccac gggcgtataa agtgacggcc tttgaagagt catcattgcc tggaatcacg
     3901 tagtcaatac cctctggaga gtggttggta tcgaccactg cgatcaaggg aatgccaagc
     3961 tttttcgcct cagcaatcgc aattttgtga tagccaacat caatcacaaa aattgcatca
     4021 ggtaacacgg tcatatcctg gatgccgcca atgtcttttt ccagcttctc aagctctcgg
     4081 gcaaacatga gctgttcctt tttactcatc gcatccaagc ctgcttcctg ctggactttc
     4141 atgtctttga gtcgcttgat agaagtcttg acggttttga aattcgtcaa catgccacct
     4201 aaccagcgtt gatccacata cggaacacca gcgcgcaacg cttcagtggc aacggtctcc
     4261 cgcgcttggc gcttggtgcc taccatgaga acggtgccgc gcttggccga aatctggcgc
     4321 acgaacttgg ccgcgtcctg aaacattgga agcgattttt ccaggttgat gatgtgaatt
     4381 ttgttgcgat gaccaaaaat gaaaggggcc atcttgggat tccagaagcg ggtctggtga
     4441 ccaaagtgga caccggcttc cagcatttcg cgcatggtaa ctgccatata ggaaactcca
     4501 aaggttatgt ctaaaatcca gtttgttttg cgacaggaaa caaggcagag ccgaattgat
     4561 gccgcaacac cttgagaaaa ctggtttgcg aatttgcctt gcctgttgtc aaaatttaac
     4621 aacaacgcaa agccaaacga gtatagcatt gtgacttttg cgggggctta acgaggtttt
     4681 ccccatactg atccttgaac cttgattaaa agttggtgtt tgcgagcgca atggcgtatc
     4741 taccctaatg gcaaatgatt gatcgacaat gcaagcaatg tccccggaac tttctcaaaa
     4801 gctcgtgagt gccgttgacg gaatgcctgc gttcccgaaa agtgttcaaa aaatcattga
     4861 gttgacgcgg gacgtgaatt gcacccccag agatctggtg caagtgatcg aaaaagaccc
     4921 ggttgtcacg gtcaagatcc tcaaagtcgt caactcagcc tattacggtc tgcccaagca
     4981 gatcacatcc atcagccatg ccgtggtgta tctgggcttc aacaccatca agaatctggc
     5041 gttgagcatt gcagcgatcg gcatgctgtc caaacacaac gaggccggct ttgatgttga
     5101 tcagtacctg ctgcactcgt tgtcaactgc cggcatcgcc aagttgctgg cattgcgtct
     5161 tgacgatgcc gacccgatgg attgtttcat tgccggcctg ttgcacgatt ttggcaaggt
     5221 tgtttttgcc cagtttttgc cccatgaatt caaattggcg ctcgccgcaa gtcaggccga
     5281 tggttcatcc ctgcatgaag cgctgcgcca gacagttggt gctgaccatg ctgtggtggg
     5341 agccatgctg gcggaaaaat ggcgctttgc acctgacttg gttgacacca ttgcccacca
     5401 ctatgaagcc gaccaaaaag acagcgcctt gtttgccagc gtttacactg ccaaccagat
     5461 cagcaaacac ttgaattttg gctttggcgg caacccttgc gtctatccat tgcccccgcg
     5521 cgtcgtcaaa agaatgggtg ggtcgctgga agaaatcatc gcatcctctg gcgacatgaa
     5581 tgctgcgttt gaggatgcca aattgtttgc ccgcatttag cagacccatc atgaaagtca
     5641 agttttgggg agttcgtggt tccattgcat cgccaggtcc tgccaccgtg cgctatggcg
     5701 gcaacaccac ctgcattgag gtgcgtactg acaacaacga actgatcatc cttgacgcgg
     5761 gcaccggtat ttttccgttg tcccagtccc tgctggctga attgccagta accgccaatg
     5821 tgttgatcac gcactcgcat tgggaccaca ttcagggttt accgttcttc atacccaact
     5881 tcattccagg caacacattg cgactgcacg gtgcttttga cccggtttcc ggcaagggca
     5941 ttgagcaggt catggccgtt cagcttcagt acagctactt tccggttcgc gaagctgaga
     6001 tgaaggcacg catcgagtac gttaccctga agccgggcga aagcattcag atcgggtctg
     6061 ccaaaatcac gccttacatg atgaatcacc cggtgattga ctttggctac cgcatcgaag
     6121 ccaatggcaa atcggtgttt tttaccggcg accacgaacc tccgagcaat atttacatgc
     6181 cagacgaccc ggagtttgcc gaataccagt tgtttgtgga cgagaaatcg aacgacatct
     6241 tgcgcgccat ggctggggtg cacatgctga ttgccgactg ctcgtacacc cgtgccgagt
     6301 atccggccaa aaagggctgg ggacatggca ccttcgactc ctgcatccat tacgcccggc
     6361 aagcgggtgc caagttattg gtgtgtaccc atcacgagcc aacacgcagc gacgatgcgc
     6421 tggaagcggc ttttgcgcag gtgttgcaag aacatccgcg cctgccgggt gacccgcagt
     6481 accgtttggc ccgcgaaggc gaaagtttcg agttctgagc ggtgcaaaaa attgttacgg
     6541 gcattggcta cgcgctgtac ggcgtgctgc cgacgcggga gatcgaggca gccgcccagg
     6601 tcctcttgcc acccaacacg ctgatgcagc gcgctggcct ggcggtggca caactggcgc
     6661 tggcgctggc accccatgcc cagcgcctgt ggatcgcctg cggcccaggc aacaatggcg
     6721 gtgacggtct ggaagcggca gtgcaactgc tgcgtctggg aaagtcgtcc accgtcacct
     6781 ggcttggcaa caccgaaagc atgccacctg acacggtggt ggcgcaccag cgggccattg
     6841 ccgcaggtgt ccacttcgcg accgaacccg acagcgactt cgacctgtgc gtcgatgccc
     6901 tgctgggtat cggcacgcag tcgcgtgagc cccgggggcg catggccgac tggatcgggc
     6961 gcatgaatgc cgccagcgcc ccagttttgt cggttgatat tccgagcggg ctgcatgccg
     7021 atacgggtgc tgccatgcaa ctgcacgtca aggcaaccca cacgctaagt ttgctgaccc
     7081 tcaaacccgg gctcttcacc gcccaagggc gcgacgcaac tggcacggtc tggctcgaca
     7141 gcctgggcat ccatcagaac gctgccatat gcccgccggt cagcgccaga ctactgggca
     7201 ggccgctgca gacaaccaga gcgcacagtt cccacaaagg aagctttggt gacgtggccg
     7261 tggtcggcgg cgcacccggc atgacgggtg ctgcgctact ggcagcctcg gcggccttgc
     7321 atgccggggc tgggcgggtt tttgttgctt tgctcggcat cagcccgttg caagttgatg
     7381 catcgcggcc cgagttgatg tttcgcgccg ccagtgacat cgatttctca caattgaccg
     7441 tggtctgcgg ttgcggtggt ggtgatgcga ttgtggccct gctgcccagg gttattcaaa
     7501 ccgcgtctgc cgctgtgatt gacgctgatg cactcaacgc catcgctgcc gatgatgtgt
     7561 tgcaaacagc cctgaccagg cgctgccaga gctcgcttgg cacggtgttg acacctcacc
     7621 cgctggaggc agctcgactg ctggcttgca gcgtggcaga ggtgcagcgc gaccgaatcg
     7681 cggcagcact cgccctggtc gcgaaattcg gctgcacggt ggtgctcaaa ggctccggca
     7741 cgcttatcgc gtcgccaggg caaataccga tgatcaaccc aactggcaat gcccggctgg
     7801 ccacggccgg taccggcgat gtcctggctg gcatggtcgg tgccagtctg gctgctggtg
     7861 tgccgccatt tgaagctgct tgccatgccg tctattggca cgggcaactg gcagaccagt
     7921 ggccgaccaa ccgtccgttc accgcagggt tgttggcgag ttgtccagca agcttttagt
     7981 gtgctatata aaaaatagct tgtggcgcaa tcaggataag ggctggaggc acattttcaa
     8041 tgtaaagcct ggcgcagctc agcggcgcgt gcggacatcg ccgagcggtc ggtaacgaca
     8101 cccgcgtttg ccaggtaagt ttccagatct tccagcgcat ggccggcatg acccgctgcg
     8161 gcatgggcca aaccccgatc acggtattcg gcccagactt gtggcaacaa aatgacgagc
     8221 cggtccagca ccgccagcaa gcgcggccag tcttcctcgg cggtgtgaat ctccttcaga
     8281 ttgcgcaaca tgcgggcgat gatgtcgcgt tccggtgccg cctgcaaaaa caggcccaat
     8341 ggcaattctt gctcatcaga ctggctgacc ctgccccgat agggctgcag tcgctcagcc
     8401 agttcttcgc gcccgagtga ctggccggtc aaggggtcaa tcaccacctg gccttcgctc
     8461 agattgacct tgaccatgaa gtgccccgga aaagacactc cgcgcgcctt cagtccaatg
     8521 ccctgggcca gttccagcca gactaccgcc aaagaaatgg gaatcgccaa cctggtgcgc
     8581 agcaccacgt ggacatagct gttgtcaggg tcggcgtaat tgttgaagtt gccggcaaag
     8641 ttcagatccc ggtaaacaaa ctggttcagc gagcgcaact tctgcagcgg cccggcatcg
     8701 gccggtaatc ggcgcagcaa gcgggccagc aactggtcca cttcgcccag gacctgcccg
     8761 acgttgatgt ctgggtactc gtcttgtgcc agactgatgg ccgcttccag cagcgcgaaa
     8821 tcggcatcgg tatgcaccag agaggcaaaa tactccagcg ggcttggaac attcagtgat
     8881 aggttcatgc ttgtcattgt ctaccgtcgc acaagttgac gcaatttcag cccagttgcc
     8941 caaattgcta gaaaatatat agctgctgcc gcagacagta taagggctat aagccatatt
     9001 ctcttgaaac cctggaaacc cgacgcggtc cagccgaagt gcccgttggc ccacatcaga
     9061 aagaccgcca tcaacacgca ggctgccagc acctgcaaca tgaacactgc ccagccgggt
     9121 tcgggccggt aactgccgcg ccggatcagt ccaaccagca gccaggcggc gttgaccatg
     9181 gcaccgacgc caatcgacag cgtcagcgcg gcatgcgcaa aaattggcac cagcacaatg
     9241 ttgagcaatt ggg
//
LOCUS       DAOSLL010000116         9244 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7122_length_9244_cov_4.667211, whole genome shotgun sequence.
ACCESSION   DAOSLL010000116 DAOSLL010000000
VERSION     DAOSLL010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9244
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7122_length_9244_cov_4.667211"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2378)
                     /gene="glnE"
                     /locus_tag="PLB25_06920"
     CDS             complement(<1..2378)
                     /gene="glnE"
                     /locus_tag="PLB25_06920"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HOE41347.1"
                     /translation="MTRPSLALHSRFAQRVRRRFDSQLTLLPAGLPTPAHLQGVFSAL
                     QGVGHDTGSALRIIRALVLERLLCLDCDAGAALEDVTRVMTDLAEFALNRALDEVLAK
                     LDLVHGAPQTQQGRRAELWVVGMGKLGARELNVSSDIDLIYVYDEDGDTAGDALGRGC
                     LSNHEYFAKVVRAIYALVGDSTEYGFVFRVDLALRPNGNSGPVALSLDALEEYLHVQG
                     REWERFAWLKSRVVAPHDSIVSGSSQALRKVVLPFVFRRYLDYSVFDSLRLLHHQIRD
                     HAAKRSAGRPERANDVKLSRGGIREIEFIVQLLQVVRGGQFPELRTRPTLNALVRLTK
                     AGLMLPDTARLLAQAYVFLRQLEHRIQYLDDQQTHVLPTQDADLAWIAATMGFDSVQR
                     LLNQLDAQRELVAQEFDKLLGGAEPECKGCHVGKQAASYPAMDELLPQLGKPFRQRLE
                     SWREHPRVLALREEARARLMRLLSRTAQWVRDGRVTEAAAVGLVDWMEPLLRRESYLA
                     LLLERPNVHERLLRLLGEARWPARYLLLHPGVIDELAASDMLRERFDAAGFEAELDYR
                     RTALRNAGEDDDEALLNLLRRAHHAEVFRTLARDIEGALTVEQVADDLSALADAVLRT
                     TTRWCWSRLKKAHRADPQFGIIAYGKLGGKELGYGSDLDIVFVFDDDDERAPEVYANL
                     VRKLINWLTVKTGEGDLYEIDTALRPNGNAGLLITSFTAYANYQQQRGSNTAWTWEHQ
                     AITRARCVLGDASLHARFEAVRKAVITAARNADDLRAEISAMRDRVAHAHPPR"
     gene            2410..6471
                     /locus_tag="PLB25_06925"
     CDS             2410..6471
                     /locus_tag="PLB25_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HOE41348.1"
                     /translation="MLSISSKLLKTWSGLTRWLLWATVLAWLTFGVFWATMHWLIVPR
                     IGEFRPLLEAQASQALGVTVRVGAVSARSSGLTPSFELTDVALFDSMGRVALSLPRVL
                     VALSPRSLWRLGFEQLYIDQPELDIRRTADGRITVAGLDFSSPAGTSTVALDWFFSQV
                     EFAIHDGTVRWTDEQRAAEPVQLQQVDLLVRNLGRHHDMRLDATPPDVWGARFNVQGR
                     FLQPLLARRNGQWRDWEGQLFAAFEHVDLRELRRYADPGFDLSQGRGALRAWVDVSRG
                     QITGATADVALSEVALTLAPDLQPMALQQLQGRISARVLSNGFEFATRSLMFDSPQGL
                     HWPGGNVRVLSLNAQGKTPARGEVQADRLDLAALTQIAHRLPLDAGLRNALIRYAPKG
                     QVDTLNASWQGTATAPVTYSAKGRLSQLELAAVDGAPGVRGLNLDFDLDQSAGRATLS
                     MTNGSVDVPGIFQEGLISIDQMSANAKWQTVQDHIAVQLTGLKFANADTQGEAQIKWQ
                     TSDPKKSPSKSRFPGILDLQAKLSRAEGRRVHRYLPLVIDQRARDYVREAVLDGNASN
                     VRFVVKGDIYDIPAVDARRGEFRISADVKAARLAYVPPGLQEPNELPWPALNDLSGEL
                     VIDRLQLMVKGARARLGDNVAVQVTQADATIADLLNAEVAVSADFRGPAPVVLQMVNG
                     SPLAELTARALTRTVVSGNADYKLKLLLPVADLAKSSVQGSISMSGNDLQISPDTPKL
                     TRTRGVLHFTQRGFTLAGVQTRMLGGDARLEGGLLLSGSDNSHPSAIRAAGLATAEGL
                     RQATELGLVARLARQISGSAAYNASFGLRRGVPELLVTSNLQGMAASLPSPLTKNAQS
                     LLPLRYQTASPVAPLSARRTPAPVQDRLTLTLGSLVSLTYERDVSGAEPRVLRGAIGI
                     GADTLDAVMLPEQGINANIHLPQFNVDAWADLLAQVTAAPLGTPDASKHPELAYLPSN
                     LAVRADVLTFGTRQFNRLVLGAGRDGLLWRANVDSAELNGYLEYRQPDAANANSAGAV
                     YGRLTRLIITPSIANDVEALLDTESASVPALDLLVDDFELRGKRLGRLEVEAVNRLGS
                     GPAGVNEWRLNKLNLSMPEATLTANGNWARLNAQNGGAPGASNRPERHRTVMNFKLDI
                     ADGGKLLNRFGMKDVVRQASGKIEGQLAWIGPPFSVDYPTLGGAVTVNVLSGQFLKAD
                     PGLAKLLGVLSLQALPRRLVLDFRDVFSDGFAFDFLRGNVAVEKGLARTNNLQMKGIN
                     AAVLMEGVADIDRETQDLKVVVVPELNAGTAALIATVINPAIGLGSFLAQLFLSKPLI
                     ESATQEFHVNGSWADPKVTKVKHITSTSKEKPQ"
     gene            6468..7301
                     /locus_tag="PLB25_06930"
     CDS             6468..7301
                     /locus_tag="PLB25_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOE41349.1"
                     /translation="MKVAVIQMVSGADLADNLRQAAELLSQAASQGAELVLLPEYFCQ
                     MGQRDADKLSICESLGSGPIQQFLVDAAKDLGLWLAAGTLPLAVDQIGGQSHSERVFN
                     STLVFSPDGSRVARYDKIHLFRFDNGTEHYDESVVLAAGTQPTSFDLPSRDGHIWRIG
                     LSICYDLRFAELYRHYASLGAHLLLVPSAFTYTTGQHHWDVLLRARAIENLAFVAAAA
                     QGGQHDNGRQTWGHSMLVDPWGLVLAQRPRDAGVVVAELDFGLLESCRSRLPALQHRV
                     L"
     gene            7307..8458
                     /locus_tag="PLB25_06935"
     CDS             7307..8458
                     /locus_tag="PLB25_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HOE41350.1"
                     /translation="MPLFFGAGLVALALWLTSCGNVPLHVPQTPDAARPTPPVPMPDA
                     ALPRTVLLRNKSQWVAVPWPDLPGFEDDALFEAWNAFIKSCERPAPVFAPLCPEIRRL
                     SIGTDAQQREWLLQRLQPYRVEPLQAEASTGLLTAYFEPEFEARRQAGAGFSFALHQA
                     PAGLTAGRPWFTRQEMETLPAAQAALRGREIIYLADPVQALIMQIQGSGRVRVTEPDG
                     SQRRLRLAYAANNGRPYQSIGRWLLDQGELRDASWPGIRAWLMQNPQRTAELLWRNPR
                     VVFFKEEPLNDFDAAFGPRGAQGVPLTPGRSIAVDPDSIPYGTPVWLASVGPSLSLQR
                     LVLAQDTGSAITGALRADYFVGWGAQAGELAGRLKQPLRLWVLWPKQQR"
     gene            8507..>9244
                     /locus_tag="PLB25_06940"
     CDS             8507..>9244
                     /locus_tag="PLB25_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HOE41351.1"
                     /translation="MNKSTMRDIDERTNLAGNSMFELLLFRLGEAPGTDRRELFGINV
                     FKVREILVMPEITAMVNSPPSVMGVANIRGQMISVINLPQIVGTNPTKGLGILLVTEF
                     ARTTQAFAVEEVNEIVRLEWKHVLSAEVRGGGLVTSIARLDGAAENTRLAQVLDVEQI
                     LRDVFPEQQQVELEPGNQLHVPAGTVILAADDSAVARMMIEQGLKGMEVPYIMTKSGQ
                     EAWDRLKAMAAEAESQGKTIRDKIALVL"
BASE COUNT         1760 a         2778 c         2887 g         1819 t
ORIGIN      
        1 cggggcgggt gggcatgcgc cacgcggtca cgcatggcgc tgatctcggc gcgcaagtca
       61 tcggcattgc gtgcagccgt aatgaccgcc ttgcgtaccg cctcgaagcg ggcgtgcagc
      121 gaagcatcgc ctagcacaca gcgggcgcgt gtgatggcct ggtgttccca ggtccaggcg
      181 gtgttggagc cgcgctgctg ctggtagttg gcataagcgg taaaactggt gatcagcaaa
      241 ccggcgttgc cgttggggcg caaggcggtg tcgatctcat acagatcacc ttcgccggtc
      301 ttgacggtca gccagttgat gagcttgcgg accaggttgg cgtagacctc gggggcgcgt
      361 tcgtcatcgt catcgaacac aaacacaatg tctagatcac tgccgtagcc cagttccttg
      421 ccccccagct tgccgtaggc aatgatgcca aactggggat ctgctcggtg cgctttcttg
      481 agccgactcc agcaccagcg ggtggtggtg cgcagcactg catcggccag tgcactgagg
      541 tcgtccgcca cctgctcgac cgtgagcgcg ccctcaatgt cgcgtgccag ggtgcgaaac
      601 acctcggcat ggtgcgcccg gcgcagcaaa ttgagcagtg cttcgtcgtc gtcctcgcca
      661 gcgtttctga gggcagtgcg gcggtagtcc agttcagcct caaaacccgc cgcatcgaag
      721 cgctcgcgca gcatgtccga ggcagccagt tcatcaatga cacctgggtg caacaacaaa
      781 tagcgggctg gccaacgtgc ctcgccgagc aggcgaagca ggcgttcatg cacgttgggg
      841 cgctccagca acagcgccag ataactttca cggcgcagca agggctccat ccagtccacc
      901 aggccaaccg ctgccgcctc agtcacccga ccatcgcgta cccattgagc ggtgcgcgac
      961 aacagccgca tcaaacgggc ccgggcctct tcgcgcaagg ccagcaccct ggggtgctcg
     1021 cgccaggatt ccagacgctg gcgaaacggc ttacccaact ggggcagcaa ctcgtccatg
     1081 gcggggtagc tggccgcttg cttgccgaca tggcagcctt tgcactcggg ttctgcacca
     1141 ccgagcaact tgtcaaactc ttgcgccacc agttcgcgct gcgcatcaag ctggttcagc
     1201 agccgctgaa ccgaatcaaa tcccatggtg gcagcaatcc acgccagatc ggcatcttgg
     1261 gtcggcaaaa catgggtttg ctgatcgtca aggtactgga tgcggtgctc aagttggcgc
     1321 aaaaacacat aggcctgcgc cagcaagcgt gccgtatcgg gcaacatcaa gccggccttt
     1381 gtcaggcgca ccagggcgtt cagggtgggg cgggtgcgca attcgggaaa ctggccacca
     1441 cgcaccacct gcagcagttg cacgatgaat tcaatttcac ggataccccc acgcgacagc
     1501 ttcacatcgt tggcccgctc cggtcggcca gcgctgcgct tggctgcatg gtcgcgaatc
     1561 tggtggtgca gcaagcgcag ggaatcaaac acgctgtagt ccagataacg cctgaacaca
     1621 aacggcagca ccaccttgcg cagcgcctgg ctggaaccgc tgacgatact gtcatgcggg
     1681 gccaccaccc ggcttttgag ccaggcaaag cgctcccatt cgcggccctg cacgtgcagg
     1741 tattcttcca gcgcgtccag cgacagggcc accggccccg agttgccatt ggggcgcaag
     1801 gccagatcga cccgaaagac aaagccgtac tcagtgctgt cacccaccag cgcataaatg
     1861 gcgcgcacca ccttggcaaa gtactcatgg ttgctcagac aaccacgccc gagcgcatca
     1921 cctgcagtgt cgccatcttc gtcatagaca tatatcaggt caatatcact cgacacgttg
     1981 agctcacggg caccgagctt gcccattccc accacccaca gctcggcccg cctgccctgt
     2041 tgggtttggg gtgcgccgtg caccaggtca agtttcgcca gcacttcgtc aagtgcccgg
     2101 ttcagggcaa attcggccag atcggtcatg acccgggtga cgtcctccag cgccgccccg
     2161 gcatcacagt ccaggcacag caaacgctcc agcaccagcg cccggatgat gcgcagtgca
     2221 ctgccagtgt catgaccaac gccttgcagc gccgaaaaaa cgccttgcaa atgcgcaggc
     2281 gttggcaagc ctgccggcaa tagggtcaat tggctgtcaa agcggcgacg aacgcgttgt
     2341 gcaaagcggg agtggagtgc cagggagggg cgggtcataa ttctggtctg cttattctat
     2401 tgaaacgatg tgttgtccat ctcatccaaa ctgctgaaaa cctggtctgg tctgacccgc
     2461 tggctgttgt gggcaacggt actggcctgg ctgacgtttg gcgtgttctg ggccaccatg
     2521 cactggttga ttgtgccgcg aatcggcgaa tttcgcccgc tgcttgaagc gcaagccagc
     2581 caggcgctgg gggtgacggt acgggtcggt gccgtttccg ccaggtccag tggcctgaca
     2641 ccttcgtttg aactgaccga tgtggccttg tttgacagca tgggcagggt cgctctgagt
     2701 ctgccccggg tgctggtggc actgtctcca cgctccttgt ggcggcttgg ctttgagcag
     2761 ctttacattg atcagcccga gcttgacatc aggcgcaccg ccgacggtcg catcaccgtc
     2821 gccggcctgg acttttccag cccggcgggt accagcacgg tagcgctgga ctggtttttt
     2881 tcgcaggtcg aatttgccat ccacgacggc acagtgcgct ggaccgacga gcagcgggca
     2941 gccgaaccag tgcagttgca gcaggttgac ttgctggttc gcaacctggg acgccatcat
     3001 gacatgcggc ttgatgccac gccgccagac gtgtggggcg cgcgtttcaa tgtgcaggga
     3061 cggtttttgc aacccttgct ggcgcgccgc aacgggcagt ggcgcgactg ggaaggtcag
     3121 ttgtttgcag cctttgagca cgttgacctg cgtgaactgc gccgttacgc cgatccgggc
     3181 tttgatctaa gccagggcag gggtgcgctg cgggcctggg tggatgtcag ccggggccag
     3241 atcaccggtg ccacggccga tgtggcgctt tccgaggtag ccctgacgct ggccccagac
     3301 ctgcaaccga tggcgttgca gcagttgcag ggacgcatca gcgcacgggt gctttccaac
     3361 ggttttgagt ttgccacccg ctcactgatg tttgacagtc cgcaggggct gcactggccg
     3421 ggtggcaatg tacgggtgct cagcctgaat gcgcagggca agacaccggc gcgcggcgaa
     3481 gtccaggcgg accggctcga tctggcagcg ttgacgcaga tcgcccatcg cctgccgctt
     3541 gatgcgggtt tgcgcaatgc cctgattcgt tatgcgccca aaggtcaggt ggacacgctc
     3601 aacgccagtt ggcagggcac ggccaccgcg ccagtaacgt acagcgccaa gggccgtttg
     3661 agccaacttg aattggcggc ggttgatggt gcacccggtg tgcgcggcct gaacctggat
     3721 tttgaccttg accaaagtgc cgggcgtgcc acgctgtcca tgaccaacgg cagtgtcgat
     3781 gtgccaggca tctttcagga agggctgatc agcatcgacc agatgtcggc caatgccaag
     3841 tggcagactg tgcaggatca catcgccgtg cagttgaccg gcctcaaatt tgccaatgcc
     3901 gacactcagg gcgaagcgca gatcaagtgg cagaccagcg acccgaaaaa gtcgccgtca
     3961 aagagccgtt ttccaggcat attggacttg caggccaagc tcagtcgcgc cgagggtcgg
     4021 cgggtgcatc gctatctgcc gctggtgatt gatcaaaggg cacgcgacta tgtgcgtgaa
     4081 gccgtgcttg acggcaatgc cagcaatgta cgttttgtcg tcaaaggcga catttacgac
     4141 atacccgcag tggatgcgcg ccgcggcgag ttcaggatca gtgcggatgt caaggcggcc
     4201 cggttggcct acgtgccacc cggtttgcaa gagcccaatg aactgccttg gcccgccttg
     4261 aacgatctca gtggtgaact ggtcatagat cgcctgcaac tgatggtcaa aggtgcccgt
     4321 gcccgtctgg gcgacaacgt ggcggtgcag gtcacgcagg ctgatgccac gattgccgac
     4381 ctgctgaacg cagaagtggc tgtcagtgcc gatttcaggg gcccggcacc ggttgttttg
     4441 cagatggtca acggttcacc gctggccgaa ctgaccgcac gcgcgctgac ccgcacggtg
     4501 gtgagcggca atgccgacta caagctcaag ctgttgcttc cagtggctga tcttgccaag
     4561 tcgtcagtac aaggcagcat ctcgatgtcc ggcaatgacc tgcagatcag ccccgatacg
     4621 ccgaaactaa cccgcacccg cggcgtgctg cactttaccc agcgtggctt tacgctggct
     4681 ggcgtgcaaa cccgaatgtt gggcggtgat gcccggctcg aaggtggctt gctgttgtcc
     4741 ggctctgaca actcacaccc gagcgccatt cgcgccgcag ggcttgccac tgccgagggt
     4801 ctgcgccagg ccaccgaatt gggtcttgtt gcgcggctgg ctcgccagat cagtggcagt
     4861 gccgcataca acgccagctt tggcctgcgc cgtggcgtgc cggagctgct ggtgaccagc
     4921 aatttgcaag gcatggcggc cagcctgccg tcgcctttga ccaagaatgc gcaaagcctg
     4981 ctgccgctgc gctaccagac cgcctcacct gtggcaccgc tgtctgccag gcgcacgccc
     5041 gcgccggtgc aagaccggct cacgctgaca ctgggcagtc tggtcagcct gacctatgaa
     5101 cgtgacgttt ctggcgccga accccgcgta ctgcgcggcg cgatcggcat tggtgccgat
     5161 accctggacg cggtgatgct gccggagcag ggcatcaacg ccaacatcca tttgccacag
     5221 ttcaacgtgg atgcctgggc tgacctgctg gcacaagtta ccgcagcgcc cctgggcacg
     5281 ccagatgcca gcaagcaccc ggaattggct tacctgccaa gcaacctggc ggtgcgtgcc
     5341 gacgtgctga catttggcac acgccagttc aaccgtctgg tgctgggtgc cggtcgagac
     5401 ggtttgctgt ggcgcgccaa tgtggattca gcagaactga atggttatct cgaataccgc
     5461 cagcctgacg cggccaatgc caacagtgcg ggtgcggtct atggccgact aacccgcctg
     5521 atcatcacgc ccagtatcgc caatgatgtt gaagcgctgc tggatactga atctgccagt
     5581 gtgccagccc tcgacctgct ggtggatgac tttgagctgc gcggcaaacg gctcggtcgt
     5641 ctggaggttg aggccgtcaa ccgtttgggc agcggaccag ccggtgtcaa cgaatggcgg
     5701 ctcaacaaac tcaacttgag catgcccgaa gccactttga cggccaacgg caactgggcc
     5761 cgactcaatg cccagaatgg tggcgcaccg ggtgccagca atcgcccgga gcggcaccgc
     5821 accgtgatga atttcaagct cgacatcgcc gatgggggca aactgctgaa ccgctttggc
     5881 atgaaagatg tggtgcgcca ggccagcggc aagatcgaag gccagttggc ctggatcggc
     5941 ccgccattca gcgtggatta cccgacgctg ggcggcgctg tcaccgtgaa tgtcttgtcg
     6001 ggacaattcc tcaaagctga tcccggtctg gccaagctgc tcggggtgct gagcctgcag
     6061 gccttgccgc ggcggctggt gctggacttt cgggatgtct tcagcgacgg ctttgcattt
     6121 gactttttgc gtggcaatgt ggcggtggaa aagggtctgg cgcgcaccaa caacctgcag
     6181 atgaaaggca tcaatgccgc cgtgctgatg gaaggcgtgg ccgatattga ccgggaaacc
     6241 caggacctca aggtggtggt ggtgcctgaa ctcaatgccg gcaccgcagc gttgattgcc
     6301 accgtcatca atccggcaat tggcctgggt tcgtttttgg cgcagctatt tttgagcaag
     6361 cccttgattg agtctgccac ccaggaattt cacgtgaacg gctcttgggc agaccccaaa
     6421 gtgaccaagg taaagcacat cacatcaacc agcaaggaaa aaccccaatg aaagtcgcag
     6481 tcatccagat ggtgtcgggc gctgatcttg ctgacaacct gcggcaggcc gcagagttgc
     6541 tcagccaggc agcctcgcag ggggccgagc tggtcctttt gcccgagtat ttttgtcaga
     6601 tgggtcagcg cgatgccgac aagttgtcga tttgcgagtc actcggtagc ggcccgattc
     6661 aacagttttt ggttgatgct gccaaagatc ttggcttgtg gctggccgct ggcacgctgc
     6721 cgctggccgt tgaccagatt ggtgggcaaa gccactctga gcgggttttc aacagcacat
     6781 tggtgttttc accggatggc agccgggtcg cacgctacga caaaatccat ttgtttcgtt
     6841 ttgacaatgg caccgagcac tacgacgagt cggtggtgct ggctgcaggc acccagccaa
     6901 ccagctttga tctgccatcg cgtgatggcc atatctggcg cattggcctg agcatctgtt
     6961 acgacctgcg ttttgccgag ctttaccgcc actatgccag cctgggggcg catttgctgc
     7021 tggtgcccag tgccttcact tacaccaccg gccagcacca ttgggacgtg ttgctgcgcg
     7081 cccgggcaat tgaaaacctg gcctttgtgg ccgccgccgc tcaaggtggt cagcatgaca
     7141 atgggcgcca aacctggggc cattcgatgc tggtggaccc ctggggtctg gtgttggcac
     7201 agcgaccacg ggatgccggt gtggtggtgg ctgagcttga cttcggcttg ctggaaagct
     7261 gccggagccg cttgccggct ttgcagcatc gcgttttatg agccggatgc cgcttttttt
     7321 cggcgctggg ctggtggccc tggcactttg gctgacatct tgcggcaatg tgccgctgca
     7381 tgtgccgcaa acacctgacg cagcgcggcc aacaccgcct gtcccgatgc ctgacgcggc
     7441 cctgccgaga acggtattgt tgcgcaacaa gagccaatgg gtggcggtgc cctggccgga
     7501 tctgcccggc tttgaggacg atgcactttt tgaggcctgg aatgcgttca tcaagagttg
     7561 cgagcgtccg gcaccagtgt ttgccccgtt gtgcccggag attcggcgtt tgagcattgg
     7621 caccgatgcc cagcagcggg aatggttgct gcagcgcctg caaccctatc gggttgagcc
     7681 cttgcaggcc gaagcctcaa cggggctgct gacggcctat ttcgagccag agtttgaggc
     7741 caggcgccag gctggcgctg gtttttcctt cgctctgcac caggctccgg caggcctgac
     7801 ggctggcaga ccctggttta cccggcaaga aatggaaacc ctgcccgccg cgcaggcggc
     7861 cttgcgtggc cgtgaaatca tctatctggc agacccggtg caagcgctga tcatgcaaat
     7921 tcagggctct ggcagagtgc gcgtaaccga gcccgatggc agtcagcgtc ggctgcgcct
     7981 ggcctatgca gccaacaacg ggcgacccta ccaaagcatt gggcgctggc tgctcgacca
     8041 gggtgaactg cgtgatgcgt cgtggcctgg cattcgggcc tggctcatgc aaaacccgca
     8101 gcgcaccgcc gaactgctgt ggcgcaaccc cagagtggtg tttttcaagg aagagccgtt
     8161 gaatgacttt gatgccgcct ttggtccacg cggtgcccag ggcgtgccgc tgacaccggg
     8221 tcgctcgatt gccgtggacc ccgacagcat tccgtatggc acaccagtct ggctggcatc
     8281 ggttggaccc agcttgtcac tgcagcggct ggtgctggca caagacaccg gcagtgccat
     8341 caccggtgcc ttgcgggctg actattttgt cggctggggc gcgcaggcgg gcgaactggc
     8401 cggacgcctc aaacagccgc tgagactttg ggttttatgg cccaaacagc aaagataacg
     8461 ttaccattga ccctatcgac aatctgttct taacaaagaa gaagccatga acaaatcaac
     8521 catgcgcgat attgacgagc gaaccaatct ggctggtaac agcatgtttg agttgctgct
     8581 gttccgtttg ggtgaagccc ctggcactga ccggcgcgag ctgtttggca tcaatgtttt
     8641 caaggtgcga gaaatcctgg tcatgccgga aatcactgcc atggtgaatt cgccaccgtc
     8701 ggtgatgggg gttgccaaca tccggggaca aatgatctcg gtgatcaatc tgccgcagat
     8761 tgttggcacc aacccgacca agggcctggg catcttgctg gtgaccgaat tcgcccgcac
     8821 cacacaggct tttgcggtcg aagaagtcaa cgaaatcgtc cggctggagt ggaagcatgt
     8881 gctttcagcc gaagtgcgtg gcggcggtct ggtgaccagc attgcgcggc ttgacggtgc
     8941 ggccgaaaac acccggctgg cgcaggtgct tgacgtggag cagatcttgc gcgatgtttt
     9001 cccggagcag caacaagttg agcttgaacc cggcaaccag ttgcatgtgc ccgccggtac
     9061 ggtgatcctg gcggccgacg attcggcggt ggcccgcatg atgatcgagc agggcctcaa
     9121 gggcatggaa gtaccctaca tcatgaccaa gagcggccag gaagcctggg accgcctcaa
     9181 agccatggcg gcagaggccg agtcgcaggg caaaaccatc cgcgacaaga tcgcgctggt
     9241 gctg
//
LOCUS       DAOSLL010000117         9229 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7134_length_9229_cov_5.123719, whole genome shotgun sequence.
ACCESSION   DAOSLL010000117 DAOSLL010000000
VERSION     DAOSLL010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9229
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7134_length_9229_cov_5.123719"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLB25_06945"
     CDS             complement(<1..299)
                     /locus_tag="PLB25_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE41352.1"
                     /translation="MIEAATWVPTPKSALAQRPQDENRARQSGFTLIELIIFIVVVSA
                     GLAGILSVMDVTVKSSGDPVVRKQVIAIAESLLEEILLKDYCDPDTVNRATTPPA"
     gene            complement(292..879)
                     /locus_tag="PLB25_06950"
     CDS             complement(292..879)
                     /locus_tag="PLB25_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE41353.1"
                     /translation="MNERPKRLAATAGFGCQRRGWARPRQQHGFTLVELVMVIIMLGV
                     LAVFAAPRMFNSADFHARGLHDETLSLLRYAQKAAVAQRRMVCVTFDTSITLNTAALT
                     LENPTPVASPMPVVNCSVNLVGPKGDAPATVTAKSGTGYSAIKTGTASVTGLIFNGLG
                     QPVSAMRVVLADSSTVEITNAAGITIEAVTGYVHD"
     gene            complement(907..1512)
                     /locus_tag="PLB25_06955"
     CDS             complement(907..1512)
                     /locus_tag="PLB25_06955"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOE41354.1"
                     /translation="MRLAFKPRLTAVDYLAGEQDSDIRHEYVDGEVYAMAGAGESHNL
                     IALNVASRLRNLVRGGPCRVFISDMKLQVAAWDAFYYPDVMVVCDASDTQTHFKRSPS
                     LVVEVLSPATESIDRREKMLAYRTLPSLREYLLIAQDRRHVELYRRADSGTWFLAALP
                     EGADVPLECVNASLTLDDVYEDVKFMPNVPTALNDSASIAS"
     gene            complement(2041..2217)
                     /locus_tag="PLB25_06960"
     CDS             complement(2041..2217)
                     /locus_tag="PLB25_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41355.1"
                     /translation="MAWLSTSPRAEAVIRCPAVAVDFQHRSLAWCAGKGGFPAARPGE
                     LAGAPKLSNLTSTD"
     gene            2578..2893
                     /locus_tag="PLB25_06965"
                     /pseudo
     CDS             2578..2893
                     /locus_tag="PLB25_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831436.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
     gene            3298..3723
                     /locus_tag="PLB25_06970"
     CDS             3298..3723
                     /locus_tag="PLB25_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone"
                     /protein_id="HOE41356.1"
                     /translation="MATAKKPAASKVAPETKAPAARKAAPVEKVTLAKAAAPAKKTAA
                     AKKTVAKKTAPAKVVAAAPSVSEKSAAPAKKAASAAKVSVKTSATKLATKENAAPAKK
                     SAATKTAVNEATKAPAAKTTTATKLNPQAPWPFPTGSKP"
     gene            complement(3787..4716)
                     /locus_tag="PLB25_06975"
     CDS             complement(3787..4716)
                     /locus_tag="PLB25_06975"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HOE41357.1"
                     /translation="MASEKESSDTVKVDSPPIPETEPTEENLAAQQLAEIQPALQAEV
                     NEVSDLEAEVAAADEPAPEPFEQGEAGIDQLLRVEAISDGAPTLSLDSEGSSESSALN
                     EAAAEDPAQEHVSEETVSPADITSAPTARESTPAEVSFLVGADRQSVWCTGLGRVALS
                     FAMVLLAAALLLQWLYWDRDRLAATNPHWKPLLQQLCRPLNCSVRALQRIESIAIGAA
                     AFNQLSADSYRLSFSIKNLSGAELAMPSLELTLTDTQDQVIVRRVLSRQDLFVVSDDL
                     SAGSELPVTVDMHMTQEEATPRIMGYRLLAFYP"
     gene            complement(4753..4857)
                     /locus_tag="PLB25_06980"
                     /pseudo
     CDS             complement(4753..4857)
                     /locus_tag="PLB25_06980"
                     /inference="COORDINATES: protein motif:HMM:NF025096.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
     gene            complement(4876..5781)
                     /gene="prmA"
                     /locus_tag="PLB25_06985"
     CDS             complement(4876..5781)
                     /gene="prmA"
                     /locus_tag="PLB25_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HOE41358.1"
                     /translation="MFELRLMCPEDRVERLSDALEVLDALSVSVEDADAQTDAEQALF
                     GEPGLPPPKDGWQRSRVLALFAEREAAQTARQLLQLQDFFTGCQFLGIEHVPEQDWVR
                     LTQSQFAPVEITPDFWIVPTWHDPPAQARVVIRLDPGLAFGTGTHPTTRMCLRWIADQ
                     GRLGATSAQPVLGHVLDYGCGSGILAIAAAKFGATSVVAVDIDAAAIASALCNAQANH
                     VVLNAGLPEVASGVYQVVLANILASPLKVLAPLLWSHVASGGFLVLAGILERQAEEIR
                     AAYAPFCHLSVSDSEDGWILMTASR"
     gene            complement(5795..7144)
                     /gene="accC"
                     /locus_tag="PLB25_06990"
     CDS             complement(5795..7144)
                     /gene="accC"
                     /locus_tag="PLB25_06990"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HOE41359.1"
                     /translation="MFKKILVANRGEIALRIQRACRELGVKAVMVYSEADREAKYVKL
                     AEEAVCIGPAASSLSYLNMPAIISAAEVTDAEAIHPGYGFLSENADFAERVERSGFQF
                     IGPTPESIRIMGDKVSAKQAMIKAGVPCVPGSEGELPDDPVQIKRIAKSVGYPVIIKA
                     AGGGGGRGMRVVHTEAALINAVAMTKNEAGIAFGNSAVYMEKFLQNPRHVEIQILADK
                     YKNAVYLGERDCSMQRRHQKIIEEAPAPGIPRKIIDRVGDRCVAACKKIGYRGAGTFE
                     FLYEDGEFYFIEMNTRVQVEHPVTEMTTGIDIVKTQIMVAAGERLPFTQRQIQIRGHA
                     IECRLNAEDSYKFIPSAGRITMWHPPGGPGVRVDSHAYTNYYVPPNYDSMIGKIIVHG
                     DTRDQALARMRTALGETVVEGINTNIALHRDLMIDAKFMLGGTNIHYLEGWLADRTR"
     gene            complement(7160..7612)
                     /gene="accB"
                     /locus_tag="PLB25_06995"
     CDS             complement(7160..7612)
                     /gene="accB"
                     /locus_tag="PLB25_06995"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HOE41360.1"
                     /translation="MDLRKLKTLIDLVSESNVSELEITEAEGKVRIVKGGGMLAQQYV
                     QAGVPAAAMPVVAAPLVSDLATPAAVAETGHTTKSPMVGTFYRSASPGAKAFVEVGDT
                     VKEGDTICIIEAMKILNEIEADKSGTIKRILAENGQAVEYGQPLFIIE"
     gene            complement(7733..8284)
                     /locus_tag="PLB25_07000"
     CDS             complement(7733..8284)
                     /locus_tag="PLB25_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOE41361.1"
                     /translation="MSASPPVVSRRGWLAIAAGVAVAAGGTGLALRRSWQQSRELSAV
                     ETLLWQQTFSQVDGTVLAMAAFKGKPLVLNFWATWCPPCLEELPLLNAFFSENKAKNW
                     QVLGLAVDQALPVKRFLAHSPLSFPVALAGFPGVELSRSLGNLSGGLPFTVVFGSAGE
                     VQHRKMGKLNSTELQAWSRLQGS"
     gene            complement(8281..8874)
                     /locus_tag="PLB25_07005"
     CDS             complement(8281..8874)
                     /locus_tag="PLB25_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41362.1"
                     /translation="MFETKFEIAALAARLVVEEGLEYGPAKRRAARALGSSGRKPLPG
                     NDELEDAVRDYIAVFCAESQAAELLALRQLALVWMDRLAMFRPHLGGSVWRGTANRLS
                     DIYLQLFCDDSKGAEIALIDKQVKYVARSITGFQGETVDALSVHAFCPGLQEEVGVHL
                     MIYDFDAVRGALKPDARGRSLRGDARALRQLLGEVLP"
     gene            8963..>9229
                     /locus_tag="PLB25_07010"
     CDS             8963..>9229
                     /locus_tag="PLB25_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOE41363.1"
                     /translation="MHLHILGICGTFMGGLAALAREAGHKVTGCDSGVYPPMSEQLRA
                     LGIDLVEGYGADQLVLKPDIFVVGNVISRARMPDGSVKFPLMEAI"
BASE COUNT         2060 a         2823 c         2511 g         1835 t
ORIGIN      
        1 caggccggtg gcgtggtggc ccggttgacg gtgtccggat cgcagtagtc ctttagcaaa
       61 atctcttcga gcagcgactc tgcaatggcg atgacctgct tgcgcaccac cgggtcgcca
      121 ctggatttga cggtcacgtc catcaccgac aggatgcccg ccaggccagc gctgaccacc
      181 acaatgaaga tgatcagttc gatcaaggtg aagccgcttt ggcgcgcccg attctcgtcc
      241 tgcggccttt gggccaacgc cgatttcggg gtcggcaccc aggtggccgc ttcaatcatg
      301 cacatagccg gtaaccgctt cgatcgtgat gcccgcggca ttggtgattt cgaccgtgct
      361 gctgtcggcc aatacaacac gcatagcgct aacgggttgc cccagaccgt tgaaaatcaa
      421 accggttacg ctggcggttc cggtcttgat ggcgctgtag ccggtgcccg attttgctgt
      481 cacggtggcg ggagcgtcgc cctttgggcc gaccaggttg acgctgcagt tcacaaccgg
      541 catgggtgaa gcaactggag tcgggttctc cagcgtcagg gcggcggtgt tgagcgtgat
      601 ggaggtgtca aaagtgacac acaccatgcg ccgctgcgct accgctgcct tttgcgcata
      661 acgcagcagc gaaagcgtct cgtcatgcaa tccgcgcgca tggaaatcag cgctgttgaa
      721 catgcgcggg gctgcaaaca ccgccagcac gcccagcatg atgatcacca tgaccaactc
      781 aaccagcgtg aagccgtgtt gttggcgcgg ccgggcccag ccccggcgtt gacagccaaa
      841 accagccgtc gcagccaagc gcttgggcct ctcattcatt gctttgaatc gccaaattgc
      901 tattaattac gaagctattg acgcactatc attaagggct gtaggcacat ttggcataaa
      961 tttgacatcc tcatacacgt catcgagcgt caggctggcg ttgacgcatt cgagtggcac
     1021 gtcagcgccc tcgggcaaag cagccaaaaa ccaggtgccg ctgtcagcgc gccggtaaag
     1081 ctcaacatgg cgcctgtcct gggcgatcag caggtactcg cgcagactgg gcagggtacg
     1141 gtaggccagc attttttcgc gccggtcgat tgactcggta gcaggtgaca gcacctcgac
     1201 caccagactc ggacttcgct tgaaatgggt ttgcgtgtcg ctggcatcac aaaccaccat
     1261 cacgtccggg taatagaagg catcccacgc cgccacctgc agcttcatgt ccgagataaa
     1321 cacccggcac gggccacccc gaaccagatt gcgcagccgc gaagccacgt tcaaggcgat
     1381 caggttgtgc gactcgcccg caccagccat ggcatagacc tcgccatcga catactcatg
     1441 acggatgtca ctgtcctgtt caccagccag atagtcaacg gcggtcaggc ggggcttgaa
     1501 ggcgagtcgc atcgggattg atctccgcaa aaggatgatt gctgcgcgaa actgccatta
     1561 tgttgcaagc cttcggccgg atgccaatcg gtctgaccgt gcgccgacgc cccgtgactg
     1621 ataccgaaaa gacgacgcag ccgtggccca tgacggcgca gagacccgcc tgaatcaaaa
     1681 tttggtcaac cgagtctggc ctcttgatcg gctctttatc cagggtgaac gcgtcatgac
     1741 aggaatgccg cgcgcagttg cagatctggg tttggatgcc aaggcggcac cggatgcgtg
     1801 agttggacaa caagggctta ccgaattggg ttctgtcccc aattctgctg gccaccaccg
     1861 caagctgcct gagagcatcg cggagtggcg cggctttgtt tgaaaagttc gcttgtagca
     1921 cttgtccagc aagcgctggc agctattcga atgctcaatt gcgagtggag acgatggcac
     1981 gacgcttcgt tcagccgacg catatccgga cttccagccc gcaatacaac gccaccgact
     2041 tcagtcggtg cttgtcagat tggaaagctt gggggcacct gcaagctcgc ctggtcgcgc
     2101 ggccggaaag ccacctttgc ctgcgcacca tgccagcgac cgatgctgaa aatcaaccgc
     2161 caccgcaggg caccggatga cggcttcggc gcgtggcgag gtcgaaagcc aagccatgtc
     2221 agatgccatg gatgcagcgg ctagcgcaac agcacctgct tggcggcatc agcggcaacc
     2281 actgttgaat tgagtggtgg caatgaccga atggcaaatc ccgccagcaa tggccgaccg
     2341 ttgacactga tgcgatgttg atgcacaaca acgtgcattt gatctgccca aagccaactt
     2401 aagcgctttc cattctggcc ctgcgctccc ataattcgac aatcggaaat ctatacgttg
     2461 tcatgggaca aagaagtcat atctgctgcc agtttggcca tcccgcgtgg tcagtcgaat
     2521 cccccgttgg tcgcgctatt tggcggcccg gtcgcaacgt tttccgctac cgccgggttg
     2581 gtggttgcag ccattgcaca gttggtcggg aaaactgcgg acacgcgcat catctgtggc
     2641 aaggccgatg ttgaccaatc ggtgccgctc aaggacaagt gggcatgggg tagggtttta
     2701 tcgacttgcg ccaaccgctg gatgccccaa gaggtcaaca acgcccgaga tcggctattg
     2761 gaatgattct ctcgggttga gcgaaagcga gcgccgcctt gtcaagcatg agttccggtt
     2821 tttcgtcacg gcagattcac tggcagccaa cagcattgaa ttgaacatcg accaccacat
     2881 cacagcaccc aaggcggccc tttacaccca tgcctacctg catattgtcg atgtctcgca
     2941 gcttgctgcg acttgcaggg cgcagccatc ccacgaggct ggtcagcttg gaaccacggc
     3001 tttcgccctg cccagacgtg ccgccagcag cccacttggt tgcgtccaac accggggtcc
     3061 agcatgatcg acttgagaat cgaacagatt ttttcagacc agcccaaagg ggcactctca
     3121 tgggacccat gccctgaaat ggcacaagca atttcacaag ctgttagccc gcgttggtgc
     3181 gggtgccagc ttgtgcgccc aagtttatgc tgctgggatc atgcccaaca ccatgaatcg
     3241 ctacatgccc atttattcgc atgaagtgaa atgtgtaatt tgatcaagga gattcgaatg
     3301 gcaactgcaa aaaaaccggc cgcaagtaaa gtggctcctg aaaccaaggc ccccgctgcc
     3361 cgaaaggcag ctcctgtcga gaaagtcacc ctggcaaaag ctgcggctcc tgcaaaaaag
     3421 acggcagcgg ccaagaagac agttgcgaaa aaaactgcgc cagcaaaagt agtcgccgcc
     3481 gcaccgtcag tttctgaaaa atcggcggca ccagcgaaga aggccgcatc ggccgcaaaa
     3541 gtgtcagtca aaacgtcggc aacaaagctc gcgaccaagg aaaacgctgc accggcaaag
     3601 aaatctgcag caacaaaaac ggctgtcaat gaagccacca aggcccccgc cgcaaagacc
     3661 accaccgcaa caaaactcaa cccgcaagct ccatggccct ttcctactgg ttcgaagccc
     3721 tgaacttttc ccgttgcacc tgcccaccca gcccgcgttg cctcggtgca ccgggctttt
     3781 cgccaatcag gggtagaagg ccagcaatct gtatcccatg atgcgcggtg tcgcctcttc
     3841 ctgagtcata tgcatatcca ccgtcacggg caattccgac cccgccgaca aatcgtcgga
     3901 tacgacaaac aagtcctgac gcgacaacac ccgccgaacg atgacctggt cctgggtatc
     3961 ggtcagcgtc aattcgaggc tgggcattgc cagctccgca ccagacaggt tcttgatgct
     4021 aaaactcaac cgataactgt cagcactcaa ttgattgaac gccgcagcgc cgatggcgat
     4081 ggactcaata cgctgcagcg cccgcaccga gcaattcaag ggtcggcaga gctgttgcaa
     4141 tagtggcttc caatgcggat ttgttgcagc caggcgatca cggtcccagt aaagccattg
     4201 aagcaacaac gcagcagcca atagaaccat agcgaatgac aaggcaaccc tgccaagccc
     4261 ggtacaccag accgattgcc gatctgctcc aaccaggaac gacacttccg ctggtgttga
     4321 ctcacgcgca gttggtgccg aggttatgtc ggcaggggag acagtctcct ctgaaacatg
     4381 ttcttgcgcc gggtcttcgg ccgctgcttc gttcaaagct gacgattctg atgacccttc
     4441 gctatcgaga cttaaagttg gcgcgccatc tgaaattgcc tccacacgaa gcaactgatc
     4501 aatgccagcc tccccctgtt caaagggctc gggtgcgggt tcgtcggccg ctgcgacttc
     4561 ggcttcaagg tcagatacct cgttaacctc ggcctgcaag gcaggctgga tttcggcaag
     4621 ctgctgcgct gcaagatttt cctctgtcgg ctcggtttcc gggatcggtg gtgaatctac
     4681 cttgaccgta tcggaagact ccttctcgga ggccatggcc acctcgatca aatgctctga
     4741 agcatcaaat atctcggcac actgaccgca tctgacccaa ccctgcgaaa tccgcaactg
     4801 atccggtacc agcttgtaca gcgtttcgca ggccgggcag cgtgtcaaaa ggctcatgga
     4861 atttggcgac agcggtcagc gtgaggcggt catcagtatc cagccatctt cactgtcaga
     4921 cacactcaag tggcaaaagg gcgcatatgc agccctgatt tcctcggctt gacgctccag
     4981 gataccagcc agcaccaaaa agccaccgga tgcaacatgc gaccacagca aaggtgccag
     5041 caccttcagg ggactcgcca gaatattggc aagaaccact tggtaaacgc cgctggcgac
     5101 ctcaggcagg cctgcattca acaccacatg attggcctgc gcattgcaaa gcgctgacgc
     5161 aatggccgca gcatcaatgt caacagccac aacgcttgtc gcgccaaatt tggctgcggc
     5221 aatggccaag atgcccgagc cacagccgta atcgagcacg tgtcccagga caggctgggc
     5281 gctggtcgcc cccaagcgtc cttggtcggc gatccagcgc aggcacatgc gcgtggtggg
     5341 atgggtgccg gtgccaaagg ccaggccggg gtcgagccgg atcaccaccc gcgcctgcgc
     5401 gggtggatcg tgccaggtcg gcacaatcca gaagtcaggc gtgatctcca ctggggcaaa
     5461 ttgcgactgc gtcagtcgaa cccagtcttg ctcaggcaca tgctcgatgc ccaaaaactg
     5521 acagccggtg aaaaaatcct gcaactgcag caattggcgg gcagtttgcg ccgcctcacg
     5581 ctcggcaaac agcgccagca cccttgagcg ctgccagccg tctttgggcg gcggcaagcc
     5641 cggctcgcca aacagcgcct gctcggcatc ggtctgcgca tcagcatctt cgacgctgac
     5701 gctcaaggca tcaagcacct caagcgcatc actcaggcgc tcaacccgat cctccgggca
     5761 catcaggcgc agttcgaaca tgttggatag ggccttagcg ggtgcggtca gccagccaac
     5821 cttcaaggta atggatattg gtgcccccca gcatgaattt ggcatcaatc atcaggtccc
     5881 gatgcaaggc aatattggta ttgattcctt ctaccaccgt ctcgcccagc gccgtgcgca
     5941 tgcgcgccag agcctgatcc ctggtgtccc catgcacaat gatcttgccg atcatcgagt
     6001 catagttcgg cggcacatag taattggtat aggcatggga atccacccgc actcccgggc
     6061 caccaggcgg atgccacatg gtgatgcgcc cggccgaagg gatgaatttg taggaatctt
     6121 ctgcatttag cctgcattca atggcatgac cacggatctg gatttgccgt tgggtgaacg
     6181 gcaatcgctc gcccgctgcc accatgatct gggtcttgac gatgtcaata ccagtggtca
     6241 tctcggtcac cgggtgctcg acctgtaccc gtgtgttcat ttcaatgaag taaaactcac
     6301 cgtcttcgta taaaaactca aaagtgccgg caccgcgata gccgattttc ttgcaggccg
     6361 ccacgcagcg gtcaccgacg cggtcaatga ttttgcgggg gatgccgggt gccggtgcct
     6421 cttcgatgat tttttgatgg cgccgctgca tcgagcagtc acgctcgccc agatagaccg
     6481 cgtttttgta tttgtccgcc aggatctgga tctcgacgtg gcgcgggttc tgcagaaatt
     6541 tctccatata gacggccgag ttgccaaagg cgatgccggc ttcattcttg gtcatggcca
     6601 cagcattgat cagcgccgct tcggtatgca ccacccgcat gccgcgtcca ccgccgccgc
     6661 cggctgcctt gatgatcacc gggtagccaa cgctcttggc aatgcgcttg atctgtaccg
     6721 ggtcgtccgg caactcacct tcagaacctg gtacacaggg cacaccggcc ttgatcatgg
     6781 cttgcttggc cgacaccttg tcgcccatga tccggatcga ctccggggtt gggccgatga
     6841 attgaaagcc actgcgttca acccgctcgg caaaatcggc gttctcgctc aagaaaccat
     6901 agccagggtg aatcgcctcg gcatcggtga cctcggccgc cgaaatgatg gctggcatgt
     6961 tcagatagct caggcttgac gcggccgggc caatgcatac cgcttcttcg gccaacttga
     7021 cgtacttggc ttctcggtcg gcttctgaat acaccatgac agctttgaca ccgagttcac
     7081 ggcaagcccg ttgaatgcgc aatgcgattt cgccacggtt ggcaaccagt atttttttaa
     7141 acatggatct cgcctgacat cattcaatga tgaacagggg ttggccgtat tccacggcct
     7201 ggccgttttc ggccaggata cgtttgatcg tgccggactt gtccgcctca atctcgttga
     7261 ggattttcat ggcctcaatg atgcaaatgg tgtcgccctc tttgactgtg tcacccactt
     7321 caacaaaggc cttggcacct ggggacgcgg aacgataaaa cgtgccgacc atgggggact
     7381 tggtggtgtg gccagtttca gccaccgccg ctggcgtggc aaggtcgctg accaagggtg
     7441 cagcaacgac cggcatggcc gccgcaggaa caccggcctg cacatattgc tgggccagca
     7501 taccccctcc tttgacaatg cgcaccttgc cctcggcttc ggtaatttca agctccgaga
     7561 catttgactc tgacaccagg tcaatcaggg tcttgagttt tctaaggtcc atgtggtctc
     7621 caacgaacaa agggaaaatt gggctaaatg aacttaaatg ctgttaaagc tatatcaaac
     7681 aaccgctaaa gtagcccaaa agcttctatc ttacagcgca atgcaagggc catcatgacc
     7741 cctgcaagcg actccaggcc tgaagttcgg tcgagttcag cttgcccatt ttacggtgtt
     7801 gaacctctcc cgccgagcca aacaccaccg taaacggcaa accaccactc aagtttccaa
     7861 gtgaccggct cagctcaacg cctggaaacc ccgccagcgc aaccggaaaa gacagcggtg
     7921 aatgggccag aaaacgcttc accggcaacg cctgatccac cgccaatccc aggacttgcc
     7981 agtttttggc cttgttttcc gaaaagaagg cattcaaaag cggcagttcc tccagacagg
     8041 gtgggcacca ggtggcccag aagttcagta ccagcggctt gcctttgaac gcggccatgg
     8101 ccagtaccgt gccatcaacc tggctgaatg tctgctgcca gagcaaggtc tccaccgccg
     8161 aaagctcccg ggattgttgc caggaccgcc gcaacgccaa cccggtgccc ccggctgcca
     8221 cagccacccc ggctgcgatc gccagccagc cgcgccgcga taccaccggt ggcgaagcac
     8281 tcaaggcagc acctcaccta acaattgccg caaggcccgg gcatcacccc gaagtgagcg
     8341 cccccgcgca tcgggtttca gcgcaccacg tactgcatca aagtcataaa tcatcaaatg
     8401 cacaccaacc tcctcttgca gtccagggca aaacgcatgg acactcaggg catcaaccgt
     8461 ttcgccctga aaaccggtga tactgcgcgc cacatacttg acctgtttgt ctatcaacgc
     8521 aatttcagca cccttgctgt catcgcaaaa aagctgcaga tagatatcag acaggcgatt
     8581 tgcggtgcca cgccagaccg aaccgcccag atgggggcga aacatcgcca ggcgatccat
     8641 ccagaccagt gccaactggc gcaaggccag caactcggca gcttgcgact cggcacaaaa
     8701 gaccgcaata tagtcccgca ccgcgtcctc aagctcatcg ttacccggca agggcttgcg
     8761 gcccgatgag cccagcgccc tggccgcgcg gcgcttggcc ggaccgtatt ccagcccctc
     8821 ttctaccacc agacgcgcag ccagcgccgc gatctcaaat ttggtttcaa acatcagcag
     8881 ccgattttgc ccgcatttgc ttgccctcac cttgccaatg agatgcggca ggctggtttg
     8941 cctgcgcctc taaaatccgt ccatgcactt acatattctt gggatttgcg gcaccttcat
     9001 gggcggtctg gcggctttgg cgcgcgaagc cggacacaaa gtcacgggtt gcgacagtgg
     9061 cgtttaccca cccatgagcg agcaactcag ggcgctggga attgacctgg tggaaggtta
     9121 tggtgccgac caattggtac tcaagccaga cattttcgtc gtgggcaatg tgatcagccg
     9181 cgccagaatg cccgacggca gcgtgaaatt tccgctgatg gaggctatt
//
LOCUS       DAOSLL010000118         9084 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7249_length_9084_cov_4.602060, whole genome shotgun sequence.
ACCESSION   DAOSLL010000118 DAOSLL010000000
VERSION     DAOSLL010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9084
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7249_length_9084_cov_4.602060"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="PLB25_07015"
     CDS             complement(<1..760)
                     /locus_tag="PLB25_07015"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOE41364.1"
                     /translation="MKTLMTLLTLFALMLAGCGKQDNAAAPPDPANTLSVLAGSELKD
                     IEPLLPALTQATGITLQMRYVGTLEAVEKLQAGEAVDAAWLASNRYAMLVPDARARIV
                     ASERTMLTPVVLGLKDRKATELGWKNNPNITWKDIAQAAAQGQFTFGMTNPASSNTGF
                     SALLGLAAALSGKGDALEEKDIDAQRLAAFFKAQRLTAGSSGWLAEAYLKGPGKVDGI
                     INYASTLLSLNRNPALAEKLVLIYPQDGIATADYP"
     gene            complement(868..1974)
                     /locus_tag="PLB25_07020"
     CDS             complement(868..1974)
                     /locus_tag="PLB25_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41365.1"
                     /translation="MEVRRVLGPALAAILAIGIGAAIYVSYGRQSTEQAAKDAAAAMV
                     DVKGMIGSEKESFFADPKVIERLAAKGLRVRVEKVGSREIAARDSKGYDFGFPAGAPA
                     AITLQDKAKAKQVFPTFFTPMAIASWQLLIPVMENEGIVKKTDGTYYIIDMKRLLGLS
                     EKGVRWRDLKGNTAFATSKSILISSTDVRKSNSAAMYLALASYVVNDSNVVDDQAQAS
                     KVLPFVTSLFLRQGLQESSSAGPFEDYTTMGVGKAPLVMIYESQFLEYQSRRAQPNPD
                     MVLLYPQPTLYTKHILVPFTENGSKLGELLANDAELQRMAANYGYRTAAPALFAEFLK
                     DRKLSAPVTLVDVIDPPSYEILERMIQAIEKKFQ"
     gene            complement(2163..3950)
                     /locus_tag="PLB25_07025"
     CDS             complement(2163..3950)
                     /locus_tag="PLB25_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOE41366.1"
                     /translation="MALALMMLIPLLEIMLRPVMGRGVENAAVLVQHLGLVLAMLGAL
                     AAERHGHLSSLGNGLASWGGERLQVGVKAFAQGSAALVCGVMALASWHLVAVEIDAAQ
                     NLAYGMPTWWIQLLMPLGFGLLGFKLGARCADRVGIKLLAALVLPAAGFWLGNIYDGS
                     PLPLWPMLLILVAALLGGAPIFAVLGGLALALFWQQGQPLASVALSHYQITVNASLPA
                     LPLFTLAGLVFARTGAAARLGALFVALFGGGMSGTVVATAVLCSFFTAFTGGSGVTIL
                     ALGGLLLPLLRNVGLPEQRGISLVTSASALGVLLAPSVPLIMYAIMARVPINTLFLAG
                     VIPAFVMVGVLLLLGGYLQRAGLMPAAVIPANTKVSSHFGSGIGATLWAAKWELLAPV
                     MAIGSLVSGLATPTESAALTAAYAIATQALAHRELGWRVLGNAFSDCTQIIGGVMLIL
                     GMALGLTNYLVDSGLPNDAIDWVQGMIPNKYLFLLALNVFLLAAGALMEIYAAIVFLV
                     PLLLPVALSYGIDPVHFGIIFLANMEMGFLAPPAGMNLYFASAMFDKPIHYVARAVLP
                     AVLAMFLGTLAISWIPVLSTGLPRLLTGP"
     gene            complement(4166..5197)
                     /gene="dctP"
                     /locus_tag="PLB25_07030"
     CDS             complement(4166..5197)
                     /gene="dctP"
                     /locus_tag="PLB25_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOE41367.1"
                     /translation="MTYFPTLRRQILSICLAALAATSVPAAHAATQQLRIGTLAPKNS
                     LYHRQLQAVGEAWRSAQGGDAKYLIYPDGSQGGEAELARRMRIGQLQGALISVVGLRE
                     IEPAITALQSLPLLFKNWDEVDYVREKMRATMERKFFDKGFVVLAWGDSGWVRFFSKE
                     PAFKPTDYLKMKFFAWGAESEQQEIMKSLGYTPVPLETSDILPAIQTGMISVVPSTPY
                     FALASQIYNTAPNMLEINWAPIVGALVITKKAWDDMSPSAQQVVRNAGEKAGLAMRTQ
                     ARLEVDEAVAAMKQRGLVVNKPNPEQMKEWSALADKLYPRIRGHMVPADTFDEVFMHL
                     KAFRATPGR"
     gene            complement(5206..6045)
                     /locus_tag="PLB25_07035"
     CDS             complement(5206..6045)
                     /locus_tag="PLB25_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HOE41368.1"
                     /translation="MALTACSPRQLILQEAASALATQGNAPEDDLVLAREASAFYLKL
                     SEAVLRETPGNLPLAAAVAAGFTQYAYAFVQLEAERTEAKDANAAQRLRERAARLYLR
                     GHRHAMTALENRHPGFAKALANLAQIDGLHLSPEEVDLAYWAAASWGGLIALSKDQPN
                     SVADLPLAIRLARLAYQTAPEHGQGALATLMGSFEAARPGGSRQQAARYFDVAITLGA
                     GKSAGAFVAKAESIALPAGDRPAFDALLRQALTASASRADLQSAVMRERALWLLATAD
                     DLF"
     gene            complement(6081..6371)
                     /locus_tag="PLB25_07040"
     CDS             complement(6081..6371)
                     /locus_tag="PLB25_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41369.1"
                     /translation="MPQDAEITFGAPVASSEDNSGDEYESVGDHHRAAARHFDAAARH
                     HLAAADAEEEGNSDTADRHAYLAYRQRLNGVQYAETALMDSERVADVIESNL"
     gene            complement(6581..7723)
                     /locus_tag="PLB25_07045"
     CDS             complement(6581..7723)
                     /locus_tag="PLB25_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011300190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HOE41370.1"
                     /translation="MRMDNIGKKKTVLVVDDVPENIAIANSVLKDSYKVQTALDGASA
                     LDLVLGARRRPDLILLDIQMPGMDGFEVCRRLKTDPKSRSIPVIFLTSLADQMDEARG
                     FVVGAVDYIHKPFSPAIVKARVATHLSLKEAHDQLENQNQLLEEKVKDRTAEIALVQG
                     VTIGAMAALAEARDNETGNHINRTQRYIERLAWGLRSHTRFVKVLTPQVIESLYRSAP
                     LHDIGKVGVPDRILLKPGSLDAEEFEIMKTHASIGAVAIARADREMGSTGNSFLRYAH
                     EIALHHHEKWDGSGYPHGLIGEAIPVSARLMALADVYDALISKRVYKPAYSHDKAVTI
                     IMKGRGQHFDPDIVDRFAGIQDEFQKVAHSFRDEGDAEPQPGPVAG"
     gene            complement(7677..>9084)
                     /locus_tag="PLB25_07050"
     CDS             complement(7677..>9084)
                     /locus_tag="PLB25_07050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE41371.1"
                     /translation="KVRQVDPSDPYDVVFLDWKMPELDGFDVARRVRDDPQLQHQPKL
                     VLVTAYGRAGLEGELAQSMMDAILAKPITPSTLHDTLLELHTGHTRQHRLPPPPLPSL
                     QGYRLLLVEDNDINQQIAVEILESAGAEVAIARNGLEAVHQVLHRHKSFDLVLMDLQM
                     PEMDGYQATAQIRAHTAYDALPIIAMTAHAMEEERQRCMASGMNDHVSKPIDPQTLFA
                     TLLRWLPTTPPRRPAPIKPEQQGHIGNTILADTDLPPIAGIDLADGLQRLSGNRVLYH
                     RLLCQFAQTRHGMGDEMADALAQDRREVAALVAHSLKGLAANLGAGRLAADAAAVDVA
                     LRAGQSAAQIEPLLSALRQHLADTTLAIQATLQCLPVAPAQADHDNPAATPSLLAELA
                     ELVADADSSAQELLQNHSDEFAGVLNESELAQLQQLVDRFDFVRAKALLGTIMDRLGL
                     TREHAYGQHWQEKDCAGG"
BASE COUNT         1812 a         2758 c         2712 g         1802 t
ORIGIN      
        1 gcgggtagtc ggccgtggca atgccgtcct gcgggtaaat cagcaccagt ttttcggcaa
       61 gcgccggatt gcggttcagc gacaacaggg tcgaggcata gttgatgatg ccatcgacct
      121 tgcccggccc tttcaggtag gcctcggcca gccagcccga actgcctgcg gtcaagcgct
      181 gcgccttgaa aaatgccgcc agccgttggg catcaatgtc tttttcttcc agcgcgtcgc
      241 ccttgccaga cagcgcggca gccagaccga gcagcgcaga aaaaccggtg ttcgatgatg
      301 ccgggttggt catgccaaag gtgaactggc cctgggctgc ggcctgcgcg atgtctttcc
      361 aagtgatgtt ggggttgttc ttccagccaa gttcggtcgc tttgcggtct tttagcccca
      421 gcaccaccgg ggtgagcatg gtgcgctcgg aggcgacaat gcgggcccgg gcatccggca
      481 ccagcatcgc gtagcggttg ctggcaagcc aggcggcatc caccgcctcg ccggcctgaa
      541 gcttttcaac cgcttccagg gtgccaacat agcgcatctg cagtgtgatg ccggtggctt
      601 gggtcagcgc gggtaacagt ggctcaatgt ccttgagttc ggagccagcc aatacgctca
      661 aggtgttggc cgggtcgggt ggcgctgcgg cgttatcctg tttgccacag cccgccagca
      721 tcaacgcaaa cagggtcagc aaggtcatca gagttttcat caacacactc ctgccagttt
      781 gaaagggtgg ttcgtcggcg cgtgggtgac gggggcttcg agcatcaaag cttgactcat
      841 ggtagcggct ccttggggca aggctgttta ctggaatttc ttttcaatcg cctgaatcat
      901 gcgctccagg atctcgtaac tcggcgggtc gatcacatcg accaaggtta ccggcgccga
      961 cagcttcctg tccttgagga actcggcaaa aagagcgggc gcggcggtgc ggtagccata
     1021 gtttgcggcc attctttgca gttccgcatc gttggccagc agctcgccga gcttgctacc
     1081 gttttctgtg aaaggtacca gaatgtgctt ggtgtacagc gtgggttgcg ggtagagcaa
     1141 caccatgtcc gggtttggct gggcccgtct ggactggtat tcgaggaatt ggctctcgta
     1201 aatcatcacc agcggtgcct tgccaacgcc catggtggtg tagtcctcaa acgggccggc
     1261 cgagctcgat tcctgcaagc cctggcgcaa gaacagcgag gtcacgaacg gcagcacttt
     1321 gctggcttgc gcctggtcat cgaccacgtt gctgtcgttg accacatagc tggccagggc
     1381 cagatacatg gctgccgagt tggatttgcg cacgtcagtg gaactgatca gaatgctctt
     1441 gctggtggca aacgcggtat tgcccttgag gtcgcgccag cgcacacctt tttcgctcag
     1501 cccaagcagc cgcttcatgt cgatgatgta gtaggtgcca tcggttttct tgacgatgcc
     1561 ttcattttcc atgaccggga tcagcagttg ccaggacgcg attgccatgg gggtaaaaaa
     1621 ggtcggaaac acctgtttcg ctttggcctt gtcttgcagc gtgatggcag caggtgcccc
     1681 ggccggaaaa ccaaagtcgt agcccttgga gtcgcgcgcg gcaatttcgc gagaacccac
     1741 tttttccact ctgacgcgca gccccttggc ggccagccgc tcaatcacct ttgggtcagc
     1801 aaagaaggac tctttttcgg agccaatcat gcctttgaca tcaaccattg ctgccgctgc
     1861 gtctttggct gcctgctctg tcgattggcg accatacgac acataaatgg ccgcgccaat
     1921 gccaatcgcc agaattgcgg caagcgccgg ccccaatacc cgcctgactt ccatagctgt
     1981 tgactccgat caagtgaaac ccatgaactg attgactgcg tgactttaac cctgaaccac
     2041 gtcaaatcag gcgaccgcca attcgatctg aatttatatg cgttttatgc cttcagccct
     2101 cgtggataaa gcgtaactag ctatactaaa aatagcaaat caaggtgctt gcttgcagat
     2161 attcatggcc cggtcagcaa acgcggcaag ccggtggaca gcaccggaat ccatgatatc
     2221 gccagcgtac ccaaaaacat ggccagcacc gccggcagca ccgcgcgcgc cacatagtga
     2281 atgggtttgt caaacatggc cgaggcaaag tagaggttca tgcccgctgg cggggccaaa
     2341 aaacccatct ccatattggc caaaaagatg atgccaaagt gcaccgggtc tatgccgtag
     2401 ctcagcgcca ccggcagcaa cagcggcacc aaaaatacga tggcggcgta gatttccatc
     2461 aaggccccgg cagccagcaa aaatacattg agcgccagca aaaacagata cttgttgggg
     2521 atcatgcctt gcacccagtc gatggcatca ttgggcaagc cgctgtcaac cagatagttg
     2581 gtcagcccca gcgccatgcc caggatcagc atcacaccgc caataatctg cgtgcagtcg
     2641 ctaaatgcgt tgccaagcac ccgccagcct agctcgcggt gcgccagcgc ctgggtggca
     2701 atggcatagg ccgctgtgag tgcagcactc tcggtcggtg tcgccaggcc gctgacaagt
     2761 gagccaatcg ccatcaccgg ggccagaagc tcccacttgg cagcccaaag agtcgcgccg
     2821 atgccggagc caaagtgact gctgaccttg gtgttggccg gtatcaccgc cgctggcatc
     2881 agaccggcac gctggagata gccacccaac agcagcaaaa cacccaccat cacaaaggcc
     2941 ggaatcaccc cggccagaaa cagcgtgttg attggcaccc tggccatgat ggcgtacatg
     3001 atcagcggca ccgagggtgc cagcagtact cccagggcgc tggcactggt gaccagactg
     3061 atgccgcgct gctccggcag gccgacgttg cgaagcagcg gcagcagcag accgcccaaa
     3121 gccagaatgg tcacaccact gccgccggta aacgcagtaa aaaacgaaca cagcaccgct
     3181 gtagccacca ccgtgccgct catgccgcca ccaaacaggg ccacgaacaa agcccccagc
     3241 cgcgctgccg caccagtgcg ggcaaaaacc agccccgcca gcgtgaacaa gggcaaggcc
     3301 ggcagcgatg cgttgacggt gatctggtag tgcgacagcg caaccgaggc cagcggttga
     3361 ccctgttgcc aaaacagcgc cagtgccagg cccccaagca ccgcaaagat cggcgcaccg
     3421 ccgagcaagg ctgctaccaa tatcaacagc atcggccaca agggcagcgg gctgccgtca
     3481 tagatgttgc ccagccaaaa acctgccgct ggcagcacca acgcagccag taacttgatt
     3541 ccgacacggt cagcgcagcg cgcacccagc ttgaacccca gcaagccaaa gcccagcggc
     3601 atcagcagtt gtatccacca tgtgggcatg ccgtaggcca ggttttgagc ggcatctatt
     3661 tcgacagcaa ccagatgcca gctcgccagc gccatcacgc cgcagaccag tgccgcgctg
     3721 ccctgggcaa aagccttgac accaacttgt agccgctccc cgccccaact ggccagacca
     3781 ttgcccaagc tgctcaggtg gccgtggcgc tcggcggcca gtgcacccag catggcaagc
     3841 accaaaccca ggtgctgcac cagcacagcg gcgttttcca caccccggcc catgactgga
     3901 cgcagcatga tctcaagcag cggaatcagc atcatcagcg ccaaagccat tgaggccagc
     3961 gcctcgtcaa agcgctgaag acctttgcag caggaccggc tcagggccgc tgggagcctg
     4021 acgggcttat ggatcatgga ctgcgaaccg agtgcagcgg tccatttttc accggctttt
     4081 tgacccatag ccgggctatg ggcccacaaa tccgccacaa accagcctcg ccggagtcga
     4141 ttctcgccat aagtccggca gtctcctagc ggcccggggt ggcccgaaag gctttgaggt
     4201 gcatgaacac ctcgtcaaag gtgtcggcgg gcaccatgtg gccgcgaatg cgcgggtaga
     4261 gcttgtcggc cagggcactc cactccttca tctgctcggg gttgggtttg ttcaccacca
     4321 gaccacgctg cttcatggcg gccactgcct catcgacctc caggcgggct tgggtgcgca
     4381 tcgccagtcc ggccttttcg ccggcgttgc gcaccacctg ttgtgcactg gggctcatgt
     4441 cgtcccaggc ttttttggtg atcaccaggg caccgacgat cggagcccag ttgatttcca
     4501 gcatgttggg ggcggtgttg tagatctggc tggccagtgc aaagtaaggc gtggacggca
     4561 ccaccgagat catgccggtc tgaatggccg gcaagatgtc gctggtttcc agtggcacag
     4621 gggtgtagcc caggcttttc atgatctctt gctgctcgga ctcggcaccc caggcaaaaa
     4681 atttcatctt caggtaatca gtcggcttga aagccggctc tttggagaaa aaccgcaccc
     4741 agccggagtc accccaggcc agcaccacaa agcccttgtc aaaaaacttc ctttccatgg
     4801 tggcacgcat tttctcgcgc acatagtcca cttcatccca attcttgaac agtagcggca
     4861 ggctttgcaa tgccgtgatc gcgggctcga tctcgcgcag gccgaccacc gaaatcagtg
     4921 cgccttgcaa ttggccaatg cgcatgcgcc gcgccagctc ggcctcaccg ccctggctgc
     4981 catcgggata aatcaggtat ttggcatcgc caccctgggc gctgcgccag gcctcaccca
     5041 ccgcttgcaa ttgccgatgg tagagcgagt ttttcggtgc cagggtgcca atgcgcaatt
     5101 gctgggtggc cgcgtgcgct gcaggaacac tggttgctgc cagggctgcc aggcagatgg
     5161 acagaatttg tcttctcaag gtcggaaaat aagtcatggt gaatgctaaa aaaggtcatc
     5221 tgctgtggcg agcaaccaaa gcgctctctc gcgcatcacg gcgctttgca aatcagcgcg
     5281 cgatgcgctg gccgtcagcg cttgccgcag caaggcatcg aacgcgggcc ggtcccctgc
     5341 cggcaaggcg atgctttcag ctttggccac gaatgcgccc gcacttttgc cagcacccag
     5401 ggtgatggct acatcgaaat accgtgcagc ctgctgccgg gagccgccag ggcgagcggc
     5461 ctcgaaactg cccatcaggg tagccagcgc accttggcca tgctctggtg ctgtctggta
     5521 ggccagacgc gccagtcgaa tcgccagcgg caagtcggcc acgctgttgg gttggtcttt
     5581 ggacaaggcg atcaaaccac cccaggaggc cgccgcccag tacgccagat cgacctcttc
     5641 gggtgatagg tgcaggccat cgatttgggc caaattagcc agtgccttgg caaagcctgg
     5701 gtgacgattt tcgagtgcgg tcatcgcatg ccgatgaccc cgcaggtaca ggcgggcggc
     5761 gcgttcgcgc agtcgttgcg ccgcattcgc atctttggct tcggtacgct cggcttccaa
     5821 ctgcacaaaa gcataggcat actgggtaaa cccagcggcc accgctgccg ccaggggcaa
     5881 gttgcccggt gtttcgcgca gcaccgcttc cgaaagtttg agataaaaag cgctggcctc
     5941 gcgcgccagc acaaggtcgt cttccggcgc attgccttgg gttgccagtg cgctggcagc
     6001 ttcctgaagg atgagttgcc gtggtgaaca agccgtcaga gccaacgaca gcgccaacag
     6061 gacaagatgc agtacggact tcagaggttg ctctcaatca catctgccac gcgctcactg
     6121 tccatcagcg cagtttcagc gtattgcacg ccattgaggc gttgacggta ggccaggtaa
     6181 gcgtgacgat cagcggtgtc ggagttgccc tcctcttcgg catcggcggc tgccaggtgg
     6241 tgacgtgcgg cagcgtcaaa gtgccgggcg gcagcgcgat ggtggtcgcc gaccgactca
     6301 tactcatcac ccgagttgtc ctcggacgag gcgacagggg cgccaaatgt gatttcagcg
     6361 tcttggggca taggggtctc atgagtcgat tgcaggtggt gaagtaccaa ttgctgagtt
     6421 tcgggcaatg acgtgaagga ttcatgacaa gactgttgcc aaaacaaggc atgcaagctg
     6481 ccgcttgcgg tcaacacaag ccccatgcat cgctgcgagg gcactttctc tgatcatcgt
     6541 tttttcgatg gctttgtccg tctgcgctcg cgccacctgc tcagcctgcc acaggcccgg
     6601 gctggggctc tgcgtcacct tcgtcccgaa agctgtgtgc gaccttctgg aactcgtcct
     6661 ggatgccagc aaaccgatca acgatgtcgg ggtcaaaatg ttggccgcgc cccttcatga
     6721 tgatggtcac ggccttgtcg tgcgaataag ccggcttgta cacccgcttg gagatcaacg
     6781 cgtcatacac atcggccagc gccatcaggc gtgccgacac cggaatcgct tcgccaatca
     6841 ggccgtgcgg gtagccgctg ccatcccact tctcatggtg gtgcagcgcg atttcgtgcg
     6901 catagcgcag gaaactgttg ccggtggagc ccatttctcg atctgcccgg gcgatggcta
     6961 cggcaccgat cgaggcatgg gttttcatga tctcgaattc ctcggcatcg aggctgccgg
     7021 gtttgagcag aatgcggtcg ggcacaccga ccttgccgat atcgtgcaga ggggccgagc
     7081 gatacagcga ttcgatcacc tgcggtgtca gcactttgac aaaacgggtg tggcttcgca
     7141 aaccccaggc cagccgttcg atgtaacgct gtgtgcggtt gatgtggttg ccggtttcgt
     7201 tgtcgcgggc ttcggccagc gccgccatgg caccgatggt cacgccctgc accagcgcga
     7261 tctcggcagt gcggtccttg accttttctt ccagcaactg gttctggttt tccagttggt
     7321 cgtgcgcctc tttcaggctc agatgggtgg caacccgggc cttgacgatg gccgggctga
     7381 agggtttgtg gatgtaatcg acggcaccga cgacgaagcc gcgcgcctcg tccatctggt
     7441 cggctagcga ggtcaggaag atgaccggaa tgctgcgact tttcgggtca gtcttgaggc
     7501 gccggcagac ctcgaaacca tccatgccgg gcatctggat gtcgagcaag atgaggtcgg
     7561 gccgccgccg tgcgcccagc accaggtcga gcgcgctggc gccgtcgagt gctgtttgaa
     7621 ccttatagct gtccttgagc acgctgttgg caatggcgat gttttcaggc acatcgtcaa
     7681 ccaccagcac agtctttttc ttgccaatgt tgtccatacg catgttccct ggttaagccc
     7741 aatcgatcca tgattgtgcc taacagcgct ttggcacgga caaaatcaaa tcggtccacc
     7801 aactgctgga gctgggccag ctccgattca ttcaacaccc cagcgaattc gtcgctgtgg
     7861 ttctgcagca attcctgggc actgctgtcg gcatcggcaa caagttcggc caactcggca
     7921 agcaagcttg gtgtcgccgc cgggttgtcg tggtcagctt gcgctggcgc gaccggcagg
     7981 cattgcagcg tggcctgaat ggccagcgtt gtgtcggcca ggtgctggcg cagtgcgctc
     8041 aacagtggtt cgatttgcgc tgcgctctga cccgccctga gtgcgacatc gaccgcagcc
     8101 gcgtcggctg ccagtcggcc cgcacccagg ttggcagcca gacccttgag gctgtgcgcc
     8161 accaaggctg ccacttcacg cctgtcctgc gccagggcat ctgccatttc atcacccata
     8221 ccgtggcggg tctgggcaaa ctggcacagc aggcggtgat acagcacgcg gttgccgctc
     8281 aggcgctgca gaccgtctgc caggtcaatg cctgcaatgg ggggaaggtc ggtgtctgcc
     8341 aggattgtgt tcccgatgtg cccttgctgc tcgggcttga tcggggctgg cctgcggggc
     8401 ggcgtggtgg gcaaccagcg cagtaaggtg gcaaacaggg tttgcggatc aattggcttg
     8461 gacacatgat cgttcatgcc gctggccatg caacgctggc gctcttcttc catcgcgtgg
     8521 gcggtcatgg cgatgatggg cagggcatca tatgccgtgt gagcgcgaat ctgggccgtg
     8581 gcctggtaac cgtccatctc gggcatttgc aagtccatca gcaccagatc aaaagacttg
     8641 tgccgatgca gcacctgatg cactgcttca aggccattgc gggcgattgc cacctcggca
     8701 cctgccgatt caaggatctc gacggcgatc tgctggttga tgtcgttgtc ttcgaccagc
     8761 agcaagcgat agccctgcag cgagggcagt ggcggcgggg gcagacgatg ctggcgcgtg
     8821 tggccggtat ggagctccag cagtgtgtca tgcaacgtcg agggggtgat gggtttggcc
     8881 agtatcgcgt ccatcataga ctgggcaagc tcgccctcaa gaccggcacg gccataggcg
     8941 gtcaccagca ccagcttggg ctggtgctgc agttgcgggt cgtcgcgcac ccgccgggcc
     9001 acatcaaagc catcgagttc gggcatcttc caatcgagaa aaaccacgtc ataggggtcg
     9061 ctggggtcaa cctgccgaac tttg
//
LOCUS       DAOSLL010000119         9041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7286_length_9041_cov_4.515469, whole genome shotgun sequence.
ACCESSION   DAOSLL010000119 DAOSLL010000000
VERSION     DAOSLL010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9041
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7286_length_9041_cov_4.515469"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1491)
                     /locus_tag="PLB25_07055"
     CDS             complement(<1..1491)
                     /locus_tag="PLB25_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOE41372.1"
                     /translation="MQFAEQYATPRHGILRSFFPRVNDFNDLFYLRWGKIDFDVNFGV
                     QVNIKVVLKVYRDNNICETFVVDTEAFDVQWNHHKRCTRDFYIHPTSTQLGQASCVKF
                     SFIVHLDEQSIASQHEYIFMDAQQLQDKNHQSRNISTEWATPNSYRAYQLNAWELQSD
                     VDWYNHHFESLNLVPKFTKGQHNNPYHPKRFIHDHIDKVIASKWQNPDRLCTIKVSVD
                     CIDDHDFINHLIHASQQNVLVQCIVDWRKMTLTNSINYANLKRAHVELIGVVCTPRHH
                     LIEVDPDMHTKFVIFNDEDCILGSFNITFDRWWANWESGMTFHSKGVCRLLDNVFQSQ
                     RGGVIQRYDIEPLSQFNLLYTFGRHFLRNGKHYRPHHAILAEIHRARHSIKLSLFLMG
                     DMLGEHHDSVVDALIQAKDRGVNVHILFNGHLARQGRIGIERTMTEELNRPLLPAVQR
                     LKSAGIRVGLLYGQIDQPVPYSPLHSKYCVIDETTVLDGSFNWYNTS"
     gene            complement(1534..3027)
                     /locus_tag="PLB25_07060"
     CDS             complement(1534..3027)
                     /locus_tag="PLB25_07060"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOE41373.1"
                     /translation="MKKIQLQYVVNETTSKNGLFQQELNFFLLVENLAYEKKIDIVWA
                     GEDEVWQTLPAVYHSSPGLDKEYWQARTALISDEIGKLPGNIQFALSYGVLGEEYWDN
                     NDSLNYHSPAKCGIQMANHHQVLNIGFSTQLEDGQKIIPLTIAVDQALQAQKVTIHWT
                     TDDWRHSNITRCHLPKGNTLDPDGNDDKDRVQVWKGLLIVGHAFRLQYCIRCESADQS
                     LWDNNFGHNYWASRKPLKVLILNLHCYQEKNQDFKFSQIARAIDDLDIDIVCLQEVAE
                     RWNDGEGDWKSNSARIINERLDSPYHLHTDWSHLGFDQFREGVAVLSRYPLEKRDSRY
                     VSNSEDPFNIHARKVVMVQIAVPYIGLLNFFSCHLSWWEDGFEEQFENLREWAASKHT
                     WHVKGTLLCGDFNIRAGSRGYELVVDSNRYTDQFLAVSSPQVYKKIFDDKRPHWQRYL
                     NDDHRIDYIFLKKPSDLKVTSGRVLFTAQDYGTVSDHSGYLMTFEPK"
     gene            complement(3188..4462)
                     /locus_tag="PLB25_07065"
     CDS             complement(3188..4462)
                     /locus_tag="PLB25_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2220 family protein"
                     /protein_id="HOE41374.1"
                     /translation="MKWTTSADLRAQVLKLWDKGDLLRALVSAQPLPPLRLRLSGPVS
                     SDLSERFDSVRTWLDALRGTPRVRIVWREFRHRVLGQSALPHEIWLDTQDDALALAGK
                     QKEAHRFTELLQRSQAHDPALFAALTPWLAKRPLSALALADVWPRLLAVVSWLQRHPR
                     PGIYLRLVDLPGIDSKFIEAHRGVLSELLDLALPTSAVDQDASGINQFCRRYGFRDKP
                     LRLRLRLLDASLALLGSGFDQDITLTQRDFEALAPPVQRVFITENEVNFLAFPPMSAS
                     LVIFGAGYGFDALTGAAWLKRCQLFYWGDIDTHGFAILDQLRAHFPQAQSLLMDQATL
                     LAHAGQWGSEPQPLLRDLPRLSADEAAVFNDLRTNRLRPNLRLEQERIGFGALQSALA
                     SIEPPRQVRMNLAIPLSCALTASTDVALSQAP"
     gene            complement(4482..7961)
                     /locus_tag="PLB25_07070"
     CDS             complement(4482..7961)
                     /locus_tag="PLB25_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE41375.1"
                     /translation="MPGLDFTGTLPLAGFRLMRLEVFNWGTFDKRVWSLELGGKNALL
                     TGDIGSGKSTLVDAITTLLVPSQRIGYNKAAGADARERSLRSYVLGHYKSERNEASGN
                     AKPVALRDARHYSVILGLFHNAGFDKTVTLAQVFWSRDATGQPERFYAVAENALSITA
                     DFSHFGSAIEPLKKRLRKTGATLFDTFPPYGALFRRLFGGLGEQALELFLQTVSMKSV
                     GNLTDFVRHHMLEPFDVGPRIAGLIAHFDDLSRAHAAVLKAKSQLEVLTPLVADCDKH
                     AELLAATDGLRACREALRGHFCRIKLTLLAQLLQVLTEDLARDTAKVNKLAEQRDSQR
                     AQERELRLTLAESGGDRLARIEADIGQRQLEIRRRQANASRYDELRRQLGLLQVASTE
                     DLVAQQQSCLQMQAVETGRADQIQDDLTEKGVELRQARSEHDILMGEITSLKGRRSNI
                     DAQQINMRAELCAALGLDADSMPFVGELIEVRDDQRDWEGAIERLLHNFGLSLLVPDA
                     HYARVAQWVDQTRLKGRLVYFRVRLSLRAEVPSLHPDSLVRKVSVKPDSPFYAWLDRE
                     LCHRFDVACCATQEQFRRETRAITRAGQIKAAGERHEKDDRHRLDDRSRYVLGWSNAA
                     KLAALEAKRRLLEARLAQIGRHIADGQREQSGITERLASLAKLVEYRDYRELDWGLLA
                     REVAALDDERRQLESASDVLQTLTQRLKSLEAAQLGTERQLSEQRDKRARLEQRMADT
                     QARQKQTLELLQAAQGPLAGVNQAQGKATVAAPEPAAAAKSAQSGGEDLLSQAIARHL
                     AELQTRILGTRAITLESCDSREHDMRDWLQKTIDADDIKAKRLVEKIIDAMRSFRHRF
                     PLATQEMDVSVAAAGEFRALLKQLSSDDLPRFEAKFKELLNQNTINQVAQFSAQLNKE
                     RETIKDRISRINQSLTQIDYNPGRFIVLEAQVTTDPEVRDFQTGLRHCLDGAVSGVAQ
                     GEDEQYSETKFLQVKAIIERLRGREGQGEQDRRWTQKVTDVRNWFVFAASERWREDGR
                     EHEHYSDSGGKSGGQKEKLAYTILAASLAYQFGLVWGEARSRAFRFVVIDEAFGRGSD
                     ESAQYGLKLFERLNLQLLIVTPLQKIHIIEPFVSNVGFVHNQEGRDSKLRNLSIEAYR
                     AQKDQMDSAADAT"
     gene            complement(8197..8793)
                     /locus_tag="PLB25_07075"
     CDS             complement(8197..8793)
                     /locus_tag="PLB25_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012431127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4194 domain-containing protein"
                     /protein_id="HOE41376.1"
                     /translation="MTSLVIALLKSVLYRDDDAALWEALLNLQARVRDYVAVLALDLV
                     LDEAEGYAFLRARPQSDDDDAPKLPRLVPRRPLSFQLSLLLALLRKKLAEFDASGGDT
                     RLILSREAVAELVRVFMPAGSNESRLMDLVETQLNKIVELGFLRKLKSSGNLAQSPSY
                     EVRRILKAFVDAQWLGEFDERLASYQAHLGSSTASGDE"
BASE COUNT         1868 a         2666 c         2549 g         1958 t
ORIGIN      
        1 cgaggtgttg taccagttga agctgccgtc aagcacagtg gtttcgtcaa tcacgcagta
       61 tttggagtgc agcggcgaat acggcaccgg ctgatcgatc tgcccgtaca gcagaccgac
      121 ccgaataccg gccgacttga gccgttgcac ggccggcagc agcgggcgat tgagttcctc
      181 ggtcatggta cgttcgatgc caatgcgccc ctgccgcgcc aggtgaccat tgaacaggat
      241 atgaacattg acaccccggt ccttggcctg aatcagggca tcaaccacgc tgtcgtgatg
      301 ttcacccagc atgtccccca tcaggaacag cgacagcttg atcgaatgcc gggcacggtg
      361 aatttcggcc agaatggcgt ggtgcggccg gtaatgcttg ccgttgcgca agaagtggcg
      421 gccaaaggta taaagcaggt tgaattgact cagtggctca atgtcatagc gctggatgac
      481 accgccgcgc tggctttgaa agacgttgtc gagcagccga cagaccccct tggaatggaa
      541 cgtcatgccg gattcccagt tcgcccacca gcggtcaaag gtaatgttga acgagcccag
      601 gatgcagtcc tcatcattga agatgacgaa cttggtatgc atgtccgggt ccacctcgat
      661 cagatgatgc ctgggcgtgc agaccacccc gatcaactcc acatgagcgc gcttcaggtt
      721 ggcgtagttg atgctgttgg tcagcgtcat cttgcgccag tccacaatgc attgcaccag
      781 cacattttgc tggctggcgt gaatcagatg gttgatgaag tcgtggtcgt caatacagtc
      841 cacgctgacc ttgatggtgc aaagccggtc agggttctgc cacttgctgg cgatcacctt
      901 gtcaatgtga tcgtgaatga agcgcttcgg atggtagggg ttgttgtgct gccccttggt
      961 aaatttgggc accaggttga gcgactcaaa atggtggttg taccaatcaa catcagactg
     1021 caattcccag gcattgagtt gataggcgcg ataactgttt ggcgtggccc attcggtact
     1081 gatattgcgc gactggtgat ttttgtcctg caattgctgt gcgtccatga aaatgtactc
     1141 atgctgggac gcaatcgatt gttcatcgag atgcacgata aaggaaaact tgacgcaact
     1201 ggcctggcct agttgcgtcg aagtgggatg aatataaaag tccctggtgc aacgtttgtg
     1261 atggttccat tgcacatcaa atgcttcggt atcaacaaca aaggtttcgc atatattgtt
     1321 gtcgcgataa accttgagca caaccttgat attgacctga acgccgaaat tgacatcaaa
     1381 atctattttt ccccaacgca aataaaaaag atcgttaaag tcgttgaccc tgggaaagaa
     1441 actacgcaga attccatggc gcggtgtggc atactgttct gcaaactgca taaatcacct
     1501 ctcgtgtttt ccaaatattg tttttaatag agctcacttt ggctcgaacg tcatcaaata
     1561 accgctgtga tccgacactg tgccataatc ctgcgcggta aacagaaccc tgcccgaagt
     1621 tactttcaag tcacttggtt tcttcaggaa aatatagtca atgcgatgat catcgtttaa
     1681 atatctttgc caatgaggcc gcttgtcgtc aaagattttt ttatagactt ggggcgagct
     1741 gactgccagg aattgatccg tatatcggtt cgagtcaacc accagttcat accctctgga
     1801 ccctgcccgg atattaaaat caccgcacaa caaggtacct ttgacatgcc atgtatgttt
     1861 ggaggcggcc cattctcgca gattttcaaa ctgctcctcg aagccatcct cccaccagct
     1921 caaatgacat gaaaaaaagt tcagcaatcc aatatagggc acggcaatct gcaccatgac
     1981 gacttttctg gcgtggatgt tgaacgggtc ctcactgttc gagacgtatc tggaatcgcg
     2041 cttttccaat gggtatctgc tcagcacggc aaccccctcg cggaactgat cgaaacccag
     2101 gtgtgaccag tcagtatgaa gatgataggg tgagtcaagg cgctcattga tgatgcgggc
     2161 agaattcgac ttccagtcgc cttcgccatc gttccagcgc tccgccacct cctgcaggca
     2221 gacaatatca atatccagat cgtcaattgc cctggctatt tgagaaaatt tgaaatcctg
     2281 atttttctcc tgatagcaat ggagattgag aatcagcact ttgaggggct tgcggctcgc
     2341 ccaataattg tggccaaaat tgttgtccca taacgactga tctgcagatt cacagcggat
     2401 gcagtattgc aatctaaagg catgcccaac aatgagcaat cctttccaga cttgtacgcg
     2461 atccttatcg tcgttcccat cggggtccaa ggtattgcct tttgggaggt ggcaacgggt
     2521 gatattggaa tgccgccagt catctgtggt ccagtgtatg gttaccttct gggcctgcag
     2581 cgcctgatcc acggcaatcg tcagcggaat gattttctgc ccatcttcca gttgcgtgga
     2641 gaagccgata ttcagcacct gatgatgatt cgccatctga atgccgcatt ttgccgggct
     2701 gtggtagttc aggctgtcgt tattatccca atattcttcc cccagaaccc cataagataa
     2761 ggcaaactga atgtttccag gtaattttcc gatctcatcc gaaatgagtg cagtcctggc
     2821 ttgccagtat tccttgtcca aaccaggcga gctgtggtag accgcaggca aagtttgcca
     2881 tacctcatct tcaccagccc agacaatatc tatctttttc tcgtaggcaa gattttcgac
     2941 cagtaaaaag aaattcaatt cctgctggaa aagcccgttc ttgcttgttg tttcattgac
     3001 gacatattgc agttgaattt ttttcatcca gacactccaa agttttatta ttcatccctg
     3061 ccatgccttg gcctgccaac cgtaatgtct ctgccgctgg ccgatattca tgttacctca
     3121 acgaaccagc gctcaatcag gtaggcgggc ggcgttgcag gcagaccatc ggtaccaagt
     3181 ggcggcatca gggggcctgg ctcaatgcca cgtccgtgct cgcggtcaaa gcacaggaca
     3241 aggggatagc aagattcatg cgcacctgcc gtggcggctc aatggatgcc aaggcgcttt
     3301 gcagtgcgcc aaaaccgata cgttcttgct ccagccgcag gtttggcctt aaccggttgg
     3361 tgcgcaggtc gttgaacaca gcggcctcat ctgcgctcag gcgcggcaaa tcgcgcagca
     3421 ggggttgcgg ctcgctgccc cactgcccgg cgtgcgccag caaggtggcc tggtccatca
     3481 gcagcgactg cgcctgtgga aaatgggcgc gcaactggtc cagaatggca aaaccgtggg
     3541 tgtcgatgtc gccccagtaa aacagttggc agcgcttcag ccatgcggca ccagtcagcg
     3601 cgtcaaaacc gtaacccgcg ccaaaaatca ccaggctggc tgacatcggc ggaaaagcca
     3661 gaaagttgac ttcgttttcg gtgataaaca cccgctgcac cggcggtgcc agcgcctcaa
     3721 agtcccgctg ggtcagcgtg atgtcctggt caaaaccgct gcccaacaat gccagactgg
     3781 cgtcaagcaa gcgaaggcgc aagcgcagcg gcttgtccct gaaaccgtag cggcgacaaa
     3841 actggttgat gccgctggca tcctggtcaa ctgccgaagt cggcaaagcc aggtccagca
     3901 attcgctcag aacaccccgg tgggcctcaa tgaacttgct gtcgatgccg ggcaaatcga
     3961 ccaagcgcag gtagatgcct gggcgcggat ggcgctgcag ccagctcacc acggccagca
     4021 agcgcggcca gacatcggcc agcgccaggg cgctcaaggg acgcttggcc agccaggggg
     4081 tgagcgccgc aaacaatgcc gggtcatgtg cctggctgcg ctgcagcagc tcggtgaacc
     4141 gatgcgcctc tttttgcttc ccagccaagg ccagcgcatc gtcttgcgtg tcgagccaaa
     4201 tctcatgcgg cagcgcgctt tggccgagca cgcggtgcct gaattcgcgc cagacgatgc
     4261 gcacccgtgg cgtgccgcgt agcgcatcaa gccaggtgcg cacgctgtca aaacgctcgc
     4321 tcaggtcgga cgagaccggg ccactcaggc gcaggcgcag cggcggcagc ggttgcgccg
     4381 acaccagcgc tcgcaacagg tcacccttgt cccacagctt gagcacctgg gcgcgcaggt
     4441 cggctgaggt ggtccacttc atgatttgcg ccgaatccgg gtcaggtcgc atcggcagcg
     4501 ctgtccatct ggtctttttg agcccggtac gcctcgatgc tgaggttgcg cagcttcgag
     4561 tcgcggccct cttggttgtg gacaaaaccg acgttcgaca caaagggctc gatgatgtgg
     4621 attttttgca gcggggtaac aatcagcagt tgcaggttga gccgctcaaa caatttcaac
     4681 ccatattgcg ccgactcgtc agagcctcgg ccaaaagcct cgtcaatcac gacaaaacgg
     4741 aaagcacgtg agcgtgcctc gccccacacc agaccaaact gataggccag actggctgcc
     4801 agaatggtgt atgccagctt ttccttttgc ccaccagact tgccgcctga gtcggaataa
     4861 tgctcatgct cgcggccatc ttcgcgccag cgctcactgg ctgcaaaaac aaaccagttg
     4921 cgcacatcgg tcactttttg cgtccagcgc cggtcctgct cgccctgccc ttcgcggcca
     4981 cgcagacgtt caatgatggc tttcacctgc aaaaacttgg tttcggaata ctgctcatct
     5041 tcaccctggg cgacaccgga tacggccccg tccagacaat ggcgcaggcc ggtctgaaag
     5101 tcacgcactt ccgggtcggt cgtcacctgg gcttcaagca cgataaaacg acccgggttg
     5161 tagtcaattt gtgtcagcga ctggttgatg cggctgatcc ggtccttgat ggtttcacgc
     5221 tctttgttga gctgggcgct gaactgagcc acctggttga tggtgttctg attgagcagt
     5281 tccttgaact tggcctcaaa gcgcggcagg tcatcgcttg agagttgttt gagcaacgcc
     5341 cgaaattcac cggcagccgc cactgacaca tccatttcct gggtcgccaa agggaaccgg
     5401 tgacgaaagc tgcgcatcgc atcgatgatt ttttccacca ggcgttttgc cttgatgtca
     5461 tcggcatcaa tggttttttg caaccagtcg cgcatgtcgt gctcacggct gtcacacgac
     5521 tccagcgtga tcgccctggt gcccagaatg cgtgtttgca gctcggccaa gtgccgggca
     5581 atggcttgcg acagcagatc ttccccgccg ctctgggcgc tctttgctgc ggcagccggt
     5641 tcgggggcgg caaccgttgc tttcccctgc gcctggttaa cgcctgccag tgggccttgg
     5701 gccgcttgca gcagctccag cgtctgcttt tgccgcgcct gggtgtcagc cattcgctgc
     5761 tcaagcctgg cgcgcttgtc acgctgctcg gacagttgtc tctcggtccc caactgcgcc
     5821 gcttccagtg acttcaggcg ctgcgtcaac gtttgcagca catccgatgc cgactccagt
     5881 tgtcggcgtt catcgtcaag cgcggccact tcacgcgcca gcaggcccca gtcgagttcg
     5941 cggtaatcgc ggtattccac cagcttggcc aggctggcca gccgctcggt gatgcccgac
     6001 tgctcacgct gcccatcggc gatgtgtcgg ccaatctgcg ccaggcgcgc ctccagcagc
     6061 cggcgcttgg cctccagtgc cgccagcttg gcggcattgc tccagcccag cacataacgg
     6121 ctgcggtcat caagccggtg ccggtcatcc ttttcatggc gctcaccggc tgccttgatc
     6181 tggccggcgc gggtgatggc ccgggtctca cgcctgaact gctcctgcgt ggcgcagcag
     6241 gccacatcaa aacggtggca cagctcgcgg tcgagccagg cataaaacgg cgaatcaggt
     6301 ttgaccgaca ccttgcgcac caggctgtcc gggtgcaggg aaggcacttc agcgcgcagg
     6361 ctcaggcgca cccgaaaata caccaaccgc cccttgagcc gggtttggtc cacccactgc
     6421 gccacccggg cgtaatgcgc atcgggcacc agcagcgaca ggccaaagtt atgcagcagg
     6481 cgctcaatgg caccttccca gtcgcgctgg tcatcgcgca cctcgatcag ctcgccgaca
     6541 aacggcatgc tgtcggcatc aagccccaat gctgcacaca gttcggcgcg catgttgatt
     6601 tgctgcgcgt cgatgttgct gcgccgccct ttcaggctgg tgatctcgcc catcaggatg
     6661 tcatgctcgc tgcgagcctg cctgagttcc acgccctttt cggtcaggtc gtcttgaatc
     6721 tggtcggcgc gcccggtctc aaccgcctgc atttgcagac agctttgctg ttgtgccacc
     6781 aggtcctcag tgctggccac ctgcagcagg cccaactggc ggcgcagttc gtcatagcgg
     6841 ctggcattgg cctgacggcg acggatctca agctgacgtt gcccgatatc ggcctcgatg
     6901 cgggccagcc ggtcgccgcc gctttcagcc agggtcaggc gcagctcgcg ctcctgggcg
     6961 cgctggctgt cgcgctgctc tgcgagcttg ttgaccttgg cggtatcgcg tgcaagatct
     7021 tccgtcaaca cctgcagcag ttgcgccagc agcgtcagtt tgatgcggca aaagtggcct
     7081 cgcaaggcct cgcgacaggc gcgcaagcca tcggtggcgg ccagcagttc ggcgtgtttg
     7141 tcgcaatctg ccaccagcgg cgtcagcact tcaagctggc ttttggcctt gagcacggcc
     7201 gcatgggcgc ggctgaggtc gtcaaaatgg gcgatcaaac cggcgatgcg cgggcccacg
     7261 tcaaatggtt cgagcatgtg gtggcgcaca aagtcggtca ggttgcccac cgatttcatc
     7321 gacacagtct gcaaaaacaa ttccaatgcc tgttccccca ggccaccaaa caggcggcga
     7381 aacaacgcgc cgtagggtgg aaaagtgtca aacagggtgg caccggtttt gcgcaggcgc
     7441 tttttcaaag gctcgatggc gctgccaaag tggctgaagt cggcggtgat ggacaaggcg
     7501 ttttctgcca ccgcataaaa acgctcgggc tggccagtcg catcgcggct ccagaacacc
     7561 tgcgccagcg tcacggtttt gtcgaacccg gcgttgtgaa acagccccag aatcaccgaa
     7621 tagtgccgcg catcacgtag cgccaccggt ttggcattgc cactggcctc gttgcgctcg
     7681 gatttgtaat gccccagcac ataggagcgc agcgagcgct cccgcgcgtc agccccggcc
     7741 gccttgttgt agccaatgcg ttgcgacggc accagcagcg tggtgatggc atcgaccagg
     7801 gtcgatttgc ccgaaccgat gtcgccggtg agcagcgcgt ttttgccgcc cagctccagc
     7861 gaccagaccc gcttgtcaaa ggtgccccaa ttgaagactt cgaggcgcat caggcgaaaa
     7921 ccggccagcg gcaaggtgcc ggtgaaatcg agtccgggca tgtcggactg aacggactga
     7981 acggactgaa cggactggtc ggactggtcg gactggtcgg acattttgat gggcttggtc
     8041 actgggttgt atctttgtca ttgccgcgcc ggtggcagtc catgagcgcc ggattcccgc
     8101 ctgtgcagaa attgcgattt gaggtcggga tggccacctg agcgatacgc aggatgctaa
     8161 cgctgttgcc gcccagccaa ggtctagccc aagggcttat tcatccccgg atgctgttga
     8221 actgcccaga tgggcctgat aactggccag ccgctcatcg aactcgccca gccattgcgc
     8281 gtccacaaaa gccttcaaaa tccgccgcac ctcatagctg ggcgactgcg ccaggttgcc
     8341 gctgctcttg agtttgcgca gaaagccgag ttcgacgatc ttgttgagct gagtctccac
     8401 caagtccatc aggcgcgact cgttgctgcc agcaggcatg aacacccgca ccaattcggc
     8461 aaccgcctca cgcgacaaga tcagacgggt gtcgccgccg ctggcatcaa actcggccag
     8521 ctttttgcgc agcagcgcca gcagcaagct gagctgaaaa ctcaagggcc gccgtggcac
     8581 cagacgcggc aatttgggcg cgtcgtcatc atcagactgg ggccgggcgc gcaaaaaagc
     8641 atagccctcg gcctcgtcga gcaccagatc gagcgccagc accgccacat agtcgcgcac
     8701 gcgggcttgc agattcagca atgcctccca caaagccgca tcgtcatcgc gataaagcac
     8761 gcttttgagc agcgcaatca ccaggctggt caggtcatgg ctggcttgac tggcttgact
     8821 ggcttgactg gcttgactgg cgctgacctg actggcactg gcctgactgg cgctagcatg
     8881 actggcctga ctggcctgac tggcttgact ggcctgactg gcttgactgg cataactggc
     8941 gctggcataa ctggcgctgg cctgactggc gctagcctga ctggcattgg cactggcact
     9001 ggcactggcc tgactggcct gactggggct ggcttgactg g
//
LOCUS       DAOSLL010000120         9030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7300_length_9030_cov_4.698942, whole genome shotgun sequence.
ACCESSION   DAOSLL010000120 DAOSLL010000000
VERSION     DAOSLL010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9030
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7300_length_9030_cov_4.698942"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..940)
                     /locus_tag="PLB25_07080"
     CDS             complement(17..940)
                     /locus_tag="PLB25_07080"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOE41377.1"
                     /translation="MNLTLPTAAHQSQPVRHMATPETPVESLLVLPATEDLLKSIGMP
                     SRPSTLVQLQDEIASEDPDSRRIADLVASDVALTVAALRIVNSPVFALSRRCETVEQA
                     ISMLGLQQLNVIVTGLTLRNLMRGDARQLREFWDTSSKRAYALSRLARGLGLVEVHVA
                     QSFGLFCDIGIPLLLQRFADYEQTLIICHGESERCFTAPEQDRHHTDHAQIGAMMARS
                     WGLSPTLCLAIGLHHDYSVFVNPKVPETVARLVAMNLLAEAAIQRFAGQASSTEWGKG
                     GDHASGALVLNGQDTEDWIERLFDDFAKGLG"
     gene            1134..1703
                     /locus_tag="PLB25_07085"
     CDS             1134..1703
                     /locus_tag="PLB25_07085"
                     /inference="COORDINATES: protein motif:HMM:NF017496.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOE41378.1"
                     /translation="MGLPQAIHQMSAQAFTAWELDQPERHEFFRGEVLRVFGMGGARR
                     EHVRATMNIDRSLDNHLTGTPCQAFMADMKVHVEATGDMFYPEILVTCDPRDLRASLE
                     MQHAKLIIEVLSPTTATFDRGDKFLTYRRIEALQEYALADPHAKTFEVYRRQANLSDW
                     LLTQGHPEEGLVLHSVALTLPSGATFENV"
     gene            complement(1869..2222)
                     /locus_tag="PLB25_07090"
     CDS             complement(1869..2222)
                     /locus_tag="PLB25_07090"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOE41379.1"
                     /translation="MAEVLELATRGQAKCHGCFITLMEVFYRVWRDEDKAAGQLAYQQ
                     CLALPIEWLSNTESLLAKAAEYKAMYPLSIADAWIAACASELGAVLLHKDPQFKPLPV
                     AQEWLPLKVKKTQIG"
     gene            complement(2503..3135)
                     /locus_tag="PLB25_07095"
     CDS             complement(2503..3135)
                     /locus_tag="PLB25_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE41380.1"
                     /translation="MIRLLIADDHAILRAGLRQLFALAPDMQVVCEAASGAEVLACLR
                     QSQIDLLLLDLNMPGISGADLIARVKAHQCDLPILVFSMHNEPLVATRVLKAGASGYI
                     TKDCEPDILLAAVRKVAAHGNYLDPGIAEKMAFDATSSAARPLHTLLSDRELEVFRLL
                     TSGQSVNAIATQLAISNKTVSTHKTRLLEKLNLSGMAELMRYAMQHDLLG"
     gene            complement(3132..3485)
                     /locus_tag="PLB25_07100"
     CDS             complement(3132..3485)
                     /locus_tag="PLB25_07100"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE41381.1"
                     /translation="MCELQVPEDAPVLDANRAVVLFRIVQESLNNIGKYAQASQVVIS
                     LARRGQDLWLELRDNGQGFDMAAVAQRGTLGLLGMRERVLALGGRVEINSAPGQGTVI
                     TVVIALDADVTRDLA"
     gene            complement(3507..5531)
                     /locus_tag="PLB25_07105"
     CDS             complement(3507..5531)
                     /locus_tag="PLB25_07105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE41382.1"
                     /translation="MQAAPAQAVTPDLLQRAQILAQQEAAASSTGDVADLSPQALREA
                     LQNLRLHQFELEMQNDELRRTQAALDTAKARYFDFYDLAPVGYVTVSDQALILQTNLT
                     AAKLLGLPRAELVGKALPGFLLAPDADSFYLLCKKALTSGNAQSTELRMRRPDSQPFW
                     AQLHAIAATGDDGAAVIRLVLSDITARKQLEERLISSEADTKALLDGASDAIFINDPQ
                     GRFQYVNQRAVQLLGYSRGELLGMCVTDITPAEDFQLTRRMLAQLMLTGSLRYEPSLL
                     CRDGSTVPAELTGALLSNGNGFAACRDITERRQFERAAKEASEYKFRLVADNTSDGIV
                     IFGADRHIKYVSPAYVKQLGYSEAEERNRSPEQIYALIHPEERDALFARLARALSSKE
                     DDLRYSYRIQHQLGHYIWREDSARFQYDESGSFSGACVVTRDITLRRQAEVDLRIAAA
                     AFETQEAMMITDANSVILRVNRAFTQVTGYTAEEAVGRTPRLLQSGHHDKDFYRQLWE
                     TVRSTGNWQGEVWDRHKDGQVYPKWLAISSVTGIDGVVSHYIGSHFDLSERKRAEAAM
                     QEMNRNLKRSRQQLRRLVALNETTLEKEKRHIAREVHDELGQVLTALRMDLSLAIIRH
                     ASEVPDLLDALKRMKIQVDRAILGVRNVATSLRPAALDLGLVPAIEWLCH"
     gene            complement(5690..7489)
                     /locus_tag="PLB25_07110"
     CDS             complement(5690..7489)
                     /locus_tag="PLB25_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE41383.1"
                     /translation="MGWIYGGLSLVLCSCLLFYQQRRRVFQRLAHVNESALRASDARL
                     RSFFEATPDALLISDAHGSITMANRQVESLLGYSMADLLGKSIEALVPVRFRDKHLTM
                     RDGFVAAPDTRQMGKGVTARALRKDGSECDVEISLSRIETDDGIFFASALRDITERLQ
                     ADEKIRELAFYDQLTGLPNRTLLSDRLKQAISASNRSGICSALVLIDLDYFKTLNDTL
                     GHDIGDLQLKQVATRLSTCVRECDTVARVGGDEFVVILAGLGSSEVEAATDTKAVVAK
                     ILAALNQPYQLGEVFHSSSASIGITLFQDQRVSMDDLMKQADLAMYRAKDAGRNAMRF
                     FDPSMEAVVVKRVAMEKDLRHALNSNQFMLHYQAQVEGAGRVTGAEVLVRWLHPERCM
                     VPPNDFIALAEETGVILALGHWVLETACSQLEQWGQLPGLAHLTLAVNVSALQFAQAD
                     FVEQVMTVVQRTGANPLHLKLELTESLLVGNISDIIEKMVRLKSQGIGFSLDDFGTGY
                     SSLSYLSRLPLDQLKIDRSFVSDALTNPDDAAIARTIIALAHSLRLGVIAEGVETDAQ
                     REFLAGAGCHAYQGYFFGRPLPVAEFEAFVRSH"
     gene            complement(7865..8386)
                     /locus_tag="PLB25_07115"
     CDS             complement(7865..8386)
                     /locus_tag="PLB25_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41384.1"
                     /translation="MSDRTVHTGTRRSLAEWLLCAVLLLSWGGYLGYSKHQDYDQIAF
                     SEQTRLSNQALVIEKNLTSQLLSANRVLEGLLVELASVPSSKAAASTFNERLKLISDT
                     LPGVSPILIINASGKIVHSSVASLLGFDASGRDYFQTALKRRRQNTLYVSAPFTSALG
                     STAISLVREITGA"
     gene            complement(8492..>9030)
                     /locus_tag="PLB25_07120"
     CDS             complement(8492..>9030)
                     /locus_tag="PLB25_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE41385.1"
                     /translation="VTMRGINEASGKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVASEVRALAGRSAEAAKEIKTLINASVVRVAQGTSQVDQAGTTMQEVVASIK
                     RVTDIMGEISAASSEQSLGVSQVGAAVTEMDQVTQQNAALVEQMAAAASSLMSQANQL
                     VETVAVFKLQPDGLGEIA"
BASE COUNT         1812 a         2758 c         2627 g         1833 t
ORIGIN      
        1 ctgccactcg gcgaaatcag ccaagccctt tggcaaagtc atcaaacagg cgctcgatcc
       61 agtcttcggt gtcctgcccg ttgagcacca gggcaccgct ggcatggtcg ccgcctttgc
      121 cccattcggt gctgctggct tgaccagcaa agcgctggat cgccgcttca gccagcaaat
      181 tcatcgccac caggcgcgcc actgtttccg gcactttggg gttaacgaac accgagtagt
      241 catggtgcag cccaatggcc agacacaagg tgggcgacaa accccaactg cgcgccatca
      301 tggccccgat ctgggcgtga tcggtatggt ggcggtcttg ttccggtgcg gtgaaacaac
      361 gctcgctctc accgtgacag atgatcagcg tttgctcata gtcggcaaag cgctgcagca
      421 acagcgggat gccaatgtca caaaacaggc caaacgactg cgccacatgc acctccacca
      481 gcccgagtcc ccgagccagc cgggacaggg cgtaggcgcg cttgctcgag gtgtcccaaa
      541 attcgcgcaa ctggcgggca tcgccgcgca tcaaattgcg caatgtcagg ccggtcacga
      601 tgacgttcaa ctgctgcagc cccagcatcg agatcgcctg ctctaccgtt tcgcagcgac
      661 gcgacaaggc aaaaaccggg gaattgacaa tccgcagggc cgcaacggtc aatgccacat
      721 cgctggccac caggtcggca atgcgccgac tgtccgggtc ttcgctggcg atctcgtcct
      781 gcagttggac cagggtgctg ggccgcgacg gcatgccaat gctcttgagc aagtcctcgg
      841 tggctggcag cacaagcagg ctctcgaccg gcgtttcagg tgtcgccata tggcgcacgg
      901 gctggctttg gtgggctgca gtgggcaagg tcaggttcat cagggttggc ctcggtgcat
      961 tggattgagc gtgggtcagg tacatctgtc catcgtgccc aagccggccg cccggcgcca
     1021 tagggcaatg ctgatttggc tatagggcga tgctgagcgc gggtcagcat tgccctaccc
     1081 gttgcggcta caggtgaaac tggccgaccg gctgcagtgc aatcatttgg gccatgggtt
     1141 tacctcaagc cattcaccaa atgagcgcgc aagcgttcac tgcctgggaa ctcgaccagc
     1201 ctgaacgcca tgagttcttt cgcggtgaag tgttgcgggt ctttggcatg ggcggcgcgc
     1261 gacgcgagca tgtccgcgcc accatgaata tcgaccgctc gctggacaac catttgaccg
     1321 gcacaccttg ccaggcattc atggccgaca tgaaggttca cgttgaagcc accggcgaca
     1381 tgttttaccc cgaaattctg gtcacctgcg atccgcgtga cctgcgtgca tcgctggaga
     1441 tgcagcacgc caagctgatc atcgaagtgc tgtccccaac gacagccacc tttgatcgcg
     1501 gcgacaagtt cctgacctac cggcgcatcg aggccttgca ggaatatgcg ctggcggacc
     1561 cgcacgccaa aaccttcgag gtctatcgcc gtcaggccaa tttgagcgac tggttgctga
     1621 cgcaaggcca ccctgaagaa ggtctggtgc tgcacagtgt cgccctgacg ctgccatcgg
     1681 gcgcaacgtt cgaaaacgtc tagtgctggc ctgatgcagc gctttggggt ttcaccgggc
     1741 tggtccaaaa cagcttctgc acttttgaac gatttagccc tccagccccc gtggaataag
     1801 cgctaccagc tatatattca atagcaaatc aaagtgttcc cgatgtttcg gctgaccgcc
     1861 gcgcgacttc agcctatttg ggtcttcttg actttgagtg gcagccattc ctgtgcaact
     1921 ggcaggggct tgaattgcgg atccttatgc agcaggacgg cacctagctc tgacgcacac
     1981 gcggcaatcc aggcatctgc gatggacagc ggatacatgg ctttgtattc ggcggctttt
     2041 gccagcaagc tttccgtgtt tgacagccac tcgatcggca aagcgaggca ttgttggtag
     2101 gccagttgac ccgccgcctt gtcctcgtct cgccacacgc ggtagaacac ctccatcagg
     2161 gtgataaagc atccgtggca tttcgcctga cctctggtgg caagctccag cacttcggcc
     2221 acccggtcgg caccagctca atcgggtgcg tagcgctgtc acccaatggg tgaatcccat
     2281 cgaaacgaaa aaagtaaaca atcataaaaa cttacagatg aaaacaagcg gtcaactgaa
     2341 tctaggttca atagtcctgg cggtgatctg tccggcctgc gccgcgttgt ctcaagttat
     2401 aaggcattca gccctccagc acccgtggaa tatgcgctgc cagctatcca atcaatagta
     2461 attcgaagaa ttcccaatgc tccggctggg catcgcgcaa gttcaaccca gcaagtcatg
     2521 ctgcatcgcg tagcgcatca actcggccat gccagagagg ttgagcttct ccagcagccg
     2581 cgtcttgtgg gtgctgacgg ttttgttgct gatggccagt tgggtggcga tggcattgac
     2641 actttggccg ctggtcagca ggcgaaacac ttcaagctcg cggtccgaca gcagggtatg
     2701 cagcgggcgc gctgccgagg atgtggcatc aaacgccatt ttttcggcaa tgcccggatc
     2761 caggtagttg ccatgtgcag ccaccttgcg caccgctgcg agcaaaatgt cgggctcgca
     2821 gtctttggtg atatagccgc tggccccggc cttgagcacg cgcgttgcca ccagcggctc
     2881 gttgtgcatg ctaaatacca gaatgggcag gtcgcattgg tgcgccttga cccgcgcgat
     2941 caaatccgcg ccgctgatgc cgggcatgtt caggtccagc agcagcaggt caatctggct
     3001 ctggcgcagg cacgccagca cctcggcacc gcttgcggcc tcgcagacta cctgcatatc
     3061 gggggccagc gcaaaaagct gccgcaatcc agcccgcaag atggcatggt catcggcaat
     3121 cagcagccgg atcatgccaa gtccctggtc acatcggcat ccagcgcaat caccacggta
     3181 atcaccgtgc cttgtccagg tgcgctgttt atttccaccc gcccgcccag ggccagcacc
     3241 cgctcacgca tgcccagcaa acccagtgtg ccgcgctggg ccaccgctgc catatcaaag
     3301 ccctgcccgt tgtcgcgtag ctccagccac aggtcctggc cgcgacgcgc caagctgatg
     3361 accacctggc tggcttgggc gtatttgccg atgttgttga gcgactcctg cacgatgcga
     3421 aacagcacca ccgcacggtt ggcatcaagc accggcgcat cttcgggtac ctgcagctca
     3481 cacatgacac caccgtgccg ggtaaattaa tggcagagcc actcaatggc gggcaccagc
     3541 cccaggtcca gcgccgccgg tcgcagactg gtggcgacat tgcgcacgcc caaaatcgcc
     3601 cggtcaacct gaatcttcat gcgcttgagt gcatcaagca ggtctggcac ctcgctggca
     3661 tggcggatga tggccagcga caggtccatg cgcagtgcgg tcaagacctg gccgagttcg
     3721 tcatgcactt cacgggcaat gtggcgtttt tccttttcca gcgtggtctc attgagcgcc
     3781 accaggcggc gcaactgctg gcgcgaccgc ttaaggttgc ggttcatttc ctgcatggca
     3841 gcctcggcgc gcttgcgctc gctcaagtcg aagtgggagc caatgtaatg actgacaaca
     3901 ccgtcgatgc cggtgaccga cgaaatggcc agccacttcg gataaacctg gccgtccttg
     3961 tggcggtccc agacttcgcc ttgccagttg cctgtgctgc gcacggtttc ccacaactgg
     4021 cgataaaagt ctttgtcgtg gtgccccgat tgcagcaagc gcggggtccg acccacggct
     4081 tcttcagccg tgtagccggt gacctgggtg aaagcccggt tgacccgcag gatcaccgag
     4141 ttggcatcgg tgatcatcat ggcttcctgc gtctcgaatg ccgctgctgc aatgcgcagg
     4201 tcaacttccg cctggcggcg caaggtgatg tcgcgggtga cgacgcaggc tcctgaaaaa
     4261 ctgccggatt cgtcgtactg aaacctggcg ctatcttcgc gccagatgta gtgccccagt
     4321 tgatgctgga tgcggtagga atagcgcaga tcgtcctctt ttgacgagag cgcacgcgcc
     4381 aggcgggcaa acagggcatc gcgctcttct ggatgaatca gggcgtagat ctgctccggt
     4441 gagcgattgc gttcttcagc ctcgctgtag cccagttgct tcacataggc tggcgaaaca
     4501 tatttgatgt gtctgtctgc gccaaaaatc acaatgccgt cggaggtgtt gtcagccacc
     4561 agcctgaact tgtattcgct ggcctctttg gccgctcgtt cgaactggcg acgttcggtg
     4621 atgtcgcggc aggcagcaaa gccgttgcca tttgaaagca gcgcgccagt caactctgcc
     4681 ggtacggtgc tgccgtcccg gcataaaagc gatggttcat agcgcagcga gccggtcagc
     4741 atcaattggg caagcatgcg tcgggtcaac tgaaaatctt cggctggggt gatgtccgtg
     4801 acgcacatgc ccagcaattc acctcggctg taacccagca gttgcacggc ccgctggttg
     4861 acgtactgga agcgcccctg cgggtcgttg atgaagatgg catctgatgc gccgtccagc
     4921 agcgctttgg tatcggcttc gctactgatc aggcgctctt caagctgttt gcgtgccgtg
     4981 atgtcgctca acaccaggcg gatcaccgct gcgccgtcgt cgccagtggc ggcaatggcg
     5041 tgcaactgtg cccaaaacgg ctggctgtcg ggcctgcgca tgcgcagctc ggtgctttgg
     5101 gcattgccgc tggtcagggc ctttttgcac agcaggtaaa agctgtcggc atcgggggcc
     5161 aagagaaagc cgggcagcgc tttgccgacc agctcggcac ggggcaggcc cagcagcttt
     5221 gccgcagtga ggttggtttg caggatcagc gcctggtcgc tgacagtgac ataacccacc
     5281 ggggccaggt cgtaaaagtc gaaataacgt gcctttgcgg tatcgagcgc ggcctgcgtg
     5341 cggcgcaact cgtcgttttg catctccagc tcaaactggt gcaggcgcag gttttgcagc
     5401 gcttcgcgaa gcgcctgcgg cgacaggtca gcgacatcac cagttgagct tgcagcggct
     5461 tcctgctgcg ccaagatttg agcccgctgc aacaggtcag gggtcaccgc ttgggccggg
     5521 gcagcttgca cagggggcgt atcggaccca tgagtgcctc tgaggggcgt cactattggc
     5581 tgcacatggt ggcctgggtt gacgaatgag cttgcttgaa gcgcaaggcg aaacgggcag
     5641 tgtagtcctt gcctgccagg cgggccgatt tccattggcc tgatgcccgc taatggctgc
     5701 ggacaaacgc ctcaaattcc gccaccggca gcggccgacc aaagaaatag ccctgataag
     5761 cgtggcaacc cgcgcccgcc agaaattccc gctgcgcatc ggtttcaaca ccttcggcaa
     5821 taactccaag gcgcaaactg tgcgccaacg cgatgatggt tctggcaatg gccgcatcgt
     5881 ccgggttggt caacgcatcg ctgacaaaag agcggtcaat cttcaactgg tccagcggca
     5941 ggcggctcag gtaagacagc gacgaatagc cggtgccgaa atcatccagt gagaaaccaa
     6001 tcccctgact tttcagacgg accatctttt cgatgatgtc cgagatattg cccaccagca
     6061 ggctttcggt cagctccagc ttcaggtgca gcgggttggc accagtgcgc tgcacaacgg
     6121 tcatcacctg ttcgacaaaa tcagcctggg caaattgcag tgcgctgaca ttcaccgcca
     6181 gcgtgagatg cgccaagccg ggcagttgcc cccattgttc caactggctg caggccgtct
     6241 ccagcaccca atgccccagc gccaagatca cgccggtttc ttccgccagc gcaataaagt
     6301 cgtttggcgg caccatgcag cgctcaggat gcagccagcg caccagtact tcggcaccgg
     6361 tcacgcggcc agcaccctcc acctgcgcct ggtagtgcag catgaactga ttggagttaa
     6421 gcgcatggcg caagtctttt tccatggcaa cacgtttgac caccaccgcc tccatgctcg
     6481 gatcaaagaa gcgcatggcg ttgcggcctg cgtccttggc ccggtacatg gccagatcag
     6541 cctgtttcat cagatcatcc atgctgacgc gctggtcctg aaacagcgtg atgccaatgc
     6601 tggcactgct gctgtgaaac acctcgccca actggtaggg ctgattgagt gcggccagaa
     6661 tttttgccac cacggctttg gtgtcggtag ccgcctcgac ctcgctgctg cccaggccag
     6721 ccagaatcac cacaaactcg tcacccccga cgcgggccac cgtatcacac tcgcgcacgc
     6781 aggtgctcaa gcgcgtggcc acctgtttga gctgcaagtc gccaatgtca tggcccagcg
     6841 tgtcattgag ggttttgaag tagtccagat caatcaacac caacgcgctg caaatgccac
     6901 tgcggttgct ggcggaaatg gcttgtttca aacggtctga cagcaaggtc cggttaggca
     6961 aaccggtgag ctggtcatag aaagccagct cgcggatctt ttcgtctgcc tggagccgct
     7021 cggtgatgtc gcgcagggcg ctggcaaaaa agatgccatc gtcggtctcg atccgactca
     7081 gactgatctc cacgtcgcat tcgctgccgt ccttgcgcag cgccctggct gtcacccctt
     7141 tacccatttg ccgggtgtct ggtgccgcca caaaaccgtc gcgcatggtc agatgtttgt
     7201 cacgaaaacg cactggcacc agtgcttcaa tcgacttgcc cagcagatcc gccatcgaat
     7261 agcccagcaa agactcgact tgccggttgg ccatggtgat gctgccgtgg gcatcgctga
     7321 tcagcagggc atcgggcgtg gcctcaaaaa acgagcgcaa acgcgcatcg ctggcgcgca
     7381 gggcgctttc attgacgtgc gccaggcgct ggaaaacacg gcgacgctgc tggtagaaca
     7441 gcaggcacga acagagcacc aggctcaagc cgccatagat ccagcccatc tccgtcattt
     7501 cccggcgcca gttggcataa atggctgaca tatcacgcgt aacgctcacc accagaggct
     7561 tgtccatgcc gggctgggat ggctgcacgg tgtgaaaggc catcatcttc gcagcccccg
     7621 ggtggcaagc tgtgccgacg aacacactga cagcctggcc actgctcttg tgctgatcaa
     7681 aaaaacagcc tggccgggcc tggtcgtggt cgaccagatc agtcagcgct ggtacgctca
     7741 tgaaaagctt gccgtcgccg tgaaccagag cgatgtgggt gtcgggtgta aagcgcactg
     7801 aatcgagcag cacgccaaaa aagatcgggt caagcgcggc cagcaccaca ccgccgaact
     7861 cgccctaggc accggtgatt tctcgcacca gggaaatggc ggtagaaccc aacgcgctgg
     7921 taaaaggcgc cgagacgtaa agtgtgtttt gcctgcgccg cttgagcgcc gtttgaaagt
     7981 agtcccgccc ggacgcgtca aaccccagca ggctcgccac acttgaatga acgattttgc
     8041 cgctggcgtt gatgatcagg atgggggaga caccgggcaa cgtgtcagat atcagtttca
     8101 gccgttcatt gaatgtgctc gctgctgcct tgcttgatgg cacgctggcc aattcgacca
     8161 gcaacccctc cagcacccgg ttggctgaaa gcaactggga ggtgaggttt ttttcaatca
     8221 ccagcgcctg gtttgagagt cgggtttgtt cgctgaacgc gatctggtca tagtcctgat
     8281 gcttcgaata gccgagatag ccgccccagc tcaacaaaag taccgcgcac agcagccact
     8341 ctgccaggct gcgacgcgtg ccggtgtgaa cagttctatc ggacatttcg aggtaatgtg
     8401 gggctgcagc aaatacccgc tgtcagcgtg cggcgagaat tgattgacca actataagcg
     8461 gtcttgcatt gacgtttcta ctttggagcg gttaagcaat ttcacccaag ccatcgggct
     8521 ggagcttgaa caccgccacc gtctcgacca actggttggc ctgcgacatc aggctgctgg
     8581 ccgctgctgc catttgctcc accagcgccg cgttctgctg ggtcacctgg tccatttccg
     8641 tcaccgctgc accaacctgg ctgacaccca gactttgttc gctgctggca gcactgatct
     8701 cacccatgat gtcagtgaca cgcttgatgg aggccaccac ttcctgcatg gtggtgccgg
     8761 cttgatcgac ctggctggtg ccctgtgcca cccgcaccac actggcgttg atcagggtct
     8821 tgatttcttt ggcggcttca gcgctgcgac ccgccagggc acgcacttca ctggccacca
     8881 cggcaaagcc acgtccctgt tcaccggcgc gggcggcttc cacggcagcg tttaaggcca
     8941 ggatgttggt ttgaaaggca atgccgtcaa tcacgctgat gatgtcggca atcttgcctg
     9001 aggcttcgtt gatgccgcgc atggtgacca
//
LOCUS       DAOSLL010000121         9027 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7301_length_9027_cov_4.265381, whole genome shotgun sequence.
ACCESSION   DAOSLL010000121 DAOSLL010000000
VERSION     DAOSLL010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 9027)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9027)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9027
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7301_length_9027_cov_4.265381"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(107..1339)
                     /locus_tag="PLB25_07125"
     CDS             complement(107..1339)
                     /locus_tag="PLB25_07125"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HOE41386.1"
                     /translation="MIRRIAHLDMDAFYASVELLRYPQLKGLPVVIGGGRRPADDAFL
                     TMGETGSLAKIPLEVFPRLVSYTGRGVITTATYAARQFGIGSAMGLMKAAKLCPQAIL
                     LPVDFAEYRRYSRAFKDIILDIAPLMEDRGVDEVYIDFTEVPGGQREGGRVLARLIQK
                     TIFQQTGLTCSVGVAPNKLLAKMASEFNKPNGIAIVFEEDLQSKIWPLACRKINGIGP
                     KAEEKLQALGIRSIGELAQKEVDWLIEAFGQKTGAWLFDASHGLDPRAVVLESEPVSI
                     SRETTFERDLHAVHDKLELGAIFTQLCTQVAQDLNARGYIGKTIGIKLRYDNFKSVTR
                     DQTLNFYTDDAKVIRQTAGQCLKRVPLQQRLRLLGVRVASLAAADDPPQYMKNRPVAP
                     ADSALEAIDLEAVERQLF"
     gene            1464..4151
                     /locus_tag="PLB25_07130"
     CDS             1464..4151
                     /locus_tag="PLB25_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HOE41387.1"
                     /translation="MDKHFLSSLFSPGSIAIFAGKPDDPESQTSQARALHQAITAQHF
                     AGKLVFLDVNASGTLADLANAHADLAIIALPAAEVAAALEIAGRIKCHSALVISSGID
                     AAHATELHRIATRDGIYLLGPNCLGFQRPHMLLNASVAGEMANPGPLALVSQSGALTS
                     SILDWSRKNAVGFSTVVSLGPNTAVDMAQVLDFLASDVRTHSIVVYLEGITNARRFMS
                     ALRAAANVKPVVVLKAGRKHGGNRAALTHSGTIVGSDDVFDAALRRAGAVRVRSFVAL
                     FSAAKCLASRYRPVGRRLAIVTNGGGPGVLAADWITEIQLDLGRLMPEQALQLKPLLP
                     SQATLSDLIDLSEEAGPEHYRAAIDAASKASQIDGILAIYSPKVGMDGDAVATAIAAM
                     SRKASKPLLTCWMGDASVGSSRKLLTDAVIPTFRTPEAAVGAFGNLASFYQNQQLLQQ
                     TPPPLSDLSLPDVEGARLLIESVLAERRKVLTEMESKALLAAFHIPVTKTILARNPNE
                     AIMIATQLGFPVALKIDSPDISHKSDVQGVALNIMSAVGVRDTFLEMMQNVARLQPDA
                     RINGITIQNMSNQKRGREVCVGLVTDQPFGPVIAFGAGGTMIELINDRTMELPPLNQF
                     LARRLIERSRVFETLGQWRGAPAADIEALEQILLRVSEMVCELPQLREMDINPIIVDE
                     SGALAVDARIVIDNAAPSVRHYNHLAILPYPSHYQQICPMAGGGDYLLRPVHPDDATM
                     LQAFVRGLSPESRYFRFVSSMQELPATMLSRFTLIDYDREMALVAIHTESGLGEDGQS
                     QDISRIVGVSRYITNPDRSTCEFSLVVADEFKGLGLGSRLMLSIMDFAREKGLAEIEG
                     LVLANNPNMLKLMKGLGFKIKSFPEDPDFKLVTHMLQPS"
     gene            complement(4165..5313)
                     /locus_tag="PLB25_07135"
     CDS             complement(4165..5313)
                     /locus_tag="PLB25_07135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HOE41388.1"
                     /translation="MPVITNIEDLRVLAKQRVPRMFYDYVDSGSWTESTYRANSEDFQ
                     KILLRQRVAINIDHRSTSSSMIGQAVAMPVALAPTGLTGMLCADGEIKAAQACKNFGV
                     PFTLSTMSICSIEDVAKETGGHPFWFQLYVMKDRDYIERLIERARAANCAALMLTLDL
                     QISGQRHKDLRNGLSAPPKLTLPNMLNMATKLRWGLGMLGTRRRGFGNIVGHVKGVDN
                     MDNLSEWTSRQFDPTLCWNDVAWIKKRWGGKLILKGIQDVEDARLAVSSGADALVVSN
                     HGGRQLDGAASSIRALPTIVDAVGSQIEVHMDGGIRSGQDVLKARALGARGTYIGRAF
                     LYGLGAMGEAGVTKALQIIHKELDLTMAFCGHTRIDKVDKSILLTNPV"
     gene            5474..6613
                     /locus_tag="PLB25_07140"
     CDS             5474..6613
                     /locus_tag="PLB25_07140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOE41389.1"
                     /translation="MITTDVLIIGAGMAGASAAYFLAPQRRLVLLEREAQPGYHATGR
                     SAALYSETYGNATVRAITSASRAFYFAPPKGFADHPLVAPRGALTFGSAADHAALLDT
                     WQSMRALVPNVQWWTQAELLQRVPVLRPELAECGFFEPDAMDLDVHAIHQGFLRGAKG
                     AGAQLLCDAGVHQIRREAGGWSVATAVGNFFAPLLINAAGAWCDELAQLAGVATIGLV
                     PKRRTALTCDAPAGVNISNWPLVIDAQESFYFKPDAGVLLISPANEDPVAPQDVQPEE
                     LDIAIAVDRIETATTLQIRQVRRKWAGLRSFVADKSPVVGFAPDAPGFFWLAGQGGYG
                     IQTAPAMGQLAAALVQGQPVPEALAALGLRYQDVSPARAALGSEK"
     gene            complement(6657..6905)
                     /locus_tag="PLB25_07145"
     CDS             complement(6657..6905)
                     /locus_tag="PLB25_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41390.1"
                     /translation="MDQLKALESAGLVLPSPAYILGAIIFGLIGLIAFRRGRKATQPT
                     LTWVGLALMLYPYVVPQTWLLWAIGAALSAWVFASWKA"
     gene            complement(6936..7649)
                     /locus_tag="PLB25_07150"
     CDS             complement(6936..7649)
                     /locus_tag="PLB25_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE41391.1"
                     /translation="MGAQWKAKHKDLAANAKGRVFGKLSKDIMLAARSGPDPASNARL
                     RLVVEQARKVSMPKETLDRAIKKGAGLTGESVNFEHVIFEGFAPHRVPVMVECLTDNT
                     KRTAPEMRVLFRKGQLGTSGSVAWDFDHLGMIEAEPAVSGADPELAAIEAGAQDFEPG
                     QEDGTTLFLTDPADLDLVSRALPVQGYSVLSAKLGFKAKNPVDPASLSAGQLEEVETF
                     LAAIDANDDVQNVYAGLAG"
     gene            complement(8013..>9027)
                     /locus_tag="PLB25_07155"
     CDS             complement(8013..>9027)
                     /locus_tag="PLB25_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE41392.1"
                     /translation="QRMGGPGGGGGAPPFKEADAEAIAAQVGGLMAVAPEARSAASIV
                     ADGRNWSSSVIGSTNNWLITGNWKLAEGRNFSDEEQHGGAAVCLIGNTVRRELFGSGS
                     PLGASIRVKQIACEVVGLLDSKGQGAFGNDQDDIVLTPLHTLQRRITGNTRVNTLLVS
                     MQDGADPERVKAALTDLLRERRKLAQDDEDNFNVLDTKQLGETFASTTRVMTMLLGAV
                     AAVSLLVGGIGIMNIMLVSVTERTREIGLRLAIGALEREVLMQFLIEAVVLSALGGVI
                     GIVLAFGASIGVARLMDVPFVFDAGVNLLSFVFSAAIGVLFGYFPARRAARLDPIEAL
                     RHE"
BASE COUNT         1708 a         2706 c         2731 g         1882 t
ORIGIN      
        1 ttttagcggc tcacaattct taaagccccc ggctttgccg ggggatggtt acttggtgaa
       61 aaagtgcact gccttgcgtg cttgaacctg tgcaactgaa ctttcgtcaa aacagttgcc
      121 gctcaactgc ttccaaatca atagcttcca gcgcactgtc ggcgggggct acaggccgat
      181 ttttcatata ttgtggcgga tcatcggcgg cggccagcga agccacccgc acgcccagca
      241 agcgcaggcg ctgttgcaag ggcacgcgtt tcaggcattg cccggcggtc tggcggatga
      301 cttttgcatc gtcggtgtaa aaattcagcg tctggtcgcg cgtcacgctt ttgaagttgt
      361 cgtagcgcag cttgatgcca atggtcttgc cgatatagcc ccgggcgttc aggtcttgcg
      421 ccacctgggt gcacagttgg gtaaagatgg cacccaattc gagcttgtcg tgtaccgcgt
      481 gcaggtcgcg ctcaaaggtg gtctcccggc tgatggacac tggttcgctt tccagcacca
      541 ccgctcgcgg gtcaagtcca tgggacgcat caaacagcca ggcaccggtt ttctggccaa
      601 acgcttcaat cagccagtcc acctcttttt gcgccagttc gccaatgctg cgaatgccca
      661 gcgcttgcag cttttcttcc gccttggggc caatgccgtt gattttgcgg caggccagcg
      721 gccagatttt gctttgcaaa tcctcttcaa acacaatcgc gatgccgttg ggtttgttga
      781 actcgctggc cattttggcc agcagcttgt tgggtgcgac accgaccgag caggtcaggc
      841 cggtttgctg aaatatggtt ttctgaatca gccgtgccag caccctgccg ccttcgcgct
      901 ggccccccgg tacctcggta aagtcgatgt agacctcatc gacgccgcgg tcttccatca
      961 gtggcgcaat gtcaaggatg atgtctttga aggcgcgcga gtaacgccgg tactcggcaa
     1021 agtccactgg cagcaggatc gcctgcgggc aaagtttggc ggctttcatc agccccatgg
     1081 ccgagccgat gccgaactgc cgggccgcgt aagtcgccgt tgtaatgaca ccacgaccgg
     1141 tataactgac cagccgggga aacacttcaa gcgggatttt ggccaaagac cctgtttcac
     1201 ccatggtcag aaatgcgtcg tccgccgggc gccgaccacc gccaatcacc accggcaaac
     1261 ctttgagttg cgggtagcgc agcaactcta ccgaggcgta aaacgcgtcc atatccaggt
     1321 gggcgatgcg gcgaatcatg gggggagagg atagcgcggc agaaaaaatg ctgcagtgca
     1381 caatttagtg aacaaagtcc ggtaatttgc ctgactaaac ggttaagctc tacacaacgg
     1441 cactttagtc aataggtagt ttcatggata agcatttttt gagctcgctt ttttcacccg
     1501 gctccattgc gatttttgcc ggaaagcctg acgatcctga atcacaaact tcacaggcgc
     1561 gggccttgca ccaggccatt accgcccagc actttgccgg caaactggtt tttcttgacg
     1621 tgaacgccag cggcacgctg gccgatctgg ccaacgccca cgctgacctt gccatcattg
     1681 ccctgccagc ggctgaagtg gcggcagcac ttgagattgc cgggcgcatc aagtgccact
     1741 cggccctggt gatttccagt ggtatcgatg ccgcacatgc cactgaattg cacagaatcg
     1801 ccacccgcga tggcatttat ctgctcgggc ccaactgcct gggctttcag cggccgcaca
     1861 tgctgctcaa cgccagcgtg gcgggcgaaa tggccaaccc cggcccgctg gcgctggtgt
     1921 cgcagtccgg tgccttgact tcatcgattc tggactggtc cagaaaaaac gcggttggtt
     1981 tttcgacggt ggtgtcgctt ggtcccaata ccgccgttga catggcgcaa gtgctcgact
     2041 ttttggcctc cgatgtccgc acccacagca ttgtggtgta tctcgaaggc attaccaatg
     2101 cgcggcgctt catgagtgcc ctgcgcgcag ccgccaatgt caagccagtg gtggtgctca
     2161 aggccgggcg caaacacggc ggcaaccgtg ctgccctgac gcattcaggc accattgtgg
     2221 gcagcgacga cgtgtttgac gcggccttgc gccgggccgg tgcggttcgg gtgcgctcgt
     2281 ttgtggcctt gttttcagcg gccaagtgtc tggcatcgcg ttaccgtccg gtgggccgac
     2341 ggctggcgat tgtcaccaac ggtggtggcc ctggtgtgct ggcagcagac tggatcaccg
     2401 aaattcagct tgatctggga cgtctgatgc ctgagcaggc gctgcaactc aaaccgttgc
     2461 tgccgtcaca ggcgaccctg agcgacctga ttgatctgtc cgaagaggct ggccccgagc
     2521 actatcgcgc cgccatcgat gcggccagca aggcatcaca gattgatggc attctggcca
     2581 tttattcgcc caaggtcggc atggatggtg atgcggttgc caccgcgatt gccgccatga
     2641 gccgcaaggc cagcaagccg ctgctgacct gctggatggg tgatgcctcg gtgggcagtt
     2701 cgcgcaagct gctgactgac gcggtgatcc cgacctttcg cacgcccgag gctgccgtcg
     2761 gcgcgtttgg caacctggcc tccttctatc agaaccagca gttgttgcaa caaacgccgc
     2821 caccgctgtc agacttgtcc ctgcccgacg tggaaggggc gcgcctgttg atcgaaagtg
     2881 tgctggccga gcggcgcaaa gtgctgaccg agatggagtc caaggccttg ctggcagcct
     2941 ttcatattcc ggtcaccaag accattctgg ctcgcaaccc caatgaagcc atcatgattg
     3001 ccacgcaact gggctttccg gtggcactca agatcgattc gcccgacatc agccataaat
     3061 ccgatgtgca gggtgtggcg ctcaatatca tgagtgcagt gggtgtgcgt gacacttttc
     3121 tggaaatgat gcaaaacgtg gccaggctgc agcccgatgc gcgcatcaac ggcatcacca
     3181 tccagaacat gtcgaatcaa aagcgtggcc gtgaggtctg cgtcggtctg gtcaccgacc
     3241 agccctttgg cccggtgatt gcgtttggtg ctggtggcac catgatcgag ctgatcaacg
     3301 accgcaccat ggaattgccg cccttgaatc aatttttggc gcgccgcctg attgagcgtt
     3361 cacgcgtgtt tgaaacgctg ggccagtggc gtggtgcacc ggccgccgat attgaggcgc
     3421 ttgagcagat tttgctgcgg gtgtctgaaa tggtgtgtga actgccgcaa ctgcgcgaga
     3481 tggacatcaa cccgatcatc gttgacgaat ccggtgcgct ggccgtcgat gcccgcattg
     3541 tgattgacaa cgccgcgcct tcggtacggc attacaacca cctggccatc ctgccgtatc
     3601 cgtcgcacta ccagcagatc tgtccaatgg cggggggcgg cgactacctg ttgcgcccgg
     3661 tacaccctga cgatgccacc atgctgcagg cttttgtgcg cggtctgtcg cccgagagcc
     3721 gttatttccg ctttgtctca agcatgcagg agttgcctgc caccatgttg tcgcgtttca
     3781 cgctgatcga ctatgaccgc gagatggcgc tggttgccat ccataccgag agtggtttgg
     3841 gcgaagacgg ccagtcgcaa gacatcagcc gaatcgttgg cgtgtcgcgt tacatcacca
     3901 atccggaccg cagcacctgc gagttctcgc tggtggtggc cgatgaattc aagggcctcg
     3961 ggctgggctc gcgcctgatg ctgtcgatca tggactttgc ccgcgaaaaa gggctggctg
     4021 aaatagaagg tctggtgctg gcaaacaacc ccaacatgct caagctgatg aaggggctgg
     4081 gcttcaagat caagtcgttc ccggaagacc ccgacttcaa gctggtgacc cacatgctgc
     4141 agccatcctg agcggctggg gatttcagac ggggttggtc agcaaaatgc ttttgtcaac
     4201 cttgtcaatc cgggtgtgtc cacaaaacgc catggtcagg tcgagttcct tgtgaatgat
     4261 ctgcagggct ttggttacac cggcttcgcc catggccccg agtccgtata aaaaggcccg
     4321 gccaatgtag gtgccacggg caccgagtgc gcgcgctttg agcacgtctt gcccgctgcg
     4381 gatgccgccg tccatgtgca cctcgatctg gctgccaacg gcatcgacga ttgtcggcag
     4441 tgcccggatt gatgacgctg cgccatcaag ctggcggccg ccatggttgc tgacgaccag
     4501 cgcatcagcg cctgagctga ccgccagcct ggcatcttcg acatcctgaa tgcctttgag
     4561 gatgagcttg ccaccccagc gttttttgat ccaggccacg tcattccagc acagcgtcgg
     4621 gtcaaattgg cgggaggtcc attcgctcag gttgtccatg ttgtccacgc ccttgacgtg
     4681 gccaacaata ttgccaaagc cgcgccgccg ggtgccaagc atgcccaggc cccagcgcag
     4741 cttggtcgcc atgttgagca tgtttggcaa ggtcaatttg ggtggggccg acaggccgtt
     4801 gcgcaaatcc ttgtggcgct ggccgctgat ctgcaagtcc agggtcagca tcagcgccgc
     4861 gcagttggcc gccctggcac gctcgatcag gcgctcgatg tagtcgcggt ctttcatcac
     4921 ataaagctga aaccagaacg ggtgaccacc ggtttccttg gccacgtctt caatcgagca
     4981 aatgctcatg gtcgaaagcg tgaagggaac accaaaattt ttgcaggcct gggccgcctt
     5041 gatttcgccg tcggcacaca gcatgccggt aagcccggtg ggtgccagcg ccaccggcat
     5101 ggccacagct tggccaatca tgctgctgct ggtgctgcgg tggtcgatgt tgatggcaac
     5161 ccgctgacgc agcagtattt tctgaaaatc ttccgaattg gcgcggtagg tgctctcggt
     5221 ccagcttccg gagtcaacgt agtcgtaaaa catgcgcgga acgcgttgtt tggccagaac
     5281 gcgaaggtct tcaatgttgg tgatcacggg catgcctgtt tccttggggt tgtgctgatg
     5341 ttacgttttc aaaacggcca tctgcagtga aaaatcggat cggccgggtt gaaatttgaa
     5401 gccgcagcac ctgacaatca tgagccggtg ggctgcggtg cctggccgac actggtttcg
     5461 agtgggctca atcatgatca caacagacgt tctcatcatc ggcgcaggca tggctggcgc
     5521 atctgctgcc tattttctgg cgccacagcg gcggctggtg ctgctcgagc gcgaggcaca
     5581 gcccggttat cacgccaccg gtcgctcggc tgcgctttac agtgaaacct atggcaacgc
     5641 gaccgtgcgg gccatcacca gcgcgtccag ggcgttctat ttcgcgccac caaagggttt
     5701 tgccgatcac ccgctggtgg caccgcgcgg cgcgctgacc tttggcagtg cggctgacca
     5761 tgcagcgctg ctggatacct ggcaaagcat gcgtgcgctg gtacccaacg tgcagtggtg
     5821 gacccaggcc gagttattgc aacgcgtgcc ggtgctgcgc cccgagcttg ccgaatgcgg
     5881 attttttgag cccgatgcga tggacctgga cgtgcatgcg attcaccagg gttttctgcg
     5941 cggcgccaaa ggcgctggcg cgcaactgct gtgtgatgcg ggtgtgcacc agatccgccg
     6001 cgaggcaggt ggctggtcgg tggccacggc agtcggcaat ttctttgcgc cactgctgat
     6061 caatgccgcc ggtgcctggt gtgatgaact ggcgcaactg gccggcgtgg caaccattgg
     6121 cctggtgccc aagcggcgca ccgcgttgac ttgcgatgcc ccggcggggg tcaacatcag
     6181 caactggccg ctggtgattg acgcgcagga gagcttttac ttcaagcctg atgcgggcgt
     6241 gttgctgatc tcgcccgcca acgaagaccc ggtagccccg caggatgtgc agccagaaga
     6301 actcgacatc gccattgcgg tggaccggat cgagaccgcc accacgctgc aaatccggca
     6361 ggtgcgacgc aaatgggcgg gcttgcgctc atttgtcgcc gacaagtcgc cggtggtcgg
     6421 gtttgcgcca gatgcaccgg gtttcttttg gctggcaggg cagggcggtt acggcattca
     6481 gactgcgccg gccatggggc agcttgcggc agcgctggtg cagggccagc cggtgcctga
     6541 agcgctggcg gcacttggcc tgcgctatca agatgtgtcg ccagcgcgcg cagcgctcgg
     6601 gtcagaaaag tagcggcaat gcgtttgccg ccggactggc cacaggctgg ccaatgtcac
     6661 gccttccaac tggcaaagac ccaggcgctc agtgctgcac caatagccca gagcagccag
     6721 gtttgcggca cgacataggg gtacagcatc agcgccaggc cgacccaggt gagcgttggc
     6781 tgagttgcct tgcgcccgcg tcgaaacgcg atcaggccaa tcagcccgaa gatgatcgcg
     6841 cccagaatgt aggcaggcga gggcagcacc agaccggccg attcaagcgc tttgagttga
     6901 tccatgtgac agtgtcgcgt gcgccgatcg gggtgttaac cggccaggcc agcatagaca
     6961 ttctgcacat cgtcattggc atcaatggca gccagaaagg tttccacttc ttcgagttgc
     7021 ccggcgctca ggctggccgg atcaaccgga tttttggcct tgaagccaag tttggcagac
     7081 agcacgctgt agccctggac cggcagtgcg cgactgacca ggtcaaggtc ggcagggtcg
     7141 gtcaaaaaca gcgttgtgcc gtcttcctgg cccggctcaa agtcttgcgc gccagcctcg
     7201 atggcggcca gctcggggtc ggcaccgctt accgcaggct cggcctcgat catgccaaga
     7261 tggtcaaagt cccacgccac cgagccggaa gtgcccaact gacccttgcg aaacagcacc
     7321 cgcatttcag gcgcggtgcg cttggtgttg tcggtcaggc attcgaccat cactggcacc
     7381 cggtgcggtg caaaaccctc aaaaatcaca tgttcaaagt tgaccgactc gccagtcaaa
     7441 cctgccccct ttttgatggc gcggtccagc gtctccttgg gcatcgagac cttgcgtgcc
     7501 tgctccacca ccagccgcaa acgggcgttt gaggcggggt cgggtccgct gcgggcggcc
     7561 agcatgatgt ctttggacaa ttttccgaac acccggccct tggcattggc ggccaggtct
     7621 ttgtgttttg ctttccattg cgcgcccatt tgcttgaatt tccttgttgt ttgcgcccag
     7681 tgcgccgggt tgaccatgat ggaccaagtc tgactttgat acaaaaaaca gcgcttggtc
     7741 gaagcggcaa gggtacacca ggccgggctc aagtcccacc attgacgatg gggtgaatgg
     7801 cgtttgcgac aacttgacct caccgagcaa gccagaaatt ccaaagcgct atagtataga
     7861 tagctgccag cgcatatgcc acaagggcta tggcccattt ctatccaaaa attgaatcag
     7921 gccagaggcg gatcactggc ccagcggctt tgccgggcag ggcgttagcg gtggacgctg
     7981 ggaccgcgag acgcatcagg ccactggcat gcctactcgt gccgcagtgc ctcgatcggg
     8041 tccagccggg cggcccggcg cgccggaaaa tagccaaaca gcacaccaat ggcggccgaa
     8101 aacacaaatg acagcaggtt gacaccggca tcgaagacaa acggcacgtc catcagccgg
     8161 gccacgccga ttgatgcacc aaaggccagc acaatgccga tcacgccacc cagagccgac
     8221 agcaccacgg cttcgatcag aaattgcatc agcacctcgc gctccagcgc gcctattgcc
     8281 aggcgcaagc cgatctcgcg ggtgcgctcg gtgacactga ccagcatgat gttcatgatg
     8341 ccaatgccgc ccaccagcag gcttaccgca gcgaccgcac cgagcagcat ggtcatcacc
     8401 ctggttgtgc tggcaaaggt ctcgccgagt tgtttggtgt cgagcacgtt gaagttatct
     8461 tcgtcgtctt gcgccagttt gcgtcgttcg cgcagcaggt cggtcagcgc agccttgacc
     8521 cgttcggggt cggcgccgtc ctgcatcgac accagcaggg tgttgacgcg ggtgttgccg
     8581 gtgatgcgtc gctgcagtgt gtgcagcggt gtcaggacga tgtcgtcctg gtcgttgcca
     8641 aaggcaccct ggcccttgga gtcaagcagg cccaccacct cgcaggcaat ttgcttgaca
     8701 cgaatcgacg cgcccagcgg cgagccgctg ccaaacagct cacgccggac ggtgttgccg
     8761 atcaggcata cggcggctcc gccgtgttgc tcttcgtcgc tgaaattgcg gccttcagcg
     8821 agtttccagt tgccggtgat cagccagttg ttggtgctgc cgatcacgct gctgctccag
     8881 ttgcgtccgt cggcgacgat gcttgcggca ctgcgcgcct ctggtgccac tgccatcagg
     8941 ccaccaacct gggcggcgat ggcctcggca tcggcttcct tgaagggtgg cgcaccgcca
     9001 ccaccgcctg gcccgcccat gcgttgg
//
LOCUS       DAOSLL010000122         8984 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7339_length_8984_cov_4.695487, whole genome shotgun sequence.
ACCESSION   DAOSLL010000122 DAOSLL010000000
VERSION     DAOSLL010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8984
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7339_length_8984_cov_4.695487"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PLB25_07160"
     CDS             <1..648
                     /locus_tag="PLB25_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE41393.1"
                     /translation="YQVAYYAADMLVLLAQLQPATLDWLGTSMGGLIGIAVAGQLELP
                     AFANIRRLVLNDVGPVIEWQALQRIGQYLGRTGKFDSEQQAADAIWTVSSSFGPHTSA
                     QWLALTRPNLKPLGDGSGRLTLNYDPALALPFATVTQESAAQGEALLWQAYDQIRAST
                     LLVRGADSDLLSEATAQAMTQRGPKARLIEFAGVGHAPTFVAADQVQAILSFLLD"
     gene            733..2982
                     /locus_tag="PLB25_07165"
     CDS             733..2982
                     /locus_tag="PLB25_07165"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003079822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOE41394.1"
                     /translation="MRKSSNDGTVAASASKSVLAVVAVTSSDLSAQANALVRARAFAE
                     PLLVGEIFDTGEGVLAHADAVAAILKGIGGSEAMQAASYLVYVSDHLNKPREVIGKAF
                     GDNFAELALETSKLVQVQRQARRAQLARQAVEPDNAAAQQVAAVQLENVRKMLLAFSR
                     DLRVVMLRLASRLQTLRFFAAAKLPVPQEIATEALQVFAPLANRLGIWEIKWEMEDLA
                     FRFLEPDTYKQVARLLDEKRVQREAHVERMRVQLEQDLNAHGISARVSGRPKHIYSIV
                     KKMRGKSLGFERVFDVRAMRIVVPSVPECYGALSWVHSHYSPIAEEFDDYIAKPKGNG
                     YQSLHTVVRDASGQPTEIQIRTQAMHDHAENGVAAHWAYKEAGAKGYAGVTASGAYDG
                     KIAVLRQLLAWERDLSGAPSQAHAVLDDRIYVLTPDAAIVELPRGATAVDFAYAVHTS
                     LGHRCRGARVDGAMVPLHTPLKNGQTVEVTTAKEGGPSRDWLNPELGYLVSHRARTKV
                     RAWFNALAMNATIAKGREAVEKILQREGKTALKLDELAVQLGFANAESLFEGVGKDEF
                     SLRNIEQLLRPPESPQFDADAPLLRKARSGDNPTKGGLLVLGVDSLMTQMAKCCKPAP
                     PDQICGFVTRGRGVSVHRTDCTNLREMVAKNGERLIEVEWGVANAVSHSVYPVDVAVQ
                     AQDRQGLLRDISEVFTKEKMNVTGVQTQSIKGIAWMTFTVEVSDSGRLHKVLGLVLEL
                     PGVRWARRK"
     gene            3017..4462
                     /gene="sbcB"
                     /locus_tag="PLB25_07170"
     CDS             3017..4462
                     /gene="sbcB"
                     /locus_tag="PLB25_07170"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-exoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008852 - exodeoxyribonuclease I activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HOE41395.1"
                     /translation="MAQHTFLWHDYETFGANTRRDRPAQFGAIRTDADLNQIGDPIEL
                     YCQPANDFLPDPQACLITGITPQMCLEQGLPEYQFAAQIEQALAWPGTVGVGYNTIRF
                     DDEITRFMFWRNLIDPYAREWQNDCGRWDLLDVVRTAYALRPDGVQWPVDPDGKPSFK
                     LTDLTAANGLAHASAHDALSDVHATLALARLIKQAQPRLFDFCFGLHKKDRVMAELGL
                     PTSLRNALPFWHVSGMFAPGRGCLALMWPLAMHPGNKNELLAWDLAQDPGELVSLNAQ
                     QIRTRLFTRSADLPDGVRRLPIKSVHLNKSPMVMGKLKVLSEAMAQRWGIDLDTQFAH
                     ARKARDLPDMSAIWAQVFKREGDVLLDVDEDLYGGFIGNADRRHLNELRQSTPEQLAK
                     ARPAFDDPRLSELLWRYRARNFPQSLAPEEASRWEAHRASRLFEGAGGARTVQALFDE
                     MDLLAETADERGEAVLGALYDYAEMIAPQLA"
     gene            complement(4742..5296)
                     /locus_tag="PLB25_07175"
     CDS             complement(4742..5296)
                     /locus_tag="PLB25_07175"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HOE41396.1"
                     /translation="MKFTLCATALAVLVGCANTTPPRPEPTYEDAAASKFVVSSREAI
                     NKLVAGFDLGPLGDTPVLVATIVNVNDTRRSAPLGRTLSEQYASHMVNAGFNVKEVKL
                     RGDVFVKEETGELLLSREIKEIAQSHHANYVLVGTYSAAATVTYVSLKFVRTGDGRIV
                     RGHDYALPNDKDITKLLQVVPARQ"
     gene            complement(5299..5955)
                     /locus_tag="PLB25_07180"
     CDS             complement(5299..5955)
                     /locus_tag="PLB25_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41397.1"
                     /translation="MRLLAICAGAALMTACATSPIPVSENFPLTVQPKVRSAGHWDIL
                     SKDVIVQTLDTLDKVSAPAPLYVTLPPQPSAFDRAFREFLITELVQAGRIVVQYPDNA
                     LEVSYQTQVVRHASPRPHFVPGIFTMLTTGLYAAYGLRLEHADAKLLGGLGLAVLADT
                     VASVDSGGPTHTELILTTTIAAGGRFLTRNTDVYYIEEEDQFLFATLPVHRTVKVVGP
                     "
     gene            complement(6146..6931)
                     /locus_tag="PLB25_07185"
     CDS             complement(6146..6931)
                     /locus_tag="PLB25_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPSS1780 family membrane protein"
                     /protein_id="HOE41398.1"
                     /translation="MKLQLVSARTGATWVKLGIQTFFRQPLALAGLFFMFMALMSVAS
                     MVPVIGLPMAMTLLPAITLGLMAASREAVQGKFPMPLMLLTAFRSGPKKARAMLALGA
                     LYTGGFMMAMGFSYLMDGGDFARMYLGGQMPTSDMINSAEFQSAMWVFLGLHLPLSLV
                     FWHAPALVYWHDMPPIKGMFFSIIACLRNFWAFTVFAALWMVVLALTVLLLTTLSALL
                     DSPNLAGTLLFPALLLVASMFFTSLYFTYRDSFELPVSTPASQ"
     gene            complement(6928..7923)
                     /locus_tag="PLB25_07190"
     CDS             complement(6928..7923)
                     /locus_tag="PLB25_07190"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HOE41399.1"
                     /translation="MAVYTEVSTTEARALLRALKLGELTSLQGCTGGIENTNYFVSTE
                     RDGQAHDYVLTLFERLTIQQLPFYLRLMKHLAERGIPVPDPQADARGELVFEVKGKPA
                     AVVSKLRGKCELRPTVTHCAQVGASLARMHLAGHDFPLSQPNLRALPWWNDTVPVVLP
                     YLTKSQTTLIQQELAYQNHVNASSSFAALPRGPIHADLFRDNVVFDSAQGEAVLSGFF
                     DFYFAGDDTWVFDLAVCLNDWCIDLLTHTHQALLAHSFIASYSAVRPLQRAERELLPA
                     MLRAAALRFWISRLWDFHMPREASILKAHDPGHFEQVLRLRACQAVTFEALTGNI"
     gene            complement(7927..8115)
                     /locus_tag="PLB25_07195"
     CDS             complement(7927..8115)
                     /locus_tag="PLB25_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41400.1"
                     /translation="MPAQAPTEQRVERIHLEDGGSIIDELRIGGETKSINVQPKGGMP
                     AYQVGPVSGERSWKVLGF"
     gene            8306..>8984
                     /locus_tag="PLB25_07200"
     CDS             8306..>8984
                     /locus_tag="PLB25_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HOE41401.1"
                     /translation="MNTAPTPTLLLVDGSSYLYRAFHAMPDLRSVPGDPSSAPTGAIR
                     GIINMLQSLRKEVPAQFAACVFDARGPTFRDVLYPPYKANRSPMPDDLRAQIEPIHEV
                     VRLLGWQVLAVPGVEADDVIGTLAHAAASRGMNVIVSSGDKDLAQLVNERITIIDTMN
                     GRRRDVAGVTAEFGVPPHLMLDYQTLVGDAVDNVPGVPKVGPKTAAKWLNEYGSLDAI
                     LANVDDIK"
BASE COUNT         1952 a         2458 c         2715 g         1859 t
ORIGIN      
        1 taccaggtgg cttattacgc agccgacatg ctggtgttgc tggcgcagtt gcagcccgcc
       61 acactcgact ggctgggcac cagcatgggt ggactgatcg gcatcgctgt agccgggcaa
      121 cttgaactac cggcctttgc caacattcgt cggctggtgc tcaacgatgt ggggccggtg
      181 atcgagtggc aggccttgca gcgcattggc caatatctcg gaagaaccgg caaatttgat
      241 tccgaacagc aggccgctga tgccatctgg acggtttcca gcagttttgg tccacacacg
      301 tcggcccagt ggctggcgct gacccggcca aacctcaagc ccctgggtga cggcagtggt
      361 cggctcacgt tgaattacga tccggcgctg gccctgccat ttgcgacggt gacgcaagag
      421 agtgctgcgc agggcgaggc attgctgtgg caagcctatg accagatcag ggccagtacc
      481 ttgctggttc ggggtgccga ttcggatttg ctttccgaag ccaccgcgca ggccatgacg
      541 cagcgcggcc cgaaagcccg gttaatcgag tttgccggtg tcggccacgc gccgactttt
      601 gtcgcggccg accaagtgca ggccattttg tcattcttgc tggattgaat tgttagccac
      661 agcaaccatt tgtcagaggc gatcaccggt tttggacagc cggattgcct ccagcacaac
      721 tgtaaatcag tgatgagaaa gtcaagcaac gacggcacag tggcagcctc tgcttccaag
      781 tcggtcttgg cggtggtggc tgttacctca tctgacttgt cggcgcaggc caacgcgttg
      841 gtgcgcgcgc gtgcttttgc cgagcccttg ctggtgggcg aaattttcga tactggcgaa
      901 ggtgttttgg ctcatgccga tgcggtcgct gccatactga agggtattgg tggctctgaa
      961 gcgatgcagg ctgctagcta cctggtgtac gttagcgacc atttgaacaa gccgcgcgag
     1021 gtgattggca aggcctttgg cgacaatttt gctgagctgg cgctcgaaac atcaaaattg
     1081 gtgcaggtgc agcggcaggc gcggcgggcg caactggcgc ggcaggcagt cgagcctgac
     1141 aacgcagcgg cccagcaggt ggccgctgtc cagcttgaaa acgtgcgcaa gatgctgctg
     1201 gcgttttcac gcgacctgcg ggtggtgatg ctgcgcttgg catcgcgctt gcagacgctg
     1261 cgattttttg ccgccgccaa gctgccggtg ccgcaggaga tcgcgaccga agcgctgcag
     1321 gtgtttgcac cgctggccaa ccggctcggc atctgggaaa tcaaatggga gatggaggac
     1381 ctggctttcc ggtttctgga gcccgatacc tacaaacaag tggcgcgttt gctagatgaa
     1441 aagcgggtac aacgcgaggc ccatgtcgag cgcatgcggg ttcagcttga acaggatttg
     1501 aacgcccatg gcatcagcgc gcgagtctcg ggtcgcccca aacacatcta cagcattgtg
     1561 aaaaagatgc gtggcaagtc gctcggtttt gagcgggttt ttgacgtgcg cgccatgcgc
     1621 attgtggtgc ccagtgtgcc ggagtgttat ggtgcgttga gctgggtgca cagccattac
     1681 agcccgattg ccgaagaatt tgacgactac attgccaagc ccaaaggcaa tggttaccag
     1741 tctttgcata ccgtggtgcg tgatgccagc ggacaaccta ccgagattca gatccgcacg
     1801 caggccatgc atgaccacgc tgaaaacggt gtggcggcac attgggccta caaggaagct
     1861 ggtgccaaag gctatgccgg ggtgacggcc agcggtgcct acgatggcaa gattgcggta
     1921 ctgcggcagt tgctggcatg ggagcgtgac ctgtcaggcg cgccctcgca agcgcacgcc
     1981 gtgctggacg accgcattta cgtgctgact ccggacgctg ccatcgttga gttgccccgg
     2041 ggcgcgacag ccgtagactt tgcctacgcg gtgcacacca gtctgggtca ccggtgccgt
     2101 ggtgcacggg tggacggggc catggtgccg ttgcacacac cgctcaaaaa tggccaaacg
     2161 gtagaagtga ccactgccaa agagggtgga ccgtcgcgtg actggcttaa ccccgaactg
     2221 ggctacctgg tcagccaccg ggcgcgcacc aaggttcggg cctggttcaa tgcgttggcc
     2281 atgaacgcaa ccattgccaa aggccgcgag gcagttgaaa agatactgca gcgtgagggc
     2341 aaaaccgcgc tcaaacttga cgagctggca gtacaactcg gttttgccaa cgcagaatcg
     2401 ctgtttgaag gggtcggtaa ggacgagttt tcgctgcgca atattgaaca gttgctgcgt
     2461 ccccctgagt ccccgcagtt cgatgccgac gcgccattgt tgcgcaaggc acgctcggga
     2521 gacaatccca ccaagggtgg cttgctggtg ctgggggttg attcgctgat gacgcaaatg
     2581 gccaagtgct gcaagccggc accgcctgac cagatttgtg gctttgtcac ccgaggccgg
     2641 ggggtgagtg tgcaccgcac cgactgcacc aatttgcgcg agatggtggc caaaaacggc
     2701 gagcgcctga ttgaggtcga gtggggcgtg gcgaatgctg tctcccactc ggtttatccg
     2761 gtggatgtgg cggttcaggc gcaagaccgc cagggcctgc tgcgggatat ttcagaggtc
     2821 ttcaccaaag aaaaaatgaa cgtgactggc gtgcaaaccc agtcgatcaa gggcattgcc
     2881 tggatgacat ttaccgtgga agtgtccgat tcaggccgcc tgcacaaggt gctggggctg
     2941 gtgttggagt tgcccggggt gcgctgggcg cgcagaaagt gagcctcgac ttatgacagt
     3001 gacggcttct gctccgatgg cacagcatac gtttttgtgg cacgactatg aaacctttgg
     3061 ggccaacacc cggcgcgacc ggccagcgca gtttggcgcg attcgtaccg atgctgatct
     3121 caatcagatc ggtgacccaa tcgagctgta ctgccagcct gccaatgact ttttgccaga
     3181 tccgcaagct tgcctgatca ccggcatcac gccgcaaatg tgtcttgagc aaggcttgcc
     3241 tgagtaccag tttgcagccc aaatagagca ggcgctggct tggcccggta ccgtgggtgt
     3301 tggttacaac acgatacgct ttgacgatga aatcacccgg ttcatgttct ggcgcaatct
     3361 gatcgaccct tatgcgcggg aatggcagaa cgactgcgga cgctgggacc tgcttgatgt
     3421 ggtgcgcacg gcctatgctt tgcgacctga cggcgtgcag tggccggtcg atcctgatgg
     3481 taagccaagc ttcaagttga ccgacttgac tgccgccaat ggcctggctc atgcgtcagc
     3541 ccacgatgcc ctgagcgacg tgcacgccac gcttgcgttg gcccggttga tcaaacaggc
     3601 acaaccgcgc ttgtttgact tctgcttcgg actgcacaag aaagaccgcg tcatggccga
     3661 gttgggtctg ccgacctcgc tacgcaacgc gctgccgttt tggcatgtgt ctggcatgtt
     3721 cgcgcctggg cgtggttgcc tggcgttgat gtggccgctg gccatgcatc cgggcaacaa
     3781 aaatgagctg ctggcgtggg atttggcaca agacccaggc gagttggtca gcctgaatgc
     3841 ccaacagatc agaacccgcc tgttcaccag gagcgctgat ttgcccgacg gcgtgcgccg
     3901 cctgccaatc aagtcggtac acctcaacaa gtcgccgatg gtcatgggca agctgaaagt
     3961 cctcagtgag gcaatggcac agcgctgggg cattgatctg gatacgcagt ttgcgcatgc
     4021 gcgcaaggcc cgtgacctgc ccgatatgag tgcgatttgg gcgcaggtgt tcaaacgcga
     4081 aggcgatgtg ctgctggacg tggacgagga tttgtatggc ggctttattg gcaacgcaga
     4141 ccgtcggcat ctcaatgagt tacgacaaag taccccggag caactggcga aggcgcgacc
     4201 cgcatttgat gacccccgcc tgagcgaact gctatggcgt tatcgggcgc gaaattttcc
     4261 gcaaagcctc gcgcctgaag aagcaagtcg ttgggaggcg catcgcgcat cgcgtttgtt
     4321 tgaaggcgcg ggcggtgcgc gtaccgtgca ggctctgttt gacgaaatgg acttgctggc
     4381 agagaccgca gacgagcgcg gtgaagctgt gttgggcgcg ctgtacgact atgcggaaat
     4441 gattgcgcca cagcttgcct gattggattt ctgaatcatt cctggcgagg caccctgccg
     4501 aagagattgc atacgcaagg caagacgaga taacaatgat atggatgatt tggcaatgga
     4561 acgagatcag gctgacttct tcggatgccg acatcaaact cctgtgtgat gttctgagtc
     4621 gctaaattgc ctcagcatca tgcaatgtca gctggtactt caaaacaaaa ttggctgtgt
     4681 ttccagggaa acacagccaa ttttggcaat tgccggggca acaaagccag cagcagataa
     4741 gttactggcg cgctggaacc acctgtagca gcttggtgat gtctttgtca ttgggcagtg
     4801 catagtcgtg accgcgaaca atgcgcccat ctccggtgcg tacgaacttc aggcttacat
     4861 aggtcaccgt ggctgcggcc gaataggtgc ccaccagcac atagttggca tgatggctct
     4921 gggcaatttc cttgatctcg cgcgacaaaa gcaactcgcc ggtttcttct ttgacgaata
     4981 cgtcgccgcg cagcttcacc tctttgacgt tgaagcccgc attcaccatg tggcttgcat
     5041 attgctccga cagagtacgg ccaaggggcg cagatcgccg tgtgtcgttg acattgacaa
     5101 ttgtggccac caaaactggc gtatctccca ggggtccaag atcaaagcca gcgaccagtt
     5161 tattgatggc ttctcgagag ctcacaacaa acttgctggc cgcagcatcc tcataagtag
     5221 gttccggtcg cggcggggtg gtatttgcgc agcccaccag aactgccagc gctgttgcgc
     5281 aaagggtgaa tttcatgttc atggacccac caccttgact gtcctgtgaa ctggcaaggt
     5341 cgcaaacagg aattggtctt cttcttcaat gtaatacacg tcagtgttgc gcgtcaaaaa
     5401 acggccaccc gcagcgatcg tggtggtcag aatcaactca gtgtgcgttg gcccacctga
     5461 atcgacgctg gcgacggtgt ccgccagcac ggccaagcca agtccgccga gcaacttggc
     5521 atctgcatgt tcaaggcgca ggccgtaagc ggcgtagagt ccggtagtga gcatggtgaa
     5581 aatgccaggc acaaagtgtg gccttgggct ggcatgacgc accacctggg tctggtaact
     5641 gacttccagc gcgttgtcgg gatattgcac gacaattcga cccgcctgaa ccagttcagt
     5701 gatcaaaaac tcgcgaaacg cacgatcaaa ggcgctgggt tggggtggca gtgtcacata
     5761 caaaggcgct ggcgcactga ccttgtccaa ggtgtcaagg gtctgaacga tgacatcttt
     5821 ggacaaaatg tcccagtgtc cggccgacct taccttgggt tgaacagtga gcgggaagtt
     5881 ctctgacact ggaatcggcg aagtggcgca agctgtcatc agcgccgcgc ccgcacagat
     5941 cgcgagcagc ctcatggttt tgggaatcaa ggtagccccc tcattcgtgg tttaaaagtc
     6001 tgaaactgtt accgatacag caacgcatgc tctcattatc aaccgttctc ttgccagaaa
     6061 acaaggccaa tagctggctt tcaatggttt tttcgctgca aacgctgctc ttgtcgcggt
     6121 gttgccgaca aagataagcc actggctatt gcgatgccgg ggtgctcacc ggtaactcga
     6181 aactgtcgcg ataagtgaaa tacagtgatg taaagaacat tgatgccacc aacagcaacg
     6241 ccgggaacaa caatgtccct gccaggttgg ggctgtccag cagtgctgaa agcgtggtga
     6301 gcaatagtac cgtcagcgcc aagaccacca tccagagagc cgcaaacaca gtgaatgccc
     6361 aaaagttgcg caagcaggca ataatgctga aaaacatgcc cttgattggt ggcatgtcat
     6421 gccaatagac cagcgccggt gcatgccaaa aaaccaatga cagtggcaag tgcaggccga
     6481 gaaacaccca catggctgac tggaactcag ccgaattgat catgtcgcta gtgggcatct
     6541 ggcctcctag gtacatgcgg gcaaagtcgc ctccgtccat cagataggaa aaccccatgg
     6601 ccatcatgaa gccaccggtg tacagagctc caagtgccag catcgcacgc gcctttttag
     6661 gacctgatcg gaaagcggtc agcagcatca acggcatcgg aaacttgccc tggactgctt
     6721 cacgcgatgc cgccatcagc cccaacgtga tcgcgggcaa cagggtcatg gccatgggta
     6781 aaccaatgac aggcaccatg ctcgcgaccg acatcagagc catgaacatg aaaaacaggc
     6841 ccgccagtgc caatggctgc ctgaaaaatg tctgaatacc cagtttgacc caggtcgcac
     6901 cggtacgagc tgacaccaac tgtagtttca tatgttgccc gtgagggcct caaacgtgac
     6961 ggcctggcag gcgcgaagac gcagcacctg ttcaaaatgc cctgggtcgt gagccttcag
     7021 aatgctggct tcgcgcggca tatggaagtc ccataggcgt gagatccaga aacgcaatgc
     7081 ggctgcgcgc aacatggctg gcaagagttc gcgctcggct cgctgcaagg gccgcacggc
     7141 gctgtatgat gctataaaag aatgagctaa tagcgcttga tgggtatggg ttagaaggtc
     7201 aatacaccaa tcattgaggc aaaccgccag atcgaacacc caggtgtcgt cgccagcaaa
     7261 atagaagtca aaaaacccgc tcagtacggc ttcaccctga gccgagtcaa acacgacgtt
     7321 gtcacgaaaa aggtccgcgt ggatcggtcc gcgcggcagg gccgcaaaac tcgatgatgc
     7381 attgacgtgg ttttggtaag ccagttcttg ctgaatcagg gttgtctgac ttttggtcag
     7441 atagggcagc accaccggca cagtgtcgtt ccaccagggc agggcacgca aatttggctg
     7501 actgagtggg aagtcgtgtc cggccagatg catgcgggcc agggaggcac cgacctgagc
     7561 acaatgcgtc acagtgggac ggagttcgca tttgccgcgc agcttgctca ccacggctgc
     7621 gggtttgccc ttgacctcaa acaccaactc gcctctggca tcagcttgcg ggtccggcac
     7681 tggaatgccg cgctctgcca ggtgcttcat cagccgcaaa tagaaaggca attgctggat
     7741 tgtcaaacgc tcaaaaagtg tcagcacgta gtcgtgggcc tgaccatcac gctcggtgct
     7801 gacgaaatag ttggtgtttt cgatgccacc tgtgcagccc tgaagactgg tcagttcacc
     7861 tagtttgagc gcacgcagca gggcgcgcgc ctctgtcgtc gagacctcgg tataaaccgc
     7921 catgactcag aatcccagga ctttccagct acgttcgcca ctgacggggc cgacctggta
     7981 ggctggcatg ccacctttgg gttgtacatt gatggatttg gtttcgccac cgattcgcag
     8041 ctcgtcaatg atcgacccgc catcttccag atggatccgc tcaacccgct gctcggtcgg
     8101 cgcttgggct ggcattgcag tggtgggtgt tggcaagctt gcagcggacg tgtttgcaga
     8161 tgtgccttga ccgctggcaa caccacatag aggtaacgaa aagaccaatg acagtgttaa
     8221 aaaacgcgcg ggtaaaaggt agagctgcat gcccgaattg tagagaaatg ctgaccgcct
     8281 gccattgcac aggcaaaatg ccgtcatgaa caccgcaccc accccaaccc tgctgctggt
     8341 tgatggttcc agctacttgt accgagcctt tcatgccatg ccggatttgc gttcggtgcc
     8401 gggcgatccg agcagcgcgc cgaccggggc gattcgcggc atcatcaaca tgttgcaaag
     8461 cctgcgcaag gaggttccgg cgcagtttgc ggcctgtgtt tttgatgcca ggggtcctac
     8521 ctttcgcgat gttctgtatc cgccctataa agccaaccgc tcgccgatgc ctgacgattt
     8581 gcgggcccag attgagccga ttcatgaggt ggtgcggctg ctgggctggc aagtgctggc
     8641 ggtgccaggt gtcgaggctg acgacgtgat aggcacgctg gcccatgcgg cggccagccg
     8701 gggcatgaat gtgatcgtca gcagtggcga caaagacctg gcacaactgg tcaatgagcg
     8761 catcaccatc atcgacacca tgaacggcag gcgacgcgat gtggccggtg tgacggcaga
     8821 gtttggcgtt ccaccgcacc tgatgctgga ctaccagacg ctggtgggag atgccgtgga
     8881 taacgtaccg ggagtgccaa aagtgggccc caagactgct gccaagtggc tcaatgaata
     8941 cgggtcactt gatgccattt tggctaacgt agatgacatc aagg
//
LOCUS       DAOSLL010000123         8947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7373_length_8947_cov_4.327036, whole genome shotgun sequence.
ACCESSION   DAOSLL010000123 DAOSLL010000000
VERSION     DAOSLL010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8947
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7373_length_8947_cov_4.327036"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="PLB25_07205"
     CDS             complement(<1..406)
                     /locus_tag="PLB25_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOE41402.1"
                     /translation="MEKIGDAASNVAREYLTFRLDREEYGIDILKVQEIRGYESPTRI
                     ANAPSFIKGVVNLRGTIVPIVDMRLKFNCDKAVYDSFTVVIILNLRQRIVGIVVDSVS
                     DVMGLAPENLRAAPDIESVIDSDSVLGLGSLGD"
     gene            complement(443..2659)
                     /locus_tag="PLB25_07210"
     CDS             complement(443..2659)
                     /locus_tag="PLB25_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOE41403.1"
                     /translation="MAEMHHETGSSGADFDLSQFYQIFFEEAGENLDLMEQMLLNLNL
                     ETADDEELNGIFRCAHSVKGGAATFGFSDVAELTHQMESLLDRLRRHELRPTSVMVDV
                     LLESADASRSLLARHQSGDEGTAPPTADLVGRIRKLVSGDTAPAPAPAPASVSAPVKV
                     ETVPVEVQQTEAKSRNQVAAVNVLRTLKVVISRLDHVERADAIEELFRDIAGLGVIKS
                     QACNEANTRVFQVETTSSNEDLLDLFAFHVSREQVVIQETVASSEITDDTGGDASEGN
                     IAGANGPGLDVAHASSTLQAPGFGFFDGAPGAPGAPDASDAGIAAVVAAAPTSEDGLP
                     KSSRSGTKSAGAANSQPEAATIRVAISKVDQLINLVGELVITQAMLAQNSRALDPAVY
                     QQLLTGLTDLDRNTRDLQESVMSIRMIPMSIVFSRFPRMLRDLAGKLGKKVDFLTLGE
                     ATELDKGLVEKITDPLTHLVRNSCDHGIEMPSDRVLAGKPETGTITLSAAHQGGSIVI
                     EVRDDGRGMSRERILAKARERGLDVSDQMQDSEVWQLIFAPGFSTAEVVTDVSGRGVG
                     MDVVKRNIASLNGTVEIDSKEGFGMKVSVRLPLTLAIMDGMSVGVGSEVYILPLSSVV
                     ESFQVKSEAVSTVGQGAQLVKVRDEFMPVIELEKVFQVPRFNTDKTSDIMVVIEAEGS
                     RVVLLVDELLGQQQVVVKNLESNYRKVPNVSGATILGDGKVALILDTGALVRRSRH"
     gene            complement(2706..3068)
                     /locus_tag="PLB25_07215"
     CDS             complement(2706..3068)
                     /locus_tag="PLB25_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE41404.1"
                     /translation="MPVILTVDDSPSMRKMVSFTLVGAGYQVVEAVDGIDAIEKANEQ
                     RFDLVLTDQNMPRLDGLGLTRKLRERPQFKTVPILMLTTESSDLMKQAGRVAGATGWL
                     VKPFDPDRLIEVIKKLIK"
     gene            complement(3109..3549)
                     /locus_tag="PLB25_07220"
     CDS             complement(3109..3549)
                     /locus_tag="PLB25_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41405.1"
                     /translation="MPSDPAFSTPTVKLPVMLVVEVQDSLLGVVHDLNRLDGLLAHAA
                     DRLMQRFTAANASLASATLSDFDDLRQARQTLHSAVTELQFQDIASQLIEHTSKVLQG
                     CAYRLAADAMGFEDGEAVPFVEDVPDRPNPVTQDQMHAGSIELF"
     gene            3622..4755
                     /locus_tag="PLB25_07225"
     CDS             3622..4755
                     /locus_tag="PLB25_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOE41406.1"
                     /translation="MTNKDETPAPLCILHLEDSEHDHLLVRRELGKASEPVKINRVET
                     LSGFSEAVSERQFSVVLADYKLAGFTALDAWDLMQAENIRIPFVLLSGAIGETAAVEA
                     IKAGISDYLPKSDLSKLRHVIQRAIEMHKILLAKERSDHELLQSEQRLAIFAQHLQST
                     IEQERASIAREIHDDIGGSLATIRFDLSWIERHTSDQATLTHVTAATDMLQHAVEASQ
                     RIMMNLRPAILDQGLVPAVHWLASDFTKRTRIETTIRAPKQLENLSRTIQLVAYRTAQ
                     EALTNITKHANCTLVKIDLSDDEGVLTLEISDNGQGAHEIDLSKPQSFGIRGLKERAR
                     TVGGWIDVSSRAGSGMAITLSVPLTEAEKVRQEQQLNDPSNFV"
     gene            4733..5362
                     /locus_tag="PLB25_07230"
     CDS             4733..5362
                     /locus_tag="PLB25_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE41407.1"
                     /translation="MIRVILCDDHAMVRRGIRDTLEEAVDIQVTAEAGSYAEVREAFR
                     LHPCDVLLLDINLPGRGGLEVLASLREVDSPIKVLMVSMFPEDQYAIRCLKAGAQGYL
                     NKAGDPADLITAVRTVAQGRKYVTPGVAQMLVENLSTPKNVSLHASLSERELQTLLKI
                     ASGKRLSDIAQELMLSPKTVSVYRSRVLEKLRLANNSELTVYAIRNGLV"
     gene            complement(5370..6137)
                     /gene="fliR"
                     /locus_tag="PLB25_07235"
     CDS             complement(5370..6137)
                     /gene="fliR"
                     /locus_tag="PLB25_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HOE41408.1"
                     /translation="MITFSEAQLVAWLSPMVWPFLRVLAMFTSAPIFSSRVFPVRARI
                     ALAFFVALAAQPFLGEQAIISITGPAAMGAVVQQLGVGLAIGFTIRMVFAAVELAGEV
                     IGFQMGLSFASFFDPSFNAQTSAVARFFGQMASLLFIVMNGHLLVLLAVLTSFKSFPV
                     DQNFLEALKHMGVLDLGSALFASGLWIALPLIGMLMFANLALGIVSRVAPQMNIYAIG
                     FPITLTVGLFGLAATLPMLDQPFLALMTRAIDIFAVI"
     gene            complement(6151..6420)
                     /gene="fliQ"
                     /locus_tag="PLB25_07240"
     CDS             complement(6151..6420)
                     /gene="fliQ"
                     /locus_tag="PLB25_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HOE41409.1"
                     /translation="MNSQLVLSMGQEALWMLLLISSPVLGIVLVVGLIVSLFQAVTQI
                     NEATLAFVPKLIAAMLVFALAGPWMLGMLVEYIRRTIEAIPASVM"
     gene            complement(6430..7212)
                     /gene="fliP"
                     /locus_tag="PLB25_07245"
     CDS             complement(6430..7212)
                     /gene="fliP"
                     /locus_tag="PLB25_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462884.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HOE41410.1"
                     /translation="MVLFKRPQFVVRQSCLFRLALASLLLTVQSAGLAQGTAQLPLLL
                     GTGSSGVSFSVPVQTLLFFTALSFLPAILLMMTGFTRIVIVLSLLRQALGTQSAPPNQ
                     VVVGLSLFLTLFVMGPTLDKVYNDAYLPYSNNTLPFEQALVRAEAPMRLFMSKQTRQS
                     DLALFVKLGKLDVSTNAETAPMRVLIPAFVTSELKSAFQIGFMIFIPFLVIDIVVASI
                     LMSLGMMMLSPVLVALPFKLMIFVLADGWNLLIGSLAASFVT"
     gene            complement(7215..7478)
                     /locus_tag="PLB25_07250"
     CDS             complement(7215..7478)
                     /locus_tag="PLB25_07250"
                     /inference="COORDINATES: protein motif:HMM:NF016250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HOE41411.1"
                     /translation="MIPVVLKWVQRRVGLAGSGAPTATRIVSAVGVGPHQRVVTVEVG
                     PEGARTWLTLGVTAQSITCLHSVSVMPAQVASANAVGLPADPL"
     gene            complement(7533..7967)
                     /gene="fliN"
                     /locus_tag="PLB25_07255"
     CDS             complement(7533..7967)
                     /gene="fliN"
                     /locus_tag="PLB25_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HOE41412.1"
                     /translation="MATDEKPPEDDGLADWAEALLEQKSAESPSGPEPGGVLTGDSPR
                     GFSSGSGGNDAQINDINRVLDIPVLLSVELGRTKVPIKHILQLGQGSVVELDALAGEP
                     MDVLVNGYLIAQGEVVVVNDKFGIRLTDVVTPSERLRRVSKG"
     gene            complement(7957..>8947)
                     /gene="fliM"
                     /locus_tag="PLB25_07260"
     CDS             complement(7957..>8947)
                     /gene="fliM"
                     /locus_tag="PLB25_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HOE41413.1"
                     /translation="LSQEEVDALLEGVTGESQKLAEEVVESGEVRAYNISSQERIVRG
                     RMPTMEIVNERFARNLRVGLFNFIRRSPEISVGPVTVQRYSAFLRELAVPTNFNIVAI
                     RPLRGSGMVVCEPALVFGVIESLYGGIGKFQTRIEGRDFSATEQRVINRLVDVITQEY
                     KKAWVGIYPLELDYQRSEMQPQFANIATPSEIVVSTSFQLEIGEITGAIHFCFPYATL
                     EPIRDVLYSSTQGDSIEVDRRWVNVLTQEIQAAEVVLVAELAHADATIEQLLAMKPGD
                     FIELDRQPRVQATVDGVPIFECQYGTHNARYALKIERCLRNNDLNWKGERHGN"
BASE COUNT         2390 a         2554 c         2079 g         1924 t
ORIGIN      
        1 gatcacccag cgatccaagc cccaataccg aatcagaatc aataacactc tcaatatcgg
       61 gagcagctct caaattttca ggtgccaatc ccatgacatc gctgactgaa tcgactacta
      121 taccgacaat acgttgcctg agattcaaaa taattacaac agtaaacgaa tcatatactg
      181 ctttgtcgca attaaacttt agacgcatgt ctacaatcgg cacgattgtt ccacgaagat
      241 taaccacacc tttgataaaa gaaggcgcat tggctattcg tgttggtgat tcataacctc
      301 gaatctcttg aacttttaaa atgtcaatac catactcttc cctatccagt cgaaaagtca
      361 aatactcacg tgcaacatta cttgcagcat caccaatttt ttccatgact ttaacctttt
      421 cttaaattgg aatcatcact atctagtgac gggagcgtcg caccaatgca ccggtatcaa
      481 gaataagtgc cactttgccg tcgccaagaa tggtggcccc tgaaacattt ggtactttcc
      541 tgtaattcga ctccaagttt ttaacaacga cctgttgctg ccccaatagt tcatcaacca
      601 acaaaaccac tcgactgccc tccgcttcaa tgacgaccat aatatcgctg gttttatcag
      661 tgttaaatcg tggaacttgg aaaactttct ccaactcaat cactggcata aattcatcac
      721 ggactttaac cagttgcgca ccttgtccca ctgtgctcac tgcttcagac tttacttgaa
      781 atgactcaac aacggacgag agaggcaata tatagacctc acttccgacc ccaaccgaca
      841 tgccatccat aatggccagt gtcaatggca agcgaaccga gaccttcatg ccgaaacctt
      901 ctttggaatc aatttcaacc gtgccattta aggaggcaat atttcgcttt accacatcca
      961 taccgacacc gcggccagaa acatcagtaa cgacctccgc tgttgaaaac cccggggcaa
     1021 atatgagctg ccagacttct gagtcttgca tttgatcaga gacatccaaa cctcgttccc
     1081 gagccttggc caatattctt tcccgagaca tgccccgacc gtcatcacgc acttcgataa
     1141 cgattgagcc tccttggtgt gccgcagaca atgttatcgt cccggtttct ggttttccag
     1201 caagcactct atcgcttggc atttcaatgc cgtgatcaca actgtttcga actagatgag
     1261 tcaatggatc ggtgattttc tcaaccagac ctttgtccaa ttcggtggct tcaccgagcg
     1321 tcaaaaaatc aacttttttc ccaagctttc cagcaagatc cctcaacatc ctcgggaatc
     1381 tgctgaatac aatggacatg ggaatcatgc gaattgacat cactgactct tgcaaatccc
     1441 tggtatttcg atccaaatcg gttaaaccag taagtaactg ctggtatacc gccgggtcaa
     1501 gcgcacggct gttttgcgcc agcatcgctt gtgtaatgac cagttctcca actaagttaa
     1561 tcagttggtc gactttgctg attgcgactc gaatggtcgc agcttcgggc tgcgaatttg
     1621 cagcacctgc agatttagtg ccgctacggg aagattttgg taaaccgtct tcggaagttg
     1681 gtgctgctgc aacgactgcg gcaattcccg catctgacgc atctggtgca ccaggtgcac
     1741 caggtgcacc atcaaaaaaa ccaaaaccgg gagcttgtag ggttgacgat gcgtgagcaa
     1801 cgtccaatcc aggcccattc gcaccagcga tgttcccttc actcgcgtcg ccaccggtat
     1861 catcggttat ttccgaactg gctacagttt cttggatcac gacctgctca cgagaaacat
     1921 ggaacgcaaa cagatccagc aaatcctcat ttgacgaagt agtttccact tggaacacgc
     1981 gtgtatttgc ttcgttgcag gcttgcgact tgatgacccc caacccagca atatcccgaa
     2041 aaagctcttc aatcgcatca gcccgttcaa catgatcgag acggctgatc acaaccttaa
     2101 gggttcgcag aacattcacc gcagcgacct gattccgcga tttcgcctcg gtctgttgaa
     2161 cttcaactgg tactgtttca acctttaccg gtgccgaaac agatgctggc gctggcgctg
     2221 gcgctggcgc agtatcaccc gataccagtt tccgaattct cccgacgagg tctgcggtgg
     2281 gaggcgcagt tccctcgtct ccggattgat gccgggcaag caagctgcgc gaagcatctg
     2341 ctgattccaa cagcacgtct accatgaccg aagtgggtcg caattcatga cgacgcaaac
     2401 ggtcaagcag cgactccatt tgatgagtca actcggcaac atcggaaaaa ccgaatgtgg
     2461 cagcgccgcc tttgacggaa tgtgcacagc ggaagatgcc gttcaactct tcatcgtcgg
     2521 cagtttccaa attcaaattg agcaacattt gctccatcaa atcaaggttt tcccctgctt
     2581 cttcaaaaaa gatttgatag aactggctca ggtcaaaatc tgcccccgaa ctacccgttt
     2641 cgtgatgcat ctcagccata aacactcctg aattcaacgc ctgtggtttt cttgaaaaat
     2701 tcccgttact tgatcaattt cttgatcacc tcgatcagtc gatctgggtc aaaaggctta
     2761 accagccaac ctgttgcgcc agcaactcgc cccgcttgct tcatgaggtc actggattct
     2821 gtcgttaaca tcagaattgg cactgtttta aactgtggcc tctcgcgcaa tttccgggtt
     2881 aacccgagtc catccagacg cggcatattc tgatcagtca acacaagatc aaatctctgt
     2941 tcgttcgctt tttcaattgc atcaatccca tctaccgctt caacgacctg gtagccagca
     3001 ccaaccaaag taaatgacac catcttacgc atggatggcg aatcatcgac agtgagtatg
     3061 acaggcatga aagactccga caaattcaac aacaaaacaa ataaaaattc aaaacaactc
     3121 aatcgatccc gcgtgcattt gatcctgggt aacaggattc ggacggtccg ggacatcttc
     3181 aacaaaggga acagcctccc catcttcaaa acccatcgcg tctgctgcca gccgatatgc
     3241 acacccctga agcactttgg aagtgtgttc aatcagttga gatgcaatat cttggaactg
     3301 caactcggtc acagcagaat gcagggtctg tcgagcttgg cgcaaatcat caaaatcgga
     3361 caaggtcgcc gatgccaggc ttgcattcgc agcggtaaat cgttgcatca aacgatctgc
     3421 tgcatgtgcc agcaaaccat ccaaccgatt caaatcatgt acaacgccta ataacgaatc
     3481 ctgcacttca acaaccaaca taactggcaa cttgacagtc ggtgttgaaa aagcaggatc
     3541 ggagggcatg gaaattcaac tctcttcaga aggcataccg aataaaccgg ctaagtacta
     3601 caaacgatca tagcgccaat catgacaaac aaagacgaaa cacccgctcc tttatgcatt
     3661 ttgcatctgg aagactctga acatgaccat ctgcttgtca ggcgcgagct gggcaaagcc
     3721 agtgagcccg tgaaaatcaa ccgggttgaa accctcagcg ggttttccga ggcggtttcc
     3781 gagcgccaat tcagtgtcgt cctggcagac tacaaattgg cgggatttac cgcgctggac
     3841 gcgtgggatc tgatgcaggc agagaacatc cggatccctt ttgttttgct ttccggcgcg
     3901 attggagaga ccgcagcggt tgaagccatc aaagcgggaa tcagtgatta tttgcccaag
     3961 tctgacctca gcaaactcag gcatgtcatt caacgcgcca ttgaaatgca caaaattctg
     4021 ctggcaaaag agcgatctga ccacgaactc cttcaatcgg aacaacgact ggccattttt
     4081 gcgcagcact tgcagtccac catagagcag gaaagagcgt ctatcgcgcg tgaaattcac
     4141 gacgacattg gcggctcatt ggcgaccatc cgatttgacc tgtcctggat agagcgccac
     4201 acatcagatc aggcaaccct gacacatgtc acagcagcca cggacatgct gcagcacgcc
     4261 gttgaggcca gccaacgcat catgatgaat ttgcgcccag cgattttgga ccaagggttg
     4321 gtccccgccg tccattggct ggccagcgac ttcaccaaac gaacccggat tgaaaccact
     4381 attcgagcgc caaagcaact tgagaacttg tccaggacga tccagttggt tgcctatcga
     4441 acggcgcagg aagcccttac caacatcacg aagcacgcca attgcacgct cgtcaaaatt
     4501 gacctgtctg acgacgaggg agtcctgacg ttggaaatct ctgacaacgg ccaaggggct
     4561 catgagattg atttgtcgaa accccagagt tttggtatcc gtggcttaaa agagcgagcg
     4621 agaaccgttg ggggctggat cgatgtaagc tccagagcag ggtcgggaat ggcgatcact
     4681 ctgtctgttc ccttgacaga agccgaaaaa gtccgacagg agcagcaact caatgatccg
     4741 agtaattttg tgtgatgacc atgcgatggt cagacgtggc atccgggaca ccctggaaga
     4801 ggcagtagac atacaagtga ccgccgaagc aggcagctat gccgaggttc gcgaagcatt
     4861 tcggttgcac ccctgcgatg ttcttttgct tgatatcaat cttcccggac gcggtggact
     4921 tgaggttttg gccagcctgc gtgaggtcga ttcacccatc aaagtactga tggtgtccat
     4981 gtttcctgag gaccagtatg ccattcgctg cctcaaagcc ggggcgcaag gctatctgaa
     5041 caaggcgggt gaccccgctg acttgataac cgctgttcgc acggttgccc agggcagaaa
     5101 atacgtcaca cctggcgtgg cgcaaatgct ggtcgagaat ctcagcacac ccaagaatgt
     5161 aagccttcac gccagcctgt ctgagcgtga actgcaaacc ttgctgaaaa ttgcctcggg
     5221 caaacgactg tctgacattg cccaagagct tatgctcagc cccaaaaccg taagcgttta
     5281 ccggtctcgc gtactggaga agttgaggct tgccaacaac tcggaattga ctgtctacgc
     5341 cattcgcaat ggactggtgt aggccgctct tagatgacgg caaaaatgtc aatcgcgcgc
     5401 gtcatcaatg ccagaaaggg ctgatcgagc atcggcaaag tggcggccag gccaaacagg
     5461 ccaaccgtca aggtgatcgg aaagccaatg gcgtaaatgt tcatttgagg agccacccgc
     5521 gagacgatgc ccaaggccag gttggcaaac atgagcatac cgatcaaggg caatgcaatc
     5581 cataacccac tggcgaaaag tgcggacccc aagtcaagca ctcccatgtg cttcaaagct
     5641 tccaggaaat tctgatcaac aggaaacgac ttgaagctcg tgaggacagc caataacacc
     5701 agtagatggc cgttcatcac aataaacagc aaagaggcca tttggccgaa aaaccgcgca
     5761 acagcgctgg tctgagcatt gaaggagggg tcaaaaaatg aggcaaagct caaccccatc
     5821 tgaaatccga tcacttcccc cgccagctca actgcggcaa ataccatccg gatggtgaag
     5881 cctatggcca agcccacgcc aagctgctgc accaccgcgc ccatggctgc tggaccggtg
     5941 atactgatga tcgcctgctc tcctaaaaac ggctgggcag ccaaagcaac aaaaaaagcc
     6001 agggcgatgc gagcccgaac cgggaacacc cttgaggaga atatcggggc tgaggtaaac
     6061 atggccagca cccgcaaaaa aggccacacc attggcgaca accaggcgac cagttgggct
     6121 tctgaaaatg tgatcacaaa ccggcccgca tcacatcacc gacgcaggaa tggcctcgat
     6181 tgtgcggcga atgtactcca ccagcatccc cagcatccag ggtccagcca gggcaaagac
     6241 aagcatggcc gcaatcaatt taggtacaaa agccagcgtc gcctcgttaa tctgggttac
     6301 cgcctgaaac aggcttacga tcaagccaac caccagaaca atgcccaaaa cgggtgagga
     6361 gatcagcagc aacatccaca gggcctcttg ccccatcgac aataccaatt gcgaattcat
     6421 gtctgaccct caagtgacaa aactggcagc caatgaacca atcagcaggt tccagccatc
     6481 tgcgagcaca aatatcatca gcttgaacgg caacgccacc aaaactggcg acagcatcat
     6541 catgcccagg gacatcaaaa tgctcgcaac cacgatgtcg atcaccaaaa aaggaatgaa
     6601 gatcatgaaa ccaatctgaa acgctgattt cagctcgctc gtcacaaagg cgggtatcaa
     6661 tacccgcatg ggcgcagttt cagcattggt actgacatcg agtttgccca gcttgacaaa
     6721 cagtgcgagg tctgattgac gggtctgttt gctcatgaac agacgcattg gtgcctctgc
     6781 cctgaccagc gcctgctcaa atggcaatgt gttgttgctg tacggcaaat aggcatcgtt
     6841 atagactttg tccagggtcg gccccatgac aaacaaggtc aaaaacaacg acaacccaac
     6901 caccacctga ttcggcgggg cagactgtgt tccgagtgcc tgacgcagca aggacaagac
     6961 aatcacgatg cgggtaaaac ctgtcatcat cagcaaaatg gctggcaaaa atgacagtgc
     7021 agtgaaaaaa agcagggttt gcaccggcac cgaaaagctc acccccgaac tgccagtacc
     7081 caatagcagc ggcaactgag ccgtaccctg cgccaggcca gcggactgca cggtcaaaag
     7141 caggctcgcc agcgccagcc tgaaaagaca gctttgcctg acgacaaact gcggcctttt
     7201 gaatagcacc atcttcacaa tggatccgca ggcaaaccaa cagcattggc gcttgccact
     7261 tgcgcgggca tcactgacac cgaatgcaaa caggtgatcg actgtgcagt tacccccaaa
     7321 gtcagccagg tacgggcacc ctcaggtcca acctcaaccg taaccaccct ttgatgcggt
     7381 ccaacaccga cggccgagac gatgcgcgtc gcggttggtg cccccgagcc cgccaaaccg
     7441 acgcgccgct gcacccactt caatacaaca ggaatcatgg ccaacacaat gacaaaacct
     7501 atgattggca gccaggcctg tgacatgctc gctcagccct tgctgacccg gcgtagacgc
     7561 tccgacggcg tcaccacatc ggttaaccgg atgccgaact tgtcgttcac gaccaccact
     7621 tcaccttgag caatcaggta gccgttcacc agcacatcca tgggctcccc agccaatgca
     7681 tcaagttcaa ccactgaacc ctgaccaagc tgcaaaatat gcttgatggg tactttggtt
     7741 cgccccaatt cgaccgacaa caacaccgga atgtccagca cacgattgat gtcgttgatc
     7801 tgtgcatcat tgccgccgga tccggaagag aaccctctgg gcgagtcacc ggtcaaaacg
     7861 ccaccgggtt cagggccgct cggggattct gcacttttct gctccaatag ggcttccgcc
     7921 cagtcagcca aaccatcgtc ttccggtggt ttttcatcag ttgccatgtc gctcaccttt
     7981 ccagttgagg tcattgttcc tcaggcagcg ctcgatcttt agcgcgtacc tggcgttgtg
     8041 cgtaccatat tgacactcaa aaatgggcac accatcgact gtcgcctgga ccctgggttg
     8101 gcggtcaagc tcgataaagt ctcctggttt catcgccagc agttgctcaa tggttgcatc
     8161 ggcatgcgcc aattctgcta ccagcacaac ctcggctgcc tgaatctcct gagtcagcac
     8221 attgacccag cggcgatcca cctcgatcga atcaccctgg gtcgatgaat acaaaacatc
     8281 ccgaatcggc tccagggtgg cgtacgggaa gcaaaaatga atggcaccgg taatctcgcc
     8341 aatctcaagc tgaaatgatg tggaaacaac aatctcgctc ggggtcgcga tattcgcaaa
     8401 ctgtggctgc atttcggagc gctggtaatc cagctcaagc gggtaaatgc caacccaggc
     8461 cttcttgtac tcctgcgtga tcacatcaac caggcggttg atcacccttt gctcggtggc
     8521 tgaaaaatcg cgtccctcaa tgcgggtctg aaacttgccg ataccgccat aaagcgactc
     8581 gatcacgcca aaaaccagcg ctggctcaca caccaccata ccactgcccc gcaaaggccg
     8641 aatcgccaca atattgaaat tggtcggcac cgcgagttcg cgcaaaaagg cgctgtaacg
     8701 ctgaacggtg acaggcccaa ccgaaatctc gggactcctg cggatgaaat tgaacaagcc
     8761 aacccgtaaa ttgcgggcaa aacgctcgtt gacaatttcc atggtgggca tgcgcccacg
     8821 aacgattctc tcctggcttg agatgttgta agcccgtacc tcgccgctct cgacaacctc
     8881 ttcggccagc ttctggctct cgcccgtgac cccttcgagc agcgcatcta cttcttcttg
     8941 agataga
//
LOCUS       DAOSLL010000124         8891 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7422_length_8891_cov_4.250792, whole genome shotgun sequence.
ACCESSION   DAOSLL010000124 DAOSLL010000000
VERSION     DAOSLL010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8891
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7422_length_8891_cov_4.250792"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1222
                     /locus_tag="PLB25_07265"
     CDS             <1..1222
                     /locus_tag="PLB25_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HOE41414.1"
                     /translation="EQPIAEHHKTRMLELGQWRSMIAQESAKSSKGRKKTAGFHISSL
                     YSPVGWRSWRDVAAAWESAVSKESGSAAAIKTFKNTELGETWLEEGEAPDWQRLIERR
                     EDYRIGSVALGGLLLVAGADVQKDRIEVSIWAFGRGKESWLVEHRVLMGDTARDAVWK
                     RLSELLAERWTHASGVLMPLTRLALDTGFATQETYAFARSCHDPRLMPVKGVRSGAMG
                     GAALIGTPTAVDVTQGGKKLRRGIKLYSVAVGMAKMELYNNLRKAADVGSDGSTPLYP
                     AGFVHLPKVDAEYVQQLCAESLITRHDRNGFAHREWQKMRERNEALDCYVYARAAASS
                     VGLDRFEERHWRELEKQLGLARPPDPEVNVTSSNLSTDAIDASPTEPGRAGISVSGQP
                     KFSRRVIRSPWLKR"
     gene            1310..>1547
                     /locus_tag="PLB25_07270"
     CDS             1310..>1547
                     /locus_tag="PLB25_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41415.1"
                     /translation="MSLQTRLESLVLRLAAEFKTIYGQVGTLANLSTTDKTSLVSSIN
                     ELRSQISTLAGVTIIDDANAAGTATTFSASKITTL"
     assembly_gap    1548..1557
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1558..1864
                     /locus_tag="PLB25_07275"
     CDS             <1558..1864
                     /locus_tag="PLB25_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41416.1"
                     /translation="TTTYSSNKVVTLLDALKADLLGGADAAFDTLKELQDALLNDQTG
                     IAALLAAVDKRVRFDAVQALTAPEQEQARQNIGAVSVLEIGNADTDFVAAFEAALLA"
     gene            1871..2275
                     /locus_tag="PLB25_07280"
     CDS             1871..2275
                     /locus_tag="PLB25_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003278098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41417.1"
                     /translation="MNLAQHITELAQRLALELKTRITADHPGVAKAWVCFGVIGTGSK
                     ASVVVRAGFNVHSVTRTTTGKFRVTFLSPMPDANYCWQAFARNAGNQSAMKHAGARTT
                     AEAKTTQFVEVICTTSNGTLTDTTEMNLTVWR"
     gene            2299..2520
                     /locus_tag="PLB25_07285"
     CDS             2299..2520
                     /locus_tag="PLB25_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019717737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41418.1"
                     /translation="MSFSQTQLEALQAALTQGERRVSFGDKTVEYRSVDELRQAIREV
                     KRGLSEQAASTGMWPGAPRQIRMTTSKGF"
     gene            2520..4052
                     /locus_tag="PLB25_07290"
     CDS             2520..4052
                     /locus_tag="PLB25_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HOE41419.1"
                     /translation="MAWYTKIRSLFGQVGQGPIHEAAGRGRRAQAWMPGNPGAVSALL
                     ATNSELRTKSRDLVRRNAWAQSGIEAFVANAVGTGIKPQSLAGDDTFKAAVQTLWRDW
                     VEEADAAGQTDFYGLQALACRAMLEGGECLIRLRPRRAEDGLSVPLQLQLIEPEHLPL
                     NLNIDLDSGNVVRSGIEFDGMGRRVAYHLYRSHPEDGRLAPMSGQGGLETVRINASEI
                     IHLYKVLRPGQIRGEPWLSRALVKLNELDQYDDAELVRKKTAAMFAGFVTRQSVEDNL
                     LGEGLPDGNGVSLAGLEPGTMQILEPGEDIKFSDPADVGGSYGEFLRAQFRAVAAAIG
                     ITYEQLTGDLSGVNYSSIRAGMLEFRRRCEMVQHDVLVHQMCRPVWAAWMKQAVLSGA
                     LTAPGFARGGNAKRRQYLAAKWIPQGWQWVDPEKEFKAMLLAIRSGLMSRSEAISAFG
                     YDAEDVDREIAADNQRADDLGLIFDSDPRRTSKDGGSAEPNKHAAQVSDNPTSGPVTA
                     DA"
     gene            4062..5330
                     /locus_tag="PLB25_07295"
     CDS             4062..5330
                     /locus_tag="PLB25_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019717739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOE41420.1"
                     /translation="MNLLPHLAARLFGAPLLIHRPKLDVILSVLGSRVGLADLSAPSG
                     FVQPDRSAALANTGAAQSGIAVIPIYGTLVRRTQGLEAQSGLTSYAGIAMALDTALAN
                     PNVAAILLDIDSPGGESSGVFDLADRIRAATAIKPVWAVANDMAFSAAYALASAASRL
                     IVSRTGGVGSIGVIAMHVDQSVKDQQDGIAYTAVFAGDRKNDLNPHAPISGEAHSFLQ
                     GEVNRIYDLFATTVAKHRGMGVNTIKSTQAALYFGADAVASGLADDVGTLDDAIKQIN
                     SMLTPPVPSLSRLLASQTLPETSPEKEIPMTQSVQPTPVLAQTGSTATAPATTSSVEP
                     AFAVSDAIEVAQSCTLAGRTDLIAGFLEAKVAPAQVRSQLLTAMAQQSTEIVSRIDPN
                     AAHRQEMATTNPASPVNPLIAAVKARIGAR"
     gene            5366..5743
                     /locus_tag="PLB25_07300"
     CDS             5366..5743
                     /locus_tag="PLB25_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020749314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HOE41421.1"
                     /translation="MAEIKQTLNLGDLLKYEDEGFYSRDRATLTAGQTLVLGTVLGLV
                     TATGKVKQLDPSATDGSQLACGVLLQDCDAYLVDRDDALMLARHGAVAQHALTWPAAI
                     TVAERDAAVAQLKTVGILVRQSA"
     gene            5812..6828
                     /locus_tag="PLB25_07305"
     CDS             5812..6828
                     /locus_tag="PLB25_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HOE41422.1"
                     /translation="MNNPFLNPAFSMASLTAAINLLPNRYDRLDQLGLFAAKPVRTRT
                     IVLEEKAGVLNLLPSLPVGSPGTVGIRGKRTLRSFVIPHIPHDDVVLPEEVSGLRSFG
                     TENEFASIAAVLAEHLDNMRTKHAATLEYLRMGALKGIVLDADGRVLVNLYDEFKITP
                     KTISFKLNVDTTNVLEKCLDLKRYLGKSLMGERMSSVQCLVSPEFFTKLVTHPSVVDA
                     YRLFNESQILRSDMRSGFPFAGVTFEEYAGEASVPDGSGGWVTKPYIEAGEAHAFPLG
                     TIDTFATYFAPADFNETVNTLGQPIYAKQAPRHFERGTDLHTQSNPLPLCQRPALLVR
                     LTAT"
     gene            6841..7146
                     /locus_tag="PLB25_07310"
     CDS             6841..7146
                     /locus_tag="PLB25_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019717741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41423.1"
                     /translation="MTTLVEKIYLAAANVGFLKICVWQPSDGGAAQTHSVGFSAPDQD
                     VLSGLGVSTEYEMTYPNTCFVGLKSRESVQIEGVAYQVREVTAVGDGSEVRAKLMRV"
     gene            complement(7161..7817)
                     /locus_tag="PLB25_07315"
     CDS             complement(7161..7817)
                     /locus_tag="PLB25_07315"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HOE41424.1"
                     /translation="MATSKPVKGTSNGTRKSLKSQSSPSVFIFKVELVDSEPVIWRRI
                     YLDGRARLNAFHHILQAAMGWTDSHLHEFTIREKRYAPPDEEDQFLEIETLDESKFRL
                     NQLLETGEMFEYLYDFGDSWQHRITVESIRDLDKDDSNAGRVWVESGQRACPPEDAGG
                     IWGYQESLATLEDSPYSEEAKEMREWAGLDFDPERFDRKAANASLDRMLWNGWIKIGV
                     "
     gene            7882..8325
                     /locus_tag="PLB25_07320"
     CDS             7882..8325
                     /locus_tag="PLB25_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019717742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41425.1"
                     /translation="MTQSIREQILQAIVAVLTPVATDQSATVWRTPSVAITREQCPAL
                     VVFPESESLADRANDRVTREVTVRITALARAVPPVIPETQADALLCAAHAALMLDVNL
                     GGLALGVREVESEWEVDDADGVAASTSARYQITYRTLIADISIQA"
     gene            8378..>8891
                     /locus_tag="PLB25_07325"
     CDS             8378..>8891
                     /locus_tag="PLB25_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41426.1"
                     /translation="MSTYASFQGRVYLGKRDVEGNPIEVRSPGNVAELKLSLKTDVLE
                     HYESQTGQRTLDHRMVKQKSATVNLTIEEFTKENLALALYGNFVVGTPGTVTNEPLAG
                     ASPLVGERYFLAHPKVASLVIKDSSATPATLVEGVDYTVDKDFGAIQLLRLNDGGEPA
                     VAYAAPLKASY"
BASE COUNT         1864 a         2665 c         2552 g         1800 t
ORIGIN      
        1 cgagcagcct attgccgagc accacaagac gcgcatgttg gaactgggcc aatggcgcag
       61 catgatcgcg caggagtctg caaagagcag caaaggtcgc aaaaagaccg ccggttttca
      121 catctccagc ctgtacagcc cggtcggctg gcgtagctgg cgcgatgtgg ctgccgcctg
      181 ggagagcgcg gtcagcaagg aatccggttc cgctgctgcg atcaagacct tcaaaaacac
      241 cgaactgggc gagacctggc tcgaagaagg cgaagcaccg gactggcagc gtttgattga
      301 acgccgcgaa gattaccgaa tcggcagcgt tgccttgggt ggcttgctgt tggtggcggg
      361 tgccgacgtg cagaaagacc ggattgaggt gtcgatctgg gcatttggtc gcggcaagga
      421 gtcctggctt gtggagcacc gcgtgctgat gggtgacaca gcacgcgatg ccgtctggaa
      481 aaggttgtca gagttactgg ccgagcgctg gacgcacgcc agtggtgtgt tgatgccctt
      541 gaccaggctg gcgctggaca ctggctttgc cactcaggag acctatgcgt tcgctcgcag
      601 ctgccatgac ccgcgactca tgcccgtcaa aggggtgcgc agcggcgcga tgggtggcgc
      661 ggccttgatt ggtacgccca ctgcggtgga tgtgacccaa ggcggcaaaa agctgcgccg
      721 aggcatcaag ctgtattcgg tggcggtggg catggccaag atggagcttt acaacaacct
      781 gcgcaaagca gctgatgtgg gctctgacgg cagcacgcca ctttacccag ccggatttgt
      841 ccacctgcca aaagtggacg ctgaatacgt gcagcagttg tgcgccgaga gtttgatcac
      901 ccgccacgac cgaaatggct ttgcacaccg tgagtggcaa aagatgcgcg agcgcaacga
      961 ggccctggac tgctatgtgt acgcccgggc tgccgcgtca agtgtcggtc tggaccgctt
     1021 tgaggagcgc cactggcggg agttagaaaa acaactcggg ctggcgcggc cacccgaccc
     1081 cgaggtgaat gtcacctcat caaatttgtc cacagatgcc attgatgcca gcccaaccga
     1141 accaggtcgc gctggcatca gtgtttctgg acagcccaaa ttcagtcggc gcgtgatccg
     1201 cagtccctgg ctcaaacgct aggggccgag gtcattcgtt ggctgtctag ccctggcact
     1261 tcgatgcctt ttttattgcc ttttcatttc cttttttgga gctttcccca tgagtttgca
     1321 aacacgcctt gaatccctgg ttctacgcct tgccgccgag ttcaagacca tctacggtca
     1381 ggtcggtacg ctggccaacc tctcgacaac tgacaaaacc agcctggtca gttccatcaa
     1441 tgagctgcgc agccagattt ccactttggc tggcgtcacc atcattgacg atgccaacgc
     1501 ggctggtacg gccaccacgt tctcggcatc gaagatcacc accttgcnnn nnnnnnncac
     1561 gacgacctat tcgtcgaaca aggtggtcac cttgctcgat gccctcaagg ctgacttgct
     1621 gggtggggcc gatgccgcgt ttgacacgct caaagaattg caggatgccc tgctcaatga
     1681 ccaaaccggc attgctgcct tgcttgcagc cgttgacaag cgagtgcgct ttgatgcggt
     1741 gcaggcactg actgcacctg agcaggaaca agcccgccag aacatcggtg cggtctctgt
     1801 gctggagatc gggaatgcgg ataccgattt tgtggcggca tttgaagctg cgctgctggc
     1861 ctgatcgttc atgaacctgg cccaacacat caccgaattg gcgcagcgcc tggctcttga
     1921 actcaagacc cgcatcaccg cagatcaccc tggggttgct aaagcctggg tgtgcttcgg
     1981 ggtgattgga actggcagca aagcctcggt ggtggttcgg gccgggttca atgttcatag
     2041 cgtcacccgc acaactaccg gcaaattccg ggtgacgttt ttgagcccca tgccagatgc
     2101 caattactgc tggcaggcgt ttgcacgtaa tgcgggaaac caatcggcca tgaaacacgc
     2161 tggcgcacgc accacagcgg aggccaagac cacccagttt gtggaggtca tctgcaccac
     2221 gtcaaacggc acgctgaccg acaccaccga gatgaacctg accgtctggc gctgatgccc
     2281 gaacagaaaa caaccaccat gtccttctca caaacccaac tcgaagcctt gcaagctgca
     2341 ctcacccaag gcgagcgtcg cgtttcattc ggcgacaaga cggtcgaata tcgctccgtt
     2401 gacgaactcc gccaagccat tcgtgaggtc aaacgaggtc tgtctgagca ggctgcatcc
     2461 actggcatgt ggcccggcgc gccgcgccag atccgcatga ccacatccaa aggcttctga
     2521 tggcttggta cacaaaaatc agaagtctat ttggccaggt gggtcaggga ccgatacatg
     2581 aagcagcagg ccgtggcaga cgcgcacaag cctggatgcc cggcaacccg ggcgctgtgt
     2641 cggcgctgct tgccacaaat tccgaacttc gcaccaaaag tcgtgatctg gtgcgccgca
     2701 acgcctgggc gcagtccggc atcgaggctt ttgtggccaa tgcagtcggt accggcatca
     2761 agccacaaag tctggccggt gacgacacgt tcaaggcggc agtgcagacg ctatggcgtg
     2821 actgggttga agaagccgat gcggcaggcc aaaccgactt ctatggattg caagcgctgg
     2881 cctgccgggc gatgctggaa ggtggtgaat gcctgatccg gctgcgaccg cgccgagctg
     2941 aagatgggct gagtgtgccg ctgcaactcc agctgatcga gcccgagcac ctgccactga
     3001 atctcaatat cgatctggat tcaggcaatg tggtcaggtc aggcatcgaa ttcgacggta
     3061 tgggccgacg cgtggcgtac cacttgtacc gatctcaccc cgaagatggc aggctcgccc
     3121 cgatgtcggg ccagggtggc ttggagacgg tgcgcatcaa tgccagtgaa atcatccacc
     3181 tgtacaaggt gttgcgcccg ggacagatac ggggcgagcc gtggctttca cgcgccctgg
     3241 tcaagctcaa tgaactcgac cagtacgacg acgcggaact ggtgcgcaag aagaccgccg
     3301 ccatgttcgc aggctttgtc acccggcaaa gcgtggagga caacctgctg ggcgaaggcc
     3361 tgccggatgg aaacggtgtg tcactggctg gtttggagcc cggcaccatg cagattcttg
     3421 agcccggtga agacatcaag ttctctgacc cggccgatgt gggtggctcc tacggtgaat
     3481 tcctgcgcgc tcagtttcgg gcggttgcag cagccattgg catcacctat gagcaactga
     3541 ctggcgacct ctctggagtg aactattcca gcattcgcgc agggatgctt gagtttcgca
     3601 ggcgttgcga aatggttcag cacgacgtgc tggtgcatca gatgtgtcgc ccggtgtggg
     3661 cggcatggat gaagcaggcg gtgctcagtg gtgccttgac agcgccgggt tttgcccggg
     3721 gtggcaacgc caagcgcagg caatacctcg cagccaagtg gattccgcag ggctggcagt
     3781 gggtggaccc tgagaaggag ttcaaagcca tgctgctggc gattcgctcg gggctgatga
     3841 gtcggtctga agctatttcg gccttcggct atgacgcaga ggacgttgac cgggaaatcg
     3901 ccgccgacaa ccagcgcgcc gatgacctgg gtctgatttt tgactctgac cccagacgta
     3961 cctccaaaga cggcggcagc gcagagccca acaagcatgc agcccaagtg tctgacaacc
     4021 caaccagcgg tccggtcacg gctgacgctt aaaggaattt catgaacctg ttaccgcatt
     4081 tggcggcacg cctgtttggt gcgccgctgc ttatccatcg cccaaaactc gatgtcatcc
     4141 tctccgtgct gggctcgcgc gtcgggttgg ccgatttgtc ggcaccttct ggctttgtcc
     4201 agcctgatcg aagcgcagcc ctggcaaata ccggcgctgc tcagtctggc attgctgtga
     4261 ttcccattta tggcacgctg gtgcggcgca cccagggcct tgaagcgcag tcagggctga
     4321 ccagttacgc cgggattgcc atggcactgg atacagcact ggccaacccg aatgttgctg
     4381 ccatcctgct cgatatcgac tctcccggtg gcgaatcttc tggcgtgttc gatctggctg
     4441 accggattcg cgcggccacg gccatcaaac cggtctgggc ggttgccaac gacatggctt
     4501 tctctgctgc ctatgcgctg gcaagtgcag cgagccgcct gatcgtctcg cgaactggcg
     4561 gtgtgggctc cattggcgtg attgccatgc acgttgatca gtcagtcaaa gaccagcaag
     4621 acggaattgc ctacaccgcc gtttttgccg gtgaccgcaa aaacgatctc aacccgcacg
     4681 caccgatctc gggtgaagca cacagctttt tgcagggtga agtcaaccgc atttatgacc
     4741 tgttcgccac gacggtggcc aaacacaggg gcatgggcgt gaacaccatc aaaagcaccc
     4801 aagcagccct gtacttcggc gcagacgcgg ttgcttctgg tctcgctgat gacgtgggga
     4861 cgcttgatga cgcgatcaag cagatcaatt ccatgctgac cccaccagtt ccctcgctct
     4921 cccgactgct tgccagtcaa accctccctg aaacctcacc tgaaaaggaa attcccatga
     4981 cgcaatccgt ccaacccacc cctgtgctcg cccaaactgg tagcactgcc accgctccgg
     5041 ccaccacatc atccgtcgaa ccagcattcg cagtctctga tgcgattgaa gtcgcgcaaa
     5101 gctgcaccct ggctggtcga actgacttga tcgctggctt tttggaggcc aaagtggcac
     5161 ccgctcaggt gcgcagccag ttgctcacgg caatggctca gcaatccacg gaaatcgtca
     5221 gccgcatcga cccgaatgct gcacaccgcc aggaaatggc gacaaccaac cctgcttcgc
     5281 ctgtcaaccc gctgattgcc gccgtcaaag cccgaatcgg tgctcgctaa atcccacctg
     5341 accgcctcac taataggaga actccatggc tgaaatcaaa caaaccctca atctgggcga
     5401 cttgctcaag tacgaagacg aaggctttta ctcgcgcgac cgcgccaccc tgactgctgg
     5461 ccaaaccctg gtacttggca ccgtacttgg gctggtgacc gccaccggca aggtcaaaca
     5521 acttgatcca agtgccaccg atggcagcca actcgcctgt ggtgtgttgt tgcaggactg
     5581 cgatgcctac ttggtggacc gggacgacgc actgatgctg gcgcgccatg gcgcggtggc
     5641 ccaacacgca ctcacctggc ctgcggccat caccgtggca gagcgcgatg cagcagtggc
     5701 ccagctcaag accgtgggca ttctggtgcg ccaaagcgcc tgatatccgc tttgatctgc
     5761 aagccaattc gccccactca tcccactttt tgaaggaaag ccatcatggc catcaacaac
     5821 ccgtttctca atcctgcttt ttcaatggca tcgctgacag ccgcgatcaa cctgctgccc
     5881 aatcgatacg accgactcga ccagttgggt ctttttgccg ccaagccggt gcgcacccgc
     5941 accatcgtgc tcgaagaaaa agccggtgtg ctcaatttgc tgcccagcct gcctgtgggc
     6001 tcgcctggca cggtcggtat tcggggcaaa cgcacgctgc gcagctttgt gatcccgcac
     6061 atcccgcacg atgacgtggt gctgcctgaa gaagtctctg gcctgcgctc cttcggtacc
     6121 gagaacgaat ttgcctccat tgcagcggtg ctggccgagc acttggacaa catgcgcacc
     6181 aagcatgcgg ccaccctgga gtacctgcgc atgggcgctt tgaaaggcat cgtgctcgac
     6241 gcggatggcc gggttctggt gaatctgtac gacgaattca agattacgcc caaaaccatc
     6301 agcttcaaac tcaacgttga caccaccaac gtgctggaaa agtgtctcga tctcaagcgc
     6361 tacctcggta aaagcctgat gggtgagcgc atgagcagcg tgcagtgctt ggtctcgccc
     6421 gagttcttca ccaagttggt gacccacccc agcgtggtcg atgcgtacag gctgttcaac
     6481 gagagccaga ttctgcgctc cgacatgcgc tcgggcttcc cgtttgctgg cgtcaccttt
     6541 gaggaatacg ccggtgaagc cagcgtaccc gatggctccg gtggctgggt gaccaaaccg
     6601 tacatcgaag cgggcgaagc gcatgcgttc ccgctgggca cgattgatac ttttgccacc
     6661 tactttgcac cggccgactt caacgagacg gtcaacacgc tgggacagcc gatatatgcc
     6721 aagcaggcac cgcgccattt tgagcgtggc actgacctgc atacccaaag caacccgctg
     6781 cccctgtgcc agcgtccagc cctgttggtg cgcctgaccg cgacgtgatc gggtcaaacc
     6841 atgacgacac tggtcgaaaa aatctatctg gccgctgcca acgtggggtt tctcaagatc
     6901 tgcgtctggc aacccagcga tggcggtgca gcgcagacgc attcggtcgg attttctgca
     6961 cctgatcaag acgtgctctc agggctcggt gtcagcactg aatatgagat gacctacccg
     7021 aacacttgct ttgtggggct caaatcccgc gaatcagtgc agatcgaggg tgtcgcgtat
     7081 caggtgcgag aagttacggc cgtgggcgac gggtcagagg tgcgcgccaa actgatgcgg
     7141 gtgtgatcaa tacgcttctc tcagacacca atcttgatcc acccattcca aagcatgcga
     7201 tccagggagg cgttggcagc cttgcggtca aagcgttcgg ggtcgaagtc cagtccagcc
     7261 cattcgcgca tttctttggc ttcttcgctg taagggctgt cctcaagggt ggccagagat
     7321 tcctgatagc cccaaatgcc accagcgtct tcgggtgggc aggctcgctg acccgattcc
     7381 acccataccc ggccagcgtt gctgtcgtcc ttgtccagat cgcgaattga ctccaccgtg
     7441 atccggtgct gccaactgtc gccaaagtcg tacaggtatt caaacatttc acctgtttcc
     7501 agcagttgat tgagacggaa tttggactca tccaaggttt cgatttccag aaactggtct
     7561 tcctcgtcgg gaggtgcgta gcgcttctca cgaatcgtga attcatgcaa atgtgagtca
     7621 gtccaaccca tggccgcttg caggatgtgg tgaaacgcat ttaggcgtgc gcggccatcg
     7681 aggtaaatgc gtcgccagat cacgggctct gagtccacca actcgacctt gaagatgaaa
     7741 acactcggag atgactggga tttgaggctt tttctggtgc cgttgctggt gcctttgacg
     7801 ggctttgatg tcgccatgaa gagtgcctct ttgagctcaa aacaagcatt ttatgaatga
     7861 cccttaacca acaccgaaac catgacccag tccattcgcg aacaaatcct gcaagcaatc
     7921 gtcgcggtac tgaccccagt ggccaccgat cagtcagcca ccgtctggcg aacacccagt
     7981 gtggccatca cgcgggagca gtgcccagcg ctggtggtgt ttcctgagag tgagtcactg
     8041 gcagatcgcg ccaacgaccg ggtcacccgc gaggtgaccg tgcgcatcac cgcactggcg
     8101 cgcgccgtac caccggtcat cccagaaacc caagctgatg ccttgctctg tgcggcgcat
     8161 gccgccctga tgcttgacgt caacctgggc ggtctggctc tcggtgtcag ggaagtcgag
     8221 tccgaatggg aagtggatga cgcggacggg gttgctgcca gcacatctgc tcgctaccag
     8281 atcacctacc gcaccctgat tgcggacatt tccattcaag cctgaatcac attttcattt
     8341 ttctgtttag tcattccaac tttaaggatc ccaaaccatg agtacctatg catcattcca
     8401 gggccgtgtt taccttggca aacgtgacgt tgaaggcaac cccatcgagg ttcgctcacc
     8461 cggcaacgtg gccgagttga aactctcgct caaaaccgac gtgctggagc attacgagag
     8521 ccaaaccggt cagcgcacgc tcgatcaccg catggtcaaa caaaagtccg ccacagtaaa
     8581 tctgaccatc gaagagttca ccaaagaaaa cctggcgctg gccctgtacg gcaacttcgt
     8641 ggtcggtaca cccggcactg tgaccaatga gccattggca ggcgcgtcac cgctggtcgg
     8701 tgagcgctat ttcctggcac accccaaggt ggccagcctg gtgatcaagg acagcagcgc
     8761 cacaccggcc actttggtcg aaggggtgga ctacaccgtc gacaaggact tcggcgcaat
     8821 ccagttgctg cgtctgaacg atggcggcga gcctgccgtg gcctacgctg cgcccttgaa
     8881 agccagctac g
//
LOCUS       DAOSLL010000125         8884 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7429_length_8884_cov_3.900102, whole genome shotgun sequence.
ACCESSION   DAOSLL010000125 DAOSLL010000000
VERSION     DAOSLL010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8884
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7429_length_8884_cov_3.900102"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="PLB25_07330"
     CDS             complement(<1..532)
                     /locus_tag="PLB25_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE41427.1"
                     /translation="MQKLVLITGASSGIGQALALQYAREGYRLALLARRVDVIKSWLG
                     PYAISADSYCIYSADVSDASSMASAAQDCLMRQGVPDVVIANAGISIGVDSSRREDID
                     VMARILATNVVGMAATFGPFIDPMVARGSGTLVGIGSVAGIRGLPGHGGYCASKAAVI
                     SYCESLRGELRSTGVKV"
     gene            complement(538..1155)
                     /gene="lptC"
                     /locus_tag="PLB25_07335"
     CDS             complement(538..1155)
                     /gene="lptC"
                     /locus_tag="PLB25_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HOE41428.1"
                     /translation="MINLLRRVWDRVTLYLPVFLMGVLAMATYWLVRSTPIVARPRPV
                     AAAQHKPDYFMRNFAVKTFDAAGKLKSEVKGTEARHYPDTNMLEIESVQIRSFDVQGR
                     LTTASARLAITNEDASEVQLIGTAQVMRAPVAGPAGVAEPALTFAGEYLYAFIHAERV
                     ISNKPVELTRGQDRFTADNMDFDNLQRVMLLKGRVKGILLPAARP"
     gene            complement(1152..1697)
                     /locus_tag="PLB25_07340"
     CDS             complement(1152..1697)
                     /locus_tag="PLB25_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006299035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HOE41429.1"
                     /translation="MTPALNFLPELLLKAQGIRVVFFDVDGVLTDGGLYFSEHGETLK
                     RFSTLDGHGLKLLQRAGITPAVITGRDSKPLRLRLAALGVVHVHYGTEDKRPAAETTL
                     KALGLDWSQAAAMGDDWPDLPVMTRCALACAPANAQAELLAMAHFVTQRQGGAGAVRE
                     LCDLLLVASGRYAQLLQESLK"
     gene            complement(1697..2698)
                     /locus_tag="PLB25_07345"
     CDS             complement(1697..2698)
                     /locus_tag="PLB25_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HOE41430.1"
                     /translation="MMFEPPTPTAVDAARAIALAHETLDIEAAAVLGIKRHLGDAFVQ
                     AMRLMLMVRGRVVVMGMGKSGHIGRKIVATLASTGTPAMFVHPAEASHGDLGMIQPID
                     LVLAISNSGESKELTDILPMIMRLGAPLVAMTADAQSTLARHADVFLNCGVDREACPL
                     NLAPTASTTAQLALGDALAVALLDARGFKAEDFARSHPGGALGRKLLTHVSDVMRTGD
                     AVPRVTLDASFSDLMREMSAKGLGATAVLDDAGGLQGIFTDGDLRRLVEKGIDLRSTT
                     AGQVMHPNPSTVSLDALAVEAVELMELRRITSVLVLDATGQLCGALNSNDLLRAKVI"
     gene            2764..4749
                     /locus_tag="PLB25_07350"
     CDS             2764..4749
                     /locus_tag="PLB25_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HOE41431.1"
                     /translation="MDPLDLTLLYLLAAVLGVVACRYLKLPPMLGYLAVGVVIGPNAL
                     ALAKNADGVRHLGEFGVVFLMFVIGLEFSLTKLRAMRNHVFGLGLLQVALTIIITTLG
                     SLGLAWLAPKLWGMSWQSALALSSALAMSSTAIVVKLMTERIEMESEHGKRVMGVLLF
                     QDLAVVPLLVLIPALGASANALLHTMTLALFKATALITLLLVGGPRVMRWWLTLVARR
                     KSEELFVLNLLLVTLGLAWLTELAGLSLALGAFIAGMLISETQYKHQVETDIRPFHDV
                     LLGLFFISIGMMLDWRLVLERWPLVLVLVVLPVLFKAVLMTGIARGFGASLGVSLRTG
                     LYLAQAGEFGFVLLALAQTHALIAPELLNPILAAMVLSMLATPFIIMHSNALVLRLVA
                     NEWLMEAVQMTSIARKSIKSNKHVIICGFGRCGQNLARMLDKENIPYMALDLDPDRVR
                     QAAAAGDSVVFGDAARLQALMAAGLARASAVVVTYLETASAMRVLAHIHEHAPKVPAI
                     VRTQDDHDLELLQRAGATEVVPEAIEGSLMLATHALALVGVPMRRVIRIVQEQRDARY
                     SLLRGYFRGADDGTSDETDQERLSSLSLPRQARSIGRRVDALMLDALGVQLVSIRRKD
                     GQVMARTDELVLMEGDALVLSGKADPLKQAEHYLLKG"
     gene            4785..5339
                     /locus_tag="PLB25_07355"
     CDS             4785..5339
                     /locus_tag="PLB25_07355"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013521139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HOE41432.1"
                     /translation="MQNISVNQYLREHIRTVRDWPAPGVQFRDITPLLQDAKVFRVLI
                     DAFVHRYMDPQHRPDVVAGLDARGFIVGAVVAYELGVGFVPIRKKGKLPFTTVEQTYE
                     LEYGSATVELHVDAVRPGNRVLLIDDLIATGGTMLAGKMLLEKLGAIVTEGAAIVDLP
                     DLGGSARLRSSGLALFSLVDFDGQ"
     gene            complement(5372..6112)
                     /locus_tag="PLB25_07360"
     CDS             complement(5372..6112)
                     /locus_tag="PLB25_07360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41433.1"
                     /translation="MSVFSWLFSNAKSSSGPGPQSDGPTAAQPGLAPAAHQSLAELKQ
                     QRQLRRDNLYSVVREVMLRSEVLASNYKFKVLSLDTQGRQFLIMVDLADEKGLPPSQL
                     TNIEQLLGSTALQRHNLLVRSVYWRQTEIQPAPVVAAPRAASRPVSASAPTAAPENAP
                     APVIDVAAPTPRGRGGAKKAGFDPIGQDEVLAFKRAIANAPAPARSDQPVVSGLRNSA
                     AMTGFEDTQLLEPDDTASPLSSTQFGDL"
     gene            complement(6209..7615)
                     /gene="mnmE"
                     /locus_tag="PLB25_07365"
     CDS             complement(6209..7615)
                     /gene="mnmE"
                     /locus_tag="PLB25_07365"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HOE41434.1"
                     /translation="MSLPRHQDPIAAVATAPGRGAVGIVRVSGRGLAGLIWALCRRTL
                     KAREATYTAFLDKDGVAIDHGLAIFFAAPHSFTGEDVLELQGHGGPVVLQLLLARCLE
                     AAAQAAADGAPHLASLRLAMPGEFSERAYLNDKIDLAQAEAIADLIDASTEAAARSAS
                     RSLSGAFSQEIHRLRDALVHLRVQIEATLDFPEEEIDFLHQTNAAGQLLRLQQTLNTV
                     MQKARQGALLREGIKVVIAGQPNAGKSSLLNALAGAELAIVTPVAGTTRDRVQQTIQI
                     EGVPLHVIDTAGLRDSDDEVEKIGIARAWDAIAHADAVLFLHDLTRQNAPQYIAADAD
                     IIRAISKKLPQHVVVIEVWNKADAAAPSALASGLLLSAKTGLGLDKLRHELLAVAGWQ
                     SATEGMYMARERHVSALRRVHAHLASAQSYLALQAQALDLLAEELRLAQNALNDITGE
                     FTSDDLLGIIFSSFCIGK"
     gene            8142..>8884
                     /gene="glgA"
                     /locus_tag="PLB25_07370"
     CDS             8142..>8884
                     /gene="glgA"
                     /locus_tag="PLB25_07370"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HOE41435.1"
                     /translation="MKVLLITREYPPYVYGGAGVHVEYLAAELAKRMHVEVRCFGDQD
                     TVKEGVRVKGFPFGKGSFSHVDKRLSGALDTFETNLETLSEQIDADLVHVHTWYAHLG
                     GILAKTLYGIPLVHTVHSLEPLRPWKREQLGCGYDMSSWIERTSLGLADAVIAVSEGT
                     KADILEHFDIDPAKITVIYNGIKVDQYHPSEDTDALVKYGVDPERRTVLFVGRITRQK
                     GILHLVNAVKYINPDVQIVDISPNDRRDY"
BASE COUNT         1749 a         2612 c         2673 g         1850 t
ORIGIN      
        1 gcaccttgac cccggttgag cgcaattcac cacgcaggct ctcgcagtag ctgatcaccg
       61 ccgccttgct ggcgcagtag ccgccgtggc cgggcaagcc ccggatgccc gccacgctgc
      121 cgatgcctac caaggtgccc gagccgcgcg ccaccatggg gtcgataaat gggccaaaag
      181 tcgctgccat gccaacgaca ttggtcgcca gaatgcgagc catcacgtcg atgtcttcgc
      241 gacgcgagga gtccaccccg atgctgatgc cggcgttggc gatcaccaca tcgggcaccc
      301 cctggcgcat cagacagtcc tgcgcggcgc tggccatgct gctggcatca gatacatcag
      361 cactataaat acaatagctg tcagcgctta ttgcataagg gccaagccat gatttgatca
      421 catccacacg gcgcgccagc agcgccagac ggtagccttc tcgggcgtac tgcaaggcca
      481 gagcctggcc gatgccgctg gatgcaccgg tgatcagaac gagtttttgc atggtgttca
      541 ggggcgcgca gcgggcaaca aaatgccttt cacccgtcct ttgagcagca tgacccgctg
      601 caggttgtca aaatccatgt tgtcagcggt gaaacggtct tggccacggg tcagttcgac
      661 cggtttgttt gatatgacgc gctcggcgtg aatgaatgca tacaggtact caccggcaaa
      721 tgtcagggcc ggttcggcca cccctgcggg tcctgccacc ggtgcgcgca tcacttgcgc
      781 tgtgccaatc aactgaactt cagaagcatc ttcgttggtg atggccagtc gcgcactggc
      841 agtggtcagg cggccctgga catcaaacga gcgaatctgc accgactcga tttccagcat
      901 gttggtatcc ggataatgtc gcgcctcggt gcctttgacc tcgcttttga gtttgcccgc
      961 cgcgtcaaac gtcttgaccg caaagttgcg catgaaatag tcgggcttgt gctgtgcggc
     1021 agcaactggt cggggccgcg cgacgatcgg cgtgctgcgc accagccaat aagtggccat
     1081 ggccagcaca cccatcaaga ataccggcaa atacagggtg actcggtccc agacccggcg
     1141 cagcaggttg atcacttcag cgattcctgc aaaagctgcg catagcgccc gctggcgacc
     1201 agcaacagat cacacaattc gcgcaccgca cccgcacccc cttgtcgctg tgtcacaaag
     1261 tgtgccatgg ccagcagttc cgcctgtgca ttggctggtg cgcaggccaa agcgcagcgc
     1321 gtcatgaccg gcaagtccgg ccagtcatcg cccatggcgg cggcttgtga ccaatccagc
     1381 ccgagtgcct tgagcgtggt ctcggcggcc gggcgcttgt cttcggtgcc atagtgaaca
     1441 tgtaccacac ccagcgcagc cagccgcagc cgcaacggtt tcgagtcgcg cccggtgatc
     1501 acagcgggcg tgatcccagc gcgctgcagc agtttgaggc cgtgaccatc gagcgtgcta
     1561 aagcgtttga gcgtctcgcc gtgctcggaa aagtaaaggc cgccgtcggt cagcaccccg
     1621 tcgacatcaa aaaaaacaac ccggataccc tgcgccttga gcagcagttc aggcaggaag
     1681 ttgagtgccg gggtcatcag atcaccttgg cgcgcagcag gtcattgctg ttgagcgcac
     1741 cgcacagttg cccggtggca tcaagcacca gcacgctggt gatgcgcctg agttccatca
     1801 gttcgaccgc ttccaccgcc agcgcatcaa gactgacggt gcttggattg gggtgcatca
     1861 cctgcccggc tgtggtactg cgaaggtcaa tgcctttttc gaccaggcgg cgcaagtcgc
     1921 cgtcggtgaa tatgccttgc aagccccccg catcgtccaa aacggctgtg gcacccagac
     1981 ccttggcgct catttcgcgc atcaggtcgc tgaaactggc atccagcgtg accctgggta
     2041 cggcatcccc ggtgcgcatg acatcgctga catgggtcag caatttgcgc ccgagcgcgc
     2101 cgcccgggtg cgagcgggcg aaatcttcgg ctttgaagcc acgggcatca agcaacgcaa
     2161 ctgccagcgc atcgcccaga gccagttggg cagtggtact ggcggtcggg gccaaattga
     2221 gtggacaggc ttctctatcg acaccgcagt tcaggaagac atcggcgtgg cgcgccagcg
     2281 tggattgggc atctgccgtc atggctacca gcggcgcgcc caggcgcatg atcatcggca
     2341 aaatgtcggt gagctctttc gactcgccac tgttggaaat ggccagcacc aggtctatcg
     2401 gctgaatcat gcccagatcg ccatggctgg cttcggccgg gtgaacaaac atggccgggg
     2461 tgccggtgga ggccagggtg gcaacgatct tgcggccaat atggccgctc ttgcccatgc
     2521 ccatcaccac cacacgacca cggaccatta gcatcaaacg catcgcctgg acaaaggcat
     2581 cacccaggtg gcgcttgatt cccagcacgg cggcggcctc gatgtcgagc gtttcatgcg
     2641 ccaaggcaat ggctcgggcg gcatcaaccg ctgttggggt gggtggctca aacatcatgg
     2701 tggcatttta tcggtcatgc ggcttggcac ggcattgtct ggtagcttgg tagtatcaag
     2761 gttatggacc cgcttgatct gacccttttg tatttgctgg ctgccgtact cggcgtggtg
     2821 gcctgtcgct acctgaagct tcccccgatg ctgggttacc tggcagtggg ggtggtgatt
     2881 ggcccgaacg cgctggcgct ggccaagaat gcggatggcg tgcggcatct gggtgaattc
     2941 ggggtggttt ttctgatgtt tgtgattggc ctggagttca gcctgaccaa gttgcgtgcc
     3001 atgcgcaacc atgtttttgg gctgggtttg ctgcaggttg cactcaccat catcatcacc
     3061 acgctgggta gcctgggcct ggcctggctg gcacccaaac tgtggggcat gagctggcag
     3121 tcggcgttgg cgctgtcaag tgccctggcc atgagcagca ccgccatcgt cgtcaagctg
     3181 atgaccgagc gcatcgaaat ggagagcgaa cacggcaagc gtgtcatggg cgtgctgctg
     3241 tttcaagact tggctgtggt gccgctgctg gtgctgattc cggcgctggg cgcgtctgcc
     3301 aacgcattgc tgcacaccat gacactcgca ctgttcaagg ccacggcgct gatcaccctg
     3361 ctgctggttg gtgggccgcg cgtgatgcgc tggtggctga ccctggttgc ccggcgcaaa
     3421 agtgaagagc tgttcgtgct gaacctgctg ctggttaccc tcggcctggc ttggctgacc
     3481 gaactggcgg gcctgagtct ggcgctcggg gcattcattg ccggcatgct gatttctgaa
     3541 acccagtaca agcatcaggt ggaaaccgac atccgcccgt ttcacgatgt cctgctgggc
     3601 ctgtttttca tcagcatcgg catgatgctg gactggcggc tggtacttga gcgctggccc
     3661 ttggtgctgg tgctggttgt cctgccggtg ctgttcaaag cggtactgat gacagggatc
     3721 gcacgcggct ttggtgccag cctgggggtt tccctgcgta ccgggctgta cctggcacaa
     3781 gccggtgaat tcggttttgt gttgctggcg ctggcgcaga cccacgcgct gattgcgccc
     3841 gaactgctca acccgattct ggccgcaatg gtgctgtcga tgctcgccac acctttcatc
     3901 atcatgcaca gcaacgccct ggtactgcgc ctggttgcca atgaatggct gatggaagcg
     3961 gtgcagatga ccagcattgc ccgcaaatca atcaagagca acaaacatgt gatcatttgc
     4021 ggtttcggcc gttgcggcca gaacctggcg cgcatgctcg acaaggagaa cattccttac
     4081 atggcactcg acttggaccc cgaccgggtg cgtcaggctg ctgctgccgg tgactcggtg
     4141 gtgtttggcg atgcggcgcg cctgcaggca ctgatggcgg ccgggctggc ccgtgccagt
     4201 gcggtggtgg tgacgtatct ggaaaccgcc agcgccatgc gtgtgttggc ccatattcac
     4261 gagcatgcac ccaaggtgcc agcgattgtg cggacccagg acgaccacga tctggaactg
     4321 ctgcagcgtg ccggtgccac cgaggtggtg ccagaagcga tagagggttc attgatgttg
     4381 gccacccacg cgctggcact ggtcggtgtg ccaatgcgcc gcgtgatccg tatcgtgcag
     4441 gagcagcgtg atgcccgcta cagcctgttg cgcggctact ttcgcggtgc tgatgatggc
     4501 accagcgacg aaaccgatca ggagcgcctg agctcgctga gtctgccgcg tcaggccagg
     4561 tcaattggtc gccgggttga tgcgctgatg ctggatgcgc ttggcgtgca gttggtcagt
     4621 attcggcgca aggacgggca ggtcatggca cgcaccgacg aactggtatt gatggagggc
     4681 gacgcgctgg tgctatcggg caaggcagac cctctcaagc aagctgaaca ttacttgctc
     4741 aagggctgac gcacaatcag agccatcgtt tctttggatt tctcatgcaa aacatcagtg
     4801 tcaaccaata cctgcgcgaa catattcgta ccgtgcgcga ctggcccgca cccggggtgc
     4861 agtttcgcga catcacgccc ctgttgcagg atgccaaggt gtttcgggtg ttgattgacg
     4921 cttttgtgca ccgctacatg gacccgcaac atcgccccga tgtggtggcc ggactcgatg
     4981 cacgcggctt tatcgtcggc gctgtcgttg cctatgaatt gggtgtcggt tttgtgccga
     5041 tccgcaagaa aggcaagctg cctttcacca cggtggagca aacttacgaa ctcgagtacg
     5101 gcagcgccac agttgagctg catgtcgatg ccgtcaggcc cggcaatcgc gtgctgctga
     5161 tagatgacct gattgccacc ggcggcacca tgctggcggg aaaaatgctg ctggaaaaac
     5221 ttggtgccat agtcaccgaa ggtgcagcca ttgtcgattt gcccgacctc ggtggctcag
     5281 ccagactgcg cagcagtggc ctggcgcttt tttccctggt cgatttcgac gggcaatgag
     5341 cccgccggca gttgtcacag gtttggcggc tctacaaatc cccgaactgg gtgctgctca
     5401 agggtgaagc cgtgtcatct ggctcaagca actgggtatc ctcaaaacca gtcatggcgg
     5461 ctgaattgcg cagtccagac accacgggct ggtcggacct ggctggcgca ggggcattgg
     5521 cgatcgctct tttgaatgcc agcacctcgt cctgaccaat cggatcaaag cccgcttttt
     5581 tggccccgcc ccggcccctg ggcgttggtg ccgcaacatc aatcactggt gccggtgcgt
     5641 tttcaggtgc tgccgtcggc gcacttgcag ataccggccg agatgccgcc cgaggtgccg
     5701 caacgaccgg tgctggctga atttcggtct gacgccagta caccgatctc accagcaagt
     5761 tgtggcgctg cagggcggta ctgccaagca attgttcaat gttggtcaac tggctcgggg
     5821 gcaggccctt ttcgtcggcc aggtccacca tgatgagaaa ctggcgtccc tgtgtatcaa
     5881 gcgacaacac cttgaattta tagttggaag ccagcacctc gctgcgcaac atgacttcac
     5941 gcaccacact gtagagattg tcccgcctga gttggcgctg ttgtttcagc tcagccagcg
     6001 actggtgggc cgctggtgcg aggccgggct gcgcggcagt cgggccatca gattgagggc
     6061 ctgggcctga tgacgacttg gcattggaaa ataaccagct aaatacggac acgatcaacc
     6121 ccttgaaaag tatgacaaat ttcaggcatg cagaaaacct gaatgccagt caaaccgcgc
     6181 ccaggtattg cagcttgggc cgggatgctc acttaccgat acaaaaactc gaaaaaatga
     6241 tacccagcaa atcgtccgag gtgaattcac cagtgatgtc attgagtgca ttttgcgcca
     6301 aacgcaactc ttcggccagt aaatccagcg cctgcgcctg cagcgccagg tagctttgcg
     6361 cggatgcaag atgtgcatga acccgtctga gtgcgctgac gtgtcgctcg cgtgccatat
     6421 acatgccttc tgtggccgac tgccagcccg ccaccgccag caactcgtgg cgcaacttgt
     6481 ccaggccaag accggttttg gctgacagca gcaggccgct tgcaagtgct gacggcgccg
     6541 ctgcatcagc cttgttccag acctcgatca caaccacatg ttgaggcagt tttttgctga
     6601 tagcccttat gatatctgcg tcggcagcta tgtattgagg agcgttttga cgggtcaggt
     6661 cgtgcaggaa cagcactgca tccgcgtgcg caatcgcgtc ccaggcgcgc gcgataccga
     6721 ttttttcaac ctcgtcatcg ctgtcacgca aaccagccgt gtcaatcaca tgcagcggta
     6781 caccttcaat ctggatggtt tgctgcactc tgtcacgggt agtgcccgcc accggcgtga
     6841 cgatggccag ttcggccccg gccagggcgt tgagcagtga tgacttgcca gcattgggtt
     6901 gacccgcaat cacgacttta atgccttcgc gcaaaagcgc accctgacgc gccttttgca
     6961 tcaccgtgtt cagcgtttgc tgtaaacgca acaactgccc agccgcgttg gtctgatgca
     7021 ggaaatcaat ttcctcttca ggaaaatcca acgtggcttc gatctgcacg cgcaaatgca
     7081 ccagtgcatc gcgcaaccgg tggatttcct gagagaaagc ccccgacagc gaacggctgg
     7141 cactgcgagc ggcggcctcg gtactggcat caatcagatc ggcaattgcc tcggcttgtg
     7201 ccaaatcaat cttgtcattg agataagccc gctcggaaaa ctcacccggc atggccagac
     7261 gcaagctggc caaatggggc gcgccatcgg ctgcggcctg cgccgcagcc tccaggcagc
     7321 gtgccaacag caattgcagt accaccgggc cgccatggcc ttgcagttcc agcacgtctt
     7381 cgccagtgaa tgagtgcggt gccgcaaaaa aaatggccag cccatggtca atcgcgacgc
     7441 cgtccttgtc cagaaacgcc gtataagtgg cctcgcgggc tttgagcgtg cgacgacaca
     7501 atgcccagat cagcccggca agcccgcgcc cgctgacccg cacaatgccc acagcaccac
     7561 gtccaggcgc agttgcgaca gcggcaatcg ggtcttgatg gcgcggcaaa ctcatgaccg
     7621 aaccgaccgt tcaacaggtg ctttgtggct gccggtgcgt tggagtgcag ctcggttcag
     7681 aaactcaact tgatgcattt tgtgcctttg ggagccggtc ggacttatgg cgcatcggcg
     7741 gtggaccaac ctcagcagcc cattttgcac tggctccttg atccagaact gggctattgg
     7801 cgggcaaatg gggcacgcac tggccgcgct ggggctgaaa ctcgcttttt ttttgatgcc
     7861 ataggtccga caaactcctg accctttagc cagtcacacc aaaagcgaca tgatagggcg
     7921 cattgtcggg gcagcatggg actgattatg cgtgcctgac cacagtggcg ggcgggcaaa
     7981 aaatgcttca tttcaggagt ttgattgtcc aactcgttag atcggcgatg aagggcttga
     8041 cggcccgttt ggtgtcagat tgccgggcta tttccagata gaatttaggg aaatagggga
     8101 aacggcctca aaactacaac attgagagtg aaagtgcgac catgaaagta ctgttaatta
     8161 cccgcgaata cccaccctac gtttatggtg gggctggtgt tcatgtcgaa tatctggcgg
     8221 ctgagctggc caaacgcatg cacgttgaag tccgctgctt tggcgaccag gacaccgtca
     8281 aagagggtgt acgggtcaaa ggattcccgt ttggcaaggg cagtttttcg cacgtggaca
     8341 agcgcctcag cggcgctctt gacaccttcg agaccaatct ggaaaccttg agcgagcaaa
     8401 ttgatgccga cctggtgcat gtgcacactt ggtacgcgca ccttggcggc atcctggcca
     8461 aaacgctgta cggtattccg ttggtccaca ccgtgcactc tctggagccg ctgcgcccct
     8521 ggaagcgcga gcagcttggc tgtggctatg acatgtcttc gtggattgag cgcacctcgc
     8581 ttggtctggc cgatgcggtg atcgctgtgt cagaaggcac caaggccgac atcctggagc
     8641 attttgatat cgacccggcc aaaatcacgg tcatttacaa cggcatcaag gtggaccagt
     8701 atcacccgtc cgaggacacc gatgcgctgg tgaaatacgg cgttgatccg gagcggcgca
     8761 cggtgctgtt tgttggccgg atcacgcgtc agaagggcat tctgcacttg gtgaatgcgg
     8821 tcaaatacat caatccggat gtgcagatcg tggatatctc cccgaacgac cgccgcgact
     8881 actg
//
LOCUS       DAOSLL010000126         8765 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7531_length_8765_cov_7.103559, whole genome shotgun sequence.
ACCESSION   DAOSLL010000126 DAOSLL010000000
VERSION     DAOSLL010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8765
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7531_length_8765_cov_7.103559"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(255..878)
                     /locus_tag="PLB25_07375"
     CDS             complement(255..878)
                     /locus_tag="PLB25_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HOE41436.1"
                     /translation="MSNLCHSPAAKQNRQPILEVLRQVLPATGTALEIASGTGQHVVW
                     FAAHLPGWTWQPSDVNPAALPSIAGWVAQQALTNVRPPVQLDVMAERWLADEAQATPA
                     QFDAIYCANMLHISPWATSAALMQGSARHMARGGRLITYGPYLEDEVATAASNLSFDA
                     SLRARNPDWGIRHRADLEQLAAQAGLRLAARHAMPVNNLLLVWSHLN"
     gene            complement(931..2394)
                     /gene="pmbA"
                     /locus_tag="PLB25_07380"
     CDS             complement(931..2394)
                     /gene="pmbA"
                     /locus_tag="PLB25_07380"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HOE41437.1"
                     /translation="MKKPNMPNNTAALPSAAATRPTARPAPSASSSATLADKGFAYSR
                     GFFEDLVDSALQHAKKLGASDAAAHASEGCGLSVSVRKGELENVERNRDKSLGITVHI
                     GQRRGNASTSDFSQQAIEQTAQAAFDIARFTAEDPFAALPDAADVAQEAEQRTDLELF
                     FPWTITSEDAARLAMVAEAAALKVDKRITNSEGAGVSAQQSHFFSAHTHGFRGGYASS
                     RHSVSVSPIAGKGDDMQRDAWYSSQRDAADLAAPEAIGRYAAERALSRLKARKIATTE
                     CAVLFESPLAAGLLGSFVQAISGGALYRKSTFLLDSLGKPVFPKHIDIAEDPFVRRGK
                     GSSPFDDEGVRVQARSVVEAGKVQGYFLGSYSARKLGLKTTGNAGGSHNLTLTSRLTQ
                     PGDDLEAMLQRLGNGLFVTELMGSGVNYVTGDYSRGASGFWVENGRIAYPVHEITIAS
                     NMKAMLKGIVAVGADSYNYGAKTVGSILIKRMKVAGS"
     gene            2418..3071
                     /gene="yjgA"
                     /locus_tag="PLB25_07385"
     CDS             2418..3071
                     /gene="yjgA"
                     /locus_tag="PLB25_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HOE41438.1"
                     /translation="MSRKLKKGYFVRGEFVAEGSERDLELKAELKGTTEQSRTDLKRE
                     STELQKLGSDLLDLRADLLERLALSDKLKDAIADAKRITNFEGKRRQMQFIGKLMRLL
                     EPELLGDVKKALDEQANGSAADNLVLHQAELWRERLLADDAAAAQWIMQHPGTDTQQL
                     RALIRQARKDAVPEKAGAAVRHGRAYRDIFQLVREALTAIPDNDVPAATFNIAREAS"
     gene            3068..3670
                     /gene="mog"
                     /locus_tag="PLB25_07390"
     CDS             3068..3670
                     /gene="mog"
                     /locus_tag="PLB25_07390"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HOE41439.1"
                     /translation="MNQPGNPFEAVKIGIVSISDRASSGVYIDKGLPALQDWLTRALK
                     NPLQFEPRLIADEQASISATLIELADAACALILTTGGTGPALRDVTPEATLAVAHKTM
                     PGFGEQMRQISLAFVPTAILSRQVAVIRGQSLIINLPGQPKAIAETLEGLKDAQGQQL
                     VEGIFAAVPYCIDLIGGPYLETDAAVCKAFRPKTAIRTFS"
     gene            4311..4442
                     /locus_tag="PLB25_07395"
     CDS             4311..4442
                     /locus_tag="PLB25_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41440.1"
                     /translation="MRAGDAAAAQLARLNLIGRGLLARAAAPVDDLDVVAAINEGRD"
     gene            4561..4836
                     /locus_tag="PLB25_07400"
     CDS             4561..4836
                     /locus_tag="PLB25_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOE41441.1"
                     /translation="MFFEFSNVMRRALLAKKITEARCRESLQLQQGLALHADAGDPSE
                     DLALRYQLSSYDAACHDLALRMRLPVATADGALRDAAVTCGVGVFVP"
     gene            complement(4982..5488)
                     /locus_tag="PLB25_07405"
     CDS             complement(4982..5488)
                     /locus_tag="PLB25_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE41442.1"
                     /translation="MSPAHEPTRKLVATDGIDAFDCGQPALNQFLQRYALVNQKANSA
                     QTYVCCQSGEVTGFYSLAVGSVDADLAPNRVTKGLARHPVPVMILARLAVDAQHQGQG
                     LGRALLKDALLRTARAADIAGIRCLLVHAKDDAARQWYLSWEFEPSPSDPYHLFLLIK
                     DLKSLLAD"
     gene            complement(5485..5763)
                     /locus_tag="PLB25_07410"
     CDS             complement(5485..5763)
                     /locus_tag="PLB25_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013122097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HOE41443.1"
                     /translation="MSTLNPSKSERIDVRASTSVKLLLQEAARACHKNVSEFLLDAGV
                     VAANQTLADRRQFVLDEAQWLAFQAALDAPTQAKLRLKQLLIEPGVLD"
     gene            complement(6070..7812)
                     /locus_tag="PLB25_07415"
     CDS             complement(6070..7812)
                     /locus_tag="PLB25_07415"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41444.1"
                     /translation="MYARFFRWASDRLNENGVLAFVTNRSFIESRTFDGFRKTVAQEF
                     ADIYVVDLGGDVRANPKLSGTKHNVFGIQTGVAISFMVKRVGTTKDKKPARVFYVRRP
                     ELETAEEKLGFLGKHPMRHLDFDEVSPDKSANWVNLTSNDFETLIPLASKESKAAKTA
                     AGERAIFKLFSLGVVTARDDWVFDYDRSDLATKAEFLIDTYNRDLIRLEAVRGSSKLE
                     EMLDSSIKWTRAVKNDLRRGTVYEYDDSQIIEASYRPFTKANLYFDKHLNEMQYQLGA
                     MFHGDANPTITFLCVSSSNALAAMAVEQPFDYCLLKMGNGGTQAVSRWVYDNSGNRVD
                     NITDWALEQFRAHYQPVVSAQAGNQSSATILKTAKAKTLDSRLLGNDGIEQRGNDGVG
                     RRANNAASITKEAVFHYCYAVLHDPIYREKYAQNLKREFPRIPFYPDFWQWAAWGEAL
                     MTLHIGYESVSPFPLTRSDEPDEKARIAGLSPKALLRSDAASGSIVLDSETTLRGIPS
                     EAWVYKLGNRCAIDWVLDQYKEKKPKDPTIREKFDTYRFADYKEKVIDLLMRVTTVSV
                     ETVRLVEAMKAVAR"
     gene            complement(7773..>8765)
                     /locus_tag="PLB25_07420"
     CDS             complement(7773..>8765)
                     /locus_tag="PLB25_07420"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOE41445.1"
                     /translation="NPSFRLAEEKFLSHAQEAINPSLTEADVREMLIQHILTEEIFAK
                     VFDDADFHQHNNVARELYALEGAFFTGALKKQTLKGLETYYAAIRAAAAQISSHSEKQ
                     TFLKIIYENFYKVYNTKAADRLGVVYTPNEIVRFMIDGADWLCEKHFGKNLIDRDVDI
                     LDPATGTGTFICELLEHFRGQPKKLAHKYQHELHANEVAILPYYVANLNIEATYAALT
                     GQYAEFPSLCFVDTLDNVGLHTSAKGVNADLFGSVSEENVARIKRQNSKRISVVIGNP
                     PYNANQANENDNNKNREYPQIDARIKQTYIAQSTAQKPSSTTCMRAFSAGPATG"
BASE COUNT         1678 a         2599 c         2607 g         1881 t
ORIGIN      
        1 cgcgacgaat gacttggaat gtttgaattc ttcgcatgat atgcaaagac ttgacctgtt
       61 tgagaccaat cattgatgtc cttgtcatcc cgccttgcct tgccaggcat ttgccatgtc
      121 tgcggcaggt tggccagaca ccaatgatct ggcaatggca ttgcttgcca tttatgaatc
      181 aaattgccct acagcccccg tcaaatatgc gctggcagct attgttaata tagcacgttg
      241 gaatcaacgc cggttcagtt cagatgcgac caaaccagca gcaggttgtt gaccggcatc
      301 gcgtggcgcg ccgccagccg caggccggcc tgggcggcga gttgttccag gtcggcgcgg
      361 tggcgaatgc cccagtccgg gttgcgcgca cgcagactgg catcaaagct caggttgctg
      421 gcagcggttg ccacttcgtc ttccaggtag gggccgtagg tgatcagccg accgcctcgg
      481 gccatatggc gggcgctgcc ctgcatcagc gcggcgctgg tggcccaggg cgaaatgtgc
      541 agcatgttgg cgcagtaaat ggcatcaaac tgtgctggag tggcctgcgc ctcgtctgcc
      601 aaccagcgct cggccatcac atcaagctgc actggcgggc gcacattggt caacgcctgc
      661 tgggcaaccc agccagcgat ggacggcagc gcagccgggt tgacatcgct gggttgccag
      721 gtccagcccg gcaggtgggc ggcaaaccag accacatgct gaccggtgcc agaggctatt
      781 tcgagcgccg tgccagttgc gggcagcacc tggcgcaaga cctcaaggat cggttggcgg
      841 ttttgcttgg cagcagggct gtggcacaga ttgctcatgg ccgcagcagc gccggtgttg
      901 cgccgccagc ctgatcaaag cccgctgcca tcagcttcca gccaccttca tgcgcttgat
      961 caggatcgag ccgaccgttt tggcaccgta gttatagctg tcagcgccca ccgccacgat
     1021 gcccttgagc atggctttca tgttgctggc gatggtgatc tcgtgaaccg ggtaggcaat
     1081 gcgcccgttt tccacccaga agccactggc accgcgcgaa taatcgccgg tcacgtaatt
     1141 gacaccgctg cccatcagtt cggtgacaaa cagcccgttg cccagccgct gcagcatcgc
     1201 ctccagatcg tcaccaggct gcgtcaggcg cgaggtcagt gtcaagttgt gcgagccgcc
     1261 cgcgttgccg gtggtcttga gccccagttt gcgggccgag tagctgccca gaaaatagcc
     1321 ctgcaccttg cccgcttcaa ccacgctgcg cgcctgcacc cgcacccctt cgtcgtcaaa
     1381 cggcgaactg cccttgccgc gcctgacaaa cgggtcttcg gcgatgtcaa tgtgcttggg
     1441 aaacaccggc ttgcccagcg aatcgagcaa aaaggtgctt ttgcggtaca gcgcgccacc
     1501 gctgatggcc tgcacaaagc tgcccagcag gccagccgcc aagggcgact cgaacagcac
     1561 tgcgcattcg gtggtggcga tcttgcgtgc tttcaggcgg cttaaggcgc gctcggcagc
     1621 gtagcggcca attgcctctg gcgcggccag gtcggcagcg tcgcgctgcg agctgtacca
     1681 ggcatcacgc tgcatgtcgt cacccttgcc ggcaatcggc gacaccgaca ccgagtggcg
     1741 cgaactggca taaccaccac gaaacccgtg cgtgtgcgcg ctgaaaaagt gcgattgctg
     1801 ggccgacaca cccgcgcctt cgctgttggt gatgcgcttg tcaaccttca gcgcggcggc
     1861 ctcggccacc atcgccaatc gcgcggcatc ctcactggtg atggtccacg gaaaaaacag
     1921 ctccagatca gtgcgctgtt cagcctcttg cgccacgtca gcagcatcgg gcaaggcggc
     1981 aaacgggtct tcggcggtga agcgggcaat gtcaaacgca gcctgcgcgg tttgctcaat
     2041 cgcctgctgc gaaaagtcgg aggtgctggc gttgccgcgg cgctgcccga tgtgcaccgt
     2101 gatgccaagc gacttgtcgc ggttgcgctc gacgttttcc agctcgccct tgcgcaccga
     2161 cacgctcagg ccgcagccct cggacgcatg tgctgcggca tcgctggcac ccagtttttt
     2221 ggcgtgctgc agcgcgctgt ccaccaggtc ctcaaaaaag ccccggctgt aggcaaagcc
     2281 cttgtcagcc agcgttgcgc tgctgctggc agatggcgca ggccttgcgg tgggtcgtgt
     2341 ggcagcagca gaaggcaacg cagcggtgtt gttgggcatg tttggttttt tcatagcggc
     2401 ggctatgata cttggctatg tcaagaaaac tgaaaaaggg ctactttgtc cgcggtgagt
     2461 ttgtcgccga gggcagcgaa cgcgaccttg agcttaaagc cgagctaaag ggcaccaccg
     2521 aacaaagccg caccgacctc aaacgcgaaa gcaccgaact gcaaaaactg ggcagtgatc
     2581 tgcttgatct gcgcgccgac ctgctggagc ggctggccct gtccgacaag ctcaaggatg
     2641 ccatcgccga tgccaagcgc atcaccaact tcgagggcaa gcgccgccag atgcagttca
     2701 ttggcaaact gatgcgcctg ctggagccag agctgcttgg cgatgtcaaa aaggcactgg
     2761 atgagcaggc caatggctca gccgccgaca acctggtcct gcatcaggcc gagctttggc
     2821 gcgagcgcct gcttgctgac gatgcggccg ctgcgcagtg gatcatgcaa caccccggca
     2881 ccgacaccca gcaactgcgc gccctgatac gccaggcgcg caaggacgcg gtgccagaaa
     2941 aagctggcgc ggcagtgcgc catggccgcg cgtaccggga catctttcaa ctggtgcgcg
     3001 aggcgctgac cgccatccct gacaacgacg tgcccgccgc cactttcaac attgcccggg
     3061 aagcctcgtg aaccagcccg gcaacccctt tgaagcggtc aagattggca ttgtgtccat
     3121 cagcgaccgc gcctccagcg gcgtttacat cgacaagggc ttgccggcgc tgcaggactg
     3181 gctgaccagg gcgctgaaaa atccgctgca gtttgagcca cgcctgattg ccgatgaaca
     3241 ggccagcatc agcgcgaccc tgattgaact ggctgatgcc gcttgtgcgc tgattttgac
     3301 caccggcggc accggcccgg ccctgcgcga cgtgaccccc gaagccacgc tggcagtggc
     3361 gcacaagacc atgccgggct ttggcgagca gatgcgccag atcagtctgg catttgtgcc
     3421 caccgccatc ttgtcgcgcc aggtggcggt gattcgcggg caaagcctga tcatcaacct
     3481 gccgggccag cccaaagcca ttgccgaaac gcttgaaggc ctgaaggatg cacaggggca
     3541 gcaactggtc gaaggcatct ttgcggcagt gccgtactgc atcgacctga ttggcgggcc
     3601 gtatctggag accgatgcgg cggtgtgcaa ggcattccgg ccaaagaccg cgattcgcac
     3661 gttttcataa gccaaatcgg gctctagccc ttatggatag tgcgctggca gctatctatt
     3721 ttgatgttgc tggttgattg ggtttgggct tgggtttggg tttgggtttg ggtttgggct
     3781 atttgggggt gaattggcgc atcacttggc tggtgtgggc ggtggactga gcccggccca
     3841 gcagccgaca ggggtggccg cctcatgctg gcgtaccaga aatcggcggc cacccctgtc
     3901 gaccgctggg cctgccggtt gtgttggctg gcggcttggt ggtcaaccaa atgcatcgtc
     3961 actcaatgga tgtctgctgc atgctgcctt caatctggct agccaactca gttaaaggcc
     4021 agaagcatgg aaaccatcgc agtctttgag gatgtggtca gaaataactt ccccatttgc
     4081 accggcggat ttgggcgcac agcgtcgttg caagacgctt gtttagccag ctaaacggcg
     4141 cgtcttgcgc ctagccgtgc atcccaactc cttggtgcaa aacggggagt tattcccgac
     4201 cacatcctga ggcgaaatcc agtctgtctg acattctcgc cgccgtcgaa ctcggtcagg
     4261 aattcacggt caccaagcgg ggcgcgccga ttgccaggat cattccttac ttgcgtgcag
     4321 gcgacgctgc tgcggcacag ttggcgcggc tgaacctgat tggccgaggc ctgttggccc
     4381 gcgctgcggc cccggttgac gatcttgacg tggtggccgc gatcaacgag gggcgtgatt
     4441 gatgcctttt gtgatcgaca actcggttgt catcggctgg catctcaata ggtgggccac
     4501 ggcttattcg gacgcggtgt tgagggcgct ggtcaacgac aacgcgcatg ttccggcgtt
     4561 atgttttttg agttttccaa cgtcatgcgc cgggcattgc ttgcaaagaa gatcacagaa
     4621 gcgcggtgca gagagagttt gcagttgcag caggggctgg cgcttcatgc cgatgcaggt
     4681 gatccgtcag aggacctggc cctgcgttac caattgagta gctatgacgc tgcctgccat
     4741 gacctggcct tgcgtatgcg cctgccggtg gcgacagcag atggcgcgct acgggatgct
     4801 gcagtgactt gcggcgttgg cgtgttcgtg ccttgagccc caagttgccc gggtatgccg
     4861 ccgtgctggg gcacctggtc tgtgatctgt gcatgcagat tttcctgact gtgcgcgact
     4921 ggccggtatc aatggcctgc ggcaccggcc tggtccagtt tcaagcagat gaccgatcaa
     4981 atcaatccgc cagcaaactc ttgaggtctt tgatcagcag aaacaagtgg taggggtcag
     5041 acgggctggg ctcgaactcc caggacagat accactgccg cgcagcgtcg tccttggcgt
     5101 gcaccagcag gcagcgaata ccggcaatgt ctgcagcgcg cgccgtgcga agtagcgcat
     5161 ccttcaaaag cgcccgaccc agcccctggc cctggtgctg cgcatccacc gccaatctgg
     5221 ccagaatcat caccggcacc gggtgacgcg ccagcccttt ggtcacccgg tttggcgcca
     5281 gatcagcatc aacgctgccc accgccaaac tgtaaaagcc cgtcacctcg cccgattggc
     5341 agcaaacata agtttgtgca ctgttggcct tttgattaac cagcgcatac cgttgcaaga
     5401 actgattcag ggcgggctgc ccacaatcaa acgcatcaat gccgtcggta gcgaccagtt
     5461 tgcgagttgg ctcgtgagct ggactcaatc cagtaccccc ggctcaatca ggagctgttt
     5521 cagccgcagt ttagcttggg ttggcgcatc cagcgccgct tgaaacgcta gccattgcgc
     5581 ctcgtcgagc acaaactgcc gacgatccgc caaggtttga ttggccgcca ccacaccggc
     5641 atcgagcaaa aattcgctca catttttgtg gcaggcgcgc gcggcttctt gcaacagcag
     5701 cttgaccgat gtgctggcgc ggacatcaat ccgttcggat ttggatgggt tcaaggtgct
     5761 catggcggct ttctttgagg gttggcatgc atcatagcgt ccggatgatg tcctgacaag
     5821 acggcgttca ttggcggcat gtaccgatca acacttccga acgatgggtt tcagtaaacc
     5881 cagcggctcg gtacgcaagg cccgcagttc agcgccgagg gccacaggat cagccacatc
     5941 cagcacgccc agaattaaag taccgcgaac acctctcttg acctggcagc gccctccctt
     6001 tgccatcgaa cagcgctggg tctcctgcct cgtccccgtc gtattgccgc ccgggcggcg
     6061 gcgcttcgct caccgcgcca ccgccttcat ggcctcgacc agcctgaccg tctcaacgct
     6121 gacggtggtc actcgcatca gcaggtcgat caccttttcc ttgtaatcag caaaacggta
     6181 ggtgtcgaac ttctcgcgga tagtcgggtc tttgggtttt ttctctttgt actggtccag
     6241 cacccagtcg atggcgcagc ggttgcccag cttgtaaacc caggcctcgc tcggaatgcc
     6301 gcgcagcgtg gtctcagaat caagcacgat gctgccagag gcagcgtcag agcgcagcaa
     6361 ggctttgggc gaaagcccgg cgatgcgggc cttttcgtca ggctcgtcgc ttcgggtcag
     6421 cgggaacggc gacaccgact cataaccgat gtgcagcgtc atcagcgcct caccccaagc
     6481 ggcccattgc cagaagtccg ggtaaaacgg gatgcgtgga aactcgcgtt tcaggttttg
     6541 cgcgtatttt tcgcggtaga tggggtcatg cagcacggca tagcagtagt ggaagacggc
     6601 ttccttggtg atgctggctg cgttattggc gcggcggcca acgccgtcat tcccgcgctg
     6661 ctcgatgccg tcatttccga gcaggcggga atccagtgtt ttagctttcg ctgtcttcag
     6721 gatggttgcc gacgactggt ttcccgcctg agccgaaacg acgggctgat agtgggctct
     6781 gaattgttca agtgcccagt cggtgatgtt gtcgacacgg ttgccggagt tgtcgtatac
     6841 ccaccgcgaa acagcctgcg ttcccccgtt tcccattttc agcaggcaat agtcaaacgg
     6901 ttgctcaaca gccatcgccg caagagcatt tgacgatgac acgcaaagga aagtgatagt
     6961 tgggttcgcg tcgccgtgaa acattgcacc aagctgatac tgcatttcat tgagatgctt
     7021 gtcaaaatac agattcgctt ttgtaaacgg ccgatatgac gcttcaataa tctgactatc
     7081 gtcgtattcg tacactgtcc ctcgccgaag atcattcttg acggctcgcg tccacttaat
     7141 tgatgagtcg agcatttcct ctaatttgct gctcccacga accgcctcta atcgaatcaa
     7201 gtcgcggtta tatgtgtcga tcagaaattc agcttttgta gccaagtcgc ttcggtcata
     7261 gtcaaaaacc cagtcatcac gtgcggtaac gacaccaagc gaaaacagct tgaagatcgc
     7321 ccgctcacca gccgccgtct tcgccgcctt ggactccttg ctcgccagcg ggatcagcgt
     7381 ctcaaaatcg ttgctggtca gattcaccca gttggcgctt ttgtccggcg acacctcgtc
     7441 aaagtccagg tggcgcatcg ggtgtttgcc caaaaacccg agcttttcct cggcagtttc
     7501 cagctccggg cggcgcacgt aaaaaacgcg ggccggtttt ttgtctttgg tggtgcccac
     7561 ccgtttgacc atgaaactga tggcaacccc ggtctggata ccaaacacat tgtgtttggt
     7621 gccactgagt ttggggttgg cgcgcacgtc gccgcccagg tccaccacgt aaatgtcggc
     7681 aaactcctgt gccacggtct tgcgaaaacc gtcaaacgtg cggctctcaa taaaactgcg
     7741 gttggtcaca aacgccagca ccccgttttc gttcagccgg tcgctggccc agcggaaaaa
     7801 gcgcgcatac atgtcgtaga gcttggtttt tgcgcggtgc tctgggcaat gtaggtttgc
     7861 ttgatgcgcg cgtcaatctg cgggtattcg cggtttttgt tgttgtcgtt ctcattggcc
     7921 tggttggcgt tgtaaggcgg gttgccaatc acgacgctga tgcgcttgct gttttgcctc
     7981 ttgatacggg ccacgttttc ttccgagacg ctgccaaaca gatcggcatt gacacccttg
     8041 gccgacgtat gcaggcccac gttgtccagc gtatcgacaa agcacaggct gggaaactct
     8101 gcgtactgcc cggtcagcgc ggcgtaggtg gcctcgatgt tcaggttggc cacgtaatac
     8161 ggcaggatgg ccacctcgtt ggcgtgcagc tcgtgttggt atttgtgcgc cagtttcttt
     8221 ggctggccgc gaaagtgctc cagcagttcg caaatgaagg tgccggtacc ggtggccggg
     8281 tccagaatgt ccacatccct gtcaatcagg tttttgccaa agtgcttttc acacagccaa
     8341 tctgccccgt cgatcatgaa acgcacgatt tcgttgggcg tatagaccac gcccagccgg
     8401 tcagcggcct tggtgttgta gaccttgtaa aaattctcgt aaatgatctt caagaaggtc
     8461 tgcttttcgc tgtggctgct gatttgcgcg gcggcggcgc gaatcgcggc gtaataggtt
     8521 tccaggcctt tgagggtttg ctttttcaac gccccggtga aaaaggcgcc ttcgagcgca
     8581 tacagctcac gcgccacgtt gttgtgctgg tgaaagtcgg catcgtcaaa caccttggca
     8641 aagatttctt cggtgaggat gtgctggatc agcatttcgc gcacatcggc ttcggtcagc
     8701 gacgggttga tggcctcttg cgcgtggctc aggaattttt cttcggccag gcgaaagctg
     8761 gggtt
//
LOCUS       DAOSLL010000127         8753 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7540_length_8753_cov_5.034836, whole genome shotgun sequence.
ACCESSION   DAOSLL010000127 DAOSLL010000000
VERSION     DAOSLL010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8753)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8753)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8753
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7540_length_8753_cov_5.034836"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="PLB25_07425"
     CDS             complement(<1..288)
                     /locus_tag="PLB25_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HOE41446.1"
                     /translation="MSLDLDDRQRAMLAEMGVRVWLPLKPEAGPLAADAADLQVTIKK
                     EAASAYAARAGGHFPVNLPGLTGATEVPTAPDPARSAIVSGSASPVTPGTAG"
     gene            complement(307..783)
                     /gene="rimI"
                     /locus_tag="PLB25_07430"
     CDS             complement(307..783)
                     /gene="rimI"
                     /locus_tag="PLB25_07430"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HOE41447.1"
                     /translation="MNARLQAAEARFEPMTAAALDSVLAVELQAYAHPWLASHFADCL
                     NSGYQAQLLLANDTLLGYFVAMKGFEEAHLLNITVSPSYQRQGWSQVMLDALALWARG
                     QGARWLWLEVRPSNTRAIHVYKVHGFRYVGRRKQYYPASHGQREDALVMSCKLDSP"
     gene            complement(780..1550)
                     /gene="tsaB"
                     /locus_tag="PLB25_07435"
     CDS             complement(780..1550)
                     /gene="tsaB"
                     /locus_tag="PLB25_07435"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HOE41448.1"
                     /translation="MTRFIEANASQLEAPLLNLLAFDTSTERMSIAVQRRGQAQPWLY
                     QSAGGAQTSTQLIPQIQALMGQAELRFGQLDAIVFGAGPGSFTGVRTACSVAQGLGFG
                     AGVALLPVDTLLAVAEDARDQLNASTGLVVTALLDARMAEMYANSYILESGVWLPDKG
                     CSVIKPEHLAFSPPPGAALWVLAGNVFEAYGARLPSCPAQRINAAPTAAALLRLAPRL
                     LAVGAALPAALALPTYVRDKVAQTTLERAALKSDLLQP"
     gene            complement(1779..3420)
                     /locus_tag="PLB25_07440"
                     /pseudo
     CDS             complement(1779..3420)
                     /locus_tag="PLB25_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
     gene            3953..5452
                     /gene="dacB"
                     /locus_tag="PLB25_07445"
     CDS             3953..5452
                     /gene="dacB"
                     /locus_tag="PLB25_07445"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HOE41449.1"
                     /translation="MTQHEWGWSSLPESAGLQRARLAWALAGALLGATPVLAQALPAA
                     VSSAFARAQIPLDAVAMLVADADGKTVPRLSHRADVAMNPASVMKLVTTFAALDLLGP
                     AYVWRTPVWLDGRVQDGVLRGNLVIQGQGDPKLVQERLWLLLKRLQGLGIQTIDGDMV
                     LDRRSFAQPESSPADFDGEPLRPYNVAPDALLFNFKSVSMTFMPDLLAGVVRVSYDAP
                     LWGVQMPASVPLSTVKNAACGDYRTGLKADFGDPARLTFAGNYPAACGEKTWPVAYVD
                     PVSYNARNLEGLWRSMGGQLVGRVRSGPAPTSAATFELVSPPLAEVIRDINKFSNNVM
                     AQQVFLTLALPRPNPADSAGSTPFTPVTRDMAREVVRRWWQQRISVDGVPVLDNGSGL
                     SRLERISATGLLRLLQVAYASPAMPELMSSLPIVGIDGTLKRSRATAASGHLKTGSLR
                     DVTAIAGYVLTASGKRLCLVAMVNHTNAPAARPALDAFLQWAVGDNAAL"
     gene            5488..5742
                     /locus_tag="PLB25_07450"
     CDS             5488..5742
                     /locus_tag="PLB25_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET transporter"
                     /protein_id="HOE41450.1"
                     /translation="MTFTDFIGTLAAVLTTGSFLPQAWHTFTTKDVSGISLGMYSVFT
                     TGVAGWLLYGLLLQAWPIVIANAITLALASAILAMKLRYR"
     gene            complement(5795..6733)
                     /gene="rsmI"
                     /locus_tag="PLB25_07455"
     CDS             complement(5795..6733)
                     /gene="rsmI"
                     /locus_tag="PLB25_07455"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HOE41451.1"
                     /translation="MTTRDASLAASFATALSAASDAAGSQNYPPATLYVLATPIGNLA
                     DISLRALHLLQLADVIACEDSRHSQALLRAYGIHKPASQWLAVHQHNEAQAVQTVLDR
                     LNQGQRVVYVSDAGTPAISDPGARLVAGVRQADLPVVPVPGVSSVTAILSVAGVVADA
                     QQHGAFVFAGFLPSKAGERASAIAAFSTEMRPVVLLEAPHRIEALALALAVLGQRQLT
                     IGRELTKQFEEITTLAAAGLPDWLSQDANRTRGEFALVLHPPVLVPDRQPDTGVLRLL
                     LAELPLKTAVKLAAGITGAPRNVLYQQALAIKAEQA"
     gene            6736..7128
                     /locus_tag="PLB25_07460"
     CDS             6736..7128
                     /locus_tag="PLB25_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HOE41452.1"
                     /translation="MVFPETKVRLKPRTTKQSGDASEALALAHLQRAGLVLLERNYRT
                     PGRGGGEIDLIMRAPDGTCVFVEVRQRKMASHGGAAASVSLTKRHRIVYAARHYLLRF
                     ASLPPCRFDVVAVDAGTLEWLQGAFDAG"
     gene            7394..7993
                     /locus_tag="PLB25_07465"
     CDS             7394..7993
                     /locus_tag="PLB25_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HOE41453.1"
                     /translation="MLEQRIQQHFIDSADLKYQAAPVLSKPIADAVQALLACVTSGGK
                     VLVCGNGGSAADAQHFAAEFVGRFERERPELAAIALTTDSSILTAVANDYGFDIVFSR
                     QVRALGQAGDVLLALSTSGNSGNVLKAIEAAHEREMVVIGLSGRGGGKMLQALRDTDV
                     HVCVPHDRTARIQEVHLLTLHCLCDAVDTLLLGDLENPP"
     gene            7990..8631
                     /locus_tag="PLB25_07470"
     CDS             7990..8631
                     /locus_tag="PLB25_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOE41454.1"
                     /translation="MKHTTQHLFTLLALTASLGGILTACVPVIIGGAVVGTAMVASDR
                     RTSGAQLEDEGIELRAASRLQEALGDRLHVNVTSYNRRVLLTGEVPAAQDKQSAEQIV
                     SKVDNVESVVNELMVAGNSNISDRSSDALITGKVKAGLVDAKDLFANAFKVVTERGAV
                     YMMGRVTQREADRATEVARSTSGVKKVVRVFEFISEDELRGMMPKPAPGAAKN"
     gene            complement(8650..>8753)
                     /locus_tag="PLB25_07475"
     CDS             complement(8650..>8753)
                     /locus_tag="PLB25_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOE41455.1"
                     /translation="RLPVTALVDQFYADVQQLGGNRWDTSSLIKRLK"
BASE COUNT         1685 a         2673 c         2698 g         1697 t
ORIGIN      
        1 gccagcagtg cctggcgtga ccgggctggc actgccgctg acgatggcag accgtgccgg
       61 gtctggggcc gttggcactt cggtagcccc ggtcaagccc gggaggttaa caggaaaatg
      121 gccaccagcc cttgctgcat atgcgctggc agcttctttt tttatagtaa cttggagatc
      181 ggcagcgtcc gccgccagcg gcccggcctc aggcttcagt ggcaaccaga cgcgcacgcc
      241 catctcggcc agcatggcgc gttgacggtc atcgaggtcc agactcatgg gctgattctc
      301 accggttcag ggcgaatcga gcttgcagct catcaccagt gcgtcttcgc gttgcccgtg
      361 gctagccggg taatactgct tgcgtcgccc aacgtagcga aagccgtgca ccttgtaaac
      421 atggatggcg cgggtgttgc tcgggcgcac ttccagccaa agccagcggg caccctgacc
      481 acgcgcccac aacgccagtg cgtccagcat cacctgcgac caaccctggc gctggtagct
      541 cgggctgacg gtgatgttga gcagatgggc ctcctcaaag cccttcatgg cgacaaaata
      601 gcccagcagg gtgtcgttgg ccagcagcag ttgcgcctgg tagccgctgt tgaggcaatc
      661 ggcaaagtga cttgcaagcc aggggtgggc ataggcctgc aactccacgg ccagcacact
      721 gtccagcgcg gcagcggtca tcggctcaaa gcgcgcctcg gcggcttgca agcgtgcatt
      781 catggctgca gcaggtcaga tttgagggcc gcgcgctcga gggtggtttg cgccactttg
      841 tcacgcacat aggttggcag ggcaagtgcc gccggtagtg ccgcgccgac cgccaacagg
      901 cggggtgcca ggcgcagcag ggcagccgca gtcggtgcgg cgttgattcg ctgcgccgga
      961 caggagggca gacgcgcgcc atacgcctca aacacattgc ccgccagcac ccagagggca
     1021 gcaccgggtg gcggtgaaaa cgccagatgc tctggcttaa tcacactgca acccttgtca
     1081 ggcaaccaaa ctccgctctc caatatatag ctgttggcgt acatttcagc cattctggca
     1141 tccagcaagg cggtgacaac caggccggtg ctggcgttca attggtcgcg cgcgtcttcg
     1201 gcaacggcca gcagggtatc gaccggcagc agcgccaccc cggccccaaa acccagccct
     1261 tgcgcgaccg agcaggcggt ccgaacgccg gtaaacgagc ccggcccagc gccaaacaca
     1321 atcgcatcaa gctgcccaaa acgcaattcg gcctgcccca tcaaggcctg tatctgggga
     1381 atcagttggg ttgaggtctg cgccccacct gctgactggt acagccaggg ctgcgcctgc
     1441 ccccggcgct gcaccgcaat cgacatccgc tcggtgctgg tatcaaaggc cagcagattg
     1501 agcagtggcg cctcaagctg gctggcattg gcctcgatga acctggtcaa attagccccc
     1561 agcccttatg cagactgcgc ggtaagctat tgtaagtata gcaattgagg cggtctgacg
     1621 agtcccgcaa aacatcctgc ccaggacctg ctgcagaccc gcgcggcggc cttgcagcgc
     1681 aaatggcaat cgtggcggca aaccgttgaa aaaaaccgtt acgcgcttgg aacatggcct
     1741 tattatcctt ggacaccagc atcgccatgt gcagacactt acaccgtgac gtgaatctcg
     1801 tcgagctggc ttgggctcag gaactgctcg ccgtagcggc gatagacacc ctgtttgaca
     1861 aacacgtcaa acaaatccgg gtcaatgtgg ccaccttgtt tcatgtcgtt catgatggcc
     1921 atggcctgcg acagcttcat gctgggcttg tagggccggt ctgatgcggt cagggcctcg
     1981 aaaatatcgg caatgcccat gatgcgcgcc tgcaccgaca tctgctcgcg cgacaaaccc
     2041 ttggggtaac ccttgccgtc catgcgctca tggtgaccgc cggcgaactc aggcacgttt
     2101 ttcagatgct tgggccaggg cagccgttcc agcatcttga tggtcgcaac aatgtgatag
     2161 ttgatgatgt cgcgctcggc agtggtcagc gtgccctttc gaatgcacag gttgtccatt
     2221 tcatcggcag acaaaaagtc ggtgtgcacg ccattggcgt tgcgccactg gtactggctg
     2281 ccaatctggc gcacccggat gatgtcggca tctttcatgc cctcgccgcc cacattggtg
     2341 gtccgcaaga actcgcggtc tttgtcaagt gtctgctgtg cctccagtga ttgcgccagc
     2401 agcagtgctt cggcctccgg atcaatcggc gcacgcaatg ccagttgctg gcgcagcgcg
     2461 gcaatttcgg catcgcgctt aaggacttca aagcgggtgt caatcagctc aatgcggtcg
     2521 aacagggttt gcagcttggt ggccttgtcc accacgtgga ccggggtggt aactttgccg
     2581 cagtcatgca gcaatccggc aattttgagc tcgtaacggt ccgcctcgct catctgaaag
     2641 tcggccattg gcccgctctg cacgtcggcc acggcatcag cgagcatcat ggtcagcgct
     2701 ggcacccgct ggcaatgacc gccggtgtaa tgcgacttct cgtcaatggc cagattgatc
     2761 aggccaatga aggcctcaaa gagattttcg agctgggtca tcagcaaacg gttgctcaat
     2821 gcaatggcgg cctgggatgc cagcgactcg accaggcgct ggtcgggggg gggaaaacgc
     2881 cagcacctcg cgcgtgccag gctttaacgc gttgatcagt tgcagcacgc caatcacatc
     2941 gccctcatgg tcacgcatcg gaaccgtcaa aaacgacttt gatcggtaac cggtgcgctt
     3001 gtcaaacacc tgggtgccgg aaaagtcgaa ctcgtcggcg ctgttgtaag catcttcaat
     3061 gttgacggtg accccgtgaa tcgccgcata ggccgccacc agcgaatcgt tgggctggcc
     3121 gtcgctgcgt agcagtggaa tgtccgggaa attgattttg ctgcccgtgg taccgcccat
     3181 ggcaatgtcg agcgaatccg tgcgcatgat ctcaaaacgc agcgtgtgcc cgttgtcgag
     3241 catgcggtaa agcgtgccac cgtcagcatg ggtgatggtt ttggccgcca ccaggatctg
     3301 ctccagcaat tgattgatgt cgcgctgacg cgacagcgaa gcaccaacgt cgttgagatg
     3361 ctccaggcgc ccaagcaggc catgaacgga actcgattcg caggcggttg ttgcactcat
     3421 atggcccttc gtgattgagt ctgcacagtg tccgaacgga aagtcatttt atatataaaa
     3481 atcatatagt tagatgactt tcgccaaacc ggggctgctg caggacaacg gcattgatac
     3541 gccttcgaaa aaaccgtccc gaaagcagcc agtcttttga ccaatacctt caatttgcaa
     3601 ataatgtaac ttattgatac atatcaacga atcagggttt tattgcgaca ttgtcgagcc
     3661 acgacagatg gtaccgatag gcgggcagac aagtcgcaat gacccgccgc aaggcacacc
     3721 ggcagcggtt gtgatgccat ttccaaccca tccaagttgt tgttttctcg ccttgccttg
     3781 cgtttgcaag acttgcgcca gggtgcctga ccacgcaaga tctggcaatg atctggcaat
     3841 gcgatgcgcc agcagcgcgt gcgatgatgc ggtgcagatt gaacctgggt tgaacacagc
     3901 atcgctggtg ccgggcatgg gcctgccgcc agatcacaca aagggatgaa caatgacgca
     3961 gcatgagtgg gggtggtcga gtctgccaga gtccgctggg ttgcagcggg cgcggctcgc
     4021 ctgggcgctg gctggcgcac ttttgggggc cacgccagtg ttggcacagg ctttacctgc
     4081 agcggtgagc agcgcttttg cgcgagctca gattccgctg gatgcggtgg ccatgctggt
     4141 cgctgatgcc gacggcaaga ccgtgccgcg cctgagtcac cgcgccgatg tagcgatgaa
     4201 cccggcctcg gtcatgaagc tggtcaccac gtttgcggcg ctggacttgc ttggcccggc
     4261 ctatgtctgg cgcaccccgg tgtggctgga tggccgggtg caggacgggg tgctcagggg
     4321 caacctggtg attcagggtc agggcgaccc caagctggtg caagagcgcc tgtggctgtt
     4381 gctaaagcgt ctgcaaggct tgggcatcca gaccatcgac ggtgacatgg tgctggaccg
     4441 gcgcagcttt gcccaacctg aatcaagccc tgccgacttc gacggtgaac cgctgcgtcc
     4501 ctacaacgtg gcacccgacg cgctgctgtt caatttcaag tcggtgtcaa tgactttcat
     4561 gcctgatttg ctggccggtg tggtccgggt cagttacgac gcgcccttgt ggggcgtgca
     4621 gatgcccgcc agcgtgccgc tgtcaacggt caaaaatgcc gcctgtggtg attaccgcac
     4681 tgggctcaag gctgattttg gcgacccggc acggctgact tttgccggca actacccggc
     4741 ggcctgtggt gaaaaaacct ggccagtggc ctatgttgat ccagtcagct acaacgcccg
     4801 caacctcgag ggcttgtggc gcagcatggg cgggcagctt gtcgggcggg tgcgcagcgg
     4861 accggcaccg accagcgctg ccacatttga actggtcagc ccgccactcg ccgaggtgat
     4921 ccgcgacatc aacaagttca gcaacaacgt catggcacaa caggtttttt tgacgctcgc
     4981 gctgccccgg cccaacccgg ccgacagcgc tggcagcaca ccgttcacac cggtgactcg
     5041 cgacatggcg cgcgaggtgg tgcgacgttg gtggcaacaa cgcatcagcg ttgatggcgt
     5101 gccggtgctc gacaacggtt caggcctgtc acgcctggag cgcatcagcg ccacaggcct
     5161 gctcaggctg ctgcaagtgg cgtacgcgtc gcccgccatg cccgagctga tgtcttcgct
     5221 gcccatcgtc ggcatcgatg gcaccctcaa acgcagccgt gccacagcgg ccagcggaca
     5281 tctgaaaacc ggcagcctgc gcgatgtcac ggccatcgca ggttatgtgc tgacggccag
     5341 cggtaagcgc ctgtgcctgg tggccatggt caaccacacc aacgcgcccg ccgcccgccc
     5401 ggcgctggat gccttcctgc aatgggcggt cggcgacaat gccgctcttt gattccggca
     5461 cggcgtgccc gtcacgaccc caaccacatg actttcacag atttcatcgg caccttggcg
     5521 gccgtcctga ccaccggcag ttttttgcca caggcctggc ataccttcac caccaaggat
     5581 gtcagtggca tttcactggg catgtacagc gtctttacca ccggggtggc tggctggctg
     5641 ctgtacgggc tgctgctgca ggcctggccg attgtgatcg ccaacgcgat caccctggca
     5701 ctggccagcg ccattctggc gatgaagctg cgctaccgct agccaccttt gcggcaagcg
     5761 cccgacccaa gcgcaaagtg ggtcaagcga cggcttatgc ctgctcggcc ttgattgcca
     5821 gtgcctgttg gtaaagcaca ttgcgtggcg cgccggtgat cccggcagcc agtttgaccg
     5881 cagtcttcag cggtaactca gccagcagca gcctgagcac ccccgtgtcg ggctgccggt
     5941 ctggcaccag taccggcgga tgcaggacca acgcgaattc gccacgcgtg cggttggcat
     6001 cctgggacag ccagtccggc aggcctgcgg cagccagcgt ggtgatttct tcaaactgct
     6061 tggtcagttc gcggccaatc gtcagttgcc gttggcccaa taccgccagc gccagcgcca
     6121 gcgcttcgat gcgatgcggt gcctccagca acacgacagg gcgcatttca gtggaaaagg
     6181 ccgcgatggc actggcgcgc tcgccggcct tggacggcaa gaacccggca aacacgaaag
     6241 caccgtgttg ctgggcatcg gcgacgacgc ccgccacgct caatattgcg gtgacgctgc
     6301 tgaccccagg cactggcacc accggcaagt ctgcctgccg cacaccggcc accaggcgcg
     6361 cgccagggtc actgatggcc ggtgtaccgg catcgctgac atagaccacc cgctgcccct
     6421 ggttgaggcg gtccagcacc gtctgcaccg cctgcgcttc gttgtgctgg tgcaccgcca
     6481 gccattgtga ggctggcttg tgaatgccgt acgcgcgcag cagggcctgg ctatggcggc
     6541 tgtcctcgca ggcgatcaca tcggccagtt gcagcaaatg cagtgcccgc aggctgatgt
     6601 cggccagatt gccaatcggg gtggccagca catacagcgt cgcaggcgga taattctggc
     6661 tgcctgccgc gtcagatgcg gcactcaaag ctgtagcgaa acttgccgcc aaactggcat
     6721 cacgggttgt catcaatggt ttttcctgaa acaaaagtac ggctcaagcc acgtaccacc
     6781 aagcagtctg gcgatgccag cgaagcgctg gcgctggccc acctgcagcg tgccgggttg
     6841 gtcctgctgg agcgcaatta tcgaacccca gggcgtggtg gcggcgagat tgacctgatc
     6901 atgcgtgcac ctgacggcac ctgtgttttt gtcgaggtgc gccagcgcaa gatggccagc
     6961 cacggtggcg cagcggccag tgtcagtttg accaagcggc atcgcattgt gtatgccgca
     7021 cggcactatt tgttgcgctt cgccagcttg ccgccctgcc gttttgatgt cgtggcggtc
     7081 gatgcgggaa cgcttgagtg gctgcagggc gcatttgatg ccggttgacg aacccggttg
     7141 ccacatcggt atgccccggt tctgccagaa ggctgtcgga cggatgaatc gggctgccag
     7201 cacgaccccc ggcgaggcca gtttgcgccg gttttgcagg ccgatggccc agttgcgcgt
     7261 caagggccag gtgcacaatg ggccgttccc ggttcgcagc cgatgatgca caggttcgac
     7321 ggtctgccag gccggcaaat ggtcaaagac cagcccgggc gcgatcaggc ttgagacagg
     7381 tatcatcacc gccatgcttg agcaacgcat ccaacaacat ttcatcgaca gtgctgacct
     7441 gaaataccag gcggcaccgg tcttgagcaa acccattgcc gatgccgtgc aggcactgct
     7501 tgcctgtgtc accagtggcg gcaaggtact ggtctgtggc aatggcggtt cggccgccga
     7561 tgcccagcac tttgccgcag aatttgtcgg ccggtttgag cgcgagcgcc ccgaactggc
     7621 ggcgattgcc ctgaccaccg acagttcgat cctgaccgcc gttgccaacg actatggttt
     7681 tgacattgtt ttttcgcgcc aggtacgcgc gctgggacaa gcgggcgacg tgctgctggc
     7741 cctgtccacc agcggcaact caggcaatgt gctcaaagcc attgaggccg cgcatgaacg
     7801 cgagatggtg gtgattggcc tgagcgggcg tggtggcggc aaaatgctgc aggcgctgcg
     7861 cgataccgat gtgcacgttt gcgtacccca cgaccgtact gcccgcattc aggaggtgca
     7921 cttgctgacg ctgcattgcc tgtgtgacgc agtggacacc ctgctacttg gcgatttgga
     7981 gaacccacca tgaaacacac aactcaacac ctgttcaccc tgctggcttt gacggccagc
     8041 ctgggcggta tcctgaccgc ctgcgtgccg gtgatcatcg gtggggccgt cgtgggcacc
     8101 gccatggtgg catccgaccg acgcacctcg ggtgcccagc tcgaagatga aggcattgag
     8161 ctgcgcgcgg ccagccgcct tcaggaggcg ctgggtgaca ggctgcacgt caacgtaacc
     8221 agctacaacc ggcgtgtctt gctgaccggc gaggtgccag cggctcagga caagcagtcg
     8281 gcggaacaaa ttgtgtcaaa ggttgacaac gtcgagtctg tcgtcaacga actgatggtg
     8341 gccggcaaca gcaatatctc tgaccgctcg tcagatgcac tgattaccgg caaggtcaag
     8401 gccgggctgg tcgatgccaa ggacctgttt gccaacgcgt tcaaggtcgt gaccgaacgt
     8461 ggtgccgtct acatgatggg gcgggtgacc cagcgtgagg cagaccgtgc caccgaagtt
     8521 gcccgcagca ccagcggtgt gaaaaaggtg gtgcgtgttt ttgagttcat cagtgaagat
     8581 gaactgcgtg gcatgatgcc caagccagca ccgggggcag ccaaaaacta gcgcccgtcc
     8641 tggtgcaact tatttcagcc gcttgatcag gctggaggtg tcccagcgat tgccgcctag
     8701 ctgctgcacg tccgcgtaaa actggtccac cagggcggtt accggcaagc gcg
//
LOCUS       DAOSLL010000128         8708 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7580_length_8708_cov_5.164452, whole genome shotgun sequence.
ACCESSION   DAOSLL010000128 DAOSLL010000000
VERSION     DAOSLL010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8708)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7580_length_8708_cov_5.164452"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="PLB25_07480"
     CDS             <1..628
                     /locus_tag="PLB25_07480"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOE41456.1"
                     /translation="DLKAAGVDIKFNGIGTGRGTLALIDGKVSMATVGDTLEDSVGAA
                     KKAAKAENRDISAPANLVYTKIGEDELVVIVHKSNPVTALSKAQLKDIGTGKITNWKD
                     VGGADLPIKVVATEPSLAPGQFFKKAMMDGADYVKGTTEVRSPKEVITWVSRTPGGFG
                     AAADVHMKAAPGDAKIVKSPSMVRPMGLITVGQPTGNGKKVADLLKKS"
     gene            1074..2825
                     /locus_tag="PLB25_07485"
     CDS             1074..2825
                     /locus_tag="PLB25_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE41457.1"
                     /translation="MFERITINARLMMLAGGLLLALLVTGLMGVRGIASVTRDYERDA
                     TAMVGLNEVMDSLHLRRGSILQAMGESAASEAEAPFKLADQASTDLNASWKTYLAGAL
                     ESEKLLAEKFSQTWKAYQDSSKQTIDLARAGDYEKAADNMKTDAATKFTATRLALMAL
                     LAQKKVEIAATNRVNSQIINTVLAVLVLALLAGSFGYWAIRRAILQPLHEAIRVTEQI
                     AGGDMSQAIAVRSQDEIGRLMRALSDMQASLSGVVARVRHGSESVATASVEIAQGNSD
                     LSARTESQASALEQTAASMEELSSNVKQNADNARQANQLAMSASTVAVQGGEVVAQVV
                     ETMKGINEASRRIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSL
                     AGRSAEAAKEIKALISASVGRVEQGTALVDQAGTTMQEVVTSIKRVTDIMGAISTASN
                     EQSQGVGQVGEAVTQMDQVTQQNAALVEEMAAAASSLKSQAQELVQTMAVFKLGAGDS
                     THMAIPTAAVRSHPPKVSNFKGADRRAGGVPKGAAAREHTAAPSRPAPKPKAPTPLAA
                     PAATSSTATSDSDWETF"
     gene            3117..3341
                     /locus_tag="PLB25_07490"
     CDS             3117..3341
                     /locus_tag="PLB25_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41458.1"
                     /translation="MEISFKIGGPEVDAQHQHLFELINKLVAVDGMHDLRPPPAHSGH
                     LCHGYGLAELGIPNQCTVAVNDCKRVGNTP"
     gene            complement(3388..3474)
                     /locus_tag="PLB25_07495"
                     /pseudo
     CDS             complement(3388..3474)
                     /locus_tag="PLB25_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011600735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(3487..3795)
                     /locus_tag="PLB25_07500"
     CDS             complement(3487..3795)
                     /locus_tag="PLB25_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013690204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HOE41459.1"
                     /translation="MTRPVNRMRPVHPGEVLREDYLVPIGLSVNALAVALGVPATRIH
                     EIVKERRAVTADTAARLAKHFGGDATSWLVMQANYDLKTLPTLGDIERAVPVRQQLAA
                     "
     gene            complement(3806..4084)
                     /locus_tag="PLB25_07505"
     CDS             complement(3806..4084)
                     /locus_tag="PLB25_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOE41460.1"
                     /translation="MIQSFKCQDTQALYEGKCPRRFKALAKVAERKLAQLDAAQSLAF
                     MKAPPGNHWEALKDDRKGQHSIRINDQWRICFVWTEVGPTDIETLDYH"
     gene            complement(4548..5015)
                     /locus_tag="PLB25_07510"
     CDS             complement(4548..5015)
                     /locus_tag="PLB25_07510"
                     /inference="COORDINATES: protein motif:HMM:NF016232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE41461.1"
                     /translation="MSLAGQLLDQKSLRAVTSKTADWNEISKYCIAFANATGARLLLG
                     IEDGQDDPPASQHIPADLPDTLRRKLTERTVNVAVIPDVVTAPNGGQYIELRVPRALA
                     VASTTDGRHFLRVADQSKPITGDDVMQLASERSALPWETQTTLHMPRVEVDAA"
     gene            5321..5857
                     /locus_tag="PLB25_07515"
     CDS             5321..5857
                     /locus_tag="PLB25_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcription factor"
                     /protein_id="HOE41462.1"
                     /translation="MRLTTKGRFAVTAMIDLGLRQAAGPVTLAGISQRQEISLSYLEQ
                     LFGKLRRHSLVESTRGPGGGYTLARAPAEITVAEIISSVDEPMDATHCGGKENCLGEG
                     ARCMTHELWASLNVKMVEFLESISLQKLVDEQLAKGFQMEEKPLLKRAISSMPAAKPI
                     RVNAPNSVFALGNIYSKM"
     gene            5894..7114
                     /locus_tag="PLB25_07520"
     CDS             5894..7114
                     /locus_tag="PLB25_07520"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HOE41463.1"
                     /translation="MDSTPHFPVYMDYSATNPCDERVVDAMIPWLRQHFGNPASRSHA
                     WGWEAEAAVEKAREQVAALIGADPREIVWTSGATESNNLAIKGAAHFYQSKGKHIITV
                     KTEHKAVLDTCRELERQGFAVTYLDVQPDGLVDLEVFKAAIRPDTILASVMFVNNEIG
                     VIQDIAALGAICRSKGVVFHVDAAQATGRVEFDLGQLPVDLMSLTAHKTYGPKGIGAL
                     FVRRKPRIRIEAQMHGGGHERGMRSGTLPTHQIVGMGEAYAIAKAEMAVENRRIQALH
                     DRMIAGLSDIEQVFINGNLAQRVPHNLNMSFNYVEGESLIMGIKGLAVSSGSACTSAS
                     LEPSYVLRALGRSDELAHSSLRMTIGRWTTEAEIDYAIDTIRVNVARLRGLSPLWDMY
                     QEGIDISTIQWAAH"
     gene            7234..7632
                     /gene="iscU"
                     /locus_tag="PLB25_07525"
     CDS             7234..7632
                     /gene="iscU"
                     /locus_tag="PLB25_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HOE41464.1"
                     /translation="MAYSEKVVDHYEHPRNVGSFDKGDESVGTGMVGAPACGDVMKLQ
                     IRVNAETGVIEDARFKTYGCGSAIASSSLVTEWVKGKTLDQASALKNSQIAEELALPP
                     VKIHCSILAEDAIKAAVNDYRQKHTEAQTV"
     gene            7702..8025
                     /gene="iscA"
                     /locus_tag="PLB25_07530"
     CDS             7702..8025
                     /gene="iscA"
                     /locus_tag="PLB25_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HOE41465.1"
                     /translation="MAVTLTEAAARHVNRYLSKRGLGVGVRLGVKTTGCSGLAYKLEY
                     VDEVGPEDMVFEGHGVKVLIDPKSFAYLDGTELDFVREGLNEGFKFNNPRERDRCGCG
                     ESFRV"
     gene            8022..8540
                     /gene="hscB"
                     /locus_tag="PLB25_07535"
     CDS             8022..8540
                     /gene="hscB"
                     /locus_tag="PLB25_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HOE41466.1"
                     /translation="MNLQSDDFELFGLQRRFAQDRTQLDARWKDLQRQAHPDKFAAQG
                     AAAQRIAMQWSVRINEAYQRLKTPIKRAAYLCELQGAPVNAENNTAMPAVFLLQQMQW
                     RDDLEAAHSPESLEQIRLQVKQVVRDMLQKIEQLMDHQGAYSEAAAEVRALMFIERFE
                     QDVDSRLDQIET"
BASE COUNT         1920 a         2434 c         2623 g         1731 t
ORIGIN      
        1 cgacctcaag gcagcggggg tcgacatcaa gttcaacggc atcggcaccg gccgcggcac
       61 acttgccctg atagatggca aagtcagcat ggcgaccgtg ggcgacacgc tggaggactc
      121 cgtaggtgcc gccaaaaaag ccgccaaggc agaaaaccgc gatatcagcg cgccggccaa
      181 cctggtgtac accaaaattg gcgaagatga actggtggtg attgttcaca agagcaaccc
      241 ggtgacggcc ttaagcaaag cgcagctcaa ggacatcggc accggcaaga tcaccaactg
      301 gaaagatgtc gggggggctg atctgccgat caaggtcgtg gccaccgagc cctcgctggc
      361 accgggccag tttttcaaga aggccatgat ggatggtgca gactacgtga aaggcaccac
      421 cgaggtacgc tcacccaagg aagtcattac ctgggtgtca cgcacgcccg gtggttttgg
      481 tgctgcagcc gatgtccaca tgaaggccgc ccccggcgat gccaagattg tcaagagccc
      541 ttccatggtg cgccccatgg gcctgattac cgtggggcag cccacgggca acggcaaaaa
      601 agtggccgac ctgctcaaga agagctaagg gcttgttctc aaataagatg cacatttggc
      661 tcgccagatt tgggcgcact gcgttgttgc aaatcgcttg gtgcgtgagt tgcatgtcat
      721 gctttgttcc tagcattgca tcccagctcc gacgacccaa tgcacggcat atctataaac
      781 aagcccttag ggaagcagac aatacaaaag taccaacatc gtcagggcga gccttccgaa
      841 tcaggcccga cactgcacac gcaccttgaa taaggatgcg tcactgtgta cgaagcgagg
      901 cagtcgatgc ctgtgatggg gaaaaaaata agtctccgcg tttcgctcgc aatgacagaa
      961 aacgataccg tggcaatttc caaccctctg aacttttttt gcctcaaccg ggtatggcgc
     1021 tgttggcggc gcgtcatacc ctgaatgcgc gcccgctatg aaagaggtgt tccatgtttg
     1081 aacgaatcac aatcaacgcc aggctgatga tgctggcggg cggcttgctg ctagccctgc
     1141 tggtcacagg cctgatgggt gtgcgcggta ttgccagcgt gacgcgcgac tatgagcgcg
     1201 atgccacagc catggtgggc ctcaacgaag tcatggacag cctgcacttg cgccgtggca
     1261 gcatcctgca ggccatgggc gagagcgccg ccagcgaggc cgaggccccc ttcaaactgg
     1321 cagaccaggc cagcacagat ctgaacgcgt cctggaagac ctacctggct ggcgcgctgg
     1381 aaagtgaaaa actactggca gaaaaattca gccagacctg gaaagcttac caggacagca
     1441 gcaagcaaac cattgatctt gccagggcag gtgactacga aaaagccgcc gacaacatga
     1501 agacggatgc cgcgacaaaa tttaccgcca cgcgcctggc gcttatggcc ttgctggcgc
     1561 agaaaaaggt ggaaattgcg gccaccaacc gggtcaatag ccagatcatc aacacggtgc
     1621 tggctgtgct ggtgctcgcc ttgctggcag gtagcttcgg ttattgggcc atacggcgcg
     1681 ccattttgca gccgctgcat gaggccatac gggtgaccga acagattgcc ggcggtgaca
     1741 tgtcgcaggc cattgcggtg cgcagccagg acgaaattgg ccgtttgatg cgagccctta
     1801 gcgacatgca agccagcctg tcaggcgtgg tggcccgcgt gcgtcacggc agcgaatccg
     1861 tggccaccgc cagcgtcgag attgcccagg gcaatagcga cttgagcgcc cgcacagagt
     1921 cgcaggccag cgcgctggag caaaccgctg ccagcatgga agagttgtct tccaacgtca
     1981 aacaaaacgc cgacaacgcc cgccaggcca accagttggc catgagtgcc agcaccgtgg
     2041 cggtgcaagg tggcgaggtg gttgcccaag tggttgaaac catgaaaggc atcaatgagg
     2101 ccagccgcag gattgccgac atcatcagtg tgattgacgg cattgctttt cagaccaaca
     2161 tactggcgct gaacgcggca gtggaagccg ctcgcgccgg ggagcagggt cgtggctttg
     2221 ccgtggtggc cagtgaggtg cggtctctgg ccgggcgcag cgccgaggca gccaaggaaa
     2281 tcaaggccct tatcagcgcc agcgtggggc gggtcgagca aggcactgcg ctggtggacc
     2341 aggccggcac caccatgcag gaggtggtga ccagcatcaa acgtgttacc gacatcatgg
     2401 gtgcgatcag caccgccagc aacgaacaaa gccagggtgt cggacaggtg ggcgaggccg
     2461 tcactcagat ggaccaagtg acccagcaaa acgctgccct ggtggaagaa atggcggcag
     2521 cggccagcag cctgaagagc caggcacaag agctggtgca aaccatggca gtgttcaaac
     2581 tgggggctgg cgacagcacc cacatggcga taccgactgc agcagtgcgc tcgcacccgc
     2641 ccaaggtgag caacttcaag ggcgctgacc ggcgcgccgg tggtgtgccc aagggtgccg
     2701 ctgcacgcga acacacggct gcaccgagcc gaccagcacc caaacccaag gcaccaacgc
     2761 cattggctgc gcctgctgcc acatcttcaa cggcgacaag cgactcggat tgggaaactt
     2821 tctgatctga aactgctgtt taatacatag tttctattca atagccaatc agggcttgtg
     2881 atcagaaaga cgcaaacttt ctcgccactg cgcctcagac cggcaagcgc cgcactcaag
     2941 gcgcccggtt gtcactttct cgcaggaaat acgcaacggc tagggtttgc ccatgccagc
     3001 gctgcagagg gccttggcgc tttcaagcca gccagcgtgt gccgggtaag cctgggttga
     3061 gtatttgggt tatggtcgat tccaatttca gttgaattcg gctatcggag cgttcaatgg
     3121 aaatcagttt caagattggt gggcccgagg ttgatgccca gcatcagcac ctgtttgaac
     3181 tgatcaacaa actggtggcg gtggatggca tgcatgattt gcggccgcca ccggcgcact
     3241 ctggtcatct ttgccacggg tatggtctgg cagagttggg cattcccaac cagtgcactg
     3301 tggcggtgaa cgactgtaag cgggtgggca acacgccttg acgtgtagga gccttgatgg
     3361 caaggcggtg cgcagttgcg tttttgtggg ctgtaagcgg tgaggcagca attcttcagt
     3421 ctgactgttc ttctgtgtcg gcagacgcgt caacacgtcc ttgatgtagg cataggcttg
     3481 gctgcgctat gcggcgagtt gttggcgtac tggaaccgcg cgttcaatgt ccccgagggt
     3541 tggcagagtt ttcaagtcgt agttggcttg cataaccagc catgacgttg cgtcaccgcc
     3601 aaagtgcttg gccaagcgtg ccgcagtgtc agccgtaact gcacgtcgct ctttgacgat
     3661 ctcgtgaatg cgagtcgcgg gtacacccaa agcgacggcg agggcgttga cgctcagccc
     3721 gatcgggaca agatagtctt cgcgcagaac ctcgccgggg tgcacgggac gcatacgatt
     3781 gacgggacgt gtcatattta ttccttcagt gatagtccaa ggtctcaatg tcagtgggcc
     3841 caacttcagt ccatacgaag cagatgcgcc actggtcatt gatgcggatg ctgtgctggc
     3901 ccttccgatc atctttcagc gcctcccagt gattcccagg cggcgccttc atgaatgcca
     3961 gtgactgcgc ggcatcaagc tgcgccaact tgcgctcggc aaccttggcc agagctttga
     4021 accggcgagg acactttccc tcgtagagcg cctgggtatc ctggcacttg aacgactgaa
     4081 tcattcgcga agtgtattac gtttcgcgta agatggtaag acggatttcg ctggggaatt
     4141 tcagaaggga tgttctcatg aaaccgaacg ttcaaggtaa ccggcgctgc gcgactttgt
     4201 cacgcagcgt ccgtgttgac cgcagtgctg gacgaagccg ctacacggag gtttcggtaa
     4261 ctagtagtcc gtttcatcaa gttggataca aattgaaatt gattgatttt tccgtctggc
     4321 taggcgcgag gaggcgacat agccgtagct tgtgccagga cgagcaacaa cgccaggcgg
     4381 gaaaagacac gatttcatgc attcgttttg gtggaacgaa ttactaggtt ggtcaaagcg
     4441 tttcccggcg cgagctgata gtgatcaagc agctcgtcgt cggtcttttc cttgaccgag
     4501 gccttgacgc ggtcggaggc gcgtaacgct tgcagccact tgtcacgcta ggcggcatcc
     4561 acctcgacgc gcggcatatg cagggtggtt tgtgtttccc acggcagcgc ggagcgctcg
     4621 ctggccagct gcatcacgtc gtcgcccgtg atgggcttgc tctgatcggc cacgcgcaga
     4681 aagtggcggc cgtcggtcgt cgaggccaca gccaaggcgc gcggaacacg caattcaatg
     4741 tactggccgc cattgggggc agtgacgaca tcgggtatca cggcaacgtt gacggtgcgc
     4801 tcagtcagtt tgcgtcgcag ggtgtcaggc aggtcggcgg ggatatgttg actggcgggc
     4861 gggtcatcct gcccatcctc gatgcccagc agcagccgag cgccggttgc attggcgaag
     4921 gcgatgcaat acttggagat ttcgttccag tctgcggtct tgctggtcac tgcccgcaaa
     4981 gacttctggt ccaggagttg tcctgccagg ctcaaaggca tcctccaccc aagtcatgca
     5041 tcctgatatc cctttgccag ttgtcacact ggcatgggtc taacggacgc ctgcgcagtc
     5101 aaaggacggg ctatttcctg cggtggcgat tgggtcaccc agcttgatgc gacaacatcg
     5161 cggtgctcac cggcagcaga aatcactcaa tacccttaaa aagttactga tcagtctagt
     5221 tacccgacta gaacgctcgg attttggtta tacttgactt cgctagtcga gaaagcactt
     5281 gaccaagcct gtttacccaa ccgaatgcca ggagttcctc atgcgactta ccaccaaagg
     5341 ccgttttgcc gtcacagcga tgattgatct ggggctgcgc caagctgccg gcccggttac
     5401 cctggccggg atcagccagc gccaggaaat ctcgctgtcc taccttgagc agctttttgg
     5461 caagttgcgc cgccattcgc tggtggaatc cacccgtggc cctggcggtg gttacaccct
     5521 ggcgcgggcg cccgctgaaa ttaccgtggc cgaaatcatc agctcggttg atgaaccgat
     5581 ggatgcgact cactgcggtg gcaaggaaaa ctgcctcggt gagggtgcgc gctgcatgac
     5641 gcacgagttg tgggcctcgc tgaacgtcaa aatggtcgag tttctggaat cgatcagtct
     5701 gcaaaaactg gtcgatgagc agttggccaa gggttttcag atggaggaaa agccactgct
     5761 caagcgcgcc atctccagca tgccagcggc caagccgatt cgcgtcaacg cacccaattc
     5821 ggtgttcgcc ttgggcaaca tctattccaa gatgtgaccc gattgccatc tcaattcagc
     5881 caaagacccc accatggaca gcacccctca ttttcccgtt tacatggact acagcgccac
     5941 caacccttgc gacgagcggg tggttgatgc catgatcccc tggttgcgcc agcattttgg
     6001 caacccggcc tcacgtagcc acgcctgggg ctgggaagcc gaggctgccg tcgaaaaggc
     6061 gcgcgagcag gtggcggcgc tgattggtgc cgacccgcgc gaaatcgtct ggacctcggg
     6121 tgccaccgag tccaacaacc tggcgatcaa gggtgcggcg cacttctacc aaagcaaggg
     6181 caaacacatc atcaccgtca agaccgagca caaggcggtg cttgacacct gccgcgagct
     6241 ggagcgccag ggctttgcgg tgacttacct tgatgttcag ccagacggtc tggttgatct
     6301 ggaagtcttc aaggcggcca ttcgccccga caccattttg gccagcgtga tgtttgtcaa
     6361 caacgaaatt ggcgtgatcc aggacatcgc ggcgctcggt gccatttgcc gcagcaaggg
     6421 cgtggtgttc catgtcgatg cggcgcaggc caccgggcgg gttgaatttg atctgggcca
     6481 gttgccggtt gatttgatga gcctgaccgc ccacaaaacc tacggtccga aaggcattgg
     6541 cgcgctgttt gtgcgacgca agccgcgcat tcgcatcgag gcgcaaatgc acggcggcgg
     6601 tcacgaacgc ggcatgcgca gcggcacgct gccaacccac cagatcgttg gcatgggcga
     6661 ggcgtacgcc atagccaagg cagaaatggc agtcgagaac cggcgcatcc aggcgctgca
     6721 cgaccgcatg attgccgggt tgagcgatat cgagcaggtg ttcatcaatg gcaacctcgc
     6781 acagcgggta ccgcacaacc tgaacatgag cttcaactat gttgagggtg agtcgctgat
     6841 catgggcatc aagggcctgg cagtgtcgag tggttcggcc tgcacgtcgg ccagtctgga
     6901 gcccagctat gtgctgcgcg cgctgggccg cagtgacgaa ctggcgcaca gcagcttgcg
     6961 catgacgatt ggccgctgga ccaccgaagc cgagattgat tacgcgattg acaccatccg
     7021 ggtcaatgtg gcccggctgc ggggtcttag tccgctgtgg gacatgtacc aggaaggcat
     7081 cgatatatcg accattcaat gggcggctca ctgatagatg aagcctgggg gtcgtttgtc
     7141 ggtccgatct ttttgacctg atcgaccgag attgaccccg cagaagtcta ttgagcctga
     7201 ataaaaagtc tcttcatccc gaaaggaaac gctatggcat attctgaaaa agtggttgac
     7261 cactacgaac atccgcgcaa tgtgggttcg tttgacaagg gcgacgagtc ggttggcacc
     7321 ggcatggtcg gcgcaccggc ttgtggcgac gtgatgaaac tgcagatcag ggtcaatgcc
     7381 gaaaccgggg tgattgaaga tgcccgcttc aaaacctatg gttgcggctc ggcgattgcc
     7441 tcaagctcgc tggtcaccga atgggtcaag ggcaagacgc tggaccaggc ctctgcgcta
     7501 aaaaatagcc agatcgccga agaattggcc ttgccgcctg tcaaaatcca ttgttcgatc
     7561 ctggctgaag atgccatcaa ggcagcggta aatgactacc gccagaagca caccgaagca
     7621 cagaccgtct gaaacaccaa cgacctgacc ggcatgcaat ggcaggctag gtcggcaacc
     7681 ccctaacaac atacaagaat catggctgtt acgctgactg aagctgccgc ccggcatgtc
     7741 aatcgctacc tgagcaaacg cggattgggt gtaggcgtgc gtctgggtgt caaaaccacc
     7801 ggttgttcgg ggctggccta caagctggag tacgtcgatg aagtcgggcc tgaggacatg
     7861 gtgtttgaag gccatggtgt caaggtgctg attgacccca agagttttgc ctatctcgat
     7921 ggcaccgagc ttgactttgt gcgcgagggc ctcaatgaag gcttcaagtt caataacccc
     7981 agagaacggg accgctgcgg ttgtggtgag tcgtttcggg tgtgaatctc cagtctgacg
     8041 atttcgagct gtttggcttg cagcggcgct ttgcgcaaga ccgcacgcag cttgatgcgc
     8101 gctggaaaga cctgcagcgc caagcgcacc cggacaagtt tgccgcccag ggtgctgccg
     8161 cccaacgcat tgcgatgcaa tggtcggtgc gcatcaacga ggcttaccag cggctgaaaa
     8221 caccaatcaa acgcgctgct tacctgtgtg aactgcaggg tgcaccggtc aacgcggaaa
     8281 acaacaccgc catgccggct gtgtttttgc tgcagcaaat gcaatggcgt gacgatctgg
     8341 aggccgcgca cagccccgaa tctttagagc aaatccggct gcaggtcaag caggttgtgc
     8401 gtgacatgct acaaaaaata gagcaactga tggaccatca gggtgcctac agcgaggctg
     8461 cagccgaagt ccgggcgctg atgttcatcg agcgttttga gcaagatgtt gatagccgcc
     8521 tggaccagat tgaaacctga gacgaccagc ttgaggcagc agcgccgcct tgccagtcag
     8581 ggcaagccct caagcccgac gcattcaccc taacttttga cctagattcc tcagttgacc
     8641 gcttgttttc aatggtaagt cctcatgaat attgactttt tcagcttcga tggggttcac
     8701 ccattggg
//
LOCUS       DAOSLL010000129         8684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7609_length_8684_cov_4.122842, whole genome shotgun sequence.
ACCESSION   DAOSLL010000129 DAOSLL010000000
VERSION     DAOSLL010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8684
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7609_length_8684_cov_4.122842"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="PLB25_07540"
     CDS             <1..598
                     /locus_tag="PLB25_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014898193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE41467.1"
                     /translation="ESYGLTEASPGVTSNPLNIEDWTGDIGLPFPSTQVRVVDDNDVE
                     VASGQVGEICVRGPQVMAGYWNQPQETAHVFTADGWLRTGDMGLMNEAGSLRITDRKK
                     DIIIVSGFKVFPNQIEDVVASHLGVLEVGAIGVPNEHSGESVKIVVVKRDPSLTEQDL
                     LDHCRQHLTGYKMPSIVEFRVNPLPKSNIGKILRRQLR"
     gene            1002..1778
                     /locus_tag="PLB25_07545"
     CDS             1002..1778
                     /locus_tag="PLB25_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004266268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HOE41468.1"
                     /translation="MNTEVPLARRDQILERLEQGKPAVASSLATEFNVSEDAIRRDLR
                     ALAAEGRCRRVYGGALPILASSKPMAARVGEDWPRKEALARAAAQTIQADEFIFLDVG
                     STNLALVDHLPEDKGLTVATNSIDIAKAVLLRGDLTLIMVGGAVDTQVGGCIDASAIK
                     AVADMNIDRCFIGACALSAKFGLGAIHHGDALFKRALLSASQIRIAMVTNEKLTAKGP
                     HRISAAKNLDQVILEEDARSEIVVELQGADVPVLLAKTST"
     gene            1826..2998
                     /locus_tag="PLB25_07550"
     CDS             1826..2998
                     /locus_tag="PLB25_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007580831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE41469.1"
                     /translation="MTHSNKSSSRVATRLAFWVAGFGLSAWAPLVPYAKQRLQIDDRS
                     LGLLLLCLGIGSVAAMVRTGPLCARYGCKPVILTGSLCMALLLPALALADSVWLMAMA
                     LVIFGGALGSLDVAMNVHAVEVERNAGRPMMSGFHALFSVGGFSGAALVTVMLSHSFA
                     PSTATFVCSAMMVVAMALTTPRLLATATLHDGQSGTFMALPRGPVVILSVLAAIAFLV
                     EGALLDWSALLLTNMGLSTPQLAGVGYAMFSVAMTAGRFGGDAITARFGDRAAVFWGG
                     LVVLCGFICLLTLHSYEMAMVGFVLIGLGASNVVPVLFRQAGAQSAMPTAMAISAVTT
                     IGYAGQLMGPAVMGFISKLAGLQAAFWMLAALMCLVPVFARTASSPKGVVTNPSAI"
     gene            3832..4989
                     /locus_tag="PLB25_07555"
     CDS             3832..4989
                     /locus_tag="PLB25_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal
                     protein"
                     /protein_id="HOE41470.1"
                     /translation="MKVRTFLKVLGGAIILPATLGLPGCSAEIPSEATSGWRGPDPEA
                     DIRRWVLSYALLAPHSHNLQSWVADLRQPDEIVLRCDLSRLLPETDPYFRQIMMSHGT
                     FLELLDLAARERGYKSDITLFPEGSFDALQLDARPVARIRLLPDASIQKDPLFAQILK
                     RRTNRSIYDAQRAVPTQAWQAMSKALGSAAVLLDYVDLAKPEAMAQHRKIASDAWRIE
                     MTTPRTILESYKVLRVGADEILKHRDGLSIMDPKLVWLDRLGLFDRSRAPAPDAYATT
                     SQIKEFDAKIDSTPAFLWVKTARNDRVAQIAAGRAYARVQLAATAHGVAMQPLQQALQ
                     EYTEQQANHQAIHQLCGATPSGQTVQMWARVGFAPAVPAAPRRPLAEILRT"
     gene            5123..5743
                     /locus_tag="PLB25_07560"
     CDS             5123..5743
                     /locus_tag="PLB25_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE41471.1"
                     /translation="MTKLPTRNRDLTSAKIIEAVGRVLARDGIDKIGINAIAREAGVD
                     KVLIYRYFEGLPKLLTAWAASGEFWPSVQELVDISPPNLMELPLAERYVFFFDHFIDS
                     LRARPLTIEILAGEISHRNALTDIMEDQREKWGEEVGRTLAALSEYQAHPALLPLTNV
                     LIAGVQNLLIRARSIHSFGGLDIQSDEGWNLVKKSIGVMANKLFKP"
     gene            5740..6888
                     /locus_tag="PLB25_07565"
     CDS             5740..6888
                     /locus_tag="PLB25_07565"
                     /inference="COORDINATES: protein motif:HMM:NF020243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HOE41472.1"
                     /translation="MNIFLRMRPQKLLILGGYGNAGLAIARLLAKVGDLQIVLAGRRI
                     QCAANAARQMDAEFGTDRFSGLEVNAANRDSLLAAFRQVDLVIVAASTIEHTKVVAEA
                     ALEAGIDYLDVQISVKAKHQALESLRERIRTAELCFITDGGFRPGIPAAMVRYAARQM
                     PGLESAAVASAFQVNWRDRAFSSSSAVEFTDELKSFSPRVLKDGVWQSASLRAYPQVD
                     FGPPFGIKYCAPMFMEEFRELPDLIPTLRNTGFYSSGLGGFVDYILIPLAFGLLALSP
                     EGSRHWIGRLMEWGLKHTTHPPYGAVLQMDARGPKCALRMTVSHADAYLVTAAPTVAC
                     LLQYFDGSLRKPGLWKQATLVEPVQFFDDLTRLGLDVKVQAMDAPQPM"
     gene            6952..7344
                     /locus_tag="PLB25_07570"
     CDS             6952..7344
                     /locus_tag="PLB25_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41473.1"
                     /translation="MFDISKRVFSLCLALASAEMLHGITRIRYLVPLVGKSNAQKISI
                     VTGTLLAFGVCYVFIPAMGLTTRFSAIALGVFLAAFMASFDIAVGRLLMRRKWALIAE
                     DFDPRKGNYLVFGLAALVAIPTFVLRIA"
     gene            7927..8118
                     /locus_tag="PLB25_07575"
     CDS             7927..8118
                     /locus_tag="PLB25_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41474.1"
                     /translation="MADDDARSLRWREAFIASYLERDIPALGPKVPAATLRRLWTMLA
                     HNQGAMLNQSQLAGALAAI"
     gene            8103..8273
                     /locus_tag="PLB25_07580"
                     /pseudo
     CDS             8103..8273
                     /locus_tag="PLB25_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867639.1"
                     /note="catalyzes the radical-mediated insertion of two
                     sulfur atoms into an acyl carrier protein (ACP) bound to
                     an octanoyl group to produce a lipoyl group; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
     gene            8331..8483
                     /locus_tag="PLB25_07585"
     CDS             8331..8483
                     /locus_tag="PLB25_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41475.1"
                     /translation="MQTHWQSSSLWLDLASLEQQCDSISCARALELYRMGLGKSLRTL
                     AHLQIE"
     gene            8537..8684
                     /locus_tag="PLB25_07590"
                     /pseudo
     CDS             8537..8684
                     /locus_tag="PLB25_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466192.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
BASE COUNT         1825 a         2422 c         2424 g         2013 t
ORIGIN      
        1 cgagagctac gggttgaccg aggcatcacc tggggtcacc tccaacccac tgaacatcga
       61 agactggacc ggcgatattg gcttgccgtt tccctccact caggtccgcg tggtggacga
      121 caacgatgtc gaagtcgcaa gcggccaggt tggcgagatt tgcgtgcgtg gcccgcaagt
      181 gatggccggc tattggaatc aaccgcaaga gacggcacac gtattcaccg ctgacggttg
      241 gttgcgcacc ggcgacatgg ggttgatgaa cgaagcgggc agcttgcgca tcactgaccg
      301 caagaaggac atcatcatcg tctcgggttt caaggtgttc ccaaatcaga tcgaagacgt
      361 cgttgccagc catctcggcg tacttgaggt gggtgctatc ggggtgccca atgaacactc
      421 tggtgaaagc gtgaagatag tcgtcgtcaa aagggatccc tcgctgaccg agcaggacct
      481 gctcgaccac tgtcgccaac atctgaccgg ttacaaaatg ccaagcattg tcgagttcag
      541 agtcaaccca ctgcccaaat ccaacatcgg aaaaatcttg cgccgccagt tgcgctgagt
      601 cggtgattcg tgattcgcgg atgtcgactt taaggataat ttgcgcggca gccgtgtctg
      661 caatgggcac cttggagccg cccaccaacg tcggctggtc ggcacctcaa atcatgaatt
      721 tgaacttccc ataaccgggc attggtcgcc gtccgcttac ccaaagcggt aattgccatc
      781 accaaaatca acgcccggta gccgacattc acgcatctgc agcatcttta tgccaatgtc
      841 cgctaaccag attgccgatc ttcagagtgg tagtcgcgag ttggccacaa ccggtcagat
      901 gcattcatca cataaagcag tcatttgatc aacggccttg ctaactgtga ttcacgttta
      961 tacacgttgc attcttgttt gtacacgttt agaatcatgc catgaacacc gaagttcccc
     1021 ttgcacgcag ggaccaaatc cttgagcggc ttgaacaagg caagcctgct gtggccagtt
     1081 cactggcgac agagttcaac gtttcggaag atgccatacg gcgagacctt cgcgctctgg
     1141 ctgccgaagg gcgatgccgt cgtgtctacg gaggtgcact ccccatcctt gccagcagca
     1201 agcccatggc cgctcgtgtt ggggaagact ggcctcgcaa ggaggcattg gcgcgtgccg
     1261 ccgcacaaac tatccaggcg gatgagttca tatttctgga cgtcggcagt accaatctgg
     1321 cattggttga tcacctgcca gaggacaaag gcctcacggt ggccaccaat tcgatcgaca
     1381 ttgccaaggc ggttttgttg cgcggcgatt tgacactgat catggtcggt ggcgcagtgg
     1441 acacgcaggt tggtggatgc atcgatgcat ccgccataaa ggctgtggct gacatgaaca
     1501 ttgaccgttg cttcatcggt gcctgtgcgc tttcagccaa atttggactg ggtgccatcc
     1561 accatggcga tgcactcttc aagcgggctt tgctttcagc aagccagata cgtattgcca
     1621 tggtcacgaa cgaaaagctc acggcaaagg gtccccatcg gatttcagcg gcaaagaatc
     1681 tggatcaagt catcctggag gaagacgctc gatccgaaat agttgtggaa cttcaaggtg
     1741 cagatgttcc agtcctattg gcaaagacat ccacttgagt acgccttaca actgatccag
     1801 cgcaacattt cttttgcaac ttcacatgac ccattcaaac aaatcctcct cgcgagtggc
     1861 aacccggctt gccttttggg tcgctggctt tggactctct gcctgggcac cgctggttcc
     1921 atacgccaag caacgcctgc aaatcgacga ccgatcactt ggcctgctgc tcttgtgcct
     1981 gggcataggt tcggttgcgg caatggttcg cacggggcca ctgtgcgcac gatatggttg
     2041 caaacctgtc atcctcactg gaagcctgtg catggctttg ctactgcctg ctttggcact
     2101 cgcggattcg gtgtggctga tggcaatggc gctggtgatt ttcggtggcg cattgggctc
     2161 attggatgtt gcaatgaacg tccatgctgt tgaggtagag cgcaatgcgg gtcgtccgat
     2221 gatgtccggc tttcatgcgc tattcagcgt tggtgggttc tcaggtgcag ctttggtgac
     2281 tgtgatgttg tcccattcct ttgctccgtc aacggctact tttgtttgct cggcaatgat
     2341 ggtggtggcg atggcactga ccaccccgcg tctgcttgcc acggccactt tgcatgatgg
     2401 gcagtcaggg acattcatgg ctctaccaag agggccagtt gtgattttgt ctgtgttggc
     2461 ggcaattgca tttctggtgg aaggcgcact gttggattgg agtgcactcc ttctcaccaa
     2521 catgggtctg agcacaccgc aactcgctgg cgtcggatac gctatgtttt cagtggcaat
     2581 gacagcgggg cgtttcggtg gtgatgccat cacagcccgc tttggtgaca gggcggcggt
     2641 gttctggggt ggtttagtcg tcttgtgcgg tttcatatgc ctgcttaccc tgcacagcta
     2701 cgagatggcc atggttggct ttgtgctgat tggcctgggt gcttcaaacg tcgttccggt
     2761 gttgtttcgc caagccggag cacaaagcgc gatgcccacc gccatggcga tctctgccgt
     2821 tacaaccatt ggatacgctg gccagctcat ggggccagcg gtcatgggct ttatctccaa
     2881 gctggcaggt ttgcaagctg cgttctggat gctggctgca ttgatgtgcc tggtgccagt
     2941 ctttgcacgt actgcaagca gcccaaaggg ggttgtgaca aacccaagcg caatatgaaa
     3001 ctcgcacacg ttgcgctttg gacacctgat cttgactgtg ctgtcgcgtt ctggatccgc
     3061 tacttcaatg tgacatcaga tacgacctac ttgagcgagc ggcatgctgg attcgcatcc
     3121 cgctttgtag aacctccagg cagcgggacc aggattgaag tcatggcagg cccataggta
     3181 ggtcccagga cctccggcaa cagcgttggc tgggaccata ttgcaatttc tttggaagac
     3241 gtttctgcag tagatgcact tgcagaaaga tgcaattccg atggctgcct tgagtcgcca
     3301 cctcgatgga caggagacgc cttttacgaa gcggtgattg ccatgccaga cggaacccgt
     3361 gtcgaagtaa cagcatgaac ttgcatgtgc tcgcatcaag tgaacgaccg gtttggtgac
     3421 cactcatgga ccgctcagga catgcttgag tccctgggtt ctgtcaggtt tcgagcaaat
     3481 caacggggaa aaatttgaca tccagatagc tgacattcgt gtgcgtccgc aactggccca
     3541 aaccgggcac tcccgatttt ggttcgactg accgctcccg ctgcttatgt atggctatac
     3601 ataagagtag agagccgaca cctacgaata cctgcttctg ggaagtgcag attcatacca
     3661 atccaagttg atggatgcac gatgcgccca aatgccgctt gctcggcgag ttgcagccga
     3721 ttgaccgagg caccacagcc atcctggcag gcacattctc aaaattgatg ttggctttgt
     3781 caccgcttgg tgactataat gtcacttatt ggtgacaaat gaaagggcaa catgaaagtc
     3841 agaacttttc ttaaagtcct gggcggcgcg atcattctcc cggctaccct gggattgccg
     3901 ggctgcagcg cagagattcc ctccgaagcc acgagtggat ggcgtggtcc cgaccccgaa
     3961 gccgacatcc gacgttgggt tttgagttat gcgttgctgg cgccacactc tcataacctt
     4021 cagtcgtggg tggcagacct tcgccaacct gacgaaattg tgcttcgttg cgatctgtct
     4081 cgtctgctgc ccgaaactga tccctacttc cggcagatca tgatgagcca tggcacattc
     4141 ctggagttgc tcgatctcgc tgcgcgtgaa cgtggctaca aaagtgacat caccttgttt
     4201 ccagaaggca gctttgatgc actgcagctc gatgcgcgac cggtggcccg cattcgtctg
     4261 ctcccggatg ccagcatcca gaaagacccg ctgttcgcac agattctgaa gcgccgaacc
     4321 aaccgtagca tctatgacgc acagcgcgca gtgccgacgc aggcttggca ggcgatgagc
     4381 aaggcgcttg gaagtgcagc ggtgctgctg gactatgtgg atcttgccaa gcctgaagcc
     4441 atggcgcagc accgcaaaat cgcatctgac gcgtggcgca ttgagatgac aacaccgcga
     4501 accatacttg agtcgtacaa ggttcttcgg gttggcgccg acgaaattct caagcaccgc
     4561 gatggcttgt ccatcatgga ccccaaactg gtgtggttag atcgcctcgg actgtttgac
     4621 cgttcgcgcg cacccgcgcc ggatgcctat gcgacgacca gccagatcaa ggaatttgat
     4681 gccaagatcg actcgactcc cgcgtttctt tgggtgaaaa ctgcgcgaaa tgaccgcgtt
     4741 gcccaaatcg cggccggtcg tgcctatgcg cgcgtgcagt tggccgccac agcccatggc
     4801 gtggcaatgc aacccttgca gcaggccttg caggaataca ccgaacagca ggccaaccat
     4861 caggccatcc accagctttg tggggcgaca ccatcaggtc aaaccgtgca gatgtgggcg
     4921 cgcgtcggct tcgcccccgc ggttcctgca gcaccacgtc gaccacttgc cgagatcctt
     4981 cgcacctagt ttttggagaa tggcatgctg aattccaggc attttgaaaa acacacttgt
     5041 ctcgctatcg cttgcaacct gggtagcctg acccgccctg tcccagcagt tttgccaatt
     5101 gcttaagccg cgttcagcaa ccatgaccaa gctgccaacc cgcaaccgcg acttgacttc
     5161 ggcaaagatc atcgaagccg ttggccgtgt gctggccagg gacggaatcg acaaaattgg
     5221 catcaacgcc attgcacgtg aagcaggcgt tgacaaggta ttgatttatc ggtatttcga
     5281 gggtctgccg aaattgctca ctgcatgggc ggcgagcggg gagttttggc caagcgttca
     5341 agaactcgtc gacatcagcc cacccaatct gatggaactg cctttggctg agcgctatgt
     5401 tttctttttt gaccacttta ttgacagtct gcgcgcccga ccactaacga ttgaaatcct
     5461 ggcgggtgaa atcagtcacc gcaatgcgct gaccgacatc atggaagatc aacgcgaaaa
     5521 atggggtgaa gaagtaggtc gcaccttggc ggcgctctca gagtatcaag cccatccagc
     5581 gctgttgccc ctgacgaatg tgttgattgc tggcgtgcaa aacctgctga tccgagcacg
     5641 ctcgattcat tctttcggtg gcctcgatat tcagtcagac gaaggatgga atctggtcaa
     5701 gaaatccatc ggcgtgatgg caaacaagtt gtttaaacca tgaacatttt tctgcgtatg
     5761 cgtcctcaaa agctacttat ccttggcggg tatgggaatg ctggtctggc cattgcccgc
     5821 ctgttggcca aggtgggtga ccttcaaatt gttttggctg gtcgccgcat tcagtgtgcg
     5881 gctaatgcag cgaggcagat ggatgccgaa tttggcaccg atcgcttctc tggcttggag
     5941 gtgaacgcag ccaatcgaga ctcactattg gctgccttca gacaggtgga tttggtcatc
     6001 gtggccgcca gcaccattga gcacaccaaa gtagtcgcag aggccgcgct ggaagccggg
     6061 attgactacc tggatgtcca gatttcggtc aaagccaaac atcaggcgct ggagtctttg
     6121 cgcgagcgta ttcgtacagc agagctttgt ttcattaccg acggtggatt tcgccctggc
     6181 atcccggccg ccatggtgcg ctacgccgct cgccagatgc cgggtttgga gtcggcggcg
     6241 gttgcgtcag cctttcaggt caattggcga gatcgggcgt tttccagttc gtcagcggta
     6301 gagtttacgg atgagctcaa gtctttcagc ccaagagtcc tgaaggatgg ggtatggcag
     6361 tcggccagtc tgcgggccta cccacaggtt gattttggcc cgccattcgg catcaagtat
     6421 tgcgcgccga tgttcatgga ggagttccgt gagttgcccg acttgatccc cacactgaga
     6481 aacaccggct tttacagcag cgggttgggt ggcttcgtgg actacatcct cattccgttg
     6541 gcgtttggcc tgcttgccct gtcacctgaa gggtcccgcc attggattgg ccgtctgatg
     6601 gagtgggggt tgaaacacac gacgcaccca ccctatgggg cggtattgca aatggacgcg
     6661 cgcgggccga agtgcgcgct tcgaatgacg gtgtctcacg cagatgccta cctggttacc
     6721 gcagcgccga cagtggcttg tctacttcaa tactttgatg gcagcctgcg caagcctggg
     6781 ctgtggaagc aggccacgct ggtggaaccg gtgcaattct ttgacgacct cacaaggctt
     6841 ggtcttgatg tcaaagtgca ggcgatggat gcgccacaac cgatgtaacc atttggcaac
     6901 aggacaaact tttacaggcc atcaaagcac caacttcagc gagggtttca aatgtttgac
     6961 atttcaaaac gcgtatttag cctttgcctg gcgctggcaa gcgcagaaat gctccatggg
     7021 atcactcgca tccgttacct ggtaccgctc gtgggcaaat ccaacgccca gaaaatcagc
     7081 atcgtcaccg gaacacttct tgccttcggt gtttgttatg tgttcattcc ggccatggga
     7141 ttgaccaccc gcttctctgc catagcgctt ggcgtttttc ttgccgcttt catggcgtcg
     7201 tttgacattg ctgtgggtcg ccttttgatg agacgcaagt gggcactcat tgctgaagat
     7261 tttgatccgc gcaaaggcaa ctatctggtc tttggccttg ccgccctggt ggcgataccc
     7321 actttcgttt tgcgaatcgc ataacgggct gctgcctaaa aggtgccaca gcatcgacac
     7381 atttaaccga ctgcggtcat cgaaatcgtc gtcacattgt cgacctccaa atgcaggtct
     7441 gaacggctgg tctggctgtg gcagttcggc aagctggctg accgggtctc tggcagccgc
     7501 aagtcacccg atgaaccagc ctgacaacag ccggtaaaaa atagcgcgca gttcgtggca
     7561 aagtgcacca cgtcaagcag tttgtaagcg ccagcaacac ccccctaact tggttccatt
     7621 gtggtgagtt gctgcgccct cgccacgcag gtcttggcgc gcctgaccca tcatcacgcg
     7681 cagcaaaggc atgcacccga actttgcccg gcgttattgt caaaaccctg cccagaacct
     7741 tcaaccgtgc aatacgtaga ttaatttgtt gaattacacg gatgaacctt cgctttgcac
     7801 aaaatcaact gtgatcagcc tgctcgatca atttccggcg gtggtgcttt tgggaccacg
     7861 tcagaccggt aattccattt ctaaatcatc catgtcgttg ttatctcgcc ttgccttgcg
     7921 tttgcaatgg ctgacgatga tgcccgcagc ctgcgctggc gtgaagcctt catcgccagc
     7981 tacctggagc gtgacattcc cgcgctgggc cccaaagtac ctgcagccac gctgcgccgc
     8041 ttgtggacca tgctggccca taaccagggg gccatgctca accagtccca actcgcgggc
     8101 gcactggcgg ccatctgacg gtcaagcgcc atgttcaccc ggatgtgttc aagatgtttg
     8161 aggcgcgtgc ctatgcaatg ggtttcggtc acgcggcagt aggggccatg gtgcgatcaa
     8221 gctaccacgc cgaccggcag gccgaccacg cgaagggttg cgcagccgcc tgaacactat
     8281 tagtttaata gctagtagcg caacacagtc aagggctgga ggcctatttc atgcaaaccc
     8341 attggcaaag ctccagtctc tggcttgatt tggcctcgct ggagcaacag tgcgacagca
     8401 tcagttgcgc ccgcgccctg gagttgtacc gcatgggcct gggcaaaagc ctgcggacgc
     8461 tggcgcacct ccagatcgaa taagacccgg gccgcctgaa cttgcccgcg ctgatcattg
     8521 cgccggtcag cctgatggcc aaagccggaa gcgcgacgac ctgatcaccc agttcaccag
     8581 cggcgcagtg cccttgtttc tgatcagcct caaagccggt ggtgtcggcc tgaacctgcc
     8641 gcaagccgac acggtgatcc attacgaccc ctggtggaac ccgg
//
LOCUS       DAOSLL010000130         8653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7635_length_8653_cov_4.194929, whole genome shotgun sequence.
ACCESSION   DAOSLL010000130 DAOSLL010000000
VERSION     DAOSLL010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8653
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7635_length_8653_cov_4.194929"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="PLB25_07595"
     CDS             <1..561
                     /locus_tag="PLB25_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE41476.1"
                     /translation="QASVVWRAAAFLPFVVSPVTVAFGLLLLYPGWTASLSLLLVAYA
                     VLAYPFVAKSMAAGLDSLPVHLGQAARTLGASRWRCFWRVTLPLLRPALRRGMAFAAA
                     TALGEFAVTLFLSRPEWATLTTLIYQRLGRPGAANLDAAMVLACVLMALALAVFLSIE
                     WPGPDRKGSAPMRLPALASTPMPQHA"
     gene            554..1198
                     /locus_tag="PLB25_07600"
     CDS             554..1198
                     /locus_tag="PLB25_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE41477.1"
                     /translation="MLELRHIHQRYADQPLLQDVSLQVAAGEIVALLGPSGSGKSTLL
                     NITAGLLAPDAGSVWFDGQNITHLAPEKRRFALMFQDFALFAHLNVQDNVAFGLIEQR
                     VGSREARARSLQMLDVFGLGDLAGRRVWNLSGGEQQRVALARALITQPRALLLDEPFS
                     ALDADLRVALRDEFRARIEVANMATLLVTHDEPEARAMAHRAVRLVAGRLHEVW"
     gene            complement(1655..2866)
                     /locus_tag="PLB25_07605"
     CDS             complement(1655..2866)
                     /locus_tag="PLB25_07605"
                     /EC_number="7.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HOE41478.1"
                     /translation="MEHLSFLSLFQGIATLVAADPAVMWGRIFLMLLGFLLIYLGTRE
                     VLEPLLMIPMGLGMAAINAGVMFLEGGKAGTLFVDPLMSDPTDLVNIMQINWLQPIYT
                     LTFSNGLIACFVFMGIGVLLDVGYVMARPFQSMFIALFAELGTILVFPFAIWLGMKPG
                     EAAAVATIGGADGPMVLFTSLILAKDLFVPITVVGYLYLGLTYGAYPYLIKWLVPERI
                     RGINMSADRGPKITRSQKMIFAVVTCTLLCLLFPVAAPLFFSLFVGVAVRESGLESFT
                     KLLSETFLYGATFFLGLTLGVLCEAGTLLDPVVLKLLLLGMLALLISALGGLAGGYIL
                     YFATGGKFNPIVGIAGVSCVPTTAKVVQKIATASNPSAIILPQALGANISGVITTAII
                     AAVYVSVLRGT"
     gene            complement(2894..3286)
                     /locus_tag="PLB25_07610"
     CDS             complement(2894..3286)
                     /locus_tag="PLB25_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41479.1"
                     /translation="MPRQFKVTVNGREYEVAVLEVTAGATSSSAQMSMPGTPAPMMAA
                     ATAAPAAAAPVAAAAGAGDEVAQMGGVVVQVDVKVGQTVAVGDKLLVLEAMKMKSHLL
                     ASRAGKVTRIMVAAGDPVEGGQPVMTIQ"
     gene            complement(3301..3630)
                     /locus_tag="PLB25_07615"
     CDS             complement(3301..3630)
                     /locus_tag="PLB25_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41480.1"
                     /translation="MVGQAIVTAFQIYGISIVIAMLVAVLIKVMVTVTGRIEQARVAT
                     EVPQGEVCPILLGIPDEDVAALSAAIFAVIGPHRILQISERNRAWASQGRAAQHSSHA
                     GHPRPQR"
     gene            complement(3655..5208)
                     /locus_tag="PLB25_07620"
     CDS             complement(3655..5208)
                     /locus_tag="PLB25_07620"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HOE41481.1"
                     /translation="MTIPIESMNTLRDRREKVLKNVSPEKLAALHARGSFSARERIDT
                     LFDDGSFQELGMHAHHNAVSFGMADRELPTDGVITGTGYIGDMQVASFSQDFQVLAGT
                     MGKMQARKIVRLMRHALRVGVPMVAFKDSGGARIQEGVDALSGYGDVFYYNVMLSGVV
                     PQIAVICGPCAGGAAYSPALMDFVIMTRHNAYMFLTGPEVIKAVTGRTATMDEVGSAE
                     MHASVSGNVHFIAENDEHAINLVKQLLTYLPPNNTEDPPHDMSVPIDEMEDQGIYDLI
                     PGSSSDPLDMHAVIRRIVDNGQLFEVHANFAQNVIIGFARICGMVIGVVGNQPMVRAG
                     ALDMDAADKIARFVRMCNVFNIPLITLVDVPGFLPGVEQERGGIIRHGAKMLHAFASC
                     TTPKITVILRKAYGGSYLAMCSQEMRADMVYAWPSAEIAVMGAEAAVKILYRKEIEQA
                     TDRKAKGAELAQDYRDKFASPYAAASNFYITDVIDPADTRWMLALALRKSLGKRELRP
                     AKKHGNMPL"
     gene            complement(5385..6698)
                     /locus_tag="PLB25_07625"
     CDS             complement(5385..6698)
                     /locus_tag="PLB25_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HOE41482.1"
                     /translation="MEFLLVVFLTLLNGVFAMSELALTVSRKARLTAMAEAGDKGALA
                     SLALMDNPNQFLSTVQVGITSIGVLNGIVGEAAFSGGVAHWLQAVGLTEKVAAISATA
                     LVVTLITFTTIIFGELVPKRIGQLHPELVSRWVARPMLWLARAAGPFVRLLSGTTAAV
                     LKMLRIDTNAARTMTEEEISASLEEGVDAGVIEEHEHQMVQNVFGLDDRPLTSLMVPR
                     AEVEWLDASFSVAQSLQKVGADGAQRAHSWYPVCRGSMDDVIGKISVAHLLELGVQTP
                     GKLEDFASVAVFVPETLSGMELLEQFRAKVGRLVLVVDEYGVVQGLLTPHDLLEAITG
                     ELQPGVKTDAWATLRDDGTWLLEGLMPVSELKARLDIEELPDEDRGRYNTLAGLLMAV
                     CGHLPATGEKIHCGNWVFEVLDLDGKRIDRVLAGPLDVVTCQQNM"
     gene            6723..7574
                     /locus_tag="PLB25_07630"
     CDS             6723..7574
                     /locus_tag="PLB25_07630"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HOE41483.1"
                     /translation="MALYLIGDVQGCDSALQRLLDAISFSPSRDTLYLLGDLVNRGPD
                     SAGVLRRLMRLGASAHCLLGNHDLHLLAAAVGARPASRKDTLSNVLDAPDRSAMLAWL
                     RQQHMASLMTHQNKDYLLVHAGVLPSWSAAKTIALAAEVEALLRSPALPDFLHQMYSD
                     KPVGWRDDLTGVDRLRVIVNALTRLRFCSADGDMEFDTKEGLNTAPPGYLPWFDVPGR
                     RTADVVVAFGHWSTLGWLDRDDVLALDSGCVWGGHLSALMLNAGSLEQPHELIQVSCE
                     QSQKPGK"
     gene            complement(7594..>8653)
                     /locus_tag="PLB25_07635"
     CDS             complement(7594..>8653)
                     /locus_tag="PLB25_07635"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE41484.1"
                     /translation="GVEILIATPGRLLDHIEAKNCVLNQVEYVVLDEADRMLDIGFLP
                     DLQRILSYLPKQRTTLLFSATFSSEIKRLASSYLQDPVTIEVARSNAAASTVEQHFYS
                     VEGDDKRHALHQILRQRALKQAFVFVNSKLGCARLARSLEREGLKTTALHGDKSQDER
                     LKALDAFKSGEVDLLVCTDVAARGLDIKDVPAVFNFDVPFNAEDYIHRIGRTGRAGQA
                     GLAVSFAARSDARLVSDIEKLLRSKIELEPIEFEENQPDIRKQGHINDGRRMYQVEES
                     GVRRDEYASRNPRTPRSDYRRSAPIAQDPFFDKPYEPTLPADAAPAWEAATTQPGVRT
                     ISSNIKSRRKVAALFSAH"
BASE COUNT         1749 a         2542 c         2556 g         1806 t
ORIGIN      
        1 caggcctcgg tggtctggcg cgctgctgcc tttttgccgt ttgtggtgtc gccggtgacg
       61 gtggcgtttg gcttgttgtt gctatacccc ggctggactg ccagcctgtc cttgttgctg
      121 gtggcctacg cggtgctggc ttacccgttt gtggccaagt cgatggcagc cgggcttgac
      181 agcctgccgg tgcatctggg gcaagcggca cgcaccctgg gggccagccg gtggcgctgc
      241 ttctggcgcg tgaccttgcc gctgctgcgt ccggccttgc gccgtggcat ggcctttgcc
      301 gctgctacgg ctttggggga atttgccgtc acgctgtttt tgtcgcgccc tgaatgggct
      361 acgctgacca ccttgattta ccagcgcctg ggtcgccctg gcgcggccaa tctggatgcc
      421 gccatggtgc tggcctgtgt tttgatggcg ctggcgctgg cggttttttt gtcaattgaa
      481 tggccaggac ctgaccgtaa ggggagtgcc ccgatgcggc tgccagcact ggccagcacc
      541 ccgatgccgc aacatgcttg agcttcgcca cattcaccag cgctatgcag atcaacccct
      601 gctgcaagat gtcagtttgc aggtggcggc cggggaaatt gtggccctgc taggtccgtc
      661 tggcagcggc aagagcacct tgctcaacat aaccgccggc ttgctggccc ctgatgcggg
      721 cagcgtctgg ttcgacggcc agaacatcac ccatctggca cccgagaagc gccgttttgc
      781 cttgatgttt caggactttg ccctgtttgc acacctgaat gtgcaagaca acgtggcttt
      841 tggcttgatc gagcaacgcg tcggcagccg cgaggccagg gcacggtcgc tgcagatgct
      901 ggatgttttc ggactgggcg acctggccgg gcgccgcgtc tggaatcttt caggcggcga
      961 gcagcagcgg gtggcgctgg cccgggcgct gatcacgcag ccgcgcgcgc tgttgctcga
     1021 tgagccgttt tcagcgctcg atgccgacct gcgggtggcc ctgcgtgatg agtttcgtgc
     1081 gcgcatcgag gtggcgaaca tggcaaccct gctggtcacc catgatgagc cagaagcccg
     1141 agccatggca caccgggcgg tgcggctggt ggcagggcgg ctgcatgagg tgtggtgacg
     1201 cgcattaact tgcttccagt tggacgcacc agcggctaaa cccatgccag acgagctgga
     1261 atcgaacagc cttcgtcgca acgaagcgac tcccatggaa catctgaagt agtttgagtt
     1321 tgtgggagtt gcgccctcgc gacgaatgtc gctatgactt atcagtaatt cttcccggga
     1381 atgcgaaaga cttgactggt tggtgaccat atccctcaat tggacgctcc gtcaccccag
     1441 cggtgaacgt gcacggagcc gggcactgaa gttgatgata gcccgatcct gtcacaaatg
     1501 acagtggcgg cgctgcccga agacagtgcc gccacttggc cgcaagctgg ttaacccagt
     1561 aacaatctga cttacccggt agcgactgac atttagctac aaagtatata gctgtcagcg
     1621 cttatcccat aagggctaga tgccaatttc ctgactaagt cccgcgcagc acactgacat
     1681 acaccgccgc gatgatggcg gtggtgatga caccgctgat gttggcgccg agcgcttgcg
     1741 gcaggatgat ggcacttggg ttggaggctg tggcaatctt ttgcaccact ttggcggtag
     1801 tcggaacgca actgacacca gcaatgccga cgatcgggtt gaacttgcca ccggtagcga
     1861 aatagaggat gtagccaccg gccagacctc cgagcgccga gatcagcaag gccagcatgc
     1921 ccagcagcag cagtttgagc acgacggggt caagcagcgt gccggcctcg cacaatacac
     1981 cgagtgtcag acccagaaag aaggtggcac cgtacaggaa ggtctcgcta agcagcttgg
     2041 taaaagactc caggccactt tcccgcactg ccaccccgac aaacagcgag aaaaacagcg
     2101 gtgctgccac cggaaacaac aggcacagca gcgtgcaggt caccacggca aaaatcatct
     2161 tttgcgaccg ggtgattttt ggcccacggt cagcagacat gttgatgccg cgaatccgtt
     2221 caggaaccag ccacttgatc agatacgggt atgcgccgta ggtcaaaccc aggtagaggt
     2281 aacccaccac ggtaatcggt acaaacaggt ctttggccag aatcagcgaa gtaaacagca
     2341 ccatcgggcc atcagcgccg ccaatcgttg ccaccgcagc agcctcacct ggtttcatgc
     2401 ccagccagat ggcaaacggg aacaccagaa ttgttcccaa ttcggcaaac agcgcaatga
     2461 acatgctctg gaatggccgt gccatcacat agccaacatc cagcaacaca ccaataccca
     2521 tgaaaacaaa acaggcgatc aggccgttgc tgaaagtcag ggtgtaaatc ggctgcagcc
     2581 agttgatctg catgatgttg accagatcgg tcgggtcaga catcagcgga tcaacaaaca
     2641 gcgtgccagc tttaccccct tccagaaaca tcacgccagc gttgatggcg gccatgccca
     2701 gacccatcgg gatcatcagc agcggctcaa gtacctcgcg ggtacccagg tagatcagca
     2761 aaaagccgag cagcatcaaa aaaatgcggc cccacatcac cgcagggtcg gctgcaacca
     2821 gggtcgcaat gccctggaac aggctaagaa agcttaagtg ttccatagtg tcaggtgctt
     2881 gggtgctgac ggcttactgg atggtcatca ccggctgacc accttcgacg gggtcaccgg
     2941 ctgccaccat gatgcgtgtc accttgccgg cccggctggc cagcaggtgg cttttcattt
     3001 tcatggcttc gagcaccagc agtttgtcac cgacggccac ggtttggccc accttgacat
     3061 cgacctgcac caccacgccg cccatctggg cgacttcgtc gccagcacct gcggctgctg
     3121 ccactggtgc tgcagcggcc ggggctgctg ttgcggcggc catcatcggc gccggggtgc
     3181 ctggcatgga catttgcgct gaagaagaag tggcaccggc ggtgacttcg aggacggcta
     3241 cttcgtattc gcggccgttg acggttactt tgaattgacg gggcatgatg aattcctgtt
     3301 ttaacgttga ggccgtgggt gtccggcgtg ggatgagtgt tgagcggccc gaccctggct
     3361 ggcccaggcg cggtttcttt cggaaatctg caggatgcga tgcgggccga tgacggcaaa
     3421 aatggcggca gacagggctg ccacatcttc gtcgggtatg cccagcagga ttgggcagac
     3481 ttcgccctgg ggcacttcgg tggcgaccct ggcttgctcg attctgccgg tgacagtgac
     3541 catcaccttg atgagcaccg ccaccagcat ggcgatcacg atggaaatac cgtaaatttg
     3601 aaacgcggta acgattgctt ggccgaccat gttgtgtgtc cttggtaaaa gcgcttacag
     3661 cggcatgttg ccgtgcttct tggccggacg cagttcgcgc ttgccgagcg atttgcgcaa
     3721 agccagcgcc agcatccagc gggtgtcagc cggatcaatc acatcggtga tgtagaagtt
     3781 ggacgctgca gcatagggcg aggcaaactt gtcacggtaa tcctgcgcca actcggcccc
     3841 tttggccttg cggtcggtgg cttgctcaat ttccttgcgg tagagaatct tgacggctgc
     3901 ttcggcaccc atcacggcaa tctcggcact tggccaggca taaaccatgt cagcgcgcat
     3961 ttcctggctg cacatcgcca gataggagcc gccgtaggct ttgcgcaaaa tcaccgtgat
     4021 cttgggtgtg gtgcaggacg caaaggcatg cagcatcttg gcaccgtggc ggatgatgcc
     4081 gccgcgctct tgctcaacgc cgggcaggaa gccgggcaca tcaaccaggg tgatcagggg
     4141 tatgttgaac acgttgcaca tgcgcacaaa gcgggcaatc ttgtcagcag cgtccatgtc
     4201 gagtgcgcca gctctgacca tcggctggtt gcccacgaca ccgatcacca tgccgcaaat
     4261 gcgggcaaag ccaataatca cgttttgcgc aaaattggca tgcacctcga acaactggcc
     4321 gttgtcaacg atgcggcgaa tgaccgcatg catgtccagc ggatctgacg aactgccagg
     4381 gatcaggtcg taaatgccct ggtcttccat ctcgtcaatc ggaaccgaca tgtcgtgcgg
     4441 cggatcttcg gtgttgttgg gcggcagata agtcagcagt tgcttgacca ggttgatggc
     4501 gtgctcatca ttttcagcaa tgaagtgaac attgccgctg accgaggcgt gcatctcggc
     4561 actgccgact tcgtccatgg tggcggtacg cccggtcact gccttgatga cctcggggcc
     4621 ggtcaggaac atgtaggcgt tatgccgggt catgatgaca aagtccatca aggctggcga
     4681 ataggcggca ccaccggcgc aggggccaca aatcacggcg atttgcggca ccacgccgga
     4741 caacatgacg ttgtagtaaa aaacgtcgcc atagccagac aatgcgtcaa cgccttcctg
     4801 gatgcgggca ccgccggaat ccttgaaggc caccatcggt acgccaaccc gcaaggcgtg
     4861 gcgcatcagg cggacaattt tgcgcgcctg cattttgccc atggtgccag ccagcacctg
     4921 gaagtcctgg ctgaaggagg ccacctgcat gtcgccgata tagccggtgc cggtgatgac
     4981 accgtcggtg ggcagctcgc gatccgccat gccaaaagac accgcattgt ggtgcgcatg
     5041 catgccgagt tcctgaaaac tgccatcgtc aaacaaggtg tcgatgcgct cacgggccga
     5101 gaacgagccc ctggcgtgca gtgctgccag cttttcaggg ctgacgtttt tgagaacctt
     5161 ttcgcgccga tcgcgcaatg tgttcatgga ttcaataggg atggtcatct catttcctgt
     5221 tcaagtactt gatctacctc aaaccatacc acacaaaaag caggtaattc cgaccggaac
     5281 tggctacaca tcttgccgcg ttactgtata ggcggcgagg catcaaaata ctgcttttcc
     5341 tagcagattt gggatttaaa tttatccgga tttatcctga taagtcacat attttgttgg
     5401 caagttacaa catccagtgg acctgccagc accctgtcaa tgcgcttgcc atcgaggtca
     5461 agtacttcaa aaacccagtt gccacagtgg attttttcgc ctgtggctgg caggtgtcca
     5521 cagaccgcca tcagcaatcc ggccagcgtg ttgtagcggc cccggtcttc gtcgggcagt
     5581 tcctcaatgt ccaaccgagc cttgagttca ctgactggca tcaggccttc cagcaaccag
     5641 gtgccgtcgt cgcgtagcgt cgcccaggca tcggtcttga caccgggttg cagttcgccg
     5701 gtaatcgctt cgagcaggtc gtgcggggtc agcagaccct gcaccacgcc atattcgtcc
     5761 accaccagca ccagtcggcc caccttggcg cgaaactgct ccagcagctc catgccgctg
     5821 agcgtttcag gcacaaaaac cgcgacgctg gcaaagtctt cgagtttgcc gggcgtctgc
     5881 acccccagtt ccagcaggtg cgcaacgctg attttgccga tcacgtcgtc catcgacccg
     5941 cggcagaccg gataccagga atgcgcccgc tgggcgccat cagcccccac tttttgcaga
     6001 ctttgggcca cgctgaagct ggcatcaagc cactccactt ccgcccttgg caccatcagc
     6061 gatgtcaggg gccggtcgtc cagaccaaac acgttttgca ccatctggtg ctcatgttct
     6121 tcgatcacac cggcatcaac gccttcttcc aggctggcgg agatttcttc ttcggtcatg
     6181 gtgcgcgcgg cgttggtgtc aatgcgcagc attttcagaa cggcagcggt ggtgccggac
     6241 agcagcctga caaacggtcc ggcggctcgt gccagccaca gcatcggacg ggccacccag
     6301 cgcgatacca gttcggggtg caactggcca atgcgtttgg gcaccagttc accaaaaatg
     6361 atggtggtga aggtgatcag ggtgaccacc agcgccgtgg ccgagatcgc agccaccttt
     6421 tccgtcaggc caactgcctg cagccagtgt gctaccccgc cgctgaaggc agcttcgccg
     6481 acaatgccat tgagcacgcc aatcgaggtg atgcccactt gcacggttga caagaactga
     6541 ttggggttgt ccatcaaagc cagcgaagcc agggcaccct tgtcaccggc ttctgccatg
     6601 gcggtgagtc gtgctttgcg gctgacagtc agtgccaatt cggacatggc aaaaacgcca
     6661 ttgagcagcg tcaaaaaaac gaccagcaag aattccattc gacctcagcg agaataaaca
     6721 ccatggcact ttacttgatt ggcgacgtgc agggctgtga cagtgccttg cagcgtctgt
     6781 tagacgcaat ttctttctcg cctagccgcg ataccctgta cctgctgggt gacttggtca
     6841 accgggggcc ggactcggca ggcgtgttac gccgcctgat gcgcctgggt gcatcagccc
     6901 attgcctgct gggcaatcat gacctgcatt tgctggctgc cgctgttggg gcgcgcccag
     6961 ccagccgcaa agacaccctg agcaatgtac ttgatgcgcc ggaccgatct gccatgttgg
     7021 cctggttgcg tcagcagcac atggcgagcc tgatgacgca ccagaacaaa gactatttgc
     7081 tggtgcacgc cggtgtcctg cctagctgga gtgccgcaaa aacaatagcc cttgcggctg
     7141 aagttgaagc gctattgcgc agccccgcct tgcccgactt tttgcaccag atgtacagcg
     7201 acaaaccggt cggctggcgc gacgatctga ccggtgttga tcggctgcgg gtgatcgtca
     7261 atgcgctgac ccgcctgcgc ttttgcagcg cagatggcga catggaattc gacaccaaag
     7321 aaggcctgaa caccgcaccg cccggctatt tgccctggtt tgacgtgccc ggtcgccgga
     7381 ctgccgatgt cgttgtggcc tttggccact ggtcaacgct gggttggctg gatcgcgatg
     7441 acgtgctggc gctcgattcc ggctgtgtct ggggtggtca cttgagcgcg ttgatgctca
     7501 atgcaggtag ccttgagcag ccgcatgagc tgattcaggt tagctgcgag cagtcacaaa
     7561 aacccggcaa gtagccgcag gcactgggtg ccgtcaatgg gcgctgaaca gcgctgccac
     7621 cttgcgtctg gatttgatat tgctggaaat ggtgcgcaca cccggctgcg ttgtggcggc
     7681 ttcccaggcc ggtgcggcat ctgcgggcag ggttggctca taaggcttgt caaaaaacgg
     7741 gtcctgtgcg atgggtgcac tgcgtcggta gtcactgcgc ggcgttcgtg gattgcggct
     7801 tgcatattcg tcacggcgca caccactttc ttcaacctgg tacatccgcc gaccgtcgtt
     7861 gatatgacct tgcttgcgaa tatcgggctg attttcttca aactcgatag gttcaagttc
     7921 aatcttgctt ctgagcagtt tttcgatgtc cgacaccagc ctggcatcgc ttctggcggc
     7981 aaaactgacg gccagaccgg cctggccagc gcgtccggtg cgtccgatgc gatggatata
     8041 gtcttcggcg ttgaacggca catcaaagtt gaacacggcg ggcacgtcct tgatgtcgag
     8101 gccacgtgcc gcgacatcgg tacagaccag caggtcaacc tcgccgctct tgaatgcgtc
     8161 gagcgccttg agacgctcgt cctggctttt gtcaccatgc agtgcggtgg ttttcagtcc
     8221 ttcacgctca agcgaacgcg ccagccgggc acagccgagt ttgctgttga caaacacaaa
     8281 cgcctgtttc aaggcacgtt ggcgcaagat ctggtgcagc gcatggcgct tgtcatcacc
     8341 ctcgacgctg taaaaatgct gctccacggt ggacgcagct gcattggagc gggccacttc
     8401 aatcgtcacc gggtcttgca ggtagctgct ggccagacgc ttgatctcgc ttgaaaaggt
     8461 ggccgaaaac agcagcgtgg tgcgttgttt gggcaggtag ctcagaattc gctgcaaatc
     8521 tggcaaaaaa ccgatgtcca gcatgcggtc agcttcgtcg agcaccacat actcaacctg
     8581 attgagcacg cagttcttgg cctcgatgtg gtccagcagc cgacccggcg tggcgatcag
     8641 gatctcgacg ccc
//
LOCUS       DAOSLL010000131         8632 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7650_length_8632_cov_4.684855, whole genome shotgun sequence.
ACCESSION   DAOSLL010000131 DAOSLL010000000
VERSION     DAOSLL010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8632
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7650_length_8632_cov_4.684855"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(56..1888)
                     /locus_tag="PLB25_07640"
     CDS             complement(56..1888)
                     /locus_tag="PLB25_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE41485.1"
                     /translation="MNLQNLRLASKLWLSSTLIVLGILLVVSYTAVRSANDRAESTVT
                     LDKLNNRVKLALQWAGLTQTNAARSQAVLLSADPAVEAGLKAAIADTSAQIGTIQKSI
                     ESEDLTGADRNQLETIAGHRKRVLETRAVAMKQRADGLPAELATTVTTLYQPAMEAYQ
                     KSQRDFVTLQEQAFEATRASYVQRSKELVVVASGLMLLMVSGLLAGVSWLIRSIRQPL
                     VTASDLAARIAQGDLSMQIDTSRTDEFGELMKSLASMNASLAKMVSQVRQSTDSIAIA
                     SGEIAAGNNDLAQRTEHTSGNLQSTASSMGNLMQTVQISADNARQASTLAAAASSVAE
                     RGGQVVRQVVSTMEDINASSRKIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVASEVRSLAQRSAEAAKEIKMLINTSVDKVASGTQLVSDAGVTMNDIVQSVRQVAE
                     VIGHITAASGDQSAGISDINQAIGDLDQMTQQNAALVEQSAAAAASLRDQADQLARAV
                     AVFKIGNQMQALPQRPSRDITPRPSQLSYQRPAQLARPKASALPAAAAPKRLGTAPKA
                     IAAPRPAMAKPMAPKASPVKPAAPKASAAKPAPAKTGDDDWETF"
     gene            complement(1991..2497)
                     /gene="folK"
                     /locus_tag="PLB25_07645"
     CDS             complement(1991..2497)
                     /gene="folK"
                     /locus_tag="PLB25_07645"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HOE41486.1"
                     /translation="MTQQQVSTTPEDGPAPVLAYVALGANLGDARQTVLWAISQFDGL
                     ADVELVARSHLYRTEPVDASGPDFINAVVAVMTRLSAHALLHQLQHLEQLAGRQRPYR
                     HAPRTLDLDLLLYGNASISSADLMVPHPRMYQRAFVLVPLAEIAPQRVATGHLRGVQS
                     QRIECLAV"
     gene            complement(2527..4119)
                     /gene="pcnB"
                     /locus_tag="PLB25_07650"
     CDS             complement(2527..4119)
                     /gene="pcnB"
                     /locus_tag="PLB25_07650"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004652 - polynucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HOE41487.1"
                     /translation="MIKKFINKLLRRATPAASSPFGKREEIGPEVHGINPQLVDERAL
                     GVLRTLKGAGFEAFIVGGAVRDLMLGLAPKDFDVATNATPEQVKALFRRAFIIGRRFR
                     IVHVVYGRGREHEVIEVSTFRAYLDNAAAEQVAGNEKTSKSDLVGMKHAVDSTGRVLR
                     DNVWGPQEEDAVRRDFTINAMYYDPETQIVVDYHHGMRDAKAHVIRMIGDAATRYRED
                     PVRLIRAVRFAAKLNALGFKLDPKTATPLIKSRELLAEVPQSRLFDEMLKLLQTGHSL
                     ASIEQLKLLGMAQGIYPLLDVVVERADTPFVRSALQDTDRRVGEGKPVAPSFLLACVL
                     WSDVRDGWTQRIKNQQPTHPALQDAIEDVFHARIGDVSGRGKLAGDMRDIWLMQPRFD
                     KRVGSAPFGLVEQPRFRAAFDFMRLRAESGELDEVLADWWEEFSLADDNLRNDMVDEV
                     RLEQQQRGRPPRAPRVHKAPVAAGVNLPLMAEPDQGAPSESGQFDVIAAPEEGTQLPK
                     KRRRRRRSSRGQGDAPPAGPQT"
     gene            complement(4173..4841)
                     /locus_tag="PLB25_07655"
     CDS             complement(4173..4841)
                     /locus_tag="PLB25_07655"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOE41488.1"
                     /translation="MKLALFDLDDTLIPIDSDYEWGVFTTALGWNDPTEFSHRNDAFY
                     AQYKAGTMNIWEYIRFATEPLCRYGAIESEAAHALFMRDVVQKVIQPEALALVRQHQA
                     AGDAVIIVTATNEFVTRSIAQIFGVTDLMAVNLARDPVSGWFNGEILGTPSFREGKVT
                     RVGQWLAERQLDWQDVDSTFYSDSINDLPLLERVNHPVATNPDAQLRAIAQARGWRIL
                     ELFQ"
     gene            complement(4857..5546)
                     /gene="hda"
                     /locus_tag="PLB25_07660"
     CDS             complement(4857..5546)
                     /gene="hda"
                     /locus_tag="PLB25_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HOE41489.1"
                     /translation="MKQLVLDLGLAAGPSLNNFCIGRNAALVGHLKLWLGQTGSGARP
                     GSSVPIYFWGTAGSGKTHLLNAVRLALLAQGQQVGWLDAAVVPAAAFDAGWSAVLMDD
                     VQAFDAARQQVAFNWFVNAQTQQIDVLATGSLPPTDLKLRDDLRTRLGWGHVFALQAL
                     DEPQRRDVLRQNAAARGVLLSDEVMDFMLTRFSRDLASLMELLDLMDAYALQTQRAIT
                     IPLIKTMMDNA"
     gene            complement(5543..5935)
                     /locus_tag="PLB25_07665"
     CDS             complement(5543..5935)
                     /locus_tag="PLB25_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41490.1"
                     /translation="MNIDFFSFDGMPPSGDHPIEPVATRPSRYALRYRARAARAIRHH
                     ALATRLNRYGCRCALPVGRAEMRLSARCVVAPRRLLQPAGRSHATSLACFQPSNMAQA
                     GLEPSLSLCPDAPPAARSGAPNSRHPGA"
     gene            complement(5975..7066)
                     /locus_tag="PLB25_07670"
     CDS             complement(5975..7066)
                     /locus_tag="PLB25_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOE41491.1"
                     /translation="MQFTFTQKRVGAWIALLLALVVALWLLAPVLTPFVVAAILAYAL
                     TPVVDWLNRLGGNRLPRVLAVALVEIVFLLLLLALLLLVVPILVNQLPLLREQVPPLL
                     DRLNSFLQPFLTQFGLHMSLDLAGLKAFATEHLSANFQDTLGRLLASAKLGGSVALSV
                     LGNAVLIPVVLFYLLMDWQRFINLLLALVPPRLRVAVDSFTREADGVLGQYLRGQLLV
                     MLVLAVYYSAGLALFGLNLALPIGVFTGLAVAIPYVGFGLGLVMALLAGSLEFAALSG
                     HVSALVMVAVVYGLGQIIESFFLTPRLVGERIGLHPLAVIFALLAFGQLFGFVGVLMA
                     LPLSAVLLVAMRRLRAAYLGSALYRDAAS"
     gene            7226..8278
                     /gene="purM"
                     /locus_tag="PLB25_07675"
     CDS             7226..8278
                     /gene="purM"
                     /locus_tag="PLB25_07675"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HOE41492.1"
                     /translation="MTSSPTPPVALSYKDSGVDIDAGDALVERIKPFARKTMREGVLA
                     GIGGFGALFELPKRYQEPVLVSGTDGVGTKLKLAFDWQMHDTVGIDLVAMSVNDVLVQ
                     GAKPLFFLDYFACGKLDVDTAAAVIAGIARGCELSGCALIGGETAEMPGMYPAGEYDL
                     AGFAVGAVEKSKILTGADVRPGDVVLGLASSGVHSNGFSLVRKCIERAQATDTIPDTL
                     DGKPFRHAVMEPTRLYVKNLLAALAAHPIKALAHITGGGLLENIPRVLPEGTSAHLKK
                     GSWPQTELFAWLQKTAGIDDFEMNRTFNNGIGMVLVIDATEAASCAATLRSAGEVVYE
                     IGVIAASDEAARVLVR"
BASE COUNT         1740 a         2614 c         2595 g         1683 t
ORIGIN      
        1 atgactttga atgtttgaat tcttcgcatg gtatgcaaag acttgacttg ttagactaaa
       61 acgtttccca gtcatcatcg cctgtcttgg ccggtgcggg cttggccgca ctggctttgg
      121 gcgctgccgg tttgacggga ctggctttgg gtgccatcgg cttggccatg gcaggccgcg
      181 gtgcagcaat ggctttgggg gctgtgccca acctctttgg cgctgctgct gcaggcaacg
      241 cgcttgcctt gggccgggca agttgggccg gtcgctgata ggacagttga ctgggacggg
      301 gtgtgatgtc gcggctaggg cgctgcggca aggcctgcat ttggttgccg atcttgaaaa
      361 ccgccacggc acgcgccagt tggtcggcct gatcgcgcag gcttgctgcg gcggctgcgc
      421 tttgctcaac cagcgcagca ttttgctgcg tcatttgatc caggtcgcca atggcctgat
      481 tgatgtcgga aatgcccgca ctctgatcgc ccgaagcagc ggtgatatga ccaatcacct
      541 cggccacctg gcgcaccgat tgcacgatgt cgttcatggt gacaccggca tcggacacca
      601 gttgcgtgcc ggacgcaacc ttgtccaccg aggtgttgat cagcatcttg atttccttgg
      661 ctgcttcggc gctgcgctgg gccaggctgc gcacttcgct ggccaccact gcaaagcccc
      721 ggccctgctc accggcacgg gccgcttcaa cagcggcatt caaggccaga atgttggtct
      781 gaaacgcaat gccgtcaatc acgccaatga tgtcggcaat cttgcggctg ctggcattga
      841 tgtcttccat ggtgctgacc acctggcgca ccacctggcc gccgcgctcg gccaccgatg
      901 aagccgcggc agccagtgtg ctggcctggc gcgcattgtc ggcgctgatc tgaacggttt
      961 gcatcaggtt gcccatgctt gatgccgtgg actgcagatt gccggaggtg tgctcggtgc
     1021 gctgcgccag gtcgttgttg ccagccgcaa tttcgccgct ggcgatggca atgctgtcgg
     1081 tgctttggcg cacctgactg accatcttcg caagcgacgc attcatgctg gccagtgatt
     1141 tcatcaattc accaaattca tcagtgcggg aagtgtcgat ctgcatgctg aggtcgccct
     1201 gggcgattct ggcggccaga tcgctggcag tcaccagcgg ctgccggatc gagcgaatca
     1261 gccaactgac gcctgccaaa aggcctgaca ccattagcag catcaggcca ctggcgacaa
     1321 cgaccagttc tttggaacgc tggacgtagg acgcgcgggt ggcttcaaaa gcctgttcct
     1381 gcagggtgac gaaatcgcgc tgggacttct ggtaagcctc catggcaggc tggtatagcg
     1441 tcgtcacagt ggtggccagc tcggcgggca agccatcggc acgctgcttc atcgccactg
     1501 cgcgggtttc cagcacccgt ttgcgatgtc cggcgatggt ttcaagctgg ttacggtcgg
     1561 caccggtcaa atcttctgac tcgatgcttt tttgaatggt gccaatctgg gccgaggtat
     1621 cggcgatagc ggctttcaga ccagcctcaa cggccgggtc ggcgctgagc agcaccgcct
     1681 gcgacctggc ggcattggtt tgtgtcaaac cggcccactg gagagccagt ttgacccggt
     1741 tgttcagttt gtccaaagtc accgtactct cggcgcggtc gttggcggag cgcactgcgg
     1801 tgtaactgac caccagcagg atacccagca cgatcagggt ggacgacagc cacagcttgg
     1861 aagcaaggcg aaggttttgc aggttcatga acaactccgg tcaacaaggc tactgggcaa
     1921 atcatgcaca gcactggatc ttagcgcgtc accaaccccg cttcggctgg tgttctggct
     1981 gtttgggcgt ttaaaccgca aggcactcaa tccgttgtga ctgtacgccc cgcaaatgcc
     2041 cggtcgccac ccgctgtgga gcgatttccg ccaacggcac cagtacaaat gcacgctggt
     2101 acatgcgcgg gtgcggcacc atcaagtcag cactgctgat gctggcattg ccatacagca
     2161 gcaggtcaag gtccagcgtt cggggggcat ggcggtaggg ccgctggcga cccgccaatt
     2221 gctccagatg ttgaagctgg tgcagcaacg catgggcgct caggcgggtc atcacagcca
     2281 ccaccgcgtt gatgaaatcg gggccgctgg catcgaccgg ctcggtgcgg tacaaatggg
     2341 agcgcgccac cagttcaacg tcggccaggc catcaaactg gctgatcgcc cacaacacgg
     2401 tctgccgcgc atcacccaga ttggcaccca gggccacgta ggccagcacc ggtgcgggcc
     2461 cgtcttcagg cgtggttgaa acctgttgct gcgtcacagg gtcggcggtg cgatccagac
     2521 gccgattcag gtctgagggc ctgccggtgg tgcatcaccc tgcccgcggc tggaacgacg
     2581 cctgcgacgg cgttttttcg gtaactgcgt gccttcttcg ggcgcggcga tcacatcaaa
     2641 ttggcccgac tctgagggtg cgccctggtc aggctctgcc atcagaggca gattgactcc
     2701 cgccgcaact ggggccttgt gcacacgcgg tgcccgtggt ggccgaccac gctgctgctg
     2761 ctccagccgg acttcatcaa ccatgtcgtt gcgcaggttg tcgtcagcca ggctgaactc
     2821 ttcccaccag tcggccagca cttcgtcgag ctcgccgctt tctgcccgca ggcgcatgaa
     2881 atcaaacgcc gcccgaaaac ggggttgttc caccaggcca aacggcgcgc tgccgacacg
     2941 cttgtcgaaa cgcggctgca tcagccagat gtcgcgcatg tcaccggcca gcttgccgcg
     3001 tccggagaca tcgccgatgc gggcatggaa cacgtcttca atcgcatcct gcagcgccgg
     3061 gtgcgtcggc tgctggtttt tgatgcgctg ggtccagcca tcacgcacgt ccgaccacag
     3121 cacacaggcc agcaaaaaac tcggtgccac cggtttgcct tcaccaaccc ggcgatccgt
     3181 gtcctgcagg gccgacctga caaagggcgt atcggcccgc tccaccacca cgtcgagcaa
     3241 cgggtaaatg ccctgcgcca tgcccaacaa cttgagctgc tcaatggaag ccagcgaatg
     3301 gccggtttgc agcagcttga gcatttcgtc aaacaggcgg ctttgcggca cttcggccag
     3361 caactcacgc gacttgatca gcggcgtagc ggtttttggg tcaagcttga aacccagagc
     3421 gttgagcttg gcggcaaaac gcaccgcccg aatgaggcgc accgggtctt cgcggtaacg
     3481 tgtggcagcg tcgccaatca tgcggatgac atgtgccttg gcatcgcgca tgccgtggtg
     3541 gtagtcaacc acgatctgcg tctcagggtc gtaatacatc gcattgatgg tgaagtcgcg
     3601 gcgcactgca tcttcttcct gcgggcccca gacgttgtcg cgcagcacac gcccggtcga
     3661 atcgacggcg tgcttcatgc ccaccagatc acttttgctg gttttttcgt tgcccgccac
     3721 ctgttccgca gcggcgttgt ccaggtaggc gcgaaaggta gacacctcaa tcacctcatg
     3781 ttcgcggccg cgtccataga ccacgtgaac gatgcgaaag cgtcgcccga tgatgaaggc
     3841 gcggcgaaac agggctttga cctgctcagg cgtggcgttg gtggccacat caaagtcttt
     3901 tggggccagg cccagcatca gatcacgcac cgcgccaccg acgatgaaag cctcaaaacc
     3961 ggcacctttg agcgtgcgca gcacgccaag cgcacgctca tcgaccagtt gcgggttgat
     4021 gccgtgcacc tcggggccaa tttcttcacg tttgccaaac gggctgctgg cggctggagt
     4081 ggctctgcga agcagcttgt tgatgaattt cttgatcatt ttttgttggt ccaggaaggc
     4141 tgggcttgag agccatgcca acccggcaat ccctattgaa agagctcaag tatgcgccag
     4201 ccacgggctt gcgcgatggc gcgcagttgc gcgtccggat tggtcgccac cgggtggttg
     4261 acccgctcaa gcaacggcag gtcgttgatg gagtcagagt aaaaagtgct gtcaacgtcc
     4321 tgccaatcga gttgccgctc ggccagccac tgcccgacgc gcgtgacttt gccttcgcga
     4381 aacgacggcg tacccaggat ttcgccattg aaccagccgc tcaccgggtc gcgcgccaga
     4441 ttgacagcca tcaggtcggt gacaccgaaa atctgcgcaa tcgagcgggt cacgaattca
     4501 ttggtcgccg tgacgatgat caccgcatcg cccgccgcct ggtgctgccg aaccagcgcc
     4561 agcgcctcag gttgaatgac tttttgcact acatcgcgca tgaacagtgc gtgagcagct
     4621 tctgattcta tagcgccata acggcacaaa ggttcggtgg caaagcggat gtactcccat
     4681 atgttcatgg tgccagcctt gtactgggcg taaaacgcat cgttgcggtg gctgaactcg
     4741 gtcgggtcgt tccagcccag cgcggtggtg aatacacccc actcgtaatc ggagtcgatt
     4801 ggaatcaggg tgtcgtccag gtcaaaaagg gccagtttca tgcgttattg atcgctttac
     4861 gcgttgtcca tcatggtttt aatgagcgga atggtgattg cacgctgggt ttgcaaggcg
     4921 taagcatcca tcaggtcgag cagttccatc aggcttgcca ggtcccggct aaagcgcgtc
     4981 agcatgaaat ccatcacttc atcgctgagc aacacgccgc gtgcagcagc attttgccgc
     5041 aacacatcac gccgctgggg ttcatcaagc gcctgcagcg caaatacatg gccccagccc
     5101 agccgggtgc gcaagtcgtc gcgcaatttc aggtcggtcg gtggcaggct acccgttgcc
     5161 agcacgtcga tttgttgcgt ttgggcattg acaaaccaat tgaaagccac ctgctgacgg
     5221 gccgcatcga aggcctgcac atcgtccatc aacacagccg accaacctgc gtcaaaagcg
     5281 gcagcgggca ccacagcggc atcgagccag ccgacctgct gcccttgcgc caacagtgcc
     5341 aagcgaacag cgttgagcaa atgtgtcttg ccactgccag cggtgcccca gaagtaaatg
     5401 ggaactgacg aaccgggcct cgcgccagaa ccggtctggc ccagccagag cttgaggtgg
     5461 ccgaccaggg cagcgttacg cccaatgcaa aaattattca ggctgggccc agcggccagc
     5521 cccaggtcga gcaccagttg cttcatgctc ccggatgcct tgagttaggc gcgcccgagc
     5581 gggccgctgg cggcgcgtct gggcagagcg aaagcgacgg ctccaaacct gcctgcgcca
     5641 tgttggacgg ctgaaagcag gccaagctgg tggcatggct gcgccccgct ggttgcagca
     5701 agcggcgcgg ggcgacgacg cagcgggcgc ttaagcgcat ctcggcccgg ccaacgggca
     5761 aggcgcaacg gcagccgtaa cgattcaggc gtgttgccag agcatggtgt cggatagctc
     5821 gcgcagctct cgcgcggtaa cgcaaggcgt aacggctcgg tcgtgttgcc actggctcaa
     5881 tcggatggtc acccgatggg ggcatcccat cgaagctgaa aaaatcaata ttcatgagga
     5941 cttaccgatg aaaacaggcg gtcaacgcag gaatctagga tgctgcgtcc cggtacaacg
     6001 cactgccaag gtaagcagcc ctgagtcggc gcatcgccac cagcagcacg gcactgagtg
     6061 gcaatgccat cagcacgccg acaaagccaa acaactgccc aaatgccagc agcgcaaaaa
     6121 tcactgccag cgggtgcaag ccaatgcgct caccgaccag ccggggcgtc aaaaagaaac
     6181 tctcgatgat ctggcccagg ccgtaaacca ccgccaccat caccagcgcg ctgacatggc
     6241 ccgacagggc ggcaaactcc agcgaccccg ccagcaatgc catgaccagg cccaagccaa
     6301 agccgacata ggggatggcc accgccagac cggtaaagac accaatcggc aaggccaaat
     6361 tcagcccgaa cagcgccaac ccggcgctgt aatagaccgc cagcaccagc atcaccagca
     6421 attgaccacg caggtactgg ccgagcacgc catcggcttc ccgggtgaaa ctgtcaacgg
     6481 ccacgcgcag ccggggtggc accagtgcca gcagcaggtt gatgaagcgc tgccagtcca
     6541 tcagcaggta aaacagcacc accggaatca acaccgcgtt gcccagcacg gacagggcca
     6601 cgctgccgcc gagcttggct gaagccagca agcggcccag agtgtcctgg aagttggccg
     6661 acaggtgctc ggtggcaaag gccttgagtc cggccaggtc cagcgacatg tgcaggccaa
     6721 actgcgtcag gaagggttgc aaaaagctgt ttagccggtc cagcaacggc ggcacttgct
     6781 cgcgcagcag tggcaattgg ttgaccagaa tgggcaccac cagcagcagc agcgctagca
     6841 gcagcagcaa aaacacgatt tcaaccagcg ccaccgccaa cacgcgcggc agacggttgc
     6901 cgccgagccg gttgagccaa tcgaccaccg gggtcagcgc ataagccagg atggcggcca
     6961 ccacaaaggg tgtcagcacc ggtgccagca gccacagcgc caccaccaaa gccagcagca
     7021 atgcgatcca ggcgccaact cgtttttggg tgaaagtaaa ttgcattgca atggccaaaa
     7081 gccaggtcca tcaaacaaag ttccagccgc gaccagctag tcagcttgtg ccacaactaa
     7141 aatcaggtca aattctatcg ggcttgtcgc tttgcaacga tccgaccggc acctggtccc
     7201 accccatcac cgtttgaccc atcccatgac atcttctccc accccacccg tcgcactgtc
     7261 ctacaaagac tctggcgttg acattgatgc tggcgatgcg ttggtggagc gcatcaagcc
     7321 gtttgcaaga aagaccatgc gcgaaggcgt gctggcaggc attggcggct ttggcgcact
     7381 gttcgaactg cccaaacgtt accaggaacc agtgctggtg agcggtaccg acggggttgg
     7441 caccaagctc aagctggcat ttgactggca aatgcacgac accgtcggca ttgacctggt
     7501 cgccatgagc gtcaatgacg tgctggtgca aggggccaag ccgctgtttt tccttgacta
     7561 ctttgcttgc ggcaagctcg atgtcgatac cgccgccgcc gtcatcgcag gcattgcgcg
     7621 cggttgcgag ttgtcgggct gtgcactgat tggtggtgaa accgccgaga tgcccggcat
     7681 gtacccggct ggggaatacg acctggcagg ttttgccgtc ggtgcggtgg aaaagtccaa
     7741 aattctgacc ggggccgacg tcaggccggg tgacgtggtg ctgggcctgg caagcagcgg
     7801 tgtgcattcc aacggtttta gcctggtgcg gaaatgcatt gagcgggccc aggccactga
     7861 cacgattccc gatactttgg acggcaaacc gtttcgccat gccgtgatgg aaccaacccg
     7921 cctgtatgtc aagaatttgc tggccgcgct ggcagcccac ccgatcaagg cgctggccca
     7981 catcaccggc ggtggcctgc tggaaaacat tccgcgcgtg ctgccagaag gcacctcagc
     8041 gcacctgaaa aagggcagtt ggccacagac cgagttgttt gcctggctgc aaaagaccgc
     8101 cggcattgac gacttcgaga tgaatcgcac cttcaacaat ggcatcggca tggtgctggt
     8161 gattgatgcg accgaggccg cctcctgcgc cgccaccttg cgctctgcag gtgaagttgt
     8221 ttatgaaatc ggggtgattg ctgcgagtga tgaggccgcc agggtgctgg tgcggtagtc
     8281 tggcggcaag ctttggggtt gtttccgccc cgcttgatgc tttgtattta atagctgcca
     8341 acgcatattc cataagggct gcaagtagtt ttcgcttaaa aaaatgcgat gggccaaggc
     8401 aacgctaccc ggcgcaaaac tccgggccgc atctggatgc cgggttattt cagcctggtt
     8461 ttcaacagat tgctggtgct tttggctttt tggctttttg gctttttggc tttttggctt
     8521 tttggctttt tggcccaggc ggccatgaca ggttctgcgt caaggcactt aggatgtggt
     8581 cagaaataac ttcccctatt tgcactggcg gatttgggcg cacagcgtcg tt
//
LOCUS       DAOSLL010000132         8615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7665_length_8615_cov_4.475701, whole genome shotgun sequence.
ACCESSION   DAOSLL010000132 DAOSLL010000000
VERSION     DAOSLL010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8615
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7665_length_8615_cov_4.475701"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /locus_tag="PLB25_07680"
     CDS             complement(<1..1376)
                     /locus_tag="PLB25_07680"
                     /inference="COORDINATES: protein motif:HMM:NF013179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE41493.1"
                     /translation="MRAFKLTRYFSLLSLILLLLAGTLLGTLVRQHEITQMERVAEDR
                     NVNMTQMLGNLLRLDMDHLISRSLGQTRAELQAQPDIRPLHDKLARLIQGSDIAKVKI
                     YSLTGITVFSTEAAQIGEDKSGNAGFIAARNGQIASELVHRNHFSATERVLEDVDLLS
                     SYVPVLDGGQVVAVFEQYQDVTKLMHHIEQSLWQMGMIIFAVLSALYLMLLAVVRHAQ
                     TALTAQEAMLEAANRDLDQRVEERTHALRQSETRLRESEARFRSLTEMSSDFYWQSDV
                     NHRFTSRTLSAREAADPVFCQTSFVGLLRWQLPHVTPDEAAWQAHRALLAAHLPFRDF
                     EMSHQGAENKLLFVSVSGDPVFDARGTFTGYRGVGADITARKQVEIELSIAATAFDSQ
                     QSMLVTDANNVIQRVNTAFIESSGYSLQEIVGQSPRILQSGQHDRAFYTAMWDSIIQH
                     GHWHGELW"
     gene            complement(2004..2408)
                     /locus_tag="PLB25_07685"
     CDS             complement(2004..2408)
                     /locus_tag="PLB25_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOE41494.1"
                     /translation="MNKYMLGTDTCIFITRKSKPALLARIASVPLAQQCISVVTPAEM
                     LYGVQLSANQIANQQAVDLFVQHLEVLHWTPEAAKHYAEIRVDLKRLGHQIGSNDLMI
                     AAHARSLGAVIVTNNVTDFGRVKGLEFEKWME"
     gene            complement(2405..2644)
                     /gene="vapB"
                     /locus_tag="PLB25_07690"
     CDS             complement(2405..2644)
                     /gene="vapB"
                     /locus_tag="PLB25_07690"
                     /inference="COORDINATES: protein motif:HMM:NF040493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HOE41495.1"
                     /translation="MTLQTARIGKNNRVQVVTIPLEFRFPPGMKDVYIRKVGNDLILS
                     PRPANWQSFLQSELCATADFMQGVDDLPVQERSFT"
     gene            2959..4344
                     /gene="yjjJ"
                     /locus_tag="PLB25_07695"
     CDS             2959..4344
                     /gene="yjjJ"
                     /locus_tag="PLB25_07695"
                     /inference="COORDINATES: protein motif:HMM:NF007297.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family toxin
                     YjjJ"
                     /protein_id="HOE41496.1"
                     /translation="MAPRTLADPRLHLPRLLLKNPGAKSTELAGLAQVSTPTMVRMLN
                     EMEVDVLRLGKARGTRYFLRRSLRGLPSSAPVFALDQDGGASKVGQLALIAPQGTVLD
                     VAGMGWPVEKEFAHGVWPDGLPYPLQDMKPQGFMGRQFAHQESSALGISSNPGEWCDD
                     DILLILLSRGADTSGNLIVGDTALSLWLQSKAGPQTVLGEDSVLAGYLACAEQASSRG
                     IAGSSAAGEFPKFTALRELPGARTVHVIVKYSANDRSDTVRRWSDLLICEHHALQAIR
                     RIPGVQSASSRVLQHGGRTFLEVERFDRHGLFGRSPLCSLDTLEASMLPGTSTDWGDA
                     GDMMRAQGWLNADAAKHLRSIWAFGKLIGNTDMHKGNLSFVPGPNMQVAPVYDMLPMA
                     YAPMAGGEIPTPGYAPGLPKPKDRDAWLAASQAAQDFWLRAMQDDRISERFRQISEAN
                     LQELDRLMKLA"
     gene            complement(4411..5049)
                     /locus_tag="PLB25_07700"
     CDS             complement(4411..5049)
                     /locus_tag="PLB25_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HOE41497.1"
                     /translation="MAWTHDQMAAIAAKELQDGFYVNLGIGLPTLVANYVPAEVEVWL
                     QSENGMLGIGPFPTEDEVDADLINAGKQTVTTIAGSSIFGSHDSFAMIRGGKINLSIL
                     GAMQVSERGDLANWMIPGKMVKGMGGAMDLVGGVRSVVVLMEHVAKQKDGSIDLKILP
                     HCTLPLTGVGVVDRIITDLAVMDITPHGIKVVQLAPGVTMQDLQEVTGVSLH"
     gene            complement(5052..5759)
                     /locus_tag="PLB25_07705"
     CDS             complement(5052..5759)
                     /locus_tag="PLB25_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HOE41498.1"
                     /translation="MNKIYPSAAAALKGVARNGQLLAVGGFGLCGIPEALIDALRDSG
                     VKDLTVVSNNAGVDGFGLGKLLETRQIKKMISSYVGENKEFERQYLAGELELEFTPQG
                     TLAEKLRAGGAGIPAFYVRTGVGTLVAEGKENRVFDGHVYILEHALVPDLSLVKAWKA
                     DKSGNLLFRRTARNFNPAVAMAGKVTVVEVEEIVDIGTFDPDEVHLPGIYVNRIVLNA
                     TPEKRIEKRTITHKAGV"
     gene            5923..6057
                     /locus_tag="PLB25_07710"
                     /pseudo
     CDS             5923..6057
                     /locus_tag="PLB25_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
     gene            6170..6766
                     /locus_tag="PLB25_07715"
     CDS             6170..6766
                     /locus_tag="PLB25_07715"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HOE41499.1"
                     /translation="MVTTTKAPKTKAATDKAAPKTTDTAKTVAAKAPAAKAPAVKAPA
                     VKTPVAAKKAAAPAVPVVAAPPAAPVVAKAPAPVVAAAPAPVVAAAPAPAPAPAPAPV
                     VAAAPAPVAALQTSPALTPMPARKPATKKVSDSSNPEAPATAARPALTTEQRNKYVAV
                     AAYHIAELRGFTSGDPRDDWLAAEAEVDRLIASGHVFE"
     gene            complement(6768..7187)
                     /locus_tag="PLB25_07720"
     CDS             complement(6768..7187)
                     /locus_tag="PLB25_07720"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOE41500.1"
                     /translation="MKFDIPEDKKLVFEVVIPIRWGDMDAMGHVNNTTYFRYMETIRI
                     DWLRAIGGMPNPLGEGPVIVNAFCNFYKQLEYPGDVLVKMYTSDPARTTFETWATLET
                     PDQPDVIYAAGGATTIWVDFPKQKAIELPQWMRSIVS"
     gene            complement(7221..7778)
                     /locus_tag="PLB25_07725"
     CDS             complement(7221..7778)
                     /locus_tag="PLB25_07725"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: protein motif:HMM:NF016248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HOE41501.1"
                     /translation="MTRWVSRNPIRGKLHRWLQASGSLSARLAATGERFTVQVLNQGR
                     QSLTVDEAQALGTGARAGYAREVLLRVDGVPLVFARSVTRHVDSVGAWRSLRGLGSRP
                     LADVLFNRAGMVRSPLTFARLAQTSRLQRHVQACWQAATGQHKVCAAFPARRSVFVWH
                     GAALLVMEVFVADATNWSRQPKKTR"
     gene            complement(7775..8614)
                     /locus_tag="PLB25_07730"
     CDS             complement(7775..8614)
                     /locus_tag="PLB25_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOE41502.1"
                     /translation="MPRLTPGLSPVDLTAGRFVSFGAIAALVMLLTWRSHTWPTWRQA
                     ASALGLSLLGATGYYLLLAQAINAAGSEVPTLIIGTIPIWVMLLGKPLGLKWSALVPG
                     LLLTAAGLALMMHASLSNATQLPLDGENFWWGVLLAVAAMLSWTAFALLNAAWLKRHP
                     EISATEWANWLGVATGLGALVLWLLTGSESKVLLSLDGIALIAIVCIVTGIGAGWAAS
                     ILWNLASQKLSVSLCGQLIVSETLFALLCSFAWDGNWPTVAQWWAALLFTLGILASIQ
                     AHR"
BASE COUNT         1849 a         2614 c         2433 g         1719 t
ORIGIN      
        1 ctccagagtt caccatgcca gtgcccgtgc tggatgatgc tgtcccacat ggcggtataa
       61 aaagcccggt catgctggcc cgactgcaaa atgcgtggcg actggccgac aatttcctgc
      121 aggctgtagc cggaagattc aataaaggcg gtattgacgc gttgaatcac attattggca
      181 tcggtgacca gcatgctttg ctgtgaatcg aaggcggtcg ccgcgatgct gagctcaatt
      241 tccacctgct tgcgcgcggt gatgtcggca ccaacgcccc ggtagccggt aaatgtgcct
      301 ctagcatcaa acaccgggtc gccgctgacg ctgacaaaca gcagcttgtt ttccgcgccc
      361 tggtgcgaca tttcaaaatc acgaaacggc agatgggccg ccagcagcgc ccgatgtgcc
      421 tgccaagcgg cctcatcggg cgtgacatga ggcagttgcc agcgcaaaag accgacgaac
      481 gatgtctggc aaaagaccgg atcggccgcc tcccgggcgc tcagggtgcg tgaggtaaac
      541 cggtggttta cgtcactttg ccaataaaag tcagatgaca tttccgtcag gctgcgaaac
      601 cgtgcctcgc tctcgcgcaa acgggtttcg ctttgccgca gggcatgggt tcgctcttcc
      661 acacgctggt caagatcacg gttggcggct tccagcatgg cctcttgcgc cgtcagcgcg
      721 gtctgcgcat gcctcaccac cgccagcagc atcaggtaca gcgcgctgag caccgcgaag
      781 atgatcatcc ccatctgcca caaagactgt tcaatgtggt gcatcagctt ggtcacgtcc
      841 tggtactgct caaacaccgc caccacctga ccaccgtcca agaccggaac atagcttgac
      901 aacaaatcca cgtcctccag cacccgctca gtcgcactga aatggttgcg gtgcaccagt
      961 tcgctggcaa tctggccatt gcgcgccgcg ataaacccgg cattgccgct tttgtcttcg
     1021 ccaatctgcg cggcttcagt cgaaaaaacc gtgatgccgg tcaagctgta gattttgacc
     1081 ttggcaatgt ccgagccctg aatcagccgg gccagcttgt catgcaaagg ccggatatcc
     1141 ggttgggctt gcagctcggc acgggtttgg ccaagcgagc gggagatcag gtggtccatg
     1201 tccaggcgca gcaagttgcc cagcatctgg gtcatgttca cattgcggtc ttcggccacc
     1261 cgctccatct gggtgatttc atgttgccgg accaaagtgc ccagcagcgt accggccagc
     1321 aacagcaaga tcagcgacaa cagcgaaaaa tagcgggtta gtttgaaggc tcgcattcat
     1381 tgacccactt tcaaatctgc catctggccc catgccagtg atcttgaacc attcaggttt
     1441 gcgtcatttc cgtcggttta taacacctgc ggggcaactg ccacgcgcag gggctttgcg
     1501 ggcattgcca cacgccatga cggcgccgac tgccgggcag ccacccatct ggcaaggatg
     1561 tcagtcacaa gatggcagtt tgcgccgctg cagcgggcct cagtcgagca gcagcatcag
     1621 gatgggcatc aggtcaaaca tctggacggt ggcactcgct tggtcggcta acgcaaactg
     1681 gatagcaagg tcgcttgtgt caaacgctgc aggttgccgg aaaggggtct atgagaccgg
     1741 atcaaaagct agccaaggca gcaacgccgt tgcgtttgcc atgcgaatga tgacggacag
     1801 agggtccatg gcgttgccat cggtgaacat acagccttgg ccagtctcat tgatcggctt
     1861 ggcttcaaac atttataaga aatcaggcgc tagcccttat cagataagcg caactagcta
     1921 ttattattga agtgatctag gtaccagaca acaagaccgc aaacacctgg catcaccagc
     1981 ccagccgcca ccagttctga gtcctactcc atccattttt cgaattccag ccctttgact
     2041 ctgccgaagt ctgtgacgtt gttggtcacg atcacggcac ccaggctgcg ggcgtgtgct
     2101 gcaatcatca ggtcgttgga gccgatttgg tggccgagcc ttttcaggtc cacacgaatt
     2161 tcggcatagt gtttggctgc ttcgggggtc cagtgaagaa cttcaaggtg ttgtacaaac
     2221 aaatcgaccg cttgctggtt agcaatctgg tttgcagaca actgaacgcc atacagcatt
     2281 tccgccgggg tgacaacaga aatgcactgc tgcgccagag gcacggacgc aatgcgcgca
     2341 agcaaagcgg gctttgattt gcgcgtgatg aagatgcaag tgtctgtgcc cagcatgtac
     2401 ttgttcatgt gaacgaccgc tcttgcaccg gcaaatcgtc aaccccttgc ataaagtcag
     2461 cggtcgcgca caactccgac tgcaagaacg actgccagtt cgcagggcgc ggagaaagaa
     2521 tcagatcgtt gcccaccttg cggatgtaaa cgtccttcat gcctggcgga aatctgaact
     2581 ccaagggaat cgtcaccact tggacgcgat tgttcttgcc gatgcgggcg gtttgtaagg
     2641 tcacaatgta cttctgtttg ccatgtatct aattacatcg cagtgatcga cattggtcag
     2701 aatgtcaatg cgcccaagcg agaccgcagg ttggtgcctg caatgacgca ccatgctctg
     2761 agcttgccgg aatcactgtt gtgcactgcc agttcagcac tctccaaatg ccgaagtgcc
     2821 ccttttttag gatgcaaata ttgaatcacg aaaatcgcag aatcgatgaa ggagggagcc
     2881 atcccattgg taaactgtgg caagaaatgc gatttattgc agtttattga atcaaagatc
     2941 aaatcaaatc aggtgaccat ggcaccaaga acactggctg accccaggct tcacttaccc
     3001 cgtttgctgc tgaagaaccc cggtgccaaa agcactgaac tcgccggcct ggcgcaggtc
     3061 agtaccccga ccatggtgcg gatgcttaac gaaatggagg ttgatgtact gcgtctgggc
     3121 aaggcccggg gaacacgtta ctttctgcgc cgcagtctgc gcggtttgcc ttcctcggca
     3181 ccggtattcg cgcttgatca ggatggcggt gcgtcaaagg ttggtcaact ggcgttgatt
     3241 gcgccccaag gaacagttct cgacgtagcg ggcatgggat ggcccgttga gaaagaattt
     3301 gcccatggtg tttggccgga tgggctgcct taccccctgc aggacatgaa gccgcagggc
     3361 ttcatggggc ggcaatttgc ccaccaggaa tccagcgcac ttggcatctc gtccaatcca
     3421 ggggaatggt gtgatgacga tatcctgctg atcttgctaa gccggggagc agacaccagc
     3481 ggcaacctga ttgtgggcga taccgctctc agtttgtggc tgcagagcaa agccgggccg
     3541 cagaccgtgc tcggtgaaga cagcgttctt gcgggttatt tggcttgcgc agaacaggcc
     3601 agttcaaggg gcatcgcggg ctccagcgcc gccggtgagt tcccgaaatt caccgccttg
     3661 cgtgaactgc caggcgcccg caccgtgcac gtcattgtga agtactcggc caatgatcgc
     3721 tctgacaccg tccgaaggtg gtctgacctg ctgatctgcg agcaccacgc gctacaggcg
     3781 atacggcgca ttccgggtgt tcaaagtgcc agcagcaggg tgctgcaaca cggcgggcgc
     3841 acttttctgg aggttgaacg cttcgaccgg catggcttgt ttggccgcag tcccctgtgc
     3901 agtcttgaca ccctggaggc atcgatgttg ccaggcacca gcacagactg gggtgatgcc
     3961 ggcgacatga tgcgggcaca gggttggcta aatgcagacg ctgccaaaca tctgcgcagc
     4021 atctgggcct ttggcaaact catcggcaac acagacatgc acaaaggcaa cctcagcttt
     4081 gttcccggac ccaacatgca ggtagcgccg gtgtacgaca tgcttcccat ggcctacgca
     4141 ccgatggctg ggggagaaat tccaacaccc ggttacgcgc cggggttgcc aaagccaaag
     4201 gaccgggacg catggctggc ggcaagtcag gcggcgcagg atttttggct tcgggcgatg
     4261 caagatgatc gaatcagtga gcggttcagg cagatcagcg aggcgaacct gcaggaactc
     4321 gatcgcctga tgaagctggc ctgagccaaa gcaagccagc aaaaatcaag gccaacccgg
     4381 tgcagtcctc aatgtagcct caatgcagcc tcaatgcaga gatacgccgg tgacttcctg
     4441 caggtcttgc atcgtcacgc cgggtgccag ttgcaccacc ttgatgccgt gcggcgtgat
     4501 gtccatcacc gccaggtcgg taatgatgcg atccaccaca ccgacaccgg tcagcggcaa
     4561 ggtgcagtgg ggcaagatct tcaggtcaat gctgccgtct ttctgtttcg ccacatgctc
     4621 catcagcacc accacgctgc gcacgccgcc gaccaggtcc atcgcgccgc ccatgccctt
     4681 gaccatcttg cccggaatca tccagttggc caggtcgcct cgttcgctga cctgcatggc
     4741 ccccagaatg ctcaggttga tcttgccgcc gcgaatcatg gcaaagctgt catggctgcc
     4801 aaaaatgctg gaaccggcaa tggtggtgac ggtttgcttg ccggcgttga tcaggtcggc
     4861 atcgacctcg tcttcggtcg gaaacgggcc aatgccgagc atgccgtttt cgctttgcag
     4921 ccagacctcc acctctgcgg gcacataatt ggccaccagc gtgggcaggc caatgccgag
     4981 gttgacgtaa aaaccgtcct gcaattcctt ggccgcaatg gccgccattt ggtcgtgggt
     5041 ccaggccatg atcagactcc tgctttgtgg gtgatggttc tcttctcaat gcgcttttcg
     5101 ggcgtggcat tgagcacgat gcggttcacg taaatgccgg gcaaatgcac ctcatcgggg
     5161 tcaaacgtgc cgatgtcaac aatttcctcg acttccacca cggtgacctt gccggccatg
     5221 gccactgccg gattgaagtt gcgggcggtg cgccgaaaca gcaagttgcc gctcttgtcg
     5281 gccttccagg ccttgaccag cgacaggtcg ggcaccagcg cgtgctccag aatgtaaaca
     5341 tggccgtcga acacgcggtt ttccttgcct tctgccacca gtgtgccgac cccggtgcgc
     5401 acataaaacg ccggaatacc ggcaccgcca gcgcgcagct tttcggccag cgtgccttgc
     5461 ggggtgaatt caagttcgag ttcaccggcg aggtattggc gctcaaattc cttgttctcg
     5521 ccgacgtagc tggaaatcat ttttttgatc tggcgcgttt ccagcagttt gcccaggcca
     5581 aagccatcga caccagcatt gttggagacc accgtcaggt ctttcacacc cgaatcgcgc
     5641 aatgcatcaa tcaaggcctc gggaatgccg cacagtccaa agccgcccac tgccagcaac
     5701 tggccgtttc tggcgacacc tttgagcgca gcggctgcag aagggtaaat cttgttcact
     5761 caattttctc cgggtagtaa tcactggcgt gaggatacta cctggctccg tgcgtcgttc
     5821 ttcttgaggc ccaagccaga tgcgctgccg ttggcccggc cccggctgcc aaagcttgcc
     5881 agggccattg aacaccgccc ggcgttgatg ctggccccgg tcggcctgac atccagggaa
     5941 atcggccggg ctctgacgat cagctaccgc acggttgcca tttaccgcgc ccgcctgacg
     6001 cgtatgtaca acgtagccaa taccggcgag ttggatcaca aattaatggc gggataatgc
     6061 gccctgtatg gcacgtgttt tgcgtgattt aatgtatgcc gcgtgccagg ctcgtgcgac
     6121 gcaagtcgcc ccaaaaaatc aatcttgttc ttaacctgaa ggaaacgcaa tggtcactac
     6181 taccaaagcc cccaaaacca aggcggcaac tgacaaagca gcgcctaaga cgaccgatac
     6241 cgcgaaaact gttgcagcca aagcaccggc tgccaaagcc ccagccgtca aagccccagc
     6301 cgtcaagaca ccggtcgcag ccaaaaaagc cgctgcacca gcggtgccgg ttgttgccgc
     6361 gcccccggct gctcccgttg tagccaaagc cccagccccg gtggttgctg cggcgcctgc
     6421 gccggttgtc gccgcagcac ctgcacctgc acctgcacct gcacctgcac cggttgtcgc
     6481 cgcagcacct gcaccggttg cagcattgca gaccagcccg gcactaaccc cgatgccagc
     6541 gaggaagccg gctaccaaga aggtctctga cagttcaaac ccggaggcac ctgcaaccgc
     6601 tgccaggccc gcgctgacca ccgagcagcg caacaaatac gttgccgttg cggcctatca
     6661 cattgccgag cttcgcggtt ttacctcagg tgatccgcgc gacgactggt tggcagccga
     6721 agccgaggta gaccgtctga ttgccagcgg tcacgttttc gaatagctca actgacaata
     6781 ctgcgcatcc actgcggcag ttcaatcgcc ttttgcttcg gaaaatccac ccagatggtg
     6841 gtggccccgc ctgccgcata gatcacgtcg ggctggtcgg gggtttccag cgtggcccag
     6901 gtctcgaacg tggtgcgtgc cgggtcgctg gtgtacatct tgaccagcac gtcgcccggg
     6961 tattcaagtt gcttgtaaaa gttgcaaaag gcattgacga tcaccgggcc ctctcccagc
     7021 gggttgggca tgccgccaat ggcgcgcaac cagtcgatgc ggatggtttc catgtacctg
     7081 aaataggtcg tgttgttgac gtgccccatg gcatccatgt cgccccagcg gatcgggatg
     7141 accacttcaa acaccagttt cttgtcttcg ggtatgtcga atttcatgtt ttttcctcag
     7201 tagcaaacaa ctgcgggttg ttatctggtt tttttcggct gcctcgacca atttgtcgca
     7261 tcggcaacga atacctccat caccagcagc gctgcgccat gccatacaaa caccgagcgg
     7321 cgcgccggaa aggccgcgca caccttgtgc tgaccggttg ccgcctgcca gcaggcctgg
     7381 acatgacgct gcaagcggct ggtctgcgcc agccgggcaa acgtcaacgg gctgcgcacc
     7441 atgccagcac gattgaacaa cacatcggcc agcggtcgcg agcctagtcc gcgcagcgag
     7501 cgccaggcac ccactgaatc gacatgccgg gtaacgctgc gggcaaatac caagggcacg
     7561 ccatccaccc gcaacagcac ttcgcgggca tagccggcgc gagcgccagt gccaagtgcc
     7621 tgtgcctcat cgaccgtcag gctctggcgg ccctggttca acacctgaac ggtaaagcgc
     7681 tcgccggtgg cggccagacg cgcactcagc gagccggatg cctgtagcca gcggtgcagc
     7741 ttgccgcgta tcggattgcg tgaaacccag cgtgtcatcg gtgcgcctga atcgatgcca
     7801 gtatgcccaa agtaaacagc agcgcggccc accactgcgc cacggttggc cagttgccgt
     7861 cccaggcgaa tgaacaaagc agagcgaaca aggtctcgct gacgatcagt tgaccgcaca
     7921 gactgacact caatttctgg ctggccagat tccacaagat gctggccgcc caacccgcac
     7981 cgatcccggt tactatacaa acaatagcta tcagcgcaat accatcaagg gatagaagca
     8041 cttttgactc tgaacccgtg agcagccaaa gaaccaatgc gcccaggccg gtagccacac
     8101 caagccagtt cgcccattcg gtcgcactga tttcggggtg gcgcttgagc caggccgcgt
     8161 tcagcagtgc aaacgccgtc cagctcagca tggccgccac cgccagcagc acaccccacc
     8221 agaagttttc gccgtcaagt ggcaattggg tcgcgttgga cagcgacgcg tgcatcatca
     8281 gcgccagccc ggcggccgtc aacagcaggc caggaaccag cgccgaccac ttcaagccca
     8341 gcggtttgcc cagcaacatg acccagatcg gaatggtgcc gatgatcagc gtgggtactt
     8401 ccgagcccgc tgcgttgatg gcttgcgcca gcagcaaata gtagccggtt gcacccagca
     8461 ggctcaaacc cagggcgctt gccgcctgcc gccaggttgg ccaggtatgg ctgcgccagg
     8521 tcagcagcat gaccaacgcc gcgatggccc cgaaactgac gaaacgcccg gccgtcaagt
     8581 caaccgggga caggcccggt gtcaggcgtg gcacc
//
LOCUS       DAOSLL010000133         8512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7777_length_8512_cov_4.510583, whole genome shotgun sequence.
ACCESSION   DAOSLL010000133 DAOSLL010000000
VERSION     DAOSLL010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8512
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7777_length_8512_cov_4.510583"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            493..1680
                     /locus_tag="PLB25_07735"
     CDS             493..1680
                     /locus_tag="PLB25_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOE41503.1"
                     /translation="MQFADRLQNVETSAIRELFKLLGKPGIISFAGGFPDPALFDAEG
                     IAMASQAVLANNPGPVLQYGATEGYQPLREKISDFMAAKGCSVSPDGLIVTTGSQQAL
                     DLIGKTMISPGDKVIVEAPTFLATIQCFRLYGADIIGAPIDADGVDVGQLEKLIEEHQ
                     PKLIYLIPTFGNPSGATLSLQRRKQILEIAVRTQTLVVEDDPYGELFFDQPPPPSLLS
                     LSDDVPGSRDWLAHCGSFSKILSPGLRVGWMIAPAELLAKATMCKQFSDAHTSNLTQA
                     ICAHYLTLNRLGDALAVVRTTYAGRAQVMARCLKRELGDAVDFNAPQGGMFLWARLTG
                     SNGRTNIAADFAKRAIDHGVAFVPGAPFFAHQPDMSTFRLSFATADVARIEEGIERLG
                     RAL"
     gene            complement(1967..2230)
                     /locus_tag="PLB25_07740"
     CDS             complement(1967..2230)
                     /locus_tag="PLB25_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41504.1"
                     /translation="MVRWLLFGLVVSVFQPVVDAPGKLWQHKLLQALIGLLFGAVCAV
                     VFTPAENRFNTPRVSWKSWLLVVMTWLLVKVAFVSMLAIADAQ"
     gene            2471..2608
                     /locus_tag="PLB25_07745"
     CDS             2471..2608
                     /locus_tag="PLB25_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41505.1"
                     /translation="MTSMQPAVAALLGRLMLDEHLNALLRLTIVCIMLAATGSLVTVR
                     S"
     gene            complement(2686..3147)
                     /locus_tag="PLB25_07750"
     CDS             complement(2686..3147)
                     /locus_tag="PLB25_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOE41506.1"
                     /translation="MDLVLWRHAEAHEWTQGCDDMARPLTARGERQAARMATWLDRQL
                     PENTRIFASPACRAEQTASALDRKFKVKPTLGPMCVASDLLELVQWPDAKGTLLVVGH
                     QPTLGQVIAQLIGLKLAECPVRKGSVWWLRNRQRETFSQTVVVAVQSPELL"
     gene            complement(3814..4260)
                     /locus_tag="PLB25_07755"
                     /pseudo
     CDS             complement(3814..4260)
                     /locus_tag="PLB25_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            4253..4699
                     /locus_tag="PLB25_07760"
                     /pseudo
     CDS             4253..4699
                     /locus_tag="PLB25_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463245.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            5366..5827
                     /locus_tag="PLB25_07765"
     CDS             5366..5827
                     /locus_tag="PLB25_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOE41507.1"
                     /translation="MDLVLWRHAEAHEWTQGCDDMARPLTARGERQAARMATWLDRQL
                     PENTRIFASPACRAEQTASALDRKFKVKPTLGPMCVASDLLELVQWPDAKGTLLVVGH
                     QPTLGQVIAQLIGLKLAECPVRKGSVWWLRNRQRETFSQTVVVAVQSPELL"
     gene            complement(5905..6042)
                     /locus_tag="PLB25_07770"
     CDS             complement(5905..6042)
                     /locus_tag="PLB25_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41508.1"
                     /translation="MTSMQPAVAALLGRLMLDEHLNALLRLTIVCIMLAATGSLVTVR
                     S"
     gene            6283..6546
                     /locus_tag="PLB25_07775"
     CDS             6283..6546
                     /locus_tag="PLB25_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41509.1"
                     /translation="MVRWLLFGLVVSVFQPVVDAPGKLWQHKLLQALIGLLFGAVCAV
                     VFTPAENRFNTPRVSWKSWLLVVMTWLLVKVAFVSMLAIADAQ"
     gene            complement(6833..8020)
                     /locus_tag="PLB25_07780"
     CDS             complement(6833..8020)
                     /locus_tag="PLB25_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOE41510.1"
                     /translation="MQFADRLQNVETSAIRELFKLLGKPGIISFAGGFPDPALFDAEG
                     IAMASQAVLANNPGPVLQYGATEGYQPLREKISDFMAAKGCSVSPDGLIVTTGSQQAL
                     DLIGKTMISPGDKVIVEAPTFLATIQCFRLYGADIIGAPIDADGVDVGQLEKLIEEHQ
                     PKLIYLIPTFGNPSGATLSLQRRKQILEIAVRTQTLVVEDDPYGELFFDQPPPPSLLS
                     LSDDVPGSRDWLAHCGSFSKILSPGLRVGWMIAPAELLAKATMCKQFSDAHTSNLTQA
                     ICAHYLTLNRLGDALAVVRTTYAGRAQVMARCLKRELGDAVDFNAPQGGMFLWARLTG
                     SNGRTNIAADFAKRAIDHGVAFVPGAPFFAHQPDMSTFRLSFATADVARIEEGIERLG
                     RAL"
BASE COUNT         1860 a         2396 c         2396 g         1860 t
ORIGIN      
        1 gacagaacgc ggaaccaaaa ttaacgtggg tttcattggg gaaactcggc atttttttgg
       61 aaaagaactt cggcttaaaa ctcaaacagg acaaaaaaca tggatccgct ttggaggatc
      121 acccggcaag cattcgaccc ccccatcccg tctactgaag caacatggca gcgaagtttt
      181 tgcatagcca aaggagccag atatccttga tggtactaag cgccatgcca ctgacgggct
      241 tcttgccgcg cccactggta ccaccagcat tggtccaaat cgcggccttg tggcaagtgc
      301 ctttgtggcg ttcagattcg acaactgact tgccttcaac cgtgtaccaa cagtcaaaga
      361 tgcaccggca gtcttggcgc ggccaggaaa ggggcagtct tgataacatc acccacattc
      421 tgaaaactct ctattgatgc gacctggcct cttttttggg tttttaacat tccaaccgtt
      481 gagtcacaca ccatgcaatt cgccgaccgc ctccaaaacg tggagacctc tgccatccgt
      541 gaacttttca agctgcttgg caaacctggc atcatcagct ttgctggcgg ctttccggat
      601 ccggcgctgt ttgatgcaga aggcattgcc atggcttcac aggccgtgct ggccaacaac
      661 cctggcccgg tactgcaata tggtgcgact gaaggctatc aaccgctgcg cgaaaaaatc
      721 tccgacttca tggcagccaa gggttgcagc gtctcacctg acggcctgat tgtgaccacc
      781 ggcagccagc aggcgcttga cctgattggc aaaaccatga tctcgcccgg cgataaggtg
      841 atcgttgaag cgccgacatt tctggccacc attcagtgct ttcggctgta tggtgcagac
      901 atcatcggtg caccgattga tgcagatggc gtggatgtgg gccagctcga aaaactcatt
      961 gaagagcatc aacccaaact gatttacctg atccccacgt ttggcaaccc cagcggcgcc
     1021 acactcagcc tgcagcggcg caagcagatt ctggaaattg ccgtgcgcac gcaaaccctg
     1081 gtggtggaag atgacccgta cggcgagctc tttttcgatc aaccgccgcc gccgagcctg
     1141 ctgagcctga gcgatgatgt gcccggcagt cgtgactggc tggcgcattg cggttcgttc
     1201 agcaagatac tgagcccggg attgcgggtt ggctggatga tcgcaccggc ggaactgttg
     1261 gccaaagcca ccatgtgcaa gcagttcagc gacgcgcaca ccagcaacct gacacaagcc
     1321 atttgcgccc attacctgac gctgaaccgc ttgggcgatg cgctggcggt ggtgcggaca
     1381 acttacgctg ggcgtgccca ggtcatggcg cgttgcttga aacgcgagtt gggggatgcc
     1441 gttgatttca acgcgcccca gggtgggatg ttcctctggg ctcgcctgac ggggtccaac
     1501 ggacgcacca acatcgccgc cgattttgcc aagcgcgcca ttgatcatgg cgttgccttt
     1561 gtgccgggcg cgcccttttt tgcgcatcag ccagatatgt caactttccg cctgagtttt
     1621 gccaccgccg atgtggcaag aatcgaagaa ggcattgagc gcttgggtcg ggcgctctga
     1681 gtccgtttgg gtacttcgaa ctgaaaccac ctccaacaac atcaattttt taagcaccac
     1741 cgcaaaatag accatcgcaa caggcacagc gcacgggcgt tgaccggtct gaagaagtca
     1801 atccattgca tcattgatct gcctgagggg gacttcaacc gcagccgaac tggctttggc
     1861 gatagcgtga aataactttc atgtccgggg gtgaatggag tccatgaaag ttaacgtgat
     1921 ggtcaatgga acgtcagagc ttggtcctgg gtgatgctca gcagtgttac tgcgcatcgg
     1981 caattgccag catgctgaca aaggcaactt tgaccagcag ccaggtcatc accaccagta
     2041 gccaagactt ccaactgacc cgtggcgtgt tgaagcggtt ttcagcaggc gtaaaaacga
     2101 ccgcgcagac cgccccgaaa agcagcccaa tcagtgcttg gagtagtttg tgctgccaga
     2161 gcttgccggg ggcatcaacg accggctgga agacactgac caccaagccg aacaacaacc
     2221 atctcaccat gtagccgcgc caccgatacc aggggccagg ttcagatttt gaccaatttg
     2281 ccgggtttgg cgttgaggac tgcatgcgtc gtgttcacaa aagttgccat gccattttaa
     2341 atgtcaggca cgcccggaaa aatccagaag gggcagtaaa ccaggtttcc agccaggtat
     2401 ctgctcaatc gacaaggaaa cgtggtccac acagtgccca agaaagaagt caactgaagg
     2461 gtccgggatc atgaccagta tgcagccagc agtggcagca ctgctgggca ggttgatgct
     2521 cgatgagcac ctcaacgcct tgctgcggtt gaccatcgtt tgcatcatgc tggccgctac
     2581 cggcagtttg gttaccgtgc gcagctagcg aaaaaagcaa tgcgccacaa gagatcatct
     2641 tgcgaggtac cgttggctgt cggcttgcgg taaatcagcc gggaatcaaa gcaactctgg
     2701 cgattgcacg gcaaccacca cggtttgtga aaaagtctca cgctggcgat tgcgcaacca
     2761 ccaaactgag cccttcctga ctggacactc tgcaagcttc aatccaatca actgggcgat
     2821 gacttgcccc agagtcggtt ggtggcccac gacaagcagt gtcccttttg catctggcca
     2881 ttgcaccaat tccagcaagt cggaggcaac gcacatcggc ccaagtgtgg gtttgacttt
     2941 gaattttcga tcaagggcac tggcggtttg ttcggcgcga cacgccgggc tggcaaaaat
     3001 acgcgtgttt tcgggtaact gccggtcaag ccaggtggcc atgcgtgccg cctgcctttc
     3061 accgcgtgcg gtgagcggtc gcgccatatc gtcacagccc tgagtccatt cgtgcgcctc
     3121 agcgtgacgc cacaaaacca gatccatgcc agccgccttt tttgccttgt caccggtcgg
     3181 atgccactca catttgaatg gcaacgaaga tggcgttggt cgcaccacgt tctcgctcac
     3241 tgatgctgtt tcgtccggga tgatcgccct gacaccaaca tgtctggaat gtaacctgaa
     3301 aaagcggcag ttgcatgaca ttgccctact gataacagtg gtattgccag gccagcttct
     3361 tgcagtgtca atgacccgga agtggtggtt gcccgagtgc gcattttggt gatccgcagg
     3421 cagtgggaat cggcgctgcc ctcgacgttt cgcgatcacc cgtggtgctg ttgtcaggct
     3481 ggcaccggta tctaaagttc aatgtgatat tgggctgtag cccttgtgtc acatgcctga
     3541 catgctgcaa caaatatagt aactcagcac tgttcctcgt tcagatgcga tgcaatcgcc
     3601 gtggcctgct gagctgctga gctggtgatc tggtgatctg gtcagggtcg cctcaagctg
     3661 gcaacacaag gtgccggcca tgagcgagcg gctcaagccc ggctttggcc aacacaaggt
     3721 gcgccaaatc ctgcctgcgc gataccggat tgccccaacg ccatgatgca gacgcgatgg
     3781 tgtggcaagt gttcattgga tgacctgcgt gcatcaggca ccggctgcaa tccggagcat
     3841 cgactggggt gcaaccgggt tgccttgcag tttgaagatg gaaacgagtt gcaccaaatc
     3901 ctgcgcttgc gatttcaggc tgcctgctgc ggcggccatt tcttcaacca gcgcggcatt
     3961 ttgctgcgtc gcctggtcca tttgcgtgac tgcttcacca atctggttga cccccatgct
     4021 ctgttcgtta ctggcgctgc tgatttcgct catcaggtcg gtgacgcgcc ggatcgcgct
     4081 gaccacctcg gtcatggtga ctccggcttg atcaaccagg ctggtccctt gcgttacccg
     4141 atccacactg gcgctgatca gcaacttgat ttccttggct gcctcggctg agcgcccggc
     4201 taacgagcgc acctcgctgg caaccaccgc aaagccgcgt ccttgctcgc cagcacgtgc
     4261 tggcgagcaa ggacgcggct ttgcggtggt tgccagcgag gtgcgctcgt tagccgggcg
     4321 ctcagccgag gcagccaagg aaatcaagtt gctgatcagc gccagtgtgg atcgggtaac
     4381 gcaagggacc agcctggttg atcaagccgg agtcaccatg accgaggtgg tcagcgcgat
     4441 ccggcgcgtc accgacctga tgagcgaaat cagcagcgcc agtaacgaac agagcatggg
     4501 ggtcaaccag attggtgaag cagtcacgca aatggaccag gcgacgcagc aaaatgccgc
     4561 gctggttgaa gaaatggccg ccgcagcagg cagcctgaaa tcgcaagcgc aggatttggt
     4621 gcaactcgtt tccatcttca aactgcaagg caacccggtt gcaccccagt cgatgctccg
     4681 gattgcagcc ggtgcctgat gcacgcaggt catccaatga acacttgcca caccatcgcg
     4741 tctgcatcat ggcgttgggg caatccggta tcgcgcaggc aggatttggc gcaccttgtg
     4801 ttggccaaag ccgggcttga gccgctcgct catggccggc accttgtgtt gccagcttga
     4861 ggcgaccctg accagatcac cagatcacca gctcagcagc tcagcaggcc acggcgattg
     4921 catcgcatct gaacgaggaa cagtgctgag ttactatatt tgttgcagca tgtcaggcat
     4981 gtgacacaag ggctacagcc caatatcaca ttgaacttta gataccggtg ccagcctgac
     5041 aacagcacca cgggtgatcg cgaaacgtcg agggcagcgc cgattcccac tgcctgcgga
     5101 tcaccaaaat gcgcactcgg gcaaccacca cttccgggtc attgacactg caagaagctg
     5161 gcctggcaat accactgtta tcagtagggc aatgtcatgc aactgccgct ttttcaggtt
     5221 acattccaga catgttggtg tcagggcgat catcccggac gaaacagcat cagtgagcga
     5281 gaacgtggtg cgaccaacgc catcttcgtt gccattcaaa tgtgagtggc atccgaccgg
     5341 tgacaaggca aaaaaggcgg ctggcatgga tctggttttg tggcgtcacg ctgaggcgca
     5401 cgaatggact cagggctgtg acgatatggc gcgaccgctc accgcacgcg gtgaaaggca
     5461 ggcggcacgc atggccacct ggcttgaccg gcagttaccc gaaaacacgc gtatttttgc
     5521 cagcccggcg tgtcgcgccg aacaaaccgc cagtgccctt gatcgaaaat tcaaagtcaa
     5581 acccacactt gggccgatgt gcgttgcctc cgacttgctg gaattggtgc aatggccaga
     5641 tgcaaaaggg acactgcttg tcgtgggcca ccaaccgact ctggggcaag tcatcgccca
     5701 gttgattgga ttgaagcttg cagagtgtcc agtcaggaag ggctcagttt ggtggttgcg
     5761 caatcgccag cgtgagactt tttcacaaac cgtggtggtt gccgtgcaat cgccagagtt
     5821 gctttgattc ccggctgatt taccgcaagc cgacagccaa cggtacctcg caagatgatc
     5881 tcttgtggcg cattgctttt ttcgctagct gcgcacggta accaaactgc cggtagcggc
     5941 cagcatgatg caaacgatgg tcaaccgcag caaggcgttg aggtgctcat cgagcatcaa
     6001 cctgcccagc agtgctgcca ctgctggctg catactggtc atgatcccgg acccttcagt
     6061 tgacttcttt cttgggcact gtgtggacca cgtttccttg tcgattgagc agatacctgg
     6121 ctggaaacct ggtttactgc cccttctgga tttttccggg cgtgcctgac atttaaaatg
     6181 gcatggcaac ttttgtgaac acgacgcatg cagtcctcaa cgccaaaccc ggcaaattgg
     6241 tcaaaatctg aacctggccc ctggtatcgg tggcgcggct acatggtgag atggttgttg
     6301 ttcggcttgg tggtcagtgt cttccagccg gtcgttgatg cccccggcaa gctctggcag
     6361 cacaaactac tccaagcact gattgggctg cttttcgggg cggtctgcgc ggtcgttttt
     6421 acgcctgctg aaaaccgctt caacacgcca cgggtcagtt ggaagtcttg gctactggtg
     6481 gtgatgacct ggctgctggt caaagttgcc tttgtcagca tgctggcaat tgccgatgcg
     6541 cagtaacact gctgagcatc acccaggacc aagctctgac gttccattga ccatcacgtt
     6601 aactttcatg gactccattc acccccggac atgaaagtta tttcacgcta tcgccaaagc
     6661 cagttcggct gcggttgaag tccccctcag gcagatcaat gatgcaatgg attgacttct
     6721 tcagaccggt caacgcccgt gcgctgtgcc tgttgcgatg gtctattttg cggtggtgct
     6781 taaaaaattg atgttgttgg aggtggtttc agttcgaagt acccaaacgg actcagagcg
     6841 cccgacccaa gcgctcaatg ccttcttcga ttcttgccac atcggcggtg gcaaaactca
     6901 ggcggaaagt tgacatatct ggctgatgcg caaaaaaggg cgcgcccggc acaaaggcaa
     6961 cgccatgatc aatggcgcgc ttggcaaaat cggcggcgat gttggtgcgt ccgttggacc
     7021 ccgtcaggcg agcccagagg aacatcccac cctggggcgc gttgaaatca acggcatccc
     7081 ccaactcgcg tttcaagcaa cgcgccatga cctgggcacg cccagcgtaa gttgtccgca
     7141 ccaccgccag cgcatcgccc aagcggttca gcgtcaggta atgggcgcaa atggcttgtg
     7201 tcaggttgct ggtgtgcgcg tcgctgaact gcttgcacat ggtggctttg gccaacagtt
     7261 ccgccggtgc gatcatccag ccaacccgca atcccgggct cagtatcttg ctgaacgaac
     7321 cgcaatgcgc cagccagtca cgactgccgg gcacatcatc gctcaggctc agcaggctcg
     7381 gcggcggcgg ttgatcgaaa aagagctcgc cgtacgggtc atcttccacc accagggttt
     7441 gcgtgcgcac ggcaatttcc agaatctgct tgcgccgctg caggctgagt gtggcgccgc
     7501 tggggttgcc aaacgtgggg atcaggtaaa tcagtttggg ttgatgctct tcaatgagtt
     7561 tttcgagctg gcccacatcc acgccatctg catcaatcgg tgcaccgatg atgtctgcac
     7621 catacagccg aaagcactga atggtggcca gaaatgtcgg cgcttcaacg atcaccttat
     7681 cgccgggcga gatcatggtt ttgccaatca ggtcaagcgc ctgctggctg ccggtggtca
     7741 caatcaggcc gtcaggtgag acgctgcaac ccttggctgc catgaagtcg gagatttttt
     7801 cgcgcagcgg ttgatagcct tcagtcgcac catattgcag taccgggcca gggttgttgg
     7861 ccagcacggc ctgtgaagcc atggcaatgc cttctgcatc aaacagcgcc ggatccggaa
     7921 agccgccagc aaagctgatg atgccaggtt tgccaagcag cttgaaaagt tcacggatgg
     7981 cagaggtctc cacgttttgg aggcggtcgg cgaattgcat ggtgtgtgac tcaacggttg
     8041 gaatgttaaa aacccaaaaa agaggccagg tcgcatcaat agagagtttt cagaatgtgg
     8101 gtgatgttat caagactgcc cctttcctgg ccgcgccaag actgccggtg catctttgac
     8161 tgttggtaca cggttgaagg caagtcagtt gtcgaatctg aacgccacaa aggcacttgc
     8221 cacaaggccg cgatttggac caatgctggt ggtaccagtg ggcgcggcaa gaagcccgtc
     8281 agtggcatgg cgcttagtac catcaaggat atctggctcc tttggctatg caaaaacttc
     8341 gctgccatgt tgcttcagta gacgggatgg gggggtcgaa tgcttgccgg gtgatcctcc
     8401 aaagcggatc catgtttttt gtcctgtttg agttttaagc cgaagttctt ttccaaaaaa
     8461 atgccgagtt tccccaatga aacccacgtt aattttggtt ccgcgttctg tc
//
LOCUS       DAOSLL010000134         8502 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7787_length_8502_cov_3.907541, whole genome shotgun sequence.
ACCESSION   DAOSLL010000134 DAOSLL010000000
VERSION     DAOSLL010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8502
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7787_length_8502_cov_3.907541"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2073
                     /locus_tag="PLB25_07785"
     CDS             <1..2073
                     /locus_tag="PLB25_07785"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004633909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HOE41511.1"
                     /translation="ETNTDDLSPAPDAWSRPDIPLHALAMLKNKRDGITPEDDGKRGP
                     VKFIEDLRARGNLVAYVGDVVGTGSSRKSATNSVLWFTGEDIPFVPNKRFGGVCLGSK
                     IAPIFYNTMEDAGALPIELDVSQMNMGDTIELRPYEGTALKDGKVIANFTLKSDVLFD
                     EVRAGGRIPLIIGRGLTAKARESLGLPVSTLFRLPQNPKATGKGFTLAQKMVGRACGL
                     PEGEGILPGTYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVKMHHELPEFISNRGGIPLRPGDGIIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFGAATGVMPLDMPESVLVRFKGQMQPGITLRDLVNAIPLYAIKSGELTVA
                     KQGKKNVFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCTVYLNKEPIIEYINSNI
                     VMLKWMIASGYSDVRTITRRIKAMEAWLVNPQLLKADDDAQYASVIEIDLADIHEPIL
                     ACPNDPDDVKTLSDVAGTKIDEVFIGSCMTNIGHFRAASKLLENKRDIPVKLWMAPPT
                     KMDAKQLSEEGHYGVLGSAGARMEMPGCSLCMGNQAQVKEGATVFSTSTRNFPNRLGK
                     NSNVYLGSAELAAICAKIGRIPTKAEYLADMNVLTATSSKIYQYLNFDKVQDYTDAAA
                     TVKETASV"
     gene            2231..2878
                     /locus_tag="PLB25_07790"
     CDS             2231..2878
                     /locus_tag="PLB25_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HOE41512.1"
                     /translation="MFFGKLLPRNDDFFGMFNQHADHIVAAAHSFSRLVANYSDHELR
                     QKYHREVDHAEGAADRVTHEVNKAIHKTFITPIDREQIHSLINTMDDVVDLIQDSAET
                     MALYDVHHMTEEISRLTDLSVKCCERVRDAVRLLGKIADHSTAEAALKTCDEIDKLES
                     DADRVMRSAMSKLFREEPDVREVIKLKAIYELLETITDKCEDVANLIEGVILENP"
     gene            2899..3915
                     /locus_tag="PLB25_07795"
     CDS             2899..3915
                     /locus_tag="PLB25_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HOE41513.1"
                     /translation="MQTVQSALWIVVVLVVLAVVFDFMNGFHDAANSIATVVSTGVLK
                     PGQAVIFAAFFNVVAIFVFHLSVAATVGKGIVMPGVVDTYVVFGALIGAITWNFITWY
                     YGIPSSSSHALIGGIVGAAIAKAGVGSLVSAGILKAIIFIFVSPLLGFLLGSLMMVLV
                     AWVFRGFRPLKVDKWFRRLQLVSAGAYSLGHGGNDSQKTIGIIWMLLIATGYSASTDA
                     SPPNWVILVCYAAIGMGTMFGGWRIVKTMGQKITKLKPVGGFCAETGGAITLFLATAL
                     GIPVSTTHTITGAIVGVGSVQRASAVRWGVAGNIIWAWILTIPASAFVAAVAYWLALQ
                     IFPV"
     gene            complement(3924..4445)
                     /locus_tag="PLB25_07800"
     CDS             complement(3924..4445)
                     /locus_tag="PLB25_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HOE41514.1"
                     /translation="MPIIRASQTSDLDQITDIYAHHVLHGTGTFEIAAPSRAEMAQRR
                     EDVLSKQLPFLVATEGQQILGYAYCNWFKPRPAYRHSAEDSIYLAPGSVGVGLGGALL
                     SELARRAEQAGVRKLIAVIGDSANVASIGVHRNLGFSDVGILKSCGWKFDRWLDVVLM
                     DKVLGLGSTMPPD"
     gene            4576..4827
                     /gene="rpsP"
                     /locus_tag="PLB25_07805"
     CDS             4576..4827
                     /gene="rpsP"
                     /locus_tag="PLB25_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HOE41515.1"
                     /translation="MVVIRLARGGAKARPFFNIVVADKRTRRDGRFIERIGFYNPIAK
                     ANEESIRIAQDRLTYWRSVGAQASPTVERLVNQAAEKTV"
     gene            4837..5400
                     /gene="rimM"
                     /locus_tag="PLB25_07810"
     CDS             4837..5400
                     /gene="rimM"
                     /locus_tag="PLB25_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463704.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HOE41516.1"
                     /translation="MLPGLEAAELPADAIEVGRIADAWGIKGWFKVLPYSADPEALFS
                     SRRWYLLSAEKGPKTFSGVGKLAVKEAKTHSDAVVACAHGFDDRNAAESLRSARIFIS
                     RASFPTAHKDEYYWVDLIGLEVINREGQLLGQVRDLLSTGPQTVLLIDYEAEGKLLER
                     MIPFVAVYVDDVDLTARRILVDWQADY"
     gene            5602..6345
                     /gene="trmD"
                     /locus_tag="PLB25_07815"
     CDS             5602..6345
                     /gene="trmD"
                     /locus_tag="PLB25_07815"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HOE41517.1"
                     /translation="MQFDVVTLFPEIFAPFLATGVTRRAFASGQVNVKLSNPRDFAAG
                     NYRRVDDRPFGGGPGMVMLAEPLAQCVTTILAQRSDCAPVLLFSPTGKTLCHDMVSRW
                     ANSLGAVLICGRYEGIDQRFIDTCVTEQISLGDFVLSGGEIAAMALLDAIARLQPGVL
                     NDADSHAQDSFNPLLDGLLDCPHYTRPESWQGSGVPEVLLSGHHERIERWRRDQRLAL
                     TLRLRPELIGQARSQGRLTRQDEALLASL"
     gene            6446..6826
                     /gene="rplS"
                     /locus_tag="PLB25_07820"
     CDS             6446..6826
                     /gene="rplS"
                     /locus_tag="PLB25_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HOE41518.1"
                     /translation="MNLIQILEQEEIARLNKTIPDFGPGDTVIVSLNVVEGTRKRLQA
                     FEGVVIAKRNRGLNSGFTVRKISSGEGVERTFQTYSPLIASIEVKRRGDVRRAKLYYL
                     RDRSGKSARIKEKLPTRKVAVAAA"
     gene            6940..7599
                     /locus_tag="PLB25_07825"
     CDS             6940..7599
                     /locus_tag="PLB25_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HOE41519.1"
                     /translation="MLDPRSVPVLRVDNHLPAVAVTELRPNALRSRFALPPAWQPELR
                     SEPKFMDRQPMQAAVLIPIVMRNEPTVLLTQRTEQMSTHSGQIAFPGGKCDVDDADAT
                     AAALREAFEEVGLESGFVDVLGQLPIYTTGSAFIVTPVVALVTPGFVLKPNAQEVSDV
                     FEVPLQFVMNPANHRHHQVHWQGLQRQWLSMPYLDGATERFIWGATAGMLRNFYRFLI
                     A"
     gene            7625..>8502
                     /gene="ampE"
                     /locus_tag="PLB25_07830"
     CDS             7625..>8502
                     /gene="ampE"
                     /locus_tag="PLB25_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory signaling modulator protein AmpE"
                     /protein_id="HOE41520.1"
                     /translation="MSFISVLFALLLEQAKPLARGNTIHAVMRAWVRWSTQNFDTGKA
                     FHGWLAWSLAVGGPSLVALLVYWLLGTFVGWSLAVLWSALVLYASLGFRQFSSHFTQI
                     RDALAEDDDARAIRLLANWQHIDIREMPRSTILAKVIEFSVLAAHRHVFGVLIWFSVL
                     AAFGLGPTGAVLYRLSEFVVRYWQHQSNAHFQPVSPVLLSNARRAWFLIDWLPARVTA
                     IAFAVMGNFEEAIDGWRRYAQRLDASNDGLVLAATAGAVNIHLSVVGASAEQDAGAQF
                     GPGLDEPAAGCLLPEL"
BASE COUNT         1690 a         2344 c         2461 g         2007 t
ORIGIN      
        1 gaaaccaaca ccgacgattt atccccggca ccggatgcct ggagtcgccc tgatattccc
       61 ttgcatgcct tggccatgct caagaacaag cgggacggta ttacccctga ggacgacggc
      121 aagcgcggcc cggtcaaatt tattgaagat ttgcgtgcgc gtggcaatct ggtggcctat
      181 gttggcgatg tggttggcac cggttcgagc cgcaagtctg caaccaacag tgtgctttgg
      241 tttaccggtg aagacatccc atttgtgccc aacaagcgct ttggtggtgt ttgcctcggc
      301 tccaagatcg ccccgatttt ctacaacacc atggaagacg caggcgcatt gccgatcgag
      361 cttgatgtta gccagatgaa catgggtgac acgattgaat tgcgccccta tgaaggtaca
      421 gccctcaagg atggcaaggt gattgccaac ttcacgctca agagcgatgt cttgtttgac
      481 gaagtgcgcg ctggtggccg gattcccctg atcattggtc gtggcttgac cgccaaagca
      541 cgcgagtcgc tgggtttgcc agtttcaacc ttgttccgtt tgccccaaaa ccccaaggcg
      601 actggcaaag gcttcactct ggcgcaaaaa atggtcggtc gtgcttgtgg cttgcctgag
      661 ggcgaaggca tcctgcccgg cacctattgc gaacccaaga tgaccagtgt cggctcacag
      721 gacaccactg gcccgatgac acgcgacgaa cttaaagacc tggcctgcct gggtttcagc
      781 gccgatctgg tgatgcagtc cttctgccat acggccgctt atcccaaacc ggtggacgta
      841 aaaatgcacc atgaattgcc ggagttcatc agcaaccgtg gtggcattcc tttgcgcccg
      901 ggcgatggca tcatccacag ttggctcaac cgcatgctgt tgcccgacac ggtcggtact
      961 ggcggtgaca gccacactcg tttcccgatc ggcatcagct ttccggcggg ctccggcctg
     1021 gtggctttcg gtgcagcgac aggtgtgatg ccgctggaca tgccggagag cgtgctggtg
     1081 cgtttcaaag gccagatgca gccgggcatt accttgcgtg atctggtcaa cgcgattccg
     1141 ctgtatgcca tcaagtctgg cgagttgacg gtggccaagc agggcaagaa aaacgtgttt
     1201 tcaggtcgaa ttcttgaaat agaaggcttg cccgacctca aggttgagca ggcgtttgaa
     1261 ctcagtgatg cgtctgccga gcgcagtgcg gccggctgta ccgtgtacct gaacaaagag
     1321 ccgatcatcg aatacatcaa cagcaacatt gtgatgctca agtggatgat tgccagcggc
     1381 tactctgatg ttcgaaccat cactcggcgc atcaaggcca tggaagcctg gctggtcaac
     1441 ccgcagttgc tcaaagccga cgacgatgcc cagtatgcct cggtcatcga gatcgatttg
     1501 gccgatatcc atgaacctat cctggcatgc ccgaacgatc cggacgatgt caagactctt
     1561 tcggacgtgg caggcaccaa gattgacgag gtgttcatcg gctcttgcat gaccaacatt
     1621 ggtcatttcc gcgcagcttc caagctgttg gaaaacaagc gtgacatccc ggtcaaactc
     1681 tggatggcac cgcctaccaa aatggatgcc aaacaactca gcgaggaagg gcactatggc
     1741 gtgcttggtt ctgccggagc gcgtatggaa atgcccggct gcagtctgtg catgggtaat
     1801 caggcgcagg tgaaagaggg cgctaccgtg ttctccacca gtacacgcaa tttccccaac
     1861 cggctgggca aaaacagcaa tgtgtatctg ggcagtgctg aactggccgc tatttgcgca
     1921 aaaatcgggc ggatcccgac caaagccgaa tacctcgctg atatgaatgt cttgacagcc
     1981 accagtagca aaatctacca atacctgaat tttgacaaag tacaggatta caccgatgcg
     2041 gccgcaacgg tgaaggagac tgcttcagtt tgaatcggta cctgcgtggc ttgagctagc
     2101 cagttgacca aaaaagaacg cctcacgagt cacatcgtga ggcgttcctt ttgctggata
     2161 gacaaagctg gacggttaaa ctcttggcct gtcgatctgt cacggatttg acacattcca
     2221 aggagttttt gtgttttttg gcaaattgtt accccgcaat gatgattttt ttggcatgtt
     2281 caaccagcat gcggatcaca tcgtcgctgc tgcgcattct ttttccaggt tggtcgcgaa
     2341 ctacagtgat catgaattac gtcagaagta ccaccgggaa gttgaccacg ctgaaggtgc
     2401 tgccgaccga gttacacacg aagtcaacaa ggccattcac aagaccttca tcacgccgat
     2461 tgatcgcgag cagattcaca gcctgatcaa caccatggat gatgtggtgg acctgattca
     2521 ggattctgcc gaaaccatgg ccttgtatga cgtgcatcac atgaccgagg aaatcagccg
     2581 gttgaccgat ttgagcgtca agtgctgtga gcgtgtccgc gatgccgttc ggctgcttgg
     2641 caaaattgct gaccattcaa cggctgaggc cgcgctgaaa acctgcgatg aaatcgacaa
     2701 gctcgaaagt gatgcagatc gcgtcatgcg cagtgccatg agcaagttgt ttcgcgaaga
     2761 gccagatgtg cgtgaggtga tcaagttgaa agcgatctac gaactgctgg agaccatcac
     2821 tgataagtgc gaggatgttg ccaatttgat cgagggcgtg atcctcgaaa acccctgatc
     2881 agccatggga cccggacaat gcaaactgtt caatcggctc tgtggatcgt tgttgtgttg
     2941 gtggtactcg ctgtcgtatt tgatttcatg aatggctttc atgatgcggc aaactccatc
     3001 gcgacggtgg tttccaccgg tgttcttaag cctggccagg cggtaatttt tgcggctttt
     3061 ttcaatgtgg tggccatttt tgtctttcac ctgagtgttg ccgcaactgt tggcaaaggc
     3121 attgtgatgc ctggtgtggt ggacacctat gtggtcttcg gtgccctcat tggtgccatc
     3181 acctggaatt tcatcacttg gtactacggc attccaagta gctcgtctca tgcgctgatt
     3241 ggcggcatcg ttggtgcagc gattgccaag gcgggtgtcg gttctctcgt gtcggcgggc
     3301 attttgaaag cgatcatctt catttttgtc tcgccattgc tggggttctt gcttggttcg
     3361 ttgatgatgg tgctggtagc ctgggttttt cgtggctttc gacccctcaa agttgacaag
     3421 tggtttcgac gtctgcagtt ggtgtcagcc ggggcctaca gtttggggca cggcggcaat
     3481 gactcgcaaa aaaccatcgg catcatctgg atgctgttga ttgccacagg gtattcggcc
     3541 tcaaccgatg cgtcgccgcc gaactgggtg attctggttt gctacgccgc gattggcatg
     3601 ggtaccatgt ttggcggctg gcgaatcgtt aagacgatgg ggcagaagat caccaaactt
     3661 aagccggtgg gtggcttctg cgcggaaacc ggtggtgcca tcactttgtt ccttgctacc
     3721 gcgcttggca tacccgtgtc caccacccac accatcacgg gtgcgattgt gggcgttggc
     3781 tcagtccagc gcgccagtgc tgtacgctgg ggtgtcgctg gcaacatcat ctgggcctgg
     3841 atcctgacga ttccggcgag tgcctttgtc gctgcagtgg cctactggct agccctgcag
     3901 atctttccgg tctgaagcct gcgtcaatcg ggtggcatgg tcgaacccaa gcccagaacc
     3961 ttgtccatca acaccacgtc gagccaccgg tcaaacttcc agccgcatga cttcaaaatg
     4021 ccgacatcgc tgaaaccgag gtttcggtgg acaccgattg aagcgacgtt ggccgagtcg
     4081 ccgatgaccg caatgagctt tctgaccccc gcttgctcgg cacgcctggc gagctcgctc
     4141 aacaaggcac cacccaagcc gactcctacg gacccaggcg caaggtaaat cgagtcttca
     4201 gcagaatgcc ggtaagcggg gcggggcttg aaccagttgc agtaggcata gcccagtatt
     4261 tgctgtcctt cggtggccac taaaaaggga agttgtttgc tcaaaacgtc ctcgcgccgc
     4321 tgggccatct cagcgcggct gggcgcggca atttcaaaag tgccggtgcc atgcaacaca
     4381 tggtgtgcgt atatatcggt aatctggtct aggtcgctgg tctgactggc tctgatgatt
     4441 ggcatgattt ttctgctggc tataatgttg ggttgtctgg cgtgccgctg acctggtggt
     4501 caagttgcgt gtctcaaacg tcgggctaag tgccaatttc gtggaaataa aacgccacta
     4561 cccaaaggat aaattatggt cgtcattcga ctcgcccgtg gcggcgcaaa agctcgcccg
     4621 tttttcaata ttgtcgttgc cgacaagcgt acccgtcgtg atggccggtt tatcgaacgc
     4681 atcgggtttt acaaccccat cgccaaggcc aacgaggaga gcattcgcat agcgcaggat
     4741 cgcctgactt actggcgcag tgtcggtgcc caggcttccc ccaccgtgga gcggctggtc
     4801 aaccaggctg ccgaaaaaac agtttgattt ctgtctatgt tgcccggcct tgaagctgcc
     4861 gaactgccgg cagatgccat cgaagttggg cgcatagctg atgcctgggg catcaagggg
     4921 tggttcaaag tactgcctta cagcgccgat cccgaggcgc ttttttcttc cagacgctgg
     4981 tacttgttgt ccgccgaaaa aggtcccaaa accttttcgg gcgttggcaa actggccgtc
     5041 aaggaagcca aaacccattc tgatgcggtg gtcgcctgtg cccatgggtt tgatgacaga
     5101 aatgccgctg aaagcctgcg tagtgcgcgc attttcattt cccgagccag tttccccacg
     5161 gcacacaaag acgaatacta ctgggttgat ctgatcggcc ttgaggtgat caaccgtgaa
     5221 ggccaattac tgggccaagt gcgtgatttg ctctcaaccg gtccgcaaac cgtgctgctg
     5281 attgactacg aggctgaagg taagctgctg gagcggatga tcccgtttgt cgcggtctat
     5341 gtggatgatg tcgatctgac tgcgcggcgg attctggtgg attggcaagc cgactattaa
     5401 gaacatggtc atcaatgact tccccctttt gctcgtcgag ttcgggctca ctgcggcgct
     5461 gcaaatcagt ggtatggctt gccacatttc aaatggaccc tgacgggtta gtttgagggc
     5521 gcgctttgcg cctggcattg cagcccagat ccaacggtca aatgcgtcgc gtgtttttgc
     5581 ccctatccga agtgccacgt catgcaattt gatgtcgtca cgctgtttcc cgaaattttt
     5641 gccccttttt tggccaccgg ggtaacccgg cgagcttttg cgtccggtca ggtcaatgtc
     5701 aaactaagca acccgcgtga ttttgccgct ggcaattacc gccgtgtcga tgaccggcct
     5761 ttcgggggcg gccctggcat ggttatgctg gccgaacccc tggcccaatg cgtgaccacc
     5821 attcttgcgc agcgcagcga ctgcgcaccg gtgttgttgt tttcgccaac cggcaaaaca
     5881 ctgtgccacg acatggttag ccggtgggcc aacagcttgg gggcggtgtt gatttgcggg
     5941 cgttacgagg gcattgacca gcgcttcatt gacacctgcg tcactgagca aatcagtctt
     6001 ggtgattttg tcctgtctgg cggtgaaatt gccgccatgg cgctgctcga tgcgattgcg
     6061 cggttgcagc ctggcgtgct caacgatgcc gacagccacg cgcaggacag cttcaaccca
     6121 ctgctggacg gtttgctgga ctgcccgcat tacacccggc cggaaagctg gcagggcagc
     6181 ggtgtgccag aggtgctgtt gtcgggtcac catgaacgca ttgagcgctg gcggcgtgac
     6241 cagcgtctgg ccttgacatt gcggctgcgc ccggaattga tcggtcaagc ccgctcgcaa
     6301 ggtcggctaa cccgccagga tgaagccttg ctagcctcgc tataatggcg ggttaaccga
     6361 tcctctgcag ggccgctttg gtgtttgaca tccgggcatg tgccaattct ggcgctggcg
     6421 cttgcaagat cattcgaggt cattcatgaa tctgattcaa atccttgagc aagaagaaat
     6481 tgctcgctta aataaaacca ttcccgactt cggtccaggc gacaccgtca tcgttagcct
     6541 caacgtggtt gaaggcactc gcaagcggct gcaggccttt gaaggcgtgg tgattgccaa
     6601 gcgcaatcgg ggtctcaaca gcggctttac ggtgcgcaag atttcaagcg gtgaaggtgt
     6661 cgagcgcacc ttccagacct acagcccgct gattgccagc attgaagtca agcgccgtgg
     6721 tgatgtccgt cgtgccaagc tgtattacct gcgcgatcgc agcggcaagt cggcgcgcat
     6781 caaggaaaag ctgccgaccc gcaaggtggc ggtagctgct gcgtaacacg tcagtcgctt
     6841 aagaaaaacc gccttctgaa cgcagtggcg gttttttttc gcctgcaatt tgtttacttt
     6901 gggacttcaa gccaggtcat gtcaacaccc agattgccca tgcttgaccc acgttcggtc
     6961 ccagtgcttc gcgtggacaa ccacttgccg gcggttgcgg tcaccgaact gcggcccaac
     7021 gcattgcgtt cgcgctttgc attgccaccg gcctggcaac cggagctgcg ctctgagccc
     7081 aagttcatgg accgccaacc gatgcaggcc gctgtgttga tccccatcgt gatgcgcaat
     7141 gagcccaccg ttttgctgac gcagcgcacc gaacaaatgt ccacccattc cgggcagatc
     7201 gcctttcctg gcggcaaatg cgacgtcgat gatgccgatg ccacggccgc tgcgctgcgc
     7261 gaagccttcg aggaggtcgg tcttgagagc ggttttgtcg atgtgctggg gcagttgccg
     7321 atctacacca cgggttcggc gttcatcgtc acgccggtgg tggcactggt cacaccaggc
     7381 tttgtgctca agcccaatgc ccaggaggtt tctgacgtgt ttgaggtgcc cttgcagttc
     7441 gtcatgaacc cggccaacca ccgccatcac caagtgcact ggcagggcct gcagcggcag
     7501 tggctgtcga tgccctacct tgatggtgca actgagcgct tcatctgggg tgccacggcc
     7561 ggcatgctgc gcaactttta ccggtttttg atagcctgac tgacactacc gctatgatca
     7621 ttgcatgagc ttcatttcgg tcctattcgc cctgttgctg gaacaggcca agccgttggc
     7681 gcgcggaaac accatccacg ccgtcatgcg tgcctgggtg cgctggtcca cgcagaattt
     7741 tgataccggc aaggcctttc atggctggct ggcctggagc ttggctgtag gtggtccgtc
     7801 gctggtggcg ctgctggttt actggctgct gggcaccttt gtcggctggt cgctggccgt
     7861 gctctggagc gcgctggtgt tgtatgcctc gctcgggttt cgtcagttca gttcgcattt
     7921 cacccaaata cgcgatgccc tggccgaaga cgacgatgcc cgcgccatcc gtttgctggc
     7981 caactggcag cacatcgaca ttcgcgaaat gccgcgcagc acgattcttg ccaaggtgat
     8041 tgagttttct gtgctggctg cgcaccggca cgtgtttggt gtgctgatct ggttttcggt
     8101 gctggcagcg tttggcctgg gacctaccgg ggccgtgctg taccgtttga gcgagtttgt
     8161 cgtgcgttac tggcagcacc aaagcaacgc gcacttccag ccggttagcc ccgtcttgct
     8221 gagcaatgcc cgccgcgcct ggttcctgat tgactggttg cccgcgcgtg tcactgccat
     8281 tgcctttgcc gtcatgggca actttgagga ggccattgac ggctggcgcc gctatgccca
     8341 gcgcctggat gccagcaatg atggcctggt attggctgcc acggcgggcg cggtcaatat
     8401 ccatttgtca gttgttggcg cctcagcaga gcaggacgca ggcgcccagt ttggtcccgg
     8461 cctggacgaa cctgccgcgg gctgcctgtt gccggaactg ca
//
LOCUS       DAOSLL010000135         8366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_7952_length_8366_cov_4.506437, whole genome shotgun sequence.
ACCESSION   DAOSLL010000135 DAOSLL010000000
VERSION     DAOSLL010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8366
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7952_length_8366_cov_4.506437"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            159..1094
                     /locus_tag="PLB25_07835"
     CDS             159..1094
                     /locus_tag="PLB25_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE41521.1"
                     /translation="MSITLRRSLLLAAGLTVLLAATAPAQAQATAAVRVGSKIDTEGK
                     LLGNVIVLALEANGIKVDNRVATGNTKVVRTALLAGEIDIYPEYTGNGAFMLGEEGNT
                     AWRGLRSGFELVKKMDFERNKLVWLEPANANNTWAIAVRKDIATTHQLHTLEDLAKFA
                     AGPGPLKLAASAEFMERADAMPAFTAAYGLKLRPEQTLILAGGDTAATIRAAAEKTSG
                     VNAAMAYGTDGPVAALGLVIMQDPKGAQPVYAPAPVIRDEVLKRHPQIAKLLEPIFRS
                     MKTETLQRLNAQIQLEGLDARKVAGDYLRSKGFIK"
     gene            1107..2342
                     /locus_tag="PLB25_07840"
     CDS             1107..2342
                     /locus_tag="PLB25_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE41522.1"
                     /translation="MPQQPVLALCSALCLWAALALPLVRVAPNRLLSGQPVYLADLLP
                     HGGWLTLALLPVLTMLALVAWPWLRARSRQPPPLRQAVDPPPWLTPLLLLTMSLTLAG
                     LLWLAGGHASLIAQTESRLARTSLGAGFWSLLALIWLMCQDALSHSQRSDAWRAALLA
                     LVAAPSMVMIWLGCGDDLSIMKEYANRSDAFWQALLRHLQIVSVATLPSVLLGLPLAW
                     GCSRSGALQRLLFPLLNIIQTIPSIALFGLFMAPLAWAATRYPALAQAGISGVGMAPA
                     VLALLLYSLLPIVRSALAGLQQVPAAATTAARAMGLSGWQIFYQIEVPLALPVLLPGL
                     RTAVVQTIGLAAVTALVGAGGLGALMFDGLFSAANELVLLGVLPIVLLAMLADALFKG
                     LTALAGHGTASAAANGAVR"
     gene            2339..3280
                     /locus_tag="PLB25_07845"
     CDS             2339..3280
                     /locus_tag="PLB25_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE41523.1"
                     /translation="MIDFQGVSKAYDGVLAIDRLDLHIAKGELVVLIGPSGSGKSTAL
                     KLINRMLDHDSGRILFEGREIYSFDVRDLRRRMGYAIQSVGLFPHWTVERNIATVPHL
                     LGWPAARIADRVTELLTLFHLEPALYRQRYPHQLSGGQQQRIGVARALAADPAVLLMD
                     EPFGALDPVTRAALQLELKRIHQSFGKTIVLVTHDIDEALLLADKIVLLDQGRVVQIG
                     TPLALLAQPASEFVPDFFGRADLGIKLLSLKPVAALVRPGPAAPGSPSVINSATLREA
                     VASLAAHCVAVLNVVDAHNTVLGSLHAADVFAPERHG"
     gene            3273..4064
                     /locus_tag="PLB25_07850"
     CDS             3273..4064
                     /locus_tag="PLB25_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE41524.1"
                     /translation="MAEPVMVADRSAGRSARRSVWAGLLQDRLLWVLALLLALSYGLP
                     HLHAVMAALFPAQQRPVYLQEPLATLLWQHIALVLVSSAAATLLGSAAGVAVSRRAGQ
                     AFRPMLESIATTGQTLPPIAVLALAVPMVGFGELPALIALALYGLLPVLQATLAGLAG
                     VPQAVLESARAMGLSARQVLWQVEVPLALPVWLAGVRSSVIINIGTAAIASTVGAKTL
                     GSPIIVGLSGFNTAYVLQGAVLVGLLAVATDMAFERLAQRLAWQV"
     gene            complement(4230..4394)
                     /locus_tag="PLB25_07855"
     CDS             complement(4230..4394)
                     /locus_tag="PLB25_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41525.1"
                     /translation="MSRKLMYQGWQPASGNCPAQICRVGSAGSPVLVLVEQGILIPRD
                     EPKQAGQALP"
     gene            complement(4597..6006)
                     /locus_tag="PLB25_07860"
     CDS             complement(4597..6006)
                     /locus_tag="PLB25_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018913212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HOE41526.1"
                     /translation="MHEIICPHCNKAFKIDEAGYADILKQVRDGAFEQQLHERLELAE
                     QDKRNAVELAQVKVAGEMQKSAVAKDSEIQELKARLDAAEVARKLAITEALSAVQKER
                     DALANELQQAKRDRVAAAELAEAKLANGLQKAAADKDAEIQGLKAKLESTDVIQKLAI
                     TEAVNAVERERDELKNGLALAELEKQLAEKSLKDKFETQIKDRDDAIERLRDMKARLS
                     TKMVGETLEQHCETEFNRIRATAFPRAYFEKDNDARTGSKGDYIFRDLDESGTEIVSI
                     MFEMKNENDRTSTKNRNEDFLKELDKDRLEKGCEYAVLVSLLEPDSELYNTGIVDVFH
                     RFPKMYIVRPQFFLPIITLLRNAAMNSLKYKSELALVRAQNIDITNFEASLDTFKTAF
                     ARNYDLASNSFKKAIDEIDKSIDHLQKTKDALLGTDRNLRLANDKAQDVTIKKLTRGN
                     PTMAAKFAELKNPGLSDAE"
     gene            complement(6242..6532)
                     /locus_tag="PLB25_07865"
     CDS             complement(6242..6532)
                     /locus_tag="PLB25_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41527.1"
                     /translation="MRSIIEEAIRNRRYLTFIYKGIARQVQPVAVGVSRAGNEVLRCF
                     QTKGAHNTDGHEWNLCTLSEISGLAVTGEKFGAPPPGYNRGDKGMVKIYAEL"
     gene            complement(6850..>8366)
                     /locus_tag="PLB25_07870"
     CDS             complement(6850..>8366)
                     /locus_tag="PLB25_07870"
                     /inference="COORDINATES: protein motif:HMM:NF024565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE41528.1"
                     /translation="VFNPNKAETVFDTLDRQGFFRGGHLVQLRGELGPIGKEALETKL
                     KAIHSRIDEDEALKRLRVNLAKNAQTQALVQLIENLSTVEVDFLLERIKPANQAKFRQ
                     ALWLYYLQDNKDATSYIECYTESKDELQRIEALAATAVPQWTQAVELFNDRFLDMPFT
                     LSVPNQVQAALGKESAKLRFTFKDGPDTVEWSRQEVKTLSQGERRALYLLNFIFEVEA
                     RKLRQQETIFIIDDIADSFDYKNKHAIIQYLYDIGQIPYFRQIILTHNFDFFRALSDS
                     FVHRDRCLMTNRGQVKICLTKADGISNYFIGMWKNNVANDDGILCATIPFTRNLIEYT
                     KGEADADYLELTYLLHWKERTSVITVGTYLDIYNRLFRTAHDTSSTVIIKDLIFTKAE
                     SICQKLNHDGLNLADKVLLSIAIRLRSEVFMTEALRVIKNDAGYWCQSNNQFGALIKE
                     FSALEPTSPALRVLGKVSVTVSSNIHLNSFMYEPILDLSLDHLIKLYREVENLT"
BASE COUNT         1627 a         2351 c         2371 g         2017 t
ORIGIN      
        1 gctggctaaa caagcgtctt gcaacgacgc tgtgcgccca gatccgccgg tgcaagtagg
       61 cgaagttatt tctgaccaca tccttatcct agtcaccact ccttgcgagc atgcggctgc
      121 acagcctgac tgatgtactt tttatgagag gtttagctat gtcgatcacc ttgagacgtt
      181 cccttttgct ggccgctggc ctgacagttc tgctggctgc caccgcgcca gcgcaggcac
      241 aagccactgc cgcggtgcgg gttggctcca aaattgacac cgaaggcaag ctgctcggca
      301 atgtcatcgt gctggcactg gaggccaatg gcatcaaggt ggacaaccgg gtcgccaccg
      361 gcaacaccaa ggtggtgcgc accgcgctgc tggccggcga aattgacatc taccccgaat
      421 acaccggcaa cggtgccttc atgctcggtg aagaaggcaa cactgcctgg cgcggcctgc
      481 gcagcgggtt tgagctggtc aagaagatgg acttcgagcg caacaaactt gtctggctgg
      541 agccggccaa tgccaacaac acctgggcga ttgcggtgcg caaggacatc gccacgacgc
      601 atcaattgca caccctcgaa gatctggcca aatttgccgc cggccccggc ccactcaagc
      661 tggccgcatc tgccgagttc atggagcgcg cggatgcgat gccggccttt accgctgcct
      721 atggcctgaa gctgcgcccg gagcaaaccc tgattctggc tggtggcgac accgctgcca
      781 cgatccgggc cgctgctgaa aaaacctcgg gtgtcaatgc cgccatggcc tatggcaccg
      841 acggcccggt ggctgcgctg ggcctggtca tcatgcaaga ccccaaaggc gcgcagccgg
      901 tttatgcacc ggccccggtg attcgcgacg aggtgctcaa acgccacccg cagatcgcca
      961 aactgctgga gccaattttc agatccatga agaccgagac gctgcagcgg ctcaacgccc
     1021 agattcagtt ggaaggactt gacgcgcgca aggtcgctgg cgactacctg cgcagcaaag
     1081 gcttcatcaa gtaaagcgac atcggcgtgc cgcagcaacc ggttctggcc ctgtgcagcg
     1141 cgctgtgcct gtgggctgcg ctggccttgc cgctggtgcg cgtggcaccc aaccggctgc
     1201 tgtcgggcca gccggtgtat ctggccgact tgctgcccca cggcggctgg ctgacgctag
     1261 cgctgctgcc agtgttgacc atgctggcgc tggtggcctg gccatggttg cgggcacggt
     1321 cacgccagcc gccgcccttg cgtcaagctg ttgatccgcc gccttggctc acgcccctgc
     1381 tgctgttgac catgtcgtta acgctggctg gcctgctttg gctggcgggc gggcatgcca
     1441 gtttgatcgc acaaaccgaa tccagactgg cgcgcacctc gctgggggca ggattctgga
     1501 gcctgctggc gctgatctgg ctgatgtgtc aggacgcgct cagccacagc cagcgctctg
     1561 atgcctggcg tgcggcgctg ctggcgctgg tggcagcacc gagcatggtc atgatctggc
     1621 tgggttgcgg cgatgatttg tcgatcatga aggaatacgc caaccgcagc gacgcatttt
     1681 ggcaagcgct gctgcgccac ctgcaaatcg tcagcgttgc caccctgccc tcggtgctgc
     1741 tcggtttgcc gctggcctgg ggttgcagcc gctctggcgc tctgcaaagg ctgttgtttc
     1801 cgctgctcaa catcatccag acgattccgt caattgccct atttggcttg ttcatggcac
     1861 cgctggcctg ggccgcaacg cgctatccgg cgctggcgca ggctggcatc agtggtgttg
     1921 gcatggcacc ggcggtactg gcactgctgc tctacagctt gctgccgatt gtgcgatcag
     1981 cactggccgg gctacagcag gtgcccgcag cggccaccac ggccgcgcgg gccatgggcc
     2041 tgtcgggctg gcagattttt taccagattg aagtgcctct ggcgctgccg gtgttgctgc
     2101 cgggcttgcg caccgctgtg gtgcagacga ttggcctggc ggcggtgacc gcgctggtcg
     2161 gtgctggcgg gcttggggca ctgatgtttg acggcctgtt cagcgctgcc aatgagctgg
     2221 tgctgctggg tgtgttgccg attgtgctgc tggcgatgct ggccgacgcg ctgttcaagg
     2281 gtttgacggc gctggcaggc catggcacgg caagcgccgc agccaacgga gctgtgcggt
     2341 gatcgacttt cagggtgtca gcaaagccta tgacggcgtg ctggcgattg accggcttga
     2401 cctgcacatt gccaagggcg aactggtggt gctgattggc ccgtcaggtt cgggcaaaag
     2461 caccgcgctg aaactgatca accgcatgct cgaccacgac agcggccgca ttttgttcga
     2521 aggccgcgaa atctacagct ttgacgtgcg cgacctgcgc cgccgcatgg gctatgcgat
     2581 ccagtcggtc ggcctgttcc cgcactggac cgttgaacgc aacattgcca ccgtgccgca
     2641 cctgctgggc tggcctgccg cgcgcatcgc cgaccgggtg accgagctgc tgaccctgtt
     2701 tcacctggag ccagcgctgt atcgccaacg ctacccgcac cagttgtccg gcgggcaaca
     2761 gcagcgtatc ggggtggcgc gggcgctggc ggccgacccg gcggtgctgc tgatggacga
     2821 gccctttggc gcacttgacc cggtgacgcg cgccgcgctg caacttgaac tcaagcgcat
     2881 ccaccagagc tttggcaaaa ccattgtgct ggtcacccat gacatcgacg aagccctgct
     2941 gctggctgac aagatcgtgc tgcttgacca ggggcgcgtg gtgcagatcg gcacgccgct
     3001 ggcgctgctg gcgcaaccgg ccagtgaatt tgtgcccgat ttctttggcc gcgctgacct
     3061 gggcatcaag ctgctgtcgc tcaagccggt ggccgcgctg gtgcggccag gcccggcagc
     3121 acccggcagc cccagcgtga tcaacagcgc gacgctgcgc gaagccgtgg ccagcctggc
     3181 agcccactgc gtagcggtgc tcaatgtggt ggacgcgcac aacaccgtgc tgggcagcct
     3241 gcacgctgcc gatgtgtttg cgcccgagcg ccatggctga accggtcatg gtggcggaca
     3301 ggtcggcggg cagatcggcg cgccgcagtg tctgggcagg cctgttgcaa gaccggctgc
     3361 tgtgggtgct ggcgctgctg ctggccctga gctacggctt gccgcaccta cacgccgtaa
     3421 tggccgcctt gtttccggca caacaacgcc cggtttacct gcaagagccc ctggctaccc
     3481 tgctgtggca acacatcgcc ctggtgctgg tgtccagtgc cgccgccaca ctgctgggca
     3541 gcgccgccgg ggtggcagtc agccgccgtg ccggtcaggc ctttcgaccg atgctggagt
     3601 cgattgccac cactggccag accttgccac cgattgcggt gctggcgctg gcagtgccga
     3661 tggtcggttt tggtgaactg ccagcgctga tcgcgctggc cttgtatggg ctgctgccgg
     3721 tgctgcaagc gaccctggcc gggctggctg gcgtgccgca ggcggtgctg gaatcggccc
     3781 gcgccatggg cctgagcgcc cggcaagtgc tgtggcaggt ggaagtaccg ctggcgctgc
     3841 cggtatggct ggccggggtg cgcagctcgg tcatcatcaa cattggcacc gcagcgattg
     3901 cctcgaccgt tggggccaaa acattggggt cgccgatcat tgtcggcctg agcggcttca
     3961 ataccgccta tgtgctgcag ggtgcggtgc tggtgggttt gctggcagtg gcgaccgaca
     4021 tggcgttcga gcggctggcg cagcggttgg cctggcaggt ttgaggctgc cgggcggtgg
     4081 cgctgggttt accgctcagg cgagcatgcc cgcgtctgag catttttata acaaattggc
     4141 ctctaacctt tattacatat gcgctggcag ctattattaa tatagcaaat atggccgtcg
     4201 catggccgat gtgaagccaa cattcagttt cacgggagcg cttgaccagc ctgctttggc
     4261 tcatcgcgag gaatcaggat gccttgctcg accagcacca gcaccggcga cccggcactg
     4321 ccaacacggc agatttgggc tggacaattg ccgctggcag gttgccaacc ctggtacatc
     4381 agttttctgc tcattgacct gcaagtgaaa cttcccatac tgccttctca agaagccaag
     4441 cacccgcccg cccgcacttt gactgcgcag gtaagcgtca aaacaggctg tagcccttgt
     4501 ggaatgtgcg caagcagcta tcacaataag agcaattcac tctgcggctc ggctcagcgc
     4561 gatcaagcct ccctcatcag gcagccacaa cacgcctcat tcggcatcgg aaagaccagg
     4621 gttcttaagc tcggcgaact tggctgccat cgtcgggttg ccgcgtgtca gtttcttgat
     4681 cgtcacgtct tgcgccttat cgttggcaag acgcaggttc cggtcggtgc caagcagtgc
     4741 gtccttggtt ttctgcaggt ggtcgataga tttatcgatc tcatcgatgg cctttttgaa
     4801 gctgttggag gccaggtcat agttgcgcgc gaatgcggtc ttgaaggtat cgaggctggc
     4861 ctcaaaattc gtgatgtcaa tgttctgcgc cctgaccagc gccagctccg atttgtactt
     4921 gagcgagttc atcgctgcat tgcgcagcag cgtgatgatg ggcagaaaga actgggggcg
     4981 gacgatgtac atcttgggga agcgatggaa cacatccacg atgccggtgt tgtacagctc
     5041 actgtctggc tccagtaggg agaccagcac ggcatattcg caccccttct caaggcgatc
     5101 cttgtccagc tctttcagaa agtcctcgtt cctattcttg gtgctggtgc ggtcgttctc
     5161 atttttcatt tcgaacatga tggagacgat ttcggtgcct gactcgtcca ggtcgcgaaa
     5221 aatgtagtcg cccttgctgc cggtgcgcgc atcgttgtct ttctcgaaat aggcgcgcgg
     5281 aaacgctgtg gcgcgaatgc ggttgaactc cgtctcgcag tgctgctcca gcgtctcgcc
     5341 aaccatcttg gtggacaggc gagccttcat gtcgcgcagg cgctctatcg catcatcgcg
     5401 atccttgatc tgcgtctcaa acttgtcttt gagggatttt tctgcgagct gcttttcaag
     5461 ttcggccagc gccaggccgt tcttcagctc gtcacgttcc cgctcgactg cattcacagc
     5521 ttcggtgatc gcgagctttt gtataacgtc agtggactcc agcttggcct tcaacccctg
     5581 gatttcggca tccttatccg ctgcggcttt ctgtagcccg ttggcgagtt tggcctcggc
     5641 cagttctgcc gcagctactc tgtcgcgctt ggcttgctga agttcgttgg cgagtgcgtc
     5701 acgttccttt tgcaccgcac tgagagcctc ggtgatggcg agtttgcgcg cgacctctgc
     5761 cgcatccagc ctggctttca gttcctggat ttcggaatcc ttggcgacag cagacttctg
     5821 catttcgcca gccactttga cctgcgccag ctcgacggca ttgcgcttgt cttgctcggc
     5881 cagttcaagg cgttcatgca actgctgctc gaacgcgccg tcgcgcactt gtttcaggat
     5941 atctgcgtac cccgcctcat cgattttgaa tgctttgttg cagtgggggc agatgatttc
     6001 atgcatgtcc gtgtttcctt ttttcgtttg ttgtcggcca atcggtcatc tgggcaaacg
     6061 caattgcttg gtaaatcgat cctggccgct ttttttctaa cgcctcatga cgccaagtgt
     6121 tcaggttgta gaaattggtc acgatgccga tacgatgttt gcaccgagta tggtgccagc
     6181 ccgcctaaat actgcaccga gagtcccgta ttcacttggg atgctctcgt agttttcgac
     6241 cttaaagctc agcgtaaatt ttgaccatcc ctttgtctcc gcggttgtac cccgggggtg
     6301 gcgctccgaa tttttctcca gttaccgcaa gacctgatat ctcgcttaag gtacacaaat
     6361 tccattcatg accatcggtg ttgtgcgcac cttttgtctg aaagcaacgc aatacttcat
     6421 tgccagcgcg tgaaacgccg acagctacgg gttgtacctg ccgtgcgata cctttgtaga
     6481 taaatgtcag atagcggcga ttccgaattg cctcttcaat aatgcttctc atagtgcatc
     6541 cccaaaaaaa aatagatcct agtacatcat tcaacaattc aatgttcaaa tacagggcgg
     6601 ccagacattc aaggatttac acctaatttc tgcaagtagt gtctttgcaa tgactcactc
     6661 tatcaacaac acggacacaa atgtgcattt cctcaaaatc agagcggaga agagtcagat
     6721 ttttgtaatg gttggtgtaa ggtcgctagc aatcgcaaat atctacgtaa gctatcaatt
     6781 ggttgattca gagttgaccg aatagaacac tcgggtcctg ttgactgaat tgagacctga
     6841 agctgaaaac tacgttaaat tctcgacttc tctatatagt ttaataagat ggtcgagtga
     6901 taaatcgagg attggctcat acataaacga gttaagatgt atatttgagc tcacagtaac
     6961 gcttaccttg cctagtactc gcaatgctgg cgatgtcggc tccaatgcac tgaattcctt
     7021 tattagagca ccaaattggt tattggactg acaccagtaa cctgcgtcgt ttttgatgac
     7081 gcgaagtgct tcggtcataa aaacttccga tcgtagacgt attgcgattg ataacagaac
     7141 tttgtcagcc aagttcaaac catcatggtt aagcttttga caaatgctct cggcttttgt
     7201 gaaaatcaaa tcctttataa tcactgtact actggtgtcg tgtgcagtgc gaaaaagtcg
     7261 attatatata tctaaatacg tgccaacggt aattacgcta gtgcgttctt tccagtgaag
     7321 taaatatgta agttccaaat aatcggcatc tgcttcaccc ttggtgtact cgattagatt
     7381 tcgagtaaat gggatggttg cacacaagat accatcatca tttgcgacat tgtttttcca
     7441 catgccgata aagtagttgc taattccatc agccttggtg aggcaaattt tcacctgccc
     7501 gcgattggtc attagacatc tgtctctatg aacaaaacta tctgaaagtg cacgaaagaa
     7561 gtcgaagtta tgggtaagaa ttatttggcg aaagtatgga atttggccaa tatcatataa
     7621 atactgaatt attgcatgct tgtttttata gtcgaatgag tctgcaatat catcaataat
     7681 aaaaatagtt tcttgttggc gtagctttct tgcctctact tcaaagatga agttcaaaag
     7741 atagagcgcc cgcctttcgc cttggctgag agtcttaact tcttgacgtg accattcgac
     7801 tgtatctggg ccatctttaa atgtgaacct tagttttgca ctttctttgc caagcgccgc
     7861 ttgtacctga ttagggaccg agagcgtaaa gggcatgtcc agaaaccgat cattgaaaag
     7921 ctccacagct tgtgtccact gcggaacagc agtggcggcc aatgcctcga ttcgttgtag
     7981 ttcgtcttta ctttccgtgt agcactcgat gtatgaggtt gcatccttgt tatcttgaag
     8041 atagtaaagc caaagcgctt gacgaaattt ggcttggttc gctggcttaa ttctttcaag
     8101 aagaaaatcc acttcaactg ttgataggtt ttcaatcaac tgtacaagtg cttgtgtctg
     8161 tgcattcttt gccaaattta cacgaagtct tttcagcgct tcatcttcat ctattcgaga
     8221 gtgaattgct ttgagtttgg tttcaagagc ttcttttcca ataggaccca attctccgcg
     8281 caactgaacc agatgccctc ccctaaaaaa accttgtcga tctagggtat caaatactgt
     8341 ttcagctttg tttggattga aaacat
//
LOCUS       DAOSLL010000136         8323 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8003_length_8323_cov_3.739115, whole genome shotgun sequence.
ACCESSION   DAOSLL010000136 DAOSLL010000000
VERSION     DAOSLL010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8003_length_8323_cov_3.739115"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(42..1055)
                     /locus_tag="PLB25_07875"
     CDS             complement(42..1055)
                     /locus_tag="PLB25_07875"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HOE41529.1"
                     /translation="MKPAVLVARAVFPQVLANLAEHFEVRSNQLDEVWTQAQLISQLQ
                     GKQGVLTTGSERIDAALLVACPGLKICANMAVGFNNFDLDAMTAAGVLASNTPDVLTD
                     TTADFGFALLLATARRMAEAEHFLRAGLWSRWRYDMFAGAEVHGSTLGILGMGRIGRG
                     IARRAAHGFGMKVIYHNRSRLDAATEAACAARWVSKNELLSTCDHLMLVLPYSQQAHH
                     AIGAPELALMKPTATLVNIARGGIVDDAALAAALRQGQLAAAGLDVFEGEPAVHPDLL
                     ALANVVLTPHIASATLATRLAMANLAADNLIGYLVHGKALTPLNLDSSVDRLFSSVSP
                     HEY"
     gene            complement(1062..2348)
                     /gene="nhaD"
                     /locus_tag="PLB25_07880"
     CDS             complement(1062..2348)
                     /gene="nhaD"
                     /locus_tag="PLB25_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter NhaD"
                     /protein_id="HOE41530.1"
                     /translation="MLAILSIVFVLAYAAIALEHQIKINKSASALIGAGLLWTIYAMM
                     GSDVKLLEEHLGASLMGTAQIVFFLMGAMTIVEVVDAHNGFVVITSRIKTTKLSTLMW
                     MVGFVAFFLSSILDNLTTTIVMVSLMKKLLDQREDRLFFAGIIVIAANAGGAWTPIGD
                     VTTTMLWIGGQITTLSIMKSLFLPSLINLLVPLTVTAFILKGKPVIAPARQEDGRGLT
                     ATSFESSLMFFMGMAILVMVPVFKTITHLPPFLGILFGVGILWLVGDLLHSDKTDEDK
                     NHLTIGHALSKIDMSSVVFFIGILLAVASLEHTHILGSLAEWLDKTVGRQDVIVIIIG
                     LVSAVVDNVPLVAASMGMYSLAQYPPDSFLWEFIAYCAGTGGSILIIGSAAGVAAMGL
                     EKINFFWYMKKISGLALIGYFGGAIWYIVEMRLFPH"
     gene            complement(2538..2954)
                     /locus_tag="PLB25_07885"
     CDS             complement(2538..2954)
                     /locus_tag="PLB25_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41531.1"
                     /translation="MNTIRYQTIIGGLGRPRFACLLGLCRHLQKQFPENVLLSFCLFY
                     WVNQMNKSLVLAAVIAAAALVACGKKAEAPAPAPAPVAAPAPAAAASVVEAATSAATA
                     AASATTAVASAADAAGDAAKKAVDAAADAAKKAVSK"
     gene            complement(2987..4129)
                     /locus_tag="PLB25_07890"
     CDS             complement(2987..4129)
                     /locus_tag="PLB25_07890"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOE41532.1"
                     /translation="MDVTQALPLLGGISPQLFMKRHWQKKPLLVRQAVPDFKPLLNRT
                     ELFELAANEDAQTRMVIGQPGKKPAWQFKQGPFPRKALPPLKTPGWTILVQGVDLHVE
                     SVHQLMNQFRFVPDARLDDLMISYATDGGGVGPHFDSYDVFLLQAHGRRRWRIGRQKD
                     LSLEPDVPLKILTHFVPEEEFVLEPGDLLYLPPGYAHDGIAEGECMTYSIGFRIPNRA
                     ELARELLQRVAEDAQDEVGLALYKDPNQVAVEQPAEIPLQLLAFAQKAVQAALTEPHA
                     FARGLGEYMTEPKPNVWFDTHELTESDSQLKGLGVRLDRRSKMLFDDRHIFVNGESFT
                     ASGKDAALMRSLANQRFLPAKDLKKLSAQAFDLVQSWAQAGWLHGF"
     gene            4197..4730
                     /locus_tag="PLB25_07895"
     CDS             4197..4730
                     /locus_tag="PLB25_07895"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOE41533.1"
                     /translation="MKITQQCVAALSWTLMDTLGEELDVLDEPVEFLVGGDDLFQVIE
                     DALQGCSAGSTVNLHIEPEQGFGEYDEQRVFLEPRRLFPDELEEGMIFEGSALPSGCS
                     PDTPQDAIYTVTDVYPEHVVLDGNHPLAGIALLLTLRVRAVREATEAEIGSGTAGTGF
                     FKLAPGHDLPAAKQTLH"
     gene            complement(4776..5225)
                     /gene="rnhA"
                     /locus_tag="PLB25_07900"
     CDS             complement(4776..5225)
                     /gene="rnhA"
                     /locus_tag="PLB25_07900"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HOE41534.1"
                     /translation="MNAIEIYTDGACKGNPGPGGWGALLTSAGSQKELFGGEMGTTNN
                     RMEMMAVIEALSALKRPCQVTLHVDSQYVLKGMTEWLPGWKARGWKTAAKEPVKNVDL
                     WQRLDALVNGGQHQIEWRWVRGHNGDAGNERADQLANRGVELARQQR"
     gene            complement(5250..6053)
                     /locus_tag="PLB25_07905"
     CDS             complement(5250..6053)
                     /locus_tag="PLB25_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOE41535.1"
                     /translation="MSDQIIGLHQWLASPPGQYLLDWECAQMAETVANLFGYHALQLG
                     LPELDALASNRMPHRWLVTQSSLSADLSQLGAGPRAALVSDFAALPFPANSLDLVILP
                     HALELNPDPHGVLREVARVLVPDGRVVICGLNPLSLWGFRQSRAHLYRRFGLGRRFLP
                     EAGEFIGYLRLRDWLSLLNLEIESGHFGCWRPAFSSQAWLQRFGWMDAVGGRWWPILG
                     AVYFIVAVKRVRGMTLLSPGWKVSRRLGNAPVSVANSAGSTRATGQNDL"
     gene            6052..6819
                     /gene="gloB"
                     /locus_tag="PLB25_07910"
     CDS             6052..6819
                     /gene="gloB"
                     /locus_tag="PLB25_07910"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HOE41536.1"
                     /translation="MKLIPLPAFDDNYIWMLHDGQRALVVDPGDPEVVLDALQRQGLN
                     LAAILVTHHHGDHTGGVSVLRQATGAEVFGPKSERMPEPITRLQDGDVIQALDLSCEV
                     LDVPGHTAGHIAYLLQPAGQAAILFCGDTLFSAGSGRLFEGTPAQMLNSLTRLAALAD
                     DTRVCCAHEYTASGLVFARAVEPDNQALVSYQLRVRALRLQQLPTLPSTIGLEKAINP
                     FLRTHIDSVAASIRQFDPNAHNPVSVFAALRTWKNQF"
     gene            6849..>8323
                     /locus_tag="PLB25_07915"
     CDS             6849..>8323
                     /locus_tag="PLB25_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HOE41537.1"
                     /translation="MTLWLSGCASPGARGSDERPYLIGSESDGSAPAWSGDPNRPGLR
                     PPVYPLGGLRPLPAMRLDAQAVHPLTPPVDIWDRIRRGFAMPDLEGELVRDREQWYSS
                     HPDYFARMTERGRKYLFHVVEELEQRNMPTELALLPFIESAFNPQAVSSAHATGMWQF
                     MPATGRTFELKQNMFRDDRRDVLASTRAALDYLQKLYGMFGDWHLALAAYNWGEGSVG
                     RAIAKNQKAGLGIRYTELKMPMETQFYVPKLQAVKNIVASPAAMHVKLPLIENHPFFQ
                     TVDIKRDMDVTVAAKLAEVSLEDFRALNPSASKPVLLAAGTSQILLPWDNAARFQSNL
                     EAYTGGRLASWTAWVCPTTQKVSDAAQRVGMDEAELRAVNQIPPRMMVRAGSTLMVRR
                     TNGIDTDVSNHVADNGQLSLAPELVLRKLQVTAGKKASVASLALRYGVSVAALAEWNK
                     VAASAAFKPGQKVTLFLPGKAVQSARAEPGNTAPPAKSLVAA"
BASE COUNT         1773 a         2519 c         2418 g         1613 t
ORIGIN      
        1 cgctgtcacc caatgggtga actccatcga agctgaaaaa gtcaatattc atgaggactt
       61 acagatgaaa acaagcggtc aactgaggaa tctaggttca agggtgtcag cgctttgcca
      121 tgcaccagat aaccaatcag gttgtcggct gccaggttgg ccatcgccag ccgggtggcc
      181 aaagtggcgc tggcgatgtg cggggtcagc accacgttgg ccaaagccag caagtccgga
      241 tgcactgcgg gctcgccctc aaacacatca agcccggctg ccgcgagctg gccctgccgc
      301 aaggctgctg ccagcgccgc gtcatccaca atgccgccgc gtgcaatatt gaccagcgtg
      361 gcggttggct tcatcagcgc cagctcaggc gcgccaatcg cgtgatgcgc ctgctggctg
      421 tagggcaata ccagcatcag atgatcacag gtgctgagca attcattctt gctcacccag
      481 cgcgcggcac aggcggcttc ggtggcagcg tcgagcctgc tgcggttgtg gtaaatcacc
      541 ttcatgccaa agccgtgggc ggcgcgccgg gcaatgcccc gcccaattcg gcccatgccc
      601 agaatgccca gcgtgctgcc atgcacttca gccccggcga acatgtcata gcgccagcgg
      661 ctccacaagc cagcacgcaa aaaatgctcg gcttctgcca tgcgccgggc ggtcgccagc
      721 agcagggcaa agccaaagtc ggcggtggtg tcagtcagca cgtccggggt gttgctggcc
      781 agcacaccgg ctgcggtcat cgcgtccagg tcaaagttgt tgaaacccac ggccatgttg
      841 gcgcagattt tcaggcccgg acaggccacc agcagcgctg catcaatgcg ttcgctgccg
      901 gtggtcagta cgccttgctt gccctgcagt tggctgatca attgcgcctg cgtccagact
      961 tcatcgagtt ggttggacct gacttcgaag tgctcggcga ggttcgccag aacttgcgga
     1021 aaaaccgcac gcgccaccag aacagcaggc ttcatcgagg ttcaatgagg gaacaggcgc
     1081 atttccacga tataccagat ggcaccgcca aaatagccga tcagggccag gccgctgatc
     1141 ttcttcatgt accagaagaa gttgattttt tccagcccca tggcggcgac accggccgct
     1201 gagccaatga tcagaatcga gccgccagtg ccagcgcaat aggcgataaa ttcccacaag
     1261 aagctgtcgg gcgggtattg cgccaggctg tacatgccca ttgaggccgc caccagtggc
     1321 acgttgtcca ccaccgcgct gaccaggcca atgatgatga cgatgacatc ctggcggcca
     1381 acggttttgt ccagccactc ggccaatgaa cccagaatgt gggtgtgctc cagcgaagcc
     1441 acggccagca gaatgccgat gaagaaaacc accgaactca tgtcgatttt ggacagcgcg
     1501 tgcccaatcg tcaggtggtt tttgtcctca tcggttttgt cgctgtgcag caggtcgccg
     1561 accagccaca aaattcccac accaaacagg atgcccagaa acggcggcag gtgcgtgatg
     1621 gtcttgaaca ccggcaccat caccagaatc gccatgccca tgaaaaacat caggctgctc
     1681 tcgaaactgg tagcggtcag gccgcgacca tcttcctgac gcgccggggc gatcaccggc
     1741 ttgcccttca aaatgaaagc cgtcactgtc aatggcacca gcaggttgat cagcgaaggc
     1801 aaaaacagcg acttcatgat ggacagcgtg gtgatctggc caccaatcca cagcatggtg
     1861 gtggtcacgt caccaatcgg ggtccaggcc ccgccagcat tggctgcgat gacaatgatg
     1921 ccggcaaaaa acagccggtc ttcacgctgg tcaagcagct ttttcatcag cgacaccatg
     1981 acgatggtgg tggtcaggtt gtccagaatg gaactcagga agaaggccac aaagccgacc
     2041 atccacatca gggtagacag cttggtggtc ttgatgcgtg aggtgatcac aacaaaaccg
     2101 ttgtgggcat cgaccacctc gacaatcgtc atcgccccca tcaaaaagaa cacgatttgc
     2161 gcggtgccca tcagcgaggc acccagatgc tcttcgagca gtttgacatc cgaccccatc
     2221 atggcgtaga tggtccaaag cagcccggca ccaatcaggg ccgaggctga tttgttgatt
     2281 tttatttggt gctctaacgc aatcgcggca tacgcgagca caaagacaat ggacagtatt
     2341 gctagcatgt gggttcacct tcaatctttg ggtaacaaat caaaaccctt gattatggcg
     2401 gctgcctgtc gcgccagacc aacaccctgg gtgctgcctg tgtttgtggt caggggcgat
     2461 cgcagcgagc cgcctcctgc gcagcaataa aaaaagccac ccgaaggtgg ctttttttga
     2521 gcaggtcagc ggtccaatta tttggaaacg gccttcttgg cggcatcagc agcagcgtca
     2581 acggccttct tggcagcatc gccagcggca tcagcagcag aagcaacagc ggtggtggcc
     2641 gatgcagcag cggtggcagc agaagtagcg gcttcaacga cagatgcggc agcggcaggt
     2701 gcaggtgcag caacaggtgc cggggcaggt gcaggagctt ctgctttctt gccacaagca
     2761 accagagcag cagcagcgat aacagcggcc aaaacgagag atttattcat ttgatttacc
     2821 caataaaaaa gacaaaagga caaaaggaca ttttccggaa attgtttttg cagatgcctg
     2881 caaagaccaa gcaaacatgc aaaacgcggt ctgcccagac ccccgattat agtttggtat
     2941 ctgatggtat tcacgcttga cgcggcccaa gcgttgtgct tacgcgtcaa aacccgtgca
     3001 gccaacccgc ttgcgcccag ctttgcacca ggtcaaaagc ctgtgcgctg agctttttga
     3061 ggtctttggc cggcagaaaa cgctggtttg ccaaactgcg catcagcgcg gcatctttgc
     3121 cggaagcggt gaaactctcg ccgttgacaa agatatgccg gtcatcaaac agcatcttgc
     3181 tgcgccggtc aagacgcacc cccagcccct tcagttgact gtctgattca gtcagttcgt
     3241 gggtgtcaaa ccagacattt ggcttgggct cggtcatgta ttcgcccagc ccacgggcaa
     3301 acgcgtgcgg ctcggtcaag gcagcttgca ccgccttttg ggcaaatgcc agcagttgca
     3361 acggtatctc ggcaggctgc tccaccgcca cctggttcgg atccttgtaa agtgccagac
     3421 cgacctcgtc ctgcgcgtct tcggccacgc gctgcaacag ttcacgcgcg agttcggccc
     3481 ggttgggaat gcgaaagccg atcgaatagg tcatgcactc gccctcggca atgccgtcat
     3541 gggcatagcc tggcggtaaa taaagcaggt cgcctggctc cagcacaaat tcttcttccg
     3601 gcacgaagtg ggtcaaaatc ttcagcggca catcgggctc caggctcagg tccttttgcc
     3661 ggccaatgcg ccagcgtcgg cgcccatgcg cctgcagcaa gaacacgtca taactgtcaa
     3721 agtgcggccc gacccccccg ccatcggtgg cataactgat catcaggtca tccagccggg
     3781 catcaggcac aaagcggaac tggttcatca actggtgtac cgattcaaca tgcaggtcaa
     3841 ctccttgcac cagaatcgtc cagcccggtg ttttcaaagg cggcagggcc ttgcgcggaa
     3901 acgggccctg tttgaattgc caggctggct ttttgccggg ctgcccaatc accatgcggg
     3961 tttgggcatc ttcgttggcg gccagttcaa acagttcggt gcggttgagc aagggcttga
     4021 aatcgggcac cgcctggcgc accaacagcg gcttcttttg ccaatgccgt ttcatgaaaa
     4081 gctgcgggct gatgccgccg agcaggggca gcgcttgtgt cacatccatt gatttctcca
     4141 aatcgtttga aaactgcggg gaacccgggc cttttcaaac tgcgacaatt ctaggtatga
     4201 aaatcactca acaatgcgtg gctgctttaa gctggacgct catggacact ctgggcgaag
     4261 aacttgacgt gctcgacgag ccggtagagt ttcttgtcgg tggcgatgat ttgttccagg
     4321 tcattgaaga cgcgctgcaa ggctgcagcg ccggcagtac cgtcaacctg cacatcgaac
     4381 ccgagcaggg ttttggtgaa tacgacgaac aaagggtgtt tctggagccg cgtaggcttt
     4441 ttccggatga acttgaagaa ggcatgattt ttgagggtag cgcgctgcct tcgggctgca
     4501 gcccggatac accgcaggat gccatctaca ccgtgaccga cgtttacccg gagcatgtgg
     4561 tgctcgacgg caaccacccg ttagcaggca tcgccctgct gttgaccctg cgggtgcgcg
     4621 cggtgcgcga ggcaaccgag gcagaaattg gcagcggcac cgccggaacc gggtttttca
     4681 agctggcacc ggggcacgac ctgccagcgg cgaagcaaac cttgcactga ggctgtaccc
     4741 ggcgcactgc cctggggcag gcagcgcctg atcggtcaac gctgctggcg tgccagctca
     4801 acgccccgat tggccaactg gtcggcacgc tcgttgccag cgtcgccgtt atggccacgt
     4861 acccagcgcc actcaatctg gtgctggccg ccgttcacca gcgcatcaag gcgctgccac
     4921 aggtcaacgt ttttcaccgg ttccttggct gcggtcttcc agccgcgtgc tttccagccc
     4981 ggcaaccatt cggtcatgcc cttgagcaca tactggctgt ccacatgcag cgtcacctgg
     5041 cagggccgct tcagcgctga caaggcctcg atcaccgcca tcatttccat gcggttgttg
     5101 gtggtgccca tttcgccgcc gaacagttct ttttggctac cggccgatgt caataatgca
     5161 ccccagccac cggggccggg gttgcccttg caggcaccgt cggtgtaaat ttcaatggca
     5221 ttcacgcaat atgtcctcaa gacttgtttt catagatcat tttggcctgt agcccttgta
     5281 ctacctgcgc tgttagctac agaaacggga gcatttccaa gccgcctgga gaccttccag
     5341 ccggggctga gcaaggtcat gccacgcacc cgcttgaccg ccacaataaa gtagacagcc
     5401 cccagtatcg gccaccagcg gccacccacc gcgtccatcc agccaaagcg ctgcaaccag
     5461 gcctgactgg aaaaggccgg tcgccagcag ccaaaatgcc cgctctcgat ttccagattg
     5521 agcaaactga gccagtcacg caagcgcagg tagccgatga attcgccagc ctcgggcaaa
     5581 aaccggcgac ccagtccaaa acggcggtac agatgcgccc ggctctggcg aaacccccaa
     5641 aggctcaagg ggttgaggcc gcaaatcacc acccgcccat cgggcaccag cacccgcgct
     5701 acttcgcgca gcacaccatg cgggtcgggg ttgagttcca gcgcatgggg caatatcacc
     5761 agatcaaggc tgttggccgg aaacggcagg gctgcaaagt cgctcaccag cgcagcgcgc
     5821 gggcctgcgc ccagttgcga cagatcggcc gacaagctgc tctgggtgac cagccagcgg
     5881 tgcggcatgc ggttggacgc caacgcgtcg agttcgggca aacccagttg caaagcatgg
     5941 tagccaaaca ggttggccac cgtctcggcc atctgcgcgc actcccaatc cagcaggtac
     6001 tgccccggtg ggctggccag ccactggtgc aaacctataa tttgatcgct catgaagtta
     6061 attccactac ctgctttcga cgacaactac atctggatgc tgcacgacgg gcagcgcgcc
     6121 ctggtggttg accccggcga ccctgaggtg gttctcgatg cgctgcaacg ccaggggctg
     6181 aatctggcgg cgattttagt cacgcaccac catggcgacc acactggcgg ggtaagtgtg
     6241 ctgcgccagg ctaccggcgc cgaggtgttt ggccccaaaa gcgaacgcat gccggagccc
     6301 attacccggt tgcaggacgg cgatgtgatc caggcactgg acttgtcgtg tgaggtgctg
     6361 gatgtgcctg gtcatactgc cgggcacatt gcctatctgc tgcagcctgc cgggcaggcc
     6421 gctatattgt tttgtggtga cacgctgttc agcgccggca gcgggcgcct gtttgagggc
     6481 accccggccc agatgctgaa ctccttgacc cggctggcag cgcttgcaga cgacacccgg
     6541 gtctgctgtg cacacgagta caccgccagc ggtctggttt ttgcgcgcgc cgttgagccc
     6601 gacaaccagg ccttggtttc ttaccagtta cgggtgcggg cgctgcggtt gcagcagttg
     6661 ccaacgctgc cgtccacgat tgggcttgaa aaggccatca atcctttttt gcgcacgcac
     6721 atcgatagcg tcgctgcatc aatccgccag tttgacccga acgcgcacaa cccggtgagc
     6781 gtttttgcgg ccctgcgaac ttggaaaaac cagttttgaa aaatttgtcc gttcttctgt
     6841 tgttgctgat gaccctgtgg ctgtcgggct gtgccagccc cggtgctcgg ggcagtgacg
     6901 aaaggcccta tctgattggc agcgagtctg acggcagcgc gcccgcttgg tcgggtgacc
     6961 ccaatcggcc gggcctgagg ccgccggttt acccgctcgg cggtttgcgc ccattgcctg
     7021 cgatgcggct ggatgcgcag gcggtgcacc cgctgacgcc accggttgac atttgggacc
     7081 gcatccggcg cggctttgcc atgcccgacc tcgaaggcga actggtgcgc gaccgcgagc
     7141 agtggtacag cagccaccca gattactttg cacgcatgac cgagcgcggc agaaagtacc
     7201 tgttccatgt ggtcgaagaa ctggaacagc gcaacatgcc aaccgaactg gcgctgctgc
     7261 cgttcatcga gagcgccttc aacccgcagg ccgtatcaag cgcccatgcc accggcatgt
     7321 ggcagttcat gccagccacc ggcaggactt tcgagctcaa gcaaaacatg tttcgcgatg
     7381 accggcgcga cgtgctggcc tcgaccaggg cagcgctgga ctacctgcaa aagctctatg
     7441 gcatgtttgg cgactggcat ctggcgctgg ccgcctacaa ctggggcgaa ggcagcgttg
     7501 gccgggccat tgccaagaac cagaaagccg gattgggcat tcgctacacc gagctgaaaa
     7561 tgccaatgga aacccagttt tatgtgccca aactgcaggc agtcaagaac attgtggcaa
     7621 gtcctgcggc gatgcatgtc aagttgccgt tgatcgagaa ccaccctttt ttccagaccg
     7681 tcgatatcaa gcgtgacatg gatgtgacgg tagccgccaa actcgccgaa gtgtcgctgg
     7741 aggactttcg ggcattgaac ccatcagcct ccaaacctgt actgctggcc gcaggcacct
     7801 cccaaatcct gttgccatgg gataacgccg cgcgatttca gagcaacctg gaggcctata
     7861 ccggtggccg actggccagt tggaccgcct gggtttgccc caccacgcag aaggtttcag
     7921 acgcagcaca acgggttggc atggacgagg ccgaactgcg tgcggtcaac cagattccgc
     7981 cgcgcatgat ggtgcgggct ggctcgaccc tgatggtcag gcgcaccaac ggcatcgata
     8041 ccgatgtcag caaccatgtg gccgacaatg gccagttgtc gctggcacct gagctggtgc
     8101 tgcgcaagtt gcaggtgaca gccggcaaaa aagcatccgt tgcctcgctg gcgctgcgtt
     8161 acggcgtcag tgttgcagcg ctcgccgaat ggaacaaggt ggctgcatct gctgccttca
     8221 agcccggaca aaaagtcacc ctgttcctgc caggcaaggc ggtgcagtcg gcaagggccg
     8281 agccgggcaa tactgcaccg ccagctaaat cgctggtggc ggc
//
LOCUS       DAOSLL010000137         8307 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8025_length_8307_cov_4.789627, whole genome shotgun sequence.
ACCESSION   DAOSLL010000137 DAOSLL010000000
VERSION     DAOSLL010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8307
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8025_length_8307_cov_4.789627"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(123..410)
                     /locus_tag="PLB25_07920"
     CDS             complement(123..410)
                     /locus_tag="PLB25_07920"
                     /inference="COORDINATES: protein motif:HMM:NF016167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HOE41538.1"
                     /translation="MATVKSNALPPSLSSWCSNINSDGEFGALNEQNHTQTRHAGRQT
                     FFRGLPITVYDVLSMLSSGMTQEEVLTDFPELMAEDILSVLAYAADREHQV"
     gene            complement(439..3420)
                     /gene="gcvP"
                     /locus_tag="PLB25_07925"
     CDS             complement(439..3420)
                     /gene="gcvP"
                     /locus_tag="PLB25_07925"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HOE41539.1"
                     /translation="MTMPHTPALADLENPSEFIARHIGIDAAEEALMLQAVGAPSRQA
                     LMDEIVPPSIVRSRPMDIPSAITEAAALAELKAIAAKNQVFKSFIGQGYYGTHTPGVI
                     LRNILENPAWYTAYTPYQAEISQGRMEALVNFQTMVTDLTALPMANASMLDEATAAAE
                     AMTLALRAGKSKSSTFFVDDEVLPQTLEVIRTRAEPLGISVKLCQGDEALAQDSFAVL
                     LQYPGVSGVVRDDRDWIKAYKARGGLVIMAADLLALTLLTPPGELGADIAIGSSQRFG
                     MPMGNGGPHAAYLACRDEFKRAMPGRLVGVSVDAYGNPAYRLALQTREQHIRREKATS
                     NICTAQVLPAVMASMYAVYHGPQGLQRIARRVARLTAVLAAGLQQMGYTLHHGSAFDT
                     LTVNTEEATNKIAANATAATANLRFASKTTLSISLDETSTREDIALLWSCFAKDGQSL
                     PQLAALQDRVSDLLPAALLRSSAYLSHPVFNSHHSETSMLRYIRRLSDFDLALDRSMI
                     PLGSCTMKLNATSEMLPITWPEFADIHPFAPAEQRRGYQLLDDQLSAWLCQATGYAGV
                     SLQPNAGSQGEYAGMLAIRAFHAARGEAHRNICLIPSSAHGTNPASATMVGFKVVVTA
                     CDQQGNVDMADLKAKCELNSANLAAIMITYPSTHGVFETSVKELCALVHSHGGRVYVD
                     GANMNALVGLAAPGEFGGDVSHLNLHKTFCIPHGGGGPGVGPVCVVADLLPFLPGHVT
                     ALLASPAPLAAGGAVATPDALGLPAQEHRNVGCAVGAISGAPLGNAAVLPISWMYCRM
                     MGADGLKAATEVAILSANYISARLSGHYPTLYTSAPVDGKPGRVAHECILDLRPLKDS
                     SGGTLGISAEDVTKRLMDYGFHAPTLSFPVPGTLMVEPTESETLDELDRFINAMIAIR
                     AEIARVERGEWPQDNNPLKNAPHTAAALLGEVWDRPYPRALAAFPVASLKHSKYWPPV
                     GRIDNVYGDRHLFCSCVPVGE"
     gene            complement(3754..4128)
                     /gene="gcvH"
                     /locus_tag="PLB25_07930"
     CDS             complement(3754..4128)
                     /gene="gcvH"
                     /locus_tag="PLB25_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HOE41540.1"
                     /translation="MSIKYTPDHEWIKIDGNSATVGITHHAQDALGDVVFVDLPEVGK
                     TYAARDAVAVVESVKAAADVYMPANGDITEVNEALRADPSLANSDPLGAGWFFKMLLA
                     NPAELDAMMDETSYTSYAADAH"
     gene            complement(4228..5373)
                     /gene="gcvT"
                     /locus_tag="PLB25_07935"
     CDS             complement(4228..5373)
                     /gene="gcvT"
                     /locus_tag="PLB25_07935"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HOE41541.1"
                     /translation="MSDSPDPLLTVPLNDLHIALGARMVPFAGYAMPVQYPGGLIAEH
                     RHTRTAAGLFDVSHMGQLRLVGPNAAAALETLLPVDVIDLAVDRQRYGLLLNEAGGII
                     DDLMFVNRGSDIFVIVNGACKAADIAHIQASIGGRCEVIPEPGQALLALQGPLAVQAL
                     SRLAPGVEKLVFMTGGHFRVHAGRQDIDVFLTRSGYTGEDGFEISVRDSEALALANAL
                     LAQPEVKPIGLGARNSLRLEAGLPLYGNDIDATTTPVEAGLNWAMQKVRRAGGARAGG
                     FPGATKILAQLAGGTGAASRKRVGLVALERVPVREHTELQDSQGNRLGEVTSGLLGPS
                     IDKPIAMAYVPPEFAALGTKINAMVRGKPVPMQVAAMPFVPTNYFRG"
     regulatory      complement(5447..5586)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      complement(5608..5721)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(5957..6856)
                     /locus_tag="PLB25_07940"
     CDS             complement(5957..6856)
                     /locus_tag="PLB25_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008417 - fucosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 10"
                     /protein_id="HOE41542.1"
                     /translation="MATRCILFFDTMWGSALDRPDGLPEHYQLTADRQCFGTADAVVF
                     HIPQWRYGHGESMPGKPPGQIWVAWSMECEENYPLLRDAAFMRHFDFSMTYRLDSDFP
                     VTYVSQSLDTALRAPGQVSPSQGLVAAFISSPYNRSGRQAYFQELARWLSIDSYGKFL
                     RNRTLPADVGQASKLTTLAGYKFTLAFENACARDYVTEKFFDPLTVGSVPIYLGAPNI
                     ADFAPGEHCYIDVRDFADPRSLARHIEQLGADEAAYRHYLDWRQQPFRSGFQDLLAQA
                     ARHPFVRLCEGIEARLAARASAS"
     gene            complement(7053..8258)
                     /locus_tag="PLB25_07945"
     CDS             complement(7053..8258)
                     /locus_tag="PLB25_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family efflux MFS transporter"
                     /protein_id="HOE41543.1"
                     /translation="MSPHSHPTTMRPGAIVLLLSLLLGLQPITTDLYLPALPVLTVGF
                     AAPMAQAQLTLTALLLAFGGSQLVWGPLSDRYGRRPILLIGMAAYVLAAVASALAPSM
                     LWLIVWRTVQGAAMGAGVMCARAIVRDLYAPAQGARVMSKGLSGLGVIACLSAPLGGL
                     LTDWLNWRFALLALSVFGAATLAVVALRFTETVRQKNPHALQATALLATWRQILRHPT
                     FWAFSALSGASYGGLFTFLAASSFVFTGVLGLSKTQYGLVMFVNSLAYIAGTFVCRAW
                     LSRYGVRRSVALAAVLTLTGGTLMALLTLAGFVSVTAIMLPQMLFMIGHGIHQPCGQS
                     GAVGPFPQAAGAASAVNGFLMMLCAFAMGWWLGAHMDGTVLPLTLGIWFWSALIATSA
                     WTLVQRHGG"
BASE COUNT         1632 a         2520 c         2587 g         1568 t
ORIGIN      
        1 gcggctttcg ccctacccag acgcgccgcc agcggcccac tcgggcgcgt ccaaccgagg
       61 aatcaaggat catctttggc tttttgccat acgtctttag ctgacgcaga tgccatgcca
      121 agtcaaacct gatgctcccg gtctgctgca taggccagca cagacagaat atcttccgcc
      181 atcaattcag gaaaatcagt cagaacttct tcttgcgtca tcccgctcga caacatggat
      241 agaacgtcat ataccgtgat gggcagcccg cgaaaaaagg tctgccgccc cgcatgcctg
      301 gtttgagtgt gattctgttc attcaaggct ccaaattcac catcagaatt gatgttcgag
      361 caccaactcg acaaactcgg cggcaatgca ttggatttca ccgtcgccat gtcgccccca
      421 gacgcttgtt tcctgccact actccccaac cggcacgcaa gagcaaaaca gatggcggtc
      481 gccataaacg ttgtcaatgc gcccaaccgg cggccagtat ttgctgtgct tcaggctggc
      541 caccggaaaa gccgccagcg cacgcgggta cggccggtcc cacacttcac cgagcaatgc
      601 ggcggcggtg tgcggggcat ttttcagcgg gttgttgtcc tgcggccact cgccgcgctc
      661 gacccgggcg atttcggcgc ggatggcgat catggcgttg atgaagcggt cgagttcgtc
      721 gagggtttcg ctctcggtcg gctcaaccat cagcgtgccg ggcaccggaa agctcaaagt
      781 cggcgcatga aagccatagt ccatcagccg cttggtgaca tcttcggcgc tgatgccaag
      841 ggtgccgccg ctgctgtcct tgagtgggcg caaatccaga atgcactcat gtgccacccg
      901 acccggcttg ccgtcaaccg gagcgctggt gtacagcgtg gggtaatggc cagacaagcg
      961 ggcgctgatg tagttggcgc tcagaatcgc gacttcggtg gcggctttga gtccgtctgc
     1021 gcccatcatg cggcagtaca tccaactgat tggcagcacc gctgcattgc ccagcggggc
     1081 gccagatatg gctcccaccg cgcagcccac gttccggtgc tcttgcgcag gcagcccaag
     1141 cgcatcaggc gttgcaactg cacctcccgc tgcaagcggt gctggtgacg ctagcaaggc
     1201 ggtcacatgt cccggcaaaa agggcagcag atctgccacc acgcagaccg gcccgacacc
     1261 ggggccaccg ccgccgtgcg gaatgcagaa tgtcttgtgc aggttcaggt ggctgacatc
     1321 gccaccaaat tcgccggggg cggccaggcc gaccagcgca ttcatgttag cgccatcgac
     1381 atacacccgc ccgccgtggc tgtgcaccag cgcgcacagc tctttcacgc tggtctcgaa
     1441 cacgccatgg gtgcttgggt aggtgatcat gatggcggcc agatttgcgc tgttcagctc
     1501 gcatttggcc ttcaggtcgg ccatatcgac attgccttgt tggtcgcagg cggtcaccac
     1561 caccttgaag ccgaccatgg tggcgctggc cgggttggtg ccgtgcgcgc tgctcggaat
     1621 caggcagata ttgcggtggg cttcgccgcg cgcggcgtga aaggcgcgaa tcgccagcat
     1681 gccggcgtat tcgccctggc tgccggcgtt gggctgcaag ctgacaccgg cataaccggt
     1741 ggcctggcat agccaggccg acagttgatc atcgagcagt tggtagccac ggcgctgttc
     1801 ggccggggca aacgggtgaa tgtcggcaaa ctctggccag gtgatcggca gcatctcgct
     1861 ggtggcgttg agtttcatgg tgcaactgcc cagcggaatc atgctgcggt cgagcgccag
     1921 gtcaaagtcg ctcaggcggc ggatgtagcg cagcatgctg gtctcagaat ggtggctgtt
     1981 gaacaccgga tggctcagat aggcactgct gcgcagcagg gcagcgggca gcaggtcgct
     2041 gacacggtct tgcagcgctg ccagttgcgg caggctctgg ccgtctttgg caaagcatga
     2101 ccaaagcagc gcgatgtcct cgcgcgtgct ggtctcgtca agcgatatgc tcaatgtggt
     2161 ttttgaagca aatcgcaggt tagccgttgc tgcagtagcg ttggcagcta ttttatttgt
     2221 agcttcttcg gtgttcacgg tgagcgtgtc gaaggcgctg ccgtggtgca gggtgtagcc
     2281 catttgctgc aaacctgctg ccagcacggc ggtcaaccgg gccacgcgcc gggcgatgcg
     2341 ctgcaggcct tgcgggccgt gataaacggc gtacatactg gccatcacgg ctggcagcac
     2401 ttgcgccgtg cagatgttgg aagtggcttt ttcacgccgg atgtgctgct cgcgcgtttg
     2461 cagtgccagc cggtaagccg ggttgccata ggcatcgacg cttaccccga ccagacggcc
     2521 cggcatcgca cgtttgaatt catcgcggca cgccaggtaa gcggcgtgcg ggccaccgtt
     2581 gcccatcggc atgccaaagc gctggctgct gccaatggca atgtcggcac cgagttcacc
     2641 gggcggggtc agcagcgtca aggccagcag gtcggcggcc atgatgacca ggccgccccg
     2701 ggctttgtag gccttgatcc agtcccggtc gtcacgcacc acgccgctga caccggggta
     2761 ttgcagcagc acggcaaagc tgtcttgtgc cagtgcctca tcgccctggc aaagtttgac
     2821 gctgatgccc agcggctcgg cgcgggttct gatcacctcc agcgtttgtg gcaacacctc
     2881 gtcatcgaca aaaaaggtgc tggacttgga cttgcctgca cgcagcgcca gtgtcatggc
     2941 ttcggccgct gccgtggctt cgtcgagcat cgacgcattg gccattggca aggcggtcag
     3001 gtcggtgacc atggtctgaa agttgaccag ggcttccatc cggccctggc ttatttcggc
     3061 ctggtacggc gtgtaggcgg tgtaccaggc cgggttttcc aggatgttgc gcaggatcac
     3121 gcctggcgtg tgggtgccgt aatagccctg gccgatgaag cttttgaaca cctggttttt
     3181 tgccgcaatg gccttgagct cggccagcgc agcggcctcg gtgatggccg acggaatgtc
     3241 catcgggcgg ctgcgcacaa tcgacggcgg cacgatctca tccatcaacg cctggcgcga
     3301 cggcgcgccg acggcttgca gcatgagggc ttcttcagcc gcgtcgatgc cgatgtggcg
     3361 ggcgatgaat tccgacgggt tttccaggtc ggcaagcgct ggggtgtggg gcatggtcat
     3421 ggcatttcaa tcaggcgttt gcccggggcc tgtcgcaagg cgggcgaaac aatcaatgga
     3481 accgggcttc gacaggccct gcccgaaccg gtgctggcag atcaggtgct ctgggagcct
     3541 gtcggactta tggaacatcg gctgcaaatc gaccgcagcg gcccattttg caccggcttt
     3601 ttgacccata gcggggctat gggcctgcaa atccggcgca aactgtgctc gctggggtcg
     3661 attttcgctg ttcgatccat aagtccgaca ggctcctagg catgaacacg ctggcagcgc
     3721 atccggttgt caagctggcg gcacagcggt ttgtcagtgc gcgtcagcgg cgtagctggt
     3781 gtagctggtc tcatccatca tggcatcgag ctcagccggg ttggcaagca gcatcttgaa
     3841 gaaccagccc gcaccgagcg ggtcggaatt ggccagcgac gggtcggcgc gcagggcctc
     3901 gttgacctcg gtgatgtcgc cgttggcggg catgtacaca tctgctgccg ccttgaccga
     3961 ctcgaccacc gcaacggcat cgcgggcggc ataggtcttg ccaacttcgg gcaaatcgac
     4021 aaacaccacg tcgcccaaag cgtcctgcgc gtggtgcgtg atgccgacgg tggcgctgtt
     4081 gccgtcgatc ttgatccatt cgtggtcggg ggtgtacttg atgctcatgg gggtctccag
     4141 gaaaaacgat gggggtgaaa gtgggcgtga tggcatgaaa cgggtcattg ccacgcgggt
     4201 acgcatggcg cagttgattg ccgcagccta gccgcggaaa tagttggttg gcacaaacgg
     4261 catggcggca acctgcatcg gcaccggctt gccgcgcacc atggcattga tttttgtacc
     4321 cagtgcggca aattcgggcg gtacataagc catggcgatg ggcttgtcga tgctcgggcc
     4381 cagcaagccg ctggtgactt cgcccagccg gttgccctgg ctgtcctgca actcggtgtg
     4441 ttcacgtacc ggaacccgct ccagcgccac cagtccaaca cgttttcggc ttgcagcgcc
     4501 cgtcccgcct gccagttgag ctagtatttt agtagcgcct ggaaaaccgc cagcgcgggc
     4561 gccgcctgcg cggcggacct tttgcatggc ccagttcagg cccgcctcga ccggtgtggt
     4621 ggtggcatca atgtcattgc cgtacagcgg caggccagct tcaagccgca acgagttgcg
     4681 cgcacccagg ccaatcggct tgacctcggg ttgcgccagc agtgcattgg ccagtgccag
     4741 cgcctcagag tcgcgcaccg atatctcaaa gccgtcttcg ccggtgtagc cgctgcgggt
     4801 cagaaacacg tcaatgtcct ggcgaccggc atgcaccctg aagtggccac cggtcatgaa
     4861 caccagcttt tccacgccgg gtgccaaacg tgacagggct tggaccgcca gcgggccttg
     4921 caatgccagc aaggcttgcc caggctcggg gatcacctcg cagcgccctc cgatgctggc
     4981 ctggatatgc gcgatgtcag cggccttgca ggcaccgttg acgatgacaa aaatgtcgct
     5041 gccccggttg acaaacatca ggtcgtcgat gatgccgcca gcttcgttga gcaacaggcc
     5101 gtagcgctgt cggtcaacgg ccaggtcaat cacatcaacc ggcagcaggg tttccagtgc
     5161 ggcagcggca ttggggccga ccaggcgcaa ctgccccatg tgcgacacgt caaaaaggcc
     5221 agccgccgtg cgggtgtgcc tgtgttcggc aatcaggcca cccgggtatt gcaccggcat
     5281 ggcataaccg gcaaacggca ccatgcgcgc accgagtgcg atgtgcaagt cgttcagcgg
     5341 cacggtcaaa agtgggtcag gtgagtcaga catcagggca ctccagatca agcgaatcaa
     5401 agggcaaatc aaacgccatg gcgcaaacgc atcgtccatg ccatggcctg cccccgctgt
     5461 ccgctttacc tgagagattc gccgggccgg gccacttgaa acagcggcac aggccaacgg
     5521 gttgctcctt cggtgggtga caccgcgctg tgtggcgggg tgctacctct ctccagtggg
     5581 gatacgtcag aagtcgggcg acttcaaaga cgcttgtcag tccttttgcc tgagcgttca
     5641 aactgctgca atcagcaact ctgcgccttc ggcggctgcc ttgaagtggc ttcaaaggca
     5701 acgctctcct gaccgggcaa attctacatg cttgagtttg cgcggctgaa aaacgctgcc
     5761 tttcggtggc aggctgttcc atccatcagc acagcaactt caactgcatt gccaacggtc
     5821 tgtgcgcgtg tgagtgacca gaggtccgca tagcccagtc atttggccgg gaattggaca
     5881 tggcccccac ggtcgatgcc ttggcagcgg cgcactgcaa gcggcgacct gcagtgttgc
     5941 caggtcggtc agggcctcaa ctggctgacg cgcgcgccgc caggcgagct tctatgccct
     6001 cgcacaggcg cacgaacggg tgcctggcag cctgtgccag caaatcctga aagccacttc
     6061 ggaaaggttg ctgtctccag tccaggtagt ggcgataggc cgcttcatcg gcacccaact
     6121 gctcgatatg gcgggcgaga gagcggggat cggcaaaatc acgaacgtcg atatagcagt
     6181 gctctccagg tgcgaaatcc gcgatgttcg gcgcacccag gtagattggc actgagccga
     6241 cggtcagcgg gtcaaaaaac ttctcggtga cgtaatccct ggcacaggcg ttttcaaagg
     6301 ccagcgtgaa cttgtagcca gccagggtgg tcagcttgct tgcctgcccg acatctgctg
     6361 gcaaagtgcg gttgcgcaaa aatttgccgt aagagtcaat cgatagccag cgtgccaatt
     6421 cctggaaata tgcttgccgg ccgctgcggt tgtaaggact ggaaataaat gcagcgacca
     6481 ggccttgcga tggactgacc tgcccaggcg ctcgcaaggc ggtatcaagt gattgactga
     6541 cataggtcac cgggaaatcg gagtccaacc gataggtcat gctgaaatcg aaatggcgca
     6601 tgaacgctgc atcgcgcagc aatgggtagt tttcttcgca ttccatcgac caggcgaccc
     6661 agatctgccc aggcggcttg ccgggcattg attcgccatg accgtaacgc cattgcggaa
     6721 tgtggaaaac gacagcatcc gctgtgccga agcactggcg atctgccgtt agttggtagt
     6781 gctcaggcag cccatctggc ctatcgagtg ccgagcccca catcgtgtcg aagaaaagta
     6841 tgcagcgtgt agccatgttg cccttttcaa atttgacggg caatctgcat tgacggggaa
     6901 agactgcccg ccaaagccag atagttgccc caaattgctg atggtaaata aatttctcaa
     6961 aatcagttga agcctggtca tgacctcaag tggaaccctt tttggtgccg actttgccat
     7021 gtcaatgctg cgctgttgcc ccaaagcgcc gttcaacccc catgccgttg caccagggtc
     7081 caggccgatg tggcgatcag cgcgctccag aaccagatac ccagggtcag cggcagcacc
     7141 gtgccgtcca tgtgggcacc cagccaccag cccatggcaa atgcgcagag catcatcaag
     7201 aagccattca ccgcagaggc cgccccggcg gcttgcggaa acggcccgac tgcaccactc
     7261 tggccgcagg gctggtggat gccatggccg atcatgaaca gcatttgtgg cagcatgatg
     7321 gcggtcacgc tgacaaaacc ggccagcgtc agcagcgcca tcagcgtgcc gccggtgagg
     7381 gtcaggacag cggccagcgc cacgctgcgg cgcaccccgt agcgtgacag ccaggcccgg
     7441 cagacaaagg tgccagcgat ataggccagc gaattgacga acatcaccag cccatactgg
     7501 gttttgctca ggcccagcac gccggtgaag acaaatgatg atgctgccag aaaggtaaac
     7561 agcccaccat acgaggcgcc cgacagggct gaaaaggccc aaaacgtcgg atgccgcagt
     7621 atctggcgcc aggttgccag caaggctgtt gcctgcagcg catgcgggtt tttttgccgc
     7681 acggtttcag taaagcgcaa cgccacgacg gccagtgtgg cagcaccaaa gacgctcagc
     7741 gccagcaagg caaagcgcca gttcagccag tcggtcagca agccacccag gggcgcgctc
     7801 aggcaggcaa tcacgccgag cccgctcagg cccttgctca tgacacgcgc gccctgcgcc
     7861 ggggcataca agtcacgcac gatggcgcgc gcacacatca ccccggcacc catcgccgca
     7921 ccctgcacag tgcgccagac gatcagccag agcatgcttg gtgccagcgc gctggccacc
     7981 gccgccagca cataggcggc catgccgatc agcaagattg gccgacggcc atagcggtca
     8041 gacagcggcc cccagaccaa ttgcgagccg ccaaaggcca gcagcagggc ggtcagagtc
     8101 agttgcgcct gcgccattgg cgcggcaaag ccgacggtca gtaccggcag ggcgggcaaa
     8161 tacagatcgg tggtgatcgg ctgcaggccc agcagcaaag acagcaacaa gacgattgcg
     8221 ccaggacgca tggtggttgg gtgcgagtgg gggctcattg gtttgagcgt agcagattcg
     8281 ccctgcacag acgccacagt aaagggt
//
LOCUS       DAOSLL010000138         8306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8028_length_8306_cov_5.166768, whole genome shotgun sequence.
ACCESSION   DAOSLL010000138 DAOSLL010000000
VERSION     DAOSLL010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8306
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8028_length_8306_cov_5.166768"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="PLB25_07950"
     CDS             complement(<1..710)
                     /locus_tag="PLB25_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Orn/Lys/Arg decarboxylase N-terminal
                     domain-containing protein"
                     /protein_id="HOE41544.1"
                     /translation="MKFRFPIVIIDEDFRSENTSGLGIRALAHAIESEGFEVLGVTSY
                     GDLSQFAQQQSRASAFILSIDDEEFTPGPDLDPAVLNLRHFIEEVRRKNLDVPIYIYG
                     ETKTSRHLPNDILRELHGFIHMFEDTPEFVARHIIREAKSYLEGVQPPFFKALLDYAE
                     DGSYSWHCPGHSGGVAFLKSPVGQMYHQFYGENMLRADVCNAVEELGQLLDHDGAIGK
                     SERNAARIFNADHCFFVT"
     gene            840..1274
                     /locus_tag="PLB25_07955"
     CDS             840..1274
                     /locus_tag="PLB25_07955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HOE41545.1"
                     /translation="MADSTLWWLAAGALIAAELITGTFYLLMLSTGLAAAALVAHAGL
                     GMPWQWASAAAVGCGSVLAWRSLKQSRPSNAPAGANHDVNMDIGGTVQVAAWREDGTC
                     SVKYRGAQWDAALQAGEPAQAGSHVIVEVIGSRLILKKQPNS"
     gene            1290..2201
                     /locus_tag="PLB25_07960"
     CDS             1290..2201
                     /locus_tag="PLB25_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HOE41546.1"
                     /translation="MDILAIVLFVIAVIFITQSIKVVPQQHAWVVERLGKYNGTLTPG
                     LSFLMPFIDKVAYKHLLKEVPLDIASQVCITRDNTQLQVDGILYFQVTDAMRASYGSS
                     NYIVAISQLAQTSLRSVIGKLELDKTFEERDIINAQVVQAIDEAALNWGVKVLRYEIK
                     DLTPPKEILHAMQQQITAEREKRALIAASEGRRQEQINIATGEREAFIARSEGEKQAV
                     INKAQGEAASILAVAEATGQAIERVAAAIRQPGGEQAVQLKVAEKAVEAYGRVASDAT
                     TTLIIPGNMTEVSALISSAMKMIQTQK"
     gene            complement(2687..3403)
                     /gene="kdpE"
                     /locus_tag="PLB25_07965"
     CDS             complement(2687..3403)
                     /gene="kdpE"
                     /locus_tag="PLB25_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HOE41547.1"
                     /translation="MSAPVAILIEDEPQIRRFVRAALESEGWLVFEAENVRTGLQHAA
                     TRKPDLLVLDLGLPDGDGLDLIREVRGWSNVPIMVLSARTDEGDKIAALDAGADDYLT
                     KPFGIGELLARVRANLRRPRSTAGGQMDARSGELDPLFRFGEVEVDRQARIVRRNHTA
                     IHLTPIEYRLLTVLMANAGRVMTHRQLMRDVWGPAHADQSHYLRIYMGHLRQKLEVDP
                     AQPQHLLTETAVGYRLLPDS"
     gene            complement(3410..6175)
                     /locus_tag="PLB25_07970"
     CDS             complement(3410..6175)
                     /locus_tag="PLB25_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HOE41548.1"
                     /translation="MVNQTDLRPDPDALLAQLRDEDQRAKQGKLRIYFGASAGVGKTY
                     AMLSAAQRAAQAGQDVLIGLVETHGRQETQELLLGIKRLPLRELPYRGQSLQEFDLDA
                     ALAAHPQVLLVDELAHSNAPGSRHPKRWQDVEELLEAGIEVWSALNVQHLESLNGTVG
                     AITGVRVNETVPDTVLDRADEIILVDVTVDELLSRLKAGKVYLPQQAERATQNFFRKG
                     NLIALREIALRRTAEHVGDDVRTFRTEQFRVNRSGTNSTPAWNTSGAMLVCVGPHEGA
                     EHAVRSAARLAGQLNVRWHAAYVETPALQRRSATERDRTLAVLKLAEELGAKTAVLAG
                     TQVARELVQQAQKLNCATLVIGRPQHASWQNLWHLFRPTLTRQLATLAPAIDMVEVGT
                     PPAMRRISPQPLQAASVASDATAPWQQALPRYAWAMAACLLTTLLTAPLQKFLDLANI
                     VMLFLLCVVLVAVRLGRGPAMLAAVVAVAAFDFFYVQPHLSFAVSDVQYLVTFAVMLA
                     VGLIIGQLTARLQFAADVSASRERRASALFELTRELSAALQTDQIVELGEAAVQQTFG
                     GQARVLLTNANDQLALGDGVPDGPDASIADWTFNHGQRAGLATATLPSSRWHYVPLRA
                     PMRIRGVLALAPSHPRWLLIPEQVQQLDTLARQIAIAVERVHYVEIAQAAVVQMESER
                     LRNTLLAAMSHDVRTPLTALVGMAESLDQSAPLTAEQRASTQAIRSEAHELSALVSNL
                     LDMARLESGQVNLHRDWQSVEEVVGAAIRAARHALGELEVRTEIAQDLPLVDFDASLM
                     ERVLVNLLENAAKYGVSPNTAQPQPIVVSARVSATSLELQVRDHGPGLPLASRGQEET
                     LFDKFTRGQTESATRGVGLGLAICKAVVQAHHGSITAAQATGGGAEFTITLPRTPAPE
                     LPLTEEN"
     gene            complement(6469..7041)
                     /gene="kdpC"
                     /locus_tag="PLB25_07975"
     CDS             complement(6469..7041)
                     /gene="kdpC"
                     /locus_tag="PLB25_07975"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HOE41549.1"
                     /translation="MKTILRPALVLFFILTLLTGVVYPLIVTGLAQALFPAQAGGSLI
                     LRNGKPVGSALIGQNFTDPKHFWGRPSATGPMPYNAQASGGSNMGPLNPALAAAVKAR
                     IAALRSADPHNTAVIPVDLVTASASGLDPHISLAAAQYQLARVAAVRQLPVATVQALI
                     DQHVQRPWPAWLGEPVVNVLSLNLALETAQ"
     gene            complement(7080..>8306)
                     /gene="kdpB"
                     /locus_tag="PLB25_07980"
     CDS             complement(7080..>8306)
                     /gene="kdpB"
                     /locus_tag="PLB25_07980"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HOE41550.1"
                     /translation="AGMSRMMQANVIATSGRAVEAAGDVDVLLLDKTGTITHGNRQAT
                     AFLNAPGVTPQRLATVAAQASMTDETPEGRSVVALAQRLFTPEPSTGEGQDVPFSAQT
                     RMSGVDIRHPDGSTQQLRKGAVDAVRRHIEAQGGQLPTEVARTADEVARRGSTPLAVC
                     EGCTVLGIVELKDIVKSGIKERFIELRRMGIRTVMITGDNKLTAAAIAAEAGVDDFLA
                     EATPEDKLKLIRQYQASGQLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVD
                     LDSNPTKLLEIVETGKALLMTRGSLTTFSIANDVAKYFAIIPAIFVSTYPQLAALNLM
                     QLGSPSSAILSAVIFNALIIVFLIPLSLKGVKYRAMGASALLRRNLLIYGAGGLIVPF
                     VGIKAIDLLLVAMHLA"
BASE COUNT         1626 a         2508 c         2547 g         1625 t
ORIGIN      
        1 ttggtgacaa aaaagcagtg gtcggcgttg aagatgcgcg ccgcattgcg ctcgcttttg
       61 ccaatggctc cgtcatggtc gagcaactga ccgagttctt ccaccgcgtt gcagacatcg
      121 gcgcgcaaca tgttttcgcc ataaaactgg tggtacatct ggcccaccgg actcttcagg
      181 aaagccacgc cacccgagtg acccgggcaa tgccatgaat acgagccgtc ttcggcatag
      241 tccagtagtg ccttgaaaaa gggcggttgc acaccctcaa ggtagctttt ggcttcgcgg
      301 atgatgtggc gggcgacaaa ctcgggcgtg tcctcgaaca tgtggatgaa accgtgcagt
      361 tcacgcaaga tgtcgttggg caaatgccgc gaggttttgg tctccccgta aatgtagatc
      421 ggcacatcga ggtttttgcg cctgacttcc tcgatgaagt ggcgcaagtt caacacggca
      481 gggtccagat ccggccccgg cgtgaattcc tcgtcgtcaa tcgacaaaat aaacgcgctg
      541 gcacggcttt gctgctgggc aaactggctc aaatcaccat agctggtgac acctagcacc
      601 tcgaaacctt ctgattcaat ggcgtgcgcc agcgcccgaa tacccagtcc ggatgtattc
      661 tcagaacgga aatcttcgtc aataatgaca atggggaagc gaaatttcat ggtgggctcc
      721 ggggtcgcag gctaaatatt caaaattggc gcgaagtgta aggactaatc cggatgcctg
      781 agttcttgcg ccgggctttc gcctgaaaat ggcgtggtcg caacacggtg aaggcgaata
      841 tggctgattc aacactttgg tggctggctg caggcgcact gatcgcggca gagctgatta
      901 ccggcacctt ctacctgctc atgctgtcaa ccgggctggc tgcggccgcg ctggtagcgc
      961 acgccgggct gggcatgccc tggcagtggg ccagcgccgc agcggtaggc tgcggctcgg
     1021 tgctggcatg gcgcagtctg aaacaatcgc ggccctcgaa tgcacctgcc ggggccaatc
     1081 atgatgtcaa catggacata ggcggcacag tgcaggtagc ggcctggcgc gaagatggca
     1141 cctgcagcgt caagtaccgc ggtgcgcaat gggacgctgc cttgcaggcg ggtgaaccag
     1201 cacaggccgg ttcccacgtc attgtcgaag tgattggcag ccgcctgatt ctgaaaaaac
     1261 aacccaactc ttaaccactg gagatcacca tggacattct tgccatcgtt cttttcgtca
     1321 ttgccgtgat ttttatcacc cagtcgatca aggtcgtgcc gcaacagcac gcctgggtgg
     1381 tcgagcgcct gggcaaatac aacggcaccc tgacaccggg cctgagcttt ctgatgcctt
     1441 tcatcgacaa ggttgcctac aagcacctgc tcaaggaagt gccactggac attgccagcc
     1501 aggtctgcat cacccgcgac aacacccagt tgcaggtgga cggcattttg tactttcagg
     1561 tcaccgatgc gatgcgggcc agctatgggt caagcaacta catcgtggcc atttcgcaac
     1621 tggcacagac ttcgctgcgc tcggtgatcg gcaagcttga actcgacaaa acctttgaag
     1681 agcgcgacat catcaacgcc caggtggtgc aagcgattga cgaggcggcg ctgaactggg
     1741 gcgtgaaggt gctgcgctac gaaatcaagg atctgacacc gccaaaggaa atcttgcatg
     1801 cgatgcagca gcagatcacc gccgagcgtg aaaagcgcgc cctgattgcc gcctctgaag
     1861 gccgcaggca agagcaaatc aacattgcca ccggcgagcg cgaggccttt atcgcccgct
     1921 ccgaaggtga aaagcaggcc gtcatcaaca aggcgcaagg cgaagcggcg tccattcttg
     1981 ccgttgccga agcgactggt caagccatag agcgcgttgc tgccgccatt cgccaacccg
     2041 gcggtgaaca ggcagtgcag ctcaaggtgg ctgaaaaggc ggtcgaagcc tatgggcgcg
     2101 tggcctccga tgccaccacc acgctgatca ttcccggcaa catgaccgaa gtgtcggcgc
     2161 tgatcagctc ggccatgaag atgattcaga cgcagaagta aagtgggcta tcggccagac
     2221 tgactggtta actgtggacc ttcacggtca cgtgtttgcc atgcaagtga aatatgggtc
     2281 tagcccttgt cctgtaagcg ctgatagcta tcttgtttat agtgctctga ggtctttgat
     2341 ctctgacaag taaagtctta gcactttccg agaagatttc aggaaagtca tgaagtcatt
     2401 cgtcgggacg gcgcaactcc cacaaactca caatgctccc gctatgttct gtgggagtcg
     2461 cgccctcgcg acaaaggctc ttcgattccg ccttgaccgg cttaggtgca agcgctggta
     2521 tggcaccggt cgtctaccag gctgatgctc aagccgcctg aaagtgatcg ccatcaggct
     2581 cgcagtgcgt gctattgctg atgatgaaat acggctctag cccttatgaa gaaagcgcgt
     2641 ccagctatca ttgttgtaac accaggatac cctgtgccgg gccagctcaa ctgtctggca
     2701 gcaagcggta gccgaccgcc gtctcggtca gcaagtgctg cggctgtgcc ggatcgactt
     2761 cgagtttttg acgcaggtga cccatgtaaa tgcgcaggta gtggctttgg tcggcatgtg
     2821 ccgggcccca cacgtcacgc atcaactggc ggtgggtcat gacccggccg gcattggcca
     2881 tcagcacggt gagcaagcgg tactcaatcg gcgtcaggtg gatggccgtg tgattgcgcc
     2941 gcacgattct ggcctgccgg tccacttcga cttcgccaaa cctgaacaag ggatcaagtt
     3001 cgcccgacct ggcatccatc tggccacctg ccgtgctgcg cggtcggcgc aaattggccc
     3061 gcacccgtgc cagcagctcg ccgatgccaa agggcttggt cagatagtcg tcagcaccag
     3121 catcgagtgc ggcaatcttg tcaccctcgt cagtgcgggc cgacaaaacc atgatcggga
     3181 cgttggacca gccgcgcacc tcccggatca aatcgagccc gtcaccatcg ggcaggccca
     3241 ggtcaagcac cagcaaatcg ggcttgcggg tggctgcatg ctgcaaaccg gttctgacgt
     3301 tctcggcctc gaacaccagc cagccttcgg actccagcgc ggcgcgcaca aagcggcgaa
     3361 tctggggttc gtcttcaatc aggatggcaa ccggggcaga cattggcgtt cagttttctt
     3421 cagtcagggg cagttctggc gcaggcgtgc gtggcagggt gatggtgaat tctgcaccac
     3481 caccggtcgc ttgtgcggcg gtgatgctgc catgatgcgc ctgcaccacc gccttgcaga
     3541 tggccaggcc caagccgaca ccacgggtgg ccgactcggt ctggcctcgg gtgaacttgt
     3601 caaaaagggt ttcttcctgc ccccggctgg caagcggcag gcccggccca tggtcacgca
     3661 cctgcagttc caggctggtg gcgctcaccc gcgcactcac cacaatgggt tgtggctgcg
     3721 ccgtgttggg cgacacgcca tatttggcgg cattttccag caggttgacc agcacccgct
     3781 ccatcaggct ggcatcaaag tccaccagcg gcaaatcctg cgcgatctcg gtgcgcacct
     3841 ccagttcgcc cagcgcatgg cgtgcggcac ggatcgccgc acccaccact tcctcgactg
     3901 actgccagtc gcgatgcaga ttgacctggc cgctttccag tcgtgccatg tcgagcaggt
     3961 tgctcaccaa ggcgctcagc tcatgggcct cgctgcgaat ggcctgggtc gatgcccgct
     4021 gctcggctgt cagcggggcc gattggtcca gagattcggc catgccgacc agcgccgtca
     4081 aaggtgtgcg cacgtcatgc gacatggcgg ccagcaaggt gttgcgcagt cgctcggact
     4141 ccatctgaac cacagcggcc tgggcaatct cgacatagtg aacccgttcc acggcaatcg
     4201 caatctggcg cgccagcgtg tcgagctgtt gcacctgttc tggtatcagc agccagcgcg
     4261 ggtggctcgg tgccagcgcc agcacgccac gaatgcgcat cggtgcccgc agcggcacgt
     4321 aatgccagcg gcttgatggc agggtggcgg tggccagccc ggcgcgttgg ccgtggttga
     4381 aggtccagtc ggcaatgctg gcatcgggcc cgtctggcac gccatcgccc agtgccagtt
     4441 ggtcattggc attggtcagc agcaccctgg cttggccgcc aaaggtttgc tgcacggcgg
     4501 cttcgccgag ttcgacaatc tgatcggttt gcagggcggc agacagttcg cgtgtcaatt
     4561 caaacaatgc gctggcgcgg cgctcacggc tggctgacac atcggcggca aattgcaggc
     4621 gggcggtgag ttgcccgatg atcagcccga ctgccagcat cactgcaaaa gtgaccaggt
     4681 attgaacatc actgacggca aatgagagat gtggctgcac ataaaaaaag tcaaacgccg
     4741 ccaccgcgac caccgctgcc agcatcgccg ggccacggcc cagccgcacc gccaccagca
     4801 ccacacacag cagaaacagc atgacgatat tggccaagtc cagaaacttc tgcaggggcg
     4861 ccgtcaacag cgtcgtcagc aggcaggccg ccatggccca ggcgtagcgc ggcagggctt
     4921 gctgccacgg cgcggttgca tcactggcaa cactggcggc ttgcagtggc tgcggtgaaa
     4981 tgcggcgcat ggccgggggc gtgccgactt ccaccatgtc gatggccggt gccagcgtgg
     5041 ccagttggcg cgtcagggtt gggcgaaaca gatgccaaag gttttgccaa ctggcatgct
     5101 gtgggcgacc gattaccagg gtggcgcagt tcaatttttg cgcctgctgc accagttctc
     5161 gcgccacctg ggtgccagcc agaaccgccg ttttggcacc aagttcttca gccagtttaa
     5221 gcaccgccag cgtgcggtcg cgttcggtcg cgctgcgccg ctgcagcgcc ggggtttcca
     5281 cgtaggccgc atgccagcgc acattgagct gtccggccag ccgggcggca ctgcgcacag
     5341 catgttctgc gccttcatgc ggtccgacgc agaccagcat ggcaccggat gtgttccacg
     5401 ccggtgtgct attggtaccg ctgcgattga cgcgaaattg ttcggtgcga aaggtgcgca
     5461 catcgtcacc cacatgctcg gcggtgcgcc gcagggcaat ttcgcgcagg gcaatcaggt
     5521 tgcctttgcg aaagaaattt tgcgtggcac gctcagcttg ctgcggcaga tagactttgc
     5581 cggctttcag gcgactgagc aactcatcga cggtgacatc gaccaggatg atttcatcgg
     5641 cgcggtcaag caccgtatca ggcacggtct cgttgacacg aacgccggtg atcgcaccca
     5701 ccgtgccgtt gaggctttcc agatgctgca cattgagtgc tgaccagact tcaataccgg
     5761 cctccagcaa ctcttctaca tcctgccagc gcttggggtg gcgcgaaccc ggcgcattgc
     5821 tgtgtgccag ttcgtccacc agcagcacct gcgggtgcgc ggccagcgcc gcgtccaggt
     5881 cgaactcctg cagcgattgc ccccggtaag gtagctcgcg caatggcagg cgtttgatgc
     5941 caagaagcag ttcctgcgtt tcctggcggc cgtgagtctc caccagaccg atcaagacat
     6001 cctgcccggc ttgcgcagca cgttgcgccg cgctgagcat ggcgtaggtt ttgccaacac
     6061 cggcactggc accaaaatag atgcgcagct tgccctgctt ggcgcgctga tcctcgtcgc
     6121 gcagttgggc cagcagcgca tcggggtcgg gtcgcaagtc ggtctggttg accatgttcc
     6181 taggggcctg tcggagctgt ggggcagtgg ctgggagccg accgcagtgg cctattttgc
     6241 accggcttgt cgacccatgg gcgggctatg ggccgaccct tccggcccaa tctggcctcg
     6301 cctggatcga ttttggcgat tgatgccata ggtctgagtg gctcctgatg ttcatcaaag
     6361 ttggatgcct gccggttgcc aaatgtcagc agcagccagg ctaccagcat gggaatcagc
     6421 aacacaccca gtgccatcaa aatgacggtc cagtcctgca ccatagcgtt actgtgccgt
     6481 ttcgagcgcc aggttcaatg acagcacatt gaccactggc tcgcccaacc aggctggcca
     6541 gggacgttgc acatgttggt caatcagcgc ctgaacggtc gcaaccggaa gttggcgcac
     6601 agcggccacg cgggccagtt ggtactgtgc tgccgccagg ctgatgtgcg ggtccagccc
     6661 gctggcagaa gccgtcacca gatcaaccgg aatgactgcc gtgttgtggg ggtcggcgct
     6721 gcgaagcgcg gcaatgcgag ccttgaccgc tgccgccagt gctgggttta gcggccccat
     6781 gttggagccg cccgatgcct gagcgttgta gggcatcggt ccggtggccg acgggcgacc
     6841 ccagaagtgt ttggggtcgg tgaagttctg gccaatcagg gcagaaccca ccggcttgcc
     6901 gttgcgcagg atcaggctac cgccggcttg cgccggaaac agcgcctgcg ccaggccggt
     6961 gacgatcagg ggatagacaa cgccagtcag cagggtgagg atgaaaaaca gcacaagtgc
     7021 cggacgaaga atggttttca tgaggtttcc ttcaattttg gtgatggcgc agtcaggctt
     7081 caagccagat gcatggccac cagcagcaaa tcaatcgcct tgatgccgac aaacggcacg
     7141 atcaggccac ctgcgccata gatcagcagg ttgcggcgca acaaggcact ggctcccatg
     7201 gcgcggtact tgacgccttt gagcgagagc ggaatcagga acacgatgat cagcgcgttg
     7261 aagatcaccg ccgacaggat ggccgacgac gggctgccca gttgcatcag attgagcgcc
     7321 gccagttgcg ggtaagtgct gacaaaaatc gccggaatga tggcaaagta cttggccaca
     7381 tcgttggcaa tcgaaaacgt ggtgagcgag ccgcgcgtca tcagcaacgc cttgccggtc
     7441 tcgacaattt ccagcagctt ggtcggattc gagtccagat cgaccatgtt gccggcttcc
     7501 ttggcggcct gggtgccgct gttcatggcc accgccacat cggcctgagc cagtgccggg
     7561 gcatcgttgg tgccgtcacc ggtcatcgcc accaactggc cagaggcctg gtactggcga
     7621 atcagcttga gcttgtcttc gggcgtggcc tcggccagaa agtcatcgac accggcttcg
     7681 gcagcaatcg ccgcagcggt gagcttgttg tcgccggtga tcatgacggt tctgatgccc
     7741 atgcggcgca gttcgatgaa acgctccttg atgcctgact tgacaatgtc cttgagttcc
     7801 acaatgccaa gcaccgtaca gccttcgcag accgccagcg gcgtgctgcc acggcgtgcc
     7861 acctcgtcag cggtacgcgc cacctcggtc ggcagttggc cgccctgggc ttcgatgtgc
     7921 ctgcgcaccg catccaccgc acccttgcgc aattgttgtg tgctgccgtc cgggtgccgg
     7981 atatcgacac cgctcatgcg cgtttgcgct gaaaacggca cgtcctggcc ttcccctgtg
     8041 ctcggttcag gagtaaacag gcgctgtgcc agcgcaacca cgctgcggcc ttcgggggtt
     8101 tcgtcggtca ttgacgcctg tgctgccacg gttgccaatc gctggggtgt cacgcctggc
     8161 gcattcagga aggcggtggc ctgccggttg ccatgggtga tggtgccggt tttgtcgagc
     8221 agcagcacat cgacatcacc ggcggcctcg accgcgcggc ccgaggtggc aatcacattg
     8281 gcctgcatca tgcggctcat gccggc
//
LOCUS       DAOSLL010000139         8277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8064_length_8277_cov_4.993919, whole genome shotgun sequence.
ACCESSION   DAOSLL010000139 DAOSLL010000000
VERSION     DAOSLL010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8277
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8064_length_8277_cov_4.993919"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            124..1170
                     /locus_tag="PLB25_07985"
     CDS             124..1170
                     /locus_tag="PLB25_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HOE41551.1"
                     /translation="MSTIHYTRGQELPDILQKRIMVLDGAMGTMIQRFKLGEAQYRGE
                     RFKDFHKDIKGNNELLSLTRPDVITDIHERYLAAGADMIETNTFGATAIAQADYAMAE
                     LAFEMNLVSARLARAACDKFSTPDKPRFALGALGPTPKTASISPDVNDPGARNVTFEE
                     LRAAYYEQVEALVQGGVDVLLVETIFDTLNAKAALFAIDEFFEASGERLPIIISGTVT
                     DASGRILSGQTVTAFWHSVRHAQPLAIGLNCALGATLMRPYIQELNRAAPDTFISCYP
                     NAGLPNPMSDTGFDETPEVTSRLIREFATEGLVNIVGGCCGTTPAHIDAIHQAVAPLA
                     ARGLRKSYFYQAAA"
     gene            complement(2073..2816)
                     /locus_tag="PLB25_07990"
     CDS             complement(2073..2816)
                     /locus_tag="PLB25_07990"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE41552.1"
                     /translation="MSLPNDRHHEQWENLGSHDPYWAVLSDPKMKGGKWDKVDFFESG
                     RCEIEQVMKNLTHISVIPVFGIALDFGCGVGRLSRALSQHFNRVVGIDISKSMLEEAR
                     AQHSDFPNIEFLHNVGENLGSISSNTIDLVYSNIVLQHMPSERQLQFICEFCRVLRPG
                     GVAIFQTPSQHDFGVLTGWVHFLVGNRLLNIVRSAIYGKHGIMEIHTLPKATVLATFD
                     ALGMSIADIERYDSSGKGFVSYRYYAVKA"
     gene            2960..3151
                     /locus_tag="PLB25_07995"
                     /pseudo
     CDS             2960..3151
                     /locus_tag="PLB25_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4126..4470)
                     /locus_tag="PLB25_08000"
     CDS             complement(4126..4470)
                     /locus_tag="PLB25_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41553.1"
                     /translation="MSAMQSGVTDNLINALRLSLGVARRTLQRWRHWWREIFVGTTFW
                     TLGSGRFMPPVEHAALPDSLLERLGGMDARSQLVRCLNFWHHFQNLAVSPTMMVAEHP
                     QRMSQPRDALRR"
     gene            5018..6130
                     /gene="queA"
                     /locus_tag="PLB25_08005"
     CDS             5018..6130
                     /gene="queA"
                     /locus_tag="PLB25_08005"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HOE41554.1"
                     /translation="MTALIPGAERSFTLSDFDFELPAELIAQHPAPERSGSRLLDASG
                     PAPVDRIFRELPSLLQPGDLLVFNDTKVMNARLFGEKPTGGKVELLIERVLGGNQVAA
                     HMRVSKKPEPGTTIKLNAAPGSNDHLSATLLGRWPDQQGALFRFVLCNDDGDDPYTLM
                     ARHGHVPLPPYISHADSAHDAQRYQTVFANKPGAVAAPTAALHFDLALLSAIDAIGVQ
                     RAYVTLHVGAGTFQPVKTENLAEHQMHSEWYEMPAATQQAIADCRARAGRIIAVGTTT
                     LRTLESWALEKGTGAAFSSETRIFITPGFEFKLVDVLLTNFHLPKSSLMMLVSAFAGY
                     PCVRKLYDHAIARGYRFFSYGDAMLLVRSVGTSAGK"
     gene            6161..7423
                     /locus_tag="PLB25_08010"
     CDS             6161..7423
                     /locus_tag="PLB25_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOE41555.1"
                     /translation="MNSFQKRTALQIATVSIVLASLASPVAWFVERERSEEGIVALAI
                     EESGRLLHHYDAVDLSGPNALEHAALAAKTISGGMFDIAEIYDPQGEKLAESLTKEGA
                     AVESSLPKHGRPIYNISSYESLRLPGDAWILRVFVPLRESISDRSLPITGYFEGVRLV
                     PQWQRDQIRNSSLTVALMVCLASLLCGAVLYPVVVRLSADNERKTREVLDSHISMMEA
                     LGRAIAKRDSDTGAHNYRVAWIAASIAEKMGFEGNAMQALIAGSFLHDVGKIGVPDAI
                     LLKPGKLDDTEMAIMRTHVSQGKEIVTGMGWLDGANAVVASHHEKWNGSGYPQRLQGE
                     SIPLPARIFAVADVFDALCSKRPYKEPMGFDATMSILEKDTGSHFDPAVMAVFKPMAQ
                     GIFDRLAGCSEAQARQLLQDRVRQHFEM"
     gene            7557..>8277
                     /locus_tag="PLB25_08015"
     CDS             7557..>8277
                     /locus_tag="PLB25_08015"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HOE41556.1"
                     /translation="MLKFDLLATDSANGIYPGSHARRGRLTLSHGVVQTPIFMPVGTY
                     GTVKGVMPCSLEDMGAQIILGNTFHLWMRPGQAVMQSFGGLHDFERWHKPILTDSGGF
                     QVWSLGAMRKITEQGVHFASPVNGDKLFMSPEVSMQIQTTLNSDIVMQFDECTPYLSV
                     EPKTKGQITTEREARVSMEMSLRWAKRSQHEFARLENPNALFGIVQGGMFEHLRQNSL
                     VALVEMNFPGYAIGGVSVGEPK"
BASE COUNT         1755 a         2327 c         2358 g         1837 t
ORIGIN      
        1 tgtgcgccca aatccgccgg tgcaaatagg ggaagttatt tctgaccaca tcctatgcag
       61 gcaaaccggt tcaggccgca agccgacaac ggttgagcca gtgcctttct acaataccgc
      121 cccatgagca caatccacta cacccggggc caggaactgc ccgacatcct gcaaaaacgc
      181 atcatggtgc ttgacggtgc catgggcacc atgatccagc gcttcaagct tggcgaagcc
      241 caataccggg gcgagcggtt caaggacttc cacaaggaca tcaagggcaa taacgagttg
      301 ctgagcctga cgcgcccgga tgtcatcact gatatccatg agcgttacct ggccgctggt
      361 gccgacatga tcgagaccaa cacctttggt gccaccgcca tagcccaggc ggattacgcc
      421 atggccgagc tggcattcga gatgaacctg gtatcggcca ggctggcccg cgccgcctgt
      481 gacaagtttt ccacccccga caagccgcgc tttgccttgg gcgcgctggg gcccacaccc
      541 aagaccgcca gcatcagccc ggacgtgaac gaccccggcg cacgcaacgt cacctttgag
      601 gagctgcggg cggcttacta cgaacaggtc gaggcgctgg tgcagggcgg ggtcgatgtg
      661 ctgttggtgg aaaccatttt tgacacgctc aacgccaaag ctgcgttgtt tgccattgac
      721 gagtttttcg aagccagtgg tgagcgcctg ccgatcatca tcagcggcac ggtgaccgat
      781 gcctccgggc gcatcctgag cggtcagacc gtgactgcct tctggcacag cgtgcgccac
      841 gcccagccgc tggcgattgg cctgaactgc gcgctcgggg cgacactgat gcgcccgtat
      901 attcaggaac tcaaccgcgc cgcgccagat acctttatca gttgctaccc aaatgcgggt
      961 ttgcccaatc cgatgagcga caccggtttt gacgaaacgc cagaagtcac cagccgtctt
     1021 atccgcgaat ttgcgaccga aggtctggtc aacatcgtcg gtggctgctg cggcaccacg
     1081 ccagcccata tcgatgccat tcaccaggca gtggcaccgt tggctgcacg ggggctgcgg
     1141 aagagctatt tttaccaggc ggctgcctga cggtttggcc ttgatgccag ggttggtggt
     1201 cggcgggtat cgtcggcatt ggcatggtca gccgaagccg cgaatgtgcg gtcgtggtgg
     1261 ttttggtcgt ggttgccaac attgggcacc ttggcgaccg acatgccggg cggtgcatca
     1321 gtttcgacaa tcttgaaatt ggcttgtccg aaagcgacca ctggtggtcg gctgctgccg
     1381 ctgtcacctg gtggggcatt tttggggcat ttgtggcgcg ctgcggggtg ccgcagggtt
     1441 aggcatcaaa ttggcttcta gcgcatacgg gataagcgct ggcagctatt gtttttgatt
     1501 tgtttggggc aatgtgattt ttctgcctgg ctggaagtcg gcccaagctg gctacgttgc
     1561 tcgaaatgtc acatcgcagc cagccgctca gatttgttgg ccgtctgaga attttttctt
     1621 tccggcgatt catgctgcgc cagcgggggt tgtgggtcgg gattcctggt ggtgctacga
     1681 gttgatgctg cgcagcagcc agacagggcg gcccctcata ctggcgtacc agaaatcggg
     1741 gccgccccgt ctgactgctg cgccgatgct gttgattgcg cgcaagggga gtttgaatac
     1801 cgcatgctgt acgtcagcgg cgtttagcgg atggtggtgg cagaaacaat gttgggtgcc
     1861 tggcattcac atgtcaaatt ggcctgtagc ctttgtttta taagcgctgc atgctctttt
     1921 acttgtagct taaggcgttt tgcttttttg gaagttgacg gacttcttga gaccggttcc
     1981 ggtcattcga attgacacag ttatgtggaa cacaaaactg agtagagctg aagccggtgc
     2041 gtaccggagt tgctttgcga tttagcccct cattaggcct tcactgcata gtaacgatag
     2101 ctcacaaaac ctttgcccga agagtcatac ctttcgatgt cagcaataga catacccaat
     2161 gcgtcaaacg ttgccaggac agtggctttg gggagtgtat gaatctccat aattccgtgt
     2221 ttcccataga tcgcactcct aacaatattc aaaaggcgat tgcctacaag aaaatgaacc
     2281 cacccagtca atacaccgaa gtcatgctgg gagggtgttt gaaagatcgc aactccacca
     2341 ggacgtaaaa ctcgacaaaa ctcgcagatg aactgaagtt gtctctcgga aggcatatgc
     2401 tgcaaaacga tattgctgta gacaaggtca atcgtgttgc tcgaaattga acccagattc
     2461 tccccaacgt tgtgcaggaa ttcgatgttt gggaagtcac tatgctgggc tctcgcttct
     2521 tcgagcatcg atttcgaaat gtcgatccca acaactctat tgaagtgttg tgacaaagcg
     2581 cgacttagtc gaccaacgcc acacccaaaa tccagcgcga tgccgaagac tggaatcacc
     2641 gaaatgtgag tgaggttctt cataacctgc tctatttcgc aacgtccaga ctcaaaaaaa
     2701 tcaactttgt cccattttcc tcccttcatt ttcggatctg acaacacagc ccaataggga
     2761 tcatgagacc caaggttctc ccattgctca tggtggcgat cattcggcaa gctcatccgg
     2821 aaaccctaga aattcgaaaa cccagtttag cccactgaac gcccgggaat ttcagctccc
     2881 gctgcaaaac tgtcagtgaa atcagttgga tacgttcgag cggttgttgc ccgatatgaa
     2941 gcgagctctt ccaatagcgc acctgccacc cgatgtgaag caactcgcgc cgcgtgatgc
     3001 ggaagtgatg gacgagctgt ttgccacctt tgaccctgcc atgttgaccg gcatttggtg
     3061 catcaacatt atgctgctgc tgttcaagga ctttttgcac cccttcgcca tgcttggcgc
     3121 gctgccgcgg tcatctggtg acgcatttgt ggcgcgcggc ggggtgccgc agggttaggc
     3181 atcaaattgg cttctagcgc atacgggata agcgctggca gctattgttt ttgatttgtt
     3241 tggggcaatg tgatttttct gcctggctgg aagtcggccc aagctggcta cgttgctcga
     3301 aatgtcacat cgcagccagc cgctcagatt tgttggccgt ctgagaattt tttctttccg
     3361 gcgattcatg ctgcgccagc gggggttgtg ggtcgggatt cctggtggtg ctacgagttg
     3421 atgctgcgca gcagccagac agggcggccc ctcatactgg cgtaccagaa atcggggccg
     3481 ccccgtctga ctgctgcgcc gatgctgttg attgcgcgca aggggagttt gaataccgca
     3541 tgctgtacgt cagcggcgtt tagcggatgg tggtggcaga aacaatgttg ggtgcctggc
     3601 attcacatgt caaattggcc tgtagccttt gttttataag cgctgcatgc tcttttactt
     3661 gtagcttaag gcgttttgct tttttggaag ttgacggact tcttcagacc cttatgcaca
     3721 tttcccagtt attgacagta tgacggcatc gattgctttt tccactgtgc agcgcgaacc
     3781 aatccgggga atgaggttga catcatttca gggttggtgc atgggtagca cctcgatacg
     3841 tgcgccagaa ccgtggctcg ctcagatacc gaaagaccat gactttggtt catatgccgc
     3901 tgatatgccg ggtgtgtttg gtgaatcagt acaaatgaac tggccagatg tgatggtcga
     3961 gaaagtcctt tgattatgtt cagaagttct agcgatgacg tgctctttac gtgggacgat
     4021 tccacatgcg agcccacaaa tgttcataac tgggaaatgt gcataacagc ctgatccgga
     4081 agtcgaaaca gaggctaccg tgctacgttt ttgacatcgc cagcatcacc ttctgagagc
     4141 atctctgggt tgcgacattc tctgcgggtg ctcagcgacc atcatcgtgg gtgaaacagc
     4201 caggttttga aagtggtgcc aaaaattgag gcacctcacc aactgcgatc gggcatccat
     4261 cccgccaaga cgctccagta ggctgtctgg caaggctgca tgctcgactg gcggcataaa
     4321 acgaccgctg cctaaagtcc aaaacgtggt tcccacgaat atctcgcgcc accaatgccg
     4381 ccatcgctgc aatgtccggc gtgccacccc caggctcaag cgcaatgcgt tgatcaggtt
     4441 gtccgtcacg ccggactgca tggcgctcat caaaaccatt gcaaacccag aatcgacacg
     4501 tcgccccaga aagcgcacag attctgcggt tgttcgcggt ctgcatttgt cacaacaaaa
     4561 actgtgccgc gttgtcgctg cagtcgcctc caattcccct gaccctcgtg gtttacgggg
     4621 atggcaagcg ctgtgaagaa caccaccaca gaagctgtag ccctgaatcc gagcccgact
     4681 tgccaggtct gagtcaatgg ctgcgagaaa ttggcaaaag caaggctgct gcgagatact
     4741 gacggtcatg gatcagtctt gtgaagtgga gctcagtcca gcagtgtcac cgacaaccaa
     4801 atcgtgttta cccaaatttg atcaagatac ccgatggagt cagtcgttcg tccctcacgt
     4861 aacttgatca aaccagcggg aaattccatc atgaaaactg aatgtgctca ggcaggccag
     4921 agcagtccag cctcttcagg ccgaccagct tcggtcgccc tgcgacaatg ccgtcccgaa
     4981 ccaacgtaca ggcgcttgcg cctgcctcgc aagcaacatg acagcattga tccccggcgc
     5041 agagcgcagt ttcaccctca gtgactttga ttttgagctt cctgcggagc tgattgcgca
     5101 gcacccggcg cccgaacgca gtggctcgcg cctgcttgat gccagcggcc cagcgccggt
     5161 ggaccggatt tttcgggagt tgccgagctt gctgcaacca ggcgacctgt tggtgttcaa
     5221 cgataccaag gtgatgaatg cccgcctgtt tggcgaaaag cccaccggcg gcaaagtcga
     5281 gttgctgatt gagcgcgtgc ttggcggcaa ccaggtggcg gcgcacatgc gggtcagcaa
     5341 gaagcccgaa cctggcacaa ccatcaaact caacgccgcg cctggcagca acgaccactt
     5401 gagcgccacg ctattgggac gctggccgga ccagcagggc gcgctgtttc gcttcgtgct
     5461 gtgcaacgat gatggcgacg acccctacac cttgatggca cgtcacggcc acgtaccgct
     5521 gccaccctac atcagccacg ccgactcggc gcacgacgcg cagcgctacc agacggtgtt
     5581 tgccaacaag ccgggggcgg tggcggcacc gactgccgcg ctgcactttg acctggcctt
     5641 gctgtctgcc attgatgcca tcggcgtgca gcgtgcgtat gtcaccttgc acgtgggcgc
     5701 gggtaccttc cagccggtca agaccgagaa cctggccgag caccagatgc acagtgagtg
     5761 gtatgaaatg ccagcagcca cccagcaggc gattgccgac tgtcgcgcgc gcgcagggcg
     5821 catcatcgcg gttggcacca ccaccttgcg cacgctcgaa tcctgggcac tggaaaaagg
     5881 aaccggggca gcattttcct cagaaacccg catcttcatc acgccaggct ttgagttcaa
     5941 actggtggat gtgctgctga ccaactttca cctgcccaaa tccagcctga tgatgctggt
     6001 gagcgcattt gccggatacc cttgcgtcag gaagttgtac gatcacgcca tcgcccgggg
     6061 ctaccggttt ttcagctatg gcgatgccat gctgctcgtt agaagcgtag gcacgtcggc
     6121 agggaaataa cgcagcaccc ggggcttggt gaatttgtaa atgaacagct ttcaaaaacg
     6181 aactgcgctc cagattgcca ctgtcagcat cgttctggcc tctttggcca gcccggtggc
     6241 atggtttgtt gaacgtgagc gctctgaaga gggcattgtc gcgctggcca tcgaagaatc
     6301 ggggcgtctc ttgcaccact acgatgcggt ggacctgagc ggcccgaatg cccttgaaca
     6361 cgccgcattg gcggcaaaaa ccatttccgg cggcatgttt gatattgccg aaatttacga
     6421 cccacagggt gaaaaactgg ccgagtcgct gaccaaggaa ggtgcggcag ttgagtcttc
     6481 tttgcccaag cacggccgac ccatttacaa catttcgtct tatgagagtc tgcggctccc
     6541 aggagatgcc tggattttgc gggtttttgt cccgctgcgc gaatccatca gtgaccgcag
     6601 cctgcccatc accggttact ttgaaggtgt gcgcctggtg ccccaatggc agcgcgacca
     6661 gatacgcaac agttcgctga cggtggcact gatggtctgc ctggcatcgc tgctgtgcgg
     6721 tgccgtgctg tacccggtgg tggtgcgcct gtctgccgac aacgaacgca aaacccgtga
     6781 ggtgctcgat tctcatattt cgatgatgga ggcattgggc cgcgccattg ccaaacgcga
     6841 ttccgacacc ggtgcccaca actaccgggt agcctggata gcggccagca tcgctgaaaa
     6901 aatgggtttt gaaggcaatg ccatgcaggc cttgattgcc ggcagttttt tgcatgatgt
     6961 cggcaaaatc ggcgttccag atgcgatttt gctcaaaccc ggcaagctcg acgacaccga
     7021 gatggcaatc atgcgcaccc acgtcagcca gggcaaggaa attgtcaccg gcatgggctg
     7081 gcttgacggt gccaacgctg tggtcgcctc gcaccacgaa aaatggaatg gcagcggcta
     7141 ccctcagcgg cttcaaggtg aatcgattcc gctgccagcc cgcatttttg ccgtggccga
     7201 cgtgtttgat gcgctgtgct cgaagcgccc atacaaggag ccgatgggtt ttgatgccac
     7261 catgtcgatt ctggagaaag acaccggcag tcactttgac ccggcggtga tggcggtttt
     7321 caagcccatg gcccaaggca ttttcgaccg cctggcaggc tgttcagagg cacaggcgcg
     7381 ccagttgctg caagaccgcg ttcgccagca cttcgaaatg tagtcggccc gaccgcaaca
     7441 cagcgggttt tggtatctcg cacagggtga tgctgggccg cagcaaagcg ccatcgtgat
     7501 ctgaccggtc tgaacaatgg ctggttcgta caatgccgca gccttctgga gcacccatgc
     7561 tgaaattcga cctgcttgcc accgactccg ccaatggcat ttatccaggt tcgcacgctc
     7621 gtcgtggcag actgaccctc agccacggtg tggtgcaaac cccgatcttc atgccggttg
     7681 gcacctatgg caccgtcaag ggcgtgatgc cctgcagcct ggaggacatg ggcgcgcaga
     7741 tcattctggg caataccttc catttgtgga tgcgcccagg gcaggcggtg atgcagagct
     7801 ttggtggcct gcacgatttt gagcgctggc acaaaccgat cctgaccgac tcgggcggct
     7861 ttcaggtctg gagcctcggg gccatgcgca agatcaccga gcagggtgtg cattttgcca
     7921 gcccggtgaa tggcgacaag ctgttcatgt cgcccgaagt gtcgatgcag atccagacca
     7981 cactcaacag cgacatcgtg atgcagttcg acgaatgcac gccctacctg tcagtcgaac
     8041 ccaaaaccaa gggccagatc accaccgagc gcgaagcccg ggtcagcatg gaaatgagcc
     8101 tgcgctgggc caaacgcagc cagcacgaat ttgcccggct ggaaaatccc aacgcgctgt
     8161 ttggcattgt gcaaggtggc atgtttgagc acctgcgcca gaattcgctg gtcgcgctgg
     8221 tcgagatgaa cttccccggc tacgcaattg gtggtgtgag tgtgggtgaa ccgaagg
//
LOCUS       DAOSLL010000140         8248 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8096_length_8248_cov_4.749054, whole genome shotgun sequence.
ACCESSION   DAOSLL010000140 DAOSLL010000000
VERSION     DAOSLL010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8248)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8096_length_8248_cov_4.749054"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..956)
                     /gene="accD"
                     /locus_tag="PLB25_08020"
     CDS             complement(84..956)
                     /gene="accD"
                     /locus_tag="PLB25_08020"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HOE41557.1"
                     /translation="MSWLEKLLPPKIQRSNPADRRSVPEGIWIKCPACDSVLYKSDLE
                     QNQNVCPTCSHHHRIGARARLNVFLDNEGRYEIGQEVLPVDALKFKDSRKYPERLKEA
                     LESTGETDAMVVMGGSVMGIGLVAACFEFEFMGGSMGSVVGERFVRGVHTAIEQKVPF
                     VCFTATGGARMQEGLLSLMQMAKTNASLTHLAKKHLPYISVLTDPTMGGVSAGFAFLG
                     DVVIAEPKALIGFAGPRVIESTVRVTLPEGFQRAEFLQTKGGVDLICDRRELRQTIAS
                     MLAMLTRQSADAVG"
     gene            complement(1039..1890)
                     /gene="trpA"
                     /locus_tag="PLB25_08025"
     CDS             complement(1039..1890)
                     /gene="trpA"
                     /locus_tag="PLB25_08025"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HOE41558.1"
                     /translation="MSRISTTMGALKAQGRKALIPFVTAGFPYADITPELMHAMVAGG
                     ADVIELGVPFSDPSADGPVIQNAGDKALAFGIGLAEVLAMVLVFRAGDQTTPVVLMGY
                     ANPVERYNQKHGAPAAIAGYAQSTAAVSPFVRDAAAAGVDGMLIVDYPPEECEDFARE
                     LKAHQMDLIFLLAPTSTDARMAQVGQMASGYVYYVSLKGVTGSGALDTASVEAMLPRI
                     RSHVQVPVGVGFGIRDADTARAIGRVADAVVIGSKIIQLIDQQPKSLVGPTAQSFLRE
                     IRCALDS"
     gene            complement(1887..3212)
                     /gene="trpB"
                     /locus_tag="PLB25_08030"
     CDS             complement(1887..3212)
                     /gene="trpB"
                     /locus_tag="PLB25_08030"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HOE41559.1"
                     /translation="MSDYQQPDQRGHFGIYGGSFISETLTHAVSELKRAYARYQFDPA
                     FVAEFESELAHFVGRPSPVYHAARTSRELGGAQIFLKREDLNHTGAHKINNTIGQAML
                     ARRMGKPRVIAETGAGQHGVATATICARYGLECVVYMGSEDIKRQSPNVYRMKLLGAT
                     VVPVTSGSRTLKDALNEAMRDWVANVDNTFYIIGTVAGPHPYPMMVRDFQSVIGKECL
                     LQMPELLTVAGCSSAQPDVVMACVGGGSNAMGIFYPYIGFEKTRLIGVEAAGAGIDSG
                     KHSASIQRGSPGVLHGNRTYVLQDDNGQVTETHSISAGLDYPGVGPEHAFLHDIGRAE
                     YVGITDTEALAAFHYLCRTEGIIPALESSHAVAYAMKLAKTMRTDQSILVNLSGRGDK
                     DIGTVADLSGADFFCRPSCQGQSVKGDVQNVPVALVRQAPVATKSGAIQ"
     gene            complement(3265..4002)
                     /locus_tag="PLB25_08035"
     CDS             complement(3265..4002)
                     /locus_tag="PLB25_08035"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HOE41560.1"
                     /translation="MNTTPPATSVPRPPITPPAGRTRIKMCGLTREQDVDAAVAAGAD
                     AIGFVLYAKSPRFVSPERAAELARRLPPFVLPVLLFVNQDTIKTRAACAMIAGATAQF
                     HGDESPEDCWAGCDDGRRFYLRAARIPLGDAGRSFDLLKFAQDYSQAQGLLLDAHIDG
                     FGGGGQTFNWSLLPPNVNAHLVLSGGLTAANVIDGITQVRPRCKSLAVDVSSGIEVPG
                     RKGIKDPERMQEFVAAVRQADLLLYNK"
     gene            complement(4002..4793)
                     /gene="truA"
                     /locus_tag="PLB25_08040"
     CDS             complement(4002..4793)
                     /gene="truA"
                     /locus_tag="PLB25_08040"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HOE41561.1"
                     /translation="MRLALGVSFNGLAYHGWQSQPCGQTVQDKLEKALGEFSAQKVST
                     LCAGRTDAGVHALMQVIHFDTPLDRPESAWVRASNALLPHDIAVQWAKAMPAQFHCRA
                     SAQSRRYAYILLDSPVRPSIDTGRVGWVYKPLDLERMQQATQYLLGEHDFSSFRASAC
                     QALSPVKHLTRLDITRRGAYWRFEFEANAFLHHMIRNLMGCLVMVGDGRKQPDWVAQV
                     LAARDRDAAAPTFSPYGLYFVGPSYAAHWGLPEHPSLLDGLPGAF"
     gene            complement(4838..7399)
                     /locus_tag="PLB25_08045"
     CDS             complement(4838..7399)
                     /locus_tag="PLB25_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HOE41562.1"
                     /translation="MIAKSHQWRLNAIALAVVLGLWGGNAAALSLGRISVQSALGEPL
                     KAEIDVLDINAEETASFSSKAATPEAFKAAGLDYHAAMANLQASLQRRANGRSFIRLS
                     SDRAINDPFVDLVLEVSWSSGRVVRDYTLLFDPPNLRDKNMAVPTLRQLPGAATAQAG
                     APAPSASREQPSASPNAAETKKPKAVVLSPSKPSARPDGQTVTVKAGDTAGRIAAANK
                     PASVSLDQMLVALLRANPDAFIKDNVNRIKTGSVLALPTEEQAASVSDAEASQVIIAQ
                     SQDFNDFRRKFASSAPLTSVEPVTRDVSGKVQATVQDKKPVPATSDKLTLSKGAVAGK
                     TSEEQLAQAKSARDAASRSAELTKNISDLSKLAAVSGPALAVSASPAVSQAVGPAIKA
                     VPAVSAPSPAPTASMPTPAASMSAPMPAASAAASAPKPAVPAAKKPASAAPVLPAPVA
                     EPGFIDALLENPLLPVGAAGLLALLAGLGIYKFRKRKEAAPIDSSFIEASPDSFLGVS
                     GGQVVDTSDSLTTTGSSMVYSPSQLDDVDEVDPVAEADVYLAYGRDLQAEEILKNALR
                     DNPKRLAIHHKLLEIYAKRHDATAFEAIATQAFNLTDGSGSDWEQICDKGLTIDPDNA
                     LYLPGGQPFEKPSPLTGPAPLDAESGPPTIPFGIYGDADPQPAASAVDIDLDFEFSID
                     EPSAAQSPEATEPLPSTSTDLLEMPPSLDFPEFTKTVPHSIPEPEPEPDPTDDLLAEG
                     LDFSLADSPPAPEFELAAPQVPTSTPANPAPPADADMMSFDLGSLSLDLTDEPVTVAG
                     SLAPEGQDPLDTKLALADEFRAIGDDDGARALIEEVIAEATGEMKAKAQRALSRL"
     gene            complement(7644..>8248)
                     /locus_tag="PLB25_08050"
     CDS             complement(7644..>8248)
                     /locus_tag="PLB25_08050"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HOE41563.1"
                     /translation="AATIKRVVVSTYQSVSGAGKEGMDELFEQSRNIFVGDPAEPKKF
                     TKQIAFNVIPHIDVFLDDGYTKEEWKMVHETNKIMGMGEGFGSPAVPVDGFCVRVGAM
                     RCHSQALTFKLKKDLPVADIEAMIAADNAWVKVIPNTREATIVDLTPVAVTGTMTIPV
                     GRIRKLAMGPDYLGAFTIGDQLLWGAAEPLRRMLRILLQA"
BASE COUNT         1628 a         2464 c         2486 g         1670 t
ORIGIN      
        1 ttgactttga atgtttgaat tcttcgcatg attagcaaag acttcacttg ttggagccca
       61 agctcaacac gccgctgcgc ggttcaaccc accgcatcag cactttggcg cgtcagcatg
      121 gccagcatgc tcgcaatcgt ctggcgcagt tcgcggcggt cgcaaatcag gtccacgccg
      181 cctttggtct gcaagaattc ggcgcgctga aagccttcag gcagtgtcac acgcaccgtc
      241 gattcaatca cccgtggtcc ggcaaagcca atcagcgcct tgggttcggc aatcaccacg
      301 tcacccagaa aagcaaaacc ggcactgacg ccgcccatgg tcgggtcggt cagcacgctg
      361 atgtagggca agtgcttttt cgccagatgg gtcagcgatg cgttggtttt ggccatttgc
      421 atcaggctca gcaagccttc ctgcatgcgg gcaccgccgg tggcggtaaa gcagacaaag
      481 ggcacttttt gctcaatggc ggtgtgcacg ccacgcacaa agcgctcgcc caccaccgat
      541 cccatgctgc cacccatgaa ttcaaattca aagcaggcag cgaccaggcc aatgcccatc
      601 acgctgccgc ccatgaccac catggcatcg gtctcgccag tgctttccag ggcttctttc
      661 aggcgctcgg ggtacttgcg actgtccttg aacttgagcg catccaccgg cagcacctcc
      721 tggccgattt cgtagcggcc ttcattgtcc aaaaagacgt tcagccgggc gcgcgcgcca
      781 atgcggtgat ggtggctgca agtcgggcag acgttctggt tttgctccag atcggacttg
      841 tacagcacgc tgtcacaggc cgggcatttg atccagatgc cttcgggcac gctgcgccgg
      901 tcagccgggt tggagcgctg gattttgggg ggaagcagtt tttcgagcca actcatgctt
      961 gttcctgttc agttgcggac agaattgctt gcctatgcac agcttcagtc tgtccgacag
     1021 atgattgatg tggtcggatt atgaatcgag cgcacagcga atctcgcgca ggaagctttg
     1081 cgccgtcggg ccgaccaggc ttttgggctg ctggtcaatc aactggatga ttttgctgcc
     1141 gatcaccacc gcatcagcaa cccggccaat agcacgcgcg gtgtcagcgt cacgaatgcc
     1201 aaaaccgaca cccaccggga cttgcacatg cgagcggatg cgcggcagca ttgcttcgac
     1261 cgaggcggta tcgagcgcgc cggagccggt cacgcctttg agcgacacgt agtagacata
     1321 gccgctggcc atctggccaa cctgggccat gcgcgcatcg gtgctggttg gggccagcaa
     1381 gaagatcagg tccatttgat gcgccttgag ctcgcgggca aagtcctcac attcttcggg
     1441 cgggtaatcg acgatcagca tgccatcgac tccggccgct gccgcatcac gcacaaaagg
     1501 gctcacagcc gccgtcgatt gggcgtagcc cgctatcgca gcaggcgcac catgcttctg
     1561 gttgtagcgc tccaccgggt tggcgtagcc catcaaaacc actggcgtgg tctgatcccc
     1621 ggcgcgaaag accagcacca tcgccagcac ctcggccagg ccaatgccaa aagccagggc
     1681 cttgtcaccg gcgttctgga tcaccgggcc gtcggcgctg gggtcggaaa acggcacgcc
     1741 cagctcgatc acgtcagccc cgccggccac catggcgtgc atcagctcgg gggtgatgtc
     1801 ggcatacggg aagccggccg tgacaaacgg gatcagcgcc ttgcggccct gcgccttcaa
     1861 ggcgcccatg gtagtgctga tgcggctcat tgaattgctc ctgactttgt agctactggc
     1921 gcttgtctga caagcgctac aggcacattt tgtacatcac ctttgaccga ctggccctgg
     1981 cagcttgggc gacagaaaaa gtcggcacct gacaggtcgg ccaccgtgcc aatgtccttg
     2041 tcgccgcgcc cggacaggtt gaccagaatg gattggtcag ttcgcatggt tttggcaagt
     2101 ttcatggcgt aggcgaccgc gtggctggat tcgagcgccg ggatgatgcc ctcagtacgg
     2161 cacaggtagt gaaaggccgc cagtgcctcg gtatcggtga tgccaacgta ttcggcgcgg
     2221 ccaatgtcgt gcaaaaacgc atgttcgggg ccgacaccgg ggtagtccag cccggcgctg
     2281 atgctgtggg tttcagtgac ctggccgttg tcatcctgca gcacataagt gcggttgcca
     2341 tgcagcacgc cggggctgcc gcgctgaatg ctggccgagt gtttgccact gtcgatgccg
     2401 gccccggcgg cttcaacacc gatcagccgg gtcttctcaa acccgatata cgggtagaag
     2461 atgcccatgg cgttgctgcc accaccaaca caggccatca ccacatcggg ctgcgcactg
     2521 ctgcagccag caacggttag cagttcgggc atttgcagca ggcattcctt gccaatcacg
     2581 ctttggaaat cgcgcaccat catcgggtag ggatgcgggc cggccaccgt gccgatgatg
     2641 tagaaggtgt tgtcaacatt ggccacccag tcgcgcatgg cctcgttgag ggcatccttg
     2701 agcgtgcggc tgccgctggt gaccggcacc acggtggcac cgagcagttt catccgatag
     2761 acattggggc tctggcgctt gatgtcttca ctgcccatgt aaaccacgca ttcgagtcca
     2821 tagcgggcgc agatggtggc agtcgccacg ccatgctggc cagccccggt ttcggcaatc
     2881 acgcgcggct tgcccatgcg cctggccagc atcgcctggc cgatggtgtt gttgatcttg
     2941 tgcgccccgg tgtgattgag gtcttcgcgt ttgaggaaaa tttgcgcgcc gccgagctcg
     3001 cggctggtac gcgcggcgtg gtacaccggt gacgggcggc cgacaaaatg cgccagttcg
     3061 gactcaaact cggcaacaaa ggctggatca aactggtatc tggcgtaagc gcgtttaagc
     3121 tcgcttaccg catgcgtcag cgtctcggag atgaaactgc caccataaat gccaaagtgg
     3181 ccacgctggt ccggttgttg atagtcagac ataagtgtat tttttttgat agcttgcagc
     3241 ccttgttgca caagagctag aagcctattt gttatataaa agcagatccg cctggcggac
     3301 cgccgcaaca aattcctgca tgcgttcggg gtctttgatg cctttgcgcc ctggcacctc
     3361 gatgcccgaa ctgacatcca cggccagcga cttacagcgt ggccgcacct gcgtgatgcc
     3421 gtcgatcacg ttggcagcgg tcagcccacc actcaagacg aggtgagcgt tgacgtttgg
     3481 aggaagcagt gaccaattga atgtttgccc gccgccgccg aacccgtcaa tgtgcgcgtc
     3541 cagcagcagg ccttgagcct gtgaataatc ttgcgcgaat tttaaaagat caaagctgcg
     3601 ccccgcgtcg cccagcggaa tacgcgcggc gcgcaggtaa aagcgcctgc catcgtcaca
     3661 gccggcccag caatcttctg gcgattcatc cccatgaaat tgggctgtag cccccgcaat
     3721 cattgcgcag gcagctcttg ttttgatagt atcttgattg acgaacaaga gcaccggcaa
     3781 gacaaacggc ggcaaccggc gggccagttc tgcggcacgc tcgggtgaca caaagcgcgg
     3841 gcttttggca tacagcacaa aaccaatggc atctgctcca gcggccaccg ccgcatccac
     3901 gtcctgctcg cgcgtcagac cacacatctt gatgcgggtg cgaccggcag gcggcgtgat
     3961 gggtggcctg ggcacagagg tggcaggtgg ggtggtgttc atcaaaatgc tccaggcagt
     4021 ccgtcgagca ggctggggtg ctcgggcaag ccccagtgcg ctgcataact cgggccaaca
     4081 aagtacagcc cgtaaggcga aaacgtgggc gctgcggcat cgcggtcgcg cgctgccagc
     4141 acctgcgcca cccagtcggg ctgcttgcgg ccatcgccca ccatcaccag acagcccatc
     4201 aagttgcgga tcatgtggtg caaaaaggca ttggcctcaa attcaaatcg ccagtaggca
     4261 ccgcggcgag tgatgtccag acgcgtcaga tgcttgaccg gcgacagggc ctggcaggcg
     4321 ctggcgcgaa acgaactgaa gtcatgttca cccagcagat actgcgtagc ctgctgcatg
     4381 cgctcaagat cgagcggctt gtaaacccag cccacacgtc cggtgtcgat actgggccgg
     4441 accggcgaat ccagcaagat ataggcatac cggcgtgatt gcgcactggc ccggcaatgg
     4501 aactgggctg gcatcgcttt cgcccattgc accgcaatgt cgtgcggcaa caaggcgttg
     4561 ctggcccgca cccaggccga ttcaggtcgg tccagcgggg tgtcaaagtg aattacctgc
     4621 atcagggcgt gaacaccggc atcggtacga ccggcacaca gggtagagac tttctgggca
     4681 ctgaactcgc cgagcgcctt ctccagcttg tcttgcaccg tctggccgca tggctggctt
     4741 tgccagccgt gataagccag cccgttgaaa ctgacaccca gcgccagcct cacggggtca
     4801 ggttaccctg gctgtacctg gccatccaac gctgcggtca aagtctgctc aaagcccgct
     4861 gggccttggc cttcatctcg ccagtggctt cggcgatgac ttcctcgatc agggcgcgag
     4921 caccatcgtc gtcgccaatt gcgcggaact cgtcggccag tgccagtttg gtatcaagcg
     4981 ggtcctggcc ctcaggggcc agtgatccgg ccacggtgac aggttcgtca gtcaaatcga
     5041 gtgacagtga acccaggtca aacgacatca tgtcggcatc tgccggcggg gccggattcg
     5101 ctggtgtgct cgtcggcacc tgcggtgcag ccaactcaaa ttccggcgct ggtggactgt
     5161 cggccaggga gaagtccagg ccttcggcaa gcaagtcatc ggttggatcc ggctcaggct
     5221 caggctcagg gatcgaatgc ggaaccgtct tggtgaactc ggggaaatcc agacttggcg
     5281 gcatttccaa caagtcggtg gaagtggacg gcaagggctc tgtagcctct ggtgactgcg
     5341 cggcactcgg ctcgtcgatc gagaattcaa aatcgagatc aatgtcaacc gcactggcag
     5401 caggctgtgg gtcggcatca ccatagattc caaacggaat ggtgggtggc cccgattcgg
     5461 catcgagtgg tgccggacca gtcaatggac ttggcttttc aaaaggctgt ccgccaggta
     5521 gataaagcgc gttgtcaggg tcaatggtca accctttgtc acatatctgc tcccaatccg
     5581 aaccgctgcc atcggtcagg ttgaaggctt gagtggcaat cgcctcgaac gccgtcgcat
     5641 catgccgctt ggcgtagatt tcgagcagct tgtggtgaat ggccagccgt ttcgggttgt
     5701 cgcgcaacgc attcttgagg atttcctcag cctgaaggtc gcgtccatac gccaggtaaa
     5761 catcggcttc ggcaaccggg tcaacctcat caacatcgtc aagctggctg ggcgagtaaa
     5821 ccatcgacga gccggttgtc gtcaggctgt cgctggtatc aaccacctga ccaccactga
     5881 cacccaggaa ggaatctgga ctcgcttcaa tgaaggaact gtcaatcggg gctgcttctt
     5941 tgcgttttct aaacttgtag attcccagtc ctgccagcaa cgcaagcagg ccagcagcgc
     6001 caactggcag caacgggttt tccagcaatg catcgataaa gccaggctca gccacaggcg
     6061 cagggagcac cggtgccgcc gaggccggtt ttttggcagc cgggacggca ggcttgggcg
     6121 ctgaagcagc cgcgctcgcc gctggcatcg gtgccgacat ggacgctgca ggtgtcggca
     6181 tggacgctgt aggtgccgga ctgggggcgg acacggctgg cacggccttg atggcagggc
     6241 cgacagcctg gctgacagca gggctggcac tgacggcaag cgccgggccg gagacggcgg
     6301 caagcttgct gagatcgctg atgttcttgg tcaattcggc cgaacggctg gcggcatcgc
     6361 gggcactctt ggcctgtgcc aattgctcct cactggtctt gcccgctacc gcgcctttgg
     6421 acagggtcag cttgtcgctt gtcgctggca ccggcttctt gtcctgcacg gtggcttgca
     6481 ccttgccgct gacatcgcgg gtgacgggct cgaccgaggt cagcggtgcg ctgctggcga
     6541 acttgcgacg gaaatcattg aagtcctggc tttgcgcaat gatgacctgg ctggcctcgg
     6601 cgtcagaaac gctggccgct tgctcttcgg tcggcaatgc cagcaccgag ccggtcttga
     6661 tgcggttaac gttgtccttg ataaaggcat cggggttggc gcgcaacaag gccaccagca
     6721 tctgatcaag cgacacgctg gctggcttat tggccgccgc aatgcgccct gcggtgtcgc
     6781 cggctttgac agttacggtc tggccgtcag gccgggccga tggcttggac ggcgaaagca
     6841 ctacagcctt gggctttttg gtctcggcgg cattcgggct ggcagacggc tgctcacgcg
     6901 atgcgcttgg tgccggtgcg ccagcctgag ctgtggctgc cccaggcagt tgccgcagcg
     6961 taggcacggc catgttcttg tcacgcaggt tcggcggatc aaacaacaag gtgtagtcac
     7021 gcacgacccg cccggaactc caactgacct ccagcaccag gtcaacaaac ggatcgttga
     7081 tggcacggtc actgctgaga cggatgaagg aacgaccatt ggcacgacgc tgcaatgatg
     7141 cctgcaaatt ggccatggcg gcgtggtagt ccagacctgc cgccttgaag gcttcagggg
     7201 tggctgcttt cgagctgaat gaggcggttt cctcggcatt gatgtcaagg acatcaattt
     7261 ccgccttgag gggttcaccc agtgccgact gcacactgat tcgcccaagc gacaaagctg
     7321 ctgcattgcc tccccataga ccaagtacca cggccagcgc gatggcattc aaacgccatt
     7381 gatgagactt tgcaatcact ttgtaaactc cggttttaca gccaatagca ggcggtgtga
     7441 tgtgggcgtg aaccgccact tctttcaatt ataaaaagca ccatagcatc aacgacatgg
     7501 tctgacaagt tgggactgcc tatgaagttg ttgctatggc ttttgcagat ggtaaagcgc
     7561 gattctatgt ggttgagcaa caacgaacca aagcacctgc gcattttgtc gcaggttcgt
     7621 tcgctaacag gcatagccgc tgctcaagcc tgcaacaaaa ttcgcagcat gcgccgcagc
     7681 ggctcggcgg caccccatag caattggtcg ccgatggtga aggctcccag gtagtctggg
     7741 cccatggcca gcttgcgaat gcgcccgacc gggatcgtca tggtgccggt taccgccact
     7801 ggtgtcagat ccacgatggt ggcctcacgg gtattgggga tcaccttgac ccaggcgttg
     7861 tcggccgcaa tcatggcctc gatgtcggcc accggcaggt cttttttcag cttgaaggtc
     7921 agcgcctggc tgtggcagcg catagcaccc acgcgcacgc agaacccgtc caccggaacg
     7981 gcagggctgc caaaaccttc acccatgccc atgatcttgt tggtttcgtg gaccatcttc
     8041 cattcttcct tggtgtaacc atcgtccagg aagacatcga tgtgcgggat cacgttgaag
     8101 gcgatctgct tggtgaactt cttcggctcg gccggatcac cgacaaagat gttacggctc
     8161 tgctcgaaca gttcgtccat gccttccttg ccggcacccg acaccgactg ataggtggag
     8221 acgacaacgc gcttgatcgt tgcggcat
//
LOCUS       DAOSLL010000141         8245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8101_length_8245_cov_4.384860, whole genome shotgun sequence.
ACCESSION   DAOSLL010000141 DAOSLL010000000
VERSION     DAOSLL010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8245
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8101_length_8245_cov_4.384860"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2379)
                     /locus_tag="PLB25_08055"
     CDS             complement(<1..2379)
                     /locus_tag="PLB25_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41564.1"
                     /translation="MPILTGDIKLVASQVMDDVPEGGGAPTATVITDGTSNAIFPDIS
                     ELDRAGGRVNLRKLHVSVQTMDTDTYMGSNIIVSEPPADPNVSVTLFSTRDTFDRRDA
                     ASARVESYLTKGPMWGGMLLENHIAGQRAVQILQGVDAELPRIGQTMVLVQNEGATNE
                     RNQYIRTTEVSAVKRKFEDSQGKMVDMNVVTCSISDALRTDFQGSEGNAKAAPAAGAT
                     KVRDTTVADAGSYVGVVPLAAAANLGTFSIRATSVYTQLVPSAQTETPLVDLKPNGEQ
                     VVLSAAGGPVTLTTSVALNTSHTISVGQAIMPNSLKLTSGSLTLVDDGGLLSAAGSAV
                     GAVDYANGLISITDPSVSYAGAKTIIYTPAATPVRSLHTASWAVTAESRSSTLVAIFD
                     PAPKPGSFALSYRAQGRWYTLRDAGNGQLRSAFGSVGAGTLNFNTGSMMVTLAALPDA
                     GTQVLATYGLATADTAVYGVAIAAQSVFTLANPGVAPGTVTLTWVTGGVDKTATDNGQ
                     GLLTGDATGKVDYLDGVVTFKPLILPSSGAQVSIEYSWGPPIEENFQAPERFAPDGHI
                     QIVLANPNVLARTVKVEWNTVYDEKDLTIEGQISTRWLSLTYKPRIDPIVIVHDNGAG
                     GFQTRPECAGVINYAAGTLSFKPDTVIALPKPNWTKVVIGTQVIDNAWYTGTLATEKT
                     VFGGFIYQTIGAIMPTDMSGYVKVTYRTSAAGNTNTEVFPVKLAVDLTQSSSEPIVGG
                     SCSFTLGGTRFIDRQGSLITNIDPATGSGLTSGSINYSTGMASLTVLPVGATN"
     gene            complement(2394..2801)
                     /locus_tag="PLB25_08060"
     CDS             complement(2394..2801)
                     /locus_tag="PLB25_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41565.1"
                     /translation="MELKNLFSGATLTLPDDLLWSDEHGWSPVVSSVSYLITGSLLVQ
                     SATRQAGRAITLVGAADMAWVARSVVNVLRDWAALPLDAVSGRFELTLTDARVFTVAF
                     RHADGALEAEPVTGFPARSEADFYRITLKLMQI"
     gene            complement(2814..6830)
                     /locus_tag="PLB25_08065"
     CDS             complement(2814..6830)
                     /locus_tag="PLB25_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HOE41566.1"
                     /translation="MANNRIAVLVALEGADDGLKRALNSAQQSLGELATTAKTAGDKA
                     ARGMAEVKAGMSAFGDQVATAKTQLLAFLSINWAAGKVQEIVQVADAWNMMGARLKLA
                     TAGQNEFVIAQKALFDIAQRIGVPIQEVSTLYGKLQQAVRMLGGEQKDALTITESISQ
                     ALRLSGASATEAQSSLLQFGQALASGVLRGEEFNSVVENSPRLAQALADGLNVPIGRL
                     RKLAEEGRLTADVVVNALMSQKDKLAAEYSQLPATVSQAFQRLQNAFGQWVAQVDAAT
                     GITKKLADGLTWLATNLDTVMQWLKKIAEVGLAVLIYRLLPALVTAWQTAGAAAITAA
                     TATSAAWATANLSVTAAIASVGLLKTAFAVLGAFAVGWEIGTWLSEKFEIVRKAGIFM
                     VEILVKAVEQLQYRWEAFAAIFTSDTIDAATKRHEARLAEMNVIFAQMYADATKGSDT
                     AKAAMTTAATTAEEIAKKLEAVRQGTQEAVGRGVEAVHSAVEKLKSRLGEVEQAVTKA
                     NGVVTDATAKMAEAYKGLTAMVEANLQKQVDAVKTRYQQEQTALELSSASQATQISKS
                     TLLLTDALTQQTTLRQKATTDTLRLIDDESSARVAAAAKQGATEAERSANVTRVENEI
                     LATKRQSMVTAATEYRAHIDALNAEANRHLAEIQRIEEAKRQLTMTTEEKIRELRRQS
                     MTEFEATEDRKRQIVELQSKARDALAAGEFEQAKQLAQKAMDLAVQVGSAQTAEAKKA
                     EEAKKASEQAHTQVVTLESQAREASRKQEYDKAADLMRQADTLRAELAQKTKESDAAI
                     TQGKDGVNRSIQAIRESEDILVKSLDAQAQAHKQAAQAAVAAREQIKQTLTDTETQID
                     QITAKLKDGLKVTLDADTSRFDKAMAELDKALAEKERLLVIKADLEQAQKKLQEYEAL
                     LKEGKTLPVDADVTQAKAALDKLTVYAKQNSLIELQVTSEKAQAAINNVEGMINALGR
                     IRTESQHSVNTNADSARSEISSLNGMNTSSTHTIYVTKVETNATGGLVGAGVPHFAVG
                     GAVASPVAQAFTRMTGGSVPGSGDQDTVPRTLDAGAYVLRKAAVRKYGSNALSKIANG
                     VARFATGGSVVSPGSNVIKRNKDAAEAQQMIELGMNGLRQYVQWMRMQYGASLSIGFE
                     YDTLQGYGQLANTDRRTLESLVNRKQLTGNEKQKLDAIKANWRQAMAQPLLYGKDLER
                     DLMEYMEQHQGEFYRGGGVAKSDTVPAMLTPGEYVVNRSAVSRFGTGFFESLNNLSLP
                     AKALAARVQGFASGGLVQSLASPMTVPRPAFAGETAPVRTVRVELAAGNRSVSATVDA
                     RDETRLLDILKQAKARAF"
     gene            complement(6853..7497)
                     /locus_tag="PLB25_08070"
     CDS             complement(6853..7497)
                     /locus_tag="PLB25_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6441 family protein"
                     /protein_id="HOE41567.1"
                     /translation="MKISIRIDSAAAQAQLRRWGGEFRDKVKKAVAKAMAKEAVEIKS
                     DVRDQVASQLTVVKKTFLKGFSAYVIDKDPSRMPALYVGSRIPWVGMHEKGGTISAKM
                     LIPLHGRVGRKRFKAQIAELMRGGNAYFIKNAKGNVVLMAENIKEHDRPLAGFKRRYR
                     KAEGIKRLKRGADIPIAVLVPRVMLKKRLDIERLVVRRIPRLAASIEQQIRTVG"
     gene            complement(7494..7673)
                     /locus_tag="PLB25_08075"
     CDS             complement(7494..7673)
                     /locus_tag="PLB25_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014617598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41568.1"
                     /translation="MPDILDYTLAQLKGFAAAISRLDSARDAQILSLIAIGSRGDSKN
                     LDQTLERLTTASTSS"
     gene            complement(7691..8086)
                     /locus_tag="PLB25_08080"
     CDS             complement(7691..8086)
                     /locus_tag="PLB25_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013211018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41569.1"
                     /translation="MTDLEKLIPQDTLVQVAGETIAISPLKVGQLPAFLRVISPVMAQ
                     LSQPQINWLALFGERGDDLLTAIGIAVKKPREWVDDLAADDALLLAAKVMEVNADFFT
                     RTVIPKLDGLFSLGKGIQAANSGSTSPSA"
BASE COUNT         1554 a         2437 c         2369 g         1885 t
ORIGIN      
        1 attcgtcgca cccactggca gcaccgtcag gctggccatg ccggtcgaat agttgatgct
       61 gcccgaggtc agaccggagc ctgttgccgg atcgatgttg gtgatcaggg agccctggcg
      121 gtcaatgaaa cgtgtgccgc ccaacgtgaa gctgcacgac ccgcccacaa tgggctcgct
      181 tgagctttgc gtcagatcga ccgcaagttt caccggaaac acctcggtgt tggtgttgcc
      241 agcggcgctg gttcggtaag taaccttgac atacccggac atgtcggtgg gcatgatggc
      301 cccgatggtc tggtagatga agccaccaaa caccgttttc tcagtggcca gggtgccggt
      361 gtaccaagcg ttgtcaatca cctgggtgcc gatgaccacc tttgtccagt tgggcttggg
      421 caatgcaatc acggtatctg gcttgaaact cagggtgcca gctgcgtaat tgatcacgcc
      481 tgcacactca ggacgggtct gaaatccgcc agccccgttg tcatggacga tgacgatggg
      541 gtcaatgcgg ggtttgtagg tcagagagag ccaacgggtt gaaatctggc cctcaatggt
      601 caggtctttc tcgtcataca cggtgttcca ctcaaccttg acggtgcgcg ccagcacatt
      661 ggggttggcc agcacaatct gaatgtggcc gtcgggtgcg aaacgctcgg gggcctggaa
      721 attctcttca atgggtgggc cccacgagta ttcaatgctg acctgcgcgc cagagctggg
      781 caggatcagt ggtttaaacg tgaccacgcc atcgagatag tccactttgc cggtggcgtc
      841 accggtgagc agcccctggc cgttgtcagt ggcagtttta tccacgccgc ccgtcaccca
      901 agtcaaagtg accgtgcccg gtgccacacc agggttagcc agcgtaaata cggattgggc
      961 tgcaatggca acgccataga cagccgtgtc ggcagtggcc agcccataag tggcgagcac
     1021 ctgcgttccc gcatcgggca aggccgcgag cgtgaccatc atcgagcccg tgttgaaatt
     1081 gagcgtgcca gcgcccaccg agccaaaggc gctgcgcaac tggccgttgc ccgcatcgcg
     1141 caatgtgtac cagcggccct gagcgcggta actgagcgca aagctgccgg gcttgggtgc
     1201 aggatcaaaa atcgccacca gggtgctgga gcgtgattcg gctgtgaccg cccagctggc
     1261 ggtgtgcaag gaacgcaccg gtgttgctgc cggtgtgtag atgatcgttt tggctccagc
     1321 gtagctcacg gatggatcgg tgatcgaaat cagcccattg gcgtaatcca ctgcacccac
     1381 tgcgctgcca gcagccgaca acaagccacc atcgtcaacc agggtcaagc tgccggaggt
     1441 cagtttgagg gaattgggca tgatggcctg acccacgctg atggtgtgag aggtgttgag
     1501 tgccaccgac gtagtgagcg tcaccggccc acccgcagca gataaaacca cctgttcacc
     1561 gttgggtttc aaatcgacca gtggggtttc agtctgggca ctgggcacca gttgggtgta
     1621 gacgctggtg gcgcgaatgc tgaacgttcc aagattggcg gcagcggcca gcggcaccac
     1681 acccacatag gagccagcat cagccaccgt cgtgtctctc accttggtcg cacccgctgc
     1741 cggtgcagcc ttggcgttgc cttccgaccc ctggaaatcg gtgcgcagtg catcactgat
     1801 ggaacaggtc accacgttca tgtcgaccat ttttccctgg ctgtcctcaa attttcgttt
     1861 gactgcgctg acctcagtgg ttcggatgta ctggtttctc tcgttggttg caccctcgtt
     1921 ttgcaccagc accatggtct ggccaatccg aggcagttca gcatccacac cctgcaagat
     1981 ttgcacggca cgctggccag caatgtggtt ctccagcagc atgccgcccc acatcgggcc
     2041 cttggtgaga taactctcca ccctggccga tgccgcgtca cgccgatcaa aggtgtcacg
     2101 ggtgctgaac agcgtgacac tgacattggg atccgctgga ggctcggaga cgatgatgtt
     2161 cgagcccatg taggtgtcgg tgtccatcgt ctgcactgag acgtggagtt ttcgcaggtt
     2221 cacccggcca ccggcgcggt cgagttcaga aatatccgga aagatggcgt ttgatgttcc
     2281 gtccgtgatg actgtggctg tgggtgcgcc accaccctcg ggcacgtcgt ccatgacttg
     2341 ggatgcgacc agtttgatgt cgccagtgag aatgggcatg aaggctccag aatttagatt
     2401 tgcatcagct ttaaggtgat gcggtagaag tcagcctctg accgtgcggg aaacccggta
     2461 acgggctcgg cttcaagggc tccatcggca tgacgaaatg ccactgtgaa cacccgggca
     2521 tctgtgagcg tcaactcaaa gcggccactg accgcgtcca acggcagtgc tgcccagtcg
     2581 cgcagaacat tgacaaccga tctcgcgacc caggccatgt cggcagcgcc caccagggtg
     2641 atggcccgcc ccgcttggcg ggtggccgac tgcaccagga gagaaccagt gatcaggtaa
     2701 gacacgctgg agaccaccgg actccagccg tgctcatcgc tccacagcaa atcgtcaggc
     2761 agggtcaacg tggccccact gaagaggttt ttaagttcca tgaggattcg cccttaaaaa
     2821 gcccgggctt tggcttgttt caagatgtcg agcagtcggg tttcatccct ggcatcgacc
     2881 gtggctgaca cgctgcggtt tcccgccgcc agttcaaccc gcacggtgcg caccggtgcg
     2941 gtttctcctg caaatgcagg tcttggcaca gtcatcggtg aagccagtga ctgaacaagt
     3001 ccaccactgg caaagccctg cacacgcgca gccagggctt tcgctggcag gctcaggttg
     3061 ttgagcgact caaaaaaccc ggtgccaaac cgagataccg cagaccggtt gaccacatat
     3121 tcgccgggag tgagcatcgc aggaaccgta tcggacttgg ccacaccacc accccggtaa
     3181 aactcaccct ggtgctgctc catgtactcc atgaggtctc gttcgaggtc tttgccatac
     3241 agcagaggct gcgccatggc ttgccgccag ttggccttga tggcatcgag tttttgtttt
     3301 tcattgcctg tgagttgttt gcgattgact aggctctcaa gggttctgcg gtcggtgttg
     3361 gccagttgac cgtagccctg cagggtgtcg tattcaaatc cgatgctcaa ggaagcaccg
     3421 tactgcatac gcatccactg cacgtactga cgaagaccat tcatgcccaa ctcgatcatt
     3481 tgctgggcct cggccgcatc tttgttgcgt ttgatgacgt tcgaaccagg agaaaccacc
     3541 gaaccgcctg tggcaaaccg ggccacaccg ttggcaatct ttgacagggc attgcttcca
     3601 tacttgcgca ccgctgcttt gcgcagcaca tacgcgccag cgtctagcgt gcgcggtact
     3661 gtgtcctgat caccggagcc cggcaccgaa ccaccggtca tgcgggtaaa tgcctgcgca
     3721 actggtgatg ccactgcccc accaaccgca aagtgcggca caccagcacc caccaaccca
     3781 cctgtggcat tggtttcgac cttggtcaca tagatcgtgt gcgtgctcga cgtattcatg
     3841 ccgttgaggc tggatatctc cgaacgggct gagtctgcgt tggtgttcac actgtgctgc
     3901 gactcggtgc ggatgcgacc cagtgcattg atcattccct ccacattgtt gatcgctgct
     3961 tgggcttttt cagaagtgac ttgcaactcg atcagcgagt tttgcttggc atagacggtc
     4021 agtttgtcca gcgccgcttt ggcctgggtg acatcggcat ccaccggcag ggttttgcct
     4081 tctttgagca aagcttcata ttcctggagt ttcttctggg cctgttccag atcggccttg
     4141 atgaccagca gcctttcttt ctcggccagt gccttgtcca gttcagccat ggctttgtca
     4201 aaccggctgg tgtccgcgtc cagcgtgact ttcaagccat ctttgagttt ggcggtgatc
     4261 tggtcaatct gagtttctgt gtccgtgaga gtctgtttga tctgctcacg cgctgctact
     4321 gcggcttggg ctgcctgttt gtgcgcctga gcctgagcgt ccagtgactt gacgagaatg
     4381 tcctcggatt cccgaatggc ttgaatggat cggttgacac cgtccttgcc ctgggtgatc
     4441 gccgcatcgg actcctttgt cttttgcgcc agttctgcgc gcagggtgtc tgcctgtcgc
     4501 atcaggtcgg cagctttgtc gtactcctgt ttgcgcgatg cctcacgcgc ctgcgactcc
     4561 aacgttacga cttgggtgtg cgcctgctca gatgcttttt tggcttcctc tgcctttttg
     4621 gcctcggccg tctgggcgct gcccacttgc accgccaaat ccatggcttt ctgcgccagt
     4681 tgtttggcct gctcaaactc accagcggcc agagcatcac gcgccttgct ctgcaactca
     4741 acaatctggc gtttgcggtc ttccgtcgct tcaaactccg tcatgctttg gcggcgtagc
     4801 tcacggattt tttcctccgt ggtcatcgtc agttgacgtt tggcttcttc aatgcgctgg
     4861 atttccgcca aatgccggtt ggcttcagcg ttcaaggcat cgatgtgagc gcggtattcc
     4921 gtggcggctg tgaccatcga ctggcgtttg gttgccagaa tctcattttc gacccgtgtc
     4981 acgttagcgc tgcgctcggc ttctgttgcg ccttgcttgg ccgctgctgc caccctggcg
     5041 ctggactcgt catcgatgag tctcagtgtg tctgtggtgg ccttttgacg caaggtggtc
     5101 tgctgagtca gtgcatcggt gagcagcagg gttgatttgc tgatctgggt cgcttgcgag
     5161 gcagacgata actccaatgc cgtctgctcc tgctggtacc gtgttttcac agcatcaacc
     5221 tgtttttgca ggttggcctc gaccatggca gtcaagcctt tgtacgcctc agccattttt
     5281 gctgtcgcat cggtcaccac gccgttagcc ttggtgaccg cctgttcgac ctcacccagc
     5341 cgggatttaa gcttttccac ggccgagtga acagcctcaa cgccacgacc gaccgcttct
     5401 tgcgtacctt ggcgcacggc ttctagcttt ttggcaatct cttctgctgt agtggccgca
     5461 gtggtcatgg ctgccttggc tgtgtctgaa cccttggtgg catccgcata catctgcgca
     5521 aagatcacat tcatttcagc caggcgagcc tcgtggcgct tggtagctgc atcgatggtg
     5581 tcgctggtga agatcgctgc aaacgcttcc cagcggtact gcaactgctc aactgctttg
     5641 accaggattt cgaccatgaa gatgcccgcc ttgcgcacaa tctcgaattt ctcggacaac
     5701 caggtgccga tttcccagcc gacggcgaac gcacccagca cagcgaaggc tgttttgagc
     5761 aggccaacac tggcaatcgc agccgttact gacaaattgg cggtggccca ggccgcagag
     5821 gtggcggtgg cagccgtgat ggctgcggcg ccggcagttt gccaagccgt gaccaatgct
     5881 ggcagcagcc ggtaaatgag caccgccaag cccacctcgg cgattttctt aagccactgc
     5941 atgaccgtgt ccagattggt ggcgagccaa gtcaggccat cggcgagttt cttggtgatg
     6001 cctgtggccg catccacctg cgctacccac tgcccgaagg cgttttgcag gcgctggaaa
     6061 gcctgactga ccgttgctgg caactgggag tattcggcgg caagcttgtc cttctggctc
     6121 atcaaagcgt tgaccaccac gtcagcggtg agcctgcctt cttcagccag cttgcgcagc
     6181 cgaccaatgg gcacgttcaa accatcggcc agggcttggg ccagacgtgg actgttctcg
     6241 accacggaat tgaattcctc gccgcgcagc acaccagatg ccaaggcctg gccgaactgc
     6301 aagagtgagg actgggcttc cgtggccgac gcaccggaca ggcgcagtgc ctgcgagatg
     6361 ctctcggtaa tggtcaatgc gtccttttgt tcaccaccca acatgcgcac cgcttgctgc
     6421 aacttgccat acagggtcga gacttcctga atcggcaccc caatgcgctg agcgatgtca
     6481 aacagcgcct tttgcgcgat gacaaactca ttctgaccag cggttgccag tttcaggcgc
     6541 gcgcccatca tgttccaggc atcggccact tggacgatct cttgcacttt gcctgccgcc
     6601 cagttgatcg acaggaacgc cagcagctgc gtcttggcag tggccacctg gtcaccaaac
     6661 gccgacatgc ccgctttgac ctccgccata ccgcgcgcag ccttgtcgcc agcggtcttg
     6721 gccgtggtgg ccaactcacc caggctttgc tgtgcagaat tcagggcgcg tttgagcccg
     6781 tcatcggcac cctcaagggc gactaaaacg gcaatacggt tgttggccat gggtcaattt
     6841 caaatttgag ggtcatccga ccgtgcggat ttgttgttcg atgcttgctg caagtcgtgg
     6901 gatgcgccgc accaccagac gctcaatgtc gagtcgcttt ttgagcatga cgcgcggaac
     6961 gagtaccgca atcggaatat cagcaccacg cttcaagcgt ttgattcctt cagcttttcg
     7021 gtaccggcgt ttgaatccag ccaaggggcg gtcgtgctct ttgatgttct cggccatcag
     7081 caccacgttg cccttggcgt ttttgatgaa gtaagcgttg ccgccccgca tgagttcagc
     7141 aatctgagcc ttgaagcgct tgcggccgac ccgaccatgc aagggaatca gcatcttggc
     7201 cgaaatggtc ccgccttttt catgcatgcc aacccagggg atgcgcgagc ccacgtacag
     7261 cgctggcatg cgactggggt ctttgtcgat cacgtaggcg gagaacccct tgaggaaggt
     7321 ctttttgacc accgtcagtt ggctggccac ctggtcgcgc acgtccgact taatctccac
     7381 tgcctctttc gccatggcct tggccaccgc ttttttgacc ttgtcgcgaa actccccacc
     7441 ccatcggcgt agctgggctt gcgctgcagc gctgtcgatt cggatggaga ttttcatgac
     7501 gaggttgatg cagtggtcag acgttcaagc gtttgatcaa ggtttttgga gtcaccccgg
     7561 ctaccgatgg caatcaggga cagaatttgg gcatcacgcg cgctgtccaa gcgagagatg
     7621 gcagcagcaa aacccttcaa ctgcgccagc gtgtagtcca ggatgtcggg caaccggtgg
     7681 ccgtgctcga tcaggcgctg ggtgaggtcg aaccagagtt ggctgcttga attcccttgc
     7741 ccagactgaa cagaccgtcg agtttgggaa tcaccgttcg ggtaaaaaaa tcagcgttga
     7801 cctccatcac cttagctgcc agcagcaatg cgtcgtctgc ggccagatcg tccacccact
     7861 cacgcggttt tttgactgcg atgccgatgg cggtcaacaa atcgtcgcca cgctcgccaa
     7921 acagcgccag ccagttgatt tgcggctggc tcaactgcgc catcaccggc gagatcaccc
     7981 gcaggaaagc aggcaactgg cctactttga ggggcgagat cgcaatggtt tcgcctgcca
     8041 cttgcaccag ggtgtcctgg ggaatgagct tttccaaatc agtcatgacg cgcctttcag
     8101 atttggacaa tgcggccgaa ctggcccagc gttgcatcga atggcttgct ggaatcggcc
     8161 aagagcgagc cttccatctc gaacttgttg tagtcgttgg agatgaacga gatttccttc
     8221 aagggatcaa aggctacccg gtaca
//
LOCUS       DAOSLL010000142         8210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8135_length_8210_cov_4.267443, whole genome shotgun sequence.
ACCESSION   DAOSLL010000142 DAOSLL010000000
VERSION     DAOSLL010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8210
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8135_length_8210_cov_4.267443"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..957
                     /gene="cheB"
                     /locus_tag="PLB25_08085"
     CDS             <1..957
                     /gene="cheB"
                     /locus_tag="PLB25_08085"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HOE41570.1"
                     /translation="TLDINMPEMDGITALSLIMTERPVPVVMVSSLTEKGALATFEAL
                     NLGAVDYVAKPGGTISLTLDQVAPDLLAKVRAAARAKLGRGRAAPVAKTVAKPAGSAQ
                     GLRQRLIDERESAQRAATLAPAVGSEGLLIIGVSTGGPRALEQVLPALPANFPWPVLV
                     AQHMPSSFTGPFSARLDGLCELSVVEAARPMPVQVGTIYIGKGGADMVLTRRAGVLTV
                     LPKPESSEHLWHPSVELLARSALEHVKPGNLVAVMLTGMGFDGADGFAAIKRAGGRTI
                     AESEETAVVFGMPAVLIEKGGASLVVPLDKVATQVKAWASRG"
     gene            982..1602
                     /locus_tag="PLB25_08090"
     CDS             982..1602
                     /locus_tag="PLB25_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HOE41571.1"
                     /translation="MPLIKPQRSPDAAPARELERDCASLVISLNNPSPVTRRWAARDL
                     ADCPGSSGALVARLGEESDASVREVILTTLTRLGDAAAVAGLVQCMRSDDAALRNEAI
                     EAMKLLPDAVAPIMRDLLADPDSDLRIFAVNILESLKHPDVELWLREVIERDPHINVC
                     ATAVDLLGEVGSAAALDSLYQLKARFAGQAYIVFAADLAIKRIQQD"
     gene            1611..2456
                     /locus_tag="PLB25_08095"
     CDS             1611..2456
                     /locus_tag="PLB25_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOE41572.1"
                     /translation="MPAPSISEEDFHKFREYFYRKTGIQYEVSKRYFVDKRLLERMAA
                     TDNALFRSYFTMLRFQASGEELQVLTNLMTVNETYFFREEYQFKCLVDSIMPHLVEKK
                     RDQTTLRIWVIPSSSGEEPYSIAIYLLEHWADIHNYDVEIVASDIDTTILTQARRGLY
                     SDRSIGQLPLPLLQKYFRKSRDGYQISDELRGAVEFTRVNLSEPADTRPYRNFDVVFC
                     RNLLIYFDDLSRKTAAETFYDALKPGGFICLGHSESMSRISSLYKVVKFPQAIVYQKP
                     TERQP"
     gene            2453..2824
                     /locus_tag="PLB25_08100"
     CDS             2453..2824
                     /locus_tag="PLB25_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE41573.1"
                     /translation="MKRILIIDDAATVRMYHRNILQTAGYAVDEAINGMEALEKALQT
                     RFDLYLVDVNMPKLDGYGFLRELRSQDVAQAPAIMISTEASADDRKRAYAAGANLYLV
                     KPMQPEQLLAPVALLLGDMTP"
     gene            2821..5592
                     /locus_tag="PLB25_08105"
     CDS             2821..5592
                     /locus_tag="PLB25_08105"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOE41574.1"
                     /translation="MNALLEQFLSESREALQGIGEKLMQLEDAPEDQELMTQLFRLVH
                     TLKGNSGLFEFPEMTRVLHAGEDLMDAVRNERVHYSQALADHLLDAMDFVGMLCDDIE
                     AERPMPAGLAADAARLARSLRALIGSTDPAHPDPAVDTKNIAGDATAASAGGCFHTDP
                     TALARIPEAIRLTAWQQAQAGSPLFWVHYQPSEECFFQGEDPLHQAKLTPGLLWGGML
                     ARAPWPKLAELDAYRCMLDFDLLAAASRDDIEQHYRYVPDQIQILEVPAQALALPHGD
                     PNGGPAYEDFVADALGFLERGDLTGLGRAVRSMLELSNSALWLSSDLRWISAFLEASP
                     VNLPAVRTLIESLRTLSVPNWAVALANQAQVAPFSAANQAPDLASTVPAPPATALAHA
                     PVNLQAQAIADVLAVQREVLALPDDAGWLGGRIKAAQASLLGCLRASGRTAEEATLNT
                     AAAAALLANTAAPLAQWLAQAFVPEGVSARTKTTQTTPASAPMPEPATAPDPHSQPVP
                     AKDAVVAESPELDLRMNRRADDANSAKTLKVDQVKVDRLMNLIGEMVVAKNAIPYLAS
                     RAESVFGVRELSREIKAQYAVINRISEEMQDAIMQVRMMPVSFVFQRFPRLVRDISRK
                     LGKEVNLVLEGQETAADKNIIESLGDPLIHIVRNSLDHGLEMPDVRVALGKPALGTLK
                     IVAKQEADRVVIEISDDGKGIDPAVIKQKAYQKGLIDEAMLDRITDQEAINLVFAAGF
                     STAEVVSDLSGRGVGMDVVRTAVERVNGSIALDSVKGQGTVLRLSLPLSMAVTNVMTI
                     ASNGQMFGVPMDMVVETVRVPSANVRLIKQRQATVLRGRIVPLLSLNALLGLAAPPLT
                     NDDDELATLVVRVGEDNVGLLVDEFHGTADIILKPLLGVLSSLRAYSGSALMGDGSVL
                     MVINLKELL"
     gene            5592..5834
                     /locus_tag="PLB25_08110"
     CDS             5592..5834
                     /locus_tag="PLB25_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41575.1"
                     /translation="MGLEFRKKIALLSDAVGVEDAEKLLDWLQGKPAARVDLSACTHL
                     HPANIQVLMAAGAPICAWPSDAALADWLKPALVDVQ"
     gene            5856..6233
                     /locus_tag="PLB25_08115"
     CDS             5856..6233
                     /locus_tag="PLB25_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010398075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE41576.1"
                     /translation="MAKKTILIVDDSATMTLSVKSSLEMNGFTVQTASDGVQAMIKLK
                     AGLKPDLIITDINMPNMGGLELIKNVKALPGYRFVPILTLTTESDSKKRDEGKKLGAT
                     GWLVKPVSGVDLVKVIKQVVPGA"
     gene            6270..7511
                     /locus_tag="PLB25_08120"
     CDS             6270..7511
                     /locus_tag="PLB25_08120"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE41577.1"
                     /translation="MLKSQPKPFPSVTGLAAIAVTSSLMLWLAPWAHEQFMPSWHVDL
                     RVEMAVVLCVAFGGSSILTLLFKWLAKKKTFDDEWLMLAKHMPREQVVTEVREVAPFL
                     DVMSKQLDGAVKETEHGVMGLINSLNTIHEVSQHQLQRIKASEESGIELATALRDKVM
                     VDKQLGQILRMFIDKQQQDVEINLGRIKRLQEVKSLTTLVADISQVAKQTNILAVNAA
                     VEAARAGDAGRGFAVLAAEIRELSNRTAGAAVSISLKIKTATEGIDEELLNATEVQER
                     NSATGSMRKVIADVDGMQHRFAAASQSLQEIIEGVKAGHQDIVTGLSEALGQVQFQDV
                     IRQRVAQVQKAQEELNDHLQSMADQLIDKPWDPQTMVTLRQRLDDQMASYVMQSQRET
                     HQHVVGKTTPLADARPNIELF"
     gene            7517..>8210
                     /locus_tag="PLB25_08125"
     CDS             7517..>8210
                     /locus_tag="PLB25_08125"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HOE41578.1"
                     /translation="MARTLVISNRKGGSGKSSTAVNVAAELAAQGQRVLLVDLDTQGH
                     CAVGLGVKPQRDMPTVHGFLAGRHDLRQAVVASNWPGLHLIPADPLFEHGGVGGNEAM
                     LRQALLAEGLLADYDTVVLDTPPSLDGLLINALCAADRVLVPFVPHYLSGEGVRQLAR
                     LLFRVASRGLNEQLKVLGFLPVMVDLRIGQHREVNAGLSRQFGAARLLPGIRTDIKVA
                     EAFAVGKPVREHA"
BASE COUNT         1620 a         2416 c         2591 g         1583 t
ORIGIN      
        1 acgctcgaca tcaacatgcc cgagatggac ggcattaccg ccttgtcgct gatcatgacc
       61 gagcgaccgg tgccggtggt gatggtatcg tcgctcaccg aaaaaggcgc tctggccact
      121 tttgaggcgc tgaacttggg cgcggtggac tacgtggcca agcctggcgg caccatttcg
      181 ctcacactgg atcaggttgc gccggatttg ttggcaaaag tgcgcgccgc cgcccgtgcc
      241 aaactgggcc ggggccgggc tgcacccgtt gccaagaccg tggcgaagcc cgccggttca
      301 gcgcagggct tgcgtcagcg cctgatcgac gagcgcgaaa gtgcgcagcg cgccgcaacc
      361 ttagcgcctg ccgtcggcag tgaggggctg ctgatcattg gtgtgtccac tggcgggccg
      421 cgtgcgctgg agcaagtgct gcctgccttg ccggccaatt tcccgtggcc ggtgctggtg
      481 gcgcagcaca tgcccagcag ttttaccggg cctttttcag cccggctcga tggtttgtgt
      541 gaactctcgg tggttgaagc cgcgcgcccc atgccggtgc aggttggcac gatttacatc
      601 ggcaagggcg gggccgacat ggtactgacc cgccgggctg gtgtgctgac cgttttgcca
      661 aagcccgagt cgtcagagca cctgtggcac ccctcggtgg agttgctggc acgcagcgcg
      721 ctggagcacg tgaaacctgg caatctggtg gcggtgatgc tgaccggcat gggttttgac
      781 ggcgccgacg gctttgccgc catcaaaaga gccggtggcc gcaccattgc cgagtcggaa
      841 gaaacagcgg tggtgtttgg catgccggct gtgttgattg aaaaaggcgg ggcctcgctg
      901 gtggtgccgc tggacaaagt ggcaacccag gtcaaggcct gggcctcacg tggctaagtt
      961 ggacggataa ggagtttcac catgcccttg atcaagcccc agcgttcacc cgatgccgcc
     1021 ccagcgcgcg agctcgagcg tgactgtgcc agcctggtca tcagcctgaa caaccccagc
     1081 ccggtcaccc ggcgctgggc agcgcgcgac ctggcggact gtccgggcag ctcaggcgcg
     1141 ctggtggcac ggctgggtga agaatctgat gcttccgtgc gtgaagtcat tttgacgacg
     1201 ctgacccgtt tgggtgacgc tgccgccgtg gccggcctgg ttcagtgcat gcgcagcgat
     1261 gatgccgcgc tgcgcaacga agccatcgag gcgatgaagc tcttgcccga cgcggtagca
     1321 cccatcatgc gtgacctgct ggccgacccc gacagcgacc tgcgcatttt tgcggtgaac
     1381 attctggagt cgctcaaaca ccccgatgtg gagctttggc tgcgcgaggt gatcgaacgc
     1441 gacccgcaca tcaacgtctg tgccacggcg gttgacctgc tgggcgaggt tggctcagcg
     1501 gccgcgctgg actcgctgta ccagcttaaa gcccgctttg ccggccaggc ctatatcgtg
     1561 tttgcggcgg atctggcgat caaacgaatc cagcaagact gaaacccttt atgcccgctc
     1621 ccagcatctc cgaggaagac ttccacaagt tccgggagta cttctaccgc aagaccggca
     1681 ttcagtatga ggtctcgaag cgctactttg ttgacaagcg gctgctcgag cgcatggccg
     1741 ccaccgacaa cgccttgttc agaagctact tcaccatgct gcgctttcag gcctctggcg
     1801 aagagttgca ggtgctgacc aacctgatga cggtcaacga aacctatttt tttcgtgagg
     1861 aataccagtt caagtgcctt gttgactcga tcatgccgca tctggtggag aagaagcgcg
     1921 accagaccac cttgcgcatc tgggtgattc cgtcgtcctc gggcgaggag ccgtattcga
     1981 ttgccattta cctgctggag cactgggctg atattcacaa ctatgacgtg gaaattgtcg
     2041 cctccgacat tgacaccacg atcttgaccc aggccaggcg cggcctgtac tccgaccgct
     2101 cgatcgggca attgcccctg ccgcttttgc aaaagtactt tcgcaagtcc cgcgacggtt
     2161 accagatcag cgacgaactg cggggtgcgg tggaattcac ccgggtcaac ctgtccgaac
     2221 ccgccgacac ccgaccttac cgaaactttg acgtggtgtt ttgccgcaac ctgctgattt
     2281 attttgacga cctgtcgcgc aagaccgctg ccgagacttt ttacgacgca ctcaagcccg
     2341 gcggcttcat atgcctgggg cattcagagt cgatgagccg catttcttcg ctgtacaagg
     2401 tggtcaagtt cccgcaggcc atcgtctacc agaagcctac ggagcgacaa ccatgaaacg
     2461 gatattgatc attgacgacg cggccactgt gcgcatgtac caccgcaata tcttgcaaac
     2521 ggctggctac gcagtcgacg aggccatcaa tggcatggag gcgttggaga aggcgctgca
     2581 aacccggttt gacttgtacc tggtggatgt caatatgccc aaacttgacg gttatggttt
     2641 tttgcgcgag ctgcgcagcc aggacgtggc gcaggccccg gccatcatga tctctaccga
     2701 ggcatcggcc gatgaccgca agcgcgccta tgcggcggga gccaacttgt acctggtcaa
     2761 gccgatgcag ccagagcaac tgctggcccc ggttgccctg ctgctggggg acatgacacc
     2821 atgaatgcct tgctggagca atttctaagc gagtcgcgcg aggcactgca aggcatcggc
     2881 gaaaagctga tgcaactcga agacgcgccc gaagatcagg agctgatgac gcaactgttc
     2941 cgtctggtgc acacgctcaa gggcaacagc ggcctgtttg aatttcccga gatgacccgc
     3001 gtgctgcacg ccggcgaaga cctgatggac gcggtgcgaa atgagcgggt gcattactcg
     3061 caggcgctgg ccgatcatct gctcgatgcc atggacttcg tcggcatgct gtgcgacgac
     3121 attgaggccg agcggccgat gcccgcaggc ctggcagccg atgccgcccg gctggcgcgc
     3181 agcctgcgcg ccttgatcgg cagcactgac ccggcccatc ccgaccctgc agttgataca
     3241 aaaaacatag ctggtgacgc aacagcagca agcgctggcg gctgttttca taccgatccc
     3301 acggccctgg cccgcatccc ggaagcgatt cgcctgaccg cctggcaaca ggcccaggcg
     3361 ggtagcccct tgttctgggt gcattaccag ccgtctgaag agtgcttttt tcagggtgaa
     3421 gacccgctgc accaggccaa actgacaccc ggcctgctgt ggggtggcat gctggcacgc
     3481 gcgccctggc ccaagctggc agagctggat gcctaccgct gcatgcttga ctttgacctg
     3541 ctggctgctg ccagccgcga cgatattgag caacattacc gttatgtgcc tgaccagatc
     3601 cagattctgg aagtgcccgc gcaggcgctg gccttgccac acggcgaccc caatggcggc
     3661 ccggcatatg aagactttgt ggccgatgct ttgggttttc tggagcgtgg tgacctcacc
     3721 ggcctgggcc gcgcggtgcg cagcatgctg gagctttcca acagcgcgct gtggctgtcg
     3781 tctgacttgc gctggatttc agcgtttctg gaggcatcgc cagtcaacct gcccgcagta
     3841 cgcacgctga tcgagtcgct gcgtacactt tcagtgccaa attgggctgt agcccttgcc
     3901 aatcaagctc aggtagctcc ttttagtgca gcaaatcaag cccctgatct ggcttcgact
     3961 gtgccagcgc caccagccac tgcgctggcc catgcaccag tcaacctgca ggcccaagcc
     4021 attgcggatg tgctggcggt gcagcgcgaa gtattggcct tgcccgatga tgccgggtgg
     4081 ctgggcgggc gcatcaaggc ggcgcaggcc tcgctgctgg gctgcctgcg cgccagtggt
     4141 cgcacggcag aagaagccac gctcaacacc gccgctgctg ctgcgttgct ggccaacacg
     4201 gcggccccgc tggcgcaatg gctggcacag gcgtttgtgc cagagggcgt gtcagcccgg
     4261 accaagacga cgcaaacgac accggcatcc gcgccaatgc ccgagccagc cactgcgcct
     4321 gatccccatt cgcagccggt acccgccaaa gatgcagttg tggccgagtc gcccgagttg
     4381 gacctgcgca tgaaccgccg ggccgacgat gccaatagcg ccaaaacgct caaggttgac
     4441 caggtcaagg tggaccggct gatgaatctg attggcgaaa tggtggtggc caagaacgcc
     4501 atcccctacc tcgccagccg ggccgaaagc gtctttggcg tgcgcgagct ctcaagggag
     4561 atcaaggcgc aatacgcggt gatcaaccgt atttctgaag aaatgcagga cgccatcatg
     4621 caggtgcgca tgatgccggt gtcgttcgtg ttccagcgct ttccacgtct ggtgcgcgac
     4681 atctcgcgca agctgggcaa agaagtcaac ctggtgcttg aaggccagga aaccgccgct
     4741 gacaaaaaca tcatcgaatc gctcggcgac ccgctgattc acatcgttcg caatagcctg
     4801 gaccacggac ttgagatgcc agatgtgcgt gtagcccttg gcaagcctgc gctgggcact
     4861 cttaaaattg tagctaagca agaggcagac cgggtcgtga tcgagatcag cgacgacggc
     4921 aaaggcattg acccggcagt catcaagcaa aaagcctatc agaaaggcct gattgacgag
     4981 gccatgctgg atcgcatcac cgaccaggag gccatcaacc tggtgtttgc tgcgggcttt
     5041 tccaccgcag aggtggtgtc agacctgtcg ggccggggcg ttggcatgga cgtggtgcgc
     5101 accgccgtgg agcgcgtcaa cggcagcatt gcgcttgaca gcgtcaaggg ccagggcacg
     5161 gtgctacgcc tgtccttgcc gctgtcaatg gcggtcacca atgtgatgac gattgcgtcg
     5221 aacggtcaga tgtttggcgt gccgatggac atggtggtgg aaaccgtgcg tgtgcccagc
     5281 gccaacgtgc gcctgatcaa gcagcgccag gctaccgtgc tgcgcgggcg cattgtgccg
     5341 ctgctgtcgc tcaatgcttt gctggggctg gcagcacccc cattgaccaa cgacgacgat
     5401 gaactggcca cgctggtggt acgtgtgggc gaagacaacg taggcctgct ggtcgatgaa
     5461 tttcacggca cggccgacat catcctcaag ccgctgctgg gtgtgctgag cagcttgcgg
     5521 gcctattcag gctcggcgct gatgggcgac ggctcggtac tgatggtgat taacctcaag
     5581 gagctgctct gatgggtctg gaatttcgca agaagatcgc gttgctgagc gacgcggtgg
     5641 gcgtggaaga cgctgaaaag ctgctggact ggctgcaagg caaaccggcc gccagggtgg
     5701 acttgtcggc ctgcacgcac ctgcacccgg ccaatatcca ggtgctgatg gccgctggcg
     5761 cgccaatctg cgcctggccc agcgacgcag cgctggccga ctggctcaag ccagcgctgg
     5821 tggacgttca atagcatgaa agagagaaca tctgtatggc aaaaaaaaca attttgatcg
     5881 tggacgattc ggcgacgatg acgctgagcg tcaaatcgag cctggagatg aacggtttta
     5941 ccgttcaaac cgccagtgat ggtgtgcagg ccatgatcaa gctcaaggcc gggcttaaac
     6001 ccgacctgat cattaccgac atcaacatgc ccaatatggg cgggttggaa ttgatcaaaa
     6061 acgtcaaggc actgccgggc taccgctttg tgccgatcct gacgctgacc accgagagcg
     6121 attccaaaaa gcgcgacgaa ggcaagaagc ttggcgccac cggctggctg gtcaagccgg
     6181 tgtctggggt ggatctggtc aaggtcatca agcaggtggt accgggtgca tgacttggtg
     6241 gctaacgtga acttgttctg gagacggtga tgttgaagtc acaacccaaa ccctttcctt
     6301 ccgtcacggg actggctgct attgcggtga catcttcgct gatgctttgg ctggcaccgt
     6361 gggcgcatga acaattcatg ccttcgtggc atgttgattt gcgggtggag atggcggtgg
     6421 tcctgtgtgt cgcgtttggt ggctcgtcga tcttgacgct gctgttcaag tggctggcca
     6481 aaaaaaagac gtttgacgac gaatggctga tgctggccaa acacatgcca cgcgaacaag
     6541 tggtgaccga ggtgcgcgag gtggcccctt ttctggatgt catgagcaag cagcttgatg
     6601 gcgcagtcaa ggaaaccgag cacggtgtca tgggcttgat caactcgctc aacaccatcc
     6661 acgaggtttc tcaacaccaa ttgcagcgca tcaaagcgtc ggaagaaagt ggcatcgaac
     6721 tggccactgc gttgcgcgac aaggttatgg tggacaagca gctcgggcag attctgcgaa
     6781 tgttcatcga caagcagcag caggacgttg agatcaacct cgggcgcatc aagcgcctgc
     6841 aagaggtcaa gagcctgacc acgctggtgg ctgatatttc gcaagtggcc aagcaaacca
     6901 atatcctggc cgtcaatgcg gcggtggagg cggcacgcgc cggcgatgcc ggacgtggct
     6961 ttgccgtgct ggcggcagaa atccgcgagc tgtcgaaccg caccgccggg gctgctgtga
     7021 gtatttcgct caagatcaag acagccactg aaggcattga cgaagaactg ctcaatgcca
     7081 ccgaggttca agagcgcaat tcagccaccg gcagcatgcg caaggtgatt gcggatgtgg
     7141 atggcatgca gcacaggttt gcagccgcat cgcaaagcct gcaggagatc atcgagggtg
     7201 tcaaggccgg gcatcaggac atcgtgacgg gtttgtccga ggcgctgggg caagttcagt
     7261 tccaggacgt gattcgccag cgggtggcgc aggtgcaaaa ggcacaggaa gagctcaacg
     7321 accatctgca aagcatggcc gaccaactca tcgacaagcc ctgggacccg caaaccatgg
     7381 tcacgctgcg ccagcgtctg gatgaccaaa tggcgagcta tgtgatgcaa agccagcgcg
     7441 agacgcatca gcatgtggtc ggcaagacga caccattggc cgatgcgcgt cccaacatcg
     7501 agctgttttg aacgccatgg cacgcaccct ggtcatcagc aaccgcaagg gcggctcggg
     7561 caaatcatcc actgcggtga atgtggccgc tgaactggca gcgcagggcc agcgcgtgct
     7621 gttggtggac ctcgacaccc agggccattg cgccgtggga ttgggcgtca agccgcaacg
     7681 cgacatgccc acggtgcatg gctttctggc tggccgccat gacttgcgcc aggcggttgt
     7741 ggcatcaaac tggccggggc tgcatttgat cccggccgac ccgttgtttg agcatggcgg
     7801 cgtcggcggc aacgaggcca tgctgcgcca ggccctgctg gccgagggct tgctggcaga
     7861 ctacgacacc gtcgtgcttg acaccccgcc atcactcgac ggcctgctga tcaacgcgct
     7921 gtgcgccgcc gaccgggtgc tggtgccgtt tgtgccgcat tacctgtctg gcgaaggtgt
     7981 gaggcaactg gcgcgactgc tgtttcgggt ggccagccgg ggtttgaatg agcagctcaa
     8041 ggtgctgggc tttttgccgg tgatggtgga cctgcgaatt ggccagcacc gcgaggtcaa
     8101 cgccggcctg tcgcgccagt ttggcgcggc gcgcctgctg ccgggcattc gcaccgacat
     8161 caaggtggct gaagcctttg ccgtgggcaa accggtacgc gagcacgcgc
//
LOCUS       DAOSLL010000143         8174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8181_length_8174_cov_5.070822, whole genome shotgun sequence.
ACCESSION   DAOSLL010000143 DAOSLL010000000
VERSION     DAOSLL010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8174
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8181_length_8174_cov_5.070822"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /gene="ppnK"
                     /locus_tag="PLB25_08130"
     CDS             <1..583
                     /gene="ppnK"
                     /locus_tag="PLB25_08130"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543357.1"
                     /note="catalyzes the phosphorylation of NAD to NADP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HOE41579.1"
                     /translation="ITDIPYDSFTASLGKMLQGEYVEDHRSLMKACVIRGGHCVFKAM
                     AMNDVVVNRGATAGMVELRVEVDGHFVANQRADGLIIATPTGSTAYALSSGGPLLHPS
                     IPGWVMVPIAPHTLSNRPIVLANTSHIAIEIASGRDASASFDMQSLASLMHGDRIEVT
                     RSAHHVRFLHPRGWSYFDTLREKLHWNEGVATT"
     gene            580..2238
                     /gene="recN"
                     /locus_tag="PLB25_08135"
     CDS             580..2238
                     /gene="recN"
                     /locus_tag="PLB25_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HOE41580.1"
                     /translation="MSLKRIVLRDFVIVSELELELHSGFTVLTGETGAGKSILIDALQ
                     LVTGGRADLTMIREGASRTDISAEFDATPGIACWLQEAGFETDAASGLLLRRTVDVQG
                     KSRAWINGSPATATQLRAVGEQLLDIHGQHAWQSLTRADAVRGLLDAYAKVSTTQVAV
                     LWQQWHTAQERLTQARASQESLLQERERLTWQIGELEKLAPLPHEWPTLNAEHSRLSH
                     AQALLDAAQMALAVLSEDDDNAIGRIGTAHDLLQKQELIEPVFKGLTEVLSSSLAQLE
                     DTVHTLRAYLRKTELDPDRLSTLDDRLGLWVSLARRYKRVPEELPELLNHWKQALLVL
                     DAAADLQQLEQALASAWQAYLREAQALTQVRNGAAVQLAKTISNAMQGLGMQGGQFEV
                     QLQPAAQPLQSGLEDVSFLVAGHAGSTPRAVNKVASGGELSRIALAIAVTTSQLGTAQ
                     TLIFDEVDAGVGGAVAETVGKLMHQLGVDRQVLAVTHLPQVAACADQHLVVSKKLQGG
                     CTISSVASVKAEERVTELARMLGGERILATTLAHARDLLLTQEP"
     gene            2240..3130
                     /gene="rapZ"
                     /locus_tag="PLB25_08140"
     CDS             2240..3130
                     /gene="rapZ"
                     /locus_tag="PLB25_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HOE41581.1"
                     /translation="MEASPQGLQIVLITGMSGSGKSIALHALEDLGFYCVDNLPPELF
                     LPLVELEHANHASRLAIAMDVRSAGSLPLLPAQFRQLKAAGFQFDSIFLDASTDTLIR
                     RFSETRRRHPLSQGSHSGHVLDQRHALAEAIELERELLSDLRSQAHVIDSSMIRPTQL
                     QSYVTAFIEAPGEQLTLVFESFAFKRGIPSDADYVFDVRMLPNPHYEPALRALTGMDA
                     PVIDFLRQEQDVQRMQDDVRAFLTHWLEALARNHRSYVTVAIGCTGGQHRSVYLVEQL
                     AASFAARWVTLKRHREMDAR"
     gene            complement(3127..4194)
                     /gene="mutY"
                     /locus_tag="PLB25_08145"
     CDS             complement(3127..4194)
                     /gene="mutY"
                     /locus_tag="PLB25_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HOE41582.1"
                     /translation="MPLPEPWSGGFSLSGALLRWQRQHGRQDLPWQNTQDPYRVWLSE
                     IMLQQTQVVTVGEYFQRFLAQFPDVRALAEASLDSVLALWAGLGYYSRARNLHRCAQD
                     VMRLHGGAFPRSASQLQTLPGIGPSTAAAIASLCFAERVAILDGNVKRVLTRFLGFDA
                     DLAQAANLRRLWQLANELLPQDALATDMPRYTQGIMDLGATVCTAKKPDCDVCPLQAH
                     CQARARANPGKFPVNTRKLKRSAQSIWMLWAQADDGALWLDQRPTPGVWAGLYCFALF
                     NDRAELLLAVPGILSAQLRDESAFKHVLTHKDLYLHPVRVLLPRTCQPVARGAWIDAH
                     QWPQLGLPKPIRKLLNGEPGR"
     gene            complement(4181..6142)
                     /locus_tag="PLB25_08150"
     CDS             complement(4181..6142)
                     /locus_tag="PLB25_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HOE41583.1"
                     /translation="MGTSFNEKFDQHGVWRREFALRLKLLAQWLKDQDMLDASVEDRL
                     HRLEAQIRVDKVMVAFVAEFSRGKSELINAVFFADYGQRIMPASAGRTTMCPTEMGYD
                     PNVPPCLRLLPIETRLQPQSLMEWRMVPEKWERTELDVNNPKQLAEAFAKVAETRRVT
                     LDEARALGFWQHLTAQHNAAPDGGALVDVPRWRHALINIGHPLLKQGLIILDTPGLNA
                     IGAEPELTVSLIPQAQAVVFILGADTGVTQSDLSIWREHLVDDDQDVTTRLVVLNKID
                     TLWDSLDSPQEVQAQIDRQRRDSARILGISEQQVVAISAQKGLVGKVTNDAALLTRSC
                     LGDLEDALSRGIMGRRQKILGAAVARGIAALRSETGRVVHIRRRDLTEQLIELKGLRG
                     KNRPVIKNMRSRIAQEQAEFDAAGVKIHAVRSVHLKLLREIFNLLGPVNLKREMAQLT
                     TSLNQKGFKLGIKRSYGETFDGLRANLRLVHANGLEIHTMLVAVFLQLNTEYGFSLQV
                     PEEPQVDTYLAELDLIERSHLQYLGIGNAFRLALPEFAQQVVRALSTRLRGVYETALG
                     DIEAWSKSAASQLDSQLRERRKNFVRRLEAIDRIEQASNGLDQRIAEIDQRSVEIDQV
                     ELKLVELTDYLLLMTAPERFGVAADDASA"
     gene            complement(6212..7027)
                     /gene="mutM"
                     /locus_tag="PLB25_08155"
     CDS             complement(6212..7027)
                     /gene="mutM"
                     /locus_tag="PLB25_08155"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HOE41584.1"
                     /translation="MPELPEVEVTRRSFVRAIEGAQVLAAQLGKSLRWPLGCEPSLLV
                     GRFVRTVHRRGKYLQIELDHGWLLLHLGMSGSLCFAADLPQAGIHDHFDLVTSRGTLR
                     LNDPRRFGAVIYLDSLADPMAHKLLGHLGLEPLSDSFGLAEFHAGLKQRKAAIKQVLL
                     AGDLVVGVGNIYASEALFAAAIRPTASSASLGRVRVARLHAAICMVLARALEQGGSTL
                     RNFSSTDGQTGHFQLDAMVYGRADLPCKVCAKPIRMIRQGQRSTFYCPSCQKR"
     gene            7169..>8174
                     /locus_tag="PLB25_08160"
     CDS             7169..>8174
                     /locus_tag="PLB25_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41585.1"
                     /translation="MDSLLFYQLLVGELNARSEEPDAAFALLLDAARKTADPAVYRRA
                     IQIAIQARAGESALKAAKAWSQAIPASAQAHRYTWQILLGLNRTAETLEPLKREIALT
                     PAGELRDLLWALPGNYLRASDRRLAASTVQGALAGVLNDKAVGATAWAVVGRFWLAAS
                     DKPAALSAANKSFAIDNKSEHAALLALSLMSPDLPPAENLVKQHLPHARPEFRMAYIK
                     ALLDVHRTDEATAELEIIKTRSPGYADAWLIDGALALQMKQLPLAEQQLQRYLDLVEG
                     QPLDQQAPEIRRGRAQALMSMAQIAQLRKQPEKADAWLQRVDNPDDVLRAQIRRASIM
                     A"
BASE COUNT         1721 a         2460 c         2366 g         1627 t
ORIGIN      
        1 cattaccgac attccctatg acagctttac cgccagtctg ggcaagatgc ttcagggcga
       61 atatgtggag gaccatcgca gcctgatgaa ggcctgtgtg atacgcggtg gtcactgcgt
      121 tttcaaggcc atggccatga atgatgtcgt ggtgaatcgg ggggctaccg ctggcatggt
      181 ggaattgcgg gttgaagtcg atggccattt tgtcgccaac cagcgcgccg atggcctgat
      241 cattgccacg ccaaccggtt ccaccgccta cgcgctgtcg tcgggtggcc cgttgctgca
      301 cccatccatc cccggctggg taatggtgcc gattgccccg cataccttgt caaatcggcc
      361 catcgtcctt gccaacacct cgcacatagc gatcgagatc gcctccgggc gtgatgccag
      421 cgccagtttc gacatgcaat ccctggcatc cttgatgcac ggtgaccgaa ttgaagtcac
      481 ccgctcggct caccatgtgc ggtttttgca ccccaggggc tggtcttact ttgacacgct
      541 gcgcgaaaag ctgcattgga acgagggggt agcgacgacg tgagccttaa acgaattgtg
      601 ctgcgtgact ttgtcatcgt cagcgaactt gagcttgagc tccacagcgg ctttaccgtg
      661 ctgaccggtg agaccggtgc tggcaaatcc attctgatcg atgccctgca gttggtcact
      721 ggtggccggg ctgacctgac catgattcgt gaaggtgcca gccgcaccga tatcagcgca
      781 gagtttgacg ccacgccggg cattgcatgc tggctgcaag aggctggctt tgaaaccgac
      841 gcagccagcg gtctgctgct gcgccgtacc gtggatgtgc agggcaagag ccgcgcctgg
      901 atcaacggta gcccggccac tgccacacag cttcgggcgg tgggagagca gcttctggac
      961 attcatgggc aacatgcctg gcaaagcctg acgcgcgcag atgcggtcag gggtttgctc
     1021 gatgcttacg ccaaggtttc caccacgcag gtggcggtcc tgtggcagca atggcacacg
     1081 gcgcaagagc ggctgacgca ggcgcgcgca tcgcaagagt cgctgctgca agagcgagaa
     1141 cgcctgacct ggcagattgg cgagcttgaa aaactggccc cactgccgca cgagtggcca
     1201 accttgaatg ccgagcacag ccgactgtcc catgcgcaag ccctgttgga cgcggcgcaa
     1261 atggcactgg cagtgctcag cgaggacgat gacaatgcca ttgggcgtat cgggacagct
     1321 catgatttgc tacaaaaaca agagcttata gagcccgtct ttaaagggct gaccgaggtt
     1381 ttgagttcga gtctggcgca gctcgaagat accgtgcaca cgctgcgcgc gtacctgcgt
     1441 aaaaccgagc ttgaccctga tcgactgagc acactcgacg acagattggg gttgtgggtg
     1501 tcgctggcgc gacgctacaa acgtgtcccg gaggaactgc ccgagttgtt gaaccattgg
     1561 aagcaggcgc tgctggtgct cgatgctgcc gccgatctgc agcaacttga acaagcgctg
     1621 gcttccgcct ggcaggccta tttgcgcgaa gcgcaggcgc tgacacaggt gcgcaacggg
     1681 gctgctgtcc aactggccaa aaccatcagc aacgcaatgc agggcttggg catgcagggg
     1741 gggcagtttg aggtgcagct tcagccagca gcgcagccac tgcaaagtgg gctggaagac
     1801 gtgagttttc tggtggcggg gcacgccggc agcacgccgc gtgcggttaa caaggtcgcg
     1861 tctggcggcg aactgtcgcg catcgctttg gccattgctg tcaccaccag ccagctcggc
     1921 accgcccaga cgctgatttt tgacgaggtc gatgccggcg tgggtggtgc cgttgcagaa
     1981 actgtgggca agctgatgca ccaactcggc gttgatcggc aagtgctggc ggtaacccac
     2041 ctgccccagg tggcggcttg tgctgatcag cacctggtgg tgagcaagaa gctccagggt
     2101 ggctgcacca tcagttcggt ggccagcgtg aaggccgaag agcgggtcac agaactggcc
     2161 cgcatgctgg gcggtgagcg cattttggcg accaccctgg cgcacgcccg cgacctgctg
     2221 ttgacccagg agccctgata tggaggcatc accccaagga ttgcaaattg tcctgatcac
     2281 cggcatgtcc ggttcaggca agtcaatagc gctgcatgcg ctggaggatc tgggcttcta
     2341 ttgtgttgac aatttgccac ccgagttgtt tttgccattg gttgagttgg agcatgcgaa
     2401 ccacgccagc cggctggcta ttgcgatgga tgtgcgcagc gcaggttcgc tgccgctgct
     2461 accggcacag ttcaggcaac tcaaggcggc aggttttcag tttgactcga tttttctgga
     2521 tgccagcacc gacaccctga tcaggcgctt ttccgaaacc cgcaggcgtc accccttgtc
     2581 ccagggaagc cacagcggtc atgtgctgga ccagcggcac gcgctggccg aagcgattga
     2641 actcgagcgt gaacttctga gcgacctgcg cagtcaggcc catgtgattg attcgagcat
     2701 gattcgcccg acgcaattgc aaagctatgt cacggcattc atcgaagccc ccggcgagca
     2761 gttgaccctg gtgtttgagt ctttcgcctt caaacgcggc attcccagcg acgctgacta
     2821 tgtgtttgac gtccgcatgc tgcccaaccc gcactacgag cctgcgttgc gtgcattgac
     2881 cggcatggac gcgccagtga ttgacttcct gaggcaggag caagatgtgc agcgcatgca
     2941 agacgatgtc cgcgcctttc tgacccattg gcttgaagcg ctggcgcgca atcatcgcag
     3001 ctatgtcact gtggcaattg gctgcaccgg cggacagcac cgctcggtct atttggtgga
     3061 acaactggcc gcctcgttcg cggcgcgctg ggtcaccctc aagcgacacc gtgaaatgga
     3121 tgcccgttag cggccaggtt caccgttcaa cagtttcctt atgggcttcg gcagccccag
     3181 ttgtggccat tgatgcgcgt cgatccatgc gccacgggcc actggctggc aggtgcgcgg
     3241 caaaagaacc cgtaccggat gcaggtacag gtctttgtgc gtcagcacat gtttgaaggc
     3301 tgattcgtca cgcaattggg ccgacaagat gcctggcacg gccaacagca attcagcacg
     3361 gtcgttaaac agcgcaaagc aatacaagcc agcccagacc cctggtgttg ggcgctggtc
     3421 aagccacagt gcaccgtcat ccgcctgcgc ccacaacatc cagatcgact gggcgcttcg
     3481 cttgagtttg cgtgtgttta ccgggaactt cccgggattc gcccgcgccc tggcctggca
     3541 atgggcctga agcggacaaa cgtcacagtc gggctttttg gcggtacaaa ccgttgcccc
     3601 caggtccatg atgccctggg tgtaacgcgg catgtcagtt gcgagtgcat cctgcggcag
     3661 caattcattg gccagttgcc atagtcgtcg cagattggcc gcttgcgcca agtctgcgtc
     3721 aaaacccaga aagcgcgtca gcacccgttt gacgttgcca tcaagaatcg ccacccgctc
     3781 agcaaaacac agggatgcaa tcgccgccgc cgttgacggg ccaatgcccg gcaaggtttg
     3841 aagttggctg gcacttcgcg gaaaagcccc gccgtgcaaa cgcatcacat cctgtgcgca
     3901 gcggtgcaaa ttgcgcgccc ggctgtagta gcccaaacct gcccacaacg ccaataccga
     3961 atccaatgag gcctcagcga gcgccctgac atcaggaaac tgcgccagaa accgttgaaa
     4021 atattcgccc accgtgacca cctgggtctg ctgcagcatg atctcagaca gccagacgcg
     4081 atacggatct tgcgtgtttt gccagggcag atcctgtcgg ccgtgttgcc gctgccaccg
     4141 cagaagggcg ccagacagag aaaaaccacc ggaccagggc tcaggcagag gcatcgtccg
     4201 cggccacgcc aaaacgctct ggcgcggtca ttagcagcag gtaatcagtc aattcaacca
     4261 gtttgagttc aacttgatca atctcaacgc tgcgctgatc aatttctgca atgcgctggt
     4321 caaggccatt ggaagcctgc tcaatgcggt caatggcctc cagacggcgg acaaaattct
     4381 tgcggcgctc gcgcagttgc gagtcgagtt gcgacgcagc cgatttgctc caggcttcaa
     4441 tgtcacccag tgcggtttca taaactcccc gcaagcgcgt tgacaaggcc cgaaccacct
     4501 gctgtgcaaa ctcgggtagc gccagcctga aggcattgcc gatgccaagg tactgcaggt
     4561 gactgcgttc aatcaaatcc agttcagcca aataggtgtc aacctggggc tcttcgggca
     4621 cctgcaacga aaaaccatat tcggtgttca actgcaaaaa cacggccacc agcatggtgt
     4681 gaatttccag cccgttggca tgcaccagcc gcaggttggc ccgcagaccg tcaaaggttt
     4741 cgccatatga ccgttttatg cctagcttga aacctttttg attcagcgat gttgtcaact
     4801 gcgccatttc gcgcttcagg ttgaccggac ccagcagatt gaagatctca cgcaacaact
     4861 tgagatgaac tgatcgcacg gcatggatct tgacacccgc ggcgtcaaac tcggcctgtt
     4921 cctgggcaat gcgggagcgc atatttttga tcaccggccg atttttgccg cgcaagcctt
     4981 tgagctcaat caactgttcg gtcaggtcac gccgacggat atgcaccacc cgtccggttt
     5041 cactgcgtag cgcggcaatg ccgcgcgcca cggctgcacc cagtattttt tgtcgccgac
     5101 ccataatgcc ccggctcaag gcatcctcca gatcgcccag gcaactccgg gtcagcaggg
     5161 cggcatcgtt ggtgaccttg ccgaccaagc ctttttgcgc agatatcgcc accacttgct
     5221 gctcggaaat accgagaatc cgggccgaat cgcgcctttg ccggtcaatt tgggcttgca
     5281 cctcttgcgg gctgtccagc gaatcccaaa gggtgtcgat tttgttaagc accaccaacc
     5341 gggttgtcac atcctggtca tcatctacca aatgttcacg ccaaatggac agatcggatt
     5401 gggtcacgcc ggtatctgcc cccaggatga acaccactgc ctgtgcctga ggaatcaggc
     5461 tgacagtgag ttcaggctct gccccgatcg cgttcagccc cggtgtgtcg agaatgatca
     5521 ggccttgctt gagcagcggg tggccgatat tgatcagcgc atgtcgccat ctgggcacat
     5581 caacgagtgc gccgccatcc ggcgcggcat tgtgttgtgc cgtcaggtgt tgccaaaagc
     5641 ccagcgcccg cgcttcgtcc agggtgaccc ggcgggtttc agcgaccttg gcaaatgctt
     5701 cagcgagttg tttggggttg ttgacatcaa gctcggtgcg ctcccatttt tcgggcacca
     5761 tacgccactc catcagcgac tgcggttgca aacgggtctc aatgggtaac aagcgcagac
     5821 aaggtggaac attcgggtcg taacccatct cggtcgggca catggtggtc cgcccggcgc
     5881 tcgctggcat gatgcgctgc ccataatcgg caaaaaacac tgcgttgata agctcggact
     5941 tgccccgtga aaattcggca acaaaggcca ccatgacctt gtcaacccga atctgggctt
     6001 caagccgatg cagccggtct tccactgaag catccagcat gtcctggtcc ttcaaccact
     6061 gcgccagcaa tttcagccgc aacgcaaact cgcgccgcca tacgccgtgc tggtcaaatt
     6121 tctcattgaa tgaagttccc aaggtatctc caatacatgc ctagcgaatc aatatagcac
     6181 tcttgcgccg gttaaccctc gaaaatgcaa atcagcgctt ctggcagctt gggcaataaa
     6241 aagtggaacg ctgaccttgc cgaatcatcc tgatgggttt ggcgcagact ttgcatggta
     6301 agtcggcccg cccgtaaacc attgcatcca actgaaaatg accagtctga ccgtcggtac
     6361 tggagaagtt gcgcaaggta ctgcctccct gttccagcgc ccgcgccagc accatgcaaa
     6421 tcgccgcatg caggcgcgca actcgcaccc ggcccagact ggcagaactg gccgtcgggc
     6481 gaattgcagc cgcaaacagg gcttctgagg cataaatatt gcccacgccc accaccagat
     6541 cgccggccag caagacctgt ttgatggccg cttttcgctg tttcagaccc gcatgaaact
     6601 cagccagccc gaatgaatcc gacagcggct cgagcccaag atgcccgagc aacttgtgcg
     6661 ccatcgggtc agccaggcta tcgagataga tcacggcacc aaagcgacgc ggatcattca
     6721 ggcgcagcgt gccacgtgaa gtcaccagat caaaatggtc gtggatgccc gcctgcggca
     6781 ggtcagcagc aaagcacagg ctacctgaca tgcccaaatg caacagcagc cagccgtgat
     6841 caagttcaat ctgcaggtac ttgccgcgcc tgtgaacggt gcggacaaaa cgccccacca
     6901 gcagacttgg ctcgcagccc aatggccagc gtagcgattt gcccagttgc gcggcgagca
     6961 cctgcgcacc ttcaatggcg cgaacaaaac tgcgccgggt cacttctact tcgggtaatt
     7021 caggcatgcg aaaaatatat tgatgagcgt ggattattat gggcggatgc gcttcaaacc
     7081 tgttttagta cctttgctgc tgagcctcgc ctttggcctg catgcccaaa gtcccgccaa
     7141 atccgagcct ggttttaaaa gttcggccat ggacagcctg cttttttacc aattgctggt
     7201 aggcgaactc aatgcccggt ccgaagagcc agacgctgcg ttcgcgctgt tattagacgc
     7261 tgcccgaaag acggctgacc cggccgttta cagaagagca atccagattg caattcaggc
     7321 gcgggccggc gagtcggccc tgaaggccgc caaagcctgg agccaggcga ttccggcatc
     7381 cgcgcaagcc catcgctata cctggcaaat tttgctcgga ctcaatcgca cggcagaaac
     7441 gcttgagccg ctcaagcgcg aaatcgcact aacccccgcc ggggagctgc gcgacttgct
     7501 atgggcactg cccggcaact acctgcgcgc cagtgatcgg cggcttgctg ccagcacggt
     7561 gcaaggtgcg ctggcgggtg tcctcaacga caaggcggtt ggtgccacgg cgtgggctgt
     7621 tgtcggccgt ttttggctgg ctgccagcga caaacctgcg gccctgagtg cagccaacaa
     7681 atcattcgcg atcgacaaca agtccgagca cgccgccctg ctggcgctat cgctgatgag
     7741 ccccgatttg ccaccggcag aaaacctggt caaacagcac ctgccccacg cgcggccaga
     7801 gtttcgcatg gcctacatca aggccctgct ggatgtccat cgcacggacg aagccacagc
     7861 cgagctggag atcatcaaaa cacgctcgcc tggttacgct gatgcgtggc tgatcgacgg
     7921 tgcgctggcg cttcaaatga aacaactgcc gttggctgaa cagcaattgc agcgctatct
     7981 tgatctggtg gaaggccagc cgcttgatca acaagcgcca gaaatccgac gcggaagagc
     8041 gcaggcgctg atgtcgatgg cccagatcgc gcaattgcgc aagcagcctg aaaaggcgga
     8101 tgcctggctg cagcgggttg ataacccgga cgacgtgctg cgtgcccaga ttcgccgcgc
     8161 cagcatcatg gcgc
//
LOCUS       DAOSLL010000144         8122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8244_length_8122_cov_4.442296, whole genome shotgun sequence.
ACCESSION   DAOSLL010000144 DAOSLL010000000
VERSION     DAOSLL010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8122
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8244_length_8122_cov_4.442296"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="PLB25_08165"
     CDS             complement(<1..1220)
                     /locus_tag="PLB25_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HOE41586.1"
                     /translation="MQSRVLEWPRWVKRLVVVALDIILALFATWVSYALRLDELHWPT
                     GAQWWVYLLAPTLAIPVFIRFGLYRAIFRYTGQAALVATGLAVLVYGSVLLGILVWMH
                     WPMVPRSLGILQPLVFLFLVGASRASARFWLADLGLSREQFEGRLLIYGAGTAGVQTA
                     SALRLSQQYLLLGFIDDDPAKVGRSINGTPVYSPTEVAQVIARQGATEMLLAIPSASR
                     DRRNAILESLRHLPIHIRTLPGLSDLASGRVTIQDFRELDVEDLLGRDPVPPDASLLA
                     KDLAAQVVLVTGAGGSIGGELARQIALQRPSQLLLFDHNEFGLYSIHRELQEICARTG
                     LSVELVPLLGSVANPERLASICRAYRPALVYHAAAYKHVPMVEDNPGEGILNNVFGTL
                     NMAQAAQDSGVARFV"
     gene            complement(1241..1555)
                     /locus_tag="PLB25_08170"
     CDS             complement(1241..1555)
                     /locus_tag="PLB25_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HOE41587.1"
                     /translation="MTNLIEIQNQIAVLQKQAEEIKAQEFAKTVQEILAKMEAFGITV
                     SDLDGSRDRPRKLSTKSANPAPAKFRGPNGETWSGRGLMPRWLAALVAQGQTKESFAI
                     QA"
     gene            complement(1615..2175)
                     /locus_tag="PLB25_08175"
     CDS             complement(1615..2175)
                     /locus_tag="PLB25_08175"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HOE41588.1"
                     /translation="MKRSCDLLLALLAMLLLALPILFVGLLVRLTSTGPVLYWSDRVG
                     RNNQLFRMPKFRSMQVGTPAVATHLLGNPDAFLTPVGSFLRKSSLDELPQIWSIAKGD
                     MSFVGPRPALFNQHDLIGLRTVAGVHLLRPGLTGWAQINGRDELPIPEKVALDIEYLH
                     RRSFFFDTKIILLTAFRVLKRDNVAH"
     gene            complement(2179..3117)
                     /locus_tag="PLB25_08180"
     CDS             complement(2179..3117)
                     /locus_tag="PLB25_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE41589.1"
                     /translation="MKILVTGANGLVGRALCALLDDRGIPVRAAVRSASPISPGLDCV
                     GIGSIDGQTCWSCALAGTNTVVHLAARVHVMNEAAADPLAEFRRVNVLGTLNLARQAA
                     AAGLQRFVFVSSVKVNGELTQAGQAFSESDSPSPEDHYGISKWEAEQGLLQIARETGL
                     QVVIIRPPLVYGPGVKANFAALMCAVQRGWPLPLGAVNNQRSLVALDNLVDFIALCVV
                     HPDAVNQVFLISDGKDVSIAGLIRGIALAAGVPARLWPVPLWVLQAAGHLTRRSEVVQ
                     RLCSNLQVDISKAQKVLGWKPVISLSEGLHRAFKGR"
     gene            complement(3114..3893)
                     /locus_tag="PLB25_08185"
     CDS             complement(3114..3893)
                     /locus_tag="PLB25_08185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE41590.1"
                     /translation="MEKVVQSDSVAISVSVVSHGQMHLVVHLLQDLQTHCSGQNIELI
                     LTLNVDENLQLERTHFSFPIQIIVNSAPKGFGANHNQAFRAARGSYFCVANPDIRLNA
                     CPFTGLMACLAGADVGVTAPLVLDPSGRIEDGARRFPTPGKIMVKAFSGPQIPDYSFQ
                     AGSQHVDWVGGMFLLFPGSVFRDMNGFDERYFLYYEDVDICARLSLANFHIVLFSGTY
                     VVHHAQRSSHRRLKYLVWHLCSMVRFFMSGPYQRLKRLGRL"
     gene            complement(3881..4702)
                     /locus_tag="PLB25_08190"
     CDS             complement(3881..4702)
                     /locus_tag="PLB25_08190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE41591.1"
                     /translation="MISGPVLVPVSVIIPCFRCARTIERALASVAAQTVLPTEVILID
                     DASGDETRATLRELEGRYQPGWVKLVLLDHNVGAGSARNAGWAIASQPLIAFLDADDA
                     WHFRKLEIQYDYMAANPEVDLSGHGFRLLKQGVLPYWMVQKGTATPIGKWRMMLSNRF
                     VTPSVMLRRDVAYRFVERQRYMEDHMLWLRVVCAGARVVKLPAELAAIYKDPFGVTGL
                     SSQVWLMERSVLGNYHRLYQDQSINLIQFAALIVYSTLKYLRRLVIYTAYLRWKK"
     gene            complement(4699..5550)
                     /locus_tag="PLB25_08195"
     CDS             complement(4699..5550)
                     /locus_tag="PLB25_08195"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE41592.1"
                     /translation="MNFNDGLPLISVVIAVYNGSSTLQQCIDSVNQQTYQKLELIVMD
                     GGSTDGTVDLLIANAQKLTYWATEPDTGIYNAWNKALKKAKGDWICFLGADDYFWDAQ
                     VLAHMAEKLAVVSPDINVAYGQIMLLAKGDEPMYPLGQPWEIVKARFRREMCLPHPAV
                     MHRSGLFRRLGAFDESFRIAGDYELLLRELKDHAAIFIPDLIVTAMRQGGVSSSPNST
                     LTGLHEVRRAQKKAGLVWPSPNWFLALVRTYVRLILWGLAGEALTRRLLDLGRRVLGL
                     PAYWTRT"
     gene            complement(5547..6500)
                     /locus_tag="PLB25_08200"
     CDS             complement(5547..6500)
                     /locus_tag="PLB25_08200"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOE41593.1"
                     /translation="MRALICGIGGQDGAFLARLLLEKGYEVWGTSRDAQIVEFRNLKM
                     LQILAQIKLCSMAPTDFRSVLSVLKRSAPDEVYFLAGQSSVGLSFEQPVETLESITVG
                     TLNMLEAIRFLGGQCKFYHASSSECFGHLDQTSANEQTPFHPRSPYAVAKASAHWMVA
                     NYREAYGLYACSGILFNHESPLRPARFVTRKIVDGALRIANGANEKLVLGRLDIFRDW
                     GWAAEYVDAMWRMLQLEEPQDFVIATGVCVSLETFVSSVFTRVGLQWRDHVCSDPLLF
                     RPSDIGWSQGDASLAKQRLGWEAQTKMPELANMLVEAAAVR"
     gene            complement(6497..7600)
                     /locus_tag="PLB25_08205"
     CDS             complement(6497..7600)
                     /locus_tag="PLB25_08205"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HOE41594.1"
                     /translation="MKVIYDCQIFSAQEFGGISRYVCSLASQMSGFSGVEARIVAPIH
                     INGHLKSIDSGIAVGCHVRRLPKTSRMIKAVSAALCGPIASALRPDLVHETYYAQQSP
                     CRSKVPHVLTVYDMIEERFPASFPDGYPVARLKRAAVNRADHVFCISENTRRDLMELY
                     RLGPDRISVTYLGYDQLPVSGLTASDLVGESPYILHVAGRYGYKNFEGLLRAYAASGW
                     LKSHFRIVCFGAAPFTLLERSLFAQLGLSEEKVTHCGGSDERLAALYKGAAVLVYPSK
                     YEGFGIPPLEAMSQDCPVICSSSSSIPEVVGNAGEYFDADEPDSIRAAMEKVLQSPER
                     RDELVLLGSDRCKLFTWTRCAQETLDVYRRLAA"
     gene            complement(7613..>8122)
                     /locus_tag="PLB25_08210"
     CDS             complement(7613..>8122)
                     /locus_tag="PLB25_08210"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOE41595.1"
                     /translation="RLLRDAGFDFYWSDKYCDNLFVPGFEYAPERGSCRVVTAMEVME
                     HLADPITFVEETLALCNAQTLLFSTELYQGAPPSPDQWWYYTFETGQHIGFFQRRTLE
                     VLGAKLGLRFCTANGLHVLSRQPVDESLLGWATGRWASLFSMWWVRRQLGSKTLSDHQ
                     MMLKNISDQ"
BASE COUNT         2045 a         2424 c         1979 g         1674 t
ORIGIN      
        1 acaaagcgcg ctaccccgct gtcttgcgct gcctgagcca tgttgagggt gccaaacaca
       61 ttgttcagaa tgccctcgcc cgggttgtcc tcaaccatcg gcacgtgttt gtaggccgcc
      121 gcatgataga cgagggccgg gcggtacgct cgacaaatgc tggccaggcg ctcggggttg
      181 gcgacgctgc ccagcagggg caccaattcg actgacagcc cggtcctggc gcaaatctct
      241 tgcagttccc ggtgaatgct gtataagcca aactcattgt ggtcgaacaa caacaattga
      301 cttggccgtt gcagcgcaat ctggcgcgcc agttcaccac caatgctgcc gccggcacct
      361 gtcacaagta ccacctgagc ggccagatcc ttggccagca gcgatgcatc tggcggcacc
      421 ggatcacgcc ccagcaaatc ttctacatcg agttcccgaa aatcctggat cgtgacgcga
      481 ccactggcca agtcggacag cccaggtaaa gtgcggatgt gaatcggtaa atgccgcaga
      541 ctctccagaa tcgcattgcg ccggtcacgc gacgcactgg gaatcgccag caacatctcg
      601 gttgcgcctt gccgggcgat cacctgcgct acttcggtgg gtgagtaaac cggtgtccca
      661 ttgatgctgc gtcccacttt ggcagggtca tcatcgataa agcccagcag cagatattgc
      721 tgcgacaagc gcaaggcaga ggcggtttgc acgcccgccg tgccggctcc gtagatcaac
      781 agccgtcctt caaattgttc gcgcgaaagc cccaggtcag ccagccagaa acgcgcgctt
      841 gcccgactag cgccaaccag aaacaaaaat accaatggtt gcaggatacc caggctgcgc
      901 ggcaccatcg gccaatgcat ccataccagg atacccagca ggacgctgcc gtagaccagc
      961 actgccagac cagtggccac cagggccgct tgaccggtat aacgaaaaat ggcccgatac
     1021 aggccaaaac gaatgaatac ggggattgcc agcgtcggtg ccagcagata aacccaccat
     1081 tgggcaccgg tgggccagtg cagttcgtcg agcctcaggg cataggatac ccaggtagca
     1141 aagagagcca gaatgatgtc aagagcaaca accaccagcc gcttgaccca acgcggccat
     1201 tccagaaccc tgctttgcat tgaaccgccc tgatgtcgcc ttatgcctga atcgcaaatg
     1261 attctttggt ttgaccttgc gctaccagcg cagccagcca gcgcggcatc aaaccgcgtc
     1321 cgctccaagt ttcgccatta gggccacgga acttggcggg cgcaggattg gcagacttgg
     1381 tgctaagctt gcgcgggcga tcacgagaac cgtcaagatc agacacagta atgccgaatg
     1441 cttccatctt ggccagaatt tcctgaaccg ttttggcgaa ctcctgtgcc ttgatctcct
     1501 cagcctgctt ctgtaaaacg gctatctggt tttgaatttc aatcaaattt gtcatgttta
     1561 gctcctttaa gtaaaaattg ttaaagttcc tgatggtctg gaaggcatat tgttttaatg
     1621 agccacatta tcccttttta aaaccctgaa tgcggtcaaa agaataatct tggtgtcaaa
     1681 aaaaaatgat cgtcggtgca gatattcaat atccagggca actttttctg gtatcggcag
     1741 ttcatcccga ccgttgattt gcgcccaccc cgtcaagccg ggccgcaaca gatgaacccc
     1801 ggcgactgtt cgcaggccaa tcagatcatg ctggttaaac agcgccggcc ttggaccaac
     1861 aaaactcata tccccttttg caatgctcca tatttggggt aattcgtcga gactactctt
     1921 gcgcagaaaa cttccaactg gtgtcaggaa cgcatctgga ttaccaagca aatgggttgc
     1981 cacagcgggt gttcccacct gcatactgcg gaacttgggc atcctgaata actggttatt
     2041 gcgtcccacc cgatctgacc aatacaacac cggtcctgtc gatgtaagcc gcacgagtaa
     2101 accgacaaac aggataggca acgccagcag caacatagcc aacagcgcca gcaacaaatc
     2161 acaacttctt ttcatggttt atcggccttt gaacgccctg tgcaaaccct ctgacagact
     2221 gatgacaggc ttccaaccca gcactttttg cgccttgctg atatccacct gcaaattgct
     2281 gcacaggcgc tgcaccacct cacttcttcg cgttaagtgc ccggcagctt gcaacaccca
     2341 aagcgggact ggccagagcc gggcgggcac accggctgcc agtgcgatcc cacgaatcaa
     2401 cccggcaata gacacatctt tgccgtcact gatcagaaaa acctgattga cggcatccgg
     2461 gtggacgaca cacagcgcga tgaaatccac cagattgtca agggctacca ggctgcgttg
     2521 gttgttcaca gcccccagag gcaagggcca gccacgctgc acagcgcaca taagggcggc
     2581 aaaattggct ttcacgccag gcccgtaaac cagcggtggc cggatgatga caacttgcag
     2641 cccggtttca cgggcgattt gcagcagacc ctgctctgcc tcccacttgc ttattccgta
     2701 atgatcttct gggctaggtg aatcagactc cgaaaaagcc tgaccggctt gcgtcaactc
     2761 cccattgacc ttgaccgaac tgacaaacac aaatcgttgc agtcccgctg ccgctgcctg
     2821 acgcgccaga ttcaaggttc ccagtacatt cacccggcga aactcagcca gcgggtctgc
     2881 tgctgcctcg ttcatgacgt gcacccgggc tgccaaatgc accacagtgt tggtaccggc
     2941 cagcgcgcac gaccagcagg tttgcccgtc aatactgccg attccgacgc agtcaagccc
     3001 aggcgaaatc ggtgaagcgg accgcacagc agcgcgaact ggtattccac ggtcgtcaag
     3061 cagcgcacac aaagcgcggc ccaccagtcc attggcaccg gtaaccagga ttttcataag
     3121 cgacccagac gcttcagccg ttggtatggg ccagacatga agaaacgcac catgctgcac
     3181 agatgccaga ccaggtattt caagcgccgg tgactactgc gctgcgcatg gtgaaccaca
     3241 taggttccag aaaaaagtac gatgtgaaaa tttgccagac taagtctggc acaaatatca
     3301 acgtcttcgt aataaagaaa ataccgttca tcaaatccat tcatatcccg aaaaaccgac
     3361 ccaggaaaca gcaaaaacat gccaccaacc caatcgacat gctgtgatcc ggcttgaaag
     3421 ctgtagtcag gtatttgagg tccggaaaat gccttaacca ttattttgcc gggcgttgga
     3481 aatcgacgcg caccgtcttc aatccggcca gaaggatcca ataccaacgg tgcagtaaca
     3541 cccacatcag cgccagccag acacgccatc agtccggtaa acggacaggc attcaaccgg
     3601 atatccgggt ttgcaacaca aaaataactt cctcttgcgg cacgaaaagc ctgattgtga
     3661 ttggcgccaa accccttggg cgcagaattg acaatgatct ggatcggaaa cgaaaaatgg
     3721 gtcctttcca attgcaggtt ttcatcgacg ttgagcgtca gtatcagttc gatattttgc
     3781 ccgctacagt gcgtttgcag atcctgcaac aaatgcacaa ccaaatgcat ttgtccatgg
     3841 ctgaccaccg agacggaaat agccacagaa tcagattgga ctactttttc catcgcagat
     3901 atgccgtgta aatcaccagt cgccgcagat atttcagcgt tgagtaaaca atcaacgccg
     3961 caaactgaat caggttgatg ctctggtcct ggtaaagccg atggtagttt cccaggacgc
     4021 tacgctccat cagccaaacc tgcgagctca gccctgtaac gccgaatggg tccttgtaaa
     4081 tcgccgccaa ttctgcaggc agtttcacca ccctggcacc ggcacacacc acccgaagcc
     4141 atagcatatg gtcctccatg tagcgctgtc gctcaacaaa ccggtacgcc acatcacgcc
     4201 tgagcatgac agatggcgtt acaaaccggt tggagagcat catgcgccac ttgccaatgg
     4261 gcgtagccgt gcctttttgc accatccaat aaggcaaaac gccttgcttg agcagccgaa
     4321 acccatgccc ggacaaatcg acttccggat ttgctgccat gtagtcgtac tggatttcca
     4381 atttgcgaaa atgccaagca tcatcggcat caagaaacgc gatcagtggc tgactggcaa
     4441 tagcccaacc ggcattgcgt gcgctgccag cccccacatt gtgatccagc aaaaccagct
     4501 tgacccagcc cggctgatac cgtccttcaa gctcgcgcag cgtcgcccga gtctcgtcac
     4561 cactggcatc atcgatcagt atcacttctg tcggcagcac cgtttgtgcc gccactgaag
     4621 cgagcgcccg ctcaatcgtg cgggcgcagc gaaagcaggg aatgatgacg ctgacgggaa
     4681 ccaaaaccgg tccgctgatc atgttctggt ccaataggcg ggcaaaccca gcaccctgcg
     4741 gcctaggtca agcagtcttc tggtcaaggc ctccccggca agcccccaga gaatcaagcg
     4801 cacataggtt cgcactagcg ccagaaacca attcgggcta ggccaaacca ggcccgcttt
     4861 tttctgtgcg cgccgtacct catgcaagcc tgtcagcgta ctgttggggc tgctgcttac
     4921 cccaccctgc cgcattgccg tgacgatcaa gtcaggaatg aaaattgccg catggtcttt
     4981 cagttcacgc agtaacaatt cgtagtctcc ggcaatgcga aacgactcgt caaatgcgcc
     5041 cagccgccga aacaagccac ttcgatgcat gaccgccgga tgaggcaggc acatttcccg
     5101 ccggaaacgg gctttcacga tttcccatgg ttggcccaac ggatacattg gctcgtcacc
     5161 cttggccaac agcatgatct gcccgtaagc cacgttgatg tcaggcgaca caacagccag
     5221 cttttctgcc atgtgggcga gcacctgggc atcccaaaaa taatcatctg cacccaagaa
     5281 acaaatccag tcgccctttg cctttttcag cgccttgttc caggcgttat agattcctgt
     5341 gtcgggctca gtcgcccaat aggtcagctt ctgcgcgttg gcaatgagca agtccactgt
     5401 gccatcggtt gagccaccat ccatcacgat caactcaagc ttctggtaag tctgttgatt
     5461 cacgctgtcg atgcattgtt gcagggtgga actgccgttg tacacggcaa tcacgacact
     5521 gatcaatggc agtccgtcgt tgaagttcat cgcacggctg ccgcctcaac cagcatgttg
     5581 gccaattccg gcatttttgt ctgcgcctcc caacccagcc gctgttttgc cagacttgca
     5641 tccccttgac tccagccgat atcagatgga cgaaacagca aagggtcgct gcacacgtga
     5701 tcacgccatt gcagcccgac acgtgtaaaa acgctgctga caaacgtttc cagcgataca
     5761 caaacaccgg ttgcgatgac aaaatcttgc ggttcttcga gttgcaacat ccgccacatg
     5821 gcatcaacat actctgccgc ccagccccag tcgcgaaaaa tgtccaggcg tcccaaaacc
     5881 agcttttcat ttgccccgtt ggcaatgcgc aatgcaccgt caacaatttt tcgtgttaca
     5941 aagcgagcgg gtcgaagcgg cgactcatgg ttgaacaaaa tcccgctgca ggcatacaga
     6001 ccataggctt cacgataatt ggcgaccatc cagtgggccg atgccttggc aacggcgtaa
     6061 gggctgcgtg gatgaaaggg cgtctgttca tttgctgagg tttgatcaag gtgaccgaag
     6121 cactcgcttg aactggcatg gtaaaacttg cattgcccac ccaaaaatcg gatcgcctcc
     6181 agcatgttca gagtgcctac cgtgatgctc tccagcgttt cgactggctg ctcaaatgac
     6241 agacccacag aactttgacc agccaaaaaa tacacctcgt ctggtgcgct tcgtttcaac
     6301 acggacaaca cgcttcgaaa atcggtcggt gccatcgagc aaagcttgat ctgcgcaagt
     6361 atctgcagca ttttcagatt ccggaactcg acgatttgtg catccctgga tgtcccccac
     6421 acttcatatc ctttttcaag caacaagcgg gccagaaaag caccgtcctg accaccaatt
     6481 ccacaaatca aagctctcac gcagcaagcc tccggtaaac atcaagggtt tcctgcgcgc
     6541 agcgcgtcca ggtgaacagc ttgcagcgat cactcccgag caacaccaat tcatccctgc
     6601 gctcaggcga ttgcaatacc ttttccatcg cggccctgat ggagtcgggc tcatccgcgt
     6661 caaagtactc gccagcattg ccgacaactt ccggaattga actgctgctg ctgcaaatga
     6721 ccgggcagtc ctgtgacatg gcctcaagcg gaggaatacc aaaaccttca tatttggagg
     6781 gatacaccaa cactgccgca cccttgtaca aggcggccag acgctcatca ctgcctccac
     6841 aatgcgtcac tttttcttcg gacagaccca actgcgcaaa gagtgagcgt tccaacaagg
     6901 taaaaggggc cgcaccaaaa cagacaattc ggaaatgact tttaagccaa ccagacgcgg
     6961 cataggcacg aagcagacct tcaaaattct tgtatccgta gcgacctgcc acatgcagta
     7021 tgtacggtga ctcacctacc aggtcacttg ccgtcaaacc gcttactgga agctggtcgt
     7081 accccaagta ggtgacgcta attcgatccg gcccaagccg atacaactcc atcaggtcac
     7141 gccgggtgtt ttccgatatg caaaaaacat ggtcagcgcg gttgaccgcg gctcttttca
     7201 gtcttgctac cggataacca tcgggaaaac tcgccggaaa tctctcttcg atcatgtcgt
     7261 aaacagtcag gacatgcggt actttcgagc ggcacggcga ctgctgcgca taataggtct
     7321 cgtgcacgag atcagggcgc agggcgctgg cgataggccc acacagggcg gcactcacgg
     7381 ccttgatcat tcgcgaagtt ttaggcagcc gtcgcacgtg acagccaact gcaatgccgg
     7441 aatcgatgct cttgagatgc ccgttgatat gaatcggggc aacaatccgg gcttcaacac
     7501 cgctgaaacc gctcatctgc gatgccaacg agcaaacata gcgagagata ccaccgaact
     7561 cctgggcgga aaatatctga cagtcataga taactttcat tgagaaactt ttctattgat
     7621 cagaaatgtt cttcaacatc atctgatggt cgctcagggt tttcgacccc agttgacgac
     7681 gaacccacca catactgaaa agtgatgccc accgaccggt ggcccacccg agcaaacttt
     7741 catcgacagg ttgccgcgac aaaacatgaa gcccatttgc ggtacagaaa cgcaatccca
     7801 acttcgcacc tagaacctcc agggttcttc gttgaaaaaa accgatatgc tgtccggttt
     7861 caaaggtgta gtaccaccat tgatcaggag atggcggcgc gccttggtaa agttcggtgg
     7921 aaaacaatag cgtttgcgca ttgcataaag ccaaggtttc ctcgacaaaa gtgatcggat
     7981 ccgcaagatg ctccatcact tccatcgccg tgaccacgcg gcacgacccg cgctcaggtg
     8041 cgtactcgaa accgggcaca aacaaattgt cacagtattt gtcggaccaa taaaagtcaa
     8101 atcccgcgtc tcgaagcaag cg
//
LOCUS       DAOSLL010000145         8071 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8304_length_8071_cov_6.204716, whole genome shotgun sequence.
ACCESSION   DAOSLL010000145 DAOSLL010000000
VERSION     DAOSLL010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8071
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8304_length_8071_cov_6.204716"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2459
                     /locus_tag="PLB25_08215"
     CDS             <1..2459
                     /locus_tag="PLB25_08215"
                     /inference="COORDINATES: protein motif:HMM:NF023324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3416 domain-containing protein"
                     /protein_id="HOE41596.1"
                     /translation="IIAAVEPQETPPVITEAVEPQETPPVIIAAVEPQETPPVITEAV
                     EPQETPPVIIAAVEPQIDSGDLPIKRLGGEKDTVSADGCKEGQERLVVSLKVRKLGEK
                     TWHESTMTPLANDRWTGEVTVILEAGEPQENPPVIMKAVEPQENAPVVMNAVEPEIDS
                     GCNPNTRIPKDYPRVIIEAVKPQIVGGHFPIKRVVGEKVAVSADVYKEGHDRLAVLLK
                     VRKLGATEWQQSPMTLSANDRWHGEFTADAVGPWEYTIEGYAERYLSWVDEITKKNLP
                     GANLRSELLEGIAILKTSAALAPEADSNRIMGVIWLMESALATGEQQLAIDLGIGNVM
                     AGMMANYPDRSEGYTLAPALPMMVNRPITRFAAWYEFFPRSQSSVAHQHGTLQDSIRR
                     LHEVKAMGFDVVYLPPIHPIGYSFRKGKNNNVVASPGDVGSPWAIGNEHGGHMAIEPQ
                     LGNWNDWDMFVATCHSLGLEIALDYVMNCSPDHPYVAEHPEWFFHRPDGSIKYAENPP
                     KKYQDVYPLNFGTTDQAGLWQEMLKIFLFWVSKGVTTFRVDNPHTKPVPFWEWVIAEV
                     HSKHPEVIFLAEAFTKPKMMRLLAKVGFAQSYTYFTWRTTKPELTEYVSELTRGEMKE
                     YFTGNFFANTPDILPPMLQTGGRPAFIMRAVLAATLSSVYGIYSGYELCENAAIPGKE
                     EYLDSEKYEIKVRDWNAPGNIAAIITRINQIRHASPALQTYNNVLFLTADNPNIIAYA
                     KMTENKSDIILCVVSLDPFRKQHSLVHLPLAEFGIGVDERFQAHDLLSDEHYSWKGPT
                     SYVEFTPDGKVAHIIKLVRL"
     gene            complement(2578..4629)
                     /locus_tag="PLB25_08220"
     CDS             complement(2578..4629)
                     /locus_tag="PLB25_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE41597.1"
                     /translation="MTDDFRLHAWRAIDLLQVPIMTLDLTGYLTSWNRAAENLFGYTP
                     QEAIGQHVLFLYADEADQAETQLAEVMPEHGDTVLEVWRRKKSGENFKAVLTLSLLLD
                     DEQRPVGMVAQLSEIVQALPQLERQKLHSRIIEDSDQGILITDIHERIVSVNAAFSKI
                     TGYQPNEAIGKTTDLLRSGVHDADFRGRVLAAMKGSGPWQGEIVGKRKNGELFPQSVS
                     ISVVRNEVGMVTHAFSIFSDISVLRETEQRMQQLVNFDSLTGLPNRAHFHRLVDLGLA
                     TARRNDSFGALLAIDLRRVDTINETFGHEIGDELLRQVAQRFRQTLRDEDVLARTAAD
                     EFVVALLSIQRREHSSIVAQKLLASLKPGFIIESIVLHVGASIGLSVFPEDGLDTHTL
                     LQFADVAVKRLKAEGDSGYLFYSPEMNQRAKERWQLEGELRRALTSQSLLLHYQPKVS
                     LRNGRIIGAEALIRWNHPEKGMVAPSFFIPIAEETGLIMDIGDWVLDEACRQISAWKT
                     SGINMPMVSVNLSARQFDSLLPARIGAALDRHGLPAACLQLEITESLLVRGTDNVVQI
                     MNDLVAMGLTLSMDDFGTGYSCLAYLKRFPISTLKIDRAFVVGLPHDDSDCAIAQAIV
                     TMGQQLRQEIVAEGVETKAQMHFLRSVGCDQMQGFLFSPAVTADELARMLRDDIRLLL
                     D"
     gene            complement(4622..5617)
                     /locus_tag="PLB25_08225"
     CDS             complement(4622..5617)
                     /locus_tag="PLB25_08225"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOE41598.1"
                     /translation="MNFTSSPVLLHLLADRKLAPGAALLVFDAPAQDALSLLVAHPAF
                     AAFVAEFPCLLGSEQASGLPPELLADLQAAGCRVTDAAAFHRSDTALKPVLPTSALWL
                     DGDWPLAPPQRPTGTQSASRVLALKLLQLVMADAETRDIEAVLRQDPTLSYHLLRLVN
                     SLGMGMTRRITSFSQAILILGRKQLQRWLNLMLFAARSDDYRAPMLLARVVVRARTME
                     LLTKEIGLDLAGQDLAFMAGMFSMLGILFGMSLAELIRPLQLSDVLVDAIIDHQGEIG
                     RLLQLLEYAEQSDAASATDLLAGLRLTAAQFTFASLEACQWMLGIAREMKGSDHD"
     gene            complement(6410..8062)
                     /locus_tag="PLB25_08230"
     CDS             complement(6410..8062)
                     /locus_tag="PLB25_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE41599.1"
                     /translation="MKRRDFLASAGLPLLGCGQTHDIAGGFTGINLERGHLLQAARVW
                     PTPALIRRTDVLIAGGGVAGLAAARALRLQGVEDFALLELEDEAGGNSRGGLTGGQAC
                     PLGAHYLPLPSDDAPEVQDLLEELGLRQRLAGRWVYDERHLCHSPQERLFFNGSWQEG
                     LLPVHEVGQDSIAQYRKFATLVAQLRHQARWSIPASNRPLAPVQQALSAITFEAYLVQ
                     QGFTDGHLRWYLDYCCRDDYGAGLASVSAWAGIHYFASRHGFAAPGLEATERDHLLTW
                     PEGNAWLTRRLAAPLGARLHPGRVVLRVTNLKHAVEIDAFNTATQSIERWHARRAIIA
                     LPIFIAARVLESPPEWLRGAAAQIAYAPWLVANIHIKAALHDRPGAAPAWDNLIHGDR
                     QSAPGLGYVDASHQHLQSVPGATVLTYYRALGDITAAVGSPAQAPGVAGRHMLLGQAW
                     QVWRDAILAELSGPHPDLVGKVTRIDLTRYGHAMAMPLPKVNGQIGLWPPSDKRSKLF
                     KSVQIEIRHERLAFAHSDWAGYSIFEEAFTLGHAAASAGRLL"
BASE COUNT         1732 a         2456 c         2357 g         1526 t
ORIGIN      
        1 tcatcatcgc agcggtcgag ccgcaagaga ctccccctgt tatcacggaa gcggttgagc
       61 cgcaagagac tccccctgtc atcatcgcag cggtcgagcc gcaagagact ccccctgtta
      121 tcacggaagc ggtcgagccg caagagactc cccctgtcat catcgcagcg gtcgaaccac
      181 aaattgacag tggcgatttg ccgatcaagc gcctcggcgg cgaaaaagac acagtcagcg
      241 ccgatggctg caaggaaggc caggaacggc tggttgtttc gctcaaggta cgcaagctcg
      301 gcgagaaaac ttggcatgaa agcacgatga ccccactggc caatgaccgc tggaccgggg
      361 aggtcactgt catcctggaa gcgggcgagc cacaagagaa cccccctgtc atcatgaaag
      421 cggtagagcc acaagagaac gcccctgtcg tcatgaatgc ggtcgagcca gaaattgaca
      481 gtggctgcaa cccaaacacc aggattccaa aagactaccc ccgtgtcatc atcgaagcag
      541 tcaagcctca aatagtcggt ggtcattttc cgatcaagcg cgtggtcggt gaaaaagtcg
      601 ctgtcagcgc cgatgtttac aaagaaggtc atgaccggct ggcggtattg ctcaaggtgc
      661 gcaagctagg tgccactgag tggcagcaaa gcccgatgac actgagcgcc aacgaccgct
      721 ggcatggcga attcacagcc gatgcagtcg ggccttggga atacaccatt gaaggttacg
      781 ccgaacgcta cttgtcgtgg gtggacgaga tcaccaagaa gaacctgccc ggtgccaacc
      841 tcagaagcga actgctcgaa ggcatcgcga tcctgaaaac ctcggccgcc ctggcaccgg
      901 aggccgatag caaccgcatc atgggtgtca tttggctgat ggagagcgcg ctggccaccg
      961 gcgaacagca gttggcgatc gatttgggaa tcggcaacgt gatggccggc atgatggcca
     1021 attacccgga tcgtagcgaa ggctacaccc ttgcaccggc actgccgatg atggtcaatc
     1081 ggccaatcac ccgctttgcc gcctggtacg aattctttcc acgctcgcaa agcagcgtgg
     1141 cgcatcagca tggcaccctg caagacagca tccggcgctt gcacgaggtc aaggcgatgg
     1201 gtttcgatgt tgtttatctg cccccgattc acccgattgg ctacagcttt cgcaagggca
     1261 agaacaacaa cgtggttgcc agccctggtg atgtcggtag cccgtgggcg atcggcaatg
     1321 agcacggcgg gcacatggca atcgaacctc agctcgggaa ctggaacgac tgggacatgt
     1381 ttgtcgccac ctgccacagc ctgggccttg aaatcgcact tgattatgtg atgaactgtt
     1441 cacccgacca cccctatgtg gccgagcacc cggagtggtt ttttcaccgc ccggacggat
     1501 cgatcaaata cgccgagaac ccgccgaaga aatatcagga tgtttacccg ctcaacttcg
     1561 gcaccaccga ccaggccggg ctatggcagg aaatgctcaa gatattcctg ttctgggtca
     1621 gcaagggggt gacaactttc cgagtcgata acccgcatac caagccggta ccgttctggg
     1681 aatgggtgat cgccgaagtc cacagcaaac atccggaggt cattttcctg gcggaagcat
     1741 ttaccaaacc gaagatgatg cggctgctgg caaaagtcgg ttttgcccag tcttacacct
     1801 acttcacctg gcgcaccacc aaacccgaac tcaccgagta cgtgagcgag ttgaccagag
     1861 gcgagatgaa ggagtatttc accggcaact tcttcgccaa tacgccagac atattgccgc
     1921 ccatgctgca aaccggcggt cggccggcct tcatcatgcg cgccgtgctg gcggccacgt
     1981 tgtcgagcgt gtacggcatc tacagcggtt acgagttgtg cgaaaacgcg gctattccag
     2041 gcaaggagga atacctggac tccgaaaaat atgagatcaa ggtgcgcgac tggaacgcgc
     2101 cgggcaacat tgccgccatc attacccgca tcaatcagat tcgtcacgct tcaccggcgc
     2161 tgcaaaccta taacaatgtg ctgtttctga ccgccgataa ccccaatatc atcgcctacg
     2221 ccaaaatgac cgagaacaag tcggacatca tcctgtgcgt ggtcagcctg gatcccttcc
     2281 gcaaacagca ctcccttgtg cacctgccgc tggctgagtt tggcatcggc gttgacgagc
     2341 gcttccaggc gcacgatctg ctgtctgacg agcactacag ttggaaaggc ccaacgtctt
     2401 acgttgaatt cacgccggat ggcaaggttg cacacatcat caaactggtt cgcttgtaaa
     2461 agccgggacg catcccgttg tagcccaatt ttcatcagca aaaatggcag ttcggtgatg
     2521 gaagatgctg ccatcaccgc actgggcatt atttgcgtat cagcctgccg ggcgcgctca
     2581 gtccagtagc aggcggatgt cgtcgcgcaa catgcgagcc agctcgtcgg cagtgaccgc
     2641 cgggctaaac agaaatccct gcatctggtc acagccaacc gagcgcaaaa agtgcatttg
     2701 cgctttggtt tcaacgccct cggcgacgat ttcctggcgc agttgctgac ccatggtgac
     2761 gatggcttgc gcaattgcac aatcgctgtc gtcgtgcgga agacccacga caaaggcccg
     2821 gtcaattttt agcgtggata tcgggaaccg tttcaggtac gccaggcatg aatagccggt
     2881 accaaagtcg tccatcgaca gtgtcagtcc catggccacc aagtcattca tgatctgcac
     2941 cacgttgtcg gtgccgcgca ccagcaggct ttcggttatt tcgagttgca ggcaggcggc
     3001 cggcaagccg tgacggtcca gcgcggcacc tatgcgtgcg ggcagcaggc tatcaaactg
     3061 gcgtgccgaa aggttcaccg acaccatagg catattgatg ccggacgttt tccaggcact
     3121 gatctgacgg caagcctcgt caagcaccca gtcaccaatg tccatgatca acccggtttc
     3181 ttcagcaatc ggaataaaga aactcggtgc caccatgcct ttttcggggt gattccagcg
     3241 aatcagggcc tcggcaccaa tgatgcgccc gttgcgcagg ctaacctttg gctggtagtg
     3301 caacagcagg gattgggagg tcagtgcccg tcgcagctca ccttcaagtt gccagcgctc
     3361 tttggcgcgc tggttcatct cggggctgta aaacaggtag cctgaatcgc cttcggcctt
     3421 gagccgcttg accgcaacat cagcaaactg cagcaacgta tgggtgtcga gcccgtcctc
     3481 cgggaacacc gacaagccaa tgctggcacc cacgtgcagc acgatggact caatgatgaa
     3541 gccgggcttg agcgaggcaa gcagcttttg cgccacgata ctgctgtgct cgcgtctttg
     3601 tatgctgagc agcgccacca caaattcgtc tgcagccgtt ctggccagca cgtcttcgtc
     3661 acgcaaggtc tggcgaaaac gctgggctac ctggcgcaac aactcgtcgc cgatctcgtg
     3721 gccaaaggtc tcgttgatgg tgtcaacgcg ccgcaaatca attgccagca gggcaccaaa
     3781 gctgtcgttg cggcgagccg ttgccaggcc cagatcgacc aggcgatgaa agtgggcccg
     3841 gtttggcagg ccggtcaagc tgtcaaaatt gaccaactgc tgcatgcgct gctcggtttc
     3901 ccgcagcacg ctgatatctg aaaatatcga gaacgcatgg gtcaccatgc cgacttcgtt
     3961 gcgcaccacg ctgatcgata ccgactgtgg aaacagttcg ccgttcttgc gcttgccgac
     4021 gatttcacct tgccaaggcc cggagccctt catggccgcg agaacgcggc cccgaaaatc
     4081 tgcgtcgtgt acccccgagc gcaacaaatc ggtggtcttt ccaatcgctt cgttcggctg
     4141 gtaaccggtg atttttgaaa acgcagcgtt caccgacaca atgcgttcgt ggatatcggt
     4201 gatcaaaatg ccctggtcgc tgtcttcaat gatgcgcgaa tgcagtttct gccgttctag
     4261 ctggggcagc gcctggacga tttcagataa ctgcgccacc atgccgaccg gtcgctgttc
     4321 gtcatcgagc aacagcgaca gcgtgagcac cgccttgaag ttctcgccgg attttttgcg
     4381 gcgccagacc tccagcacgg tatcgccatg ctcaggcatc acctcggcca actgggtttc
     4441 agcctgatcg gcttcgtcgg cgtagagaaa cagcacatgc tggccaatgg cctcctgcgg
     4501 cgtgtagcca aacaggtttt ctgccgcccg gttccaactg gtcaggtaac cggtcaggtc
     4561 aagcgtcatg atcggtacct gcagcagatc gatcgcccgc caggcatgca gccgaaaatc
     4621 atcagtcatg gtcgctacct ttcatttcgc gggcaatgcc cagcatccac tggcaagcct
     4681 ccagactggc gaacgtgaac tgcgccgcag tcaaccgcag cccggcgagc aagtcggtcg
     4741 cgctggccgc atcggattgc tcggcatact ccagcaattg aagcaggcgg ccgatttctc
     4801 cctggtggtc aatgatggca tcaaccagca catcactgag ctgcagcggc cggatcagct
     4861 cggccaatga catgccaaac aaaatgccca gcatggaaaa catgccggcc atgaaagcca
     4921 gatcctggcc agcgagatca agcccgattt ccttggtcag cagctccatg gtgcgggcgc
     4981 gcaccactac ccgggccagc aacattggcg cgcggtagtc gtccgagcgg gccgcaaaca
     5041 gcatcagatt aagccagcgc tgcagttgct tgcggcccaa aatcagaatc gcttgcgaaa
     5101 aactggtgat gcgccgcgtc atgcccatgc ccagcgaatt gaccagccgc agcaaatggt
     5161 aggacagggt tggatcctgg cgcaacaccg cttcaatgtc gcgtgtttct gcgtcggcca
     5221 tgaccagttg caagagcttg agcgccagaa cccgtgatgc cgactgcgtg ccggtggggc
     5281 gctgcggcgg tgccagcggc cagtcgccat cgagccaaag tgccgatgtc ggcaacaccg
     5341 gcttaagcgc cgtatcggag cgatgaaagg ccgccgcatc ggttacccga cagcccgcag
     5401 cctgcaggtc agccagcagt tcaggtggca ggcccgatgc ctgctctgaa ccaagcaggc
     5461 agggaaattc ggcaacaaaa gcggcaaaag ccgggtgcgc taccagcaac gacagcgcgt
     5521 cttgcgcagg cgcgtcaaaa accagcagtg cagcacctgg tgccagcttg cggtcggcca
     5581 gcagatgcaa aaggacgggt gatgacgtga aattcatgga caccttgaaa cgtttggttt
     5641 gaaacgacat attacccccg gcatgccccg aatggtatta agctagattg ctcggttgac
     5701 cgcttgcttt cataggcaag tccttctgaa aatttacttt ttagcttcga tggggttcac
     5761 ccgttagtct ctaacaagtc aagtctttgc ataccatgcg aagaattcaa acattcaaag
     5821 tcattcgccg cgagggcgcg actcctacaa attcagatcg gcgccgactg tttgtgtagg
     5881 agtcgcgccc tcgcgacaaa ggctcatcga ttccggcttg tccggcttag ggcacagcgc
     5941 tacgcatccg attgaaccag tggcaacgtg actgggttcg ttgccccttc ctcgtcggcc
     6001 aggacgacgg gttctgaagc agcagagcgc cgttgtcgcc ccgcgccgct tgccgggcgc
     6061 atactgggcg cagccaagcc acagggctgg cctgcctcaa gccgtccaag cctgcgcagg
     6121 cacggttgga gctgcggctg tcgcccgacc cagccgcgcc gccagcggcc cacttgggcg
     6181 ggttcaactg aggaatccag ggtcaaggcc gtgccacctg gccaagccag caaaagcgcc
     6241 agcgtcacgc gcagcaagtg ctgttgccgt gtctgcaact ggccaggcca tcgggcgggt
     6301 gctgatgaaa gtggcagctt gggtcacaac ggtctgcctc agcgcgagcg acctgctgat
     6361 gctgcacact cgcccacggg cgcgaaggta tggtggatcg gggtctgcct cagagcaggc
     6421 gacctgctga ggctgcagcg tgtcccaggg tgaacgcttc ctcgaagatt gaatagcccg
     6481 cccagtcgct gtgggcaaaa gcaagccgct catggcggat ttcaatttgc actgatttaa
     6541 atagcttgct gcgcttatct gacgggggcc agaggccgat ttgaccattt acttttggca
     6601 gcggcatggc catcgcatgg ccatagcggg tgaggtcgat gcgggtcact ttgccgacca
     6661 aatccgggtg cggtcccgag agttcggcca gaatcgcatc gcgccagacc tgccacgcct
     6721 ggccgagcaa catatgccga cccgccacac ctggtgcctg ggccgggctg ccgacagcgg
     6781 ctgtgatgtc gccgagcgcc cggtaatagg tcaacacagt tgcccccggc acgctctgca
     6841 ggtgctggtg gctggcatcg acataaccca aaccgggcgc gctttgccgg tcgccgtgaa
     6901 tcaggttgtc ccaggccggt gcggcacccg gccggtcgtg caaggctgcc ttgatgtgga
     6961 tattggccac cagccagggt gcgtaagcaa tctgcgcagc agcaccgcgc agccattcgg
     7021 gcgggctttc cagcacccgc gcagcgatga atattggcag cgcaatgatg gcgcggcgcg
     7081 cgtgccagcg ctcaatgctt tgtgttgcgg tgttgaaggc atcgatttcc accgcgtgtt
     7141 tcaggtttgt cactcgcagc acgacacggc caggatgcag gcgcgcgccc agcggcgccg
     7201 ccaggcggcg cgtcagccag gcgttgccct cgggccaagt cagcaggtga tcgcgctcgg
     7261 tcgcctccag gccaggcgcg gcaaagccat ggcggctggc aaagtaatgg atgccggccc
     7321 aggccgacac gctggccagg ccagcaccat agtcgtcacg gcagcagtag tccagatacc
     7381 agcgcaggtg gccatcggtg aagccttgct gcaccagata cgcttcaaaa gtaatagctg
     7441 acagcgcttg ctgtacgggc gctagaggcc tatttgatgc tggaattgac cagcgggcct
     7501 ggtgcctgag ctgcgctacc agcgtggcaa atttccggta ttgcgcgatg ctgtcctggc
     7561 ccacctcatg caccggcagc aagccttctt gccaactgcc gttaaaaaac agccgttctt
     7621 gcgggctgtg gcacaggtgg cgttcgtcat acacccagcg cccggccagg cgctggcgca
     7681 agcccagttc ttcaagcaag tcctgtacct cgggcgcatc gtcgctgggt agcggcaggt
     7741 agtgggcacc cagcgggcac gcctggccac cggtcagacc gccccggctg ttgccaccgg
     7801 cctcgtcttc gagttccagc agggcaaagt cctccacccc ctgcagccgc aaggcgcgtg
     7861 ctgccgccag gccagcgaca ccgccaccgg cgatcagcac atcggtgcgg cggatgagcg
     7921 caggtgttgg ccagactcgc gcggcttgca gcagatggcc ccgctccagg ttgatgccgg
     7981 tgaagccacc ggcgatgtca tgggtctggc cgcaacccag cagcggcaac ccggcgctgg
     8041 ccagaaaatc acgccgcttc acagcgcgac t
//
LOCUS       DAOSLL010000146         8064 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8312_length_8064_cov_4.783369, whole genome shotgun sequence.
ACCESSION   DAOSLL010000146 DAOSLL010000000
VERSION     DAOSLL010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8064)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8064)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8064
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8312_length_8064_cov_4.783369"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="PLB25_08235"
     CDS             complement(<1..188)
                     /locus_tag="PLB25_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE41600.1"
                     /translation="MKTVVVATPSFKQRMLAVREESIVHAVNRLLAEKGFDAMTVDEV
                     AAQVGIAKASLYKHFPGK"
     gene            complement(239..1177)
                     /locus_tag="PLB25_08240"
     CDS             complement(239..1177)
                     /locus_tag="PLB25_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator ArgP"
                     /protein_id="HOE41601.1"
                     /translation="MSTFDPDALECLAAIVEEGGFERAAVRLSITQSAVSQRLRSLEV
                     QVGTVLIVRSRPLKATATGRLLLKHALQLRLLRAYLDRDLKELAPDVAGAAGEDARIS
                     IAVNADSIATWALPALSPLAHQGLGLEIITDDQDFTFEWLREGRVLGCVTTLRSGLRS
                     CKVEPLGCMSYVAVAEPGFAAAHCPQGLSPHNFSRVAFVAFNRKDDLQGEFVADACGL
                     KHVSLRHLYVPSSEGQVQAVLAGWGASVLPELKVREHLRAGTLVNLMPGHALPVALYW
                     HCWNLNSAVLDALTEALKTAAAGVLSPAEVKPKTRQ"
     gene            1390..1782
                     /locus_tag="PLB25_08245"
     CDS             1390..1782
                     /locus_tag="PLB25_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019704046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41602.1"
                     /translation="MALQYTLKEGNYHLYDLSTPTSRVTGEHRLRLKTDTVAIAFDAS
                     TGALREHGSPLRIHCWANNTRRRLRASGALERANDIVVVSGPLPVDEINKCLKIDGYC
                     LDMFTRLASLPHGKVGHRRQGSARHTTH"
     gene            complement(1901..5149)
                     /locus_tag="PLB25_08250"
     CDS             complement(1901..5149)
                     /locus_tag="PLB25_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOE41603.1"
                     /translation="MRFAANEQTLPRLHLIGTLTIVMVLTLALGLFFSWRSAQDHRAS
                     IERVGQTVTALQQSRLQAEMDSVISYLEFTRERTDDMLRKSLREQVDTAVQIAQAIYT
                     RESARQPAAQVKRLIIEALRPVRFYEGRGYYFIDDMQGQFILLPTAPQFEGTTNLDNR
                     DDQGRYIMRGLIEAARRPDGQGYSRYRWYSPDSPKAMADKLAYVRRFAPYDWLIGTGD
                     YTYKWEQAQKQEVIARLRALRFGQSGYFALIDRDGHLVLSQAAPQLEGNHYRDVAPAQ
                     GAALALLVNKGRQGGGFVHYEWPDLKTGAMGGKTGLARLIEPWNWVVVATIQDNELQL
                     NLQQELAQYGVDRLQRWTQWLVPLLLALSMGLLASLSFARWSGKLFAAYHDDIGKKNR
                     TIADSEALFHAVFDNAAVGIAQASLQGQFLQINPYFCRFLGYEHDEMLAPGFDFQQVT
                     WPEDLDADLQHLQRLLAGEDASFSMEKRYIRKDGSIVWGYLAVHLVRAGDGTPQYFIA
                     AVHDMTERKKGEEALSLAASVFTHAREGIMITEVDGTIVNVNDSFTRITGYSREEALG
                     QNPRLLKSGLQSEAYYAEMWQSIEEKGHWYAETWNRRKNGEIYAEMQTISVVRDADGG
                     ARHYVSLFSDITAIKEHERQLEHIAHFDALTGLPNRVLLADRLQQAMWQVQRRGQLVA
                     VAYLDLDGFKAINDRFGHDVGDRVLVGVATRMKAILREGDTLARIGGDEFVAVLVDLP
                     DSDSCLPLLTRLLAGAAEPLLLAENSLQVSASLGVTFFPQADGIDAEQLQRQADQAMY
                     QAKLAGKNRYHVFDAEQDRNARGHFETLEHVRHALKSGELVLHYQPKVNMRSGRVTGS
                     EALIRWQHPQRGLLAPALFLPVIENHALAVDVGEWVIDTALSQVESWHQQGLDLPVSV
                     NVGARQLQQRDFVERLQAILAAHPSVSPACLELEVLETSALEDIAGVSQVIGACHALG
                     VSFALDDFGTGYSSLTYLKRLPVTLLKIDQSFVRDMLDDADDLAILKGVISLAKAFHR
                     EVIAEGVETVAHGTALLQLGCELAQGYGIARPMPAADLLAWAASWKPDAAWG"
     gene            complement(5320..6291)
                     /locus_tag="PLB25_08255"
     CDS             complement(5320..6291)
                     /locus_tag="PLB25_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOE41604.1"
                     /translation="MQVNTRAALGQLSDMDIRLLRVFKSVVDCGGMAAAELELNIGTS
                     TISRHIKDLETRLGLTVCRRGRAGFALTPEGGQIYAETLRLLSSVDAFRSRVDEIHQR
                     MGGELHIAVFDNTASNPQAHIGEAIARFTQAAPDVSLHLHVAPLNSIERGVIDGQFQV
                     GIIPGHRSSDALHYDELFDETMLLYCGQSHPLFAADAALEPNNWEALRGHRFAGLGYH
                     SPNMELSQQVRLTRAATGYDQESIATLILSGRFLGFLPDHYAERFVQLGRMRAVQPKV
                     ISYRCSFFCILRASPQSARVTRAFQACLLASHLHITGNHNEIGLQPL"
     gene            6418..7766
                     /locus_tag="PLB25_08260"
                     /pseudo
     CDS             6418..7766
                     /locus_tag="PLB25_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020702856.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
     gene            7770..>8064
                     /locus_tag="PLB25_08265"
     CDS             7770..>8064
                     /locus_tag="PLB25_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HOE41605.1"
                     /translation="MYLPAHFSQTDPAALHAVMRQYPLATLVTQADGEPVADELPFVL
                     DAELGPHGTLNAHVARANPLWQVHPAQRSVLVVFRGPQAYVSPSWYPGKAEHGR"
BASE COUNT         1628 a         2568 c         2378 g         1490 t
ORIGIN      
        1 tccttgccgg gaaagtgttt gtacaaactg gctttggcaa tgcccacctg cgctgccacc
       61 tcatcgaccg tcatcgcatc aaaacccttt tcggccagca aacggttaac cgcatggacg
      121 atcgattctt cgcgcaccgc cagcatgcgc tgcttgaacg aaggtgtggc aacaacaact
      181 gttttcatgc cgacatctta gccgaggtct tcaaaatctg ggcgctgcgg ctgttttgtt
      241 actgcctggt cttaggcttg acctcggccg gtgacaaaac cccggcagcg gcggttttca
      301 gggcctctgt cagcgcgtcc agcaccgccg aattcaggtt ccagcagtgc cagtacagcg
      361 ccaccggcaa ggcatggccg ggcatcaggt tgaccagcgt gccagcgcgc aaatgctcgc
      421 gcactttcag ttcgggcagc acgctggcac cccagccagc cagcaccgcc tgcacctgcc
      481 cttccgagct gggcacatac aaatggcgta gcgacacatg cttgaggccg caggcatcgg
      541 ctacaaactc gccctgcaaa tcgtccttgc ggttgaaagc gacaaacgcc acgcggctga
      601 aattgtgcgg actcaggccc tgggggcaat gggcagccgc aaatccgggc tcggccaccg
      661 ccacatacga catgcaacca agcggctcga ccttgcaact gcgcaagccc gagcgcagcg
      721 tggtcacgca gcccagcacc cggccttcgc gcagccactc aaaggtgaaa tcctggtcat
      781 cggtgatgat ctccagcccc agaccctgat gtgccagcgg gctgagggcc ggcagcgccc
      841 aggtggcaat gctgtcggcg ttgacggcaa tggagatacg cgcgtcttcg ccggccgcac
      901 cggccacgtc aggagccagt tctttcaggt cgcggtccag atacgcgcgc agcagacgca
      961 actgcaacgc atgcttgagc agcaagcgtc cggtcgcggt ggccttgagc gggcgactgc
     1021 ggacaatcag caccgtgccg acctgcactt ccagcgagcg cagccgctgc gacacggcag
     1081 actgggtgat cgacagccgc accgcggccc gctcaaaacc gccttcttcg acaatcgcag
     1141 caaggcattc cagcgcatca ggatcaaaag tgctcatgtc aaaaataaga atggcgaatg
     1201 aaaaaaaggc agtttaatca gttcggcaac atggcaaggc agggtgtcga attggtcatg
     1261 cagacggttt tttgaaaaaa tgcatgcggg ttttcccgta ataccgatga aaaagagacg
     1321 tattgacccc tgtttgccct ttagcacggg gcagtgtcct gcaaaatgtt cgcaacaaca
     1381 ggagattcca tggcattgca gtacacgctc aaagaaggta actaccactt gtatgacctg
     1441 agcacgccca ccagccgggt caccggcgag caccggctgc gcctgaaaac cgacaccgtg
     1501 gcgatagcct ttgatgccag cactggcgct ttgcgcgagc atggcagccc gttgcgcatc
     1561 cattgctggg ccaacaacac ccgccgtcgc ctgcgcgcca gtggtgcact ggagcgcgcc
     1621 aatgacatcg tggtggtgtc gggtccgctg ccggtggacg agatcaacaa gtgcctgaaa
     1681 attgatggct actgtcttga catgttcacc cgcctggcca gcctgccgca cggcaaagtt
     1741 ggccaccgcc gccaggggtc agcgcggcac accacgcact gatggctgga ctggcgcgac
     1801 ccagctcgtt cagccctgtg ttgattggtg taaccgaagc ctgccctcaa gcctgcaggg
     1861 gaaaccggac ccgccgggcg atcagtcgtt tgcccatcaa ctagccccac gcagcgtcag
     1921 gcttccagct tgctgcccag gcaagcaaat cggcggctgg catcggccgg gcgatgccat
     1981 agccttgcgc cagttcgcaa cccagttgca gcagcgcggt gccgtgggca acggtttcca
     2041 cgccctcggc aataacctcg cggtggaagg cctttgccag actaatcacg cctttcagaa
     2101 tcgccaggtc gtcggcatca tcgagcatgt cgcgcacaaa gctctggtcg attttcagca
     2161 gggtcaccgg caggcgcttg aggtaggtga gcgacgaata accggtgcca aagtcatcga
     2221 gcgcaaaact cacacccagt gcatggcaag cccctatcac ctgcgacacc ccggcaatgt
     2281 cttcgagggc gctggtttcc agcacttcaa gctccaggca agccggactg acactggggt
     2341 gcgccgccaa aatggcctgc aggcgttcaa cgaaatcgcg ctgctgcaac tggcgcgctc
     2401 cgacattgac gctgaccggc aaatccagcc cctgctggtg ccagctttcc acctggctca
     2461 gggcggtgtc gatgacccat tcaccaacat cgaccgccag cgcatggttt tcaatcaccg
     2521 gcaaaaacag cgccggtgcc agcaggcccc gctgcgggtg ctgccagcgg atcagcgcct
     2581 cggaacccgt cacccggccg ctgcgcatgt tcaccttggg ctggtaatgc agcaccagct
     2641 caccgctttt cagcgcatgg cggacgtgct ccagggtctc aaaatggccg cgcgcgttgc
     2701 ggtcctgctc tgcgtcaaag acgtggtagc ggtttttgcc ggccagcttg gcctggtaca
     2761 tggcctgatc ggcctgtcgc tgcaattgct cggcatcaat gccgtcagcc tgcgggaaaa
     2821 acgtcacccc caggctggcc gacacctgca ggctgttttc agccagcagc agcggctcgg
     2881 cggcgccggc caacaaccgg gtcagcaacg gcaggcaact gtcgctgtca ggcaggtcca
     2941 ccagcaccgc cacaaactcg tcgccaccga tgcgcgccag cgtgtcaccc tcgcgcagga
     3001 tcgccttcat gcgagtggcc acccccacca gcacccggtc accgacatca tgtccaaagc
     3061 ggtcattgat ggccttgaaa ccatcaaggt ccagataggc caccgccacc aactggccgc
     3121 gccgctgcac ctgccacatg gcttgctgca ggcggtcagc cagcagcacc cggttgggca
     3181 gaccggtcag cgcgtcaaaa tgggcaatgt gctccaactg acgctcatgc tccttgatgg
     3241 cggtgatatc ggaaaacagc gacacatagt gccgcgcgcc gccatcggca tcgcgcacca
     3301 cgctgatggt ttgcatctcg gcataaattt cgccattttt gcggcggttc caggtttctg
     3361 cgtaccagtg gcccttctct tctatgcttt gccacatctc tgcgtaatag gcctcggact
     3421 gcaggcccga cttgagcaga cgcgggttct ggcccagcgc ttcttcacgg ctgtagccgg
     3481 tgatgcgggt aaacgaatcg ttgacgttga caatggtgcc gtcaacctcg gtgatcatga
     3541 tgccctcacg cgcatgggtg aacacgctgg ctgccagaga caaggcctct tcgccctttt
     3601 tgcgctcggt catgtcgtga accgcagcaa tgaagtactg cggtgtgccg tcgccagcgc
     3661 gcaccaggtg caccgccaga tagccccaga caatgctgcc atctttgcgg atgtaacgct
     3721 tttccatgct gaagctggca tcctcgccag ccagcaagcg ctgcaggtgt tgcagatcgg
     3781 catccaggtc ttctggccag gtcacttgct gaaagtcaaa tcccggcgcc agcatctcgt
     3841 catgctcata acccaggaag cgacaaaaat aggggttgat ctgcagaaat tgcccctgca
     3901 gcgatgcctg ggcgatgccc acagcagcgt tgtcaaagac cgcgtgaaac agagcctcac
     3961 tgtcggcaat ggtccggttt tttttgccga tgtcgtcgtg gtaggcggca aacagcttgc
     4021 ctgaccagcg ggcaaagctc agcgatgcca ataaccccat gctcagcgcc agcagcagcg
     4081 gcaccagcca ttgcgtccag cgctgcagcc ggtcaacgcc gtactgtgcc agttcctgct
     4141 gcaggttgag ttgcaattcg ttgtcctgaa tggtcgctac cacgacccag ttccagggtt
     4201 cgatcaagcg cgccagaccg gtcttgccac ccatcgcccc ggtcttgagg tcgggccact
     4261 cgtagtgcac aaaaccaccg ccctgccgac ccttgttcac cagcagggcc agcgctgcac
     4321 cctgcgccgg ggcaacatcg cggtagtgat tgccttcgag ctgcggggcc gcctgcgaca
     4381 gcaccaggtg gccgtcgcgg tcaatcaggg caaaatagcc gctctgacca aaacgcagtg
     4441 cccgcaagcg cgcaatcacc tcctgctttt gcgcctgctc ccacttgtag gtgtagtcac
     4501 cggtgccaat cagccagtca tacggcgcaa agcgccgcac ataggccagt ttgtcggcca
     4561 tcgccttggg cgaatcgggt gagtaccagc gatagcgtga atagccctgc ccatcgggtc
     4621 ggcgcgctgc ctcaatcagg ccacgcatga tgtagcggcc ctggtcgtcg cggttgtcca
     4681 gattggttgt gccctcgaat tgcggtgccg tcggcaacaa aatgaattga ccctgcatgt
     4741 cgtcaatgaa gtaataaccg cgtccctcat aaaagcgcac cgggcgcaag gcttcgatga
     4801 tcaggcgttt gacctgcgcc gccggctggc gtgccgactc gcgggtgtag atggcctggg
     4861 caatctggac tgcggtgtca acctgttcgc gcaggctttt gcgcagcatg tcgtcggtgc
     4921 gctcgcgggt gaactcaagg tagctgatga ccgaatccat ttcagcttgc aagcgtgact
     4981 gctgcagcgc ggtgacggtt tgcccgaccc gctcgatcga ggcccggtga tcctgcgcgc
     5041 tgcgccacga gaaaaacaga cccagcgcga gtgtcagcac catcacaatg gtcagggtgc
     5101 cgatcaggtg cagccgggga agggtctgct cattcgctgc aaaacgcaca atggtgctcc
     5161 tgctcttgaa gtcgcggcgc aacgggttcc tatgccagag caattcattc tacgcattta
     5221 gcccggctgg gtgtacgttt ggcaaagcct tttcaatgca tggagatcga ccccgcagcg
     5281 ctcttcatgc tactaggtta atagctgcca gcgcttgttt cataagggct gcagcccgat
     5341 ttcattatga ttgccagtga tgtgaagatg gctggccagc aaacacgcct gaaacgcgcg
     5401 cgtcaccctg gcgctttgcg gcgaggcccg caaaatgcaa aaaaaactgc agcggtagct
     5461 gatcaccttg ggctgcaccg cccgcatgcg gcccagttgc acaaagcgct cggcgtaatg
     5521 gtctggcaaa aagcccagaa accggcccga cagaatcagc gtggcaatcg actcctggtc
     5581 gtaaccggtg gcagcgcggg tcaggcgcac ttgctgcgac agctccatgt tgggcgagtg
     5641 gtaacccagg ccagcaaagc ggtgcccgcg cagcgcctcc cagttgttgg gctcaagcgc
     5701 cgcatcagca gcaaacagcg ggtggctctg cccgcaatac agcaacatgg tttcgtcaaa
     5761 cagctcgtca tagtgcagcg catccgaact gcggtggccg ggaatgatgc cgacctgaaa
     5821 ctggccgtcg atgaccccgc gctcaatgct gttgagcggg gcgacatgca ggtgcaggct
     5881 gacatcgggt gcggcctggg tgaaccgcgc aatcgcctcg ccaatatggg cctgcgggtt
     5941 gctggcggta ttgtcaaaca ccgcaatgtg cagttcgccg cccatgcgct ggtggatttc
     6001 atcaacccgg ctgcgaaagg catcgacgct tgagagcagg cgcagcgttt cggcgtaaat
     6061 ctgcccgccc tcgggggtca gggcaaaacc ggcccggcca cggcggcaaa ccgtcaggcc
     6121 aaggcgggtt tccaggtcct tgatgtgccg actgatggtc gatgtgccaa tgttgagttc
     6181 aagttcggca gccgccatgc cgccgcaatc gaccacgctt ttgaacaccc gcagcaggcg
     6241 gatgtccatg tcgctcaact ggcctaaggc ggcgcgggtg tttacttgca tagatttgca
     6301 accaagcagt gatagtcagg caatcataag agtaagacga caccgcacaa tcgccccttt
     6361 gccgccctgc gtaaggaaat ggtcaaaaac agcaaccttg ccccactgga gaaccccatg
     6421 aacctgcctg acaccaacgt cctgacccag cccggaaccg atgccgcctg gctggagccg
     6481 cactggatgc cctacaccgg caaccgcaac ttcaaggcca acccgcgcat gatggtcagc
     6541 gccgaaggct gctactacac cgacgttgac gggcgcaaga tttttgatgg cctgtcgggc
     6601 ctgtggtgct gcggacttgg ccatggccgc gccgagatca ccgaagcggc acggcaacag
     6661 ctcgccacgc tggactattc gccagccttc cagtacggcc acccgctgtc gtttgcgctg
     6721 gccaacaagc tcaaggaact gacccccgag gggctggact atgtgttttt caccggctcc
     6781 ggctccgagt cggccgacac ctcgctgaag ctggcgcggg cctactggcg cgccaagggc
     6841 caaagcagca agagcttgct gattggccgc gaaaagggct accacggggt caactttggc
     6901 ggcatttcgg tcggtggcat cggtgcgaac cgcaagacct ttggtcaggg tattgccgct
     6961 gaccacctgc cgcacaccca gttgcccggc aacacctttg tgcgcggcat gcctgaacaa
     7021 ggtgccgaac tggccgacga actgctgcgg ctgattgcgc tgcacgatgc cagcaacatt
     7081 gcggcggtga tcgtcgagcc aatgtccggc tcggccgggg tgatcattcc gcccaagggt
     7141 tacttgcagc gcctgcgcga catttgcacc gcccacaaca tcctgctgat ttttgacgaa
     7201 gtcatcaccg gccttggccg catgggcggc tacaccggcg ccgaggtgtt tggcgtgacc
     7261 ccggacatca tgaacctggc caagcagatc accaatggcg cgcaaccgct cggcgcggtg
     7321 ctggccaaaa aggagattta cgacaccttc atggcgcaag gcgggccaga gtacctgctg
     7381 gaatttgccc acggctacac ctattcggca cacccggtgc ctgcgcggtg ggtctggcca
     7441 cgctggacat tctggtgcgc gaaaacatgg ttgaccgggt caaggcgctg gcaccgcatt
     7501 ttgagagcgc ggtgcacagc ctcaaggggg ccaggcacat cagcgacatt cgcaactacg
     7561 gtctggccgc cggctttacc attgatgcgg cacccggtga accggccaaa cgaccctacc
     7621 agatagccat ggcgatgctg caaaagggtt tttatgtgcg ttacggcggc gacaccatcc
     7681 agctcgcacc gcccttcatc agcacgccag cgcagatcga cagcctggtc aacgcgctgg
     7741 gcgagtgcat caacgcgacc gcctgacgca tgtacctgcc agcccatttc agccagaccg
     7801 acccggcagc gttgcacgcg gtgatgcgcc agtacccgct ggcgactttg gtgacgcagg
     7861 ccgatggcga gccggtggcc gatgaactgc cctttgtgct cgatgccgaa ctggggccgc
     7921 acggcaccct caatgcgcat gtggcgcgcg ccaacccgct gtggcaggtg cacccggcac
     7981 agcgtagcgt gctggtggtt tttcgcgggc cacaggctta tgtgtcgccg tcgtggtatc
     8041 cgggcaaggc cgagcatggt cggg
//
LOCUS       DAOSLL010000147         8059 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8321_length_8059_cov_4.340205, whole genome shotgun sequence.
ACCESSION   DAOSLL010000147 DAOSLL010000000
VERSION     DAOSLL010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8059
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8321_length_8059_cov_4.340205"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="PLB25_08270"
     CDS             complement(<1..841)
                     /locus_tag="PLB25_08270"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41606.1"
                     /translation="MHTFFCLILALCCACCPPLLAAQAATAPLLRIDSGSHSAPIRAL
                     AVDAAGRYAVTAAEDKTARVWDLGDGRLLQTLRPPAGSGNDGKLFAAAITANGALIAV
                     GGWSADNDVYLFERASGRLLQRITGLPNIITHLSFSPDGKLLLVSLWGKHGVRLFEGN
                     SDWQKLREADSDSSYQGDSYAASFSADASRLVTTSQDGLVRLYDVRDQRLKLLQSVRP
                     VAAAAAFGVAFAPDGKHIALSFSDRQAVLVLDARTLQTAYLPSLTGIDNGSLTALAWS
                     ADGR"
     gene            complement(843..2120)
                     /locus_tag="PLB25_08275"
     CDS             complement(843..2120)
                     /locus_tag="PLB25_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOE41607.1"
                     /translation="MVQTVNFRGTSKRCWCVKRGGLVLGTALLLLACAAPDGRVEAPA
                     AATLVATSTPPPAAPPVAFAHNEAVLMAARDLFGKAQISADKPISVVIDPLVDGSTGL
                     QSVATEAIAKQLVNVVSSEFPKFQIKALTAANVAELPLVFIGTFTPINLQGKGEGERD
                     AYRICFALADLKTGKLVSKGFARSQTAGVDPTPLPYFRDAPLWVNDKVVEGYIKTCQG
                     SKVGDPINPDYLDKVLSVVGIAEATSAYNNKQYREALAQFSTLLRSKAGDQTRVHTGV
                     YLSNVKLGRRTPAMKNFAEIAQRGLNADRLAVKFNFQPGSVTLARSDSPYDLWLKTLA
                     SQSALTAKASAGCFEISGHTGRGGSEAMNERLSLQRAEYVKQRLIAENGDLARRINAR
                     GYGSRQALIATGRDDESDALDRRIEFRHAPCQV"
     gene            complement(2232..2357)
                     /locus_tag="PLB25_08280"
     CDS             complement(2232..2357)
                     /locus_tag="PLB25_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41608.1"
                     /translation="MPIPVRLDQISACALTGIIRWNTCNTKTGVRSPATRTGIFC"
     gene            complement(2380..3978)
                     /locus_tag="PLB25_08285"
     CDS             complement(2380..3978)
                     /locus_tag="PLB25_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOE41609.1"
                     /translation="MPNLLRALFIRSSFAICLILGQMAMMAVAVAADSPLLQVPRHAL
                     VIGNSRYVVGALTNPGNDARSIAERLSQAGFSVTLKLDTGHRDLTEAIRSFSDTLSRD
                     KGVGVFYYAGHGVQLNWRNFLVPVDARIRGKADIQSQAVDLGLLLDGLGRARNAMNIV
                     ILDACRNNPFGEDFRVDDKGLSQLDAPTGTLLAFATAPGNTAEDGIGSHGLYTDNLLK
                     EMQTTGAAVEDVFKRVRLSVRRGSQGSQIPWESTSLEADFSFLPGQTRRDAAKEFEAD
                     LNAWQQLRSADSTDQLDAFIRQRPSGKFAEMAQFRLDQLLAANGEKPVRARAGSAEIC
                     LPGTRQLASYTGQSVPYKVGERYGYRRLNMLSQTEVMRFEQTVAKVAGDEVFFNDGNT
                     VTDLFGNNVRAPDGRKWTPYQFFIADYQVGKRWPAQFLVTLPDGRQHNTSFELRVAGR
                     ERITLPAGTFDALRIEARGTNLSTGDLLERTAWVMPEKMRGFLAQESVIRRGGKLIEG
                     ERTELTAYSLPASPFNGRRFRSGY"
     gene            4134..5078
                     /locus_tag="PLB25_08290"
     CDS             4134..5078
                     /locus_tag="PLB25_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HOE41610.1"
                     /translation="MHLIIPYASGLSDGCLAAQASLKLPHLQRLLDRCHPSPPDAGDA
                     LSLSPPHERVLARAIGLGLADGQIAWAALRAREHPQLPDGGAWAFITLCHWQVHTHHI
                     AMSHLPLPELTQAESAALLAAMRPYFKEDGIALFDDQPGRWLAQAELFADLACAAPDR
                     AAGRNLAHWMPGSVKAAPLRRLQNEMQMLLYTHPVNDARQARGVTPVNSFWLSGTGAL
                     PSGYRMPDASAQPTLVNTLRAAALADDWLAWTQAWQTLDATEIRLMLQHLACGQPVQL
                     TLCGERSSQCWHGQKLSAWQSFKHLFASQPLCTVLEKL"
     gene            5118..6473
                     /locus_tag="PLB25_08295"
     CDS             5118..6473
                     /locus_tag="PLB25_08295"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HOE41611.1"
                     /translation="MPRHLLYLIVFIEGFCSLGAEVIALRRLVPYVGSAIVVTAPTIG
                     LFLLALALGYASGARVGARFTAVVARNFLISGLLAGLGLARLTVDGLFGLVQPGWLAY
                     LLFMGGVLCPLAWLLGQTVPVLTNLMKHARAGEASGYALYWSTLGSFLGSVSLSLGVM
                     QWLGVSAAVLCCAVLLALGSLLLEGQAQPSWQAWAKVLSVGLAASAINLMPASLVQDT
                     AYADYAIKPLTRPGQVEPRAFLINNSVASIMDSSKPARYARYIEHLRHIILDELALEG
                     RDILVLGAGGFNLSHQELTNHYTYVDIDAKIRTIAEADFLKEPIRGAFVVDDARHFVS
                     STTQRFDAVVVDAFSSHTSIPSHLVTREFWQDTRRTLKPQGVLLINLILDGRLESAYA
                     RNLLGTIESVFGRCAVEVLHKSAPLSNVIVSCYQTSHAAPTSPYTDERNAADVDRALS
                     Q"
     gene            6522..>8059
                     /gene="recJ"
                     /locus_tag="PLB25_08300"
     CDS             6522..>8059
                     /gene="recJ"
                     /locus_tag="PLB25_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HOE41612.1"
                     /translation="MKLVVRDAPPRTVWTLEQAGIHPLLAQLYAARGVQDPQELDAAL
                     ARLLAPATMLGTAAAARLLADAMAQDKKFCIVADYDCDGATACAVAVRGLRLLGARHV
                     DYIVPDRVQDGYGLTPPISERVKTSGADLLITVDNGIASFDGVTRARDLGLQVLVTDH
                     HLCASLHGQVALPDADVIVNPNQPGCGFQSKSIAGVGVMFYVLLALRAELRLRGSLNE
                     QTQPRLDALLPLVALGTVADVVRLDANNRRLVAQGLKRVRALAMPAGLAALFVAASRP
                     ATTATTFDFGFALGPRINAAGRLSDMTLGIECLLTDDAGRALELAKTLDGINRERREI
                     ESHMRDQALAVAEALFEDASHAPPAICVFDAGFHEGVVGIVASRIKDKFHRPSFVFAS
                     SGAVGHGHELKGSGRSIAGFHLRDALDLVAKRYPGVLLRFGGHAMAAGCTIDEAHFET
                     FERGLNEVAQEWLDVATLTRRLDTDGPLKPEYRRPDLVDALHHEVWGQGFAAPTFSEE
                     LEVVS"
BASE COUNT         1472 a         2480 c         2528 g         1579 t
ORIGIN      
        1 cacggccatc ggctgaccag gccagcgctg tcaggctgcc gttgtcaatg ccggtgaggc
       61 ttggcaggta agcggtttgc agcgttctgg catcgagtac cagcaccgcc tgacggtctg
      121 aaaaactcag cgcaatgtgc ttgccatcgg gcgcaaaggc aacgccaaat gccgcggcag
      181 cagcgaccgg gcggacactt tggagcagct tcaggcgctg atcacggaca tcgtacaaac
      241 gcaccagacc gtcctggctg gtggtcacca ggcggcttgc gtcggcggaa aaactggcag
      301 cgtaagagtc gccctgatag ctgctgtcgc tgtcggcttc acgcagcttt tgccagtccg
      361 agttgccttc aaacaggcgc acgccgtgct ttccccacag gctcaccagc agcagcttgc
      421 cgtctggaga aaaactcaag tgggtgatga tgttgggcag gccggtaatg cgttgcagca
      481 gacggccgct ggcgcgctcg aacagataaa cgtcgttgtc ggctgaccag ccaccaacgg
      541 caatcaaggc accattggcg gtgatggcgg cggcaaacaa tttgccgtca ttgccgctgc
      601 ctgcgggggg ccgcagcgtc tgcaacaaac ggccatcacc caagtcccac acccgggcgg
      661 tcttgtcttc ggcagccgtc accgcgtagc gaccggcggc atcgacggcc aacgcccgaa
      721 ttggcgcgct gtggctgcca ctgtcaattc gcagcagcgg cgcagttgcc gcctgggccg
      781 ccagcagcgg cgggcaacag gcgcagcaaa gcgccagtat caggcagaaa aaggtgtgca
      841 actcagacct gacaaggggc gtgcctgaac tcgatgcggc ggtccagcgc gtccgactca
      901 tcgtcgcggc cggtggcaat caaggcctgc cgcgagccgt agccgcgtgc attgatgcgc
      961 cgggccaagt cgccgttttc tgcaatcagg cgctgcttga cgtattcagc gcgttgcagt
     1021 gaaagccttt cgttcattgc ctcggagcca ccgcgcccgg tgtggccgct gatctcgaag
     1081 cagcctgcgc tggccttggc ggtcaaggcc gattggctgg ccagtgtctt gagccacagg
     1141 tcataggggc tgtcgctcct ggccagggtg acactgcctg gctgaaagtt gaatttgacc
     1201 gccagccgat cagcgttcag gccacgctgg gcaatctcgg caaagttttt catggctggt
     1261 gtgcgccgcc ccagtttcac attggagagg taaacgccgg tgtggacgcg ggtctggtcg
     1321 ccagccttgc tgcgcagcag ggttgagaac tgagccagcg cctctctgta ttgtttgttg
     1381 ttgtaggcgc tagtggcctc ggcgatgcca accaccgaca gcaccttgtc gaggtaatcg
     1441 gggttgatcg gatcgcccac cttgctgccc tggcaagtct tgatgtagcc ttcaaccacc
     1501 ttgtcattga cccacaaggg tgcatcgcga aaatagggca ggggggtcgg atcgaccccg
     1561 gcagtctgag atctggcaaa gcccttgctg acaagcttgc cggttttcag gtctgccagg
     1621 gcaaaacaga ttcggtaggc atcacgttca ccctcgccct tgccttgcag gttgatgggc
     1681 gtgaaagtgc caatgaacac caatggcagc tcggccacat tggcagcggt gagcgccttg
     1741 atctgaaatt tcgggaactc gctgctgacc acattgacca gttgcttggc aatcgcctcg
     1801 gttgccaccg actgcaggcc ggtgctgccg tccaccagcg gatcaatcac cacactgatg
     1861 ggtttgtcgg cgctgatctg cgccttgcca aacaggtcgc gggccgccat cagcacggct
     1921 tcattgtggg caaacgccac tggcggtgcc gctggtggcg gggttgaggt cgccacaagt
     1981 gttgcagcag caggggcttc aacccggcca tcgggggccg cacaggccag cagcaacagc
     2041 gccgtaccca gcaccagacc accgcgcttg acgcaccagc aacgctttga agtcccccga
     2101 aaattgaccg tctgaaccat ggcgcactcc ttcaagtgtg ttttcaaatc tgacccgaac
     2161 cagcaacatc gtccgaaccg tctcgttgac cgcagtacag acgtttggtc aagccaggag
     2221 cgacgcaaac tctagcagaa gatgccggtg cgcgtggcgg ggctgcgaac acctgttttg
     2281 gtattgcagg tgttccacct gatgatgccg gtcagtgcgc aggcgctgat ctggtcaagc
     2341 cgaacgggaa taggcatggg ctttcaccag cgcacccggc tagtagccgg accggaagcg
     2401 acggccattg aacgggctgg caggcagtga ataagcggtc agttcggtac gttcgccctc
     2461 gatcagcttg ccgccgcgcc gaatcacgct ttcctgcgcc agaaagccgc gcattttttc
     2521 cggcatcacc caggcggtgc gctctaacag gtcgccggtg ctgagattgg tcccgcgcgc
     2581 ctcgatccgc aaggcatcga aggtgccagc gggcaaggtg atccgctcgc gcccggccac
     2641 gcgcagctcg aagctggtgt tgtgctgccg accatcgggc agggtgacga gaaactgcgc
     2701 tggccagcgt ttgccgacct ggtagtcggc gataaagaac tgatagggtg tccatttccg
     2761 accgtccggt gcgcgcacat tgttgccgaa caggtcggtt acggtgttgc cgtcgttgaa
     2821 gaatacttca tcaccggcca ccttcgccac cgtctgctca aatcgcatca cttcggtctg
     2881 gctgagcatg ttcagccgcc ggtagccata acgctcgcca accttgtaag ggaccgattg
     2941 cccggtgtag cttgccaact gtctggtgcc aggcaggcaa atctcggccg atccggctcg
     3001 ggcgcgaacc ggcttctcgc cgttggcagc caacagttgg tcgagccgga actgggccat
     3061 ctcggcgaat ttgccgctgg gacgctgtcg aatgaaagcg tccagttgat cagtggaatc
     3121 ggcagagcgc aattgctgcc aggcgttcag gtcggcctcg aattccttgg ctgcatcgcg
     3181 tctggtctgg cccggcaaga aagaaaaatc tgcttccagc gaggtgcttt cccagggtat
     3241 ctggctgccc tgcgaaccgc gccggacgga cagccggacc cgcttgaaga catcttcgac
     3301 ggcagccccg gttgtctgca tttccttgag caagttgtcg gtgtacaggc cgtgactgcc
     3361 gatgccgtcc tcggccgtgt tgccgggcgc ggtggcaaag gccagcaggg tgccggttgg
     3421 ggcatcgagt tgcgacaagc ccttgtcatc aacgcgaaaa tcctcgccaa aggggttatt
     3481 gcggcaagcg tccaggatga cgatgttcat cgcattgcgg gcgcggccaa ggccatcgag
     3541 caacaggccg agatcgaccg cctgcgactg gatatcggct ttgccgcgaa tcctggcatc
     3601 caccgggacc aggaagttgc gccagttgag ctgcacgccg tggccggcgt aatagaaaac
     3661 gccgacacct ttgtcgcggc tcaaggtatc gctgaagctg cggatggcct ctgtcaaatc
     3721 gcggtgtccg gtgtcgagct tcagcgttac cgagaaaccg gcctggctca ggcgctcggc
     3781 aatcgaccgg gcatcgttgc cggggttggt cagcgcgccg accacatagc ggctgttgcc
     3841 gatcaccagg gcatggcggg gcacctgcaa taaggggctg tcggctgcga cggcgacggc
     3901 catcatggcc atttgaccca gaatcaggca gatggcaaag cttgagcgga tgaatagagc
     3961 gcgaaggagg tttggcatgg tgagcaagaa taattcgatg cgactaattc attatctgcg
     4021 cggatcaacg cgtagggaaa tctccgcgca actggagcta ccagccagca ggggcatccg
     4081 ctgactttgg atgactttca atggccccgc gaaagcacag acacaataca ggcatgcacc
     4141 tgattattcc ttacgcatct ggtctgtccg atggctgcct tgcagcgcaa gcaagcctga
     4201 aactgcccca tttgcaacgg ctgcttgacc gctgccaccc gtcgccaccc gatgctggcg
     4261 atgcgttgag cctgtcgccc ccgcatgagc gtgtgctggc gcgcgcaatt ggccttggcc
     4321 tggcagatgg ccagatcgcc tgggctgcgc tacgcgcccg tgaacacccc caattgcctg
     4381 atggcggtgc ctgggctttc atcaccctgt gccattggca ggtgcatacc caccatatcg
     4441 ccatgagtca cctgccgctg ccagaactga cgcaggccga gtcggctgcg ctgctggctg
     4501 ccatgcgccc ctatttcaaa gaggacggta tcgccctgtt tgatgaccag ccggggcgct
     4561 ggctggcgca ggccgagctg tttgccgact tggcctgcgc cgcgccagat cgggctgcgg
     4621 ggcgcaacct ggcgcactgg atgccgggct cagtcaaggc cgcgccactg agacggctgc
     4681 aaaatgaaat gcagatgctg ctctacaccc acccggtcaa cgatgcccgc caagcgcgcg
     4741 gtgtgacacc ggtcaactcg ttctggctca gcggtactgg cgctctgcca tctggctacc
     4801 gcatgcccga tgccagcgcg cagccgacac ttgtcaacac cttgcgcgcg gcggcgctgg
     4861 cggatgactg gctggcctgg acgcaagcct ggcagacgct ggatgccacc gaaatcaggc
     4921 tgatgctgca gcacctggcc tgcggccagc cggtgcaact caccctgtgc ggtgagcgca
     4981 gcagccagtg ctggcacggc cagaagctct cggcatggca gtcattcaag catctttttg
     5041 cctcccagcc cttatgtact gtgcttgaga agctatgagt tttgtaacat tttcagtcgc
     5101 cagaggctag caacagcatg ccgcgtcacc tgctttacct gatcgtcttc attgaaggct
     5161 tctgctcact gggtgccgag gtgattgcgc tgcgccgttt ggtgccctat gtcggcagcg
     5221 ccattgtggt gaccgcgccg accatcggcc tgtttctgct ggctttggcg ctgggctacg
     5281 cttctggcgc gcgggtgggt gcccggttca cggccgtcgt tgcacgcaat ttcctgatct
     5341 cgggcctgct cgctggcctg gggctggcgc gcctgacggt ggatggcctt tttgggctgg
     5401 tgcaaccagg ctggctggct tatcttctgt tcatgggcgg cgtgctgtgc ccgctggcct
     5461 ggctgctcgg ccaaaccgtg ccggtgctga ccaacctgat gaagcacgcg cgcgccggcg
     5521 aggccagcgg ctacgcgctg tactggtcca cgctgggctc ctttctgggg tcggtcagcc
     5581 tgtcactggg ggtgatgcag tggctcgggg tgtcggctgc tgtgctgtgc tgtgcggtct
     5641 tgctggcgct gggcagcctg ctgctcgaag gtcaggcgca gccaagctgg caagcctggg
     5701 ccaaggtgct gagcgttggc ctggcagcca gcgccatcaa cctgatgccg gccagcctgg
     5761 tgcaagacac cgcctatgcc gactacgcca tcaaacccct gacgcggccc ggacaagtcg
     5821 aaccacgcgc cttcctgatc aacaattcgg tggcctccat catggacagc agcaagccag
     5881 cgcgctacgc ccgctacatc gagcacctgc ggcacatcat tctggatgaa ctggcgcttg
     5941 aggggcgcga cattctggtg ctgggtgcag gcggcttcaa cctgtcgcat caggaactga
     6001 ccaaccacta cacctatgtt gacattgacg caaaaattcg caccattgcc gaagcagact
     6061 ttctgaaaga gccgattcgc ggtgcctttg tggttgatga tgcgcgccac tttgtcagca
     6121 gcaccacgca acgctttgac gcggtggtgg tggatgcctt cagcagccac acctcgattc
     6181 ccagccacct ggtcacgcgc gagttctggc aggacacccg gcgcaccctg aaaccgcagg
     6241 gggtgttgct gatcaatctg attctggatg gccgactgga gtcagcctat gcccgcaact
     6301 tgctcggcac catcgaaagc gtgtttggcc gctgcgcggt tgaggtgctg cacaagtcgg
     6361 cacccttgag caacgtgatt gtcagttgct accagaccag ccacgcggca ccgacttcac
     6421 cctatacgga tgaacgcaat gctgccgatg tggaccgggc attgtcgcaa taagctgagc
     6481 cattttggca tccagcccat atccaacaaa gatcgatagc tatgaaattg gttgtaagag
     6541 atgccccgcc ccggaccgtc tggacgctgg agcaggccgg catccatccg ctgctggccc
     6601 agctttatgc cgcccgtggg gtgcaagacc cgcaagaact cgacgctgcg ctggcccggc
     6661 tgctggcacc ggccaccatg ctgggcacgg cagcggcggc gcggctgctg gccgatgcca
     6721 tggcacagga caagaaattt tgcatcgtgg cagattacga ctgcgacggg gccaccgcct
     6781 gtgcggtcgc ggtgcgcggc ttgcgactgc tcggcgcgcg ccatgtcgat tacatcgtgc
     6841 cagaccgggt acaggacggc tatggcctga ccccgccgat atcggagcgg gtcaagacca
     6901 gcggggccga cctgctgatc acggtggaca acggcattgc cagttttgac ggtgtgacgc
     6961 gcgcgcgcga tctgggcttg caggtgctgg tgaccgacca ccatttgtgc gccagcttgc
     7021 atggccaggt tgccctgccc gatgcggatg tgatcgttaa cccgaaccag cccggttgcg
     7081 gctttcagag caagtcgatt gccggtgtcg gcgtgatgtt ttatgtgctg ctggcgctgc
     7141 gagccgagtt gcgcttgcgc ggctcgctca atgaacaaac gcagcccagg ctcgacgcct
     7201 tgctgccctt ggtggcactt ggcacggtgg ccgacgtggt acgcctggac gcgaacaacc
     7261 gccgccttgt ggcgcaaggc ctgaaaaggg tccgggccct tgccatgcct gcgggactag
     7321 ctgcactatt tgtagcagct tcgcgtccgg cgacaactgc caccaccttt gactttggct
     7381 ttgccctcgg gccgcgcatc aacgcagcag gtcgcttgtc agacatgacg ctgggcattg
     7441 aatgcctgct gaccgacgat gccgggcgcg cgctggaact ggccaaaacg cttgacggca
     7501 tcaaccgcga acgccgcgag atcgaaagcc acatgcgtga tcaggcattg gccgtggccg
     7561 aagcactttt tgaagacgcc agccatgcgc cgccagccat ctgcgtcttc gatgccgggt
     7621 ttcacgaagg tgtggtgggc attgtggctt cccgcatcaa ggacaaattt caccgcccca
     7681 gctttgtttt tgccagcagc ggtgccgtcg gccacggtca cgaactcaaa ggttccggcc
     7741 gctcgattgc cggttttcac ctgcgcgacg cgcttgacct ggtggccaag cgctaccccg
     7801 gtgtgctgct gcgctttggt ggccacgcca tggcggcagg ttgcaccatt gacgaagcgc
     7861 attttgagac ctttgagcgg ggcttgaatg aagtcgcgca ggaatggctc gacgttgcca
     7921 ccttgacgcg caggctcgac accgatggcc cgctcaagcc cgaataccgc cgcccggacc
     7981 tggtcgatgc cctgcaccac gaggtctggg gccagggctt tgcagcgcca actttcagtg
     8041 aagaactgga ggtggtgtc
//
LOCUS       DAOSLL010000148         8020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8363_length_8020_cov_4.380791, whole genome shotgun sequence.
ACCESSION   DAOSLL010000148 DAOSLL010000000
VERSION     DAOSLL010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8363_length_8020_cov_4.380791"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PLB25_08305"
     CDS             <1..664
                     /locus_tag="PLB25_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE41613.1"
                     /translation="LAYIAIGLFKHLVEGTLRDPASLNKPSTHTLPEGLLIGGIAGSD
                     VHWGLALGVVACVALAIWMRWTASGFSVRVVGGNPRAAQLVGLPASHLVLLACVLGGA
                     CAGLAGAVEVAAVHTNANVSLIAFYGYAGILVSFMARHHPLAIIPVAILFGGFGAAGS
                     LLQRRLGLPDASVLVLQGIAFVLILASEALRDVDWKARLKRLNRAPDTSPAPNPAGVK
                     LS"
     gene            661..1581
                     /locus_tag="PLB25_08310"
     CDS             661..1581
                     /locus_tag="PLB25_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE41614.1"
                     /translation="MTPDQWITLFAGIVGGALRVGTPFLFVSLGECLTEKSGRINLGL
                     EGVLVLSAMASFGGAYLSGSPWVGVLVGALAGGLLAALHGYLCSLARVNDVATGIALM
                     MLGTGLAFYLGKPLIQPQAPQISALSLGMWSDSSAVQAALQVNMLLPLGLLLAALMAW
                     AFKRTRYGLLVRMAGDSANAARALGYSVVGIRVVATTLGGMVAGLGGASLTLFYPGSW
                     NEGISSGQGLIAVALVIFARWSPWRCVGAAALFGGAGAIGPALQSAGIGWGYHLFNTV
                     PYVLTLVILMLTCKPGSVAQGSPGELSATR"
     gene            1594..2298
                     /locus_tag="PLB25_08315"
     CDS             1594..2298
                     /locus_tag="PLB25_08315"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HOE41615.1"
                     /translation="MPHFVQANPYAWPWNGDLRPDNTALIVIDMQTDFCGPGGYVDKM
                     GYDISLTRAPIEPLKTLMAALRATGYPVMHTREGHRPDLSDLPANKRWRSRQIGTDGV
                     GIGDAGPCGRILVRGEPGWDIIPELAPKPDEVVIDKPGKGAFYATDLELVLRTQGIYN
                     LMLAGITTDVCVHTTMRDANDRGFECLLLSDCTAATDYGNHLAALKMVTMQGGVFGAH
                     AASGAVLDALNVQATP"
     gene            2295..3926
                     /locus_tag="PLB25_08320"
     CDS             2295..3926
                     /locus_tag="PLB25_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE41616.1"
                     /translation="MSHTLIPPPRPATGALELETIGLTKRFGSFTAMDQVSMKVAPGT
                     VHALLGENGAGKSTLVKCVAGFQQAEDGNILIDGHEQSLTSPVVARALGIGMVYQHFT
                     LAPGMTVAENLLLAADRVPLLIDWRATRAELSAFLATTPFRLDLDACPSGLAAGEKQK
                     LELLKQLYLKPRLLILDEPTSVLTPQEADEVLGHVREFARSGQCTVIIITHKFREVMA
                     YADTVTVLRRGKAVHHGRVADTTPALLAQAMMGESTGTPQNLESNRSLALIQQSQTAI
                     KIDAKEPLMALQVQGLTAQGDRGTLALHGVNLSVRCGEILGVAGVSGNGQRELVEALV
                     GQRARLAGQVSVMGEAYNATRLENRRLKVRSLPEEPLRNACVGNLSVAENMALRDFDQ
                     APLSAKGLLHFAAWHQRARDWITEYGIKTQGENAPIASLSGGNVQRAVLARELAGEIN
                     ILIAANPVFGLDFAAVEEIHTRLRQACQRGAGVLLISEDLDELLELCDRIVVMSEGRI
                     VFETNAAQADRKELGAHMGGGTHHDTPHPDEITTV"
     gene            3923..4621
                     /locus_tag="PLB25_08325"
     CDS             3923..4621
                     /locus_tag="PLB25_08325"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HOE41617.1"
                     /translation="MSHTSIPRQPMTMTAQPFDYGFDVATTALVIIDMQRDFIEPGGF
                     GESLGNDVSLLSAIVPACQSVLAAWRRAGGAVVHTREAHAADLSDCPPAKRDRGNPSL
                     RIGDVGPMGRILVKGEPGNQIIAELAPLLGEIIIDKPGKGAFYATTLHATLQQLNISH
                     LFFMGVTTEVCVQTSMREANDRGYDSLLLVDCTESYFPAFKAATIAMTHAQGGIVGWT
                     GASEGLLGALAAGT"
     gene            4656..5396
                     /locus_tag="PLB25_08330"
     CDS             4656..5396
                     /locus_tag="PLB25_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013538751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOE41618.1"
                     /translation="MQTVKRRTSAASKPLAEPAERRSRADKVYEQLKQDIAEFKMVPG
                     DRFTENSVTERLDVSRTPVRQALVRLQQEGYVEVLFRSGWRVLPFDFAKFEQLYDLRM
                     VLETTAAYRLCDEGLRSPNPVDRALVDELMALWLVPAVQRSTDTVQVSLWDESFHCTL
                     VVAAGNPEMARVHRDVTDRIRIIRRLDFTKQARIDATYDEHGQILTAIRARRGDQAAM
                     LLRAHIATSQAEVRKITLHQVHMARQSP"
     gene            5589..5873
                     /locus_tag="PLB25_08335"
     CDS             5589..5873
                     /locus_tag="PLB25_08335"
                     /inference="COORDINATES: protein motif:HMM:NF022603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HOE41619.1"
                     /translation="MKTKTKSMPKSTENQSAQQEGDMGIAVYQVCPREQMVAQAAYYL
                     SEQRGFSPGNDLGDWLSAEVYIDSLRREPVTVHGMLHLLRRTNLEPGQRT"
     gene            complement(6153..6482)
                     /locus_tag="PLB25_08340"
     CDS             complement(6153..6482)
                     /locus_tag="PLB25_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOE41620.1"
                     /translation="MTVKPVIARELANSDIDEIIAYYLGESAEQAALGFIDALENAYA
                     HIGHNPGTGSPRYAHELNLPGLRFWPLTRYPHLVFYVERPDHIAAWRVLHGTRDIPFW
                     MIEADDR"
     gene            complement(6479..6730)
                     /locus_tag="PLB25_08345"
     CDS             complement(6479..6730)
                     /locus_tag="PLB25_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HOE41621.1"
                     /translation="MSTMNISLPNTLKTFVDEQVRQGAYGTSSEYLRELIRKDKDRLH
                     LRSLLVAGAASAQAAVADTAYFDGLRERVRKATAVGAHG"
     gene            complement(7098..7922)
                     /locus_tag="PLB25_08350"
     CDS             complement(7098..7922)
                     /locus_tag="PLB25_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41622.1"
                     /translation="MVISKATLTAIHKTGVMAQNLQVALRSEAKKYADRLSKALSDSA
                     DGHVFELLDGPHVNNWKGVGRLSKMVETIEKELMAVMQLATELVADVQKVSEEILAAS
                     APKPVAVAPKKVPAKGKTAVAASNKESNPTKLLSFFTAKLNSDEFTIMTQTEIAKAAG
                     IPMGSITATIKRVAASRAIELNADGTGYKLASKPIQAPAAKPKKLGKATANPVTSAPV
                     VASATPTPAKRAVARKVTTKKAAAKKAIANDAASAIAANPEPAEMTDAPVAVDTVS"
BASE COUNT         1546 a         2282 c         2462 g         1730 t
ORIGIN      
        1 gctggcctac attgccattg gcctgttcaa acatctggtg gaaggcacgc tgcgcgaccc
       61 ggccagcctg aacaagcctt ccacccacac cctgccagag ggcctgctca ttggcggcat
      121 tgccggctca gatgtgcact ggggcctggc actcggggtg gtcgcctgtg ttgctttggc
      181 gatctggatg cgttggaccg cctccgggtt ttcggtgcgg gtggtgggtg gcaacccgcg
      241 tgctgcgcag ctggtagggt tgcccgcctc ccatctggtg ctgctggcct gtgttttggg
      301 cggcgcgtgt gcagggctgg ctggtgcggt ggaggtggcg gcagtgcata ccaacgccaa
      361 tgtctcgctg attgcgtttt atggctatgc tgggattttg gtgtcgttca tggcgcgcca
      421 ccacccgctg gccatcattc cggtggcaat tttgtttggt ggttttggtg ccgcaggcag
      481 tttgctgcag cgccgcctgg ggctgcccga tgcgtcggtg ctggtgttgc agggtatcgc
      541 ctttgtcttg attctggcca gcgaggcgct gcgcgatgtg gactggaagg cccgcctcaa
      601 acgattgaac cgtgcaccgg ataccagccc tgcgcccaac cctgcgggag tcaaactgtc
      661 atgacgccag accaatggat caccctgttt gccggcattg tggggggtgc cctgcgggtc
      721 ggcacgccat ttctgtttgt cagcctgggt gagtgcctga ccgaaaaatc tggccgtatc
      781 aacctgggcc tggaaggcgt gttggtgctg tcggcgatgg cctcgtttgg aggtgcctac
      841 ctgagtggct cgccctgggt gggggtgctg gtcggcgctt tggcgggtgg cttgctggcc
      901 gccttgcatg gttatttgtg ttcccttgcc cgggttaacg atgtggccac cggtattgcc
      961 ctgatgatgt tgggcaccgg gctggcgttc tacctgggca aacccctgat tcagccgcag
     1021 gcaccgcaaa tttcagccct atcgctaggc atgtggagcg actcgtcggc ggtgcaagct
     1081 gccttgcagg tcaatatgct gctgccactg ggcctgctgc tggcggcgct gatggcctgg
     1141 gctttcaagc gcacccgtta tgggctgctg gtgcgcatgg cgggtgactc ggcgaatgcg
     1201 gcgcgcgcgt tgggttattc ggtggtcggc attcgtgtgg tggcgaccac gctgggtggc
     1261 atggtggcgg gcttgggtgg cgcgtcgctg acgctttttt acccgggcag ctggaacgaa
     1321 ggtatctcca gcggccaagg cctgattgcg gtggcgctgg tgatttttgc acgctggagc
     1381 ccctggcgct gtgtaggtgc ggcggccctg tttggcggag ctggtgcaat cggcccggcg
     1441 ctgcagtcgg ccggtatcgg ctgggggtac cacctgttca acaccgtgcc ttatgtgctg
     1501 acgctggtca ttttgatgct gacctgcaaa cccggcagcg tggcgcaagg cagccccggc
     1561 gagttgtcgg ccacccgcta actggagatt tgtatgccgc atttcgttca agccaacccg
     1621 tatgcctggc cctggaacgg cgatttgcgg cccgacaaca ccgcgttgat cgtgatcgac
     1681 atgcaaaccg acttctgcgg cccgggtggt tatgtggaca aaatggggta tgacatcagc
     1741 ctgacccgcg ccccgattga acccctgaaa accctcatgg cagccttgcg tgcaacaggt
     1801 tacccggtga tgcacacccg cgaagggcat cggcccgatt tgtcggactt gcccgccaac
     1861 aagcgctggc gttcacgcca gatcggcacc gatggcgtgg gcattggtga cgccggcccc
     1921 tgcgggcgta ttctggtgcg tggtgagccg ggctgggaca tcatccccga gctggcacca
     1981 aagccggatg aggtagtgat tgacaaaccc ggcaaaggcg cgttttacgc caccgacctg
     2041 gagttggtgc tgcgcaccca gggcatctac aacctgatgc tggccggcat caccacagat
     2101 gtgtgtgtgc acactacgat gcgcgatgcc aatgaccgcg gttttgagtg cctgctgctg
     2161 tcggactgca cagccgccac cgactacggt aaccacctgg cagctttgaa gatggtcacc
     2221 atgcaaggtg gtgtgttcgg cgcccacgcc gcctctggcg cggtgctgga cgccctgaat
     2281 gtgcaggcca cgccatgagc cacacgctga ttccgccccc gcggcccgcc actggcgcac
     2341 tggagttgga gacgataggc ttgaccaaac ggtttggcag cttcacggcg atggaccagg
     2401 tcagcatgaa ggtggcaccc ggcacggtgc acgcgctgct gggcgaaaac ggcgccggta
     2461 aaagcactct ggtcaagtgt gtggccggtt ttcagcaagc cgaagacggc aacatcctga
     2521 ttgatggcca cgagcaaagc ctgacaagcc cggtggtggc gcgggcgctg ggtattggca
     2581 tggtgtacca gcatttcacc ctggccccgg gtatgacggt ggccgaaaac ctgctgctgg
     2641 cggcggaccg ggtgccactg ttgatcgact ggcgggccac acgcgccgaa ctcagtgcct
     2701 ttttggcgac cacgccgttc cggctcgacc tggatgcctg cccgtcaggt ctggcggctg
     2761 gcgaaaaaca gaagcttgaa ctgctcaagc agttgtacct gaaaccccgg ttgctgatcc
     2821 tagatgagcc aacctcggtg ctgacgcccc aagaggccga tgaggtgttg ggtcatgtgc
     2881 gcgaatttgc ccgcagcggg cagtgcacgg tgatcatcat cacccacaag ttccgcgaag
     2941 tcatggccta tgccgacacg gtgaccgtgt tgcgccgggg caaggcggtg caccatggcc
     3001 gggtggcaga caccaccccg gcgttgctgg cccaagccat gatgggagaa tccactggta
     3061 ctcctcaaaa tttggagtca aatcggtctc tagcgcttat tcaacaatcc cagacagcta
     3121 taaaaataga tgcaaaggag cctctgatgg cgctgcaggt gcagggcttg acagcccagg
     3181 gcgaccgtgg cacgctggcc ttgcatggtg tgaacctgtc tgtgcgctgt ggcgaaatcc
     3241 tcggcgtggc aggtgtatcg ggcaatggcc agcgcgagct ggtcgaggcc ctggttggtc
     3301 aacgcgcgcg cctggccgga caggtcagcg tcatgggcga agcctacaac gccacccgcc
     3361 tggagaatcg ccgcctgaag gtgcgcagcc tgcccgaaga acccttgcgc aacgcctgtg
     3421 tgggcaacct gagtgtggcc gagaacatgg ccctgcgcga ctttgaccag gcaccgctct
     3481 ccgccaaggg gttgttgcac tttgccgcct ggcaccaacg ggcgcgcgac tggataaccg
     3541 aatatggcat caaaacccag ggcgaaaacg caccaattgc cagcctgtct ggcggcaatg
     3601 tgcaacgcgc tgtgctggcg cgcgaactgg cgggcgagat aaacatcctg attgccgcca
     3661 acccggtgtt tggcctggac tttgccgccg tggaagaaat tcacacccgg ctgcgtcagg
     3721 cctgccagcg cggggccggt gtgttgctca tcagcgagga ccttgacgaa ttgctggagc
     3781 tgtgtgaccg tatcgtggtc atgagtgaag gccgcatcgt gtttgagacc aacgccgcgc
     3841 aggccgaccg caaagagctg ggcgcccaca tgggcggcgg cacccaccac gacacccctc
     3901 atccagacga aatcaccacc gtatgagcca cacttccatc ccccgccagc ccatgaccat
     3961 gacggcgcaa ccttttgact atggttttga cgtcgccaca acagcgctgg tcatcatcga
     4021 catgcagcgc gatttcatcg agccaggcgg ttttggtgaa tcgctgggca acgatgtgtc
     4081 tttgctgtcg gcgatcgtgc cggcctgcca gagtgtactg gcggcgtggc ggcgcgcagg
     4141 gggtgcggtg gtgcacaccc gcgaggccca cgcagctgac ctgtccgact gcccaccggc
     4201 caaacgggat cggggtaacc ccagtttgcg cattggcgat gtcggtccaa tggggcgtat
     4261 tttggtgaag ggcgagccgg gcaaccagat cattgccgaa ctcgcccctt tgcttggcga
     4321 aatcatcatc gacaaacccg gcaaaggcgc cttttacgcc accactttgc atgccacgct
     4381 gcagcagctg aacatcagcc atctgttttt tatgggcgtc accaccgagg tgtgcgtgca
     4441 aaccagcatg cgcgaagcca acgaccgggg atatgacagc ctgctgctgg tggactgcac
     4501 cgagagttat ttcccagcgt tcaaggccgc caccatcgcc atgacccacg cccagggtgg
     4561 tattgtgggc tggacggggg ccagcgaggg cttgctgggt gccctggccg ctggcaccta
     4621 ggtccagcgt tggaaggctg gtaaagtcag cctcaatgca aaccgtgaaa cgtagaacca
     4681 gtgccgcctc caagcccttg gccgagccag ccgaacgccg ctcccgcgct gataaagtct
     4741 acgaacagct caaacaagac attgccgaat tcaaaatggt gccgggcgac cgctttaccg
     4801 aaaactcggt gaccgaacgg cttgatgtct cgcgcacacc ggtgcgccag gctctggtca
     4861 ggctgcagca agagggttat gtggaggtgc tatttcgcag tggctggcgt gttttgccat
     4921 ttgattttgc caagtttgaa caactttacg acttgcgcat ggtgctggaa accactgccg
     4981 cctaccggtt gtgcgacgaa gggctgcgca gcccgaaccc ggttgaccgc gcgctggtcg
     5041 atgaattgat ggccctgtgg ctggtgccag cggtgcaaag aagcacagac accgtgcagg
     5101 tcagtctgtg ggatgagtcc tttcactgca cgctggtggt ggcagcgggt aaccccgaaa
     5161 tggcgagagt gcatcgcgac gtgaccgacc gcatccgcat cattcgccgc ctggatttca
     5221 ccaaacaggc gcgcattgat gccacctacg acgaacacgg ccagattctt accgccattc
     5281 gcgccaggcg gggtgatcag gcggccatgc ttttacgcgc gcacattgcg accagccaag
     5341 ctgaggtgcg aaaaatcacg ttgcatcagg tacacatggc acgacagagc ccatagtgct
     5401 ttctggggca aaggttgatg accgctaccc caatgttgca ctgctgcaaa agggagttac
     5461 ccccaccaat gccagtaata aacccctgca acgggttaca aagatgcttg tgtgggtatt
     5521 cccattctgt tggactccgc ctacgatctc ctgatgccat atcaatcaat tccaatcagg
     5581 agagacaagt gaagaccaaa accaaatcaa tgcccaagtc gactgaaaat caatcagcgc
     5641 agcaagaagg cgatatgggt attgctgtat atcaagtctg tccacgcgaa cagatggttg
     5701 cccaagcagc gtattacttg tcagaacagc gtgggttttc gcccggaaac gatttgggcg
     5761 actggttgag cgccgaagtt tacattgata gcttgaggag ggaaccggtc acagttcacg
     5821 gcatgcttca cctattgcgg cgcactaacc ttgagcctgg gcagcggact tgatgacggg
     5881 ctgaaggtcc tggcaaaact agacaaaaaa ggtcaatttg gctaaatacg cccatcccct
     5941 caaaaaggta aaacgagcga actttcccgc ttagccaaat gttccaatca cccggaattc
     6001 ggatatcaga ggggatatgc cggcgcagag cttcctccag cccgatgcga tcaatgaagt
     6061 gattaaccta cgtgcagttg aaacgtagac ccttcaatca gatgctggga gtgccaaagc
     6121 ccgcagtaac cccttttggc tcggcaaaag aatcagcggt catcggcctc gatcatccaa
     6181 aaaggaatgt cccgcgtgcc gtgcagcacg cgccaagcag cgatgtggtc gggccgttca
     6241 acgtaaaaga caaggtgcgg atagcgagtc agtggccaaa accgcagccc gggcaggttc
     6301 aattcgtgcg catagcgtgg tgacccagtc cctggattgt gaccaatatg tgcgtaggcg
     6361 ttttcaagtg catcgatgaa tcccagggcc gcttgctcag cggactcgcc cagatagtaa
     6421 gcaatgattt cgtcaatgtc agaattggcc agctcgcgag caatgactgg tttgactgtc
     6481 aaccgtgcgc acccactgcg gtggctttgc gaacccgctc gcgcaggcca tcaaagtacg
     6541 ctgtatcggc cacagcggct tgcgcagagg cagcaccagc caccagcaga ctgcgcaagt
     6601 gcagtcggtc tttgtctttt cggatcagtt cgcgcaggta ttcactgctg gtgccatacg
     6661 cgccttggcg aacttgctcg tcaacaaagg tcttcagggt gttgggcaaa gagatgttca
     6721 tggtgctcat gctcacagtt tagagttctt ggcaaaattt ggcaaggttg atgcgtgttt
     6781 tccgaccgcg atcacttgag acggagggcc ttagtaggtg gccacctgca gtggactgtg
     6841 agtacgcgta tagcgcgcga tgaatgtctg cgcgtcaacc ttgcggtact cggtattgat
     6901 ggtgttcaag gactttgtca ttccaaaaag ggcggcccgc acatcctaca aggttgcaac
     6961 gggccgccaa ggcttttagc aagcgataaa aaaacccgct gaggcgacac gcgcacagcg
     7021 ggttcagagc tgggtgatca agctaaattc agtcgttcat gcaggctttc gactttccgg
     7081 ggtgcgatcc gatccgatca agatacagta tcaactgcca ctggcgcatc cgtcatttct
     7141 gcgggctcag ggtttgcggc gattgcggat gctgcatcgt ttgcaatggc cttcttggca
     7201 gctgctttct tggtcgttac cttccttgct acagctcttt tggctggcgt cggtgtggct
     7261 gatgccacaa ccggtgcaga cgttacagga tttgccgtcg ccttgccgag cttctttggt
     7321 ttggcagccg gtgcttggat tggcttgctc gccaatttgt aacccgtgcc atcggcgtta
     7381 agttcgattg cgcggctagc cgccacgcgt ttgatggtgg cggtgatcga gcccatcggg
     7441 atgcctgcgg ctttggctat ctcggtttgc gtcatgatcg taaattcatc gctgttgagt
     7501 ttggctgtaa agaagctcag cagttttgtg gggttcgatt ctttgttgct tgccgcaact
     7561 gcggttttgc ccttggcagg caccttcttc ggtgccactg caacaggttt tggcgcgctg
     7621 gctgccagaa tttcttccga caccttttga acatcggcga cgagttcagt ggcgagttgc
     7681 atcaccgcca tcaactcctt ctcgatggtt tcgaccatct tggagagtcg acccactccc
     7741 ttccagttgt tgacatgagg gccatcgagt aactcaaaga catgaccatc ggcgctgtca
     7801 gagagtgctt tgctcagacg atccgcgtat tttttggcct ctgagcgcag tgccacctgc
     7861 aggttctgcg ccatcacgcc agtcttatga attgccgtca aggttgcttt tgaaatgacc
     7921 atgctgcttg tctttctgat agttcgattt cgccgcacta acgaaaaatc ttcacccttg
     7981 cggcgggcgt gaattatcgt tcaatagtat accgatgcat
//
LOCUS       DAOSLL010000149         7929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8462_length_7929_cov_4.353950, whole genome shotgun sequence.
ACCESSION   DAOSLL010000149 DAOSLL010000000
VERSION     DAOSLL010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7929
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8462_length_7929_cov_4.353950"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            34..>1232
                     /locus_tag="PLB25_08355"
     CDS             34..>1232
                     /locus_tag="PLB25_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE41623.1"
                     /translation="MPFFADNAAETTKMASELQNAIEPLMTSSEEKELFTRISEVRKA
                     YLASRSQVTKLKSEGRANEANDTLEKAFIPLSNSYLKLVGELLEVQHKTLDQKASRIS
                     DIEQTSRTNLLALTLLTIAVGAAFAWLLTIGITGPLSDAAVLLRRVSVGDLGNTISPQ
                     GRDETAQLLQALKDMQTRLAKVVSQVRQGSESVAIASSEIARGNHDLAARTERQASAL
                     EETVVSMEELGATVRKNADSAMQASQLAQSASTVAVKGGAVVAQVVDTVRGINDASKK
                     ISDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLASRSADAAKEIK
                     TLINTSVERVEQGTALVDQAGVTMTEVVNSIRRVTDIMGEISSASSAQTMGVSQVGES
                     VALMDQAT"
     assembly_gap    1233..1332
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1333..1565
                     /locus_tag="PLB25_08360"
     CDS             <1333..1565
                     /locus_tag="PLB25_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE41624.1"
                     /translation="TMGVSQVGESVALMDQATQQNAALVEEMAAAASSLKTQAQELVD
                     TVAVFKLDANDSHHGLTSALALQSPPDFDGVR"
     gene            1849..2034
                     /locus_tag="PLB25_08365"
     CDS             1849..2034
                     /locus_tag="PLB25_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41625.1"
                     /translation="MAKPRLLRQRRRLLAVLLGMARTNVRCLEVVNPALSGLLLQIAC
                     GIGRIRVLRRSGKTASR"
     gene            2085..3101
                     /locus_tag="PLB25_08370"
     CDS             2085..3101
                     /locus_tag="PLB25_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012406705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE41626.1"
                     /translation="MKFSRAAVRFAFAILLTYLGLLAVTFFIGRVIPVDPVLAVVGDH
                     APEHVVARVREEMGLNKPVYLQFGIYVSKVLTGDFGVSVLTSNPVLQDIRRTFPATFE
                     LATLGILIGAGLGVPLGVWAAVRRGGMADQVVRVMGLIGYSVPIFWLGLMALVLFYAR
                     LGWVGGPGRIDVTYEYLFTPVTGILLLDTARAGQWAAFGNVLSHLILPASLLGYFSLA
                     YISRMTRSFMLNELSQEYVVAARAKGLSEARIIWRHALRNAMVPLVTVITLSYAGLLE
                     GSVLTETVFAWPGLGLYITNSLQNADMNAVLGGTIVVGSIFIGLNLLSDLLYRTLDPR
                     THLR"
     gene            3291..4226
                     /locus_tag="PLB25_08375"
     CDS             3291..4226
                     /locus_tag="PLB25_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE41627.1"
                     /translation="MNAQDVVTVAQAGGLHAWLMSDRPVSRRQAALGRAYGAWRTFGR
                     NRLAMLGLVIVLALVLVALFANQLAPYSPTVGDLRTTRLLPPSANFWLGTDDQGRDIL
                     SRLIHGSRITLFVVFLVAVLAAPIGLLVGTVAGYAGGLVDATLMRITDIFLAFPRLIL
                     ALAFVAALGPGIENAVIAIAITSWPAYARLARAETLTIRHADFIAAVQLIGASPWRIV
                     FRHIMPLCVSSVIVRVTLDMAGIILTAAGLGFLGLGAQPPSPEWGAMIANGRQYVLDQ
                     WWVAAAPGGAIFLISLAFNLLGDGLRDALDPKGVQ"
     gene            4223..4354
                     /locus_tag="PLB25_08380"
     CDS             4223..4354
                     /locus_tag="PLB25_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41628.1"
                     /translation="MIFALGAAAQVVGIAPTVTDLRRIGIRPDLCCVVPAIEAGVAG"
     gene            4356..5213
                     /locus_tag="PLB25_08385"
     CDS             4356..5213
                     /locus_tag="PLB25_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE41629.1"
                     /translation="MSQSELSMEPMLVVDDLRVAFPSRDGGWVEAVRGVSFVLGRERL
                     GIVGESGSGKSQTGRAIMGLTAPDARVSARRLSFNGVDLLYCTPAQRRALRGGRIAMV
                     LQDPKFSLNPVMTIGQQIVETLQAHSRVSAAEARLQALAALASVQIDDPKRVFKLYPH
                     QVSGGMGQRAMIAMMLIARPDLLIADEPTSALDVTVQLEVLEILDALVLQRGMGLIFI
                     SHDLRLVSGFCDRILVMYAGRVVEELPAGGLTNAQHPYTQGLLNCLPKLGVDQHPLPT
                     LKRQPEWAQ"
     gene            5210..6031
                     /locus_tag="PLB25_08390"
     CDS             5210..6031
                     /locus_tag="PLB25_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE41630.1"
                     /translation="MNQASAPASVTGTASQYKSVGLEVQNLRVEYGDFVAVADTSFAV
                     QAGDSFGIVGESGSGKSTVLRAICGLAASTGRVQLLGPLAQTLPAPGSKPFRRLVQMV
                     FQDPYGSLHPRHTVDKILAEPLAIHGIKNEQARIEQALDEVGLGQGFRFRYPHQLSGG
                     QRQRIAVARALILEPQILLLDEPTSALDASVQAEVLNLLEGLRIRRGLTFIMVSHDLA
                     VVTHLCQRLMVMQRGQTVELLSSGDLTANRTKDPYTQKLMQASGGFRRTLAVRQK"
     gene            complement(6446..6562)
                     /locus_tag="PLB25_08395"
     CDS             complement(6446..6562)
                     /locus_tag="PLB25_08395"
                     /inference="COORDINATES: protein motif:HMM:NF021255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HOE41631.1"
                     /translation="MSTRNKAFDNLTPVDIVKEWGFAGLLMVRAYLDRARGV"
     gene            complement(7241..>7929)
                     /locus_tag="PLB25_08400"
     CDS             complement(7241..>7929)
                     /locus_tag="PLB25_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE41632.1"
                     /translation="IIGTIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLA
                     GRSAEAAKEIKTLINDSVNRVEQGTKLVDQAGSTMQEVVNSIHRVTDIVGEISSASQE
                     QNQGVAQVGEAVTQMDQVTQQNAELVEEMAAAASSLKTQAQDLVQTVAVFKLGDGDPA
                     ASSLPTATVRGHPPKTTQSKGVDRRSAGVSKVAAVRAPSVPPPTLTNSVKPAGTTTSS
                     TNGADDWETF"
BASE COUNT         1428 a         2221 c         2502 g         1678 t
ORIGIN      
        1 tcggcgattg caaaaagcac tgaccctgcc ctggtgcctt tttttgctga caacgccgcc
       61 gaaaccacca agatggcctc agagctgcaa aatgccatcg aacctttgat gaccagtagt
      121 gaagagaagg aacttttcac caggatttcg gaggtccgta aagcctatct ggcaagtcga
      181 agccaggtta caaagctcaa atcggagggc cgggccaatg aggcgaatga cactttggaa
      241 aaggcattta ttccgctttc gaacagttac ctgaagctgg tgggtgaact gcttgaagtt
      301 caacacaaaa cccttgatca aaaggcgagc cgcatttccg acattgagca gaccagccgc
      361 accaatctgc ttgcgctcac gttactgaca attgcggtgg gggcagcgtt tgcgtggctg
      421 ctgaccatcg gaattaccgg cccgctgtca gacgcggcgg tgctgcttag gcgggtctca
      481 gtgggtgatc tgggcaacac gatttcaccg caggggcgcg atgaaaccgc acaactcttg
      541 caggcgctca aagacatgca aaccagactg gccaaggtgg tcagccaggt gcgccagggc
      601 tccgaaagtg tggcgattgc ctcatccgag atcgcccggg gcaatcacga cctcgcggcc
      661 cgcaccgaac ggcaggccag cgctttggaa gagacagtcg tctcgatgga agagctcggt
      721 gccacggtaa ggaaaaacgc cgacagcgcc atgcaggcca gtcaactcgc gcagagcgcc
      781 tctaccgtgg cggtcaaggg tggtgccgtt gtggcacagg ttgtcgatac cgtgagaggc
      841 atcaacgatg cctccaaaaa gatatcggac atcatcagcg tcatcgacgg cattgccttc
      901 cagaccaaca tcctggccct caatgcggcg gtggaagcgg cccgggccgg cgagcagggc
      961 cgcggttttg cggtggtggc atccgaagtc cggtccctgg ccagtcgaag cgccgatgcc
     1021 gcgaaagaaa tcaagacctt gatcaacacc agcgtggagc gggttgagca gggcacagcc
     1081 ctggtcgatc aggcaggcgt gaccatgacc gaggtggtca acagtatccg ccgcgtcacc
     1141 gacatcatgg gcgaaatcag ttccgccagc tccgcgcaga ccatgggtgt gtcgcaagtc
     1201 ggtgagtccg tggcgctgat ggatcaggcc acnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnagaccatg ggtgtgtcgc aagtcggtga gtccgtggcg ctgatggatc
     1381 aggccacgca gcagaatgcg gctctggttg aagaaatggc ggctgccgct tccagcctca
     1441 aaactcaggc acaggagctg gtggacacgg tggcggtgtt caaacttgat gccaatgaca
     1501 gtcatcatgg tttgaccagc gccttggcgc tgcagtctcc accggatttc gatggggtac
     1561 gctagcaccg tgagtgatgt gtcagtgagg tgtcagtgat gcgtcggtga agcgagcctg
     1621 accccgtcaa tccgtccggc actgcttgcc cagtcggagc aagcagtgcc gatcaagcgc
     1681 agcaggaatc tgccaaagtt gcttcataaa gcatagctgg tagcgcaatg tacacggggg
     1741 ctggaggcca ttttgaccct gatgtcgggc tgaatgtgct gaccattgcc gcagcagcag
     1801 cgctccaaca atccagagcc agagccagtg ccagtgccag tgcagcggat ggccaaaccc
     1861 cggctgcttc gccagcgccg ccgcctgctg gccgtcctct tgggcatggc ccgcaccaac
     1921 gtccgctgcc ttgaggtggt caacccggca ttgagcggct tgctgctgca gatagcctgc
     1981 ggcatcggcc gaattcgggt gttgcggcgg tccggcaaga cggcaagccg ataggccatg
     2041 ctgacggcct tgcactgata atggtcgccc actatgttga tcccatgaag ttctcccgcg
     2101 ctgccgtccg ctttgccttc gccatcttgc tgacctatct gggtctgctg gccgtgacct
     2161 ttttcattgg ccgcgtcatt ccggtggacc cggtgctggc ggttgtgggt gaccacgcac
     2221 ccgagcatgt ggtggcccgc gtgcgcgagg aaatggggtt gaacaagccg gtttacctgc
     2281 agtttggcat ttacgtgtca aaagtgctga cgggtgattt cggcgtgtcg gttctgacat
     2341 cgaacccggt gctgcaagac atccggcgca ctttcccggc cacttttgag ttggccacgc
     2401 tggggatctt gatcggtgcc gggcttggcg tgccgctggg agtctgggct gcggtgcggc
     2461 gcggcggtat ggcagatcag gtggtgcggg tgatgggcct gatagggtat tcggtgccga
     2521 ttttctggct tgggctgatg gcgctggtgc tgttttacgc acgcttgggc tgggttggcg
     2581 ggccggggcg aatcgatgtg acctatgagt acctgttcac accggtcacc ggcatcttgc
     2641 tgcttgacac cgcccgtgcc gggcaatggg ctgcctttgg caatgtgctg tcgcatctga
     2701 tcttgccagc ctcgctgctt ggctattttt cactggccta catcagccgc atgacgcgtt
     2761 cattcatgct caatgagttg tcgcaagagt atgtggtcgc ggcccgtgcc aaaggcttgt
     2821 ccgaggcgcg catcatctgg cggcacgcgc tgcgcaatgc gatggtgccg ctggtcaccg
     2881 tcatcacgct gtcgtatgca ggtttgcttg aaggctcggt gctgaccgag acggtatttg
     2941 cctggcccgg gctgggcctg tatatcacca attcgctgca aaacgccgac atgaacgcgg
     3001 tgctgggcgg caccattgtg gtcggctcga tcttcatcgg tctgaatttg ctgtccgacc
     3061 tgctgtaccg cacgctcgac ccgaggacgc acctgagatg agcacggtgc cgacgacatc
     3121 aagcatgcaa atgatcaccc ggctggtctt caatcaccag tgtgccgctt gaggttatca
     3181 aatatgatag ctatcagcgc aattacagca agggatggag cctgttttga tgatcaaaag
     3241 accatcagcc tggaaggctg tgcagccagc ttggcggggg tgctgccgcg atgaacgccc
     3301 aagatgttgt gaccgttgcc caggccgggg gcctgcatgc ctggctgatg tcggatcgac
     3361 cagtttcgcg ccgtcaggcc gcgctggggc gggcctatgg cgcgtggcgt acctttggcc
     3421 gcaaccggct tgccatgctg gggctggtta tcgtgctggc gctggtgctg gttgcgctgt
     3481 ttgccaacca gttggcacct tactcgccca cagtgggtga cttgcgcacc acgcgcctgc
     3541 tgccaccttc ggcaaatttc tggctgggca ccgacgatca gggccgcgat attttgtcgc
     3601 gcctgattca tgggtcgcgc atcacgctgt ttgtggtgtt tttggtggcg gtgctggcgg
     3661 ccccaattgg cttgctggtc ggcaccgtgg cgggatatgc tggtggcctg gtggatgcga
     3721 cgctgatgcg catcaccgat atttttctgg cctttccacg gctgattctg gcgctggcct
     3781 ttgtcgctgc gctcgggccg ggaatcgaga acgcggtgat cgcgatagcg attacttcct
     3841 ggccagccta cgcgcggctg gcgcgggccg aaaccctgac catccggcac gccgatttca
     3901 tcgctgccgt gcagttgatt ggtgcgtcgc cctggcgcat tgtgttcaga cacatcatgc
     3961 cgctgtgtgt gtcgtcggtg attgtgcgcg tcacgctgga catggcgggc atcatcctga
     4021 cggctgccgg gctgggcttt ctggggcttg gtgcgcaacc gcccagtccg gaatggggcg
     4081 cgatgattgc caatggccga cagtatgtgc tggaccagtg gtgggtggca gcagcgccgg
     4141 gcggggcgat ttttctgatc agcctggcgt tcaacctgct gggcgacggt ctgcgcgatg
     4201 cacttgaccc gaaaggcgtg caatgatctt tgcgcttggg gctgctgccc aggttgttgg
     4261 catcgctccc accgtgacag acctgcgcag aatcgggatt cgacccgatt tgtgctgcgt
     4321 cgtgcccgcc atcgaggccg gggttgcggg ctgaaatgag ccaaagcgag ctttccatgg
     4381 agccgatgct ggtggtggac gacttgcgcg tggcgtttcc gagccgtgac ggcggctggg
     4441 ttgaggcagt gcgcggtgtg tcatttgtgc tagggcgcga acgcctgggc atcgtcgggg
     4501 aatctggctc gggcaagtca caaaccggtc gggcgatcat gggactgacc gcccctgatg
     4561 cgcgcgtcag cgccaggcgg ctgtctttca atggcgtgga tttgttgtac tgcacgcccg
     4621 cacagcggcg cgcgctgcgc ggtggccgta tcgccatggt gctgcaagac cccaagtttt
     4681 cgctcaaccc ggtgatgacg attggccagc agatcgtcga aaccttgcaa gcgcacagcc
     4741 gtgtatcggc cgctgaagcc cggttgcaag cattggcggc gctggcatcg gtgcaaattg
     4801 acgacccaaa gcgggtcttc aagctctatc cacaccaggt ttcgggcggt atgggccagc
     4861 gcgccatgat tgccatgatg ctgatcgcca ggccagacct gctgattgcc gatgaaccca
     4921 cgtcggcgct ggatgtcaca gtgcaactgg aggtgctgga aatcctcgat gcgctggtgc
     4981 tgcagcgcgg catggggctg attttcatct cgcacgactt gcgtctggtg tcgggctttt
     5041 gcgaccgcat cctggtcatg tacgctggcc gggtagttga agaattgcca gccggtggct
     5101 tgaccaacgc ccagcacccc tacacccaag gcctgctcaa ctgcctgccc aagctcggcg
     5161 ttgaccagca tccgctgcca acactgaagc gtcagccgga gtgggcgcaa tgaaccaggc
     5221 aagtgcgcca gccagcgtca ctggcaccgc aagccagtac aagtcggttg ggctggaagt
     5281 gcagaacttg cgtgtcgaat acggcgactt tgtggcggtg gcagatacct cgtttgccgt
     5341 gcaagcgggc gacagctttg gcatcgtggg cgaatctggc tcgggtaaat caaccgtgct
     5401 gcgtgccatt tgtgggctgg cagccagcac cgggcgggtg cagttgctgg ggccgctcgc
     5461 gcagaccctg ccagcgccgg gcagcaagcc gtttcgccgg ctggtgcaaa tggtgtttca
     5521 ggacccttac ggttcactgc atccgcgcca cacggtggac aagattctgg ccgagccgct
     5581 ggccatccat ggcatcaaga acgagcaggc acgcattgaa caggcgctcg atgaggtcgg
     5641 cttgggccag gggttcaggt ttcgataccc gcaccagttg tcgggcggcc aacgccagcg
     5701 cattgcggtg gcgcgcgcgc tgattctgga gccgcagatt ttgctgcttg acgaaccgac
     5761 ctcggcgctg gatgcttcgg tgcaggcaga ggtgctgaac ctgctggagg gcctgcgtat
     5821 acgccggggt ctgaccttca tcatggtcag tcatgatctg gcggtagtga cgcacctgtg
     5881 tcagcgcctg atggtgatgc agcgcggcca gacggtggag ctgctcagtt ctggcgacct
     5941 gacggccaac cggacaaaag acccctacac gcagaaattg atgcaagcct cgggtggttt
     6001 tcggcgcact ttagctgtca ggcaaaaatg acttgaacct ctggccgcac tggcaggtgc
     6061 catgttttgg tgccacccgc ttgtgtagcc agttgcaccg cgcgactcgc agtttggtac
     6121 tgggttgcat ccacattggg catcaaaacg cagcagtgtc gattgccatc caggcaactc
     6181 cgggctgttt ctcaataacg atgcaatggc cttcaagctg atatgacaat tggcaatggt
     6241 gtcgtgggcc agacagaaca tcggggttca ggacgacaca gtcatgaccc gccgcatggt
     6301 gcacggatgt cgtcaccagt cctggatgcc cttcgcggtg aagtcttgcg cctgccgact
     6361 gggcggtgcg gtcgtcggtc ttgtgcatca agcctgaatg tgtggcggtc atccgtcagt
     6421 gtgaggtgag agaacaactg atccatcaaa cgccacgcgc ccgatccaga taggcgcgca
     6481 ccatcaacaa gccagcaaaa ccccactcct tcacgatgtc tacgggggtc aggttgtcaa
     6541 atgccttgtt gcgggtggac attcagcggt atgccaaatc acggttttgc gggaacagca
     6601 gccgtagttt cttgtgaata cccggtagat ggccaagcgc agttggagcg gtgccttcaa
     6661 tttctcgcac cactttcaaa gccaggcttt ttcacccacg atgatggtcg ctgagtacac
     6721 gcagagaatg tcgcaaccca caaattctca aaaaaaatga tattgctgtg attcaaactg
     6781 agcaccagag ccactgtttt ttgtttgcgc aacaggccag ttgcggtagt ggcactttgc
     6841 gacttgcctt gtccagaccg gattttgaag atggtttatc cccggcacgc gctccaacgc
     6901 cgagggatga ctgtggtgat gcgcggatgg atgacgaggc gcaaatcgag ccggaatggg
     6961 caaccgacgg ggatttggcg gcaccatcgg cgccggacta tgaggtcgat cagcgcatca
     7021 gttggtgaaa ggccgaggtg aacattgcga ctgctgcagc aatggtgtgt ctatgtggtt
     7081 gactcaaaat cgaacttgcg gcagcgggcg caccaacggg catctgcaac ggatcaaact
     7141 gcacccgtca aaggtgtctg ttcgcaccaa gccggtgcaa tgctctgcct catgcggttg
     7201 aaatctgtcc gtttttgact ccaggcgctg agtggctgga ctaaaacgtt tcccaatcgt
     7261 ctgcgccgtt ggtcgatgaa gtggtggtac ccgccggttt gaccgagttg gttagcgtag
     7321 gcggtgggac gctgggcgcg cgaactgctg cgaccttgga tacacctgca gaacgtcggt
     7381 caacgccctt ggactgagta gttttcggtg ggtgcccgcg caccgtagcg gtgggcaggc
     7441 tgcttgcggc gggatcaccg tcaccgagct tgaacaccgc taccgtctgt accaggtctt
     7501 gcgcttgcgt tttcaggctg cttgccgctg cggccatttc ttcgaccagc tcggcgtttt
     7561 gctgcgtgac ctggtccatt tgcgtgaccg cctcgcccac ttgcgccaca ccctggtttt
     7621 gctcctggct ggcgctgctg atctcgccta cgatgtcggt cacgcggtgg atgctgttga
     7681 ccacctcttg catcgtgctg ccggcctgat cgaccagctt ggtgccttgt tcgacccggt
     7741 tgacgctgtc gttgatcagg gtcttgatct ctttggctgc ttcagcgctg cgcccggcca
     7801 gagagcgcac ttcagaggcc accacggcaa agccacggcc ctgctcgccg gcgcgggcag
     7861 cttccaccgc ggcattcagc gccaggatgt tggtctggaa ggcgatgccg tcgatggtgc
     7921 cgatgatgt
//
LOCUS       DAOSLL010000150         7858 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8557_length_7858_cov_4.567859, whole genome shotgun sequence.
ACCESSION   DAOSLL010000150 DAOSLL010000000
VERSION     DAOSLL010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7858
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8557_length_7858_cov_4.567859"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(133..855)
                     /locus_tag="PLB25_08405"
     CDS             complement(133..855)
                     /locus_tag="PLB25_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HOE41633.1"
                     /translation="MTYCVGVKLDEGLIFASDSRTHAGVDNYARFCKMTVFERTGDRV
                     LVLLSSGGLAATQTVINILRQRAAAGPPHIWSVGSMFDVANLVADAMRDLERRDGKYL
                     QSGGLKFNASFILGGQIAGEDMRLFRIYAEGNFIEAGTDTPFLQTGEAKYGKPILDLV
                     VTSSATLEDASKCVLVSFDTTMLSNLSVGMPIDLLCYAQDSLKVTMKRRFDRGDADFE
                     ALMQLWLAGTRKVFRELPPLQW"
     gene            complement(876..1688)
                     /locus_tag="PLB25_08410"
     CDS             complement(876..1688)
                     /locus_tag="PLB25_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOE41634.1"
                     /translation="MQIRVGFEMVYECPDPTPMILALNIHHSRAPDLVRPDLLLTSPT
                     VPVTAYRDLFGNWCSRIVAPAGRFVVSTDALLHDDGQPDLVAPWVMQTPVELLPDSAL
                     VFLLGSRYCETDQLNEIAWQLFGHGPTGWARVQAICEFVHQHIEFGYQHARRTRTALE
                     AYNEGQGVCRDYAHLAIAFCRCMNIPARYCTGYLADIRIPPIPDPMDFAGWFEAYLGD
                     RWYTFDARNNTPRIGRVLMARGRDACDVALASTFGPNTLTSFTVWADEVAVA"
     gene            complement(2442..4370)
                     /locus_tag="PLB25_08415"
     CDS             complement(2442..4370)
                     /locus_tag="PLB25_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014250802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HOE41635.1"
                     /translation="MRKKVVSATQAIAILRDGDTLCCSGFGSNGVPDELILALEKRFQ
                     ETGSPRDLTLLFGGGPGDGAKGGANRLAHEGLLRRTIGGHYGMLPQIGRLALDGKIQA
                     YNLPLGVVSHLYRDIACGLPGTVSKVGLGTFVDPRQEGGKINRQTTEDIVSVIELGGQ
                     ELLFYKTLPISVAFIRGTSADAEGNITMERETLTQDVLAIATATKNSGGFVVAQVERI
                     VERGSLHPRQVKVPGILVDCVVVARPENHLQNLGGSYNPAFAAEVRVPLDAMAPMLLD
                     ERKVIARRAAFELLPNAVVNLGIGMPEGVAAVANEEKILKYMTLTAEPGVIGGVPASG
                     INFGAATNPEAVIDMNQQFDFYDGGGLDLACLGLAECDPSGSINVSRFGPKLAGAGGF
                     INITQNSRSVVFVGTFTAGGLQVSMEDGLLKIVQEGRARKFVNRIEQVTFSGPYAAGS
                     GKNVLYVTERCVFKLTLQGLELIEVAPGVDIGRDILAQMDFRPIINNPQTMDPRIFLP
                     EPMRLADTLLSISLVDRMQFDVVKDTGYYNFQGLRVHSKKDVEDIRYAAEELCRPLGR
                     KIALVVNYDNFQIDEAVVDDYSAMVKEISDAYYTEVSRYTTSAFMRLKLGAALEKRGL
                     AAHIYETPKEAFESARQS"
     gene            complement(4672..5748)
                     /locus_tag="PLB25_08420"
     CDS             complement(4672..5748)
                     /locus_tag="PLB25_08420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41636.1"
                     /translation="MSDPTQAICCVATRDFLPGLILLYRSVLRHQRLPLYLLAVAVDP
                     EETRALTVALQAQVPQAEAGFIHIVSDRDIYGDALVQMRFYYDAFELAIAAKAGIHAW
                     MLAHTEVQRWLYLDTDIVCHGPLQPMFAQLDAHSLLLTPHVSQPCASPAEDLQYLAAG
                     AYNGGVIGVKRSATASAFAGWYVRVLEHLCLNDLPLPAKDRVSQSGGLFVDQRWLDLV
                     PAYFRDVAVATQRGFNLGHWNIGTDRLREQPGALYIADDPVTLLHLSGWMDDEPRRMS
                     RYSAIDWSDSPVWQRLHADYRASLLPLRVAFQYTYPYATYPDGSTVPKAHRRAYLKHL
                     LAGGARVPDPFLHKGDFERACAQA"
     gene            5834..6070
                     /locus_tag="PLB25_08425"
     CDS             5834..6070
                     /locus_tag="PLB25_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41637.1"
                     /translation="MKHSIAPEASRLSAQGSGLDRLSAFAGRLTTKMAAQGTLGMAGI
                     GQYQPELARARWMPRLTVVIPGFIAAAACAVALT"
     gene            6343..7215
                     /locus_tag="PLB25_08430"
     CDS             6343..7215
                     /locus_tag="PLB25_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41638.1"
                     /translation="MTCHFKQLVVVSLLALSAVQAPAVGRLADVMVVDRTNGATLPVY
                     FHAGQHWIAGQPGSKYAISVCNKLGERLLAVTSVDGINVLSGAEAAWGQTGYVFGNHA
                     CYEITGWRKSDRDVAAFEFSASGDSYAALTGRPRELGVIGVALFRERRPEPVIAPPVF
                     QSRANADSIKESRAGSSPGAPAAPAPATQNESARKSAERSLSADSALSAPAAMPAPIA
                     KLGTAHGQREESVVSHTEFQRAQVRPNEVIQIRYESHDALVALGVIPRPTRRLPNAFP
                     MSGRDSYVPDPPAR"
     gene            7224..7820
                     /locus_tag="PLB25_08435"
     CDS             7224..7820
                     /locus_tag="PLB25_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOE41639.1"
                     /translation="MHSPASSTDATDESLLLRFAEGDMKAFESLYDRHEMGSWRFIFR
                     SVRDRQVADDLLQDVWFAVARQAAAYQPRAKFRTWLFTMARNRVVDHFRASRDMQSLD
                     AHDDADDQVASAFVAEPVANSGFGPVRQLESKQQALALLEAIETLPQEQREAFLLQAE
                     AGLGVADIAEATGVSFETAKSRLRYARSRLRTLLWEFA"
BASE COUNT         1564 a         2340 c         2426 g         1528 t
ORIGIN      
        1 gaagtaaatg ctcataagga cttacagatg aaaacaagcg gtcaactgag gaatctaggt
       61 tcaatgcaac tggcacagcc agggcagcgg cagcgctgcg gcctggtcga aaccacattg
      121 ccgccccggc cttcaccact gcaacggtgg cagttcgcga aagaccttgc gggtgccagc
      181 cagccaaagc tgcatcagcg cttcaaagtc ggcatcgcca cggtcgaagc ggcgcttcat
      241 ggtgaccttc aggctgtctt gggcgtagca cagcaaatcg atgggcatgc cgaccgagag
      301 gttgctgagc atggtggtgt caaacgacac cagtacgcac ttgctggcat cctccagcgt
      361 tgccgaagag gttaccacca ggtccaggat gggcttgccg tacttcgcct cgccggtttg
      421 cagaaatggc gtgtccgtgc cggcttcgat gaaattgcct tcagcataga tgcgaaacaa
      481 gcgcatgtct tcaccggcaa tctggccgcc cagaatgaac gacgcgttga atttgagtcc
      541 gccagactgt agatacttgc catcgcgccg ctcaaggtca cgcatggcat cggccaccag
      601 gttggccaca tcaaacatcg agccaacgct ccagatatgg ggcggcccgg ccgccgcacg
      661 ctggcgcagg atgttgatca ccgtttgcgt ggccgccagc ccgcccgaac tcagcaacac
      721 cagcacccgg tcaccggtac gctcaaacac cgtcatcttg caaaacctgg cgtaattgtc
      781 gacacccgcg tgggtgcgcg agtcgctggc gaagatcagc ccttcatcga gcttgacacc
      841 aacgcagtag gtcatgcgac aaattggtgc aaggttcagg ccacggcaac ttcgtcggcc
      901 cagacggtaa aactggtcaa ggtgttgggg ccaaaggtgc tggccagcgc cacgtcgcag
      961 gcatcgcggc cacgcgccat cagcacacgg ccaatgcgcg gggtgttgtt gcgcgcatca
     1021 aaggtgtacc agcggtcacc caaatacgcc tcgaaccagc cagcgaaatc catcggatcg
     1081 gggatcggcg gaatgcggat atcggccagg taaccggtgc agtagcgcgc cgggatgttc
     1141 atgcagcggc aaaaggcgat ggccaggtgc gcatagtcgc ggcacacgcc ctgcccttcg
     1201 ttgtaggcct ccagggcggt gcgggtgcgc cgcgcatgct gataaccaaa ctcgatgtgc
     1261 tggtgcacaa attcgcagat cgcctgcacc cgcgcccagc cggtcgggcc gtggccaaac
     1321 agttgccagg caatttcgtt gagttggtcg gtctcgcaat agcggctgcc gagcaggaac
     1381 accagcgctg aatcaggcaa cagttccacc ggtgtctgca tgacccaggg ggccaccagg
     1441 tcgggctgac cgtcgtcatg gagcaaggca tcggtactca ccacaaatcg ccctgccggg
     1501 gccacgatgc ggctgcacca gttgccaaaa aggtcgcggt aggcggtgac aggcacggtt
     1561 gggctggtca aaagcaggtc ggggcgcacc aagtctggcg cacgggaatg gtgaatgttg
     1621 agcgccagaa tcatcggcgt tgggtcgggg cattcgtaaa ccatctcgaa acccacgcgg
     1681 atttgcaagc tggcctgctg gcccgctgac ggggttgtgg ggggcaatgg atggctcaat
     1741 ggggcgcgcc ggtcgtaggt catggtcatc tctggttggt tggtgacggg gtggcggatg
     1801 aactgacgct tttagcagcg ctgtgaccca gtacattttg gggcaggtcg ctgagaactg
     1861 tcgggctgaa cttcagattg gctggcaacc ggctcgacgc aggtgctggc gcagtggcaa
     1921 ttggcggggt atttggcaat gatttcatgg tggctgaatg taggaaactg tgtgccctgg
     1981 tgtctgtgcg ctggcgaacg ctgcaatgcc gcgtatggcc atggactgcc tgggtagacg
     2041 cacagaccgt gccaaccgcc atcgaacggg tgcttgaacg accagatgcg cccgcgctga
     2101 gcgccgcgcc tgcagcgcca gcgtcagcgc cagcgccagc gccagcgcca gcgccagcgt
     2161 cagagttttc tggcttcagc cagcatggcc aaagatggtt tggctatctt ggcgggtgcc
     2221 agcgattttc gtggtgcgtc gaggccgcga gtttgatggc gaactgcctg cctcatccac
     2281 taagcctcaa gtgccaaaat ggcctctatg ccttgccctg catgcgctac cagctattta
     2341 aaacgtagta actcttgtgc ccggctacgg cctgtcggcg ctggcgcaca gccctgaacc
     2401 gggcggcgcg caggccatgc cgatggcctc agttgcgcca ctcatgactg ccgggcagat
     2461 tcaaaggctt cttttggcgt ttcataaatg tgcgctgcca gaccgcgttt ctccagggcg
     2521 gccccgagct tgaggcgcat gaacgcactg gtggtgtagc gcgacacctc ggtgtagtag
     2581 gcatcgctga tttccttgac catggctgaa tagtcgtcca ccaccgcctc gtcaatctgg
     2641 aaattgtcgt aattgaccac cagcgcaatt tttctgccaa gcggccggca cagttcttcg
     2701 gccgcgtagc ggatgtcttc cacatctttt ttgctgtgca cccgcaggcc ctgaaagttg
     2761 taatagccgg tgtctttgac cacatcaaat tgcatgcggt ccaccaggct gatcgacagc
     2821 agggtatcgg ccaagcgcat tggctcgggc aggaagatgc gcggatccat ggtctgggga
     2881 ttgttgatga tgggcctaaa gtccatctgc gccaggatgt cacgcccgat gtccacgccg
     2941 ggtgcgactt cgatcagttc aagcccctgc aaggtcagct tgaaaacgca gcgttcggtc
     3001 acatacaaca cgtttttccc gctgccagcg gcataggggc cgctgaaggt gacctgctca
     3061 atccggttga caaacttgcg cgcacggccc tcctggacga ttttcagcaa gccgtcctcc
     3121 atgctgactt gcaggccacc ggccgtgaag gtgccgacaa acaccaccga gcggctgttc
     3181 tgggtgatgt tgatgaagcc accggcaccg gccagcttgg ggccaaaacg gctgacgttg
     3241 atgctgccag acggatcaca ttcggccagc cccaggcagg ccaggtcgag cccgccgcca
     3301 tcgtagaaat caaactgctg gttcatgtcg atcaccgcct cggggttggt ggcggcacca
     3361 aagttgatgc cagaggctgg tacgccacca atcacgcctg gctcggcggt gagggtcatg
     3421 tatttcagaa tcttttcttc attggccacg gctgccacgc cctcgggcat gccaatgccc
     3481 aaattgacca ccgcattggg cagcagctca aaggcggcgc gccgggcaat caccttgcgc
     3541 tcatcaagca gcatcggtgc catggcatcc agcggcaccc gcacctcggc cgcaaacgcc
     3601 gggttgtagc tgccaccgag gttctgcagg tggttttcgg gccgggccac caccacacag
     3661 tccaccagaa tgcccggcac cttgacctgg cgcgggtgca gcgaaccgcg ctcgacgatg
     3721 cgttcaacct gggccacgac aaagccgccg ctgtttttgg tggcggtggc aattgccagc
     3781 acgtcctgcg tgagggtttc gcgctccatg gtgatgttgc cctcggcatc ggcgctggtg
     3841 ccgcgaatga aggctaccga gatcggcagg gtcttgtaaa acagcagttc ctggccacct
     3901 agctcgatca cgctgacgat gtcttcggtg gtctggcgat tgatcttgcc gccttcctgg
     3961 cgcggatcga caaaagtgcc caggccaact ttgctgacgg tgcccggcaa gccgcaggcg
     4021 atgtcgcggt acagatggga caccaccccc agcggcaggt tgtaagcctg gatcttgccg
     4081 tcaagcgcca gtcggccaat ctgcggcagc atgccatagt ggccgccaat ggtgcgccgc
     4141 aacaggccct cgtgcgccaa acggttggct cctcccttgg ccccatcgcc cgggccgcca
     4201 ccaaacagca gggtcaggtc acgcggtgag ccggtttcct gaaagcgctt ttccagcgcc
     4261 agaatcagct catccggtac gccattgcta ccaaaaccag agcaacacag ggtgtcgccg
     4321 tcgcgcagaa tggcaatggc ttgggttgcc gagacgactt tttttcgcat ggtgatggct
     4381 cttttgtggt tggatggatt gactgtagca ctgcccaagc ggcagtgaca tattctgctg
     4441 ctgcgccact tccggtgact tcgcgctggc gtcagttggc ttgctggtgg cgggttgaca
     4501 tgcgccgggt ggccaaaatg gcaaacctgt gcgtctggtg atcgcgctgg gcagggggtg
     4561 gttgcgcctg cctcgttcac caggacggca ccgggtggcc ctgtgcggtg ctagcgcaac
     4621 cccgtcgctg cgtcagcggc tgcgtcagtc gctgcgcaca cggcacggcc atcaggcctg
     4681 cgcgcaggcc cgttcgaaat cgcccttgtg caagaacgga tctggcaccc gagccccgcc
     4741 agccagaagg tgcttgaggt aagcgcggcg gtgcgccttc ggcacggtgc tgccatccgg
     4801 gtaggtagcg tagggatagg tgtactggaa cgccactcgc aagggcaata aagacgcgcg
     4861 gtaatcagcg tgaaggcgct gccacaccgg tgagtccgac cagtcaatgg ctgaataccg
     4921 ggacatccgc cttggctcat cgtccatcca gccactcaaa tgaagcaagg tcacagggtc
     4981 gtcagcaatg tagagagccc ccggctgctc gcgcaggcgg tcggtgccaa tattccagtg
     5041 ccccaggttg aaaccgcgct gggtggcgac agcgacatcc ctgaaatagg cgggtaccag
     5101 atccagccag cgttgatcaa caaacaggcc gccggactgt gacacacggt cctttgcagg
     5161 caagggcagg tcgttcagac acaaatgctc cagcactcgc acataccatc cggcaaaggc
     5221 cgatgcagtg gcggagcgtt tcaccccaat cacgcccccg ttataggccc cggcagcgag
     5281 gtattgcagg tcttcagcag ggctggcgca aggctgggaa acgtggggcg taagcagcag
     5341 gctgtgggca tcgagctgcg cgaacatggg ttgcaacggc ccgtggcaaa cgatgtcggt
     5401 gtcgaggtaa agccagcgtt gcacctcggt gtgtgccagc atccaggcgt gaatcccggc
     5461 cttggcggcg attgccagct caaaagcatc gtaatagaaa cgcatctgga caagcgcatc
     5521 accatagatg tcgcgatcac tgacgatatg gatgaaccct gcctcggcct gcggaacctg
     5581 ggcctgcaag gcaaccgtca aggcccgcgt ttcttcagga tcgacagcca cggccagcag
     5641 gtaaaggggc aggcgctgat gtctgagcac actccggtag agaaggatca ggcccggcag
     5701 gaaatctcgc gtagcgacac agcaaatggc ctgggtaggg tcggacatgg tgctcctcgt
     5761 cagtgtgaac agaccgtggc gatcaccagc accaggccat tgcgttgaga gggccaactg
     5821 gcgttgccat cacatgaagc acagtatcgc acccgaggcc agtcgcttgt cggcacaagg
     5881 aagcgggctg gacaggctca gcgccttcgc tggcaggctc accaccaaaa tggcagcgca
     5941 aggtactttg ggcatggcgg gcatcgggca gtaccagcca gagctggccc gcgcgcgctg
     6001 gatgccccgc cttacggttg tcatacccgg ttttattgcc gctgcggctt gtgctgtggc
     6061 gttaacttag ttccaaataa atagcatgtc acgcatattc cataagggct agaggccatt
     6121 ttgcctgaca aatgcgagct ttcggtgccg ctggcatccc tttgccttta gatcaactga
     6181 cctgcctcgg cacccgcatc aatcgctgcc tccagaggcc gcagcgaagt tgtctgcttc
     6241 aatgctgttg gcggtgagca aaaataaaat tgagctcctt aaccccattt ccatctgacc
     6301 tccgtttcag tcagttccta tctttctttt ttggagttaa acatgacctg ccatttcaaa
     6361 cagcttgttg tcgtaagcct tctggccctg tctgcagttc aggcgccggc ggttggtcgc
     6421 ctggcggatg tgatggtggt tgaccggacc aacggtgcca ccctgccggt ttattttcat
     6481 gccgggcaac actggattgc tggtcagccg ggttccaagt acgccatttc tgtgtgcaac
     6541 aagcttggtg agcggttgct ggcagtgact tcagttgacg gcatcaatgt gctcagcggt
     6601 gccgaggcgg cctgggggca gactggttat gtgtttggca accacgcctg ctatgaaatc
     6661 accggctggc gcaagagcga cagagatgtt gctgccttcg agttttccgc atctggcgac
     6721 tcgtatgcgg cgttgaccgg tcgtcccaga gaactggggg tgatcggtgt cgcgctgttt
     6781 cgggaacgtc ggccagaacc agtcattgcg ccaccggtgt ttcaaagccg cgcgaatgcc
     6841 gacagcatca aggaatcgcg cgctggcagc agccccggcg cgccggcagc gccagcacca
     6901 gccacgcaaa atgaaagcgc aaggaagagt gccgaacgca gccttagtgc cgacagcgcc
     6961 ttgtcggcac ctgcggccat gcccgcgccg atagccaagc ttggtacggc ccacggccag
     7021 cgcgaggaat cagtggtatc gcacaccgaa tttcagcgcg cccaggttcg ccccaacgaa
     7081 gtgattcaga ttcgctatga aagccacgat gctttggtgg ccttaggcgt gatcccgaga
     7141 cccacacgcc gcttgcccaa cgcgtttccc atgtccggtc gtgattcgta tgtaccagac
     7201 ccgccagccc gataattcgg gccatgcaca gcccagccag ctcaaccgac gcaaccgacg
     7261 agtcgctgct gctgcgcttt gccgaaggtg acatgaaagc ttttgagtcg ctctatgacc
     7321 ggcatgaaat gggctcgtgg cgcttcattt ttcgcagtgt ccgggaccgg caagtggccg
     7381 acgatctgtt gcaggatgtc tggtttgcgg tggcgcgcca ggcagcggcg taccagccca
     7441 gggcgaaatt tcgcacctgg ctgtttacca tggcgcgcaa tcgggtggtt gaccactttc
     7501 gggcctccag ggacatgcaa agcctggacg cgcacgacga tgccgacgac caggtggctt
     7561 cagcctttgt tgctgagccg gttgccaatt cgggctttgg cccggtgcga caactggagt
     7621 ccaaacagca agcgctggcc ctgctcgaag ccatcgaaac gctgccgcaa gagcaacggg
     7681 aagcgttctt gctgcaggcc gaagcgggac tgggggtggc cgacattgcc gaggcaactg
     7741 gtgtgagttt cgagaccgcc aaaagccgct tgcgttatgc ccgcagtcgc ttgcgcacgc
     7801 ttctctggga gtttgcatga ccaatgccga tgacgacttg cagcagatct accggcag
//
LOCUS       DAOSLL010000151         7847 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8565_length_7847_cov_4.798255, whole genome shotgun sequence.
ACCESSION   DAOSLL010000151 DAOSLL010000000
VERSION     DAOSLL010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7847)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7847)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7847
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8565_length_7847_cov_4.798255"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PLB25_08440"
     CDS             complement(<1..334)
                     /locus_tag="PLB25_08440"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HOE41640.1"
                     /translation="MNRLAAALLWFFFGAGLAGPAVADVWKVVDENGVIQFTNEPPGK
                     KGQLVISSNAPGPAHATVASQAGSSGGATLATVAGINASANYQAVHASLSAASQSYGV
                     DYELLKAVV"
     gene            424..2442
                     /locus_tag="PLB25_08445"
     CDS             424..2442
                     /locus_tag="PLB25_08445"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE41641.1"
                     /translation="MTPKLSFALYAIALAAAIAFVYSFSLNNQLVFDDERLTDGTVFG
                     QYGSLMQLKARTLSYGSFVWVKQILGDGWWKQRVFNIALHIGTALILYALVLDLLGRT
                     QWNLNDRQKADASRFASSLRNAARAGVALWAFNPVAVYAVAYLIQRSILMATLFVALA
                     CLGFVRGLVSGRRYWHLVALVSYALAAASKEHAISAIFLTVPLFVFVKRPPLKRIAQV
                     AGATAVVLGAAFAGALLFTQYGSIVGSVFDEISRAHAIHLEKQWPGISQKLYPLSIIN
                     QASLFFHYGLLWLVPYVGWMSIDMRPAFPVAFISAQLVGAVGWLGLLLGSAWLVLRRT
                     DAWALAGLALLVPALLFVTEFLTVWLQDPLVLYRSYLWSIGIPILLALPLVGRSDRKL
                     YALVLIAVGVLAAGTFERIQSLSSPTSTWVDASAKIDLQAPVNAVGRWRPVLNQGEAA
                     MFRGNFDEALRLFTQAEALGENLGESARLRTQVAQRQQLANHAKILDQLAVVQAKGLD
                     QAALLFERGESEYGLARYADAFESFSKALTHRQAPQAEELTRLRQAEAAIAIQKYDEA
                     IASYRLLIKQSPDNQRYQVGLSVALVGKQDYQAALSILNPAIAKDPTGPAHYARALTH
                     FYMGDRTASKQDIDIALRAEPGNPVYRQLLNTLNDSTAQPTAMPANKP"
     gene            2442..2795
                     /locus_tag="PLB25_08450"
     CDS             2442..2795
                     /locus_tag="PLB25_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2304 domain-containing protein"
                     /protein_id="HOE41642.1"
                     /translation="MANLQITTSLLGLGLALFILHLLRRDHIYILHGLFWIAVASLAA
                     VLGLWPGLIDRIASWVGISYSPAALLLGAVLVLFVKILYADMTQTRLERQLRRLNQRI
                     ALMDAQKPLPDDQSP"
     gene            2977..5409
                     /locus_tag="PLB25_08455"
     CDS             2977..5409
                     /locus_tag="PLB25_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HOE41643.1"
                     /translation="MNQPCHRDRYALDPTKCMRLLACALLLWLTGAQYALATAERVVL
                     GNSLAIPLAASTDPSVSNALDKLRKKMAQTTAMRVIVGVRVPFAPEGLLAAASSAQQR
                     IDIAAGHRAVLGKIPALAEKTDKSRLFETIPFMALEVTPKELEELAALTEVTSIIEDR
                     PASFLLAESVPLVGGTSAWASGYTGAGQVVAVLDTGVDKTHTFLTGKVVSEACYSTNA
                     TNVVSICPGGVTSSTSPNSALPYSSGVCPSGQCDHGTHVAGIAAGKSASFSGVAKEAS
                     VIAVQVFSRFNSSTDCGGSSPCVMSYSSDQISGLERVYALRNDYSIAAANMSLGGGSY
                     SSQTDCDLDNYAIKASIDNLRAARIATVIASGNSSYTSSMGGPGCVSSAVSVGATWDS
                     GAVNTVASYSNSVSFLNLLAPGSMINSSVPTPANSFSSWQGTSMATPHVAGAWAVLKH
                     KASTATVNEVLAAFSSSGLAVTDSRNGITKPRINLPAALTALTPGVQFSLSVSKAGAG
                     TGSVVSSPPGIDCGAGCVASFSSGTAVTLTATPSGSSIFTGWSDACAGTTCNLTMSTA
                     LSATATFVPGMIVTPINQANLSGSSGNSQYFSVVVPVGAANLVIQTSGGTGDADLYVK
                     AGSQPTTAVYDCRPYLPGNAETCTFGSPLATTYHILLRAYSAYSGVTLTAKYTTAAPV
                     YPGAFNITAASMTVRENAGNAVISVTRTEGSDGAASVAYSTSAGSATAGVDFSTASGT
                     LIWGGGDASTRTFSVPIINNTVRNHTPRSFSASLSSASVARLGTVTSITVNILDDEVI
                     AMPWLNLLLLDD"
     gene            complement(5537..5755)
                     /locus_tag="PLB25_08460"
     CDS             complement(5537..5755)
                     /locus_tag="PLB25_08460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HOE41644.1"
                     /translation="MGMEWIKPGVLLGSLVYALLGVIIFWLCFIIIDKITPYDLWEEI
                     VEKQNQALGLVVAAMSLGICIIVAAAIH"
     gene            complement(5793..7412)
                     /locus_tag="PLB25_08465"
     CDS             complement(5793..7412)
                     /locus_tag="PLB25_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HOE41645.1"
                     /translation="MASTATQRAYRAPCPGCGAPVEFASAQSTHAVCGFCQSAVVRQG
                     ETLARIGKMAELFDDHSPLQLQASGIWHGQAFTLVGRLQYKYAEGTWTEWYAVLADGS
                     SAYLSEDNGAYVMAVAQNLSSELPPAARFRLGASTAINGQTFSVASNQQVLLLSAQGE
                     LPHLPTLGQPFDMVELRSQGQVTAQKAAASAVPPGADGSSMIGSNLGPAADAATGTGQ
                     SGGRVLSIDYSTRPPTVTLGEPVELEALKLTGLRDESVKTEAARQFNCPNCGAPVAVT
                     LASSQSVTCGSCNSLIDVSGGIGGELKHALQDEPVQALIALGSIGQLQGVAWQVVGFQ
                     HRMGTDPSDPDEQFGWEEYLLYNQKRGFSFLVDSTEGWSLVKPATGAPVLSANGQTAT
                     YLGTRYQLKESYAAETSYALGEFYWQVTRGQKTSNRDFASGAKLLSMEQSPTEVVWSV
                     GGKIGSDAVAKAFKLEDKKDLLKRSDVGPTSSGKAMSVRTLIVIFLLLILMSIIFSRC
                     SSCDPKVENCSSSSRSSGGSFGGFSGGGGHK"
     gene            complement(7453..>7847)
                     /locus_tag="PLB25_08470"
     CDS             complement(7453..>7847)
                     /locus_tag="PLB25_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOE41646.1"
                     /translation="VSLPEELQKILDQKIGMGMIGNDMGKFMQYQTAQAIPKFAEGGG
                     GGGSGIAGDAMGLGAGVALGQVLAQNLAAGLQPQAAPAAAAGAAPVGMKAEDVMATLE
                     KLGELKSKGILTQDEFDAKKAELLKKLV"
BASE COUNT         1525 a         2456 c         2236 g         1630 t
ORIGIN      
        1 ccaccaccgc cttgagcagc tcgtaatcca cgccgtaaga ctgcgatgcc gccgataaac
       61 tggcatgcac cgcctggtaa ttggctgacg cgttgatgcc cgctaccgtg gcaagcgtcg
      121 cgccgccaga cgaaccggcc tggctcgcca cggtggcatg ggctggcccc ggcgcgttgg
      181 atgagatcac caactggcct tttttgcccg gcggctcatt ggtgaactga atcacaccgt
      241 tttcatccac caccttccag acatcggcca cagccgggcc cgccagtccg gcgccaaaaa
      301 aaaaccacag caaagctgcg gccaaacggt tcacatcaat tgaaaaaaac aaagcaaaaa
      361 cccctatcaa gcgagcacct acaatccatt tcccattcaa tctggacaaa ttcagggtca
      421 ggcatgacac caaaattatc ctttgcgctt tacgccatcg cacttgcggc agcgattgcc
      481 tttgtttaca gcttcagcct caataaccaa ctggtgtttg atgatgagcg cctgacggac
      541 ggcaccgttt ttggccaata cggcagtctg atgcaactca aggcgcggac gctgtcgtat
      601 ggcagttttg tctgggtaaa gcagatttta ggcgatggct ggtggaagca acgcgttttc
      661 aacattgcat tgcacattgg cacggcgctg atcctctatg cactggtgct tgacttgctt
      721 gggcgaaccc agtggaactt gaacgacagg caaaaagcgg atgccagccg ttttgccagc
      781 tctttgcgca atgccgcgcg tgccggggtt gccctgtggg cattcaaccc ggtggcggtg
      841 tatgccgtgg cttacttgat tcagcgctcg atcctgatgg cgaccctgtt tgttgcgctg
      901 gcttgccttg gctttgtgcg cggcctggtc agcggtcgcc ggtattggca tctggtcgct
      961 ttggtcagtt acgcgctggc agccgcttcc aaagagcatg ccatcagcgc cattttcctg
     1021 acggtgccac tgtttgtttt tgtcaaacgg ccgccgttga agcgcattgc ccaggtcgcc
     1081 ggcgcgaccg cagtggtgct gggggcggcg tttgccggcg cactgctgtt tacccagtat
     1141 ggctcgattg tcggttcggt ctttgatgaa atttcgcgtg cacatgccat ccatcttgaa
     1201 aagcaatggc ccggcatcag ccagaaactt tacccgttga gcatcatcaa tcaggcatcg
     1261 ttgttttttc actatgggct gttgtggctt gtgccctacg tgggctggat gtcgattgac
     1321 atgcggccag cctttccagt cgctttcatc agcgcccagt tggtgggtgc cgtgggctgg
     1381 cttggcctgc tgttgggcag cgcctggctg gtgctgcgtc gaaccgatgc ctgggctctt
     1441 gctggtctgg ccttgctggt gccggctttg ctgtttgtga ccgagttcct cacggtttgg
     1501 ctgcaagatc cgctggtgct ttaccgcagc tatttatggt caattggtat cccgatcttg
     1561 ctggctctgc cgctggtagg tcgcagtgac cgaaagctct atgcgctggt gctgattgcc
     1621 gtcggggtgc tggcggccgg tacgttcgag cgcattcaaa gtctgagttc gccaaccagc
     1681 acctgggtcg atgccagtgc caagattgat ctgcaggcac ccgtcaacgc tgtgggccgc
     1741 tggcgaccgg tattgaacca gggtgaagcg gccatgttcc gtggcaactt tgacgaggcc
     1801 ctgcgcctgt tcacccaggc cgaagcgctg ggtgagaacc tgggcgagtc ggcacgcttg
     1861 agaacgcaag ttgctcagcg acagcaactg gccaatcacg ccaagatact cgaccaactg
     1921 gccgttgtac aggccaaggg ccttgaccag gctgcgttgc tgtttgagcg cggcgaatca
     1981 gaatatggtc tggcacgcta tgccgacgct tttgagagct tctccaaagc cctgacccac
     2041 cggcaagcgc cacaggctga agaactgacc cggctgcgcc aggccgaggc ggcgattgcc
     2101 attcaaaaat acgatgaagc gattgccagc tatcggctgc tcatcaagca aagccctgac
     2161 aatcagcgct accaggtggg cttgagcgtg gccctcgtgg gcaagcaaga ctaccaggcc
     2221 gccctgagta ttttgaaccc ggccattgcc aaagatccga ccggtccagc ccactatgcg
     2281 cgcgccctga cacactttta tatgggtgac cgcaccgcca gtaaacagga cattgacatc
     2341 gccctgcgtg cagagcctgg caacccggtg taccggcaat tgctcaacac cttgaacgac
     2401 tcaactgcgc aacccactgc catgccggca aacaaaccct gatggccaat ctgcaaatca
     2461 ccaccagcct gctcggcctg ggcctggcat tgttcatcct gcacctgttg cgacgtgacc
     2521 atatctacat tcttcatggt ctgttctgga tcgctgtggc atctttggcc gccgtgctcg
     2581 gcctttggcc cggcctgatc gaccggattg caagttgggt cggcatcagc tactcaccgg
     2641 ctgcactgct gctgggtgcc gtcctggttc tgtttgtcaa gatcctgtac gctgacatga
     2701 cccagacccg gcttgagcgc cagttgcggc ggctcaatca gcgaatcgcc ttgatggacg
     2761 cgcaaaagcc gttgccagat gatcaatccc cttgagagcg agcatcagcc tggctttgga
     2821 tgcctgcatc atctggcatg cccctgacca gacccgcccc gtccattgcc caaaaagtca
     2881 ctcaaacggt ggcgtggccg cgcgccagaa taatcaatag ccagagtggt tgattgattt
     2941 cataattggc ttcttatcgt tccacttgaa gccgaaatga atcagccctg tcaccgcgac
     3001 cgctacgccc ttgacccgac aaaatgcatg cgattgctgg cttgtgcgct gctgctgtgg
     3061 ttgaccggtg cacagtacgc gctagccact gcagagcgtg tcgtgctggg taattcgctg
     3121 gccattccgc ttgccgccag caccgacccg agcgtcagca atgcgcttga caagctgcgc
     3181 aaaaaaatgg cacaaacaac agccatgcgc gtcattgttg gcgtgcgcgt gccctttgcc
     3241 cccgagggct tgctcgccgc cgcaagttcg gcgcagcagc gcatagacat cgctgcaggg
     3301 caccgggcgg tgctgggcaa aatacccgcg ctggccgaga aaaccgacaa gtcccgactt
     3361 tttgagacca ttccgttcat ggcacttgag gttacgccaa aagaactgga ggagctggcc
     3421 gccctgactg aagtcacctc gatcatcgaa gaccgtcccg caagctttct gctggctgaa
     3481 agcgtgccac tggtgggcgg cacaagcgcc tgggccagcg gctacaccgg ggctggtcag
     3541 gttgttgccg tgcttgacac cggtgttgat aaaacgcaca ccttcctgac cggcaaagtg
     3601 gtgtcagagg cgtgttactc gaccaatgcc accaatgtcg tgtcgatctg ccctggcggg
     3661 gtgacatcat ccaccagccc caactcggcc ttgccctact caagtggcgt ttgtcccagt
     3721 ggccagtgcg accacggcac ccatgtggcg ggcattgcgg ccgggaaaag tgccagtttt
     3781 tccggtgtgg ccaaggaggc atcggtcatc gccgtacaag tgtttagccg ctttaacagc
     3841 agcactgatt gcggtggctc aagcccctgc gtcatgtctt acagttctga ccagatcagc
     3901 ggcctggagc gggtctatgc cttgcgtaac gactacagca ttgccgctgc caacatgagc
     3961 ctgggcggcg gcagctacag cagccaaacc gattgcgacc tggacaacta tgccatcaag
     4021 gcaagcattg acaacctgcg tgccgccagg attgccaccg tcatcgcctc gggcaacagc
     4081 agctacacca gcagcatggg tgggccgggt tgtgtttcca gcgccgtcag cgtcggtgcc
     4141 acctgggatt caggggcggt caacacggtt gcctcatact ccaattcagt ttcgttcctg
     4201 aacctgctgg caccgggctc gatgatcaac tcatcggtac caacaccggc caacagtttc
     4261 tcaagctggc aaggcacgtc catggcaaca ccccatgtag cgggggcctg ggccgtgctc
     4321 aagcacaagg catccacggc gacggtcaac gaggtgctgg cggcgttcag ttcaagcggg
     4381 ctggcggtta ccgacagccg caacggcatc accaagccac gcatcaacct gcctgccgcc
     4441 ctgacagcac tgacaccggg tgtgcagttc agcctgagcg tcagcaaggc gggtgctggc
     4501 actggcagcg tcgtcagttc gccgcccggc attgactgtg gtgctggctg cgtggccagt
     4561 ttttcgagcg gcacggcggt caccctgacg gccacaccat ccggcagcag tatttttacc
     4621 ggctggagcg atgcctgtgc cggcaccact tgcaacctga cgatgagtac tgcactcagc
     4681 gccaccgcca catttgttcc cggcatgatc gtgaccccaa tcaaccaggc caacctgtcg
     4741 ggcagcagcg gcaattcaca gtacttttct gtggtggtgc cggtgggcgc ggccaatctg
     4801 gtcattcaaa ccagtggcgg cacgggtgat gcggacttgt atgtcaaggc aggcagtcaa
     4861 ccaaccacgg cggtttacga ctgcaggccc tacttgccag gcaatgcgga aacctgcacc
     4921 tttggcagcc cgttggccac cacctaccac attttgctgc gcgcctattc ggcttacagc
     4981 ggggtaactt tgacggcgaa atacaccacc gcagcacccg tctatcctgg cgctttcaat
     5041 atcaccgccg ccagcatgac ggtcagagag aacgcgggca acgccgtcat cagcgtgacg
     5101 cgcaccgaag gcagcgacgg cgcagccagc gtggcttaca gcaccagtgc tggcagcgcc
     5161 accgcgggcg tggacttcag cactgcttcg ggcactctta tctggggtgg cggagatgcc
     5221 agcacgcgca ccttcagcgt gccgatcatc aacaataccg ttcgcaacca tacaccccgc
     5281 agtttcagcg ccagtctgag cagcgcgtcg gtggcccgcc tgggcactgt caccagcatc
     5341 accgtcaata tccttgacga tgaggtgatc gccatgccct ggctcaatct gttgttgctt
     5401 gacgactgaa cctgcccagg ggcgagcccc gacaaggccg catccatggc ccttggcggc
     5461 cctctgggga tttcattttc tatagcatgc tacgcttgct tgacaagggc tgcagcctat
     5521 tttctcaatg aatttctcaa tgaatcgcag ccgccacaat gatgcagatg ccgagcgaca
     5581 tggcggctac caccaggccc agtgcctggt tctgcttttc gacgatttct tcccacaggt
     5641 catagggcgt gatcttgtcg atgatgatga aacacagcca gaagatgatc acacccaaca
     5701 aggcatacac cagcgagccc agcaaaacgc ccggtttgat ccattccata cccatgctga
     5761 tctcctttaa aaatgtaaca agcggcgctg catcacttgt gcccgccgcc gccggaaaaa
     5821 ccgccaaacg agccacctga actgcgtgaa ctgctggaac agttctccac cttggggtcg
     5881 caactcgaac agcggctgaa aatgatcgac atcaggatca acagcaggaa gatgacgatc
     5941 aaggtgcgca cactcatcgc cttgccgctg ctggtcggac cgacatcgct gcgtttgagc
     6001 aagtcctttt tgtcttcaag cttgaaggct ttggcgaccg cgtcgctgcc aatcttgcca
     6061 ccgaccgacc agaccacctc ggtgggcgac tgctccatcg acagcagctt ggcgccgctg
     6121 gcaaagtcgc ggttgctggt tttctggccg cgcgttacct gccagtaaaa ctcgccgagc
     6181 gcgtagctgg tttcagcagc gtagctttcc ttgagctggt aacgggtgcc cagataagtg
     6241 gcggtctggc cgttggcact cagcaccggc gcaccagtgg cgggcttgac caggctccag
     6301 ccctcggtcg aatcgaccaa aaagctaaaa ccgcgcttct ggttatagag caaatactct
     6361 tcccagccaa actgttcgtc gggatcgctc gggtcagtgc ccatgcggtg ctggaaaccc
     6421 accacctgcc aggccacccc ctgcaactgc ccgatgctgc ccagggcaat caaggcttgc
     6481 accggttcat cctgcaaggc gtgcttgagt tcgccgccga tgccgcccga cacatcaatc
     6541 aggctgttgc aggaaccgca ggtgacactc tggctgctgg ccagggtcac tgccaccggg
     6601 gcaccgcagt tggggcaatt gaactggcga gcagcctcgg tcttgaccga ttcatcgcgc
     6661 aagccggtca gcttgagcgc ctccagttca accggttcac ccagcgtgac ggtgggcggc
     6721 cgggtgctgt agtcaatgct cagcacccgg ccaccggatt gccctgtgcc ggtcgctgcg
     6781 tccgctgcag gcccgagatt gctgccaatc atgctgctgc catccgcgcc tggcggcacg
     6841 gcggatgcag cggccttttg cgcagtgacc tgtccctggc tgcgcagctc gaccatgtca
     6901 aacggttgac ccagcgtcgg caggtgtggc agctcgccct gcgcagacag cagcaacacc
     6961 tgctgattgg aggccacgct gaaggtctgg ccgttgatgg cggtgctggc acccaggcga
     7021 aaacgcgcgg ctggtggcag ctcgctcgac aggttttgag cgaccgccat cacgtacgcg
     7081 ccgttgtcct cgctcagata ggcgctgctg ccatctgcca gcaccgcgta ccactcggtc
     7141 caggtgccct cggcgtactt gtattgcagt cgcccgacca gtgtgaaggc ctggccgtgc
     7201 cagatgccgc tggcttgcaa ctgcagcggg ctgtggtcgt cgaacagctc ggccatcttg
     7261 ccgatgcgcg ccagtgtctc gccctgtcgc accaccgcgc tctggcaaaa gccgcagacc
     7321 gcatgggtgg actgggcact ggcaaattca accggtgcgc cgcacccagg gcagggagcc
     7381 cggtaggcgc gttgggtggc agtgctagcc atcggaaccc gtcagcacaa agttggctgc
     7441 aagccgacgt tgctaaacca gcttcttcag caattcggcc tttttggcat caaactcgtc
     7501 ttgcgtcagg atgcctttgg acttgagttc accgagcttt tccaaagtcg ccatcacatc
     7561 ttcggccttc atgcccaccg gagccgcgcc agccgctgct gccggtgccg cctggggctg
     7621 caaaccagca gccagattct gcgccagcac ctggcccagc gcgacgcctg cgcccaggcc
     7681 catcgcatca ccggcaatac cgctgccgcc gccacccccg ccttcagcaa acttcggaat
     7741 cgcctgtgcg gtctggtact gcatgaactt gcccatgtcg ttgccaatca ttcccatgcc
     7801 aatcttttgg tccagaatct tttgcagttc ctctggcagc gagacgt
//
LOCUS       DAOSLL010000152         7789 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8640_length_7789_cov_3.755107, whole genome shotgun sequence.
ACCESSION   DAOSLL010000152 DAOSLL010000000
VERSION     DAOSLL010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7789
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8640_length_7789_cov_3.755107"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(527..1990)
                     /locus_tag="PLB25_08475"
     CDS             complement(527..1990)
                     /locus_tag="PLB25_08475"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOE41647.1"
                     /translation="MQSDLAESNNIQKLISQIGVGLDQFLKFDHPDAIRPDANWREYL
                     DVSLPQHGVGIDQVVKDLLKQVIPNGSSIPKPGFTSFITTGATTASVLASTAASIASP
                     QRYGITAFNLLEDVSLRWLASMCQIGHMQGVYSSGGSVANLLALGAARQHAFEKLGHD
                     AGANGVTKPVHIYASAESHHTIQRSAGVLGIGRRAIQSIACDSQGRMRVDALRAAIEK
                     DRNSGIVQMAIVACAGTTNTGAIDPLHEIGDIAKENGIWFHVDGAYGLPGILDERIRH
                     LYDGLEMADSVIVDPHKWLGAAVGVAATFVRDRQLLYRAFTQEPADYLEGAIEQTTDS
                     APKIEHSLDDFGVPYYDFGVELSSPCRGVVVWAMIREIGVEGMTQRMMRHNDMATFIA
                     KAAKEHPNLELLLEPTLSICCFRYVAPGVADLDAFNRQLHRRLIRENQNMPSTTLVNG
                     KLALRPCFVGARTSHEHAQSLLNDVLRIGQELAPNLA"
     gene            2210..2467
                     /locus_tag="PLB25_08480"
     CDS             2210..2467
                     /locus_tag="PLB25_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41648.1"
                     /translation="MKSADPKITPFSLERLQKAITGHSTKLPNNYPKGESSPSISPEA
                     DLRFDTRLWGAVIQVLGLVGYSIYPQSVKSPIAMQYDSLFV"
     gene            2578..3795
                     /locus_tag="PLB25_08485"
     CDS             2578..3795
                     /locus_tag="PLB25_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41649.1"
                     /translation="MSSLDVEFNGFQFGTLTQLICSAGPIPVATVSSIGSDRLITRFE
                     WSPGLLVSTVSGVPVVAGTVIVQAPVRIHHLSAAELLANPAAAPSTTNATAWVRWSVD
                     RSGLLTGHFVRIDANGRGDVFSRPSEIGRQRFTVPRVGAASIASAALLWRDDVVTIRF
                     CTSITDDVLAQPLNSIADLGMAWGVRISDGLLVDMLREQLTTGLAKMPPGTVIEDPLT
                     VVWKQDEQGVWGASADVGIEKLDACPGLLGAVDISVALSTRLTFSPDLTPQRPKFPHG
                     RLGLRVVVESDTSDWDSFRCWAGIAPLVVFASPLFSGVGVIALFVGSLIAVSAFVAGK
                     VSDETANAPLGSDFQREPSGDDDVIAFVGAVELPQFPGQVDVAEVRSSGLVFAGGTFV
                     VVPALHHTTFTPD"
     gene            3865..4989
                     /locus_tag="PLB25_08490"
     CDS             3865..4989
                     /locus_tag="PLB25_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41650.1"
                     /translation="MQPIYVTDQAQLPDNPPGTGYPKLMPVTMFPTTSFSPTTITGWS
                     VAALRPTNQQIVTVVGKGSEIVGKPVVLILHTSAGLRRYEIAAPTDVPKSNQFEVELQ
                     QIACKHWGFREWAPRMPMKWRVDPPPYDFGFRPLRQWMVTFKSIPEGVSFELRTVHGD
                     DIDDGQVIVQRTTDATGEMFLDAVTDEQAELSLTNDLEHSPAGASLRQRWLLPTASSK
                     LAAQGLSVMRHGNAVVALTTDGATVLRYNTSSAKGPLLDLVKSFTGIDGVLRSTLGLV
                     IWGARGLFVVLDEANKIDFKRVTKAPIRAAGRGADGVVRVMQEDGVMELRDGFGLEPA
                     NTEVLGLVDAQSTSRPLPDGSVVAIDPDATIVLARPMRRF"
     gene            5277..6707
                     /locus_tag="PLB25_08495"
     CDS             5277..6707
                     /locus_tag="PLB25_08495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41651.1"
                     /translation="MNIDFTPRSITCHQTVSSPSAPWGMEDGFRLYFAVEVAFQSPDV
                     MISGRKYPEPDVVDNYPVKAGQTISLDTFPPVDRASWQTKVFNVDEGNLVTVWLIGVN
                     EGLPFFGGGGGGSQFSTKLASKGGQFLIKKIADQASEEALKRSIAGASEGLIKPVPIV
                     GALASALIELAEYIDESTDCSGSVFIYKKEILGRDLIAQLLQNRRSIMLSFNQNQSLR
                     TDSPDNPCLTPSYQATLDINLRSVLKVTPSLTLLKTITTAPVEFNPLFISCAKKTSEP
                     IQVWSEVRDFKIEFIPNFTFQLMPIIWKIENQVLTQQQSTGTFNLPVTIQPNQQNKLS
                     DVNIRYEIDLRQHLILSTTGSEGSFQIRVQAFLPLQSQQAPFLIYQHILVVDSQDMLG
                     NKGYQEYLDCMGAKLNGLITEIDKTQFVRQREFLPRQPFKQLVTSRSERAQKQIEAMG
                     LDDRISVSQTVNVREMEELLGFAIKL"
BASE COUNT         1763 a         2089 c         2089 g         1848 t
ORIGIN      
        1 tttagcggct cacaattctt aaagcccccg gctttgccgg gggatggtta ctcgtgattg
       61 tcacaaaaac aagggtacgt tcaaggccgc tatgggctgc gataaaacac acgtccgacc
      121 ggatcaattt ggttggcagg cggtaaggaa cagatcgttt cggaatggtt gacgcagatt
      181 gagggggcaa tcacggtcag gttacgcacc ctatgcggtc ataaaaggcg gcgctgccac
      241 tggctccaat gcagcggttg cggacggtcg cggtcagccc tggaatctca ttgatttatc
      301 caaagcggtc atgggcggtg ccggtccaat ttcgtcattg gcccttcttc aacactcgaa
      361 agcagccatt cgaagggcaa gatcgggttt tgccagtggc ggtcggcact gcagcgaccg
      421 gagtcattcg gattttgaca ggggatggct ccagaaaggc caatttctgt cattggcacg
      481 ttacaactgc tcgctccaaa gcggtcagtc aagagtgtca acccatctat gccaagttgg
      541 gggccaactc ttggccgatg cgcagaacat cattcaatag tgattgcgca tgctcatggc
      601 tggttcgcgc tccaacaaaa caagggcgca atgctagttt cccgtttacc aaagtagtgc
      661 tcggcatgtt ttgattttct cgaatcaacc ggcggtgcaa ctgccggtta aaagcatcca
      721 ggtcggcaac acctggagct acatatcgaa agcagcaaat tgacagagtt ggctcaagta
      781 gaagttcaag attgggatgt tccttggccg ctttggcgat aaaggtggcc atgtcgttat
      841 gacgcatcat ccgttgggtc attccttcaa cgccgatctc acggatcatg gcccacacga
      901 ccacaccccg acatggggag ctcaattcaa caccgaaatc gtagtaaggc acaccaaagt
      961 catcgagcga gtgctcaatt tttggtgctg aatctgttgt ctgctctatc gcgccttcca
     1021 ggtaatcagc aggctcttgg gtaaatgccc ggtataacaa ctgacgatca cgcacgaaag
     1081 tggccgcgac gcccacagca gcgcccaacc atttgtgtgg gtccacaatc acagagtccg
     1141 ccatctccag accgtcatat agatgacgga tacgttcatc caaaattcct ggcaggccat
     1201 aagcgccgtc aacgtgaaac caaatcccgt tttcctttgc gatatcacca atttcatgta
     1261 gcggatcaat cgcgccagta ttggtggtgc cagcgcaagc aacaatggcc atctgtacta
     1321 ttccggagtt cctgtctttt tcgatagcag cacgcaacgc atccacccgc attcggcctt
     1381 ggctgtcaca agcgatgctt tgtatggccc gccgtccgat tccaaggaca ccggcagatc
     1441 gttgaatcgt gtgatgcgac tcggcgcttg cgtaaatgtg gactggcttt gtaacaccat
     1501 tggcaccagc gtcatgcccc aatttttcaa atgcatgctg cctggcagca cccaatgcaa
     1561 gcaggttagc cacagaacca ccgctgctgt acacaccctg catgtgtcca atctgacaca
     1621 tagatgccaa ccagcgcagt gacacgtctt ccagaagatt gaacgccgta atcccgtagc
     1681 gttggggtga cgctatgcta gccgccgtgc tggccaacac tgatgcagtt gtggcgccgg
     1741 ttgtgatgaa agaagtgaag cccggctttg gtatcgatga tccgttggga atgacctgtt
     1801 ttaacaagtc ttttacgact tgatcaatac ctacaccgtg ttgaggcaaa gaaacgtcca
     1861 ggtattcccg ccaattcgcg tctggacgta tggcatctgg gtgatcaaat ttcaaaaact
     1921 ggtctagacc tactccaatt tgggagatta gcttttggat gttatttgat tcggcgagat
     1981 cagattgcat ttttaccttc aaaaatggtc aaagagacct caggttttta caggccatac
     2041 caaaacggcg ggttccaaca aatgaactgg aatgtgcaac gatcctaatc gttttcatca
     2101 tagagttctg ctacacatag catttcgcaa agcagccatt ggcgactaac tcctggcgct
     2161 ggcgtacttg actgccaaat aggccacatg aacgacagga atcagcctgt tgaagtcagc
     2221 cgacccgaaa attaccccgt tttcactcga aaggctccag aaggccatca ccggtcattc
     2281 aacaaaattg ccaaacaact atccgaaagg cgaaagcagc ccttcgattt caccggaagc
     2341 agatcttcgc tttgacaccc gattgtgggg agcggtcatt caggtgcttg gtctagtggg
     2401 ctatagcatc tatccgcagt cagtcaaatc accgatcgcc atgcaatatg attccctttt
     2461 tgtttaattt gcgtcgaacc gtaatacatc caagaatgcg ctgtattacc agtctcacca
     2521 cattgccgat taaaggaaac atcgtgaata ccctcatttc aaccttccag gaccaagatg
     2581 tcatctctcg acgttgagtt caatggcttc cagtttggaa cgctgacgca gctgatctgt
     2641 tcagcgggac cgataccagt cgcaacggtg agcagcatcg gttcagaccg gctgatcacc
     2701 cgcttcgagt ggtcgccggg cctactcgtt tcgaccgtgt cgggcgttcc cgtcgttgcg
     2761 ggcaccgtca ttgtccaggc gccggttcga atccaccacc tcagcgctgc cgagttgctc
     2821 gccaaccccg cagccgcacc cagcacaacc aatgcaacag cctgggttcg gtggagcgtc
     2881 gatcgctcgg gcctgctgac cggccacttc gtgcgcatcg atgcaaacgg gcgcggtgat
     2941 gtcttcagcc gtccctctga gatcggccgc cagcgattca ccgtaccgcg cgtcggggca
     3001 gccagcatcg caagcgcggc gctgctgtgg cgcgacgacg tcgtcaccat ccgcttttgc
     3061 acctcgatca ccgacgacgt gctcgctcaa ccgctgaaca gcatcgccga tctcggtatg
     3121 gcgtgggggg tgcgcatctc cgatggactg ctcgtcgaca tgctccggga acaactcacg
     3181 acgggattgg caaagatgcc accgggaacc gtcatcgagg acccgctgac ggtggtgtgg
     3241 aaacaggacg agcaaggtgt gtggggtgca agcgccgatg tcggcatcga gaagctcgac
     3301 gcctgccccg gactcttagg cgcggtcgac atctcggtgg cgttgtccac tcgccttacc
     3361 ttcagccctg acctcacccc gcaacggccg aagtttccac acggtcggct cgggctgcgt
     3421 gttgttgtcg agtccgacac gtccgattgg gattcgttcc ggtgctgggc tggtatcgca
     3481 ccgctggtag tcttcgcctc gcccctcttc agcggtgtcg gtgtgatagc gctgtttgtc
     3541 ggaagtttga tcgccgtctc tgccttcgtc gccggtaagg tgtccgacga aacagcgaac
     3601 gcgccgctgg gcagcgactt ccaacgcgag ccgagcggtg acgatgacgt gatcgcattt
     3661 gtcggagccg ttgagttacc ccaattcccc ggtcaggtcg acgttgccga agtccgctcc
     3721 agcgggctgg tctttgccgg cggcacgttc gtcgtcgtac cagcgctcca ccatacgact
     3781 ttcacaccag actgaggaac gctgccctcc ggctggatcg ggtcggtcga ttgccggtct
     3841 ggcctgtggt cggccagcgt cgacgtgcaa ccaatttacg tgaccgacca ggcgcagcta
     3901 ccagataacc cgccgggcac cggatacccg aagttgatgc cggtgaccat gttcccaacg
     3961 acgagcttct cgccgacgac gataactggc tggagtgtcg ccgcactgcg accgacgaac
     4021 cagcagatcg tgacggtggt tggcaagggc agcgagattg ttgggaaacc tgtcgtgttg
     4081 attctgcaca ccagcgccgg actgcgccgc tacgagattg cggccccgac cgacgtaccg
     4141 aagtccaatc agtttgaggt cgagttgcag cagatcgcgt gcaagcattg gggatttcgc
     4201 gaatgggcgc cccgaatgcc catgaagtgg agggtcgacc caccgccgta cgatttcggc
     4261 ttcaggccgt tgcgccagtg gatggtgaca ttcaagtcca ttccggaagg cgtcagcttc
     4321 gaacttcgta ccgttcacgg cgacgacatc gatgacggcc aggtcatcgt ccagcggacc
     4381 accgacgcga ccggcgagat gttcctcgac gccgtgaccg acgagcaagc cgaactgtcc
     4441 ctcaccaatg accttgagca ctcccctgca ggcgcaagcc tgcggcaacg gtggctgctt
     4501 cctaccgcgt cttccaagct agcggcccag ggcctttcgg tcatgcgtca cggcaatgcc
     4561 gttgtcgcac tcacaaccga cggcgcgacc gtgttgcgtt acaacacttc ctcagccaag
     4621 gggccgttgc tcgatctggt gaagtcgttc accgggatcg acggtgtgct gcggtcaact
     4681 cttgggcttg tcatttgggg ggcacgcggg ctgttcgtcg ttttggatga ggccaacaaa
     4741 atcgacttca aacgggtgac gaaggcaccg atccgggcgg ccgggcgggg cgccgatggg
     4801 gtggtgcgag tgatgcagga agatggcgtc atggaactgc gtgatgggtt tggcctcgaa
     4861 ccggcgaaca ccgaggtgct cggcttggtc gacgcgcaga gcacgtccag gccgctcccg
     4921 gacgggtcgg tggtagcgat cgatcccgac gcgacgattg ttctcgcccg gccgatgcgc
     4981 aggttctagt ctgagtccat gcgcgtttgg gtgcctccta accctgacat acttgccttc
     5041 atgtggttga tttccgttta tatactcccg accttatgga cattcaggcg cgtgcccttg
     5101 cggaaggtat tgcataaata tgcctcggat cgtttggtag aaaagccatc taacttaatt
     5161 actcgatcag gcgggcgctc cgataagcgc cgcaccagtt gggttttaat taaattaaga
     5221 tatgttacag ccgagttcgc agacattcgg caaacatctc atcaggaatc acattcatga
     5281 atattgattt cacgccacgc agtattactt gtcatcaaac cgtatccagt ccaagtgcgc
     5341 cttggggaat ggaggacggt tttcgtttgt attttgcggt agaggtcgct tttcaaagtc
     5401 cagatgtgat gatcagtggt cgcaaatacc cagagccgga tgttgtggac aattatccgg
     5461 ttaaagcggg gcaaaccatt agtttggaca ctttccctcc tgttgatcgt gcttcatggc
     5521 aaacaaaagt ttttaatgtc gatgaaggta atttggtcac tgtctggttg attggtgtga
     5581 atgagggctt gccgtttttt gggggtggcg gtggtggcag ccaattcagc accaaactgg
     5641 caagtaaagg cggacagttt ttgattaaaa aaattgcaga tcaagccagc gaggaagcgt
     5701 tgaagcgttc tattgcgggt gcgagtgaag gattgattaa acctgtgccg attgttggtg
     5761 cattggcaag tgcgctgatt gagttggcag aatatattga tgagtccaca gattgcagtg
     5821 gcagtgtgtt tatttataaa aaagaaatac tgggacgcga tttaattgcg cagctgttgc
     5881 agaatcgacg ctcaatcatg ctgagcttta atcaaaacca atcgctgcgc accgactcgc
     5941 cggataatcc ttgcctgaca ccgtcttatc aagcaacgct tgatattaat ttgcgctcgg
     6001 tattaaaagt aacccccagc ctaactttgt taaaaaccat caccactgca ccggttgaat
     6061 ttaacccact atttatttct tgcgcaaaaa aaaccagtga gcctattcag gtgtggagtg
     6121 aagtcaggga ttttaaaatt gaatttattc ccaactttac ttttcagctg atgccaatta
     6181 tctggaaaat tgagaaccag gttctaaccc agcagcaatc gaccggcacc tttaatttgc
     6241 cagtgacaat acagccgaat cagcaaaaca aactgtctga tgtcaatatt cgatacgaaa
     6301 tcgatttacg gcagcatcta attctgagca ccacaggtag tgagggcagc tttcagattc
     6361 gtgtgcaagc gtttttacca ctgcaaagtc agcaagcccc gtttttaatc tatcaacata
     6421 ttttggtggt ggatagccag gacatgttgg gtaataaagg ctatcaagaa tatttggatt
     6481 gcatgggtgc caagctgaat ggcttgatta ccgaaattga taaaacccaa tttgttaggc
     6541 aacgcgaatt tttaccacgt caacccttca agcagctagt gacgtcacgc agtgagcgtg
     6601 ctcaaaaaca aattgaagcc atgggcctag atgatcgtat cagtgtgtcg cagacggtaa
     6661 acgttcggga aatggaagaa ctgctgggtt ttgcgatcaa gctgtaaaaa aagctattct
     6721 atgtattgaa ttacctgccg tccgtgggct accgctcccg ccgcagtgcc gaccggcacg
     6781 gacaaaaaac tgattgtcgc ccgcgaatgc ctgcttcgga gtttttgcga tagccaaata
     6841 gcaaaattgg accggcacgg gctatgaccg ctttccagaa aatggttcaa cttcatcttg
     6901 tccggcgacc agcagatccc gctgcagtgc cgaccgccag tttgaaatga tgatttttgc
     6961 cgctgaatga ccgctcctga gattcgggtg tcaaaaacga aagtctgctt gcggcgaaat
     7021 cgaaggtcag cttctgactt ttcgttgggg ttcatgctgg cctggtgtac gtcgggtttg
     7081 ggtctgttct tgacggtgat ggttgtgccg acggttttcc agatcaatac cggctgaaga
     7141 cacttatgta tagctatgca tacgggtctg ttcctgtcag tgatgccatg gacaacccga
     7201 tttccaggcc aatgccggct acaggctgtc aggagtcggt cacgagtgcc cggtatctgg
     7261 aggtccaatt tcacaatttc tgtggccgag aagccgccgc gtgtggtggg cttttacgtg
     7321 cccaaagctg tctccccagt ggccagagga ttcacgccaa tagccacctg tactactcga
     7381 atgcgtttag tcgtcacgga tgtatccagc acctgttccg ttggtcaaaa cggaatcctt
     7441 cactcgccac cgcagttcgg taggccatcg cgacggtgtg gccaacagtg actccgtatg
     7501 aatgattgtc tcctcatgca cttcagcgtc aaatcgctcc acgactctgt atcgcttgag
     7561 ggttccgtcg gatccatcta agaccgcata gctgactcgg tgtcgacaac cgagtctgcc
     7621 actcttagcc gcgtggtgcg gactacttac ccattcatgg tcgcttcaat cgcggaagta
     7681 gacgttagcc aatgcgttca aaaattgctg ctcgttgtgc gccgctacat caatgtcatt
     7741 tcgcacttca gaccacccct tttcgtgcgg aacgctgtga acgttttcg
//
LOCUS       DAOSLL010000153         7732 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8694_length_7732_cov_4.731275, whole genome shotgun sequence.
ACCESSION   DAOSLL010000153 DAOSLL010000000
VERSION     DAOSLL010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7732
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8694_length_7732_cov_4.731275"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            466..993
                     /locus_tag="PLB25_08500"
     CDS             466..993
                     /locus_tag="PLB25_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41652.1"
                     /translation="MEVIDRIRAEQLLLTLDLKIGDWNELTVRSPSKATHRSFAAPTS
                     ALELYVAAHRLASWISSGSWTLLQIDNSTSPMDDEVAVFENLVLKGQHSWDIAAQHTF
                     LLDGSARQSTLVLLIYFALLFTWHIHLVSEASSSGQRLALQDGVVYFFGDASTTAAAD
                     ELIASLISEPRMIAS"
     gene            1129..1380
                     /locus_tag="PLB25_08505"
     CDS             1129..1380
                     /locus_tag="PLB25_08505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41653.1"
                     /translation="MTIIPAVLGALATALGIWCAVLGLSAIKHLREADQIDKAIGWSL
                     WWCFDVDRYSKEGRKLCKLGQLLAFAAVVLWVCVYTIER"
     gene            1764..2447
                     /locus_tag="PLB25_08510"
     CDS             1764..2447
                     /locus_tag="PLB25_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41654.1"
                     /translation="MKMPPASLNSRRPRRHASRRCSASQFSRTHLTQTRQVLNPDASA
                     NAQAVVHYTSYIYNAAGQFTRLIYPSGRTLSISYANGLPSSLGLAQNAVAGAVALMTD
                     LQYAPMGPLKRWNWAMSSGPLAHDRVFDTSGRLVRYPLGEHLRAKGARLRLNPKLWVS
                     VASVFDRCQRPVQCACCNTESSTYSTIFCCSLGSALIRSRGRCSVGVGPRLRASGDGG
                     SQFVTTAAF"
     gene            2810..4078
                     /locus_tag="PLB25_08515"
     CDS             2810..4078
                     /locus_tag="PLB25_08515"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOE41655.1"
                     /translation="MTIEHAKLPVDRHANQRLTSDMALLLAISQDFVGAPAMGETLNN
                     AVEQIMAYMDTEAASIFLLDASGERLTCLACAGPVSIVGMSIAHNQGIIGRALTGNCV
                     QMVRDVSADPDFSSVVDESTGFTTRSILCAPLVVKGRMLGTLEVINKRRGDGLFDLDD
                     KQFLQVLAGMVSLAVHNARMTEALVEQERMRRELDLAREIQRSLLPDQPAADFPVQGL
                     NLSAMEVSGDFYDFFPLPDGRIVWGLGDVSGKGMNAAMLMAKTISLFRCLGKTVRDSA
                     DLLAMLNDEIVETASHGMFVTMAAGVFDPASGEVVFANAGHQPPLLRSLDGTYCELED
                     SSLPLGILPGVQYTRQRAQLGGGRLFIFTDGVTEGRTHGNQMLGLPGLQTLLDQHAAL
                     APAEQLARVAAALVRPGMRLHDDLTMLVIG"
     gene            4078..4488
                     /locus_tag="PLB25_08520"
     CDS             4078..4488
                     /locus_tag="PLB25_08520"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE41656.1"
                     /translation="MQALLDQTFAASSRALAAVRAALREACQQAGCGEECAEQCVLAV
                     NEACMNVIQHGYQFADGQHFTLRLGIADGVLQAQLLDNGVLATDADLCPRALDELRPG
                     GLGVLFMRELMDQVGYQLAPQGFTNCLQLTKRIY"
     gene            4499..4828
                     /locus_tag="PLB25_08525"
     CDS             4499..4828
                     /locus_tag="PLB25_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOE41657.1"
                     /translation="MEIQIEDWPPYQLVRLGGDVDLHSSPRAREAILDCLKQKMALLV
                     DLSAVAYMDSSGVASLVEGYQTAKKHGLEFGLVAVAPSAMKVLKLARLDKVFPMHATL
                     PERRAGV"
     gene            4794..5621
                     /locus_tag="PLB25_08530"
     CDS             4794..5621
                     /locus_tag="PLB25_08530"
                     /inference="COORDINATES: protein motif:HMM:NF014460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE41658.1"
                     /translation="MPRCRSVGRVFDPLRSLVASVGQRSLLAVREVGYHAALLVEACY
                     WLMFGRLRRQPVRLGAIFKEAVGVGVNAIPIVSLASLAVGMMLAIQGIYTLRTFGAEG
                     QVVLGISLSVTREFAPLITGIFVAGRSGSAIAARIGTMQISQEIDALRVIGINPVRYL
                     VAPLLAAMLLTLPALTILSDLIALFGGALYCAGELSLPIDIFFQRSFEVLIAEDVLHG
                     VLKSFIFAVIITLIGAANGFNVSAGAEGVGQATTRSVVLGCIGIILADMVFTFLMSR"
     gene            5622..6422
                     /locus_tag="PLB25_08535"
     CDS             5622..6422
                     /locus_tag="PLB25_08535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE41659.1"
                     /translation="MQSPELAIDVTDLVTHYGARKILHGISFQARQGEIMVIMGGSGS
                     GKSTLLRHLLGLHQPTSGSIKLLGRDITKLSADEMYDLRKNMGVAFQGGALFNSMTVG
                     ENVELPLREHTRLDDNTIRIMSRMKLEVVNLAGFENLMPAELSGGMIKRAALARAIVM
                     DPRLLFFDEPSAGLDPVVSAELDELILLLRDAMNMTIVVVTHELDSAFKIADRITVLD
                     QGRILMSDTTAAVRASDNERIQSLLNRTPRNEVINPDEYLERLTREIF"
     gene            6450..7334
                     /locus_tag="PLB25_08540"
     CDS             6450..7334
                     /locus_tag="PLB25_08540"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOE41660.1"
                     /translation="MKRDNVNYLLVGSFVLLMAAVLLYALYRITGHRAKGDIYFTHFP
                     NVTGIKQGTVVTYEGFEVGNVAEVDPVLRNQHTAYRVTLNMRRPVRIPVDSRAVIATP
                     GMLSAPLVEIKEGNDQHILAAGGDIPGSSNPSMMESIGTLAADLGRLTETSIKPLLAQ
                     LNQNMTPAMADMRSTLERINRSAGSINALFSEDNVRHWNRMLANGDGASVNVLKLSTD
                     LLGVRTEVEGLVKDSRGIVNSSGNNLKDSLRRIDAALYQLEATGRNLNEFSRHLRHNP
                     GALIQSRPPTDAASGAAQ"
     gene            7331..>7732
                     /locus_tag="PLB25_08545"
     CDS             7331..>7732
                     /locus_tag="PLB25_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41661.1"
                     /translation="MSRAINQIRFTLLGFCLLGLCLLLVACGNLPPAPTDHFYRLQPV
                     NLPPVAHKLPAAFAVQTFRADSLYAERPIIFTEQASLRQLRQYHYHLWLYPPAQMVQE
                     HLVASLGNTIAWRGGSQAPDVLQGRILRFERV"
BASE COUNT         1501 a         2265 c         2349 g         1617 t
ORIGIN      
        1 gagtgatccg attgggttgg cgggggggat tgatacttat gggtatgtgg gggggaatcc
       61 gctgagttat gtcgatccag agggtttagt tatgatcccg aacccgaatg gtgttgtccc
      121 tggcggtccc tggactcctc atgatgcgaa tcggtcgggg cagtttttgg gcaccaagcc
      181 agatggcggt ggaggacgcg ctcagtgtca gttcgttccc gatgaggcaa atggggggcc
      241 gcccggtaac aagggctact ggaagacgaa tcagccgggc caaaagggtt ggcaacggta
      301 cggcctggat ggcaagccaa tcgacgcagg tgaagcccac aagatccctc tgccgcgtat
      361 tccaggattg gcccctttgc tctgcccact ttgtacgatc atttatccga acggtcctgg
      421 tgggtatctc tctggcccag gtccgtctta attgttgagg gtgcgatgga agttattgat
      481 cgaattcggg cagagcaact tctgctgacc ttggatttga agatcggcga ctggaacgaa
      541 ctcaccgtcc ggtcaccttc gaaagctaca cataggtcat ttgcggcacc taccagcgca
      601 cttgagttgt acgtcgccgc gcaccgactt gcgagttgga tcagttcggg tagttggacc
      661 ttgttgcaga ttgataactc gacatcgccg atggacgacg aggtcgccgt cttcgagaac
      721 cttgttttga agggacagca ctcgtgggac atagccgcgc agcacacgtt cttgctggac
      781 ggaagcgcgc ggcaatcaac gctggttctg ctgatttact tcgcgctgct cttcacatgg
      841 cacatccatc ttgtgtctga agcatcgtca agtgggcaac gtcttgccct tcaagatggt
      901 gtggtctact tttttggaga cgcgtccacg actgctgcgg ctgacgagtt aatagccagc
      961 ttgatcagtg aacccaggat gatcgcctct tgaagtggcc acacccgtgg tcaccctgcc
     1021 gacgtggcac tttacttggg gttgtcctct gggagaaaag gggccaggct cagattgaat
     1081 cctttgacgc catcgtttga aggcgctacg aaaccaaggg gggtcgccat gacaatcatt
     1141 cctgccgttc taggtgcctt ggcaacggcg ctaggaatct ggtgtgcggt acttggcttg
     1201 tccgcgataa agcatttgcg tgaagccgat caaattgaca aggctatcgg gtggtcgttg
     1261 tggtggtgtt ttgatgtaga tcgttacagc aaagaagggc gcaagctgtg caagttgggc
     1321 cagttgctgg cctttgctgc cgttgtgctc tgggtttgcg tgtacacaat cgagcgatag
     1381 caaagtcgtt ggaatagatg tgcaagacgg cctagatcgg ggtaaatcgg tggcatccac
     1441 cgcacaccca tcggcaccaa cgcccgattt cctctctctc aatctaaaca aaaaaaccac
     1501 gtgccaaccc ttcgctgcag agaactgggg atcacaggcg gcgcatgtcc gcgagggtgt
     1561 tgcgtttcaa gcagatcagc cctgcacctt gctcctgagc tggtcgcgct caatgcaggc
     1621 atcaatcaat tgcaacgcct ggcgattctc ggtcgctccc agtggaaaat gggtcaggct
     1681 cggattagga aattgacatc acatttactt caaaatagat agctaccagc gcacgattca
     1741 caagggctaa agcccagttt gacatgaaaa tgccgcctgc atcactgaac tcccgccgac
     1801 cccgaagaca tgcatctcga cgttgcagtg ccagccagtt cagccgtacc cacctgaccc
     1861 aaacccgcca ggtcctcaac cccgatgcca gcgccaatgc gcaagccgtg gttcactaca
     1921 ccagctacat ctacaacgcc gcaggccagt tcacccgcct gatctacccc tccgggcgta
     1981 cgctgagcat cagctacgcc aacggcctgc ccagcagcct ggggctggcc cagaatgccg
     2041 tcgccggggc cgtcgccctg atgaccgatc tgcaatacgc cccgatgggg ccgctcaagc
     2101 gttggaactg ggccatgagc agcggcccgc tggcccacga ccgggtgttt gacaccagtg
     2161 gtcgcctggt ccgctacccg ctgggtgaac acctgagagc aaaaggggcc aggctcagat
     2221 taaatccaaa gctgtgggtt tcggtggctt ctgtttttga ccgctgccaa cgccccgttc
     2281 aatgcgcctg ctgcaacacc gaatcaagca cctacagcac gatcttctgc tgctccttgg
     2341 gcagcgcgct gatccgttcc aggggccgct gtagcgtcgg cgttggcccg cgcttgcgtg
     2401 catccggcga tggtggctcg caattcgtga ccaccgccgc cttctaaacg acgttgatct
     2461 gaacaagaaa gtctgtcgaa aagcgctccc agttgcgttt gacctgttcg tcaaacccca
     2521 atcaaagcgc gttaatgcgc ccaggccgat ggcctgactg ttcccgcgcg cagacccgac
     2581 accaagcgca atttttcacc agacagtctg gcaaactgtg gtggaatcaa aaccagcccg
     2641 aatgatggca aggcaacact tgccactaat tcctgtctgg cagcagtaca cagccagtgt
     2701 gtggcgaaag gcaagtcagg cgcgactgaa ttcaattgga tgaattccgt cggcttgcgc
     2761 cctggccacc tgacggcacg atctttcacg tttaccggag ctactcgttg tgaccatcga
     2821 acacgccaaa ctacccgttg acagacatgc caatcaacgg ctgacgagtg acatggcgtt
     2881 gctgctggcc atcagccagg actttgtcgg agccccggcg atgggtgaaa cactgaataa
     2941 tgccgttgag cagatcatgg cctacatgga caccgaggcg gcttcgattt ttttgctcga
     3001 tgcctcgggc gagcggttga cctgcctggc ttgtgccgga ccggtctcca tcgtcggcat
     3061 gagcattgcc cacaatcagg gcatcattgg gcgcgcgctg actggcaatt gcgtgcagat
     3121 ggtgcgcgac gtgtccgccg accccgactt cagttcggtc gtcgatgaaa gcaccggctt
     3181 taccacccgc tcgattttgt gcgcgccgct ggtggtcaag gggcgcatgc tgggcacgct
     3241 ggaggtcatc aacaagcgcc gtggcgacgg gctttttgat ctggacgaca agcagtttct
     3301 gcaagtgctg gcgggcatgg tttcgctggc tgtgcacaac gccagaatga ccgaggcgct
     3361 ggttgagcag gagcgcatgc ggcgcgaact cgatctggcg cgcgaaatcc agcgcagtct
     3421 gttgccagat cagccagccg ctgactttcc ggtgcagggc ttgaatttgt cggcgatgga
     3481 agtttctggc gacttttacg attttttccc attgcccgac gggcgcatcg tctgggggct
     3541 gggcgacgtt tcgggcaagg gcatgaatgc ggcaatgctg atggccaaga ccatcagcct
     3601 gtttcgctgc ctgggcaaga cggtgcgtga ctctgctgac ttgctggcca tgctgaacga
     3661 cgaaattgtc gaaaccgcca gccacggcat gttcgtcacg atggcagcag gcgtgtttga
     3721 cccggccagt ggtgaagtgg tgtttgccaa cgccgggcat cagccgccgc tgcttcgcag
     3781 cctggacggc acctattgcg agctggagga ttcgtcgctg ccgcttggca tcctgccggg
     3841 cgtgcaatac accaggcagc gtgcgcaact cggtggcggc cgactgttca tctttaccga
     3901 cggcgttacc gaagggcgca cccacggcaa ccagatgctg gggctgccag ggctgcaaac
     3961 cttgcttgac cagcacgcgg cgctggcacc ggcagagcaa ctggcgcgtg ttgccgcagc
     4021 gctggttcgc cccggcatgc gcctgcatga cgatctgacc atgctggtga tcggctgatg
     4081 caggcgctgc tcgatcagac ctttgcggcc agcagccggg cactggcggc ggtgcgcgcg
     4141 gcgctgcgcg aagcctgcca gcaggccggt tgtggcgagg agtgcgccga gcagtgtgtg
     4201 ctggccgtca atgaggcctg catgaatgtc atccagcatg gttaccagtt tgccgacggg
     4261 cagcatttca ccctgcgcct gggcatcgct gacggggttt tgcaggcaca gttgctcgat
     4321 aatggcgtgc tcgcaacaga cgcggatttg tgcccgaggg cactcgacga gcttcgccct
     4381 ggcgggctgg gcgtgctgtt catgcgcgag ctgatggacc aggtgggcta ccaactggca
     4441 ccgcaaggtt tcaccaactg cctgcaattg accaagcgca tttattgagg aaaaccaagt
     4501 ggaaattcag atcgaagact ggccaccgta ccagcttgtg cgccttggcg gcgatgttga
     4561 cctgcattcc tcgcccaggg cgcgcgaggc cattctggat tgcctcaagc aaaagatggc
     4621 gctgctggtc gatctgtcgg cggttgccta tatggacagc tcgggcgtgg cgagtctggt
     4681 cgagggctat cagacggcaa aaaaacacgg ccttgaattt ggcctggtcg cggtagcgcc
     4741 gtcggcaatg aaggtgctca agctggcccg acttgacaag gtgtttccga tgcatgccac
     4801 gctgccggag cgtcgggcgg gtgtttgatc cgctgcgctc actggttgca agcgtcggcc
     4861 agcgcagctt gctggcggtg cgcgaggtcg gctaccacgc ggcgctgctg gttgaggcct
     4921 gttactggct gatgttcggg cgcttgcgcc gccagccggt gcgcctgggc gcgatcttca
     4981 aggaggcggt tggtgtcggt gtcaacgcca ttccgattgt ctcgctggct tcactggcgg
     5041 ttggcatgat gctggccatt cagggcattt acacgctgcg cacttttggc gcggaaggtc
     5101 aggtggtgct gggcatttcg ctgtcggtga cacgtgaatt tgcgcctttg attaccggca
     5161 tcttcgttgc cgggcgctcc ggttcggcca ttgccgcgcg catcggcacc atgcagattt
     5221 cacaggaaat cgacgcgctg cgggtgatcg gcatcaaccc ggtgcgctat ctggtggctc
     5281 ccttgctggc ggccatgctg ctgaccttgc cggcgctgac catcctgtcg gacctgatcg
     5341 cgctgtttgg cggggcgctg tattgcgctg gcgaactgag cttgccgatc gacatttttt
     5401 tccagcgcag ctttgaagtg ctgatcgccg aagacgtgct gcacggggtg ctgaaaagct
     5461 tcatttttgc cgtgatcatc accctgattg gtgctgccaa cggttttaac gtgagcgccg
     5521 gagctgaagg cgtggggcag gcgaccaccc gctcggtggt gctcggctgc attggcatca
     5581 tcctggccga catggttttc acttttttga tgagccgctg aatgcagtcg cctgaactgg
     5641 ccattgacgt gacggacctg gtgacccatt acggtgcgcg caagattctc catggcatca
     5701 gctttcaggc gcgccagggc gagatcatgg tcatcatggg cggcagcggt tctggcaaaa
     5761 gcacactgct gcgccatctg cttggcctgc atcagccgac ctcgggcagc atcaagctgc
     5821 tgggccggga catcaccaag ctcagcgccg acgagatgta cgacctgcgc aagaacatgg
     5881 gcgtggcctt tcagggcggg gcgctgttca attcgatgac ggttggtgaa aacgtcgagc
     5941 tgccgctgcg cgaacatacc cggctcgacg acaacaccat ccgcatcatg agccgcatga
     6001 agctcgaagt ggtcaatctg gccggctttg agaacctgat gcccgccgaa ctatcgggcg
     6061 gcatgatcaa gcgcgcagcg ctggcccgcg cgattgtgat ggacccgcgc ctgctgtttt
     6121 ttgacgagcc ttcagccggg cttgatccgg tggtatcagc cgaactcgac gagctgatct
     6181 tgctgctgcg cgatgcgatg aacatgacga ttgtggtggt cacgcacgag ctggacagcg
     6241 ccttcaagat cgctgaccgc atcacggtgc tcgatcaggg gcgcattttg atgtcagaca
     6301 cgacagcagc agtgcgcgcc agcgacaatg agcgcatcca gtccctgctc aaccgcacgc
     6361 cgagaaacga agtcatcaac cccgacgagt acctggaacg gctgacccgc gagattttct
     6421 gaccatgagg ctttcaggat gaccacacga tgaagcgcga caacgtcaat tacctgctgg
     6481 tcggcagctt tgtgctgctg atggccgccg tgctgctgta cgcgctgtac cggattaccg
     6541 gacaccgcgc caagggcgac atttacttca cccatttccc caacgtgacc ggcatcaagc
     6601 agggcacggt ggtgacctac gagggctttg aggttggcaa cgtggccgag gtcgatccgg
     6661 tgctgcgcaa ccagcacacc gcttatcgcg tgacgctcaa catgcgtcgg ccggtgcgga
     6721 ttccggttga cagccgcgcc gtcatcgcca cgccaggcat gctcagcgca ccgctggtcg
     6781 agatcaagga gggcaatgac cagcacattc tggcagcagg cggtgacatt cccggcagca
     6841 gcaacccgag catgatggag tcaatcggta cgctggcagc cgatcttggc cggttgaccg
     6901 aaaccagcat caagccgctg ctggcccagc ttaaccagaa catgacaccg gcgatggccg
     6961 acatgcgctc gaccctggag cgcatcaacc gcagcgctgg cagcatcaat gccttgttca
     7021 gcgaagacaa cgtcaggcac tggaaccgca tgctggccaa tggcgacggc gcgtctgtca
     7081 acgtgctcaa gctgtcaacc gatcttttgg gagtgcgcac cgaggtcgaa gggctggtca
     7141 aggattcacg cggcatcgtc aacagcagcg gcaacaacct gaaggactcg ctgcgcagaa
     7201 ttgacgcggc gctctaccag cttgaagcca ccgggcgcaa cctcaatgaa ttttcacgcc
     7261 atcttcggca caatccaggt gcgctgatcc agagccgtcc gccaaccgat gctgcctcag
     7321 gagccgccca atgagccgag caatcaacca gatacgcttc accctgctgg gcttttgcct
     7381 gctgggcttg tgcctgctgc tggtggcctg cggcaacctg ccaccggcac caaccgacca
     7441 tttctaccgt ctgcaaccag tcaacctgcc gcccgtggcg cataagctgc ccgctgcatt
     7501 tgcggtgcag actttccgcg ccgacagcct gtatgccgag cgcccgatca tcttcaccga
     7561 acaggccagc ctgcgtcagt tgcgccagta ccactatcac ctgtggctct atccgccagc
     7621 acagatggtt caggaacatc tcgttgccag cctgggcaac acaattgctt ggcgcggtgg
     7681 cagtcaggcg cccgatgtgc tgcaaggtcg catcctcagg tttgagcggg tc
//
LOCUS       DAOSLL010000154         7712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8724_length_7712_cov_3.796526, whole genome shotgun sequence.
ACCESSION   DAOSLL010000154 DAOSLL010000000
VERSION     DAOSLL010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7712
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8724_length_7712_cov_3.796526"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..697)
                     /locus_tag="PLB25_08550"
     CDS             complement(128..697)
                     /locus_tag="PLB25_08550"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOE41662.1"
                     /translation="MTRKRPALRARIRFILRFIESVGLRSAELLNATLEDLRLESEGW
                     VMQVHGKGAKNRIAAVPRQAFDALQQYLAFRGVGPVQSAPPKAPLLASTLDPMESIGC
                     QALYEQVKGRLYKAIAASALPFNIRMKRSGASTHWLRHTYGTGSIAREVPLDVIQAQI
                     GHGSIQTTTAIFGRAPIRRRVDEFGKAFG"
     gene            complement(831..1004)
                     /locus_tag="PLB25_08555"
     CDS             complement(831..1004)
                     /locus_tag="PLB25_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41663.1"
                     /translation="MNFDDCGNFLALLQNILRQWISRIHTPPGAPGWAPFGSPLSRKS
                     QAQAIVIVESLFT"
     gene            complement(1017..1199)
                     /locus_tag="PLB25_08560"
     CDS             complement(1017..1199)
                     /locus_tag="PLB25_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41664.1"
                     /translation="MTHLPRPALPAVADSMRMASGDHEAIDSWIGARAGSELIATVYR
                     REAMGLLLWLQYECQG"
     gene            complement(2291..4396)
                     /locus_tag="PLB25_08565"
     CDS             complement(2291..4396)
                     /locus_tag="PLB25_08565"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HOE41665.1"
                     /translation="MTAIAPDGRDSFEPLFHSRPARKSGRPLGSRAQVVQDARSLGIH
                     HFAFLRCSLLGLDLREAFDRYLAWSETSSDLRYIHNRREGLLKHVIDAARHLDATLPE
                     SSKITPLIDLLRSDATLKPAVVLPSLQDWVQEQGFDPDAWSEADLLIEYKAHFGLDMG
                     AFLESASSLKDPAGERVHALNHLAMLLSVAPAATDRLESWFARPVTKCLRNVGLLSLG
                     DLVRFVNVYGYRWHARIKGFGVQRADQVVAWLQMQAEHLNMGIAQSTLEPKSRRLLRQ
                     NAQGQLVAARVISGLGQFGQGSLVQMSLERIQGSAVLSGSSGEFRSHMANTLGASNDF
                     EAVNAWLAYYAEKPSTQRSYRKEIERFLLWCTQQLNKPLSSVTSPDCLKYREFLAAVP
                     ATFIQSAPVQRTDPSWRAFRGQPSAASQKQALVIIQTMFGALCDAGYLVANPMRALMK
                     SFDLPASKVDIRRSFTEAEWAHVLRCLQDIPAGPERARLQCILELLVSSGIRLDELAK
                     ARHSHLRKESLPDLPQTWVLTVTGKRAKTREVPLNDEVVLLLAAHGKEFMKDDVDCTD
                     ADNLPLIRTLHPSVAQWGMSESGALVSTAVSGKPGSALSGAGIYAVLKRFFAMASKRA
                     EQAGLDALRFEKASTHWMRHTFVRQALVDGVPIEVVSELAGHASIDTTSIYSTQELAR
                     KIRAIKVMKRRVAAWASSR"
     gene            5091..5468
                     /locus_tag="PLB25_08570"
     CDS             5091..5468
                     /locus_tag="PLB25_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41666.1"
                     /translation="MFKDVLIGRNLKGVTDREDFSLENWPRPDAGAMSPTDLALLLKR
                     KEAVERYLQATSSATIYTATRLAPRRQFILLGPPLFRQGHFYEKPGNVFMQMDVGDFL
                     TTELIQLILRHDYKDNHDAFGTD"
     gene            5687..5986
                     /locus_tag="PLB25_08575"
     CDS             5687..5986
                     /locus_tag="PLB25_08575"
                     /inference="COORDINATES: protein motif:HMM:NF027059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsR family
                     toxin"
                     /protein_id="HOE41667.1"
                     /translation="MEKNTPSYSLADILAQMQSVADMNLTISARTGIRDSGMGQMDAL
                     AVVQSLTRKHFFKSMTTHADYRVWQDVYHGQWGDLSLYIKFQRASEYFVVSFKEL"
     gene            5988..6392
                     /locus_tag="PLB25_08580"
     CDS             5988..6392
                     /locus_tag="PLB25_08580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system MqsA family
                     antitoxin"
                     /protein_id="HOE41668.1"
                     /translation="MASKWNDQPCHLCGAGTLHDGVKLVEQAYKGKIFKSESRGAFCD
                     HCADGFVEFDQDEESAWLAFRNQVDAAEVADLARIRKKLKLSQLEAARLAGGGKNAFS
                     RYERGQAKPVAAVINLFRLLDGHPELLSELRT"
     gene            6532..6764
                     /locus_tag="PLB25_08585"
                     /pseudo
     CDS             6532..6764
                     /locus_tag="PLB25_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011880201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(7118..7426)
                     /locus_tag="PLB25_08590"
     CDS             complement(7118..7426)
                     /locus_tag="PLB25_08590"
                     /inference="COORDINATES: protein motif:HMM:NF016255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HOE41669.1"
                     /translation="MAEIIEIDSVACLKPLAVGIHKTIIATLQAQPEFQDCSRTTLHQ
                     MLAKVLNGHTSRPEYLAGVLKFAQRYGINGESNGEVTEKHKADASKRTCSVLPFGSSD
                     "
     gene            7425..>7712
                     /locus_tag="PLB25_08595"
     CDS             7425..>7712
                     /locus_tag="PLB25_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41670.1"
                     /translation="MIGLLAKYAPTIGVSFSICSLLRADSSASCWMFSCFLSSFSAFG
                     IGLSFFEDSGALAGTAAGFGTGTGVTAGAIADFLRVSLGRSVIIFVPLMFGF"
BASE COUNT         1743 a         2070 c         2113 g         1786 t
ORIGIN      
        1 ggatgcctgc tgcccgagac tgttggcaaa gatagacaac atgttggcaa gattgatgcg
       61 catagcgcct aaactcacca gaatcggata gatttttgtc tgtttagccg ccatcaactg
      121 agcagcttca accaaacgcc ttaccaaatt catccacccg tctccgaatg ggtgcacgcc
      181 caaaaatggc cgtggtggtc tggatactgc catgcccgat ttgcgcctga atcacatcca
      241 gcggtacttc tcgggcaatc gaaccggtgc cataggtgtg acgcaaccag tgtgtcgatg
      301 cgccggagcg tttcatgcga atgttgaagg gcagggcgct ggcagcaatc gccttgtaca
      361 gccgaccctt gacctgttcg tacagcgcct ggcagccaat ggactccatc ggatcgaggg
      421 tgctggccag tagcggtgcc ttgggtggtg cgctttgcac cgggccaaca cctcgaaagg
      481 ccagatattg ttgcagtgca tcgaatgcct gacgaggcac ggcggcaatg cgatttttgg
      541 cacctttgcc atgaacctgc atcacccagc cttcggattc aagccgcaag tcctctaatg
      601 tggcgttgag caactcagcc gagcgtagcc ccactgactc gatgaaacgc aaaatgaagc
      661 ggatgcgcgc acgcagggca gggcgcttgc gcgtcaatga acttcaggac ttacagcatg
      721 acggcttcac tgaaggcttt gctgtcgagt atcttctcat tggcatcatc gccggtcttg
      781 cggtttacca atagccatgg attggccggt aggtagcgtg ccgcctgtag tcaggtaaac
      841 agcgattcga cgatcacaat cgcttgcgcc tgactcttgc gactcaaagg acttccaaaa
      901 ggtgcccaac ccggcgcacc cggtggggta tggatgcgcg aaatccactg gcgcaggata
      961 ttctgcaata acgccaggaa gttgccgcag tcatcaaaat tcatttgggt gagcgcttag
     1021 ccctggcatt cgtattgcag ccagagcaac agacccatcg cctcacgccg atacaccgtg
     1081 gcgatgagct cagaccctgc gcgtgcgcca atccaggaat cgatggcctc atggtcgccg
     1141 ctggccattc gcatcgaatc tgccacagcg ggtagggcgg ggcggggcaa atgggtcatc
     1201 ggttcaggca agtccactgc tctactaagg gcgaacagtc cgtgattggt gaccaaagca
     1261 aacgtcgttt ttaccctgca agcatctgcg gcagcagcat agccaagtgg cttttgatgc
     1321 gttgcgcttc ggttttgcca acgccgggca ggctgctgta ccagatacct ccaatagata
     1381 tgcttttgat caacgagtac aggacttgtg attgtcgcaa tcaagggcca ggcgtgatct
     1441 ccgggaccgc cgacggcgat ctgctaacca agatgactga gagcgagcat gacggcgcag
     1501 atcagcgcaa atgatgtcag accatcgatc cagccagcgg tcagtttagt gaatcgtgac
     1561 atttactact tgccgctcaa cctgcgcaag caacggcagc tatttatcgc gcaattgttt
     1621 ctgcgaggac ctattcgata actggtaggg cgactatcac ctcgggccat atccgtacaa
     1681 ggtcagatat gatccgtccc tccagatttc actaaacaag aagtatttcc ataatgtgaa
     1741 aatgtggcat caacagccac ttgcctcaga ttgacgccgg ggccaattga tcgccaagcc
     1801 tttcccgctc tgataccagc ttgccaaatt cctcctccgt atgccgatgt tccgtcgtgc
     1861 cagcatcgcc atgctccaag ctgtgatgac tcttggcacg ggtctgaagt tcgagtcttc
     1921 accgatgctc agcaggtgcg cgccaaatgg ccgcttgacc ttgttgatct gacctcgcag
     1981 cccaaagcac gccattgagc tcattcttcg cacaaccgct cctaaaaccg acctatcaga
     2041 acgctccgta tgctctcaaa gctgaccagg caaagggcag ggtgcttggg ggtgtaaaaa
     2101 tcgcttggag ggcgtttaaa tcggtcaatt cggctgattt ttccactgca ggtgatcact
     2161 gtgcctgagt tgccgaaggt gatagtcggc atcccagcgc agcgagcgat ctcgtgtcgt
     2221 tgaccgacgc attgagtgtc acccgccaag ggtgagatcg ggcaggcgat gaactgcgtt
     2281 ggcaagtgca tcaacgcgat gaagcccagg ccgcaaccct gcgcttcatt accttgatcg
     2341 ccctgatctt cctggcgagt tcctgcgtgg aataaatgga tgtcgtgtca atcgaggcat
     2401 gccctgccaa ttcagacacc acttcgatcg gcaccccatc gacgagcgcc tgtcgcacaa
     2461 aggtgtggcg catccaatgc gtgctcgcct tctcaaagcg caacgcatca agtccggctt
     2521 gctccgcccg cttggatgcc atcgcaaaga agcgtttgag tacggcataa atgccagcac
     2581 cagacaaagc actgccgggc tttccagaaa cagcagtgct caccagcgca ccgctctcag
     2641 acatgcccca ctgcgccact gaggggtgca gggtgcggat cagcggcaaa ttgtccgcat
     2701 cggtgcagtc aacatcgtct ttcatgaact ccttgccatg ggccgcgagc agcaggacta
     2761 cctcatcatt gagtggtact tcacgggtct tggcgcgctt gcctgtcacg gtaaggaccc
     2821 aggtttgcgg caagtcgggc aagctctcct tgcgcagatg gctgtgacgc gcttttgcca
     2881 gctcgtccag acgaatgcct gaactcacca acaactccag aatgcattgc aaccgggcgc
     2941 gctcagggcc tgccgggata tcttgcaaac accgcaggac atgagcccac tcggcctcgg
     3001 taaatgatct gcggatatcg accttgctcg ctggcagatc aaagcttttc atcaaggccc
     3061 gcatcgggtt ggccaccaga taaccggcat cacacagcgc accaaacatg gtctggatga
     3121 tgaccaacgc ctgcttctgg gaggccgccg agggctgccc cctgaaggct cgccacgatg
     3181 gatcagttct ttgaaccggt gctgactgga taaaggttgc cggcaccgca gccagaaact
     3241 cccggtactt caggcagtcc ggcgatgtaa ccgaggacag gggcttgttg agctgctgcg
     3301 tgcaccacag caaaaaacgc tcaatctctt ttctgtagga gcgctgggtg gagggctttt
     3361 ctgcgtaata cgcaagccag gcgttgaccg cctcaaaatc attgcttgcg cccaacgtgt
     3421 tggccatgtg gctgcgaaac tcaccactgc tgcccgagag taccgctgaa ccctggatgc
     3481 gctccagact catctgcacc aggctgccct gaccaaactg ccccaaacct gagatcaccc
     3541 gcgcagctac cagttgaccc tgcgcgtttt ggcgtaaaag tcgcctcgat ttgggttcaa
     3601 gagtgctctg agcaataccc atgttcagat gctcggcttg catctgcagc caggccacca
     3661 cttggtccgc gcgttgtacg ccaaagcctt tgatccgggc atgccagcgg tagccataga
     3721 cattgacaaa gcgcaccagg tcgcccaagc tcagcagtcc cacgttgcgc aggcatttgg
     3781 tcaccggcct ggcaaaccag gattcgagcc ggtcagttgc agcgggtgcc accgacagca
     3841 gcatggccaa gtggttgagc gcatgcaccc gctcaccggc cgggtccttc aagctgctgg
     3901 ctgattcaag gaacgcgccc atatccaggc caaagtgcgc tttgtactca atgagcaagt
     3961 cggcttcact ccaggcatct ggatcaaacc cttgctcctg cacccagtct tgcaggctgg
     4021 gcaacacgac cgctggtttg agcgttgcat cactgcgcag gaggtcgatc aagggggtga
     4081 ttttggagga ctctggcagg gtggcatcga ggtggcgagc ggcatcgatg acgtgcttga
     4141 gcaagccctc acgacggttg tggatgtagc gcagatcaga gctggtctca ctccaggcca
     4201 ggtagcgatc aaaggcctcg cgcaggtcca gtcccagcag actgcagcgc aggaaggcaa
     4261 agtggtgaat acccaggctg cgcgcatcct gcaccacctg cgctcgcgag cccaggggcc
     4321 gcccgctctt gcgcgctggc cgcgaatgaa acaggggctc aaacgaatcc cggccatccg
     4381 gggcaattgc cgtcatgcga aggtttcctg gaaatagggg tggttcattt tcaaaaagac
     4441 attcctaaaa ccgcttgggg tgaagcttga tttggttgag ttttcccaaa aaactgccct
     4501 agttgcgcag ggtcataatt ttatgaatgg gcgtggttcg ttcattcaca caaatgtgcc
     4561 tggacgtgcc tcgttaagtg gttgattttg ttgaacgctc aaaaatctgg tcacaaaatg
     4621 gtgtgacgtt ttttcacaca aagtcgtacc tcagttggag tttcaactaa gaccgtactt
     4681 gaattaaacg gaattcccac atcgaccgat taccaatcgg tgcgcatttg ctatggtcaa
     4741 agacgcactc attggcattt gctcagagct cctgccaagc aaatcatcca gggcatgttc
     4801 agacctcttg ctcgggcgaa attcgccggc tacgcagggt caaaatcgtg tgacagatag
     4861 gtacacaatt gtgtgaatgg acgtgggtcg ttcattttca taaaaacgtt cccagcatcg
     4921 ttcgggctga accttgattt agttgaattt ttccaaaata gggccaagaa ttgtgcaaag
     4981 ttaatttgca tcttaacgtt cccaaagtgg aatttttcat gaaaacgtac acgacctgaa
     5041 cgaattctct gtcacatcca ccggatgagt tcccacaacc gaaacttgtc atgttcaagg
     5101 atgtgttgat cggcagaaac ctcaagggtg tcaccgaccg ggaagacttc tcgctcgaaa
     5161 actggccaag accagacgct ggtgcgatgt ccccaactga cctagctctg cttcttaagc
     5221 gaaaagaagc tgttgagcgc tatttgcagg caacctctag cgccacgatc tacacggcca
     5281 cccgtcttgc ccctcgtagg caattcatcc tgcttggccc acccttgttc aggcagggac
     5341 acttttatga aaaacctggg aacgttttta tgcaaatgga cgtgggtgat ttcctaacaa
     5401 ccgaattaat acaattaatc ttgcgtcatg attataagga taatcatgac gcatttggaa
     5461 cggattgatt cagagagggc agggcagggc ggtttttcgg cggctaatca ggcaatggca
     5521 gggcgtcggc acaagcagag ttgaccctgc tttgcaacgg gggcagggta gagcgtctga
     5581 atgctcccaa gcccagactc ccagaaagtt gcggcacccc atctgcgcac ttctcaggtt
     5641 ttggcggggt tgggctaaca acccctttaa ggggataatc tagccaatgg aaaaaaatac
     5701 accgagctat tcactggctg acatactggc tcagatgcag tcggtggcgg atatgaattt
     5761 gaccatttcg gccaggacag gtattcgtga ttctgggatg ggccagatgg atgccttggc
     5821 agttgtgcaa agtttgaccc gcaagcactt ctttaagagc atgaccactc atgcggatta
     5881 tcgagtctgg caagacgtgt atcacgggca atggggcgat ctgtcgctct acatcaagtt
     5941 ccaacgggcg agcgagtatt ttgttgtttc attcaaggag ttgtgacatg gcaagcaaat
     6001 ggaatgatca gccctgccac ctgtgtggcg cgggcacact gcacgatggc gtgaagttag
     6061 ttgagcaggc gtacaagggc aagattttca aatctgagtc acgcggggca ttttgtgacc
     6121 attgcgcaga tggatttgta gagttcgatc aagacgaaga atcggcctgg ttagcttttc
     6181 gcaatcaggt ggacgctgca gaagtggctg atttggccag gattcgcaag aagctcaaac
     6241 tcagtcaact ggaggcagct cgactggctg gtggtggcaa aaatgctttt tcaaggtatg
     6301 agcgaggcca ggcaaagccg gttgctgctg tgatcaatct ctttcgcttg ctcgatggtc
     6361 accctgaatt gctgagtgag ttgcgtacct gacgacaata agctggcaac tggaactggt
     6421 atttaagggg tccaagccca aagggcggaa tttgtcgcaa gcgttaacgg ggaaacattt
     6481 gttcgtcggt cctcaatcag gtggtgctgt accagcgcct ggtccactgt ggcgactgcc
     6541 attgagaagg acgcacctgc cacgacggaa aagctgcgaa gggccagccc acattggatg
     6601 cgcgccacac acatgccact cacgcgttag cgcgtggtgc attgaccacc gtgcgcgaga
     6661 atttgcacca tgctgcgtcg tttgccacta cttccgtctg cctgcacacc gacaaggtca
     6721 aacgggcacg acagataggg gatgcatttg ctttgcatcc atagatttgc caattaaaag
     6781 tggcaccgga atgtgaggat aacggttaag ttggaaattc gagggttaac ccttctttct
     6841 tcccgttact atttgcatca aaaccgagat catcccattt cgggcacctg atatcctaag
     6901 atttcgcggt ttccggctta gcactccaaa gcacgttaag ggcggtttga agctgcagtc
     6961 gacctgtcgg gacttgagca accaaggttc ctccacggct taaaagctgc cttatttttg
     7021 agcaccatgg ggaaaaccca tggctgctca cgttttggca tcttgtgcac caccttatcc
     7081 acagcataac cacagagatt gggggcaacc agtgtcatta gtcgcttgag ccgaatggca
     7141 aaacgctgca agtgcgcttg gaggcatctg ctttgtgctt ttctgtgacc tcgccattgg
     7201 actcgccatt gatgccgtag cgctgcgcaa atttcaaaac acccgccagg tactctgggc
     7261 gcgaggtatg gccgttgagt acttttgcga gcatctggtg gagtgtggtt cgagagcaat
     7321 cttgaaactc tggctgcgct tgcagggttg caataatggt tttatggatg ccaacggcca
     7381 gcggcttcag gcaggcaaca gagtcgattt cgatgatttc tgccatgatt ggcttgcttg
     7441 cgaaatacgc tccgactatc ggtgtaagtt tttcgatttg ttctctcttg cgcgctgaca
     7501 gctcggcttc gtgttggatg ttttcctgct ttttgagttc tttttctgcg ttcgggattg
     7561 gtttgagctt ttttgaggac tcaggggctt tggctggcac tgcggcgggc tttggcaccg
     7621 gcactggggt gactgcgggt gcaattgccg attttttgag agttagcttg gggcgttcag
     7681 tgatcatttt tgttcctctt atgttcgggt tt
//
LOCUS       DAOSLL010000155         7645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8829_length_7645_cov_4.522661, whole genome shotgun sequence.
ACCESSION   DAOSLL010000155 DAOSLL010000000
VERSION     DAOSLL010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7645
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8829_length_7645_cov_4.522661"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            308..3088
                     /locus_tag="PLB25_08600"
     CDS             308..3088
                     /locus_tag="PLB25_08600"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HOE41671.1"
                     /translation="MTDVSALLQDKINQDLQFLLTCLHEVLLELGEHDVATNIPWRTD
                     AAPAVQPVSVEQACQLHSIAFQLLGMVEENAAVQLRRTMETANSPAYERGLWGNTLMR
                     LKAEGYDESTIAAGLAAVHVEPVLTAHPTEAKRTSVIVKHRAIYLLLVKKENPILTPT
                     ERQQNRDEIKELLELLWRTGNIYLEKPELRSERRNVIHYLKNVFPETLPILDNRLKAA
                     WLNQGFSLDTLQKSQFPKLGFGTWVGGDRDGHGLVTAEVTAESLQDLRLNALAVILRR
                     LVDLAAKLSLSDQLQPVPPGLRQWLGERADRLAVTGAQALARNPIEPWRQALNLMIAS
                     LPLAVVRDHAVTLHDATGGYHTPAELSADLALLDQSLREVGAHRLADMEVFNVRRLVE
                     TIGFHLAVLDVRQNSAFHDRAVGQMLAGAGLQDTDFANWPEENRLTFLVAELASPRPF
                     LLPGLRVGTEADAVLDCYRVLATHIQAYGADGIGSLIVSMTRSCSDLLTVYLLAREAG
                     LLVYESSGCRCLLRVVPLFETIDDLLGAAKILHPFLAHPITASTLRAASPSNTASGGK
                     PQQQVMVGYSDSCKDGGILASQGALYRAQRELTQVGDQHGVDLCFFHGRGGSIGRGAG
                     PTFRFLEGLPHGSLRGTFRMTEQGETISQKYSNLLTATYHLELQLAGVFGMTLRASEQ
                     PQSAPEQSRIMDMLVASSMNKYQQLITGDGFMDFFAQATPIDVMEQLRIGSRPARRTG
                     RRTLADLRAIPWVFSWNQSRYLLPSWYGAGTALHQLQQSEPQLFALLCADLNRFALLR
                     YVFMNIELAVLQADLGVMHAYAELVEQVEVRERFLAQIDAEFDLTARLLQLLFQQPIA
                     QRRPTQLAALQLRQAPLAALHRQQIDLLRQWRQEQHNDGISDSTSDGASKLLGQLFLS
                     VNAIAGGVRNTG"
     gene            3240..3488
                     /locus_tag="PLB25_08605"
     CDS             3240..3488
                     /locus_tag="PLB25_08605"
                     /inference="COORDINATES: protein motif:HMM:NF014522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HOE41672.1"
                     /translation="MLKDKAKLTSVLTCHLVAAKGMAARGKAGKVKAVQGSDLTVTTT
                     GAVKVNNAYVVQADIVADNGFIHVIGSVIIPKQGLSPR"
     gene            complement(3695..4114)
                     /locus_tag="PLB25_08610"
     CDS             complement(3695..4114)
                     /locus_tag="PLB25_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOE41673.1"
                     /translation="MRKYPQNSPQAAARIVALTLVADGDVGQAELALLDELVVHQQLG
                     LTRRALHVVIDRFCEDLLSSKQLAWAEACPVDEYTLASLMGEIDNPALRRKVLDLCAR
                     LAMVDDHVAEGESMVLSAALAHWGLHDQMLTASMYPK"
     gene            complement(4254..4532)
                     /locus_tag="PLB25_08615"
     CDS             complement(4254..4532)
                     /locus_tag="PLB25_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012356093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HOE41674.1"
                     /translation="MKCPTCPDITLVMADRQGIEIDYCPACRGIWLDRGELDKLLERS
                     ASAAVPAMSAPMSAAQGRHRPDFEDSGYRDAQRYQRDRKKSWLNEIFD"
     gene            complement(4560..5273)
                     /locus_tag="PLB25_08620"
     CDS             complement(4560..5273)
                     /locus_tag="PLB25_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019142292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HOE41675.1"
                     /translation="MNLTHSPQSPGNSSQYPLVLPAEASAASHSTDARRVLRNTYALL
                     AMTLLWSAGVAVATLALQLPAPGLMLTLVGYFGLLYAVFKLQNSGWALPAVFAMTGFM
                     GYTLGPLLTHSLALPGGAQTVTLALAATGATFLALSGYVLLTRRDFSFMGGFLFVGMV
                     IALIAGIAAMVFQLSALGLAVSAMVALLSAGLILFETSRIVNGGETNYVMATVSLYVS
                     VFNLFTSLLSLFGLGGPGD"
     gene            complement(5305..5646)
                     /locus_tag="PLB25_08625"
     CDS             complement(5305..5646)
                     /locus_tag="PLB25_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HOE41676.1"
                     /translation="MQITFESRDADGSQLRDVAVERVRFALRRLTAWVPQAKVQFSDV
                     NGPRGGVDKRCQVELRSDSVGTVVIASLARDWRTALDRSLARATRVLTRSLQRIQKPA
                     RGRTARLALDS"
     gene            6117..6596
                     /locus_tag="PLB25_08630"
     CDS             6117..6596
                     /locus_tag="PLB25_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41677.1"
                     /translation="MSVIKFSERFFAMGFAAIVILFAGCAVTLIAFSGIDLWRAVQPN
                     EALKLTNRFDLILDCIAMLTIAMAAMELAQTIVEEEFDHTTRLTAAARAHRVLSRFLV
                     VVVVSLAIESLVAIFKLMHEDPARISDAAYIAFAAAALLVAWGIFIKLSQQGQTRAD"
     gene            complement(6685..7101)
                     /locus_tag="PLB25_08635"
     CDS             complement(6685..7101)
                     /locus_tag="PLB25_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOE41678.1"
                     /translation="MRSYPRNSPEAAARIVALVLISDGHISRTEYDALNQPDGVSAFG
                     LAPETMHGIVQTLCEDMLMAGFDGNSILSHIEDGTLTPMLAEVDEPRLQAEVLRVARC
                     AVNADQHHAEGETAMLDAISRIWQTSPVTPVGRADA"
     gene            7355..>7645
                     /locus_tag="PLB25_08640"
     CDS             7355..>7645
                     /locus_tag="PLB25_08640"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF013113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HOE41679.1"
                     /translation="MSTPFNYKHLYYFWVVAKEGGIAILKGTMAPEGAVVKQGSVDAS
                     MMKFSGTARVFDNEGDAMKYLFQGKVQPGDILVVRYEGPKGGPGMRETLALTA"
BASE COUNT         1548 a         2265 c         2323 g         1509 t
ORIGIN      
        1 ggatggccgg ctgcacgact tgcaaaccgc gctgaaatcc ggccctgaac tcgacctggt
       61 gattgacaaa gccgtgcagg cggtgcagtc gcgctacctg aacgccccac aagaatgact
      121 ttgcaagcta cgtaaaatat agctgacagc gcatataggt aaaggtctgg aggctgattt
      181 gacatgtaag catgaagcag ccgacagcat gcctgccgtc gccataaatc gactctttgg
      241 ccagtttgag cgatgacatt ccccactttc tttcacgccc ccccaaccca aaagaggaga
      301 aaacgccatg accgatgtca gcgctttgct acaggacaaa atcaaccaag acctgcaatt
      361 tctgctgacc tgtttgcacg aagttctgct tgaactcggc gagcacgatg tggccaccaa
      421 cattccgtgg cgcactgatg ctgcgcctgc ggtgcaaccg gtgtcggttg aacaagcctg
      481 tcagcttcac tcgattgcct ttcagttgct gggcatggtt gaagaaaacg cggcggttca
      541 gcttcgccgc acgatggaga cggccaacag cccggcctac gagcgcgggc tttggggcaa
      601 tacgctgatg cgcctcaaag ccgagggtta cgacgaaagc accatcgccg ccggtctggc
      661 tgcggtgcat gtcgagccgg tgttgacggc ccacccgacc gaggccaagc gcacgtcagt
      721 gatcgtcaag caccgcgcca tttacctgct gctggtcaaa aaagaaaacc cgatcctgac
      781 cccgacggag cgacagcaaa accgcgatga gatcaaggaa ttgctcgaac tgctctggcg
      841 caccggcaac atctatctgg aaaagccgga actgcgctcc gagcgcagga acgtcatcca
      901 ttacctgaaa aatgtgttcc ctgaaactct gcccattctg gacaaccgct tgaaagctgc
      961 ctggctgaac cagggctttt cgcttgacac cttgcagaaa agccagtttc caaaactcgg
     1021 ctttggcacc tgggtgggtg gcgaccggga cggccatggc cttgtcactg ctgaggtcac
     1081 cgccgaatct ttgcaggatt tgcgcctcaa tgccctggcc gtcatcctgc gtcggctggt
     1141 cgatctggca gccaagctca gtctgtctga tcaactgcag ccggtgccgc ctggcctgcg
     1201 gcagtggctc ggcgaacggg cggaccgcct ggctgtcacc ggtgcacagg cgctggcgcg
     1261 caacccgatt gagccctggc ggcaagcgct caatttgatg attgccagcc tgccgttggc
     1321 ggtggtgcgc gatcacgcgg tgacgctgca cgacgcgacc ggcggctatc acacaccggc
     1381 ggagctgtcg gctgatctgg ccctgctcga ccagtcgctg cgcgaggtcg gcgcacaccg
     1441 tctggccgac atggaggtgt tcaacgtcag gcgactggtc gaaaccatcg gctttcatct
     1501 ggcggtgctt gatgtccggc aaaacagtgc tttccatgac cgcgcggtgg ggcaaatgct
     1561 ggccggtgcc ggtctgcaag acactgactt tgccaattgg ccagaagaaa accgcctgac
     1621 ctttctggtg gctgaactgg cttcgccccg gcccttttta ctgcctggac tgcgcgttgg
     1681 caccgaggcc gatgcggtac tggactgcta ccgcgtgctg gccacgcata tccaggcgta
     1741 tggtgccgat ggcattggct cgctgattgt cagcatgacc cgaagctgct ctgacttgct
     1801 gacggtttat ttgctggcgc gcgaagccgg gctgctggtg tatgagagca gcggctgtcg
     1861 ctgcctgctg cgggtggtgc cgctgtttga aaccattgac gatctgcttg gggcggccaa
     1921 aattttgcat ccgtttttgg cgcatccgat caccgccagc acactgcgcg ctgcaagtcc
     1981 aagtaacaca gccagtggcg gcaaaccaca gcagcaggtg atggtcggct acagcgacag
     2041 ttgcaaggac ggcggcattc tggcaagcca gggcgcgctg taccgggcgc aacgcgaact
     2101 cacgcaagtc ggcgatcagc acggtgttga tttgtgcttc tttcacgggc gtggtggctc
     2161 catcgggcgc ggtgccggcc cgactttccg gtttctggaa ggcttgccgc atggttcgct
     2221 gcgcggaaca tttcgcatga ccgaacaggg cgagaccatc tcgcagaagt attcaaacct
     2281 gctgaccgcc acctaccacc tggagttgca actggccgga gtgtttggca tgacgctgcg
     2341 cgcatcagaa caaccccaat cggcaccgga gcagtccagg atcatggata tgctggttgc
     2401 ctccagcatg aacaagtacc agcaactgat cacgggcgat ggcttcatgg acttttttgc
     2461 ccaggccacg ccgattgatg tgatggagca actccggatt ggctcacgcc cggcgcggcg
     2521 caccgggcgc cgcacgctgg cggacttgcg tgccattccc tgggtgttta gctggaacca
     2581 gtcacgctac ctgctgccaa gctggtatgg cgctggcacc gcgttgcacc agttgcagca
     2641 atcggagcca cagctttttg ccctgctgtg tgccgatttg aatcggtttg cgctgctgcg
     2701 ctatgtcttc atgaacattg aactggcggt gttgcaggcc gaccttggcg tcatgcatgc
     2761 gtatgcagaa ctggttgaac aggttgaagt tcgggagcgt tttctggctc agattgatgc
     2821 ggaatttgac ttgaccgcca ggctcttgca acttctgttc cagcagccca tcgcgcagcg
     2881 gcgaccgaca cagcttgccg ccttgcaact gcgccaggca ccgctggcgg ccttgcatcg
     2941 gcaacagatt gacttgctgc ggcaatggcg gcaggagcag cacaacgatg gcatcagcga
     3001 tagcaccagc gatggtgcca gcaagctgct gggccaactg tttttgtcgg tcaacgcgat
     3061 tgccgggggt gttcgcaaca ccggctaact tgacctgtcg gcatccgcaa atcgccaacg
     3121 cgggagcgcg ggcaactcta aaaccctcac cacggcattg acggctgcgg gcttgttggc
     3181 gttttcgccc ccacagaaac gaagcatttg ccaaagttcc caaagctgat ctggacgcgc
     3241 tgctcaaaga caaagccaaa ctcaccagcg tgctgacctg ccatctggtc gcagccaaag
     3301 gcatggctgc ccgtggcaag gcaggcaaag tcaaggcagt tcaagggtct gacctgacag
     3361 taaccaccac aggcgctgtg aaggtcaaca atgcctatgt ggtgcaagct gacatcgtcg
     3421 ccgacaacgg tttcatccac gtcattggca gcgtcatcat cccgaagcaa ggcctatcgc
     3481 caaggtgagc gcaaagctgc aagtgcagtc ttctgccgac aaggcaggcg ccatgacaca
     3541 tctcgtgatg ccgcagcccg gcatacaaat gcaccgtgat gcacgcggcg caatgagatt
     3601 tggctgcatt tgaaccaggc cctctgaacc gcccaaagaa tgaacggcag cggtgacgcg
     3661 ctggtctgcc tgaaccgcca gcgcgtgcgt gaccctattt tgggtacatc gaagcggtca
     3721 gcatctggtc gtgcagaccc caatgcgcca gcgcagcact cagaaccatg gactcgccct
     3781 ctgccacatg gtcgtcaacc atggcaagcc tggcgcacag gtccagcacc ttgcggcgca
     3841 aggcggggtt gtcgatctcg cccatcaagc tcgccagggt gtattcgtcc accggacagg
     3901 cctctgccca ggccagttgc ttgctcgaaa gcaggtcctc gcaaaatctg tcgatgacga
     3961 cgtgcaaggc ccggcgtgtc aggccaagct gctggtgcac caccagttca tcaagcaggg
     4021 ccagttcagc ctggcccaca tcgccatcgg caacaagggt cagggccaca atgcgggcag
     4081 cggcttgcgg gctgttttgg ggatatttgc gcatatttgt tttttggcct tgaggccgtt
     4141 ggggatggat ggccgagtcg gccgcaccgg ctgtccacgg gttgacggca tgcgatcagt
     4201 ggcgagattg aggggcgaca ccgaagcaac tttgcactgc ggccaattct ggttcaatca
     4261 aaaatctcgt tcaaccacga cttcttgcgg tcgcgctggt aacgctgggc gtcacggtag
     4321 cccgagtcct caaagtcggg gcggtggcgg ccttgcgctg ctgacatggg cgctgacatg
     4381 gcgggtactg cggcactggc cgaacgctcc agcagcttgt cgagttcacc ccggtccagc
     4441 cagatacccc ggcacgcggg gcagtagtca atttcgatcc cctggcgatc agccataacc
     4501 agggtgatgt cggggcaggt cgggcatttc atggtcttct cctttgcgcc agtcgtggct
     4561 tagtcgccgg gtccgcccag gccaaacagg ctcagcagac tggtaaacag gttgaatacc
     4621 gagacataca ggctgaccgt ggccatcacg taattggtct cgccgccgtt gacgatgcgg
     4681 ctggtctcaa acagaatcag gccggccgac agcaaagcca ccatggccga taccgccaga
     4741 cccagtgccg acagttgaaa caccatggcg gcaatgcccg caatcagcgc aatcaccatg
     4801 ccgacaaaca gaaagccgcc catgaagctg aagtcgcgcc gggtcaacag cacatagccc
     4861 gacaacgcca gaaaggtggc accggtggca gccagcgcca gggtaacggt ttgtgcaccg
     4921 ccgggcagcg ccagcgaatg cgtcaacagt ggccccagcg tgtagcccat gaagccggtc
     4981 atggcaaaca cggcaggcag cgcccagccg ctgttttgca gcttgaagac ggcgtacagc
     5041 aggccaaaat aacccaccag cgtcagcatc agtccgggcg cgggcagttg cagcgcgagc
     5101 gtggccaccg ccactccggc gctccatagc agggtcatgg ccagcagcgc gtaggtgttg
     5161 cgcaggaccc gccgagcatc ggttgaatgc gaggctgcgg acgcttcggc tggcagcacc
     5221 agcgggtatt ggctcgagtt gccaggcgac tgcggtgaat gggtgaggtt catgatggat
     5281 tttccttttc tgaagtgtcc agggttaact gtcaagggcc agtcttgcgg tgcgaccacg
     5341 cgccggtttt tgaatgcgct gcaagctgcg cgtcaaaact cgtgtggcac gggccagcga
     5401 gcggtccagc gcggtgcgcc agtcgcgtgc cagcgaggcg atgaccaccg tgccgacgct
     5461 gtcggacctg agttcgacct ggcagcgctt gtcaacacca ccgcgcgggc cgttgacatc
     5521 cgagaactgc accttggcct gcggcaccca ggccgtcagg cggcgcagtg caaagcgcac
     5581 ccgctccacc gccacatcgc gcagttgact gccatcggca tcgcgggatt caaaggtgat
     5641 ctgcattgag ttttctccag tgggttaagg aagacccaac gataaggggc aaagcgcttc
     5701 ttgaaaagca gataaattct tatcaaaatt ccttaaaaac ctgaccatcg gtcagatctc
     5761 ggcgatggca aacccgagcg tgagcgagcg actgatatgg ccgatggact gcagtggccg
     5821 ggtccaggcc aaggcggcgc caggtcgatg gaggatggtg aagaaaaatc tgcctgcagg
     5881 atcggaaacc gtccgtcctg agcctggcgg tccagcccga ccacgcggta gctgccgctg
     5941 agcgcgcgtg caagagactg cccgaggttg ccgctggcac cggtgatcag gaccagcggg
     6001 cgttgctgtg aggtgttcgt gggttgggct tcctgtggtg taaatgtgtt agcgttcgca
     6061 gtctgaaagc gggcgccggg tgcccgttgg tcgcatgcaa cttcaaccaa tcagccatgt
     6121 cagtcatcaa gttctccgag cggtttttcg cgatgggttt tgccgccatc gtcatcttgt
     6181 ttgccggctg cgccgtcacc ttgattgcct tctcgggcat cgatctctgg cgtgccgtgc
     6241 agcccaacga ggcgctgaaa ttgaccaacc gctttgactt gatccttgac tgcattgcca
     6301 tgctgaccat tgcgatggcg gccatggaac tggcccagac catcgtcgaa gaggagttcg
     6361 accacacaac ccgactaacc gctgcagcgc gcgcgcaccg tgtgctctcg cgctttctgg
     6421 tggtggtggt ggtttcgctg gcaatcgagt cactggtggc gatcttcaag ctgatgcatg
     6481 aagaccctgc caggatttcg gacgctgcat acatcgcttt cgcagcggcc gccttgctgg
     6541 tcgcctgggg catcttcatc aaattgagcc agcagggcca gacgcgggcc gactgagctg
     6601 ctctcccacg gcctgcgcca ggcttggttt cgggtgctgg cgcaggccgg atcatggcgc
     6661 gtcctcaatg cggctttagc tcacttaagc atcggcccga ccaaccggcg tgaccggact
     6721 ggtttgccag atgcgactga tcgcgtcgag cattgcggtc tcgccttcgg cgtggtgctg
     6781 atcggcgttg acggcgcacc tggcaacgcg caacacctcg gcttgcagtc gcggctcgtc
     6841 cacctcggcc agcatcgggg tcagcgtacc gtcttcaata tgggacagta tcgagttgcc
     6901 atcaaagccg gccatcagca tgtcttcgca cagcgtctgc acgatgccat gcatggtctc
     6961 aggtgccagg ccgaaagccg acacgccatc aggctggttc agtgcatcgt attcagtgcg
     7021 gctgatgtgg ccatcagaga tcaggacaag cgccacaatg cgggcggcgg cttctgggct
     7081 attgcggggg taggatcgca tggtggtttt cctgggtaga taagtagatg ggtaaaacaa
     7141 tccgaatgga ttggctcatg cggccaggct gggcagcgcc aggcgcacac aagccttgga
     7201 cacatcgtgc atttggctgc tttgggcagc gccgaattca atcgtgcttg gcattgagca
     7261 ttctccagtg ggttaagaga gacttgactg tagaggtcaa acagcctctt taaaagcaga
     7321 taatttgtta tcaaaattcc taaaaaaact gatcatgagc acgcctttca actacaagca
     7381 tctctactac ttctgggtgg tggccaaaga aggcggcatc gccattctta agggaacgat
     7441 ggcccccgag ggcgcggtcg tcaagcaggg gtctgtggac gcgtcgatga tgaagttctc
     7501 cggcaccgcg cgcgtgttcg acaacgaagg cgacgcgatg aagtaccttt tccagggcaa
     7561 ggttcagccc ggcgacatcc tggtcgtccg ctacgaaggc cccaaaggcg gccccggcat
     7621 gcgcgagacg ctggcgttga cggcc
//
LOCUS       DAOSLL010000156         7603 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8888_length_7603_cov_4.947668, whole genome shotgun sequence.
ACCESSION   DAOSLL010000156 DAOSLL010000000
VERSION     DAOSLL010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8888_length_7603_cov_4.947668"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1619)
                     /locus_tag="PLB25_08645"
     CDS             complement(<1..1619)
                     /locus_tag="PLB25_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41680.1"
                     /translation="MSPTDMDADLQTHIAFYLTGKRPDTGLDAPDGLDLRPALLAGYR
                     DLTQLRYDFPLVLVSGQPDGAYVKPMSGLFDGILARVAQGTDAERIHKHVLHLEQAIR
                     TQVAAGASGLFSVLWDEAAAALGQGQPLLTESLSRARANRQVDGELVDCQVNLPGDLI
                     GHAWGLTQAQRAAQFGKDIRRLVLKLSDILKADFANSDAGKSAENLKSSFGSGPKDSF
                     DFEAMSRILTQPARKKNLSQSRRDRVTGLLATLQSQQFFATPESAAGSVYCFAFDTCS
                     AALQAYRERLPKLIELARAVAVAELEVKGEYSEARHDPVFDGFGDNGLQASDLALFPD
                     YLVRLNVRDLSGPEQSTLIEILSVDLPVKILVQTDDVIEESPLGNGHLAFALRSRQLA
                     GMAMGLSGVFVLQSPASSLYQLRGQIQRGLDFSGPALFCVFSGANATTGDLPRYLVGA
                     AALESRVFPAFCFDPSAGRDWAARFSLAGNPQAELDWPVHAFAYEDEQHQAVSTQMPF
                     TLIDFVACDARYARHFARVPRAHWSATLAPAG"
     gene            complement(1874..2827)
                     /locus_tag="PLB25_08650"
     CDS             complement(1874..2827)
                     /locus_tag="PLB25_08650"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOE41681.1"
                     /translation="MTCSTTDCLLELYDEQHELEDYQSGVPRWCTGCGDNAILAAVQR
                     LCRDEGLRPEKTVFVSGIGCSSRFPHYMKTYGFHGIHGRALPVAEGIKMVRPDLTVFV
                     NTGDGDCCSIGAAHWIHAIRYNMNLTVFLHDNQIYGLTKKQASPTSPIGTKSNTTPRG
                     SYLDALNPLSVTLGVANASFVAQAVDWIPESLFEIVKAAYHHQGLSFVRILQRCPEWL
                     PKSLDPWMQDPDKIALLHHENALHLSAGLSKTYKNQWLHDPLDMNRAREIASSEDVIP
                     VGILYQNRDIPRYEETRQTGVLRTLDTVRQGFEAELDKYTV"
     gene            complement(2830..4716)
                     /locus_tag="PLB25_08655"
     CDS             complement(2830..4716)
                     /locus_tag="PLB25_08655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HOE41682.1"
                     /translation="MTSTDTLTSAAAKTITTLKEHIVEIISDSGEGAQRCGQSLGSIA
                     ARMGNGIWTTEIIPAEIRPPARSVAGASGNRIRIGSGYITNGGDQTDLVVAFNEQVLL
                     GRVRDRELKPGCLIFLESSWRKHTDLNVSMAYIQTHDKLVAAGYKVIEFPMEQQCQLL
                     GADPKRGKNMFALGMLCYLYSFELQMARDQIALTFGKKAQSVIDANVKLLEAGHQWGA
                     THLDFNFCIPATPSAQPQIVTNGNTALALGVMASGMELCSMYPITPATSASHYLSEVF
                     EKVGGIVHQAEDEIAACAFAIGASYASKCTITITSGPGYSLKQEAIGLAVMAEIPLVV
                     VNVQRGGPSTGQPTKVEQGDLMCAIFGSHGDAPKVVMAPATIEDCFYSVITARRIAEA
                     FNMVVVVLTDASLSSSQQPFNRPQFNDAWLAPPYDQSPVAEGARAYDWDATTGLARRF
                     IPGQPGGMHTVTGLAHDRDSHVAYDPDINQESLRARSLKLAALQKTLTAPEVFGEANG
                     DLLLVGWGSTQGAIEEAVETLRAEGQKVSSIHLRFLQPMQPGIKAIMQRFKQVMTIEI
                     NWSDSPDEAIIDESNRRYSSLAILLRSRCLIDVDCWSEVRGQPIKPSTIRRIALAKLS
                     PQGK"
     gene            complement(4732..6225)
                     /gene="gltA"
                     /locus_tag="PLB25_08660"
     CDS             complement(4732..6225)
                     /gene="gltA"
                     /locus_tag="PLB25_08660"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HOE41683.1"
                     /translation="MAKKKTIRSIPQERTPMREQNPAVRAKNFEEVACGYHPDDAMRE
                     SERCLMCPDQPCVSGCPVGIDIPGFIKKINEKDLRGAYDILTETSLLPAICGRVCPQE
                     NQCEGVCTVGESLEPVAIGRLERFVGDAAIKAGWANIPYIEANRFKVGIVGSGPAGMA
                     CAADMAKAGCDVTVFEAFHQPGGVLKYGIPDFRLPNTVIDAEIGNLTKLGVKFECNTL
                     VGRLFTLEQMVSEMDFDTVFVGVGAGYPTMLGIPGDSLNGVLSANELLTRCNLMRAKE
                     FPSFDTPQPIGKRVAVVGAGNTAMDAMRVCLRLGAEKVYCIYRRSRKEAPARAEEVHH
                     AEQEGVEYHWQTHPVAILDDGHGNVRGMRCVRMELGEPDASGRRRPIELPDSEFDFEA
                     DLIVYAIGTNANPIMGQTSRIALNKWGYIQTDLGLATSIAGVFAGGDIVTGAATVIEA
                     MGAGRRAAASMKAYLGIRDSARPFDSGGDKLFGIDIRERNFARVRVA"
     gene            complement(6313..7197)
                     /locus_tag="PLB25_08665"
     CDS             complement(6313..7197)
                     /locus_tag="PLB25_08665"
                     /inference="COORDINATES: protein motif:HMM:NF004862.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HOE41684.1"
                     /translation="MSTFEIVRREDFSDVTYLLEVRHPMMAKAAKPGQFVIVMLGEQG
                     ERIPLTIADFDREQGTITLVIQAVGKTTRQMQQECQVGTSLYALVGPMGIPSPISHAK
                     KVLCVGGGLGVAPVFPQARAFKEKGAYVIGVLGFRNKDLVFWDDRFSDYCDELIICTD
                     DGSVGIQGRVTDGILQAIAKHPDIDEVVAIGPPIMMQSCAETTRPFKIKTMVSLNPVM
                     VDGTGMCGGCRVKIGGQVKFACVDGPDFDGHLVDFDDLMTRLRRYTKVERKALDHWEK
                     SCNLHKSIISNMAAASPA"
BASE COUNT         1519 a         2331 c         2257 g         1496 t
ORIGIN      
        1 ccggcgggtg ccagggtggc gctccagtgc gctcttggaa cccgggcaaa gtgccgggcg
       61 tagcgcgcat cacaggcgac aaagtcaatc agggtaaagg gcatctgcgt ggataccgcc
      121 tggtgttgct cgtcttcata ggcaaacgca tgcaccggcc agtccagctc agcctgcgga
      181 ttgccggcca gcgagaaacg tgcagcccag tcacgaccgg ccgaggggtc aaagcagaag
      241 gccgggaaca cccgcgactc cagcgccgct gcgccaacca ggtaccgtgg caggtcgcca
      301 gtggtggcgt tggcacccga gaagacacaa aacagcgctg gcccggaaaa atcgagcccg
      361 cgctggatct gcccgcgaag ctggtacagg ctggatgccg gtgactgcag cacaaacacg
      421 ccgctaagcc ccatcgccat gccggccagt tgccggctgc gcagggcaaa cgccaggtgg
      481 ccgttgccaa gcggcgactc ctcgatcaca tcgtcggtct gcaccagaat cttgaccggc
      541 aaatcaaccg acagaatctc gatcaaagtg ctttgttccg ggccgctcag gtcgcgcaca
      601 ttgagccgca ccaggtaatc agggaacaac gccagatcgc tggcttgcaa gccattgtca
      661 ccaaagccgt caaacaccgg gtcgtgccgg gcttcgctgt attcaccctt gacttccagc
      721 tcggccacgg ccaccgcgcg tgccaattcg atcagtttgg gcaggcgttc gcggtaggcc
      781 tgcagcgccg cgctgcaggt atcaaaagcg aagcaataga ccgacccggc ggcggattcc
      841 ggggtggcaa aaaactgttg tgactgcagc gtggcaagca gccctgtgac ccggtcgcgc
      901 cgactttgcg acagattttt cttgcgtgca ggctgggtca gaatgcgcga catggcttca
      961 aaatcgaagc tgtccttggg gccggagcca aacgatgatt tgaggttttc ggcgctttta
     1021 ccggcatccg aattggcaaa atcggccttc agaatgtcag acaacttgag caccagccgc
     1081 cggatgtcct tgccaaattg cgcggcgcgc tgcgcctgcg tcaggcccca ggcatggccg
     1141 atcaggtcac ccggcagatt gacctggcaa tcgaccagtt cgccatcgac ttggcggttg
     1201 gctcgcgctc gcgacaggct ctcagtcagc agcggctgac cctgtcccaa ggctgcggct
     1261 gcctcgtccc acaacacaga aaacaggccg ctggcaccag ccgccacctg ggtgcgaatc
     1321 gcttgctcca ggtgcaacac atgcttgtgg attcgctcag catcggtgcc ttgcgccacc
     1381 cgggccaaaa tgccgtcaaa gaggcctgac attggtttga catatgcgcc atcgggctgg
     1441 ccgctgacca gcaccagtgg aaagtcgtac cgcaactggg tcagatcacg gtagccagcc
     1501 agcagcgccg ggcgcagatc aagcccgtct ggcgcgtcca ggccggtgtc gggccttttg
     1561 ccggtcaggt aaaaagcaat gtgcgtttgc aggtctgcgt ccatatcagt tgggctcact
     1621 cttggttaaa tcattgaatc acgggtttcg caacgcacat tgatgcgggt cgcggctttt
     1681 gactgtgcgg cagcttcgcg actggactga ctcaggatgt ggctggcaat aacgccccgg
     1741 tttgcgccag gcaatcggca tgccatgcga ggtgccgcac acgccgcctg gcgagccaga
     1801 cgggtgcctt gccacaacgc agcgcgcccg atcccgactg ggcaaatggg gcctgtcatg
     1861 tcttgccaca tggtcagacc gtgtacttgt ccagctctgc ttcaaagcct tggcgcaccg
     1921 tatcgagggt gcgcaagacg ccggtttgcc gcgtttcctc atagcgtgga atatcccggt
     1981 tctggtacag gatgccgacc ggaatgacat cctcgctgct ggcaatttcg cgcgcccggt
     2041 tcatgtcgag cgggtcatgc agccactggt ttttataggt tttgctcaaa ccggcactca
     2101 gatgcagtgc gttttcgtgg tgcagcaagg cgatcttgtc ggggtcctgc atccaggggt
     2161 cgagcgactt gggcagccat tccgggcagc gctgcaagat gcgcacaaac gacaggccct
     2221 ggtggtggta ggcggccttg acgatctcga acaggctctc gggaatccag tccaccgcct
     2281 gcgcgacaaa tgaggcattc gccaccccca gcgtgacgct caaggggttg agcgcatcca
     2341 gataactgcc gcgcggcgtg gtgttgctct tggtgccgat gggggacgtg ggcgaggcct
     2401 gtttcttggt caggccataa atctggttgt cgtgcagaaa caccgtcagg ttcatgttgt
     2461 agcgaatcgc atgaatccag tgcgccgcac caatgctgca gcagtcaccg tcgccggtat
     2521 tgacaaacac cgtcaggtcg ggccgcacca tcttgatgcc ttcggccacc ggcagcgccc
     2581 ggccatggat gccgtgaaag ccataggtct tcatgtaatg cggaaagcgg ctggagcagc
     2641 cgatgcccga cacaaacacg gttttctcgg gccgcaagcc ttcgtcgcgg cacaggcgct
     2701 gtaccgccgc cagaatggcg ttgtcgccgc agccggtgca ccagcgcggc acgccgctct
     2761 gataatcttc cagttcatgc tgctcgtcat acagctcaag caggcagtcg gtagtggaac
     2821 aggtcatggt cattttcctt ggggcgagag tttggccagg gcaatccggc ggatggtgct
     2881 gggcttgatc ggctggccgc gaacctcgct ccagcagtcc acatcaatca ggcaacgcga
     2941 gcgcagcaag atcgccagcg acgaataacg ccggttcgat tcgtcaatga tcgcctcgtc
     3001 cgggctgtcg ctccagttga tctcgatcgt catcacctgc ttgaagcgct gcatgatggc
     3061 cttgatgccc ggctgcatcg gctgcaagaa ccgcaggtga atcgaagaca ccttttgacc
     3121 ctcggcgcgc agcgtttcaa ccgcttcctc gatggcgccc tgggtgctgc cccagccgac
     3181 cagcagcagg tcgccattag cttcaccaaa cacctcgggg gccgtcagcg ttttttgcag
     3241 ggcagcgagt ttgaggctgc gcgcgcgcag gctttcctgg ttgatgtcgg ggtcgtaagc
     3301 gacatggctg tcgcggtcat gcgccaggcc ggtcacggtg tgcatgccgc cgggctggcc
     3361 gggaataaaa cgccgcgcca ggccggtggt cgcgtcccag tcgtaggctc tggcaccttc
     3421 agccaccggg ctttggtcgt atggcggtgc cagccaggca tcgttgaact gcggccggtt
     3481 aaacggctgc tgtgaagacg acaggctggc atcggtcagc accaccacca ccatattgaa
     3541 ggcttcggca atccggcgtg cggtgatcac cgaataaaag cagtcctcga tggtggcagg
     3601 tgccatcacc accttgggcg cgtcgccatg gctgccaaaa atggcgcaca tcaaatcgcc
     3661 ctgctccacc ttggtcggct ggccggtgct ggggccgccg cgctgcacat tcaccaccac
     3721 cagcggaatt tcggccatca ccgccaggcc aatcgcctcc tgcttgagcg aataacccgg
     3781 cccggaggtg atggtgatgg tgcatttgct ggcgtaagag gcaccaatgg caaacgcgca
     3841 agcggcaatt tcgtcctcgg cctggtgcac gatgccgccg actttctcga acacttccga
     3901 caaatagtgg gaggccgagg tggccggggt gatcgggtac atcgaacaca actccatgcc
     3961 cgaagccatg accccaagcg ccagcgcggt gttgccgttg gtgacgatct gtggctgcgc
     4021 tgagggtgtg gccggaatgc aaaagttaaa gtccagatgc gtcgcccccc actggtgccc
     4081 cgcttcgagc agcttgacat tggcatcaat cacactttgg gcctttttac caaaggtcag
     4141 cgcaatctgg tcgcgcgcca tctgcagctc aaagctgtag aggtaacaca gcatgcccag
     4201 cgcaaacatg tttttaccgc gcttcgggtc agcgccgagc aactgacatt gctgctccat
     4261 cggaaattcg atcaccttgt aaccagccgc caccagcttg tcgtgggtct ggatgtaggc
     4321 catcgacaca ttcaaatccg tgtgcttgcg ccatgaactt tcgagaaaaa tcaggcagcc
     4381 gggtttgagc tcgcggtcgc gcacccggcc cagcagcacc tgctcgttga acgccaccac
     4441 cagatcggtc tggtcgccgc cattggtaat gtagcccgaa ccgatgcgaa tccggttgcc
     4501 gctggccccg gccacgctgc gcgcgggcgg tcggatttcg gctggaatga tctcggtggt
     4561 ccagatgcca ttgcccatgc gggcagcaat cgagccgagc gactggccgc agcgctgcgc
     4621 gccttcgccg gagtcgctga tgatctcgac aatgtgctct ttcagggtcg taatggtctt
     4681 ggccgcagcg cttgtcaggg tgtcagtcga agtcatattg ggtatttcca ttcaagccac
     4741 gcgcacacgc gcaaaattgc gttcccggat gtcgatgcca aacagcttgt cgccaccact
     4801 gtcaaacggt cgggcgctgt cgcggatgcc aagataggcc ttcatgctgg cagcggcccg
     4861 gcgaccggcc cccatcgcct cgatcaccgt ggcggcaccg gtaacgatat cgcccccggc
     4921 aaacacgcca gcaatcgaag tggccagccc cagatcggtc tggatatagc cccatttgtt
     4981 cagcgcaatc ctcgaggtct gacccatgat cggattcgca ttggtgccga tggcataaac
     5041 aatcaagtcg gcctcaaagt caaattcgct gtcgggcagc tcgatcgggc ggcgccgacc
     5101 cgatgcatcg ggctcaccga gttccatgcg cacacagcgc atgccgcgca cattgccgtg
     5161 gccgtcgtcc agaattgcca ccggatgggt ttgccagtgg tactcgaccc cttcctgctc
     5221 ggcatggtgc acctcttcgg cgcgtgccgg tgcctccttg cgcgagcggc ggtagatgca
     5281 atagaccttt tcggcaccca ggcgcaggca gacgcgcatc gcgtccatcg cggtgttgcc
     5341 agcaccgacc accgcgacac gtttgccaat tggctggggc gtgtcaaaac tcggaaattc
     5401 cttggcccgc atcaggttgc agcgcgtcag caattcgttg gccgacagca cgccgttgag
     5461 ggaatcgccg gggatgccca gcatggtcgg gtaaccagcc ccgacaccga caaacaccgt
     5521 atcaaaatcc atctcggaca ccatctgctc tagcgtgaac aggcgcccga ccagggtgtt
     5581 gcattcaaac ttgaccccga gtttggtcag gttgccaatt tccgcatcaa ttaccgtatt
     5641 gggcagtcga aagtcgggaa tgccgtactt cagcacccca ccgggctgat gaaaggcttc
     5701 aaacaccgtg acatcacaac cggccttggc catgtcagcc gcgcaggcca tgcctgccgg
     5761 gccggagccg acgatgccga ccttgaaccg gttggcttca atgtacggaa tattggccca
     5821 gccagccttg atggcagcat caccgacaaa gcgctccagc cgaccaatcg ccactggctc
     5881 aagcgactca cccaccgtgc aaaccccttc gcactggttt tcctgcgggc aaacgcggcc
     5941 acagatggcc ggcagcaggc tggtttcggt caggatgtcg taggcaccgc gcaggtcttt
     6001 ttcattgatt ttcttgatga agccgggaat gtcaatgcca accgggcagc ccgacacaca
     6061 gggctggtcc gggcacatca ggcagcgctc ggactcgcgc atcgcatcat cggggtgata
     6121 gccgcaggcg acttcctcaa agtttttggc ccgcacggcg gggttttgct cgcgcatcgg
     6181 ggtgcgctcc tggggaatgg agcggatggt ttttttcttc gccatgggtt gctaccgttg
     6241 aatgtggaaa ctgcacgcac acctgcccaa acgcatagcc tgcgtgacaa gaaggcgaac
     6301 gaatgagact gcttacgccg ggctggccgc tgccatgttg ctgataatgg acttgtgcag
     6361 gttgcagctt ttttcccagt gatcaagcgc cttgcgctcg accttggtgt agcggcgcag
     6421 gcgcgtcatc aggtcatcaa aatcgaccag atggccgtca aagtctggcc cgtcaacgca
     6481 ggcaaatttg acctggccgc caatcttgac ccggcagccg ccacacatgc cggtgccatc
     6541 gaccatcacc gggttgaggc tgaccatggt tttgatctta aaaggccggg tggtttcggc
     6601 acagctttgc atcatgattg gcgggccaat tgccaccacc tcgtcaatgt caggatgctt
     6661 ggcaatcgcc tgcaaaatgc cgtcggtgac ccggccctgg atgccaaccg aaccgtcgtc
     6721 ggtgcagatg atgagttcat cgcaatagtc actgaagcgg tcgtcccaga acaccaggtc
     6781 cttgttgcga aaacccagca cgccgatgac gtaagcgccc ttttccttga aggcgcgggc
     6841 ctgtggaaaa accggcgcca cacccagccc gccaccgaca cacagtacct ttttggcgtg
     6901 gctgatcggg ctgggaatgc ccatcggtcc caccagcgca tacagcgatg tgccaacctg
     6961 gcattcctgc tgcatctgcc gggtggtctt gccgaccgcc tggatcacca gcgtgatggt
     7021 gccctgctct cggtcaaaat cggcaatcgt cagtggaatt cgctcgccct gctcgcccag
     7081 catgacgatc acaaattgac cgggcttggc tgctttggcc atcatcgggt ggcgtacttc
     7141 aagcaagtaa gtcacgtcag agaagtcctc tcgacggacg atttcaaagg tgctcatttc
     7201 gctcactttc ataggtgtct agactgtttg gacaaacaaa aatcgtgttc accgcctgtg
     7261 caacggtttg cacagctttt accaacgatg taccaaccct gataagcatt agccgtgcca
     7321 gaaatagcgt gaacgcggca aagtggccaa tgacaagcct tgcggattca aaaagaccaa
     7381 actaaccgat tgcgcctttg gtatcagcat caagctggtc gcaagccagc gccaactttg
     7441 ttctgacgca gttctgcctg gctgccaaag gcgtggtcag gaatgaatcc ccgttttgca
     7501 ccagggagtt gggatgcaag gccaggcgcg agacgcgacg tgcaactggc taaggatgtg
     7561 gtcgggaata actccccgtt ttgcaccaag gagttgggat gca
//
LOCUS       DAOSLL010000157         7539 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8972_length_7539_cov_4.609968, whole genome shotgun sequence.
ACCESSION   DAOSLL010000157 DAOSLL010000000
VERSION     DAOSLL010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7539)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8972_length_7539_cov_4.609968"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="PLB25_08670"
     CDS             <1..726
                     /locus_tag="PLB25_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE41685.1"
                     /translation="EAHGKGQERQDHRDLEAALAEGAFREDLFHRLNVIRLRLPALRE
                     RGEDIPVLTRHFLQQSAAQLGVEPKRISEAALAWLGRFNFPGNVRQLENICHWLTVMA
                     PAQMVEPKDLPPEVAVSDAESATDLVTVSVAAAVAAAAAGIAPGTALSVPLITPLPPV
                     IPVMAMSDLADHGWERGLEQDASALLSTDRTDVWDSLTRRFESRLIIAALAATRGRRI
                     EAAQKLGIGRNTITRKIQELGLD"
     gene            complement(818..1822)
                     /locus_tag="PLB25_08675"
     CDS             complement(818..1822)
                     /locus_tag="PLB25_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE41686.1"
                     /translation="MQVGPILIVDDEPQNLAVLEHILSPHHLLVFARDGDEALLAVQK
                     HRPSLILLDIEMPGMNGYAVCRALKTNPATENIPVIFISGKSAEGDEATGFEVGAVDY
                     IVKPVSPAIVLARVRTHLSLVRASHLEKSYHDAIHMLGEAGHFNDNDTGVHIWRMAAY
                     ASALAQACGLAPELCQQIELAASMHDTGKIGISNAILKKPGKLDAAEWLEMKTHPRIG
                     HDILAKSDAPLFVLAAEIALRHHERWDGTGYPDGMAGAAIPLSARIVALADVFDAMVT
                     KRPYKQPWPIDQVMTTLRDSAGSHFEPRLVQVFESILPRILAIKAVWDSKERLATATR
                     "
     gene            complement(1824..6602)
                     /locus_tag="PLB25_08680"
     CDS             complement(1824..6602)
                     /locus_tag="PLB25_08680"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE41687.1"
                     /translation="MTTSPRKRFIVSATVSYALLGLAWIFLSDQLLSVFADVGSVVWL
                     SSAKGVFFVLATSCLFFIALRAVPHDNAADGTAVSHTLAVMLDPARSGRWLHYGFSAA
                     ITLAMLAVQQSIVDANAGSSLLVLFMLPIVLSAMLGGLGPGLLATALSALSSNYLTLP
                     PLGSLRIDHSQDVFQWAVLIASGVVVSLLADQLRRALAKATLDRSLLNAVVGGTSDAV
                     FVKDRQGRYLLANQATAGFLGKPESDIVGHDDTGLMDSGSAQQIVANDAIVMAGRQSA
                     RLEEKVVNLDGKPMVFLVTKGPVFDSLGQVVGLFGISRDITAQVKDDEQRLLLSETFR
                     QSAQAMLFADAQLLITYANPAFCRLFGYQPDELAGQLVSCLCPLTPEAVAEQAAVTRK
                     VMEQGSWSGKVERRAKDGSLIPVAINSGVVRDGQDHLLGFYGSYADLRPELEHDKLLG
                     KLSLAETHRQASDDARLRAEDRLEYRESTFRSMLENMLGGCQIVDFDWRYTFINHAAE
                     RQNRCASETLLGRSVMECWPNFTETEVFALEQACMRERSVQSGDLAFVFPDGQQGWYR
                     VIVQPVPEGIAIYSEDISAQKQLQAELGQFNQQLEAELRQRSQEYLDIYHQAPCGYHS
                     LAPDGTLLRVNQTELDLLGYTREEYLGHHVFEFMTPESAQTARSEMSKFLNSGRVRDL
                     EIDFITKSGQVRPFLVSGDLIRDDQGQPKESNSMLVDNSERKAREQQIRDLNRLLNEV
                     VAALPFGLVVYDEQRRAVLRNTLYGRLLDYPPELIAKEPFYFADSVHYNHARGDYPGR
                     AFDEVLAGFEKFMDERQSIQFERQQANGTYLEVRGLPIANGWTLLTYTDISAHKQAEQ
                     TLHEARQKAEAATDAKSAFLSSMSHEIRTPLNAMLGLAYLLEQLALPGEANDMAHKIR
                     VAGRSLLSIINDILDFSRVESGKLQIENRPFRLGDVLDNLSTIMSGSAGEKDLELIIA
                     APPGKTSQLRGDAMRLEQVLINLAGNAIKFTERGHVELAVSVAAEEAAHVTLRFSVRD
                     SGIGIAPDKQQEIFELFSQVDSSIGRRFGGTGLGLAISRRLVRSMGGELHVTSVPGSG
                     SEFWFELRFEQEQDAWLASPEMAGLKVLVADDNPIAREALCGTVQGLGWQATSVGSGE
                     AAIEYLQTRAQHPGPAEVLLLDYKMPGLDGLATARRIRQELKNDRDLIIIMVTNYASR
                     EQLNHTDRHLADAVLSKPVTGSTLYNAVARALRVRQGGETQPASRVSARLQGMRILVV
                     DDSDINREVAQRILASEGAQVVLANDGQQALDWLTKNPAGVEIVLMDVQMPVLNGYDA
                     TRQIRRSPELVKLPVVALTAGVFMEQQEMASNAGMNSVITKPFDVDAAIALIIRLTGW
                     QPVPPALAAEPTLPGIARPVDDTQDLPGLAVGAALALWKEPAKYRQFLRKFVQMYGDV
                     MPRLRSLESKEAAALVHKFKGPAGYVGLKEVAALAEQVNGDLKKGHDPQPSLQALQLA
                     FDTALNSINRYAPPEASGEPDQPTGFEPQQLAALLARLLAAFDTDRPSAIRPVLAELL
                     QGLPMTRLQAVHTAVENFDFRGGEAATRALAQAMGIDLGG"
     gene            complement(6942..>7539)
                     /locus_tag="PLB25_08685"
     CDS             complement(6942..>7539)
                     /locus_tag="PLB25_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HOE41688.1"
                     /translation="VALLSRLPATDVVRNIPGFDDDMARVISGTFPLAGDDSAVRVIG
                     AYFPNGQEPGSDKFEYKLAWLKGLRDWLRSELQRHPNLVLMGDYNITFDDADVWDPDG
                     MRDQIHCTDEERYELQALIALGLHDAHRLFPQAPKSYSWWDYRDFAFRRNRGMRIDHI
                     LISQALKSRASACWIDKAPRKNERPSDHAPVVLTLDAS"
BASE COUNT         1437 a         2331 c         2273 g         1498 t
ORIGIN      
        1 gaagctcacg ggaagggcca ggagcggcag gaccaccgtg atctggaagc ggcactggcc
       61 gaaggagctt tccgcgaaga cctgtttcac cgcctcaacg tgattcgcct gcgcttgcca
      121 gcgctgcgcg agcgtggtga agacattccg gtgttgaccc gccacttttt gcagcaaagc
      181 gccgcccagc ttggggtgga acccaagcgc atttccgagg cggcactggc ctggctgggc
      241 cgtttcaact ttccgggcaa tgtgcggcaa ctggaaaaca tctgccattg gctcaccgtg
      301 atggccccgg cgcaaatggt tgagcccaaa gacttgccac ctgaagtggc tgttagcgat
      361 gccgagtcag ccaccgattt ggtgaccgtt tcggtggctg cagcggtggc tgctgcggct
      421 gcaggcattg cgcccgggac cgctttgagc gtcccgctga tcaccccgct gccacctgtc
      481 ataccggtca tggccatgtc cgatctggct gatcatggct gggagcgtgg tctggagcaa
      541 gatgccagtg ccctgctcag caccgaccgg accgatgtct gggattccct gacacgccgc
      601 tttgagtcgc gtctgattat tgccgcactc gccgccaccc gtggtcggcg cattgaggcc
      661 gcgcaaaagc ttggcattgg ccgcaacacc atcacgcgca agattcagga acttggttta
      721 gattaaaaac gccttctagc ccttgataga caagcgcaag cagctactat aaagatagta
      781 aatcaaggca atcaacgctg gcccggcagg gtctgggcta ccgggtagcc gtcgcgaggc
      841 gttcctttga atcccagact gccttgatgg ccagaatgcg cggcaggatc gattcaaaca
      901 cctgcaccaa gcgcggctca aaatggctac cagcactgtc tcgcaaggtg gtcatcacct
      961 gatcgatcgg ccaaggctgc ttgtagggcc gcttggtcac cattgcgtca aatacatcgg
     1021 ccagcgccac aatgcgggcc gacagcggga tggctgcacc agccatccca tccggataac
     1081 cggtgccatc ccagcgctca tggtgtctta gcgcgatctc cgctgccagc acaaacagcg
     1141 gcgcgtcact tttggccagg atgtcgtgcc cgatgcgggg gtgggttttc atctccagcc
     1201 attcggcggc atcgagcttg ccgggcttct tgaggatggc gtttgaaatg ccgatcttgc
     1261 cggtgtcgtg catcgacgcg gccagctcga tctgttggca cagctcaggt gccagaccgc
     1321 atgcctgtgc cagtgcgctg gcgtaagcgg ccatgcgcca gatgtgaaca ccggtgtcgt
     1381 tgtcattgaa atgcccggcc tcgcccagca tgtgaatggc atcgtggtaa ctcttttcca
     1441 ggtggctggc gcgcaccagc gacaaatggg tgcgcacccg cgccagcacg atcgccgggg
     1501 acaccggttt gacgatgtag tcaaccgcgc caacttcaaa accggtcgcc tcgtcccctt
     1561 cagcagactt gccactgatg aagatgaccg ggatgttttc ggtggccggg ttggtcttga
     1621 gtgcccggca gaccgcgtag ccattcatgc caggcatctc gatgtcgagc aggatcagcg
     1681 acggcctgtg cttttgtacg gccagcagcg cctcgtcacc atcgcgcgcg aacaccagca
     1741 gatggtgtgg cgacaggatg tgctccagca cagccaggtt ctgtggctcg tcgtcgacga
     1801 tcaggatcgg gccaacctgc atctcagcct cccagatcga tgcccatggc ctgggccagc
     1861 gcccgggtgg ctgcctcgcc acctcggaaa tcaaaatttt ccaccgccgt gtgcaccgcc
     1921 tgcaggcgag tcatcggcag gccttgtaga agctcggcca gcaccgggcg gatggcactg
     1981 gggcggtcag tgtcaaaggc cgccagcaga cgcgccagca gtgccgccaa ctgctgtggc
     2041 tcaaagccag ttggttggtc tggctcgcca gatgcctctg gcggggcgta gcggttgata
     2101 gagttcagcg ccgtgtcaaa ggccaactgc agcgcctgca gactgggctg cgggtcatga
     2161 ccttttttca ggtcgccatt cacctgctcg gccagtgccg ccacctcttt gaggcccacg
     2221 tagccggctg gtcccttgaa cttgtgcacc agcgccgccg cttctttgga ctccagcgac
     2281 cgcagcctgg gcatcacatc gccgtacatc tggacaaact tgcgcaggaa ctggcggtat
     2341 ttggcaggtt ctttccaaag cgccagcgcc gccccaaccg ccagtccggg caagtcctgc
     2401 gtgtcgtcca cgggtctggc aatgccgggc agggtgggct cagccgctag cgcgggcggc
     2461 accggctgcc acccggtcag cctgatgatc agcgcaatcg cggcatccac atcaaacggc
     2521 ttggtgatga cgctgttcat gccggcgttg ctggccattt cttgctgttc catgaaaacg
     2581 ccggcggtca aggccaccac tggcagcttg accagttccg ggctgcgccg aatctggcgg
     2641 gtcgcatcgt agccgttgag caccggcatc tgcacgtcca tcagcacaat ctcgacccca
     2701 gcggggtttt tagtcagcca gtccagtgcc tgttggccgt cgttcgccag caccacctgg
     2761 gcaccctcgc tggccaaaat gcgctgcgcc acctcgcggt tgatgtcgct gtcgtccacc
     2821 accagaatgc gcatgccctg cagccgcgcg ctgacgcggc tggcgggctg ggtttcaccg
     2881 ccctggcgta cccgcaaggc tcgggccacc gcgttgtaca gggttgaacc ggtaaccggc
     2941 ttgctcagca ccgcatcagc caggtgtctg tcagtgtggt taagctgctc gcggctggcg
     3001 tagttggtga ccatgatgat gatcaggtca cggtcgttct taagctcctg gcgaattcgc
     3061 ctggccgtgg ccagaccgtc cagcccaggc atcttgtagt ccagcagcag cacctcggcc
     3121 gggccggggt gttgtgcccg ggtctgcaga tattcgatgg cggcttcgcc agagccgacc
     3181 gaagtggcct gccagcccag gccctgcacc gtgccacaca gcgcttcgcg ggcaatcggg
     3241 ttgtcgtcag ccaccagcac cttcagcccg gccatctcgg gggaagccag ccaggcatcc
     3301 tgctcctgct caaagcgcag ttcaaaccag aactcgctgc cgctgccggg cacactggtc
     3361 acgtgcagct caccgcccat gctgcgcacc aggcggcggc tgattgccag acccaggccg
     3421 gtgccgccaa agcggcgacc aatcgagctg tccacttgtg aaaatagttc aaatatctct
     3481 tgctgcttgt ccggtgcaat gccaataccg gagtcgcgca cagaaaagcg cagcgtgaca
     3541 tgtgcggcct cttcggccgc cacgctaacg gccagctcga catgaccgcg ctcggtgaac
     3601 ttgatggcgt tgccagccag gttgatcagc acctgctcca ggcgcatggc atcgccccgc
     3661 aactggctgg tcttgccagg cggcgctgca atgatgagtt ccaggtcttt ttcaccggcg
     3721 ctgcccgaca tgatggtgga caggttgtcg agcacgtcgc ccagccgaaa cggccggttc
     3781 tcgatctgca gtttgcccga ctcgaccctg gaaaaatcga ggatgtcgtt gatgatgctc
     3841 aaaagcgaac gcccggccac ccggatcttg tgcgccatgt cgttggcttc accgggcagg
     3901 gccaactgct ccagcaggta ggccaggccc agcatggcgt tgagcggcgt gcggatttcg
     3961 tggctcatgc tggagagaaa agcgctcttg gcatcagtgg ctgcttcggc cttttgtcgt
     4021 gcctcgtgca gtgtttgctc ggcttgcttg tgggcgctga tgtcggtata ggtcagcagg
     4081 gtccagccgt tggcgatcgg caggccacgc acctccagat aggtgccatt ggcttgctgg
     4141 cgctcgaact ggatgctttg gcgctcgtcc atgaacttct caaaccctgc cagtacctcg
     4201 tcaaacgcgc gcccagggta gtcacccctg gcatggttgt agtgcaccga atcggcaaag
     4261 taaaacggct ccttggcgat caactctggc gggtagtcaa gcaacctgcc atacaaggtg
     4321 ttgcgcaaaa cggcgcggcg ctgctcgtcg tacaccacca ggccaaaggg cagggccgcc
     4381 accacttcgt tgagcaggcg gttaaggtcc ctgatttgtt gctcgcgtgc cttgcgctcg
     4441 ctgttgtcca ccagcatgct gttggactct ttgggctggc cctggtcgtc gcggatcagg
     4501 tcgccgctta ccagaaatgg cctgacctgc ccgctcttgg tgatgaagtc aatctccaga
     4561 tcgcgcaccc ggccgctatt gagaaatttg ctcatctcgc tgcgagctgt ttgtgcgctc
     4621 tcaggtgtca tgaactcaaa cacatggtgc cccaggtact cctcgcgggt gtagccgagc
     4681 agatcaagct cggtctgatt gacgcgcaaa agggtgccat cgggcgccag cgagtgatag
     4741 ccgcagggtg cctggtggta gatgtccagg tactcctggc tgcgctgtcg caactcggct
     4801 tccaactgct ggttgaactg acccagttcc gcctgcaact gcttttgagc actgatgtcc
     4861 tcggagtaaa tggcgatgcc ttcaggcacc ggctgcacaa tgacgcggta ccagccttgc
     4921 tggccgtctg gaaacacaaa cgccaggtcg cctgattgca cgctgcgctc gcgcatgcag
     4981 gcttgctcca gcgcaaacac ctcggtttcg gtgaagttcg gccagcattc catcacgctg
     5041 cggcccaaca gggtttccga ggcgcagcgg ttttgccgtt cggcggcgtg gttgatgaag
     5101 gtgtagcgcc agtcaaaatc cacaatctgg cagccgccca acatgttctc aagcatgctg
     5161 cgaaaggttg actcacggta ttcaagccgg tcttcggcac gcagccgggc atcgtcgctg
     5221 gcctggcgat gcgtctcggc cagtgacagt ttgccaagca gcttgtcgtg ctcgagttca
     5281 ggccgcaggt cagcatagct gccgtaaaag ccgagcaaat ggtcttggcc gtcgcggacc
     5341 acccccgaat tgatggctac cgggatcagg ctgccatcct tggcgcgccg ctcaaccttg
     5401 cctgaccaac tgccttgttc catcaccttg cgcgtgacgg cggcctgctc ggctaccgcc
     5461 tcaggtgtca acgggcacag acaactgacc aactggcctg ccaactcgtc tggctgatag
     5521 ccaaacaagc ggcaaaaggc cgggttggcg tatgtgatca gcagttgggc atctgcaaac
     5581 aacatggctt gcgctgactg tctgaatgtt tcactcagca acaggcgctg ttcgtcatcc
     5641 ttgacctggg cggtaatgtc ccgcgatatg ccaaacaggc caaccacctg gcccagactg
     5701 tcaaacaccg gccccttggt caccagaaac accatcggtt tgccgtcgag gttgaccacc
     5761 ttttcttcca gccgggcgct ttgtcggccc gccatgacga tggcgtcgtt ggcgacgatc
     5821 tgctgcgccg agccactgtc catcaaaccg gtgtcatcgt gaccgacgat gtcgctttcg
     5881 ggtttgccaa gaaaaccggc tgtggcctgg tttgccagca ggtagcggcc ctgccggtct
     5941 ttcacaaaaa cggcgtccga ggttccgccg accaccgcat tgagcagact gcggtccagg
     6001 gtggccttgg ccagcgcgcg tctgagttga tcggccagca ggctgaccac tacgccactg
     6061 gcaatcagca ctgcccactg gaacacatcc tggctgtgat cgatgcgcaa gctgcccagc
     6121 ggcggcaaag tcaggtagtt tgatgacagc gccgacaacg cggtggccag cagtcctggc
     6181 cccaggccac cgagcatggc actgagcaca atcggtagca tgaaaagcac cagcaaggag
     6241 ctgccggcgt tggcatcgac aatgctttgt tgcaccgcca gcatcgccag ggtaattgct
     6301 gcagaaaaac cgtagtgcaa ccagcgccct gacctggccg ggtcgagcat gactgccagg
     6361 gtgtgagaga ccgcggtgcc gtcggcggcg ttgtcatgtg gcacggcgcg caaggcaata
     6421 aaaaacaggc acgaggtggc aagcacaaaa aacacccctt tggcgctgga cagccagacc
     6481 accgagccaa catcggcaaa taccgacagc aactgatccg acaaaaaaat ccaggccagg
     6541 cccagcagtg cgtagctgac ggtggccgac acgatgaagc gcttgcgagg agacgtcgtc
     6601 ataaaacctc acggaatgtc gccgggccaa gacaaaaacg gcctgtcaag ctacccgcac
     6661 gggtcatcaa gaaccgtcac ccgatggtac tccgtgcccg atgatggatg accattaatc
     6721 ctataacggc atcgttcgtg gtgagcttgc tgaaccaccg gccttgtcac aagcacatcg
     6781 actggttttg gggcgaaaga ttccttcatg cgcggtgggc agtagtgcat caccgggcga
     6841 tgcccggcgg ggactgcggg ggaaggcttc acggttatgt gagttttatg ccgccagccc
     6901 ttgtggagat tgcgctgata gctactataa aaatagctgc atcaagatgc atcgagcgtc
     6961 agcacgacag gtgcgtggtc gctcgggcgc tcgtttttgc gtggtgcctt gtcaatccag
     7021 caggcgctgg cgcggctttt gagtgcctgg ctgatcagga tatggtcaat gcgcatgccc
     7081 cggttgcgcc tgaaggcaaa gtcccggtag tcccaccaag agtagctttt gggtgcctgt
     7141 gggaacaatc ggtgggcatc gtgcaagccc agcgcgatca gcgcctgcag ttcgtaacgc
     7201 tcttcgtcgg tgcagtgaat ctggtcgcgc atgccatcgg ggtcccatac gtcggcatcg
     7261 tcaaaagtga tgttgtagtc acccatcagc accagattcg ggtggcgttg caactcgctg
     7321 cgaagccagt cgcgcaggcc tttgagccag gccaacttgt actcgaactt gtcgctgcca
     7381 ggctcttgac cattgggaaa ataggcgccg atgacacgca ctgccgagtc atcgccggca
     7441 agcggaaaag ttccgctgat gacgcgcgcc atgtcgtcgt caaagcccgg aatgttgcgc
     7501 acaacatccg tggctggcaa tctcgacagc agcgccacg
//
LOCUS       DAOSLL010000158         7536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_8978_length_7536_cov_4.941853, whole genome shotgun sequence.
ACCESSION   DAOSLL010000158 DAOSLL010000000
VERSION     DAOSLL010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8978_length_7536_cov_4.941853"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            581..3169
                     /gene="clpB"
                     /locus_tag="PLB25_08690"
     CDS             581..3169
                     /gene="clpB"
                     /locus_tag="PLB25_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HOE41689.1"
                     /translation="MRIEKLTTKFQEALADAQSLALEKDHAYIEPAHLLVAMLKQDDG
                     PKALLQRAGVNVAGLLNAAEAAVKKLPQVTGQDQVQGGPELGKLLQATEKEALKRGDQ
                     FVAGELLLLALADAKGSLGDAARANGLTRKSLESAIEAVRGGQSVDSANAEDQRESLK
                     KYCVDLTERARMGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEGLA
                     QRIVAGEVPESLKGKRVLSLDMASLLAGAKFRGEFEERLKNVLKDLAKDEGQTIVFID
                     ELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEYRKYIEKDAALERRFQKI
                     LVGEPSVEATIAILRGLKERYEKHHNVEITDPAIVAAAELSHRYITDRFLPDKAIDLI
                     DEAASKIKIEMDSKPEVMDKLDRRLIQLQIEREAVKKEKDEASLKRLELITDEIKSLQ
                     KEIANLDEVWRAEKASAQGSGDLREQIDLLKFKIEELTRKGDFNKVAELQYGKLPELE
                     KRLKEAQAKESTRGEGAAPRLLRTQVGAEEIAEVVSRSTGIPVSKMMQGERDKLLQME
                     VKLHQRVVGQDEAIAAVANAIRRSRSGLSDPNRPTGSFLFLGPTGVGKTELCKALAGF
                     MFDSEDHLIRIDMSEFMEKHSVARLIGAPPGYVGYEEGGHLTEAVRRKPYSVLLLDEV
                     EKAHPDVFNVLLQVLDDGRLTDGHGRTVDFKNTVIVMTSNIGSQMIQNMLGQDYEDVK
                     DAVTAELKNHFRPEFLNRIDETVVFHSLDASHIADIAKIQLAVLQARLAHMDLGLSVS
                     NAAVAELAKVGFDPVFGARPLKRAIQQRLENPLSKLLLEGKFAPKSTIHVEVDTIRAP
                     GQFNFV"
     gene            3403..3948
                     /locus_tag="PLB25_08695"
     CDS             3403..3948
                     /locus_tag="PLB25_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VUT family protein"
                     /protein_id="HOE41690.1"
                     /translation="MQKALSQPLLSLWRPILAMLVVIVWSNYLVQFPINDWLTWGAFT
                     FPVAFLVTDLTNRAVGAQAARRVAWTGFAMAVLVSLALTPWRIAVASGVAFIAGQLLD
                     IVAFNRLRASSWWKAPLIGSVVASVVDTGIFFFLAFAGSDLDWRMLATGDLAVKWLMA
                     ALLLAPYRVMLPRLQLWVPVH"
     gene            complement(4006..5043)
                     /locus_tag="PLB25_08700"
     CDS             complement(4006..5043)
                     /locus_tag="PLB25_08700"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HOE41691.1"
                     /translation="MNFPPVAAGRLPDLLRRMPKAELHMHIEGSLEPELMFALASRNG
                     LALPYPDVASLKKAYVFDNLQDFLNIYHAGTQVLRTGQDFYDMAWAYLQQAAADHVLH
                     TEIFFDTQTHTGHGVDEAVVITSLHRACVDAQTQLGLSAGLILCFLRHLSEAEAFDAL
                     AQAEPHLDKLLGVGLASSEVGHPPEKFARVFAQARKLGLRLVAHAGEEGPPAYIWSAL
                     DALKVERIDHGVQASRDPALMARLVRERIPLTVCPLSNLKLRVFPSLAQHNLGQMLDA
                     GIVATVNSDDPAYFGGYVNTNFTQTFEALELTARHAYQLAFNSFEASFMQPSAKQACQ
                     DRLTEFFETYQ"
     gene            5658..6281
                     /locus_tag="PLB25_08705"
     CDS             5658..6281
                     /locus_tag="PLB25_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HOE41692.1"
                     /translation="MNHLKLTYFDVHGGRAEAIRLALHLGGVAFEDFRFPFPKFADVR
                     KTTPFGQVPTLMVDDVQVTQCDSILRYAGRLANLYPADPFQALLCDEVMYVVEEASVK
                     LGPTFHMTGDAQKAARLALVNGSMPQYLAWLQKQLQAHGGDYFADQRLTVADLKVFVD
                     VRALNSGWLDHVPVDLVQTVAPLLNVHLQRIAQNPAVLAYYAQFAPK"
     gene            complement(6311..7072)
                     /locus_tag="PLB25_08710"
     CDS             complement(6311..7072)
                     /locus_tag="PLB25_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013587614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family protein"
                     /protein_id="HOE41693.1"
                     /translation="MPLLRGWTAVQRTAANNVRLGVTLTFVAGATNAGGFLAVGQYTS
                     HMTGIVSSMADNLVMGQAYLALAGLASLLAFIGGAMTTAWMVNWALRRKLQSAYGRPL
                     LLEAALLLLFGLFGSGIRFLAVLFVPLTVLVLCFIMGLQNAVITKISRAEIRTTHITG
                     LVTDLGIELGKLFYINRLTREQMVVGNRKKLRLLGKLIGSFFVGGLLGALGFKTIGFF
                     TTVPLSVLLLLLVWRPVLNDARNWIGPPHPGQDQD"
     gene            complement(7097..>7536)
                     /locus_tag="PLB25_08715"
     CDS             complement(7097..>7536)
                     /locus_tag="PLB25_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOE41694.1"
                     /translation="PPAQTESALAAIENVVDQKPGGESIAAAISIDDFARIDLRIAKI
                     VQCQAVAGSTKLLQLTLDVGEVDDAGLPKTRTVFSGIASSYQPGQLTGKLTVLVANLA
                     PRKMKFGISEGMVLAAGHADEKAHPGIYLLEPFAGAMPGMRLR"
BASE COUNT         1523 a         2223 c         2350 g         1440 t
ORIGIN      
        1 caaccagctc gccgcgcttg aactcaacgc ctgagcggtg gcggccagga cgggttggca
       61 gcaggaagcg ctcaattgcc aatggcttgc ttgaagctgg actcctcagt tcgccgcttg
      121 tttcaaacgg taagtccata tcagcattta ctttttttgc ttcgatgggt tcacctgttg
      181 ggtcacagcg ctgcgcaccc gattgaacca ctggcaacgc gactgggggc gtcactcctg
      241 cctcgtctgc caggacgaca tgcgctgaag tagctgagcg tcgttgtcgg cgctcgcagc
      301 ttgcgacatg caaggcgcag ccgtgccaca ggctggccac ttcaatccgt ccaagcttgc
      361 gcaggcaggt ttagagctgc ggctttcgcc ctgcccagcc gcttcgccat tggaccactc
      421 gggtatgctc aactggggaa tcaaggttga tgccaatgca agcaaggcgg cttcaaaatg
      481 gcctgcaagc ctgagcgact gcatggcagc caggcatgcg ggttttccct tgaaaacctg
      541 ctgtttgcca caagttgcgg tgtaacttgg agaaattgcc atgcgaattg aaaaactgac
      601 gaccaaattt caggaggccc tggctgacgc gcaatccctg gcgctggaaa aagaccatgc
      661 ctacatcgaa ccagcccacc tgctggtggc catgctcaag caagacgacg gtccaaaagc
      721 cctgttgcaa cgcgccggtg tgaacgtggc ggggttgttg aatgcggccg aggcggcggt
      781 gaagaagctg ccgcaagtca ccggccagga ccaggtgcaa ggcgggccgg agctgggcaa
      841 gctgctgcag gcgaccgaaa aggaagccct caagcgcggc gaccagtttg tcgctggcga
      901 actgctgttg ctggcactgg ccgatgccaa gggatcgctc ggcgatgcgg cacgggccaa
      961 cggcctgacg cgcaagagcc tggagtccgc catcgaagcg gtgcgcggtg gccagagcgt
     1021 ggacagcgcc aatgccgaag accagcgtga atcgctgaaa aaatactgcg ttgacctgac
     1081 cgagcgcgcc cgcatgggca agcttgaccc ggtgattggc cgcgacgatg aaattcgccg
     1141 cgccattcag gtgctgcaac gccgcaccaa aaacaacccg gttctgattg gcgagcccgg
     1201 cgtaggcaag accgccattg tggaaggcct ggcgcagcgc atcgtggctg gcgaggtgcc
     1261 cgagtcgctc aagggcaaac gggtgctgag cctggacatg gcatcgctgc tggccggtgc
     1321 caaatttcgc ggcgaattcg aagaacgcct gaaaaacgtg ctcaaagacc tggccaagga
     1381 cgaaggccag accatcgtgt tcatcgatga actgcacacc atggtcgggg ctggcaaggc
     1441 cgaaggtgcg atggacgcgg gcaacatgct caaacccgcc ctggcgcgcg gtgaactcca
     1501 ctgcgtgggt gccaccacgc tcgacgagta ccgcaagtac attgagaagg acgcggcgct
     1561 ggaacgtcgt ttccagaaaa ttctggtcgg cgagccgtcg gtggaggcga ccattgccat
     1621 tttgcgcggt ctcaaggagc gctacgaaaa gcaccacaac gtcgaaatca ccgacccggc
     1681 catcgtcgcc gcagccgaat tgagccaccg ctacatcacc gaccgctttc tgccggacaa
     1741 ggcgatcgac ctgatcgacg aggcagccag caagatcaag atcgagatgg attcgaagcc
     1801 cgaggtgatg gacaagcttg accgccgcct gatccaattg cagattgagc gcgaggcggt
     1861 gaagaaagaa aaagacgagg catcgctcaa gcgccttgaa ctcatcaccg acgagatcaa
     1921 atcgctgcaa aaggaaattg ccaaccttga tgaagtctgg cgggctgaaa aagccagcgc
     1981 gcagggcagt ggcgacctgc gtgaacagat cgacttgctc aaattcaaga tcgaggaact
     2041 cacccgcaag ggcgatttca acaaggtggc cgaattgcag tacggcaaac tgcctgaact
     2101 ggaaaagcgc ctgaaagagg cccaggcgaa ggaatccact cgcggcgaag gagcggcacc
     2161 gcgcttgctg cgaacccagg tcggggccga agaaatcgcc gaggtggtga gccgttccac
     2221 cggcattccg gtgtccaaga tgatgcaagg cgagcgcgac aagctgttgc agatggaagt
     2281 caagctgcac cagcgcgtgg tcggccagga cgaggccatc gctgcggtgg ccaatgccat
     2341 tcgccggtcc cgctcggggc tgtcagaccc gaaccgcccg accggctcgt tcttgttcct
     2401 cgggcccacc ggggtcggca agaccgagct gtgcaaggcg ctggcgggtt tcatgtttga
     2461 ctccgaagat catctgattc gcatcgacat gagcgagttc atggaaaagc acagcgtggc
     2521 gcggctgatt ggtgcgcccc ccggctatgt cggttacgaa gagggtggcc acctgaccga
     2581 ggcggtgcgg cgcaagccct acagcgtgct gctgctcgac gaggttgaaa aagctcaccc
     2641 cgatgtcttc aatgtgctgc tgcaggtgct tgacgatggc cgtctgaccg atggtcacgg
     2701 ccgcaccgtg gacttcaaga acacggtgat cgtgatgacc agcaatatcg gctcgcaaat
     2761 gatccagaac atgctcgggc aagactacga agacgtgaaa gacgcggtta ccgccgagct
     2821 gaagaaccac tttcggcccg agtttctcaa ccgcatcgat gagacggtgg tgttccattc
     2881 gctcgatgcc agccacattg ccgatattgc caagatccag ttggcggtgc tgcaggcgcg
     2941 cctggcgcac atggacctgg ggctctcggt cagcaacgct gccgtggccg agttggccaa
     3001 ggtcgggttc gacccggtgt ttggcgcaag acccctcaag cgtgcgattc aacagcgcct
     3061 tgaaaacccg ctgtccaagc tgctgctcga aggcaagttc gccccgaagt ccaccattca
     3121 cgtcgaggtg gacaccattc gggccccagg ccaattcaac tttgtttgag ggtgttttag
     3181 ccttctagcc caagtggcat aagcgcaggc agctactgat ataggagcaa tccggtgtcg
     3241 gtggtggcct gcgcagtgca aaccattggc cgggttggcg tgtcgcttgc agcggctgtg
     3301 cggccgccac gccagtttgc tgcgtccatc cggttgggct tgcctggttt ggggcaacgg
     3361 ggtcgatctg ccttgctgca cccggactgc gataattggc gcatgcaaaa agccctttcc
     3421 caaccccttt tgagtctctg gcggccgatt ctggcgatgc tggtggtgat cgtctggtcc
     3481 aactacctgg tgcagtttcc catcaatgat tggctgacct ggggcgcttt tacctttccg
     3541 gtggcgtttc tggtcactga tctgaccaat cgcgcggtcg gtgcccaagc ggcgcggcgg
     3601 gttgcctgga ccggctttgc catggctgtg ctggtgtcgc tggcgttgac cccgtggcgc
     3661 attgcggtgg cctctggtgt ggccttcatt gcgggccaac tgctcgacat cgtggcgttc
     3721 aaccggctgc gtgcctcgtc atggtggaaa gccccgctga tcggctcggt ggtggcctcg
     3781 gtggtcgata ccggcatttt ctttttcctc gcctttgccg ggtctgacct cgattggcgc
     3841 atgttggcca ctggtgacct ggcggtcaag tggctgatgg cggctctgct attggcacct
     3901 taccgggtca tgctgccgcg cctgcagctt tgggtgccgg tgcactgatt gccagggccg
     3961 ccacaactgt ggcaggtgtc gcgctgccgt agctgtcact gccgatcact gataggtttc
     4021 aaaaaactcg gtcaagcggt cctggcaggc ctgcttggcg gacggctgca taaagctcgc
     4081 ctcaaaactg ttgaatgcca attggtaggc gtgccgggcg gtgagttcca gcgcttcgaa
     4141 ggtctgggtg aagttggtgt tgacatagcc accaaaatac gccgggtcgt ccgagttgac
     4201 ggtggcgaca atgcccgcgt cgagcatctg gccaaggttg tgctgcgcca ggctggggaa
     4261 cacccgcagt ttcaggttgg atagcgggca gacggtcagc gggatgcgct cgcgtaccag
     4321 ccgcgccatc agagccgggt cgcggctggc ttgcacgcca tggtcaatgc gttcaacctt
     4381 gagcgcatca agcgcgctcc agatgtaggc tggcggtcct tcttcaccgg catgggcaac
     4441 cagccgcagc ccgagcttgc gggcctgggc aaagacccgg gcaaatttct ccggcggatg
     4501 cccgacttcg ctcgaagcca ggccaacgcc tagcagtttg tccagatggg gctcggcttg
     4561 cgccagcgcg tcaaaggctt cggcctcgct cagatggcgc aaaaagcaca ggatcaggcc
     4621 cgcgctcagg cccaactggg tctgggcatc cacacaggcc cggtgcaagc tggtgatcac
     4681 caccgcttca tcaacgccgt gtccggtatg ggtttgcgtg tcaaagaaga tttcggtgtg
     4741 cagcacatga tctgccgctg cctgctgcag gtaggcccag gccatgtcgt agaagtcctg
     4801 cccggtgcgc agcacctgcg taccggcgtg gtagatgttc aaaaagtctt gcaggttgtc
     4861 aaacacatag gcttttttca ggctggcaac atccgggtag ggcagggcca gaccattgcg
     4921 actggccagc gcaaacatca gctcgggctc cagcgagcct tcaatgtgca tgtgcaactc
     4981 ggctttgggc atgcggcgca gcaaatcggg cagtcggccc gcggcgacag gaggaaaatt
     5041 catggtgcga tggttaaaaa atactataaa ataaatagct ctcagcgcat acagtgtaag
     5101 ggctagagcc ctatttcttc acttttttgc ggctgaccca gtggtgctga tcgccgtagc
     5161 ggcaaaaaat acagaaggca ccagcagcac gcaagaataa cctcaatgcc agccggtatg
     5221 cgcatgacgg catcgcctga ccttgcgcca tttaccacgg catgcgccac agcatgcccg
     5281 gtgcccattg atcgccgaac ctgtcagcgc tgcgcttgcc gcaaattggc tggcaatgca
     5341 gcaaggcgat gagccggggt gtgtgccggg tgtgctggat gtgtgccggg taatgtggtg
     5401 ggtagtgccg taaggcaatg ccgacggcgg tggtgcaccg ttgcgaccac ccgaaccgcc
     5461 caggcgcttc tcggctcgtt gccggtctgg cgaccgcttc aatttgatag ctgctcaccc
     5521 ttattccaaa agggctggaa gtccaattct tttgtggcgc ggcagccaaa cgcggtcccg
     5581 cctagaatcc gccgaacctc ctgcgcggca ttgccgccag agcctttgcc ccctcaacac
     5641 accaccagga aaccaccatg aatcacctca aactcactta tttcgatgtc cacggcgggc
     5701 gcgccgaggc gatccggctg gcgctgcatc tggggggcgt ggcgtttgaa gatttccgct
     5761 ttccttttcc caagtttgcc gacgttcgca agaccacacc gtttggccaa gtgcccacgc
     5821 tgatggtgga cgacgtgcag gtgacgcagt gcgactccat cctgcgctat gcgggcaggc
     5881 tggccaacct ttacccggcg gatccgtttc aggcactgct gtgcgatgag gtgatgtatg
     5941 tggtggaaga agccagcgtc aagcttgggc ccaccttcca catgaccggt gacgcgcaga
     6001 aagcagcgcg cctggcgctg gtgaacggct ccatgccaca gtacctggcc tggctgcaaa
     6061 agcagttgca agcgcatggc ggcgattact ttgccgacca gcgcctgacc gtggcggacc
     6121 tgaaggtgtt tgtcgatgtg cgcgccctga actcaggatg gcttgaccat gtgccggtcg
     6181 acttggtgca aacagtggcc ccgctgttga acgtgcacct gcagcgcatc gcccaaaacc
     6241 cggcggtgct ggcgtattac gcccaatttg cccccaagta actcggcgcg gcgtgactgg
     6301 ccaggtgctc tcagtcttga tcttgtccgg ggtggggtgg cccaatccag ttgcgggcat
     6361 cgttgagcac cgggcgccag accagcagca gcaacaagac cgacagcggc accgtggtga
     6421 aaaagccaat ggtcttgaag cccagggcac cgagcagacc gccgacaaag aagctgccga
     6481 tcagcttgcc aagcagcctg agctttttgc ggttgccgac caccatctgt tcacgcgtca
     6541 ggcgattgat gtagaacagc ttgcccagct caatccccag gtcggtcacc aggccggtga
     6601 tgtgggtggt gcgaatctcg gcacgggata ttttggtgat gacggcattt tgcagcccca
     6661 tgatgaagca caacaccagc accgtcagcg gcacaaacag cacggccaag aaacggatgc
     6721 ccgagccaaa cagcccaaac agcagcagca gggcggcttc gagcagtagt ggtcggccat
     6781 aagcgctttg cagcttgcgc cgcagcgccc agttgaccat ccatgcggtg gtcatggcac
     6841 cgccaataaa ggccagcagc gaagccagcc cggccagcgc caggtaggcc tggcccatca
     6901 ccaggttgtc ggccatcgac gacacgatgc ctgtcatgtg cgaggtgtac tgcccaaccg
     6961 ccagaaagcc gccggcattg gtggccccgg caacaaaggt cagggtgaca ccgagccgga
     7021 cattgttggc ggcggtgcgc tgaactgccg tccagccgcg cagcagggga atcatcccgt
     7081 gctcactgca agcgcctcaa cgtaagcgca tgcccggcat ggcaccggca aagggttcga
     7141 gcagatagat gccgggatgg gctttttcat cggcatggcc agcggccagc accatgcctt
     7201 cgctgatgcc aaacttcatc ttgcgcgggg ccaggttagc caccagcacg gtcagcttgc
     7261 ctgtcagttg ccccggctgg taactgctgg caatgcctga gaacaccgtg cgggtcttgg
     7321 gcaggcctgc gtcatcgacc tcaccgacat cgagtgtgag ttgcagcagt ttggtggaac
     7381 ccgctaccgc ttggcactgc acgattttgg caatgcgcag gtcgattctg gcgaagtcgt
     7441 caatgctgat ggcagcggct atggactcgc cgccgggctt ctggtccact acattttcaa
     7501 tagctgccaa cgcagactca gtctgggctg gcggct
//
LOCUS       DAOSLL010000159         7459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9090_length_7459_cov_4.787952, whole genome shotgun sequence.
ACCESSION   DAOSLL010000159 DAOSLL010000000
VERSION     DAOSLL010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7459
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9090_length_7459_cov_4.787952"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(477..1079)
                     /locus_tag="PLB25_08720"
     CDS             complement(477..1079)
                     /locus_tag="PLB25_08720"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HOE41695.1"
                     /translation="MGQFVYLASQSPRRQQLLAQLGVAFELLLPGDDEDAEALEALLP
                     RESPLHYVQRVTQLKLDAALLRRKRRGLPDAPVLCADTTVALRRVILGKPVDAADAAR
                     MLQQLAGNTHTVLTAVAVAAERKRLQAVSRSQVTFAPMTAAQIDAYAASNEPIGKAGA
                     YGVQGRAAMHISKISGSYSGIMGLPLFETAQLLLGIGFKI"
     gene            complement(1088..1555)
                     /gene="rlmH"
                     /locus_tag="PLB25_08725"
     CDS             complement(1088..1555)
                     /gene="rlmH"
                     /locus_tag="PLB25_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HOE41696.1"
                     /translation="MRLLIVAVGQRVPDWAQTAWDDYAKRFPFEIKVELKAVKTEPRA
                     SRSLETLLSAERARIEAAIPRACRVVALDERGTALTTMALAGKLRDWQLSGDDVALVI
                     GGPDGLEPAFRQAAHERIRLSDLTLAHAFARVLLIEQLYRAWSINANHPYHRE"
     gene            complement(1558..2319)
                     /gene="rsfS"
                     /locus_tag="PLB25_08730"
     CDS             complement(1558..2319)
                     /gene="rsfS"
                     /locus_tag="PLB25_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HOE41697.1"
                     /translation="MATKATGKKDIQKLQRAIVDGLEDVKAQEIVVFDTEHLSSLFER
                     VIIASGSSNRQTRALAASVMDAVREAGFGKPRLEGESNGEWIIVDCGQAVVHVMQPTF
                     RTYYNLEELWGDKPVRLKLGAAKPVAAIDTKAAPVAPVSARPPVSLRRSSAAKKGVEE
                     AFPSRAQLKKVSDAKSVAAKKIACSAHAKRAAGQFDAEGAARPKVRTLVVNAPAKPAA
                     KRALASKAASRSTAKPASPTATRSAPARKTTPRKA"
     gene            complement(2400..3014)
                     /gene="nadD"
                     /locus_tag="PLB25_08735"
     CDS             complement(2400..3014)
                     /gene="nadD"
                     /locus_tag="PLB25_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HOE41698.1"
                     /translation="MPEKLVPRRVGVMGGAFDPPHLAHSALAAVACSQLQLDELRVIP
                     TGQAWHKPQALSAARHRLAMAQLAFADVPQARVDARETRRAGPSYSIDTLNELRAESP
                     GSQLFLIIGADQAHALTSWKAWQEILQCATICVADRADSIRAGAQFDAEVANPARFLH
                     LAMPAMAISATEIRARISANLSVHALVCEPVARYIAAHHLYQTV"
     gene            complement(3014..3946)
                     /gene="hemF"
                     /locus_tag="PLB25_08740"
     CDS             complement(3014..3946)
                     /gene="hemF"
                     /locus_tag="PLB25_08740"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HOE41699.1"
                     /translation="MSEAVPPGGAAALVSQYMRGLQARITTAIGALDGTPFVTDAWQK
                     PPGDTLQGHGITQILEAGPVFERAGCGFSQVSGPTLPPSATQHRPELAGAPFQAMGVS
                     LVFHPRNPYVPTVHMNVRMISATGPDGVPVCWFGGGMDLTPFYGFEEDAAHFHQCCKA
                     ALQPFGDDKYTRFKVWCDSYFYLRHRDEQRGIGGVFFDDFSELGFDQSLAMLQSVGDA
                     FLGAYLPIVERRKDLAYGQRERDFQLYRRGRYVEFNLVWDRGTHFGLQSGGRTESILL
                     SMPPLVSWAYRQVPEAGTPEAALYSKFLTRRDWV"
     gene            complement(3943..5244)
                     /gene="purD"
                     /locus_tag="PLB25_08745"
     CDS             complement(3943..5244)
                     /gene="purD"
                     /locus_tag="PLB25_08745"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HOE41700.1"
                     /translation="MKILVIGSGGREHALAWKIAQSPKAHKIYLAPGNGGTALDPRFE
                     NVNITDVQELRAWAAAKKIVLTVVGPEGPLAAGVVDEFRKHGLRVFGPSQAAAQLESS
                     KAFSKAFMKRHGIPTADFETFTDAVAAHAYIDQKGAPIVVKADGLAAGKGVVVAMNLT
                     EAHAAVDFMLQGNSLGVVHNDGGARVVIEEFLQGEEASFIVMVDGKNVLPLATSQDHK
                     RLLNGDLGPNTGGMGAYSPTPVVTPDVHARAMRDIILPTIRGMEKDGIPYTGFLYAGL
                     MIDAVGKPKTLEFNCRMGDPETQPMMMRLKSDLVDVMLAATEPGPHGKLDLVKLQWDR
                     RTALGVVLAAHGYPSQPRKGDIIMGLPKEHEDACVFHAGTSLANGQVITTGGRVLCVT
                     VLADNARLAQQRAYEVAAGIRFDGMQYRTDIGHRAIKGSAA"
     gene            complement(5241..5975)
                     /locus_tag="PLB25_08750"
     CDS             complement(5241..5975)
                     /locus_tag="PLB25_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE41701.1"
                     /translation="MAGHSKWANIQHRKGRQDEKRQRIWTRVMREIMVAARMGGGDLT
                     TNPRLRLAIEKAKASNMASDTVKYGIAKATGSLEGIVYEEIRYEGYGIGGAAIIVDTM
                     TDNKVRTVAEVRHAFSKYGGNMGTEGSVAFQFRHCGQLIFAPGASEDQVLEIALDAGA
                     DDVIADADGAIEVITPVASFEAVKNALVAAGFAPDDAQVTMRPENPIDMSGEEALRMQ
                     KLLDAIEDLDDVQEVYHNAELDTDGV"
     gene            6034..>7459
                     /locus_tag="PLB25_08755"
     CDS             6034..>7459
                     /locus_tag="PLB25_08755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOE41702.1"
                     /translation="MPEPILIAQHGQTQCFLLPDKANRHGLITGATGTGKTITLQTMA
                     EGFSRLGVPVFMADIKGDLTGISQTGVATPKLLKVLTERALPASVFEAFPTTLWDVFG
                     KQGHPVRATVSDLGPLLLSRMLNLNETQAGVLQLVFKIADDDGLLLLDMKDLRAMCQY
                     VGDNASSFTTEYGNVSAASIGAIQRGLMQIEQQGGNAFFGEPMLNIADFMQTDGAGRG
                     VVNVLAADQLMNSPRLYSTFLLWLLSELFETLPEVGDLEQPKLVFFFDEAHLLFADAP
                     KVLVERIELVVRLVRSKGVGVYFVTQNPLDIPDSVLAQLGNRVQHALRAFTPRDQKAV
                     KATAETMRPKAGLDIGAAITELGVGEALVSFLDEKGRPTETERVFVLPPASQIGPISA
                     QQRQTLLDESIVAGVYEKLLDRDSAFEKLKGHAAVRAEQANSRAAQAPGKAPGTDTAA
                     APAAPAGSGGGMFDSISSGLGSLLG"
BASE COUNT         1429 a         2358 c         2203 g         1469 t
ORIGIN      
        1 aaatgttcga cggtagcctg gccccggttg atttcctgaa aaacctgaat cgcgagatgg
       61 tttgcaccgt gattcaaagc tgcgcaagtt ctgccgggtc gcgtgctgat gcgcttttgg
      121 cattggaaaa acgtatctga tgccattgct gcatcatccc ggtccagccg accttgccaa
      181 cgcaacgcaa ggcaaggcaa ggcaaggcaa gatggcaacg acatgaatgg cttggagacg
      241 gcaaaagcca accccttttt tcgcatccag accgttgcga catttggcgc gaagccgcgg
      301 accatgcgac agcacggcaa ggcaaagcaa caggtcggat tgatatcgaa atggaaaggg
      361 catcaaaggg cgaatcaatc agcccagcgc catgctgtgc cgcggcttga ccggcctgcg
      421 ccatgaatca gcttgaaata aagtgtccgg acagcaccag cttgccgatc aaaacgctag
      481 attttgaagc caatccccag gagaagctgg gccgtctcaa acagtggcag gcccatgatg
      541 ccgctatagc tgccgctgat cttgctgata tgcattgccg ccctgccctg caccccgtag
      601 gcaccggctt tgccgatggg ctcgttactg gccgcataag catcgatctg ggcagcagtc
      661 atcggcgcga acgtcacctg cgagcgcgac acggcctgca gccgtttgcg ctcggcagcc
      721 accgccacag cggtcagcac ggtgtgggtg ttgccagcca actgctgcaa catgcgcgcg
      781 gcatctgcgg catcaaccgg tttacccaaa atgacgcgcc gcaatgccac cgtggtgtcc
      841 gcgcacagca ccggcgcgtc cggcaggcca cgccgtttgc gccgcagcag ggccgcatcg
      901 agcttgagct gggtcacccg ctgcacatag tgcaggggtg attcgcgcgg cagcagcgcc
      961 tccagcgcct cggcgtcttc atcgtcaccc ggcagcagca gctcaaaggc cacacccaac
     1021 tgcgccagca actgctggcg gcgcgggctc tgtgaagcca gatagacaaa ttgacccatg
     1081 gccagcccta ttcgcggtgg tatgggtgat tggcattgat cgaccaggcg cggtaaagct
     1141 gctcaatcag cagcacccgc gcaaaggcgt gcgccagggt caggtccgac aggcgaatgc
     1201 gctcgtgcgc ggcctggcga aaggctggct ccaggccatc gggcccgcca atcaccagcg
     1261 ccacatcgtc acctgacaat tgccagtcgc gcagcttgcc agccaatgcc atggtggtca
     1321 gcgcggtgcc acgctcatcg agcgccacca cccggcaagc cctgggaatg gcggcttcga
     1381 tgcgggcgcg ctcagcggac aacagagttt cgagcgacct ggaagcgcgt ggctcggttt
     1441 tgacggcctt gagctcgacc ttgatctcaa acgggaaccg cttggcgtag tcgtcccagg
     1501 cggtttgtgc ccagtccggc acgcgctggc caaccgccac aatcagcagg cgcacgctca
     1561 ggctttgcgc ggcgtggtct tcctggcagg agcagaccgg gtcgctgtcg ggctggcagg
     1621 ctttgccgtc gaacgggagg ccgccttgga tgccagcgct ctttttgccg ccggtttggc
     1681 cggggcattg accaccagcg tgcggacctt cggcctggct gcgccttctg catcaaattg
     1741 gcctgcagcc cttttggcat gtgcgctgca agctatcttt ttcgcagcaa ctgatttcgc
     1801 atccgagacc tttttgagct gtgccctgga tggaaacgcc tcttcaacgc cctttttggc
     1861 ggcactggaa cgccgcaggc taaccggtgg ccgggcgctc accggggcga ccggggcggc
     1921 cttggtgtcg atggcagcaa ccggcttggc cgcgccgagc ttgagtcgca ccggcttgtc
     1981 gccccacagt tcctccaggt tgtaataggt ccggaaggtg ggctgcatca catgcaccac
     2041 ggcctgcccg caatcgacaa tgatccattc accattgctc tcaccttcga ggcgcggctt
     2101 gccaaagccg gcctcgcgca ccgcgtccat cacactggcc gctagcgccc gggtttgccg
     2161 gttggagctg cccgaggcga tgatcacccg ctcaaacaag gaagagagat gttccgtatc
     2221 aaacaccaca atctcctgcg ccttgacatc ttcgagtcca tccacaatgg cacgctgaag
     2281 tttttgaatg tcttttttgc cggtcgcttt ggttgcaatc gctttggtgg cagtcgcttt
     2341 ggctgccagc ttgggggcga ccggccgggt ttgggctttg gtggtggttt tggtagtcat
     2401 caaacagttt ggtagaggtg gtgggcggca atataacgtg caaccggctc gcacaccagt
     2461 gcatgcacgc tcaggttggc agaaattctt gcccggatct cggtggcgct gatggccatg
     2521 gcaggcattg ccaggtgcaa aaaccgcgca ggattcgcaa cttcagcatc aaattgggct
     2581 ccagccctta tggaatctgc gcgatcagct acacaaattg tagcacattg aaggatctcc
     2641 tgccatgcct tccagcttgt caaggcatgc gcctggtcag cgccaatgat caggaacagt
     2701 tggctgcccg gcgactcggc cctgagttca ttgagcgtgt cgatgctgta gctcggccct
     2761 gcgcgcctgg tttcacgcgc atccacccgc gcctggggca catcggcaaa ggccagttgc
     2821 gccatcgcca ggcgatgccg ggccgcgctg agggcttgcg gcttgtgcca ggcctgcccg
     2881 gtcgggataa cacgcaactc gtcaagctgc aattgggaac acgccactgc cgccagggcc
     2941 gaatgcgcca gatgcggtgg atcgaaggcc ccgcccatca cacccacccg cctgggcacc
     3001 agcttttcgg gcatcaaacc cagtcccggc gcgtcagaaa cttgctgtac agcgccgctt
     3061 cgggtgtgcc agcttcaggc acctgtcggt atgcccagct taccagcggc ggcatcgaca
     3121 acaaaatcga ctcggtgcgg ccacccgact gcaggccaaa atgcgtgccc cggtcccaca
     3181 ccaggttgaa ttcaacatag cggccgcgcc ggtaaagctg aaaatcgcgc tcgcgctggc
     3241 cgtaggcaag atccttgcgc cgttcgacaa tcggcagata cgcgcccaaa aaagcatcgc
     3301 ccaccgactg cagcatggcc agactctggt caaagcccaa ttcggaaaaa tcgtcaaaaa
     3361 acacgccacc gatgccacgc tgctcgtcgc ggtgcctaag gtaaaaatag ctgtcgcacc
     3421 agaccttgaa gcgcgtgtat ttgtcgtcgc caaacggctg caacgcggct ttgcagcatt
     3481 ggtgaaaatg cgccgcgtct tcttcaaagc cgtaaaacgg tgtcaggtcc atgccgccgc
     3541 caaaccagca aaccggcacg ccgtctggcc cggtggccga aatcatgcgc acattcatgt
     3601 gcaccgtcgg cacgtagggg ttgcgtggat gaaacaccag cgatacaccc atggcctgaa
     3661 atggcgcgcc agccaattct ggccggtgtt gcgtcgcaga cggcggcaat gtcgggccgc
     3721 tgacctgtga aaacccgcaa ccggcgcgct caaaaaccgg cccggcttcg agaatctggg
     3781 tgatgccatg gccttgcaag gtatcacccg gtggcttttg ccaggcatcg gtcacaaaag
     3841 gcgtgccatc aagcgcgcca atcgccgtcg tgatacgggc ctgcagccca cgcatatact
     3901 ggcttaccag cgccgctgcg ccgccgggcg gcactgcttc gctcatgccg ccgagccctt
     3961 gatggcgcgg tggccaatgt cggtgcggta ctgcatgcca tcaaagcgga tgcctgcggc
     4021 cacctcatag gcgcgttgct gcgccaggcg ggcgttgtca gccagcaccg tgacgcacag
     4081 cacccgcccg ccggtcgtga tcacctgccc gtttgccagg cttgtgccag catgaaaaac
     4141 gcaggcatcc tcatgttctt tcggcaaacc catgatgatg tcgcccttgc gcggctgcga
     4201 cgggtagccg tgcgccgcca gcaccacgcc cagtgcggtg cgacgatccc attgcagttt
     4261 caccaagtcg agcttgccat ggggccctgg ctcggtggcg gccagcatca catcgaccag
     4321 atcagacttc aggcgcatca tcatcggctg ggtttcgggg tcacccatgc ggcagttgaa
     4381 ttccagcgtt ttgggttttc ccactgcatc aatcatcaaa ccggcgtaca aaaacccggt
     4441 gtacggaatg ccgtcttttt ccatgccacg gatggttggc aagatgatgt cgcgcatggc
     4501 ccgcgcatgc acatcggggg tgaccaccgg tgtcggtgaa taggctccca tgccaccagt
     4561 gttggggccc agatcaccgt tgagcagccg cttgtggtcc tggctggtgg ccagtggcag
     4621 cacgttcttg ccatcgacca tgacgatgaa gctggcctct tcgccctgga gaaactcttc
     4681 aatcaccacg cgtgctccgc cgtcgttgtg caccacgccc aggctgttgc cttgcagcat
     4741 gaagtccacc gccgcatgcg cttcggtcag attcatggcg accaccacgc ccttgccggc
     4801 cgccagacca tcggccttga ccacaatcgg cgcgcccttc tggtcgatat aggcatgcgc
     4861 cgccacggca tcggtgaagg tttcaaagtc ggcggtggga atgccgtggc gcttcatgaa
     4921 ggctttggaa aacgcctttg agctttccag ttgcgccgcc gcctggcttg ggccaaacac
     4981 ccgcagaccg tgcttgcgga attcatcgac cacgcctgcc gccagcggac cttcggggcc
     5041 gaccaccgtc agcacgatct tttttgcagc agcccaggcc cgcagctctt gcacatcggt
     5101 gatgttgacg ttctcgaaac gcgggtccag cgcggtgccg ccgttgcccg gtgccaggta
     5161 aatcttgtgg gccttgggtg actgggcgat cttccaggcc agcgcatgtt cacggccacc
     5221 gctgccaatg acgagtatct tcatacgccg tctgtatcga gttcggcgtt gtgatagacc
     5281 tcctgcacgt cgtcaaggtc ttcaatggcg tcgagcagct tttgcatgcg caaggcttct
     5341 tcgccactca tgtcaatcgg gttttcaggg cgcatcgtca cctgtgcgtc gtccggggca
     5401 aagccagccg cgaccagggc atttttgacc gcttcaaaac tggccaccgg cgtgatcacc
     5461 tcaatcgccc cgtctgcgtc ggcaatcaca tcgtcggccc cggcatcgag cgcaatttcc
     5521 agcacctggt cttcgctggc accgggggcg aaaatgagct ggccgcagtg cctgaactga
     5581 aacgccaccg agccctcggt gcccatgttg ccaccgtact tggaaaaggc gtggcgcacc
     5641 tcggccaccg tgcgcacctt gttgtcagtc atggtatcga cgatgatggc ggccccgcca
     5701 atgccgtaac cttcgtagcg tatttcctcg taaacgatgc cctccagact gccggtggcc
     5761 ttggcgatgc cgtatttgac ggtatcagat gccatgttgg acgccttggc cttttcaatc
     5821 gccaggcgca ggcgcgggtt ggtcgtcaaa tcgccgccgc ccatgcgcgc cgcgaccatg
     5881 atttcgcgca tgacgcgggt ccagatacgc tggcgctttt cgtcctgacg acctttgcga
     5941 tgctgaatat tggcccattt actgtgtcct gccacgggaa atccttcaaa agttcgttac
     6001 cctactggtc tgattgtact ttttgagctt gccatgcctg aacccattct gattgcacaa
     6061 cacggtcaaa cgcaatgttt cttgctgcct gacaaggcca atcgccacgg tttgatcacc
     6121 ggtgccactg gcaccggcaa gaccatcacc ctgcaaacca tggccgaagg gttttcaaga
     6181 ctgggtgtgc cggtcttcat ggccgacatc aaaggcgatc ttaccggcat ttcgcagacc
     6241 ggtgttgcaa caccaaaact gctcaaagtc cttactgagc gtgcgctacc tgcttctgtt
     6301 tttgaagcgt ttccgaccac cttgtgggat gtctttggca aacaggggca tccggtgcgc
     6361 gcgaccgtca gcgacctcgg gccgctgctg ctctcgcgca tgctcaacct caatgagacc
     6421 caggcaggcg tgctgcaact ggtgttcaaa atagccgatg acgacggcct gctgctgctc
     6481 gacatgaaag acctgcgcgc catgtgccaa tacgtgggcg acaacgcgtc gagcttcacc
     6541 accgaatatg gcaacgtcag cgccgccagc attggtgcca ttcaacgcgg cctgatgcaa
     6601 atcgagcaac aaggtggcaa tgcttttttt ggcgagccga tgctcaacat tgccgacttc
     6661 atgcagaccg acggggccgg gcgcggtgtc gtcaatgtgc tggcagccga ccagttgatg
     6721 aattcgccgc gcctctacag cacctttttg ctctggctgc tcagcgagtt gtttgaaaca
     6781 ttgcccgaag tgggcgacct ggagcaaccc aagctggtgt ttttctttga cgaggcgcac
     6841 ctgctgtttg ccgacgcgcc caaggtgctg gtggaacgga ttgaactggt ggtgcggctg
     6901 gtgcggtcca aaggggttgg tgtctatttc gtcacacaaa acccgctcga catccctgac
     6961 tcggtgctgg cccagttggg caaccgggtg caacacgcac tgcgggcatt cacgccacgc
     7021 gatcaaaaag cggtcaaggc caccgccgag acgatgcggc caaaagccgg gcttgacatc
     7081 ggtgcggcca tcaccgaact cggggtgggc gaagcgctgg tgagttttct cgacgaaaaa
     7141 ggccgtccaa ccgaaaccga gcgggtcttt gtgctgccgc ccgccagcca gatcgggccc
     7201 atttctgccc agcagcgcca gaccttgctc gatgagtcga tcgtcgccgg ggtctatgag
     7261 aaactgctcg accgggattc ggcgttcgag aagctcaagg gccatgccgc cgtgcgggcc
     7321 gagcaggcca acagccgggc cgcgcaagcc cccggcaaag cccctggcac cgataccgct
     7381 gcagccccgg cggccccggc tgggtcgggc ggaggcatgt ttgacagcat cagctccggc
     7441 ctgggcagcc tgcttggcg
//
LOCUS       DAOSLL010000160         7458 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9094_length_7458_cov_3.920843, whole genome shotgun sequence.
ACCESSION   DAOSLL010000160 DAOSLL010000000
VERSION     DAOSLL010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7458)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7458)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7458
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9094_length_7458_cov_3.920843"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="PLB25_08760"
     CDS             complement(<1..1214)
                     /locus_tag="PLB25_08760"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009341 - beta-galactosidase complex
                     [Evidence IEA];
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HOE41703.1"
                     /translation="MKRRDGFLLLLALCPLVLLLCVWWQLRLPVAPFFLAPMMDLDGC
                     LLARQPTPVAGHAPWLNRCSGPNASAAHLVESTLQALQPDQARAHSWQLGYTLKVSLL
                     ALLQPDQNNWRVNLEVIDKLVQTIQDNPRPLILYLFSTHFGVDAPIEPVLAQNPDNLG
                     QTPAGPLPIDRYYGQAVYPWSVARTDNDITRYRAQVIEALLHKLCQLPANERSRIKGV
                     TLLGEVHHLFPDFESGMGFDKPYSVTDYSATSVADFRQYLRARYPGIEALNQQLGSQY
                     ASFDSIMPPAKDIRRQPLARFEEHLDAYADGHLPISGWVHVQGAPAVVKVYLNGAWVA
                     DAPVHLGRQDVRAAHPEFSTADVGWRHDLDFSKLAPGVYRIDLTLSQPGHKLLNLGTR
                     AISVMDKQQSAPL"
     gene            complement(1211..5068)
                     /locus_tag="PLB25_08765"
     CDS             complement(1211..5068)
                     /locus_tag="PLB25_08765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE41704.1"
                     /translation="MPNDNSLPHVYQRTVSADDRHDSLSVISNLIKPGQTVLDLGMGV
                     GGLGQYLSQQFEVLADGVTLNQAEADIASQWYRQTRVADLDSANLSELFDGQRYDCIV
                     CADVLEHLKAPQNLLAQCKNLLEPGGRLLTSVPNAGYCGLVAELIQGEFRYRPEGLLD
                     STHLRFFTRASLLRFFAENGWAVQATQVTQRSLPTSEFKVAFDSLPPMLARHLLAMPD
                     AQTYQFISVVQPSGSGSTALPAPDQTEAATAPPPQPAEALFSAEVYLARQGRFDESDK
                     LVATGVIGNPRQNLVFDIPASEVAYTSIRLDPADRSGFFKLYHIRITLPDGDVVWQWH
                     AAQDALATLIAQPRQQIMMAPPLVIADNALLLLHGDDPWFELPVAPHQLNQISSQGAR
                     LEVSSGWPMSADYLQASAEFTAQEVIRQQSLATMQWQLDDRKLALTQTEAALQQTETT
                     RQQTQDKLQATSLALQAFRDEKVRLISQLRAAQLEGSTLAESLAQLVQHLEGIEQSTM
                     FRATRPLVNLKMKLDLLTGKSKRITAMQKPNNKAQPRAVTQHPVDIVVPVYRGLEDTR
                     RCLESVLAAPCQAAWRLIVINDCSPEPELADWLRELAGRDTRVQLFENPENLGFVGTV
                     NRGMALSAEHDVLLLNSDTEVANNWLDRIVQAAYSAPKVASVTPFSNNATICSYPVFC
                     KPNDLPSGHNTASLDALFASHLAGQTVPVPTGVGFCMYIRRQCLQQVGLFDTENFGKG
                     YGEENDFCVRAQAAGWVNLHALDTFVRHAGGISFGYSKSEREMQAMQTLRRLHPRYEA
                     DVMNFVARDPARTARLTLDIVRITAAGHPVILNVIHNREGGTLRHIQEVAEKLGQQAI
                     FLRLTPAPGGVILRLEGEHEAFALHFGMPQEQSGLVNALRLLQVGHIHFHHLLGHEAS
                     IVDLPGKLGVSHDFTAHDFFSYCPQISLTDHTDRYCGEQGLYQCRQCLQRHPAPGGVG
                     IEQWRDQHAQLINEARYVIAPSQDTALRLARFAPNANVLVVPHSELDTLVPSYPEPKP
                     ARLDRQKCLKIAVLGALSKIKGADVLEEVATLAAQRGLLIDFHLIGYAYRSLRTQPKA
                     RLTVHGAYDDKDLMKLLQWLQPDAIWFPAVWPETYSYTLSASLDSGLPIIAPDIGAFA
                     ERLQNRPWTWQCDWQQTPGQWTDFFENIRDHHFCSGIGPASASQIPAREWSNGQTFSY
                     RESYLQSLPRPSEPNAQDIKNIQQQLTPNLHWLDVNNTPGNILKSSTLRTIARLRATA
                     AFSPLARLVPTHLQRRVKSWLGK"
     gene            5179..6162
                     /locus_tag="PLB25_08770"
     CDS             5179..6162
                     /locus_tag="PLB25_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011902149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HOE41705.1"
                     /translation="MTTHVAAAQGALKIFVAGHRGMVGSAIVRTLLASGVPQTSIVTR
                     THAELDLCNQAAVQAFFATERPTQVYLAAAKVGGIYANNTFPADFIYDNLMVQANVIH
                     AAHTADVQKLLFLGSSCIYPRMAAQPMTESALLSGALEPTNEPYAVAKIAGIKMCESY
                     NRQFGRDYRSVMPTNLYGPGDNYHPQNSHVIPALIRRFHEARVAGAPEVAIWGTGSPR
                     REFLYVDDMASASVFVMNLPTLTYQTHTQPMQSHINVGFGSDITIDELSHAVGQAVGY
                     LGRISFDATKPDGAPRKWMDSTRLNSLGWQPKVDLVSGLGMAYRAYVQSAK"
     gene            6234..7382
                     /gene="gmd"
                     /locus_tag="PLB25_08775"
     CDS             6234..7382
                     /gene="gmd"
                     /locus_tag="PLB25_08775"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HOE41706.1"
                     /translation="MTTTTSTTAFTPKVALITGITGQDGSYLAEFLLEKGYQVHGIKR
                     RASSFNTERVDHIYQDPHIDNARFKLHYGDLSDTSNLTRILQEVQPDEVYNLGAQSHV
                     AVSFESPEYTADVDGMGTLRLLEAIRILGLEKKTRFYQASTSELFGLVQESPQRETTP
                     FYPRSPYAVAKLYAYWITVNYREAYGMYACNGILFNHESPRRGETFVTRKITRGLANI
                     SQGLEQCLYMGNIDALRDWGHAKDYVRMQWLMLQQDEPCDFVIATGVQYSVRQFIEKA
                     TAALGMQLRWEGEGVAEVAYWLNPTDAARMAHGVDAPIIQVDPRYYRPTEVQTLLGDP
                     TKAREKLGWVPEITLDQLVTEMVTSDLQKARQHALLKANGYLVNVSVE"
BASE COUNT         1577 a         2240 c         2182 g         1459 t
ORIGIN      
        1 agcggggcgc tctgctgctt gtccatcaca gaaatggcgc gggtgcccag gttgagcagc
       61 ttgtgcccag gctgcgacaa cgtcaagtcg atgcggtaaa cgccgggtgc cagcttgctg
      121 aaatccagat catgccgcca gccaacatcg gccgtgctga attcggggtg tgctgcgcgc
      181 acgtcctgac gacccagatg caccggagca tctgccaccc atgcgccatt cagatatacc
      241 ttgaccacag ccggtgcgcc ctgcacatga acccagccac tgatcggcag gtgcccgtcg
      301 gcataagcat caaggtgctc ctcaaagcgc gcaagcggct ggcggcggat gtctttggcc
      361 ggcggcatga tgctgtcaaa gcttgcgtac tggctgccaa gttgctggtt gagcgcctca
      421 atgccggggt accgcgcccg caaatactgg cgaaagtcgg ccactgacgt ggcgctgtag
      481 tcggtcacgc tgtagggctt gtcaaacccc atgccggact caaagtcggg aaacaaatgg
      541 tgaacctcac ccaacagcgt cacccccttg atgcgactgc gctcattggc gggcaattgg
      601 cacaacttgt gtagcagcgc ttcaatgacc tgagcacggt agcgagtgat gtcattgtcg
      661 gtgcgggcca cgctccaggg gtacaccgct tggccgtaat aacggtcaat cggcagcggc
      721 ccggctgggg tttgccccag gttgtcgggg ttttgcgcca gcaccggttc aatcggtgcg
      781 tcgacaccga aatgggtgga aaacaggtac aaaatgagcg ggcgcgggtt gtcctgaatg
      841 gtttgcacca gcttgtcgat gacctccagg ttgacccgcc agttgttttg gtcgggctgc
      901 aacagcgcca gaagggatac cttcagggtg tagccaagtt gccaggagtg cgcccgggcc
      961 tggtcgggtt gcagtgcctg caaggtggac tccaccaggt gcgctgcaga tgcgttggga
     1021 ccggagcagc ggttcaacca aggcgcgtgc ccggccacag gagttggctg ccgagccagc
     1081 agacagccgt ccaaatccat catcggtgcc aaaaaaaagg gcgcaactgg caagcgcaac
     1141 tgccaccaca cgcacagcag aagcaccagc gggcacaggg ccagcaacag caaaaaaccg
     1201 tcgcggcgct tcattttcca agccaggatt taacccttcg ttgcaggtgg gtgggcacca
     1261 gcctggcaag tggcgaaaac gctgccgtag cccgcaaccg tgcaatggtg cgcaaggtgg
     1321 acgacttcaa aatattgcca ggcgtgttgt tgacatcgag ccaatgcaga ttgggggtca
     1381 attgctgctg tatatttttg atgtcctgtg cgttgggctc gcttgggcgc ggcagcgatt
     1441 gcaggtaact ttcccggtaa ctgaaggttt gtccattgga ccactcacgg gcaggtatct
     1501 ggctggcgct ggccgggcca atgccgctgc aaaaatgatg gtcacggata ttctcaaaaa
     1561 aatctgtcca ctggccgggg gtttgctgcc agtcacactg ccaggtccag gggcggttct
     1621 gcagtctctc ggcaaacgca ccaatatcgg gggcgatgat cggcaaaccg ctgtccaggc
     1681 tggcgctcag cgtgtagctg taggtttctg gccatacagc cggaaaccag atggcatcgg
     1741 gttgcagcca ctgcagcaac ttcatcaggt ctttgtcgtc gtaggcacca tgcaccgtca
     1801 gccgggcctt gggctgagtg cgcaagctac ggtaggcata gccaatcaaa tgaaagtcaa
     1861 tcaacaggcc ccgttgcgcg gccagagtcg ccacctcttc cagcacatcg gcccccttga
     1921 tcttgctcag cgcacccagc acagcgattt tcaggcactt ttgccggtcc agcctggccg
     1981 gcttgggttc agggtaactc ggaaccaagg tgtccaattc agaatgcggc accaccagaa
     2041 cgttggcgtt tggtgcaaag cgcgccagtc gcaaagctgt atcctgactt ggcgcaatca
     2101 cgtagcgcgc ctcgttgatc aactgcgcat gctgatcgcg ccactgctca atgccaacgc
     2161 cacctggtgc cggatggcgc tgcagacatt ggcggcattg atagagcccc tgctcaccac
     2221 aataacggtc ggtgtggtcg gtcagcgata tttgcgggca atagctgaaa aaatcgtgcg
     2281 ccgtaaagtc atggctgaca ccgagcttgc caggcaagtc aacaatgctg gcctcatgtc
     2341 ccaatagatg atggaaatga atgtgcccga cttgcagcaa gcgcaaggca ttgaccaggc
     2401 cagactgctc ttgcggcatg ccaaagtgca gcgcaaaggc ttcatgctcg ccttcgagtc
     2461 gcaaaatgac gccacccggt gcgggtgtca ggcgaaggaa tatcgcctgt tgacccagct
     2521 tttcggcaac ctcttgaata tgccgcaggg tgccaccctc acggttatga atcacattca
     2581 ggatcaccgg atgcccggct gcggtgatgc ggacaatatc cagggtcagc cgcgctgttc
     2641 gggccgggtc gcgcgccaca aaattcatca cgtcggcttc gtagcgggga tgcaggcggc
     2701 gcagcgtctg catggcctgc atctcgcgct cgctttttga gtagccaaag ctgatgccgc
     2761 cagcgtggcg aacaaaagtg tcgagcgcgt gcaggttgac ccatccggcg gcctgggcgc
     2821 gcacgcaaaa gtcgttctct tcaccatagc ctttgccaaa attttccgtg tcaaacagcc
     2881 ccacctgttg caggcattga cgccgtatgt acatgcaaaa cccgacaccg gttggcaccg
     2941 gcacggtttg tccggccagg tggctggcaa acagggcatc caggctggcg gtgttgtggc
     3001 cgctgggcaa atcattgggt ttgcagaaca ccgggtagct gcaaatcgtg gcgttgtttg
     3061 aaaacggggt cacactggct accttgggtg cgctgtaggc cgcttgcacg atgcggtcaa
     3121 gccagttgtt ggcgacctcg gtatcgctgt tgagcagcag cacatcgtgc tctgcactca
     3181 gggccatacc ccggttgacg gtgccaacaa aacccagatt ctcggggttc tcgaacagtt
     3241 gcaccctggt gtcacgaccg gccagctcac gcaaccagtc ggccagctca ggctcggggc
     3301 tgcaatcgtt gatcacaatc aaacgccagg cggcctggca gggggctgct aagaccgatt
     3361 cgaggcagcg ccgggtgtct tccaggccac gataaaccgg aaccacgatg tcaaccggat
     3421 gctgcgtaac tgccctgggc tgcgctttgt tattgggttt ttgcatggct gtgattcttt
     3481 ttgatttccc ggtcaacaag tcgagcttca ttttcaaatt gaccaaaggc cgggtcgcac
     3541 gaaacatggt ggactgctca atgccttcca gatgctgtac cagttgcgcc aggctttctg
     3601 ccagggtact gccctcaagc tgcgccgccc gaagctggct gatgagtctg accttttcgt
     3661 cgcggaaagc ttgcagtgcc agactggtgg cctgcagctt gtcctgggtt tgctgccggg
     3721 tggtctcggt ctgctgcagc gcggcttcgg tctgcgtcaa tgccagcttg cggtcatcaa
     3781 gctgccattg catcgtcgcc agtgattgct ggcgtatcac ctcctgagcg gtaaattcag
     3841 cgctggcttg caggtagtca gccgacatgg gccagcccga actgacctca agtcgggccc
     3901 cctggctgga aatctgattc aactgatggg gtgccacggg cagctcaaac caggggtcgt
     3961 caccgtgcag cagcagcagt gcattgtcgg caatcaccag cggcggggcc atcatgatct
     4021 gctgcctggg ctgggcgatc agtgtagcca gcgcgtcctg cgcggcatgc cattgccaga
     4081 ccacatcgcc gtcaggcagc gtgatgcgaa tgtggtacag cttgaaaaac cccgaccgat
     4141 cggccgggtc cagccgaatg ctggtgtaag cgacttcgct ggccggtatg tcaaacacca
     4201 gattctgtct ggggttgcca atgacgccag tggccaccaa tttgtccgac tcgtcaaacc
     4261 ggccctgcct ggccaaatag acctcggcag aaaaaagcgc ttcggcgggc tgcggggggg
     4321 gggccgttgc ggcttcggtc tggtcaggcg ctggcaacgc tgtgctgccg gacccggatg
     4381 gctgcacgac cgaaatgaac tggtaggtct gcgcatctgg cattgccagc agatgccttg
     4441 ccagcatcgg cggcaggctg tcaaaagcca ccttgaactc tgaagtcggc aagctgcgct
     4501 gcgtgacctg ggtggcttgc accgcccatc cgttttctgc aaaaaaacgc agcaatgacg
     4561 cacgggtgaa aaaccgcaag tgcgtgctgt cgagcaaacc ctccggccgg tagcgaaatt
     4621 caccctgaat cagctctgcc accaggccgc aatagcccgc gttgggcacc gaggtcagca
     4681 aacgaccgcc gggttcaagc agattcttgc attgcgccag caaattttgc ggtgccttca
     4741 ggtgctccag cacgtcggcg caaacaatgc agtcgtaacg ctggccgtca aagagctcgc
     4801 tcaggttggc gctgtcaagg tcagccacgc gggtctggcg ataccattgg ctggcaatgt
     4861 cagcctcggc ctggttcaag gtgacaccgt cggcaagcac ctcaaactgc tggctcaggt
     4921 actggccgag gccccccacg cccatgccca ggtcgagcac ggtttgcccc ggtttgatca
     4981 ggttggagat gaccgatagc gagtcatgcc ggtcgtccgc gctgacggtg cgctggtaaa
     5041 catgcggtaa agagttgtcg tttggcatga tgccaattat caatggtgcg cgcagtcgca
     5101 ctcctgtcag caaaccgtgg cccgcacccc cctcggttaa actccatttt ttccggagaa
     5161 acccaaagga accccgaaat gaccactcat gtggccgcag ctcaaggagc tctgaagatt
     5221 tttgtggcag gccatcgcgg catggtgggt tcggccattg tgcgcacctt gctggcctca
     5281 ggcgtgcccc aaaccagcat cgtcacccgc actcatgccg agcttgatct gtgcaaccag
     5341 gccgccgtgc aggccttttt tgccaccgag cggccaaccc aggtttattt ggctgccgcc
     5401 aaggtaggtg gcatttatgc caacaacacc tttccggctg acttcatcta tgacaacctg
     5461 atggtgcagg ccaatgtcat tcatgccgcc cacaccgctg acgtgcaaaa gctgctgttt
     5521 ctgggctcaa gctgcattta cccccgaatg gccgcccagc cgatgaccga gtcagccttg
     5581 ctcagcggtg ccctggaacc caccaacgag ccctatgcag tggcaaaaat tgccggcatc
     5641 aaaatgtgcg aaagctacaa ccgccagttt ggccgcgact accgcagcgt catgccgacc
     5701 aatctgtatg gccccggcga caactaccac ccccagaaca gtcacgtcat cccggcgctg
     5761 atccgccgct ttcacgaagc ccgggtggca ggtgcgccag aagtcgcaat ctggggcacc
     5821 ggcagccccc ggcgcgaatt tttgtatgtg gacgacatgg ccagtgccag cgttttcgtc
     5881 atgaacttgc cgacgctcac ctaccagacc cacacccaac ccatgcaaag ccacatcaac
     5941 gtaggtttcg gcagcgacat cacgattgac gaactgtcgc acgcggtcgg ccaggctgtg
     6001 ggctacctgg gccggatcag ctttgatgcc accaagcccg acggcgcgcc acgcaagtgg
     6061 atggacagca cccgcctgaa cagcctgggc tggcaaccca aagtggacct ggtcagcgga
     6121 ttgggcatgg cttaccgagc ctatgtccaa agcgccaaat gatgtgcaaa aacaccctcc
     6181 aggccgctcc ctataagcgc tggcagttat aaacaagata gcaaacgaag accatgacga
     6241 caaccaccag cacaacagca ttcaccccaa aggttgccct catcaccggc atcaccgggc
     6301 aggacggttc ttacctggca gagtttttgc tggaaaaagg ctaccaggtg cacggtatca
     6361 aacggcgcgc cagttcgttc aacaccgaac gcgtggacca tatttaccag gatccgcaca
     6421 tcgacaacgc acgcttcaag ctgcactacg gcgacctgtc cgacaccagc aacctgaccc
     6481 gcatcctgca agaggtgcaa cccgacgagg tttacaacct gggcgcgcaa agccatgtgg
     6541 cggtcagttt tgaaagcccc gaatacaccg ccgatgtcga tggcatgggc acgctgcgcc
     6601 tgttagaggc catccgcatc ctcggtctgg agaaaaaaac ccgcttctac caggcctcca
     6661 ccagcgagct ttttggcctg gtacaggaaa gtccgcagcg cgagacgaca ccgttttacc
     6721 ccagatcgcc gtatgccgtg gccaaactgt atgcctactg gatcaccgtc aactaccgtg
     6781 aagcctacgg catgtatgcc tgcaatggca tcttgttcaa ccacgaaagc ccgcgccggg
     6841 gcgaaacctt tgtcacccgc aagatcaccc ggggtttggc aaatatttcc cagggtctgg
     6901 agcaatgcct gtacatgggc aacatcgatg ccctgcgcga ctggggccac gcaaaagact
     6961 atgtgcgcat gcagtggctg atgttgcaac aggacgagcc gtgcgatttc gtgattgcca
     7021 ccggggtgca atacagcgtg cgccagttca tcgaaaaggc cactgcagca ctgggcatgc
     7081 agctcaggtg ggaaggcgaa ggcgttgctg aagtggccta ttggctcaac ccgaccgatg
     7141 ccgcccgcat ggcacatggc gttgacgcac cgatcatcca ggtggacccg cgctactacc
     7201 gcccgaccga ggtgcaaacc ctgctgggtg acccgaccaa ggccagggaa aagctgggct
     7261 gggtgcccga gatcacgctc gaccaactgg tgaccgaaat ggtgaccagt gacctgcaaa
     7321 aggccaggca gcatgccttg ttgaaagcca acggctacct cgtcaacgtt agcgttgaat
     7381 aagccttgtc gcttcgcatc ctccacgttg gcaagttttt cccaccacat cggggtggca
     7441 tggaatcgtt tctggccg
//
LOCUS       DAOSLL010000161         7451 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9105_length_7451_cov_3.915900, whole genome shotgun sequence.
ACCESSION   DAOSLL010000161 DAOSLL010000000
VERSION     DAOSLL010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7451
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9105_length_7451_cov_3.915900"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(110..445)
                     /locus_tag="PLB25_08780"
     CDS             complement(110..445)
                     /locus_tag="PLB25_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HOE41707.1"
                     /translation="MLYKFKSKVAGDVIMLQPNGQRVLEIIGKHSAADPSIKGILLPE
                     QMPMALTALASAIADEATARQQAVAQALAENMAPPQFEAVSLKQRALPFMDMVRDCLK
                     AQEAITWGV"
     gene            599..1642
                     /locus_tag="PLB25_08785"
     CDS             599..1642
                     /locus_tag="PLB25_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HOE41708.1"
                     /translation="MLLTGCADTAYYWQSVSGHIGLMRAARPIEDWLSSAQTPARLRE
                     RLLQAQSIRRFSVTDLHLPDNASYQSFADLRRRHAVWNVVAAPEFSLTLKTWCFPVAG
                     CVSYRGYFDQTDATALATELRAQGLEASVYGVPAYSTLGWLNWVGGDPLLSTFADYPE
                     GELARLMFHELAHQVLYVADDTAFNESFATAVERLGVAQWLATLATPQARSSYLQLAQ
                     RRTAFRLLTHATRARLAAVYMNNQPVALTRHALIAMKNEVFQDFRVAYAELKKEWGGY
                     SGFDAWVAQANNASFGAQAAYDDLVPGFEALFEREGRDWRRFYDAARQLAQLPTPERA
                     RQLQQWAKEQNLG"
     gene            1635..1982
                     /locus_tag="PLB25_08790"
     CDS             1635..1982
                     /locus_tag="PLB25_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HOE41709.1"
                     /translation="MADIHIVREHALGLSKARKIAYKWAEQVEAEFGMTCTYTEGKTG
                     DDVSFTRSGVKGSLLVTKEHFELRAQLGFLVGAFKHTIEAEIVKHLDALLAPKASDTH
                     AAKAARARAHKSV"
     gene            complement(1983..2237)
                     /locus_tag="PLB25_08795"
     CDS             complement(1983..2237)
                     /locus_tag="PLB25_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HOE41710.1"
                     /translation="MANLKTKFEAAVANSKNLSARPDNMTLLKLYALYKQGSTGDNGE
                     KRPGFTDMIGRAKWDAWNALKGTSSQDAMQQYIDLIESLS"
     gene            2398..3846
                     /locus_tag="PLB25_08800"
     CDS             2398..3846
                     /locus_tag="PLB25_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HOE41711.1"
                     /translation="MSKVDTAWLRMDCPSNLMMILGVWIIQPGVTYPAVCQRIEERLL
                     KYPRFSQRVQLDATGANWVTDVDFDIKRHVLLEVLSASVKKHPKQALQQRLGELAMQP
                     LDARYPLWDFRLVEHFDGGSALLVRIHHCIADGLALISVIQTLVDGGIDPPQRPAHVA
                     HPHGWEAAEEWMSHTLIEPLTVAAIKALEAAGDGAVHAFEAIGDPRNVLERWLEAGLS
                     KGRTGSADMARLAYQVLRDGAALVLMADDSPTRLKGTPGVVKRVAWCRPLPLDEVRAV
                     SKALHCSINDVLLACVAGAIGQYLKSFGDAVMGKEVRAMVPVNLRPMSQAYKLGNQFG
                     LAPVVLPLGLDNPIARVYEVRRRMSELKGSMQPVLAFAMLAVAGVLIKPAQDAMLNLF
                     SKKTTAVMTNVPGPREKMQFCGSTLDQALFWVPQSGSVGLGVSILSYGGGVQFGVISD
                     TTLCPDPQAIIDQFEPEFEKLSLLTMMLPWQD"
     gene            complement(3926..4699)
                     /locus_tag="PLB25_08805"
     CDS             complement(3926..4699)
                     /locus_tag="PLB25_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41712.1"
                     /translation="MARWTDFPYAGDYNFDRVSVKQKWARLHAGDLELLPQEPSLLEA
                     WAHFHNGEFQKAALMGLGLGLAGLNVANKATCIYATYLEKNEERRLALFLEVAKRAEE
                     QALREPHNINAHYWHAYALGRYSQGISVAKALAQGLDSKVKTKLETVIKHQPSHADAH
                     VVLGNFHAEIIDKVGSLIGAMAYGANRDIGLKLFAKALQLNVDSAYCMVEYARAILMI
                     DGEKMMDEATRLYQLAASVKPVDALERLEVEMAKTELED"
     gene            complement(4728..5384)
                     /locus_tag="PLB25_08810"
     CDS             complement(4728..5384)
                     /locus_tag="PLB25_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE41713.1"
                     /translation="MVKKAPRRTAERILEVTLALFNRFGEPNVSTTLISAELNISPGN
                     LYYHYPAKDELINALFDRFERNLTDLLNASEDVRNVEDAWFFMHTLFELIWQYRFLYR
                     DLNDLLSKNRRLETHFQLVLKNKTRAVKALLDGMRRGGALQIDSRETEATATSMVVVL
                     TYWLSFEYVRDPRNALEVGNAEAALLRGAQHSLNLLAPYLETSQRAHLITLADAYNKA
                     "
     gene            complement(5378..6628)
                     /locus_tag="PLB25_08815"
     CDS             complement(5378..6628)
                     /locus_tag="PLB25_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOE41714.1"
                     /translation="MTRQTAHSPKIALALAGGGPLGAIYEIGALCALHDSLKGIDFNR
                     LQHYVGVSAGGFIAAALANGITPHELFALFIEDRKHQTEVFDPAWLLRPAFAEFFARS
                     LLLPALLLSGAWQSALGRRSPLRALERLGPSLPTGIFSSDKIHTELARLFSRPGRSND
                     FRQLKSRLTLVATHLDSTTAVAFGRPGWDHVPISKAVQASAALPGLFPPVQIDGQHFV
                     DGALIKTMHASIALDEGVDLMLCLNPLVPFDASAPLTPSDRIMPWMAAEPIPRIVQGG
                     LPAVLSQTFRSLIHSRLLLGLKSYEQSYPNTDIILIEPNHRDPELYLANTFSYGQRRH
                     LAEHAYQQTRQMLRSSQTNLSAKLGYHGITLNHALLDDSQRHLCAPEKPPTRIGQALT
                     KLHNIMNNLSQVVSAVSLQSQPTW"
     gene            complement(6660..7268)
                     /locus_tag="PLB25_08820"
     CDS             complement(6660..7268)
                     /locus_tag="PLB25_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HOE41715.1"
                     /translation="MVTKLKKTTAPAKATGDKASKKAGAPLKGSVKDSAQQIWQAGLG
                     AFTKAQSEGTKAFEALVKEGISFQRKTQSAAEEKITEATQKMTSMATDISSKASGQWD
                     KLESIFEDRVAKALNKLGVPSAKDISALIARIDDLNASVQKLSPAKAAGKPSDNAAPK
                     STDQASDANGPAVKPARKPATKKAAPLKASVPEAAPDQVNPE"
BASE COUNT         1497 a         1970 c         2205 g         1779 t
ORIGIN      
        1 gcgccatttg gcagtccata gcgagcggct caatgccgcc agttgaacga tctgactgcc
       61 ggtagccacc gatcaaaaat tatctgtagg gcagcgccaa ttcaggcggc tacacccccc
      121 aggtgatggc ttcttgcgcc ttcaggcagt cgcgcaccat gtccatgaag ggcagggcgc
      181 gctgcttcag gctgaccgcc tcaaactgag gcggtgccat gttttctgcc agcgcctgtg
      241 ccactgcctg ttgtcgcgcg gtggcttcat cggcaatggc tgatgccagc gccgtcagtg
      301 ccatgggcat ttgctctggc agcaaaatac ccttgatgct gggatcggcc gcactgtgct
      361 tgccaatgat ttccagcacc cgctggccat ttggttgcaa cataattacg tccccggcaa
      421 cttttgattt gaatttgtag agcatttgaa tggttttcta ttgtgttaat tcagcaacga
      481 tgtgcttttg gctgcatgct acaccttgca cgggttgccg cttcagatgg ttagcatgtc
      541 tcggttgata agccagacaa ccgggcttgc agtacggctg ctgctgccag cgctgtcaat
      601 gctgctgact ggatgcgccg acacggccta ctattggcag tcggtcagcg gccatatcgg
      661 gttaatgcgc gcagcacggc cgattgaaga ttggctcagc agtgcgcaaa ccccggcgcg
      721 cctgcgtgag cgcttgttgc aggcgcagtc gatacggcgc ttttcggtga ctgatctgca
      781 ccttcccgac aacgccagct accagagctt tgccgatctg cggcgccgcc atgcagtgtg
      841 gaacgtggtg gcagcacccg agttctcgtt gacactgaaa acctggtgtt tcccggtcgc
      901 cgggtgcgtc agttaccgtg gttattttga ccagacagat gccacggcgc tggccaccga
      961 actgcgcgcg cagggtctgg aggcaagcgt ttatggcgtg cccgcttatt caaccctggg
     1021 ctggctgaac tgggtcggtg gtgacccgct gctcagcacc tttgccgatt accctgaggg
     1081 agaattggcg cgtttgatgt ttcacgaact ggcgcatcag gtgttgtatg tggctgatga
     1141 caccgccttc aatgagtcgt ttgcgacagc ggtagagcgc ctcggggtgg cgcagtggct
     1201 ggcaacactg gctacgccac aggcccggtc aagctacttg caattggcgc agcgacgaac
     1261 cgcgtttcgt ttgctgaccc atgccacacg tgcccgtctg gccgctgttt atatgaataa
     1321 tcagcctgta gcccttacca ggcatgcgct aatagctatg aaaaatgaag tgtttcagga
     1381 cttcagagtg gcctatgcgg agctgaaaaa agaatggggc ggctacagcg gctttgatgc
     1441 ctgggtagcg caggccaaca acgcgagctt cggcgcgcag gctgcctatg atgacctggt
     1501 gccggggttt gaggccttgt ttgagcgcga ggggcgcgat tggcggcgct tttatgatgc
     1561 tgcccgacaa ttggcacaac tgccgacacc cgagcgtgcc aggcaacttc aacaatgggc
     1621 aaaggagcaa aatcttggct gacatacata tcgttcggga acacgcattg ggtttgtcca
     1681 aagcccgcaa aatagcctac aaatgggcgg agcaggtgga agcagagttt ggcatgacct
     1741 gtacctacac cgagggcaag accggggacg atgtcagttt cacacgttcc ggagtcaaag
     1801 gcagtttgct ggtgaccaaa gagcacttcg aactgcgcgc ccaactcgga tttctggtgg
     1861 gtgccttcaa acacaccatc gaggccgaaa tagtgaaaca tctcgatgcg ctgctggccc
     1921 cgaaggcatc cgacacccac gccgcaaaag ctgcgcgagc acgagcccac aagtcagttt
     1981 gatcagctca gcgattcaat cagatcaatg tactgctgca tggcatcctg gctggatgtg
     2041 cctttgaggg cattccaggc atcccatttg gcgcggccga tcatgtcggt aaaaccgggt
     2101 cgtttctcgc cgttgtcgcc agtgctgcct tgtttgtaca aggcgtagag cttgagcagt
     2161 gtcatgttgt ccggacgcgc actcaggttt ttggaattgg caactgcggc ttcaaatttg
     2221 gttttcaggt ttgccatgaa aatagtctcc gaattggggg ttgaaccggt gtatgagggt
     2281 aatacacagc tatcttacgg tccggtttgc cgacaaaata gagaacattc ccaataatcc
     2341 aagatgaatt gattcgctat ggcgccccac ctgaaacccg tttttacagt agagcgcatg
     2401 agcaaggtcg atacggcctg gctgcgcatg gattgtccca gcaacctgat gatgatcctg
     2461 ggggtctgga tcatccagcc cggtgtgact tacccggcgg tctgccagcg cattgaagaa
     2521 cgcttgctca agtacccccg gttttcgcag cgcgtgcagt tggatgccac cggagccaac
     2581 tgggtgaccg atgttgattt cgatatcaaa cgccatgtgt tgctggaggt cttgtcggcc
     2641 tcggtcaaaa agcatccgaa gcaggccttg cagcagcgcc tgggcgaact tgcgatgcag
     2701 cccctggatg cgcgctaccc gctgtgggac tttcggttgg tggaacattt tgacggcggc
     2761 tcggccctgc tggtgcgcat tcaccactgt atcgccgatg gtctggcgct gatttccgtg
     2821 attcagacgc tggtggatgg gggcattgac ccgccgcagc gccctgcgca tgtggcccat
     2881 cctcatggct gggaagcggc cgaagagtgg atgtctcaca ccctgattga gccgctgacg
     2941 gtggctgcca tcaaggcgct agaagccgct ggcgacgggg cagtgcatgc ctttgaggcc
     3001 attggcgacc cgcgcaacgt gctggaaaga tggcttgaag ccggcttgag caaagggcgg
     3061 actggttcgg ccgacatggc gcggctggcc tatcaggtct tgcgtgacgg tgccgcgctg
     3121 gtgctgatgg ctgacgattc accaacccga ctcaagggca cgcccggcgt ggtcaagcgg
     3181 gtggcctggt gtcggccgtt gccgctcgac gaagtacgtg ccgtcagcaa ggctttgcac
     3241 tgttccatca atgacgtgtt gctggcctgt gtggccggag ccattggcca gtacctgaag
     3301 agctttggcg atgcggtgat gggcaaggaa gtccgcgcca tggtgccagt caacctgcgc
     3361 ccgatgtcgc aagcttacaa gctcggcaac cagtttgggt tagctccggt ggttttgccg
     3421 cttgggctgg acaacccgat tgccagggtg tacgaggtcc gtcgacgcat gtcagagctc
     3481 aagggcagca tgcagccggt gctggcattt gccatgctgg cagtggccgg tgtgttgatc
     3541 aaaccggccc aggacgcaat gctgaacctg ttttccaaaa agaccacagc agtcatgacc
     3601 aatgtgccgg gaccacgcga aaagatgcag ttttgtggtt caacgctgga ccaggcattg
     3661 ttctgggtgc cgcagtcggg cagcgttggc ctcggggtgt cgattttgag ctatggcggt
     3721 ggcgtgcagt ttggtgtcat cagcgacacc accctctgcc ctgacccgca ggccatcatt
     3781 gatcagttcg agccagagtt tgagaagctc agcctgctga ccatgatgct accctggcaa
     3841 gactgaattg ctgtttaaat agtagcttgt agcgcttgct gcataagggc tggaggccaa
     3901 aaagactttg catttgggaa gcagcttagt cttccagttc ggtctttgcc atttccacct
     3961 caagccgttc caaggcatca accggcttga cactggccgc cagttggtac aggcgggtgg
     4021 cctcatccat cattttttca ccatcaatca tcaggatggc gcgggcatat tcaaccatgc
     4081 aatacgccga gtccacattg agctgcaggg ctttggcaaa cagcttgagt cctatgtccc
     4141 ggttggcacc ataggccatg gcaccgatca gcgagccgac tttgtcaatg atctcggcgt
     4201 ggaaattgcc cagtaccaca tgggcatcgg catggctggg ctggtgtttg atgacggttt
     4261 cgagcttggt cttgactttg ctgtcgagcc cttgcgccaa cgcctttgcc acactgatgc
     4321 cttggctgta gcgtcccagc gcataggcat gccagtaatg cgcgttgatg ttgtgaggtt
     4381 cccgcagagc ctgttcttca gcacgttttg ccacttcaag aaacagtgcc aggcgtcttt
     4441 cctcgttctt ttccaggtag gtggcataaa tgcaggttgc cttgttggcg acgttcagac
     4501 ctgcgagccc cagcccaagt cccatcaggg ctgctttttg aaactcaccg ttgtgaaaat
     4561 gtgcccaagc ctccagcagg ctgggttcct gaggcaggag ttccaaatct cctgcgtgca
     4621 atctggccca cttctgtttg acgcttacgc gatcaaagtt gtaatcgcca gcatagggaa
     4681 aatcggtcca tctggccatt gttgtctcct gcgacccatt aaacgtgcta cgctttattg
     4741 taggcatctg ccagcgtgat caagtgcgct ctttggctgg tttccaggta aggggccagt
     4801 aaatttagcg aatgttgtgc accgcgcagc aaagccgctt cggcgttgcc cacctccagc
     4861 gcgttgcgcg ggtcgcgcac gtactcaaag ctcagccagt aagtcagcac aaccaccatg
     4921 ctggtggccg ttgcttcggt ttcacgcgaa tcaatttgca gggccccacc tcgccgcata
     4981 ccgtcgagca gggctttgac cgcgcgagtt ttgtttttta acaccagttg gaagtgcgtt
     5041 tcgaggcggc ggttcttgct caacagatca ttgaggtcac ggtacaagaa acggtattgc
     5101 cagatcagct caaacagggt gtgcatgaaa aaccaagcat cttcgacgtt gcggacatct
     5161 tcactggcgt tgagcaggtc ggtcaaattg cgctcaaagc ggtcaaacag tgcgttaatc
     5221 agctcgtctt tggctggata gtggtaatac aaattgccgg ggctgatgtt caactcggcg
     5281 gaaatcaggg tggtcgagac gttgggctcg ccaaaccggt tgaacaaggc tagcgtgact
     5341 tccagaatcc gctccgcagt gcgccttggc gcctttttta ccatgtcggc tgactctgaa
     5401 gtgacacggc actgaccact tggctcaggt tgttcatgat attgtgcagt ttggtcaatg
     5461 cttgcccaat gcgtgtgggt ggtttttccg gcgcgcacaa gtggcgttgc gaatcgtcca
     5521 acaaagcgtg attgagtgtg atgccatggt aacccagctt ggcgctcagg ttggtctggc
     5581 ttgagcgcag catctggcgg gtctgctggt aagcgtgctc ggccaagtgg cggcgttgac
     5641 catagctgaa cgtgttggcc aggtaaagct ctgggtcacg gtggttgggt tcgatcagga
     5701 taatgtcggt gttggggtag ctttgctcgt aacttttgag tccgagtaac aggcgggaat
     5761 gaatcagcga cctgaaagtc tggctgagta cggccggcag gccgccttgc acgatgcgcg
     5821 ggatcggttc tgctgccatc cagggcatga ttctgtcgct gggggtcagt ggcgctgaag
     5881 catcgaaagg aaccaacgga ttcagacaca gcatcaggtc aacaccttcg tcgagtgcga
     5941 tggacgcatg catggtcttg atcaatgcac catcaacaaa atgctgacca tcaatctgca
     6001 ctggcggaaa cagccctggt aaggcggcac tagcttgcac agcctttgaa attggcacgt
     6061 gatcccatcc aggacgacca aaagccaccg ccgtcgtgct gtccagatgg gtggccacta
     6121 gcgtcagtcg acttttaagc tgccgaaaat cattgctacg gccaggtcgc gaaaacagcc
     6181 gcgccagttc ggtatggatt ttgtcacttg aaaaaatgcc ggttggcaag cttggaccga
     6241 gtcgctccag cgctcgcagc ggcgacctgc gtcccagcgc actttgccat gcccccgaca
     6301 gcaacaacgc gggcaacaac aaggagcgtg caaaaaactc ggcaaatgct ggtctgagca
     6361 accaggccgg atcaaatact tcagtctggt gcttacgatc ttcgataaac agcgcaaaca
     6421 actcatgagg ggtgatgccg ttggccagcg cggctgctat gaagccacca gccgaaaccc
     6481 cgacatagtg ctgcaaccgg ttgaagtcga ttcctttgag ggaatcatgc agggcacaca
     6541 atgcaccgat ttcgtaaatt gcacccaatg ggccaccgcc agccagagcc aatgcaattt
     6601 tcggggagtg tgctgtctgt cgcgtcatgg gtcaaagtct aagaccgatt ctggattttt
     6661 tattcagggt ttacctgatc cggtgcggct tcaggcacag aagctttgag cggcgctgct
     6721 tttttggtgg cgggttttct ggcaggtttg accgctggac cgtttgcgtc tgatgcctgg
     6781 tcagtggatt tgggcgctgc attgtctgag ggtttgccag cagccttggc aggtgacagc
     6841 ttctgtacac tggcgttcag gtcgtcaata cgagcgatca gggcgctgat gtcttttgca
     6901 gatggcacgc caagcttgtt cagggccttg gcgacacggt cttcaaaaat gctttccagt
     6961 ttgtcccatt gaccggatgc cttggatgaa atgtcggtgg ccatgctggt catcttttgg
     7021 gtggcttcgg tgattttttc ttctgctgcc gactgtgtct tgcgctggaa actgatgcct
     7081 tccttgacca gcgcctcaaa ggccttggta ccctcgctct gggctttggt aaatgcgcca
     7141 aggcccgctt gccagatttg ctgcgcagag tccttcactg agcctttcag gggtgcgcct
     7201 gcttttttgc tggccttgtc gccagtggct ttggcgggag cagttgtttt tttcagtttt
     7261 gtaaccatgg tgttctccgg ttgaattcaa aaaatgagtg ggtaaattgc agattggctg
     7321 atccgaatgt ggcggtactg tagagcgcaa gccgtgcttg gtgtaggtgc atctttctaa
     7381 tctgaggggg caacttattg gttcttcgtc gttttgtgtg gaagttgcat tgaaaaataa
     7441 aatgtagtag t
//
LOCUS       DAOSLL010000162         7415 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9159_length_7415_cov_3.668478, whole genome shotgun sequence.
ACCESSION   DAOSLL010000162 DAOSLL010000000
VERSION     DAOSLL010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7415
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9159_length_7415_cov_3.668478"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="PLB25_08825"
     CDS             <1..854
                     /locus_tag="PLB25_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE41716.1"
                     /translation="MVIATDITARRLAEEQSEVQAERAQASSRLITMGEMASSVAHEL
                     NQPLTAINNYCNGMVSRIKGKQIDEQELLGALEKTARQAHRAGQIIQRIRSFVKRSEP
                     NRTNASVANMVSEAVELAEIELRRRNVKLTQYVAPGMPSIVVDPILIEQVLLNLLKNA
                     AESVDAAQRPTALRLIKLRVAPGQVDGKPVIEFKVIDSGDGIAPEVMARLYEAFYSTK
                     TEGMGIGLSLCRSIVESHMGRMKAENLYNGQVVSGCCFTFWIPMTDLKPAASHQRSDQ
                     QGFQVLS"
     gene            851..1477
                     /locus_tag="PLB25_08830"
     CDS             851..1477
                     /locus_tag="PLB25_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE41717.1"
                     /translation="MSLIPKKGTVYVVDDDEAVRDSLQWLLEGKDYRVRCFDSAESFL
                     SRFDAREVACLIVDIRMAGMNGLELQNRLIESHSPLPLAFITGHGDVPMAVDTMKKGA
                     MDFIQKPFDEEQLVPLVERMLDQARGSFAEYQSALTRDALMARLTLRESQVLERIVAG
                     RLNKQIADDLGISIKTVEAHRANIMEKLGANTVADLLKIALGPNAAKA"
     gene            1570..2436
                     /gene="folD"
                     /locus_tag="PLB25_08835"
     CDS             1570..2436
                     /gene="folD"
                     /locus_tag="PLB25_08835"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HOE41718.1"
                     /translation="MTNPSALAQIIDGVALAHQLRADVARRVQALKLRGITPGLAVIL
                     VGDNPASQVYVRNKVKACEDVGLHSVLERYNADMQQAALLARIEALNQDAAIHGILVQ
                     LPVPAQIDATKVLEAISPAKDVDGFHIASAGALLTGMPGFLPCTPYGCMKMLESIHFD
                     LRGKHAVVIGRSNIVGKPMALLLLQKHATVTICHSATQNLKALTLQADLVVAAVGKPN
                     VLTADMVKPGAVVLDVGMNRNDAGKLCGDVDFEGVRQVAGFISPVPGGVGPMTITMLL
                     VNTLEAAERSAN"
     gene            2622..4676
                     /locus_tag="PLB25_08840"
     CDS             2622..4676
                     /locus_tag="PLB25_08840"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HOE41719.1"
                     /translation="MTNPLLSFDDLPQFDQISPEHVEPAIDLLLKRANAALEQVTAPD
                     FAPSWNAIAMLLDVATEQLSRAWGVVSHLNSVADAPELRAAYNAALPRITEFYTRLGA
                     DERLYAKYKTIAPTSLTAEQRQAHKNALRNFVLGGADLQGPQRQRFAEIQERQAAVSQ
                     KFSENTLDATDAFAYYAQSDELDGVPPDVVHTALQAAQAEGKDGYRLTLKMPCYLPVM
                     QFASSSALREKLYHAYATRASDQAAPDARQFDNTDLIRELLALRLEEARLLGLPNFAT
                     LSLVPKMAESPGQVIRFLRDLANRARPFAEKDLADMRAFGLEHLNLPNPQPWDWPYVG
                     EKLKEARYAFNEQEVKQYFPLPTVLAGLFKIVETLFEVSISEDHAALWHKSVHFYRIE
                     RAAAQTGDQHTVLLGQFYLDPAARNGKRGGAWMDDVRARWLRPDTQQLQTPVAHLVCN
                     FASGAGGKPALLTHDDVTTLFHEFGHGLQHLLTQVNERDVSGISGVEWDAVELPSQFM
                     ENFCWEWDVLKNMSAHVDTGQPLPRALFDKMLAAKNYQSGLQTLRQIEFSLFDMLLHT
                     DHDPAQDILQLQLQVRQEVAVLQPPAWARSANTFSHIFAGGYAAGYYSYKWAEVLSAD
                     AYAAFEETTGTDGLPNLQTGRNFRQQVLETGGSRPAMASFRAFRGREPTLDALLRHQG
                     MA"
     gene            4703..5371
                     /locus_tag="PLB25_08845"
     CDS             4703..5371
                     /locus_tag="PLB25_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HOE41720.1"
                     /translation="MQRSPLILKCGGVGAARWLSGLILLGTLAALTPAQAQPVYRIVG
                     PDGKITFSDKPPGAAAKGTGLETSTPASPGGSALPYELRQVAEKYPVVLYTSRDCAPC
                     DSGRSLLRGRGVPFAEKTVTSSDDAESLQRLSGSTSLPFLTIGGQQIKGFSDAEWVQY
                     LTAAGYPEKSKLPTGYRNPQATPLVAVKQPDAAAPGNSVPTAEPIPAGPPRVNSSNPA
                     GIQF"
     gene            complement(5446..6690)
                     /locus_tag="PLB25_08850"
     CDS             complement(5446..6690)
                     /locus_tag="PLB25_08850"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HOE41721.1"
                     /translation="MKRVDDFRLMFGKKELVPIIVGGMGVDISTAELALEAARLGGIG
                     HISDAMVNDVSDRRFDTSFVKDKTRLYKHNILNADKSGIKFDLDILAEATRRHVGATM
                     EAKKGDGLIFVNCMEKLTMNGPRETLQVRMASALDAGIDGITLSAGLHLGSFGLIAGH
                     SRFRDAKLGIIVSSVRALQLFLRKNAKLNRLPDFVVVEGPLAGGHLGFGLDWAQYDLA
                     TIIAEVIKYVNDEQLGIPVIAAGGIFTGSDAVSFLQTGSAAVQVATRFTVSNECGLPA
                     NVKQDYFLASEADIEVNTISPTGYPMRMLKGSPAIGDGIRPNCESYGYLLDGAGNCAY
                     ITAYNRELALHPDGKNIKVLDKTCLCTQMRNFKLWTCGHYTYRLKDTTLRNADGSYQT
                     LSAEHIFKDYQFSVDQQVALPA"
     gene            complement(6928..7254)
                     /locus_tag="PLB25_08855"
     CDS             complement(6928..7254)
                     /locus_tag="PLB25_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41722.1"
                     /translation="MNPKRLLYSVKKIQQVDHPRMVINTNKTKLRTIAQLQELLNATL
                     EISFSNIGENGDSERNEHISRVLKRFDYPQCKKHEWRDRVFLQDTSGYSRARVKRLLA
                     RPTGFV"
BASE COUNT         1612 a         2184 c         2084 g         1535 t
ORIGIN      
        1 aaatggtgat tgccaccgac atcacggcac gccgcctggc cgaagagcag tccgaggtgc
       61 aggccgaacg ggcccaggca tcaagccgat tgatcaccat gggtgagatg gcttccagcg
      121 tggcacatga actcaaccag ccgctgactg ccatcaacaa ctattgcaac ggcatggtgt
      181 cgcgcatcaa gggcaagcaa atcgacgagc aggaattgct cggggcgctg gaaaaaactg
      241 cccgccaggc acaccgggcc gggcagatca tccagcgtat ccgctcgttt gtgaagcgca
      301 gcgagcccaa ccgcaccaat gccagcgtcg ccaacatggt ctccgaagcg gtggaattgg
      361 ctgaaatcga actgcgtcgg cgcaatgtga aactgacgca gtacgtggca ccgggcatgc
      421 caagcattgt ggtggacccg atcctgatcg aacaggtact gctcaacctg ctgaaaaacg
      481 cggccgaatc ggttgacgcg gcgcagcgcc ccaccgcact gcgtctgatc aagctgcgcg
      541 tggcaccggg tcaggtcgat ggcaagccgg tgatcgagtt caaggtgatc gactctggcg
      601 acggcattgc acccgaagtc atggcccgcc tttatgaggc gttttactca accaagaccg
      661 agggcatggg cattggcctg tccttgtgcc gctccatcgt ggagtcgcac atgggcagga
      721 tgaaagccga gaacctctac aatggacagg tcgtaagcgg ttgctgcttt actttctgga
      781 tacccatgac agacctgaag cccgcagcat cgcatcagcg ttctgatcaa caaggatttc
      841 aggttttatc atgagtttga ttcccaaaaa gggcacggtc tatgtcgttg acgacgacga
      901 ggcagtgcgc gattccttgc aatggctgct cgaaggcaaa gactaccggg tgcgctgctt
      961 tgactctgcc gagtccttct tgagccgctt tgacgcgcgc gaggtagcct gcctgattgt
     1021 cgatatccgc atggctggca tgaacgggtt ggaactacaa aatcgcctga tcgagagcca
     1081 ctcaccgctg ccgctggcct tcattactgg ccacggcgac gtgccaatgg cagtcgatac
     1141 gatgaaaaaa ggtgccatgg actttatcca gaagcctttt gacgaagaac aactggtgcc
     1201 actggttgag cgcatgctgg accaggcgcg cggctcgttt gccgaatacc aaagcgcact
     1261 cacccgcgac gcactgatgg ccagactgac cctgcgcgag tcgcaggtgc ttgagcgcat
     1321 cgtcgcgggt cggctcaaca agcagattgc cgacgacctg ggcatcagca tcaagacggt
     1381 agaagcacac cgtgccaaca tcatggaaaa gctcggcgcc aacaccgtgg ccgatttgct
     1441 gaaaattgca ctcgggccaa atgccgcaaa ggcctgattt cagccctctt caattgctat
     1501 ctaaaatata gctagctgcg cccgtcccat aagggctaac gggctttttt atatataact
     1561 tttttgacca tgacgaaccc atcagccctg gcccagatca tcgacggtgt ggcgctggca
     1621 caccaattgc gcgctgacgt ggcgcgccgg gtgcaagccc tcaagctgcg tggcatcaca
     1681 cccggcctgg cggtgatcct ggttggcgac aatccggcca gccaggtcta tgtgcgcaac
     1741 aaggtcaagg cgtgtgaaga tgtcggactg cattcggtgc tggaaaggta caacgccgac
     1801 atgcagcaag ctgccctgct ggcgcgcatt gaggcgctca atcaagatgc cgccattcac
     1861 ggtattttgg tgcaattgcc tgttcccgcc cagattgatg ccaccaaggt gttggaggcg
     1921 atttcaccgg ccaaggatgt cgatggcttt cacattgcca gtgccggtgc cttgctgaca
     1981 ggcatgcccg gctttttgcc gtgcaccccc tatggctgca tgaaaatgct tgagtcgatc
     2041 cactttgacc tgcgcggcaa acatgcggtg gtgattgggc gcagcaatat tgtgggcaaa
     2101 ccgatggcct tgctgctgct gcaaaaacac gcaaccgtca ccatttgcca cagcgctacc
     2161 caaaacttga aagctctgac gctgcaggct gatttggtgg tggcggcggt cggcaagccc
     2221 aatgtgctca ccgccgacat ggtcaagccc ggtgcggtgg tgctcgatgt cggcatgaac
     2281 cgcaatgacg ctggcaaact gtgcggcgat gttgattttg aaggcgtgcg ccaagtggca
     2341 ggcttcatca gcccggtgcc tggcggggtt ggcccaatga ccatcaccat gttgctggtc
     2401 aacacgctgg aagccgccga gcgcagcgcc aactgagctt gccgcgcagc catctgcagc
     2461 ggcaacataa atcagcgcgc tgatttcggg aactcaatca gcgcggccat catccaagtt
     2521 acccatgcat tcatggctgt ccgaacggtg aaaaccggtc ggcccgttca ttcgattttt
     2581 tgaacctcaa cgcgccccat gctcgttctc tcaggtaaat tatgacgaac cccctgcttt
     2641 cttttgacga cctgccgcag tttgaccaaa tcagtcccga gcatgttgaa cctgctatcg
     2701 atctgttgct caaacgcgcc aacgcggcgc tggagcaagt cacggcacct gactttgcgc
     2761 caagctggaa tgccattgcc atgttgctgg acgtggccac cgaacagcta tcgcgcgcct
     2821 ggggtgtcgt cagtcacttg aacagcgtgg ccgatgcgcc cgaactgcgc gccgcctaca
     2881 acgcagcact gccccgaatc accgagtttt acacgcgcct gggtgccgat gaacggctgt
     2941 atgccaaata caaaaccatt gcgccgacca gcctgacagc cgagcagcgc caggcgcaca
     3001 agaatgcctt gcgcaacttt gttctcggtg gtgccgactt gcaggggcca caacgccagc
     3061 gctttgccga aattcaggag cgccaggctg cggtcagcca gaaattcagt gagaacacac
     3121 tggatgccac cgatgctttt gcctactacg cccaatccga cgagctcgat ggtgttccgc
     3181 ctgatgtcgt gcacaccgcc ctgcaggccg cacaggcaga gggcaaagac ggctaccggc
     3241 tgaccctcaa gatgccttgt tacctgccgg tgatgcagtt tgccagcagc agtgcgctgc
     3301 gtgaaaagct ctaccacgcc tatgccacgc gagcctccga ccaggcagcc cctgacgcgc
     3361 gccagtttga caacactgat ctgatcaggg aactgctggc attgcggctg gaagaagcgc
     3421 ggctgctggg tttgcccaac tttgccacac tgtcgttagt gccgaagatg gccgagtcgc
     3481 ccgggcaagt catccgcttt ttgcgcgatc tggccaaccg cgcgcgaccg tttgcagaaa
     3541 aagacctggc tgacatgcgg gcttttggcc ttgagcacct gaacctgccc aatccgcagc
     3601 catgggactg gccttatgtg ggtgaaaagc tcaaggaagc gcgctacgcc ttcaacgaac
     3661 aagaagtcaa acagtatttc cccctgccga ccgtgctggc tggcctgttc aagatcgtcg
     3721 aaacactgtt tgaagtcagc atcagcgaag accatgcggc gctctggcat aaatcggtgc
     3781 atttttaccg cattgagcgt gccgctgccc agaccggtga tcagcacacg gtgctgcttg
     3841 gccagtttta tctggaccca gccgcccgca acggcaaacg cggtggagcc tggatggacg
     3901 atgtgcgcgc gcgctggcta cgccccgaca cgcagcagtt gcaaacgcca gtcgcccatc
     3961 tggtgtgcaa ttttgcctct ggagcaggtg gcaagccagc actgctcaca cacgatgatg
     4021 tcaccacctt gttccatgaa tttggtcacg gcctgcagca cctgctgaca caggtcaatg
     4081 aacgggacgt gtctggcatc agcggtgtcg agtgggacgc ggtggaactg cccagccagt
     4141 tcatggagaa cttttgctgg gagtgggacg tgctgaagaa catgagcgcc cacgttgata
     4201 ccggtcagcc gttgccgcgc gcgctgtttg acaagatgtt ggcggccaaa aactaccaaa
     4261 gcggcctgca gacgctgcgc cagatcgagt tttcgctgtt tgacatgctg ttgcacactg
     4321 accatgaccc ggcgcaagac attctgcaac tgcagttgca agtgcgccag gaagttgccg
     4381 tgctgcagcc tcccgcctgg gcgcgcagcg ccaacacctt cagccatatc tttgctggcg
     4441 gttacgccgc cggctattac agctacaaat gggctgaggt gctgagtgca gatgcctacg
     4501 cagccttcga ggaaaccacg ggcacagacg gcttgcccaa tctgcaaaca ggccgaaact
     4561 tccgccagca ggtccttgaa acgggcggca gtcgcccggc catggcatca ttcagggcgt
     4621 ttcgcgggcg cgaaccaaca ttggacgcac tgctgcgcca ccagggcatg gcatagtagg
     4681 aaacaagaca aggaggttga agatgcaacg ttcaccattg attttgaagt gcggcggggt
     4741 gggtgccgcg cgctggttgt ctggcctgat tctgctaggc acgttggccg cactgacccc
     4801 ggcacaggcg cagccggttt accgcatcgt cggaccggat ggcaagatca ccttctcaga
     4861 caaaccacct ggggctgcag ccaaaggcac cggccttgag acaagcactc cggctagccc
     4921 aggcggctct gccctgcctt atgagttgag acaggtggct gaaaaatacc cggttgtcct
     4981 gtacacatcg cgcgactgtg cgccatgcga tagtggccgc agcttgctgc gcgggcgcgg
     5041 tgtgccgttt gccgaaaaaa ccgtgacatc ttccgacgat gcagagtctt tgcagcgcct
     5101 cagtggcagt acctcgctgc cgtttctgac catcggcggc cagcagatca agggcttttc
     5161 cgatgccgaa tgggtgcagt acctgacggc agccggttac cccgaaaaga gcaagttgcc
     5221 aactggctac cgcaacccgc aagccacacc gctggttgcc gtcaagcagc ctgatgcagc
     5281 ggcaccaggc aacagcgtgc ccaccgcaga accaataccg gcgggaccgc cccgggtcaa
     5341 ttcatctaac ccggcgggca tccagtttta gtctgagctg ggcagccata aaaaagcccc
     5401 tggtcaaaca acctggggct tttttgacga actgccatgc aaacttcaag cgggcaacgc
     5461 cacctgctga tctacactga actggtagtc cttgaagatg tgctcggcac tcaaggtctg
     5521 gtaactgccg tctgcgttgc gcagcgtggt gtccttgagg cggtaggtgt aatgcccgca
     5581 ggtccagagc ttgaagttgc gcatctgggt gcacaggcag gtcttgtcca agaccttgat
     5641 attcttgcca tcgggatgca gcgccagttc gcggttatag gccgtgatgt aggcacaatt
     5701 tccggcgcca tccagcaaat aaccgtacga ctcgcaattg ggacgaatac cgtcaccaat
     5761 ggcaggcgaa cccttcaaca tgcgcatcgg gtagccagtg ggcgagatgg tattgacttc
     5821 gatgtcggct tcactggcca aaaaatagtc ctgcttgacg ttggctggca ggccgcattc
     5881 gttggacaca gtgaagcggg tagcgacctg tacggcggcc gagccggtct gcaaaaacga
     5941 caccgcgtcg ctcccagtaa aaatgccgcc tgcggcaatc actggaatcc ccaactgctc
     6001 gtcgttcaca tacttgatca cctcggcgat gatggtggcc aggtcgtact gcgcccaatc
     6061 cagcccaaaa cccagatgac caccggccaa cgggccttct accacgacaa aatccggcag
     6121 gcggtttagc ttggcgttct tgcgcaaaaa cagttgcaac gcccgcacgc ttgagacaat
     6181 gatgccaagt ttggcatcgc gaaaccggga atgcccggca atcaggccaa acgagcccag
     6241 atgcaaacca gcgctcaagg taataccatc aatgccggca tccagggcgg atgccatgcg
     6301 cacctgcagg gtttcacgcg ggccgttcat ggtcagcttt tccatgcaat tgacaaaaat
     6361 caggccatcg ccctttttgg cttccatggt ggcaccaaca tgacgccgtg tcgcttcagc
     6421 caaaatgtcc aggtcgaatt tgatcccgga tttgtcggcg ttgagtatgt tgtgtttgta
     6481 cagtctggtc ttgtctttga cgaaactggt atcaaaccgc cggtccgaca catcgttgac
     6541 catggcatcc gaaatatgac caatgccacc tagccgtgcc gcctcaaggg ccagttcggc
     6601 tgttgaaata tcgacaccca tgccccctac gatgatcggc actagctctt ttttgccaaa
     6661 cattaggcga aaatcgtcta cacgcttcat gcaaatcctt gatccattcc ttgaaccgta
     6721 cagctccttt ggacaggcca aaacccgcgc caaacccagg cgcaacttgg gtaaagaagc
     6781 aaatattgac aagacaaaat gatcttgtcg ggctgcgatt ctacctgcgc cccccctgtg
     6841 cctcatgaca aggtcaatac ccatgacgcg cttgtactgt tgattccgtg tgactactct
     6901 gcccgtcgtt aggcaagccc actgttctca gacgaagccg gtcgggcgcg ccaacagtcg
     6961 tttgacccgg gcgcggctgt agccgctggt gtcctgcaag aagaccctat cacgccactc
     7021 atgtttcttg cactgggggt agtcaaagcg cttgagcacc cggctgatgt gctcgtttct
     7081 ttcgctgtca ccgttctcgc caatattact gaagctgatc tcgagcgttg cgttgagaag
     7141 ttcttgcagt tgcgcaattg tgcgcagctt tgttttgttc gtgttgatca ccatccttgg
     7201 atgatcaacc tgctggattt tttttaccga gtataagagc cgctttgggt tcattcttgg
     7261 gtggaaatgc ccacgctgcg caggctcaca ccttgtcgga caagaccaat cgatgatcgt
     7321 tttctagtcc tttcgaggtc agctataatg aggcctctaa tcctcgatag ctcagttggt
     7381 agagcgccgg actgttaatc cgtaggtccc tggtt
//
LOCUS       DAOSLL010000163         7372 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9232_length_7372_cov_3.888889, whole genome shotgun sequence.
ACCESSION   DAOSLL010000163 DAOSLL010000000
VERSION     DAOSLL010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9232_length_7372_cov_3.888889"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..919
                     /locus_tag="PLB25_08860"
     CDS             137..919
                     /locus_tag="PLB25_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41723.1"
                     /translation="METAPTVPSGSSGTSSGPSFGNPSPTAGSSTSNQANAAADAAAY
                     KEIAYKNRDKKGPALVVIPGEIKSNNATFTQKFTPNNIADFAELELSQANFQVLERAN
                     LGPLLNEISLAYNLGDPAKARKTMQAGKLKTTKWIVKFDILKTEQIASNTQGFDGRAV
                     GNLIGVLGHSRASAAGSTVVGSVQTSDSTGVWLIGMRYKIMNASTTAQVATGYKELKM
                     EVGATSTSVMGISSSAKGGVGLDTMVQRLVQTAVWDIDAKNK"
     gene            938..1519
                     /locus_tag="PLB25_08865"
     CDS             938..1519
                     /locus_tag="PLB25_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HOE41724.1"
                     /translation="MNSSVWKLFLSVALALGVAGCSKNEAPPVAPPPAAITSSSYDMV
                     ASGAKGFTVGALMSAQPVYVLFDPQCPHCGHLWNASLPLHAKVKFVWVPIAFNSGKSL
                     AQAVALLTASDPLAAMSAHEQSLLAGQGGMAASSSMAGEQQQAIKANTQLLTALGVDS
                     VPFILAKNRRTGAVVSNSGALNTAALAQLLGVE"
     gene            complement(1558..2463)
                     /locus_tag="PLB25_08870"
     CDS             complement(1558..2463)
                     /locus_tag="PLB25_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOE41725.1"
                     /translation="MMSLNLPVSPDKNDPLPLVLYHGRSCPDGFAAALAAWLYFDGKA
                     QFLGLDHGDVNSLDDLPALAGRAVYVLDFSFAEPLLRAVEERAAKLVLLDHHLSAATR
                     LTGFRCRNGVVHFDMNKSGAHLAWEFFWPETPLPDLVRFVEDRDIWVWQYPQSAGFLA
                     ALDMEPFDFVRWQAIVGFDAAQLHAYIARGSAMNDKFNRLATQIAENACAIRFNGIDG
                     LMVNAPGVFHSQVGDLLSQKSGTFALLWTVDKNAAVKCGLRSQTPFNCIALAESMGGG
                     GHAQACGFRMPHQRLAELLGGNLNA"
     gene            complement(2572..3504)
                     /gene="ylqF"
                     /locus_tag="PLB25_08875"
     CDS             complement(2572..3504)
                     /gene="ylqF"
                     /locus_tag="PLB25_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HOE41726.1"
                     /translation="MAIQWFPGHMHLTQKAISERIKHIDVIVELLDARLPGSSANPML
                     ATLTQGKPTLKVLNKQDLADPECTALWLAHYNALPGTRAIALDASVTAPARALIAACH
                     ELSPGRRGMAKPMRVLICGIPNVGKSTLINTLTGRRATKTGDEAGVTKLEQRIALAPD
                     FYLYDTPGILWPRIIVAKSGYNLAASGAVGRNAFDEQEVALALLDYLANHYPVLLQTR
                     FKIDAVAALTDEQVLEAIGRKRGALKGGQQVNLQKAAEMAIHDFRAGLVGRITLETPA
                     EFAQWLAAGQTLDAQRQVKKDAIELDRKIRFKQR"
     gene            complement(3507..5450)
                     /locus_tag="PLB25_08880"
     CDS             complement(3507..5450)
                     /locus_tag="PLB25_08880"
                     /inference="COORDINATES: protein motif:HMM:TIGR03062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HOE41727.1"
                     /translation="MRPEVGQIVTFVRIARIEALFFLHYPKLLLGAAVVVLIPALYVL
                     IYLTSVWDPAAKTGALPVAIVNLDHGFEYRQHVFNVGQDLAGRLKSKPAFGYVDYTDE
                     QQARRLVRQGELAFALIVPLDFSSNAVPGAEAGAGKLVVFASEGNSYPSAGLARRFAE
                     DLGREVNQSLNEQRWALVLADAAGSQRSMAQLHQGVQQLRLGARELASGVQQSALGAQ
                     GLANGSGKLDQGIGQLTSGVRELGGGLRSMFGQRPKNSELRRLDEGAQALVDGHEELK
                     GGLLQLQQGTQRLQTGVNGFREEASSSTFIATRVKEGLNQLGEGVSTLETGLKNASAA
                     QQKLADGSTQLNTGVATLTTGLRSLNNGLRSMVTQLPEDSKLSELARGSASLSSGASA
                     LKTGTQKLSLAAQHLAGGLELLEDALPASVKKMDGNAQGLATSVQPVMEIDARVLNNG
                     SGFAPNIVPGALWLGASLAVFLIQARLLPLFAAQMSPLARLLGKMLIPMHIALLQALL
                     VWVTVVWILEVQAVDALALALTVGVASLTFLLIIFALIRVFGDGGKALALVLLAVQLS
                     SSGGVLPVELSGGLFARISPYLPITWVVKAIKASLFGAFEGDWQHPLQLVVLAGVVAA
                     VMACFVGRWRFVKVSALRPSLDL"
     gene            complement(5440..5850)
                     /gene="arfB"
                     /locus_tag="PLB25_08885"
     CDS             complement(5440..5850)
                     /gene="arfB"
                     /locus_tag="PLB25_08885"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008117295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HOE41728.1"
                     /translation="MTELLQVRDDELELKPIRAQGAGGQNVNKVSSAIHLRFDVLSSS
                     LPGDVKTRLLALRDSRISQDGVVVIKAQQHRTQEMNRFDAVVRLQELVNSVARAPKTR
                     RPTKPTRASRQRLRAQKTERSGLKAQRAPVRHET"
     gene            complement(5872..7257)
                     /gene="yegQ"
                     /locus_tag="PLB25_08890"
     CDS             complement(5872..7257)
                     /gene="yegQ"
                     /locus_tag="PLB25_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HOE41729.1"
                     /translation="MKPPELLAPAGSLKMLETALAFGANAVYAGQPRYSLRVRNNDFG
                     DLQVLQQGIDTTHARGAKFFLVSNIFPHGNKIKHYVSNMAPVIALKPDAMIMSDPGLI
                     MMVRETWPDMEIHLSVQANTVNAAAVRFWKTVGVSRVILSRELSLDQVEQIRQDCPDT
                     ELEVFVHGALCIAYSGRCLLSGYFNHRDANQGSCTNSCRWDYKTQKAVVDASGDILAP
                     QTAFQRNQAELQRVADCQTEGLERVSEANEVYLLEEGQRPGSYMPIEEDEHGTYVMNS
                     RDLRAIEHVQRLVAIGVDSLKIEGRTKSPYYVARTVQSYRRAIDDALAGRELDPALLG
                     QLEGLANRGYTSGFFQRHTPEATQNYLRGYSESGRSLYVGDVLSFDAVRGLAQVQVKN
                     RFAVGDWLEIIAPAGNVDVQLSHIENQDAEPMSVAPGSGHTVWLPLPQSSVGAFVARY
                     VSAPESQAAAA"
BASE COUNT         1503 a         2263 c         2162 g         1444 t
ORIGIN      
        1 caaattaggt tgtatgcttg caccttcgaa tgcagagatc ttgccttcta ctttgaaagt
       61 gaccccgacc atgtacaccc actcaatcaa gaccgccgca gtctcggtcg caatggcctg
      121 cctgatgact gcctgcatgg agactgcgcc aaccgttcct tcaggcagct cgggcacatc
      181 ctctggccct tcgttcggca atccatcacc gactgcgggc agttccacct cgaaccaggc
      241 caatgcggcg gctgatgctg ccgcctacaa agagattgcg tacaagaacc gggacaaaaa
      301 gggtccggcc ctggtggtga ttccgggcga aatcaagagc aacaatgcca cgttcaccca
      361 aaaattcacc cccaacaaca ttgctgattt tgccgagctg gaattgtcgc aagccaattt
      421 ccaggtgctg gagcgtgcca acttgggccc gctgctcaat gaaatttcgc tcgcctataa
      481 cctgggcgac ccggccaagg cacgcaagac gatgcaggcc ggcaaactca agacgaccaa
      541 gtggattgtc aagtttgaca ttctgaaaac cgagcagatc gccagcaata cccaggggtt
      601 tgacggtcgt gcggttggca atctgatcgg ggtcctgggc catagccggg cctctgcggc
      661 tggcagcacc gttgtaggtt cagtccagac cagtgacagt acaggcgtct ggctgattgg
      721 catgcgctac aagatcatga acgccagcac caccgcacag gtggcaaccg ggtacaagga
      781 actcaagatg gaagttggtg ccaccagcac ctccgtgatg ggcatttcgt ccagcgccaa
      841 aggtggtgtc ggcctcgaca ccatggtgca gcgcctggtg cagacggcgg tctgggacat
      901 tgatgccaaa aacaaataac acctgatcgg ttgccaagtg aactcatctg tctggaaact
      961 attcctgagc gttgccttgg ccttgggggt tgcaggctgc tcaaaaaacg aagcaccccc
     1021 ggttgcgccg ccgcctgccg ccatcaccag cagcagctac gacatggtcg ccagcggtgc
     1081 caagggcttt accgtgggcg ccctgatgag cgcccagccg gtttatgtgt tgtttgatcc
     1141 ccagtgcccg cactgtggcc acctctggaa tgcgtcgctg ccactgcacg ccaaggtgaa
     1201 gtttgtctgg gtgccgattg ccttcaactc gggcaagagt ctggcccagg cggtggcctt
     1261 gctgaccgcc agcgacccgc tggcagccat gtcggcgcac gaacagtcct tgctggccgg
     1321 tcagggtggc atggccgcgt ccagcagcat ggcgggtgaa cagcagcaag ccatcaaggc
     1381 caacacccag cttttaactg ccctcggtgt tgactcggtg ccctttatcc tggccaaaaa
     1441 ccggcgcacc ggtgcggtgg tcagcaacag cggtgcgctg aacaccgcag cactggcgca
     1501 gttgctgggt gtggagtagt tgacgcgcag cacgggtggg ccgggcagcg gtgggtttca
     1561 ggcgttcagg ttgccgccta acagctcggc caggcgctgg tggggcattc tgaaaccgca
     1621 agcctgcgcg tgaccgccac cgcccatgct ctcggccagc gcgatgcaat tgaaaggcgt
     1681 ttgtgaacgc agaccacact tgacggctgc gtttttatcg actgtccaga gcaaggcaaa
     1741 ggtgcccgat ttttggctca gcaagtcgcc gacctggctg tgaaaaacgc ccggcgcgtt
     1801 gaccatcagg ccgtcaatgc cgttgaaacg gatggcacag gcgttctcgg cgatctgtgt
     1861 tgcgagccgg ttgaacttgt cattcatggc gctgccacgc gcaatgtagg cgtgcaactg
     1921 tgctgcatca aacccgacaa tcgcctgcca gcggacaaag tcgaaaggct ccatgtccag
     1981 cgcggccagg aaacccgcgc tctgcgggta ctgccagacc cagatgtcgc ggtcttccac
     2041 aaatctcacc aggtctggca gtggcgtttc tggccagaag aactcccagg ccaggtgtgc
     2101 acctgacttg ttcatgtcaa agtgcaccac gccgttgcgg cagcgaaagc cggtcagcct
     2161 ggtcgccgcg ctgaggtggt ggtccagcag caccagtttg gcggcccgct cttcaacagc
     2221 gcgcagcagg ggttcggcaa atgaaaaatc cagcacgtaa acggcccgcc cggccagtgc
     2281 tggcaagtcg tccagactgt tgacatcgcc atggtcgagc cccagaaatt gcgctttgcc
     2341 gtcgaaatac agccaggccg ccagcgccgc cgcaaagccg tccgggcaac tgcggccgtg
     2401 gtagagcacc agcgggagcg ggtcattttt atctggagaa accggcaaat tcaaactcat
     2461 catgtactca atcaaaaaca gttgccgcta aaaatgctat atagttaata gcttccaacg
     2521 caacagacat aagggctaga agccaattgt tatcaaaaaa accaggcaga ttcaacgctg
     2581 cttgaacctg atcttgcggt ccagttcgat cgcatccttc ttgacctgcc gctgggcatc
     2641 cagggtttgc ccggctgcca gccactgggc gaattctgcc ggggtttcca gggtgatgcg
     2701 gcccaccagg ccggcgcgaa agtcatgaat cgccatctcg gcggcttttt gcaagttcac
     2761 ctgttgacca cccttcagag cgccacgctt gcgaccgatc gcctccagca cctgctcatc
     2821 ggttagcgca gccacagcgt ctatcttgaa acgcgtttgc agcagcaccg ggtaatggtt
     2881 tgccagatag tccagcaggg ccagtgccac ttcctgctcg tcaaaggcgt tgcgacccac
     2941 cgcaccgctg gcagccaggt tgtagccact tttggccaca atgatgcgcg gccaaagaat
     3001 gcctggcgtg tcatacaggt aaaagtccgg ggctagcgca atgcgctgct ccagcttggt
     3061 gacacccgct tcatcgccgg ttttggtggc ccgcctgcct gtcaaggtgt tgatcagggt
     3121 cgatttgccg acattgggaa taccgcaaat cagcacccgc atcggcttgg ccatgccacg
     3181 gcggccgggg ctgagttcat gacaggccgc tatcagggcc cgggccggtg cggtcacgct
     3241 ggcatcgagt gcgatggcac gggtgccggg caaggcgttg tagtgcgcca gccagagcgc
     3301 ggtgcactcg gggtcggcca ggtcttgctt gttgagcacc ttgagcgtcg gtttgccctg
     3361 cgtcagggtg gccagcatcg ggttggcgct tgagcccggc aggcgcgcgt cgagcagttc
     3421 aacgatcacg tcgatgtgct tgatgcgctc actgatcgcc ttttgcgtca ggtgcatgtg
     3481 gccgggaaac cattgaatgg ccatcgctac aagtccagtg aaggccgcaa cgcggacact
     3541 ttgacaaaac gccagcggcc cacaaaacag gccatgaccg cagcgacaac gccagccaac
     3601 acaaccagtt gcagcggatg ctgccaatcg ccctcaaagg caccaaacag actggctttg
     3661 atggctttaa caacccaggt aatcggcaag taggggctga tgcgggcaaa caggccgccg
     3721 ctcaactcaa ccggcaatac gccacccgag gacgacaact gcacagccag caaaaccagc
     3781 gccagcgcct tgccgccgtc gccgaagacg cgtatcagcg caaagatgat cagcagaaac
     3841 gtcagcgatg caacaccgac ggtcagcgcc agcgccagcg catcaaccgc ttgaacctcc
     3901 agaatccaga ccaccgttac ccataccaac agcgcctgca gtagggcaat gtgcattggg
     3961 atcagcattt ttccgagcag tcgggcaagc ggggacatct gcgcggcaaa caacggcaac
     4021 aggcgcgcct gaatcaaaaa gaccgccaga ctggccccca gccagagtgc acccggaacg
     4081 atgttggggg caaaaccgct gccgttgttt aacacccgag cgtcaatttc catgaccggc
     4141 tgcaccgatg tggccagacc ctgcgcattg ccgtccattt tttttaccga tgctggcaat
     4201 gcatcttcaa gcagttccag cccaccggcc agatgctgtg ctgccagtga cagcttttgg
     4261 gtgccggttt tcaaggcgct ggcaccgctg ctcaggctgg cgctgccacg cgccagttcg
     4321 ctgagtttgc tgtcttcggg caactgggtc accatgctgc gcaaaccatt gttcaaactg
     4381 cgcaagccgg tggtcagggt tgccacaccg gtgttgagct gggtgctgcc gtcagccagc
     4441 ttttgttggg cggcgctggc atttttcaga ccagtctcca gggtcgatac cccctcgccc
     4501 aactgattga gcccctcctt gacccgggtg gcaatgaacg tgctgctgct ggcttcttca
     4561 cgaaaaccgt tgacaccggt ctgcaaacgc tgggtccctt gctgcaattg cagcaagcca
     4621 cccttgagtt cctcgtgccc gtccaccagt gcctgtgcgc cttcgtcgag gcggcgcaac
     4681 tcggagtttt tcggtcgctg cccaaacatg ctgcgcagcc cgccaccaag ttcacgcaca
     4741 ccgctggtca actgaccgat gccctggtcc agtttgcccg agccgtttgc caggccttgc
     4801 gcaccaagcg ccgactgttg cacgccgctg gcgagctccc tggcacccaa acgcagttgc
     4861 tgtacccctt gatgcaactg tgccatgctg cgctgtgagc cggccgcatc ggccagcacc
     4921 agtgcccagc gctgctcgtt aaggctttga ttgacttcac ggcccaggtc ttcggcaaaa
     4981 cgccttgcca aaccggcgct gggatagcta ttgccctctg aggcaaagac caccagcttg
     5041 ccggcacctg cctcggcccc aggtacggca ttggagctga agtccaaagg cacgatcagg
     5101 gcaaacgcca gttcaccctg gcgcaccagg cggcgcgcct gctgttcatc ggtgtagtcg
     5161 acgtagccaa aggcgggttt ggacttcagc cgaccggcca ggtcttggcc gacattgaag
     5221 acgtgttggc ggtactcaaa accatggtca aggttgacga tcgccaccgg caaggcaccg
     5281 gtttttgccg ccgggtccca aacgctggtc aggtagatca gcacgtaaag cgccggaatc
     5341 agcacgacca cagcggcccc cagcagcagc ttggggtagt gcagaaaaaa aagcgcctca
     5401 atccgggcaa tgcggacaaa agtgacgatc tggcccacct caggtctcat gccgcaccgg
     5461 tgcgcgctgg gccttaaggc ctgatcgctc ggtcttttgg gcccgaagtc gttgtcgcga
     5521 ggccctggtt ggcttggtcg gacgccgcgt tttgggtgcc cgggccacgc tgttgaccag
     5581 ttcttgcaag cgcaccacgg catcaaaacg gttcatctcc tgggtgcggt gctgctgcgc
     5641 cttgatcacc acaacgccgt cctggctgat gcggctgtcg cgcaacgcca gcagccgggt
     5701 cttgacatcg ccgggcaagc tgctcgacaa aacgtcaaag cgcaaatgga tggcgcttga
     5761 aaccttgttg acattctgcc cgccggcacc ctgagcccgg atgggcttga gctcaagctc
     5821 gtcatcacgc acctgcagca gctcggtcat ggtcattggg ctggtcctga atcaggccgc
     5881 tgcggcctgc gactccggtg cactgacgta gcgcgccaca aaggcaccca cgctgctctg
     5941 gggcaagggc agccagaccg tgtggccact gccgggtgcc actgacatcg gctcggcatc
     6001 ctggttttcg atgtggctga gctgcacatc gacattgcct gcaggggcaa tgatttccag
     6061 ccagtcgccc acggcaaagc ggtttttgac ctgaacttgt gccagacctc gcacggcatc
     6121 aaagctgagc acatcaccca cataaaggct gcggccggac tcggagtagc cgcgcaggta
     6181 gttttgcgtc gcctctggcg tatggcgctg aaaaaagccg ctggtgtagc cacggttggc
     6241 caggccctca agctggccca gcaaggccgg atcaagctcg cgcccggcga gtgcgtcgtc
     6301 gatggcgcgg cggtagcttt gcacggtgcg cgccacgtaa tacgggctct tggtgcggcc
     6361 ttcgatcttc agcgaatcaa cccctattgc gaccagccgc tggacgtgct caatggcccg
     6421 caaatcccgc gagttcatca cgtaggtgcc gtgctcgtcc tcctcgattg gcatgtagct
     6481 gccgggacgc tggccctctt cgagcagata gacctcattg gcctcgctga cccgctcaag
     6541 tccctctgtc tggcaatcgg caacgcgctg caactcggcc tggttgcgct gaaaagccgt
     6601 ttgcggggcc aggatgtcgc cggacgcatc gacgacagct ttttgcgtct tgtagtccca
     6661 gcggcacgaa tttgtgcaac tgccctggtt ggcgtcgcgg tggttgaagt agcccgacag
     6721 caggcagcgt cccgaatagg caatgcacag cgcgccatgc acaaacactt caagctcggt
     6781 gtcagggcag tcctggcgaa tttgctcaac ctgatcgagt gacagctcgc gcgacaagat
     6841 cacccgactg accccgacgg ttttccagaa acgcaccgca gcggcattga ctgtgttggc
     6901 ctgcaccgac aggtgaattt ccatgtcggg ccaggtctcg cgcaccatca tgatcaggcc
     6961 agggtcggac atgatcatgg catcgggttt gagggcaatc accggtgcca tgttgctgac
     7021 gtaatgtttg atcttgttgc cgtgcgggaa gatgttggac accagaaaaa acttggcccc
     7081 cctggcgtgc gtggtgtcaa tgccctgctg caacacctgc aggtcgccaa aatcgttgtt
     7141 gcgtacccgc aaagagtagc gtggctggcc cgcgtaaaca gcgttggcac caaatgccaa
     7201 cgctgtttcc agcatcttca aggagccagc gggtgccaat agttcaggcg gtttcatgtt
     7261 tgatggcggg ttcaactgag ggagttgggt tcaagggctt ggcatgcttc catgatgcta
     7321 tattttaaat agctgcatac gcaatatctg taagggcttg agcccgatct tg
//
LOCUS       DAOSLL010000164         7354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9262_length_7354_cov_5.990547, whole genome shotgun sequence.
ACCESSION   DAOSLL010000164 DAOSLL010000000
VERSION     DAOSLL010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7354
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9262_length_7354_cov_5.990547"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            800..1630
                     /locus_tag="PLB25_08895"
     CDS             800..1630
                     /locus_tag="PLB25_08895"
                     /inference="COORDINATES: protein motif:HMM:NF024257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOE41730.1"
                     /translation="MKFGQLFDSFRNISWVLASTLALLVVPAHADELTLGGTGNSLGT
                     LRLLGVAFSKKYPDTKVTVMSSLGSSGALKAVPKGALDIGVTSRTLTDEERKSNMAAT
                     EYARTATVIAVSNKSSVTDITREQLADMLTGKLGKWPDGGVIRPVLRQPGDDNSRQLR
                     GLSPAIEQALAKAEQRQGLPFAVTDQEAADKIESIPGAVGVTTLALIKSENRNLRALK
                     LDNVAATPTEAANGKYPLIKHFYFITKVVPSGSVEKFIAFTNSPAGRDILIQTGHWLP
                     "
     gene            1662..3119
                     /locus_tag="PLB25_08900"
     CDS             1662..3119
                     /locus_tag="PLB25_08900"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HOE41731.1"
                     /translation="MSASSSNSYQQIDLIVTRWAASIALVLAVVLPLGFALNAYKNLA
                     DDLEFKAQVKASALNDLIANSPKVWVFAENRMQGLLSREPVPLDNELVEVFDLTHNVL
                     VQSGKPPAQPVLRKSVDLFDADAVVGHLAVTGSMRTLLLETLIATLLSVLLSLSVFLV
                     IRELPLRALRKATDALIDEKDRAQAMLQSISDAVITSDAQGVLMQVNPAGIRIMGAQS
                     VQQMVGHPVADWITPEYRASYADHHQRVLAGQTLPLEYEVLSLRGERRWLESQDTPLR
                     EGGKIVHLAVARDVTERKMAEQEIKSLAFYDPLTGLPNRRLLADRIDQAKADGARTKK
                     YTALLFIDLDKFKAINDSLGHATGDLLLQQVAQRLVSCVREGDTVARVGGDEFVVLLL
                     GLNQELPDARAQTEAVGKKILTSLNTPYQLGQHTHTNTPSIGITLFNDHQYTNNELIR
                     CADLAMYQAKASGRNTMLFFDPGMLTAAARRDDQS"
     gene            complement(3326..3547)
                     /locus_tag="PLB25_08905"
     CDS             complement(3326..3547)
                     /locus_tag="PLB25_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41732.1"
                     /translation="MHETGLRNGATARLFQRKADPRQGTRHRQTDHTGPDHHTIDLFH
                     RPDPTDQTTRAQLARLGLSLGSGNAGSGG"
     regulatory      3696..3933
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4012..5898
                     /locus_tag="PLB25_08910"
     CDS             4012..5898
                     /locus_tag="PLB25_08910"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOE41733.1"
                     /translation="MNSFALSMRTGAPVRLCASVVAVSAALVALPAGAQSQSPVTLGE
                     VVVTATRVEQSLANVLADVTVIEAEAIARSGAVSLADVLVHVPGIEMVRNGGPLGTTS
                     LYLRGGNTNHTVVLIDGIRLDTQNGSGGATWQSIPAQQIDHIEILRGPAAAIYGSDAM
                     AGVIQVFTKKGQSGFQPSLGFGFASFGTRTATAGLLGGFEGWRYALSLGHETSDGYNI
                     QPAANPDKDDYTRDTATLRLGRDLVAGHKLDATWLYTDADAGYDSTTSQSNVNYAKDN
                     RTVQKLNALGMNWRAKWSDVWSSTVGVTQALDHYQTLPSPGAVPSYDTDTTIQTALWH
                     NTLRWRDAIWTVGLEGRRDALTNSGTTPKDTKRNQNALALGYGDQTDAHNWQVNTRLD
                     QDSDFGDHATGTLAYGYKWSPQWRLSASMGTGFRSPTLYQRHSDYGFAGLKPEESFNR
                     ELGVTYRHEAQTLSVVAYHNRISNLIAFGAAGACISTFGCYANTAKATLQGVTFSGEA
                     RWASTLWSMSLDLQDPLDANTGKRLARRAQRLFKLAAETSWQGWDWRADVLLSGDRFD
                     DAANTTRLPGYGLLNVGASRSITRQTELLLRLDNLGDRAYETAKGYANPGRTLFVGLK
                     WTGL"
     gene            5883..7298
                     /locus_tag="PLB25_08915"
     CDS             5883..7298
                     /locus_tag="PLB25_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HOE41734.1"
                     /translation="MDRPVSDTTPKVAALLVAAPASGQGKTTVVAALARLHARQGRTV
                     RVFKCGPDFLDPYWHALASGAPVYQLDLWINGEADCRARLAAAAAQADLIIVEGVMGL
                     YDGDPSAADLAQRFGLPVLAVIDAAAMAGTFGALAFGLQHYRAGLPWAGVLANRVANG
                     QHAQMLQGSVREPECWLGALMHNAAWSLPERHLGLTVASEVHDALARLDAAADALTST
                     PLGQMTLADWQRWRVGFEPSATLPQPAPGPASAGLSGQGDASMPPLQGQTIAVARDAA
                     FCFIYAANLDCLRDLGAELVFFSPLADVALPACDALWLPGGYPELHATTIASNSALRD
                     SLAAHIAAGKPVWAECGGMMALFDTLITADGQRHAQWGLVPGCVTMHKRLAALGPQQL
                     QLASGLLRGHTFHYSTTETALPPVTRTARPDTSAQPDAGEALWQHGSVRASYFHAWFA
                     SCPAAVVELFSAPSGPPGLPA"
BASE COUNT         1448 a         2189 c         2220 g         1497 t
ORIGIN      
        1 cccgttttga acctgttgtg ggccatggct gttcgccaaa ggcgaacctc attttcccag
       61 cttgtctggg aaaatgccta gtctctaaca agtcaagtct ttgcatatca tgcgaagaat
      121 tcaaacattc aaagtcattc gccgcgaggg cgcgactcct acaaattcaa attggtgccg
      181 actgtttgtg taggagtcgc gccctcgcga cgaaggctca tcaattccgg ctcgtccggc
      241 ataggatgtg gtcaggaata acttccccta tttgcaccgg cggatttggg cgcacagcgt
      301 cgttgcaaga cgcttgttta gccagttgca cggcgcgtct tgcgcctagc tgtgcatccc
      361 aactccttgg tgcaaaacgg ggagttattc ctgaccacat ccttaaaggc attttgccta
      421 tgacgaaatc atcgccgggt tcattttgac gcatcagaat tgttcgcagg atgtgtgaag
      481 actgaacttt ctggaaactg aatgaccctg ccgtcaggcg catttttgtt ggtgacttgc
      541 ccaggaaaca cgctatcgcg agaatcatca gggtcggcac ctgagttgtt accacctctg
      601 ccgggcgata cctggactca tgtggttgaa aagcctgtcc gttcagacag tttagtagcc
      661 tgactccatt cgagcctgtt attgtgttac gtcaattccg ccccagggga tgcacaaaat
      721 aatttggttg gaccacctgc tcaagttgcc agcaaactga taactgcgcg cattgtttga
      781 ccgactcaaa ggcataaaaa tgaaattcgg acagcttttt gactcttttc gaaacatttc
      841 ctgggtcctt gcatcaaccc tcgcattgct ggttgtgcca gcccatgccg acgaactcac
      901 gcttggtggc accggcaaca gcttgggcac gctgcgtttg ctgggtgtgg ccttcagcaa
      961 aaaatacccc gacaccaagg tcaccgtcat gagcagcctg ggcagcagcg gcgcgctgaa
     1021 ggcagtgcca aaaggtgcgc tggacattgg cgttacatcc cgtaccctga ctgacgaaga
     1081 gcgcaaaagc aacatggcgg ccactgaata cgcccgcaca gccacggtca ttgcggtatc
     1141 caacaaatcc agcgtgaccg atatcacccg cgaacagttg gcagatatgc tgaccggaaa
     1201 gctcggcaaa tggcctgatg gcggggtcat tcgccctgta ttgcgacagc ctggcgacga
     1261 taactccagg cagctcagag gcttgtcacc cgccattgag caggccctcg ccaaggccga
     1321 acagcgccag ggtctgccat ttgccgttac cgatcaggaa gccgcagaca agatcgagtc
     1381 gatccccggt gccgtcgggg tcaccaccct ggcgttgatc aagtcagaga accgcaacct
     1441 gcgggccttg aagctcgaca acgtggcagc cacaccaact gaggcagcca atgggaaata
     1501 ccctctcatc aaacactttt atttcatcac caaggtggtc ccgtctggca gcgtcgaaaa
     1561 attcattgcg tttaccaact cgcccgccgg gcgtgacatc ctgatacaaa ccgggcactg
     1621 gttgccctga gcctgcacgc caggttccct ctggatatct catgtctgca tccagttcaa
     1681 acagctacca gcagatcgac ctgatcgtga cgcgctgggc cgccagcatt gcgctggtgc
     1741 tggcggttgt gctgccgctg ggctttgcgc tcaatgctta caagaatctg gctgacgacc
     1801 tggagttcaa ggcccaggtg aaggcatcgg ccttgaatga cctgatcgcc aacagcccca
     1861 aggtatgggt gtttgcagaa aatcgcatgc aggggctgct ttcgcgcgag ccagtgccgt
     1921 tggacaacga actggttgaa gtttttgacc tgacccacaa tgtgctggtg caaagcggca
     1981 agccgccagc gcaaccggtg ctacgcaaaa gcgttgacct gtttgatgcc gacgccgtcg
     2041 ttgggcacct tgcggtgacc gggtccatgc gcaccctgct gctcgaaacg ctgattgcca
     2101 cgctgctcag tgtgcttttg agcctgtcgg tctttctggt cattcgggag cttccgttgc
     2161 gcgcgctgcg aaaggcaacg gatgcactga ttgacgaaaa agaccgcgct caggcaatgt
     2221 tgcagtccat cagcgatgcc gtcatcacca gtgatgcgca gggcgtgctg atgcaggtga
     2281 acccggccgg gattcgcatc atgggtgccc agtcagttca gcaaatggtg gggcacccgg
     2341 tcgccgactg gatcacccct gagtaccgcg ccagctatgc tgaccaccac caacgggtgc
     2401 tggccgggca gacattaccc ctggaatacg aagtgctgag cctgcgcggt gagcgccgct
     2461 ggctggagtc ccaggacacg ccattgcgcg agggtggcaa gattgtgcat ttggcggtgg
     2521 cccgcgacgt taccgagcgc aagatggccg aacaggaaat caagagtctg gctttttatg
     2581 acccgctgac cggtctgccc aaccgtcgct tgctggctga ccgcattgac caggccaaag
     2641 ccgatggcgc ccgcaccaaa aaatacactg cactgctgtt catcgacctt gacaaattca
     2701 aagccatcaa tgacagcctg ggtcacgcca cgggcgacct tctgctccag caggtggcgc
     2761 agcgtctggt gtcatgcgtg cgcgaaggcg acaccgtggc gcgtgtcggt ggtgatgaat
     2821 ttgtcgtgtt gctgctaggc ttgaaccagg agttgcctga tgccagagca cagaccgagg
     2881 cggtgggcaa aaaaattctg acctcgctga acacacctta ccagttaggc cagcacacgc
     2941 ataccaatac accgagcatt ggtatcaccc tgttcaatga ccaccaatac acaaataatg
     3001 agctgatcag atgtgccgat ctggccatgt accaagccaa agcgtcgggg cgaaacacca
     3061 tgctcttttt cgatcctggc atgctgaccg ctgccgccag gcgagatgat caaagttgag
     3121 gaccatcaaa accatggtga gattcgctca ggccttggct gactcgacag cgtcaagact
     3181 cattcgacac catcaaacca tcagtccccg tgttgccgcc gtttttacat aaccaattgg
     3241 cctctagcgc ccgtcctcat tgcgcaacta gctattatag ctatagcaac tgtgcagcca
     3301 ccttgatgac tggcccgctc cttttttagc caccggaacc cgcattgcca gaccccagcg
     3361 acagccccag cctggccagt tgcgcccggg tcgtttgatc cgttgggtcg gggcgatgga
     3421 acaggtcgat tgtgtggtga tcggggccgg tgtgatcggt ctggcggtgg cgtgtgccct
     3481 gacgcgggtc ggctttgcgt tgaaaaagtc ttgcagttgc gccattgcgc agccctgttt
     3541 cgtgcatgtt gatcaccata ttggacgatc aacctgctgg cgatttggtg ggctacaggc
     3601 cccgctttgg gctcattcct gggtggaaat acacgcgccg ttcaggctca cacctcgttg
     3661 gacaaggtac ccgggcaggc tgccgtcagg gccagcttag aatactacgc gttggtgctc
     3721 gcggtgtgat tcacacgccg cagttcaacg ggaagcagga gggtcaggcc gaccaagccg
     3781 aacctaacct gcgctgcccc cgcaacggta agtggacgaa ccttgacagg ttccgctgtg
     3841 atcaaggcca ctgggtgttt taaccaacac ctgggaaggc gatgacggta gcctccatca
     3901 gcccggatac cggccaacac ggtggctgtg cgcctgcaaa tgcgtgcaac cgtgacgctc
     3961 atgcactgtc ggggacgatg gtgagagctc cttgatggtt cttttcctac gatgaattca
     4021 tttgctttgt cgatgcgcac aggcgcaccc gtgcgcctgt gcgcgtctgt tgtggccgtt
     4081 tcggcggctt tggtggcttt gcctgcaggg gcgcaaagcc agtcgccggt gacgctgggg
     4141 gaggtggtgg tgacggcgac gagggttgag cagtcgctgg ccaatgtgct ggctgatgtg
     4201 acggtgatcg aggccgaggc tatcgcgcga agtggtgcgg tttcgctggc tgatgtattg
     4261 gtgcatgtgc ctgggatcga gatggtgcgc aatggcggtc ctctgggcac gaccagcctg
     4321 tatcttcggg gtggcaacac caatcacacc gtggtgctga ttgacggtat ccggctcgat
     4381 acccaaaacg gcagtggcgg cgcgacttgg cagtcgattc ccgcccagca gattgaccac
     4441 atcgaaatcc tgcgtggtcc cgctgcggcg atttatggct ccgatgccat ggccggtgtg
     4501 atccaggtgt ttaccaagaa gggtcagtcc gggtttcagc ccagtctggg ctttggcttc
     4561 gcaagcttcg gcacgcgtac cgccacggcg ggtttgttgg gtgggtttga gggctggcgc
     4621 tacgcgttgt ctttgggtca cgaaaccagc gacggctaca acattcagcc cgccgccaac
     4681 cctgataagg acgactacac cagagacacc gcaactttgc gcctggggcg tgatctggtc
     4741 gccgggcaca agctcgatgc tacctggctg tacaccgatg ccgatgccgg ttacgacagc
     4801 accacaagcc agagcaatgt caactacgca aaagacaacc gcaccgtgca aaagctcaat
     4861 gcattgggga tgaactggcg cgccaagtgg tcggatgtct ggtcctcgac ggttggtgtg
     4921 acgcaggcac tggatcacta tcagaccttg ccaagtcctg gggcggtgcc aagttatgac
     4981 accgacacca ccattcaaac cgcactgtgg cacaacacgc tgcgctggcg cgatgcgatc
     5041 tggaccgttg ggctggaggg gcgtcgtgat gccttgacca actctggcac cacgcccaaa
     5101 gacacgaaac gcaatcagaa tgccctggca ctgggttacg gcgatcaaac ggatgcccat
     5161 aactggcaag tcaatacccg actggaccag gacagcgact ttggcgacca cgccaccggc
     5221 acgctggcct atggctacaa atggtcgcct caatggcggc tgtctgcatc aatgggcacg
     5281 ggctttcgtt cgcccaccct ttaccagcgc catagcgact atggctttgc cgggttgaag
     5341 ccggaggagt ccttcaaccg cgaactcggg gtgacttacc gtcatgaggc acagaccttg
     5401 tcggtggtgg catatcacaa ccggatcagc aatttgattg cctttggtgc tgccggtgcc
     5461 tgcatcagca cgtttggctg ttacgccaac accgccaaag ccacgctgca gggggtgaca
     5521 ttcagcggcg aggcgcgctg ggcatccacc ctgtggagca tgtcgctgga cttgcaagac
     5581 ccgcttgatg ccaacaccgg caagcgcctg gcgcgccggg cgcaacgcct gttcaagctg
     5641 gctgccgaaa cctcctggca gggctgggac tggagggccg atgtgttgct gtcgggtgac
     5701 cgctttgacg atgcggccaa cacaacccgt ttgccagggt atggcctgct caatgtcggg
     5761 gccagccggt cgatcacgcg gcaaaccgag ttgctgctgc gcctggacaa tctgggcgac
     5821 cgggcatacg aaaccgccaa gggctatgcc aacccagggc gcaccctgtt tgttggattg
     5881 aaatggaccg gcctgtgagc gataccacac caaaggttgc ggcgctgctg gtggccgcgc
     5941 ccgcctccgg ccagggcaaa accacggtgg ttgccgcgct ggcgcggctg cacgcgcgcc
     6001 agggcagaac agtgcgggtg ttcaagtgcg ggccggattt tctcgacccc tactggcacg
     6061 cgctggccag cggcgcgccg gtttatcagt tggacttgtg gatcaacggc gaggccgact
     6121 gccgcgcccg gctggcagca gcggcggcgc aagccgacct gatcatcgtc gagggcgtga
     6181 tgggcttgta tgacggcgac cccagcgcag ccgacctggc acagcgcttt ggcctgccag
     6241 tgctggcggt gattgatgcg gctgccatgg ccggtacttt tggcgcgctg gcgtttgggc
     6301 tgcagcacta ccgcgctggc ttgccctggg ccggtgtgct ggccaaccgg gtggccaacg
     6361 ggcagcatgc gcagatgctg cagggcagtg tgcgcgaacc cgagtgctgg ctgggcgcgc
     6421 tgatgcacaa cgctgcctgg tcgctgcccg aacggcatct gggcctgacc gtggccagcg
     6481 aggtgcatga tgcgctggcc cggctggatg ccgctgctga tgcgctgacc agcacgccgc
     6541 tgggccagat gacgctggca gactggcagc gctggcgcgt tgggttcgag ccgtcagcga
     6601 cattgccaca acccgcgcct ggccccgctt cggccggtct gtcgggccaa ggcgacgcgt
     6661 cgatgccgcc cttgcaaggc cagaccatcg cggtggcgcg cgatgctgcg ttttgcttca
     6721 tttacgccgc caacctcgac tgcctgcgcg atctgggtgc cgaactggtg tttttctcac
     6781 cgctggccga tgtggcgctg cccgcttgtg atgcactgtg gcttcccggc ggctaccccg
     6841 aattgcacgc caccacgatt gccagcaaca gcgcactgcg tgacagcctg gccgcgcaca
     6901 ttgctgcggg caagccggtg tgggcggaat gcggcggcat gatggcgctg tttgacaccc
     6961 tcatcacagc cgatggccaa cgccatgccc aatggggcct ggtgccgggt tgcgtcacca
     7021 tgcacaaacg gctggccgcg ctcgggccgc agcagcttca actcgccagc ggtttgctgc
     7081 gtggccacac ctttcactac tccaccaccg aaacagcgct gccgccagtg accagaacgg
     7141 cgcgcccgga caccagtgcg caacccgatg cgggtgaggc gctgtggcag cacggcagcg
     7201 tgcgggccag ttactttcat gcctggtttg catcttgccc ggcagcggtg gtagagcttt
     7261 tttccgcccc aagcggccca ccaggcttgc ctgcatgaac ctaagccgga caagccggaa
     7321 ttggacggcc ttcgtcgcga gggcgcgact ccta
//
LOCUS       DAOSLL010000165         7351 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9267_length_7351_cov_4.383361, whole genome shotgun sequence.
ACCESSION   DAOSLL010000165 DAOSLL010000000
VERSION     DAOSLL010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9267_length_7351_cov_4.383361"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..837)
                     /locus_tag="PLB25_08920"
     CDS             complement(16..837)
                     /locus_tag="PLB25_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE41735.1"
                     /translation="MNLSVDILAASILIVDDQQSNVQLLEQLLRETGYTQVSSTMNPE
                     TVCALHRTHRYDLILLDLQMPVMDGFAVMESLKTNVEDDYLPVIVLTAQPGHKLRALL
                     AGAKDFISKPFDLVEVKTRIHNMLEVRLLYKKLASYNELLEATVAERTAELRESQARY
                     RSLAELACDWYWEQDENGAFTKVSGPVLDMLGLRVPSLRGDDDQLLPSGWNDDERMKL
                     HEIIGNRQPFLDFVLHRANPDGYQQVFQVSGEPMFDRLGRFVGYRGIGLETSVKS"
     gene            complement(1011..1592)
                     /locus_tag="PLB25_08925"
     CDS             complement(1011..1592)
                     /locus_tag="PLB25_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019920923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOE41736.1"
                     /translation="MNNFPIVCVGGSAGGLDAYIRLLKNLPPDMGVAIVIVNHITIMA
                     TQLHEVLPRFSSMPVELITDNLLVQPNRVFIIPANRDLHVEGGVFHLKPISKPHGWPD
                     VITVFLRSLAAHWGGQLIAVIVSGYDGDGAAALCSIREAGGITIAQKPSTAIQPDMPD
                     SAIDSGCIDHVLSPEDIAQTIVRIASGNQTVAL"
     gene            complement(1589..2962)
                     /locus_tag="PLB25_08930"
     CDS             complement(1589..2962)
                     /locus_tag="PLB25_08930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HOE41737.1"
                     /translation="MSIPLSDLPDDVLTVNALKLLRRRAEDNPKMRAVLPAQLVNEMT
                     PDDAQLLFHELRVHRVELELQNEELRRAQLELEEARQRYFDLYDLAPVGYCSVTDQGL
                     IIEANLTAAALLGVPRQELVNRSLTRFMAPSHRQIYRDCCRQLHSGGEAQSCELQVSR
                     VVGGTLWLSLTVNMTKDATGTTLLRVMFKDVTERKLLDAVLLQTSHDLELARQAADKA
                     NQAKSDFLADMSHELRSPLNAILGFAQLLDAGTPAPTPAQKSSIDQIIQGGWHLLALV
                     NEILDLASIESGQVALTMGVQSLGEVLQECQLLIAPLALANHIRLEFPSFGEPCWVQA
                     DRHRLKQLLINLLSNAIKYNRTDGSVVVRWQLCPMQRVRVSLEDSGQGLTPAQLAQLF
                     QPFNRLGQESGQQQGSGIGLVVSKRLAEMMGGSIGVSSTPGVGSVFWFELALADAGAD
                     ADAGADA"
     gene            complement(3276..5003)
                     /locus_tag="PLB25_08935"
     CDS             complement(3276..5003)
                     /locus_tag="PLB25_08935"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE41738.1"
                     /translation="MMPAAVELLDAHILVVDDKPANVVLLAQLLAHVGYRKVSSTSDP
                     AAVCALHRTHQYDLILLDLQMPGMDGFEVMAGLREIETGGYLPVLAITVQAKHKLRAL
                     KAGARDFIVKPFYLIEMETRIKNHLEVRLLYKELAKAVSALESYALHDALTGLPNRRL
                     LLEQLALVRGAGVQSAKHGALMFLDLDHFKQLNDTLGHEFGDVLLQQVSQRLQGCLRQ
                     GDSVARFGGDEFVVLLDWLDPQPATAAAQAQAVAQQILAALDLTYNLQGHAYANTISI
                     GVVIFQGEAESISNLLKKADLAMYRAKSLGRNRASFFDASMQAEVQARDTLLHDMRCG
                     LDAGEFLLHYQLQMDAAGSPVGAEALLRWQHPQHGLMLPGQFLELAQDSNLILPLSQW
                     VLRTACAQLLQWAKSPQTADWTLAINVGACQLTQADFVQQVLCTLQASGAPATRLTLE
                     LTEGTLLHDVEDVIDKMNALRSAGVRLCLDDFGVGFTSLGYLRRLPLAQIKLDQAMVH
                     AASSDSSVAVLARAIVDLGASLGVPVIAEGIETAAQRDYCAALGCAAFQGNYFAAPAV
                     VADLPVKIT"
     gene            complement(5000..6649)
                     /locus_tag="PLB25_08940"
     CDS             complement(5000..6649)
                     /locus_tag="PLB25_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE41739.1"
                     /translation="MQNAIFNSANFSSIATDALGVIQIFNVGAERMLGYTAAEVMNSI
                     TPADISDPLEVIARAKSLSLELNTHINPGFEALVFKASRGIEDIYELTYIRKDGSRLP
                     AVVSVTALRDQDEAIIGYLLIGTDNTARRAAEVERERLDQVLRDKNTELEMARALADK
                     ANLAKSEFLSSMSHELRSPLNAILGFAQLIESGMPVLTPTQKASIAQILSAGWYLLDL
                     INEVLDLALIESGRLSLSCEAVLLSEVLQDCQTLVEPLALKKEVQLHYPRLTASRFVS
                     ADRTRLKQVLINLLSNAIKYNRQGGSVHVTCLERGIGHLRICVQDTGNGLVAEKVNQL
                     FQPFNRLGKEDSDEEGTGIGLVVSKRLVEQMGGEIGVISTPGTGSVFWIELNMTNAPK
                     LDETVVETLVTGTESSLGNVRVRSVLYVEDNRANMELVAQLIARRPDLRLFGAEDATR
                     GIALAREHHPDVILLDINLPGISGLQALKILKEDPDTRLIPVLALSANAMPRDIERGL
                     TAGFCRYLTKPIRVAEFMQALDVALEQAQTQRADVATMDQS"
     gene            complement(6677..7105)
                     /locus_tag="PLB25_08945"
                     /pseudo
     CDS             complement(6677..7105)
                     /locus_tag="PLB25_08945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
BASE COUNT         1404 a         2266 c         2184 g         1497 t
ORIGIN      
        1 ggtggtgaat tgggatcatg atttgacgct ggtttccagt ccgatgcccc gatagccgac
       61 aaagcggccg agccggtcaa acatcggctc gccgctgacc tgaaaaacct gctgatagcc
      121 atccgggttg gcccggtgca gcacaaagtc cagaaacggc tgccggttgc caatgatctc
      181 gtgcagcttc atgcgttcat cgtcgttcca gccactgggc agcaactgat catcatcgcc
      241 acgcaacgag gggacccgca ggccaagcat gtcgagcacc gggccagaca ctttggtgaa
      301 tgcgccgttt tcgtcctgct cccagtacca gtcgcaggcc aattcggcca ggctgcggta
      361 gcgggcctgg ctttcgcgca attcggcggt gcgctcggcc acagtggctt ccagcagctc
      421 gttgtagctg gccagctttt tgtacagcag gcgcacttcg agcatgttgt ggatgcgggt
      481 tttgacctcc accaggtcaa acggtttgct gatgaaatcc ttggccccgg ccagcagcgc
      541 acgcagcttg tggccgggct gggcggtcag cacaatcacc ggcaggtaat catcttcgac
      601 attggttttc aggctctcca tcacggcaaa accgtccatc accggcattt gcaggtccag
      661 caagatcagg tcgtagcggt gcgtgcggtg cagggcgcac accgtctcgg ggttcatggt
      721 ggagctcacc tgggtataac cagtctcccg cagcaactgc tcgagcaatt gcacattgga
      781 ctgctggtca tccactatca aaatgctggc ggccaagata tcaacagaga ggttcatcgg
      841 gtattccttg tgcctacttg aaggcctttt cactacaaat ttaggagcta tcagcgcatt
      901 atgtgtaagg gctaaagggc ttttttacat atatttcgca ggtgctcgcc gtgatcctcg
      961 tcaagcgccg atgcttgctc attgcgtcgg ccccgtctgt gcgctggcac tcataaggca
     1021 acagtctgat tgccgctggc gatgcgcaca atggtctgcg caatgtcctc tggcgacagc
     1081 acatggtcaa tgcaaccgga gtcgatggca ctgtcgggca tgtcgggctg gatcgcggtg
     1141 ctgggttttt gcgcaatggt gatgcctccg gcttcgcgga tgctgcacag cgcagccgcg
     1201 ccatcaccgt cgtagcccga cacaatcacg gcaatcaact ggccgcccca gtgggccgcg
     1261 agcgagcgca aaaacacggt gatcacatcc ggccagccgt gcggcttgga gattggcttg
     1321 aggtgaaata cgccgccttc cacatgcaga tcgcgattgg ccggaatgat gaacacccgg
     1381 ttgggctgca ccagcaggtt gtcggtaatc agctcgactg gcatactgga aaagcgcggc
     1441 agcacctcat gcaactgggt tgccatgatg gtgatgtgat tgacgatcac aatcgccaca
     1501 cccatatcgg gcggcagatt tttcagcaag cgaatgtagg catccagccc acccgcagag
     1561 ccgcccacgc agacgatcgg aaagttgttc atgcgtctgc gcctgcgtct gcgtctgcgc
     1621 ctgcgtctgc cagcgccagc tcaaaccaga acacgctgcc aacgcccggc gtgctggaaa
     1681 caccaatgct gccacccatc atctcggcca gacgtttgct caccaccagc ccgatgccgc
     1741 tgccctgctg ctggccagac tcctgtccga ggcggttgaa cggctgaaac agttgcgcca
     1801 actgcgccgg tgtcaggccc tggccgctgt cttccaggct gacgcgcacc cgctgcatcg
     1861 ggcacaactg ccagcgcacc accacgctac cgtcggtgcg gttgtatttg atggcgttgg
     1921 acagcaggtt gatcagcagt tgcttgaggc gatgccgatc agcttgcacc cagcagggct
     1981 cgccaaagct tggaaattca aggcggatat ggttggccag cgccagcggc gcgatcaaca
     2041 actggcattc ttgcagcacc tcgccgagcg actgaacacc catcgtcagc gccacctggc
     2101 ctgactcgat gctggccagg tccagaatct cgttgaccag cgccagcagg tgccagccgc
     2161 cttgaatgat ctggtcaatc gacgactttt gtgctggagt tggtgctggc gtgccagcat
     2221 cgagcagttg ggcaaagccc agtatggcgt tgagtggcga gcgcagttcg tgactcatgt
     2281 cggccagaaa gtcggatttg gcttggttgg ccttgtcggc cgcttgccgg gccagctcaa
     2341 gatcatggct ggtttgcagc agcaccgcat cgagcagctt gcgctcggtg acgtctttga
     2401 acatcacccg cagcaacgtg gtgccagtgg catctttggt catgttcacg gtcaggctaa
     2461 gccacagcgt gccgcccacg acccggctta cctgcagctc gcaactttgc gcctcgccac
     2521 cgctgtgcaa ctgccgacag cagtcgcggt aaatctgccg gtggctcggt gccatgaagc
     2581 gggtcagcga ccgattgacc aactcctggc gcggcacccc caatagcgcc gctgcggtca
     2641 gattggcctc gatgatcagg ccctgatcgg tcaccgagca gtagcccacc ggtgccaggt
     2701 catacaggtc aaagtagcgt tgccgtgcct cctcaagttc caattgcgcg cgacgcaatt
     2761 cctcgttttg cagttccagc tcaacccggt gcacgcgcag ttcatgaaac agcagttggg
     2821 catcgtcggg ggtcatctca ttgaccaatt gcgctggcaa caccgcgcgc attttggggt
     2881 tgtcttcggc gcgccgacgc aacagcttga gggcattgac ggttagcaca tcgtcgggca
     2941 gatcgctaag aggaatcgac atattctggc tcgggggttg gtttagtgtg ctttgggact
     3001 gcgtgatcgc atgcctgggg gcctgtccaa gttatggcgc atcgcgtgcg aaccgacctc
     3061 agcggtccat tttgcaccgg cgctttgacc tgcagccggg tggtgggcca gcaaatccgg
     3121 cacctctggc cggactagcg tcccactgcg ccatctatca gggtagcaga ctcctgccgc
     3181 ctgccaatcg gcaacgcagt gttgttccgc ggcttcaaga tgctctgata aatatagcat
     3241 gcagcgcaat ctggataagg gtttgaatgc tattttcatg tgattttgac gggcaagtcg
     3301 gcaaccactg ccggggcggc gaaatagttg ccctgaaagg cagcacaacc cagcgcagca
     3361 cagtagtccc gctgggcggc ggtctcgatg ccctcggcaa tcaccggcac acccaggctg
     3421 gcacccagat caacaatggc gcgcgccagc accgccacgc tggagtcgct gctggcggca
     3481 tgcaccatcg cctggtccag cttgatttgc gccagcggca gccgtctgag gtagcccagc
     3541 gaggtaaagc cgacaccaaa atcgtccaga caaagccgca ccccggcgct gcgcaatgcg
     3601 ttcatcttgt cgatcacgtc ttccacgtcg tgcagcaagg tgccttcggt cagttccagc
     3661 gtcaggcggg tagccggtgc cccgctggct tgcagggtgc aaagcacctg ttgcacaaaa
     3721 tcggcctgcg tcaactggca ggcaccgacg ttgatcgcca gggtccagtc ggcggtttgt
     3781 gggcttttcg cccattgcaa cagttgggca caggcagtgc gcaataccca ctgcgacagt
     3841 ggcaggatca gattgctgtc ttgcgccaac tccagaaact ggcctggcag catcagtccg
     3901 tgctgcggat gctgccagcg cagcagcgcc tcggccccta ctggcgagcc agcggcatcc
     3961 atctgcaact ggtaatgcaa caagaattcg cctgcgtcaa gaccgcagcg catgtcgtgt
     4021 agcagcgtgt cacgggcttg cacctcggcc tgcatggaag cgtcaaagaa gcttgcccgg
     4081 ttacgcccca gcgacttggc acggtacatc gccaagtcgg cttttttcag caggttgctg
     4141 atcgattcgg cctcgccctg aaaaatcacc acgccgatgc tgatggtgtt ggcgtaggcg
     4201 tggccttgca ggttgtaggt caggtccagc gccgccagaa tctgctgcgc caccgcctgt
     4261 gcctgagcgg cggctgttgc tggctgcggg tccagccaat cgagcagcac cacaaactcg
     4321 tcgccgccaa agcgtgccac gctgtcacct tggcgcaggc agccttgcag gcgctggctt
     4381 acctgctgca gcaagacatc accaaactca tggccgagcg tgtcgttgag ctgtttgaaa
     4441 tggtccaggt cgagaaacat caaggccccg tgcttggcag actgcacccc ggcgccgcgt
     4501 accagcgcca gttgctccag cagcagacgg cggttcggca ggccggtcag ggcatcgtgc
     4561 agcgcatacg actccagcgc gctgacggcc ttggccagct ctttgtagag caggcgcact
     4621 tccagatgat ttttgatgcg ggtttccatc tcaatcaaat aaaacggctt gacgataaag
     4681 tctctggcgc cggccttgag agcgcgcagc ttgtgcttgg cctgcaccgt gatggccaat
     4741 accggcaggt agccgccagt ctctatctcg cgcagcccgg ccatcacctc aaagccgtcc
     4801 atgccgggca tttgcaagtc cagcaggatc aggtcgtact gatgggtgcg gtgcagcgcg
     4861 cacaccgccg ccgggtcgct ggtggagctg accttccgat agccgacatg cgccagcagt
     4921 tgcgccagca acaccacgtt ggccggtttg tcgtccacca ccagaatgtg ggcatccagc
     4981 agttcgaccg cagcgggcat catgactggt ccatggtcgc aacatcagcg cgctgggttt
     5041 gcgcctgttc cagtgcgaca tcgagcgcct gcatgaactc ggcaaccctg attggcttgg
     5101 tcaggtagcg gcaaaacccg gcggtcaggc cacgctcaat gtcgcgcggc attgcattgg
     5161 cgctcagggc cagcaccgga atcaaccggg tgtcagggtc ttccttgagg attttcagcg
     5221 cttgcaggcc gctgatgccg ggcagattga tgtcaagcaa gatcacatcg gggtgatgct
     5281 cgcgcgccag cgcaatgcca cgggtcgcat cttcggcacc gaacaggcgc aggtcgggtc
     5341 ggcgtgcaat cagttgtgcc accagttcca tattggcacg gttgtcttcc acatacagca
     5401 cgctgcgtac cctgacgttg cccaggcttg actcggtgcc ggtcaccagc gtctcaacga
     5461 cggtttcgtc aagtttcggc gcgttcgtca tgttcagttc tatccagaag acgctgccgg
     5521 tgcccggcgt gctgatgaca ccgatttcgc cgcccatttg ctccaccagc cgcttgctta
     5581 ccaccagccc gatgccggtg ccttcttcgt cgctgtcttc cttgccgagt cggttgaacg
     5641 gctgaaacaa ctggttcacc ttttctgcaa ccaagccatt accggtgtct tgcacacaga
     5701 tgcgcaggtg gccaatgccg cgctcaagac aggtcacatg cacgctgccg ccctggcggt
     5761 tgtatttgat ggcgttggac agcaggttga tcagcacctg cttgagccgg gtgcggtctg
     5821 cgctgacaaa gcgggacgcg gttagcctgg ggtaatgcag ttgcacctct tttttcagcg
     5881 ccagtggctc gaccagggtc tggcagtctt gcagcacctc ggacaacagc accgcctcgc
     5941 acgacaggct caagcggccc gactcaatca gcgccaggtc cagcacctca ttgatcaggt
     6001 ccagcaagta ccagccagcg ctcaggatct gcgcgatgct ggccttttgc gtcggcgtta
     6061 gcaccggcat acctgactcg atcaactggg caaagcccag aatcgcgttc agcggcgaac
     6121 gcagttcgtg gctcatgctc gacaagaatt cagatttggc caggttggcc ttgtcggcca
     6181 gcgcgcgggc catctccagc tcggtatttt tgtcgcgcag cacctggtca agccgctcgc
     6241 gctcaacctc ggcggcgcgc cgggcggtgt tgtcggtgcc aatcagcaga tagccaatga
     6301 tggcttcgtc ctggtcgcgc agcgccgtca ccgacaccac cgctggcagg cggctgccat
     6361 ccttgcggat gtaggtcagc tcgtaaatgt cttcaatgcc gcgggatgcc ttgaacacca
     6421 acgcctcaaa gccggggttg atgtgggtgt tgagctcaag gctgagcgac ttggcgcggg
     6481 caatcacctc tagcgggtca gatatgtcgg ccggggtgat gctgttcatc acttcggcgg
     6541 cggtgtaacc cagcatgcgc tcggcaccga cattgaaaat ctggatcacc ccgagcgcat
     6601 cggtggcaat gctggagaag ttggcgctgt tgaaaatggc gttttgcaaa gtggcgactt
     6661 tgcgcagtgc ctgctgcgcg gcatattcgg ccgttacgtt gcgaaacacc agcaccgcac
     6721 ccatcacctg cccctgtgca tcgcgaatcg gtgcgcaact gtcggctatg tcgtattcgg
     6781 caccgtcgcg gctgatcagc acggtgtggt tacccaggcc ctgaatgatg cccagcgcca
     6841 gtaccttggc aaccggcacg gcggagggct ctcgggtggc cttgctgatg atgcgaaaca
     6901 ccgcattgac cggctggccc agcgcttgct cgaaggtcca gcccgtcagc ttctgcgcca
     6961 ccgggttgat cagcgtcacc cgcgcctgtg cgtcggtggc aatcaccgca tcaccaatcg
     7021 aattgagtgt gaccgccagc ttctgctcgc catcctgcag ttgccgattg gcctgctgca
     7081 gttgccggtt ggtcacttcc tgcattgtca gcaggtgtct ggtttcctgg tgcccctggt
     7141 cgcggatgcg ttgctgcccc atttgaaccg tccaggaagc cacaaaggca aacaacagca
     7201 gcgccagcaa gcccaagcct tccagccttg ggtgctctga ccagcgcagc aggtaaacac
     7261 cagccaggac cagcagcagt aaggcggcaa gccaaggcaa gtaaccatcc cccggcaaag
     7321 ccgggggctt taagaattgt gagccgctaa a
//
LOCUS       DAOSLL010000166         7325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9298_length_7325_cov_4.285557, whole genome shotgun sequence.
ACCESSION   DAOSLL010000166 DAOSLL010000000
VERSION     DAOSLL010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7325
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9298_length_7325_cov_4.285557"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2115
                     /locus_tag="PLB25_08950"
     CDS             <1..2115
                     /locus_tag="PLB25_08950"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOE41740.1"
                     /translation="STPAFEHDISFSPDGRTLLYASERDGDSDIFEASVPPDRPASFT
                     AAGPVVEKKVINTEGDALFPAYSPDGKRIAYLDNRQSIKVLDPTTGKTVTALKEGQIY
                     SYQDGDMTLKWSPDSRWIVATTGSLVGDLDITLLDASGKAEPVNLSQSGYSDHAPIFT
                     PDSKAVIWDTDRDGLRNADSSGGQSDVYIVHLTQEAFDAFIQARNGLKPAAQPAQTDN
                     TWQPQARGLRHRITRLTPYSLVSIQFKQVQADGEGLILVTAEVPGVMVGYRLNLHTGA
                     LDKVFTRPLSNAIVSANAKGDTLYSADPGSIDRINLAKGSSTSVAYSARLDYDPRGEL
                     AYLFQYFWRMTQLKFYRPDMHGRDWKALRTAHARYLPHMHQWEDFADLLGEMAGELNA
                     SHMAGIYLHKAALADDTASLGFYEDPTHTGLGIRVASVLVGGPADSARSLLKPGAVIL
                     AVDGQAVTSNEQFHGLLNRKQATPVELSVQAASGTGTATQVITPITQNAELELAYDHW
                     VERRKALTAELSKGRLGYVHINAMDSANYQRTVDQVFGESRDKTGLIVDVRFNKGGLL
                     HDQLATLLTGQVIGGFVSRDGISRGRIPLDRWGKPSVLLANASSYSDGSIFPHLYQRM
                     GIGPVIGDRVPGTGTAVWWIYVLDLIKYGIPQLGATDLKSGWFENDETVPSVLVYNDP
                     NVIAAGRDAQLEAAVQALLVGK"
     gene            2218..3015
                     /locus_tag="PLB25_08955"
     CDS             2218..3015
                     /locus_tag="PLB25_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41741.1"
                     /translation="MHPPKNVGLFTKITAASLAASMALTGVPVFAQSPAPSDISDLLD
                     ARGSDGEFQMQSRGYIQHHTSRSGASVYSYWWQSNTKKCVRVRTEDGRYQQIDTVGNA
                     DCNQKDADGMSTGAAVAVGAAVLLGIAALASKSHHREDRNFDERQTADFERGFRDGQY
                     HHPYSNYNDSREYSDGYNKGVDERGYQSNYRPEYRNYQRPSYGGGNWVYCASEEGECR
                     VPYNTRVRFGVHGRFNTKEVNRNVRCTTREFGDPANGERKRCEYDAR"
     gene            3177..5246
                     /locus_tag="PLB25_08960"
     CDS             3177..5246
                     /locus_tag="PLB25_08960"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HOE41742.1"
                     /translation="MAALVVSTPLLASAADANAATLWPIIERPTRAASVLPVGPLGYS
                     PSHMDRSVSPRSDFYAYATGNWLNSLTIPDSETDIGGFALLKTTLKHQLLSITLRAGQ
                     GGYAKGSPEQQVGDYYRAAMNTARQDQLGLDPLKTDLMQAGAASSPLELARFSADQLK
                     KGGAPLVLGWPVTDIKDNTRMVLALMPGGPQLEQAQYTDPAAQRIRDLYLDYTKRMLR
                     QTGESEEVATQHAKTILAMEAELMAPRLTPLQARDPARTYNIMSLAEAQALIPAVDLG
                     EFLKQIGVEPTTRIQIHDLEGMKTLNRLLSTRPKEDLQTLVRWSVLSANAGFLGKPWF
                     DMADEYQRQRDQLVRSEPREEQTVDAIATQLYHPLSQLYVNTYFPESTRREVTEMVEH
                     IRAEFELRLRSNPWLDEPTRKAALEKLSRVDIQVGYPKSWIDFSSIDIRADDHLGNNQ
                     RIAAFNLKRELDKLPLPVKVERFNSPKYTTPTAVNAAYNPTYNGIDITAAIVQPPFYT
                     PGADKAVNYCTIGAVIGHELTHGFDSNGSRYDAVGNLRNWWTPQASNEFKQRTQLLVN
                     QFNSYKVLPDLVQSGTLTLTENTADLGGITLAHAALNRALKGRQLPKVDGMTTDQRCF
                     VAWAQLWTYKARTERIRLLATTDFHALGFLRGFGPLRNMDAFHKAFGTTPGDPMWLAP
                     TKRARIW"
     gene            complement(5716..6105)
                     /locus_tag="PLB25_08965"
     CDS             complement(5716..6105)
                     /locus_tag="PLB25_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41743.1"
                     /translation="MTAKKSVLIVGLRPELINFADPAYSAFPGLDAGKVMAALTADEL
                     NLNALGYDAQLCLTDFGETAETVIRKKLAEKKYDCVLIGAGVRTITPNFLLFEKLINI
                     VHEHAPTARICFNTKPSDTAEAVKRWI"
     gene            complement(7051..>7325)
                     /locus_tag="PLB25_08970"
     CDS             complement(7051..>7325)
                     /locus_tag="PLB25_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465843.1"
                     /note="functions in anaerobic transport of
                     C4-dicarboxylate compounds such as fumarate; similar to
                     DcuA; DcuA and DcuB function as independent and mutually
                     redundant C4-dicarboxylate (aspartate, malate, fumarate
                     and succinate) transporters; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HOE41744.1"
                     /translation="SQAAAISAMVPVGLAIGVPPGYIVAFAAASYGYYILPTYPSDLA
                     AIQFDRSGTTHIGKYVINHSFILPGLIGVSTSCLIGYLLAGLYGLI"
BASE COUNT         1601 a         2166 c         2134 g         1424 t
ORIGIN      
        1 tcaacaccgg cgtttgaaca tgacatcagc ttcagtcccg acggccgtac cctgctgtac
       61 gccagcgaac gcgatggtga ttcagacatt tttgaagcct ccgttccgcc agaccgcccc
      121 gcgtcgttca ccgccgccgg tccggtagtg gagaagaagg tgatcaacac cgaaggcgac
      181 gcgctgtttc cggcgtattc acccgacggc aagcgcattg cctacctgga caaccgccaa
      241 agcatcaaag tgctggaccc gaccaccgga aagacggtca cagcactcaa ggaaggccaa
      301 atttacagct atcaggacgg tgacatgacg ctgaagtggt ctcccgacag ccgctggatc
      361 gtggccacca cgggttccct ggttggcgac ctcgacatca cattgctcga cgcatcgggc
      421 aaggccgagc ccgtcaatct gtcgcaaagt ggttacagtg accacgcgcc catcttcaca
      481 cccgacagca aagcggtgat ctgggatacc gaccgcgatg gtttgcgcaa tgcggacagc
      541 agcggtgggc agtccgacgt ttacatcgtc cacctcactc aggaggcttt cgatgcattc
      601 atccaggcgc gcaacggcct caaacctgcg gcacagccag cgcaaaccga caacacctgg
      661 cagccccagg cgcgcggcct gcgccaccgc atcacccggc tgacaccgta ctcgctggtc
      721 agcattcagt tcaaacaggt ccaagctgac ggcgagggct tgattttggt gaccgcagag
      781 gtgccaggtg tgatggtggg ctaccgtctg aacctgcata ccggtgcctt ggacaaggtg
      841 tttaccaggc cgctaagcaa cgccatcgtc agcgccaacg ccaagggcga caccctctac
      901 agcgccgacc cggggtcgat tgaccgcatc aacctggcca agggcagcag cacctcagtc
      961 gcctacagcg cccggctcga ctatgaccca cgtggtgagc tggcctacct gtttcagtat
     1021 ttctggcgca tgacgcagtt gaagttttac cggcccgaca tgcatgggcg tgactggaag
     1081 gcgctgcgca ccgcgcatgc gcgctacttg ccacacatgc accagtggga agattttgcg
     1141 gacctgttgg gtgaaatggc gggtgaactc aatgcgtcgc acatggctgg catttacctt
     1201 cacaaagcgg ctttggccga cgacaccgca tcgctggggt tttatgaaga cccgacgcac
     1261 accgggctgg ggattcgcgt ggcatcggtt ctggtgggcg gccccgccga cagcgcccgg
     1321 tcgctgctga agccgggcgc agtgattctg gcggtcgatg gtcaggcggt taccagcaac
     1381 gagcagttcc acggtttgct caatcgcaag caagccacac cggtcgaact gagcgtgcag
     1441 gcggcatcgg gcaccggcac ggccacccag gtcatcacac caatcaccca gaatgcagag
     1501 cttgagctgg cctacgacca ttgggttgaa cgccgcaagg cactgactgc cgagctttcc
     1561 aagggccgcc tgggatacgt gcacatcaat gccatggaca gtgcgaacta ccagcgcacc
     1621 gtggaccaag tgtttggtga atcgcgcgac aagaccgggc tcattgtgga tgtccggttt
     1681 aacaagggtg gactgctgca cgaccaactg gcaacgctgt tgaccggcca ggtgatcggc
     1741 ggttttgtca gccgcgatgg catcagccgt ggcaggattc cgctggaccg ctggggcaaa
     1801 cccagcgtct tgttggccaa tgccagcagc tattcggatg gcagcatttt tccgcacctg
     1861 taccagcgca tgggtattgg gccggtgatt ggtgatcgag ttccgggcac cggcactgcg
     1921 gtttggtgga tctatgtgct ggacctgatc aaatacggca ttccccaact gggtgccacc
     1981 gatctcaaga gtggctggtt tgaaaatgac gagaccgtgc ccagtgtgtt ggtttacaac
     2041 gaccccaatg tcattgctgc ggggcgcgac gcgcagttgg aggcagcggt gcaggcattg
     2101 cttgtgggaa agtgaacctg tacccttgga tggctgggcc tgcaggactg ggaaattgaa
     2161 tgtcatgact gatggcagaa taccgtttcc aatttcaact cgccttcagg agatgtcatg
     2221 caccccccaa agaatgttgg cctctttacc aaaatcacgg ctgcatcgct ggccgcatcg
     2281 atggcgttga cgggtgtccc ggtctttgcc caaagcccag cgcccagcga catcagcgat
     2341 ttgttggatg cccggggcag cgacggcgag tttcagatgc aatcgcgtgg ttacatccag
     2401 catcacacat cgcgaagcgg tgccagcgtt tacagctatt ggtggcaaag taacaccaag
     2461 aagtgcgtgc gtgtacgcac tgaagatggt cgctaccagc agatcgacac cgtgggcaac
     2521 gcagactgca atcaaaaaga cgctgacggc atgagcaccg gtgccgctgt ggctgtgggt
     2581 gcggccgttc tgctcggcat tgcggccttg gcctccaagt cacaccaccg cgaagaccgc
     2641 aattttgacg agcgccaaac cgcagatttc gagcgcggtt ttcgcgatgg ccagtaccac
     2701 catccctaca gcaactacaa cgattcgcgt gaatacagcg acggctacaa caaaggggtg
     2761 gatgaacggg gctaccagtc aaactatcgc cccgagtacc gcaactacca gcgaccgagc
     2821 tacgggggtg gcaattgggt gtactgtgca tcggaggaag gcgagtgccg ggtgccctac
     2881 aacacacgcg tcaggtttgg cgtgcatggg cgcttcaaca ccaaggaggt caatcgaaac
     2941 gttcgctgca ctacccgtga gtttggcgac ccggccaatg gcgaaagaaa gcgttgcgaa
     3001 tacgacgcgc gttgatcaag tagagcattg cgctcaaggc agatgtgccg ttccttcagg
     3061 cgggcaattc ggagctggcc aatttggggg gtacgcgcga cagatttcgt tttttttgga
     3121 gtggttgaaa catgttcgct gtgcatcttc cagtgagggg gctgcccctt aaagccttgg
     3181 ccgcgcttgt ggtcagcacg ccactcctgg catcggctgc cgatgccaac gcggcgaccc
     3241 tgtggccaat tattgagcgc cccacccgag ccgcctcggt gttgccagtg gggccactgg
     3301 gctactcccc cagccacatg gacaggtcgg tcagcccacg cagcgacttt tacgcctacg
     3361 ccaccggcaa ctggcttaac agcctgacca tccccgactc ggagaccgac atcggtggct
     3421 tcgcgttgct gaagacaacg ttgaagcacc agttgctcag catcacgctg cgtgctggcc
     3481 agggtgggta tgccaaaggc agcccagagc aacaggtggg cgactactac cgcgccgcca
     3541 tgaacaccgc ccgccaagac cagttggggc tggatccttt gaagactgac ctgatgcagg
     3601 cgggggcagc gtcaagccca ttggagttgg cgcgtttctc ggccgatcag ttaaaaaaag
     3661 gcggcgcccc gttggtgctt ggttggcccg tcaccgacat caaggacaac acccgcatgg
     3721 tgttggcgct gatgcctggt ggtccgcaac tggagcaagc ccagtacacc gacccggccg
     3781 cccagcgcat ccgcgacttg tacctggact acaccaagcg tatgctgcgc caaactggtg
     3841 aaagcgaaga agttgccacc cagcatgcca agaccatcct cgcgatggaa gccgagctga
     3901 tggcaccccg actgacaccc ttgcaagccc gcgacccggc gcgcacctac aacatcatga
     3961 gcttggccga ggcacaagcg ttgatcccgg ccgttgatct gggcgaattt ctcaagcaaa
     4021 tcggcgtcga gcccacaacg cgcattcaga ttcatgatct ggagggcatg aaaacgctca
     4081 accgcctgct cagcacccgc cccaaggaag acctgcaaac cctggttcga tggtcggtgc
     4141 tgtcggccaa cgcgggcttt cttggcaagc cgtggtttga catggccgat gagtaccagc
     4201 gccagcgcga tcaattggta cgttcggagc cacgcgagga acaaacggtg gatgccatcg
     4261 ccacgcagct ttaccacccg ttgtcgcagt tgtacgtcaa tacctatttc cctgaatcca
     4321 cccgccgcga ggtgaccgag atggtggaac acattcgtgc ggagtttgaa ctgcggcttc
     4381 gttccaaccc ctggctggac gaacctaccc gcaaggcggc gctcgaaaag ctgtcgcggg
     4441 tggacattca ggtcggctat ccaaaatcct ggatcgactt cagcagcatc gacatccgcg
     4501 ccgacgacca cctgggcaac aaccagcgca tcgccgcctt caacctcaag cgcgagctcg
     4561 acaaattgcc actgccggtc aaggttgaac gctttaactc cccgaagtac accacgccca
     4621 ctgcggttaa cgcggcctat aacccgactt acaacggcat cgatatcacg gcagccatcg
     4681 tgcagccccc gttttacacg ccaggtgccg acaaggccgt gaactactgc accattggcg
     4741 cggtgattgg tcatgagtta acccacggtt tcgacagcaa cggcagtcga tacgatgccg
     4801 tcggcaattt gcgcaactgg tggacacccc aagccagcaa cgagttcaag cagcgcacgc
     4861 agttattggt caatcaattt aatagctaca aagtattgcc agacctggtt cagagcggca
     4921 cgctgacgct gaccgaaaac accgcagacc tgggaggtat cactttggcc cacgcggcgc
     4981 tgaaccgggc tctcaagggg aggcaactgc cgaaagtgga tggcatgacc actgaccagc
     5041 gctgttttgt ggcgtgggcg cagttgtgga cctacaaggc gcgcacggag cgcattcgct
     5101 tgttggcgac aacagatttc cacgccctgg gttttctgcg cggctttgga ccgctgcgca
     5161 acatggatgc gttccataag gcgtttggca ccacgccggg tgacccgatg tggctcgccc
     5221 caaccaaacg ggcacgcatc tggtaagacg cttggggtgc ctgcctgcac cgccggttgc
     5281 gacatcgacc aacggtgttg gagaaatcac gatctgggtg ccaggtgacc caccaggcat
     5341 tcctgcccaa tacctttgcc gccagatttc cgcgtgtgtt gacaggcatt cacacctgct
     5401 cggtactgtg gcggcgcttg gtcaaaagca gctcaaaacg gctgatttgg ctgcctcaag
     5461 atgcctggtt tggccacaca gccattgcaa tgatggctag acttatcggc gctgccggtc
     5521 ctggggttcc caagaccctt gtagccagag tggtgggaaa agatgttttc agtgcaggtg
     5581 aggcaggcgt ttttggcctg atccgggctc gtccaacaaa tggtgcagat cgtgggcttg
     5641 gcacgaactg accttgctcg ctaggcatcg tctttggcat cgcgcctttg ccacttggcc
     5701 cagtacatat ttgacttata tccagcgctt gacggcctct gccgtatcac tcggcttcgt
     5761 gttaaagcaa attcgcgcgg tcggcgcatg ctcatgaaca atgttgatca acttctcaaa
     5821 caaaaggaaa ttgggtgtaa ttgtgcgcac tccagcgcca atgagaacgc agtcatactt
     5881 cttctcagcg agtttctttc ggatgacagt ttccgccgtt tcaccgaaat cagtaagaca
     5941 aagctgtgca tcgtatccaa gtgcattgag attgagttcg tcggcagtta gcgcagccat
     6001 caccttgcct gcatccagac ccggaaatgc agagtaagct ggatctgcga agttaatgag
     6061 ctcaggccgc agcccgacaa taaggacgct tttttttgct gtcatgagta ctcctttgga
     6121 ttagaaaaac ggcggaaacg gacgggatca ttgaaagggt gtgagcgggt cttttgcgat
     6181 gcctaacgaa taacgtttat cttgcgcatg acaaaactga cgaagacgca caacgcccgc
     6241 cgcccaagac aaaccttgtt ggactgaacc gccgttgcca agcggtagcg acaagttgat
     6301 tgaaagacat cctgatactt tgtgcgcggc tcatggcccg aactttaccg catcgcgcgt
     6361 accagacttt gatcgcatgt ggcaagcggt cgccgccagc cgcaaccagg ctggcggtgt
     6421 gagcgtcaaa ttgaatgcca gcgtggcgct ggttgaggcg agcacaacac cgcccccgcc
     6481 tcgggcgaag tgttcaaaag gacagcactg acgcaccagc tcacgcctta cgcgggcggt
     6541 cgtcgcttgt tgggagccta aacatcacca gggtcaggca agcgttgcga gcccgccatg
     6601 accgccacga cacgcaacgg ccgccgcttg caccgcgtgc acacatccat gtcaaaacca
     6661 gccatgcgcg cgatgaaagc cagcacgctg cagtgccagg acgttgggcg caggcatgtg
     6721 catgacccag ggcgctccga cgggatatga ggtgaggatg acagggccaa tgcaccccaa
     6781 tcaggccggg cgttttctgt gcctcgtcat tgctggagca tcacaatggt atctatttaa
     6841 tagcattcaa cgcagtcatc ataagggcca gaggccaata tgacaccaaa tctgcagtca
     6901 acccgactcc gccatcgatc tggctgaagc tgacgttcag gaactgggtt caatttggtt
     6961 ggccccatgt gcagcttgcc ctcttttcag acagcagcgg ggcaaatcaa cagttcgctg
     7021 gtctggccgt cgattggcca ctgtgcggcc tcaaatcagg ccgtaaagcc cggccagcag
     7081 atagccaatc aggcaggagg tgctgacccc gatcagcccc ggcaggatga agctgtggtt
     7141 gatgacgtat ttgccaatat gggtggtgcc agagcggtca aactggattg cggccaggtc
     7201 gctagggtag gtgggcaata tgtagtagcc ataagaggct gcggcaaacg ccacgatgta
     7261 accgggtggc accccaatgg ccaggcccac cggcaccatg gcgctgatgg cggccgcctg
     7321 tgaat
//
LOCUS       DAOSLL010000167         7324 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9299_length_7324_cov_3.945522, whole genome shotgun sequence.
ACCESSION   DAOSLL010000167 DAOSLL010000000
VERSION     DAOSLL010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7324)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7324)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7324
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9299_length_7324_cov_3.945522"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="PLB25_08975"
     CDS             complement(<1..681)
                     /locus_tag="PLB25_08975"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HOE41745.1"
                     /translation="MERTGHYAVAEQLQADGVEHIFGNPGTVEQGFLDALGDFPSIKY
                     ILTLQESVAIMAADAYARANRKPAVAQIHSSPGLGNAIGALYQAMRGHSPIVVLGGDS
                     GIRYQAMNAQMAADLVAMARPVTKWSTMVQHPSSLLRVLRRAMKVAATPPMGPVYVCL
                     PQDILDALATEDVFPSFIPTSRSQPEAEFIDQAAELLLAASRPMIFVGDGIAYSGAQS
                     ELARVAELL"
     gene            complement(731..1564)
                     /locus_tag="PLB25_08980"
     CDS             complement(731..1564)
                     /locus_tag="PLB25_08980"
                     /inference="COORDINATES: protein motif:HMM:NF028434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HOE41746.1"
                     /translation="MNTEKRVLAVMSEWGYWGIELVGPLNKLVAAGYKFDFVTPKGKR
                     SPVLPPSTDPKYFDPPLGVCVTTQEDADLVTAFEKTNQLDQTKNLSEMFPERPYFSEP
                     DFLRKFEAYYEKRRLAQEKALATYDAILLVGGSGPIVDIVNNQRVHDLILGFNAAKKP
                     IGAICYGVAALVFARDFNERNAIIAGKHVTGHCIEYDYHDGTGFVGTDFNMGPPPYVL
                     EYILADAVGPNGGYHGNFGKRTSVIVDYPFITARSLQCAHEFGDRFVDVLTSDMKRSG
                     W"
     gene            2670..3407
                     /locus_tag="PLB25_08985"
     CDS             2670..3407
                     /locus_tag="PLB25_08985"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE41747.1"
                     /translation="MEQKLRVGITGAGGVLGSALVQGLREKYELVLFDSKPLHNLKQC
                     ESKRLDLADPAQLPGAFEGLDAIFHLAGNPNPGAPHADTMRNNFLATSLTFEQARRDG
                     VKKIVFASSNFFHQKDIAVALRSGRKSSIRLDDRASPDCLYGDSKVYGEHLGQHYANL
                     GIDFVALRIGWSVPGDTPVPYNSQYMRAMFCSKRDLVKAFEQSLESKEKFITAFAISN
                     NDEKVFDLTETQAKFGFYPQDNSAHYF"
     gene            3427..4239
                     /locus_tag="PLB25_08990"
     CDS             3427..4239
                     /locus_tag="PLB25_08990"
                     /inference="COORDINATES: protein motif:HMM:NF012455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HOE41748.1"
                     /translation="MTKVADVPVDQNQALVCLMAHGACEFIATLALLLAVMGSFAWLV
                     VPFVAPALQPLVLATVVGATIALIAKSPIGKLSGAHMNPAISLAFWLQRRMSTSEMLV
                     YVLMQFGGAVTACELLSVILHKHFEAIGFGVLSPASGLSTTGLLAFEAGATAGLVVIL
                     FVVLSRQQLTRHAAISVALYVALVTVWLAPLTGASLNPARAYGAALAAHQLNGLWPYC
                     VAPLAGAVIASVGYGLITWLPKPRYHRLNHSHEHANYTLHLWDRLQRFRRQP"
     gene            complement(4279..4767)
                     /locus_tag="PLB25_08995"
     CDS             complement(4279..4767)
                     /locus_tag="PLB25_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HOE41749.1"
                     /translation="MPHHSLKSTEVVFEEEFVAGLKKVFEELIVFNQVLGLKITSLKP
                     EQVRARINMRNELVGHFTYNRIHGGVISAGLDAMGGLAVMAAIGARHMDESPIQRLQR
                     FAKLGTIDLRVDYLRPGIGDHFEFRAEVMRLGSRVASTRMEFLGADGKLLSTGAAAYI
                     VS"
     gene            complement(5069..5833)
                     /locus_tag="PLB25_09000"
     CDS             complement(5069..5833)
                     /locus_tag="PLB25_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HOE41750.1"
                     /translation="MADLLIDTTGQVMTITFNRVDKKNSFTSAMYADMADALTGAQTD
                     DRVRVAVFQGHETIFSSGNDIADFLKNPPLSLDVPVFRFLRAISTFAKPLVAAVCGPA
                     VGIGTTLLFHCDLVYAGDNAAFSMPFVNLGLCPEAASSLLAPQRMGYGRAAQALLLGD
                     PFMAEAALEMGLVSRIVPPAEASALAQRQAAKLASKPISSLLQTKRFMKLGQSEAVAQ
                     RLAEEGAVFGRMLGEPAAREAFTAFMEKRLPDFSGV"
     gene            complement(5867..7063)
                     /locus_tag="PLB25_09005"
     CDS             complement(5867..7063)
                     /locus_tag="PLB25_09005"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOE41751.1"
                     /translation="MKQLTDAYIVAATRTPIGKSHRGVFRNTRPDDLLVKVLQSALAQ
                     VPGLDPKAIEDVICGCAIPEGQQGLNIARIGAVLAGLPTSVGGITVNRFCASGLSAIQ
                     IAADRIRVGEADVMFAAGVESMSMVPMMGNSPSLSPTMFDNDENIGIAYGMGLTAEKV
                     ANQWKVSREAQDAFAVESHRRAIAAQAAGDFAAEITPIEVTDRAPDLANGTVLERKRM
                     VSVDEGPRADTSLAGLAKLRAVFAARGSVTAGNSSQTSDGAGALILASEKAVKQFGLT
                     PLARYVSFAAKGVPPQIMGIGPIEAIPAALRYAGLKQDDIDWIELNEAFAAQSLAVVN
                     TLGLDPAKVNPIGGAIALGHPLGATGAIRAATVIHALQRKQLRYGMVSMCIGMGQGAA
                     GIFERI"
BASE COUNT         1656 a         2116 c         1952 g         1600 t
ORIGIN      
        1 gagcaactcg gcaacccgcg cgagttcaga ctgagcaccg gaataggcaa tgccgtcgcc
       61 gacaaagatc atcggcctgg atgcggccag aagcaattca gcggcttgat cgataaattc
      121 agcttcgggc tgactacgac tagttggaat aaacgaggga aacacatctt ctgtcgccag
      181 ggcatccaag atatcctgag gcaaacacac ataaaccggc cccataggtg gcgttgcggc
      241 aactttcatg gcacgccgca aaacgcgcaa cagcgacgag ggatgttgca ccatggttga
      301 ccacttcgtg accggtcgtg ccattgctac cagatcggca gccatctgcg cattcatggc
      361 ctggtagcgg atgccagaat caccacccag gacgactata gggctgtgac cccgcatggc
      421 ttggtacaac gcgccgatgg cgtttccaag acctggtgaa ctgtgaatct gcgctaccgc
      481 tggtttgcga ttggcacgcg cataggcatc ggctgccatg atggccaccg attcctgcaa
      541 ggtcaggatg tatttaattg aggggaaatc acccaacgcg tcaagaaaac cctgttcgac
      601 agtacccgga ttgccgaaaa tatgctccac cccatcggct tgaagttgct ctgctactgc
      661 atagtgacca gttcgttcca ttcgaattgc tcctgatttt cgacaatgtc ggcggcaaag
      721 aagaaactaa ctaccagccg gaacgtttca tatcactggt caatacgtct acaaagcgat
      781 ctccgaattc atgggcacac tgcagcgagc gcgcagtaat aaaggggtaa tcgacaataa
      841 ctgaggtacg cttgccgaaa ttgccatggt atccaccgtt cgggccaact gcgtcggcaa
      901 gaatatattc aagcacgtag ggtggtggcc ccatattgaa gtcggtacca acaaagcccg
      961 taccatcgtg atagtcgtat tcaatgcaat ggccggttac atgcttgccg gcaatgattg
     1021 cgttacgctc attaaaatcg cgagcaaaaa ccagcgccgc cacaccgtag caaatcgcgc
     1081 cgattggctt cttcgcagcg ttgaaaccaa ggatcaaatc atgtacccgc tggttgttga
     1141 caatgtcaac aatcgggcca ctccccccaa ccagcaaaat cgcatcgtaa gttgcgagtg
     1201 ccttttcctg ggctaagcgg cgcttttcgt aataggcttc gaacttgcgc aaaaaatcag
     1261 gctcgctgaa ataaggtcgc tcaggaaaca tctcggacag gttcttggtc tgatcaagtt
     1321 gattggtttt ctcaaacgct gttaccaagt cggcatcctc ttgggttgtc acgcaaaccc
     1381 caagcggagg gtcgaagtac tttgggtctg tgctgggcgg caataccggt gaacgctttc
     1441 ccttgggcgt aacgaaatca aacttataac cagcagccac cagtttgttg agcggaccga
     1501 ccaactcaat accccagtag ccccattcag acatgaccgc tagcacgcgc ttttcagtat
     1561 tcattgttgt ctccaaagat ttcgttgact cgattgataa cagtaccagg aacattcgcc
     1621 cgttacaaaa aaattccaac aacacgctgg cagcagagta tcagcaataa aagaaacttc
     1681 gtacacctcc aaatcaaatc tttttattgc acatgttgca tacacccaac aacccatcac
     1741 caccagctac acgtgtcccg cccaccccaa tcgctgtaaa gccacgcatg attggtttag
     1801 ccaaccgcag gtgccgccat agttgatgcg atgtgctcct cagcgactca atgcaactct
     1861 ggttttcacc tcacatcttg cctagcaaga gcggcatgac cgaatctcat gcttgcagca
     1921 gtcacaccca atcaaaccaa caacctgtct gccgaacacg tttatgcggc tttagacgat
     1981 ccaatcaact cctgatgacc agcctgtcaa gcacaacccc agcccctgta gacaagtccg
     2041 tttcgcacca gtctttgcca ccgtcttgac ccgcagcgcc tggctgctgg ttacctctct
     2101 caaccatgag cgccaaaacg cgagaactct ttatcacatt taaacgatga ttgcattgac
     2161 ataacttaaa caagtggcaa ttaaaacgcc actttgtttg tcctagatca atgataggtc
     2221 aatttttttg ggcatgtgtt gcttttttcg gaaatttttg cgttccagtg ttccagcgtt
     2281 gcggcttgcg catgggcctg gtggcagtca tcgggaagcg accgcgtcga gccagtgtct
     2341 tggtacgtgc ccgtaaaccc catctgcttc tgggcagatc ggttcgtttg cgggttgatc
     2401 cacaacgctg cccacgccac ctcgtccgaa ctggttacac cagccgcgtt ggacgctttg
     2461 acggcagcag caaacggtca acaacgtccc tccattggcc tgacaccctg gtgccaacaa
     2521 agatcttggg gtcgttgaac ttgaaacgct tggcctggcc tttcccgccc tggccttcgg
     2581 cctgcaacca tcgccacacg ttgcgccacg taaaatcgaa aagctcaaaa ctctgaaatg
     2641 tgattgaatt gaccattgga ggtattgcaa tggaacaaaa actgcgggtt ggaatcaccg
     2701 gtgctggcgg agttttgggc tctgcgctgg tgcaaggttt acgcgaaaaa tacgagttgg
     2761 tgctgtttga ctcaaagccc ttgcacaatc tcaagcaatg cgaaagcaag cgactggact
     2821 tggctgaccc ggcccagttg cctggtgctt tcgaagggct tgatgccatc tttcaccttg
     2881 ccgggaatcc aaaccccgga gcacctcacg ctgacacgat gcgcaacaat tttttggcta
     2941 ccagcctgac gtttgagcag gccaggcgcg acggtgtcaa aaaaattgtc ttcgcgagct
     3001 ccaatttttt ccatcagaaa gacatcgctg tggcactgcg aagcggtcgc aagtcgagca
     3061 ttcggctgga cgaccgggct agcccggact gcctctatgg cgactcgaaa gtctatggcg
     3121 agcatttggg gcaacactat gccaacttag gtattgactt tgtggcgctt cgcattggct
     3181 ggagcgtgcc tggggatacg ccagtgccct acaacagcca gtacatgcgt gccatgtttt
     3241 gcagcaagcg cgatctggtc aaggcttttg aacaatcgct cgaatcaaag gagaagttca
     3301 tcacagcctt tgccatcagc aacaacgacg aaaaggtgtt cgatttgacc gaaactcaag
     3361 cgaaatttgg cttttatccg caagacaact ctgctcacta tttctagcaa attctggacc
     3421 tcgctcatga ccaaagtcgc cgatgttcct gttgatcaga atcaggctct ggtttgcctg
     3481 atggctcatg gcgcatgcga attcattgca acgctggcgt tgctgctggc cgtgatggga
     3541 agttttgcct ggctggtggt tccatttgtg gccccagccc tgcaacccct ggtgttggcc
     3601 actgtggtgg gcgcaacgat tgcattgatc gcgaaatccc ccatcggcaa gctttctggt
     3661 gcgcacatga acccggcgat ttcactggct ttctggctac agcggcgcat gagcaccagc
     3721 gaaatgctgg tctatgtgct gatgcagttc ggtggcgcag taacggcctg tgaattgttg
     3781 tcggtcattt tgcacaagca ctttgaagcc atcgggttcg gcgttctgag tccggcatcc
     3841 ggcttgtcca cgaccggtct gctcgccttt gaggccggtg caactgcggg cctggtggtg
     3901 atcctttttg ttgttctgag tcgtcaacaa ttgactcggc atgccgccat ttcggtagca
     3961 ctttatgtgg ctctggtgac tgtctggctg gcaccgctga caggtgccag tctcaacccg
     4021 gcgcgtgcct acggggctgc cctggcggct catcagctca atggtctgtg gccgtattgt
     4081 gtggcaccgc tggcaggtgc tgtgatcgcc tcggtcggct atggactgat cacttggcta
     4141 ccaaaacccc gctatcaccg cctcaaccat agccacgaac acgccaatta cacgctgcat
     4201 ttatgggacc ggctgcaacg atttcgtcgc cagccataga tgaagtggcg gggctactag
     4261 agaatcagcc ctgatgcctc aggacacgat gtaagccgcc gctccggtgg acaacagttt
     4321 gccgtctgca cccagaaatt ccatccgcgt ggaggccacc cgcgagccca gacgcatcac
     4381 ttcggcacga aattcaaagt ggtcgccaat accggggcgc aggtaatcca cccgcaaatc
     4441 gatggtgcca agcttggcaa aacgctgcaa gcgctggatc ggtgactcgt ccatgtggcg
     4501 cgcaccaatt gcggccatca ccgccagccc tcccatggca tcaagcccgg cactgatgac
     4561 gccgccatga atgcggttgt aggtgaaatg cccaaccaac tcattgcgca tgttgatgcg
     4621 cgccctgacc tgctcaggct tgagcgaggt gattttcaag cccagcacct ggttgaaaac
     4681 aatcagttct tcaaacacct ttttgagtcc ggcaacaaac tcttcttcga acacaacttc
     4741 ggtggattta agtgaatggt gaggcatgaa atactacaaa aaaaatagct gtacgcgcag
     4801 acggtaaaag ggctggaggc caatacgact taaaagttgc gggcgctgtg ctgagcatct
     4861 gctggatcat ggactttgct gggccgactg taccacccgc gacctgcaac ccgctggatg
     4921 ctggcctgcg gcgagcggga gtttcgccat aggcaaaacc cccaatagct atgaactata
     4981 tagctgctgg cgcaatctgg gcaagggcta gaggcatatt tgtttcaatc agacggttgg
     5041 ccggctgggc tgggatacct tgcatcaatc aaacgccgga gaagtctggc aggcgctttt
     5101 ccatgaaagc ggtaaacgcc tcgcgggccg caggctcgcc cagcatgcga ccaaacacgg
     5161 caccctcttc agccagccgt tgtgccacgg cctcagactg acccagcttc atgaagcgtt
     5221 tggtttgcag cagtgaactg atgggcttgg atgccagctt tgccgcctgc cgctgcgcca
     5281 gcgcgctggc ctcggcgggt ggcacgatgc gactcaccag gcccatttcc agcgccgctt
     5341 cggccatgaa agggtcgccc agcaacagcg cttgggcggc ccgtccataa cccatgcgct
     5401 gcggtgccag caggcttgag gcggcctctg gacacagacc cagattgaca aacggcatgg
     5461 aaaaagccgc gttgtcacct gcatagacca agtcgcagtg gaacagcagt gtggtgccaa
     5521 ttcccaccgc cgggccacac acggcagcca ccagcggctt ggcaaaggtg ctgatcgctc
     5581 gcagaaaccg gaacaccggc acatccagcg acagcggcgg gtttttcaga aagtcggcaa
     5641 tgtcgttgcc ggaactgaaa atggtctcat gcccctgaaa taccgccacc cgcacccggt
     5701 cgtcggtttg cgcaccggtc agcgcgtcgg ccatgtcagc gtacatcgct gacgtgaacg
     5761 aatttttttt gtccacccgg ttaaaggtga tggtcatcac ctgtccggtg gtgtcaatca
     5821 agaggtcagc catgatgttg cccgcaaaag tcgccagaac tctggctcaa atccgctcaa
     5881 aaatgccagc cgcgccctgc cccataccga tgcacatgct gaccatgccg tacctgagct
     5941 gcttgcgctg cagcgcgtgg atcacggtgg cagcacgaat cgctccggtg gcaccgagcg
     6001 gatgacccag cgcaatggcg ccgccgatcg ggttgacctt ggccggatcg agcccgaggg
     6061 tgttgacaac cgccagcgac tgggcggcaa aggcttcatt gagctcaatc cagtcaatgt
     6121 cgtcctgctt caggccggca taacgcagcg cggccggaat cgcctcaatc ggaccaatgc
     6181 ccatgatctg cggcggcacg cccttggcgg caaacgacac atagcgcgcc agcggtgtca
     6241 agccaaattg cttgacagct ttttcgctgg ccagaatcaa ggcaccggca ccgtccgagg
     6301 tttgcgaact gttgccagcc gtcactgaac cgcgcgcggc aaaaacggca cgcagtttgg
     6361 ccagaccggc cagcgaggtg tcggcacggg gtccttcgtc gacgctgacc atgcgctttc
     6421 tctcaagcac cgtgccattg gccagatcgg gcgcgcgatc agtgacttca attggcgtga
     6481 tctcggcggc aaaatcgcct gcagcttgcg cggcaatggc acggcggtgc gactcaacgg
     6541 caaacgcgtc ctgcgcctcg cggctgactt tccactggtt cgccactttt tcagccgtca
     6601 ggcccatgcc gtaggcgatg ccgatgttct cgtcgttgtc aaacatggtc ggcgacagcg
     6661 atggcgaatt gcccatcatc ggcaccatgc tcatgctctc aaccccggcg gcgaacatca
     6721 catcggcctc gccgacacgg atgcggtcag cggcaatctg gatggccgac agacccgagg
     6781 cgcaaaaacg gttgaccgtg atcccgccga cgctggtcgg caggcccgcc agcaccgcac
     6841 caatgcgggc gatgttcagg ccctgctgac cttcaggaat ggcgcagccg cagatcacat
     6901 cctcgatggc tttggggtca agcccaggta cttgcgccaa ggctgattgc agcaccttga
     6961 ccagcaggtc atcaggccgc gtgttgcgga aaacaccacg gtgcgacttg cctatcggcg
     7021 tgcgggtggc ggcaacaatg taggcatcgg taagttgttt catttggata tcctttgatt
     7081 tcttgggaac agatcaacaa ttgctgcgca atggttggtt cgttccgcaa acatctttag
     7141 tcgattgggg acagggcaaa aactgctgcg caattgttgg tctgtcccgc aaacaccttt
     7201 attcagttag ggacagagca aaaattgctg cgcaattgtt ggtctgtccc gcaaacacat
     7261 tagtttctca ccggcttgcc ggttgacatc atgcccatca cccgttcctg ggttttgggg
     7321 tgat
//
LOCUS       DAOSLL010000168         7310 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9315_length_7310_cov_4.514955, whole genome shotgun sequence.
ACCESSION   DAOSLL010000168 DAOSLL010000000
VERSION     DAOSLL010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7310)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7310)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7310
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9315_length_7310_cov_4.514955"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(211..1536)
                     /locus_tag="PLB25_09010"
     CDS             complement(211..1536)
                     /locus_tag="PLB25_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41752.1"
                     /translation="MKIDMHHTLPRPLDPGEAFFFMSDHIACMNFVVLAERNGHLDPS
                     RIQGALDLIQQENLLLQARICCAEEAGLRFESAPGLPIELYCHQASADNWQSWIELEL
                     SRPFAEGTAPLMRCLYLEVAAPACCVLALTFHHAVADGRSGTELLRRTLSLLATDALP
                     PSSGVAAALPAMINVMPTRFRWADQPDAAKLLRNTLIADYRRHGALPPVPWLDIGASE
                     RIPRLIRRSFAADVTRRLISQARAQGTSVHGVLCAAQLLAQLKLRPGDEPGAFFLSCP
                     VDMRAHLEPVPPLVPTGLFVSIVSATFSVTPDTEVWQLAREVTQQTRLQLDRGEGHLF
                     FNMFGLDGTPVLPDRIAPFQKKLMASMPNTMVSNVGAVATVADDPAVESISFALCPMP
                     YQTLFTAASSYKDQLILNVGFDAAKLTEENARILIQGACDILAAAADVA"
     gene            2024..3196
                     /locus_tag="PLB25_09015"
     CDS             2024..3196
                     /locus_tag="PLB25_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOE41753.1"
                     /translation="MAKITVVGAGYVGLSNAVLLARHNEVIVHDIVAQRVAMINLRQS
                     PIEDVELQDHLQRMSLDLLATLDPALAYSKADIVLIATPTDYDSETNHFNTSSVEQVI
                     HDICATSPSALVVIKSTVPVGFTARMREKFNTGQLIFSPEFLREGRALYDNLYPSRIV
                     VGERSQRAQAFAELMLQGAVKKDVTMILTDSAEAEAIKLFANTYLAMRVAFFNELDSY
                     AMGHKLDTRQIINGVCLDPRIGQHYNNPSFGYGGYCLPKDTRQLLANYAEVPQNLIRA
                     VVDANATRKDVVAADIVVRQPGIVGIYRLAMKAGSDNFRASSVQGIMKRIKARGIEVV
                     VYEPALQAQHFYQSRVIADLAEFKQAADLIVANRRTAELDDVAHKVYTRDLFGGDA"
     gene            3287..3895
                     /locus_tag="PLB25_09020"
     CDS             3287..3895
                     /locus_tag="PLB25_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41754.1"
                     /translation="MHTFFDQLAIQRWDDHRYYHHSRVNQSLHFVSAISFLVAYIFLF
                     IDPAIAALIGWLVSMTSRQAGHFFFEPLGYDEVNQATNAHKEAIKVGYNLRRKVVLLS
                     VWLASPLLLLLDPSAWGTLTPHSSVSGFVHNTGRIWLVIGVGGLLFRTLHLFFLKGPM
                     AGLVWMSKILTDPFHDIYLYHKAPLALLRGELIEPRKPLGQA"
     gene            complement(3956..5629)
                     /locus_tag="PLB25_09025"
     CDS             complement(3956..5629)
                     /locus_tag="PLB25_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOE41755.1"
                     /translation="MTEMTSMGALLPYLAGGIGAIWGLARAKQRIELSLAKHRSLAGH
                     PRMARRLAAWLPRYAYTLEQALAIDAAPAEVVAQRLAAVERLGSTFKTRFAKSLGQTA
                     AMANSMSDLQFVGTYRTPFQFKAIVDQGLKIASFVASTEGISVTDLDGNVFYDLTGSY
                     GVNLFGNDFYKHSIDTGADLVRPLGPVLGAYHPVVADNVQRLKQLSGMDEVSFHMSGT
                     EAVMQAVRLARYHSGRNKVVRFCGAYHGWWGDVQPGIGNPMTARDTFTLAEMGDASLR
                     VLRRRKDIACVLINPLQALHPNGAAPGDSSLLDSSRRAGFDRAAYTAWLLALRQICDE
                     RGIALIFDEVFVGFRLAAGGAQAYFGVRADLVTYGKTLGGGLPIGVVCGKHAWMKRFR
                     DERPADICFARGTFNSHPYVMGAMNAFLQYLQSPQGNAQYQDLDGIWNRRAAHLNRAL
                     TQAGVPVQVANLSTIWTVLYTQPACYNWLFQHYLRLHGLALSWVGTGRIIFSLNYTDA
                     DFEAVCTRFVSAALEMQQDRWWWHSGRSNKEIKRRILGELIGARFKRQA"
     gene            5673..6554
                     /gene="asd"
                     /locus_tag="PLB25_09030"
     CDS             5673..6554
                     /gene="asd"
                     /locus_tag="PLB25_09030"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517196.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HOE41756.1"
                     /translation="MQIRKYIHAILANEDINFLLTNRLPRNTLTRFMGWFSKIEQPWV
                     RDASIAAWKFFTDLDLSEAKKTRFTSMHDCFTRELRDGTRPLDDAADTLCSPVDAIIG
                     AHGRIQGTRVFQAKGMPYTLDELLGDHERVEDWQDGSFVTLRLTSSMYHRFHAPYDCV
                     VEQVRYFSGDTWNVNPIALRRVERLFCRNERALIRTTLVGTGPCAGQRLALVPVAAIL
                     VASIRLHFLDVLLHLTYQGPKRFDCQVKLRKGQEMGWFQHGSTVILLAPKGFVMAAGL
                     GEGTRIRMGQALMRWLV"
     gene            complement(6535..>7310)
                     /locus_tag="PLB25_09035"
     CDS             complement(6535..>7310)
                     /locus_tag="PLB25_09035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE41757.1"
                     /translation="YLRKLHNHTQTTMVPTRTLMLDLQNRGYHHVKLLARGVSNTLFC
                     PTRRSPALRRSWGVEDSDLVVLLVGRLAKEKNVGLVITAYRAIASLQPGARLVFVGDG
                     PLRAALQTACPEAIFAGMRQGEDLAAHYASGDLFLFSSLTETFGNVVPEALASGLAVV
                     SYACAAAQELITTDVNGVLVPVGDESQFISAAVRTAQNASNRDALRSAAAPSVAHLGW
                     ESIFDTFVEHLRRVLESQCSGFPIGAPQVAPGYVRQASA"
BASE COUNT         1474 a         2186 c         2199 g         1451 t
ORIGIN      
        1 gatgtggtcg ggaataactc cccgttttgc accaaggagt tgggatgcac ggcttaggcg
       61 caagacgctt gtttagcccg ctaaacaagc gtcttgcaac gacggcgtgc gcccaagtcc
      121 gccggtgcaa ataggggaag ttatttctga ccacatcctt agcgcggcac cgacgccggg
      181 cgcatcaacc aggcaccctg caccagaggc tcaggccaca tccgctgctg cagccaaaat
      241 gtcgcaggca ccctgaatca ggatgcgggc gttttcctcg gtcagtttgg cggcatcaaa
      301 acccacgttc agaatcaact ggtctttgta gctgctggcc gccgtgaaca gggtctggta
      361 gggcatcggg cacagcgcaa acgagatcga ctcgacggca gggtcgtcgg ccaccgtggc
      421 caccgccccc acattgctga ccatggtgtt gggcattgag gccatcaact ttttctggaa
      481 aggcgcgatg cgatcaggca gaactggcgt gccgtccagc ccgaacatgt tgaaaaacaa
      541 atggccttcg ccccggtcaa gctgcaagcg ggtctgctgg gtgacctcgc gtgccagttg
      601 ccacacctcg gtatctggtg tcaccgaaaa ggtggctgaa acaatcgaca caaacaggcc
      661 ggttggcacc agtggcggca ccggctccag gtgcgcgcgc atgtcaaccg gacaggacag
      721 aaaaaacgca cccggctcgt caccgggccg cagcttgagc tgggccagca gttgcgctgc
      781 gcaaagcaca ccgtgcacgc tggtgccttg cgcgcgggcc tggctgatca gccgccgggt
      841 gacatcggca gcgaagctcc gccgaatcag ccgtgggatt cgctcgctgg ccccaatgtc
      901 aagccacggc actggcggca aggccccgtg gcggcggtaa tcagcgatca gcgtgttgcg
      961 caacagcttg gctgcgtcag gctgatcggc ccagcgaaag cgcgtcggca tgacattgat
     1021 catcgcgggc agggcagcgg caacgcccga ggatggcggc agtgcgtctg tcgccagcag
     1081 gctcaaggtg cggcgcagca gctcggtgcc agagcgcccg tcagcaacgg catggtgaaa
     1141 agtcagcgcc agcacacagc aagctggtgc tgccacttcc agatacaggc atcgcatcag
     1201 cggtgcggtg ccttcggcaa agggccgcga aagctccagc tcgatccagc tttgccagtt
     1261 gtctgcgctt gcctggtggc agtacagttc aatcggcagg ccgggtgccg attcaaagcg
     1321 caggccagcc tcctcagcgc agcagatcct ggcttgcagc aaaaggtttt cctgctggat
     1381 caggtcaagc gcgccctgaa tccgggacgg gtcgaggtgg ccgttgcgct cggccagcac
     1441 gacaaaattc atgcaggcaa tgtggtctga catgaagaaa aaggcttctc cggggtcaag
     1501 agggcggggc agggtgtggt gcatatcaat cttcaagggc ttgggcggta gccaccgccg
     1561 gggatgagtt acaggacttc gatgcggccg agcttgtcga gaaagatgac aaaccagcct
     1621 gccgggttgg tcatggctgt cctttcaggg tcagcgccga tcaagcgccg gtaaccgaat
     1681 tagaccgcaa aaaatgccga aaacccggcg cgaccggacc tcaccaatgc ctcgcccaac
     1741 tgcctttggt ccggatcaac tggcctgcat cccagggtac tgggttgccg gggtcgcgtg
     1801 gcatcctggt tctgtcaaac tcgaaataga tccaggttgc aattgggctt tgcgaagcaa
     1861 gccgacaggc ccgatgccca tgcgttgcgg gcgtgaaggt gtgcccgata cttggccggg
     1921 ttatttgtga ctttttcatg acgcttcggg tttgcctgcc aacctgagca gatgtcatga
     1981 gtgcttcaca aaccaaatgc aatatcggca actggaggca cttatggcaa aaatcaccgt
     2041 tgttggcgca ggttatgtgg gcttgtcaaa tgccgttttg ctggcacggc acaacgaggt
     2101 gatcgtgcat gacatcgttg cacagcgtgt cgcgatgatc aatttgcgtc agtcgccgat
     2161 tgaagatgtc gaattgcagg accacttgca acgcatgtcg ctcgatctgc tggcaacgct
     2221 ggaccccgca ctggcctaca gcaaggctga tattgtgctg atcgcaaccc caaccgacta
     2281 cgactcagaa accaaccatt tcaataccag cagcgtcgaa caggtgatac atgacatctg
     2341 tgcaacgagc ccgtcggcac tggtggtgat caagtcaact gtgccagtgg ggtttactgc
     2401 ccggatgcga gaaaaattca acaccgggca gttgattttt tcgccggaat ttttgcgcga
     2461 aggtcgagcc ttgtacgaca acctgtaccc cagccgcatt gtggtcgggg agcgctcgca
     2521 gcgcgcccag gcgtttgccg agttgatgct gcaaggcgcg gtcaaaaaag atgtcacgat
     2581 gatattgacc gacagcgctg aagctgaggc gatcaaactg tttgccaaca cttatctggc
     2641 catgcgggtg gcttttttta acgagctcga cagctatgcc atgggccaca agctcgatac
     2701 ccggcaaatc atcaatggtg tctgccttga cccgcgcatt ggccagcatt acaacaatcc
     2761 gagctttggt tacggtggct attgcctgcc caaggacacc cggcagttgc tggccaacta
     2821 cgccgaggtg ccgcaaaacc tgattcgggc ggtggttgat gccaacgcca cgcgcaaaga
     2881 cgtggtggcc gccgacattg ttgtgcgcca acccggcatc gtgggcatct accgtcttgc
     2941 catgaaagcc ggtagcgaca attttcgcgc cagcagcgtg caaggcatca tgaaacgcat
     3001 caaggctcgg ggcatcgagg tggtggtgta cgagccagcg ttgcaagcgc aacactttta
     3061 tcagtcgcgg gtgattgccg acctggccga gttcaagcag gcagccgacc tgatcgtggc
     3121 caaccggcgc accgccgagc ttgacgacgt ggcgcataaa gtctacacgc gagacctgtt
     3181 tggcggtgat gcctgaggtt tgcccgcttt catgcggatg tcacagcccc tgtcacgttt
     3241 ctgccatttt tctccgctcc aatggtgcgc aaaggagaac tttgtcatgc acactttttt
     3301 cgaccaactg gccattcaac gctgggacga tcatcggtac taccaccaca gccgagtcaa
     3361 ccagtccttg cactttgtca gtgccatcag ctttctggtg gcctacatct ttttgttcat
     3421 tgatccggcc attgcggcac tcatcggttg gctggtgtcg atgaccagcc gtcaggcggg
     3481 ccacttcttt tttgagccgc tgggctatga cgaagtcaat caggccacca atgcgcacaa
     3541 ggaagccatc aaggtcggct acaacttgcg ccgaaaggtg gtgctgctca gtgtctggct
     3601 tgcctcgcca ctgttgttgc tgctcgatcc cagtgcctgg gggaccttga caccgcacag
     3661 ctcagtgagc ggctttgtgc acaacaccgg caggatctgg ctggtgattg gtgtcggtgg
     3721 tctgctgttt cgcacgctgc acctgttttt cctgaaggga ccaatggcag gactggtctg
     3781 gatgagcaag atccttaccg acccgtttca tgacatttac ctctatcaca aggcaccgct
     3841 ggcgctgctg cgtggcgagt tgattgagcc acgcaagccc ctcggtcagg cctgaggctt
     3901 tgcggcgcac cggctttgcc gcttcttctg cggcgcttgt caggtgcgca gtgtgtcagg
     3961 cctgccgttt gaatctggca ccgatgagtt cgccgagtat ccggcgtttg atctctttgt
     4021 tgctgcgtcc actgtgccac caccacctgt cttgctgcat ctccaaagcg gcgctgacaa
     4081 atcgggtgca gaccgcctca aagtcggcat cggtgtagtt caggctgaag atgatgcgcc
     4141 cggtaccaac ccaactcaac gccaggccgt gcagacgcag gtaatgctga aaaagccagt
     4201 tgtagcaggc gggctgggtg tacagcacgg tccagatggt cgacagattg gccacctgaa
     4261 ccggaacgcc ggcttgggtc aaggcgcgat tgaggtgcgc cgcgcggcga ttccagatgc
     4321 cgtcaaggtc ctggtactgc gcattgccct gagggctttg cagatactgc aaaaaggcat
     4381 tcatcgcgcc catcacatac ggatgcgagt tgaaggtgcc gcgcgcaaaa cagatgtcgg
     4441 ctggtcgctc gtcccgaaaa cgcttcatcc aggcgtgttt gccacacacc acgccaatcg
     4501 gcaggccgcc gcccagggtc ttgccgtaag tcaccaggtc ggcgcgcaca ccaaaatatg
     4561 cttgtgcacc gcctgcggcc agccggaaac ccacgaatac ctcgtcaaag atcagcgcaa
     4621 tgccgcgttc gtcgcagatc tgacgcagcg ccagcagcca ggcggtgtag gcggcccggt
     4681 caaaaccggc ccggcgactg ctgtcaagca gcgacgaatc acctggcgcg gcaccgttgg
     4741 ggtgcagcgc ctgcagcggg ttgatcaaga cgcaggcaat gtctttgcgc cggcgcaaca
     4801 cccgcagcga agcgtcaccc atttcagcca atgtgaaggt gtcgcgcgcg gtcatcgggt
     4861 tgccgatgcc gggctgcacg tcgccccacc agccatggta ggcaccgcaa aaccgcacca
     4921 ccttgttgcg cccgctgtgg tagcgcgcca gccggaccgc ttgcatcacc gcctcggtac
     4981 ccgacatgtg aaagctgact tcatccatgc ccgaaagttg cttgagcctt tgcacgttgt
     5041 ccgccaccac cgggtgatag gcacccagca ctggcccaag cgggcgcacc aggtcggctc
     5101 cggtatcaat gctgtgtttg taaaagtcgt tgccaaacag gttgacgccg taagagccgg
     5161 tcaggtcgta aaacacgttg ccatccagat cggtgacgct gatgccctcg gtgctggcga
     5221 caaaagacgc gatcttgagc ccttgatcga cgatggcctt gaactggaac ggcgtgcggt
     5281 aggtgccgac aaactgcagg tccgacatgc tgttggccat agcggcggtc tgccccaggc
     5341 ttttggcaaa gcgggtcttg aaggtcgagc cgagccgctc aactgctgcc agtcgctgcg
     5401 ccaccacctc ggctggtgca gcatcgattg ccagcgcctg ctccagggtg taggcatagc
     5461 gaggcagcca ggccgcaagc cgacgcgcca tacgcgggtg tccggccagc gagcggtgct
     5521 tggccagcga caattcgatg cgctgcttcg ccctggcaag cccccagatc gcgccgatgc
     5581 cgcctgccaa gtaaggcaac agtgcaccca ttgatgtcat ttcagtcatc tcaattcacc
     5641 ttttttttga ccccaccggg acgtgtctta ccatgcagat tcgcaaatac attcacgcca
     5701 tcctggccaa cgaagacatc aatttcttat taaccaaccg attgccgcgc aacaccttga
     5761 cgcgcttcat ggggtggttc agcaagattg agcagccctg ggtgcgcgat gcctcgattg
     5821 cggcctggaa gttttttacc gatctggatc tgagcgaggc caaaaagacc cgcttcacca
     5881 gcatgcacga ttgctttacc cgcgagttgc gcgacggcac acgcccgctg gacgatgccg
     5941 ccgacacact gtgcagcccg gtcgatgcca tcatcggcgc ccatgggcgc attcagggca
     6001 cgcgggtctt tcaggccaag ggcatgccct acacccttga tgaactgttg ggcgatcacg
     6061 agcgtgttga agattggcag gacggcagct ttgtcaccct gcgcctgact tccagcatgt
     6121 accaccgttt tcacgcaccc tacgactgcg tggtcgagca ggtgcgctac ttttccggcg
     6181 acacctggaa cgtcaatccg attgccctgc gccgggtgga gcgattgttt tgccgcaacg
     6241 agcgggcgct gatccggacc acccttgtgg gcaccgggcc atgcgcgggc cagcgcctgg
     6301 ccctggtgcc ggtggcggca attctggtgg ccagcatccg actgcacttt ctcgatgtgc
     6361 tgctgcatct gacataccag ggccccaagc gctttgactg ccaggtcaaa ctgcgcaaag
     6421 gacaggaaat gggctggttt cagcatgggt caacggtcat cttgctggcc cccaaggggt
     6481 ttgtgatggc tgccgggctg ggggagggca caagaatccg gatgggccag gcgcttatgc
     6541 gctggcttgt ctgacatagc ccggcgctac ctgtggggca ccgatgggaa agccgctgca
     6601 ttggctctcc agtacacgcc gcaggtgttc cacaaaggtg tcaaaaatag actcccagcc
     6661 cagatgcgcc acactgggtg ccgctgccga gcgcagagcg tcgcggttcg aggcgttctg
     6721 ggctgtgcgc acagcggcgc tgataaattg cgactcgtcg ccgactggta ccagtacgcc
     6781 gttgacatcc gttgtgatga gttcctgcgc ggcggcacag gcatacgaca ctaccgctaa
     6841 cccgctggcc agtgcctcgg gcaccacatt gccaaaggtt tctgtcaggc tggaaaacag
     6901 gaacaagtca ccgctggcgt aatgtgcggc caggtcttcg ccctggcgca tgccggcaaa
     6961 aatcgcctca gggcaggcag tctgcagggc cgcgcgcagc gggccgtcgc caacaaacac
     7021 cagtcgcgcg ccgggttgca agctggcgat ggcgcggtaa gccgtgatca ccaatccaac
     7081 atttttttct ttcgccagcc gtcccaccag caaaaccacc aggtcgctgt cttccacgcc
     7141 ccaactgcgg cgcaacgcag gtgagcgcct ggtcgggcaa aacagggtgt tggagacgcc
     7201 acgcgccagc agcttgacat ggtggtaacc gcggttttgc aggtccagca tcagggtgcg
     7261 cgtcggcacc atggtggtct gtgtatggtt gtgcagcttg cgcaggtagt
//
LOCUS       DAOSLL010000169         7290 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9344_length_7290_cov_3.532550, whole genome shotgun sequence.
ACCESSION   DAOSLL010000169 DAOSLL010000000
VERSION     DAOSLL010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7290)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7290)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7290
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9344_length_7290_cov_3.532550"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..661
                     /locus_tag="PLB25_09040"
     CDS             92..661
                     /locus_tag="PLB25_09040"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOE41758.1"
                     /translation="MKDAANNAFLALFRDYSEEGVRKYIAADIIQHNPFVPIGRDALI
                     GFSLTLKKMGITYTNHRILRDGEFVVLHNSCNNAQAFGAPKIVTIDIYRMKAGQAVEH
                     WDATAPLAEKTASGRSQVDGATEITDIDKTTANKALAVSLIEDVLMGKNPSKITEYIS
                     AEQYHQHNPGIKDGLSGIVEAEAQMSAAA"
     gene            651..911
                     /locus_tag="PLB25_09045"
                     /pseudo
     CDS             651..911
                     /locus_tag="PLB25_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829035.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
     gene            complement(1203..2165)
                     /locus_tag="PLB25_09050"
     CDS             complement(1203..2165)
                     /locus_tag="PLB25_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HOE41759.1"
                     /translation="MHLKHSGIRPFKQVDVFTATPYLGNPLAVVLDGTGLSDAEMQRF
                     AAWTNLSETTFVLRPTPEAAAAGADYQVRIFTTAYEMPFAGHPTLGTCHAWLEAGGVP
                     KDPARIVQQCKVGLVAMARQGQRLAFAAPPLQRNAPSDALWNALPAALGLAPAQLRAA
                     QHQINGPHHFGVLLDSAESVLAVQPNLAQLQALMAAYGFSGLGVAARHDTAAQVRLIG
                     RSNREARAFGARTTNAAAAAPDLEVRFFFDRGHGVGEDPITGSFNASLAQWLIADGHA
                     PPSYVVAQGTCIGREGRVHITQDAAGQVWVGGDVVTCVDGTVSL"
     gene            2435..2638
                     /locus_tag="PLB25_09055"
     CDS             2435..2638
                     /locus_tag="PLB25_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41760.1"
                     /translation="MRPGSLSTQCNVCGSPGWYCKPDSSVKHARYCQGNFTRQGNSNT
                     KYVKKEDLPEVRKQLKNYQRMKL"
     gene            complement(2683..3342)
                     /locus_tag="PLB25_09060"
     CDS             complement(2683..3342)
                     /locus_tag="PLB25_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE41761.1"
                     /translation="MEPGKERILIVDDDVTNIMLLKAALWHEYDIDTATGGQEAISRV
                     QEHPPELILLDVMMPDLSGFDVCSIIKSSEKLAHIPIIFLTGMDTLDGEIRGLEAGGI
                     DYLTKPVNIELVKLRVRNHLELNRRSEIIRQQRDLLEVRKQELEATLARMKRLEGIIH
                     ICMRCKKIRDDGDSWRQLEQYISEHTDALFSHGLCPSCCQDEMKLDSAKSSPLFRRFL
                     G"
     gene            complement(3682..4212)
                     /locus_tag="PLB25_09065"
     CDS             complement(3682..4212)
                     /locus_tag="PLB25_09065"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE41762.1"
                     /translation="MHGSINVAATMMERHGNNVLLHLAEQDTGIGMAAKILEQIFMLF
                     AQAEVPIARKHGSAGLGLAICRRLVELIGGRFHVKSREGAGRMSHRLQTFQVVDLAQF
                     VQPQSDLPPTAWDGLALRIFLVEDNAIYNRCAMALLGRLGHQPGQICSFAKSLIYRHS
                     RADGNLVQSGFRPARE"
     gene            complement(4495..6036)
                     /locus_tag="PLB25_09070"
     CDS             complement(4495..6036)
                     /locus_tag="PLB25_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE41763.1"
                     /translation="MKLFDLKIGARVALAFSVVLLTIVLLVVAVQVGLFRSAANSQAM
                     GDGVRVQAQATEMHLLAKDNAIASMVILVSSSADLQTKLTKEIAERDAHIAKDMDDLA
                     KALAGSEEDSALLVEVRKRHKNYVAGVQRIVGMVKAGKQAEAAFAADEEMIPMLAPFM
                     TSLEKLNARQVSNVQDKGAANSSLIAGTRMIASIAGALAVVLAVAAGLLLVRSIIRPL
                     RHAVDFAEAVSQGDLTQKVDTTGNDEIAVLMRALDHMQNNLSAVVQKVRQGSDSVASA
                     SAEIASGNMDLSNRTEAQAGALERTDASMAELGEAVKQNAESTRQANQLALQASSVAV
                     KGGEVVGQVVQTMQGINASSRKIADIIQVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVASEVRSLAGRSAAAAREIKALITASVERVEHGSVLVDHAGVTMTEVVNSIKRVTD
                     LMGEISTANNEQSTDVSQVAEVVMQMDMSTQQNAALVEQMAAAAGSLRSQAQDLVQTV
                     AVFKL"
     gene            complement(6089..6499)
                     /locus_tag="PLB25_09075"
     CDS             complement(6089..6499)
                     /locus_tag="PLB25_09075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41764.1"
                     /translation="MKKWFAIVAFAVLGFGATSAVLANEFEDLRTEMTAARESLVTLV
                     VNKDKRGADQQKLVKETADKVSAHLAKMKAPAGKEAQFKELSEIWAAFKKTRETELVP
                     AVLAGKDDDAKKLVNGIQKERYTKCQQLVGELGG"
     gene            complement(6574..6741)
                     /locus_tag="PLB25_09080"
     CDS             complement(6574..6741)
                     /locus_tag="PLB25_09080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41765.1"
                     /translation="MSAKPDGVALTIIEDQAYRDEVCALVKAGIPVIAYKVDDSKGAK
                     GSCSDSCGESP"
     gene            6801..6974
                     /locus_tag="PLB25_09085"
     CDS             6801..6974
                     /locus_tag="PLB25_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41766.1"
                     /translation="MCTGHRGSIFGTFLDWIEKAEALFGLEIHGDFKLLFLGNSGGGA
                     QAIHRAVSGMTAS"
BASE COUNT         1575 a         2197 c         1953 g         1565 t
ORIGIN      
        1 acgatggtct tgaaaatctc agcgtcccgc aaggtgactt gcccacccgc ttaccaccca
       61 acctaaggaa caagcgatgg aaaccaagaa aatgaaggat gcagccaaca acgcattctt
      121 ggctctcttc agggactaca gcgaagaagg tgtccgcaag tacatcgcag cggacatcat
      181 tcagcacaat ccgtttgtac ccattggacg ggacgccttg attgggttct cgctgacact
      241 caagaagatg ggtatcacct acaccaacca tcgcatcctt cgagacggcg agtttgtcgt
      301 gctgcacaac tcatgtaaca atgcccaagc ctttggtgct ccaaagatcg taaccatcga
      361 catttaccgg atgaaggccg gtcaagccgt agagcactgg gacgccaccg ctccattggc
      421 cgaaaaaacg gccagcggaa ggagccaggt cgacggagca actgagatca cggacatcga
      481 caagactact gcaaacaaag ccctggctgt ttccttgatc gaagatgtct tgatgggcaa
      541 gaaccccagc aagatcaccg agtacatcag cgcagagcag taccaccaac acaaccccgg
      601 catcaaagat ggcttgagcg ggattgtgga agccgaagcg cagatgagcg ctgcagcttg
      661 agctcgacaa gctggcacca cacgatagcg agctcgttgt cgtcatcgcc gccgctggca
      721 aggcgtttcg cgctggccat gatatgcgtg ccagggcatc acacgaccag taccagacct
      781 tgttcaagca atctgccaaa ttgatgttgc gcattcagca gttaccagtg ccggtgattg
      841 ccagggtgca tggcattgcc accgctgcgg gttgccgact ggtggtcatg ctcgaccttg
      901 cggtagcggc cagcagtgcc agtgacagca caagactgcg tgattcgctc tggcgcatcc
      961 aaatcaaatc cactgcactt tattgctcgc ctttgaatgc tcagcttgtt tcattggcgc
     1021 aattttcatt gtcatctgga ggaatctcga acacaaagaa atgggctgat gctgacatta
     1081 aagccgcgtt gaattcagca atgcgatgac agagtgaggc ttgaatgagg tctcaaccag
     1141 cgccggaagg tgaagttggt cacttcgatg gttcaagtgt ggcagcgtgc attgtgggca
     1201 ggtcacagcg acactgttcc atctacacag gtcaccacat caccgccaac ccagacttgg
     1261 cccgctgcgt cttgcgtgat gtgcacgcgg ccctctcggc cgatgcaggt gccttgggcg
     1321 accacataac tgggcggagc gtggccgtcg gcgatcagcc actgcgccag gctggcgtta
     1381 aaactgccgg tgatggggtc ttcgcccaca ccatggcccc ggtcgaagaa gaagcgcact
     1441 tccaggtctg gcgctgccgc tgccgcgttt gtggtgcgtg cgccaaaagc acgtgcctcg
     1501 cggttggagc ggcctatcag tcgcacttgc gctgcagtat cgtggcgagc ggccacgccc
     1561 agcccgctaa aaccgtacgc agccatcagc gcctgcagtt gggccaggtt gggctgaacc
     1621 gccaacaccg attctgcact gtccagcaac acgccaaagt ggtgcgggcc attgatctgg
     1681 tgctgtgcgg cgcgtagctg cgcgggcgca aggcccagcg ccgcaggcaa tgcgttccac
     1741 agcgcgtcgc tcggggcgtt gcgctgcagg ggcggtgctg caaacgccag gcgctggcct
     1801 tgacgcgcca tggccaccag acccaccttg cattgctgca caatgcgcgc cgggtctttg
     1861 ggtacgccgc ctgcttccag ccacgcgtgg caagtgccca gcgtcgggtg gccggcaaac
     1921 ggcatttcgt aggcggtggt gaagatgcga acctggtagt ccgcaccggc tgcggcggcc
     1981 tcgggtgtgg gccgcagcac gaaggtggtt tcactcagat tggtccatgc cgcaaagcgc
     2041 tgcatctcgg catctgacag gccagtgccg tccagcacca cggccagtgg gttgccgagg
     2101 tagggggtgg cggtgaatac gtcaacttgt ttgaacgggc gaatacccga gtgctttagg
     2161 tgcatgaatg tctctttgga tacggcagca gaaatggatc atgacagcgg caagtcgatc
     2221 acccctagaa ccgctggcca ctgcgggatg tggtcaaacc agcgctccaa gtaccgcaac
     2281 tccaagctgc gttggagaaa atcggcattt caatcccaca aggctcagta tcgcaccatg
     2341 ggacggccct cgatttacgg cctttcgtgg ccggttcggc gctgaaaccc ctgtccgata
     2401 aaaggtcaag cttgacctcg cagcgctggg cgacttgcgc ccgggcagtc tctcgactca
     2461 gtgcaacgtt tgcggctccc caggctggta ctgcaagccc gactcgtccg tcaagcacgc
     2521 acgctactgc cagggcaact tcacccgcca aggcaacagc aataccaagt atgtcaagaa
     2581 ggaagatttg ccagaggtgc gcaagcagct caaaaactac cagcgcatga aactgtgatg
     2641 gaggaatggt tcgacttggc cagggcgttg tcaatactgc ggctagccaa gaaaccgcct
     2701 gaatagcggg gaggacttgg cactgtccag cttcatctca tcttgacaac aacttggaca
     2761 cagaccatga ctgaacaagg catcggtgtg ctcggaaata tactgttcca actggcgcca
     2821 tgaatccccg tcatccctta tcttcttgca tcgcatgcag atatgaataa tcccttccaa
     2881 ccgcttcatc cgcgccaggg tggcttccag ctcctgtttc ctgacttcca gcaggtcacg
     2941 ctgctgccgt atgatttcac tgcgtcgatt gagctccaga tggttccgaa ctcgtaactt
     3001 aaccaactcg atgttgaccg gtttcgtcag gtaatcaatg cctcctgctt ccagcccccg
     3061 aatttcgccg tccaaggtgt ccatgccggt gagaaagatg atcgggatgt gggccagttt
     3121 ttcactggac ttgataatgc tgcacacatc aaacccgctc aagtcaggca tcatcacatc
     3181 cagaaggata agttcagggg gatgttcctg gactcggctg atggcctctt ggccaccagt
     3241 ggcagtgtca atgtcatact cgtgccagag ggctgccttc agcagcatga tattggtcac
     3301 atcgtcatca acgatcagta ttctttcttt acctggctcc atgggttatt cctttcgagt
     3361 gaccaccgcc cgccgcacct ctccgccaac gccttgtctc tagcggtttt gacaggcacc
     3421 cgtcgaaatc ctggtccagg tattcccacc gatcctcctt cgagataaaa gcagtcaggg
     3481 caattgccga cacctggcaa cctgttacct tatctttgac acgattgatg cgcaacgcct
     3541 cttctcccaa catctccggt atctgaatat ccatcaacga cagatcacat tggtgcactg
     3601 acagcccgtc atccgctacc gtgcttgctc tgccgtcgta agctgctgtc ccaggacaaa
     3661 tttgttctta tggcatcttc gtcattcccg cgcaggccgg aatccagact gcactagatt
     3721 cccgtctgcg cgggaatgac ggtaaatcaa cgacttggcg aaagaacaaa tttgccctgg
     3781 ctgatgtccc aacctaccta gcaaagccat ggcgcaccgg ttataaatgg cattatcttc
     3841 gacaaggaat atcctcaggg cgagcccatc ccatgcagtc ggcggcaaat ccgattgcgg
     3901 ttgaacgaat tgcgccaggt cgaccacctg aaaggtttgc agacgatgag acatgcgccc
     3961 tgccccttcc cggcttttga catggaatcg tccaccgatc agttccacca gccgacggca
     4021 gatagcgagg ccaagccctg cactgccatg tttacgggca attggaacct cagcttgagc
     4081 gaaaagcatg aaaatttgtt ccaaaatttt cgctgccatg ccaatcccgg tgtcctgttc
     4141 agcaagatga agcaacacgt tgttgccatg gcgttccatc atggttgcag caacgttgat
     4201 agatccatgc accgtgaatt gaatggcgct ccatgagccg aactacaggg gcttccaaaa
     4261 gtgtcaacag gtttttagca ttctcttgat aaactgttcg cgccgattga atgttgaccc
     4321 accctgctgg caaagaaaca gttgccagcc agcggtgcgc agaccccgca gcagcggtcg
     4381 cagcaagtac aacaacagat caaacagcaa gtggaggcgg ccatgcagca ggctccccgc
     4441 gccgttcctg acggcaagta gaccgcactc atggtgcatt gcccggcgct gccactacag
     4501 tttgaacacc gccacggtct gcaccagatc ctgggcctgc gagcgcaggc taccagcggc
     4561 cgccgccatt tgctcgacca gggctgcatt ttgctgggtg ctcatatcca tctgcatcac
     4621 cacctcggca acctgggaga catcggtgct ttgctcatta ttggcggtgc tgatttctcc
     4681 catcaggtcg gtcacgcgct tgatggagtt caccacttcg gtcatcgtga caccggcatg
     4741 gtcgaccagg accgagccgt gctccactcg ctcgacgctg gcggtaatca gggccttgat
     4801 ctcccgcgcc gctgcggcgg agcgtccggc cagcgaccgc acctcgcttg ccaccacggc
     4861 aaagccgcgc ccctgctcgc cggcgcgtgc tgcctccact gcggcattca gcgccaggat
     4921 attggtttga aaagcaatcc cgtcgatcac ctggatgatg tcggcaatct tgcgcgatga
     4981 ggcattgatg ccttgcatgg tttgcaccac ctggcccacc acctcgccac ccttgacagc
     5041 cacgctgctg gcctgcagcg ccaactgatt cgcctggcgt gtgctttcgg cattttgttt
     5101 gacggcctcg ccaagttcgg ccatggaggc atcggtacgc tccagcgccc cggcctgcgc
     5161 ttcggtgcgg ttggacaagt ccatattgcc cgaggcaatt tcagcgctgg ccgaggccac
     5221 actgtcagag ccctggcgca ccttttgcac caccgccgac agattgtttt gcatatggtc
     5281 cagggcgcgc atcaacacgg caatttcgtc attgccggtg gtgtccacct tttgcgtcaa
     5341 atcgccttgc gacacggcct cggcaaaatc caccgcatgg cgcaacggcc gaatgatgct
     5401 gcgcaccagc agcaaaccgg ccgccaccgc caacactacc gccagcgcgc cagctatgct
     5461 ggcaatcatg cgcgtgccag cgatcaggct gctgtttgcg gcccccttgt cctgcacatt
     5521 ggaaacctgc ctcgcattga gcttttccag actggtcata aagggtgcca gcatgggaat
     5581 catctcttcg tcggcggcaa aggctgcttc cgcttgttta cccgccttga ccatgccgac
     5641 gatgcgctgt acgccagcca catagttctt gtggcgttta cgcacctcga ccagaagggc
     5701 actgtcttcc tccgagcccg ccagcgcctt cgccagatcg tccatgtcct tggcaatgtg
     5761 agcatcgcgt tcggctattt ccttggtgag tttggtttgc aggtctgcgc tagaggacac
     5821 caggatcacc atgctggcaa tcgcattgtc cttggccagc aagtgcattt cggtagcttg
     5881 cgcctgcacg cgcacaccgt cgcccatggc ttgcgagttg gcggcactgc ggaacaagcc
     5941 cacctgcact gccaccacca gcagcacgat ggtcagcagc accacactga aggccagagc
     6001 gacgcgcgcg ccaatcttca aatcaaaaag tttcatgcaa tacaacccat tgcctcacgg
     6061 ccctgagtgc gcctgtggat cacggaaatc agccgcccaa ctcgccaacc aattgctggc
     6121 acttggtgta gcgctctttc tggatgccgt tgaccagttt tttggcatcg tcgtccttac
     6181 cggccagaac ggcgggcacc agttcagtct cgcgggtctt cttgaaggca gcccagattt
     6241 cgctcaattc cttgaattgc gcttccttgc cggctggggc tttcatcttg gcgaggtggg
     6301 cgctgacctt gtcggcggtt tccttgacca gcttttgctg gtcggcaccg cgtttgtctt
     6361 tgttgacaac cagggtcacc agggactcgc gcgcagcggt catttcggtg cgcaggtctt
     6421 caaactcgtt ggcaagaacg gcgctagtag cgccaaatcc caacactgca aatgccacga
     6481 tggcaaacca tttcttcata acgacctcct gaatggacat aggcgtgaac aaccatcttt
     6541 tcactgattt actcaataga ctcggcaaag tcatcaaggc gattccccac aagaatctga
     6601 gcagcttccc ttggcacctt tggaatcgtc caccttatag gcaatgacgg gtatgccggc
     6661 cttgaccagc gcgcaaacct cgtcacggta ggcctggtcc tcaatgatgg tgagcgcaac
     6721 accatcaggc ttggccgaca aggccttccc accagcgact tgtagcgatc cagactacca
     6781 ccggcagact ggaagtcgac atgtgcaccg gccatcgcgg cagcatcttc ggcacctttc
     6841 ttgactggat tgaaaaagcc gaagccctct tcggtctgga aattcatggt gatttcaagc
     6901 ttctttttct gggcaacagc gggggggggg cgcaggcaat ccacagggct gtaagcggta
     6961 tgactgcaag ttgactgcga gttgactgga ttgactgacc agtgacatgg ttaactcctg
     7021 aatgctgcac gaacaagcga cccgaaccgg gatcgccagg gaaattgatc tgtttcccca
     7081 cagcagcact ggctacttta cagaagcgcg caatagatgt aaatcatcca aatctgaatt
     7141 tgctctcctc aaatcttagt tatttatgag aaatgtctga cactggcgac aactgctttc
     7201 gagcgatttc tatcgaggct tggctaacat gcagggttga tgcacacttg cctgcccgtt
     7261 gagtccattt ccccgaccgc tcaaaaccca
//
LOCUS       DAOSLL010000170         7267 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9373_length_7267_cov_4.247643, whole genome shotgun sequence.
ACCESSION   DAOSLL010000170 DAOSLL010000000
VERSION     DAOSLL010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9373_length_7267_cov_4.247643"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1203)
                     /locus_tag="PLB25_09090"
     CDS             complement(<1..1203)
                     /locus_tag="PLB25_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HOE41767.1"
                     /translation="MKTMAHIAAELAGYDPQAMSADAVNAFLARLVVPVKQIEIVDIF
                     NALGRVLAVDIVSPINVPPHDNSAMDGYAFDGAQLSPGQPLLLKVIGTALAGQAWRGP
                     VAAGECVKIMTGAVMPAGLNTVVPQELVSHNAVADPAATGNLATIRIAPGLLQPGDNR
                     RFKGEDLQQGRSALRKGELLTPAAIGLVASLGMKTVPVLARLKVAYFSTGDEILSLGE
                     APREGTVYDSNRYTIFGLLTRLGCEVIDFGAVPDKPVLLEAAFLQAAAQADVIITSGG
                     VSAGEADHTRLMMKKLGDVAFWRVAMRPGRPMAVGQIFQDKLASRPSGTSANSYVTNS
                     VSSAPAILFGLPGNPVAVMVTFLAFVRPALLRMMGCTKAAPPLLKARSSEPIRKNPGR
                     TEYQRGIVS"
     gene            complement(1200..1835)
                     /gene="mobA"
                     /locus_tag="PLB25_09095"
     CDS             complement(1200..1835)
                     /gene="mobA"
                     /locus_tag="PLB25_09095"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HOE41768.1"
                     /translation="MIAAKDITALILAGGRGSRMGGLDKGLQTFNGMPLALHTLTRLQ
                     SGSGVGSIMINANRNLSAYKSFGVPVWPDGLADYAGPLAGFLTGLAHCETPYLLTVPC
                     DTPLLPLDLASRLAAALDNANAELAMAAAPEPGRNSQLLLRVQPVFCLLRAALRTSLA
                     QFTQLGGRKVDAWTARHHTAVAAFDQPGDHPQAFFNANTLAQLRQIEGCTP"
     gene            complement(1837..2997)
                     /gene="moaA"
                     /locus_tag="PLB25_09100"
     CDS             complement(1837..2997)
                     /gene="moaA"
                     /locus_tag="PLB25_09100"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HOE41769.1"
                     /translation="MTERDTHRVIPLQDQRNAALAAPAAAFAPDSRLTADGLLADQRG
                     RPLRDLRISVTDRCNFRCNYCMPKAIFDKDYAYLPHSALLTFEEITRLSSHFVAHGVQ
                     KIRLTGGEPLLRKSLERLVEQLAALRSPQGVPLDLTLTTNGALLARKAKALKAAGLQR
                     VTVSLDSLDDGLFRSMNDVDFPVADVLAGIDAAQAAGLGPIKINMVVKRGTNEQQILP
                     MARHFRGSGMVLRFIEYMDVGATNGWRMNQVLPSAEVVQRIRAELSLVPLAPGQPGET
                     AQRWGYADASGQHDRAAGEIGVISSVTQAFCGDCNRARLSTEGQLYLCLFAGQGYDLR
                     SLLRGNAPDADLASAIAHIWQGRTDRYSELRSALGPDTSAAVKRVEMSFIGG"
     gene            complement(3317..5383)
                     /locus_tag="PLB25_09105"
     CDS             complement(3317..5383)
                     /locus_tag="PLB25_09105"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HOE41770.1"
                     /translation="MTSNSAALILALICGLIAVGYGFWARSWILAQDAGNPRMQEIAA
                     AIQTGAAAYLARQYKSIAMVGVVLTILIAVFLDSLSAIGFVVGAVLSGACGFIGMNVS
                     VRANVRTAQAATKGIGPALDVAFRGGAITGMLVVGLGLLGVTGFYWFLVPDGATTAAK
                     LNPLIGFAFGSSLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNV
                     GDNVGDCAGMAADLFETYAVTLIATMVLGALMVTGAGTNAVVYPLALGAVSIVASIIG
                     CFFVKASPGMTNVMPALYKGLAVAGVLSLVAFYFVTQNLFPTPLTLADGHVISAGSLF
                     ASCAVGLILTAALVWITEYYTGTQYKPVQHIAEASTTGHGTNIIAGLGVSMRSTAWPV
                     MFVCLAIYTAYAMAGLYGIAIAATSMLSMAGIVVALDAYGPITDNAGGIAEMSEMPKS
                     VRDITDPLDAVGNTTKAVTKGYAIGSAGLAALVLFADYTHKLEGLGAHITFDLSNPKV
                     IIGLFIGGMIPYLFGAMAMEAVGRAAGSVVVEVRRQFREIKGIMDGSGKPEYDTAVDM
                     LTTAAIKEMMIPSLLPVVVPILVGLILGPEALGGLLMGTIVTGLFVAISMCTGGGAWD
                     NAKKYIEDDNFGGKGSEAHKAAVTGDTVGDPYKDTAGPAINPLIKIINIVALLIVPVM
                     MKLHAG"
     gene            5616..6152
                     /gene="ppa"
                     /locus_tag="PLB25_09110"
     CDS             5616..6152
                     /gene="ppa"
                     /locus_tag="PLB25_09110"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HOE41771.1"
                     /translation="MSLDNVTPGANAPEMFNVIIEIPMNADPVKYEVDHDTHAIFVDR
                     FMSTAMHYPTNYGYVPHTISGDGDPVDVLVVTPMPLIPGVVVTCRAIGILKMEDEAGI
                     DGKVLAVPIPKILSLYSRWNKPEDLSPTRLKTIAHFFEHYKDLEDGKWVKILGWEGPE
                     AAKQEIMEGIANYQKSLV"
     gene            6269..7123
                     /locus_tag="PLB25_09115"
     CDS             6269..7123
                     /locus_tag="PLB25_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015603559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOE41772.1"
                     /translation="MKLLRYGPLGQEKPGVLDELGRVRDLSGVVADVAGEVLLPQSLA
                     RLRALDLASLPLVAGLAQQDLRLGACVSQVGKIICIGLNYADHAAESGVSVPPEPVVF
                     GKWTSALMGPDDAVQIPRGSLKTDWEVELAVVIGQGGRYIAQADAMSHVAGYCVMNDV
                     SERDYQLNRSGTWDKGKGCDTFAPLGPWLVTADEVPDPQALALWLEVDGKRYQNGSTG
                     TMVYQVPFLISYLSQFMSLQTGDVISTGTPPGVGMGQKPPVFLRAGQTMRLGVQGLGV
                     QTQRVVQG"
BASE COUNT         1463 a         2135 c         2264 g         1405 t
ORIGIN      
        1 gctgacaatg ccacgctggt actctgtgcg accgggattc ttgcgaatgg gttcgctgct
       61 gcgggctttg agcaggggcg gcgcggcttt ggtgcagccc atcatgcgca acagggcagg
      121 gcggacaaag gcgagaaagg tcaccatcac cgctaccggg ttgcccggca gcccaaacaa
      181 aattgccgga gcacttgaaa cgctattggt aacatagctg ttagcgcttg ttccagaagg
      241 tctggaggcc aatttgtcct gaaaaatctg acccacggcc atgggtcggc cagggcgcat
      301 ggcgacacgc cagaaagcca cgtcaccgag ctttttcatc atcaagcggg tgtggtcggc
      361 ctcaccagcg ctgacaccgc cgctggtgat gatcacgtca gcttgcgcgg cggcttgcaa
      421 gaacgcggct tcaagcagca ctggtttgtc cggcaccgcg ccgaagtcga tcacttcaca
      481 gcccagccgg gtcagcagac cgaagatggt gtagcggttg ctgtcataca ccgtcccttc
      541 acgcggggcc tcgcccaggc tcaggatttc gtcgccggtt gaaaaatagg caaccttcag
      601 gcgcgccagc accggcacag ttttcatacc caaactggcc accagcccaa tagcagcggg
      661 cgttagcagc tcaccctttc gtagcgctga gcgaccttgc tgcaggtctt cgcccttaaa
      721 acggcggttg tcaccaggct gcagcaggcc cggcgcaatg cggatggtcg ccaaattgcc
      781 ggttgcagcg ggatcagcca cggcgttgtg gctgaccagt tcctgtggca ccacggtgtt
      841 gagcccggct ggcatgacgg cgccggtcat gatcttcacg cattcgccag ccgccaccgg
      901 cccgcgccag gcctgtcccg ccagcgcggt gccaatcacc ttgagcaaca gaggctggcc
      961 ggggctaagt tgggcgccat cgaaggcgta gccgtccatc gccgaattgt catgcggtgg
     1021 cacgttgatg ggcgagacga tgtcaaccgc cagcacacgg cccagtgcgt tgaagatgtc
     1081 aacgatttca atctgtttga cgggcactac cagcctggcc aaaaaggcat tgaccgcgtc
     1141 tgcgctcatc gcctgcgggt catagccagc cagctcggca gcaatgtgcg ccattgtttt
     1201 catggtgtgc aaccctcgat ttgccgtagt tgagccaggg tgttggcgtt gaaaaaggcc
     1261 tgcgggtggt cgcccggctg gtcaaaagcc gccacagcgg tatggtgacg ggcggtccag
     1321 gcatcgacct tgcgtccgcc aagctgggta aactgcgcca ggcttgtccg caaggccgcg
     1381 cgcagcaggc aaaacaccgg ttgaacgcgc aacagcagtt gactgttgcg gccaggctca
     1441 ggtgctgcag ccatggccag ttcggcattg gcgttgtcca gcgccgctgc cagtcggctg
     1501 gccaaatcca gtggcagcag cggggtgtcg cacggcacag tcagcaaata cggggtctcg
     1561 cagtgcgcca gcccggtcaa aaagccagcc agtggcccgg cgtaatcggc caggccatcg
     1621 ggccagaccg gcacgccaaa ggacttataa gccgacagat tccggttggc gttgatcatg
     1681 atgctgccaa ccccggagcc gctttgcagc cgggtcaggg tgtgcagcgc cagcggcatg
     1741 ccgttgaagg tttgcagacc cttgtccagc ccgcccatgc gcgagccgcg gccacctgcc
     1801 agaatcagcg cggtgatgtc tttggcagcg atcaattcat cccccgatga aactcatttc
     1861 aacccgtttg accgctgcgc tggtgtctgg ccccaaggcg ctgcgcagct cagagtaacg
     1921 gtcagtgcgg ccctgccaga tgtgagcgat ggcgctggcc aggtcggcgt ccggggcatt
     1981 gccgcgcaac aggctgcgca ggtcgtaacc ctgcccggca aacaggcaca aataaagctg
     2041 cccctcggtg ctgaggcggg cgcggttgca gtcaccacaa aaggcttgcg tgacgctgct
     2101 gatgacgccg atctcgccgg ctgcccggtc gtgctgaccg ctggcatcgg catagcccca
     2161 gcgctgggcg gtttcaccgg gctggcctgg tgcaagcggt accagtgaca gttcggcgcg
     2221 gatgcgttgc accacctcgg cagagggcag cacctggttc atgcgccagc cattggtggc
     2281 accgacatcc atgtactcta tgaagcgcag caccatgccg ctgcccctga aatgccgcgc
     2341 catcggcaag atttgctgct cgttggtgcc gcgcttgacc accatgttga tcttgatcgg
     2401 gcccagaccc gctgcctgcg cggcatcgat acccgccagc acgtcggcca ccggaaagtc
     2461 gacatcgttc atgctgcgaa acagcccgtc gtcgagactg tcgaggctga ccgtaacccg
     2521 ctgcagcccg gcagctttga gcgctttggc cttgcgtgcc agcagtgcac cgttggtggt
     2581 tagcgtcaaa tccagtggca caccttgtgg cgaacgcagg gcggcgagct gctcgaccag
     2641 tctttccagg ctcttgcgta gcagcggctc accaccggtc aagcggattt tctgcacgcc
     2701 gtgggcaaca aaatggctcg acaggcgggt gatttcctca aacgtcagca gcgccgagtg
     2761 cggcaggtag gcgtagtcct tgtcaaaaat cgccttgggc atgcagtagt tgcaacgaaa
     2821 gttgcaacgg tcggtgacgc tgatgcgcag gtcgcgcaag ggtcggccgc gctggtctgc
     2881 cagcagcccg tcggccgtca gtcttgagtc tggcgcaaat gcggccgccg gtgctgccag
     2941 tgcagcattg cgctggtctt gcagcggtat cacacggtgc gtgtctcgtt cggtcatgcg
     3001 cggtgctatg ggttctgggg gggtgaatgg aagaacgtca tgcttcgccg tgattctggc
     3061 acgtttggcg gacctcaaga gagcttgtca gttcattcgg gcaagcccga gccacggtca
     3121 gggaagccag atcaactcga aacaatcaag gtgcgccgca gcggccctgg cgcgtcatgg
     3181 cgaccttgcc attgggtggc aaaccctgcc gggagtaccg tcggggatag acaatcaacc
     3241 ccgcgccctg gtcaggcaac tgccacaaaa aaagcccgct gcagcgggct tttgacgcgg
     3301 tagatcgggt caatgatcag ccggcatgca gtttcatcat gaccggcacg atcaacagtg
     3361 cgacgatgtt gatgatcttg atcagcgggt tgatggccgg accggcggtg tccttgtagg
     3421 ggtcgccaac ggtgtcacct gtcactgcgg ccttgtgtgc ttcagaaccc ttgccgccaa
     3481 agttgtcgtc ttcgatgtac tttttggcgt tgtcccaggc accgcctccg gtgcacatgg
     3541 aaatggcgac aaacaggcca gtcacgatgg tgcccatcag cagaccacca agtgcctccg
     3601 gaccaagtat caggccgacc aaaattggca ccaccacagg cagcagcgag ggaatcatca
     3661 tttctttgat agcggcggtg gtcagcatgt ccactgcggt gtcgtactca ggcttgccgg
     3721 agccgtccat gatgcccttg atttcgcgga actggcggcg cacttcaacc accacgctgc
     3781 ccgccgcgcg gcccaccgct tccatcgcca tggcgccaaa caggtaagga atcatgccgc
     3841 caatgaacag gccaatgatg accttggggt tgctcaaatc aaaggtaatg tgcgcgccca
     3901 ggccctcgag cttgtgggtg taatcggcaa acagcaccag ggcggctaac ccggctgagc
     3961 caattgcata gcctttggtg acggctttgg tggtgttgcc caccgcgtcc agcgggtcgg
     4021 tgatgtcacg cacgctcttg ggcatttccg acatttcggc aatgccgcca gcgttatcgg
     4081 tgatcgggcc ataagcgtcc agcgccacca caataccggc catgctgagc atcgaagttg
     4141 ccgcaattgc aatgccgtag agaccggcca tggcataggc ggtgtagatg gccaggcaga
     4201 caaacatcac tggccaggcg gtagagcgca tcgacacccc caggccggcg atgatgttgg
     4261 tgccgtggcc ggtggtcgag gcttcggcga tgtgctgcac cggtttgtac tgggtgccgg
     4321 tgtagtactc ggtgatccag accagggcag ccgtcaatat gaggccaacc gcgcaggatg
     4381 caaacaggct acccgcgctg atgacatggc cgtcggccag ggtcaggggt gtcgggaaca
     4441 aattctgcgt gacaaagtaa aaagccacca gcgacaacac gcccgccacc gccaggccct
     4501 tgtacagggc tggcatcaca ttggtcatgc ccggactggc cttgacgaaa aagcagccaa
     4561 tgatggaggc cacaatcgac accgcaccca aagccagcgg ataaaccacc gcattggtgc
     4621 cagcgccggt gaccatcagc gcaccgagca ccatggtggc aatcagcgtc acggcgtagg
     4681 tttcaaacaa gtcagcggcc atgccggcgc aatcacccac gttgtcaccg acgttgtcgg
     4741 caatcaccgc cgggttgcgc gggtcgtctt cgggaatacc ggcctccacc ttgccgacca
     4801 ggtcagcgcc gacatcggca cccttggtga aaataccgcc gccaagacgg gcaaagatcg
     4861 agatcagcga tgagccaaag gcgaaaccaa tcagcgggtt cagtttggcc gctgtggttg
     4921 caccgtctgg caccaaaaac cagtaaaagc cggtgacacc caataaccca agccccacca
     4981 ccagcatgcc ggtgatggca ccgccacgaa aggccacgtc aagggccggt ccaatgcctt
     5041 tggtggcggc ctgggcagtg cgcacgttgg cgcgcaccga gacattcatg ccgataaagc
     5101 cacaagcacc cgacagaacg gcacccacca caaagccaat cgcactcaaa ctgtccagaa
     5161 agacggcaat caaaatggtc agcactacgc cgaccatggc gatgcttttg tactgacgcg
     5221 ccaggtaggc ggcagcaccg gtttgaatgg cggcggcgat ttcctgcatg cgcgggttgc
     5281 cagcgtcttg ggcgagaatc caacttcggg cccaaaaacc gtagcctacg gcaatcaagc
     5341 cgcagatgag cgcgagtatt agcgctgctg agttgcttgt catgtgatga agctcctgaa
     5401 gtgttgtttc gccgggttgt ggtgcaaaac aattggcacc accgctgcgt tgcttcctcg
     5461 tcaaaggccg cctgggtgac ggacggcggt ggtgattggc caacggttgc actttaacgc
     5521 taaaacagcg gttttttccg ttgcgcaagt ggtgagtaag caaaatcagg gttaatcctc
     5581 ggtgttgccg aattcactca ctaccaagtt tcaccatgtc tttagataac gtcacccccg
     5641 gtgccaatgc ccctgaaatg ttcaacgtga tcatcgaaat tccgatgaac gccgatccgg
     5701 tgaaatacga ggttgaccac gatacccatg ccatttttgt tgaccgtttc atgagcacgg
     5761 ccatgcatta cccgaccaat tatggttatg taccccacac catcagtggt gacggcgacc
     5821 cggtcgatgt gctggtggtg acacccatgc ccttgattcc cggtgtggtg gtcacgtgcc
     5881 gcgccatcgg cattttgaag atggaagacg aagccggtat cgatggcaaa gtgctggcgg
     5941 tgccaattcc aaagattttg tcgctctatt cgcgctggaa caagccggaa gacctgagcc
     6001 cgacccgcct caagaccatt gcccactttt ttgagcacta caaagacctc gaagacggca
     6061 agtgggtcaa gattctgggc tgggaagggc ctgaggccgc caaacaggag atcatggaag
     6121 gcatcgccaa ctaccagaag tccttggtgt gattttttgc cagtagccct tatggaataa
     6181 gcgctgccag ctatatattt tgaagcgccc gctggcttgt gccagtgggc gttattgttt
     6241 tggcttggtg ccaattgagg agtggtggat gaaattactg cgttatgggc ccttgggcca
     6301 ggaaaagcct ggggtgctcg atgagttggg gcgggtgcgg gacttgagtg gggtggtggc
     6361 tgatgtggct ggcgaggtat tgttgccgca gagtctggcg cgcttgcgcg cgctcgacct
     6421 ggcatctttg ccgctggtgg ctggtctggc gcagcaggat ttgcgcttgg gcgcctgcgt
     6481 cagccaggtc ggcaaaatca tctgcatcgg actgaactat gccgaccatg cggctgagtc
     6541 gggcgtgtcg gtacctcccg agccggtggt gtttggcaaa tggaccagtg cgctgatggg
     6601 gcccgatgat gccgtccaga ttccgcgcgg ttcactcaag accgactggg aagtggaact
     6661 ggcagtggtg atcgggcagg gcgggcgcta catcgcgcag gccgacgcga tgagccatgt
     6721 ggcgggctat tgtgtgatga acgatgtttc cgagcgcgac taccagctca accgcagcgg
     6781 tacctgggac aagggcaagg gctgcgatac gttcgcgccg cttggtccct ggctggtgac
     6841 ggccgatgag gtgcctgatc cgcaggcgct tgcgctctgg cttgaagtcg atggcaagcg
     6901 ttatcagaac ggcagcaccg gcaccatggt gtatcaggtg ccgtttttga tcagttacct
     6961 gagccagttc atgagtttgc aaaccgggga cgtgatttca accggcacgc cgcccggcgt
     7021 tggcatgggc caaaagccgc cagttttttt gcgcgccggg caaaccatgc gcctgggcgt
     7081 gcaagggctt ggcgtgcaga cgcagcgggt cgtgcagggg taagtagcag tccgttgttg
     7141 atggggcgat gtgctggcag ctcaggcgag gcacatgcgc tcagacagag ggcctcatac
     7201 ccgcttcgct ttgcgaaatc ggccccctgc ctgagcgaac atcacctgag aagttgatgg
     7261 gggtcat
//
LOCUS       DAOSLL010000171         7256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9385_length_7256_cov_5.286210, whole genome shotgun sequence.
ACCESSION   DAOSLL010000171 DAOSLL010000000
VERSION     DAOSLL010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7256
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9385_length_7256_cov_5.286210"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..753
                     /locus_tag="PLB25_09120"
     CDS             <1..753
                     /locus_tag="PLB25_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE41773.1"
                     /translation="NIDPRVDATLRAWDKDGAYSLPYMWYTVGIAWNPRLTAKAFPGH
                     VMDSLTSVFDPDIARRFQSCGVGVVDSASDVVPLAAMAGGVAKWDGKNSGAAAARVLD
                     RLSGVVKVIPTDQFVDSLASGKVCVAIGFSGDAVQAQAKSRGTVEYRVPADGGLLAID
                     AMAVPANAKNKREAHLFIDYILRPQVIAKISNTVGYANANLKAGEFMSDAVKSNPAVV
                     PTPAALSRSSPIPILSEQDQQDIARIWAKFAK"
     gene            788..1621
                     /locus_tag="PLB25_09125"
     CDS             788..1621
                     /locus_tag="PLB25_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41774.1"
                     /translation="MKHPMNRVALLAAGVVGIFASIPLGWSGLLLVAVMALGCEVLAA
                     LMIPSLPSFRASVDREQRMQSRALRARQLISEINQRGDGQALATYWQMLERVQALRQT
                     VQEHRTSLSLEDVDKLEDMTLDYLGLCAVNLSLKQRKDHASEDVAAKRIASLQAQLAR
                     QNLPEDEARQLRAALAEYTETMQRSRRLAVRRSALEATLISLPDKMEEVYQLVITSPY
                     STEMGSKLEESLSRLRIAEEVAAEFDTPDSFDLEPSRTASAASRAPLQAARPAAGSVK
                     T"
     gene            1667..2380
                     /locus_tag="PLB25_09130"
     CDS             1667..2380
                     /locus_tag="PLB25_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HOE41775.1"
                     /translation="MANNAIFARLSNLWTGFISLWVSDIEKEHPDIAYQNAIASMIEK
                     YTQLKAATGAIIARRQDITARLQSHERDLASVAADLETALATSQDDLAVVLIQKKNAL
                     DVAITELRADATQATADADNAKDSLTQVKTEIDKLKAEKDRMLAQMHSAQARLKIQGQ
                     LDGLSVDAEVQALGAVRDHIKGQVAQASLGSELRESDLDVRLNKLRQSSGSVTAKAQL
                     EAMKQARAAAAQSAPAKTL"
     gene            2394..4229
                     /locus_tag="PLB25_09135"
     CDS             2394..4229
                     /locus_tag="PLB25_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE41776.1"
                     /translation="MAATAPPRADLPRWAETLRQKYLAGEASTFVLYRNVFDNFLVSG
                     KLHGLQSFLVEELFKDTKKRVVEVSLERGIRHLSGKPNVALAALSQDSGLEPSLLQSL
                     HVLEAQMRSEQSTAVIVPYADALLPAGEPSFMAQQDRQTYLAFHRWSLDASLTQGDNI
                     TILITESLGAINPGLLSNPKVSAIEIPMPDLAQRKRVIAFFAPKLNEQQVTLFSERTG
                     GLRTVQLASIMASSDGHGLSEGQRRELILQLLQGTPEAEQRASTLAAITAGMTPSEIN
                     KLIEPGKSLPVGDANAEVLKVIHTRKREMIEKECAGLIEFIEPKHGLASVGGHENIKA
                     ELLAIAKNLKEGNTTLTPMGLLAVGPMGSGKTFVIKAFLKEAGLSGVALKNFRSKWVG
                     STEANLERVLATVKAMGPIAVIIDEGDRSFGNGGGEGGDDGTSSRIIARLKEFMAETE
                     NRGQVLFVMMTNRPDKLDTDIKRPGRLDRKIPFFYCETALERSQVLLAVLSRYAETCV
                     ASTAELLTLCETLTGYSNADLEALALLAVESARNQARALDAEVLQQAAQDFMPPQERD
                     MIEFMELLAVSETSRRTMLPKRFRDMAMADIQSNLQSARRRALAR"
     gene            4370..4828
                     /locus_tag="PLB25_09140"
     CDS             4370..4828
                     /locus_tag="PLB25_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HOE41777.1"
                     /translation="MPPVQDQNLSSAQVIALTRAMLSVAAVEGIQPAEAALIGQFYEA
                     SRQPDMPTTAALLGNPDPGQFELGELAGCPAALADTVVLMCLMTAYADGHLSDAERAK
                     VQAIASELKLSDARFEALLAQVRDELVGALSHLPDAGSVAVVVKELAAAD"
     gene            5022..>7256
                     /locus_tag="PLB25_09145"
     CDS             5022..>7256
                     /locus_tag="PLB25_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HOE41778.1"
                     /translation="MRRVKAGFTLFLIAVSAHSVWAGATFDEVKASWQPSDTRILDRH
                     GEELHRLRTDASVRRGQWVALADVSPALRTAMVLSEDKRFFEHSGVDWQAVSAAAWAN
                     LWNTRTRGASTITMQLAGLLDDDLKRSAGGRSVLQKVSQTVSAQWLERSWRKDQILEA
                     YLNLVSFRGELVGIDALSQTLFGKAAHGLDAREAAVAAALVRAPNAKPAQVAQRACAV
                     LASMALANAAKPAAAKPGSVASASATSAGASAAKPGAAKPGSVAPASTASAGANAASS
                     DAAGRCDALAFFTTAALQRRDFAPSAGLAPHLARQVLATQATRPDRRTNSAAASKPTA
                     GARAATEIRTTLLAPLQRFAVQTLQQHLRELDGRHVEDAALLVLDNASGEVLAWVGSS
                     GELSRAGDVDAVTALRQPGSTLKPFLYAQAIAEGRLTAASLLEDSSTQIQTSGGLYIP
                     QNYDRQFKGWVSVRTALGASLNVPAVRTLVMVSPDAFHQQLVKLGLPLPESGGYYGYS
                     LALGSAEVSLLNLTNAYRSLANGGLHGPISLSWPDDRSAPPAQATSVATPSARQKPAT
                     LRQVIDARAAFIVSDILSDPLARARTFGMDSVLATRFWTAVKTGTSKDMRDNWALGYS
                     QRYTVGVWVGNASGAPMWDVSGTTGAAPIWAALMNYLHRQTPSHPPRPPPGVIRTAVQ
                     FAVSGASANRPVMPEAARQEWFVQGTQQTTFAINLEASSAYASTAGDQKGLNSPAQQA
                     VNAGT"
BASE COUNT         1443 a         2314 c         2205 g         1294 t
ORIGIN      
        1 aacatcgacc cgcgcgtcga tgccaccctg cgcgcctggg acaaggacgg tgcctacagc
       61 ctgccctaca tgtggtacac ggtcggcatt gcctggaacc ccaggttgac cgccaaagcc
      121 ttccccggac acgtcatgga ttcactcacc agcgtgtttg accccgacat cgcccggcgc
      181 tttcagtcct gcggggtggg cgtggtggac tcggccagcg acgtggtgcc gctggcagcc
      241 atggccggtg gcgtcgccaa gtgggatggc aaaaattccg gtgccgcagc ggccagggtg
      301 cttgatcgcc tgagcggtgt ggtcaaggtc atcccaactg accagtttgt tgattcgctg
      361 gccagcggca aggtctgcgt ggcgattggc ttttcgggcg atgcggtgca ggcccaggcc
      421 aaatcgcgcg gcacggtgga ataccgggta ccggccgatg gtggcttgct ggccattgat
      481 gccatggcag tgcccgccaa cgcgaagaac aagcgtgaag cgcatctgtt cattgactac
      541 atcctgcgcc cgcaggtgat cgccaaaatc tccaacacag ttggttatgc caatgccaac
      601 ctgaaggccg gtgagttcat gagtgatgcc gtcaaatcca acccggcggt ggtgccaacc
      661 ccggcggcgc tcagccgctc gtcaccgatt ccgatcctgt ccgagcagga ccagcaagac
      721 atcgcccgca tctgggccaa gtttgccaaa taacgtggca ccaaagccgt cttatctggg
      781 tgccttcttg aagcacccaa tgaaccgggt ggccctgctg gcagccgggg tggttggcat
      841 ttttgcctcg atcccgctgg gctggtcggg tttgctcctg gttgcggtga tggcactggg
      901 ctgcgaggtg ctcgcagcgc tgatgattcc cagcctgcct tcgtttcggg cctcggttga
      961 ccgcgaacaa cggatgcagt cacgtgcgct gcgcgccagg caactgatca gcgaaatcaa
     1021 ccagcgtggc gacggccagg cgctcgccac ctactggcag atgctggagc gggtccaggc
     1081 gctaaggcag accgtgcagg aacatcgcac ctcgctgagc ctggaagatg ttgacaaact
     1141 tgaagacatg acactcgact acctcggcct gtgtgccgtc aacctgtcac tcaaacaacg
     1201 caaggaccac gccagcgaag atgtggcagc caaacgcatc gcctccttgc aggcccaact
     1261 ggcacgacaa aacctgccgg aagacgaggc ccgccagttg cgcgccgcgc tggccgaata
     1321 caccgaaacc atgcagcgct cgcgtcgcct ggcggtgcgc cgcagcgcgc tggaggccac
     1381 gctgatctcg ctgcctgaca agatggaaga ggtctatcag ctcgtcatca cctcgcccta
     1441 ctcgaccgag atgggcagca agctcgaaga atcgctgtcg cgcctgcgca ttgccgaaga
     1501 agttgccgcc gaattcgaca cccccgacag ttttgacctt gagccatcca gaacggccag
     1561 cgctgcaagc agggcaccgc ttcaagccgc ccgcccggct gctggcagcg tcaaaacctg
     1621 acgatctttt tttaaacccc agccctcgtc acccaggaga acccccatgg ccaacaacgc
     1681 aatcttcgcc cgtctgtcca acctctggac cggcttcatt tccctttggg tctcggacat
     1741 cgaaaaagag catccggaca tcgcctacca gaacgcgatt gcctcgatga ttgaaaaata
     1801 cacccagctc aaagccgcca ccggtgccat catcgcccgc cgccaagaca tcacggcccg
     1861 gctgcaatcg catgagcgcg atctggccag tgttgcggcc gacctggaaa cggccctggc
     1921 caccagccag gacgacctgg cggtggtgct gatccagaag aaaaacgcgc ttgatgtggc
     1981 catcaccgag ctgcgtgccg atgccacgca ggccacggca gatgccgaca acgccaaaga
     2041 ctcgctgacc caggtcaaaa ctgaaattga caaactcaag gccgaaaaag accgcatgct
     2101 ggcacaaatg cacagcgccc aggcccgcct gaaaattcag ggccagctcg atggcctgtc
     2161 ggtcgatgcc gaagtgcagg cgctcggtgc ggtgcgcgac cacatcaagg gccaggtggc
     2221 acaagccagc ctggggtcgg aactgcgcga gtctgacctg gacgtgcggc tgaacaaact
     2281 gcgccaaagc agtggcagtg tcaccgccaa ggcgcaactc gaagccatga aacaggcccg
     2341 tgccgccgcc gcccagtcgg caccggccaa aaccctctag ctgcggttca accatggctg
     2401 caacggcacc cccacgcgcc gacctgcccc gctgggccga aaccctgcgg cagaaatacc
     2461 tcgccggtga agcctccacc tttgtgctgt accgcaacgt gttcgacaac tttctggtct
     2521 caggcaagct gcatggcctg cagtcgtttc tggtcgagga acttttcaag gacaccaaga
     2581 agcgggtggt cgaggtcagc ctggagcgcg gcatccggca cctgagcggc aagcccaatg
     2641 tggcgctggc agccctgtcg caagacagcg ggctcgagcc cagcttgctg caatcgctgc
     2701 acgtgcttga agcccagatg cgcagcgaac aaagcaccgc tgtcatcgtg ccttatgccg
     2761 atgcgctgtt gcccgccggt gaaccgagct tcatggcgca gcaagaccgc cagacctacc
     2821 tggcgtttca ccgctggtcg cttgatgcca gcctgaccca gggcgacaac atcaccatct
     2881 tgatcaccga gtcactcggt gccatcaacc ccggcctgtt gtccaacccc aaggtgtcgg
     2941 cgatcgaaat cccgatgccc gacctggcgc agcgcaagcg ggtgattgca ttttttgccc
     3001 ccaagttgaa cgaacagcag gtcacgctgt tttccgagcg caccggcggc ttgcgcacgg
     3061 tgcagttggc cagcatcatg gccagttcgg acggccatgg cctgagcgag ggccagcgtc
     3121 gcgagctgat tttgcaattg ctgcaaggca caccagaggc cgagcagcgc gccagcacgc
     3181 tggcagccat cacggccggc atgaccccgt ctgaaatcaa caagttgatc gaacccggca
     3241 agtcgctgcc ggtgggcgat gccaatgccg aggtgttgaa agtcatccac acccgcaagc
     3301 gcgagatgat cgagaaagaa tgcgccggac tgatcgagtt catcgaaccc aaacatggcc
     3361 tggccagcgt cggtggccac gaaaacatca aggcagagtt gctggcgatt gccaagaacc
     3421 tgaaagaagg caataccacc ctgaccccga tgggtttgtt ggcggtaggg ccaatgggct
     3481 cgggcaaaac ctttgtcatc aaggcgtttt taaaggaagc cgggctctca ggtgtggcgc
     3541 tgaaaaactt ccggtccaaa tgggttggct cgaccgaggc gaacctggag cgggtgctgg
     3601 ccaccgtcaa ggcgatgggg ccgattgcgg tgatcattga cgaaggcgac cgcagctttg
     3661 gcaatggcgg tggcgaaggt ggcgacgacg gcaccagctc gcgcatcatt gcgcgcctga
     3721 aagagttcat ggccgagacc gaaaaccgtg gtcaggtgct gtttgtcatg atgaccaatc
     3781 gtcccgacaa gctcgacacc gacatcaaac gccccggccg actggaccgc aaaattccgt
     3841 ttttttattg cgaaactgcc ctggagcgct cacaggtatt gcttgccgtg ctatcaagat
     3901 atgcagaaac ttgcgtcgcc agcacggccg aattgctgac cctgtgcgaa acgctgacgg
     3961 gttattcgaa cgccgacctg gaagcgctgg cgctgctggc ggtcgagtcg gcgcgcaacc
     4021 aggccagggc gcttgatgcc gaagtgctgc agcaggccgc acaagatttc atgccgccac
     4081 aagagcgcga catgatcgag ttcatggaac tgctggcggt gtccgagacg tcccggcgca
     4141 ccatgctgcc caaacgcttt cgcgacatgg ccatggccga catccagagc aacctgcaaa
     4201 gtgcccgcag gcgtgcgctg gcccgttaac cttcatggca aagctgcgcg cgcatgatgc
     4261 aagtctgtag ccacggctgg cagcggcagt tctggcgacc agatgccgtt tgaaccgatt
     4321 gtttcatcat cacatttttc aaacctttcc ccccaatcag gagaccacca tgccccccgt
     4381 ccaggatcag aacttgagca gcgcccaagt cattgcactc accagagcca tgttgtcagt
     4441 agccgccgtt gaaggcattc aacccgccga agcggccttg atcggccagt tttacgaggc
     4501 ctcgcgacaa cccgacatgc ccaccacggc agcgctgctg ggcaaccccg acccgggtca
     4561 gtttgagttg ggcgagctgg ctggctgccc ggcagcgctg gctgataccg tggtgctgat
     4621 gtgcctgatg acggcttatg ccgatggtca cctgagtgac gccgagcgcg ccaaggttca
     4681 ggcaattgcc agcgaactga agctgtctga tgcgcggttc gaggccctgc tggcgcaggt
     4741 acgcgacgaa ctggtgggtg ccttgtcaca cctgcccgat gccgggtcgg tagcggtggt
     4801 ggtgaaagag ctggcagcgg ctgactgaca cccaagcaag gtccgggccg gttgggcacc
     4861 aaccttgtcg gcaacgcggc tgccttgctt tgagaatcct catttttgct ttcatttgga
     4921 tagcttgcag cgcatgctgt ataagggcta gaggcctatt ttgtaccttt ttgtcaggta
     4981 tggcggcagc gctgttggtg atcaccgcac tgactcggca aatgcgccgg gtgaaggctg
     5041 gatttacttt atttttgata gctgtcagcg cacactctgt atgggctgga gccacttttg
     5101 atgaggtaaa ggcgagctgg cagccgtctg acacccgaat ccttgaccgg cacggcgaag
     5161 agctacaccg cctgcgcacc gatgcctcgg tgcgccgggg ccagtgggtg gcattggccg
     5221 atgtctcgcc ggccctgcgc accgccatgg tgctcagcga ggacaaacgt tttttcgagc
     5281 acagcggggt tgattggcag gcggtgtcag ccgccgcctg ggccaatttg tggaacacca
     5341 gaacccgcgg cgcctcgacc atcaccatgc agcttgcagg tttgctcgac gatgacctca
     5401 agcgcagtgc cggcgggcgc agcgtgcttc agaaagtctc gcaaaccgtg tccgcccaat
     5461 ggcttgagcg cagttggcgc aaagaccaga ttctggaggc ctacctgaac ctggtgtcgt
     5521 ttcgtggtga attggtcggc attgatgcgc taagccagac gctgtttggc aaagccgcac
     5581 atggccttga tgcgcgcgaa gctgctgtcg ccgctgcgct ggtgcgcgcg cccaatgcca
     5641 aaccggcgca ggtggcccaa cgcgcctgcg cggtgctggc cagcatggcg cttgccaatg
     5701 cggccaaacc agccgcagca aagccaggtt cggtcgcatc cgccagcgca acgtcagccg
     5761 gtgccagcgc agccaagcca ggcgcagcga aaccaggttc ggtcgcacct gccagcacag
     5821 cgtcagccgg tgccaacgcg gccagcagcg acgcagccgg gcgttgtgat gccctggctt
     5881 ttttcaccac tgccgccctg cagcggcgcg atttcgcgcc cagtgccggg ctggcgccgc
     5941 atctggcgcg ccaagtgctc gccacgcagg caacccgacc ggatcggcgt accaattcag
     6001 ccgctgccag caagccaact gcgggcgccc gcgctgcgac cgaaatccgc accaccctgc
     6061 ttgcgccctt gcagcggttt gccgtgcaga ccctgcagca gcacttgcgt gaactcgatg
     6121 gtcgccatgt ggaagacgct gccctgctgg tgctcgacaa cgccagtggc gaggtactgg
     6181 cctgggttgg ctcgtcgggc gagctcagtc gcgcaggcga tgtcgatgcg gttaccgcgc
     6241 tgcgccagcc cggatccacc ctgaagccct ttttgtatgc acaggcgatt gccgaaggtc
     6301 gcctgactgc ggcgtcgcta ctggaagact ccagcacgca gattcagacc agtggcggcc
     6361 tgtacattcc acaaaactac gaccgccagt tcaagggctg ggtttcggtg cgcaccgctt
     6421 tgggtgcatc gctcaatgta ccggcggtgc gcacgctggt gatggtgtcg cccgacgcgt
     6481 ttcaccagca actggtcaag ctcggcttgc cattgccaga gagcggtggc tactacggct
     6541 acagcctggc gctcggcagt gccgaggtgt cgctgctgaa cctgaccaac gcctaccgca
     6601 gcctggccaa cggcgggctt catggcccga tcagcctgag ctggcctgac gaccgcagtg
     6661 ccccgccagc ccaggctacc tcggtggcca caccgtcggc ccggcaaaag ccagcgacct
     6721 tgcgccaggt gatcgatgcg cgtgctgcct tcatcgtcag cgacatcctg tctgacccgc
     6781 tggcccgtgc ccgcaccttt ggcatggaca gtgtgctggc aacacgcttc tggacggcgg
     6841 tcaagaccgg caccagcaag gacatgcgcg acaactgggc gctgggctat tcgcagcgtt
     6901 acacggtcgg ggtctgggtt ggcaatgcca gcggcgcgcc gatgtgggat gtcagcggca
     6961 ccaccggtgc tgcgccaatc tgggcggcac tgatgaacta tctgcaccgc cagacgccca
     7021 gccaccctcc ccggccgccg cctggcgtga ttcgcaccgc cgtgcagttt gccgtcagcg
     7081 gggccagcgc caaccgaccc gtgatgccgg aggcggcgcg ccaggaatgg tttgtgcaag
     7141 gcacacaaca aaccacattc gctattaatt tggaagcttc cagcgcatat gcatcaacgg
     7201 ctggagacca aaaaggcttg aactctccgg ctcagcaagc ggtcaacgcc ggaact
//
LOCUS       DAOSLL010000172         7226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9424_length_7226_cov_4.045321, whole genome shotgun sequence.
ACCESSION   DAOSLL010000172 DAOSLL010000000
VERSION     DAOSLL010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9424_length_7226_cov_4.045321"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="PLB25_09150"
     CDS             complement(<1..386)
                     /locus_tag="PLB25_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41779.1"
                     /translation="MNQPYSHDPAYLSGLKKIEQQIQQNQLQEAALGLTKLSRNNTKD
                     PRLFLLGSLLASASGNADGTLSAARRASELAPQWHPATIYLAGVLADQGQAEEAVETA
                     ELALQQAAEGGNVSVKELSALAQRAAA"
     gene            500..2545
                     /locus_tag="PLB25_09155"
     CDS             500..2545
                     /locus_tag="PLB25_09155"
                     /inference="COORDINATES: protein motif:HMM:NF025214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE41780.1"
                     /translation="MKSQTENRLVDPSQQPKKSNSNALALLNLGLSCRAQGDLQAALA
                     HYDAAIRLQPNFADAYMARGVACNDQKDHLAAIENYKRAIALDPNLVAAHYNQANAYR
                     ALGEQQAAVNSYNQAIQLAPDLADAYANRGNALIALKKPRIALASFNRAIELKPDVAA
                     NYLNRGICFAELGDPNAAFADLGYAIKLNPLLAEAHAKRAAILAQFKHYKESIAEFEL
                     AYKAKPDLEHLLGQLLHAKNSSCDWSNYTENLDAIRLKLANNDEVIQPWANLSIFDEP
                     LLHQKCAEIWLNNIVKPGQDRKRSPEKAFIQKKKIRIGYYSPNFHDHPVMHLMNGVFQ
                     NHDQDQFELIAFSFAKKIAGDYQKNVAKSFSKLLYIDEFSDDDIVALSHDLNIDIAVD
                     LSGFTDACKPGVFLKRCAPVQINFLGYAGSLGSAQWDYIVADDQVIPPESRDFYAEKI
                     IYLPDCFQPNDVEKGISSASFSRQDFQLPQHGFVYCCSNNLYKITPPVFDVWMRVLAL
                     VPDSVMWLFSQHALGIENLKKEAQLRGVDPDRLIFARRLPSLADHLARYRLADLFLDT
                     FPYNAHTTASDALWAGLPVLTCAGQSYASRVAASLLTTIDLPELITSNHQDFEAMAVK
                     LAMQPQPLADLKQRLETNRLTTALFDTERFTRNLELAFRKALDRHAAGLPPDHIHVS"
     gene            complement(2566..3684)
                     /locus_tag="PLB25_09160"
     CDS             complement(2566..3684)
                     /locus_tag="PLB25_09160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE41781.1"
                     /translation="MSLTSSQSAPLVATANASTPTSDADAFEATFVIIPALNPSPTLL
                     TLLAELRQQGFCKILLVDDGSADAFGAIFRAAEQAGASVIRHPVNLGKGEALKTAFRS
                     AFALGAAAVVTVDADGQHSAADAARIARRVRQETSPVCVLGVRTFAAEVPLRSKFGNL
                     LTRQVFQAFSSLRVSDTQTGLRGFSGDLLLRLCQVSGHRYEFEMQMLLWLARSKLPIV
                     EVPIDTIYIDDNASSHFRPVVDSLKIYWVLFRDLFIALSSFGIDIALFSLCQALTGDI
                     LLSTYASRSISGAYNFIGNRIFVFKTIGNLRMRRELIQYILLALTLATASGGLVQVLQ
                     HATAWNVTLCKILIDLTLYGVSFLVRRLMIFKPDKKTA"
     gene            complement(3681..6083)
                     /locus_tag="PLB25_09165"
     CDS             complement(3681..6083)
                     /locus_tag="PLB25_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41782.1"
                     /translation="MAASAVGQGSVDIASYDANTQVLLLSGWAAPESPQVYTSNIVVT
                     VGNQQIYRGRFLRSIRPDVVSARQRSDWLWSGWRVEINTPTLAPGTHNVRVELLLTNG
                     ETFAIANSPSVHAIDIPPKSLPSIKSRLLLAIALLLPAMALFFPGAVQRWTKQRWPAA
                     TVFFATIVLSFLLLVASGFTGSSVRHALHDSPVLAHDAIAWLGQARFIRMDEWNILTQ
                     MAIGQVNASLHFPLLNRNVGLDGNNMLLIGMTGVPVSHVSALAKPATWGFWLFDLRGA
                     LAWHWWFPFFACFLALWAVLIRFLKVDWRIASMLAVTLPASPYSVVFSGHPAYAVFFP
                     LLSLWLLDRIFSINRPSVAAALGAMLGLSLAGFVLVLYPPWQITLLYLLVPLGVGHFV
                     SRRPAFLFGKAQFIAILLALAIALGLIGSWLIDTKEVLQTVAATVYPGQRATSVGGDI
                     DPWFAIKGLLSPITMYQETPFMNASDAGSFMWLWLPLVAACGLVCWNDRKLHLVPVLI
                     VGFLGLTLGYIYLGFSIPVAQYSLWGRVTSYRLDLALGLAQLFLLAWLISVKASVFGH
                     ALTAAASRLAIGVGLACAGLSVWLFGLLPVPIANIFTPGYVLLASAVLGIVSYLVMAQ
                     RPVLAAGIYCVWMLGTSMPFNPIDLAPSQVRLEPALKSALQAPGSPGGIRLAVLDMHR
                     WRMNLVAAGEPVVNAVLYYPQETLWKSLDPDGKFKTLYNRYQNLEFSTAELPGIDGFQ
                     IDALQLDLVNVTIDPRHFDFRKLGATHLLTSPRTASLLMGNSSLRHFASDGKWVLLKV
                     AA"
     gene            6541..>7226
                     /locus_tag="PLB25_09170"
     CDS             6541..>7226
                     /locus_tag="PLB25_09170"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HOE41783.1"
                     /translation="MKFADLIDGRENNLNLIRLMAASAVALSHSYNFTNQLPSEPLFK
                     WTGTLSFGYLSVFIFFVISGFLVAQSYERTQSIVGFIAARALRLYPALIVVLLFSAYL
                     IGPFFTTLSLNDYLPSHEVRRYFEDNLLFKQRGVLPGVFRDGGFTAGVNGSLWSLRYE
                     AYAYLLLLLCGVGGLISKRPVFNAFVALSLLLYAKDPFGTFLIPGDWSAVIYVPRIGF
                     VVGMLIYINRG"
BASE COUNT         1759 a         1999 c         1943 g         1525 t
ORIGIN      
        1 gcagcagccc gctgcgccag agcggacaac tctttgacag aaacattgcc cccttcagcg
       61 gcctgctgga gcgccaactc ggccgtttcg actgcttctt cggcttgtcc ctggtcagcc
      121 agaacgccgg ccagataaat cgttgcagga tgccattgcg gtgccagttc gctggcccgc
      181 cgtgcggccg acaaggttcc gtcagcgttg ccggaagcgc tggccaacag cgagcccaac
      241 aaaaaaaggc gcggatcttt ggtgttgttt ctcgacagtt tggttaaacc cagcgcggct
      301 tcctgcagtt ggttctgctg gatctgctgt tcaatttttt tcagccctga cagatacgcc
      361 gggtcgtgcg aatagggttg gttcattcct gtgcggggtt ggtctaagta gccgatgatt
      421 gtaaactgca gggtttgccg ccgatgcgcg gcgaactgcc cagacgggtg ccgtcagttc
      481 cattgccaca caccgattca tgaaaagtca aactgaaaat cgcctggttg acccatcgca
      541 gcaaccaaaa aagtcgaatt ccaatgccct ggccttgctc aacttgggcc tgtcttgccg
      601 ggcacaagga gacttgcagg cggcattggc gcattacgat gcggccatcc ggttgcagcc
      661 caatttcgct gatgcgtata tggcaagggg tgtcgcctgc aacgaccaaa aggaccacct
      721 ggcggcgatt gaaaactaca agcgcgccat tgcgctggac ccgaacctgg tcgctgccca
      781 ctacaaccag gcaaacgcct atcgcgcgct cggggaacag caggccgctg tcaacagcta
      841 caaccaggcg attcaactgg cccccgatct tgccgatgct tacgccaacc ggggcaatgc
      901 gctgattgcc ctgaaaaagc ccagaattgc gctggccagt tttaaccggg ccattgagct
      961 caagccggat gttgcggcca actatttgaa ccggggtatt tgttttgccg aacttggcga
     1021 cccgaacgcc gcctttgcag acctgggcta cgcgatcaaa ctgaacccgc tgctagccga
     1081 ggctcacgca aagcgtgctg ccattctggc gcaattcaaa cattacaaag agtccattgc
     1141 ggagtttgag ctggcctaca aggcaaagcc tgacctggaa caccttttgg ggcagttgct
     1201 gcatgccaaa aactcaagct gcgactggag caattacaca gaaaaccttg atgccatccg
     1261 attgaaactg gcaaacaacg atgaggtcat ccagccatgg gccaacttga gtattttcga
     1321 cgagccactg ttgcatcaaa aatgtgcaga aatctggctc aataacattg tcaagccagg
     1381 tcaggacaga aagagatcgc ctgaaaaggc atttatacag aagaaaaaaa tacgcattgg
     1441 ctattactcg cctaattttc acgatcaccc tgtcatgcat ctgatgaacg gtgtatttca
     1501 gaaccacgat caagatcaat tcgaattgat tgctttttca ttcgcgaaaa agattgcagg
     1561 cgattatcaa aaaaatgtcg ccaaatcatt tagcaaatta ttgtatattg atgaattttc
     1621 cgatgatgat atcgttgccc tgtcacatga tttgaatatt gatattgcag tggacctgtc
     1681 cggttttacc gatgcctgca agcccggtgt ttttttaaaa agatgcgcac ctgtccaaat
     1741 caattttttg ggttatgcgg gatcactcgg gtctgctcag tgggattaca ttgttgctga
     1801 tgatcaggtc ataccgcccg aatcaagaga tttctatgcc gagaagatca tctatttgcc
     1861 cgactgtttt caacccaatg acgtggaaaa aggaatttca agcgccagtt tctcgcgaca
     1921 ggactttcaa ctgccccagc acggcttcgt ctactgctgt tccaacaatc tgtacaagat
     1981 cacgccaccg gtgtttgatg tctggatgcg tgtgttggca ctggtgccag acagcgtgat
     2041 gtggttgttc agccaacatg cgctgggcat tgagaatctg aaaaaggagg cgcagctaag
     2101 gggtgtcgac ccggaccgtt tgatttttgc caggcgcctg ccttcgctgg cagatcacct
     2161 ggcacggtac cgtctggcgg atctgtttct ggacaccttt ccctacaacg cgcacaccac
     2221 cgccagcgac gccctgtggg cgggcttgcc agtgctgacc tgtgccggcc aaagttatgc
     2281 cagccgtgtg gcagccagct tattgaccac cattgacctg cccgaactga tcaccagcaa
     2341 tcaccaggac tttgaagcga tggcggtcaa actggcgatg cagccacaac ctctggctga
     2401 tctgaaacaa aggctcgaga caaaccggct gaccacagcc ttgtttgata ccgaacgttt
     2461 cacccgaaat ctggagttgg ctttccgcaa ggcgctggac cggcacgccg ctggcttgcc
     2521 cccggaccat attcatgtgt cttgatcctg tttgccagac gatggtcagg cagttttttt
     2581 gtcaggtttg aagatcatca accttcgcac cagaaaagat accccataca gcgtcaagtc
     2641 gatcaggatt ttgcacaagg tgacgttcca ggctgttgcg tgctgcaaga cttgaaccag
     2701 accgcctgaa gccgttgcca gagtcagcgc cagcaaaatg tactgaatca attcgcggcg
     2761 catgcgcaaa ttgccaatgg ttttaaagac aaagattctg ttgccaataa agttataagc
     2821 gccagagatt gatctcgacg cataggtgct caacaatatg tccccggtca aagcctggca
     2881 cagcgagaac aaggcaatgt cgatgccgaa cgaagacaac gcgataaaca aatctctgaa
     2941 cagtacccag taaattttca gtgaatcgac caccggacga aaatgcgagc tggcattgtc
     3001 gtcaatgtag atggtgtcaa tcggcacttc gacgataggt aacttcgagc gcgccagcca
     3061 aagcagcatt tgcatctcga attcgtatcg atggcctgac acttgacaca gtcgcaacag
     3121 caggtcacct gaaaacccgc gcagaccggt ctgggtgtca gagacgcgca gtgatgaaaa
     3181 ggcctggaag acctgccgtg tcagaaggtt gccaaatttg ctgcgcaacg gcacttctgc
     3241 cgcaaaagtg cgcacaccga gcacgcaaac cggtgaagtt tcttgccgca cgcgccgggc
     3301 aatgcgagcc gcatctgccg ccgagtgctg cccgtcggca tcgaccgtca ccacggcggc
     3361 cgccccgagg gcaaaggcgc tacgaaacgc tgtcttgagc gcctcgcctt tgccaagatt
     3421 gaccggatgc cgaattacgc tggcaccggc ctgctcggca gctcggaaga tggccccaaa
     3481 cgcgtcggct gagccatcgt cgaccagcaa aattttgcaa aagccctgct ggcgcagctc
     3541 cgccagcagg gtcagcaggg tcggcgacgg gttgagggct ggaatgatga caaaggttgc
     3601 ctcaaatgca tcagcatcag atgtgggcgt tgaggcgttg gcagtggcca caagcggcgc
     3661 ggactggctg gatgtcaaac tcatgcagcg actttcaata acacccattt gccatcgctg
     3721 gcgaaatgcc gcagactgct gttacccatc agcagggaag ccgtacgcgg cgacgtcagc
     3781 aaatgcgtgg caccgagttt acggaagtca aagtgtcgtg ggtcgatggt cacgttgacc
     3841 aggtccaact ggagtgcatc aatttggaag ccatcaatgc cgggcagttc tgcggtggaa
     3901 aattcaagat tctggtagcg gttgtacagg gtcttgaatt tgccatccgg gtccaggctc
     3961 ttccacagtg tttcctgtgg gtagtaaagc accgcgttga cgaccggttc gccagcggcc
     4021 accaggttca tccgccaccg atgcatgtca agcaccgcca agcgaatgcc accgggcgaa
     4081 cccggtgcct gcaatgccga cttcaaggcg ggttccagtc gcacctgact cggtgccaga
     4141 tcaatggggt tgaatggcat tgaagtaccg agcatccaga cgcaataaat gccagcagcc
     4201 aacaccgggc gctgcgccat caccaggtag ctgacaatgc ccaacacggc actggccagc
     4261 aacacatagc cgggggtgaa aatgttggca atcggcaccg gcaacaggcc aaatagccag
     4321 accgacaagc ctgcacaggc caggccgacc ccgatcgcca gtcgcgatgc cgcagcggtc
     4381 agtgcgtgac caaaaacaga cgctttgact gagatcagcc aggccagcaa gaacaactgg
     4441 gccaatccga gcgccaggtc cagccggtat gaggtgaccc gtccccacag tgagtattgc
     4501 gccaccggga tagaaaaacc gaggtaaata tagcccaggg tcagcccaag aaacccgacg
     4561 atcagcacag gaaccagatg cagtttgcga tcattccagc aaaccagtcc gcaggcggct
     4621 accagcggca gccagagcca catgaatgaa ccagcatcgc tggcattcat gaacggtgtt
     4681 tcctggtaca tggtgatcgg gctaagcagc cctttgatgg caaaccaggg gtcgatgtcg
     4741 ccgccgaccg aggtcgccct ttgtccggga tacaccgtgg cagccaccgt ctgcagcacc
     4801 tctttggtgt cgatcagcca actgcctatc aaccccaggg caatcgccag tgccagcaaa
     4861 atcgcgatga attgcgcctt gccaaacaaa aatgctggtc tgcgtgagac aaaatggcct
     4921 acccccaacg gcaccagcag gtacagcagg gtaatttgcc aaggcgggta taagaccagg
     4981 acaaacccgg ccagtgacaa cccgagcatg gcacccaaag cggccgccac agacggccga
     5041 ttgatgctga aaatcctgtc gagcagccac aggctcagca gcgggaaaaa gactgcatag
     5101 gccggatgcc cggaaaacac caccgagtag ggcgacgcgg gcaaggtgac cgcaagcatc
     5161 gaggcgattc gccaatcgac cttcaaaaag cggatcaaaa cagcccacag ggcgagaaaa
     5221 caggcaaaaa acggaaacca ccaatgccag gccagggcac cacgcaaatc aaacagccag
     5281 aacccccaag tagccggctt ggctaacgcc gacacatgag acaccggaac accggtcatg
     5341 ccgatcagca gcatgttgtt gccgtcaaga ccaacgttgc ggttgagcaa ggggaaatgc
     5401 agcgacgcat tgacttgccc aatggccatt tgcgtcagaa tgttccattc gtccatgcgg
     5461 atgaacctgg cctgccctag ccacgcaatg gcgtcgtggg ccaagaccgg tgagtcatgc
     5521 agggcgtggc gcaccgacga accggtgaag ccagaggcga ccaacaacag aaatgacagg
     5581 acgattgtgg cgaaaaaaac cgttgccgca ggccagcgtt gtttggtcca gcgttgcacc
     5641 gcgcccggaa agaaaagcgc catcgctggc agcagcagtg cgattgccag caacaaccgc
     5701 gacttgatcg aaggcaaact cttgggcggg atgtcaatgg catggactga tgggctgttg
     5761 gcaatggcaa aagtctcccc attggtcaac aacaattcaa cccgcacatt gtgtgttcca
     5821 ggggccagag tcggggtgtt gatttccacc cgccagccgg accacaacca gtcagaccgc
     5881 tgcctggcac tcacaacgtc gggtcgtatc gaccgcagaa acctgccacg gtagatctgc
     5941 tgattgccta cagtcacaac aatgttcgag gtgtaaacct gcggtgactc aggcgcggcc
     6001 cagccactca acaacagcac ttgggtgttc gcgtcgtagc tggcgatgtc cacgctgccc
     6061 tggccgacgg cactggcagc catcgccgat gcagcgcaaa accacagtcc aaaccacagg
     6121 caggttcgac cggacgcgac ccgcccaatc gccatcttat aaaaaaggaa tttcttgact
     6181 atttccactg gtccattgat cgaatgccga tttcctctgg caagttgaga tgctgcaagt
     6241 gtctggtaag cgcttatcga tacctttcgg gggcaaaaga gtttcagcct ttatctgcag
     6301 ccagtgaggc tacgctgatc gcttcaaggc aagcgcttga catgcatctc gcaaagttcc
     6361 tattgtcgcc gacaacatgc gggtcaactt gtcttgccgc gaagcctgag cggtcgggca
     6421 cagcagggaa cacaaaagga aacttaccct attggcaagg actcgctgca agcacttgaa
     6481 caaggtggcc tgctagcctg aaagcccatc gcatgccagg aaaccgggca cgagaacacc
     6541 atgaaatttg ctgacttgat tgacggacga gaaaacaatc tgaatctgat cagactgatg
     6601 gcagcgtctg ccgtggcgtt gtcgcattcc tacaatttca ccaatcaact tccttctgag
     6661 ccattgttca aatggactgg taccctctcc ttcgggtatt tatcggtatt catttttttt
     6721 gtcatcagcg gttttctggt ggcgcaaagt tatgagagaa cgcagagtat tgtcggtttt
     6781 attgcagctc gggctttgcg cctgtatccg gcattgatcg ttgtgctgtt gtttagcgcc
     6841 tatttaattg ggccgttttt cactaccttg agtttaaatg attacctccc aagtcacgaa
     6901 gttcgccgtt atttcgagga caaccttctt ttcaaacagc gcggcgtctt gcccggtgtt
     6961 tttcgtgatg gtggattcac cgccggggtc aatggttcac tctggagctt gcgatatgag
     7021 gcgtatgctt acctgctttt gctgctctgc ggcgtcggtg gcctgatatc gaagcgcccg
     7081 gtatttaatg catttgtggc gctctcactg ctgctttatg ccaaggaccc gtttggtacc
     7141 tttttgattc caggtgactg gtctgctgtt atttatgttc cgagaattgg ttttgtagtt
     7201 gggatgttga tttatattaa ccgtgg
//
LOCUS       DAOSLL010000173         7162 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9517_length_7162_cov_3.839595, whole genome shotgun sequence.
ACCESSION   DAOSLL010000173 DAOSLL010000000
VERSION     DAOSLL010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7162
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9517_length_7162_cov_3.839595"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLB25_09175"
     CDS             complement(<1..649)
                     /locus_tag="PLB25_09175"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HOE41784.1"
                     /translation="MKPVSSVFQWFFLAWRGRLAALLVLAGVTLASLGSEVDLPPAWQ
                     GSVLARLLGLAGTPFAAARQALFDGYQQAMPRTTSSQPVTLVEIDEDSLKWVGQWPWP
                     RNRLADLIDKIAVHQPLAIGLDLYMPELDQTSPDQVAGHLPSGHEALALALKQLGSHE
                     ARLIESLKAAPTVLGAAGFGSQTQTSSNGLRSVPVLVSGGDALPYLVNFPWVLASL"
     gene            complement(737..1693)
                     /locus_tag="PLB25_09180"
     CDS             complement(737..1693)
                     /locus_tag="PLB25_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE41785.1"
                     /translation="MKLQFWGTRGSIPVSMTSVDVRTKLRQALLGAAQGPVLTADSVD
                     AFIDTLGFDVAATFGGHSSCVQIVTDNPEHVILDLGSGARPLGQSMLARFGVDQPQTY
                     HVFMSHVHWDHIMGFPFFLPAFTPGNRIIIHACHAQVEQAFRRQHGSPSFPVEFEQLA
                     ATVEFDVLLPGKRYQIAGLEVSARLQRHSGDSYGWRFERGGKAVVYSTDSEHRLDEAA
                     ELAGIVEFFANADAVVFDAMYSLADAISVKADWGHSSNIVGVELCRAAGVRRLVMFHH
                     EPAYDDAQILGVLNETRRFEEITREGHALEVLSAWDGLELAL"
     gene            complement(1690..2037)
                     /locus_tag="PLB25_09185"
     CDS             complement(1690..2037)
                     /locus_tag="PLB25_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008448756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOE41786.1"
                     /translation="MELRSQTRGVALVLTVIGRIDHSHANAFEAALAPHLQLCTASGT
                     PLVLDFSGVDFISSVGLRVLMLAAKQVKQQQGKLAVAALTPIVQEVFQVSRFNLVLKV
                     FASVDQAVLALTA"
     gene            2262..3965
                     /locus_tag="PLB25_09190"
     CDS             2262..3965
                     /locus_tag="PLB25_09190"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE41787.1"
                     /translation="MQVNFLAQQTLLTHVIRSSLAIGLVSLWGLFGYASFVLQSDLAQ
                     QPGLQHRLLLTTVLLTLLAAGLSWWLLRAQLAAATNTAQALAEPAQQEQPVSAASLPK
                     PGEMGQPVSAFNTPQDRLAQQAQQAQQAQQALAQSQATLARTEAIVGMGSWQWDLATQ
                     TMSWSDGMFGLMGADAGSTAPALADQARFYEASDFQQLQNAVATTQGTGEPFALELRV
                     VRADGTQRICLARGQAERGPDQQVLRLNGSLQDITEQKQTDLKLQQAASIFRHAQEGI
                     TVTDLNGTILDVNLAFSRITGYQPEDALGNNPRMHKSDRQDASFYQSMWRELKRSGHW
                     SGEVWNRRKNGEVYAEWLHICPVRDENGIVLRYVGLFTDISARQANADPVRKLAFFDA
                     LTGLPNRRLLTDRLGQAMLLNQRSGHQGAVILLDLDGFKPLNETHDHPTGDLLLIEVA
                     RRLKACVRQVDTVARICGDEYVVLLSELSADMQRSTEQARTLAENVRQCLAGPYLLTL
                     QHQGQPDVTVEHRCSASVGVVMFGPTESDQELVLKRADSAMYKAKAAGRNRVVFYQGS
                     A"
     gene            complement(4174..4863)
                     /gene="elbB"
                     /locus_tag="PLB25_09195"
     CDS             complement(4174..4863)
                     /gene="elbB"
                     /locus_tag="PLB25_09195"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HOE41788.1"
                     /translation="MKRVENPPKVAVLLAGCGHLDGAEVREAVLTLLALDQHAAAVQC
                     VAPNAPQYHVVNHLTGEPEAGAQRNILEESSRISRLGKCLDLAQARVQDFDALLMPGG
                     YGVAKNHCSFAFKGVNAEVRPDVAAFVGGFFAAGKPVGAICIAPALVALALHASHDSA
                     AMTLGNDTGVADAITQLGQQFTSTPSAREVVIDEAHKLVTTPAYMFDDALLSDVFVGI
                     GRCVAEVLKRV"
     gene            complement(4943..6877)
                     /locus_tag="PLB25_09200"
     CDS             complement(4943..6877)
                     /locus_tag="PLB25_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HOE41789.1"
                     /translation="MRSPPTALALRQARLHLLEHGDCPPDGLDEHVARSWRRSLAAGL
                     LPAGRLLESEHSSGAELTRTLAGNHELLAHSRPVMEYLFEQVRHTQGMVILADQRGTL
                     IHTLGHADFLSRADRVALTSGANWHEQQRGTNAIGTALVEAMGVEIHGAEHFLERNEF
                     LTCTAMPILSAVGELMGILDISGDQRSRHPHTLGLVGMASRMIENRLVSTSCQRHIRL
                     HLHPQLEGIGSVAEGIVALTEDGWLVGANRAAMSLLQLSAAELGAVQLQQRLDLPLAQ
                     LLSRHQRRPGVPTQVRRLDGTALFVLVQRDVAALVSHPAAVPDIKLPNSPSPDALRSL
                     DTGDARWRAAADKVRRVLDKPIPLLIQGESGVGKELFARAAHDSGPRRSKPFVAINCA
                     AVPENLIEAELFGYAPGAFTGARREGSPGRLREAHGGTLFLDEIGDMPLPMQARLLRV
                     LQERQVTPLGGGQAVSVDFALICASHRKLREEAATGRFRNDLYYRINGLTVLLPPLRE
                     RSDFAVLTQRLLADLNPGHTVDVKPDLMQRLSQHGWPGNLRQYANVLRTASAMLDPHE
                     SRIDWVHLPDDLLEDLAASVVPDGLAANPPAGAVLAQNLQALSRAAIRQALDSSRGNI
                     SQAARDLGISRQTLYRKLGD"
BASE COUNT         1484 a         2275 c         2158 g         1245 t
ORIGIN      
        1 gcaagcttgc cagcacccaa gggaaattca ccagataggg caaggcatcc ccaccgctca
       61 ccagcaccgg caccgagcgc aagccgttgc tgctggtctg ggtttggctg ccaaagccag
      121 ccgccccaag caccgtgggg gcagctttca gcgactcgat caggcgggcc tcgtggctgc
      181 ccagttgctt caacgccagc gccagcgctt cgtggccgct cggcaaatgc ccggccacct
      241 ggtccggtga ggtttggtca agctcgggca tgtacaggtc aagcccgatg gccaacggct
      301 gatgcacggc aatcttgtcg atcagatcgg ccagccggtt gcgtggccag ggccattggc
      361 cgacccactt caggctgtcc tcgtcaattt ccaccagcgt caccggctgg ctgctggtgg
      421 tgcgcggcat cgcctgctga tagccgtcaa atagcgcctg ccgcgcggcg gcaaacggtg
      481 tcccggccag acccagcagg cgggccagca cgctgccctg ccaggccggc ggcaaatcaa
      541 cttcactgcc caggctggca agggtcacgc cagccagcac cagcaaggcc gccaaccgcc
      601 cgcgccaggc cagaaaaaac cactgaaaca cagaggaaac cggcttcaac ccggatacct
      661 cagttgaccg ctggttttca tctgcaagcc cctatgagtg tttacttttt ggcttcgatg
      721 gggttcactt tttgggtcac agcgccaact ccaggccgtc ccaggctgac aagacttcca
      781 gcgcgtgacc ctcgcgagtg atttcttcaa agcggcgtgt ctcgttgagc acacccaaaa
      841 tctgggcatc gtcataggcg ggttcgtggt gaaacatcac cagccggcgc acaccggccg
      901 cccggcacag ctcaacgccg acgatgttgc tggagtgccc ccagtcggcc ttgaccgaaa
      961 tggcatcggc cagggagtac atggcatcaa acaccacggc gtcggcattg gcaaaaaatt
     1021 ccacaatgcc tgccagttcg gcagcctcat caagccggtg ctcggaatcg gttgaataga
     1081 ccaccgcttt gccaccccgc tcaaaccgcc agccgtaaga gtcgcccgag tgccgctgca
     1141 accgtgccga cacctcaagc ccggcaatct gatagcgctt gccgggcaac agcacatcaa
     1201 actcaaccgt tgcggcaagt tgctcaaact cgaccggaaa cgacgggctg ccatgctgac
     1261 gcctgaaagc ttgttccacc tgtgcgtgac aggcgtggat gatgatgcgg ttgcctggcg
     1321 tgaacgcggg caggaaaaac ggaaaaccca tgatgtgatc ccaatgcacg tgggacatga
     1381 acacatggta ggtctgcggc tgatccacgc caaaacgcgc cagcatcgac tgccccagag
     1441 gccgggcacc cgagcccagg tccaaaatga cgtgctccgg gttgtcggtg acgatctgca
     1501 cacaactgga atgaccacca aaggtggccg ccacatcaaa acccagggtg tcaataaagg
     1561 catcgacgct gtcggcggtc aggactgggc cttgcgccgc acccagcaag gcctggcgca
     1621 gcttggttcg cacgtcaacg ctggtcatcg acaccggaat tgagccccgc gtgccccaaa
     1681 actgaagttt catgctgtca gcgccagcac cgcctgatcg acgctggcaa acaccttgag
     1741 gaccaggttg aaacggctga cctgaaaaac ctcctggaca atcggggtca gcgccgccac
     1801 ggccagcttt ccctgttgct gcttaacctg tttggcagcc agcatcagca cccgcaggcc
     1861 aacgctgctg atgaaatcga cgcctgaaaa atcaagcacc aagggggtgc ctgatgccgt
     1921 gcaaagctgc aaatggggtg ccagcgcagc ctcaaacgca ttggcatgcg agtggtcaat
     1981 gcgaccaatg acggttaaca ccagcgccac accccgggtt tgtgatctga gttccaacgt
     2041 atcctcgcaa agcttgaatt gacgcgacaa gtatatcgac gcactgtcag caggtggctc
     2101 tgttgacctg gcccaggcaa cgcctgtgac cacaaatagc tatcaaaaaa atagctactc
     2161 gcgcagacag cataggcgct agaggcgtaa aaggcatgca aatccgataa aaagcttcgg
     2221 gattactgcc aaagtgcccc gctggcagta gcattgcgcc catgcaagtc aattttctgg
     2281 cgcaacagac actgttaacg cacgtcattc ggtcttcgct ggccattggt ctggttagct
     2341 tgtggggctt gttcggttat gccagtttcg tgctgcaaag cgacttggcg cagcagcccg
     2401 gcttgcagca ccggctgttg ttgaccaccg tgctgctgac cctgctggct gctggcctga
     2461 gctggtggtt gctgcgcgcg caattggcag cggcgaccaa caccgcacag gcgcttgccg
     2521 aaccggcgca gcaagagcag ccggtgtcag ccgcatcgct gccgaagcca ggggaaatgg
     2581 gacaaccggt gagcgccttc aacaccccgc aggaccggct ggcgcagcag gcgcagcagg
     2641 cgcagcaggc gcagcaggcg ctggcccaaa gccaggccac gctggcaagg accgaagcca
     2701 ttgttggcat gggcagttgg cagtgggacc tggcaaccca gaccatgtcc tggtctgacg
     2761 gcatgttcgg cttgatgggt gctgacgctg gcagcacggc accagcattg gccgatcagg
     2821 cccgctttta cgaggccagc gacttccagc agttgcaaaa cgcagtggcg acaacgcaag
     2881 gcaccggaga gcccttcgcg ctggagttgc gcgtcgttcg cgctgatgga acacaacgca
     2941 tttgcctggc acgcgggcag gccgagcggg ggccggacca gcaggtgctg cggctgaacg
     3001 gatctttgca agacatcacc gaacaaaagc aaaccgattt gaagctgcaa caggcggcca
     3061 gcatttttcg ccacgcccag gagggcatca ccgtcaccga cctgaacggc accattctgg
     3121 acgtgaacct ggcatttagc cgcatcaccg gctaccaacc ggaagatgcc ttgggaaaca
     3181 acccgcgcat gcacaagtca gaccggcagg acgcaagttt ttaccagtcg atgtggcgcg
     3241 agttgaagcg ctcgggtcac tggagcggcg aagtctggaa ccggcgcaag aacggcgagg
     3301 tctatgcaga gtggctgcac atctgtccgg tgcgcgatga gaacggcatc gtcttgcgct
     3361 atgtcgggtt gtttacagac atctcggcac gccaggcaaa tgcagacccg gtgcgcaagc
     3421 tggctttttt tgatgccctg accggcttgc caaaccggcg tctgctgacc gaccggctgg
     3481 gccaggcgat gttgctcaac cagcgcagtg gccaccaggg tgccgtgata ttgctcgacc
     3541 tggatggttt caagccgctc aacgaaaccc atgaccaccc aaccggcgac ctgctgctga
     3601 tcgaggtggc ccgccgcctg aaagcctgtg tgcgccaggt ggacaccgtg gcacgcattt
     3661 gcggcgacga atatgtggtg ctgctcagcg aactcagcgc cgacatgcag cgctccaccg
     3721 agcaggcccg aaccctggcc gagaatgttc ggcagtgtct ggccgggcct tatctgctaa
     3781 cgctgcaaca tcagggtcag cccgatgtga ccgttgagca ccgctgcagc gccagcgtcg
     3841 gcgtggtcat gtttggcccg accgagagtg atcaggagct tgtgctcaag cgcgccgaca
     3901 gcgccatgta caaggccaag gcggccgggc gcaaccgtgt ggtattttac cagggttctg
     3961 cctgaagcta tactaactat agctgctaac gcaatgtgca taagcgccgc agcctttttt
     4021 cactgccaaa tcagtggtac ccagacggcc tttcgcagcc accagcaggc acttgctgca
     4081 tcgcatcaat gcaggcattg gcaaggcaat ggctgcaagt ctgaaccggc tgatgtgatg
     4141 ccttgacccg gatgcccgcc gcaggggcgc gggttacacc cgtttgagca cttcggccac
     4201 gcagcgcccg atgccgacaa acacatcgct caacagcgcg tcgtcaaaca tgtaggccgg
     4261 cgtggtcacc agtttgtggg cctcgtcaat gaccacctca cgggcgctgg gcgtgctggt
     4321 gaattgctgg ccgagctggg tgatggcatc tgccacacca gtgtcattgc ccagcgtcat
     4381 ggcggcactg tcgtgactgg catgcagcgc cagcgccacc agtgccggtg caatgcagat
     4441 cgcaccgact ggcttgccgg ctgcaaaaaa gccgccgaca aacgcggcca catcgggacg
     4501 cacctcggca ttgaccccct tgaaagcaaa cgagcaatgg tttttggcca ccccataacc
     4561 acccggcatc agcagcgcat caaagtcctg cacccgggcc tgcgccaggt ccaggcactt
     4621 gcccaggcgc gaaatgcggc tggactcttc caggatattg cgctgtgcac ccgcctccgg
     4681 ctcgccggtg aggtggttga ccacgtggta ctgcggcgca ttgggcgcga cgcactgcac
     4741 cgctgcggca tgctggtcca gcgccagcaa ggtcaacacg gcctcgcgca cttcagcacc
     4801 gtcgagatga ccacaaccgg ccagcaagac agccaccttg gggggatttt caacacgttt
     4861 catagagatc tccttgaaat caaagacatt atcacgcgcc aggccagtgc aaatacacca
     4921 ctttcatgac gattggcagc gctcaatcgc caagcttgcg gtacagcgtc tgccgactga
     4981 tgcccaggtc tctggccgct tgcgagatat tgccacggct gctgtcgagc gcctggcgga
     5041 tggcggcacg cgacagggcc tgaaggtttt gggccaaaac ggcaccagcc ggtggattgg
     5101 ctgcgaggcc gtccggcacc acggatgccg ccaggtcctc cagcaaatca tcgggcaggt
     5161 ggacccagtc aatgcggctt tcatgcgggt ccagcatggc gctggcggta cgcagcacgt
     5221 tggcatactg gcgcaggttg cccggccagc cgtgctggct caggcgctgc atcagatcgg
     5281 gtttgacatc gaccgtgtga ccagggttca ggtcggccag cagtcgctgc gtcaacacgg
     5341 caaagtcgct gcgctcgcgc agtggcggca gcagcaccgt caagccattg atgcggtaat
     5401 acaagtcatt gcgaaagcgc ccggtggcgg cttcttcgcg caatttgcgg tggctcgcgc
     5461 aaatcagggc gaagtccacc gacacggcct gaccaccgcc cagcggcgtt acctggcgct
     5521 cctgcagcac ccgcagcagt cgcgcctgca ttggcaaggg catgtcacca atttcatcga
     5581 gaaacaaggt gccaccatgg gcttcgcgca ggcgccccgg actgccctca cgacgggctc
     5641 cggtaaaggc acctggtgca taaccaaaca gctcggcctc gatcagattc tcgggaacag
     5701 ccgcgcagtt gatcgccaca aagggcttgc tgcggcgcgg accactgtca tgcgcggcac
     5761 gggcaaacag ctccttgccg acacccgatt cgccctgaat cagcagcggg atcggcttgt
     5821 cgagcacccg gcgcaccttg tcggcggcag cgcgccagcg tgcatcaccg gtgtccaggc
     5881 tgcgcagggc atcgggactt gggctgtttg gcagcttgat atctggcact gccgccggat
     5941 ggctgaccag cgcagccacg tcgcgctgca ccagcacaaa cagcgctgtg ccgtccagtc
     6001 ggcgcacctg ggtcggcacg cctgggcggc gctggtggcg cgacagcaat tgcgcaagcg
     6061 gcagatcaag gcgttgctgc agttgcacgg caccgagttc agccgccgac aactgcaaca
     6121 gactcatcgc ggcgcggttg gccccgacca gccagccgtc ctcggtcagc gccacgatgc
     6181 cctcggcgac cgaaccaatg ccttcaagct gcggatgcag atgcaggcga atgtggcgct
     6241 ggcaggaggt gctgaccagc cggttttcaa tcatgcgcga ggccatgccg accagcccca
     6301 gcgtgtgcgg atggcggctg cgctggtctc ccgaaatgtc cagaatcccc atcagctcgc
     6361 cgactgccga caggatcggc atggcagtgc aggtcaggaa ctcgttgcgc tccagaaaat
     6421 gctcggcccc atgaatttca acccccatgg cttcaaccag agcggtgcca atggcgttgg
     6481 tgccgcgctg ctgctcatgc cagttggccc cgctggtcag tgccacgcgg tccgcccggc
     6541 tcagaaaatc ggcatgcccc agggtgtgga tcagagtgcc acgctggtcc gccaaaataa
     6601 ccatgccctg cgtgtggcgt acctgctcaa acagatattc catgaccggg cgcgaatgcg
     6661 ccagcagctc atggttgcca gccagggtgc gggtcagttc ggcaccgctg ctgtgctcgg
     6721 actcaagcaa acgcccggcc ggcagcagcc ctgccgccag gctgcgccgc catgagcggg
     6781 ccacatgctc gtccagcccg tcgggcgggc agtcgccatg ctcaagcaga tgcaagcggg
     6841 cctggcgcaa ggccaaggcg gttgggggtg atcgcacgtt gcctcctggc ggactggtgt
     6901 ctgctctgca atgaagtgaa ttgggcgatg ccaaggtgct ggcgacatcc ggcagcttgc
     6961 atgacagcgc cctggcagca cctgtcgcgt ttgcgtacag gtgtcccata ttgcaacact
     7021 tgccaatgcc ggacaacaca gggcaaaccc tgatacaaaa tcctgtactg cttgtaaaac
     7081 catttcaaat caagcacttg caatgagcca cgcaggctgg cacggccctt gatagttgac
     7141 attgcccgca agggaaccca at
//
LOCUS       DAOSLL010000174         7128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9569_length_7128_cov_4.055139, whole genome shotgun sequence.
ACCESSION   DAOSLL010000174 DAOSLL010000000
VERSION     DAOSLL010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7128
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9569_length_7128_cov_4.055139"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="PLB25_09205"
     CDS             <1..218
                     /locus_tag="PLB25_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41790.1"
                     /translation="RTGKDWASADRACLELQVNKRMRYVHRIPGLFFTVFSVFFKQIF
                     GELPLQGSCFQDVQAQNIRRPVDGLSA"
     gene            244..2784
                     /locus_tag="PLB25_09210"
     CDS             244..2784
                     /locus_tag="PLB25_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE41791.1"
                     /translation="MFCAALLLPGGALQAGVAIAPQAAARQVRVGVYANPPKIMLGDN
                     GQPSGMLGDLLVAIAERERWKLQAVVCEWQDCLDSLQAGGIDLMPDVAFTDQRGQIFD
                     FHNTAALLSWSQLYKARGEPISSMLDLNGKRVAVVTGSVQETALKSMLQSFGVQAELV
                     PVESFVAGFEMTAARLADAVASNRFTGDLQAPRFKLEPTAIVFQPAQLYYATGKGKNA
                     ELLTAIDHHLALWQVQSDSPYTQALHRWLQAPLRFSLPGYFWWALGTGMVLLLLALLA
                     ATWLRRQVVQKTAGLVASEDRLNTILDSVDAFIYIKDTELRYQYANSRVCALFDQSLE
                     AVVGQTDSVFFDAATATKLQVNDLRVIERGERVEEEEINRSPDGLTEHSFLSIKLPLR
                     RPDGSIYALCGISTDISKRKQAEQAMHQLAFYDPLTGLPNRRLLRDRLQQMLANLKRV
                     PQGAALLFIDVDNFKDINDTLGHDAGDALLCRMAGRIGTCIRAQDTLARQGGDEFVVL
                     LVDLSPSPTEAARQAQQVGLKILECLRAPFALGTLTEQPCQSSVSVGVALIDGQSGSR
                     DELFKQADLAMYQAKASGRNTLRFFNPAMQAEVLARTSLETDLQRALLAQEFVLFYQP
                     QVGVDGQTLGYEALVRWQHPTRGLVPPSAFIAAAEACGVILPLGRWILDSACRQLLTW
                     ASQTASAHWTIAVNVSAQQFRQADFVAQVQAALTQTGANARRLELELTESQLVDEVEV
                     VIARMMALKQLGVRLSLDDFGTGYSSLNMLKRLPLNQLKVDQSFVRDMLKSPQDASII
                     RAIVTMGDSLSLEVIAEGVELPEQRDALLALGCRHFQGYLYGRPTPLT"
     gene            2939..3073
                     /locus_tag="PLB25_09215"
     CDS             2939..3073
                     /locus_tag="PLB25_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41792.1"
                     /translation="MGKEGFDGKCQARHKSRGATGYTSGLQQRMALVDATELFKSFFA
                     "
     gene            complement(3078..3707)
                     /locus_tag="PLB25_09220"
     CDS             complement(3078..3707)
                     /locus_tag="PLB25_09220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41793.1"
                     /translation="MLRFFQVLLTTVVLLHALAAQAAGDVGIFDRLSGEVSVQSSSGS
                     SFRALAFMKVRDGDLVTVAAGGLAQIVYFDARKRELWQGPSSFRAGTAGGTRVTGHVA
                     TVSEVKGVPSRDSLAFAGNVQRLGSLTLRGVHLNPDNATIAKAHADYLVWTAAAAPDD
                     LLPELSMLGLVRERQDPALYAPYLEALQRKQPDSPEVKALVRQYRSSKP"
     gene            complement(3750..5579)
                     /locus_tag="PLB25_09225"
     CDS             complement(3750..5579)
                     /locus_tag="PLB25_09225"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOE41794.1"
                     /translation="MPALRHRWLAVASASLTAALATLIAPLLPWMAAMDLFVFDAWHR
                     LAGQRTEAMHTAIVAIDDTSLARFHDTPLAFWQPQFGAAIATLRQAGVKVIGIDLIQA
                     SSAEAWLNVLGAGDTPAGRSYEAPYRAALADGQVVLAGARAGQPGEADLMPPAEHIAL
                     LPGGADDIGLVTLEADGDGFVRRLAPVLASNSPSLAFAARLVLAAQPERQFASAALTI
                     PFAGPPGTVPRVSLATLTDPDALKQPQVQALRDRIVIIAADATQLQDLQLTPYARALP
                     GLPPQLMSGGEVHAQVVEAWLGQRTIAVPAAWQGPLLAAALGLLLGAVALRSTPILLA
                     SLAALLAVASLLLSAALFGHDVWLAPTPLLLGLASAWTVGLGNRAGREARERAHLRRL
                     FSRYVSDEVAELAVRDALPDTDGAAADVTVLFLDIRDFTSISEKLSSREVFELLNAWL
                     PQACDPILAAHGNVDKFIGDAVMAVFGSPLPLPDHAQRAIDAARTVALAADEFAVWVA
                     TRFADRKLPPFAIGIGLHSGPVVVGNLGTAGRIEFTAIGDTVNVASRLESASKTLGWR
                     IVASAATVQAAGSGLRLGASQSVSLKGKASAIEVFEIPYHPET"
     gene            6146..7117
                     /locus_tag="PLB25_09230"
     CDS             6146..7117
                     /locus_tag="PLB25_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012876.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HOE41795.1"
                     /translation="MNIRQLIVLLIVLAVGGMGFGGGIAAFMSRGSASEVKTVTEGVV
                     PSALASVELMSQLKDVQIATLAMVSAPDADAVAKAQQDLSGRKALLQEALRLQSVQAD
                     SNAQRGLITQAQESLVNYFASIDDTAKFRIAGQKEMAEAFLGATVDQYLREQMSIIET
                     IQIEKRRSKDEAILRMNDNLRASTTTLLIITLVSVAMMGMVGLLLYRRIIHPINDMQS
                     KMTDIATSQDFSHRVPVKRSDEIGRSITAFNTMIEKIQESSELVKQKTADIQAMLHYI
                     PQGILTVMPGNKVHPEYSSYLETILETREIGGQDGFFVVLCGSCIEK"
BASE COUNT         1424 a         2136 c         2195 g         1373 t
ORIGIN      
        1 ctcggaccgg taaggattgg gcaagcgctg atcgggcatg tttggaactc caagtgaaca
       61 aacgaatgcg ctatgttcat cgtatacccg gtttattttt cactgttttt tccgtctttt
      121 tcaaacaaat atttggcgag ctgccgctgc aaggctcttg cttccaagat gtgcaggccc
      181 aaaacatccg ccgtcctgtt gatggtttgt ctgcctgact ggctcaagtc ctgctgccaa
      241 ctgatgtttt gcgccgcttt gctgctgccg ggtggcgcac tgcaggccgg cgtggctatt
      301 gcgccacagg ccgcagcgcg ccaggtcagg gttggtgtct atgccaatcc accgaaaatc
      361 atgctgggtg acaacggcca gcccagtggc atgctgggcg atctgctggt ggccattgcc
      421 gagcgcgagc gctggaagct gcaggcggtg gtgtgtgaat ggcaggactg tctggactcc
      481 ctgcaggcgg gcggcatcga cttgatgcct gatgtggcat tcactgacca gcgcgggcag
      541 atctttgact ttcacaacac ggcggccctg ctgagctggt cgcagttgta caaggcacgc
      601 ggtgaaccga tcagctcgat gcttgacctg aacggcaagc gggtggcagt ggtgaccggt
      661 tcagtgcagg aaacggcgct caaaagcatg ctgcaaagct ttggcgtgca agccgaactg
      721 gtgccggttg agtcatttgt tgccggtttc gagatgacgg cggcgcgcct ggccgatgcg
      781 gtggccagca accggttcac cggtgacctg caagcccccc gcttcaagct ggagccgacc
      841 gccattgtgt ttcagccagc acaactgtat tacgccaccg gcaaaggcaa aaacgccgag
      901 ctgctcacgg ccatcgacca tcatctggcg ttgtggcagg tgcaaagtga ttcaccctat
      961 acgcaggcgc tgcatcgctg gttgcaggca ccgttgcgct ttagcctgcc aggctatttc
     1021 tggtgggcgc tcgggactgg catggtgcta ctgctgctgg cgctgctggc cgccacctgg
     1081 ctgcgccgcc aggtggtaca aaaaaccgca ggattggttg ccagcgaaga ccggctcaac
     1141 accattctcg acagtgtcga tgccttcatc tacatcaagg acaccgagtt gcgttaccaa
     1201 tatgccaata gccgtgtctg cgccctgttt gatcagtcct tggaggcagt ggtaggacag
     1261 actgacagcg ttttttttga cgccgctacg gccaccaagc tgcaggtcaa tgacttgcgg
     1321 gtgattgagc gcggcgagcg ggtggaagaa gaggaaatca accgcagccc cgacggcctg
     1381 accgagcaca gtttcctgtc catcaaactg ccgctgcgcc gcccggatgg cagcatttac
     1441 gcgctgtgcg gcatctccac cgacatcagc aagcgcaagc aggccgagca ggcgatgcac
     1501 caactggcct tttacgaccc gctcaccggc ctgccaaacc ggcgcctgct gcgcgaccga
     1561 ctgcaacaaa tgctggccaa cctgaagcgg gtgccgcagg gtgccgcgtt gctgttcatt
     1621 gacgtggaca acttcaagga catcaacgat accctgggtc acgacgcggg cgatgcgctg
     1681 ttgtgccgga tggccgggcg cattggcacc tgcattcggg cgcaagacac gctggcgcgc
     1741 cagggtggcg atgaatttgt ggtgctgctg gtggacctga gcccttcacc caccgaggcg
     1801 gcgcgtcagg cgcaacaggt tgggctgaag atactggaat gcctgcgcgc ccccttcgca
     1861 ctggggactc tgaccgaaca gccctgccaa agcagcgtca gtgtcggggt ggcgctgatt
     1921 gacgggcagt ccggcagccg cgacgagctg ttcaaacaag ctgatctggc catgtaccag
     1981 gccaaggcca gcgggcgcaa cacgctgcgt tttttcaacc ccgccatgca ggccgaagtg
     2041 ctggcccgca cctcgctgga gaccgacttg cagcgcgcct tgctggcgca ggaattcgtt
     2101 ctgttttacc agccccaggt gggtgttgac gggcaaacgc tgggctatga ggcgctggtg
     2161 cgctggcaac acccgacgcg cggcctggtg ccgcccagcg ccttcattgc ggcggcagaa
     2221 gcttgcggcg tcattctgcc gctgggtcgc tggattctgg acagtgcctg tcgccagttg
     2281 ctgacctggg catcgcagac ggccagcgcg cactggacca ttgcggtcaa tgtcagcgcg
     2341 caacagttcc gccaggcaga ctttgtcgcg caagtgcagg cggcgctgac gcagaccggt
     2401 gccaacgcca ggcggcttga gctggagttg accgaaagcc agttggtgga cgaagttgag
     2461 gttgtcatcg ccaggatgat ggcgctcaaa cagctcggtg tgcgcttgtc attggatgac
     2521 tttggcaccg gctattcgtc gctcaacatg ctcaagcgtc tgccgctgaa ccagctcaag
     2581 gtggatcagt catttgtgcg cgacatgctc aagtccccgc aagatgccag catcatccgc
     2641 gccatcgtca ccatgggaga cagcctgtcg ctggaggtga ttgccgaagg cgttgaactg
     2701 ccggagcagc gcgatgccct gctggcactg ggctgtcgcc attttcaggg ctacctgtat
     2761 ggcaggccga cccctttgac ctagcagcct agcggactga tcggccaacg gctgcgaacc
     2821 gatcacagcg gcgctaagct atacaagcgt cttgcaacga cgctgtgcgc ccaaatccgc
     2881 cggtgcaaat aggggaagtt acttctgacc acatcctgag gggtcaagcg agaacaactt
     2941 gggcaaagaa ggttttgatg gcaagtgcca ggccaggcac aagtcgcgcg gtgcaactgg
     3001 ctacaccagc ggtttgcaac aacgcatggc gcttgtcgat gcgaccgaac tgttcaagtc
     3061 attttttgcc tgatacctca gggcttgctg ctccggtatt ggcgcaccag tgccttgact
     3121 tcgggggaat caggctgctt gcgttgcagc gcctcaaggt agggggcata gagcgcggga
     3181 tcctggcgtt cgcgcaccag gccgagcatg gacagctcgg gcaacaggtc atctggtgcg
     3241 gctgccgccg tccagaccag gtagtcggcg tgcgccttgg caatcgtcgc gttgtccgga
     3301 ttcagatgca cgccgcgcag cgtcaggcta cccaggcgct gaacgttacc cgcaaaggcc
     3361 aagctgtcgc gacttgggac acccttgacc tcgctgacgg tggcgacatg accggtgacg
     3421 cgggtgccgc ctgccgtgcc cgcgcgaaag ctgcttgggc cttgccagag ttcgcgcttg
     3481 cgggcatcaa aataaacaat ttgcgccagt ccaccagcgg ccaccgtcac caggtcgccg
     3541 tcacgcacct tcatgaaggc cagggcgcga aaactgctgc cgctgctgct ttgcaccgac
     3601 acctcgccac tcaaacgatc aaaaatgccg acatcaccgg ccgcctgcgc cgccagcgca
     3661 tggagcaaaa caacagttgt caacaagact tggaaaaatc gcaacatttt caactccggt
     3721 ttcaggtttc acgtgtcagg tttcaggttt caggtctcag gatggtacgg tatttcaaac
     3781 acctcaatgg cgctggcttt gcctttcagc gataccgact gactggcccc cagccgcagg
     3841 ccgctgccag ccgcctgcac cgtggcggcg ctggccacga tgcgccagcc cagggttttg
     3901 ctggccgact ccagccggga ggccacattg acggtatcgc caatggcggt gaactcaatt
     3961 cgcccggccg tgccgaggtt gcccacgacc accggaccgc tgtgcagccc gatgccaatg
     4021 gcaaacggtg gcagcttgcg atcagcaaag cgggtcgcca cccagacggc gaattcgtcg
     4081 gctgccagcg caacggtgcg cgctgcgtca attgcacgtt gggcatgatc tggcaacggt
     4141 aacggcgagc caaacactgc catcacggca tcgccaatga acttgtccac attgccgtgc
     4201 gccgccagaa tcggatcgca ggcctgcggc agccaggcgt tgagcagttc gaacacctcg
     4261 cggctgctga gcttttcgga aatcgaggtg aaatcgcgga tgtccagaaa cagcaccgtc
     4321 acatcagccg cagcgccatc ggtatcgggc agcgcatcgc gcaccgccag ttcggccact
     4381 tcgtcagaaa catagcggga aaacagccgg cgcaggtggg cgcgctcgcg tgcctcgcgc
     4441 ccggcccggt tacccagccc gaccgtccag gcactggcca gccccaacag caggggagtc
     4501 ggcgcgagcc agacatcatg cccaaacaag gccgcactga gcagcaggct ggccactgcc
     4561 agcagcgcgg caaggctggc cagcaggatc ggcgtgctgc gcagcgccac tgcccccagc
     4621 agcagcccca gcgctgccgc cagcagcggg ccttgccagg cagcgggcac ggctatcgtg
     4681 cgctgcccca gccaggcctc aaccacctgg gcatgcactt cgccaccgct catcaactgg
     4741 ggtggcagtc ccggcagcgc ccgcgcgtaa ggggtcaact gaaggtcctg cagttgggtg
     4801 gcatcggcgg caatgatgac gatccggtcg cgcagcgcct gaacttgcgg ctgtttcagg
     4861 gcatcggggt cggtcagcgt ggccagggaa acccggggca cggtgccggg tggaccggca
     4921 aatggaatgg tcagggccgc gctggcaaat tgccgctctg gctgcgccgc cagtaccagg
     4981 cgtgccgcaa acgccagtga cggcgagttg ctggctaaca ccggtgccag tcgccgcaca
     5041 aagccgtcgc catcggcctc aagcgtgacc aggccaatgt cgtcagcccc gccgggcagc
     5101 aaggcaatgt gctcggcggg cggcatcagg tcggcttcgc cgggttgtcc ggcacgggca
     5161 ccagccagca ccacctggcc atcggccagt gcggcgcggt agggcgcttc atagctgcga
     5221 ccagccggtg tatcgccagc acccaaaacg ttcagccagg cttcggcgct gctggcctga
     5281 atcaggtcaa tcccgatcac cttgaccccg gcctggcgca aggtggcaat ggcggcacca
     5341 aactgtggct gccagaaggc cagcggggta tcgtggaagc gcgccaggct ggtgtcatca
     5401 atggccacaa tggcggtgtg cattgcttcg gtgcgctgtc cggccagccg gtgccaggca
     5461 tcaaacacaa acaggtccat ggctgccatc cagggcaaca agggcgcaat cagtgtcgcc
     5521 agcgcggcgg tcagtgatgc gctcgccaca gcgagccatc ggtggcgcaa ggcaggcatg
     5581 gtggtttcct tgattcgggg tagatggcgt ggttcagaag cgggtaacac tttaaccttg
     5641 tttcagcact gttcggtggc atcgacaagg ggcgagttcc agagtcacgg atatttgctg
     5701 tgcttcatca actgcagccc atcggcaggt tcacatcgac ttttttcggc cgccagactc
     5761 ccttggcagt aacgcattgc actggccatt tgtgcaacgg ccgatgctgc cgacatcggc
     5821 caatttgcca cggagaatga ggtcagccgc agacccgtca agcagccaac gccctgatgt
     5881 gggccgctac ccaaacggcg gatgacgggg gtacggcttt cttccgcgct tgccaaagag
     5941 tggttgcgtc aggatcccag gtcgcccttt ctctcaatct catctgtcat caccgctgcc
     6001 ttggtgcgct ggattgccgc gctgcagtgc tgtcattgaa cggacacact ggcctgtcat
     6061 gattcagatt aagccgttgt tcatttgcag catcgctttg atcgttggga gtcatcaaac
     6121 aatatttatc ttgaagcggg tccatatgaa tattcgtcaa ctgatcgttt tgcttattgt
     6181 cctggccgtt ggtggcatgg gcttcggggg cgggatcgcc gccttcatgt cgcgtggcag
     6241 cgcttcagaa gtcaagactg tgaccgaagg cgttgtgccc agtgcgctgg catcggttga
     6301 attgatgagc cagctcaagg acgtgcagat cgccacgctg gcgatggtgt cagcacctga
     6361 tgctgatgca gttgccaaag cccagcaaga cctctctggc cgaaaagcgc tgctgcaaga
     6421 ggcattgcgc ctgcagagtg tgcaggcaga cagcaatgcc cagcgggggc tgatcacaca
     6481 ggcccaggaa agcctggtca actatttcgc gtcgattgat gacaccgcca agttcagaat
     6541 tgccgggcaa aaagagatgg ctgaggcgtt tctgggtgcc acggtagacc agtacttgcg
     6601 cgaacagatg tccatcatcg aaaccataca gatcgaaaag cgccgcagca aggacgaagc
     6661 cattcttcgg atgaatgaca atttgcgtgc ttccacgacg acgctcctga tcattaccct
     6721 ggtgtctgtg gcgatgatgg gcatggtggg gttgctgctg taccgccgaa tcatccatcc
     6781 gatcaacgac atgcagtcga agatgacaga tatcgccacc agtcaggact ttagccaccg
     6841 ggttccggtc aagcgatccg acgaaattgg aagatccatc acggccttca acaccatgat
     6901 cgagaaaatt caggaaagct ccgaactggt caagcaaaag accgccgata tccaggcgat
     6961 gttgcactac ataccgcagg gcattttgac agtgatgcct ggcaacaagg tgcatcccga
     7021 gtactcgtct tacctcgaaa caattcttga aacccgcgag atcggtggcc aggatgggtt
     7081 cttcgtcgtt ttgtgtggaa gttgcattga aaaataaaat gtagtagt
//
LOCUS       DAOSLL010000175         7108 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9608_length_7108_cov_4.960868, whole genome shotgun sequence.
ACCESSION   DAOSLL010000175 DAOSLL010000000
VERSION     DAOSLL010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7108)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7108
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9608_length_7108_cov_4.960868"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="PLB25_09235"
     CDS             <1..189
                     /locus_tag="PLB25_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HOE41796.1"
                     /translation="YPHALVRLHFCKVFDWTGELQMLEGQAFAWQTLPVTVTPVLPGT
                     LPVLHWLAAERGFSGTII"
     gene            complement(266..475)
                     /locus_tag="PLB25_09240"
     CDS             complement(266..475)
                     /locus_tag="PLB25_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HOE41797.1"
                     /translation="MTGATPSERLIARSVACPTCGGPSRYAPDNPFRPFCCERCKQID
                     LGAWASEQFAVAAPLLPDDPLSNLN"
     gene            complement(472..1227)
                     /gene="zapD"
                     /locus_tag="PLB25_09245"
     CDS             complement(472..1227)
                     /gene="zapD"
                     /locus_tag="PLB25_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HOE41798.1"
                     /translation="MILYEYPLHERIRTYLRLEHLFLRLHQLAARIDDLDHHFALVTL
                     FEIMDVTARADLKSDLLKDLDKQKHVLEAYRGNPAIVEAALEKVIGELEQSFDALNNQ
                     PGKAGQTLADNDWLMSIRSRISIPGGTCEFDLPSYYAWQHKTGVSRQADLTQWIATLA
                     SVGDAVHLLLKLLRDTGAPQKIMANGGQFQLTLPQGRTFLLLRLALEVSQELIPEISG
                     NRLMVSVRLMRLGADQRPHPVTEDVAFELALCS"
     gene            complement(1229..1864)
                     /gene="coaE"
                     /locus_tag="PLB25_09250"
     CDS             complement(1229..1864)
                     /gene="coaE"
                     /locus_tag="PLB25_09250"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481802.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HOE41799.1"
                     /translation="MPAFKLGLTGGIGSGKSTVAAMLAGLGAAVMDADAISRAVTAPG
                     GAAIGAISQAFGAGFITPEGALDRSRMRSLAYTDASARQRLEAIIHPLVNQEIWQQAR
                     LAEAAACPCLVFDVPLLIESPRWRQQVDQVLVVDCSPETQITRAMARSALDRPAVEAI
                     IAAQASREQRLMAADLVILNDHISLAELAQHVRTLAAGFGLSLHHIQPPAH"
     gene            complement(1871..2713)
                     /locus_tag="PLB25_09255"
     CDS             complement(1871..2713)
                     /locus_tag="PLB25_09255"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HOE41800.1"
                     /translation="MIMSQGLDASLLGLLGLLIGSFLNVVIYRLPVMMEAHWKAECAE
                     LAGQAPVAGTAFNLMTPRSRCPHCGHPIRWFENIPLISFLMLRGKCSHCQTAISVRYP
                     AVELATGALFFFCAWRWGLTPVALAWCGFAAALLTLALIDWDTTLLPDDITLPLLWAG
                     LVAAALGLSHVPLADSLWGAVGGYLSLWLVYWGFKLATGKEGMGFGDFKLFAALGAWF
                     GWQALIPIILMSSVIGALIGIAMKFSSGLREGGYIPFGPFLAGAGLTAMVVGPTAILK
                     VVGL"
     gene            complement(2713..3930)
                     /locus_tag="PLB25_09260"
     CDS             complement(2713..3930)
                     /locus_tag="PLB25_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOE41801.1"
                     /translation="MATDKSKAIKESVFEWEGKDRNGKQVRGETRAAGENQVKAALRR
                     QGVMPSKIKKRRMSAGRSIKPKDMAIFTRQLATMMKAGVPLLQAFDIVGRGNPNPSVT
                     RLLNDIRTDVETGTSLSVAFRKYPLYFDNLYCNLVEAGEAAGILDQLLDRLAVYMEKT
                     EAMKSKIKSALMYPIAVLIVAFVVTAVIMIFVVPSFKEVFTSFGADLPAPTLAVIAMS
                     EIFVKWWWLIFGGLGGGLYFFMQAWQRNKQVQIFMDRLMLKLPIFGILVEKSCIARWT
                     RTLSTMFAAGVPLVEALDSVGGAAGNWVFESGTIKIQQEVSTGTALTTAMTNANLFPS
                     MVLQMCAIGEESGSIDHMLGKAADFYESEVDDMVAGISSLMEPIIIVILGTVIGGIVV
                     AMYLPIFKLGQVV"
     gene            complement(4002..5735)
                     /gene="pilB"
                     /locus_tag="PLB25_09265"
     CDS             complement(4002..5735)
                     /gene="pilB"
                     /locus_tag="PLB25_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HOE41802.1"
                     /translation="MAAIETNLPPSSASALPGLGRALVMAGKLDQRFAEEIYRKALAK
                     RSSFIAELTGSGALTAADLAHTLSLAFAVPLIDLDAVDVQQLPRGLLDNKICQDYRLV
                     VLGKRQNRLIVATADPSDQQSVEKIKFSTQQNVDWVIAEYDKLLKMVSAISATVAESI
                     NSIVGNDFEFDDMATDSVSESANASAAEVDDAPVVKFLQKMLVDAFTMRASDLHFEPY
                     EHTYRVRFRIDGELREIASPPIAIKDKLASRIKVISKMDISEKRVPQDGKMKLKIGPD
                     RVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIDALGYEPVEKDRILQAITRPYGMILV
                     TGPTGSGKTVSLYTCLNLLNKPGVNIATVEDPSEINLPGVNQVNVNEKAGLTFAAALR
                     SFLRQDPDIIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPSTLTRMRNMGIAPF
                     NIASSVLLISAQRLARRLCPSCKTAIDIPQKALLEAGFKASDLTGNWKPYHPVGCTAC
                     NNGYKGRVGLYQVMPISEEIQRIILRDASALEIGEQARFEGVNTLREAGLVKVKLGLT
                     SLEEVLAVTNE"
     gene            complement(6066..6215)
                     /locus_tag="PLB25_09270"
     CDS             complement(6066..6215)
                     /locus_tag="PLB25_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41803.1"
                     /translation="MPGSDGIQAARHGTAVQQQTAALSLATLPLPHPTLMALPYLAAT
                     LIDRD"
     gene            complement(6215..6895)
                     /locus_tag="PLB25_09275"
     CDS             complement(6215..6895)
                     /locus_tag="PLB25_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006463578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOE41804.1"
                     /translation="MNTLLDHVQIESAPNPSAAVIWLHGLGADGHDFAPLVPELDLRH
                     CPPIRFIFPHAPSIPVTVNGGFVMPAWYDIRGTDLTSRQDDVGIVASESAICALIERE
                     IARGIPAGRIVLAGFSQGCAIALHTGLRYASALAGIVALSGYLPLADRLVHERNPANA
                     STPIFMGHGSQDPVVPQARGEASRDLLVKLGYQVQWHSYPMPHSVHPREVADIAAFLT
                     RVLGRPAS"
BASE COUNT         1442 a         2094 c         2125 g         1447 t
ORIGIN      
        1 tacccgcacg cattggtgcg tctgcatttc tgtaaagtgt ttgattggac gggtgaactg
       61 cagatgcttg agggccaggc ttttgcctgg cagacgctgc cggtgacggt aactccggtg
      121 ctgcccggca ccctgccggt gttgcattgg ctggcagccg agcgcggctt ttcaggcact
      181 attatttaga tagctgctag cgcccgtcca gcaagggctg gggcaccatt tagcttgtga
      241 aattcgcgtg aaacgcctgg ctgcatcaat tgaggttgga caaggggtca tcgggcagca
      301 acggtgccgc tacggcaaac tgctcgctgg cccaggcccc caggtcgatt tgcttgcagc
      361 gttcacagca gaacgggcga aacgggttgt cgggtgcata ccgactcggt ccgccacagg
      421 tcgggcatgc gaccgatctg gctatcagcc tttcagacgg cgttgcccct gtcatgaaca
      481 caaggccaac tcgaacgcta catcttcagt aaccgggtgg ggtcgttgat cagcaccgag
      541 gcgcatcagc cttaccgata ccatcaagcg gttgccgctg atttctggaa tcagctcctg
      601 cgacacctca agtgccaggc gcagtaataa aaatgtgcgt ccttgtggca aagtcaactg
      661 aaactgcccg ccattggcca tgattttttg cggcgcacca gtgtcgcgca gcagtttgag
      721 cagcaggtgg acagcatcgc caaccgatgc gagcgtcgcg atccactggg tcaggtcagc
      781 ttgtcggctg acaccggttt tgtgttgcca ggcgtagtaa gaaggcaggt cgaattcgca
      841 ggtgccgcct ggaatgctga tgcggctgcg aatgctcatc agccagtcgt tgtcggccag
      901 cgtctgcccg gccttgccag gctgattgtt cagcgcatcg aaactttgtt caagttcgcc
      961 gatcaccttt tccagcgcgg cttcgacgat tgccgggttg ccacggtagg cttccagcac
     1021 gtgcttttgc ttgtccaggt ctttcagcag atcggatttc aggtcagccc gggccgtcac
     1081 gtccatgatc tcaaacaggg tgaccagggc aaagtggtgg tcgaggtcgt cgattcttgc
     1141 ggccaactgg tgcaggcgca aaaacaggtg ttcgaggcgc aggtaggtgc ggatgcgttc
     1201 gtgcagggga tattcgtaaa ggatcacgct aatgtgctgg gggctgaatg tgatgcaatg
     1261 atagcccgaa gccagcggcc agggtgcgaa catgctgcgc cagctcagca agggaaatat
     1321 ggtcattcaa aatgaccagg tctgcggcca tcaggcgttg ttcgcggctg gcttgcgccg
     1381 cgatgatggc ttcaaccgcc gggcgatcca gggcactgcg cgccatcgcc cgtgtgattt
     1441 gggtctcggg gctgcagtcc accaccagca cctgatcaac ttgctggcgc caccggggtg
     1501 actctatcag caagggcaca tcaaacacca gacaggggca ggcagccgct tcggccagcc
     1561 tggcttgctg ccagatttcc tggttgacca acgggtggat gatggcctcc aggcgctgcc
     1621 tggcactggc atcggtgtac gccaggctgc gcatgcggct gcggtccagt gccccctcgg
     1681 gggtgatgaa tccagcacca aaagcctggc ttattgctcc aatcgctgcc ccgccagggg
     1741 cggtgactgc acgcgaaatg gcatcggcat ccatcacagc ggcccccaac cctgccagca
     1801 tcgcagccac ggtacttttg ccactgccga tgccgccggt tagcccgagc ttgaacgctg
     1861 gcaccaccgt ctaaagccct acaaccttca agatggcggt cggcccaacc accatcgccg
     1921 tcagaccggc accggccagg aagggcccga acggaatgta gccgccctcg cgcaaaccgc
     1981 tggaaaattt catggcgatg cctatcaagg caccaatcac cgaggacatc aggatgatcg
     2041 gaatcagcgc ctgccagcca aaccaggcgc ccagtgcggc aaacagcttg aagtcgccaa
     2101 aacccatgcc ctccttgccg gtggccagct tgaagcccca atagaccagc cacaaagaca
     2161 ggtagccacc gactgcaccc cagagcgaat cagccagcgg gacgtggctc aggcccagcg
     2221 ccgcggcgac cagtccggcc cacagcaacg gcagcgtgat gtcgtcgggc agcaacgtag
     2281 tgtcccagtc aatcagcgcc agtgtcagca aggccgccgc aaagccgcac caggccagcg
     2341 ccaccggtgt aagcccccag cgccaggcgc aaaagaaaaa cagcgcgcca gtggccagtt
     2401 caaccgctgg gtaacgcaca ctgatggcgg tctgacaatg gctgcatttg ccccgcagca
     2461 tcaggaagct gatcagagga atgttctcaa accagcgtat cgggtggccg cagtgcgggc
     2521 agcgcgagcg gggtgtcatc aaattgaagg ctgtcccggc cactggcgct tgtccggcca
     2581 gctcggcgca ttctgctttc cagtgcgcct ccatcatgac cggcaggcgg taaatcacca
     2641 cattcagaaa gctgccaatc agcagcccca gcaagcccag caagctggca tcgagccctt
     2701 gcgacatgat catcaaacga cctgaccaag cttgaagatt ggcaggtaca tggcaaccac
     2761 aatgccgcca atcactgttc ccaggatgac gatgatgatg ggctccatca ggctggaaat
     2821 gccggccacc atgtcgtcca cctcggactc gtaaaagtcg gcggccttgc ccagcatatg
     2881 gtcaatcgag ccggattctt cgccaatggc acacatttgc agcaccatcg acggaaacag
     2941 gttggcattg gtcatggctg tcgtcagtgc ggtgccggtc gagacctctt gctggatctt
     3001 gatggtgcct gactcaaaaa cccagttgcc ggcggcacca ccgaccgaat cgagtgcctc
     3061 caccaggggc acaccggcgg caaacatggt tgacagcgtg cgggtccagc gggcaatgca
     3121 ggatttttcc accagaatgc caaaaatcgg cagcttcagc atcagccggt ccatgaatat
     3181 ctgtacctgc ttgttacgct gccaggcctg catgaaaaag tacaagccgc cgcccaggcc
     3241 gccaaaaatc agccaccacc atttgacaaa aatctcactc atggcgatca ccgccagagt
     3301 tggcgccggc aagtcggccc caaagctggt gaacacctcc ttgaacgaag gcacgacaaa
     3361 aatcatgatc acggcagtta ccacaaatgc cacgatcagc accgcaatcg gatacatcag
     3421 cgccgacttg atcttggact tcatggcctc ggttttttcc atgtacaccg ccagacggtc
     3481 gagcaactgg tccagaatac cggcggcttc accggcttcc accagattgc agtacaggtt
     3541 gtcaaagtac agcgggtact tgcgaaacgc caccgacagc gaggtgccgg tttcgacatc
     3601 agtgcggatg tcgttgagca accgtgtcac actggggttg gggttgccac ggccgacaat
     3661 gtcaaacgct tgcagcagtg gcacaccggc cttcatcatg gtggcgagct ggcgcgtgaa
     3721 gatcgccatg tccttgggtt tgattgatcg cccggcactc atgcggcgct ttttgatctt
     3781 gcttggcatg acaccctggc ggcgcagggc ggctttcacc tgattttcgc cagcggcccg
     3841 ggtctcgcca cgcacctgtt tgccgttgcg gtccttgcct tcccactcaa agactgattc
     3901 tttgattgct tttgacttgt cggttgccat gttgtgctgt tgttgttgct gctggtgctg
     3961 tggttggttg aaaggctggc caggtgcgat tgatgcgcgg cctattcatt ggtgacggcc
     4021 agaacttctt ccagcgaagt aagtcccaat tttactttta ccaggccggc ttcgcgcagg
     4081 gtgttgacac cctcgaatct ggcctgttcg ccaatctcaa gcgcgcttgc gtcgcgcaag
     4141 ataatgcgct gaatttcctc gctgatgggc atcacctggt acaggccgac gcggcccttg
     4201 tagccattgt tacaagcggt gcagccaacc ggatgatatg gcttccagtt gccagtcagg
     4261 tcgctggctt tgaatccggc ctcaagcagg gctttctgcg ggatgtcaat ggccgttttg
     4321 caactggggc acagccgccg ggccaggcgc tgtgccgata tcaacagcac acttgacgca
     4381 atattgaacg gtgcaatccc catgttgcgc atccgggtga gtgtcgatgg tgcgtcattg
     4441 gtgtgcaagg ttgacagcac cagatgcccg gtttgtgccg ccttgatggc gatatcagcg
     4501 gtttccaggt cgcggatttc acccaccatg atgatgtccg ggtcttgccg caaaaacgag
     4561 cgcagcgcag cggcaaaggt caggccagcc ttttcgttga cattgacctg gttgactccg
     4621 ggcaggttga tttcggacgg gtcttccacc gtggcgatgt tcacgccggg cttgttgagc
     4681 agattgaggc aggtgtacag cgacaccgtc ttgcccgagc cggttgggcc ggtgaccaga
     4741 atcatgccgt aagggcgcgt aatggcctgc aatatgcgat ctttttcaac cggttcatag
     4801 cccagcgcat caatgcccaa cttggcgctg ctggggtcca gaatgcggat cacgatcttt
     4861 tcgccaaaca gtgtgggcag ggtgctgacg cggaaatcga tcacccggtc cggcccaatc
     4921 ttgagcttca ttttgccgtc ttgcggcaca cgtttttcgg aaatgtccat tttcgaaatc
     4981 accttgatac gcgatgccag cttgtccttg atggcaatcg gtggcgaggc aatttcacgc
     5041 agctcaccgt caatgcgaaa acgcacccga taagtgtgct catagggttc aaagtgcagg
     5101 tccgaggccc gcatggtgaa agcgtcgacc agcatttttt gcaaaaactt gaccaccgga
     5161 gcgtcgtcaa cttcggcggc actggcattg gcgctttcgc tgacggagtc ggtcgccatg
     5221 tcatcaaatt cgaagtcatt gccgacgatg ctattgatcg actctgccac cgtcgccgaa
     5281 atggccgaca ccattttcag cagcttgtcg tattcggcaa tcacccaatc gacgttctgc
     5341 tgggtggaaa acttgatctt ctcgaccgat tgctggtctg acgggtcagc cgtggcgacg
     5401 atcaggcggt tttgccgctt gcccagcacc accagccgat agtcctggca aattttgttg
     5461 tccagcaggc cgcgcggcaa ttgctgcaca tcgactgcgt cgaggtcaat caggggcacg
     5521 gcaaacgcca gtgacagggt gtgtgccaga tcggcagccg tcagggcacc agagccggtc
     5581 agctctgcga tgaagctcga ccgctttgcc agcgccttgc ggtaaatttc ttcggcaaac
     5641 ctttgatcta gtttgcccgc catgaccagg gcgcgcccaa gaccgggcag ggcgctggca
     5701 gaagatgggg gtaagttggt ttctatggca gccataaaga gtgaatgtac tacttgaatg
     5761 caggttttgc gcatagcggg ccacgcttga cgtgcatata cccgtcagta cctgttggtt
     5821 tttccctaat gctgacagcc tgcagggtgt tggtcttgac cccgaccggt agggccgagc
     5881 cgtccgcacc cgctggtcaa tccggtcctc aatgataaat cgtgctgtag cggttgcgcc
     5941 atatgcgctg acagctacat attcagtagc tatctggcag atgaccgggc tgctgggcaa
     6001 cgctgtcgcc ctggccgcga tgcgcaggtg gccaagccgg acaaccgcag tgctgattga
     6061 ccgggtcagt cccggtcaat cagggtagcg gccaggtacg gcaacgccat caaggtcggg
     6121 tgcggcagtg gcagggttgc cagcgacagg gcggcggttt gctgctggac cgctgtgcca
     6181 tggcgcgctg cttggatgcc gtccgatcct ggcatcaact ggcggggcgg cccagcaccc
     6241 gcgtcaaaaa ggcggcgatg tcggctactt cgcgcggatg cacgctgtgc ggcatcgggt
     6301 agctgtgcca ttgcacttgg taacccagtt tgaccagcag gtcgcggctg gcctcgccgc
     6361 gtgcctgggg caccaccggg tcctggctgc catgccccat gaaaatcggc gtgctggcgt
     6421 tggccgggtt gcgctcgtgt accaggcgat cagccagcgg caggtaaccc gacagcgcca
     6481 cgatgccagc cagcgccgac gcataacgca aaccggtgtg caaggcaatc gcgcagcctt
     6541 gcgagaaacc ggccagcacg atccgcccag ccggtatgcc acgcgcgatt tcccgctcga
     6601 tcagggcgca aatggcggac tcggacgcga caatgccgac atcgtcttgc cgactggtca
     6661 ggtcggtgcc ccgaatgtca taccaggccg gcatcacaaa accgccgttg acggtgaccg
     6721 gtatgctggg tgcgtgcgga aagataaagc ggattggcgg acagtggcgc aggtcaagtt
     6781 ccggcaccag gggggcaaag tcatggccat ccgcgccgag tccgtgcaac cagatgaccg
     6841 cagcactggg gttgggggca gattcaatct ggacatggtc aagcaaggtg ttcatgggtg
     6901 ttctttcaaa gtgaggcctt attgttcgac gctgcagcac cggtcgccct cgatctggac
     6961 cacctgactg ccttggggtg caggcaaccc aaaggcgaca ggcccgacct ggctgcgttg
     7021 caacggcgct gcgggcattt ggtcaggaca gcaaggcctg ggcaaattcg cgggcgttga
     7081 aggtttccag gtcttcaagc ttttcgcc
//
LOCUS       DAOSLL010000176         7096 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9630_length_7096_cov_4.966766, whole genome shotgun sequence.
ACCESSION   DAOSLL010000176 DAOSLL010000000
VERSION     DAOSLL010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7096)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7096)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7096
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9630_length_7096_cov_4.966766"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PLB25_09280"
     CDS             <1..288
                     /locus_tag="PLB25_09280"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOE41805.1"
                     /translation="TMIVAELVLGVLASIIVMWFSRQHEFRADAGGATLAGRQNMIAA
                     LQRLMQAHTPPLPEKMAAFGISGGGGSGLKRLFMTHPPLEERITALQNAQL"
     gene            549..1178
                     /locus_tag="PLB25_09285"
     CDS             549..1178
                     /locus_tag="PLB25_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOE41806.1"
                     /translation="MELSTWLTFFAASWAISISPGPGAVASMSAGLNHGFRYGYVIIF
                     GLVLGIWTQLAVVGVGLGALMATSSMAFTVVKWLGVGYLVWLGIAQWRAPARPMVAAS
                     DAGEVVTQRSLILKAWMINVVNPKGTVFLLAVVPQFISLTEPLLPQYLIIGATLAFTD
                     LVVMTGYTALAAKVLGALKEPAHIRAMNRVFGSLFVLAGSLLALFKRSV"
     gene            complement(1428..1871)
                     /gene="ybeY"
                     /locus_tag="PLB25_09290"
     CDS             complement(1428..1871)
                     /gene="ybeY"
                     /locus_tag="PLB25_09290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HOE41807.1"
                     /translation="MKQLRLSLQFGKLADAAQHRAALPRHMVNRWIRHALQDDAEITV
                     RIVGTEEGQALNRDYRHKDYATNVLTFDYTQEPLVTADLVLCAPVVAKEAAEQGKTLQ
                     AHYAHLLIHGTLHAQGWDHDEDEDAQVMELRESEIMARLGFDNPY"
     gene            complement(1945..2940)
                     /locus_tag="PLB25_09295"
     CDS             complement(1945..2940)
                     /locus_tag="PLB25_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOE41808.1"
                     /translation="MILRHIFSPPSNTRLAHLCGPMDVHLRSLEAALQVRIAHRHEQF
                     KVDGSKANARRGMDMLQALYELAARPIEPRTVQLMLSGDGDVNGTDGPSLKTRRADLK
                     PRSINQATYLDNIASHDITFGIGPAGTGKTYLAVAMAVDALQRSTVQRIVLTRPAVEA
                     GERLGYLPGDLAQKIDPYLRPLYDALYDLMGFEAVQKAFERQTIEIAPLAFMRGRTLN
                     TAFVILDEAQNTTPEQMKMFLTRIGFGSKAVVTGDVSQIDLPANQFSGLIDAERVLKS
                     IEGIAICRLTSADVVRHPLVARIVDAYDAPGRFNPHPRDDAQAAPARTRLRKLAT"
     gene            2981..3157
                     /locus_tag="PLB25_09300"
                     /pseudo
     CDS             2981..3157
                     /locus_tag="PLB25_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold domain-containing protein"
     gene            complement(3238..3756)
                     /locus_tag="PLB25_09305"
     CDS             complement(3238..3756)
                     /locus_tag="PLB25_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOE41809.1"
                     /translation="MANLGRVIEADELRLVAGNISEWARRIRELRTEEGYLILTNHDR
                     ADLKPGEYVLETAKPQPAFARGISKETRAFVLDRNGFTCQMCGAVAGEPHPFDATRKT
                     RLHIGHVIDKSLGGSDEANNLKAICSVCNEGAANITLQRPDLNKLLVQVRRATAADQR
                     ELLKWLRQKFKE"
     gene            complement(3825..4784)
                     /locus_tag="PLB25_09310"
     CDS             complement(3825..4784)
                     /locus_tag="PLB25_09310"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HOE41810.1"
                     /translation="MNSLELCAGAGGQAQGLERAGFQHEALVEIDTPACNTLRLNRPH
                     WNVIEGDLTEFDARPFRGVDLVAGGVPCPPFSKAGKQLGEGDARDMFPHAIRVVDETR
                     PQAVMLENVRGLFDDVFVDYRQKISRQLRALGYWTDWHLFNAADFGVSQLRPRVVFVA
                     LQCDVAEHFRWPAPSLKLPPTVGQLLHDLMHMHGWRGADAWRDLANDIAPTLVGGSKK
                     HGGPDLGPTRSRQAWATLGVDGRGLADDAPAADFVGIPRLTVRMCARIQGFPDDWQFV
                     GKKTPIYRQVGNAFPPPVAHAVSAEIAAAIEKAAAVRVHRRAA"
     gene            4997..5569
                     /gene="ruvA"
                     /locus_tag="PLB25_09315"
     CDS             4997..5569
                     /gene="ruvA"
                     /locus_tag="PLB25_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HOE41811.1"
                     /translation="MIGKLTGTLDHKNPPEVIIDCHGVGYEVLVPMSTFYNLPELGTK
                     VSLLTHFVVREDAQILYGFASAAERAAFRELIKISGVGPRTALSVLSGMSVAELAQAI
                     TLQEAGRLVKVPGIGKKTAERLLLELKGKLGPDIGVVATVASDAQTDILQALLALGYS
                     DREAAAALKALPNDVGVSDGIKLALKALAK"
     gene            5788..6339
                     /locus_tag="PLB25_09320"
     CDS             5788..6339
                     /locus_tag="PLB25_09320"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HOE41812.1"
                     /translation="MATPIKLQRLTPACAATYRGLMLEAYALQPQAFTSSVAERSALP
                     LSWWQARLQITETAGEAVFGAWDGEVLAGVVGLAFESREKTRHKATLFGLYVRAPYRQ
                     RGLGRQLLCSAWNFLGSRPEVKVVQLTVTTGNSAAQSLYQQFGFVAFGTEPMAVAVAG
                     AYVSKVHMWCSVESSSPIRHGGG"
     gene            6410..>7096
                     /locus_tag="PLB25_09325"
     CDS             6410..>7096
                     /locus_tag="PLB25_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE41813.1"
                     /translation="MKAICTKALRLTVTAAAALTLASGSALAADKVKVGMLSTLSGPG
                     AALGIDIRDGFNLALKHSGGKFGGLPVEVIVADDQASPDAAKQTADRLVKKDKVDFMT
                     GVVFSNVMLAVGKPIFDSKTFYISANAGPSQYAGAQCNPYFFSASYQNDNLHEAVGKT
                     VQDKGFKKVALLAPNYPAGKDALTGFKRFFKGTIVSEEYTPLSQLDYGTELSKMRASG
                     ADAVYIFLPGG"
BASE COUNT         1365 a         2025 c         2153 g         1553 t
ORIGIN      
        1 accatgatcg tcgccgaact agtgcttggc gtcctcgcct cgatcatcgt catgtggttc
       61 tcgcgccagc acgaatttcg cgccgatgcc ggcggcgcca cattggcggg tcggcagaac
      121 atgattgccg cactgcaacg cctgatgcag gcgcataccc cgccgctgcc tgaaaaaatg
      181 gccgccttcg gcatcagcgg tggcggcggt agcggtctca agcgcctgtt catgacgcac
      241 ccgccgctgg aagaacgcat tacggcgctg caaaacgcgc agttgtaggg tgcgcaaagg
      301 cagcgcctgc cagcaaaacg ctttcgagcg tcccgacccc gctcgcgggc cgggcgttct
      361 caagggaaca tggggttgag gccgccatcg caggcgctgc atcccaccca gtttttatct
      421 gccaaatcgg gttacatccc tgatgtattg tgcgttgcta gctactgttt tgattgctaa
      481 cagaggattt gttcctggtg agtcaacagc gcatgccagg tagccggtgg gtcggctaga
      541 cttgaaccat ggaactctct acctggttga cttttttcgc ggcctcctgg gccatcagca
      601 tttcgcctgg cccgggtgcc gttgcttcga tgagcgcggg gttgaaccac ggcttcagat
      661 acggctacgt catcattttc gggctggtgc tgggcatctg gacgcaactg gctgtggtcg
      721 gggtgggctt gggtgcgctg atggccacct ccagcatggc gtttaccgtg gtcaagtggc
      781 tcggtgtggg ttatctggtg tggctgggca tcgcgcaatg gcgggcaccg gcgcggccga
      841 tggtggcggc atcggacgca ggcgaagtcg tgacccagcg ctcgctcatt ttaaaggcct
      901 ggatgatcaa cgtggtcaac cccaagggca cggtgttttt gctggcggtg gtgccgcagt
      961 tcatcagcct gaccgagccg ctgctgccgc agtacctgat catcggtgcc acgctggcgt
     1021 ttaccgacct ggtggtgatg accggctata ccgcgctggc ggccaaagtg ctgggcgcct
     1081 tgaaggaacc cgcgcacatc cgcgccatga accgcgtctt tggcagcctg tttgtgctcg
     1141 ctggttcgct gttggcactg ttcaagcgct cggtctgagc ggcgggcagc ctgcgcccgg
     1201 gctggcgatg ccagggagca gcgccagacg ttcactggcc aaatctgctg tgtcacggtg
     1261 aaatcgcact tgcgatgcca tccggccatc aaacagcaga tgttggtgct tgcggctggg
     1321 tagcagcgca ggtcaatccg gccacgcatg cgcggctttg cagcgcgata agcagttgtc
     1381 aactcaatga ggaattcagc gacgcgcacg gctttgcgcc agtgctgtca atacgggttg
     1441 tcaaacccca gccgcgccat gatctcgctt tcgcgcagtt ccatcacctg ggcgtcttca
     1501 tcctcatcat ggtcccagcc ctgtgcgtgc agggtgccgt gaatcagcag gtgggcgtaa
     1561 tgcgcttgca gcgttttgcc ctgttcggcg gcttctttgg ccaccaccgg ggcgcacagc
     1621 accaggtcgg cagtcaccag cggctcttgc gtgtaatcaa aggtcagcac gttggtagcg
     1681 tagtctttat ggcggtagtc gcggtttaac gcctggcctt cttctgtacc cacaatgcga
     1741 acggtgattt cagcatcatc ttgcagcgcg tggcgaatcc agcgattgac catgtggcgg
     1801 ggcagggcgg cgcggtgttg cgctgcgtcg gccagtttgc caaattgcag ggaaagcctc
     1861 agttgcttca taagtttgta agaaataggg ctcaagccct tatggaatat gcgctgatag
     1921 ctcttaataa cagagcatct tgaatcaggt ggcgagcttg cgcaagcgtg tgcgggcggg
     1981 cgcggcctgg gcatcgtcac gcggatgcgg attaaaccgg cctggcgcgt cgtaggcatc
     2041 aacgatgcgg gccaccagcg gatggcgcac cacgtcagca ctggtcaggc ggcaaatcgc
     2101 aatgccctca atgcttttga gcacccgctc ggcatcaatc aggccgctga actggttggc
     2161 gggcagatcg atctggctca cgtcaccagt caccactgcc ttggagccaa agccgatgcg
     2221 ggtcaaaaac atcttcatct gctccggcgt ggtgttttgg gcttcatcca gaatcacaaa
     2281 cgcggtgttg agggtgcgtc cacgcatgaa ggccagtggc gcgatctcga tggtctggcg
     2341 ctcaaaggct ttttgcaccg cctcaaagcc catcaggtcg tacagcgcgt cgtacagcgg
     2401 gcgcaggtag gggtcgattt tttgcgccag atcacccggc agatacccca gccgttcccc
     2461 ggcttcgact gccgggcgcg tcagcacaat gcgctgcaca gtgctgcgct gcagcgcatc
     2521 gaccgccatg gcgacggcca ggtaggtttt gccggtgccg gccgggccga tgccaaaggt
     2581 gatgtcatgg ctggcgatgt tgtccaggta ggtggcctgg ttgatcgagc gcggcttgag
     2641 gtcggcgcgt ctggttttga gcgagggacc atcggtgcca ttgacatcgc catcgccaga
     2701 tagcatcaac tgcacggtgc gtggctcaat cgggcgtgcg gccagctcat acagcgcctg
     2761 cagcatgtcc atgccgcgcc gggcgttggc cttgctgcca tcgaccttga actgctcgtg
     2821 gcggtgcgct attcggacct gcaatgcggc ctccaggctg cgcaaatgca cgtccatcgg
     2881 gccgcacagg tgcgccagcc gggtgttgct gggcggagaa aaaatgtgtc gaaggatcac
     2941 gttgataatt ctttgaaatg ttattgatga taggtacaaa atgatcggca aattgacagg
     3001 cacgcttgac cacaaaaatc cgcccgaagt catcatcgac tgtcacggcg ttggctacga
     3061 ggtattggtg ccgatgagca cgttttacaa cctgcccgaa gcaggcggca cagtcagcct
     3121 gctgacgcat tttgtggtgc gcgaggacgc gcagatttgg taatcggcgt gcttgccact
     3181 gtgattcacg ctggcgcaca acgcgctacc gagatcgact tcaacttggc gagtcactca
     3241 ttctttgaac ttctgcctga gccatttgag tagttcacgt tgatctgctg ccgtggcccg
     3301 gcgtacctgc accagcagct tgttgaggtc tggacgctgg agggtgatgt tggcagcacc
     3361 ctcgttacag acagagcaaa tggccttcaa attgttggct tcgtcagagc cgcccaggct
     3421 tttgtcgatc acatggccaa tatgcaggcg tgttttgcgt gtagcgtcaa atgggtgtgg
     3481 ctcacctgca accgcgccgc acatttggca agtgaagccg ttacggtcca acacaaacgc
     3541 gcgtgtttcc ttggagatgc cccgggcaaa agcaggctgg ggtttggcgg tttcaagaac
     3601 gtattcgcct ggcttcaggt cagcacggtc atggtttgtc aggatcagat aaccttcttc
     3661 ggtgcgcagt tcccggatac gccgcgccca ctcgctgata ttgcctgcaa cgagacgcag
     3721 ttcgtcggcc tcaatcaccc ggcccaaatt ggccaaaaaa tgcgcgcgca gtttcgcgcg
     3781 agcgcctttt ttcctgggtt ccaattgctt tacctgcgtt gccatcatgc ggcccttcta
     3841 tgaactcgaa ctgcggcggc tttttcgatg gccgcagcga tttctgcact gacagcatgc
     3901 gccaccggcg gcgggaaagc gttacccacc tgtcgataga tgggtgtctt ttttccaaca
     3961 aactgccagt cgtcaggaaa gccctggatg cgagcgcaca tgcgtactgt caatctgggg
     4021 attccgacaa agtctgctgc gggcgcatcg tctgccaggc ctcgcccatc cacgcccaat
     4081 gttgcccagg cctggcgcga gcgtgtcggc ccgagatccg ggccgccatg ttttttgctg
     4141 ccacccacca atgtgggcgc aatgtcattg gccagatcgc gccaggcatc tgcacctcgc
     4201 caaccatgca tgtgcatcag atcatgaaga agctggccga ccgtgggggg gagtttcagg
     4261 ctgggcgctg gccatctgaa atgttcggca acgtcgcatt gcagtgcaac aaacacgacc
     4321 ctggggcgca actgactcac gccaaagtca gccgcattga acagatgcca gtccgtccag
     4381 tagcccaacg ctcgcagttg tctggatatc ttttgccggt agtcaacaaa gacatcatcg
     4441 aacagccctc tcacgttttc aagcatgacg gcttgcggcc tggtttcgtc aaccacacga
     4501 atggcgtggg ggaacatgtc acgcgcatct ccttcaccca actgctttcc ggcttttgaa
     4561 aatggcggac acggcacacc gccagccacc agatcgacac cacgaaaggg tcgtgcgtca
     4621 aactcggtca agtcgccttc aatcacattc cagtgggggc ggttcaaacg caatgtattg
     4681 catgcaggtg tgtcaatttc aaccaaggcc tcatgctgga agccagcgcg ctccaagcct
     4741 tgggcttgac caccagcacc tgcgcagagt tctagagagt tcatcatttc gcctaaaagc
     4801 tgtatgtttt tacagtttaa ttatccactg cctagcccgg ctttggtaaa ttacttatcc
     4861 atagcagcca aatcagacgc cactcggtct gatcgccttg ttcgcagttg tttggatggg
     4921 gaaaacaggt ggcgaaggat caagttgata atgtcctgga ggaatcttta aaaaggtcac
     4981 ccatgatagg caaaaaatga tcggcaaatt gacaggcacg ctggaccaca aaaatccgcc
     5041 cgaagtcatc atcgactgtc acggcgttgg ctacgaggta ttggtgccga tgagcacttt
     5101 ttacaacctg cccgagctgg gcaccaaagt cagcctgctg acgcattttg tggtgcgcga
     5161 ggacgcgcag attttgtacg gctttgccag cgcagccgag cgtgcggcgt ttcgcgagtt
     5221 gatcaagatt tcgggggtcg ggccgcgcac cgccttgtcg gtgctgtcgg gcatgagcgt
     5281 ggccgagctg gcgcaggcca tcaccttgca ggaggccggt cggcttgtca aggtgccagg
     5341 catcggcaag aaaactgccg agcgcctgtt gctggagctc aaaggcaagc tcgggcctga
     5401 catcggtgtg gtggccacgg tggccagcga tgcccagact gacatcttgc aagcgttgct
     5461 ggcgctgggt tacagcgacc gcgaggccgc tgcggccctg aaagccttgc ccaacgatgt
     5521 gggggtgagc gatggcatca agctggccct caaagcgttg gcgaaatagc tgtgttcagg
     5581 ctttttttgt tgccattgag catcccaacc ccgcacgcgg ggcaggcgtt ctcaaaggaa
     5641 catgggctag gccgacggcg caagctgcca gggttttatc tgccaaatcg gcatccagcc
     5701 cttatgcaga ctgcgttact agctatattt ttgattgtta acataatatc tgttccttgt
     5761 gaaccttgac tgcattggta ccagcgcatg gcgacaccca tcaagctgca acgcctgacc
     5821 ccggcgtgcg ctgcgaccta ccgtggcctg atgctcgaag cctatgcgct gcagccacag
     5881 gcgttcacct ccagtgtggc agagcgctcg gccttgccgc tgagctggtg gcaagcccgt
     5941 cttcagatca cagagacggc gggcgaggcg gtgtttggcg cgtgggacgg cgaagtcctg
     6001 gcaggtgtgg tcggcctggc ctttgagtcg cgcgaaaaaa cccgccacaa agcgaccctg
     6061 tttggcctgt atgtcagggc gccataccgt caacgcgggc tgggtcgcca gttgctctgc
     6121 agcgcctgga attttctggg cagtcggcct gaggtcaagg tggtgcagct cacggtgacg
     6181 accggcaaca gtgcagcgca aagcctgtac cagcagtttg ggtttgttgc gtttggaact
     6241 gagccgatgg cggttgcagt tgccggggct tatgtgtcaa aggtgcacat gtggtgttcg
     6301 gtggaatctt ccagcccgat ccggcatggc ggtgggtgac gcgctttttt tcggataatc
     6361 gaccgtttcg cctggcttct tttctcgaac ttttgatagg acatccccga tgaaagcaat
     6421 ctgcacaaag gcattgcgcc tcaccgtgac tgctgcggca gcgcttacct tggcaagcgg
     6481 gtcggcactg gccgccgaca aggtcaaggt tggcatgctc agcaccctgt ccgggccggg
     6541 tgcggccctt ggcattgaca tccgtgacgg cttcaacctg gcgctcaaac actcgggtgg
     6601 caagtttggt ggtttgccgg tggaagtcat cgtggctgac gatcaggcca gcccggatgc
     6661 ggccaagcaa acggcggacc ggctggtcaa gaaagacaag gtcgatttca tgaccggcgt
     6721 ggtgttttcc aacgtcatgc tggcggttgg caagccgatt tttgactcga agacctttta
     6781 catcagtgcc aacgccggtc cgtcgcagta cgccggtgcc caatgcaacc cgtatttttt
     6841 cagtgcctcg taccaaaacg acaacctgca cgaagcggtt ggcaagacgg tgcaggacaa
     6901 gggcttcaaa aaggtagcgc tgctggcccc caactacccg gcaggcaagg acgcgctgac
     6961 tggcttcaag cgctttttca agggcaccat cgtctctgag gagtacacgc ccctgagcca
     7021 actcgattac ggcaccgagc tgtccaaaat gcgcgcctct ggtgctgatg cggtgtatat
     7081 ctttttgcct ggcggc
//
LOCUS       DAOSLL010000177         7041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9722_length_7041_cov_5.408818, whole genome shotgun sequence.
ACCESSION   DAOSLL010000177 DAOSLL010000000
VERSION     DAOSLL010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9722_length_7041_cov_5.408818"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PLB25_09330"
     CDS             complement(<1..464)
                     /locus_tag="PLB25_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOE41814.1"
                     /translation="MDSKNGVQSLEIGIGILRAISSGHHAMMLKDIAGATGMSASKVH
                     RYLVSLVRCGLVEQDPASSRYDLGPLALSLGLAAVDRLDRVKLGMAAIETLRDEINQT
                     IALAVWSDNGPVIVRSVAPERPITVNVVTGTALPLLSSAGGRVFAAHLPRSET"
     gene            complement(501..1895)
                     /locus_tag="PLB25_09335"
     CDS             complement(501..1895)
                     /locus_tag="PLB25_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HOE41815.1"
                     /translation="MTSSNTSLYAQRRAAVAEKIGPAGIAIIPTSPEQQRNRDSDFQF
                     RHDSYFFYLCGFTEPKAWLLITGDGQSTLFCQPKDREREIWDGLRLGPEAALQTLGVS
                     SAFSVDQLDAKLPGLLDGRDAVWYPFATHKGLETRIDGWLGGLRARVRYGTLAPEQQR
                     DVCGLLDEMRLVKDEHEQSVMRRAAQISAAAHVRAMRHCAGMIRQCQEVREYHLDAEL
                     LHEFRQHGSQYPAYGSIVASGGNACVLHYRADAGLVHSGELVLIDAGCELDGYASDIT
                     RTFPANGIFTGPQRTLYELVLASQQAAIAATKAGARFTDPHDATVKVLAQGMLDVGLL
                     DQNKVGLVEDVIEKRAYFQFYMHRAGHWIGMDVHDCGAYTEPGEVGHFSTRKDALTGE
                     IIKDRPARILRSGMALTIEPGIYVRPAAGVPEQFHNIGIRIEDDAIVTDTGCELITRD
                     VPVSVADIEALMRG"
     gene            2781..3965
                     /locus_tag="PLB25_09340"
     CDS             2781..3965
                     /locus_tag="PLB25_09340"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE41816.1"
                     /translation="MKMKFAAFAFILLLTQRAFSGGPVFIGLDAEFGHKTSSSAQAVQ
                     QGMQIAIDEINQTGGVLDGRKLALVTKDNRSLSAMGVDNLRELAKVPDLVAVFGGKFS
                     PVYIECVPVAQELGMPLLDPWGSADPITEHTHRPSFTFRLSLKDSWAGPAFVRFARQR
                     YKASRLGVLLPNTAWGRSNQLAIERAAASAGSQLVGQRWYNWGDTSLLNQYTELRLAG
                     AQAIILVANEAEGAVLVKEIAALPEIQRLPVISHWGVTGGDFAAMAGDSLRLVDFTVI
                     QTFSFVGLDSPASRRVLLALKTKYQINAADQVKSPVGVAHAYDLTHLLAKAVNRAGST
                     DRAKIRDALEKLGAHDGLVQRYPKPFTTDRHDALSADNVFFARYTADDKLIPISLTPR
                     KH"
     gene            3993..6893
                     /locus_tag="PLB25_09345"
     CDS             3993..6893
                     /locus_tag="PLB25_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE41817.1"
                     /translation="MIRVPWLNENDQSLELRIKRTAFMMTMLVVVVLGTVSMILTLVE
                     IATNQAKAHQSAASVIGEVLSGDINSEMGELRQLSANSLVWTALTDSTGRDAYLKPFL
                     RDRKKKTGGGQIELLDYRGRHVLGDPAISLEPEKLAQAVATVLSEKRSRVEVIVSGNH
                     PGLLTIYPVIFPYTKDAIGALVSVIDIFQPFQKRASALDENTGVALFHKERLIAAYPG
                     KHSSRYSEVTFGLTIDEAIDGGPLSLTVYSVNNPLIGPIFQRLLVSISLAILLGALVW
                     RMSGLMARRMTRRLNLLAAACQNGINGRVNEIPKDQSGDEIGVLARTLRKALLEYEQI
                     NSRLEHLVDEKSLKLIASEDRFRSAIDVIDEAFVIFDPQDRLSYCNEKYRQVYPSIAD
                     VIEPGRTFEEMVRIWKERGQGEPLKGSIDAWVTERVAAHQSGGVLIQQVDNGRWMRSV
                     ERKTTTGHIVGFRVDITDLVGATREAQQANLAKSRFLATMSHEIRTPMNGILGMAQLL
                     LIPSLQEDRRNAYARTILSSGQSLLTLLNDILDLSKIEAGKFQLESTAFAPEAMIHET
                     ANLFAGAAQAKGLQLDSQWHGASDQRYLADSNRLRQMLSNLVGNAIKFTHTGQVRIEA
                     REIDRSENTSVLEFAVNDTGIGIAADKLGLLFKPFSQTDSSTTREFGGTGLGLSIVRH
                     LAHAMGGDAGVSSEPGQGSRFWFRVQVQPITDTPDSRKHDRLALDAKSDNAVTLLHGH
                     VLVAEDHLVNCMVIESLLNSMGLTVSVVHDGQQAVEIIAQVAPDGSSDQPRRVDLILM
                     DLQMPVMDGFVATKRIRQWEADSQNPRLPIIALTADAFEEDRQHCLTVGMDDFLTKPV
                     SLAALKLALARWLPNTPDALALPQPPEPLKPVDFDAFKNLVNQLAPLLEDNKFSAIGL
                     FKSLQHLVKGTNLAAEVDALAPMLQEMHFGQVLTRLRLITHNHDTPASEDST"
BASE COUNT         1522 a         1956 c         2037 g         1526 t
ORIGIN      
        1 gtctcagacc ggggcaggtg tgccgcaaaa acccggccac cggccgaact cagcaggggc
       61 agcgcggtgc cggtgaccac attgacggtg atcggacgct cgggggccac cgaacggaca
      121 atcaccgggc cgttgtcgct ccagaccgcc agcgcgatgg tctggttgat ctcgtcccgc
      181 aaagtttcaa tcgccgccat tcccagcttg acccggtcca gccgatcaac tgccgccagg
      241 cccaaactca aggcaagcgg accaaggtcg tagcgcgagc tcgccgggtc ttgctcgacc
      301 agcccgcagc gcaccaggct gaccaggtag cggtgcacct tggaggccga catgccggtg
      361 gcaccggcaa tgtccttgag catcatggcg tggtggccgc tgctgatggc acgcaagatg
      421 ccgatgccga tttccagcga ctgaacgccg tttttgctgt ccatgttcac gggtggatca
      481 agaacgggtt ggcaaaaagc tcatccgcgc atcaaggcct caatatcggc cacgctgacc
      541 ggcacgtcac gcgtgatcag ttcgcagccg gtgtcggtca cgatggcatc gtcttcgatg
      601 cggatgccga tgttgtgaaa ctgctcgggc actcccgctg ccgggcgcac gtaaatgccg
      661 ggctctatgg tgagcgccat gccggagcgc aaaatgcgcg cgggtcggtc cttgatgatc
      721 tcgccggtaa gcgcgtcctt gcgggtgctg aaatggccaa cttcgccggg ttcggtgtaa
      781 gcaccgcagt cgtgcacatc catgccaatc cagtgaccgg cgcggtgcat gtaaaactgg
      841 aaataggcgc gtttttcgat gacatcttca accagtccaa ccttgttttg gtccaacagg
      901 ccgacatcaa gcatgccttg ggccagcacc ttcaccgtgg catcgtgcgg gtcagtaaag
      961 cgcgcaccgg ccttggtggc tgcaatggct gcctgctgcg aggccagcac caactcgtac
     1021 agcgtgcgct gcggccccgt gaagatgcca ttggccggaa aagtccgggt gatgtcagac
     1081 gcatagccat caagctcaca accggcatca atcaatacca gctcgccgct atggaccagg
     1141 ccggcatcgg cacggtagtg caacacgcaa gcattgccac cggacgcgac gatcgagcca
     1201 taggccggat actgcgagcc atgctggcgg aattcgtgca gcagctcagc atcgaggtgg
     1261 tattcacgca cctcctggca ttgtcggatc atgccagcgc aatgccgcat ggcacgcaca
     1321 tgggccgcgg cgctgatctg cgcggcgcgg cgcatgacgg actgctcatg ctcatctttc
     1381 accaggcgca tttcatcgag caggccgcac acatcgcgct gctgctcagg tgccagcgtg
     1441 ccgtagcgca cccgcgcacg caagccgccg agccagccgt caatgcgggt ttccagcccc
     1501 ttgtgggtgg caaacgggta ccagacggca tctcgaccat ctagcagacc gggcaacttt
     1561 gcatcgagct ggtcgaccga aaaggcgctg ctgacaccca gcgtctgcag cgccgcttca
     1621 ggcccgaggc gcaacccgtc ccagatttca cgctcgcggt ccttgggctg gcaaaacagc
     1681 gtgctttggc catcgccggt gatcagcagc caggctttgg gctcggtaaa gccgcacagg
     1741 taaaagaagt aactgtcatg gcgaaactgg aaatcgctgt cacggttgcg ctgctgttcg
     1801 ggtgacgtgg ggatgatggc gatgccagcg gggccgattt tttcggccac tgcggcgcgg
     1861 cgctgggcgt ataggctggt gttgctggaa gtcatgtatg agtatgggtc ggttatcaag
     1921 gcgtcaaatc aatcagtatc tggcagtatt gtgatggctg ggtgcatgtt gccaccaatc
     1981 gcgtgaactt cagccgtgca agcgcacttg cgatgctgcg tggggcaatg cattggccgc
     2041 tgcggctttc ggcctggcag gtgatcgact gaaaagaccc gggcctaagc cggccgagcc
     2101 ggaatcgatg agccttcgtc gcgagggcgc gcctcctaca caaacagtcg gcgccaattt
     2161 gaatttgtag gagtcgcgcc ctcgcgacga atgactttga atgtttgaat tcttcgcatg
     2221 atgtgtaaag acttgacttg ttagagatta aaagccttac ccggcagcac taccacgctg
     2281 tccgaggcaa gtggtacggc tcgctctgaa gttgccactt gtcgtgggca acttgatcac
     2341 cggaatttga tattccaatt gagcggctgg gagcctgccg gacttaggca accttggcgg
     2401 cgaaccgacc gtagcggccc attttgcacc ggtcttttgt cccacaactg ggctatgtgt
     2461 ctgccaattc agcacaaact ggccgcgccg ctggggttga ttttcgctgc gcgatccatg
     2521 tgtccgacag acttatggcc cgacccttca agcaacaggt gtcgtctgtc gtcgcagcgt
     2581 gcaatcgatt tgtctattga gttagatcaa agatagccca ccggactctg gtaaaacgtc
     2641 taccattgac atcagggact ttcccattgg tcaagtcagc agcgttgttg gttcgttgcc
     2701 gagagccccc aaaccgatca aaggtcgaga agcttcgcaa ggttgtgcca gtgattgaga
     2761 attcgcattt gaggctcgtc atgaagatga aatttgctgc ttttgccttc atccttctgt
     2821 tgactcagcg agctttttca ggcgggcctg tttttattgg ccttgatgca gagttcggtc
     2881 acaaaaccag ctcatcggct caggcagttc agcaaggaat gcaaattgcc atcgatgaga
     2941 tcaatcaaac tggtggcgtt ttggatggac gcaagctcgc attggtcacc aaggacaatc
     3001 ggtccttgtc ggcaatgggc gtcgacaact tgcgtgaact ggccaaggtc ccggatttgg
     3061 tcgccgtctt tggtggcaag ttcagtccgg tttatatcga atgcgtacct gttgctcagg
     3121 aacttggtat gccactgcta gacccatggg gttcggctga cccgatcacc gagcataccc
     3181 atcgaccaag ctttactttt cgcctgtctt tgaaggacag ttgggctggt ccggcttttg
     3241 tccgtttcgc cagacaacga tacaaggcaa gcaggcttgg ggtgctactg cccaacaccg
     3301 cctggggacg aagcaatcag cttgcgatag aaagggccgc agcatcggca ggcagtcaac
     3361 tggttgggca acgttggtac aactggggcg acacctcact gctgaaccaa tacactgaac
     3421 tgcgtttggc aggtgcccaa gccattattc ttgtcgcgaa cgaagccgaa ggcgccgttt
     3481 tggtcaagga gatcgccgct ctgccggaaa tccagcgctt gccggttatc tcgcattggg
     3541 gcgtgacagg tggcgatttc gccgccatgg ccggagactc actgagattg gtggatttta
     3601 cggtgattca aaccttcagc ttcgttgggc tcgattcgcc tgcatcccgg cgcgtcctgc
     3661 tggcgctgaa aacgaagtac cagatcaacg ctgcagatca ggtgaaaagt ccagtcggcg
     3721 ttgctcatgc ttatgatctc acccatcttt tggcgaaagc agttaaccgg gctggaagca
     3781 cggaccgggc caagattcgc gacgcgttgg aaaaactcgg tgcccatgat ggcttggtac
     3841 agcgataccc caaacctttt acaacggacc ggcatgatgc cctttctgcg gacaacgtgt
     3901 tttttgcccg atatacggca gatgacaagt tgattccgat ctcactcaca ccccgaaagc
     3961 actgatatgt tcttgaatga gcggagttcg gtgtgatccg ggttccctgg ctaaacgaga
     4021 atgaccagtc gctagagctg cgcatcaagc gaaccgcgtt catgatgacc atgctggttg
     4081 tagtggtcct gggtaccgtc tcaatgatcc tgactttggt ggagattgcg acgaatcagg
     4141 caaaagcaca ccaaagcgct gcgagcgtga ttggtgaggt tctctcagga gacatcaaca
     4201 gcgaaatggg tgaactcaga caactgagtg caaactcttt ggtctggacc gccttgactg
     4261 attcaaccgg tcgcgatgcc tatctcaagc cattcttgcg cgacagaaag aaaaagaccg
     4321 gcgggggaca gattgaactg ctggattacc gtggacgcca cgttttgggc gatcctgcaa
     4381 tcagccttga accggaaaag cttgctcagg cagtggccac cgtgttgtcg gaaaaacgct
     4441 cccgagttga agttatcgtg agtggcaacc atccaggctt gctgaccatt tatccggtta
     4501 tcttccctta cacgaaggat gccatcggtg cgctggtgag tgtgattgac atcttccagc
     4561 cgtttcaaaa gcgtgcatcg gcgcttgatg aaaacaccgg tgttgcccta tttcacaagg
     4621 aaaggctcat tgcagcctat ccaggcaagc attcaagtcg gtattcagag gtaactttcg
     4681 ggttaaccat tgacgaagca attgacggcg ggccgctctc tttgacggtc tattccgtca
     4741 acaacccgct gatcggaccg atcttccagc gtttgcttgt gtcaatttcg ctggccattc
     4801 ttcttggtgc gctggtatgg cgcatgtccg gtttgatggc tcgacggatg acgcgacgcc
     4861 ttaacctatt ggctgcagct tgtcagaatg ggatcaatgg gcgggtcaat gaaattccga
     4921 aggatcaatc tggtgacgaa attggcgttt tggcacgcac tttgcgcaag gcactcctgg
     4981 agtacgagca gatcaacagt cgcctggagc atctggttga cgagaagtca ctgaaactga
     5041 tagcaagcga agacaggttt cgcagcgcca ttgatgtgat cgacgaggcc tttgttattt
     5101 ttgaccccca ggaccgactg tcatattgca atgaaaagta ccggcaggtg tatccatcga
     5161 tcgccgatgt gattgagccg ggacgaacat ttgaagaaat ggtacgtatc tggaaagaac
     5221 gcggtcaagg cgagcccttg aaggggagca ttgatgcatg ggtgacagag cgggttgctg
     5281 cccatcaaag tggcggtgta ttgattcagc aggttgacaa tggtcgttgg atgcgaagcg
     5341 tcgaacgcaa gacaacgact ggtcacatcg tcggctttcg ggttgacatc actgacttgg
     5401 ttggcgcgac ccgcgaggcg cagcaggcaa atttggcaaa aagccgattt ctcgcaacca
     5461 tgagccatga aattcgcacc ccgatgaacg ggattctggg aatggcgcaa ctgctgctga
     5521 tacccagtct gcaagaagac cgccgcaatg cctacgcccg caccattctg tcgagcggcc
     5581 aatccttgct gacgctgctc aacgacatcc tggatttgtc aaaaatagaa gctgggaagt
     5641 tccagttgga gagcaccgcg tttgcccctg aggcaatgat ccatgaaacc gccaacctgt
     5701 tcgccggagc ggcgcaggcc aagggtttgc aacttgacag ccagtggcat ggtgcgtctg
     5761 atcagcgcta tttggctgat tcaaatcgcc tgcgccagat gctgtccaac ctggtcggca
     5821 atgccatcaa gttcacgcac accgggcaag tgcgcattga agccagggag attgatcgca
     5881 gcgagaacac cagcgtgctg gagttcgccg tcaacgacac cggcattggc atcgcagccg
     5941 acaagctggg tttgctgttc aagccgtttt cgcaaaccga cagttcgacc acccgtgagt
     6001 ttggcggcac ggggctgggt ttgtcgattg ttcgccatct cgcccatgcc atggggggcg
     6061 atgcgggtgt cagcagcgag ccaggccagg gctcccggtt ctggtttcgg gtgcaggttc
     6121 aaccgatcac cgacacgcca gacagtcgca aacatgaccg actggcgctg gacgccaaat
     6181 ctgacaacgc cgtcactctg ctgcacggtc atgtgctggt ggcagaagac cacctggtga
     6241 attgcatggt gatcgaatcc ttgttgaaca gcatgggctt aaccgtgtct gtggtccatg
     6301 atggccaaca ggccgttgag atcattgcgc aagttgcacc cgatggcagc agcgatcaac
     6361 ccaggcgggt tgatctgatt ttgatggact tgcagatgcc agtgatggac ggctttgttg
     6421 ccaccaagag gatcaggcaa tgggaggcgg atagccagaa cccacgcctg ccgatcattg
     6481 ccctgaccgc tgatgctttt gaagaagacc gtcaacattg cctgacagtt ggcatggacg
     6541 atttcctgac caagccggtc tcacttgcgg cactgaaatt ggcacttgcc aggtggctgc
     6601 ccaacacacc tgatgcgctt gccctgccgc agccacccga gcccctcaag ccagtggact
     6661 ttgatgcgtt caagaatctt gtcaaccagc ttgcgccctt gctggaagat aacaaattct
     6721 ctgccattgg actcttcaag tcgctccagc atcttgtcaa gggcacaaac ttggcagcag
     6781 aagtggatgc tctggcaccc atgttgcaag aaatgcactt cggccaagtg ctgacgcgcc
     6841 tgcgcctcat cacccacaat cacgacacac ctgccagcga agactcgaca tgacggccca
     6901 acacgcacgc attctcgtca ttgatgacac accagccaac ctgctgacac tgtgttcggt
     6961 gctggagggc gagtttgagc tgcaatttgc cacctccggc ccgcaaggga ttgcgttggc
     7021 cctcaagaat ccgcctgacc t
//
LOCUS       DAOSLL010000178         7032 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9739_length_7032_cov_4.876165, whole genome shotgun sequence.
ACCESSION   DAOSLL010000178 DAOSLL010000000
VERSION     DAOSLL010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7032
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9739_length_7032_cov_4.876165"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /gene="tsaE"
                     /locus_tag="PLB25_09350"
     CDS             complement(<1..250)
                     /gene="tsaE"
                     /locus_tag="PLB25_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HOE41818.1"
                     /translation="MCSEAVHLPIVKTALWHNEDATRQFAQTLAAQAALRHAFIELRG
                     ELGAGKTTLVRHLLRALGVQGRIKSPTYAVVEPYELPGL"
     gene            278..1336
                     /gene="queG"
                     /locus_tag="PLB25_09355"
     CDS             278..1336
                     /gene="queG"
                     /locus_tag="PLB25_09355"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HOE41819.1"
                     /translation="MQHWGLQLGFSQIGIAGIDLASAEPGLSAWLAQGFQGDMAYMAR
                     HGLKRARPAELVPGTLSVITARMDYLPVTTPAQWQVAELDRLGAPAQAIVSVYARGRD
                     YHKVLRSRLQSLAEHIATGLSPLACRVFTDSAPVLEAELAARSGQGWRGKHTLLLNRS
                     GGSMFFLGEIYVDVALPPTAPVASHCGSCQACLEVCPTKAIVAPYQLDARRCISYLTI
                     EHPGPIAPELRALMGNRIYGCDDCQLICPWNKFARRSELADFDARAALVNRPLAALFA
                     WSEADFLHFTQGSPIRRIGHLRWLRNIAVALGNALRVASALPETRRELAAVLQTRLEH
                     PSPLVREHVVWALEAVRP"
     gene            complement(1831..1906)
                     /locus_tag="PLB25_09360"
     tRNA            complement(1831..1906)
                     /locus_tag="PLB25_09360"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1871..1873),aa:Ala,seq:cgc)
     gene            complement(2481..2840)
                     /locus_tag="PLB25_09365"
     CDS             complement(2481..2840)
                     /locus_tag="PLB25_09365"
                     /inference="COORDINATES: protein motif:HMM:NF039512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5710 domain-containing protein"
                     /protein_id="HOE41820.1"
                     /translation="MIGLGNGWSLHLATPFAEKDAVKALGARWDASKKCWYIVDMADL
                     TPFARWIPNLAAALAVSGTTTQATKQKLNLPAPGGVSAPVNTQALIRAPANTNAGVAD
                     CGCHALPREDCVQSAKS"
     gene            2941..3783
                     /locus_tag="PLB25_09370"
     CDS             2941..3783
                     /locus_tag="PLB25_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008919087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOE41821.1"
                     /translation="MLSPACQSYAGPLHNDDHTDMAKFEDYCNSYKHLHMQRNDGILE
                     VTLHSNGGELVWGAGPHRECGAAFGDIAADPGNRVVILTGSGSEFIAQESISHPGITA
                     PVWDRVLVHAQRLIYNHLEIRVPMIAAINGPATIHAELGLLCDIVLASDDAMFQDAPH
                     YGEGLVPGDSVQVLWPLLLGMNRGRYFLLTGQQLDAAQALALGVVSEVMPRVALMERA
                     RWHARKLLKQPPMTVTYARLAITHELKRLMRDHLDYGLLLEGLAATEYFPGRLAHHSG
                     EAQE"
     gene            3827..4003
                     /locus_tag="PLB25_09375"
     CDS             3827..4003
                     /locus_tag="PLB25_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41822.1"
                     /translation="MSTACDRIKRGLQQAIEHTEGCAPQARIRCPGPVTTACPLEEFT
                     QRQSQIAQSHPAVH"
     gene            4223..4534
                     /locus_tag="PLB25_09380"
     CDS             4223..4534
                     /locus_tag="PLB25_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41823.1"
                     /translation="MRNILLGMPPLPEQTQIAAFLDRETAKIDALVAEQRRLMALLKE
                     KRQAVISHAVTRGLNPNAPLKPSGIDWLGNVPEHWEMKPLRMIANVVRGASPRPAEFE
                     Y"
     gene            4539..4733
                     /locus_tag="PLB25_09385"
     CDS             4539..4733
                     /locus_tag="PLB25_09385"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41824.1"
                     /translation="MKDLRFGFPPLQEQELIVCRITELVNEFDALTAEAQRAIDLLQE
                     RRTALISAAVTGQIDVRGAA"
     gene            4913..6766
                     /locus_tag="PLB25_09390"
     CDS             4913..6766
                     /locus_tag="PLB25_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004554541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HOE41825.1"
                     /translation="MARYATNRLRVVRQVRYSLHNENSIDVVLFLNGLPVATVELKTD
                     FTQSIGDAIDQYRFDRQPKPKGQGAEPLLTFPSGALIHFAVSNQEVAMVTQLAGPATV
                     FLPFNLGDDGAAGNPVNAAGGHRTAYLWEQVWARESWLEILGRYLIARRNKKKQIEAI
                     IFPRYHQLDVTRKLQAAILVEGPGGKYLIQHSAGSGKTNSIAWTAHFLAELHDAKNQK
                     VFDTVLVVSDRNVIDVQLQEALFDFQRTTGVVATITNQDGSKSGRLAEALSGDKKIVV
                     CTIQTFPFALEAVRRLAATQGKRFAVIADEAHSSQTGEAASKLKAVLSPEELQELNDG
                     GEISTEDILAAQMASRAEDGGITFVAFTATPKNKTLELFGTRPDPARKPAADNLPKPF
                     HVYSMRQAIEEGFILDVLQNYLRLYTFLSQIFDYGNTGIEKRAIFYKRLLPLLEFGRE
                     REGIDLSKVVLTHHHLKDQGARTLPLSNGETPKLAPITEAGSGSVQEQQKVYMAELIS
                     KLNDLFGNDTSEQDQLRYVNGTILGKVSESRILQQQAANNTREQFANSPDLHTELQNA
                     IIESYDAHTVMSTQALNSPIVLRGILDVLLNHAGLYETLRARETGRDRQYG"
BASE COUNT         1512 a         2080 c         2066 g         1374 t
ORIGIN      
        1 tcaggccggg cagttcatag ggttcaacca ccgcgtaggt cgggcttttg atgcgtccct
       61 gaacccccag cgcgcgcaac agatgccgca ccagcgtggt cttgcccgcg ccaagctcac
      121 cacgcaattc gatgaacgca tggcgcaggg cggcttgtgc cgccagggtc tgggcaaatt
      181 gccgggtggc atcctcgttg tgccagagcg cagtttttac aatggggagg tgaacagctt
      241 cagaacacat atcgatggtg gcttgtgggt ctccagaatt caacactggg ggcttcagtt
      301 gggattctcc caaattggca ttgccgggat cgatttggcc agtgcagagc ccggattatc
      361 tgcgtggctg gcgcaaggct ttcagggcga catggcttac atggcacggc acggcctgaa
      421 acgcgcccgg ccggccgaac tggtgcccgg caccttgagc gtgatcaccg cgcgcatgga
      481 ctatctgcca gtcactacgc cagcacaatg gcaggtggcg gaattggacc ggctcggggc
      541 gccggcacag gccatcgtgt cagtttatgc ccgtggccgt gattaccaca aggtgttgcg
      601 cagccgcctg caaagtctgg ctgaacacat cgccaccggg ttgtcgccgc tggcttgccg
      661 ggttttcacc gactcggcgc cggttctgga ggccgagctg gcagcccgca gtggccaggg
      721 ctggcgcggc aaacacacgc tgctgctcaa tcgcagcggc gggtcaatgt tttttctggg
      781 tgaaatttat gtcgatgtgg cactgccgcc cactgcgccg gttgcaagcc attgcggcag
      841 ttgccaggcc tgccttgagg tttgcccaac caaagcgatt gttgcgccgt accagttgga
      901 tgcacggcgc tgcatttcct acctgacgat cgagcaccct ggaccgattg cgccggagct
      961 gcgcgcgctg atgggcaaca gaatttatgg ctgcgacgac tgccagctca tttgcccttg
     1021 gaacaaattt gccaggcgta gcgaattggc tgactttgat gcccgcgccg cgttggtaaa
     1081 tcgcccgctg gcggccttgt ttgcctggag cgaagcagat tttttgcatt tcacgcaggg
     1141 cagcccgatt cgccgcattg gccacctgcg ctggttgcgc aacattgccg tggcgctggg
     1201 caacgcgttg cgggtggcaa gtgccctccc cgagacacgc cgtgaactcg cagccgtgct
     1261 gcaaacgcgc cttgagcacc ccagcccgct agtgcgcgag catgtggtct gggcgctcga
     1321 agccgtccgg ccgtgatcgt tgtccttggc cgatggcctg aatcggacgg agatcgcccg
     1381 gccaagcacc agccgatggg gattgtgggt acgttgtgga cacctcgcgc agtgcagcgc
     1441 caaaacattg gtctgcatgt tgtcccttat gctccaaatc accaccagcc gaaaaccttg
     1501 agtctgatct ggcgaaagag caacttgccg ccgtcggact gtgatgcgca gcaaaggttg
     1561 attgacgaat tcgatgcgta gaacaggttt gcaatgtttc tggtcaacgc agttgccgcc
     1621 caaccatcgg cctgaaaccc gcatgagatc gaagggtcga acatcgggca gccacaggtc
     1681 aaatggaaaa aagaggggat tctgggtgtt ggcggacagg aaaacggggg catttccgac
     1741 tggccgacaa cgcaccgccg cccgggaccg cgccagtact ggcgcgacag gcagaaaaaa
     1801 gcccaaccga taagggttag gctttttaat tggtggagag gatggggatc gaacccacga
     1861 cctctgcatt gcgaacgcag cgctctccca actgagctac ccccccaatg ccaagagtct
     1921 atcaatatta acgaaagcag acaactctgg agttcagatg gtcacgggta aaggtcgctc
     1981 agagagatcg ttcgaacaaa ccagacctga tgctgggtca acgtcgagac cagcatccaa
     2041 gcgcaccaaa ctgaacaaaa accattgtga caagtgccca ataaagttgt tgttgactat
     2101 gcaaaacaac tggcgcagaa ttcactcgtg accgagtttt caccctcggt gaaggagccc
     2161 cgtagatgat caaggacaac tcttacgctc acacctgatt gacggtttgc cgtcatgacg
     2221 tttcccatac ctgaggaagc acacaaaaag ggtaggattc ttttggattc tgcccttttt
     2281 tgtgaccgga gatttcctgc gggcctgcaa ccagtgcggg tttttggcag acagccgagc
     2341 atgccccacc gacccaagca ccgaacccac aaccgaagca tctgcccaac ccatgccgca
     2401 tcaccgggca acacgctcgg gcaccaaaca ggcgcgtccc agaaagacaa atcccccaag
     2461 cgacgacgca actcgcagat tcatgacttt gcggattgca cgcagtcttc ccgaggcagt
     2521 gcatggcaac cacagtccgc cacgcctgca tttgtatttg ccggagctct gataagtgct
     2581 tgagtgttga ccggtgctga aacgccgccc ggtgcaggca ggttgagctt ttgcttcgtt
     2641 gcttgcgttg tcgttcccga aacagccagc gcagcagcca agtttggaat ccatcgtgca
     2701 aacggcgtga gatctgccat atcaacgata taccaacact ttttcgaggc atcccagcgg
     2761 gcaccaagtg ccttgaccgc gtctttctca gcaaagggag tggccagatg caaactccac
     2821 ccattgccaa gcccgatcaa tcgtcttcat catctgcatc cggcatcacc gcatcggcaa
     2881 cgacacccac ctccttttca gtgactttcg gctggttacc ccgaggatcg gcaacaattg
     2941 gtgctttctc cggcctgcca gtcgtatgcc ggaccacttc acaacgacga tcacaccgac
     3001 atggctaagt ttgaagacta ttgcaactcc tacaaacacc tgcacatgca gcgcaacgat
     3061 ggcattctgg aggtcacgct gcacagcaac ggaggcgaac tggtctgggg cgcaggccca
     3121 cacagggaat gtggtgccgc gtttggggac attgccgccg accccggtaa ccgggtggtt
     3181 attctgacag gcagtggcag cgagttcatt gctcaggagt ccatctcgca ccccggcatc
     3241 accgcgcccg tgtgggatcg cgtgctggtt catgcgcagc ggctgattta caaccacctt
     3301 gaaattcggg tgccgatgat tgccgcaatc aacggcccag ccaccatcca tgccgaactt
     3361 ggcctgttat gcgacatcgt gctggcatca gatgatgcga tgtttcagga tgcaccgcat
     3421 tacggcgagg gcctcgtgcc cggcgacagt gtgcaggtgc tgtggccctt gttgttgggg
     3481 atgaaccgtg ggcgctattt cttgttgacc ggtcagcagc ttgatgccgc gcaggcgctg
     3541 gcactgggcg tggtgagtga agtcatgccg cgtgtcgcat tgatggagcg cgcccgctgg
     3601 cacgcccgca aattgctgaa acagccgccg atgaccgtca cctacgcccg gttggccatc
     3661 acccacgaac tcaagcgcct gatgcgtgac cacctcgact atggcctgct gctcgaaggc
     3721 ttggccgcga ctgagtattt tccgggcagg ctggcgcacc attcgggtga agcgcaagaa
     3781 taggccggac tgcaattggg cctgtcacgc tgtgccaacg cttgacgtga gcacagcctg
     3841 cgaccgcatc aaacgcggct tgcagcaggc cattgagcac accgagggtt gtgcgccgca
     3901 ggcgcgcata cgctgtcccg gtccggtgac caccgcctgc ccgctagagg aattcaccca
     3961 acgccaaagc caaatcgcgc aaagtcaccc tgctgttcat tgaagacgat gcggcgctga
     4021 ccaagccggg cgtggtgcgc agcctgtaag accccaccgc aggcaccggc ggcatgctca
     4081 gcgtggctgg tgaacatctg accagcctga accccgatgc ccggttggtg atgtacggcc
     4141 aggaactcag ccatttcaag aagagtttta tcgctatggc aaaggaatag ttgccgacct
     4201 ttggagtacg aactattccg agatgcgaaa cattctgctc gggatgccac ctctccccga
     4261 acaaacccaa atcgccgcct tcctcgaccg cgaaaccgcg aagattgatg cgctggtggc
     4321 agagcagcgg cggctgatgg cgttgctgaa agaaaagcgc caggccgtca tctcccatgc
     4381 cgtcactcgg ggcctgaacc ccaacgcccc cctgaagccg tccggcatcg attggctggg
     4441 gaatgtgccg gagcattggg agatgaagcc cttgcgcatg attgccaacg tcgttcgtgg
     4501 cgcatccccg cgtccagccg aatttgaata ctgacatcgt gaaggacctg agatttggat
     4561 tcccaccgct gcaagaacaa gaattgatcg tttgccggat cacagaactg gtgaacgagt
     4621 tcgatgctct caccgccgaa gcccaacgcg cgatcgatct gctgcaagag cgccgcaccg
     4681 cgctgatctc cgctgctgtc accggccaga ttgatgtgcg gggtgcggcg tagtaactat
     4741 ggtttcaata gctactagcg catattccac aagggctaga ggccgatatg gcacttaatt
     4801 agtccctggc aagtcaagtc tttgcatgtc atgcgaagaa ttcaaacatg caaagtcatt
     4861 cgtcgcgcgg gcgcgactcc tagtgcgccc gtgaaggcgc tacgttagca cggtggcccg
     4921 ctacgccacc aaccgcctgc gtgtggtgcg ccaggtgcgc tactcattgc ataacgaaaa
     4981 cagcattgat gtggtgctgt tcctcaacgg cctgccggtg gccacggtcg aactcaagac
     5041 cgacttcacc caaagcattg gcgatgctat tgaccaatac cgctttgacc gccagcccaa
     5101 gcccaaaggg cagggggctg aacccttgct gacgttcccg tccggtgcct tgatccactt
     5161 tgccgtcagc aaccaagagg tcgccatggt cacccaactg gctggcccgg ccaccgtgtt
     5221 tctgcccttc aacctgggtg acgatggcgc agcaggcaac ccggtcaacg ccgccggtgg
     5281 tcaccgcacc gcttatttgt gggaacaggt ctgggcgcgt gaaagctggc tggagatact
     5341 gggccgctac ctgatcgcaa ggcgcaataa gaaaaagcag attgaggcca tcatcttccc
     5401 gcgctaccac cagttggacg tgacgcgcaa actgcaagcg gccattctgg tcgaaggccc
     5461 cggcggcaaa tacctgatcc agcacagcgc aggttcgggc aaaaccaact ccatcgcctg
     5521 gactgcgcac tttctggccg agctgcatga cgcgaaaaac cagaaggtgt ttgacaccgt
     5581 gctggtggtg tctgaccgca acgtgatcga cgtgcagttg caagaggcgc tgtttgactt
     5641 tcagcgcacc accggcgtgg tggccaccat caccaaccag gacggcagca agagcggcag
     5701 attagccgag gcgctgtcgg gcgacaagaa gatcgtggtt tgcaccatcc agacctttcc
     5761 gtttgcgctg gaggcggtgc gcagactggc cgccacacag ggcaagcgct ttgccgtgat
     5821 tgccgacgag gcgcacagct cgcagaccgg tgaagccgcc agcaaactca aggccgtgct
     5881 gagccccgaa gaactgcaag agctgaacga cggcggcgag atcagcaccg aggatatttt
     5941 ggccgcgcag atggccagcc gcgccgaaga cggtggcatc acctttgtgg cctttaccgc
     6001 cacgcccaag aacaagacgc tggagttgtt tggcacccgg cccgaccccg cacgcaaacc
     6061 ggcggctgac aacctgccca agccgtttca cgtctattcg atgcggcagg cgattgaaga
     6121 aggcttcatc ctggacgtgc tgcaaaacta tctgcggctg tacacctttt tgtcgcaaat
     6181 cttcgactac ggcaacaccg gcatcgaaaa gcgcgccatc ttttataagc ggctgctgcc
     6241 gctgctggag tttggccgcg agcgtgaagg cattgacttg tccaaggtgg tgctgacgca
     6301 ccatcacctg aaagaccaag gtgcgcgcac tctgcctttg tccaacggtg aaacacccaa
     6361 attggcccca attaccgagg ctggcagcgg cagcgtgcag gaacagcaga aggtgtacat
     6421 ggccgagctg atcagcaagc tcaatgacct gtttggcaat gacaccagcg agcaggacca
     6481 gttgcgctat gtgaacggca caattttggg caaggtcagc gaatccagga tcttgcagca
     6541 gcaggccgcc aacaacacac gagagcagtt tgccaattcg cccgacctgc acaccgagct
     6601 gcaaaacgcc atcatcgagt cgtatgacgc gcacacggtc atgagcacac aggcgctgaa
     6661 ttcaccgatt gtgttgcgag gcattctgga tgtgctgctg aatcatgccg ggctgtatga
     6721 gacgctgcga gcgcgggaaa cagggcgtga ccgccaatat ggctgaacgg cgacaagtag
     6781 tcgccggaat tgcaggatgc tgcgctggag ctggtgctgc agcaggggtg ggtgatgggg
     6841 gagagttagt ctgcgaatga ggtggcgctg gctgatgcgt cgcgccttta gctcgaacga
     6901 taggggaaac ctatgaatga cgactgggat ggtgcggact gggccggttg ggattgcggc
     6961 tctatgacgc tacttttcaa ctggacagtg cgtagttccc cccttcgtgt tcaggccgcc
     7021 attggctgcg ca
//
LOCUS       DAOSLL010000179         7028 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9748_length_7028_cov_8.879535, whole genome shotgun sequence.
ACCESSION   DAOSLL010000179 DAOSLL010000000
VERSION     DAOSLL010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7028
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9748_length_7028_cov_8.879535"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..531)
                     /gene="pcaC"
                     /locus_tag="PLB25_09395"
     CDS             complement(154..531)
                     /gene="pcaC"
                     /locus_tag="PLB25_09395"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HOE41826.1"
                     /translation="MDANQFEAGLAMRKQVMGEDFVARAFAGVTDFTAPLQQHITRNA
                     WGDVWQRPGLDLKTRSLVTVAMLTALGKQHELKGHVRGALNNGASVQEIQEVLLHATV
                     YCGIPAAVEAFRSAAEVVAAVDA"
     gene            881..3511
                     /gene="mutS"
                     /locus_tag="PLB25_09400"
     CDS             881..3511
                     /gene="mutS"
                     /locus_tag="PLB25_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HOE41827.1"
                     /translation="MSEESSEGAGKHTPMMQQYLGLKAAYPDTLLFYRMGDFYELFYA
                     DAEKAARLLNITLTQRGQSAGQPVVMAGVPFHAVDTYLARLIKLGESVAICEQVGDVA
                     SAKGPVERKVVRVVTPGTLTDLELLSDKSEAILLSVQQGTRNQCGLAWLNVTQGVVHL
                     AECLADDLAAWVLRIGAGELIFSAGATPAFETRLRAIQASPALSVSVRPDWQFDAGLG
                     QRTLLAHLQAASLAAWQAQDLAHAHAAAAALLSYAEHTQGRSLTHIQSVRVQRADDLI
                     DLPQTTRRNLELTQTLRGEDAPTLFSLLDTCMTGMGSRLFKSWLLSPSRDRTEATARH
                     RAIEALRSGAAMSAGVGPWARLREQIKGSTDVERITARIALRQVRPRELVGLHATLQK
                     TELLAPVLYGLDAYLTQISEQLQSPAGCAELLRQAIAAEPAALVRDGGVIASGYCAEL
                     DELRAIQTNCDGFLLDLEGRERLRTGIANLRVQYNKVHGFFIEVTQGQIDKVPTDYRR
                     RQTLKNAERFITPELKAFEDKALSAQERALAREKWLYDQVLDQLQAHVPALARLARAL
                     AALDVLCALAERSLTLDWNAPQFVKEPCIDIEAGRHPVVQARLAELSNGNFIANDTRM
                     GPRQRMQIITGPNMGGKSTYMRQIAVIVLLASMGSYVPAKSCRLGPLDAIHTRIGAAD
                     DLANAQSTFMLEMLEAAQILNAATPNSLVLMDEIGRGTSTFDGLALASAIATQLHDKT
                     QAYTLFATHYFELTEFPATHHAAINVHVSAAESGRDIVFLHEIQSGPASKSYGIAVAR
                     LAGMPAAVLKHARHTLEALELQANQHQAQVDLFAVPQATEVVAVSAVETWARALNPDD
                     LSPREALEALYQLKKLSAAS"
     gene            4002..4265
                     /locus_tag="PLB25_09405"
     CDS             4002..4265
                     /locus_tag="PLB25_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41828.1"
                     /translation="MKTSIIKWMAVAAVMVSGQAFAGADDDAWIAKCVKDNAKEGAKA
                     DVVKKYCTCMNSKMDDNETKSITQWEKTHPDEMKACEKVAGWK"
     gene            complement(4762..6105)
                     /locus_tag="PLB25_09410"
     CDS             complement(4762..6105)
                     /locus_tag="PLB25_09410"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOE41829.1"
                     /translation="MKTFLQHLLPRLFGAAPAQSATEAAGALANPPDPQAGTVPEVQI
                     TGPRRPLIDSDGAIAGFEFHINDHILDRLGHRADSLGRGAHVAALLTSARLLAASGRA
                     GLARVPVDWLAHAVTVEVSAGVWVGIEPATEQHHENLLVPAARELVQQLRTAGAKVGW
                     DPACAWDLTPDFALLTAGSEPMAKLLQRQQSWPAELQNLPIVVTEVADVEDLEMALCH
                     GVHFACGVLAPAGITSAARGLLPLPPEVHRVGHLLHQLDDGSDTALIVSGIKSDVGLS
                     YDLLKRINSASFAQIKADASIDQAVLLLGRNALYRWLSMLLMRFAGRRKVSSALQEVT
                     LWRARLLERLAQESHEPAPDQLFTLGLASMLSLILKISPQDVVSTLNLPSAAQQALLD
                     QSGPWFVYLQTVWQVESHQLDEANELVARLGGVAQVTALADEAWAWAWAAEHENR"
     gene            complement(6321..6728)
                     /locus_tag="PLB25_09415"
     CDS             complement(6321..6728)
                     /locus_tag="PLB25_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41830.1"
                     /translation="MSDTIWVRRKSLAQTDDAGDDYDHSLFCKHSQALDKLSDALAIR
                     KLSDFFDTTDLEFNLSEDDLPDSWIAENEKWFQASQALPALRTIIESLKAGPAKGIKE
                     QIRPELLEELADCEAKLAAAESAGDLFHFCIVM"
     gene            complement(6843..>7028)
                     /locus_tag="PLB25_09420"
     CDS             complement(6843..>7028)
                     /locus_tag="PLB25_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41831.1"
                     /translation="NSKAVVAIELRLERAAVNLLTRTFEAEQPASDESIGTFVVAMEQ
                     ALGKIYVQFLADIAQIR"
BASE COUNT         1493 a         2118 c         2082 g         1335 t
ORIGIN      
        1 gctgtgcgcc caaatccgcc ggtgcaaata ggggaagttt tttctgacca catccttagc
       61 aaccacaaat gtttgaacgg gcaaggaccg gcgctgcggc aaagccaatg acctggccag
      121 atccaaacca gcgcgtacca tgagccaaag caatcaggca tcgaccgcag cgacaacttc
      181 ggcggcactg cgaaaggctt caaccgccgc cggtatgccg caataaaccg tcgcgtgcag
      241 cagcacctcc tggatttcct gcacgcttgc accgttgttc aatgcgcccc tgacatggcc
      301 cttgagttcg tgctgtttgc ccaacgcagt cagcatggcc acggtaacca ggctgcgggt
      361 tttcagatcg agacctgggc gttgccacac gtcgccccag gcgttgcggg tgatgtgctg
      421 ctgcagcggg gcggtgaaat cggtgacacc ggcaaatgca cgcgccacaa aatcttcgcc
      481 catgacctgc ttgcgcatgg ccaggccagc ttcaaattga ttggcatcca tcagatcgac
      541 tccaaaagcg tcaaagcata ctggttttca aatggagacc ggcctgccga ggggcaggca
      601 gtcggcccgc aatgcccgag gcagccgatg gcagcgagca aatcccgatc ggcaggcacc
      661 gatgcaagga ggcgagggcc gatgaatatg tggcatgctt ggagaatgtc cagaatgtcc
      721 agaatgtcca gaatgtccag aatgtccacc cgcctggtaa ctgcctggca agtttggctg
      781 gcgcaaaatc cgtgctgctg gctgtgcgga caggcctggc gacaccctgt caattccgcc
      841 caggtaacgg cgcacattgg ttgccctata aagaaaaagc atgtctgaag aatcttctga
      901 aggagccggt aaacacaccc cgatgatgca gcagtacctc gggctcaagg cggcgtatcc
      961 tgacaccctg ttgttctacc gcatggggga cttttacgag ctgttttatg ccgatgccga
     1021 aaaggccgcg cgcctgctca acatcaccct gacgcagcgc ggccaaagcg ccgggcaacc
     1081 ggtggtgatg gccggggtgc cgtttcatgc ggtggacacc tatctggcgc gactcatcaa
     1141 gctgggcgag tcggtggcga tttgtgaaca ggtgggcgac gtggccagcg ccaaaggccc
     1201 ggtcgagcgc aaggtggtgc gggtcgtcac gccgggcacg ctgacagatc tggaactgct
     1261 gagcgacaaa tccgaggcca ttttgctcag cgtgcaacag ggcacgcgca accaatgcgg
     1321 cctggcctgg ctcaacgtga cgcaaggcgt ggtgcatctg gccgaatgtt tggcagacga
     1381 cctggccgcc tgggtgctgc gcattggcgc tggcgaactg attttcagcg ctggtgccac
     1441 gccagccttt gagacccggt tgcgggccat ccaggccagc ccggcgttgt cggtttcggt
     1501 gcggcccgac tggcagtttg atgccggact ggggcagcgc acgctgctgg cgcatttgca
     1561 ggccgccagt ctggccgcct ggcaggcgca ggacctggcg cacgcccatg ccgctgccgc
     1621 tgcgctgctc agttatgccg agcacaccca gggccgctcg ctgacccaca ttcagagcgt
     1681 gcgggtgcag cgcgccgacg atctgattga cctgccgcaa accacccggc gcaatctgga
     1741 gctgacacag accctgcgcg gtgaagacgc acccacgctg ttttcactgc tcgacacctg
     1801 catgaccggc atgggcagcc gtttgttcaa aagctggctg ctgtcgccca gccgcgaccg
     1861 caccgaggcc actgcccggc accgcgccat tgaggcgctg cgcagcggtg ccgccatgtc
     1921 ggctggcgtt ggcccctggg ccagattgcg cgaacagatc aagggcagca ccgacgtgga
     1981 acgcatcacc gcccgcattg ccttgcgcca ggtgcgcccg cgtgagctgg ttgggctgca
     2041 cgcgacacta caaaaaacag agctgctagc gcccgtcctg tatgggctgg atgcctattt
     2101 gacacaaatt tcagagcaac tgcagtcgcc tgcgggctgc gccgaactgc tgcgccaggc
     2161 gattgctgcc gagcccgctg cgctggtgcg cgacggcggt gtcatcgcca gcggctattg
     2221 cgccgagctt gacgagttgc gcgccatcca gaccaattgc gacggctttc tgctggacct
     2281 ggaaggccgc gaaaggctgc gcaccggcat tgccaacttg cgcgtgcaat acaacaaggt
     2341 acacggtttt ttcatcgaag tcacgcaagg ccagatcgac aaggtgccga ccgactaccg
     2401 gcgccgccag accctgaaaa atgccgagcg cttcatcacc cccgaactca aagcctttga
     2461 agacaaggcg ctgagcgccc aggagcgtgc gctggcgcgt gaaaaatggc tgtatgacca
     2521 ggtgctggac cagttgcagg cccatgtacc ggcgctggcc cggttggcgc gcgcactggc
     2581 cgcgctcgat gtgctgtgcg cgctggccga gcgcagcttg acgctggact ggaacgcgcc
     2641 gcagtttgtc aaagagccct gcattgacat cgaggctggc cgccacccgg tggtgcaggc
     2701 gcggctggcc gaactgagca acggcaactt catcgccaac gacacccgca tgggcccaag
     2761 gcagcgcatg cagatcatca ccggccccaa catgggcggc aaatcgacct acatgcgcca
     2821 gattgcggtg atcgtgctgc tggccagcat gggcagctac gtgcccgcca aaagctgccg
     2881 cctcgggccg cttgatgcca tccacacccg catcggtgct gccgacgatc tggccaacgc
     2941 gcaatcgacc ttcatgcttg agatgctgga ggccgcgcaa atcctcaacg ccgccacgcc
     3001 caattcgctg gtgctgatgg acgaaatagg acgcggcacc agcacttttg acggcctggc
     3061 attggccagc gcgattgcca cccagttgca cgacaagacc caggcctaca cgctgtttgc
     3121 cacgcattat ttcgagttga ccgagtttcc ggccacccac cacgctgcca tcaacgtgca
     3181 tgtgagcgcg gcagaatcag gccgcgacat tgtttttttg catgaaatcc agagcggccc
     3241 ggccagcaaa agctacggca ttgcggtggc gcggctggct ggcatgcccg ccgctgtgct
     3301 caaacacgcc cgccacacgc ttgaagcgct ggaacttcaa gccaaccagc atcaggccca
     3361 ggtggacctg tttgctgttc cacaagctac agaagttgta gctgtcagcg cagtagagac
     3421 atgggctaga gccctaaatc ctgatgattt gagcccacgc gaggcattgg aagcgctata
     3481 ccagttgaag aagctcagcg cggcaagtta gcgtgaaaca acggcatcat ggcaatctat
     3541 ccgtcaattt tggggcagcg gggtgtgcca aagatgccgc ttcatgctgt ggccctgcac
     3601 ggcggccaag gcgtgcttag gatgtggtca gaaataactt cccctatttg caccggcgga
     3661 tttgggcgca cagcgtcgtt gcaagacgct tgtttagcca gctaaacggc gcgtcttgcg
     3721 cctagccgtg catcccaact ccttggtgca aaacggggag ttattcccga ccacatcctt
     3781 agcggcaatc gttggatgca gcatggcatc catttttcat cgaacggccc agcaaaaaca
     3841 tgactttgcg atgcattcag gggcaaatgc ccggtttctt tttctagcca agtcaaggca
     3901 tccccgctag aatttttttg cgcatatcaa aagcgccgtg gctgaagcaa catgcatgct
     3961 gcttttccgc agtcacgcaa aaccaaccga aggaactgac agtgaaaaca tcaatcatca
     4021 agtggatggc agttgcggcc gtcatggtaa gcggccaagc ttttgcgggt gccgacgacg
     4081 acgcatggat tgccaagtgc gtcaaggaca acgccaagga gggtgccaag gcggacgttg
     4141 tcaaaaaata ctgcacctgc atgaacagca agatggacga caacgaaacc aagtccatta
     4201 cccaatggga aaagacgcat ccggatgaaa tgaaggcttg tgaaaaggtc gcgggctgga
     4261 aatagtcctg cttttgcaag gctttgacct gcgggctggt tcgtgcagac gagcagcccg
     4321 cttttttttg accacgtttt acctttggat cattcgcgtc tgggcatcaa gccgcagaca
     4381 ttgcaagcgc aggagagata acagcagggc aaatttgttc tttcgctaag tcgttgattt
     4441 accgtcattc ccgtgcaggc gggaatctgg tgcagtctgg attcccgcct gcgcgggaat
     4501 gccgaagatg ccacaagaac aaatttaccc tggcgatgac agcgaccagg acggtttgga
     4561 aatggcacga gttgccaaac tcagcgcagc gccccatgat gtcaaaaaat gcccggattc
     4621 aatcccggca ccccaccatt gctgcgttga acgcccatgt gggggcgacg caatcaatcg
     4681 gccaaacctg atcaatcacc aaaaaaaaca acaccgccgc caccgtttca gcgcaagcgc
     4741 acctcagttg cgccatgcat gctatcggtt ttcatgctct gcagcccagg cccaggccca
     4801 ggcttcgtcg gccagtgcgg tgacctgcgc aacaccacca agccgggcaa ccagttcatt
     4861 ggcctcatcg agctgatgcg attcaacctg ccagacagtt tgcagataga caaaccacgg
     4921 gccgctttgg tccagcaggg cttgttgggc cgcactgggt aggttcaggg tgctcaccac
     4981 gtcctgcggg ctgattttca ggatcaggct gagcattgat gccagcccga gcgtgaacaa
     5041 ctggtctggt gccggttcgt ggctttcctg cgccagacgt tccagcagcc gcgcgcgcca
     5101 cagggtcact tcctgcaagg cagaagagac cttgcgacga ccggcaaacc gcatcagcag
     5161 catagacagc cagcggtaca gtgcgttgcg acccagcaac agcaccgcct gatcaatgct
     5221 ggcatcagcc ttgatctggg caaaactggc gctgttgatg cgcttgagca aatcgtagga
     5281 caagccaaca tcgctcttga tgccgctgac aatcagcgcc gtgtcgctgc catcgtcgag
     5341 ctgatgcagc aggtgcccaa cccggtgaac ttcgggtgga agcggcaaca gacctcgcgc
     5401 ggcgctggtt atgccagccg gtgccagcac gccgcaggcg aagtggacac catggcacag
     5461 ggccatttca aggtcctcga catcggctac ttcggtgacc acgatgggca ggttttgcag
     5521 ctccgcaggc cagctttgtt ggcgctgcag cagtttggcc atgggctctg agcctgccgt
     5581 cagcagcgca aaatcaggcg tcaggtccca ggcgcaggcg gggtcccagc ccaccttggc
     5641 accggcagtg cgcaactgtt gcaccagttc ccgggcggct ggcaccagca ggttttcatg
     5701 atgttgctcg gtggccggtt caatgccaac ccagacccca gccgatactt ccaccgtaac
     5761 ggcatgcgcc agccagtcga ctggcacccg ggccagtccg gcgcgcccgc tggcggccag
     5821 cagacgcgcc gatgtcagca gcgcagccac atgcgcgccc cggccaagag aatcggcacg
     5881 atggcccagg cgatccagaa tgtgatcatt gatgtgaaat tcaaaaccgg caatggcgcc
     5941 gtctgaatca atcaaaggtc ggcgcgggcc ggtgatctgc acctcaggga cggtaccggc
     6001 ctgcgggtcc gggggattgg ccagagcacc tgcggcctcg gttgccgact gtgccggtgc
     6061 tgcaccaaag agtcgcggca gcaagtgttg caaaaatgtt ttcataccag cacctttcag
     6121 gccctgtggc atcaggacga atgggacatc gaacgcatca acctgggctc aatcacaagt
     6181 cacaagtatg cacctgcgaa atgcctgatc catccagact gatgccgggc acaatggtgt
     6241 tgcctgctgc ccggtgctgc ctcgtgctgc tttgagttgc gtgtccgggt atcgctgcat
     6301 gccagccaca cgacatctgg ctacatgacg atgcagaagt gaaacaggtc accggcgctc
     6361 tcggcagccg ccagcttggc ttcgcaatcg gccagttctt cgagcagttc aggcctgatt
     6421 tgttccttga tgcctttagc cgggccagct ttcaggcttt caatgattgt tctgagggcg
     6481 ggaagagcct gcgaggcttg aaaccacttt tcgttttcag caatccagga gtctggcagg
     6541 tcgtcttcgg acaggttgaa ttcaaggtca gttgtgtcaa agaagtccga cagcttgcgg
     6601 atggccagag cgtctgatag cttgtcgagc gcctgcgagt gcttgcaaaa aagggagtgg
     6661 tcgtagtcgt cgccagcatc gtcagtctgc gcgagggact ttcttctgac ccaaatcgtg
     6721 tcgctcattg gctgctccgt agaggtgtga tcagcgggct tgccagccca aaaaatcaag
     6781 gaacgctgat catagccgca gcagcagcct ggctcgctga ctggcaaagg cagcacaaaa
     6841 tgctagcgga tttgcgcaat gtcggccaga aactgcacat atattttgcc cagtgcctgt
     6901 tccatcgcga cgacaaatgt gccgattgat tcgtcgctgg caggctgctc tgcctcaaag
     6961 gtcctggtca acaaattgac agcagcgcgt tccaggcgca gttcgatggc caccaccgcc
     7021 ttgctgtt
//
LOCUS       DAOSLL010000180         7016 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9760_length_7016_cov_3.241488, whole genome shotgun sequence.
ACCESSION   DAOSLL010000180 DAOSLL010000000
VERSION     DAOSLL010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 7016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7016
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9760_length_7016_cov_3.241488"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(400..810)
                     /locus_tag="PLB25_09425"
     CDS             complement(400..810)
                     /locus_tag="PLB25_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41832.1"
                     /translation="MANSESGLIPALETALQAATTALDCAQLFDMPEIKAHAASVTRV
                     SDYLGNLWRRGLVERLRGAKKADGRTRWMYQWRGNRMPSAESIMYSPKVLTDRPSMLI
                     TEEGGIMTIEMANLVISIRQKPTGSSYLKSLTGT"
     gene            complement(1109..2065)
                     /locus_tag="PLB25_09430"
     CDS             complement(1109..2065)
                     /locus_tag="PLB25_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41833.1"
                     /translation="MSLHIGTPQANTSEVEFPEARLIWQGQGCDWLGAIEAIKPDPTA
                     GHYARPMDRIPLRSGQLRCDIEDCEELLELEDRQIIRWKFTSMGVAPDVGLLSKDELE
                     LSEVVDRYCGRKTRLLLPHLIAYLRQNGGWTKPYLRANFNKAMIGIGGTKVLVGRPTV
                     PIRLLDSLPTSALLIVPHMSASGPNGIWTIERGEHHPLVLAASKLRVHALFEQDGQPE
                     FYCRASINGDAGLCDSDSAKGIDLFTSLRAARKWIKQGVEDGEPCLIAKLLTPHELLS
                     CLSRVDIASINLTDFAMTQRGKVALGHDKIAATNEALQALTR"
     gene            3302..3985
                     /locus_tag="PLB25_09435"
     CDS             3302..3985
                     /locus_tag="PLB25_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE41834.1"
                     /translation="MSNQILLGKRVLITHADAFMGPVLCEVFANHGATVIPSTDHLLS
                     TDALVALVAEAGHLDILIANLAIPAPTTVATEVSDKEWKDTFDALVHPLLRLFRAGLP
                     AMIKRRAGKILVMGSASALRGMKRTSTYSAARGAQLAYVQAVGVEVAHHNVQVNAIAQ
                     NFVDNPTYFPAELQSDPRFQERLKREVPLGRLVGATEDAEFAAYLCSEPASCFVGQVF
                     PVCGGWVGR"
     gene            4373..4576
                     /locus_tag="PLB25_09440"
     CDS             4373..4576
                     /locus_tag="PLB25_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41835.1"
                     /translation="MPGSWKSKFLIWGADQPTFAGGSNVPIAVNRKVEELSFASMAMK
                     PKVVTQILVSALRNLLVPAQAVN"
     gene            complement(4656..5795)
                     /locus_tag="PLB25_09445"
     CDS             complement(4656..5795)
                     /locus_tag="PLB25_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOE41836.1"
                     /translation="MTTPKRETTGTEEPLSPVPIQTGSLSLVVLASLACIYTLHWASA
                     VFIPVMVGMLFSYALSPIVDWLQVRCIPRAVSAAALVLGIVASIGLGVYSLGDEASKL
                     VDLLPQAAQKLNSIVRTSGGGADNTFNTVQKAAARLEQAATDSTAPASLSRGVQRVQV
                     EKSRFDIKDYFWSGTVGLFQMLGQAAMVALITFFLIASGDNFRRKVVKLAGPSLHSKR
                     ITLQALDQIHEQIQRYMLVQLFTSVLVGLATWLCFLAIGLENAAVWGVAAGLLDLVPY
                     VGSVVIAGASALIGFLQFSTLEMALLVSGASLTIHFVEAFLLTPWLTSRASKMSPVSI
                     FVGVLAWGWLWGIWGLLLGVPILVIIKSICDRTEGFKSVGELLGA"
     gene            6269..>6791
                     /locus_tag="PLB25_09450"
     CDS             6269..>6791
                     /locus_tag="PLB25_09450"
                     /inference="COORDINATES: protein motif:HMM:NF025631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4360 domain-containing protein"
                     /protein_id="HOE41837.1"
                     /translation="MKRNVFIVAAMVISAPAFAVDGITLGEPGYGGTGCPAGSVSATL
                     SPDATSLSLLFDKYQVAVGGSTGKSFDRKSCNIAIPVRVPQGFSVSVLKIDYRGFNQL
                     PQAASSQFNVEYFFAGTRGPAFQRKFAGPLQSDYLINNELTVQSIVWSACGADVNLRT
                     NSSMRVQTVANREA"
     assembly_gap    6792..6801
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1648 a         1911 c         1863 g         1584 t
ORIGIN      
        1 cgtaggcttt tagtgtacgc tgttggacaa tcaaaggcta tttttttgaa taaatgtctc
       61 gtaaatagtt gatttataaa gactgtcgat ttttaagttt cttatgaaat ttcattggaa
      121 actaaatttc ttcgttaggg acgaaggggt aggatgttcg aatcctctca ttccgaccat
      181 ataaatcaac gggttagcag attttgattg ctaacccgtt tttcgtttgt gttgtgtgaa
      241 ttgtatttac ccaccacttt acccaccagt tagacgagtg ctgtagaaat atcagtcagg
      301 cacttcgcag ctgactgaat ccgactttca tagcgcggtg ctgcagcggt ttcattgaag
      361 gcgaaagggc agcccgtgaa gaaccgcacc atcaagaact caagtaccag tcaatgattt
      421 caagtagctc gatccagtcg gtttctgtcg tatcgaaatg acaagattcg ccatctcgat
      481 cgtcataatt ccgccttcct ccgtgatcag catggacggt ctgtcggtca gcactttcgg
      541 gctgtacatg atgctctcag cgctcggcat acggtttccc cgccactgat acatccaacg
      601 tgtgcgaccg tcagctttct ttgctccacg aagccgctcc accagacccc ggcgccatag
      661 gttacccaaa tagtcggata cacgagtgac gctcgcagcg tgtgccttga tttcaggcat
      721 gtcgaataat tgtgcacagt caagcgccgt ggtagcagct tgcaatgcgg tttcgagggc
      781 ggggatcagc ccagattcag aattggccat ggtggcctcc atatatgtgt tgtttaacga
      841 gggaatgttg cccaccggct cttcaacggg ttaccggtga tgagcggttg ggacgcagga
      901 tcgatttcag atttgatttg caggtgctct ctctcctttc gatggtgaaa cttcaatggt
      961 tgacgtcgat ttggttgata ggcatggcga tgccaaggcg tgacaccgcc cgccttcgca
     1021 tcacgatgcg gtgaattcac ggccagggaa ctgatgcggc aggcggatgg agtcaactgc
     1081 ccgatgtgcg cccacaggtg gcgcaggact accttgtcag ggcctgcagc gcctcatttg
     1141 ttgcagcgat cttgtcgtgc ccaagcgcca ccttgccgcg ctgtgtcatt gcgaaatcgg
     1201 tcaggttgat cgaggcgatg tccacccgcg acaggcagga caacagttcg tgcggggtca
     1261 gcagtttggc gatcaaacac ggctcaccgt cctcgacccc ctgtttgatc cactttcttg
     1321 ctgcccgcaa cgaggtgaac aggtctattc ccttcgccga gtctgaatcg cacaagccag
     1381 cgtcgccgtt tatgctggcg cgacaataga actcaggttg accgtcctgc tcaaacagcg
     1441 cgtggacccg cagcttgctg gcagccagca ccagcgggtg gtgctcacca cgctcaatgg
     1501 tccagatgcc attgggccca cttgctgaca tgtgaggcac gatcagcagc gcagaagtcg
     1561 gcaggctatc aagcaaacgt attggcaccg tgggacgacc caccagcacc ttcgtgccac
     1621 cgatcccgat catggccttg ttgaaattgg ctctcagata gggctttgtc cagccaccat
     1681 tctggcgcag gtaagcaatc aggtgaggca gcagcagacg cgtcttgcgt ccgcaatagc
     1741 gatcaacaac ctcggacaat tccagctcat ccttgctgag cagcccaaca tccggtgcaa
     1801 cgcccattga cgtgaatttc caacgaatga tctggcggtc ttccagttcg aggagttctt
     1861 cacaatcctc aatgtcacac cgcaattgac cggaccgcag tggtatccga tccatcgggc
     1921 gagcgtagtg cccggctgtg gggtcaggct tgatcgcctc aatggcacca agccagtcgc
     1981 aaccttggcc ctgccagatc agcctggctt ctggaaactc cacttcagag gtattggcct
     2041 ggggcgtacc gatgtgcaag ctcacactct ttgcgccttg aaatagctct gttgcgacca
     2101 tgcgctcggg tggtggaagc ctatcttcat tcacaatctc gaccagcagg tccaagtcac
     2161 cacaatcggg agcacctcgg gcatacgacc cgaccgccca tagtgaagtg actgtgaacc
     2221 ggctagtgca gtcgacttcg ataatcgggt atttccagtg gatcaaccgt tgcggtttga
     2281 cgtccaggcg ctggcatatg gcagccagcg ttgcggtgaa tgatgaacgg gggcgacgag
     2341 ttgaggtcat gatggttctt cagtaaaagg ttgtgaaaga tggaaccggt tctgccgatt
     2401 caggcagtcc tgtgacatgt gccgctgaca ccgttgggca ctcaggacgc agagatgcaa
     2461 agcgcagacc ggcagcgcta agacacctgc ctagcgcacc tgcgcagcag gggcaagaca
     2521 taatgaaaac aaagaaatga acaccagcgg atgcctgatg caccgctctg cgagcaacac
     2581 acagagagca gtcaaaacgc aagaacaaga cggcgaccac gtcgccgtga gctgcgctac
     2641 agcgaccgaa acaaaaggga tgagtgatac agcggctgta cccagccgct gcagggctaa
     2701 gcagtgaacc ttagaaggtc aagctgacgc gatgactgat gcagccgagg aacgaggctg
     2761 cagggagtta gggtcccctt ttggctgggc aggaagtcgg atcccgaagt gccagtggct
     2821 tcaattgcac ccgggggaag ggacccgtgc ggacgtatct ggacgattta agtcgaaagt
     2881 ggcttttatt ttgcagcaaa attgatgcct aattcgcctc gcgcgccgcc ttggtccaga
     2941 cgttaaaagg gacaatttac taatagataa tctatttgta aattgtccct ttttgatttc
     3001 acgctcacct tgttcggccg gccgcaggtg tgggtcgtta cagtatcacc tggctttcag
     3061 tgcctcgtct tgtccgattg ggcctggatt tgtagcttaa tggaagatgc ccccgacgca
     3121 ctgcacagcc aaacttcacg gtcaccgtgc tgctaggcca ttgatcccca tcttgacggg
     3181 gttgtcgtta gatcgtaggt taagcccggc ctgcagcgct acgttcgctg ctgatgccac
     3241 aagttgagtg tcttgataga ctgtgacttg ccgtcacttc tcgttttcca aaggagagtc
     3301 tgtgtcaaat caaatccttc tcggcaagcg ggtgctcatc acccacgccg acgcgtttat
     3361 gggcccggtc ctttgcgaag tgttcgccaa tcacggtgcc acggttatcc cgagtaccga
     3421 ccatctcctg agcacagatg cgctcgtcgc tctcgtcgcc gaggcagggc acctcgatat
     3481 cctcattgcc aacttggcaa taccagcacc gaccactgtg gcaacggaag tttcagacaa
     3541 agagtggaag gacacttttg acgctctcgt acatcccctt ttgcgattgt tccgtgctgg
     3601 cttgcccgcc atgatcaagc ggcgcgcggg caagattctc gtcatgggca gcgcatcagc
     3661 actgcgcggg atgaagcgga cctcgaccta cagcgctgcg cgcggagccc agcttgccta
     3721 cgtgcaggct gtcggcgtgg aggtcgccca tcacaatgtt caagtgaatg caatcgcgca
     3781 gaacttcgtt gataacccaa cctactttcc agccgagctc cagagtgatc cacggttcca
     3841 ggagcgccta aagcgtgagg ttcctctggg acgcctcgta ggcgcaacag aggacgccga
     3901 atttgctgct tacctttgta gcgaaccggc gagttgcttt gtagggcaag tgtttccggt
     3961 ttgcggcggc tgggttgggc gctagtgcaa gtgcagccca ccccgttatt ccaccggact
     4021 tacgcgaaaa gccacccaaa ctgagtgtga tcaacgaatt tgatggccta ttggtcacat
     4081 tcggtgatgg gcaccattgg cagcgaatat catcggtttg tcgtactgcc acaacaggct
     4141 tgaccgtcaa tatcagtgac tgcttcagag tcagatctac ttcgcctgtg cagcggaagc
     4201 ggacggtcgc agtcaaccct ggaattgcat cgatttctcc aaagcggtca ttgccgatgc
     4261 cggtccaatt tcgtcatttg ttcacctcca aaactcgaaa gcggacatac gagggaaacg
     4321 atcagtttct gccagtggct gtcggtactg cagcggttgc tgtcggtcac caatgcccgg
     4381 ttcctggaag tccaaattct tgatttgggg cgccgaccag ccgacgtttg cgggcggctc
     4441 caacgtgccc attgcggtca accgtaaagt tgaagaacta tcgttcgcct cgatggccat
     4501 gaaacccaaa gtagtcacgc agattttggt ttcagctttg agaaatcttt tggttccagc
     4561 gcaagctgtc aattgatcat tgcacgatga tggcaaagtt tatggataca ttggactaac
     4621 gaaaatcgtt caatattgaa ccaatcgggc ccgcgctagg cacctagcaa ttcgccgact
     4681 gatttgaaac cctcggtacg atcacaaatg gacttgatga tcaccagaat cggcacaccc
     4741 agcagcaaac cccaaattcc ccagagccag ccccaagcta gcacaccgac aaatatggac
     4801 acagggctca ttttgctggc acgactggtt aaccaaggtg taagcaagaa tgcttccacg
     4861 aagtgaatgg tcagtgacgc tccgctgacc agcagggcca tttccagcgt actgaactgc
     4921 agaaagccga tcagggcgga ggcaccggca atcaccacgc tgcccacata tggaaccaga
     4981 tccagcaaac cggcagcgac accccagacc gcagcattct ccagcccaat ggccaaaaag
     5041 cacaaccagg tggccaatcc caccagcacg ctggtgaaca actggaccag catatagcgc
     5101 tgaatctgtt cgtggatctg gtccagcgcc tgcagcgtga tacgtttact atgcagagag
     5161 ggaccagcca acttgacgac tttgcgccgg aagttgtcgc ctgacgcaat caggaagaac
     5221 gtaatcagtg ccaccatcgc cgcctgcccc agcatttgaa acagtccaac cgtgccgctc
     5281 cagaaatagt ccttgatgtc aaatctggac ttttccacct gtacccgctg caccccacgg
     5341 ctcagcgatg ccggcgcagt ggaatctgtc gcagcctgct ccagccgggc tgcggctttt
     5401 tgtaccgtat tgaaggtgtt gtcggcgcca ccgccagaag tgcggacgat cgagttgagt
     5461 ttttgcgcag cctggggcaa taaatcaacc aacttgctgg cttcgtcacc caatgagtag
     5521 accccaagcc ctatgcttgc tacgatgccc agaaccaacg ctgctgcact gaccgcacgt
     5581 ggaatgcatc gcacttgcag ccagtccacg atgggcgaga gcgcataact gaaaagcatg
     5641 ccgaccatca ccggaataaa aacagcgctg gcccagtgca aggtgtagat acatgccagg
     5701 gatgccagaa ctaccaggga aaggctgccg gtttgaatgg gaactggcga cagtggctcc
     5761 tcggtgccgg ttgtctcgcg ctttggcgtc gtcacggctt gatacctccc gaattcacac
     5821 caaagctctg gaatttgtgg acaacggtct ggcacagatg ttttgagccc aacataaggt
     5881 ggcaataccc gttcgctacg ccgaggtcgg cgccgaaaac ggcaggttgc gcgttgatca
     5941 agaatttaat cgatgtcatt ggaaaagccg atttcaggac caaggagtgg gtataaagtg
     6001 gataaacctt accagtcaac tttggcctgt cctggaatag acatctgttc ggtagcgcac
     6061 aacgacggcg gtcggcccgc gagtcactcg gaaatcggca aatattgcgc aggctcgctt
     6121 ttcggttgtg tgcgccagta gacatacgac caagactatt caccataagc ttctatcaac
     6181 caagccaaca tgctcggttg cgaggtctac acaaaggcga tccgttttga gttgatcaag
     6241 ttcattgact accaacagga gtttcaacat gaaaagaaac gtttttatag tcgccgccat
     6301 ggtgataagt gcaccggcat tcgccgttga cggtattacc ttgggcgagc ccggctacgg
     6361 cggaactgga tgtcctgccg gctcggtatc cgccacattg agtccagatg caacttcttt
     6421 gagtttgcta ttcgacaaat accaagtagc cgttggtggt tcaactggaa aaagctttga
     6481 ccgtaagagc tgcaacattg ccattcctgt gcgggtgccc caaggattca gtgtatctgt
     6541 cctgaaaatt gactaccgtg gctttaatca actgccgcaa gcggcaagct cacagttcaa
     6601 tgtcgaatac ttcttcgcgg gtaccagagg accggcattc caaagaaaat ttgctggccc
     6661 actgcaatcg gactatttga tcaacaacga gctcaccgtc caatcgatcg tctggtcagc
     6721 ttgcggcgcg gacgttaatc ttcggaccaa ctcaagcatg agagtgcaaa cggttgctaa
     6781 tcgagaagcc annnnnnnnn ngacacccaa gacgtcagtg cgggcatcat ttaccaattg
     6841 caatggcgtc cgtgcacata gatgttaata agccacattg gaaatccatc atcgccatat
     6901 ttccagcgga atattgaagg ttgaattgag aagagcagct atgaagttga taacaaaatg
     6961 tattttctta gtcgcgtgcc tagcctaccc gcctgcgtgg gcccaagaga ccaacg
//
LOCUS       DAOSLL010000181         6981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9824_length_6981_cov_3.976465, whole genome shotgun sequence.
ACCESSION   DAOSLL010000181 DAOSLL010000000
VERSION     DAOSLL010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9824_length_6981_cov_3.976465"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            571..942
                     /locus_tag="PLB25_09455"
     CDS             571..942
                     /locus_tag="PLB25_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41838.1"
                     /translation="MAQLTPLSKGLLGLLVIGGMASAAWHLGLKERFGAMLGSAAPPV
                     LSAPDSAASEVSAPPAAVAPVAVTPAAAQPERLEQRVSERQQPAPIVNHPQPQAGTSK
                     SSSARADPWAELKIDKKGNSP"
     gene            939..1880
                     /locus_tag="PLB25_09460"
     CDS             939..1880
                     /locus_tag="PLB25_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41839.1"
                     /translation="MNISALVAGLLLFGSAAAQAEGDFTVSTGPGSGVYTQAVANMQE
                     VCPDAEIVEVKSSGAPENLENCLLGKVTACFVNADQMWGAKNIEGRDAEVENIKTILP
                     FYDNAIHMISRNPGIVNFSDFNAKVIGTFGGAAITLRVMLAKSERGGAPVRPAKVVNF
                     DREKDPQKAMLEAWGAGKVDAIVGIGYAPIPWVEKINAQDAHLVTYDRWNDVKDLSIQ
                     NGGFYRMKTIGYKNLDGNASTLTVRTILASARNWPANAPETKQIQKLFVCMTKDSTRQ
                     TLKSGAGRHFHAFWITYSPMTDPAWPWYGNLRPTGKK"
     gene            complement(2046..2351)
                     /locus_tag="PLB25_09465"
     CDS             complement(2046..2351)
                     /locus_tag="PLB25_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41840.1"
                     /translation="MDFNETDEKFSDQVKQADQPASPSQQTSNQMQQSLAQASPSRLG
                     IREQVGSMTQRGRDILWQTSQHLQLDAGHTSENTLNPHQHAASLPMTAAARMRHLAG"
     gene            2636..2935
                     /locus_tag="PLB25_09470"
     CDS             2636..2935
                     /locus_tag="PLB25_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41841.1"
                     /translation="MINSERLGGWVLKFGFSTGTSMMASCSASRRNALRRASRRVNLT
                     HVENKFSSSLPGSKSLIAEADRLEAGGFNLVMDIKRETATVTAELNHSNPTVRAS"
     gene            2971..3348
                     /locus_tag="PLB25_09475"
     CDS             2971..3348
                     /locus_tag="PLB25_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41842.1"
                     /translation="MSALSPLLATLAAAGQRALRALMALTCALHCASTALAAGPTGLL
                     NDTGQTNCYDGSAMVACSGANTGNAAAYPGQDARFGRDRATPPKVGGGAAGFDFTKVC
                     MNGTLNCTGAANTGDSPAASEWA"
     gene            3317..3529
                     /locus_tag="PLB25_09480"
     CDS             3317..3529
                     /locus_tag="PLB25_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41843.1"
                     /translation="MTALLPASGLDGQQQHRHSGRAGVGGRGKLRLHRQASNGTYSSA
                     ATAAASAIPEPKRKSDLTPILMLLLD"
     gene            3813..6611
                     /locus_tag="PLB25_09485"
     CDS             3813..6611
                     /locus_tag="PLB25_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOE41844.1"
                     /translation="MSQTLNAAASQGGKPATNEVFHAMAVADVIAKLQTDLETGLTTG
                     EANARLAQYGPNVLPKAKGDTPWSLLWRQINSPLIWVLVASGFVAMLVDPTDGIKNGL
                     VILGVVVLNTLIGFVQEFKAGKAIEALSKMVPENVTALRDGNKATVAGAELVPGDVIL
                     LASGDKVPADLRLAAVRTLKIEEAALTGESVPAEKSTEASGANAGIGDRSGMAFGGTL
                     VTYGTATAVVCGTGATTELGRISTMLKETTNLQTPLTKALQSIGKYITIGIVVIAVIM
                     LVVGTWRAMAETGIAFLPALRETVIFAIALAVGAIPEGLPAIVTIALAIGVQRMAARR
                     AVIRKLPAVETLGSTTTICSDKTGTLTRNEMTVQALWTTRGSYAITGVGYEPAGQLKD
                     SCGIDLSENVPTDVRDLLLAGALCNDATLVHEGDTYIITGDPTEAALVVSAEKIGVKV
                     ENARRDHARLDAIPFESENQFMATLNKHPDANPRIILKGAPEVALKRCQGIEPAPVLA
                     EVSRLAGQGMRVLAIASKAAAQHGQLQMPDTSGGFTLLGLQGMIDPPRSEAIEAIKAC
                     HKAGINVKMITGDHRETAQAIGAQLNILTDAEAVTGAQLAEMSEEQLRQVVRTTNVFA
                     RVAPEHKLKLVRALQANHQVVAMTGDGVNDAPALKQSNIGVAMGITGTAVSKEAADIV
                     LTDDNFASIAAAVEEGRRVYDNLIKSLVFILPTNLGLAMILMAAVIFFPFLLNPATGL
                     KELLLPMAPTQLLWINLVATVALALPLAFEAKEPNVMRRPPRSPDAPVLSGFVIMRTF
                     VAATLMTIGAVGLFLWEYNTNGETLDAMKKGQTMAVTTVIMFQVFYVLDCRSIKNSLL
                     SIGLWSNMAVFIGIAALLLLQLGFIYLPFMNLIFGSLPLSAKDLLLATAVGMIILPVI
                     SVEKLLRNRIEAGSAQKR"
BASE COUNT         1456 a         2099 c         2100 g         1326 t
ORIGIN      
        1 atgcgcagcc aatggcggcc tgaacacgaa ggggggaact acgcactgtc cagttcccag
       61 ggtgccgtct tgaatcgcca atcgttgttt gacgagaggc accatcggtg cccaggctgc
      121 ggtgccaaag ccagccatga aaaaagcgat gagtgttgca cttgcttcgg ctggtccagg
      181 caaaaggccg gtttgttgtg atgctggtca ttgcgccttt tttagcggcc ccttgctgtt
      241 cgcaagggcc atcggctgat tttatgcaca gccggtcaac accgccgatc agtcaagcgc
      301 ctgacggcct gccgcgcctg aacaggctcg cacaaaatgc gctggttgga tgcctggtag
      361 caattggcaa accaccgcag gggtttcgca tgggggatgt gcagagtctt gtgcgcaaca
      421 ttggatatag tgcgaacagc gcgcaatgac ttgcgcggct gctggcccgc tacgctacag
      481 cttgcaggct gtttggaact aaccgtggag ttgtcaatga agggcatgat gagtattttt
      541 gagacaccgg cttgggtgga gggctagacg atggcgcaat tgaccccgtt gtccaagggc
      601 ctgcttggct tgctggtgat tggtggcatg gcctcggccg cctggcacct tgggctcaag
      661 gagcgatttg gtgcgatgct gggcagcgca gcgccgccgg tgctgagcgc gccggattcg
      721 gctgcgagcg aggtttcagc gccacccgct gctgtggcac ctgttgctgt gacacccgcc
      781 gcggcacaac ccgaaaggct ggaacagcgg gtaagcgaac ggcaacaacc tgcgcccatc
      841 gtgaaccacc cgcaaccgca agctggtacc tcaaagtcaa gctcggcacg cgccgatcct
      901 tgggctgaac tgaaaatcga caagaagggg aattcaccat gaacataagc gcactcgtcg
      961 ccggtttact gctttttgga tcagccgcag cgcaggccga aggcgatttc actgtctcga
     1021 ccggccctgg gtcgggtgtt tatacgcaag ccgtggcgaa catgcaggaa gtctgtcctg
     1081 atgccgaaat cgttgaagtc aagagcagcg gtgcaccgga aaatctggaa aactgcctgc
     1141 tcggcaaggt cacagcctgt tttgtcaatg ccgaccagat gtggggggca aaaaatatcg
     1201 aagggcgtga cgccgaggtc gagaacatca agaccatttt gccgttctac gacaacgcca
     1261 tccacatgat cagccgcaac cccggcatcg tcaacttctc cgatttcaac gccaaggtga
     1321 ttggcacgtt cggtggcgca gcgatcacgc tgcgggtgat gctggccaaa agcgaacgtg
     1381 gcggcgcgcc ggtgcgccca gccaaggtgg tcaacttcga ccgtgagaaa gacccgcaga
     1441 aagcaatgct cgaagcctgg ggtgccggca aggttgatgc gattgtcggc atcggctatg
     1501 cgccaattcc ctgggtcgag aaaatcaacg cccaggatgc ccacctggtg acttacgacc
     1561 gctggaatga tgtgaaggac ctgagcatcc agaatggtgg cttctaccgc atgaagacca
     1621 tcggctacaa aaaccttgac ggtaacgcct caacgctgac ggtgcgcacc atcctggcct
     1681 cggcgcgaaa ctggcctgcc aatgcacccg agaccaagca gatccagaag ctgtttgtct
     1741 gcatgacgaa agacagcacg cgccagaccc tcaaaagtgg tgccggacgg catttccacg
     1801 cgttctggat cacttacagc ccgatgaccg atccggcctg gccgtggtac ggcaatctgc
     1861 gacccacagg caagaagtaa gccgacgggc tcggcacaaa gatcgctcgc gcgaccggtg
     1921 tggtggccac gccgccagcc ttggttcaga ccatttttta aggtaaatca gcctgcagac
     1981 cttatagggc aagcgctgaa tgctataaaa tatatagtcc ctgaagagcc tcgccgcctt
     2041 cctggttagc ctgcgaggtg gcgcattctg gcagcggcag tcattgggag actggcggcg
     2101 tgctggtgtg ggttcaaggt gttttctgaa gtgtggccag catcaagttg caaatgctgc
     2161 gatgtctgcc acaggatgtc cctgccacgc tgcgtcattg atccgacctg ttcacggatg
     2221 cccaagcgtg agggcgaggc ctgggcaagg ctctgctgca tttggttact cgtttgctgg
     2281 gagggcgaag caggctggtc agcctgcttg acttggtcgc tgaatttctc gtctgtttcg
     2341 ttgaaatcca tagtggttcc tgtaagctaa ttcaagaggg ttggcgcagt cgcctggaga
     2401 cctaccttgc atcacggcgt ttgtgcgctg gcgaacgata tgtggtcaac aatcgctggt
     2461 ctgtgtgctg gcacacacag gttcaatacc aggcaagccg tcgttgcagc gccaaaggcg
     2521 gcctatcgga gtggcgggaa ggcttgaccg cagaggttgc taccccggtc aactgctgat
     2581 accgtctgcg ccaggccttt ttcaggtcaa acaccgcgcc gaccacacca ggcaaatgat
     2641 caattcggaa aggctcggcg gctgggtctt gaagttcggg ttcagcaccg gcacatcaat
     2701 gatggccagt tgcagcgcct caagaagaaa tgccttgcgc cgcgccagcc gccgcgttaa
     2761 cttgactcac gtggaaaaca agttttcaag ttcgctgcca ggctccaaaa gtctcatagc
     2821 tgaagctgac cggctggaag ccggtggatt taaccttgtg atggacatta aaagggagac
     2881 agctactgtg actgccgagc taaaccattc gaacccaacc gtccgtgcta gttagcgccg
     2941 ggcggttttt tcatttcgga acctttgttc atgtctgcgc tatccccctt gcttgccaca
     3001 cttgctgccg cggggcagcg agctttgcgc gcattgatgg cgctgacctg cgccttgcac
     3061 tgcgccagca ccgccttggc cgctggcccc accggcctgc tcaatgacac cgggcaaacc
     3121 aattgctatg acggcagcgc catggttgcc tgcagcggag ccaacaccgg caacgccgcc
     3181 gcctaccccg gccaggatgc ccgttttggc cgggaccggg caacaccgcc aaaggtcggg
     3241 ggcggcgctg cgggctttga ctttaccaag gtatgcatga atggcacgct caattgcaca
     3301 ggcgcggcca acaccggtga cagccctgct gccagcgagt gggcttgacg gccagcagca
     3361 acaccgccac agtggtcgtg ccggggttgg tggccggggc aagctacgcc tgcaccgcca
     3421 ggcgagcaac gggacttaca gcagcgcggc gacagccgcc gcttcagcca taccggagcc
     3481 gaagcgcaag tccgatctga caccgatcct gatgctgctg ctggattgaa ttcagcgcaa
     3541 gttttcatgc acttctgcag tcggccccac agggttgact tgccagcacc gttggggcgt
     3601 gccagcaaat aagaaaatga gccagacgat cattccaggg tgagctgaac agtttgcaac
     3661 ggatgccagc gcctaagcac agcgcagggt ccgggcatct gccaagcaga tcagtcagag
     3721 aggcatcagt ttgaatatcg gatcgcctga aagatgccat acttggcttt cagtggttga
     3781 attttctgtc tgctaacgag gaaaaccggt caatgtcaca aacgctcaat gctgcggcaa
     3841 gccagggcgg caagcccgcg accaatgagg tgtttcacgc aatggcggtg gcggatgtca
     3901 tcgccaagct gcaaaccgac ctcgaaaccg gactgaccac cggcgaggcc aacgcccgac
     3961 tggcccagta cgggcccaat gtgctcccca aggccaaggg cgacacgcct tggtcgctgc
     4021 tctggcggca gatcaactcc ccattgattt gggtcctggt cgcttccggc ttcgtcgcaa
     4081 tgctggttga cccgaccgac ggcatcaaga acggtctggt catcctgggt gttgtggtgc
     4141 ttaacacgct catcggcttc gttcaggaat tcaaggcggg caaggctatc gaggcactca
     4201 gcaagatggt tcccgagaac gtcaccgcac tacgcgacgg caacaaggcc accgtggccg
     4261 gggccgaact cgtgcccggc gacgtgatcc tgttggcatc gggcgacaag gtccccgcgg
     4321 acctgcgact cgcggcggtc aggacgctca agatcgaaga ggcggccctc accggtgaat
     4381 cggtccccgc tgaaaaaagc accgaagcct caggcgccaa cgccggcatc ggcgaccgct
     4441 cgggcatggc atttggtgga accctggtga cctacggcac cgctaccgcc gttgtctgtg
     4501 gcaccggcgc gacaaccgaa ctgggtcgca tctcgaccat gctcaaggaa accaccaacc
     4561 tgcagacccc gctgaccaag gcactgcaaa gcatcggcaa gtacatcacc atcggcatcg
     4621 ttgtcatcgc ggtcatcatg ctggtcgtcg gcacctggcg tgccatggcc gagaccggca
     4681 tcgcgttcct gccggcgctg cgcgagaccg tcatctttgc catcgcgctg gcagtcggcg
     4741 cgatccccga aggcctgccc gccatcgtca ccatcgcgct cgcgatcggt gtgcagcgca
     4801 tggccgcgcg gcgcgccgtc attcgcaagc tgccggcggt cgaaacgctt ggctcgacca
     4861 ccaccatttg ctccgacaag accggcacgc tcacccgcaa tgaaatgacc gtgcaagccc
     4921 tttggaccac gcgcggcagc tacgccatca ccggtgtcgg ttacgagcct gccgggcaac
     4981 tcaaggattc ctgtggcatc gacctcagcg aaaacgtccc gaccgacgtc cgcgacctgc
     5041 tgctggccgg cgcgctgtgc aacgacgcga cgctggttca tgaaggcgac acctacatca
     5101 tcaccggcga cccgaccgag gcggcactgg tcgtctcggc cgaaaagatc ggcgtgaagg
     5161 tcgagaatgc gcggcgcgac cacgcccgcc tcgacgcgat tccgttcgag tcggaaaacc
     5221 agtttatggc cacgctgaac aagcatcccg atgccaaccc gaggatcatt ctgaaaggcg
     5281 cgccggaagt ggctctcaag cgctgccagg gcatcgaacc ggcccccgtg ctggcggagg
     5341 tctcgcgact ggcggggcag ggcatgcggg tgctcgcgat tgcgtccaag gcggcagcgc
     5401 agcacgggca gttgcagatg cccgacacgt ccggtgggtt cacactgctg ggcctgcagg
     5461 gcatgatcga cccaccgcgc agcgaagcca tcgaggccat caaggcgtgt cacaaagccg
     5521 gcatcaacgt caagatgatc accggcgacc acagggaaac tgcgcaggcg attggcgcgc
     5581 aattgaacat cctcaccgat gccgaggccg tcaccggcgc acaactcgcc gagatgagcg
     5641 aggagcagtt gcgccaggtg gtgcgcacga ccaacgtttt cgcccgcgtc gcgcccgagc
     5701 acaagctcaa gctcgtcagg gcgttgcagg ccaaccatca ggtcgtggca atgaccggcg
     5761 acggcgttaa cgacgcgccg gcgctcaagc agagcaacat cggcgtggcc atgggcatca
     5821 ccggcaccgc cgtctcgaag gaggcggcgg acatcgtgct caccgacgat aacttcgcct
     5881 ccatcgccgc cgccgtcgaa gaaggccgcc gcgtctacga caacctcatc aaatcgctcg
     5941 tcttcattct gccgaccaat ctcggcctgg cgatgatcct gatggcggcg gtcatcttct
     6001 ttccattcct gctcaatccc gcaaccgggc tgaaggagct gttgctgccg atggcaccga
     6061 cgcagttgct gtggatcaac ctcgtcgcga ccgtggccct ggcgctgcca ctggccttcg
     6121 aagccaagga gccgaacgtc atgcgacgac ctccacgcag cccggatgcg cctgtgttgt
     6181 cgggtttcgt catcatgcgg acgtttgtcg ctgccacgct gatgacgatc ggggcggtcg
     6241 ggctgttcct gtgggaatac aacaccaacg gtgagacttt ggatgccatg aagaaggggc
     6301 aaacgatggc tgtgacgacg gtcatcatgt tccaggtttt ctacgtcctc gactgccgca
     6361 gcatcaagaa ctcgctgctg tcgatcgggc tgtggagcaa catggccgtg ttcatcggca
     6421 tcgccgcgct gctgctgctg caactcggct ttatctacct gccgttcatg aacctgatct
     6481 tcggttcgct gccgctctcc gccaaggacc tgcttttggc gaccgctgtc ggcatgatca
     6541 tcctgccggt gatcagcgtc gagaagctgc ttcgcaaccg gatcgaagca ggttcggcgc
     6601 aaaaaagata aatgcagttc aatcgattga cgggcgcttc gaccatagcc ggacaagcat
     6661 tgccccttgg aacagggtgc gatcggcttg aagtcgctcg aggtggtttt ttgtcttgat
     6721 aaacaggtct ttcctgcctt ggtcgccggt gcgaatatgt agaagaattg ccggtttgaa
     6781 atgcgtccgt gtaaacgccg aacggcaagg tcagacaggc tactgcgaat tcagccaatc
     6841 aaacatagtc caaatcccgg tacctctggc ggtaaagcgc cgggatggag cacaaagcaa
     6901 aaaaccgcac taccatttcg atagtgcggt tttcttatgt agtgcgtgaa cgaaaacgcc
     6961 aatctccctg gggaaacctg c
//
LOCUS       DAOSLL010000182         6965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9853_length_6965_cov_4.175253, whole genome shotgun sequence.
ACCESSION   DAOSLL010000182 DAOSLL010000000
VERSION     DAOSLL010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6965
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9853_length_6965_cov_4.175253"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="PLB25_09490"
     CDS             complement(<1..293)
                     /locus_tag="PLB25_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41845.1"
                     /translation="MYPSLQTHPADHSNIQWMPDVQGHRLGTRASTVLAELTSGAVKK
                     GRGQLAALLRRQQLYPVFQPIASLSDGVIFSHEALIRGPLNTDFHAPDALILAA"
     gene            444..1010
                     /locus_tag="PLB25_09495"
     CDS             444..1010
                     /locus_tag="PLB25_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41846.1"
                     /translation="MSPIQPTTPVASPETTAVVEPTTGSTAQAPAATPKTVAAKIVRA
                     RVKKVLLSANPVDQPTVATPVTPVARVATRQPVKKAPAKAVATAVKTASRPAARAVKA
                     AVARPATEKLLKDKKLKMVRDSFTIPKVEYLMLDQLKQRSGILGNAVKKSELIRAGIK
                     ALEALSDANFLAAVKAVPTIKTGRPSNN"
     gene            1137..1985
                     /gene="panC"
                     /locus_tag="PLB25_09500"
     CDS             1137..1985
                     /gene="panC"
                     /locus_tag="PLB25_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HOE41847.1"
                     /translation="MQIIKTIADLREHLSRFKRTSFVPTMGNLHDGHLALVRQARPLG
                     DAVVVSIFVNRLQFLPHEDFDTYPRTWEADCRALELAGCDVLFAPSEKELYPEPQGFT
                     VHPPTELANLLEGHFRPGFFVGVCTVVMKLFACVQPRVSLFGKKDYQQLMVIKRMVQQ
                     FALPIEVIGGQTLRADDGLALSSRNNYLSASERQEAVQLSLALQAMASALRDGEADLS
                     TLEARAMQALERRGWNPDYLTVRQRADLQVPLACRVTSLANNDGLVLLGAARLGSTRL
                     IDNLEV"
     gene            complement(2320..2610)
                     /locus_tag="PLB25_09505"
     CDS             complement(2320..2610)
                     /locus_tag="PLB25_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41848.1"
                     /translation="MLLNVKLVAQIQQGLEVNLANSAGEHPSQHGHVVISARTEAKGK
                     STTLGKVDRRTAPQPRHATLPEPVKAVAQLLANAPGPLTLTQFEAAFKVRGG"
     assembly_gap    2819..2828
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2829..3983)
                     /locus_tag="PLB25_09510"
     CDS             complement(<2829..3983)
                     /locus_tag="PLB25_09510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HOE41849.1"
                     /translation="MNPDAQQLWRAPPWALAVLLATLGMLGPFSIDTYIPAFSGIAAS
                     LHATPVQMQQTLSAYLFGFAFMSLFHGALSDSVGRRPVVLWGLAAFTLASMGCALSQT
                     IGQLVFFRAVQGLTTGAGIVVSRAVVRDMFAPAQAQRVMSQITIYFGVAPAVAPMMGG
                     VLFAHLGWHSVFWFLTLVGLLLWAANYRLLPETLHASQRQSLKLSNLMQGYWKLGRDP
                     RFLLLALASGIPFNGMFLYVLSAPVFLGEHLALVPTQFFWFFVLVISGIMGGAWVSGR
                     MAGRMAPKRQIRNGFLIMIGMAIVNLLANLLSQAHVSWALLPIAVFAFGWAMMVPVVT
                     LLVLDLHPDRRGMASSLQAFIASTANGLVAGVIAPLVMHSTLGLASASMAM"
     gene            complement(4002..4478)
                     /locus_tag="PLB25_09515"
     CDS             complement(4002..4478)
                     /locus_tag="PLB25_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HOE41850.1"
                     /translation="MLYAFLKTLHLLSIIVWVGGMVFAHFFLRPALAEFEPALRLRVM
                     NAVLGRFFAAVLAAASLALLSGGWMIGHVAKRQVQSGVPFNMPLEWIVMAVLGLLMVL
                     IFGHIRFVLYRKLGRAVTGADWPAGALALGKIRLWVLANLVLGVLIILVTLLGSGM"
     gene            4846..5136
                     /locus_tag="PLB25_09520"
     CDS             4846..5136
                     /locus_tag="PLB25_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HOE41851.1"
                     /translation="MKLRPLHDRVIVKRVENETRTASGIVIPDSAAEKPDQGEVLAVG
                     PGKKNDKGELGSMSVKVGDRVLFGKYSGQTVKVDGDELLVMKEDDLFAVVEK"
     gene            5222..6892
                     /gene="groL"
                     /locus_tag="PLB25_09525"
     CDS             5222..6892
                     /gene="groL"
                     /locus_tag="PLB25_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345520.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HOE41852.1"
                     /translation="MAAKDVIFGGEARARMVEGVNILANAVKVTLGPKGRNVVLERSF
                     GAPTVTKDGVSVAKEIELKDKLQNMGAQMVKEVASKTSDIAGDGTTTATVLAQSIIRE
                     GMKYVAAGMNPMDLKRGIDKAVTALIVELKKASKATTTSKEIAQVGSISANADDTIGK
                     IIADAMDKVGKEGVITVEDGKSLESELEVVEGMQFDRGYLSPYFINNPEKQAALLDNP
                     FVLLFDKKISNIRDLLPTLEQVAKSGRPLLIIAEDVEGEALATLVVNTIRGILKVVAV
                     KAPGFGDRRKAMLEDIAILTGGKVIAEEVGLTLEKVTLADLGSAKRIEVGKENTIIID
                     GAGPAADIEARVKQVRVQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGIVAGGGVALLRAKQAAGTIKGDNADQDHGIALVLKAIEAP
                     LREIVYNAGGEASVVVNAVMAGTGNYGFNAANDTYGDMIEMGILDPTKVTRTALQNAA
                     SVASLMLTTECMIAEAPKDDAAGGGMGGMGGGDMGGMGGMGGMGGMGM"
BASE COUNT         1631 a         2059 c         2021 g         1244 t
ORIGIN      
        1 gcagccagga taagggcatc gggggcatga aagtcggtgt tcagcggacc tctgatcaaa
       61 gcttcatgag aaaaaatgac accatcgctc agactggcaa tgggttgaaa gaccggataa
      121 agctgttgcc gacgcagcaa ggcggcaagt tgcccccgcc cttttttgac cgcaccagag
      181 gtcagctcag ccagcactgt actggccctt gtgccaagtc ggtgcccctg aacgtcaggc
      241 atccattgga tgttggaatg gtcggctgga tgggtctgta gggaggggta cattcaaagc
      301 aaaggtccat gtatttgaca ttcaacttta agaaaacccg atgacaggtt tgtgacacgg
      361 actttttttg tcgaaaaagc cacccagacc agactaaacg atataaactg tttacctcgt
      421 ttattcacaa ggattggctc atcatgtcac ccatacaacc aaccaccccg gtagcttcac
      481 cagagacaac tgctgttgtt gaacccacca ccggcagcac ggcgcaagcg cccgcagcca
      541 cccccaagac ggtggctgca aaaattgtca gggcgcgggt caaaaaagtg cttctgtcgg
      601 ccaacccggt tgaccagccc actgttgcga ctccggtaac accagtcgcc agggtagcca
      661 ccaggcagcc ggtcaagaaa gcgccagcaa aggccgtggc aactgcggtc aaaactgcgt
      721 ccagacccgc tgcaagagcc gtcaaggccg cagtcgccag gccagcaacc gagaagctgc
      781 tcaaggacaa aaagctcaaa atggtacgcg acagttttac catcccgaag gttgagtacc
      841 tgatgctcga ccaactcaag cagcgcagcg gcatcctggg caatgctgtc aaaaagagtg
      901 aattgattcg cgctggcatc aaggcgttgg aggcgttgtc cgacgccaac tttctggctg
      961 cggtcaaagc ggttccgaca atcaagaccg ggcgtccgtc caacaactga gaccgcgcca
     1021 gcttgaagcg atgctgcagc cgtgtttggc gggtgcaatg cgagacaata gagcgccgct
     1081 caacggctcc actctacaac tttggcgaac tcattcatcc cgaccaaaca ccagctatgc
     1141 agatcatcaa gaccatcgca gacctgcgcg aacacctgtc gcgtttcaaa cgcacatcct
     1201 ttgtcccgac catgggtaac ctgcatgacg gccatttggc cctggtacgc caggccaggc
     1261 cgctcggtga cgcggtcgtg gtgagcattt ttgtcaatcg cctgcagttc ctgccgcacg
     1321 aggactttga cacctacccg cgcacttggg aggccgattg ccgggcattg gagctcgccg
     1381 gttgtgatgt gttgttcgca cccagcgaga aagagttgta ccccgaacca cagggcttca
     1441 cggttcaccc gcccaccgaa ctggcgaacc tgctcgaagg gcatttcagg cccggttttt
     1501 ttgtcggtgt ctgcaccgtg gtgatgaagc tgtttgcctg cgtacagccc cgggtgtcgc
     1561 tgtttggcaa aaaggactac cagcaattga tggtcattaa acgcatggtc cagcagtttg
     1621 ccctgcccat cgaagtcatt ggtggtcaaa ccctgcgcgc cgatgatggg ttggcactgt
     1681 cgtcgcgcaa caactacctg agcgcgagcg aacgccagga agcggtgcaa ctctcgttgg
     1741 cgcttcaagc catggccagc gcactgcgtg acggcgaggc tgacctctcg actctggagg
     1801 cgcgcgccat gcaggcgctg gagcggcgtg gctggaaccc cgactatctg accgtgcgtc
     1861 agcgcgctga tttgcaagtg ccgctggcat gcagggtcac gtcactggcg aacaacgacg
     1921 ggctggtact gctgggcgca gcgcggctcg gcagcacccg actgattgac aacctggaag
     1981 tttgaccgct caattcggcg tcgccgcgct aagcgcagat tcgctcgaac caccctcccc
     2041 ctttgaatcc ggcctggttg ctccagtgtc ggctacggtc gctggctggc gagttgcacc
     2101 gcctcggtgc ggatttgcag ggctttcaga tagctgttga agcgctgcct gcagcggatt
     2161 gcttcagtca tgctggcctc ggatttgtga cgagttcaag cgtgcaaatg gctgcatcag
     2221 gcttcaggca cgaccccagg ccgcgcggcc tgctgcatca agcggcagcg cccggcccag
     2281 tgcgagcagg attttttggc aacgtcggca gggctttttt cacccaccac gcaccttgaa
     2341 ggcggcttcg aactgcgtca gcgtcagcgg gcctggcgcg ttggccagca gttgcgccac
     2401 cgctttgacc ggctcgggca gcgtggcgtg cctgggttgc ggcgctgtgc ggcggtcaac
     2461 cttgcctaag gtggtgcttt tgcccttggc ctcagtgcgg gcgctgatga caacgtggcc
     2521 gtgctggctc gggtgttctc ctgcactatt ggccaaattg acctctagcc cttgctgtat
     2581 ctgcgccacc agcttcacat tcaatagcaa ttcgatttgc tgctgacgtt atagcgggtc
     2641 ttgaaacgtt gggcgcaacc cgcggttggt gcccgcttga gccgccacgg cgcgttgggc
     2701 agtgagcgca cacaccctat gccgccgggt gctcaatggc ccggccaatc tccggccagc
     2761 gctggtgcag ccagagccag gccaacaagc caacggacag catgctgatg caggccagnn
     2821 nnnnnnnnca tggccatcga ggccgacgcc agccccaggg tggaatgcat caccagcggc
     2881 gcaatcacac cggccaccag cccgttggcg gtcgaggcaa tgaaggcctg caggcttgaa
     2941 gccatgcccc ggcgatccgg gtgcaaatcg agcaccagca gcgtcaccac cggcaccatc
     3001 atggcccagc caaaggcaaa cacggcaatc ggcagcaaag cccaggagac gtgtgcctga
     3061 gacagcagat tggccagcaa attgacgatg gccatgccaa tcatgatcaa aaaaccattg
     3121 cgaatttgtc gctttggtgc catccggccc gccatgcgcc cgctgaccca ggcaccaccc
     3181 atgatgccgc tgatgaccag cacaaaaaac cagaaaaact gcgttggcac cagtgccaga
     3241 tgctcaccca gaaaaacggg ggccgacaac acatacaaga acatgccgtt gaacgggatg
     3301 ccactggcca gcgccagcag aagaaaacgc gggtcgcgtc ccagcttcca gtaaccttgc
     3361 atcaaattgc tgagcttgag ggactgccgc tgcgatgcat gcaaggtttc gggtaacaaa
     3421 cggtagttgg ccgcccacag cagcagcccg accagcgtca aaaaccagaa cacgctgtgc
     3481 cagcccagat gagcaaacaa cacgccaccc atcatcggtg ccaccgcagg tgccacgcca
     3541 aaatagatgg tgatctggct catcacccgt tgcgcctgcg caggcgcaaa catgtcacgc
     3601 accaccgcgc gcgacaccac gatgccagca ccagtggtca ggccttgcac ggcccggaaa
     3661 aacaccagtt ggccgatggt ctgcgacaag gcgcagccca tcgaggccag cgtaaacgcc
     3721 gccagccccc acaacaccac tggccggcgc cccacgctgt cagacagcgc accgtgaaac
     3781 aggctcataa aggcaaagcc aaacaggtag gccgagagcg tttgctgcat ctgcaccggt
     3841 gttgcatgga gcgatgcagc aatgccggaa aacgccggaa tgtaggtatc aatcgagaac
     3901 ggcccaagca tgcccagcgt ggccagcaac accgccagtg cccagggtgg cgcacgccac
     3961 agttgctgtg catcaggatt catggcagtc gtgatgtgcg gtcacattcc cgagccaagc
     4021 aaggtgacaa ggatgatcaa cacgccaagc accagattgg ccaacaccca gagccggatt
     4081 ttccccagcg ccagcgcacc tgcgggccag tcggcaccgg tcaccgcgcg gccaagtttg
     4141 cggtaaagca caaagcggat atggccaaat atcaacacca tgagcaagcc cagcacggcc
     4201 atgacgatcc attcaagggg catgttgaat ggcacgcctg actgaacctg tcgtttcgcc
     4261 acatggccga tcatccagcc gccgctgagc aaagccaggc tggcagcagc cagcacagcg
     4321 gcaaaaaaac gtcccaatac cgcattcatc acacgcaagc gcagcgctgg ctcaaactca
     4381 gcgagggccg gacgcaaaaa gaagtgggca aacaccatgc caccaaccca cacaatgatg
     4441 gacaacaggt gcagggtttt caaaaaggca tagagcatgg cgaacgaccg ggttgatttt
     4501 gcaaggtgtt gagtttgcct gaatatcgcc agccagcgag ttgcgtcaaa cgaaaccggt
     4561 cggcctgggc agcccatccg tgatgggtgc cgtgtgatcg tgtgggcatc cgatgtgatt
     4621 ttcatctgcc cctgcgtccc tgcgggctct gccagacccg gcaaagcatg gggaattgtc
     4681 aaagcagctc ttgaaaagcc atcaaacacc cttatcatta gcactcgcaa gagttgagtg
     4741 ctaacagttc ttcggacaat gctcaccctc ttcaagttaa cgatcactgc cagtaattgg
     4801 cagttttagt ttttaaccat gtgtttcaaa ccattaggag ccactatgaa acttcgtccc
     4861 ctgcatgacc gcgtgattgt caagcgcgta gaaaacgaaa cccgcaccgc ttccggcatc
     4921 gtcattcctg acagcgccgc tgaaaagcct gatcaaggcg aagtgttggc cgttggcccc
     4981 ggcaagaaga acgacaaggg cgaattgggc agtatgagcg tgaaagttgg cgaccgcgtg
     5041 ttgttcggca agtacagcgg ccaaaccgtc aaggtcgatg gcgacgagct gctggtgatg
     5101 aaagaagacg accttttcgc agtcgtcgag aagtaaccat taactattaa atagatagct
     5161 tgtcacgcag catacatgcg ggctggcgac tcatttcact taaatattct ggagtcataa
     5221 catggcagca aaagacgtaa ttttcggcgg cgaagcccgc gcacgcatgg ttgaaggcgt
     5281 gaacattctg gccaacgcgg tcaaagtgac cctgggcccc aagggccgca acgtggtgct
     5341 ggagcgctcg ttcggcgccc ccaccgtgac caaggacggt gtgtccgtgg ccaaggaaat
     5401 cgaactcaag gacaagctgc agaacatggg cgcgcagatg gtcaaggaag tcgcttccaa
     5461 gacttctgac atcgctggcg acggcaccac caccgccacc gtgctggccc agtcgatcat
     5521 ccgcgaaggc atgaagtatg tggcggccgg catgaacccg atggatttga agcgcggtat
     5581 cgacaaggcc gtcacagccc tgatcgtcga gctgaaaaag gcttccaagg ccaccaccac
     5641 cagcaaggaa atcgcccagg ttggctcaat ttcggccaat gcagatgaca ccatcggcaa
     5701 gatcatcgcg gacgcgatgg acaaggttgg caaagaaggc gtgatcaccg tggaagacgg
     5761 caagtcgctc gaatccgaac tcgaagtcgt tgaaggcatg cagtttgacc gtggctacct
     5821 gtcgccctac ttcatcaaca acccggaaaa acaagcagcg ctgctggaca acccgtttgt
     5881 gctgctgttt gacaagaaga tcagcaacat ccgtgacctg ctgccgacgc tggagcaagt
     5941 cgccaagagc ggccgtccgc tgctgatcat tgctgaagat gtcgaaggcg aagcactggc
     6001 aactttggtg gtcaacacca tccgtggcat cctcaaagtg gtggcagtca aggctccagg
     6061 ctttggcgac cgtcgcaagg ccatgctcga agacatcgcc atcctgaccg gtggcaaggt
     6121 gattgctgaa gaagttggtc tgacacttga aaaagtgacg ctggctgacc tcggctctgc
     6181 caagcgcatc gaagtgggca aggaaaacac catcatcatc gacggtgctg gcccggcggc
     6241 tgacatcgaa gcccgcgtga aacaggtgcg cgtgcaaatc gaagaagcca ccagcgacta
     6301 cgaccgtgaa aaactgcaag aacgcgtggc caagctggct ggcggtgttg ccgtgatcaa
     6361 ggttggcgct gccaccgaag tcgaaatgaa ggaaaagaag gcccgcgttg aagacgcact
     6421 gcacgctacc cgcgctgccg tggaagaagg catcgtagct ggtggcggcg tggctctgct
     6481 gcgcgccaag caggccgctg gcaccatcaa gggtgacaac gctgaccagg accacggtat
     6541 tgccctggtg cttaaagcca tcgaagcgcc gctgcgcgaa attgtttaca acgccggcgg
     6601 cgaagcctcg gtggtggtga acgcggtgat ggccggcacg ggcaactatg gcttcaatgc
     6661 cgccaacgac acctacggcg acatgatcga aatgggcatt ctggacccga ccaaggtaac
     6721 ccgcactgcg ctgcaaaacg cagcttcagt agcctcgctg atgttgacca ccgaatgcat
     6781 gattgccgaa gcgccaaagg atgacgcggc tggcggcggc atgggcggca tgggcggcgg
     6841 cgacatgggt ggcatgggcg gcatgggcgg catgggtggc atgggcatgt aatttgtccg
     6901 gttcgccgat gcgccggatg gcgctcaact tgacaaaaac cccgcaaagc gtaagccttg
     6961 cgggg
//
LOCUS       DAOSLL010000183         6954 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9878_length_6954_cov_4.756486, whole genome shotgun sequence.
ACCESSION   DAOSLL010000183 DAOSLL010000000
VERSION     DAOSLL010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6954
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9878_length_6954_cov_4.756486"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1560)
                     /gene="phaC"
                     /locus_tag="PLB25_09530"
     CDS             complement(<1..1560)
                     /gene="phaC"
                     /locus_tag="PLB25_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HOE41853.1"
                     /translation="MTDGWKNAFASFQNMDLGAANANWKATDVKPPKISFSPKKLQQL
                     QRDYLKEVTELWNNPPTAPKFDDRRFADPAWSANPATAFSVAAYLLNSKVLMQMVDTV
                     KADDMTLARIRFTVEQMIAAAAPSNFMALNAEAHQKAIASNGESIAKGVQNLVRDIGQ
                     GRLSMTDESQFVIGKNVATTEGAVVFENDIFQLIEYKPLTATVHERPLLLIPACINKF
                     YILDLQPDNSVIRYAVSQGYRTFVMSWRNPDASMGKKTWHDYIEDGAIKAIMLARQIS
                     GADAINALGFCVGGTILSNALAVLAARGEKVVCSATFLTSLIDFTDTGILSIFIGEQV
                     VKYREQELAGGGLMKGADMASIFSFLRPNELVWNYVVGNYLKGETPPPFDLLYWNADS
                     TNLPGPFYTWYLRNTYLENNLIKPGVTTVCGETIDFGQVDMPAYIYCSREDHIVPISG
                     AYATTQHLPGNKRFVMGASGHIAGVINPPSKNKRCYWIREDGQLPKSVEQWQEGATEH
                     PGSWWPDWTNWL"
     gene            1720..2040
                     /locus_tag="PLB25_09535"
     CDS             1720..2040
                     /locus_tag="PLB25_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41854.1"
                     /translation="MYIVPIAWLYVTLMMALAEATSSQGSVLGAVITFVLYGVLPISI
                     VVYIMATPARRRAIKARELADLQAAQAAQANVSAPPCSASGNTPDAGGKTPADAVAAM
                     RKEA"
     gene            complement(1969..2751)
                     /gene="pgeF"
                     /locus_tag="PLB25_09540"
     CDS             complement(1969..2751)
                     /gene="pgeF"
                     /locus_tag="PLB25_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HOE41855.1"
                     /translation="MMAGWLVPDWPAPAGIRAVCTTRVGGVSAVPYDSLNLGDHVGDQ
                     ADLVANNRAIFQQAIGVQPIFLNQVHGTKVLQLDAATPHACSADACLTNQLGIACTIM
                     VADCLPVLLTTTRGDWVAAAHAGWRGLAGLDGIGVLETTCKSFGALALTEYALAATDI
                     IAWLGPCIGPQAFEVGDEVRATFVAHDALALPLFEARQPGKWLADLAGLARQRLARLG
                     ITRVYGNDSSPAWCTASQPSRFFSHRRDRISGRFAASIWRVA"
     gene            complement(2748..4601)
                     /gene="ycaO"
                     /locus_tag="PLB25_09545"
     CDS             complement(2748..4601)
                     /gene="ycaO"
                     /locus_tag="PLB25_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HOE41856.1"
                     /translation="MVDNLVGERFARRSTGRSPSPGAVGAGLTWAMLRCYAAPMHNHT
                     FIPGKDAALESTISTLQGKLQQLGFHIEERSWLNPVDGIWSVHIRDRDCPLLFSNGKG
                     ATRLACQASALGEFMERLSTNYFWTHFYLGLDRVNREYVHYPQERWFEVPEDGTWPAE
                     LLTPELQQLYNPQGTVDASSLVDFNSGFDERGICTIPYVRERDGQAVWFPVNIIGNLY
                     VSNGMSAGNTQMEARTQALSEIFERAIKARIISEGICLPDVPEAVIARYPRIARGIAA
                     LREAGFGILVKDASLAGQYPVMNVTLLNPLDQGCFASFGAHPRFEIALERALTELLQG
                     RALDALSDFPSPGFDLEETASSTNIEIHFVDSSGVIHWKFLGNTPDFEFVDWNFSSST
                     AADYAWCVQRLQREGYDIYVADFNHLGVYACRILVPGLSEIYPIDDLEFENNSIANPI
                     REAILNLTDLDDDECADLLETLNESNLADHRPVPGLIGLAAEPDSFWSNLRLGELKTL
                     LALAIGDEAATLEGCEWILNFDQIDAQRKRVYACIQTLIHLADMGDSGPYLDALSLLY
                     GAGPLAQAHALIMQEDRFMGLAAPGLALVGCEMHHQLLLAFDKVRAAHVMA"
     gene            4873..6765
                     /gene="phaC"
                     /locus_tag="PLB25_09550"
     CDS             4873..6765
                     /gene="phaC"
                     /locus_tag="PLB25_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HOE41857.1"
                     /translation="MSVPESKKPPKVERRATGPIASIAGAILSAHEDRVAPDPKELAK
                     AYADVAKRTSAMIQNHIKRQMKKGVSMPTDDLGIAKAFMDMTQKMLADPQKMVEAQMQ
                     LMQDYFALWQHSMMRAMGMDGDPVTAPKKGDNRFKNKDWDELFMFDFIKQSYLITANH
                     VHGAVNSVEGLDENSQKKVDFFTRQFIEALAPTNFALTNPDVLRETVKTSGQNLLGGF
                     KNLLRDIEAGDGELRVKMADTSAFEMGRNVATAKGKVIFQNEMIQLIHFTPTTTEQYK
                     RPLLIVPPWINKYYILDLRESNSWIKWTTDQGHNVFVISWVNPDAKLAQKGFDAYLAE
                     GALTAIDVVCDQTGEKEINTIGYCLGGTLLASTMGYMAAKKDKRVASATFFVALLDFT
                     IPGELGVFIDEKQISFLEKKMSERGFMEGTEMAGTFNMLRSNDLIWTFVINNYLLGKE
                     PFPFDLLFWNSDSTRMPYAMHSFYLRNMYLNNRLREPGGITLLDEPIDLSKVTTPCYF
                     ISTVEDHIAPWKGTYLGATALGGPVRFVLGGSGHIAGIVNPPSANKYGYWTNDAQPTS
                     MPADDWLAGAKQTPGSWWNDWQAWVTGLNGAKVPARDPMVGKYKPIGDAPGSFASVRL
                     DAPAKK"
BASE COUNT         1561 a         2020 c         1946 g         1427 t
ORIGIN      
        1 cagccagttt gtccaatcgg gccaccaact gccagggtgt tcagtggcac cttcttgcca
       61 ctgttccacc gactttggca attgcccgtc ttcccgaatc cagtaacagc gcttgttctt
      121 ggaaggcggg ttgatcacgc cggcaatatg gccagaagct cccatgacaa agcgcttgtt
      181 gcccggcaag tgctgggtcg tggcataggc accgctgatc ggcacgatgt ggtcttcgcg
      241 cgagcaataa atgtaggcag gcatgtccac ttggccgaaa tcgattgttt cgccgcacac
      301 ggtagtgaca ccgggtttga tgaggttgtt ttcaaggtaa gtgttgcgca aataccaggt
      361 gtaaaaaggc cctggcaaat tggtgctgtc ggcgttccag tacagcaggt caaacggcgg
      421 cggggtttca cccttgaggt agttgcccac tacatagttc cagaccagct cattggggcg
      481 caggaagctg aagatgctgg ccatgtcggc ccccttcatc aatccgccac cggccagttc
      541 ctgctcgcgg tatttcacaa cctgttcgcc aatgaagatg gacagaatgc cggtgtcggt
      601 gaaatcgatc aacgaggtca aaaaggtggc gctgcagacc accttttcgc cgcgtgccgc
      661 caacacggcc agcgcgttgc tcaaaatcgt gccaccgaca cagaaaccca gcgcattgat
      721 ggcatcggca cccgaaatct gccgggccag catgatcgcc ttgatggccc cgtcttcgat
      781 gtagtcgtgc caggtcttct tgcccatcga cgcatccggg ttgcgccagc tcatgacaaa
      841 ggtgcgatac ccttgcgaga cggcgtaacg gatcaccgag ttgtcaggct gcagatcaag
      901 aatgtagaac ttgttgatac acgctggaat cagcagcagc ggccgctcat gcactgtggc
      961 agtcaggggc ttgtactcga tcaactgaaa gatgtcgttc tcaaaaacaa cagcaccttc
     1021 ggtggtggcg acatttttgc caatgacaaa ctggctctca tcagtcatcg acaagcggcc
     1081 ttgcccgatg tcgcgcacca ggttttgcac acctttggca atgctctcgc cattgctggc
     1141 gatggccttt tgatgcgcct cggcgttgag cgccatgaaa ttgctggggg ccgctgctgc
     1201 aatcatctgc tcgacggtga aacgaatgcg cgccagcgtc atgtcgtcgg ccttgaccgt
     1261 atcgaccatt tgcatcaaaa ctttggaatt aagcaagtag gcagccaccg aaaaagcagt
     1321 agccggattg gccgaccagg ccgggtcggc aaaacgacga tcatcaaact tgggtgcggt
     1381 cggagggtta ttccaaagct cggtgacctc tttcaggtag tcccgctgca actgctgcag
     1441 cttcttgggt gagaaagaga tttttggcgg tttgacatcg gtggccttcc agttggcatt
     1501 ggccgcaccc aaatccatgt tctgaaatga cgcgaaggca tttttccaac cgtcggtcat
     1561 gctttgctga aattgcttcg cggaacgctg ccaaatgtcg cctgtgttgt ccaccatttt
     1621 ttgtccttta gtgcgtgaaa aaaaacggct agtatcttct ctagcgaata acaatgcgcg
     1681 cgctgcctta aattattatt tcaacggttt caactcacca tgtacatcgt gcccatcgcc
     1741 tggctttatg tcactttgat gatggcgctc gccgaggcga ccagcagcca gggttcagtg
     1801 ctgggggcgg tgattacttt tgtgctgtat ggcgtgctgc caatcagcat tgtggtttac
     1861 atcatggcca cgcctgcgcg caggcgggcc atcaaggcgc gcgagcttgc tgacctgcag
     1921 gccgcgcagg ccgcgcaggc caacgtctct gcgccccctt gcagtgcctc aggcaacacg
     1981 ccagatgctg gcggcaaaac gcccgctgat gcggtcgcgg cgatgcgaaa agaagcgtga
     2041 cggctggctg gcggtgcacc atgccgggct gctgtcgttg ccgtagaccc tggtgatgcc
     2101 caggcgcgcc agccgctgtc gcgccagccc ggccagatcg gccagccact tgccaggctg
     2161 gcgggcttca aacaggggca gcgccagcgc atcatgggca acgaaagtgg ctcgcacctc
     2221 gtctcccacc tcaaaggctt gcggaccgat gcaaggcccc agccatgcta ttatatcagt
     2281 agctgccagc gcatattctg taagggctag agcaccaaaa ctcttgcaag ttgtttccag
     2341 aacaccgatg ccgtccaggc ctgccaggcc acgccagcca gcgtgtgccg ccgccaccca
     2401 atcgccccgt gttgtggtca gcagcaccgg caggcagtct gccaccatga tggtgcaggc
     2461 gatgcccaac tggtttgtca agcaggcatc ggcgctgcat gcgtgggggg tggcggcatc
     2521 caattgcagg actttggtgc catgcacctg attcaaaaaa atcggctgca ccccgattgc
     2581 ctgttgaaaa atagccctgt tatttgccac caggtcagcc tggtcaccga catgatcacc
     2641 caggttcagg ctgtcatacg gcactgccga tacaccgccg acacgggtgg tgcagaccgc
     2701 gcggatgcca gcaggcgcgg gccaatcagg taccagccaa ccagccatca cgccatcaca
     2761 tgggccgcac gcaccttgtc aaaggccagc agcaactgat ggtgcatttc acaaccaacc
     2821 agtgccagcc caggtgccgc caggcccatg aagcggtctt cctgcatgat caaggcatgc
     2881 gcttgcgcca gcggcccggc accgtagagc agcgacaagg catccagata tggcccgcta
     2941 tcgcccatgt cagcgaggtg aatgagagtc tggatgcagg cgtacacgcg tttgcgctgg
     3001 gcatcgatct ggtcaaagtt cagaatccac tcacagcctt ccagcgtggc ggcttcgtcg
     3061 ccaattgcca gcgccagcag ggttttgagc tcaccgaggc gcaagttgct ccagaacgaa
     3121 tccggctcgg cagccagtcc gatcaagcca ggcacgggtc gatgatcggc caggttggac
     3181 tcgttgaggg tttcaagcag atcggcgcat tcgtcgtcgt caaggtcagt cagattcagg
     3241 atggcttcac gaatcggatt ggcaatgctg ttgttctcga actccaggtc gtcaatcggg
     3301 taaatctcgg acaaaccagg taccagaatg cggcaggcat agacaccgag gtggttaaag
     3361 tcggccacgt aaatgtcgta gccctcgcgc tgcagtcgct gcacgcacca ggcgtagtcg
     3421 gcggccgtgc tgctgctgaa gttccagtcg acaaactcaa agtcaggggt gttgcccaga
     3481 aatttccagt ggatcacgcc gctggaatct acaaaatgga tttcgatatt ggttgaactt
     3541 gcggtttctt ccagatcaaa gccaggcgac ggaaagtctg acagtgcgtc aagcgcccta
     3601 ccctgcagca gttcggtcaa tgcacgctca agagcgatct caaatcgcgg atgcgcgcca
     3661 aagctggcga aacaaccctg atccagaggg ttaagcaagg tcacattcat caccggatat
     3721 tggcctgcta gcgacgcatc tttcacaagg atgccaaacc cggcttcgcg cagcgccgca
     3781 atgccgcgcg cgatgcgggg atagcgcgca atcaccgcct cgggcacatc gggcagacaa
     3841 atcccttcgc tgatgatgcg ggccttgatg gcacgctcaa aaatctccga aagcgcttgg
     3901 gtgcgggcct ccatttgggt attacctgcg gacatgccat tgctgacata caggttgcca
     3961 atgatattga ccggaaacca cacggcctga ccgtcacgtt cacgcacata gggaatggtg
     4021 caaatgccgc gctcgtcaaa gccggaattg aagtccacca ggctgctggc atccaccgtg
     4081 ccttgcgggt tgtagagttg ctgcaactct ggggtcaaca gttcggcggg ccaggtgcca
     4141 tcctcgggaa cctcaaacca gcgttcctgc gggtaatgga catattcccg attaaccctg
     4201 tcaagcccga gataaaaatg cgtccagaaa taattggtgg acaggcgctc catgaactcg
     4261 cccaaagcgc ttgcctggca ggccagccgg gtcgccccct tgccgttgga aaacagcagc
     4321 gggcagtcac ggtcgcggat atgcaccgac cagatgccat cgaccggatt gagccagcta
     4381 cgttcctcaa tgtgaaagcc gagttgctgg agcttgccct gcagcgtgct gatggtggat
     4441 tcaagggcgg catctttgcc aggaatgaag gtgtggttgt gcatgggtgc agcataacag
     4501 cgcagcatcg cccaggtcag gccagcgccg acagcgccgg gcgaaggcga ccggccggtg
     4561 ctgcgccggg caaatcgctc gccaactaaa ttgtcaacca attcactctg gattggcaga
     4621 ctttgtcttg cccgaattta aattacattt cagcttgtcc gacctgagaa agcccggcta
     4681 gcgaccagaa aacggtcaga accaaggata aaataaaggt tcgtcatcgg acccactcat
     4741 ggagacactg cctgatcgat ctgattgcct gcccaagcct gaccggctcg gccaccgggt
     4801 tggcgctgaa atgattcatc gcctctaaac gaaccgtatt tccacttttt caattaacaa
     4861 aatggagtca ctatgtcagt tccagaatcc aagaaacctc ccaaagttga gagacgagcg
     4921 accggtccga ttgcttcgat agccggggcc attctgtcgg cccatgaaga ccgggtcgca
     4981 cccgatccca aggaacttgc caaggcctat gccgatgttg ccaagcgcac ctcggcaatg
     5041 atccagaacc acatcaagcg tcagatgaag aaaggcgtgt caatgcccac cgacgatctg
     5101 ggtatcgcca aggcattcat ggacatgacg caaaagatgc tggccgaccc gcagaaaatg
     5161 gtcgaagcgc aaatgcagtt gatgcaggat tattttgcgc tgtggcagca ttcaatgatg
     5221 cgcgccatgg gcatggacgg tgatccggtt accgcaccca agaagggcga caaccgcttc
     5281 aaaaacaagg actgggacga actgttcatg tttgacttca tcaagcaaag ctacctgatc
     5341 acggccaacc acgtgcatgg tgccgtcaac agtgtcgaag gcctggacga aaacagccag
     5401 aaaaaggttg atttcttcac ccgccagttc attgaagcgc tggcaccgac caacttcgcc
     5461 ttgaccaacc ccgatgtgct gcgcgaaacc gtcaagacca gcggccagaa cctgctcggt
     5521 ggcttcaaga acctgctgcg cgacattgag gcaggtgacg gtgaactgcg cgtcaagatg
     5581 gctgacactt ccgccttcga gatgggtcgc aacgtcgcca ccgccaaggg caaggtgatt
     5641 ttccagaacg aaatgatcca gttgatccac tttacgccga ccaccaccga gcaatacaag
     5701 cgcccgctgc tgatcgtgcc gccgtggatc aacaagtact atattcttga cctgcgcgaa
     5761 agcaattcct ggatcaaatg gaccaccgat caggggcaca acgtgtttgt gatctcctgg
     5821 gtcaatccgg atgccaagct ggcacaaaag ggctttgatg cctacctggc cgaaggcgca
     5881 ctgaccgcca tcgacgtggt ttgcgaccag accggcgaga aagaaatcaa caccatcggc
     5941 tattgcctgg gcggcaccct gctggcctcg acaatgggct acatggccgc caagaaagac
     6001 aagcgcgtgg cttctgccac cttctttgtg gcgctgctgg acttcaccat tccaggcgaa
     6061 ctcggtgtct tcattgatga gaaacagatc tcgttccttg agaagaagat gtcggagcgc
     6121 ggcttcatgg aaggcacgga aatggccggc accttcaaca tgttgcgatc caacgacctg
     6181 atctggacct ttgtcattaa caactacctg ctgggcaagg aaccgttccc gtttgacctg
     6241 ctgttctgga actctgattc cacccgcatg ccatacgcga tgcacagctt ctaccttcgc
     6301 aacatgtacc tcaacaaccg tctgcgcgaa cccggcggta tcaccctgct tgacgagccg
     6361 attgatctgt caaaggtcac gaccccttgc tacttcattt ccaccgtcga ggaccatatt
     6421 gcgccgtgga agggcaccta tctgggtgcc accgccctgg gtggtccggt gcgcttcgtg
     6481 ctgggcggct ccggtcacat cgctggcatc gtcaatccgc cgtctgccaa caagtatggc
     6541 tactggacca acgatgcgca gccgacatcg atgccggccg acgactggct cgccggtgcc
     6601 aaacagactc ccggttcctg gtggaatgac tggcaggctt gggtcaccgg gctcaacggt
     6661 gccaaagtgc cagcgcgtga cccgatggtc ggcaagtaca aaccgattgg cgacgcgcct
     6721 ggttcctttg cttcggtgcg gcttgacgcg ccagccaaga aatagtcggc tagtccggta
     6781 attaccgcaa cgggtgtgaa gctgagaagc ttcacacccg ttttatattg ccgacacggt
     6841 ggtatggctg cgatctgcgt cttgtttgat acggcctgca tcaaacccga aagacaaacc
     6901 agtgtaagcg ggtgggcaag tcaccttgcg ggacgctgag attttcaaga ccat
//
LOCUS       DAOSLL010000184         6949 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9885_length_6949_cov_4.521033, whole genome shotgun sequence.
ACCESSION   DAOSLL010000184 DAOSLL010000000
VERSION     DAOSLL010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6949
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9885_length_6949_cov_4.521033"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /gene="pilM"
                     /locus_tag="PLB25_09555"
     CDS             complement(<1..380)
                     /gene="pilM"
                     /locus_tag="PLB25_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOE41858.1"
                     /translation="MLKATKLSKVNQVIVAQKSLRDNALADSIEVIYQEGCRFFICKG
                     MQLFSLGSLFSRQPAAMLGLDISSSSVKLVELGRDKAGCYVLENYAILPLERGWITDG
                     NIVKFDEVADAVRRLIKKSGTKTR"
     gene            587..2896
                     /locus_tag="PLB25_09560"
     CDS             587..2896
                     /locus_tag="PLB25_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HOE41859.1"
                     /translation="MKSLLWTGTLLVACFLSVLLLVAIAMAMAFPNLPDISDLSDYRP
                     KLPLRVFSAEGTLIGEFGEERRHLTPIKDIPRVMKDAVLAIEDARFYEHGGVDYKGIA
                     RAALANLGRIKSQGASTITMQVARNVYLSSEKTYTRKIYEVLLTFKLEHMLTKDQILE
                     VYMNQIFLGNRAYGFAAAAEAYFGKPLKDITVAEAAMLAGLPKAPSAYNPIINPKRAR
                     SRQLYIIERMLENGFITPEQAEAANKEELKVRISSDHSSVHAEFVAETVRQLIFAQYG
                     ADTYTRGLNVYTTLQAADQLLAYQALRRGIMNYERRQVYRGPEKFVSLPTDMAQAEDI
                     IDDTLLEHPDNGDVMSAVVLEASPKLVKAIRQSGEIIEISGDGLKPAQSGLSEKAAAH
                     IRIRRGAVIRVMQTPKSNWEITQLPEVEGAFVALDPRNGAIKALVGGFDFEKSKFNHV
                     TQAWRQPGSSFKPFIYSAALEHGVTPATIINDSPLFFNAGVTGGQPWEPKNYDGKFEG
                     PMSMRRGLAKSKNMISIRILQTVGAQNAQQWVTRFGFDADKHPAYLTMALGAGSVTPM
                     QMATAYSVFANGGYRINPWLITRVTDQQGKLLRQTQPAVLNDSVRAIDARNAFIMNSL
                     LQEVTRSGTAASAQGTLKRPDLYGKTGTTNDAMDAWFAGFQPSLAAITWIGYDTPRKL
                     GDRETGGGLSLPIWIEFMAQALKNVPVMEQPVPEGIIHSGGEWYFAEYAPGAGVTSLG
                     LQDGAEATPQVMPVPAADEKKKILDLFKN"
     gene            complement(3114..3443)
                     /gene="cyaY"
                     /locus_tag="PLB25_09565"
     CDS             complement(3114..3443)
                     /gene="cyaY"
                     /locus_tag="PLB25_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HOE41860.1"
                     /translation="MTDSEFMDVAERALKAVEASCDRINDNTEADIDNQRTGGMITLA
                     FANRSQIVINLQKPLHEIWLAARCGGFHYRFDGAHWIDTKGQGEFFDSLSRFASDQSG
                     VALVFSA"
     gene            3465..3656
                     /locus_tag="PLB25_09570"
     CDS             3465..3656
                     /locus_tag="PLB25_09570"
                     /inference="COORDINATES: protein motif:HMM:NF025012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HOE41861.1"
                     /translation="MFQILVRALCLGCCVAVLSACGQQGPLYLPTQAAAASRASLPQS
                     LLPSKTPASQPAKPISSPP"
     gene            complement(3698..4336)
                     /locus_tag="PLB25_09575"
     CDS             complement(3698..4336)
                     /locus_tag="PLB25_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HOE41862.1"
                     /translation="MHAMAFPGHLPIGRLLLRPWAKPLVFALALLPCVTLLWAVGFDQ
                     LGANPAEALIRATGDWTLRMLCLVLAVTPLRVIFAVPTLARFRRMLGLFVYFYACLHL
                     LSYAWFDMGFEMTDVLRDIAKRPFILVGFVGFVLLSVMALTSHNLVIKRVGGKIWKRL
                     HQSVYVVAGLALLHFFWMRAGKNNFAEVIVYAVVLAALLAWRVVHFARKKAL"
     gene            complement(4336..5334)
                     /gene="msrP"
                     /locus_tag="PLB25_09580"
     CDS             complement(4336..5334)
                     /gene="msrP"
                     /locus_tag="PLB25_09580"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HOE41863.1"
                     /translation="MQISTRRDGFNHPESSSITPLAIYQSRRDLIRLMATGVAGAALG
                     SLVARQAFAQSVALPGKNVALPGSRTRVAGGVTMDKVTDYKDASSYNNFYEFGTDKAD
                     PAKNAHTLKISPWAVEIEGMVKKPARYTLEDLIKLSAQEERIYRLRCVEGWSMVLPWV
                     GYSLSELIKKVEPLGSAKYVEFVTLADPRTMPGVGSRVLDWPYVEALRMDEAMHPLTL
                     LSFGMYGEVLPKQSGAPVRLVVPWKYGFKSGKSLVKIRFTEKQPTTAWNRVAPSEYGF
                     FANVNPDVDHPRWSQATERRIGEDGLFAKKRKTLIFNGYEPQVGQLYAGMDLKKFF"
     gene            complement(5543..6169)
                     /locus_tag="PLB25_09585"
     CDS             complement(5543..6169)
                     /locus_tag="PLB25_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOE41864.1"
                     /translation="MKLIAHFMIAATMAATVSVCSAEGAAAPAAKHDLAKGEASFAVC
                     AACHGPDGNSVIPVNPKLAQQHPEYLVKQLQEFKVGKRANAIMTGMSAPLSDEDMKNI
                     AYWVSSKKSTPGSAANKESLILGERIYRGGIADRKVPACAGCHSPTGAGIPAQFPRLS
                     GQHADYAAAQLTAFRDGVRQNSLQMTQVAARLNDREIKAVADYVAGLR"
     gene            6319..>6949
                     /gene="yihA"
                     /locus_tag="PLB25_09590"
     CDS             6319..>6949
                     /gene="yihA"
                     /locus_tag="PLB25_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HOE41865.1"
                     /translation="MALGWLHTAKFLTTAPQLHFLPPLSVPEIAFVGRSNAGKSTCIN
                     TLTQQKQLAFASKKPGRTQHINLFSLGKQGITDAVLADLPGYGYAAVPRQDKIRWQQV
                     MANYLVTRENLKAIVLMCDPRHGLTELDEILLDVVRPRVEEGLKFLVVLTKADKLTRS
                     EQAKALSIMKLQAGGGEVRLFSATKRQGLDEVATLLWHWAHPAPLTDTEV"
BASE COUNT         1642 a         2029 c         1844 g         1434 t
ORIGIN      
        1 tttctcgttt tagtgccgct ttttttgatc agacgtcgca ctgcatcggc cacttcgtcg
       61 aacttcacaa tatttccatc ggtgatccag ccacgctcta gcggcaaaat cgcgtagttt
      121 tccaagacat aacatccggc cttgtcgcgc cccaattcaa ccagcttgac gcttgatgaa
      181 ctgatatcca gacccagcat ggctgcgggt tggcggctga ataatgaccc caaagaaaac
      241 aactgcatcc ccttgcaaat gaaaaaacgg catccctctt gataaatcac ttcaatgcta
      301 tcagcaagag cgttgtcacg caaagacttt tgcgcaacta tcacttgatt aaccttgcta
      361 agtttagtcg ctttaagcat ccgcaggctc ttgctgcatc aagtggggtt ccgcaccaag
      421 gcatttgacc aacagtggga tactaaccgg ttcatgcgac acgctctcat ttttacaatg
      481 aatactgtct aacacctgtt tttttatgcc cgcaaaagcc ccacaagtct cagataaccc
      541 tgaaataaca tcgcgccacc caacaagtgc cctgagccgg tgggtcatga aatcgttgct
      601 atggacaggc accctgctgg ttgcctgctt tttgtcggtg ctgctgctgg tcgcgattgc
      661 catggccatg gcttttccaa acctgcccga catttcagac ctgtcggatt accggcccaa
      721 gctgccgctg cgcgtttttt cggctgaagg caccctgatt ggtgaatttg gcgaagagcg
      781 acgccacctg acgcccatca aggatattcc aagggtgatg aaagatgcag tgctggccat
      841 tgaggatgcg cgtttctacg agcatggtgg ggtcgattac aagggcatcg ccagagcggc
      901 actggccaac ctggggcgca tcaaaagtca aggagcctcc accatcacca tgcaggtcgc
      961 acgcaatgtc tatttatcgt cggaaaaaac ctatacccgg aaaatatatg aagtcttgct
     1021 gacgttcaag ctcgagcaca tgctgaccaa agaccagatt cttgaggtct atatgaacca
     1081 gatatttttg ggcaaccggg cctacggttt cgctgcggca gccgaagcct attttggcaa
     1141 gccactcaag gacatcacag tcgccgaagc cgccatgctg gcgggcttgc ccaaggctcc
     1201 atctgcctac aacccgatca tcaaccccaa gcgcgccagg tcgcgtcagc tctacatcat
     1261 tgaacgtatg ctggaaaatg gctttatcac gcctgagcag gcagaagcgg ctaacaagga
     1321 agaactgaaa gtcaggatca gttcagacca cagcagcgtg catgcagaat tcgtcgcgga
     1381 gaccgttcgc caattgatat tcgcgcaata cggtgccgac acctacaccc gtggactcaa
     1441 cgtgtacacc acgctgcaag cggctgacca gttgctggct taccaggcat tgcgtcgggg
     1501 catcatgaac tatgaacgcc gtcaagttta ccgggggcca gaaaaattcg ttagtctgcc
     1561 aacggatatg gcccaagccg aagacatcat tgacgacacc ctgctggagc atccggacaa
     1621 cggtgatgtc atgtctgcag tggtgctgga ggctagccca aaactggtca aggcgatacg
     1681 ccaaagcggg gaaatcatcg agatcagcgg cgacggcctt aagccggccc aatcgggcct
     1741 gtcggaaaag gcggccgccc acatccggat tcgccgaggt gcagtcatca gggtgatgca
     1801 aacgccaaaa tccaactggg aaatcaccca acttccagaa gtcgagggcg cgtttgttgc
     1861 gctagaccca cgaaatgggg cgataaaggc cctggtgggt ggttttgact ttgaaaaaag
     1921 caagttcaac catgtgacgc aggcatggcg gcagccaggc tccagtttca aaccgtttat
     1981 ctattcggca gcgctggagc atggcgtgac cccggccacc atcatcaacg attcgccgct
     2041 gtttttcaac gcgggggtga ccggcgggca gccctgggag ccgaaaaact acgatggcaa
     2101 gtttgaaggc ccgatgagca tgcgtcgcgg tctggccaag tccaagaaca tgatttcgat
     2161 ccggatcttg cagaccgtag gcgctcaaaa cgcacaacaa tgggtgaccc gatttggttt
     2221 cgatgccgac aagcacccgg cctacctgac catggccctg ggtgccgggt cggtgacacc
     2281 gatgcaaatg gcaaccgcat actcggtgtt tgccaatggc ggctaccgca tcaacccatg
     2341 gttgatcacc cgggttaccg accagcaagg caagttgttg aggcaaaccc aaccagccgt
     2401 gctaaacgat tcggtgcgag cgattgatgc acgcaatgcc tttatcatga acagcttgtt
     2461 gcaggaggtc acccgttccg gcaccgctgc cagcgcacag ggcaccctga agcggccaga
     2521 tctgtatggc aagacaggta ccaccaacga tgccatggat gcctggtttg ccgggtttca
     2581 acccagtctg gctgccatca cctggatcgg ctacgacaca cccagaaaac tcggtgacag
     2641 ggaaaccgga ggtggcttga gcctgccaat ctggattgag ttcatggcgc aagcgctgaa
     2701 aaacgtgccg gtcatggagc aaccggttcc cgaaggcatc atccattccg gaggtgaatg
     2761 gtattttgcc gagtatgcgc caggtgctgg cgttacctct ctggggcttc aggatggtgc
     2821 cgaagcgaca cctcaggtca tgccggtacc ggccgccgac gagaaaaaga aaattctgga
     2881 tttgttcaag aactagtaga tcgtcgaact cgtggatcga accgccataa atagactgcg
     2941 gcaaagccag tttgcaccga attcgcaggc ccattgccct gcgctgggtc aatgaagccg
     3001 ttgcaaaaag gaccgcactg cggttggtgt gcagccgatg ttcgacatct ccgacaggct
     3061 cgcagcgcat ggcagacggg ttctacacgc gctgccaaaa aaatcagcca tcgtcatgca
     3121 gaaaaaacca gcgcgacacc ggactggtca ctggcgaagc gtgacaggga atcaaaaaac
     3181 tcaccctgac ccttggtatc aatccaatgt gcgccgtcaa atcggtagtg aaaaccacca
     3241 cagcgagcag ccagccagat ttcgtgcagt ggcttttgca ggttgatgac gatctggctg
     3301 cggttggcaa acgccagagt gatcatgccg ccggttcgct ggttgtcgat gtccgcctcg
     3361 gtgttatcgt taatacggtc acaacttgcc tccaccgctt tgagtgcccg ttcggccacg
     3421 tccatgaatt ctgagtcagt cattacaatt tcaccatgtt gttcatgttc caaattctag
     3481 ttcgcgcact ctgccttggc tgctgtgtgg cggtgttgtc agcctgcgga caacaaggcc
     3541 cgctgtactt gccgacccaa gccgcagcag ccagccgggc cagcctgcca caatcgttgc
     3601 taccatccaa aacacccgcc agccagccag ccaagccaat cagcagtccg ccatgacgct
     3661 atattaaata tagctatcgg cgcccgtcac ataagcgcta gagggctttt ttccttgcaa
     3721 agtggacgac ccgccaggca agcaaagcag ccagtacaac agcgtagaca atgacttcgg
     3781 caaagttgtt tttgcccgcg cgcatccaaa agaaatgcag cagggccaag cccgcaacca
     3841 cgtaaaccga ctgatgtaac cgtttccaga tttttccacc cacacgcttg atgaccaagt
     3901 tgtgcgaggt gagcgccatc accgaaagca gcacaaaacc gacaaaccca accagaatga
     3961 acggccgctt ggcaatatcc cgcaaaacat cggtcatctc aaaccccatg tcaaaccagg
     4021 cataactcag caggtgcagg caagcataga aatagacaaa caggcccagc atgcgccgaa
     4081 acctggccag ggtcggcacc gcaaagatga cccgcagcgg cgttaccgcc agcaccaggc
     4141 acaacatgcg cagggtccag tcgcctgtgg cgcgaatcag ggcttctgcc gggttggccc
     4201 ccaactgatc gaagccgacg gcccagagca acgtcacaca aggcagcagc gccagcgcga
     4261 aaaccagcgg cttggcccag ggacgcagca atagcctgcc gatcggcaag tggccaggaa
     4321 aagccatggc atgcatcaga aaaacttctt caggtccatg cctgcataca actgaccgac
     4381 ctgtggctca tagccattga aaatcagggt cttgcgcttc ttggcaaaca gaccgtcttc
     4441 accaatgcgc cgctcagtcg cctggctcca gcgcgggtga tcaacatcgg ggtttacgtt
     4501 ggcaaaaaag ccgtattcac ttggtgcgac ccggttccag gcggtcgtcg gctgtttctc
     4561 ggtgaatcgg atcttgacca ggcttttgcc actcttgaaa ccatatttcc atggcaccac
     4621 caggcgcacc ggcgcaccac tttgctttgg caacacctcg ccatacatgc caaaactcag
     4681 cagggtcagt gggtgcatcg cctcatccat gcgcaaggcc tcgacgtagg gccagtccag
     4741 cacccgcgaa ccgacaccgg gcatggtcct ggggtcagca agggtgacaa actcgacgta
     4801 tttggcgctg cccaaaggct ccaccttttt gatcagctcc gatagcgaat agccaaccca
     4861 gggcaacacc atcgaccaac cctcgacaca gcgcaagcgg taaatgcgtt cttcctgcgc
     4921 gctaagcttg atcaggtcct ccagcgtgta gcgggccggt tttttcacca tcccttcaat
     4981 ttccaccgcc catgggctga ttttcagggt gtgggcgttc ttcgccgggt cggccttgtc
     5041 ggtgccaaat tcgtaaaaat tgttgtagct gctggcatcc ttgtagtcgg tcactttgtc
     5101 catcgtgaca ccgcctgcta cgcgggtccg gctaccaggc aaggcgacat tcttgcccgg
     5161 caaggccacc gactgggcaa aagcctgccg tgcgaccagc gagcccagcg ccgcgccagc
     5221 gacgccggtc gccatcaggc gaatcagatc acgtcgggat tgataaatcg caagtggtgt
     5281 gatgctgctg gactcaggat gattgaagcc gtctcgtctg gtgctgatct gcatgttgaa
     5341 gccctcgttt tcatctttgt cgcgcaagca taggcgttct tacaaaaaca tgcgcaaagc
     5401 cggaaaaacg tcgctggcat tgcgcgccgg ccatcttttt caaagttggc aaaaaaaagg
     5461 gccgtaaaca cggcccgatg gattcaaact ggcggtgacc gtagcaaagc caataccgca
     5521 gtttcttgtt aacgaaaagt gatcagcgca agcctgccac gtaatcggca accgccttga
     5581 tctcacggtc gttaaggcgg gctgccacct gtgtcatctg aaggctgttt tgccgaaccc
     5641 catcccgaaa cgccgtcaac tgcgccgccg cgtaatcagc atgttgccca ctcaaacgcg
     5701 gaaactgggc tggaatgccg gcaccagtcg ggctgtggca acctgcgcag gcgggcactt
     5761 tgcggtcggc gatgccgcca cggtaaatgc gctcgcccaa gatcagtgat tccttgttgg
     5821 cggctgaccc tggtgtggat ttcttgctgc taacccagta ggcgatattt ttcatgtctt
     5881 catcgctcaa cggcgctgac atgccggtca tgatggcatt ggcccttttg ccgaccttga
     5941 attcctgcaa ttgcttgacc agatactcgg gatgctgctg cgccaacttg gggttaaccg
     6001 gaataaccga attgccatca ggtccgtgac aggctgcaca aactgcgaaa cttgcttctc
     6061 ccttggccag gtcgtgtttc gctgcgggtg ctgcagcccc ctcggcagag caaacagaca
     6121 ccgtcgcagc catggttgcg gcaatcatga aatgggcaat gagcttcata gtgggtaggt
     6181 caggtcagtg atgctaaacg ggtcgattct acaatgcggc aaatccgcaa tttcaacttg
     6241 atgaccaatc cttccaaacc agccgacttg catgatgccg ggtctgccga gccattgacg
     6301 gccagcatca ccgccccgat ggcgctaggc tggctgcata ctgccaaatt tctcaccacg
     6361 gcaccccaat tgcacttttt accgccgctc agcgtgccgg aaatcgcttt tgtgggtcgc
     6421 tccaacgctg gcaaatcgac ctgcatcaac accctgacgc aacaaaagca gcttgccttt
     6481 gcctccaaaa agcccgggcg aacccagcac atcaacctgt tttcccttgg caagcaaggc
     6541 atcaccgatg ccgtgttggc cgatttgccg ggctatggct atgcggcagt gccccgccaa
     6601 gacaagattc gctggcagca ggtgatggcc aattacctgg tgacccgtga aaacctgaag
     6661 gccatcgtgc tgatgtgtga cccacgtcat ggactcaccg aacttgacga aatcctgctc
     6721 gatgtggtgc gcccacgcgt tgaagaaggc ttgaagtttc tggtggtttt gaccaaggcc
     6781 gataagctga cccgatctga acaagccaag gccctgtcga tcatgaaact gcaggctggt
     6841 ggcggcgagg tgcggctgtt ttcggccacc aagcgccaag gcctggacga agttgccaca
     6901 ctgctgtggc actgggcaca tccggcgcct ctgactgaca ctgaagtag
//
LOCUS       DAOSLL010000185         6947 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9888_length_6947_cov_4.001596, whole genome shotgun sequence.
ACCESSION   DAOSLL010000185 DAOSLL010000000
VERSION     DAOSLL010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6947
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9888_length_6947_cov_4.001596"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="PLB25_09595"
     CDS             complement(<1..466)
                     /locus_tag="PLB25_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3025 domain-containing protein"
                     /protein_id="HOE41866.1"
                     /translation="MGEPVAQQVQAGCAQPQALNRAAQALWPTLGQAPVLFVPQADLP
                     PGKAYECYIFESMQCPTREGLHDFFNGLAWLQFPLTKQRLNQLHVAQIASTGIAPVRG
                     PARDALTLFDENAALLRAPDALWQALVAKDWQRMFGDLRALWDASCLVLFGHA"
     gene            complement(535..1440)
                     /locus_tag="PLB25_09600"
     CDS             complement(535..1440)
                     /locus_tag="PLB25_09600"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOE41867.1"
                     /translation="MNIFFIDADNLNSADWIDEAFRVLQETVGSLSVRRAYGSAESLK
                     ALSEPLQKWAVRPFVNLPLTKNTTDMALAVDAMALACQTPAPRLLVIGSGDADYAPLL
                     VRLRELGIGVICVSERGKMAREAVPAYDRVILVGQATQSADAAAQATEVRAVRKSPRK
                     AAVKTPMTSQVSPEKVQSTKPSTRKTASRKTAQNTTAPGPEVPQEAAISAPAADKPER
                     LAAQVSANQAPVLNVAAILAVVPGLQDGQTLPINVVTKALRDAQLLGKKAASPRLFRN
                     FPDDFELVPAENPNSVRYILPSQVS"
     gene            complement(1468..2520)
                     /gene="pyrC"
                     /locus_tag="PLB25_09605"
     CDS             complement(1468..2520)
                     /gene="pyrC"
                     /locus_tag="PLB25_09605"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOE41868.1"
                     /translation="MIQTLTLTRPDDWHLHLRDGDALAAVLPHTAAQFARAIIMPNLR
                     PPVTSTEQALAYRERIKAALPAGMKFEPLMTLYLTDLLPADEIARAKDAGIVAVKLYP
                     AGATTNSDAGVTDLRKTYKTLEAMQRAGLLLLLHGEVTDPAIDLFDREAVFIDSQLIP
                     LRRDFPELKIVLEHITTREATQYVQASDRFVGATLTAHHLLFNRNAIFTGGIRPHYYC
                     LPVLKRETHRRALLAAATSGNPKYFLGTDSAPHAVHLKEHASGCAGCYTAHTAMAMYA
                     QAFDSVGALDRLEAFASFHGADFYGLPRNTGSITLKRESWTIPERFAFGQADLKPLCG
                     GEVLAWRVQGQEHANL"
     gene            complement(2628..2900)
                     /locus_tag="PLB25_09610"
     CDS             complement(2628..2900)
                     /locus_tag="PLB25_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41869.1"
                     /translation="MLISMRKWWCWLKAVWSGRKPESHERRRALALIAAIDAGGVPLN
                     PARVNDIARGLGLEVSRKAPVESTIARIRRALDFKASGGDDQNPPA"
     gene            complement(2894..3499)
                     /locus_tag="PLB25_09615"
     CDS             complement(2894..3499)
                     /locus_tag="PLB25_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41870.1"
                     /translation="MELLTLLTLLVLGMHVLNTRDQRARVALLGSHLGKYRIENLMES
                     LIQGYLRALGEADLQRQAAIWGMLETTEVELCEQFNAFVLDFATVPEPQARVSKLWLA
                     LPYATRWMPALTFDVRKLLGVHAHAMTHAANKGLPLPHKAKAFTLMAELLLLQHTCHW
                     FCRSKLVASARLLARHQTPYAQVLASVAPQTRQAYCALVGC"
     gene            complement(3744..5543)
                     /locus_tag="PLB25_09620"
     CDS             complement(3744..5543)
                     /locus_tag="PLB25_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HOE41871.1"
                     /translation="MPAGPAGMAVSRSDWATLKRLFPYLWDYKWRVMLALAFMVGAKM
                     ANVGVPLLLKQLVDTMNPKGMVDAQALLVVPLGLLVAYGLLRLSTSVFTELRELIFSK
                     ATEGASRAISLQVFRHLHALSLRFHLERQTGGMTRDIERGTRAVHSLISYSLYSIFPT
                     LIEVIMVLTLLAVKFDIWFAWITVIALVVYISFTIVVTEWRTQFRKQMNELDSTAHSR
                     AIDSLLNYETVKYFNNEEFEARRYDENLVRYRQAAIKSQTSLSLLNTGQQLIIAAGLV
                     AMLWRATQGVVDGRMTLGDLVMVNAFMIQLYIPLGFLGVLYREIKQSLTDLDKMFNLM
                     EREREVADLPGAVPLNVQDAKVQFNHVNFAYDPARPILHDISFEIPAGKTVAVVGPSG
                     SGKSTLARLLFRFYDVSNPADGGQILIAGQDIRQVTQASVRQAIGIVPQDTVLFNDTV
                     EYNIAYGRPGASRAEVVAAAQSAHIHNFIASTPKGYDTMVGERGLKLSGGEKQRVAIA
                     RTLLKNPPILIFDEATSALDSANERAIQAELQSVAQNKTTLVIAHRLSTVVDAHEILV
                     MEAGYILERGTHAALLAANGRYAQMWALQQSSE"
     gene            5628..6080
                     /locus_tag="PLB25_09625"
     CDS             5628..6080
                     /locus_tag="PLB25_09625"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HOE41872.1"
                     /translation="MSANTSPEPDTHTESPDKAPEVRLPLDEELVLKVIPMPRDCNHN
                     GDIFGGWVMAQVDLAGAVIPARYAGGRMATVAVNQFIFKHPVRLGDILSFYSKLVRIG
                     RTSITVKVEVYAEHFRAQGRYTKVTEASLTYVAIDEVGRPRPVPMSAT"
     gene            complement(6517..6858)
                     /locus_tag="PLB25_09630"
     CDS             complement(6517..6858)
                     /locus_tag="PLB25_09630"
                     /inference="COORDINATES: protein motif:HMM:NF012730.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HOE41873.1"
                     /translation="MLSRRDEFVVGFFLMLVMLLIASSLMYLAEHDAQPKLFSSIPAA
                     FWWGVITFTSVGYGDVYPVTTLGRIIGGIFAIVGISVFALPTAILTAAMLDQIHIERK
                     DQQESEKQPPS"
BASE COUNT         1514 a         2040 c         2016 g         1377 t
ORIGIN      
        1 aggcatggcc aaacaacacc aggcatgatg cgtcccagag tgcgcgcagg tcgccaaaca
       61 tccgctgcca gtccttggcc accagcgcct gccacaaggc atctggcgca cgcagcagcg
      121 ctgcgttttc atcaaacaag gtcagggcat cgcgcgccgg gccgcgcact ggcgcaatgc
      181 cggtgctggc aatctgcgcc acatgcaact gattcaggcg ctgtttcgtc agcggaaact
      241 gcagccacgc caatccattg aaaaagtcgt gcaagccctc gcgggtcggg cattgcatgc
      301 tctcaaatat ataacactca taggccttgc cgggtggcag atcagcctgc ggcacaaaca
      361 acaccggggc ttgaccaagt gtcggccaaa gcgcttgcgc ggcccggttg agtgcctgag
      421 gctgcgcgca gcctgcctgc acttgctgcg cgacaggctc tcccattgtg cgccaagggg
      481 tcagccaagc ggcattccag ttgatcgccg ccaaatcagc ggctgacgat gtgctcaaga
      541 tacctgtgaa ggcagtatgt aacgcaccga atttgggttt tcggcaggaa ccagctcgaa
      601 gtcatccgga aaatttctga acaacctggg cgacgcagcc tttttcccga gcaattgcgc
      661 atcacgcaag gcttttgtga ccacattgat gggcagcgtc tgcccgtcct gcaatccggg
      721 gaccacggcc aaaattgccg ctacattcaa aaccggtgct tgattggcgc tgacctgcgc
      781 tgccagtcgc tccggcttgt cagccgctgg cgcactgatg gcggcttctt gtgggacttc
      841 tggcccgggc gctgttgtgt tctgggcagt tttccttgac gcagtctttc tggtcgatgg
      901 cttggtcgat tgcacttttt ctggggaaac ttggctggtc atgggcgttt tgacagccgc
      961 ttttcgggga ctcttgcgca ctgcgcgtac ctcggtggcc tgcgctgccg cgtccgctga
     1021 ctgcgtggcc tgaccgacca gaatcacccg gtcataagct ggcaccgctt cgcgcgccat
     1081 cttgccgcgc tcagacacac aaatcacacc aatgccgagt tcacgcaggc gcaccaacag
     1141 tggcgcatag tcggcgtcac ctgagccgat caccaacagt ctgggcgcag gtgtctgaca
     1201 agcaagcgcc atggcatcga ctgccagcgc catgtcagtg gtattttttg tcagtggcag
     1261 attgacaaag ggtcgcaccg cccacttttg cagcggctca gacagtgctt tgagactctc
     1321 ggcactccca tatgcacggc gcaccgacag cgagcctacg gtttcctgca acacgcgaaa
     1381 cgcctcgtca atccagtccg ctgagttcag gttatccgcg tcaataaaaa aaatattcat
     1441 gtgcgtgttc caggtgggtg cggtgaacta aaggtttgca tgttcttgac cttgcacccg
     1501 ccaggccagc acctcgccac cacacagggg tttgagatcc gcctgaccaa aggcaaagcg
     1561 ctcgggaatg gtccagcttt cgcgcttcaa ggtgatgctg ccggtgttgc gcggcaggcc
     1621 gtaaaagtcg gcaccgtgaa aactggcaaa ggcttcgagc cggtcgagag cgcccaccga
     1681 atcaaacgcc tgcgcgtaca tcgccatggc ggtgtgggcg gtgtagcaac cagcgcagcc
     1741 actggcgtgt tctttcaggt gcaccgcatg gggggcgctg tcggtgccca gaaaatactt
     1801 cggattgccg ctggtcgccg cagccagcag cgcgcggcga tgggtttcgc gtttgagcac
     1861 cggcagacag taatagtgcg gacgaatacc gccagtaaaa atggcgttgc ggttgaacag
     1921 caggtggtgc gcggtgagcg tggcaccgac aaagcggtca gatgcctgca catactgggt
     1981 ggcctcacgc gtggtgatgt gctcaagcac aattttcagt tcgggaaaat cacggcgtag
     2041 cgggatcaac tggctgtcga tgaacacggc ttcgcggtca aacaggtcaa tcgcggggtc
     2101 tgtcacctcg ccatgcagca gcagcagcag gccagcgcgc tgcatggctt ccagggtttt
     2161 gtaggtcttg cgcagatcag tgacaccggc gtcgctgttg gtggtggcac cggccgggta
     2221 cagcttgacc gccacgatgc cagcgtcttt ggcgcgagct atttcatcag caggcagcaa
     2281 gtcagtcagg taaagcgtca tcaatggctc aaacttcatg cccgcaggca gtgccgcctt
     2341 gatgcgctcg cggtaggcca gcgcctgttc ggtgctggtc actggcgggc gcaggttggg
     2401 catgatgatg gcacgggcaa attgggcagc ggtgtggggc agcactgccg ccagggcatc
     2461 gccgtcacgc aaatgcagat gccagtcgtc agggcgggtc agggtaaggg tttggatcat
     2521 ggccggtatt gtcccaaaag tgcacttcac caggtcaagc tggtgccacg ataaaggcca
     2581 gccaacacag tctggcggtg gcatgcagca cctgggctgg ctggccgtca agccggcggg
     2641 ttttgatcat cgcccccaga agccttgaaa tccagcgccc ggcgaatgcg cgcaatggtg
     2701 ctttccaccg gtgcctttct ggacacctcc aggcccagcc cgcgtgcaat gtcattgacg
     2761 cgcgccggat tgagcggcac gccacccgcg tcaatcgcgg caatcaacgc cagcgcgcgc
     2821 cgacgctcgt gggactcggg tttgcgccct gaccatacgg ccttcagcca gcaccaccac
     2881 ttgcgcatcg agatcagcat ccaaccagcg cgcaataggc ctgccgcgtt tgcggtgcca
     2941 ctgaagccag cacctgggcg tagggtgtct ggtggcgcgc cagcagtcgc gccgaagcaa
     3001 ccagtttgga tcggcaaaac caatggcaag tgtgctgcag caacaacaat tcggccatca
     3061 gggtgaatgc cttggccttg tgcggcaatg gcaggccttt gtttgccgca tgggtcatgg
     3121 cgtgggcgtg aacacccagt agcttgcgca catcaaaggt caaggcaggc atccagcggg
     3181 tcgcatacgg cagggcaagc cacagcttgc tgacacgcgc ctgcggctcg ggcaccgtgg
     3241 cgaaatccag cacaaaggca ttgaattgct cgcacaactc aacttcagtg gtctccagca
     3301 tgccccagat ggcagcctgt cgctgcaagt cagcttcgcc cagcgcccgc agataaccct
     3361 gaatcaggct ttccatcaga ttttcaatgc ggtatttgcc cagatggctg cccagcaagg
     3421 caacccgcgc ccgctggtcg cgcgtgttca gcacatgcat gcccagcaca agcagcgtca
     3481 ataaagtcaa gagttccatg gtcggttaca agcgtttcag gtttgcaaca gtgtagtggg
     3541 cgcaacacga cggcaacggt ggcaagtccc gatacattgc gcgactgatt accttcaaga
     3601 gagccagcaa tcgcattgaa tggtctgctt cagatggcgg ccaggctgcc gggagatctc
     3661 gccacagatc aaattcacta ttgtttaaat agctgccagc gcatattgca taagggctac
     3721 agccgtattt tataaaaaaa ttgctactct gacgactgct gcagcgccca catctgcgca
     3781 taccgtccgt tggcagccag cagcgctgca tgggtgccgc gctccagaat atacccagct
     3841 tccatcacca aaatctcatg cgcatcaacc acagtggaca agcggtgcgc gatgaccagc
     3901 gtggtcttgt tttgcgccac gctttgcagc tcagcctgaa tggcccgctc gttggcgctg
     3961 tcgagcgccg aagtggcctc atcaaagatc aggatcggcg ggtttttcaa cagggtgcgg
     4021 gcaatcgcca cgcgctgctt ttcgccgccc gacagcttca gtccgcgctc gccgaccatg
     4081 gtgtcgtagc ccttgggggt ggaggcaatg aaattgtgga tgtgcgctga ctgtgcggct
     4141 gccaccactt cggcccggct ggcaccgggg cggccatagg caatgttgta ttccaccgtg
     4201 tcattgaaca gcaccgtgtc ttgtggcacg atgccaatgg cctgacgcac gctggcctgg
     4261 gtgacttgcc tgatgtcctg cccggcaatc aggatctggc ccccgtcagc cggattgctg
     4321 acgtcataaa accggaacaa cagccgtgcc agggttgact tgcccgagcc cgatgggccc
     4381 accaccgcca cggtcttgcc cgccggaatt tcaaagctga tgtcatgcag gatcggtcgc
     4441 gccgggtcat aggcaaaatt gacatggttg aactgcacct tggcatcttg cacattcagc
     4501 ggcacggcac ccggcaggtc agccacttcg cgttcgcgct ccatcaggtt gaacatcttg
     4561 tccaggtcgg tcaggctctg cttgatctcg cggtacagca cgcccaaaaa acccagtgga
     4621 atatagagct gaatcatgaa agcgttgacc atcaccaggt cgcccagcgt cattcggccg
     4681 tcaaccacgc cttgtgtggc gcgccacaac atggccacca gaccagcggc aatgatgagc
     4741 tgctggccgg tattgagcag actcaggctg gtctggcttt tgatcgcggc ctggcggtaa
     4801 cgcaccaggt tttcgtcgta gcggcgtgcc tcaaactctt cgttattgaa gtacttgacg
     4861 gtctcatagt tcagcagcga atcaatggca cggctgtgag cggtcgagtc cagctcgttc
     4921 atctgctttc tgaactgggt gcgccactcg gtcaccacaa tggtgaagct gatgtaaaca
     4981 accaacgcga tgacggtgat ccaggcaaac cagatgtcaa acttgaccgc cagcagggtc
     5041 agcaccatga tgacttcaat cagcgtcgga aaaatgctgt agagcgagta gctgatcagc
     5101 gaatgcacgg cgcgcgtgcc acgctcgatg tcacgggtca tgccgccagt ctggcgctcc
     5161 aggtgaaagc gcaggctcag ggcgtgcaaa tgacgaaaca cctgcaggct gatggctcgc
     5221 gaagcgccct cggtggcttt ggaaaaaatc agctcacgca gttcggtgaa aaccgaggtt
     5281 gacaggcgca gcaggccata cgccaccagc aggcccagcg gcaccaccag cagcgcttgc
     5341 gcatcgacca tgcctttcgg gttcatggtg tccaccagtt gcttgagcag caatggcacg
     5401 ccgacattgg ccattttggc cccgaccatg aacgccagcg ccagcatcac gcgccatttg
     5461 taatcccaca aataggggaa cagccgcttg agggtggccc agtcagagcg tgaaacggcc
     5521 atgccagcgg gcccggcagg catgtcagaa gatggggtgg aaacagattc gccagaacgg
     5581 cgcatgggtg acaatctcgc taattgaaat caaccccgat tgtgcccatg tctgccaaca
     5641 ccagcccaga acccgacacc cacaccgaaa gcccggacaa agcgcctgaa gtgcggttgc
     5701 cactggacga ggaactggtg cttaaagtca ttccgatgcc gcgtgattgc aatcacaacg
     5761 gcgacatttt tggcggctgg gtgatggccc aggtggacct ggccggtgcg gtgattccag
     5821 cgcgctacgc gggcggacgc atggccaccg tggcggtcaa ccaattcatc ttcaagcacc
     5881 cggtgcgcct tggcgacatt ttgtcgtttt actcgaaact ggtgaggatt ggccgcacct
     5941 ccatcacggt caaggtcgag gtgtatgcgg aacactttcg cgctcaaggc agatacacca
     6001 aagtgaccga agcgtcattg acttacgtgg cgatcgacga ggtcggcagg ccaaggccgg
     6061 tgcccatgtc tgcaacttga aactgggcga taaccagcgt tgttgggtca gccactacag
     6121 acaccggcta cagacggcaa atcagccggg ggcgagcttt cgtgtcgcaa catgtgttga
     6181 aggctctgcc accggtgttg gccataaaag ccaaatcctg accccggcaa atgctggcca
     6241 gttgcatgtt caccaagcat caaacaacgg attgctcacc catcgaaagc gttcttggta
     6301 gagcgcgagc atcacgcctg gcgacaggtg gtactcagaa ctgaatgcca cctgaagcgc
     6361 aaactcaaac ggacgagttt ggctgttctg caccgaactt ggatctctgg caggcagatc
     6421 gaggcgacca acttgcacca gcggagcgtg ttggccgaaa aaaccgtcag tcagccgtca
     6481 atgaatcgcc agttcacatt cagcacgcaa gtccaatcag ctcggaggct gcttttctga
     6541 ctcttgctgg tccttccttt caatgtgaat ttgatccagc atggctgcgg tcaatatcgc
     6601 ggtcggcaat gcaaaaacgc tgattccaac gatggcaaaa atacccccta ttattcgccc
     6661 caaagtcgtg acaggataaa catcaccata tcctacagag gtaaatgtaa tcacacccca
     6721 ccagaaggcc gctggtatgc tcgaaaataa tttgggttga gcgtcgtgtt cggccagata
     6781 catcagagac gatgcaatca aaagcatgac caacatgaga aaaaatccta caacaaactc
     6841 gtctctacgg gaaaggatgg caagtttgat aaactggtat gccgaactgt agcggctcta
     6901 tgccgttttg tgtggaactt gacatcacaa gcagcgatgg actagca
//
LOCUS       DAOSLL010000186         6940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9898_length_6940_cov_4.478141, whole genome shotgun sequence.
ACCESSION   DAOSLL010000186 DAOSLL010000000
VERSION     DAOSLL010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6940
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9898_length_6940_cov_4.478141"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..431
                     /locus_tag="PLB25_09635"
     CDS             12..431
                     /locus_tag="PLB25_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HOE41874.1"
                     /translation="MFEGWFASEADFQTQRSRLMVACPLCGDVNVSKRLSAPRLNLCG
                     NVARDESEEPDIAGVELALAEDLQEAWLAAARQIVAQSEDVGMNFSAEARKIHYGESQ
                     QRAIRGVASPAQAASLIDEGINVVSFALPESFKKTMQ"
     gene            complement(552..677)
                     /locus_tag="PLB25_09640"
     CDS             complement(552..677)
                     /locus_tag="PLB25_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41875.1"
                     /translation="MKPLYSFLLLTLVLGALGAVFALYAQPDFLVMLANQVWSCF"
     gene            complement(805..2025)
                     /locus_tag="PLB25_09645"
     CDS             complement(805..2025)
                     /locus_tag="PLB25_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE41876.1"
                     /translation="MSKLAQSLSIPQVLICGAAIVTVSMGIRHGFGLWLQPITQEQNW
                     SRETFAFALAIQNLVWGFAGIFAGMLADRFGAFRVIVAGAALYALGLLGMAYASTPLM
                     FSLTAGVLIGLAQAGTTYAIVYGVIGRNVPTDQRSMAMGITAAAGSFGQFLMVPTEGF
                     LINSLGWQQALLVLAGASLLMMPIAWWLREPHFQGGTGHAREQSILDALYEAFKYPSF
                     QLLMAGYFVCGFQVVFIGVHMPSYLKDQGLSPQVASYSLALIGLFNVFGSYTTGVLGQ
                     RMQKRYILAFIYLTRAVVISVFLAVPLSPMSVYVFSAAIGLLWLSTVPPTNAAVAQIF
                     GVAHLSMLSGFVFFSHQVGSFLGVWLGGYLFDRFGSYNVVWYISIALGVLAGLLNLPI
                     KEAAIARTSAPQAT"
     gene            2148..4118
                     /locus_tag="PLB25_09650"
     CDS             2148..4118
                     /locus_tag="PLB25_09650"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HOE41877.1"
                     /translation="MATKPNSEYSESSIRVLKGLEPVKQRPGMYTRTDNPMHVIQEVL
                     DNAADEALAGHGKKIKVTVHMDGSVSIEDDGRGIPFGLHPTENAPVIELVFTQLHAGG
                     KFDKGKGGAYSFSGGLHGVGVSVTNALSTRLEVTTYREAQVARLVFSGGDVIEPLTVR
                     PAGDGERRSGTCVRVWPDSKYFESMVLPMTHLEHLLRSKAVLMPGVTVTLLQEKTKES
                     QTWLFKGGLRDYLTQTLAGEPVIPLFEGDGFADAQHESFADGEGATWCVAFTEDGQPV
                     RESYVNLIPTTAGGTHESGLRDGLFAAVKSFVDMHSLLPKGVKLLPDDVFARASYVLS
                     TKVLDPQFQGQIKERLNSRDALRLVSGFVRPALELWLSQNVEYGKKLAEIAIKAAQSR
                     QKAGQKVEKRKGSGVAVLPGKLTDCESRDLAQNEVFLVEGDSAGGSAKMGRDKESQAV
                     LPLRGKVLNTWEVERDRLFANTEIHDISVAIGVDPHGPNDAPDLSGLRYGKICILSDA
                     DVDGSHIQVLLLTLFFKHFPKLIDAGHIYVARPPLFRVDAPARGKKPASKAYALDEGE
                     LTAILDKLRKEGVRESAWTISRFKGLGEMNAEQLWDTTLNPDTRRLLQVGLGNLDTRQ
                     TAELMTKLMGKGEAGARRDLMELHGDSVEVDI"
     gene            4090..4353
                     /locus_tag="PLB25_09655"
     CDS             4090..4353
                     /locus_tag="PLB25_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41878.1"
                     /translation="MAIPLKSIFDYELCSCQRINGKACGLFCIQFDGLESVHSRVNAM
                     GGCLMLDFQKSATAHSADCANLFGAQRSKVRLAGPPIGDLELF"
     gene            4749..>6940
                     /gene="parC"
                     /locus_tag="PLB25_09660"
     CDS             4749..>6940
                     /gene="parC"
                     /locus_tag="PLB25_09660"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HOE41879.1"
                     /translation="MTNPTLFEPPEPLLPEADDSPSLANYAQRAYLEYALSVVKGRAL
                     PDVCDGQKPVQRRILYAMSRMGLGFGGANGNVGAKPVKCARVVGDVLGRYHPHGDSAA
                     YDALVRMAQDFSQRYPLIDGQGNFGSRDGDGAAAMRYTEARLAKITSLLLDEIDEGTV
                     DFSPNYDGSTEEPRQLPARLPFALLNGASGIAVGLATEIPSHNLREVAAACVALIKTP
                     ELSNDELFALMPGPDYPGGGQIISSATEIAEAYRSGRGSLKCRARWKIEDLARGQWQL
                     VVTELPPGVSTQRVLEEIEELSNPKVKTGKKALSLEQNQLKATLLSVLDVVRDESSKD
                     AAVRLVCEPKTSRIGQQELITTLLAHTSLETSSPINLTMIGLDGRPTQKSLRQMFTEW
                     IAFRQTTIERRSRHRLGKVLDRIHILEGRALVLLNIDEVIAIIRQADDPKAALMRRFN
                     LSGAQAEDILEIRLRQLARLEAIKIEQELAGLRVEQQRLEDILSNPVLLRRLMIKEIE
                     ADAKTFADPRRTLIQAEKKAVMEVKVVDEPVTVVVSEKGWVRAQKGWAKDRLGASSTA
                     SSSTAVVEYSFKSGDALYGTFECRSVDTLLAFGSNDRVYSVAVSLLPGARGDGQPMTS
                     LIELEASTQLLYYFAGPVNAGLLISSSGGYGFIAMVENMVSKFKAGKAFVTVAEGETL
                     CPPSVVACDAGAVPLPGATHVACASTGGRILTFEISELKTMSNGGRGLL"
BASE COUNT         1560 a         1951 c         2043 g         1386 t
ORIGIN      
        1 ctgccagcca tttgtttgag ggttggttcg cttctgaggc tgatttccag acacagcgga
       61 gtcgtttgat ggtggcctgc ccgttgtgcg gggatgtcaa tgtcagtaaa cggctgagtg
      121 cgccgcgcct caatctgtgt ggcaatgttg caagagatga atctgaagaa cctgatatag
      181 ccggggtgga gttggcactt gctgaagatc tgcaggaagc gtggttagca gcggcccgtc
      241 agattgttgc gcaatctgag gatgtcggca tgaatttttc agccgaggct agaaaaattc
      301 actatggaga atctcagcaa cgtgcaattc ggggagtagc cagtccggcg caagctgcat
      361 ctctcatcga cgaaggcatc aatgtcgttt cgttcgcttt gcccgaatcg ttcaagaaaa
      421 ccatgcagta gaacgaggtg caagtcgtgt ggcctctgcc attcacacca attgcttgat
      481 tgccagcatc taaccgaaga aacaaaaaac tcaaggcgag taagcggggt tgccagcatg
      541 cccaggcagg attaaaaaca gctccagacc tggttggcca gcatcaccaa aaagtcgggc
      601 tgtgcataca aggcaaacac tgcacccaac gcccccaaca ccagggtcag cagcaaaaaa
      661 ctgtaaagcg gcttcattcc ggcaattctt caagagcgat tattgaccgc aaagaaattt
      721 gataataatc ggctctagcc cttgctgctg taacgctact agctatcatt attgataact
      781 cggtgacgcg tttcaatcgc tgattcacgt cgcttgaggt gccgaagtgc gcgcaatggc
      841 tgcttccttg attggcagat tcagcaagcc agcgagtacc cccagtgcga ttgagatgta
      901 ccagaccacg ttgtagctgc caaaacggtc aaacagataa ccccccagcc agacccccag
      961 aaacgagcca acctggtggc tgaaaaacac aaaaccgctg agcatcgaca agtgcgccac
     1021 gccgaaaatt tgcgctacag ccgcattggt cgggggcacg gttgacaacc acagcagccc
     1081 tattgccgca gaaaacacat aaacactcat gggcgacaac ggcactgcca aaaacacact
     1141 gatcaccacg gcacgggtca ggtagataaa cgccaggatg tacctttttt gcatccgttg
     1201 ccccaagaca cctgttgtat aactgccaaa aacattgaac aggccaatca gcgctagcga
     1261 ataacttgcc acctggggcg acaaaccctg atccttaaga tagctcggca tgtgcacacc
     1321 aatgaatacc acctgaaaac cgcaaacaaa atagccagcc atcagtaact ggaaactggg
     1381 gtacttgaag gcttcgtaca aggcatccag aatggactgc tcacgagcat gcccggtgcc
     1441 accttggaaa tgcggttcgc gcagccacca ggctatcggc atcatcagca gcgaggcacc
     1501 agccagcacc aacagagcct gctgccagcc caggctgttg atcaaaaagc cctcggttgg
     1561 caccatgaga aactggccaa acgaaccggc cgctgcggtg atgcccatcg ccatggagcg
     1621 ctgatcggtc ggcacattgc ggccaatgac gccatacacg atcgcataag tggtccccgc
     1681 ctgggccagt ccgatcagca ccccggcggt caatgagaac atcagcgggg tgctggcata
     1741 cgccatgccc agcagtccca gcgcatagag tgcagctcct gccacgatca cccgaaatgc
     1801 accaaagcgg tcggccagca tgccggcaaa aatgccagca aacccccaca ccagattctg
     1861 gattgccagc gcaaaggcaa aggtctcgcg gctccagttt tgctcctggg tgatcggctg
     1921 caaccacagg ccaaagccat ggcggatgcc catggacacc gtgacgatgg cggcaccgca
     1981 aatgaggact tgcgggatgg agagggattg ggcaagtttt gacatttgtg gaatatagcg
     2041 aatcatgcca attgatgctg gcaagcggtc agatgcattc tgttgtggtg ctggcatctg
     2101 gtgttatgta cagtattttg tccgttgcat gattacaatg cgccgctatg gcgaccaaac
     2161 caaactccga gtattccgaa agttccatcc gcgtgctcaa gggcctggag cccgtcaagc
     2221 agcgcccggg catgtatacc cgcactgaca atccgatgca cgtgatccag gaagtgctcg
     2281 acaacgcggc cgatgaagcg ctggccgggc acggcaaaaa gatcaaggtg acagttcata
     2341 tggacggctc ggtcagcatt gaagacgacg gccgcggcat tccgtttggc ctgcacccga
     2401 cagaaaatgc cccggttatc gaactggtct ttacccaatt gcacgccggt ggcaagtttg
     2461 acaagggcaa gggtggcgcc tacagctttt cgggtggcct gcatggtgtt ggtgtcagcg
     2521 tcaccaatgc gctttcgacc cggctcgaag tcaccaccta ccgcgaggcg caagtggcca
     2581 ggctggtgtt ttcgggcggt gacgtgatcg agcccttgac cgtgcgacca gccggcgatg
     2641 gcgagcgccg ctctgggacc tgcgtgcggg tctggcccga ttccaagtat tttgaatcaa
     2701 tggtgttgcc gatgacacat ctggaacatc tgctgcgcag caaggcggtg ctgatgccgg
     2761 gtgtcactgt gacgctgctg caggaaaaga ccaaagagag ccaaacctgg ctgttcaaag
     2821 gtggcctgcg tgactacctg acccaaaccc tggcgggcga gccggtgatt ccgctctttg
     2881 aaggcgatgg ttttgccgat gcgcagcacg agagttttgc cgacggcgag ggtgccacct
     2941 ggtgtgttgc gtttaccgaa gacggccagc cggtgcgtga gagctacgtc aacctgatcc
     3001 cgaccaccgc tggtggcacc catgaatcgg gcttgcgcga cggcctgttt gccgcggtca
     3061 agagttttgt tgacatgcac tcactgctgc ccaagggggt caagctgctg cccgacgacg
     3121 tatttgcccg tgccagctat gtgctgtcca ccaaggtgct ggacccgcag tttcagggtc
     3181 agatcaagga acggctcaat tcgcgcgatg cgctgcggct ggtgtcgggt tttgtgcgcc
     3241 ctgcgctgga gttgtggctc agccagaacg tcgagtacgg caagaagctg gccgagatcg
     3301 ccatcaaggc agcccagtca cgccaaaagg cggggcaaaa agtggaaaag cgcaaaggct
     3361 caggcgtggc ggtgctgccg ggcaagttga ctgactgcga aagtcgcgat ctggcgcaaa
     3421 acgaggtgtt tttggttgaa ggtgattcag ccggcggcag cgccaaaatg ggccgcgaca
     3481 aggaaagcca ggcggtcttg ccgctgcgtg gcaaggtttt gaacacctgg gaggtcgaac
     3541 gggaccggct ctttgccaac accgagattc acgatatttc ggtagcgatc ggggtggacc
     3601 cgcatggccc caacgatgcg cccgacctga gcgggctgcg ctacggcaag atctgcatcc
     3661 tgagcgatgc cgacgtggac ggctcacata ttcaggtgct gttgctcacg ctgtttttca
     3721 aacacttccc caaactgatt gatgccggcc acatctatgt ggcgcgccct ccgcttttcc
     3781 gggtcgatgc gccagcgcgc ggcaaaaagc cggcgtccaa agcctacgcg cttgacgaag
     3841 gtgaactgac cgccattttg gataagttgc gcaaggaagg cgtgcgtgaa agcgcctgga
     3901 ccatcagccg cttcaagggc ctcggtgaaa tgaacgccga gcagttgtgg gacaccacgc
     3961 tgaaccccga cacacgccgc ctgctgcagg tcggcttggg caatctggac acgcgtcaga
     4021 ccgccgagct gatgaccaag ctgatgggca agggcgaggc cggggcgcgg cgtgacctga
     4081 tggagttgca tggcgattcc gttgaagtcg atatttgact atgaattatg tagctgtcag
     4141 cgcattaatg gtaaggcctg cggcctgttt tgtattcaat ttgatggtct tgagagtgtg
     4201 cacagcagag taaatgcaat gggcggctgc ctcatgctgg atttccagaa gtcggcgact
     4261 gcccatagcg ctgactgtgc gaacctgttt ggtgcgcaaa gatccaaagt caggcttgct
     4321 gggccaccca ttggtgacct tgagctgttt tgaaattttg ataacgctta ttttttcaag
     4381 attcactttt tgccgtggga aaagaggtga tcttcagact gggacagtac cattccattt
     4441 cgcgccagat caggaagtcg gaaattgaag gtaaaacaat ccagggcagt gacgctggtc
     4501 ttgatggccg cggtggactc gacgctgcca gcgaagtggg tggctgtggc ggccgccgat
     4561 ttctggtacc ccagcatgag gcgcccgcca cagccacccg cttcgcggat tcgggccgca
     4621 gccgtcgatt ccgccaaact gtctcgggac gtttttctgc cccctgttta tatacaaaat
     4681 caccctctaa ccaccgtcct tattgcgcaa gcagctatag gtaatatagc aaaattcaca
     4741 acttattcat gactaaccca acactttttg aaccacccga acccctgttg ccagaggccg
     4801 acgacagccc tagcctggcc aattacgccc agcgtgccta ccttgagtac gcgctaagcg
     4861 tggtcaaggg cagggcgctg cctgatgtgt gtgacggcca gaaaccagtg cagcgacgca
     4921 ttttgtacgc catgagccgc atgggcctgg gctttggcgg ggccaatggc aatgtcggtg
     4981 ccaagccggt caagtgcgcc cgggtggtcg gtgacgtgct gggccgctat cacccgcacg
     5041 gcgacagtgc cgcttacgac gcgctggtgc gcatggcaca agacttcagc cagcgctacc
     5101 cgctgattga cggccagggc aactttggca gccgcgacgg tgacggcgct gcggcaatgc
     5161 gttacaccga agcccgcctg gccaaaatca ccagcctgct gctcgacgaa atcgacgagg
     5221 gcacggttga tttttcgccc aattacgacg gctctaccga agagccgcgc cagttgccag
     5281 cgcgcctgcc gtttgcgctg ctcaacgggg ccagcggcat tgcagtcggg ctggcgaccg
     5341 aaatccccag ccacaacctg cgcgaagtgg ccgctgcctg cgtggcactg atcaaaacac
     5401 ccgaactctc caatgacgaa ctgtttgcgt tgatgcccgg gcctgactac ccaggtggtg
     5461 gccagatcat cagcagcgcg accgagattg ccgaggctta ccgcagcggt cgtggttcgc
     5521 tcaaatgccg cgcgcgctgg aagattgaag accttgcgcg cggccagtgg caactggtgg
     5581 taactgaatt gccgcccggt gtcagcaccc agcgggtgct cgaagaaatc gaagaactca
     5641 gcaacccaaa ggtcaagacc ggcaaaaagg ccttgagtct ggagcaaaac cagctcaagg
     5701 cgaccttgct gtcggtgctt gacgtggtgc gcgacgagtc cagcaaggat gccgccgtgc
     5761 gcctggtgtg cgaacccaaa accagccgca tcggccagca agagttgatc accaccttgc
     5821 tggcgcacac cagtcttgag acctcaagcc cgatcaatct gacgatgatt ggcctggacg
     5881 gtcgtcccac ccaaaaatcg ctgcgtcaga tgttcactga gtggattgct tttcgtcaaa
     5941 ccaccatcga gcggcgctcg cgccaccggc tcggcaaggt gctggaccgc atccacatcc
     6001 tggagggccg ggcgctggtg ctgctcaaca ttgacgaggt gatcgcgatc atccgtcagg
     6061 ccgatgaccc gaaggcggcg ttgatgcgcc gcttcaacct gtctggtgcc caagccgagg
     6121 acattctgga aatccggttg cgccagttgg cgcggcttga agccatcaag attgagcaag
     6181 agctggcggg tttgcgcgtt gaacagcaaa ggcttgaaga cattctgtcc aacccggtcc
     6241 tgcttcgccg cctgatgatc aaggagattg aggctgatgc caagactttt gccgaccccc
     6301 gtcgcaccct gattcaggct gagaaaaagg cggtgatgga agtcaaggtg gtcgatgagc
     6361 cggttaccgt ggtggtcagc gaaaaaggct gggtccgggc acaaaaaggc tgggccaaag
     6421 accgccttgg cgccagcagc acggccagca gcagtaccgc agtggtggag tacagcttca
     6481 agtccggcga tgcgctgtat ggcacgtttg aatgccgcag cgtcgatacc ctgctggcct
     6541 ttggcagcaa tgaccgggtt tattcagtgg cagtgtcgct gttgcctggt gctcgtggtg
     6601 atggccagcc catgaccagt ttgattgaac tcgaagccag cacccagttg ctgtattact
     6661 ttgccggtcc ggtcaatgcc ggtttgctga tctcaagcag tggcggttat ggttttattg
     6721 caatggtcga aaacatggtc tccaaattca aggctggcaa ggcttttgtc actgtggccg
     6781 aaggcgaaac cctgtgcccg ccctcggtgg tggcctgtga tgcgggtgcc gtgccgcttc
     6841 caggcgcgac ccacgtggcc tgcgcatcaa ccggtgggcg aattttgacc tttgagatca
     6901 gtgaacttaa aaccatgtcc aatggcgggc gcggcctgct
//
LOCUS       DAOSLL010000187         6932 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9916_length_6932_cov_5.129271, whole genome shotgun sequence.
ACCESSION   DAOSLL010000187 DAOSLL010000000
VERSION     DAOSLL010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6932)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6932)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6932
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9916_length_6932_cov_5.129271"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(276..578)
                     /gene="cowN"
                     /locus_tag="PLB25_09665"
     CDS             complement(276..578)
                     /gene="cowN"
                     /locus_tag="PLB25_09665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(2)-fixation sustaining protein CowN"
                     /protein_id="HOE41880.1"
                     /translation="MGETGITAPAAPAADRYRSFKGIDFDGQASRMMARITSHTQGGD
                     DRFWAYFFDRRQARSGMRCDDLLLLASFVNQIRELLEARGDEIALAWLDQLEQECF"
     gene            complement(580..831)
                     /locus_tag="PLB25_09670"
     CDS             complement(580..831)
                     /locus_tag="PLB25_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOE41881.1"
                     /translation="MPIYDYQCQACGHPFELLVRADSTPACPNCGSTALKKCISSIAP
                     AGKIEAIRMAHRRVAAAQGHFDHYSPSDKAKLLQGKKNI"
     gene            complement(1557..3266)
                     /locus_tag="PLB25_09675"
     CDS             complement(1557..3266)
                     /locus_tag="PLB25_09675"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HOE41882.1"
                     /translation="MHPSSAILLMFRRMRAPLIVLILIYAVSVLGLTLIPGADANGKP
                     WQMGFFHAFYFISYTATTIGFGEIPYAFTDAQRMWVIVSIYLSVIGWAYAIGTLLALV
                     QDRAFQQAITLQRFSYRVRHLHEPFYLVCGYGETGQLLCRAFGDMNRRFVVIDIETSR
                     IDELDLQDLRADAPGLAADARQPDNLLQAGLMSRHCVGVIALTSDDEANLAVAAATRL
                     LQRDMPILCRVTRPEIAANMRSFGIHSIINPFETFGEYLALAIRSPGSYQLVEWLTAT
                     PGSTLETQREPPRGHWILCGYGRFGQAIVDDLQGEGIDLTVIEPNRSITGSVPQVTGV
                     GNERQVLEQAGIAQAVGIIAGTSNDIANLAIVATAEELNPALFTVLRQNQQSNSLLFS
                     TFKADFMVRPAEIIAHECLGLLTTPLLARFLAEVKRQGDAWSDALVARIVQTCGKRVP
                     ATWNVAINAKGAPALLGELGNGTRVKLGALLRDPADRQQSLRVVPLLLLRAGEQHHLP
                     ADDFDLCAGDDILFASTRSSRSLLLGTLVNVNDLGYVLSGRQQGAGWLWRRFARDNSN
                     GSA"
     gene            complement(3282..3665)
                     /locus_tag="PLB25_09680"
     CDS             complement(3282..3665)
                     /locus_tag="PLB25_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020654861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6394 family protein"
                     /protein_id="HOE41883.1"
                     /translation="MNLEKVIFGFFIVFAATLNFGFVIGEIDNPTHHNALELFLAIVV
                     NLIATVLKFGDRTQVGAVHLATSLVADLQLLAAACVWAYAAYASASGVDAYAITTIVS
                     LSAGALLANIVSVVMLIIETMMLRR"
     gene            complement(4062..4901)
                     /gene="metF"
                     /locus_tag="PLB25_09685"
     CDS             complement(4062..4901)
                     /gene="metF"
                     /locus_tag="PLB25_09685"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HOE41884.1"
                     /translation="MTESSRFPLSIEFFPPKTPEGIEKLRLARQQLYALKPEFCSVTF
                     GAGGSTQEGTFSTVRDILSEGVSAASHFSCIGASLGGVREQLAILQAMGVKRLVALRG
                     DLPSGYGAGGEFQYASDLVAFIRREAGNTFHIEVAAYPEMHPQARSPESDLQAFATKV
                     RAGANSAITQYFFNPDAYFRFLEDVDALGVTVPVVPGIMPIASSTQLLRFSDACGAEI
                     PRWIRLRLQAFGDDSASIKAFGLDVVTSLCEQLRAGGAPGLHFYSMNQSAPVLEICRR
                     LGL"
     gene            complement(5137..6000)
                     /locus_tag="PLB25_09690"
     CDS             complement(5137..6000)
                     /locus_tag="PLB25_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HOE41885.1"
                     /translation="MRIDMLLVQRQLASTRSQAQRLIAGGVLWRQGDAWRKIAKNGDD
                     VPDDAQIRLLDDSEARYVSRGGLKLEAALQHTGLSVAGLQCLDVGQSTGGFTDCLLQQ
                     GATLVVGVDVGSAQLHPSLRADPRVLCVEGLNARTLDASKLVAAYADSISASGQFDLE
                     NGNEAEHFEAGDEEGMPLADPSFVPEFDLIVADLSFISQTLVLPAVVPMLKVGGRLLT
                     LVKPQFELQPGQVGKGGIVKDATLYALVERRLRDACAALGLTVSDWFESPIAGGDGNR
                     EFFISAGNNAT"
     gene            complement(6007..>6932)
                     /locus_tag="PLB25_09695"
     CDS             complement(6007..>6932)
                     /locus_tag="PLB25_09695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HOE41886.1"
                     /translation="AAIKAKLAQDPTWYSRRLKNIIGVTEETTTGVHRLNEMSAKGEL
                     MLRAINVNDSVTKSKFDNLYGCRESLVDAIKRATDVMIAGKVACVVGYGDVGKGSAQA
                     LRALSAQVWVVEIDPINALQAAMEGYKVVTVEYAADKCDIFVTCTGNKNVITYKHMEA
                     MKDQAIVCNIGHFDNEIDVASLEKCKWEEIKPQVDHVIFPAKGKKPAKRIILLAKGRL
                     VNLGCGTGHPSFVMSSSFANQTIAQIELFTKPKSYKSGQVYVLPKLLDEKVARLHLKK
                     VGAMLSELTDEQAAYIGVDKAGPYKADTYRY"
BASE COUNT         1473 a         2027 c         2073 g         1359 t
ORIGIN      
        1 tcgttataac ctgcagcaaa taacgtgccg attttttggg tgcggagcca gcaacaaaaa
       61 accatgcggg caggctcgat gggcgggatt cttgaaaagg gcagcgccgc gatgcgaacg
      121 atctgtcgct ggcaagttct ttcaaacgct atataaaagt tagctgcttg cgcttattag
      181 acgggcgcta caggctgttt tggcttatga atgacgatcc gttggcggcc cctgcggcga
      241 ggggctccag tttgaaccct gatgttcacg ccgcatcaaa agcactcctg ttccagttga
      301 tccagccagg ccagcgcgat ttcgtcgccg cgcgcctcaa gcaactcgcg gatttgattg
      361 acaaagctgg ccagcagcag caagtcatca cagcgcatgc ccgacctggc ctgccgacgg
      421 tcaaagaaat aggcccagaa acggtcatcg ccaccctggg tgtgagaggt gatgcgcgcc
      481 atcattcgac tggcctggcc gtcaaagtcg atgcccttga aactgcggta gcggtcagcc
      541 gctggcgctg caggggcggt gatgccggtt tcgcccacgt caaatgttct tcttgccctg
      601 cagcagcttg gccttgtcgg acgggctgta gtggtcaaag tgcccctgcg cggcggcaac
      661 acggcggtgt gccatgcgga tggcctcgat cttgccagcc ggggcgatgc tggagatgca
      721 ctttttcagc gccgtgctgc cgcagttcgg acaagccggg gtgctgtcgg cgcgcaccag
      781 cagctcgaac gggtggccgc aggcctgaca ttggtagtcg taaatgggca tggtggtttc
      841 caaagggttc agtccgggcc aacacaacgc ctggcaggcg atgtgcagcc ttctagtgtc
      901 aggactgacg cagggttcgt gccaggactt ttccagaaca cctgcaggca gcaaccgcaa
      961 agcagtcaaa accggcagcc cgcgtccggc tgtcactgcg ctgcatcagc aatctcgctc
     1021 tgaggctttc ccctttcaaa ggggttatgc catttccata tcaatcctaa gccagacaag
     1081 ctggaatcga acagccttcg ccgcgagggc gcgactccta cacaaacagg cggcatccat
     1141 ctgaatttgt aggagtcgcg ccctcgcgac gaatgacttc atgactttct cgaattcttc
     1201 tcggaaattg caaagacttg acctgttagt gacaaatagg gaattgattc atgatcatgt
     1261 ccttggcctt gcggtgctgt ggcactgtct gcagcttcgc gccaaatgtc gcatcgatct
     1321 ggcaacgcaa ttcgatcaac tttcgccgat ccagccaggc atttcagcgc tgcccagttg
     1381 agcgctgacg atatgccatt catagctgcc agcgcagtac agataagggt tggaggctgt
     1441 ttctatccct gttggggaga atggctgcag agccgggcca gcgccgcggc ttgggtaggc
     1501 ggctacctgc accgatgtgt cggcgcttgt gccacggtgg cgcagccacc aatgcttcag
     1561 gccgacccgt tgctgttgtc gcgggcaaac ctgcgccata gccagcctgc accctgctgc
     1621 cgaccagaga gcacgtagcc aaggtcgttg acgttgacca gcgtgcccag cagcagcgag
     1681 cggctgctgc gcgtgctggc gaaaagaatg tcgtcaccgg cgcacaaatc gaagtcgtcg
     1741 gctggcagat gatgctgttc gccagcccgc agcaacagca gcggcaccac ccgcaaagac
     1801 tgctggcggt cggccgggtc gcgcagcagc gcgccaagtt tgacccttgt gccgttgccc
     1861 agttcgccca gcagcgccgg ggcacctttg gcgttgatcg ccacattcca ggtggcaggc
     1921 acccgcttgc cgcaagtttg cacaatgcgc gccaccagcg cgtcgctcca ggcatcgccc
     1981 tggcgcttga cttcggccag aaagcgcgcc agcagcggtg tcgtcagcag gcccaggcat
     2041 tcgtgggcaa tgatctcggc cgggcgcacc atgaaatcgg ccttgaaggt cgagaacagc
     2101 aggctgttgg actgctggtt ttgccgcagc acggtaaaca gcgccggatt gagttcctcg
     2161 gcggtggcga cgatggccag gttggcaatg tcgttgctgg tgccagcaat gatgccgacg
     2221 gcttgcgcga tgccggcctg ttccagcacc tggcgctcgt tgccgacacc ggtcacctgc
     2281 ggcacgctgc cggtgattga ccggttgggt tcgatcacgg tcaggtcaat gccttcgccc
     2341 tgaaggtcat cgacgatggc ctggccaaac cggccataac cgcacagtat ccagtggccg
     2401 cgcggtggct cgcgttgcgt ttcgagcgtg ctgcccgggg tcgcggtcag ccattccacc
     2461 aactggtagg aacccggcga gcgaattgcc agcgccaggt attcgccaaa cgtctcaaac
     2521 gggttgatga tgctgtggat gccgaacgac ctcatgttgg ccgcgatttc ggggcgtgtc
     2581 acccggcaca gaatcggcat gtcgcgctgc agcaggcggg tcgctgccgc gacggccagg
     2641 ttggcttcgt cgtcgctggt cagcgcaatc acgccgacgc aatgccggct catcaacccg
     2701 gcttgcagca ggttgtcggg ttgccgcgca tcggcagcca ggccgggcgc atcggcgcgc
     2761 aggtcttgca aatcgagttc gtcaatgcgg ctggtttcga tgtcgatcac aacaaagcgc
     2821 cggttcatgt cgccaaaggc gcggcacagc aattgcccgg tttcgccgta accgcagacc
     2881 aggtaaaacg gctcgtgcag gtggcgcaca cggtacgaaa agcgctgcag cgtgatggct
     2941 tgctgaaaag cacggtcttg caccagtgcc agaagggtac caatggcata ggcccagccg
     3001 atcaccgaca ggtagatgct gacgatgacc cacatccgct gggcatcggt aaaggcgtag
     3061 ggaatctcgc caaaaccgat ggtggtggcg gtatagctga tgaaatagaa ggcgtgaaaa
     3121 aaccccatct gccagggctt gccgttggca tctgcgccgg gaatcagggt caggcccagc
     3181 accgacaccg cgtagatcag gatcagcacg atcagcggcg cccgcatgcg ccgaaacatc
     3241 agcaaaatgg cgctgctcgg atgcatcgca ggccagccgg ttcagcggcg cagcatcatg
     3301 gtttcgatga tcagcatgac gaccgagaca atgttggcca gcagtgcacc ggccgacagt
     3361 gaaacgatgg tggtgattgc gtaggcatcg acaccgctgg ctgaagcata ggcagcgtag
     3421 gcccagacgc aggcggcagc gagcagttgc aggtcggcca ccaggctggt ggccagatgt
     3481 accgcgccga cctgggtgcg gtcgccaaac ttgagcacgg tggcgatcag gttgacgacg
     3541 atggccagga acagttcgag tgcattgtgg tgtgtcgggt tgtcgatttc accgatgaca
     3601 aagccaaagt tcagcgttgc ggcaaagacg atgaagaaac cgaagatgac tttttcaagg
     3661 ttcatgaatg agcccggctg gagtggatgg cagcaaattc taagccagcc ggtcgcgaaa
     3721 atgggcatca cggggaccaa tcagacgctg gtctgtcagt ggctctggac ccagctcagg
     3781 gccacacgcc tggtttgtat atgctacatt aaatatagct accagcgcaa cacaggtatg
     3841 ggctagaggc caatttgaac cctatttttt ccctggttga cggattggac gacgatcttg
     3901 cacgggccag caataggaag acgcgtcttg gggagaaaag tgccaatgca aaaaacacgc
     3961 aacgggcaaa gtgacgttac cagacagcca gtgatacccg atgaattgca cgtcctttgg
     4021 gccagacctg gcccgccagg gaaccagccg gctacaacaa actacaagcc cagccgtcgg
     4081 caaatctcca gcaccggcgc actctggttc atgctgtaaa agtgcaggcc gggcgcgccg
     4141 ccagcccgca gttgctcaca caaactcgtc accacatcga gcccaaaggc cttgatcgac
     4201 gcgctgtcgt cgccaaaagc ctgcagccgc agccgtatcc agcgcggaat ttcggcaccg
     4261 caggcatcgc tgaaacgcag caactgcgtt gaactggcaa tcggcatgat gccgggcacc
     4321 accggcaccg tcacgccgag cgcatccacg tcttcgagga acctgaagta ggcatcaggg
     4381 ttgaagaaat actgggtgat ggctgaattg gcaccggcgc gcaccttggt ggcaaaggct
     4441 tgcagatcac tctcgggcga gcgcgcctgc gggtgcatct cggggtaggc ggcgacttca
     4501 atgtggaagg tgttgccggc ctcgcggcgg ataaacgcca ccagatcgct ggcatactga
     4561 aattcaccgc cagccccgta gccgctgggc aaatcaccac gcaatgccac caggcgcttt
     4621 acacccatgg cctgaagtat ggcgagctgt tcgcgcacgc cgcccaggct ggccccgatg
     4681 caggagaaat gcgatgccgc gctgacacct tcactcaaaa tatcgcgcac ggtgctgaaa
     4741 gtgccttctt gggtggagcc gccagcacca aacgtgaccg aacaaaactc cggcttgagg
     4801 gcgtacaact gctggcgcgc cagccgcagc ttttcgatgc cctcaggggt tttgggcggg
     4861 aaaaattcaa tgctcagggg aaaacggctt gactcggtca tgacgagctt tctgaaaaac
     4921 taaatggcat aacgggctta ttgcggcatc agtgcctgcc tgaccacagg tcgtgccatt
     4981 tctggatcat ccaggtgctg gtgatctggc cttgccctgc cagtcggcag ggatgcagcg
     5041 gcacagttgt ttgcaatcgc cgagacttga tgcctgcaga tgaatgattc aggaatggaa
     5101 taacgaatcg aataaggaat cgaataagga acgcaatcag gtcgcattgt tgccagccga
     5161 gatgaagaat tcgcggttgc cgtcgccacc agcaatcggc gactcaaacc agtcggacac
     5221 cgtcagaccc aaagcggcac aagcatcgcg cagacgacgt tcgaccaagg cgtacagtgt
     5281 tgcgtccttg acaatgccgc ccttgccaac ttggccgggt tgcagttcaa actgcggctt
     5341 gacaagggtc agcaagcggc cgccgacttt caacatcggc accacggcag gcaaaaccag
     5401 ggtttgcgaa ataaacgaca ggtcggccac gatcaggtcg aattcaggca caaacgatgg
     5461 gtctgccagt ggcatgcctt cttcgtcgcc cgcctcgaaa tgctctgcct cattcccatt
     5521 ttcaaggtca aattggccgc tagcgcttat gctgtctgcg taagcagcta ctaactttga
     5581 agcatccaga gtgcgtgcgt tgaggccttc gacgcacaag acgcgcggat cagcacgcag
     5641 gctggggtgc aattgggcgc tgcccacatc gaccccgacc accaaagtcg cgccttgctg
     5701 cagcaggcag tcggtaaaac cgccagtgga ctggccgaca tccaggcatt gcaggccagc
     5761 caccgacaag ccggtatgtt gcaaggcggc ctcaagcttc aaaccgccgc gcgatacata
     5821 gcgcgcctcg gaatcgtcaa gtagccgaat ctgcgcatcg tccggtacat catcaccgtt
     5881 cttggcaatt tttcgccagg catcgccttg ccgccacagc acgccaccgg caatcaggcg
     5941 ctgcgcttgc gaacgggtgc tggcaagctg gcgttgcacc agcaacatgt caattcgcac
     6001 cctggctcag tagcggtaag tgtcggcctt gtacggaccg gccttgtcaa cgccaatgta
     6061 ggcagcctgc tcgtcagtga gttcactgag catggcaccg acttttttca gatgcaagcg
     6121 ggccactttt tcatcaagca gtttgggcaa cacatacacc tggcccgact tgtaggactt
     6181 gggcttggtg aacaactcga tctgggcgat ggtctggttg gcaaagctcg aactcatgac
     6241 aaaactgggg tgaccggtac cgcaaccgag gttcaccaga cggcccttgg ccagcaggat
     6301 gatgcgcttg gccggctttt tgcccttggc cgggaaaatg acatggtcaa cttgcggttt
     6361 gatctcttcc cacttgcact tttccagcga ggcgacatca atttcattgt cgaaatggcc
     6421 gatgttgcaa acgatggcct gatctttcat ggcctccatg tgtttgtagg tgatgacgtt
     6481 cttgttgccg gtgcaggtga caaaaatatc gcatttgtcg gcagcgtatt ccaccgtcac
     6541 caccttgtag ccttccatcg cggcttgcag tgcgttgatc gggtcaatct cgaccaccca
     6601 gacctgcgcc gacagggcgc gcagggcctg cgccgaaccc ttgccgacat caccgtaacc
     6661 gaccacgcag gcgactttgc cggcgatcat cacatcggtg gcgcgcttga tggcatcaac
     6721 cagcgattca cggcaaccat acaggttgtc gaacttgctt ttggtgaccg agtcattgac
     6781 attgatggcg cgcagcatca gctcgccctt tgccgacatt tcgttgaggc ggtgcacacc
     6841 ggtggtggtc tcttcggtca caccgatgat gtttttcagg cggcggctat accaggtggg
     6901 gtcctgggcc aacttggcct tgatggcagc gt
//
LOCUS       DAOSLL010000188         6904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_9966_length_6904_cov_4.145861, whole genome shotgun sequence.
ACCESSION   DAOSLL010000188 DAOSLL010000000
VERSION     DAOSLL010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6904
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9966_length_6904_cov_4.145861"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="PLB25_09700"
     CDS             complement(<1..299)
                     /locus_tag="PLB25_09700"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204029.1"
                     /note="NADP-dependent; catalyzes the oxidative
                     decarboxylation of malate to form pyruvate; decarboxylates
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOE41887.1"
                     /translation="MSNPLSPSEQALRDATAEYHRTPTRGKISVTATKPLNNQRDLSL
                     AYSPGVAYPCLEIQANPAMAHEYTSRGNLVGVITNGTAVLGLGDIGPLAGKPVMEG"
     gene            complement(368..1600)
                     /locus_tag="PLB25_09705"
     CDS             complement(368..1600)
                     /locus_tag="PLB25_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006218138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HOE41888.1"
                     /translation="MTPYAQLDAWIDAHFDEQVAFLQQLIRVPTDTPPGNNAPHAERA
                     ADLLADMGLPAEKHPVPAAEVQAAGLQSITNLIVRRHYGPGGKTIALNAHGDVVPPGE
                     GWTHDPYGGEIEDGKIYGRASAVSKCDFSTYSFAIRALEAVAKPRQGQIDLLLTYDEE
                     FGGEVGPGWLLKHKLTQPDLMIAAGFSYQVITAHNGCLQLEVTVHGQMAHAAIPESGV
                     DALQAAVKILNALYAQNTLYQSITSEVEGINHPYLNVGLIEGGTNTNVIPGRVSFKLD
                     RRMIPEENAAQVEATLRELIAATAAGQPGIRIDVRRILLAHSMKPLAGNQPLVDAIQK
                     HGQAVFGEPIAALGTPLYTDVRLFCEAGIPGVIYGAGPRTVLESHAKRADERLDLADL
                     RRATQVMARSLHDLLHRA"
     gene            complement(2215..6870)
                     /locus_tag="PLB25_09710"
     CDS             complement(2215..6870)
                     /locus_tag="PLB25_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE41889.1"
                     /translation="MFKLAATKEDQIVGKTDYDFVDPAQADFFRANDRMAASAGQPTC
                     NEEWLTMAVGGHSGLYETTKMPLVEATGRVIGVLGVSHEITQQRATQHELEERIKEQR
                     CLYDIFTLTQDDHGDFDTQLQAVVERLPAGWQFPEQVAARITLPHRHIAGANYQAGHP
                     SQRAPLPIDGVEVGSIEIAYRDEPRTGHHLVFLSQEQTLLETIAARIAGRMNERQVRQ
                     TLREREAIFAAMTRRAADSMGLVDFDTGRFVEFNDAAAARLGYSREEFAALGVASIEA
                     DQNPAQVQQSFARIATSGSAVFESRHRHKDGSLRDVRVSVSRIEVAGREYLASTWSDI
                     TEEKRIREEIAVRQALYHSVVTSMNEGIIVLDGGGIIRACNPAAERILGVAELDLLGR
                     SGVDASWRAVREDGSSFPAEELPTSRALQSGQAQRDVVLGLPGDNGQMVWISINAQVL
                     PGGSGQGPAGVVTSFTDMTGRKLAEEQLKNSERDLREAQAVARVGSWSLTVQTGELQG
                     SDEAYRIFGLSRHEPISLRKFVASVHPNDLDWMRREWLTAQQGGDFDVEHRIVIGASA
                     QLPEADIRWVRERAEIRRDASGAPVFALGTVQDITERKQMELELILHRDKLEHLVAER
                     TTELQAANHRLSMSDRRLTAMFEMSQRVHELDERQLLQLGVEEAVRHTDSAIGYLHLV
                     NEDQQTIELYTWSQGTLKYCSVAFDKHYPVAAAGIWADTVRTRSLVIHNDYQAMPGKK
                     GYPEGHAHVVRHLGVPVIEQGKVRLLLGVGNKITDYDASDADELQLIGNDLWSIVMRR
                     RAEVALSEAKDAAETANRAKSSFLANMSHEIRTPMNAIIGLSHLLSRSDLTPKQREQI
                     TKLGGSAQHLLGIINDILDFSKIEAGRMTVENAEFELDQVLRNVEGQLADRAEAKGLE
                     LVTDIDPNIPAMLIGDALRIGQILLNFGSNAIKFTEHGHLLLRVRTEAPNGGVARVRF
                     ELRDTGIGISTEQQTRLFAAFEQADASTTRRFGGTGLGLVISRQLARLMGGEVGVESA
                     PGKGSTFWLTLPLVLSKTVTAGKARLVQTALQGRRALVVDDLDEARNLLVSLLQSLGV
                     QAQSVASGQAAIEAVTQADSSQAPYDVLLIDWQMPELDGIETAAKVDQLGLQKPPTKI
                     LVTAFGHGLPSDVVRPGGFDGLLPKPVHASGLFDLLAGTLCNTGAKPAAAVRHDDGRA
                     LPKRRQVRILLVEDNRLNQEVTLDLLAEVGLNADIAENGEIAVALARQINYDLILMDV
                     QMPVMDGMEATRRIRLLPQHASTAILAMTANAFDEDRQACLAAGMNDHVAKPVDPERL
                     FAALEKWLPQSAKAAPRAAPQMPLPNQSVELLRRLPGLNVEAGLQVLRGRTDRYLQLL
                     QMFVRNHSSNVKALGDWLAHGDRDAARGEAHAIKGIAGTLGAYLLQQCATDLELAIRD
                     HAAPEPTAQLICRLDQALADLAQAVAVAEAAAAPNANSSALDGSTPTLANLAQLDAQL
                     ANDELAAAETFRQVRSQILNLIGARLTAQMDRHIAMFDFQEALAILRKALSDLNRPG"
BASE COUNT         1275 a         2106 c         2068 g         1455 t
ORIGIN      
        1 ccctccatca ccggcttacc ggccagcggg ccgatgtcgc ccagccccag cacggcggta
       61 ccgttggtga tgacaccgac caggttgccc cggctggtgt attcgtgcgc cattgccgga
      121 ttggcctgaa tctccaggca ggggtaggca acacctggtg aataggccag cgacaggtca
      181 cgctggttgt tcagcggctt ggtggcagta acagaaatct tgccacgggt gggggtgcgg
      241 tggtactcgg ccgttgcgtc gcgcagggcc tgctcagagg gggaaagtgg gttactcaaa
      301 tcttctccag ggttgaattg aagggcgcta ctgtactcct gcgcgcctgc ctatctttgg
      361 acttgggtca agctcggtgc aaaagatcat gcaaactgcg tgccatcacc tgggtggcgc
      421 ggcgcaaatc ggccaggtcg agccgttcat cggcgcgttt ggcatgcgat tcgagcacgg
      481 ttcggggtcc ggcaccgtaa atcacgccgg gaatgccagc ctcgcaaaac agtcgcacat
      541 cggtgtacag cggcgtgcca agggcggcaa tgggttcgcc aaacacggct tggccatgtt
      601 tctggatggc atcaaccagc ggctggttgc cagccaaggg tttcatgctg tgggccagca
      661 agatgcgccg cacatcaatg cggatgccgg gctggccagc ggcagtggcg gcaatcagct
      721 cgcgcaacgt ggcttcgact tgcgccgcgt tctcttccgg aatcatgcgt cggtcgagct
      781 tgaagctgac ccgccccgga atcacattgg tgttggtgcc gccttcgatc agcccgacgt
      841 tcaggtaggg gtggttgatg ccctccacct ctgaggtgat ggactggtag agcgtgtttt
      901 gcgcatacaa cgcgttgaga attttcactg ccgcctgcag ggcatcaacg ccggattcgg
      961 gaatggcggc gtgcgccatc tggccgtgca ccgtcacttc gagttgcagg cagccgttgt
     1021 gggcggtgat gacctggtag ctgaaacccg ccgcaatcat caggtcaggc tgggtgagct
     1081 tgtgtttgag caaccagcca gggccaacct cgccgccaaa ctcctcgtca taggtcagca
     1141 gcaggtcaat ctgcccttgc cggggttttg caactgcctc cagcgcacga atggcaaacg
     1201 aataagttga aaagtcgcat ttgctgacgg cgctggcacg gccataaatc ttgccgtcct
     1261 cgatctcgcc accatagggg tcgtgggtcc agccttcgcc aggcggcacc acatcgccat
     1321 gggcgttcag tgcaatggtt ttcccgccgg gtccatagtg gcggcgcacg atcaggttgg
     1381 tgatgctttg caggccggcg gcctgcactt cagcggcggg gaccgggtgt ttttctgccg
     1441 gcagccccat gtcggccagc agatccgctg cgcgctcggc gtgcggcgca ttgttgcccg
     1501 gtggcgtgtc ggtgggcacg cggatgagtt gctgcaaaaa cgccacttgt tcgtcaaagt
     1561 gggcatcgat ccaggcgtcg agttgggcgt agggtgtcat cggtttgatt gtgggttggt
     1621 taagcgggca ccggaccagg cacttgacgg cgatggcccg cagaattggg gtctgagccc
     1681 aattcgatga cgcgcgggcg acgcagcaaa cgccaggcgg tcgaattggg ttccaacccc
     1741 gattctgcta caccggtgct gtctgcagcg cttcacccgc ctgtcatggc gggttgtcca
     1801 cgtttgaccg gccgcgccac agcgacaacg cctcgcgctt gagacggtcg atgttgccca
     1861 gcaccgcacc aacggcggcg ctgccacacg gtccggcgct ttgctgccgg tcagcaaggt
     1921 atttggtttt atcagtcgtc tagggctgca gcccttgtca atactgtgca acaagctatc
     1981 atttaaatag taatctcgac ttgactgaag gctctcatcg gcgctgcttt catgccgccc
     2041 cagcacattg ccaagcgcgt cgatctgcac cgagtcaaag ccgcagtgct gcttctggga
     2101 gtccgacaag tcagctcctg gggcgctggt gggtcgtaaa aagctctgat gacacaagtg
     2161 acaaaggcag accttttttg gaaatatgcc gacggctcgg gacttgatgc ggcctcaacc
     2221 cggccggttc aggtccgaca gcgccttgcg caaaatggcc agtgcttcct gaaaatcgaa
     2281 cattgcgatg tgtcggtcca tctgcgcagt caatctggca ccgatcaagt tcaagatctg
     2341 gcttcgcact tgccggaacg tttcagcggc ggccaattca tcattggcca attgcgcgtc
     2401 cagttgcgca aggttggcga gcgttggcgt gctcccgtcc agggcacttg agttggcgtt
     2461 tggtgcagcg gcggcttctg cgaccgctac cgcctgagcc agatcagcca gcgcctgatc
     2521 aagccgacaa atgagttgcg ccgttggctc tggtgccgca tggtcgcgaa tggccagttc
     2581 aaggtcggtc gcgcactgct gcaacaggta ggctccgagc gtgccagcga tccccttgat
     2641 ggcgtgcgct tcgccgcgcg cggcatcccg gtcgccatgg gccagccagt cgcccaaggc
     2701 tttcacgttg ctgctgtggt tgcggacaaa catctgcaac aactgcaggt agcggtccgt
     2761 gcggccgcgc agcacctgca gaccggcttc cacattcaac cccggcaagc gacgcaacag
     2821 ctccaccgat tgattgggca gcggcatctg gggcgctgcg cgaggcgcag ctttggcgct
     2881 ctgtggcagc catttttcca gcgccgcaaa aagtctttcc ggatcgactg gtttggccac
     2941 gtgatcgttc atgccagcgg ccaggcacgc ttgccggtcc tcatcaaagg cgttggcagt
     3001 catggccaga atcgccgtac tggcatgttg gggcaggagg cgaatgcggc gtgtggcttc
     3061 catgccatcc atcaccggca tctgcacgtc catcaaaatc aggtcgtagt tgatttgccg
     3121 cgccagtgcg acggcaattt cgccgttttc ggcaatatct gcattgaggc ctacttcagc
     3181 cagcaggtcc aaggtcactt cctgattcag gcggttgtcc tcgaccagca gaatgcgaac
     3241 ctggcggcgc ttgggcagcg ctctgccatc atcatgacgt accgcagcgg ccggtttcgc
     3301 cccggtgttg catagcgtgc ccgccagcag atcaaacagc cccgaggcat gaaccggttt
     3361 gggcagcagg ccgtcaaagc ctccaggccg caccacatcg gacggcaggc cgtgcccaaa
     3421 ggcggtcacc aggatttttg tcggtggttt ctgcagaccg agctggtcga ctttggcagc
     3481 ggtttcgatg ccatcgagtt caggcatttg ccagtcaatc aacaacacat cgtaaggtgc
     3541 ctgcgatgag tcggcttgtg tgaccgcctc gatcgccgcc tggcccgatg ccacactttg
     3601 cgcctgcacg cccaggcttt gcaacaggct gacgagcagg ttgcgcgctt catccaggtc
     3661 atcaaccacc agcgcccgcc gcccctgcag cgctgtctga accaggcgtg ccttgcctgc
     3721 tgtgacggtc ttgcttaaca ccagtggcag tgtcagccag aaggtactac ctttgccggg
     3781 ggcactttcc acgccaactt cgccacccat caggcgtgcc agttgtcgcg agatcaccag
     3841 acccagaccg gtgccgccga atcgacgcgt ggttgatgca tcggcctgct caaacgcggc
     3901 aaacagccgg gtctgctgct cagtgctgat gccaatgccg gtatcgcgca attcaaagcg
     3961 cacccgcgcc acgccgccgt tgggcgcctc ggtgcgcacc cgcagcaaca ggtggccatg
     4021 ttccgtgaac ttgatggcat tgctgccaaa attcagcaaa atctggccaa tccgcaaggc
     4081 atcaccaatc agcatggcgg gtatgttcgg atcaatgtcg gtcaccagct ccagcccttt
     4141 ggcttcggcg cgatcagcca gttggccttc tacgttacgc agcacctgat ccagttcgaa
     4201 ctcggcgttt tcaaccgtca tgcgacccgc ctcgattttt gaaaaatcga gaatgtcgtt
     4261 gatgatgccc agcaagtgct gcgccgagcc gcccagtttg gtgatctgct cacgttgttt
     4321 gggtgtcaga tcgctgcgcg acagcagatg cgacagaccg atgatggcat tcatcggggt
     4381 gcggatttcg tggctcatgt tggccagaaa gctgctcttg gcacggttgg ctgtctcggc
     4441 agcgtccttg gcttctgaca gtgccacctc ggcgcgacga cgcatcacaa tggaccaaag
     4501 gtcgttgcca atcaattgca gttcgtcggc atcagacgca tcgtagtccg ttatcttgtt
     4561 gccaacaccc agcaataaac gaaccttgcc ctgctcgatc accggcaccc ccaggtgacg
     4621 gaccacgtgg gcatggcctt ccgggtagcc ctttttccct ggcatggcct ggtaatcgtt
     4681 atgaatcacc aggctgcggg tgcgcaccgt atcggcccag atgccagccg ccgccaccgg
     4741 gtaatgcttg tcaaaggcca ccgagcagta tttcaaggtg ccttgcgacc aggtgtagag
     4801 ctcaatggtc tgctggtctt cgttgaccaa atgcaggtag ccgatggcgc tgtcggtgtg
     4861 ccggaccgct tcttcgacac caagttgcag caattgccgc tcgtcaagtt catgaacccg
     4921 ctggctcatc tcaaacatgg ccgtcagacg ccggtcgctc atgctcaggc ggtggttggc
     4981 cgcctgcagc tcggtggttc gctcggcaac caggtgctcc agtttgtcac ggtgcaaaat
     5041 caactcgagt tccatctgtt tgcgttcggt gatatcttgc accgtgccaa gcgcaaacac
     5101 cggtgcaccg ctggcatcac gtcgaatctc ggcacgttcg cggacccagc ggatgtcggc
     5161 ctcgggcagt tgggccgaag cgccgatgac aatccggtgc tcaacatcga agtcaccgcc
     5221 ctgctgcgct gtcagccact cgcggcgcat ccaatccaga tcgttgggat gaacgctagc
     5281 aacgaacttt ctcaagctga tgggttcatg gcggctcagg ccaaatatgc ggtaggcctc
     5341 gtcagacccc tgcagttcgc cagtctgaac cgtcaggctc caactgccga cgcgggccac
     5401 cgcctgcgct tcacgcaggt cacgttcgct gtttttcaac tgttcctcgg ccagcttgcg
     5461 acctgtcatg tcggtaaatg aggtcaccac gccagcggga ccttgcccgc taccgccagg
     5521 caacacctgg gcattgatcg aaatccagac catctggccg ttgtcgccgg gcagacccag
     5581 gaccacatcg cgttgtgcct ggccactttg aagcgcgcgg ctggtcggca gttcctcggc
     5641 ggggaagctg ctgccgtcct cgcgtaccgc gcgccaactg gcatcgacac cactgcgtcc
     5701 cagcaaatca agctcggcaa cgcccaaaat acgctctgct gccgggttac aggcgcgaat
     5761 gattccccca ccatcgagca cgatgatgcc ctcgttcatt gaggtgacga ccgagtggta
     5821 aagcgcctgt ctgacggcaa tctcttcacg gatgcgcttt tcttcggtga tgtcagacca
     5881 ggtgctggcc aggtactcgc gcccagccac ctcgatcctg ctgacgctga cgcgcacatc
     5941 acgcaggctg ccatctttgt ggcgatgcct tgactcgaac acggcagatc cgctggtggc
     6001 aatccgtgcg aagctttgct gcacctgggc tgggttctgg tcggcctcga tgctggccac
     6061 acccaatgcc gcgaactctt cacgcgagta acccagcctg gccgctgcag catcgttgaa
     6121 ttcgacaaat ctgccggtgt cgaaatccac caagcccatt gaatcggcgg cgcggcgggt
     6181 cattgccgca aaaattgcct cgcgctcgcg caaggtctgg cgcacctggc gttcattcat
     6241 tcggccagcg atacgcgcgg cgatggtctc cagcagcgtc tgctcttgtg acaaaaagac
     6301 cagatggtgg ccagtccggg gttcatcgcg gtaggcgatt tcaatggaac ctacttcaac
     6361 accatcaatc ggcaggggcg cgcgctgact cgggtgacct gcctggtagt tggcaccggc
     6421 aatgtgccga tgcggcagcg tgatgcgggc cgcgacctgt tcggggaatt gccagccggc
     6481 cggtagccgc tcgaccacag cctgcagttg cgtgtcgaaa tcgccgtggt cgtcttgggt
     6541 cagggtaaaa atgtcgtaca ggcaacgctg ttccttgatg cgttcttcaa gctcatgttg
     6601 cgtggctcgt tgctgagtga tttcgtgcga aacgcccagc acaccgatga cccgaccggt
     6661 ggcctcgacc agcggcatct tggtggtctc atacaagcca gaatgaccac caacggccat
     6721 ggtcagccat tcttcgttgc aggtgggttg tccagccgat gcggccattc ggtcatttgc
     6781 ccggaaaaag tctgcctgcg ccggatcaac aaaatcgtag tcggtcttac cgacgatctg
     6841 gtcttccttt gtggccgcca gtttgaacat gctgaatttc agccggtttg aaaatgtttg
     6901 gcga
//
LOCUS       DAOSLL010000189         6860 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10041_length_6860_cov_4.865393, whole genome shotgun sequence.
ACCESSION   DAOSLL010000189 DAOSLL010000000
VERSION     DAOSLL010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6860)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10041_length_6860_cov_4.865393"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="PLB25_09715"
     CDS             <1..781
                     /locus_tag="PLB25_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOE41890.1"
                     /translation="SVALTWLVCGLVMVGYSVASSLNSRMGWNDAAFGALVAAFPNSG
                     FMGVPLLVALLGTTAAGPTILTIAIDMIVISSLCIGLSRLDGADQHGAARAFRNALQG
                     VLANPMPWSILLGALVSVLGFELPQPVAQVVGLLADAASPVALFTIGAVLARSQMLAA
                     AGHTVAPLLRDYLPLVLQKLFMHPLLVLLLGASLIRLGLPLSRFALTVLVLVAALPSA
                     SNVSLLAERYGANNGRVARIILVSTAAAFVTFSLSVALLLP"
     gene            complement(942..3116)
                     /gene="priA"
                     /locus_tag="PLB25_09720"
     CDS             complement(942..3116)
                     /gene="priA"
                     /locus_tag="PLB25_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019658301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HOE41891.1"
                     /translation="MHTPAYSQLAGALTYLCDKALPPGTLVRVPLGQRELLGVVWDVA
                     PGQPESGAAPMPDPAKLRPIAAALLALPALSASWRALIGFAASYYQRSPGEVALAALP
                     AQLRDLSTLQLARRLSRPGAKKAGVKPENPAGPDHSRPLAEHAGQMGGLPGDPQDAIE
                     SVALSAEQTRAIEQFHASPGPFLLFGATGSGKTEVFMRCTADLLAGAADAQALVMVPE
                     INLTPQLEQRFKARFCPLYGDDAVVSLHSGMTPAQRLKSWLAAHSGAARIVLGTRMAV
                     FASMPGLRLIIVDEEHDPSYKSHEGARYSARDLAIYRARLDAAKVMLGSATPSLESWH
                     HSRPASEGGRYLRIDMPSRVGANALLPLVRRVDMNHQPKACAISPPLLDAMAQRIGRG
                     EQSMVFLNRRGYAPVLSCGDCDWKSTCPNCSAYRVFHKIDRSLRCHHCGYTEPVPRAC
                     PDCGNPDIAPMGRGTERLEEHLGHLLCSVSRPDGQAVRIVRIDADSTRLKGALQSQLA
                     QVHSGEVDVLVGTQMIAKGHDFRRITLVAAINPDGALFSSDFRAPERLFSLLMQAAGR
                     AGRDASNTSRSEMWLQTFHPTHPLFEALKRHDYPAFAQSQLLERQQAGMPPFSFQALV
                     RAEARTQEAAQGFLNAASAAASELAAVAHLLAHITLYPAVPMSIQRVANVERAQMLIE
                     SPSRTALQRFLTAWQATLHATRGRPENKGLLRWAIDVDPLLI"
     gene            complement(3501..4604)
                     /gene="hemE"
                     /locus_tag="PLB25_09725"
     CDS             complement(3501..4604)
                     /gene="hemE"
                     /locus_tag="PLB25_09725"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HOE41892.1"
                     /translation="MNTSATSPYAPLKNDTFLRACLRQATDYTPVWMMRQAGRYLPEY
                     RATRARAGSFMGLATNTDYATEVTLQPLERFPIDAAILFSDILTVPDAMGLGLSFALG
                     EGPRFANPVRDEAAVAMLQVPDMDKLRYVFDAVTSIRKALNGRVPLIGFSGSPWTLAC
                     YMVEGAGSDDYRLVKTMLYSRPDLMHRMLQINADAVALYLNAQIEAGAQAVMIFDSWG
                     GVLADGAFQDFSLAYTRQVLSKLHKQYNGARVPSIVFTKGGGIWLREMAELDCNVLGL
                     DWTVNLAKARALVGGSKALQGNLDPNVLFAKPAQIETEVRKVLTAFGTPHTGFGDGPT
                     HIFNLGHGISQHTPPAHVEALVQAVHRHSREMR"
     gene            complement(5623..6471)
                     /gene="purU"
                     /locus_tag="PLB25_09730"
     CDS             complement(5623..6471)
                     /gene="purU"
                     /locus_tag="PLB25_09730"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HOE41893.1"
                     /translation="MNPVYTLTLSCPDRLGLVHAVSGFLLERSGNIEEAAQYNDSGTG
                     LFFMRVQFACAKLSFDELKAQLASFAQPFQMKWQLHAQAQPMRTVILVSKEGHCLNDL
                     LFRWRSGLLNLDIGAIISNHRDFYQLAASYNVPFHHLPVTAATKAQVEARQLEIIQAE
                     QAELVVLARYMQILTSALCQELSGRAINIHHSFLPSFKGAKPYYQAHDRGVKLIGATA
                     HYVTENLDEGPIIEQDVARVDHSKTVEDLTALGRDTESQVLARAVKWHSEHRVLINGH
                     KTVIFK"
     gene            complement(6584..6859)
                     /locus_tag="PLB25_09735"
     CDS             complement(6584..6859)
                     /locus_tag="PLB25_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3567 domain-containing protein"
                     /protein_id="HOE41894.1"
                     /translation="MTHMLANAVAADVGPDTLKDGEQLLIVPRLARHGFEIVDKRSNK
                     EVYLDGSWAELFQQQITAWQQKTPTQEEVEDTLEQYAELAQNPVVMH"
BASE COUNT         1253 a         2081 c         2129 g         1397 t
ORIGIN      
        1 ctcggtggcg ttaacctggc tggtatgcgg cctggtgatg gtcggctaca gcgtggccag
       61 cagcctgaac agccgcatgg gctggaacga tgcggctttt ggcgcgctgg tggcggcctt
      121 tcccaactcg ggtttcatgg gcgtgccact gctggttgcg ctgctgggca caacggcagc
      181 cgggccgacc atcctgacca tcgcgatcga catgatcgtc atcagttcgc tgtgcattgg
      241 gctgtcccgc ctggacgggg cggatcagca tggtgctgcc cgtgccttca ggaatgccct
      301 gcaaggggtg ctggccaacc cgatgccctg gtcgatcttg ctcggcgcgc tggtgtcagt
      361 gctggggttt gagctgcccc aaccggtcgc ccaggtggtc gggctgctgg ccgatgcggc
      421 atcaccggtg gcgctgttca ccattggtgc ggtattggcg cgttcgcaaa tgctggccgc
      481 agccggccat accgtggcac cgctgctgcg cgactatctg ccgctggtgc tgcagaagct
      541 gtttatgcac ccgctgctgg tgctgctgct tggcgccagc ctgattcgcc tgggcttgcc
      601 cctgagccgc tttgccctga cggtgctggt gctggtggcg gccctgccca gcgccagcaa
      661 tgtgtcgctg ctggccgagc gctatggtgc caacaatggc cgggtggcgc gcatcattct
      721 ggtgtccacc gccgcagctt ttgtcacctt ctcgctatcg gtcgccctgc tgctgccgta
      781 agccggacaa gctggaatcg aacagtattc gtcgcgaggg cgcgactccc acagaacatc
      841 tgacacattc tgaagatgtg ggagttgcgc cctcgcgacg aatgtcgcca tgacttatct
      901 gaattcttcc cggaaatcgc aaaaacttga cttgtcagtg actagatcag cagcgggtcc
      961 acatcaatcg cccagcgcag caggcccttg ttttccggtc ggccccgggt ggcgtgcagc
     1021 gtggcctgcc aggctgtcag aaatcgctgc aaggcggtgc gtgacgggct ctcgatcagc
     1081 atctgggcgc gctccacgtt ggcaacgcgc tgaatgctca tcggcaccgc cgggtacagc
     1141 gtgatgtgcg ccagcaggtg tgcgaccgct gccagctcgc tggctgctgc gctggcagcg
     1201 ttcaaaaacc cctgggcggc ctcttgcgtg cgggcctctg cgcgcaccag cgcctgaaag
     1261 ctgaacggtg gcatgccggc ctgctggcgc tcaagcagtt gcgactgcgc aaacgccggg
     1321 tagtcgtggc gcttgagtgc ctcaaacagc gggtgcgtcg ggtgaaaggt ctgcagccac
     1381 atttcgctgc ggctggtgtt gctggcatcg cgcccggccc gccctgcggc ctgcatcaac
     1441 aggctaaaaa gccgctcggg ggcgcgaaag tcgctggaaa acaaggcccc gtccgggttg
     1501 atggcggcca ccagcgtgat gcggcgaaag tcgtgcccct tggcgatcat ctgcgtgcca
     1561 accagcacat caacctcgcc tgaatgcacc tgcgccagtt gcgattgcag cgcgcccttg
     1621 agccgggtgc tgtcggcatc aatgcgaaca atccgcacgg cctggccgtc cggacggctg
     1681 acgctgcaca gcaggtggcc gaggtgttct tcgagccgtt cggtgccacg gcccatcggt
     1741 gcgatgtcag ggttgccgca gtccgggcag gcacgcggca ccggctcggt gtagccgcag
     1801 tggtggcagc gcaagctgcg gtcgatcttg tgaaacaccc ggtaggcgct gcagttcggg
     1861 caggtgcttt tccagtcgca gtcgccgcag ctcaacactg gcgcataacc acgccggttc
     1921 aaaaacacca tgctttgctc accccggcca atgcgttgcg ccatcgcatc gagcagcggt
     1981 ggcgaaatcg cgcaagcctt gggctggtgg ttcatgtcaa cccggcgcac cagcggcagc
     2041 agcgcgttgg caccaacccg gcttggcatg tcgatgcgca ggtagcgccc gccttcgctg
     2101 gcagggcggc tatggtgcca gctctccagc gacggcgtgg ccgagcccag catcaccttg
     2161 gcggcatcaa ggcgggcgcg gtaaatggcc agatcgcgcg ccgaataacg cgcgccctcg
     2221 tggcttttgt agcttgggtc gtgttcttcg tcaacgatga tcaggcgcaa acccggcatc
     2281 gaggcaaaca ccgccatgcg ggtgcccagc acaatccgcg ccgcgccgct gtgcgccgcc
     2341 agccagcttt tcaggcgctg cgccggggtc atgccgctgt gcaaggacac caccgcgtca
     2401 tcaccgtaaa gcgggcaaaa gcgcgccttg aagcgctgct caagctgcgg cgtcaggttg
     2461 atttcgggca ccatcaccag cgcttgagcg tcggcggcac cggccagcag gtcagccgtg
     2521 cagcgcatga acacctcggt cttgccgctg ccggtggcac caaacagcaa aaacggccca
     2581 ggactagcat gaaattgctc gatagccctt gtctgctctg cgctgagggc tactgattca
     2641 atagcgtctt gcgggtcgcc tggcagcccg cccatttgcc cggcatgctc agccaggggt
     2701 cttgagtggt ccgggccagc cgggttttct ggcttgactc cagccttttt ggcccctggg
     2761 cggctcaacc ggcgcgccag ttgcagggtg ctcaggtcac gcagttgcgc cggcaaggcc
     2821 gccagcgcca cttcacccgg cgagcgctga tagtagcttg ccgcaaaccc gatcagcgca
     2881 cgccagcttg ccgagagcgc tggcagggcc agcagcgcgg cagctatcgg gcgcagtttg
     2941 gccgggtcag gcatgggcgc agcgccagac tctggctggc cgggcgcgac atcccacacc
     3001 acgccgagca gttcgcgctg ccccagcggc acccgcacca atgtgcccgg cggcagggcc
     3061 ttgtcgcaca ggtaggtcag cgccccggcc aattggctgt acgccggggt gtgcagcagc
     3121 accgacacct gctgcggcgt ggattgctct ggttcggctg ggttgttcaa gtgaacttgc
     3181 gcaatatcgc cctaagtggt tgattctttg tagctttaca agtcatccaa gttgtctgtg
     3241 gataactttg tgcatagatc gtcggctgag ccgcccaagc cttgcaaatc aaggcttccg
     3301 acggtttgcc taaaaaacat gcaaaaacaa taaaccattt taaatcaacg agttacactc
     3361 gctatgcgtt tgctagcagg gggcactact gttttcatag ttgaccccgg aaaatcccta
     3421 tttttgtgca taagtaatta ctttttcgtc atcaggcacg ccggaatggg aaaaaaagtg
     3481 ctagcgcagc cagttctggc tcaacgcatt tcgcgcgagt ggcggtgcac cgcttgcacc
     3541 agcgcctcca catgcgctgg cggggtatgc tgactgatgc catggcccaa gttaaaaata
     3601 tgcgtcgggc cgtcgccaaa accggtgtgg ggggtgccaa atgccgtcag caccttgcgc
     3661 acctcggtct cgatttgcgc cggtttggca aacagcacat tcgggtccag gttgccctgc
     3721 agcgccttgc tgccgcccac cagtgcgcgg gccttggcca ggttcacggt ccagtccagc
     3781 cccagcacat tgcagtcaag ctcggccatt tcacgtagcc agatcccgcc acccttggtg
     3841 aacacaatgc tgggaacgcg cgcgccgttg tactgtttgt gcagttttga caacacctgg
     3901 cgggtatagg ccaggctgaa atcctgaaag gcaccgtcgg ccagcacgcc gccccaactg
     3961 tcaaaaatca tcactgcctg ggcaccggcc tcaatttgtg cattcaggta caacgccacc
     4021 gcgtcggcgt tgatctgcag catccggtgc atcaggtctg gccgactgta gagcatggtt
     4081 ttgaccagcc ggtagtcgtc tgaaccggca ccttccacca tataacaggc cagtgtccac
     4141 gggctgccag aaaaaccgat cagcggcacc cggccattga gcgccttgcg aatcgaagtg
     4201 accgcgtcaa acacatagcg cagcttgtcc atgtcgggca cttgcagcat ggcaaccgcc
     4261 gcctcatcgc gcaccgggtt ggcaaaccgg gggccttcgc cgagcgcaaa gctcaagccc
     4321 agtcccatgg cgtcgggcac cgtcaggatg tcgctgaaca aaatggccgc gtcaatcgga
     4381 aaacgctcca gcggctgcag agtgacttcg gtggcgtagt cggtattggt ggccagcccc
     4441 atgaagctgc cagcccgggc acgggtggca cggtactctg gcaaatagcg cccggcctgg
     4501 cgcatcatcc agaccggcgt gtagtcggtc gcctggcgca ggcaggcgcg cagaaaagtg
     4561 tcattcttca ggggagcgta gggcgatgtg gcagaagtat tcattgctga attgtcgcat
     4621 tcgcatgccc tggcgatttg acttcaccag caagcatgac gcatgcggcc atttccggtc
     4681 gtggcggcat gaacccggat tgccagcttt gtctggcaac acccccggca tcatgtcggc
     4741 taaaacaccg ttgtcgccag ccgggacatg accatcgagg tagtgggact gtcgcctggc
     4801 ttgaaattgg aatgcgacct ggtcctgaca cgccgggcct gcttgcattg ggtggatggt
     4861 cagcctcttt gcagtctttt gttttcttgt ccgacgacca cgactcatct gaaccaagct
     4921 tcacctgcat ctgagcgcgc tccaggaatt gccagggcgc tgactcatgc cgcatcggct
     4981 gctgcccgcg tagctgctgt gtatgcctgt ggtggcgctg ctgccgcccc tgacacctgc
     5041 tgccacgccg cctgaaccgg cagttacggt tgtagccgca gccatcggca ggccgtcaag
     5101 ccacgactgt gaaacaaaat gtcacacccg acaaggccaa taggctggcc acagatttac
     5161 tctgggggtg tgctgttcac agtgagcatt gccgcagtcc ggcatgagga tgtggtcaga
     5221 aatgacttcc cctatttgca ccggcggatt cgggcgcaca gcgtcgttgc aagacgcttg
     5281 tttagccagc ggtatctgcg gctgcctggc ctgcagaggt aaacaataca gcggagagtc
     5341 cccagcgatg attcttgatg ttggcagtgg ctggaagaac tggcactcgg aacgtgtcgt
     5401 gatcgaaggc gaaagatggt agctcctcaa taagtcgcgg caaaacccgt cattcccgtc
     5461 tgcgcgggaa tgacggaatc gtttcatttt gcgagtttcg ccacgggtgc gtgcgcaggc
     5521 atgtttggag ccgcagcttt cggcgtagcc agacgcgccg ccagcggccc actcgggcgc
     5581 ggtcaactga gcaatccagg acgacctgat tggcgtgagc ggctacttga aaatcaccgt
     5641 cttgtgcccg ttgatcagca cccggtgttc gctgtgccac ttgaccgcac gggccagcac
     5701 ctggctttcg gtgtcgcggc ctagcgcggt taagtcttct accgttttgc tgtggtcaac
     5761 ccgggccaca tcctgctcga tgatcgggcc ttcgtccagg ttttctgtga catagtgggc
     5821 ggtggcacca atcagcttga caccccggtc atgtgcctgg tagtaaggct tggcaccctt
     5881 gaagctgggc aagaacgagt ggtggatgtt gatggcgcgg ccgctgagtt cctggcacag
     5941 ggcgctggtc aagatttgca tatagcgcgc cagcaccacc agttcggcct gctcggcctg
     6001 aatgatctcc aactgcctgg cttcgacctg cgccttggtg gcggcggtca cgggcaggtg
     6061 gtgaaacggc acgttgtagc tggcggcaag ctggtaaaaa tcgcggtggt tggaaatgat
     6121 ggcaccaatg tccagattga gcaggccgct tctccagcga aacagcaggt cgttcaaaca
     6181 atggccttcc ttgctgacca ggatgacggt gcgcattggc tgcgcctggg cgtgtaattg
     6241 ccatttcatc tggaacggct gggcaaaact ggccaattgg gctttgagtt cgtcaaaact
     6301 gagtttggcg caggcaaact gcacccgcat gaaaaacagg ccggtgccgg agtcgttgta
     6361 ttgggcggct tcttcgatat tgccactgcg ttcgagcaaa aagccggaaa ccgcgtggac
     6421 cagcccgaga cggtcagggc aggacagggt caaggtgtaa acaggattca taggggcggt
     6481 attgtcgcag ttgccgccag accagaaagg ttggctggtt agctacataa attatagcta
     6541 ccaaggcact atccataagg gctagtgccc tattttatgc caactaatgc atcaccaccg
     6601 ggttttgcgc cagctcggcg tattgctcca gcgtgtcctc gacctcttcc tgggttgggg
     6661 ttttttgctg ccaggcggtg atctgttgct gaaacaactc ggcccaggag ccgtccagat
     6721 agacctcttt gttcgagcgt ttgtcaacaa tttcaaagcc gtggcgtgcc agtcggggca
     6781 cgatcagcag ttgctcgccg tccttgagcg tgtccggacc cacatctgcc gccaccgcgt
     6841 tggccagcat atgggtcaca
//
LOCUS       DAOSLL010000190         6857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10051_length_6857_cov_3.884887, whole genome shotgun sequence.
ACCESSION   DAOSLL010000190 DAOSLL010000000
VERSION     DAOSLL010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6857
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10051_length_6857_cov_3.884887"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..124)
                     /locus_tag="PLB25_09740"
     CDS             complement(<1..124)
                     /locus_tag="PLB25_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020485772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HOE41895.1"
                     /translation="MLDDIKKTLWATADKLRANMDAAEYKHLVLGLIFVKYISDT"
     gene            complement(463..1041)
                     /locus_tag="PLB25_09745"
     CDS             complement(463..1041)
                     /locus_tag="PLB25_09745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HOE41896.1"
                     /translation="MPQLLSVQTGTVRPVVMQGRKVLTAIRKQAVVGPVPVWPLGLQG
                     DEQADPAVHGGLDKAVYAYPSEHYPRWQAARLQAGLADIDNGLPWGSMGENLSLQGLL
                     EADVWVGDVLRFANCSLRVTRPREPCYKFNAAMGLPTAAKTMALSGCCGFYLAVEQVG
                     SLQAGESFELVRGQRGLSIAEAFKAKMFKHLR"
     gene            1188..2138
                     /locus_tag="PLB25_09750"
     CDS             1188..2138
                     /locus_tag="PLB25_09750"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE41897.1"
                     /translation="MLAQLLRGLYLFQMLLGGLLGAWLAAPLTHQDGSALAWLLVPLG
                     ALGLPLLLQFLTMIALVLRARDGGPLSDWWRAFWGEYFAAIAIFMLRQPWPRRASEVL
                     LPLPGAPNPIGAAPMPVLLVHGFICNHRVWDDVAAALRRAGHPVLAIDLEPLFTSIDD
                     YAEPIELAADKLLACTGARQLALVGHSMGGLAIRAWLRAHGSGRVAKIITLGSPHQGT
                     HIVKALITANGEQMLWRSAWLQALQDSEKPATRHLMQIALSRQDNVVYPQHEQVLSDA
                     SVTEFSGLGHLQLCLNPAVINWLVQQLASHQSDPATGKPV"
     gene            2165..2773
                     /locus_tag="PLB25_09755"
     CDS             2165..2773
                     /locus_tag="PLB25_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOE41898.1"
                     /translation="MSVVKPPPADIAPPGPRSLADLFWAFTWLALQGFGGVLAVAQSE
                     MVDKKRWLTREQFLEDWAVAQVLPGPNVVNLSLMIGDRYFGVRGALAAMAGMLAFPVV
                     ILLLLAILFASVADLPQVQGTLRGMSAVAAGLIAATGLKLVPSLKGNVLGALVCWALV
                     AITFISIGWLRVPLAWVLPGLGGTASLWAYRQLGRDTAKGDC"
     gene            2773..3369
                     /locus_tag="PLB25_09760"
     CDS             2773..3369
                     /locus_tag="PLB25_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HOE41899.1"
                     /translation="MSITLDASDWLALLGHFMSLSLLAIGGVIATAPDMHRTLVDQNH
                     WLYDSQFNTCVALAQAAPGPNVLFVALLGWQVGLNAGGGIAGGFVAWASAALGMVLAM
                     LGILLPSTTLTLAASRWAHQNRELRAVRAFKQGMAPVVVALLIATSWILAVNLGTSAG
                     HWPLWLLVVVSTLLVWRTRLHLLWLLAGGGLLGWFGLV"
     gene            3402..4187
                     /gene="modA"
                     /locus_tag="PLB25_09765"
     CDS             3402..4187
                     /gene="modA"
                     /locus_tag="PLB25_09765"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE41900.1"
                     /translation="MFKKLLGAIAASLLLLTAPVQAQKAEPEMLLYCGITMVKPMTEI
                     ARLFEKREGLKILISQGGSEDLYQSAKKSGIGDWYLPGEPSYRDKHLKEGLFGDFVNV
                     GYNQMALVVQKGNPKKVKADPFEMLRKDLTVILGNAESGSVGQESKTVLDRLGIYPKV
                     VKASAFLSPDSRSLMNSMKKGEADLTMTWRATGLFADNIAKLDVIDMDPKIAKPQALL
                     LIMLKSTKNAEMARSFMKFVASEEGQAIYRKHGFLDNKAVVTR"
     gene            4216..>6857
                     /locus_tag="PLB25_09770"
     CDS             4216..>6857
                     /locus_tag="PLB25_09770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE41901.1"
                     /translation="MKTTSDAAQSGHAAAASVDSSLRWLVGVSLVCLVLVFGLRSYFS
                     DLENEIKQRGDNERARLFVGEEIVRGIQEIEKDIYHMAVTQNSAGLARINAEIDGRLV
                     KLEHDLQVLQNGGISRRQLQLNIDGTEEVTREASYRPDAANQNVVMELIEIEPQLGQI
                     RARAAMLGQLLERRWKSLEIEDKKAFLSAEEEVVIELKQIPPQFERLNENANRLFLEG
                     DQRLRALEAELQRRTSRLKQIETGLLLMVVVLGGVLAVLFMRRLTGALRETRQARDDI
                     ELQREQSANILDTLADGVYSTDMKGLITYVNAAGERILGWSARELIGQQAHQAIHHTR
                     PDGTPFSPDTCPLIAVVEKGETLDGEDHFVSRDGQFVPVSYRSKPLLQAGRVVGSLLS
                     FHDISERLEREARIRLQQAALDAAVNMIVITSRNGLIEYVNPAFCRTTGFDANEVLGH
                     SPGILKSGQHEAGFYKNMWDCLLSGKAWEGELNNQRKNGEIYPEQMTITPIFENREIA
                     HFIAIKRDISEEIRTRTRLKLIESAIQETDQGIHIMDATPHALGPVIQYVNAGFSRIT
                     GYGADEAVGARAGFMQSPTTDPGKLGLISQAMLQGSSLTLEMNYQRKDGSRYVGELHL
                     SPVHADSGKVSHYIGLLSDIDLRKQAELALREARDQALENSRLKSEFLSTMSHEIRTP
                     MNGIIGMTDLLLDTELDPEQRDFTGIVRDSAQALLVIINDILDFSKIEAGKLEIDITE
                     FSCSHVVEGTLELLTSRAHEKKLTLTSFVDPALPARLMGDPTRLRQVLLNMIGNGIKF
                     TESGGVEVNALLVNTGSTAMVRFEITDTGIGISQQTQAKLFQSFTQADSSTTRKYGGT
                     GLGLAICTRLVELMGGQVGIESTPG"
BASE COUNT         1340 a         1990 c         2118 g         1409 t
ORIGIN      
        1 aggtgtcgct gatgtatttg acgaagatca ggcccagcac caggtgcttg tattcagcag
       61 cgtccatatt ggcgcgcagc ttgtcggcgg tggcccagag ggtcttcttg atgtcgtcga
      121 gcatggggtg ttgtcgtccc tgtcagtctg ttttgtaccg ccgcagatga tgcaacctga
      181 ccagctctgg ggcagggctg gaggcgcatc aacggccagg ttgaccacgc actgggtttg
      241 gtccacagaa cttcggtagc catagccggt tcgtgccagc cagatgtgcc ggttgaagga
      301 acttcttgcc cgtgccggta tgcccagcat tgagctaagc ggattgccca acgccctgcg
      361 ccatgacagc cgagcctcgc ttgcggccgg gccatttcat gcaaacccct tcccatcgct
      421 acctgattgc atcaacaggc tgcgtcatgg ccgccatgca gcctagcgca gatgcttgaa
      481 catcttggcc ttgaatgcct cagcaatgct caggccgcgc tggccacgga ccaattcaaa
      541 cgactcaccg gcttgcaggc tgccgacctg ctccaccgcc aggtaaaagc cgcagcagcc
      601 cgacagcgcc atggtttttg ccgccgtcgg caagcccatc gccgcattga acttgtaaca
      661 gggttcgcgc ggccgggtca cgcgcaggct gcaattggca aaacgcagca catcaccaac
      721 ccacacgtcg gcctccagca ggccttgcag gctgaggttt tcgcccatgc tgccccacgg
      781 caggccgttg tcgatgtcag ccagcccggc ttgcaggcgc gccgcctgcc accgcgggta
      841 gtgctctgac gggtaggcat aaaccgcctt gtccaggcca ccgtgcaccg ccgggtcggc
      901 ctgctcatcg ccttgcaagc ccagcggcca aaccggtacc gggccgacca cagcctgctt
      961 gcggatggcg gtcagcacct tgcgaccctg catcacaaca ggccgcacgg tgccggtctg
     1021 aacgctcaga agttgtggca tgtgttgtcc tttgaaaccg cgcagtctcg cacgtccgac
     1081 cagcccgggt agatcaaaaa tgccgcgacc ggatcagcgc agcgctggcc agggcagcgg
     1141 ggcttgaccg acaatcgggg ttctgcaatt ttcctgatgg tttggccatg cttgcccaat
     1201 tgcttcgcgg tttgtacctg tttcaaatgc tcctgggtgg tttgctcggt gcctggctgg
     1261 cagcgccgct gacacaccag gatggtagtg cgctggcctg gctgctggta ccgctgggcg
     1321 ctctgggcct gcccttgctg ctgcaatttt tgacaatgat tgccctggtg ctacgcgcgc
     1381 gcgacggcgg gccgttgtct gactggtggc gcgcattctg gggtgagtac tttgccgcaa
     1441 ttgccatttt catgctgcgc caaccctggc cccgacgagc gagcgaggtt ttgctgccac
     1501 tgccgggcgc acccaaccca atcggtgctg cgccaatgcc agtgctgctg gtgcacgggt
     1561 tcatctgcaa ccaccgggtt tgggacgatg tggcggctgc cctgcgccgg gctggccacc
     1621 cggtgctggc gattgatctg gagccgctgt tcacctcgat tgacgactac gccgaaccca
     1681 tagagttggc ggcagacaag ctgctggcgt gcactggtgc caggcagttg gcgctggttg
     1741 gccatagcat gggcgggctg gcgattcgcg cctggctgcg cgcacatggt agcggccggg
     1801 tggcaaagat catcaccttg ggcagcccgc atcagggcac ccacattgtc aaagccctga
     1861 tcaccgccaa cggcgagcaa atgctctggc gcagcgcctg gctgcaagcc ttgcaggaca
     1921 gcgaaaagcc cgctacccgg cacctgatgc agatcgccct gtcgcggcag gacaacgtcg
     1981 tgtatcccca gcacgagcag gtgctcagcg atgccagcgt caccgagttc agcggcctgg
     2041 gccatctgca gctttgtctg aatccggctg tgatcaactg gctggtgcag cagcttgcca
     2101 gccaccaaag tgacccggcg acgggcaagc cagtctgaat cacgcgcgcc gcgcgcatcg
     2161 cgccatgtcc gttgtcaagc caccccctgc cgacatcgcg ccgccaggcc ccaggtcgct
     2221 ggcggatttg ttctgggcct ttacctggct ggcgctgcaa ggctttggtg gtgtgctggc
     2281 ggtggcgcaa agcgagatgg tggacaaaaa gcgctggctc acccgtgaac agttccttga
     2341 ggattgggcg gtcgcccagg tattgcccgg acccaatgtg gtcaatttgt ccctgatgat
     2401 tggtgaccgt tactttggcg ttcgcggcgc tctggcggcc atggccggca tgctggcttt
     2461 tccggtggtc atcctgttgc tgttggcgat cttgtttgcc agcgttgctg acctgccaca
     2521 ggtgcagggc acgctgcgag gcatgagcgc cgtggcggcc ggtctgattg ccgccacggg
     2581 cctgaaactt gtcccttctt taaagggaaa tgtgcttgga gcgcttgtct gctgggcgct
     2641 ggtagctatt acttttatat caattggatg gcttcgggtg cctctggcct gggtgctgcc
     2701 ggggctcggt ggcaccgctt cgctttgggc ttaccggcag ttgggccggg acaccgccaa
     2761 aggcgactgt tgatgagcat cacgctggac gcatcggact ggctggcgct gctggggcat
     2821 ttcatgtcac tgtcgctgct ggcgattggt ggtgtcatcg ccaccgcgcc agacatgcat
     2881 cgaaccctgg tcgatcagaa ccactggttg tacgacagcc agttcaacac ctgtgtcgcg
     2941 ctggctcagg cggcacctgg tccaaatgtg ctgtttgtcg ctttgctggg ctggcaggtg
     3001 ggtttgaatg ccggcggcgg catcgcgggt gggttcgtgg cctgggccag cgctgccctg
     3061 ggcatggtgc tggccatgct gggtatcttg ttgcccagca cgacgctgac cttggccgcc
     3121 tcgcgctggg cgcatcaaaa ccgggagctg cgggccgtca gggctttcaa acaaggcatg
     3181 gcgccggtgg tggtggcgct gttgatcgcc accagttgga tactggcggt gaacctgggc
     3241 acgtcggccg ggcattggcc attgtggctg ctggtggtcg tgagcaccct gttggtctgg
     3301 cgcacccggc tgcatttgct gtggctgctg gccggaggtg gcctgttggg ctggtttgga
     3361 ttggtctaac attcggtcat gttcatttac aggtggtact gatgtttaaa aaattgttgg
     3421 gtgccattgc cgcatccctg ctgctgttga ctgcccccgt tcaggcgcaa aaggccgaac
     3481 ccgaaatgct gctgtactgt ggcatcacca tggtcaaacc gatgacggaa attgcccgtt
     3541 tgtttgaaaa gcgcgaaggg ctcaagattt tgatctctca gggtggatca gaagatttgt
     3601 accagtcagc caaaaaaagc ggcataggtg actggtattt gccgggcgag ccgtcttacc
     3661 gtgacaaaca cctgaaggaa ggcctgtttg gtgattttgt caatgtcggt tacaaccaga
     3721 tggcgctggt ggtgcaaaaa ggcaacccga aaaaggtcaa ggctgacccg tttgagatgc
     3781 tgcgcaagga tctgacggtg attttgggca acgccgagtc gggcagcgtg ggtcaggaat
     3841 ccaaaaccgt gctggaccgc ctgggcatct acccgaaggt ggtcaaggcc agtgcctttt
     3901 tgtcacccga ttcgcgcagc ctgatgaact ccatgaaaaa gggcgaggct gatctgacca
     3961 tgacctggcg cgccaccggg ctgtttgccg acaacattgc caaactcgac gtgatcgaca
     4021 tggaccccaa gattgccaag ccgcaagcac tgttgctgat catgctcaaa agcaccaaaa
     4081 atgccgaaat ggcacgcagc ttcatgaaat ttgtcgccag cgaagaaggc caggcgattt
     4141 atcgcaaaca cgggtttctg gacaacaaag ccgtcgtaac gcgttgagcc caaccggcct
     4201 ccaggcgccc tggcaatgaa aaccaccagc gatgcagcgc aaagcggcca tgcggcagcg
     4261 gcatccgtcg attcgtcgct gcgctggctg gttggcgtat cgctggtttg tctggtactg
     4321 gtgtttgggt tacgcagcta tttcagcgac cttgagaacg agatcaagca acgcggcgac
     4381 aacgagcgag cccggctgtt tgtcggtgag gaaattgtcc gcggcatcca ggagatcgaa
     4441 aaagacatct accacatggc cgtcacgcaa aattcggccg gtttggcccg tatcaacgcc
     4501 gaaatcgatg gtcgcctggt caagcttgaa catgacttgc aagtgcttca aaacggcggc
     4561 atcagccgcc gacaactgca gctcaacatc gacggcaccg aggaggtcac ccgcgaagcc
     4621 agctaccggc cagacgcggc gaaccagaat gtggtgatgg aactgatcga gattgagcca
     4681 cagctcggtc aaattcgtgc gcgcgccgca atgctgggac agcttttgga gcggcgctgg
     4741 aaaagtcttg agattgaaga taaaaaggca ttcttgagcg ctgaagaaga ggtggtgatt
     4801 gaactcaagc aaataccgcc gcagtttgaa cgtctcaacg aaaacgccaa ccggctgttt
     4861 ctggaaggtg atcaacgttt gcgcgcgctt gaagctgagt tgcaaaggcg cacgagccga
     4921 ctcaaacaga ttgagaccgg actgctcctg atggtggtag tgctgggtgg cgtgctcgcc
     4981 gttttgttca tgcgccgcct gacaggcgcg ctacgggaga cccgccaggc ccgcgatgac
     5041 attgagttgc agcgcgagca aagcgccaac attctcgaca ccctggcaga tggggtctat
     5101 tccactgaca tgaagggctt gatcacctat gtcaatgcag ccggagaacg cattctgggc
     5161 tggagcgcca gggaactgat cgggcagcaa gcgcaccagg cgatccacca cacacggccc
     5221 gacggcacgc ctttttcgcc cgacacctgc ccactgatcg ctgtcgtaga aaaaggcgaa
     5281 acgcttgatg gcgaagacca ctttgtcagc cgcgatgggc agtttgtgcc ggtgtcatac
     5341 cggtccaaac cgctgttgca ggccggtcgg gtggtcgggt cgctgctgtc gtttcacgat
     5401 atcagcgagc gcctggagcg cgaagcccgc atccgactgc aacaggcggc gctggacgct
     5461 gccgtcaaca tgatcgtgat caccagccgc aacggattga tcgagtatgt caatccggcg
     5521 ttttgccgga ccaccggatt tgatgccaac gaagtgctgg gccattcccc cggcatactg
     5581 aagtccggcc agcacgaagc cgggttctac aagaacatgt gggattgcct gctcagcggc
     5641 aaggcgtggg aaggcgagtt gaacaaccag cgcaagaacg gcgaaatcta cccggaacag
     5701 atgaccatta cgccaatttt tgagaaccgg gagattgccc atttcattgc catcaagcgt
     5761 gatatttccg aagagatcag aacccgcacg cgcctcaagc tgattgagtc ggcgattcag
     5821 gaaaccgatc agggcattca catcatggat gccacgccgc acgccttggg tccggttatc
     5881 cagtatgtca atgccggctt tagccgcatc acgggctatg gtgccgacga ggctgtgggt
     5941 gcccgggccg ggttcatgca atcaccgacc actgatccgg gcaagcttgg cctgatcagc
     6001 caggccatgc tgcaaggttc cagcctgacg ctggagatga actaccagcg caaggacggc
     6061 agccgctatg tcggtgaact gcacctgtcg ccggtgcatg cagacagtgg caaggtcagc
     6121 cattacatcg ggctgctgtc agacattgat ctgcgcaagc aagccgagct ggcgctgcgc
     6181 gaggcgcgtg accaggcgtt ggagaactcg cgtctcaagt cggagttttt gtccaccatg
     6241 agccacgaaa ttcgcacccc gatgaatggc atcatcggca tgaccgactt gttgctcgac
     6301 accgagcttg acccggagca gcgcgatttc actggtatcg tgcgcgactc ggcccaggcc
     6361 ttgctggtta tcatcaacga catcctggat ttttccaaga tcgaggcggg caagctcgaa
     6421 attgacatca ccgagttttc ctgctcgcat gtggttgagg gcacgctgga attgctgacc
     6481 tcccgcgcgc acgaaaaaaa gctgacgctg accagttttg ttgacccggc actgccagca
     6541 cgcctgatgg gtgacccaac ccggttgcgt caggttcttc tcaacatgat tggcaatggc
     6601 atcaaattca ccgagtcggg tggggtcgag gtcaatgcgc tgctggtgaa caccggcagc
     6661 acggccatgg tccggtttga gatcaccgac accggtattg gcatttcgca acagacccag
     6721 gccaagctgt ttcagtcatt tacccaggcc gacagttcca ccacgcgcaa gtacggtggc
     6781 accggcctgg ggctggccat ctgcacacgg ctggtcgagc tgatgggtgg tcaagtcggt
     6841 atagaaagta caccagg
//
LOCUS       DAOSLL010000191         6816 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10132_length_6816_cov_5.286496, whole genome shotgun sequence.
ACCESSION   DAOSLL010000191 DAOSLL010000000
VERSION     DAOSLL010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6816
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10132_length_6816_cov_5.286496"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="PLB25_09775"
     CDS             <1..856
                     /locus_tag="PLB25_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HOE41902.1"
                     /translation="NIIEPDCAIITSVDLDHMALLGNDREAIGYEKAGIMRTGKPVVV
                     SDPVPPQSVLDRALEVGADLWRVGVDFNVSGDKQQWGWAGRGRRYSGLAYPALRGANQ
                     LVNASGVLAALSALRDLLPVTAQAVRVGLSSVDLPGRFQIVPGQPTLVLDVAHNPHAV
                     AALTANLDAMGFFPTTHAVFGVMADKDLAAMLPRIKPLIDRWYFTDLPSPRAAQAVHL
                     KQQWQGQNSRKDVSASTHTDPESALQAALAAADPADRIVVFGSFLTVGGVLKDGVPRL
                     AAKHLPTT"
     gene            876..1820
                     /locus_tag="PLB25_09780"
     CDS             876..1820
                     /locus_tag="PLB25_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOE41903.1"
                     /translation="MAFFKFRKGNDETTSAATQPQSIEVIRQRAKYRLVGATVLVLAA
                     VVVLPLLLDSQPRPVAVNTSIDIPDKNKVPALVMAPSLPKPVPTDVVSVAPVAVPEKA
                     PTEKAPTEKAPTEKAPAEKVPTAKAATEKASTPKTPAKTASASEVGIITETAEAPKAP
                     GSSVKKPPQTPVAVAQSASKQVAKVVPKPVPSAAAVAASKPKDADRAQALLDGKSPET
                     KAAAVKDSEPGADAAPPVAGAERFIVQVGAFAENSRAHEVRLKVEHAGLKTYTQVAET
                     KDGKRIRVRVGPFASRAEADKVAEKIRKLDLPAALLAL"
     gene            1945..2433
                     /locus_tag="PLB25_09785"
     CDS             1945..2433
                     /locus_tag="PLB25_09785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HOE41904.1"
                     /translation="MAALDLVFVAVLLLSMLLGTWRGLVYELLSLVNWVAALLLAQWL
                     AQDVAQHLPMRNASEMVRYAAGFVLVFVASVLLGGLVAALARKLISTVGLRPVDRVLG
                     AVFGLLRGMLLLLLATVLVSMTAMKDSAVWQQSVGAGACLVLLKTVKPAMPPEVGRFL
                     PV"
     gene            2461..3960
                     /gene="purF"
                     /locus_tag="PLB25_09790"
     CDS             2461..3960
                     /gene="purF"
                     /locus_tag="PLB25_09790"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HOE41905.1"
                     /translation="MCGIVGIVSQAPVNQLIYDALLLLQHRGQDAAGIVTQQDRKFFM
                     HKARGMVRDVFRTRNMRSLPGNCGLGQVRYPTAGNAFSEEEAQPFYVNAPFGIVLVHN
                     GNLTNAQALKAELFNADHRHINTESDSEVLLNVLAHEIEVTTRGMPLTPGVVFAAVRK
                     VHQRIRGSYAVIALIAGHGVLAFRDPFGIRPLCLGRSDGTWMMASESVALQGTSNQFE
                     RNIAPGEAVFIDLQGTLHAQQCAETASLNPCIFEFVYLARPDSVMDGISVYQARLNLG
                     ESLAKRVISTVPPNEIDVVIPIPESSRPSAAQLAQLLGLPYREGFVKNRYVGRTFIMP
                     GQSVRKKSVRQKLNVIASEFKGRNVLLVDDSIVRGTTSREIVQMARDAGARKVYMASA
                     APPVRFPNVYGIDMPTSQELVAHNRTVDEIRAIIGCDALIYQDVDGMKRAIGSLNLAI
                     KDFDASCFDGVYVTGDITLADIERLNASRVGCDDRQDDNSRLALPNFED"
     gene            3980..5191
                     /locus_tag="PLB25_09795"
     CDS             3980..5191
                     /locus_tag="PLB25_09795"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA];
                     GO_process: GO:0071268 - homocysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HOE41906.1"
                     /translation="MTAKKLPDNLHRDTLAVRAAVPPSQYGENSEALFLTSGYVQPSA
                     EVSARRFAGDEDGFTYGRYGNPTVASFEQRLAALEGAPAAISTASGMSAILMMCMGLL
                     KSGDHVICSHSMFGSTIKLIGTDLAKFGVESSFVPQTEVQAWRAAVRPNTKLLFAETP
                     TNPLTEVCDIKALADIAHNAKALLAVDNCFATPALQRPMALGADIVMHSGTKYLDGQG
                     RVMAGALCASQDLITQTFLPVLKSAGMTLAPFNAWVVLKGLETLDIRMQAQSARALLL
                     AQWLQAHPAVARVHYPGLCSHPQHALAMAQQSNCGGAVLSFEVKAADADQARARAYQV
                     LDALTTLSLCTNLGDTKTLLAHPASTSHGRLTPQQRQLAGVSQGLIRVAVGLEHLDDI
                     QDDLARGLNLF"
     gene            5193..6587
                     /gene="gltX"
                     /locus_tag="PLB25_09800"
     CDS             5193..6587
                     /gene="gltX"
                     /locus_tag="PLB25_09800"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010465783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HOE41907.1"
                     /translation="MTSTTRTRTRFAPSPTGFIHLGNIRSALYPWAFARCTGGDFILR
                     IEDTDLDRSTQAAVDVIIESMSWLGLNYDEGPFYQMHRMDRYRAVLAELVAAGHVYPC
                     FMSMVELDALRDRQTAAKEKPRYDGTWRPAEGKVLPAIPEGVQPVLRFKNPQTGSVEW
                     NDKVKGEIVIRNEELDDLVIARPDGTPTYNFCVVVDDIDMGITHVIRGDDHVNNTPRQ
                     INIFKALGKEPPVYAHLPTVLNELGEKMSKRNGAKPVTQYREEGYLPDAMVNYLARLG
                     WSHGDDEIFSREQFLQWFNLDHLGKSAAQFDEAKLRWVNAQHIKLTPDEVLAPLVRAH
                     LHKRGIEADERLVGICALFKDRCDTTLVLADWAAVFYAEVRPPETELALHVTEAVKPA
                     IRLLADKLAHCVWDKHGIAAAIKEVLQAHRLKMPQLAMPVRILVAGSAHTPSLDAMLG
                     LFVREKVIARLDRV"
     gene            6650..6725
                     /locus_tag="PLB25_09805"
     tRNA            6650..6725
                     /locus_tag="PLB25_09805"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6683..6685,aa:Ala,seq:ggc)
BASE COUNT         1357 a         1959 c         2113 g         1387 t
ORIGIN      
        1 caacatcatc gaacccgact gcgccatcat caccagtgtc gatctggacc acatggcgct
       61 gctgggcaat gaccgcgaag ccatcggcta cgaaaaagcc ggcatcatgc gcaccggcaa
      121 gccggtagtg gtcagtgacc cggtgcctcc ccaaagtgtg ctggaccggg cgctggaagt
      181 gggcgctgac ctgtggcggg tcggggtcga tttcaatgtg tcgggcgaca agcagcagtg
      241 gggctgggct gggcgcggca ggcgctacag cgggctggct tacccggcac tgcgcggtgc
      301 caatcagttg gtcaacgcgt caggcgtgct ggcggcgctg tcggcgctgc gcgacctgct
      361 gcccgtcacg gcgcaggcag tgcgcgttgg gctgtcttcg gtggacttgc cggggcgctt
      421 tcagatcgtg ccgggtcagc cgacactggt gcttgatgtt gcacacaatc cgcacgcggt
      481 ggccgcgctc accgccaatc tggatgcgat gggctttttc ccgaccaccc acgcggtctt
      541 tggggtcatg gccgacaagg acctggctgc catgctgcca cgcatcaagc cactgattga
      601 ccgctggtat ttcaccgatt tgccaagccc cagagcagcg caggcggttc atttgaagca
      661 gcaatggcag gggcaaaaca gccggaagga cgtcagcgcc agcacccaca cagaccccga
      721 atctgccttg caggcggcac tggcggctgc tgaccccgct gatagaatcg tcgttttcgg
      781 gtcattcctg actgttggcg gcgtgctcaa ggacggtgtg ccccgccttg ctgccaagca
      841 ccttcctacc acctgattca ctaccactca ccagcatggc ttttttcaag tttcgcaagg
      901 gtaatgatga aaccaccagc gcggccacgc agccgcagag catcgaggtg attcggcagc
      961 gtgccaaata ccgcctggtc ggtgccaccg tgctggtgct ggccgctgtc gtcgttttgc
     1021 cgctgctgct tgacagccag ccgcgaccgg tggcggtcaa cacgtcgatt gacattcccg
     1081 acaaaaacaa ggtgccagcc ttggtgatgg caccgtcgct gcccaagccg gtgcccacgg
     1141 atgtggttag cgttgcgccg gtggcagtgc ccgaaaaggc accaaccgaa aaggcaccaa
     1201 ccgaaaaggc accaacagaa aaggcaccag ccgaaaaggt gccgaccgca aaggcagcga
     1261 ccgaaaaggc atcgacccca aagaccccgg ccaagaccgc ttcagcgtca gaagtcggga
     1321 tcatcacgga aactgccgag gcccccaagg cgcccggcag ttcagttaaa aaaccccctc
     1381 aaacgcccgt ggcagtagcg cagagcgctt ctaaacaagt agcaaaagtg gtacctaagc
     1441 cagtcccgtc agccgctgct gtggccgctt caaagcccaa ggatgctgat cgcgcgcagg
     1501 ccctgctgga cggcaaatcc cctgagacca aagcggctgc ggtcaaagac agcgagcctg
     1561 gcgcggatgc cgcgccgccg gttgccgggg ctgagcgctt tatcgtgcag gtgggtgcgt
     1621 ttgccgagaa ctcccgtgcg catgaggtgc gcctgaaggt ggaacatgcg ggcctgaaga
     1681 cttacacgca ggtggctgaa accaaggatg gcaagcgcat tcgggtgcgg gtgggtccgt
     1741 ttgccagcag ggcagaagct gacaaggtgg ccgaaaaaat cagaaaactc gatctgccgg
     1801 cggcgctgtt ggcgctttag tgccattttt aaaccatcca tgtcgtcgtt atctcgccaa
     1861 gtcttgcgtt tgcaatgtct gcaccagggt tcccgaacac gagtggttta ggcgtggcat
     1921 gagcacccgc cccgcacttt gctgatggcc gcgctggacc tggtttttgt cgcggtgttg
     1981 ctgctgtcga tgctgttggg cacttggcgc ggcttggtct acgagctgct gtcgctggtg
     2041 aactgggtgg ctgcgttgct gcttgcgcag tggctggcac aagatgttgc acagcacctg
     2101 ccgatgcgca atgccagcga gatggtgcgc tacgcagctg gttttgtgct ggtgtttgtt
     2161 gccagtgtct tgctcggcgg tctggtggct gcgctggcaa gaaagttgat cagcacggtg
     2221 ggtttgcggc cggttgaccg ggtgctgggt gccgtttttg gcctgctgcg cggcatgctg
     2281 ctgctgttgc tggccaccgt gctggtcagc atgacggcaa tgaaggacag tgctgtctgg
     2341 cagcaatcgg ttggtgccgg ggcttgcctg gtgttgctga aaaccgtcaa gccagcaatg
     2401 ccaccagaag tcggccgttt tttgccagtt tgaatgtgtt gttgaacgga tgggaaattt
     2461 atgtgtggaa tcgtagggat tgtcagtcaa gcgccggtca atcaattgat ctacgatgcc
     2521 ttgttgcttt tgcagcaccg cggacaggac gctgcgggca ttgtcacgca acaggatcgc
     2581 aagtttttca tgcacaaggc acgcggcatg gtgcgcgacg tgtttcgcac ccgcaacatg
     2641 cgttcgctgc ctggcaactg cgggctcggg caggtgcgtt acccgacggc aggcaacgcc
     2701 ttcagtgaag aagaagcaca gccgttttat gtgaacgcgc cgtttggcat tgtgctggtg
     2761 cacaacggca acctgaccaa tgcgcaggcg ctcaaagccg agttgttcaa tgccgatcac
     2821 cgtcacatca acaccgagag cgactccgag gtgctgctca acgtgctggc ccatgaaatt
     2881 gaggtcacca cccgtggcat gccgctgacc ccgggcgttg tctttgcggc agtgcgcaag
     2941 gtgcaccaac ggattcgcgg ctcctatgcg gtgattgcct tgattgccgg gcatggggtg
     3001 ctggcgtttc gagacccgtt tggcattcgg ccgctgtgcc tcgggcgcag tgacggcacc
     3061 tggatgatgg ccagcgagtc ggtcgcgctg cagggcacat cgaaccagtt tgagcgcaat
     3121 attgcgccag gcgaggcagt tttcatcgac ttgcagggca cgctgcacgc ccagcaatgt
     3181 gccgaaacag cgtcgctcaa cccgtgtatt ttcgagtttg tttatctggc caggccggac
     3241 tcggtgatgg atggcatttc ggtctatcag gctcgcctta acctgggtga gtccttggca
     3301 aaacgggtga tttccaccgt gccgcccaac gaaattgatg tggtcatccc gatccccgag
     3361 tccagtcgcc ccagtgcggc gcaactggcg cagttgctgg gcttgccgta ccgcgaaggt
     3421 tttgtgaaaa accgctatgt cgggcgcacc ttcatcatgc cgggtcaatc ggtgcgcaaa
     3481 aaatcggtgc gccagaagct caacgtgatc gccagcgagt tcaagggccg caatgttttg
     3541 ctggtcgatg actcgattgt gcgcggtacc accagccgcg agatcgtgca gatggcacgc
     3601 gatgccggtg cgcgcaaggt ttacatggcc agcgccgcgc caccggtgcg tttccccaat
     3661 gtgtacggca ttgacatgcc aacatcgcag gaactggtgg cgcacaaccg cacggttgat
     3721 gagattcggg cgatcattgg ctgcgatgcg ctgatttatc aggatgtgga tggcatgaag
     3781 agagcgatcg gctcgctgaa cctggccatc aaggactttg atgcatcatg ttttgacggg
     3841 gtttatgtta ccggcgacat cacgctggca gacatcgaac gcctgaacgc gagccgggtc
     3901 ggctgtgatg atcgtcagga tgacaactcc cggctggcct tgccaaattt cgaagactga
     3961 agccaaaagc acaattcata tgacagcaaa gaaattacct gacaacctgc accgcgacac
     4021 gctggcggtg cgcgccgccg tgccgcccag ccagtatggc gagaattcag aggcgctttt
     4081 tctgaccagc ggttatgtgc aacccagcgc tgaagtcagc gcccggcgct ttgccggcga
     4141 cgaagatggc tttacctacg gccgctacgg taacccgacg gtggccagtt ttgagcagcg
     4201 actggctgca ctcgaaggcg caccggcggc catttccacg gcatcgggca tgtccgccat
     4261 tctgatgatg tgcatgggct tgctgaaaag tggcgatcat gtgatttgtt cgcactcgat
     4321 gttcggttca accatcaagc tgatcggcac cgatttggcc aagtttggtg ttgaatcaag
     4381 ctttgtgcct cagaccgagg tgcaggcctg gcgtgccgcc gtaaggccca acaccaaact
     4441 gctgtttgcc gaaaccccga ccaacccgct taccgaggtg tgcgacatta aggcgctggc
     4501 cgacatcgcc cacaatgcca aagccttgct ggccgtcgat aactgttttg ccacgccggc
     4561 cttgcagcgc ccgatggcgc tgggtgccga tatcgtcatg cattcaggca ccaaatacct
     4621 cgacggccag ggccgtgtca tggccggggc gctgtgcgcc agccaggacc tgatcacaca
     4681 aactttttta ccggtgctaa agagtgcagg gatgacattg gcaccgttca acgcctgggt
     4741 ggtcctgaaa gggctcgaga cgcttgacat ccgcatgcag gcgcagtcag cccgggcgct
     4801 gctgctggcg cagtggctgc aggcccatcc ggcggtggcg cgggtgcact acccgggctt
     4861 atgcagccat ccgcagcacg cgctggcgat ggcgcagcag tccaattgcg gtggcgctgt
     4921 gctgtcattc gaagtcaaag cggccgatgc tgatcaggcg cgcgcgcgcg cataccaggt
     4981 acttgatgca ctgacaacgc tgtcactgtg caccaacctg ggtgacacca aaaccctgct
     5041 ggcgcacccg gccagcacgt cgcacggccg cttgacaccg cagcagcgcc agttggctgg
     5101 tgtcagccag gggctgattc gtgtagccgt tggccttgaa cacctcgacg acatccagga
     5161 cgatctggct cgtggcctaa accttttctg atatgacctc aactacccgt acccgaaccc
     5221 gtttcgcacc ttctcccacc ggtttcatcc acctcggaaa catccgctcg gcgctgtacc
     5281 cgtgggcgtt tgcccgctgc accggtggcg acttcatctt gcgcatcgaa gacaccgatc
     5341 tggatcgctc gacccaggcg gcagtggatg tcatcattga gagcatgagc tggctcggcc
     5401 tgaactatga cgaaggtccg ttctatcaaa tgcaccgcat ggatcgctac cgggcggtac
     5461 tggctgaact ggtggcagcg ggccatgttt acccatgttt catgagcatg gtggagcttg
     5521 atgcgctgcg tgaccgccag actgctgcca aagagaagcc gcgctacgac ggcacctggc
     5581 gtccggccga gggcaaggtg ctaccggcca ttcctgaagg tgtgcaaccg gtgctgcgtt
     5641 tcaaaaaccc tcaaaccggt tcagtcgagt ggaacgacaa ggtcaaaggg gagatcgtga
     5701 tccgcaacga agagctggac gacctggtga ttgcccgacc cgacggcacg cctacctaca
     5761 acttctgtgt ggtggtggac gacatcgata tgggcatcac ccatgtgatt cgaggcgatg
     5821 accacgttaa caacacgccg cgccagatca atattttcaa ggccttgggc aaggagccgc
     5881 cggtctatgc ccacctgccc acggtgctca acgagctggg cgaaaagatg agcaagcgca
     5941 atggggccaa gccggtgacg cagtaccgcg aagaaggcta tctgcctgat gccatggtga
     6001 actatctggc gcggctgggc tggagtcacg gtgatgatga gatctttagc cgggagcagt
     6061 ttttgcaatg gttcaacctc gatcatctgg gcaagagcgc ggctcagttc gacgaagcca
     6121 agctgcgctg ggtcaatgcc cagcacatca agcttacccc cgacgaggtg ttggcaccgt
     6181 tggtgcgagc gcatttacat aaacgaggca tagaagccga cgaacgcttg gttggcatct
     6241 gtgcgctgtt caaggaccgc tgtgacacca cgctggtgct ggcagactgg gccgcggtgt
     6301 tttatgccga ggttcgaccg ccagagactg agctggcctt gcatgtcaca gaggccgtca
     6361 aaccagccat tcgtttgctg gcagacaagt tggcgcactg cgtttgggac aagcacggta
     6421 tcgctgcagc catcaaggaa gtattgcaag cgcaccgttt gaagatgccg cagttggcca
     6481 tgccggtgcg tatattggtc gcgggcagcg cccatacgcc ttcgctggac gcgatgctcg
     6541 ggctgttcgt gcgtgaaaaa gtcatcgcaa ggttggatcg ggtttaattt tcacgctaga
     6601 atgcaaggct tggaaattgc aggcagccta agaactgtgt tgtggttttg ggggtatagc
     6661 tcagctggga gagcgcttgc atggcatgca agaggtcagc ggttcgatcc cgcttacctc
     6721 caccaaattt ccaaattgag atgaaagtct ataggttttg accccatcgt ctagaggcct
     6781 aggacactac cctttcacgg taggtaccgg ggttcg
//
LOCUS       DAOSLL010000192         6792 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10173_length_6792_cov_5.116521, whole genome shotgun sequence.
ACCESSION   DAOSLL010000192 DAOSLL010000000
VERSION     DAOSLL010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6792
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10173_length_6792_cov_5.116521"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="PLB25_09810"
     CDS             <1..680
                     /locus_tag="PLB25_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HOE41908.1"
                     /translation="LIDFVACDARYAKHFARVPRAHWSATLAPAGDVVAQEGQGQPGN
                     VPCLLMVDPDNRLQKLIVDEKLIREARRCRSMWNSLQELGGIHNSHAERLLAQERKTW
                     AQDAQNTVVVAAPVEPAASAALATPPVGVAASAVEPEVQRSPDEAYIETSRCSTCNEC
                     TQINSKMFAYDGNRQAYIADVTAGSYAQLVEAAENCQVSIIHPGKPRNPDEPGLDELQ
                     KRAELFA"
     gene            1095..2987
                     /gene="uraD"
                     /locus_tag="PLB25_09815"
     CDS             1095..2987
                     /gene="uraD"
                     /locus_tag="PLB25_09815"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HOE41909.1"
                     /translation="MTKPNLQYLNAAPLDEALRLLDGLYEHSPWIARAALQQRPFKSI
                     HQLKHLMTQALDAAGRDAQVALIRAHPELAGKAMVSNSLTAESSNEQSKAGLTHCSAE
                     EFAHLQQLNADYNAKFGFPFILAVRGPRGTGLSRQEIIATFERRLQGQADFELHECLR
                     NIHRIAEIRLNDKFGLTPTLGNLVWDWQERLSEFSEPGYAEQGQLTVTYLSDAHRACA
                     KAIAGWMQDCGFDSVQIDAVGNVVGRYEGRTDEILAPPPDSSEDDSYSRSIDGGYSPK
                     RHIKPQTLLTGSHFDTVRNAGKYDGRLGIFVPMACVRELHRAGRRLPFAIELVAFAEE
                     EGQRFKAAFLGSAALVGDFQPDWLAQLDADGISMQSAMRNAGLCAGTDADLVLGNIAR
                     LKRDPAQYLGFVEVHIEQGPVLNALNLPLGVVSAINASVRYQGEVTGRASHAGTTPMN
                     QRCDAVCGVAELALYLEQRAAQDLDSVATMGQLNVPSGSVNVVPGQATFSLDLRAPND
                     LQRNALERDVLEKLEQICTRRGLCFAVQQTMRASAAPCAPDWQQRWEAAVAALGLPIH
                     HLPSGAGHDAMKLHELMPQAMLFVRGQNAGISHNPLESTTSDDMQLAVEAFSHLLHQL
                     SADNTP"
     gene            3270..5036
                     /locus_tag="PLB25_09820"
     CDS             3270..5036
                     /locus_tag="PLB25_09820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE41910.1"
                     /translation="MEHAPSRPSPQQLTHLAAILDAAELGIWEYDHRSGRMTWSARLC
                     ATIGIAATPTLDFSSWFDRIHPDDCATVQGQMALALQPDGPLYEAEYRFRHGNGHWIW
                     MQSRGRVVERDAAGQPLLTMGTMADITGRKRAQELLRLEHEFSKAINRRPDRETLIDA
                     ILDTALALPELDSGGLYWRGPDGRFELVRQRGLSAEFVAGVSQVAADSPQIQRIATGH
                     MICSCSVPAPHCTDPSLIDQPQYVQEGLLALVVVPILRDQQPIACLNLASHTVRQLDV
                     AVVSAIDALTQQFNLAMQRLLSDEEAAAQACNIAAERQLSDDIINSLPGVFYLFSADG
                     RFLRWNRRFSEVSGYPDAELANMSAADFVIEEDRVALLQAVGEVFKQGVASVGATLLA
                     RDGQTTPYHFSGNRAMVGGQDFLIGVGIDISREVQSRQQLQQVMESAGDAVFVSDLSG
                     QFTFVNATAHALLGYTNGELLRCSIPDVLSPEELAAFPAHAALLEVNPYVRRDWQLKR
                     KDGSALSLELTTQRLPGDRYLAIGRDQSERNLALQQLESERIHLRTLVQTLPDLVWLK
                     DNDGVYLGCNSAFERFFGAKEV"
     gene            5220..5732
                     /locus_tag="PLB25_09825"
     CDS             5220..5732
                     /locus_tag="PLB25_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41911.1"
                     /translation="MAKSGQRKCLNCGDIFDPDCRNVGRQHYCSKPECRRASKAAAQA
                     AWLAKPQNSDYFSGPTQVARVQAWRASNPGYGRARRKVSAALQDTLPLQVIDYIEETT
                     NRGEVPEIPALQDALPPLESMLTGLIAHVFGFALQDDIDMIKARLVQLGSDITYRSHH
                     HEDRQTSAVP"
     gene            5701..6213
                     /locus_tag="PLB25_09830"
     CDS             5701..6213
                     /locus_tag="PLB25_09830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41912.1"
                     /translation="MKIVKQALCPERVRNTPEQFSWVDQALVQHHLIDRCSSHSAALY
                     LFLVTVADCEGLSYYASATLAGRLHLSEAELAAARAQLMALDLIAWQAPLYQVLALPT
                     RRMSASPTSPTVPTQQAATTASPLPRPTRPAQPPASIRPVTPTPNERARSGPLSLAQI
                     LDLERRHAQL"
     gene            6200..>6792
                     /gene="istA"
                     /locus_tag="PLB25_09835"
     CDS             6200..>6792
                     /gene="istA"
                     /locus_tag="PLB25_09835"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HOE41913.1"
                     /translation="MLNYETYCKIRDHLGRQSLTPAQTAQALGLDVRTVSKWADTPQY
                     QPRASTTPRVGKLDDFKGHIVRWLDAHSYSAQQIYQRLCEMGFDGGRTIVKDYVQRIR
                     PRAPQAFLKLDFAPGEAAQVDWGEMGTVGIGSTSRRLSFFLMVLCFSRRMYLEFTVSQ
                     TSEFFLACHERAFAAFGGVPQRLLIDNLKSAVLQRLAG"
BASE COUNT         1349 a         2113 c         1991 g         1339 t
ORIGIN      
        1 cgctgatcga ttttgttgcc tgcgatgcgc gctatgcgaa gcactttgcc cgggttccaa
       61 gagcgcactg gagcgccacc ctggcacccg ccggtgatgt tgtggcgcaa gagggccaag
      121 gccaacccgg caacgtgccg tgtctgctga tggtggaccc tgacaaccgg ctgcaaaagc
      181 tcatcgtcga tgagaaactg atccgcgagg cgcgccgctg ccgcagcatg tggaacagcc
      241 tgcaggaact cggcggcatt cacaattcgc atgccgaacg tttgctggcg caagagcgca
      301 agacttgggc gcaagacgcc cagaacactg ttgtggtggc cgcgccggtt gaaccagcgg
      361 caagtgccgc tttggcaaca ccgccagttg gtgttgctgc cagcgccgtc gagcccgaag
      421 ttcaacgctc gccggatgag gcctacatcg agacctcacg ctgctccacc tgcaacgaat
      481 gcacgcaaat caacagcaag atgtttgcct acgacggcaa ccggcaggcc tacatcgctg
      541 atgtcacggc tggcagctac gcccagttgg tcgaggcagc ggagaattgc caagtctcga
      601 tcatccaccc aggcaagcca cgcaatccag acgagccggg gcttgacgaa ttgcaaaaac
      661 gcgccgagct gtttgcctag cagccggtcg gactcttggt gcaccggcga tgcactgacc
      721 gcagcggccc attttgcgac ggctttcttt tcacctgcaa atgggctgct gaccggcaaa
      781 tccgcaaccc actggcttgg ccgggggcgc tgcctgctgc ccaattcatc ggtccgaccg
      841 tcgccaggcg caccaccggc cacactgtgg cgctgttgta ctggcacctg aagctgcgtc
      901 agcaacccgg tctgaccggc attcgcatgt gacaccgctg cgccggaggt ggctcacctg
      961 cgctgcagca gcgccattgc tggcgacttg ccgacccgcc aggttctgcg caaatcggtg
     1021 catagaatcg cctattcatt gatctggctt cgtggggctg ccaggcgggt gaaggcatcg
     1081 attggggaac aatcttgaca aaaccaaact tgcaatatct caacgccgcg ccccttgacg
     1141 aagcgctcag gctgcttgac ggcctgtatg aacattcgcc ctggatcgcc cgggcagcgc
     1201 tgcaacagcg cccgttcaaa tccatccacc agctcaaaca cctgatgacg caggcgcttg
     1261 acgctgccgg tcgcgatgct caagtggcgc tgatccgggc ccaccccgaa ctggcaggca
     1321 aggccatggt cagcaacagc ctgacggcag aatcaagcaa cgaacaaagc aaggctggcc
     1381 tgacccactg cagcgcagaa gaatttgccc atttgcagca actcaatgcc gactacaacg
     1441 ccaagtttgg ttttccgttc attctggcgg tgcgcggccc gcgcggcacc ggtctgagtc
     1501 ggcaggagat cattgccact ttcgagcgtc ggctgcaggg tcaggctgac tttgagctgc
     1561 acgagtgcct gcgcaacatc caccgcattg ccgaaattcg cctcaacgac aagtttggcc
     1621 tcacgccgac gctgggcaac ctggtgtggg actggcagga gcgcctgagc gagttctcgg
     1681 agcccggcta tgccgaacag ggccagctca ccgtgaccta tttgagcgac gcgcaccgcg
     1741 cctgtgccaa ggccattgcg ggctggatgc aggactgcgg ctttgactcg gtgcagatcg
     1801 acgcagttgg caatgtggtg gggcgttacg aaggtcgcac cgatgagatc cttgcgccgc
     1861 ctccagatag ctctgaagat gatagctact cgcgcagtat tgacgggggc tacagcccta
     1921 aaagacatat aaaaccacaa accttgctga ccggcagcca tttcgacacg gtgcgcaacg
     1981 cgggcaagta cgacggtcgg ctgggcattt ttgtgccgat ggcctgcgtg cgtgaactgc
     2041 accgtgccgg gcgtcgcctg ccatttgcca ttgaactggt agcgtttgcc gaagaagaag
     2101 gccagcgctt caaagctgcc tttctgggct cggcggcact ggttggggac ttccagcccg
     2161 actggctcgc tcaactcgat gccgacggca tctcgatgca gagcgccatg cgcaacgccg
     2221 ggctgtgcgc tggcaccgat gctgatctcg tgctgggcaa cattgcccgc ctcaagcgcg
     2281 acccggcgca gtacttgggc tttgtcgagg tgcatatcga gcaggggccg gtgctcaacg
     2341 cgctgaacct gccactgggc gtggtcagcg ccatcaacgc cagtgtgcgc tatcagggcg
     2401 aggtgaccgg ccgcgccagc catgccggca caaccccgat gaaccagcgc tgtgatgcgg
     2461 tttgcggcgt ggccgaactg gccttgtatc tggaacaacg ggccgcgcaa gaccttgact
     2521 cggtggccac catgggacag ctcaatgtgc cgtctggcag tgtcaacgtg gtgccggggc
     2581 aggccacctt cagtctcgat ttgagggctc caaacgacct gcagcgcaat gcccttgagc
     2641 gcgacgtgct agaaaaatta gagcaaatct gcacccggcg cggcctgtgc tttgccgtgc
     2701 agcaaaccat gcgggccagc gccgcaccct gtgcgccaga ctggcagcag cgctgggaag
     2761 ccgctgtcgc cgcacttggc ctgccgattc accatctgcc cagtggtgcc ggtcacgatg
     2821 cgatgaaatt gcatgaactg atgccgcaag ctatgctgtt tgtgcgcggc cagaacgccg
     2881 gcatcagcca caacccgctc gaatcgacca ccagcgacga catgcaattg gcggtcgagg
     2941 cgttctcgca tttgctccac caactaagcg cggacaacac tccttgacaa gctgtcgcac
     3001 tttcggtgtc aggcaaaaat aacttgaaca ggtcggccgc actggccagc gccatgcgtt
     3061 gttgcaagcc gctggcctag ccatttgcac cgcgcggcgt gcgcctggca ctgcacttgc
     3121 cagcacgacc acatttgtcc atttgttctc gcttgaccca gtccagatga caacaaatgg
     3181 tcaactgagg aatccgcgtt gatgcagtac tgtcgctgca ccaaggtgcg atacttggtt
     3241 gtgtgtcatt gaaaggccta gccgtgcaca tggaacatgc cccatcccga ccgtcgcccc
     3301 agcaattgac ccaccttgca gcgattcttg atgccgccga actgggcatt tgggaatacg
     3361 accaccgcag cgggcgcatg acatggagtg cacgattgtg cgcaaccatt ggcatcgctg
     3421 ccacgcccac gctggatttt tcgtcctggt ttgaccgcat tcatccggat gactgtgcga
     3481 cggtgcaggg ccagatggcg ttggcactgc agcctgacgg ccccctttat gaggcagagt
     3541 atcggttccg ccacggtaac ggtcactgga tctggatgca aagccggggc cgggtggtgg
     3601 aacgtgatgc cgccgggcag ccgctgttga cgatgggcac catggccgac atcaccggcc
     3661 gcaagcgtgc ccaggagcta ttgcggctgg agcacgagtt ctccaaggcg atcaaccgtc
     3721 gccccgaccg cgaaacactg atcgatgcca tactggacac ggcgctggcg ttgccggaac
     3781 tcgattcggg tgggctctat tggcgcgggc cggacggccg gttcgaactg gtacgccagc
     3841 gcggcttgtc ggccgagttt gtggccggtg tcagccaggt tgcggctgac tcgccgcaaa
     3901 tccagcgcat tgccacaggt cacatgatct gtagttgcag cgtgccggca ccgcactgca
     3961 ccgatccgag cctgattgat cagcctcaat acgtccagga gggtctgctg gccctggtgg
     4021 tggtcccgat tttgcgtgac cagcaaccca tcgcctgcct caatctggcc agccatacgg
     4081 tgcgccagct tgatgtcgcc gtggtttctg cgattgatgc cctgacgcag cagttcaacc
     4141 tggccatgca acgcctgctc agtgacgagg aagccgccgc acaggcgtgc aacatcgctg
     4201 ctgaaaggca gctttccgac gacatcatca acagcctgcc cggtgttttt tacctgtttt
     4261 ccgccgacgg tcgttttctc cgctggaacc gacgtttcag cgaggtgagt ggataccccg
     4321 acgctgaact ggccaacatg agcgcagccg acttcgtgat cgaagaggat cgggtcgcac
     4381 tgctgcaggc ggtcggagag gtcttcaagc agggcgtggc cagtgttggg gccaccctgc
     4441 tggcgcgtga cggtcaaacc acaccgtacc acttttccgg caaccgggcg atggtgggag
     4501 ggcaagactt tttgatcggt gtcggcatcg acatcagccg cgaagtgcag tcacgccagc
     4561 aactgcagca ggtcatggaa agtgccggtg atgccgtgtt tgtcagcgat ctgtcgggcc
     4621 aatttacctt tgtaaatgcc acagcgcatg cgctgcttgg ttataccaat ggtgaactgt
     4681 tgcgttgcag cattcccgat gtgttgtcac ctgaagaact ggcagctttt ccggcccatg
     4741 cggcactgct ggaggtaaac ccctacgtgc ggcgcgactg gcagctcaaa cgcaaggacg
     4801 gcagtgcgct gtcactggaa ttgaccaccc aacgcctgcc gggggaccgt tatctggcca
     4861 tcggtcgcga tcagtccgaa cgcaatttgg ccctgcaaca gctcgaatca gagcggattc
     4921 acctgcgcac gctggtgcag actttgcccg acctggtttg gctcaaggac aacgatggtg
     4981 tctacctggg ctgcaattcg gcttttgaac gctttttcgg tgccaaggaa gtgtgaccgc
     5041 cgactcgaaa ctgcgtgttt tcagccggaa tgaacgtgta ttttcttctg catccgtttt
     5101 ctttcagaat tgtttttaac gcgtcttctt ctgccccttt ttcgttttct tcacacttcg
     5161 cctgagggcg tgcaagcggc cattggggtt cttcttctcg acctgatcga ggagtgtcca
     5221 tggccaagag cgggcaacgc aagtgcttga attgcggcga catttttgat cctgactgtc
     5281 gcaacgtggg gcgacaacac tactgcagca aacccgaatg ccgccgtgcg agcaaggctg
     5341 cagcccaagc cgcctggctg gccaaaccac aaaacagcga ctacttttcc ggccccaccc
     5401 aagtggctcg ggtgcaagcc tggcgtgcct ccaaccccgg ctatggccga gcgcggcgca
     5461 aggtctcagc tgcgttacaa gataccttgc cattgcaagt aattgattac attgaagaaa
     5521 ctaccaatcg tggtgaggtg cctgagatac ctgcgttaca agatgcctta ccccccttgg
     5581 agtcaatgct gacggggctt atcgcccatg tgttcggctt tgcgttacaa gatgacatcg
     5641 acatgatcaa ggctcgcttg gtacaacttg gctctgacat cacctaccgg agccatcatc
     5701 atgaagatcg tcaaacaagc gctgtgccct gagcgcgtgc gcaacacccc cgaacaattt
     5761 agctgggttg accaagcgct ggtacagcac cacctgattg accgctgtag cagccacagc
     5821 gcagcgctgt acctgtttct cgtgaccgtc gccgactgtg aggggctgag ctactacgcc
     5881 agcgccacac tggcgggccg actgcacctc agtgaggcgg agttggccgc tgcgcgcgcc
     5941 cagctgatgg cgctcgatct cattgcctgg caggccccgc tgtaccaagt gctggccttg
     6001 ccaaccagac gtatgtccgc ttcacccacg tcaccgacag tacccacgca gcaagcggcc
     6061 acgacggcat caccactgcc ccggccaacg cgaccagcgc agcccccagc gtcaatacga
     6121 ccagtaacac ccacacccaa cgaacgagct cgcagcggcc ccttaagtct ggcccaaatc
     6181 cttgacctgg agcgccgtca tgctcaacta tgaaacctac tgcaagattc gtgaccacct
     6241 gggccgtcaa agcctcaccc ccgcccagac cgcgcaggcg ctgggtctgg atgtgcgcac
     6301 cgtctccaaa tgggctgaca ccccgcagta ccaaccccga gcgagcacca caccacgggt
     6361 gggcaagctc gatgatttca aaggccacat cgtgcgctgg ctcgatgccc actcctacag
     6421 cgcccagcaa atctaccagc gcctgtgtga aatgggcttt gatggcgggc gcaccattgt
     6481 caaagactac gtgcagcgca tccgtccacg cgcaccccaa gcctttctca agcttgactt
     6541 cgcaccaggt gaggcggccc aagtggactg gggcgagatg ggcacggtgg gcatcggcag
     6601 tacaagtcgg cgtttgagct tttttctgat ggtgctgtgt tttagcaggc gcatgtacct
     6661 ggagttcacc gtctcgcaaa ccagtgaatt ctttctggcc tgtcatgagc gggcgtttgc
     6721 cgcctttggc ggcgtgcccc aacgcttgtt aatcgataac ctgaagtccg ccgtgttaca
     6781 gcgcctggcc gg
//
LOCUS       DAOSLL010000193         6786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10183_length_6786_cov_4.297430, whole genome shotgun sequence.
ACCESSION   DAOSLL010000193 DAOSLL010000000
VERSION     DAOSLL010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10183_length_6786_cov_4.297430"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="PLB25_09840"
     CDS             complement(<1..1101)
                     /locus_tag="PLB25_09840"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE41914.1"
                     /translation="MLPAALPAFVGCTGGATVQPAMQALITELRRRVALWRAEHELVA
                     HKAHRLNLQRARSVAPMVAAISAVFAAWLGIELLGQHDSDLVTRWKLGLFLTHGLMAI
                     SFGLLAWAAHHLQHAVKSFMGKALPLLMVALALLFVIGFVTFDQWVTPNITAFLLGLM
                     AVSMVVHLRPSSSGWIFAAAYLLFFFTIGLTQGNPEQLLSNRINGLAAVGFGWGLSVL
                     LWRQFTTLTLQQEQLESANVELQNKQKELQRLTRLDGLTGLYNRNTFVELTRQELARA
                     QRQGSGTTILLLDLDFFKRVNDTWGHPAGDAVLKNVAAVANSTVRATDLVGRLGGEEF
                     IILLPSTSLEAGRKLAEKLRANLQHTPTPWQQG"
     gene            complement(1137..2252)
                     /gene="ald"
                     /locus_tag="PLB25_09845"
     CDS             complement(1137..2252)
                     /gene="ald"
                     /locus_tag="PLB25_09845"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012365921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HOE41915.1"
                     /translation="MLIGLPKEIKNHEYRVGLTPASVRELTTHGHQVLVQTGAGASIG
                     LLDAQYQAAGAKLAASAAEVFAAADMIVKVKEPQPQECSMLRPGQILYTYLHLAPDPD
                     QTAALVKSGAVCIAYETITGPGGGLPLLAPMSEVAGRMAIQAGAAHLEKSKGGMGVLL
                     GGVPGVPAAHVVILGAGVVGTHALQMAVGMGARVSVLDKNMDRLRQLDLIFGNRIHTI
                     FSTAQSVEDAVLDADLVVGGVLIPGAAAPKLVSRAMISRMKKGAVVVDVAIDQGGCFE
                     TSHATTHAEPTYLVDGVVHYCVANMPGAVARTSTFALNNATIGHAVALADKGWKQALL
                     DNPHLLAGLNVCNGQVTYEAVAKDLGYAYVAADTLLS"
     gene            complement(2432..5407)
                     /locus_tag="PLB25_09850"
     CDS             complement(2432..5407)
                     /locus_tag="PLB25_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE41916.1"
                     /translation="MRVLLYKDLNTRRVKAAFAKVRAALEADDFRSADVKKLAPTHYW
                     RAKLDYSNRLLLQFVRYGTETVCLALEVIENHAYEKSRFLRGATLDEAKIEQESVPKD
                     SASVHNLADTQPMRWLHASRSEFELLDKPIMFDDAQEAVRRLPAPVVVVGSAGSGKTA
                     VTLAKLREASGRVLYVTLSSYLAQSARALYDAHGFENPAQEAEFLSYREFVETLRVPP
                     GREVSFTAFAGWFERHRQTSRAALGELDAHALFEEFRGVISATAQGPLDLPGYLALGP
                     RQSLLSPAAREVAHTLFQRYRQWLVEAHLFDLNLVAFEWQPLAQPVYDFVVIDEVQDL
                     TNVQLALVLACLKKPGEFLLCGDSNQIVHPNFFSWAAVRTLFWHGLAGEAAQRQTLSV
                     LQANFRNTHSVTELANTLLKIKQARFGSIDRESNFLVQSTSQTEGLVSLLPARDTALR
                     ELDNRTRASVQHAVVVLRDEDKPAAREHFRTPLVFSVHEAKGLEYPHVILYALVSGQR
                     AAYAEVCSGVSGADLTVQELSYNRARDKGDKSLELYKFYVNALYVAMTRAVESLTLVE
                     SDTDHPLLELLGLRVAAAAMAQATPVQTSSREEWAQEARKLELQGKEEQARAIREAFL
                     QVKTVPWTPWSEPLIRELLPRALDRALPSNKPRQVLMDYALWHGQQAWVEKLATQTQF
                     QPARSLVPGDIYGQVKSGEYRGSYYDDYAGDFERQRQLGYRAASSLRQRHLQPYAAHN
                     FKDLLRQCDSYGVDHPTPTGGTTLMLAARAGNLALVDALLLRGADPQAQDEFGHTAWM
                     TALNRAIDDADFARQKIAALFDRIGPPVLDVQTDGRLVRLERHQGEYWLLSLMLAGLK
                     TLRSRCVARSQHPYKYESGFFAEGLQTTLECLPEHLWKTQRRKRSYVDAVLARAEVQS
                     QYQPARRLWLRTKNGHYLPNALLQLRTDSSDGPGWTPLPQVLNVPWVDDGTYRPDRWV
                     KSLARLLDPTQVAQDDEVF"
     gene            5590..5850
                     /locus_tag="PLB25_09855"
     CDS             5590..5850
                     /locus_tag="PLB25_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41917.1"
                     /translation="MRLWSLFDVVEKCLNQAEGERVKRIFRRAQYERPMGDAWRLCSD
                     RPSLLPLSAASEATNVVQGPHRSSRQIPSSQSVLTAQKSGIA"
     gene            complement(6387..>6786)
                     /locus_tag="PLB25_09860"
     CDS             complement(6387..>6786)
                     /locus_tag="PLB25_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE41918.1"
                     /translation="ASAYPFGKLSDRMSHSTILAGGLVVLIAADLVLASGSHWMTLLG
                     GVALWGIHMGMTQGLLATMVADTAPADLRGTAYGVFNLVSGLSMLLASGVAGWLWDRH
                     GAATTFLGGAAFAGIALVGLLTRPARFASK"
BASE COUNT         1359 a         2086 c         2042 g         1299 t
ORIGIN      
        1 gccttgctgc cacggggtgg gggtgtgctg caggttggcg cgcagctttt cggccagctt
       61 gcgccctgcc tccaatgagg tgctgggcag caagatgatg aattcctcgc cgccgaggcg
      121 accgaccaga tcggtcgctc gcaccgtgct gttggcgact gcggccacat ttttaagcac
      181 cgcgtcgccc gccggatgac cccaagtgtc gttgacgcgc ttgaaaaagt ccaggtccag
      241 caacaagatg gtggtgccag aaccctggcg ctgagcgcgt gccagttcct ggcgggtcag
      301 ctcgacaaag gtgttgcggt tgtaaagccc ggtcaggccg tccagccggg tcaggcgctg
      361 caactccttt tgcttgttct gcaactcgac attggcactt tcaagttgct cttgctgcag
      421 cgtcagggtg gtgaactggc gccacaacaa gaccgacaaa ccccagccaa aaccaactgc
      481 cgccagcccg ttgatgcggt ttgacagcaa ctgctccggg ttgccctggg tcaggccgat
      541 ggtgaaaaaa aacagcaggt aagccgctgc aaaaatccag ccagagctgc tggggcgcag
      601 gtgcaccacc atgctgacgg ccattaaacc cagcaaaaag gccgtgatgt tgggcgttac
      661 ccattggtcg aaggtgacaa acccaatcac aaacagcaag gccagcgcga ccatcagcag
      721 cggcagtgcc ttgcccatga agctcttgac cgcgtgttga agatggtgcg cggcccaggc
      781 cagcaagcca aagctgatcg ccatcagccc gtgggtcaga aacaggccga gcttccatcg
      841 cgtcaccaga tcgctgtcat gctggccgag caactcgatg ccaagccacg cggcgaacac
      901 cgcactgatg gcggcgacca tcggtgccac gctgcgtgcg cgctgcaggt tcaggcgatg
      961 cgctttgtga gctaccaact cgtgctctgc gcgccaaagg gcgacacggc ggcgaagttc
     1021 agtgatcagt gcttgcatgg cgggctgaac tgtagcacca ccggtacaac cgacaaaggc
     1081 gggcagggcg gctggcaaca tggccgcttg cgcgcagccg tgctgccgtt gtgcgatcag
     1141 ctcagcagcg tatccgcagc gacataggcg tagcccaggt ctttggcgac cgcttcatag
     1201 gtgacctggc cattgcagac attcagaccg gccaacaggt gcgggttgtc caaaagcgct
     1261 tgcttccagc ccttgtcggc cagcgccacg gcatgaccaa tcgtggcgtt gttgagcgca
     1321 aaagtcgagg tgcgggccac cgcaccgggc atgttggcaa cgcagtaatg caccacgcca
     1381 tcaaccaggt aggtcggttc ggcatgcgtg gtggcgtgcg atgtttcaaa acaaccaccc
     1441 tggtcgattg ccacatcaac caccacggca ccctttttca tgcgcgagat catggcgcgg
     1501 ctaaccagct ttggggccgc agcgcccgga atcaacacgc cgccgaccac cagatcagcg
     1561 tccagcacgg catcttcgac gctttgtgcg gttgagaaaa tggtgtggat gcggttgcca
     1621 aaaatcaggt caagctggcg caggcggtcc atgttcttgt ccagcacgct gacccgcgcg
     1681 cccatgccga ctgccatttg cagcgcgtgg gtgccaacca caccggcacc cagaatcacc
     1741 acatgcgccg caggcacacc aggcacgcca ccgagcaata cgcccatgcc gcccttggat
     1801 ttttcgagat gcgcggcacc cgcctgaatt gccatgcgcc cggccacttc gctcattggt
     1861 gccagcagcg gcagaccgcc acccgggccg gtaatggttt cataggcaat gcacacggca
     1921 ccggacttga ccagtgcggc ggtttgatcc gggtccggtg ccaggtgcag gtaggtgtac
     1981 aaaatctgcc ctgggcgcag catgctgcac tcttgtggct ggggttcctt gaccttgacg
     2041 atcatgtcgg ccgccgcaaa cacctcggca gcgctggcag ccagtttggc acctgctgcc
     2101 tggtactggg catcaagcag gccgatcgaa gccccggctc cggtttgcac cagcacctgg
     2161 tggccgtggg tggtgagttc acgcacactg gccggggtca ggccgacgcg gtattcgtgg
     2221 ttcttgattt ctttgggtag tccaatcaac atggcgggct ctcaatagtt gcgaaagccg
     2281 caagtgtggg acatgcgcac tggcaatgaa acaaagatca gagttttctt cataacattc
     2341 ctgttgctaa tgtttgtgac gttattggcg atttcactta ttgatgaacc gggtcaggaa
     2401 gtggccgcac cattttttgt caatgcgggc atcaaaagac ttcatcatcc tgtgcgacct
     2461 gggttgggtc aagcaacctg gccaggctct tcacccagcg atctggccga taggtgccgt
     2521 catcgaccca cggcacattg agcacctgcg gcagcggagt ccagcctggc ccgtcgctgc
     2581 tgtcggtgcg cagttgcagc aaggcgttgg gcaggtaatg gccgttcttg gtgcgcaacc
     2641 acagccgccg cgctggctgg tattggcttt gcacctctgc ccgcgccagc accgcatcga
     2701 cataactgcg cttgcgccgc tgcgttttcc acaggtgttc gggcaggcat tccagcgtgg
     2761 tttgcagacc ctcggcaaaa aagccggact cgtatttgta ggggtgctgg ctgcgcgcaa
     2821 cgcagcggct gcgcagggtt ttcaagccag ccagcatcag gctgagcagc cagtattcgc
     2881 cctgatgacg ttccagccgc accagtcggc catcggtctg tacgtccagc acaggcgggc
     2941 caatgcgatc aaacagcgcg gctatttttt gccgggcaaa gtccgcatcg tcgatggcgc
     3001 ggttcagcgc ggtcatccag gcggtgtggc caaactcgtc ctgcgcctgc gggtcggcac
     3061 cgcgcagcag caaggcatcg accagcgcca ggttgccagc acgcgcggcc agcatcagcg
     3121 tggtgccgcc ggtcggggtt gggtgatcca cgccatagct gtcgcactgg cgcaacaggt
     3181 ctttgaagtt gtgtgcggcg tagggctgca agtggcgctg gcgcagcgaa ctggcggcgc
     3241 ggtacccgag ttgccgctgc cgctcaaagt cgccggcgta gtcgtcgtag tagctgccgc
     3301 ggtattcccc gctcttgacc tgaccgtaga tgtcgcccgg caccaggctg cgggcgggct
     3361 gaaactgcgt ctgcgtggcc agtttttcca cccaggcttg ctggccatgc cacagcgcat
     3421 agtccatcag cacctggcgc ggcttgttgg acggcagggc gcggtccagt gcgcgtggca
     3481 gcagttcgcg tatcagcggc tcgctccacg gggtccaggg cactgtcttg acttgcagga
     3541 aggcctcgcg aatggcgcgg gcttgctcct ccttgccctg cagttcgagc ttgcgtgcct
     3601 cttgcgccca ttcttcgcgg cttgaagttt gcactggtgt cgcctgcgcc atggcagcag
     3661 cagcaactct caatccgagc agctcaagca gcgggtggtc ggtgtctgac tcaaccaggg
     3721 tcaggctttc caccgcccgg gtcatcgcca catacagcgc attgacgtaa aacttgtaca
     3781 gctcaagcga cttgtcgccc ttgtcgcggg cgcggttgta acttagctcc tgcaccgtca
     3841 gatcagcgcc agacacgccg ctgcagacct cggcataggc cgcgcgctgg cccgacacca
     3901 gcgcatacaa gatgacatgc gggtattcca gccctttggc ctcgtgcaca gaaaacacca
     3961 gcggcgtgcg gaaatgttcc cgcgcggcag gcttgtcttc gtcgcgcagc accaccaccg
     4021 cgtgctgcac cgaggcgcga gtgcggttgt cgagttcgcg cagtgccgtg tcacgcgctg
     4081 gcagcaggct gaccaggccc tcggtctgtg aggtactttg caccagaaaa ttgctttccc
     4141 ggtcaatcga gccaaagcgc gcctgcttga ttttgagcag cgtgttggcc agttcggtga
     4201 cgctgtgggt gttgcgaaag ttggcttgca gcaccgacag ggtctggcgc tgcgcggctt
     4261 caccggccag cccgtgccag aacagggtgc gcaccgcagc ccaggagaaa aagttggggt
     4321 gcacgatctg gttcgagtcg ccgcacaaca gaaattcacc aggttttttc aggcaggcca
     4381 gcaccagcgc gagctgcaca ttggtcagat cctgcacctc atcgatcacc acaaagtcat
     4441 acaccggctg cgccagcggc tgccactcaa aagccaccag gttcaggtca aacagatgcg
     4501 cctcaaccag ccattgccgg tagcgctgaa aaagcgtgtg ggccacctcg cgcgccgccg
     4561 ggctcagcaa tgactggcgc ggccccagcg ccaggtagcc gggcaagtcc agcgggcctt
     4621 gggcagtggc gctgatgaca ccgcgaaact cttcaaacag cgcgtgcgca tcgagctcgc
     4681 ccaaagccgc gcgtgaggtc tggcgatgcc gctcgaacca gccggcaaag gcggtaaaac
     4741 tgacttcgcg cccaggtggc acgcgcaggg tttcgacaaa ttcacggtag ctcaaaaact
     4801 cggcctcctg cgccgggttc tcaaagccat gcgcgtcata cagcgcacgc gccgattgcg
     4861 ccagatagct cgacagcgtc acgtacaaca cccggccgct ggcttcgcgc agcttggcca
     4921 gtgttacggc ggttttgcct gaaccggccg agccgaccac caccaccggc gccggcaaac
     4981 gccgcaccgc ttcttgcgcg tcatcaaaca tgatcggctt gtcgagcagc tcaaactcgc
     5041 tgcggctggc gtgcaaccag cgcatcggct gggtgtcggc caggttgtgg acgctggcgc
     5101 tgtcttttgg caccgactct tgttcaatct tggcctcgtc gagcgtggca ccgcgcagaa
     5161 agcgagattt ttcgtaggcg tggttttcga tgacttcaag cgccaggcaa acggtctcgg
     5221 tgccataacg gacaaattgc agcagcaacc ggttgctgta gtccagcttg gcgcgccagt
     5281 agtgtgtggg agcgagcttt ttgacatcgg cactgcgaaa gtcatcggcc tccaacgctg
     5341 cacgcacctt ggcaaaggct gccttgacac ggcgggtgtt gagatctttg taaagcagga
     5401 cgcgcatggt gagggccagt tgaatgaatt gtcagattat catccgctgc gaaatatgta
     5461 gcatgctgcg cattcacagc aacggctggc ggtcattttg gcatcaaggc tggagattct
     5521 tgcagcgacc gggagccaac tgcacgcgac acaccggttt gacagcatgg agctgctgac
     5581 gctgtcagca tgcgactgtg gtccctcttc gatgtggttg aaaagtgcct gaaccaggcc
     5641 gagggggaaa gggtcaaacg catcttccgc cgtgcccagt acgaacgccc aatgggcgat
     5701 gcctggcggc tgtgcagtga ccggccaagc ctgttgcccc ttagcgctgc aagtgaggcg
     5761 acgaatgtgg tgcaggggcc gcaccggtca tccaggcaaa taccgtcgtc ccaaagtgtt
     5821 ttgacggcgc aaaaaagcgg tatcgcctga tgaatacacg ctgctgccgc caggcggagt
     5881 tttgccactg ccaatcttgc tggtcatcgc cttcaatcga aagatgttcc agtgttgcgg
     5941 aatccgaccg actgcatcaa tgctgcggct ctatgccgtt ttgtgtggag gtcgcgccgg
     6001 aggtctgttt gtggtggcca ctgatgtaga accgctacaa ccagaaaaga ccaacggagg
     6061 ttggcaacgt tcacggtcag tgcctggagt gtgacacctt ttgaaaccag gacttgtcgc
     6121 ctcaaagcct tgattcgaca agcattttcc aattatgaga gccatgcaac cgcatcaggg
     6181 ccaagccctt cgggtggact tcgtccagcc ttgacccggc tgcacggcag acggcaaaca
     6241 ccgaccacgc ccgccagcct gcgtcaagtt ccacacgaaa cgacgaaggg ttcaggaatt
     6301 ccagcagggc aaacctgcaa ttgatttttc aagcccccac ccgtctgaaa tcagcaaagt
     6361 gttttcagca gtcatggagt gccaagtcac ttgctagcga accgggctgg tcgagtgagc
     6421 aatcccacca gtgcaatacc agcaaatgcg gcaccaccca aaaacgttgt agccgcgccg
     6481 tgacggtccc aaagccaacc tgccacaccg ctggccaata gcattgacaa cccgcttacg
     6541 agattgaaaa ccccataggc tgttccgcgc aaatcggcgg gtgccgtgtc ggccaccatg
     6601 gtcgccaaca ggccttgggt catgcccatg tgaatgcccc acagtgcgac accacccagc
     6661 agggtcatcc aatgactacc gctggccagc accaagtcag cagcaatcag aacgaccaaa
     6721 cccccagcca atatcgtgga atggctcatc cggtctgata gcttgccaaa cggataggct
     6781 gatgcc
//
LOCUS       DAOSLL010000194         6768 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10220_length_6768_cov_4.764338, whole genome shotgun sequence.
ACCESSION   DAOSLL010000194 DAOSLL010000000
VERSION     DAOSLL010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6768)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6768)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6768
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10220_length_6768_cov_4.764338"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3013)
                     /locus_tag="PLB25_09865"
     CDS             complement(<1..3013)
                     /locus_tag="PLB25_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HOE41919.1"
                     /translation="MKAPLPETVRQALQSRSLEDKYTLDQGRAFMSGVQALVRLPMLQ
                     QRRDARSGLKTAGFISGYRGSPLGAYDQALVAARKHLEAHHIVFQPGVNEELAATAVW
                     GTQQLDLYPQSKLFDGVFGIWYGKGPGVDRSSDVFKHANMAGTARHGGVLAVAGDDHL
                     SKSSTSAHQSDHIFKACGLPVFFPSDVQDILDLGQHAIALSRFSGVWAGMKTIQEVVE
                     SSSAVQVDLDRVNILMPEDFTLPPGGLQIRWPDPALEQEARLMEYKWYAALAYVRANR
                     LNFNVIASSNDRFGIMASGKAFNDTRQALVDLGLDLATCQRLGIRLHKVNVVWPLEAT
                     LARDFAQGLQEILVVEEKRQIMEYQLKEELYHWRADLRPVVVGKFDDSGDASGEWSHP
                     NPGGNWLLRAKADLNPALVARAIAKRLKKLGVPDDVAARMDRHLDLLDERERGALELK
                     SAGGERTPWFCSGCPHNTSTRVPEGSRALAGIGCHFMAIWMDRSTSTFSQMGGEGVAW
                     VGQQAFTTDQHVFANLGDGTYFHSGLLAIRQSIAAGVNITYKLLYNDAVAMTGGQRVG
                     ERAEGHSVLQIMNSLMAEGVAKLVIVTDAPDKYQGIALAPGVTVQQRDQLDRVQRELR
                     ILKGTTAIIYDQMCATEKRRKRKRGTLSEPDEHIVINELVCEGCGDCSEQSNCLSIEP
                     LETEFGRKRSINQNTCNKDFSCIKGFCPSFVTVKGGQLKKPKVDQRKRPDVLPVLPEP
                     VLPPTGTAWGIVVGGVGGTGVITVGQLLGMAAHLEGKAVVTLDAGGLAQKGGATWSYI
                     QIADHPEAIYTSKVDSAKADLVIGCDAVVAASKTTLSVMQSGRTFVVLNSHATPTAAF
                     VHDPQWQFPQGACAAAIATALGGAEPLAFDAEQIARQALGNAIYTNPLLLGFAWQMGR
                     VPVSRAALLRAMELNGVQVDNNQTAFEWGRHCAHDLRAVQALFAPVHVIELVKNPTLP
                     HMVGRRVAYLTAYQDAAYARCYQSVLDQVQQV"
     gene            complement(3723..4442)
                     /locus_tag="PLB25_09870"
     CDS             complement(3723..4442)
                     /locus_tag="PLB25_09870"
                     /inference="COORDINATES: protein motif:HMM:NF005458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HOE41920.1"
                     /translation="MKCIVLRHLAFEDLGVFAEVLIDQGFEVCYLEAGQHDLTGQNLD
                     QAELLVVLGGPIGVYEANDYPWLRQEIEIVERRLRHDLPTLGICLGAQIMAAALGAAV
                     YPGRDKEIGWSTVRLTPAARGTCLEALEQVAVLHWHGDTYDLPAGATLLASTDLTPNQ
                     AFSFGRNALALQFHAEADGAQMEPWLIGHTVELRQADINIPDLRQAGYRGNVAKVVAG
                     RLMLERWLAQVGLAGACLRIT"
     gene            complement(4439..5035)
                     /locus_tag="PLB25_09875"
     CDS             complement(4439..5035)
                     /locus_tag="PLB25_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003812062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HOE41921.1"
                     /translation="MSHTSFVTSAIASDIAPPLQVAQWFNTDSPITLNAMRGKVVVMH
                     AFQMLCPGCVAQGLPQALRVHRAFARSEVVVLGLHTVFEHHDAMSPVALKAFLHGYRV
                     DFPVAVDLPAPDRPIPRTMQIYELRGTPSLIVIDRQGVIRLNHFGHLDDLQLGALLGA
                     LLTEAVEPPAIGITAPQGRQEPGCDDFHCAVDLAGATS"
     gene            complement(5372..5917)
                     /locus_tag="PLB25_09880"
     CDS             complement(5372..5917)
                     /locus_tag="PLB25_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015063704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HOE41922.1"
                     /translation="MSRVQLINPATTTANRQAVLAQIHKAFGATPNMFKAVANSPAAL
                     KSMWGSFDALGSGVLGAKLGEQIAVAIAERNRCEYCLAAHTVLGVGAGASAEEMSAAQ
                     AGKSNDPKTAAALAFALKVVSSRAQVSASDVAVLRSAGFDDEHIVEIMAHVALNIFTN
                     YVNVAFDVPLDFPKIALTPAA"
     gene            6042..6761
                     /locus_tag="PLB25_09885"
     CDS             6042..6761
                     /locus_tag="PLB25_09885"
                     /inference="COORDINATES: protein motif:HMM:NF024260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOE41923.1"
                     /translation="MMGIFRSVKHTDKSKMNTVIPLALDRLSSLLERSQVSAHLFHTG
                     ALCGVTSFDAQAGRGFVHVLRKGRMVVTHNQGANVPNRIEVNQPTLIFYPQPLTHQFH
                     NAPVDGSDFTCASVHFEGGANHPLVKALPPLIVIPLLEVPTLSGALDLLFQEADQVRC
                     GHRLLADRLFEVVLLLLLRWLLDHGKAHGVSIGLVSGLADPKLARALTAMHESPQLAW
                     SVEGLAGSSSFCVEVALKNKM"
BASE COUNT         1309 a         2099 c         1972 g         1388 t
ORIGIN      
        1 ccacttgctg cacctggtcc agcacagact ggtagcacct cgcataggcc gcatcttggt
       61 aagcggtcag ataggcaacc cgcctgccca ccatatgcgg cagcgtggga tttttgacaa
      121 gttcaatcac atgcaccggc gcaaacaagg cctgcaccgc gcgcaggtca tgggcgcagt
      181 ggcgacccca ttcaaaggct gtctggttgt tgtcaacctg aacgccattg agttccatgg
      241 cgcgcagcag cgccgcccgg ctgaccggca cgcgccccat ttgccaggca aatcccagca
      301 gcagagggtt ggtgtagatg gcgttgccca aggcttgcct ggcgatttgt tccgcatcaa
      361 acgccagcgg ttccgcaccg cccagcgcgg tggcaattgc tgccgcacaa gcgccctgcg
      421 gaaattgcca ttgcgggtca tgcacaaagg ccgctgtcgg cgttgcgtgg ctgttgagca
      481 ccacaaaagt tcgcccggac tgcatcaccg acagggtggt tttgctggcg gccacgacgg
      541 catcgcagcc aatcaccagg tcggctttgg cgctatcgac cttgctggtg taaatcgcct
      601 cagggtggtc ggcaatctgg atgtagctcc aggtggcccc gcctttttgc gccagacctc
      661 ctgcgtcaag cgtgacgacc gccttgcctt ccagatgcgc agccatgccg agcaactggc
      721 caaccgtgat gaccccggtg ccgccaacgc caccgaccac aatgccccag gcggtgccgg
      781 ttggcggcaa gacgggttct ggcaaaaccg gcaaaacgtc gggtcttttg cgctggtcga
      841 ctttgggctt ttttagctgg ccgcccttga cggtcacaaa gctcgggcaa aagcccttga
      901 tgcaactgaa atccttgttg caggtgttct ggttgatgct gcgcttgcgg ccaaattcgg
      961 tttccagggg ttcaatgctc aggcagttgg actgctcgct gcagtcgcca cagccctcac
     1021 acaccagttc gttgatgacg atgtgctcat ctggctcgga cagggtgccg cgcttgcgtt
     1081 tgcggcgctt ctcggtcgcg cacatctggt cataaatgat ggcggtggtg cccttgagta
     1141 tgcgcaactc gcgctgaacg cggtcaagct ggtcacgctg ctgcaccgtt acccctgggg
     1201 ccagcgcaat tccctggtat ttgtccggcg catcggtcac gatgaccaat ttggcaactc
     1261 cttctgccat caggctgttc atgatctgca gcacgctgtg cccctcggca cgttcgccca
     1321 cccgctgacc accggtcatg gcgaccgcat cgttgtacag cagtttgtaa gtgatgttga
     1381 cacccgctgc aatgctctgg cgaatggcca gcaagccact gtgaaaatag gtgccgtcac
     1441 ccaggttggc aaatacgtgc tggtctgttg tgaaggcttg ctgcccgacc caggccacac
     1501 cttcaccgcc catctggctg aatgtgctgg tgctgcggtc catccagatg gccatgaaat
     1561 ggcagccaat gccggccagg gcgcgggaac cctcgggcac gcgggtgctg gtgttgtgcg
     1621 ggcagccgct gcaaaaccag ggggtgcgct cgccgccggc ggatttgagt tccagcgcgc
     1681 cgcgctcgcg ctcatcaagc aaatccagat ggcggtccat gcgcgccgcc acatcgtctg
     1741 gcacgccaag ctttttcagc cgtttggcaa tcgccctggc gaccagcgcc gggttcaggt
     1801 cggctttggc acgcaacagc cagttgccac cggggttggg gtggctccat tcaccgctgg
     1861 catcgcctga gtcgtcaaat ttgccgacca cgaccgggcg caggtcggca cgccagtggt
     1921 atagctcttc cttgagctgg tattccatga tctggcgctt ttcttccacc accagaattt
     1981 cttgcaagcc ctgcgcaaag tcgcgtgcca gggtcgcctc cagcggccac accacgttga
     2041 ccttgtgcag ccgaatgcca aggcgctggc aggtggcaag gtcgagcccg aggtcaacca
     2101 gggcctggcg ggtgtcattg aaggccttgc cgctggccat gatgccaaag cgatcgttgc
     2161 tgctggcgat cacattgaag ttgaggcggt tggcacgcac ataggccaat gcggcatacc
     2221 acttgtattc catcaggcgg gcttcctgct ccagtgccgg gtcgggccat cggatttgca
     2281 gcccgcccgg gggcagggtg aaatcttcgg gcatcaagat gttgacgcga tccaggtcca
     2341 cctgcaccgc gctgctggac tccaccacct cctgaattgt cttcatgccc gcccagacac
     2401 ccgagaaacg gctcaatgca atggcgtgct ggcccaggtc cagaatgtcc tgcacatctg
     2461 acgggaaaaa caccggcagg ccacaggcct tgaaaatatg gtcgctctgg tgcgccgagg
     2521 tcgaactctt gctcaggtgg tcgtcgcccg ccaccgccag cacgccgcca tggcgcgcag
     2581 tgccggccat gttggcgtgc ttgaacacat ctgaactgcg gtccaccccg ggacctttgc
     2641 cgtaccagat gccaaaaacg ccgtcgaaca gtttgctctg cgggtacaaa tcaagctgct
     2701 gggtgcccca cactgcggtc gccgccaact cttcgttgac acccggctga aacacgatgt
     2761 gatgggcctc cagatgcttg cgcgcggcca ccagcgcctg gtcataggcc cccaggggcg
     2821 aaccccggta gccgctgata aacccggccg tcttcaggcc ggatcgggca tcacgccgct
     2881 gttgcagcat tggcaggcgc accagtgcct gaacaccgct catgaaagcc cggccttgat
     2941 caagcgtgta cttgtcttca aggctgcggc tttgaagcgc ctgacgcaca gtctctggca
     3001 atggtgcttt catgaggttt ctccgaagta aaaccggtaa cttgccgacc cttgggcttt
     3061 ggccggtcgg caactcagcc gctaacgttc tgtcaagtgt aggctggcag cagtcattcc
     3121 agctcgaccc tccaggctcg gggcgcgcaa gctggcaagc tgcaatacag catttaagaa
     3181 tgactcaacc tggtcaatgt gttcaccaaa tttggcggaa acccgcccaa cgctgagctc
     3241 accatgtccg tacaggccaa ttgccaccat tgctgctgca agaatgtcaa ggctgcgttc
     3301 accattgatg gcctggccca ggccggcgag ttggttggca cgatccgccg caggctggtt
     3361 tgaaccgcac aaccacgtgt ttggccaagt ccttggtcgc aacagcactc gccgccagtt
     3421 gcgcacgcag gttcaattgg gcagcacgtt caattggccg ccttgacctt tcgccacccg
     3481 atgcgctggt cctccacgtg atccggcacc accccaccac tgccaggtgc ccagtctggg
     3541 tcgcgttgcg ccccgctatg ccttggcgct tcaaggtcgt ccgacaacgg tggcccatct
     3601 gcgggcgatg ttcagggact gcgaggcggc tccgatgtga tggaaaagtc tcctgatctg
     3661 cccgcctcac cctggtcaca gccgctggca gacctcgcag atgcgggcgc tcagcactgc
     3721 cctcacgtaa tgcgcaggca agcacctgca agcccgacct gcgccagcca gcgttcaagc
     3781 atcaatcttc cggcgaccac cttcgcgaca ttgccccggt agccggcttg acgcagatca
     3841 ggtatgttga tgtcggcttg gcgcagctcc acggtgtggc caatcagcca gggttccatc
     3901 tgggctccgt cggcctccgc gtgaaactgc aaggccagtg cattgcgccc aaaagagaag
     3961 gcctggttcg gtgtcaggtc ggtcgatgcc aatagggtcg ccccagcggg caagtcataa
     4021 gtgtcaccgt gccagtgcag caccgctacc tgttccagcg cctccaggca agtaccacgg
     4081 gctgcgggtg tgagccgtac cgtcgaccag ccaatttcct tatcacggcc cgggtacacg
     4141 gccgcaccca atgctgcagc catgatttga gcacccaggc agatgcccaa ggtaggcaag
     4201 tcgtggcgca agcgtcgttc cacgatctcg atttcttgcc gaagccacgg atagtcgttg
     4261 gcctcataca ccccaatggg gccccccagc accaccagca gctcggcctg gtccaggttt
     4321 tgaccggtca ggtcgtgttg cccggcttcc aggtaacaca cctcaaatcc ctggtcgatc
     4381 aaaacctctg caaaaacccc gaggtcttca aaggccagat gccgcagcac gatgcatttc
     4441 atgatgtggc tcccgcaagg tcaaccgcgc aatgaaaatc gtcgcagccg ggctcttgcc
     4501 gaccctgcgg cgcggtaatg ccaattgccg gtggctcaac cgcctcggtc agcagtgccc
     4561 ccagcaaggc acccaattgc aggtcatcca ggtgaccaaa gtgattcagg cggatcaccc
     4621 cctgacggtc tatcacgatc aaacttggtg tgccgcgaag ctcatagatt tgcatcgtgc
     4681 ggggtatggg gcgatctggt gcaggcaagt ccaccgccac cggaaaatca acacggtacc
     4741 cgtgcaggaa ggccttcaga gcaacagggc tcatcgcgtc gtgatgctca aacaccgtgt
     4801 gcaggcccag cacaaccacc tcagagcgag caaacgcccg gtgcacccgc aaggcctgag
     4861 gcaagccctg ggccacgcag cctgggcaca acatctgaaa tgcatgcatg accaccactt
     4921 tgccgcgcat ggcattgagc gtgatgggtg aatcggtgtt gaaccactgg gccacttgca
     4981 gcgggggcgc gatatcgctg gcaatagcgc tggtgacaaa gctagtgtgt gacataggta
     5041 aaacctcatc tatttgccgc tctacgcggt cgccttggca gggggcaaag cgaaattttc
     5101 aaccggggac tgatcctcaa ctttgaggac cttgccattg agaacgagcg atggcaggcg
     5161 catcgtcccc aaggtgcagc gcctctgcgc ctggccttgc gccgggtgcc aaggcaagcg
     5221 acgcataacc accctcccaa tcgcccggca gcgcctgcaa tgtcgccctg atggacaggc
     5281 aaatgttgca ggtaccgtga aagagacatc ttcatggctg tcgcggtggt gttgggtgct
     5341 ggcgggcaca tgccagcgcc cggggaattc gtcaagccgc aggggtcagg gcgatcttgg
     5401 ggaagtccag cggaacgtca aaagccacgt tcacatagtt agtgaagatg ttcagggcca
     5461 cgtgcgccat gatctcgaca atgtgttcat cgtcaaaccc tgcgcttcgc agcactgcca
     5521 catcgctggc agacacctgg gcgcggctgc tgaccacctt cagggcaaaa gccagggccg
     5581 cggctgtctt cgggtcattc gatttgcccg cttgtgccgc agacatttct tctgcactgg
     5641 ctccggcacc cacgcccagc accgtgtggg cggccaaaca gtattcgcag cggttgcgct
     5701 ccgcaatcgc gaccgcaatt tgctcgccca acttggctcc cagtaccccg gaacccaaag
     5761 cgtcaaatga accccacatg cttttcagag ccgctggcga gttggccacc gccttgaaca
     5821 tgttgggcgt ggcaccaaat gctttatgaa tttgcgctag caccgcctgg cggtttgccg
     5881 ttgtggtggc cgggttgatg agttgtacgc gcgacatgat gatttccttt tttcagtgga
     5941 taaagggcaa ctggaccaaa gtgcctttga ggcagcgatt catggtgggt tggtgaatcg
     6001 ctgcaactcg gcacagaacg tatggtgcag gcttgaatcg tatgatgggt atctttaggt
     6061 ccgttaaaca taccgataag tccaaaatga acactgtcat ccccctcgcg cttgaccggc
     6121 tttcatctct gttggagcgt tctcaggtat ccgcccacct ctttcatacg ggcgccttgt
     6181 gcggtgtcac cagctttgat gcccaagcgg gaaggggctt tgttcatgtg ttgcgcaaag
     6241 gtcggatggt ggtcacccat aaccagggcg cgaatgtgcc caatcgaatc gaagtcaatc
     6301 agccaacact cattttttac ccacagccgt taacgcatca gtttcacaat gcgccagtcg
     6361 atggctcaga tttcacctgc gcttccgtcc actttgaggg cggtgccaac catcccctgg
     6421 tgaaggcact gcccccgctg atcgtcatcc ctttgctgga agtgcccaca ctgtcaggcg
     6481 cgctggatct gctttttcag gaggcagatc aagttcggtg tggacacagg ctgctggcag
     6541 atcgtctttt tgaagtggtg ctgctgctgc tgttgcgctg gctgcttgac cacggaaagg
     6601 cgcacggcgt gtcgatcggc ctggtctctg gcttggcaga tccgaagctg gcccgcgccc
     6661 tgaccgccat gcatgagagt ccccagctag catggtcggt agagggtttg gcaggttctt
     6721 cgtcgttttg tgtggaagtt gcattgaaaa ataaaatgta gtagtggg
//
LOCUS       DAOSLL010000195         6751 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10258_length_6751_cov_3.902479, whole genome shotgun sequence.
ACCESSION   DAOSLL010000195 DAOSLL010000000
VERSION     DAOSLL010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6751)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6751)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6751
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10258_length_6751_cov_3.902479"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..367)
                     /locus_tag="PLB25_09890"
     CDS             complement(<1..367)
                     /locus_tag="PLB25_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HOE41924.1"
                     /translation="MSGQNPLPASPLELPLLPLRDVVVFPHMVIPLFVGRPKSIKALE
                     AAMASDRCIMLVAQKAAAKDDPLVTDMFDVGCISTILQMLKLPDGTVKVLVEGQQRAT
                     VGKIDEGESHFTATVTPMEV"
     gene            complement(489..1751)
                     /gene="clpX"
                     /locus_tag="PLB25_09895"
     CDS             complement(489..1751)
                     /gene="clpX"
                     /locus_tag="PLB25_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HOE41925.1"
                     /translation="MAEKKGSSGDKTLYCSFCGKSQHEVKKLIAGPSVFVCDECIDLC
                     NEIIRDELPAKTESVGSNDLPTPSEIKANLDNYVIGQEPAKRILAVAVYNHYKRLRHQ
                     EHSTKDDVELSKSNILLIGPTGSGKTLLAQTLARMLDVPFVMADATTLTEAGYVGEDV
                     ENIVLKLLQSCNYEVERAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIE
                     GTMASVPPQGGRKHPNQDFVQVDTTNILFICGGAFSGLEKVIDNRTESSGIGFGAAVK
                     SKQSRDLTEVFKEVEPEDLIKFGLIPELVGRMPVVATLGELSEETLVQILTEPKNALV
                     KQYSKLLAMEGVDLEIRPPALKAIARKALARKTGARGLRSILELALIDTMFDLPNSSN
                     VERVVVEESTIDENKAPLLVYREIAKKA"
     gene            complement(1757..2377)
                     /gene="clpP"
                     /locus_tag="PLB25_09900"
     CDS             complement(1757..2377)
                     /gene="clpP"
                     /locus_tag="PLB25_09900"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HOE41926.1"
                     /translation="MNSYGGATDVQGLGMVPMVIEQSGRGERSYDIYSRLLKERVIFM
                     VGPVNDHVANLVVAQLLFLESENPDKDISFYINSPGGSVSSGMAIFDTMNFIKPEVST
                     LCTGMAASMGAFLLAAGAKGKRFSLPNSKIMIHQPSGGSQGQATEIEIQAREILKTRE
                     QLNKILAERTGQPLDRIERDTERDYYMSAEESKEYGLIDQVISKRA"
     gene            complement(2515..3825)
                     /gene="tig"
                     /locus_tag="PLB25_09905"
     CDS             complement(2515..3825)
                     /gene="tig"
                     /locus_tag="PLB25_09905"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HOE41927.1"
                     /translation="MPVAVEILEKLERKMTLSLPIGIIQSEVASRLKKLARTVKMDGF
                     RPGKVPMNVVAQRYGYGVQHEVMNEKVGEAFFNAAHEAQLRVAGRPQISEKEGAAEGE
                     LAFEAIFEVFPEVTIGDLATAEVEKVTTEVSDAAVEKTVDILRKQRRTFSQRPHDAAA
                     QDGDRVLVDFEGKIDGEAFEGGKAEDFQFILGDGQMLKEFEEATQGLKLGESKTFPLV
                     FPVDYHGKDVAGKTADFMVTVKKIETAHLPDVDEALAKSLGIANASVDSLRVDIRKNL
                     DREVKNRLLARNKTAVMDALVAKAELDLPNASVRAEMDRLLEGARADLKKRGIKDADK
                     VAIPDDVFRPQAERRVRLGLVVAELVRANDLQVKMEQIKAYVEELAATYEKPAEVSRW
                     YFSDSKKLAEVEGIVLENNVTNFVLSQAKVTEKTITFEELMAQN"
     gene            complement(4456..4989)
                     /gene="lspA"
                     /locus_tag="PLB25_09910"
     CDS             complement(4456..4989)
                     /gene="lspA"
                     /locus_tag="PLB25_09910"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HOE41928.1"
                     /translation="MARKPNLAQATLSRNYKSGSSAGLLPWLAWALLVLMLDQFTKVL
                     IMGYYQLGDSTMVTGFLNVVRVHNSGAAFSFLASAGGWQRWFFTAIGIVAALLMLWLL
                     KLHAGQKLFAFAIASVLGGAVGNVVDRVLHGHVVDFLDFHWAGRHFPAFNVADSAITL
                     GVTCLILDELMRVRRGR"
     gene            complement(5023..>6751)
                     /locus_tag="PLB25_09915"
     CDS             complement(5023..>6751)
                     /locus_tag="PLB25_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOE41929.1"
                     /translation="LREAGRLFATENIVHSYPHCWRHKTPVIYRAAAQWFIRMDAGEG
                     VFTKDKAPKTLRQTALDAIENTAFYPENGKIRLRDMIAGRPDWCISRQRSWGVPLPFF
                     LHKDSGELHPRTMEILDIAADMVQEGGIEAWSKAGTQAILAKVDCAADADHYTKSSDI
                     LEVWFDSGTTHTTVLKGSHPGSGHDDIDPRTGKPGPETDLYLEGHDQHRGWFHSSLLT
                     ACAMYDRAPYKGILTHGFTVDSKGHKMSKSAGNGVDPQETSKKLGAEIIRLWVAASDY
                     SGDIAGDDKILARVVDTYRRIRNTLKFLMANVSDFDPAEDAVPLDQMLEIDRYALARA
                     AALQADILAHYEVYEFHPVVARLQIYCSEDLGSFYLDILKDRLYTTAPKSLARRSAQT
                     ALWQILQAMLRWMAPFLSFTAEEAWAVLGQQGRTPSATRESIFMDSFFSFSDESSGLA
                     RQPELVAKWARIREIREAVNKDIELLRADGKLGSSLQANISLTVGADDHALLASLGAD
                     LKFVFITSTIELIAGDAISISSRASIDPKCERCWHYRDDVGNDNTHPTLCGRCTSNLF
                     GPGEARQFA"
BASE COUNT         1535 a         1965 c         1747 g         1504 t
ORIGIN      
        1 tcacctccat cggcgtgaca gtggccgtga aatgtgattc accctcgtcg atcttgccca
       61 cagttgctcg ctgctgtccc tcgaccagca ctttcaccgt gccatcgggc agtttcagca
      121 tctgcaaaat ggtggaaatg cagccgacat cgaacatgtc ggtcaccaga ggatcatcct
      181 tggcagcagc tttctgggct accagcatga tgcatcgatc tgatgccatg gccgcttcaa
      241 gcgccttgat gctcttgggc ctgccaacaa aaagcggaat gaccatatgg ggaaaaacca
      301 ccacgtcgcg caaaggcagc aacgggagtt ccagcggaga agcaggcaag gggttctgac
      361 cagacataag gggtcctttt gttacctgaa ggaaattggg caagtttgct gcttttcaag
      421 cagcagccca ttatgaataa attggcatgt ttgcgtcggc attaaaaccg atacaaaaac
      481 agatcaaatc aagccttctt ggcgatctct cggtacacca acaaaggagc cttgttctcg
      541 tcgatggttg attcctcaac caccacacgt tcaacattgg acgaattcgg caaatcgaac
      601 atggtgtcga tcagcgccaa ctccaaaatc gaccgaagac cgcgcgcccc ggttttgcgc
      661 gccagtgcct tgcgcgcaat agccttcagc gcgggtggcc gaatctccag atccacacct
      721 tccatggcca agagtttgct gtactgcttg accaacgcgt tctttggctc ggtcagaatt
      781 tgaaccaggg tttcctcact gagttcacct agcgtggcca ccaccggcat gcgtccgacc
      841 aactcgggaa tcagaccaaa cttgatcaaa tcctcaggtt caacttcttt gaaaacctcg
      901 gtcaggtctc ggctttgctt gcttttgact gcggcaccaa agccaatacc ggatgactcg
      961 gtacggttat caatgacttt ctccagcccg gaaaaagcgc caccacaaat aaacaggata
     1021 ttggtggtgt ccacctggac gaagtcctgg ttggggtgct tgcgcccacc ttgaggcgga
     1081 acactcgcca tggtgccctc gatcagcttg agcaaggcct gctgaacacc ctcacccgag
     1141 acatcgcgag tgatggaggg gttatcggac ttgcgcgata ttttgtcgat ttcatcaata
     1201 taaacaatgc cgcgttgcgc ccgctccact tcgtaattgc aactttgcaa aagcttcaaa
     1261 acaatatttt cgacatcttc accaacataa ccagcttcgg tcagggtggt ggcatctgcc
     1321 atcacaaagg gcacatcaag catgcgggcc agcgtctgcg ccagcaaggt cttgcctgat
     1381 ccggttgggc cgatcagcaa gatgttgctc ttggacaact ccacgtcatc ttttgtggaa
     1441 tgctcctgat gccgcaaacg cttgtaatgg ttgtacaccg ccaccgccag aatgcgcttg
     1501 gctggctcct gcccaatgac gtaattgtca agattggcct tgatctcgga aggcgtgggc
     1561 aaatcgttgc tgccgacgct ttccgtcttg gcaggaagtt cgtcgcgaat gatctcattg
     1621 cacaggtcaa tgcactcgtc acagacaaac accgaggggc cggcaatcag ttttttgact
     1681 tcgtgctgac tcttgccaca aaacgagcaa taaagggtct tgtcacccga ggagcctttt
     1741 ttttcggcca tgagaactat gctctcttcg aaatgacttg gtcaatcaga ccatattcct
     1801 tggactcttc agccgacatg tagtagtcgc gctcggtgtc gcgctcaatc cgatccagcg
     1861 gttgaccggt gcgctcagcc agtatcttgt tgagttgctc gcgcgtcttc aaaatctcgc
     1921 gggcctgaat ttcgatctcg gtggcctgcc cttgactacc gcctgagggt tggtgaatca
     1981 tgattttcga gttgggcagc gaaaaccgct tgcccttggc acctgctgcc agcaaaaaag
     2041 cacccatgct ggctgccatg ccggtgcaca gggtggacac ttcaggtttg atgaagttca
     2101 tggtgtcaaa aattgccatg ccagagctga ccgatccgcc cggcgaattg atgtaaaaag
     2161 aaatgtcctt gtcaggattt tcactctcca gaaacagcaa ttgggccacc accaggttgg
     2221 ccacatgatc attgaccggg ccgaccatga aaatgacgcg ctccttgagc aagcgtgagt
     2281 aaatatcata agaacgctca ccccgacccg attgctcaat caccatgggc accatgccaa
     2341 ggccctgaac atctgttgca ccgccgtatg aattcatttt gactccaaaa aacttacgct
     2401 actgtagcta aaaaaacctg acctcaaagg aaatggggct tgtgtaccag accagtcgtc
     2461 cgacacaagc cccaccagga acccatgggc tccaaggaat aatcagttga ataatcagtt
     2521 ctgcgccatc agttcttcaa aggtgattgt cttttcagta actttggctt gggacagcac
     2581 aaaattggtg acgttatttt caagcacgat gccttcaact tcagccagct ttttgctgtc
     2641 actaaagtac cagcgggaaa cttcagccgg tttttcatag gtggcggcga gttcttcgac
     2701 gtacgccttg atctgctcca ttttgacttg cagatcattg gcgcgcacca gttcagccac
     2761 caccagaccc aggcgcaccc gccgctcggc ctgcggacga aacacatcat caggaatggc
     2821 gaccttgtcg gcatccttga tgccacgctt cttcaggtca gcccgggcac cttcaagcaa
     2881 gcggtccatc tcagcccgga cactggcgtt cggcaaatcc agttcagcct tggcaaccag
     2941 ggcatccatc acagcggtct tgttacgggc cagcaaacgg ttcttgacct cacggtccag
     3001 gtttttcctg atgtccacac gcaaactatc gaccgacgca ttggcaatgc ccagcgactt
     3061 ggccagcgcc tcgtccacgt cgggcaaatg ggctgtctct atctttttca cggtgaccat
     3121 gaaatcagcg gttttgccag cgacatcctt gccgtggtag tcgaccggaa acaccagggg
     3181 aaaggttttg ctctcaccga gcttgaggcc ctgggtggct tcttcaaact ccttgagcat
     3241 ctggccatcg cccaaaatga actgaaagtc ttccgccttg ccaccctcaa aggcttcgcc
     3301 atcaatcttt ccctcaaagt caaccagcac ccggtcgcca tcctgcgccg ctgcgtcatg
     3361 gggccgctgc gagaaggtgc gacgctgctt gcgcaggatg tccacggttt tttcaaccgc
     3421 agcatcggaa acttcggtcg ttaccttttc aacctcggcg gttgccaagt caccaatggt
     3481 gacctccggg aaaacctcaa aaatggcctc aaaagccaat tcaccctcag cagcgccctc
     3541 tttctcgctg atctgggggc gtccggccac gcgcagttgc gcttcgtggg ctgcgttaaa
     3601 aaaggcctcg cccacctttt cattcatgac ctcatgctgc acgccgtagc cataccgctg
     3661 tgccacgacg ttcattggaa ctttgcccgg tcgaaacccg tccatcttga ccgttcgtgc
     3721 cagctttttc aaacgcgatg ccacttcgga ctgaatgatg ccaatgggca aactcagcgt
     3781 cattttgcgc tccaactttt caagaatttc aacggcaaca ggcatgaata aatctccaaa
     3841 aatatatgtg gtgcgcgggg ccggggccaa ccccacaccg tactttgcta tcgccagtac
     3901 ctaaacttgg cccgccacac tcatacaaaa aaaatgattc ccagggtcag cccctgaaaa
     3961 gcaattaaaa aaaggactcg cgccccaaat ccatcgaact accaaacagg taaacacacg
     4021 tcctcactca aaatttgagt tgaagatcag tatgtttcgc gggattgagg tagccggact
     4081 gatagacgct tcatattgct tggcgatgtc aaccaagtgc cattttcgct tcaacctggg
     4141 agccctatcg agtcgcgtat tgtactcaac gaaaaagaag cgccactgat acgatgcccg
     4201 acagagggga aaagccgcgc tgtttcatga cagggttgtg acccaaaaag gccttttgcc
     4261 cccaactcaa ccattcttga cgggcatttt cgccaaattc aagctaggag gcgatcggac
     4321 ttctggcgta tcggcggcgc agcgacagga gcggccggta ttgaattggc cttttgacac
     4381 atcgccgggc aaacgatcgg caaatccggc ataaacctgc ctgactgggg tcaatacccg
     4441 ctactcgaat ctggctcacc gcccccgccg cacgcgcatc agctcatcca gaatcaggca
     4501 ggttaccccc agcgtgatgg cactgtcggc aacgttgaag gccgggaaat gcctgccggc
     4561 ccagtgaaag tccagaaaat caaccacatg gccgtgcagc acccggtcca ctacgttacc
     4621 gactgcccca cccaaaacac tggcgatggc aaaggcaaac agtttctgtc cggcgtgcaa
     4681 cttgagtaac catagcatca gcagtgcagc cactatgccg atggcggtaa agaaccagcg
     4741 ttgccagcca ccggcgctgg ccaaaaaaga aaaggctgcg ccactgttat gcacccgcac
     4801 cacattcaag aagccggtga ccattgtgct gtcgccaagc tggtagtagc ccatgatcag
     4861 caccttggtg aactggtcaa gcatcagcac cagcaaggcc caggccagcc agggcagcag
     4921 accagcagaa ctgcctgatt tgtagtttcg gctcagagtg gcttgcgcca ggttgggttt
     4981 acgtgccatc aaagatacca aatagtttcc tggcaagggg tttcacgcga attgacgcgc
     5041 ttcacccggg ccgaaaaggt tgctggtgca acggccacac agggttgggt gggtgttgtc
     5101 attgccgaca tcatcccggt aatgccaaca acgctcgcat tttgggtcaa ttgaggctct
     5161 agagcttatg ctgattgcgt caccagctat taattcaata gtagaagtaa tgaacacaaa
     5221 cttcaggtct gcgcccagac ttgccagcaa ggcatggtcg tctgcgccta ccgttaggct
     5281 gatgttggct tgcagcgacg agccgagttt gccatcggca cgcaacagtt cgatgtcctt
     5341 gttgaccgcc tcccgaattt cgcggatgcg cgcccatttg gccacaagct caggctgcct
     5401 ggccaagccg cttgactcat cggaaaacga aaaaaagcta tccatgaaaa tggactcgcg
     5461 cgtcgccgac ggcgttctgc cctgctgccc cagcaccgcc caggcttctt cggcggtaaa
     5521 actcaagaac ggtgccatcc agcgcagcat ggcttgcaga atctgccaca acgcggtttg
     5581 cgcactgcgc cgcgccagtg atttgggcgc ggtggtgtag agtcggtctt tcaaaatatc
     5641 gaggtagaac gagccgaggt cctcgctgca ataaatctgc aaccgagcta ccaccgggtg
     5701 aaattcatac acctcgtaat gcgccagaat gtcggcctgc agcgcggcgg cacgggccag
     5761 cgcgtagcgg tctatttcca gcatctggtc aagcggcacg gcatcttcgg ccgggtcaaa
     5821 gtcactcacg ttggccatca aaaacttgag cgtgttgcga atgcgacgat aggtgtccac
     5881 cacccgcgcg aggatcttgt cgtcgccggc aatgtcgccc gagtagtcgc tggcggccac
     5941 ccacaggcgg atgatttcgg caccaagctt tttcgatgtc tcttgcgggt caacgccgtt
     6001 gccggcgctt ttgctcatct tgtggccctt gctatcgacc gtgaagccgt gcgtcaggat
     6061 gcctttgtac ggtgcccggt catacatggc gcaggctgtc agcaggctgg agtggaacca
     6121 gccgcgatgc tggtcgtggc cttcaaggta caggtcggtc tccggtccgg gcttgccagt
     6181 gcgcgggtca atgtcgtcgt ggccactgcc ggggtgtgaa cctttgagca ccgttgtgtg
     6241 ggtggtgcca gagtcaaacc agacctccag gatgtcgctg ctcttggtgt agtggtcggc
     6301 atcagcagca cagtcaactt ttgccagaat ggcttgagta ccggccttgc tccaggcttc
     6361 gatgccgcct tcttgcacca tgtcggcggc aatgtccaga atctccatgg tgcgcgggtg
     6421 tagctcaccg ctgtccttgt gcagaaaaaa cggcagcggc acaccccaac tgcgctgccg
     6481 actgatgcac cagtccggcc tgccggcgat catgtcgcgc aacctgatct tgccgttctc
     6541 cgggtaaaag gcggtgtttt caattgcatc gagcgccgtc tgacgcaggg ttttgggcgc
     6601 tttgtccttg gtaaaaacac cttcgcccgc gtccatgcgg ataaaccact gggcagcggc
     6661 gcggtagatc accggggttt tgtgacgcca gcagtgcggg tagctgtgga cgatgttctc
     6721 ggtggcaaac agccgcccgg cctcacgcag c
//
LOCUS       DAOSLL010000196         6739 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10278_length_6739_cov_4.987732, whole genome shotgun sequence.
ACCESSION   DAOSLL010000196 DAOSLL010000000
VERSION     DAOSLL010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6739)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6739)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6739
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10278_length_6739_cov_4.987732"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(275..1594)
                     /locus_tag="PLB25_09920"
     CDS             complement(275..1594)
                     /locus_tag="PLB25_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HOE41930.1"
                     /translation="MAMMFANVLTEVVRTVGLSAVMRAAGVVVRLLPIPQPTMMVGPG
                     SSARLGQAINDFGHNRVLVVTDPVVSKLGLMAPLLQALKAGGTEFVVFDQITPDAPIA
                     LIEKGVAVFQAQACDAIVAFGGGSAIDAAKTIGLAAANHKHPAQLVGYFKGRRAPAPI
                     YAVPTTAGTGSEVTVAAVISDPETNRKLVIADTRIVPRMAALDPTLMVGLPPDITAAT
                     GMDALTHAIEAYVGHWGTALTDRLALSAVGMIFESLPVAHVHGHDLAAREKMALASNY
                     AGQAFTRANVGYVHAIAHQLGGLYHTPHGLANAIMLPQVLRFLSPAIQPKLAVLALRA
                     KVGKKTDDEAVLAQKFLNAIDAMNTRLGIPATLDALRDEDVPALARAACREADANYPV
                     PLYMTQAICEAVIRQVLPTQAQPKAVAPKRPVRRIARGSKAAKIAIV"
     gene            1730..3859
                     /locus_tag="PLB25_09925"
     CDS             1730..3859
                     /locus_tag="PLB25_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HOE41931.1"
                     /translation="MKTVIGISLGASAHDFEFNISFLGEAMRVVRLGTDGSLAKAEKL
                     VKHWDKRATAIGLGVLRDSYKAASHRFIEKDSAKLKAVATHCPVTMGGQLGDILQEWA
                     IRHAQVELVSFFNNKRVLFFSGMKSYKLAMSMAEYTDNLKFADPISQLGVPKLLTSLA
                     ALQLYVSGAHYVRDWTLPTALSSGPVKEWTRFLLRKAMQKASVIVAPAHELDEFGLEE
                     LAGKTIITSTVSDERMALLATKGVHMVIDGAPVMQGHTLGPDLLDAMICAATGKGPSE
                     ILEDDYLEIITSLQLAPRIVYPNGFKRVNRFAFVIHPLSQEYFKKIKPIELLSQVSPP
                     VLMNSLEKIMAYAPPFVYSHVSGIQSPTGVEVEGWLISVGGTPREIMRHSPEFTYRRL
                     LDAAAMAKRLGAQIMGLGAFTKVVGDAGVTVAKRSPLPITTGNSYSASGALWAAHDAM
                     QRMRLVSIPKGQQRASFKGMVVGATGAIGSVCARLIAKVAEEVYLVSPETAKLLSLKE
                     SILLETPDAKLFLSATADKDIADMDMIVTATSGAGKKVLDIMQVKPGCVITDVARPLD
                     LPASEVAKRPDVLVIESGEIQLPGEHVLMKNIGLPKGVAYACLAETIVLALEGRFENF
                     TVGRAIEWEKVREIYKLGLKHGMKLAAISGVNGPFSDEDIERVRELALIARAEKAGPA
                     PKAAAKKASSVKKPAPARKAKSVPTPV"
     gene            4062..5342
                     /locus_tag="PLB25_09930"
     CDS             4062..5342
                     /locus_tag="PLB25_09930"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE41932.1"
                     /translation="MTLPSSLFSALFAHLPDSVFLIDPETSRIIDCNRTALQQLGMER
                     TEVLDQSVLSLQKDVVGIEQWGSIAQAIRATNCFVFVGQHRHKSGSEVPVEVNTSHFT
                     HGGNEYFLSVARNISQRLALERDLHNRDQQLRYALTEASDGLWDWNLQTDEVFFSPQL
                     ARMLGYGPHEMKPTLATWSANIHPDDAQRVQRVLQEHIEGQRERFLAEYRLRNRNGHD
                     LWVHDRGRVCERDPQGKPSRMVGMVTNITDQKAAELELQKLASHDPLTGLMNRRECDK
                     LLPRQLDLCRRLKLSLGLCIFDLDHFKEVNDQFGHAVGDLVLKRVAEVIAGQVRSTDY
                     LFRWGGEEFLLLCTDIDLPDLLTLVEKLRLLLGAIDWRDLVALPSITASFGVASFPEH
                     ANDAQDLFIAVDAVLYRAKAHGRNRVVQAPPKLV"
     gene            complement(5423..5974)
                     /locus_tag="PLB25_09935"
     CDS             complement(5423..5974)
                     /locus_tag="PLB25_09935"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOE41933.1"
                     /translation="MSHHAIGLVEFTSIAQGIAAADAMAKTANVEILVAKSICPGKYI
                     VMIGGDVAAVNQSVGTGTALCPDVVVDSFVIPNVHPSVLPAISGGTHIDNVRAISVIE
                     TYSVAATIEATDAAVKAASVEPIRMHLAFGIGGKSYVVLTGEVADIMAATDAGSAIAS
                     DKGFLVSKVVIPRPSRQLIDQLL"
     gene            complement(5984..>6739)
                     /locus_tag="PLB25_09940"
     CDS             complement(5984..>6739)
                     /locus_tag="PLB25_09940"
                     /inference="COORDINATES: protein motif:HMM:NF024768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOE41934.1"
                     /translation="RAGSITTNKPYFVNGGPMMGKLLDSIDQPVTKTTGGIIVLPEDH
                     IVIRRHRQPMQNIMATARAVCEQCNLCTELCPRHLIGHELHPALIVRSANYRDIGKPS
                     VLLSALTCSECAICEQWACPVDISPMRINIALKEKFRAEGARYVGELRPLDPMAEHRL
                     VPTSRLVTRLGIAPYNKKAPLDETETHPKAVVLNLRQHIGAAAVPLVRVGDAVQRGQL
                     LADIPPDALGARLHASISGTVSAVSSQSITLTA"
BASE COUNT         1328 a         2009 c         2058 g         1344 t
ORIGIN      
        1 gcatcccaac tccttggtgc aaaacgggga gttattcccg accacatcct aagtcgttga
       61 tttaccgtca ttcccgcgca ggcgggaatg acgaagttgc cataagaaca aatttaccct
      121 gcgggaatta gcggtttctc agtcctgacc cggttgacat gctgatcggc acacagttgg
      181 ctgcaaaaaa atgttcagcc cagcaccgct cagagtcttt aaaaatttga tttttttgcc
      241 ctgtaggcct tgtcaaataa gcgttaccag ctatttaaac aatagctatt ttggctgctt
      301 ttgagcctcg cgcaattcgc ctgaccggcc tcttgggtgc aaccgctttc ggctgggctt
      361 gggtcggcaa cacctgccga atcaccgctt cgcaaatcgc ctgggtcatg tacagtggca
      421 ccgggtaatt ggcatcggcc tcgcggcagg cggcacgcgc cagcgccggc acgtcctcgt
      481 cgcgcagcgc gtcaagcgtg gccggaatgc ccagccgggt gttcatggca tcgatggcgt
      541 tgagaaactt ttgcgccagc accgcctcat cgtcggtctt cttgccgacc ttggcgcgca
      601 gtgccagcac cgccagcttg ggttgaatcg ccgggctcag aaagcgcagc acctgcggca
      661 gcatgatggc attggccagg ccatgggggg tgtggtagag cccgcccaac tggtgcgcaa
      721 tggcatgcac atagccgaca ttggccctgg tgaaagcctg ccccgcgtag ttggatgcca
      781 gcgccatttt ttcgcgcgct gccaggtcgt gaccatgcac atgggctacc ggcaggctct
      841 caaaaatcat gccaacggcc gacaaggcca ggcggtcggt caacgccgtg ccccaatggc
      901 cgacgtaagc ctcaatcgcg tgggtcagtg cgtccatgcc ggtcgcagcg gtgatgtcgg
      961 gtggcaggcc gaccatcagg gtcgggtcaa gcgcggccat acgcggcacg atacgggtgt
     1021 cggcaatcac cagcttgcga ttcgtctcgg ggtcagagat gactgctgcc accgtgactt
     1081 cagagccggt gccagcggtg gtgggtactg cgtaaatcgg ggccggtgcc cgccgcccct
     1141 tgaaatagcc caccaattgc gccgggtgct tgtgattggc agcggccaag ccaatggttt
     1201 tggccgcgtc gatggccgag ccgccgccaa aggccacgat ggcatcacag gcctgcgcct
     1261 gaaataccgc gacacccttt tcgatcagcg caattggcgc gtccggggtg atctggtcaa
     1321 acaccacaaa ctcggtgcca ccggccttga gcgcctgcag caagggggcc atcaaaccca
     1381 gttttgatac taccgggtcg gtcacgacca gcaccctgtt gtggccaaag tcattgatgg
     1441 cctggcccag gcgcgcgctc gaccccggcc ccaccatcat ggtcggctgt ggaatgggca
     1501 gcagccgcac caccacgccg gccgcccgca tcaccgccga cagtcccacc gtgcgcacca
     1561 cttcagtcaa aacattcgca aacatcatcg ccatcctttt taaaatccag cttgcatcag
     1621 gcctgtgttg gcaggcatga tgcaccgtgc gaacgcattc ttgtagttgc atctctccaa
     1681 atcagtccac tcatcaggtt ccataatcag tccacttatc aggttccata tgaagacagt
     1741 tatcggtatc agcctggggg caagcgccca tgattttgaa ttcaacatca gctttctggg
     1801 tgaagccatg cgggtggtgc gcctgggcac tgacggcagc ctggccaaag ctgaaaagct
     1861 ggtcaagcac tgggacaaac gggccaccgc catcgggctg ggtgtgctgc gggacagtta
     1921 caaggcggcc agccatcgct tcatcgaaaa ggacagcgcc aaactcaagg cggtcgccac
     1981 ccactgcccg gtgaccatgg gtggccagct tggcgacatc ttgcaggagt gggcgattcg
     2041 ccatgcgcag gtggaactcg tcagcttttt caacaacaag cgggtgctgt tcttttcggg
     2101 catgaaaagc tacaagctag ccatgtcgat ggccgagtac accgacaacc tgaagtttgc
     2161 cgacccgatc agccaactgg gcgtgcccaa gctgctgact tcgctggcgg cgttgcagct
     2221 ttatgtcagc ggtgcccatt acgtccggga ctggacgctg ccgaccgcac tctcatcagg
     2281 cccggtaaag gaatggacgc gtttcctgct gcgcaaagcc atgcaaaagg ccagcgtgat
     2341 cgtggcacca gcgcacgagc ttgacgaatt tggcctcgaa gagctggctg gcaagaccat
     2401 catcacctcc accgtcagtg atgagcggat ggcgctgctg gcgaccaagg gcgtgcacat
     2461 ggtgattgac ggtgcaccgg tgatgcaggg ccacacgctg gggccagacc tgctcgatgc
     2521 catgatctgt gccgccaccg gcaagggccc cagcgagatt ctggaagacg actacctgga
     2581 gatcatcacc agtctgcaac tcgcaccgcg cattgtttac cccaacggct tcaagcgcgt
     2641 caatcgcttt gcctttgtga ttcacccgct gtcgcaggag tatttcaaga agatcaagcc
     2701 aattgagctg ctgtcgcagg tgtcgccgcc ggtgttgatg aactcgctgg aaaaaatcat
     2761 ggcctatgcg ccgccattcg tttattcgca tgtcagcggc attcagtcgc caaccggggt
     2821 cgaagtcgaa ggctggctga tctcggtcgg cggcacgccc agggagatca tgcgccacag
     2881 ccccgaattc acctaccgcc gcctgctcga tgcggccgcc atggccaagc ggctgggggc
     2941 ccagatcatg ggcctggggg cgttcaccaa ggtggtcggc gatgccggcg tgacggtagc
     3001 caagcgctcg ccgctgccaa ttaccaccgg caacagctac agcgcctcag gcgcgctgtg
     3061 ggccgcgcat gacgcgatgc agcgcatgcg gctggtgtcc atacccaagg gccagcagcg
     3121 cgccagcttc aagggcatgg tggtcggggc caccggtgcc attggttcgg tttgcgcccg
     3181 gctgattgcc aaggtggccg aagaggtcta tctggtgtcg cccgaaaccg ccaagctgct
     3241 gtcgctgaaa gagtcgatcc tgctcgaaac gcccgatgcc aaattgtttt tgtcggccac
     3301 ggcagacaag gacattgcgg acatggacat gatcgtcacc gccacctcgg gcgctggcaa
     3361 gaaggtgctt gacatcatgc aggtcaagcc cggctgcgtc atcaccgacg tggcccggcc
     3421 gcttgacctg ccagcgtctg aagtggccaa gcgccccgat gtgctggtca ttgagtctgg
     3481 cgaaatccag ctccctggcg aacatgtgtt gatgaaaaac attggtctgc ccaagggcgt
     3541 ggcctatgcc tgtctggccg aaaccattgt gctggcgctg gaaggccgct ttgagaactt
     3601 caccgtgggc cgtgccatcg agtgggaaaa agtgcgcgaa atctacaagc tgggcctgaa
     3661 acacggcatg aagctggccg ccatcagcgg tgtcaacggg ccgttcagtg acgaagacat
     3721 cgaacgggtg cgcgaactgg cgctgattgc ccgtgccgaa aaggctggcc cggcgcccaa
     3781 ggctgcagcg aaaaaggcca gcagcgtcaa aaaacctgcg ccagcccgga aagccaaatc
     3841 ggtgccgaca cctgtgtaac tgcagatctg ctggttcgat tggaattttg actggcaccg
     3901 ctgtatggag caggtgtttg tgcgcccgac ttcggttggt cggcattggg tgaacaacat
     3961 gccgcaaatg tgtctgctgc gggtctgatg taaacgagct tcgactgacg cgttgcccgc
     4021 acggtcacaa tgctgtccac cgcttacagc cagcattcac catgacactg cccagttcgc
     4081 ttttttcggc cttgttcgcc catttgccgg acagtgtctt tctgattgat cccgagactt
     4141 cccgcatcat cgactgcaac cggacggcgc tgcagcagct tggcatggaa cggactgaag
     4201 tgctggacca gtcggtgctc agcttgcaaa aggacgtggt cggcatcgag cagtggggca
     4261 gcattgcaca ggcaatacgg gcgaccaact gttttgtttt tgtcggtcag caccgtcaca
     4321 aaagcggctc ggaggtgccg gttgaagtca acaccagcca tttcacccac ggagggaacg
     4381 agtacttttt gtcggtggcc cgcaacatct cgcaacgcct ggcgcttgag cgcgacctgc
     4441 acaaccgcga ccagcagttg cgttatgccc tgaccgaagc atccgacggt ttgtgggact
     4501 ggaacctgca gaccgatgag gtgtttttca gcccacaact ggcgcgcatg ctgggctatg
     4561 ggccgcatga aatgaaaccc acgctggcca cctggtctgc caacattcac cctgacgatg
     4621 cgcagcgggt gcagcgggtg ctgcaggagc atatagaagg acagcgtgaa cgctttctgg
     4681 ccgaataccg cctgcgcaac cgcaacgggc acgacctgtg ggtgcatgac cggggccgcg
     4741 tgtgtgagcg tgacccccag ggcaagccga gccggatggt cggcatggtg accaacatca
     4801 ccgaccagaa agctgccgag ctggagttgc agaaattggc ctcgcacgac ccgctgaccg
     4861 gcctgatgaa ccggcgcgag tgcgacaagt tgctgccccg ccaattggac ttgtgccgtc
     4921 ggctcaagct gtcgctaggt ttgtgcattt ttgatttgga tcatttcaaa gaagtcaacg
     4981 accaatttgg tcacgctgtg ggtgacctgg tgttgaaacg cgtggccgag gtgatcgcgg
     5041 gccaggtccg ctcaaccgac tatttgttcc gttggggcgg cgaagaattt ttgttgctat
     5101 gtaccgacat cgacctgcct gatttgctca ccctggtgga aaaactgcgt ctgctgctgg
     5161 gcgccattga ctggcgggac ctggttgcgc tgccatcaat taccgccagc tttggcgtgg
     5221 ccagctttcc tgagcatgcg aacgatgcgc aagacctgtt catcgccgtc gatgcggtcc
     5281 tgtaccgggc caaggcgcat ggtcgcaacc gggtggtaca ggcaccaccc aagctggtgt
     5341 gacagtggcg actgccatca cgtccgcaag actggtgctg cctgccaaac ggctcgtggc
     5401 aacgccaaca gttgcccgtt cttcaaagaa gctggtcaat caactggcgg cttgggcgcg
     5461 ggatcaccac cttggacacc agaaaaccct tgtcgctggc aatcgccgac ccggcatcgg
     5521 tggctgccat gatgtcggcc acttcgccgg tcagcaccac atagcttttg ccaccaatgc
     5581 caaaggccag atgcatgcgg attggctcga ccgaggctgc cttgaccgcc gcgtcagtgg
     5641 cctcaatggt ggccgcgacc gagtaggtct cgatcacgct gatggcgcgc acgttgtcga
     5701 tgtgggtgcc gccgctgatg gccggcaaca ccgacggatg cacgttgggg atgacaaagc
     5761 tgtccaccac cacatccggg cacagcgcgg tgccggtgcc gaccgactgg ttcacggcag
     5821 ccacatcgcc gccgatcatc acgatgtatt tgcctgggca aatgcttttg gcaaccagaa
     5881 tttcgacatt ggcggtcttg gccatggcat ctgctgcggc aataccctgg gcgatgctgg
     5941 tgaattcaac cagtccgatg gcgtggtggc tcatggtttt ctttcaggcg gtaagggtga
     6001 tggactggct tgagacggca ctgacagtgc cgctgatgct ggcgtgcagg cgggctccga
     6061 gcgcgtcggg cggtatgtcg gccagcagtt ggccacgctg cacggcatca cccacccgca
     6121 ccagtggcac ggcggcagcg ccaatgtgct ggcgcaggtt cagcaccaca gctttcggat
     6181 gggtttcggt ctcgtcaagc ggggcctttt tgttgtaagg cgcaatgccc agccgtgtca
     6241 ccaggcgcga agtgggcacc agccggtgtt cggccatcgg gtcaagtggc cgcaactcac
     6301 ccacatagcg cgcgccttca gcgcggaatt tttcctttag cgcgatattg atgcgcatcg
     6361 gcgaaatgtc caccgggcaa gcccattgtt cgcaaatcgc gcactccgag caggtcagcg
     6421 ccgacagcag caccgaaggc ttgccgatgt cgcggtagtt ggcgctgcgc acaatcagcg
     6481 ccgggtgcag ttcgtggccg atcaggtggc gcgggcacag ttcggtacac aggttgcatt
     6541 gctcacacac ggcgcgggcg gtggccatga tgttttgcat cggttgccgg tggcggcgaa
     6601 tcacgatgtg gtcttcaggt agcacgatga tgccgccggt ggttttggtg accggctggt
     6661 cgatgctgtc gagcaacttg cccatcatcg ggccgccatt gacgaaatac ggcttgttgg
     6721 tggtgatgct gcccgcgcg
//
LOCUS       DAOSLL010000197         6710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10342_length_6710_cov_4.444177, whole genome shotgun sequence.
ACCESSION   DAOSLL010000197 DAOSLL010000000
VERSION     DAOSLL010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10342_length_6710_cov_4.444177"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1527)
                     /locus_tag="PLB25_09945"
     CDS             complement(<1..1527)
                     /locus_tag="PLB25_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HOE41935.1"
                     /translation="MLQRTSFIGSGPVLLGLLAGSALQLQQPLLWPTWVYACFVPLAL
                     ALYALTAMKTKADRDTARPTQSLLLALLVALLAFASTGWRASTYASAGLDPSVEGRDV
                     ALIGLVAGLPQRSEAGLRFRFVPESARLDGQPLRLPAQLELTWYGGVFGQGDGPGEMV
                     GELQRLPAPLQAGERWQLTARLKAVHGSVNPHGFDFELWQWEQGVQATGYVRAGAKDP
                     LPQRLTSTWRYPVDRARQSVRERIFQRVGDASAAGLIAALVVGDQGAIERLDWDVFRA
                     TGVAHLMSISGLHVTMFAWGAGLLVGWLWRRSSSLCLRLPAPSAALSGGVVLALAYAV
                     FSGFGVPAQRTVLMLATVALLRLSGLRWPWPQVWLLTCAVVVVADPWALLQAGFWLSF
                     VAVGVLFASDSGAGSADGKRATGQFVANIRQQWVITLALTPLSLVLFGQVSLVGLPAN
                     ALAIPWVTLVITPLAMLGVLVPHLWDAAAWACWLMVELLRWLAALPGAVWSVAQAPGW
                     A"
     gene            complement(1543..4917)
                     /locus_tag="PLB25_09950"
     CDS             complement(1543..4917)
                     /locus_tag="PLB25_09950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE41936.1"
                     /translation="MNLPGRAVLPLRLLLVRQVGVLMAGTVLLVGACFVVFGMLPMAD
                     QIARDQFDSAGARVEGGLSAVFTPPARLLAVSHGWLAGQAPDLASPQAFNASFKPLLQ
                     EFSQLTSVVAGTSTGQGWLLLQQPGGAWRNRMTDVARWGTQRHLLIEHDAIGRVSQRW
                     SEQIYDPRTRPWFQGAMSTPGELAVFWTAPYTFFTTGDPGITVSARSKLAGGADFVLG
                     FDLTLRDLSKVTMGASVGMTGLALVVTDDERVLALPGAPAGVSAAEWFGRMLKPVTAL
                     GLPAASAALARWHELNRPEGAVLAYQSGGTAWLASVRPYVLGTQRLWVLVLAPKADFA
                     LAWRPIALALGLALLLVLLLASWLLRTAALRLSEPLEALALASRRIGQLDFQGVVQPP
                     SQIAEIVALTSSQQGMLDTLRQNQQELDARAQALQQQVHALRRAETRLLEQNDQLSTI
                     IENFPGGVLVVDADLRVLAFNVRYQTMLALPDELMKKPGLRFGDLLRFNARRGDYGPV
                     DVEQLVAERLALVRQSLPQRSERVLASGSVIEVRRTPLPNGGFVSLYMDVTVSKKHQR
                     ELEHLAHFDALTGLPNRVLLADRLRHGMAQVARRGQRLAVAYLDLDGFKFVNDTYGHE
                     VGDQLLLLLSGRMKQALRDGDTLARIGGDEFVAVLMELAGPSESEPLLTRLLAAADAP
                     ASLLGLELRVSASIGVSFFPQNQEVDAEQLMRQADQAMYQAKQAGKNRYQFFDAEHDL
                     SLPGQIESLQRVRLALTGSEFVLHYQPMVNLRSAAVVGAEALIRWQHPQQGLLLPGAF
                     LPLLEEHALAIELGRWVIDTALAQMASWLAAGTHLPVSVNVGARQLQHSGFVEDLRAS
                     LVRHPQVNPADLQLEVQEISALEDLARVTEVMAQCRAMGVHFALDDFGTGYSSLTYLK
                     RLPVSQFKIDQSFVRDMLDDPDDLSILAGVVNVSVSFHRAVIAEGVESVAHGQLLLRL
                     GCDLAQGFGIARPMLAGELAGWVANWQGDPLWGDVPVVSRHDQLLLFAGAEHRAWVLG
                     LEAYLKGERQAALQHDRGDCNIGRWLADANKLSHPPGPAVVQNISLLHQQMHDLGTHL
                     CQLKDQNQSQVALQLLPTMRQLRDELLAQIEALLSP"
     gene            complement(5124..5381)
                     /locus_tag="PLB25_09955"
     CDS             complement(5124..5381)
                     /locus_tag="PLB25_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41937.1"
                     /translation="MNQDVSVGSARARLGQGIRAGRKLRLQTCLRKFRRLGQASLVAW
                     LRPAWRSKLRGRDDDDALTCWHVVLADEGSATNPGAASLNF"
     gene            complement(5378..6217)
                     /locus_tag="PLB25_09960"
     CDS             complement(5378..6217)
                     /locus_tag="PLB25_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOE41938.1"
                     /translation="MDPAALLSPRFVIPSAPRLVALLLAELARPEPDLRRVDQFISTD
                     PALTTRVLQAANASFFKLVGQIHCVSEALAVLRLSQVQTMVTSAATLTSSRSVPGLAL
                     PQFWAYSRDCARVARSLAGLVRQSQQAAFTAGLIHAVGMLAMRSAMVQAVVLDAEAGP
                     LDLKRERLERAAFGFGYTQASAWLAQQWHFPTVIVDALQNQFAPFENDGHEPLAGVIH
                     LATWRSRARHAGLTENMMTVTFPAAVAGMLGIDIDMVLQQDPIDWSRQVPGRALIEPA
                     RSS"
     gene            complement(6226..>6710)
                     /locus_tag="PLB25_09965"
     CDS             complement(6226..>6710)
                     /locus_tag="PLB25_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HOE41939.1"
                     /translation="DPRPDFRVARLHDGVEDIRDLTEGMLLEGTVSNVAAFGAFIDLG
                     VHQDGLVHVSQLSHKFVTDAREVVKTGDIVKVQVIEVDVARKRISLTMKLGAPPARRD
                     GSGQGTSDNRFQGANAQQRARPIGQNSGALAGPGRAAGNIAPQSTAMSSAFAKLKGLG
                     "
BASE COUNT         1347 a         2314 c         2016 g         1033 t
ORIGIN      
        1 agcccagccg ggggcttgcg ccactgacca gaccgcgccg ggcaaggccg ccagccagcg
       61 caacagctca accatcagcc agcaggccca ggcggcagcg tcccacaagt gcggcaccag
      121 cacccccagc atggccagcg gcgtgatcac cagcgtgacc cacggaatcg ccagcgcgtt
      181 ggcgggcaag ccgaccagcg aaacctggcc aaacagcacc agcgacaagg gggtgagcgc
      241 cagcgtgatc acccactgct gccgaatatt tgcgacaaat tggcctgtag cccttttgcc
      301 atctgcgctg ccagctcctg aatcagaagc aaacagaacc cctacagcaa caaagctcag
      361 ccagaaacca gcctgcagca aggcccacgg atcagccacc accaccactg cacaggtcag
      421 cagccaaacc tgcggccagg gccagcgcag gccagacagg cgcagcagcg ccaccgtggc
      481 cagcatcagc acggtgcgct gggctggcac gccaaagcca ctgaaaaccg cataagccag
      541 cgccagcacg acaccacccg acagggccgc gctgggtgcc ggcaagcgca ggcacaggct
      601 gcttgagcga cgccacagcc agcccaccag caaacccgcg ccccaggcga acatggtgac
      661 atgcaggccc gaaatgctca tcaggtgagc gaccccggtg gcccggaaca catcccagtc
      721 aagccgctcg atggcaccct ggtcgcccac caccagcgcc gcaatcaggc cagcagcgct
      781 ggcatcgcct acccgctgga aaatgcgctc gcgcacgctc tggcgtgccc gatcaaccgg
      841 atagcgccag gtgctggtca ggcgctgcgg cagcgggtct ttggcaccgg cacgcacata
      901 accggtggcc tgcacgcctt gttcccactg ccacaactca aagtcaaagc catgcgggtt
      961 gacgctgccg tgcaccgcct tgagccgcgc cgtcaactgc cagcgctcac cagcttgcag
     1021 cggtgctggc aggcgctgca attcgccgac catttcgccc ggcccgtcgc cctggccaaa
     1081 gacaccgcca taccaggtca gctcaagctg cgctggcaag cgcagtggct gtccgtccag
     1141 gcgcgccgat tccggcacaa aacgaaaccg caggccggcc tcagagcgct gcggcaagcc
     1201 cgccaccagg ccgatcaacg ccacatcgcg cccttccacc gacgggtcca ggccagcgct
     1261 ggcataggtg ctggcgcgcc agccagtgct ggcaaatgcc agcagcgcaa ccagcagcgc
     1321 cagcagcaac gactgggtgg gccttgcggt gtcccgatct gcttttgttt tcatagctgt
     1381 cagcgcatac agcgcaaggg ctaaaggcac aaaacatgca taaacccaag tgggccacaa
     1441 gagcggttgc tgcaattgca gggcgctgcc cgccagcagc cccagcagca cagggccgga
     1501 cccaatgaac gaggtgcgtt gcagcatgcg accagggcgg tattacggcg acagcaaggc
     1561 ctcaatttgc gccagcaact catcgcgcaa ctgccgcatg gtcggcagca gttgcagtgc
     1621 tacctgggac tggttctggt ctttgagttg gcacaggtgt gtgcccaggt catgcatctg
     1681 ctggtgcaac agcgagatgt tctgtaccac agccggtccg ggggggtgcg acagcttgtt
     1741 ggcatctgca agccacctgc cgatattgca atcaccgcgg tcatgttgca gcgcagcttg
     1801 ccgctcgcct ttgaggtagg cctccagccc aagcacccag gcgcggtgct cggcaccggc
     1861 aaacagcagc agttggtcgt gccgcgacac caccggcaca tcgccccaga gcgggtcgcc
     1921 ctgccagttg gcgacccagc ccgccaattc accggcaagc atcggccggg caatgccgaa
     1981 accctgagcc agatcacaac ccaggcgcaa cagcagttgc ccatgggcta ccgactcgac
     2041 cccttcggca atcaccgccc gatgaaacga taccgagacg ttgaccacac cggccagaat
     2101 cgacaaatcg tccgggtcat cgagcatgtc acggacaaaa ctctggtcga tcttgaattg
     2161 cgacaccggc aggcgcttga ggtaagtcag cgacgaataa ccggtgccaa aatcgtcaag
     2221 tgcaaagtgc acgcccatgg cccggcattg cgccatcacc tcggtgaccc gcgccaggtc
     2281 ttccagcgca ctgatctctt gcacctccag ttgcaaatca gccgggttga cctgcggatg
     2341 gcggaccagg ctggcgcgca agtcttcaac aaacccgctg tgctgcagtt gccgcgcacc
     2401 gacattgaca ctgaccggca ggtgtgtgcc cgccgccagc cagctcgcca tttgcgccag
     2461 ggcggtgtcg atcacccagc gaccaagctc aatcgccagt gcatgctctt ccagaagcgg
     2521 caggaaggcc ccgggcaaca acagcccctg ttgcgggtgc tgccagcgga tcagcgcctc
     2581 ggccccgacc accgccgcgc tgcgcaggtt gaccattggc tggtaatgca gcacaaattc
     2641 gctgcctgtc agcgccaggc gaacccgctg caaggactcg atctgaccgg gcaggctcag
     2701 gtcgtgctcg gcatcaaaaa actggtagcg gttcttgcca gcctgcttgg cctggtacat
     2761 ggcctggtcg gcctgacgca tcaactgctc ggcatcaacc tcctggttct gcggaaaaaa
     2821 gctcacgcca atgctggccg aaacccgcag ctccagcccc agcaggctgg ctggcgcatc
     2881 ggcggcggcc agcaggcggg tcagcaacgg ctcgctctcg gacggcccgg ccagttccat
     2941 cagtaccgcg acaaactcgt cgccaccgat tcgcgccagc gtgtcgccgt cacgcagggc
     3001 ttgtttcatg cgaccggaca gcagcagcag caactggtcg ccgacctcgt ggccataggt
     3061 gtcgttgaca aacttgaagc catcaaggtc aaggtaggcc accgccagcc gctggccacg
     3121 ccgggccacc tgggccatgc catggcgcag ccggtcggcc agcaacaccc ggttgggcaa
     3181 gccggtcagc gcgtcaaagt gggccaggtg ttccagttca cgctggtgct ttttgctcac
     3241 cgtcacgtcc atgtaaagcg agacaaaacc gccgttgggc agcggtgtgc gccgaacctc
     3301 gatcacgctg ccgctggcca gcacccgctc ggagcgctgc ggcagcgact ggcgcaccag
     3361 cgccagtcgc tcggccacca gttgctccac gtcgaccggc ccatagtcac cgcgacgtgc
     3421 attgaaacgc agcagatcgc caaaccgcag gcccggcttc ttcatcagct catcgggcag
     3481 cgccagcatg gtctggtagc ggacgttgaa ggccagcacg cgcaggtcgg catccaccac
     3541 cagaacaccg ccaggaaagt tttcgatgat ggtcgagagc tggtcgtttt gctccagcag
     3601 ccgggtctcg gcccggcgca aggcgtgcac ctgctgttgc agcgcctggg cgcgggcatc
     3661 aagctcctgc tggttttggc gcagggtgtc gagcatgcct tgctgactgc tggtcagcgc
     3721 cacaatctcg gcaatctggc tggggggctg caccaccccc tgaaaatcaa gctgaccgat
     3781 gcgtcggctg gcaagtgcca gggcttccag cggctctgac aggcgcaggg cagcggtacg
     3841 cagcagccaa ctggcaagca gcaacaccag cagcaaagcc agccccagcg ccagagcgat
     3901 cggtcgccag gccagcgcaa agtctgcctt gggtgccagc accagcaccc acaagcgttg
     3961 tgtgcccagc acataggggc gcacgctggc cagccaggcg gtgccaccag actggtatgc
     4021 cagcaccgcg ccctcgggtc gattcagctc gtgccagcgc gccagcgcgg cactggccgc
     4081 tggcaaacca agcgccgtca ctggcttgag catgcggcca aaccactcgg ctgccgacac
     4141 ccccgctggc gcacccggca acgccagcac ccgttcgtca tcggtgacca ccagcgccag
     4201 accggtcatg ccgacactgg cccccatggt gactttggag aggtcgcgca gggtcaggtc
     4261 aaagcccagc acaaagtccg ctccaccagc cagcttgctg cgggcagaca ctgtgatgcc
     4321 agggtcaccg gtggtaaaaa aagtgtaggg cgcagtccag aacaccgcca attcgcccgg
     4381 ggtgctcatg gccccctgaa accagggccg ggtgcgcggg tcatagatct gctcgctcca
     4441 gcgctggctg acgcggccga tggcatcgtg ctcaatcagc agatgccgct gcgtgcccca
     4501 gcgtgcgaca tcggtcatgc ggttgcgcca ggctccaccg ggctgttgca gcagcagcca
     4561 gccctggcct gtcgaggtac cggccacgac tgaggtcaac tgggaaaatt cctgcagcag
     4621 cggcttgaaa ctggcattga aggcctgcgg cgatgccaga tcaggggcct ggccagccag
     4681 ccagccgtga ctcacggcaa gcaggcgggc cggtggcgtg aataccgcgc tcagtccgcc
     4741 ctcgacccgg gcaccagcac tgtcaaattg atcccgggca atctggtcgg ccatcggcaa
     4801 catgccaaag acaacaaaac acgcccccac cagcagcacc gttccagcca tcagtacgcc
     4861 gacctggcgc accagcaaca agcgcagcgg caacaccgcg cggccaggca ggttcatcct
     4921 tgatacctcg gttgaccgct tgctttcatc tttaggtcct catgagcatt gacttgttgg
     4981 cctcattggg gttcacccat tgggtgacag cgccacgcac ccgattgaac cactgattcc
     5041 atttccaagt cattggtgtc cggccaccct ggcgcagacc ttggaaacgc aaggcaaggc
     5101 gagatgacaa cggcatggat gattcagaaa ttcaatgacg ccgcgcctgg gttggttgcg
     5161 ctgccctcgt cggcaaggac gacatgccag caggtaagcg catcgtcgtc gtcgcgcccg
     5221 cgcagcttgc tgcgccatgc agggcgcagc catgccacaa ggctggcctg ccccagccgt
     5281 cgaaacttgc gcaggcaggt ttgaagccgc agctttcgcc ctgcccggat gccctgcccg
     5341 aggcgcgccc gggcgcttcc gactgaaaca tcctggttca tgaagagcgt gccggctcga
     5401 tcagcgcacg ccccggcacc tgccttgacc agtcaatcgg gtcttgttgc agcaccatgt
     5461 caatgtcaat cccgagcatc ccggccacgg ctgccggaaa ggtcacggtc atcatgtttt
     5521 ccgtcaggcc ggcatggcgc gcccgggaac gccaggtcgc caggtggatg acaccagcca
     5581 acggctcatg cccgtcgttt tcaaacgggg caaactggtt ttgcagcgca tccacgatca
     5641 ccgtcggaaa gtgccactgc tgcgccagcc aggcgctggc ctgggtataa ccaaaaccaa
     5701 aggcagcacg ctcgagccgt tcgcgcttga gatccagcgg cccggcctcg gcatcgagca
     5761 ccaccgcttg caccatggcc gagcgcatcg ccagcatccc gaccgcatga atcagaccgg
     5821 cggtgaacgc ggcctgctgg ctttgccgca ccagcccggc cagcgagcgg gcgacgcgcg
     5881 cgcagtcgcg gctgtaggcc cagaactgtg gcaacgccag acccggcacc gagcggcttg
     5941 aggtcagcgt cgcagccgat gtgaccatcg tctgcacctg gctcagacgc agcaccgcca
     6001 gcgcctcgct gacgcagtgg atctggccga caagcttgaa aaaactggca ttggcggcct
     6061 gcagcacgcg cgtggtcagg gccggatcgg tgctgatgaa ctggtcaaca cgacgcaaat
     6121 caggctccgg gcgagccagt tcggccagca gcaaagccac cagtcgcggt gcactgggaa
     6181 tgacaaagcg cggcgaaagc agtgcggcgg ggtccatcag tctggctacc ccaagccctt
     6241 gagtttggca aatgcagatg acatggctgt tgattgtggc gcaatattgc ctgctgcccg
     6301 gcccggcccg gcaagcgccc ccgagttttg gccaattggg cgtgcgcgtt gttgcgcatt
     6361 ggccccctga aaccggttgt cgctggtgcc ttggccgctg ccatcgcgtc gggcgggtgg
     6421 cgcaccaagc ttcatggtca gagagattcg cttgcgcgcc acgtccacct cgatcacctg
     6481 caccttcaca atgtcgccgg ttttgaccac ctcgcgcgca tcggttacaa acttgtggct
     6541 gagttggctc acatgcacca gcccatcctg gtgcacgcca aggtcaatga aggcgccgaa
     6601 ggcggccaca ttgctcaccg taccctccag cagcatgccc tcggtcaggt cgcgaatgtc
     6661 ttccacgccg tcgtgcaagc gcgccacccg gaaatccggg cgcgggtcac
//
LOCUS       DAOSLL010000198         6710 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10343_length_6710_cov_4.399850, whole genome shotgun sequence.
ACCESSION   DAOSLL010000198 DAOSLL010000000
VERSION     DAOSLL010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6710
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10343_length_6710_cov_4.399850"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1763)
                     /locus_tag="PLB25_09970"
     CDS             complement(<1..1763)
                     /locus_tag="PLB25_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HOE41940.1"
                     /translation="MYPTLQRVFGYTEFRPGQQEVIGALLAGRSAVAIFPTGAGKSLC
                     YQLPALLLPHLTLVVSPLLALMHDQLVFMRRHRIAAASIDSTQSREQTQSVMAQARAG
                     TLKILMVSVERLKNERFRQFIASVPISLLVVDEAHCISEWGHNFRPDYLKLPQQQREL
                     GIAQTLLLTATATPAVVADMQAKFAIDSQDVVRTGFYRPNLHLTVEPVSGPDKLRRLV
                     QWLAGRPNQPGIVYVTLQQTAEDVARHLKQHGIDAAAYHAGLLPEVRVDLQQRFMAGQ
                     CTCIVATIAFGMGIDKSNIRHVVHFDLPKSIEGYSQEIGRAGRDGLPATCLVLANRDS
                     LQVLENFVYGDTPEPTGIDQVLGEILAVAPQQPWQVQLTGLSDRSNIRLLPLKTLLVQ
                     LELRAIIAAQLSYFAEYRFKYLIDPAQLIAKFSGERADFVMAIVQSSIRAPTWCTLDF
                     QTLYQRHQAEHSRVVRALDYFQEKGWIELEAKHVTDVYAVLQPAFDRAALGAELSAYF
                     AAKETSEVARIHAMLDFFASEQCLSQRLAAYFGDGQAPLHCGHCSVCQGQVAHLPAPA
                     PLPALDSHNFAALCDLLIEEMG"
     gene            1902..2155
                     /locus_tag="PLB25_09975"
                     /pseudo
     CDS             1902..2155
                     /locus_tag="PLB25_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018310970.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(2139..2519)
                     /locus_tag="PLB25_09980"
     CDS             complement(2139..2519)
                     /locus_tag="PLB25_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41941.1"
                     /translation="MPSTQAQWDGWLAKSAQLVADLAALDAAGKRDERNALIDANSAH
                     CGALKEWLLTLSTGKCWVSEVRELYSYYDVEHFRPTKVAKALDACLRDGYWWLAFDCM
                     NFRGCSNVGNRKKDGWFPPAQAAS"
     gene            3044..3793
                     /locus_tag="PLB25_09985"
     CDS             3044..3793
                     /locus_tag="PLB25_09985"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HOE41942.1"
                     /translation="MQILLVSDLHYTLPQLDWVVRVAPSFDLVVLAGDSLDISSAVSI
                     DAQSVVILRYLELLQAAGRVVVSSGNHDLTGPDASGEQSALWLQEARMSGVPSDGDSL
                     LLSDTLITICPWWDGPVGRAGLQDQLAADALRRPRRWIWVYHWPPMGSPTCWTGRRHY
                     GDADIGGWISEFQPDMVLTGHVHEPPFKPDGAWADRIGNTWVFNAGRQIGPVPAHIEI
                     DLALGSASWRSMMGAEALKLTDLARPARSVF"
     gene            complement(3796..4278)
                     /locus_tag="PLB25_09990"
     CDS             complement(3796..4278)
                     /locus_tag="PLB25_09990"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOE41943.1"
                     /translation="MKDMITLSAHLPEVRFATGDVVVQEGGTAGGIWVLVSGALQVRK
                     GDVLINGINLPGAMVGEVSTLLGTAYGATVLATAPSVLRHAIDGQAFLASHPDISRLV
                     AVGLAERLNFVTTYLADLKHQYGDAPGLRMVSEVLVQLAQRQGPLARPGSLRDPNPDY
                     "
     gene            4465..6021
                     /locus_tag="PLB25_09995"
     CDS             4465..6021
                     /locus_tag="PLB25_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HOE41944.1"
                     /translation="MILELTVFLLFCAAALGWVAHRFQFPYPIALVAGGAMLGLIPRL
                     PQFPFDPQFILILILPPILYQAALMTSWSDFKAHIRPIGLLSIGLVVATTLAVGAALK
                     LLVPEVPWAAAFVLGAIVSPPDAVAATAILSRLKIPRRVVTVLEGESLVNDASGLVIY
                     KFAVVAVLTGTFSLSEALVQFGGIAIGGILLGIGTAHLFIAIHRQLDDPFIEILISLL
                     VPYATYIVAESLHASGVLALVAAGLVRGRYAPEIVSASMRILGRPAWQLVVFMLNSLT
                     FMLIGLQMSSILDKLSLYSPVQLLTLGSTVSLVAIGVRFVWVYTAALLPGGWGKRNTA
                     NAEPPNARENFIISWCGMRGIVSIAAALSLPLVLPDGSAFPHRDLIVFMTFFVITVTL
                     VVQGLSLPVLIRKLKVGTTWNEHEEKARVHAAISAAALQALDLHVETHAPPRAWAESL
                     RAEITNRITLAVPEGENHWTHEASIMHLRHAVIQAERKELIRLWRDNQVSDEVMHRIE
                     EMLDHQEAHL"
BASE COUNT         1403 a         1999 c         1886 g         1422 t
ORIGIN      
        1 cccatctcct cgatcagcag gtcgcacagg gcggcaaagt tgtgcgagtc cagcgcaggt
       61 aaaggcgcag gcgctggcag gtgcgccacc tgaccctggc agaccgagca atgcccgcag
      121 tgcagcggtg cctggccatc accaaaatac gccgccagcc gctggctcag gcattgttcg
      181 ctggcaaaaa agtccagcat ggcatggatg cgcgccactt cgctggtctc cttggcggca
      241 aagtaggcgc tcagctccgc acctagcgct gcgcggtcaa aagctggttg cagcaccgca
      301 taaacatcgg tgacatgttt ggcttccagc tcaatccagc ctttttcctg aaaatagtcc
      361 agcgccctga cgacccggct gtgctcggcc tgatggcgtt ggtacagcgt ctgaaaatcc
      421 agcgtacacc aggtgggcgc ccgaatcgag ctttgcacaa tcgccatcac aaagtcggca
      481 cgctcgccgc tgaatttggc gattagttgc gccggatcga tcaggtactt gaaacggtac
      541 tcggcaaaat aggacaactg tgcggcaatg atggcgcgca actccagttg caccagcagg
      601 gttttgagtg gcagcaggcg gatgttgctg cggtcagaca agccggtgag ttgcacttgc
      661 cagggctgct gcggtgccac cgccagaatc tcgcccagca cctggtcaat gccagttggc
      721 tccggggtgt cgccatagac aaagttttcc agcacctgca aactgtcgcg attggccagc
      781 accaggcagg tggctggcaa accgtcacgc ccggcacgac caatctcctg gctgtaacct
      841 tcaatcgatt tgggcaggtc aaagtgcacc acatggcgga tattgctttt gtcaatgccc
      901 atgccaaagg caatggtggc aacgatacag gtgcactgcc cggccatgaa acgctgttgc
      961 aaatcgaccc gcacttcagg cagcaacccg gcgtggtagg ccgccgcatc gatgccatgc
     1021 tgcttcaggt gccgcgccac gtcttcggcg gtttgctgca gggtcacgta cacaatgccc
     1081 ggctggttcg gccgcccggc cagccattgc accagacggc gcaatttgtc cgggccgctg
     1141 accggttcaa ccgtcaggtg caggttgggc cggtaaaagc cggtgcgcac cacgtcctgg
     1201 ctgtcaatcg caaacttggc ctgcatgtcg gccaccacgg ccggggtggc ggtagcggtc
     1261 agcagcaggg tttgcgcaat gcccaactcg cgctgctgct gtggcagctt gaggtaatcg
     1321 gggcgaaagt tgtgccccca ttccgagatg cagtgggcct catcgaccac cagcagcgag
     1381 atcggcacgc tggcaatgaa ttggcgaaag cgctcgtttt tcaaccgctc gaccgaaacc
     1441 atcagaattt tgagggtgcc ggcacgcgcc tgcgccatca ccgactgggt ttgctcgcgg
     1501 ctttgcgtcg agtcgatgct ggccgctgcg atgcggtgcc gtcgcatgaa aaccagttgg
     1561 tcgtgcatca gggccagcag cggcgacacc accagggtca agtggggcag cagcaaggcc
     1621 ggcaattggt agcacagcga cttgcccgcg ccagtcggaa aaatcgccac cgccgagcgc
     1681 ccggccagca gcgcaccaat cacctcctgt tggccggggc gaaactcggt gtagccaaaa
     1741 acacgttgca gggttgggta catgggatct gttgctccag tcatgactcg gttgcaaatt
     1801 atcactcttt ttggcctcta gccctttatc cataagcgct agcagctatg aaaatgaaag
     1861 tattgatgtg cctgaactga tcatgttgct gatgtgccca tctggaacag cctgatgccg
     1921 accttggcct ggctgatgct gacaagatca agatcaaaac aggtttggtg atcgagctca
     1981 gaaaagccgt gcgcacccgt ggcctgactc agcaggatgc cgctaaacgc atcggcacac
     2041 ccagcccaaa gtatccgacg tgatacgcga tggtttcacc aacctgtcgg agcgcaaact
     2101 gatggactgt ccaacccggc ttggctatga cattgagatc agcttgcggc ctgcgcaggc
     2161 ggaaaccaac catccttttt acggttgccg acgttactgc acccgcgaaa attcatgcaa
     2221 tcgaaagcca gccaccaata gccatctcgc aagcaggcat ccagcgcctt cgccactttc
     2281 gtaggacgaa agtgttccac gtcgtagtac gagtacagct cacgtacctc tgatacccag
     2341 catttgccag tcgacagggt caaaagccat tccttcaagg caccgcagtg cgcgctgttg
     2401 gcatcaatca gtgcgttgcg ctcgtcgcgc ttccccgcag catccaaggc ggccagatca
     2461 gccaccagtt gggcagactt cgccaaccag ccgtcccatt gcgcttgcgt cgacggcatc
     2521 cagtttgcga tgttggtatc tgtgggaagc ttgtgttcta gatcgaacca gttcagtggc
     2581 tgtgctcttc acgcaaaatt tcgtcgatga tggaatcggc aatcgcatcc tgctcgactt
     2641 gctcttccgg cgtgagcgtt aacgccaagc ctatgccgcg ctatcgctgc tgtttaatga
     2701 gcccgaatcg gaagttgcga agtgctttgc agcaccctgt cgcttgcgtg gaacgcggcg
     2761 ccttgactcg cacctttggc ggtttccgct gacccagggc aacttctttc agggcacgga
     2821 gaacttgacc gatgtgctgg aagtggtttg ctacagcccc attggaatgc tgcaacgcct
     2881 cggcaaaggc accaagttat tgccaaaatg gacaatacgc acatcggatt tcacctgcgg
     2941 atcagttgtc ggcaaaggca taaataatca aggcgacagt caccggggcg gcaaaaacgc
     3001 gtctaagatt gaaccggaaa accctccatt gatccagacg ttcgtgcaaa ttctcctggt
     3061 ttctgacctt cattacacgc tgccacagct tgactgggtg gtgcgcgttg caccgtcttt
     3121 cgacttggtg gtgttggcgg gcgattcgct tgacatcagc tcggcagttt caatcgacgc
     3181 gcaatcggtg gtcatcctgc gttatttgga actgctgcaa gcagccggtc gggtggtggt
     3241 gagttcgggc aaccacgatc tcaccggacc tgatgccagt ggcgaacagt ccgccttgtg
     3301 gttacaggaa gcgcgtatgt ccggggttcc cagcgacggt gattcgcttc tgctaagcga
     3361 caccctgatc acaatttgtc cttggtggga tggtccggtc ggtcgggctg gactgcagga
     3421 tcaattggca gccgatgcgc ttcgtcggcc gcggcgttgg atctgggtct atcactggcc
     3481 accgatgggc tcgccaacct gctggaccgg acgacgtcat tacggcgatg ccgatatcgg
     3541 cggttggatc agcgagtttc agccagacat ggtgctgacc ggccacgtgc atgaaccccc
     3601 gtttaaacca gacggtgcct gggctgatcg catcggcaac acctgggtgt tcaacgcagg
     3661 tcgccaaatc ggaccggtgc ccgcgcatat cgaaatcgac ctggcgcttg gctcggcctc
     3721 atggcgctcc atgatgggtg ctgaggcgct gaaactgacg gacctggcgc ggcccgcccg
     3781 aagcgtcttt tagcttcaat agtcgggatt gggatcgcgc aacgaccccg gcctggccag
     3841 cggcccttgc cgttgggcaa gctggaccag aacttcagac accatgcgca gcccgggggc
     3901 atcaccgtac tggtgcttca ggtcagccaa gtaggtagtg acaaaattga gccgctctgc
     3961 cagtccaacg gccaccagcc tgctgatatc ggggtggctc gccagaaacg cctgaccgtc
     4021 tatggcatgg cgcagcacgc tgggggcggt ggccagcacg gtggcaccat aggccgttcc
     4081 cagcaaggtt gaaacttctc cgaccatggc accaggcagg ttgatgccgt taatgagtac
     4141 gtcgcccttg cgcacctgca atgccccact gaccagcacc cagatgccgc cagcagtgcc
     4201 gccttcctga acgacgacat cgccagtggc aaatcggact tcaggtagat gggcagagag
     4261 tgtgatcatg tccttcattg gcctccatgg taagcgctaa tccactgaaa aaacaccttt
     4321 tgacaacacc cgtcatgcat tcgtcaagtt tgcctggcat ttgcctggcc gtaccagatt
     4381 cctctggtga acttgtgcga tagttaggaa ttctcgcccg tccgggcggc ctgcaacttg
     4441 gcctgacacc cgaaacatct ctacatgatt ctcgaactaa cggtcttctt acttttttgt
     4501 gctgcggcgc tgggctgggt tgcccacagg tttcagtttc cctacccgat tgcgctggtg
     4561 gccgggggcg caatgctggg gttgattccg cgacttcccc agtttccttt tgacccgcag
     4621 ttcatcctga tcctgatcct gccaccgatt ctttaccagg ccgcgctaat gacatcatgg
     4681 tcagacttca aggcgcacat cagaccgatc ggcctgctct cgatcggtct ggtcgttgcc
     4741 acaacactgg cagtgggcgc tgcgctaaag ctgctggtgc ctgaagtccc atgggctgct
     4801 gcgtttgtgt taggagcgat tgtttcccca ccggatgcag tcgctgccac ggccatcctg
     4861 tcaagactga agatacctcg ccgggtcgtc actgtgctgg aaggtgaaag cctggtcaac
     4921 gatgcctccg gactggtcat ctacaagttt gccgtggtag ccgtgctcac cgggacattt
     4981 tccctgtccg aggccctggt gcaatttggc ggcatcgcaa ttggcggcat tttgctgggc
     5041 atcggcacgg cccatctgtt tatcgccatt caccggcaac ttgacgaccc attcatcgaa
     5101 atactgatct ctctgctggt tccctatgcg acctacatcg tcgccgagtc cttgcacgcg
     5161 tccggcgtac tggccctggt agcagcaggc ctggtacgcg ggcgatacgc gccggaaatc
     5221 gtctccgctt cgatgcgaat cctcggacgg cccgcttggc aactggtggt gttcatgctc
     5281 aacagcctga ccttcatgct gatagggctg caaatgtcgt cgatcctgga caaactgtcg
     5341 ctctattcgc ctgttcaact gctgacgctt ggcagcacgg tcagcctggt ggcgatcggc
     5401 gtgcgctttg tttgggttta caccgctgca ctgctgccgg gtggctgggg aaaaagaaat
     5461 acagccaatg cggagccacc taacgcccgt gaaaacttca tcatcagttg gtgcggcatg
     5521 cgcggaattg tgtcgattgc tgcggctctt tcgttgccac ttgtcctgcc ggatggatct
     5581 gccttcccgc accgtgacct gattgtcttc atgacttttt ttgtcatcac ggtcacgttg
     5641 gtagtgcaag gcctgagcct gccggtgttg attcgcaagc tgaaagttgg caccacgtgg
     5701 aacgagcatg aagaaaaagc gcgtgttcac gccgccatca gtgctgcagc cttacaggcc
     5761 ctggatctgc acgtcgagac ccacgcgcca ccgcgcgctt gggcagagtc gctgcgcgcg
     5821 gaaatcacca accgcattac cctggccgtg ccggaagggg aaaaccactg gacgcacgaa
     5881 gccagcatca tgcacctgcg ccatgcggtg attcaggcag aacgcaaaga gctgattcgg
     5941 ttgtggcgag ataaccaggt cagcgacgaa gtgatgcacc ggatcgagga gatgcttgat
     6001 caccaagaag cacatttgtg agcccccgct cagagtaaac gtgtcccata catcaagctg
     6061 tcatgacttt gacaaccaaa aaatgggcag gaacggcctc attcggaaaa tctttgcctc
     6121 gaagttttga ggcatctcca agggtgctag cgccgaccca attccgcaaa aacctggcgg
     6181 cagaaatcat ctgcaaacct ggctgcaagc gcaaagctgc cgcacctgtt cccgctggtc
     6241 agccaaatag caacacgccc aatcaaggga tcgcctcgcc atgcggatag gcaacccgca
     6301 gttgacctag ccaaatggca ggccaagcga acttcgagtc caaaaaacag tcgacgaact
     6361 tggacgactt ccgacatacc tctgaataac acagccattc cgatggaaat cactgatcac
     6421 gccactacac aattgaaatt cttgaaaaca acaacgttga attcatcggg aatcaaagtg
     6481 aaacggaccg ctgttcagcc gaatccactt gcacagggtc gacagccccg gcacccgtta
     6541 ccaagaacca ctggcgcaag gccatcccca tgacctgcac cgccggtcgc ccccttttgt
     6601 gaaccactgt ctcaatgtga atccaatgtg gtactcgcat cgcaatttcg tgtggctcgc
     6661 taactcgtcc tgagtctcct gacgtttgct tcacgcttca cgcgtacgca
//
LOCUS       DAOSLL010000199         6704 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10358_length_6704_cov_5.194616, whole genome shotgun sequence.
ACCESSION   DAOSLL010000199 DAOSLL010000000
VERSION     DAOSLL010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10358_length_6704_cov_5.194616"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            10..1110
                     /locus_tag="PLB25_10000"
     CDS             10..1110
                     /locus_tag="PLB25_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HOE41945.1"
                     /translation="MFDPVHAGTLALANRIVMAPLTRNRAPDAIPTPLMAQYYTQRAS
                     AGLLITEATAISQQGQGYSDVPGLYGSEQIDGWKQVTKSVHAAGGKIVVQLWHVGRVS
                     HTSLQPNGGKPVAPSAITAKTKTVLIRDGVPGFVETSEPRALQAYELPDIVHTYAAAA
                     RNAVETAGFDGVEIHAANGYLLDQFLKSGSNQRRDDYGGSIENRSRLLLEVVRAVTQA
                     IGAERTGIRLSPVTPANDAFDPDPQPLFDYLVRELAGFQLAYLHIIEGATGGARTLPD
                     RPFDYTGLRRAYRAAGGKGAWMVNNGLDKALAQAALADGADLVAFGRPFIANPDLVER
                     LRRNKPFNEGDRNTFYGGGAKGYTDYPKLGKA"
     gene            1301..2281
                     /locus_tag="PLB25_10005"
     CDS             1301..2281
                     /locus_tag="PLB25_10005"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE41946.1"
                     /translation="MNTSPNLWVQSAPQWRATQSAGVDQLAQSISGWDQVYDQTSPGQ
                     FHGALTELMLGQVQLFIESSSHALVQTCKTWAGATWFGIPVHNRQVVRVDGTRLAPDM
                     LALHSGQGDFQLTTPDDFGFLGLVIHTDYLCHYAALEHSELPDHFLVQRVLQVPDAHL
                     AQFRRWLCQLLMAQPMQFEALTPASQQHLFDEAISGLVQLLAHGTSGPRENVSAQHAR
                     RTLRRAREYLQAQTDRCVTVHELCVHLGASPRALQDCFRKYVGLSPKTYLKAVKLNQA
                     HRELRRADSSLASVSDVAVRYGFWHLSQFAADYRWLFGELPSETLRKRGR"
     gene            2422..2757
                     /gene="eutS"
                     /locus_tag="PLB25_10010"
     CDS             2422..2757
                     /gene="eutS"
                     /locus_tag="PLB25_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HOE41947.1"
                     /translation="MTKERIIQEFVPGKQVTLAHCIANPTTELCKKIGVPGAEAIGIM
                     TLTPGETAMIAGDIATKAANVEIGFLDRFSGALVICGSVASVDEALQQVINTLRRQMS
                     YTTCEISRT"
     gene            2754..3233
                     /locus_tag="PLB25_10015"
     CDS             2754..3233
                     /locus_tag="PLB25_10015"
                     /inference="COORDINATES: protein motif:HMM:NF022128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HOE41948.1"
                     /translation="MSAGLKVGRFMLIGEIGAGKTTLFNHLFGRNEEARKTQVMEFEG
                     SSGIDTPGEYFSHPRLYHALITMAADVERLVYVHPADVFDCRLPYGLLDVYADKTIDA
                     IVTKMDLPGADLARVTALLRHAGIAGQIFAVSGTDLASMEKVRHHLLAAEPCLTEET"
     gene            3230..3913
                     /locus_tag="PLB25_10020"
     CDS             3230..3913
                     /locus_tag="PLB25_10020"
                     /inference="COORDINATES: protein motif:HMM:NF018008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41949.1"
                     /translation="MSKKQLIVAADVRLVAKSADKRIHLHDPKAIVTAEARSLAKELG
                     VELSFDQSEPVCDPPPSPPVDQDAVRRVIEAQTHSPASEAVMAEVLRRIAQERAQATT
                     APGHAAGDAPELAAPLAQIRKITSLCPSPTTSSSAVNMSQLDLATLGLTSAAPCACGF
                     MGWSNNLFPFVRSSDEINLVLEGELQFHVGQVVISARAGDVMWVPKGAQGKIGTPASV
                     RYFYLSYPV"
     gene            3917..5071
                     /gene="pta"
                     /locus_tag="PLB25_10025"
     CDS             3917..5071
                     /gene="pta"
                     /locus_tag="PLB25_10025"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF007233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HOE41950.1"
                     /translation="MGSFKAPASMACHGGLDPLAMLPDNWMPEPVAPDQPASALRQVP
                     LARLVFPDAQDQRVIEAAGTLSRCGWARPTLLANPLTLREHCAQHRITFPALRVIDPA
                     NSPSIDAYADQLRQRRPGMTQESVRSKLADPLWFAAMMLVCGDADYCIAGNLSSTASV
                     LRAALKVIGLSEGNHTLSSMFFMLAPDRSHVFGFADCGVVPKPTVEQLADIAISTAGT
                     FFNVTGEQPRVAMLSFSTLGSARHASAELVAQATVLARTRRPDLLIDGELQFDAAFVP
                     AVAARKAPDSPVAGKANVFIFPSLSEGNIAYKIAERLGGYTALGPMLQGLQRPMHDLS
                     RGCSASDIVQVSLLAMKMAPRADARMPPSAPLATAIQPRHTGQQQRLVSV"
     gene            5111..5401
                     /gene="eutM"
                     /locus_tag="PLB25_10030"
     CDS             5111..5401
                     /gene="eutM"
                     /locus_tag="PLB25_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HOE41951.1"
                     /translation="MEALGMIETKGLVALIEASDAMVKAARVKLVGFQQIGGGLVTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEAIFPIKGDGKPLD"
     gene            5423..5707
                     /locus_tag="PLB25_10035"
     CDS             5423..5707
                     /locus_tag="PLB25_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HOE41952.1"
                     /translation="MQLAVVVGQVVSTVRCDGLGKDRLLLVDFINAQGQPSGLRHVAA
                     DSVGAGNGEWVLVVSGSSARKANDGDLPIDLSVIGIVDQVVVEDQVTYRK"
     gene            5723..>6704
                     /locus_tag="PLB25_10040"
     CDS             5723..>6704
                     /locus_tag="PLB25_10040"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF011927.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOE41953.1"
                     /translation="MNTGDADLERIVKAVLQAIDRGAGGNTAGSTAVAAPARAASAAL
                     PATPLPQLPMGVFASLDDAVAEADVAYRQMRSVAMRSRVVAAIRLAGEKHAQELAELA
                     VAETGMGRVADKFVKNVAQALHTPGVECLVPTVLSGDHGLTLMENAAWGVVASVTPST
                     NPAATLINNAISMIAAGNSVVFAPHPAAKQVSQRTVALLNQAVVAAGGPCNVITTVAQ
                     PNIATAQALFCYPGIHLLTVTGGEAVVEEARKHTDKRLIAAGAGNPPVVVDETANLEK
                     AARDIVWGASFDNNIICVDEKEVIVVASVADRLKAEMTKHKAVELTADQAR"
BASE COUNT         1287 a         2111 c         2094 g         1212 t
ORIGIN      
        1 gccagcaccc tgtttgatcc ggttcacgcc ggaacccttg cccttgccaa ccgcatcgtc
       61 atggcaccgt tgacgcgcaa ccgcgcgccc gatgccattc cgacaccctt gatggcccag
      121 tactacaccc agcgcgccag tgccgggctg ctgatcaccg aggccactgc gatcagccag
      181 caaggccagg gttattccga cgtgccgggc ctgtatggca gcgaacaaat tgacggctgg
      241 aaacaagtca ccaagagcgt tcacgccgct ggcggcaaga tcgtggtgca gctctggcat
      301 gtcgggcgcg tctcacacac cagcctgcag cccaacggcg gcaagccggt ggcaccttcg
      361 gccatcaccg ccaagaccaa aaccgtgctg atcagggacg gcgtgcccgg ttttgtcgaa
      421 acctctgagc cgcgtgcgct acaggcttat gagttacccg acatcgtgca tacctacgca
      481 gcagcggccc gcaatgcggt ggaaacggct ggttttgacg gggtggaaat ccacgctgcc
      541 aatggctatt tgcttgacca gttcctcaaa agcggctcga accagcgccg tgacgactat
      601 ggcggcagca tcgaaaaccg ctcccggctg ttgcttgaag tggtgcgcgc cgtcacccag
      661 gccatcggtg ccgagcgcac tggcatccgc ctgtcaccgg tgaccccggc caacgacgcg
      721 tttgaccccg atccgcagcc gctgtttgac tatctggtgc gcgaactggc tggcttccag
      781 cttgcctacc tgcacatcat cgaaggggcc accggcggtg cccgcacctt gccagaccga
      841 ccgtttgact acaccgggct gcgccgggct taccgcgctg ccgggggcaa aggagcctgg
      901 atggtcaaca acggcctgga caaggcgctg gcgcaggccg cattggccga cggtgccgac
      961 ctggtggcgt ttggccgccc cttcatcgcc aacccggacc tggtggagcg actgcgccgc
     1021 aacaagccct tcaatgaagg tgaccgcaac accttttacg gtggcggtgc caagggttac
     1081 accgactatc cgaagctggg caaagcctga gcaaagcggc taacggccga tgccatgcgc
     1141 cgaccaggca ttgcgaaagt cggcaatccg gtcagcttga cccaggcgaa ctctcttgac
     1201 atgccgagac tggccgcgaa aaatgcggct tggcacaatt gcggcacaaa aactgcttta
     1261 accggtcaaa ccaaccttcg cgcgaaacag gcatcgctct ttgaacacct cgccaaacct
     1321 ctgggtccag agcgcgccgc aatggcgggc cacgcagtca gcgggcgtgg accagttggc
     1381 gcagagcatt tcgggctggg accaggttta tgaccagacc tcgccgggtc agtttcacgg
     1441 tgccttgacc gagctgatgc tggggcaggt gcagttgttc atcgagtcgt caagccacgc
     1501 actggtacaa acttgcaaga cctgggccgg ggccacctgg tttggcattc cggtgcacaa
     1561 ccggcaggtg gtgcgggtcg atggcaccag gctggcgcct gacatgctgg cgctgcattc
     1621 gggccaaggc gacttccagt tgaccacgcc tgacgatttt ggctttttgg gactggtgat
     1681 ccacaccgac tacctatgcc actatgcggc ccttgaacac agtgaactgc ccgatcactt
     1741 tctggtgcag cgggtgctgc aggttcctga tgctcatctg gcgcaatttc gccgctggct
     1801 gtgccagttg ctgatggcac agccgatgca gttcgaggcg ctgacacccg cttcacagca
     1861 gcatcttttt gacgaagcca tttccggcct ggtgcagctc ctggcacacg gcaccagcgg
     1921 accccgcgaa aacgtgtcgg cgcagcatgc acggcgcacc ctgcgtcgtg cgcgcgagta
     1981 tctgcaggcg caaaccgacc gctgcgtcac ggtgcatgag ctgtgcgtcc accttggtgc
     2041 cagcccgcgg gcgctgcagg actgctttcg caagtacgtc ggcctgtcgc ccaaaaccta
     2101 cctcaaggcc gtcaagctca atcaggcgca ccgggaactg cgccgcgccg actcaagcct
     2161 ggccagcgtc agcgacgtgg cggtgcgcta cggcttctgg cacctgagcc agtttgctgc
     2221 cgactaccgc tggctgtttg gcgaactacc gtccgagacg ctgcgcaaac gcggtaggtg
     2281 aggctggcat cggcaaatca cgccgatttt tgctaacgcc tggcaccctg gtgttctcaa
     2341 aatgcggttc tgcgtgggtg tcgtgtttgc gcacccgcct tcacttaacc cgaatcaagc
     2401 ccgatggatg ccaagcaagt cgtgaccaag gaaagaatca tccaggaatt tgtgcctggc
     2461 aaacaggtca cgctggcgca ttgcattgcc aaccccacca ccgaactgtg caaaaaaatc
     2521 ggtgtgccag gtgccgaggc gattggcatc atgaccctga cccccggcga gacggcgatg
     2581 attgctggcg acatcgccac caaggcagcc aatgttgaaa tcggctttct ggaccggttc
     2641 agcggtgcct tggtgatttg cggctcggtg gcttcggtcg atgaggcgct gcagcaggtc
     2701 atcaacacct tgcgtcggca aatgagttac accacttgcg agatttcccg cacatgagcg
     2761 ctggcctgaa agtggggcgc ttcatgctga ttggcgagat tggcgcaggc aagacgacgc
     2821 tgttcaacca cctgtttggc aggaatgaag aggcgcgcaa aacccaggtg atggaatttg
     2881 aaggcagcag tggcattgac acgccgggcg aatacttcag ccacccgcgc ctgtaccacg
     2941 cactgatcac catggcggcc gacgtggagc gcctggtgta tgtgcatccg gccgatgtgt
     3001 ttgactgccg cctgccttac ggcctgctgg acgtgtatgc cgacaaaacc attgatgcca
     3061 tcgtcaccaa aatggacttg cccggtgctg acctggcgcg cgtgacggcc ttgctgcgcc
     3121 acgctggcat tgcgggtcag atctttgcgg tctctggcac cgacttggcc tcgatggaaa
     3181 aggtgcgcca ccacctgctg gcagcagagc cctgcttgac ggaggaaaca tgagcaaaaa
     3241 acagttgatc gttgccgccg atgtgcgcct ggtggccaaa agcgctgaca agcggattca
     3301 cctgcatgac cccaaggcca tcgtgaccgc cgaggcacgc agtctggcca aggaactggg
     3361 ggtggaactg agcttcgacc agtcagagcc agtctgtgac ccgccgccga gcccgccggt
     3421 tgatcaggac gcagtgcgcc gggtgatcga agcccagacc cacagcccgg ccagtgaagc
     3481 cgtgatggcc gaggtgctgc gccgcatcgc gcaagagcgt gctcaggcca ccacggcgcc
     3541 aggccatgcg gcaggcgatg cgcccgagct tgcggcaccg ctggcccaaa tccgcaagat
     3601 cacctcactg tgccccagcc cgacaaccag cagcagcgcg gtcaacatga gccagctcga
     3661 tctggcaacc ctggggctga ccagcgcagc accttgcgcc tgtggcttca tgggctggtc
     3721 gaacaacctg tttccctttg tccgcagcag cgacgagatc aacctggtgc tcgaaggtga
     3781 attgcagttt catgttggcc aggtcgtcat cagcgccagg gccggtgatg tgatgtgggt
     3841 tcccaagggc gcgcagggca agatcggcac accagcctcg gtgcgctatt tctacctgag
     3901 ttacccggtg tgacagatgg gcagttttaa agcgccagcc agcatggcct gtcatggcgg
     3961 acttgatccg ctagccatgc tgccagacaa ctggatgccc gagcctgtcg cgcctgacca
     4021 gcctgcatcg gccttgcgcc aggtgccgct ggcccggctg gttttcccgg atgcgcaaga
     4081 ccagcgcgtg atcgaggccg caggaacctt aagccgctgc ggctgggccc ggccaaccct
     4141 gctggccaac ccgctgacgc tgcgcgagca ctgcgcccag caccgaatca ccttccccgc
     4201 gctacgggtg attgacccgg ccaactcgcc aagcattgat gcctatgccg accaactgcg
     4261 ccagcgccgc cccggcatga ctcaggaaag cgttcgcagc aagcttgccg acccgctctg
     4321 gtttgccgcc atgatgctgg tctgcggtga tgccgactat tgcatcgccg gcaacctgtc
     4381 atctaccgcc tcggtgttgc gcgcagcact caaggtgatt ggtctgagtg agggtaacca
     4441 caccctgtcg tcgatgtttt tcatgctggc accagaccgc tcgcatgtgt ttggctttgc
     4501 cgactgcggc gtggtgccca agcccacggt tgagcaactg gccgacattg cgatcagcac
     4561 ggcaggcacc tttttcaacg tcaccgggga gcagccgcgc gtggcgatgc tgtcgttctc
     4621 cacgctgggc agcgccagac acgcgtcggc cgaactggtg gcgcaggcca cggtgcttgc
     4681 cagaacccgc cgccccgact tgctgattga cggcgagctg cagtttgacg cagcatttgt
     4741 gccagccgtg gcggcgcgca aggcacctga cagcccggtg gccggcaagg ccaatgtgtt
     4801 catctttccg tcgctgtctg aaggcaatat tgcctacaag attgccgagc ggctgggcgg
     4861 ctacaccgca ctggggccga tgctgcaggg cctgcagcgt ccgatgcatg atttgtcgcg
     4921 cggctgcagc gccagcgaca tcgttcaggt gagcttgctg gccatgaaga tggcccccag
     4981 ggcggatgcc cggatgccgc cctcagcgcc gctggccaca gccattcaac cccggcacac
     5041 aggccagcag caacggcttg tgtcagtctg atttttcgtt ctacccacct cactaacctg
     5101 gagaaagcaa atggaagcac ttggcatgat tgaaacaaag ggactggttg cactgatcga
     5161 ggcaagcgat gccatggtca aggcagcccg cgtcaagctg gtcggtttcc agcaaattgg
     5221 cggcggtctg gtgaccgcca tggtgcgcgg tgatgtggct gcctgcaagg ccgccaccga
     5281 tgccggagcg gcggcggcgc aacgcattgg cgagctggtg tcggtgcacg tgattccccg
     5341 tccgcatggc gaccttgaag ccatcttccc gatcaagggt gatggcaagc cgctggactg
     5401 aacccgctga caggagccct ccatgcaact tgcagttgtg gtggggcagg tggtgtcaac
     5461 cgtccgctgt gacggactgg gcaaggaccg cctgcttctg gttgacttta tcaatgccca
     5521 gggccagccg tcgggcctgc gccatgtcgc cgccgacagc gtcggcgcgg gcaacggcga
     5581 atgggtgctg gtggtcagcg gcagttcggc acgcaaggcc aacgacggcg acttgccaat
     5641 tgacctgagt gtcatcggca tcgttgacca ggtggtcgtc gaagaccagg ttacttatcg
     5701 aaaatagtag caggagacgc acgtgaacac tggggatgca gaccttgagc gcattgtcaa
     5761 agcggtgctg caagcaattg atcgcggcgc tggtggcaac accgcaggca gcacagccgt
     5821 tgccgcgcca gccagggctg ccagcgcggc gctacccgca acgccattgc cgcagttgcc
     5881 gatgggcgtg tttgcgtcgc tcgacgatgc ggtggccgag gctgatgttg cataccggca
     5941 aatgcgcagc gtggccatgc gcagccgggt ggtggccgcc attcgtttgg ccggtgaaaa
     6001 gcacgcgcag gaactcgccg agctggccgt ggccgagacc ggcatgggcc gggtggccga
     6061 caagtttgtc aaaaatgtgg cgcaagcgct gcacacgccg ggcgtggaat gtctggtgcc
     6121 gaccgtgctg tcgggcgacc acggcctgac cttgatggaa aacgccgcct ggggcgtggt
     6181 ggcatcggtc accccatcga ccaacccggc cgccaccctc atcaacaacg ccatcagcat
     6241 gattgcggct ggcaatagcg tggtgtttgc gccgcaccct gcggccaagc aggtgtcgca
     6301 gcgcacggtg gcgctgctca accaggcggt ggtggccgct ggtgggccat gcaatgtcat
     6361 cacaacggtg gcgcaaccaa atattgccac cgcccaagcc ctgttttgct accccggcat
     6421 ccacctgctg accgtcaccg gcggcgaagc ggtggtcgaa gaggcccgca aacacaccga
     6481 caaacgcctg attgcagccg gtgcgggcaa cccgccggtg gtggtggacg agaccgccaa
     6541 cctcgaaaag gccgcgcgtg acattgtctg gggagcgtcg tttgacaaca acatcatttg
     6601 cgtcgatgaa aaagaagtca tcgtggtggc atcggtggcc gaccggctca aggcggagat
     6661 gaccaaacac aaggcggttg agttgactgc cgaccaggcc aggc
//
LOCUS       DAOSLL010000200         6673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10412_length_6673_cov_4.649290, whole genome shotgun sequence.
ACCESSION   DAOSLL010000200 DAOSLL010000000
VERSION     DAOSLL010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10412_length_6673_cov_4.649290"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1080
                     /locus_tag="PLB25_10045"
     CDS             136..1080
                     /locus_tag="PLB25_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE41954.1"
                     /translation="MPAISFQSVSKTYPSPRGAQGSSLLALDQVSLDIEEGEFFGLLG
                     PNGAGKTTMITILAGLAKATSGRVSVLGSDVQVDFAQARRKLGVVPQELVFDPFFNVR
                     EALRFQSGYFGVRHNDAWIDELLDSLGLTDKANANMRMLSGGMKRRVLVGLALVHKPP
                     VIVLDEPTAGVDVELRQTLWAFIAKLNKQGSTVLLTTHYLEEAEALCGRIAMLKTGRV
                     VALDSTSALLKAASGNVLRFKIDGDLPVALLPGARVTGRVVQFPAQDALAVERYLAAV
                     REAGLVAHDIEIRRADLEDVFLDVMNRHASPAVQKVAP"
     gene            1077..1832
                     /locus_tag="PLB25_10050"
     CDS             1077..1832
                     /locus_tag="PLB25_10050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE41955.1"
                     /translation="MTGWQMLFYKEVMRFWRVVGQTVGGPILTAMLYLLIFGHALESH
                     VKVYEQVSYTAFLVPGLAMMSLLQNAFANSSSSLIASKVMGNLVFLLLTPLSYLHWFV
                     AYVGASVFRGLVVGLGVFAVSAFFAPISFVAPVWIAVFALMGATLMGALGLIAGLHSE
                     KFEQLAAFQNFIVMPMTFLSGVFYSIHSLPPFWQTVSHFNPFFYMIDGFRYGFFGISD
                     VSPWLSLGVVGSTMLVVSALALTLLRTGYHIRY"
     gene            1915..2157
                     /locus_tag="PLB25_10055"
     CDS             1915..2157
                     /locus_tag="PLB25_10055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HOE41956.1"
                     /translation="MTADQLKSLIAAGLPCAHLEVEGDGRHWYATLVSPEFEGKRAIA
                     RHQRVYATLGDKMKNDEVHALSIKAYTPAEWATLAA"
     gene            2162..3436
                     /gene="murA"
                     /locus_tag="PLB25_10060"
     CDS             2162..3436
                     /gene="murA"
                     /locus_tag="PLB25_10060"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HOE41957.1"
                     /translation="MDKLLIRGGRPLRGEVLISGAKNAALPELCAALLTAEPVTLHNV
                     PRLRDVATMRTLLDHMGVRTETIGERGGMRFHAADPIRPEAPYELVKTMRASVLALGP
                     LLARFGQARVSLPGGCAIGSRPVDQHIKGLTAMGAEIVVEHGYMVAKLPAGRTRLKGA
                     CIATDMVTVTGTENLLMAACLAEGETILENAAQEPEIPDLAEMLIKMGAQIEGHGCSR
                     IRIQGVERLHGCTHQVVADRIETGTFLCAAAVAGGDVLARHGRIDHLEAVVEKLRDAG
                     VIVTAEEGGIRVQSAGAASLHAQSFRTTEYPGFPTDMQAQFMVLNCVAQGTAKVTETI
                     FENRFMHVNELVRLGANVQIDGKVAVVVGVPRLSGARVMATDLRASASLVIAGLVADG
                     ETLVDRIYHLDRGYDQMEAKLCALGADIRRVK"
     gene            3527..4165
                     /gene="hisG"
                     /locus_tag="PLB25_10065"
     CDS             3527..4165
                     /gene="hisG"
                     /locus_tag="PLB25_10065"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HOE41958.1"
                     /translation="MITIALSKGRIFEETLPLLKAAGIEVLEDPEKSRKLILTTNQPD
                     VRVLVVRATDVPTYVQYGGADMGITGKDTLLEHGSQGLYQPLDLRIAKCRISVAVRSD
                     FDYQSAVKKGSRLTVATKYVAISREFFASKGVHVDLIKLYGSMELAPLVGLADAIVDL
                     VSTGNTLKANHLVEVEHIMDISSRLVVNQASLKLKQVPMRHIIDAFESAIGK"
     gene            4542..5231
                     /locus_tag="PLB25_10070"
     CDS             4542..5231
                     /locus_tag="PLB25_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3334 family protein"
                     /protein_id="HOE41959.1"
                     /translation="MVKTGQEFTVVYGTEDLLTSLCNSVTRVLNVATHCQIHYSGMVQ
                     RITKTCLKPDIGCFVLFDGGFSGLVVINFSAPTAMELYESYMLNMGMPKSELASSHTS
                     DEVSDIMGELMNQIVGDFTGKVRRELQTHITQNQPKMLVLNKQVVLSVDTHLDNPEAR
                     RVTFYTGRNNIFYLELAIDRTEFIKLHDFEANEVPNPDELIAQTNAAKQSQPPAAAPV
                     DQDELLKSLGL"
     gene            5417..6598
                     /locus_tag="PLB25_10075"
     CDS             5417..6598
                     /locus_tag="PLB25_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41960.1"
                     /translation="MWFPSWSAGEEVGLDLPGISLQGFGTLGMARSDDDNIEFVRDLS
                     QPHGAGKQWSGKVDSLLGIQANVHFSPSTEGVVQVVSRYHADASFRPELTWAFLRHDF
                     SSDFSLRAGRLGTELYMLGDSRLVGYSNISLRPPPDFYGSLVFSYFDGIDVSATQPLT
                     GGLLKAKLFAGRSPEKSPFYRDILWDLRGSGVAGAYLDYQKGPWQLRVSLGQVRFKRE
                     PPIDETLRVSGDPLSGLPYLALVPEMAMTGQRARFGSLGLVFDQGPFNVQFMLNHIKH
                     DSPAYADSKAGYVLAAYRFGAVIPYAGISRTFTDNAALPSSFIPVVDAVTRSLVSQSY
                     IDQHTYTLGGRWDLQKNLAVKAEVDWIRGTPASVSLFKDTLPGWDGKMTVFSLALDFV
                     F"
BASE COUNT         1271 a         1819 c         2053 g         1530 t
ORIGIN      
        1 ccaggccctg acgctgcgca agacttttgc catccaggga ttgcccgagc ggctaggcga
       61 cttggcgcgc ctgtatggca ttggcgagtt gctgacagcg ccaacggcct gatgccctcg
      121 tagaatagcg gccccatgcc cgccatatcc ttccaatcgg tttcaaaaac ctacccttct
      181 ccacgcggcg cgcagggcag cagcttgctg gcgcttgatc aggtcagtct ggacattgaa
      241 gaaggtgagt tttttggcct gctcgggccc aatggagcag gcaaaaccac catgatcacc
      301 attctggcgg gccttgccaa agccacttcg gggcgggtgt cggtgctcgg cagcgacgtg
      361 caggtcgatt ttgcccaggc caggcgcaag cttggcgtgg tgccgcaaga actggtgttt
      421 gacccgtttt tcaatgtgcg cgaggcgctg cgttttcaat ccggctactt tggtgtcagg
      481 cacaacgatg cctggattga tgaactgctc gacagcctgg gcctgaccga caaggccaat
      541 gccaatatgc gcatgctgtc aggcggcatg aaacgtcggg tgctggtcgg tctggcgttg
      601 gtacacaagc cgccggtgat cgtgcttgac gagcccaccg ccggggtcga tgtggaactg
      661 cggcaaaccc tgtgggcgtt cattgccaag ctcaacaagc agggcagcac ggtactgctg
      721 accacccact acctggaaga agccgaggca ctgtgtggcc gcatcgcgat gctcaagacc
      781 ggccgcgtgg tggcactcga cagcaccagc gctttgctca aggcggcatc gggcaatgtg
      841 ctcaggttca aaatcgacgg tgacttgccg gtggcgctgc tgccaggtgc gcgggtcacc
      901 gggcgggtgg tgcagttccc ggcgcaggat gcgctggcgg ttgagcgcta tctggcggcg
      961 gtgcgtgaag ccggtctggt ggcgcatgac atcgagattc gccgagccga tctggaagac
     1021 gtgtttctgg acgtgatgaa ccggcatgcc agtccggctg tgcaaaaggt ggccccatga
     1081 ccggctggca aatgctgttt tacaaggaag tcatgcgctt ttggcgggtg gtcgggcaaa
     1141 ccgtcggtgg cccgatcctg accgccatgc tgtacctgct gatctttggc catgcgctgg
     1201 aatcgcatgt caaggtctat gaacaagtca gctataccgc gtttctggtg ccaggcctgg
     1261 ccatgatgag cctgttgcaa aacgcttttg ccaacagttc gtcgtcgctg atcgccagca
     1321 aggtgatggg caacctggtg tttttgctgc tgacaccgct gtcctacctg cactggtttg
     1381 tcgcctatgt gggcgcttcg gtttttcgcg gcctggtggt tggcctgggc gtgttcgcgg
     1441 tttcggcttt ttttgccccg atcagctttg tggccccggt ctggattgcc gtgtttgccc
     1501 tgatgggtgc cacattgatg ggcgcgctgg gtctgatcgc aggtctgcat tcggaaaaat
     1561 tcgagcagtt ggctgctttc cagaacttca ttgtgatgcc gatgacgttt ttgagcggcg
     1621 tgttttattc gattcactcg ctgccgccgt tttggcaaac cgtcagccac ttcaacccgt
     1681 ttttctacat gattgacggt tttcgttacg gcttttttgg catcagtgat gtctcgccgt
     1741 ggcttagcct gggcgtggtg ggcagcacca tgctggtggt cagtgcgctt gcgctgacct
     1801 tgctgcgcac ggggtatcac atccgctatt gaaccggcta ttgatactga tagcttcttt
     1861 gttgccccga cattgctgcc ttggcggcga tgacgacttt ttcaccgaac tcttatgact
     1921 gctgaccaat tgaaatccct gatcgccgcc ggactgccgt gcgcgcacct tgaagtcgag
     1981 ggcgacggcc gccactggta tgccaccctg gtgtcacctg aatttgaggg caagcgtgcc
     2041 attgcccgtc accaacgggt ctacgccacg ctgggcgaca agatgaaaaa cgacgaggtg
     2101 catgccctgt cgatcaaggc ctacacaccg gccgaatggg ccaccctggc cgcttgatgc
     2161 catggacaaa ttgttgattc gaggcggccg gccgctgcgc ggcgaggtat tgatttcagg
     2221 ggccaaaaac gccgcgttgc cagagttgtg cgcggcgctg ctgacggctg agccggttac
     2281 gctgcacaac gtgccgcgcc tcagggatgt ggccaccatg cgcaccttgc tggaccacat
     2341 gggcgtgcgc accgaaacca tcggtgagcg cggcggcatg cgttttcatg cggccgaccc
     2401 gattcgcccc gaggcccctt atgaactggt gaaaaccatg cgcgcctcgg tgctggcgct
     2461 ggggccgctg ttggcgcgct ttggccaggc ccgggtgtca ttgcctggcg gctgtgcgat
     2521 tggctcgcgc ccggtcgatc agcacatcaa gggattgacc gccatggggg ccgagatcgt
     2581 ggtcgaacat ggctacatgg tggccaaact tcccgccggc cgcacccggc tcaagggcgc
     2641 gtgcattgcg actgacatgg tgacggtgac cggcaccgaa aacttgttga tggctgcctg
     2701 tctggccgag ggtgaaacga ttctggaaaa cgccgcgcaa gaacccgaga ttcctgacct
     2761 ggccgaaatg ctgatcaaga tgggtgcgca gattgaaggc catggctgta gccgcatccg
     2821 cattcagggt gtcgagcgac tgcatggttg cactcaccag gtggtggcag accgcatcga
     2881 gaccggcact tttttgtgtg cggcggcggt tgctggtggc gatgtgctgg cgcgccatgg
     2941 ccgcattgac cacctggaag cggtggtgga aaaactgcgc gacgcaggtg tcatcgtgac
     3001 cgccgaagag ggtggcatcc gggtgcagtc cgctggtgct gcgagcttgc acgcgcagag
     3061 ctttcgcacc accgaatacc ctgggtttcc gaccgacatg caggctcagt tcatggtgct
     3121 gaactgcgtg gcgcagggca ccgccaaagt gaccgagacg attttcgaga accgcttcat
     3181 gcacgtcaac gagttggtgc gtttgggggc caacgtccag attgacggca aggtggcagt
     3241 cgtggtcggg gtgccacggt tgtccggtgc gagggtgatg gcgacggatt tgcgtgcctc
     3301 ggccagcctg gtgatcgccg gcctggtggc cgatggcgaa accctggtgg accgcattta
     3361 ccacctggac cggggttacg accagatgga agccaaactg tgcgctctcg gggccgacat
     3421 ccggcgggtg aagtgattgg tgcaccgccg ccggtttttc ggatgattga gtttactact
     3481 gaataaatag cgcttagcgc acgttctata agggctacag gccaaaatga tcactattgc
     3541 actgtccaag gggcgaattt ttgaagaaac cctgccgctg ctcaaggcgg ccggcatcga
     3601 ggtgctggaa gacccggaaa aatcccgcaa actcatcctg accaccaatc agcccgatgt
     3661 gcgggtattg gtggtgcgcg ccaccgacgt gccgacctat gtgcaatatg gtggcgctga
     3721 catgggcatc accggcaaag acacgctgct ggagcacggc agtcagggcc tgtaccagcc
     3781 gctggacttg agaatagcca aatgtcgcat cagcgtcgcg gtacgttcgg attttgatta
     3841 ccaaagcgcc gtcaaaaaag gctcgcgcct gacggtggca accaaatacg tggcgatctc
     3901 acgcgagttt tttgccagca aaggggttca cgtcgatctg atcaagctgt acggcagcat
     3961 ggaactggcc ccgctggtcg gtctggcgga cgcgattgtt gacctggtat cgaccggcaa
     4021 cacactcaag gccaaccatc tggtggaggt cgaacacatc atggacatca gctcgcgcct
     4081 ggtggtcaat caggcatcgc tcaagctcaa gcaggtgcca atgcgccaca tcatcgatgc
     4141 gtttgagtca gccatcggca agtagtcggc agggtgacag gcacgctttg acatcggtat
     4201 tgctggtctg cggcgttcgc ccgacaggca aacgcgagcc ggagtccgcg ttgccagcgg
     4261 ttcaatcgga tcggtagcgc tgtctttaaa ttgttctcca tcgactcgtt gctgatgcgg
     4321 ttgacgattt ggcgttttca tcggtaagcc cgggtcttgg cgctttggtt gtcgctcatt
     4381 tggcgttggc accaaaggcg aacgctatcg acttttgaat tctgctgtag cgctagcatc
     4441 gcgagcggcg tttgcaagta ccacaccaaa cgatggaggg cctggtgttg gatggaggcc
     4501 ctggtgctgt acagtcctac ttttaatctg ggaatttaga catggtcaag actgggcaag
     4561 aatttaccgt ggtgtacggc accgaagacc tgttgacgag tctgtgcaac tccgttaccc
     4621 gggtgctcaa tgtcgccacg cattgtcaga ttcactattc aggcatggtc cagcggatta
     4681 ccaaaacctg cctgaaaccg gacatcggct gctttgtgct gtttgacggc ggcttctccg
     4741 gtctggtggt gatcaatttt tcggcaccga cggccatgga gctctacgag agctacatgc
     4801 tcaacatggg catgcccaag agcgaattgg ccagttccca cacttcggac gaggtcagtg
     4861 acatcatggg cgagctgatg aaccagatcg tcggtgactt cactggcaag gtgcgccgcg
     4921 aactgcagac ccacatcacc cagaaccaac caaaaatgct ggtgctcaac aaacaggtgg
     4981 tgctgagcgt ggacacccat ctggacaatc cagaggcccg gcgcgtgacc ttttataccg
     5041 gcagaaacaa tatcttttat ctggaactgg cgattgatcg cactgagttc atcaaactgc
     5101 atgactttga ggccaacgaa gtgcccaacc cggatgagct gattgcccag actaatgcag
     5161 cgaagcagag ccagccgcca gcggctgcgc cagttgacca ggatgaactt ttaaagtcac
     5221 tcggtttgta ggccctgtcg ggtggattgc tgcgggaaaa acaagagcca ttctttttcc
     5281 cttggggtga tttttctgta taaccgggct gatctgggag ttgttcatcg gtatgtttat
     5341 cgctcaaata attagaacca ttgagttttc gcagcgcttg acgcgacagt tggtcaagct
     5401 gttggctgtt ctggtcatgt ggtttccatc ctggtcggca ggggaagagg tcggtttgga
     5461 tttgcctggt atcagcctgc agggcttcgg tacgctgggc atggcccgct ctgatgacga
     5521 caacatcgaa ttcgtgcgcg atttatcgca accgcatggt gcgggaaaac agtggagcgg
     5581 caaagttgac agcttgcttg gcattcaggc caatgtccat ttcagcccaa gcaccgaagg
     5641 cgtggtgcag gtggtcagcc gctaccacgc agatgccagc ttccgtccgg aattgacctg
     5701 ggcgtttttg cgacacgatt tttcgtccga tttctccctg cgtgccggtc gcctgggcac
     5761 tgagttgtac atgctggggg actctcgtct ggtcggttat tccaacatca gcttgcggcc
     5821 accgccggat ttttatggtt cgctggtttt ctcatacttc gatggcattg atgtcagcgc
     5881 gacccaaccg ctgacaggcg gcttgctcaa agcgaagttg ttcgccggca ggtcacctga
     5941 gaaatcaccc ttttatcggg atattttgtg ggatttgcga ggttcaggtg tggctggtgc
     6001 ctatctggat tatcagaaag gtccttggca acttcgggtc agccttgggc aggtccgctt
     6061 caagcgggag cctccgatcg atgaaacgtt gcgtgtcagt ggtgatccac tttcgggcct
     6121 accctatctt gcgttagtgc cagaaatggc gatgaccggt caaagggcac gctttggttc
     6181 gcttgggctg gtgtttgacc aggggccgtt taacgtccag ttcatgttga accacatcaa
     6241 acatgacagc ccggcctatg ccgactcgaa agccggttat gtgctggctg cctatcgttt
     6301 tggtgcggta ataccctacg cgggcatttc tcgcactttc accgacaacg cagcgctgcc
     6361 ctccagtttt atcccggtag ttgatgctgt gacgcgatca ctggtgtcgc aaagctatat
     6421 cgatcagcac acctacacct tgggcgggcg ttgggatctg caaaaaaatc ttgccgtcaa
     6481 ggccgaggtg gactggatac gcggcacacc ggcatcggtc tctttgttca aagacaccct
     6541 gcccggatgg gatggcaaga tgaccgtttt cagcctggcg ctggattttg tgttctagcc
     6601 atgcaagtgc tgttgttatc ccttttggtg gcgctgctgg gcacaggcgc tgcggtcaat
     6661 gccgaaccgg tgg
//
LOCUS       DAOSLL010000201         6673 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10414_length_6673_cov_3.164551, whole genome shotgun sequence.
ACCESSION   DAOSLL010000201 DAOSLL010000000
VERSION     DAOSLL010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6673)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6673)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6673
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10414_length_6673_cov_3.164551"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="PLB25_10080"
     CDS             complement(<1..1515)
                     /locus_tag="PLB25_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020201299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HOE41961.1"
                     /translation="MSPLPGLRQDVRPDAFPGEAELAALRGWYAGLSSRESVARYLAA
                     HKVSGQSSRGMLGQIRRQLISIARQRHRADLVVLLDHPESERIQHARTVGHAIDALRT
                     APEPTPRITDDVSLWLSARSTNALYAQGIETLAQLTVRVPRRRRWWTVVSGLGTKGAR
                     QIEAFFAAHPQLTERARELVVSERTQTIVPWERLEVPDELNGSRGSFRAPPSSCTLSA
                     DNDYEAVQAWLALHESSATKRAYRKEAERLILWAIVERGRALSSLTTEDATAYRGFLR
                     NPTPRSRWVGPPQTRFSSEWRPFAAGLSPRSTAYALTVIGAMFRWLIEQRYVLANPFA
                     GIKVRGGGRSTPMDEGRVFSEGEWAIIRSMANGLEWSYGWQVPAAQRLRFMLDFAYST
                     GLRSSEIVGASLGQIETDVQGDHWLKLVGKGSKAGKVALPPLARVALDHYLMQRGVPT
                     TPALWKPSTPLIADLAAGGGQSRITSTRLWDVTKRFFVATAEVIEAESPKTAEKL"
     gene            1719..4814
                     /locus_tag="PLB25_10085"
     CDS             1719..4814
                     /locus_tag="PLB25_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HOE41962.1"
                     /translation="MSPIDSHRLSILSKSEVHALYGMPVFSEPDREHYFDMSPAEMAS
                     AQARTQGLGVFQALELGYFKAKRQFFNFQPIDVQADLHHIAHRHFWDIDTKDLRLPVA
                     KTRARIQQAILELCAYRAFDASASAELTNRLLRMAAISTHPAYLLREALQHLESHRVV
                     APSYSTLQDLIGAVLERESQRVTRIMDEHMPVDVKKTLDDLLQGEDGDMLTFNAIKRE
                     PKSFSVKELSHEVQRRQFFAPAHEFALSFVPLAGVSSESIKYYASLVQFYTRYKLRRM
                     ASTSARLYLLCFCFHRFRQINDNLIEAFIHLVGQFDKQARAVANEAMQTAISQANTNL
                     LAAGEVLSLFVDESIAEDCPFSAVKARAFELLDPTAFPAVANYLRNIAFDRAAFEWAY
                     FAKLSMTIKRNLRHLFKELDFAGRVDEAPLMGAITVMQELLRQSKSLRQADAAKFPEA
                     VIPQALKKHIFVETIVDGTKHKALDVDRYEFLVYRLLRNALESGDLYVNHSNEFRQFE
                     DDLISDARWAHKDAVLAEIAQPFLLAPIEETLAALRTKLEARFVEVNERVATCVNANI
                     KVVGQGEKKRWSLIYPTAPETGNSPFYNQIPGIGIANLLRFVNGETHFLDAFEHVLNR
                     GVKHAPDLREILACVVAFGTNMGLGKMAEVSGLSHAALIATARSYLRPETVHAANDAI
                     SNATAMLPAFKLFNIREALHSSSDGQRFETQINTFNARHAPKYFGLDKGVSACTVVAN
                     HVPINARIIGTHEHESHFVFDLLYNNTSDIKPTRHSTDTHGTNQVNYLCLFAHGYSFA
                     PRYKTVQKKTASLVGFNLPSHYPADMLIRPTSKANEDMIISEWPNVQRILASLAQKDT
                     TQATIVRKLSSYARQNNTKKALWELDNILRTIYILDFIDDVELRQSVQKALNRGEAYH
                     RFRRAVAYVNGGKFKVQTETEQQVWNDCARLITNAVIYYNTALLSKVYEQKVASGDLD
                     AMAFIQGMSPVAWQHVNMFGSFEFSDEDPDIDLEALAAQYADAVFWSNATQPGQGDLF
                     D"
     gene            4925..5209
                     /locus_tag="PLB25_10090"
     CDS             4925..5209
                     /locus_tag="PLB25_10090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6516 family protein"
                     /protein_id="HOE41963.1"
                     /translation="MPAQLITRFKDVTPDGAILEVVIWKVPKPVPPTDHGYKYSAVYV
                     MDGVRIVGFDNERGKGDHCHLDGGEVPYTFTGVDQLIEDFIAAVAARRAP"
     gene            5206..5559
                     /locus_tag="PLB25_10095"
     CDS             5206..5559
                     /locus_tag="PLB25_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41964.1"
                     /translation="MTERYLTITLQPDWKGALRAMAKTAQADKYQGEVLNFESPGHFF
                     GQLSEKRWEIVRAAQGKGELSVRELARAVGRDVKRVHEDVVILAELGLLDRTESGGVL
                     CPYSSMHIDMYLKAA"
     gene            complement(5592..>6673)
                     /locus_tag="PLB25_10100"
     CDS             complement(5592..>6673)
                     /locus_tag="PLB25_10100"
                     /inference="COORDINATES: protein motif:HMM:NF022580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraC family protein"
                     /protein_id="HOE41965.1"
                     /translation="TFWEETVLGNVSHVGARPAPVAAADDVAQIYGLHSILKYDQFDT
                     SNDLFYNETSVGFCFEVLPQTGADAEMAERLSTLFTPIPPGYGVQWCFFASPMLDEQY
                     GAYMDLRHIAKDKGLTDPLFLELANKRITSMRRNPGRPLFANDNFMAKNLRLVFSITK
                     TGSPKDRTLVAEILEIRETVKAALSGANLPSHTLGAQGLIEFLWPILNPGAMFSPEML
                     PRLEYDPGKILKNQMTAFGQHVRVTSKEILFGLPPENAGDEDKRIAVRALGVLRYPQK
                     KELWEMANIIGSFFDDSLQYPCPFLICGGVFTLDANISDAKAQFRSGRAKMNAKSKMA
                     EYQPELESKALDWLNAPIPRKSDTL"
BASE COUNT         1486 a         1947 c         1847 g         1393 t
ORIGIN      
        1 tagcttttca gccgttttcg gggactctgc ctcaatgact tcggcggttg ccacgaagaa
       61 acgtttggtg acatcccaca gcctggtcga tgtaattcga ctttgaccac ctcctgccgc
      121 cagatcagca atgagcggag tagatggttt ccacaatgcc ggcgtggtgg gcacgccgcg
      181 ctgcatcaag taatggtcca gcgccacgcg cgccagcggc ggtagcgcca ccttgcccgc
      241 tttgctacct ttacccacca gctttagcca gtggtcgccc tggacatccg tttcaatctg
      301 ccccaggctc gcacccacaa tctcactgga acgtagcccg gtggaataag cgaagtccag
      361 cataaagcgc agcctttggg ccgccggcac ttgccacccg taagaccact ccaacccgtt
      421 cgccatggaa cgaatgatcg cccactctcc ctcggagaat acacgccctt cgtccatggg
      481 cgtgctgcga ccgccaccgc gcactttgat gccagcaaac gggttggcca gcacatagcg
      541 ctgctcaatc agccagcgaa acatcgcccc aatgacagtc aaggcgtatg ctgtggagcg
      601 tggcgaaagc ccggctgcga acggccgcca ctcagaagag aatcttgtct gaggaggtcc
      661 aacccatcgg ctgcgtgggg tggggttgcg taagaaccca cgatatgccg tcgcgtcttc
      721 ggttgtgagt gacgatagtg cgcgcccgcg ttcaacgatg gcccacagaa tcaggcgttc
      781 agcctccttg cgataggccc gcttggtcgc gctggactcg tgcaatgcca gccaggcctg
      841 cacggcctcg tagtcattgt cggcactgag ggtgcaactg gacggcggcg cacgaaagct
      901 gccacgcgaa ccgttgagtt cgtctggcac ctccaaccgc tcccacggga caattgtttg
      961 cgttctttcg gaaaccacca actcacgcgc tcgctcggtt agctgcggat gggctgcaaa
     1021 gaaggcttcg atttggcgtg ccccttttgt ccccagtcca gaaaccacgg tccaccatcg
     1081 ccgccgccgg ggaacacgca cagtcagttg tgccagcgtc tcaatgcctt gcgcatacaa
     1141 agcattggtc gatcgcgcgg acaaccacag gctcacatca tcggtgatcc taggcgtcgg
     1201 ttcaggggca gtgcgcagag catcgatggc atggccaaca gtccgcgcat gctggatacg
     1261 ttcactctcg ggatgatcaa gtaggaccac cagatctgct cggtgccgtt gacgtgcaat
     1321 gctgatcaat tggcggcgga tttgcccaag cattccccga gacgactgac ccgatacctt
     1381 gtgcgcagcc aggtaacgag ccactgattc acgtgacgac agaccggcgt accaaccacg
     1441 cagggcggcg agttcggctt caccggggaa tgcatctggg cggacatcct gcctcaaccc
     1501 tggcaagggg gacatcggcg gatttttcat ggatgccagt tcgtgtgttg caaatactca
     1561 cagtgataat aatacttatc gtatgagtca ctagaatctg ccacttattg tcgctcagag
     1621 caagcgcaaa tttcatttat ttgggtgctg ctgagccacg cattgggtcc cattgaccaa
     1681 tgcgtgaccc tgcgcacccg catctccaac gcccgcgcat gtccccaatt gattcacacc
     1741 gcctcagcat cctctccaaa tccgaagtcc acgcgctcta tggaatgccg gtcttcagcg
     1801 aaccggaccg agagcactac tttgatatga gcccagcgga aatggcatcc gcgcaagcca
     1861 gaacacaggg cttgggagtc ttccaggcgc tagaactggg gtacttcaaa gccaagcggc
     1921 aatttttcaa tttccaaccc atcgacgtgc aagctgactt gcaccatatc gcacataggc
     1981 acttctggga cattgacacg aaagacctgc gcctgcccgt tgctaaaaca cgcgccagaa
     2041 ttcagcaagc catccttgaa ctctgcgcct accgggcgtt tgacgccagc gcctcggcag
     2101 aattgaccaa caggctgctg cgtatggcgg caatatcaac gcacccggcc tacctgctgc
     2161 gcgaagcact gcagcatctc gaaagccacc gcgtcgtagc ccccagctac agcacgttgc
     2221 aagacctcat tggcgcagtg ctggagcgcg aaagccagcg cgtcactcgg atcatggatg
     2281 agcacatgcc agtggatgtc aaaaaaacct tggacgattt gttgcaggga gaagatggcg
     2341 acatgctcac tttcaacgcc atcaagcgag aacccaagag cttcagcgtc aaggagctca
     2401 gccatgaggt gcaacgacgc cagttcttcg ccccggcaca cgaatttgcg ctcagctttg
     2461 tgccgcttgc cggtgtttca agtgagagta tcaagtacta cgcctcgctg gtccagttct
     2521 acacacggta caaattgcgg cgtatggcgt cgaccagcgc ccggctgtac ttgctgtgtt
     2581 tttgcttcca ccggttccgc cagatcaacg acaacctgat cgaggcattc attcatctgg
     2641 tcggtcaatt tgacaagcag gccagggccg ttgcaaatga ggccatgcag acggcgattt
     2701 cgcaggccaa taccaacctg ttggcagccg gtgaggtcct gagcctcttt gtggatgagt
     2761 ccattgcgga ggattgccca ttttccgcag tcaaggcgcg cgcctttgaa ttgcttgatc
     2821 ccacggcatt tccggccgtt gccaattacc tgcgcaacat tgcctttgac agagcagcct
     2881 tcgaatgggc ttactttgcc aagctgtcaa tgaccatcaa gcgcaacctg cgccatctgt
     2941 ttaaggaact cgattttgca ggccgcgtcg acgaggcacc cttgatgggc gccatcaccg
     3001 tcatgcaaga acttttgcga caaagcaaat cgcttcgcca agcagatgcc gcgaagttcc
     3061 ccgaggccgt cataccccag gctctcaaaa agcacatttt tgttgaaacc attgtggacg
     3121 ggacgaagca caaggctctg gatgtcgacc gctacgagtt tttggtatac cggctactgc
     3181 gcaacgcact ggagtccggg gacttgtacg tcaaccacag caatgaattc cgtcagtttg
     3241 aagatgacct gatcagcgac gcccgttggg cgcacaaaga cgctgtgctg gcagaaatcg
     3301 ctcagccctt tctgctggcg cccattgaag aaaccttggc cgcattgcgc accaagctcg
     3361 aagcgcggtt cgtcgaggtc aatgagcgcg tggccacctg cgtcaatgcg aacatcaagg
     3421 ttgtcggtca aggcgagaag aagcggtggt ccctgatata cccgacggct ccggagaccg
     3481 gcaatagccc gttttacaac cagatccctg gtattggcat tgcaaatctg ttgcgcttcg
     3541 tgaacgggga gacacatttc ctggatgcct ttgagcatgt gctgaatcgc ggtgtcaagc
     3601 acgcgccgga cctgcgcgaa atcctggcct gcgtcgtggc gttcggcacc aacatggggt
     3661 tgggcaagat ggcggaggtt tctggcttga gtcatgcggc cctgattgca acggcgcgca
     3721 gctacttgcg gccagaaacg gtgcacgctg ccaatgacgc gatcagcaat gccaccgcca
     3781 tgctgccggc cttcaagctc ttcaacatcc gcgaagcgct gcattccagc agcgatggcc
     3841 agcgtttcga gacccagatc aacacattca atgcccgaca tgcgccaaag tatttcggcc
     3901 tggacaaggg tgttagcgcc tgcacggtgg ttgccaacca cgtgcccatc aacgcccgga
     3961 ttatcggcac ccatgagcac gagagccatt ttgtgttcga tttgctgtac aacaacacgt
     4021 cggacatcaa acccacgcgc cactcgaccg atacgcatgg caccaaccag gtcaactatc
     4081 tgtgcctctt tgcccatggc tacagttttg ctccgcgcta caagacggtg cagaagaaga
     4141 ccgcatcctt ggtgggcttc aacctgccaa gccattaccc ggccgacatg ctgattcgac
     4201 cgaccagcaa ggccaacgag gatatgatca tcagcgaatg gcccaatgtc caacgcatcc
     4261 tggcgtccct tgcgcaaaag gacacgacgc aggccaccat cgtgcgcaaa ctcagcagct
     4321 atgcccggca aaacaacacc aaaaaggcgc tgtgggagtt ggacaatatt cttcgcacca
     4381 tctacattct ggacttcatt gacgatgtgg agttgcgcca gagtgtgcaa aaggcgctca
     4441 accggggtga ggcctaccac cggttccgcc gcgcggtggc ctacgtcaac ggtggcaaat
     4501 tcaaggtcca gacggaaacc gagcagcagg tctggaatga ttgtgcgcgg ctgattacca
     4561 acgcggtcat ctactacaac acggcgctgc tgtccaaggt gtacgaacag aaggtggcct
     4621 ccggggattt ggacgccatg gcattcatcc agggcatgtc gccggtggcg tggcaacacg
     4681 tgaacatgtt cgggtcgttc gagttcagcg acgaagatcc ggacattgac ctggaggcgc
     4741 tggctgcaca atatgccgat gcggtgttct ggagcaacgc aacgcaacct gggcaagggg
     4801 acctttttga ttgatggctt ccaatcgtcc acgagataca ccgatcattt cttcctcact
     4861 ctcggctgac ctttaagtgt tggcaatatt gacaacaaat gcgaatgttg tcacaattgc
     4921 caacgtgcct gcccaactca ttacccgctt caaagacgtc acgcccgacg gcgcaatact
     4981 tgaagtggtg atttggaaag tacccaaacc tgtaccgcct acggaccacg gttacaagta
     5041 cagcgccgtg tatgtgatgg acggcgtgcg catcgtcggt tttgacaacg agcgcggcaa
     5101 aggtgaccac tgtcaccttg atggcggtga agtgccctat acattcaccg gcgtggatca
     5161 actgattgaa gactttattg cagccgtcgc tgccaggaga gcaccatgac agagcgctat
     5221 ctgaccatca cactgcagcc tgactggaag ggtgctttgc gcgccatggc caaaacggct
     5281 caggcggaca aataccaggg agaagtgctc aacttcgaaa gtccaggaca tttctttgga
     5341 cagctttccg aaaaacgttg ggagatcgtt cgcgcggccc agggcaaagg ggaactgtca
     5401 gtgcgcgaac tcgcccgtgc agtgggacgc gatgtcaagc gcgtgcatga agacgtggtt
     5461 atcttggcag agctgggctt gctggaccgt acggagagcg ggggcgtgct ttgcccctac
     5521 tcatctatgc atatcgacat gtacttgaaa gcggcttgat aacgcgaggc gacgtgcgat
     5581 tggcaccaac tttacaaagt gtccgatttt cgggggatgg gcgcatttag ccagtccagc
     5641 gccttgcttt ccaactcggg ctggtactct gccattttgg acttggcatt catcttggcc
     5701 cgtccgctcc tgaactgcgc cttggcatcc gagatgttgg catcgagcgt aaacaccccg
     5761 ccgcagatca ggaatggaca gggatactgc agggaatcgt caaaaaatga gccaatgatg
     5821 ttggccatct cccagagttc ttttttctgg ggataacgca gcacacccag ggctcgcacc
     5881 gcgatgcgct tgtcttcgtc accagcattt tctgggggca gcccaaacag gatttccttg
     5941 gaggtgaccc gcacatgctg gccaaacgct gtcatctggt ttttgaggat cttgccgggg
     6001 tcgtattcca gtctgggcag catctccggg ctgaacatgg cgcctggatt gagaattggc
     6061 cacaaaaact cgatcaaacc ctgcgcgccc agcgtgtggg atggcaagtt ggcaccagag
     6121 agggccgcct tgaccgtttc gcggatttcc agaatctcgg cgaccagggt gcggtccttg
     6181 ggcgatccgg tcttggtgat ggagaacacc aggcgcagat tttttgccat gaagttgtcg
     6241 ttggcaaaca gtgggcgtcc cgggtttctg cgcatggagg tgatgcgttt gttggcgagc
     6301 tccaaaaata gcgggtcagt aagccccttg tccttggcaa tgtggcgcag gtccatatat
     6361 gccccgtact gctcgtccag catggggctg gcaaaaaagc accactgaac gccatagcct
     6421 ggcggaatgg gtgtgaacag tgtcgacagg cgctctgcca tctcggcatc ggccccggtt
     6481 tggggcaaga cttcaaaaca aaacccgacc gaggtttcgt tgtaaaacaa atcattgctg
     6541 gtgtcgaact ggtcgtattt gagaatgcta tgcaacccgt aaatctgcgc cacatcgtca
     6601 gcagcggcta ccggtgcagg acgcgcacca acgtgagaga cattgcccag gaccgtttct
     6661 tcccaaaacg tcg
//
LOCUS       DAOSLL010000202         6665 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10432_length_6665_cov_4.869440, whole genome shotgun sequence.
ACCESSION   DAOSLL010000202 DAOSLL010000000
VERSION     DAOSLL010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6665
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10432_length_6665_cov_4.869440"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..151
                     /locus_tag="PLB25_10105"
     CDS             <1..151
                     /locus_tag="PLB25_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HOE41966.1"
                     /translation="DFTVKRLYQRAGRVKLKAANPTFADIVPKEGQTIEVWGVVTSSI
                     KQFPV"
     gene            180..1478
                     /locus_tag="PLB25_10110"
     CDS             180..1478
                     /locus_tag="PLB25_10110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011458712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HOE41967.1"
                     /translation="MYALVDGNNFFVSCERVFRPSLNGLPVVVLSNNDGCAIARSNEA
                     KALGIAMGAPWFQIKHLVEDAGLVALSANFALYGDLSDRMMSLAAGLGPQQEIYSIDE
                     SFIDLTGVRGDLVARSHKIRSRILQWVGIPCGIGIGATKTLAKLANHVAKTAERKPGS
                     YSAQLAQVCDLSALRQSELEAVLVATPVNEVWGVGRQISKQLTAAGVNTVLDLVRLDP
                     VMVKRRWSVVLERTVRELQGMPCIELEHSPRAKQEIACTRSLGHPVTELTALAEAVTE
                     FASRAAVKLRKQGSLAALVMVFIRTSPFRKDPQCSRSTTVPMRRPTSDTAAILEAALA
                     GLRLIYQPAFNYAKAGVMLLDLQSDQVQQGELDWNDGAGKHRGHLMSSLDGLNQRFGR
                     GTVKLASAGLQGDRSVWSMKQERRTPAYTTCLADMPTAKA"
     gene            1496..2227
                     /locus_tag="PLB25_10115"
     CDS             1496..2227
                     /locus_tag="PLB25_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41968.1"
                     /translation="MQSLPELKRHHAKRLREVYRSAGWPCLDVIEIELLAAGLLERHS
                     LDDHAHVRVTDAGIQYLASAAHSNRQAMSAHEALVDQVTQVLLRDGRLVWKNLNLRAW
                     VAPNDDTPGRWKMCLPDVFSIRNSSVQAYLEPIVHEIKVSRADLLGDLKRSDKRAAYL
                     DLGGQCWYVLGCDRSGHPIAKPNEIPLECGVMVYQGNQLEVMRMAPKRPAPELPFGVW
                     MALAKATPMQMSGLDNCQGTLPYQS"
     gene            2698..4542
                     /locus_tag="PLB25_10120"
     CDS             2698..4542
                     /locus_tag="PLB25_10120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE41969.1"
                     /translation="MRKLVRILFLLWLTSFSTLTHTAEPLVLDERLEKLDLASVLSAF
                     EDSAGTLTIEEARRQFAIGRFSPVDPKKGFNEGYTRSAWWLAFQVRLPVGSHLANFPW
                     LLELAFPSLDKVEVFRPGSDTPIRTGDTYPFSERPMRHVNFVFPMTLATPDVEAIFLR
                     VQSEGSLIAPLTAWSPETFAASSRLAYAGQAAYFGALLAMVIYNALLWFSVRERAYID
                     YVLYVTFIGLGIGAANGMAAEFFWPDSGWLVNYSYWAFGFAGIFVTRFARSFLNTVVT
                     SIWLDRALRAAGILVAVPIVIGLFVSYYAGGRLVSGALFIALLVSAGSGIYSVIHKIP
                     GGRLYLLSWFLFLLFATMYPLRNYGLVPTNFFTVHGIQIGSLAEMMLLSFALAARINH
                     TRAEKEKAQHEAIDAQSALVATLKETEKILEQRVAERTEQLEQANRRLSSLSITDGLT
                     GIANRRRFDEVLAEEWKRMERLGQPLALAMIDIDWFKKYNDHYGHQAGDACLVAVAEM
                     IAASIGRTGDLVARYGGEEFSFIAPAVDGASALTMAQKVCNAISATGMPHEKSLFSHV
                     TVSVGVASIVPSIETEPESLLKLADEALYLAKDSGRNRAMMKLHVNAT"
     assembly_gap    5048..5057
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5075..5968
                     /locus_tag="PLB25_10125"
     CDS             5075..5968
                     /locus_tag="PLB25_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOE41970.1"
                     /translation="MSRSASAHRSETLKALYPLLFVALWSTGFIGAKFGLPYAEPLTF
                     LTIRYLAVLVLMGAITWRMRAPWPRTGLEWIHIGISGLLIHALYLGGVFTAISHGLPA
                     GVTSLVVGLQPVLTALGAGLLLGERVRPTQWAGLALGFVGVGLVVAHKVVASASGGAL
                     LVMLLPAAIALLGITVGTLYQKRFCQAFDLRTGSVIQFMPCLAVTALVASQTETMQVQ
                     WADSFMFALGWLVLVLSLGAVSLLNVLIRSGSAVNVASLFYLTPPTTALIAWALFDET
                     LTGLALIGMGLTVFGVWMARK"
     gene            6458..>6665
                     /locus_tag="PLB25_10130"
     CDS             6458..>6665
                     /locus_tag="PLB25_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41971.1"
                     /translation="MGTPSLIGTPLTNIGKLTIEYKCYCVFTINGRKCVMVSSLTTPQ
                     DLNLSVAETVDLLTKELGGESSRWA"
BASE COUNT         1334 a         1844 c         1926 g         1551 t
ORIGIN      
        1 tgacttcacc gtcaagcggc tgtatcagcg tgccgggcgc gtcaagctca aagccgccaa
       61 cccgaccttt gccgacatcg tgcccaagga aggccagacc atcgaggtct ggggtgtggt
      121 gaccagcagc atcaagcaat ttcccgtctg acagcccgga cccgtcagcg agcacgttca
      181 tgtacgccct ggtggatggc aacaacttct ttgtgagctg tgagcgggtg tttcgcccca
      241 gcttgaacgg actgccggtg gtggtgttgt ccaacaacga cggttgcgcc atcgcccgct
      301 ccaacgaagc caaggcgctg ggcatcgcca tgggtgcccc ctggttccag atcaagcacc
      361 tggtcgaaga tgcgggactg gtggccctgt ctgccaactt cgccctgtac ggagacttga
      421 gcgaccgcat gatgagcttg gcggctggtc ttggccccca gcaagagatc tattccattg
      481 acgagagttt tattgacctg accggtgttc gcggtgatct ggtggcacgc agccacaaga
      541 tacgttcgcg catcctgcaa tgggtaggca tcccctgtgg cattggcatc ggtgccacca
      601 agactctggc caaactggcc aaccatgttg ccaagactgc cgagcgcaag cccggtagct
      661 atagcgccca actggcgcaa gtgtgtgact tgtcggcact gcgccaatcg gaactggagg
      721 cggtgttggt tgccaccccg gtcaacgaag tctggggggt tggcaggcaa atctccaagc
      781 agttgacagc ggccggagtg aacaccgtgc tagacctggt gcgcctggat ccggtcatgg
      841 tcaaacgacg ctggtcggtg gtgctggaac gtacggtgcg cgagttgcaa ggcatgcctt
      901 gtattgagct tgaacattca cctcgggcca aacaggaaat cgcctgcacc cgctcacttg
      961 ggcacccggt gactgagctg acggcactgg ctgaagccgt gactgaattc gccagccggg
     1021 cagcggtcaa gctcagaaaa caaggtagct tggctgcgct ggtcatggtg ttcatccgca
     1081 ccagtccatt tcgcaaggat ccgcagtgca gccgctcaac gacagtgcca atgcgtcgac
     1141 caacttcgga taccgctgcg attcttgaag cagccttggc aggtttgcgg ctcatctacc
     1201 aaccggcctt caactacgcc aaggccggtg tcatgttgct ggatctgcaa tcggatcaag
     1261 tccagcaggg ggaactcgac tggaatgatg gtgctggcaa acaccgtggt cacctgatgt
     1321 cttcgctgga tggcttgaac cagcgatttg gacgtggcac agtgaagctg gccagtgcgg
     1381 gcctgcaagg tgatcggtct gtctggtcga tgaagcagga gcgccggacc ccggcttaca
     1441 ccacttgcct ggcagacatg ccaacagcaa aagcctgagt tgaggagcac acaacatgca
     1501 gtccttgcct gaactcaaac gtcatcatgc caaacggctg cgcgaggtgt atcgctctgc
     1561 ggggtggcct tgcctggatg tgatcgagat tgagcttctg gcagccggac tgctggagcg
     1621 ccacagcctt gatgaccatg cccatgtgcg cgtgacagat gccggtatcc aatacctggc
     1681 cagtgccgcc catagcaatc ggcaagcgat gtcagcccat gaagccctgg tggatcaggt
     1741 gacccaggtg ttgctgcgcg acgggcgatt ggtgtggaag aacctgaacc tgcgtgcctg
     1801 ggtcgcacca aatgacgaca cacccgggcg ctggaagatg tgcctgcccg atgtgttctc
     1861 cattcgtaac agcagtgtgc aggcgtacct ggagcccatc gtgcatgaga tcaaggtcag
     1921 tcgtgccgat ttgctgggag atctcaaacg gtccgacaaa cgcgctgctt atctggacct
     1981 gggcgggcaa tgctggtatg tgctgggttg tgatcgtagt ggccatccga ttgccaagcc
     2041 caacgagatt ccactggagt gtggggtgat ggtgtaccag ggcaaccagt tggaggtgat
     2101 gcggatggca cccaagcggc ctgccccgga gttgcccttc ggtgtctgga tggcgctggc
     2161 caaagccaca ccgatgcaga tgtctggctt ggacaattgc caggggacgc tcccatatca
     2221 gtcttgatag cgatttcgca gtggcggcga ctggtgtgcc taaacctctg agaatgactt
     2281 gtctcaattt tgttgactgc ggatggtcga tacatttctc agggatggca actggtcttt
     2341 gctgaaaaca tggaccagcg gtgcctgcgg cagaatttga gggttgtccg gcggcaattt
     2401 tgctggcagc caatggcagc gtcagacccg ttgccgacag tcagatttct ggcgcgaggg
     2461 gtgggtttcg acccattgcg gtcgtagcga ctgcctaaat cgacgcccca tagccgacat
     2521 ccacgcatct gcagtatctc gctaccaatg tctgttgtcg agatggccca acttcaaagt
     2581 ggtcgtcacg agttggccag attctgcaca taacaccgcc tgctctggcg tgccagaatg
     2641 gcgaaaaacg attgatatgg ctgggcagta accatttccc ttacttcgat tgaatccatg
     2701 cgtaaacttg ttcgcatcct tttcttgctg tggttaacca gcttttcgac gttgacgcac
     2761 acggccgaac cgcttgtcct cgatgaacga ctggagaagt tggaccttgc ctctgtcctt
     2821 tctgcctttg aagattctgc cgggacgctc acgatcgagg aggcgcggcg tcagtttgct
     2881 atcggtcgct tcagcccggt cgaccccaaa aaaggcttca atgaaggcta tacccggtcc
     2941 gcatggtggc tcgcctttca ggtgcgcctg ccagttggga gtcacctggc taattttcct
     3001 tggttgttgg agttggcttt tccgtcgttg gacaaggtcg aggtctttcg gccgggctcc
     3061 gatacgccca ttcgaaccgg agatacctac ccatttagcg aacgtccgat gcgccatgtg
     3121 aactttgtct ttcccatgac actcgcaacg ccagacgtag aagccatttt cttgcgtgtt
     3181 caaagcgagg gctcgttgat agctccgctt accgcctggt cgccagagac gttcgctgcc
     3241 tccagccgtc tggcctatgc gggtcaagca gcctattttg gggcactcct ggcgatggtt
     3301 atctacaacg ccctgctgtg gttcagcgtg cgtgaacgtg cctatattga ctacgtgctt
     3361 tacgtgacat tcatcggctt gggtatcgga gcagcgaatg gcatggccgc cgagttcttc
     3421 tggcccgata gcgggtggct ggtgaattac tcctattggg cgtttggctt tgcgggaatt
     3481 tttgttaccc ggtttgcccg tagttttctc aacactgtag tcacgtcgat ctggctggac
     3541 cgcgccttgc gtgcagccgg cattctggtg gcggtgccca tcgtcattgg tttgttcgtc
     3601 tcgtattacg ccggtggccg gctggtctca ggtgctttgt tcatcgccct tctggtgtca
     3661 gcagggtcag gcatttacag tgtgattcac aaaattccgg gcggtcgcct gtatctgctg
     3721 tcctggtttt tgtttctgtt gttcgcgacg atgtatccgc ttcgcaacta tggcctggtt
     3781 cccaccaact ttttcaccgt ccatggcatt cagattggct cgctggccga gatgatgctg
     3841 ctttcatttg ccttggccgc tcgcattaac catactcgcg ctgagaagga gaaagcccag
     3901 cacgaggcaa ttgacgcgca gtctgctctg gtcgccacgc tcaaggaaac cgagaaaatc
     3961 cttgagcagc gggtcgccga acgtaccgag caacttgagc aagccaaccg ccgtctgagt
     4021 tcgctgagca ttaccgatgg cttgaccggc attgccaacc gccgacgttt tgacgaggtc
     4081 ttggcggagg aatggaaacg catggagcga cttgggcagc ctcttgcgct ggcgatgatc
     4141 gacattgact ggttcaaaaa atacaacgat cactatgggc atcaggctgg cgatgcctgt
     4201 ctcgtcgcag tggccgagat gattgcagcg agcattggac gaactggtga tctggtcgca
     4261 cggtacggcg gcgaagaatt ctcgttcata gcccccgcag tggatggtgc aagtgcgctg
     4321 acaatggctc aaaaagtgtg caatgccatc tccgcaacag gcatgcctca tgaaaaatca
     4381 ctgttctcac acgtcaccgt gagtgtgggt gtggcttcca tcgtcccatc tatcgaaact
     4441 gaacctgaaa gtttgctcaa gctggcagac gaagcactct acttggcaaa ggactcaggt
     4501 cgcaatcgag ccatgatgaa acttcacgtc aacgcgactt gacgcaaata gcatttctgc
     4561 ttcacgaatc aacaacgcga ggaccatgtc cgcaatctat ttttgtatcg gtacagacca
     4621 aaaacgtatc aaactagacg cctattggtc aacccatact ggggtgcaga caattttctg
     4681 gcgattagcc agagtctctc cgctggagcg gaatggttga ccgccatgac ccaccggatc
     4741 atgcgccctc cgacatttgt ccttgacaac agtgggcgac cggttagcgc gccgccgatt
     4801 gaatgattca ggcactatcc ggttgttgac gacggtcctg tttctggctt cttgttaagt
     4861 caaatttaag gtgctccaga acggccattg atgtccgacg gcacctggcc caaaccgggt
     4921 gctcccgatt ttgattcgtc tgaacgcaat cgctgcgttg cgggtgctgg tctcggttga
     4981 tttctcccca agaggacgat tcgatctggc caaacggcag ttgaacagcg tgatgcgatc
     5041 agccctcnnn nnnnnnnact tataaactct gcggatgagc agatccgcgt cggcacatcg
     5101 atccgaaacc ctgaaggcac tgtacccact gctctttgtc gcactatgga gcactggctt
     5161 tatcggtgcc aagttcggtc tgccgtatgc cgaaccgctc actttcctga ccattcgtta
     5221 ccttgccgtg ctggttctga tgggagcaat cacctggcgc atgcgtgcgc cttggccgcg
     5281 aactgggctg gaatggattc acattggtat ttcaggactc ctcatccatg cgctctacct
     5341 gggtggcgtg ttcaccgcca tcagccacgg cttgcccgca ggagtcacct ccctggtggt
     5401 gggtttgcaa ccggtgctca cggctctggg tgcagggcta ctgcttggcg agcgcgtgcg
     5461 ccccacccag tgggcaggat tagcgttggg ttttgtgggt gttggattgg tggtggcgca
     5521 caaggtggtg gccagcgcaa gcggtggcgc gctgttggtc atgttgctgc ctgccgctat
     5581 cgcgctgctg ggcatcaccg tcggcacgtt ataccaaaag cggttttgcc aagcattcga
     5641 cctacgcacg gggtcggtga ttcagtttat gccgtgcctg gcggtcactg cgctggtagc
     5701 cagccagacg gaaaccatgc aggttcaatg ggccgactcc ttcatgttcg cactcggatg
     5761 gttggtactg gtgctctcgc tgggtgctgt gagcttgctg aacgtcttga tccgcagcgg
     5821 cagtgccgtg aacgtcgcca gtttgttcta tctgacccca cccacaactg cactcattgc
     5881 ttgggcgctg ttcgatgaga cccttactgg cttggccttg atcggcatgg ggctgacggt
     5941 gttcggcgtg tggatggcgc gaaagtgaaa tttgtgtgat gttgggaaaa ccggcaatga
     6001 ggtgttctca acagtttcgc cattcgtctc gctttgggtt cgcggagccg ggtcgtccct
     6061 aacgtaaagg acgactgcat ttgcagccac agtcaattct gtagtaccgc cccgctgctc
     6121 atgcgtggcg agcccagcag tctgataagc gctcagcaac agtcggttgg tttgggcagc
     6181 acaaagcgct gaaccgcaaa aaaccctcaa agcttcaaac gaggttgtgc gatggcactg
     6241 gttcgtcacg cctgaccgtt cacgcaccgc cgtagtcaat gccattttga tgcccaagcg
     6301 ggtctcaccg acaaacctgc atcgaacgtt gtgcgccgtc gatggcatat ctcctcggga
     6361 agcgcccatc gactgtcgtc acactcgaac cgctacaccc ccaagcagca tgtgcgggta
     6421 tgacaaacga ggcttgcacc accttctgcc tatacgcatg ggcacaccgt cactgatagg
     6481 gacaccgtta acaaatattg gcaaattaac aattgaatac aaatgctatt gcgtttttac
     6541 gatcaatggc cgaaaatgcg tcatggtttc aagtttgaca accccacagg acctaaacct
     6601 tagcgtcgca gagactgttg atttgctgac caaagaactc ggcggtgagt catcccgatg
     6661 ggcaa
//
LOCUS       DAOSLL010000203         6663 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10435_length_6663_cov_3.692494, whole genome shotgun sequence.
ACCESSION   DAOSLL010000203 DAOSLL010000000
VERSION     DAOSLL010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10435_length_6663_cov_3.692494"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1699
                     /locus_tag="PLB25_10135"
     CDS             <1..1699
                     /locus_tag="PLB25_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE41972.1"
                     /translation="RHLLENTAAGDVVLMAYMDVDQLKAINDSVGQQVADQLLVTLAN
                     RLREASRRHDLLARLSGDEFALVMSGGGDTELMQGVAERLLRGVSHGAVIDGCAVNFS
                     LSVGTALFPADASDCNELVSNAETALLAAKRESGGAFRRYQSGLTQSGKNRSEAVDAL
                     RQAIGNNELVLHYQPQLSLHSGQIIGLEALVRWQHQTRGLLAPGLFLPLAEESGLIIP
                     LGEWVMRRAVAQVRSWLDEGLSAVRVAVNLSARHFRQRELPEAIENLLREYAVPAHLF
                     ELELTESVMMHDAETAIRIVERLKALGLRISLDDFGTGYSSLAYLSRFAIDVIKIDQS
                     FVNDITSNPVNASIAIATIAMAHKLGKTVIAEGVETEGQMLYLRRHECDEMQGYFFSR
                     PVPAQAISDMLRQGAGLNFGPAGKEEQLTLLLVDDEPSILNALKRLLRREGYRILCAE
                     SGAQGLELLATNSVQVIVSDQRMPTMTGTEFLSRVKDLYPDTLRLVLSGYSELNTLTD
                     AINKGAIYRFLSKPWDDEKLKQEVQQAFRHYRETRQPTVSPDRLDSVDGAAPLKGSR"
     gene            1696..4908
                     /locus_tag="PLB25_10140"
     CDS             1696..4908
                     /locus_tag="PLB25_10140"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE41973.1"
                     /translation="MTASTEPMDLPTRSNWWLLASVLVVAGLAFGLNIWNNYESTDLE
                     ARELLKLQAKNVGDDLGRHLKVIDRVLTDVRANMPHRTAQGNGRQITEQHLKVVARAM
                     EGVRTLTVFDAAGTITLSSRPEVTGQNFADRAYFQMARRETNPAALNLSAPFQTVLGV
                     NSMIAIKSIIGPDGKFAGAVSVALEPDDFKSLLEGVRTTADLVVGIVHGEGKVFVFSP
                     ESASPPNANTAQPGTFFTRHMESGQNTSVMTGWSKTMNSERLLAAHTVKPDSLVMNQS
                     LLVSVSRNLPLVYASWRKQSYELIVLFGAVALISTVSLYLHQRRHRVALDVEHKLHRS
                     DTQFTAFFDSAMVGMAVTSLEKSWIRVNPALCDILGYSREILSQKTWADLTHPEDLAA
                     DVAEFERLLSGESNEYSMEKRFIRSSGEIVHAFIAVRAVRNASNDIDFFAAIVENITD
                     RRKAEQQTRSTLHMMQRFIDCMPGLVYIKDEDSRIVMANQGFAHFGFDKHALIGKTTL
                     EIIPGPIGEKIARDEERIQQSGKSEVMIDELRGSFYESSRFAIDGEDGQRLLGGITLD
                     VTQRQRYVERAQALLLIDSLGGKMPEKEFLTQGLELAERLTGSGIAFLHFVNEDQQTI
                     ELVTWSSKALKGCTAVHESHYPIGQAGIWADCFRQKQAVTFNDYPSYLAKKGLPEGHA
                     PLQRLISVPVIEEDQVRIMLGMGNKRDDYDDFDIDTVQLIGNDLWRIARRVRVEAMLQ
                     QKISELTLLNQRLSEAQGQLLQSEKLAAIGQLAAGVAHEINNPIGFVRSNLGTLADYV
                     DDLLSIDRVYMDFEDQLAAREPTLFARVKAIKNECDHDFIVGDLKNLLHESGEGLDRV
                     IKIVQDLKGFSRIGESGWLWADLHMGLESTLNIVHNEVKYKAEVVRELGDLPEVYCIP
                     SQINQVFMNLLLNAAQAIESHGVITLRSGCDGAWVWIEIRDNGCGIAPATVGRIFEPF
                     FTTKPVGKGTGLGLSLAWGIVQRHLGTIDVQSEVGKGTTFRIRLPIAGPGANQAKCAH
                     PPKFRHFVKTSQSEVLWTTEFALDINHLACQRHLSFAVLA"
     gene            complement(4859..5263)
                     /locus_tag="PLB25_10145"
                     /pseudo
     CDS             complement(4859..5263)
                     /locus_tag="PLB25_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(5273..5668)
                     /locus_tag="PLB25_10150"
                     /pseudo
     CDS             complement(5273..5668)
                     /locus_tag="PLB25_10150"
                     /inference="COORDINATES: protein motif:HMM:NF040080.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6398 domain-containing protein"
     gene            complement(6439..>6663)
                     /locus_tag="PLB25_10155"
     CDS             complement(6439..>6663)
                     /locus_tag="PLB25_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41974.1"
                     /translation="FAASRLSFLTPESQERSFSGENHLFKLAVGTAAVADGRGQPWNF
                     IDFSKAVIADVVPISSSLHLQTSKADIRGK"
BASE COUNT         1461 a         1767 c         1988 g         1447 t
ORIGIN      
        1 gcgccacctg cttgaaaata cggctgcggg tgatgtggtg ttgatggctt acatggacgt
       61 tgaccagctc aaggccatta atgattcagt ggggcagcag gtggctgatc agctcctggt
      121 cacgctggcc aatcggctgc gtgaagcctc gcgccgccat gacttgctgg cgcgcctgag
      181 tggcgacgaa tttgccctgg tcatgtcggg tggcggtgac actgagctga tgcaaggcgt
      241 ggccgagcgc ctgttgcgcg gcgttagcca tggcgcggtg atcgatggtt gtgccgtaaa
      301 tttttcgctc tcagtcggta ccgccctgtt tcccgccgat gcatcggact gcaatgaact
      361 tgtgagcaac gcagaaaccg ccctgctggc ggccaagcgg gagtcgggcg gcgcctttcg
      421 gcgttatcaa tcgggcctca cccaatcagg caaaaatcgg tccgaggctg tggatgcatt
      481 gcgccaggcc atcggcaaca acgagctggt attgcactac caaccccagt tgagccttca
      541 ctcggggcaa atcattgggc tggaggcctt ggtacgttgg cagcaccaaa cccgcgggtt
      601 gctggcgccg gggcttttcc ttcccttggc cgaagaaagc ggcctgatca tcccgcttgg
      661 cgaatgggtc atgcgccggg cggtggcgca agtgcgaagc tggctggacg agggcctgtc
      721 agcggtgcgg gttgccgtca atttgtcagc ccgacatttc cgacagcggg agctgcccga
      781 ggcaattgaa aacctgttgc gggaatacgc agttcctgcg catctgtttg agttggagct
      841 gaccgagagc gtcatgatgc atgacgctga aaccgccatc cggattgtcg agcggctcaa
      901 agcgctgggc ctgcgcattt cgctggatga ttttggcacc ggttattcgt cgctggccta
      961 tttgtcgcgg tttgccattg atgtcatcaa aattgaccag tcgtttgtga acgacatcac
     1021 aagcaaccct gttaacgcat cgatcgccat cgccaccatc gccatggccc acaaactggg
     1081 taaaaccgtt attgccgaag gggtcgaaac cgaggggcaa atgctttact tgcgccgcca
     1141 tgaatgcgac gagatgcagg gctacttttt ttcgcgcccg gtaccggccc aagccatcag
     1201 tgacatgctg cgccagggtg ctggcctgaa ctttggaccg gcgggcaaag aagaacaact
     1261 gaccctgctg ctggtggatg atgaacccag catcctcaat gcccttaagc gcttgttgcg
     1321 ccgggaaggg tatcgcatac tgtgcgcaga gagtggggct cagggcctgg aactgcttgc
     1381 gaccaactcg gtacaggtca tcgtgtccga tcagcgcatg ccgaccatga ctggcaccga
     1441 gttcttgagc cgggtcaagg acctttatcc tgacacattg cggctggtgc tgtcggggta
     1501 ttccgagctg aacacgctga ccgatgccat caacaaaggc gccatttatc gcttcctgtc
     1561 caaaccctgg gacgatgaaa agctcaaaca ggaagtgcag caggcgtttc gccactaccg
     1621 tgagacacgc cagcccacgg tgtcgcccga ccgacttgat tctgttgacg gcgcagcgcc
     1681 cctcaaaggc tcgagatgac agcatccacc gaacccatgg acttgcccac ccgctcgaac
     1741 tggtggctgc tggcaagcgt gctggtcgtc gcaggcttgg ctttcggcct gaacatctgg
     1801 aacaactacg aaagcactga cctggaagcc cgcgaattgc tgaagttgca ggcaaaaaac
     1861 gttggagatg atctggggcg ccatctgaag gtgattgacc gtgtcttgac agacgttcgc
     1921 gcgaacatgc cgcaccggac cgcccagggc aatggccggc aaataaccga acaacacctc
     1981 aaggtggtcg cccgtgcgat ggaaggggtc aggacgctga cggtgtttga tgccgctggc
     2041 accatcacgc tcagcagccg cccggaggtc accgggcaga attttgcgga ccgggcgtat
     2101 ttccagatgg cacggcgtga aaccaacccg gccgcgctga acctgagcgc cccgttccag
     2161 acggtgctgg gggtcaattc catgattgcg atcaaaagca tcatcgggcc cgatggcaag
     2221 tttgcgggtg ccgtgtctgt tgccctcgag ccggatgatt tcaagtccct gctcgaaggg
     2281 gtacgcacta ctgcggatct cgtcgtgggc attgtccatg gcgaaggcaa ggtgttcgtc
     2341 ttttcaccgg agtccgcaag cccgcccaac gccaataccg cccagcccgg aacttttttc
     2401 acccgtcaca tggaaagtgg acaaaacacc agcgtgatga caggttggag caaaacgatg
     2461 aacagcgaac ggctgctggc ggcgcacacg gtaaagcctg actccctggt gatgaaccag
     2521 agcctgctgg tgtccgtaag ccgaaatttg cctttggtgt atgccagctg gcgtaagcag
     2581 tcatatgaac tgatcgtgtt gtttggtgcg gttgcgctga tcagcacggt cagtctgtat
     2641 ctgcaccaac ggcgtcatcg ggtggcactc gacgtggaac acaaattgca tcgaagtgac
     2701 acccaattca cggcgttctt tgacagtgcc atggtgggca tggccgtgac ctcactggaa
     2761 aaaagctgga tccgggtcaa tccggcactc tgcgacatac tgggttattc cagagagatt
     2821 ttgagccaga agacttgggc cgaccttacc cacccggaag atctggctgc ggatgttgcc
     2881 gagtttgaac gtctgctgag tggcgaaagt aacgaataca gcatggagaa gcgattcatt
     2941 cgctccagcg gcgaaatcgt gcatgccttt attgcggtgc gcgccgttcg caatgccagc
     3001 aacgacatcg attttttcgc agccattgtg gaaaacatca ctgaccgacg caaagccgaa
     3061 cagcaaacac gcagcaccct gcacatgatg cagcgcttta tcgactgcat gccggggctg
     3121 gtctatatca aggatgaaga ttcgcgaatt gtgatggcca accagggttt tgcccacttt
     3181 ggctttgaca agcatgcttt gatcggcaag accactctgg aaatcattcc aggaccgatc
     3241 ggcgaaaaga ttgcccgcga cgaggagcgc atacagcaaa gcggcaaatc cgaagtgatg
     3301 atagatgagt tgcgcggcag cttctatgag tcaagccgct tcgccattga tggagaagac
     3361 ggccaacgcc tgctcggagg aataacactc gatgtaaccc agcggcagcg gtacgtggag
     3421 cgtgcgcagg cattgctttt gatcgattcg ctgggcggca agatgccaga gaaagaattc
     3481 ttgacacagg ggctggaact ggccgaacgg ctcaccggca gcggcattgc ctttttacat
     3541 tttgtcaatg aggaccagca aaccattgag ctggtgacct ggtccagcaa ggcactgaag
     3601 ggttgcactg ctgttcatga gtcgcactac ccgattggac aggcaggcat ctgggccgac
     3661 tgcttcaggc aaaaacaggc cgtgactttt aacgattacc ccagctatct cgctaaaaag
     3721 gggctacccg aggggcacgc gccattgcag aggctgatat ccgtacccgt gattgaagaa
     3781 gaccaggtgc gcatcatgct gggcatgggc aacaagcgcg acgactatga cgactttgat
     3841 atagataccg ttcaattgat tggcaatgac ctgtggcgca ttgcccgacg ggttcgcgtc
     3901 gaggccatgt tgcagcaaaa aatcagcgaa ctcacgctgc tcaatcaaag actgtccgaa
     3961 gcgcaagggc agttgcttca atctgaaaag ctcgccgcca tcgggcaact cgcggctggc
     4021 gtggcgcacg agatcaataa cccgatcggg tttgtcaggt ccaatttggg cactttggcg
     4081 gactatgtgg acgatctttt gtccattgac cgggtctaca tggactttga agaccagctg
     4141 gcggcgcggg aacccacact ctttgcgcgt gtcaaggcca tcaagaacga gtgcgatcat
     4201 gacttcatcg tgggggactt gaagaatttg ctgcacgaat ctggcgaagg gctggatcgg
     4261 gtcatcaaaa tcgtgcagga cctgaaaggt ttttcacgga ttggcgaatc cggctggttg
     4321 tgggcagatt tgcatatggg gctggagagc acactcaata tcgtgcacaa cgaagtcaaa
     4381 tacaaagcag aagtggtgcg ggagctggga gatttgcccg aggtttactg cattccttcg
     4441 cagatcaatc aggtgttcat gaatttgctg ttgaatgcgg cgcaggcgat tgaatcccat
     4501 ggtgtgatca ccctgcgctc gggctgcgat ggtgcatggg tgtggatcga gatcagggac
     4561 aacggctgcg gcattgcgcc cgccacggtg ggccgcattt tcgagccctt ttttacgacc
     4621 aaaccggtag gcaaaggcac cgggctgggc ctgtcactgg cgtggggcat cgtgcaacgc
     4681 cacctgggta cgattgacgt gcaatcggaa gtgggcaagg gaacgacctt cagaattcgc
     4741 ctcccgattg cggggcctgg cgcaaaccag gctaaatgtg cccatccccc gaaattcaga
     4801 cactttgtaa agacatcgca aagcgaagtg ctgtggacga cggaattcgc tctggacatc
     4861 aaccatttgg cgtgccaaag gcatctctca tttgccgtgc tcgcttgacc tgatcggtgt
     4921 gaaggtacac cgacgtggtg gacaccgagg catgccgcag gttgtctctc acagtggtca
     4981 attctgctcc ccgcgccaaa gcgtgtgtgg cgtgggtgtg cctcatccag tgcggacttg
     5041 ctcttcgcaa cttctcggcc gtttttgggg actcagcctc gatggcatca gcagttgtta
     5101 tgaagaaacg gttgatgatg tcccacaacc ggctagaagt aatgtggctg tttccgccgc
     5161 cgttctgcaa atctgcgatg agcgcagtgg acggcttcca catggctggc gtagttggta
     5221 caccgcgctg catcaagtag tgatccagag ccacctttgc caaggtccag ttcggatcaa
     5281 gtgggtaaat cttcagcatc tttcggattg ccgccagttt cgctgccccg ttgcttgggc
     5341 tggttccaag aaggtggtcg atgtcagttg agcgcatgta aggtgtctgg ctcttgtcgt
     5401 gcaagaagtt gaccccgcct atggcccgaa ctatggcgct tgcccaggca ttcgggctgc
     5461 catggagcag gggcgagggt cgctttcggg ccagtttctc ggccaacttg cggcaaagta
     5521 tggcgtactc ttcggtcaaa tgttcattgc aaaacgggtt gattaatccg aggacgacgt
     5581 ttagggttcg gtccttttcc tcccttgatg gcggcgtcgg gagcggtttg gctttcggcg
     5641 ctttcggtgc caaggacgtg tttgacattg gcaacgacgt gacaatcccg tgtcgctcct
     5701 tggcctcctt gaaatcctgg tttaaatttc gccgtatccc agggccaaac gagatgttgt
     5761 agccgctcat caagccgtcg tagacgatca tgtttttaaa gggcaacaag acagtttgaa
     5821 tcagcaccgg caagtgtggc ccagtcaagt cctcaaatgg ttgggacaat gccatgacgc
     5881 cgtaggcaat ggctggggag cttgcggaca ggaacactgt gtagttcttg agttcccgga
     5941 aaacgtagaa cctgccggcc acaagatgtc gccaagaacg aacgacgtcg agctcgcctt
     6001 ctgtgaagtg ggccggattt tcagcgacaa aggactcgat cagatcgagg tttgcattca
     6061 aagcatcacg ggctttggcc cgaacttgcg gcgacagggc ggcgaactcc tgcgccgtcg
     6121 tgacactctc tgaatcaata ttgaggcgct ggctcacgaa aaacatgagc gcccggtgca
     6181 atttgaagaa ctggtcgaga tcgggtggag ttaacagcat ttggaaggag ccattttggg
     6241 ggctggacgg ataggggatg tcccacatat tgtcgctgcc ggttcagtcc tgcccaaaac
     6301 gaacgttccg gcttctacgc atgttcacca tctttaacaa gccgggggaa tgtggtgaat
     6361 gtcgggtatt gggctagcat tttggccaga cctatgtcag cggcagatgc agtaccgaca
     6421 gccactggca aaaatcgatc atttccctcg tatgtccgct ttcgaggttt ggaggtgaag
     6481 agatgacgaa attggaacca cgtcagcgat gaccgctttg gagaaatcga tgaaattcca
     6541 gggttgaccg cgaccgtccg caaccgctgc agtgccgaca gccagtttga aaaggtgatt
     6601 ttcaccgctg aatgaccgct cctgagattc gggtgtcaaa aacgaaagtc tgcttgcggc
     6661 gaa
//
LOCUS       DAOSLL010000204         6646 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10481_length_6646_cov_4.672432, whole genome shotgun sequence.
ACCESSION   DAOSLL010000204 DAOSLL010000000
VERSION     DAOSLL010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6646)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6646)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6646
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10481_length_6646_cov_4.672432"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="PLB25_10160"
     CDS             <1..1048
                     /locus_tag="PLB25_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOE41975.1"
                     /translation="SNLPKWLVGLGWMERAARGPRPPMPDDPVRQFFASGRGLAESIG
                     EWAFYAAAALLLLALIRRFPYKYFFKTHRLLAVAYLALVWHSVVLLKFDDWSGLLGPV
                     MLVLMGAGSVAAVMVLSGRVASRRQTVGEVVGIRRHDALQVTELDIALQGRWAGHEAG
                     QFAFVTLHAQEGAHPYTISSAWANDGRLTFIIKALGDYTGTLAQRLRLKDVVKVEGPY
                     GRFNFEGEKKRQIWVGAGIGITPFIARMKTLAKSPDGRSVHLFHSTRDFDADAISLLE
                     SDAKAANIGLTVLVDARDGQLNAARIAAQVSDWRDADIWFCGPSRFGQSLKNDFVAMG
                     LSQQQFHQELFEMR"
     gene            complement(1183..3456)
                     /locus_tag="PLB25_10165"
     CDS             complement(1183..3456)
                     /locus_tag="PLB25_10165"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOE41976.1"
                     /translation="MSVFRLRCVACMLGLLLAVGAAGASTVTQTDAQTLARAVALRNG
                     GSLQQAIEIFSALRTGSDAPTGLAATGELGTTLLQARRYDEAEPLLRTAYDKTQGIQR
                     ARYAIELGNLAAARRQVPAALLLYVEARSLAGNDGALTLVARINQARLAERQERPAFL
                     AAISHDLASIGEPMARAQARLSVGTLAGEIGSMQIAYNNLEEARTLAAQVGNARIEIE
                     ALDGLAQLYEAQGRLADALLLSRRGSALAQAAPGVLTDISLRLEWRQGRLLVLQGAND
                     EAQAAYQRAIAHIESIRQDIPIEYEGGRSSFRDTLEPIYLGLIDLLLKKADKAQGSEQ
                     TSLLRHARDMAELIKQSELQDYLGERCSVDSAQSGAQSGLAAGTAIVYPIILKDRIEM
                     IFEAGNGITRHTSPMDAQSLRNLVLSHTGNLRRQESAYLEGASQLYDLLVRPFEKDLQ
                     TQGIHTLLVVPDGVLRLLPWATLFDGKQFLVENFAVVINSGLSMTSQAADRTQGFSAL
                     VAGMAEPGPVVEKLALRLSGNAADAAAMQSGVAAGLTQSRATRAISPTMRTASVDTPE
                     RFAALKKSLVLPGVKAEVDALSIILPGTRLLDATFTTSRLHDEFDKGTYRIAHLATHG
                     FFGSTGKQSFIMAYDDLVTMDGLQELLQLEGARKIPIELLTLSACETAEGDDRSPLGI
                     AGAAIKAKARSVLGTLWPVADDAAQQVMTKFYSSLVSDGTSKAEALRLAQRALITDRE
                     LDHPFFWGPFIIIGNWR"
     gene            complement(3453..5135)
                     /locus_tag="PLB25_10170"
     CDS             complement(3453..5135)
                     /locus_tag="PLB25_10170"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HOE41977.1"
                     /translation="MQKAPLIGLATLLAAGFALAIEPDHLNQQTPPLPPYLKPAPEEN
                     FALPPVAPDAAVIMPSAQNVVLDRVLFVGNSVVSTQDLQAVAAPLIGKVVGAADIETL
                     RQQASLLYVKRGYVNSGVVLAADAFKQRTLTLRVIEGRLKEIRLRGLDGLNEHYVADR
                     LAQGDEPFNLQTLGERFQLLLTDPLFDRMNAQLLPDTERGKAILNVDIDRALPYQLSL
                     SANNYRPVSIGAENATLSGWVRNLTGRGDILEASYQSPLGDGSARRSALGWRMPLNTW
                     GTQLSVNYDQGSSSVIEQPLEPIDIQSQLFNHDIGISQTLFETLRHKLVVGINRVGRE
                     DRTTLLGVPFSFTPGEPTGTSEATDWRFWQEYSHRWDKQVLALRSTFTKGENNQTDIG
                     TVPGATPDRRYCVWLGQAQYAQRLFDKDTQLIVRLNVQSSPQRLLAMEGMSVGGISTV
                     RGYRENQMVRDNGAVANVELDFPLIADGANKLHLNLRPFFDYGRAWNKGEEKSAISSV
                     GLVARLRWQRWALDLALAKRRSHPDSVTSSGSNLQDKGIHIQVSYNFFGAGQ"
     gene            complement(5136..>6646)
                     /locus_tag="PLB25_10175"
     CDS             complement(5136..>6646)
                     /locus_tag="PLB25_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41978.1"
                     /translation="ASIESNSIGNTDNGGKAGDAGSISITASGALSIANGGTISSSTS
                     SSGMAGTVKVSAGSIAIDGQGNTTGIASQALGEADNAGKTGDAGSIDITASGALLIVN
                     GGGISSTTFSFGNAGTVKVSAGSITIDRQDSNWAGIASQAIGDATNGGKTGDAGNLDI
                     AASAALSVINGGKISSGTFSSGKAGTVKISAGTLLVDGKFSSINAKADAGSSGQTGSV
                     SVTALDGITLSNQGTLSIQNDATLAQPGTLTPTLLTVSAPIIAISGGQISAASTGNVP
                     AGSIQVVGSKLLLENGSSIATSSNLGNGGPIDIAAAALWLNNSQITTSVAGRTGNGGN
                     ISIAADVLAMNTGFIQANTVAANASGGLVSVTAQNLIPSGTSLILGGNTPYSVQPGVF
                     GYNVIQAAAPTGISGVIQVSAPTLDAAASLLGLNTGLMRNDQLARHPCDNIGGSSLVP
                     VGRGGLPPGANDFLSPGQFSASFIATTIGKTGHSAIGSSLPLAAYSPCQGAL"
BASE COUNT         1259 a         1987 c         2042 g         1358 t
ORIGIN      
        1 ttccaatttg cccaaatggc tggtcggtct gggctggatg gagcgcgcag cacgtggccc
       61 gcgcccacca atgcccgatg acccggtgcg ccaatttttt gccagcggcc ggggcctggc
      121 tgagagcatt ggcgaatggg cgttttatgc ggcggcagcg ctgctgctgt tggccttgat
      181 caggcgcttt ccgtacaaat actttttcaa gacccaccgc ttgctggcgg tggcctacct
      241 ggcgctggta tggcattcgg tggtcttgct gaaatttgac gactggagcg gcctgctcgg
      301 gccggtcatg ctggtgctga tgggcgcggg cagcgtggcg gctgtgatgg tgttgtcggg
      361 gcgggtcgca agccggcggc aaactgtcgg cgaggtggtg ggcattcgcc gccatgatgc
      421 cttgcaggtg accgagcttg acattgcatt gcagggccgc tgggccggtc acgaggcggg
      481 gcagttcgcc tttgtcaccc tgcatgcaca agagggtgcg cacccttaca ccatctcatc
      541 ggcctgggcc aatgacggtc ggctgacgtt catcatcaaa gcccttggcg actacaccgg
      601 caccctggcg cagcgcctgc ggctcaagga tgtggtcaag gtcgaagggc catacggtcg
      661 cttcaatttc gaaggcgaaa aaaagcgcca gatctgggtt ggcgctggca ttggcatcac
      721 gcccttcatc gcccgcatga agaccctggc caagtcaccc gatggcagaa gcgtgcacct
      781 gtttcacagc acccgcgatt tcgacgccga tgccatcagc ctgcttgaga gcgatgccaa
      841 agccgcaaac attggcctga cggtgctggt ggatgcgcgt gacggccagc tcaacgcggc
      901 acgcattgcg gcccaggtat cagactggcg cgatgccgat atctggtttt gtggtccgtc
      961 acggttcggc caatcgctga aaaatgactt tgtcgccatg gggctgtcgc aacagcaatt
     1021 tcaccaggag ttgtttgaga tgcgctgagc tgccgccggg tttgatgcac aaaggggcca
     1081 ggttcgaatg aatatgcttt gaccgctctc gaattgggta ccgacggcta ctggcaggga
     1141 acacccggta acggttgcca taccgcaaat gttcaaccgc ctttagcgcc aattcccgat
     1201 gatgataaac ggaccccaaa agaacgggtg atcgagctcc cggtccgtaa tcagcgcccg
     1261 ttgcgcaagg cgcagtgcct ctgccttgga tgtgccatcg ctgaccaagc tgctgtagaa
     1321 cttcgtcatc acctgctgcg ctgcatcatc agccaccggc cacagggtgc caagcacgct
     1381 tctggccttg gctttgatgg ctgccccggc aatgcccagc ggagagcgat catcgccttc
     1441 ggctgtttcg caggccgaca gggtcagcag ctcaatcggg atcttcctgg ccccttcgag
     1501 ttgcaacagt tcttgcaaac cgtccatggt caccagatca tcgtaggcca tgatgaagct
     1561 ctgtttgcca gtgctgccaa agaacccatg ggtggccaga tgcgcgatgc ggtaggtgcc
     1621 cttgtcaaac tcatcgtgca aacggctggt ggtaaaggtg gcatcaagca gccgggtgcc
     1681 gggcaaaatg atcgacaaag catcgacttc cgccttgaca ccgggcaaca ccaggctttt
     1741 cttcaaggcc gcaaaacgct ccggggtgtc tacgcttgcc gtgcgcatcg tgggcgatat
     1801 ggcacgcgtt gcccggctct gggtcagccc ggcggcaacg cctgattgca tggcagcggc
     1861 gtcggcagca tttccggaca gcctgagcgc cagcttttct accaccggac ctggctccgc
     1921 catgccagcc accagcgcgc taaacccttg cgttcgatcc gccgcctgac tggtcatcga
     1981 cagaccgcta ttgattacca ctgcaaagtt ttcgaccaaa aactgcttgc catcaaagag
     2041 cgttgcccat ggcagcaaac gcaaaacgcc gtcaggcacc accagcaagg tgtgtatgcc
     2101 ctgggtctgc aggtcttttt cgaaaggccg gaccagcaag tcgtagagtt ggctggcccc
     2161 ctcaagatag gccgactcct gacggcgcag gttgccggta tgggacaaca ccaggttgcg
     2221 cagtgactgg gcatccatcg ggctggtgtg gcgcgtaatg ccgttacccg cctcaaaaat
     2281 catctcgatg cggtctttca ggatgattgg ataaacaatc gcggtacctg ctgccaagcc
     2341 tgattgcgca ccgctttgcg ccgaatcaac gctgcaacgc tcacccagat aatcctgcag
     2401 ttcggattgc ttgataagtt cggccatatc gcgggcatgg cgcaacaggc tggtctgctc
     2461 gctgccttga gccttgtcgg ccttcttgag cagcaggtcg atcaagccga gatagatcgg
     2521 ctccagcgtg tcccgaaacg aggaccgccc gccctcgtat tcaattggaa tatcctggcg
     2581 gatgctttcg atgtgcgcaa tcgcccgctg gtaggctgcc tgcgcttcat cattggcacc
     2641 ttgcagcacc agcaggcggc cctgtcgcca ttcaagacga agcgagatat ccgttagcac
     2701 gcccggtgcc gcctgtgcca gtgcactgcc acgccgactc agcagcagcg cgtcggccaa
     2761 gcgaccctgt gcttcgtaaa gctgtgccag gccgtccagt gcttcaattt cgatgcgggc
     2821 gttgccgacc tgcgcggcca gtgtgcgtgc ttcctcaaga ttgttgtacg cgatttgcat
     2881 actgccgatc tcgcctgcca gtgtgccgac cgatagccgg gcctgcgccc gcgccattgg
     2941 ctcgccgatg ctggcgagat catgcgagat cgctgccagg aaggctggcc gctcctggcg
     3001 ctcggccagg cgggcctgat tgatgcgggc gaccagggtc aatgcgccgt cgttgcctgc
     3061 cagggatcgt gcctccacat agagcagcaa agccgctggc acttgtcgcc gcgcggcagc
     3121 caggttgccc agctcaatgg cataacgtgc ccgctgaatg ccttgggttt tgtcgtaagc
     3181 cgtgcgcaac agtggctcgg cctcgtcgta acgccgcgcc tgcagcaaag ttgttcccaa
     3241 ttcgccggtg gcagccaggc cagtgggcgc atcgctgcca gtgcgcaggg cgctgaaaat
     3301 ttcgatggcc tgctgcaaac tgccgccgtt acgcagcgct acggcgcgtg ccaatgtttg
     3361 ggcatctgtt tgggtgactg tgctggcacc ggccgcgcca acggccagca gcagccccag
     3421 catgcaggcg acacaacgca atcgaaagac gctcattggc ccgccccgaa aaagttgtaa
     3481 gacacctgaa tatggatgcc cttgtcctga agattggacc cgctgctggt gacggaatcc
     3541 gggtgcgatc ggcgtttggc cagcgccaga tcaagcgccc agcgctgcca gcgcagccgg
     3601 gcgaccaggc cgacggagga aatggccgat ttctcctcac ccttgttcca ggcccggcca
     3661 tagtcaaaaa agggccgcag attgaggtgc aacttgttgg caccatctgc tatcagtgga
     3721 aaatccagct cgacattggc cactgccccg ttgtcgcgca ccatctggtt ttcccggtag
     3781 ccgcgcacgg tgctgatgcc gcccaccgac atgccttcca tcgccagcag ccgctgcggc
     3841 gagctttgga cattcaggcg aacaatcaac tgggtgtctt tgtcaaacag ccgctgggca
     3901 tactgggcct gcccgagcca gacacagtag cggcgatcag gtgtcgcgcc cggcaccgtg
     3961 ccgatgtcag tctggttgtt ttcgcccttg gtaaaggttg atcgcaaggc cagcacctgc
     4021 ttgtcccagc ggtgcgaata ttcctgccaa aagcgccagt cggtggcctc ggatgtgccg
     4081 gtaggctcgc ccggcgtgaa agaaaacggc accccgagca gcgtggtgcg gtcctcgcgc
     4141 ccgacccggt tgatgccgac gaccagcttg tgccgcagcg tctcgaacag ggtctggctg
     4201 atgccgatgt cgtggttgaa caattggctc tggatgtcaa tcggctcaag cggctgttcg
     4261 atcaccgatg agctgccttg gtcgtaattg accgacaact gggtgcccca ggtattgagt
     4321 ggcatccgcc agcccagcgc gctgcgcctg gcagagccgt cacccagcgg gctttgatag
     4381 cttgcctcca ggatgtcacc acgaccggtc aggttgcgca cccagccgct cagcgtggcg
     4441 ttttcggcac cgatggatac cgggcgatag ttgttggccg acaggctcaa ctggtagggg
     4501 agcgcccggt cgatatcgac gttgaggatc gccttgccac gttcggtgtc gggcagcagt
     4561 tgtgcgttca tgcggtcaaa cagggggtcg gtcagcagca actggaaacg ctcgcccagc
     4621 gtctggaggt tgaacggttc gtcgccctgc gccagacggt ctgcgacata gtgctcgttg
     4681 agcccgtcaa ggccgcgcag ccggatctcc ttgagtcggc cctcgatgac ccgcagcgtc
     4741 agcgtgcgtt gcttgaacgc atcggctgcc agcaccacac cagaattgac atagccgcgt
     4801 ttgacgtaca gcaggctggc ctgttggcgc agtgtctcaa tgtctgcggc accgacgacc
     4861 ttgccaatca gcggggctgc tacggcctgc aagtcttgcg tgctgacaac gctgttgccg
     4921 acaaaaagca cccggtcaag caccacgttt tgcgcacttg gcatgatgac agcggcatcg
     4981 ggcgcgaccg gtggcagcgc aaagttctct tcaggcgcgg gcttgagata aggcggcagc
     5041 ggtggtgtct gctggttcag atgatcgggc tcaatcgcca gtgcgaaacc ggctgcgagc
     5101 agcgtggcca acccaattaa tggcgctttt tgcatctaca aggctccctg acaaggcgaa
     5161 taagctgcca ggggcaagga tgagccaatg gcggaatggc cggtcttgcc aatcgttgtg
     5221 gcgatgaaac tcgcactgaa ctggccgggg ctgagaaaat cgttagcacc tggtggcaaa
     5281 ccgccgcgtc caaccggtac cagcgaactg ccgccgatgt tgtcgcaggg gtgtcgcgca
     5341 agctgatcat tcctcatcaa accggtgttc agacccagca ggctggccgc tgcatcgagg
     5401 gttggtgccg acacctgaat cacaccgctg atgccggttg gtgcggccgc ctggatgacg
     5461 ttgtagccga aaacgccggg ctgaacgctg tagggcgtgt tgccaccgag tatcaggctg
     5521 gtgccgctgg gaatcaggtt ttgcgccgta accgacacca ggccaccgct ggcgttggcc
     5581 gcgacggtat tggcctggat gaaaccggtg ttcatggcca gcacgtccgc cgcaatgctg
     5641 atgttgccac cattgccagt ccgtccggcc acagatgtgg tgatctggga attgttgagc
     5701 cacaacgcgg ctgccgcaat gtcaatcggg ccaccattgc ccaggttgga cgaggtggcg
     5761 atgctgctgc cgttttccag caacagcttg ctgccaacaa cttgaatgct gccggcaggc
     5821 acattaccgg tcgaggcggc gctgatctgg ccaccactta tggcgatgat tggcgctgaa
     5881 accgtcagca gggtgggtgt cagggtgccg ggttgggcca gggtggcatc gttctggatc
     5941 gacagggtgc cttgattgct gagtgtgatg ccgtcaagcg cggtaacgct gacactgccg
     6001 gtctggccag atgacccagc atctgctttg gcattgatgc tgctgaattt accgtcaacc
     6061 aacagtgttc ctgcactgat tttgaccgtg ccagccttgc cagaggagaa ggtaccagaa
     6121 gaaatttttc caccattgat gacggaaagc gcagcgctgg cggcgatgtc aagattgccg
     6181 gcatcacccg tcttgccgcc gttggtagcg tcgccgatag cctggctggc aatgcctgcc
     6241 caattgctgt cctgacgatc aatggtgatg gaaccagcgc tgaccttgac cgtgccagcg
     6301 ttgccaaagg agaaagtagt ggaagatatt ccgccaccat tgacgatcaa aagtgcaccg
     6361 ctggccgtga tgtcgatact gccagcatca cccgttttgc cggcattgtc agcttcgccg
     6421 agcgcctgac tggcaatgcc agtcgtgttg ccctgcccat caatggcgat agagccagcg
     6481 ctgactttga ccgtgccagc catgccggag gaggatgtac tggaggatat tgtgccgcca
     6541 ttggcgatgg aaagcgcacc gctggcagtg atgctgatac tgccagcatc gcccgctttt
     6601 ccgccgttgt cagtgttgcc gatcgaattg ctttcaatgc ttgcca
//
LOCUS       DAOSLL010000205         6635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10499_length_6635_cov_4.042857, whole genome shotgun sequence.
ACCESSION   DAOSLL010000205 DAOSLL010000000
VERSION     DAOSLL010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6635
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10499_length_6635_cov_4.042857"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="PLB25_10180"
     CDS             complement(<1..651)
                     /locus_tag="PLB25_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOE41979.1"
                     /translation="MLLTQEQEMVRDAVRAFALAELWPNAAKWDKEHHFPKDAHQGLA
                     ALGAYGICVPEDQGGAGLDYLTLALVLEEVAAGDGGTSTAISVTNCPVNAILMKYGNA
                     AQKRQWLEPLAQGQMLGAFCLTEPHVGSDASSLRTTAVKDGDSYVINGIKQFITSGKH
                     GDVAIVIAVTDKGAGKRGMSAFLVPTDAPGYVVTRLEDKLGQHSSDTAQISFDNCRV"
     gene            complement(737..1918)
                     /locus_tag="PLB25_10185"
     CDS             complement(737..1918)
                     /locus_tag="PLB25_10185"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HOE41980.1"
                     /translation="MTEAIVIVSAARTPMGSFQGDFSSLAAHDLGGAAIRAAVERAAV
                     APALVDEVLFGNTLMAGQGQAPARQAAFKGGLPISAGAVTLSKICGSGMRAAMFAHDM
                     LKAGSADVLIAGGMESMTNAPYLLQKARAGYRIGHDRMFDHMMLDGLEDAYEPGRSMG
                     TFGEDCAAKFGFTRQQQDQFATTSATRALATIASGGFAAEITPVTIKGRGGDKLISVD
                     EGPGRVKLDKFSSLKPAFKKDGTITAASSSSINDGAAAMVLMRESTAAGLGCKPLARL
                     VAHAVHAQEPNWFATAPVGSVRKALARAGWSVGDVDLWEVNEAFAVVAMALMHDLKLS
                     HDIVNVNGGACALGHPLGASGARIMVTLMYALQARGLKKGVATLCIGGGEATAVALEM
                     L"
     gene            complement(1993..3843)
                     /gene="aceK"
                     /locus_tag="PLB25_10190"
     CDS             complement(1993..3843)
                     /gene="aceK"
                     /locus_tag="PLB25_10190"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HOE41981.1"
                     /translation="MFPTRLDSSVAYDIAKAMMDGFNRHYQLFRTASGRAKQRFEAAD
                     WVGQQRAQRNRIEYYDLRVRECLARLEQDFKAGEQPMDIWQQVKLHYIGLLVDHHQPD
                     LAESFFNSVTTRILHSSYFHNDFIFIRPAVSTEYIENGEPEAYPTYRAYYPTSPQMQD
                     VLLQIVQDFDLQCEFEDLTRDVSLVMGVIHSLVGKFKLQTNCQFQVLTSLFFRNKGAY
                     IVGNMINGFNEYPFALPLLYTPGDKQLVIDAMLCGADELHMLFGFAHAYFLVDMEIPS
                     AYVQFLHRAMPLKPRNEIYNALGLAKQGKTLFYRDFLHHLRHSRDKFRISPGIKGMVM
                     LVFDLPSYPYVFKLIKDFYPPPKETSREQIKGKYLLVKQHDRVGRMADTLEYSLVAFP
                     RHRFTDELIAEIKKFAPSQIEISERDRDGQTEVILQHVYIERRMIPLNIYLQEAFDAG
                     GADANNTSPGALYAREQLERGVIEYGNALKDLIAANIFPGDMLWKNFGVTRHGKVVFY
                     DYDEIEYITDCIFRKVPTPRCEEDEMSGEVWYTVGPKDVFPETFGPFLLGNPMVREMF
                     MKHHANLLDPAYWQKNKDSILAGHLFDVFPYEQGKRFHQQRISSGPAAAT"
     gene            complement(3916..4572)
                     /gene="can"
                     /locus_tag="PLB25_10195"
     CDS             complement(3916..4572)
                     /gene="can"
                     /locus_tag="PLB25_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HOE41982.1"
                     /translation="MTTSVQDLIDNNRAWAGRIEQQRPGFFSHLAHQQSPKYLWIGCA
                     DSRVPANEITGLEPGEIFVHRNIANVVVHSDLNALSVIQYAVDHLKVEHIMVVGHYGC
                     GGVLAALRGTRVGLADNWLRHVHDVKLRHRHRLDHLTVAAQEDVLCEMNVIEQVGNVA
                     MSNVMQDAWSRGQQVTVHGWCYGLKDGLVKDLGVSMQGSGEVVNVFRHAFKRYPRGTI
                     "
     gene            4904..6454
                     /locus_tag="PLB25_10200"
     CDS             4904..6454
                     /locus_tag="PLB25_10200"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOE41983.1"
                     /translation="MSTFQYYRSNFGKDALASVVVFLVALPLCMGISIASGVPPTAGL
                     ITGIIGGIVVGWLAGSPLQVSGPAAGLTVLVWQIVQQYGLAMMGVIVLLAGLLQLAAG
                     LLKLGQWFRAVSPAVIHGMLAGIGVLIFTSQFHVMLDGRPIGSGIDNLVGLPGAFSHA
                     VADGGAPQAALGIGILTIAVIALWTSFAPKKLKMLPAPLVAIIVAVVTASVLQLKIKY
                     IPDLLANPSDINHIWSVIQFPTLDNLKNVFHSGVLIAAASVAFIASAETLLCASAVDQ
                     MHQGPRTKYDKELAAQGVGNALCGLLGVLPLTGVIVRSSANVDAGGQTRTSAILHGVW
                     LLVFAAVLPFTLAYIPVASLAGLLVFTGYKLAYPKIVPKLLQYGGTEAAIYFSTIVVI
                     VSTDLLKGVLFGLALSIFKLLYIFSHLEIRWEDDKVNNRLNLYLKGSATLIRLPLLAA
                     ELEGVRPGAELHVHFEELDYIDHACLDLITNWEKQHEASGGMLVIEWEELSWKYHQRR
                     AGAQKAAA"
     gene            complement(6491..>6635)
                     /locus_tag="PLB25_10205"
     CDS             complement(6491..>6635)
                     /locus_tag="PLB25_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOE41984.1"
                     /translation="QIFGGNGYINEYPLGRLWRDAKLYEIGAGTSEIRRMLIGRELFA
                     ETC"
BASE COUNT         1303 a         1968 c         1940 g         1424 t
ORIGIN      
        1 cacgcggcaa ttgtcaaagc tgatttgcgc ggtgtcgctg ctgtgctggc cgagcttgtc
       61 ttccaggcgc gtcaccacat agccgggcgc atcggttggc accagaaagg cgctcatacc
      121 gcgcttgccc gcgcctttgt cggtgacggc aatcacgatg gccacgtcgc catgcttgcc
      181 gctggtgatg aactgcttga tgccgttgat gacgtaactg tccccgtctt tgaccgccgt
      241 ggtgcgcagc gatgaggcat cactgccgac atgcggctcg gtcaggcaaa aagcgcccag
      301 catctggccc tgtgccagcg gctcaagcca ctgccttttc tgcgcggcgt tgccatactt
      361 catcaagata gcgttaaccg ggcagttggt gacagaaatc gccgtgctgg tgccgccatc
      421 accggcagcg acttcttcga gcaccagcgc cagcgtcagg taatccagcc cggcaccgcc
      481 ctggtcttca ggcacgcaaa tgccataagc acccaacgcc gccagaccct gatgcgcatc
      541 tttgggaaag tggtgttcct tgtcccattt ggcggcattt ggccaaagtt cggccaaggc
      601 aaaagcgcga actgcgtcgc gcaccatttc ctgttcttgg gtcagcagca tgatcagctt
      661 cctggttgat taactcacag attcatgtca aattggcctc tagcccatat cagtaaagcg
      721 ccaaaagcta ctattactat agcatttcaa gcgccactgc ggttgcctcg ccgccaccga
      781 tgcacagcgt ggcaacccct ttcttcaggc cgcgcgcttg cagcgcatac atcagcgtga
      841 ccatgatgcg ggcaccgctg gcacccagcg ggtgacccag cgcgcaggct ccgccattga
      901 cattgacgat gtcgtggctt agcttgaggt catgcatcag ggccattgcc accacggcaa
      961 aggcttcatt gacctcccac agatcgacat cgccgacgct ccagccagcc cgggccagcg
     1021 ccttgcgcac cgagccgacc ggtgccgtgg caaaccagtt gggttcctgc gcatggaccg
     1081 cgtgcgcaac cagccgcgcc agtggcttgc agcccaggcc cgcagcggtg gattcacgca
     1141 tcagcaccat ggctgccgca ccgtcgttga tgctggaact gctggcagca gtgatggtgc
     1201 cgtctttttt gaaagccggt ttgagcgaac tgaatttgtc gagcttgacc cggcctggcc
     1261 cctcgtcgac gctgatcagc ttgtcgccgc cgcgcccttt gatggtgacc ggggtaattt
     1321 cggcggcgaa tccgcccgat gcaatggtgg ctagcgcccg cgtggcgctg gtggtggcaa
     1381 actggtcttg ttgttgccgt gtaaatccga atttggcggc gcaatcctcg ccaaaggtgc
     1441 ccatggatcg gcccggctca taagcgtctt ccaggccatc gagcatcatg tggtcaaaca
     1501 tgcggtcatg gccgatacgg tagccagcgc gtgccttttg cagcaggtag ggcgcattgg
     1561 tcatgctttc catgccaccg gcgatcagca cgtcggcact gccggccttg agcatgtcat
     1621 gcgcaaacat cgccgcacgc atgcccgagc cacagatttt ggacagggtc acggcaccgg
     1681 cgctgatcgg cagaccaccc ttgaacgcgg cctggcgcgc cggtgcctgg ccctgaccgg
     1741 ccatcagggt attgccaaac agcacttcgt ccaccagcgc cggtgccaca gcggcacgct
     1801 ccaccgccgc ccggatggca gcgccaccca ggtcatgcgc ggccaggctg gaaaaatcac
     1861 cctgaaagct ccccatcggg gtgcgggcgg cactgacgat cacaatggct tcggtcatat
     1921 cattccttat caataaaaag tggttccagc ccttatggta caagcgctgg cagctatgtt
     1981 aaaagtagtg aatcaggtgg ctgccgctgg cccggaactg attcgctgct ggtgaaagcg
     2041 cttgccttgc tcatacggaa acacgtcaaa caggtggccc gccagaatgc tgtccttgtt
     2101 tttttgccaa taggccgggt caagcaggtt ggcgtggtgt ttcatgaaca tctcgcgcac
     2161 catcggattg cccagcaaaa acggcccaaa ggtctcggga aacacatcct tggggccaac
     2221 cgtataccag acctcgccgg acatctcgtc ttcttcacag cgcggtgttg gcaccttgcg
     2281 aaagatgcaa tcggtgatgt actcgatctc gtcgtagtca taaaacacca ccttgccgtg
     2341 gcgcgtgaca ccaaagtttt tccacagcat gtcgcccgga aagatgttgg cggcgatcag
     2401 gtctttcagg gcattgccgt attcgatcac gccgcgctca agctgttcac gcgcgtagag
     2461 tgcccccggg ctggtgttgt tggcatcagc gccaccggca tcaaaggctt cttgcaggta
     2521 gatgttgagc gggatcatgc gccgttcaat gtacacgtgc tgcaggatca cttcggtctg
     2581 gccatcccgg tcgcgctcgc tgatttcaat ctggctgggg gcaaatttct tgatttcggc
     2641 aatcagctcg tcggtaaatc ggtgacgcgg aaacgccacc aggctgtatt ccagcgtatc
     2701 ggccatgcgc ccaacccggt cgtgctgctt gaccagcagg tacttgccct tgatctgttc
     2761 gcggctggtt tctttgggtg gcgggtaaaa gtccttgatg agcttgaaca cataagggta
     2821 gcttggcagg tcaaacacca gcatcaccat gcccttgatg ccgggcgaaa tgcggaactt
     2881 gtcgcgcgaa tggcgcaggt ggtgcaagaa gtcgcggtaa aacagcgtct tgccctgctt
     2941 ggccagtcca agtgcgttgt agatttcatt gcgcggcttg agcggcatgg cgcggtgcaa
     3001 gaactgcaca taggcgctgg ggatttccat gtcgaccaaa aaataggcgt gggcaaagcc
     3061 aaacagcatg tgcagttcgt cggcaccaca cagcatggca tcaatcacca gttgcttgtc
     3121 gcccggcgtg tacagcagtg gcagggcaaa cgggtattca ttgaagccgt tgatcatgtt
     3181 gcccacaatg taggcaccct tgttgcgaaa aaacaggctg gtcaacacct ggaactggca
     3241 attggtttgc agcttgaact tgcccaccag gctatggata acccccatca ccaagctgac
     3301 atcgcgtgtc aggtcttcaa attcgcattg caggtcaaag tcctggacga tctgcagcag
     3361 cacatcctgc atctgcggac tggtcgggta ataggcacga taggtcgggt aggcttctgg
     3421 ctcgccgttt tcgatgtact cggtgctgac cgccgggcga atgaaaatga agtcgttatg
     3481 gaaatagctg ctgtgcagaa tccgggtggt gaccgagttg aaaaatgact cggccaggtc
     3541 aggctgatga tggtccacca gcaggccgat gtaatgcagc ttgacctgtt gccagatgtc
     3601 catcggctgc tcgccggcct tgaaatcctg ctccaggcgg gccaggcact cacgcacccg
     3661 caggtcgtag tactcgatgc ggttgcgttg ggcgcgctgc tggccgaccc agtctgccgc
     3721 ctcaaagcgc tgcttggcgc gccccgatgc ggttctgaac agttggtaat gccggttaaa
     3781 gccgtccatc atggctttgg caatgtcgta agcgacgctg gagtcaaggc gggtaggaaa
     3841 catgtgcttg ctttctgctg ggttgcgggc tgggtcagcc aggcactcgc ctggcataga
     3901 tcgtcccgat caacctcata ttgtgccccg tgggtaacgc ttgaaggcgt ggcgaaaaac
     3961 gttgaccacc tcgccgctgc cttgcatgct gaccccgagg tctttcacca aaccgtcttt
     4021 caggccataa caccagccat gcaccgtcac ctgctgaccc cggctccagg catcttgcat
     4081 cacattgctc atggcgacat tgccgacctg ctcgatcaca ttcatctcgc acagcacatc
     4141 ctcctgggcc gccaccgtca ggtgatcaag ccggtggcga tggcgcagct tgacatcatg
     4201 cacatggcgc agccagttgt cggccaggcc aacccgggtg ccacgcaggg ccgccagcac
     4261 gccgccgcag ccgtaatgcc cgaccaccat gatgtgctcg actttgaggt gatcgaccgc
     4321 atactgaatg accgacagcg cgttcaggtc ggagtgcacc accacgttgg caatgttgcg
     4381 gtgcacaaat atctcgccgg gctccagccc ggtgatctcg ttggccggca cacggctgtc
     4441 tgcgcagccg atccacaggt acttgggtga ctgctggtgc gccaggtggc tgaaaaatcc
     4501 cgggcgctgc tgctcaatgc gcccggccca agctcggtta ttgtcaatca ggtcttgaac
     4561 ggaagtggtc atgcaaaatg cctaaaaaat ccaactgtag ccgaaatgaa accaagcaaa
     4621 cgcgctggct gcggcgctgc caggctcggc cagcaaacgc tgcatgtggg tcgcgccggg
     4681 tcaaaatgca gcttcttgat ccgttaaact gcagcggctc atttcatttc taggccattc
     4741 gtgtctgggc agcctgccgc agacattgca aacgcaaggc aaggcgagat cgcaacgaca
     4801 tggatgattt agaaatggaa tcagacactt gattgccgag acatcgggcc agcaagtgcc
     4861 aaaatattcc tcatcagcga aaggtcgaaa gatggcccac accatgtcaa cattccagta
     4921 ttaccgcagc aatttcggca aagatgcgtt ggcctcggtg gtcgtttttt tggtcgccct
     4981 gccgctgtgc atggggattt cgattgcctc cggggtgccg cccacggccg ggctgatcac
     5041 cggcatcatt ggcggcattg tggtcggctg gctggccggt tcgccgctgc aggtgagcgg
     5101 cccggccgct ggcctgacgg tgctggtatg gcaaatcgtg cagcagtatg gcttggcgat
     5161 gatgggcgtg attgtgctgc tggccgggct gctgcagctc gcggccggcc tgctcaagct
     5221 gggccagtgg tttcgggcgg tgtcgccggc agtgattcac ggcatgctgg cgggcattgg
     5281 cgtgctgatt tttacctcgc agtttcatgt gatgcttgac ggacggccga ttggctcggg
     5341 cattgacaac ctggtcggcc tgcccggtgc cttttcgcat gcagtggcag atggcggtgc
     5401 gccacaggcc gctttgggca ttggcattct gacgatcgcc gttattgcgc tatggaccag
     5461 ttttgcaccc aaaaaactca agatgctgcc ggccccgctg gtggccatca tcgtcgccgt
     5521 ggtcactgcc tctgtgctgc aactcaaaat caagtacatc ccggatttgc tggccaaccc
     5581 aagtgacatc aaccacatct ggtcggtgat ccagtttccg acgctggaca atctgaagaa
     5641 tgtttttcac agcggggtgc tgatcgctgc tgcttcagtg gcctttattg ccagcgccga
     5701 aaccctgctg tgcgccagcg cggttgatca gatgcaccag gggccgcgca ccaaatacga
     5761 caaggagctc gccgcccagg gtgtcggcaa tgcgttgtgc gggctgctgg gcgtgttgcc
     5821 gctgaccggg gtgatcgtgc gctccagcgc caatgtcgat gcgggcggcc aaacccgcac
     5881 ttctgccatt ttgcatgggg tatggctgct ggtgtttgcg gcggtgctgc cgttcacact
     5941 ggcctatatt ccggtggcca gcctggccgg cctgctggtg tttaccggct acaagctggc
     6001 ctaccccaaa atcgtgccca aactgctgca gtatggcggc accgaggcgg ccatttactt
     6061 ttcaaccatc gtcgtcattg tctcgaccga cttgctcaag ggtgtgctgt ttggcctggc
     6121 gctgtctatt ttcaagttgc tgtacatctt ctcgcacctg gaaatccgct gggaagacga
     6181 caaggtcaac aatcgcctta acctgtatct gaaaggctcg gcgacgctga ttcgcctgcc
     6241 tttgctggca gccgaactcg aaggggtgcg ccctggggca gaactgcacg tccattttga
     6301 agagctcgac tacattgacc atgcctgcct ggatctgatc accaattggg aaaaacagca
     6361 tgaagcatcg ggcggcatgc tggtcatcga gtgggaagag ctgtcttgga aataccatca
     6421 gcgccgggcc ggcgcgcaaa aagcagccgc ctaagtcagc cccaaaagca cccgctgcgc
     6481 gggtgccctg tcagcaggtt tcagcaaaca gttcgcggcc aatcagcatg cggcgaattt
     6541 ccgatgtgcc agctccaatt tcgtacagct tggcatcgcg ccatagtcgg cccagcgggt
     6601 actcgttgat gtagccattg ccgccaaaaa tctgc
//
LOCUS       DAOSLL010000206         6626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10513_length_6626_cov_5.081266, whole genome shotgun sequence.
ACCESSION   DAOSLL010000206 DAOSLL010000000
VERSION     DAOSLL010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6626
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10513_length_6626_cov_5.081266"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..177)
                     /locus_tag="PLB25_10210"
     CDS             complement(49..177)
                     /locus_tag="PLB25_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41985.1"
                     /translation="MGKNDNSPIKVAVVEDHPEFRDALCQTLVQSPCFELLEPCKE"
     gene            complement(213..347)
                     /locus_tag="PLB25_10215"
     CDS             complement(213..347)
                     /locus_tag="PLB25_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41986.1"
                     /translation="MSVLNWVKAVVEHQLTFLLVGAAGSMQAATPTVAAINTSYGVFL
                     "
     gene            complement(1047..2141)
                     /gene="modC"
                     /locus_tag="PLB25_10220"
     CDS             complement(1047..2141)
                     /gene="modC"
                     /locus_tag="PLB25_10220"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HOE41987.1"
                     /translation="MSGIQARFRLDWSGFVLDVDLDLPARGVTALFGHSGSGKTTLLR
                     CIAGLERPAIGRLTVDGAVWQDDEPKGQRGSGHWLPTHQRPLGYVFQEASLFPHLTVM
                     GNLQYGLKRSPGAARVSLDQAIEMLGIGHLLDRKPAHLSGGERQRVSMARALAVSPRL
                     LLMDEPLAALDLKRKQEILPYLERLHRELDIPVLYVSHSPDEVARLADHIVVMAEGRA
                     VAVGPLTDTLARLDLPIQLGEDAGVVLQGSVAERDERWHLTRVAFDGGSVWVRDGGQR
                     QAQAVRVRILARDVSIALQPVSASSIQNCLSAVVDEIADDLHPALSLVRLKVGNSPIT
                     ARLTRRSAAALALAPGQPVWAQIKAVALIG"
     gene            complement(2143..2823)
                     /gene="modB"
                     /locus_tag="PLB25_10225"
     CDS             complement(2143..2823)
                     /gene="modB"
                     /locus_tag="PLB25_10225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HOE41988.1"
                     /translation="MFLNDSDLQAIWLTVRLAGIVTLILLLIGTPVAWWLARSKAWWK
                     GPVGAVVALPLVLPPSVLGFYLLLAMGPNGLVGQFTQALGLGMLPFTFWGLVLASVFY
                     SLPFMVQPLQTAFEAIGERPLEVAATLRASPLDAFFTVAVPLALPGFLTASILTFAHT
                     VGEFGVVLMIGGNIAGVTRVASVQIYDHVEAMEYLQAHRLSAVMLIFSFLVLLALYAW
                     RPQARKAL"
     gene            complement(3152..3718)
                     /locus_tag="PLB25_10230"
     CDS             complement(3152..3718)
                     /locus_tag="PLB25_10230"
                     /inference="COORDINATES: protein motif:HMM:NF016762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifQ"
                     /protein_id="HOE41989.1"
                     /translation="MNPSHDCRDLLLTELLGRPVVARAAADPLRPVLASLLAGRSLNQ
                     GLLPATLGLPESALQALWHDYFPGARLNLQSGPVADMAELDDIRQLLLAYRAGSHDSE
                     AWLAHSVAYGCGGRNHLWQDLGLADRGELSCLMTTAFPALAALNTGDMKWKKFIYRHY
                     CSQEGIYVCPAPSCGECADHAKCFAPEV"
     gene            complement(3715..4041)
                     /locus_tag="PLB25_10235"
     CDS             complement(3715..4041)
                     /locus_tag="PLB25_10235"
                     /inference="COORDINATES: protein motif:HMM:NF014769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41990.1"
                     /translation="MHALNDSQLLALLEDDVPCADLTTDTVGIGEQSAQIEFRARFAM
                     TVCASEEAARMFESAKADRAAGGIRSDNAGAYGAGGAAEADFLVTSAAYMAVPSDVQV
                     NFGKSG"
     gene            complement(4043..4429)
                     /locus_tag="PLB25_10240"
     CDS             complement(4043..4429)
                     /locus_tag="PLB25_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41991.1"
                     /translation="MKIAVAAKDNWAQVAGHAGQARHWLVFDCQPGAELPEPVRVELA
                     REQLPHHFKDEGPHPLHGVEILVAGSAGDGYVRHMDKWGAQVLLTGETEPLVALNKIL
                     AGEALLDTRFDITTSLCKLRDLFSRH"
     gene            complement(4547..5287)
                     /gene="modA"
                     /locus_tag="PLB25_10245"
     CDS             complement(4547..5287)
                     /gene="modA"
                     /locus_tag="PLB25_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE41992.1"
                     /translation="MIALLLSPLIAASFGVRADEISVAVAANFNAPMQKIAAGFEKET
                     GHKLLTSFGATGKFYAQIKNGAPFDVLLAADDETPARLIRENAAVAGSQLNYAIGKLV
                     LWSAKPAVVDGAGEVLKHGGFDHLAIANPKLAPYGAAAVEVMKALGVHDALQPKLVTG
                     ESIAQTYQFTSSGNALLGFVALSQVLKDGKMEGSYWLIPAKLYTPIRQDAVILEKGKG
                     KPAVQALMNYLKADKAKAIITSFGYALP"
     gene            complement(5347..6168)
                     /locus_tag="PLB25_10250"
     CDS             complement(5347..6168)
                     /locus_tag="PLB25_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOE41993.1"
                     /translation="MRAPQAKLTGRLLIDTAMGGFLGDKRIRLLEAIDKTGSISQAAK
                     AVPFSYKAAWDAVDDMNNIAPEPLVNRSTGGRHGGGTELTAFARRLIAFYRALEQESQ
                     RALEKLTANLTQSGVQDVADFRQALRRMSMKTSARNQFAGPVVAIKEGVVDTEVTLQL
                     APGLELVAIVTRESAENLELTLGRDVLAFVKASSILLMAGEAGRISARNRFTGNVTKL
                     HQGPVNAEVTLTLPGGQHVITAVVTDDSVNRLGLAPGQAVTAVFKASSVFLAAID"
BASE COUNT         1359 a         2038 c         1929 g         1300 t
ORIGIN      
        1 ttttagcggc tcacaattct taaagccccc ggctttgccg ggggatggtt actctttgca
       61 tggctccagc agttcgaaac aaggtgactg gaccagggtc tggcacaagg catcacgaaa
      121 ctcggggtgg tcttcaacca ctgcgacctt gatcggtgag ttatcgtttt tccccatgct
      181 gttcttgtgc tgattgacct gaaatacggc acctacaaaa acacgccgta gctggtgtta
      241 atggctgcca cagtgggagt agctgcctgc atagaacctg ccgcgcccac caatagaaaa
      301 gtaagttgat gttcgactac agcttttacc caattcaaca cgctcatttt tttttctcgt
      361 tattgaagac aagacctttg ctcaagcagg tcaccgcgaa ttgatgctca gatccaaaac
      421 ccggccaggc aaggtgcagc caaaaattgc ctgggcagga agacagatgg ggcaatgcca
      481 aggatcaatt cgcgaacaat ataaccagac tcttcactct tcgatatccc ccatttgggg
      541 gaatattcag agttacttgc gctatttgcg tgattttctg atccaagtca ataaaacgta
      601 accagttgca atgtgtcgtt gctcgaccta accgtcgccg agagctatta tcagcactca
      661 acgtgcttca ttcgtccaat aagtggaagt tgctggtgct ttccttgacg aagttctcgc
      721 gacaaagctg tccgtcacca ctagcagcct gtcggacttg gtctctaaca agtcaagtct
      781 ttgcatgtca tgcgaagaat tcaaacattc catgtcattc gccgcgaggg cgcaactcct
      841 acaaattcaa atcggcgcag actatttgtg taggggtcgc gccctcgcga cgaaggccgt
      901 ccaattccgg cttgtccggc ttcagaagtc gtggcaacag cgacacagcc aaaccttgct
      961 gctgtactga cgcttggtgt ctagccccgc cagcaggggc tcaacgacgc gaatcagcgg
     1021 ttcaagacaa tggcaaaaaa ttccggctag ccaatcagcg ccaccgcctt gatttgcgcc
     1081 cacaccggct ggcccggtgc cagcgccagc gcggcggcag agcggcgcgt caggcgggcg
     1141 gtgatgggcg agttgccaac cttcaggcgc accaacgaca aggcagggtg gaggtcatcg
     1201 gcgatctcat cgaccaccgc gctcaggcag ttctggatgc tgctggcgct gaccggttgc
     1261 aaggcaatgc tgacatcccg ggccagaatg cgcacccgca ccgcctgagc ttgccgctga
     1321 ccaccatcgc gcacccacac gctgccgcca tcaaatgcca ctcgggtcag atgccagcgc
     1381 tcatcacgtt cggccacgct gccttgcagc accacaccgg catcttcgcc caattgaatc
     1441 ggcaggtcca gccgcgccag ggtgtcggtc agcggcccga ccgccaccgc ccggccttct
     1501 gccatgacga cgatgtgatc ggccagccgc gccacttcgt cgggtgaatg gctgacatac
     1561 agcaccggaa tgtccagctc gcggtgcaaa cgttccaggt agggcaggat ttcctgcttg
     1621 cgcttgaggt ccagcgcggc cagaggctcg tccatcagca gcaggcgcgg gcttaccgcc
     1681 agggcgcgcg ccatgctgac acgctggcgt tcaccgcccg acaagtgggc cggtttgcga
     1741 tccagcaagt ggccgatgcc gagcatttcg atggcctgat ccaggctgac gcgcgccgcg
     1801 ccggggctgc gcttgagtcc atattgcaga ttgcccatca cagtgaggtg gggaaacaaa
     1861 ctggcttcct gaaacacata gccaagcggt cgctggtggg tgggtagcca atggccactg
     1921 ccacgctgtc ccttgggctc atcgtcttgc cagaccgcgc catcaaccgt gaggcgaccg
     1981 atggcggggc gctccagccc ggcgatgcag cgcagcaagg tggttttgcc cgagccggaa
     2041 tggccaaaca gcgcggttac cccgcgcgct ggcaaatcca gatcgacatc gagcacaaag
     2101 cctgaccagt cgaggcgaaa gcgcgcctga atgccgctca tctcagagcg ccttgcgagc
     2161 ctggggccgc caggcgtaca gcgccagcag caccaggaac gagaagatca gcatcacggc
     2221 tgacagccga tgcgcttgca ggtattccat cgcctccaca tggtcataaa tttgcaccga
     2281 agccaccctg gtgacgcccg caatgttgcc gccaatcatc agcaccacgc caaattcacc
     2341 caccgtgtgg gcaaaggtca ggatggatgc agtcaaaaac cccggcagcg ccagcggcac
     2401 cgccacggta aagaaggcat ccagtggcga cgcgcgcaag gtggcagcga cttcgagcgg
     2461 gcgctcgcca atcgcctcga aggcggtttg caagggctgc accatgaagg gcaaggagta
     2521 aaacaccgag gccagcacca ggccccagaa ggtgaacggc aacatgccca ggcccagtgc
     2581 ctgggtgaac tgccccacca gcccatttgg ccccatcgcc agcagcaggt aaaaacccag
     2641 caccgagggc ggcaacacca gcggcaaggc caccaccgcg ccaaccggcc ctttccacca
     2701 ggccttgctg cgcgccagcc accaggcgac cggcgtgcca atcagcagca aaatcagcgt
     2761 cacgatgccc gccagacgca ccgtcagcca gatggcttgc agatcactgt cgttcaaaaa
     2821 catgggttga ttctttgcgc ggttgagatt gaccgtttat tttttccgga agccatcgcc
     2881 aagtgcgcag cgccagcgaa tcttctttta gctatgcaaa atatagctgc cagcgcatac
     2941 tgtgattggg ctagaggctc atttgttata taaagtaaag ttgcaacatg ggcggaccag
     3001 cgccccttcc tgcgcaactt gcgcggcttg ttgcgctggt ttttgcagga ggggcgctgg
     3061 tccgcgatgg ctgtgactgg tagcagcaca acggtcaatt tcagccgttc gaggtgtatc
     3121 ttagcggctg gtccggtgcg cattggcggg ctcacacctc aggggcgaaa cacttggcat
     3181 gatcggcaca ttcgccacaa gacggcgccg ggcagacgta aatgccttct tgtgagcagt
     3241 aatggcggta gatgaatttc ttccatttca tgtcgccggt gttcaaggcc gccagtgcag
     3301 gaaaggctgt ggtcatcaag caagagagct cgccccggtc ggccaagcca aggtcttgcc
     3361 aaaggtggtt gcgcccgccg cagccataag ccacgctgtg cgccagccag gcttcagagt
     3421 cgtggctacc ggcacggtag gccagcagca gttgccggat gtcgtcaagc tcggccatgt
     3481 cagcgaccgg gccgctttgc agattcagcc gggcaccggg aaagtagtcg tgccaaagcg
     3541 cttgcaaggc cgactctggc aaacccagcg tggcgggcag caacccttgg ttcaatgagc
     3601 ggcccgccag caggctcgcc agcaccgggc gcagcgggtc ggctgcggcg cgcgcgacga
     3661 ccggtcggcc cagcagttcg gtcagcagca ggtcgcggca gtcatgcgac gggttcatcc
     3721 ggactttcca aaattgactt gcacgtcgct gggcactgcc atgtaagctg ccgatgtcac
     3781 caaaaagtca gcctccgcag ccccgccagc cccatacgcg cccgcgttgt cagaccggat
     3841 gccgcccgca gcgcgatccg ctttggccga ttcaaacatc cgggcggcct cttcactggc
     3901 acaaacggtc atggcaaagc gcgcccgaaa ttcgatctgg gcagactgtt ccccaatgcc
     3961 cacggtgtcg gtggtcaggt cggcgcaggg cacgtcatct tccagcaagg ccagcaattg
     4021 gctgtcgttc aaggcgtgca tgtcaatggc gcgagaacag gtcgcgcagt ttgcacaggc
     4081 ttgtggtgat gtcgaaacgg gtgtccaaca gggcctcgcc cgcgaggatt ttgttcaagg
     4141 caaccagggg ctcggtttcg ccggtcagca gcacctgagc gccccacttg tccatgtggc
     4201 ggacatagcc atccccggcg ctgccagcca ccaaaatctc gacaccatgc agcggatgcg
     4261 ggccttcgtc cttgaagtga tgcggtagtt gttccctggc gagttcaacc cgcactggct
     4321 cgggcagctc agcgcccggc tggcagtcaa acaccagcca gtggcgggcc tggcccgcgt
     4381 ggcctgccac ctgcgcccag ttgtctttgg cggcaacagc aattttcatg atgatcgacc
     4441 catggtatgg atgcccgctg aagtgaatct gccagtcacc aagcgagttt cgtgcccgct
     4501 ggccgggttc gctgtttctg ccggttttct gtcgggcaac cgccggtcaa ggcagcgcgt
     4561 agccaaacga ggtgatgatg gctttggcct tgtcggcttt cagatagttc atcagggcct
     4621 gcactgccgg tttgcctttg cccttttcca gaatgaccgc gtcctggcgt atcggtgtgt
     4681 acagtttggc gggaatcagc cagtaagagc cttccatttt tccgtccttc aagacttgcg
     4741 acagcgccac aaagcccagc agggcgttgc cgctgctggt gaactggtag gtttgcgcga
     4801 tgctctcacc ggtcaccagc ttgggctgca gcgcgtcatg cacgcccagc gccttcatca
     4861 cctcgaccgc agccgcacca taaggcgcga gtttcgggtt ggcaatcgcc agatggtcaa
     4921 agccgccatg cttcaaaact tcgcctgcgc catcaacaac cgctggcttg gctgaccaca
     4981 gcaccagctt gccaatcgca tagttgagct ggctgcctgc cacggcggcg ttttccctga
     5041 tcagccgggc cggggtttcg tcatcggcag ccagcagcac atcaaagggc gcgccgtttt
     5101 tgatctgggc gtaaaacttg ccagtggcac caaaagaggt cagcagcttg tggccggttt
     5161 ccttttcaaa cccggcggcg attttttgca tcggtgcatt gaagttggca gcaaccgcca
     5221 ccgaaatctc gtcggcgcgc acgccaaacg aggcggcaat caaaggtgac agcaacaagg
     5281 caatcaggcg ggatggtttc acgacaactc cttcttaaaa caaatgaaca atcagtctgg
     5341 ctcccatcag tcgatggcgg ccaaaaacac gctggaggcc ttgaacaccg cagtcaccgc
     5401 ttgcccaggc gccaggccaa gccgattcac gctgtcatcg gtgacgacgg cggtgatgac
     5461 gtgctgccca cccggcagcg tcagcgtcac ctcggcattg accggcccct ggtgcagctt
     5521 ggtgacgttt ccggtgaaac ggttgcgcgc cgagatgcgg cctgcttcgc ccgccatcag
     5581 tagaatcgac gaggctttga caaaggccag cacatcccgt cccagcgtca actccaggtt
     5641 ttcggccgac tcgcgggtga cgatggccac cagttcaaga ccgggggcca gttgcagggt
     5701 aacttcggtg tccaccaccc cttccttgat cgctaccacg gggccagcga actggttgcg
     5761 ggcactggtt ttcatcgaca ttctcctcaa ggcttgccga aaatcggcga cgtcctgcac
     5821 accgctttgc gtcagattgg cggtcagctt ttccagtgcg cgctgggatt cctgctcaag
     5881 cgcgcggtaa aaggcaatca gccgccgggc aaaggcggtc agttcggtgc cgccgccgtg
     5941 gcgacccccg gtggagcgat tcaccagcgg ctcgggggcg atgttgttca tgtcatcgac
     6001 cgcgtcccac gccgccttgt acgaaaacgg aaccgctttg gccgcttgcg aaatggaacc
     6061 ggttttgtca atggcttcaa gcagccggat gcgcttgtcg cccagaaagc cgcccatggc
     6121 ggtatcgatc agcagccgac cggtgagttt ggcttggggc gccctcatgt tggcatcacc
     6181 gggtcaagcg gttgggtctg cctcgcgccc aagccagccg ggcgttgggc tggcaaaggc
     6241 ccaacagcct tttgcaccat gcaaaccttg ctgcttgcgt taaccgtctg tgtccttggc
     6301 accatgccca ttgaattctc caaaagcgct tgcgtgtgtg cctgggcgac acgccatgtc
     6361 tgaaaaaccc cgctgctttg tcttgttgac gacagggcgg gtctggttgg ctgcgcgctg
     6421 ttgtcggcat cccgacaaac ggcagcatcg ttataacctg cagcaaatag cgtgccgctt
     6481 tttgggtgcg gagccagcaa caaaaaccca tgcgggcagg ctcgatgggc gggattcttg
     6541 aaaagggcag cgtcgcgatg cgaaccatct gccgctggca agttctttca aacgctatat
     6601 aaaagttagc tgcttgcgct tattag
//
LOCUS       DAOSLL010000207         6615 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10542_length_6615_cov_4.446799, whole genome shotgun sequence.
ACCESSION   DAOSLL010000207 DAOSLL010000000
VERSION     DAOSLL010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6615
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10542_length_6615_cov_4.446799"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            349..489
                     /locus_tag="PLB25_10255"
     CDS             349..489
                     /locus_tag="PLB25_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41994.1"
                     /translation="MVAGASLAEEIEALQAPMRSLRSDLVLVQLHAMMARQIVPHDQH
                     TP"
     gene            486..1514
                     /locus_tag="PLB25_10260"
     CDS             486..1514
                     /locus_tag="PLB25_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOE41995.1"
                     /translation="MKTPLHRILAIDDTPANLLALDGLLSNEFDLQFATSGPTGIALA
                     LKCPPDLILLDVMMPEIDGFETFKLLAAQPMLKAIPVVFVTALNDFDAEVAGLSLGAA
                     DFITKPINATIARQRIRNLIERKREQDALQQALQEKVALLKEVHHRVKNNLQVIASLL
                     RMEGKGSEHSHTQAVLNDMQGRIRAMALLHESIYQNGTFAWVDLASYLKKVATQVFVS
                     LAVSPSLIRLQLQLDELQVSLDQAMPCGMLVSELISNAFKHGFPDGRSGEVCIELRPL
                     EDGAMWQLRVSDNGIGLPDNFESAVQHSLGLQLASSLASQMGGRLDVGPGAAFTVQFK
                     VEPTTLPT"
     gene            1556..1960
                     /locus_tag="PLB25_10265"
     CDS             1556..1960
                     /locus_tag="PLB25_10265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE41996.1"
                     /translation="MTHQKPGDFGQARILIVEDESIIAMDIAMQLRELGHQVVGQASS
                     GEQAINLAQRLRPDLVLMDVQLKGVMDGIDAAQTIRSRFNLPCLFLSAFANDSDSLTR
                     AALAAPVGYLSKPFFDHELRSVMEIAIRQLDQ"
     gene            2441..3091
                     /locus_tag="PLB25_10270"
     CDS             2441..3091
                     /locus_tag="PLB25_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41997.1"
                     /translation="MKFFLAVLLSFLALVTYSQATDFKFSTADNEDDAEAQRQQAKVA
                     ALLLTPCRNKIKNQKIMVLIGEEHNGRVSTNQSLFSPHFDAINSRLQTMGLRTYTQAQ
                     IRAQVAQAEIDAYFKNDPDAAISASRRLAAQYILRGYISTQASRNAIIGVNQVNVRMS
                     FTLTGSSGRTISQASAEEVSYAGQDTSGMALTLINEKADEVVATLYNDYCNQSGVR"
     gene            3137..3952
                     /locus_tag="PLB25_10275"
     CDS             3137..3952
                     /locus_tag="PLB25_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE41998.1"
                     /translation="MQTGIPNHSMSASCVVIGLFLTIGSALAQPSFGNPSPTAGTATS
                     DKANAAADAAAYKEVEYKNRNLVGPALVVIPGEIKSNNATFVQKFGPNNIADFAELEL
                     SNANFKVLERQNLGNLLQEISLAYNLGDPVAARKTMQVGKLKTTKWIVKFDILKAEQI
                     AESSKGFDGRAIGGLMSMFGGRAGAAAGSVTGSVQNSDSTGVWLVGMRYKIMDANTTE
                     QVAQGYKDLKMEVGATQTSVLGVSESAKGGVGLDTMVQRLVQTLVWDIDAKHK"
     gene            3982..6420
                     /locus_tag="PLB25_10280"
     CDS             3982..6420
                     /locus_tag="PLB25_10280"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOE41999.1"
                     /translation="MTTISKIGKYLIRRELGQGAMGIVYEGFDPVIERTVAIKTILPS
                     KLTAADSTETLARFKREAQSAGRLNHPGIVAIYEYGEVVPERAGAPIDPDATLVDASD
                     VSPDATAIGSASGQRIAFIAMEFVKGRELKDFFEANERFKPADVARLMGEILAAMGHA
                     HAHGVVHRDIKPANLIVLDDGRVKIADFGIARIERSELTQMGTIMGTPSYMSPEQFMG
                     QTVDGRSDLFSCGVVLYQLLTGEKPFTGNTTTIMYKVLREEPIAPSLLNLALPPAFDE
                     VLRKAIAKSPEQRFQTAAEFNAAVQAALSRSATVKPAPVAPAIAAAAASPAAPIMAPV
                     AAQPTVPLPVIRERAPGKFLWAGAAALISLAVVGIGTFLWTATGPGNSGKAGVAATTS
                     AVVAPSATPALPTAVVPVAPSAANDEATRGTLVVSALGLVDPADPRFHGDAVAARAEA
                     QADARRQLVEKVLVLYVEKKSLESNYPLLEQKLLSQAGSFIKTTLREDAPIAGKDGLL
                     QAQSRAVINVRDIQKSLNQLSKEERINFIRNNGDPRVAIAMTVTNVESATAMPSARSQ
                     LAENVLKERIKSFGFRVWANDGEVGTTAQSADFLIQGDAKIKHLSMKLPASGLTITKS
                     VLTSWTVKASDKLTGEEIYLNTLVPKGQSWASEDQALVDIGRLVGDEFSRDFFLQHFS
                     AGVVSTRLNIAGLPDAATAQLLLRELRGIRQVLDVQLIDAKGSYSLELAQGNAGEIVQ
                     EAVMRPLNAKLGQNCFAFAGANSTEVRLTYSNTCQTAAIAGRFETAPPAGVLTAPAQR
                     SAPLLKNAAVKAIT"
     gene            complement(6424..>6615)
                     /locus_tag="PLB25_10285"
     CDS             complement(6424..>6615)
                     /locus_tag="PLB25_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOE42000.1"
                     /translation="LRQEKFFLVDQSTNGTFVLFDGEPEITLRREQVMLQGTGCISFG
                     RSLQDASEDIVSFCVEDAL"
BASE COUNT         1535 a         1839 c         1824 g         1417 t
ORIGIN      
        1 gcccaggaaa ccgccgtagg caaacgcgac cagggccatc accgcaaaga tgcttttgaa
       61 gaagaccacc aacgttgcct ggctttgggc ataggtgatt tttaaccatc ccgatcaccc
      121 ttcataggct gtgaaactgg cacttgttaa gtggttaccg gttgcctctc atgccattgc
      181 tcaatcatcc atttcgctgt tacatcgtct tgccctgcgt ttccaatgtc tgcggcaggg
      241 ttgcgctgaa ggtgccagac ttgaacgcat ttaccgactt gtttgccaaa cttgcgccac
      301 tgcccgagca aaacaggttc aatgcgatgc ttcaggaaac tgcagcactt ggtggctggt
      361 gcatcgctgg ccgaagaaat tgaggcgctg caggctccga tgcgtagtct gcgctctgat
      421 ctggtactgg tacagttgca cgcaatgatg gccaggcaga tcgtgcccca tgaccaacac
      481 acaccatgaa aaccccactt catcgcattc tcgccattga cgacacccca gccaacctgc
      541 tggcactcga tgggttgctt tccaatgaat tcgacctgca atttgccact tctgggccaa
      601 ccggcattgc gttagccctc aaatgcccgc ctgatctgat cttgctagat gtcatgatgc
      661 ctgaaattga tggatttgaa accttcaagc ttctggcggc acagcccatg ctcaaagcca
      721 taccagtggt atttgtcacg gccttaaacg actttgatgc cgaagttgcc ggcttgtcct
      781 tgggcgcggc tgacttcata accaagccca tcaatgccac catcgcgcgc cagcgcattc
      841 gaaatttgat tgagcgcaaa cgtgaacagg atgctctgca gcaagccctg caggaaaagg
      901 tcgccctgct caaggaagtc catcaccgcg tcaagaacaa cctgcaggtc atcgcaagtc
      961 tgctgcgcat ggagggcaag ggtagtgagc acagtcatac tcaagccgtt ctcaacgaca
     1021 tgcaggggcg cattcgcgcc atggcgctgt tgcatgagtc aatctaccaa aacggcactt
     1081 ttgcctgggt ggatcttgcc agctatctga aaaaggttgc cacacaggtt tttgtttctc
     1141 tggccgtgtc accaagtctc attcggctgc aactgcaatt ggacgagcta caggtgagcc
     1201 tggatcaggc catgccgtgt ggcatgttgg tcagtgaact tatcagcaac gccttcaagc
     1261 atggctttcc tgacgggcgg tcaggcgagg tttgcattga gttgcggcct ttggaggacg
     1321 gtgcaatgtg gcaactgcgc gtcagtgaca acggcatcgg gctacccgat aattttgagt
     1381 ctgcggtgca gcattcattg gggctgcaac tcgcctcaag tttggctagc cagatgggcg
     1441 ggcgcctgga tgtggggcct ggcgcagcgt tcactgtgca gttcaaagta gagccgacga
     1501 cgttgcccac atgatgctgt tggcagtgct cgggcgcgca acatgagatg aagtcatgac
     1561 acaccagaaa ccaggcgatt ttggtcaagc cagaatactc attgtggaag acgagtccat
     1621 cattgcgatg gacattgcga tgcagttgcg ggaacttggc caccaggttg ttgggcaggc
     1681 aagcagcggc gaacaggcga tcaatctggc acaacgactg cgcccggacc tggtgctgat
     1741 ggatgttcag ctcaagggtg tcatggatgg cattgatgcg gcgcagacga ttcgctcccg
     1801 attcaacttg ccgtgcctgt ttctaagtgc ttttgcaaat gacagcgaca gcctcacccg
     1861 tgccgcattg gcagccccgg tcggctacct gtccaagccg ttttttgacc atgaactgcg
     1921 atccgtgatg gagattgcga tcaggcaatt ggaccaataa ccgcgccagc actctcgcgc
     1981 caactgcgca cttgaaatca gttggcctga tcaatggcca gcgaagtggc ttggtaccgg
     2041 tgtcaaatct gccacgcacg ccatcaaatc ccgagaccgc caagcgcata ccagccttga
     2101 agctggtcag gaatgccaag gtctcagtct tggctatcct gtcaaattta acgggttcaa
     2161 ttgaccacgg ttcgatgctt ccccaaggca gagtcaagca gtttaccgca catggatggg
     2221 ctcaatgctg gtcaaaattg ccgaagctca accgctcaaa cttcagttga gcctcaattt
     2281 cccatgccac gcgcggctcc tggggcatgg agaattgaaa attgcccatg ggtaaactgc
     2341 attgcctata attttcggca cggatcaagc tgcctcaagc atgcctgacg acacgccagc
     2401 caagttggtc tttaccaccc cttttgatcg gtcacgaaca atgaagtttt tcctggcagt
     2461 attgctgagt tttctggcat tggtgacgta tagccaggcg acggatttca agttttcaac
     2521 ggcagacaat gaagacgacg cagaggcgca aaggcagcag gcaaaggttg cggccctgtt
     2581 gctgacacct tgtcgcaaca agatcaagaa ccaaaaaatc atggtcttga ttggtgaaga
     2641 acacaacggt cgggtgtcaa ccaatcagtc actgttcagc ccgcatttcg atgccatcaa
     2701 ctcgcggctg caaaccatgg gcctgagaac ttacacgcag gcgcaaatca gggcacaagt
     2761 ggcgcaggcc gagattgacg cttatttcaa gaacgatcct gatgcagcca tcagcgcatc
     2821 cagaagactg gctgcccagt acatcttgcg tggctacatc agcacgcagg ccagccgcaa
     2881 tgcgatcatc ggagtcaatc aggtcaatgt gcgcatgagt tttaccttga ccggttcatc
     2941 gggcagaacg atatcccagg ccagcgctga agaagtgagt tacgccgggc aggacacttc
     3001 aggcatggcg ctgaccttga tcaacgaaaa ggccgatgaa gtggtcgcca cgctgtacaa
     3061 cgattactgc aaccagtcag gtgtcaggtg acacgctgga tctgggcaag cttttaacca
     3121 aacgaaagaa aactccatgc agacaggcat ccctaaccat tcaatgagcg catcgtgcgt
     3181 ggtcattggg ctttttttga caatcggcag cgcgttggcc cagccaagct ttggcaatcc
     3241 atcgccgacc gccggtacag caacgtcgga caaggcaaac gctgcagccg atgcggctgc
     3301 atacaaagag gttgagtaca aaaaccggaa tctggttggc ccggcactgg tggttattcc
     3361 aggtgaaatc aagagcaaca atgccacctt cgtgcaaaaa tttggcccca acaacatcgc
     3421 tgattttgcc gagcttgagt tgtccaacgc caatttcaag gtgctcgagc ggcagaatct
     3481 ggggaatttg ctgcaagaga tttcgctcgc ctacaacctc ggtgatccag tcgctgcgcg
     3541 caaaaccatg caggtcggca aactcaagac caccaaatgg atcgtcaagt tcgacatcct
     3601 gaaagccgag cagattgccg aaagcagcaa gggttttgat ggccgcgcaa tcggtggttt
     3661 gatgtcgatg tttggcggac gagccggggc agcggcaggt tcggtgacag gttcagtaca
     3721 gaacagcgat tcgaccggtg tctggctggt tggcatgcgt tacaaaatca tggatgccaa
     3781 taccactgaa caggtggcac agggctacaa agacctcaaa atggaggtcg gtgccaccca
     3841 gaccagtgtg ctgggtgttt ccgaatctgc caaaggcggt gtcggtcttg acaccatggt
     3901 gcaacgcctg gtgcaaaccc tggtctggga tattgatgcc aaacacaaat agccaggcaa
     3961 gtcctctttc aggctgagaa aatgaccact atctccaaaa tcggcaaata cctgatcagg
     4021 cgcgaactcg gtcagggtgc catgggcatc gtttatgaag gctttgatcc ggtcattgag
     4081 cgcacggtcg ccatcaaaac aattttgcca agcaaactga ccgccgccga ttcaacagaa
     4141 acactggcgc gcttcaaacg tgaagcccag tccgccgggc gcttgaacca tcctggcatt
     4201 gtggccatct atgagtacgg tgaggtcgtg ccggagcgag ccggtgcacc gattgatcca
     4261 gatgcaaccc tggttgatgc ttcagatgtc tccccggacg cgacagccat tggctctgcc
     4321 agcggccaac gcattgcatt cattgcgatg gaatttgtca aaggccgtga actcaaagac
     4381 ttttttgaag ccaacgagcg gttcaaaccc gccgacgtgg cgcgtttgat gggtgaaatt
     4441 ctggcagcaa tgggccatgc gcatgcccat ggcgtggtcc accgcgatat caagcccgcc
     4501 aacctgattg tgcttgatga tggaagagtg aagattgcgg actttggcat cgcccggatt
     4561 gaaaggtccg agctgaccca gatgggaacc atcatgggca ccccgtcata catgtcacca
     4621 gaacagttca tggggcaaac agtggatggc cgcagtgacc tgttctcctg cggggtggtg
     4681 ctgtaccagc tcctgactgg cgaaaaacca ttcacaggca acaccaccac catcatgtac
     4741 aaggtgctgc gcgaggaacc tatcgcgcca tcactgctca atttggcgtt gccgcccgcc
     4801 tttgacgagg ttctgcgaaa agccatagca aaaagccctg agcagcgttt tcagactgct
     4861 gctgaattca atgctgccgt tcaggccgct ttgtcccggt ctgccacggt caagcccgca
     4921 ccggtcgcac ctgctattgc ggctgctgcg gcttcgcctg cagcccccat catggcgcca
     4981 gtggctgccc agcccacagt acctctgcca gttatccgcg aacgagcacc ggggaagttc
     5041 ctctgggcag gagccgctgc gctgatcagc ctggcggtcg tcggcattgg gactttcctg
     5101 tggactgcaa ctggccctgg caactcagga aaggccggag tcgctgccac aaccagcgcc
     5161 gtcgtcgcgc catccgccac cccggcgttg ccgacagcgg tcgtacctgt tgcaccatct
     5221 gctgctaacg atgaggcgac ccgtggcact ttggttgtca gcgcgctcgg tctggttgac
     5281 ccggccgacc cccggtttca tggcgatgct gtcgccgcca gagccgaagc ccaggccgat
     5341 gcccggcgtc aactggtgga aaaagtgctg gtgctttacg tcgagaaaaa gtcactggaa
     5401 tccaattacc cgttgctgga acaaaagctg ctgtcgcagg cgggctcctt catcaagacg
     5461 acgctgcgag aagatgcacc gattgccggc aaggacggtc tgctccaagc ccagtcacgg
     5521 gcggtcatca atgtccggga cattcagaag tcattgaacc agttatccaa ggaagagcgg
     5581 atcaatttca tccgcaacaa tggcgacccc agagtcgcca ttgccatgac ggtaaccaat
     5641 gtagagtctg ccactgcgat gccatcggcc cgatcacagt tggcggaaaa cgtgctgaaa
     5701 gagcgcatta aatcatttgg ctttcgggtt tgggccaatg atggtgaagt cggtacgact
     5761 gcgcaaagcg ctgatttcct gattcagggc gatgccaaga tcaagcatct ctccatgaag
     5821 ttgcccgctt ccggccttac catcaccaaa tcggttctga cctcgtggac cgtcaaggca
     5881 tcggacaagt tgactggcga agaaatctac cttaacacgc tggtacccaa ggggcaaagc
     5941 tgggcctcgg aggatcaggc gcttgtcgac atcggcagac tggtgggcga tgagttttcc
     6001 agggacttct ttctgcagca ctttagcgct ggcgtggtat caacccgcct gaacattgca
     6061 ggcctgcccg atgccgcgac ggcgcagttg ttgctgcgag agttgcgcgg catccgccag
     6121 gtgcttgatg ttcagttgat tgacgcgaag ggcagctact cccttgaatt ggcgcaaggc
     6181 aatgcgggcg aaattgtcca ggaggccgtc atgcgaccac tcaatgccaa gttgggtcaa
     6241 aactgctttg cctttgcggg ggccaacagc accgaggtca ggttaaccta cagcaacacc
     6301 tgccagactg cagccatcgc tggccgattt gagacagcgc caccggccgg tgttctgaca
     6361 gcaccagccc agcgcagtgc accattgctg aaaaatgcgg cggtcaaggc aataacctga
     6421 tggtcagagc gcgtcttcca cgcaaaagct gacgatgtct tcagatgcat cttgaaggct
     6481 gcgtccgaaa gagatgcacc ctgttccctg cagcatgact tgttcccggc gcagcgtgat
     6541 ttcgggttct ccgtcaaaca gcacaaaggt gccgttggtg ctttgatcaa ccaggaagaa
     6601 tttttcctga cgcag
//
LOCUS       DAOSLL010000208         6595 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10586_length_6595_cov_5.110245, whole genome shotgun sequence.
ACCESSION   DAOSLL010000208 DAOSLL010000000
VERSION     DAOSLL010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10586_length_6595_cov_5.110245"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PLB25_10290"
     CDS             complement(<1..267)
                     /locus_tag="PLB25_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42001.1"
                     /translation="MQKNLLSAAPPTNRLQRSESVKMVGFRKAQCHDFCCRAATDRDQ
                     HHEQSALSVTTVQFRLMPAASAQSAPLDVVRNNFPYLHRRIWAHS"
     gene            complement(421..648)
                     /locus_tag="PLB25_10295"
     CDS             complement(421..648)
                     /locus_tag="PLB25_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42002.1"
                     /translation="MRWSGRATSNSTCGLSMTPLYTENADLTNSPADPRRQDKPLCVN
                     GRIGFGNQQKKQARRQPAQPTQGQIGLSRAV"
     gene            complement(648..893)
                     /locus_tag="PLB25_10300"
     CDS             complement(648..893)
                     /locus_tag="PLB25_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42003.1"
                     /translation="MELSFFDRIVALNQPTQQTTAQLDPTHRLLQRHQHGILSPPALV
                     NNEVCADDTGSLVTNLPVPHRAHRIDTVAIALPCGGR"
     gene            1009..3942
                     /locus_tag="PLB25_10305"
     CDS             1009..3942
                     /locus_tag="PLB25_10305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE42004.1"
                     /translation="MNSIEDSMTQRTERLQLLKQRAQAILKLHDTGFGGGESDSLQVV
                     KLLEDLRVYQVELELQNEELREAQQEADLARKRYQNLFTKMPMPALVVDVNGVVDDCN
                     ELANAHLGLNRRNRAVDARLWQRLKGKDRARLHVALRDLLPGQTLVLPQVVIGEANAQ
                     TPVFDVHMMGLSIDYRLDHRVLLMLVDRTAEIQREQDHRFYASLLDSADSIIYAADKD
                     GQLLLANQAFLDLLGRQRDEVKGSKREAFLPLRDAIFHNETDQKVLATGEALTVHEQS
                     YLAAQRGVTDLLTRKFPLRDSTGRIYGVGGISTDITALKDQQRQGLLSETVFMQAQEA
                     IVITDASTRIIRVNPAFVRQTGFSLESTVGHKTSMLKSGRQSQIFYHDMWQSINNTGC
                     WSGEIENRRADGVYYTVWININAVHDQDRKLLYYIGASTDITEHRKAQAEIQRSAELL
                     RGAIDVIDEAFVLFDADDRLVFCNDKYRSLYATSNDLMVPGALFMDIVRVGAERGQFR
                     DAVGRVDEWVAERMAAHRSGNSSTVVQLDNGRVLRAVERRMPDGHTVGFRADITEFVR
                     ATEEAKAANLAKSRFLATMSHEIRTPMNGILGMAQMLLMPSLSAADQLSYVNTILSSG
                     QTLLSLLNDILDLSKIEAGKIRLDSVTFTPDSLLGDICNLFSGAAQAKGLAMDYQWLG
                     PATQCYQSDASRLRQMLSNLVGNAVKFTSLGKVHLQGSEIERQGDSALLEFAVTDSGM
                     GIAADKLGLLFKPFSQTDNSTTREFGGSGLGLSIVNNLAKSMNGDVGVQSELGRGSRF
                     WFQVRVTVMPDGAPLPGLQGQATEPADLPALSGRVLVVEDNLVNCMVIESLLKRLGMA
                     VTVVNDGQQAVDAITGGGPDSEPDLILMDLQMPVMDGYTAVQQIRQWESAQQRPRHLI
                     IALTADAFAEDHLRCLAGGMDDFLTKPIAIDVLKTALVKWLVPVTARHSILPQGTAPN
                     FARPN"
     gene            4302..6353
                     /locus_tag="PLB25_10310"
     CDS             4302..6353
                     /locus_tag="PLB25_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014004910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cache 3/Cache 2 fusion domain-containing
                     protein"
                     /protein_id="HOE42005.1"
                     /translation="MSNYPSNWHNASLGTKLAISNFVLVALVFSVLIAAIAYSVTSVI
                     ESRAKVELEDRTKLLVEMVSSADKELRSRTSVLARGFQSSLAGKLSIDPATVEIGGKP
                     TPTLSLDGRPLNLEFALVDRFTAATGAVATVFAKTGDDFVRITTSLKDDKGERAIGTL
                     LDRAHPGYKASLTGNSFTGLATLFGRQYMTQYDPIKDAQGQVIGLSFVGLDFTDYLVN
                     LKNTIRSLKVGKEGFFYVLDSRPGSSYGNLVIHPSAEGKNLLNVRDADGREFAKDMLE
                     QKNGLMRYAWVNKELGETSARDKVVSFSYLKGWDWVIAGGTYADEYVIEVNQLRNLYV
                     LLGVVLVIITSGVLFLLIRRIVLVPLARVIGDAQSIAHGDLSVQLQTDRHDEIGKLMT
                     AMNSIGSGLSGVVQSVRTGSESVATASAEIAQGNQDLSARTEQQASALEQTTASMEQL
                     SANVKQNADSARQANALAMSATDVAMQGGTVVTQMVDMMKGINAASNKIADIIQVIDG
                     IAFQTNILALNAAVEAARAGDQGRGFAVVASEVRLLAGRSATAAREIKGLIDASVEQV
                     VQGNALAGQAGNTMTEVVSSIKRVTDLMGEISAASAEQASGVDQIGAAVTHMDQATQQ
                     NATLVEEIAAAASSLKLQASELVETVAVFKLSDDTGYAIAAIGNTAGSSAAAQRRISA
                     T"
BASE COUNT         1411 a         1842 c         1976 g         1366 t
ORIGIN      
        1 gctgtgcgcc caaatccgcc ggtgcaaata ggggaagtta ttcctgacca catccaaagg
       61 tgccgactgt gccgaagccg caggcataag gcgaaattga acggttgtca ccgagagagc
      121 cgactgttca tgatgttggt cacgatcagt ggctgccctg cagcagaaat catgacactg
      181 ggctttcctg aatccgacca tcttgactga ttcagaccgc tgcaggcggt tcgttggcgg
      241 cgccgccgag agcagatttt tttgcatctg cattggcaga caatgcccac acggcagcgc
      301 tggtcatcag gttggcgcga gaaatatccc cgccaagtca gtaaaaacga caatgcggcc
      361 aattggcata acctccagac acccttcacg gcattggcag atcagatgac acttgtgatt
      421 tcagacagcg cggctcaagc cgatttgccc ctgggttggc tgggccggtt gccgccttgc
      481 ctgctttttt tgttgattgc caaagccaat ccggccattg acacaaagcg gcttgtcctg
      541 tcggcgcggg tcggctggac tgttggtcag gtcggcattc tcggtgtaca gcggtgtcat
      601 tgacagcccg caggtgctgt tgctggtggc acggcctgac catctcatca gcggccgccg
      661 cagggcaagg caattgcaac ggtatcgatg cggtgcgcac ggtgaggcac cggcaggttc
      721 gtgacgaggc tgccggtgtc atcagcgcag acttcattgt tgaccagcgc aggcgggctc
      781 aggatgccgt gctggtgccg ctgcaacagc cggtgcgtcg ggtcaagctg cgcggtagtt
      841 tgctgtgttg gttggttcag agcaacaatg cgatcaaaaa agctcagttc catctacccg
      901 gaacaaggta tcaaattaga ctttttttgc atgcacggca gtattctggc cttgtatttg
      961 ttaatttggg gatccagcct taaccggcca acgaaggttt tagaacccat gaattcgatt
     1021 gaagattcaa tgacacagcg gaccgagcga ctgcagcttc tcaagcagcg cgcccaggca
     1081 attttgaagc tgcacgatac tggctttggc gggggcgaat ctgacagttt gcaggtcgtc
     1141 aagctgctgg aagatctgcg cgtctaccaa gtcgagctgg aactgcaaaa cgaggagctg
     1201 cgcgaggccc agcaagaggc cgatctggcc cgcaaacgct accagaacct gtttacgaaa
     1261 atgcccatgc cggccctggt ggtggatgtc aatggcgtag tggacgactg caatgaactt
     1321 gccaacgcac acctcggcct gaaccggcgc aatcgggccg tcgacgcacg cttgtggcag
     1381 cggctcaagg gcaaggaccg agcccgcctg catgtggcat tgcgcgacct gttgcctggt
     1441 caaaccctgg tgctgccgca ggtcgtgatt ggtgaagcga atgctcaaac cccggttttt
     1501 gatgtgcaca tgatgggttt gtcaatcgac tacaggcttg atcatcgggt gttgctgatg
     1561 ctggtggatc gcaccgcaga aatacaacgt gaacaggatc accggtttta cgcctcgctg
     1621 cttgactcgg ccgacagcat catttacgcc gctgacaaag atgggcagtt gctgctggcc
     1681 aaccaggctt ttctcgactt gctgggtcgc cagcgcgatg aggtcaaggg cagcaagcgc
     1741 gaagccttcc tgccgctgcg cgatgccatt ttccacaacg aaaccgacca gaaagtgttg
     1801 gccaccggcg aagcgctgac agtccacgag cagtcttacc tggcggcaca gcgcggcgtg
     1861 acagacctgc tcacgcgcaa attcccttta cgtgacagca caggccgcat ttatggtgtc
     1921 ggtggcattt caaccgacat cacggcgctg aaggatcagc agcgccaggg gctgctgagc
     1981 gagaccgtgt tcatgcaggc acaagaggcc atcgtcatca ccgatgcatc aacccggatc
     2041 attcgggtca atccggcctt tgtcagacaa accggtttct ccctcgagtc caccgttggg
     2101 cataaaacca gcatgctcaa gtcggggcgg cagagccaga ttttttacca cgacatgtgg
     2161 cagtccatca ataacaccgg ttgctggtct ggcgagattg agaaccggcg cgccgatggc
     2221 gtttactaca cggtctggat caacatcaac gcggtgcacg accaagaccg aaagctgttg
     2281 tattacatcg gtgccagcac cgacattacc gagcatcgaa aggcccaggc cgagatacag
     2341 cgcagcgctg agctgctgcg cggcgccatt gatgtcattg atgaagcttt tgtgctgttt
     2401 gatgcagatg accggctggt gttttgcaat gacaagtacc gcagcctgta cgccacgtcc
     2461 aatgacctga tggtgcctgg cgcgcttttc atggatattg ttcgcgtcgg ggcagagcgt
     2521 ggccagttcc gtgatgcggt tggacgtgtc gatgaatggg tggcagagcg tatggcagcg
     2581 caccggagcg gcaattccag cacggtggtg cagttggaca atggccgggt gttgcgggct
     2641 gtcgagcgcc gcatgcctga cggtcacacc gtcggtttcc gcgccgacat taccgaattt
     2701 gtccgtgcaa ccgaagaggc aaaagcggcc aatctggcca aaagccgctt tcttgcgacc
     2761 atgagccatg aaattcgcac cccgatgaac ggcattttgg gcatggcgca gatgttgttg
     2821 atgcccagcc tgagtgccgc cgatcagctc agttatgtca acaccatact gtctagcggg
     2881 cagacgctgc tgtctttgct taatgacatt cttgacctgt caaagatcga agccggcaag
     2941 atccggctcg acagcgtcac gttcacgccg gacagcctgc tgggcgatat ctgcaacctt
     3001 ttttcaggtg cagcgcaagc caaaggactg gccatggact atcagtggct tggacccgcc
     3061 acacagtgct accaaagcga cgcttcacgc ttgcgacaga tgctctccaa cctggttggc
     3121 aacgcagtca agttcacctc attgggtaag gtacatctgc aaggcagcga aatcgagcga
     3181 caaggtgatt cggccctgct ggagtttgcc gtcactgatt ctggcatggg tatcgcagcg
     3241 gacaaactcg gcctgttgtt caaacctttt tcccagaccg acaactcgac cacccgtgaa
     3301 ttcggtggct ccggactggg cctgtcgatt gtcaacaatc tggccaaatc gatgaatggc
     3361 gatgtcggtg tgcaaagcga gctggggcgg ggttcgcggt tctggttcca ggtgcgggtc
     3421 acggtcatgc cagacggtgc cccattgccc ggcctgcaag gtcaggccac tgaaccggcg
     3481 gacctgcccg cattgtctgg gcgggtgctg gtggtcgagg acaacctggt gaactgcatg
     3541 gtgatcgaat cgctgctgaa gcggctgggc atggcggtca cggtggtcaa cgatggtcaa
     3601 caagccgtcg atgcgatcac cggcggtggt ccggacagtg aacccgacct gatcttgatg
     3661 gacttgcaga tgccggtgat ggacggctac accgcagtgc agcaaattcg ccagtgggaa
     3721 tcagctcagc aacgcccgcg ccacctcatc atcgccctga ccgccgatgc cttcgcagaa
     3781 gaccacctgc gttgcctggc cggcggcatg gatgacttcc tgaccaagcc gatcgctatc
     3841 gatgtgctga agaccgcgct ggtcaagtgg ctggtgccgg ttacagcaag gcattcgatt
     3901 ctgccgcaag gcacagcgcc gaattttgcc cggccgaatt gacgctgcca ggccgaccaa
     3961 cccagcccgc tcattgccac ccgactcaag tcggcctgcc aaagcgaccc aacgcggcat
     4021 tgacagacct gcgtcgggct gacttgtgct tgcattgcca aatcaatccg acatgaggtt
     4081 gcttcgcctt gccgggctgt agcactgcct gcggtttggc acccgctgtc gcatcggtct
     4141 gaaaacgcaa tcagacctgt cagttgggcg caggttgcat ccggccaagc gcgactttgc
     4201 ttgtcatttt gttgacaggc gatggcgctg taatgttcgg gtcgttcggt caaggtcata
     4261 aaaggcttcg ctgtggtatc cacttagaaa aaggcttttc catgtcaaat tacccctcca
     4321 attggcataa cgccagcctt ggcaccaagc tcgccatcag caactttgtt ttggtcgccc
     4381 ttgtcttctc ggtcttgatt gcagccattg cctattccgt taccagcgtg attgagtcac
     4441 gggcgaaagt tgaactcgaa gaccgaacca agctattggt ggaaatggtc agttcggccg
     4501 acaaggaact ccgcagtcga acctcggtgc tggccagggg ctttcagtcc agcctggctg
     4561 gcaagctcag tatcgacccc gccacagtgg aaattggcgg caaaccgaca cccacgttga
     4621 gcctcgacgg tcggccgctc aatctcgaat ttgcgctggt ggaccgattt accgctgcca
     4681 ccggtgccgt cgccaccgtg tttgccaaga ctggtgacga ttttgtgcgg atcacgactt
     4741 cgctcaagga cgacaagggc gaacgcgcga ttggcacgct gcttgaccgc gcgcaccctg
     4801 gctacaaggc cagcctgaca ggcaacagtt ttaccggact ggcaacgctg tttggtcgcc
     4861 aatacatgac ccagtacgac ccgatcaagg acgcgcaggg ccaggtcatc gggctaagtt
     4921 ttgtcggcct agatttcact gactatctgg tcaacctgaa aaacaccatc cgcagcctta
     4981 aagttggcaa ggagggcttt ttttatgtgc tggattcgcg ccccggcagc agctacggca
     5041 acctggtcat ccaccccagc gctgaaggca aaaatctatt gaatgtcagg gatgcagacg
     5101 ggcgggaatt tgccaaagac atgctggagc agaaaaatgg actgatgcgt tacgcgtggg
     5161 tcaacaagga actcggagaa accagcgcac gcgacaaggt ggtgtcattt tcttacctga
     5221 aaggctggga ctgggtgatt gccggtggca cctatgccga tgaatacgtg atcgaagtga
     5281 accagttgcg caatttgtat gtcttgctgg gcgtggtgct ggtgatcatc acctccggcg
     5341 tcttgttcct gttgatccgc cgcatcgtgc tggtgccgct ggcgcgggtg attggcgatg
     5401 cccaatcgat tgcacatggg gatttgagtg tgcagttgca aaccgaccgg cacgatgaaa
     5461 tcgggaagtt gatgaccgcc atgaacagca ttggcagcgg actcagcggc gtggttcagt
     5521 cggtgcgtac cggttccgag agcgtggcga ccgccagtgc cgagattgcc cagggcaacc
     5581 aggacttgtc ggcacgtacc gaacagcagg ccagcgcact ggagcagacc actgcgtcaa
     5641 tggaacagct cagcgccaac gtcaagcaaa atgccgacag cgcacgccag gccaacgcgc
     5701 ttgccatgag tgccacggat gtcgccatgc agggtggcac ggtggtcacc cagatggtcg
     5761 acatgatgaa aggcatcaat gccgcttcca acaagattgc cgacatcatt caagtgatcg
     5821 acggcattgc tttccagacc aacatcctgg ccctgaatgc tgccgtcgaa gcggcacggg
     5881 ctggcgatca gggtcgaggc tttgcggtgg tggccagtga ggtgcgcttg ctggctggtc
     5941 gcagtgccac cgcggccagg gaaatcaagg gcctgattga tgccagtgtc gagcaggtgg
     6001 tgcagggcaa cgcgctggca ggtcaggctg gcaacaccat gaccgaggtg gtcagcagca
     6061 tcaaacgggt gactgatctg atgggtgaga tcagtgccgc cagcgccgag caggccagcg
     6121 gtgtcgatca gatcggtgca gcggtgacgc acatggacca ggccacccag caaaacgcga
     6181 cgctggtgga ggaaatcgca gccgctgcca gcagtctcaa gttgcaggcc agcgagctgg
     6241 ttgaaaccgt cgcggtgttc aagctaagcg acgacaccgg gtatgccatc gccgccattg
     6301 gcaataccgc aggtagcagc gcagcggcgc agcgccggat cagcgcaact tgatggccga
     6361 ccgggggcac cagccagatc atcctaagtc ggacaagccg gaatcgatga gccttcgtcg
     6421 cgagggcgcg cctcctacac aaacagtcgg cgccaatttg aatttgtagg agtcgcgccc
     6481 tcgcggcgat tgactttgaa tgtttgaatt cttcgcatga tatgcaaaga cttgacttgt
     6541 tagcgcctaa ggatgtggtc gggaataact ccccgttttg caccaaggag ttggg
//
LOCUS       DAOSLL010000209         6594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10591_length_6594_cov_4.282459, whole genome shotgun sequence.
ACCESSION   DAOSLL010000209 DAOSLL010000000
VERSION     DAOSLL010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6594
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10591_length_6594_cov_4.282459"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="PLB25_10315"
     CDS             <1..235
                     /locus_tag="PLB25_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012251566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOE42006.1"
                     /translation="TTVALRALPVVILGGLTSVPGAIIGGLIIGVGEKLSEVFLGPYV
                     GGGIEIWFAYVLALLFLLYRPQGLFGEKIIDRV"
     gene            256..1332
                     /locus_tag="PLB25_10320"
     CDS             256..1332
                     /locus_tag="PLB25_10320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOE42007.1"
                     /translation="MFYRENGQFKTTYRADQQIFPITQDRWAVLLIIATAFIVVPMLT
                     SDYMFRAIFIPFLIMALAALGVNILVGYCGQISLGSGAFMAVGAYAAFNFFVRVPGMP
                     LLLALVLGGVCSAAFGILFGLPSLRVKGLYLAVATLAAQFFSDWMFLRIKWFTNDAPS
                     GSVSVSGLQVLGLPIDSPLSKYLLCLSILVVMALLAKNLVRSAIGREWMAIRDMDVAA
                     AVIGIRPMYAKLTAFAVSSFIVGVAGALWGFVYLGAWEPAAFSVDLSFKLLFMVIIGG
                     MGSIMGSFFGAAFIVVLPIFLNQFLPVLGGAVGVQISTVVTAHAELMIFGALIVWFLI
                     VEPHGLAKLWSIGKQKMRLWPFPH"
     gene            1412..2725
                     /locus_tag="PLB25_10325"
     CDS             1412..2725
                     /locus_tag="PLB25_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE42008.1"
                     /translation="MRVRNIAAAALIAALGTSAFAQAKEQFFPLLSYRTGAYAPNGVP
                     WANGKQDYIKMINARDGGINGVKITFEECETGYLTDRGVECYERLKSRPGVALFDPQS
                     TGTTFALTEKAPADKMPLMTLGYGLSVSQDGMAFKWNFPLMGSYWTGADILIQYIGKG
                     LGGLDKLKGKKITLVYHDSPFGKEPIPLLQERAKMHGFELQLLPVTAPGVEQKATWLQ
                     VRQSKPDFVFLWGWGVMNSTALKEAQATGFPRDKMYGVWWAGAEPDVKDVGEGAKGYH
                     SLALNTSGQQPKVIQDILKFVHGKGQGTGPKDEVGSVLYTRGVIIQMLSHEAVRRAQE
                     RFGKGKVMTSEQVRWGLENLSLDQKKLDALGFTGVMRPLSTSCADHMGSSAARIQTWD
                     GKKWEITSDFIEADEQILKPLIKAGSEKYLADKKTARRNAADCQS"
     gene            2835..3644
                     /locus_tag="PLB25_10330"
     CDS             2835..3644
                     /locus_tag="PLB25_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE42009.1"
                     /translation="MEPTNIVLNVNGIEVIYNHVILVLKGVSLKVPQGDIVAILGGNG
                     AGKTTTLRAVSNLLRGERGEVTKGSIELRGERIENLSPADLVQRGVVQVMEGRHCFAH
                     LTIEENLLTGSYTRRDKGEIAANLDKVYSYFPRLKTRRSSQAAYTSGGEQQMCAIGRA
                     LMTNPSMVLLDEPSMGLAPQIVEEVFEIVKDLNGKEKVTFLLAEQNTNMALKYADYGY
                     IMESGRVVMDGIAKDLANNEDVKEFYLGMGGGQRKSFKDVKSYKRRKRWLA"
     gene            3790..5046
                     /locus_tag="PLB25_10335"
     CDS             3790..5046
                     /locus_tag="PLB25_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE42010.1"
                     /translation="MTYFFDSLETRHPDQREAELLSALPAQVAHAKLSCLAFAEALKD
                     VNPMLTNTRAALTKLPVIRKHELLELQLASRSRGASVFGGFSAIGFGASMPRVFASPG
                     PIYEPEGTAKDYWRMARAIFAAGFRPGDLIHNSFSYHFVPAGSMMETGAHALGCSVFP
                     GGTGQTEQQVQAMAELKPAGYIGTPSFLKIILEKATEMGVALPSVTKAMFGGEAFPPS
                     LRDWFTAHGVAGYQCYATADLGSIAYETSAREGLVVDEGVLLEIVRPGTGDPVAEGEV
                     GEVVVTTLNPAYPLIRFGTGDLSAVLPGHCPSGRTNTRIRGWLGRADQTTKIRGMFVH
                     PGQVDQIAKRFPEVGRARLVVTGEMANDSMTLKVETACSGPDALSAKLMEAIRDVTKL
                     RGQVEIVLPGSLPNDGRVIEDARSYK"
     gene            complement(5398..>6594)
                     /gene="rmuC"
                     /locus_tag="PLB25_10340"
     CDS             complement(5398..>6594)
                     /gene="rmuC"
                     /locus_tag="PLB25_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HOE42011.1"
                     /translation="FEVRIQERFEALSGATRQTLDSLKADINNQLAVMSGTLKDQLDG
                     NSGQIRNQFSALQASVSQQLTALVAGSQQNAEQLRVALNERLAAIQLDNTTKLEEMRR
                     TVDEKLHATLEQRLGESFKLVSERLEQVQAGLGEMKTLAGSVGDLKRVMTNVKSRGTW
                     GEMQLGAIIDNVLTMDQYGKNVKTVPGSDELVEFAIRLPGKHEDHPIWLPIDCKYPVE
                     HYQRLMDAQDAMDKVAILQAGNAFETSMKLEARKIVAKYVSPPHTTDFAILYLPTEGL
                     FAEVIRRPGLVEAIQNDCRVMITGPANLAAMLNSLQMGFKTLAIEKRSSEVWSLLGMV
                     KTEFSKFGEIVEATKKSIDAAASKFNEVGVRTRAIQRRLRDVEELPTQVVPPLPDTVV
                     EVLAQQ"
BASE COUNT         1269 a         1883 c         2000 g         1442 t
ORIGIN      
        1 caccacagtc gcgctgcggg cgctgccggt ggtgattctg ggtggtttga cctcggtgcc
       61 gggggccatc attggcggcc tgatcattgg tgtcggcgaa aaattgtcgg aagtgttttt
      121 gggcccgtat gtcggaggcg gcatcgaaat ctggtttgcc tacgtgctgg ccctgctgtt
      181 tttgctgtac cggccacaag gcctgtttgg cgaaaagatc attgaccgtg tgtaacaact
      241 gaacaaggca acgacatgtt ttacagagaa aacggccaat tcaagaccac ctaccgcgcc
      301 gatcagcaga tctttccgat cacgcaggac cgctgggcgg tgttgctgat catcgccacc
      361 gcttttattg tggtgcccat gctgaccagc gactacatgt tccgggccat tttcatcccg
      421 tttttgatca tggcgctggc cgccctgggg gtcaacattc tggtcggcta ctgcggccag
      481 atctcgctgg gttccggtgc tttcatggcg gtcggcgctt atgccgcgtt caattttttt
      541 gtccgggtgc ccggcatgcc gctgctgctg gcgctggtgc tcggcggggt ttgctcggcc
      601 gcttttggca tcctgtttgg cttgcccagt ttgcgcgtca agggcttgta cctggcggtg
      661 gcgacactgg cagcgcagtt tttcagcgac tggatgtttc tgcgcatcaa gtggtttacc
      721 aatgacgcgc cgtccggctc ggtgtcggtg tcgggcctgc aggtgctggg tctgccgatt
      781 gacagcccgt tgtccaaata cctgctgtgt ctgtccattc tggtggtgat ggcgttattg
      841 gcaaaaaacc tggtgcgcag cgccattggc cgcgagtgga tggcgattcg tgacatggat
      901 gtcgccgccg cagtgattgg cattcgcccg atgtatgcca agctgactgc ttttgcggtg
      961 agttccttca ttgtcggtgt ggccggcgcg ctgtggggct ttgtctatct gggggcctgg
     1021 gagccagcgg cattttcggt ggacctgtct ttcaagctgc tgttcatggt catcattggc
     1081 ggcatgggtt ccatcatggg cagctttttt ggcgcggcat tcattgtcgt gctgccgatt
     1141 tttctgaacc agttcctgcc agtgctcggc ggcgcggtcg gggtgcagat ttctacggtt
     1201 gtgacggcgc atgccgaatt gatgattttt ggcgcgctga tcgtctggtt cctgattgtc
     1261 gagccgcacg gtctggccaa actctggtct atcggcaagc aaaaaatgcg tctttggcca
     1321 tttcctcact gagtttggac agttgttttt cgtcagttgg ttggtatgta aatcccggca
     1381 ctgcccagtg ctatctttct aggagacaaa tatgagagtc agaaacattg ccgcagcagc
     1441 cctgattgcc gcgctcggca ccagcgcctt tgcccaggcc aaggagcagt tctttccgct
     1501 gctgtcgtac cggaccggtg cttacgcgcc caacggcgtg ccatgggcca atggcaagca
     1561 ggattacatc aaaatgatca acgcgcgcga cggcggcatc aacggtgtca agatcacttt
     1621 tgaagagtgc gaaaccggct acctgaccga ccgtggtgtc gagtgttacg agcgcctcaa
     1681 gagcaggccc ggcgtggcgc tgtttgatcc gcagagcacc ggcacgacct ttgccctgac
     1741 cgaaaaggca ccggctgaca aaatgccctt gatgacgctg ggttacggcc tgtcggtgtc
     1801 gcaggatggc atggcattca agtggaattt cccgctgatg ggcagctact ggaccggggc
     1861 cgatattctg atccagtaca tcggcaaggg cctgggtggt ctggacaagc tcaagggcaa
     1921 gaaaatcacc ctggtctatc acgacagtcc gtttggcaag gagccgattc cgctgctgca
     1981 agagcgtgcc aagatgcacg gtttcgagtt gcaactgctg ccggtgaccg cccccggtgt
     2041 cgagcaaaag gccacctggc tgcaggtgcg tcaatccaag ccagactttg tcttcctgtg
     2101 gggctggggc gtgatgaatt ccaccgccct caaagaggca caggccaccg gtttcccgcg
     2161 tgacaagatg tacggtgtct ggtgggccgg tgccgagccg gacgtgaaag acgtgggtga
     2221 aggtgccaag ggctatcact cactggcgct caatacctcg ggtcagcagc ccaaagtcat
     2281 tcaggacatt ctgaaatttg tccatggcaa agggcagggc acagggccca aggacgaagt
     2341 tggctcggtg ctgtacaccc gtggcgtgat catccagatg ctgtcgcatg aggcggtgcg
     2401 ccgtgcccag gaacgttttg gcaagggcaa ggtcatgacc agcgaacaag tacgctgggg
     2461 cctggaaaac ctgtcgctgg accagaaaaa gcttgacgcg ctggggttca ccggtgtcat
     2521 gcgccccttg agcacctcgt gtgctgacca catgggttca agtgccgccc gcattcaaac
     2581 ctgggacggc aaaaagtggg agatcacctc agacttcatc gaggctgacg agcagattct
     2641 caaaccgttg atcaaggccg gctcggaaaa atacctagct gacaagaaaa ccgcccggcg
     2701 caacgccgca gactgccagt cctgatcgct gcctgactat cagttcttcc actgtcatgc
     2761 cggacccggt ctggcctgac agtccaacag gcagcattgc gtgcagtgct gcgtattggt
     2821 gaaagcaagg aaatatggaa cccacaaaca tcgttctcaa cgtcaatggc atcgaggtca
     2881 tttacaacca cgtcattctg gtgctcaagg gtgtttcgct gaaggtgccg cagggcgaca
     2941 tcgtggcgat tctgggcggc aatggcgcgg gcaagaccac cacactgcgg gcggtgtcca
     3001 acctgctgcg gggtgagcgc ggcgaggtca ccaagggttc catcgagttg cgcggcgagc
     3061 gcatcgaaaa cctgtctccg gccgacctgg tgcagcgcgg cgtggtgcag gtgatggaag
     3121 gccgccactg ctttgcccac ctcaccatcg aagaaaacct gctcaccggc agctacacgc
     3181 gccgcgacaa gggcgagatt gccgccaacc tggacaaggt ttacagctat ttcccacgcc
     3241 tcaaaacccg tcgcagcagc caggccgctt acacctcggg aggtgagcag caaatgtgcg
     3301 caatcggccg ggccttgatg accaacccca gcatggtgct gctcgacgag ccatccatgg
     3361 gcctggcacc gcagattgtt gaagaggtgt tcgagatcgt caaagacctt aacggcaagg
     3421 aaaaagtgac ctttttgctg gccgagcaaa acaccaatat ggcgctgaaa tacgccgact
     3481 acggctacat catggaaagt ggccgggtgg tgatggacgg catcgccaag gacctggcca
     3541 acaacgaaga cgtgaaagag ttttaccttg gcatgggtgg tggtcagcgc aagagcttca
     3601 aggatgtcaa aagctacaag cgccgcaaac gctggctggc ctgatggtcg gcttgtcatg
     3661 ccggacctga tccggtaccc ggcgctgctc gaagccgtcg attgccgtgc tttgcacacc
     3721 gcgccagatc gtgcaacccc agatgcccgc aacttttttt gtgccaattg acttacgtgg
     3781 aacccagcca tgacttactt tttcgactcc cttgaaaccc gccatcctga ccagcgcgag
     3841 gctgaactgc taagcgcctt gcccgcacaa gtggcgcatg ccaagttgtc ttgcctggct
     3901 tttgccgagg cactgaaaga tgtgaacccg atgttgacca acacccgggc tgctttgacc
     3961 aaattgccgg tgatacgcaa gcatgagctg ctggaactgc aactggccag ccgcagccgt
     4021 ggtgccagcg tgtttggcgg ctttagcgcg attggctttg gtgccagcat gccacgcgtg
     4081 tttgccagtc cggggccgat ttacgagcca gagggaaccg ccaaagacta ctggcggatg
     4141 gcacgggcga tttttgccgc cggttttcgg cccggtgatc tgatccacaa cagcttcagc
     4201 taccactttg taccggctgg ctcaatgatg gaaaccggtg cccatgcgct gggttgcagt
     4261 gtgtttccgg gcggtaccgg ccagaccgag cagcaggtgc aggcgatggc cgaactcaag
     4321 cctgctggct acattggcac accgagtttt ctgaaaatca ttctggaaaa agccaccgag
     4381 atgggcgtgg cattgcccag cgtcaccaag gccatgtttg gcggcgaagc ttttccgccc
     4441 agcctgcgcg actggtttac cgcacacggc gtggcgggtt accagtgcta cgcgacggcc
     4501 gatctgggct caattgcgta tgaaaccagc gcgcgcgagg gcctggtggt ggacgaaggc
     4561 gtgctgctgg agattgtgcg ccccggcacc ggcgacccgg tggccgaggg tgaagtcggt
     4621 gaggtggtgg tgaccacgct caatccggct tacccgctga tccgttttgg caccggcgat
     4681 ctgtcggcgg tgttgccggg ccactgcccg agcgggcgca ccaacacccg catccggggc
     4741 tggttgggcc gcgctgacca gaccaccaag atccgtggca tgtttgtcca tccgggccag
     4801 gtcgatcaga ttgccaagcg attcccggaa gtgggtcggg cgcggctggt cgtcaccggc
     4861 gaaatggcca acgacagcat gacgctgaag gtcgaaaccg cctgcagcgg gccagatgcg
     4921 ctgtcggcca aattgatgga agcgattcgt gacgtgacca aactgcgcgg ccaggttgaa
     4981 attgtcttgc ccggttcgtt gcccaacgac ggtcgggtga tcgaggacgc gcgcagttac
     5041 aaatgagcat cccaaccccg cacgcgggcc gggcgttgct aattgaacat ggggttgagg
     5101 ctgatgccac aggcactgca agccgcccag cttttatatg ccaaatcggg ttacatccct
     5161 tatgcaataa gcgttgccag ctactgtttt gattgtcaac gcggtatctg ttgcaggtaa
     5221 agcttcagcg ggcggcagcg gcaccatccg gcagattgcc agacgctgca tgactgaagt
     5281 ttgctccaaa caaatgcaac tgccgggcgg cagatatggc aatgctggtg atcacatgtt
     5341 gttgggctgc cccatgccaa agcaggtttg ctgcaacaag ctgacgcttg cactgaatta
     5401 ctgctgtgcc agtacctcta ccaccgtgtc aggcagcggt ggcacaacct gcgtcggcag
     5461 ctcctccaca tcgcgcagcc gcctctgaat tgcccgggtg cgcacgccaa cctcgttgaa
     5521 tttgctggca gccgcgtcaa tcgacttttt ggtcgcctcc acaatctcgc caaacttgct
     5581 gaactcggtt ttcaccatgc ccagcaggct ccagacctca gatgagcgct tttcaatcgc
     5641 cagcgtcttg aagcccattt gcaggctgtt gagcatggcg gccaggttgg ccgggccggt
     5701 gatcatgacg cggcagtcgt tttgaatcgc ctcaaccagg ccagggcggc ggatcacctc
     5761 ggcaaacagg ccctcggttg gtaggtacaa aatggcaaag tcggtggtgt gcggcggtga
     5821 cacatacttg gcaacgatct ttctggcctc gagtttcatc gaagtctcaa aggcgttgcc
     5881 ggcctgcaga atcgccacct tgtccattgc gtcctgcgcg tccatcaggc gctggtaatg
     5941 ttccaccggg tatttgcagt cgattggcag ccagatcggg tggtcttcat gcttgcccgg
     6001 caggcggatg gcaaattcga ccaactcgtc gctgcccggc acggtcttca cgtttttgcc
     6061 atattggtcc atcgtcagca cgttgtcgat gatggcgccg agctgcattt cgccccaggt
     6121 gccgcgcgat ttgacgttgg tcatcacccg tttgaggtca cccacgctgc cagccagggt
     6181 cttcatctcg cccagaccgg cctgcacctg ttccagccgc tcactgacca gcttgaacga
     6241 ttcgcccagg cgctgttcca gcgtcgcgtg caatttttca tcgacggtgc ggcgcatttc
     6301 ttcgagcttg gtggtgttgt ccaactggat cgcggccagc cgctcgttga gtgcaacccg
     6361 caactgctcg gcgttttgct ggctgccagc caccagagcg gtgagctgct gcgacaccga
     6421 cgcttgcagc gcagaaaact ggtttctgat ctgaccactg ttgccgtcca actggtcttt
     6481 caaggtacca gacatgacgg cgagctggtt gttgatgtcc gccttcaacg aatcgagcgt
     6541 ttgccgggtg gccccgctca aggcttcaaa gcgctcctga atgcgcactt cgaa
//
LOCUS       DAOSLL010000210         6592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10599_length_6592_cov_4.884045, whole genome shotgun sequence.
ACCESSION   DAOSLL010000210 DAOSLL010000000
VERSION     DAOSLL010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6592
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10599_length_6592_cov_4.884045"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PLB25_10345"
     CDS             <1..288
                     /locus_tag="PLB25_10345"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOE42012.1"
                     /translation="AVLPVVRLRSLYNIESTLPERTSVVVVNSPRGKYGIEVEVLLGQ
                     QQTVIKPLGRLFKTLRGISGSSILGSGEVALILDVSSLGDLVTAEAATTSL"
     gene            334..744
                     /locus_tag="PLB25_10350"
     CDS             334..744
                     /locus_tag="PLB25_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42013.1"
                     /translation="MKKIVTYVCFVMLALLAIPWAHADDFKDLRAKMTVARETLVAMA
                     TDKTKRGADQQKIVKDTADAVSAMLAKFKAPAGKEGQFKELVEAWGAFKKTRETELVP
                     LILAGKDDEALKLAGGIQKERINKCMSLVGELGG"
     gene            815..3349
                     /locus_tag="PLB25_10355"
     CDS             815..3349
                     /locus_tag="PLB25_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE42014.1"
                     /translation="MKDLKIGNRLSILLALLVLLLLITGWVGLRATREANAKMENQYA
                     NGMLPLEYMSNIEALMQANQLLVLRAITNPSVENSKRAVEGVTANIDTISKSLKLFQA
                     TAKTEAETKLVADFEVKRTAFVKDGLTPMVDALRDNSPARAGLVDETLTNRWAAVVPV
                     MQSIKKLQLEKSRADYEESLVSASATQKSIILIILAALGLTVVAGYFLIRSITVPLRH
                     AVTVAEDVAVGKLDANITVDRGDEVGHLLGAMSTMQGVLSQFMDAQNEMTRQHDSGML
                     DYVMPTDHLQGAYRSMTEAVNRLVQSHIAVKMKVVEVVTAYTEGHLDVAMDRLPGQKA
                     RITAALDLVQTAMKQASEAAAFNQRIRLSLDSLPVCVTVSNAQAMLVHATPQAKELLK
                     LFGGASFDTDKFYGNKLSTLFKDPVHATRFDQAVRSGETVDMEVNGHHLRLLARPVLD
                     GRGTPLGRITQWIDRTDELASEQELDDIVNAATQGDFSGRLALQGKNGFFGKIATGMN
                     QLMSTSEQGLSDVAQVLSSVAEGDLTQRITRDYAGLFGKVKDSVNTSSDNLTRVIGEV
                     RAAADALTGAANQVSATAQSLSQAASEQAASVEETTSQIDVMSSSINQNSDNAKITDG
                     MATKTNKEAIEGGAAVSLTVEAMKKIAAKIGIVDDIAYQTNLLALNAAIEAARAGEHG
                     KGFAVVAAEVRKLAERSQEAAKEIGELAGNSVTTAERAGKLLDEIVPSIQKTSELVQE
                     IAAASTEQSESVTQIGGAMGQLSKATQQNASASEELAATSEELSGQAEQLQQSIAFFN
                     TGDGAQPVRSRHATSALERRVPQARATAPLRVAAVRQSDSAHFKPY"
     gene            3359..3901
                     /locus_tag="PLB25_10360"
     CDS             3359..3901
                     /locus_tag="PLB25_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOE42015.1"
                     /translation="MSSHHVVAAPSDSQPVFRRDSVKDEALQYLTFSLGDEVFAMDIR
                     SVREIIQYGSMTIVPLMPEFVRGVVNLRGAVVPVIDLKSRFGRPPAQMGKKTCVIIFD
                     VGPEGDRVELGLLVDAVSEVIDIAPSSIEPPPQFGTSIAREYIHGLGKVGDEFIVILE
                     PERALNIDDMAALAEQGHRV"
     gene            3955..6528
                     /locus_tag="PLB25_10365"
     CDS             3955..6528
                     /locus_tag="PLB25_10365"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE42016.1"
                     /translation="MKQLSIKTRILVGFALPIALFIGFTFWFSAQLSQVRQSMNHVSE
                     QSVQYALLATVLDKNVVQIQQFLSDISATQGKDGLDDGFQRAQANYDEFRVGLAKFEK
                     HFAEVGDAVSAKQVQTIKADAADYYAAGQTMAQAFVAAGPMAGNKLMGGFDAASEKLQ
                     KQLEPFVKAQVEQMKADLAASSARTDQVSRIGIVIVAVAVVLALLVAFAVTASITRPL
                     ARALAAARQVAAGKLDTEIDADDSEIGQLMAPLAKMKDTLLQFEAAQTELARQHDLGM
                     LDYRMPLNQLEGDYKAMGQSINTLVNSHIAITLKVVDVVAAYAGGRFDVAMDRLPGQK
                     ARISEALDKVQNALSEAATAAQRNQRIKVALDNCSTNVMIADTDFNIVYMNDTVQAMM
                     QRNEAELRKSLPRFEARHLIGQNIDLFHKNPGHQRGLLSGLKSGYKTQIQVGKLYFAL
                     SANPILDPSGERLGTVVEWFDRTAEVHIENEVASVVEHAVVGDFQHRLSTQDKTGFFA
                     SLSAGMNQLLEVSEQGLSDVAQVLSAVAQGDLTQRITRDYAGLFATVKESVNTSSENL
                     TRVIGEVRAAADALTGAANQVSATAQALSQAASEQAASVEQTTESVKIMSSSINQNSD
                     NAKVTDGMATRTNKEAVEGGAAVSLTVSAMKQIASKIGIVDDIAYQTNLLALNAAIEA
                     ARAGEHGKGFAVVAAEVRKLAERSQEAAKEIGELAGNSVTTAERAGKLLDEIVPSIQK
                     TSELVQEIAAASSEQSESVTQIDGAMGQLSKATQQNASASEQLAATSEELSGQAEQLQ
                     QSIAFFNTGAGPQPVRSRHELPAPALPTPSRKSIGTGRTATPLIATPTRKGGSGNFKP
                     Y"
BASE COUNT         1556 a         1874 c         1942 g         1220 t
ORIGIN      
        1 gcggtgctgc cggtggtgcg attgcgttcg ctctacaaca ttgaatcgac gttgccagag
       61 cgcaccagtg tggtcgtggt caactcgcca cgtggcaaat atggcatcga ggtggaggtg
      121 ctgctgggcc agcagcaaac cgtcatcaaa ccattgggtc ggctatttaa gacgctgcgt
      181 ggtatttcag gctccagcat tctgggcagt ggcgaggtgg ctctgattct ggatgtcagt
      241 tccctgggtg atttggtaac ggccgaagcg gcaactacca gtttgtgagt cgactcaaaa
      301 tgccgtgttt ttcttacttg aagggataaa gctgtgaaaa agatagttac ttatgtatgt
      361 ttcgtcatgc tggcattgct ggcgatacct tgggcgcatg ccgacgattt caaagacctg
      421 cgtgccaaaa tgacagtggc tcgcgagacc ctggttgcca tggccaccga caaaaccaag
      481 cgcggggctg accagcaaaa aatagtcaaa gacaccgccg atgctgttag cgccatgctg
      541 gccaaattca aggccccggc gggcaaagag ggccagttca aggaactggt tgaagcctgg
      601 ggagccttca agaagacgcg cgaaacagaa ttggttcccc tgattctggc cggcaaagat
      661 gacgaagcgc tgaagcttgc cggtggcatt caaaaggagc gaatcaataa gtgcatgagc
      721 ctggtgggtg aacttggtgg ctaaaaaagc gcgctgaggt gtgctgcgcc cccataggtg
      781 gcgcaatgga tttgactgga gaacgggtat gggtatgaag gacttgaaaa tcggaaatcg
      841 tttgtccatt cttttggccc tgctggttct cctgctgctg atcactggtt gggttggctt
      901 gcgtgcaacc agggaggcca acgcaaaaat ggagaaccag tatgcgaacg gcatgctgcc
      961 tcttgaatac atgtccaaca tagaagccct gatgcaggcc aatcagttac tggtgttgcg
     1021 ggccatcacc aacccttcgg tcgaaaattc aaagcgtgcg gtggagggtg tcacggccaa
     1081 catcgacacc atcagcaaat cgctcaagct gttccaggcc acagcaaaaa ccgaggcgga
     1141 gacaaagctg gtcgcggatt tcgaggtcaa acgcacggcg tttgtcaaag atggcctgac
     1201 ccccatggtt gatgcactca gggacaacag cccggcgcgg gccggactgg ttgacgaaac
     1261 actcaccaac cgttgggcag cggtggtacc ggtcatgcaa agcatcaaaa agctccagct
     1321 tgagaagtca agagccgact atgaggagtc cctggtcagc gccagtgcca cgcaaaagag
     1381 catcattctg atcattttgg cagcacttgg actcactgtc gtggccggtt actttttgat
     1441 ccgaagcatc accgttccac tgcgtcatgc ggtgacggtg gccgaagacg tggccgtcgg
     1501 caaactcgat gccaatatca cggttgatcg gggtgacgaa gtcggccatc tgctgggtgc
     1561 catgtccacc atgcagggcg tactcagcca gtttatggat gcgcaaaacg aaatgacccg
     1621 ccagcacgac agcggcatgc tggactacgt aatgccgacc gaccatttgc aaggagctta
     1681 ccggtccatg accgaagccg tcaatagatt ggtgcagtca cacattgccg tcaagatgaa
     1741 agtggtggag gttgtgaccg cctacaccga aggccatctt gatgtcgcca tggaccgctt
     1801 gccaggccaa aaagcccgca tcaccgctgc gctggacctg gtgcaaaccg ccatgaagca
     1861 agcgtcagag gcggccgcct tcaaccagcg catccgactg tcacttgaca gcctgccggt
     1921 ctgcgtgacg gtgtccaatg cgcaggccat gctggtacac gcaacaccgc aggccaaaga
     1981 gttgttgaag ctatttggcg gtgccagctt tgataccgac aagttctatg gcaacaagct
     2041 cagcactctc tttaaagacc cggtccacgc tacccgcttt gaccaggcgg tgcgctccgg
     2101 ggagacagtg gacatggagg tgaatggcca ccacttgcgc ttgctggcgc gaccggtgct
     2161 cgatggtcgg ggtacgccgc tggggcgcat cacgcagtgg attgaccgca ccgatgaatt
     2221 ggcatcggag caagagcttg acgacatagt caacgccgcc actcagggcg atttcagcgg
     2281 ccgtttggcg ctacagggaa aaaacggctt tttcggcaag atcgccaccg gcatgaacca
     2341 gttgatgagc accagcgaac aaggcctgtc ggatgtcgcg caggttttgt cgtcggtggc
     2401 tgaaggggac ctgacccaac gcatcacgcg cgactacgcc ggtctatttg gaaaagtcaa
     2461 agacagcgtc aacaccagca gcgacaacct gactcgcgtt atcggcgaag tgcgcgcagc
     2521 cgccgatgcc ctgaccggtg ccgccaacca ggtgtcagcc acagcgcaat ccttgagcca
     2581 ggcggccagc gagcaagctg ccagcgtgga agaaaccacc tcgcaaattg atgtcatgtc
     2641 aagctcgatc aaccagaaca gcgacaacgc caaaatcacc gacggcatgg caaccaagac
     2701 aaacaaagag gccattgagg gtggagccgc cgtcagtctt actgttgaag ctatgaagaa
     2761 aatagcagcc aagattggca tcgtcgatga catcgcctac cagaccaacc tgctggcgct
     2821 gaacgccgcg atcgaagcgg ccagagccgg tgagcacggc aagggttttg cagtggtggc
     2881 agccgaggtg cgcaagctcg ccgagcgcag ccaggaagct gccaaggaga tcggtgagct
     2941 cgctggcaac agcgtcacca cggctgagcg ggccggcaaa ctgctcgacg agattgtgcc
     3001 cagcatccag aagaccagtg aattggtgca ggaaattgcc gccgccagca ccgagcaaag
     3061 cgaatcggtg acccagattg gcggtgccat gggccagctt tccaaagcca cgcaacaaaa
     3121 cgccagtgcc tccgaagaac tggccgccac cagtgaagag ttgtccggcc aggccgagca
     3181 attgcaacaa agcattgctt ttttcaatac cggagacggc gcgcagccgg tgcgcagccg
     3241 acatgccacg tctgcattgg agcgccgtgt gccgcaggcc cgtgccacag caccgctgcg
     3301 ggttgcagcc gttcgccaga gcgattcagc tcatttcaag ccttattgag gtgccaagat
     3361 gtcaagtcac catgtcgttg ctgctccctc tgactcgcaa cccgtgtttc gccgggattc
     3421 ggtcaaggac gaggcgttgc aatacctgac gttcagtctg ggcgatgaag tctttgccat
     3481 ggacatccgc agtgtgcgcg aaatcattca gtatggctcc atgaccatcg tgccgctgat
     3541 gcccgaattt gtccgtggtg tcgtcaacct gcgaggtgcg gtggtgccgg tgattgacct
     3601 gaaatcgcgt tttggccgtc cgccggccca aatgggcaaa aaaacctgcg tcatcatttt
     3661 tgatgtcggt cccgaaggcg accgggtcga actcgggctg ctggtggatg cggtcagcga
     3721 ggtgattgac attgccccgt ccagcattga gccaccgccg cagtttggca cttccatcgc
     3781 gcgcgaatac attcacggct tgggcaaggt gggcgacgag ttcattgtca ttttggagcc
     3841 agaacgtgca ctcaacatcg atgacatggc ggcgctggct gagcaaggcc atagagtctg
     3901 agccctgacg attgacagca acacgtcacc aaacaacaca agatggcaac cccaatgaaa
     3961 caactatcta tcaagacacg catcttggtc ggctttgcat tgcccattgc gctgttcatc
     4021 ggctttactt tctggttttc tgcacagctt tcacaggtca ggcagagcat gaaccacgtc
     4081 tcagagcaaa gtgtgcaata tgccttgctg gccaccgtcc tggacaagaa cgtggttcaa
     4141 atccagcagt tcctgtctga catctctgcc acccagggca aagacggcct ggacgatggg
     4201 tttcagaggg cgcaagccaa ctatgacgag ttcagagtcg gcctggccaa attcgagaag
     4261 cactttgccg aagtgggcga tgcggtttct gccaaacagg tgcaaaccat caaggccgat
     4321 gccgccgatt actacgcagc cgggcagacc atggcccagg cctttgtggc ggccggtccg
     4381 atggctggaa acaagttgat gggcggcttt gatgctgcca gcgaaaagct gcaaaagcag
     4441 cttgagccct ttgtcaaggc ccaggtcgaa cagatgaagg cagatttggc ggcttcttca
     4501 gccaggaccg accaggtttc ccggattggc atcgtcattg tggcggttgc ggtcgtgctg
     4561 gctttgctgg tggcctttgc ggtgacggcc agcatcaccc ggccgctggc gcgtgcgctg
     4621 gctgccgcgc gccaagtcgc cgcaggcaag ctcgacaccg aaatcgatgc cgacgacagc
     4681 gaaatcggcc agctcatggc gcccttggcc aagatgaaag acaccctgtt gcaatttgaa
     4741 gccgcgcaaa ccgagctggc gcgccagcat gatctgggca tgctggacta ccggatgcct
     4801 ttgaatcaac tcgaaggtga ctacaaggcc atggggcagt ccatcaacac cctggtgaat
     4861 tcacacattg ccatcacact gaaggtggtt gacgtggtag cggcttatgc tgggggccgg
     4921 tttgatgtcg cgatggatcg cctgcccggg cagaaagcac gcatcagcga ggcactggac
     4981 aaggtgcaaa acgcgctcag tgaggcggca accgccgctc agcgcaacca gcgcatcaag
     5041 gtggcactcg acaactgcag caccaacgtg atgattgccg acaccgactt caacatcgtt
     5101 tacatgaacg acacggtgca agccatgatg cagcgcaacg aggctgaatt gcgcaaatct
     5161 ttgcccaggt ttgaagcgcg tcacctgatc ggccaaaaca tcgatctgtt tcacaaaaac
     5221 ccggggcatc agcgtggcct gctcagcggg ctgaaatcgg gctacaagac ccagatccag
     5281 gtgggcaaac tctattttgc gctgtccgcc aaccccattc ttgaccctag cggagagcgg
     5341 ctgggtaccg tggtcgagtg gtttgaccgt accgccgaag tgcatattga aaacgaagtt
     5401 gccagcgtgg tcgagcatgc tgtggtcggc gactttcagc accgcctgag cacgcaggac
     5461 aagaccggtt ttttcgccag cctgtcggct ggcatgaacc agttgctaga ggtcagcgaa
     5521 caaggtctgt cagacgtggc acaggtgctt tcagcggtgg cccagggcga cctgacccag
     5581 cgcatcacgc gcgactatgc cggcctgttt gccacggtca aggaaagcgt caacacctcg
     5641 tcggagaacc tgacccgcgt tatcggcgag gtacgcgccg cagccgatgc actgaccggt
     5701 gctgccaacc aagtaagtgc caccgcccaa gccctcagcc aggccgccag cgaacaagcc
     5761 gccagcgtcg agcagaccac cgagagcgtc aaaatcatgt caagctcgat caatcaaaac
     5821 agcgacaacg ccaaagtcac cgatggcatg gccacccgga ccaataaaga agccgttgag
     5881 ggtggagccg ccgtcagtct taccgtgtca gctatgaaac agatagcatc caagatcggc
     5941 atcgtcgatg acatcgccta ccagaccaac ctgctggcct tgaacgccgc cattgaagcg
     6001 gccagagccg gtgagcacgg caagggtttt gccgtggtgg ccgccgaagt tcgcaaactg
     6061 gccgagcgca gccaggaagc tgccaaggag atcggcgaac tcgcgggcaa cagcgtgacc
     6121 acggccgagc gggccggcaa actgctcgat gaaattgtgc ccagcatcca gaagaccagt
     6181 gagctggtgc aggagattgc tgcggccagc agcgagcaaa gcgagtcagt gacgcaaatc
     6241 gacggtgcca tggggcagtt gagcaaagcc acgcagcaaa acgccagtgc ctcggaacaa
     6301 ctggccgcca ccagcgaaga gctgtccggc caggccgaac agttgcaaca aagcattgcg
     6361 tttttcaaca ccggcgctgg cccgcaacca gtgcgcagcc gtcacgaatt gcctgcaccc
     6421 gcgctgccca cgccctcgcg aaaaagcatt ggcacgggtc gcaccgccac gccgctgatc
     6481 gcaacgccta cgcgcaaggg cggcagcggc aacttcaaac cgtattgatg gctggcttga
     6541 aagccagatc aaaaggacac aaatcgcatg aaaaacaagc aaacgcctac cc
//
LOCUS       DAOSLL010000211         6590 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10606_length_6590_cov_4.975669, whole genome shotgun sequence.
ACCESSION   DAOSLL010000211 DAOSLL010000000
VERSION     DAOSLL010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6590
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10606_length_6590_cov_4.975669"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1387
                     /locus_tag="PLB25_10370"
     CDS             <1..1387
                     /locus_tag="PLB25_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOE42017.1"
                     /translation="DLFAYDELTRLLKRNISVAAVPESQLALAFDRLYRRTEEISGLA
                     RALEKDLGDAVDFGELTATVGTEGAPVVRLLQSLFEDAMQVGASDVHIEPQEAGLQIR
                     VRVDGVLQTQTQADKRIGGALAQRLKLMAGLDISEKRLPQDGRFSVRLRDITIDVRLS
                     TLPTNYGESAVMRLLKQGSGMKRLDDIGMPADMLKRFREVLGRSSGLVLVTGPTGSGK
                     TTTLYAALAEINAGELKVITVEDPVEYRLPGLTQVQVNDKIELTFARVLRACLRQDPD
                     VILVGEMRDPETAEIGLRAAITGHLVLSTLHTRDAISTPFRLLDMGVPPFMVATSLQA
                     VIAQRLVRLNCTDCAEPHEPSPQEQSWLVGMLPAGGTVSARRGRGCSSCNGTGYSGRQ
                     GIYELLEMDAGLTQAASRSDPAAFMKLARDRMKGFTMAFHALELVRQGRTSLAEALRI
                     GFDVDDQDDQG"
     gene            1402..>2667
                     /locus_tag="PLB25_10375"
     CDS             1402..>2667
                     /locus_tag="PLB25_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HOE42018.1"
                     /translation="MAVYAWRGRNVRGEAVSGQLDALNENSVADQLMAMGVAPVHIGS
                     MPEKTQSVGDNWFERLSRAPVVVEDVLIFSRQMYTLNKAGVPILRAFAGLEASSTKPA
                     MVALLKDIRASLDQGRELSAALARHPGVFGAFYVSMIRVGEMTGRLTEVFLRLNEHME
                     FERDVRERIKQAMRYPMFVMIAMAVAVVILNIFVIPVFAKVFAGFNAELPLITRGLLG
                     FSAWMINWWPLLIAAVVGLGMLVRGYVRTPAGRYNWDARKLKLPIVGEIILKATLARF
                     ARSFALSIQSGVPLVQALTVVAQTVDNAFIGSRIEQMRDGIERGESISRCAAATGVFT
                     PVVLQMISVGEETGELDNLLFEIAGMYERETDYNIRGLSAAIEPILLAIIGALVLLLA
                     LGVFLPLWSMGQAAMGKGGEIFVFDMGEPV"
     assembly_gap    2668..2677
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2678..3213
                     /locus_tag="PLB25_10380"
     CDS             <2678..3213
                     /locus_tag="PLB25_10380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42019.1"
                     /translation="GHGQGRRLTSMQSLRQPLGLSNWRRAFEWALVAAVILVLAVVFV
                     RHMRAVQGQSEQAAVRTTLGALRTALVIEHLQHSLAANGGLSATSEPRSPFDLLQYRP
                     ANYIGIVNARRALVVPPGSWVYDPVCPCVGYRPLDDLWLNSPSGEPMAWYLISGAPGP
                     LQLTARENYLWQDQMLD"
     gene            3329..3709
                     /locus_tag="PLB25_10385"
     CDS             3329..3709
                     /locus_tag="PLB25_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOE42020.1"
                     /translation="MKRVQRGFTLIELVMVIVILGVLAAVALPKFVDLKGDAVAAATQ
                     GVAGALSSAAAINYAARNVSTSKGAAVANCTDVGSSTIMQGGLPSGYTITAGVVNAGA
                     SVTCTLTNAGPPSTTATFTAIGIP"
     gene            complement(3757..5955)
                     /locus_tag="PLB25_10390"
     CDS             complement(3757..5955)
                     /locus_tag="PLB25_10390"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HOE42021.1"
                     /translation="MLPPVNTLDLTGDPLRSMATAPKWHPQATNGPAAVLGLMMFLLP
                     TLGVPHELMLQDTLKSAIVAFGVLLAALLFFWQQRHRTEPMRWHGLVWLPLVLLLYAL
                     GSMVWSHSYLAGVEAIRWFVLSLLLWVGQNTLTRLNLPLLLWGVHWGAVLASVWAALQ
                     FWLDIRLFPQGASPASTFINRNFFAEYAVCALPFSVYLLATLGRPRWLIWQAMTVGFI
                     AVAIMMTGTRSALLALLLQCVVFAIIGFRYRQQLGIGQWSRSRQVQVGLVLMLTVLGL
                     GSIPSGNARILTEHSGSAALQRGYLRVSSMVNAKAYTEGSFSIRAVLWKASARMMLAH
                     PLTGVGAGAWEVQIPLYQGAAHSLEADYFAHNEFLQLLSEYGCVVGGLFLAVLFAYLL
                     QAASKTWSLAGADVEQAPLRGMVLGSLLTLLIVSSAGFAWHLAATGALFALCLAILAA
                     SDAQLAFPGSFFAATLRWHPNWSLAMLCGLAGCTLLAAFVTHQAACAERSLVQAIVLA
                     DSLRHPHADDASIIDRRTRALEAVRAGIAINPHYRKLTAEVAEPFAAGGDWANAVWIL
                     ASVAASRPHVYAIWSGLATGYAQLGRHDQARQAFVQVQRLKPDAISTLTLELILLASQ
                     GQTDKATELINSHFDQGRFDYDMVQSGYALGYQTGNWSLAIRALTLRMTTWPEHAPDA
                     FFRLGQLYSSPSLHDDAKALAAFRLGLAAVPAAQKDNYRSQVPAKFRSQM"
     gene            complement(6271..6346)
                     /locus_tag="PLB25_10395"
     tRNA            complement(6271..6346)
                     /locus_tag="PLB25_10395"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6311..6313),aa:Thr,seq:tgt)
BASE COUNT         1352 a         1873 c         2012 g         1343 t
ORIGIN      
        1 ggacctgttc gcctacgacg aactaacccg gctgctaaag cgcaatattt cagttgcggc
       61 ggtgcctgag agtcagttgg ctttggcctt tgaccgcttg tatcggcgca ccgaagaaat
      121 cagtggcctg gcccgggcac ttgagaaaga tctgggcgac gcagttgact ttggcgaact
      181 gacggccacc gtgggtaccg agggcgcgcc ggtggtgcgt ttgttgcagt cgctgtttga
      241 agatgcaatg caggttggtg cctcagacgt gcacatcgaa ccgcaagagg cgggcttgca
      301 gattcgggtg cgggtcgatg gcgtgctgca aactcagacg caggccgaca agcgcattgg
      361 cggtgcgctg gcgcagcgct tgaaattgat ggccgggctg gatatttcag aaaagcgcct
      421 gccgcaggac ggtcgtttta gcgtacgcct gcgcgacatc acgattgacg tgcgtttgtc
      481 taccttgccg accaactacg gcgagtcagc ggtgatgcgt ctgctcaagc agggctcggg
      541 catgaaacgc cttgacgaca taggcatgcc ggcagacatg ctcaagcggt tccgggaagt
      601 actaggacga tcctccgggc tggtgctggt gaccggccct accggttccg gcaaaaccac
      661 cacgctgtat gcggccctgg ctgaaatcaa cgctggtgaa ctcaaagtca tcacggtcga
      721 agacccggtc gaataccgct tgcctggcct cacccaggtg caggtcaacg acaaaattga
      781 actcactttt gcccgcgtct tgcgggcttg tttgcggcag gacccggacg tcattctggt
      841 cggcgaaatg cgtgaccctg agactgccga aatcggcttg cgggccgcga tcaccgggca
      901 tctcgtgcta tcaactttac acacccggga tgccatcagc acgccttttc gactgctcga
      961 catgggggtg ccaccgttca tggtggcaac ttcattgcag gcggtgattg cccagcgcct
     1021 ggtgcggctg aattgcacgg attgtgccga gccgcatgaa cccagcccgc aagagcagtc
     1081 ctggctggtc ggcatgttgc cagcaggcgg caccgtgagc gctaggcgcg gccggggctg
     1141 ttcatcctgc aatggtaccg gctactcggg gcgacaaggc atttatgaac tgctcgaaat
     1201 ggatgccgga ctgacacagg ctgcctcaag atcagacccg gcagcattca tgaagctcgc
     1261 ccgtgaccgc atgaaggggt tcaccatggc gtttcatgcg ctggagctgg ttcgccaggg
     1321 tcgcacatcg ttggccgagg cgctgcgcat cggttttgac gtggatgacc aggacgatca
     1381 gggttaatcg acgaggttca catggctgtc tatgcctggc gcggtcgcaa tgttcggggc
     1441 gaagccgtat ccgggcagct tgatgccctc aacgaaaaca gcgtcgcaga tcagttgatg
     1501 gcgatgggcg tggcaccggt gcatatcggc tcaatgccag agaaaacaca gtccgtcggc
     1561 gacaactggt ttgagcgcct tagccgcgcc ccggtggtgg tggaagatgt gctgatcttt
     1621 tcgcgtcaga tgtacacgct gaacaaggca ggggtaccaa ttttgcgagc ctttgccggg
     1681 ctcgaggcat ccagcaccaa acccgccatg gtggcattgc tcaaagacat tcgcgccagc
     1741 ctggaccagg ggcgggaact gtctgccgcg ctggcgcgtc atcccggcgt gtttggcgcg
     1801 ttttacgtgt caatgatccg tgtgggcgag atgaccgggc gtttgaccga agtatttctg
     1861 cggctcaacg agcacatgga atttgagcgc gacgtgcgtg agcgaatcaa gcaggccatg
     1921 cgttacccga tgttcgtgat gatcgccatg gcagtggcag tggtcatcct gaatattttt
     1981 gtgattccgg tgtttgccaa ggtgtttgcc ggctttaacg ccgaattgcc gcttatcacc
     2041 agaggtttgc tgggtttttc tgcctggatg atcaactggt ggccgttgct gattgccgcc
     2101 gttgtcgggc ttggcatgct ggtgcgaggt tacgtgcgca cccctgccgg tcgctacaac
     2161 tgggatgcgc gcaagctcaa gctgccaatc gtgggtgaaa tcattctcaa agccacgctg
     2221 gcgcgttttg cccgcagctt tgccctgtcg atccaaagtg gcgtgccgct ggtgcaggcg
     2281 ctcacggtgg tggcgcaaac tgtggataac gcctttattg gcagccgcat tgagcaaatg
     2341 cgcgacggca ttgagcgtgg cgagagcatt tcgcgctgcg ccgcggccac cggcgtgttc
     2401 accccggtgg tgctgcaaat gatctcggtc ggcgaagaaa ccggcgaact tgacaacctg
     2461 ttgttcgaga ttgccggtat gtatgagcga gaaaccgact acaacatcag gggcctgtcg
     2521 gcggcgattg agccgatttt gctggccatc atcggggcct tggtattact gctggcgctg
     2581 ggggtgtttt tgcccttgtg gagcatgggt caggcggcca tgggcaaggg tggcgagatc
     2641 ttcgtcttcg acatggggga acccgtgnnn nnnnnnngtg gccatgggca agggcggcgc
     2701 ttgaccagca tgcaaagcct tcggcaaccc ttgggattgt ccaactggcg aagggccttt
     2761 gagtgggcct tggttgcagc ggtgatcctg gtgctggcgg tggtttttgt gcggcacatg
     2821 cgggcggtgc aagggcaaag cgagcaggcg gcggtgcgca ccaccctggg agccctgcgc
     2881 acggcgctgg tcattgaaca tttgcagcac agtctggcgg caaacggcgg gctgtctgct
     2941 accagtgagc cacgcagtcc atttgacctg ttgcaatacc gtccggccaa ctacatcggc
     3001 attgtcaatg cacgtagggc gctggtcgtg ccacctggga gctgggttta cgacccggtt
     3061 tgcccctgcg ttggttaccg tccgctggac gacctttggc tcaacagccc cagcggcgag
     3121 ccgatggcct ggtatttgat cagcggcgcg cccggtccgt tgcaactcac ggcccgggaa
     3181 aactatcttt ggcaggatca aatgctggac tgatatatcg ggcacggcca ggagagtgcg
     3241 tactagaatc gagacgctgc tacttatttt gaagtgcagg atttatggtc tggctttttg
     3301 ttcgagtttt gttaccaagg tggacaacat gaaacgggtg caacgcggct ttacattgat
     3361 tgaactggtg atggtgattg tgattttggg tgtgcttgcg gcggtcgcct tgcccaagtt
     3421 tgtcgacttg aagggtgacg cggtggctgc tgcgacgcaa ggtgtggcgg gtgcactgtc
     3481 atcagccgca gccatcaact atgcggcaag aaatgtcagc accagtaaag gcgctgctgt
     3541 tgccaactgc actgatgtag gcagcagcac catcatgcaa ggcggtttac cgagcggata
     3601 caccatcact gccggggttg taaacgctgg tgcgtcagtc acttgcacgc tgaccaacgc
     3661 tgggccaccc agcaccactg cgactttcac tgcaattggc atcccctgac ctttacatcc
     3721 gtctgccaga aaagccgttc ctgatgaacg gtttttctac atctgcgagc ggaattttgc
     3781 cggcacctgg ctgcggtaat tgtctttttg tgccgccggt acagcggcca aacccagccg
     3841 gaaagcggca agcgcctttg catcgtcatg caaggatggg ctgctgtaga gttggcccag
     3901 tctgaagaat gcatcgggcg catgttctgg ccaggtggtc atgcgcagcg tcaaagcccg
     3961 gatggcaagc gaccagttgc cggtttgata gcccaaggca tagccagact gaaccatgtc
     4021 gtagtcaaac cggccctgat cgaagtggct gttgatcaat tcagtggcct tatcagtctg
     4081 accttggctg gcaagcaaaa tcagctccag ggtaagcgtg ctgatggcat caggtttgag
     4141 tcgctgcacc tgcacgaacg cctgtcttgc ctgatcgtgt cggccaagct gagcgtagcc
     4201 tgtcgccaga ccgctccaga tggcgtacac atgaggccgc gacgcagcga cagatgccag
     4261 aatccacacc gcattggccc agtcaccgcc agccgcaaag ggttctgcca cttcggcggt
     4321 gagtttgcgg taatgcgggt taatggcaat gcccgcgcgg acggcttcaa gtgcccgcgt
     4381 tctgcgatca atgattgagg catcgtcggc atgcgggtgg cgcagtgagt ctgccagcac
     4441 gatggcttgc accaggctgc gttcagcgca ggccgcctga tgggtcacaa aggccgccag
     4501 cagcgtgcag ccagccagtc cgcacagcat cgccaatgac caattgggat gccatcgcag
     4561 cgttgccgca aagaagctgc caggaaaggc caactgtgcg tcagatgctg ccaaaatcgc
     4621 caggcacaac gcaaaaagag cgccggttgc cgccagatgc caggcaaacc cggcactgct
     4681 gacgatcaac agcgtcagca ggctgccaag gaccatgccg cgcaaggggg cctgctccac
     4741 atccgcccct gccaaactcc aggtcttgct ggccgcctgc agcaagtagg cgaacaggac
     4801 ggccagaaac aaacctccca ccacacaacc gtattcgctg agtagttgca aaaactcgtt
     4861 atgggcaaaa tagtcggctt ccagggaatg ggccgcgccc tggtagagcg ggatttgcac
     4921 ctcccaggcc ccagcgccga caccggtcag tgggtgggcc agcatcatgc gggcgcttgc
     4981 cttccacagc actgcccgga tggagaatga tccttcggtg tacgccttgg cattcaccat
     5041 ggaagagacg cgcaggtagc cacgctgcaa ggccgcgctg cccgagtgtt ccgtcaagat
     5101 tctggcattg ccgctgggta ttgaacccag tcccagcact gtcaacatca gcaccagccc
     5161 gacttgcact tgcctggacc ggctccattg gccaatgccc aattgctggc ggtacctgaa
     5221 cccaatgatg gcgaaaacca cgcactgcag cagcagggcc agcaaggcag aacgggtgcc
     5281 ggtcatcatg atcgccacgg caatgaaccc aactgtcatg gcctgccaaa ttagccagcg
     5341 tggcctgccc aaggtggcca gcaggtaaac cgaaaaaggc agtgcgcaaa cggcgtattc
     5401 ggcaaaaaaa ttgcggttga tgaaggtcga tgccgggctt gccccctgcg gaaagagtcg
     5461 gatatccagc cagaactgca acgccgccca tactgaagcc agcaccgcac cccagtgaac
     5521 accccacaac agcagcggca ggtttagccg tgtcagggtg ttttgaccga cccaaagcag
     5581 caagctcaaa acgaaccacc gaatcgcctc gacaccggcc agatagctgt gtgaccagac
     5641 catgctgccc agcgcgtaaa gcagcagaac cagcggcagc cacaccagtc cgtgccagcg
     5701 catcggctcg gtgcgatggc gctgttgcca gaaaaacagc agcgccgcca gcagcacgcc
     5761 aaatgccaca atcgccgact tcaaggtgtc ttgcagcatc aactcgtggg gcacgcccaa
     5821 agtgggcagc aaaaacatca tcaggccaag caccgcagcg ggtccatttg ttgcctgcgg
     5881 atgccacttt ggggctgtcg ccatggatcg gagtgggtca cctgtcaggt ccagggtgtt
     5941 gacgggtgga agcatgctct gattcagttt ttggggcgtg ccggtcggca gtcagccatt
     6001 atgaaggaca gcccccaccc aaaccccagg gagccatgtc acaacgaggt gtgagctcaa
     6061 agcggggggt gggcagctca ccggttttcc aattgaaacg ggattcgtac ccccacgttg
     6121 ctcgaaaacc tccatacgac cccccatttg tgaccccgca ttggtttgtc catcaaaagc
     6181 ggcctgccta ccccgcggta ctgcaactag ctgatttatc aacgaaaaag cctcccagta
     6241 tcgctacttg gaggcctttg gactgtctga tggtgccgat taacggattc gaactgttga
     6301 cctaccgctt acaaggcggt tgctctacca actgagctaa atcggctgcg ttgccatttt
     6361 acatgactga caagtcggta cagacttttg agccttgtcc agcgcggtct gagcttgaac
     6421 ggctggcata tgtcaatcac gacaggctga tagccgcctg gattggcgtg accatctggt
     6481 gttttgcgtc gcagcgggct cacagcaaac agcagcctgt cccaaaatca gaggagttat
     6541 gcgtcaaagc tgcttcaggc acccagcacc cggttcttgc cggcccgctt
//
LOCUS       DAOSLL010000212         6570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10651_length_6570_cov_5.726324, whole genome shotgun sequence.
ACCESSION   DAOSLL010000212 DAOSLL010000000
VERSION     DAOSLL010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10651_length_6570_cov_5.726324"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="PLB25_10400"
     CDS             <1..390
                     /locus_tag="PLB25_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE42022.1"
                     /translation="THTGRFEEAMLAKALAQQHNDEAQMASDFTALKSLEQAVLWRML
                     ELGTRFRPYDAEALQFYNDKTGDKVSVAKAQKALESLRARQPSLIWKSARSEYAVADA
                     AMLRWYAERVSAGTWPPAGPQLDWLDE"
     gene            525..737
                     /locus_tag="PLB25_10405"
     CDS             525..737
                     /locus_tag="PLB25_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42023.1"
                     /translation="MRQDSGIDGDAQRSSLLTRLLLLKVLDGLEETSRLARDHCASPI
                     PESMRWRNWAADAKGMTGDVLLRDEL"
     gene            complement(907..1125)
                     /locus_tag="PLB25_10410"
     CDS             complement(907..1125)
                     /locus_tag="PLB25_10410"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42024.1"
                     /translation="MDRVIQQGTAQMLTPIFDPTFSQLSFGIRPGRNAHQAIRQVQAL
                     GRCHGIPFALWPAELGSEPHSGTHGCHV"
     gene            1105..1389
                     /locus_tag="PLB25_10415"
     CDS             1105..1389
                     /locus_tag="PLB25_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42025.1"
                     /translation="MLYHPVHDSGYAQHALAAAGLWHLYSCARPAAGIARPGIAALAR
                     SLGDCDTNLLGMVGEVIVEGLLGLTKAPKQSVVCYLSWQIATPKNDQKSF"
     gene            1643..2794
                     /locus_tag="PLB25_10420"
     CDS             1643..2794
                     /locus_tag="PLB25_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE42026.1"
                     /translation="MLISNIRLKNWRNFQALDLPLRDVSYVLGANASGKSNLLDALRF
                     LRDVSKTKGGGLQAAIAERGGISKVRCLHARRDPEVEIDIELSQDLDQPFVWRYVLAF
                     KPEGKGAQRLLVSREQVWKDGTLLLNRPDLKDDADVLLRTQTHLEQIQTNIKFREIAD
                     FLNDITYLHLVPQLLRFGEKIGGHRLTDDPFGQGFLERLSKTPEKTRNSRLAKISKAL
                     SLAVPQFEDLRFSKDDMGHPHLEARYAHHRPNAGWQSEEHFSDGTLRLFGLLWALLEG
                     SSMLLLEEPEISLNDAIVKEIPLIVDRLQRDRKTKRQVILTTHSDALLSNPGIDGRGV
                     VILETGKQGTTGRPLDNSERQALGAGLSVAEVVLPKTRPAMVSQLGLWQ"
     gene            2791..3417
                     /locus_tag="PLB25_10425"
     CDS             2791..3417
                     /locus_tag="PLB25_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42027.1"
                     /translation="MTPIAIATEDQLSEAIALRLISDIPTPHQVTFKLGQRGNGYLRS
                     KMASWYQMAQHQVMLVLTDLDRANCLVEFRDQWLVTESPPNLLLRIAVREIESWVLAD
                     HVALRVLLGANGVLPAKPDELVDPKQSLLRLAKGAPRPIRLDLLRTTDGNLAQGLGYN
                     ARLSTWVNNEWSPQRAAERSPSLARARVRLGEAVGAFALSVHGQQCSR"
     gene            3655..5292
                     /locus_tag="PLB25_10430"
     CDS             3655..5292
                     /locus_tag="PLB25_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HOE42028.1"
                     /translation="MTARWRPDGWRLLSWAVAALVLVPVLVSFSSFTQLDRTTLAHLG
                     EFVLPELLVNTFWLLLGVGLGVSVLGVSLAWLVAMCEFPGRRVLDWALLLPLALPAYV
                     TAFVAIDLLDFSGPLQSWLRNGWGITGLPEVRSRAGVIAVMSLALYPYVYLIAKNAFA
                     TQGAMALEAAQSLGLSRPRGFFKVALPMARPWIAAGVMLALMETLADFGTVAVFNYDT
                     FTTAIYKAWYSLFSLPAAAQLASVLIMFVLIAVVIEQRSRAQMRFGAVGRASASRRIT
                     LTRQQGWLAFGYAGAVLLLAFVIPVGRLLLWTWHTAARDLDARYWSFTLHSLMLSALG
                     AMLVVAVGLLLAYAGRQRAGTGMVWAQRIATLGYAFPGAVLAVGLFIPVAALDNLLMD
                     AGRAWFGFAGTEVLKGTLLVMLLAYLVRFLAVGFGPIDSGLQRITRNVDEAARSLGCS
                     SAHLLGRVHLPMLRLSLQTAAALTFVDIMKEMPITLMTRPFGWDTLAVRVFEMTSEGE
                     WERAALPSLAIVLTGIIPILLLTWRNGAAHHEGPKHG"
     gene            5285..6352
                     /locus_tag="PLB25_10435"
     CDS             5285..6352
                     /locus_tag="PLB25_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE42029.1"
                     /translation="MDELVLDAVSQSYGTRQIISSLSFHLARGEIGCLLGPSGCGKTT
                     ALRCIAGFEPVQSGSICIAAATVSRPGLTLAAEKRRIGMVFQDYALFPHLTVAGNVGF
                     GLPQRNQPAAKARVDELLGLVGLAQHALKYPHQLSGGQQQRVALARALAPRPRLILMD
                     EPFSNLDVELRERLSIEVRDILKREATTALIVTHDQHEAFALADRVGVMHQGRIQQWD
                     TPYNLYHRPANRFVADFVGQGVMLSGDVLNSRQVRTELGVLDGQIPVDCSSGGCQACP
                     RSCGVDVLLRPDDIVHDDDSPLQAEVENKAFRGAEFLYTLKLPSGQRALSLVPSHHNH
                     AIGEKIGIRLSADHVIAFRRD"
BASE COUNT         1206 a         1931 c         2057 g         1376 t
ORIGIN      
        1 acccacaccg gccggtttga agaggccatg ctggccaaag cgctggcgca acagcacaac
       61 gacgaggccc agatggccag tgatttcacc gccttgaagt ctctggaaca agcggtgttg
      121 tggcgcatgc tggagctggg cacccgcttt cggccctacg atgccgaggc gctgcagttc
      181 tacaacgaca aaaccggcga caaggtgagc gtggccaaag cccaaaaagc gctggagagc
      241 ctgcgcgctc gccagcccag cctgatctgg aaatctgccc gcagcgaata cgccgtggcg
      301 gacgcggcca tgctgcgctg gtatgccgag cgcgtcagtg caggcacctg gccaccagca
      361 gggccgcagc tcgattggct ggacgagtga gccttggctg ccacgaaacg ctacgtatgt
      421 tgtagcttgt agcgcacgtt ctatatgggc tagagcccaa tttctcatgt aagccaaacc
      481 ccaggtccaa gctctcaagc gtcatcaaat ccatccagta cctgatgcgg caagacagcg
      541 gcattgacgg cgacgcgcag cgctcttcgc tgctcaccag gctcttgtta ctgaaagtgc
      601 ttgacgggct cgaagaaacg tcccgactcg cccgcgacca ttgcgccagc ccgattcccg
      661 aatcgatgcg ctggcgcaac tgggccgcag atgccaaggg tatgaccggc gacgtgctgc
      721 tgcgcgatga gttgtaggac atattgccaa atcgctgtct agcgcttgat ggcattgcgc
      781 taccagctat caaaatcatt gctatcactt gaaggaaggt aaatcgcacg ccgcattttg
      841 acccggtgcc aagcggcccc gcgcagtagc agatttgggg tcggatcccc attcggccca
      901 acttgctcaa acgtggcacc cgtgcgtccc cgaatggggc tctgacccca gttctgcggg
      961 ccacagagca aagggtattc catggcaacg ccccagcgcc tgcacctgcc tgattgcctg
     1021 gtgtgcattg cgacctggtc ggataccgaa actcaactgc gagaacgtcg ggtcgaatat
     1081 cggcgtgagc atttgggctg tgccttgctg tatcacccgg tccatgacag tggctatgcc
     1141 cagcatgcgc ttgccgccgc cgggctttgg catttgtatt cctgcgcacg gcctgcggct
     1201 ggtatcgccc gtcccggaat tgcggcactc gcccggtcgc tcggcgactg cgacacgaac
     1261 ctcctgggaa tggtcggtga agtcattgtc gaaggcctac ttgggctgac gaaggcacca
     1321 aagcagagcg tggtttgtta tttgtcttgg cagattgcaa cgccaaaaaa tgaccaaaaa
     1381 tcgttttaaa tcaataacct ggataacgag ttgttttcca tttgccaaaa acaaatgact
     1441 tcggtgatcc cgagttgcct gtgggcaagc gttgggcaga acattgtgtc agtattgccg
     1501 agtcgcatcc ctgggtcacc tgtgctctga tttgtactag cgcctggtca ccagcaaagc
     1561 cagccaagcc agccaagccc atctggccga ggcgctgata gaaaatgccg gttagtcccc
     1621 ttcacccgcc caaggccaca ccatgctcat cagcaacatc cgattaaaaa actggcgcaa
     1681 cttccaggcg ctggatttgc ccctgcgcga tgtgtcctat gtgctggggg ccaatgcgtc
     1741 tggcaagtcc aatttattgg atgcgttgcg atttctgcgt gatgtcagca aaaccaaggg
     1801 tggcggcttg caggcggcca ttgcagagcg gggcggcatc tcaaaggtgc gctgtcttca
     1861 tgcccggcgt gaccccgagg tggaaattga cattgaactc agtcaggatc tggatcaacc
     1921 ttttgtctgg cgttacgtgc tggctttcaa gccagagggc aagggcgccc agcgcttgct
     1981 ggtttcgcgc gaacaggttt ggaaagacgg cacgctgttg ttgaatcgac cagacctcaa
     2041 ggacgatgcc gacgtgttgc tgcgcaccca aacccatctg gagcaaattc agaccaacat
     2101 caagtttcgc gaaatcgctg attttttgaa tgacatcaca tacttgcatc tggtgccgca
     2161 gttgttgcgc ttcggcgaaa agattggcgg acatcggctc acggatgatc cttttggcca
     2221 aggctttttg gaacgtttgt ccaagacacc agagaaaacc agaaactcgc gtctggccaa
     2281 gatttcaaaa gccttgtctt tggcggtgcc gcaattcgag gatttgcgtt ttagcaagga
     2341 cgacatgggc cacccgcacc tggaggctcg ctatgcccac catcgcccca atgcagggtg
     2401 gcagtctgag gaacattttt ccgacgggac gctgcgcttg tttggtctgt tgtgggcgtt
     2461 gctggaaggc agctccatgt tgttgctgga ggagccggaa atttcgctca acgatgccat
     2521 cgtgaaagaa attccgctca tcgttgaccg cttgcagcgt gaccgcaaga ccaaacggca
     2581 ggtgattttg acgacgcaca gcgatgcact tctgagcaat cccggcattg atgggcgggg
     2641 tgtggtgatt ctggaaaccg gcaagcaagg cactaccggg cggccgttgg acaacagcga
     2701 gcgccaagcc cttggagccg ggttgagtgt tgccgaagtc gtgttgccca agacgcggcc
     2761 agccatggtg tcgcagttgg gtttgtggca atgacaccga ttgcgattgc aacggaagat
     2821 caactcagcg aggcgattgc actgcgcctg atttctgaca ttccaacacc gcaccaagtc
     2881 actttcaaat tgggccaaag gggcaacggc tacttgcgct ccaaaatggc cagttggtac
     2941 caaatggcgc aacaccaggt gatgctggtg ttgaccgacc tggatcgcgc gaattgtctg
     3001 gtcgagtttc gtgatcaatg gttggtcacc gagtcgccac cgaatctgct gctcagaatt
     3061 gcagttcgtg aaatcgagtc ttgggtgctg gcagatcatg ttgcgctgcg ggtgctgctt
     3121 ggtgccaatg gggtcttgcc tgccaagcct gatgaactgg tggaccccaa gcagtctttg
     3181 ttgcgactcg cgaaaggcgc gccgcgacca atcaggctgg acttgttgag aaccactgac
     3241 ggaaatttgg cgcaaggttt gggctacaac gcccgcctgt ccacatgggt caacaacgaa
     3301 tggagtccgc agcgcgccgc agaacgctcg cccagcttgg ctcgggcacg agtgcgcttg
     3361 ggggaggctg tcggcgcgtt cgctttgtct gtgcatggtc agcaatgttc ccgatagcgt
     3421 ttcacaaaaa agggtcgtgt tggctcaact ctgcgccacg agccgctgaa agttgacacg
     3481 ggtcatgccg gccagaatgt tgcctgtaac gcaaggtgtg gcttatccag gagcgcaaaa
     3541 gcttacccgg cagcgatact tgacccccat gaattcacgc cccagcccat cagccacccc
     3601 cagccacagc ggctggcgcg gctggcgcgg cccccctgag cctggggcag cagcgtgacg
     3661 gcgcgctggc ggccagacgg ctggcggctg ctgtcctggg cggtggccgc cctggtgctg
     3721 gtgccggtgc tggtcagctt ctcgtcgttc acccagcttg accgcaccac gttggcccac
     3781 ctgggcgaat ttgtcttgcc agaactgctc gtcaacacct tctggctttt gctcggggtc
     3841 gggctggggg tcagcgtgct gggggtgtcg ctggcctggc tggtggcgat gtgtgagttt
     3901 cctggccgcc gggtgctcga ctgggcgctg ctgctgccgc tggccttgcc agcctatgtc
     3961 accgcgtttg tggcgattga cctgctcgac ttcagcgggc cgctgcaaag ctggctgcgc
     4021 aacggctggg gcattaccgg cctgcccgag gtgcgctcac gcgctggcgt gatcgccgtg
     4081 atgtcgctgg cgctgtaccc gtatgtctat ctgattgcca aaaacgcctt tgccacgcag
     4141 ggcgcgatgg cgctggaggc agcgcagtcg ctgggtttgt cacgaccacg cggttttttc
     4201 aaggtggcgc tgccgatggc gcggccgtgg attgccgccg gggtgatgct ggcgctgatg
     4261 gaaaccctgg ccgactttgg caccgtggcg gtgttcaact acgacacctt caccaccgcc
     4321 atttacaaag cctggtacag cctgttttct ctgcctgcgg cggcgcaact ggcctcggtg
     4381 ctgatcatgt ttgtgctgat tgcggtggtg atcgaacagc gctcgcgcgc gcaaatgcgc
     4441 tttggcgcgg tcgggcgcgc ttcagcctcg cggcgcatta ccctcacgcg ccagcagggc
     4501 tggctggctt ttggctacgc gggggcggtg ctgctgctgg cgtttgtcat cccggttggg
     4561 cgactgctgc tgtggacctg gcacacggca gctcgcgatc tggatgcgcg ttattggtct
     4621 tttacccttc actcccttat gctgtctgcg ctgggagcta tgcttgttgt agcggttggg
     4681 ttgttgctgg cgtatgccgg acggcagcgg gctggcaccg gcatggtctg ggcgcaacgc
     4741 atcgccacgc tcggctacgc gtttccgggg gcggtgctgg cggtcggcct gttcataccg
     4801 gtggcagcac ttgacaacct gctgatggat gccgggcgtg cctggttcgg ctttgccggc
     4861 accgaggttc tcaagggcac gctgctggtg atgctgttgg cctacctggt gcgctttctg
     4921 gccgttggct ttggcccgat tgacagcggc ctgcagcgca tcacccgcaa tgtggacgag
     4981 gccgcacgca gcctgggctg cagcagcgcg cacctgctcg ggcgggtgca tttgccgatg
     5041 ctgcgcctga gcctgcaaac cgccgctgca ctgacctttg tggacatcat gaaagaaatg
     5101 ccgatcaccc tgatgacgcg cccgtttggc tgggacacgc tggcggtgcg ggtgttcgag
     5161 atgacctccg agggcgagtg ggaacgcgcc gcgctgccat cgctggccat cgtgctgacc
     5221 ggtatcatcc cgatcctttt gctgacctgg cgcaacggtg cggcgcacca cgaaggaccc
     5281 aagcatggat gaactggtac tggacgcggt ttcgcagtcg tatggcacgc ggcagatcat
     5341 cagcagcctg agctttcatt tggcccgggg cgaaattggc tgcctgctgg ggccatcggg
     5401 ctgcggcaaa accaccgcgc tgcgctgtat tgccggtttt gagccggtgc aaagcggctc
     5461 aatctgcatc gctgcagcga cggtcagccg ccctggcctg acgctggctg cggaaaagcg
     5521 ccgcattggc atggtgtttc aggattacgc gctgtttcct cacctgacgg ttgctggcaa
     5581 tgtcggtttt ggcctgccac agcgcaacca gccagcggcc aaggcgcggg tggacgaact
     5641 gctcgggctg gtcggcctgg cccagcacgc gctcaaatac ccgcaccagc tctcgggcgg
     5701 ccagcagcag cgggtggcac tggcacgggc gctggcaccg cggccccggc tgatcctgat
     5761 ggatgagccg ttttccaacc tggacgtgga actgcgcgaa cgcctgtcga tcgaggtgcg
     5821 cgacattctg aagcgcgaag ccaccacggc gttgatcgtc acgcacgacc agcacgaggc
     5881 gtttgcgctg gccgaccggg ttggcgtgat gcaccagggc cgcatccagc agtgggacac
     5941 gccttacaac ctgtaccacc gcccggccaa tcgctttgtg gcggactttg tcggccaggg
     6001 tgtcatgctg tccggcgatg tgctcaacag tcggcaggtg cgcaccgaac tgggcgtgct
     6061 cgatggccag attccagtgg actgcagcag cggcggttgt caggcttgcc cgcgcagttg
     6121 cggtgtcgac gtgctgctgc gcccggacga catcgtgcat gacgacgaca gcccgctgca
     6181 ggccgaggtg gagaacaagg catttcgcgg tgccgagttc ctctacaccc tgaagctgcc
     6241 cagtggccag cgggcgctct cgctggtgcc ctcgcaccac aaccacgcca ttggtgaaaa
     6301 aattggcatc aggctgtcag cggaccatgt gatcgcgttt cggcgcgact gacaacgcgg
     6361 tggcttgatc aaagcccggc aagaagggcg tacccggcag cgcccttgcg ccaaaccatt
     6421 tccgcccgga tcagcaaggc tcaacgtcca gtgcagccgg ttggcctgtc ggagcggcaa
     6481 tgaatggcat gatgctgcct ttggctttta atctctaaca gctggacagt gccaagttct
     6541 ccccgttatt caggcggttt cttggccagg
//
LOCUS       DAOSLL010000213         6568 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10662_length_6568_cov_3.966528, whole genome shotgun sequence.
ACCESSION   DAOSLL010000213 DAOSLL010000000
VERSION     DAOSLL010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6568)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6568)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6568
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10662_length_6568_cov_3.966528"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1069)
                     /locus_tag="PLB25_10440"
     CDS             complement(143..1069)
                     /locus_tag="PLB25_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013090858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42030.1"
                     /translation="MSSTITKRKGRGRSVKSMALIQAAIRILQEIQPCSIRAVCYRLF
                     TEKLIPDMGKKSTGAVGTQLVYARESGLIDWSWIVDETREAETVKSWSDPESIIMAAV
                     QQYRKDYWETQPRRVEVWSEKGTIRGTVAPILKKYGVTFRVLHGYGSATSIHGIAQET
                     QASDKLMTVFYIGDHDPSGRHMSEVDLPERLARYGGSALIIRLALADSDVTADSKLPW
                     FPVGDKINDTRYQWFTERFGQRCWEVDALPPPVLRARLDSAIANELDLDAWQHAVKIE
                     KAETDSMKNIMGYFKASISRLDPKYSDRGAHG"
     gene            complement(1062..1829)
                     /locus_tag="PLB25_10445"
     CDS             complement(1062..1829)
                     /locus_tag="PLB25_10445"
                     /inference="COORDINATES: protein motif:HMM:NF021206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HOE42031.1"
                     /translation="MHTNIKQKAPRACDSKGLHINTNASYFASHGSIQQDHGGNNTSL
                     AIAPDDFALITTATEARVDTRLLARQLGNKHKPVIALIDKYQKELERFAKVLFKKAPS
                     GGKTGQLERFALLNEDQAFFVLALSRNTDTVVTLKSKLVTAFGNARRAAAMRQTEYLP
                     EYHHLHDRIGELAAGSPNERWTHANVNKAVNKFAGIVPGQRASAGLPQQALLIVAQLV
                     AAKAAQGATDHHDGYQRIKSSLKALQGTLALEVAGHE"
     gene            complement(1840..2142)
                     /locus_tag="PLB25_10450"
     CDS             complement(1840..2142)
                     /locus_tag="PLB25_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HOE42032.1"
                     /translation="MSPTITRFKTKPTAPTPAASTAPTQFVFDALPDSAWLRESQLVR
                     SPKNPDSAIAPLPFSAPTLWRMVKAGRFPKPHKLSERVTAWQVAQVREWMTAQAAA"
     gene            complement(2259..2690)
                     /locus_tag="PLB25_10455"
     CDS             complement(2259..2690)
                     /locus_tag="PLB25_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42033.1"
                     /translation="MSIKIPDGYVLCKKGGRPKKDGRHIAVLMAQLLRIEIHKEKSYM
                     AHEWILSHWQDRSIDDESAIRREIRLARPLMDKHLIIISENYIHAIRTPLVEGSLAWF
                     WHVGIHEALRLKVENLTGVVQFEAPQFIPLAMLAKRTPTQR"
     gene            complement(2786..4006)
                     /locus_tag="PLB25_10460"
     CDS             complement(2786..4006)
                     /locus_tag="PLB25_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HOE42034.1"
                     /translation="MALTDTFVKQVKHSGKAAGDRYTDGSNMYLHVKAAGKYWRMDYT
                     HSAKRKTLALGVYPAVSLLKARQRRDKARELLADGIDPGAAKQEAKQAQAAAMANTFE
                     AVARDWMEKTAADRIASTTQKIDNWMTHDVYPYIGTKPISTIGPRDVLAALRHMEARG
                     ALESVQRVKQHIGQVFRYAVATGSAERDPTPDLKGALTKHAPKNYAAITDPNQAGELM
                     RSIFNYAGHPNVIAALKLSPLVFVRPGELRGAEWAEIDLDAAQWVIPASKMKMKVDHI
                     VPLCTQAVEILKGMKLLTGHRRYVFPSIGRSEDRPMSENTINSALHAMGYGTDKHTAH
                     GFRAMARTIMDEVLEERVDLIEHQLAHQVKDPNGRAYNRTAHLPARRAMMQRWADYLD
                     KLRIGADVIQFKAA"
     gene            complement(4564..5175)
                     /locus_tag="PLB25_10465"
     CDS             complement(4564..5175)
                     /locus_tag="PLB25_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HOE42035.1"
                     /translation="MNKRNAIKTIAISAIWTGAAGLFVACSEGAPKFTAIDITGADYA
                     KDFALVDHNGQARTLKDFHGKVVMMFFGYTQCPDVCPTSMTEMVAIKKLLGKDGNRLQ
                     GLFVTIDPGRDKADMLKAYMENFDPTFLALLPAPDQLAALAKDYKVYYRKVDGPTPTS
                     YTMDHTAGSYVYDPQGKLRLFTRYGTKPELTAADIQMLLKLSG"
     gene            complement(5243..6151)
                     /gene="cyoE"
                     /locus_tag="PLB25_10470"
     CDS             complement(5243..6151)
                     /gene="cyoE"
                     /locus_tag="PLB25_10470"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HOE42036.1"
                     /translation="MSAPTGPATATTLSVWAQFYALTKPRVIQLIVFCALIGMVLAVP
                     GVPSWPQAGKALLACIGIWLVAGAAAAFNCIIEKSIDAKMKRTAWRPTAKGQLGDRQT
                     LLFSASLCALGSGLLYLAVNPLTMWLTFATFIGYAVIYTLILKPLTPQNIVIGGASGA
                     MPPVLGWAAMAGNVGPEALILFLIIFLWTPPHFWALALYRVEDYRKSGLPMLPVTHGN
                     EFTRLMVLLYTFILLAACLLPFVYGMSSWLYLVVAVALSGGFCLHGWWLWRDYSDVLA
                     RKTFRFSLIHLSALFAALLIDHYLPG"
     gene            complement(6148..>6568)
                     /locus_tag="PLB25_10475"
     CDS             complement(6148..>6568)
                     /locus_tag="PLB25_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HOE42037.1"
                     /translation="PTCQGSWWPDMNLAQGFEIWRDLGLTGSGEPISFAALTAIHYMH
                     RLLAYGVLGLLMLLAWQLNRSNAWRLQSRLLAALIGLQLATGLANVVLGWPLLAALLH
                     TGGAAALVLTLTWVGAGAAPRRKVLPAGGPTVSGRQP"
BASE COUNT         1343 a         1884 c         1825 g         1516 t
ORIGIN      
        1 gtttcggaca tactgcctcg ccacctcgat cagcagcgcc gccgccgggt agctgagcga
       61 ttgatacgcg tgcgagtcga tcacgcacca gggcaatgcg cagtacccgc ccggttcccg
      121 gctttcgtca acacggggtt ttttagccat gcgccccccg atcagagtat tttgggtcta
      181 gcctggaaat actcgccttg aagtagccca tgatgttttt catggaatcg gtttcggcct
      241 tttcgatctt cactgcgtgc tgccatgcgt ccaaatccag ctcgtttgca atggcgctgt
      301 cgagccgcgc gcgcagtacc ggcgggggca atgcgtcaac ttcccagcag cgctgcccga
      361 agcgttcggt aaaccactga tagcgggtgt cgttgatctt gtcgcccacg ggaaaccagg
      421 gcaacttgct gtcagccgtc acgtcactgt ctgcaagtgc cagccgaata atgagggcac
      481 tgccgccgta ccgtgcgagt cgttcaggta gatcaacttc ggacatatgc cgcccggatg
      541 ggtcgtggtc gccgatgtaa aacaccgtca tgagcttgtc gctggcctgc gtttcctggg
      601 caattccgtg gatagaagtc gcgctcccat agccatgcag cacgcgaaaa gtgacaccgt
      661 actttttcag aatgggtgcc actgtgccgc gaatggtgcc tttctcgctc catacctcta
      721 cccggcgtgg ctgggtttcc cagtagtctt tgcggtattg ctgtaccgcc gccatgatga
      781 tggattcagg gtctgaccat gatttgacgg tttcagcttc gcgggtttcg tcaacaatcc
      841 aagaccagtc aatcagccca ctttcacggg cataaacaag ctgggtgccg actgcgcccg
      901 tgctcttttt gcccatatct gggatcagct tttcggtgaa cagccgatag cagactgccc
      961 ggattgaaca tggctgtatc tcttgcaaga tgcggatagc ggcctgaatc agcgccatgc
     1021 tcttgactga gcggccccga cctttgcgtt tggtgatggt gctactcatg tccagccacc
     1081 tccagcgcca gcgtgccctg tagcgccttc aacgatgatt tgatacgctg atacccgtca
     1141 tgatgatctg tcgcgccctg agccgctttt gctgccacca gttgcgcaac aatcagcagc
     1201 gcttgctggg gtagtccggc gctggcacgc tggccaggca cgatgcctgc aaacttgttc
     1261 accgccttgt tgacgttcgc gtgagtccag cgctcattgg gggaacccgc cgccagttca
     1321 ccgatgcggt catgcaggtg gtggtattcg ggcaaatact cggtttggcg catcgctgcc
     1381 gcccggcgtg cgttgccgaa tgccgtcacc agcttggatt tgagcgtcac cacggtgtcg
     1441 gtgttgcgtg acaaggccaa aacaaaaaac gcttgatctt cattcagcag cgcaaaacgt
     1501 tcaagctggc ccgtctttcc gcccgaaggt gcttttttaa aaagcacctt agcgaagcgt
     1561 tcaagctctt tctggtactt gtcgatcagt gcaatgacgg gtttgtgttt gttgccaagt
     1621 tgccgcgcca gtaaccgtgt gtccactctc gcttcggtcg ccgtggtgat cagagcgaag
     1681 tcatccggcg caatggccag gctggtattg ttgccgccgt ggtcttgttg aatcgacccg
     1741 tggctggcaa aatacgacgc gttggtgttg atatgcaagc ccttggagtc gcaagctctg
     1801 ggggcttttt gcttgatgtt ggtgtgcatg gccagcccct tatgccgctg cctgtgccgt
     1861 catccattcg cgcacctgtg ccacctgcca ggctgtcacg cgctcactaa gtttgtgggg
     1921 tttgggaaac cgtccagcct tcaccatgcg ccacagcgtc ggtgctgaaa atggcagcgg
     1981 ggcaatggcg ctgtccgggt tctttggact gcgcaccagt tgagattccc gaagccatgc
     2041 gctgtcgggc agtgcgtcaa agacaaactg agtcggtgct gtagatgccg ctggcgtggg
     2101 tgccgtgggc ttggttttga accgggtgat ggtgggtgac atatcgggat tccttatgaa
     2161 agaaacccga gtctcaaaac agtggtcgtt tttggtaagg tcaaatatgg ttattgaccg
     2221 gggcataaac agtttttgcc cttacaaatt tgatagcact aacgttgcgt tggggtgcgc
     2281 tttgcaagca tggctagagg gatgaattgg ggtgcctcaa actgcacgac acctgtcaaa
     2341 ttttcaactt ttaaccgtaa cgcctcgtga attccaacgt gccaaaacca ggctagtgac
     2401 ccttctacca acggtgtcct gatggcgtga atataatttt ctgaaatgat aataaggtgc
     2461 ttatccataa gcggccgggc taatcgtatt tcgcgcctta ttgccgactc gtcatcaata
     2521 ctccggtctt gccagtggct caaaatccat tcatgcgcca tgtaggattt ttctttgtgt
     2581 atttctatac gcaacaactg agccattaaa acggcaatgt ggcgaccatc ttttttgggt
     2641 ctaccgccct ttttgcacag cacatagcca tctggaattt tgatactcat accgcccctc
     2701 ttcacgccct gagaatgaac cagcgccagg cagatcaggg gtaatctgct tttcgccccg
     2761 tcgggctagg cgtggtcaaa aactgttatg ccgccttgaa ctggatcacg tccgcgccga
     2821 tgcgcaactt gtccaaatag tccgcccagc gctgcatcat ggccctgcgt gctggcaggt
     2881 gcgctgtccg gttgtaggcc ctgccgttgg ggtctttgac ttggtgcgct aactggtgct
     2941 cgatcaggtc aacccgttct tccagcacct catccatgat ggtgcgtgcc atcgcccgaa
     3001 aaccatgggc agtatgtttg tcagtaccgt agcccatggc gtgcaatgcg ctgttgatgg
     3061 tgttctcact cattggccga tcctcgctgc gcccaatact ggggaacaca tagcgtctgt
     3121 gcccagtcag cagcttcatg cctttcagaa tctctactgc ctgagtgcaa agcgggacga
     3181 tgtggtcaac cttcattttc atcttgctgg caggaatcac ccattgcgcc gcgtccagat
     3241 caatctctgc ccattctgcg ccgcgaagtt caccaggccg gacaaacacc agcggtgaca
     3301 atttcagcgc tgcgatgacg ttggggtgtc ctgcgtagtt gaagatgctg cgcatcagct
     3361 caccagcctg attagggtcg gtaatggcgg cgtagttctt tggggcatgt ttggtcagtg
     3421 cgcctttcag gtctggcgtt ggatcgcgct ctgcgctgcc agttgcaacg gcataccgaa
     3481 acacctgccc aatgtgttgc ttgacgcgct gcacgctttc caatgcgcca cgtgcttcca
     3541 tgtgccgcaa agcggccaac acgtctcgcg gcccaatggt cgatatgggc ttggtcccga
     3601 tgtatgggta cacgtcatgg gtcatccagt tatcaatctt ttgggtggtg ctggcaattc
     3661 gatcagccgc agtcttttcc atccagtcgc gtgctaccgc ctcaaaggtg ttggccatcg
     3721 ctgccgcctg agcttgtttg gcttcctgct ttgctgcgcc agggtcgatt ccgtcagcga
     3781 gtagctctcg tgctttgtcg cgccgctggc gtgccttgag tagcgacacc gccgggtaaa
     3841 cgcccaaggc tagcgttttg cgcttggcgc tatgggtgta gtccatgcgc caatacttac
     3901 cagcagcttt gacgtgcaag tacatgttgc tgccgtccgt gtatctgtcg ccagcagctt
     3961 tgccgctgtg cttgacttgc ttcacgaaag tgtctgtcag tgccatgttt gccccttcgg
     4021 tcgatcaaat acagtatctg ctttccagta cttgccagat atggttaaaa gtactgtaaa
     4081 aggtttgcgc tgtaatgata cgacttgaca cccaataagg caaagaaaaa cccgctacgc
     4141 tagggaaacg tgcgggtttt gatagggttt gatacggtat gaaaccgtca aatggtgccc
     4201 cgagccggaa tcgaaccggc acgccatttc tggccacgga ttttcttacc acttcgattt
     4261 tcaccgccac attgcatgcg ttcgtggtct ggagtacgcc ttgaccatga caccattact
     4321 ggtgctgtag gcccccgccg tctactctct acaccttccc ccgatcaggg ggctcggctc
     4381 ggcgttgcca tgcgcacaaa actgtgcgcg aagggttcac cgaatttgac gggattcaca
     4441 ctgagcgttt ccacacaggt gctcaaattg ttcaagtccg ttacgtctac ctatttcgtc
     4501 atcagggcac gccagtttga attatcgcct aagtcaaaat tcactcaacc aggcgggcgc
     4561 aattcaacca gagagcttca gcagcatttg aatatccgct gcggtcagtt ccggcttggt
     4621 gccgtagcgg gtaaacaggc gcaacttgcc ctgggggtca tacacgtagc tgccagccgt
     4681 gtggtccatg gtgtagctgg tcggcgtggg gccatcgact ttcctgtaat agaccttgta
     4741 gtctttggca agtgcggcca gttgatcggg tgccggcaat agcgccagaa aggttgggtc
     4801 gaagttctcc atataagcct tgagcatgtc ggccttgtcg cgcccagggt cgattgtgac
     4861 aaacaggcct tgcaggcggt taccgtcctt gcctagcagc ttcttgatgg ccaccatttc
     4921 ggtcatcgag gttggacaga catccgggca ctgggtgtag ccaaagaaca tcatgacgac
     4981 cttgccatgg aagtctttga gggttctggc ctggccattg tggtccacga gggcaaagtc
     5041 cttggcatag tcagccccgg tgatgtcaat cgcggtaaac ttcggggcac cctcggaaca
     5101 tgcaacaaaa aggcctgcag cgcccgtcca tattgcgctg atagctattg tttttatagc
     5161 gtttcgtttg ttcatgggtt tgatcataaa ccgctggcag tttggcccgc agcgaagcgc
     5221 cacggctcac actgctgccc gctcagcccg gcaggtagtg atcgatcaac agggcagcaa
     5281 acagcgcgct caaatgaatc agcgaaaacc ggaaggtctt gcgcgccagc acatcagagt
     5341 aatcgcgcca cagccaccat ccatgcagac agaagccgcc actcaaagcc acagccacca
     5401 ccaggtaaag ccaggaactc atgccataga caaatggcaa cagacatgcg gccagcaaaa
     5461 tgaaggtata gagcagcacc atcagccggg taaattcatt gccatgcgtc accggcaaca
     5521 ttggcaaacc ggacttgcgg tagtcttcca cccggtacag tgccagcgcc cagaagtgcg
     5581 gcggtgtcca caagaaaatg atcaagaaca ggatcaaggc ctccggtccg acattgcccg
     5641 ccatggccgc ccagccaagt accggtggca tggcaccgga ggcaccgcca atcacaatgt
     5701 tttgcggcgt caggggcttc aagatcagcg tatagatcac cgcatagccg ataaaagtgg
     5761 cgaaggtcag ccacatggtc agcggattga cggcaaggta gagcagccct gagcccagtg
     5821 cgcacaagct ggcagagaac aacagggttt ggcggtcacc caactgacct ttggcagtag
     5881 gtcgccaggc ggtgcgcttc atcttggcat caatgctctt ttcgatgatg cagttaaagg
     5941 ccgccgccgc gcccgctacc agccagatgc cgatacaggc cagcagcgcc ttacccgcct
     6001 gcggccagga cggcacaccg ggcaccgcca gcaccatgcc gatcagggca caaaatacga
     6061 tgagctggat cacgcgtggc ttggtcagcg cataaaactg ggcccatacc gacaaagtcg
     6121 tcgcggtcgc cgggccggtc ggcgcactca tggctgacgc ccggaaactg tcgggccgcc
     6181 tgcaggcagc actttgcgcc tgggtgccgc accagcgcca acccaggtta gcgtcagcac
     6241 cagggccgcc gcacccccgg tgtgcagcag cgcagccagc agcggccagc ccaacaccac
     6301 attggccaga ccggtggcca attgcaaacc gatcaatgct gccagcagac gggattgcag
     6361 tcgccatgca tttgaccggt tgagttgcca ggctagcagc atcagcaaac ccaacacgcc
     6421 ataggccagc agccggtgca tgtaatgaat ggcggtcagc gcggcaaaac tgatgggctc
     6481 gcccgagccc gtcaggccca ggtcacgcca gatttcaaac ccctgcgcca gattcatgtc
     6541 gggccaccaa ctgccctggc aggtcggg
//
LOCUS       DAOSLL010000214         6565 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10669_length_6565_cov_3.691091, whole genome shotgun sequence.
ACCESSION   DAOSLL010000214 DAOSLL010000000
VERSION     DAOSLL010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6565)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10669_length_6565_cov_3.691091"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /gene="nadB"
                     /locus_tag="PLB25_10480"
     CDS             complement(<1..1145)
                     /gene="nadB"
                     /locus_tag="PLB25_10480"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HOE42038.1"
                     /translation="MNPKNPNIAKPLAPLSFDVLIVGSGLAGLSAALLLSHNKKVAVI
                     TKRAVSEGSSGWAQGGIAAVWNKDDSFAAHVDDTLIAGAGLCDLAATRFVVENAPKAI
                     GWLQSMGVPFSEEDGQLHLTREGGHSARRIVHVTDATGAAVQRTLIDAVLKTPNITLF
                     EQHTLVDLITTDKLALPGKRCLGLYALDSQTDQVLTFEAPQTILATGGAGKVYLYTTN
                     PDTATGDGIAAAWRAGCRVQNMEFIQFHPTCLYHPHAKSFLISEAVRGEGGRLLLPDG
                     TRFMPQHDPRAELAPRDVVARAIDFEMKKGGFDCVYLDISHQPLSFLMEHFPNIYARC
                     LELGIDIAKQPIPVVPAAHYTCGGVLADLQGRTDVAGLYAIGETACSG"
     gene            complement(1154..2002)
                     /locus_tag="PLB25_10485"
     CDS             complement(1154..2002)
                     /locus_tag="PLB25_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HOE42039.1"
                     /translation="MVRSGTLAQSAEVALSGMPSVVDQVAVARLYGEPLFAMPQDLYI
                     PPDALEVFLEAFEGPLDLLLYLIRKQNFNILDIPMAGLTRQYLSYIDEIRHRNLELAA
                     EYLLMAAMLIEIKSRMLLPPKAVAQGQDEQDPRAELVRRLLEYEQMKQAAGRLNLLPQ
                     LGRDFLTAQVCIEQSLQPYFPEVSLSDLQEAWAGILRRAKLVQRHHISREELSVREHM
                     SMVLKRLQGQRFVEFEALFDPGKGMPVLVVTFIALLELAKEALVEINQAEAYAPIYVR
                     LAYTPS"
     gene            complement(2071..2280)
                     /locus_tag="PLB25_10490"
     CDS             complement(2071..2280)
                     /locus_tag="PLB25_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HOE42040.1"
                     /translation="MNIFYRPQYQSDITKFIDELKAKNPELEAAQRQGRSLLWDKAVD
                     RNAWTQYRAAQVEQQPYVYQTSGRE"
     gene            2408..4432
                     /gene="hyfB"
                     /locus_tag="PLB25_10495"
     CDS             2408..4432
                     /gene="hyfB"
                     /locus_tag="PLB25_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HOE42041.1"
                     /translation="MNSFTHPATWVHLDWVLLVVAGWLLIGLLGVAALRRLRLVAIVL
                     FPAGAFLSVLLMAVALSAAFAAPETAVLPLGLPQLPFHLRLDSLSAFFLLLIGAVSAG
                     VSAFAAGYFRHGEGTPPGLICLEYHLFLASIAMLVMADDAYVFMVMWETMALASFFLV
                     MANHRIPEIRQAGYLYMLVAHVGALGILLCFGLLQANTGDYTFANMRAQQLTPFWGSV
                     AFVLAVFGFGAKAGLLPLHAWLPEAHPAAPSPFSALMSGVMLKIALYGILRVSFDLLQ
                     TRIWWWGVLLMLVGLATALFGVVFSTVQVEMKRLLAYSSIENIGLMCAGMGLSLLFSA
                     YEMHALSALALTAALYHMISHAFFKSLLFCSTGAVMHATGERSLGRLGGLIRYMPWVA
                     WPTLVGVLACAGLPPFGGFVAEWLLLQSFLFTTGLPSSFLDMLVPVMAALIALIAALS
                     GYTMVKYFGVVFLGQPREEKLSKARDAGAWERLGMMWLVTGCLLLGLLPNQTIALIEP
                     VTRLLVHGGLTQTVNDSGWLLVPVHIDRASYAPAIFLLVVLASFGLSFLLVRRLYHGR
                     LRRVPPWDCGHPWQTARMQDTAEGLGQPIRRIFDSFFRITRQLPTAFDAQPHYQVTLE
                     DPLWYALYLPLARWVQRISDIVGLLQQGRIAVYLMYSFATLVVVLLLVKR"
     gene            4429..5379
                     /locus_tag="PLB25_10500"
     CDS             4429..5379
                     /locus_tag="PLB25_10500"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HOE42042.1"
                     /translation="MSALGIFSQLLALVTALLLAPLLSGWVNQWRHWLQNKSAPGVLQ
                     PYRMLHKLFNKESLMAEHASPLYRFAPYVIFSCMLLACAIIPTISTDLPLSPAADAIA
                     LVGLFALARVFISLAAMDIGTAFGSLGARREMLVGFLAEPALLMVLFCASMITRSTSL
                     TTIVETLAHRDLMIYPSLLLAGVAFTMVSLAENARVPVDNPDTHLELTMIHEALILEY
                     SARHLALLEWAASLKLFAYSCIGLALFFPWGVAEANAPLTLLMALPVLVFKLAAGGML
                     LAMIETASAKMRVFRVPEFLGTAFLLAVIGLLVNVLLGVG"
     gene            5376..6035
                     /locus_tag="PLB25_10505"
     CDS             5376..6035
                     /locus_tag="PLB25_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase"
                     /protein_id="HOE42043.1"
                     /translation="MIKFVPQLINLFATLILLLSFAMISQRRIVSLINLFMLQGAALV
                     AASFLLGYVTHQPDLYVSGLLTLVLKVVFIPWMLHRMINKLNVRWDVETLLNIPTTML
                     VGIALVIFAFSLALPVSRLSDSIAGGSLGIALACVFLSFMMMITRSKAVPQVIGFLSM
                     ENGLIFAATTVTNGMPMIVEFGIALDVLVGVLILGVFMFQIREKFDNLDIHNLEVLKE
                     D"
     gene            6038..>6565
                     /locus_tag="PLB25_10510"
     CDS             6038..>6565
                     /locus_tag="PLB25_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HOE42044.1"
                     /translation="MNALFFVLGIPLLGGLVLALVGHRAAARDVNVAFSLGTFLAACL
                     LTAQVIADGPQFVWGKQFYIDPLNVFLVTLTAFVGLTTAIFSRPYMRLEQDHGKMTVP
                     RMRLYHSMYQLFSFTMLLGLTTNNLGIIWVAMEAATLTTVLLVSVYRTTASLEAAWKY
                     FILCGVGIAQALFGTV"
BASE COUNT         1121 a         1880 c         1950 g         1614 t
ORIGIN      
        1 ccgctgcagg cggtttctcc aatggcgtaa agcccggcga catcggtacg accctgcaag
       61 tcggccagca cgccgccgca ggtgtagtgg gcagcgggca ccaccggaat cggctgtttg
      121 gcgatgtcga tgcccagctc caggcagcgc gcgtatatgt tgggaaagtg ctccatcaaa
      181 aaactcaagg gctggtgcga aatatcgaga tagacacagt caaagccgcc ctttttcatc
      241 tcgaaatcaa tcgcccgcgc caccacgtca cgcggtgcca gctcggcacg cgggtcatgc
      301 tgcggcatga accgggtgcc gtcgggcaac agcagacgcc cgccttcacc gcgcaccgct
      361 tcgctgatca ggaacgattt ggcatgcggg tggtacaggc aggtcggatg aaactggatg
      421 aactccatgt tctgcacccg gcaaccggcg cgccaggctg ccgcaatacc gtcgccggtg
      481 gcagtgtcgg ggttggtggt gtacagatag accttgccag caccaccggt agccagaatc
      541 gtctgtggtg cctcgaaggt cagcacctga tcggtctggc tgtccagcgc gtacaggccc
      601 aggcagcgct tgccgggcag cgccagcttg tcggtggtga tcaggtccac cagggtgtgc
      661 tgctcaaaca gggtgatgtt gggggttttc agcaccgcgt cgatcagcgt gcgttgtacc
      721 gcagcaccgg tggcatcggt cacatgcacg atgcgccggg cgctatgccc accttcgcgc
      781 gtcagatgca actggccgtc ttcttcagaa aacggcaccc ccatgctttg cagccagcca
      841 atcgccttgg gtgcgttttc caccacaaaa cgtgttgcgg ccagatcgca cagacccgct
      901 ccggcgatca gcgtgtcatc aacgtgggcg gcaaaactgt cgtccttgtt ccataccgca
      961 gcaatacccc cctgggccca gccactcgat ccttcggaaa cggcgcgctt ggtaatgacg
     1021 gcaactttct tgttgtggga cagcaacagt gcggcgctca atccggccag gccactgccg
     1081 acaatcaaga cgtcaaacga caggggcgca agcggttttg caatgtttgg atttttggga
     1141 ttcatagaaa agctcaggac ggcgtgtaag ccaatctgac ataaattgga gcgtaggcct
     1201 ctgcctggtt gatttcaacc agggcttctt tggcgagttc cagcagggca ataaaggtaa
     1261 ccaccaacac cggcatgcct ttgccagggt caaacaaggc ctcaaactca acaaagcgct
     1321 gcccctgcaa gcgcttgagc accatgctca tgtgttcgcg tactgacagt tcctcgcgac
     1381 tgatgtggtg tcgttgcacc agtttcgcgc gcctcaaaat acccgcccag gcctcctgca
     1441 ggtcgctcaa gctgacttca ggaaagtacg gctgcagcga ctgctcgata caaacctgcg
     1501 ccgtcaaaaa atcacggccc aactgcggca agaggttcag ccgccctgcc gcctgcttca
     1561 tttgctcgta ctccagcagg cggcgcacca gctcggcgcg tgggtcttgc tcgtcttgcc
     1621 cttgtgccac ggctttgggt ggcagcaaca tgcgcgactt gatctcgatc agcattgccg
     1681 ccatcagcag gtactcggct gccaactcaa ggttgcggtg gcgaatctca tcgatgtagc
     1741 tcaaatactg gcgtgtcagg ccagccatgg gaatatcaag aatgttgaaa ttctgtttgc
     1801 gaatcaggta cagcagcaaa tcaagcgggc cttcaaacgc ctccagaaac acttccagcg
     1861 catcgggcgg gatgtacaag tcctgaggca tggcaaacag tggttcacca tagagtcgtg
     1921 cgaccgctac ctgatccact acgctgggca tgccagacag ggcaacctcg gcagattgag
     1981 ccagcgttcc tgatcgcacc attgaaacca gattgctata gcaaaagtag ctgccagcgc
     2041 aggcagaaca agggctacag ggtcaaaagc ctattctctt ccggaggtct gatagacata
     2101 gggctgttgt tcgacttgcg cggcccggta ctgggtccat gcattccggt ccacagcttt
     2161 gtcccacagc agactgcgac cctggcgctg agccgcttca agctcagggt tcttggcttt
     2221 aagttcatca atgaacttgg tgatgtcaga ttggtattgg ggacggtaga aaatgttcat
     2281 ggtttgaagc cttttgctgg cattttagcc ccgcatccag aatgcagccg caagatcagg
     2341 ctcgatggcg gtaaatgcag ggtgtaatat ctcgcgctca cctgccttca cttcaatcaa
     2401 ccgattcatg aacagtttca ctcatccggc cacttgggtc caccttgatt gggtgctgct
     2461 ggtggtggct gggtggttgt tgattggtct gttgggtgtg gcagctttgc ggcggcttcg
     2521 cctggtggcc atcgtgctgt ttcctgccgg cgcctttttg tcggtgctgt tgatggcggt
     2581 ggctctcagt gccgcctttg ctgcgccaga gactgcggtg ctaccactgg gcttgccgca
     2641 gcttccgttt catttgcggc ttgacagtct gtcggccttt ttcctgctgt tgatcggtgc
     2701 tgtctcggcc ggcgtgtcgg cttttgcggc gggctacttt cgtcacggtg aaggcacccc
     2761 gcccggcctg atttgcctgg aataccacct gtttctggcc agcattgcca tgctggtgat
     2821 ggctgacgac gcttacgttt tcatggtgat gtgggaaacc atggcgcttg cgtccttttt
     2881 tctggtgatg gccaaccatc gcattccaga gattcgccag gccggctact tgtacatgct
     2941 ggtcgcgcat gtcggtgcct tgggcatttt gctgtgtttc ggtttgctgc aggccaatac
     3001 cggtgactac acgtttgcca acatgcgggc gcagcagttg accccgttct ggggttcggt
     3061 ggcctttgtg ctggcggtat ttggctttgg agccaaggcc ggtttgctgc cgttgcatgc
     3121 ctggttgccc gaggcacacc cggccgcacc ctcgcctttt tcagcgctga tgagtggtgt
     3181 catgctcaaa atcgcgctct acggcatctt gcgggtgtcg tttgacttgc tgcaaacccg
     3241 catctggtgg tggggcgtgt tgctgatgct cgttggcctg gccactgcct tgtttggcgt
     3301 ggtgttttcc accgtgcaag ttgagatgaa gcgcctgctg gcctattcgt cgatcgaaaa
     3361 catcggcctg atgtgtgctg gcatgggctt gtcactgctg ttctcagcct atgagatgca
     3421 tgcgctgtca gcgctggcgc tgaccgctgc gctttaccac atgatcagcc acgcgttttt
     3481 caagagcctg ctgttttgca gcactggtgc cgtgatgcac gccactggcg aacgcagttt
     3541 gggtcggctt ggtggcctga ttcgttacat gccgtgggtc gcctggccta cgctggtcgg
     3601 cgtgctggcc tgcgccggcc tgccgccgtt tggtggcttt gtcgccgaat ggctgctgct
     3661 gcagagtttt ttgttcacca ccgggctgcc aagttcgttt ctcgacatgc tggtgccggt
     3721 gatggcggcg ctgatcgcct tgattgcggc cttgtccggc tacaccatgg tcaagtattt
     3781 tggcgtggtt tttctgggcc agccgcgcga ggaaaaactg tcaaaggcgc gtgatgccgg
     3841 ggcatgggag cgactcggca tgatgtggct ggtgaccggt tgcctgctgc ttggcctgtt
     3901 acccaatcag accattgcgt tgatcgagcc ggtgacccgg ctgctggtgc acggcggact
     3961 gacccagacg gtgaacgata gcggctggct gctggtgccg gttcacattg accgggccag
     4021 ctatgcaccc gccatttttc tgttggtcgt actggccagc tttggcctgt cctttttgct
     4081 ggtgcgcagg ctctaccatg gtcgcctgcg ccgggtgccg ccgtgggact gcggccaccc
     4141 ctggcaaacc gcccgcatgc aagacacagc cgaaggtctg ggccagccga tccggcggat
     4201 ttttgattcg tttttccgca tcacccggca attgccaact gcttttgatg cgcaacccca
     4261 ttaccaggtg accttggaag acccgctctg gtatgccctt tatttgccgc tggcccgctg
     4321 ggtgcagcgc atttcagaca ttgtcggcct gttgcagcag ggtcgcattg cggtctatct
     4381 gatgtacagc tttgccacgc tggtggtcgt gctgctgctg gtcaagcgat gagcgcgctg
     4441 ggcatttttt cacaattgct ggcgctggtc acagcgctgc tgctggcacc attgctgtcg
     4501 ggttgggtca accaatggcg gcactggctg caaaacaaat ctgcgccggg tgtgttgcag
     4561 ccctaccgca tgctgcacaa actcttcaac aaggaatctt tgatggccga gcacgcctcg
     4621 ccgctgtacc gctttgcgcc ttacgtcatt ttcagttgca tgttgctggc ttgcgccatc
     4681 atcccgacca tctcgaccga tttgccgctg tcgccggccg ccgacgcgat tgcgctggtc
     4741 ggcttgtttg cgctggcgcg ggtgtttatt tcgctggcag ccatggacat tggcaccgcc
     4801 tttggttcgt taggcgcgcg gcgcgaaatg ctggtgggtt ttctggcaga gccagccttg
     4861 ctgatggtgc tgttttgcgc ctccatgatc acccgctcaa cctcgctgac caccattgtg
     4921 gagactttgg cgcaccgtga tctgatgatt taccccagcc ttttgcttgc cggggtggct
     4981 ttcaccatgg tgtcgctggc cgaaaacgcc cgtgtgccgg tcgataaccc tgacacccac
     5041 cttgaactca ccatgatcca cgaggctctg attctggagt attcggcccg ccacctggcg
     5101 ctgctggaat gggccgccag cctgaagctg tttgcctatt cgtgcatcgg cctggcgctg
     5161 ttttttcctt ggggcgtggc cgaggccaat gcaccgttga cgctgctgat ggcgctgccg
     5221 gtgctggtgt tcaagctggc agcaggtggc atgctgctgg ccatgattga gaccgccagc
     5281 gccaaaatgc gggtgtttcg tgtgcccgag tttttaggca ccgccttttt gctggcggtg
     5341 attggtttgc tggtcaatgt gctgctgggg gtcggatgat caagtttgta ccccaactca
     5401 tcaacctgtt tgccacgctg attctgctgc tgtcgtttgc catgatctcg cagcggcgca
     5461 ttgtgtcgct catcaacctg ttcatgctgc aaggagccgc gctggtagcc gcctcgtttt
     5521 tgctgggtta tgtcacgcat cagccagatc tgtatgtatc agggctgctg acgctggtgc
     5581 tcaaggtggt gtttattccg tggatgctgc accgcatgat caacaaactc aacgtgcgct
     5641 gggacgtgga aaccctgctc aacatcccga ccaccatgct ggtcggcatt gcgctggtga
     5701 tttttgcgtt cagcctggcg ctgccagtgt cgcgcctgtc cgactcgatt gctggcggct
     5761 cgctgggcat tgcgctggcc tgtgtttttc tgtccttcat gatgatgatc acccgctcca
     5821 aagccgtacc gcaggtgatt ggcttcttgt caatggaaaa cgggctgatc tttgccgcta
     5881 ccaccgtcac caacggcatg ccgatgattg ttgaatttgg cattgcactt gacgtgctgg
     5941 ttggcgttct gattcttggg gttttcatgt ttcagattcg cgagaaattt gacaacctcg
     6001 acattcacaa ccttgaagtg ctcaaggaag actgagcatg aacgcacttt ttttcgtact
     6061 gggaattccg ctgctcggtg gcctggtgct ggccctggtc gggcatcgcg ctgcggcgcg
     6121 ggatgtgaat gtggcgttca gccttggcac ttttctggca gcctgtttgc tgacggcgca
     6181 ggtgattgcg gatggcccgc agtttgtatg gggcaagcag ttctacatag acccgctgaa
     6241 tgtttttctg gtcaccctga cggcttttgt tggcctgacc accgccattt tttcacggcc
     6301 ctacatgcgc ctggagcaag accacggcaa gatgacagtg ccacgcatgc ggctgtacca
     6361 cagcatgtac cagttgttca gcttcaccat gctgctgggc ctgaccacca acaatctggg
     6421 catcatctgg gtggcgatgg aagccgccac gctgaccacc gtgctgctgg tcagcgtcta
     6481 tcgcaccacc gccagcctgg aggcggcctg gaaatatttc atcttgtgtg gtgtcggcat
     6541 tgcgcaggcg ctgtttggca cggtg
//
LOCUS       DAOSLL010000215         6564 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10672_length_6564_cov_4.960977, whole genome shotgun sequence.
ACCESSION   DAOSLL010000215 DAOSLL010000000
VERSION     DAOSLL010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10672_length_6564_cov_4.960977"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /gene="pgk"
                     /locus_tag="PLB25_10515"
     CDS             complement(<1..776)
                     /gene="pgk"
                     /locus_tag="PLB25_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HOE42045.1"
                     /translation="MTVIRFSDLCAQGQVAGKRVFIRADLNVPQDDAGNITEDTRIRA
                     SVPCIQMALDAGAAVMVTSHLGRPTEGEFKPEDSLAPVAKRLGELMGRDIPVVANWVD
                     GVDVKPGQLVMLENCRVNKGEKKNNEELAKKMAALCDVFVHDAFGTAHRAEGTTYGIA
                     QFAPIACAGPLLSAEIDAINLALANPKRPLVAIVAGSKVSTKLSILQALAKNVDQLIV
                     GGGIANTFMLAAGLKIGKSLAEPTLVSEATAVIAAMKARGA"
     gene            complement(957..3854)
                     /locus_tag="PLB25_10520"
     CDS             complement(957..3854)
                     /locus_tag="PLB25_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HOE42046.1"
                     /translation="MSEINVPPMKLSGLEPVIIGAAPAADAALGQAPSATFVNIGERT
                     NVTGSKAFARMILNGEFEQALSVARQQVENGAQIIDINMDEAMLDSQAAMVRFLNLIA
                     SEPDISRVPVMIDSSKWSVIEAGLRCVQGKPVVNSISMKEGVDAFKHQARLLRRYGAA
                     AVVMAFDELGQADTYQRKIDICQRAYRILVDEVGFPPEDIIFDPNIFAIATGIEEHNN
                     YAVDFIEATRWIKQHLPGAKVSGGVSNVSFSFRGNDPVREAIHTVFLYHAIKAGMDMG
                     IVNAGMVGVYDDLEPVLRERVEDVVLNRRPRIAEGEPEQSAAERLVDIAASAKGAARD
                     ESRKLEWRGSAAQPVSVEQRLSHAMVHGITDFIVADTEEAYQAIIARGGRALQVIEGP
                     LMAGMSVVGDLFGQGKMFLPQVVKSARVMKSAVAHLIGYIEEEKRLDQAVGRDVATKG
                     KIIMATVKGDVHDIGKNIVTVVLQCNNFDVVNMGVMVPCHDILARAKLEGADMIGLSG
                     LITPSLEEMQYVASEMQKDDYFRLRQMPLLIGGATTSRVHTAVKIAPHYDGPVVYVPD
                     ASRSVGVAQSLLAERASSFIAELNADYARVRTQHANKKQTPLWPLAKARANKTPIDWL
                     ACPPTRPKFIGRRVFKNFDLSELAGFIDWGPFFQTWDLAGPFPAILQDKVVGAEATKV
                     YADGRAMLKRVIEGRWLTANAVLGLYPANSVNHDDIEIYTDESRSTVAMTWYGLRQQA
                     AKEEIDGQMMPSRCLADFVTAQQNATEKVAPGAYDTGAGGQKDLKMRGSAEASAAASV
                     GDYIGLFAVTAGLNIEQKEAQFLAAHDDYSAIMLKALADRLAEAFAECLHHRVRTDLW
                     GYAPSEALSPAELIAEKYVGIRPAPGYPACPDHSAKKDLFALLQCEEIGMTLTDSLAM
                     RPAASVSGFYLGHPQACYFNVGKIGDDQLKDMALRRGMTEPALQRLLAPNL"
     regulatory      complement(3881..3952)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
     gene            4075..5394
                     /locus_tag="PLB25_10525"
     CDS             4075..5394
                     /locus_tag="PLB25_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42047.1"
                     /translation="MHVNLSEALALLCVDDLKGLTRHLPGPPVTGRKADLVAAIATAM
                     LGAELPLLWKKLDETQKAAVAEAVHDPLGEFSSEIFEAKYKCLPAFVQGKGELRSSSR
                     NSTPLGLFIHKMVNGRNPGVPADLCRRLLEFVPEPAAAQIKSSRELDAVEGQAIRLTE
                     LDALQELTLMLRTIPKERITVGEKTARPSGAAQRLLTARLPNGDFYPWPEGSDKNEQQ
                     VGPIKAFAWPLLLQAGGLATRIGSRLALSPAGNKAVNAPPAEVIRTLWRKWQKTRLLD
                     EFSRVDDIKGQNSAGRVMSAVAPRRDAIEEALQACPVGEWIEVHEFSRFMQASDLRFV
                     IAHNPWKLFLADRQYGSLGYAGCNGWNILQHRYILALLFEYAATLGLLDVAYLDPTAP
                     GGGDDDFRSLWGADDLTFLSRYDGLRHFRVNALGAYALGLTDRYQPG"
     gene            5590..>6564
                     /locus_tag="PLB25_10530"
     CDS             5590..>6564
                     /locus_tag="PLB25_10530"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807173.1"
                     /note="catalyzes the formation of spermidine from
                     putrescine and S-adenosylmethioninamine; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HOE42048.1"
                     /translation="MTTTPSAVPTPRPLEVALLASVFVVAACGLVYELAAGALASYLL
                     GDSVLQFSTIIGSYLFAMGLGSYLSRFFERQLPAHFLRIELLVALIGGALPALLFVAN
                     ATLPGAFRGLLYGLVLLVGTLVGLEIPLVMRILKRNVALKDLVSQVLTFDYLGALAVS
                     LAFPLVLVPQLGLIRTGLLFGLMNASVAVWALWLFRHELRQWSAHAMACGLTLATLGA
                     GMAGAESLTTWTEGKLYQDLVVLAQTTPYQRIVVTHAPGAGRAGYRLFLNGNLQFAQR
                     DEYRYHEALVHPVMAAYAAAGSPRKVAVLGGGDGMAVREILKYPGVESI"
BASE COUNT         1215 a         2007 c         1968 g         1374 t
ORIGIN      
        1 gcaccacggg ccttcatggc ggcgatgaca gctgtggcct cactcaccag cgtgggttcg
       61 gccaggcttt tgccaatctt caggcccgca gccagcatga aggtgttggc aataccgcca
      121 cctacgatga gctggtccac attcttggcc aaggcttgca gaatgctgag cttggtggac
      181 accttggagc ccgccacaat cgccaccagt gggcgtttcg ggttggccag agccaggtta
      241 atggcgtcga tttcagccga caacaagggg ccagcgcagg caatgggggc aaactgggcg
      301 ataccgtagg tggtgccttc ggcgcggtgc gcggtaccaa aggcgtcatg cacaaacacg
      361 tcacacaagg cggccatttt cttggccagc tcttcgttgt ttttcttctc gcccttgttg
      421 acgcggcagt tttccagcat caccagttgg ccaggtttga catccacgcc gtcaacccag
      481 ttggccacca cgggaatgtc gcggcccatc agctcgccca ggcgcttggc cacgggagcc
      541 agggagtctt cgggtttgaa ctcgccttcg gtgggacggc ccaggtggct ggtaaccatc
      601 acggcagcac ctgcgtcaag cgccatctgg atacagggca cactggcgcg gatgcgggtg
      661 tcttcggtga tgttgcctgc gtcatcctgc ggcacattca ggtcagcacg gatgaagacg
      721 cgtttgccgg ccacctggcc ctgggcgcac aagtcagaaa atcggatgac agtcatggtc
      781 gtctttggag ttattgcaat ctggccgatt gtaaaagctg tgctggctgg tttcgttacc
      841 gcttgaagca tggcaatgag tccgacccaa accgggggaa caatttcggc gccaacaccg
      901 gtacctgaaa ttcgccaagg ccatccaggc tggtggatgc caggaccgcc tgcccgctac
      961 aaattcggtg ccagcaggcg ctgcagcgct ggctcggtca tgccacggcg cagcgccatg
     1021 tctttgagct ggtcgtcgcc tatcttgccg acattaaagt agcaggcctg cgggtggccc
     1081 aggtagaaac cgctgacact ggcagccggg cgcatggcca ggctgtcggt cagcgtcatg
     1141 ccgatttctt cgcattgcag cagcgcaaac aggtctttct tggcgctgtg gtcggggcag
     1201 gccgggtagc cgggggctgg gcgaatgccg acgtattttt cggcaatcag ctctgccggg
     1261 ctcagtgctt cggatggcgc gtagccccac aggtcggtgc gaacccggtg gtgcagacat
     1321 tcggcaaaag cttcagccag gcggtcggcc agcgccttga gcatgatggc gctgtagtcg
     1381 tcatgcgccg ccagaaactg ggcttctttt tgttcaatgt tcagaccggc cgtcaccgcg
     1441 aagagcccga tgtagtcgcc gacgcttgcg gcagcgctgg cctcggcgct gcctctcatt
     1501 tttaagtctt tttggcctcc agcccccgtg tcatatgcgc caggagctac cttttcagta
     1561 gcattttgtt gtgccgtcac aaagtccgcc aggcagcggc tgggcatcat ctggccgtct
     1621 atttcttctt tggcggcttg ctggcgcagg ccgtaccagg tcatcgccac cgtgctgcgc
     1681 gactcgtcgg tgtagatctc gatgtcgtcg tgattgaccg aattggccgg gtacaggcca
     1741 agcaccgcat tggcggtgag ccagcggccc tcaatcaccc gcttgagcat ggcacggcca
     1801 tcggcgtaga ccttggtggc ctctgcaccg accactttgt cttgcagaat agccggaaac
     1861 ggcccggcca ggtcccaggt ctggaaaaac ggaccccagt cgatgaaacc agccagttcg
     1921 ctcaggtcaa agtttttgaa gacccgccga ccaatgaact tcggccgagt cggcgggcaa
     1981 gcaagccagt caatcggtgt tttgttggcg cgcgctttgg ccaacggcca cagcggggtc
     2041 tgctttttgt tcgcgtgctg ggtgcgcacc ctggcgtaat cagcgttcaa ctcggcaatg
     2101 aagctgctgg cccgttcggc gagcaggctt tgcgccacac caacgctgcg cgacgcatca
     2161 ggcacataaa ccaccgggcc gtcgtaatgc ggcgcgatct tgacagcggt atgcacccgg
     2221 ctggtggtgg caccaccaat cagcaacggc atctggcgca accggaagta atcgtccttt
     2281 tgcatctcgc tggcgacata ttgcatttct tccaggctcg gcgtgatcag gcccgacaag
     2341 ccaatcatgt cggcgccctc cagcttggca cgcgccagga tgtcgtggca cggcaccatc
     2401 acgcccatat tcaccacatc aaagttgttg cactgcagca ccacggtgac gatgtttttg
     2461 ccgatgtcat gcacatcgcc cttgacggtg gccatgatga tcttgccctt ggtcgccaca
     2521 tcgcggccaa cagcctggtc tagccgcttt tcttcttcaa tgtagccaat caggtgcgcc
     2581 accgccgatt tcatgacccg cgcgcttttc accacctgcg gcaaaaacat cttgccctgg
     2641 ccaaacaggt cgccaacgac actcatgcca gccatcagcg ggccttcaat cacctgcagc
     2701 gcgcgaccac ccctggcaat gatcgcctgg tacgcttctt cggtgtcagc cacaatgaaa
     2761 tcggtgatgc catgcaccat ggcatggctc aggcgctgct cgacgctgac cggttgcgcg
     2821 gcgctgccac gccattcgag ttttctggat tcatccctgg cggcaccttt ggcgctggcg
     2881 gcaatgtcta ccagacgctc tgcggcggac tgctcgggct cgccctcggc gatgcgtggc
     2941 cgacggttca gcaccacatc ttcgacccgc tcgcgcaaca ctggctcgag gtcgtcgtaa
     3001 acgcccacca tgccggcatt gacaatgccc atgtccatgc cggccttgat cgcgtggtac
     3061 aaaaacaccg tgtgaatcgc ttcacgcacc gggtcgttgc cacggaagct gaacgacaca
     3121 ttcgacaccc cgcccgacac cttggcgccc ggcaggtgct gcttgatcca gcgcgtggct
     3181 tcaatgaagt caaccgcgta gttgttgtgc tcttcaatgc cggtggcaat ggcaaagata
     3241 ttcgggtcaa agatgatgtc ttccggcgga aaaccgacct catcgaccag gatgcggtag
     3301 gcccgctggc aaatgtcgat cttgcgctgg taggtatcgg cctggccgag ctcgtcaaac
     3361 gccatcacca ccgctgcggc accatagcgg cgcaacaacc tggcctggtg cttgaaggca
     3421 tcgacccctt ccttcatgct gatcgagttg accaccggct tgccctgcac acagcgcagg
     3481 ccggcttcga tcacgctcca cttgctcgaa tcaatcatca ccggaaccct tgatatgtct
     3541 ggctcagacg ctatcaaatt caggaaccgc accatcgctg cctggctgtc gagcatggcc
     3601 tcgtccatgt tgatgtcgat gatctgggca ccgttttcaa cctgctggcg cgccacgctc
     3661 agggcttgtt caaactcgcc attgagaatc atccgggcaa aagccttgga gccagtgaca
     3721 ttggtgcgct caccaatatt gacgaacgtg gcgctcgggg cctgccccaa ggcagcgtca
     3781 gcagcagggg ctgcgccaat aatgaccggc tccaggccag acagcttcat ggggggaaca
     3841 ttgatttcag acatgcgact cacctgtggg caaacgattg atcaggtgag cgtcgttgag
     3901 cacgatccaa gcctgacggg ccgtgtagcc cgctgcaacg ctccttggaa gagcggattt
     3961 taccggctgc tgcccggccc ggtcgccagc cagcgttttg caagcacaat tcaatcacgc
     4021 aagaataacc gccaggctgg cctggataca ccactttttt ccgcaggatt tctcatgcac
     4081 gtcaatttgt ccgaagctct cgcgctgctt tgcgtggacg atctcaaagg cctgacccgt
     4141 cacctgccag gcccgccggt gaccgggcgc aaggccgatc tggtggcggc aatcgcgact
     4201 gcaatgctgg gtgccgagtt gccgttactt tggaaaaaac tcgatgaaac ccaaaaggcg
     4261 gcagtggcag aggcggtaca cgacccgctg ggtgagttct caagcgaaat ttttgaagcc
     4321 aaatacaagt gcctgccagc ctttgtgcag ggcaaagggg aactgcgcag cagcagccgc
     4381 aacagtaccc cgctgggttt gttcattcac aaaatggtca acgggcgcaa ccctggcgta
     4441 ccggcagatt tgtgtcggcg cctgcttgag ttcgtgcccg agccagccgc cgcgcagatc
     4501 aaatccagcc gggaactcga tgcggttgag ggccaggcaa tccgcctgac cgaacttgat
     4561 gccttgcagg aactgacgct gatgttgcga accatcccca aggagcgcat cacggttggg
     4621 gaaaagaccg cccggcccag cggcgcggcc cagcgcttgc tgacggcccg gctgcccaat
     4681 ggtgacttct acccctggcc tgaaggcagc gacaaaaatg agcagcaggt cggcccgatc
     4741 aaggcctttg cctggccact gctgttgcag gccggtgggc tcgcgacccg catcggcagc
     4801 cgactggcgc tgagcccggc cggcaacaag gcggtcaatg cgccaccagc cgaggtgatt
     4861 cgcacgctgt ggcgcaaatg gcagaaaact cgcttgctcg acgaattcag ccgggtcgat
     4921 gacatcaagg gacaaaacag cgctggccgg gtgatgagtg cggtagcgcc ccgacgtgac
     4981 gcgattgaag aagccttgca agcctgcccg gtgggcgagt ggattgaggt gcatgagttt
     5041 tccagattca tgcaggccag cgacctgcgc tttgtcatcg cccataaccc ctggaagctg
     5101 ttcttggctg atcgccagta cggcagcctt ggctatgcgg gctgcaacgg ctggaacatc
     5161 ttgcaacacc gttatatttt ggcgctgctt tttgagtacg cggccaccct cgggttgctg
     5221 gatgtggcat accttgaccc aactgcgcct ggcggtggcg acgatgactt tcgcagcctg
     5281 tggggtgccg acgatctgac gtttttaagc cgttacgacg gcttgcgcca ctttcgggtg
     5341 aatgcgctgg gtgcctatgc attgggcctg accgaccgct atcagccagg atagcaggca
     5401 agcccgacag acaccgcagc ctgcccccat ttttgaacaa tagcctggcg ttggcggctg
     5461 ctgtgccagc atgcgtcgtt caagcgtggc ggttggggtg cttgccaacg cttgcgcatc
     5521 ttgcgccacc cagtccagca gcagtgcaag catcacaata gctgtctgcc ctaccttcca
     5581 cttcagccga tgaccacaac acccagtgcc gttcccacac cacgcccgct tgaagtcgcg
     5641 ctgctggcct cggtgtttgt ggtggccgcc tgcgggctgg tgtatgagct ggcggccggg
     5701 gcgctggcct cgtatctgct gggcgactcg gtactgcagt tttccaccat catcggcagc
     5761 tacctgtttg cgatggggct gggctcttat ttgtcgcgct ttttcgagcg ccagcttccc
     5821 gcccattttt taaggataga actgcttgta gcccttattg gtggggcgct accagctctc
     5881 ttatttgtag caaatgcaac attgccaggg gccttcaggg ggctgctgta cggtctggtg
     5941 ctgctggtcg gcacgctggt cgggctggag attccgctgg tgatgcgcat cctgaaacgc
     6001 aatgtggcat tgaaagacct ggtgtcgcag gtgctgacct ttgactatct gggtgcgctg
     6061 gcggtgtcgc tggcctttcc gctggtgctc gttccccaac ttggcctgat tcgtaccggg
     6121 ctgctgtttg gcctgatgaa cgccagcgtg gcggtgtggg cgctgtggct gtttcgccat
     6181 gagctgcgcc agtggtcagc gcatgcgatg gcctgtggct tgactttggc caccctaggc
     6241 gctggcatgg ccggtgccga gtcgctgacc acctggaccg aaggcaagct ctaccaggac
     6301 ctggtggtgt tggcccaaac cacgccatac cagcgcattg tggtcaccca cgcacccggc
     6361 gcggggcgcg ctggctaccg gctgtttctc aatggcaacc tgcagtttgc ccagcgcgat
     6421 gagtaccgct accacgaggc gctggtgcac ccggtgatgg ccgcctatgc cgccgctggt
     6481 tcccccagaa aggtagcggt gctgggcggc ggcgacggca tggcggtgcg tgaaattctg
     6541 aaatatcccg gtgtcgaaag catt
//
LOCUS       DAOSLL010000216         6480 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10828_length_6480_cov_4.657588, whole genome shotgun sequence.
ACCESSION   DAOSLL010000216 DAOSLL010000000
VERSION     DAOSLL010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6480)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6480)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6480
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10828_length_6480_cov_4.657588"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            6..1100
                     /locus_tag="PLB25_10535"
     CDS             6..1100
                     /locus_tag="PLB25_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE42049.1"
                     /translation="MAKHYGAFRAVDDLSLEVARGSFFSILGPSGCGKTTLLRMIAGF
                     LEADSGDILIGGKPMNGVPPNKRPVNMVFQHLALFPMMNVGDNVGYGLARRGVAKDEI
                     KRRVGNMLERVSLSGAQNKRVDQLSGGQKQRVAIARSLVLEPTLLLLDEPLGALDLKL
                     REHMKIELKQLQAVFGTTFVYITHDQSEALVLSDHVAVMNQGKFEQVGTPQQLYYEPQ
                     TPFVAGFVGANNRMAGRATSVLGEQVSVTTAKGLVIPARAIGKLAQGDAVEAFVRPEV
                     ARLVQDAAVLADAGLPSYSARVESLLFDGANSAVLLQEAVTQTEFRIALPQTGEFAHL
                     SVGQAVTFGFDPQRVVCFAAGTSSNAHVFS"
     gene            1087..1977
                     /locus_tag="PLB25_10540"
     CDS             1087..1977
                     /locus_tag="PLB25_10540"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE42050.1"
                     /translation="MSLAETPDVSRNSARLMLMLLLAPALLWLLGLIVLPHLELGILS
                     LRHRVAPRVYEPSLAQFATFIDEPLYWHTFVRTALMSIVATAITLLMAFPIAWTIAKI
                     ARGRVKSMMFVMCLIPFWVSETVRTLGWMILLRESGVLPALLVGLGVTPAPIEMLYHD
                     ATILVGLVYTSMLFMVIPLISALESLDDSLIEAAYDLGGNGWSILRQIVIPHAAPGIV
                     AGCIVVFMLTLGNYLTPTLLGGKNSQWFTEQIYTQFITRFNWEQGAAFGFLLLGLSTA
                     IVWAGLKLSGQKFGEVMQRS"
     gene            2081..2953
                     /locus_tag="PLB25_10545"
     CDS             2081..2953
                     /locus_tag="PLB25_10545"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE42051.1"
                     /translation="MIASLPRPLWLRTSTMLYGVAFFAFLFLPLAVVAVFAFNDASYP
                     APPWRGFTLDWFLGGTDAKRLGLFADAAMLGSIWTSVVVACWVTVLSVLVGTANAFLI
                     ERTQFRGKQALSMLMLAPLVIPGVILGISILAFASGIANQADDLWGLELDFLRPGLPL
                     VVLGQFSYIVSIATLTITARLKRFDVTLEEAAYNLGASRAAVLWTITLPYLKPAMIGS
                     AAISFLMSFENFNTTLMLVGSDAPLTVMMYGRMREGATPVLNAVSLLLMVSSAVLALT
                     MMRGPKGNASSHSD"
     gene            complement(3638..4960)
                     /locus_tag="PLB25_10550"
     CDS             complement(3638..4960)
                     /locus_tag="PLB25_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42052.1"
                     /translation="MIESFRRMRVSTSVFPVYVSLRYLPLLQSDGNEYIGHFCILLSQ
                     AIQKELSDRKLEHDFAEATDQVSLQFGLTALAQALNMRIVLLFDDAAHIGREKPLEVF
                     FDLYRTLSSSLTSCKASIYPGVTKFGVRFDVFNDSTVIDIGRSDTASAPGFFPDVVKA
                     RYPRLSERATFSDRLDPSQFANLLGRAVVGNLRGFILACNRFDNKDKIGIPEVSQCLL
                     DMASDYFWPLMDEVAPKLGIYEALVDPAREVFGVIVDHATKTVKSNTRNTAQDRILIH
                     RQTVSQFLKIFEILEYLGFVARREASRALKSGGRGPIFAINLCSLLEATPTRRLTSEM
                     IEEWLQGTSEPAELHISGTAFHAIKLPQLQDETGLAILDKEIKVLGRSPAYPYGLTDS
                     IIQRLSTAGMTTVGQLANATDKELDDIEYIGEVKIQVIRDVVFQAIWM"
     gene            complement(5140..5919)
                     /locus_tag="PLB25_10555"
     CDS             complement(5140..5919)
                     /locus_tag="PLB25_10555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42053.1"
                     /translation="MRISFVPTLDQMNEIALLLCGDNSEGTGKIALDQLASSLRSFSA
                     KAATFLTEDITDEALSFGPFCSRVLLENGCAALVGRLDSFRMLYLSEFQAQPEYEPGK
                     RAKSAFAWSGDVIPDEKPNQALWSIDHDLPKISRALFSKYFEHIFWKPAINRMLDYVS
                     SLESDPLLADVANIDPDRFIDETKGRSIQLYSTLSKGVHWEFFTSALLFDEATVKNSI
                     RETFLLVANLGLVSHFVHTAYASLAPDEAARCYKAIRRLVP"
     gene            complement(6169..>6480)
                     /locus_tag="PLB25_10560"
     CDS             complement(6169..>6480)
                     /locus_tag="PLB25_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010397060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42054.1"
                     /translation="ARHPYDALILCEPDFAFVQDGTRRDDGFRQRQHDWYLTQLQNVH
                     CPVLRLDKDVPTRVRNAAHWLNRVLGVHASASQEADRPASPSPTWLAQRMLPPGPISN
                     R"
BASE COUNT         1333 a         1737 c         1879 g         1531 t
ORIGIN      
        1 cccgcgtggc caagcactac ggtgctttcc gcgcggtgga tgatttgtcg cttgaggtgg
       61 cgcgcggcag ttttttctcg attctggggc cttctggctg cggcaaaacc acgctgctgc
      121 gcatgattgc aggttttctg gaagccgact ccggcgacat cctgattggt ggcaagccca
      181 tgaatggcgt gccgcccaac aagcgcccgg tcaacatggt gtttcagcac ctggcgctgt
      241 ttccgatgat gaatgtgggc gacaacgtcg gttacggcct ggcgcgccgt ggtgtcgcca
      301 aggatgaaat caagcgccgt gttggcaaca tgctggagcg ggtcagcctg tctggtgcgc
      361 agaacaaacg ggtcgatcag ctctcgggcg gacaaaagca gcgtgtggcg attgcccgca
      421 gcctggtgct cgaacccacg ctgctgctgc ttgatgagcc gctgggtgcg ctggacctta
      481 aattgcgcga gcacatgaag attgagctca agcagttgca ggcagtgttt ggcaccacct
      541 ttgtctatat cacccacgac cagtccgagg cgctggtgct gtcagaccat gtggcggtga
      601 tgaaccaggg caagtttgaa caggtgggca caccgcagca gttgtattac gagccgcaaa
      661 ccccgtttgt ggcgggtttt gtcggggcca acaaccgcat ggccgggcgc gccacctctg
      721 tcttgggcga gcaggtcagc gtgaccaccg ccaaaggcct ggtgataccg gcgcgcgcca
      781 ttggcaaact ggcgcagggc gacgcggttg aggcctttgt ccgccccgaa gtcgcccgtc
      841 tggtgcagga tgcggcggtg ttggccgatg ccgggctgcc aagttacagc gcgcgggttg
      901 agagcctgct gtttgacggt gccaactcgg ccgtgctact gcaagaggca gtcacgcaaa
      961 ccgagtttcg catcgccttg ccacaaaccg gtgagttcgc ccacttgagc gtgggccagg
     1021 cagtgacatt tggctttgac ccgcaacgcg tggtgtgttt tgccgctggc accagctcca
     1081 acgcccatgt ctttagctga aacccctgac gtgtcgcgta actcggcgcg gctgatgctg
     1141 atgctgctgt tggcgccggc gctgttgtgg ctgctcgggc tgatcgtgct gccgcatctg
     1201 gagctgggca ttttgtcgct gcgccatcgg gtggcgccaa gggtgtacga gcccagtctg
     1261 gcgcagttcg ccacctttat tgacgagccg ctgtattggc acacctttgt gcgcactgcg
     1321 ctgatgtcga ttgtggccac cgccatcacg ctgctgatgg cttttccgat tgcctggacc
     1381 atcgccaaga ttgcccgggg tcgggtcaag tcgatgatgt ttgtgatgtg cctgatccca
     1441 ttctgggtga gtgagacggt gcgcacgctg ggctggatga tcttgctgcg cgagtccggc
     1501 gtgttgcccg cgctgctggt tggcctgggc gtaaccccgg cacccatcga gatgctgtac
     1561 cacgatgcca ccattctggt cggactggtc tatacctcga tgctgttcat ggtgattccg
     1621 ctgatcagcg cgctggaaag cctggacgac tcgctgattg aagccgccta cgacctgggc
     1681 ggcaacggct ggtcaatcct gcgccagatc gtcatcccgc atgctgcgcc cggcattgtg
     1741 gcgggctgca tcgtggtgtt catgctgacg ctgggcaact acctgacacc cacgctgctg
     1801 ggtggcaaaa actcgcaatg gttcaccgag cagatttaca cccagttcat cacccgcttt
     1861 aactgggagc agggcgcggc gtttggcttt ttgctgctgg gcctgtcaac cgccatcgtc
     1921 tgggctggcc tcaaactcag cggtcaaaag tttggcgaag tgatgcaaag gtcttgatca
     1981 tgcctggttg cccattgaat gctcttgaat caatagctgt cagcgcatgt gctgcctggg
     2041 ctggaggcca atttcattca actattggtg ggtcagcgcg gtgattgcat ccttgccccg
     2101 cccgctgtgg ttgcgcacca gtacgatgct gtacggtgtg gcgttttttg ccttcctgtt
     2161 cctgccactg gcagttgtgg cggtatttgc cttcaatgac gcttcctacc cagcgccacc
     2221 gtggcgcggt ttcacgcttg actggttcct gggtggcacc gatgccaaac ggctcgggct
     2281 gtttgccgat gccgccatgc tcggcagcat ctggaccagc gtggtggtgg cctgctgggt
     2341 gaccgtgctg tcggtgctgg tcggcaccgc caacgcgttt ttgatcgagc gcacccagtt
     2401 tcgcggcaag caggcactgt cgatgctgat gctggcacca ctggtgattc cgggggtgat
     2461 tctgggcatt tcgatactgg ctttcgccag tggcattgcc aaccaggctg acgacctgtg
     2521 ggggctggaa ctggactttt tgcgccccgg cctgccgctg gtggtgctgg gccagttttc
     2581 gtacattgtg tcgattgcca cgctgaccat caccgcgcgg ctcaaacgct ttgacgtgac
     2641 gctggaagaa gccgcctaca acctgggtgc atcgcgcgca gcggtgctat ggacgattac
     2701 cctgccgtac ctgaaaccgg cgatgattgg ctcggcggca atttcgtttt tgatgagttt
     2761 cgaaaatttc aacaccacct tgatgctggt cggctctgac gcgccgctga ccgtgatgat
     2821 gtatggccgc atgcgcgagg gtgccacgcc ggtgttgaac gcggtgagct tgctgctgat
     2881 ggtcagctct gccgtgctgg cgctgacgat gatgcgcgga ccaaagggca atgcgtcaag
     2941 ccattctgac tgaacggcga tctggttttg atgccaactt tggagccgtc aatgagtcgt
     3001 caatgagtcg tcaatgatgc cgggtcgctg gcatttcaca gacacatttg gctttgatcg
     3061 gtcagcaaca ggcggttttc cagaagccga ttttctggaa agcctcagct cgtccagtcg
     3121 ctggaatttg ttgtcagata aaagttctgt ttcttgcggg tgattcatgc cgtgccgacg
     3181 gggtctgtgc gcctggtgtc tggtggtgcg acgagctggc gctgcgcagc agccggacag
     3241 ggcggcccct catactggag taccagaaat cggggccgcc ctgtccggcc gctacgccga
     3301 tgccattgat tgtgcgcaag ggcggtttga ataccgcaag ctgtgggtca acgggggttt
     3361 gccggacgtt tggcggatgg tgctggtagg gacagtgccg agtgcctggc gtttatatgt
     3421 caaattggcc tgtagccccc gttgtatatg cgtcgctagc tctttagttt atagcagata
     3481 ggttgcttgt tgaatcgagg gtgacaggct tctggagacc cgacagcgac atttgacttt
     3541 cagcgaccat cggcaggact cgacccgaag cggaccttca gatgggtgga aatacagacc
     3601 caaagcagac ccttgaaatc agctgcattc cgctgggcta catccaaatg gcttgaaaca
     3661 ctacgtcgcg gataacttga atcttcacct cgccgatata ttcaatgtca tcaagctcct
     3721 tgtctgtcgc attggcgagt tgccccacag ttgtcatacc ggctgtactc aagcgttgaa
     3781 tgatgctatc ggttagacca tatggatagg ctggcgacct accgagcacc ttaatttcct
     3841 tatcaaggat cgccaaccca gtctcatctt gcagttgtgg caactttatt gcatgaaacg
     3901 cagttccaga aatatgcaac tcggcaggct cagatgtacc ttgtagccac tcctcaatca
     3961 tttcagaagt caggcgcctc gttggggttg cttcaagtag gctgcataga ttgatcgcaa
     4021 agatggggcc tctccccccg gatttgagcg cacgcgaagc ctcacgtctt gcgacgaagc
     4081 cgaggtattc gagaatttca aatatcttta gaaattggct aaccgtctgc cgatgtatca
     4141 aaatccggtc ttgagcagta tttcgagtat tacttttaac cgttttcgtc gcgtgatcaa
     4201 caataacgcc aaaaacttct ctagctggat caacgagagc ttcgtagata ccaagcttcg
     4261 gcgccacctc atccattagt ggccagaagt agtcggatgc catatcaagt agacattgcg
     4321 atacttcagg aattccgatt ttgtccttat tgtcgaatct attacaggcc aatataaacc
     4381 cacgtagatt gccgaccacg gcgcgtccaa gaaggttagc aaattgggag ggatctaatc
     4441 ggtcggaaaa agtagcgcgc tctgaaagtc ggggataccg agctttaact acatcaggaa
     4501 agaacccggg tgcagaagcg gtgtcggatc gaccaatatc gattacggtc gagtcattga
     4561 agacatcgaa acggacaccg aatttcgtta cgccgggata gatcgatgcc ttgcaggaag
     4621 tgaggctact cgacaaagta cgatacaagt cgaagaagac ctcaagcggc ttttctcgac
     4681 caatgtgtgc agcgtcatca aacaacagaa caatcctcat gttcagcgcc tgcgccaaag
     4741 cagtcagacc gaactgtagc gacacctgat ctgtcgcttc tgcgaagtcg tgctcaagct
     4801 ttcggtcgct gagttccttc tgaattgcct gggagagaag gatgcagaag tgaccgatat
     4861 actcgtttcc gtccgattgc agtaagggaa ggtaccgtag gctgacgtag acgggaaaca
     4921 cactcgttga caccctcatc cgtcgaaagg actcaatcaa gagtgcgctc tttccgctgc
     4981 ctcgaccgcc tcgcacgagg actggtccgt gagcgctcaa ctttcgaatt atgacttcgt
     5041 cgtgtaacga aaatcggtgg taattttcaa attggtttgg agggcattct tctgcggcga
     5101 ggatgaggcc agagtccaaa ttagtgtcgt tacaggtatt caaggtacga gccttctgat
     5161 tgccttgtag catctagccg cttcgtcggg agcgagggat gcgtatgcgg tgtgcacgaa
     5221 atgggagacc agcccgagat ttgccacaag caaaaatgtt tcccggattg aatttttaac
     5281 agtggcttcg tcaaatagta gcgcgctcgt gaaaaactcc caatgcactc cttttgacaa
     5341 agttgaatag agctggatac tcctgccctt ggtttcatct atgaatcgat ctggatcaat
     5401 attggccaca tcggctagca aaggatcaga ctcaaggcta gaaacgtagt caagcattct
     5461 atttattgcg ggtttccaga agatatgttc gaaatacttg gaaaacagcg cgcgactgat
     5521 tttgggaagg tcgtgatcga tactccacaa agcttgattg ggcttttcat caggtatcac
     5581 atcaccagac caagcaaatg ctgacttcgc acgcttccct ggttcatatt ccggctgcgc
     5641 ttggaactcg ctcaaataga gcatacggaa cgagtcgaga cgtcccacaa gtgcagcgca
     5701 gccattctcg aggagaacgc gtgagcaaaa ggggccgaag ctaagggctt cgtctgtgat
     5761 gtcctcggtc aaaaacgtgg ctgcctttgc ggagaaactt cgaagtgaac tcgcaagttg
     5821 gtcaagggca atttttccag ttccctccga gttgtcgccg catagtagaa gtgcaatttc
     5881 attcatttga tcgagagtcg gtacgaaact gatgcgcatg gggttggctg cacggataga
     5941 agctcattaa acctactatg catgaaaaag catgcagcaa gatgtctcga aggcatatgt
     6001 tctctctagt ctaacaggca ggcgatccga tgcaccattg gaaagcagtc gttcacggat
     6061 gcccgcaact ggcccaaacc gggtactccc aattttgatt cgactgaccg ctcccactgc
     6121 gttgctgtcg ttgccagcca tcttccaatc aaccatgcca cccatggttt accggttcga
     6181 aatcgggcct ggcggcaaca ttctttgggc cagccaggtg ggactggggc ttgcgggcct
     6241 atcagcctcc tgcgaggccg acgcatgcac ccccaggacc cggtttaacc agtgggccgc
     6301 attgcgcacg cgggtgggaa catctttgtc cagccgcagt acggggcagt gcacattctg
     6361 caattgcgtc aaataccagt cgtgctgtcg ctggcgaaat ccatcgtcgc gtcgcgtgcc
     6421 atcctgcaca aacgcaaaat ccggctcgca caggatcagc gcgtcgtacg gatgacgcgc
//
LOCUS       DAOSLL010000217         6437 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10902_length_6437_cov_4.663115, whole genome shotgun sequence.
ACCESSION   DAOSLL010000217 DAOSLL010000000
VERSION     DAOSLL010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6437)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6437)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6437
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10902_length_6437_cov_4.663115"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PLB25_10565"
     CDS             <1..918
                     /locus_tag="PLB25_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOE42055.1"
                     /translation="TTGSQQGLDLVAKVLIDAGSKILVEAPTYLGAMSAFVPMEPEVV
                     SVANAGSGLDLADLSAKLPDARFLYVLPNFQNPTGRTMSEQCRAELSALASQRGLPLI
                     EDNPYGDLWFDTPPPASLTARNPQGCIYLGSFSKVLAPGLRLGFLVAPKAVYPKLLQA
                     KQAADLHSPGFNQRMIAEVMKDGFLDHHVPTIRALYKAQRDAMLAALAQHFPNHKIQP
                     DQSLTWNTPAGGMFLWARLPAGMSAADLLPFAIDQGVAFVPGAPFYADLGDPRTLRLS
                     FVTPSVADIQRGVAALAKALQIYTATVSG"
     gene            1048..1716
                     /locus_tag="PLB25_10570"
     CDS             1048..1716
                     /locus_tag="PLB25_10570"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOE42056.1"
                     /translation="MGTLYLVRHGQASFGTDDYDQLSALGKRQSVRLGHYFARKDLHF
                     DAILSGTLRRHTQTLAGISEGLGVPLTAVQWPGLNEYDSRAIIGAIHPAPLEPASPPD
                     QYRHYFRLLRVGLTHWMLGKTQPVGMPDWRQFQQGVVEVLNYIRQTDAQQVLLVSSGG
                     PIACAIGHVLATPSDATVELNMRLRNSSVTEFAFTPKYHALQTFNTLPHLDALEFDAW
                     VTYA"
     gene            complement(1796..2305)
                     /gene="phaP"
                     /locus_tag="PLB25_10575"
     CDS             complement(1796..2305)
                     /gene="phaP"
                     /locus_tag="PLB25_10575"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HOE42057.1"
                     /translation="MITFDQISASSSANLNTVKGLSTKTYVGFGKMLELNLASVRDLM
                     NGIFTHIERLLAARDAQQFLALQTDQLEPLAARATSYGRHVYGIAVEASAEFAKTFEG
                     KAAEGQKVLGQVMQEIVKNAPPGSESAVAVIQKALASSQSAMDSAQAVTRKALVLTAN
                     ITAAVVAQD"
     gene            complement(2456..5989)
                     /locus_tag="PLB25_10580"
     CDS             complement(2456..5989)
                     /locus_tag="PLB25_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE42058.1"
                     /translation="MQKPLRSITELRQRAEAELRRKGPQVPDTLQDLSREQLQQLLYE
                     LSVFQLELELQSQELLQATSELESSRADYADLYNSAPVGYCTLDESGRLLQVNLACAR
                     LLGQDVADLTGKLLRHFVDPIDRSVFASFCSDLLQSGHTKAVEIRLTGHAAAPIWVLL
                     EASLTRQPDTHPLLRVIMVDVSARKLAQQAADSSHDTLKGILDTTLDGFWQLDRAGRL
                     LDVNAVYCQQSGYRRADLLGCLVDDFDAQQNPSCIAEQLKHLIRYGHMVFETRHRRQD
                     GSLWDVEVSATFDPQGGGLIYAFCRDVTARKQTARQLADSEERWKFAVEGSGEGVWDW
                     HIDTGHAVFSKQYKEMLGYAEEDIGNDASEWTSRVNPQDMPRVMQVLQDHLAAKTSAV
                     RVEFRMRCKDGSWRWMLGRGMVVARDAAGKPVRLVGTNSDIHEPKQVQQQEQLHRLVL
                     ELLSSDAPWEQVLLALVQGVGQAVPDVLCAILLLDEAGSRFVYSVAPGLPDSFTSAVL
                     RASADAGVGCSGLFACTGERQMVPDMAAHAGWQQVCAEAACAGLAACWLQPIRGTAGH
                     VLGSFVVYRRQTHCPDAAEIALVENCAHLAGIALERHRAAEKLRASEAHFRLLTEDVS
                     DVVWKLDVDFRVTYISPADEAMRGYRADEVIGSSMLEWLAPEGVAKIEKRLEAWADMG
                     QLPHGSVIDEVQQRCKDGRMIWTEVLARMEFDALGRISGFHGTTRDISRRRLAQAALQ
                     ESEDRFRTIVEHSPDPVAVFRAGRLIYANSAAVRLLRAPNVQVLMGVSMLELVHPDSK
                     LAVQVRASALQRPGDTTDLLEEKLLRLDGTTVHVEAQSTVMVFNAEPAIHVTVRDISV
                     RKQAEERLRLAAAVFNHTREGILVLSLAGIVIDLNDAVTRLTGYSRAEVLGHSPRERF
                     CAELHAPEVYEVMARELHEKGFWSGELWNRRKNGEVFASLQAISMVRDESGVEQSLVV
                     LFSDISALKVHEKELERMAHFDVLTGLSNRVLLADRMRQAMAQALRREQSIAVVYLDL
                     DGFKAVNDSRGHTVGDRLLMALANRMRQALREGDTLARVGGDEFVALLVDIDRTGCDT
                     MVARLVDAAAQPVLMDGFSVQVSASLGITFYPQPHDIDAELLLQQADRAMYQAKLAGK
                     NCYRYFQAEPARATNLMAGLAPIDCPGEVCD"
BASE COUNT         1335 a         2092 c         1766 g         1244 t
ORIGIN      
        1 accaccgggt cgcagcaggg gctggacctg gtggccaaag tgctgatcga cgcgggcagc
       61 aaaattctgg tcgaggcacc cacctacctc ggtgcgatgt cggcctttgt cccgatggag
      121 cccgaagtgg tcagcgtggc caatgctggc agcggtctgg atctggctga tttgagtgca
      181 aaactgcccg atgcccggtt tctctacgtg ttgcccaatt ttcagaaccc cactggccgc
      241 accatgtccg agcaatgccg cgccgaactc agtgcgctgg cttcccaacg cggcctgccg
      301 ctgattgaag acaaccccta cggcgacctg tggttcgata ccccgccacc cgcctcgctg
      361 acggcgcgca acccgcaggg ctgcatttac ctgggctcgt tctccaaagt gctggcacct
      421 ggcttgcgcc tgggttttct ggtcgccccc aaggcggttt accccaagct gctgcaagcc
      481 aagcaggccg ctgacctgca cagcccgggt ttcaaccaac gcatgattgc cgaagtgatg
      541 aaagatggct ttctggatca ccatgtgccg accatccgtg cgctttacaa agcgcagcgc
      601 gatgccatgc tggccgcgct ggcgcagcat ttccccaacc acaaaatcca gccggaccag
      661 tcactgacct ggaacacccc ggcgggcggc atgtttttgt gggcccgcct gcctgctggc
      721 atgagcgcgg ctgacctgct gccatttgcc attgaccagg gtgtggcttt tgtgccgggc
      781 gcgccttttt acgccgactt gggcgacccc cgcacgctgc ggctgtcgtt tgtcaccccc
      841 agcgtcgccg acatccaacg gggtgtcgca gcactggcca aagccctgca gatttacacg
      901 gcgacggtgt caggctgatg caaacgcagc gcaacataat ttgcccatga accgtcccag
      961 ccacctgaag gtctgtcttt gccacaccac ggcaagatca tccaagacgt gacaaacaaa
     1021 ccctctggta ttgcctgaat tgcccgcatg ggaactcttt acctcgttcg tcacggccag
     1081 gcctcgtttg gcacggacga ctatgaccag ctcagcgcgc tggggaagcg gcaaagtgtc
     1141 cgccttggtc actactttgc ccgcaaagac ctgcatttcg atgccatcct gagcggcacc
     1201 ctgaggcggc acacgcaaac gctcgcgggc atcagcgaag gtttgggcgt gccactaaca
     1261 gcggttcaat ggccgggtct gaacgaatac gacagccggg cgatcattgg cgcgattcac
     1321 ccggcaccgc tggagcccgc cagcccaccg gaccagtacc gccactactt tcgcctgctg
     1381 cgcgttggcc tgacgcattg gatgctgggc aaaacccagc cagtcggcat gcccgactgg
     1441 cggcaattcc agcagggggt ggtcgaggtg ctgaactaca tccggcaaac cgatgcgcaa
     1501 caagtgctgc tggtgtcaag cggcgggcca attgcctgcg ccatcggcca tgtgctggcg
     1561 acaccgtccg atgccacggt ggaactcaac atgcgtttgc gcaacagctc cgtcaccgag
     1621 ttcgccttca cgccgaaata ccacgcgttg caaaccttca acaccttgcc acatctggat
     1681 gcgctcgaat tcgacgcatg ggtcacctat gcctgagcgg catcatgcgc ccaacaaaaa
     1741 agcacccgaa ggtgcttttt tttgtcctga accctgagcg aatcagcttg tcagttcaat
     1801 cttgcgcgac caccgcagcg gtgatgttgg ccgtcagaac cagcgccttt ctggtgactg
     1861 cctgagccga atccatcgcg ctttggctgg aagcaagggc tttctggatc acagccacag
     1921 ccgactcgga acccggcggt gcgtttttga cgatctcctg catcacttga ccgaggacct
     1981 tctggccttc agcggctttg ccttcaaagg tcttggcgaa ctcggcgctg gcctccaccg
     2041 cgatgccata cacatgccgt ccatacgaag ttgcccgcgc ggccaggggc tcaagttggt
     2101 ctgtttgcaa cgcgaggaat tgctgcgcat cgcgggccgc cagcaggcgc tcgatatggg
     2161 taaaaatgcc attcatcaaa tcccgcactg aagccaggtt cagttcaagc atcttgccaa
     2221 agccgacata agtcttggta gacagcccct tgacagtgtt cagattggca ctgctggagg
     2281 cgctgatttg gtcaaaggtg atcatcatga tggcatcttt caaatattgg tttttatgct
     2341 cccacagaag cttcacagaa gctgtgctgc actgcaacat gatacaacta ttagggatta
     2401 cccgacattt tactggggtt ttgctgcagt gcagcaaata tttagaaacc aagctctaat
     2461 cgcacacttc accggggcag tcaattggcg cgagcccagc catcaggttc gttgcccgag
     2521 ccggttccgc ctgaaaataa cggtagcagt tcttgcccgc cagtttcgcc tggtacatcg
     2581 cgcggtcggc ctgctgcagc agcagttcgg cgtcaatgtc atgcggctgc gggtaaaaag
     2641 tgatccccag gctagccgac acctgcacgc tgaaaccatc catcagcacc ggttgcgctg
     2701 ccgcatcaac cagacgtgcc accatggtgt cgcagccagt ccggtcaatg tccaccagca
     2761 aggcaacaaa ttcgtcgcca ccaacccgag ccaaggtgtc accttcgcgc aacgcctgcc
     2821 tcatgcggtt ggccagcgcc atcaacagac ggtcaccaac cgtatgaccc cggctgtcgt
     2881 tcaccgcctt gaatccgtcg aggtccagat acaccaccgc aatcgattgc tcgcgccgta
     2941 gcgcctgcgc catcgcctga cgcattcggt ccgccagcaa cacccggttc gacaagccgg
     3001 tcagcacatc aaagtgcgcc attctctcca gctccttttc gtgcaccttc agggccgaaa
     3061 tgtctgaaaa cagcaccacc aggctctgtt cgacacccga ctcgtcgcgc accatgctga
     3121 tggcctgcaa agaggcaaac acttcaccat tcttgcgccg gttccagagt tcacctgacc
     3181 agaaaccctt ttcgtgcaac tcgcgtgcca tcacctcata gacctctggg gcatgcaact
     3241 cggcacaaaa ccgctcacgc gggctatgcc ccagcacctc ggcgcggctg tagccagtca
     3301 agcgagtcac cgcgtcattg agatcaatga caatgccggc cagcgacagt accaatatgc
     3361 cctcacgggt gtggttgaac acggcggctg ccaggcgcag acgttcctca gcctgcttgc
     3421 gcacgctgat gtcgcgcacc gtgacatgaa tcgccggctc tgcgttgaac accatcaccg
     3481 tgctttgggc ctcgacatgc accgtggtgc cgtccagccg gagcagcttt tcttccagca
     3541 gatcagtcgt gtcacctggt cgctgcagcg cactggcacg cacttgcacc gccaacttgc
     3601 tgtcgggatg caccagctcc agcatagaaa cgcccatcaa aacctgcaca ttaggggctc
     3661 tgagcaggcg cacagcggca ctgttggcat aaatcagccg accggcgcga aacaccgcaa
     3721 ccggatcggg cgagtgttcc actatggtgc gaaagcggtc ttcgctctct tgcaaggccg
     3781 cctgcgccag tcggcgacgg gaaatgtccc gggtggtgcc atgaaagcca gagatgcgcc
     3841 cgagcgcgtc aaactccatt ctggccagca cttcggtcca gatcattcgg ccgtccttgc
     3901 aacgctgttg cacctcgtca atcaccgagc catgcggcaa ctgccccatg tcggcccagg
     3961 cctccaggcg tttttcaatc tttgcgaccc cttctggggc cagccattcc agcatgctgc
     4021 taccaatcac ctcatcggca cgatagccgc gcatggcttc atctgccgga ctgatgtagg
     4081 tcacccgaaa gtccacatcg agcttccaca ccacgtctga cacatcttct gtcagcaacc
     4141 ggaaatgcgc ttcactggcg cgcagctttt cggccgcccg atgccgttca agagcaatgc
     4201 cagcaaggtg ggcacagttt tcaaccagcg cgatttcggc tgcatcaggg caatgagtct
     4261 ggcggcggta caccacaaag ctgcccagca cgtgcccggc agtaccgcgt atgggctgca
     4321 accaacaggc cgccagaccg gcacacgccg cctcggcaca gacctgttgc cagccggcat
     4381 gggctgccat gtcgggcacc atctggcgct cgccggtaca ggcaaacagg ccagagcagc
     4441 cgaccccggc atcagccgac gcacgcagca ccgcagaggt aaaagaatca ggcaggccgg
     4501 gagccacgct gtagacaaac cggctgcccg cttcatcgag cagcaagatg gcgcacaaca
     4561 cgtcagggac agcctgccca accccctgca ccagcgccag cagcacctgt tcccagggcg
     4621 catcagaact cagcaactcc agcaccaggc ggtgcaactg ttcctgctgc tgcacctgct
     4681 ttggttcgtg gatgtcactg ttggtaccaa ccagccgcac cggtttgcca gcagcatcac
     4741 gcgccaccac catgccgcgt cccaacatcc agcgccaact gccatctttg cagcgcatgc
     4801 gaaattcaac ccgcacggca ctggtcttgg cagccagatg gtcttgcaga acctgcatga
     4861 cccgcggcat gtcctgcggg ttcacccgac tggtccattc agaggcatcg ttgccgatgt
     4921 cttcctcggc atatccgagc atttccttgt attgctttga gaacaccgca tggccggtat
     4981 cgatgtgcca gtcccaaacc ccttcgcctg atccctctac tgcaaacttc cagcgctctt
     5041 cgctgtcagc aagttgccgg gcggtttgct tgcgcgccgt aacgtcgcgg caaaaagcgt
     5101 aaatcaggcc gccgccctgc gggtcaaagg tggcactcac ctcgacatcc caaagcgagc
     5161 cgtcctgacg acgatggcgg gtctcaaaaa ccatgtgacc gtaccggatc aggtgcttga
     5221 gttgctcagc gatgcaagac ggattctgct gagcatcaaa atcatccacc aggcagccca
     5281 gcagatcagc ccgccgatag cctgactgct ggcaataaac cgcattcaca tcgagcagcc
     5341 gaccggcgcg gtcgagttgc cagaagccgt caagcgtggt gtcaagaatg cctttgagcg
     5401 tatcgtggct gctgtcagcg gcctgctgcg cgagcttgcg cgcactgaca tccaccatga
     5461 tcacgcgcag cagcggatgg gtgtctggtt gccgtgtcaa cgaggcttcc agcaaaaccc
     5521 agatcggtgc agcagcatgg ccggtcaacc tgatctcgac cgccttggtg tggccgcttt
     5581 gcaacaagtc agagcagaat gaggcgaaca ccgaacggtc aatcgggtca acaaaatggc
     5641 gcagcagttt gccggtcaaa tccgccacat cctgccccaa caaccgcgca caggccaaat
     5701 tgacctgcaa tagccgcccg gattcgtcca gcgtgcaata accaaccggc gcgctgttgt
     5761 acaagtccgc gtagtcggcg cgtgacgact ccagttcact ggtggcttgc aatagctcct
     5821 ggctctgcag ttccagttcc aactgaaaaa cgctgagctc ataaagcaat tgttgcagtt
     5881 gctcacgcga caagtcctgc agcgtatccg gcacctgcgg cccctttcgg cgcagttctg
     5941 cctcggcgcg ctggcgcagc tcggttatgg aacgaagagg cttttgcatg gtggtggcac
     6001 ggtcgggcaa cgaatgaagc ttcaggacaa ccaagaaatg gcgcgccgca accgtggccc
     6061 ggagcacctt tgccaaaccg cagcattttc gggcttggac cctgaaagtc aactacccaa
     6121 acgcatcgtt tcagcgcaaa ttggttctta tcaaaaaatg gttggtccta gattcctcta
     6181 gattcctcat ttggacgcgc ccgagtgggc tgctggtggt gcggttgggc agggcgaaag
     6241 ccgcggctcc aaacctgccc gcgcaggttt ggacggcttg aagcaggcca gccttgtggc
     6301 atggctgcgc cctgcgtgtc gcagcaagct gcgaggggag acgacgacgc gggctcctga
     6361 ccgccaggag gagcaacgac cccaagcgtg ttgccagtgg ctcaaccggg tgcgtagcgc
     6421 tgccacccaa tgggtga
//
LOCUS       DAOSLL010000218         6432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10912_length_6432_cov_4.058021, whole genome shotgun sequence.
ACCESSION   DAOSLL010000218 DAOSLL010000000
VERSION     DAOSLL010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6432
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10912_length_6432_cov_4.058021"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(663..1367)
                     /locus_tag="PLB25_10585"
     CDS             complement(663..1367)
                     /locus_tag="PLB25_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42059.1"
                     /translation="MALPIIVPAWEFELQDRFQNTHALPSKRLPPRPIRILRPLNAAD
                     TETREPTERELLITRWCRVANVYSPTPFGRKATASIYNRLANDTESADCGVSLLATCE
                     WLLRKRFRREWALLDTAKRAQVLTLVKRFKTEAPIFGATELAADAAAWTLFAMYWCGA
                     SHPAALFKESLHPPTGLVRWISQFHIDQQRNQVREIQEQFAHYLGVTRGLCDVLVRRM
                     LKRTASVALLRELCDF"
     gene            complement(1373..2422)
                     /locus_tag="PLB25_10590"
     CDS             complement(1373..2422)
                     /locus_tag="PLB25_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE42060.1"
                     /translation="MTDSMQPTVAATRVKQTSPKKAAPVWTVPQPASLTLQNHPLVRR
                     HYRVATAAIEQFVDLVERCIRVLVPGALIHARPRMGKTHAIDYVALHLERHRPDILVL
                     RMSCEHHRSDFEGPFFSTLLSAAGVRHPQPKSITDKRFALMCRLSEQLQMRAGHIVVL
                     LCDEAQRLSKHALEWLRDVHDQLAHHGVRLLTFLVGQPQLMEQKAHYQLSGDEQIVAR
                     FMIEQQHFRGIGDAVDAATCLASYDLSRYPETTGKTFTEFFYPRACERGLLLEQSAAM
                     LWNTFVNAHASAQLAGPAEIPMDYFTRAVESVLLQGAQWDTQDLALGVAHWERAVKES
                     GYVAAQQTVSVMPGQ"
     gene            complement(2548..4464)
                     /locus_tag="PLB25_10595"
     CDS             complement(2548..4464)
                     /locus_tag="PLB25_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42061.1"
                     /translation="MIRRSIELLRIAEWPRLDEQALEEPARQIYQSRCAALEAYAQGE
                     PVASIEAAHGIDRSTLFRMVKRAQRAHADGSIWGYRALEPHVNVSGYERSRPPRALVH
                     TKAGNAGAFTQLLQRHPALEAHLRRELSAQRVELKGVGEAARLFNFKPALQRFQQACR
                     QQGLLPSDYPFNQQDKGVRSLARTLRAWLLDRFDDAAHAAGARFKPSSALRQLPERGV
                     DEAFDTVEFDAHKMDLRLKVVDVDPLGIERTYETERVWLLAIIDVATRCILGYTLCLH
                     RECSRHEVIATLKHALTPAEIPKITLPGLSLSGSGGLVSQALPITQYACWRQIRLDNA
                     RAHLATTSLDVLCEALGCVADFGPAYEPDDRPFIERFFGTLTNTLSRRLPGGLPSKQA
                     TTSKRSREAAKDLSLVVTVQELEELLDATVWNYHGTPHSSLGGLTPLERMSQQLDGVG
                     LPPVRLRRVPEALRNRLELLHDPTFCLVHGNVGRGERPYISFMHVRYTSEQLARSSNL
                     IGKQLRVYFDAKDLRTLRAFTEEGQPLHDLLAGGPWRHEAHSLRLRQEVFKAKRNKQL
                     VFMAGESPIDAFVKLRRAKAPKSRRAASDLARVQRERRDAPVTAPHPVEPSAQLATGV
                     VKGKKLRIARGFAR"
     gene            complement(4461..5123)
                     /locus_tag="PLB25_10600"
     CDS             complement(4461..5123)
                     /locus_tag="PLB25_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011295991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42062.1"
                     /translation="MTKSMPYTRAVPFSRPAGRRRIEVFSPKIGRRLCLGGYDAYRTW
                     LVIEANPLIVTFCERPTYVDGPKSPVIDFWVQLRGVPAGEFWMVEGLPRPSSKLAITP
                     PERHSHLHELPVRYINRADLIGWAEPIGNWARIIPYLVSHRRYRDTLLEQQIMAFLNE
                     DESVDAVLAHFPQRDATTAQAALFQLLAEGRVTSPDLAIEPMDGCTRFHRLPVVLAST
                     PS"
     gene            6026..6280
                     /locus_tag="PLB25_10605"
     CDS             6026..6280
                     /locus_tag="PLB25_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42063.1"
                     /translation="MRGDNYLKFIWKPFMTRFLRLSSLALALIHSVGPSFAQTGAQGT
                     GAWAPGQTMPQTRIIQGRNSNYALPVGWRVGEKGNHALVL"
BASE COUNT         1306 a         1938 c         1872 g         1316 t
ORIGIN      
        1 gaacggccgg tttggatgag gtattttgcc aagttggctg accgctgagg gtctgttgag
       61 gtcagccgac caaaatttgc cccgttttca ctcgacgggc tccagtagac ccattgctgc
      121 ccttcgccga cgcctaaaga aactcaaagg atgtgcgaga gccgacgttc gctttcgccg
      181 gaagctgcca gtcgctggcc gtgccagaat ctcgtaagcg gtctttcgcg gacgaatttc
      241 agtgtgttgg actggccgtc ggcagcgcag cgattgcagt agctgagccg gcggcggtca
      301 atttcagcac tgggccaagt gctgacgctc acgaacggca gctaactgga tgtcaaaatt
      361 tcctcagtga aattaccaga aacctgactt gagccagcga ctcaaacgtg cccactgctg
      421 tccacggcga aggtcaggac cggccatttg ttcccttggt tcgatgggcc aaagctgccc
      481 ctcactattg caccaaaaaa ccaggggatc cctttctggg ggaaccatcc gcccttacca
      541 ccaagtattc gagggcgcgg taccgaacta accgcatcac gtcagcaacg ggaacgctac
      601 cgcgcctgct ggcgtgaagt gtagtaatac tgacaccaac aagtagtaat gttgccacta
      661 aatcagaaat cgcacagttc ccgcagcaac gccacgctgg cagttcgctt cagcattctc
      721 cgaaccagaa catcacagag acctctggtt acacccagat agtgggcaaa ctgctcctga
      781 atctcacgca cctggttcct ctgctgatcg atgtgaaact ggctgatcca tcgcaccagc
      841 ccggtcggcg gatgcaagga ctctttgaac aacgcagccg gatgacttgc tccacaccaa
      901 tacatcgcaa acaaggtcca cgcggccgcg tcggccgcca gctcagttgc tccaaagatg
      961 ggggcctctg tcttgaaccg cttcaccaat gtgagcacct gggcgcgttt cgcggtgtcc
     1021 agcagcgccc actcccgacg aaaccgtttg cgcagcagcc attcgcaagt ggccaacaag
     1081 ctgaccccac agtcggccga ctccgtgtca ttcgctaagc ggttgtaaat cgaggccgtc
     1141 gccttgcgcc caaagggcgt tggtgagtac acgttggcga cccggcacca tcgcgtgatc
     1201 agcaactccc gctcggtagg ttcacgcgtc tcagtgtctg cggcgtttag cggccgcagt
     1261 attctgattg gccgaggcgg cagacgtttt gatggcaagg catgggtatt ttggaacctg
     1321 tcctgcagct caaactccca cgccggcacg atgattggca gtgccattgc aatcactgtc
     1381 caggcatgac cgacacggtc tgctgggccg caacataacc ggactccttg accgcgcgtt
     1441 cccagtgcgc cactcccaac gccaggtcct gcgtgtccca ctgcgccccc tgcagcaata
     1501 cactttccac cgcgcgcgtg aagtagtcca tgggaatttc cgccggaccg gccaactgcg
     1561 cagacgcgtg cgcgttgaca aacgtgttcc ataacatggc cgccgattgc tcaagcaaca
     1621 agccgcgctc gcacgcccgc gggtagaaga actcggtgaa cgtcttgcca gtggtctcgg
     1681 ggtagcgact caggtcatag ctggccaggc aggtggcggc atccaccgca tcgccaattc
     1741 cccgaaagtg ttgttgctcg atcatgaacc gcgccacaat ctgctcgtca ccgctgagct
     1801 ggtaatgcgc cttctgttcc atgagttggg gttgccccac gagaaacgtt aagaggcgaa
     1861 caccatggtg tgccagttgg tcatgaacat cacgcagcca ttccagggca tgttttgaca
     1921 aacgctgggc ctcgtcacac agcaggacca cgatgtgtcc cgcacgcatc tgtagttgtt
     1981 cactcaacct gcacatgagc gcaaagcgtt tatctgtaat cgactttggc tgtgggtggc
     2041 gcacaccggc cgcgctcagc aaggtactga agaatggccc ttcaaaatca ctgcgatggt
     2101 gctcgcagga catacgcagc accaggatgt cgggacggtg gcgttcgagg tgcagcgcga
     2161 catagtcaat ggcgtgggtc ttgcccatgc gcgggcgggc atggatcagg gcgcccggga
     2221 ccaacacacg aatacagcgc tcgaccaggt caacaaactg ttcaatggcg gcggtcgcga
     2281 ctcggtagtg ccgacgcacc aggggatggt tttgcagtgt caacgaggcc ggttgcggca
     2341 cggtccagac gggcgcggcc tttttgggcg atgtttgctt gacgcgcgtt gccgccactg
     2401 tcggttgcat ggaatcggtc atgaatgcct ttcacgcctt ctcggcgctt gcggccggca
     2461 tgaaggcccc ggcccgacct catggcgctc aattgcgcca atgacccgtc ggtgccgccc
     2521 gggcggcccg caagctgtat ttgcttttta ccgcgcaaaa ccgcgcgcaa tgcgcagctt
     2581 cttgcctttg accactccgg tggccaactg agccgatggc tccaccggat ggggcgcggt
     2641 tacaggagca tcacggcgct cccgctgcac gcgcgccaga tcgctggccg ccctgcgcga
     2701 tttgggtgcc ttggcccggc gcagtttgac gaatgcatca atcgggcttt cgccggccat
     2761 gaacaccagt tgcttgttcc gcttggcctt gaacacctcc tggcgcagcc gcaaggaatg
     2821 cgcttcatgg cgccaggggc cgccggccaa caaatcatgc aatggctgac cctcctcggt
     2881 gaatgcccga agcgtgcgca aatccttcgc atcaaaatac acccgcagtt gctttccaat
     2941 caagttcgaa ctgcgcgcca gttgttcgct ggtgtagcgc acatgcatga aggagatgta
     3001 aggccgctcc ccgcggccca cattgccatg caccaggcag aacgtcgggt cgtgcagcag
     3061 ttccaagcgg ttacgcaggg cctctggcac gcggcgcagg cgaaccggtg gcagccctac
     3121 cccatcgagt tgttggctca tgcgctccaa aggcgtcagg ccccccaggc tggagtgggg
     3181 tgtgccgtgg tagttccata cggtggcatc caacaactct tcgagctctt ggaccgtgac
     3241 aaccaggctc agatctttgg cagcttcgcg cgagcgtttg gaggttgtcg cctgtttgct
     3301 gggcaaaccc ccaggcaggc gccgcgacag cgtattggtc agtgttccaa agaagcgctc
     3361 aatgaacggc cgatcatcgg gttcatacgc ggggccgaag tcagcgacgc agcccaaggc
     3421 ctcgcacaag acgtccaggc tggtcgtggc caggtgggcg cgggcattgt ccaagcggat
     3481 ctgacgccaa caggcgtact gggtaatcgg cagggcctgg gacaccaggc cgccagaacc
     3541 tgacagcgac agacccggca gcgtgatctt ggggatctct gcaggcgtca atgcgtgctt
     3601 caaggttgcg atcacctcat gccgactgca ttcacggtgc aggcacaggg tgtatccgag
     3661 tatgcagcgg gttgccacat caatgatggc cagcaaccag acccgctccg tctcataggt
     3721 gcgctcgatg cccagcgggt cgacgtccac caccttgagt cgcagatcca tcttgtgcgc
     3781 gtcaaattcg accgtgtcaa aggcttcatc gacaccgcgc tcgggtagtt ggcgcagggc
     3841 ggacgagggc ttgaagcggg cccctgccgc atgcgccgcg tcgtcgaatc ggtcaagcag
     3901 ccaggcgcgc agggtccgcg ccaggctgcg cacgcccttg tcctgctggt tgaacgggta
     3961 gtccgaaggc agtagcccct gctgacggca ggcttgctgg aagcgttgta gcgccggttt
     4021 gaaattgaac aggcgtgccg cctcccctac tcctttgagc tccacgcgct gggcgctcag
     4081 ttcacggcgc aaatgcgcct ccagcgcggg atggcgttgc aatagctgag tgaaggcacc
     4141 ggcattgccg gccttggtgt gaaccagcgc acgcggtggt cggctgcgtt cgtacccgct
     4201 gacattgaca tggggctcca aggctcggta tccccagatg ctgccgtcgg catgtgcgcg
     4261 ttgcgcgcgc ttgaccatgc gaaacagggt tgagcggtca atcccatgtg cagcttctat
     4321 gcttgcgacc ggctcccctt gggcgtaggc ttccagggcc gcgcagcgtg attgatagat
     4381 ttgtcgagca ggctcttcca aggcctgctc gtcgagtctg ggccattcgg caatgcgcag
     4441 caattcaatg gagcggcgta tcatgatggt gtgctcgcca ggaccactgg caatctgtga
     4501 aatcgggtgc agccatccat gggctcgatg gccaggtccg ggctggttac tcgcccctcg
     4561 gccaacaatt gaaacagtgc tgcctgcgcg gtggtcgcat cgcgctgcgg gaaatgtgcc
     4621 aacaccgcgt ccaccgattc gtcttcgttc agaaaggcca tgatctgctg ttcaagcaag
     4681 gtatcgcggt agcgtctgtg gctgaccaag taagggatga tgcgcgccca gttgccaata
     4741 ggttcggccc agccaatcag gtcggctcgg ttgatgtagc gcacgggaag ttcgtgcaga
     4801 tgggaatgac gttctggtgg cgtaatagca agcttcgatg atggtcgcgg cagtccttct
     4861 accatccaga attcaccggc cggcacccca cgcaattgca cccagaaatc gatcacggga
     4921 ctttttggtc cgtcgacgta ggttgggcgt tcgcagaagg tgacgataag tggatttgct
     4981 tcgatgacca accatgtcct gtacgcgtca tagccgccaa ggcacaggcg tcgccctatc
     5041 ttgggactga agacttcaat gcgtcggcgc cccgccggac gggaaaacgg cactgcgcgg
     5101 gtgtacggca tcgacttggt catgaccttg cctcattgct tctgtacggg gtgcgtcttc
     5161 actctgcgac gcaagtggca atattactac gccaagtcag ttagtggcaa cattactact
     5221 tcaaaacaag tagtaatagt gccactcaaa tttcacgcgg gtgcgccaaa ccattgattt
     5281 atatggaaat ccgagaatct gcgtttgtgt cagtgttact acacttcacg gcatcgatgg
     5341 tgtggttgta caggccactg cggtcaccat cacaaacagt gcgagttcca acctgtacaa
     5401 atctgttacg gatagacgtg agcttgattt catgtcctgc attttctccc ttcatggctt
     5461 caagcagaga ggcgccgtta tcctagacaa agttcaactt cgccaccgcg accatgcccc
     5521 ttcaaatgcc tgctcccagc ggtgacaaac tcaaggcagc acggatcaag gcgggcttga
     5581 gtcaggtcga agctgcagcc ttaatggggt taccccgcgc agtccggcag tagggaagga
     5641 caacagtccc ggacctggca ggcgcttgag agttcgagtg atccgcgcaa tatgtctggc
     5701 cccatatttg caatgttcca gttgcgcacc aacagccact gcgagttttc attgatcaag
     5761 aaggtgccct ccactgagct ggatgctcct tgacccattt ggtgcataca actagccgaa
     5821 caggttgtcc gtatcctcct gagacagcct tgcgtcacca tcatcgacga tctcaggtag
     5881 gacctcagtg aaagtggcga tttcaacaac tcgttccagt tggcgtggat caaccatcag
     5941 gatacgtaat tccgccctgc ccctaggcga ttgcgcttga ttggggccaa ggcaactccc
     6001 gccgcacaca atcgaggaga acatcatgcg cggggacaac tacctgaagt tcatatggaa
     6061 gccattcatg acccgattcc tgcgcctttc gtcgctcgcc ctcgcgttga tccactctgt
     6121 cggtccgagc ttcgcccaga cgggggccca gggtacaggt gcttgggctc ccggtcagac
     6181 aatgccccag actcggatca tccaagggag gaactccaac tatgcgctgc cggtcggctg
     6241 gcgtgtgggc gagaaaggca atcacgcgct ggtgctctag gccaatgatt tgtcggcagg
     6301 cgtgatcgtg tacggcgtca gcggtcttgg ccaaaacctg acagcgccgc agttcgccta
     6361 cagcgttatc accaacaacc tgcggctggt gcccgatgtc cggatctcaa acccgacacc
     6421 gatccagcct at
//
LOCUS       DAOSLL010000219         6404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10973_length_6404_cov_4.414238, whole genome shotgun sequence.
ACCESSION   DAOSLL010000219 DAOSLL010000000
VERSION     DAOSLL010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10973_length_6404_cov_4.414238"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            588..1145
                     /locus_tag="PLB25_10610"
     CDS             588..1145
                     /locus_tag="PLB25_10610"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOE42064.1"
                     /translation="MNGLFKALPQSALIPLDGSSGGSVGKLSTEQATQLSASLIAGDM
                     VSAQAVVLITKSLFGVKFSLSGMTDWLKNNDFSFKKSQPAPAKADPIEQLASIVKYRA
                     LKDNLPEGDVLLFLDAAHPTMATKLGYGWSPKGERKIVATTAGKARVNVIGSLEPATM
                     KLIATFPETVNSETLAEHFARLRRI"
     gene            complement(1234..1995)
                     /locus_tag="PLB25_10615"
     CDS             complement(1234..1995)
                     /locus_tag="PLB25_10615"
                     /inference="COORDINATES: protein motif:HMM:NF022885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2894 domain-containing protein"
                     /protein_id="HOE42065.1"
                     /translation="MNELGHCGISNSLVSQPVVASELIDSLRLEGADKFDAVGLHFIA
                     VLAHRIDAHQGSVKRILDGKLAKALMAFKERFEQAQCGARDSIDQITPQFPLAVAELN
                     GLLKAGDFSGVKRVIASLKKSDHCVTLGDLARDLAQHSLENVTGSFVGNGAQQSELNT
                     TEYFRNTWSKLSSDRRVTQALAQAPQNPGPINSHMLVLRSLALMRDISPDYLNQFTSY
                     VDTLLGLDQFDKESKVNAKKTIDRKGRKKVKSRQG"
     gene            complement(1992..2630)
                     /locus_tag="PLB25_10620"
     CDS             complement(1992..2630)
                     /locus_tag="PLB25_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006224328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOE42066.1"
                     /translation="MDDSDGVEQAAPVWAVFGDLMSGLLGAFVLILVGVLGVQLETAT
                     HLQAEIQKRQVEEQRRMTLEKALAIPLAAGRITLNNGRIGISGRVLFSLNSDQLQPDG
                     RQLLNSLVTPLRTYLTSRDELLMVRGFTDNRSIREGNHRFADNWELSAQRALTVTRTL
                     IDEGMPSSMVFAAAFGAEQPVTPNSDETARAQNRRVEMAPVPKTTNSKPGTP"
     gene            complement(2630..4798)
                     /locus_tag="PLB25_10625"
     CDS             complement(2630..4798)
                     /locus_tag="PLB25_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF802 domain-containing protein"
                     /protein_id="HOE42067.1"
                     /translation="MNRHFFTVAFLLGAIALVWVGAGFIGSNILALTMTAIIGAVYVF
                     GMLELRHFRQASTTLTTALTKVPDQLTNLADWLDEVHPSLQNAVRLRIEGERIGLPGP
                     ALTPYLVGLLVMLGMLGTFLGMVVTLNGAASSLDGTTDLQAMRFALAAPIKGLGLAFG
                     TSVAGVASSAMLGLMAALSRHERMSAAQLLDTKIATRLLGFSLRHQRQEAYKALQLQS
                     QALPAVVDKLQTMMEQMQGMSQQLNERLLSNQETSHNEVKVIYSDLASSVAKSLSDSL
                     AQSTQAAGQSIKPIVEAAMAGLAQEARSLHQRVIDTTQMQLDGLSQRFNATAVAVTQT
                     WTASLANHERTSASMVSGLGQSLHSFTETFEQHCASLLASVNEANSNSLANQTSADQQ
                     RQQAWMQSLASMATTLQRQWHHTSEQTLSQQQQICTTLSNTAQEITEHAHANVSHTLG
                     EITRLVTCSENLISSRIASEADSIKQNSERMDQLARILQAEFGALRDDEAQRGHAAVA
                     RLGQLQDAVASHLTTLGTALEGPITRLIETASAAPRAAADVIGQLRLEMSNSILRDNE
                     LLQERSHIMETLNSLLNAIRHAADQQRSDIDSLVVTSAAALSDAGSQFSEKVDFETTK
                     LSEIAAHVTSSAVEVSSLSESFGFAVHSFNAANEKMIESLQRIEAAMNKSTTRSDEQL
                     AYYVAQAREIIDLSTMSQRQVFEELHQLSNKQARCAQEVN"
     gene            complement(4795..5631)
                     /locus_tag="PLB25_10630"
     CDS             complement(4795..5631)
                     /locus_tag="PLB25_10630"
                     /inference="COORDINATES: protein motif:HMM:NF023256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3348 domain-containing protein"
                     /protein_id="HOE42068.1"
                     /translation="MSKRGELIVAHVQFFLFQFLPYMTRAQTRTNFHSSKLIRVLSDM
                     AIVDAVEPGIAFADKLGLWLDLNDAIRLHAVHSVSPPAPVRQSAAESISGGGLGEELA
                     RIRAILVNSITKSGSPKRGRTKIEIPSNKHDAPIEVASAYEPYRRYYLAHQHEMDLSI
                     RPLRVNVREVLARASPALRQLAALDAALDATLCDRERKLLSTVPSLLERRFEQLLKTH
                     QQKLVDAQQEDNPTLWMKPGAWLACFCNDMRSALLAELEVRLQPTVGLIEAFNNDTTR
                     HR"
     gene            complement(6145..6306)
                     /locus_tag="PLB25_10635"
     CDS             complement(6145..6306)
                     /locus_tag="PLB25_10635"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOE42069.1"
                     /translation="MIRHSDAVNIASLFYLVPPCTALVAWLAFGESFSYMAILGMVPA
                     VWGVYLARK"
BASE COUNT         1502 a         1698 c         1656 g         1548 t
ORIGIN      
        1 agggtcgtta tgcacatctc tgcataagtg cccaaagccg acatccactg gaaccgcatg
       61 aagttcggcg gaaattggaa agctgacttg cgattttgtc agaaccagcc gttcggaact
      121 ggtgccagaa acttcggaag cagtcaccca gcgacgaaaa cccatttttt cgtttttagg
      181 tcagctgagc agcggttgcg ggtatttgcg aatggcagca ttctggaact ccaatgtaaa
      241 gccagtcaga agtcgttggt aaacctcaag tttgccgcgt ctggttgctc aagtatcatt
      301 tgtgcaatcg gccacggaaa actcacatcc ctgagtatgt tgtagccaat cgctgagtgt
      361 tctcgaacca agtttaattc agccgtaggc agttttcttg gtttggatag tatctcgcta
      421 ggaatgccaa ttttccccaa atcgtgaaca gtggcagcga gatgaagtcc gtgaacgcgg
      481 tcatctgcta aacccaattc agtgccaatc tttgagcaaa tggctgcaac tcttcgctgg
      541 tgtcctgcgg tataagagtc acgctcttca ctggtggctg aaattacttg aatggtctgt
      601 tcaaggctct ccctcaatcg gctctgatac cgctggatgg atcaagcgga ggttcagtcg
      661 gtaaattgag caccgagcag gccactcagt taagtgccag cttgattgcc ggtgacatgg
      721 tcagcgcaca agctgtcgtc ctgataacca agagtctttt tggtgtcaag ttttccctct
      781 cggggatgac tgactggctc aagaacaatg acttttcttt caaaaagagc cagcctgccc
      841 cagccaaggc cgaccccata gagcaactcg catccatcgt taaataccgc gcgctcaaag
      901 acaacctgcc agaaggagat gtgttgcttt tccttgatgc cgcgcatccc accatggcca
      961 ccaaactggg ctatggttgg tcacctaaag gcgagaggaa aatcgtggcg acaacggctg
     1021 gcaaagcgcg tgtcaatgtc atcggctctt tggagcctgc aaccatgaag ctgatcgcaa
     1081 ccttccctga aacagtcaat tcagagacct tggccgagca cttcgctcgg cttcgcagaa
     1141 tctagccctg tggctcgggc acgagagtcc gaccaccacg caacacggaa gtgacctgac
     1201 tttgattgag tgatcaattt tgcaacgcga ggatcaacct tgacgacttt tgaccttctt
     1261 tctgccttta cgatcaatgg tcttttttgc attgactttg ctttctttgt caaattgatc
     1321 aagaccaagc aaggtatcaa cgtaggacgt aaactgattg aggtaatcag gcgagatatc
     1381 gcgcatcaat gccaatgagc gcagcaccag catgtgagaa ttgattggcc cggggttctg
     1441 tggtgcttga gcaagcgcct gtgtcaccct tctatcactg ctgagttttg accaggtatt
     1501 tctaaaatac tcggtggtat tgagttcgga ttgctgtgcg ccatttccaa caaaactgcc
     1561 tgtaacattt tctaaagaat gttgcgcaag atcccgggca agatccccca aagtcacgca
     1621 atggtcactt ttttttaaac tggcgatgac tcgcttcacg ccgctgaaat ctccggcttt
     1681 gagcagtcca ttaagttcag caacggcaag cgggaattgt ggagtgattt ggtcgatact
     1741 gtctcgtgca ccgcattgtg cctgctcgaa ccgttctttg aaagccatta gcgctttggc
     1801 gagtttgccg tcaagaatgc gtttcacact tccttgatgc gcatcgatgc gatgggccaa
     1861 cacagctatg aaatgcagac caacggcgtc aaacttatcc gcaccttcca ggcgcaaaga
     1921 atcgatcaac tcgctggcaa cgacgggttg actcaccagc gaattcgaga tcccgcagtg
     1981 gccgagttcg ttcatggcgt gccaggtttg gaattggtgg tttttggtac aggtgccatt
     2041 tcaacgcgcc gattctgagc gcgcgccgtc tcgtcactgt ttggagttac cggttgctcg
     2101 gcaccaaagg cagcggcaaa taccatcgac gacggcatac cttcatcgat caaagtacgt
     2161 gtcacggtca aagccctttg ggccgatagc tcccagttgt cagcgaaacg atgattacct
     2221 tcgcgaatcg accgattgtc ggtaaaaccc ctcaccatca gcagttcatc acgcgatgtc
     2281 aagtaagtcc gcagcggcgt caccagacta tttaacagtt gccgaccatc tggctgcaac
     2341 tggtcggaat tcaacgagaa caatacccga ccgctgatac caatgcgccc gttgttcaag
     2401 gtgatccgcc ccgctgccag gggaatggcc aaagcttttt caagggtcat gcgccgctgc
     2461 tcttcaactt gacgcttttg gatctctgct tgcaagtggg ttgctgtttc cagttgaaca
     2521 cccaggacac ccaccagtat cagcacaaac gcccctaaca aacccgacat caggtcgcca
     2581 aaaacagccc aaaccggggc cgcttgctca actccatcac tgtcgtccat cagttcacct
     2641 cttgggcgca gcgtgcctgc ttgttggaaa gttgatgcag ctcttcaaaa acctgtcttt
     2701 gcgacatggt gctcagatca atgatttcac gtgcctgggc aacgtagtag gcaagctgct
     2761 cgtcacttct tgtcgtggac ttgttcatcg cggcttcaat gcgctgcaga ctttcaatca
     2821 tcttttcatt ggccgcattg aacgagtgca ccgcaaaacc gaaggattcg ctcaaactcg
     2881 atacctcgac cgcactgctt gtgacatgcg ctgcaatctc ggacagcttg gtggtctcaa
     2941 agtcaacttt ctctgaaaac tgactgccag catcgctgag cgccgccgcc gaggtcacca
     3001 ccagggagtc aatgtccgag cgctgttgat ctgcggcatg tctgatcgca ttgagcagtg
     3061 aattgagcgt ctccatgatg tgactacgct cttgcagcag ttcgttatca cgcaaaatac
     3121 tattggacat ttccagacgc aattgtccaa tgacatcagc agcggctcgg ggtgctgctg
     3181 atgcggtttc tatcagccgg gtgatcggac cttccagcgc tgtgcccagc gttgtcaggt
     3241 ggctggccac ggcatcctgc aattgaccca gccgtgcaac ggcggcatga ccacgctgcg
     3301 cctcgtcatc tctgagcgcg ccgaattcgg cctgcaatat acgggcaagc tgatccatgc
     3361 gttcactgtt ttgcttgatt gaatcggctt cggacgcaat tctggaactg atcaagttct
     3421 ccgaacaagt cactagccga gtgatttcac ccagagtgtg gctgacgtta gcatgcgcgt
     3481 gttccgtgat ctcctgtgcc gtattggaaa gcgtcgtgca gatttgctgc tgctgcgaga
     3541 gcgtttgctc acttgtgtga tgccattgac gttggagggt tgtagccatg gatgccaaag
     3601 actgcatcca tgcttgttgc ctttgctgat cagccgaggt ctgattggcc agcgagttgg
     3661 agttggcctc gttgaccgat gccaagagag atgcgcaatg ctgctcaaat gtttcggtga
     3721 acgagtgcaa tgactgacca aggccactga ccatgcttgc gcttgtccgc tcgtggttgg
     3781 ccagtgatgc cgtccatgtc tgtgtgactg cgacagcggt agcgttgaat cgttgagaaa
     3841 gcccatcaag ttgcatctgg gtggtatcga ttacgcgttg gtgcagcgac ctcgcttcct
     3901 gggcaagacc tgccatggct gcttcaacta taggtttgat actctggcca gccgcctgtg
     3961 tgctttgggc caggctgtcg ctgagtgatt tggccaccga tgaggccaga tcgctgtaga
     4021 tgactttgac ttcattgtga gaagtttcct gattgctgag caatcgttcg ttgagctgct
     4081 ggctcatgcc ttgcatttgc tccatcatgg tctgcaattt gtcaacaact gcaggtaagg
     4141 cttgggattg aagctgcagc gccttatagg cttcctgacg ctgatgcctg agtgaaaagc
     4201 caagcaatct tgtggcaatt ttggtgtcaa gcaactgcgc tgcagacatt ctttcgtggc
     4261 ggctgagcgc ggccatcagc ccaagcatcg ctgaggaggc aaccccggcc acagaggtgc
     4321 caaacgccag tcccagtccc ttgattggag cggcaagcgc aaagcgcatg gcctgaagat
     4381 cggtcgtacc atcgagcgaa gatgcggcac cattgagggt gacaaccatc ccaagaaacg
     4441 tgcccaacat acccaacatc accaatagtc ccaccagata gggagtcaat gcggggccag
     4501 gcaagccgat acgttcacct tcgatgcgca ggcgcacagc gttctgtagt gatggatgca
     4561 cctcgtcaag ccaatcagca agattcgtga gttggtcagg aaccttcgtc agcgccgttg
     4621 tcaaggttgt gcttgcctgt cgaaaatgac gcagttcgag cataccaaac acatagaccg
     4681 caccaatgat tgctgtcatc gtcagcgcaa gaatattgga gccaataaaa ccggcaccca
     4741 cccaaaccag cgcgattgcg cctaatagaa aggcgacagt gaaaaaatgt ctattcatcg
     4801 gtgtcttgtt gtgtcgttgt tgaacgcttc aattaaccca accgtcggtt gcaagcgaac
     4861 ctccaattcc gcgagcagtg ccgaccgcat atcattgcaa aagcatgcaa gccaagctcc
     4921 aggtttcatc caaagggtgg ggttgtcttc ttgctgagca tcaacaagct tctgctgatg
     4981 tgtcttgagt aactgctcaa atcgccgctc caagagcgaa ggcaccgttg aaagcaattt
     5041 gcgctcccga tcacacagtg ttgcgtcgag tgcggcatcc agggctgcaa gctgtcgcag
     5101 ggcgggtgat gccctggcaa gtacttcacg cacattgacc cgaagtgggc gaatactcaa
     5161 gtccatttca tgttgatgcg ccaaataata gcgacgatat ggctcatacg cagaggcaac
     5221 ttcaatgggt gcgtcgtgct tgttcgatgg gatttcaatt tttgttctgc ctctcttggg
     5281 cgagccgctt ttcgtgatcg aattcaccaa aatcgcccgt atccgcgcca attcttcgcc
     5341 taagccgcca cctgaaattg attcagccgc agattgccga acaggtgctg gcgggcttac
     5401 gctgtgcaca gcatgcagtc tgatggcatc attgaggtca agccacagtc ccagcttgtc
     5461 tgcgaacgcg atgcccggct cgacagcatc cacaatagcc atgtcggaga gaacgcgaat
     5521 gagtttggaa ctgtggaaat tggtacgcgt ttgtgctcgc gtcatatatg ggagaaattg
     5581 aaataggaaa aactggacgt gagcgacgat caattcaccc cgtttactca catgcaggtt
     5641 cgtttttgac aggttcgaag tacctgtaca accattgttg gatcgggaga gaaaaagctg
     5701 cttatgcgtc cagctcttgg gttacatccg atagcaatcg gaccggggat ggttttggcg
     5761 ctgttgaatc actcgatatc aagcgcagca tcatgcgata ctcaaacaac gcgtctcatt
     5821 ttactgtcac aaacagcgca tgccttttaa atggagctga gtcggtacca cagccatttt
     5881 ggaagatcga ctttcatcgg agcgaactac ggcgcagcta aacacttgct ctctgaaatt
     5941 caggattaca ggtgtcggcg gcagacttgg gggttaccca ggcatcgccc gttcccgatt
     6001 tctctggtag cccgccagcg cactggtgca aaaatcaacg tagccttttc tcatcgcggt
     6061 aaccagcttg atacgttatc tccgatgttg caggattttt ctcaaacctc ggacaagtcg
     6121 ttgcgtcgca tggaattggg ggcatcattt gcgcgcaagg taaacccccc agacggccgg
     6181 taccatgccc aaaatggcca tgtaagaaaa tgattcccca aatgcaagcc aggcaaccag
     6241 ggcagtgcag ggtggtacca ggtaaaacaa gctggcaatg ttgaccgcgt cgctgtggcg
     6301 gatcaatcag ttgagcaggc tgatggcccc aacggaaagc accaacacca gccagctcag
     6361 ggcaaatcag aactcacccg tccagtcaac acgaaaactc tctg
//
LOCUS       DAOSLL010000220         6404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_10975_length_6404_cov_4.014018, whole genome shotgun sequence.
ACCESSION   DAOSLL010000220 DAOSLL010000000
VERSION     DAOSLL010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6404
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10975_length_6404_cov_4.014018"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            44..859
                     /locus_tag="PLB25_10640"
     CDS             44..859
                     /locus_tag="PLB25_10640"
                     /inference="COORDINATES: protein motif:HMM:NF023288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramidase family protein"
                     /protein_id="HOE42070.1"
                     /translation="MNFIGKHQPLTAAGLGAALDHMDMQASEAAAIWAIVDVETSGIT
                     QGCGFRVDGRPQILYERHILRRETKGRFDQSAPHLSGPAGGYGGLATQYTKLEEALAL
                     CISAGLGREPALRAASWGLGQIMGFNAGLAGFGSAEAMVGAMLDGEDAQLLAMAKFLR
                     SQNLHKALLRRDWAAFARGYNGPSYAQNRYDIKLEQSYARLASGSLPNLQLRAAQVAL
                     LYLGFAPGKIDGVVGQRTRGALGNFRLQAGLSAGNELDGPCYLALCEKAGIRP"
     gene            1140..1442
                     /locus_tag="PLB25_10645"
     CDS             1140..1442
                     /locus_tag="PLB25_10645"
                     /inference="COORDINATES: protein motif:HMM:NF019228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42071.1"
                     /translation="MSHSPPGIGKRNIIEHRMVSYISQNWRGRPLVSIETIIHSIGST
                     TTKAGRQIQTAVDTATYAKGINFTDEEVATPDIRKNDFLGEQNDTIGPVKLDLVIS"
     gene            1734..2753
                     /gene="dctP"
                     /locus_tag="PLB25_10650"
     CDS             1734..2753
                     /gene="dctP"
                     /locus_tag="PLB25_10650"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOE42072.1"
                     /translation="MDSVLFAIGALLVAWMGAAWAADASTPLVFRISTENTASHVQTR
                     VVQRFADTLARRSQGRLVVEFKHSAQLFRDQDVIRALHQGKVDMAVVGTWQLDRYDPY
                     VSVLMLPMFFCLEAADHYRVRDGPVGQLISAHLQESLQVVVPGRWIDLGHAHLYATGA
                     AVAGYPDMAGLRIRIAGGEAIAAQMVAVGAVPVVVAWPDLPASLQRGRLDGLLTTHET
                     IASAQLWQHGIRNATEIKAYFAQYIPLISRNYWNQLQGDLRAMVQDSWESGVTQARLE
                     AAQAQSAARRTLEAHGVRVQQPSKEAMQAWRQRGLAAQGAIAQQLGVPETLINQAQQA
                     LLQTP"
     gene            2750..>4582
                     /locus_tag="PLB25_10655"
     CDS             2750..>4582
                     /locus_tag="PLB25_10655"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE42073.1"
                     /translation="MKTPFANPRAVQSLRLPQRRVLAFVVMLVLVTLAALAWKAIDDR
                     QKEQAIASGALQLQSQALSGVVDAAFGSAEQTLSNLAEQLQALPAGGTFGDSSGLRDK
                     LLKSPFLQDLRFYAADGHLLSRAGLAPLASHNLPDWLTHAQAQGRWIGLGGAPTELAI
                     FQTVRTADARVLGTFMASFASRYFDGVAEQLPGAGVEASLLLGSANELLLDMNRAANP
                     TGPAATPALLAPAAALAPGQGAGVFDLGGKLVVIQQLRNQPVRLVQVVSKDAALARWT
                     AQLIQSAMIAAVMVLAAALFLLFWKRSAQAQQGLLAELHLMSLAIEQTPAAVVFTDMK
                     GDIIYTNAAFTNATGYTQDEAKGQNPRILQSGTTPDDRYQEMWDKLTHGLPWTGTLLN
                     RRKDGSTFWEQSVIMPLRDTAGQATHYFAVKIDVSERIAAQDSARALLAEQQRLLKSI
                     PVGVYKYRVCASGQDRFDYVSDRLCQDLGLQAADLLLDPRLAFDLYQPQDLDRLRKAT
                     ATALATDGHMVFEGPLKGDAAKARWLRLESVLTRQDNGDQVWDGVQSDITERKQTEIA
                     LLEKARELLRSNSELEQFSYSISHDLRQPLRMVSSYLQLLHKGLG"
     assembly_gap    4583..4592
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4593..5199
                     /locus_tag="PLB25_10660"
     CDS             <4593..5199
                     /locus_tag="PLB25_10660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE42074.1"
                     /translation="GDALDAEKRQFFDFAIEGAKRMDQMMLGLLDYSRVGRKGEPPVW
                     LDNRAVLDEAMLFLRPLAAEAQADIRIDGDWPRILASPNELLRLLQNLLGNALKFRVA
                     NRLPQITVTSSTATAAGGEWQVCIADNGVGIAPEQIGRLFQVFARLHSRAAYEGTGIG
                     LALCRKIAEHHGGSIRAESEGEGQGSRFCLVLPLSQEEKPP"
     gene            5196..>6404
                     /locus_tag="PLB25_10665"
     CDS             5196..>6404
                     /locus_tag="PLB25_10665"
                     /inference="COORDINATES: protein motif:HMM:NF017081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HOE42075.1"
                     /translation="MKRLVQTPEQICPESSGHAEAAKPSAGRLLTVAAAYFITGWLGL
                     QISIIGSNITLIWLPTGIAVAALLRWGPRYWPAVWLAALAVNLAIGSSLTVAGAISIG
                     NTLAPVASAALLRRWQFNPQMLNMRDIPVFVAGGALLAMMISASWGVATLLVGGLLPP
                     QATVWAWTTWWLGDAVGVIVAGLALVTFERQELHRLLSVKLRRELGLSLACVALVGWG
                     WANSHANPLGDTAMFTIAIPTVVWLAMRLGPWAASTAVLMLSSCAALGLLAGGHQLQG
                     APLHQAILVLWSHMTMLAVLTMLVAALTAERRRMEGQLRQSELLYRTIADFTADWEYW
                     LLPDGTLHYMSPSCAQISGYSAAQFYADPQLLTSIVHPEDALLVDGHRHHLSSQGVPL
                     PIDFRILTQDG"
BASE COUNT         1162 a         1952 c         2041 g         1239 t
ORIGIN      
        1 gactgccgcg atctgactga caagcaccac aaggagactg gacatgaatt tcattggcaa
       61 acaccaaccc ctgaccgccg ccggccttgg cgcggcgctt gatcacatgg acatgcaggc
      121 cagcgaggcc gcagcgatct gggccatcgt cgatgtcgag acttcgggca tcacgcaggg
      181 ctgcggtttt cgcgttgacg ggcgaccaca gatcttgtac gagcggcata ttctgcggcg
      241 cgagacgaaa gggcgttttg accagagcgc gccacacctg agcggcccgg ccggcggcta
      301 cggcggtctg gcgacccagt acaccaagct ggaggaggca ctggcgctgt gcataagcgc
      361 tggcctcggg cgtgagccgg cgttgcgcgc agcatcttgg gggttgggcc aaatcatggg
      421 cttcaatgcc gggttggccg gtttcggcag cgccgaggcg atggttggcg ccatgctcga
      481 cggcgaggat gcccagcttc tggcaatggc gaaattcctg cgttcgcaga atctgcacaa
      541 ggcgctgctg cggcgcgact gggccgcttt cgcgcgcggc tacaacgggc cgagctatgc
      601 gcaaaaccgc tacgacatca agcttgagca gtcctatgcc cgcctggcat ccggatcact
      661 ccccaacctg cagttgcgcg cggcgcaggt ggcgctgctc tacctcggct ttgcgccggg
      721 caagatcgac ggcgtggtcg gccagcgtac tcgcggtgca ctcggcaact tccggctgca
      781 ggccggtttg tcggccggca acgaactcga cgggccgtgt tacctcgcgc tgtgcgaaaa
      841 ggcgggcata cgcccgtgac cccgtgcgac tgaggcgcgt gcccgaatac cggcgtgccg
      901 caccttgcgg catcttctgt atggtgaagc aggtcgtatt tgccaatatt ggcatcagta
      961 cggttacgcc agaatttgca gtgcagcgca tccgtcgccg gtgctcaagc gcaggctgct
     1021 gaccacaact gagcacaacc aatcaccgct gatggcggcg gtggcaatgg caccagagtg
     1081 cggctttgga aaacacaatt gcaaagcctt gccaatgaac ccggcttggt gatttcagcg
     1141 tgtcgcattc ccccccaggc attggcaaga ggaacataat cgaacatcgc atggtgtcgt
     1201 atatcagcca aaactggcgt ggtagaccgc tggtcagcat tgagacgatc atccattcaa
     1261 ttggttctac gaccaccaaa gccggccgac aaatccagac tgctgtagat acagccacct
     1321 atgcaaaagg aatcaacttc acggatgaag aagtggccac acccgacatc cgcaagaatg
     1381 actttcttgg ggaacagaac gacacaattg gcccggtaaa acttgatctt gttatttcgt
     1441 gatacatcgt tgagtgtctt tgttggaagg ataagttgcc ccacatcgtg ttaactcttg
     1501 cgcgctacac atcgactgct gcaacgtccg tgtcgcaatc ttcggcgcag gcggcaatgc
     1561 tgcagagcgc acaagtagat cgctggccaa agtcgggggt gctttggctt gtggcatcgg
     1621 tagcctgcct ggcaatgctg gcctgatcta tcgcattgcg ccggtcttta tgtatgaaat
     1681 atgcctctag cccccgtcct tgctgcgtga gcagctatca aaacaggatt tttttggatt
     1741 ctgttctgtt tgccattggt gcacttctgg tcgcctggat gggtgcggcc tgggccgctg
     1801 atgcgtccac gccgctggtt ttccggatct ccaccgaaaa caccgccagc catgtgcaaa
     1861 ccagggtggt gcagcgtttt gccgacacgc tggcgcggcg cagccagggg cgcctggtgg
     1921 tggagttcaa acacagcgca caactgtttc gggaccagga cgtgattcgt gccctgcacc
     1981 agggcaaggt ggacatggcg gtggtcggca cctggcagct tgaccgttat gacccctatg
     2041 tcagtgtgct gatgctaccg atgttttttt gcctggaggc agccgaccac taccgggtgc
     2101 gtgacggacc ggttgggcaa ctgatctcgg cgcatctgca agagtcgctg caagtggtgg
     2161 tgccggggcg ctggattgac ctcgggcacg cccaccttta tgccaccggg gcggcggtgg
     2221 cgggctaccc ggatatggct ggcctgcgca tccgcattgc tggtggtgag gcgattgcgg
     2281 cgcagatggt ggccgtcggg gccgtgccgg tggtggtggc ctggcccgat ttgcccgcca
     2341 gcttgcagcg tggccggctc gacggcttgc tgaccaccca cgaaaccatt gccagcgccc
     2401 agttgtggca gcacggcatt cgcaacgcca ctgagatcaa ggcttatttt gcccagtaca
     2461 tcccgctgat ttcacgcaac tactggaacc agttgcaagg cgacctgcgg gccatggtgc
     2521 aagacagttg ggaaagcggt gtcacccaag cccggcttga agccgcccag gcgcaaagcg
     2581 cggcacgccg cacgctcgaa gcccacggtg tgcgggtgca acagccgtcc aaagaggcga
     2641 tgcaggcgtg gcgccagcgt ggcctggccg cgcaaggcgc gattgcgcaa cagctcggcg
     2701 tgcctgaaac actgatcaac caggcgcaac aggcgctgct gcaaacccca tgaagacacc
     2761 ttttgccaac ccgcgcgcgg tgcagtcctt gcgcttgccg cagcgccggg tgctggcttt
     2821 tgtcgtcatg ctggtgctgg tgacgctggc ggcactggcc tggaaagcca ttgatgaccg
     2881 gcaaaaagag caggctattg ccagtggcgc gctgcaactg caaagccagg cgctcagcgg
     2941 cgtggtcgat gccgcgtttg gctcggccga acagaccctg tcgaatctgg ccgagcagct
     3001 tcaggcgctg cccgccggtg gcacctttgg cgattcatcg ggcttgcgcg acaagctgct
     3061 gaaatcgcct tttttgcagg atctgcgctt ttatgctgcc gatggccacc tgctctcgcg
     3121 cgccgggctg gccccgctgg ccagccacaa cctgcccgac tggctgaccc acgcccaggc
     3181 gcagggccgc tggattggtc tgggtggcgc gcccaccgag ctggcgattt ttcagaccgt
     3241 gcgcaccgcc gacgccaggg tgctgggcac cttcatggca agctttgcca gccgttattt
     3301 tgacggtgtt gccgagcaat tgccgggggc tggggtcgag gccagcttgt tgcttgggtc
     3361 ggcaaacgaa ttgctgctgg acatgaatcg cgctgccaac ccgactgggc cggcggccac
     3421 accagcactg cttgcgccag ccgctgcttt ggcacccggc cagggtgccg gggtttttga
     3481 cctgggcggc aagctggtgg tgatacagca gttgcgcaac cagccagtcc ggctggtgca
     3541 agtggtctca aaagatgccg ctttggcgcg ctggacggcg caactgatcc aaagtgccat
     3601 gatcgccgcc gtgatggtgc tggcggcggc cttgtttcta ctgttttgga agcgctcagc
     3661 acaagcccag caaggcctgc tggccgagct gcacctgatg agcctggcca tcgaacaaac
     3721 cccggcggca gtggtgttca ctgacatgaa gggcgacatc atctacacca acgcggcgtt
     3781 tacaaatgcc accggctata cgcaagacga ggccaaaggt caaaacccgc gcatcctgca
     3841 atctggcacg acacccgatg accggtacca ggagatgtgg gacaagctga cccacggctt
     3901 gccctggacc ggcacgctgt tgaaccggcg caaagacggc agcaccttct gggagcaaag
     3961 tgtgatcatg ccgctgcgtg acacggccgg gcaggccacg cactattttg ccgtcaagat
     4021 tgacgtgagt gagcgcattg cggcgcaaga ctcggcccgt gcgctgctag ccgagcagca
     4081 gcgcctgctc aagtcgattc cggtgggggt ctataaatac cgggtttgcg cctcggggca
     4141 agaccggttt gactatgtca gtgatcgcct gtgccaggac ctcgggctgc aggccgccga
     4201 tttgctgctt gacccgcgcc tggcttttga tttgtatcag ccacaagacc ttgaccggct
     4261 gcgcaaggcc acggccacgg cactggcgac cgacgggcac atggtttttg aagggccgct
     4321 caagggcgac gcggccaagg cgcgctggct gcgcctggag tcggtgctga cgcggcaaga
     4381 caacggcgac caggtctggg acggtgtgca gtccgacatc accgagcgca aacaaaccga
     4441 aatcgccctg cttgaaaaag cccgggagct gctgcgctcc aacagcgagc ttgagcagtt
     4501 cagctacagc atctcgcacg acctgcgcca gccgctgcgc atggtgtcga gctacctgca
     4561 actgctgcac aagggcctgg gtnnnnnnnn nngggcgacg cgctggatgc cgaaaaacgc
     4621 cagtttttcg actttgccat tgaaggtgcc aaacgcatgg accagatgat gctggggctg
     4681 ctcgactact caagggtggg ccgcaagggc gagccaccag tctggctcga caaccgtgcc
     4741 gtgctggacg aagccatgct gtttttgcgc ccgctggcag ccgaggcaca ggccgacata
     4801 cgcattgatg gcgactggcc gcgcattctt gccagcccga atgagctgct gcgcctgctg
     4861 caaaacctgc tgggcaacgc gctcaagttt cgtgtcgcca accggctgcc acaaatcacc
     4921 gtcaccagca gcacagccac cgcggcaggc ggcgaatggc aggtttgcat tgcggacaac
     4981 ggtgttggca tcgcccctga gcagattggc cggctgtttc aggtctttgc ccgcctgcat
     5041 agccgtgccg cctatgaggg caccggcatt ggcctggcct tatgccgcaa gatcgccgag
     5101 caccacggcg gtagtatccg ggccgagtct gagggcgaag gccaaggtag ccggttttgt
     5161 ctggtattgc cgctgtcaca agaggaaaaa cccccatgaa gcggctggtc cagacacccg
     5221 aacaaatttg tcctgaatcc agcggccacg ccgaagcggc caaaccatca gcagggcggc
     5281 tgctgacggt cgcggctgcc tatttcatca ccggctggct ggggctgcaa atttcgatca
     5341 ttggctccaa catcaccctg atctggctgc ccaccggcat tgcggtggcg gccctgttgc
     5401 gctggggccc gcgctattgg ccggcggtct ggctggcggc cctggcagtc aacctggcga
     5461 ttggctcgtc gctgacggtg gccggtgcca tatccattgg caacaccctg gctccggtcg
     5521 cgtccgctgc cctgttgcga cgctggcagt tcaacccgca gatgctcaac atgcgcgaca
     5581 taccagtatt tgttgctggc ggcgcattgc ttgccatgat gatcagcgcg agctgggggg
     5641 tggccacgct gttggtcggc ggcttgctgc cgccgcaggc gaccgtctgg gcctggacca
     5701 cctggtggct gggcgacgcg gtgggtgtca tcgtggctgg cctggccctg gtgacctttg
     5761 agcgccagga actgcaccgc ttgctcagcg tcaagctgcg ccgcgagttg ggcctgtcgc
     5821 tggcttgtgt ggcactggtg ggatggggct gggccaacag ccatgccaac cctttgggcg
     5881 acaccgccat gttcaccatc gccattccaa ccgtggtctg gctggcgatg cggctggggc
     5941 cctgggctgc ctccactgcg gtattgatgc tgtcgagctg tgccgcgctg gggctgctcg
     6001 ctggcggcca tcagttgcag ggcgcgccac tgcatcaggc gattcttgtg ttgtggtcgc
     6061 acatgaccat gctggcggtg ctgacgatgc tggtggctgc actgacggct gagcggcggc
     6121 gcatggaagg gcaattgcgt caaagcgagc tgctttaccg caccattgcc gatttcaccg
     6181 ctgactggga atactggctg ctgcccgacg gcaccctgca ctacatgtcg ccgtcgtgcg
     6241 cccagatcag cggctacagc gccgcgcagt tttatgccga cccgcaactg ctgacaagca
     6301 tcgttcaccc cgaagatgcc cttttggttg acgggcatcg ccatcacctc tccagccagg
     6361 gggtgccact gccgattgat ttccgcatcc tgacccagga cggt
//
LOCUS       DAOSLL010000221         6387 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11006_length_6387_cov_5.173405, whole genome shotgun sequence.
ACCESSION   DAOSLL010000221 DAOSLL010000000
VERSION     DAOSLL010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6387
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11006_length_6387_cov_5.173405"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..453
                     /locus_tag="PLB25_10670"
     CDS             <1..453
                     /locus_tag="PLB25_10670"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42076.1"
                     /translation="ASRASAWGHCTFSNLAIALLRLRAAGQIRSAFVVDFDLHTGDGT
                     KDVLRGWPEAQVVNTYGDNAAELIQNLAERLKQAPQVDILAVSAGFDAYIHDLGGKLA
                     TDDYFTIGRLLQGYAIRLGHQRRFAVLEGGYYLPDLGKNALAFCRGFE"
     gene            460..780
                     /locus_tag="PLB25_10675"
     CDS             460..780
                     /locus_tag="PLB25_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42077.1"
                     /translation="MKQIPKFTPNPKGSLAGYDRIDIDLPDARIGNIRCRLLAEKAIV
                     YSIQVFPEFQRNGYGAAAIDMLKARYPVLIADRVRFSAREFWEKMGFTVNAEGNWEYR
                     RGGR"
     gene            complement(797..1852)
                     /locus_tag="PLB25_10680"
     CDS             complement(797..1852)
                     /locus_tag="PLB25_10680"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HOE42078.1"
                     /translation="MSRIKVMVVDDSAVVRQVVTGLLAADPDIEVIAAVADPILAIAR
                     MKVQWPDVIVLDIEMPRMDGITFLKKVMAEKPTPVVICSTLVEAGAQTSMAALAAGAV
                     AIIAKPKVGLKQFLEDASEDVVSAVKAAAHANVKMLRATAKHTADVIMPAADKHSAMI
                     QTTERVVALGTSTGGTQALEVVLTSLPRVTPGIVIVQHMPEKFTAAFSERLNSLCQIE
                     VKEAKNNDRVVPGRALIAPGGKHMLLRRTGAQYYVEVMDGPLVNRHRPSVDVLFRSVA
                     KCAGANSLGVIMTGMGDDGAAGLMEMRTAGARTVAQDEESCVVFGMPKEAIKRGGVEK
                     TVTLTAISREIMQQMHL"
     gene            complement(1854..2396)
                     /locus_tag="PLB25_10685"
     CDS             complement(1854..2396)
                     /locus_tag="PLB25_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HOE42079.1"
                     /translation="MARISNLMDIFLQPGELFVGDANFQIRTILGSCVSITLWHAQLR
                     VGGMSHFLLPTRGAARGSAELDGRYGDEALELMFRDLRAAGANPLVCEAKIFGGGNMF
                     PGNQHARGQQAKGLSVGKRNGEAARKLLRQHGIEVVTESLYGIGHRQIIFDVSKGDVW
                     SRQVRPSSLEIDAESVEQGM"
     gene            complement(2398..3201)
                     /locus_tag="PLB25_10690"
     CDS             complement(2398..3201)
                     /locus_tag="PLB25_10690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HOE42080.1"
                     /translation="MKPITDAEFAQFQRFIFNAAGISMADAKKALVTGRLSKRLAVHQ
                     LDSFGDYFKLLSSDLHPGEVQMAVDLLTTNETYFFREIKHFEFLRAQAVKARSQAQPF
                     RVWSAASSSGEEAYSVAMVLADCMQTTPWDILGTDISTRVLEGARRALYSLERGRHIP
                     PEFLKRYCRKGTGQYDGMLLVIPELRQRVNFKHANLNATLPEFGKFDLVFLRNVMIYF
                     NNETKRQVVARVIATIKPGGYFCIGHSESLNDISTAVQMVAPSIYQKPL"
     gene            complement(3409..4914)
                     /locus_tag="PLB25_10695"
     CDS             complement(3409..4914)
                     /locus_tag="PLB25_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008444728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE42081.1"
                     /translation="MFTNMKLGVRLALGFGIVIVLLIISTTVGYLGLKMSAEDLQRLT
                     NDRFPKTVMANNIVDAINVNARSMRNALLVKKPEDAKKELDRIPEQRRIIGENFDKLE
                     AGIKSVKGLAVLKAAQDARAIYVKDLDRFLELQKAGKAAESVDLLVGDIRKSQGDYIK
                     AVAGIVDFQSKLMEDEGKEGTLEAEQAERNLLIIAGIAVLLAVAVAWWIIGSVLKQIG
                     GEPNYARDMVTRVAGGDLSVKLVTHPKDESSLLYAMKMMIARLSQVVTDVNAGAQALA
                     SASEQVSATAQSLSQAASEQAAGVEETSASIEQMTSSIAQNTENAKITDGMASKAAKD
                     AVDGGEAVNATVEAMKQIAKKIGIIDDIAAQTNLLALNAAIEAARAGEHGKGFAVVAA
                     EVRKLAERSQVAAQEIGEVAGNSVGLAEKAGKLLAEIVPNIRKTSDLVQEITAASTEQ
                     SSGVGQINSAVGQLNQTTQQNASSSEELAATSEEMSGQAEQLQQIMMFFKL"
     gene            complement(5156..5719)
                     /locus_tag="PLB25_10700"
     CDS             complement(5156..5719)
                     /locus_tag="PLB25_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOE42082.1"
                     /translation="MTALVKTDRNLTPALKPAQTVVEAKQYLTFMLGGEMFSINILSI
                     KEIIWYANLTEVPMMPACIRGVINLRGAVVPVMDLSSRFGKPSTPVTKSTCIVIIEIE
                     TQTEGERQNMGVVVDSVQAVLEIASSEIEQAPSFGTKIRPDFIEGIGKVNGKFVILLN
                     VNQVLSMEEIGQMGQVTASAQGMAPGV"
     gene            complement(5731..>6387)
                     /locus_tag="PLB25_10705"
     CDS             complement(5731..>6387)
                     /locus_tag="PLB25_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE42083.1"
                     /translation="DGGEAVNATVDAMKQIAKKIGIIDDIAAQTNLLALNAAIEAARA
                     GEHGKGFAVVAAEVRKLAERSQVAAQEIGEVAGNSVELAEKAGRLLAEIVPNIRKTSD
                     LVQEITAASTEQSSGVGQINSAVGQLNQTTQQNASSSEELAATSEEMSGQAEQLQQTM
                     SFFKLDSSALGGAGSFAVRKSTASGKKAAAAKSVKAKPVTKAAQAPASELDESQFTKF
                     "
BASE COUNT         1215 a         1956 c         1758 g         1458 t
ORIGIN      
        1 gccagccgcg cctcggcctg gggccattgc accttcagca acctggccat tgcgctgctg
       61 cggctgcgcg cggccgggca gattcgctcg gcctttgtgg tcgatttcga cctgcatact
      121 ggcgacggca ccaaggatgt gttgcgcggc tggcccgagg cgcaagtggt caacacctac
      181 ggcgacaatg ccgcagaact gattcagaac ctggcagaac gcctgaagca agcgccgcaa
      241 gtggacattc tggcggtttc agccggtttt gatgcctaca tccacgatct gggtggcaaa
      301 ctcgccacag acgactactt caccatcggt cgcctgctgc aaggctacgc catccgtctg
      361 gggcatcagc gccgttttgc ggtgctcgaa ggggggtatt acctgcccga cctgggcaaa
      421 aacgcactcg ctttttgccg tggttttgag taaatggcca tgaagcagat tcccaaattt
      481 acccccaatc cgaagggctc cctggctggc tacgaccgca tcgacattga cctgccggat
      541 gcccgcatcg gcaatatccg ctgtcgcttg ctggccgaaa aggccattgt ttactccatt
      601 caggtgttcc ccgagttcca gcgcaacggc tatggtgccg ccgccattga catgctgaaa
      661 gcccgctacc cggtgctgat cgccgaccgg gtgcgcttct cggcgcgtga attctgggaa
      721 aagatggggt ttacggtcaa cgctgaaggc aattgggaat accggcgggg tggccgatag
      781 ttgcccctgc ggcccgtcac aaatgcattt gctgcatgat ttcgcggctg atggcggtca
      841 gggtgacggt tttttcgaca ccaccgcgct tgatggcttc tttgggcatg ccaaacacca
      901 cgcagctttc ttcgtcttgc gccaccgttc tggcccctgc cgtgcgcatt tccatcagcc
      961 cggcggcgcc gtcatcgccc atgccggtca tgatgacacc gagcgaattg gcaccggcac
     1021 atttggccac cgaccgaaac agcacgtcca ccgatggccg atgccggttg accagcgggc
     1081 cgtccatcac ctccacgtaa tactgcgcgc cggtgcgccg cagcagcatg tgtttgccac
     1141 ccggcgcgat caaggcccgc ccaggcacca cccggtcgtt gtttttggcc tctttcacct
     1201 cgatctggca caggctgttg aggcgctcgg aaaaagcagc ggtaaatttc tcgggcatgt
     1261 gctgcactat gacaatgccg ggggtcacgc gtggcaggct ggtcagcacc acctcaagtg
     1321 cctgggtgcc gccggttgaa gtgccaagtg ccaccacccg ttcggtggtc tggatcatgg
     1381 ccgagtgctt gtcggcggct ggcatgatca catcggcagt gtgtttcgcg gtcgcgcgca
     1441 acatcttgac attggcgtgc gcggccgcct tgactgcgct gaccacgtct tctgatgcat
     1501 cctccagaaa ctgcttcaag ccgactttgg gtttggcaat gatggccacc gcaccggctg
     1561 ccagcgccgc catcgaggtc tgcgcgccag cttccaccag ggttgagcaa atcaccaccg
     1621 gggtcggctt ctcagccatg acctttttga gaaaggtgat gccgtccatg cgcggcatct
     1681 cgatgtcgag cacgatcacg tcgggccact gcaccttcat acgggcaatc gccagaatcg
     1741 ggtcggccac cgcagcgatc acctcgatat ccgggtcggc cgccagcaag ccggtgacca
     1801 cctgacgaac cacggcagaa tcatccacca ccatcacttt gatacggctc acgttacatc
     1861 ccttgttcta cagactctgc gtctatttcc agcgaggacg gcctgacctg gcgtgaccag
     1921 acatcgccct tgctgacatc aaaaatgatt tgccgatgcc caatgccata caggctttcg
     1981 gtcacgacct caatgccatg ctggcgaagc agcttgcgag ccgcttcacc gttgcgtttg
     2041 cccaccgaca ggcctttggc ttgctggccc ctggcgtgct ggttgccggg aaacatattg
     2101 ccaccaccaa aaatcttggc ttcgcacacc agcgggttgg cccctgcggc gcgcaggtcg
     2161 cgaaacatca gttcaagtgc ctcgtcacca tagcgcccgt caagctcggc gctgccacgt
     2221 gccgcacccc tggttggcag caaaaagtgc gacatgccgc ccacgcgcag ttgcgcatgc
     2281 cacagcgtga tcgacacgca agagcccaag atggtgcgaa tctgaaaatt ggcatcaccg
     2341 acaaacagct cccccggctg caaaaaaatg tccatcaaat tgctgatacg cgccatgtca
     2401 caagggtttt tggtaaatcg acggtgccac catctgcacc gccgtgctga tgtcgttgag
     2461 gctttccgag tggccaatgc aaaaatagcc acctggcttg atggttgcaa tgacccgcgc
     2521 caccacctgg cgtttggtct cgttgttgaa ataaatcatc acgttgcgca aaaagaccag
     2581 gtcaaacttg ccaaactctg gcagcgtggc gttcaggttg gcatgcttga agttgacccg
     2641 ctggcgcagc tcggggatga ccagcaacat gccgtcatat tgcccggtgc ctttgcggca
     2701 gtagcgcttt aaaaattctg gtggaatatg ccggccgcgc tccagcgagt acagggcgcg
     2761 gcgggcacct tcgagcaccc tggtgctgat gtcggtgccc aaaatgtccc agggcgtggt
     2821 ttgcatgcag tcggccagca ccatggccac gctataggcc tcttcgccgc tggaactggc
     2881 cgcgctccat acccgaaacg gctgcgcctg gctacgcgcc ttgacggctt gcgcacgcaa
     2941 aaactcaaag tgcttgattt ccctgaaaaa ataggtttcg ttagtggtca gcaagtccac
     3001 cgccatctgc acttcacccg gatgcaagtc agacgacagc aacttgaaat agtccccgaa
     3061 gctatcaagc tgatgcaccg cgagtcgctt gctcaggcga ccggtgacca gcgccttttt
     3121 ggcatcggcc atcgaaatgc cggcagcatt gaatatgaac cgctgaaact gggcaaattc
     3181 ggcatctgtg attggtttca taagcttaat tgccttctag ccctttctgt atatgcgctg
     3241 caagctacta ttttaatagt atcaagcggg cttgcagcag cttgactcga ctgccagaca
     3301 acctgacctg aagccactgg acagccgtgc tggtcggttt ggccgacgca atcggcccaa
     3361 acaggcattc gaaagcgcaa gttcggtcgt attcatcaaa atcccgggtc aaagcttgaa
     3421 gaacatcatg atttgctgca gttgctcggc ctgaccgctc atttcctcag aggtggcggc
     3481 caattcctcg gagcttgacg cgttttgctg ggtcgtctgg ttcaattggc cgactgccga
     3541 gttgatctgg ccgacaccgc tggactgctc ggtcgaggcg gcggtgatct cttgcaccag
     3601 gtcactggtc ttgcggatat tgggcacgat ctcggccagc aatttgccag ctttttcagc
     3661 taggccgacg ctgttgccgg ccacttcgcc aatctcttgc gcggccacct ggctgcgctc
     3721 ggcgagcttg cgcacttcgg cggccaccac cgcaaagcct ttgccatgct caccagctct
     3781 ggcggcttca attgcggcgt tcagggccag caggttggtc tgggctgcaa tgtcgtcaat
     3841 gatgccgatc ttcttggcaa tctgcttcat ggcttcgact gtggcgttga ccgcttcacc
     3901 accatcgacc gcgtctttgg ccgccttgct ggccatacca tcggtgatct tggcgttttc
     3961 ggtgttttgc gcaatgctgc tggtcatttg ttcaatgctg gctgaggttt cttccacacc
     4021 tgctgcctgt tcgctggccg cttggctcaa actctgggcc gtggcgctga cctgttcaga
     4081 cgcgctggcc agcgcttgtg cgccggcatt gacatcggtc accacctgcg agagtctggc
     4141 gatcatcatc ttcatggcat acagcaaact gctctcatcc tttggatgtg tgaccagttt
     4201 gacactcaag tcgcccccgg ccacccgggt caccatgtca cgggcgtagt taggttcgcc
     4261 gccaatttgt ttcagcacac tgccaatgat ccaccaggcc accgccaccg ccaacagaac
     4321 ggcaatgccc gcaatgatca gcaaattgcg ctcggcttgc tcggcttcaa gcgtgccttc
     4381 cttgccctcg tcttccatca gcttgctctg aaaatccacg ataccggcca ccgccttgat
     4441 gtagtcgccc tgggatttgc ggatgtcgcc taccagcaag tccaccgact cggcggcttt
     4501 gccagccttt tgcagttcca gaaaacggtc aaggtccttg acgtaaattg cgcgagcgtc
     4561 ttgcgccgct ttcaacaccg ccaggccctt gacagatttg ataccggcct caagcttgtc
     4621 aaaattctcg ccaatgattc ggcgctgctc aggaatgcgg tcgagttcct ttttggcatc
     4681 ttcgggcttc ttgaccagca gcgcattgcg catggagcgg gcgttgacgt tgatcgcatc
     4741 cacaatgttg ttggccatga cggtcttggg aaagcggtcg ttggtcagtc gttgcaagtc
     4801 ttcggcgctc atcttgagcc cgaggtagcc caccgtggtg ctgatgatga gcagcacaat
     4861 gacaatgcca aagcccaatg ccagtctgac acccagtttc atattggtaa acatttattt
     4921 ttctccgtct gaaaacatgt cgccgccatc cctaagccgg ccgagccggg atcgatgagc
     4981 gttcgtcgcg agggcgcgcc tcctacacaa acagtcggcg ccaatttgaa tttgtaggag
     5041 tcgcgccctc gcgacgaatg actttgaatg tttgaattct tcgcatgatg tgtaaagact
     5101 tgacttgtta gagactaaga caaaggggtc cgactgcgtc cgaacccgtc ctttgtcaga
     5161 cacccggtgc catgccctgg gctgatgccg tcacttgccc catttgcccg atttcttcca
     5221 tcgacaacac ctggttgaca ttgagcaaga tgacaaactt gccgttgacc ttgccaatgc
     5281 cttcaatgaa atcggggcgg atcttggtgc caaagctggg ggcctgttcg atttcgcttg
     5341 acgcaatctc cagcaccgcc tgcaccgaat ccaccaccac ccccatgttc tggcgttcgc
     5401 cctcggtctg ggtttcgatc tcgatgatga caatgcaagt gctcttggtc accggggtgg
     5461 acggcttgcc aaagcggctc gacaagtcca tcaccggcac caccgcgcca cgcaggttga
     5521 tcacgccgcg aatgcacgcg ggcatcatcg gcacctcggt caggttggcg taccagatga
     5581 tttccttgat gctcaggatg ttgatcgaga acatctcgcc accgagcatg aaggtcaggt
     5641 actgtttagc ttccaccacc gtttgcgccg gtttgagcgc gggggtcaga ttcctgtcgg
     5701 ttttcaccaa agcagtcatg gcagattcct ttagaacttg gtgaactggg attcatccaa
     5761 ttcagatgct ggtgcctggg ccgccttggt cacaggtttg gctttgacgg actttgcggc
     5821 ggcggctttt ttgccgctgg cggtgctctt gcgcaccgca aaagaccctg cgccgccaag
     5881 cgctgaacta tcgagcttga agaacgacat ggtttgctgc aactgttcgg cctgaccact
     5941 catttcttcc gaggtggcag ccagctcttc ggagcttgac gcgttttgct gggtggtctg
     6001 gttcaactgg cccaccgccg agttgatttg cccgacaccg ctggactgct cggtcgatgc
     6061 tgccgtgatc tcctgcacca ggtcggaggt cttgcggatg ttgggcacga tctcggccaa
     6121 caagcggcca gctttctcag ccagttcgac gctgttgcca gccacttcgc caatctcttg
     6181 cgctgccacc tggctgcgtt ctgccaattt gcgcacttcg gcggccacca cggcaaaacc
     6241 cttgccatgt tcaccggcac gggcggcttc aatcgctgcg ttcagggcca gcaagttggt
     6301 ttgcgctgca atgtcgtcaa tgatgccaat cttcttggcg atctgtttca tggcatctac
     6361 ggtggcgttg acagcctcgc caccatc
//
LOCUS       DAOSLL010000222         6377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11025_length_6377_cov_4.674945, whole genome shotgun sequence.
ACCESSION   DAOSLL010000222 DAOSLL010000000
VERSION     DAOSLL010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11025_length_6377_cov_4.674945"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="PLB25_10710"
     CDS             complement(<1..765)
                     /locus_tag="PLB25_10710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE42084.1"
                     /translation="MSKTLLAGYRLARLGAHLVRGVVIVAWHFPNLSAVQKSERIQAW
                     ALALLAQVAIKFEVNGTPPASGPMLLVANHISWLDIAMLLAAGHCRFVAKSEIAHWPL
                     LGLLTRAAGTLFIARESRRDAFRVVHHMAEELRSGQVLAIFPEGTTSDGRHMLPFHAN
                     LFQSAISAHAPVQPVALRFVDAATGQLSLAPCYIDDDSLLGSVWRTLKAPPLCATLTF
                     GAPQGSEGRERRALAGEVRTAIEGLLRTGSGNQIAPP"
     gene            complement(895..2868)
                     /locus_tag="PLB25_10715"
     CDS             complement(895..2868)
                     /locus_tag="PLB25_10715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HOE42085.1"
                     /translation="MFDFVRKHTKIMMTLMFLLIIPSFVLFGIDGYNKMGNSDVAVAR
                     VGGIDITQAQWDQAHKVEADRMRESRPNLDPKLLDSPEARYATLERLVRDQVLELAAT
                     KSALVTSDTRLARYLQEAPAIASLRKADGKLDMERYRQLAASQGLTPEGFENSVRRDL
                     AKQQIVQGVNLTGFATPAVADVALNAFFERREVQVLNFLPASYAAQVNPTEAELEAFY
                     QANPALFQAPEQVQIEYVVLDLEAVKKGIRISEPDLKSYYDQNVARLSGNEERRASHI
                     LISVPKDAPAAEREKAKARADDLLKKARLAPDSFAELARQNSQDAGSAAKGGDLEFFQ
                     RGAMVKPFEDAAFAMKAGDISDVVASDFGYHIIRLTDIKTPKQRSFEELRPSIEGDLK
                     TQQAQRKYAEMAELFTNTVYEQSDSLKPVADKLQLDIMTVSNLLRHPGPDAKGVLASE
                     KLLSAVFSPESIGKKHNTEAVETAVNQLVAARVLSHSPARTQAQDEVRQLVRERVVAA
                     RANALAKKEGSEKLALWKADPTKASLPAALVVSRDQSQGVAPQIVSAALRLDATLLPA
                     FLGVDMGATGYALVRVNKRVARPAAVQANAQQEREQYAQWWTAAEAQAYYTTLKERLN
                     VQMLVSAPAKSVPVSAKPGTPAPPEPAATSASK"
     gene            complement(2929..3004)
                     /locus_tag="PLB25_10720"
     tRNA            complement(2929..3004)
                     /locus_tag="PLB25_10720"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2969..2971),aa:Val,seq:tac)
     gene            complement(3021..3293)
                     /locus_tag="PLB25_10725"
     CDS             complement(3021..3293)
                     /locus_tag="PLB25_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014191280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HOE42086.1"
                     /translation="MNKTELIDHIADTADISKAAAARALEATISAVQTTLKKGGSVSL
                     VGFGTFAVGKRAARVGRNPRTGASIKIKAAKVPKFRPGKALKDALN"
     gene            complement(3514..4092)
                     /gene="pgsA"
                     /locus_tag="PLB25_10730"
     CDS             complement(3514..4092)
                     /gene="pgsA"
                     /locus_tag="PLB25_10730"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HOE42087.1"
                     /translation="MFWTIPTIMTWTRIVAIPLIVGVFYLPIYLPISPTACNQIATVM
                     FVLFAATDWLDGYLARKLNQMSAFGAFLDPVADKFLVCACVLVLVHLQRADVFVGLII
                     IGREIAISALREWMAQIGASGSVAVHMIGKLKTTAQMVAIPFLLFDGMLLGVIDTHQW
                     GVWLIWISAVMTVWSMVYYLKKALPEIRSRVK"
     gene            complement(4134..6161)
                     /gene="uvrC"
                     /locus_tag="PLB25_10735"
     CDS             complement(4134..6161)
                     /gene="uvrC"
                     /locus_tag="PLB25_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HOE42088.1"
                     /translation="MPASVADAPAGHPAQLLSDVASLPGLPGVYRYFDAQGQVLYVGK
                     ANSLKKRVSSYFQKNHGGTRIGHMVSKIVRLETTVVRSEAEALLLENNLIKTLNPKYN
                     ILFRDDKSYPYLKISAPGPAVVADKPAGDQFPRVSYYRGAVEKKHHYFGPYPSAWAVK
                     EAILLMQKVFRLRTCEDPVFNNRTRPCLLYQIKRCSGPCVGHITPAAYAQQLQDAERF
                     LHGDAQQVMQALEARMMAHAERLEFEQAAELRNQVAALSNVLHQQSVDNVADRDVDIL
                     AVKVSGGRACVNLAMVRGGRHLGDRPYFPVQVQDAAGLDIDEDSASDELALALPLEAL
                     AGLPLVNKGMARSVESQVLDAFVAQHYLQVPVPSLLVVSAPVDKNLLQLLSVQTGSKL
                     SAVHQPREQRRVWLEMAQTNASLQLARLLAEEGSQQARTRALSEALDLLPDSLDELRI
                     ECFDISHTAGESTQASCVVFQHHKMQNAQYRRYKINDITPGDDYAAMRQVLLRRYGKL
                     SEALAQARQSGETPAGTGQMPDLVLVDGGRGQVSMAREVFEQLGLDLALIVGVEKGEG
                     RRIGLEELVFADGRDKVYLGKDSAALMLVAQIRDEAHRFAITGMRAARARVRMDGGRL
                     EEIAGIGPKRRARLLQRFGGVRGVAGASADDIASVEGISRELAEEIYRALH"
     gene            6289..>6377
                     /gene="earP"
                     /locus_tag="PLB25_10740"
     CDS             6289..>6377
                     /gene="earP"
                     /locus_tag="PLB25_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HOE42089.1"
                     /translation="MHLNPCLHWDIFCHVIDNHGDIGVCWRLAV"
BASE COUNT         1296 a         1958 c         1838 g         1285 t
ORIGIN      
        1 gggtggcgcg atttgattgc ctgaaccggt cctgagcaga ccctcgatag ccgtgcggac
       61 ttccccggcc agggcgcgcc gttctcgccc ctctgaccct tgcggcgcac caaaagtgag
      121 ggtggcacac agcggaggtg ctttcaaggt gcgccagacc gagcccagca gactgtcgtc
      181 atcgatgtaa cacggtgcca ggctcaattg gccggtggcc gcatcgacaa accgcaacgc
      241 cactggctgc actggtgcgt gagcagaaat cgccgactga aacaggttgg cgtgaaacgg
      301 caacatgtgc cggccgtcgc tggtggtgcc ttccgggaaa atagccagta cctgccccga
      361 gcgaagctct tcggccatgt gatgcaccac gcgaaacgcg tcacgccgtg actcgcgggc
      421 tatgaacagt gtgccggcgg cgcgggtcag caagcccagc agcggccaat gcgcaatttc
      481 agacttggcg acaaaacggc agtgaccggc cgccagcagc atcgcgatat ccagccagga
      541 aatgtggttg gccaccaaca acatcgggcc gcttgccggg ggggtgccat ttacttcaaa
      601 tttaatagct acctgcgcaa gcagcgcaag ggcccaagcc tgaattcgtt cagatttttg
      661 cactgctgac aagtttggaa agtgccatgc gacgatgacc acacccctga ccaaatgcgc
      721 cccgagccgt gccaggcggt agccagccag caatgtcttg ctcatgggca aaaaccggtg
      781 tcccgtccgg ctggcactgc actcgcctgc catctttgga ataacaagcc gccgctgggc
      841 attgtgtttt tgactccggt gtcgtccagg gagaagccgg gtgtggcctt tggcttactt
      901 tgaagccgac gttgccgctg gttcgggggg cgccggtgtg ccgggcttgg ctgacaccgg
      961 cacgctcttg gccggtgctg aaaccagcat ttgcacattc agacgttcct tcaatgtggt
     1021 gtagtaggcc tgcgcttccg ctgcggtcca ccactgggca tactgctcgc gctcttgctg
     1081 tgcgttggcc tgcactgcag ccggtcttgc cacccgcttg ttgacacgca ccagtgcgta
     1141 acccgtggcc cccatatcaa cacccaaaaa cgctggcagc aatgttgcat caagccgaag
     1201 cgcagcactg acgatctgcg gagcaacacc ctgcgactgg tcgcgcgaga ccacaagcgc
     1261 ggccggcaag gatgccttgg tcgggtcggc cttccacagc gccagctttt cgcttccttc
     1321 ttttttggca agcgcattgg cgcgcgccgc aacgacccgc tcacgcacca gttgacgcac
     1381 ctcgtcttgc gcttgtgtac gggccgggct gtggctcagc acgcgcgccg ccaccagttg
     1441 gttgacggcg gtttcaaccg cctcggtatt gtgctttttc ccgatcgact ccggggaaaa
     1501 aacggctgaa agcaactttt cgctggccag cacgcctttg gcatcgggtc ccgggtggcg
     1561 cagcaaattg ctgacggtca tgatgtcaag ctgaagtttg tcggcaaccg gtttgaggct
     1621 gtccgactgt tcgtagaccg tattggtaaa aagttcggcc atctcggcat atttgcgctg
     1681 tgcctgctgg gtcttgaggt caccttcaat gctcgggcgc aactcttcaa agctgcgttg
     1741 cttgggtgtc ttgatgtcgg tgagcctgat gatgtggtag ccaaaatcag aggccacgac
     1801 atcgctgata tcgcctgcct tcatggcaaa agccgcatct tcaaacggtt tgaccatggc
     1861 accacgctgg aaaaattcga ggtcgccgcc tttggccgca gaacctgcat cctgcgagtt
     1921 ctgcctggcc aattcggcaa aactgtccgg ggccagtcgc gcctttttca gcaaatcgtc
     1981 agcgcgggct ttggcctttt cccgctcggc tgccggggca tccttgggca cgctgatcag
     2041 aatatggctg gcccggcgct cttcgttgcc gctcagacgc gccacattct ggtcatagta
     2101 agacttgagg tctggctcgg aaatccggat acccttcttg accgcttcaa gatcaagcac
     2161 cacatattcg atctgcacct gctcaggtgc ctggaacagc gccggattgg cctgataaaa
     2221 agcctccagc tcggcctcag tcggattgac ctgggccgca tagctggccg gcagaaaatt
     2281 cagcacctga acttcacgcc gttcaaaaaa ggcattcaag gcaacgtcgg caactgccgg
     2341 cgtggcaaag ccggtcaggt tgaccccctg cacaatttgc tgcttggcaa ggtctcggcg
     2401 cacactgttt tcaaagcctt caggtgtcag accctggctg gcggcgagtt gccggtagcg
     2461 ctccatgtcc agtttgccat cagccttgcg cagcgatgct atcgccggtg cctcttgcag
     2521 atagcgtgcc agtcgcgtgt cactggtcac cagggcggac tttgtggcag ccagctccag
     2581 cacctggtca cgcaccaggc gttccagcgt ggcataacgg gcctctggtg agtcgagcag
     2641 cttcggatcc agattgggcc tggattcgcg catccgatcg gcttccacct tgtgcgcctg
     2701 gtcccattgg gcctgggtga tgtcgatgcc gccgacccga gctacagcca cgtcggagtt
     2761 gcccatcttg ttgtagccat caatgccaaa caaaacgaat gatggaatga tcaacaaaaa
     2821 catcaacgtc atcatgatct tggtgtgttt ccgaacgaaa tcaaacatgc gcaaaatctc
     2881 ctctagggca acaaaaaagg cgaactgttc gttcgccttt gtggattgtg gtgggtgatg
     2941 acgggctcga accgccgacc tacgccttgt aagggcgccg ctctaccaac tgagctaatc
     3001 accccaccga taaacagaaa tcaattcaag gcatctttca aagccttgcc gggacggaat
     3061 ttgggaacct tggctgcctt gattttaatc gatgcaccgg tgcgcggatt gcgaccgaca
     3121 cgggctgcac gcttgccaac cgcaaaagtg ccaaacccca ccaatgaaac cgagccgcct
     3181 tttttcagtg tcgtctgaac agccgagatg gttgcctcaa gtgcgcgtgc tgctgctgct
     3241 ttggagatat cggccgtatc ggcaatgtga tcaataagtt cggttttatt cacggcaagc
     3301 ctcaaacagg aatggaacac tactgagtac ccacagactt cccaacctgt atgtacgcca
     3361 cggtacatcc caaaacaacg accaatctgc agggaggttc acgactgcga gccgagattc
     3421 taagccgatt cacgcggccg atcagccaga ttaggcaaac tttattgctg gaagtcaaag
     3481 gcggctggaa ttctgccgct ggcgagcgac cgattacttc accctggagc ggatttccgg
     3541 cagggctttt ttcaggtaat aaaccatcga ccagactgtc atcaccgccg agatccagat
     3601 cagccagaca ccccactgat gggtgtcgat cacgcccagc agcatgccgt caaacagcag
     3661 aaaaggtatc gccaccattt gcgccgtggt tttgagtttg ccaatcatgt gcaccgccac
     3721 actgccggag gcaccaattt gggccatcca ttcgcgcagc gccgaaatgg caatttcgcg
     3781 accaatgatg atcagcccga cgaacacatc ggcccgttgc aagtgcacca gcaccagcac
     3841 gcaggcgcag accagaaact tgtctgccac cgggtccaga aacgcgccaa aggcagacat
     3901 ctggttgagc ttgcgcgcca gatagccgtc gagccagtcg gtcgcagcaa acagcacaaa
     3961 catgacggtg gcaatctggt tgcaggcggt gggcgaaatg ggcagataaa ttggcaggta
     4021 aaacacgccc acgatcagtg gaatcgccac aatgcgagtc caggtcatga tcgtcgggat
     4081 ggtccaaaac atggcgcaat tgtggcacgc acccggtacc ggtgcgggta ctgtcagtgc
     4141 aacgcccggt aaatctcctc ggccagttcc cttgagatgc cttcgaccga cgcgatgtca
     4201 tcggctgagg caccagccac cccacgcacc ccgccaaagc gctgcaacag gcgggcacgc
     4261 cgcttcgggc caatgcctgc aatctcttcg aggcgaccgc cgtccatgcg caccctggca
     4321 cgcgccgcac gcatgccggt gatggcaaag cggtgcgctt cgtcgcgaat ttgcgccacc
     4381 agcatcagcg ctgccgaatc cttgcccaaa tagaccttgt cgcggccatc ggcaaacacc
     4441 aattcttcca gcccaatgcg tcggccttcg cccttctcca caccgacgat cagcgccagg
     4501 tccagcccga gctgttcaaa cacctcgcgc gccatcgaca cctgcccgcg tccgccatcg
     4561 accagcacca gatcgggcat ttgcccggtg cctgccggtg tttcacccga ctgcctggcc
     4621 tgcgccaggg cttcggacaa cttgccatag cgccgcagca gcacctggcg catggcggca
     4681 tagtcgtcac ccggcgtgat gtcgttgatc ttgtagcgcc ggtactgggc gttttgcatt
     4741 ttgtggtgct gaaacaccac gcacgaggcc tgggtggatt cgccagcggt gtgcgaaatg
     4801 tcaaagcact cgatacgcag ctcgtcaagc gagtcgggca acaaatccag cgcttcggac
     4861 agggcgcggg ttctggcctg ttgcgagcct tcttctgcca gcagccgtgc caactgcaaa
     4921 ctggcgttgg tttgcgccat ttcgagccag acccggcgtt gctcacgcgg ctggtgcacc
     4981 gcgctgagct tgctgccggt ctgcacgctc aacagttgca gcaagttctt gtcgaccggg
     5041 gcacttacca ccagcaagga tggcaccggt acctgcaagt agtgttgtgc cacaaaagcg
     5101 tcgagcacct gcgactcaac cgaacgcgcc atgcccttgt tgactagcgg caagcctgcc
     5161 agcgcctcaa gcggcaacgc aagggccaac tcgtcgctgg cactgtcttc atcgatatcg
     5221 agccccgccg catcttgcac ctgcaccgga aaataaggcc ggtcgcccag gtggcggcca
     5281 ccgcgcacca tcgccaggtt gacacaggcg cgcccgcccg acaccttgac cgccagaata
     5341 tcgacatcgc ggtcggccac gttgtccacc gactgctggt gcaacacatt ggacagggcc
     5401 gcaacctggt tgcgaagttc tgcggcctgc tcaaattcga gccgctcggc atgcgccatc
     5461 atgcgggctt ccagtgcctg catcacctgc tgggcatcgc catgcaaaaa acgctcggca
     5521 tcttgcagtt gctgtgcata ggcggccggt gtgatgtgcc cgacgcaggg gccagagcag
     5581 cgcttgatct ggtacagcag gcacggccgg gtgcggttgt tgaacaccgg gtcttcgcag
     5641 gtgcgcagcc gaaacacctt ttgcatcagc aaaatcgctt ccttgacggc ccaggcgctg
     5701 ggataggggc cgaaatagtg gtgttttttt tcaaccgcac cccggtaata agacaccctg
     5761 ggaaactggt cgccggccgg tttgtcggca acgacggccg gaccgggcgc gctgattttg
     5821 aggtaagggt agcttttgtc gtcccgaaac aagatgttgt acttggggtt gagcgtcttg
     5881 atcaggttgt tttccagcag caaggcttcg gcctcggagc gcaccacggt ggtttccagc
     5941 cgcacgattt tgctgaccat gtgaccaatg cgggtgccgc cgtggttctt ctgaaagtag
     6001 ctcgacaccc gcttcttcag gctattggcc ttgccgacat acaaaacctg gccctgcgcg
     6061 tcaaagtagc gatagacacc cggcaagccg ggcagactcg ccacgtcgct aagcaactgc
     6121 gccggatgtc cggcgggtgc gtcggccacc gacgcaggca tggcagcggg cagtgcgctc
     6181 gccggtgcag gcggcgccgg tggcgtgacc gacgcaatca aaggggtttc aggcagatca
     6241 ggcatagggc gctattgtgg ggtgggcaca ttgtggacaa tcccggccat gcacttgaac
     6301 ccctgcctgc attgggacat tttttgccac gtgatcgaca accacggtga cattggcgtt
     6361 tgctggcggc tggcagt
//
LOCUS       DAOSLL010000223         6377 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11026_length_6377_cov_3.948118, whole genome shotgun sequence.
ACCESSION   DAOSLL010000223 DAOSLL010000000
VERSION     DAOSLL010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6377
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11026_length_6377_cov_3.948118"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="PLB25_10745"
     CDS             <1..482
                     /locus_tag="PLB25_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOE42090.1"
                     /translation="WQKELGLTFVHVTHSQEEAMALADTMVVMNHGVIEQMGGPHDIY
                     NRPLSEFVARFMGGHNVLDTPAGKISVRTDHMQLAADGTALAADSNHRRAIITDIEYQ
                     GSYVLVGLQNPGENLTASSTAELSVMVPEAVFTSRPYALGDNVHLTWQPQAAHPLRQ"
     gene            671..1858
                     /locus_tag="PLB25_10750"
     CDS             671..1858
                     /locus_tag="PLB25_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE42091.1"
                     /translation="MFPAIHAQEKIVLRYLGTAVNQDKAIGEKFKADTGIEIQYVAVT
                     TDDVTKRAVTAPNSFDLIDTEYFSLKKIVPTGNLKGIDVKKIKNADKITSLFTTGQVA
                     GKAVGDQGTAPKKVIYLEGEKSKVFAKSPTQFMSLIPTVYNADTLGIRPDLIKRPINS
                     WAELLNPEFKGKAAILNIPSIGIMDAAMVVEAMGLYKYPDKGNMTKKEIDLTIKTLIE
                     AKKAGQFRSLWKDFNESVNLMASGEVVIQSMWSPAVTAVRTKGIACNFQPLKEGYRAW
                     AAGFGLPTTLSGRKLDGAYEFINWFLDGWAGAYLNRQGYYSAVLETAKSKMEAYEWAY
                     WMEGKAATQDIKSPNGDVLAKTGATRDGGSYEARMGGIACWNAVMDENNYMVQKWNEF
                     VAA"
     gene            2092..3003
                     /locus_tag="PLB25_10755"
     CDS             2092..3003
                     /locus_tag="PLB25_10755"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE42092.1"
                     /translation="MNTRAASPPAAAPVTGRGIAAWWQATPLTLVFVLFFLIPLGLIG
                     MVSFWDFNEYELLPDFTVKHYLAIFEGCSNTDEMCVTFKTYLSTFKFSFLVWLITLLI
                     GFIVSYFLAFHVRSSNVQTLLFVLCTIPFWTSNVIRMISWVPLLGRNGLVNQSLVGAG
                     LIDTPIEWLLFSDFSVVLAFVHLYTMFMIVPIFNSMMRIDRSLIEAANDSGASAWQTL
                     WHVIVPLCRTGIIIGSIFVITIVMGDFVTIGVMGGQQIASVGKIIQVQTSYLQFPLAA
                     ANAVILLLLVLMIIWALTRLVDIRKEL"
     gene            3004..3888
                     /locus_tag="PLB25_10760"
     CDS             3004..3888
                     /locus_tag="PLB25_10760"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE42093.1"
                     /translation="MSNDKRSAGFWFLALFFAAFVLFLYGPMLVIVVLSFQGPEGGLT
                     FPLRGLSLHWFGKLAEGLGVVDIGAALKRSLGLGLAVMLLTVLFSVLAGLAFRKKLAG
                     GNALFYVVVASLIMPSIIVSLGIGLEFRLLDTGLKWALEFLGMTSTSESYGTSLGLYT
                     SALGAHLTWTLPFGLLIMFAVFNRFNPAFEEAARDLGATPWQALRHVVLPLIAPSVVG
                     IGMFGFTLSWDEIARTSQAIGDVNTLPLELQGLTTTVTTPSIYALGTVTTVISLGVMV
                     LAIGLARWLSKRQQQGKQ"
     gene            3885..4598
                     /locus_tag="PLB25_10765"
     CDS             3885..4598
                     /locus_tag="PLB25_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOE42094.1"
                     /translation="MSETAAAPPLTDGDMYERVISAILDHRLPPGTKLVEDKLASAFG
                     VSRTRIRPVLVRLANEQVVTLTPNRGATIAQPTEQEAREVFQVRSLIEPSLVELFIAR
                     ATETDMNNLKTCIDDEEAARKAGHMRRAIRLSGQFHTLIAEGANHLTLARILREMTSR
                     TTLILMTYSAAHEFERTDATSCGCDEHRALLDAIRLRDPLEAARRMRAHLARLQAQLR
                     FDVTQVDLPDLHALFGTAS"
     gene            4765..5502
                     /locus_tag="PLB25_10770"
     CDS             4765..5502
                     /locus_tag="PLB25_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HOE42095.1"
                     /translation="MRQLLVINPNTSPGVTALLRQHVQQAAGSHVVVTAVTARFGAPY
                     IADEASYAVAGHATLDAWAAALAQAAQPPDAVLIGCFGDPGLMALRESSPTPVTGLAE
                     AAFIEAARHGRFAIVTGGERWAPILQRLAQSLGHASGLAGIHTVAPTGAQLAADPIAA
                     RRLLTQACLDAVRQLGVQTVILGGAGLAGMAAGIQPFVNVPIVDSVTAGTHWALRSHV
                     TPPQRATPGFDVPWVNLSAELTGMSLR"
     gene            6173..>6377
                     /locus_tag="PLB25_10775"
     CDS             6173..>6377
                     /locus_tag="PLB25_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42096.1"
                     /translation="MLSNVMNALQLFLIPLLICLVVAAAVVMLVRRRRSPAAAPDRSN
                     LADPPTDDDSDFLDVAPPPPPGAV"
BASE COUNT         1270 a         1865 c         1919 g         1323 t
ORIGIN      
        1 gctggcaaaa agagctgggc ctgacttttg tccacgtcac ccactcgcag gaagaagcca
       61 tggcgctggc cgacaccatg gtggtgatga accatggcgt gatcgagcag atgggtggcc
      121 cgcacgacat ctacaaccgc ccgttgagcg agtttgtggc gcgctttatg ggcggccaca
      181 acgtgctcga cacgccagcc ggaaaaatca gcgtgcgcac cgaccacatg cagcttgccg
      241 ccgatgggac agcgctggcg gccgacagca accaccggcg cgccatcatc accgacatcg
      301 aataccaagg cagctacgtg ctggttggcc tgcaaaaccc aggtgaaaac ctgaccgcca
      361 gcagcaccgc cgagctgtcg gtgatggtcc ccgaagctgt gtttaccagc cgcccctacg
      421 ccctgggcga caacgtgcat ctgacctggc agccacaggc tgcgcacccg ttgcgccaat
      481 aactgtgccc caacctgtta cccccactgc ttcgaaagaa gatttttgtc agcccaccag
      541 gagtattgcc atgtcagacc tttccaaaac catcaccgac ttagccacca gcgtggccag
      601 cggcgcactc agtgccggtg tgccgcgacg cagcctgctc aagggcacgg ccggcattct
      661 ggccgctggc atgttcccgg caattcatgc gcaggaaaaa attgtgctgc gctacctggg
      721 cactgcagtc aaccaggaca aggcgatagg cgagaagttc aaggccgaca ccggcatcga
      781 aatccagtac gtggcagtaa ccaccgacga cgtgaccaag cgcgctgtga ccgccccgaa
      841 cagctttgac ttgatcgaca ccgagtactt ttcgctcaag aaaattgtgc ccaccggcaa
      901 cctcaagggc atcgatgtca agaaaatcaa gaatgccgac aagatcacca gcctgttcac
      961 caccggtcag gtggctggca aggccgtcgg cgaccagggc acggcaccca agaaggtgat
     1021 ttaccttgaa ggcgaaaaga gcaaggtgtt tgccaagagc ccgacccagt tcatgagcct
     1081 gattccgacc gtctacaacg ccgacacgct gggcatccgc cctgacctga tcaaacgccc
     1141 gatcaattcc tgggctgagt tgctcaatcc tgagttcaag ggcaaggccg ccattttgaa
     1201 cattcccagc atcggcatca tggatgccgc catggtggtc gaagcgatgg gcctgtacaa
     1261 gtaccccgac aagggcaaca tgaccaagaa ggaaattgac ctgaccatca agaccttgat
     1321 cgaagccaaa aaagccggcc agttccgcag cttgtggaaa gacttcaacg agtctgtcaa
     1381 cctgatggca tcaggcgaag tggtgatcca gtcgatgtgg agcccagccg tgaccgctgt
     1441 gcgcaccaaa ggaattgcct gcaattttca gcccctgaaa gaaggctacc gcgcctgggc
     1501 tgccggtttt gggctgccca ccaccttgtc gggtcgcaaa ctcgatggtg cctacgaatt
     1561 catcaactgg ttcctggacg gctgggctgg tgcttacctg aaccgccaag gctattacag
     1621 cgccgtgctg gagaccgcca agtcgaagat ggaagcctac gagtgggcct actggatgga
     1681 aggcaaggct gccacgcaag acatcaagag ccccaacggc gacgtgctgg ccaaaaccgg
     1741 tgcgacccgc gacggcggca gctacgaggc ccgcatgggc ggcatcgcct gctggaacgc
     1801 ggtgatggat gagaacaact acatggtcca gaaatggaac gagtttgtcg cagcttgatg
     1861 agaaattggc ctcaagccct tatgcaataa gcgctgacag ctactatatt tatagtatag
     1921 aagcaggtgc ttagcagttt tggggtcagg tcccgattcg gttcatgcct tgatggtcag
     1981 gcgctgatcc taccgaatgg ggcactgtcc ccaattctgc ggaacatggc agtgcttggt
     2041 ccctgccgct ggccagattc gcaatgacag tcaacccaca agagaacccc aatgaacacc
     2101 agagcggcat caccacctgc agcggcaccg gtcaccggtc gcggcattgc tgcgtggtgg
     2161 caggcgacac cgctcacgct ggtgtttgtg ctgttcttcc tgattccctt gggattgatc
     2221 ggcatggtta gtttctggga cttcaatgaa tacgaactgc tgcccgactt caccgtcaaa
     2281 cactatctgg cgatctttga gggttgcagc aacactgacg agatgtgcgt caccttcaag
     2341 acctatttgt ccaccttcaa gttcagtttc ctggtctggc tgatcacgct gctgattggt
     2401 tttatcgtgt cctacttttt agcgtttcat gtgcgctcca gcaatgtgca aacgctgctg
     2461 tttgtgctgt gcacgattcc gttttggacc agcaatgtga tccgcatgat ttcctgggtg
     2521 ccgctcctgg gtcgcaacgg gttggtgaac caaagcctgg tgggcgctgg tctgatcgac
     2581 acgcccattg aatggctgct gttttctgat ttttcagtgg tgctggcctt tgtgcacctg
     2641 tacaccatgt tcatgatcgt gccgattttc aacagcatga tgcgcattga ccgcagcctg
     2701 attgaggcgg ccaatgacag tggtgccagc gcctggcaaa cgctgtggca tgtgatcgtg
     2761 ccgttgtgcc gcaccggcat catcattggc tcgatctttg tcatcacgat tgtgatgggc
     2821 gactttgtca cgattggtgt catgggtggc cagcagattg cttcggtcgg caagatcatc
     2881 caggtgcaaa cgtcttattt gcagtttccg ctggcggcgg ccaatgcggt gatcttgctg
     2941 ctgctggtgc tgatgatcat ctgggcactg acgcgcctgg ttgacattcg taaggagctg
     3001 tgaatgagca acgacaagcg cagcgctggc ttctggtttc tggccctgtt ttttgcggcg
     3061 ttcgtgctgt ttttgtatgg cccgatgctg gtcattgtgg tgctgagctt tcaggggcca
     3121 gagggcgggc tgacctttcc cctgcggggc ctgtcgctgc actggtttgg caagctggcc
     3181 gaaggcctgg gcgtggtgga cattggcgcg gcgctcaagc gctcgctcgg cctggggctg
     3241 gcggtgatgc tgttgaccgt gctgttttcg gtgctggctg gcttggcgtt tcgcaagaag
     3301 ctcgccggtg gcaatgccct gttttatgtg gtggtggcca gcctgatcat gccgtcgatc
     3361 attgtgtcgc tgggtatcgg gctggaattc aggctgctcg acactggcct gaagtgggcg
     3421 ctggagttcc tgggcatgac gagcacatca gagagttatg gcacctcgct tgggttatac
     3481 acctcggccc ttggggccca cctgacctgg acgctgccgt ttgggctttt gatcatgttt
     3541 gcggtgttca accgctttaa cccggccttt gaggaagctg cacgggacct gggtgccaca
     3601 ccctggcagg ccttgcggca tgtggtgttg ccgctgatcg cgccctcggt ggttggcatt
     3661 ggcatgtttg gttttacctt gagctgggac gaaatcgccc gcacctcgca ggcaattggc
     3721 gatgtcaaca ctttgccgct tgagttacaa ggcctgacca ccaccgtaac cacaccgtcc
     3781 atttatgcct tgggcacggt gaccacggtc atatcattgg gggttatggt gctggccatc
     3841 ggtctggccc gctggttgag caaacggcaa caacaaggaa aacaatgagt gagacagcag
     3901 cggccccccc gctgacggat ggggacatgt acgagcgggt gatctcggcc atcctggacc
     3961 atcgcctgcc gccgggcacc aagctggtcg aggacaagct ggccagcgcc tttggtgtgt
     4021 cgcgcacccg aattcgcccg gtgctggtgc gcctggccaa cgagcaggtg gtcacgctca
     4081 cgcccaaccg gggtgccacg attgcccagc ccaccgagca ggaggcgcgt gaggtgtttc
     4141 aggtgcgcag cctgattgag ccgtcattgg ttgaactgtt catcgcccgg gccactgaga
     4201 ccgacatgaa caacctgaaa acctgtattg acgacgagga ggctgcgcgc aaggcgggcc
     4261 acatgcggcg ggccattcgc ctgtcaggac aattccacac attgattgcc gaaggggcca
     4321 atcacctgac gctggcgcgc atcctgcgcg agatgacttc gcgaaccacg ctgatcctga
     4381 tgacttacag cgcggcccat gaatttgagc gcaccgatgc cacgtcttgc ggatgtgatg
     4441 aacatcgtgc gctgctggat gcgatccggc tgcgtgaccc gcttgaagca gcgcggcgca
     4501 tgcgagcgca cttggcgcgg ctgcaggcgc agcttcggtt tgacgtgacg caagtggacc
     4561 tgccagacct gcacgccctg tttggcacgg cctcctagtc gttcgtttca tcacaaatga
     4621 cgacacgatg aagccgatgg attattcagt ctggccagac cagacgaggc gacatggccg
     4681 cagcctgtgc caggacgaac aacgccaggc caaaaaaagc gcgatgtcat gcaagcggtt
     4741 tggtcaaccg gacgcctagg cagcatgcgc cagttgctgg tcatcaaccc caacacgtcg
     4801 cccggcgtga ccgcgctgct gcgccagcac gtgcagcaag ccgccggttc acacgtggtg
     4861 gtgactgcag tgacggcccg ttttggcgca ccctatattg ccgatgaagc cagctatgcg
     4921 gtggctggtc atgccacgct ggatgcctgg gccgcagcac tggcgcaagc cgcacagccg
     4981 cctgatgcgg tgctgatcgg ctgttttgga gaccccgggc tgatggccct gcgtgagagc
     5041 agcccgacgc ccgtgaccgg cttggctgag gctgcgttca ttgaagctgc gcgccatggc
     5101 cggtttgcca tcgtcaccgg tggtgagcgc tgggcaccca ttctgcagcg gctggcccag
     5161 tcgctgggcc atgccagcgg gctggctggc attcacaccg tggcaccaac gggtgcgcag
     5221 ttggcggctg acccgattgc tgcacgtcgg ctgctgaccc aggcttgcct ggacgcggtg
     5281 cgtcagctcg gcgtgcaaac cgtgattttg ggcggtgccg ggctggccgg catggcagcc
     5341 ggcatacaac cctttgtgaa tgtgccgatt gttgacagcg taacggctgg cacccattgg
     5401 gccttgcgca gccatgtcac gccgccccag cgcgccacac ctggctttga cgtgccttgg
     5461 gtcaatctgt cggcagaact cacgggcatg agcctgcgct gataccaggc cgaaagcgac
     5521 tgaatcatcc atgtcgttgc tatctcgcct ggccttgcgt ttgcattgtc tgcggcgcga
     5581 gaacgcagac agtgctacat cacggcaagg cgaatcagca acctgtcgga tggatctggc
     5641 aatccaaaga catggtcaat tgtgggaaag gcgtggtgat gacaagcatg ccgcccgaat
     5701 ggcctgaaaa caatagcagg ctacgcatac aggacggggg ctacaggcca atttgacaca
     5761 atgctggggc gcatcggcgc gatggcacgg ctgataccct gcagccaacc gcatcaacgg
     5821 caggtgattt gttgcgtaat ttatgcccaa tcagcctcta gcccccgtcc tgcatgcgtg
     5881 aatagctata attaagatag cgttttgagc ttcaagtcga ccatctcgcg gctggctatg
     5941 ctgagatcgc agcggatctg gcgaaccggc tcgccgcagt tgcagtgctt ggtcggcgtg
     6001 gcgctcggcc ttgatctgcg gctgggtgcc aagacacgca ggatttagaa aaggagttgc
     6061 atgatcgaaa gcaacagcgt ccgccttttt tcgtgaactg accccaatcg cctcggcaaa
     6121 gttgccgaca atgccagctc caccccaagc aacactcacg aagggccgcc ccatgctttc
     6181 gaatgtcatg aatgcgctac agttgttcct gattccgctg ctgatctgcc tggtggtggc
     6241 agcggcagtg gtcatgttgg tgaggcggcg gcgcagcccg gcagcagcac ctgaccggtc
     6301 aaaccttgcc gacccgccaa ccgacgacga cagcgacttc ctggacgttg cgccgccacc
     6361 cccaccgggt gccgtag
//
LOCUS       DAOSLL010000224         6349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11091_length_6349_cov_4.316810, whole genome shotgun sequence.
ACCESSION   DAOSLL010000224 DAOSLL010000000
VERSION     DAOSLL010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6349
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11091_length_6349_cov_4.316810"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PLB25_10780"
     CDS             <1..901
                     /locus_tag="PLB25_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42097.1"
                     /translation="KGLLKKKSLGEFITVPIEDEDGQCGFRRLKVNSNSTNTWAVDEL
                     TGEKYELSDGDLCRYTVDKSYLRERLDQLLKLHLVDIPLTVDDQEPYLLGNYRMGDQR
                     LPVALVSRIWEPKHADKLDLQLRQSNLGLTIVLATTTGSPRRFLGPGIVVSLETLAQE
                     VEGQVCIDLSRVEGEIRRRQAAAVTTDTPTLIKEDARNALLIGPWPEPWSLTTKEWVD
                     VVEVLVNASSSQKRKCTKRQLEDAAGKSIRSMNEFFRGAPEWATYIRGADGNSKSRLW
                     ELNIGLTDYRQVSESTSVEAQSA"
     gene            1008..1271
                     /locus_tag="PLB25_10785"
     CDS             1008..1271
                     /locus_tag="PLB25_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010961853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HOE42098.1"
                     /translation="MQRQVSSVQPNRNAFRSPPNSAVRLALDENELAARWGLSVKTLR
                     RWRQEKLGPIHLKLGSRVTYLIHQIEAYERRVSRHSTSSAVYQ"
     gene            1284..1766
                     /locus_tag="PLB25_10790"
     CDS             1284..1766
                     /locus_tag="PLB25_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019717189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42099.1"
                     /translation="MSDLTLFPSDIAEMSVSQLAQLTPAQKLEVDVNLDKAIAWLKQA
                     RTKFDAALKQCYEEQAKAALLESGRDFGTAHINDGSLRIKFELPKKITWDQKQLGEIA
                     ERVVASGEQVKSYIDIKLSVSESRYTNWPPALQQQFSTARTVEPGKPTFTLSLDPLES
                     "
     gene            1769..2524
                     /locus_tag="PLB25_10795"
     CDS             1769..2524
                     /locus_tag="PLB25_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HOE42100.1"
                     /translation="MSQIIPFEFESHALRVNLDAAGQPWFVAADVCAALELPETHKAI
                     ARLDDDEKGRNSIPTPGGQQEMSVVNESGLYNLVLGSRKPEAKRFKRWITHEVLPSIR
                     KTGSYTSTTSVAALPSPTQDRVSSLLLIGEAVARVPGVKHGIAMAATLTCIFENTGLS
                     METMRRALPANDEPMAAMNPTKLGQQMGLAARSVNARLAAMGLQLRNDRDEWELTEAG
                     QAWGEALPYSRNGHSGYQILWNPAVIDQMKEVA"
     gene            2525..3370
                     /locus_tag="PLB25_10800"
     CDS             2525..3370
                     /locus_tag="PLB25_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE42101.1"
                     /translation="MALPIITADQRRAQRRGVKIVILGVSGIGKTTQLKSLDTHSTLF
                     IDLEAGDLSVSDWNGDCLRPRTWPEFRDLVVYLAGPNPALPEQSPFSQAHFDHVCSVY
                     GDPASLDKYQTYFCDSITALSRLCFNWAKSQPAAVSDRTGKPDSRGAYGLLGQEMITA
                     LTHLQHARGKNVVFVAILDSKTDDFGRKVFVPQIEGSATALQLPGIVDEVVTLAEIKA
                     EDGSSYRAFVTQTINPYSYPAKDRSGRLDLLEPPDLGALIAKCAGTGTPAQHPQAPTP
                     TDSKE"
     gene            3376..4038
                     /locus_tag="PLB25_10805"
     CDS             3376..4038
                     /locus_tag="PLB25_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003270457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42102.1"
                     /translation="MNDNNMTANAWSDFNDAEAQQSGFNLIPKGVLVPVLMTLKPGGH
                     YDASQGWSDGYPTQSSKTGAVYLAAEFVITGGEYAKRKMWSNIGLYSPKGPTWTQMGR
                     TFVRAALNSARNFLPQDNGPQAVAARRIQGFVDLDGLEFVVRVDIEKDDRGDDRNVVK
                     MAVEPDHPDYARTMGVPSKLTVNAYVSGSAAPYSAPVQTNAAAAPAPQRAPVSGKPAW
                     AQ"
     gene            4051..4326
                     /locus_tag="PLB25_10810"
     CDS             4051..4326
                     /locus_tag="PLB25_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42103.1"
                     /translation="MNASLPTAQAYHPGCFSDASQYQQWRTYAIKTRAGDSDYCTDCT
                     RAYQHQMIKQCRCNHAKTRFFVDCDGYTEGRRPVSERLVNCKKKGRR"
     gene            4323..4811
                     /locus_tag="PLB25_10815"
     CDS             4323..4811
                     /locus_tag="PLB25_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6511 domain-containing protein"
                     /protein_id="HOE42104.1"
                     /translation="MKCWVCSRQARGYGHTDNRHRTGQAQRYPLDWVFCSERCQKAFH
                     AMYGNWVRLKDDVVNAKGVAMVNLSEVEQNAMVKCLHAFGDAAGAIGFKKPLGDYSES
                     EALAVIDAIVTCFTQAMVEHHEKSKYPPVRGLPEVPDPMANLVANPFADMENDLPWED
                     AK"
     gene            4808..5545
                     /locus_tag="PLB25_10820"
     CDS             4808..5545
                     /locus_tag="PLB25_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009277338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42105.1"
                     /translation="MMDFNSSSSVSGQISTLIDLGLQRTRSKEKSRQYLGASRLGVSC
                     ERALQYEYAQAPVDPGRETLGRILRIFERGHVNEDSMVAWLRAAGFDLRTHKPNGEQF
                     GFSTADGRLQGHIDGVFVGGPEGFAYPALWENKCLGSKSWRDLEKNKLAVSKPVYAAQ
                     VAIYQAYLELHETPAIFTAVNADTMDIYAELVPFDAALAQRMSDRGVKVIAATEAGEL
                     LPRAYLDATHFECKFCAWQDRCWRTTP"
     gene            5542..5805
                     /locus_tag="PLB25_10825"
     CDS             5542..5805
                     /locus_tag="PLB25_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42106.1"
                     /translation="MNTPKQEFQMDAEPMIDAKQAACALRLPLYWFGDPKMRAKHRIP
                     HYLLGGLVRFRMNELSTWAANSSAAGDSDAEPQESEGASHDGL"
     gene            5792..>6349
                     /locus_tag="PLB25_10830"
     CDS             5792..>6349
                     /locus_tag="PLB25_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011003117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42107.1"
                     /translation="MMDFNDVTPAPSPSSDGNREEIRASLLLRLESVLKDMFPAGKVK
                     RGKFLVGDILGSPGDSLEIVVTGDKAGLWTDRATGQGGDIFDLISGHLALNVHSDFAK
                     VLNFAAQMVGKAPPEATRKRKAEPAIDELGPATAKWEYHDGEGKLIAIVYRYDPPGQK
                     KEFRPWDVKRKKATPPDPRPLYNQPG"
BASE COUNT         1431 a         1831 c         1800 g         1287 t
ORIGIN      
        1 caaggggctg ctcaagaaaa aatctctcgg tgaattcatc accgtgccca ttgaggatga
       61 ggatgggcag tgtggctttc gacgcctgaa ggtgaattcc aacagcacca acacctgggc
      121 ggtggacgaa ctcacaggtg aaaaatatga gctttcagat ggcgatttgt gccgttatac
      181 ggttgacaaa tcttatctgc gcgagcggct tgatcaactg ctcaaattgc accttgtcga
      241 cattcccctg actgtcgacg atcaagagcc atatttgcta ggcaactatc gcatgggcga
      301 tcagcgcctg ccagtcgcgc tggtttcgcg catctgggag cccaagcatg ccgacaagct
      361 ggacttgcaa cttcgtcaat ccaatcttgg attgactatc gtccttgcaa ccacgacggg
      421 atcgcctcgc cgattccttg gaccaggcat cgtcgtgtct ttggaaacct tggcgcaaga
      481 ggtggaaggt caggtctgta ttgatttgag ccgggtcgag ggtgagatac gtcgcaggca
      541 ggctgcggct gtaactactg atacgccaac gctcatcaag gaagacgcgc ggaatgcatt
      601 gctcattgga ccttggccgg agccctggtc gctgacaact aaagaatggg tcgatgtagt
      661 ggaagtgctg gtgaacgcat cgtcgagcca aaagcgcaaa tgcaccaaac gccagcttga
      721 ggatgctgct ggaaagtcaa ttcgttcaat gaacgagttt ttcaggggtg cacccgaatg
      781 ggctacctat attcgaggcg ctgacggcaa cagtaaatca cgactatggg agctcaacat
      841 cggattgact gattaccggc aagtttctga atctacaagc gttgaagcgc aatcagcgta
      901 aggattcagt cgttgtaatt tcaacgtgat ttctgtgtaa agactgcgag atatcgaagt
      961 ctgatctgcg cggaaatagg ggcaccccaa ctaaaggagt gctccaaatg caacgccaag
     1021 tctcttctgt tcaacccaac cggaatgcct tccggtcccc accaaacagt gctgtccgtc
     1081 tggcactcga cgaaaacgaa ctcgccgctc ggtggggcct ctccgtcaaa acgctgcgcc
     1141 gttggcggca agaaaaactc ggacccatac acctcaagtt gggctcaaga gtcacttatt
     1201 tgattcacca gatcgaagct tatgagcggc gcgtttcacg ccactcgact tctagcgctg
     1261 tgtatcaata aggagggaaa gtcatgtctg accttaccct tttcccctcc gacattgctg
     1321 agatgtccgt gagccaactg gcccagctga cacccgctca aaaacttgaa gtggatgtca
     1381 atctggacaa agccattgcc tggctcaaac aggcccgtac caagttcgat gccgccttga
     1441 agcagtgcta cgaagaacag gccaaagccg cgttgctgga atccggccgt gattttggca
     1501 ccgcccacat caacgacgga tccttacgca tcaagtttga gcttcccaaa aagatcacct
     1561 gggaccagaa gcaactgggc gagatcgctg agcgcgtcgt ggcatctggc gagcaagtca
     1621 agagctacat cgacatcaag ttgtcggttt ccgaatctcg ctacacgaac tggccaccgg
     1681 cgttgcagca gcagttttcc actgcccgca cggttgagcc gggcaaacca actttcaccc
     1741 tttctcttga cccactggag tcctgatcat gtcccaaatc atcccatttg aatttgaaag
     1801 tcatgccctg cgcgtcaacc tggatgctgc tgggcagccc tggtttgtcg ccgccgacgt
     1861 atgcgcagca cttgaactgc cagaaaccca caaagccatt gcccgtttgg atgatgacga
     1921 aaagggccgg aattcaattc cgacccctgg tggccagcaa gagatgtcgg tggtcaatga
     1981 gtccggcctc tacaacctgg tgttgggtag ccgcaagccc gaggccaagc ggttcaagcg
     2041 ctggatcacc catgaagttc tgccatccat tcgcaagact ggctcataca cgtcaaccac
     2101 gtcggtggca gcattgccat cgccaaccca ggatcgggtg tcatcactgc tgttaatcgg
     2161 agaagcggtg gccagggttc ccggtgtcaa gcatggcatc gccatggctg ccaccttgac
     2221 ctgcatcttt gagaacaccg gcctttccat ggaaaccatg cgtcgtgcgc tgcctgcgaa
     2281 tgacgagcca atggcagcca tgaacccgac caagctgggc cagcaaatgg gattggctgc
     2341 ccgcagcgtc aatgcccgtc tggctgcaat gggcttgcaa ctgcgcaatg accgtgacga
     2401 atgggagttg accgaagcag gtcaggcgtg gggcgaggcc ttgccgtact cgcgcaatgg
     2461 ccactcgggt taccagattc tctggaatcc ggcggtgatc gatcagatga aagaggtggc
     2521 ttaaatggcg cttcccatca tcactgctga tcagcgtcgc gcccagcgcc gtggtgtcaa
     2581 gatcgtcatt ctgggtgtga gtggcatcgg taaaacgacc cagctgaaat ccctcgatac
     2641 ccattccacc ttgttcattg atttggaggc gggtgacctg tcggtctctg attggaatgg
     2701 tgattgtctg cggccgcgca cctggccaga gttcagggat ctggtggtct atctggctgg
     2761 acccaacccg gcgctgcccg agcaatcacc tttttcgcag gcgcacttcg atcatgtctg
     2821 ctcggtttat ggcgacccgg ccagtcttga caagtatcag acctacttct gcgactccat
     2881 cacggcactc tcgcggctgt gctttaactg ggccaagagc cagcctgcag cagtttcaga
     2941 tcgcactggc aagcccgact cacgcggtgc ttatggtctt ttgggccagg aaatgatcac
     3001 ggcgctcacc cacttgcaac atgcccgggg caagaacgtc gtgtttgtgg ccatcctgga
     3061 cagcaaaacc gatgactttg gtcgcaaggt gttcgtgccg caaattgaag gcagtgccac
     3121 tgcactgcaa ctgccgggaa tcgtcgatga ggtggtgacg ctggcagaaa tcaaggccga
     3181 agatggcagc tcataccggg catttgtcac ccagaccatc aacccctaca gctatccggc
     3241 caaagaccgc agcggtcgtc ttgacctgct ggagccgccc gacctgggtg cgcttatcgc
     3301 caagtgcgct ggcaccggca cacctgcaca gcacccacaa gccccaaccc ccactgattc
     3361 caaggagtaa ttcaaatgaa cgacaacaac atgacagcca acgcgtggtc tgacttcaac
     3421 gatgccgaag cccaacagtc ggggttcaac ctgattccca agggtgtgct cgtgccggtg
     3481 ctgatgactc tcaaacccgg tggccactat gacgcaagtc agggctggtc ggacggatac
     3541 cccacccagt cttccaaaac gggggcggtt tatctggcag ccgagtttgt catcaccggt
     3601 ggggaatacg ccaagcgcaa gatgtggtcg aacattggct tgtactcgcc caagggtcca
     3661 acctggacgc agatgggcag aaccttcgtg cgcgccgcac tgaacagtgc cagaaacttc
     3721 ctgccgcagg acaacggccc ccaagccgtc gccgcacggc gcatccaagg ctttgttgac
     3781 cttgatggcc ttgagtttgt ggtgcgcgtg gacatcgaaa aggatgaccg gggtgacgac
     3841 cgcaacgtgg tcaagatggc cgtggagccc gaccacccgg actacgcacg caccatgggt
     3901 gtgccctcca agttgaccgt caacgcgtat gtgtcaggta gtgccgctcc atattcagca
     3961 ccagtgcaga ccaacgcagc ggcagcgcca gcaccgcagc gcgcgcctgt ctccggcaaa
     4021 cccgcatggg ctcagtaagg agcgccagcc atgaatgcct ctttacccac ggcgcaggcc
     4081 taccacccgg gctgcttcag tgatgcgtcg cagtaccaac agtggcgcac ctacgccatc
     4141 aagacccgcg ctggtgacag cgactactgc actgattgca cccgtgccta ccagcaccag
     4201 atgatcaagc agtgccgctg caaccatgcc aaaacccgct tctttgttga ctgcgacggt
     4261 tataccgagg gtcgtcgccc ggtcagtgaa cgtcttgtca attgcaagaa gaaaggcagg
     4321 cgatgaaatg ctgggtctgc tcacgtcaag cccgggggta tggtcatacc gacaaccggc
     4381 atcgcacagg acaggcccag cggtatccgc tggactgggt cttttgttcc gaacgctgtc
     4441 aaaaagcgtt tcacgccatg tatggcaact gggttcgttt gaaagacgac gtcgtcaatg
     4501 ccaaaggggt cgccatggtc aatctctctg aagttgagca aaacgccatg gtcaagtgcc
     4561 tgcatgcctt cggcgacgca gccggagcca ttgggttcaa aaagccactg ggtgattact
     4621 ctgaatctga agccttggcg gtgatcgacg ccatcgtgac ctgttttacg caggccatgg
     4681 tggagcacca cgagaagtcc aagtacccac cggtgcgtgg tctgccagag gttccggatc
     4741 ccatggcgaa tttggtcgcc aatccgtttg ccgacatgga aaacgacctg ccctgggagg
     4801 atgccaaatg atggacttca actcatcatc gagcgtcagt ggtcagatca gcaccctgat
     4861 cgatctgggt ctgcaaagga cccgttccaa ggagaaatcc cgccagtatc tgggagcatc
     4921 ccgtctgggc gtgtcgtgcg agcgcgcact gcaatacgag tacgcccaag cgccagtgga
     4981 cccggggcgg gagacgctgg ggcggattct gcgcattttt gagcgtggcc acgtcaacga
     5041 agacagcatg gtcgcgtggc tgcgtgccgc cggtttcgat ttgcgcacgc acaaacccaa
     5101 tggcgagcag tttgggtttt caacggctga tggtcgcttg caggggcaca tcgatggtgt
     5161 ctttgtcggc gggcccgaag ggtttgccta tccggcgctt tgggaaaaca agtgtctagg
     5221 ttccaaatcc tggcgcgacc tggagaaaaa caagctagca gtctcgaagc cggtctacgc
     5281 ggcgcaagtg gccatctacc aggcctactt ggagttgcat gaaaccccgg ccatcttcac
     5341 tgcggtcaac gccgacacga tggacatcta cgccgagttg gtgccgttcg atgcggcgct
     5401 ggcccagcgc atgtctgacc ggggtgtgaa ggtgattgcc gccaccgaag caggtgagtt
     5461 gctacctcgc gcctaccttg atgccaccca ctttgaatgc aagttttgcg cgtggcaaga
     5521 ccgctgctgg aggacaaccc catgaacaca ccaaaacaag aattccaaat ggatgccgag
     5581 cccatgatcg atgccaagca ggccgcgtgc gcgctgcgtc tgcccctgta ctggtttggc
     5641 gaccccaaga tgcgcgccaa acaccgcatt ccgcactacc tgctgggtgg tttggttcgt
     5701 tttcgtatga atgaactgag cacctgggct gccaacagca gcgccgctgg cgattccgac
     5761 gccgagcccc aagagtcgga gggtgccagc catgatggac tttaacgatg tgacaccggc
     5821 accatcaccg tccagcgatg gcaaccgcga agaaattcgc gccagcttgc tgctgcggct
     5881 tgagtcggtg ctgaaggaca tgtttcccgc tggcaaggtc aagcggggca agttcctggt
     5941 cggcgacatc ttgggcagcc cgggcgacag tctggagatt gtggtcaccg gcgataaggc
     6001 cggtctctgg acagatcgtg caactggtca gggtggagac atcttcgacc tgatttccgg
     6061 ccacttggcg ctcaatgtcc attccgactt tgccaaggtg ctgaactttg cagcgcaaat
     6121 ggtaggcaag gcaccaccag aggcgacgcg caaacgcaag gctgagcccg ccattgatga
     6181 actgggccca gcaacggcca aatgggagta tcacgacggc gaaggcaagt tgatcgcaat
     6241 cgtgtaccgc tatgacccac ccgggcagaa gaaggaattc cggccatggg atgtcaaacg
     6301 caagaaggct acaccgcctg atccacggcc actgtacaac cagccgggc
//
LOCUS       DAOSLL010000225         6346 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11096_length_6346_cov_4.413289, whole genome shotgun sequence.
ACCESSION   DAOSLL010000225 DAOSLL010000000
VERSION     DAOSLL010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6346
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11096_length_6346_cov_4.413289"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="PLB25_10835"
     CDS             complement(<1..916)
                     /locus_tag="PLB25_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HOE42108.1"
                     /translation="MKSKTTLALLDPALIGPAVRAAFAKLSPQAQWRNPVMFVVYVGS
                     ILTTLLGLQALQARGEAPADFIFAVAVWLWFTVLFANFAESLAEGRAQAQAQSLRSLK
                     KSTWAKKFQSPTNKLFHGSAWLPEQAEFLRKGDVLLVEAGDMIALDGEVIEGVASVNE
                     SAITGESAPVVRESGGDFSAVTGGTQVLSDWLVVRVTVNPGESFLDRMISMVEGAKRQ
                     KTPNEIALSILLVALTIVFLVVTVTLLPFSLFSVQAAGAGTAVSLTALVALLVCLIPT
                     TIGGLLSAVGVAGMSRMMQANVIATSGRA"
     gene            complement(948..2747)
                     /gene="kdpA"
                     /locus_tag="PLB25_10840"
     CDS             complement(948..2747)
                     /gene="kdpA"
                     /locus_tag="PLB25_10840"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HOE42109.1"
                     /translation="MSTSSWGLLALFLTTLLLGAWPLGRWLANLSSGHLPAWLLRAEA
                     PLYRLAGTTAGEPMHWSHYALALLAFNAIGVLAVYALQRLQIWLPLNPAGMAGISPDS
                     AFNTAVSFVTNTNWQGYAGESTMSYLTQMLALAVQNFFSAATGIAVAFALMRGFAARS
                     TSVIGNFWVDVTRITVWLLLPLSLVFAVFLAGQGVIQNFDAYKDVTTLEVTRFQQPKL
                     GPDGQVLKDDQGNPVMEETGTDKQTLAMGPVASQEAIKMIGTNGGGFFNANSAHPFEN
                     PTPLSNLLQMLAIFLIPAALCFAFGREVGDLRQGWAVLAAMTVLFVLAVMVITPAEQA
                     GNPLIGALGVDQNASSLQAGGNMEGKEVRFGITASSLFAVITTAASCGAVNAMHDSFT
                     PLGGMVPMVLMQLGEVVFGGVGSGLYGMLIFAMLAVFIAGLMIGRTPEYLGKKIEAHE
                     MKLTSIAILVTPLLVLVGSAIAVLAGSGQAGVANPGAHGFSEILYALSSAANNNGSAF
                     AGLSANTPFYNLLLGLAVWLGRFGVIVPVLAIAGALAAKKRLAVTGGTMPTHGPLFVM
                     LLMGTVLLVGLLNYVPALALGPMVEHLMLWSAR"
     gene            complement(2747..2845)
                     /gene="kdpF"
                     /locus_tag="PLB25_10845"
     CDS             complement(2747..2845)
                     /gene="kdpF"
                     /locus_tag="PLB25_10845"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HOE42110.1"
                     /translation="MISLGVLYGFAGTCALALLVYLVYALLRAEEF"
     gene            complement(3682..6282)
                     /locus_tag="PLB25_10850"
     CDS             complement(3682..6282)
                     /locus_tag="PLB25_10850"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HOE42111.1"
                     /translation="MAKQDEAAPQPDIAALRTQFRDGKKALIAHFMQSRATAPAATRL
                     IKALTRHVDATLAQLWTDCGMPASTALVAVGGFGRGELLPHSDVDVLLLLPDEATVET
                     GSPLKGTIEAFITACWDIGLEVGSSVRTVAECVLEARADQTVQTALLESRLVCGARKL
                     ALYFQRETSALMDAKAFLRAKTLEMRQRHTKYEDTPYALEPNCKESPGGLRDLQVVMW
                     VARAAGLGRNWAELAANGLITAFEVKQLQRNEGTLRLIRARLHAVAGRREDRLVFDLQ
                     TAVAESFGYTSSRSARSSEVLMHRYYWAAKAVTQLNQILMLNIEERINGSEAAPMRQI
                     NARFFDRAGMLEVARDSLYAENPHAILETFLVFQQTPGIKGLSARTLRALYNARDQMD
                     TAFRHDPINRELFMDILRQPQGITHAFRLLNQTSVLGRYLWVFRRIVGRMQHDLFHVY
                     TVDQHILMVLRNVRRFFMVEHAHEYPFCSQLAADWDKPWILYVAALFHDIAKGRGGDH
                     SALGTLEVRRFCQQHDITGEDARLVEFLVREHLTMSRIAQKSDLSDPDVIANFAKRVG
                     SEHALTALYLFTVADIRGTSPKVWNAWKGRLLEDLYRATSRALGGHVPDLHAEVQERQ
                     RDALIELALRTQPFESHKALWATLDVGYFMRHDAVEIAWHTRMLSRHPGTREPIVRGR
                     RSPVGEGLQVLVYTPDQEDLFARICGFFDRSGFSILDARIHTSQNGYALDTFQVITTT
                     LTEHYRELISMIEVGLSRVIAAAQPLPPPSRGRVSRRVKSFPITPRVSLKPDEKAQRW
                     LLNISASDRVGLLYSVARVLAKHKINLQLAKITTLGERVEDTFLIQGAALQHTRDQIE
                     IETELLDALA"
BASE COUNT         1250 a         1977 c         2006 g         1113 t
ORIGIN      
        1 ccgcgcggcc cgaggtggca atcacattgg cctgcatcat gcggctcatg ccggccaccc
       61 cgaccgccga caacaggccg ccgatggtgg tcggaatcag gcacaccagc agcgccacca
      121 gtgccgtcag gctgacggcg gtaccggccc cggcggcctg aacactgaac agcgagaacg
      181 gcagcagcgt gacggtgacc accagaaaca cgatggtcag cgccaccagc aagatcgaca
      241 gcgcgatctc gttgggggtt ttctggcgtt tggcaccctc aaccatgctg atcatgcggt
      301 ccagaaagga ctcgcccgga ttgaccgtga cgcgcaccac cagccagtct gacagcacct
      361 gggtgccacc ggtgacagca gaaaaatcac cgcccgattc acgcaccact ggcgcagatt
      421 cgccggtaat ggcgctttcg ttgaccgacg cgacaccttc aatgacctcg ccatcgagtg
      481 caatcatgtc gcccgcttcg accagcagca catcgccttt gcgcaggaac tcggcctgtt
      541 cgggcagcca ggcgctgcca tgaaagagtt tgttcgtggg gctctgaaac ttcttggccc
      601 aggtgctttt tttcagactg cgcaatgatt gcgcctgcgc ttgcgcccgg ccttcggcca
      661 gactttcggc aaagttggca aacagcacgg taaaccacag ccagacggcc acggcaaaga
      721 tgaagtcggc cggtgcctcg ccccgggcct gcagtgcctg cagacccagc aaggtggtca
      781 gaatgctgcc gacatagacg acaaacatca ccgggttacg ccactgggct tgcgggctca
      841 gtttggcaaa ggccgcccgc acggcaggcc caatcagcgc cgggtcaagc agcgccaggg
      901 tagttttgct tttcatggga tgaactcgct aatttgatgt tgacggttta ccgggctgac
      961 cacagcatca ggtgctcaac cattggcccc agcgccaggg ccggcacgta gttaagcagg
     1021 ccgaccagca gtaccgtgcc catcagcagc atgacgaaca gcgggccatg ggtgggcatg
     1081 gtgccgccgg tgacggccag gcgctttttg gcagccagcg caccggcaat ggccagcact
     1141 ggcacgatca cgccaaagcg ccccagccat accgccagcc ccaacagcag gttgtaaaac
     1201 ggggtgttgg ccgacaggcc ggcaaaggcg ctgccgttgt tgttggcagc cgaactcaag
     1261 gcataaagaa tttcagaaaa accatgggcg ccgggattgg ccacaccagc ctggccgcta
     1321 cctgccagca cggcgatggc gctgccaacc agcaccagca gcggggttac cagaatggca
     1381 atggaggtca gcttcatttc atgtgcttcg atcttcttgc cgaggtactc cggcgtgcgg
     1441 ccgatcatca agccggcaat gaacaccgcc agcatggcaa agatcaacat gccataaagg
     1501 ccgctgccaa ccccgccaaa caccacttcg ccgagctgca tcagcaccat cggcaccatg
     1561 ccgcccagcg gcgtgaagga gtcgtgcatg gcattgaccg cgccacagga ggcagcggtg
     1621 gtgatcacgg caaacaggct cgatgcagtg atgccaaagc ggacttcctt gccctccatg
     1681 ttgccgcccg cttgcagact gctggcattt tggtccaccc caagggcccc gatcagcggg
     1741 ttgccagcct gctcggcagg cgtgatgacc atgacggcca gcacaaacag cacggtcatc
     1801 gccgccagca cggcccagcc ctggcgcagg tcaccaacct cgcgtccaaa ggcaaagcac
     1861 agggccgccg gaatcaggaa aatggccagc atttgcaata ggttgcttaa aggcgtgggg
     1921 ttttcaaacg ggtgggccga gttggcgttg aaaaagccgc caccgttggt gccaatcatc
     1981 ttgatggctt cttgcgaggc gaccgggccc atcgccaggg tctgtttgtc ggtcccggtt
     2041 tcttccatga ccggattacc ttggtcgtct ttcagtacct ggccatcagg gccaagcttg
     2101 ggctgctgga agcgggtgac ctccagcgtc gtcacgtcct tgtaggcatc aaagttttga
     2161 atcacgccct ggcctgccaa aaagacggca aacaccagcg acaggggcaa tagcaaccat
     2221 accgtgatcc gcgtcacatc aacccagaag ttgccgatga cactggttga acgcgcggca
     2281 aagccacgca ttagcgcaaa ggccacggca atgccggtgg cagcagagaa aaagttctgc
     2341 accgccagcg ccagcatttg agtcaggtaa ctcatggttg actcacccgc atagccctgc
     2401 cagttggtgt tggtgacaaa gctgacggcg gtgttgaaag ccgagtcagg cgagatacca
     2461 gccatgccag ccggattgag tggcagccag atttgcagcc gctgcagggc ataaaccgcc
     2521 aatacgccaa tggcgttaaa ggccagcagt gccagcgcat agtgcgacca gtgcatcggt
     2581 tcgccagccg ttgtgcccgc cagccggtac agcggggcct cggcgcgcag cagccaggcg
     2641 ggcagatggc cagagctcaa gttggccagc caccggccaa gcggccaggc acccagcagc
     2701 aaggtggtca gaaaaagggc cagcaagccc catgacgatg tgctcatcag aactcctcgg
     2761 cgcgcagcag cgcatagacc agatagacca gcaaggccag ggcgcatgta ccagcaaaac
     2821 cgtaaagaac gcccaggctg atcatgtccg ctcccctttg gcctgctcca gccgcacgaa
     2881 gcctcgtacc agcaatacca tcaagcccca cagggcggcg gcaacgccga gatagaccag
     2941 atccatgttc actcctttgt cattgagatg agagaagtgt gctcagcgct gtataaaaac
     3001 ggggttgagt tttgccaggc ggctgtaaaa agagtgtaaa aaacgcggcc gacttcaggg
     3061 cgtttgatgg ggtcggcaag cgggcagcgg gtttgggtcg tggcgcggtc ggcccaacgg
     3121 ggactttgct gtagtcacaa tagctataaa atatgtagct gctagcgcat atctggcaag
     3181 ggctacagcc ccaaaatgtt taaacctgga gcgctggccc aacttctcaa agtctgcagc
     3241 cagtcagtga aatgactggc agcaaatgac ttcgatggct ttcacccgct gagcgacagt
     3301 gcgacgcacc ggtttgacgc accggcggcg cgcctgggcg cgttgctcat ccttcgcccg
     3361 ccagggcggc atgcaaagtc attcgccgcg agggcgcgac tcctacaaat tcaaatgggc
     3421 gccgactgtt tgtgtaggag tcgcgccctc gcgacgaagg ctcatcgatt ccggctcgtc
     3481 cggcttaggt tcaagagcct tttgcaccgc cgccgcaagc tgcgtgcggc ggctgtgcag
     3541 ggcacagcca tgccagaggc tgggcctgct gcgaccgctc agacttgcgc aggcacgttt
     3601 gaagccacgg ctttggtcta gccagaaact gacaccagag gcccgcgcgg gcgctttcgc
     3661 tgaggaaacc tgatgaaaaa atcaggccag tgcgtcgagc agctcggtct cgatctcgat
     3721 ctggtcgcgg gtgtgttgca gcgccgcgcc ctgaatcagg aaggtgtctt cgacccgctc
     3781 gccgagcgtg gtgattttgg ccagttgcag gttgatcttg tgtttggcca acacccgcgc
     3841 cacactgtag agcagcccga cccggtcact ggccgaaatg ttgagcagcc agcgctgggc
     3901 tttttcatcg ggtttgaggc tcacccgcgg tgtgatcgga aaactcttga cacgtcttga
     3961 cacccgcccg cggcttggtg gcggcagcgg ttgtgcagcg gcaatcaccc ggctcaagcc
     4021 cacctcgatc atgctgatga gttcccggta gtgctcggtc agggtggtgg tgatgacctg
     4081 gaaggtgtcg agcgcatagc cgttttggct ggtgtggatg cgggcatcca gaatgctgaa
     4141 gccgctgcgg tcaaaaaagc cgcagatgcg ggcaaacagg tcttcctggt cgggcgtata
     4201 gaccagcacc tgcaagccct caccgactgg cgagcggcgt ccccgcacga tcggttcgcg
     4261 cgttcccgga tggcgcgaca gcatgcgcgt gtgccaggca atttccactg cgtcgtggcg
     4321 catgaagtag ccgacatcga gggtcgccca aagcgccttg tgcgactcga acggctgcgt
     4381 gcgcagcgcc agttcgatca gcgcatcacg ctgacgttct tgcacttcag catgcagatc
     4441 gggcacatgc ccgcccaggg cgcgtgaggt ggcgcggtac aggtcttcca gcaaacggcc
     4501 tttccaggcg ttccagactt ttgggctggt gccgcgaata tcggccacgg tgaacaggta
     4561 gagcgccgtc agggcgtgtt cgctgccgac ccgcttggca aaattggcga tcacatcggg
     4621 gtcgctcaag tcggattttt gcgcaatgcg gctcatcgtc aggtgctcgc gcaccagaaa
     4681 ctcgaccagc cgggcatctt cgccggtgat gtcgtgctgc tggcaaaaac gccgcacttc
     4741 caatgtgccc agcgccgaat ggtcaccgcc acggcctttg gcaatgtcgt gaaacagcgc
     4801 ggccacgtac agaatccagg gcttgtccca gtcggccgcc aattgtgagc aaaacgggta
     4861 ctcgtgggca tgctcgacca tgaagaagcg ccgcacattg cgcagcacca tcaggatgtg
     4921 ctggtcgacc gtgtagacat ggaacaggtc gtgctgcatg cgcccgacga tgcggcggaa
     4981 cacccacagg tagcgcccga gcaccgaagt ctggttgagc agcctgaacg cgtgcgtgat
     5041 gccctgcggc tggcgcagga tgtccatgaa cagttcgcgg ttgatcgggt cgtgccggaa
     5101 ggccgtgtcc atctggtcgc gcgcgttgta gagcgcccgc aaggtgcgcg ccgacagccc
     5161 cttgatgccg ggcgtctgct ggaagaccag aaaggtctcg aggatggcgt gcgggttctc
     5221 ggcgtagagg ctgtcgcgtg ccacttccag catgcccgcg cgatcgaaga agcgcgcgtt
     5281 gatctgccgc atcggtgcgg cttcgctgcc gttgatgcgc tcctcgatgt tgagcatcag
     5341 gatctggttg agctgggtca ccgccttggc cgcccagtag tagcggtgca tcagcacctc
     5401 ggacgagcgc gcgctgcggc tgctggtgta gccgaagctt tcagccacgg ccgtctgcag
     5461 gtcgaagacc aggcggtctt cgcgccggcc ggccaccgca tgcagccggg cgcggatcag
     5521 ccgcaaggtg ccttcgttgc gctgcagttg cttcacttca aaggctgtga tcaggccgtt
     5581 ggcggccagc tcggcccagt tgcggcccag cccggcggca cgcgccaccc acatcaccac
     5641 ctgcaggtcg cgcaggccgc ccgggctttc cttgcagttg ggctccagcg cgtagggcgt
     5701 gtcctcgtac ttggtgtggc gctggcgcat ttccagcgtc ttggcccgca ggaaggcctt
     5761 ggcgtccatc agcgccgagg tttcacgctg aaagtacaaa gccagcttgc gcgcgccgca
     5821 caccaggcgc gattcgagca gcgcggtctg cacggtctgg tcggcacgcg cctcgagcac
     5881 gcattcggcc acggtgcgca ccgacgagcc gacctccagc ccgatgtccc agcaggcggt
     5941 gatgaaggcc tcgatggtgc ccttgagcgg gctgccggtt tcgaccgtgg cctcatcggg
     6001 caacagcagc agcacgtcca catcgctgtg gggcagcaac tcgccacggc cgaagccacc
     6061 caccgccacc agggccgtgc tggccggcat gccgcaatcc gtccacagct gggccagggt
     6121 ggcgtcgaca tggcgggtca gggccttgat cagccgggtg gccgccgggg cggtggcgcg
     6181 gctttgcatg aagtgcgcga tcagcgcctt cttgccatcg cggaactgcg tgcgcaacgc
     6241 ggcgatgtcg ggctgcgggg cggcttcgtc ctgcttggcc acggcggcgc ccggggtcag
     6301 gccgcggtga tgaaggccgg cggcgggggg ctgccggccg acaggg
//
LOCUS       DAOSLL010000226         6328 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11144_length_6328_cov_4.792125, whole genome shotgun sequence.
ACCESSION   DAOSLL010000226 DAOSLL010000000
VERSION     DAOSLL010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11144_length_6328_cov_4.792125"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="PLB25_10855"
     CDS             <1..294
                     /locus_tag="PLB25_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HOE42112.1"
                     /translation="ADANPYVALAMTLACGYLGLKNKIKPKPEMKGDAYLSPYSLPRS
                     LGEALDWLRRESDLHEVLGKEFITVYSEIKELEFEEFMKVISPWEREHLLLHV"
     gene            356..1738
                     /locus_tag="PLB25_10860"
     CDS             356..1738
                     /locus_tag="PLB25_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017453503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HOE42113.1"
                     /translation="MITKDIQTLDSAHFIHPFTDHGELARRGARVITRAEGIYVWDSE
                     GEKMLDAMSGLWCVNVGYGRKELAQAAYQQMMTLPFYNSFFQTTNVPAVKLAAKLAAL
                     APKVDGRSFEHVFYSSSGSESNDSNVRMVRRYWDLLGQPQRKVIISRHNAYHGSTMAG
                     ASLGGMSGMHAQGDLPIPNITHIGQPYFFEGGKQGESADEFGLRAAGWLEEKILQLGA
                     DKVAAFIAEPIQGAGGVIIPPATYWPEIQRIVDKYGILLISDEVICAFGRLGHWFAYE
                     KFGYKPDLVTFAKAVTSGYIPLGGVMVGDRVAKVLIEKGGEFNHGYTYSGHPVACAVA
                     LANLEIMETEQLPQRVRGEVGAYFAKRYAELNDHPLVGMTESCGFVAGLVLVKDKHTK
                     VRFRQEDAVGMVCRHHCFTNGLIMRAVGDRMIIAPPLVLTHAEIDELLRLIRLALDLT
                     QVELKARGLL"
     gene            1845..3017
                     /locus_tag="PLB25_10865"
     CDS             1845..3017
                     /locus_tag="PLB25_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE42114.1"
                     /translation="MKKYLATVALSALLLAACGKKEEPPAPVAAPAAVASAAASAPAP
                     APAEEKVLNIYNWPDYVPAGMIAAFEKETGIKVNYDTFETNEALHAKLVAGNTGYDIV
                     VPGSVFAKPQIEGGMFQPLDKAKIKNLANLDPAIMGTLAKNTDPGNKHLVPWAWGFTT
                     VGINKTKVTKALGKMPMPENAWDLVFKPEYTSKLKSCGIAYLDSPTEIIPVALHYIGK
                     DAYSNDPADYKAAADMLAKVRKDVRLFSSTMIDDIAGGKACVVIGWSGDINIAAGRAK
                     ENKSKDEIESLLPSTGALIFFDTMAITKDAKHPNNASVFIDFYLRAENAALMTNEMNY
                     PTANKAAIEKIKPEMVGNKTIFVEADYFAKMIAPSSFSNEAREAMATAYNAFKKGK"
     gene            3179..3671
                     /locus_tag="PLB25_10870"
                     /pseudo
     CDS             3179..3671
                     /locus_tag="PLB25_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121976.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     assembly_gap    3672..3681
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3682..4208
                     /locus_tag="PLB25_10875"
     CDS             <3682..4208
                     /locus_tag="PLB25_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HOE42115.1"
                     /translation="CVMVTHDQEEAMTMAGRIAVMSKGRVLQVGSPQEVYEYPGSRFV
                     ADFIGSVNLLDGKLSVDETDHCAALTAIGEVHVGHSISGTLGMPVAVAVRPEKVSIGK
                     KAPQGLSHNLFNGRVKEIAYFGSYNTYIVVTPQGYRMKITEPNGSHLDLLDITWEDDV
                     FFWWDDTDALVLRD"
     gene            4227..5147
                     /locus_tag="PLB25_10880"
     CDS             4227..5147
                     /locus_tag="PLB25_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE42116.1"
                     /translation="MTKLSLKVPGRGFVIGLPYTWLVVFFLLPFLILLYISFVDMGND
                     ISPFKPIWDSSTGLLTLKYENYWTIFRSDDGSGGLFQTIYIEAYVRSLLYALATAVLC
                     LLIGYPFSYFVARSKPSVRPALLMMVMLPFWTSFLLRVYAWKGILADQGVINQVLMSL
                     GLVDEPIQMLYTNVSMLVGMTYVYLPFMVLPLYANLVKMDFRLLEAAYDLGTSPLKAF
                     WLVTVPLSKAGIIAGFMLVFIPCVGEFVIPSLLGGPENIMIGRVVWDEMFTSNNWPRA
                     SALAVVMIGLIVIPLAVYYHYTGDAAEPRH"
     gene            5158..6018
                     /locus_tag="PLB25_10885"
     CDS             5158..6018
                     /locus_tag="PLB25_10885"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE42117.1"
                     /translation="MNNFLIRYFGKAWIALTFLFLYLPLMFMVVFSFNSTRQDAQFTG
                     FSLRWYQALTQDTKIVEGFWLSLQVATASGIASAVLATFAAFVLVRYRRFPGRTLFSG
                     MVNAPLVMPEVVIGLSLLLLMVGIQNAFGWPQRGMLTIVLGHTLLGMAYGMVVVQSRL
                     LEMNRAIEEAAMDLGAKPFQVFFLITMPNIFQAILAAYLLAFTLSFDDVVISEFLSGP
                     GVNTLPQVIFGYARRGINPTIYAAATLLIVSVTVVVIAYSVWVARQTRKRELEIAAAT
                     RAEIAAIQPK"
BASE COUNT         1305 a         1772 c         1854 g         1387 t
ORIGIN      
        1 gccgatgcca atccctatgt ggcgctggcc atgacgctgg cctgcggcta tctgggcttg
       61 aagaacaaaa tcaagcccaa acccgaaatg aagggcgacg cctacttgtc gccttattcg
      121 ctgccgcggt ctctgggtga ggcgctggac tggttgcgcc gcgaatccga cctgcatgag
      181 gtgctgggca aggagttcat caccgtctat tccgagatca aggaacttga attcgaagag
      241 ttcatgaaag tgatctcgcc ttgggaacgg gagcatttgc tattacatgt atagctgcca
      301 gcgcatgact gacaagggct agagcctcat ttcgtatgta aaaaggacgc gattcatgat
      361 taccaaagac atccagaccc tcgacagtgc ccactttatc cacccgttca ccgaccacgg
      421 cgaactggcc cgccggggcg cgcgggtgat cactcgggct gagggcattt atgtctggga
      481 ctccgagggc gaaaaaatgc tcgatgccat gagcgggctg tggtgcgtca atgtgggtta
      541 tggccgcaag gagcttgcgc aagcggccta ccagcaaatg atgacgctgc cgttttacaa
      601 cagttttttc caaaccacca atgtgcccgc agtcaagctg gcggccaaac tggccgcgct
      661 ggcacccaag gtggatggtc gcagtttcga gcatgtgttt tattcaagca gcggctccga
      721 gagcaacgac tccaacgtgc gcatggtgcg ccgctattgg gacctgctgg gccagccgca
      781 gcgcaaggtc atcatcagcc gccataacgc ctaccacggc agcaccatgg ccggggcttc
      841 gctgggcggc atgagcggca tgcacgcgca gggtgacttg ccgattccga acatcacaca
      901 cattggtcaa ccctactttt ttgagggcgg caagcaaggc gaatcggcag atgaattcgg
      961 tctcagggcg gcgggctggc tggaggaaaa aattctgcaa ctcggtgccg acaaggtggc
     1021 ggcgttcatt gccgagccga tccagggtgc cggcggggtg atcattccgc ccgccactta
     1081 ttggcccgag atccagcgca ttgtggacaa atacggcatc ttgctgatca gtgacgaggt
     1141 aatttgcgcc tttggccggc tgggccactg gtttgcgtat gaaaaatttg gctacaagcc
     1201 tgacttggtg acctttgcca aggcggtcac cagtggttac atcccgctgg gtggcgtgat
     1261 ggtgggtgac cgcgtggcca aggtgctgat tgagaagggc ggcgagttca accacggcta
     1321 cacctacagt gggcacccgg tggcttgtgc cgtggcgctg gccaacctgg agatcatgga
     1381 gaccgaacaa ttgccgcagc gcgtgcgcgg cgaggtgggg gcgtacttcg ccaaacgcta
     1441 tgccgagctc aacgatcatc cgctggttgg catgacggag tcgtgcggct ttgtggctgg
     1501 cttggtgctg gtgaaggaca aacataccaa agtacggttc aggcaagaag acgcggtggg
     1561 catggtgtgt cggcatcatt gttttaccaa cgggctgatc atgcgcgctg ttggtgaccg
     1621 catgatcatc gccccgccgc tggttctgac ccatgccgag atcgacgagt tgctgcgcct
     1681 gattcggctg gcgctcgatc tgacgcaagt cgagctcaaa gcccggggtt tgctctgagc
     1741 attctgggca acaattatgt ggtctgattt ttgctagact ttaaactagt cggtttctgt
     1801 tctccccttg tgttttcttg tcctttgatg gaggttgttt catgatgaaa aagtatcttg
     1861 cgactgttgc gctgtcggcg ctgttgttgg ccgcgtgcgg caaaaaagaa gagccgcccg
     1921 cacctgtggc ggcccccgct gcagttgcca gcgctgctgc ttcagcgcca gcaccggcac
     1981 cggcagagga aaaggtgctc aacatctaca actggcctga ctacgttccc gctggcatga
     2041 tcgccgcgtt tgagaaagag accggcatca aggtcaatta cgatactttc gagaccaatg
     2101 aagccctgca cgccaagctg gtggcgggca atacgggtta cgacatcgtg gtgccgggct
     2161 cggtgtttgc caagccacag atcgagggcg gcatgttcca gccgctggac aaggccaaga
     2221 tcaagaacct ggccaacctg gatccggcca tcatgggcac gctggccaaa aacaccgacc
     2281 ccggcaacaa gcatctggtg ccgtgggcct ggggctttac caccgttggc atcaacaaga
     2341 ccaaggtgac caaggcgctg ggcaagatgc ccatgcccga gaacgcctgg gacctggtgt
     2401 tcaaacccga atacaccagc aaactgaaat cttgcggcat tgcctacctg gactcgccaa
     2461 ccgaaatcat cccggtggcg ctgcattaca tcggcaagga tgcctattcc aacgacccgg
     2521 ctgactacaa ggcggcggcc gacatgctgg ccaaggtacg caaggacgtg cgcctgttca
     2581 gctcgaccat gatcgacgac attgcgggcg gcaaggcctg cgtcgtcatt ggctggtcag
     2641 gtgacatcaa catcgccgct ggccgtgcca aagagaacaa gtccaaggac gagatcgaat
     2701 ccttgctgcc gtcaaccggg gcactgattt tctttgacac catggccatc accaaagatg
     2761 ccaagcaccc caacaatgcg tctgtgttca ttgattttta cctgcgtgcc gaaaatgccg
     2821 cgctgatgac caatgaaatg aactacccca ccgccaacaa ggccgccatc gagaagatca
     2881 agcctgaaat ggttggcaac aagacgatct ttgttgaagc cgactacttt gccaagatga
     2941 ttgcgccttc gagcttcagc aacgaagcgc gcgaagccat ggcgacggcc tataacgcct
     3001 tcaaaaaggg caagtaaggt acttttgaag tagcacaagg ctgtttgtac aagggtacaa
     3061 acagcctttt tctttggcaa caagacggcg caagcccaag gtgggaccta tggctgaaca
     3121 agattcccgg caagactatc tggtgaccga agaactggtc aagcgctttg acgaggcgct
     3181 ggcggtcgac gaggtcaacc tgtcaatcaa gcagggcgag atttttgcct tgctgggcag
     3241 ttccggctgc ggcaaatcca ccttgctgcg gatgctggcg gggttcgaga agcctacttc
     3301 ggggcgcatc ctgctaagcg gtcgggacgt ggccaatctg gcaccctacg agcggccgtt
     3361 caacatgatg tttcagagct acgcgctttt tccgcatctc gacatctggg agaacgtggc
     3421 ttttggctta aggcgcgaag gtttgcccag cgccgatatc aagcagcgcg tgggcgaaat
     3481 gctcgatctg gtgcaactcg gcccctatgc ccggcgcaag ccgcaccagt tgtcgggtgg
     3541 ccaacagcaa cgcgtggcgt tggcgcgcag cctggccaag cgcccgaagg tgctgctgct
     3601 cgatgagcca cttggcgcgc tggacaaaaa gctgcgcgaa caaacccagt ttgaactggt
     3661 caacatcgtc gnnnnnnnnn ncctgtgtca tggtgacgca cgaccaggaa gaagccatga
     3721 cgatggcggg tcgcattgcg gtgatgagca aaggccgggt gctgcaggta ggctcgcccc
     3781 aagaggtgta tgagtatccg ggcagccgct ttgtggccga tttcattggc agtgtgaact
     3841 tgctcgacgg caagctcagt gtggacgaaa cggaccactg cgccgcgcta accgcgattg
     3901 gtgaagttca tgtgggccac agcatcagtg gcaccctggg catgccggtg gccgtcgccg
     3961 tgcgccctga aaaggtgagc atcggcaaaa aggcaccgca aggcctgagt cacaacctgt
     4021 tcaacggacg ggtcaaggaa atcgcctatt ttggttcgta caacacctac atcgttgtca
     4081 cgccccaggg ctaccggatg aaaatcaccg agccgaatgg ttcacacctg gatttgctcg
     4141 acatcacctg ggaagatgac gtatttttct ggtgggacga caccgatgcc ctggtgttgc
     4201 gcgactgatc aaccgaggcc attgcaatga ccaagctttc actgaaggta cccggcagag
     4261 ggtttgtgat cgggttgccc tacacctggc tggtggtgtt ttttctgctg ccgttcctga
     4321 ttttgttgta catcagcttt gttgacatgg gcaacgacat ttcgccgttc aagccgatct
     4381 gggattccag caccgggttg ctcacgctca agtacgaaaa ctactggacg attttccgct
     4441 ctgatgacgg cagcggcggg ctgtttcaga ccatttacat tgaggcctat gtgcgctcgc
     4501 tgctgtatgc attggccact gccgtgttat gcctgctgat tggctacccg ttttcgtatt
     4561 tcgttgcgcg tagcaagccc agtgtgcgac cggccctgct gatgatggtg atgctgccgt
     4621 tctggacatc ttttctgttg cgtgtttatg cctggaaggg cattctggct gaccagggcg
     4681 tgatcaatca ggtgctgatg agcctgggcc ttgtcgatga gccgatccag atgctctaca
     4741 ccaatgtctc gatgctggtc ggcatgacct atgtctacct gccgttcatg gtgctgccgc
     4801 tgtacgccaa cctggtcaag atggatttcc ggctgcttga agctgcttac gacttgggca
     4861 cttctcccct caaggcgttc tggctggtca cggtgccgct gtccaaggct ggcatcattg
     4921 ccggattcat gctggtgttt atcccttgcg tcggtgagtt tgtgattcca tcgctgctgg
     4981 gcggaccgga gaacatcatg atcggccggg tggtgtggga cgaaatgttc accagcaaca
     5041 actggccacg tgcttctgcc ctggcagtgg tgatgatcgg gctgatcgtc attccgctgg
     5101 cggtctatta ccactacacc ggcgacgcgg ctgagccccg tcactaagga tcgcagcatg
     5161 aacaatttcc tgatccgata ctttggcaag gcctggatag cactgacctt tttgtttctg
     5221 tacctgccgc tgatgttcat ggttgtgttc tctttcaaca gcacccggca agacgcgcag
     5281 ttcaccggtt tttcgctgcg ctggtaccag gcgctcacgc aggacaccaa gatcgttgaa
     5341 ggcttctggt tgtcgctgca ggtcgcgact gcctctggca ttgcgtctgc ggtgctggcc
     5401 acttttgccg cttttgtgct ggtgcgctat cggcgttttc ctgggcgcac cttgttttcc
     5461 ggcatggtca acgcgccgct ggtaatgccc gaggtggtga tcggcctgtc cttgctgctg
     5521 ctgatggtgg gcattcaaaa tgcctttggc tggccacagc gcggcatgct gacgattgtg
     5581 ctgggccaca ccctgctggg catggcatac ggcatggtgg tggtgcagtc gcgattgctg
     5641 gagatgaacc gtgccattga agaggccgcg atggacctgg gggccaaacc gtttcaggtg
     5701 ttctttctga tcaccatgcc aaatatcttc caggccatat tggccgccta cctgctggcg
     5761 ttcaccctgt cgtttgacga tgtggtgatt tccgagttct tgtccggtcc tggtgtcaac
     5821 accttgccac aggtcatttt cggctatgcc cgccgcggca tcaacccgac catctacgcc
     5881 gcagccacct tgctgatcgt gtcggtgacc gtcgtggtaa ttgcctacag cgtctgggtc
     5941 gcccgccaga cccgcaagcg tgaacttgaa atcgccgccg ccacccgggc cgaaatcgct
     6001 gccattcagc ccaaatgagg cggtgaagtc aaaacatggt tacccaatgc agcaatgggt
     6061 cgcactgccg tagcagattt ggggtcggat cccaattcgg accaaaccgt ctggcattgt
     6121 tggcagcgcc aaaccgaatt gggctctgac cccaattctg cgggccaaag cgaaccatcc
     6181 ccaatccgac caaacaagca tcaacagaat caaaccaaca ttcaaaggca acacatgaac
     6241 cccaactacg acggccgtgc ctttatcaat ggcgagcgca cgccggccca aagccaacaa
     6301 accttcgact gccactcgcc cgtcgatg
//
LOCUS       DAOSLL010000227         6326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11148_length_6326_cov_4.914687, whole genome shotgun sequence.
ACCESSION   DAOSLL010000227 DAOSLL010000000
VERSION     DAOSLL010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11148_length_6326_cov_4.914687"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..3174)
                     /locus_tag="PLB25_10890"
     CDS             complement(151..3174)
                     /locus_tag="PLB25_10890"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HOE42118.1"
                     /translation="MKTLVIAEKPSVAQDIVRALTPLSGKFDKHDEHFENDNYVVSSA
                     VGHLLEIQAPEAYDVKRGKWSFAHLPVIPPHFELKPVDKTKTRLNAVVRLAKRKDVDQ
                     LINACDAGREGELIFRLIEQYAGGAKPLGKPVHRLWLQSMTPQAIRDGFGTLRSNVQM
                     QPLADAARCRSEADWLVGINGTRAMTAFNSRDGGFFLTTVGRVQTPTLSVVVEREELI
                     RKFVSRDYWEIHASFAAQAGEYPGKWFDPKFKRALADAAPGHEEVDAELKPDRLWNQR
                     EAQAIADAVRGQPASVTEESKPTTQASPLLFDLTSLQREANGKFGFSAKTTLSIAQSL
                     YERHKALTYPRTDSRALPEDYLPVVKKTFEMLAGSGMKHLAPHALTALNNSYITPTRR
                     VFDNTKVSDHFAIIPTLQAPSALSEAEQKIYDLVVRRFMAVFFPAAQYQVTTRISTVA
                     VASLGALSGSHNFKTEGKVLVKPGWLAIYGKEAAAEVADAKDGDKGQSLVPVAPGEIV
                     KTESAQAKGLKTRPPARYSEATLLGAMEGAGKTVEDDELREAMQEKGLGTPATRASII
                     EGLIAEKYMLREGRELIPTAKAFQLMTLLRGIGVEELSKAELTGEWEYKLNQMEQGKL
                     RRDTFMAEIAAMTERMVKKAKEYDRDSVPGDYATLATPCPNCGGIVKENYRRYTCTGA
                     DGTSDGCGFSFGKTPAGRTFEPTEVEQFLRDRKIGPLDGFRSKAGWPFTAEMVIKFDE
                     ESNNYKLEFDFGDDKKGEESGELVDFSDQQQLGPCPKCGAGVFEHGKNYLCQKSVPTE
                     GQPTPNCDFKSGQVILQQPIAREQMHKLLATGKTDLLDKFVSMRTRRAFKAMLVWDAA
                     AGKVNFEFAPSKFPPRKTAAVPLVSAQTASNTIAGSADGTRAGGIKLLKTATKTATRP
                     SVKKVAAKVAAPRKAAVAGVGSTPSAALAAVIGPAPVARAQVIKQLWDYIKANNLQDA
                     ANKRAINADAKLLAVFGKPQVSMFELAGIVGKHLG"
     gene            3277..3789
                     /locus_tag="PLB25_10895"
     CDS             3277..3789
                     /locus_tag="PLB25_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HOE42119.1"
                     /translation="MSKKPSASSRPIQVRRSGIHGRGVFAVLPIAAGLDIIEYVGEII
                     TWAQAQARHPHNPADPNHTFYFHVDEARVIDALAGGNASRWINHSCDGNCEAEQRAGR
                     IFIKALRDIAAGEELHYDYGLVIDERYTPKLKAEYPCWCGASNCRGTLLAPKRRRSTR
                     ARSDTKASGR"
     gene            3786..4589
                     /locus_tag="PLB25_10900"
     CDS             3786..4589
                     /locus_tag="PLB25_10900"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HOE42120.1"
                     /translation="MSQAAVMTWPVEAISQAITPLLPGFALELLPEVDSTNAELMRRA
                     QAGRCESCLLLAERQSAGRGRLGRPWHAGTSWSDSLTFSLGMPLAPKDWSGLSLAVGL
                     SLAQSLHPEIRIKWPNDLWWRQRKLAGILIETVTWSGLGASRYVVIGVGLNIVAPAVD
                     GLATDPAGLVELLPGIDAGQALLRLAQPLVQTIKRFEGQGFAPFQADFNARDALAQMA
                     VTLSDGLQGVAQGVDGAGALLVQTAQRVQRVISSEVSVRPLRGPSPGVG"
     gene            4602..5276
                     /locus_tag="PLB25_10905"
     CDS             4602..5276
                     /locus_tag="PLB25_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOE42121.1"
                     /translation="MLRLIVLMLVLLNAVYFAWGQGWLQTLGVGPTVTSEPQRLKQQI
                     RPQALVVIGDSAMAQKRASEAATGVGLCLQSGVLNEAQASAVRKVLVDGSWPSGSWTL
                     DGVALPAQWIIYMGRYDSAAELEKKRAQLEGMRLTLHPLANAALEPGLSLGVFTSEPE
                     ARAALELLAQRGVRTAKVLLNTPAPQQFRLRLPAIDGALQKQLEPVKSALADSPLVPC
                     QVGAQP"
     gene            complement(5296..>6326)
                     /locus_tag="PLB25_10910"
     CDS             complement(5296..>6326)
                     /locus_tag="PLB25_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE42122.1"
                     /translation="LGNGKPALVQVAETLEKRSVLATEIVKGVMLPQFVILPMAVLLV
                     WLALVQAIKPLSQLEERIRARKPDDLSPIDAQAVPLEVTPLVESVNDLLLRLMDSIAT
                     QKRFLADAAHQLKTPLAGLRMQADLAQREGASAEDLKHSLRQIGRASIRATHSVNQLL
                     ALARAESSGSAMPRMRCDLADLCMSVVRDCVPFALEKHIDLGYEGTQAGTPGCTILAN
                     PTLIKEMVRNLVDNAINYTPSSLENPGIITARVLTDPFGRALVLQVEDSGPGVPEAER
                     ELIFQPFYRALGTEADGSGLGLPIVLEIANQHNASVSLEDCHPGQNPPGTRFVVRFMA
                     SPADDTEA"
BASE COUNT         1127 a         1896 c         1979 g         1324 t
ORIGIN      
        1 cgcccaaatc cgccggtgca aataggggaa gttatttctg accacatcct aagcccaagt
       61 catgggtcga gaaaccggtg cgaaatgcgc cagcgcgacc ggatcacagc cgacgttcca
      121 gcagcccgac agactcccag gcgctaacgt tcagcccaga tgcttgccga caatgcctgc
      181 cagttcaaac atgctgacct gcggtttgcc aaataccgcc agcagcttgg catcggcatt
      241 gatggcgcgc ttgttggcag cgtcctgcag attgttggcc ttgatgtagt cccacagttg
      301 tttgatgacc tgggctcgcg ccaccggtgc cggcccaatg accgcagcca gcgcggcact
      361 gggcgtggag ccaacgccgg ccaccgccgc cttgcgcggt gcggccacct tggcagcgac
      421 ttttttgacg ctgggccgag tcgcggtctt tgtcgctgtc tttaagagtt ttatccctcc
      481 agcccttgtc ccatctgcgc taccagctat agtattagaa gcagtctgag cgctgaccag
      541 tggtacagcg gcggttttgc gtggcgggaa cttgctcggt gcaaactcaa aattgacctt
      601 gccagcagcc gcgtcccaga ccaacatggc cttgaaagcg cgccgggtgc gcatgctgac
      661 gaacttgtca agcagatcgg ttttgccggt ggccagcagc ttgtgcatct gctcgcgggc
      721 aatcggctgc tgcaggatga cctgaccact cttgaagtcg caatttggcg tgggctggcc
      781 ttcggtcggc accgatttct gacacaggta attcttgccg tgctcaaaaa cgcctgcgcc
      841 gcacttgggg caaggcccga gttgctgctg atctgaaaaa tcaacaagtt cgccggattc
      901 ctcacccttc ttgtcgtcgc cgaagtcgaa ttcaagcttg tagttgttgc tttcctcgtc
      961 aaacttgatg accatttcgg cggtgaacgg ccagcccgcc ttggagcgaa aaccatccag
     1021 tgggccaatt ttgcggtccc gcaggaattg ctcgacctcg gttggctcaa aggtgcgccc
     1081 ggccggtgtc ttgccaaacg aaaagccaca gccgtcgctg gtgccatcgg ctccggtgca
     1141 ggtgtagcgg cggtagtttt ccttgacaat gccgccgcag ttcggacagg gcgtggccag
     1201 tgtggcgtag tcgccgggca cgctgtcgcg gtcgtattcc ttggcttttt tcaccatgcg
     1261 ttcggtcatg gcggcaattt cggccatgaa agtgtcacgc ctgagtttgc cctgctccat
     1321 ctggttgagt ttgtattccc actcgccagt gagctcggct ttggacagct cctccacgcc
     1381 gataccgcgc agcagggtca tcaactggaa cgccttggcg gttggaatca gctcgcggcc
     1441 ttcgcgcagc atgtattttt cggcaatcag accttcgatg atggatgccc gggtggccgg
     1501 ggtgcccagg cctttttcct gcatggcttc acgcaattcg tcgtcttcga cggttttgcc
     1561 agcgccttcc atggccccga gcaaggtggc ttcggaatag cgggccggtg ggcgggtttt
     1621 cagacctttg gcctgcgcag actcggtttt gacgatttca ccgggcgcga ccggcaccag
     1681 gctctggccc ttgtcaccgt ctttggcatc agcgacttca gcggccgcct ctttgccgta
     1741 aatggccagc cagcccggct tgaccagcac cttgccttcg gtcttgaagt tgtggctacc
     1801 ggacagtgcg cccaagctcg ccactgcgac cgtgctgatg cgcgtcgtga cctggtattg
     1861 ggcagccgga aaaaacaccg ccatgaaacg gcgcaccacc aggtcgtaaa ttttctgctc
     1921 ggcttccgat agcgcactgg gcgcttgcag cgtcgggatg atggcaaagt ggtcactcac
     1981 cttggtgttg tcaaagaccc gccgggtcgg tgtgatgtaa ctgttgttga gcgccgtcag
     2041 ggcgtgcggt gccaggtgct tcatgccgga gccggccagc atctcgaagg tttttttcac
     2101 cactggcaga tagtcttccg gcaaggcgcg cgagtcggtg cgcgggtagg tcagggcctt
     2161 gtggcgctcg tacaggcttt gcgcaatgga cagcgtggtt ttggcgctga agccgaattt
     2221 cccattggct tcgcgctgca ggctggtcaa atcgaacagc agcggtgatg cctgggtggt
     2281 gggcttgctt tcttcagtca cgctggccgg ctggccacgc accgcatcag caatcgcctg
     2341 ggcttcgcgt tgattccaca gccgatcagg tttgagttcg gcatcaacct cttcatggcc
     2401 tggggctgcg tcagccagcg cacgcttgaa cttggggtca aaccatttgc cggggtactc
     2461 acccgcctgc gcggcaaagc tggcgtgaat ttcccaatag tcacggctga caaatttgcg
     2521 gatcagctct tcgcgctcga ccaccacgct caaggtcggt gtttgcaccc ggccgacggt
     2581 ggtcagaaaa aagccgccgt cgcgcgagtt gaatgccgtc atggcacggg tgccgttgat
     2641 gccgaccagc cagtcggcct cactgcggca gcgcgcggca tcggccagcg gctgcatctg
     2701 gacattgctg cgcagcgtgc caaagccgtc gcgaatcgcc tgcggtgtca tcgactgcag
     2761 ccacagccgg tggaccggtt tgcccagtgg cttggcacca ccggcatatt gttcgatcag
     2821 gcgaaagatc agttcgcctt cgcgcccggc atcgcaggca ttgatgagct ggtccacgtc
     2881 cttgcgtttg gccagcctga caaccgcgtt gagcctggtt ttggtcttgt caaccggttt
     2941 aagctcaaag tgcggaggaa tcaccggcag gtgggcaaag ctccatttgc cgcgtttcac
     3001 gtcataggct tcgggagcct ggatttccag caggtggccg acggcgcttg agaccacata
     3061 gttgtcgttc tcgaaatgct cgtcgtgctt gtcaaacttg cccgacaggg gtgtcaacgc
     3121 ccgcacgatg tcttgggcga cggaaggttt ttcggctatg accagggttt tcatctgact
     3181 acaatccatt tttctcgcgg gcgcctaagc gcacgcacac acaggcaggt acgcacatgc
     3241 gcacccatac gagttcacga taccaaattt tcaaccgtgt cgaaaaaacc atctgcaagc
     3301 tcccgtccga tccaggttcg ccgctccggc atccatggcc gaggggtgtt tgcggtgttg
     3361 ccgattgcag cgggtcttga catcatcgaa tacgtgggcg aaatcatcac ctgggcgcag
     3421 gcgcaggcgc gtcacccgca caacccggct gaccccaacc acacgtttta ctttcatgtt
     3481 gacgaggccc gggtgattga cgcgctggcc ggtggcaatg catcgcgctg gatcaaccat
     3541 tcgtgcgacg gcaattgcga ggccgagcag cgggccgggc gtatcttcat caaggcgctg
     3601 cgcgacattg ccgccgggga agaactgcac tacgactacg ggctggtcat tgatgaacgc
     3661 tacacgccca agctcaaggc cgagtacccc tgctggtgtg gtgccagcaa ctgccggggc
     3721 acgctgctgg cacccaagcg caggcgcagc acccgggcgc ggtcggacac caaggcgtcc
     3781 ggcagatgag ccaagccgcc gtgatgacct ggccggtaga ggcgatttcg caagcgatca
     3841 cgccactgct gcctggtttt gccctggaac tgttgccaga agtggattca accaatgccg
     3901 agctgatgcg tcgtgcccag gccgggcgct gtgaatcgtg tttgctgctg gccgagcgcc
     3961 agagcgccgg tcgcggtcga ctcgggcgac cctggcatgc gggcacgtcc tggtccgaca
     4021 gtctgacgtt ttcgctgggt atgccactgg ccccaaagga ctggtcaggt ttgtcgctgg
     4081 cggtgggctt gagtctggcg caaagcctgc acccggagat ccggatcaag tggcccaatg
     4141 atttgtggtg gcgccagcgc aagctcgcgg gtatcttgat tgaaaccgtg acctggagcg
     4201 gtttgggggc cagccgttat gtcgtgatcg gtgttggcct gaacattgtg gcaccagccg
     4261 tcgacgggct tgccaccgat cctgccgggc tggtcgagtt gctgcccggc atcgatgccg
     4321 ggcaggcgct gcttcgcctg gcccaaccgt tggtgcagac catcaagcgc tttgaagggc
     4381 agggttttgc accttttcag gcagatttca acgcccgtga tgcattggcg caaatggcgg
     4441 tcacgctcag cgatggattg cagggcgtgg cacagggcgt agatggcgcg ggggccttgc
     4501 tggtacagac ggcacagcgg gtgcagcggg tgatcagctc ggaagtcagc gtgcgaccgt
     4561 tgcgtggtcc cagtccaggt gttggttaag gttttattgc catgttgcgg ctgatcgtgc
     4621 tgatgctggt gctgctgaac gcggtttatt tcgcctgggg tcagggctgg ctgcaaacgc
     4681 ttggggtggg gccgaccgtc accagcgagc cgcaacggct caagcagcaa atcaggccgc
     4741 aagcgctggt cgtgattggc gacagcgcca tggcacaaaa gcgcgcctct gaggctgcga
     4801 ccggggtcgg cctgtgcctg caaagtggtg tgctcaacga ggcgcaggcc agcgccgttc
     4861 gcaaggtatt ggttgacggc tcctggccca gcggatcctg gacactcgac ggcgtggcgc
     4921 tacccgcgca gtggatcatc tacatgggca gatacgacag cgcggcagaa ctggaaaaga
     4981 agcgcgccca gttggaaggc atgcggctga ctcttcatcc tttggccaat gcggcactgg
     5041 agcccggtct gtcgctgggg gtgtttacct ccgagcctga ggctcgggcg gcgctggaat
     5101 tactggccca gcgcggcgtg cgtaccgcca aggtgttgct gaacacgcca gccccgcagc
     5161 agtttcggct gcgcttgccc gccatcgatg gggcactgca aaaacagttg gagccggtca
     5221 aaagtgcgct ggccgatagc cctctggtgc cctgccaggt tggcgcccag ccataggcac
     5281 tggcaaaaca ccggctcaag cctcggtgtc atctgccggg ctggccatga accgcaccac
     5341 aaaacgcgta cctggtgggt tttgacccgg atgacaatcc tccaggctga cgctggcatt
     5401 gtgctggttg gcaatctcca gcacgatcgg caggcccaat cctgagccgt cggcctcggt
     5461 gcccagcgca cggtaaaacg gctggaagat cagttcgcgt tccgcctccg gaacacctgg
     5521 cccggagtct tctacctgca gcaccagggc gcgcccaaac gggtcggtca acacacgagc
     5581 cgtgatgatg cccggatttt ccaggcttga cggggtgtag ttgatggcgt tgtcaaccag
     5641 attgcgcacc atttccttga tcaaggtggg gttggccaga atggtgcagc cgggggtacc
     5701 ggcctgcgtg ccttcatagc ccaggtcaat gtgtttttca agtgcaaacg gcacacagtc
     5761 gcgcaccacc gacatgcaca agtcggccag atcgcagcgc atgcgtggca tcgccgagcc
     5821 gctgctttcg gcgcgcgcca atgccagcaa ctggttcacg ctgtgggtgg cacggatgct
     5881 ggcacgacca atctggcgta gcgagtgctt caggtcttcg gcactggcac cctcgcgctg
     5941 cgccaggtcg gcctgcatgc gcaagccggc cagcggtgtt ttgagctggt gcgcggcatc
     6001 agccagaaag cgcttttgcg tggcaattga atccatcagg cgcagcaaca ggtcgttgac
     6061 cgattccacc agcggcgtga cttcaagcgg caccgcttgt gcgtcaatcg ggctcagatc
     6121 gtcaggcttg cgggcgcgaa tgcgctcttc aagctggctt agcggcttga tcgcctgcac
     6181 cagtgccagc cagaccagca gcaccgccat cggcagaatg acaaactgcg gcagcatcac
     6241 gcccttgacg atctcggtgg ccaacaccga gcgtttttcc agcgtttcgg ccacttgcac
     6301 cagcgctggt ttgccgttgc caagcg
//
LOCUS       DAOSLL010000228         6316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11165_length_6316_cov_4.649098, whole genome shotgun sequence.
ACCESSION   DAOSLL010000228 DAOSLL010000000
VERSION     DAOSLL010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6316
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11165_length_6316_cov_4.649098"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..1714)
                     /gene="pap"
                     /locus_tag="PLB25_10915"
     CDS             complement(83..1714)
                     /gene="pap"
                     /locus_tag="PLB25_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0043751 - polyphosphate:AMP
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate:AMP phosphotransferase"
                     /protein_id="HOE42123.1"
                     /translation="MFESAEIGHKLDKTAFREAVPGLRAALQEAQVDLFTKHKFPVVV
                     LISGQDGAGKGETINTLYEWMDPRFLSTLAFSEPSDEERERPPMWRYWRSLPPKGRIG
                     IFAGSWYSDPIRERIQGEISMKELDARAQQINRFEEMLVNEGALVLKFWFHLTKEGQR
                     TRLKALEADPRTAWRVTQWNWDRLKTYDQLQDVAGHLLRITNTAAAPWVVVEGSDDRY
                     RSLTVGQVLLDALRRRLASTDQQHALVAPMVRVNLDGRNVLSELDLTQVLDDKPYKDE
                     LAHWQGRLSELIRDLRFKNRSLVCAFEGADAAGKGGAIRRIAAALDARQYQVNPVAAP
                     TDEEKAQPHLWRFWRQLPRLGHVAIFDRTWYGRVLVERVEGFCSEADWLRAYSEINDF
                     EHELIDAGVIMVKFWLQISQEEQLRRFQEREKIDFKRFKITQEDWRNREKWEDYQMAI
                     CDMVERTSTGNAPWTLVEANDKNFARVKILRTLCERLELELSGRKADLPHVAAKTAEK
                     PTAKTDKSEKSLKSDKAAIRPAEKSGSHKSKASSK"
     gene            2061..3629
                     /locus_tag="PLB25_10920"
     CDS             2061..3629
                     /locus_tag="PLB25_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE42124.1"
                     /translation="MQATRYKIWVQLLATIAAALLVVWTGVIIWQGHVTRQAALDQAT
                     DFSLSMHNATMAGLTGMMVTGTVAQRDVFLDQVKQLGSIRDVRVLRGEAVIKLFGPGT
                     EKDDSKPDVLEQQVLQTGKAIVLVESDAKGEYLRAVRPTLALKNSLGKDCTSCHATPE
                     GTVLGAVSMKVSLDKVNASLADQRVKSILASLITCIPVLMLIYPFIRRVVTRPLEYCI
                     KVASGIAKGDLTQRIDVTSSNEMGQLQQALKDMSCSLEGIVREVRMGTDSIFSASSDI
                     ASGNIDLSNRTELQASSLESTAASMAEVTSAVNKNAEHARHANELAHSASDVAMRGGT
                     VVSGVIDTMGSINQSSKRIVDIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA
                     SEVRNLAKRSSDAAREIKALIGDSVSKVEAGSVLVQQAGRTMSEIVKSIQSVTDIMVE
                     ISVAETDQTSGIERVGSAIDRMNEVTQQNAALVEQAAAAAQSLQDQAEHLEHVVSTFK
                     VLGQNSTQTLIGRA"
     gene            3670..4899
                     /locus_tag="PLB25_10925"
     CDS             3670..4899
                     /locus_tag="PLB25_10925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE42125.1"
                     /translation="MPPSPLTFAVADAPTQAGFNSIRWLEADGAHAALWRSERGVAAP
                     SRVLLADDTITADSAYRLACEGTALLWRGDFQNARQLLQALARRADQLPKRKRKKAGQ
                     VLAPAEAFHLHRLAQSQRSRMLGMLLIEFNADYSIPLRRAPDFRVACAQAWGVADPGL
                     GCSVASLRELLGIGSAHEWRRVGVEIAALGAAPDNRIHPHYGVFSPVRGEYIQLLATA
                     HLPKPPSDQASLLAFDIGTGTGVLAAVLARRGIQRIIATDIDPRALACAQENLTWLGL
                     DKQVEVIKAELFPPGLASLIVCNPPWLPARPGSPLERAVYDEGGRMLNGFLQGLRAHL
                     APKGEGWLILSNLAEHLGLRTREALLALFADNGLRVISRIDARPVHPKASDPTDALHQ
                     ARAKEVTSLWRLAALPA"
     gene            5225..5842
                     /locus_tag="PLB25_10930"
     CDS             5225..5842
                     /locus_tag="PLB25_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOE42126.1"
                     /translation="MQTRLFPLKLASCLLTSSLVWPGAGALAQSAATVRQAAPASPAP
                     ASPACPPILKHSFNRLQDEAPQDLCQYAGKVILMVNTASYCGFTGQYEGLEALYAKCQ
                     ARGLVVLGFPSNDFGQQEPGSNKEIADFCFNTYAVKFPMFAKTVVSGPERNLLYAKLA
                     KESGTTPKWNFYKYLIDRSGRVVDSYNSTTSPQSKRLVAAIEKLL"
     gene            6198..>6316
                     /locus_tag="PLB25_10935"
     CDS             6198..>6316
                     /locus_tag="PLB25_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HOE42127.1"
                     /translation="MDKLRIDKWLWAARFFKSRSLASDEIDKGRVQVNGAAVKP"
BASE COUNT         1294 a         1913 c         1839 g         1270 t
ORIGIN      
        1 ctgtgcgccc aaatccgccg gtgcaaatag gggaagttat ttctgaccac atcctgagct
       61 tcattgacga agcttgcggc acttatttac tgctggcctt tgacttgtgg ctgcctgatt
      121 tttcggctgg ccggatggct gccttgtctg atttgagcga tttttccgac ttgtctgttt
      181 tggcagttgg tttttcggca gtctttgccg ctacatgtgg caaatccgct ttgcggccag
      241 acaactcaag ctcaagccgc tcgcacagcg tgcgcaaaat cttcacgcga gcgaagtttt
      301 tgtcgttggc ctcaaccagc gtccagggcg cattgccggt gctggtgcgt tcgaccatgt
      361 cgcagatggc catttgatag tcttcccatt tctcgcggtt tcgccagtct tcctgagtga
      421 tcttgaaacg cttgaaatcg attttttcgc gttcctgaaa gcgccggagt tgctcttcct
      481 ggctgatttg cagccagaac ttgaccatga tgacacccgc atcaatcagt tcgtgctcaa
      541 agtcattgat ctcgctgtag gcccgcagcc agtccgcttc cgagcaaaaa ccctcgaccc
      601 gctccaccag cacccggccg taccaggtgc ggtcgaatat ggcaacgtga ccaagcctgg
      661 gaagctggcg ccaaaaacgc cacaaatgag gctgcgcctt ttcctcatcg gtgggtgccg
      721 ccaccgggtt aacctggtat tgacgcgcgt cgagtgccgc agcaatgcgg cgtatggccc
      781 ctcccttgcc cgccgcgtcg gccccctcaa aagcgcatac cagtgaccgg tttttgaacc
      841 gcaggtcacg aatcagctcg gacaggcggc cctgccagtg ggccagttcg tccttgtagg
      901 gcttatcgtc aagcacttgc gtcaagtcca gttcggacag cacattgcgg ccatccaggt
      961 tgacccgcac catcggggcg accagcgcat gctgctgatc cgtacttgcc agccgacgcc
     1021 tgagcgcatc cagcaacacc tggcctaccg tcagcgagcg gtaacggtcg tcgctgcctt
     1081 caaccaccac ccagggcgct gcagccgtgt tggtgatgcg cagcaggtgc ccggccacgt
     1141 cctgcaactg atcgtaggtt ttgaggcgat cccagttcca ttgcgtgacc cgccaggcgg
     1201 ttcgcgggtc ggcctccagc gcctttagcc tggtgcgctg gccttccttg gtcaggtgaa
     1261 accagaattt caacaccagg gctccttcat tgaccagcat ctcttcaaaa cggttgatct
     1321 gctgggcgcg ggcatcgagc tctttcatcg atatctcgcc ctgaatgcgt tcgcgaatcg
     1381 gatccgaata ccatgacccg gcaaaaatgc caatgcgccc ttttggcggc agcgagcgcc
     1441 agtaacgcca catcggcggg cgttcgcgct cttcgtcact gggctcggaa aacgccaggg
     1501 tggacaaaaa cctggggtcc atccattcat acaaggtgtt gatggtctcg cccttgccgg
     1561 caccatcctg accgctgatc agtaccacca ccggaaactt gtgtttggtg aacaagtcaa
     1621 cctgcgcctc ctgcaacgcc gcacgcaaac cgggaaccgc ttcgcgaaaa gctgtcttgt
     1681 ccagtttgtg accgatttct gctgattcaa acatgggagc cgttcctgaa aatctgcact
     1741 gtaaacaaat gcgaagtcgc gttgcagact gcgtagtcta gtccagctca aaaccgattg
     1801 cgcctacaac aaagtcggat tattctgaga tactgcggtt gccaggcctg accgccgacc
     1861 gatcagtttc tggccgagcc gggcagccga cggatgtcgg catgacacct gagtagccaa
     1921 cttactggag ctcaatgttg cgcctcgacc ttttctgacg gccaattcct attggtacat
     1981 ctgctctagc gtcagcggcc cagttggtgt aagcaaagcg taaaatgccc gacgcgtcgt
     2041 tgaagataaa ggaatacgcc gtgcaagcta ccaggtacaa aatttgggtt cagttattgg
     2101 caacgatcgc agcggcattg ctggtggtgt ggacgggtgt catcatctgg caaggccatg
     2161 tcacccgaca ggcggcgctg gatcaggcaa ctgacttttc cctcagcatg cacaacgcca
     2221 ccatggccgg actgaccggc atgatggtca ccggcacggt agcccagcgc gatgtttttt
     2281 tggaccaggt caagcaactg gggtcgattc gggatgtgcg ggtgctgcgg ggcgaagcgg
     2341 ttatcaaact gtttgggccg ggcaccgaaa aagacgactc gaaacccgac gtgctggaac
     2401 aacaggtctt gcaaaccggc aaggcaattg tgctggttga gtccgatgcc aaaggcgagt
     2461 acctgcgcgc cgtgcgccca acgctggcgc tgaaaaactc gctgggcaag gactgcacca
     2521 gttgccacgc aacccctgaa ggcacggtgc ttggcgcggt cagcatgaag gtttccctcg
     2581 acaaagtcaa tgccagcctg gctgatcagc gcgtcaaatc gattctggca tcactcatca
     2641 cctgcattcc ggtgctgatg ctgatctatc cattcattcg ccgcgtcgtg acgcgcccgc
     2701 tggaatactg catcaaggtc gccagcggca ttgccaaggg tgatctgacg caaaggattg
     2761 atgtcacgtc gagcaacgag atgggccaat tgcagcaagc cctgaaagac atgagttgca
     2821 gccttgaggg catcgtgcgc gaggtgcgaa tgggcactga ttccattttc agtgcgtcaa
     2881 gcgatatcgc cagcggcaac atcgatttat ccaaccgcac cgagttgcag gccagttcgc
     2941 tcgagagtac ggcggcctcg atggccgagg tgacctcggc cgtcaacaaa aacgccgagc
     3001 atgcgcgcca cgccaacgag ttggcgcatt cagcttcgga tgtcgcaatg cgcgggggta
     3061 cggtggtgtc tggcgtgatt gacaccatgg ggtccatcaa ccagtcttcg aagcgcattg
     3121 tggacatcat tgcggtgatt gacggcatcg cttttcaaac caatatcctg gccctcaacg
     3181 cggcagtgga agcagcccgg gccggcgagc aaggccgagg ctttgccgtg gtggcatccg
     3241 aagtgcgcaa tctggccaag cgctcgtctg atgcggcgcg ggaaatcaag gccttgattg
     3301 gcgactctgt cagcaaagtc gaggcgggca gcgttctggt gcagcaagcg ggtcgcacca
     3361 tgagcgaaat tgtgaaaagc atccagagcg tgacggacat catggtcgag atttcggtcg
     3421 ccgagactga tcagaccagc ggcatcgaac gcgtgggcag cgccattgac cggatgaatg
     3481 aagtgacaca gcaaaacgcc gctttggtgg aacaggcggc cgccgcggcg cagtcgctgc
     3541 aagaccaggc cgaacacctc gagcatgtcg tcagcacctt caaggtattg ggtcaaaatt
     3601 cgactcaaac ccttattgga cgtgcgtaag aagctctgtt tataatagca ttttttatta
     3661 cccacgctga tgccaccttc gccccttacc ttcgccgtcg ctgatgcacc cacgcaagcg
     3721 gggttcaaca gcattcgctg gctggaggca gacggtgccc atgcagcctt atggcgatcg
     3781 gaacgaggcg ttgcagcacc aagccgggtg ctgctggctg acgacaccat caccgccgat
     3841 agcgcttatc gcctggcctg cgaaggcacg gcactgctgt ggcgcggcga tttccagaac
     3901 gcccgccaac tgctgcaggc gctggcacgc cgggctgacc agttgcccaa acgcaagcgc
     3961 aaaaaggccg ggcaagtgct tgccccggca gaggcttttc acctgcaccg gctggcacag
     4021 tcgcagcgct cgcgcatgct gggcatgctg ctgatcgagt tcaatgccga ctacagcatc
     4081 ccgctgcggc gcgcgcctga cttcagggtg gcatgcgcgc aggcctgggg tgtggctgat
     4141 ccaggcctgg gctgctcggt ggcctcgttg cgcgagttgc tgggcattgg cagcgcccac
     4201 gaatggcggc gcgttggcgt tgaaattgcc gcgctgggtg ctgcacccga taaccgcata
     4261 cacccgcatt acggcgtgtt ttcaccggtg cgtggcgagt acattcagtt gctagccact
     4321 gctcatctgc ccaagccgcc cagcgaccag gccagtctgc tggcgtttga cattggcacc
     4381 ggtaccggcg tgctggctgc ggtgctggcg cgccggggca ttcaacgcat catcgccacc
     4441 gacatcgacc ccagggcgct ggcctgcgcc caggaaaacc tgacctggct cggtcttgac
     4501 aagcaggttg aagtgatcaa ggccgagctg tttccgcctg gccttgcgtc cctgattgtg
     4561 tgtaacccgc cctggctgcc tgcgcggccc ggctcgccac tggagcgcgc ggtgtacgac
     4621 gaaggcgggc gcatgctcaa tggcttcttg cagggcctgc gcgcacacct ggcacccaaa
     4681 ggcgaaggct ggctgatttt gtcgaatctg gccgaacatc tgggcctgcg cacgcgcgag
     4741 gcgctgctgg ccctgtttgc ggacaacggc ttgcgggtca tcagccgcat tgatgcacga
     4801 ccagtacatc ccaaggccag cgacccgacc gatgccctgc accaggcccg cgccaaagag
     4861 gtcacctcgc tgtggcgact ggctgccttg ccagcctagg cgcctgccgg acgaatggat
     4921 cgagccacaa gagtcgcccc cggcaaggcg ggttgctgcc ggatttgtcg gcccatcgcc
     4981 aagtgtggat cagaaggccg gtaaaaaatg gcccgctgcg gccggtgctc agccgttgtg
     5041 ccgcaggccc gtcaggctgc aagagcacct ggccaagcgc aaccgccaga ggatgaagcg
     5101 tcaagccagc gtgcggtgat cgctgccggg ttgattccag gctgacccca attgcccggc
     5161 caaggactgg caaaatggtg aacggataaa ctgaactgca accagatcac tcaaaaccat
     5221 ctcaatgcaa actcgtttat tcccgctgaa actcgcaagc tgcctgttga ccagcagcct
     5281 ggtctggccc ggcgccggtg ccctggccca aagcgctgcg acagtccgcc aggcagcacc
     5341 agcgagcccg gcaccagcca gccccgcatg cccgccaatc ctgaagcaca gctttaaccg
     5401 cctgcaagac gaggccccgc aagacctttg ccagtacgca ggcaaggtca ttctgatggt
     5461 caacaccgcc agttactgcg gattcaccgg gcagtacgaa ggcctggaag cgctgtacgc
     5521 caaatgccag gcccggggtc tggtggtgct gggctttccg tccaacgact ttggccagca
     5581 ggaacccggc agcaacaaag agattgccga cttctgcttt aacacctacg cggtcaagtt
     5641 cccgatgttt gccaaaaccg tggtgtccgg ccccgagcgc aacttgctgt atgccaaact
     5701 ggcgaaagag tccggcacca cacccaaatg gaacttttac aagtacctga tcgaccgcag
     5761 cggccgggtg gtggacagct acaacagcac caccagcccg cagagcaagc gtctggtggc
     5821 ggccatcgaa aagctgttgt gaacgcctga ctctggattc ctcagttgag cacttgtttt
     5881 tatctgcaag gccttatgcg catttgcttc tttagcttcg atggggttca cccgctgggt
     5941 cacagcgcta cgcacccgat tgaaccgctg gcaacgcgcc cagccgcgcc aacagcgccc
     6001 cgcgcgggcg cgtccaactt aatccctaac aggtcaagtc tttgcaaatc atgcgaagaa
     6061 ttcaaacatt caaagtcatt cgtcgcgagg gcgcgactcc tacaaattca aataggcgtc
     6121 gactgtttgt ataggagtcg cgcgctcgcg acgaaggctc atcaatttcg ggtcgtccgg
     6181 cttaggaatc caggctgatg gacaagctgc gcattgacaa gtggctatgg gctgcacgct
     6241 ttttcaagag ccgcagcctc gccagtgatg aaattgacaa aggccgggtg caagtcaacg
     6301 gtgccgcggt gaagcc
//
LOCUS       DAOSLL010000229         6277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11263_length_6277_cov_4.257956, whole genome shotgun sequence.
ACCESSION   DAOSLL010000229 DAOSLL010000000
VERSION     DAOSLL010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6277
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11263_length_6277_cov_4.257956"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..539
                     /locus_tag="PLB25_10940"
     CDS             <1..539
                     /locus_tag="PLB25_10940"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HOE42128.1"
                     /translation="RLLTPVQAQRAGSTYSDLEFDLTRLRSLRERMVELGRSCVHPDY
                     RTGGVIMALWGALAEFMVRNKLDTMIGCASIPMLHNGLASGDVAASIWQQLKTTHLAP
                     IEHRVRPRLPLPVEQFDATLAVEPPALIKGYLRLGAKILGAPAWDPDFNTADLPMLMR
                     INDLPPRYRKHFLGNGAG"
     gene            580..1404
                     /locus_tag="PLB25_10945"
     CDS             580..1404
                     /locus_tag="PLB25_10945"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HOE42129.1"
                     /translation="MGLPAPDADDHEVAPRQRYRAIFISDLHLGTPGCQADALLDFIK
                     ANPSDYLYLVGDIVDGWQLRRRWYWPESHNDVVQKLLRRARKGCRIIYIPGNHDEFAR
                     RYFGHQFGGIEVADDAVHITADGKSLWVTHGDSFDGVIQCAKWLAYLGDNLYEFTLRL
                     NRHLNHWRSRLGLSYWSLSAYLKHKVKKAVMYVTDFEEAVANEAHRRGHDGVVCGHIH
                     RAEMRTINGVLYCNDGDWVESCTALAEHESGLLELVHWVAEPAVRQRSAPVAVVVT"
     gene            1459..3156
                     /locus_tag="PLB25_10950"
     CDS             1459..3156
                     /locus_tag="PLB25_10950"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HOE42130.1"
                     /translation="MFQSSRREFVIRLASVAAVTSAGAVLSACGAGDDPEPPRFDYGV
                     ASGDPLADRVILWTHAQFATTADDVPLTWELAADAAFASIAASGSVKASAATGFTAKV
                     DATGLSAGKDYYFRFRHNGHNSPVGRTRTLPAAGASGLSLAVMTCSNYPAGYFHAYAE
                     IPRTDAQYAVHLGDFIYEYAADGYASADALALGRQSQPANECLTLADYRKRYAQYRSD
                     PDSKTFHASMPVIAVWDDHEVANDSYQSGAENHTEGAEGRFTDRRDAALIAWHEWLPV
                     RTPDATDLRKIYRSFDFGGLAALHMLETRLLGRDKQVSFSDLLNPATAASAMANLTSP
                     TRSLMGSTQVQWLQGQMAASRATWQVLGQQVLMARMNFPVSVLSALNSDNASAEAQAA
                     GQKAISDYLTAKATAAQAPAMLTDTQKALLDPAINPLLGYNLDAWDGYPAAREVVLQT
                     ARQLGKKLVVLAGDTHNAWSSHLTLIDGTVIGQEFATQSFSSPGLEEQLATLTPAQTQ
                     GIFLGVVSDLKYAETARRGFMHMRFTPTAATATWYFVNTVKARAYTVDASATLSYSG"
     gene            4383..4727
                     /locus_tag="PLB25_10955"
     CDS             4383..4727
                     /locus_tag="PLB25_10955"
                     /inference="COORDINATES: protein motif:HMM:NF013868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOE42131.1"
                     /translation="MAIQFEDVTDSFRRIKISGRLDMPGTEAISLKFTNLAASAARRV
                     VVDLTEVSFLASIGIREFITNAKALQLRGGRMILFVGDNASVAKTLQVTGIDALIPMF
                     ADLAQADQAALA"
     gene            4730..5191
                     /locus_tag="PLB25_10960"
     CDS             4730..5191
                     /locus_tag="PLB25_10960"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE42132.1"
                     /translation="MLMTTDTLRQVGDLSIDADGPGTRLASQWLAKLAADFEVPFAEL
                     DRLDICLNEVLANIIRHGTHAVAKPLVHIVFQVERDAGGSLATVTVSDAGPAFDITTA
                     GIHCRPASLTEAEPGGLGLLMIRSFSDELRYHHSDGNNHLSFAVRWNRSDE"
     gene            5184..>6277
                     /locus_tag="PLB25_10965"
     CDS             5184..>6277
                     /locus_tag="PLB25_10965"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HOE42133.1"
                     /translation="MSDGQANRLPSVPTDQFERDVAAIALFRSTDAALILSALRGCEV
                     LHLAAGVPLLRPGEANRHVFIPLSGAIVVHLGSHSSAQATIPIAVGECVGELSAIDGK
                     PVSGLVVALSEARVLKVPQDIFWNRLMTLPGVASNFMVLLSERLRRSTALALKSQSEQ
                     MELMHLRKDLNVARQLQASMLPLQRPLFPERDDIEVCGIMEPASNIGGDLFDAFFVRD
                     RLLFFCIGDVSGHGIASALFMARAIGLLRILAMTTFQPDKLLTKLNQRLCVNNDTNLF
                     LTIFCAFLDMDTGRMVYSNGGHCPPFFGAAGELRDLAIPKGPLVGALPAARFTAMTQT
                     LNRQDVLYCYTDGVTEAQNQAGQEFSEDRC"
BASE COUNT         1213 a         1915 c         1848 g         1301 t
ORIGIN      
        1 atcggctgct gactccggtg caggcgcagc gggccggcag cacctacagc gacctcgaat
       61 ttgacctgac gcgcctgcgc agcctgcgtg agcgcatggt ggaactgggt cgcagttgcg
      121 tgcacccgga ctaccgcacc ggcggtgtga tcatggcgct gtggggggcg ctggcagagt
      181 tcatggtgcg caacaaactc gacaccatga ttggctgtgc cagcattccg atgctgcaca
      241 acggtctggc cagcggcgac gtggctgcca gcatctggca gcagctcaaa accacccacc
      301 tggcacccat tgaacaccgt gtccggccac gcctgccgtt gccggtggag cagtttgacg
      361 ccaccctggc ggttgaaccg ccggcgttga tcaagggcta tctgcgactg ggtgccaaga
      421 tactcggcgc gccagcctgg gacccggact tcaacacggc tgatttgccg atgctgatgc
      481 gcatcaacga tctgccaccg cgctaccgca aacactttct cggaaatggt gctggctgat
      541 gtgatgcggg ctgatttcaa agcgatcggc cccttgatca tgggcctgcc tgcgcctgat
      601 gccgatgatc atgaagttgc gccgcgccag cgctaccggg cgatcttcat ctcggacctg
      661 catttgggca cgccgggctg ccaggccgat gccctgctgg atttcatcaa ggccaaccca
      721 agcgactatc tttatctggt gggtgacatt gtggatggct ggcagttgcg ccgccgctgg
      781 tattggcccg aatcgcacaa cgatgtggtg caaaagctgc tgcgccgggc acgcaagggt
      841 tgccgcatca tctacatccc cggcaaccac gacgaatttg cacgccgtta ctttggccac
      901 cagtttggcg gcattgaagt ggctgacgat gcggtgcaca ttactgctga tggcaaaagc
      961 ctgtgggtga cacatggcga cagctttgat ggcgtgatcc agtgcgccaa gtggctggcc
     1021 tacctgggcg ataacctgta tgagttcacc ctcaggctca atcgccacct gaatcactgg
     1081 cgctcccggc ttggcttgtc ctattggtca ctatccgcct acctcaagca caaggtcaaa
     1141 aaggcggtga tgtacgtgac cgattttgaa gaggcggtag ccaatgaagc ccatcgccgt
     1201 ggtcacgatg gtgtggtatg cggacacatc caccgcgctg aaatgcgcac catcaacggt
     1261 gttctctatt gcaacgacgg tgactgggtg gagagttgca cggcgctggc agagcacgaa
     1321 agcgggctgc ttgaattggt acattgggtc gccgagcccg cagtcagaca gcgcagcgcg
     1381 cccgtggctg tggtggtgac ctgagccgtc agccgcctgc tctgttcgat ttctctcccc
     1441 caacttcaag gaaagaccat gtttcaatca tcccgccgtg aatttgtcat ccgtctggct
     1501 tcggtagccg ccgttaccag tgctggtgcg gtgctgagcg cctgcggcgc tggcgatgac
     1561 cccgagccgc cgcgcttcga ctatggtgtg gccagcggtg acccgctggc cgaccgggtg
     1621 atcctgtgga cccatgcgca atttgccacc actgcagacg atgtgccgct gacctgggag
     1681 ttggctgccg atgccgcatt tgccagcatc gccgccagcg gcagtgtcaa ggcatcagcg
     1741 gccaccggtt tcactgccaa ggtcgatgcc accggattga gcgctggcaa ggactattac
     1801 ttccggtttc gccacaacgg tcacaactcg ccggtcggac gcacccgaac cctgcctgcc
     1861 gccggggcct ccggtctgtc actggcggtg atgacctgct caaactaccc ggcaggctac
     1921 tttcatgcct atgcagaaat ccccaggacc gatgcccaat acgcggtgca cctgggtgac
     1981 ttcatttacg aatacgcagc agacggctac gccagcgccg atgccctggc gctgggtcga
     2041 cagtcgcagc cagccaatga atgcctgacc ctggcagatt accgcaagcg ttacgcgcaa
     2101 taccgatctg accccgacag caagactttt cacgccagca tgccggtaat tgccgtctgg
     2161 gacgaccatg aagtcgccaa cgacagctac cagagcggtg ccgaaaacca caccgagggc
     2221 gcagaaggcc gcttcaccga ccgccgcgat gccgcactga ttgcctggca cgaatggctg
     2281 ccggtgcgca cgccggatgc caccgacctg cgaaaaattt accgcagctt tgactttggt
     2341 ggtctggcag ccttgcacat gcttgaaacc cgtttgctcg gacgcgacaa acaggtcagt
     2401 ttttctgacc tgctcaaccc ggccacggca gccagcgcca tggccaacct gacttcaccc
     2461 acgcgcagcc tgatgggcag cacccaggtg cagtggctgc aggggcaaat ggcggcatcc
     2521 agggccacct ggcaggtgct gggccagcag gtgttgatgg cgcgcatgaa ttttccggtc
     2581 agcgtgctgt ctgcactcaa ctccgacaat gccagtgccg aggcgcaggc ggcgggccaa
     2641 aaagccatct ccgactacct gaccgccaaa gccacagcag cccaggcacc ggcaatgctg
     2701 accgacaccc aaaaggcact gctcgacccg gcgatcaacc cgctgctcgg ctacaacctg
     2761 gatgcctggg acggctatcc cgcagcgcgc gaagtggtat tgcagaccgc ccgtcaactt
     2821 ggcaagaagc tggtggtgct ggctggtgac acgcacaacg cctggtccag ccatctcacg
     2881 ctgatcgacg gcacggtgat cgggcaggag tttgccaccc aaagcttcag ctcgcccgga
     2941 ctcgaagaac aactcgcgac gctgaccccg gcgcagaccc agggcatttt cctgggagtg
     3001 gtcagcgacc tgaaatacgc cgagaccgcg cgccgtggct tcatgcacat gcgcttcacc
     3061 ccgaccgctg caacagccac ctggtacttc gtcaacacgg taaaggcgcg cgcctacacg
     3121 gtcgatgcca gcgcgacgtt gagttacagc ggctagtcga tttatcgctt gagcccgctg
     3181 acggcagcgg cttctttggc caaattgggc cttggccctt cgcccggcaa gccgcgacgt
     3241 gggcggtctg ttcttcacag cccagccccc actctgctgc cttggcgcaa cggcacaaga
     3301 ccaagccaaa ggggatgatc gccccgacac cagcaggcaa actctcaaag cgctgcggtg
     3361 ccagattgcc gtccaagcat ggcttgccag tgttggcgaa tggctgaacc tatcgcgtgg
     3421 ctatctttgg tttgcaatca aaacagaaaa acagcagcgc cgtcgggtag cgcctgaagg
     3481 caccgctgct gcgcagccgg tgattgccgc atttgatgca actcatggtg tggccggagc
     3541 cacccacact ttggtacggt gagccgcttt cctttgaggt gtatcgcaag cctgttggag
     3601 atatctgtgt ggtctcgttt ttgtacatct gggattgctt caatgatttc aatgaaatgg
     3661 actgccagcc cttatggggc gtgcgctggt agctattaaa aacgaagtat ctgagggcgg
     3721 gaatgcaggt ctggatctgg ttctctgctg ctggcctggt tcggtcagac tgggtggtgt
     3781 ggctggccgc caaggcgtgt gaaatgaggt tgggttgcat gaccatgagc ccagaaggtt
     3841 aatcgcgcaa tgtgactcca acatgtcagc gcagttgttg cgttggtccc gacgagatca
     3901 cacgcaacgc gtcgcgacgg gctctgctca cgccaccgcc agccaggacc tttgagccag
     3961 tgccggttgc tcgctcaggc agcaagactg attggcatct tgcaagccaa tcgggcctgc
     4021 ctggaaatgc aggcaccgca ggcgcgccaa tttgcaacga tgggcaatca agcgtaagcc
     4081 gaccacatca gcgtggcacc gcaatgccgc acggtctggc gcacgcaccc gctggctggt
     4141 ttgttcgctt caccagctcc gctgactgct gccccaactt ttctggcaac gccatcgtta
     4201 tgtgaagaat ctggctgcag accttatgaa ataagcgctg gctgctactg ctatcgcagc
     4261 accaggcaac ccggcgttgc aagctgctgg ggcagagaat gtcacaaatt ggtcataaaa
     4321 cctgtgtagc atcgccccgt tgcaattttc ctgcaccgca tttcattcga gaaggtttca
     4381 ccatggccat ccaatttgaa gatgtcacag acagctttcg gcgcatcaaa atctccgggc
     4441 gtcttgacat gccaggcacc gaggccattt cactcaagtt caccaatttg gctgcatcgg
     4501 ctgcgcgccg ggtcgttgtt gacctgaccg aggtcagctt tctggcatcc attggcatcc
     4561 gtgagttcat caccaacgcc aaggcgcttc aattgcgcgg cgggcgcatg attctgttcg
     4621 ttggcgacaa cgcaagcgtg gccaagactt tgcaagtaac tggcattgac gcgctgattc
     4681 ccatgtttgc cgatttggcg caggccgacc aggcagccct ggcctgacca tgctgatgac
     4741 caccgacacc ctgcgccagg tcggtgacct gagcattgac gctgacgggc caggcacccg
     4801 tctcgcgtcg caatggctgg ccaagctggc agccgatttt gaagtgcctt tcgccgaact
     4861 tgaccggttg gatatttgcc tgaacgaggt cttggcgaac atcattcgtc acggcaccca
     4921 tgcggttgcc aagcccctgg ttcatatcgt ctttcaggtt gagcgcgatg ccggtggcag
     4981 tttggccacg gtcaccgttt ctgatgccgg gccggcgttt gacatcacga ccgcaggcat
     5041 tcattgccga ccagcatcgt tgaccgaggc cgagcctggc ggcctgggct tgctgatgat
     5101 ccgcagcttt tcggatgagc ttcgttatca ccacagcgat ggaaacaacc acctgagttt
     5161 tgcggtgcgc tggaacagat cggatgagtg acggacaggc caaccgcttg ccatccgtgc
     5221 caactgatca atttgaacgc gatgttgcgg cgattgcgct gtttcgcagc acagatgccg
     5281 ctttgattct gtcggccttg cggggctgcg aggtgctgca ccttgccgca ggtgtacccc
     5341 tgttgcgacc gggtgaagcc aaccgccatg tctttattcc gctttctggc gcaatcgtgg
     5401 ttcaccttgg cagccactca agcgcgcagg cgacgattcc gattgcggtc ggtgaatgcg
     5461 tcggtgagct ttccgccatc gacggcaagc cggtctctgg cctggtggtg gcactgagcg
     5521 aggcgcgggt gctcaaagtg ccacaggaca tcttctggaa ccgcctgatg acgctgcccg
     5581 gcgtggcatc gaatttcatg gtgttgctga gcgagcgctt gcgtcggtcc accgccctgg
     5641 cgctcaagtc gcaaagcgag caaatggagt tgatgcacct gcgcaaggac ctcaacgttg
     5701 cccgtcaact ccaggccagc atgttgccgc tgcaacgtcc cttgttcccg gagcgcgatg
     5761 acattgaggt gtgcgggatc atggagcctg catccaacat cgggggcgac ctgtttgacg
     5821 cattttttgt gcgtgatcgc ctgttgttct tctgcattgg cgatgtttcc gggcatggca
     5881 ttgcgtcggc ccttttcatg gcccgtgcca ttggcctgct tcgcattctg gccatgacga
     5941 cgttccagcc agacaagttg ctgacaaaac tcaaccagcg tctgtgcgtc aacaacgata
     6001 ccaacctttt tttgaccatt ttttgcgcgt ttctcgacat ggataccgga cgcatggtgt
     6061 attccaacgg gggccattgc ccaccttttt ttggtgccgc cggggaactt cgcgacctgg
     6121 caatcccgaa aggcccgttg gtgggtgccc tgcccgctgc gcgtttcacc gccatgaccc
     6181 aaacgctcaa ccggcaagac gttttgtact gctacaccga tggtgtgacc gaagcgcaga
     6241 accaggcagg gcaggagttt tccgaagacc gctgcaa
//
LOCUS       DAOSLL010000230         6261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11298_length_6261_cov_4.757976, whole genome shotgun sequence.
ACCESSION   DAOSLL010000230 DAOSLL010000000
VERSION     DAOSLL010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11298_length_6261_cov_4.757976"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="PLB25_10970"
     CDS             complement(<1..482)
                     /locus_tag="PLB25_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42134.1"
                     /translation="MAHFAQTYRYAEPSSLNFAGTGQLRLATSSPGAVENPHFFEGSL
                     HQPHLAAQLLSAVHLVVGSRFFTPANSVGLAIALADPVVTAGAGMLRLEGFSACCSTY
                     IRVDLEPQAYAGDVVGKGTTNVDFNAPMRAALARVRDADGLALSVGRSAFALRSGNAQ
                     V"
     gene            complement(1525..1989)
                     /locus_tag="PLB25_10975"
     CDS             complement(1525..1989)
                     /locus_tag="PLB25_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HOE42135.1"
                     /translation="MDESDVEYAGFWIRVGAAIIDTLLIVVVTYPLLVSIYGWKYLDT
                     DKMVAGPADFLISWVFPAIAVIWFWTQKQATPGKAALSLRVLDAGSGENLSIGQSIGR
                     YLAYFVSIIPLGLGLIWVGFDRKKQGWHDKLAHTVVVRAKNHGPEPVRFPQA"
     gene            complement(2016..2507)
                     /gene="arsN2"
                     /locus_tag="PLB25_10980"
     CDS             complement(2016..2507)
                     /gene="arsN2"
                     /locus_tag="PLB25_10980"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HOE42136.1"
                     /translation="MNIEPVSSIEEVSTLLAENALPIADISASSPLQFFGVRDAGALV
                     AVVGLELYRPFGLLRSLAVRADFRRRGLARELVAFAESTSAAQGVASLFLLTTTADQF
                     FLGLGYALASRDQAPSAIQASSQFSSLCPASSAFLSKRVAGRANPVVQETPRDGAAQR
                     PWP"
     gene            complement(2623..3144)
                     /locus_tag="PLB25_10985"
     CDS             complement(2623..3144)
                     /locus_tag="PLB25_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase family protein"
                     /protein_id="HOE42137.1"
                     /translation="MKTIRCTFERHASQILDIFNEAIVTSTALFDYKPRAIESMTAWF
                     KAKESGEFPVIGIEGDNEQLLGFASYGTFRAWPAYKYSVEHSVYVHKAHRGKGLGRKL
                     MVELIAAAQDQNYHLLVAGIEASNSGSIALHESLGFTHSGTLREAGYKFGRWLDLSFF
                     QLTLPTPVQPSEI"
     gene            3486..3704
                     /locus_tag="PLB25_10990"
                     /pseudo
     CDS             3486..3704
                     /locus_tag="PLB25_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011255007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3648..6149)
                     /locus_tag="PLB25_10995"
     CDS             complement(3648..6149)
                     /locus_tag="PLB25_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE42138.1"
                     /translation="MKVQKASILAIDDTPAMLLTLGAALTPEFQLQFATSGSIGLSLA
                     LKEPPSLILLDVMMPGIGGFETCRRLKAEPTLRDIPIVFVTALTDSDSEKEGLALGAA
                     DYITKPVNVGIVRQRIRNLLERERFRKEIEAQRDLLRSALFERQEAEKMVRMLSVAVE
                     QSPASVVVTDLDANVLYVNPRFTECSGYTAAEVIGQNPRIFKSGQTPPHVYQQMWDKV
                     TSGEVWKGTFKNKRKNGELFWEEAHIAGVKDAAGKITHYVAVKLDVSERKQAEDEIRI
                     AAIAFATQSGMVITDASGLILRVNPAFTQLTGYGIEEVAGKSLAMLQADRQAPDFCQR
                     VWETLKETGDWQGAVWNQHKNGEVYAVMLRIKAVVNPDRGTTRYVGAYSDITTDKEAE
                     AEIHRLAYYDPLTSLPNRRLLQDRVSQALAAASRSKHFGALFLIDLDDFKILNDTRGH
                     AVGDELLIEVGRRLRAAVRERDTVARQGGDEFVVLMDAMGNDMALAAAIARQAGEKLR
                     AVTELPFVLSGQEHYCKFSIGIELLRGPELIEDLFKHADLALYQAKRSGRNALCFFDP
                     DMQMALDQRTALIAEMRDAINDGQLRLYYQAQVDSEDQVRGAEALVRWQHPQRGLIPP
                     NEFIPLAEETGLIVPMGLWVLDTACAQIKIWHGTPRFDKLRIAVNVSVNQFRQSDFAE
                     QVLATVARHGIDPCKLKLELTESVMVSDIEDIIVKMNVLKGNGISFSLDDFGTGYSSL
                     VYLKRLPLAQLKIDQGFVRDILDDPNDEAIARLVIALAKSLGLDVIAEGVETQEQRDF
                     LAREGCLSYQGYLFSRPLPLAQFEAFVASSDQTTT"
BASE COUNT         1366 a         1792 c         1788 g         1315 t
ORIGIN      
        1 acttgcgcgt tgcccgagcg cagcgcaaac gccgaacgcc ccaccgacag ggccagccca
       61 tcggcatcgc gcacgcgggc cagggcggcg cgcatgggcg cattgaaatc gacattggtg
      121 gtgcctttgc ccaccacatc accggcatag gcctggggct ccaggtcaac ccggatatag
      181 gtgctgcagc aggccgagaa gccctccagg cgcagcatgc cggccccggc ggtgaccacc
      241 gggtcggcca gcgcaatggc cagcccgacg gagtttgccg gcgtgaaaaa gcgcgaaccc
      301 accaccaggt gcaccgcgct gagcaactgg gccgccaggt gcggttgatg caggctgcct
      361 tcaaagaagt gcgggttttc caccgcccct ggcgacgagg tggccagccg caactgcccg
      421 gtgccggcaa agttcagcga tgagggttcg gcgtagcggt aggtttgagc gaaatgggcc
      481 atgtttgctt tttatcgcgc caaagtcagc ccgctggaac cggtttgcgg ctgaaccatt
      541 ggcgtctggc caacctcccg tttcagtcca gccagggagg tgagggtgat tcgggcgagc
      601 atcgccggat tctgccatgc ctcacctgta aaggcaaaaa agccctctag cccttgtcaa
      661 tcatgcctgg ctagctactt attgcatagc gcttggttgc ccgagccagg ctgccagccg
      721 aaacaccagc accggcaaca gcctttgcag ccaggcgtaa gccgcagtca gccagcacta
      781 tgcggccgca gccagggtca ccgccccggt ggtcgcaaag gcaaatcggc ctgcgtcggc
      841 aaacaggggc atcccacatc tgccggtacg ccagtgggtg cggtctgatc tgacgcgcct
      901 gcgctttctt ctgcagcgtg acgcagccag tgctcgacat ggtgttgcgc atcttgctgc
      961 gggtcattgc gcaaactcct caaaccggct gcactcaaat cgtagccaac ggcggtggcg
     1021 ctaccggatc acctggcaaa tccacagaaa tacaagctaa atcgccctgc agccaccgtg
     1081 gaatatgcgc taccagctat caaataaaga gtgtcttgag cagctcggcc agatcgcatt
     1141 cgctggagat tcttggaatc tggctgtcgc agccaactgc gccgtggcct ccaatgggtt
     1201 catcacctcg accccgtctc gcacggggta gcagttggtc cacgtagagc taaccggcgc
     1261 tgtgcggcta catcgcgcag cgtccggcga ccgaagggag agcgagggtg cagtgggccg
     1321 aaccgcaatt tgtgatcctg aggatccgct ttggcaaggt tggtcttgga agctgacatt
     1381 tccctgtgcc tgtttgcact tataggtctg tgccgtcacg caaacccgga accgcgatga
     1441 gcactgcaag gagattctga agtcgcatga tttgtaccat ctggcaactg ggtgattccg
     1501 gggccgaagg gtggcattga tcggttaggc ttgagggaat ctgacgggtt ctgggccatg
     1561 attttttgcc ctgacaacaa ccgtgtgagc aagtttgtca tgccacccct gctttttacg
     1621 gtcaaaaccg acccatatca ggccgagccc cagcggaatg attgacacga aatatgccaa
     1681 gtagcgtcca atgctttggc caattgagag attctctcct gagccagcat ccaaaacacg
     1741 cagtgacaaa gccgcttttc ctggagtggc ttgcttttgt gtccaaaacc agattaccgc
     1801 gatagccgga aatacccacg aaatcagaaa atctgctgga ccagcgacca ttttgtcggt
     1861 gtcgagatac ttccagccat atattgaaac aagaagcgga taggtgacga ccactatcag
     1921 taatgtgtcg atgattgcgg caccgacacg tatccagaaa cctgcatatt caacatctga
     1981 ttcatccata gatactccta ttgaaagcgt aacgttcaag gccaggggcg ctgcgcggct
     2041 ccatcgcgcg gcgtttcctg caccacaggg tttgcccgcc cggcgacgcg cttggaaaga
     2101 aacgccgacg aagcaggaca aagactggaa aattgggagc ttgcttgaat ggccgaaggc
     2161 gcttggtcgc gagaagcaag cgcgtaccca aggccaagaa agaactgatc ggctgtcgta
     2221 gtgagcagaa acaatgacgc cacgccttgc gcagcagaag ttgactcggc gaacgcaacc
     2281 aactcgcgcg ccagcccgcg cctgcggaaa tcggcacgaa cagcgaggga gcgcagcaag
     2341 ccaaacggcc gatagagttc gaggccaacg actgctacca aagcgccagc atcgcggacg
     2401 ccgaagaatt gcagcggcga cgaggcagaa atatctgcga tcggcaatgc gttctcagcc
     2461 agaagcgtac tgacttcttc gatggaggaa acaggttcaa tgttcatgtc atgtgggcta
     2521 gccgtttgcg ctagccggac cgttgcagca tccggctaaa ggcccagaat ggattggcgg
     2581 acctttggcc gcctgccgca atgggtcggg ttgagcgaac ggttagatct cactgggctg
     2641 aactggtgtt ggtagtgtta gttgaaagaa gcttaaatcg agccagcgcc caaacttgta
     2701 gccagcttct cgtagcgtgc ctgagtgagt aaatccgagc gactcgtgta gagcgatgct
     2761 gcctgagttt gaggcctcta tgccagcgac gagcagatga tagttctggt cctgggcggc
     2821 ggctatgagt tcgaccataa gcttgcgccc aaggcctttg cctcggtggg ctttgtggac
     2881 gtagaccgaa tgctctactg aatacttata agctggccag gctctgaaag tgccgtagct
     2941 agcaaaacct agtagttgtt cgttgtcgcc ttctattccg ataaccggaa attccccact
     3001 ctccttggct ttgaaccaag ccgtcatcga ctctatcgcg cgaggcttgt agtcaaatag
     3061 cgcggttgaa gtgactattg cctcgttgaa gatgtcgagg atctgcgagg catgtcgttc
     3121 aaatgtgcac cgaatggtct tcatggttgg caatgggaaa tagtcttgga aatcgaccta
     3181 acgcatagcg ttgatcttgc gcctgacgac gctgatgaag ccacacaaca cccgccgcgc
     3241 aagataaacc ttgtaggact gaaccgccgt tgaaaggcgg cagcgataag gggattgaaa
     3301 gaaatcctga tactttgtgc gcggctcatg gcccgaactt taccccaccg cgcgcaccag
     3361 actttgatag caggtggcaa gcgccagcag ccagcactga cgtacaagct cacgcttcac
     3421 gcggcggtcc tcgctcgtta gacgtcaaaa catcaccagg gccaggcaag cgttccgagc
     3481 ccgtcatggc gacgctggcg ggccgagcgg cttacgcccg tcgcaaacaa acggtggagc
     3541 cggtgtttgg gatcatcaaa tcggtgctgg ggtttcgcca gttctcactg cgcggtttgc
     3601 gcaaagtcac gggtgaatgg aacctggttt acctgacgcg tcatatttca agtggtggtc
     3661 tggtcagaac ttgcaacaaa ggcctcaaat tgtgcaagcg gtaacgggcg actgaaaaga
     3721 taaccctgat aggaaaggca accttcgcgg gcgagaaagt ccctttgttc ctgggtttcc
     3781 accccctcgg caatgacatc caggcccagg ctcttggcca gcgcgatgac cagtcgtgcg
     3841 atcgcttcat cgtttggatc atcgagaata tcgcgcacga aaccctggtc gattttcagt
     3901 tgcgcaagtg gcaaacgttt caggtaaacc agcgacgaat agccggtgcc aaagtcgtcc
     3961 aaggaaaagc tgatgccgtt gcccttcaaa acattcatct tgacgatgat gtcttcaatg
     4021 tctgagacca tcacgctttc ggtcagttca agcttgagct tgcatggatc aatgccgtgc
     4081 ctggcaactg tggcgagcac ctgctcggcg aagtcgcttt ggcgaaactg gttgacgctg
     4141 acattgacag caatccgaag cttgtcaaag cgtggcgtgc catgccagat tttgatctgc
     4201 gcacaagcgg tgtcgagaac ccagagaccc atcggaacaa tcaatccggt ctcttcggcc
     4261 aagggaataa actcattcgg cggtatcagt ccccgctgtg gatgctgcca ccgcaccagc
     4321 gcctcggcac ccctcacctg gtcttctgaa tccacttgcg cctgatagta gaggcgcaat
     4381 tgcccgtcat tgatggcgtc gcgcatctcg gcgatcaacg cggtgcgctg atccagggcc
     4441 atttgcatgt cgggatcaaa aaagcacagt gcattgcggc cagagcgctt ggcttgatag
     4501 agtgccaggt cggcatgctt gaacaggtct tcgatcaatt ccgggccgcg caacaactcg
     4561 atcccgatgc tgaacttgca gtagtgctcc tgtccgctca ggacgaatgg caattcggtc
     4621 acggcacgca gcttttctcc agcctgtctg gcgatggcgg cagccagtgc catgtcattg
     4681 cccatggcat ccatcaaaac cacgaattca tcgccgccct ggcgggccac ggtgtcgcgc
     4741 tcgcgcactg cggcgcgcaa gcggcgaccg acttcgatca gcaattcgtc accaacagcg
     4801 tggccgcgcg tatcgttgag aatcttgaaa tcatccaggt cgatcagaaa cagtgcgccg
     4861 aagtgcttgc tgcggctggc ggcggcaagc gcctggctga cccggtcttg cagcagccgc
     4921 cggtttggca gggaggtcag cgggtcgtag taagccagcc ggtgaatttc tgcctcggct
     4981 tccttgtcag tggtgatatc tgaatatgcg ccgacgtaac gggtggtgcc ccgatcagga
     5041 ttgacgaccg ccttgattct cagcatcacg gcataaactt caccgttttt gtgctggttc
     5101 cataccgcac cttgccagtc gccggtttcc ttcagcgtct cccagacacg ctggcagaaa
     5161 tcgggggcct gccgatccgc ctgcaacatg gccagcgact tgcctgcgac ttcttcaatg
     5221 ccatagccgg tcaactgggt gaaagccgga ttgacccgca gtatcaagcc gctggcatcc
     5281 gtaatcacca tgccgctttg ggttgcaaag gcgatggcgg caatccgaat ctcgtcttca
     5341 gcctgtttgc gctcggacac atcaagcttg accgcgacgt agtgcgtgat cttgcccgct
     5401 gcgtccttga caccggcgat gtgtgcttct tcccaaaaca attcgccatt cttgcgcttg
     5461 ttcttgaacg tgcctttcca cacctcgccg ctcgtcactt tgtcccacat ctgctgatag
     5521 acatgcggcg gcgtttgccc cgacttgaaa attcgcgggt tctgtccaat gacttctgcg
     5581 gccgtgtagc cggaacactc ggtaaagcgg ggattgacat acagcacgtt ggcatcgaga
     5641 tcggtgacaa cgacagacgc gggtgactgc tcgaccgcga cggacagcat gcgcaccatt
     5701 ttctcggctt cctgtcgctc gaaaagcgcc gatctcagca gatcccgctg cgcctcgatc
     5761 tctttgcgaa agcgctcccg ttcaagcagg ttgcgaatgc gttgtcgcac aatgccgacg
     5821 ttgacaggct tggtgatgta gtcagccgca ccgagcgcca aaccttcttt ttcggaatca
     5881 ctatcggtca acgccgtcac aaagacgatt ggaatgtctc tgagcgtcgg ttcggccttg
     5941 agccggcggc aggtctcgaa gccgccgatg ccgggcatca tcacatccag cagaatcagg
     6001 ctcggcggct ctttcaaggc cagagacaaa cctattgacc cggaggttgc aaactgaagc
     6061 tggaattcgg gtgtcagggc tgctcccaag gtaagcagca tcgcaggcgt atcgtcgata
     6121 gcgagtatgc ttgccttttg aactttcatg cttggtttcc ttgattgggt taaagcgacg
     6181 caaaggaaaa aatcgcgctc agttttaact ggacagtgcc aagttctccc cgttattcag
     6241 gcggtttctt ggccaggcgc a
//
LOCUS       DAOSLL010000231         6250 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11325_length_6250_cov_4.653753, whole genome shotgun sequence.
ACCESSION   DAOSLL010000231 DAOSLL010000000
VERSION     DAOSLL010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6250)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11325_length_6250_cov_4.653753"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..2261
                     /locus_tag="PLB25_11000"
     CDS             18..2261
                     /locus_tag="PLB25_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015041312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE42139.1"
                     /translation="MNCSRPVRRLLLLLIVTALAACTEVSNNPHPAGSEATNTLYVPF
                     SKRSPKNLDPASSYSNDETPYTYQVYEPPYGYHYLKRPYELIGRAAQEVVSPKYLDQA
                     GRVLTDNTPGDQIAETVFDIKIRSGILFAPHPSLAKNGNGDYVYHNMLRSDLDNMYQI
                     SDFKQTGTRELTADDYVYAIRRLATTRVKSPSFSTMADYIVGLKAYGEKIVQMDKKLR
                     AHLAPTNRDLPFLDFRALAFDGATALDRHTLRIRVKGKYPQFKYWLAMTFFAPVPWEA
                     EKFYAQPGMAEKNLTLNYWPVGTGPFMLTEFTENRRHVLQRNPNFRGEPYPCEGEPGD
                     REKGYLADCGKMIPFVDKVVFDIEKEAVPLQAKFMQGYYDSPAIERLDFGTGYIVAMG
                     DDKAKEKEFKEKGIKLPTTVEANNWYLGFNWIDPVVGKGSTPQEAERNRKLRQALAIA
                     IDWEEHIAIFERGQGMAAHGPIPPSLFGYREDGPAAFNPVVYVKGSDGKPVRRPIEDA
                     KALLAQAGYPDGRDAKTGQPLVLNFDYQNSAAGAKALLDWYTKQFAKIGVQMEVRATD
                     YNRFQDKMIKGSVQIFFWGWLADYPDAENFLFMLYGPNSKALTAGNGENGANYQSPEF
                     DALFERMKFLEDGPEKQRLIDQMIEITQKDAVWSFGYFPTSAAAYHQWISNGKPTQIV
                     RNHIGYLRLDPALRAKKIAQWNRPVWWPLPLILLLLLGAVVPAWFAWHRREQETALRE
                     LVGKKEA"
     gene            2258..3235
                     /locus_tag="PLB25_11005"
     CDS             2258..3235
                     /locus_tag="PLB25_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003802581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE42140.1"
                     /translation="MINYILRRIGYGVLILLGVNVLTFILFFAINTPDDMARMNIGGK
                     RVTQDAIDKWKVERGYDKPLLWNDQQTGGQKLTKTIFFERSIPLFVFEFGSSDTGRDI
                     GYELKKRVGPSVALAIPTFLLGLFAVIVFSLILVFFRSTYLEFWGVALAVFLMSISGL
                     FYIIVGQFFFSKLLKLVPISGFAGGWDVFVFLALPVVIGIVSRLGGEALFYRTMFLEE
                     IGRDYVRTARSKGLSETVVLFRHVLRNALLPILTSTVAVLPLLFLGALIMESFFGIPG
                     LGSFTIDAINAQDFAVVRSMVFVGSVLYIVGLILTDISYTIADPRIRLS"
     gene            3250..4743
                     /locus_tag="PLB25_11010"
     CDS             3250..4743
                     /locus_tag="PLB25_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE42141.1"
                     /translation="MPDFVFLMTDLALYGLLAVIALYVRHALHTPTLRQTWRHVLRDP
                     AAMAAGVVLLLFFVLASLDSVHFRPMLPPVPGATPATAPAYSTRTLSVLDALLTGPRE
                     SVEKSYSVPLATHQFSKESVLVNGVSVRDFPRLKFGGGHLKDASAQWAGDVVWRSLAG
                     FAGGALAAAGCWLLVAYGRGARRVEQLRRSAAEIWRGQTDLPWRSILVTASIICLLLG
                     WVTALWPFYHVLGTDQTGNDVLFQAIKSIRTAVVIGSLSTIATLPLAILFGILAGYYK
                     GRVDDAIQYLYTVLSSIPSVLLISAFVLMIQVFIDKNPTLFETGLERADVRLFLLAAI
                     LGVTGWAQLARLLRAETLKLGELDYVQAARAFGVSNSGIMRRHILPNLTHIIVIVSVL
                     DFSGLVLYEAVLSYVGVGVDPSTNSFGSMINGARNEMSRDPVVWWNLLTAFVFMVTLV
                     LSMTIFSSAVREAFDPRARAFRVRGKKAAVFTSSGATEQPQTQDRPC"
     gene            4737..>6250
                     /locus_tag="PLB25_11015"
     CDS             4737..>6250
                     /locus_tag="PLB25_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE42142.1"
                     /translation="MLSVKNLTTEIDSETGLIRAVDALSLTLERGETFALVGESGCGK
                     SMTALSILRLLPDSGRVAGGEVDIDGTEIVLLPESRMRDFRGRRISIVFQEPATSLNP
                     VMTVGRQITEVIERHTRIRGTAAAAKAVDWLQRVGIAEPQRRLHDYPFQLSGGQKQRV
                     MIAIALAAEPDVLIADEPTTALDVTIQAQILDLLRQLQQTQNMAMLLITHDLAIVSQM
                     AHRVAMMYAGQIVEVAEAADFFAQPRHPYAVNLFDALPDASKRGRPLASITGAVPALN
                     QAFVGCRFADRCADVMSRCRTLAPALHEVSPGHSVRCFLYDSSSHQSQPSVGLGRQAA
                     LVADQPTGKTVIELQDYRVWFPIRQGVLQRTVDFVKAVDGVSFTIAAGRTLALVGESG
                     SGKTTVGKALLQLLRHQAQITGSVLLHGQALHLMRGETLRKARRSVQIVFQDPFASLN
                     PRLRLSEILQEGIASLRPEISAVERAARVADLLDKVGVSRAALDRYPHEFSGGQR"
BASE COUNT         1229 a         1669 c         1844 g         1508 t
ORIGIN      
        1 caaccgcaaa ttgtttcttg aactgctcgc ggcctgtccg gcgcctgttg ctgctgctga
       61 tcgtgacggc tttggctgct tgcaccgagg tttccaacaa cccgcatccg gctggctcgg
      121 aagcgaccaa cacgctttac gtgccattta gcaagcgttc ccccaaaaac ctggaccccg
      181 ccagttctta ctccaatgat gaaacgcctt acacctacca ggtgtacgag ccgccctacg
      241 gctaccacta tctgaagcga ccctacgaac tgatcggtcg tgctgcgcaa gaagtcgtgt
      301 caccaaagta tctggaccag gcgggtaggg tgctgactga caacacgcca ggcgatcaaa
      361 ttgccgaaac ggtatttgac atcaaaatca gatctggcat cctgtttgca ccacacccgt
      421 cactggcgaa aaacggcaac ggtgattatg tctatcacaa catgttgcgg tcagatctgg
      481 acaacatgta ccagatcagc gacttcaagc aaaccggtac ccgcgagttg accgcagatg
      541 actatgtcta tgccattcgc cgcctggcaa caaccagagt caagtcgcca tcgttttcaa
      601 ccatggccga ttacatcgtt gggctcaagg cctatggcga aaaaattgtc cagatggata
      661 aaaaactgcg tgctcatctt gcacccacca accgagattt gccgtttctg gatttccgcg
      721 cacttgcctt tgatggcgca actgcgcttg accggcatac gctcaggatc cgcgtcaagg
      781 gcaagtatcc gcagttcaag tattggctgg cgatgacgtt ttttgcaccg gtcccatggg
      841 aggccgaaaa gttttacgca caacccggca tggccgagaa aaacctgact ttgaactatt
      901 ggccggttgg caccggcccc ttcatgttga ccgagttcac cgagaaccgc cgtcatgtgc
      961 tgcagcgtaa ccccaatttc cggggcgagc cctacccctg cgagggcgag cccggcgacc
     1021 gtgaaaaagg ctatctggca gactgcggca agatgattcc atttgtcgat aaggtcgttt
     1081 ttgacatcga gaaagaagcg gtgccgttgc aagccaagtt catgcagggt tattacgact
     1141 caccggcgat tgagcggctg gattttggca ctggctatat cgttgccatg ggtgatgaca
     1201 aggccaagga aaaggagttc aaggaaaaag gcatcaaact cccgaccacg gttgaagcca
     1261 acaactggta tctggggttc aactggattg acccggtggt tggcaaaggc agtacgcctc
     1321 aagaggcaga gcgcaaccgc aaactgcgcc aggccctggc gattgcaatc gactgggaag
     1381 agcacatcgc gattttcgag cgcgggcagg gcatggcggc acatggcccg attccgccat
     1441 cgctgtttgg ctaccgtgaa gatggccctg ccgccttcaa tcccgtggtg tatgtcaaag
     1501 gcagtgacgg caagccggtg cgtcgtccga tcgaagatgc caaagcgctc ctggcacagg
     1561 ctggttatcc ggacggacgt gatgccaaaa ccgggcaacc gctggtgttg aattttgact
     1621 accagaattc cgccgcaggt gccaaagccc tgctcgactg gtacaccaag cagtttgcca
     1681 aaatcggggt tcagatggag gtgcgggcca ccgactacaa ccgttttcag gacaagatga
     1741 tcaaaggctc ggtgcaaata ttcttttggg gctggcttgc cgattatccg gatgccgaga
     1801 attttctgtt catgctgtat ggcccgaact cgaaggcatt gaccgcaggt aacggcgaaa
     1861 atggtgccaa ttaccaaagc cccgagttcg atgccctgtt tgagcgtatg aagtttctgg
     1921 aggatggacc ggaaaagcaa cgcttgattg accagatgat cgaaatcacc caaaaggacg
     1981 cggtctggag ctttggttac tttcctacct cggctgctgc gtatcaccaa tggatcagca
     2041 atggcaaacc gacccagatc gtgcgtaacc acatcggcta cctgcgcctg gacccggcgc
     2101 tgcgggcgaa aaaaattgcc cagtggaaca gacccgtttg gtggccattg ccgctgattt
     2161 tgctgttgtt gctgggtgcg gtggtgccag cctggtttgc ctggcatcgg cgcgagcagg
     2221 aaacagcttt gcgcgagctt gtcggcaaga aagaagcatg atcaactata ttttgcggcg
     2281 catcggttac ggcgtattga tcctgttggg ggtcaatgtg ctgaccttta ttttgttctt
     2341 tgccatcaac accccggacg atatggcaag gatgaacatt gggggcaagc gggttacgca
     2401 agatgccatc gacaaatgga aggtcgagcg cggttacgac aagcctttgt tgtggaacga
     2461 tcaacaaact ggtggccaaa agctgaccaa gaccattttt tttgagcggt cgattcccct
     2521 gttcgtattt gagtttggct catctgacac cggacgcgac attggttatg agctgaaaaa
     2581 gcgggttggt ccttctgtgg cgttggccat cccaactttt ttgcttggcc tgtttgccgt
     2641 gattgtgttt tcgctgattc tggtgttttt cagaagcact tatctggagt tttggggggt
     2701 ggctctggcg gtgttcctga tgtccatctc gggccttttt tacatcattg ttgggcagtt
     2761 ttttttcagc aagctgctca aactggtgcc gatttccggc tttgccgggg gttgggatgt
     2821 gtttgtgttc ttggcactgc cagttgtcat cggcattgtt tcgcgtcttg gtggcgaggc
     2881 acttttttac cgcaccatgt ttctggagga gatcggccgg gactatgtgc gtaccgcgcg
     2941 ctccaaaggc ctgtctgaaa cggtggtgct gtttcgccat gtgctgcgca atgcgctgct
     3001 gccgatcttg accagcacgg tggcggtgct gccgctgctg tttctgggcg cgctgatcat
     3061 ggagtctttt tttggcatac ctggtctggg cagtttcacc attgatgcga tcaatgcgca
     3121 ggactttgcc gtggtgcgct caatggtttt cgtcggttcg gtgctctaca tcgtcggcct
     3181 gatcctcacc gatatctcct acacgattgc cgacccgcgc attcgtctga gttagcttgg
     3241 cttaagaaaa tgcctgattt tgtattcctc atgacagatc tggccctgta cggcctgttg
     3301 gcagtgattg ccctgtatgt caggcatgcc cttcatacgc cgacgctgag acaaacctgg
     3361 cgacacgtct tgcgcgatcc tgcagccatg gccgctggtg tggtgttgct gctttttttt
     3421 gtgcttgcaa gcctggactc ggtacatttt cggcccatgt taccgcccgt gccaggtgcc
     3481 acgcctgcga cagcaccagc ctactcaaca cgaaccctgt cggtgctgga tgccctgctg
     3541 accgggccgc gcgagtcggt tgagaaaagc tactcggtac cacttgcaac acaccagttt
     3601 tcaaaagagt cggtacttgt caacggggta tcggtgcggg attttccgcg tttgaagttt
     3661 ggtggtggcc atctgaagga tgctagtgcg caatgggctg gcgatgtggt ctggcgatct
     3721 ttggcagggt ttgcaggtgg cgctctggca gcggcgggtt gctggctatt ggtcgcatac
     3781 gggcgcggtg cccgcagggt tgagcagttg cggcgcagcg cagcagaaat ctggcggggg
     3841 caaaccgact tgccgtggcg ctcgattctg gtcacggcat cgatcatctg cctgttgctg
     3901 gggtgggtca ctgcactttg gccgttttac catgtgctcg ggaccgatca gaccggcaac
     3961 gatgtgttgt tccaggccat caagtcaatc cgcacagcgg ttgtcattgg gtcgctttca
     4021 accattgcga cgcttccttt ggcaatcctg ttcggcattc tcgcgggata ttacaaaggg
     4081 cgggtggacg atgcgatcca atacctctac accgttttgt cgtccattcc atcggttctg
     4141 ctgatttcgg cgtttgtgtt gatgatccag gttttcattg acaagaaccc gaccctgttc
     4201 gagaccggtc tggagcgagc cgatgtgcgg ctgtttttgc tggctgccat tttgggtgtg
     4261 actggctggg cgcaactggc acgcctgctg cgggcagaaa ccctgaaatt gggcgaactc
     4321 gattatgtgc aggcggcgcg cgcgttcggt gtttccaata gcggcatcat gcgtcggcac
     4381 attttgccca acctgacgca catcatcgtc atcgtgtcgg ttctcgattt ttccggactg
     4441 gtgttgtacg aggcggtact ctcttacgtt ggggtggggg ttgatcccag caccaacagt
     4501 tttggatcga tgatcaatgg tgctcgtaac gaaatgtcgc gcgacccggt tgtctggtgg
     4561 aatcttctga cagcttttgt gttcatggta accctggtgc tgtcgatgac gattttttca
     4621 agcgctgtgc gtgaagcctt cgatccgcgt gcgcgggctt tccgggtgcg tggcaaaaag
     4681 gccgccgtgt tcacaagcag tggcgctacc gagcaaccac aaacccagga tcggccatgt
     4741 tgagcgtcaa aaacctcaca acggaaattg attccgaaac tggactgatc cgtgcggttg
     4801 acgcgttgtc gttgacactg gagcgcggcg agacctttgc actggtcggt gagtctggct
     4861 gtggcaagtc gatgacggcg ctgtcaattc ttcggctgct gcccgacagt ggccgggtcg
     4921 ccggtggcga ggtcgatatc gacggcacgg aaattgtctt gttacctgaa tcgcgcatgc
     4981 gcgattttcg cggtcggcgc atcagcatcg tctttcagga gcccgcaacc agccttaacc
     5041 cggtgatgac ggtcggtcgc cagatcaccg aggtgattga acgacacacc cgcatcagag
     5101 gcaccgcagc ggctgccaag gcggtcgatt ggttgcagcg ggtgggcatt gcagagccgc
     5161 agcggcgctt gcacgactat ccttttcaat tgtcgggcgg acaaaagcag cgcgtcatga
     5221 tcgccatcgc gctggctgcc gaaccagatg tgttgattgc cgatgagcct accaccgcgc
     5281 tggatgtcac gatccaggcc caaatactcg atctgctcag gcagttgcag caaacgcaga
     5341 acatggcgat gttgttgatc acgcatgacc tggcaatcgt gtcgcaaatg gctcatcggg
     5401 tggccatgat gtatgcgggg caaatcgtcg aggtagccga ggcagccgat ttttttgccc
     5461 agccacgcca tccctatgcg gtcaatctat ttgatgcatt gccggatgcc agcaagcgag
     5521 ggcgcccgct tgcctcgatc accggcgcgg tacctgcgct caatcaggcg ttcgtgggtt
     5581 gccgctttgc cgaccgttgt gccgatgtga tgagtcgctg ccgcacgctt gcgccagcgc
     5641 ttcatgaggt atccccgggg cactcggttc gctgcttttt gtatgacagc agcagccatc
     5701 aaagccagcc cagtgttggc ctcgggcggc aagcggcgtt ggtggccgat cagccaactg
     5761 gaaaaacggt gatcgagttg caggattacc gggtctggtt tccgatccgc caaggcgttt
     5821 tgcagcgcac cgttgatttt gtcaaggccg ttgatggtgt ctctttcact attgctgccg
     5881 ggcgcacgct ggccttggtt ggcgaatcag gcagtgggaa aaccactgtt ggcaaggccc
     5941 tgcttcagtt gctgcggcac caggcgcaga tcaccggatc agtgctgctg catgggcagg
     6001 cgcttcatct gatgcgtggg gagaccctgc gcaaggcgcg ccgatcagtg cagattgttt
     6061 ttcaggaccc ttttgcttcg ctcaacccgc gcctgcgcct gagtgagatt ctgcaggaag
     6121 ggatagcctc gctgcggccg gaaatttcag ccgttgagcg tgccgcgcgg gtggcagatt
     6181 tgttggacaa ggtcggcgtg tcgcgggccg cattggatcg ctatccccat gaattctcag
     6241 gtggacaacg
//
LOCUS       DAOSLL010000232         6245 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11334_length_6245_cov_3.865913, whole genome shotgun sequence.
ACCESSION   DAOSLL010000232 DAOSLL010000000
VERSION     DAOSLL010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6245
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11334_length_6245_cov_3.865913"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            4..1164
                     /locus_tag="PLB25_11020"
     CDS             4..1164
                     /locus_tag="PLB25_11020"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008165799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HOE42143.1"
                     /translation="MRRGCVGLAVGCFMAVALAQAASVALKVEVINATLQSPWGLAFL
                     PDGLMLVTEKGGTLKLLAASGQTQGTLGGVPLVNSEGQGGLLDLAIDPAFASNQRIYL
                     SFSESDAANPSLSGTAVARARLDLAANQLRDISVIYRQLPKVNSSGHFGSRLVFDRSG
                     YLFVTLGDRQSRAQRGFAQDLARGNGKVVRITTDGKPAPDNPRFGAAATLPGLWSYGH
                     RNSQGAALHPRTGELWASEHGPQGGDEINLVLPGRNYGWPIISHGQEYGTPLAVGEGT
                     AKEGMEPPVSVWVSRDGSPAPGLFKSSIAPSGLAFYTGDKFPEFQGHLFSGALAGTAL
                     WRVVLGGPQGKREVSRERLLANLGERIRDVRQGADGWIYLLTDSGKLLRLTR"
     gene            1256..1855
                     /locus_tag="PLB25_11025"
     CDS             1256..1855
                     /locus_tag="PLB25_11025"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HOE42144.1"
                     /translation="MKIVLASNNPGKLAELRAMFAPLGFDLLTQGSLGISEAAEPFHT
                     FVENALAKARHAAQHSGLSAVADDAGLCVDAFGGLPGVDTAYYATKFGYAKSDDNNVR
                     ALLEQMQGITNRRAAMVSTLVAVRGAADPEPLVAVGRVVGEIARAPVGSNGFGFDPVM
                     FIPEFGQTFAQLPIEVKNANSHRGRAAKAMVALMRERWL"
     gene            2042..3283
                     /gene="hemW"
                     /locus_tag="PLB25_11030"
     CDS             2042..3283
                     /gene="hemW"
                     /locus_tag="PLB25_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HOE42145.1"
                     /translation="MINVQPDTPQPEVPVPPYDIQQYMRPGTLQLGSLPPLSLYVHLP
                     WCLKKCPYCDFNSHEMRAAELPQQRYIDALMADLEAALPLVWGRAIHSVFIGGGTPSL
                     FSPQAIDDLLAGIRARVPLRPGCEITLEANPGTFEKDRFRAFRAAGITRLSVGVQSFN
                     DDFLKRLGRVHDRAQALAAVQEAAQAFDTFNLDLMYALPGQTLDDLRQDVVTALSLQP
                     PHLSIYHLTIEPNTFFAKYPPPAVDDDTAADMLDLITVMTAQHGLQRYEVSAFARAGQ
                     ACAHNLNYWRFGDYLGIGAGAHGKLSFAHRVLRQVRLRDPARYMAQALAGNAVAQSDE
                     VSRADLPFEFMLNALRLADGFDLTLFTERTGLPMTAIAGALDTAERQRLLQRDLQQVR
                     PSARGFDFLNDLQSLFLPPLR"
     gene            3365..4048
                     /locus_tag="PLB25_11035"
     CDS             3365..4048
                     /locus_tag="PLB25_11035"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOE42146.1"
                     /translation="MFSVYGTSGRLFRGSMEQLRQVGGVAALARSRSVSAVGQDGRDA
                     AGESAAAFSAAKARPASSDDSNRNPLAAYTETITVNPPRHPLSMVRDLMSQTIITLRD
                     TATVLQAWQVLSEKAVGQAPVVDAAGHLVGLLTRADLLKPERLPSPASHALAWSALML
                     QNVTTIMWTPVPSVAPDSDIRRVASVLLDTGLPGLPVVDEAGLVIGFISRSDILRAVV
                     TDPPLDLWG"
     gene            complement(4071..4490)
                     /locus_tag="PLB25_11040"
     CDS             complement(4071..4490)
                     /locus_tag="PLB25_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1850 domain-containing protein"
                     /protein_id="HOE42147.1"
                     /translation="MPLSTLGVCLSLVGALHSTPVFVPVTAFTLAWTHSIEQVRWEED
                     YAVLPGDAPGQPPMLQARQARIKGSAAGMEPPDDAVLQNGWYAYTPTQVPLPELLLTR
                     SEFTADYDWCTLGHCQSMSNLIASDAGVTRARACFYP"
     gene            complement(4516..>6245)
                     /locus_tag="PLB25_11045"
     CDS             complement(4516..>6245)
                     /locus_tag="PLB25_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TRAP transporter permease"
                     /protein_id="HOE42148.1"
                     /translation="LTRMDWVVGVITVALVFEAARRVMGWGLPVICGIFLLYGLFGQH
                     LPGALAHRGFGVDQIVSALGFGTEGIYGTPTYVSSSYIFLFILFGAFLEQAGMINLFN
                     DFAMGMVGHTRGGPAKVAVISSGLMGTINGSGVANVVTTGQFTIPLMKRFGYSSAFAG
                     GVEATASMGGQIMPPVMGAVAFIMAETIDVPYVAIVKAAVIPANLYFITAFWMVHLEA
                     GRKGLLGLAKEHCPNPWTAVRERWYLLLPLIGLVVLLFSGYTPLFSGTVGLALTVVLI
                     FGAAIMSGVQGLALRGLFWVLLGLACAGFFQFGVGTFAVVVAILIAITYARRVGGNTR
                     QLAVQAMVDGARHALPVAIACALVGVIISMINLTGVAAELGGRIISIGESSLFLALFL
                     TMVICLVLGMGIPTIPNYIITSSLAAPVLLKLGVPLLVSHMFVFYFGIMADLTPPVAL
                     AAFAAAPIAHESGLKIGIQAVRIAIAGFVVPYIAVYSPALMLQGGDWLDTSYVVFKAL
                     VAIAMWGAGAIGFLITPLNWFERIVAIVAASFLVAAVPLTDEIGFAAVTAFVAWHVWR
                     SRAGRPSLA"
BASE COUNT         1231 a         1913 c         1920 g         1181 t
ORIGIN      
        1 cgtgtgcgcc gtggctgtgt tggtttggct gtgggttgct tcatggcggt cgcgctggcg
       61 caggccgctt cggtggcgct gaaggttgaa gtgatcaatg cgactttgca aagcccctgg
      121 gggcttgcgt ttttgcccga tggcctgatg ctggtcaccg aaaagggcgg cacgctcaag
      181 ttgctggctg cgtctggcca gacgcaaggc acgctgggcg gcgtaccgtt ggtcaacagc
      241 gaagggcagg gcgggttgct tgacttggca attgatccgg cgtttgccag caaccagcgg
      301 atttacctga gcttttcaga aagtgatgcg gcaaacccca gcttgagcgg cacagcagtt
      361 gcccgtgcac gacttgatct ggcggccaat caactgcgcg atatcagtgt catttaccgt
      421 caactgccca aagtcaactc cagtgggcat tttggttcgc ggctggtttt tgaccgcagc
      481 ggctacttgt ttgtgaccct gggtgaccgc cagtctcgcg cccaacgtgg ctttgcgcaa
      541 gacctggcgc gtggcaacgg caaggtggtg cgcatcacga cagatggcaa accagcgccc
      601 gacaaccccc gttttggtgc ggcggctacg ctgcctggtc tgtggagcta tggtcatcgc
      661 aattcacagg gggctgcgct gcatccgcgc accggcgagc tttgggccag cgaacatggt
      721 ccgcagggcg gcgatgaaat caacctggtc ttgcctggac gcaactacgg ctggcccatc
      781 atcagccatg ggcaggaata cggaaccccc ctcgcggtcg gggagggtac cgccaaggaa
      841 ggcatggaac cacccgtcag cgtttgggtg tcgcgcgacg gcagtcctgc gccgggattg
      901 ttcaagtcgt cgatagcccc cagcggcctg gctttttata ccggtgacaa attcccggag
      961 tttcaaggcc atctgttcag tggtgcgttg gctggtaccg ccttgtggcg ggtcgtgctg
     1021 ggtggcccac aaggcaagag agaagtgtct cgcgagcggc tgctggcaaa cctgggggag
     1081 cgcattcggg acgtccgcca aggggcagac ggctggatct atctgctgac cgattccggc
     1141 aagctattgc gcctgacccg gtgaacggga tcagcaaggc gctgactgtc gtatcattgc
     1201 tattgaagct atagtaagta gcgcatatac atcaagggct ggaggccttt ttcctatgaa
     1261 gatcgtgttg gcgtcgaaca accccggcaa gctggctgaa ctgcgggcca tgtttgcgcc
     1321 cttggggttt gatctgctta cccagggcag cctgggcatt tccgaggcgg ctgagccgtt
     1381 tcacaccttt gtcgagaacg cgctggccaa ggcacgccat gcggcgcaac acagcggttt
     1441 gtcggcggtg gccgatgatg ccggcctgtg cgtggatgct tttggcggtc tacccggtgt
     1501 ggacacggcg tactatgcta cgaaatttgg gtatgccaag agcgacgaca acaatgtgcg
     1561 ggcgctgttg gaacagatgc agggcattac caaccggcgc gccgccatgg tcagcacgct
     1621 ggtggccgtg cgcggtgcag cggacccgga gcccctggtg gcagtcggtc gggtggtggg
     1681 tgaaattgcc cgcgcgccgg tgggcagcaa cggctttggt tttgatccgg tgatgttcat
     1741 ccccgagttt ggccagacct tcgcccagtt gcccatcgag gtcaaaaacg ccaatagcca
     1801 ccggggccgg gcggcaaaag ccatggtggc gctgatgcgt gagcgctggt tgtgatggct
     1861 gttgtcatcg cgatttcggc gtggcgggca tgaccatgga agctgcgggc cggtgtgtct
     1921 caatgcggtt tgctgttgaa cccgcgaggc agaccctctg ggcggcatcc tcatgctgga
     1981 gtaccagaaa tcgtcagccg cccagaggat ctgcctcgct agtcgcttca ggttgccgac
     2041 aatgatcaat gttcagcctg atacaccaca acccgaggtg ccggtaccgc catacgacat
     2101 tcagcaatac atgcgacccg gcacgctgca actgggcagc ttgccaccgc tgtcgctcta
     2161 tgtgcacttg ccctggtgcc tgaaaaaatg cccgtattgc gacttcaatt cgcatgaaat
     2221 gcgcgcggca gagttgccac agcaacgcta catcgatgcc ctgatggccg atctggaagc
     2281 tgccttgccg ctggtttggg ggcgcgccat tcacagtgtt ttcattggcg gcggcacccc
     2341 cagcctgttt tcaccgcagg ccattgacga cttgctggcc ggtatcaggg ccagagtgcc
     2401 gctgcggcct ggctgcgaaa tcacgctgga agccaacccc ggcacctttg aaaaagaccg
     2461 tttcagggcc tttcgggctg ctggcatcac gcgcctgtcg gtcggggtgc agagtttcaa
     2521 cgacgatttt ctaaagcgcc ttggccgggt gcatgaccgt gcccaggcat tggccgcggt
     2581 gcaagaggcg gcgcaagcgt ttgacacctt taacctcgac ctgatgtacg cgctgccggg
     2641 tcagaccctt gatgatctgc gccaggatgt ggtcaccgcc ctgtcgctgc agccgccgca
     2701 cctgtcgatt taccacctga caatcgagcc taacacgttt tttgccaagt atccgccgcc
     2761 tgcggttgat gacgataccg ccgctgacat gctcgacctg atcaccgtca tgacggcgca
     2821 gcacggcttg cagcgttacg aggtttcggc ctttgcgcgc gctggccagg cttgcgcaca
     2881 caacctgaat tactggcgct ttggcgacta tctgggcatt ggtgctggtg cccatggcaa
     2941 gctcagtttc gcccaccgcg tgctgcgcca ggtgcgcctg cgcgatccgg cgcgctacat
     3001 ggcacaggcg cttgctggca atgcggtggc gcaaagcgac gaagtcagcc gcgctgactt
     3061 gccgtttgaa ttcatgctca atgccttgcg cctggctgat gggtttgact tgacgctgtt
     3121 taccgaacgc accgggctgc cgatgaccgc cattgctggc gcgctcgaca ccgccgagcg
     3181 ccagcgcctg ttgcagcgcg acctgcaaca ggtgcgccca agtgcgcgag ggtttgattt
     3241 tttgaacgac ctgcagtcgc tgttcttgcc gccgttgcga tgacaccggc caaaaaaatg
     3301 ctgcgttcgt cggcgcagcg tcttacagta gccatttcaa tgatcgtcac aggagtagcc
     3361 cagcatgttt tcggtttacg gcaccagcgg gcgtttgttt cgcggcagca tggaacaact
     3421 gcgccaggtc ggtggtgtgg ctgcgctggc gcgtagccgc agcgtgtcgg cagtggggca
     3481 agatggtcgc gatgccgctg gcgagtcggc tgctgctttt tctgctgcca aagcgcgccc
     3541 tgccagcagt gacgacagca accgcaaccc gttggcagcc tataccgaaa cgatcaccgt
     3601 caatccgcca cgccacccct tgagcatggt gcgtgacctg atgagtcaga ccatcatcac
     3661 gctgcgcgat accgccacgg tgctgcaggc ctggcaggtg ctcagtgaaa aagcggtcgg
     3721 tcaggcaccg gtggtggacg ctgccggcca tctggtcggt ttgctgacca gggctgacct
     3781 gctcaagcca gagcggctgc cgtcacccgc cagtcatgcg ctggcgtgga gcgccctgat
     3841 gctgcaaaac gtcaccacca tcatgtggac cccggtgccc agcgtggcac cagactctga
     3901 cattcgccgc gtggccagcg tgctgctcga caccggcctg cccggtttgc cagtggtgga
     3961 tgaggccggg ctggtgattg gctttatctc gcgctcggac attttgcgcg cggtggtgac
     4021 cgacccaccg cttgacctgt ggggttaatg accgccgcat caggccggtt ttatggataa
     4081 aaacaggctc tagcccttgt cacacctgcg tcactagcta ttaaatttga catcgactgg
     4141 cagtgcccga gggtgcacca gtcgtaatca gcggtgaact cagagcgtgt caacagcagt
     4201 tcgggcagtg gtacttgggt tggcgtgtag gcataccagc cgttttgcag caccgcatcg
     4261 tcaggtggct ccatgcctgc ggctgatcct ttgatgcggg cttggcgcgc ctgcagcatg
     4321 ggcggctggc cgggtgcgtc gccgggcagc acggcatagt cttcttccca gcgcacttgc
     4381 tcgatcgaat gggtccaggc caaggtaaat gcggtgaccg gcacaaacac cggggtactg
     4441 tgcaatgcgc caaccagact caggcagaca cccagtgttg acagcggcat ggcgaacagg
     4501 gcttgcctgg ctggctcaag ccaggctggg cctgccagcg cggctgcgcc acacatgcca
     4561 ggcgacaaag gcagtgaccg cagcaaaacc gatttcatcg gtcagcggca ccgccgccac
     4621 caaaaaactg gccgccacaa tcgccacaat gcgctcaaac cagttcagcg gcgtgatcag
     4681 aaacccgatg gcaccggccc cccacatggc aatcgccacc aacgccttga acaccacata
     4741 actggtgtca agccagtcgc cgccttgcag catcagggcc gggctgtaga cggcaatgta
     4801 cggcacaaca aaaccggcaa tcgcgatgcg caccgcctgg atgccgatct tcaggccact
     4861 ttcatgcgca attggtgctg cggcaaacgc cgccagcgcc accggtgggg tcaggtcagc
     4921 catgatgcca aagtaaaaca caaacatgtg tgacaccagc agcggcacac ccaacttgag
     4981 caacaccggt gctgccagcg agctggtgat gatgtagttg ggaatggtcg gaatgcccat
     5041 gcccagcacc aggcagatca ccatggtgag aaacagcgcc agaaacaggc tgctttcgcc
     5101 aatgctgatg atgcgcccgc ccagctcggc ggccaccccg gtcaggttga tcatgctgat
     5161 gatcaccccc accagcgcgc aggcaatcgc caccggcagc gcgtggcggg caccatccac
     5221 catggcctgc accgccagtt gacgggtgtt gccgccgacc cgccgcgcat aagtgatggc
     5281 aatcagaatc gccaccacca ccgcaaaggt gcccacgcca aactggaaaa aaccggcgca
     5341 ggccagaccc agcagcaccc agaacaggcc acgcagcgcc agaccctgca cgccgctcat
     5401 gatggccgcg ccaaatatca gcaccaccgt cagcgccagg cccaccgtgc cggaaaacag
     5461 tggggtgtag cccgaaaaca gcaacaccac cagcccgatc agcggcagca gcaggtacca
     5521 gcgctcgcgc acggcagtcc aggggttggg gcagtgttct ttggccagcc ccagcaagcc
     5581 cttgcgaccg gcctccagat gcaccatcca gaaagccgtg atgaagtaca aattggccgg
     5641 aatcaccgct gccttgacga tggccacgta gggcacatcg atggtttcgg ccatgatgaa
     5701 ggccactgca cccatcaccg gcggcatgat ttgaccgccc atgctggcgg ttgcctcgac
     5761 accaccggca aacgccgaac tgtagccaaa gcgcttcatc agcggaatgg tgaactgtcc
     5821 ggtggtcacc acgttggcaa cacccgagcc gttgatggtg cccatcaggc ccgaggagat
     5881 caccgccacc ttggccgggc cgccgcgggt gtgcccgacc attcccatgg caaagtcatt
     5941 gaacaggttg atcatgccgg cctgttccag aaacgcacca aacaaaatga acagaaagat
     6001 gtagctggac gacacataag tgggggtgcc ataaatgcct tcggtgccaa atcccagcgc
     6061 gctgacgatc tggtcaacgc caaaaccccg gtgcgccaat gcaccgggca aatgctggcc
     6121 aaacaggcca tagagtaaaa aaatgccgca aatgacgggc aggccccagc ccatcacacg
     6181 ccgcgcagct tcgaacacca gggccaccgt gatcacaccg accacccagt ccatgcgggt
     6241 caagt
//
LOCUS       DAOSLL010000233         6226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11388_length_6226_cov_4.418733, whole genome shotgun sequence.
ACCESSION   DAOSLL010000233 DAOSLL010000000
VERSION     DAOSLL010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6226
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11388_length_6226_cov_4.418733"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            463..2472
                     /locus_tag="PLB25_11050"
     CDS             463..2472
                     /locus_tag="PLB25_11050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE42149.1"
                     /translation="MNPSDDEEKRSATLLRARAEAKLAGSRVTQTNALPAESLRHELG
                     VHQIELEMQNEELQRSQGELDAVNRRYFDLYDLAPVGYCTLSEAGLILQTNLTASTLL
                     GIERSALVQQPFSRLIVPDDTDGYHLMAKALCKSGEAQTVDLRMMKHDGTHFWAHLVA
                     NASHNADGALVLRLVLSDVSECKQQENKLLESENRLQTILNQSPSLVYVLDLSGRFTL
                     VNKRLAALFGKPNSAILGRTREDFLPTGIAEKHRANDLEIVRTGESTVFEESNDEPNG
                     RRVYLSTKFPLRNMAGAIVAVCGISTDITERKALESEREQYRVDLEERVKSRTQQFLD
                     LYDKAPCGYHSLSHDGVITRVNKTELALLGYSEEEYVGHRIVEFMTLDSVQTFYDAFP
                     LVLQEGRVRNAEFDFVCKDKSIRTFSVNADFVTDSAGAPSYSLSTMVDITERKRYEQR
                     LELALTGADLGLWDVHVPSGEMHLSAKVCSMLGYTEGGIGTHISDLDKLVHPEDANKR
                     RVAIDAANNGDLPDYRVEYRLRHKEGYWEWVRSRGKIVSRDANSFPLRAVGTIKSISQ
                     ERRLRLEGGELLQRIESLIKDFDKATETPPPPTQVAPTNVLGSREQQVIQLIAAGCTT
                     AEIGKHLGISTYTAATHRRNLMRKLNLHTTAELTRFAVTQKLIAD"
     gene            2621..5155
                     /locus_tag="PLB25_11055"
     CDS             2621..5155
                     /locus_tag="PLB25_11055"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE42150.1"
                     /translation="MKNLKIGTRLIILIGTMSALLIGIGIFGMLNLKEASRALEAVYN
                     ENLVPGHALDEINYLMTRNRLVISNTLIDPSPEKISKYTAEVESNVASIGTLWQAFSA
                     SKLSPAMKEAAQQYATHRKEFVEKGLRPAVAALRSNDIKEARRIVLEQIHPLYDATRP
                     YLVKLTEGNLADARNDYELALAEYQNVRNQLIGAVLLGLLLAGGFGWYLVRGITRPLV
                     QAVAAAQAVAEGRFDSDVKATSTDETGQLLAAVHDMQKALLGFEAAQAEMRRQHDLGM
                     IDYVMPADQMQGSYRDLMQSTNDLVASHIAVKMRVVEVVSGYSEGKLEVIMDRLPGQK
                     ARISEAIDKVQQTLQHGAAAATFNLRIRNSLDSLPVNVTVSNADALLVHATPPAKELL
                     KLIAGRGFDADAFYGSKLSSLFTHGESAAQFDRAVKTGAMVDMEIAGRKLRLQARPVL
                     DAAGVPIGRVTQWFDRTDEIAAEEQVAAIVAAAGAGDFSQRLSTAGKSGFLELISVGM
                     NELIATSEQGLGDVANVMAAFAEGDLTQRIKRDYQGLFGKVKDSVNATAENLTRVIGE
                     VHGASDALLGAANQVSATAQSLSQAASEQAASVEETSSQIDVMSASINQNSENARVTD
                     GMATNTSKEAIEGGSAVSHTVAAMKQIASKIGIVDDIAYQTNLLALNAAIEAARAGEQ
                     GKGFAVVAAEVRKLAERSQAAAKEIGELATNSVATSERAGTLLDAIVPSIQKTSELVQ
                     EIAAASVEQSESVVQIGGAMGQLSKATQQNASASEELAATSEELSNQAKLLQESIAFF
                     NVGGDLPKASRRPEGVTNRRIEPVRLVSHVTHAPVRAERGNNFRPY"
     gene            5253..5384
                     /locus_tag="PLB25_11060"
     CDS             5253..5384
                     /locus_tag="PLB25_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42151.1"
                     /translation="MRDTLTRLSLLLNDLKFEDDVQQRQAAHKVAADCIARSRSPQQ"
     gene            5388..>6226
                     /locus_tag="PLB25_11065"
     CDS             5388..>6226
                     /locus_tag="PLB25_11065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOE42152.1"
                     /translation="MTPRVQNCENNKAFAVRLNLPITQLGATFPSGQTLVSVTDTKGR
                     IIYCNPSFVRVSGYSKDELLGQPHNLIRHPDMPAEAFRDLWQTVQAGLPWTGLVKNRS
                     KNGDHYWVRANVTPMREGEQVTGYLSVRTEPSRSEIDAATALYARLQAQAKANKISTG
                     LHHGLVVQTGWMGRLIRSARSALVASGGVEVVVVLVAGFTLASALPFVMQLEWALAVD
                     VALAVMLGRYCRYQARQGLRQITEYATRLAAGDLSGVGTSRQTGAMAQLQLALDQVAV
                     NLRT"
BASE COUNT         1503 a         1672 c         1785 g         1266 t
ORIGIN      
        1 ggaaggtcgg caatatggca gcgatttcga tggtcgccgc acctcctgtt ggccaggtgc
       61 agttgttcga gcatgaccgg tatctggctt ggattcaagg ttgtgaaaaa ctggctcaac
      121 tgtgttgcgg acgaaccaac agcgtggcgc taactttgca gtcgtggtcg cttgggagac
      181 ggtgccaaat ctggtccaac atcagcccat aattccggca gaagaagtgc agtcatctta
      241 atctgtgagc cgaactacca tcattacctt cctatattgc caagtggcga ctgtaagtaa
      301 tttcaccact cgatggcaac gacaagaacg tcctccaccc attgagtagg cgtaacattc
      361 tatgtttggc acatagctgt ataagaggga tttcatggtc cgagaagaac ctatcaagag
      421 ctggatttcc tcgcccaggt gtcgacggca cttagtattg ccatgaaccc gtctgacgac
      481 gaggaaaaac ggtcagcgac tcttttgcgc gcccgagctg aggcaaagtt ggctggcagt
      541 cgcgtgacac agacaaatgc gctccccgcc gagtcgttgc ggcatgaact cggggtgcac
      601 cagatcgaac tggagatgca gaacgaggag ttgcagcgca gtcaggggga actcgacgcc
      661 gtgaacaggc gatatttcga tctgtacgat cttgccccag tgggatattg cacattaagc
      721 gaagctggcc tgatcctgca aaccaacctc accgccagca ccctgctggg cattgaacgc
      781 agcgcgctgg tacaacagcc attctcacgc ttgatcgtcc ctgatgacac ggacggctat
      841 cacctgatgg ccaaagcgct gtgcaagagt ggcgaagcgc agacagtcga cttgcgcatg
      901 atgaagcatg acggcacgca tttctgggcg catctagttg ccaacgcatc gcacaacgct
      961 gacggcgccc tggtgctacg ccttgtactg agcgacgtca gcgagtgtaa gcagcaggag
     1021 aacaagcttc ttgaaagcga gaatagactg caaacgattc tgaatcagtc gccttcgctc
     1081 gtttatgtac ttgacctttc gggtcggttc acactagtca ataaacgact ggctgcgctt
     1141 tttggtaagc caaactccgc aatcctgggt agaacccggg aagattttct tcccacgggc
     1201 attgcggaaa agcaccgcgc caatgacttg gaaattgtgc gaacaggtga atcgacagtt
     1261 tttgaagaga gcaacgatga gccgaatgga cgtcgcgttt acctttccac aaagttccct
     1321 ctgcgcaaca tggccggtgc catcgtggcg gtctgcggga tatccacgga catcaccgag
     1381 cgcaaagcac ttgagtccga acgagaacaa tatcgcgttg accttgagga acgggtaaaa
     1441 agtaggacac agcagttttt ggacctatat gacaaggctc cctgcggata ccactcactc
     1501 tcacatgacg gcgtaattac gcgggtcaac aagaccgaat tggcgcttct cggttactct
     1561 gaggaggagt atgtgggcca tcgaattgtt gagtttatga cccttgatag cgtccaaacc
     1621 ttctacgatg cgtttcctct cgttctgcaa gagggtcgtg taaggaacgc cgagtttgac
     1681 tttgtctgta aggacaaatc cattcgcacc ttttctgtca atgctgattt tgtaacggat
     1741 tcggcaggcg caccctcgta ttccctcagt acaatggtcg atatcaccga acgcaaacgc
     1801 tatgagcaac ggttggagct ggcacttacg ggtgcggacc tcggtctctg ggatgtacat
     1861 gttcccagtg gcgaaatgca cctcagcgcc aaggtctgtt ccatgctcgg ctacacagag
     1921 ggaggcattg gcacacacat cagtgatctc gacaaattgg tgcatcccga agatgcaaac
     1981 aagcggcgag tcgccattga cgctgccaac aatggtgatt tgccggatta ccgggttgaa
     2041 taccgtctgc ggcacaaaga gggctattgg gaatgggtgc gctcgcgtgg caagatcgtt
     2101 tcgcgtgatg ccaactcctt cccgctgcgg gccgtgggaa cgattaaaag tatcagtcaa
     2161 gagaggcgcc tgagattgga aggtggcgag ttgttacaac gtatcgaatc actcatcaaa
     2221 gatttcgaca aagcaacgga gacgccacca ccgcctaccc aagtggcacc aaccaacgtt
     2281 ttgggctcgc gcgagcagca agtgattcaa ttgattgcgg caggatgtac caccgcggaa
     2341 atcggcaagc atctgggcat ttccacctac accgcagcca cccaccggcg caatctgatg
     2401 cgcaaactca atttgcacac caccgcggaa ttgacacgct tcgccgtaac gcagaagctg
     2461 atcgccgact gacatctgca cagatttcca gtaattgata ccttatatta ggtattgagt
     2521 acctaatatg cgtgattgaa aactccattc gattgctcta cattggtgca aagacgcctt
     2581 ggcctgaatg cgtttttcgt tggatcagga cgcagtaggt atgaaaaatc taaaaattgg
     2641 aacccgcctc atcattttga tcggcactat gtcagcgctg ctgattggta ttggcatctt
     2701 tggaatgctg aacctcaaag aagccagtcg ggcactggag gccgtctaca acgagaatct
     2761 ggtcccaggg catgcgctcg atgaaatcaa ttacctgatg acccgcaacc gcttggtcat
     2821 cagtaacacc ttgattgacc cgtcaccaga gaaaatcagc aaatacacgg cagaagttga
     2881 gtccaacgtt gccagcatcg gcaccttgtg gcaggccttc agtgcaagca aactctcgcc
     2941 cgcaatgaag gaggctgcac agcaatacgc cacacaccga aaggaatttg tcgagaaggg
     3001 cttgcgccca gccgtggccg cccttcgctc caacgacatc aaggaggcac ggcgcatcgt
     3061 gctggagcaa attcatccgc tatatgacgc gacccgcccc tatttggtca agctcacgga
     3121 gggcaatctt gccgacgcga ggaatgacta tgaattggca ctggccgaat accaaaacgt
     3181 acgcaatcag ttgatcggcg cggtactgtt gggcctgctg ttggctggtg gctttgggtg
     3241 gtatttggta cgcggcatta cccgcccgtt ggttcaagcc gtagcggcgg cccaggcagt
     3301 tgccgagggg cggtttgaca gcgatgtgaa ggccaccagc acagatgaga ccggacagct
     3361 tttggcagcg gtgcacgaca tgcaaaaggc gctacttggt ttcgaggcgg cgcaggctga
     3421 gatgcgccgc cagcacgatc tgggcatgat cgactacgtc atgccagccg atcagatgca
     3481 gggcagctac cgtgacctga tgcaaagcac caatgatttg gtggcctcac acattgccgt
     3541 caagatgagg gtggtcgagg tggttagtgg ctattcggaa ggaaagcttg aagtgatcat
     3601 ggaccggctt ccaggtcaga aggcgcgcat cagtgaggcg atcgacaagg tgcagcaaac
     3661 cttgcagcac ggcgcagcgg cagccacctt taacctgcgt attcgcaatt ccctagacag
     3721 tctgcccgtc aacgtgacgg tctccaatgc agacgccttg ctagtgcacg ccacgccgcc
     3781 ggccaaggag ctgctcaaac tcatcgctgg ccgcgggttt gatgccgatg ccttttatgg
     3841 gagcaaactc agcagccttt ttacccacgg tgagtcggcg gcccagtttg accgggccgt
     3901 caaaaccggt gcaatggtgg acatggaaat tgccgggcgc aagctgcgtc tgcaggcgcg
     3961 cccggtgctc gatgcagcgg gtgtgcccat tggtcgggtg acacaatggt ttgaccgtac
     4021 cgatgaaatc gcagccgaag aacaagtggc ggccatcgtt gccgcagcgg gcgcgggcga
     4081 cttcagtcaa cgcctgagta ccgcaggtaa aagcggtttt cttgagctta tcagcgtcgg
     4141 catgaacgag ctgattgcca ccagtgaaca aggcctgggt gacgtggcca acgtgatggc
     4201 tgccttcgcc gaaggtgacc tgacccaacg catcaagcgc gactaccagg gcctgtttgg
     4261 caaggtcaag gacagcgtca acgccaccgc agagaacctg acccgcgtga ttggcgaagt
     4321 gcatggtgca tcagacgcgc tgctgggtgc ggccaatcag gttagcgcta cggcgcagtc
     4381 gttgtcccaa gccgccagcg aacaggccgc aagcgtggaa gaaacatcct cgcaaattga
     4441 tgtcatgtcc gccagcatca accagaacag cgaaaacgcc cgggttaccg acggcatggc
     4501 caccaacacc agcaaagagg cgatcgaagg agggagcgcc gtcagtcata cggttgcagc
     4561 gatgaaacaa atagcttcca agattggcat tgtggacgac attgcctacc agaccaacct
     4621 gctggcgctc aatgcagcga ttgaagcggc gcgtgccgga gaacagggaa agggctttgc
     4681 ggtggtcgcc gcggaggtac gcaagctcgc tgagcgtagc caagcggcag ccaaggaaat
     4741 cggggagttg gccaccaaca gcgtggcaac gtcagagcgc gcgggcacgc tgctggatgc
     4801 aattgtgccc agcatccaga aaaccagcga actggtgcaa gagattgcag cagccagtgt
     4861 cgagcaaagc gaatcggtgg tgcaaatcgg cggtgccatg ggacagctca gcaaagccac
     4921 acaacaaaac gcctcggcct ccgaagagct cgcagccacc agcgaagagc tctcaaacca
     4981 ggcgaaactg ttgcaggaaa gtattgcctt cttcaacgtg ggcggggatc tgcccaaagc
     5041 atcccggcga ccggagggcg tcacgaaccg tcgcatcgaa ccagtgcgcc tggtgtcgca
     5101 cgtgacgcat gctcctgtac gcgcggaacg gggcaacaac tttaggccct attgatcccg
     5161 accgcaaagt aattggagcg ccaatggaaa gtccacaaga aagacagcta caggtggaac
     5221 ttgagcgacg actagtggat ggattacacc gcatgcgcga cacactgacc cgcctctcac
     5281 tcttgttgaa tgacttgaaa ttcgaggatg atgtccagca gcggcaggcc gcccacaagg
     5341 tagccgccga ctgcattgct cgcagccgtt cgccccaaca atgatcgatg acgccccggg
     5401 ttcaaaattg cgaaaataac aaggcattcg ccgtgagact aaatttacca attacccagc
     5461 tgggggcaac tttccccagt ggtcaaactc tggtgtctgt tactgacacc aaaggtcgca
     5521 ttatttactg caaccccagc tttgtgagag tcagcggata ttccaaggat gagttgctgg
     5581 gtcaaccaca caatcttatt cgccatcccg acatgcctgc tgaagccttc agggatttgt
     5641 ggcaaaccgt tcaggcgggc ctgccttgga caggactggt aaaaaatcgg agcaaaaacg
     5701 gcgaccatta ttgggtcagg gccaacgtga ccccaatgag ggaaggggaa caggtcaccg
     5761 gctatctctc ggtacgtacc gagccgtcgc gcagcgagat agatgcagcg accgcgcttt
     5821 acgccaggtt gcaggcgcag gctaaggcca acaaaatttc cacggggttg catcacggtc
     5881 tcgtcgtgca aaccggctgg atgggccgcc tgatacgcag cgctaggtcg gctttagttg
     5941 cctcgggtgg tgtagaagtg gtggtggtgc ttgtcgctgg ttttacgctt gcaagtgctc
     6001 taccgttcgt catgcagttg gagtgggcgc tggcagtgga tgtcgctctg gcggtgatgc
     6061 tagggcgata ctgcagatac caggctcgcc aagggttacg acaaatcacc gaatatgcca
     6121 ctcgattggc ggcgggtgat ctgtcaggcg tgggtactag taggcagact ggagccatgg
     6181 cgcagttgca attggctttg gaccaagttg ccgtaaacct tcgcac
//
LOCUS       DAOSLL010000234         6209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11419_length_6209_cov_4.676796, whole genome shotgun sequence.
ACCESSION   DAOSLL010000234 DAOSLL010000000
VERSION     DAOSLL010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6209
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11419_length_6209_cov_4.676796"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2988)
                     /locus_tag="PLB25_11070"
     CDS             complement(<1..2988)
                     /locus_tag="PLB25_11070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE42153.1"
                     /translation="MFGRVVLRWPLVLAAAFCLYTIVLLWNVFGAQTLLRSSADARLI
                     ADSQRRAAAIGDFMVERRNEAVDLSESHEIDAYLVNKALGMSLQYGLIANLDAIDQWF
                     RRKKAQKVLRGTAMFRDIVFFDENGEALSEQSANVGPKPELPAGFEKEAKLVIDPNRR
                     LIVASAPVLHKGIYSGVVVTTGDLTQLAPLLIAGSKSDEVHKYQERLISSDGLNLAAT
                     GNSSSLEKELLQDLLRQPQDVLLSMPELAHALPNAANQLVLRTPVPGLPLSLVNIIDR
                     DTVYGHISSSLFLYSLSVFPFLLLLGAVAFDRQRRRTVQLQHDNSALAEEVARRQALE
                     NELRSNTSRLEQMAVELQHSVQIAQDASRAKSDFLATMSHEIRTPMNGVIGMTDLALE
                     TELTDEQRTYLQIVKSSADGLLTIINDILDFSKMEVGKMAVETIAFDLHSLVATVMKS
                     LSMRAQECSLELICDVHSDVPRQIQGDPGRLRQILINLLNNAIKFTESGEVELGVFVH
                     SDQGEAVQLEFAVRDTGIGIPQDKQKLIFDAFTQEDTSTTRRFGGTGLGLTISSRLAA
                     LMGGRIWVESRSGIGSTFHVQLPFGQTVGGPIPAPMQDLRHKRVLLVDDNAVNRQVLG
                     RMVQRWGMQVTEAASGQSALNILNESAAQGFDLLLLDFHMPGMDGFELTTCLKQDERL
                     RGIKIILLSSVANPGQGVESQEVGIDAYLTKPVLQHELELALRNLFANSAPLPAPPAT
                     NPLTIQDDVKVASGALKILVTEDHPVNQMLVLTLLENWGYHAILAQNGQEALDQHARD
                     VFDLILMDMQMPVMDGLEATRLIRAREASNPDLVKTPIYALTAAVLADEQEAGLRAGV
                     DGYLTKPINKEQFQGVLNQLAASRSVTSRELPGFDYAAALKQCDADILKIIGDKFMAS
                     AASEMAAIEQAIQAANWPELECLVHSQKGLVGNFGANPLEKLFSELEQASRNRRAPDA
                     SLLLELQHEWHRLRKSLGVYLEGK"
     gene            complement(3031..3924)
                     /locus_tag="PLB25_11075"
     CDS             complement(3031..3924)
                     /locus_tag="PLB25_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOE42154.1"
                     /translation="MNMLGKSCLLLASLAMSAALSVAVAADLKSIQAKGELRHLGIRY
                     ANFVTGAGDGFDVELAQGFARHIGVKYTLVYTDFYNVIRDLLGKDVVRKGSEVTLAGD
                     FPVKGDMIATGFTMLPWREAVLLYSKPTFPSQVLLVARADAKFTPIKGSKDLPKDIAE
                     TRSLIGKNSLLVMERTCLDPSNYGLKGVGIDLKAYTKSTNLNEMVPALLNKDAELTLL
                     DVPDAILDLKKWAGQIKVLGPISELQDLAAAFPKDAPELRKAFDEYLVKVKADGSYDK
                     LVDKYYPGIRRYFPDFFARKN"
     gene            complement(4001..4342)
                     /locus_tag="PLB25_11080"
     CDS             complement(4001..4342)
                     /locus_tag="PLB25_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOE42155.1"
                     /translation="MPPDHRQYDAPDEVFERAAGIFRILAAPNRLKIISYLCHGEQNV
                     SYLLDRIDTSQANMSQHLNAMYLARVLKRRRDGVRTYYCMADDRIAHVCKAVCAEVEN
                     RPGFQRLTTAA"
     gene            complement(4456..4881)
                     /locus_tag="PLB25_11085"
     CDS             complement(4456..4881)
                     /locus_tag="PLB25_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HOE42156.1"
                     /translation="MTDCTETRFDMQAAGIAGLPSVLVVCLCARWCDVCTEYLGRFEQ
                     VQFSIQANFPQAQFVWLDIEDEADLLHPLDVENFPTLLIAVGDSPLFFGTITPQAQSL
                     ERLVRHTVLNGAAPEMADPELRAAVTRIRAARQTAVKNQ"
     gene            complement(4874..5983)
                     /locus_tag="PLB25_11090"
     CDS             complement(4874..5983)
                     /locus_tag="PLB25_11090"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HOE42157.1"
                     /translation="MTNPIKRIAVCTGGGDAPGLNAVIQSVVIAADDRGWDCYGIRDG
                     FNGILFPERYAEGGVYRLSRDKVRGIGHLGGTILGTTNKGNPLHFPVNMPDGTVREID
                     RSDEILTYFATHEIDALVSIGGDGSLTIADALHKKGLRVVGVPKTIDNDLDKTYTTFG
                     FDTAVGFATECVDRLHSTAESHQRVMVVEVMGRYAGWIALHVGIASNAHAILIPEIGF
                     DLDKVIAKIRERDANGRLYSIVVVAEGAQARDGHRELLAPAEVGHAERLGGIGESIAH
                     AIASSTGKDTRVVVLGHLLRGGSPTAFDRLAALRFGAAAVRALDAGKSGVMVALGING
                     VNFVALEEVAGRMRNVPLDCDTLQTGRDLGISFGD"
BASE COUNT         1361 a         1812 c         1685 g         1351 t
ORIGIN      
        1 ttttccttca aggtagacac ccagggactt gcgcaaccga tgccattcgt gttgcagttc
       61 caatagcagc gaagcatcgg gtgcccgtcg attgcgggat gcctgctcaa gttccgagaa
      121 caatttttca agcggattgg ccccaaaatt gccgaccagg cctttttgcg aatgcaccag
      181 gcattcgagc tctggccagt tggcagcctg aatagcttgt tctatagcgg ccatctcgct
      241 ggcagccgaa gccatgaatt tgtcgccaat gattttcagg atgtcggcat cacattgctt
      301 caacgccgcc gcgtagtcga aacccggaag ctcccggctt gtcacgctgc gactggcagc
      361 gagttgattc aacacgccct gaaactgctc cttgttgatc ggcttggtca ggtatccgtc
      421 caccccggcc cgcaagccag cttcttgctc gtcggcaaga acggccgccg tgagcgcata
      481 gatcggtgtc ttgaccaggt ccgggttgct ggcttcgcgt gcacgaatca agcgcgttgc
      541 ttccagtcca tccatgaccg gcatctgcat gtccatcagg atcaggtcaa aaacatcgcg
      601 tgcatgctgg tccaatgcct cctggccatt ctgggccagt atggcgtgat atccccaatt
      661 ttccagcaag gtgaggacca gcatttggtt gaccgggtgg tcttcggtga ccagtatttt
      721 cagagcacca ctggccactt ttacgtcgtc ttgaatggtc agggggttgg ttgccggtgg
      781 cgcaggcagt ggggctgaat ttgcaaacag attccgcaag gccagttcca gttcgtgttg
      841 caacactggt ttggtcagat aggcatcaat gcccacctcc tgactttcga caccctggcc
      901 cggattggcg accgaggaaa gcaaaataat cttgatgccc cgcaaccgct catcttgctt
      961 caagcatgtg gtcagctcaa aaccatccat gcccggcata tgaaaatcaa gcaatagcaa
     1021 gtcaaaaccc tgtgcagccg actcattgag gatattgagc gccgactggc ccgatgcggc
     1081 ttcggtaacc tgcatgcccc agcgctgtac catacggccc agaacctgcc gattgaccgc
     1141 gttgtcgtcc accagcaaga cacgtttgtg acgcaggtcc tgcattggcg ctggtatcgg
     1201 cccaccgaca gtctgcccaa acgggagttg aacgtgaaag gtgctgccaa tgccgcttcg
     1261 actctcaacc cagatgcgcc cacccatcaa ggcagccagt cggctggaga tggtcaagcc
     1321 aaggccggtg ccaccaaaac gccgcgtggt ggaggtgtct tcctgagtaa atgcatcaaa
     1381 aatgagtttc tgcttgtctt gtgggatgcc aatgccggta tcacgaacag caaattccaa
     1441 ctggacggct tcgccctgat cgctgtgaac gaataccccc agttcaactt cgccgctttc
     1501 ggtgaacttg atggcattgt tgagcaagtt gatgaggatc tggcgcaagc gccccgggtc
     1561 accctgtatc tggcgcggca catcggaatg cacatcacaa attagctcaa gagagcattc
     1621 ctgagcgcgc atcgacaggg acttcatcac ggtggcaacc agactatgaa ggtcaaaagc
     1681 aattgtttcg acagccatct tgccaacctc catttttgaa aaatcgagaa tgtcgttgat
     1741 gatcgtcagc aagccgtctg cagaagattt gacaatctgg agataagttc gctgctcgtc
     1801 ggtcagctcg gtctccaggg ccaaatcggt catgccgatc accccgttca ttggggtgcg
     1861 gatctcgtgg ctcatggtgg ccagaaaatc agatttggcg cggctcgcgt cttgcgcaat
     1921 ttgcacgctg tgctgcaact cgacggccat ttgctcgagc ctgctggtgt tgcttcgcag
     1981 ctcgttctcc agtgcctggc gccgggcgac ttcttctgcc agcgctgaat tatcatgttg
     2041 caactggaca gttcgccgtc gttggcggtc aaaagcaacc gctcccaaca gcagcaggaa
     2101 cggaaacacg ctcagggaat acaggaacag gctggaactg atgtggccgt aaacggtatc
     2161 ccggtcgatg atgttgacca aggaaagtgg cagacccggg accggagtgc gcagcaccag
     2221 ttgatttgcc gcatttggca gcgcatgtgc cagctctggc atcgacaaca acacgtcttg
     2281 cggctgtcgc agcaggtctt gcagcaattc tttctcaagc gacgatgagt tgccagtggc
     2341 tgccaaattg aggccgtccg atgagatcaa tcgctcttgg tatttgtgca cttcatcgga
     2401 cttgctgcca gcaatcaaca atggggccaa ctgggtcaag tcaccggtag tgacaacaac
     2461 gccactgtag atgcctttat gaagcaccgg agcacttgca acgatcagcc gccgattcgg
     2521 gtcaatgacc agttttgcct ctttctcgaa gcctgcaggc aattcaggtt tggggccgac
     2581 attggcactt tgctctgaca gggcctcacc gttttcatca aaaaaaacga tgtcgcgaaa
     2641 cattgctgtc cctcgcagga ctttttgtgc cttcttgcgt cgaaaccact gatcaatcgc
     2701 gtccaggttg gcaatcaatc catactggag cgacatgcca agggccttgt tgaccaaata
     2761 agcatcgatc tcgtggcttt ctgataaatc gaccgcctca ttgcggcgtt cgaccatgaa
     2821 gtcaccaatc gctgcagccc tgcgctgact gtcggcaatc agccgggcat cggcactgga
     2881 acgcagcagg gtctgggcac cgaagacgtt ccataacaag acaatcgtgt aaaggcaaaa
     2941 cgctgcagcc agcaccaatg gccatcgcaa cacgacacgc ccgaacatcg ctctgtgcct
     3001 tgcttcaagc ctgcgttggc ccgtttgaca tcaatttttt ctggcaaaaa agtcaggaaa
     3061 atagcggcga atgccagggt aatacttgtc aaccagtttg tcgtagctgc catcggcctt
     3121 cactttgacc aaatactcgt caaaggcttt gcgaagttcg ggtgcgtctt tggggaaggc
     3181 cgctgccaaa tcctgaagct ccgaaatggg gcctagcacc ttgatttgac cagcccattt
     3241 tttcaagtcg agaatcgcat cgggcacgtc aagcagggtc aactcggcat ccttgttgag
     3301 caaggccggc accatttcgt tcaagttggt gctcttggta taggccttga ggtcgatgcc
     3361 gactcccttg agtccgtagt tggacggatc cagacaagtg cgctccatca ccagcaggct
     3421 gttcttgcca atcagcgagc gggtctcagc gatgtcctta ggcaaatctt tggagccctt
     3481 gatcggggtg aattttgcat ccgccctggc caccaacagc acttgcgaag gaaacgtggg
     3541 tttggagtaa agcagcaccg cctcgcgcca gggcagcatg gtgaatccgg tggcgatcat
     3601 gtcacccttg accggaaagt caccggccag cgtcacctcg gaccccttac gcaccacgtc
     3661 cttgcccagc agatcacgaa tgacgttata gaagtcggtg tacaccagcg tgtatttcac
     3721 cccgatgtgt ctggcaaaac cctgcgccag ttcgacatca aaaccatcgc ccgcgccggt
     3781 cacaaagttg gcgtatctga tgccaagatg gcgcaactca cccttggcct gaatagactt
     3841 caggtcggcg gcaacggcca ctgaaagggc cgcagacatg gccagactgg ccagcagcag
     3901 gcaagatttt ccgagcatat tcatggtttt atccttttta gcagtgaaac ggcccgtgca
     3961 ggccggaaac atgaggcaac acgcgcttga tccaggtgcg tcaggctgcc gttgtcaatc
     4021 tctggaaccc aggtctgttc tcaacctctg cgcaaaccgc cttgcacaca tgtgcaattc
     4081 ggtcatccgc catgcaatag taagttctga ctccgtcgcg acgccttttc aggacacgcg
     4141 ccaagtacat cgcattcagg tgctgcgaca tgttggcctg tgaggtgtca attctgtcga
     4201 gcaggtagct gacattttgc tcgccgtggc ataagtaact gatgattttc aaacggttgg
     4261 gtgccgccag aatccggaaa atacctgctg cccgctcgaa cacctcatca ggagcgtcat
     4321 attgacgatg gtcaggtggc atggattgaa ttgctgaaac gagaagctca aggctaacag
     4381 tttagctttg cgtgagacct tggccgccta ctgcaaaaag gcataaccga acggctgcca
     4441 tgcttgatcg tcagcttact ggtttttgac cgctgtttgc ctggctgcgc ggattctcgt
     4501 cacggctgca cgaagctctg ggtctgccat ttctggcgcg gccccgttca acacggtatg
     4561 gcgcaccagg cgctcaagag attgcgcctg cggggtgatc gtgccaaaaa acaggggcga
     4621 atcgcctacg gcgatcagca gggtcggaaa gttttcgaca tccaaagggt gcagcaaatc
     4681 ggcttcgtcc tcgatgtcca gccacacaaa ttgcgcctgc gggaaattgg cctgaatgct
     4741 gaactgcacc tgctcgaaac gaccgaggta ctcggtacac acatcgcacc atcgcgcgca
     4801 caagcagacc accagaaccg aaggcaaccc ggcgatgcca gcagcctgca tatcgaaacg
     4861 ggtttcggtg cagtcagtca ccgaacgaaa tgccaaggtc acgcccagtt tgcagggtgt
     4921 cgcagtccag tggcacgttg cgcatgcgcc cggcaacctc ttcgagtgcg acaaagttga
     4981 caccgtttat gcccaacgcc accatcacgc cgctcttgcc ggcatcgagc gcgcgcactg
     5041 ctgccgcgcc aaaacgcaag gcggccaggc ggtcaaaggc ggtcgggctt ccaccgcgca
     5101 gaaggtgacc cagcaccacc acccgggtgt ccttgccggt gctgctggca atggcatggg
     5161 caatgctttc gccgatgccg cccagccgct cggcatgccc tacttcagcc ggtgccagca
     5221 gttcccgatg accatctcgg gcctgcgcgc cttctgccac cacgacgatc gaatacagac
     5281 gaccattggc gtcgcgctca cggatcttgg caatgacctt gtccaggtca aagccgattt
     5341 caggaatcaa gatggcgtgg gcgttgctgg caatgcccac atgcagcgca atccagccag
     5401 catagcgacc catcacctcg accaccatca cgcgctgatg actctcggcc gtgctgtgca
     5461 gacggtccac gcattcggtg gcaaagccca ctgcggtgtc gaagccgaag gtggtgtagg
     5521 tcttgtccag atcgttgtcg atggttttgg gtacaccgac cacccgcagc cccttcttgt
     5581 gcagcgcatc ggcaattgtc agcgagccat caccaccaat tgacaccagc gcatcaattt
     5641 cgtgagtggc aaagtaggtc aatatttcgt ccgagcggtc aatttcccgc accgtcccat
     5701 cgggcatgtt gaccggaaaa tgcagcgggt tgcccttgtt ggtggtgccc aggatggtgc
     5761 cgcccagatg gccgataccg cgcaccttgt cacggctgag tcgatagaca ccgccctcgg
     5821 catagcgctc gggaaacaaa atgccgttga agccgtcgcg tatgccgtag cagtcccaac
     5881 cgcggtcgtc ggccgcgatc acgaccgact gaatcaccgc gttgagaccc ggagcgtcac
     5941 caccaccggt gcagactgca atgcgcttga taggatttgt catgctgatg tcctcggttg
     6001 aatccttgtc taaccaccca ttggcgtaat tgtgccgcaa gccagtcgcc aatcatctcc
     6061 cgaacttcat caaaatgacc acgccagctc gcctggctcg gtcctttggg tccgttttgt
     6121 ggctgaaccg gactggttcg ggtctatttg atcgacagca acgacgggcc agcgtcctgc
     6181 aacgggccaa gcgggttgcg tgtgacttc
//
LOCUS       DAOSLL010000235         6198 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11456_length_6198_cov_4.318411, whole genome shotgun sequence.
ACCESSION   DAOSLL010000235 DAOSLL010000000
VERSION     DAOSLL010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11456_length_6198_cov_4.318411"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            41..1417
                     /locus_tag="PLB25_11095"
     CDS             41..1417
                     /locus_tag="PLB25_11095"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HOE42158.1"
                     /translation="MKRSIDAASRRIATSQILDDLTGLLSRAGFNVALDKAVDQAEAI
                     GGVFCVVYVALDNFGLLNDAFGQVSGDSLLRHVSRRLTVCAGPKAEVCRITSGEFALI
                     VVGDMAYGRSIAARVIEVLAEPVKFGASHTVLSCSIGIAEYPEHGSRAKLLANAALAM
                     RSVKVNGGGDFCQFDPKMGIEVRDVAVLVQDLRSALEMGQLELYFQPKVDAVSLQVTA
                     AEALLRWHHPTRGFVSPVVFIPLAERYGLIGAIGSWVIEEACLKAAGWRERGLRMRVA
                     VNISGYQMREDDLVERVEAALWRNNLRPGRFTCEITESVAMEDTKVTRTTFEKMRQAG
                     FHVSIDDFGTGFSSLATLRRLPAAELKIDRAFVTDLEDSEDARSIARSIVNMAKALNL
                     HVVAEGVETVGQCNLLVEMGCNELQGFLFSKPLRADDLEWLALDSKRQDVAFRPSLFM
                     ETLNATFE"
     gene            complement(1458..1838)
                     /locus_tag="PLB25_11100"
     CDS             complement(1458..1838)
                     /locus_tag="PLB25_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42159.1"
                     /translation="MKYYFIAVCTQSSHGSTRHPQSATLSDSLAVAMAGVGSENFGFV
                     TFSAPPDTSTANSMFGIHRSNGVRSATSTMYQHFLAASHRAPKALTVSFSAKGVTKLI
                     LIAGIIDANTADLPATGGSASGPT"
     gene            complement(1903..2460)
                     /locus_tag="PLB25_11105"
     CDS             complement(1903..2460)
                     /locus_tag="PLB25_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOE42160.1"
                     /translation="MLIGYARVSTQDQNLDLQIDALTKAGCKKLFDDKISGSSKERPG
                     LTKTLEMLREGDTLVVWKLDRLGRSVKNLVDLVSDLHKQGVQFKSLTDAIDTGTPSGR
                     FFFHVMASLAEMERELTVERTRAGLEVARKLGRKGGRKLKMTESKIESAKKLLASGIP
                     PKDVAKNLGVSVPTLYRWIPASSNP"
     gene            complement(2454..2825)
                     /locus_tag="PLB25_11110"
     CDS             complement(2454..2825)
                     /locus_tag="PLB25_11110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003100853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42161.1"
                     /translation="MSTSSIEALANVWARIAEEATLPTGYEGTATPETHRACEAIQAR
                     IRDHIVATNGMRLFGLLHLLGQASLRMEQTLWPEDFERMTREVEEALREADDPNAKLY
                     SHEEAMQAMQERIDRARDKPC"
     gene            complement(2959..3390)
                     /locus_tag="PLB25_11115"
     CDS             complement(2959..3390)
                     /locus_tag="PLB25_11115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HOE42162.1"
                     /translation="MSNQLTPIALMAKADRACLSARALFDLGDVDGACNRAYYAMFDA
                     ARAALLASGAPVRPDIGKTHSGLINAFSEHLIKNGPISKELGRLLKRAEEIRLVADYK
                     GDSVELSDAQEMVAQAETFVTAMHVEFIPNAPSDDDYGMEP"
     gene            complement(3387..3734)
                     /locus_tag="PLB25_11120"
     CDS             complement(3387..3734)
                     /locus_tag="PLB25_11120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOE42163.1"
                     /translation="MGQLNQIDHDTEEAVRRFLALIAGRYDMAGAIVFGSRARGTHRP
                     DSDADVAVLLSGEHQRFLTTKLAMADVAFDVLLETGINISPLPVWLDEWEHPENYSNP
                     ALLQNIASEGFRL"
     gene            3888..>6198
                     /locus_tag="PLB25_11125"
     CDS             3888..>6198
                     /locus_tag="PLB25_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HOE42164.1"
                     /translation="MPRRSILSAAERDSLLVLPDAKDELIQHYTFNETDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGIVLGVGESPLPPLLRLVAAQLKVPVESWDEYGLRDQTRRE
                     HLVELQTVFGFKPFTMSHYRQAVHKLTELALQTDKGIVLASTLVENLRRQTIILPAMN
                     AIERACAEAITRANRRIHAALTDSLLPVHHLRLDELLKRKEGSKTTWLAWLRQSPIKP
                     NSRHMLEHIVRLKAWQALDLPEGIERQVHQNRLLKIAREGGQMTPADLAKFETQRRYA
                     TLVALAIEGMATVIDEIIDLHDRIIGKLFNAAKNKHQQQFQTSGKAINDKVRMYGRIG
                     QALLDAKQSGGDPFAAIETVMPWERFAASVTEAQKLAQPADFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPTAKSVLDAIDVLRTMNTDNARKMPADAPTAFVKPRWAKLVLTGE
                     GIDRHYYELCALSELKNALRSGDVWVQGSRQFKDFDEYLVPVEKFATLKLASELPLAV
                     ATDCDKYLHDRLLLLEQQLATANRLAASNDPPDAIITESGLKVTPLDAAVPDTAQALI
                     DQAAALLPHVKITELLMEVDEWTGFTRHFTHLKTGDTAKDNTLLLTTILADGINLGLT
                     KMAESCPGTTYAKLSWLQAWHIRDETYSTALGELVNTQFRNTFAANWGNGTTSSSDGQ
                     NFRTGSKAESTGHINPKYGSSPGRTFYTHISDQYAPFSTKVVNVGVRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFGLMHLLGF"
BASE COUNT         1355 a         1732 c         1753 g         1358 t
ORIGIN      
        1 gagcaataac gcacgaggcg acaaatggtt gtcatgaatt atgaaaagat caatagatgc
       61 ggcatcaaga cgaattgcaa ctagccaaat cctagacgac ttgaccggcc tccttagtcg
      121 cgccggcttc aatgtagcgc tcgacaaagc agttgatcag gcagaagcga tcggtggtgt
      181 cttttgcgtt gtttatgtgg cgttggacaa ttttggtctc cttaacgatg cctttgggca
      241 agtcagcggc gacagtctgc tgcgccatgt gtcaagacgg ctgacggttt gcgccgggcc
      301 aaaggccgaa gtctgccgta tcacgtctgg tgaatttgcc ctgattgtgg tcggagacat
      361 ggcttacggc cgaagcatcg cggcccgtgt gatcgaggta ttggccgaac cagtcaagtt
      421 tggtgcgagc cacacagtgt tgagttgttc catcggcatt gccgagtatc cggaacatgg
      481 atcgcgcgcc aaactgttgg ccaatgccgc tctggcgatg cgcagcgtca aggtcaacgg
      541 tggcggagat ttttgtcagt ttgaccccaa aatgggtata gaagtgcgtg atgtggcggt
      601 gcttgttcaa gaccttcgca gcgcgcttga gatgggccag ttggagttgt attttcagcc
      661 caaggtggat gccgtcagcc tgcaagtgac cgccgccgaa gcgttgctgc gctggcacca
      721 tcccacacgc ggtttcgtca gcccggtggt tttcattccg ttggcggagc gctacggact
      781 gattggtgcg attggaagct gggtgatcga ggaggcttgc ctgaaagctg ccggttggcg
      841 cgaacgtggc ttgcggatgc gcgtggcggt gaatatttca ggctaccaga tgcgcgaaga
      901 tgacctagtt gagcgcgttg aagctgcatt gtggcgcaac aacttgcggc cgggacgatt
      961 tacctgcgag atcaccgagt cggtggccat ggaagatacc aaagtgacac gaaccacctt
     1021 cgagaaaatg cggcaggcgg gttttcacgt ctccattgat gacttcggca cgggcttttc
     1081 gagtctggcc acgctgcggc gactgcctgc agctgagctg aagattgacc gagcttttgt
     1141 cactgacctg gaagacagtg aagatgcccg ctcaatcgcg cgatccatcg tcaacatggc
     1201 taaggcactc aatttgcatg tcgtggccga gggagtggag accgttggcc aatgcaattt
     1261 gctggttgaa atggggtgca acgagctgca aggattttta ttttcaaagc ccctccgcgc
     1321 cgatgatctc gaatggttgg cgcttgacag caagcgtcag gacgtggcat ttcgcccgtc
     1381 cctgttcatg gagacgttga acgcaacatt tgaatgaatc cgggcagtcc aggcggccaa
     1441 gccagggatc gtgacgctca tgtcgggcca gatgcagagc cgccggtagc aggcaggtct
     1501 gcggtgttgg catcgataat tcccgctatg agaatcaatt tggtcacccc cttcgctgaa
     1561 aaagacacgg tcaaagcctt gggagcacga tgggatgctg ccaaaaagtg ttggtacatc
     1621 gtcgatgtgg ccgatctcac gccgtttgag cggtggatac cgaacatgga gtttgctgta
     1681 gacgtatcgg gtggtgcgct gaaagtgaca aagccaaagt tctcgctgcc aacaccagcc
     1741 attgccacag cgagcgaatc cgataacgtg gcagactgtg ggtgtcgtgt gctgccatgg
     1801 gatgattgcg tgcatacggc aataaagtag tatttcatga cgttggcaaa tgcatgaatt
     1861 gactcaagtg gggtcgtctc agaaaacgga aaataaagca cgttaaggat tactggaggc
     1921 cggaatccag cgataaagcg taggaacaga cacgccgagg ttcttggcca cgtccttggg
     1981 cggaatgccg ctggccagca atttcttggc cgattcgatc ttgctttcag tcatcttgag
     2041 cttgcgacca cccttgcggc caagtttgcg ggcaacttcc agccctgcgc gggttcgctc
     2101 caccgtcagt tcacgctcca tttcggccaa gctggccatg acgtgaaaga agaagcggcc
     2161 tgacggagtg ccggtgtcaa tggcatcggt caggcttttg aactggacac cttgcttgtg
     2221 cagatcgctg accaaatcaa ccaaattttt gacgctacgt cccaaacgat ccagcttcca
     2281 gaccacgagg gtatcgcctt cacgtagcat ttccagcgtc tttgtgagac ctggcctctc
     2341 tttgctactc ccgctgattt tgtcgtcaaa cagcttttta cagccagctt tggttaaagc
     2401 gtcgatctga aggtcaagat tttggtcttg tgtcgagacg cgcgcataac cgatcagcat
     2461 ggcttgtctc gcgcccggtc gatacgttcc tgcatcgcct gcatagcttc ctcatggctg
     2521 tacaacttgg cattgggatc gtcagcctca cgaagtgctt cctcaacctc gcgggtcatg
     2581 cgctcgaagt cttccggcca caacgtttgc tccatgcgca gcgatgcctg gccaagcaga
     2641 tgcagcaggc caaacaaacg catgccattg gtggcgacga tgtggtcacg aatccgcgcc
     2701 tgaatggctt cacaggcccg atgcgtctcc ggtgttgccg ttccctcgta gccggtcggg
     2761 agtgtcgctt cctcggcgat gcgcgcccag acgttagcca gcgcctcgat gctggacgtg
     2821 ctcatttggc ctctgtcaag ccacaacgct ggtgaagctc tgtttgacct gctgcaccgc
     2881 cggcatcacg actgtcagca gctttttttg ctttggctgc cggctttgcg cctgcgccag
     2941 gcttcaaaac gtcattcatc atggctccat tccatagtcg tcatcgctgg gggcattcgg
     3001 aatgaactcg acgtgcatgg cagtaacgaa ggtttcggct tgtgccacca tttcttgtgc
     3061 atcgctcaat tcaactgaat cgcctttgta gtcggccacc agtcggattt cctccgcacg
     3121 cttcaacagc cgccctagtt ctttcgagat aggcccattc ttgatgagat gctcactgaa
     3181 agcattgatt agcccgctgt gcgtcttgcc aatgtcgggt ctaacgggtg cacccgaagc
     3241 cagcaacgca gcgcgggcgg catcgaacat cgcgtaatag gcgcggttgc atgcgccgtc
     3301 cacatcaccc aagtcgaaca aggcccgcgc cgacaaacac gcacggtcag ccttcgccat
     3361 caaggcaatg ggagtcaatt gattgctcac agtcggaatc cctcgctggc gatgttttga
     3421 agtagggccg gattcgagta gttttcgggg tgctcccatt catcgagcca gaccggtagt
     3481 ggtgagatgt tgatgcccgt ttcaagcagc acgtcaaaag ccacatcggc catcgccagc
     3541 ttggtagtca ggaatcgttg atgttctccg ctcaggagca cagcaacatc ggcatcgctg
     3601 tcagggcgat gggtgccacg cgcacgactg ccgaaaacga ttgcgcccgc catgtcatag
     3661 cgaccggcga ttaaggcaag gaaacgccgg acggcttcct ccgtgtcatg gtcaatttga
     3721 ttgagctgcc ccatacatcc atccttatca aaactcgttg taatggtaga ttattgagaa
     3781 taacatatca agaactattt gtgagaatgg aagtgccttg attttgcgtg ccgcatcggt
     3841 gactgggcga ggcttatcaa aaacctgcgt tttcaagagg agcccatatg ccccgtcgtt
     3901 cgattttgtc cgctgccgag cgcgacagcc tgctcgtact acctgatgcc aaagacgagc
     3961 tgatccagca ctacacgttc aacgaaaccg acttgtcgat cattcgccag cggcgtgggc
     4021 ctgcgaaccg tctgggcttc gccgttcagc tttgctactt gcgattccca ggtatcgtcc
     4081 ttggcgttgg tgaatcgcca ctcccaccct tgctgcgatt ggtcgcggcg cagctcaagg
     4141 tgccagtgga aagctgggat gaatacggcc tgcgtgacca gacccggcgt gagcacttgg
     4201 ttgagttgca aacagtgttt ggcttcaagc ctttcaccat gagccactac cgacaagcgg
     4261 tgcataagct gaccgaactg gccttgcaga ctgacaaagg cattgtcctg gccagcacgt
     4321 tggtcgaaaa cctccggcga cagaccatca tcttgcctgc catgaacgcc atcgagcgag
     4381 cctgtgcgga agctatcacc cgcgccaacc ggcgcatcca tgcggcgttg accgattcct
     4441 tgttgcctgt ccatcacctg cgcctggacg aactgctcaa gcgcaaagag ggcagcaaaa
     4501 cgacctggct ggcatggctg cggcaatcgc ccatcaagcc gaattcgcgg cacatgctcg
     4561 aacatattgt gcgactcaaa gcctggcagg cgcttgacct gcccgaaggc attgagcggc
     4621 aggttcacca gaaccgcctg ctcaagattg cccgtgaggg tggtcagatg acacccgccg
     4681 acttggcaaa gttcgagact caacgacgtt atgccactct ggtcgcgctg gctatcgaag
     4741 gtatggccac cgtcatcgac gaaatcattg acctgcacga ccgcatcatc ggcaagctat
     4801 tcaatgcggc caagaataaa catcagcagc agtttcagac ttccggcaag gcaatcaatg
     4861 acaaggtgcg gatgtatggc cgcatcggcc aagccctact ggatgccaaa cagagcgggg
     4921 gcgatccatt cgccgcaatc gaaaccgtca tgccgtggga aaggtttgca gccagcgtca
     4981 ccgaggctca aaagcttgcg caacctgctg atttcgattt tttgcaccgc atcggcgaaa
     5041 gctatgccac gttgcgtcgt tacgcaccag agtttctggc cgtgctcaag ctgcgggcgg
     5101 caccgaccgc caagagcgtg cttgatgcta tcgacgtgct acgcaccatg aacaccgaca
     5161 acgcccgcaa gatgcccgcc gacgcgccca cagcgttcgt caagccgcgc tgggcgaaac
     5221 tggttcttac cggagagggc atcgaccggc attactacga gctgtgcgcc ttgtcggaac
     5281 tgaaaaatgc gttgcgctcg ggcgacgtat gggtgcaagg ttcacgccag ttcaaggact
     5341 tcgacgagta cctcgtgccc gtggaaaaat tcgccaccct gaagctggcc agcgaattac
     5401 cactggctgt ggccaccgac tgcgacaagt acctgcatga ccggctgtta ttgctggaac
     5461 agcagctcgc cacagccaat cgcttggctg cgtccaacga tccgcctgat gccatcatca
     5521 ccgagtcagg actgaaggtt acaccgcttg atgcagcagt gccagacacc gcgcaagcgc
     5581 tgatcgacca agcggcggcg ctattgccgc acgtcaaaat cacagagttg ttgatggagg
     5641 tggacgagtg gacaggcttc acccgacact tcacgcacct gaagaccggc gatacggcca
     5701 aggacaacac cttgctgctg acgacaatcc tagctgacgg tatcaatctt ggcttgacca
     5761 aaatggctga gtcctgtcct ggcacgacct atgctaagct gtcatggctg caagcgtggc
     5821 acatccggga tgaaacgtac tcaacggcat tgggcgagct ggtcaatacc cagtttcgca
     5881 acacctttgc cgcgaactgg ggcaacggca ccacctcatc gtcggacggc cagaacttca
     5941 gaaccggcag taaggccgag agcacaggac acatcaaccc gaagtatgga agcagtccag
     6001 gacggacttt ctacacccat atctctgacc agtacgcacc gttcagcacc aaggtggtca
     6061 atgtcggtgt gcgtgattcg acctatgtgc tcgatggctt gctgtaccac gagtcggatc
     6121 tgcgcatcga ggaacactac accgacacgg cgggcttcac cgatcacgtc ttcggcctaa
     6181 tgcacttgct gggtttcc
//
LOCUS       DAOSLL010000236         6197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11458_length_6197_cov_4.774503, whole genome shotgun sequence.
ACCESSION   DAOSLL010000236 DAOSLL010000000
VERSION     DAOSLL010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6197
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11458_length_6197_cov_4.774503"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1998
                     /locus_tag="PLB25_11130"
     CDS             <1..1998
                     /locus_tag="PLB25_11130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOE42165.1"
                     /translation="AGFDEPGQPASFKPLRPADIAILVRTGKEAAAVRRELQRRSVDS
                     VYLSDKDSVFDSREAHDLVHWLRAVATPQDSRLVRAGLATRTLGLSLAELGWLAANDE
                     AFDARSEQLRQLRSVWQMQGVLAMLRQTLHQLGLAARWLAAPNGERKLTNFLHLAELL
                     QTASSTQDGEQSLIRWLVTEVNEGSAQGDEQVVRLESDADLVKVITIHKSKGLEYPLV
                     CLPFASSFRAKDSKTTSFVNLANEDGQRELRLQFSKDELALADRDRLREDLRLVYVAL
                     TRARHALWVGFSAVKSGTGSDCVSHHSGTGYLIGGTEPIAAADWLATLEQFAATCAGM
                     QLLAAEPDTACTLLSHSVADAPLLERPNYQADFERHWTIGSFSGLARGLTPTGATSAL
                     SPLQAPRPADDEPDLMPDAAAPPEPTGAAANPAAWHQFARGAEAGNFLHEQLEWLASE
                     RFALADNAPMATRLQRRCERAGRGAQAQDVLLWLTDVVQTRLPGPDAALVDLVNLMPE
                     MEFWLPANCMQAKEVDAQCRLHLLPGLDRPALVERQLHGMLMGFADLVFEHQGKYWVL
                     DYKSNHLGEDDAAYHPDALAHAMTEHRYDVQAAIYLLALHRLLKQRLGSNYLPEEHLG
                     GAVYLFLRGIKGAQNGVCLIPACQPLLNALDAMLAQESLPA"
     gene            2220..2447
                     /gene="vapB"
                     /locus_tag="PLB25_11135"
                     /pseudo
     CDS             2220..2447
                     /gene="vapB"
                     /locus_tag="PLB25_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589461.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
     gene            2616..2909
                     /locus_tag="PLB25_11140"
     CDS             2616..2909
                     /locus_tag="PLB25_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HOE42166.1"
                     /translation="MRWITFFSDTPDMMEIRKERQNLHFEYLKKHESEILIAGGCREV
                     PGGAFVGGLWVLEVPSKERAVELIENDPYFVPSCRTYQLLVWGKAFAEKPVVL"
     gene            3007..3414
                     /locus_tag="PLB25_11145"
     CDS             3007..3414
                     /locus_tag="PLB25_11145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006050037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOE42167.1"
                     /translation="MRYLLDTNFCVYLLDAHPPQVVQRFKTAALGQVGMSVVTYAELC
                     HGVDRFVGAERDVAAKSLARLTLRVPVVAFESSAAESYGVVAAAVRDRRRDTMDQLIA
                     AHAVSAGLTLVTNNEADFKDYPGLMVENWVSAP"
     gene            3549..5624
                     /gene="recD"
                     /locus_tag="PLB25_11150"
     CDS             3549..5624
                     /gene="recD"
                     /locus_tag="PLB25_11150"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HOE42168.1"
                     /translation="MIDSALIVEPDSTPELLADPLVILHSWADLGWLRRLDSALASFM
                     RELDPDASPVLLVSSAVLAQMEGRGHTCLPLRRLVAAPDEVLAWPAVAQDALNALWQT
                     LPASLADWLAALRASSLVRTATLSPCADATQDQPDRGQPLVLGGTETEPLLYLRRYWL
                     HERQVAAEVLHRTGASQQVDEGLARSWLDSLFDPAASDAPVANQVDWQKLACAVALRA
                     SLTVITGGPGTGKTYTAARLLALLFAMDKEPQRLRVALAAPTGKAAARLRQSIDASLQ
                     ELNMRLGDQLDLATLTKRIGAARTLHALLGARPDTRQLRFNASHLLDVDILIVDEASM
                     IHLEMMSALLQAMPAGARLILLGDKDQLASVEAGAVLGDLCRDAALGDYDQETARYAL
                     VVAGQTLAPEFLACGSHTMALAQQTVMLRQSRRFGGLIGQLALAVNGGNGERAQSLIT
                     EDQTAVLQATYHPSVETVLNLAVHGRPGASACFADYLRLISERPAQAWQDVSSHAAWV
                     KGVLVAFDRFRLLCAVHDGDWGTRSLNQAVQQALAGAGLLKAQGEWFAGRPVMVTRND
                     PELGVFNGDVGVALPGIRGSTALRVFFLDGDSLRSVGVSRLAQVETAFAMTVHKSQGS
                     EFLHTALVLPPGGAKVLTRELVYTGITRARERFTLLEGQAGLLQKAIARPSQRASGLG
                     LQLEHSAGI"
     gene            complement(5941..6150)
                     /locus_tag="PLB25_11155"
                     /pseudo
     CDS             complement(5941..6150)
                     /locus_tag="PLB25_11155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018575094.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1190 a         1866 c         1906 g         1235 t
ORIGIN      
        1 gccggttttg acgagcccgg tcagccagcc agcttcaagc cgctgcgccc cgctgatatc
       61 gcgattctgg tgcgcaccgg caaggaagcg gcagcggtgc ggcgcgaact gcagcgtcgc
      121 tcagttgatt cggtctacct gtccgacaag gattcggtgt ttgacagtcg cgaggcgcat
      181 gacctggtgc actggttgcg cgctgttgcc acgccacagg actcccggct ggtgcgcgcc
      241 gggctggcca cccgcacgct tggcctgagt ctggctgaat tgggctggct ggctgccaac
      301 gacgaagcgt ttgatgcccg cagtgaacaa ttgcgccaat tgcgcagcgt ctggcaaatg
      361 caaggcgtgt tggccatgct gcgccagacc ttgcaccaac tgggactggc agcgcgctgg
      421 ctggcggccc cgaatggcga gcgcaaactg accaatttcc tgcacctggc agagttgctg
      481 caaaccgcga gcagcaccca ggacggcgag cagtcgctga ttcgctggct ggtcaccgag
      541 gtgaacgagg gcagcgccca gggcgatgaa caggtggtgc ggcttgaaag tgatgccgat
      601 ctggtcaagg tcatcaccat tcacaaaagc aaagggctgg aataccccct ggtttgcctg
      661 ccttttgcca gcagtttcag agcaaaagac agcaaaacca cgtccttcgt caacctcgcc
      721 aatgaggacg gccagcgtga attgcggctg cagttcagca aggacgaact ggcgctggct
      781 gaccgtgacc gcctgcgcga agacttgcgg ctggtgtatg tggcgctcac gcgcgcccgc
      841 catgctctgt gggtggggtt ttctgccgtc aaatcaggga ctggttcgga ctgcgtctct
      901 caccacagtg gcacaggtta tctgatcggc ggcaccgaac ccattgccgc cgccgactgg
      961 ctggcgacgc tggagcagtt tgccgccacc tgtgcgggca tgcagttgct ggcagcagag
     1021 cctgataccg cctgcaccct gttgagccac agcgtggcag atgccccgct gctggagcgg
     1081 cccaactacc aggccgactt cgagcggcat tggacgattg gcagtttttc tggcctggcg
     1141 cggggcttga ccccgaccgg cgcaacgtct gccttgtcgc cactgcaagc gccccgaccg
     1201 gcagacgatg aaccggacct gatgcccgat gccgctgccc caccagaacc caccggcgcg
     1261 gcagccaacc cggcagcctg gcaccagttt gcacgcggcg ccgaggctgg caactttctg
     1321 cacgagcaac tcgaatggct ggcaagcgag cgttttgcct tggctgacaa cgcgccaatg
     1381 gccacgcgcc tgcaacgccg ctgtgagcgc gccggccgtg gcgcgcaggc gcaagatgtg
     1441 ctgctgtggc tgactgatgt ggtgcaaacc aggctgcccg gacccgacgc ggcactggtc
     1501 gatctggtca acctcatgcc cgaaatggag ttctggctgc cagccaattg catgcaggcc
     1561 aaagaagtcg atgcccagtg ccgcttgcac ctgttgccgg ggcttgatcg gccagcgctg
     1621 gttgagcgcc agttgcacgg catgctgatg ggttttgccg acttggtgtt tgagcaccaa
     1681 ggcaaatact gggtgctgga ctacaagtcc aatcatctgg gcgaagacga tgcagcctat
     1741 caccctgacg ctctggccca cgccatgacc gagcaccgct atgacgtaca ggccgcgatt
     1801 tacctgttgg cgctacaccg cttgttgaaa cagcgcctgg gaagcaacta tctgcccgaa
     1861 gaacacctgg gcggtgcggt ttatctgttt ctgcgcggca tcaagggtgc gcaaaacggg
     1921 gtttgcctga tccccgcctg ccagcccctg ctcaatgcgc tggatgccat gctggcgcaa
     1981 gaaagcctgc cagcatgagc cgccgtttca atgccgcccg ccataccgca gttcaactgc
     2041 caaaacacag cgtagcccgc atcagcattg ccaggaaagc tatcgataga gaagcaaaaa
     2101 tgaatgcccg gccaagcgtt gctgagccat tgctgaccga ccaattgaag agcggtccgg
     2161 tagcgctttg aatacttgta tagcctgtat atacttcgat ttttcaagga gaaaacttca
     2221 tgttgacccg ggttttcaaa agcggcaatt cgctggccgt gcgcatcccc aaagagctgg
     2281 cctttgccga tggcgcgcag gacgtagaaa tcgagcgtgt cggcaacacg ctggtgctgc
     2341 gcccggtggt gcaggaaacc attggcgacc tgaccgagat ttttgctgcc ttcccaaaaa
     2401 gcttcatggc cgagggccgt gagtttcatg aacaacgtga gcgcgactgg gggtgtgttg
     2461 gcttggacag agccaataac gcacccaatg ccaccccaag cgcgcccggc gagagggtgc
     2521 cggcttgact ttcatggact ctgcaccccc ccccggacat gaaagctatt tcacgttaga
     2581 acctttccaa ccccagactt acttacggag atttcatgcg ctggatcacc ttcttttcag
     2641 acacacctga catgatggaa attcgaaagg agcgacaaaa tttgcatttc gaatatctca
     2701 aaaaacatga gtctgaaatc ctcatcgcgg gtggctgccg agaggttcca ggcggtgctt
     2761 ttgtcggagg gctctgggtt ttagaggttc cctccaaaga gcgtgctgtt gagctgatcg
     2821 aaaatgaccc atatttcgtc ccgagttgcc gcacctacca gttgcttgta tggggcaagg
     2881 cgtttgccga gaagcctgtc gtcctgtgat acgccgaggc tctaacatgg cgctcaacct
     2941 cgctccctgc ggtccctgga cgaccccaag ctccgctttg tctcgccggt tagctctaca
     3001 ttaatcatgc gctacctgct cgacaccaat ttctgcgttt acctgctcga tgcccatcca
     3061 ccgcaagtgg tgcagcgctt caagaccgct gctttggggc aagttggcat gtcggtggtg
     3121 acctacgctg agctttgcca cggtgttgac cgctttgttg gtgccgaacg tgatgtggca
     3181 gccaaatcgc ttgcgcgcct gacactgcga gtgccggtcg tggcttttga gtcaagcgcc
     3241 gccgaaagct acggcgtggt ggccgcagcg gtgcgtgacc ggcgccgcga caccatggac
     3301 cagctcatcg ccgcccatgc agtgagcgca gggctgacgc tggtcaccaa caacgaggcc
     3361 gatttcaaag actaccccgg tctgatggtt gaaaactggg tcagcgcccc atgaacagca
     3421 ccagaacggc caatcaatct tttaagccaa atagccctct agcccttgtc atataagcgt
     3481 aagcagctat caaaacaata acaaaaaaca tcccttcact gctgacccat gaccaaagcc
     3541 acaccatcat gattgattcc gccttgatcg tcgagcccga cagcacacct gaactgctgg
     3601 ccgatccgtt ggtcattctg cacagttggg ccgacctggg ctggctgcgc cgcctcgaca
     3661 gcgcgctggc cagcttcatg cgcgagcttg accctgacgc atcgccggtg ttgctggtaa
     3721 gtagcgccgt gctggcgcaa atggaggggc gcgggcacac ctgcctgcca ctgcggcggc
     3781 tggtggcagc gcccgatgag gtactggcct ggccagcagt cgcccaggac gcgctgaacg
     3841 ccctttggca aaccctgcca gccagtctgg ccgactggct ggccgccctg cgggccagtt
     3901 ccctggtgcg cacggccacc ctgtcgccct gcgcggatgc aacacaagac caacctgatc
     3961 ggggtcaacc gctggttctg ggcggcaccg agaccgagcc cttgctgtat ttgcggcgtt
     4021 actggctgca tgagcgccag gtggctgccg aggttctgca ccgcaccggg gcaagccaac
     4081 aagtggatga aggcctggcg cgcagctggc tcgacagctt gttcgaccca gcggccagcg
     4141 atgcgccggt tgccaaccag gtggactggc aaaagctggc ctgtgcagtg gccttgcgcg
     4201 ccagcctcac cgtcatcacc ggtggccctg gcactggcaa aacctacacg gcggcccgtt
     4261 tgctggccct gctgtttgcc atggacaagg agccccagcg tctgcgcgtc gcgctggcgg
     4321 caccaactgg caaggctgcg gccagactgc gccagtcgat tgatgcgtcc ttgcaggagc
     4381 taaacatgcg cctgggcgac cagcttgatc tggctacatt gaccaaacgc attggcgcag
     4441 ccagaaccct gcacgcgctg ttgggcgctc gccctgacac ccggcagttg cgcttcaatg
     4501 ccagccatct gcttgatgtg gacatcctga tcgttgacga ggcctccatg atccacctgg
     4561 agatgatgtc ggccctgctg caagccatgc cagcgggtgc ccggctgatt ctgctgggcg
     4621 acaaggacca gttggcctcg gtggaggccg gtgccgtgct gggcgacttg tgtcgcgatg
     4681 ccgcgctggg tgactacgac caggagacgg cccgttatgc gctggtggtg gccgggcaaa
     4741 cgctggcgcc tgagtttctc gcctgcggct ctcacaccat ggcattggcg cagcaaaccg
     4801 tcatgctgcg ccaaagtcgc cgctttggcg gattgatcgg acaactggcg ctggcggtca
     4861 atggcgggaa cggcgagcgg gcccagtcgc tcatcacaga agaccagaca gccgtgctgc
     4921 aggccaccta tcacccgagt gtcgaaacgg ttttgaactt ggccgtccac ggtcgccctg
     4981 gtgccagcgc ctgctttgcg gactacctgc gactgatcag cgaacgtccg gcacaggctt
     5041 ggcaagatgt cagcagtcat gccgcctggg tcaagggcgt gctggtggct tttgatcgct
     5101 ttcgtctgct gtgcgcggtc catgacgggg attggggcac ccgcagcttg aaccaggcgg
     5161 tgcagcaagc gctggcaggc gctggcctgt tgaaggcaca gggtgaatgg tttgcgggtc
     5221 gtccggtgat ggtcacccgc aatgacccgg aacttggggt tttcaatggc gatgtcggcg
     5281 tagcgctgcc cggcatccgg ggcagcaccg cactgcgggt gtttttcctg gacggcgaca
     5341 gcttgcgctc ggttggtgtg agtcgcctgg cccaggtcga aacggctttt gccatgacgg
     5401 ttcacaaatc gcagggctcc gagttcttgc ataccgcgtt ggtgctgccg ccaggcggtg
     5461 ccaaggtctt gacgcgtgaa ctggtttaca ccggcatcac ccgcgcgcgc gagcgcttta
     5521 ccctgcttga ggggcaggcg ggtttgctgc aaaaggcgat agcgcggccg tcacaacggg
     5581 ccagtggact gggcctgcag cttgagcatt cagccggaat ttgacatcgc tgacagcgct
     5641 gtgtcgtgcc gccgcttgcg cacccaacgc agacaatcaa aactcccgcc ccaaggggcg
     5701 gggtaaaagt cccactacgt ctcgcacgct tcgcggctcg acttcgtttg tacagtgccc
     5761 tacggacact gtattttcca aaaaaccggg gacaagtacc gccccaaggg gcggggcgtt
     5821 tacccatagt gaattaaggg gcacgcgttg gcacggcacg cttgagtgat tgggttcggg
     5881 aaatttttgt ggcattactg tgttggattg tttgcaagaa agcaggtaag cggatgccca
     5941 tcacgtattg acaccagtag tgccgagatg gcaaagcgtg gcgcagttac ggttttgaga
     6001 ctgccagcga tggggcggca attgttcaat ctgactgttt ttctgcgtag gcagacgagt
     6061 cagcacatcc ttgatatagg catacgggtc atgcccgttg agtttggcgc tctgaatcaa
     6121 actcatcaca gtagcgacgc gctgcggtgc agaggggttt gttcgatgag cgcaatttgc
     6181 tggagatcag ctcagcg
//
LOCUS       DAOSLL010000237         6195 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11459_length_6195_cov_4.426547, whole genome shotgun sequence.
ACCESSION   DAOSLL010000237 DAOSLL010000000
VERSION     DAOSLL010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6195
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11459_length_6195_cov_4.426547"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="PLB25_11160"
     CDS             complement(<1..190)
                     /locus_tag="PLB25_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42169.1"
                     /translation="MLITQAPDEHSDHERLQLASEVLRNGTSQQLVAQIDLKVAGFAF
                     LVHPVCCASRLRPTKSEHC"
     gene            complement(677..997)
                     /locus_tag="PLB25_11165"
     CDS             complement(677..997)
                     /locus_tag="PLB25_11165"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HOE42170.1"
                     /translation="MNGDLVLALAGMVVVTYGIRLSFLVFGHRLSFPPWLESALRYVP
                     AAVLTALIVPMALAPQGSIDLSLGNAYLPGTVVAAGIAFITRHTLGAILGGFVVYGLW
                     RWLI"
     gene            complement(994..1650)
                     /locus_tag="PLB25_11170"
     CDS             complement(994..1650)
                     /locus_tag="PLB25_11170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HOE42171.1"
                     /translation="MLVGAAPFGVIFGTLAIAGGIPVWATLALSAVVFAGSSQFIAVS
                     LISGGAALPVLWLTTFVVNLRHALYSATLLPYARHLPARWRWPLAFWLTDETFAVVEN
                     QLRHHVGERNGAWYWLGSSLAMYLNWQVWTVTGVLVGQSVPGLATLGLDFAMVATFAA
                     IVAPQLRDRPVLLAALAAGGIALLARDLPYKLGLMLAASLGVIAGVVAERRIRQGRAG
                     "
     gene            complement(1690..3150)
                     /gene="tldD"
                     /locus_tag="PLB25_11175"
     CDS             complement(1690..3150)
                     /gene="tldD"
                     /locus_tag="PLB25_11175"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HOE42172.1"
                     /translation="MISREPTLERLGVAKSLLLTPFGLDESHLARALAEIKAHKVDEA
                     DLYFQYTRSEGWSLEEGIVKTGSFSIDQGVGVRAVSGEKTAFAYSDDISAASLLDAAR
                     TVRSISTVAQTGRARVASKKIASSRSLYPGLDPIASLDSSTKVGLLMKVEQLARSKDP
                     RVAQVMAGLAAEYDVVMVARADGTLAADVRPLVRLSVTVIAEQKGRREVGSAGGGGRF
                     GFAYFDDALIGQYVDEAVNAALTNLDARPAPAGEMTVVLGPGWPGILLHEAIGHGLEG
                     DFNRKGSSAFSGRVGQRVAAKGVTVLDDGTISDRRGSLNVDDEGNPSGRNVLIEDGIL
                     KGYIQDAMNARLMGVAPTGNGRRESYAHVPIPRMTNTYMLGGDKSSEEIVASIKKGLY
                     ATNFGGGQVDITSGKFVFSASQAYWVENGKILYPVKGATLVGSGPECLKRVSMIGNDM
                     KLDSGVGTCGKEGQSVPVGVGQPTLRIDGLTVGGTA"
     gene            complement(3268..4422)
                     /gene="rodA"
                     /locus_tag="PLB25_11180"
     CDS             complement(3268..4422)
                     /gene="rodA"
                     /locus_tag="PLB25_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HOE42173.1"
                     /translation="MSLVFERPSAWQRLSPWFTGFDGPLAFAVFLLACAGLMAMYSSG
                     YDHGSRFEDHGRNMLIAGFIMFTVAQIPPQRLMALAVPVYVLGVALLIAVAVFGITKK
                     GARRWLNVGVIIQPSEILKIAMPLMLAWWFQKREGQLRPLDFLVAGVLLAVPVGLIIK
                     QPDLGTALLVLAAGLSVIFFAGLSWKLVAPPLLIGLIGITLLVLFEPELCADGVNWPG
                     LRAYQQQRICTLLDPTRDPLGKGFHIIQGMIAIGSGGFWGKGFMQGTQTHLEFIPERT
                     TDFIFAAFSEEFGLFGNLLLIVAFIFLVLRALAIALDAPTVFSRLLAAAVAMIFFCYA
                     FVNMGMVSGILPVVGVPLPFISYGGTAMVTLGLGLGILMSIAKSKRLVQS"
     gene            complement(4432..4899)
                     /gene="dtd"
                     /locus_tag="PLB25_11185"
     CDS             complement(4432..4899)
                     /gene="dtd"
                     /locus_tag="PLB25_11185"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HOE42174.1"
                     /translation="MMALLQRAALARVDIAGQTVGRIGAGLLVLLCAERGDTVAQSDK
                     LLEKILKLRIFNDAAGKMNHSLQDIDGLGRVGELLVVSQFTLAADTSGGNRPGFSGAA
                     LPADGLHLYNHFVAQARLRHPVVQTGEFGADMQVHLINDGPVTIPLRVTSGCR"
     gene            complement(5191..>6195)
                     /gene="tyrS"
                     /locus_tag="PLB25_11190"
     CDS             complement(5191..>6195)
                     /gene="tyrS"
                     /locus_tag="PLB25_11190"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOE42175.1"
                     /translation="HTVIFLIGDFTTLIGDPSGRNSTRPPLSREQIEVNAQTYYRQAS
                     MVLDPNKTEIRYNSEWGDALGARGMIGLAARYTVARMMERNDFHDRFQSGTPISVHEF
                     LYPLMQGYDSVALKSDLELGGTDQKFNLLVGRHLQAEYGQEPQCILTMPLLEGLDGIE
                     KMSKSKNNYIGISEDANTMFAKCLSISDVLMWRWYTLLSFQSEAQIAALKAEVDGGRN
                     PKEAKVALAKEITTRFHCAAAADAAEQDFINRSKGGIPAEIADIRLALGADGAALGIG
                     ALLKSAGLAASSGEGNRLIDGGGVRVDSGVVSDKGLKLGAGTYVLQVGKRKFARVTLA
                     "
BASE COUNT         1378 a         1971 c         1733 g         1113 t
ORIGIN      
        1 agcagtgttc ggatttggtc ggtcgtaaac gggacgcaca gcaaaccggg tggacgagaa
       61 acgcaaaccc ggctaccttc aggtcgattt gggcaacaag ctgctgcgaa gtgccgttgc
      121 gcaacacttc tgatgccaac tgcaaccttt catggtcgct gtgctcatca ggcgcttgag
      181 ttatgagcat cgctttcttt tgcttcgatg aggttcaccg attgggtgac agcgctactc
      241 acccgattga accgctggca acgcgactga gggtgtcgac ccgaggagtc tgggtttgtt
      301 gcggttgccg cttgtgcccc tgacttgacg aaaatcatga acacctgtcg ccctcatgat
      361 ccgccccctt ttgagtaact gatggcaggg tgaaaaggca ggggaaactt gtcgctgtcc
      421 agtaaccctg caatctcaga tgacacgcaa acggcgaagc tgttttgacg gtgtttttta
      481 agcactaatt ggcccacatc ccttgtcaga caagcgtagt gagctatcaa ttttgaaagt
      541 tttgagtagc agggaatttc aggcaatggc atgtcctcat cacagccaat ggaaatgacc
      601 agttgccacg tagggctgtc agccgccttt ttgcgcccgg cggcacagca cagcccgggg
      661 attggttgga gccgggtcaa atcaaccagc gccaaagccc ataaaccaca aaccccccca
      721 agatcgctcc cagagtgtgc cgggtaatga atgcaatgcc tgctgccacc acggtccccg
      781 gcaagtacgc attgcccagc gacaggtcga tgctgccttg cggtgccagc gccatcggca
      841 cgatgagtgc ggtaagcact gccgctggca catagcgcag cgcactttcg agccagggcg
      901 gaaagctcaa gcggtgtccg aacaccagaa agctcaggcg aatgccatag gtcaccacta
      961 ccatgccagc aagggcaagc acaagatcac cgttcacccc gccctcccct gtctgattcg
     1021 gcgctcggcc accaccccgg cgatgacacc caggctagct gccagcatca gacccagttt
     1081 gtagggcaga tcgcgtgcca gcaaagcgat gccgcccgcc gccagcgcag ccagcagcac
     1141 cgggcgatcc cgcaattgcg gtgccacgat ggctgcaaag gtggcaacca tcgcaaaatc
     1201 aagccccaag gtggccaaac ctggcacgct ttgcccgacc agcacgcctg tgacggtcca
     1261 gacctgccaa ttgaggtaca tggccagtga tgaacccagc cagtaccagg caccattgcg
     1321 ctcgcccaca tggtggcgca actggttttc caccacagca aaggtttcat cagtgagcca
     1381 gaacgccaac ggccagcgcc agcgggcggg caagtgccgg gcgtagggca gcagcgtagc
     1441 gctgtacaag gcatggcgca gattgaccac aaaggtggtc agccacagca ccggcaaagc
     1501 ggcaccgcca ctgatcaggc tgaccgcaat gaactggctg gaaccggcaa acaccacggc
     1561 agacagcgcc agcgtggccc acaccggaat accaccagca atcgccaatg tgccaaaaat
     1621 caccccaaaa ggcgctgcgc ccaccagcat tggcacggtg tcacgcgcgc cgcaccaaaa
     1681 tggactcaat taggccgtac ccccaacggt gagcccgtca atgcgcagtg tcggctggcc
     1741 gacccctacc ggcacactct ggccttcctt gccgcaggtg ccaacgccac tgtcgagttt
     1801 catgtcgttg ccaatcatgc tgacgcgctt caggcattcg ggaccactgc cgaccagcgt
     1861 ggcacccttg accgggtaca aaatcttgcc gttttccacc cagtaggcct ggctggcgga
     1921 aaacacaaat ttgccggatg tgatatcaac ctggccaccg ccaaaattgg tggcgtaaag
     1981 gccttttttg atgctggcga cgatttcttc agacgacttg tcgccaccca gcatgtaggt
     2041 gttagtcatg cgcggaatcg gcacatgggc atagctctcg cgtcggccat tgccagtcgg
     2101 tgccaccccc atcaggcggg cgttcatcgc gtcctggatg tagcccttca gaatgccgtc
     2161 ttcaatcagc acattgcgcc cactcgggtt gccctcgtca tcgacattga gcgagccgcg
     2221 ccggtctgaa atggtgccgt cgtcgagcac cgtgacaccc ttggccgcca cccgctgtcc
     2281 gacacggcca ctgaaggcgc tggacccctt gcggttgaag tcaccctcga gcccgtggcc
     2341 aatggcttcg tgcagcaaga tgccgggcca ccctgggccc aggaccaccg tcatttcacc
     2401 agcgggggcg ggccgtgcgt ccagattggt cagcgcagcg ttcaccgcct catcgacata
     2461 ctggccaatc agcgcgtcgt caaaataggc aaaaccaaag cggccaccgc caccggccga
     2521 gccgacttca cggcggcctt tttgctcggc aatcaccgtc acgctcaggc gcaccagcgg
     2581 gcgcacatcc gccgccagcg tgccgtcggc gcgtgccacc atcaccacat cgtattcggc
     2641 cgccaggccc gccatcacct gtgcgacgcg cgggtctttg ctgcgcgcca gttgttccac
     2701 cttcatcagc aggccgacct tggtgctgct gtcaaggctg gcaatcgggt caagaccagg
     2761 gtaaagagac cgcgaagatg ctattttttt agaagctacc cgcgcacgtc ctgtctgggc
     2821 tacagtcgaa attgaccgta cagttcgggc ggcatcaagc aacgaggctg cggaaatatc
     2881 gtcagagtag gcaaaggcgg ttttctcacc actgacggcg cgcacaccga caccctggtc
     2941 aatcgagaac gaaccggtct tgacaatgcc ttcttccaga ctccagccct cgctgcgggt
     3001 gtactgaaaa tacaggtcgg cctcgtccac cttgtgcgcc ttgatctcgg ccagcgcacg
     3061 cgccaggtga gattcgtcca gaccaaaagg ggtaagcaac agggacttgg caacgcctaa
     3121 gcgttcgagg gtgggttcgc gtgaaatcat gcgcccgatt ctaggagcct ggggcattga
     3181 tggatcgaac agcgggaatc gaccccagcg aggccaggct gtgtcggcta tttgactggt
     3241 cagggccaag tcaccgacca cgaagtctca ggattgcacc agccgcttgg acttggcaat
     3301 cgacatcagg atgcccagcc cgagcccgag cgtgaccatg gcagttccgc cgtagctgat
     3361 aaagggcagc ggcacgccca ccaccggcaa aatgccgctg accatgccca tgttcacaaa
     3421 ggcataacaa aagaaaatca tggcaacggc tgctgccaac aaacgcgaaa aaaccgtggg
     3481 ggcatcgagc gcaatggcga gggcacgcaa caccagaaaa atgaaggcaa caatcagcag
     3541 caggttgcca aaaaggccaa attcttcaga aaacgcggca aaaataaaat cggtggtgcg
     3601 ctcggggatg aattccagat gggtttgtgt gccctgcatg aagcccttgc cccagaagcc
     3661 gccggaacca atggcgatca tgccctggat gatgtgaaag cctttgccaa gcgggtcgcg
     3721 ggtcgggtcc agcagcgtgc agatacgctg ctgctgatag gcccgcaggc cgggccagtt
     3781 cacgccatcg gcgcacaact cgggctcaaa cagcaccagc agggtgatgc caatcagccc
     3841 gatcaacagc gggggagcca ccagcttcca gctcagcccg gcaaaaaaaa tgaccgatag
     3901 gccagccgcc agcaccagca gcgctgtgcc cagatcgggc tgcttgatga tcagcccaac
     3961 gggcactgcc agcagcacgc cagcgaccaa aaaatcgagc ggccggagct gcccctcgcg
     4021 cttctgaaac caccaggcca gcatcagcgg catggcaatt ttcaggattt cgctgggttg
     4081 gatgatcacc cccacattca gccagcgccg agcacctttt ttggtaatgc caaagaccgc
     4141 taccgcaatc agcaaggcaa cccccagcac ataaaccggc acggccaatg ccatcagacg
     4201 ctgcggcggt atttgcgcca ctgtgaacat gatgaaaccg gcgatcagca tgttgcggcc
     4261 atggtcttca aaacggctgc catggtcata acccgacgaa tacatcgcca tcaaaccggc
     4321 gcacgccagc aaaaacaccg caaacgccag cggaccgtca aaaccggtga accagggcga
     4381 caggcgttgc caggcagaag gacgttcaaa aaccaaagac atagaacttg attatcggca
     4441 ccctgatgtg acgcgcagtg gaatggtcac cggaccatca ttgatcaggt gcacctgcat
     4501 atcggcccca aactcacccg tttgcaccac cgggtggcgc agtctggcct gcgccacaaa
     4561 atggttgtac aggtgcaaac catctgctgg cagcgcggca ccactaaagc ctggccgatt
     4621 gccgcccgat gtgtcagccg ccagcgtgaa ctggctaacc accagcagtt cacccacccg
     4681 gcccaaaccg tcgatatcct gcaggctgtg gttcatcttg ccagccgcat cattgaagat
     4741 gcgcaatttc aggatttttt ccagcaactt gtcgctttgc gcgactgtgt cgccacgctc
     4801 cgcacacaac agcaccagca agccggcccc gatgcgccca acggtctggc cggcaatgtc
     4861 aacccgcgcc aatgcggcac gctgtaataa cgccatcatg ctagattgcg gagttggacc
     4921 cgcttgaccg ggttgctggc ggcgcgtctg ggttggcgcg acgacgcagc gggctgccga
     4981 gcgcctgtcg tcctgacgga cgatcaaggc gtatcgaccc caggcgcgtt gccattggcc
     5041 cgatcgggtg tgcagcgctg tcaccccatg ggtgagcccc atcaaagctg aaaacgtcaa
     5101 tgttcatgag aaattgcaga tgcaaaccga cggtcaaccg aggaccgcag gatgatgacc
     5161 cttcgcactg gaggagcgcc gacatcgcga tcaggccagc gtgacccgtg cgaacttgcg
     5221 cttgccaact tgcagcacat aggtacccgc ccccagcttg agccccttgt cgctgaccac
     5281 gccggagtcc acccgcacgc cgccgccatc aatcaggcga ttgccttcgc cgctcgaagc
     5341 cgccagtccg gccgatttga gcagcgcgcc aatgcccagc gctgccccat ccgcccccaa
     5401 tgccaggcga atatcggcga tctcggcagg aatgccccct ttgctgcggt tgatgaagtc
     5461 ctgctcggcc gcatctgctg ctgctgcgca atgaaagcgt gtcgtgattt ccttggccaa
     5521 ggccaccttg gcctctttcg ggttgcgacc gccatccacc tcggccttga gggcggcaat
     5581 ctgcgcttca gactgaaagc taagcagcgt gtaccagcgc cacatcaaca catccgagat
     5641 gcttaaacac ttggcaaaca tggtgttggc atcttcgctg atgccgatgt agttgttctt
     5701 gctcttggac atcttctcga tgccatcgag cccttcgagc agcggcatgg tcaaaatgca
     5761 ctgcggctcc tgaccatatt cggcctgcag gtggcgcccc accagcaggt tgaacttctg
     5821 gtcggtgccg ccgagttcca ggtcgctctt gagagctacc gagtcataac cctgcatcag
     5881 ggggtacaag aactcatgca cgctgatagg cgtaccggat tgaaaccggt catggaagtc
     5941 gttgcgctcc atcatccgcg ccaccgtata gcgcgctgcc aacccgatca tgccgcgcgc
     6001 gccaagcgcg tcgccccact cgctgttgta gcgaatctcg gtcttgttcg ggtccagcac
     6061 catcgaggcc tggcggtaat aggtctgcgc attgacctcg atctgctcgc ggctcaacgg
     6121 tggccgggtg ctgttgcgcc cggacgggtc accaatcagg gtggtgaagt cgccaatcag
     6181 gaaaatcacc gtgtg
//
LOCUS       DAOSLL010000238         6189 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11467_length_6189_cov_4.957450, whole genome shotgun sequence.
ACCESSION   DAOSLL010000238 DAOSLL010000000
VERSION     DAOSLL010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11467_length_6189_cov_4.957450"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLB25_11195"
     CDS             <1..396
                     /locus_tag="PLB25_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HOE42176.1"
                     /translation="HRHAPRFAGVQEQWVSAGIGLGIGFYDGFFGPGTGSFLVFLFVR
                     LLGYDFLNASAAAKLVNTATNVSALALFVGKGHIWWHFVFVMALANVLGSWLGTLMAL
                     RHGTGFVRAVFLLVVSALILKTGADAFLR"
     gene            539..847
                     /locus_tag="PLB25_11200"
     CDS             539..847
                     /locus_tag="PLB25_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42177.1"
                     /translation="MVWFEYAAGINAQLNQAMDQLLKTQPRLGDADLSNLYQLYVVEV
                     DPNAMQRIGSELQRVMQPLSQKASAAGGQAGEFGMQLETLVAGLLGASDMLVTPSSPR
                     "
     gene            934..1185
                     /locus_tag="PLB25_11205"
     CDS             934..1185
                     /locus_tag="PLB25_11205"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE42178.1"
                     /translation="MESTRVRDESQHDARTQVLNRKGFDLKLAQLLERKPTAGGSHWL
                     IMFDLDHFKKVNDTHGHVMGDPVLQALGEVLKGCLRASG"
     gene            1272..1595
                     /locus_tag="PLB25_11210"
     CDS             1272..1595
                     /locus_tag="PLB25_11210"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE42179.1"
                     /translation="MRAYPNVDAARYGGEEFAALMPDSSKDECSQLAELIRMRTKTLR
                     IRDRRTQAEVLTVTVSGGISAMQAGGDRPTLTARADAALYQSTQSGRDRPLPARACAG
                     HRAGL"
     gene            complement(1554..3581)
                     /gene="mrdA"
                     /locus_tag="PLB25_11215"
     CDS             complement(1554..3581)
                     /gene="mrdA"
                     /locus_tag="PLB25_11215"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOE42180.1"
                     /translation="MTVIRNVQAELSRFRLRVLVASVAVVIGFALVAVRLSYLQVIRH
                     EDLRAQAENNRTAIVPIVPNRGLILDRNGIVLATNYSAYTLEVTPSKVSNLEQTIDEL
                     SRVLDITARDRRHFKKLREEARNFESLPLRSRLSDAEVATFAAQRYRFPGVDIKARLF
                     RNYPYGELASHVIGYIGRINTAEKEALDESDDPANYRGTEYIGKLGVEQSFEAQLHGI
                     TGVDAMETSAGGRATRRLASQPSTPGNTIKLSIDIKLQKLIEDMYGDRRGALVALDPK
                     SGEVLAFVSKPTFDPNLFVDGIDQENWQLLNESIDKPLLNRALRGTYPPGSTFKPFMA
                     MAALQTNTRSATTLINDPGFYMFGGHRFGSPENERGGIMDMRRAIVESSNAYFYSLAN
                     ELGVDTMHDFMKPLGFGQITGIDINGEVRGVLPSQAWKRSYFKRPEQQKWYAGETISL
                     GIGQGYNSFTMLQLAQATATIANNGIKHTPHLVIATQDASTRVSVPVVPQPAQNLGFK
                     QANVDIIRQAMVGVTQQGTSTRVFAGAGYLSGGKTGTAQAVSLGKNERYNAARMEERH
                     RDHALYIAFAPADDPKIALAVIVENAGFGSASAAPIARRTFDYWLLGQYPNPIDLAAV
                     SKGQASAPIGKPDLAADVAWPVAAAASSASAPVPTGSASKTSAVASARTRR"
     gene            complement(3588..4118)
                     /gene="mreD"
                     /locus_tag="PLB25_11220"
     CDS             complement(3588..4118)
                     /gene="mreD"
                     /locus_tag="PLB25_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HOE42181.1"
                     /translation="MMMPRGQQLLLPARPLFIWGSLLTALALDMLQNMGLWGRAAWLP
                     DLLALALLFWTIHQPQRIGMTAAFVLGLVMDVHQSSLLGQHALAYTVLSFLAIGIHRR
                     LLWFSVPSQAAQVVPLFAVAHALELSVRLLAGDEFPGWSIILAPVFEALLWPVVSVLL
                     LVPQKRAPNPDANRPL"
     gene            complement(4115..5029)
                     /gene="mreC"
                     /locus_tag="PLB25_11225"
     CDS             complement(4115..5029)
                     /gene="mreC"
                     /locus_tag="PLB25_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HOE42182.1"
                     /translation="MPLGTLDGTPPPFFRQGPSALSKLVFFSLLAILLVVSDLRFSVV
                     RPIRSTVATVLYPIQWLMMQPIRLIQSGGQYFESLTASQAKEAAAHYQLGLQSQRALQ
                     VEQLSLENSRLRALLNMRERLNAPALAAQVLYDAADPYSRKVIIDKGALAAVQAGAPV
                     VDETGILGQITRVYPLVSEVTLVTDRNQAIPVLSVRTGARGLAFGDSTVRSGMLELRF
                     MDANVDMQVGDLLTTSGVDGVYPPGLPVARVAKVERRADSAFARIICEPVSQVTGSLH
                     VLVLQAMTGQMPSRPEPAPALSAKGGKP"
     gene            complement(5045..6088)
                     /locus_tag="PLB25_11230"
     CDS             complement(5045..6088)
                     /locus_tag="PLB25_11230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005798130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HOE42183.1"
                     /translation="MFGAFRQYFSTDLAIDLGIANTLIYVRDKGIVLDEPSVVAIRHE
                     GGPQGKKTIQAVGKEAKAMLGKVPGNIEAIRPMKDGVIADFTVTEQMLKQFIKMVHPR
                     SIFRPSPRIIICVPCGSTQVERRAIRESALGAGASDVYLIEEPMAAAIGAGLPVSEAS
                     GSMVVDIGGGTTEVGVISLGGMVYKGSVRVGGDKFDEAIINYIRRNYGMLIGEPTAEA
                     IKKNIGSAFPGSEVREMEVRGRNLSEGVPRSFTISSNEILEALTDPINNIVSAVKNAL
                     EQTPPELGADIADRGMMLTGGGALLRDLDRLLAEETGLPVLVAEDPLTCVVRGCGIAL
                     EQMELMVSIFTTE"
BASE COUNT         1285 a         1797 c         1821 g         1286 t
ORIGIN      
        1 catcggcatg caccgcgctt tgccggagtc caagagcagt gggtcagtgc tggcattggc
       61 ctgggcatcg ggttttacga cggttttttt ggtccgggaa ctggcagttt tctggttttc
      121 ttgtttgtgc gcttgctggg gtatgatttt ttgaacgcat ctgctgcggc caaactggtc
      181 aataccgcca ccaatgtttc ggcactggcc ctgtttgtcg gcaagggtca catctggtgg
      241 cattttgtct ttgtcatggc gttggccaat gtgttgggca gttggctcgg aacgctcatg
      301 gcactcaggc atggcaccgg ttttgtgcgg gcggttttcc tgctggtagt gagcgccctg
      361 attctgaaaa ccggcgctga tgcctttttg cgctagtacc gcatgatttg cccaatacaa
      421 gttttgtcat gaaaagagtg cttcgctact ccctatccaa ggagaaaagt accgagttgc
      481 tgcgcatcgt acttgggcgc ctgggtcagc acgacgcggc ctgcaatccc ctgacgtcat
      541 ggtctggttt gagtacgctg cgggcatcaa tgcccagctc aaccaggcaa tggaccaact
      601 cctgaagacc caacctcggc tgggcgatgc tgatctgtcc aacctgtacc agttgtatgt
      661 ggtcgaagtc gacccgaacg cgatgcaacg cattggcagc gagttgcaac gggtcatgca
      721 gccgctcagc caaaaggcct ctgcggccgg tggccaggct ggcgaatttg gcatgcaact
      781 tgaaacgctg gtggccggac tgctgggtgc cagcgacatg ctggtgaccc cgtcgtcacc
      841 cagatgattg aaagcagcgc ccgcaagcgc agttcgaccc aggccctggc gtccgaggtg
      901 acaagcaagc agcttgaaat accgcgcctg taaatcgagt caacccgggt acgcgatgag
      961 tcgcagcatg atgcccggac ccaggtgctc aaccgcaagg ggtttgattt gaaacttgcg
     1021 cagttgctgg aacgcaaacc cacggcgggt ggctcgcact ggctcatcat gtttgacttg
     1081 gaccacttca aaaaagtcaa cgacacccat ggtcatgtga tgggagaccc ggtactgcag
     1141 gcactcggcg aggtgctcaa gggctgcctg cgtgcctcag ggtaaatttg ttcttatggc
     1201 atcttcgtca ttcccgcctg cgcgggaatg acggtaaatc aacgacttag cgaaagaaca
     1261 agtttgccct gctgcgggcc tatccaaacg ttgacgcggc gcgttacggc ggtgaagaat
     1321 ttgctgcgct gatgcccgac agctcgaagg atgagtgcag ccagcttgcc gagttgatcc
     1381 gcatgcgaac caaaactttg agaattcgtg atcgccgcac ccaggcggag gtgctgacgg
     1441 ttaccgtgtc tggtggcatc tcggccatgc aggcgggcgg tgatcggcca actctgacag
     1501 cgcgtgccga cgcggcgctg taccaatcca cgcaaagtgg tcgtgaccgc cccctacctg
     1561 cgcgtgcgtg cgctggccac cgcgctggtc tttgatgcgc tgccagtcgg aactggtgcg
     1621 gatgcagagg aagctgccgc agcgacaggc caggcaacgt cggcggccag atcaggcttg
     1681 ccaattggcg cgctcgcctg ccccttgctc acggcggcca ggtcaatcgg gttggggtat
     1741 tgacccagca gccagtagtc aaaggtgcgc cgggcaatcg gggccgccga agccgagcca
     1801 aagccggcat tttccacaat cacggccagc gcaatcttgg ggtcatcggc cggcgcaaag
     1861 gcaatgtaca gagcgtggtc gcggtggcgc tcctccattc gcgcggcgtt atatctttcg
     1921 tttttgccca gggaaactgc ttgcgctgtg ccagtcttgc cgccgctcag gtagccggca
     1981 ccggcaaaca cccgggttga cgtaccctgc tgggttacgc ccaccatagc ctggcggatg
     2041 atgtcaacat tggcttgctt gaagcccagg ttttgagccg gctgcggcac cacaggaacg
     2101 ctgacacggg tgctggcatc ttgcgtggca atcaccagat ggggggtgtg cttgatgcca
     2161 ttattggcga tggtggcggt agcctgggcc aactgcagca tggtaaagct gttatatcct
     2221 tgtccaatgc ctagtgaaat ggtttcaccg gcgtaccact tttgctgttc cgggcgcttg
     2281 aaatagctgc gcttccaggc ctggctgggc aacacaccgc gtacttcacc gttgatgtca
     2341 atcccggtga tctggccaaa gcccagcggt ttcatgaaat cgtgcatggt gtccacgccc
     2401 aactcgttgg ccagtgaata aaagtaggcg ttgctggact cgacaatggc gcggcgcatg
     2461 tccatgatgc cgccgcgctc gttttccggg ctgccaaaac ggtggccacc aaacatgtaa
     2521 aagccaggat cgttgatcaa ggtggtggcc gaacgggtgt tggtctgtag cgccgccatg
     2581 gccataaagg gtttgaatgt ggagccaggc ggataggtgc cgcgcagcgc gcggttgagc
     2641 aatggcttgt cgatcgactc attgagcaat tgccaatttt cctggtcgat gccatcgaca
     2701 aacagattgg gatcgaaagt gggcttgctg acaaacgcca gcacttcacc agacttgggg
     2761 tcgagcgcca ccaatgcccc gcgccgatcg ccatacatgt cttcaatcag cttttgcagc
     2821 ttgatgtcga ttgacagttt gatggtgttg cccggtgtgg acggctggct tgccagccgt
     2881 cgtgtggcgc gcccgccagc cgatgtttcc attgcatcga caccggtgat gccgtgcaat
     2941 tgagcttcaa agctctgctc gacgccaagc ttgccgatgt actctgtgcc acggtagttg
     3001 gccggatcgt ccgattcgtc aagggcttcc ttttctgctg tgttgatacg cccgatgtag
     3061 cctatgacat ggctggccaa ttcaccgtaa gggtagttgc gaaacaatct ggccttgata
     3121 tcgaccccgg gaaagcgata gcgctgggcg gcaaaggtgg caacttccgc gtcactcaag
     3181 cggctgcgca gcggtagtga ttcaaagttt ctggcttctt cccgcagctt cttgaaatgc
     3241 cggcgatcac gtgccgtgat gtcaagaacc cgcgacagct catcgatggt ctgctccaga
     3301 ttgctcacct tggagggggt cacttccagc gtataagccg aatagttggt ggccagcaca
     3361 atgccattgc ggtccaggat gagcccacgg ttgggcacaa tcggcacaat ggcggtgcgg
     3421 ttgttttcag cttgcgcgcg caggtcctca tgccggatca cctgcaggta actcaagcgc
     3481 acagcgacca atgcaaagcc gatcacaacg gccacgctgg ccaccagcac gcgcaggcga
     3541 aagcgcgaca gttcagcctg aacattgcgg atgaccgtca tggggagtca gagcggtcga
     3601 ttggcatcgg ggttgggggc ccgcttttgt ggcaccagca acagcacact gaccactggc
     3661 cacaagaggg cctcaaagac cggggccaga ataatcgacc agccaggaaa ttcatcacct
     3721 gccaacaggc gcacagaaag ttccagtgcg tgcgcgacag caaacagcgg caccacttgc
     3781 gccgcctggc tcggaacact gaaccagagc aggcgccgat ggatgccaat tgccagaaaa
     3841 ctcagtacgg tgtaggccag tgcatgttgc cccaggaggg aactttggtg cacatccatg
     3901 accagcccca gcacaaacgc agccgtcatg ccgatgcgtt gcggctgatg gatggtccaa
     3961 aagagcaaag ccaacgccag caagtcaggt agccaggcgg cacgacccca taggcccatg
     4021 ttttgcagca tgtccagcgc cagcgcagtc aaaagactgc cccagatgaa cagtggcctg
     4081 gccggcagca gcaactgctg gccgcgcggc atcatcatgg cttgcctcct ttggccgaca
     4141 gtgctggcgc cggttcggga cgacttggca tttgccccgt catggcttgc agcaccagca
     4201 catgcaggct gccggtaacc tgcgaaaccg gttcacaaat gatgcgtgca aaggcggagt
     4261 ccgcccgacg ctcaaccttg gccacgcgag ccaccggaag acctggtgga taaacaccat
     4321 caacaccgct ggtggtcagc aagtccccga cctgcatatc gacgttggca tccatgaacc
     4381 gaagctccag catgccggag cgcaccgtcg agtcgccaaa cgccagaccg cgtgcgccag
     4441 tgcgaacgct caggaccgga atggcctggt tgcggtcggt caccaatgtc acctcgctga
     4501 ccagtgggta aacccgggtg atttgcccca gaatacctgt ctcgtcaaca actggtgcac
     4561 cggcctgtac agccgccagc gcgcctttgt cgatgatcac ttttcgactg taggggtcgg
     4621 ctgcgtcata caggacctga gccgccagag ccggggcatt gaggcgctcg cgcatgttga
     4681 ggagggcacg caggcggctg ttctcaaggc tgagttgctc cacctgcaag gcacgctgcg
     4741 actgcagacc gagttgataa tgggctgccg cttccttggc ctgcgaagct gtcagtgact
     4801 caaaatactg gccgccgctt tgtatcagtc tgatcggttg catcatcagc cactgtatcg
     4861 ggtacagcac ggttgcgacg gttgaacgga ttggccggac aacgctaaag cgcaggtccg
     4921 acaccaccaa caaaatcgcc agtaagctga aaaataccag tttggaaagt gctgacggac
     4981 cctgcctgaa aaacggtggc ggagttccgt ccagcgtacc cagaggcatc gctgctccag
     5041 tcgcctattc ggtggtaaaa atggagacca taagctccat ctgctccagc gcaatgccac
     5101 agccacgcac cacgcaagtc agcgggtctt cagccaccaa taccggcagg ccggtctctt
     5161 cggccagcag gcggtcgagg tcacgcagca gcgcgccacc gccggtcagc atcatgcctc
     5221 ggtcggcgat gtcagcaccg agttcgggcg gggtttgctc cagtgcgttc ttgacggccg
     5281 agacaatgtt gttgatcggg tccgtcagcg cttccaggat ttcgttgctg gaaatggtga
     5341 aactgcgcgg cacgccttcg gacaggttgc ggccacgaac ttccatctcg cgcacttcgc
     5401 tgcccggaaa ggcagagccg atgttcttct tgatggcttc agcggtgggc tcaccaatca
     5461 gcatgccgta attgcgtcga atatagttaa tgatggcttc gtcaaacttg tcaccgccca
     5521 cccgcacgct acccttgtaa accatgcctc ccagagaaat gacaccgacc tccgtggtgc
     5581 cgccaccaat gtctaccacc attgagccac tggcttcact gacaggcaaa cctgcaccga
     5641 tggctgcggc catgggctct tcgatcaaat acacatcact ggcaccggca cccagtgcac
     5701 tttcgcgaat cgcgcggcgc tccacctggg tcgaaccaca gggtacacag atgatgatgc
     5761 gggggctagg ccgaaagatt gaccgtgggt gcaccatctt gatgaactgc ttgagcattt
     5821 gttctgtgac ggtaaagtcg gcgatcacgc catctttcat tgggcgaatg gcctcgatat
     5881 tgcctggcac cttgcccagc atggccttgg cttctttgcc gaccgcctga atcgtctttt
     5941 tgccttgtgg accgccttcg tgccggatcg cgaccaccga gggctcatca agcacgatac
     6001 ccttgtcacg gacataaatc agagtgttgg caatgccaag gtcaatagcc aggtcagtag
     6061 aaaaatactg acgaaatgct ccaaacatga ataattcctc tcgggcacag gagcgcttcg
     6121 gcccgcatgt caccctgtaa tgtgttcaaa caagtgattt ttcggcaata aaccatgccg
     6181 cttcaggct
//
LOCUS       DAOSLL010000239         6174 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11508_length_6174_cov_4.001961, whole genome shotgun sequence.
ACCESSION   DAOSLL010000239 DAOSLL010000000
VERSION     DAOSLL010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11508_length_6174_cov_4.001961"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="PLB25_11235"
     CDS             complement(<1..1091)
                     /locus_tag="PLB25_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HOE42184.1"
                     /translation="MIAPVDVSFFPRAAKPLNGYRKFWAARFGTAAFLPMSRAEMDLL
                     GWDSCDIVLVTGDAYVDHPSFGMAVIGRMLESQGFRVGIIAQPDWHSAEPFRALGKPN
                     LFWGVTAGNMDSMINRYTAERKIRSDDAYTPGDVAGRRPDRAAIVYSQRCREAFKDVP
                     VVLGGIEGSLRRIAHYDYWSDKVRRSIVVDAKCDLLLYGNAERAIVEIAHRLSQREPI
                     EQITDVRGTAFVRRSDDASRLGWFEIDSTDVDQPGPIESHINPYQTTAEQATGQGSTC
                     ARQDDAESVQCATDVIAASAYSTGSGGLKGLKVAATGRDCDQTGVSAESPPQPAAQPL
                     QFMPSPALPMRIGRRLLPARDRSVIRLPS"
     gene            complement(1366..1566)
                     /locus_tag="PLB25_11240"
     CDS             complement(1366..1566)
                     /locus_tag="PLB25_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative motility protein"
                     /protein_id="HOE42185.1"
                     /translation="MNISNTPSVQSATTAAQAQSADAVNMLVLKKALNTQAAAAITLL
                     QALPQPPLASQGTVGTRLNTFA"
     gene            complement(1603..2748)
                     /locus_tag="PLB25_11245"
     CDS             complement(1603..2748)
                     /locus_tag="PLB25_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HOE42186.1"
                     /translation="MSNPLFPPFDPDQALQVALIGYGSAAKVFHAPLISGVAGLALAC
                     ISSSRPDDVLRDWPQVRVVAAPQQAFEDAAIDLVVIATPNDSHYQLALAALQAGKHVV
                     VDKPCTVTLAETEHLLAVAAQRGRVLTVFQNRRFDADFLALQQVLASGALGRVVQMAS
                     HFDRYRPVVPRRWREQNQPGSGLWVDLGPHLVDQALVLWGMPDAISLDLACLRDGAQV
                     NDWFHAVLRYDSAHAGLRVILHASTLVAETGPRWAVHGTLGSFTKFGLDGQEDALKTG
                     QRPQLDALANWGVDPLPGQVLCMESIAGAAAPVSVRQVAPHPAGNYLNYYANLRDHLR
                     GQADLLVSPQQVRQVMLVLTLGEQSALQGRVVPSGNPEGLRLSRSGR"
     gene            2870..3793
                     /locus_tag="PLB25_11250"
     CDS             2870..3793
                     /locus_tag="PLB25_11250"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HOE42187.1"
                     /translation="MLEFLPAPLVGCIACTLMLLNILFWVPVLLLVSLVKLLLPFKAV
                     RLLIDPLLLRIAEAWIAGNSGWMRLTQRMDWQVSGIDALSPRQWYLVVCNHQSWVDIL
                     VLQHVLNRRIPLLKFFLKKELIWVPIMGLAWWALEFPFMRRRSEAYLQQHPEERGKDA
                     ATTRAACEKYALVPTSVMNFLEGTRCTPAKQQRQQSPYRHLLKPKAGGITLALDAMGD
                     KFGAVLDITLVYPAGTPNFWQFLQGRVPRVLVLARTLPVPQAAPQSGQGSDVALRETC
                     QQWVNQLWQVKDAQISAVLASAHAGDPLVGH"
     gene            4023..>6174
                     /locus_tag="PLB25_11255"
     CDS             4023..>6174
                     /locus_tag="PLB25_11255"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HOE42188.1"
                     /translation="MIKSARRLVYVNLMGATLAVMVGTSDCYAGAGISTDGSLGALQT
                     VAGANAGAGNFLIPQSLGALVGNNLFHSFSTFNIDTGQSATFATATPSIGNVISRVTG
                     GSVSQINGQLRLTSAGGAPNFFFINPAGVAFGAGGSIDMPGAFHVSTADYVKFVNGDK
                     FYADLSRTSALSSAAPEAFGFLGTSRAAITVKDGAILATKSFHPISIAGGDIELNNGT
                     VATQGGDIRVVTLGQKEQEIGFTGALPMVEGNLAILNRSKIASSTASSGNAGTVKISA
                     GAIAMDGQGKGAVIASFTVGNAANDAKTGDAGSIDITASGAISIVDGGQIYSSTDSSG
                     KVGTVLIRANSLTVDGQGGQPTGIASQAVSNAANGGEAGDAGSLDITASGALSIVNGA
                     LISSITESTGNGGAVKVSAGSLAIDGQGKVTGIVGQALGDAVNHGKTGDAGRLDIMVH
                     GALSIVNSGQITSNTFSSGKAGAVKISADSVAIDGGQGINWTGIGSNAVSNAANAGKT
                     GDAGSLDITVSGALSIVNGSEISSSTYSFGNAGAVKVSAGSIVVDGQGSEWFTGIHNQ
                     AISDAANGGKTGDAGSLDITVSGALSIFNGGTVSSSTYSFGNAGNVKVSAGSIAIDGQ
                     GNGAGISSNANGNADNGGKTGDAGSIDIMTNGALSIVNGGQISSSTFSVGEAGTVKVS
                     AGSITIDRQGGERTTGIVSNAVGDTANGGKAGDAG"
BASE COUNT         1238 a         1853 c         1829 g         1254 t
ORIGIN      
        1 aagctcggca agcgaatcac cgaccggtcc cgcgctggca gcagccgccg gccaatgcgc
       61 atcggcaagg ccgggcttgg catgaactgc aagggttggg cagcaggctg gggcggcgac
      121 tcagcactga cgccagtctg atcgcagtcg cgccctgtgg cggcaacttt caagcctttt
      181 aaccctccag agcccgtcga atatgcgctg gcagctatta catccgtagc acactggacg
      241 ctctcggcat cgtcttgccg ggcacaagtg ctgccttgcc cggttgcctg ctcagcggtg
      301 gtctggtaag gattgatgtg agactcaatc gggccgggct gatccacatc ggtcgaatcg
      361 atctcgaacc agccaagccg actggcatcg tcagagcggc gcacaaaggc cgtgccccgc
      421 acatcggtga tctgctcaat cggctcgcgc tggctcagcc ggtgcgcaat ttcaacgatc
      481 gcccgctcgg cattgccgta cagcagcaga tcacacttgg catccaccac gatgctgcgg
      541 cgcaccttgt cgctccagta gtcgtaatgg gcgatgcggc gcaggctgcc ctcaatgccg
      601 ccgagcacaa ccggcacgtc cttgaacgcc tcgcggcagc gctggctgta gacaatcgcc
      661 gcgcggtcgg gccgccgacc agcaacgtca cccggcgtgt aggcatcgtc gctgcggatt
      721 ttgcgctctg cggtgtagcg gttgatcatc gaatccatgt tgcccgccgt cacaccccag
      781 aacagattgg gtttgcccaa agcccgaaat ggctcggcgc tgtgccagtc cggctgggcg
      841 atgatgccga cccgaaaacc ttgcgattcg agcatgcggc caatcactgc catgccaaaa
      901 ctcgggtgat ccacatacgc atcaccggtt accagcacga tgtcacaact gtcccagccc
      961 agtaggtcca tttcggcgcg gctcattggc aaaaatgccg cggtgccaaa acgcgccgcc
     1021 caaaatttgc gatagccgtt caagggcttg gctgctcgcg gaaaaaaaga cacgtcaaca
     1081 ggggcgatca tgcaaggctc ttggggtgga aaggcccgca gtgtaaggtt ttgcgggttt
     1141 cttcggcctt tggcaaggcc ttgccggacc gacggctttg tgtttttcta cccaggtcgg
     1201 accgccaacg gcccatgaac aggcgcttgc tacaaaaggg gctctgcccg cgattcaatc
     1261 agaaaccgaa ccagcgatgc agcaacggcg tgaatcgcgc ccggtcaaaa agggccgtgg
     1321 ctcggcaaaa agccgacttg tcaagcctga tcaagcttgt actgatcagg caaaggtatt
     1381 gagcctggtg ccaaccgtac cctggctggc cagcggcggc tgcggtaggg cttgcagcag
     1441 cgtgatcgcc gccgcagcct gggtgttgag tgcctttttc aacaccagca tgttgacggc
     1501 atcagcagac tgtgcttgcg ccgccgtggt ggcggattga acggatgggg tattggagat
     1561 attcatggca ggttttgcag tggtctgatc aggctcaacc ttttatcgac cgctgcgact
     1621 caaccttaag ccctcggggt tgccacttgg caccacgcgc ccttgcaacg cactctgctc
     1681 gcccagggtc agcaccagca tcacttggcg aacctgctgc gggctcacca gcaaatctgc
     1741 ctggccgcgc aaatggtcgc gcagattggc gtagtagttc aaatagttgc ccgccggatg
     1801 aggcgcaacc tggcgcaccg acaccggcgc tgccgcaccc gcgatgcttt ccatgcacag
     1861 cacctgaccc ggcagcgggt ctacacccca attggccaag gcatcaagct gtggccgctg
     1921 gccggttttc aacgcatctt cttgcccatc aagcccgaat ttggtgaaac tgcccagggt
     1981 gccatgcacc gcccagcgcg gaccagtctc ggccaccagc gtgctggcgt gcagaatcac
     2041 ccgcagaccg gcatgtgcgc tgtcatagcg cagcaccgca tgaaaccagt cgttcacctg
     2101 cgcgccatcg cgcagacagg ccaggtccag actgattgca tcgggcatgc cccacagcac
     2161 cagagcctga tcaaccaggt gcgggcccag atcgacccac agacccgagc caggctggtt
     2221 ttgttcgcgc cagcgcctgg gcacgactgg gcggtaacgg tcaaagtgcg acgccatctg
     2281 caccacccgg cccagcgcac cactggcaag cacctgttgc aaggccagaa agtcggcatc
     2341 gaaacgccgg ttctgaaaca ccgtcagcac cctgccccgt tgcgccgcaa ccgccagcag
     2401 atgctcggtt tcggccagcg tcacggtaca gggtttgtcc accaccacat gtttgcccgc
     2461 ctgcagcgca gccagcgcca actggtagtg gctgtcgttg ggggtggcga tcaccaccag
     2521 gtcaatcgcc gcatcttcaa acgcctgctg cggtgccgcc accacccgga cctgtggcca
     2581 gtcgcgcaac acatcgtccg ggcggcttga actgatgcag gccagcgcca gcccggcaac
     2641 accggaaatc agcggggcgt gaaacacctt ggccgcgctg ccatagccaa tcagcgcgac
     2701 ttgcagcgcc tggtccgggt caaaaggggg aaaaagcggg ttgctcatgg gttgatcctg
     2761 cgggttgccg aatggtctga ccgttgggcg gtggcttgca cgttgcccag cttggttcaa
     2821 aatgctgcac tggttcagtc ttgttgcctt tttaccgtcg gattttgtca tgcttgaatt
     2881 tctgcccgcg ccgctggtcg gatgcatcgc ttgcacgctg atgttgctca acattctttt
     2941 ttgggtgccg gtgttgctgc tggtgtcgct ggtcaagttg ctgctgccct tcaaggccgt
     3001 gcggctgctg atcgatccgc tgctgctgcg catcgccgaa gcctggattg ccggcaacag
     3061 cggctggatg cgtctgaccc agcggatgga ctggcaggtc agcggcatcg acgcgttaag
     3121 cccgcgccag tggtatctgg tagtctgcaa ccaccagtcc tgggttgaca ttctggtgct
     3181 gcagcatgtg ctgaaccggc gcattccatt gctcaagttt tttctgaaaa aggaactgat
     3241 ctgggtgccc atcatgggcc tggcctggtg ggcgctggag tttcccttca tgcgccgacg
     3301 cagcgaagcc tacctgcagc aacaccccga agagcgtggc aaagatgccg ccaccacccg
     3361 tgccgcctgc gaaaaatacg ccctggtgcc caccagcgtg atgaatttcc tggagggcac
     3421 gcgttgcacc ccagccaaac aacagcgcca gcagtcgccc taccgccatc tgctcaagcc
     3481 caaggccggc ggcatcacgc tggcgcttga tgcgatgggc gacaagtttg gcgcggtgct
     3541 agacatcacg ctggtctatc cggctggcac gcccaacttc tggcagtttt tgcaagggcg
     3601 ggtgcccagg gtgctggtgc tggcccgcac tttgccagtg ccgcaggcag cgccccaaag
     3661 cggtcagggc agcgacgtgg ccctgcgcga aacctgccag cagtgggtca accagttgtg
     3721 gcaggtcaag gatgcgcaaa tttctgctgt cttggcaagc gcgcacgccg gtgacccgct
     3781 ggtcggtcac tgaggcgatg gtttttcgct aaaacctctc tgtcccttat ggtgattgca
     3841 cttacagcta tatttatgat agctaggtaa taccttgaca ggttttgacg tggtgcgtga
     3901 aagacccgcg acatcggttc gtcgggtgat caccctgatg cgctgcttga atgtaccaac
     3961 cgccctcgaa atctggttaa agtgttgtct ggtttgtatt gaccgcacct gcaaggagca
     4021 tcatgatcaa gtcagcaaga cgtttggtat acgtcaacct catgggtgcg acattggctg
     4081 tgatggtggg cacatccgac tgctatgcag gtgctggcat ctcgacagac ggcagcctcg
     4141 gtgccctcca gaccgttgcc ggagccaacg caggtgcggg gaacttcttg attccgcaat
     4201 cgctcggggc acttgtcggc aataaccttt tccacagttt ctcgaccttc aatatcgaca
     4261 cggggcaatc tgcgacgttt gccaccgcca cgccatcgat tggcaacgtc atcagccggg
     4321 tcacaggcgg cagtgtgtcc cagatcaatg ggcaactcag gctcacttcg gctggtggcg
     4381 cgcccaattt tttctttatc aacccggcag gtgtcgcgtt tggtgccggt ggctcgattg
     4441 atatgcccgg cgcgtttcat gtcagcactg cagattacgt caagttcgtc aatggcgaca
     4501 agttctacgc tgacttgtca agaaccagtg ccttgtcgag tgcggcaccc gaagcatttg
     4561 ggtttctggg aacgtcgcgg gccgccatca ccgtcaagga tggtgcgata ttggcgacaa
     4621 aatccttcca cccgatcagc atcgcaggcg gcgacattga actcaacaac ggcacagttg
     4681 ctacacaggg cggggatatt cgtgtagtga cgctgggcca gaaagagcag gaaatcggat
     4741 tcactggcgc cttgccaatg gttgagggaa atcttgccat cttgaatcgc agcaagattg
     4801 cctccagcac cgcttcctct ggcaatgctg gtaccgtcaa aatcagcgct ggcgctatcg
     4861 ccatggatgg tcagggcaag ggagcagtca tcgccagctt taccgtcggc aatgctgcca
     4921 atgacgccaa aacgggtgat gctggcagca tcgacatcac ggccagcggt gcgatttcca
     4981 tcgtcgatgg tggacaaata tattccagta ccgactcctc cggcaaggtt ggcacggtct
     5041 tgatcagggc aaactctcta actgttgatg gtcagggcgg ccagccgaca ggcattgcca
     5101 gccaggcagt cagcaacgct gcgaacggcg gtgaagcggg cgatgctggc agtctcgaca
     5161 tcacggccag cggtgcgctg tccatcgtca atggtgcgtt gatatcctcc attaccgaat
     5221 ccactggcaa tggtggcgcg gtcaaggtca gcgctggctc tctcgccatt gatggtcagg
     5281 gcaaggtgac aggcattgtc ggtcaggcac tcggcgatgc tgtcaaccac ggaaaaacgg
     5341 gcgatgctgg ccgtctcgac atcatggtcc atggggcgct ttcgatcgtc aatagtggac
     5401 aaataacttc caatactttc tcctccggca aggctggcgc ggtcaagatc agtgctgact
     5461 ctgtcgccat tgatggtggt cagggcatca actggacagg cattggcagc aatgcggtca
     5521 gcaatgctgc caatgccggt aaaacgggcg atgcaggcag tctcgacatc actgtcagcg
     5581 gcgcgctttc catcgtcaat ggtagcgaaa tatcttccag tacctactcc tttggcaatg
     5641 ctggcgcggt caaggtcagc gctggctcta tcgttgttga tggtcaggga agcgagtggt
     5701 tcacaggcat ccacaaccag gctataagcg atgccgccaa tggcggcaaa acgggcgatg
     5761 ctggcagtct agacatcacc gtcagcggtg cgctttccat cttcaatggc ggcacagtat
     5821 cttccagtac ctactccttt ggcaatgctg gcaacgtcaa ggtcagcgct ggctctatcg
     5881 ccattgatgg tcagggcaac ggagccggca tttccagcaa tgcgaatggc aacgctgaca
     5941 atggcggaaa gacgggcgat gctggcagta tcgacatcat gaccaacggt gcgctttcca
     6001 ttgtcaatgg tggacaaatt tcctccagta ccttctccgt tggcgaggct ggcactgtca
     6061 aggtcagcgc gggctccatc accattgatc gtcagggcgg cgaacggaca acaggcattg
     6121 tcagcaatgc ggtcggtgac actgccaacg gcggtaaagc gggcgatgca ggca
//
LOCUS       DAOSLL010000240         6135 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11586_length_6135_cov_5.266118, whole genome shotgun sequence.
ACCESSION   DAOSLL010000240 DAOSLL010000000
VERSION     DAOSLL010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6135
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11586_length_6135_cov_5.266118"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="PLB25_11260"
     CDS             <1..386
                     /locus_tag="PLB25_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HOE42189.1"
                     /translation="MTAMLLEATEAGVAGAGAHFHHQVLIDSARVHSGLIASGDRFVS
                     KAEDAASVLADLQAAGFLPLAIEMEGAAVAQVCRDYGLPFAAVRTISDRADDQAHVDF
                     SAFVNQVASPYALSIVSQFIRLLSK"
     gene            complement(427..1413)
                     /gene="corA"
                     /locus_tag="PLB25_11265"
     CDS             complement(427..1413)
                     /gene="corA"
                     /locus_tag="PLB25_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HOE42190.1"
                     /translation="MLNIFTLANGRLIQEEIESLEELSHFQPIWVDLESPTPEEKRWI
                     KQYFGLSIPEDAMDEDIEESARFYKEDNGELHIRSDFLVADDDDPHTVRVAFILNLVN
                     ANLKSLGILFSIHDEDVPVFRLLRMRARRAPGLIEDAKEVLLKLFDADAEYSADTLEE
                     IYDELEKASAKVLSGDVTDSMAGEVLGAIARHEDMNGRIRRNVMDTRRAVSFMMRSKI
                     LSGEQFEEARLILRDIDSLDSHTAFLFDKINFLMDATVGFININQNKIIKIFSVASVA
                     LLPPTLIASIYGMNFKFMPELDWKAGYPLALGLMLASALVPMWYFRKRGWLK"
     gene            1743..2072
                     /gene="raiA"
                     /locus_tag="PLB25_11270"
     CDS             1743..2072
                     /gene="raiA"
                     /locus_tag="PLB25_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOE42191.1"
                     /translation="MNLTISGHHLEVTQALRSYVTTKLDRITRHFDQVMDVKVLLTLE
                     NLKEKERRQKAECNIHLKGIELFAESAHADLYAAVDELVDKLDRQVVRHKGRLQSHHH
                     HAPKRLM"
     gene            2154..2621
                     /locus_tag="PLB25_11275"
     CDS             2154..2621
                     /locus_tag="PLB25_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOE42192.1"
                     /translation="MNRLASILTAAQVLVQVDVSSKKRAFEEAGLLFENLHGLSRALV
                     TDSLFSRERLGSTGLGHGVAIPHGRIKGLKAPMAAVFQLAKPIGFEAPDDLAVGIMIF
                     LLVPEAATQKHLEILSEIAEMLSDTNLREKLVTSPSAAALHRLISDWTSVQSL"
     gene            2661..3611
                     /gene="hprK"
                     /locus_tag="PLB25_11280"
     CDS             2661..3611
                     /gene="hprK"
                     /locus_tag="PLB25_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOE42193.1"
                     /translation="MKPTFISADVLFEAFRGSLRWEWVAGLGASERRFDEVAVRAARS
                     GADLVGYLNYIHPYRVQILGEREVAYLTNATAEDCSRRIARIVTLEPPVLILADRQVA
                     PQALMSMCERAQLPMFSTANSSAFVIDVLRAYFSKHFADRSTMHGVFMDILGLGVMIT
                     GESGLGKSELGLELISRGNGLVADDAVDLYRINQDTIEGRCPELLQNLLEVRGIGLLD
                     IRCIFGETAVRRRKRLQLIVHLVRRDTMERDYERIPYEPLTQHVLDVPVRKVVIQVVA
                     GRNISVLVEAAVRNTILQIRGVDTYKEFVERHRKAMDQAG"
     gene            complement(3699..4124)
                     /gene="fur"
                     /locus_tag="PLB25_11285"
     CDS             complement(3699..4124)
                     /gene="fur"
                     /locus_tag="PLB25_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HOE42194.1"
                     /translation="MTKIDELKSTGLKVTLPRLKILELFQKGAQRHMTAEDVYRVLLE
                     DHADVGLATVYRVLAQFEHADILTRSHFESGKAVYELNEGQHHDHLVCLDCGRVEEFY
                     DAAIERRQNAVALEKGFVISEHALSLYAHCTRATCQHRP"
     gene            4223..4768
                     /locus_tag="PLB25_11290"
     CDS             4223..4768
                     /locus_tag="PLB25_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HOE42195.1"
                     /translation="MSETPVRCVWLGSLFTVALTLGACGSFNSASTRLANVVTPYKID
                     IVQGNFVSREQAAALKEGMSRSQVRDILGTPLLTPIFHADRWDYVFTIKRQGVQAQAR
                     RVTIFFKGDALLRVEADVLPTEAEFVASLDSGRKAGKVPLLEAPEASLQATPVPPAVA
                     APKALPPLRDSYPPLEDPAAR"
     gene            4788..5597
                     /gene="dapB"
                     /locus_tag="PLB25_11295"
     CDS             4788..5597
                     /gene="dapB"
                     /locus_tag="PLB25_11295"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HOE42196.1"
                     /translation="MSNLHRICVAGATGRMGRMLIEAVWNADDCQLGGALELAGNAAM
                     GQDPAAFLGRNSGVAITADMVAGLQNSQVLIDFTRPQGTLAHLRVCRQLGVKAVIGTT
                     GFSDAEKAEIATIANDIAIVMAPNMSVGVNVTLKLLELAAQALATGYDIEIIEAHHRH
                     KVDAPSGTALKMGEVIASALGRNLNDCAVYDRHGITGERDPSSIGFATIRGGDIVGDH
                     TVLFAGTGERIEISHKSSSRVTYAQGSLRAVRFLHQQATGLFDMADVLHLK"
     gene            5594..>6135
                     /locus_tag="PLB25_11300"
     CDS             5594..>6135
                     /locus_tag="PLB25_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOE42197.1"
                     /translation="MSLAQFFWQGDMLARAVALLLLAMSVASWVVILWKTWLMQRASR
                     DVARCIAAFWQSSSIAEATQKLQSFDREALVLPMVLATNIEAGSSLAQASGKTQQLTR
                     VLRNALHAALGKLQHGQVLLATVGATAPFVGLLGTVWGIYHALIGIASAGQVTIDKIS
                     GPVGESLIMTAAGLLVAIPAV"
BASE COUNT         1242 a         1749 c         1742 g         1402 t
ORIGIN      
        1 ccatgactgc tatgctattg gaagctactg aggcaggtgt ggcgggggct ggtgcccatt
       61 ttcatcatca agtcttgata gattcagcca gggtgcacag tggcttgatt gccagcggcg
      121 accggtttgt gtcgaaagcc gaggatgccg caagtgtgct ggctgacctg caggcggcgg
      181 gttttctgcc acttgccatt gagatggaag gggctgcggt agcgcaggtt tgtcgcgatt
      241 acggcctgcc tttcgccgcc gtgcgcacca tctctgaccg ggccgatgac caggcacacg
      301 ttgatttttc agcctttgtc aaccaggttg ccagcccgta tgcactgtct atcgtcagcc
      361 agtttattcg gctgttatca aaataatagc tgctagcgct tactgggcaa gggccggagc
      421 cactttttac ttgagccagc cgcgttttct gaagtaccac atgggcacca gcgcgctggc
      481 cagcatcaga cccagcgcca gcgggtaacc agccttccag tccagttcag gcatgaattt
      541 gaagttcatg ccgtaaatgc tggcgatcag cgtgggtggc agcaacgcca cgctggccac
      601 cgaaaagatc ttgatgatct tgttctggtt gatgttgatg aaaccgacgg ttgcgtccat
      661 cagaaagttg atcttgtcaa acaagaacgc cgtgtgcgag tccagcgagt cgatgtcgcg
      721 caaaatcaga cgcgcctctt caaattgttc cccgctgagt attttgctgc gcatcatgaa
      781 actgactgcg cgacgggtgt ccatgacgtt gcggcgaatc cgcccattca tgtcttcatg
      841 ccgggcaatc gcccccagca cttcgcccgc catggaatcg gtcacatcgc ccgagagcac
      901 tttggcgctg gctttttcaa gttcatcgta aatttcttcg agcgtatcgg ccgagtattc
      961 ggcatcggca tcaaacagct tgagcagcac ctccttggca tcttcgatca ggcctggtgc
     1021 ccggcgtgcc cgcatgcgca gcagtcgaaa taccggcacg tcttcgtcat ggatggaaaa
     1081 aagaatgccc aggcttttca ggttggcatt caccagattc aagatgaagg ccactcgaac
     1141 tgtatgcggg tcatcgtcgt cggctaccaa aaagtcgctg cggatgtgca attcaccgtt
     1201 gtcttccttg tagaagcggg cagactcttc aatgtcttcg tccatcgcgt cttccgggat
     1261 cgacaagcca aaatactgct tgatccagcg cttttcttcg ggcgtcggtg actccaggtc
     1321 aacccagatc ggctgaaagt gcgacaactc ttcgagcgac tcgatctctt cctgaatcag
     1381 tcgtccgttg gcgagggtaa aaatattgag catggcttac tcccaaaaca aacatcgcat
     1441 caggtggctc aaggcgatca ggcagcctgc cgtaccctgc tgcaatttgg ggccggaaaa
     1501 ctgcctgctg gggcaagttt ccaaaagggg atcgatttcg ataagtaaaa ctgcttgatt
     1561 atggcattgc ccgcgcagac caccgggcgg ggccggatga tgtccgcaaa cgctgcggta
     1621 caaaaaaaat cgttgcaata atgccatcgg cggcgcatag ttccagcggc agtcaatctt
     1681 gttttcaggt tgtcagcgcg gtgttggcaa tctttacaac caagagctct aggaggcttt
     1741 ttatgaactt gacaatcagc ggtcaccact tggaggttac ccaggctttg cgcagttatg
     1801 tcacgaccaa attggatcgg ataacgcgcc atttcgatca ggtgatggat gtcaaagtac
     1861 tgctaacgct ggagaatctc aaagaaaagg aacgcagaca gaaggcagaa tgcaatatcc
     1921 atctcaaggg tatcgagttg tttgccgaaa gcgcccatgc cgatctgtat gctgcagtgg
     1981 acgagttggt ggacaagctt gaccggcagg tggttcgtca caagggccgt ttgcagtcgc
     2041 accatcacca cgcaccgaaa cgtctgatgt aatttgaact tcacgaagcc gcccgctggt
     2101 gcgtgcggct ttttttgcgc ccgaatttca cggtgcataa ttcgcaacaa atcatgaatc
     2161 gactcgcatc catccttact gctgcgcaag tacttgtcca agtcgacgtg tcaagcaaaa
     2221 aacgggcttt tgaagaagcc ggcctgctgt ttgaaaactt gcatggtctt agccgtgccc
     2281 tggtgactga cagcctgttc tcgcgcgagc ggctcggttc caccggcctg gggcatggtg
     2341 tcgcgattcc gcatggtcgc atcaagggcc tcaaggcgcc aatggcagcg gtgttccagt
     2401 tggccaagcc gatcggcttt gaggctcctg acgacctggc tgttggcatc atgatttttt
     2461 tgctggtgcc cgaagccgcg acgcaaaagc acctggaaat tctctccgaa atcgccgaaa
     2521 tgctcagcga taccaacttg cgggaaaagc tggtgaccag tcccagtgcc gccgcgttgc
     2581 atcgcctgat ttctgactgg acttcagttc agtcgctttg atttgccgct gatcagactt
     2641 tttttgccgg agtcgtctac ttgaagccca cctttatcag cgcagatgtg ctgttcgaag
     2701 ccttccgggg cagtctgcgc tgggaatggg tggccggtct aggtgcctcc gagcggcgtt
     2761 ttgacgaggt ggccgtgcgc gctgcccgct caggtgccga tctggtgggt tacctgaact
     2821 acatccaccc ctatcgggtg cagattctgg gcgaacgcga agtggcctac ctgaccaacg
     2881 ctaccgccga agattgctcg cggcggattg cccgaatcgt caccctggag ccccccgtgt
     2941 tgattttggc tgaccggcag gttgcgccac aggcactcat gtccatgtgc gaacgcgccc
     3001 agcttcccat gttttcaaca gcgaattctt cggccttcgt gattgacgtg ctgcgtgcct
     3061 atttctccaa gcattttgct gatcgctcca ccatgcacgg cgtcttcatg gacattcttg
     3121 gtttgggcgt gatgattacc ggagagtccg gactgggcaa aagtgaactt gggctggaac
     3181 tgatttcgcg tggcaatggt ctggtggccg atgatgcggt tgatctctac cgcatcaacc
     3241 aagacaccat tgaaggccgt tgccctgagt tgctgcagaa cctgctggag gtgcgcggca
     3301 tcggactgct cgatatccgt tgcatatttg gagagaccgc cgtcaggcgc cgcaagcgtc
     3361 tgcaactgat cgtgcatctg gtgcgacgtg acaccatgga gcgcgactac gagcgtattc
     3421 cctatgaacc cctgacgcag cacgtgctcg atgtaccggt gcgcaaagtc gtgatccagg
     3481 tggttgcagg tcgtaacatt tccgtactgg tagaagctgc agtgcgcaac accatcctgc
     3541 aaattcgcgg tgtcgatacc tacaaggaat tcgtcgaacg ccaccgcaag gcaatggacc
     3601 aggccggtta acctgcacaa gcggtttgca acaccgcatt gcgcctgtca gttcggccga
     3661 actgttgaag ttatttttgc ttgacccctt aaggccaatc acggtcggtg ttggcaagtg
     3721 gcgcgggtgc aatgggcgta caggctgagc gcgtgctctg aaatcacaaa acctttttcc
     3781 agcgcaacag cgttctgccg acgttcaatt gctgcgtcat aaaactcttc gacccggcca
     3841 cagtccaggc acaccagatg gtcatggtgc tgcccttcgt tgagttcata gaccgccttg
     3901 ccgctctcga agtggcttcg cgtcaggata tcggcatgct caaactgagc cagcacgcgg
     3961 tagaccgtgg ccagaccgac atcggcatga tcttccagaa gaacccgata gacatcttcg
     4021 gccgtcatgt gccgctgagc acctttctga aagagttcca gaatcttcag ccttggcagt
     4081 gtcaccttca gtccagtgct tttcagttca tcaatcttgg tcatgcatgt tttccggtta
     4141 ccagtgttgg cgtgaaccct tcggtcacgc tgtgaaatgc tgccagtaca atgctctgat
     4201 catatcgttg ccggtttgtc ccatgtctga aactcctgtt cgttgcgtct ggttgggttc
     4261 gttgtttact gttgcgctca ccttgggtgc gtgcggcagc tttaacagcg ccagtacccg
     4321 tctggccaat gtcgtgacac cttacaagat cgatatcgtg caaggtaatt tcgtctcacg
     4381 cgaacaggcc gctgcgctca aggaaggcat gagccgcagc caggtgcgcg acattctggg
     4441 gacaccgctg ctgaccccga ttttccatgc cgaccgctgg gactacgtgt tcaccatcaa
     4501 gcgccagggt gtccaagccc aggcgcgacg cgtcacgatt tttttcaagg gtgatgccct
     4561 gttgcgcgtt gaggcagatg tgcttccgac cgaggcagaa tttgttgcat cgcttgattc
     4621 cggtcgcaag gcgggcaaag ttccgctgct cgaagcgccc gaagccagtc tccaagccac
     4681 gcccgtgccg cctgcggtgg ccgcgcccaa agcgctgcca ccgttgcgcg acagttaccc
     4741 accccttgaa gaccccgctg cccgctaagg cagtgaggga gaagatcatg tcaaaccttc
     4801 accgtatctg cgtggccggt gccactggtc gcatggggcg catgctgatc gaagccgtct
     4861 ggaatgccga cgattgccag ctcggcggcg cgctcgaact ggctggcaat gccgccatgg
     4921 ggcaagaccc ggcggccttt ttggggcgca acagcggcgt tgccatcacc gctgatatgg
     4981 ttgccgggct gcaaaattcg caggttctga ttgacttcac ccgcccgcaa gggacgctgg
     5041 cgcatttgcg ggtttgccgg caactcggtg tcaaggcggt gatcggcacc actggcttta
     5101 gcgacgctga aaaagctgaa atcgccacta tcgccaacga catcgccata gtcatggcac
     5161 ccaacatgag tgtcggtgtc aacgtcacgc tcaagctgct tgaactggca gcccaggcgc
     5221 tggccactgg ttacgacatc gagatcattg aggcgcacca ccgccacaag gtcgatgcac
     5281 cgtcgggcac ggcactgaaa atgggcgaag tgattgcctc tgccctgggg cgcaacctca
     5341 atgactgcgc ggtctatgac cgccacggca tcaccggcga acgtgacccg tccagcatcg
     5401 gttttgccac cattcggggc ggcgacattg ttggtgatca caccgtgctg tttgccggta
     5461 ccggcgagcg cattgaaatc agtcacaagt catcgagccg ggtcacctac gcgcagggca
     5521 gtctgcgcgc ggtgcggttt ttgcatcaac aggcaaccgg gctgtttgac atggccgatg
     5581 tgctccacct gaaatgagcc tggctcagtt cttctggcag ggcgacatgc tggcgcgggc
     5641 ggtggccctg ctgctgctgg ccatgtcggt tgccagttgg gtggtgattc tctggaaaac
     5701 atggctgatg cagcgcgcca gccgcgacgt ggcgcgctgc atcgccgcct tctggcaatc
     5761 ttcaagcatc gccgaagcaa cccaaaaatt gcagtctttt gaccgcgaag cgctagtgct
     5821 gcccatggtt ctagctacaa atattgaagc aggatcaagc ctcgcccaag ccagcggcaa
     5881 aacccagcaa ttgacccgcg tattgcgcaa tgccctgcac gcagccctgg gcaagctgca
     5941 acacggccag gtcttgctgg ccactgtcgg ggccaccgcc ccctttgttg gcttgctcgg
     6001 cacggtctgg ggcatctacc atgcgctgat tggcattgcc agcgccggtc aggtcaccat
     6061 tgacaagatt tccggcccgg tgggcgagtc gctcatcatg accgccgccg gtctgctggt
     6121 ggccattccg gcggt
//
LOCUS       DAOSLL010000241         6119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11628_length_6119_cov_4.223120, whole genome shotgun sequence.
ACCESSION   DAOSLL010000241 DAOSLL010000000
VERSION     DAOSLL010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6119
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11628_length_6119_cov_4.223120"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(127..1059)
                     /locus_tag="PLB25_11305"
     CDS             complement(127..1059)
                     /locus_tag="PLB25_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOE42198.1"
                     /translation="MKHLYLTPVVLALATLLAACGSSPKSTSLLEQAHSDYRIAQSNP
                     NVGRYAPLELKQAGDALAQADALATERASSEKIDQLAYLARQKIALTEEVAKQKMAET
                     DVRNAGQERNQVRLDQRTNEANAANIRAEQARQVALLAINDAAQSQADTDRARQQTQA
                     AQMDAANAQQRATQLEAQLGELSAKKTARGMVITLGDVLFGTDLSRLTSDGEASAKKL
                     AKILELNPQRTVLIEGFADSTGTAEYNQQLSVRRAMAVQTALQTLGVGRERVVIRGYG
                     ESYPVAANDSAQNRQMNRRVEIVLSDEAGRTIAR"
     gene            complement(1091..1465)
                     /locus_tag="PLB25_11310"
     CDS             complement(1091..1465)
                     /locus_tag="PLB25_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HOE42199.1"
                     /translation="MTRNSRRLMAVLVGAAATLMLGCSSLKTPATADVAVSKAAVDNA
                     AGAGAAQYAPVELMAARDKLALANKAMSVKDYKLARDMAAQAQADAKLAQSKANSAKA
                     QAAADAVQDDIRVLREELERNL"
     gene            1717..2337
                     /locus_tag="PLB25_11315"
     CDS             1717..2337
                     /locus_tag="PLB25_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42200.1"
                     /translation="MKSTATKFSALAFAVTLLAGVSATAQPMSYSDYQTGKSKIQADY
                     KVEKKGCSSLAGNAKDICIEQAKGKEDVSQAELYESYNPNPKSHYKARIARAEAIYQV
                     AKEICDDTTGNVEDVCRKEAKAALTFAEANATVEMKTQVANEAANAKSVAAQNQAIEK
                     TIEVRSDATADKRAAQLAVEKEKCDAFASTAKDDCLKSAQAQFGKL"
     gene            2367..2531
                     /locus_tag="PLB25_11320"
     CDS             2367..2531
                     /locus_tag="PLB25_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HOE42201.1"
                     /translation="MLHYAVVFFVIALIAALFGFGGIAAGAVGIAKILFFIFVVLAIV
                     TFLFGSLKGR"
     gene            2594..2974
                     /locus_tag="PLB25_11325"
     CDS             2594..2974
                     /locus_tag="PLB25_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42202.1"
                     /translation="MFLNKSSEKSSSLMDDAAHSADQAIKSTQQMANDALENLTSALQ
                     DLSHQATPAIERAGDRVSSLAHRSLDGVRETTHQLRVKVEHASDNTVNYIRHEPVKSV
                     LFAAATGAAVMALISLLSHTPRSR"
     gene            2999..3373
                     /locus_tag="PLB25_11330"
     CDS             2999..3373
                     /locus_tag="PLB25_11330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42203.1"
                     /translation="MHPLFALLVTRPQLLVEHALAYSALLNQESGLAYAAWRRQTLLW
                     LVALGCAAVALVLAGVAVMLWAVTSVAPVHAPWVLLGVPLLPLAVAVVCVWRAPGTDN
                     NFARLTRQVEDDLAMLRAATSS"
     gene            3370..3846
                     /locus_tag="PLB25_11335"
     CDS             3370..3846
                     /locus_tag="PLB25_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42204.1"
                     /translation="MKINPAVAERLTLSRARLRLAMAEMAAPPSQAPGAGAKPSGSGW
                     RARLAQGPATRLLLELGLAWWARQPLRQLLPLVAQAAQAVLGPTAQRHPVGLVLSAAA
                     VGAALVQARPWQWLSATALLVSLLRRLRPAVARPTRALSLARPDAPPAAAMRGPAR"
     gene            3861..4976
                     /locus_tag="PLB25_11340"
     CDS             3861..4976
                     /locus_tag="PLB25_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006162942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HOE42205.1"
                     /translation="MVPQPVLVTSGSHLALSVLLALVTLALGYILLPFYGAILWGVII
                     ALVFAPLNQWLLPRVRQQRTTAALLTLLIVLVIVVLPMAAVAAALASEAAVVYARLES
                     GDLNPALYFRGVFDALPSPITAVLDRFGLVSFNTLQRRLTAALTQGTQFIATQTFSVG
                     QNAFDFFASFFITLYLAFFLVRDGQTRLQPVWQTLPLAEPDKAHLLQTFVTTVRATVK
                     GNLLVAIIQGALGGLAFWALGVSGALLWAVLMAFMSLLPAVGAGLVWLPVATWLVLTG
                     SLWQGVGLGLYGVLVIGLVDNLLRPILIGRDTRLPDYIVLITTLGGMAVFGLNGFVLG
                     PVIAAMFIAFWQIHVAQHIAAANQQLAQLPESTSSAP"
     gene            complement(5026..5949)
                     /locus_tag="PLB25_11345"
     CDS             complement(5026..5949)
                     /locus_tag="PLB25_11345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3616 domain-containing protein"
                     /protein_id="HOE42206.1"
                     /translation="MTPFDTPSFWPMTGLYEPSAIQQLADGRFLVIEDERQRSFSMVT
                     IGPGTEVESTPLGPGWFQSSNPFWKLDDLEGLALDKLGYLYAITSHSRDDDGDEKKAR
                     GRLVRFRIDGDRVVAPVVVDGLKRALVATHPLLAAAAQLEDVKSEGGLNIEALEISAD
                     QQRLLLGFRSPLQGRLAIIASVENPVAMFESDASPQVSTTLQTLDLGGQGIRGMAFID
                     SLQGYLLISGPVSRAQQAFGLWFWSGQPDAPARRVSVAGLPGFERAEGLSPAVLDGKP
                     KIVIVSDDGNRKEGRFARFLVLDPGQLQIAP"
BASE COUNT         1107 a         1881 c         1894 g         1237 t
ORIGIN      
        1 ggaggcgcgc cctcgcgacg aaggctcatc gattccggct cggccggctt aggtgccaag
       61 caaaaaaact tcagcagttc ggccgcgctg gcaagcgcaa tgcgttgttg caaaccgctg
      121 gtgcagttag cgggcgatgg tccggccggc ttcgtcggac aggacgattt ccacccggcg
      181 gttcatctgc cggttttgcg cgctgtcgtt ggcggccacc ggataagact caccgtagcc
      241 acgaatcacc acccgctcgc gcccgacacc gagcgtttgc agggcagttt gcaccgccat
      301 ggcgcggcgc accgacagtt gctggttgta ctcggcggtg ccggtgctgt cggcaaagcc
      361 ttcaatcagc acggtgcgct gcggattgag ttcaagaatt ttggcgagct tttttgccga
      421 tgcctcaccg tcgctggtca ggcgcgacaa gtctgtgcca aacagcacat cgccgagcgt
      481 gatgaccatg ccacgcgcgg tctttttggc agacagttcg cccagttgcg cctcgagttg
      541 ggtggcgcgc tgctgggcat tggcggcatc catctgggcc gcctgggttt gctgcctggc
      601 gcggtcggtg tcagcctggg actgagctgc gtcattgatc gccagcaaag ccacctgtct
      661 ggcctgctcg gcgcggatat tggcggcgtt ggcttcgttg gtgcgctggt ccagtcgaac
      721 ctggttacgc tcctggccgg cgttgcgcac atcagtttcg gccattttct gcttggcgac
      781 ttcttcagtc agggcaattt tctgccgggc gaggtaggcc aactggtcga ttttttcgct
      841 gctggcacgc tcagtggcca atgcatccgc ttgtgccagg gcatcaccgg cttgctttag
      901 ttccagcggc gcgtagcgac cgacattcgg gttgctttgc gcaatgcggt agtcgctgtg
      961 ggcctgctcc agcagcgaag tgcttttggg gctggagccg caggcggcaa gcagggtggc
     1021 cagcgccaga acaacgggtg tgaggtaaag gtgtttcatg atcgaatcct gtgagtagtg
     1081 ggctggtgaa ttacagattg cgctctagct cttcgcgcag cacgcgaatg tcgtcttgca
     1141 ccgcatcggc agccgcctgg gctttggccg aattggcctt gctttgggcc aacttggcat
     1201 cggcctgcgc ctgcgcggcc atgtcacgcg ccagcttgta gtccttgact gacatcgcct
     1261 tgttggccag cgccagtttg tcgcgcgccg ccatcagctc gaccggcgca tactgcgcgg
     1321 caccggcacc agcggcgttg tcaaccgccg ccttcgacac cgccacatca gccgtggctg
     1381 gggtttttaa actgctgcag cccagcatca gggtggcggc agcgccgacc agcacggcca
     1441 tcaaccgtct tgagtttctc gtcatcatgc atctccagta agtccaccaa gcgcgggatc
     1501 acacttggtg cattgggtta taggtggccc ctcgggtaag gtctgttcgc tggcgcaccc
     1561 cagcgccagc cagcccggtc aagccggggc ggcagcggca acgcctgatt tgcaatgaat
     1621 gtgtgcgagc gaacagacca gcgggtttga tcaacttacg ctcagcgccc acaagacaac
     1681 gcacctgcgc ggctgtcgca aaaccaagga ctcatgatga aatcaaccgc taccaaattc
     1741 agtgctctgg cctttgccgt caccctgttg gcgggtgtca gtgccaccgc acaaccgatg
     1801 tcgtacagcg actatcaaac cggcaagtcg aagatccagg ccgattacaa ggtcgagaaa
     1861 aagggctgct cttcgctggc aggtaatgcc aaagacattt gcattgaaca agccaagggc
     1921 aaagaagacg tgtcacaggc agagttgtac gagagctaca acccgaaccc caaatcacac
     1981 tacaaggcaa gaatcgccag ggctgaggcc atttaccagg ttgccaagga gatatgcgac
     2041 gacaccacag gcaacgttga ggacgtttgc cgcaaagaag ccaaggccgc cctgacattc
     2101 gccgaggcca atgccaccgt cgagatgaaa acccaggttg ccaatgaggc cgccaacgcc
     2161 aagtcagtcg cagcccagaa ccaggctatt gaaaaaacca tagaagtccg cagcgatgca
     2221 acagcagaca agcgggcggc gcaattggcg gttgaaaaag aaaagtgcga tgccttcgcc
     2281 tcaaccgcca aagacgactg cctgaaaagt gcgcaagccc aatttggcaa gttgtaggtg
     2341 tttttcttca acccaggagt ctcaccatgc tgcattacgc cgttgtgttt tttgtgattg
     2401 ccctgattgc ggccttgttc ggctttggcg gcattgccgc tggggcggtt ggcattgcca
     2461 aaatcctgtt tttcatcttc gtcgttttgg caatcgtcac ctttctcttc ggctcgctca
     2521 agggacgcta gcagaggggc gcgaatgcgc cagcgatgcc gctttgattc attcactcac
     2581 tcaaggaaaa atcatgtttc tcaacaaatc atctgaaaag tccagcagcc tgatggacga
     2641 cgccgcccat tcggccgacc aggccatcaa atcgacccaa caaatggcca acgatgccct
     2701 ggagaatctg acatcggcct tgcaagacct gagccaccag gccacccccg ccattgagcg
     2761 tgccggtgac cgggtcagtt cactggcgca ccgcagcctc gacggtgtgc gcgagaccac
     2821 ccatcaactg cgcgtgaaag ttgaacacgc ctcagacaac accgtgaact acatacggca
     2881 cgagccggtc aaatcggtgc tgttcgcagc ggccaccggt gctgccgtga tggcgctgat
     2941 cagcctgttg agccacaccc cccgcagccg ctgatcaggc tggcacgcgc ggcaccagat
     3001 gcatccccta tttgccttgc tggtgacccg gccgcaactg ctggtggagc acgctctggc
     3061 ttactcggcg ttgctcaacc aggagtctgg cctggcctat gccgcgtggc ggcgccaaac
     3121 cctgctttgg ctggtggccc tgggctgtgc cgctgtggca ttggtgctgg caggtgtggc
     3181 ggtcatgcta tgggcggtga catcagttgc gccggtgcac gcaccctggg tcttgttggg
     3241 cgtgccactg ttgccgctcg ccgtggcagt ggtctgcgtg tggcgggcac caggcactga
     3301 caacaatttt gccaggctga cacggcaagt tgaagacgac ctggcgatgc tgcgcgccgc
     3361 cacttcatca tgaagatcaa ccctgccgtc gctgaacggc tgacgctgtc ccgcgcgcgc
     3421 ctgcggctgg ccatggccga aatggccgca ccaccaagtc aggcaccggg agcaggtgcc
     3481 aagccgtctg gctcgggctg gcgcgcaagg ctggcgcaag gccccgccac ccgcctgctg
     3541 ctggagctgg gcttggcctg gtgggcgcgg caaccgctgc ggcagttgtt gcccttggtt
     3601 gcgcaggccg cccaggcggt gctgggacca accgcacaac gccatccggt cgggctggta
     3661 ctgagcgcgg cggcggtcgg cgcggcactg gtacaggcgc gcccctggca gtggctttcg
     3721 gcaaccgcct tgctggtcag cttgttgcgc cgcttacgcc cggctgtcgc caggcccacc
     3781 agggcacttt ccctggcgcg gccggacgca ccaccggctg cggcgatgcg cggtccagcg
     3841 cgttgagcaa aatgcccgtc atggtcccac agcccgttct ggttacctct ggcagccatc
     3901 tggcgctttc agtgctgctg gcgctggtga cgctggcact gggctatatc ttgctgcctt
     3961 tttatggggc cattctgtgg ggggtgatca ttgcgctggt gtttgcgccg ctgaaccaat
     4021 ggctgctgcc ccgggtgcgc cagcagcgca ccactgcggc actgctgacg ctgctgatcg
     4081 tgctggtcat tgtggtgctg ccaatggcag cggtggcggc cgcactggcc agtgaagcgg
     4141 ctgtggttta cgcccggctg gaaagcggcg acctcaatcc ggcactctat tttcgtgggg
     4201 tttttgatgc gctgccgagc ccgatcacag cggtgctgga ccgcttcggg ctggtcagtt
     4261 tcaacacctt gcagcgccgc ctgaccgctg cgctgaccca gggcacccag ttcattgcaa
     4321 cccagacctt cagcgtcggg caaaacgcgt ttgacttctt cgccagcttt ttcatcaccc
     4381 tgtacctggc gtttttcctg gtacgcgacg gccagaccag gctgcagccg gtctggcaga
     4441 cgttgccact ggcggaacct gacaaggcgc acttgctgca gacctttgtg acgactgtgc
     4501 gggccaccgt caagggcaat ttgctggtgg cgatcattca gggcgcgttg ggcgggctgg
     4561 cgttctgggc gctgggggtg agcggtgcct tgctgtgggc ggtgctgatg gccttcatgt
     4621 cgctactgcc cgcagtgggc gcgggcctgg tgtggctgcc ggtggcgacc tggctggtgc
     4681 tgaccggctc gctctggcaa ggggtcgggc tggggcttta cggggtgctg gtgatcgggc
     4741 tggtggacaa cctgttacga cccatcctga tcgggcgcga cacccgcttg ccagactaca
     4801 tcgtgctgat caccacgctg ggcggcatgg cggtatttgg cttgaatggt tttgtgctgg
     4861 ggccggtgat tgcggccatg ttcattgcct tttggcagat ccatgtggcc cagcacattg
     4921 ctgcggccaa ccagcaactg gcgcagttgc ccgagtcaac cagcagcgcc ccgtaacaag
     4981 acaaaaaaac ttcagcgaaa gtgacccggc caccgggcat gacaatcaag gtgcaatctg
     5041 caactgccct gggtccaaca ccagaaaccg ggcgaatcga ccttcttttc ggttgccgtc
     5101 gtcgctcaca atgacgattt ttggcttccc atccagcacc gccgggctca gtccttcagc
     5161 gcgctcaaat cctggcaagc cagcaaccga cacgcgccgt gccggcgcat cgggctgacc
     5221 gctccagaac cacagcccga acgcctgctg cgcgcgcgat accgggccac tgatcagcag
     5281 gtaaccctgc agcgaatcaa tgaaggccat gccgcgaatg ccctgtccgc ccaggtccag
     5341 cgtctgcagc gtggttgaca cctgtggcga agcgtcagat tcaaacatcg ccacaggatt
     5401 ctcgacgctg gcgatgatgg caaggcggcc ctgcaacggg cttcgaaaac cgagcaacag
     5461 ccgttgctgg tccgcgctga tctcaagtgc ttcaatattg agcccaccct cggacttgac
     5521 gtcttccaac tgggcagcgg cagctagcag tgggtgggtt gcgaccagcg cccgcttgag
     5581 cccatccacc accaccgggg ccactacccg atcaccgtcg atgcgaaatc gcaccaatct
     5641 tccccgtgcc tttttttcgt cgccatcatc atctcgcgag tgcgatgtga ttgcgtaaag
     5701 gtagccgagc ttatccaggg ccagcccttc aaggtcgtcg agcttccaga acgggttgct
     5761 gctttgaaac cagccggggc caagcggggt gctttcgacc tcggtgccgg gcccgatggt
     5821 gaccatgctg aacgagcgct gccgttcatc ttcaatcacc aggaaacggc cgtcggcaag
     5881 ctgctggatg gcagacggtt cgtacaggcc cgtcatgggc caaaagctgg gggtgtcaaa
     5941 aggtgtcatg gcaaacattg tgactcaagg gctgatggtc atctcaagcg atgggccggc
     6001 atttggcctt ggcgcgacag atttgcccct tcagaaggcg cgtcgattca acctagattc
     6061 ctcggttgga cgcgcccgag tgggccgctg gcggcgcgtc tgggtaggcg cgacgacga
//
LOCUS       DAOSLL010000242         6110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11648_length_6110_cov_3.991412, whole genome shotgun sequence.
ACCESSION   DAOSLL010000242 DAOSLL010000000
VERSION     DAOSLL010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11648_length_6110_cov_3.991412"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..344)
                     /gene="fliE"
                     /locus_tag="PLB25_11350"
     CDS             complement(27..344)
                     /gene="fliE"
                     /locus_tag="PLB25_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HOE42207.1"
                     /translation="MNLRLTPTTLPVAVRPNLSAGRAAGQVSGADAGGFSGALQSALS
                     SVSAAQNDAAKLQREVQLENPKVSLEETMVAIQKAQIGFQATLHVRNRMVQAYTDIMN
                     MQV"
     gene            complement(508..1155)
                     /locus_tag="PLB25_11355"
     CDS             complement(508..1155)
                     /locus_tag="PLB25_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013232762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HOE42208.1"
                     /translation="MTSLDNTTTIAEPYKSRLDALLSSGRRKILGIVGPPGCGKSTLA
                     RSLLALLEKSAVAVPMDGFHLANVELARLGRAGRKGAEDTFDSAGYVSLLHRLKTQQP
                     NEIVYAPEFRREIEEPIANAIPVFPETPLIITEGNYLLLDHGHWAAVRALLDEVWYVD
                     IDQCVRRERLVKRHMQFGRSPTAAEDWVANTDDPNAVQIEATKSRADILFKWKQD"
     gene            complement(1360..1435)
                     /locus_tag="PLB25_11360"
     tRNA            complement(1360..1435)
                     /locus_tag="PLB25_11360"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1400..1402),aa:Lys,seq:ttt)
     gene            1636..1833
                     /locus_tag="PLB25_11365"
     CDS             1636..1833
                     /locus_tag="PLB25_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42209.1"
                     /translation="MRLTAILPVLPGAQVVNGKQIIFAITDDDKDTDGSIRLPGFSSR
                     PGWPWHGAAAGQTTKLLTLPE"
     gene            complement(1953..2813)
                     /gene="hpnC"
                     /locus_tag="PLB25_11370"
     CDS             complement(1953..2813)
                     /gene="hpnC"
                     /locus_tag="PLB25_11370"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HOE42210.1"
                     /translation="MSPGVGLAAVLPVHHYENFPVASLLCPAHLRPAVAAIYHFARTA
                     DDLADEGAQDAATRLTDLQAFRADLQAVASGQVASERWLAVFEPLRSALLMHDLPLNL
                     LTDLLEAFEQDVRYTAAQRRYATDAELLDYCQRSANPVGRLLLHLYGVTDEEALTQSD
                     QICTALQLINFWQDVSLDVARGRWYPSIEAMARCQVQDADLRSGSHSKNATVLIAGYA
                     AAASAGMLKGSKLAVTLPGRIGWELRLVVQGGLRILDKIEALQFATWRTRPKLTFWDL
                     PVMLWRALWM"
     gene            2951..4171
                     /locus_tag="PLB25_11375"
     CDS             2951..4171
                     /locus_tag="PLB25_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOE42211.1"
                     /translation="MNLLNVRTRVIPLGLTIAAALGLVACSKPPAPQEPIRAVKVITV
                     GVAPLQAGLEYAAEVRARVESRLGFRVGGKLIARPAEVGQRVKVGQLLAQLDPQDLKL
                     ATDAARAQVAAAQTNRDLAVADFKRFQSLKAQNFISGADLERRDSTQKAAQAQLELAQ
                     SQLAVQGNQSAYANLLADVAGVVTAVLAERGQVVAAGAPVVQVAQDGVRDAVFAVPED
                     KVAAIKPGAGVQVRVWSGNTTLQGLVREVAASADPVTRTFSVKVALPAGSDLPLGSTV
                     SVLPAALQHSGLQAIKLPTSALRQEGQGSAVWVLDVPSMTVKLQPVQIATADGNDVVV
                     AAGLSPGMQVVVAGVHVLTVGQKVTVYKEKQAIALSSKAQTAPENVANDGLRQALAAG
                     GAASALGAASAVAN"
     gene            4181..>6110
                     /locus_tag="PLB25_11380"
     CDS             4181..>6110
                     /locus_tag="PLB25_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOE42212.1"
                     /translation="MSETHLHPERSFNLSRWALEHAPLTRFLMIVLMVLGASAYFQLG
                     QDEDPPFTFRAMVVRAYWPGASTQQMAEQVTDKLERTLQEVPYADKIRSYSKPGESQI
                     IFQIKEIARGAEVANVWYQVRKRIGDMRGTLPPGVQGPFFNDDFGDVFGVIYALQAPG
                     FSYAEVKTFADDVRQQLLRVPDVAKVELFGVQDEKLFVEISQKRLAQLGLDINAVLAQ
                     LTQQNAVESAGAVQTPLDVVQVRVAGQFQAVEDLRAMPIRGTGYAAGSNAGGSQLRLG
                     DIAEIKRGYQDPPSIKVHFQGQEVVALGVSMTKGGDIIALGKALKEATAQLEKTLPVG
                     VTLAQIQDQPKSVATSVNEFVKVLIEAVAIVLAVSFISLGLHKRPGQHPFWQRWTLDV
                     RPGLVVGITIPLVLAMTFLAMNYWGIGLHKISLGSLIIALGLLVDDAIIAVEMMVRKM
                     EEGYDKVRAATFAYEITAMPMLTGTLITAAGFLPIGLARSVTGEYTFAIFAVTVIALC
                     LSWIVSVYFVPYLGVRLLKVPPHVALAAGQDGAPVGVAGHDMYDTAFYTWFRGLVNAC
                     VRHRWITISVTLAMFGAGLLGMGQVQQQFFPDSSRPELTVDLWSPEGTSFAATEEVAK
                     RVEARLLAEPGVTSVAQWV"
BASE COUNT         1184 a         1685 c         1933 g         1308 t
ORIGIN      
        1 aataagggct acaagcctaa aacaccttaa acctgcatat tcatgatgtc ggtgtaagcc
       61 tgcaccatcc ggttcctgac atgcagtgtc gcctgaaagc cgatttgcgc tttctgaatg
      121 gccaccatgg tttcttccag gctgaccttt ggattttcca actgcacttc gcgctgcagt
      181 ttggctgcat cgttttgcgc tgcgctgacc gaactgagcg cgctttgcag cgcacctgaa
      241 aatccgcctg catccgctcc actcacttgc ccagcggccc gtcctgcaga caggttgggg
      301 cgaaccgcaa ctggcagcgt ggtaggagtg agtctgaggt tcatggcgtt agtggaccga
      361 gataaaggta tggcatggat actagcaacg ccaactcggg gccatgaatt tgaagtagcc
      421 ggtaaaaacc ggtcttatcg agcttttgtt ttcaaatcag agcgaaacat tcgggctcca
      481 tgccgggcag ggttgttgtc gccgtgatca atcctgtttc catttgaaca ggatgtcggc
      541 ccgcgacttg gtggcctcga tctgcacggc attcgggtcg tcggtgtttg caacccagtc
      601 ttctgccgca gtcggtgacc gcccaaactg catgtggcgc ttgaccaggc gttcccgtcg
      661 aacgcactga tcaatatcca cataccagac ttcatcaagc agggctctga cggcggccca
      721 gtgaccgtga tcgagcaaca gatagttgcc ttcggtgatg atcagcggtg tttctggaaa
      781 gaccggtatc gcgttggcga tcggctcttc aatttcgcgc cggaactccg gtgcatacac
      841 tatctcattg ggttgttgcg ttttgaggcg atgcaacagc gatacgtacc cggcgctgtc
      901 gaaagtatct tcagcgcctt tgcgcccggc tcgaccaaga cgggccagtt cgacatttgc
      961 caggtgaaag ccatccatcg gaacggccac ggcagacttt tcaagcaacg ccaggagcga
     1021 ccgcgcaagc gtcgatttgc cgcatccggg tggcccaacg ataccgagaa tctttcgcct
     1081 gccgctgctt aacaaggcat caagcctcga tttgtagggt tcggcaatcg ttgttgtgtt
     1141 gtctagggaa gtcattcttg aggtcctata aatgaactga actcaatcaa gccattttga
     1201 tccccttgcc aaggcaaatg catgcggtgt gcaccaatgc tgcagcagcc tgaactgccg
     1261 agcaagcagc aaacggcaac ggtaagccag aatggcggtc aaaaataaag cccgctactg
     1321 ttgaaatagc gagcctctca atgcctgcat ggcctaattt ggtgggatgt gcgggggtcg
     1381 aacccacgac aaacggatta aaagtccgct gctctaccaa ctgagctaac atcccaatcg
     1441 cccgtcagac tagactttgg accagcgcga ttatagacag tttcctgttc attttttggc
     1501 cagagcctgg gatgcctggt gggtttcgcg tcacgctatt gatcttcttc agattgatga
     1561 cttgttgcaa agtagccttc agccagcaag tggcgatgtt tgcggagtgt ctttgagtgg
     1621 acttgcgctt ggagcatgcg gctgactgca attttgcccg tgctgccggg tgcccaggtg
     1681 gtcaacggca aacagatcat cttcgccatt accgatgacg acaaagacac cgatggcagc
     1741 atccggttgc caggattcag cagccgaccc ggctggccct ggcatggtgc tgcggcaggt
     1801 caaactacca agctgcttac attgcctgag tgaggcctga ttttgctgtt ggggcaactg
     1861 gcacttgttg gcctggcgcg ggcggcggtg atgggcaggg ggctcgcgcg ttggcgcatg
     1921 agggtggggc gatgttgtcg cggcgagcag actcacatcc acaatgcccg ccacagcatc
     1981 acgggcaggt cccaaaatgt cagcttgggt cgggtgcgcc aggtggcaaa ttgcagcgcc
     2041 tcgattttgt ccagaatgcg caaaccgcct tgcaccacca accgcagttc ccatccaatg
     2101 cgccctggca gtgtcactgc caatttggag ccttttagca tcccagcgct tgctgctgct
     2161 gcgtagccag ctatcaaaac agttgcattt ttggaatggc taccagaccg taggtcagcg
     2221 tcctgcacct ggcagcgggc catcgcttcg atgctggggt accagcggcc gcgcgccaca
     2281 tccaggctca cgtcctgcca gaagttgatg agctgcagcg cggtgcaaat ctgatcactt
     2341 tgcgtcagcg cttcttcatc tgttacgcca tacaggtgca gcagcagtcg gcccaccggg
     2401 ttggccgagc gctggcaata atccagtaac tcggcatcgg tcgcgtaacg gcgttgcgcg
     2461 gcggtgtacc gcacatcctg ctcaaaagct tcgagcagat cggtcagcag gttcaagggc
     2521 aggtcatgca tcagcagcgc gctgcgcagc ggctcgaaca ccgccagcca gcgctcgctg
     2581 gcgacttgcc cactggccac tgcctgcaag tcggcccgaa aggcctgcag gtcggtcagg
     2641 cgggtggctg catcctgtgc gccttcgtcg gccaggtcgt ccgcagtgcg ggcaaagtgg
     2701 taaatggcag caaccgccgg gcgcaaatgt gccgggcata gcagggaggc caccggaaag
     2761 ttttcgtaat ggtgcaccgg cagcaccgca gccagcccaa cgccaggcga catcgcgggg
     2821 cggtggttga caatgacgct aggtggtttg ggcatgaaag gcaatggcag attgggtttg
     2881 cggcgtgcac aaattaccta aaattggtag ccaaccggtc ctcgtcattt gtctgtcaga
     2941 aagttttgcc atgaatttgt tgaatgtgcg cactcgggtg ataccgcttg ggttgactat
     3001 agccgctgct ttagggctgg tggcctgctc caagccgccg gcaccgcagg agccgattcg
     3061 cgccgtcaag gtcatcacgg taggtgttgc gccgcttcag gccgggctgg agtatgccgc
     3121 tgaagtgcgc gccagggttg agtcgcggct gggttttcgg gtcggtggca agctgatagc
     3181 ccgcccggcc gaggtgggtc agcgggtcaa ggtggggcag ttgctggcgc agcttgaccc
     3241 gcaagacctg aagctggcga ctgatgcagc ccgcgcccag gtggctgcag cgcagaccaa
     3301 tcgtgatctg gcagtggcgg atttcaagcg ttttcagtcg ctcaaggcgc aaaacttcat
     3361 cagcggtgcc gatcttgagc ggcgtgacag cacccagaag gcagcgcagg cgcaacttga
     3421 actggcgcag tcacagttgg cggtgcaggg caaccagagc gcttatgcca atctgctggc
     3481 tgacgtggcg ggcgtggtca cagcggtgct ggccgaacgc ggccaggtgg tggctgctgg
     3541 cgcaccggta gtgcaagtcg cgcaggacgg tgtacgtgat gcggtgtttg cggtgcccga
     3601 ggacaaggtg gccgccatca agccgggtgc gggcgtgcag gtgcgggtct ggtcaggcaa
     3661 caccacgctg caaggcctgg tgcgtgaagt ggccgccagt gccgacccgg tgacgcgaac
     3721 ctttagcgtc aaggtggcat tgcccgcagg cagcgacctg ccgctcggca gcacggtgtc
     3781 ggtgctgccg gcggcgctgc agcacagtgg gctgcaggca atcaaactgc cgaccagcgc
     3841 gttgcgccaa gagggccagg gcagcgctgt ctgggtgctt gatgtgccca gcatgaccgt
     3901 taagctgcaa ccggttcaaa ttgccaccgc agatggcaat gatgttgtag tggcagcggg
     3961 cctgagccct ggcatgcagg tggtggtagc gggggtgcat gtgttgacag tcgggcaaaa
     4021 agtcactgtt tacaaggaaa aacaggctat agccctttcc agcaaagcgc agacagctcc
     4081 tgaaaatgta gctaatgacg gtttgcgtca ggccttggct gcaggcggcg cggcaagcgc
     4141 attaggcgcg gcctctgctg tggccaattg aggaccagcc atgtctgaga cacatttgca
     4201 tcctgaaaga tcttttaacc tgtcgcgatg ggcgcttgag catgcgcctc tgacacggtt
     4261 cttgatgatt gtgctgatgg tgctgggggc ttcggcctac tttcagcttg gccaggatga
     4321 agacccgcca tttaccttcc gggccatggt ggtgcgcgca tactggccag gggccagcac
     4381 ccagcagatg gccgagcagg tcaccgacaa gctcgaacgc acgctgcaag aggtgcccta
     4441 tgccgacaaa atccgcagtt attccaagcc gggtgagtcg cagatcatct tccagatcaa
     4501 ggaaattgcc cgtggggctg aggttgccaa cgtctggtac caggtgcgca aacgcattgg
     4561 tgacatgcgt ggcacactgc ccccgggggt gcaggggccg tttttcaacg atgactttgg
     4621 tgatgtgttt ggtgtgattt acgcgctgca agcgccaggc ttcagttatg ccgaggttaa
     4681 aacctttgct gacgatgtgc gccagcaact gctgcgtgtg cctgatgtgg ccaaggtgga
     4741 actgtttggc gtgcaggacg aaaagctgtt tgtcgaaatt tcccaaaagc gcctggcgca
     4801 gctcggcctc gacatcaacg ccgtgctggc gcaactgacc cagcaaaacg cggtggagtc
     4861 ggccggtgcc gtgcaaaccc cgctggatgt ggtgcaggtg cgggtcgccg gtcagtttca
     4921 ggcggtcgaa gacttgcgcg ccatgccgat acgtggcacc ggttacgccg caggcagcaa
     4981 cgccgggggc agccagttgc ggttgggcga tatcgccgag atcaagcgcg gctatcagga
     5041 cccgccgtcg atcaaggtgc attttcaggg ccaggaggtg gtcgctctgg gcgtgtcgat
     5101 gaccaagggc ggcgacatca tcgcgctggg caaggccctc aaggaagcga ccgcacagct
     5161 tgaaaagacc ttgccggtgg gtgtcacact ggcgcaaatt caggaccagc ccaaatcggt
     5221 ggccacttcg gtcaacgaat ttgtcaaggt gctgattgaa gcggtggcga ttgtgctggc
     5281 ggtgagcttc atcagcctgg gcctgcacaa gcgccccggc cagcacccgt tctggcagcg
     5341 ctggacgctt gacgtgcgcc cggggctggt ggtgggcatc accattccgt tggtgctggc
     5401 aatgactttt ctggccatga attactgggg catcgggctg cacaagatat cgctgggttc
     5461 gctcatcatc gccttgggct tgctggtgga cgatgccatc attgccgtcg agatgatggt
     5521 gcgcaaaatg gaagagggtt atgacaaagt tcgcgccgcc acctttgcct atgagatcac
     5581 cgccatgccg atgctaaccg gcacgctgat cacggcggct gggtttttgc cgatcgggct
     5641 ggcgcgctca gtcaccggtg agtacacctt tgcgattttt gcggtgacgg tgatcgcttt
     5701 gtgcctgagc tggatcgtct cggtttattt tgtgccttat ttgggcgtgc gcctgctcaa
     5761 ggtgccgcca catgtggcat tggctgcagg tcaggacggc gcgcccgttg gcgttgccgg
     5821 gcacgacatg tacgacacgg cgttttacac ctggtttcgt ggcttggtga atgcctgtgt
     5881 gcggcaccgc tggatcacca tttcggttac gctggcaatg tttggcgcgg ggctgctcgg
     5941 catggggcag gtgcagcagc agttttttcc ggattcaagc cgtcctgaac ttaccgtcga
     6001 tttgtggtca cccgagggca cctcttttgc ggccaccgaa gaggtggcca aacgggtcga
     6061 agcacgtttg ctggccgagc ctggcgtgac ttcggtagcg cagtgggtgg
//
LOCUS       DAOSLL010000243         6088 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11702_length_6088_cov_4.460467, whole genome shotgun sequence.
ACCESSION   DAOSLL010000243 DAOSLL010000000
VERSION     DAOSLL010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11702_length_6088_cov_4.460467"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            409..795
                     /locus_tag="PLB25_11385"
     CDS             409..795
                     /locus_tag="PLB25_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOE42213.1"
                     /translation="MKSNPVGWFEIYVQDMPRAKAFYEAVFQGTLEELKNPDPKGFPD
                     MEMWAFPMSVDGFGAAGALVKMAGFASGGSTLVYFSCEDCSVEAARAASHGGRVFKEK
                     MSIGEYGFIALFGDTEGNMIGLHSLQ"
     gene            complement(1048..1530)
                     /gene="ispF"
                     /locus_tag="PLB25_11390"
     CDS             complement(1048..1530)
                     /gene="ispF"
                     /locus_tag="PLB25_11390"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HOE42214.1"
                     /translation="MTFRVGEGWDIHALVPGRKLMLGGIEVPFHFGLLGHSDADALLH
                     AITDALLGAAALGDIGTHFPDTDARFQGVDSSVLLFDTVKRVRSYGFEIGNIDSTVIA
                     QAPKLMPYIAAMRAKIAQTLGIGLDQINIKAKTFEKMGPVGQGLAIEARALVLLFKKI
                     "
     gene            complement(1527..2285)
                     /gene="ispD"
                     /locus_tag="PLB25_11395"
     CDS             complement(1527..2285)
                     /gene="ispD"
                     /locus_tag="PLB25_11395"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HOE42215.1"
                     /translation="MTDSNPSHPKASADRLVNPVAVRLHALIPSAGTGTRSGAAGPKQ
                     YQALAGKPLVLHTLAAFAGVSRIDLTLVVVASDDTHLAAIRPQDAAYSIATCGGSTRA
                     ASVFNGLNWLIQEGAAAHDWVLVHDAARCLIRPDQINRLIDACWLDEVGGLLALPLPD
                     TLKQERHGRVAATLDRHDKWLAQTPQMFRIGSLLDALQVAGDNVTDESSAIEALGLQP
                     KLVSGSAQNFKVTYPEDFSLADAVLQSRHTRTAT"
     gene            2379..5942
                     /gene="mfd"
                     /locus_tag="PLB25_11400"
     CDS             2379..5942
                     /gene="mfd"
                     /locus_tag="PLB25_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HOE42216.1"
                     /translation="MDLPKLTPGKRYTLPRPTGSADALLLAQLALREKAQGKVTAIVT
                     AAAPDAQRLIDEMAFFAPKLRCVLFPDWETLPFDTFSPHQDLISERLATLWRISQHDR
                     DTGADVVLVPATTALYRLAPPSFLAGTTFHFKVKQKLNESRLKAQLTLAGYSHVTQVV
                     SPGEYAVRGGLIDLFPMGSPVPYRVDLFDDEIDSIRTFDPDSQRSLYPVPEVRLLPGR
                     EFPMDDAARTRFRHRWRELLDGDPTRSRIYKDIGLGVATAGIEYYLPLFFESTASVFD
                     YLGDAATVVLHGDLDQAFAHFWQDTRERYRLVQGDPERPVLPPDALFLNAEQFYNRVN
                     QHAQLALRAARVAPFQQESAPSFPQDSSPSIDLASGHDGGLAQAYAEFDALPPLTVVR
                     GAEDPLVSLKRHISHTAQRVLVLAESDGRRASLLDFLHSSAFDPPTFDSLAGFLASAE
                     KVGIATSNLATGFSWLESGVDFVTETELFAASPTSRRRKKQEQVSDIEALIKDLSELK
                     VGDPVVHSAHGIGRYRGLINLDLGQKLANGEPEIQEFLHLEYADKATLYVPVSQLQLI
                     SRYTGVSPDEAPLHKLGSGQWDKAKRKAAEQVRDSAAELLNIYARRAAREGHAFRYSP
                     NDYEAFANDFGFEETADQCAAIHAVIQDMISPRPMDRLICGDVGFGKTEVALRAAFIA
                     ITGGKQVAFLAPTTLLAEQHFQTLVDRFAKWPVKVAEMSRFRSGKEITAALKGVADGT
                     IDIVVGTHKLLSQSTKFKDLGLLIIDEEHRFGVRHKEQMKALRAEVDVLTLTATPIPR
                     TLGMALEGLRDLSVIATAPQRRLAIKTFVRTEGNGVIREAVLRELKRGGQVYFLHNEV
                     ETIENRRAKLEELLPEARIAVAHGQMPERQLEAVMRDFVAQRSNLLLCSTIIETGIDV
                     PTANTIVMSRADKFGLAQLHQLRGRVGRSHHQAYAYLMVPDMEGLTKQATMRLDAIQA
                     MEELGSGFYLAMHDLEIRGAGEVLGENQSGNMLEVGFQLYNEMLSEAVRCLKAGIEPD
                     LLSPLNVATDINLHAPALLPDDFCGDVHLRLSFYKKLATAKTTNQIDALLEEIIDRFG
                     KLPAQAQTLIDVHRLRVLSQPYGVTKVDAAPGVITITFRKDPPIDAMKIIALIQKNKH
                     IRLAGNEKLRIERDLPEAKDRAQMVREVLRNLGQPVPQLAS"
BASE COUNT         1269 a         1830 c         1770 g         1219 t
ORIGIN      
        1 acaagcggtt tgcaacaacg cattgcgctt gtcagtgcgg ccgaactgtt caagtcattt
       61 ttgcttgacg ccttagctca gttgccaggc cagagacgca ccgtacaccg gttttgacat
      121 tgcacttgca tgtcgccgtg gtcaccgcaa aagccaagct tgctgcgccg caagctaacg
      181 agaaccaaat agccggtacc gcacagcatc agcgtgatgg cgtgcactct gatggcgtag
      241 ctttttgtct gcgttggccg cttgcggcat gtcgtattgc aaccgggaac gtcatgatga
      301 tagtcatcac ctcacgccag cgagaccggc cacaagctgc tcttttgtac tgcccgtgag
      361 ctttaacctt aggcctcatc acaagtcgtt cactatagga gaaacaccat gaaaagcaat
      421 ccggttggct ggtttgaaat ctatgttcag gacatgcccc gcgcaaaagc gttctacgaa
      481 gcggttttcc agggcacatt ggaagagttg aaaaatcctg atccaaaggg ctttcctgat
      541 atggaaatgt gggcattccc gatgtcagtg gacgggtttg gggctgcggg cgcactggta
      601 aaaatggctg ggttcgcctc tggcggcagt accttggtct atttttcctg tgaggactgc
      661 tccgttgagg cggcgcgagc cgcttcgcac ggcggaagag ttttcaagga aaaaatgtcg
      721 attggtgaat atggctttat tgcgttgttt ggggacaccg aaggcaacat gattggcctg
      781 cattcattgc aataaaagcc tggctggctt gcctcacctg cggccaggtg ccccagcgca
      841 ccgccccatg gtcggccacc acgcccgctg gttagccaaa cgaaaacctt tgggtcattg
      901 ccgcgttgat ggcaaatcgc ccggttgccg cattgatggc atttcgaaca actggtattg
      961 atccagaaca gtgccgtgca accatgccat ttgctagcta ttttatagct tgtcgcgcat
     1021 gtcccataag cgctagaggc atatttctca tatctttttg aaaagcagca caagcgcccg
     1081 ggcctcaatc gccaggccct gcccgaccgg ccccatcttt tcaaaagtct tggctttgat
     1141 gttgatctga tcgagcccga tgccaagcgt ttgcgcaatc ttggcgcgca tggccgcaat
     1201 gtagggcatc agctttggtg cctgggcaat gacggtgctg tcgatgttgc caatctcgaa
     1261 gccgtacgaa cgtactcgtt tgacggtgtc aaacaggagc acgctggaat ccacaccctg
     1321 aaagcgtgca tcggtgtcgg gaaaatgcgt gccaatgtcg cccagcgccg ccgcacccaa
     1381 cagcgcatcg gtgatggcgt gcagcagcgc atcggcatcc gagtggccca gcaagccgaa
     1441 gtgaaacggc acctcaatgc cgccgagcat cagcttgcgt ccgggcacca gtgcgtgaat
     1501 gtcccagccc tcccccactc tgaatgtcat gttgctgtcc ttgtgtggcg actttgcagc
     1561 accgcatcgg ccaggctgaa gtcttccgga taggttacct tgaagttttg tgcgctgccg
     1621 ctcaccagct ttggttgcag gccgagcgct tcaatggcgc tagattcgtc cgtcacgttg
     1681 tcaccggcta cctgcaaggc atcaagcagg ctgccaatgc gaaacatctg cggggtctgc
     1741 gccagccatt tgtcgtgtcg atcaagcgtt gccgcaaccc ggccatggcg ctcttgtttc
     1801 aaggtgtcgg gcagcggcag cgccagcagt ccacccactt cgtccagcca gcaggcatca
     1861 atcagtcggt tgatctggtc agggcgaatc aggcaacgcg ctgcgtcatg caccagcacc
     1921 cagtcatgcg cggcagcgcc ttcctgaatc agccaattca gaccattgaa tacgcttgca
     1981 gcccttgtag aacctccaca agtagctatt gaatatgcag catcttgggg gcgtatggca
     2041 gccagatggg tatcgtctga tgccaccacc accagcgtca ggtcgatgcg cgatacgcca
     2101 gcaaaagccg ccagcgtgtg cagcaccagc ggcttgccag ccagcgcctg gtactgcttg
     2161 ggccccgctg cgccagagcg ggtgccggtg ccagcgctgg ggatcagcgc atggagtcgc
     2221 accgcaaccg gattgaccag acgatcagcc gacgcttttg ggtgagatgg gtttgaatcg
     2281 gtcatgtgcg ccgcattcta gaatccccca gtgctttcct tctcacaccc tgccaaagct
     2341 tgcgcggggt gtcggcgttt ctatctggca aacaagacat ggatttaccc aaactcaccc
     2401 ccggcaaacg ctacaccctg ccccgcccga ccggctcggc tgacgcgctg ctgctggccc
     2461 aactggcgct gcgcgaaaag gcgcaaggca aggtcaccgc catcgtgact gcagccgcac
     2521 ccgatgccca gcgcctgatt gacgagatgg ctttttttgc ccccaagctg cgctgtgtgt
     2581 tgtttccgga ctgggaaacc ctgccgtttg acaccttttc accgcaccag gacctgatca
     2641 gcgagcgcct ggccaccctg tggcgcatca gccagcatga ccgtgacacc ggcgctgacg
     2701 tggtgctggt gcccgccacc accgcgctgt accggctggc accgccgagc tttctggcgg
     2761 gcaccacctt tcatttcaaa gtcaaacaaa agctcaacga gtccaggctc aaggcgcagc
     2821 tcaccctggc cggttacagc catgtgacgc aggtggtaag ccctggtgaa tacgccgtgc
     2881 ggggtgggct gattgacctg tttccaatgg gttcaccagt gccgtatcgg gttgacttgt
     2941 ttgacgacga aattgacagc atccgcacct tcgacccgga cagccagcgc agcctgtacc
     3001 cggtgcccga agtacgcctg ctgcccgggc gcgaatttcc gatggacgac gcagcgcgga
     3061 cgcgctttcg gcaccgctgg cgcgaactgc tcgacggtga cccgacccgt agccgcatct
     3121 acaaggacat cggcctgggc gtggccacgg ccggcatcga gtattacctg ccgctgtttt
     3181 tcgagtcaac cgccagcgtg tttgattacc tgggtgatgc cgccacggtg gtgctgcatg
     3241 gcgatctgga tcaggccttt gcccacttct ggcaagacac gcgcgagcgt taccggctgg
     3301 tgcaaggcga cccggaacga ccggtgctgc cacccgatgc gctgtttttg aacgccgagc
     3361 agttctacaa ccgggtcaat cagcatgcac aactggcgct cagagcggct cgtgttgccc
     3421 cattccagca agaaagtgcg ccgtcattcc cgcaagatag ttcaccgtct attgatctgg
     3481 catcaggtca tgacggtggc ttggcgcagg cctatgccga atttgatgcc ttgccgccac
     3541 tcactgtggt gcgtggcgca gaagacccgc tggtcagcct gaagcggcac atcagccata
     3601 cagcgcagcg tgtattggtg ctggccgaga gtgatggccg ccgcgccagc ctgctcgatt
     3661 tcctgcattc cagcgcattc gacccgccca cgtttgactc gcttgcgggg tttttggcca
     3721 gcgctgaaaa ggtcggcatt gccaccagca acctggccac tggctttagc tggctggaaa
     3781 gcggcgttga ctttgtcacc gaaaccgagc tgtttgctgc cagcccgacc agtcgcagac
     3841 gcaaaaagca agagcaggtc agtgacattg aagcgctgat caaggacctg agtgaactca
     3901 aggttggcga cccggtggtg cattctgccc acggcattgg ccgttaccgc gggctgatca
     3961 acctggacct ggggcaaaag ctggccaacg gagagcctga aattcaggag ttcttgcacc
     4021 tggagtacgc cgacaaggcc acgctgtatg tgccggtgag ccagttacaa ctgatcagcc
     4081 gctacaccgg tgtcagcccc gacgaagcac cgctgcacaa acttggcagc ggccagtggg
     4141 acaaagccaa gcgcaaagcc gccgaacagg tgcgcgacag cgccgccgag ctgctcaaca
     4201 tttacgcccg cagggctgcc cgcgagggtc atgccttccg ctacagcccc aacgactacg
     4261 aagcctttgc caacgacttt ggctttgagg aaaccgccga ccagtgcgcc gccatccacg
     4321 ccgtcattca ggacatgatc agcccgcgcc cgatggaccg gctgatctgc ggcgatgtcg
     4381 gttttggcaa gaccgaagtc gccctgcgcg cggccttcat tgcgatcacc ggcggcaagc
     4441 aggtggcgtt tctggccccc acaactttgc tggccgaaca gcattttcag accctggtgg
     4501 accggtttgc caaatggccg gtgaaagtgg ctgagatgag ccggtttcgc tctggcaagg
     4561 agatcaccgc tgcactcaaa ggcgtggcag acggcaccat tgacattgtg gtgggtaccc
     4621 acaagctgct ttcgcagtcc accaaattca aagacctggg gctgctgatc atcgacgagg
     4681 aacaccgctt tggggtgcgc cacaaagaac agatgaaggc gttgcgcgcc gaggtcgatg
     4741 tgctgaccct gaccgccaca ccgattccgc gcacgctggg catggcgctt gaaggtctgc
     4801 gcgacctgag cgtgatcgcc accgctcccc agcgccgcct ggccatcaag acctttgtcc
     4861 gcaccgaagg caatggcgtg attcgcgagg cggtgttgcg tgaactcaag cgcggtgggc
     4921 aggtttattt tctgcataac gaagtcgaga cgatcgagaa ccgccgggca aaactggaag
     4981 aattgctgcc cgaggcccgt atcgcggtgg cccatggcca gatgccggaa cgtcagcttg
     5041 aagcggtgat gcgcgatttt gtcgcccagc gcagcaacct actgttgtgc tccaccatca
     5101 tcgagaccgg tattgacgtg ccgacggcca acaccatcgt gatgagccgc gccgacaagt
     5161 ttggcctggc gcagttgcac cagttgcgcg gccgggttgg ccgcagccac caccaagcct
     5221 atgcttacct gatggtgcca gacatggaag gcctgaccaa acaggcaacc atgcgcctgg
     5281 acgcaattca ggcaatggaa gaactcggca gcgggtttta cctggccatg cacgacctgg
     5341 aaattcgcgg tgccggtgaa gtgctgggtg aaaaccagag cggcaacatg ctggaagtgg
     5401 gttttcagct ctacaacgag atgctgtccg aagcggtgcg ttgcctgaaa gccggtatcg
     5461 agcccgattt gctcagcccg ctgaacgtgg ccaccgacat caacctgcat gcgccagcgc
     5521 tgttacccga cgatttttgc ggcgacgtgc atttgcgcct gtccttctac aaaaaactgg
     5581 ccaccgccaa gaccaccaac cagattgatg cgctgctgga agaaatcatc gaccgctttg
     5641 gcaaactgcc agcacaggcg caaaccctga tcgacgtgca ccgcctgcgg gtactgagcc
     5701 agccgtatgg ggtgaccaag gttgatgccg cgcccggtgt gatcaccatc accttcagga
     5761 aagacccgcc gattgatgcc atgaaaatca ttgccctgat ccagaaaaac aagcacatca
     5821 gattggctgg caacgaaaaa ctgcgcattg agcgagacct gccagaggcg aaagaccggg
     5881 cacaaatggt gcgtgaggtg ctgcgcaatc tggggcagcc ggtgccgcaa ctggcaagct
     5941 gaaaaggtcg gactgacaaa tgccagtgcc atgccgcgcc tttgacccgg gcaaaatgcc
     6001 gaccggatca gaaagtcttt ggcttggatg gagggtgtgc cgacaaggat tgccggtggc
     6061 ggcaatgcat ctcgcccaca gattcagg
//
LOCUS       DAOSLL010000244         6078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11729_length_6078_cov_3.574631, whole genome shotgun sequence.
ACCESSION   DAOSLL010000244 DAOSLL010000000
VERSION     DAOSLL010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11729_length_6078_cov_3.574631"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /gene="motA"
                     /locus_tag="PLB25_11405"
     CDS             <1..817
                     /gene="motA"
                     /locus_tag="PLB25_11405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HOE42217.1"
                     /translation="FGVYIFHGGNIMVVLKALPFELITIGGATMGAFVANNQMKVIKS
                     SIKGLGMCFKGSKYTKARYMDLLALMYDILQKARKEGLMAIEKDVEEPHSSEIFKKHG
                     DVGHDHHVVEFITDYLRMMVSGNLNAHEIESLMDSEIDTHHAEEHAAVAALQRIAGGL
                     PAFGIVAAVLGVVNTMGSVGQPPAVLGGMIASALVGTFLGILLAYAFAEPLAGLLEQK
                     VEEAGKEFQCIKTTLLASMQGYNPATAIEFGRKVLYSTERPTFGELEAFVKKK"
     gene            836..1774
                     /gene="motB"
                     /locus_tag="PLB25_11410"
     CDS             836..1774
                     /gene="motB"
                     /locus_tag="PLB25_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012836864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HOE42218.1"
                     /translation="MATEAKKLQPIIIKRVKKGGHSAHGGAWKIAYADFVTAMMAFFL
                     LMWLLGSTSEGDKKGLSDYFQSPLKVAMQGGAGAGASQSVVSGGGNDLTQSAGQARRG
                     DANDPKAKRMSGDAAKAAQAKKDAEKLAELSAKISSLISRNPKLAEFSEQIKLSITAD
                     GLQIQIVDDQKRPMFDSGSPSVKPYMREILQEIGAALADVDNKISVDGHTDQTPYGSG
                     ARGYSNWELSADRANATRRELVVAGMPDDKMARVMGLASSILLDEENPRGPANRRISI
                     TVMTREAEERLLGGRKTDIITEQNAAPAPPASAAQQ"
     gene            1844..2233
                     /gene="cheY"
                     /locus_tag="PLB25_11415"
     CDS             1844..2233
                     /gene="cheY"
                     /locus_tag="PLB25_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HOE42219.1"
                     /translation="MANDLKFLVVDDFSTMRRIVRNLLKESGYAEADEAEDGVAALQK
                     LRNGSFDFVVSDINMPNMNGFQLLMEIKKDEKLKHIPVLMVTAEARKEDIVMAAQQGA
                     SGYIVKPFTKATLEDKVNLILTKLGLK"
     gene            2230..2910
                     /locus_tag="PLB25_11420"
     CDS             2230..2910
                     /locus_tag="PLB25_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HOE42220.1"
                     /translation="MSTATSGHVISAVTSPVDVSQDVHQRIGLLTRQLHDSLQGLGLT
                     DKVKALAGEIPDAKSRLTYIARLTGEAAEKVLNNVDQAKSHHDHIAAETRRIGGLIVK
                     DPVAAVAKGHVMNFLTDVEEATQKIDKNLTDIMMAQDFHDLTGQVIAKVVSLAASIEE
                     QLVQLLIVTAPVDTVQKPATVVEPPVTGFVIHDHPVLEGPVVNPEGNPDVVKGQSEVD
                     DLLASLGF"
     gene            3006..4163
                     /locus_tag="PLB25_11425"
     CDS             3006..4163
                     /locus_tag="PLB25_11425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HOE42221.1"
                     /translation="MESSSQDRNLPASERKLQKSRDDGQVARSRDLSHLTVLGGGSLG
                     LMWLGPLMLEHIQRQFSLQLLFDAQAIQDPTTMMTRLGSMAAVGLVVSAAFAAIVSVI
                     AIISTVAVGGWVNSLKPITPDFSRVSPLSGLGNMFTKDKFTEVLKMSFIAGMLLVVGF
                     SYLSSSLETLRSLLLQPSANSIGYLAAWLTKGMGLMLLVVLIVAMVDVPLQNFLHKAR
                     MKMSHQEVKQENKESDGNPQMKSHMRQRQRDISQGNSVKAVPRADFVLMNPTHFAVAI
                     QYDDKTMHAPRVVSKGADLLAFKIRDIAAEHAIPVLQSPTLARALYANAEIDQDIPAS
                     LYTAVAQVLAYIYRLKAAMRGDGPMPGEVPVPFVPPELDPLSKVIAAAQTP"
     gene            4160..>6078
                     /gene="flhA"
                     /locus_tag="PLB25_11430"
     CDS             4160..>6078
                     /gene="flhA"
                     /locus_tag="PLB25_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HOE42222.1"
                     /translation="MNFSVSAFKQWFGNNTALMQGAAAPMLVVAILSMMVLPLPPWML
                     DTFFTFNISVALMVMMVAAYMIKPLDFAAFPAVLLLTTLMRLSLNVASTRVVLLEGHT
                     GPGAAGAVIEAFGHFLIGGNFAVGLIVFSILVVINFVVVTKGAERIAEVSARFALDAM
                     PGKQMAVDADLNAGLIDEKEAKRRRAEVSEEADFFGSMDGASKFVRGDAVAGILILLI
                     NIFGGFAVGVLQHGLTAGQAADTYILLAVGDALVAQIPGLLISVAAAMVVSRVGKDRD
                     LSNQIVNQMFISPKVLGITGIIMGILGVIPGMPHTVFLGVASLLGYGAWMLSHQPPPA
                     EAAPVIQAPSSDTEATWDDLQPVDQLGLELGYRLITLVDKNRQGDLLTRIKGVRRKFA
                     QEVGFLPPPVHVRDNLELKPSGYRITLRGVIVGEGEAFPGMFLAINPGGITTPLIGTP
                     TTDPAFGLPAHWIDSQQKEAAQMAGFTVVDSETVMATHLSHLMQVQAARLLSRTETQQ
                     LVEHVSKQAPKLIEEVVPKMVSIAVFQKVLQLLLEESVHIRDIRTIIESLAEHAGSVT
                     DPGELARRVRIALSPAIVQQIYGATHELNVIAIDPSLERLLVQALGNSSGQGQALDPG
                     VAEVLTQKAAEVALK"
BASE COUNT         1363 a         1679 c         1749 g         1287 t
ORIGIN      
        1 ctttggcgtg tacatctttc acggtggcaa catcatggtg gtgctcaagg cattgccgtt
       61 tgaattgatc acaattggcg gtgccaccat gggcgccttt gtcgccaaca accagatgaa
      121 ggtcatcaag tccagcatca aaggcctggg catgtgtttc aagggcagca agtacaccaa
      181 ggcgcgctac atggacctgc tggctttgat gtacgacatc ttgcaaaaag cccgcaaaga
      241 aggtttgatg gcgattgaaa aggacgttga ggaaccacat tcgtccgaga ttttcaagaa
      301 acatggggac gttggtcatg accatcatgt ggtcgaattc atcaccgact atttgcgcat
      361 gatggtctcg ggcaacctga atgcccacga aatcgaatcg ctgatggaca gcgagattga
      421 cacccaccat gccgaggaac acgctgccgt tgctgccctg caacggatcg ctggcggact
      481 gcccgctttc ggtattgtgg cggcggtgct gggggtggtg aacaccatgg gctcggtggg
      541 acaaccgcct gctgtgctgg gcggcatgat tgcctcggcc ctggtgggca cgtttttggg
      601 tattttgctg gcttatgcgt ttgccgagcc gctggcgggc ctgctggagc aaaaggtgga
      661 agaagcaggc aaagagtttc aatgcatcaa aaccaccttg ttggccagca tgcaaggcta
      721 caacccggca actgccattg agtttggccg aaaagtcctg tactccaccg aaaggcccac
      781 tttcggcgaa cttgaagctt ttgtcaaaaa gaaataacca aggtcgtgta accgcatggc
      841 aaccgaagcc aagaaactcc agccgatcat catcaaacgg gtgaagaaag gggggcactc
      901 ggcccacggg ggtgcctgga agattgccta tgccgatttt gtgaccgcca tgatggcgtt
      961 ctttttgctg atgtggttgc tcggaagtac ctccgagggt gacaaaaagg gtttgtctga
     1021 ctactttcag tcgccgctca aggtcgctat gcagggcggg gccggtgccg gtgccagcca
     1081 aagcgtggtg agcggtggcg gcaatgacct gacgcaatca gcggggcagg ccaggcgcgg
     1141 tgatgcaaat gaccccaagg ccaagcgcat gtctggcgac gcggccaaag cggcccaggc
     1201 caaaaaggat gccgaaaagc ttgctgaatt aagcgccaaa atcagttccc tgatttcaag
     1261 aaacccgaaa ctggccgaat tcagcgagca aatcaagctg agcatcactg cggatggact
     1321 gcaaatccag attgtcgatg atcaaaaacg ccccatgttt gacagcggca gtccgtccgt
     1381 caaaccctac atgcgtgaga tcttgcagga aatcggcgca gcactggcag atgtggacaa
     1441 caaaatcagc gtggatggtc ataccgatca aacaccgtat ggcagcggtg cgcgtggtta
     1501 cagcaattgg gagttgtcgg ctgaccgggc caatgccacc cggcgtgagt tggtggtagc
     1561 aggcatgccc gatgacaaga tggcgcgcgt tatgggcttg gcatcgagca ttttgctcga
     1621 cgaagaaaat ccgcgcggcc cggcaaaccg tagaatcagc attaccgtga tgacccggga
     1681 agcagaagaa cgcttgctgg gtggcagaaa aaccgacatc atcacagagc aaaatgccgc
     1741 gcccgcccca ccagcgtctg cggctcaaca gtaacgcaac ctgtcatcat gatttggaat
     1801 cgacttatgc aagtttcaac cagcgcctcg gagagcctat ctcatggcca atgatctcaa
     1861 atttttggtg gtcgatgatt tctccaccat gcgccgcatt gtgcgcaatt tgctcaagga
     1921 aagtggctat gccgaggctg atgaggcaga agacggtgtc gcagccctgc aaaaattgcg
     1981 taatggcagt tttgactttg tcgtgagcga catcaacatg cccaacatga acgggtttca
     2041 gttgcttatg gagataaaaa aagacgaaaa gctcaagcac attccggtgc tgatggtgac
     2101 cgccgaagcg cgcaaggaag acatcgtcat ggccgcccag cagggcgcat ccggttacat
     2161 cgtcaagccc ttcaccaagg caacgctgga agacaaggtg aacctgattc tgaccaagct
     2221 ggggctcaaa tgagtacagc gaccagcggc cacgtcatct ctgcagttac cagtccggtg
     2281 gatgtgtccc aggatgtcca ccagcgcatt ggtctgctaa cacggcaact tcatgactcg
     2341 ctgcagggct tgggcctgac tgacaaggtc aaggctttgg cgggcgagat tcccgatgca
     2401 aaaagccggt tgacttatat tgcccgcctg actggcgaag ctgcggaaaa ggtgctcaat
     2461 aacgttgacc aggccaaatc ccaccatgat cacattgcag ccgaaacccg gcgcattggt
     2521 ggcttgattg tcaaggaccc ggtcgcagcc gttgccaagg gccatgtgat gaacttcctc
     2581 actgatgtcg aagaggccac tcagaaaatt gacaagaact tgaccgatat catgatggca
     2641 caggactttc atgacctgac cggccaggtg attgccaaag ttgtcagtct ggcagccagc
     2701 attgaggaac agttggtgca attgttgatt gtcactgccc cggttgatac agtgcaaaaa
     2761 ccggcaacgg ttgttgagcc ccctgtgaca ggctttgtca ttcacgacca tcccgtactt
     2821 gaaggaccgg ttgtcaatcc tgaagggaac ccggatgtgg tcaagggcca gtccgaggtg
     2881 gacgatctgc tggccagtct gggtttttga ggcccgacac atcaggcggg caatcacccc
     2941 gcttttcact ctttagttcc agggtcgcac cttgacaatg gggagaacca aggtatctcc
     3001 ccaacatgga atccagcagt caagaccgca atttacccgc atcagaacgt aagctgcaaa
     3061 agtcgcggga tgacgggcag gtggcgcggt cacgcgatct gtcgcacctg actgttctag
     3121 gaggtggttc actaggtctg atgtggctgg ggccgttgat gctggaacac attcagcgcc
     3181 aattcagcct gcaactcctg tttgacgctc aagccatcca ggaccccacc accatgatga
     3241 cccgactggg cagcatggca gcggtcggcc tggtggtcag tgcggcattt gctgccatcg
     3301 tcagcgtgat tgcgattatc agcaccgtgg cagtgggtgg ctgggtcaac agcctcaagc
     3361 ccatcactcc ggacttcagt cgggtcagtc ctttgagcgg cctgggtaat atgtttacca
     3421 aagacaagtt cacagaagtt ctcaagatgt cgttcatcgc gggcatgtta ctggtcgttg
     3481 gcttcagcta tttgtcatcg agcctggaaa ccttgcgctc cctgctgttg caaccttccg
     3541 ccaactccat cggttatctc gcggcatggt tgaccaaagg catgggactg atgctgctgg
     3601 tggttttgat cgtggcgatg gttgatgtgc cattgcagaa ttttttgcac aaagcacgca
     3661 tgaagatgtc gcaccaggag gtcaagcagg aaaacaagga gtctgacggc aacccgcaaa
     3721 tgaagagcca catgcgccag cgccagcgtg atatttcgca gggcaacagc gtcaaagctg
     3781 taccccgggc cgactttgtg ctgatgaatc caacccactt tgcagtcgcc attcaatatg
     3841 atgacaagac catgcacgcg ccgcgcgttg tgtccaaagg cgctgacttg ctggctttca
     3901 aaatcaggga cattgccgcc gaacacgcca ttccggtgct tcagtcacca acgctggcac
     3961 gcgcgctgta cgccaatgca gaaatcgatc aggacattcc ggccagcctg tacaccgccg
     4021 tggcacaggt gctggcttac atctaccgac tcaaggccgc catgcggggc gacggcccaa
     4081 tgcccggcga ggtgccggtg ccgttcgtgc cccctgagct tgatccgttg tccaaagtaa
     4141 tcgctgcggc ccaaacacca tgaacttctc tgtcagtgca ttcaagcaat ggtttggcaa
     4201 caataccgcc ctgatgcagg gtgccgctgc cccgatgctg gtcgtggcca ttttgtcaat
     4261 gatggtcttg ccgctaccgc cgtggatgct cgacaccttc tttaccttca acatatcggt
     4321 tgcgctgatg gtgatgatgg tggcagccta catgatcaag ccgctggact ttgccgcctt
     4381 tccagcagtg ctgctgctga ccaccttgat gcgactgtca ctcaacgtgg catcgacccg
     4441 agtggtgttg ctcgagggtc acaccgggcc gggtgcagcc ggtgcagtca tcgaagcctt
     4501 tgggcatttc ctgattggtg gcaactttgc cgtcggtctg attgtgtttt caatactggt
     4561 ggtgatcaat tttgtcgtcg tcaccaaagg tgccgaacgt attgccgaag tgtcggcacg
     4621 cttcgccctg gatgccatgc caggcaagca aatggcggtg gatgctgatt tgaatgccgg
     4681 attgatcgac gaaaaagagg ccaaacgccg ccgcgctgaa gtgtccgaag aagctgactt
     4741 ctttggctcc atggatggtg cctccaagtt tgtccggggt gacgcagtgg ccggcatact
     4801 gatcttgctg atcaacattt ttggcggctt tgcggtcggt gtcttgcagc acggcctgac
     4861 tgccgggcag gctgccgaca cctacatttt gctggcggta ggcgatgcgc tggtcgccca
     4921 aataccgggc ttgctgattt ctgtagcagc agccatggtg gtgtcgcggg ttggcaagga
     4981 ccgcgacctg agcaaccaga tcgtcaacca aatgttcatt tcccccaagg tgctgggcat
     5041 caccggcatc atcatgggca tcctgggggt cattccgggc atgccccaca ccgtatttct
     5101 tggggtcgct tctctgctgg gctatggagc ctggatgttg tcacatcagc cacctcccgc
     5161 cgaggctgcg cccgtgatcc aggcaccctc gtccgacacc gaggccactt gggatgatct
     5221 gcaaccggtc gaccagcttg ggctggagct gggctatcgc ctgatcactc tggtggacaa
     5281 gaaccgccag ggcgacttgc tgacccgcat caagggcgtg cgccgcaaat ttgcccagga
     5341 agtcggattt ctaccgccac cggtacacgt gcgggacaac ctcgaactca agcccagtgg
     5401 ctaccgaatc acgctgcgtg gggtcatcgt gggtgagggc gaagcctttc ccggcatgtt
     5461 cctggccatc aaccccggcg gcatcaccac cccgttgatc ggcacaccaa ccaccgaccc
     5521 ggcatttggc ttgcccgccc actggatcga cagccagcaa aaagaagccg cgcaaatggc
     5581 cgggtttacc gtggttgatt ccgagaccgt gatggcgaca catttgtcac acttgatgca
     5641 agtgcaggcc gccagattgc taagccgcac agagacgcaa caactggtag agcacgtgag
     5701 caagcaggcc ccgaaactga tcgaagaagt agttcccaaa atggtttcca ttgctgtttt
     5761 ccaaaaggtg ttgcaactgc tgctggagga atccgtccac atccgcgata tccgcacgat
     5821 cattgaatca ctggcggagc atgctggctc ggtcaccgac ccgggcgagc tggcgcgtcg
     5881 ggtgcgcatt gccttgtcgc ccgccatcgt ccagcaaatc tacggcgcta cccacgagct
     5941 caatgtgatc gccattgacc cgtcgctgga acgcttgttg gtgcaggccc ttggcaactc
     6001 atcaggacaa ggccaggcgc tcgacccggg tgtggcggaa gtcctcacac aaaaggcggc
     6061 tgaggtcgcc ctgaagca
//
LOCUS       DAOSLL010000245         6075 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11736_length_6075_cov_5.270266, whole genome shotgun sequence.
ACCESSION   DAOSLL010000245 DAOSLL010000000
VERSION     DAOSLL010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11736_length_6075_cov_5.270266"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1719)
                     /locus_tag="PLB25_11435"
     CDS             complement(<1..1719)
                     /locus_tag="PLB25_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE42223.1"
                     /translation="MNNLKIATRLMLLIGALGAMLLAVGLTGLSGISQSNAALKTVYE
                     DRTIPMGQLGDVQHRLLLNRLAIANSVLDPTPDTLARSIADINANLVTLQQTWDAYMV
                     TYLTPEEAVLAKRFNDDRARLVQQGLLPAVAALRAKDIEGAHRLILEQIRPLFAPVEA
                     GIEALMDLQLKVAKAEYDAAAARYATQRAWSVFAIVAGMLFAVLFGFSLIRGISRSLQ
                     EALAVARAVAQGDLSHAINLKGQDEVAQVLVALSSMQTSLAQVVSTVRQGSEAVASAS
                     AQIAAGNHDLSARTESQASALEQTAASMEQLSATVKQNADNAIQANALALHASATAVH
                     GGEVVGMMVQTMKHIDDSSNKIFDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFA
                     VVATEVRSLAGRSAAAAKEIKTLISASVERVAQGSAMVNQAGVTMGDVVSSIALVTNI
                     MGSISAASREQSLGVSQVSEAVMQMDQVTQQNAALVEEMAAAAGSLKSQALALVETVA
                     VFKLDDSHGDEPMSTHSVVVDAASARRTRQAIHPLDQAVNAYAKDRRAGDARATGLLP
                     ALTCSGC"
     gene            complement(1716..2120)
                     /locus_tag="PLB25_11440"
     CDS             complement(1716..2120)
                     /locus_tag="PLB25_11440"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE42224.1"
                     /translation="MPIQLLIVDDSDLIRTSLRALLGSVAGIAVIREATTLAEALDSA
                     RCDPPTLVILDLNLPDGLGVDIIQQLKQCSPTLWLAVLTIHADPSYRQRCLALGADWF
                     FDKATETDPLLEVVRQLVVQNPTIEPQPRNSP"
     gene            2461..3717
                     /locus_tag="PLB25_11445"
     CDS             2461..3717
                     /locus_tag="PLB25_11445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOE42225.1"
                     /translation="MSTFQINLHEAIYSLSDALDLVGVTHIHHGKRVAFMAAEVGKFL
                     EWPKARLDDLFQAAILHDSGVSKTSVHAKLAQLEWELEGEHCHIGANLLASCALLRPL
                     APMVLHHHTHWTTLQDMVLSPEVKLAANCIYLVDRVDILTLQYVTTGTDILLGKDDIR
                     QQILNHRGDWFCPELVDAFMELSDSEAFWFMLETDHVNGYVATWLAETTQQGMTFEDL
                     SSLMHIFSSIVDAKSAFTKEHSDGVASLARYLGETLQLDEASCDMLELAGLLHDIGKL
                     RVPDQLLEKPGPLTEVERRTMSRHSFDTYNLLCNIKGLEQVALWAAQHHERVNGTGYP
                     YHNRAGGISLQARIIAVADVFQALAQRRPYRGAQSAADIIVILQEQVDIGRLDGQVVA
                     CVADNLQACWRAAMQVMPNSEPVPQT"
     gene            3714..4682
                     /gene="pip"
                     /locus_tag="PLB25_11450"
     CDS             3714..4682
                     /gene="pip"
                     /locus_tag="PLB25_11450"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HOE42226.1"
                     /translation="MNDIAPDTHFFPPIKPFHSGRLKVDDLHTIYWEECGNPGGAPVL
                     FLHGGPGAGLSPEHRQFFDPAFYRIVLFDQRGAGQSTPLGEIRQNTTALLIEDIERLR
                     LLLGIEKWLLFGGSWGSTLALAYGQAHPQRCSGFVLRGIFLGTQAEIDWFFDGIKCFF
                     PKEYEDFVADIPLHEHGDLLQAYARRLFSDDLAQNLHAARTWSRFEGSCLHLLQQPAV
                     AEQFGSDAVALGVGRLEAHYFRNAAFLTDDQLIASIDRIRHLPATIVHGRYDVICPVR
                     SAWRLHQAWPEASFEIIEDAGHAAFEPGIGRALVAATERFKRTHTL"
     gene            complement(4792..5895)
                     /locus_tag="PLB25_11455"
     CDS             complement(4792..5895)
                     /locus_tag="PLB25_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
                     /protein_id="HOE42227.1"
                     /translation="MSIEIRNISKDFGNFHALSDVSLDIESGELVALLGPSGCGKTTL
                     LRIIAGLETADQGHILFNGTDTTDKHVRERNVGFVFQHYALFRHMSVFDNVAFGLRMK
                     SKATRPREAVIRQKVHDLLGLVQLDWLADRYPSQLSGGQRQRIALARALAVEPQVLLL
                     DEPFGALDAKVRKELRRWLRRLHDDLHVTSIFVTHDQEEALEVADRVVLMNSGQVEQV
                     GSPQDVWDHPASPFVYGFLGDVNLFHGRAHEGEMRIGEDQQGLRLATPEHSEVQDSKA
                     FAFVRPHDLDVQRFVAGANPTDQPRGIVARLTRAMVVGPIARLELLPLDASASGSADI
                     IEAQVPAQHYRELGLSEGETVVLTPRRARVFVQ"
BASE COUNT         1193 a         1859 c         1803 g         1220 t
ORIGIN      
        1 gcacccactg catgttagtg ctggcagcaa cccggttgcg cgtgcatcac ctgcacggcg
       61 atccttggcg taagcgttga ctgcttgatc caatggatga attgcctgcc tggtgcggcg
      121 ggcagatgca gcatcaacaa ccacagaatg cgtgctcatc ggctcgtcac catgactgtc
      181 gtcgagctta aacacggcga ctgtttcaac cagcgccagc gcctgcgatt tcaggctacc
      241 cgctgctgcc gccatttctt ccaccagcgc cgcattctgc tgggtcacct ggtccatctg
      301 catcacggct tcgctgacct ggctgacacc caggctctgc tcgcggctgg cagcgctgat
      361 ggagcccatg atgttggtca caagcgcaat gctgctgacc acatcaccca tggtgacacc
      421 ggcctgattg accattgcag agccttgcgc cacccgctcg acactggcac tgatcagggt
      481 cttgatttct ttggccgctg ccgcagagcg cccggccagg ctgcgcacct cggtcgccac
      541 caccgcaaag ccacggcctt gttcaccggc acgggctgcc tccaccgcag cattgagggc
      601 caggatgttg gtctgaaagg caatgccgtc aatcacgctg atgatgtcaa agatcttgtt
      661 cgatgaatca tcaatgtgtt tcatggtctg caccatcatg ccaaccacct cgccgccatg
      721 aaccgccgtg gcgctggcat ggagggccag cgcattggcc tgaatcgcgt tgtcggcgtt
      781 ttgtttgaca gtggcgctga gttgctccat cgaagccgca gtttgctcca gcgcactggc
      841 ctgcgactcg gtgcgggcac tcaggtcgtg gttgcccgcc gcaatctggg cgctggcgct
      901 cgccaccgct tctgaacctt ggcgcacggt gctcaccacc tgggccaggc tggtctgcat
      961 gcttgagagc gccaccaata cctgcgccac ctcgtcctgg cctttcaaat tgatggcatg
     1021 gctcaaatct ccctgggcga cggcacgggc cacggccagc gcctcctgca gcgagcggga
     1081 aatgccccga atcaggctga acccgaacaa gacggcaaac agcataccgg ccacgatggc
     1141 aaagacagac cacgcccgtt gggtcgcata gcgggccgcg gcagcatcgt actcggcttt
     1201 ggcgaccttg agttgaaggt ccatcaaggc ctcgatgcca gcttcaaccg gcgcgaaaag
     1261 tggccggatc tgttccagaa tcaggcggtg cgccccctca atgtctttcg ctcgcaaagc
     1321 ggcaacggcc ggcagcagcc cctgctgcac caatcgggct cggtcgtcgt tgaacctttt
     1381 cgccagcacc gcttcctcgg gcgtgaggta tgtgaccatg taggcatccc aggtctgctg
     1441 cagggttacc aggttggcat tgatgtcggc aatgctcctg gcaagcgtgt caggtgttgg
     1501 gtcgagcacg ctgttggcaa tcgccaggcg attgagcaac agccggtgtt gtacatcgcc
     1561 gagttggccc atgggaatgg tgcggtcttc ataaactgtc ttgagcgccg cgttggattg
     1621 gctgatgccc gacagtccag tcaatccgac ggccagcaac atggcgccca gcgcaccgat
     1681 cagcagcatc aggcgggtgg caattttcag attgttcatg gggagttcct tggttggggt
     1741 tcgatcgtcg ggttctgcac aacgagctgg cgaaccactt ccagcagcgg atcggtttcg
     1801 gtggccttgt caaaaaacca gtccgcaccc agcgccagac agcgctggcg gtaactcgga
     1861 tcagcatgga tggtcagcac ggccagccac agggtgggcg agcattgttt gagctgctga
     1921 atgatgtcaa cgcccagacc atcgggcaga ttcagatcca gaatcaccag cgtcgggggg
     1981 tcgcaccggg cactgtcaag cgcctcggcc agcgtggtgg cctcgcggat gaccgcaata
     2041 ccggctacgc tgccgagcag cgcacgcaag ctggtgcgaa tcaggtcgga atcatcaaca
     2101 atcagtaatt gaatgggcat gggtcatctt gcttgcccca agctgcctgc gctatcgggc
     2161 gatgctgatt tgactgtggg aaaaggacga cccgggctag gaattgccct acagctctgc
     2221 cgccgttttt cagcactgta ttggcctcta gcgcgcgtcc tgtatgcgct tgcagctact
     2281 aattaaatag taaaaaaccc cagcgcgcgg attctgactg gtggcaacgg gtcttgcggc
     2341 gagcgtgcga ctaagctcgt agcttgccct ggcagaccag gcaccctgcc tgggccagaa
     2401 aggtccggcg gccgaccaag ggcataatga attgttgcaa caaaccggct caggcgcagc
     2461 atgtccacat tccagatcaa tctgcatgaa gccatttatt cgctttcaga cgcgcttgac
     2521 ctggtcggtg tgacgcacat ccaccatggc aagcgggtgg cgttcatggc ggcagaagtc
     2581 ggcaagtttc tcgaatggcc aaaggcgcgg cttgacgatt tgtttcaggc ggccatcctg
     2641 cacgacagcg gtgtctccaa aacctcggtg cacgccaagc tggcccaact ggagtgggag
     2701 cttgaaggtg aacactgcca catcggtgcc aacttgctgg ccagttgcgc gctgctgcgc
     2761 cccctggcac ccatggtgtt gcatcaccat acccactgga ccacattgca agacatggtc
     2821 ttgtcgccag aggtcaagct ggctgccaac tgcatttacc tggtggaccg ggtggacatc
     2881 ctgacgctgc aatatgtcac caccggcacc gacatcctgc tcggcaagga cgacattcgc
     2941 cagcagatat tgaaccaccg gggcgactgg ttttgccctg agctggtcga tgccttcatg
     3001 gaactgtccg actcggaggc cttctggttc atgctggaga ctgatcacgt caacggctac
     3061 gtggcaacat ggctggctga aaccacccag caaggcatga cgtttgaaga tctcagcagc
     3121 ctgatgcaca ttttctccag cattgtggac gccaaaagcg cctttaccaa agagcattcc
     3181 gatggtgtcg cgagtctggc gcgttatctg ggcgagacgt tgcagctcga cgaagcaagc
     3241 tgcgacatgc tcgaactggc tggcctgctg catgacatcg gcaaactgcg cgtgcccgac
     3301 caactactgg aaaagccagg ccccttgact gaggttgaaa ggcgcaccat gagccgtcac
     3361 agctttgaca cctacaacct cttgtgcaac atcaaggggc tggagcaggt ggctttgtgg
     3421 gccgcgcagc accacgagcg cgtcaacggc actggttacc cctaccacaa ccgcgccggt
     3481 ggcatctcgc tgcaagcccg catcattgcc gtggccgatg tgtttcaggc actggcacag
     3541 cgcaggcctt atcgtggggc acagtcagcg gcagatatca tcgtcatcct gcaggaacaa
     3601 gtggacatcg gccggctgga cgggcaagtg gtagcgtgtg tggccgacaa ccttcaggcc
     3661 tgctggcgtg ctgccatgca ggtgatgccc aacagcgaac cggtacctca aacatgaacg
     3721 acattgcgcc agacacgcac tttttcccac ccataaagcc ctttcacagc ggccggttaa
     3781 aggtcgatga cctccacacc atctattggg aagagtgcgg caaccccggc ggcgcaccgg
     3841 tactgttcct gcatggcggg ccaggggccg gtttgtcgcc agaacaccgg cagttttttg
     3901 acccggcctt ttaccgcatt gtgctgtttg accagcgtgg tgccggtcag tccacgccct
     3961 tgggtgaaat ccgccagaac accacagcat tgctgattga agacatcgaa cgcctgcgct
     4021 tgctactggg cattgagaaa tggctgttgt ttggcgggtc atggggttca acgctggcgc
     4081 tggcctatgg tcaggcgcat ccgcagcgct gcagtggctt tgtcttgcgc gggattttcc
     4141 tgggcacaca agccgaaatt gactggtttt tcgatggcat caaatgtttt ttccccaaag
     4201 agtacgaaga ttttgtagcc gacataccac tgcacgaaca cggcgacttg ctgcaggcct
     4261 atgccaggcg gttgttcagc gacgatttgg cgcaaaacct gcacgccgcg cgcacctgga
     4321 gccgttttga aggcagttgc ctgcacctgc tgcagcagcc cgcggtggcc gagcagtttg
     4381 gctcggatgc ggtggcgcta ggtgtgggtc ggctggaagc gcactatttt cgcaatgcgg
     4441 catttttgac cgatgaccag ttgattgcca gcatagaccg tatccggcac ctgcctgcga
     4501 ccatcgttca tggccgctat gacgtgattt gcccggtgcg ctccgcctgg cgcttgcacc
     4561 aagcctggcc cgaggcgagt tttgagatca tcgaagatgc cggccacgct gcgtttgagc
     4621 cgggcatagg cagggcgctg gtggccgcaa ccgagcggtt caagcgaacc cacaccttat
     4681 agcggcctgc cctgacctgc cggcagcaac gctggcttga tgccgccagc gggtaacggc
     4741 tccctgaggg gtggcaacaa agcgaattgg tgagcagcgg ccattgccgc actattgcac
     4801 aaacaccctg gcccggcgcg gtgtcaacac caccgtctcg ccctcactga ggcccagttc
     4861 acggtaatgc tgggctggca cctgcgcctc gatgatgtct gcgctgccac tggcactggc
     4921 atcgagcggc aacaactcca gccgggcaat cggccctacc accatcgcgc gggtcagtct
     4981 ggccacaatg ccacgcggct gatctgtcgg gttggcacct gccacaaaac gctgcacgtc
     5041 aagatcatgc gggcgcacaa aggcaaaagc cttgctgtcc tgcacctcac tgtgctccgg
     5101 tgtggccaat cgcaggccct gctggtcttc gccaatgcgc atttctcctt catgcgcacg
     5161 gccatggaaa aggttgacat cgcccaggaa gccatagaca aacgggctgg ccggatggtc
     5221 ccagacatct tgcggtgaac caacctgctc gacctggccg ctgttcatca gcaccacccg
     5281 gtcggccact tccagtgctt cttcctggtc gtgggtgaca aagatgctgg tcacatgcaa
     5341 atcgtcgtgc aggcggcgca accagcggcg cagttccttg cgcactttgg catcgagtgc
     5401 gccaaatggc tcatcgagca gcagcacctg tggctccacc gccagggccc gggccagggc
     5461 aatacgttga cgctggccac ccgagagttg cgacggatag cggtccgcca gccagtcgag
     5521 ttgcaccaag cccagcagat catggacttt ctgccggatg acggcctcgc gtgggcgtgt
     5581 tgccttggat ttcatgcgca gtccaaaggc cacgttgtca aacacgctca tgtgacgaaa
     5641 caaggcgtaa tgctgaaaca caaaccccac attgcgctcg cgcacgtgtt tgtcggtggt
     5701 gtcagtgccg ttgaacagga tgtggccctg gtcggcggtc tccagccccg cgatgatgcg
     5761 caacagcgtg gttttgccgc agccggacgg ccccagcagg gccaccagct cgcccgattc
     5821 gatgtccagc gaaacgtcgc tcagggcgtg gaagttgcca aaatctttgc tgatgttgcg
     5881 gatttcaatg ctcatgatcc tagattcctc agttggacgc gcccgagtgg gtcgctggcg
     5941 gcgcgtctgg gtaggcgcga cgacgctgcg ggctcctgcc cgctaggagg agcaacgccc
     6001 ccaggcgcgt tgccagcggc tcaatcgggt gcgtagcgct gtcacccaac gggtgagcca
     6061 catcgaagct aaaaa
//
LOCUS       DAOSLL010000246         6066 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11769_length_6066_cov_4.369988, whole genome shotgun sequence.
ACCESSION   DAOSLL010000246 DAOSLL010000000
VERSION     DAOSLL010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6066)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11769_length_6066_cov_4.369988"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            787..2865
                     /locus_tag="PLB25_11460"
     CDS             787..2865
                     /locus_tag="PLB25_11460"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE42228.1"
                     /translation="MICPAFALKSRKSKKLKVGNRADLMEELQALRATRLEIDLLLNE
                     SSDPIFSLSANGTYRYANQALAESLGLSVEEIVGKTQWDLFAKEDADRRFADISWVFK
                     NAQVKVVEVRCPGPNGDRHYLTTIKPILNERGLVACVICIAKDISERKQLEQTLQDGE
                     QDFRELFMQSPIPMTLNNMSGVIEAVNNRFVEVFGYTREDIPNIDAWWEKAYPDATYR
                     AEIHAAWEHVRETGLPDKSPILPLEVRFTRKDGKQRTVLISGVFIRDHRLITLDDITE
                     RRLLEADLQFDKIRAEHASRSKSRFLAAASHDLRQPAHALGMFAARLAELPNDPQARH
                     LIGRMEESVRAMQDMLDGFFDISRLDDESMPPKLVACPVATVFEQLRNNFVRAAVEKG
                     LRLRFRPTGAWVQSDPALLQRILLNLVSNAIRYTQQGNILVACRPTNEGKLVRIEVWD
                     SGEGIAPQHHDEIFSEFFQVGNPQRDRTKGLGVGLSIVDRACRLLNHPLSMRSSLGHG
                     TRFSLLVPLAPTTARDRPETSTVKPGWGTLKGLRILLIEDDVLGMEGISSLLSSWGCQ
                     VTPAEGAISARELYQRDASFNLIISDFRLGGGINGIETIRMLHQIAGQPIAACLITGD
                     TDASVKNQALEAGLFFLQKPLRPAKLRSLLRNLLKVAAPGDVTVGTREPNVPTAIQKD
                     ADETRSTVNG"
     gene            complement(2993..4324)
                     /gene="guaD"
                     /locus_tag="PLB25_11465"
     CDS             complement(2993..4324)
                     /gene="guaD"
                     /locus_tag="PLB25_11465"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017523960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HOE42229.1"
                     /translation="MKAIRGAALTFVDDPFRVGGEPSAHYESDALILIKDGRIQAFGP
                     YGAIKDSLPADAALTSYPNSLILPGFIDTHVHYPQTQIIGSYGKQLIDWLNNYTFVAE
                     QQFAQADHGRAVARVFLRECMRVGTTTAMVYCTVYPQSVEVFFEEAEKTGARMIAGKV
                     MMDRHAPAALLDTAQSSYDQSKTLLQKWHGRGRLLYAITPRFAATSSPAQMEAAGALW
                     KEFPSAYLQSHVSENLGEIAWVKALYPRRSGYLDVYDHYGQLGPRAVYGHGIHLSDAE
                     LARCHETGTAIAHCPTSNLFLGSGCLNLDKLKNAVRPVRVGMATDLGAGTSFSPLQTL
                     NEAYKVAHLSGNTLSAWQAFYLITRGAAQALYLDHCIGSIAPGFEADLVVLDLHSTPL
                     IDFRMGYCRSLEEALFIQMTLGDDRATRATYVAGELLYDRERQPHFSGTWC"
     gene            complement(4575..5201)
                     /gene="pssA"
                     /locus_tag="PLB25_11470"
     CDS             complement(4575..5201)
                     /gene="pssA"
                     /locus_tag="PLB25_11470"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HOE42230.1"
                     /translation="MTTNPPPKPFAMIREFQLADWFTLANAICGIGALFSVMSYLQEQ
                     DVMHIYFACGLVVAALVFDVLDGRVARWRHATSTLGRELDSLADVISFGVAPAIIAYG
                     CGMQGFFDRIVLAFFVACGVSRLARFNVTAEALSGGGDKVKYFEGTPIPTSVLLVLIL
                     AFAAWSGAVQQHLWLGQVALYGFKFHPLVLMFAASGSLMISRLRIPKF"
     gene            5853..>6066
                     /locus_tag="PLB25_11475"
     CDS             5853..>6066
                     /locus_tag="PLB25_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42231.1"
                     /translation="MTNPTPERSRFLQALARLLLRLLKSLFGAALALLILFEEWGWEP
                     LQRALAWVGRLPGWRWIEARITALPPH"
BASE COUNT         1284 a         1697 c         1786 g         1299 t
ORIGIN      
        1 ccgggccgtg gctggtattt tttcggcggt cgttggcgct aagcgcagct ttgccgctaa
       61 cagcttttcc aggcactggg cgccagacag ggccaatcgt gggctgaccc ctttgagtca
      121 aagtggagag ttcattgctg agctccctca cagatatgaa tgatgaatgg actcagatcg
      181 gggcatgccg cttcgacctt tgtgcggcga tattgcgctg cccgtcgggt cgaggttcac
      241 gaaaatggtg gaacctgcac cgagtgattt aagctcgggt gacagagttg cccagtgcac
      301 atgaaaatct ttggtatgcg gcagagtctg gtgcctgcgc aatgcccaaa ggatgccaac
      361 ggtcaggctg accgcaaggt gaatggacac tggtttgaat gtctgcggcc attctgtctt
      421 ggccgacttt gcctgggagc accgatggca cgccggactg gcaactttcg gaactgtgga
      481 atcggtagcc agaatcgacc ccggcaaggc cggtttgccc ctgatttgca gtcccgcagc
      541 ccaggtttgg ctgaaaaaaa ttcggtgcga catgggcagt tgcggccggt tcgcactcag
      601 cgctccattg gtccgacagg tgcgctggcg aacctgccag gactgggtcg agtttgaggg
      661 ttctcccgca gttcgagcat tgttccggag tgcgatacta ggccttatgt gcttgaagag
      721 cgtatctgtt gtgttgtcct gtcgccgatc tggaagtcgc cacctgactt aacgaagact
      781 gcagacatga tttgccccgc ttttgccctg aaatccagga aatcgaagaa gctgaaagtc
      841 ggtaaccgcg ccgatttgat ggaggaactg caagctctgc gcgcgaccag actggagatt
      901 gacctcctgc tcaatgagtc gagcgatccc attttttcct tgtcggcaaa tggcacctat
      961 cgttatgcca atcaggcttt ggccgagagc cttggtttga gcgtagagga aatcgtaggt
     1021 aagacgcagt gggacctgtt tgcgaaggaa gacgctgacc ggagatttgc ggacatttcc
     1081 tgggtcttca aaaacgcgca ggtcaaggtg gttgaagttc gctgccccgg acccaatggt
     1141 gatcggcact acctgacgac aatcaaaccg attctgaatg aacggggctt ggttgcatgc
     1201 gtgatctgta ttgccaagga tatcagcgag cgcaagcagc tagagcagac cttgcaagat
     1261 ggtgaacagg attttcggga attgttcatg caatcgccga ttcccatgac gctcaataat
     1321 atgagcggtg ttatcgaggc ggtcaacaac cggttcgtag aagtttttgg ctatacgcgc
     1381 gaggacattc caaacattga tgcctggtgg gaaaaagcct acccggatgc tacctaccgg
     1441 gccgaaattc atgctgcctg ggaacacgtg cgagaaaccg gcttacccga caagtccccc
     1501 attttgccgc tggaagtgcg ctttacccgc aaggatggga agcaacgcac ggtcttgatc
     1561 agtggcgtat ttattcggga tcaccgtctg atcacgctcg acgacattac cgagcgaagg
     1621 ttgcttgaag ctgatttgca gtttgacaaa atcagggcag agcatgccag ccgttccaaa
     1681 tcccgatttc tggccgccgc cagccatgac ttgcgccagc cggcccacgc attgggcatg
     1741 tttgcagccc gcctggccga gttgcccaat gacccacaag cccgccacct gatcggtcgc
     1801 atggaggagt ctgtgcgtgc catgcaggac atgcttgatg gcttttttga tatctcccgg
     1861 ctcgatgatg agtccatgcc accgaagctt gttgcctgcc ccgttgccac cgtttttgag
     1921 caattgcgaa acaacttcgt ccgtgcagcc gttgaaaaag ggctgcgcct tcgatttcgg
     1981 cctactggcg catgggttca aagcgatccg gcgttgctac agcgaatttt gcttaacctt
     2041 gtcagcaacg cgattcggta cacgcaacag ggcaatattc tggtggcctg ccgcccaacc
     2101 aacgaaggca agcttgtgcg cattgaggtg tgggacagtg gcgagggaat tgccccacag
     2161 caccacgacg agatttttag cgagttcttt caggtcggaa acccgcaacg cgaccgtacc
     2221 aagggcctcg gggttggctt gagcattgtt gatcgtgcct gccgattgct caaccatcct
     2281 ttgtccatgc gttcgagtct ggggcatgga acgcggttta gcctgctggt tccgctggca
     2341 ccaaccaccg cgcgcgaccg acccgagacc tccacggtga aaccgggttg gggaacgctg
     2401 aaaggtttgc gtattctgtt gatcgaggac gatgtgctgg gcatggaggg gatttcgagt
     2461 ttgctgtcga gttggggatg ccaggtaaca ccggccgaag gggcaatcag tgcccgagag
     2521 ctttatcagc gcgatgcttc ctttaacctc atcatcagcg atttccgtct gggcggtggc
     2581 atcaatggca tcgaaaccat acgcatgttg caccagattg ccgggcagcc aatagcggct
     2641 tgcctgatca ccggggacac cgatgcgagc gtgaagaacc aggccttgga ggccgggctt
     2701 ttctttttgc agaaaccgct gcgtccggcc aagctgcgca gcctgctgag aaacctgctc
     2761 aaggttgctg cccctggcga tgttactgtt ggcaccaggg agccaaatgt acccacagcg
     2821 attcaaaaag atgctgatga aaccagaagt accgtcaatg ggtgagatca cctgacattc
     2881 ggttgaacca ctggcaggcc gtcggcgttc tttgctgccg ttgaaggcaa aatcatttgc
     2941 ctgccggtcc ggctccaacg cctgcaaagc cccgctggtc gttggcgatt ggtcagcacc
     3001 aggtacctga aaaatggggc tgtcgctcgc ggtcatacag cagttcaccc gcaacatagg
     3061 tggcacgggt ggcacgatca tcgcccaagg tcatctgaat gaataacgcc tcttcgaggc
     3121 tgcggcaata ccccatccgg aaatcaatca gcggggttga atgcagatca agcacgacca
     3181 gatcagcctc aaagccgggg gcaatcgatc caatgcaatg gtcaaggtag agcgcctgcg
     3241 cggcgccgcg ggtgatcaga tagaaagcct gccaggcact tagcgtattg ccactgaggt
     3301 gcgccacctt gtaggcctcg ttcaaggtct gcaatggcga aaaactggta cccgcaccca
     3361 ggtctgtggc cattcccacc cgcactggtc gcacggcgtt tttcagcttg tccagattca
     3421 ggcagccgct gcccagaaac agattcgatg tcgggcaatg ggcgatggcg gtgccggttt
     3481 catggcaacg cgccagttct gcgtcgctaa ggtgaatgcc atgcccgtac accgccctcg
     3541 gtcccaattg cccgtagtgg tcatatacat ccagatagcc gctgcgccgt ggatacagcg
     3601 ctttgaccca agcgatttcg cccaagttct cggacacgtg cgactgcagg taggcgctcg
     3661 ggaattcctt ccacagcgca cctgcggcct ccatttgcgc cggtgagctg gtggcggcaa
     3721 agcgaggggt gatggcgtag agcaggcggc cacgcccatg ccatttttgc aacagggttt
     3781 tcgactggtc gtagctcgat tgggcggtgt cgagcagtgc cgcaggcgca tggcggtcca
     3841 tcatgacctt gccggcgatc atgcgggcac cggttttctc tgcctcttca aaaaacacct
     3901 ccaccgactg cgggtagacc gtgcagtaaa ccatggcggt ggtggtgccg acccgcatgc
     3961 attcgcgcaa aaacaccctt gcaacagcgc gcccgtggtc agcctgggca aactgctgct
     4021 cggcaacaaa ggtgtagttg ttcaaccagt cgatcaactg cttgccataa gagccgatga
     4081 tctgggtttg tggatagtgc acatgcgtgt cgatgaagcc tggcaggatc agtgagttcg
     4141 ggtagctggt caaagccgca tccgcaggca gactgtcctt gatcgcacca tagggcccaa
     4201 atgcctggat gcgaccgtct ttgatcagga tcagggcatc tgactcatag tgcgccgaag
     4261 gttcgccccc aaccctgaac gggtcatcaa caaaagtgag cgctgcgccg cgaatggcct
     4321 tcatgccagt aggtgaagct gtcgtaagct actcctgtga agtttttcaa tggctgaatc
     4381 atcgggacct gtcgatcctg tggtgcatcg gccgcgagcc gactgcagtg gccaaccttg
     4441 cacttgatgt ctggccccaa aatgggctgc gcgccggcaa atccggcaca ggccagcctt
     4501 gctggggtcg cttcttgctg ctcgatgcat aagtctggca ggctcctcgt atggttctgg
     4561 tcgggacagg gtgttcagaa cttgggtatg cgcaagcggc tgatcatcag tgaacccgat
     4621 gcggcaaaca tcaacaccag cggatgaaac ttgaagccat acagggccac ctggcccagc
     4681 cacaagtgct gctgcacggc accgctccat gcggcgaacg ccagaatcag caccagcaga
     4741 accgaggtcg gtatcggtgt gccctcaaaa tatttcacct tgtcgccgcc ccctgacagc
     4801 gcttcagccg tgacgttgaa tcgggccagc cgtgaaaccc cgcaggcgac gaaaaaagcc
     4861 agcacgatcc ggtcaaaaaa cccctgcatg ccgcagccgt aggcaatgat cgccggtgcc
     4921 acaccaaacg agatcacgtc ggccagtgaa tcgagttcgc gtcccagggt tgaggtggcg
     4981 tgccgccacc gggccacgcg cccgtccagc acatcaaata ccagtgccgc caccaccagt
     5041 ccgcaggcaa agtaaatgtg catgacatct tgctcctgca gataggacat gacagaaaac
     5101 agggcaccga tgccgcagat ggcgttggcc aaggtgaacc agtcggccaa ctggaattcc
     5161 cggatcatcg cgaaaggttt gggcggtgga ttggtggtca tcagataaaa cttcctgggt
     5221 gaaatgcgtc ggctgttggg ctgcgtcagc cgcacagcga caggcccaat ggtatcggca
     5281 gattggatca gcccagcggc gcgacaaact gatcgtctgg cccgtacttg aatcggctcg
     5341 attttgtggc atccggcgct ggttgctggt ctggccgatc ccaggtgcga tcagccagtg
     5401 caactggtat gcgccatcgg aattcacagc cggaccagca tgcaagcggt gatgggtcag
     5461 ggttgcggca ttgtgatctg ccccatcggg cgtgtccggt tgttgcctcg acatctggat
     5521 ttctgcctgg cagatcgccc agcaagaacc ttgccgcgcc tcgttgatgc catccagttg
     5581 gtagctgaaa tcatgccgta tcccttgcca gatgtgcgct gatagcttct ttatttatag
     5641 ccgattgggg tctgccgtga agctgatgca tgcccaagca tgagtcttcc gacaccatgc
     5701 cgtcaccgga tcagcatggc agatcggcac cttggtcgga cagaattacg ctggtggagc
     5761 ggattcatca aactcgacag acaattctgg cgaaagcaac agcgaacccg aacgtgcgct
     5821 tacagattcc aagtaccctt aaggtgtcgc ccatgaccaa cccaacgccc gagcgaagcc
     5881 gctttctgca ggctttggcg cgtttgctcc tgcgtcttct caaaagcctg ttcggcgctg
     5941 cattggcgct gttgattctt tttgaagaat ggggctggga gccgctgcag cgcgcgctgg
     6001 cctgggttgg ccgcttgccg ggctggcgct ggatcgaagc ccgtatcact gcgctgccac
     6061 cgcacg
//
LOCUS       DAOSLL010000247         6062 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11775_length_6062_cov_5.171966, whole genome shotgun sequence.
ACCESSION   DAOSLL010000247 DAOSLL010000000
VERSION     DAOSLL010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6062
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11775_length_6062_cov_5.171966"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="PLB25_11480"
     CDS             <1..296
                     /locus_tag="PLB25_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE42232.1"
                     /translation="HVAGDGIVGLAMAGQLQPDVLIIDILLPSIDGATLITTLRSHPQ
                     FKGSQLIVLTSLDTSQLGPYRFALGGVPVVHKPHMVTDLPPLLASLLKASRTP"
     gene            293..652
                     /locus_tag="PLB25_11485"
     CDS             293..652
                     /locus_tag="PLB25_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOE42233.1"
                     /translation="MNAALAPHMTACQQAAAERYFKGSASLCHAFLQAGLAQFPNDMA
                     AIEMALTSHDSAALRRLAHSLKTALGMLGFEAQKQRALKLELAAFEGDWSAATMAWHS
                     LAPDLTHLIGIGQPQAG"
     gene            683..3625
                     /locus_tag="PLB25_11490"
     CDS             683..3625
                     /locus_tag="PLB25_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOE42234.1"
                     /translation="MTEEHVGGATLFPIVGIGASAGGLEAFEAFFRACPVNTGMGFVL
                     VPHLDPGHESLLTEILQRSTAMPVVEARDQITVAPNQVFIIPPNRDMSIMGGVLQLQL
                     PRQPRGQRMPIDGFLRALAEDQAERAIGIILSGTASDGTLGLRAILGAGGICLVQDPS
                     SARYNGMPQSAIAAGYATHILPVEEMPAMLLQLNRQSAYRLSLPGEPPDKTISGINQI
                     LLQLRSATGHDFSLYKKSTIGRRIERRMVQHAIDDLAVYARFLSENPAEIQALFKELL
                     MNVTNFFRDPEAFIVLKTSILPPLLAAKPADSVFRVWVAGCASGEEAYSIAMVLQELQ
                     DEATLTHPLTWGYQIFATDLDDDAIATARAGRYPPNIAQDVTPDRLQHFFIKDPDDRG
                     GFKVKKSLREKVVFAVHSVIKDPPFTRLDCLSCRNLLIYLESEQQDRLIPSFHYALNP
                     DGVLFLSSSESIANHPALFAPLDRKWKFYRAKGTAPRSVNLIRYDLNRTVMQAIETTV
                     PGPAVNAATKSVAELSQRALLKTFAPTSVTTDRHGNILYVHGDASNFLRPPPGLISTQ
                     VVEMARPGLQLELRAALLAAATLAKPTLNREVTLPGDDGGSRVVSLSVRPLPGLSADG
                     ENLLLVSFQDIAVLTVPAARRSRARRGTAPTGIEAVRIGELEREVAYARENLQSTIEA
                     QQASNEELKSANEELQSTNEEMQSANEELETSKEELQSLNEEVLSVNSELNAKVEQLS
                     GIQNDMKNLFDSINTGTLFLDHQLIIRRYTREALKIYPLIAGDIGRPLADIQSNFDSQ
                     SLLSELQAVLDTLVPKEREVRTFDGKWYLARIQPYRTLDNVIEGVVLTFTEVTDFKRL
                     SDAVRRNEAILATAQEIAHLGSWELDLETGMANWSDEMFKLFGYPPSGQPMALEDVLK
                     TLSADDRARVGSAIQNAVANQAPYDITYSITRQDGTRREIRARALPIADASGRVTHLV
                     GTSLDVTP"
     gene            3622..>6062
                     /locus_tag="PLB25_11495"
     CDS             3622..>6062
                     /locus_tag="PLB25_11495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE42235.1"
                     /translation="MTTPDKPTTLRAAAEAQLAATPQPTPARPVADLLYELQVHQIEL
                     EMQNEALRQTQLAFEQQRDRYLDLYEFAPVGYLTLSPEGIITEVNLTGVKLLGRERKA
                     LLQHRFAACVAPEDRDVWMRQFLSVKQHAAPLSVELALQRGDGSVFQAQLEYSPQKVM
                     SGTIAAGGADQRAQRVALFDISERKANEMAYRTLFENSMDAVLLTSPDGGIHAANLAA
                     QKMFDRSEAELCRMGRDGVVDPTDSRLEQALAVRETNGSFQGELTFIRKNGVKFPVEV
                     SSQVFVGKDKRLMTSMVVRDITARLKAANALAKSEERYRLAFEHSFDSININRLSDGM
                     YIEVNQAFLDIMGYAPQDITGRTSLELNVWADPADRQHLVDELRRNSRCHNFEARFRK
                     KNGEVIWGLMSAAVIELDGVPCILSVVRDITDIKTARGLLERHQEQLEQLVEQRTAAL
                     HNAETKYRTVADFAYDWETWVDDAGHWLYCSPACERVTGYRAEEFLAHPALYVEITHE
                     DDRANLQAHLDEGGCIGVHNLEFRIHHKNGELRWIEHVCQGVQDAAGKSLGRRVSNRD
                     ITERKRNEEALRQARDQADAANRAKSTFLANMSHEIRTPMNAVIGFTHMLRRQINEPG
                     HLETLGKIAVAADHLLGVINDILDISKIEADKLVLEKSNFELDPMLRRVASMVVERVR
                     EKGLALIIEVDPGIGLVNGDVTRLSQALLNYLGNAIKFTERGSITLRAAIIDNTANDI
                     LCRFEVADSGIGIAAEHLPRLFQAFEQADSSTTRRFGGTGLGLAINRRLARLMGGDAG
                     AQSTPGVGSIFWLTAR"
BASE COUNT         1272 a         1885 c         1775 g         1130 t
ORIGIN      
        1 tgcatgtggc aggtgacggc attgtgggct tggcaatggc cgggcaattg caacccgatg
       61 tgctgatcat tgacattttg ttaccctcca ttgatggtgc caccctgatc accaccttgc
      121 gatctcaccc gcaattcaaa ggcagccagt tgatcgtgct gacttctctc gatacgtccc
      181 agctcggccc ttacagattt gcactaggtg gtgtgcctgt ggtgcacaag cctcacatgg
      241 ttaccgattt gccgccgcta ttggcaagct tgctgaaagc gtcaagaaca ccatgaatgc
      301 agcgcttgca ccccacatga cagcctgcca gcaggccgcg gcggagcgtt atttcaaggg
      361 ctcagccagc ctttgccacg cgttcctgca ggccggcctg gcgcaattcc caaacgatat
      421 ggcggccatt gagatggccc tgacatccca tgacagtgca gcgttgcgcc gcctggccca
      481 cagtctgaag acggcgctgg gcatgttggg ttttgaagcg caaaagcaac gggcgctaaa
      541 gctggagctg gctgccttcg agggtgactg gtctgccgcc acgatggcct ggcacagttt
      601 ggcgcccgac ctgactcacc tgattggcat cgggcaaccc caggcgggtt aaccagccag
      661 cacttcgcaa agcatcgaat atatgactga agagcatgtg ggcggtgcaa cgctctttcc
      721 catcgtaggc attggtgctt ccgccggcgg gctggaagct tttgaagctt tctttcgtgc
      781 ctgcccggtc aataccggca tgggttttgt gctggtgccg catcttgatc ccggccacga
      841 aagcctgctc accgaaatcc tgcagcgcag cactgccatg ccggttgtag aagcgcgcga
      901 tcagatcact gttgcgccca atcaggtttt tatcatcccg cccaaccgcg acatgagcat
      961 catgggtggc gtgttgcaac tgcagttgcc gcggcagccg cgtggccagc gcatgccgat
     1021 cgatggtttt ttgcgcgcgc tggccgagga ccaggccgaa cgcgccatcg gcatcatcct
     1081 ctcgggcacc gccagcgacg gcacgctggg cctgcgcgcc atccttggtg ccggcggcat
     1141 ttgcctggta caagacccga gcagcgcgcg ttacaacggc atgccgcaga gcgccatcgc
     1201 cgccggttat gccacccata tcctgccggt tgaagaaatg ccggcgatgt tgctgcaatt
     1261 gaacaggcag tccgcctacc gcctgagttt gccgggcgag ccgcctgaca aaaccatcag
     1321 cggcatcaac cagatcctgc tgcaactgcg cagcgccacc ggccacgatt tctcgcttta
     1381 caaaaaaagc accattggcc ggcgcattga gcgacgcatg gtgcagcacg ccatcgacga
     1441 tctggcggtt tatgcccgct ttctcagtga aaatccggct gaaatccagg cactgttcaa
     1501 ggaattgctg atgaacgtca ccaatttctt ccgtgacccc gaggccttca tcgtgctgaa
     1561 gacgagcatt ctgccgccct tgctggcggc caaaccggcc gactctgtct ttcgggtctg
     1621 ggtcgccgga tgcgccagcg gcgaggaagc ctattcgatt gccatggtgc tgcaggaact
     1681 gcaggacgag gccactctca ctcacccact gacgtggggc taccagatat ttgccaccga
     1741 cctcgacgac gatgccattg ccacggcgcg cgctggccgc tacccgccca atatcgccca
     1801 ggacgtgaca ccagatcgcc tgcagcactt cttcatcaag gacccggatg accgcggcgg
     1861 cttcaaggtg aagaagagcc tccgcgaaaa agtcgtgttt gccgtgcaca gcgtgatcaa
     1921 ggacccgccc ttcaccagac tcgactgctt gagttgccgc aacctcctga tctatctgga
     1981 aagcgaacag caggaccgtc tcatccccag ctttcactac gcgctcaatc cggacggggt
     2041 gctgttcttg tccagttcgg agagcatcgc caaccatccg gcgctgtttg ccccgctcga
     2101 ccgcaagtgg aagttttacc gcgccaaagg cacggccccg cgcagcgtca acctcattcg
     2161 ctacgacttg aacaggactg tcatgcaagc cattgaaacc accgttcctg gccccgctgt
     2221 caacgccgcg accaagagtg tcgccgaact cagccagcgc gccctgctga aaacgtttgc
     2281 gccgacctcg gtcaccaccg accgccacgg caacattctt tatgtgcatg gtgatgccag
     2341 caacttcctg cgcccgccgc ccggcctgat cagtacccag gtggtggaga tggcgcgccc
     2401 cggtttgcaa ctggaactgc gcgccgcctt gctggcggcg gccacactgg ccaagccaac
     2461 cctcaaccgg gaagtgacgc tgcccggcga tgacggcggc agccgcgtgg tcagccttag
     2521 cgtgcgaccg ctgccggggc tctcggccga tggcgaaaac ctgctgctgg tgagctttca
     2581 ggacattgct gtgctcaccg tgcctgctgc ccggcgttcg cgcgccaggc gcggcacggc
     2641 accgacaggc atcgaagcgg tgcgcattgg cgagctggag cgcgaagtcg cctatgcccg
     2701 cgaaaacctg caaagcacca tcgaggcgca gcaggcatcg aacgaagagc tcaaatcggc
     2761 caacgaggaa ttgcagtcca ccaacgagga aatgcagagc gccaatgaag agctggaaac
     2821 ctcgaaagag gagctgcaat cgctcaatga agaagtgttg tcggtcaata gcgaactcaa
     2881 tgccaaggtg gagcagttga gcggcatcca gaacgacatg aaaaacctgt tcgacagcat
     2941 caataccggc accctgttcc tcgatcacca actgatcatt cgccgctaca cgcgcgaggc
     3001 gctgaagatt tatccgctga tcgctggcga tatcggccgt ccgctagccg acatccagtc
     3061 aaatttcgac agccagagct tgctgtcaga gctgcaagcg gtgctcgaca cgctggttcc
     3121 caaagagcgc gaagtgcgca cctttgacgg caaatggtat ctggcacgta tccagcctta
     3181 ccgcacgctc gacaacgtga tcgaaggggt ggtgctgacg tttaccgaag tcaccgactt
     3241 caagcgcctg agtgatgccg tgcgccgtaa cgaggccatt ttggccacgg cgcaggagat
     3301 tgcccacctt ggcagttggg aactggacct ggaaaccggc atggccaact ggtcagacga
     3361 gatgttcaaa ctcttcggct acccgcccag cggtcaaccg atggcgctgg aagatgtgct
     3421 caagaccctg agcgcggacg accgggcgcg cgtcgggtcg gcgattcaga acgcagtcgc
     3481 caatcaggca ccctacgaca tcacctattc gatcacccgc caagacggca cacggcgcga
     3541 gatacgtgcg cgcgcgctgc caatagccga tgccagcgga cgagtcacgc atctggttgg
     3601 cacttcgctg gatgtcacgc catgaccacc ccggacaaac ccacaaccct gcgagccgcc
     3661 gctgaggcgc agctcgccgc cacgccgcag ccaacgcctg cgcggccggt ggcggacctg
     3721 ctgtacgaac tgcaggtgca ccagatcgaa ctggaaatgc aaaacgaggc cttgcgccag
     3781 acccagttgg cctttgagca acagcgtgac cgttacctcg atctttacga attcgccccg
     3841 gtaggctacc tcaccctctc accagaaggc atcatcacgg aagtcaacct aaccggtgtc
     3901 aaactgctgg gccgggaacg taaagccctg ttgcagcatc gtttcgcggc gtgtgtcgcg
     3961 cccgaggatc gggatgtctg gatgcggcaa tttctgagcg tcaagcagca cgccgcgccc
     4021 ctgagcgtgg agttggcgct gcagcgcggc gacggttcgg tgtttcaggc gcagcttgag
     4081 tattcaccac aaaaagtgat gagtggcacc attgctgccg gtggcgctga ccagagggcg
     4141 caacgggtgg cgctgttcga catctccgag cgcaaggcga acgagatggc ctatcgcaca
     4201 ttgttcgaaa acagcatgga tgccgtgttg ctgacatcgc cggacggcgg cattcatgcc
     4261 gccaatttgg ccgcccagaa aatgttcgac cgcagcgaag ccgagttgtg ccgcatgggc
     4321 cgcgatggcg tggtcgaccc gaccgattcg cgtctggaac aagcattggc cgtgcgcgag
     4381 actaacgggt ccttccaggg cgaactgaca tttattcgca aaaacggtgt gaagttccct
     4441 gtcgaggttt cgtctcaggt atttgtcggc aaagacaagc ggctgatgac cagcatggtg
     4501 gtgcgcgata tcaccgccag actcaaggcg gcgaatgccc tggcgaagag cgaggaacga
     4561 tatcgactgg cctttgagca cagtttcgat tccatcaata tcaaccgtct gagcgacggc
     4621 atgtatatcg aggtgaacca ggcgttcctt gacatcatgg gttacgcgcc gcaggacatc
     4681 actgggcgca cctcgctgga actcaacgtc tgggccgatc cggccgaccg gcagcacctg
     4741 gttgacgaat tgaggcgcaa cagccggtgc cacaatttcg aagcccgttt caggaaaaag
     4801 aatggtgaag tgatctgggg cctgatgtca gccgcagtga tcgagctgga cggcgtgccc
     4861 tgcatcctct ccgtcgtgcg cgacattacg gatataaaga cggcgcgggg gttgcttgaa
     4921 cggcatcaag aacaactcga acagttggtg gagcagcgca cagccgcgtt gcacaacgca
     4981 gaaaccaagt accgcaccgt cgccgacttc gcctacgact gggaaacctg ggttgacgat
     5041 gccggccact ggctgtattg ctcgcccgcc tgcgaacggg tcaccggcta ccgcgccgag
     5101 gagttcctgg cgcatccggc cttgtatgtc gagatcaccc acgaagatga tcgcgccaac
     5161 ttgcaggctc atttggacga gggcgggtgt attggcgtcc acaatctcga attccgcatt
     5221 caccacaaaa acggcgaact gcgctggatc gaacacgttt gccaaggcgt gcaggatgct
     5281 gcgggcaagt cgctcgggcg ccgtgtatcc aaccgcgaca tcaccgagcg caagcgcaat
     5341 gaagaggcgt tgcggcaagc ccgtgaccag gccgatgccg ccaaccgcgc caagagcacc
     5401 ttcctggcca acatgagcca cgaaatccgc acgccaatga atgccgtgat cggctttacc
     5461 cacatgctgc ggcgccagat caacgagcca ggacaccttg aaacgctggg caagattgcc
     5521 gtcgccgccg atcacctgct gggtgtcatc aatgacatcc ttgatatctc caagatcgaa
     5581 gctgacaagt tggtgctgga aaagagcaac tttgaactcg atccaatgct caggcgagtg
     5641 gcttcgatgg tggttgagcg tgtccgtgaa aagggactgg cactcatcat cgaagtcgac
     5701 cctggcattg gcttggtcaa tggcgatgtc acccggctca gccaggcgct gctcaactat
     5761 ctcggcaacg ccatcaaatt caccgagcgc ggcagcatca cgctgcgcgc cgcgatcatc
     5821 gacaataccg ccaacgacat cctgtgccgc tttgaagtgg cggattcggg catcggcatc
     5881 gccgccgaac atctgcctcg cctgtttcag gctttcgagc aggccgatag ctcgacgacg
     5941 cgacgctttg gcggcaccgg cctgggcctg gccattaacc gccgactggc caggctgatg
     6001 ggcggtgatg ccggcgccca gagcacgcca ggcgttggca gcatcttttg gctgacggcg
     6061 cg
//
LOCUS       DAOSLL010000248         6048 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11803_length_6048_cov_5.551143, whole genome shotgun sequence.
ACCESSION   DAOSLL010000248 DAOSLL010000000
VERSION     DAOSLL010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11803_length_6048_cov_5.551143"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..252
                     /locus_tag="PLB25_11500"
     CDS             91..252
                     /locus_tag="PLB25_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42236.1"
                     /translation="MRERATPGLNALSLLLMFAPAMLALTMMRGAISSTSSAPASDTV
                     NIGVDHDRL"
     gene            486..755
                     /locus_tag="PLB25_11505"
     CDS             486..755
                     /locus_tag="PLB25_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42237.1"
                     /translation="MFKSLTFLAALVLATGCTTQPTINGTSASDCQTACQSACLPVYY
                     EVAVDSWCGNGCDTYPVSHRAANSRPENPRVVRLGTGRLGTCATP"
     gene            770..1417
                     /locus_tag="PLB25_11510"
     CDS             770..1417
                     /locus_tag="PLB25_11510"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HOE42238.1"
                     /translation="MKAGILAVFCAVGVTACTGLPLAENGCADNQMPGPGYGVAVDCP
                     RGSGLSVIGAKSAPGKFSSRMIFLADQLERNVDLKVAGASYLVGSFVDLNNMNTSSPM
                     GRLIAENLMHELQVRSWKIFEPRLMKNFLINDGGEFVLSRDVKQLRDKYHVTGVVVGT
                     YVVSGDYTVVNARVVNIDNGVVVSSAQVQIPSNWFASGLMADTLGRPMKIVGAAQ"
     gene            1414..2016
                     /locus_tag="PLB25_11515"
     CDS             1414..2016
                     /locus_tag="PLB25_11515"
                     /inference="COORDINATES: protein motif:HMM:NF036774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlgO family outer membrane protein"
                     /protein_id="HOE42239.1"
                     /translation="MTRLLQRGVLAIAGLLGLAGCVTNDPQQCTKEKLSALVAVAEVS
                     QVFDDIALGFCPANCVGELATGSGACAESAPTKLVLVPDFVSITNYLPGVAGLYMGEQ
                     MRAALSQRCSSQIYQAEFGRDLKLSEEGLVALTRNPGEVVRDEFRGQDILIGTYSFNG
                     NRLAMFIRKINSTSGVISKMVSKEVVYNCGFMGTMAKIIK"
     gene            complement(2043..3371)
                     /locus_tag="PLB25_11520"
     CDS             complement(2043..3371)
                     /locus_tag="PLB25_11520"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOE42240.1"
                     /translation="MNPISSFFAQHGVHDVECIFADISGYPRGKLMPAASFAAGAELR
                     ICQAIPMQAVTGEYSYHPVFPDSDPDVRLVPDYRTLTLSPWASVPRAVAVHDCVELNG
                     ELCQFAPRSTLKKVLADYAALGLRAVVAPEIEFYLSAANSDPAQVLLPPAVRGGRAEV
                     GSSAFSLNMLNEQAPFWDEFRAAQVTLGINADTWIHEVGATQFEINLLHGDPVAVADQ
                     AFLFKYAAREIALKHGLNAVFMAKPITGSAGSSMHLHISVVDAQGDNIFSLPDGSESE
                     LFGHFIAGLQSFGPELMLLFAPNVNSYRRYVAGSQAPTGMHWGYDNRTTGLRVPASSP
                     QARRVENRVAGADANPYLAIAASLAAGLAGIKAQLKPSPPLASNGYDEAHGLPRSMEG
                     ALEKMQHSAFARQAFGDDFVTGYCAVKALEYANYQAEISTWERRFLLPQV"
     gene            complement(3528..4313)
                     /locus_tag="PLB25_11525"
     CDS             complement(3528..4313)
                     /locus_tag="PLB25_11525"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HOE42241.1"
                     /translation="MNFDLVIAGVGGQGVLTIAAVLDRAANEAGLYVKQSEVHGMAQR
                     GGAVSAFVRMSDQPVASDLIGNGHASLLLSIEPLEALRYIQLLKPDGWIVSDITPMQN
                     VSNYPAMEEVYRVLFSAPRLVALDATRLAQKAGTIKAQNVVALGAAAMFLPLPIELIE
                     QQIKALFGRKGERILNANLHAFRKGDAAARFTQGLLAAGIASDMVARVVSNIHFPPHP
                     VPADLQAAWCQRLLAADAAQLLQQLQQHDELTRLEALADLVAH"
     gene            complement(4310..5953)
                     /locus_tag="PLB25_11530"
     CDS             complement(4310..5953)
                     /locus_tag="PLB25_11530"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HOE42242.1"
                     /translation="MSDLILLGDEAVARAAIDAGITAAYAYPGTPSTEIYQAIEDHAK
                     KHGLPIKGFWSTNEKVALEEGVGVSYAGCRALVAMKHVGLNVAADPFMNVAVSGAHGG
                     LVIVVADDPSMHSSQNEQDSRYFADFAMVPCLEPASQQQCYDMTREAFELSEQLKVPV
                     LLRLVTRLAHSRSQVRLAPPRAQNKPHYVDDPARFTLIPANARRAYAQLIDKQTALQA
                     WSEAHAVNTLTIRSPGKPGILVSGLAWNYLNEALGEAIEGYNLLRIASYPLPVKKIRQ
                     LLDASSEVFVVEEGYPFIERYVSALGLMTERRIRGKLTGDLPRMGELSQDAIKPCFGL
                     AVEPSLQLAGLADVLVGRPPRLCDKCPHSDAYIAIKEVIADLGAGVRVMGDIGCYSLG
                     YLEPHQAIHSCLCMGSSIGMAIGAAHAGMNPSLCVIGDGTFTHSGMAPLLDAARENSN
                     IKVFILDNSIVAMTGGQPTMATDEEVVDLVAGLGVPRKHIRIVEPTAHKKHQTINAIR
                     EELAHTGLSVIIARRACVTYAKEIKEIKAHKLDIAVVAA"
BASE COUNT         1196 a         1847 c         1822 g         1183 t
ORIGIN      
        1 gcatttgcca tgtttgcatg aaccctcctg aattctgatc tgaatggtca gaattcagca
       61 tggtttcgcg tcacgaggga taccggccgc atgcgcgaac gcgccacacc gggcctcaac
      121 gcattgagtc tgctgctgat gttcgcgccg gctatgctgg cgctgaccat gatgcgcggc
      181 gcaataagca gcacgtcaag cgcgcccgcc tcagacaccg tcaacatcgg ggttgatcat
      241 gaccgtttgt aatggccaga agccaaaagg gcttaactct tgcggccaac cgtccgataa
      301 agctggcacg ggcatgactg gctcttggga catcgatcac gaaccccgac acagaccaac
      361 tcaagtgccg caacaggcgg tcatttctgc ccttagcgct tatcccgtca tcgctagaat
      421 cattcatcaa ggtcatgcgt cttcaagaca ggcttcattt gttcttccaa cggtatccaa
      481 aaatcatgtt caagtcgctg acttttctgg ccgccctggt attggcaact ggctgcacaa
      541 cgcagcccac catcaacggc acaagtgcca gtgactgtca aactgcgtgc cagtctgcct
      601 gcctgcctgt gtattacgaa gtcgcggttg actcctggtg cggcaatggc tgtgacacct
      661 atccggtgtc gcaccgggcg gccaacagtc ggccagagaa cccgcgcgtg gtgcgacttg
      721 gcaccgggcg acttggcact tgtgccacac catgatgaag ggataaccca tgaaagcagg
      781 cattttggcg gttttctgcg cggtgggtgt caccgcttgc actggcctgc cactcgctga
      841 gaacggttgt gccgacaacc agatgcctgg cccaggctat ggtgttgccg ttgattgccc
      901 caggggcagt gggctgtcgg tcatcggggc caaatcagca cctggcaaat tttcctcgcg
      961 catgatcttc ctggccgacc agctcgaacg caatgtggac ctcaaagtgg ctggcgcgtc
     1021 ttacctggtc gggagttttg ttgacctcaa caacatgaac acttcatcgc ccatgggccg
     1081 tctgattgcc gaaaacctga tgcatgagtt gcaggttcgg tcgtggaaaa ttttcgagcc
     1141 acggctgatg aagaatttcc tgatcaacga cggtggcgaa tttgtgctca gccgcgatgt
     1201 caagcaactg cgcgacaagt accatgtgac cggggtcgtc gtcggcacct atgtcgtctc
     1261 gggcgattac acggttgtca atgcacgcgt ggtcaacatc gacaacggtg tcgtggtgtc
     1321 aagcgcccag gtgcagattc cgtccaattg gtttgccagc gggctgatgg ctgacacctt
     1381 gggccgaccg atgaagattg tcggcgcggc gcaatgacac ggctgcttca gcgcggcgtt
     1441 ttggccattg cgggcttgct cgggctggcc ggttgtgtca ccaatgaccc acaacagtgc
     1501 accaaggaaa agctgtcggc gctggttgcc gtggcggaag tctcgcaagt gtttgacgac
     1561 atcgcactgg gcttttgtcc ggccaactgt gtcggcgaac tggccaccgg ctcaggcgcc
     1621 tgcgctgagt cggcaccgac caagctggtg ctggtgcccg atttcgtcag catcaccaat
     1681 tacctgcctg gcgtggctgg cttgtacatg ggtgagcaga tgcgcgccgc gcttagccag
     1741 cgctgcagca gccagattta ccaggccgag tttggacgtg acctcaaact cagtgaagaa
     1801 ggtctggtcg ccctgaccag aaaccccggc gaagtggtgc gcgatgaatt ccgcgggcag
     1861 gacattctga ttggcaccta ctctttcaac ggcaaccggc ttgccatgtt catccgaaag
     1921 atcaacagta ccagcggtgt catcagcaag atggtgtcaa aggaagttgt ctacaactgc
     1981 ggcttcatgg gcaccatggc caaaatcatc aaataacagg cgtttaatcc gattctgtcg
     2041 cgtcaaacct gcggcaacaa aaagcgtcgc tcccaagtgc tgatttcagc ctgatagttg
     2101 gcgtactcca gcgccttcac cgcacagtag ccagtgacga agtcgtcgcc aaaggcctgg
     2161 cgggcaaagg cgctgtgctg cattttctcc agcgcccctt ccatgctgcg cggcaggccg
     2221 tgcgcctcgt cgtagccatt gctggccagc ggtggcgacg gcttgagttg cgccttgatg
     2281 cccgccagac ccgctgccag tgaagccgct atcgccaggt aggggttggc atcggccccg
     2341 gcgacccggt tttccacccg ccgcgcttgc ggtgaactgg ccggaacacg caagccggtg
     2401 gtacggttgt cgtagcccca gtgcatgccg gtcggagcct ggctacccgc cacatagcga
     2461 cggtaagaat tcacgttggg cgcaaacagc agcatcagtt ccgggccaaa gctttgcagt
     2521 ccggcgatga aatgaccaaa cagctcactc tcggaaccat caggcaggct gaagatgttg
     2581 tcgccctggg catcgaccac gctgatgtgc agatgcatcg agctgccggc actgccggta
     2641 atcggcttgg ccatgaagac cgcgttgagc ccatgcttga gggcgatttc acgcgctgcg
     2701 tatttgaaca agaatgcctg gtctgccacc gcgaccgggt cgccatgcag caggttgatc
     2761 tcaaactggg tcgcgccgac ctcatgaatc caggtgtcgg cgttgatgcc cagggtgacc
     2821 tgcgcggcgc gaaactcgtc ccagaacggt gcctgctcgt tgagcatgtt caggctgaag
     2881 gcgctgctgc caacttcggc gcggccacct cgcaccgctg gtggaagcag cacctgcgcc
     2941 gggtcgctgt tggcggcgct caggtaaaac tcgatttcag gggccaccac cgcccgcaag
     3001 cccagcgcag catagtcggc cagcactttt ttcaacgtgc tgcgcggcgc aaactggcac
     3061 agctcgccat tgagctcgac gcaatcgtgc accgctaccg cgcgcggcac gctggcccag
     3121 ggcgacaggg tgagcgtgcg gtagtccggc accaggcgca catcggggtc gctgtccgga
     3181 aacaccgggt ggtaggaata ctcgccggtg acggcctgca tcgggatggc ctggcagatg
     3241 cgcagctcgg caccggcagc aaaactggcc gcaggcatca gcttgccgcg cgggtagccc
     3301 gaaatgtcgg caaagatgca ctcgacatca tgcacgccgt gctgggcgaa aaagctgctg
     3361 atggggttca tgggggtccg gttttacaaa gccgcgcaag acactgctga tgcgctcaga
     3421 aataaatgag aaatatggct ctagcccttt tgcagtatgc gctggcagct atcataataa
     3481 tatcaatttg atcgcctgcc aatgacctgc caaaggccac cgactggcta atgcgccacc
     3541 agatcagcca atgcttcaag ccgcgtcaac tcgtcatgct gttgcaactg ctgcagcaac
     3601 tgcgctgcat cggccgccaa caggcgctgg caccaggcgg cctgcagatc ggcgggtacc
     3661 gggtgcggcg gaaaatggat gtttgacacc acgcgcgcca ccatgtcgct ggcaatgccg
     3721 gccgccagca gcccctgggt gaagcgggcc gccgcatcgc ccttgcgaaa agcgtgcaga
     3781 ttggcgttga ggatgcgctc gcccttgcgc ccgaacagcg ccttgatctg ctgctcgatc
     3841 agttcaatcg gcagtggcaa aaacatggca gccgcaccga gcgccaccac gttttgcgcc
     3901 ttgatggtgc cggccttttg cgccagccgg gtggcatcga gcgccaccag ccggggcgct
     3961 gaaaacagca cccggtacac ctcttccatg gcgggatagt tgctcacgtt ctgcatcggc
     4021 gtgatgtcgc tgacgatcca gccatctggc ttgagcaact ggatgtagcg cagcgcctcc
     4081 agcggttcaa tcgacagcag caggctggcg tggccgttgc cgatcaggtc gctggccacc
     4141 ggctgatccg acatgcgcac aaaggccgac acggcaccgc cgcgctgcgc catgccgtgc
     4201 acctcagact gcttgacata caggccagcc tcgttggcgg cgcggtccag caccgccgca
     4261 atggtcagca caccctggcc accgacaccg gcaatcacca gatcaaagtt catgccgcca
     4321 ccactgcgat gtcgagcttg tgtgccttga tttccttgat ctccttggcg taggtgacgc
     4381 aggcgcgccg ggcaatgatc accgacaggc cggtgtgcgc gagttcttca cgtatggcgt
     4441 tgatggtctg gtgcttcttg tgtgcggtgg gttcaacaat gcggatgtgt ttgcgcggca
     4501 cgcccagccc ggcgaccagg tccaccacct cttcgtcggt ggccatggtc ggctggccac
     4561 cggtcatggc gacaatgctg ttgtcgagga tgaacacctt gatgttgctg ttttcacgcg
     4621 cggcatcgag cagcggtgcc atgccggaat gggtaaaggt gccgtcgcca atcacgcaca
     4681 ggctggggtt catgccggcg tgcgccgcgc caatcgccat gccaatcgac gagcccatgc
     4741 acaggcagga atggatcgcc tggtgcggct ccagatagcc cagcgaatag cagccaatgt
     4801 cgcccatcac ccgcaccccg gcaccgaggt cggcaatcac ttccttgatg gcaatgtagg
     4861 cgtcgctgtg cgggcacttg tcacacaaac gcggcgggcg accaaccagc acgtcggcca
     4921 gcccggcgag ttgcagcgat ggctcgaccg ccaggccaaa gcagggtttg atcgcgtcct
     4981 ggctgagctc gcccatgcgc ggcaaatcgc cagtgagctt gccgcgaatg cggcgctcgg
     5041 tcatcaggcc cagcgcgctg acatagcgct caatgaacgg gtagccttct tccaccacga
     5101 acacctcgct ggaggcatca agcaactggc gaattttttt caccggcagc gggtagctgg
     5161 caatgcgcaa caggttgtag ccttcgatgg cctcgccaag cgcctcattg aggtaattcc
     5221 aggccaggcc gctgaccaga atgccgggtt tgccagggct tcgaatggtc agggtattga
     5281 ccgcatgcgc ctcgctccag gcttgcagcg cagtctgctt gtcgatcaac tgcgcatagg
     5341 cgcgccgggc gttggccgga atcaacgtga aacgcgccgg gtcatcaaca tagtgcggtt
     5401 tgttttgtgc gcgcggcggg gccagcctga cctgcgagcg gctgtgcgcc aggcgcgtca
     5461 ccaggcgcag cagcaccggc accttgagct gctcggacag ctcgaacgcc tcgcgcgtca
     5521 tgtcgtaaca ctgttgctga ctggctggct ccaggcacgg caccatggca aagtcggcaa
     5581 agtaacgcga atcctgctcg ttttgcgagc tgtgcatcga agggtcatcg gccaccacaa
     5641 tcaccagccc gccgtgcgca ccggacaccg ccacgttcat gaacgggtca gccgccacat
     5701 tcagcccgac atgtttcatc gccaccagcg cgcgacagcc tgcgtaagac acgccaacac
     5761 cttcttccag cgccactttt tcattggtgg accaaaaccc cttgatcggc aggccgtgct
     5821 ttttggcgtg gtcttcaatc gcctggtaaa tctcggtcga aggggtgccg gggtaggcgt
     5881 aagcggcggt gatgccagcg tcaatggcgg cgcgggcaac cgcttcatcg ccgagcaaga
     5941 tcaggtcaga catggtgatt ccagttggaa aaagagggtc aatgataaag ccataaagcc
     6001 aatgtcccgg tgcagcaagt tgcagcaccg gggcgcgctg gcatccgt
//
LOCUS       DAOSLL010000249         6044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11818_length_6044_cov_4.958090, whole genome shotgun sequence.
ACCESSION   DAOSLL010000249 DAOSLL010000000
VERSION     DAOSLL010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6044
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11818_length_6044_cov_4.958090"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="PLB25_11535"
     CDS             complement(<1..546)
                     /locus_tag="PLB25_11535"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HOE42243.1"
                     /translation="MALPLALANTKRLIPNFSDIVSQPLPIIHVDQLQPGVFVSLELG
                     WLDHPFLVNSFLIKDADQLAALRELGLSHFSYDPQRSTNKPLPLDGEAATDIPAHITA
                     ANRRLMDEKRGRTGRMAEHRERTLAFEKEYEEAVGSTKDVLTDMLRQPQRAVAQGIAL
                     AQRLTSAFLDDSGATVMMVASH"
     gene            complement(723..1286)
                     /locus_tag="PLB25_11540"
     CDS             complement(723..1286)
                     /locus_tag="PLB25_11540"
                     /inference="COORDINATES: protein motif:HMM:NF018095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HOE42244.1"
                     /translation="MKYLTILGLLGAAVLAAPVASYAQHVGYATKSVNLRAGPARDYP
                     VVFVATRGAPLQVLGCLNGYQWCDVAVGRNRGWVYANNISYPYQGHNAPMIRIGAAVG
                     IGIIAFSVGSYWDNHYRSRPWYGQRSRWVGRPVLARPPQARPPHVRPPHARPAQVRPP
                     VANHRPTPRPPSAHPPGAGRPIPLPAR"
     gene            complement(1638..1847)
                     /locus_tag="PLB25_11545"
     CDS             complement(1638..1847)
                     /locus_tag="PLB25_11545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HOE42245.1"
                     /translation="MITYLSAVALRQGIELDSALRAPPIMPNTCCGRGCNGCVWEYYF
                     AALMRWRDDALALLSGAAMPTAAVG"
     gene            complement(1902..2138)
                     /locus_tag="PLB25_11550"
                     /pseudo
     CDS             complement(1902..2138)
                     /locus_tag="PLB25_11550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914053.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1653 domain-containing protein"
     gene            2363..3175
                     /locus_tag="PLB25_11555"
     CDS             2363..3175
                     /locus_tag="PLB25_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/gamma crystallin-related protein"
                     /protein_id="HOE42246.1"
                     /translation="MKTQLKNIIALATLAIATQAVAQVTFYEQDYFRGQSFTTSRQVN
                     NFDRFGFNDRASSAVVTGNRWEVCEDYRFGGRCVVLRAGRYPSLAAMGLNDRVSSARL
                     IAQNARVDDDRYAPAPVNRPDFRRQNNERLYQADVISVRAVVGPPDQRCWIEREQVGV
                     ERGASNAPGALAGALLGGVLGHQVGGGRGKDLATVGGAIAGAAIGNQVGRNNNAPQVV
                     TRDVQRCQEIPSQARPDFWDVTYTFRGQEHSVQLTAPPGPTLTVNRLGEPRT"
     gene            3279..3434
                     /locus_tag="PLB25_11560"
     CDS             3279..3434
                     /locus_tag="PLB25_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011872192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3096 domain-containing protein"
                     /protein_id="HOE42247.1"
                     /translation="MNIHLSLTPLISLVAGILILIMPKLLNFIVAIYLIVIGLIGLFG
                     SGAFRQF"
     gene            3630..5453
                     /gene="glsA"
                     /locus_tag="PLB25_11565"
     CDS             3630..5453
                     /gene="glsA"
                     /locus_tag="PLB25_11565"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HOE42248.1"
                     /translation="MQSPIQTYLEDLHKSLVGLTAGELASYIPELTKANPDWFGISLV
                     TMDGVAYNVGDTGQLFTIQSVSKPFVYATALADRGVETVSRKIGVEPSGDAFNSISLD
                     PQTGAPLNPMINAGAIATTSLVAGDSAEAQWQRLSASMAAFVGRDLGIDDSVYHSESA
                     TGFRNRAIAWMLKNFGIIDGDPMAALENYFRQCSFEVSCQDLACMAATLANGGVHPVT
                     GRRALPAEHVERVLSVMATCGMYNYAGSWLFEVGMPAKSGVGGGIIAVLPGRFGIGIF
                     SPKLDAKGNSARGIEVCKRLSKDFSLHVFGRAGHPSMALGRVYSAIDAPSRRQPSSAM
                     RSYLMEHARRIKYLCLHGYLAVDGIEYIIRKMLQMASDTHSFILDLHQVDGVSDCAAR
                     LLNEARLGFSRDGIAVVFTRIHARKSISQPLRRVSAKGDRGYLSFEDNDLAVEWCENR
                     LFGEAAHSPVTAASLADFLLFKGMPLVLTLQVEAATTSQVYAIGEEIVTAGQAGDGRM
                     YFIESGQVSVLVPLADGAHHRITSLSAGMNFGEMVLLGQTSRTATVCADTEVRCRILE
                     SRKLEQMAAESPLLKIILLENLAKDMANSLRRATQWIAALA"
     gene            complement(5608..>6044)
                     /locus_tag="PLB25_11570"
     CDS             complement(5608..>6044)
                     /locus_tag="PLB25_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42249.1"
                     /translation="HDPQRVKAKVATTAATQPTSTSVRQAAIQIATAAKMLNDLRENW
                     LNPPEWTHKVPEIIPLGMTESPYPDRIEPKPGICEADRKALQKRTPTNLYNAQPAWLK
                     MAHQQLDQAVAAAYGWTDYSPELPDDDILKRLLALNLARSNG"
BASE COUNT         1124 a         1800 c         1907 g         1213 t
ORIGIN      
        1 gtgggaggcc accatcatca cggtggctcc cgaatcgtcc agaaaagccg aggtcaatcg
       61 ctgcgccagg gcgatacctt gcgctacggc acgctgcggc tggcgcagca tgtcggtcag
      121 aacatctttg gtggaaccga cggcctcttc gtattccttc tcaaatgcca gggtgcgctc
      181 gcgatgctcg gccatacgcc cggtgcgacc gcgcttttca tccatcaatc ggcggtttgc
      241 cgcggtgatg tgcgcgggga tgtcggtggc ggcttcgccg tcaaggggca gcggcttgtt
      301 cgttgaacgc tgtggatcgt aagaaaagtg actcaacccg agttcccgca gggccgccag
      361 ttggtcggca tccttgatca agaacgagtt gaccagaaac gggtggtcca gccagcccag
      421 ctcaagcgac acaaacacgc ctggctgcaa ctggtcaacg tggatgatcg ggagtggttg
      481 cgacacgatg tctgagaaat tcgggatcag gcgttttgta ttggccaggg ccagcggcag
      541 ggccatcgac caacgggaaa cccctggcca gcccagcgca aactcagggt aggttctctc
      601 tcatcggatc tcggtcgttt tggggttcgc gggaatcagt gcgcaaatca acgccttcga
      661 ggaaaagcag atttgccctg gcagtagtcc ggctggggca actgcggtcg cgaactctgt
      721 ggctacctgg ccggcagcgg aatcggtctg cctgcgcctg gcgggtgcgc cgatggcggg
      781 cgcggcgtcg gccgatggtt ggccaccggc gggcggactt gagccgggcg ggcgtgtggc
      841 gggcgcacat gcggtgggcg ggcttgtggc gggcgggcaa gcactggccg accgacccag
      901 cgcgagcgct gcccatacca ggggcgcgac cggtagtggt tgtcccaata gctgccgacg
      961 ctgaaagcga tgatgccaat gcccaccgca gcaccgatgc ggatcatcgg agcgttgtgg
     1021 ccctggtagg ggtagctgat gttgtttgca tagacccagc cacggttccg gccgacggcg
     1081 acatcacacc actggtagcc gttcaggcaa ccgagcacct gcagtggcgc accacgcgtc
     1141 gcaacaaaga caaccgggta gtcccgcgcc ggaccggcac gcaggttgac ggacttcgtg
     1201 gcatacccca cgtgctgtgc ataagaagcc accggagctg ccagcacggc ggcaccaagc
     1261 aggccaagaa ttgtcaaata cttcaatgga tacctcatga gtggtaactg ccaggcaaaa
     1321 aaaccgtgct gcccatcagc acagtggccc cgattatcca catccatgct gcttgcctga
     1381 aaggctgcca aggtgccaag gcagacacgc ccaaaccagg cgttgccacg cccggttgac
     1441 ctgtttgctg cgcttcaggt tactataaaa aaaggagcta tcagcgcttt attggcaagg
     1501 gctggaggca taaaacaggg tcaaaaattc agcttgtggc aagctgttgc gggacacgca
     1561 aagtccgcat ctgtgtcgca gcggcatctg ttcggcgctg atgcgctggc tggcgcggtt
     1621 cagccagcga gcgccggtta cccgactgca gccgtgggca tcgcagcgcc tgatagcaag
     1681 gcgagtgcat catcgcgcca gcgcatcaac gcggcaaagt aatattccca gacgcagcca
     1741 ttgcaaccgc gcccgcagca ggtgttgggc atgatcggcg gggctcgcaa ggcgctgtcg
     1801 agttcgatgc cctggcgcag cgccacggcg ctcaggtagg tgatcagcgc ctgcgccgtg
     1861 gccaggtctt gcagcgccag cacggccggg tcgtggcgca caaagcgggc ttgctccttg
     1921 ccctgaaaga caccggtttg tgcaaacata gccgcgggcc gcacccacag accctgactg
     1981 ccgtacaagg cccggtacag cgtcatgccc tgcagggttt cactgcagcg cacggtatcc
     2041 atcacctcgt accagccgcc cttgtaatgg cggtacaggc cgggcggcgt ggcgtgcagc
     2101 gggggtagct cttggggttc ggtgctggtt tggttcatgg cggctggatt gtccccgata
     2161 ccgactgcgg cattgacagg ccaattacca ggccaattgc cagatcaatc gccagatcaa
     2221 ttgcaagacc tgtgttcgca ggcgtacaga ccgcatgcaa gtgaaacaac atagtcaacg
     2281 ccttggtacc ggactgctat gctggcagga cggatctggc gcggacttgc gtccctgcca
     2341 caactttcta caggagccat tcatgaagac acaattgaaa aacatcattg cgctagccac
     2401 gctggcgatt gccacgcagg cggtagccca ggtcaccttt tacgagcaag actactttcg
     2461 ggggcagtca ttcacaacca gtcggcaggt caacaatttt gaccgtttcg gcttcaatga
     2521 ccgtgcttcg tctgccgtgg taaccggcaa ccggtgggaa gtctgcgaag actaccggtt
     2581 tggcggtcgc tgcgttgtgc ttcgcgctgg ccgctacccg tcactggcgg cgatgggcct
     2641 gaacgaccgc gtctcatcgg ctcgcctgat tgcccagaac gcccgcgttg acgatgatcg
     2701 ctatgcaccc gcgccagtta accggcccga ctttcgccgt caaaacaacg aacgccttta
     2761 ccaggcagat gtgatttcgg tgcgcgcggt ggttgggcca cccgaccagc gctgctggat
     2821 cgagcgcgag caagtggggg ttgagcgggg cgcgtccaac gctcccgggg cccttgccgg
     2881 tgccctgctc ggcggcgtgc tggggcacca ggtgggcggt ggccgcggca aggacctggc
     2941 aaccgtcggt ggtgccattg ccggtgctgc gattggcaac caggtcggcc gcaacaacaa
     3001 tgcaccgcag gttgtcaccc gcgatgtgca gcgttgccag gagataccca gccaggcgcg
     3061 gcccgatttc tgggatgtca cctatacctt tcggggccag gaacatagcg ttcagttgac
     3121 cgcgccaccc ggccccaccc tgaccgtcaa ccggttaggt gagccccgca cctgatactg
     3181 acgcttgcgt cctggcaagc gctgtacggt atccgggtgt ccggcagtgt gatgccggac
     3241 actatggctt tttttcaccc attggcacca aggaccccat gaacattcac ctgagtttga
     3301 caccgctgat ctcactggtg gctggcatcc tgattttgat catgcccaag ctgctgaact
     3361 tcatcgtggc catctacctg atcgtgatcg gactgattgg cctgttcggc agcggggcgt
     3421 ttcggcaatt ctgaggtggc cgttgctggc acctttggcg caggccagtg ccagcttcat
     3481 ccggttggct ggctgcggca gtggcgctct ggcaggctgc cttttggaaa ctgatgtttg
     3541 tttgcggcga aattcgactc tgaccccaat ttccaccggc ttgcccggca tggcttttgc
     3601 gatggcagct acacctcaag agctgccaca tgcaatcacc tattcaaacc tatctcgaag
     3661 accttcacaa aagcctggtc ggtctgaccg ccggtgagtt ggccagctac atacctgaac
     3721 tgaccaaggc caatccggac tggtttggca tcagcctggt aaccatggac ggtgtggctt
     3781 acaacgtcgg tgacaccggt cagttgttca ccatccagtc ggtctcaaaa ccctttgttt
     3841 atgccacggc gctggcggat cggggtgtcg aaaccgtgtc gaggaaaatt ggcgtcgaac
     3901 ccagtggcga tgcgttcaac tcgatcagcc tcgatcccca aactggtgca ccgctgaacc
     3961 cgatgatcaa tgctggtgcc atcgccacca ccagcctggt ggccggtgac agtgccgaag
     4021 cacagtggca gcgcctgtct gcctcgatgg cggcgtttgt tggccgcgac ctcggcattg
     4081 acgactcggt ctaccattct gagagtgcca ccggcttcag gaaccgggcg attgcctgga
     4141 tgctgaaaaa tttcggcatc atcgacggcg acccgatggc cgcgctggaa aactatttca
     4201 ggcaatgctc gtttgaagtc agttgtcagg atctggcctg catggccgcc acgctggcca
     4261 atggcggcgt gcatccggtg accgggcggc gcgccctgcc agccgagcat gtggagcggg
     4321 tgctcagcgt gatggccacc tgcggcatgt acaactacgc cggcagttgg ctgtttgaag
     4381 tgggcatgcc ggccaagagc ggggttggtg gcggcatcat tgcggtgttg ccggggcgct
     4441 ttggcattgg cattttttca cccaaactcg atgccaaggg caacagcgcg cgtggcatcg
     4501 aagtctgcaa gcgcctgtcc aaggacttta gcctgcatgt gtttggccgc gccggtcacc
     4561 ccagcatggc gttgggccgg gtttacagcg ccatcgacgc gccgtcgcgc cgccagccct
     4621 cgtcggcaat gcgcagctac ctgatggaac acgcccggcg catcaaatac ctgtgcctgc
     4681 atggctacct ggctgtcgac ggcatcgagt acatcatccg caagatgctc cagatggcct
     4741 ctgacacgca cagcttcatt ctcgacctgc accaggtgga cggggtctcc gattgcgccg
     4801 cccgcctgct caacgaggcc aggcttggtt ttagccggga tggcatcgcg gtggtgttca
     4861 cccgcatcca cgcccgcaag tcgatctcgc agccattgcg ccgggtaagt gccaagggtg
     4921 atcggggtta cctgagtttt gaagacaacg atctggcagt ggaatggtgc gaaaaccgcc
     4981 tgtttggtga agctgcgcac agcccggtga cggctgcatc gctggcagac ttcctgttgt
     5041 tcaagggcat gccactggtc ctcacgctgc aggtggaagc cgccaccacc agccaggtct
     5101 atgccatcgg cgaggagatc gtcaccgctg ggcaggccgg tgacggccgg atgtatttca
     5161 tcgagtccgg ccaggtcagc gtactggtgc cactggccga cggtgcccat caccgcatca
     5221 cctcactgag cgcgggcatg aatttcggtg aaatggtgtt gctcggccag acatcccgca
     5281 ctgccacggt atgcgccgac accgaggtgc gttgccgcat tctggagtcg cgcaagctcg
     5341 aacagatggc agccgagtca cccttgctga aaatcatcct gctggaaaac ctggccaaag
     5401 acatggccaa ttcgctgcgg cgtgccaccc agtggattgc cgcgctggcg tagcaggcgc
     5461 agcaggtgcc agcgtattgc caattgctgg cccatgctgt gatagaccac cgcgtggttt
     5521 cgctgcaccg ctgcctcgca ggtgcctttc aaatcccagg caacaactcg agactggcac
     5581 gctatttccc cgaattgacc ggtgcggtca gccatttgag cgcgccagat tcaaggccag
     5641 caggcgtttc aggatgtcgt cgtcaggcag ttcggggctg tagtcagtcc agccgtaagc
     5701 agcggctacc gcttggtcaa gttgctggtg cgccatcttt agccaggccg gttgggcgtt
     5761 gtaaaggttg gtgggggtgc gtttttgcaa agccttgcgg tctgcttcgc agatgccggg
     5821 tttgggctcg atacggtccg ggtaaggcga ctcggtcatg ccaagcggga tgatctcggg
     5881 cactttgtgc gtccactcgg gcgggttgag ccagttttca cgcagatcat tgagcatttt
     5941 ggcggctgtg gcgatctgga tggctgcctg gcggacgctt gtgcttgttg gttgcgtggc
     6001 cgcagtggtg gccactttgg ctttgacgcg ctgtgggtcg tggg
//
LOCUS       DAOSLL010000250         6041 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11827_length_6041_cov_4.348480, whole genome shotgun sequence.
ACCESSION   DAOSLL010000250 DAOSLL010000000
VERSION     DAOSLL010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6041
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11827_length_6041_cov_4.348480"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLB25_11575"
     CDS             complement(<1..340)
                     /locus_tag="PLB25_11575"
                     /inference="COORDINATES: protein motif:HMM:NF025305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4212 domain-containing protein"
                     /protein_id="HOE42250.1"
                     /translation="MRLWLLLAWFLASFGVVFFAHDLQQVIAGWPLAYWFAAQGSVLV
                     FIALVAIYAWFANRADGIEPINDPGYGLYLRRIHRRFIAYVILFLLLLLLLALAERAG
                     LRKIWVGAIFL"
     gene            343..774
                     /locus_tag="PLB25_11580"
     CDS             343..774
                     /locus_tag="PLB25_11580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42251.1"
                     /translation="MKRLQLAGALAARIRGSSTDRDDIHGRPTQLMPTTVWYRHNSHF
                     FPANRVNGRHSQVFKGHDHSGRSKQSRHRNNSSTQNTGSGKQAVAGACLDGTHLFGLP
                     LVPAEPGQHLKVDRIDLIVGFKKSYKSPPQCPGKGANGDVL"
     gene            1110..1856
                     /locus_tag="PLB25_11585"
     CDS             1110..1856
                     /locus_tag="PLB25_11585"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HOE42252.1"
                     /translation="MKAQRFEVIDALRGLAVVWMTFFHLGFDLNHFGYIRQNFYADPL
                     WTWQRTLIVSLFLFCAGLGQAVALAQGHGWARFWQRWVQVVACALLVTLGSWLMYPGS
                     FIYFGVLHGLALMLLLARLTCHWGRWLWWLGWLAITSVLIAPYAMNSWATSQLIELFN
                     APMLNWIGWITEKPVTEDYVPLLPWLGLVWWGLATGKWLLDTKPGVLRQALAPALKPL
                     AWLGRWSLSYYMLHQPVLIGGLMLASWLVK"
     gene            complement(1860..2645)
                     /locus_tag="PLB25_11590"
     CDS             complement(1860..2645)
                     /locus_tag="PLB25_11590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE42253.1"
                     /translation="MKKYLLSGIAMATLMVGLSPLALAQNIAVVNGKAVPKARVEALS
                     QQVIRSGRQITPEVEQQIKDEVIAREIFIQEAQKQGLDATEDFKTQMELARQTILIRD
                     LFTNYQKANTVTDEEVKAEYDRFAAANSGKEYKARHILVEKEDEAKAIIAQLKKGGKF
                     DEIAKKSSKDPGSGAKGGDLDWAPAGNYVAEFSQALTQLAKGKMTETPVKSQFGYHII
                     RLDDVRDAQLPPLEQVKPQVQQQLQQQKLVKYQNDLRSKAKVE"
     gene            complement(2707..2988)
                     /locus_tag="PLB25_11595"
     CDS             complement(2707..2988)
                     /locus_tag="PLB25_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HOE42254.1"
                     /translation="MSSTPTAQALQQRLQQALNPQALEVIDESAQHHGHAGANGSGFG
                     THFRVRITSHLFTGKTPVARHRLVYDALQDFMDHGLHALAIEAKVPAAS"
     gene            complement(2985..3533)
                     /locus_tag="PLB25_11600"
     CDS             complement(2985..3533)
                     /locus_tag="PLB25_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HOE42255.1"
                     /translation="MKILIDFFPILLFFGAYKYYDIYVGTAVLMVATLGQMTLIYAID
                     RHLQAMHKITLALILVFGTLTLVLHDERFIKWKPTVLYAAMAIGLAIAVWLLKKNFLK
                     MLLGSQLSLPEQVWMRLNMIWVAYCFFMASINGYVAAYYSTEDWVNFKLWGYAFPLVF
                     IVGQGFYISRYIAAEDTEKPTP"
     gene            complement(3530..3937)
                     /gene="msrB"
                     /locus_tag="PLB25_11605"
     CDS             complement(3530..3937)
                     /gene="msrB"
                     /locus_tag="PLB25_11605"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOE42256.1"
                     /translation="MPYPVQKTDAQWQALLKDKGAEPSAFQVTRQAATERPFSGKYET
                     HWADGSYHCMCCGNKLFAADSKFDAGCGWPSFSLALPGAITEITDRSHGMARVETVCS
                     QCGAHLGHVFDDGPTESGLRYCMNSASLNFTPT"
     gene            complement(3967..5508)
                     /locus_tag="PLB25_11610"
     CDS             complement(3967..5508)
                     /locus_tag="PLB25_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HOE42257.1"
                     /translation="MNAQLAPVELVKTGLVWDNTFYRLGKSFFTPLAPQPLGQPYWVG
                     RSLAVADLLGLEPDWLRSEALLQALSGNLPISGTQPLASVYSGHQFGQWAGQLGDGRA
                     ILLGETGGLEVQLKGSGLTPYSRMGDGRAVLRSSIREFLCSEAMHALGIPTSRALCVT
                     GSNDAVRRETLETAAVVTRVAPSFIRFGHFEHFSARNQLTELKTLADHVIDRFYPECR
                     TSTDFDGNAYAALLQRVTERTAVMVAHWQAAGFCHGVMNTDNMSILGLTIDYGPFQFL
                     DAFDPDHICNHSDRQGRYAFERQPDIAHWNLFALAQALLPLLPDQASALRALASFKPV
                     FNDKFHRLMCAKLGWPETAGTATGCDQDAVSWPQGDSQRMAARQALIDQILALLAQDR
                     VDYNIFWRRLSQARASGNPAPVRDLFLDRPALDAWMLLYNELSAHIEQGLQADLMLKT
                     NPKYVLRNHLGEVAIRAAGSGDYAGVADLLALLEKPFDEHPAFEAFADFPPDWASQIE
                     ISCSS"
     gene            5697..>6041
                     /locus_tag="PLB25_11615"
     CDS             5697..>6041
                     /locus_tag="PLB25_11615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HOE42258.1"
                     /translation="MTPQDYVQQKAAASGSSFYYAFLFLPAPKRAAITAFYAFCREVD
                     DVVDEATDAGVAATKLAWWQAEVAKSYAGQPSHPVMLALMPIAQQFGIAQHHLQAVID
                     GCQMDLTQTRYLD"
BASE COUNT         1284 a         1739 c         1721 g         1297 t
ORIGIN      
        1 aaagaaaaat cgcaccaacc cagattttcc gcaaccctgc cctctccgcc agcgccagca
       61 gcagcaaaag cagcaggaac aaaatcacat aggcaatgaa ccggcggtga atccgtcgaa
      121 ggtaaaggcc atatcctgga tcattgatcg gctcgatgcc atccgcacga ttggcaaacc
      181 aggcgtaaat agctaccaaa gcaatgaaaa ccagtacgct gccttgcgct gcaaaccaat
      241 aggccagagg ccaaccagcg atgacctgct gcagatcatg ggcaaaaaaa acaaccccga
      301 aggacgcaag aaaccaggcc agcaacaacc agagtcgcaa acgtgaaacg cttgcaactg
      361 gctggtgctc tggcggctcg aattcggggt tcatcaacag accgtgatga catccatgga
      421 aggccaactc aattgatgcc gactactgtt tggtatcgtc ataactcgca ttttttccca
      481 gccaaccgag tcaacggaag acactcccag gttttcaaag ggcacgacca cagcggtcgc
      541 agcaaacaaa gccgccatcg gaacaactcc agtacgcaaa acaccggctc aggcaaacag
      601 gcggtggctg gcgcctgcct tgacggcacg catttgttcg gcctgccact tgtacctgct
      661 gaaccaggac aacacctcaa agttgaccgg atcgatttga ttgtaggctt taagaagtct
      721 tacaagtcgc ccccacaatg cccgggcaaa ggggcaaacg gcgatgtgct gtgagttcaa
      781 ttcaacaagt tgccatgccc agctgccact ggctgcacaa gcggtttgca acaacgcatt
      841 gcgcttgtca gtgcggccga actgttcaag taattcttgc ttgaccctta agcacctggc
      901 cggatcaaaa caggaatggt tctttcgaat tgtgtgtcgc tcgcggcttc acctggcatg
      961 ggcggtccac tcccaagtga gcgttacgcc gttttcagag cccgcatgct ggtcactgcc
     1021 gtctcaccgt atgaacttga tgggccgcac atgccgactc ccgggtacca agcagccgct
     1081 tttttctgcc agttccttac acttgcccca tgaaagcaca acgcttcgaa gtgatcgatg
     1141 ccctgcgtgg cctggcagtg gtctggatga cgttttttca cttgggcttc gacttgaacc
     1201 attttggcta catccggcag aatttttatg ccgatccgct gtggacctgg cagcgcacgc
     1261 tgatcgtcag cctgtttctg ttttgtgccg ggctcggtca ggcagtggca ctggcgcagg
     1321 gccatggctg ggcacgattc tggcagcgct gggtgcaggt ggtggcttgt gcattgctgg
     1381 tcacgctggg gtcttggctg atgtaccccg gcagtttcat ttacttcggt gtcttgcacg
     1441 gcctggcgct gatgctgctg ctggcccgtc tgacctgcca ttgggggcgc tggctgtggt
     1501 ggctgggttg gctcgcgatt acatcagtat tgatagcacc ctacgccatg aactcatggg
     1561 ctacaagcca attgattgaa ttattcaacg cgccgatgct gaactggatc ggctggatta
     1621 cagaaaaacc ggtgactgaa gactacgtgc cgctgctgcc ctggctcggg ctggtgtggt
     1681 ggggcctggc taccggcaaa tggttgctgg acaccaagcc tggcgtgttg cgacaggctt
     1741 tggcacctgc gctcaaaccc ctggcctggc tgggccgctg gagcctgagc tattacatgc
     1801 tgcaccagcc ggtgctgatc ggcgggctga tgctggcgag ctggctggtc aaataaaaat
     1861 cactcgacct tggctttgct gcgcaagtca ttttggtact tgaccagttt ttgctgctgc
     1921 aattgttgtt gcacctgagg tttgacctgc tccagcggcg gcaactgcgc atcgcgcacg
     1981 tcatcgaggc ggatgatgtg gtagccaaac tgcgatttaa ccggggtctc ggtcatcttg
     2041 cccttggcca actgggtcaa tgcctgggag aactcagcca cgtaattgcc ggccggggcc
     2101 cagtcaaggt cgccaccctt ggcaccagag ccggggtctt tgctggattt tttggcaatt
     2161 tcgtcaaatt tgccaccttt tttgagttgc gcgatgatgg ccttggcctc gtcttctttt
     2221 tccaccagaa tgtgccgtgc cttgtattcc ttgccactgt tggcagcggc aaagcggtcg
     2281 tactctgcct tgacttcttc gtctgtgacg gtgttggctt tttggtaatt ggtgaacaga
     2341 tcgcggatca ggatggtctg gcgcgccagc tccatctggg tcttgaagtc ctcggtggca
     2401 tcaagacctt gcttttgggc ttcctgaatg aagatttcac gggcgatcac ttcatccttg
     2461 atctgttgtt cgacctcagg tgtgatttgg cggccagagc gaataacctg ctggctcaac
     2521 gcttcaacgc gggcttttgg caccgctttg ccattgacaa cggcgatgtt ctgcgccaac
     2581 gccaggggtg acaggccaac catcaaggtt gccatggcaa taccggacaa gagatatttc
     2641 ttcatgcgag tttcctgagt ttcaaataaa taaaggggaa aaggagtgtt gggttttttg
     2701 cccgcgttag cttgctgcgg gcactttggc ttcaatggcc agcgcgtgca ggccgtggtc
     2761 catgaaatct tgcagcgcat catacacaag ccgatgccgc gccaccgggg tcttgccggt
     2821 aaataaatgt gaagtgatgc gcactcgaaa atgggtgcca aagccactgc cattggcacc
     2881 tgcatggccg tggtgctggg cgctttcgtc aatcacctcc agcgcttgcg ggttgagcgc
     2941 ctgttgcagc cgctgctgca aggcctgggc ggtcggggtg ctgctcatgg tgtcggcttt
     3001 tccgtgtctt ctgctgcgat gtagcgtgaa atataaaagc cctgcccgac aatgaacacc
     3061 agcgggaagg catagcccca aagcttgaaa ttcacccagt cttcggtgct gtagtaggct
     3121 gccacatagc cgttgatgct ggccatgaaa aagcagtacg ccacccagat catgttcagc
     3181 cgcatccaga cctgctcggg caaactcaac tggctgccaa gcagcatctt caaaaagttt
     3241 ttcttcagca gccacaccgc aatcgccagg ccaatcgcca ttgcggcgta cagcacggtc
     3301 ggtttccatt tgatgaagcg ttcgtcatgc agcaccagcg tcagggtgcc aaaaaccaga
     3361 atcagcgcca atgtgatctt gtgcatggcc tgcaagtgcc ggtcgatcgc gtaaatgagc
     3421 gtcatttgcc ccaacgtggc gaccatcagc acggcggtgc caacgtagat gtcgtaatac
     3481 ttgtaggcac caaaaaacag caggatggga aaaaaatcga tcagtatctt catgttgggg
     3541 tgaagtttaa cgaagccgag ttcatgcaat agcgcaggcc ggactcggtc gggccgtcgt
     3601 caaacacatg acccagatgc gcgccacatt gactgcagac ggtttccacc cgtgccatgc
     3661 catggctgcg gtcagtgatt tcggtgatgg cacctggcag cgccaatgag aaactgggcc
     3721 agccacaacc agcgtcgaac ttgctgtcgg cggcaaacag cttgttgcca cagcacatgc
     3781 agtggtaact gccgtcggcc cagtgggttt catacttgcc gctgaacgga cgctcggtgg
     3841 cggcctggcg ggtcacctga aaggctgacg gctcagcgcc cttgtctttg agcagggctt
     3901 gccattgggc atcggtcttc tgtacggggt agggcatggg tggtccttgg gggttgtatc
     3961 acaacatcac gaactgcaac tgatttcgat ctggctggcc cagtcgggtg gaaagtcggc
     4021 aaaggcttca aaggccgggt gttcatcaaa cggcttttcc agcagcgcca acaggtctgc
     4081 aacaccggca tagtcaccgc ttccggcggc ccggatggcc acttcgccca gatggttgcg
     4141 aagcacatac ttcgggttgg tcttgagcat caaatcggcc tgtagccctt gttctatatg
     4201 cgctgacagc tcattatata atagcatcca ggcatccagg gcggggcgat ccagaaacag
     4261 gtcccgcacc ggtgccgggt tgccgctggc gcgtgcctgg ctcaggcgac gccaaaagat
     4321 gttgtagtcc acccggtctt gtgccagcag cgccagtatt tggtcgatca gcgcttgccg
     4381 tgccgccatt cgctggctgt cgccctgtgg ccacgacacc gcgtcctggt cgcagccagt
     4441 agccgtacca gcggtctcag gccagcctag cttggcacac atcaaccggt gaaatttgtc
     4501 attgaacacc ggcttgaaag atgccagtgc ccgcagcgcc gatgcctggt caggcagcag
     4561 cggcagcagc gcctgcgcca gcgcgaacag attccagtgg gcaatgtctg gctggcgctc
     4621 aaaagcatag cggccctggc ggtctgaatg gttgcaaatg tgatccgggt caaacgcatc
     4681 aagaaactga aacgggccgt agtcgatggt cagacccaga atgctcatgt tgtcggtgtt
     4741 catgacccca tggcaaaaac ccgccgcctg ccaatgtgcc accatcaccg ccgtgcgttc
     4801 ggtgacccgc tgcagcaggg ccgcgtaggc attgccatcg aaatcggtgc tggttcggca
     4861 ttcggggtaa aagcgatcaa tcacatggtc agccagcgtt ttgagctcgg tgagctggtt
     4921 tctggcagaa aagtgctcaa aatgaccaaa gcggataaag cttggtgcca cgcgggtcac
     4981 caccgcagcg gtttccagcg tctcgcgccg caccgcgtcg ttggagccgg tcacgcacaa
     5041 tgcgcgcgag gtcggaatgc ccagggcatg catcgcctcg ctgcacaaaa attcgcggat
     5101 gctgctgcgc aataccgccc gaccgtcgcc catgcgtgag tagggtgtca ggccagagcc
     5161 cttgagctgc acttcaagcc cgccggtttc acccagcaaa atggcgcgcc cgtcaccgag
     5221 ttgcccggcc cattggccaa actgatgacc actgtagacg ctggccagcg gctgcgtacc
     5281 tgaaatcggc aggttgcccg acagtgcctg cagcagcgcc tcggatctca gccagtcagg
     5341 ctcaagcccg agcaaatcag caaccgccag gctgcgccca acccagtagg gctgaccaag
     5401 tggctgcggt gccagcgggg tgaaaaaact tttgcccagg cggtaaaagg tgttgtccca
     5461 gaccagcccg gttttgacca gttcaacagg ggcaagttgt gcgttcatca ccacattgtc
     5521 ccgaagcagc gacaaaccct gtgcccacag ggacaatcgg gcctgcgctg gcaattggcc
     5581 agcacgacga agctgcaggc gcgcggtaca ccactgtcgg ggtggcaaat ggccgacaaa
     5641 aatgcacctg cgtcacatca cctgtttaaa ctgagtcaaa acagccaacc caagccatga
     5701 ccccgcaaga ctacgtccag caaaaagccg ccgcatcagg cagcagtttc tattacgcct
     5761 ttttgtttct gcccgcaccc aagcgtgccg ccatcacggc gttttatgcg ttttgccgtg
     5821 aagtcgatga cgtggttgat gaggccaccg atgccggtgt ggccgccacc aaactggcct
     5881 ggtggcaggc cgaggtggcc aagtcctacg ccggccagcc gagccacccg gtgatgctgg
     5941 ccttgatgcc gattgcccag caattcggca ttgcgcagca ccatctgcag gccgtcatcg
     6001 acggttgcca gatggacctg acgcaaaccc gttaccttga t
//
LOCUS       DAOSLL010000251         6035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11846_length_6035_cov_4.961706, whole genome shotgun sequence.
ACCESSION   DAOSLL010000251 DAOSLL010000000
VERSION     DAOSLL010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11846_length_6035_cov_4.961706"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="PLB25_11620"
     CDS             complement(<1..744)
                     /locus_tag="PLB25_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE42259.1"
                     /translation="MTTPSRTTPLAAPPPRRKLPIGIQTFSKLREEGCYYVDKTALAL
                     HLIDSGSYYFLSRPRRFGKSLFLDTLKELFEGNAALFHGLAAENSWDWGKQFPVIRIS
                     FGDGVLRSRTELDTRIHEILLDNAQRLGVVCTHESIGARFEQLIARVHQKVGQRAVVL
                     IDEYDKPVLDNIDNSPVAIEMREGLKNLYSVLKGSDEHLKFAFLTGVSKFSKVSLFSG
                     LNHLTDITLDPRYSALCGYTDADLDTVFAP"
     gene            868..3264
                     /locus_tag="PLB25_11625"
     CDS             868..3264
                     /locus_tag="PLB25_11625"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE42260.1"
                     /translation="MNLRVFQWQSLKTRLTLLTVGIFVLGIWLLAFYASRILREDLQQ
                     MLGQQQFAAVSVMALEINDRLSDRLQAVESIAKQVTPAVLADAVALQKLLEQRPLLQL
                     LFNGGVFVTGANGTAMADVPRSAGRIGTNYLDRENVAVTLKEGKTVIGRPAMGKKLRA
                     PIFSISAPIRDGKGQVIGAMVATINLGQPSFLNQIMDGRYGQTGGYLLIAPQHKLIVT
                     GSDKSRAMQPVPDPGRNAMHDRYMLGYEGFGLAVSSRGVLELSAAKVIAATGWFVVAT
                     LPAQEAFAPVNHMLQRLLLGAMVFTVLAGALTWWLIQHLLQGQLSPLLAASRSLAAQV
                     DSGQPVQALTVQRQDEIGELIGGFNRLLAATAKREQALAESEAKFRAVVDLSPDAILL
                     HRKAKLTFANPAAIAMFGASTEQDLIGKPVLELIHPDYHQVVLDRIRNTHQTGRPEPR
                     IEERFVKLDGTCIDVEASGRLIVLEGEPFVFVTARDVTERNQDQARLRLAASVFAHAG
                     EGIMITGANANIMEVNEAFTRITGYSRDEVLGKNPHILNSGRHDADYFAAMWRSLTEQ
                     GYWSDEIWNKRKNGELFAERLTVSTVRDASGSVQHYLALFSDITEHKVMEDRVRQMAY
                     FDALTGLPNRRLLGDRLSQAMAASKRNGLYGALMFLDLDNFKPVNDTLGHDVGDLLLI
                     EVARRLTDGVREADSVARLGGDEFIVMLTELGSDQAEAAGQAHAVAEKIRDSLAAPYQ
                     LMVLHADGDSTTVEHRCSASIGVVMFGNQQVSQTDMLVRADAAMYQAKQAGRNRVRFG
                     "
     gene            3396..>6035
                     /locus_tag="PLB25_11630"
     CDS             3396..>6035
                     /locus_tag="PLB25_11630"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE42261.1"
                     /translation="MNSKVSLWRSLQARATVFTLAVFVLGIWALSLYASGLLQADLER
                     VLGQQQLSTASVMAHEINDRLRDRLQALETIAKEVDADLMGQPGALQTRLEQRPLLQL
                     LFNGGAWAAATDGVAIADVPGWAQRIGTSYRDVDFIAAALKDGKASISRPVIGKKRMM
                     PIFAITVPIRTKQGQVIGVLTGVTDLGKPNFLDLIAQSDYGKTGGYLLIAAEHRMVIS
                     ATDKSRIMEPLPTVGSNASIDRFANGYEGSAVLTNPRGVEVLVSGKGIPVAGWYVLVS
                     LPTAEAFAPVRDLQQRLLWATLLLTLLTGALTWWVLKRQLVPLVATADALAALADAQQ
                     MAQPLSATRPDEIAYLVDGFNRILARWTQREAALTDSQQNLAITLNSIGDAVIATDAA
                     GLITRMNPTAERLTAWPLADALGRPLTEVFSIVNASTREPVAAPVQRVIECGEVVGLA
                     NHTMLLAKDGREYQIADSAAPIRNAAGDIVGVVLVFSDVTEKYQVEQALLETEWKFRA
                     LFEKGPIGVAYHEMIYDDQGRPKDYFFIDANATYQELTGVNPLGKTVTQAFPGIENDP
                     FDWIGTYGRVARTGEPVRFEQFLPSNGCWYDCAAYQFKPDGFVVAFLNITERKKAEQD
                     ARESHRRLSLALDAAAIGEWEIDLATQSWHRSLRHGAIFGQSEPLPLWNLATFFDHVH
                     PDDLGRVKGSVQQAQALAQDWRDEFRIVRDDQAVRWVWATGHVLMNAQGLPEKAVGMV
                     SDITERKQAEEALRIAATAFESQQGMVVTDAQRMILRVNQAAADITGYSADEAVGQNP
                     RMLKSGRHDSGFYDAMTQALEQDGIWAGEIWNRRKNGEIYPEWLSISAVKDDAGLTSH
                     FVGIFTDISERINAQAQIDTLAFY"
BASE COUNT         1197 a         1759 c         1889 g         1190 t
ORIGIN      
        1 gggcgcaaac accgtgtcaa ggtcagcgtc ggtgtagccg cacagggcgc tgtagcgcgg
       61 atcgagggtg atgtctgtca ggtggttcag gccagaaaac aaactcactt tgctgaactt
      121 gctgaccccg gtgagaaaag caaacttcaa atgctcatcc gagcccttca acaccgagta
      181 caggtttttg agcccttcgc gcatctcgat cgccaccggg ctgttgtcga tgttgtccag
      241 aactggcttg tcgtattcgt caatcagcac caccgcgcgc tggccgacct tttggtgcac
      301 tcgggcgatc agttgttcaa agcgtgcgcc gatgctttca tgggtacaga ccacgcccag
      361 gcgctgcgcg ttgtcgagca ggatctcgtg aatgcgggtg tcaagctcgg tccggctgcg
      421 cagcacgccg tcgccaaagc tgatgcgaat cacggggaat tgtttgcccc agtcccagga
      481 gttctcagcg gccaggccgt ggaacagcgc cgcgttgccc tcaaacagct ctttgagggt
      541 gtccaaaaac aggcttttgc caaagcggcg cgggcgactc aagaagtagt agctgccact
      601 gtcgatcagg tgcagggcca gcgcggtctt atcgacgtag tagcagcctt cttcgcgtag
      661 tttggaaaaa gtctgaatcc cgatgggcag cttgcgccgc ggcggcggcg cagccagcgg
      721 ggtggtgcgt gacggggtgg tcatcaaccc attttagggt gatgcccacc gtcgccacgc
      781 tgccggttgc ttgccatggt ggtcattgca gcaagcgtgg cgggggtacc atgtgcttct
      841 ggcggcttga tgtccaagga aatcggcatg aacttacgcg tctttcaatg gcaatcgctc
      901 aagacccgtc tcaccctgtt gacggtgggc atatttgtgt tgggcatctg gctgctcgcg
      961 ttctatgcca gccgcatcct gcgcgaagac ttgcagcaaa tgttgggtca gcagcagttt
     1021 gctgcagtgt cggtgatggc cctggaaatc aatgaccgcc tgagtgaccg tctgcaggcg
     1081 gtggaatcga ttgccaaaca agtgaccccg gcggtactgg ctgatgccgt cgccctccaa
     1141 aagttgctgg agcagcgccc gctgctgcaa ctgctgttca acggcggcgt cttcgttacc
     1201 ggggccaatg gcacggcgat ggctgatgtg ccacgttcgg ccggccgcat tggcaccaac
     1261 tacctggacc gggaaaatgt tgccgtgacg ctcaaggagg gtaaaacggt gattggccga
     1321 cccgccatgg gcaagaagct gcgagcaccg atctttagca tcagcgcccc gattcgtgat
     1381 ggcaaaggcc aggtgatcgg ggccatggtg gcgacgatca acctggggca accgagtttt
     1441 ctcaaccaga ttatggacgg ccgctacggc cagaccggcg gctatctgct gatcgcgcca
     1501 cagcacaagc tgattgtcac cggctccgac aagagccgcg ccatgcagcc ggtgccagac
     1561 cccgggcgca acgcgatgca cgaccgctac atgctgggtt atgagggctt tggcctggcc
     1621 gtcagttcgc gcggcgtact tgagttgtcg gctgccaagg tgattgcggc aactggctgg
     1681 ttcgtcgtgg ccacactgcc agcgcaggaa gcctttgcgc cggtcaacca tatgctgcag
     1741 cgcttgctgc tcggtgccat ggtctttacc gtgctggccg gtgcgctgac ctggtggctg
     1801 attcagcacc tgttgcaagg gcagttgtca ccgctgcttg ccgccagccg ctcgctggcc
     1861 gcccaggtcg acagtggcca gccggtgcag gcgttgacag tgcagcgcca ggacgagatc
     1921 ggtgagttga tcggcggctt caaccgcctg ctggcagcga cggctaagcg cgagcaggct
     1981 ttggccgaga gcgaggcaaa atttcgcgca gtggttgatc tgtcacccga cgccattttg
     2041 ctgcaccgca aggccaagct gacgtttgcc aacccggccg ccattgcgat gtttggtgcc
     2101 agcaccgaac aagacctgat cggcaagccg gtgcttgagc tgattcatcc ggactatcac
     2161 caagtcgtgc tggatcggat caggaacacc caccagaccg gtcggccgga gccgaggata
     2221 gaagagcgtt ttgtcaagct cgatggcacc tgtattgacg tggaagcttc gggccgtctg
     2281 attgtgctgg agggtgaacc ctttgttttt gtcacggcgc gcgatgtcac cgaacgcaac
     2341 caggaccagg cccggctacg ccttgccgcg agtgtcttcg cccatgctgg cgagggcatc
     2401 atgatcaccg gggcaaatgc caacatcatg gaggtgaatg aggccttcac ccgcatcacc
     2461 ggctacagcc gcgacgaggt gctggggaaa aacccgcaca tcctgaattc tggccgccat
     2521 gacgcggact actttgccgc catgtggcgc agcctgaccg aacagggcta ctggagcgat
     2581 gaaatctgga acaagcgcaa aaacggcgaa ctgtttgccg agcggctaac cgtcagcacc
     2641 gtgcgtgatg ccagcggcag cgtgcaacat tacctggcct tgttctctga catcaccgag
     2701 cacaaggtga tggaagaccg ggttcgccaa atggcctact ttgatgccct aaccggcctg
     2761 cccaaccgcc gcctgcttgg tgatcgcttg agccaggcca tggccgccag caaacgcaac
     2821 gggctttatg gggccttgat gtttctggac ctggacaact tcaagccggt caacgacacg
     2881 ctgggccacg atgtgggtga cttgctgctg atcgaggtgg ctcggcgctt gaccgacggc
     2941 gtgcgcgaag ccgacagcgt ggcccgtctc ggcggcgatg agttcatcgt gatgctcacc
     3001 gaacttgggt ctgaccaggc cgaggccgcc ggacaggccc acgccgtggc tgaaaaaatt
     3061 cgcgacagtc tggcggcacc gtaccagttg atggtgctgc atgcggacgg cgacagcacc
     3121 acggtagagc atcggtgcag cgccagcatt ggcgtggtga tgtttggcaa ccagcaagtc
     3181 agccagaccg acatgctggt acgggctgat gcggccatgt accaggccaa gcaagcagga
     3241 cgcaataggg ttcgattcgg ttgactgtgt gaccgctgtt gcagcgccag atggtgcgcc
     3301 gccccggcag tcaaatagtg acaaaaaaat gctgccagcc catttgaata ctgcgtgagt
     3361 accgataaag ttgatatgtc tgttggaaca aacctatgaa ctcgaaagtc tcattgtggc
     3421 ggtcgctcca agcccgtgcc accgtcttta cgctggcggt ttttgtgctc ggtatctggg
     3481 ccctgtcgtt gtatgccagc ggcttgctgc aagccgatct ggaacgcgtg ctgggtcagc
     3541 agcagttgtc gaccgcctcg gtgatggcgc acgaaatcaa tgaccgcctg cgtgaccgcc
     3601 tgcaggcgct ggaaaccatt gccaaagaag tcgatgccga cctgatgggc cagccaggtg
     3661 ccttgcaaac ccggctggag cagcgcccgc tgctgcaact gctgttcaac ggcggcgcct
     3721 gggccgccgc aacggatggc gttgccatag ccgacgtgcc gggctgggca cagcgcattg
     3781 gcacctctta ccgggatgtg gatttcatcg ccgcagccct caaggacggc aaggcatcga
     3841 tcagccgacc ggtcattggc aaaaagcgca tgatgcccat ctttgccatc acagtgccga
     3901 ttcgcacgaa gcaaggccag gtcattggtg ttctgactgg ggtaaccgat cttggcaagc
     3961 cgaattttct cgatctgatt gcgcagagcg actacggcaa gaccggcggc tatctgctca
     4021 ttgccgcgga gcacaggatg gtgatctcgg ccaccgacaa aagccgcatc atggagccgc
     4081 tgcccacagt gggcagcaat gcgtcgattg accggttcgc caacggctat gaagggtcgg
     4141 ccgttttgac caatccacgg ggtgtggaag tgctggtttc gggcaaaggc attcctgttg
     4201 ccgggtggta tgtgctggtc tcgctgccca cggcagaagc ctttgcgccg gtgcgcgacc
     4261 tgcagcagcg tttgctctgg gccacattgc ttttgacatt gttgaccggc gcattgacct
     4321 ggtgggtctt gaaacgccag cttgtccccc tggtcgccac ggccgatgcg ctggcggcgc
     4381 tggctgatgc acagcagatg gcacagcccc tgagtgccac ccgaccagac gaaatagcct
     4441 atctggtcga tggctttaac cgcattctgg cccgatggac gcaacgcgag gcggcgctga
     4501 ccgacagcca gcaaaacctg gccatcacgc tcaactcgat tggcgacgcg gtgattgcca
     4561 ccgatgcggc tggcctgatt acccgcatga acccgaccgc cgagcgactc accgcctggc
     4621 cgctggccga cgcactgggc cgaccgctga ccgaggtgtt tagcattgtg aatgccagca
     4681 cgcgcgagcc ggttgccgcc ccggtgcaac gggtgatcga atgtggcgag gtggtggggc
     4741 tggccaacca caccatgttg ttggccaaag acggccggga gtaccagatt gccgacagcg
     4801 ccgccccgat ccgcaacgcg gcaggcgata tcgtcggcgt ggtgctggtg ttcagtgatg
     4861 tcaccgaaaa ataccaggtc gagcaggctt tgctggagac tgagtggaag ttccgcgccc
     4921 tgtttgaaaa aggtccgatc ggggtcgcct accatgaaat gatttacgac gaccagggtc
     4981 ggcccaaaga ttattttttc atcgatgcca atgccactta tcaggaactg accggggtca
     5041 acccattggg caaaaccgtc acccaggctt ttcccggcat cgaaaatgac ccgtttgact
     5101 ggattggcac ttatggccgt gtggcgcgca ctggcgagcc ggtccgcttt gagcagtttc
     5161 tgccgtccaa tggctgttgg tacgactgtg ccgcctacca gttcaagcca gacggctttg
     5221 tggtggcgtt tttgaacatc accgagcgca aaaaggccga acaagatgcc cgggaatcgc
     5281 atcgccggct tagcctggca cttgacgctg cggccattgg cgaatgggag atcgacctgg
     5341 ccacccaaag ctggcaccgc tcactccggc acggcgcgat ttttggccag agcgagccac
     5401 tgccgctctg gaacctggcg accttttttg atcacgtgca cccggatgat ctgggccggg
     5461 tgaaaggctc ggtgcagcag gcgcaggcgt tggcccaaga ctggcgggac gaatttcgca
     5521 ttgttcgtga cgaccaggcg gtgcgctggg tttgggccac cggccacgtt ttgatgaacg
     5581 cacagggcct gcctgaaaaa gcggttggca tggtctctga catcaccgag cgcaaacagg
     5641 ctgaagaagc gctgcgcatt gccgccaccg cgtttgagtc gcagcaaggc atggtcgtca
     5701 ccgatgcgca acgcatgatt ctgcgggtca atcaggccgc tgcagacatt accggctaca
     5761 gcgccgacga agcggtgggg cagaacccgc gaatgctgaa atcaggtcgc cacgacagcg
     5821 gcttctacga tgccatgaca caagcgctgg agcaagatgg catctgggcc ggtgaaatct
     5881 ggaaccggcg caagaatggc gagatttatc ccgaatggct cagcatcagc gcggtgaaag
     5941 acgatgccgg cctgaccagc cattttgtcg gcatctttac cgacatcagc gagcgcatca
     6001 acgcgcaggc gcaaattgac acgctggctt tttat
//
LOCUS       DAOSLL010000252         6007 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11917_length_6007_cov_5.323253, whole genome shotgun sequence.
ACCESSION   DAOSLL010000252 DAOSLL010000000
VERSION     DAOSLL010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6007)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11917_length_6007_cov_5.323253"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="PLB25_11635"
     CDS             <1..379
                     /locus_tag="PLB25_11635"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HOE42262.1"
                     /translation="VDYELLKAVVATESAFNAKAVSPKGAVGLMQIMPTTAQRYGVLA
                     DQGATVSAKLTDPELNIQTGTRYLADLLRLFGGQTELALAAYNAGEGAVARAGNRIPN
                     YKETQSYVTKVMSMYRLLQTRNG"
     gene            complement(388..621)
                     /locus_tag="PLB25_11640"
     CDS             complement(388..621)
                     /locus_tag="PLB25_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42263.1"
                     /translation="MTTTLDAINPFSLMMEPERVLHTMERSQQLRNLRRHKLRPLDKP
                     LIPYTSAALASRAAFDAMIDAQELDEQDDYLLN"
     gene            complement(820..2208)
                     /gene="dbpA"
                     /locus_tag="PLB25_11645"
     CDS             complement(820..2208)
                     /gene="dbpA"
                     /locus_tag="PLB25_11645"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010111493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HOE42264.1"
                     /translation="MTNNDPLAFNTLALNPATLDNLAQLGYLVMTPIQAMSLPQALAG
                     IDLIAQAETGSGKTAAFGLSLLERLNPRRFAVQALVLCPTRELADQVTVEIRRLARAM
                     DNIKVVALCGGVALRGQRASLENGAHIVVGTPGRVMDHLARGYLSLEALNTLVLDEAD
                     RMLDMGFFDDIATVTRQCPPERQTLLFSATYPPGIEKLARQFMRNPVQIQVKPASTQS
                     LIEQRFYEVTRPTRFEAVARLLNHFRPVSTLAFCNTKQQCKELVVYLQQQGFSALALY
                     GELEQRERDQVLAQFANRSSSVLVATDVASRGIDIKELSAVINVDITPDPEVHVHRIG
                     RTGRAGEAGLALSLAALYEMGAVGKIEQYQKLESVWHTLDELQATGSGPLLPPMVTLQ
                     ILGGRKEKIRPGDVLGALTAEAGFSREQIGKINVTEFTTFVAVQRDIALQAMQKLNVG
                     KVKGKNVKVRLL"
     gene            2375..3529
                     /locus_tag="PLB25_11650"
     CDS             2375..3529
                     /locus_tag="PLB25_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HOE42265.1"
                     /translation="MPTLHISSQFDAGAIEVVSLADPQDIRLNIRRDNASEFAQWFYF
                     CLHGAAGLPVCLNFLNAGQSAYPKGWEAYRVVASDDRQNWFRLDTEFDGQVMRVAFTP
                     TGNSVYLAYFEPYSHERHLDLLGSVALSDHVTTERLGSTLDGRDMTLLQITNAQSTTP
                     LAQRRKIWLIARQHPGETMAEWFVEGFLQRLLDAADPVARVLLDQCVFYLLPNMNPDG
                     AVRGNLRTNAVGANLNREWAAPSLEKSPEVYWVRQKMLEVGVDLFLDAHGDEGLPYNF
                     VAGTEDTPGYNPRIAALEDLFKTSWQAACPDFQTTHGYPRGKHGEANMTLATNWVGQQ
                     FGCLAFTIEMPFKDNADLPDARVGWNGERSKRLGASLLQPLLTLLPALRK"
     gene            complement(3817..5307)
                     /locus_tag="PLB25_11655"
     CDS             complement(3817..5307)
                     /locus_tag="PLB25_11655"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HOE42266.1"
                     /translation="MNKPFDTGLMWFRRDLRVSDNAALHLALKSCRQLHCVFVFDSDI
                     LDGLPRADRRVAFIRESLVQLDQALRQLSGLAGSGLIVRHALASSEIARLAAQLKAQA
                     VFAARDYEPLALARDVQVKSALAQAGIAFQTCKDQVIFEGRDILTQGGTPYSVFTPYK
                     NNWLSKLGAEHLRDHDCKPLACALAERPPAYQTDVPSLKDIGFEASPASGLKIEAGLQ
                     GGSALFEDFFGRIDDYHSSRDFPAIKGPSYLGVHLRFGTLPIRQLVATAWQRQLAGSQ
                     GAAVWLGELIWRDFYFSILANFPHVAQSAFKSEYDAIKWESGAAADALFAAWCQGRTG
                     YPLVDAAMAQINQSGYMHNRLRMVAGSFLVKDLGLDWRWGERYFAMQLNDFDLSANNG
                     GWQWVASSGCDAQPYFRIFNPVSQSEKFDPKGKFIRRYLPQLARLSDKSIHAPWTAKP
                     LELQAAGITLGQDYPYPLVDHAAARQRTLQRYGVVKKANPTPADKV"
     gene            5329..5928
                     /locus_tag="PLB25_11660"
     CDS             5329..5928
                     /locus_tag="PLB25_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HOE42267.1"
                     /translation="MTADSAPINLTNHFLIAMPGLQDDLFGHSVVYLCEHSQRGALGL
                     VINKPCDISLKGLFDKVELPLTRQDLTEAQVFYGGPVQTERGFVLHEATFADADKPSD
                     PVYSSTMSIPGGLEMTTSKDVLEAISTGGGPRKLLVSLGYSAWGEGQLESEIGENSWL
                     TVLADRRLIFDTPVAERYAQALQLLGLQAWMLSPDAGHA"
BASE COUNT         1175 a         1798 c         1802 g         1232 t
ORIGIN      
        1 cgtggattac gagctgctca aggcggtggt ggcgaccgag tcagccttca atgccaaggc
       61 ggtgtcaccc aaaggcgcag tgggcctgat gcagatcatg ccgaccacag cgcaacgcta
      121 tggcgtgttg gctgaccagg gcgcaacagt atcagccaaa ctgaccgacc cggagctcaa
      181 tattcaaacc ggcacccgct atctggcgga cctgctgcgc ttgtttgggg gtcaaaccga
      241 gttggccttg gctgcttaca acgccggtga aggcgcggtt gcacgcgctg gcaaccgcat
      301 tcccaattac aaagaaacac agtcttacgt caccaaggtc atgtcgatgt accgtttgct
      361 gcaaacccgc aacggctgaa ggccggttca attgagcaag tagtcgtctt gctcgtcaag
      421 ttcctgcgcg tcaatcatgg catcgaaagc ggcccggctg gccaacgcag ccgaggtgta
      481 cggaatcagt ggcttgtcga gcggacgcag cttgtggcgg cgcaggttgc gcagttgttg
      541 cgagcgttcc atggtgtgca agacccgttc cggttccatc atcaaactga acgggttgat
      601 ggcatcaagg gtagttgtca tggtggactc ctgctgtgtc aaacacgttt tgcgaactgc
      661 aaaaccttga aatgaactgt atccctgcac ctgcaaaagc taaagtcggc attttgctcg
      721 atgctgcgtc agaacttgcc tgacaaggcg taggcctcga acgattcagt gcattgtggt
      781 caggccagca tcaccccacc agcctgtcgg ctttttgctt cacagcagcc gcactttgac
      841 atttttgccc ttgaccttgc ccacattgag tttttgcatg gcttgcagcg caatgtcacg
      901 ctgcaccgca acgaaggtgg tgaactcggt gacgttaatt ttgccaatct gctcacggct
      961 aaagccggcc tctgcggtga gcgcgccgag cacatcacca gggcggatct tttccttgcg
     1021 acctcccaaa atttgtagcg taaccatggg cggcagcaag ggcccggagc cagtagcttg
     1081 cagttcatca agagtgtgcc agaccgattc gagcttctgg tattgctcga tcttgccgac
     1141 cgcacccatc tcgtacaggg cggccaggct cagcgccagg cccgcctcgc cggcgcggcc
     1201 ggtgcggcca atgcggtgca catgcacctc ggggtcgggg gtgatatcga cgttgatgac
     1261 ggctgacaat tccttgatgt cgatgccgcg cgaggcgacg tcggtggcca ccagcaccga
     1321 gctgctgcgg ttggcaaatt gggccagcac ctggtcacgc tcgcgctgct ccagctcgcc
     1381 ataaagcgcc agtgcgctaa agccctgctg ttgcaaatac accaccaatt ccttgcattg
     1441 ctgcttggtg ttgcaaaacg ccaacgtgct gaccgggcga aagtggttca gcagcctggc
     1501 aactgcttca aaccgggtgg gccgggtaac ttcgtaaaag cgctgctcga tcaggctttg
     1561 ggtgctggcg ggcttcacct gaatctgcac cgggtttcgc atgaactgcc gggccagctt
     1621 ttcgatgcca ggcgggtagg tggctgaaaa cagcagcgtt tgccgctcgg gcgggcactg
     1681 tcgggtgacg gtggcaatgt catcaaaaaa gcccatgtcg agcatgcgat cggcttcgtc
     1741 aagcaccagg gtgttgaggg cttccaggct gaggtagccg cgcgccagat ggtccatgac
     1801 gcgcccgggc gtgccaacca cgatgtgtgc gccgttctca agtgaggcgc gctggccgcg
     1861 cagcgccacg ccaccgcaca gggccaccac cttgatgttg tccatggcgc gcgccaggcg
     1921 gcggatttcc acggttacct ggtcggccag ctcgcgagtt gggcacagca ccagcgcctg
     1981 cacggcaaaa cggcgcgggt tgagccgctc cagcaggctc aggccaaagg cggccgtctt
     2041 gccgctgccg gtctcggcct gggcgatcag gtcgatgccg gccagtgcct gcggcaggct
     2101 catggcctga atcggggtca tcaccaggta gccaagttgc gccaggttgt ccagcgtcgc
     2161 cgggttcagc gccaaggtgt tgaaggccag ggggtcattg tttgtcatgg cctgattatc
     2221 ggcaggcctg ccggttggtc tgcaagctgg ccggtgcatc gaaaaaagct atttttatga
     2281 tagcttgcag cgcttatacc ataagggcta gcgcatgatt tagcgtacaa atcaggtgcc
     2341 gggtcctaaa atcgggctcc catcaggaaa gttcatgcca acacttcata tttccagtca
     2401 gttcgatgcc ggtgccatcg aggtcgtcag cctggccgac ccacaagaca ttcggctcaa
     2461 cattcgccgc gacaacgcca gtgaatttgc ccagtggttt tacttttgcc tgcatggcgc
     2521 agcgggtctg ccggtgtgcc tgaacttttt gaatgccggg caaagcgctt acccgaaagg
     2581 ctgggaagct taccgggtgg tggccagcga tgaccgccag aactggtttc ggctcgatac
     2641 cgaattcgac ggacaggtga tgcgtgtggc cttcacgccg acgggcaaca gcgtctatct
     2701 ggcctatttt gagccctatt cccatgagcg gcatctggat ttgctgggct cggttgccct
     2761 ctcagatcac gtcacgaccg agcgtttggg cagcacgctc gatggccgcg acatgacgct
     2821 gctgcaaatc accaacgctc aaagcaccac gccgctggcg caaaggcgaa aaatctggct
     2881 gatcgcccgt cagcaccctg gcgaaaccat ggccgaatgg tttgtcgagg gctttttgca
     2941 gcgcctgctt gatgcggccg acccggtcgc ccgtgttttg ctcgaccaat gcgtgtttta
     3001 cctgttgccc aacatgaacc cggatggtgc cgtgcgcggc aacctgcgca ccaacgcggt
     3061 cggtgccaac ctgaaccgcg agtgggccgc accttcgctg gaaaaatcac ccgaggtgta
     3121 ctgggtgcgc caaaagatgc tggaagtggg cgtggacctg tttttggatg cccatggtga
     3181 cgaaggcctg ccctacaact ttgtcgcggg caccgaggac acgccgggct acaacccgcg
     3241 cattgccgcg ctggaagacc ttttcaaaac cagttggcaa gccgcttgcc cggattttca
     3301 aaccacccac ggctacccgc gcggcaaaca tggcgaggcc aacatgacgc tggccaccaa
     3361 ctgggttggc cagcagtttg gctgcctggc ctttaccatt gaaatgccgt tcaaggacaa
     3421 cgctgacctg cccgacgcac gcgtcggctg gaacggcgag cgctccaagc ggctcggagc
     3481 cagcctgctg cagccgctgc tcacactcct gccagcattg cgaaaatagt gcctgatgct
     3541 gctgcggccc ttgtctgata agcgctgaat gctatctttt gtacagtaaa ttgagagacc
     3601 tgaaaagatc accgcaggcg ttgtcggcgg gtggcgggtc agccttcttg accacaccat
     3661 agcgctgcag cctgcgttgc cgtgctgccg caaggtccac cagcgggtat cggcagtcct
     3721 gccccagcgt gatggctggt atggccgcag cccttgcctg ataagcgctg catgctatat
     3781 ttttaatagt aaattgagag gtctgaacag attgcctcag accttgtcgg cgggtgtcgg
     3841 gttagccttc ttgaccacac catagcgctg cagcgtgcgt tgccgtgctg ctgcatggtc
     3901 caccagcggg tacgggtagt cctgccccag cgtgatgcct gctgcctgca attcgagcgg
     3961 tttggcggtc cagggcgcgt ggatcgactt gtccgacaac ctggccagtt gcggcaggta
     4021 gcgccggatg aatttgccct ttgggtcaaa tttttcgctt tgactgaccg ggttgaagat
     4081 tctgaaatag ggttgcgcgt cacagccact cgatgccacc cattgccaac cgccattatt
     4141 ggcggacaaa tcaaagtcgt tgagctgcat ggcgaagtaa cgttcgcccc agcgccagtc
     4201 cagcccaagg tctttcacca gaaaactgcc cgccaccatg cgcagccggt tgtgcatgta
     4261 gccgctctgg ttgatctggg ccatggcggc atccaccagc gggtagccgg tgcggccctg
     4321 gcaccaggcg gcaaaaagcg catcggccgc ggcaccgctt tcccatttga tcgcgtcgta
     4381 ctcggacttg aaggccgatt gcgccacatg cggaaaattc gccagaatcg agaagtaaaa
     4441 gtctcgccag atcagctcac cgagccagac tgcggcaccc tggctgccag ccaactggcg
     4501 ctgccaggca gtggcgacca gttgccggat cggcagcgtg ccaaagcgca gatgcacccc
     4561 gaggtaactc ggccccttga tggccggaaa atcgcgactg ctgtggtagt cgtcgatgcg
     4621 accaaaaaag tcttcaaaca gcgcactgcc accttgcagg ccggcctcaa tcttcagacc
     4681 cgatgccggg ctggcctcaa acccgatgtc cttcaggctg ggtacatctg tctgatacgc
     4741 tggcggtcgc tcggccagtg cgcaggccag tggcttgcag tcatggtcac gcagatgctc
     4801 ggcaccgagc ttcgataacc agttgttttt gtacggggtg aacacgctgt acggcgtgcc
     4861 gccctgggtc agaatgtcgc ggccttcaaa aatcacctgg tctttgcagg tctggaaagc
     4921 aatgccagct tgcgccagcg ctgacttgac ctgcacgtcg cgcgccagcg ccaaaggctc
     4981 atagtcgcgc gccgcaaaaa ccgcttgcgc cttgagctgg gcggccagcc gggcgatctc
     5041 gctgctggcc agcgcgtggc gcacgatcag gccgctgccc gccagaccgc tgagctggcg
     5101 cagtgcctgg tcaagctgca ccagtgattc gcgaatgaac gcaacgcgcc ggtcagcacg
     5161 cggcaaaccg tccagaatgt cgctgtcaaa cacaaaaacg cagtgaagct gacggcagct
     5221 tttgagcgcc agatgcagcg cagcgttgtc gctgacacgc aaatcgcgtc gaaaccacat
     5281 cagtccggta tcaaaaggct tgttcatgtt caccgctaaa attgttccat gactgctgat
     5341 tctgccccca tcaatctgac gaatcatttt ttgatcgcca tgcccgggct gcaggatgac
     5401 ctgtttggcc acagcgtggt gtatttgtgt gaacacagcc agcgtggtgc cctggggctg
     5461 gtcatcaaca agccctgcga tatttccctc aaaggcctgt tcgacaaggt ggagctgcca
     5521 ctaactcgcc aggacctgac cgaggcccag gtgttttacg gtggcccggt tcaaaccgag
     5581 cgtggctttg tgctgcacga ggccactttc gccgatgccg acaagccgtc tgatccggtt
     5641 tactccagca ccatgtcgat tcccggcggg ctggagatga ccacatccaa agacgttctg
     5701 gaggccattt ccaccggcgg tgggccgcgc aagctgctgg tgtcgctggg ctactcggcc
     5761 tggggcgagg ggcaactcga atccgaaatt ggcgaaaaca gttggcttac cgtgttggcc
     5821 gaccgccgcc tgattttcga cacaccggtg gccgagcgtt acgcgcaagc cttgcaactg
     5881 ctggggctgc aagcctggat gctgtcgccc gatgccggac atgcctgacc cggtgactaa
     5941 cgggcggttg ctggctgggg tggccgaccc cgtcgttgca gcgggcctgc aaacctttct
     6001 ggcactg
//
LOCUS       DAOSLL010000253         5973 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_11980_length_5973_cov_4.696519, whole genome shotgun sequence.
ACCESSION   DAOSLL010000253 DAOSLL010000000
VERSION     DAOSLL010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11980_length_5973_cov_4.696519"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(254..1150)
                     /locus_tag="PLB25_11665"
     CDS             complement(254..1150)
                     /locus_tag="PLB25_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOE42268.1"
                     /translation="MIVAYDLLAIGAAACWAVGSVTSVMPARHLGAFAFTRWRMFMVA
                     VMLWAVVAVTGSWRGFGPGYWGVMAISGLIGIFVGDTALFAAVNRLGPRRAGVLFATH
                     AAFSAALGFVLLGERMSLQAFGGAVLTLLGVMSAIVLGRHKDETHAWEADRGHVGMGV
                     ALALLAALCQAVGSLIAKPVMALQVDPVMASAVRVTVATSAHFVLLLAGFKASRAHQA
                     ATLRVLGQTALNGFIAMGVGMTLVLLALEKGDVGMVAVLSSVSPILVLPLLWFQLKRA
                     PAPGAWAGAIMTVVGTALILWR"
     gene            complement(1528..2373)
                     /locus_tag="PLB25_11670"
     CDS             complement(1528..2373)
                     /locus_tag="PLB25_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HOE42269.1"
                     /translation="MPTNSRYWADLSTREFAARQASGELAHTIAVLPVAATEQHGPHL
                     PLSVDTVLVDGVIAAALAHLQADLKVLFLPTQAVGLSPEHARFVGTLTLKNETILRLW
                     TDIGESVAAAGIKKLLLFNAHGGNVAAMDLVARELRARLDLLVYSASWFNLPLPDAQG
                     QDVNALFSPDEHRFGIHAGDIETSMMLALDPAHVDMRQAQNFASASQARASSFELLGN
                     GKSAKLGWQMQDYNPAGAVGNAAAASADKGRALVDAAGRALARLLAEMSRLPADTLRA
                     RPQLP"
     gene            complement(2423..2770)
                     /locus_tag="PLB25_11675"
     CDS             complement(2423..2770)
                     /locus_tag="PLB25_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006161520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HOE42270.1"
                     /translation="MPNLPACPQCTLENTYPDGDHFTCPDCGFEWLQVAAADDGDGAG
                     GPVKDANGKPLANGDTVILIKDLKVRGSSIVLKKGTKIKGIRLPEGAGDHEVDCKTEQ
                     GAFMLKAEFMKKA"
     gene            3718..4548
                     /locus_tag="PLB25_11680"
     CDS             3718..4548
                     /locus_tag="PLB25_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013539213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HOE42271.1"
                     /translation="MTQDLFRQNAYLTECTATVTAITPLGLELDRTVFYPLGGGQAGD
                     TGVLVLADGSQIGIIDTRKGKSEDGSFNGQIAHQPTPDVLEQLLNQDQSGAFKLAVGD
                     TVTARIDWARRLRMMRFHTCSHLLCHIVPQLVNGCSITPDYARIDFNMTEPLDKEALN
                     VAIAQLVAAAHPVTVGAILDAELDANPALVKSMSVQPPRGSGQIRTIQIGGLAPDAAS
                     VGLAGAGQSAGTALIDLQPCGGTHVANTAEIGRIVVTKIEKKSAMTRRVVLGFAPAAP
                     "
     gene            4545..4913
                     /locus_tag="PLB25_11685"
     CDS             4545..4913
                     /locus_tag="PLB25_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HOE42272.1"
                     /translation="MSRGQVHNITGDSFFAWDGDVLVVNILGKPSASKDAIGKPKGTQ
                     LKVSVTAAPQHGKATDHMVRFLAPLFGVNVSGIEVVFGQENVNKQLRIKAPTKLPLVF
                     TPSASNPPTVAATQPRRPRG"
     gene            complement(5134..5451)
                     /locus_tag="PLB25_11690"
     CDS             complement(5134..5451)
                     /locus_tag="PLB25_11690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42273.1"
                     /translation="MRIKLTNMLSIFADRLGQPTSGFEPFVGHGCSPQANLISQLVWE
                     NAWSLTRQAFAYHARNSNIQSHSSRGRESCTFKSVPTVGAGVAPSRRRLFDSGLSGSG
                     HGH"
     gene            complement(5457..>5973)
                     /locus_tag="PLB25_11695"
     CDS             complement(5457..>5973)
                     /locus_tag="PLB25_11695"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOE42274.1"
                     /translation="DQTSAQRREFDLAEFVHEILLTLQPTIKRSPVQIELRVQVPELL
                     IDGYPGPLGQVVSNLIQNSLVHAFGDRSKGLISISAHVETDQVRLSIGDDGCGIPAEL
                     VRRVFDPFFTTRLGKGGSGLGLHIVHNIVTGMLGGEIELQQQEGCGACFVMTFPRCAP
                     AVVTTEPSPAA"
BASE COUNT         1240 a         1884 c         1769 g         1080 t
ORIGIN      
        1 aattcaaaca ttccaagtca ttcgtcgcga gggcgcgact cctacaaatt caaatgggcg
       61 ccgactgttt gtgcaggagt cgcgccctcg cgacgaaggc cgtccaattc cggcttgtcc
      121 ggcttcggtt gatgtactgt cattgtcgcg aaggcggcaa tgacggcgac agcacgagaa
      181 gaaactcgtc gggagcgcta cttgccttgc ggtcaggcgg attgttgtcg gggatcgccg
      241 caaaaggcgg ctatcaacgc caaagaatca gcgccgtgcc caccaccgtc atgatggccc
      301 cggcccaggc gccaggggcc ggggcgcgct tgagctgaaa ccacagcagc ggcagcacca
      361 gaatcggcga caccgatgac aggacagcga ccatgccgac atcgcccttt tcaagcgcca
      421 gcagcaccag cgtcatgccc acacccatgg caataaagcc gttcagcgcg gtttgcccga
      481 gtacccgcag cgtggctgcc tggtgcgccc gagaggcctt gaaacccgcc agcagcaaga
      541 caaaatgggc actggtagcc accgtcaccc gcaccgccga agccatcacc gggtccacct
      601 gcaaggccat caccggcttg gcgatcaggg aacccaccgc ctggcacagc gctgccagca
      661 aagccagcgc gacccccatt ccgacatgac cacggtcagc ttcccaggcg tgggtttcgt
      721 ccttgtggcg gcccagcaca atggccgaca tcaccccaag cagggtcagc acggcgccgc
      781 caaaggcctg caggctcatg cgctcaccaa gcaaaacaaa gcccagtgct gcgctgaacg
      841 ccgcatgggt ggcaaacaag acaccggcgc gacgcggccc aagccggttg acggcggcaa
      901 acagcgcggt gtcgccgaca aaaatgccaa tcaggccgct gatcgccatc accccccaat
      961 agcctggccc gaagccgcgc caggaaccgg tgacggccac cacagcccac agcatgacgg
     1021 ccaccatgaa catgcgccag cgggtaaagg caaaggctcc caggtgccgt gcgggcatca
     1081 ccgaagtgac gctgccgacc gcccagcaag ccgccgcacc gattgccagc aagtcgtaag
     1141 caacgatcat ggcttattca gcaagctgcc agtgcgcccg gcgaaacgct ttggccaggc
     1201 ctgactgcag ccagccccca cacggcccaa atgcctcatc cccgcagcct gtcgattcta
     1261 ggaatcgggc cgcgagaagc gacccgaacg aggtcagctt gcgccagatt tggcggccca
     1321 tagcccggtt gcgagtcaaa tagccggtgc aaaatgggca tctgcagtcg gttcgctgcc
     1381 gctgcgccat cagtgcgaca ggcttcaggc tgccccggtt gagcacttgt tgtcatcggc
     1441 aaactctcat ctgcatttgc tgttttggcc tcattggggc gcacccattg ggtcacggcg
     1501 ctaccggccc aattgaagca tccaggctca tggcagttgc ggccgagctc gcagcgtgtc
     1561 agcaggcagg cgactcattt cagccaacaa gcgcgccagc gcccggccag cagcatccac
     1621 cagcgcccgg cccttgtcag cgctggcagc cgccgcatta ccgactgcgc cagccgggtt
     1681 gtagtcctgc atctgccaac ccaatttggc gcttttgcca ttgcccaaca gttcgaagct
     1741 gctggcgcga gcctgcgatg ccgaagcaaa attttgtgcc tggcgcatgt ccacatgggc
     1801 cgggtccagc gccagcatca tcgaggtctc gatgtcaccg gcgtggatgc caaagcggtg
     1861 ttcgtctggg gaaaacaagg cattcacgtc ctggccttgc gcgtcgggca agggcaaatt
     1921 gaaccagctc gcgctgtaca ccagcaggtc aagccgggcc cgcagctcac gcgcgaccag
     1981 gtccattgcc gccacattgc caccgtgcgc gttgaacaac agcagctttt tgatgccggc
     2041 agccgcgacc gattcgccga tgtcggtcca caggcgcagg atggtttcat tcttgagtgt
     2101 cagggtgccg acaaaacgcg cgtgttccgg gctcagtccg accgcttggg tgggcaagaa
     2161 cagcaccttc aggtcagcct gcagatgcgc cagtgccgct gcaatcacgc catcgaccag
     2221 caccgtgtcc acgctcagcg gcaagtgcgg gccgtgctgc tcggtcgctg ccactggcag
     2281 cacggcaatg gtgtgcgcca gttcgccgct ggcctggcgg gcagcaaatt cgcgggtgct
     2341 caggtcagcc cagtagcgtg aattggtcgg cattggcagg gtggggcaag tgggtaacgg
     2401 gtgaacacca ttgaggccga tttcaggcct ttttcataaa ttcggctttg agcatgaacg
     2461 cgccctgctc ggtcttgcag tccacctcgt ggtcgccagc gccttcgggc aggcggatgc
     2521 ccttgatttt ggtacctttt ttcagcacga ttgacgagcc ccgcaccttc aggtccttga
     2581 tcagaatcac cgtgtcgcca ttggccagcg gcttgccatt ggcatctttg accgggcctc
     2641 ccgcgccatc accatcgtcg gctgcggcta cttggagcca ctcaaagccg caatccgggc
     2701 aagtgaagtg atcaccgtcg ggataggtgt tctccagggt gcattggggg caggcgggca
     2761 aattcggcat gggtttgatg tgctggaaag cggttttttt gtggccccgc aagtgtaaga
     2821 gcatgaccct gacgagggcg gcaatggcag cagggtatct tcaagccatg aacaaagttc
     2881 atcgcaagaa gttcgcgatc aacccctgtc gcgccgtcgg tggcctgcat gtgaccttcg
     2941 gcatgcaatc agcacacgcg ctgcagactc acttgccgcc agcactggca tcgcaaggct
     3001 cgtcaaatac tactgaatta gaagcatgca gcgcttactc aacgggcgct ggaggctgat
     3061 ttggcatatc gatctgccac ccctggcccg tgcttgatca atgggttgcc gcatgcagcg
     3121 caccgcctgc gacacgtcaa cgcgcaagcg acgcagtgat tttggggtcg gatcccaatg
     3181 gctgacagca ggcatctgcg agtgagcacg gcctatccag ccattgggtc agaccccaac
     3241 atcgcgggtt ggggctgctg atgctctcaa cacaatagct gcccacgcat actcagtaag
     3301 gtctggcggc cgatttggca tataagccgt taccccctgg cccctgaaat tggtgaattg
     3361 gggtcagagc ccaatttcct ttgcatccag gttccccgca tcggctgttg gccggaaact
     3421 gggatccgac ccaaactcgc tgcacctgcg ctgtgccctt ggtttgataa cccaatatcg
     3481 ccgtctgaag ctgcgcagga tatcaaaaac aatagctgct cacgcatgcc cggtgcgggc
     3541 tggcggccga tttgacatgc aagccgtgac tttgatcggt caccccaggc gttgacaatg
     3601 gcgtgacgca attgcattca atggcgacca cgacgaccgg gcactttttc aagccaaatg
     3661 tgccaacctg gctcatctgg tgctcgaccc tgttgcgcag cggcgaaaat gggcggcatg
     3721 acacaagacc tttttcgcca aaacgcttat ctgaccgaat gcaccgccac ggtgaccgcc
     3781 atcacgccct tggggctgga acttgaccgc accgtgtttt acccgctggg cggcgggcag
     3841 gcgggcgaca ctggcgtgct ggtgctggca gacggcagcc agatcggcat catcgacacc
     3901 cgcaagggca aatcggagga cggcagcttc aatggccaaa tcgcccacca gcccacacca
     3961 gacgtgcttg agcagctatt gaatcaggat caatctgggg ccttcaagct tgcggttggc
     4021 gacaccgtga cggcccgcat cgactgggca cgccgcctgc ggatgatgcg ttttcacacc
     4081 tgcagccacc tgctgtgcca catcgtgccg cagttggtca atggctgctc gattacgcca
     4141 gactacgccc gcattgactt caacatgacc gagccgctcg acaaggaggc gctgaatgtt
     4201 gccattgcgc aactggtggc cgccgcgcac ccggtcacgg tgggtgccat ccttgatgcc
     4261 gagcttgatg ccaatccggc gctggtcaaa agcatgtcgg tgcagccgcc gcgtggcagc
     4321 gggcagattc gcacgattca gattggtggc ctggcaccgg atgcagcttc ggtgggtttg
     4381 gccggcgcag gccagtcagc gggcaccgca ctgattgacc tgcaaccctg cggcggcacg
     4441 catgtggcca acaccgccga gattggccgg atcgtcgtga ccaagatcga aaagaagtcg
     4501 gccatgacgc gccgggtggt gctgggcttt gccccggcag cgccatgagc cgaggtcagg
     4561 tccacaacat caccggcgac agtttttttg cctgggacgg tgatgtgctg gtggtcaaca
     4621 tcctgggcaa acccagcgcc agcaaggacg cgattggcaa acccaagggc acccagctca
     4681 aggtcagcgt caccgcagcc ccccagcacg gcaaggccac cgaccacatg gtgcggtttc
     4741 tggcgcccct gtttggtgtg aatgtgtccg gcatcgaggt ggtatttggg caggaaaacg
     4801 tcaacaaaca gttgcgcatc aaggcaccaa ccaagctgcc cctggtgttc acgccaagcg
     4861 catcaaaccc gccgacggtg gccgcgacgc agccccggcg gccacgcggc taggcgcttg
     4921 tcagactgct gaatcgagcc gcgaccccgg caaggccagt ttgcaccgga attgccggca
     4981 catagcccga tggtgagtca aacggccggt tcaaaatggg ccgcttcggt cagttcgcag
     5041 ccgatccgcc ataagtccgg caggcgccgc gccgcgccag cgatttgcat tgacgcagtg
     5101 cgcccaaacc ggacgagccg aatgggaaag ctatcaatgg ccatgtcctg agccggacaa
     5161 gccggaatcg aacagccttc gtcgagaggg tgcgactcct gcaccaacag tcggcaccga
     5221 tttgaatgtg caggactcgc gccctcgcga cgaatgactt tgaatgtttg aattcctcgc
     5281 atggtatgca aaggcttgtc ttgtcaggga ccaggcattt tcccagacaa gctgtgaaat
     5341 gaggttcgcc tgcggcgaac agccatggcc tacaaaaggt tcaaaaccgg atgtcggctg
     5401 cccgagacgg tcggcaaaga tcgacaacat gttggtaagt ttgatgcgca tggtgcctaa
     5461 gcagcaggcg acggctccgt tgtgacaacg gcgggggcac aacgcggaaa agtcatcaca
     5521 aagcaagcgc cgcaaccctc ttgctgctgc aactcgattt ctccaccgag catgccagtg
     5581 acaatgttgt ggacaatgtg aagccccagg ccgctgccac ccttgcccag acgggtggtg
     5641 aaaaacgggt caaacacgcg gcgcaccaac tcggcaggaa tgccgcaacc gtcatcacca
     5701 atgctcagcc tgacctggtc tgtctcaacg tgcgcgctga tgctgatcag gcctttgctt
     5761 cggtcaccaa aggcatgaac caggctgttc tggatcaggt tggaaaccac ttgtccgagc
     5821 ggccccggat agccgtcgat gagcaactca ggcacctgta cccgcagttc aatctgcacc
     5881 gggctgcgct tgatcgttgg ctgcagcgtc agcagtatct catgaacaaa ttcggccaga
     5941 tcgaattcgc gcctttgcgc gctggtctga tca
//
LOCUS       DAOSLL010000254         5964 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12003_length_5964_cov_4.117786, whole genome shotgun sequence.
ACCESSION   DAOSLL010000254 DAOSLL010000000
VERSION     DAOSLL010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5964)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12003_length_5964_cov_4.117786"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..163)
                     /locus_tag="PLB25_11700"
     CDS             complement(<1..163)
                     /locus_tag="PLB25_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018006411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HOE42275.1"
                     /translation="MKIETLAVHAGYTPDPTTKAVAVPIYQTVAFAFDDTQHGADLFD
                     LKVAGNIYSR"
     gene            321..1760
                     /locus_tag="PLB25_11705"
     CDS             321..1760
                     /locus_tag="PLB25_11705"
                     /inference="COORDINATES: protein motif:HMM:NF024985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HOE42276.1"
                     /translation="MNTGIQFTPLLLALSISGTLAQGTEQRSTLEDQQDVAITIYNSD
                     LALVKDHRKIQLTPGASSLAFRDVSARMRPETALMRSLNAPGSLSVLEQNFDFDLLTP
                     QKMLEKYVGQVVSVVRTNPATGQETSEIATVLSANNGVVLKIGQRIETGMPGRLVFGN
                     VPANLRDRPTLVMSLSNTGAAQQDVELSYLTGGLSWKADYVAELNATDDKMDVSGWVT
                     LTNTSGATYSRAKLQLVAGDVNRVAPQFQRSAQVMMAPAPAPAAKADMAQESLFEYHL
                     YTLGRPTTLAENQTKQVLLLTGSGVPVRKELLFKGSDYYYQSSVGSIGQKMKVGVFVE
                     FDNKESGQLGIPLPKGIVRVYKKDTAGNAQFVGEDSVDHTPKNETVRLKLGEAFDVTA
                     DKKQTDFKKLAGSGRFNYVFESAYEIVLKNAKKEAVMVTLQEPMPGDWQILSQSLAHT
                     KGASNTAVWKVSVPAEGSSTLTYRALVRF"
     gene            complement(1900..3096)
                     /locus_tag="PLB25_11710"
     CDS             complement(1900..3096)
                     /locus_tag="PLB25_11710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfB/YjiJ family MFS transporter"
                     /protein_id="HOE42277.1"
                     /translation="MRDQIARLKVLGAGIFSLLLVLGVARFSYTPLLPLMQQQAGLGV
                     AEAGWLAAINYGGYLSGAVIASLISDLVLKDRLYRIGMVLAIVSTVVMGLTTNVVVWA
                     LSRYVAGLSSAAGMLLGTGLILNWLIRHNHRSELGIHFAGIGLGIAGCAAAVTLWSPW
                     LNWREQWFAFTALGCLLLLPALLWLPRPDQTGLTMSGQKMHDQPPSTLYLRIFMAAYF
                     CAGFGYVVSATFIVAIVNKLPGLAGQGNLAFLAIGIGAAPACINWDLIARRTGDLNAL
                     ILAALLQIVGILLPVLVGGLPATLFGALLFGGTFIGMVSLVLSMAGRYYPTRPAKMMG
                     KMTLSYGAAQIVGPAVTGSLAERLGSYNAGLYFAAAVMVLGTLLLLVLKLVERRDALH
                     QGHGQQ"
     gene            complement(3128..3520)
                     /locus_tag="PLB25_11715"
     CDS             complement(3128..3520)
                     /locus_tag="PLB25_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HOE42278.1"
                     /translation="MKTVIRLFFKTLRLALGPFMLLWERLSRPTGLMRSPSLQAQVDQ
                     QCRDLVLYQFTTCPFCIKVRQEMRRLSLNIERRDAQHDAQNRAALVLGLGQPKVPCLK
                     ITDAAGQVQWLAESGAIMAYLRGRFAAA"
     gene            complement(3535..3963)
                     /locus_tag="PLB25_11720"
     CDS             complement(3535..3963)
                     /locus_tag="PLB25_11720"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOE42279.1"
                     /translation="MSNACLIAIDGSEPSLRAVNYIIGDAINRRTPPMVHLVNVQPAL
                     STDVSRFIDAKTIEDFHREAGDTSLASAKEKLTQAGLAYSAHILIGEVAPTLVEFAST
                     HGCGLIVMGAHGFGSLVGLFMGSVTVKVVQLSKVPVVLIK"
     gene            complement(3982..4695)
                     /locus_tag="PLB25_11725"
     CDS             complement(3982..4695)
                     /locus_tag="PLB25_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003799530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOE42280.1"
                     /translation="MPDFSSSLFWVAVLQIIAIDIMLGGDNAVVIALACRKLPENQRN
                     KGIFWGVIGAIGLRVVMIFFALQLLAVPYLKIVGGLLLFWIGIKLLQPEHGDEHNHID
                     GGTTLMAAIKTIVVADAVMSLDNVIAVAGASHGSMVLVTFGILVSIPIVVWGSKLVLS
                     MMDRFPVVITLGAGLLGWIGGGMLLTDLATPASIAKGIPYAHYVFAGTGALLVVIAGK
                     WLGRRKGRRASVELPASNH"
     gene            4953..>5964
                     /locus_tag="PLB25_11730"
     CDS             4953..>5964
                     /locus_tag="PLB25_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HOE42281.1"
                     /translation="MQLPFELSLGWRYTRAGRATRRNGFISFISGVSMLGIALGVAAL
                     IIVLSVMNGFQKEVRDRMLGVVSHIEVFAPNAAALPDVARTLTEIQANTAVIGAAPFI
                     ATQALLARGEDMKGTIVRGIDPVREPDVTDLAGDLKDSAMSRLVAGEFGIVLGGELAR
                     SMGVRSGDKITLIAPSGQVTPAGVVPRLKQMTVVGTFDSGHYEYDSGLVLMHLDDAAR
                     IFRLEGPTGVRVRLKDLHQARAVAAQLTTTLTGNLLIRDWTRQNRTWFAAVQLEKRMM
                     FIILTLIVAVAAFNLVSTLVMTVTDKRADIAILRTLGASPASIMGIFVVQGAMVGVIG
                     TLA"
BASE COUNT         1396 a         1765 c         1682 g         1121 t
ORIGIN      
        1 tgcggctgta aatattgcca gctaccttca ggtcaaacag gtcagcgcca tgctgggtgt
       61 cgtcaaacgc aaaggcaacc gtctgataaa tcggcaccgc aaccgctttt gttgttgggt
      121 ctggtgtgta gccagcgtga actgccaaag tttcaatttt catgagatgc ccatccaatg
      181 caaaagtcgg gaatcttaga tcaagcccgg agattgcgcc tgaccaccgt agcgcgttgc
      241 cagcaaaaag gtgaaatcag acaggtacaa cagcaaaaag ggcaaaattg cagcttccaa
      301 aaatcaccca gggtcacacc atgaacaccg gaattcaatt taccccactc ttgctggcct
      361 tgtcgatatc gggcaccctg gcccagggca cggagcagcg cagtactctg gaagatcagc
      421 aagatgttgc catcacgatc tacaacagcg atctggcctt ggtcaaggac catcgcaaga
      481 ttcaattgac accaggcgca tcgtcgttgg cattcaggga tgtgagtgcc agaatgcgtc
      541 ccgagactgc cttgatgcgt agcctgaacg cacctggaag cctcagcgtg ctggagcaga
      601 attttgattt tgacctgctg acaccgcaaa aaatgctgga gaaatatgtc ggacaagtcg
      661 tcagtgtcgt ccgtaccaat ccggcgaccg gacaagagac cagcgaaatt gccacagttt
      721 tgtctgccaa caacggtgtc gtcctgaaga tcgggcagcg catcgagacc ggcatgccgg
      781 ggcggctggt ttttggcaac gtgcccgcca atctgcgtga tcggccgacg ttggtgatga
      841 gcctgagcaa cacgggcgcc gcacaacagg atgtggagtt gagctacctc accggtgggc
      901 tttcctggaa agctgactat gttgctgaac tcaatgccac cgacgacaag atggatgttt
      961 ctggctgggt caccctgacc aacaccagcg gtgccaccta cagcagggcc aaactgcaac
     1021 tggtagccgg tgatgtcaac cgtgtcgcac ctcagtttca gcgcagcgcc caagtcatga
     1081 tggcccccgc acctgcgccc gccgcaaagg ctgatatggc ccaggaaagc ttgtttgaat
     1141 accacctcta cactctgggc cgccccacca ctctggcaga aaaccagacc aagcaggtat
     1201 tgctgctgac cggctcaggt gtgccggtgc gcaaggaact cctctttaag ggcagtgact
     1261 actattacca aagcagcgtg ggcagcattg gtcaaaagat gaaggtcggt gtttttgtcg
     1321 agtttgacaa caaggaaagt ggccaactgg gcatcccctt gcccaaaggg attgtgcggg
     1381 tttacaagaa agatactgct ggcaacgccc agtttgttgg cgaagacagc gttgatcaca
     1441 ccccgaaaaa cgagacagtt cggttgaagc tcggggaggc atttgacgtg accgccgaca
     1501 agaagcaaac cgacttcaaa aagctggcag gttctggcag attcaactat gtttttgaaa
     1561 gcgcctacga aattgtgctc aaaaatgcca aaaaagaagc cgtgatggtt accctgcagg
     1621 agccaatgcc tggcgactgg cagattttgt cgcaaagcct ggcacacacc aaaggtgcca
     1681 gcaacacggc ggtctggaaa gtcagcgtgc cagccgaagg cagcagcacc ctgacttacc
     1741 gcgcattggt acggttttag cctttggtcg aaaacaagtt acgcatttgg ccgttggatt
     1801 tgggatgcaa tgccaggcgc aaagcgcgac atgtggcgag ccacattggc agtttgcaac
     1861 aacgcagggc gcccaaaccc gacgagccaa atgggaaagt tattgttgac catgtccttg
     1921 gtgcaaggcg tcgcgtcgtt caaccaattt caagaccagc agcagcagtg ttcccagcac
     1981 catgaccgct gcggcgaaat acagtccggc gttgtagctg ccaaggcgct ctgccaatga
     2041 tcctgtgacg gccggcccaa cgatctgcgc cgcaccgtaa gacagcgtca ttttgcccat
     2101 catcttggcc gggcgtgtcg ggtaatagcg cccggccatg cttagaacca gactcaccat
     2161 gccgataaaa gtgccgccaa acagcagcgc accaaacagg gttgccggca agccgcccac
     2221 cagaaccggc agcaaaatgc cgacaatctg cagcaatgca gccaggataa gggcgttgag
     2281 gtcaccggtg cgccgggcaa tcaagtccca gttgatgcag gctggcgcag caccgatacc
     2341 aatcgccaga aaagccaggt tgccctgccc tgccagtcca ggcaacttgt tgacaattgc
     2401 cacaatgaat gtggcactca ccacgtagcc gaacccggca caaaaatatg cggccataaa
     2461 aatccgcaga tacagtgtgc tcggcggttg gtcgtgcatt ttttgcccgc tcatggtcag
     2521 gccggtttga tccggcctgg gcagccaaag cagggctggc aacagcagca gacaacccaa
     2581 ggcggtaaag gcaaaccatt gctcgcgcca gtttagccag ggactccaca gcgtcacggc
     2641 agccgcgcag cctgcaatgc ccaagccaat gccagcaaaa tggatgccca gctcgctgcg
     2701 gtggttgtgg cgtatcagcc aattcagaat caaaccggtg cccagaagca tgccggcggc
     2761 actgctcaaa cccgccacat agcgcgacag cgcccagacc acaacattgg tagtcagccc
     2821 catgacaacg gtactgacga ttgccagcac catgccgatg cggtagagcc gatctttcaa
     2881 caccaaatcg ctgatcagcg aggcaatcac agcaccactg agataaccgc cgtagttgat
     2941 ggctgccagc cagccagcct cggccacacc caggcccgct tgctgctgca tcagtggcaa
     3001 caaaggcgtg taagaaaagc gcgccacgcc cagcaccagc agcagactga aaatacccgc
     3061 tccaagcacc ttcaggcggg caatttgatc cctcatgcca ctgatcggta gcagattgtg
     3121 atggccatca tgcggcggca aagcgtccgc gcaaataggc catgatggcc ccggactcag
     3181 ccagccactg cacctggcca gcggcgtcgg tgattttcag gcacggcact ttgggctggc
     3241 ccaggccgag caccagcgct gcacggtttt gcgcgtcatg ctgcgcgtca cgccgctcaa
     3301 tgttgagcga caggcggcgc atctcctggc gtaccttgat gcaaaagggg caggtagtga
     3361 actggtaaag caccaggtcg cgacattgct ggtccacttg cgcctgcagg cttggtgaac
     3421 gcatcagacc agtcggccga ctcagccgtt cccacagcag catgaaaggc ccgagcgcca
     3481 gccgaagcgt tttgaaaaaa agccggatca cagttttcat gtcgggtaag ctggttactt
     3541 gatcaacaca acaggcacct ttgagagctg gaccaccttg acggtgaccg atcccatgaa
     3601 caggcccacc aaggaaccaa agccatgggc acccatgaca atcagtccgc agccgtgcgt
     3661 gctggcaaac tcgaccaggg tgggggccac ctcgccaatc aagatatgtg ccgaatacgc
     3721 cagtcctgcc tgggtgagtt tttctttggc agaggcgagc gaagtgtcac ccgcctcccg
     3781 atgaaaatcc tcgatcgttt tggcatcaat gaagcgagac acgtcggtag acagggcggg
     3841 ctgcacattc accaggtgaa ccatcggcgg ggtgcggcgg ttgattgcat cgccgatgat
     3901 gtaattcacc gccctgagcg agggctcaga accatcgatg gcaatcaggc atgcgttgct
     3961 catggtcaat ctccttgctt gtcaatggtt tgatgctggc agttcgacac tggcgcgccg
     4021 acctttgcga cgcccaagcc acttgcccgc gataacgacc agcagcgccc cggtgccggc
     4081 aaaaacatag tgagcgtaag ggatgccctt tgcaattgac gcgggggtgg cgaggtcggt
     4141 cagcagcatg ccaccaccaa tccagccgag caagccggcc cccagcgtga tgaccaccgg
     4201 aaaacggtcc atcatcgaca gcaccagctt gctgccccag accacaatcg gtatcgacac
     4261 cagaatgccg aaagtcacca gcaccatcga gccgtgcgag gcacctgcca cggcgatcac
     4321 gttgtccaga ctcatcaccg catcggcaac cacaatcgtc ttgatggcag ccatcagggt
     4381 ggtgccaccg tcaatgtggt tgtgttcgtc gccgtgttcg ggctgcagca gcttgatgcc
     4441 aatccagaac agcagcagcc cgccgacgat cttcagatag ggcacggcca gcagttgcag
     4501 ggcaaagaaa atcatcacca cgcgcagccc gatagccccg atcacgcccc aaaaaatgcc
     4561 cttgttgcgc tggttttcgg gcagcttgcg acaagccagg gcgatcacca ccgcattgtc
     4621 gccaccgagc atgatgtcga tggcaatgat ctgcagcacg gcgacccaga aaagcgaaga
     4681 agaaaaatca ggcatgtcga tgaaatctga agataaaagc gctgagcgtg ccccggcgca
     4741 tggtctgcaa gtcaaaaaag tgctactgta tccgagaacg tccgacagac gcttttgcgc
     4801 cgggatatgc cctgatgagc gcgcccggac agggaaaaat atcggcgcat aatcaacctt
     4861 cgttcatcca cctgttgaac caactgttgc atgcacccac ttaccgctac cgtgtttttc
     4921 atcgccaggc gatgcccttg ccccggtctc tgatgcaact cccctttgaa cttagtcttg
     4981 gctggcgcta cacgcgcgct ggccgcgcca cccggcgcaa tggttttatt tcgtttatct
     5041 ccggtgtttc catgctgggc atcgccttgg gcgtggcggc gctgatcatt gtgctcagtg
     5101 tcatgaacgg tttccagaag gaagtgcgtg accgcatgct cggggtggtc tcgcatatcg
     5161 aggtatttgc ccccaacgct gcggcactgc ccgatgtggc ccgcacgctg accgaaatcc
     5221 aggccaacac cgccgtcatt ggtgctgcac ctttcatagc tacccaggca ctgctggcaa
     5281 ggggtgagga catgaaaggc accatagttc ggggtattga cccggtccgc gaacccgatg
     5341 tgaccgacct ggcgggcgac ctcaaagact cggccatgag ccgcttggtt gctggcgagt
     5401 ttggcattgt gctgggcggt gaactggcgc gcagcatggg ggtgcgcagc ggcgacaaga
     5461 tcaccttgat cgccccgagc ggtcaggtta ccccggctgg cgtggtgccg cgcctgaaac
     5521 aaatgacggt ggtcggcact tttgactcgg gccactatga atacgactcg gggctggtgc
     5581 tgatgcacct cgatgacgcg gcgcgcattt tccgcctgga aggcccaacc ggtgtccggg
     5641 tgcgactcaa ggacctgcac caggcgcgcg cagtggccgc gcaactcacc accacactca
     5701 ccggcaacct gttgattcgc gactggacgc gccagaaccg cacctggttt gccgccgtgc
     5761 agcttgaaaa gcgcatgatg ttcatcatcc tcacgctgat cgtggcggtg gccgccttca
     5821 atctggtcag caccctggtg atgacagtca ccgacaagcg ggctgacatc gccattttgc
     5881 gcaccctggg tgccagcccg gccagcatca tgggcatatt tgtggtgcaa ggtgccatgg
     5941 tgggcgtcat cggcacgctg gccg
//
LOCUS       DAOSLL010000255         5950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12046_length_5950_cov_4.493130, whole genome shotgun sequence.
ACCESSION   DAOSLL010000255 DAOSLL010000000
VERSION     DAOSLL010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12046_length_5950_cov_4.493130"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="PLB25_11735"
     CDS             <1..534
                     /locus_tag="PLB25_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE42282.1"
                     /translation="AVQLAMWVAIPALLVQAGLDKAQHWWVYLPAVLASFVVMGGTLF
                     PLEKRGYLRAVFLGAIGLIGLVQLGLLWVASAGPSIAMLAFLLFVFFCGFNVLEASQP
                     SLASRVAPAHARGAALGVYNTLQSLGFFVGGAVGGWLIKNTGLHGLFVACSVGMVLWL
                     AVAWPMQAPSRASGNTP"
     gene            571..1071
                     /gene="ssb"
                     /locus_tag="PLB25_11740"
     CDS             571..1071
                     /gene="ssb"
                     /locus_tag="PLB25_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HOE42283.1"
                     /translation="MASVNKVILVGNCGRDPEIRYLPSGQAVANVSVATSSRRKDRNT
                     GETVEDTQWHRVTFYDRLAEIAGEYVKKGRPIYVEGRLKYGKYTDQSGVEKNTVDIVA
                     TELQLLGGREGMGGPSEGDEGGGAPAPRRMAPSAPPRAPAPAPRQAPAPRPASGFDDM
                     DDDIPF"
     gene            complement(1149..2996)
                     /locus_tag="PLB25_11745"
     CDS             complement(1149..2996)
                     /locus_tag="PLB25_11745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HOE42284.1"
                     /translation="MTRLKTAGKFLALTATALGCALACAQTPTTLKGAVERAILQNPE
                     VKYRFHNLEAAQEERKSAEGAWFPRVDLEAATGTYNTLRPSLASTLGYSGNRASIQLR
                     QVLFDGFATRNEVRRLSYSQQTAYYELLSASNQTGLETARAYLDVLRYRDLLTLAASN
                     YSTHLEVHNRLEQKTKAGVGRRVDLEQASGRLALAESNWLTEASNLHDVSARYQRLVG
                     DLPATSLAPADPLKAGVTSGATFLADAVKKNPDFLGAVSTIRAYRADAAFRRSAYAPT
                     VELRARQSYETNQSGVTGDYRDSALELVLNYNLYRGGADKAKIKQYLHKLDSAFDLRD
                     KTCRDLWQTGQIAYNDSTRLANQIQLLAQHELSTSKARQAYQQQFDIGQRSLLDLLDT
                     ENELYQARRALANAEYDQKLAEVRLLATNGSLLSALKLQAMPVSEPDPSGGAEEEDGL
                     MQCSNFMPVVPVLDRTVEARPVVEPDPIAPTAPVPLPSPVVVTPPPPAPCTQLPQWVD
                     GWISAWNGKQLTSYLGYYADKFTPALGMSRSAWESLRKKRINKQGDIKAVISDIKPLS
                     CDGKTAEVAFKQEYGSVDYRDTVEKTLSLEQIDNNWKILRETVTKGRTF"
     gene            3190..3918
                     /locus_tag="PLB25_11750"
     CDS             3190..3918
                     /locus_tag="PLB25_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008164886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HOE42285.1"
                     /translation="MLFNTFHGAVNLPSLRISPIRRPVFGLLVCAFAFWMIAAAYGGA
                     DFDRLLVSLTQRWGGGAATKFTAWRAMVVGAATATDADRIKRANDFFNRQMQFGDDSA
                     IWGQVDFWATPMESLGKGAGDCEDFAIAKYFTLKEMGVASEKLRLIYVRAKTGTSDVA
                     QSQAHMVLAYYAQPDAEPLVLDNLIGEIRPASRRLDLVPVFSFNSEGVYTGTPGKEGA
                     VSVGVGRLSRWEDLLKRARLEGFE"
     gene            3945..5861
                     /locus_tag="PLB25_11755"
     CDS             3945..5861
                     /locus_tag="PLB25_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE42286.1"
                     /translation="MSMYRQLWLAIMVSTLLALAGSMLASMFSARSYLESQLAIKNTD
                     NATALALSLSQSNPDPVMVELVVTSLFDRGHYEMIRVTDPSGATLVERIASDNEWDAP
                     HWFVRLLPIKAEAGRAQISNGWKQFGTVTLVSHSRFAYGALWKSAYEMALALAIAGLV
                     GGYLGSLVLRRLKGPLDAVIEQAKAITLRRFVTIDEPKVPELRQLAVAMNATVGRLKS
                     MFDEEADRLDAVRREANCDALTGLANRSYFMARLRETVAADDSSGGAVFIARLANLAT
                     VNQTLGRDATDELLRAYGEALAEVARKYPEGLAARLNGADFALLVPALASPQETAHQL
                     LQSLVQVASAYLPEYPSTWLGCGHFPRGVETAAILAQVDAALAAVEAEGRNGMRLVDM
                     RQGDEAPRSSEEWSNIIRRALEQRWVRLVSFPVASLDGGLIHRECPLRLMFDPSGEWQ
                     PAGRFLPVAERLRLTPQLDLAAVALGLDELEAKPALPGLAINLSASSLELTEFRTELR
                     QLLKRRSGTSRLWLEVAESGVLAHFAAFRELCSELRDVGCKLGVEHFGRQFSEIGRLH
                     DLGLDYLKVDGSFIRGLDQNIGNQTFLKGLSTIAHGIGLQVIAEGVTSAAELQALGLL
                     GFDGSTGPAVQDPS"
BASE COUNT         1078 a         1603 c         1941 g         1328 t
ORIGIN      
        1 gccgtgcagc tggcgatgtg ggtggcgatt cctgccctgc tggtgcaggc cggactggac
       61 aaggcgcaac actggtgggt gtatctgccg gcggtgctgg cttcctttgt ggtgatgggc
      121 gggaccctgt ttccgctgga aaagcggggc tatctgcgcg cggtgtttct gggagcgatt
      181 ggcctgatcg gtctggtgca actgggcttg ctgtgggtgg cgtccgccgg tcccagcatt
      241 gccatgctgg cgttcttgct gttcgtattt ttctgcgggt tcaatgtgct ggaggccagc
      301 cagcccagcc ttgcctctcg cgtggcaccg gcccatgccc ggggtgcggc gctgggggtg
      361 tacaacacgc tgcagtcgct gggtttcttt gtcggcggcg ccgtgggcgg ctggctgatc
      421 aagaacacgg gtctgcacgg attgttcgtg gcctgcagtg tgggcatggt gctgtggctg
      481 gcggtggcct ggcccatgca ggcgccgtcc agggcgtctg gcaacacgcc ataatcaccc
      541 taaattattg tgaatctgga gtaattgctt atggcatccg tcaacaaagt catcctggtc
      601 ggcaactgcg gccgtgaccc cgaaatccgc tacctgccct cgggtcaggc cgttgccaac
      661 gtgagtgtgg ccaccagcag ccgccgcaag gaccgcaata ctggcgagac cgtggaagac
      721 acccaatggc accgcgtcac gttttacgat cgtttggccg aaatcgccgg tgaatatgtc
      781 aagaaaggcc gtcccattta cgtggaaggt cgactgaagt acggcaagta caccgaccag
      841 tctggcgtgg aaaagaacac cgtggacatc gtcgctacgg aattgcagct cctgggtggt
      901 cgtgaaggca tgggcggccc gagtgagggc gatgaggggg gtggtgctcc cgcaccccgc
      961 cgcatggcgc cttctgctcc cccgcgtgca cccgcaccgg caccccgaca ggcccccgca
     1021 ccacggccgg ccagtggctt tgatgacatg gatgacgata ttccgtttta gacccgagtc
     1081 agcttcggct tggacagaaa aaaagcgacc gcatgcggtc gctttttcat tggagcccag
     1141 gcccagggtt agaacgtgcg ccccttggtg acggtctcac gcaggatctt ccagttgttg
     1201 tcgatttgct ccagggaaag ggtcttttcc acagtgtccc ggtagtccac cgaaccgtac
     1261 tcctgcttga aggccacttc tgcggtcttt ccgtcgcagg acagcggctt gatgtcgctg
     1321 atgactgcct tgatatcgcc ctgtttgtta atgcgcttct tgcgcagcga ctcccatgca
     1381 ctgcggctca tacccagcgc aggagtgaat ttgtcggcgt aatagcccag gtaactggtg
     1441 agctgtttgc cattccaggc agaaatccag ccatctaccc attggggcag ttgggtgcaa
     1501 ggggctgggg gcggtggcgt tacgaccacg ggggatggca agggcacagg cgcagtcggc
     1561 gcgatcgggt ccggctccac gacaggtctg gcttccacgg tgcgatccag caccggaaca
     1621 acaggcatga agttgctgca ctgcatcagc ccgtcttcct cttcggcgcc gccggacgga
     1681 tcaggttcag acacaggcat ggcctgcaat ttgagcgcac tcagcagcga gccattggtc
     1741 gccagcaggc gaacttcggc gagcttctgg tcgtattcgg cgttggccag cgccctgcgc
     1801 gcctggtaca actcgttctc ggtgtccagc agatccagga gagagcgctg cccaatgtca
     1861 aattgctgct ggtaggcttg gcgggctttg gaggtggaca gctcatgctg ggccaacagc
     1921 tggatttgat tcgccaggcg cgtggagtcg ttgtaggcga tctgtccggt ctgccagagg
     1981 tcccggcatg ttttgtcgcg gagatcgaag gcactgtcga gcttgtgcag gtactgtttg
     2041 atcttcgcct tgtcggctcc gccacggtac aggttgtagt tcagcaccag ttccagcgcc
     2101 gagtcacggt agtcgccggt cacaccactc tggttggttt cataactctg gcgcgcacgc
     2161 agttcgacgg tgggggcata agccgaacgc ctgaaagcgg catccgcccg gtaggcgcgt
     2221 atggtggaca ccgcacccaa aaagtccggg ttctttttga ccgcatctgc gagaaacgtg
     2281 gcgccagagg tgactcccgc cttgagcgga tccgccggag ccagtgaggt tgcaggcagg
     2341 tcacccacga gccgttggta acgcgcagag acgtcatgca gattgcttgc ttcggtcagc
     2401 cagttggatt cagccagcgc cagtcggcca gacgcctgct ccaggtcgac cctgcgccct
     2461 acaccggctt tggttttctg ctcaaggcgg ttgtggactt ccaggtgggt gctgtagttg
     2521 ctggctgcca gggtcagcag gtcgcggtag cgcagaacgt ccaggtacgc gcgggcagtc
     2581 tccagtccgg tctggttgct ggcagacagc agctcatagt aggcagtttg ctggctgtag
     2641 ctcagtcggc gtacctcgtt gcgcgttgcg aaaccgtcaa acagaacctg ccgtagctgg
     2701 atggaggccc ggtttccaga gtagcccagt gtggacgcca gcgaagggcg cagtgtgttg
     2761 taggtgccag tggcggcttc caggtcaacc cgagggaacc acgcaccttc ggctgatttg
     2821 cgttcttcct gggctgcttc gagattgtgg aagcggtatt tgacttccgg attttgcaaa
     2881 atggcccgtt cgacggcgcc tttgagcgtg gttggggttt gggcgcatgc cagggcacat
     2941 cccaaagcag ttgccgtgag cgcaagaaat ttgcctgcag tcttcaatcg agtcatgtct
     3001 tgtccctgta agcgtttttt tgcagtgtgc caagtgcgcc tgcatgcgcc aaaggctgtg
     3061 ctaacctcta ccgaaaggta ataacctggc attgtcgctt gatttggcgc gggacaggct
     3121 tacacaacgg tacaaaaccc tcaggcgcag tgcgacccat ttcacgcaaa tggtgaaagc
     3181 ttcgccgggt tgcttttcaa cacctttcac ggcgcagtca atttgcccag tttaaggatc
     3241 tccccaattc gtcgcccggt cttcgggctg ctggtgtgtg cgttcgcgtt ctggatgatt
     3301 gcggctgcct acggcggcgc cgattttgac cggttgctgg tttcactcac gcagcgttgg
     3361 gggggcgggg cggcaaccaa gttcactgca tggcgggcca tggtggttgg cgcggctacg
     3421 gcgaccgatg ccgacagaat caagcgcgcc aatgattttt tcaatcgtca aatgcagttt
     3481 ggtgacgaca gtgcaatttg gggccaggtg gatttttggg ccactcccat ggaaagtttg
     3541 ggcaagggtg ctggtgactg tgaagacttt gccattgcca aatatttcac attgaaggaa
     3601 atgggtgttg cttcagaaaa gctgcgcctg atttatgtac gggccaagac cggcacttcg
     3661 gatgttgcac agagtcaggc acatatggtg ctggcgtatt acgcgcaacc cgacgccgag
     3721 cctctggtgc tggataacct gataggggaa atccgccctg cctcacggag gctggacctg
     3781 gtgcctgtct tcagctttaa cagcgaaggg gtgtacaccg gaacgccggg caaagagggc
     3841 gccgtctcgg tgggcgtggg gcgtctgtcg cgttgggaag atttgctcaa gcgcgcacgc
     3901 ctggaaggat ttgaatgaac cgcagttatg tgaggaattg acatatgtcc atgtaccgtc
     3961 aactatggtt agccatcatg gtcagcacgc tgctggcgct ggccggaagc atgctggcgt
     4021 cgatgttcag tgcgcgcagt tatctcgagt ctcagctggc catcaagaac acggataacg
     4081 caaccgcatt ggcactctcc ctaagccaga gcaatcccga tccggtcatg gtggagttgg
     4141 tcgttacctc gttgtttgat cgggggcact atgaaatgat tcgtgtcacg gaccccagcg
     4201 gcgcgacctt ggtggagcgg atcgcttccg ataacgaatg ggatgcgcca cactggtttg
     4261 ttcgactgct tcccatcaaa gccgaagctg ggcgggccca gatcagcaat ggctggaaac
     4321 agtttggtac ggtgacactg gtgagccaca gccgttttgc ctatggcgcg ctgtggaaga
     4381 gtgcttatga aatggcattg gcattggcta ttgctgggtt ggtcggtggt tatctcgggt
     4441 cgctggtgtt gcgccggctc aaagggccac tggatgccgt gattgaacag gcaaaggcca
     4501 ttacgctgcg ccggtttgtg acaattgacg agcccaaggt gcccgaactg cgccagctgg
     4561 ctgtagcgat gaatgccacg gtggggcgat tgaagtccat gtttgacgaa gaggccgacc
     4621 ggctggatgc cgtgcggcgc gaggccaact gcgatgcgtt gaccggtttg gccaatcgca
     4681 gctatttcat ggcccgtttg cgtgaaaccg ttgcggcaga tgattccagc ggtggggcag
     4741 tatttattgc gcgattggcg aatttggcga cggtcaatca aacgttgggg cgagatgcga
     4801 ccgacgagtt acttcgcgca tacggcgagg ctttggcaga agtggcacgc aagtacccgg
     4861 aggggttggc ggcgaggctc aatggtgcag actttgcact gttggtccct gcgctggcga
     4921 gcccgcagga aactgcccat cagttgttgc agtccctggt ccaggttgcg tcggcctact
     4981 tgccagagta tccgagtacc tggctcgggt gcggccattt tccgcggggc gtcgaaaccg
     5041 cggccattct ggcccaggtg gatgccgcgc ttgccgcagt ggaggccgag ggacgcaatg
     5101 ggatgcgcct ggttgatatg cgtcagggag acgaggcacc tcgaagttcg gaagaatgga
     5161 gcaacatcat ccgccgcgcc ctggagcagc ggtgggtgcg gttggtttct ttccccgtgg
     5221 catcgctcga tggaggtctt atccatcggg agtgtccgct gaggttgatg ttcgacccca
     5281 gtggtgaatg gcagcccgcc ggccgctttt tgcctgtcgc cgaacgtctg cgcctgacgc
     5341 cacaactgga tttggctgca gttgcgctgg ggctggatga actggaagcc aaacctgctt
     5401 tgcccgggct tgccatcaat ctctccgcca gctcactgga gttgacggag ttccgtacgg
     5461 aattgaggca gttactcaaa cgccgttcgg gtaccagccg cctttggctt gaggtggccg
     5521 agtccggggt gctggcccat ttcgcggctt tccgggagct ctgtagtgag ttgcgggatg
     5581 tcggctgcaa attgggcgtg gagcattttg ggcgccagtt cagcgaaatt ggccgcctcc
     5641 atgatctggg gctcgactat ctgaaggtcg acggcagctt cattcgggga ctggatcaga
     5701 acattggaaa tcagactttt ctgaaaggac tttcaaccat tgcgcacgga atcggtttgc
     5761 aggtgatcgc tgaaggagtc actagcgcgg cagagttgca ggcattgggg ctcttggggt
     5821 tcgatggctc gacgggtccc gctgtacaag acccgtcgtg acaccgacgt cgcggattta
     5881 gggcaacgct gaatgagtag ccgctggcga atgtaagcgg cacgaagatc ggtgatgcgt
     5941 ggatttcgag
//
LOCUS       DAOSLL010000256         5950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12047_length_5950_cov_3.853605, whole genome shotgun sequence.
ACCESSION   DAOSLL010000256 DAOSLL010000000
VERSION     DAOSLL010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12047_length_5950_cov_3.853605"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PLB25_11760"
     CDS             complement(<1..438)
                     /locus_tag="PLB25_11760"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOE42287.1"
                     /translation="MNTSSTAVLSVRIKRLGYLLVWVFLAGLVGCGDAGSPTMVTVDK
                     QPEHGAAAKPAAPRPKIALVMKTLTNPFFLEVEKGARRAESALDIELVVKTAAQETSI
                     EQQMLIIDDLIKARINAIVITPGDSQRLIPVLKKAAAAGIKLVN"
     gene            complement(470..3448)
                     /locus_tag="PLB25_11765"
     CDS             complement(470..3448)
                     /locus_tag="PLB25_11765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE42288.1"
                     /translation="MRFNPSPAKPEKSGQRSPRVFLTVFFGVLLVALWALIGWGIQRS
                     YANAESTARRELSNLTWAFAETVEASVNTLDIALQDLREDWSREPAKFAERVVFKQRR
                     LAKEVVFEAAIIGANGILQYSSREPQAGPIEMSDRAYFQIHNNRQQDSLFISDPVLGR
                     ISNRWSVLFTRPLLGPGGRFEGVIVLSVASDYFMRHYETFNFQEGSTSSLMDQNGHVL
                     ARWPAPDGQADGKLDLDQLMKDMDSSGQGWIEKPSAFDQVHRLHRWRKVTGHDLVVVL
                     GFSTQNMFAAQHLQRNVYLMAGVAGSVFLIWFGLLFINALRRNEQASHALHLSEERWN
                     LALESGEFTVWDLNPRTGQVFRLSRSSEIFGDELQETEASADEWASRLHPQDLPRTLE
                     LVQACLSGTQSRYATEYRVQATDGSWRWALDRGMVIGRDEAGQALRMIGTLSDITTRR
                     AEQQQLVDNQRFLRTLMDALPSMVGYWRTDMTCSFANLAHQNYFQRSAQGLQDVPLKA
                     LLGEVMFQKQHHYFEAALAGRAQMFELEWPQSDGSKHVFWTQYLPDVESDGQVHGVVS
                     LLTDVSSIKATQTTLERLNRQLLERDDKARQASQATTALQADARQDIRTLMNSVINTT
                     VQVLHSNPEYPARKELDMLEHTAETLLGVIDELESFSDIEAGRLVLEALPFKLVALVE
                     DTVAKLTASAQQKSLTLKMSVSSAIPVTVVGDPVYLGQILAQLLGNAIKFTEHGAVAM
                     EVILVSLNDKTVKLQFDVRDSGIGIELERQKSIFKPFWHKVGQTARGSHNTKGTGLGL
                     SVARALVELMEGRIWLSSRPGQGSTFSFTVKLGVPEPSSPSTGAARISNPDADITDGQ
                     TTPQPSLQSAGLDVLLVDDYPRNQTYAAGLLALMGHRVTVANNGQEAVDWVRQKDFDL
                     VLMDLQMPVMNGLEATLRIRLNETASGRPRVRIVAMTAFAVQSEVDQCFSAGMDDFVA
                     KPFTPDDLARQLDLHLNSRQ"
     gene            complement(3631..5802)
                     /locus_tag="PLB25_11770"
     CDS             complement(3631..5802)
                     /locus_tag="PLB25_11770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE42289.1"
                     /translation="MSAKSNTEAVPASQPATVMQRQSGGSGRAWGSWAWVDSSHLSSI
                     GLLERFYGIFLLVCTILLTIGVPFVFYRKLTSAIVVVALMLGILAAMRTNRRGQPEKS
                     LLWFSAFLFLVLLFLLFGGLPPVTAVTALAMTVMLTVVVDARAGWLFGGGYFLAWLSY
                     IVLTALQLAPAPYFTGSMLTSWFIGLVGVWLVMLPVPRLVQDLRQAVSLERAVIEATA
                     DGILVVNSTGGVASFNQRFMQQWQFQPDALKGAIDQDLLTSAAQQLTDPAAFLSRMRE
                     INADPAGPSFDTLTFKDGRSLEVYSNPQHLAGDIVGRVWSFRDVTERKIGEENLRQAS
                     QMAQAANLAKSRFLATMSHEIRTPMNGILGMAQLLLLPNLPDSVRNDYTRTILSSGQS
                     LLTLLNDILDLSKIEAGKFQLESVPFAPEALLQEACKLFAGAAQAKGLQLDGQWHGAS
                     GRRYLADAHRLRQMLANLIGNALKFTRTGIVRMEAKELACSDASALLEVSVSDSGIGI
                     PADKLDLLFKPFSQTDNSTTREFGGSGLGLSIVANLAQAMGGAVGVSSEPGVGSRFWF
                     RVQARPVTDTVDTRRTEREAQAAAVADSSAPLRGQVLVAEDNPVNSMVIKSLLGQLGL
                     TVKLVHDGQQAVAAVRVQVFDVILMDLQMPVLDGYQATVQIRQWEADQQRPHLPIIAL
                     TADAFDEYRQRCQAIGMDEFLTKPVALEALRVALSRWLPVS"
BASE COUNT         1301 a         1772 c         1640 g         1237 t
ORIGIN      
        1 attgaccagc ttgatgcctg cggcagcggc ctttttcagc acgggaatca agcgctgcga
       61 gtcaccgggg gtaatcacga tggcattgat ccgggctttg atcaagtcat caatgatcaa
      121 catttgctgc tcgatggagg tctcctgggc tgccgttttg accaccagtt caatgtccaa
      181 cgcactttca gcacggcgtg cacccttttc cacctcaaga aaaaaagggt ttgtcagcgt
      241 tttcatgacc agcgcgattt tgggccttgg cgctgcaggt ttcgctgctg cgccgtgctc
      301 gggctgtttg tccacagtta ccatggtcgg gctgccagcg tcaccacagc ccaccagacc
      361 cgctaaaaac acccacacca acagataccc caagcgcttg atgcgaaccg aaaggacggc
      421 agtggacgaa gtattcatgt tgctcctgcc ataaggtgga aagctggttt cattgtctgc
      481 tattcaagtg caagtcaagc tgtctggcca aatcatcagg cgtaaacggt ttggcgacaa
      541 agtcgtccat gccagccgaa aaacactgat cgacctcact ttgcacggca aaggccgtca
      601 tggcgacgat gcgtaccctg ggcctgccac tggcagtctc gttcaaccgg attcgcagcg
      661 ttgcctccag accattcatc accggcattt gcaagtccat caacaccaga tcaaagtcct
      721 tttggcgaac ccagtccacc gcctcctggc cattgttggc cacggtcaca cggtggccca
      781 tcaacgccag cagacccgcc gcgtaggtct gattccgcgg gtagtcatcc accagcaaca
      841 cgtccaggcc agcgctttgc agtgatggct gcggggtagt ctggccatct gtgatgtcgg
      901 catcagggtt cgatattctg gctgcaccag tcgaaggact ggacggctcg ggtacaccca
      961 gcttgaccgt gaacgagaag gtgctacctt gaccggggcg ggagctcaac cagattcggc
     1021 cttccatcaa ttccaccaga gcgcgtgcaa cgctcaagcc caatcccgtt cccttggtgt
     1081 tatgtgagcc gcgagcggtt tggcccacct tatgccagaa cggcttaaaa atcgactttt
     1141 gtcgttccag ttcgatgcca atgccactgt cgcgcacatc aaattgcaac ttgacggttt
     1201 tgtcgttcaa gctgaccaag atcacttcca tcgcgaccgc gccatgttcg gtaaatttga
     1261 tggcattgcc caacagttga gccaggattt gccccaggta aaccgggtca cccaccacgg
     1321 tcaccggtat ggcactgctg accgacatct tcagcgtcaa agacttctgc tgggcacttg
     1381 ccgtcagctt ggccacagtg tcttcaacca gcgcaaccag cttgaacggc aaggcttcca
     1441 gaacgagcct gccagcttca atgtctgaga aagactccag ctcatcgatc acccccagca
     1501 aggtttcggc ggtgtgctca agcatgtcaa gctccttgcg tgccgggtac tctgggttac
     1561 tgtgcaaaac ctgtaccgtt gtgttgatga cactgttcat cagggtgcga atgtcctggc
     1621 gtgcgtcggc ctgcagcgcg gttgtggctt gactggcttg ccgggctttg tcgtctcgct
     1681 caagcagttg gcggttgagc cgctcaagtg tggtttgggt tgccttgatc gaagacacat
     1741 cggttaacaa tgaaacaaca ccgtgcacct gaccgtcgct ctccacgtca ggcaaatact
     1801 gcgtccaaaa aacgtgtttg ctgccgtcac tttgcggcca ttcaagctca aacatctgtg
     1861 cgcgaccggc cagggcggct tcaaaatagt ggtgttgctt ctggaacatc acttcaccca
     1921 gaagagcttt tagcggaaca tcctgcaagc cctgcgcgct gcgctggaag taattctggt
     1981 gtgccaggtt ggcaaagctg caagtcatgt cggtgcgcca gtaacctacc atgctgggca
     2041 gggcatccat cagggtgcgc aaaaaacgct ggttgtccac caattgctgt tgctctgcgc
     2101 gccgtgtggt gatgtcactc aaggtaccaa tcatccgcag tgcctgcccc gcctcgtcgc
     2161 ggccaatcac catgccacgg tcgagcgccc agcgccagga gccgtctgtt gcctgcacac
     2221 ggtattccgt tgcatagcgg ctttgggtgc cactcaggca ggcctgcacc agttcaagtg
     2281 tgcgcggcaa gtcttgggga tgcagtcgcg acgcccattc gtccgcgctg gcttcagttt
     2341 cctgcaattc atcgccaaaa atttctgatg atcgactcaa gcgaaacacc tggccggtgc
     2401 gcgggttcag gtcccacacg gtgaactcac ccgattcaag ggccagattc cagcgttctt
     2461 cactgaggtg caacgcgtgg ctggcctgct cattgcggcg cagcgcattg ataaacaaca
     2521 ggccaaacca gatcaaaaac actgatccgg caacacctgc catcaggtaa acattgcgct
     2581 gaaggtgttg ggccgcaaac atgttttgcg tcgaaaaccc cagcaccaca accaggtcgt
     2641 gtcccgttac cttgcgccac cggtgaagcc ggtgtacctg gtcgaaagcc gatggttttt
     2701 ctatccagcc ttgcccggat gaatccatgt ccttcatgag ctgatcaagg tccagttttc
     2761 cgtcggcttg cccatcgggg gctggccaac gcgccaaaac atggccgttt tgatccatca
     2821 aacttgacgt gctgccctcc tgaaaattga aggtttcgta gtgccgcatg aagtaatccg
     2881 aggccacgga caaaacaatg acaccttcaa agcggccgcc tggaccaagg agcggccggg
     2941 taaaaagaac cgaccaacga ttggaaattc gccccagcac cggatcggag atgaataaac
     3001 tgtcttgctg acggttgttg tgaatctgaa aatacgcccg atcgctcatt tctatcggcc
     3061 cagcctgggg ctctcggctg ctgtattgca gaatcccgtt tgcgccaatg attgcggcct
     3121 cgaacaccac ttcttttgcc aggcgtcttt gcttgaacac gacgcgctca gcgaacttgg
     3181 ctggctccct tgaccaatcc tcacgcaggt cttgaagcgc aatatcaagg gtattcaccg
     3241 atgcctcgac agtttcagca aaggcccaag tcaaattgga cagttcgcgg cgcgctgttg
     3301 attccgcatt ggcatacgag cgctggatgc cccagccaat cagagcccac aaagccacca
     3361 gcagcacgcc aaaaaagacc gtcaaaaaaa cacggggcga cctttgcccg cttttttcag
     3421 gtttggcagg cgacgggttg aatctcatct gtagagaata tctcaggcta tgggctgctg
     3481 ctgccgtttt cagacgtcac cgggactgtc cgctttgtca aacccgccgc tgttcaggta
     3541 agttggtcga agtaaacatg cgagtgggtt cagtttcgcg ggcgactgct gtggcagggc
     3601 tagcagggat cagggcgatg gtgtgtgtgt tcacgaaacg ggtagccacc tggacaaggc
     3661 gactctcagg gcttcaaggg caaccggctt ggtcagaaat tcatccatac cgatggcctg
     3721 gcagcgctgg cggtattcgt caaaggcatc tgccgtcaag gcgatgatgg gcaggtgcgg
     3781 gcgttgctgg tctgcttccc attggcgtat ttgcacggtg gcctggtaac cgtccagcac
     3841 cggcatttgc aagtccatca gaatcacatc aaagacttgc acgcgcacgg cagccaccgc
     3901 ttgctggccg tcatggacca gcttgaccgt cagcccgagc tgccccagca gcgacttgat
     3961 caccatgctg ttgacaggat tgtcttcggc gaccagtacc tgtccgcgca gcggggcaga
     4021 tgaatcagcc acggcggcgg cttgcgcttc ccgctctgtc cgccgggtgt cgaccgtgtc
     4081 agtcaccggg cgcgcctgca cccgaaacca gaagcgcgag cccacgccag gctcactgct
     4141 gaccccaacc gcgccgccca tcgcctgggc aaggttggcc acgatcgaca aacccagccc
     4201 cgagccgcca aactcgcgtg tggttgagtt gtcggtttgt gaaaacggct tgaacagcaa
     4261 gtccagcttg tcggcaggaa tgccgatccc actgtcgctg accgatacct ccagcagcgc
     4321 ggatgcgtcg ctgcacgcaa gctctttggc ctccatccgg acaataccgg tgcgggtgaa
     4381 cttcagggca ttgccaatca ggttggccag catctgacgc agacggtgcg catcggccag
     4441 ataacgccgg ccagacgcgc cgtgccactg accgtcaagt tgcaaacctt tggcctgggc
     4501 ggcacccgca aaaagcttgc aggcttcctg cagcagggcc tcgggggcaa agggaacgct
     4561 ttcaagctgg aacttgccag cttcgatttt ggacaaatcc agaatgtcgt tgagcagggt
     4621 caacagggat tgaccactgg acaaaatggt gcgggtgtag tcattgcgca cgctgtcggg
     4681 caagttgggc aggagcagca actgcgccat gcccagaatg ccattcattg gggtgcggat
     4741 ttcatggctc atggtggcca gaaaacgact cttggccaga ttggcagcct gtgccatctg
     4801 gctcgcctgg cgcaaatttt cttcgccaat cttgcgctcg gtcacatcgc gaaagctcca
     4861 tacccgccca acaatgtcgc ccgccagatg ctgcgggttg gaatagacct ccagcgagcg
     4921 cccatccttg aaggtcaggg tgtcaaaact gggcccggca ggatcagcat tgatctcacg
     4981 catccggctt agaaaagcgg ccgggtcggt caattgctgc gctgccgagg tgagcaagtc
     5041 ctggtcaatg gcacccttaa gcgcatcggg ctgaaattgc cactgctgca tgaaacgctg
     5101 gttaaacgac gccacgccgc cggtgctgtt gaccaccaaa atgccatcgg ccgtggcttc
     5161 aatcacggca cgctccagcg acaccgcctg gcgcaggtct tgcaccagcc ggggcaccgg
     5221 caacatcacc agccagacac ccaccaaccc gatgaaccaa ctggtcagca tggagccggt
     5281 gaagtagggc gcgggggcca actgcagcgc agtcaacacg atgtagctca gccaggccag
     5341 aaaataccca cccccaaaca gccagcccgc gcgggcatcc accaccaccg tcagcatcac
     5401 cgtcatggcc agcgccgtca ccgcggtcac aggtggcaag cccccaaaca acaaaaaaag
     5461 cagtaccaga aaaaggaagg cggaaaacca gagcagcgat ttttccggct gcccacgccg
     5521 gttggtgcgc atcgccgcca agatacccag catcagggcc accaccacga tggccgatgt
     5581 gagcttgcgg taaaacacaa acggcacacc aatggtcaag aggatggtgc aaaccagcaa
     5641 aaaaatgcca taaaaccgct ccagcaagcc aatgctgctg agatgactgg aatcaaccca
     5701 agcccaggaa ccccaggcac gacctgagcc gccgctttgg cgctgcatga cggtggctgg
     5761 ttgactggct ggcaccgctt ctgtgtttga cttggccgac atggtggctt tgctgaggtt
     5821 tatgatgttt ttggcttcca gccattgttg tacgtgcgct ggtagctaca aacttgatag
     5881 tatttttctg gctgaagggg ttttggcatg ggcaccggtt gtcaaagctt gaagaagccc
     5941 ctggtttgtt
//
LOCUS       DAOSLL010000257         5948 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12054_length_5948_cov_4.552011, whole genome shotgun sequence.
ACCESSION   DAOSLL010000257 DAOSLL010000000
VERSION     DAOSLL010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5948
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12054_length_5948_cov_4.552011"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PLB25_11775"
     CDS             complement(<1..618)
                     /locus_tag="PLB25_11775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOE42290.1"
                     /translation="MKMIDMTGFGGAEILHLAERPVPVPGAGEMLIRVSASGVNRPDV
                     LQRMGQYPVPPGASDILGLEVAGTVELGDVAALAAAGFNPGDRVCALVAGGGYAEFCV
                     APIAQCLPIPAGLSDVEAASLPETLFTVWSNMMDRARLQAGETVLIQGGSSGIGVTAI
                     QLAKFVGARVLVTAGSDDKCAACLALGADAAINYKTQDFQAEVKRL"
     gene            complement(692..2815)
                     /gene="pnp"
                     /locus_tag="PLB25_11780"
     CDS             complement(692..2815)
                     /gene="pnp"
                     /locus_tag="PLB25_11780"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HOE42291.1"
                     /translation="MTMFKKVSKTFQWGQHTVTMETGEIARQANGAVMLDMDGTVVLA
                     TVVAKTEGKTGQDFFPLTVDYLEKFYAVGRIPGSFFKREGRASEFETLTSRLIDRPIR
                     PLFPEGFFNEVHVVVHTLSLNPEVDADIASLIAVSAALSISGVPFSGPIGAARVGYVN
                     GEYVLNPGMTARKNSAMDLVVAGTESAVLMVESEAQQLSEEVMLGAVVFGHTQGIIAI
                     NAIHELVRDAGKPMWDWKAPAKDEALIAKVGALANEKLAAAYQIRNKQARTQACRSAY
                     SEVMAGLKADGVEFDAVKVEGMLFDIEAGLVRGQILAGEPRIDGRDTRTVRAIEIRNG
                     VLPRTHGSALFTRGETQALVVTTLGTERDAQRIDALSGDYEDRFILHYNMPPFATGET
                     GRLGTPKRREIGHGRLAKRALVAVLPNKEDFPYTMRVVSEVTESNGSSSMASVCGGCM
                     SLMDAGVPLKAHVAGIAMGLIKDNNRFAVLTDILGDEDHLGDMDFKVAGTTNGITALQ
                     MDIKILGITKEIMQVALAQAKEARMHILGKMTEAMGTAKTEVSNFAPRLFTMKINPEK
                     IRDVIGKGGAVIRALTEETGTQINIDENGTITIASSDPAKAEEAKRRIEQITAEVEIG
                     KVYEGPVVKILDFGALINLLPGRDGLLHISQIAHERVEKVTDYLAEGQIVRVKVMETD
                     EKGRVKLSMKALLDRPAPSAQDNRA"
     gene            complement(3047..3313)
                     /gene="rpsO"
                     /locus_tag="PLB25_11785"
     CDS             complement(3047..3313)
                     /gene="rpsO"
                     /locus_tag="PLB25_11785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HOE42292.1"
                     /translation="MIASSIKAAVVKDNARQAGDTGSPEVQVALLTARINELMPHFKT
                     HAKDHHGRRGLLRMVSRRRKLLDYLKSKDADRYVALIAKLGLRK"
     gene            complement(3456..4733)
                     /locus_tag="PLB25_11790"
     CDS             complement(3456..4733)
                     /locus_tag="PLB25_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOE42293.1"
                     /translation="MFILFPRVLKWLALALFLPVFALAQPSVGPIKIAMIEGLSGPFA
                     NPGEAVFRNLLWATERVNARGGVRLPASAGGNQPLVLERFDSKGQNEEALTALKAAID
                     RGARIVAQGNSSAQAAALIDAINKHNEREPQKRVIFLNYSAVDPILTNEKCSFWHFRF
                     DAHADMRMSALMSVIKDDAALKSVYIIGQDYSFGQAVAREARRQLTQFRPDVRIAGDE
                     LHAVGRVKDFLPYASKIKASGAQAVITGNWGNDLTLLVKAAKEVGFDGKFYTFYGNAL
                     GAPTAMGDAGIGRVLAVADWMPNVPGAQSEAFYQAFRKRFPDPADDYVHMRMQLMMEA
                     LVQAIEAAGTTDSIAVSTQLERASVTFHSQSGSMRAVDHQFQQPLVVGVMERKGAVGV
                     KFDVEGSGYGFRVIKTIAAADAQQASTCKMQRP"
     gene            4830..5210
                     /locus_tag="PLB25_11795"
     CDS             4830..5210
                     /locus_tag="PLB25_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOE42294.1"
                     /translation="MLFTPLTPVGFRRHVYTTSRPSAQFLEPGQESRGQSATATIEQD
                     EQSFTLSFDVPGVSREQLVVGIEGNVVRLSSAEGSPRQYRFACELPQDIDATQSAAKL
                     ENGVLTLKLTKVVPVSRVTELVIH"
     gene            complement(5256..>5948)
                     /locus_tag="PLB25_11800"
     CDS             complement(5256..>5948)
                     /locus_tag="PLB25_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOE42295.1"
                     /translation="EETSPVPTFPFLCLLVSGGHTSIILLKSHFEMETLGNTIDDAAG
                     EAFDKSAKLLGLPYPGGPALAKLAQSGNPMAYKLPRPLLHSDNLDFSFAGLKTAVMVQ
                     AKKCAAAQSCLVPALPPETLADLAASTQAAIVEVLVKKSLAALKQAQMQRLVVAGGVG
                     ANRNLREQLNAECARRGVRVHYPELHLCTDNGAMIAMAAAMRLQSGQQKASEVYAFDV
                     KPRWPLDAIVGS"
BASE COUNT         1241 a         1832 c         1610 g         1265 t
ORIGIN      
        1 caggcgcttg acctcggcct gaaaatcctg ggttttgtaa ttgatggccg catctgcccc
       61 gagcgccagg caagccgcac acttgtcatc gctgcccgca gtgaccagca cccgcgcgcc
      121 gacaaacttg gccaactgga tggcggtgac cccgataccg ctggaaccac cctgaatcaa
      181 cacggtttca ccggcttgca atcgcgcccg gtccatcatg ttgctccaga ccgtgaacaa
      241 ggtttcaggt aggctggctg cctcaacatc actcaaacca gccggaatcg gcaggcactg
      301 cgcaatcggt gcgacgcaga attcggcata accaccacca gcgaccagcg cacacacccg
      361 gtctccaggg ttgaagcccg ccgccgccag tgccgccaca tcacctagct cgaccgtacc
      421 ggccacctcc agccccaaga tatccgaggc gcctggcggc accgggtatt gccccatcct
      481 ttgcagcaca tccggacgat tgacaccact ggcagacacc cgaatgagca tttcgcccgc
      541 acccggcacc ggcacaggcc gctcagccag atgcaaaatc tctgcaccgc caaaccccgt
      601 catgtcgatc atcttcatgg tcatttaagc ctctagccct tatacaatat gcgttagcag
      661 ctatcaatta tatatcaact cagcaccgtc atcaggcccg attatcctgc gcggacggtg
      721 ccggacggtc cagcagagcc ttcatcgaca gtttcacgcg gcctttttca tcggtttcca
      781 tgaccttgac ccgcacgatc tgcccctcag ccagatagtc agtgactttc tcgacgcgtt
      841 catgcgcaat ctggctgatg tgcaacaagc cgtcgcggcc aggcagcagg ttgatcaagg
      901 caccaaaatc aagaatcttg acgaccggac cttcataaac cttgccaatc tcgacttcgg
      961 cggtgatctg ctcaatgcgg cgtttggctt cctcggcctt ggccgggtcg cttgatgcaa
     1021 tggtgatggt gccgttttcg tcgatgttga tctgggtacc ggtttcttcg gtcaaggcac
     1081 gaatcaccgc gccgcccttg ccaatcacgt cgcggatctt ttccgggttg atcttcatgg
     1141 tgaacaggcg tggcgcgaaa ttggatactt cagttttggc ggtacccatt gcctcggtca
     1201 tcttgcccag tatgtgcatg cgggcttcct tggcctgggc cagtgcgacc tgcatgattt
     1261 ccttggtgat gcccagaatc ttgatgtcca tctgcaaggc agtgatgccg ttggtggtgc
     1321 cagcgacctt gaagtccatg tcacccagat gatcttcgtc gcccagaatg tcagtcagca
     1381 ccgcaaagcg gttgttgtcc ttgatcaggc ccatggcgat gcctgccaca tgcgccttca
     1441 gcggcacgcc agcgtccatc aggctcatgc aaccaccgca caccgaggcc atcgaagatg
     1501 aaccattgga ctcggtcact tcagacacca cacgcatggt gtagggaaag tcttccttat
     1561 tgggcaacac cgcaaccagc gcccgcttgg ccaggcggcc atggccaatt tcacgccgct
     1621 ttggggtgcc caggcgaccg gtttcaccgg tggcaaaggg aggcatgttg tagtgcagga
     1681 tgaaacggtc ttcatagtcg ccggacaacg cgtcaatgcg ttgtgcatcg cgctctgtgc
     1741 ccagggtggt gaccaccagc gcctgggttt cgccacgggt gaacaaggct gagccgtggg
     1801 tgcggggtaa cacgccattg cggatttcga tggcccgaac ggtgcgggtg tcgcggccgt
     1861 caatgcgcgg ttcgccagcc agaatttgac cgcgaaccag gcccgcctca atgtcaaaca
     1921 acatgccttc gaccttgaca gcatcaaact caacgccgtc ggccttgaga cctgccatga
     1981 cttccgaata tgccgagcga cacgcctgtg tgcgggcctg cttgttgcga atctgatagg
     2041 cggctgccag cttctcgttg gccagtgcac caaccttggc aatcagcgcc tcgtctttgg
     2101 ccggagcctt ccagtcccac atcggcttgc ccgcatcgcg caccagttca tggatggcgt
     2161 tgatggcaat gatgccctgg gtatggccaa acaccaccgc acccagcatg acttcttccg
     2221 acaattgctg tgcttccgac tcaaccatca gcacagccga ctcggtgcca gctaccacca
     2281 gatccatggc tgaattcttg cgcgccgtca tacccggatt aagcacatac tcgccgttga
     2341 catagcccac ccgcgcagca ccaatcgggc cgctgaacgg cacgccgctg atcgacagcg
     2401 ccgcgctgac cgcaatcaat gaggcaatgt cggcatcaac ttcggggttg agtgacaggg
     2461 tgtgcacgac cacatgcact tcgttgaaaa aaccttccgg gaacaatgga cgaatcgggc
     2521 gatcaatcag ccgactggtc agtgtttcaa actcgctggc gcggccttcg cgcttgaaga
     2581 agctgccagg aatccggcca actgcataga atttttcaag gtaatcaacc gtcaacggaa
     2641 aaaaatcctg accggtcttg ccttcggtct tggccaccac ggtggccagc accacggtgc
     2701 cgtccatgtc cagcatcaca gcgccattgg cctgacgggc aatttcgcca gtttccatcg
     2761 tcaccgtgtg ttgaccccat tgaaaggtct tgctaacttt tttgaacatc gtcatctgtt
     2821 tttcctgtcg ttctaggatt tactggaagg tgcaggccac ctcgcccgag cccggatcag
     2881 acaacacaga acacgatgcc attccagagt cacacgcccc accttggggt catgcagctt
     2941 tggaatgaca cagcttcgct ccgtttttgc catctccaac gtaaaacaag tgaaaaacgc
     3001 ctgagctagt gacctaactc aggcgtttgt cattctcttc aagcgattat ttgcgcagac
     3061 caagcttggc gatcaacgcc acataacggt cagcatcctt gcttttcaag tagtccagca
     3121 gcttgcggcg ccggctcacc atgcgcagca gaccgcggcg accatggtgg tctttggcat
     3181 gggttttgaa gtggggcatc agctcgttga tgcgggcggt gagcagtgcc acctgaactt
     3241 cggggctacc ggtgtcaccg gcttggcgtg cgttgtcttt gacaacagcg gctttgatac
     3301 tggatgcaat catgtttttt cctgtgattt aagaacttgc gtcaggcgct ggaactgccc
     3361 ctgacgtgca ctgctgctga tttcgcagag cctgtgagta tagccgggcc gacatccgag
     3421 ccgcccgggc atcaaacgca gacaccgcac agcgctcagg gacgctgcat cttgcaggtg
     3481 ctggcctgct gggcatcggc ggcggcaatg gtcttgatga cccgaaaacc ataacccgag
     3541 ccttccacgt cgaatttgac gccaactgca cccttgcgct ccatcacccc caccaccagc
     3601 ggctgctgga actggtgatc gactgcccgc atgctgccag attgtgaatg aaaagtgacg
     3661 ctagcccttt ccagttgtgt gctgacagct attgaatctg tagtgcctgc agcttcaatg
     3721 gcttgcacca gggcttccat catcaattgc atgcgcatgt gcacatagtc gtctgccggg
     3781 tctggaaagc gcttgcgaaa ggcctggtaa aaggcttcgc tctgcgcgcc tggcacgttg
     3841 ggcatccagt cggccaccgc cagcacccgg ccaatgccgg catcgcccat ggcggttggc
     3901 gcgcccagcg cgttgccgta aaaggtgtaa aacttgccgt caaaaccgac ctctttggca
     3961 gccttgacca gcaaggtcag gtcgttgccc cagttgccgg tgatgaccgc ctgcgcaccg
     4021 ctggccttga tcttgctcgc gtagggcaag aaatccttga cccgaccgac tgcgtgaagt
     4081 tcgtccccgg caatgcgcac atccgggcga aactgggtca actggcgacg tgcttcccgc
     4141 gccaccgcct ggccaaaact gtagtcctgg ccaatgatgt aaacactttt gagcgcagcg
     4201 tcgtccttta tcaccgacat caacgcactc atgcgcatgt cggcatgggc atcgaagcgg
     4261 aaatgccaga aactgcattt ttcgttggtc aaaatcggat caaccgccga gtagttcaaa
     4321 aaaatcaccc gcttttgcgg ctcgcgctca ttgtgcttgt tgatcgcatc aatcaatgcc
     4381 gcagcctgtg ccgacgagtt gccctgggcc acgatgcgcg caccccggtc aatggcagcc
     4441 ttgagggcgg tcaacgcttc ttcgttttgc cctttgctgt caaaacgctc cagcaccagt
     4501 ggctggttgc cgcccgcgct cgctggcagt ctgaccccgc cgcgcgcgtt gacccgctcg
     4561 gtggcccaca gcaggttgcg aaacactgct tcgccaggat tggcaaacgg accactcaac
     4621 ccctcgatca tggcgatctt gatcggtcct acagatggct gcgccagggc aaaaacgggc
     4681 agaaatagcg ccaaagcgag ccatttcaag accctgggaa aaagaataaa catggtgaaa
     4741 tttctcttga aaagcgtgcg agagcgcaca gattagaccc aagcggcaga ttcgttgtga
     4801 aggccgcgca gtttacaagg agttttctca tgctgtttac ccctctcacc ccggttggct
     4861 ttcgccgtca tgtttacacc accagccgtc cgtcggcgca atttctggaa ccaggtcagg
     4921 agtctcgcgg ccaaagcgcc accgcaacaa tcgagcaaga tgagcagtca ttcaccctgt
     4981 cgtttgacgt gccaggtgtc agccgcgagc aactggtggt tggcatcgaa ggcaatgtgg
     5041 tgcgcctgtc cagcgccgaa ggttcgccgc gccaataccg ttttgcctgc gagctgccgc
     5101 aagacattga tgccacgcaa agcgcagcca agctggaaaa tggcgtgctg accctgaagc
     5161 tgaccaaggt ggtgccggtc agtcgcgtca ccgagttggt gattcactga aaatgatagc
     5221 tgtcagcgca tatcccataa gggctacaac ctgatttagc taccaacaat ggcatccagc
     5281 ggccagcgcg gtttgacgtc aaaagcgtaa acctcgctgg ctttttgttg gcccgactgc
     5341 agtcgcatgg ctgcggccat ggcaatcatg gcaccgttgt cggtgcacag atgcagttcc
     5401 ggataatgca cacgcacgcc acggcgcgcg cattcggcgt tgagctgctc gcgcaggttc
     5461 cggttggcac cgaccccacc ggccaccacc agccgttgca tctgggcttg tttgagcgcc
     5521 gccagcgact ttttcaccag cacttcgaca atcgcggctt gtgttgatgc cgccagatct
     5581 gccaaggttt caggcggcag cgctggcacc aggcaacttt gggccgcagc acactttttt
     5641 gcctgcacca tcacggccgt cttgagcccg gcgaaggaaa aatcgaggtt gtcgctgtgc
     5701 agcaatgggc gcggcaactt gtaggccatc gggttgccgc tttgggccag cttcgccagg
     5761 gccggcccgc cggggtaagg caagcccagc agcttggccg atttgtcaaa cgcctcaccg
     5821 gccgcatcat caatcgtatt tccaagggtt tccatttcga aatggctttt caggaggata
     5881 atacttgtat gaccgcctga aacaagaaga cataaaaaag gaaaagtcgg gacgggtgat
     5941 gtttcttc
//
LOCUS       DAOSLL010000258         5938 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12091_length_5938_cov_4.672276, whole genome shotgun sequence.
ACCESSION   DAOSLL010000258 DAOSLL010000000
VERSION     DAOSLL010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5938)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12091_length_5938_cov_4.672276"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..768)
                     /locus_tag="PLB25_11805"
     CDS             complement(181..768)
                     /locus_tag="PLB25_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42296.1"
                     /translation="MKMSTRLIDYHSLAIMTTPTLDLVHCVFAKTIEGQQEVKTRARK
                     LPPLLRRLLILIDGKHTGRELSTYMAGQNVVEMLNELIKQDCISVVQTATPSAVIAAS
                     ADATSTPTLASPFQQPPEGRTSKELEMAQNFMINTINAEFGQHMCLSLIEAISSCEST
                     AELRQLYPSWYQTMNTSHNASKDLPGLVERLMVVL"
     gene            1289..1420
                     /locus_tag="PLB25_11810"
     CDS             1289..1420
                     /locus_tag="PLB25_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42297.1"
                     /translation="MQERDLIEEGCSFCSQQYCFDAVDVGRVFRQTNQTPPASTVVQ"
     gene            complement(1447..1767)
                     /locus_tag="PLB25_11815"
     CDS             complement(1447..1767)
                     /locus_tag="PLB25_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HOE42298.1"
                     /translation="MKQLEVKIMGQSYLLGCPDGGDARLLDAVERVDNAMCKIREAGK
                     VRARDRIAVLAALNLAFDLSDRHAQQVTAPDSASGQSAEMTQRLAALLTRLDTALNRD
                     DQLL"
     gene            complement(1764..2030)
                     /locus_tag="PLB25_11820"
     CDS             complement(1764..2030)
                     /locus_tag="PLB25_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF904 domain-containing protein"
                     /protein_id="HOE42299.1"
                     /translation="MSDLSQIDQITDRVERLLVRYEELTRTNALLYTQIDSLTQERNS
                     LKSRLSAARARVDALLERLPEDSPGSSLKAAFKSAPTTTSGDEA"
     regulatory      2066..2303
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2440..4254
                     /locus_tag="PLB25_11825"
     CDS             2440..4254
                     /locus_tag="PLB25_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HOE42300.1"
                     /translation="MAVFAAFPVLAQGQGEAALKEVVVTASRSQQLLTDALPHTTVIA
                     RDVIERSQAVDLPALLSNEAGFQFIQNGGRGTTSSLLLRGSASLGVLVLIDGVPLTRQ
                     DSTGAISLEHIMLDQVDRVEVVRGNVSAIYGSGAIGGVIQVFTRQGNGKPAGYAQLEA
                     GSYGSKRATAGISGQVGDTRFALGVGQHRTDSFSSMNAAQYPNENPDADGYRNTNYSL
                     ALSHEWRSGQTLGLRAQGNDGEFDFDGGGFGTVTDVYKGRNITDTWSLYSHNQFTEAW
                     RSELTLSHGREKSSYDARLTAYPYDSLAVTKTRTANWTNSITIGNWQATVGAEYQQQS
                     ITTADIYGPGLDRDRGVTALFAGLTATLGAHSLQANVRRDSADGLDAQSTGYLGYGYA
                     FTPAWKLIASASTAFNLPPLGYLYDPFSGNPLLKPETARSNEIGLQWAQAGQVVRATL
                     FSTRLSDMLLYDFATFSFNNVSDASNRGLEVSYSGKFAGADVRASLTQQDPVNESTGQ
                     QLIRRAKTMAAVGASLPLGAWTVGGDLRYTGARPDLATRPELGAYTVANLTARYTLSP
                     TLTLTARIDNLFDRQYQTAYGYNQPGRAIYAGLVWKQK"
     gene            4636..5268
                     /locus_tag="PLB25_11830"
     CDS             4636..5268
                     /locus_tag="PLB25_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HOE42301.1"
                     /translation="MMRQSIARSELILGGQKSGKSRRAEALAQAWLVQSGQNQAVLIA
                     TALACDDEMRLRIARHQADRLLRVPGMTTVEEPWQLAQAIRAHSRPDTLIVVDCLTLW
                     LTNWLMPFDAASAPRQPPDHQTSLNDESNQPSAPVHTAQIAMLLKAIGRVSGPLVLVG
                     NEIGLGVIPIGREVRCFVDALGNLNQDVARTCQRVTLMAAGLPLTLKDVA"
     gene            5466..>5938
                     /locus_tag="PLB25_11835"
     CDS             5466..>5938
                     /locus_tag="PLB25_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HOE42302.1"
                     /translation="MAMLLAASPAQALQVTDDRGVSISFAQPPQRIVSLLPSLTESVC
                     ELGQCARLIGVDRYSNYPDSVKKLPQVGGGLDPNIEAVVALKPDLVLLASSSRAGLRL
                     ESLGIKVLVLEPKTHADVQRVLLKLGQVLGVADAQAIWRNIDAATKAAAQSLPKGL"
BASE COUNT         1119 a         1711 c         1845 g         1263 t
ORIGIN      
        1 tagggtgcgc gaagaagaag gcttttgagt atggcctgca acatttctgg ccgcgctatg
       61 cacgcccaaa acccgattga ggaggtgaac cgaggcgttt attccgggaa gcagacttca
      121 ggggatggcg aatgactcgc gacaggattc aacctgtgct tcctcgccgt aacgaccggg
      181 tcaaagcacg accatgagtc gttcgaccaa tccaggaaga tccttgctgg cgttatgact
      241 ggtgttcatg gtctggtacc aagatgggta cagttgccgc agctccgccg ttgattcgca
      301 tgaactgatc gcttcaatca atgacaagca catgtgctgg ccaaactctg cattgatggt
      361 gttgatcatg aaattttgtg ccatttccaa ttctttggag gtgcgccctt cagggggttg
      421 ctgaaatggg ctagcgagcg tcggcgtgga cgtggcatcg gcagatgctg caatgacagc
      481 agatggtgtt gctgtctgca ccacagatat gcaatcttgt ttgatcaatt cattgagcat
      541 ttccaccaca ttctgtcccg ccatataggt tgacagctcg cgaccagtgt gtttgccgtc
      601 gatcagaata agcaaacgtc tgagtagtgg cggtagtttt cttgctcgag tctttacttc
      661 ttgttgacct tcgattgtct tggcaaagac acaatgaacc aggtcaagag tgggcgtagt
      721 catgatggcg agagagtggt agtcaatcaa tcgtgtggac attttcaaaa tggacaatgt
      781 acccttcttt gtgatttcca gggtttaccc ttgtttggaa atgaagctat gaagagggtc
      841 ctgcctggca tcacaattct ttgccatacc cactataggg agcgcggtgt gctatccaaa
      901 cttgtagctc cgcgactatt cgctttattc tgtgagggct ggcttttgtt caactttcct
      961 ttgcctgggt tgtgggtact ggatctcacc ttgatgggat tgcctttgct tgttgcggct
     1021 tggctcggat tgtgggccgg gctaattggt acgttggctt gcttgattga acggcatacg
     1081 aagactgaaa aagtccactc accatgctgc caccagtgct ggttggttgc gcttcatttg
     1141 cgcatctgct gcttctgttt ggggtggcaa gcagggggac tgacttgcgc gccaaggtcg
     1201 ttcggtcatt gccaatccat ggacatttgc gctggcgctg gggtacccct gctggccgac
     1261 cacacaccca tggcaggcgc aaggcatctt gcaagagcgc gatcttattg aagagggctg
     1321 cagtttttgc agccagcagt actgctttga tgcggtcgat gtgggccgtg tgtttcgcca
     1381 gaccaatcaa acgccacctg ccagcacggt ggtgcagtga ttaccgggaa cttctcgtca
     1441 agccaatcag agcagttggt cgtcgcggtt gagcgcggtg tccagtcggg tcagcagcgc
     1501 cgccagtcgc tgcgtcattt cggcggattg cccggacgcg ctgtcgggcg ctgtgacctg
     1561 ctgtgcatgc cggtccgaca ggtcaaatgc caggttcagc gccgccagca ctgcgatgcg
     1621 gtcacgtgcg cgcaccttgc cagcttcgcg aatcttgcac atggcattgt caacccgctc
     1681 gacagcatcg agcagccggg catcgccacc gtcagggcag cccagcaggt aactttggcc
     1741 catgattttc acttcgagct gcttcatgcc tcatcccccg aagtggtcgt tggagcggat
     1801 ttgaaagccg ctttcaggct gctgccgggg ctgtcttcag gcaatcgctc aagcagtgca
     1861 tcgacccggg cacgcgctgc gctaaggcgc gatttgagtg agttgcgctc ttgcgtcaag
     1921 ctgtcaatct gggtgtacag cagggcattg gtgcgggtga gttcttcgta gcgcaccagc
     1981 aggcgctcaa cccgatcggt gatttggtca atttgtgaca ggtctgacat aggccagcat
     2041 tgtagggttt tggcaagttg gcccggctag aatgggcagc gttggtgctc gcggtgtggt
     2101 tcacacgctg cagttcaacg ggaagcagga gggtcaggct gatgttcagc caacctaacc
     2161 tgcgctgccc ccgcaacggt aagtggacgc actgcaaggt gccgcttcca tcacggccac
     2221 tgagcgtttt gaacaagcgc ttgggaaggc gatggcggtt gattccacca gcccggatac
     2281 cggccaacac ggtggtggcg cgttttcaac gcggcaccgt gacgctcagg caccgtcggg
     2341 gacgacggtg agggcttttg atggtttctt cacccgacta tgaaaattct tgtttcctcc
     2401 cggtgcggtc gcgtacccgt gcgccttggc gcgactgcca tggctgtgtt tgccgccttc
     2461 ccggtactgg cgcaaggcca gggcgaggct gcgctgaaag aggtggtggt gactgccagc
     2521 cgttcgcagc aactgctgac cgatgccttg ccccacacca cggtgatcgc ccgtgatgtg
     2581 atcgagcgct cgcaggcggt tgatctgcct gcgctgctgt ccaacgaagc cggtttccaa
     2641 ttcatccaga acggcggccg tggcaccact tccagcctgt tgctgcgcgg cagtgcctcg
     2701 ctcggggtgc tggtgctgat cgatggtgtc ccgctgaccc ggcaagacag caccggtgcc
     2761 atcagcctgg agcacatcat gctcgatcag gttgaccggg ttgaggtggt gcgtggcaac
     2821 gtgtcggcca tttacggcag cggggccatt ggtggtgtga ttcaggtgtt cacgcgccag
     2881 ggcaacggca aaccggctgg ctatgcccag cttgaggctg gctcctatgg cagcaagcgc
     2941 gctacggcag gcatcagcgg gcaggttggc gacacccggt ttgcgttggg tgtcgggcag
     3001 catcgtaccg acagtttttc gtccatgaac gccgcgcagt atcccaatga aaacccggac
     3061 gcagatggtt atcgcaatac caactacagc ctggccctgt cgcacgaatg gcgcagcggc
     3121 cagaccctgg gcctgcgggc gcagggcaat gatggtgaat ttgactttga tggcggtggc
     3181 tttggcacgg tcaccgatgt ttacaagggg cgcaacatca ccgacacctg gtcgctctac
     3241 agccataacc agttcaccga ggcctggcgc agtgagttga ccctgagcca tggccgtgaa
     3301 aagtcaagct acgatgcccg gctgactgcc tatccctacg acagtctggc cgtgaccaag
     3361 acccgtactg ctaactggac caatagcatc accatcggca actggcaggc caccgtcggt
     3421 gccgagtacc agcagcagtc gatcaccacg gccgacatct atggtcccgg gttggaccgt
     3481 gatcgcggtg tgacggcgct gtttgccggc ctgactgcca ccttgggcgc gcattccctg
     3541 caagccaacg tgcgacgcga cagcgctgat gggctcgacg cgcagagcac cggttatctc
     3601 ggctatggct acgcgttcac gcccgcctgg aagctgattg ccagcgcatc gaccgccttc
     3661 aacttgccgc cgctgggcta tctgtatgac ccgttttcgg gcaatccgct gctcaagcca
     3721 gagacggcac gctccaatga aattggtctg caatgggcac aggcgggcca ggtggtgcgg
     3781 gccacgctgt tctcaacccg cctgtcagac atgcttttgt atgactttgc caccttcagc
     3841 ttcaacaatg tgagtgatgc ctccaaccgg gggctggaag tcagctacag cggcaagttc
     3901 gccggggccg atgtgcgcgc cagcctgacc cagcaggacc cggtcaacga gagcaccggc
     3961 cagcaactga ttcgccgggc caaaaccatg gccgctgttg gcgcatcgct gcctttgggg
     4021 gcctggacgg tgggcggtga tctacgctac accggcgcgc gcccagacct ggccacgcgc
     4081 cccgaacttg gcgcttacac cgtggccaac ctgaccgcgc gctacaccct aagcccgact
     4141 ttgacgctga cagcgcgcat cgacaacctg tttgaccgcc agtaccagac cgcctacggt
     4201 tacaaccagc ccgggcgggc aatctatgcg ggtctggtct ggaagcagaa ataaggatcc
     4261 caacacttga ccatcaacaa ctacgccatt tggccgggcg cgggtttgcc atgatcctag
     4321 attcctcagt tggacgcgcc caagtgggcc gctggcggtg cggctgggta gggcaaaagc
     4381 cgcggctcca aatgtgcttg cgcacgtttg gacggcttga accaggccag ccttgcggca
     4441 tggctgcgcc ccgcaggggc gacgacgacg cacagctccg gcccgcccgg aggcgcaacg
     4501 accccggtcg cagtgccagt ggttcaattg ggtgcgtagc tctgtgaccc aatgggtgaa
     4561 tcccatcgaa gctaaaaaag taaatgcaca taaggactta gagatgaaca caagcggtca
     4621 actgaggaag ccaggatgat gcgccagtcc attgcccgta gtgagctgat tctgggtggc
     4681 cagaaaagcg gcaagtcgcg ccgcgccgag gcgttggcgc aggcctggct ggtgcagtct
     4741 ggtcaaaacc aggcggtgtt gattgccacc gccttggcct gcgacgacga aatgcgcctg
     4801 cgcattgcgc gccaccaggc cgaccggctg ctgcgcgtgc ccggcatgac gacggtggag
     4861 gagccatggc aactggcgca ggcgattcgc gcccacagcc gcccggacac cctgatcgtg
     4921 gtagattgcc tgacgctatg gctgaccaac tggctgatgc cctttgatgc cgcatctgca
     4981 ccgcgccagc cgccagacca tcaaaccagc ctcaatgatg agtcaaatca gccttcagcc
     5041 cccgtccata ctgcgcagat agctatgctt ttaaaagcaa ttggcagagt ctccggccca
     5101 ctggtgctgg ttggcaacga gattggactg ggcgtgatcc caatcgggcg cgaggtgcgc
     5161 tgctttgtcg atgccctggg caacttgaac caggacgtgg cccgcacctg ccagcgggta
     5221 acgctgatgg cggctggttt gccgctgact ttgaaggatg tggcatgaag tgttttgttg
     5281 acagcgggtt gtcgttgccg caacggcggg tttgccaacc gcgctggctg gcctgcctgg
     5341 cggcaaaggc gaacatttcg atcttcgaga tggttcagct tggctatgcg ccaagcttgg
     5401 ccttgcgcct ggtccgatgg cgcgggcttg gcacagccgg tgcgatttgg ctgtggctgg
     5461 gcagcatggc catgttgctg gcggcaagcc cggcgcaggc gctgcaagtc accgacgacc
     5521 ggggagtgag catcagcttt gcccagccgc cgcagcgcat cgtcagcctg ctgccgtcgc
     5581 tgaccgagag cgtttgcgaa cttggccagt gcgcccggct gatcggggtg gaccgttatt
     5641 cgaactaccc tgacagcgtc aaaaagctgc cccaggtcgg cggtggcctc gaccccaata
     5701 tcgaggcggt ggtggcgctc aagcccgatc tggtgctgct ggcaagctcg tcgcgtgccg
     5761 gtttgcggct ggagtcgctg ggcatcaagg tgctggtgct cgaacccaaa acccatgccg
     5821 acgtgcagcg tgtgttgctc aagctcgggc aggtgctcgg tgtcgccgac gcgcaggcga
     5881 tctggcgcaa cattgatgcc gccaccaagg cggcggcaca gtcgctgccc aaaggtct
//
LOCUS       DAOSLL010000259         5930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12113_length_5930_cov_4.187404, whole genome shotgun sequence.
ACCESSION   DAOSLL010000259 DAOSLL010000000
VERSION     DAOSLL010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12113_length_5930_cov_4.187404"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="PLB25_11840"
     CDS             <1..480
                     /locus_tag="PLB25_11840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOE42303.1"
                     /translation="IILANAYFLPGRKIRKSLIHAAQRGVRVRLLLQGRYEYFMQYHA
                     ARALYEPLLEAGVEIFEYAASFLHAKVAVIDQSWATVGSSNMDPLSLLLAREANLVIE
                     AAPFAKDLRQELEQAMACHARPLDRLQHAGRAWWVKLLDHTALLLMRLLLFVNGKRY"
     gene            584..1285
                     /gene="folE"
                     /locus_tag="PLB25_11845"
     CDS             584..1285
                     /gene="folE"
                     /locus_tag="PLB25_11845"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HOE42304.1"
                     /translation="MNSVDPDEGTPVSVKIRARLKAARRRFHANDNIAEFIEPGELDL
                     LLDEVEAKMKGVLSSLVIDTENDHNTDQTARRVAKMYLKEVFQGRYVTAPAITEFPNA
                     EHLNELMIVGPITVRSACSHHFCPIMGKIWIGVLPNEHTNVIGLSKYARLAEWVMGRP
                     QIQEEAVVQLADLIQQKTQPDGLAIVMEASHFCMSWRGVKDVDSKMINSVMRGSFLKD
                     ANLRREFLSLIPQKG"
     gene            1288..1710
                     /locus_tag="PLB25_11850"
     CDS             1288..1710
                     /locus_tag="PLB25_11850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HOE42305.1"
                     /translation="MLVRLLYASRAVDTSPDAIESILHQSRAHNPASGVTGVLCYGGG
                     IFLQVLEGGRMAVNELYGHIQRDPRHKDVVLLHYQEITEREFAGWTMGEVNMTRINAS
                     ILLKYAEKPELDPYSVSGQVSLALLEELMATASIIGRA"
     gene            1740..2621
                     /locus_tag="PLB25_11855"
     CDS             1740..2621
                     /locus_tag="PLB25_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HOE42306.1"
                     /translation="MQARTLSRQVPAQVLLGCDFSCAPSRKKPIVLALGTLSGGRVVL
                     SKLDKFESLADWGAWLKLPQRWLGAFDLPFGLPRELVQTLGWPTSWQACLAHYCGLSR
                     EQIRHEFAAFCHARPVGGKFAHRATDRPAGSSPSMKWVNPPVAFMLHAGLPLLLQAGI
                     TLPGLHDGAASNRNDVGQPMRVALEAYPGLLAREVLGRRSYKSDDKARQTPERLIARK
                     DLITALEQGQTRLQLHLKLSHAQRDALVDDGSGDALDAVLCLMQAAWAQVRFAAGDPL
                     YGLPADLDPLEGWIITA"
     gene            complement(2660..3487)
                     /locus_tag="PLB25_11860"
     CDS             complement(2660..3487)
                     /locus_tag="PLB25_11860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MinD/ParA family protein"
                     /protein_id="HOE42307.1"
                     /translation="MSTSPTAEKELTPVPLKPLGKVLAVTSGKGGVGKTFISANLAAA
                     LAKRGQKVLVLDADLGLANLDVVLNLYPKITLHDVFTGKAKLEEAIVRAPGGFSVLLA
                     GSGMVEYSRLTPNVRDDFLHIISGLLPHYDIVLLDTGAGISDVVLFAVSLASEVLVVA
                     TPEPTSLTDAYATIKVLVGEQQRRIIRMVVNQTARMGDGRAITTQLQQVLDRFVVAKP
                     GEKVRLIHMGDIPVDPSVRQAVMRRQLLMTSMPGCPAALAISQLAGKLEDMLITRPQ"
     gene            complement(3498..4265)
                     /locus_tag="PLB25_11865"
     CDS             complement(3498..4265)
                     /locus_tag="PLB25_11865"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE42308.1"
                     /translation="MVDLRQLQLDDARALLGHASHQLPSVEIGATSYLQALIDKLCEL
                     SLKDPLTGLSNRRFFQNVLGREIEVVARSGEPALLLMLDIDFFKKVNDSYGHPAGDAV
                     LQAVAKTLSACVRPMDTVARFGGEEFVIILPSCQSSYGFQVAERIRAAVSAMIVTVAP
                     GVTIRVTISIGGAYAPRWVRSTAELWVDRADIELYRAKSEGRNRVCIEPQPILAVSAE
                     EKHLLFGPLSMDDPGWIESMVGDGSVGSSGSLMKRVN"
     gene            4448..4960
                     /locus_tag="PLB25_11870"
     CDS             4448..4960
                     /locus_tag="PLB25_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HOE42309.1"
                     /translation="MIRETATARCLMQMALAAVLITNVADAAEIFVGRVTAVPDGDTL
                     WVQPQDASAARKLRLLGIDAPEICQPGGQAARAALQALVASGTVSVEVRYRDDYGRGL
                     ARVQVGQQDIAATLVRSGHAWSNRWKHSLGPYAAEENAARLARAGLFATTNPELPRDF
                     RKRHGSCYLP"
     gene            complement(4983..5735)
                     /gene="moeB"
                     /locus_tag="PLB25_11875"
     CDS             complement(4983..5735)
                     /gene="moeB"
                     /locus_tag="PLB25_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HOE42310.1"
                     /translation="MHDADLLRYSRHILLDDIGLQGQQLLQSARVLMIGAGGLGSAAA
                     LFLGSAGVGHITVVDPDVVDLTNLQRQIAHTEARVGQPKVLSIQTAIAQINSTVRVTA
                     VGARADAVLLNELVAQADVVLDCCDNFATRHAINFACVSHRKPLVSGAAIRFDGQVCV
                     YDPRDAHSPCYACVFPPDSTVAETRCAIMGVFAPLVGLVGTLQAAEVLKLISGVGRAL
                     TGRLLMIEGRAMEFTEFRVNRSAGCPVCAAVA"
     gene            complement(5749..>5930)
                     /locus_tag="PLB25_11880"
     CDS             complement(5749..>5930)
                     /locus_tag="PLB25_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase S41"
                     /protein_id="HOE42311.1"
                     /translation="EEARKKLEDELKKPASERKTPEFGSDKDFQLIQAVKQLKGQPVV
                     VSKTQVERAEEKKEN"
BASE COUNT         1291 a         1760 c         1663 g         1216 t
ORIGIN      
        1 atcattctgg ccaatgcgta cttcctgccc ggccgcaaga tccgcaaaag cctgattcat
       61 gcggcgcagc gtggggtgcg ggtgcgcctg ctgttgcaag ggagatacga gtatttcatg
      121 caataccacg ccgcccgcgc gctgtacgag cctttgctgg aagcaggcgt tgagatattt
      181 gagtacgccg cgagctttct gcacgccaaa gtggccgtga tcgaccagag ctgggccacg
      241 gtgggctcct ccaacatgga tccgttgagc ctgctgctgg cacgcgaagc caatctggtg
      301 attgaagcag caccttttgc aaaggattta cgtcaggaac tggagcaggc catggcctgc
      361 cacgcccgtc cgctggaccg cttgcagcac gccggacgcg cctggtgggt gaagttgctg
      421 gaccatacgg ccctgctgct gatgcgcctg cttctatttg tcaatggaaa acgctattga
      481 tattatagct agatgcgcaa tatccgtaag ggctgaaggc caataaccct taaatcaaaa
      541 cgctgatacg atgtccgcct gatgtttaac cccaacccac accatgaatt cagttgaccc
      601 agacgaaggt acgccagtat ccgtcaagat ccgtgctcgc cttaaggcgg cacgcaggcg
      661 ctttcatgcc aatgacaata tcgccgagtt cattgagcca ggcgaacttg acttgctgct
      721 cgacgaagtt gaagcaaaaa tgaaaggggt gctcagcagt ctggtgattg acacggaaaa
      781 tgaccacaac acggatcaga ccgcccgccg ggtggccaaa atgtacctta aggaagtgtt
      841 tcagggtcgt tatgtcacgg cacctgccat caccgaattc cccaacgccg agcatttgaa
      901 cgaactgatg atcgtcgggc cgatcacggt acgcagcgcc tgtagccacc atttttgccc
      961 catcatgggc aaaatctgga taggcgtgct gcccaacgaa cacaccaatg tcattggctt
     1021 gtccaaatac gctcgcctgg cggaatgggt aatgggtcgg ccacaaattc aggaagaagc
     1081 cgtggttcag ttggccgacc tgatccagca aaaaacccag cccgatggcc tggcgattgt
     1141 catggaagcc agccatttct gcatgagctg gcgtggtgtc aaggatgtcg acagcaaaat
     1201 gatcaactcg gtcatgcgcg gtagtttcct caaggatgcc aacctgcgtc gcgaattcct
     1261 gtcgcttatt ccccagaaag gctaaccatg ttggttcgtt tgctttatgc cagccgtgcc
     1321 gtggacacca gtccagatgc cattgaatcg atcctgcatc agtcgcgcgc acacaatccg
     1381 gcaagcggtg tgaccggggt cttgtgctac ggcggaggca tttttttgca ggtgctcgaa
     1441 ggtggccgga tggcggtcaa cgagctttac ggtcacattc agcgcgatcc gcgccacaaa
     1501 gacgtggtat tgctgcacta ccaggaaatt accgagcgcg agtttgccgg ctggaccatg
     1561 ggcgaggtca acatgacccg catcaacgca tcgattttgc tcaagtatgc cgaaaaacca
     1621 gagcttgatc cctactctgt atcgggccag gtatcgcttg ccttgcttga ggaactgatg
     1681 gctaccgcat ccatcatcgg tcgcgcctga tttcgcgtct ggctgaagca ccccctgtgc
     1741 tgcaagctcg gactttatcc cggcaagtac cagcccaagt actgctaggt tgtgatttct
     1801 cctgtgcacc atctcgcaag aaacccattg tcttggcgct gggaaccttg tccggcgggc
     1861 gtgtcgtgct atcaaagttg gataagtttg agagtctggc cgattgggga gcgtggctga
     1921 agctgccaca gcgatggctg ggggcctttg atctgccatt tggcctgccg cgcgagttgg
     1981 tgcagaccct gggttggccc acttcctggc aggcctgcct ggcacattac tgcggcttga
     2041 gccgggagca gattcgtcat gaatttgcgg ccttttgcca tgcgcgaccg gtcgggggca
     2101 aatttgccca ccgggcaacc gatcggccag ccgggtcaag tcccagcatg aagtgggtca
     2161 atccgccagt ggcgttcatg cttcacgccg gtctgcccct gctgctgcaa gccggcatca
     2221 ctttgcccgg cttgcatgac ggcgctgcga gcaaccgcaa tgacgtgggg cagccaatgc
     2281 gggtagcgct ggaggcttat ccgggtctgc tggcacgcga ggtgcttggc cgacgcagct
     2341 acaaaagcga tgacaaggca cgccagacac ctgagcgcct gatcgcccgc aaagacctga
     2401 tcaccgcgct ggagcaaggc caaacccggc tgcaactgca tctgaaactc agccacgccc
     2461 agcgtgacgc gttggtagat gatggcagcg gcgacgcgct tgatgcggtt ttgtgcctga
     2521 tgcaggcagc ctgggcacag gttcgatttg cggcaggaga cccactttac ggattacccg
     2581 ccgaccttga cccgctggag ggctggatca tcacggcctg aacccggact gccagaaccg
     2641 gtagaaggca ggcaaaaacc tactgtggtc tggtgatcag catgtcttcg agtttgccgg
     2701 ccaattgcga gatggccagc gcggcaggac aacctggcat ggaggtcatc aacaactggc
     2761 gccgcatcac ggcttggcga actgaaggat caaccgggat gtcgcccatg tgaatcagtc
     2821 gcaccttctc gcctggcttg gccactacaa agcggtccag tacctgctgc aactgggtgg
     2881 tgatggcgcg gccatcaccc atgcgggcgg tctgattgac caccatacgg atgattctgc
     2941 gctgctgctc acccaccagc accttgatgg tggcataggc atcggtcaat gaggtcggtt
     3001 caggtgtagc caccaccagc acttcggagg ccagcgacac ggcaaacagc accacgtctg
     3061 aaataccggc accggtgtcg agcagcacga tgtcgtagtg cggcagcagt ccggaaatga
     3121 tgtgcagaaa atcgtcgcgc acgttgggag tcaggcgcga gtactcgacc atgccggaac
     3181 ccgccagcag caccgaaaac ccacctggcg cacgcacgat ggcctcttcc agcttggctt
     3241 tgccggtaaa cacgtcatgc agggtaatct ttgggtaaag gttcagcacc acatccagat
     3301 tggccaaccc caggtcagcg tccaatacca gcactttctg cccgcgcttg gccagagctg
     3361 cagccagatt ggcggagata aatgtcttgc ccaccccacc cttgccgctg gtcactgcca
     3421 acactttacc caaaggcttc aagggcaccg gggttaactc tttttctgcg gtgggggatg
     3481 tgctcacgtc actcatatca gttcactctt ttcattaaac tgcctgatga accgacagaa
     3541 ccatcaccaa ccatgctttc gatccagcct ggatcatcca tggagagcgg accaaacagc
     3601 aaatgtttct cctctgcact gaccgccaaa attggctgcg gctcaatgca cacccggttt
     3661 cggccttcag atttggcccg gtagagctcg atgtcggccc ggtcaaccca gagctcggcc
     3721 gtcgagcgga cccagcgcgg tgcataggca ccgccaatgc tgatggtgac ccgaatagtg
     3781 acgccgggtg caaccgtgac aatcatcgcg ctgacagcgg cacgaatccg ctcagccacc
     3841 tgaaacccat atgagctctg acagcttggc aggatgatga caaattcttc tccgccaaag
     3901 cgtgccactg tatccatcgg gcgtacgcaa gctgacagcg ttttggcaac cgcttgcaac
     3961 accgcatcac cggccgggtg tccgtagctg tcattgacct ttttgaaaaa gtcaatgtca
     4021 agcatcagca gcaaagccgg ttcacccgag cgcgccacca cttcaatttc gcggcccaat
     4081 acattttgaa aaaagcgccg gttggacaaa ccggtcagtg gatctttcaa agacaactca
     4141 cacagcttgt cgatcaacgc ctgcaaatac gacgtggccc cgatttcgac tgacggcagt
     4201 tggtgagaag cgtgtccaag cagcgcacgt gcatcgtcca actgcaactg ccgcaggtca
     4261 accatgagtg gtgctgactt ggagttcaca acaatgccta gcgttgaaaa ttcaagtcta
     4321 acaggttcaa atttggcctg atactcacaa caggcttgca tagtcatgcc tgacttggct
     4381 tttcctgata cgtcatcaga ctgccgcttt ctcatcagaa tttcctgctg cacgaggtaa
     4441 cccattggtg attcgagaaa ccgcgactgc acgctgtctg atgcaaatgg cgttggctgc
     4501 cgtgctgata acaaatgtgg ccgatgccgc cgagattttt gtcggtcgcg tcactgccgt
     4561 gcccgacggc gacaccctgt gggtgcagcc acaagatgcc agcgcggcaa gaaaactgcg
     4621 cctgctgggc atcgacgcac ccgaaatctg tcaacctggt ggtcaagccg cacgtgccgc
     4681 cctgcaagct ctggtggcat ctggcacggt gtcggttgaa gttcggtacc gggacgacta
     4741 tggccgaggc ctggcgcgtg tgcaggtcgg gcagcaggat atagctgcca ctctggtgcg
     4801 ctctggtcac gcttggtcaa accgctggaa acacagcctc gggccttatg ccgccgagga
     4861 aaacgccgcg cgcctggcca gggccgggct atttgccaca accaatccag agttgccacg
     4921 ggactttcgc aagcgccacg gcagttgtta cttgccttga ccgggggttt tttcgcgccc
     4981 cctcaggcca ccgctgcgca gactgggcag ccagcgctgc gattcacccg gaattcggta
     5041 aattccattg cgcgaccctc aatcatcagc aaacgaccgg tcagcgcacg cccgacaccg
     5101 ctgatcagct tgagcacctc ggctgcctgc aaagtgccaa caagccccac cagtggcgca
     5161 aaaacgccca tgatggcgca acgggtttca gccacggtgc tgtcgggcgg aaacacgcac
     5221 gcgtagcagg gcgaatgcgc atcgcgcggg tcgtaaacac agacttgacc atcaaagcgg
     5281 atggcagcac ccgacaccag cggcttgcgg tgagatacac aggcaaaatt gatggcatgg
     5341 cgggtcgcaa aattgtcgca acaatccagc accacatcgg cttgcgcgac caattcattc
     5401 aatagcacag cgtcagcacg ggccccaaca gcagtcaccc tgacggtcga gttgatctgc
     5461 gcaatggcgg tctggatgga caaaaccttg ggttgaccaa cgcgggcttc ggtatgggcg
     5521 atctggcgct gcaaattggt caggtccacc acatcagggt ccaccaccgt gatgtgaccc
     5581 accccggccg agcccagaaa caaagcggcc gctgaaccaa gcccgcccgc tccaatcatc
     5641 agcacccgtg cgctttgcag cagttgctgc ccctgcaggc caatgtcatc gagcagaata
     5701 tggcgtgagt agcgcagcag gtcagcgtca tgcatccgtg aggaaaccct agttttcttt
     5761 cttttcttcg gcgcgttcaa cctgtgtctt gctcaccacc accggctggc ccttgagctg
     5821 cttgacggcc tgaatcaatt gaaagtcctt gtccgaccca aattccggtg ttttgcgctc
     5881 ggaggccggc ttctttagct cgtcttcgag ttttttgcgg gcttcttcac
//
LOCUS       DAOSLL010000260         5912 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12165_length_5912_cov_4.410791, whole genome shotgun sequence.
ACCESSION   DAOSLL010000260 DAOSLL010000000
VERSION     DAOSLL010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5912)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5912)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5912
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12165_length_5912_cov_4.410791"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="PLB25_11885"
     CDS             complement(<1..412)
                     /locus_tag="PLB25_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42312.1"
                     /translation="MNTTTLIEPTSQPLEPGKAEYTTVLGHRVPRVDGAAKVTGSAIY
                     GDDIKLPGMLYGVCRYTDIAAGKMVSIDTAEAEKIEGVVRIATYKDITGNPVVGVIVK
                     DYLPIVKDEIVFHGDVVAVIAATTYEAACLASDKI"
     gene            622..2154
                     /locus_tag="PLB25_11890"
     CDS             622..2154
                     /locus_tag="PLB25_11890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HOE42313.1"
                     /translation="MSDLVSGRGGVHPVDEMLPTGKLATLGMQHVLVMYAGAVAVPLI
                     IGRALKLSPQEVSMLISADLFCCGLVTLIQAWGATQWFGIRLPVMMGVTFAAVAPMVA
                     MASSTPGAEGARLIFGSIIGAGLIATLIAPLVSRMLRFFPPVVTGTIITIIGISLMRV
                     GINWIFGNPFGPTAPKIVSPEHAKWLTDVASMANAAVTPASGTTVLATVVPAVPKGLS
                     LVGTVDNPKYATLLGIGVSSLVLISILLISKFGKGFVKNISVLLGIVIGAVVAVAFGL
                     MNFDNVAKAAWFDLVLPFQFGVPIFDPMLILTMTLVMLVVMIESTGMFLALSDMTGRE
                     VGQLDLARGLRTDGLGTMLGGIFNTFPYTSFSQNVGLVGVTGIKSRFVCVAAGLIMLV
                     LGLLPKLAALVEALPTVVLGGAGLVMFGMVTATGIRILSSVDFKTNRDNSFVVAVSIG
                     IGMIPLIAPNFKQWMPHGMHPLIESGILLTSISALLLNLFFNGSQDHRAESIAAARMA
                     EE"
     gene            complement(2277..3206)
                     /gene="arcC"
                     /locus_tag="PLB25_11895"
     CDS             complement(2277..3206)
                     /gene="arcC"
                     /locus_tag="PLB25_11895"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HOE42314.1"
                     /translation="MGKKIILALGGNALGDSLAEQMAAVQTSSNAIADLIEQGHELVI
                     THGNGPQVGMIEVAFEAASKTSVHSPILPMSVCVALSQGYIGYDLQNALRETLLDRGI
                     HKPVVTLITQVVVDANDPAFADPSKPIGSFFTKEEADAMIAKGAHMKEDAGRGYRQVV
                     ASPKPVDVVEKETVQALLQAGQIAITVGGGGIPVTRHGNHLKGASAVVDKDWASAKLG
                     ELIDADLLIILTAVEKVAINFGKPNQQWLDRLSVEQAREYAAQGHFAKGSMLPKVEAA
                     LSFAASRPGRQALITKLTTAKDGIEGKTGTVIG"
     gene            complement(3212..4630)
                     /gene="hydA"
                     /locus_tag="PLB25_11900"
     CDS             complement(3212..4630)
                     /gene="hydA"
                     /locus_tag="PLB25_11900"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HOE42315.1"
                     /translation="MKRLIKNGTVVSAEAALTADLLIQDGCISAVGQDLTVDGAEVID
                     AHGCYVLPGGIDVHTHFNIDVGIARSCDDFFSGTRAAACGGTTTIIDHMGFGPAGSSL
                     HHQLAVYHRAAAGQAVIDYSFHGVIQHVDEAILDEMLTLVNDEGVSSFKFYLTYGHKL
                     NDTDALRVLTRLGQIGALATVHPENDAAIAARRAQLLAAGHRAPIDHARSRPLECEAE
                     AIARMINLARLAGDAPLYIVHLSNGLGLDYLRLARAAEQPVWVETCPQYLLLDESNYL
                     REDGLKYILSPPLRARSELEKLWGGLIDGSIDTVATDHCTFPYLQRQQMAQDDFSRCP
                     NGLPGVENRMALLFSEGVMKGRISVSRYVNLTSTMPAKLFGLWPRKGSLAVGADADIV
                     VIDPRRSHTLRHSDMHDNADYSPYEGMKCQGMLVTTLSRGNVVAREGQFTGAAGAGQF
                     LARKPFDLALVQNGPVNSTFGV"
     gene            complement(4636..5832)
                     /gene="dpaL"
                     /locus_tag="PLB25_11905"
     CDS             complement(4636..5832)
                     /gene="dpaL"
                     /locus_tag="PLB25_11905"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006058.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HOE42316.1"
                     /translation="MSAFRMRMDVAENRNFTGENSPTFCRAEAQKARAFHSTIKGYRP
                     TPLLALDAFAQMLGVAKVLVKDESQRFGLNAFKMLGGSYAIARLLCERYDIDIAEFTI
                     EKFRAAGYPKATFATTTDGNHGRGVAWSARELGQHAVIYMPQGSSQERQDHITRLGAE
                     CIVTDMNYDDTVRYSLRIALERNWEVVQDTSWDGYTRIPTWIMQGYSTLADEAVEQMQ
                     AMGMARPTHVFLQAGVGALAAGVLGYLVDVFGAKNLHTVIVEPELASCLYLSGIKGEI
                     VKVGGRMATIMAGLACGEPNPIGWPLLRDCVSQFVSCADNIAALGMRVLGNPLDDDPR
                     IVSGESGAIGMGVLAAVHFAPERQALLRQFKLDKDSVVLLISTEGDTDQRGYREVVWE
                     GRYPCA"
BASE COUNT         1146 a         1735 c         1691 g         1340 t
ORIGIN      
        1 tgatcttgtc agatgccagg caagccgcct cgtaagtggt tgctgctatt accgcaacca
       61 catcgccgtg aaagacgatt tcatctttca cgatgggcaa gtaatctttg acaatgacac
      121 ccaccaccgg gttgccggtg atgtctttgt aagttgcgat gcgcaccacg ccttcgatct
      181 tctcggcttc tgcggtgtca atcgacacca ttttgccggc tgcgatgtcc gtgtagcggc
      241 aaacgccata aagcatgcca ggcagtttga tatcgtcacc ataaatggcg gagccagtca
      301 ccttggcagc accatctacc cgtggaacgc ggtggccgag aacggtggtg tactcggctt
      361 ttccaggttc aagaggctga gacgttggtt caatgagtgt tgtggtattc atgcattttt
      421 cctgtcatag gtgccaatcg caccagagtc tccagacttt ccaatgggtt gtaaagcata
      481 ttcgatgcca tcgcgccatt ggtttcgcac cggcagcggc gcagttgcca ttggctgcag
      541 ctcgccgctt gttggcacct atattgcatg aacagcctgg cgaacatttt ttggtttgca
      601 gatttttctt tggagagtgc tatgtcagac ttagtttcag gtcgtggcgg agtccacccg
      661 gtggacgaga tgctgccgac tggcaaactc gcaacactcg gaatgcagca tgtgctggtg
      721 atgtatgccg gtgcagtggc ggttcccctg attatcggcc gcgcgctgaa actcagcccg
      781 caggaagtgt ccatgctgat ttcagccgac ctgttttgct gcggcctggt caccttgatc
      841 caggcctggg gcgcaacaca gtggtttggc atccggctgc cggtgatgat gggtgtgacc
      901 tttgccgcag tggcaccgat ggtggccatg gcaagctcga ccccaggtgc cgaaggggcg
      961 cgcctgattt ttggctccat cattggtgcc ggcctgatcg cgaccctgat cgcaccgctg
     1021 gtcagccgaa tgctgcgctt ctttccgccg gtggtgactg gcaccatcat caccatcatc
     1081 ggtatcagcc tgatgcgtgt gggtatcaac tggatttttg gcaacccttt cggtcccact
     1141 gcaccgaaga tcgtcagccc ggagcacgcc aaatggctga ccgacgtggc ctccatggcc
     1201 aacgctgctg tgacaccggc ttccggcacc accgttttgg ccactgttgt tcctgctgta
     1261 cccaaagggc tgtccctggt tggcacggtt gacaacccca agtacgccac gctgctgggc
     1321 atcggtgtat cttcattggt gttgatctcg atcctgctga tttcgaaatt tggcaagggg
     1381 tttgtcaaga acatctcggt gttgctgggc atcgtgattg gtgctgtggt cgcggttgct
     1441 ttcgggctga tgaatttcga caatgttgcc aaggcagcgt ggtttgatct ggttcttccg
     1501 ttccagtttg gcgtgccgat cttcgatcca atgctgatcc tgacgatgac gctggtgatg
     1561 ctggtcgtga tgatcgagtc caccgggatg ttcctggcgc taagcgacat gaccggcagg
     1621 gaagtcggtc agttggactt ggcgcgcggt ttgcgcaccg atggtctggg caccatgctc
     1681 ggcggcattt tcaatacatt cccctatacc agtttctcgc aaaacgtcgg tctggttggt
     1741 gtcaccggca tcaagagtcg cttcgtctgt gtggcagcag gtctgatcat gctggtgctt
     1801 ggccttttgc ccaagctggc agccctggtc gaggccttgc caaccgtggt gctgggtggt
     1861 gccggactgg tgatgttcgg catggtcacg gccacgggta tccgcattct ctcaagcgtt
     1921 gatttcaaga ccaatcgcga caacagcttt gtggttgcgg tgtcgatcgg tatcggcatg
     1981 attccgctga tcgcgccaaa cttcaagcag tggatgccgc acggcatgca tccgctgatt
     2041 gagtccggca ttctgctgac ctcgatttct gcgctcttgt tgaacctgtt cttcaatggt
     2101 tcgcaagacc accgagccga gagtattgcg gcagctcgga tggcggagga ataaggatcc
     2161 tttgttgctt cgatagtcac ccaacggtga ctatcaattt gagagagtga gattgactga
     2221 tttgatagcc accgatatcc ataacgccac gatgccgccc gcagccgatc ggtcatttac
     2281 ccgatcaccg ttccggtttt gccttcaata ccgtccttgg ctgttgtcaa tttggtaatg
     2341 agtgcctggc gacccggccg ggatgctgcg aatgacaagg cggcctcgac ctttggcaac
     2401 atcgagcctt tggcaaaatg accttgcgct gcatattccc gcgcttgctc aacgctcaga
     2461 cgatctagcc actgctgatt tggcttgcca aaattgatgg ccaccttttc aactgctgtc
     2521 aggatgatca gcaagtcggc atctatcagt tcaccgagct tggcgctggc ccagtctttg
     2581 tcgacgacgg ccgacgcacc tttgaggtga ttgccgtgcc gggtcaccgg tatgccacca
     2641 ccgccaacgg tgatggcgat ttgcccagcc tgcagcaagg cctgaacggt ttccttttca
     2701 accacgtcaa ccggcttggg tgaagccacc acctggcggt aaccgcgtcc ggcatcttcc
     2761 ttcatgtgcg cgcctttggc gatcatcgcg tcggcttctt ctttggtaaa gaaggaaccg
     2821 attggcttgc tcgggtcggc gaatgccggg tcgttggcat ccaccacgac ttgtgtgatc
     2881 aaggtgacca ccggtttatg tatgccccgg tccaacaaag tttctctcag cgcattttgc
     2941 aagtcgtaac cgatgtagcc ctgactcagt gccacgcaca ccgacatcgg caggatcggg
     3001 gaatgcaccg aggtctttga tgccgcctcg aacgccacct cgatcatgcc aacctggggg
     3061 ccgttgccgt gggtgatgac tagttcatga ccctgttcga tcaggtcagc gattgcattg
     3121 gaggacgtct gcaccgcagc catttgctcg gccagggaat cgcccaaggc gtttccgccg
     3181 agcgccaaaa taatcttctt gcccatgttg ttcagacccc aaaagttgaa ttgacaggac
     3241 cgttttgaac cagcgccagg tcaaaaggct tgcgcgccaa aaattgcccg gcgcctgccg
     3301 caccagtgaa ttggccttcg cgtgcaacaa cattgccacg gctgagcgtg gtcaccagca
     3361 taccctgaca cttcatgccc tcgtaaggcg agtagtcagc gttgtcatgc atgtcgctgt
     3421 gacgcagggt atggctgcgt cgtgggtcga tgacgacgat gtcggcatcg gcaccaacgg
     3481 ccaggctgcc cttgcgtggc cacaggccaa acagctttgc tggcatcgtg ctggtcaagt
     3541 tcacatagcg cgagaccgaa attctgccct tcatcacgcc ttcggagaac agcagcgcca
     3601 tgcggttttc gacacccggc agcccgttcg gacagcgcga aaagtcgtcc tgcgccattt
     3661 gctggcgctg tagatacggg aaggtgcaat gatcggtggc caccgtgtcg atgctgccgt
     3721 cgatcaagcc gccccacagt ttttccagct cgctgcgggc gcgcagcggc ggactcagga
     3781 tgtatttcag cccgtcttcg cgcaggtagt tgctttcatc gagcagcaaa tactgtgggc
     3841 aggtctcaac ccataccggc tgctctgcag cacgcgccag tcgcaggtag tccagcccca
     3901 ggccattcga tagatggacg atgtaaagcg gtgcatcgcc agccagacgg gccagattga
     3961 tcatgcgcgc gatggcctcg gcctcgcatt cgagcggtcg actgcgcgca tggtcgattg
     4021 gcgcgcgatg gcccgcagcc agcagttgcg cgcgccgggc agcaatggcg gcatcatttt
     4081 ccgggtgtac tgtcgccaat gcgccgatct ggcccagacg tgtcaaaacc cgcaaggcat
     4141 cggtatcgtt gagcttgtgg ccgtaggtca ggtaaaactt gaagcttgag acgccctcgt
     4201 cgttcaccaa cgtgagcatt tcatccagaa tcgcctcgtc cacatgctgg atcacaccgt
     4261 gaaagctgta gtcaatcact gcctgaccag cagcggcacg gtgatagacc gccaattgat
     4321 ggtgcaggct gctaccggcg gggccaaagc ccatgtggtc gatgatggtc gtggtgccgc
     4381 cgcaggccgc cgcacgtgtg ccgctgaaaa aatcatcgca actgcgggcg atgccgacat
     4441 caatgttgaa gtgtgtgtgc acatcaatgc cgccaggcag cacatagcag ccatgcgcgt
     4501 cgatcacctc agcgccatca accgtcaggt cttgcccgac ggcgctgatg cacccgtcct
     4561 ggatcagcag atcggctgtg agcgcagctt cggcgctgac caccgtaccg tttttgatga
     4621 gtcgcttcat tggtttcagg cgcaggggta gcggccctcc caaaccacct cgcgataacc
     4681 acgctggtcg gtgtcgcctt ctgtgctgat cagcaacacg actgaatctt tatcaagctt
     4741 gaattggcgc aacagtgcct ggcgctcagg tgcgaagtga actgccgcca acacgcccat
     4801 gccaatcgca cctgactcgc ccgacacaat gcgcgggtca tcatcaagcg ggtttcccag
     4861 cacccgcatg cccaatgcag caatgttgtc agcacaagat acgaactgcg acacgcagtc
     4921 ccgcagcagg ggccagccaa tcggattggg ctcaccgcag gccagccccg ccatgatggt
     4981 ggccatgcgc ccgcccacct tgacgatttc acccttgatg ccagacaggt acaagcaact
     5041 cgccagctca ggttcaacaa tcaccgtgtg aagatttttc gcgccaaaga catcgaccag
     5101 ataaccgagc acgccggcgg ccagggcacc aacgccagct tgcaagaaca catgggttgg
     5161 ccgtgccatg cccatcgcct gcatctgctc gaccgcttcg tcggccagtg tcgaatagcc
     5221 ctgcatgatc caggttggaa tgcgcgtgta accgtcccag gaggtgtcct gaaccacttc
     5281 ccagttgcgc tcaagggcga ttctgaggct gtaacgaacc gtatcgtcat agttcatgtc
     5341 ggtcacgatg cactcggcac ccagcctggt gatatggtcc tggcgttcct gggacgagcc
     5401 ctgcggcatg tagatgacgg cgtgctgacc aagctccctt gccgaccagg caacaccacg
     5461 accgtgattg ccatcggtgg tggtggcgaa ggtggctttc gggtagccgg cggcgcggaa
     5521 cttctcaatc gtgaactcag cgatgtcgat gtcatagcgc tcgcacagca gccgcgcaat
     5581 cgcatacgag ccacccagca tcttgaaggc attgagcccg aaccgttgcg actcatcctt
     5641 gaccagtacc ttggctacac ccagcatctg cgcaaaagca tccagagcca gcagcggggt
     5701 ggggcgatag cctttgatcg tgctgtgaaa ggcccgcgcc ttctgcgcct ctgcacggca
     5761 gaacgtgggt gagttttcac ccgtgaagtt gcggttttcc gcaacatcca tcctcattct
     5821 gaaggccgac atggctggct cctgttcgtg ccgatgagct acctgacccg aggctgcgca
     5881 tcggtgagca actgagccag cacctgagcc gg
//
LOCUS       DAOSLL010000261         5896 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12202_length_5896_cov_3.699367, whole genome shotgun sequence.
ACCESSION   DAOSLL010000261 DAOSLL010000000
VERSION     DAOSLL010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5896)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5896
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12202_length_5896_cov_3.699367"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PLB25_11910"
     CDS             complement(<1..742)
                     /locus_tag="PLB25_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOE42317.1"
                     /translation="MKHHKISLTTALLLTLAPLLWAGNAVIGRLVAPLVSPMTLNLLR
                     WTLACALLLPLAAPVLRPGSSLWGSWKRFMGLSLLSIGGYNALLYLALKTSTPINVTL
                     VGSITPVWMLLIGQVFFGNAISARQWLGAVMSISGVLLVLSRGNLDVLLQVRLVAGDL
                     YILLASAAWAYYSWMLAHPTTEPPEIRRDWSAFLLAQVALGLIWSGLLAGGEWALGLG
                     KFTPSWPLAGALLFIALGPAMLAYRAWGA"
     gene            1074..2069
                     /locus_tag="PLB25_11915"
     CDS             1074..2069
                     /locus_tag="PLB25_11915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HOE42318.1"
                     /translation="MTQQTSRAIIINQHGGPEQLKLVEVSVGQPGPGEVRVRHKAVGL
                     NFIDVYQRSGLYPMSMPLQLGMEAAGLVEAVGDGVTHLQAGDRVAYASQPPGSYCDLR
                     VMPAQSVCKLPDAISFETGAAMMLKGLTAEYLLKRTQPQGGLHPGDFVLFHAAAGGVG
                     LIACQWAKALGLQLIGTAGSDAKCALAKANGAAFAINYVTEDFLARVKDITAGKGVKA
                     VYDSVGKDTWDKSLDCLSPFGLMASFGNASGPVPAFAPGILGAKGSLYVTRQTLFTHI
                     ANRQSTQAMADNLFEVVTSGQVRIHIDQHYKLEEVQQAHRDLEARKTTGCTVLTL"
     gene            2084..2701
                     /locus_tag="PLB25_11920"
     CDS             2084..2701
                     /locus_tag="PLB25_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42319.1"
                     /translation="MFINLTLLVLALLSALLLRPWRLMAGGLRLTPVLATLTVLPWLW
                     ALPSLHKMPLQLQGSGACLVLLMLGWPLAVPVLCCVGVIAALISPMPWDVALDATVWL
                     GLVPATLALGLGLVVRRWLGAHLFVYVLGRAFLGTVLSLFAASVLAQFADHRLPGIAD
                     DLSLVAHWLIAWGDAFLTGLFTAIMVAYKPEWLATWSDRLYFKHG"
     gene            2880..3368
                     /locus_tag="PLB25_11925"
     CDS             2880..3368
                     /locus_tag="PLB25_11925"
                     /inference="COORDINATES: protein motif:HMM:NF040080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6398 domain-containing protein"
                     /protein_id="HOE42320.1"
                     /translation="MNTATRVPAALKDQFDTLKLATDAFCDQCLNDEYKQLIGQALAA
                     LCRKRPSPLLTGHANSWAAGAVHALGTVNFLFDALQTPHCKAIDIWAFFGLGASTGHS
                     HSRKIRDALGMSQMQPQWTLPSLMANKPLIGMLQIKGCMVDARHLPPDAHDIAFLKAL
                     IR"
     gene            complement(3899..4732)
                     /locus_tag="PLB25_11930"
     CDS             complement(3899..4732)
                     /locus_tag="PLB25_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE42321.1"
                     /translation="MFNTTRRLALRALLLILPIALLSACSSLPGATVEAVGQRQIEHR
                     LTRQGTPVVVFENGLGATLDWWAKVVPALEADTTVLAYNRAGYGRSSPVDTTRDGQNV
                     VRELRELLQERGLQPPYVLVGHSLGGLYMQWFARRYPLEVQALVLVDSTHPAQMQGQG
                     APENWPAWVRLAFNAMTNNTAKDELKAIDATGQSVLGMPSYTVGPVEVLSALAPMQDR
                     SNPMSEHGKQLRSSLVNLYPGGRQVWVDSGHAMPLEAPEAVVSAIRRVLKPAPPLPLT
                     H"
     gene            complement(4775..>5896)
                     /locus_tag="PLB25_11935"
     CDS             complement(4775..>5896)
                     /locus_tag="PLB25_11935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE42322.1"
                     /translation="HAPAGYRFQPGRDGFGDDTSGPTPLRVYAMWTADRHYRVDITQT
                     LASRQIQYDSVMLTPASLGLLAVSLVLLWLLAVLTVQLGLRPLHRLSLAMSQRRPDDL
                     QPVATDKLDHELVPVVDNLNQTLARLRQLLDHERNALADLAHELRTPLAIMTHQIDTV
                     RQAQDEPTRQAAAARLEGVLQRTGRLVHQLLELGRLQATTEAHWGLRQLPDLVRETLA
                     AHAPLAAQRAMTLSYTGPDQLACSMPGATLELIVDNLVRNAIRHGKPGGKIEVRLSPG
                     PNSADLPPLGSDLIELTVQDDGPGLPAAEWPRLFERFHRGSQAAGQGSGLGLAIVASA
                     ARQLGGQVHCHTGLSGTGLGVTVSWPVPPPAGSANAQHR"
BASE COUNT         1122 a         1835 c         1851 g         1088 t
ORIGIN      
        1 cggcacccca ggcacgataa gccagcattg ccgggcccag tgcaatgaac agcagcgcgc
       61 ccgccagcgg ccagcttggg gtgaattttc ccaggcccag cgcccattcg ccaccggcca
      121 gcaaaccaga ccaaatcagc ccgagcgcta cctgggccag caaaaatgcc gaccagtcgc
      181 gccgaatctc gggcggctcg gtggtcggat gagccagcat ccaactgtag taagcccagg
      241 cggccgaggc cagcaagatg tacagatcac ccgctaccag ccgcacctgc aacagcacat
      301 caaggttgcc ccggctcagc accagcagca cgccgctgat cgacatgaca gcccccagcc
      361 attggcgggc cgaaatggcg ttgccgaaaa acacttggcc aatcagcagc atccacaccg
      421 gcgtgatcga gcctaccagc gtgacgttga tcggggttga agttttcagc gccaggtaaa
      481 gcagggcgtt gtagccgcca atgctgagca aggacagccc catgaagcgc ttccagctcc
      541 cccacagcga actgccgggg cgcaacaccg gcgctgccag tggcaacaac aaggcgcagg
      601 caagggtcca gcgcagcagg ttgagtgtca ttggcgatac cagcggcgcc accagacggc
      661 caatcaccgc gttgccggcc cacagcaggg gtgccaaagt cagcagcagg gcggtagtca
      721 ggctgatttt gtggtgcttc atgcgcgacc tcgccagcgc gaacaagcgg ggctgaccaa
      781 ccgcagccgt cggcaacacc cttgcacacc ctcagcaatc gcgctttgac cccggctggc
      841 ggccatgctg ccaccagcat ggcgcagtgc ctgcccttga gccgcgcgac accggttgat
      901 gcggcgacca tacgcgttgt ccgccaccgc cagcgcgggg ccttgagggt tcaattgcat
      961 tgaagaatta acaccgatca tccaggaacg tgtgttgaat gccattttgg cagcaaaaca
     1021 gcgcggccat ttttcatggc aacatgcaca ttttttccct gaaggacatt gtcatgaccc
     1081 aacagacatc tcgcgccatc atcatcaacc agcacggtgg cccggagcag ctcaagctcg
     1141 ttgaagtcag cgtaggccag ccagggccgg gcgaggtacg ggttcgtcac aaggccgtcg
     1201 gcttgaactt catcgatgtg taccagcgca gcggcctata cccgatgagc atgccgctgc
     1261 agctcggcat ggaagcagcc ggtctggtgg aggccgtggg cgacggtgtg acgcacctgc
     1321 aagcgggcga ccgcgtcgcc tacgccagtc agccgccggg cagttactgc gatttgcgcg
     1381 tgatgcctgc ccaaagcgtc tgcaagctgc ccgatgcgat ctcgtttgag accggtgccg
     1441 ccatgatgct caaaggcctg acggccgagt acctgctcaa gcgcacccag ccgcagggtg
     1501 gcttgcatcc gggtgatttt gtgctgtttc atgcggctgc aggcggtgtc ggcctgatcg
     1561 cttgccaatg ggccaaagcg ctgggtttgc agttgatcgg caccgccggc tcagatgcga
     1621 aatgcgcgct ggccaaagcc aacggagcgg cctttgccat caactatgtc accgaggact
     1681 ttttggccag ggtcaaagac atcaccgctg gcaaaggtgt caaggcggtc tatgactcgg
     1741 tcggcaagga cacctgggac aagtcgctgg actgcctgag cccgtttggc ttgatggcca
     1801 gttttggcaa tgcgtccggg ccggtgccag cctttgcacc gggcattctc ggtgccaaag
     1861 gatctctgta tgtcacgcgc cagacgctgt ttacccacat agcgaaccgc cagagcacgc
     1921 aggcgatggc cgataacctg ttcgaagtgg tcaccagtgg ccaggtcagg attcacatcg
     1981 accagcacta caaacttgaa gaagtgcagc aggcgcaccg ggacctggaa gcgcgcaaga
     2041 ccaccggctg caccgtgctg acgctgtaag gcgacctgcg ccgatgttca tcaacctgac
     2101 cctgctggtt ctggcacttc tgtcagcact gctgctgcgg ccatggcgcc tgatggcagg
     2161 cggcctgcgc ctgacaccgg tgctggcgac gttgactgtg ttgccatggc tgtgggcgct
     2221 gcccagtttg cacaagatgc cattgcaatt gcagggctcg ggtgcctgcc tggtgttgct
     2281 gatgctgggc tggccgctgg cggtgccggt gttgtgctgc gtgggtgtca ttgcggcgct
     2341 gatttcaccc atgccctggg atgttgcgct ggatgcgacg gtctggctgg ggctggtgcc
     2401 ggccaccctg gccttggggc tgggtctggt ggtgcgccgc tggcttggcg cacatctgtt
     2461 tgtttacgtg ctggggcggg cctttttggg tactgtattg agcctttttg ccgccagcgt
     2521 gttagcccag tttgccgacc accgcttgcc cggcatagca gacgatctga gtctggtggc
     2581 gcactggctg atcgcctggg gcgatgcgtt tttgaccggc ttgttcaccg ccataatggt
     2641 cgcctacaaa cccgaatggc tggcaacctg gtctgatcgg ctttacttca aacacggatg
     2701 aagcccgctc tggcagcccc aatttggcac gcttttggcc agcagccgat accctggttg
     2761 cgctggcaac gatccattga gttaatcaaa aaccatagct ggcagcgctt attcgacggg
     2821 cgccagaggc caatttctca tgtgtttcgt catctcgact gcaaccggaa cttgacacca
     2881 tgaacaccgc aacacgcgta cccgcggccc tgaaggacca gtttgacact ctgaagctgg
     2941 caaccgatgc cttttgtgac caatgcctga acgacgagta caagcaactc attggccagg
     3001 cgctggccgc actgtgccgc aagcgcccct cgccattgct cacgggccac gccaactcat
     3061 gggctgccgg tgcggtgcac gcgctgggca cagtcaattt cctgtttgat gccttgcaaa
     3121 cgccgcattg caaagccatt gacatttggg ccttcttcgg cctgggggcc agcactggcc
     3181 acagccattc cagaaaaatt cgcgacgcgc tcggcatgag ccaaatgcaa ccgcagtgga
     3241 ccctgcccag cctgatggct aacaaaccgc tgatcgggat gctgcagatc aagggttgca
     3301 tggttgacgc gcggcacctg ccgccggatg cgcatgacat cgcctttctc aaagcgctga
     3361 tccgctaggt gccgggtcgc cgggcaacgc cgagctcacg gaagtgctgc aaaatactac
     3421 caatagcata gctagcagcg catgctatac aagggctgga ggccgatttg accaaaaatc
     3481 cagaatttcg cccagcccgc catgaccgcg caagcgagct gtccggaacg tcagcaaacc
     3541 agccgccgta gcagtagcag atttggggtc ggatcccaat tcggtccaag agcgtcaaac
     3601 tgggtggcgg cggcacaccg aattgggctc tgaccccaat tctgcggacc acaccgcgca
     3661 gtcgcccagg gtgccattga ttccgcccac cgcggcggcc ggattgatgt gaataaagga
     3721 gcggtcagcg caagcaggac ggaggttgga gccagttcag gcacactggc ctgataccgg
     3781 tttaccctac ccagccgcac caggcccgct cgggcgcgtc caactgagaa atccaggatg
     3841 atccggtctg atgggcagtg aaggtttcgt catgcgtgcg aaggggccaa tgcgcaggtc
     3901 aatgggtgag cgggagtggt ggcgcaggtt tcagcacccg cctgatggcc gacaccacgg
     3961 cctcaggcgc ttcaaggggc atggcatggc cgctgtccac ccacacttgc cgaccaccgg
     4021 gatagagatt gaccagactg gaccgaagtt gcttgccgtg ctcggacatc gggtttgagc
     4081 ggtcttgcat gggcgccagg gcgctgagca cctccaccgg gccaacggtg tagctgggca
     4141 tgcccagcac cgattggccg gtggcatcaa tggctttcaa ttcgtctttg gcggtgttgt
     4201 tcgtcatcgc gttgaaagcc agacgaaccc aggcaggcca gttttctggg gcaccttgcc
     4261 cttgcatctg tgccgggtgg gtcgagtcca ccagcaccag ggcctgaact tccagcgggt
     4321 aacgtcgggc aaaccactgc atgtacaacc cccccagcga gtgccccacc agcacatagg
     4381 gcggttgcag gccacgctct tgcagcagct cacgcagttc gcgcaccacg ttctgtccat
     4441 cgcgggtggt gtcaaccggg ctgctgcggc catacccggc ccggttatag gccagcactg
     4501 tggtgtctgc ttcgagtgct ggcaccacct tggcccacca gtccaaagta gcgcccagtc
     4561 cgttttcaaa gaccacgacc ggggtgccct ggcgggtcaa tcggtgttcg atttgacgtt
     4621 gtcccaccgc ttcaacggtg gctcctggca acgagctgca agctgacagc agcgctattg
     4681 gcaaaatcag caacagggcg cgcaaggcca gtcggcgagt agtgttgaac atagggaaat
     4741 gggtgcatcg gtgttgagac cgaggcatgg tatgtcagcg atgctgagca ttggctgagc
     4801 ctgctggcgg cggtaccggc catgagacag tcacgcccag cccggtgccg ctgagcccgg
     4861 tgtggcagtg aacctggccg cccaactgcc gtgccgccga tgccacgatt gccaaaccca
     4921 ggccagagcc ctgacccgca gcctgcgagc cccggtggaa acgctcgaac agccttggcc
     4981 attcggcggc tggcaggcct ggcccgtcgt cctgcaccgt cagctcgatc aagtcgctac
     5041 ccagtggcgg caggtcagca ctgttggggc cgggcgacag gcgcacttca atctttccgc
     5101 caggtttgcc gtgccgaatg gcattgcgca ccaggttgtc cacgatcagt tccagcgtgg
     5161 caccaggcat gctgcaggca agctggtccg gtccggtgta cgacagtgtc atggcgcgct
     5221 gcgctgccag aggggcgtgc gcggcgagcg tttcgcgcac cagatcgggc agttgccgca
     5281 agccccagtg ggcttcggtg gtggcttgaa ggcggcccag ttccagcagt tggtgcacca
     5341 gccggccggt gcgctgcaaa acgccctcca gccgggcggc tgccgcttgt cgggtcggtt
     5401 cgtcctgggc ctggcgcacc gtgtcgatct gatgcgtcat gatggccagc ggggttcgca
     5461 attcgtgcgc caggtcggcc agggcattgc gctcatggtc gagcaactgc cgaagccggg
     5521 ccagcgtctg gttcaggttg tcaaccaccg gaaccagttc gtggtccagc ttgtcggtgg
     5581 ccaccggctg caggtcgtcg gggcggcgct gggacatcgc tagcgacagc cggtgcaagg
     5641 ggcgcaagcc caactgcacg gtgagcacgg ccagcagcca taacagcacc aggctgaccg
     5701 ccagcaaacc caggctggcc ggggtgagca tcacactgtc gtactggatt tgccgacttg
     5761 ccagggtttg cgtgatgtcc acccggtagt gccggtctgc tgtccacatg gcgtacaccc
     5821 gcagcggggt ggggccactg gtgtcgtcgc caaagccatc gcggccgggt tgaaagcggt
     5881 agcctgcggg cgcatg
//
LOCUS       DAOSLL010000262         5894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12210_length_5894_cov_4.501113, whole genome shotgun sequence.
ACCESSION   DAOSLL010000262 DAOSLL010000000
VERSION     DAOSLL010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12210_length_5894_cov_4.501113"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="PLB25_11940"
     CDS             <1..331
                     /locus_tag="PLB25_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HOE42323.1"
                     /translation="WLDQIAPQHVTEIHLAGHCHVLPQPGEDALAEIVVDDHGSRVCA
                     EVWQIYQHAISRFGQLPTLIEWDTDVPALDVLLAEAARARASARAGRPTPDSRQTRLA
                     RGAGVTA"
     gene            328..1107
                     /locus_tag="PLB25_11945"
     CDS             328..1107
                     /locus_tag="PLB25_11945"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HOE42324.1"
                     /translation="MSTLAQQQQALLAVLFDWPNQNAISNIAAYADSTWARGQKVYQS
                     NGHALCSSALRAAYPVLTQLVGDESFEALARALWHASPPLHGDAAQWGGGLAQFVRAS
                     EQLADEPYLADVATLEWALHCAASAADGQVDAASFALLAEHDPANVYLQLSPGSATLC
                     SAWPVVSIVNAHLQQNLGLVQVGQLLRDRAGQDALVWRSGLQPLVRQAQAGEAAFAAA
                     LLRGQSLGDALDQAGALDISTWLPMAVQTGLLLGVRLTSFA"
     gene            complement(1314..2099)
                     /locus_tag="PLB25_11950"
     CDS             complement(1314..2099)
                     /locus_tag="PLB25_11950"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HOE42325.1"
                     /translation="MTSLQNFQAQFQTALLVDQPPLPGLFDQLGEAQFAVYLHAYRAR
                     LRYALRDNYESVAQIMGDDAFDALANAYIDAHPSTHYSVRWFGHQLSHFMAANEALIA
                     HPAMIDLAVMEWALRSAFDAPQAGRLEPAELAALAIDDWPQLQFELHPAVQLLHLQWA
                     VGPVWHALKAGQLEVEAPNALAHPMLIWRDGLHTQWKSLSVAEHVFVEGVLAGDTFAQ
                     LCDALANEVGEDHAAQTAVMVLRELMLTGVISSLHMAAPVSEP"
     gene            complement(2096..2935)
                     /locus_tag="PLB25_11955"
     CDS             complement(2096..2935)
                     /locus_tag="PLB25_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HOE42326.1"
                     /translation="MTSIQGFGVGLRTEHYTAFESGAQPRVDWLEILSENYMVPGGAP
                     LWHLDRIRQDFPVVMHGVSLNIGSTDPLNPDYLRDLKTLIQRVQPGWVSDHLCWTGVN
                     GLNMHDLLPLPYTQAMLRHVSDRIQRVQDYLGQRLVIENPSTYVAFAANEMPEWVFLG
                     ELLRLADCELLLDVNNVYVSSVNHRFDPHIYIDAIPSARVRQIHLAGHETHDGYLIDT
                     HDHPVCEAVWQLYAFTLRHCGSVPTMIERDDNIPELHELLDELDLARQIHAQTWIAEE
                     SLA"
     gene            complement(3004..3264)
                     /locus_tag="PLB25_11960"
     CDS             complement(3004..3264)
                     /locus_tag="PLB25_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011830415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42327.1"
                     /translation="MSSKFKSAVALASTAAALFAISAPLTTAHAAGDMSVKCAGLNSC
                     KGTSDCKTAKSECKGHNSCKGQGWNAKATAQECTSAGGTVLK"
     gene            complement(3428..3922)
                     /gene="msrB"
                     /locus_tag="PLB25_11965"
     CDS             complement(3428..3922)
                     /gene="msrB"
                     /locus_tag="PLB25_11965"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008251033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HOE42328.1"
                     /translation="MRFSVTRRLLLSMLASLGMFRSAQATPDASGTSRPDPWRLTDAE
                     WKRRLTPAQYDVLRHEGTERAHTSALNAEKRTGVYHCAGCDLPLFGSDTKFDSGTGWP
                     SFFAPLPGAVATKTDFKLVFPRTEYHCARCEGHQGHVFSDGPAPTGKRYCNNGVALRF
                     ASAG"
     gene            4292..4588
                     /locus_tag="PLB25_11970"
     CDS             4292..4588
                     /locus_tag="PLB25_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42329.1"
                     /translation="MINGHEFKDLPSSQIEPGLADQAHYLASEPTMQRLLRHLHQNIH
                     RRRKRRAKKRHKPFALNANVVNLDCTWDITHLLTMTKGQHFYPFADRLHQPGVD"
     gene            complement(4629..>5894)
                     /locus_tag="PLB25_11975"
     CDS             complement(4629..>5894)
                     /locus_tag="PLB25_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE42330.1"
                     /translation="SDGRAVLSQVAQARGKYEPLQAEAMELAIGGQTFEAKALLLDKV
                     RPAQKVYFDALDSLLQHQNALMQQSASATQSATANMVYVIGLTLLLALGVGVLMALWI
                     IRSITRPINLAVQVSRAVAAGDLSVQFDTSGKSEIAQLLLALKEMQTSLVNVVSDVRD
                     GAKGVAVASAGIAQSDMDLSSRTENQSHSLRDTTASMEQLGTNVNLNADNARKANQLA
                     QSASEVATRGGEVVTQVVDTMKGINESSRKISDIISVIDGIAFQTNILALNAAVEAAR
                     AGEQGRGFAVVASEVRSLAGRSAEAAREIKALISASVQRVEHGTLLVDQAGTTMSEVV
                     SSIRHVTDIMGEISVASTAQSEGVSQLGVAIHLMDQMTQQNAQLVEVMSEAAGSLQAQ
                     ADALVNTVAVFKLGDHGRPGRLALQVQNA"
BASE COUNT         1181 a         1729 c         1756 g         1228 t
ORIGIN      
        1 ctggctagac caaatcgcgc cgcagcatgt gaccgaaatc catctggccg ggcattgcca
       61 tgtgctacca cagccgggcg aagacgcgct ggccgagatc gtcgtcgatg accacggcag
      121 ccgcgtttgt gccgaggtct ggcagattta ccagcacgcc atcagccgct ttggccagtt
      181 gccaacgctg atcgagtggg acaccgacgt gcccgcgctg gatgtcctgc tggctgaggc
      241 cgcgcgcgcg cgcgcctcag cgcgggctgg tcgccccacc ccggatagcc ggcagacccg
      301 gttggcgcgc ggcgctggcg tgacagcatg agcactttgg cgcaacagca gcaagccctg
      361 ctggccgtgt tgtttgactg gccaaatcaa aatgctataa gtaacatagc tgcttacgca
      421 gacagcacat gggctagagg ccaaaaagta tatcaatcca acgggcacgc gctttgcagc
      481 agtgcgctgc gcgcggccta tccggtgctg acgcaactgg tcggcgatga gagttttgag
      541 gccctggcac gcgccttgtg gcacgccagc ccaccgctgc acggtgatgc agcgcaatgg
      601 ggtggcgggc tggcgcaatt tgtccgtgcc agcgagcaac tggccgatga gccttatctg
      661 gccgatgtgg caacgctgga atgggcgctg cactgcgctg ccagcgccgc tgacggccag
      721 gttgatgcag ccagttttgc actgctggcc gaacacgacc cggccaatgt gtacttgcag
      781 ctttcgcctg gcagcgccac cctgtgcagc gcttggccgg tggtcagcat cgttaacgcg
      841 catctgcaac aaaacctcgg gctggtgcaa gttggccagt tgttgcgcga ccgtgcgggc
      901 caggatgcgc tggtctggcg cagcggcttg cagcccttgg tgcgccaggc gcaagccggt
      961 gaggcggcat ttgcagccgc cttgctgcgc gggcaatctc tgggtgatgc gctggaccag
     1021 gctggcgcgc ttgacatcag cacctggctg ccgatggcgg ttcagaccgg gttgctgctg
     1081 ggtgtgcggt tgacttcgtt tgcctgaatg ccttatgaca aatagaccgc tagcccttgt
     1141 ctgtcatgtg ctgatagcta tttaatttgt agttagcgag ggtacttgga tgcgccagac
     1201 cgtcgtcgcg cagagccgac gattgctggt gcctggcttg gcgcctggtt aacgtgaaac
     1261 gactgtcagg tccggggtgg ctggcgtcca tgaaagtttg gctgggagct ctttcagggt
     1321 tcggacacag gtgccgccat gtgcaggctg ctgatcacgc cggtcagcat caattcgcgc
     1381 aacaccatta cagccgtctg ggcggcatgg tcttcaccga cctcgttggc caatgcatca
     1441 caaagctgcg caaaggtgtc gccggcaagc acaccctcta caaatacatg ttcggctact
     1501 gacagtgact tccactgggt gtgcaagcca tcgcgccaga tcagcatcgg gtgcgccagc
     1561 gcgttcgggg cttcaacctc aagctgaccc gccttgagtg cgtgccagac tggtcccacc
     1621 gcccactgca aatgcagcaa ttgcactgca ggatgcagct caaactgaag ttgcggccag
     1681 tcatctatgg ccaatgcagc cagctcagcg ggttccaggc gacccgcctg cggggcgtca
     1741 aaggcgctgc gcaaggccca ttccatcacg gccagatcaa tcatcgccgg gtgcgcaatg
     1801 agggcctcat tggcagccat gaagtggctc aactgatgac caaaccagcg caccgaatag
     1861 tgggtggagg gatgggcatc aatgtaggcg ttggccaatg catcaaacgc atcatcgccc
     1921 atgatctgcg ccacagattc gtagttgtca cgcagcgcat agcgcaggcg tgcacgatag
     1981 gcatggaggt agacggcaaa ttgtgcttcg ccaagttggt cgaacaagcc aggcaaaggg
     2041 ggttggtcca ccagcagcgc tgtctgaaac tgcgcctgga aattttggag agaggtcatg
     2101 cgaggctttc ctcagcgatc catgtttgtg catgaatctg gcgcgccagg tctaactcgt
     2161 cgagcaattc atgcagctcg gggatgttgt cgtcgcgctc gatcatggtg ggcaccgaac
     2221 cgcagtgccg cagcgtgaaa gcatagagct gccataccgc ttcgcaaacc gggtgatcgt
     2281 gggtgtcgat cagatagcca tcgtgcgttt catgcccggc caggtgaatc tggcgcaccc
     2341 gcgccgaagg tatggcatcg atataaatat gcgggtcaaa ccgatggtta acgctgctga
     2401 catagacgtt gttcacatcc agcagcaact cgcaatcggc caaccgcagc aactcgccca
     2461 agaaaaccca ttcgggcatt tcgttggcgg caaacgccac ataggtcgat gggttttcaa
     2521 tcaccaggcg ttgacccaaa taatcctgta cccgctggat gcgatcactc acatggcgca
     2581 gcatggcctg ggtatatggc aggggcaaca agtcatgcat gttcaggcca ttgacaccgg
     2641 tccagcacaa atgatcagac acccagccgg gctgcacgcg ctgaatcaag gttttgagat
     2701 cgcgcaggta gtccgggttg agcgggtcgg tgctgccaat attgagggac acaccatgca
     2761 tcaccacggg gaagtcctgg cggatacggt ccagatgcca cagtggcgcg ccgccgggca
     2821 ccatgtagtt ttcagacaga atttccagcc aatcgacgcg tggctgggct ccggattcaa
     2881 acgctgtgta gtgctcggtg cgtaagccca caccaaaacc ctggattgat gtcatgggag
     2941 tcaatggctt tgttgcgata cctgccctac aacgggtagg gcctggtttc gcgacaagtc
     3001 tgcttacttg aggaccgttc cgcctgcaga ggtgcattct tgcgcagtgg ccttggcgtt
     3061 ccagccctgc cccttgcagc tattgtgtcc cttgcattcg ctcttggcgg ttttgcagtc
     3121 ggaagtcccc ttgcaactgt tcaggccagc gcatttgacc gacatgtcac cggcggcatg
     3181 ggccgtggtg agaggtgcgc tgatggcgaa cagggcagca gcggtactgg ccagggcgac
     3241 ggcggatttg aatttggatg acatggatgt tcctttgaag gtgagtgaga agataaaaag
     3301 acaaaacaag acggaattgc aaaaagggct tgagccttgg gtgctttgac tcctgcttgc
     3361 tcttgtcagt cggggcagaa ttcaaaaact tacacaaaaa accatcattg accaagaaaa
     3421 atttcgctca ccctgcactt gcaaacctga gcgcgacgcc gttgttgcaa tagcgctttc
     3481 cggtcggtgc cgggccatcg ctgaacacat gaccttgatg cccctcgcag cgcgcgcagt
     3541 ggtattcggt gcgtgggaaa accagtttga aatcggtttt tgtcgccacg gctccaggca
     3601 atggggcgaa aaaactgggc cagccggtgc cgctgtcaaa cttcgtgtct gacccaaaca
     3661 gcggcaaatc acagcccgca cagtggtaaa caccagtgcg cttttcggca ttaagcgcac
     3721 tggtgtgggc gcgctcggtg ccctcatggc gcagcacatc gtactgcgcc ggtgtcaggc
     3781 gccttttcca ctcggcatcg gtcaaacgcc aggggtctgg gcgtgatgtt ccgctggcat
     3841 caggcgtggc ctgggctgat ctgaacatac ccaagctggc aagcatggaa agaagtaggc
     3901 gtcgtgtcac ggaaaacctc atagagagtg gttgatacag gcttggtcgt gcaacgtggc
     3961 caaatctttc aagcacgcct tgattcgtcc atcaatccct cgggttaaag cgacatccat
     4021 ggcccagagt gccaacctct tttgggtgtc gaaatgacaa aaccgccagt gagccgctgg
     4081 catattggtg caatgtgcga gctacgccgc gccgtggcta gctgaaaaaa tcatctggtc
     4141 aactcgtttg gtgcttcgag aagccggttc acatccgatt cggacatgcc caactgcgtt
     4201 gcaaatcgaa gtggattgaa gcgctggctg ctgaagatag ctcttgactt gatgtcggcg
     4261 acaaagttgc cagtgagccc tgctgctggc ggtgatcaac ggccatgagt tcaaggactt
     4321 gccatccagc cagatcgagc ctggtctggc tgaccaagct cactatttgg caagtgagcc
     4381 gaccatgcag cgcttgctgc gccacttgca tcagaacatc caccgacgca ggaagcgccg
     4441 agccaagaag cgccacaaac cctttgcgct caacgccaac gtggtcaatc tggactgcac
     4501 ctgggacatc acgcatttgc taacgatgac caaggggcag cacttctatc cgtttgctga
     4561 ccggctgcac caacccggtg ttgattgaca cctgcggcaa ttggcctgga tgcgctggct
     4621 acttcgcttc aggcgttctg cacctgcaat gccagtctgc caggcctgcc atggtcgccc
     4681 agcttgaaca ccgccacggt gttgaccagc gcgtcggctt gcgcttgcag gctgccggcg
     4741 gcttcggaca tcacttcgac cagttgcgca ttctgttgcg tcatttggtc catcaggtgg
     4801 attgccaccc cgagctgcga cacaccctcg ctctgcgcag tgctggccac actgatttcg
     4861 cccatgatat cggtgacgtg gcgaatgctg ctgaccacct cggacatggt ggtgcctgcc
     4921 tggtccacca gcagcgtgcc gtgttcgacc cgttgcacgc tggcgctgat caaggccttt
     4981 atttcgcgtg ctgcctcggc agagcgccct gccaggctgc gcacctcgct ggccaccacc
     5041 gcaaaacccc ggccttgttc gcccgctcgt gccgcttcga ccgccgcatt gagcgccagg
     5101 atattggttt gaaaagcgat gccgtcaatc acgctgatga tgtcggatat ctttctggat
     5161 gactcgttga tgcctttcat ggtgtcaacc acctgggtca ccacttcacc gccacgcgtt
     5221 gccacctcgg acgcgctctg ggccagttga ttggccttgc gggcgttatc ggcgttgagg
     5281 ttgacattgg tgcccaactg ctccatggag gccgtggtat ccctgagcga atgcgactgg
     5341 ttttcggtgc gcgaggacag gtccatgtca ctttgcgcaa tgccggcact ggcaacggcg
     5401 actcctttgg cgccatcgcg cacgtccgac accacattga ccaggctggt ctgcatctct
     5461 ttgagtgcca gcagcaactg cgcgatctcg cttttgccgc tggtatcaaa ctgcacgctc
     5521 aggtcacccg cagcaacggc ccgcgacacc tgcacggcca gattgatcgg ccgggtgatg
     5581 gaccggatga tccacagcgc catcaaaaca cccaccccga gggccagcag cagggtcagc
     5641 ccgatcacat aaaccatgtt ggcggtggca gactgcgtgg cgctggccga ttgctgcatc
     5701 agcgcatttt ggtgttgcag caggctgtcc agcgcatcaa aataaacctt ctgtgctggc
     5761 cgcaccttgt caagtagcag cgctttggcc tcaaaagttt ggccaccgat ggcaagttcc
     5821 atggcctcgg cctgaagggg ttcgtatttg ccccgggctt gtgccacctg gctcagcacg
     5881 gcccgaccat cgct
//
LOCUS       DAOSLL010000263         5870 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12273_length_5870_cov_4.658985, whole genome shotgun sequence.
ACCESSION   DAOSLL010000263 DAOSLL010000000
VERSION     DAOSLL010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5870
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12273_length_5870_cov_4.658985"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            63..1928
                     /gene="hscA"
                     /locus_tag="PLB25_11980"
     CDS             63..1928
                     /gene="hscA"
                     /locus_tag="PLB25_11980"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HOE42331.1"
                     /translation="MALLQISEPGQSPNPHQRRIAVGIDLGTTHSLVAAVRNSVAECL
                     PDAQGRAILPSVVRYLAGGGRHIGFQAIESGVGDPENTIASVKRFMGRSLKDVTLAGK
                     LPYRFVDHPGMLGLQTVQGEKSPVEISADILATLRYRAEDSFDDELYGAVITVPAYFD
                     DAQRQATKDAAQLAGLNVLRLINEPTAAAIAYGLDNASEGVYAIYDLGGGTFDISILR
                     LTQGVFEVVATGGDSALGGDDYDHALADWVLAQLGMGRMEDASAVDKSALKAAARACK
                     EALSATDSVAFSAVLTGATVSFDVEKADFEAASQALTARTLTAVRKTLRDAGLASDAV
                     KGVVLVGGATRMPQIRQAVARFFGQLPLTNLNPDEVVALGAAIQANQLAGNNPGGDLL
                     LLDVIPLSLGVETMGGLVERIVLRNETIPTAKAQDFTTYIDGQTALALHVVQGERDLV
                     ADCRSLARFELRGIPPMVAGAARIRVTFTVDADGLLSVSALEQGSGVEAHIAVKPSYG
                     LADDQIARMLAESFSTAEADMLARAVTEARVDADRMVLATQSALAADGDLLDEQQREE
                     VDQLVRELQAARSLSDAAAIEAVTKALAQGTEAFAALRMNRGIQKALSGKNIASI"
     gene            1997..2335
                     /gene="fdx"
                     /locus_tag="PLB25_11985"
     CDS             1997..2335
                     /gene="fdx"
                     /locus_tag="PLB25_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HOE42332.1"
                     /translation="MPTIKILPHAEYCPQGAEVSAPAGTSICEALLEHHINIEHACEM
                     SCACTTCHVIVREGFDTLAASEEEEDDLLDRAWGLQPQSRLSCQAILAQHNLVIEIPK
                     YSINHAKENH"
     gene            2389..3105
                     /gene="dnaQ"
                     /locus_tag="PLB25_11990"
     CDS             2389..3105
                     /gene="dnaQ"
                     /locus_tag="PLB25_11990"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HOE42333.1"
                     /translation="MRQIFLDTETTGLSADNGDRVIEIGCVELVNRKLTGNNRHFYLN
                     PGRDSHEEALKVHGLTSEFLKDKPRFEAVAADLLDYLKDAELVIHNAPFDLGFLNMEL
                     ALIGLPPLRGQVANVIDTLVMAKELYPGKRNSLDALCDRLEVDNSGRALHGALLDAEL
                     LADVFINLTRGQDALLMDDSSATDSAGLATAALDLSSFILTVLPANQQELAAHEDVLK
                     QIDKSSGGKTVWRKLADAAV"
     gene            complement(3424..3834)
                     /locus_tag="PLB25_11995"
     CDS             complement(3424..3834)
                     /locus_tag="PLB25_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42334.1"
                     /translation="MTRIALALSMATAMLAACSVNNGAPGDLREHLQTRGIKLQEIDS
                     WAPISGRSGYILAAHHPATVVALVAAFGLQPVTEASHRQRCLASVPAKPVEVWQVANR
                     PAALKLADGGQFEYLCVVIVPASQMYLVVEYAYG"
     gene            complement(3930..4205)
                     /locus_tag="PLB25_12000"
     CDS             complement(3930..4205)
                     /locus_tag="PLB25_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2164 domain-containing protein"
                     /protein_id="HOE42335.1"
                     /translation="MGIELSKPDREQAVASIERYFREHFDEKIGNIAASGLLAYFLED
                     IGPSIYNRAVADVQARLQARVAEVDFEVHEDEFAYWRKFDKQRKARK"
     gene            4341..5798
                     /locus_tag="PLB25_12005"
     CDS             4341..5798
                     /locus_tag="PLB25_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42336.1"
                     /translation="MHDRPFSSLAIADTRLSVLDDCTPMAPPDPGAATLFMQSSVVLQ
                     AGQLEAAEALLRDALQLAPHLAEAHANLAWLLERRGLPEDALQHYAMASELQPGNARI
                     QVNFGAFLAGRQFHNAAEVAYRLALLADPAMPGAWCNLGALQAQTFRDQDAEQSLRTA
                     LQLDARHVAAHVNLAFLLLRLGRFHEGWQHLEWRDWYRAIETRLDCPRWQGQPLAGCP
                     VLLVYEAGHGDMIQFCRYLPLVRSRGASRVDVLCHPALKVLLQSLDGVGEVIGLDQEA
                     SGRTWHYWVPLLSLPCHFGTRLDTIVANIPYLQAPADRFDHWRAELLRVCPGPVLRVG
                     LVWQGNADFENDAHRSLPDLGILAPLWAVVGVQFFSLQKGPGQRDVLRHQASQPLLDL
                     ADGLQDFADTAAVIGQLDLVISVDTAVAHLAGALGKPCWVLLPAVMTDWRWLEQRTDS
                     PWYPEVLRLFRQPRQVGWAPVIEQLRVALTSEVNA"
BASE COUNT         1111 a         1772 c         1839 g         1148 t
ORIGIN      
        1 gccctaccca gacgcgccgc cagcggccca ctcgggcgcg tccaactgag gaatcaaggt
       61 ttatggcatt actccaaatc tcggaaccgg gccagtcgcc caacccacac cagcggcgca
      121 ttgccgtcgg cattgacctg ggcaccaccc attcgctggt ggctgcggtg cgcaacagtg
      181 ttgcagagtg cctgcctgat gcgcaggggc gggcgatttt gccgtcggtg gtgcgttacc
      241 tggccggcgg tggccgccat attggctttc aggcgatcga gtccggcgtg ggcgacccgg
      301 aaaacacgat tgcgtcggtc aaacgtttca tggggcgcag cctcaaggat gtgacgctgg
      361 cgggcaaact gccctaccgg tttgttgacc atcccggcat gctgggcctg caaaccgtgc
      421 agggcgaaaa gtccccggtg gaaatcagcg ccgacatcct ggccaccctg cgctaccgcg
      481 ccgaagacag cttcgatgat gaactgtatg gcgcggtgat caccgtgcca gcctattttg
      541 atgacgcgca acgccaggcc accaaagacg cagcgcaatt ggccgggctc aatgtgctgc
      601 gcctgatcaa tgagccaacg gctgcggcca tcgcctacgg actcgataac gccagtgaag
      661 gtgtctatgc catttacgac ctgggtggcg gcacctttga catttccatt ttgcgcctga
      721 cccagggcgt gttcgaggtg gtagccactg gcggcgactc agccctgggt ggcgacgatt
      781 acgaccatgc gctggccgac tgggtgctgg cgcaacttgg catgggccgc atggaagatg
      841 ccagcgcggt tgacaagtct gccctgaagg ccgcagcgcg ggcctgtaaa gaagctttgt
      901 cagctactga ttcagtagct ttcagcgcag tattgacggg ggctacagtg tcttttgacg
      961 ttgaaaaggc agattttgag gcggccagcc aggccttgac tgcgcgcacc ctgacggcgg
     1021 tgcgcaagac cttgcgcgat gccgggcttg cgagcgacgc ggtcaagggc gtggtgctgg
     1081 tgggtggcgc tacccgcatg ccgcagatcc ggcaagcggt ggcccggttt tttggccaac
     1141 tgccgctgac caacctgaac cccgatgagg tggtggcttt aggcgcggca attcaggcca
     1201 accagttggc tggcaacaat cccggcggcg atttgctgct gctcgatgtg attccgctgt
     1261 cgctcggggt ggaaaccatg ggcggcctgg tcgagcgcat tgtgctgcgc aacgaaacca
     1321 ttccgactgc caaggcgcag gatttcacca cctacattga cggccagacc gcgctggcgc
     1381 tgcacgtggt gcaaggcgag cgtgatctgg tggccgactg ccgcagtctg gcgcgttttg
     1441 agctgcgcgg cataccgccg atggttgctg gtgcagcgcg catccgcgtc acattcaccg
     1501 ttgatgccga cggcctgttg agtgtgtcgg cactggagca gggcagcggg gtcgaggcgc
     1561 acattgcggt caagccttcc tatggcctgg ccgatgacca gatcgcccgc atgctggccg
     1621 aaagcttcag caccgccgag gccgacatgc tggcgcgcgc cgtgaccgaa gcacgggtcg
     1681 atgccgaccg catggtgctg gccacccaaa gcgccctggc tgccgacggc gatttgctcg
     1741 acgaacagca gcgtgaagaa gtcgaccagt tggtgcgcga gttgcaggca gccagaagcc
     1801 tgagcgatgc cgccgcaatc gaggcggtga ccaaggcgct ggcccagggc accgaagcgt
     1861 ttgccgcgct gcgcatgaac cgcggcattc aaaaggcact ttctggcaag aatattgcgt
     1921 cgatttgaat gactatccgc gccagcgccc ctgctgccag gatcaccggc cccaaccgaa
     1981 cttaagcgag agcgacatgc cgaccatcaa aattttgccc cacgccgaat actgcccgca
     2041 aggcgctgaa gtctcggccc cggccggcac ctcgatttgt gaagcgctgc tggagcacca
     2101 catcaacatt gaacacgcct gcgaaatgag ctgcgcctgc accacctgcc acgtgatcgt
     2161 gcgcgagggc tttgacacgc tggctgcgtc agaggaagaa gaagacgacc tgctggaccg
     2221 cgcctggggc ctacagccgc aatcacgcct gagctgccag gccattctgg cgcagcacaa
     2281 tctggtcatc gaaattccca agtactcgat caaccacgcc aaggaaaacc attgaaatgg
     2341 tgcagggcag gaatttagct ctgcccccaa ccgattgaat gcagcgccat gcgacaaatt
     2401 tttctcgaca ccgaaaccac cggcctgtcg gccgacaacg gcgaccgggt gattgaaatc
     2461 ggctgtgtcg agctggtcaa ccgcaagctg accggcaaca accgccactt ttacctcaac
     2521 ccagggcgcg acagccacga agaggcgttg aaggtgcatg gtttgaccag cgaattcctc
     2581 aaggacaagc cacggtttga ggcggttgcg gcagacctgc tcgactatct gaaagatgcc
     2641 gagctggtga ttcacaacgc cccgttcgac ctgggtttcc tgaacatgga gctggctctg
     2701 atcggcctgc caccgctgcg cggccaggtc gccaatgtga tcgacacgct ggtcatggcc
     2761 aaggaactct accccggcaa gcgcaattcg ctcgacgcgc tgtgcgaccg gctggaagtg
     2821 gacaactctg gccgcgcgct gcacggtgcg ttgcttgatg ccgaactgct ggccgatgtg
     2881 ttcatcaacc tcacgcgtgg tcaggacgcg ctgctgatgg acgacagcag cgccaccgac
     2941 agcgcaggcc tcgccactgc tgcgctcgac ctgagcagct tcatcctgac cgtgttgccg
     3001 gccaaccagc aggaactggc cgcgcatgag gatgtgttga agcaaatcga caagtccagc
     3061 ggcggcaaga cggtttggcg caagctggcc gatgcggcgg tttgacacag gcaggcgagt
     3121 cgatcaacgg ctgcttggct ggcattcagg gcaaatttgt tctttcgcca agtcgttgat
     3181 ttaccgtcat acccgtgcag gcgggaatga agaagatgcc ataagaacaa attgaccctg
     3241 ggctggcact gccccgggtt ggccgcaggc ggccatgaac ggctcaaatt gaccccgcag
     3301 ccaaaacccc tgccagcgcc agttcagcgc ttgcgagtga atgccgagtt ggcctctagc
     3361 gcttatagat aaagcgttgc tagctattta atttatagtg attgtatggc cggttgaagc
     3421 cagctagccg taagcgtatt caaccaccag gtacatctgg ctggctggga cgatgaccac
     3481 gcacaggtac tcaaactgac caccgtcggc gagtttcaag gcggctgggc ggttggcgac
     3541 ttgccaaacc tcgactggct tggcgggaac agatgcgagg cagcgctgcc ggtgcgacgc
     3601 ttcggtcacg ggttgcaaac cgaatgccgc gacaagtgcc acaacggttg ccgggtggtg
     3661 cgcagccaag atgtagccag accggcccga tatcggtgcc caactgtcga tttcctgcag
     3721 cttgatgcca cgcgtctgta ggtgctcgcg caaatccccc ggcgcgccgt tgttgacgga
     3781 acacgctgcc agcatcgccg tggccatgct caatgccagt gcgattcgag tcacgaaagg
     3841 ccgcaagcgc agggccttcg gttgctgatg aagaccggcg ttggcccggc gcgggccgcg
     3901 gccgctaggc ctgtcgggca ctttgaccgt catttcctgg ccttgcgctg cttgtcaaac
     3961 tttcgccagt atgcaaactc atcctcatga acctcaaaat cgacctctgc cacgcgcgcc
     4021 tgaagtcgtg cctgaacatc ggcgacagcc cggttgtaga tgcttggtcc aatgtcttcg
     4081 aggaagtagg ccagcagccc gctggcggcg atgttgccga ttttttcatc gaaatgctcg
     4141 cgaaagtaac gttcaatcga agcaacggct tgttcgcggt ccggttttga aagttcaatg
     4201 cccatgggtt cgctcttgtg atggtcggcg caagtatccc tcacaaggca tggcgcaact
     4261 gcgccagtgg tgttgcagca ggccaatggc gacggggctg tgccaacttt cgcagcggct
     4321 gcaattggtt acagtgccgc atgcatgacc gacccttttc ctctttggca attgccgaca
     4381 ccaggctttc ggtgctggac gactgcaccc cgatggcacc accggacccc ggcgcggcaa
     4441 cgcttttcat gcaaagcagt gtggtgctgc aggccggtca gcttgaagca gccgaggcct
     4501 tgctgcgcga tgccttgcaa ctggccccgc atctggccga agctcacgcc aatctggcct
     4561 ggctgctgga gcgtcggggt ttgccagaag atgccttgca gcactacgca atggccagtg
     4621 aattgcagcc cggcaacgcc cggattcagg tgaattttgg tgcctttctg gcaggccgcc
     4681 agtttcacaa tgcagcagaa gttgcgtatc ggctggcact gctggccgac cctgccatgc
     4741 cgggtgcctg gtgcaacctg ggtgcgctgc aggcgcagac atttcgcgac caggatgccg
     4801 agcagagcct gcgcaccgcc ctgcaacttg atgccaggca tgttgctgcc catgtcaacc
     4861 tggccttcct gctgctgcgc ctggggcggt ttcacgaagg ctggcagcat ctggaatggc
     4921 gcgactggta ccgggccatt gaaacccgcc tggactgccc gcgctggcag ggtcagccgc
     4981 ttgccggttg cccggtgctg ctggtctatg aggccggtca tggcgacatg atccagtttt
     5041 gccgctacct gcccctggtg cgcagccggg gagccagccg ggtcgatgtg ctgtgtcatc
     5101 cggccctgaa agtgttgctg caaagcctgg acggtgtcgg tgaagttatc ggtcttgacc
     5161 aagaggcctc aggccgcacc tggcactatt gggtgccgct gctcagcttg ccttgccatt
     5221 ttggaacacg gcttgacacc attgtggcga acatcccgta cctgcaggca ccggccgacc
     5281 ggttcgacca ttggcgcgcc gagcttttgc gcgtttgccc tgggccggtg ctgcgggttg
     5341 ggctggtttg gcagggcaat gctgattttg aaaacgatgc gcatcgatca ttgcccgacc
     5401 tgggcatcct ggcaccgctt tgggcggttg tcggcgtgca gtttttcagc ttgcaaaagg
     5461 ggcctggcca gcgcgatgtg ttgcgccacc aagccagcca gccgctgctt gacctggctg
     5521 atggtctgca ggattttgcc gacacggcgg ctgtcatcgg gcaactcgac ctggtgatca
     5581 gtgtcgatac ggcagtggcg cacctggctg gcgcccttgg caaaccctgc tgggtgttgt
     5641 tgccagccgt catgaccgac tggcgctggc tggaacagcg caccgattca ccctggtacc
     5701 cagaggtgct gcgcctgttt cgccagccac gccaggtcgg ctgggcaccg gtcattgaac
     5761 aactgcgggt tgccctgacc agcgaagtca acgcataacg gcgaatttgt ggtgcgctta
     5821 ggatgtggtc gggaataact ccccgttttg caccaaggag ttgggatgca
//
LOCUS       DAOSLL010000264         5869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12281_length_5869_cov_4.631407, whole genome shotgun sequence.
ACCESSION   DAOSLL010000264 DAOSLL010000000
VERSION     DAOSLL010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12281_length_5869_cov_4.631407"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1080)
                     /locus_tag="PLB25_12010"
     CDS             complement(<1..1080)
                     /locus_tag="PLB25_12010"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HOE42337.1"
                     /translation="MNDKKSMTFNDLEQWLNERRVTEVECLVPDLTGVARGKILPRGK
                     FTEDRGMRLPQSVVAMGVTGEFPDSGPYYDVIDPTDKDMQLRPDPSTVRIVPWASDPT
                     AQVIHDCFDNQGNLVPFAPRTVLRRVCELFAAEGLQPIVAPELEFYLTARNTDPNTLL
                     RPPIGRSGRAETSRQAYSIDAVNEFDPLFEEIYDYSDKMELNVDTLIHEIGAGQMEIN
                     FFHAEPLGLADEVFFFKRTVRESALRHDMYATFMAKPIAGEPGSAMHVHQSILSIATG
                     KNIFSNEDGSHSDAFMHYIGGLQHYIPAAMVLVAPYVNSYRRLARHTAAPINIEWGYD
                     NRTVGIRSPISSAQARRVENRVIGAD"
     gene            complement(1473..2126)
                     /gene="pdxH"
                     /locus_tag="PLB25_12015"
     CDS             complement(1473..2126)
                     /gene="pdxH"
                     /locus_tag="PLB25_12015"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041790548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HOE42338.1"
                     /translation="MTSIADLRKSYERAELNEDASHVDPLHQFGKWLNEAIASEVPEP
                     NAMTLATVGSNLRPSTRVVLIKGFDAKGIVWFTNYESRKGQELAGNPFAALQFHWVEL
                     ERVVRVEGLVEKVSDEESDAYFRTRPLDSRIGAWASPQSQVISGRGVLIANAARYGAQ
                     FLLQPPRPPHWGGYRLKPDHWEFWQGRKSRLHDRLRYTLRASPTEPGGWWIRERLAP"
     gene            complement(2272..3486)
                     /locus_tag="PLB25_12020"
     CDS             complement(2272..3486)
                     /locus_tag="PLB25_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HOE42339.1"
                     /translation="MGKLLVAGGGIGGLVAALACARVGAQVELFERAAEFAEVGAGIQ
                     LGPNAMRVLYGLGLKDALQPNLAFPERLVVRSAPTGSVLGHLPLGEVAQQCYGAPYAT
                     IHRADLHALLVQTARQRSDVQLNLNSAVLGVVQTEAAVSVQLADEGQVCGDVLVAADG
                     GFSKLRQQLLADGVPQPTGHLAYRAMVQQSDLPAILRSQQVTVWLGPRLHLVQYPVRA
                     GTWLNVVGIVHGQSQGDVSNWDHSANGQDLQRAFAATCEPLIGLIRAIGHWRLWPLSI
                     RPPMSGAHQHAKGRVALLGDAAHPMLPYLAQGAAMAIEDGATLAQVLAAGGIFGPGSG
                     QQRPPDAVPALLQDYARQRWQRNARVQARAIRNGEIFHATGLLRFGRDMAMRLAGERL
                     LDQPWLYGSSPA"
     gene            complement(3665..4060)
                     /locus_tag="PLB25_12025"
     CDS             complement(3665..4060)
                     /locus_tag="PLB25_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42340.1"
                     /translation="MVIIPSVFRGLALAGVGTLLWSAAAMAADTASLADARARFRQEM
                     AVCDSGNSPQDLATCRLEARNALAEASRGGLNDDPGQYSQNALRRCEVQKNEDRADCE
                     MRMTGQGATEGSVAGGGVLRSSVTVVPAR"
     gene            complement(4239..4829)
                     /locus_tag="PLB25_12030"
     CDS             complement(4239..4829)
                     /locus_tag="PLB25_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HOE42341.1"
                     /translation="MKKLFVLALMMLSAAAQAQSAPAQSSQAKKDLVARVLVLQQSSI
                     ELTGQALVERPAQQMLQQAGLALQTRVPAEKREAVAKLVQDDVKKYLDETAPLVRQQA
                     VKLAPTTIGPLLEEKFNEDELKQLIAFMESPVNRKYLQMGNELQKALGEKLVAQTQAS
                     VEPKVKALEQAIVKHLGLPPAPAAKASESNAKAPKK"
     gene            5018..5164
                     /locus_tag="PLB25_12035"
     CDS             5018..5164
                     /locus_tag="PLB25_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42342.1"
                     /translation="MTTPQTAPVCCRGNGTGCSPATLPGALMVHPMPVKKRIDTLSTL
                     ALLT"
     gene            5234..>5869
                     /locus_tag="PLB25_12040"
     CDS             5234..>5869
                     /locus_tag="PLB25_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42343.1"
                     /translation="MNLPPLIQALLSPLRYPAGVTQVELVQTHISWVLLAGDFAYKIK
                     KPVKLPFLDFSSLAQRKHYCDEELRLNRRFAPTIYLDVVGIFNTPQDPCWAGSGEPID
                     YAVKMRRFDETGRLDRVCARGELLPRQVSDLVATLVAFHAAAAVAPAQSRFGSASSII
                     EPARDNFDHLMRLLPGAAIQARLQALRAWTEAQFVQQADAMATRLQAGWVRE"
BASE COUNT         1150 a         1849 c         1728 g         1142 t
ORIGIN      
        1 atcggcaccg atgacacggt tttccacccg gcgcgcctgc gcactggaga tgggcgagcg
       61 aatacccacg gtgcggttgt cgtagcccca ctcgatattg atcggtgccg ccgtgtggcg
      121 ggccagacgg cggtagctgt tgacatacgg tgccaccagc accatggccg ccggaatgta
      181 atgctgcaaa ccaccaatgt agtgcatgaa ggcatccgaa tggctgccgt cctcgttgct
      241 gaaaatgttc ttgccagtgg ctatgctcaa aatgctttga tgcacatgca tggcgctgcc
      301 gggctcgccg gcaatcggct tggccatgaa ggtggcgtac atgtcatggc gcaaggccga
      361 ctcgcgcacc gtgcgcttga agaaaaacac ctcgtcggcc agccccaggg gctcggcgtg
      421 aaaaaagttg atctccatct gcccggcacc gatctcgtga atcagcgtat cgacattgag
      481 ctccatcttg tcggaatagt cgtaaatctc ttcaaacaag gggtcaaact cgttcaccgc
      541 gtcaatgctg taggcctggc gcgaggtctc ggcgcggccg ctgcggccaa ttggcgggcg
      601 cagcagcgtg ttggggtcgg tgttgcgggc ggtcaggtaa aactccagct cgggggcgac
      661 gatcggctgc aaaccctcgg ctgcaaacaa ctcgcaaacc cggcgcagca cggtgcgtgg
      721 tgcaaacggc accaggttgc cctggttgtc aaagcagtca tgaatcacct gcgcggtcgg
      781 gtctgatgcc cacggcacaa tgcgcacggt ggacgggtcc gggcgcaatt gcatgtcctt
      841 gtcggtcggg tcaatcacgt cgtaatacgg tccgctgtcg gggaactcac cggtcacgcc
      901 catcgccacc accgattgcg gcaggcgcat gccacggtct tcggtgaact tgccacgcgg
      961 caaaatcttg ccacgggcga caccggtcaa gtctggcacc aggcactcca cttccgtcac
     1021 gcgccgctcg ttgagccact gctcaagatc gttgaaggtc attgattttt tgtcgttcat
     1081 tggttaatcc tggtgacgtt gaaccgggcc gcatcatttg cccagacgct cattgtgagg
     1141 gctggactga ttcacaacca gtgccagttg atttgagtac atgatgccac tttagctgtc
     1201 atgcacaatt caagccaatg ccactgtact ctgccagacc gcgctggccg aactcttgca
     1261 cagtgagcta tcccgactga agcaggcatc ttggctagcg ctgctgcggc agtggtagct
     1321 ggcactgcgc ggcgcgatgc tgcccggcct tcggtggccg gtgaacgacg gccatcatgc
     1381 caagacctga tgcagcacca gccactggcg cgcagcacgc tgacagtggc tctctggcag
     1441 ttgaccgtga cgtgcatccg gtcgaacgat tgtcacggtg ccagtcgttc cctgatccac
     1501 cacccgccgg gctcagtggg cgaggcccgc agggtatagc gcaggcggtc atgcaggcga
     1561 cttttgcggc cctgccagaa ctcccagtgg tcgggtttga gccgataccc gccccagtgc
     1621 ggcgggcgtg gcggctgcag caagaactgg gcaccgtagc gggccgcatt ggcgatcaat
     1681 accccccggc ccgatatcac ctggctttgt ggactggccc aggcaccaat gcgcgagtcc
     1741 agcggccggg tacgaaagta agcatcgctt tcctcgtcgc tgactttttc aaccaagccc
     1801 tcaacgcgca ccacccgttc cagctcgacc cagtgaaact gcagcgccgc aaacgggttg
     1861 cccgccagtt cctggccctt tcggctctca taattggtga accagacaat gccctttgca
     1921 tcaaacccct tgatcagaac gacccgcgtg ctgggtcgca aattgctgcc cacagtggcc
     1981 agcgtcatgg cattgggttc gggcacttcg gacgcgatgg cttcattgag ccactttcca
     2041 aattgatgca gcgggtcaac gtgcgaggca tcttcattga gttcagcgcg ctcatagctt
     2101 ttgcgcagat cggcgataga ggtcatcagc ccagtatatg ggctcgaact ctggatggcc
     2161 agaaattggg aaaaagccgg gttttgactg ggcaaaccag gtaccctgat caacccagcc
     2221 tggtctggca ccatcggtgc ccgcagccac gaccatcagg ccttggcagg ttcaggccgg
     2281 actgctaccg tacaaccagg gctgatccag caagcgttcg ccagccaggc gcatcgccat
     2341 gtcgcggcca aaacgcagca atccggtggc atgaaaaatc tcgccgttgc ggatggcacg
     2401 cgcctgcacc cgtgcattgc gctgccagcg ctggcgggca tagtcctgca gcaaagcggg
     2461 cacagcgtcg ggggggcgct gttgtcctga ccccggccca aaaatcccgc ccgccgccag
     2521 cacctgggcc agcgtggcac catcttcaat cgccatggcc gcaccctgtg ccagataggg
     2581 cagcatcggg tgtgctgcat cacccagcaa agccaccctg cccttggcat gctggtgggc
     2641 accgctcatg ggcgggcgaa tgctcagggg ccacaggcgc cagtggccaa ttgcgcggat
     2701 caggccgatc agcggctcgc aggttgcggc aaaagcccgc tgcagatctt gcccgttggc
     2761 actgtgatcc cagttcgaca cgtcaccctg ggactgtcca tgcacgatgc ccaccacatt
     2821 gagccaagtg ccggcacgca ccgggtactg caccaggtgc agcctcgggc ctagccagac
     2881 ggttacctgc tggcttcgaa ggattgccgg caaatcactt tgctgcacca tcgcacggta
     2941 ggccagatgc ccggttggct ggggtacgcc gtctgccagc aattgctggc gcaacttgct
     3001 aaagccccca tcggcggcca ccagtacgtc gccgcaaacc tgaccttcgt ctgccaattg
     3061 cacgctgact gcggcttcgg tctgcaccac acccagcacg gcgctgttca aattgagttg
     3121 cacatcactg cgttgacggg ccgtttgcac cagcaaagca tgcaaatctg cgcggtgaat
     3181 ggtggcataa ggcgcgccat aacactgttg ggccacttcg cccaggggca ggtgccccaa
     3241 aaccgagcca gtcggggcac tgcgcaccac caaacgctcg ggaaacgcca agttcggctg
     3301 cagcgcgtct ttcaggccca agccatacag cacccgcatg gcgttggggc ccagttggat
     3361 gccggcaccc acctcggcaa actcggcagc gcgctcgaac aattcgacct gggcgccgac
     3421 acgggcacag gccaaggcag ccaccaggcc accaatgccc ccacccgcaa ccaacaactt
     3481 gcccatagcg cctctttctc tgaccccatc gttcaggcgt attgtggaca agcatcaatt
     3541 cagtaagctg acaatgcttg catcaggtcg ggcagccgca cggctaaatg tccggccggg
     3601 catcgccgcc actgcggatg gcggtgctgg tgatcgtggt gatagatgtc ggaagatgtc
     3661 tggttcacct ggccggcaca acggtgacgc ttgagcgcag cacgccgccg cccgccacgc
     3721 taccttcggt ggcaccttga cccgtcatgc gcatttcgca atcagcccgg tcttcattct
     3781 tttgtacttc gcaacggcgc aaggcgtttt gcgaatactg gccggggtca tcgttgaggc
     3841 cacctcgaga ggcttcggca agtgcgttgc gcgcctccaa acggcaggtc gccaggtctt
     3901 gcggcgaatt gccgctatcg caaaccgcca tttcctgccg aaaacgtgcc ctggcatcag
     3961 ccagcgaagc ggtgtctgct gccattgcag cggcagacca gagcagcgtg ccgacgccag
     4021 ccagggcaag accgcgaaac acagaaggga tgatgaccat gtcgaactcc ttttcatgac
     4081 gatgacccaa tgcttcggtc cgaaaatggg tgatggactc cagaggcatc ttcctgcggt
     4141 tgcaggagga cgcactgtcg cagcaaacca cctggcagtc tgtgcgcaat cgcacccacc
     4201 accttggctg ctgtcggttg cccaaaaacg gattccggct attttttagg tgcttttgcg
     4261 ttgctctctg acgctttggc ggcaggtgcc ggcggcagac ccagatgttt cacgatcgcc
     4321 tgctccagcg ccttgacctt gggctcaacc gacgcctggg tctgggctac cagcttttca
     4381 cccagggctt tttgaagttc attgcccatc tgcaaatact tgcggttgac gggagattcc
     4441 atgaaggcga tcagttgctt gagctcgtct tcgttgaatt tctcttccag cagcgggccg
     4501 atggtggtgg gtgccagctt gacggcctgc tgccgcacca gcggagcggt ctcgtcaagg
     4561 tatttcttga catcgtcctg aaccagcttg gccaccgcct cacgcttttc agccgggaca
     4621 cgggtttgca gcgccaaacc cgcctgctgc aacatttgct gcgcggggcg ctcaaccagt
     4681 gcctgcccgg tcagctcgat cgaactctgc tgcaacacca gcacgcgagc caccaggtct
     4741 tttttggcct gggatgattg cgccggggct gattgcgcct gggcagcagc ggacaacatc
     4801 atcaaggcca acacgaacaa ttttttcatt gcattttctc tctggcaaac tgccattagt
     4861 gggtaaaaaa tggaccaccc aggcgcagta agcttgcgca ggcggccccg gtcaaaccca
     4921 cgagcttaac ccaagccgga ccagcaggtt gcaaaccagc aacaccaggg tgccagatgg
     4981 tgatgacgga gcagcaattc gggaacacaa gtgatcagtg accaccccgc agaccgcccc
     5041 ggtgtgctgc cgtgggaacg gcaccggttg ctcccccgcc accttgcctg gggctttgat
     5101 ggtgcatccg atgccggtaa aaaagcgcat tgacacgctg agcaccctgg cattgttgac
     5161 ctgatggccc aatcgtcaca ccctttgcgc tttgtcatca cggccgccgg cgcaatctgg
     5221 caacatggcg tgcatgaact tgccgccgct gattcaagcc ctgctgtcgc ccttgcgcta
     5281 tccggccggg gtgacacagg ttgaactggt gcagacccat atctcatggg tgctgctggc
     5341 aggcgacttt gcctacaaaa tcaaaaaacc ggtcaagctg ccatttctgg acttcagttc
     5401 gctggcgcag cgcaagcatt actgcgacga ggaattgcgc ctgaatcgcc gctttgcgcc
     5461 gaccatctac ctcgacgtgg tcggcatttt caacacaccg caagacccgt gctgggcagg
     5521 cagcggcgag ccaatcgact atgccgtcaa aatgcgccgc tttgacgaaa ctggccgact
     5581 tgaccgggtc tgcgccaggg gtgagctgtt accccggcag gtgtcggatt tggtagcgac
     5641 gctggtcgcg tttcacgctg ctgcggcagt cgcgccagcg caatcgcggt ttggctcagc
     5701 cagcagcatc attgagccgg cgcgggacaa ttttgaccac ttgatgcgac tgctgcccgg
     5761 ggcagcgata caagcccggc tgcaagcgct acgggcctgg accgaggcgc agtttgtcca
     5821 acaggccgac gcgatggcga cacgcctgca ggccggttgg gtgcgcgaa
//
LOCUS       DAOSLL010000265         5866 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12293_length_5866_cov_4.120633, whole genome shotgun sequence.
ACCESSION   DAOSLL010000265 DAOSLL010000000
VERSION     DAOSLL010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5866
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12293_length_5866_cov_4.120633"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..701)
                     /locus_tag="PLB25_12045"
     CDS             complement(24..701)
                     /locus_tag="PLB25_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HOE42344.1"
                     /translation="MQDSSLMQLMWLASPALPVGGFSYSECLEAAVDSALVATENEAS
                     EWLLEQLHTSLARSDLAVIAQAVSAWQAHDVARIAQLNAWVLMTRESSELRAQTEQMG
                     RSLLEWLRNHTTAGPQHIKLLADLQPTYPLAFALAASATGAALRDCLLSYAFGWAENM
                     VQAAIKAVPLGQSAGQRILSALVAAIPAAVDLALSLDDDSRQAFSPMLAILSARHETQ
                     YSRLFRS"
     gene            complement(999..1535)
                     /gene="ureE"
                     /locus_tag="PLB25_12050"
     CDS             complement(999..1535)
                     /gene="ureE"
                     /locus_tag="PLB25_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HOE42345.1"
                     /translation="MLQTSKLIAQGKGLAAALLKRASTVELDWDVRQKSRFEAIDSTG
                     RQIGVFLPRGAVARGGDVLVAQDGSLIKVIAAPQAVLKITHCPNHGSAFDLIRAAYHL
                     GNRHVPIELKPDHLKIEPDHVLAAMLGAMHLMVTEVCESFEPENGAYATGGHFGGHRH
                     SHDDEPAHAHGPGCGHSH"
     gene            1861..2187
                     /locus_tag="PLB25_12055"
     CDS             1861..2187
                     /locus_tag="PLB25_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42346.1"
                     /translation="MKRELNCTDFMISRAATPLPGELRAGYPVITIIGPRQSGKTTWA
                     RAAFPDKPHVSLETPVGREFAATDPRNLLPRCSGGATILGACRTYGTSAANRPQRSSL
                     HRLFDP"
     gene            3395..3805
                     /locus_tag="PLB25_12060"
     CDS             3395..3805
                     /locus_tag="PLB25_12060"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE42347.1"
                     /translation="MKKVNYTNNRRILVVDDVEVHRVMMDSFLRKINPFLTVEEASTV
                     PEAIEKLGNGKFDAVVSDWNLPDHGGDVLLKWMRSRANFNRVPFIMVSSNTDNEDIIH
                     AFMKLGVDAYVTKPFKAQDLYEKIVAAYDKRNPG"
     gene            complement(3923..5686)
                     /gene="ureC"
                     /locus_tag="PLB25_12065"
     CDS             complement(3923..5686)
                     /gene="ureC"
                     /locus_tag="PLB25_12065"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HOE42348.1"
                     /translation="MATIGRHAYAEIFGPTVGDRVRLADTGLLIEVEKDYTLAAGGYG
                     EEVKFGGGKTIRDGMAQSQRTRFEGAVDTVMTNALILDHWGIVKADIGLKDGRIVGIG
                     KAGNPDVQPGVDIIIGPGTEVISCEGNIVTAGGIDSHIHFICPQQIEEALASGVTTML
                     GGGTGPATGTFATTCTPGPWNIERMLQAADAFPMNLGFLGKGNASLPGALHEQVNAGV
                     IGLKLHEDWGTTPAAISNCLDVADETDVQVAIHSDTLNESGFVENTIAATKGRGLCAF
                     HTEGAGGGHAPDILRVVGEANFLPSSTNPTMPFTVNTLDEHVDMLMVCHHLDAGIAED
                     LAFAESRIRRETIAAEDVLHDLGAISMMSSDSQAMGRVGEVIIRTWQTAHKMKAQRGW
                     LAPPLVLGEDRGAGVLAEYDQRNDNFRARRYVAKYTINPAIAHGISHEVGSIEIGKWA
                     DLVVWKPAFFGVKPALILKGGFISMAAMGDPNASIPTPQPVHYRPMFGAFGGALARGS
                     LTFVSQAGLNAGIKNRFGLAKQLSAVKNIRGVRKQHMVHNHYLPNMEIDAQTYSVRAD
                     GQLLTCEAAVTLPMTQRYFLF"
BASE COUNT         1180 a         1688 c         1699 g         1299 t
ORIGIN      
        1 acctggcgct tgctgtataa gggctaggac ctgaaaagcc ttgaatactg ggtttcatgc
       61 cgggccgaca gaatggccag catggggcta aaggcctgac ggctgtcgtc atccagactg
      121 agcgccaggt ccaccgccgc aggaattgct gccaccaggg ccgacaaaat acgctgcccg
      181 gcgctttgac ccagtggcac tgccttgatg gccgcctgca ccatgttttc ggcccagcca
      241 aaggcgtagc tcagcaggca gtcacgcagc gctgccccgg tggcactggc cgccagtgca
      301 aaggccagcg ggtaggtcgg ctgcaaatcg gccagcagct tgatgtgttg cggcccggcg
      361 gtggtgtggt tgcgcagcca ctccagcaag gatcggccca tttgttcggt ctgcgcgcgc
      421 agttcgctgc tctcgcgggt catcagcacc caggcattga gctgcgcgat gcgtgccaca
      481 tcgtgtgcct gccaggctga aacagcctgc gcaatcaccg ccaggtcaga ccgcgccagg
      541 ctggtgtgaa gctgctccag cagccattca gacgcttcat tttcagtagc taccagggca
      601 ctatcgacgg cggcttcaag gcattctgag tatgaaaatc cacccaccgg caaggccggt
      661 gaggcgagcc acatcagttg catcaggctg ctgtcttgca tgcttgggcg gccctgtggg
      721 cgtgtcgttg gcaagtgtgt cgggtcgcca cctggcgttg cctgacgggc cgttgctggc
      781 cctgaccgag ggcagcgaaa acgacgcgtt gatgttgtga acggcgtagc agaattgggg
      841 tcggatccca attcggcccg gcgcattcaa tcggatcaac aggtttcgtc cgaattgggc
      901 tctgacccca tttctgcagg gcagactcac ggcgcggcgg ttggcagcac attcggcaaa
      961 gtttcatgct gtcaggtaga tcagcatcga ttgccaaatc agtggctgtg accacaacct
     1021 ggtccatgcg catgcgccgg ttcatcgtca tggctgtgcc gatgcccgcc aaaatggccg
     1081 ccagtggcgt aagccccgtt ttccggctca aacgactcgc agacctcggt caccatcaag
     1141 tgcatggcac ccagcatggc cgccagcaca tggtctggct cgattttcag gtggtctggc
     1201 ttgagctcaa tcgggacatg ccggttgccc agatggtagg cggcgcggat caagtcgaat
     1261 gcgctgccat gattggggca atgggtgatc ttcagcaccg cttgcggtgc tgcaatcacc
     1321 ttgatcaatg agccatcctg ggccaccagc acgtcgccac cgcgcgccac cgcgccgcgc
     1381 ggcagaaaga ccccgatctg ccgtccagtt gaatcgattg cttcaaaacg gcttttctgg
     1441 cgcacatccc agtccagttc aacggtgctg gcccgcttga gcagcgcagc cgccaggcct
     1501 ttgccttggg cgatgagttt ggaggtttga agcatggtgt tgatactgtt caaaatacgg
     1561 cgttgtggac accgcaaaag ccgtgccggg ccccaagcgt cggttgacag atgccggtat
     1621 tgacgatggc tatggctgtt tgcaactgat gaagcttgag gtgggttcga actggggcag
     1681 aaccagttta ccgcctccat cggcagtctt tcccggtcac ctcggattgg ctcgcgcttg
     1741 ggggttcgac gcacagctct cttggcccgg cgcaacaatg cagttttgaa tcaggccatt
     1801 ggcgaccact gtgcctcaag gttttggcaa tttgatcggt ttcaatcgtc ttgcattgcc
     1861 atgaaacgcg agctcaattg cacggatttc atgatttcac gcgctgccac cccgttgccc
     1921 ggcgaactgc gcgcaggcta tccagtcatt accatcatcg ggccacgcca gtctggcaaa
     1981 accacctggg cgcgggcggc ttttcctgac aagccccatg tgtcgctgga aacgcccgtt
     2041 gggcgtgaat tcgcggccac tgacccgcgc aatttgctgc cgcgctgctc cggcggtgcc
     2101 actatcctag gagcctgtcg gacttatgga acatcggctg caaatcgacc gcagcggtcc
     2161 agtttgcacc ggctttttga cccatagcgg ggctatgggc ctgcaaatcc gacgcaaact
     2221 ggcctcgctg gggccgattc tcgcgataga tgccatatgt ccgacaggct cctggatttg
     2281 gttgaccact tgctttcatt ttcaagtcat catgagcagt tactctttta gcttcggtgg
     2341 ggttcacctt gtgggtggca gcgccaggca cccgattgag ccgctggcaa cgcgactggg
     2401 ggcgttgcgc ctgcctcgtc cgcaaggacg gacatgctct cagtagctga gcgtcgtcgt
     2461 cacccctgca gggcgcagcc atgtcagagg catggcctgc ttcaagccgt ccaaacgcgc
     2521 gcaggcacgt ttggagtcgc ggctttcgcc actacccagt cgcgccgcca agggcccact
     2581 cgggcgcgtt caactgagga atccatgttg aacatgcatt tcctccaagg cccgtcttct
     2641 gccaaagtca atttccagcg cgtcttgcag cgaatgcagt attgaatatc aggcctaaga
     2701 gaagcactgc gtcgctataa ttcacttgcc ctttgcagtg agttggaacg caagctactc
     2761 gccgccagtc tcaatagcat taattgattg attgcaataa tgtctgacct ctctgccagt
     2821 accgagcaac caccgatcaa ttttgaccac gcactcaaag cggctttcgg caatgccaag
     2881 cttcttacaa aggttgtggg attgttgctc aaacaaatgg acgttgacct acccgccatt
     2941 cgcgctggtg tggcagccag agactcaccc gcacttgcca gctcatctca tcgactcaaa
     3001 ggcagtttgg gtgctgttga ggcaaagcct gcgtacctgt cctgcacggc actcaataca
     3061 tcggcgcgca gcgatgcgct tgaatcgtat gagcctgaat tggcccttct ggaacatgaa
     3121 ctggcccggc tgcgaccgtg tttgcaggct tggctcatcg aacatacata gtgagcatct
     3181 agtgtctgaa cgaaagtcat ttttcttata aaaatcatat tgttagatca cctttctaaa
     3241 gcgaagattt ttgaaaaacg gtcttattgc gcgactttca gaaaatcgct ctggggaaag
     3301 caaaattttg agcaaaatct tcgtttcaaa aaaatgatgc aacttgctga tttagattaa
     3361 caaacagggt tttcgttcag atactgtcta gaccatgaaa aaagtgaatt acaccaacaa
     3421 ccgccggata ttggtcgtcg atgacgtgga agttcaccgc gtcatgatgg attcgtttct
     3481 gcgtaagatc aatccgttcc ttaccgtcga ggaggcctcg acagtgcccg aggccatcga
     3541 gaagctcggt aacgggaagt ttgacgcagt ggtttctgac tggaatctgc cagaccatgg
     3601 tggagatgtc ttgttgaaat ggatgcgctc ccgggcaaac ttcaaccggg tgccattcat
     3661 catggtttct agcaatacag acaatgaaga catcatccac gcattcatga agctgggggt
     3721 agatgcctac gtcaccaagc cgttcaaggc ccaggattta tatgaaaaga ttgttgccgc
     3781 ttacgacaag cgcaatccgg gctgatgcgt cgggcacatt catcggcgtg gtttgatttc
     3841 gttttgccaa atttgattgg cttgatgatc acgaaacggc agttggcagg catggcagac
     3901 aatcgctggc agccaatttg gctcaaaaca aaaagtacct ctgcgtcatc ggcaacgtca
     3961 cggcggcctc acacgtcaac aactggccat cagcccgcac gctgtaggtt tgggcatcga
     4021 tttccatatt gggcaaatag tggttatgca ccatgtgttg tttgcgaacg ccacggatgt
     4081 tcttcactgc gctgagttgt ttggccaggc caaagcggtt cttgataccg gcatttaacc
     4141 cggcttgcga gacaaacgtc agcgaaccac gcgccaaagc cccgccaaaa gcaccaaaca
     4201 taggccggta atgcaccggc tgtggcgtcg gaatcgacgc attggggtca cccatggcag
     4261 ccatggagat gaagccaccc ttgagaatca gcgctggctt cacgccaaaa aacgctggct
     4321 tccagaccac cagatcggcc catttgccaa tctcgatgct gccgacctcg tggctgatgc
     4381 cgtgcgctat tgccgggttg atggtgtact tggcgacata gcgcctggcg cgaaagttgt
     4441 cattgcgctg gtcgtattcc gccagcaccc cggcaccccg gtcttcgccg agcaccagcg
     4501 gcggtgccag ccagccccgc tgcgccttca tcttgtgcgc ggtttgccag gtgcggatga
     4561 tgacttcgcc aacccggccc atggcctggc tgtcgctgct catcatggaa attgctccca
     4621 ggtcgtgcag cacgtcttca gcggcgatgg tttcgcgtct gatgcggctc tcggcaaagg
     4681 ccaggtcttc ggcaatgcca gcatccagat ggtggcagac catcagcatg tccacatgct
     4741 cgtcgagcgt gttgacggta aacggcatcg tcgggttggt cgaactgggc aaaaagttgg
     4801 cttcgccgac cacccgcaga atgtccggcg catggccacc gccagcgcct tcggtgtgaa
     4861 aagcgcacag gccgcggcct ttggtggcgg caatggtgtt ttcaacaaag cctgattcgt
     4921 tgagtgtgtc agagtggatg gcgacctgga catcggtctc atcggccacg tccaggcagt
     4981 tgctgatggc cgctggtgtg gtgccccagt cttcgtgtaa tttgaggcca atcacgccag
     5041 cgttgacctg ctcgtgcaag gcaccgggca ggctggcatt gcccttgccc agaaaaccca
     5101 ggttcatggg gaatgcatcg gcggcctgca acatacgttc gatgttccac ggccccggtg
     5161 tgcaggtggt ggcaaaggtg ccggtggccg ggccggtgcc gccgccgagc atggtggtga
     5221 caccgctggc cagcgcttct tcgatctgct gcgggcaaat gaaatggatg tggctgtcga
     5281 tgccgcccgc cgtgacgatg ttgccctcgc aactgatgac ttcggtgccg gggccgatga
     5341 tgatgtccac ccccggctgt acgtcgggat tgccggcctt accgataccg acgatccgcc
     5401 cgtctttcag gccgatgtcg gctttgacaa tgccccagtg gtcgaggatc agggcattgg
     5461 tcatcaccgt gtccacggca ccctcaaagc gggtgcgctg tgactgcgcc atgccgtccc
     5521 gaatggtttt accgccgcca aacttgacct cttcaccata accgcctgcc gccagggtgt
     5581 agtctttttc gacttcaatc aacaagccgg tgtcggccag gcgcacccgg tcgcccacgg
     5641 tgggaccaaa aatttcagcg taagcgtgtc gtccgatggt tgccatgtca agctttcatg
     5701 cgtgggtggg ttgcggctgg atgcaaggtg ggcagggtcc gtggaacagg gttctgatgc
     5761 caaagctgct gcggttgcag cgttggcgct ttgtcgcagc ggtgctgatg gggtgctcga
     5821 tcacctcaaa ccttggacag gctgccctgc gtcaggccac gaaaac
//
LOCUS       DAOSLL010000266         5841 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12366_length_5841_cov_4.563775, whole genome shotgun sequence.
ACCESSION   DAOSLL010000266 DAOSLL010000000
VERSION     DAOSLL010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5841)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5841
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12366_length_5841_cov_4.563775"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="PLB25_12070"
     CDS             complement(<1..238)
                     /locus_tag="PLB25_12070"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073020.1"
                     /note="catalyzes the formation of tyrosyl-tRNA(Tyr) from
                     tyrosine and tRNA(Tyr); Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HOE42349.1"
                     /translation="MNQAKTPPFPVTDRVLEALAVTLRGCEELIPQDDWVKKLARSAA
                     TGTPLRIKLGLDPTAPDIHVGHTVVLNKMRQLQDL"
     gene            378..1739
                     /locus_tag="PLB25_12075"
     CDS             378..1739
                     /locus_tag="PLB25_12075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOE42350.1"
                     /translation="MINSFVNRGHALLEGLAHSSARHPKRVTSAIAALMLGGGGAAFA
                     LASLAPDASDLPVRQVIESVQPAGSASMAVQPVLNLYRTDITRSSDTADTLLKRLGVD
                     DAAAARFMRGDALARQMLGRSGRNVTAETDQDQSLLKLSARWSPDDSGQFKRLVVQKT
                     PKGFASRLESAPLVATTRLASGSIRTSLFAATDDAGLPDAIATQLAEVFSSDIDFRRA
                     LRKDDRFNVVYEVLEGDGEPMRTGRVLSAEFVNAGKTFQAMWFQPPGQDGQGAALKGG
                     YYSLDGTSLRRAFLSSPVEFSRVSSGFAMRFHPILQQWRAHLGTDFAASTGTPARTVG
                     DGVVEFAGGQSGYGNVVFIKHRNNTETVYAHLSKIMVQRGQSVSQGQTIGLVGATGWA
                     TGPHLHFEVRVGGVQHDPMTIAQKSETVPVSAAMRPLFKQAAASVKSELQAAASINPT
                     RFE"
     gene            2008..3147
                     /locus_tag="PLB25_12080"
     CDS             2008..3147
                     /locus_tag="PLB25_12080"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HOE42351.1"
                     /translation="MPELYIGLMSGTSLDGVDGVLADFSEPGVRVLASASRPFSAAFR
                     RELLALNSPSPNELHRAALAGNQIAAVYADVVADLLAQAALGGIEKSGIRAIGAHGQT
                     VRHQPQMATGTGNAAAAYTLQLNNPAMLAERTGIDVVADLRSRDVAAGGQGAPLVPAF
                     HRHVFSSADDSVAVLNIGGIANLSVLPNDAARPVLGFDCGPGNALMDAWCQRHTGQAF
                     DAEGAWAASGQCLPDLLQHLLNEPYFSQDPPKSTGRDLFSSQWLADKLLPFAAALPQD
                     IQNTLTELTASACADCVISYAKYSSLLIVCGGGAFNRYLLARLQALLPTLPVSTSDQH
                     GLPPLQVEATAFAWLARQAMQRLPGNLPSVTGAAGARILGAIYPA"
     gene            complement(3209..3574)
                     /gene="erpA"
                     /locus_tag="PLB25_12085"
     CDS             complement(3209..3574)
                     /gene="erpA"
                     /locus_tag="PLB25_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HOE42352.1"
                     /translation="MSAVAEDIQTEMPAPILFTDSAAAKVADLIAEEGNPDLKLRVFV
                     QGGGCSGFQYGFTFDEVTNDDDTTMTKNGVSLLIDAMSYQYLVGAEIDYKEDLQGAQF
                     VIKNPNATTTCGCGSSFSA"
     gene            complement(3633..4037)
                     /locus_tag="PLB25_12090"
     CDS             complement(3633..4037)
                     /locus_tag="PLB25_12090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HOE42353.1"
                     /translation="MFFKKKQPLIKSLVAHGCQITGNFSFADGFRLDGELSGNIVGQA
                     DKPSILVISESAHVTGKVEADHVIINGQVDGPVHARVMLEMQPKARITGDVTYKLLEM
                     HQGALVDGRLCPIVAASQADEKPTLKLAANNP"
     gene            complement(4284..5024)
                     /locus_tag="PLB25_12095"
     CDS             complement(4284..5024)
                     /locus_tag="PLB25_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42354.1"
                     /translation="MRLRLLLRRLTVSAPRMAVRSALPWPVRWVMLATVAGFCAAVAL
                     WAFEFGREIAGLDHGTKEQLQQAQTQLDALRTQLDAVTVERDKAQSIANTADTVLMTE
                     KVAQEKLAALNKQLALDNQRLKDDLGFFEQLIPAADTGVNAGALAVRGLQAELLKTGE
                     LKWQVLVIQAVKNPAEFNGQLEVSFVGVSAGRPWTGTLADGAVALKVRQYARLAGVYQ
                     VPAPVVVKSMTVKVLDGKTVRTVQTVKL"
     gene            complement(5106..5498)
                     /gene="rpsI"
                     /locus_tag="PLB25_12100"
     CDS             complement(5106..5498)
                     /gene="rpsI"
                     /locus_tag="PLB25_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019447061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HOE42355.1"
                     /translation="MIGEWNNGTGRRKSSVARVFLKKGSGQIVVNGKAIENFFGRATS
                     IMICKQPLLLTNHAETFDVMVNVAGGGESGQAGAVRHGITRALIDYDATLKPDLSKAG
                     FVTRDAREVERKKVGLHGARRRKQFSKR"
     gene            complement(5510..>5841)
                     /gene="rplM"
                     /locus_tag="PLB25_12105"
     CDS             complement(5510..>5841)
                     /gene="rplM"
                     /locus_tag="PLB25_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HOE42356.1"
                     /translation="LRLRGKHKAIYTPHVDTGDFIIVTNAAQLRVTGAKSLDKMYYSH
                     SGYPGGISSTNFRDMQAKFPGRALEKAVKGMLPKGPLGYAMIKKLKVYGGAEHPHTAQ
                     QPKVLVL"
BASE COUNT         1150 a         1770 c         1671 g         1250 t
ORIGIN      
        1 ccaggtcctg caactggcgc atcttgttga gcaccacggt atggccgaca tggatgtctg
       61 gcgcagtcgg gtccagcccc agcttgatcc gcagcggggt gccggtagcg gcagaacggg
      121 ccagtttttt gacccagtca tcctgcggaa tcagttcctc acaaccgcgt agcgtgaccg
      181 ccagtgcctc cagcacacga tcagtgacag gaaaaggcgg tgttttggct tggttcatac
      241 gggtttatct ggatagatcg tgtttggcgg ggtttatact cgatgttcgc ttttcggtct
      301 gttccaaatt gaccatagca actactttgt ttgcttgtgt tgcgtgatta ccacaggctc
      361 aacttcagga ctttcttttg atcaactctt tcgtgaatcg tggccatgcc ctgcttgagg
      421 ggcttgccca ctcaagcgcc cgccacccca aacgtgttac ttctgccatc gctgccctga
      481 tgcttggtgg cggcggcgct gcgtttgcgc tggcatcgct ggcccctgat gcatccgatc
      541 tgccggtgcg ccaggtcatt gaatcggtgc aacctgcggg cagcgccagc atggcggttc
      601 agcctgtgct caacctctac cgcacggaca tcacccgcag cagcgacacc gccgacacgc
      661 tgctcaagcg cctcggtgtc gatgacgcag cggcggcccg cttcatgcgc ggtgacgcac
      721 tggcgcgcca gatgctcggg cgcagtggcc gcaacgtcac cgccgagacc gaccaagacc
      781 agagcctgct caagttgagc gcccgctgga gccctgacga cagcggccag ttcaagcggc
      841 tggtggtgca aaaaacgccc aaaggctttg cctcgcggct cgaatctgcg ccactcgtgg
      901 caaccacacg cctggccagc ggcagcatcc gcacatcctt gtttgctgcc accgatgatg
      961 ccggtttgcc agatgccatc gccacgcaat tggccgaggt tttttccagt gacattgatt
     1021 ttcgacgcgc cctgcgcaaa gacgaccggt tcaatgtggt ctatgaagtg ctggaaggcg
     1081 acggcgaacc gatgcgcacc gggcgtgtgc tcagcgccga gtttgtcaac gctggcaaga
     1141 cttttcaggc aatgtggttc cagccgcccg gccaggacgg ccagggagca gctctgaagg
     1201 ggggctatta ctcgcttgat ggcaccagcc tgcgtcgggc gtttctgagc tcgccggtcg
     1261 agttttcgcg ggtcagcagt ggatttgcca tgcgctttca cccgatcctg cagcagtggc
     1321 gcgcccatct gggcactgac tttgctgcca gcaccggcac acctgcacgc accgtgggtg
     1381 acggcgtggt cgaatttgcc ggcggacaaa gcggctacgg caatgtggtt tttatcaagc
     1441 accgcaacaa taccgaaacg gtgtacgccc acctcagcaa gatcatggtg cagcgtggcc
     1501 aaagcgtgtc acagggccag acaattggcc tggtgggtgc caccgggtgg gccaccgggc
     1561 cgcatctgca ttttgaggta cgcgtgggcg gtgtccagca cgacccgatg acgattgccc
     1621 aaaagagcga gacggtgccg gtctctgccg caatgcggcc gctattcaag caggcggcgg
     1681 ccagcgtcaa gagcgaattg caagccgctg ccagcatcaa tccgacccgg tttgaataga
     1741 accagactcc ccagttgacc gcttgttttc atcggtaagt ccctatgact atttactttt
     1801 taagtttcga tggggttcac ctgttgggtt acagtgcgac gcacccgatt gagccgctcg
     1861 ggcgtgtcca actgagcaat cctggataaa cccatctgtc tttcactgtt ctcctgcatg
     1921 cgccgccacc acagccatag cgtgcaggta gttctggcca ggcctggtct ggcacaccgg
     1981 tggcgctgag aaaccatgca caaccccctg cccgagcttt acattggcct gatgtcaggc
     2041 acctcgctgg acggggtgga tggtgtgctg gcagatttct ctgagcctgg cgtgcgggta
     2101 ctggccagcg catcaaggcc attcagtgct gctttccgcc gcgaactgct cgcgctcaac
     2161 agcccatctc ccaacgagtt gcaccgcgcc gcgctggctg gcaaccagat tgccgcagtc
     2221 tatgccgatg tcgtggctga tttgctggcg caagcggcgc ttggcggcat cgaaaaatca
     2281 ggcattcgcg ccatcggtgc ccatggccag acggtgcgcc accaaccgca aatggcgact
     2341 ggaactggca acgccgctgc tgcctacact ttgcagttga acaatcctgc gatgctggcc
     2401 gagcgcaccg gcatcgatgt ggtggccgac ctgcgcagcc gggatgttgc tgctggcggc
     2461 cagggcgcac cactggtgcc ggcttttcac cgccatgttt tcagcagcgc cgacgacagc
     2521 gttgcggtcc tgaacatcgg cggtatcgcc aacctgagtg tgctgccaaa tgatgcagca
     2581 cggccggtgc tcggctttga ctgcgggccg ggcaacgcct tgatggatgc ctggtgccag
     2641 cggcacaccg ggcaagcttt tgacgcggaa ggtgcctggg ctgccagtgg tcagtgcctg
     2701 cctgacctgc tgcaacatct gctgaacgag ccttacttca gccaagaccc gccgaaaagc
     2761 accggcagag accttttcag ctcgcagtgg ctggccgaca aactcctgcc atttgccgca
     2821 gcgctgcccc aagacattca aaatacattg acggaattga ccgctagcgc ttgtgctgat
     2881 tgcgttatta gctatgctaa atatagcagt ttattgattg tgtgtggtgg cggcgcgttc
     2941 aaccgctact tgctggcgcg cctgcaagcc ctgttgccaa cgctgccagt cagcacttca
     3001 gaccagcacg gcctgccacc actgcaagtc gaggcaaccg ccttcgcctg gctggcgcgc
     3061 caggccatgc agcgcctgcc gggcaatctg cccagtgtga ccggcgctgc tggtgcgcgc
     3121 attctgggtg ccatctaccc ggcttgactc gccagcggca ggcacaaaaa aagccgcccg
     3181 ggcgggcggc ttttcgggca tggaatactc aggctgaaaa actcgaacca caaccacagg
     3241 tggtggttgc gttggggttc ttgatgacaa actgggcacc ttgcagatct tccttgtagt
     3301 cgatctcggc acccaccagg tattggtaac tcatggcatc aatcagcagc gacaccccgt
     3361 ttttggtcat ggtggtgtcg tcatcattgg tgacttcatc aaaggtaaag ccatattgaa
     3421 aacccgaaca gccaccacct tgcacgaaca cgcgcagttt caggtcgggg ttgccttctt
     3481 cggcaatcag gtcagccacc ttggcagcag cactgtcggt gaacaggatc ggggcgggca
     3541 tttcggtctg gatgtcttcg gcaacagcgc tcatggtggt cttccggtaa attgaatggc
     3601 caaatgctac agcaaatttg tcgggcattg cgtcagggat tgtttgccgc cagtttcagt
     3661 gtgggttttt cgtcggcttg cgatgcggcg acgatcgggc aaagcctacc gtccaccaac
     3721 gcgccctgat gcatttccag cagtttgtaa gtgacatcac cagtgattct ggccttgggc
     3781 tgcatttcca gcatgacgcg cgcatgcacc ggcccgtcaa cctgaccatt gatgatgaca
     3841 tggtccgcct cgactttgcc ggttacatgg gccgactctg atatcaccag aatgctcggc
     3901 ttgtcagcct ggccgacaat attgccgctg agttcaccgt cgagtcgaaa tccatcggca
     3961 aaactgaaat tgccggtgat ctggcaacca tgggctacca ggctcttgat caagggttgc
     4021 ttttttttga agaacataaa acaccttttt ctcaaacaat ctcaaggttg gcttgtgcta
     4081 cccggtcgcc agcacacagg cgcaccagcc cggcggaacc tgttagcctg ccagacttat
     4141 gcaacatctg ccgggagtca accgcagcgg ctcattttgc accgggttgt tggcccgcaa
     4201 ctggtcgatg gacctgcaaa tccggcaaaa ctggcctggc tggggtcgat tctcgcagct
     4261 cgatccgtca gtccaaccgg accctagagt ttcacggtct gcacggtcct gaccgtcttg
     4321 ccatcgagca ccttgacggt catgctcttg acaaccaccg gtgcgggcac ctggtaaacc
     4381 cctgcaaggc gggcatactg cctgaccttg agggccacag caccatctgc cagcgtgccg
     4441 gtccagggcc tgccagccga cacaccgaca aaagatactt ccagttgccc gttgaattcg
     4501 gccggatttt tcaccgcctg aatcaccagc acctgccatt tgagttcacc ggtcttgagc
     4561 agttcggcct gcaagccgcg aacggccaac gcacccgcgt tcaccccggt atctgctgcc
     4621 ggaatcaatt gctcaaaaaa acccaggtca tctttcaggc gctggttgtc cagcgccaat
     4681 tgcttgttca gtgcggccaa tttctcttgc gccacctttt cggtcatcaa cacagtatcg
     4741 gcagtgttgg cgatggactg cgccttgtcg cgttcaacgg ttaccgcatc gagctgggtc
     4801 ctcagggcat caagttgggt ctgcgcctgc tgcaactgct ctttggtgcc gtgatccagc
     4861 ccggcaattt cccggccaaa ctcaaacgcc cacaaggcca cggcagcgca aaagcctgcc
     4921 accgtcgcca gcatcaccca gcgaaccggc catggcaggg cactgcgcac cgccatgcgc
     4981 ggggcgctga cggtcaggcg gcgcagcaac aatcgaagtc gcatcagatt ggtcctgaaa
     5041 gcaccatgca aaaagccacc agaacttgca ttcgggtggc tgaaatgcag agaagcgaga
     5101 aaatattaac gcttgctgaa ctgcttgcga cggcgggcac cgtgcaaacc gaccttttta
     5161 cgttccactt cacgcgcgtc acgggtaacg aaaccagctt tgctcagatc gggcttgagc
     5221 gtggcatcgt agtcaatcag ggcgcgggta atgccatggc gcaccgcgcc ggcctgaccc
     5281 gactcaccgc cacctgccac attgaccatc acgtcaaagg tttcggcgtg attcgtcagc
     5341 aacagcggct gcttgcagat catgatcgag gtggcgcggc caaaaaagtt ttcaatcgcc
     5401 ttgccattca ccacgatctg gccagagccc tttttcagga aaacacgggc cacgctggat
     5461 ttgcgacggc cggtaccatt gttccattca ccaatcatca atatgctcct taaagtacca
     5521 acactttggg ttgctgggca gtgtgcgggt gctctgcacc accgtacacc ttgagcttct
     5581 tgatcatggc gtagcccaaa gggcctttgg gcagcatgcc cttgacggct ttttccaaag
     5641 cgcggccagg gaatttggct tgcatgtcac ggaagttggt agaactgatg ccgcccggat
     5701 aacccgagtg gctgtaatac atcttgtcga gcgacttggc accggtcacc cgcaattggg
     5761 ctgcgttggt gacgatgatg aagtcgccgg tatcgacgtg aggcgtgtaa atggccttgt
     5821 gtttgccgcg caaacggaga g
//
LOCUS       DAOSLL010000267         5839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12375_length_5839_cov_4.432054, whole genome shotgun sequence.
ACCESSION   DAOSLL010000267 DAOSLL010000000
VERSION     DAOSLL010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12375_length_5839_cov_4.432054"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PLB25_12110"
     CDS             complement(<1..487)
                     /locus_tag="PLB25_12110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42357.1"
                     /translation="MDTHDQLQVLTPRGLQHFVSLQADAATAVTRRFYTTHGSHYARY
                     GERGHEACREDLAFHLEFLRPVLEFGLLQPMVDYLCWLDSVLAGRGIPTEHLTQSLDC
                     LGEYFAQAMEPEDGAVVVAAILAARTKFGQSRKLALIAPELPEAWPRSAAFEAALLTG
                     DQ"
     gene            complement(993..1154)
                     /locus_tag="PLB25_12115"
                     /pseudo
     CDS             complement(993..1154)
                     /locus_tag="PLB25_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868674.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
     gene            complement(1275..3065)
                     /locus_tag="PLB25_12120"
     CDS             complement(1275..3065)
                     /locus_tag="PLB25_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOE42358.1"
                     /translation="MSYLAPLKDMLFNIRYLAGIEQIAQLPGMEDAGFDTAQAVLEEA
                     AKFNEGVLSPLNWVGDQNPTSWKDGVVTATPGFKEAFRQFAEAGWQGLQHPVAYGGQG
                     LPKTIGSAVGEMQNSANMSFALLPLLSDGAIEALLTAGSDELNATYLDKLVSGQWTGT
                     MNLTEPQAGSDLAAVRTKAEPQPDGTFKIAGTKIFITWGEHDIAENIIHLVLARVVGA
                     PEGVKGISLFVVPKFIVNQDGGLGARNDVQCVSIEHKMGIQASPTCVLQFGDHGGAVG
                     YLVGQENRGLEYMFIMMNAARYGVGVQGIAIAQMAYQKAAQFAKDRVQSRPVDGSLKA
                     AGPIIHHPDVRRMLMTMRAYTEGCRALSSVAAAAYDAAHHHPDARTRDENETFYEFMV
                     PLIKGYSTEMSQEVTSLGVQVHGGMGFIEETGCAQYYRDAKILTIYEGTTAIQANDLV
                     GRKTSRDGGQTAKALAVQIQTTEAALNSSGTPDALAMAKRLAAARLAFLDVVDFVAGN
                     TKAHPNDVFAGSVPYLMLTGNLMAGWQMARALLVAQEALARGEDVAFMQAKIITARFY
                     ADHLLTKAPGMRDAIVEGAACVTALALEAF"
     gene            complement(3169..4104)
                     /locus_tag="PLB25_12125"
     CDS             complement(3169..4104)
                     /locus_tag="PLB25_12125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HOE42359.1"
                     /translation="MTILVIAEHDNASLKPATLNTVTAALQCGAVVHVLVAGFNAGAA
                     ASAAAQVAGVAKVLHIDADHLAHGIAENVTAQILAIAAGYSHILFAATASGKNIAPRL
                     AAKLDVAQISDITKVISADTFERPIYAGNAIATVQSTDGTKVITVRTTGFDAAAQSGQ
                     APVAIETVATASVPGSTVYVGSEIAKNDRPELTAAKVIVSGGRALGSNEKFMAVMTPL
                     ADKLGAALGASRAAVDAGYAPNDWQVGQTGKIVAPQLYVAAGISGAIQHLAGMKDSKV
                     IVAINKDPEAPIFSVADYGLEADLFIAVPELVAAL"
     gene            complement(4237..4986)
                     /locus_tag="PLB25_12130"
     CDS             complement(4237..4986)
                     /locus_tag="PLB25_12130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003819290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HOE42360.1"
                     /translation="MKVLVAVKRVVDYNVKVRVKSDNTGVDIANVKMSMNPFDEIAIE
                     EAVRLKEKGVATEIIAVSCGVTQCQETLRTAMAIGADRAILVETAEELQPLAVAKLLK
                     ALIDKEQPGLIIVGKQAIDDDCNQTGQMLAALADLPQVTCASKIVVADGKASITREVD
                     GGSETLSVSLPAVMTADLRLNEPRYVTLPNIMKAKKKPLQVVKPADLGVDIAARIKTL
                     AVNDPPKRSAGIKVPDVATLVDKLKNEAKVI"
     gene            complement(5169..>5839)
                     /locus_tag="PLB25_12135"
     CDS             complement(5169..>5839)
                     /locus_tag="PLB25_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOE42361.1"
                     /translation="GGELSLRKAFSNASRALLMFLGLLLALSAVGIDLTALSVLGGAI
                     GVGIGLGLQKLAANYVSGFVILAERSMRIGDSVMVDNFSGIITQINARYTVIRSLSGR
                     ESIVPNEMMITSRVENHSLADPKVWQSTIVSVGYESDVQQVMTLLTQAALAQARVLRD
                     PPPSAALSNFGADGLDFTLGFWIGDPENGSANIKSQVNLEVLRLLRENSIEIPFPQRV
                     IHQR"
BASE COUNT         1185 a         1740 c         1704 g         1210 t
ORIGIN      
        1 gctgatcacc ggtgagcagt gccgcctcaa aggcagccga tcgcggccag gcttcgggca
       61 actcgggcgc aatcagcgcc aacttgcggg attgaccaaa ctttgtccgt gctgccagga
      121 tcgctgcaac cacgaccgcg ccgtcctcag gttccatcgc ctgggcaaaa tactcgccca
      181 ggcagtcgag ggactgggtc aggtgttcgg tcgggatacc acgcccggca agcacgctgt
      241 caagccagca caggtagtcc accatgggtt gcaaaaggcc aaattccagc accggccgca
      301 ggaactcaag atgaaaggcg agatcttcgc ggcaagcctc atggccacgt tcgccatagc
      361 gcgcgtagtg ggagccatgt gtcgtgtaga agcgcctggt cacggcagtt gccgcatcgg
      421 cctgcaagct cacgaagtgc tgcaggccgc gcggcgtcaa gacttgcagt tggtcatgtg
      481 tgtccattga gtaatcctat ccgtgttccg acgcgaaaaa ccccgctcgt ggtgcaactg
      541 cccggccaaa ccagatgccg acatcggcat caacaccact cggtcccaaa tactgatttt
      601 ttcatggtag ccaccggcca gcggaaaacc aatcaggtcg atacctgagt cgggcgggta
      661 aaacacccga cgcggatccg ctcacaccag acccgctgag accggccaaa cgccaggatg
      721 cgacactgaa gaaggtgcaa agccatacgc tcgcaaacgg cacacccaca cacagtctcc
      781 agaccttgat ggccgaattg cgagcaacgg agggcaaaac ctgtcgcacc gctggcggtg
      841 tggctgaggc agccaatttc gcgatcaccc ctggcccaaa gcgacaacgt gctctcgaat
      901 tgatcggtca gagcaaaacg cagtcagacc accagacctc aatcaacggt gttttcattg
      961 aggaagaccg gctttttttg aaaatgtctt cagtccgatt gcgcgacagg cctggcgcca
     1021 aacgcaggcc cgacacccac cacgcctgca atgcactggg ctgtcagcag tttgggtttg
     1081 ctgatgatca acaacggcgc gccaatcact ggaaaaggcc gcttgttcag agctgcaggc
     1141 agtctggccg tgattcggcc actttatgca cgaaaatagc tgctagccct tatagtgatt
     1201 gcgctagcag ctacaaatac agtagcaaat tgcggtcatt tggacagtca gtgccaggtg
     1261 ctgacccaaa tggcctagaa cgcctcaagc gccagcgcgg taacgcatgc agcaccttcg
     1321 acaatcgcgt ctcgcatgcc gggagccttg gtcaacaaat ggtcggcata aaaacgtgcg
     1381 gtgatgatct tggcctgcat gaaggccacg tcctcacccc gcgccagcgc ctcttgtgcg
     1441 accagcaggg cacgtgccat ttgccagccg gccatcaggt tgcccgtcag catcaggtag
     1501 ggcacgctgc cggcaaacac gtcgttgggg tgcgccttgg tgttgcccgc gacaaagtcc
     1561 accacatcca gaaaggccag ccgggctgct gccaggcgct tggccatggc cagcgcatcg
     1621 ggcgtgccgc tgctgttcag ggccgcttcg gtggtctgga tctgcactgc cagcgccttg
     1681 gcggtctggc cgccgtcacg cgaggtcttg cgacccacca ggtcattggc ctgaatggcg
     1741 gtggtgccct cgtagatggt caaaatcttg gcatcgcggt aatactgggc acaaccggtt
     1801 tcttcgataa aacccatgcc gccatgaacc tgcacgccca ggctggtgac ttcctggctc
     1861 atttcggtgc tgtagccctt gatcagcggc accatgaact cataaaaggt ctcgttttca
     1921 tcgcgcgtgc gggcatccgg gtggtggtgc gctgcgtcat aagcagcggc ggcgaccgac
     1981 gacagggcgc gacagccttc ggtataggcc cgcatggtca tcagcatgcg cctgacatcc
     2041 gggtggtgaa tgatcggacc cgctgctttc agggaaccat cgaccgggcg gctttgcacc
     2101 cggtctttgg caaactgtgc ggccttctgg taggccatct gggcaatcgc aatgccttgc
     2161 accccaaccc catagcgggc cgcgttcatc atgatgaaca tgtattcaag gccccggttt
     2221 tcctgcccta ccagataacc aacggcacca ccgtggtcgc caaactgcag cacacaggtc
     2281 gggctggcct gaatgcccat tttgtgttcg atgctgacgc attgcacgtc gttgcgcgcg
     2341 cccaggccac cgtcctggtt gacgatgaac ttgggcacga caaacaggct gatgcccttg
     2401 acaccttcgg gcgcgccaac cacccgggcc agcaccaggt gaatgatgtt ctcagcgatg
     2461 tcatgctcgc cccaggtgat gaaaatcttg gtgccagcga tcttgaaagt accatctggc
     2521 tgcggctcgg ccttggtgcg cactgccgcc agatcactgc ccgcctgtgg ctcggtcagg
     2581 ttcatggtgc cagtccattg gccactgacc agcttgtcca ggtaggtggc gttgagttcg
     2641 tctgaaccgg ccgtcaacag cgcctcaatg gcaccgtcgc tgagcagcgg caacagggca
     2701 aaactcatgt tggcggaatt ctgcatttca cccactgccg agccaatggt cttgggcagg
     2761 ccctgcccgc cataggccac cgggtgctgc aagccctgcc agccagcttc agcaaattgc
     2821 ctgaacgcct ccttgaagcc cggcgtggcg gtgacaacac cgtccttcca gcttgtcggg
     2881 ttctggtcac caacccagtt cagcgggctg agcacgccct cattgaactt ggctgcttct
     2941 tccagcaccg cctgcgccgt gtcaaaaccg gcatcttcca tgcccggcaa ctgggcaatt
     3001 tgctcgatac cggctaagta cctgatgttg aacagcatgt ctttgagggg ggctaggtaa
     3061 ctcatggtcg tttctccgga gggttatgaa agggatggca aggttggcga aggggcaagc
     3121 aggcgaaaaa aaagggcatc gcagcggatg ccctgtggtg taactgcttt acagggcggc
     3181 aaccagttct ggcaccgcaa taaacaaatc ggcctccagc ccatagtcag ccacgctgaa
     3241 gatgggtgcc tcggggtctt tgttgatggc gacgatgacc ttgctgtctt tcatgccggc
     3301 caggtgctga atggcaccac tgatgcccgc tgcaacatag agctgcggtg cgacgatttt
     3361 gccggtctgt ccaacctgcc agtcgttggg cgcatagcct gcatcgacgg ccgcccggct
     3421 ggcacccaaa gcggcaccga gcttgtcggc cagtggtgtc atgacggcca tgaacttttc
     3481 gttggagccc agcgcccggc caccgctgac gatgactttg gcagcagtaa gttccgggcg
     3541 atcgttcttg gcaatttcac tgccgacata gacagtgctg cccgggacgc ttgcagtagc
     3601 tactgtttca atagctaccg gcgcttgtcc tgattgggct gcagcatcaa aacccgtggt
     3661 tcgaacggtg atgaccttgg tgccatcggt gctttgcacc gtggcgatgg cgttgccggc
     3721 gtagatcggg cgctcgaagg tgtcggcgct gatgaccttg gtgatgtcag agatttgcgc
     3781 cacatcgagc ttggccgcca gccgcggggc aatgttcttg cccgaagccg tcgcggcaaa
     3841 caggatgtgg ctgtaaccgg ccgcaatcgc gaggatttgg gcggtcacgt tttctgcgat
     3901 gccgtgcgcc agatggtctg catcaatgtg caaaaccttg gcaacaccgg caacctgtgc
     3961 tgccgcgctg gcagcggcac cggcattgaa accagccacc agcacatgaa ccacagcgcc
     4021 acactgcagc gcagcggtaa cggtgttgag ggtggcgggc ttgagacttg cgttatcgtg
     4081 ttcggcgatg acgaggatag tcatgaatga actcctgttg gaatgggtag gtctggtaaa
     4141 gatgtttcaa aaacttgggt cttgcggggc tgatctgcca ccttggtggc acagaacgca
     4201 tggatgtccg gatcaactcc cgaaagaccg caacagctag atgactttgg cttcgttttt
     4261 gagtttgtcc accagcgttg ccacatcggg caccttgatg ccagcgctgc gcttgggcgg
     4321 atcattgacc gccagggtct tgatacgcgc ggcgatgtca acacccaggt cggctggctt
     4381 gacgacctgc agaggcttct tcttggcctt catgatgttg ggcagcgtca cataacgtgg
     4441 ctcgttgagg cgcaggtcag cggtcatcac agcgggcaag ctgacgctga gggtttccga
     4501 accaccatcg acttcacggg tgatgctggc tttgccatcg gcgaccacga tcttgctggc
     4561 acaggtgacc tgtggcaggt cggccagggc ggcgagcatc tggccagtct ggttgcaatc
     4621 gtcgtcaatg gcttgcttgc ccacaatgat caagccgggt tgttccttgt cgatcagcgc
     4681 cttgagcagc ttggccacgg ccaggggctg cagttcttct gcggtctcga ccaggatggc
     4741 gcggtcagca ccaattgcca tggcggtgcg cagggtttcc tggcactggg tgacaccgca
     4801 agacacagcg atgatctcgg tggccacgcc tttttctttc aggcggacgg cttcttcgat
     4861 ggcgatttcg tcaaacgggt tcatgctcat tttgacgttg gcaatatcta caccggtgtt
     4921 gtccgatttg acgcggacct tgacgttgta gtccactacc cgcttgactg cgaccaggac
     4981 tttcatgggt ttgtttctcc agacaataaa aaagttaagc aaccgacaca tcgcgcagtc
     5041 aacactctta ttgacgtgca cgtcaacttt ggtattctat gcggccaact ttgccagcct
     5101 cgtcgaaccg acgcgattct tccgcctcgg ctgaaaatag aacgatcgtg cttttattat
     5161 aggcatcctc agcgctggtg gattacccgc tgcgggaatg gtatctctat actattttcg
     5221 cgcagcagcc gcagcacctc cagattcact tgcgacttga tgttggcact gccgttttcc
     5281 gggtcgccaa tccagaatcc caaggtgaaa tccagcccat cggcaccaaa atttgacagt
     5341 gctgctgacg gcggcgggtc gcgcaggaca cgcgcctgtg ccagcgccgc ctgggtcaac
     5401 agcgtcatca cctgctgcac atcactttca tagccgaccg acacgatggt tgattgccag
     5461 actttggggt cggccagcga gtggttttca acccggctgg tgatcatcat ttcgttgggc
     5521 acgatcgatt cacgaccgct gagcgagcga atcacggtgt agcgggcatt gatctgggtg
     5581 atgatgccgc tgaagttgtc gaccatcacg ctgtcgccaa tgcgcatgct gcgctcggcc
     5641 agaatgacaa atcccgacac atagttggcg gccagttttt gcagccccag gccaatgcca
     5701 acgccaattg caccacccag caccgacagc gcggtcaggt caatgcccac cgcagaaagc
     5761 gccagcagca gcccgagaaa catcagcagg gcgcgtgacg cgttgctgaa ggccttgcgc
     5821 aggctcagtt cgccaccgg
//
LOCUS       DAOSLL010000268         5819 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12417_length_5819_cov_4.022727, whole genome shotgun sequence.
ACCESSION   DAOSLL010000268 DAOSLL010000000
VERSION     DAOSLL010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12417_length_5819_cov_4.022727"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(136..1533)
                     /locus_tag="PLB25_12140"
     CDS             complement(136..1533)
                     /locus_tag="PLB25_12140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HOE42362.1"
                     /translation="MVWLALSLGACASLPGTPPLASSALVAPAAWANAEVQATAAKSS
                     LLRWWQRFDDPLLDQLISQALAANNSVQGAQAALRQARALHDVAAAGLSPSLGSSASA
                     QRSRRSNTTDNSFQVGLDGNWDLDVFGANRSAVSASEAAAQASAASLGTVQVSIAAEV
                     ALDYIALRSAQERLGIAAANLASQRETLQLTQWRLQAGLVTSLEAEQARAATEQTAAQ
                     LPVLQISMAQIGHALAVLTGQPPAALALLLADAGRLPQAPGALALAFPAETLRQRSDV
                     QAAEFQLTAAAARVAQADAARLPSFKLGGSLGLSALTLGALTDGASVAAAVLASVSWS
                     LFDGGAAQAQGRAQRAAFDQARATYQATVLTALQEVEDALVALRFDRQRLLRLQNAFA
                     SADIAATLASQRYSSGLVDFQAVLETQRSRLSTQDGVASARAAVSADHVRLYQALGGG
                     WLPSDAEATALSATP"
     gene            complement(1667..2173)
                     /locus_tag="PLB25_12145"
     CDS             complement(1667..2173)
                     /locus_tag="PLB25_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42363.1"
                     /translation="MSNPFNATAAVSRPKAVFAPLQGEADTRPIDPAHEWQINQQFIA
                     MLNAYRPSGGLARAQELAARFSLQGTAQVNTLAGWIVHRQVVSLSWQGKIWLPLFQFN
                     HVNLCLLPGLATVMAELVVALDDWEIANWFSLPNPWLADCTPADTLLTAAPEVLNAAR
                     AERYVALG"
     gene            2424..2633
                     /locus_tag="PLB25_12150"
     CDS             2424..2633
                     /locus_tag="PLB25_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42364.1"
                     /translation="MNTMKGDVYLITPLDLSALGEHLGCCQHPHRHWFILHDLADAMH
                     GFVSARFVTTLGIIGLAVLFADWLI"
     gene            2643..>5819
                     /locus_tag="PLB25_12155"
     CDS             2643..>5819
                     /locus_tag="PLB25_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOE42365.1"
                     /translation="MRSKLFGLIPEAPETLCHMLDGARGAVQVPIRSEIFGLQRFAQH
                     GRSLAETHSASKSSRASNFFPRITDNIAMLREAHDLIGVQASSGYDISPAAEWLLDNF
                     HLVQAQLKEISDGLPRSYFRSLPMLQDEPLAGLPRVYGVAWAFVAHTDGAFDEPLLVQ
                     YLSAYEATRTLNLSEMWALPTTLRVVLIENLRRLAERVATNKGAREAANLCFDHIDRY
                     PLAALDALLALLKARGVGSAFLVQLALRLQDHHALQDSADQAELQAWLQQALPDLASL
                     QTSQAADQAADNLSMSNAITSLRAIGDADWPGIIARVSTLMQLMLGSAVFAAEDTLTR
                     DLSLHAIERLARNSGQSEVAVAQVLLNQMAPASQADNPCSVPTYWLCGAGRPAMLRSL
                     GQDPRAARAWAFVTRYLALPLYLGILMVCLGLLFWLLLQHSPQMSSWLLLLLGALAIF
                     PASEAVVAVTNRIISESARPQHLPRLALADGIPPGQRVMVVIPGMLTAPGSAHDLAHR
                     LHLHYLANPEREAQFALLTDWGDAETAHTAKDGGYLADAVAHIEALNASLPAQSALGA
                     PRFIVLHRPRQFSQTEQRWIGWERKRGKLEALVAALATGHSTAFTDCGEASRMAADVR
                     YIVTLDADTQLPPGSLRELVGVAAHPYNQPQLDATGRRVVAGYGILQPRVAAPLPERN
                     EATLYHWLFAGQCGMDTYSAASSEVYQDLFAQGTFTGKGLLNVQAMHAVLHDRLPEGQ
                     VLSHDLLEGSVARCATVTDIALIEESPFHADVAASRVHRWTRGDWQLLPFLTQPGRFG
                     LNAVNCWKMFDNLRRSLVAPMSLGLLLLALASQAVSPWLALLLVLAAFSAGPVLGAAA
                     GLSLHRSDTVQGHFYREALRDLLRAVLGGVWQMAQLLQQSLLNLDAIGRALYRMRFSH
                     RHLLQWTTAAQAQAQAKSALTDVARQHWIEPVVALLLLAGLLARGSSTPWLAVAVCLL
                     WAGSPVWTWWVSRACFFDPADRLTPADQFYLAGIARDTWRLFERCVGPLDRHLPPDNL
                     QISPYDMLAHRTSPTNIGMYLLSVACARQ"
BASE COUNT          973 a         1939 c         1852 g         1055 t
ORIGIN      
        1 ctggcggcgg tgactgcaca ggcggcacac cggggattgg tgccacgccg ggggcgacaa
       61 caggggtcgt cggcagggct gtggcaggtg tcggtgttgt tgggttttcg gaggtcattg
      121 gttcggtgtc ctgggttatg gcgtggccga caacgctgtg gcttcggcat ctgacggcag
      181 ccagccgcca ccaagcgcct ggtacaagcg cacatggtcg gcactgaccg cagcgcgggc
      241 actggccacg ccgtcttgcg tgctcagtcg gctgcgctgg gtttccagca cggcctggaa
      301 gtccaccagg ccgctgctgt agcgctggct ggccaacgtg gcggcgatgt cggcactggc
      361 aaacgcgttt tgcaggcgca gcagccgttg gcgatcaaag cgcaaggcca ccagtgcgtc
      421 ttcaacctct tgcaaggcgg tcagcaccgt ggcctggtag gtagcgcgag cctggtcaaa
      481 agccgcccgt tgcgcgcgcc cttgcgcctg cgctgcgccg ccatcaaaca acgaccatga
      541 cacgctggcc aacactgctg ctgccaccga ggccccatcg gtaagcgccc ccagcgtcag
      601 ggcactcaaa cccagcgacc cgccgagctt gaaactgggc agcctcgccg catcagcttg
      661 cgccacgcgg gcggcggcgg cggtcaactg aaattccgct gcctgcacat cgctgcgctg
      721 gcgtaaggtc tctgccggaa acgccagcgc caaagcgcct ggtgcctgcg gcaaccggcc
      781 agcgtctgcc agcagcagcg ccaaagccgc tggcggctgc ccggtcagca ccgccaacgc
      841 gtggccgatc tgggccatgc tgatttgcag cacaggcagt tgcgcggcgg tttgctcggt
      901 ggcggcgcgg gcttgttcgg cctccagtga agtcaccagc ccggcctgca agcgccattg
      961 ggtcaattgc agcgtctcgc gctggctcgc caggttggcc gcggcaatgc cgagccgctc
     1021 ctgcgcactg cgcagcgcga tgtagtccag cgccacctct gcggcaatcg acacctgcac
     1081 cgtgcccagg ctggccgcac tggcctgcgc ggcggcctcg ctggcgctca cggcgctgcg
     1141 gttggcccca aagacatcga gatcccagtt gccatccagg ccaacctgaa agctgttgtc
     1201 agtggtgttg ctgcggcggc tgcgctgcgc cgaggccgaa ctgcccagcg acggcgacaa
     1261 gccagccgcc gccacatcgt gcagcgcgcg cgcctggcgc agtgccgcct gtgcgccctg
     1321 aacgctgttg ttggcggcca gtgcctggct gatcaactgg tccagcagcg ggtcatcaaa
     1381 gcgctgccac cagcgcagca gcgaggactt ggccgctgtg gcttgaacct cggcattcgc
     1441 ccatgcagca ggcgcaacca aggccgacga tgccaacggc ggcgtgccgg gcagcgacgc
     1501 acaagcccca aggctcagcg ccagccagac caccaggccc gcttgccccc aggttgcaag
     1561 cgcttgcccg atcagatctg gttgcgtttg ttttacaagc cttttaggcc tgtagccctt
     1621 atggaatatg cgcaagcagc tattgttata gaagcgcata ggcgactcaa cccagcgcca
     1681 cgtaacgctc ggcgcgggcc gcattgagca cttcgggcgc agccgtcagc agcgtgtcgg
     1741 ccggcgtgca gtcggccagc caggggttgg gcagcgagaa ccagttggcg atttcccagt
     1801 catccagggc taccaccagc tccgccatca ctgtcgcaag cccgggcagc aagcatagat
     1861 tgacatggtt gaactgaaac agcggcagcc agatcttgcc ctgccacgac aagctgacca
     1921 cctggcggtg gacgatccag cccgccagcg tgttgacctg ggcagtgccc tgcaagctaa
     1981 atcttgccgc aagctcttgc gctcgcgcca ggccaccgct gggccggtag gcgttgagca
     2041 tggcaatgaa ctgctggttg atctgccact cgtgcgccgg atcgatcggc cgggtgtcgg
     2101 cttcgccctg cagcggagca aacaccgctt tgggtcggga tacggcagca gttgcattga
     2161 acgggtttga catggggtct ccaaagaggt gcgtcagcgg ctcggccccg gcaaattcgc
     2221 ctggggctgg cgtaacacaa cgctggaagt taaggccgca cagtgtgatg gtctgtgcgc
     2281 ttgttaacgc aagcaatgtt cgcaagcgca cagaccctgc cagagcgctc gcccacactt
     2341 gccgccatgg cgcacaccag cgtcagcagg cctgtcttga cggccccgga acaccacttc
     2401 aactcaatct caaaggaatc catatgaaca ccatgaaggg cgatgtgtat ctgatcacgc
     2461 cactggactt gtccgccctg ggtgaacacc tgggctgctg ccagcacccg caccggcact
     2521 ggttcatcct gcacgacctg gccgatgcga tgcacggctt tgtttcggcg cgctttgtga
     2581 ccacgctggg catcatcgga ctggctgtcc tgtttgccga ctggctgatc tagggcgcgg
     2641 gtgtgcgcag caaactgttt ggcctgattc ctgaagcccc ggaaaccttg tgccatatgc
     2701 ttgatggcgc acgtggtgcg gttcaggtgc cgattcgctc cgaaatattt ggtttgcagc
     2761 gctttgccca gcacggccgc agtctggccg aaacccacag cgccagcaaa tcgtcgcggg
     2821 catccaactt tttcccgcgc atcaccgaca acatcgccat gctgcgcgaa gcgcacgact
     2881 tgatcggggt gcaggccagc agcggctatg acatcagtcc ggcggccgaa tggctgctgg
     2941 acaactttca cctggtgcaa gcccagctca aggaaatcag cgacggcctg ccgcgcagct
     3001 atttccgcag cttgcccatg ttgcaagacg agccactggc cgggctgccg cgcgtttatg
     3061 gcgtggcctg ggcgtttgtg gcacacactg acggggcctt tgacgagcca ctgctggtgc
     3121 agtacctgag tgcctatgag gcaacgcgca cgctcaatct gagcgaaatg tgggcactgc
     3181 ccaccacctt gcgggtggta ctgatcgaaa acctgcgtcg actggccgag cgcgtcgcca
     3241 ccaacaaggg tgcccgcgaa gcggccaacc tgtgctttga ccacatcgac cggtatccgc
     3301 tggcagctct tgatgcgctg ctggcgctgc tgaaagcgcg tggtgtcggt agcgcctttc
     3361 tggtgcaact ggcgctgcgc ctgcaagacc accatgccct gcaggacagt gccgaccagg
     3421 ccgaactgca ggcctggctg caacaggcgc tgcccgacct ggccagcttg cagaccagcc
     3481 aggcggcgga ccaagccgcc gacaacctga gcatgagcaa cgccatcacc tcgctgcgcg
     3541 ccattggcga tgccgattgg cccggcatca tcgcgcgcgt cagcacgttg atgcagttga
     3601 tgctgggttc tgccgttttt gccgctgaag acactctgac ccgcgacctc agcctgcacg
     3661 ccattgagcg actggcccgc aacagcgggc agtctgaagt cgcggtggcg caggtgctgt
     3721 tgaaccagat ggccccggcc agccaggccg acaacccgtg cagcgtgcca acctattggc
     3781 tttgcggcgc gggccggccc gccatgttgc gctcgctcgg gcaagacccg cgtgcggcgc
     3841 gtgcctgggc ctttgtcacc cgttacctgg ccttgccgct gtacctgggc atcttgatgg
     3901 tgtgcctggg tttgctgttt tggctgctgc tgcaacacag cccgcagatg tcgagctggc
     3961 tgctgttgct gctgggtgcg ctggcgattt ttccggcctc cgaggcggtg gtggcagtca
     4021 ccaaccgcat catcagcgaa tcggcgcgcc cgcagcacct gccacggctg gcgctggcag
     4081 atggcatacc acccgggcaa cgtgtcatgg tggtcattcc aggcatgctg accgcgccag
     4141 gctcggcgca cgacctggcc caccgcctgc atttgcatta cctggccaac cccgagcgag
     4201 aagcccagtt tgccctgctg accgactggg gcgatgccga aacggcgcac accgccaaag
     4261 acggcggcta tctggcagat gccgtggcac atatcgaagc actcaatgcc agcttgccag
     4321 cgcagtcggc tctgggtgca ccgcgcttca ttgtgctgca ccggccgcgc cagttcagcc
     4381 agaccgagca gcgctggatt ggctgggaac gcaaacgtgg caagcttgaa gccctggtcg
     4441 ccgccctggc gaccggccac agcacggcat ttaccgactg cggcgaggcc tcccgcatgg
     4501 cggcggacgt gcgctacatc gtcacccttg atgccgatac ccaactgccg cccggcagtc
     4561 tgcgcgaact ggtgggtgtc gcggctcacc cctataacca gccgcaactc gatgccactg
     4621 gccgacgcgt ggtagcgggc tatggcattt tgcaaccccg ggtggccgcc ccgctgcccg
     4681 agcgtaacga agccaccctg taccactggc tgtttgccgg gcagtgcggc atggatacct
     4741 acagcgccgc cagctccgag gtttaccagg acctgtttgc ccagggcact ttcaccggca
     4801 aaggcttgct caacgtgcag gccatgcacg cggtgctgca cgaccggcta cccgagggcc
     4861 aggtgctcag ccatgatttg cttgaaggtt cagtcgcccg ctgcgctacc gtgaccgaca
     4921 ttgcgctgat cgaagagtcg ccgtttcatg ccgatgtggc ggcctcacgg gtgcaccgct
     4981 ggacccgtgg tgactggcag ttgctgccgt ttctgaccca gcccggccgc tttgggctca
     5041 acgccgtcaa ctgctggaag atgtttgaca acctgcgccg ctcgctggtg gcaccgatgt
     5101 cactgggctt gctgctgctg gcgctggcca gccaggccgt gtcgccatgg cttgcgctgc
     5161 tgctggtgct ggcggccttt tcggccggtc cggtgctggg tgcggcagcc gggctttcgc
     5221 tgcaccgcag cgacaccgtg caggggcatt tttaccgcga ggcgctgcgt gacctgctgc
     5281 gcgcggtgct tggcggggtt tggcagatgg cgcaactgct gcagcagtcg ctgctgaacc
     5341 tggatgcgat tggccgcgca ctctaccgca tgcggttcag tcaccgccat ttgctgcaat
     5401 ggaccaccgc cgcacaagca caagcccagg ccaaatctgc cttgacagac gtggcccggc
     5461 aacattggat tgagccagtg gtggccctgc tactgctggc cggcctgctg gccaggggca
     5521 gcagcacgcc atggctggcc gtcgcggtgt gcctgctgtg ggctggctcg ccagtctgga
     5581 cctggtgggt cagccgcgcc tgcttttttg acccggcaga ccgcctgaca ccggccgacc
     5641 agttctatct ggccggtatt gcgcgtgaca cctggcggct gttcgagcgc tgcgtggggc
     5701 cacttgaccg ccacctgcca cctgacaacc tgcagatctc gccctacgac atgctggccc
     5761 accgcacctc gcccaccaac atcggcatgt acctgctcag cgttgcctgc gcccgtcag
//
LOCUS       DAOSLL010000269         5786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12501_length_5786_cov_4.313558, whole genome shotgun sequence.
ACCESSION   DAOSLL010000269 DAOSLL010000000
VERSION     DAOSLL010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5786
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12501_length_5786_cov_4.313558"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1706
                     /locus_tag="PLB25_12160"
     CDS             <1..1706
                     /locus_tag="PLB25_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004555622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOE42366.1"
                     /translation="SSTASQAQSPLQAEPVDFVQVPESQQADQWAQPPKAHESAHWVT
                     RTALCVEVRDPRRASGPKAEAVGEKSGVIYVFMPPLERLEYYLDLLAAIEATAQELGV
                     QIVLEGYPPPRDPRLKLLSVTPDPGVIEVNIHPVTNWKELVYNTEFLYNAAFESRLSA
                     EKFMTDGRHTGTGGGNHFVMGGATPADSPFLRKPELLASLLLYWHNHPSLSYLFSGMF
                     VGPTSQAPRVDEARNDQLYELEIAIEQIYKNREIHGQSMPPWLVDRTLRNILIDATGN
                     THRSEFSIDKMYSPDSSTGRLGLLELRAFEMPPHPHMSSVQQLLLRALVARFWKAPYQ
                     VPATRWGTELHDRFMLPKFIEMDFHDVMAEMRGAGYAFDESWFAPHVEFRFPLIGSVC
                     AAGIELTLRNALEPWHVMGEEGAPGGTARYVDSSLERIEVRVTGLNQSRYVVTCNGQA
                     MSLQPTGVVGEYVAGVRYKAWNPPSSLHPSIGVHAPLTFDIVDTWVKRSMGGCQYHVA
                     HPGGLSYQALPVNANEAESRRLSRFTAGGHTPGVLVVPPATINVPASREFPFTLDLRR
                     A"
     gene            complement(1862..2587)
                     /locus_tag="PLB25_12165"
     CDS             complement(1862..2587)
                     /locus_tag="PLB25_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HOE42367.1"
                     /translation="MTDTPTEMTPTNSTTELAAPIDKAVLSTDDPVKQAGPTTGASPK
                     KDRFAAVAPVLEKLFELYPHLFGQRFVPLKLGVFQELLAAHPTELKRDTLKAALAVHT
                     RSTRYLQSVAVGTKRHDLSGQPVDDVAPEHVFLSIVELHQRRQARTSDDLLPKLQRQL
                     VTAYAASGLSRQDYLSRLPALADSISAVLDEAMVEVDQQRARQAALVKAYQSSGKAVN
                     DFADDLGMDAARLSAVLKQAGVI"
     gene            2722..5343
                     /locus_tag="PLB25_12170"
     CDS             2722..5343
                     /locus_tag="PLB25_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HOE42368.1"
                     /translation="MHYRAAAGHFDEVRGEASAGTSSRTDNAPFFGAVSADGARAWNQ
                     FFQQLTSDTANDMEQRSSSLARQVRDNGITYNVYADENGPQRPWSLDLFPLIIDPKSW
                     QRIEAGVLQRVRLLEHVMTDVYGAQDLLRSGFLPPALVQGHPGYLRAMHGVKPVGGRH
                     LHIAGFDLARGPDGNWWVVGQRTQAPSGLGYLLENRLAISAQFAPAFETLRVQRLAGT
                     YRALMDGLRATSPMGQDAHLVLLTPGPYNETYFEHAYLARYLGLTLVEGSDLLVRDEK
                     VFLKTLKGLMPVHGMLKRVDDQYMDPLELRADSTLGVPGLLQAIRAGNVLVANTPGSA
                     FLESPALLGFLPALSRHLFDEDLSLPALPTWWCGERSAMLDALPRLRECAIKPTYPGS
                     ASHASFDAVLGARQDARSLDEWAGRMLRHPEEHTVQAYTPLSQLPTWQIGNPGTGAAG
                     SLLARSVMLRVFAVSDGSHHWRVLPGGLARVASVGQEITSMQRGGSSADVWALTDGDL
                     DNSPLLQPHLTPESLMQRKRLVTSRAAENLYWLGRYTERTENALRLARLTLESLHGEE
                     QSSPPLLAWLGQMALANTLVLPGVPTPVQAPRVFERSLIDSLGSTSGATSVGYYLRSL
                     KIAASSVRERLSQEHWRFITRAEEELFARFAEHRRFGERSTLNALQILKETGEHMAAI
                     TGAQTDRMTRDDGWRLLSIGRHLERLAFLSSALSRALVCGSLESEGGFDAMVELFDNA
                     ITFHAQFQQSRDMAALVDLLVIDRDNPRSVSWVAHTLRGRLAKLAGSEPTELCALALK
                     IPNPNSWGLAQLCELTPDLTSPYQDSDSTSAASPRYALLRDVLYQCTAAAHAVSEEIS
                     VTYFTHSGLANRSVGTS"
     gene            5340..>5786
                     /locus_tag="PLB25_12175"
     CDS             5340..>5786
                     /locus_tag="PLB25_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase N-terminal domain-containing
                     protein"
                     /protein_id="HOE42369.1"
                     /translation="MRLQITHETVYDYSPAVETAQHMVYLTPMHCSTQQLLSHRLIVN
                     PQPTQISVMRDVFGNTRNFFSLQVAHRELCVVAVSEVLTQSRPLPDSELVWEHAREGF
                     RYRAGAAFDAANEFVFASPMVPRQGEFAHYARPSFAPGTGLLAAATD"
BASE COUNT         1068 a         1807 c         1802 g         1109 t
ORIGIN      
        1 actcgtcaac cgccagccag gcgcaaagcc cgttgcaggc cgagccggtc gactttgttc
       61 aggttcctga gtcccagcaa gcggaccagt gggcgcagcc acccaaggcg cacgagtcgg
      121 cccactgggt cacccgcacc gcgctgtgcg ttgaagtgcg cgacccgcgc cgtgccagcg
      181 gccccaaggc cgaggcggtc ggtgaaaagt cgggggtgat ttacgtcttc atgccacctc
      241 tggagcggct ggagtattac ctggacctgc tggccgcgat tgaagctacg gcgcaagagt
      301 tgggcgtgca aatcgtgctt gaaggctacc cgccaccacg tgacccgcgc ctgaaactcc
      361 tgagcgtgac acccgacccc ggcgtgatcg aggtcaacat tcacccggtg acgaactgga
      421 aagagctggt ttacaacacc gagtttttgt acaacgcagc gtttgagtcg cgtctgagcg
      481 ctgaaaaatt catgaccgat ggtcgccaca ccggcaccgg tggtggcaac cactttgtga
      541 tgggcggcgc cacaccggca gacagcccgt ttttgcgtaa acccgagctg ctggccagcc
      601 tgctgctgta ctggcacaac catccaagcc tgagttattt gtttagcggc atgtttgtcg
      661 gcccaaccag ccaggccccg cgtgtcgatg aggcccgcaa cgaccagttg tacgagctgg
      721 aaatcgccat cgagcagatt tacaagaacc gtgaaatcca tggccagtcg atgccgccgt
      781 ggctggtgga ccgcacactg cgcaacatcc tgatcgatgc caccggcaac acccatcgca
      841 gcgaattttc gattgacaag atgtactcgc ccgactccag caccggccgt cttggcctgc
      901 tggaactgcg tgccttcgag atgccgcccc acccgcacat gagcagcgtg caacagttgc
      961 tgctgcgggc attggtggcg cgcttctgga aagcacccta ccaggtgcca gccacccgct
     1021 ggggcaccga gctgcacgac cgcttcatgc tgcccaagtt catcgagatg gacttccatg
     1081 acgtgatggc cgagatgcgt ggcgccggtt atgcgttcga tgagagctgg tttgcaccgc
     1141 atgtcgaatt ccgcttccct ttgattggat cggtctgcgc cgccggtatt gaattgacgc
     1201 tgcgcaacgc cctggagccc tggcacgtga tgggtgaaga aggagcgccc ggtggcacgg
     1261 cgcgctatgt cgattcgtcg ctggaacgca ttgaggtgcg ggtgaccggc ctgaaccaaa
     1321 gtcgctatgt ggtcacctgc aacgggcaag cgatgtcttt gcaacccacc ggcgtggtcg
     1381 gcgagtatgt cgccggtgtg cgctacaaag cctggaaccc gcctagcagc ctgcacccga
     1441 gcattggggt gcacgcaccg ctgacctttg acattgttga cacctgggtc aagcgctcaa
     1501 tgggcggctg ccagtaccac gtggcgcatc cggggggcct gagctaccag gcgctaccgg
     1561 tcaacgccaa cgaagccgag agccggcgac tgtcgcgctt tactgctggc gggcacacgc
     1621 cgggtgtgtt ggtggtgcca ccggcaacca tcaatgtgcc agccagccga gagttcccgt
     1681 tcacgctgga tttgcggcgg gcctgaaaac cgggcaaaaa agcaatgcca ccgtggctgc
     1741 cgggcgctgc cctgcgccat cggcctggtg acccgctcgg gacttgaccg gcacaggcaa
     1801 tcggctcttg ccgctcgcgc aagcgtttgc cagggttacc ggcggggcgc gaccgggcgc
     1861 atcaaatcac cccggcttgc ttgagtaccg cgctcagtcg ggccgcatcc atgcccagat
     1921 catcggcaaa atcattcacc gccttgccac tgctttgata ggctttgacc aaagccgcct
     1981 ggcgggcacg ctgctggtcc acctcaacca tggcttcatc aaggactgca gagatgctgt
     2041 ctgccagcgc tggcaggcgg ctcaggtagt cctggcgcga cagaccactg gctgcgtaag
     2101 cggtcaccag ttggcgttgc agcttgggca agaggtcgtc agatgtgcgg gcctggcggc
     2161 gctgatgcaa ctcaacaatc gacaagaaaa catgctccgg ggccacatcg tcaaccggct
     2221 ggccagacaa atcgtggcgc ttggtgccga ctgccacact ttgcaggtaa cgggtggagc
     2281 gggtgtgtac cgccaaggca gccttgagcg tgtcgcgctt gagctcggtc gggtgggcgg
     2341 ccagcagttc ctggaaaacc cccagcttaa ggggcacaaa gcgttggcca aacaagtggg
     2401 gatagagttc aaacagcttt tccagcaccg gcgcaacggc tgcaaaccga tcttttttag
     2461 ggcttgcacc agtcgttggc ccggcctgct tgacggggtc gtcggtgctc aaaactgcct
     2521 tgtcgattgg ggcggccaac tcggtcgtgc tgttggtcgg ggtcatttct gttggggtat
     2581 cggtcatggc ttggatcagt tggattaacc cgtgattgtc atccaaagcg ctacttcgct
     2641 ggcatactct gaacaatgaa tcaagcccaa tctctgcatt tgaacttgcg ccctcctgcc
     2701 aacagcattg cagagctggc catgcactac cgtgccgccg ccggacactt tgatgaggtg
     2761 cgtggcgaag cgagcgccgg taccagcagc cgaactgaca acgctccttt ttttggagct
     2821 gtcagcgcag atggggcaag ggcctggaat caatttttcc agcaattgac gagcgatacg
     2881 gcaaacgaca tggagcagcg cagcagcagt ctggcgcgcc aggtgcgtga caacggcatc
     2941 acctacaacg tctatgccga tgaaaatggc ccacaacggc cctggtcact ggacctgttt
     3001 ccgctgatca tcgacccgaa aagctggcag cgcatcgaag ccggcgtgct gcagcgggta
     3061 cgcttgctcg agcacgtcat gaccgatgtg tatggcgcgc aagacttgct ccggtccggt
     3121 tttttgccac ccgcgctggt gcagggccac cccggttacc tgcgtgccat gcatggggtc
     3181 aagccggtgg gcggccgcca cctgcacatc gccggctttg acctggcacg cgggcctgac
     3241 ggcaactggt gggtggtcgg acaacgcacc caggcccctt ccgggctggg ttacctgctg
     3301 gaaaaccggc tggcgatttc ggcgcagttt gcgccagctt ttgaaacgct gcgggtgcag
     3361 cgcctggcgg gcacctaccg cgcgctgatg gatggtttgc gcgccaccag cccgatgggg
     3421 caggacgcgc acctggtgct gttgaccccc ggaccctaca acgaaaccta tttcgagcac
     3481 gcctacctgg cgcgttacct ggggctgacg ctggtcgaag gcagtgacct gctggtgcgg
     3541 gacgaaaaag tgttcctcaa gaccctcaag ggtttgatgc cggtgcatgg catgctcaag
     3601 cgggttgatg accagtacat ggatccgctg gaactgcgcg ccgactccac tttgggggtg
     3661 ccggggctgc tgcaggcgat tcgggccggc aatgtgctgg tggccaatac gcctggctcg
     3721 gcgtttctgg agtcacccgc cttgctgggc tttttgccgg ccttgtcgcg tcatctgttt
     3781 gacgaagacc tgagcctgcc ggctttgcct acctggtggt gtggcgaacg cagtgccatg
     3841 ctcgatgccc tgccgcgcct gcgtgaatgt gccatcaagc caacctatcc gggctctgcc
     3901 agtcatgcca gttttgatgc cgtgctgggg gcccggcaag acgcacgctc gctcgacgaa
     3961 tgggccggtc gcatgttgcg ccatccggaa gagcacacgg tgcaggccta cacgccgctg
     4021 tcgcaattgc cgacctggca gattggcaac ccaggcaccg gcgcagctgg cagtctgctg
     4081 gccagatcgg tcatgttgcg ggtgtttgcg gtatcggacg gcagccatca ctggcgtgtc
     4141 ttgcccggcg ggctggctcg ggtggccagc gtcgggcagg agatcacctc gatgcagcgt
     4201 ggcggcagca gtgccgatgt ctgggcgctg accgatggcg atctggacaa ctcgcccctg
     4261 ctgcagccac acctgacgcc tgagagcctg atgcagcgca agcgcctggt caccagccgg
     4321 gcggccgaga acctgtattg gctcgggcgc tataccgaac gtaccgaaaa cgccttgcgg
     4381 ctggcacgtc tgacgcttga gagcctgcat ggcgaggaac aaagctcgcc accgctgctg
     4441 gcctggctcg ggcagatggc gctggccaac accctggtgc tgcccggcgt gccgaccccg
     4501 gtccaggcac ccagggtgtt tgagcgctcc ctgatcgaca gtcttggcag cacctcgggc
     4561 gcgaccagcg tgggctacta cctgcgctca ctcaagatcg ccgcatcaag cgtgcgcgag
     4621 cgcttgtcac aagagcactg gcggttcatc acccgggccg aagaagaact gtttgcccgt
     4681 tttgccgagc accgccgctt cggtgaacgc tccaccctga acgcgctgca gattttgaaa
     4741 gaaaccggcg aacacatggc cgccatcacc ggtgcccaga ctgaccgcat gactcgcgac
     4801 gacggctggc ggctgctcag cattggccga caccttgagc gactggcgtt tttgtcgtcg
     4861 gccctgagcc gggcgctggt ctgcggcagc cttgagtccg aaggcggttt tgatgccatg
     4921 gttgagctgt ttgacaacgc catcaccttc catgcgcagt ttcagcaaag tcgtgacatg
     4981 gcggcattgg ttgacctgct ggtgatcgac cgtgacaacc cgcgttcagt gtcctgggtg
     5041 gcgcacacct tgcgcggccg actggccaaa ctggccggca gcgagccaac cgaactctgc
     5101 gccctggcgc tcaagatacc caacccgaac tcttgggggc tggcgcaatt gtgcgaattg
     5161 acgccagacc tgacatcgcc ctaccaggac agcgacagca catccgccgc cagcccgcgc
     5221 tatgccttgc tgcgcgacgt gttgtaccaa tgcacggccg ctgcgcatgc cgtatccgag
     5281 gaaatcagcg tcacttattt cacccattcg ggtctggcca accgcagtgt ggggacctca
     5341 tgagactcca aattacccac gaaacggtgt atgactacag cccggctgta gaaaccgcgc
     5401 agcacatggt ttacctcaca ccgatgcact gcagcaccca gcagttgctc agccaccggc
     5461 tgatcgtcaa cccgcaaccg acgcaaatca gcgtcatgcg ggatgtgttt ggcaataccc
     5521 gcaacttttt ttcgctacag gtcgcgcatc gggagttgtg cgtggtggcc gtcagcgagg
     5581 tgctgaccca atcccggccg ctgccggaca gcgagttggt ctgggagcat gcccgcgagg
     5641 gctttcgcta ccgggcaggc gcagcatttg atgcggccaa cgaatttgtc tttgcttcgc
     5701 caatggtgcc ccggcaaggc gaattcgccc actatgccag gcccagcttt gcgcctggca
     5761 ccggtctgct ggctgctgcg actgac
//
LOCUS       DAOSLL010000270         5775 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12533_length_5775_cov_4.565559, whole genome shotgun sequence.
ACCESSION   DAOSLL010000270 DAOSLL010000000
VERSION     DAOSLL010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12533_length_5775_cov_4.565559"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="PLB25_12180"
     CDS             <1..475
                     /locus_tag="PLB25_12180"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOE42370.1"
                     /translation="YKVNYTIAFDGETERTRDFLGAVNSVLNKDKDAASHDPFAWLHP
                     VNGYQGAAIQLGIWESLYEASDNALSLASGAFWANGIETGNQQIPKTQEYLAEFFAAV
                     DSADALLDKDVIVFRNGTYQDMITGDPIPEPGTLALLGAALVGWGLARRKPAPKV"
     gene            complement(658..1992)
                     /locus_tag="PLB25_12185"
     CDS             complement(658..1992)
                     /locus_tag="PLB25_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOE42371.1"
                     /translation="MNPSKLRASWHRALRQAFSLLPRTVRFAAYRSFVDCDPAPDPRL
                     HLKIAETRDELEACFGLLHDAYVASGFMKPHPSGLRVTLYHALPTTTTLCATFDGEVV
                     GTLSLIRESVMGFPLQSIFDLHSVREQGGMIAEVSALAVHPKFRKTGGSVLFPLMKFM
                     HDYCTGFFDTRHLVIAVNPNRIELYESLLLFDRLPQNTVDNYDFANGAPAVGATLDLH
                     HAAQKFRPIYTHKPLRRNLHHYFNELTLPNIELPARRYHTTNDPVMTPALLDYFFNQR
                     TQGFAELEDRKIHLLHAIYNLPAYRAVLPSLPEATTRAHLLRSFQRFSVKCPCMLRLN
                     PQAEQKSWQLKLVDVSENGFQVMCDDPLPLFTWGQVEIELGQAERSSLQAQAVWHKHN
                     AFGHFYAFEVGEPDLPWRRFVLALESGSTVNDLENASRFMEQQLTNVAGNLS"
     gene            1991..2470
                     /locus_tag="PLB25_12190"
     CDS             1991..2470
                     /locus_tag="PLB25_12190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42372.1"
                     /translation="MLSQSGELTPRHCKAVAAWIGFVLGILEIDTDIGGSLGILRVFE
                     ISGALSAKRGKLWHEQHVKHPIQGESRDSKGANPARVYKGHFHTFHVFLLMYSSSLQR
                     SELSQLTAWPYSRAIRSNAGRDAQKPDIVINLIAVSAILVMARVQKYSKMIATIFIK"
     gene            3266..3748
                     /locus_tag="PLB25_12195"
     CDS             3266..3748
                     /locus_tag="PLB25_12195"
                     /inference="COORDINATES: protein motif:HMM:NF024206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOE42373.1"
                     /translation="MTALMNAINNENVSLVGQLIRQGIDVNMLDANGDAPLVMAAYKG
                     NPDIVRMLLQAGADVKAVDPAMKATALHAAAYAGNADAAKLLVQYHIDIDKQGPMNGY
                     TALHDAIWQGHLETAKVLIDSGANLTLQSLHGETPLAFARQKHRSAIAALIERKLASK
                     "
     gene            complement(3806..4807)
                     /locus_tag="PLB25_12200"
     CDS             complement(3806..4807)
                     /locus_tag="PLB25_12200"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOE42374.1"
                     /translation="MNALLLDHPQHAFTLRMGAMPLPEPALGEIRVRLCAAALNPVDA
                     KLATGGNSAWTYPHIPGVDGAGWVDALGKGVTSFAIGDRVLAHFPLSKPGTLAEFALI
                     AERAVCEVPQTVSLLTAATLPCAAWTAWQALTRKLYLKSRQTMPTMLIEAAAGGVGGF
                     AVQMAKHLGATVIATCSDVNRKYVSELGADHVIDYRNEDVRESVLQLTGGRGVDAVLD
                     SLGGASGQRDLGLLAYAGSMACLINLPDVSKINVFRMAPSLHFITLGGAWLSGDQAAQ
                     LDLAKMGDEVLALLTTGAILPLPMTAVAFDAKAVTHALHQQLDGRVRGKQVVKIAAE"
     gene            5099..5773
                     /locus_tag="PLB25_12205"
     CDS             5099..5773
                     /locus_tag="PLB25_12205"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE42375.1"
                     /translation="MGLAAAQRLARAKADVAIVGRSQAKLDTALATIDGNASAFVADF
                     TDTASLAALVEQVGSIDHLVLVASSNAAFGPFSTTSAEALRRMLENKLLGYWQTLQAT
                     LPHLRRDGSVVMVTGAASRTAVSGMAGLAAVNGAITQLAQTLAKELAPLRVNVVSPGL
                     VDTPVYDQMPAEAKARMFADAAKSLPVGRVGTVDDIAMAIEFLIANSHTTGALIDVDG
                     GARMAG"
BASE COUNT         1379 a         1561 c         1613 g         1222 t
ORIGIN      
        1 ctataaggtc aattacacca ttgccttcga cggcgagacc gaacgcacac gggacttttt
       61 aggggcggtt aattcagtcc tgaacaaaga caaagatgcc gcttctcacg accccttcgc
      121 ttggttgcac ccagttaatg ggtatcaggg cgctgcgatc cagttgggca tttgggaaag
      181 tctgtacgag gcttcagaca acgcgctcag tcttgctagc ggtgcattct gggccaatgg
      241 tattgaaaca gggaaccaac agatccccaa gacccaggaa tatctggccg aatttttcgc
      301 ggctgtagac agcgcggatg ctcttcttga taaagatgtc atagtctttc gcaatggcac
      361 ctaccaagac atgatcactg gtgacccgat tcctgagccc ggcacgctgg ccttactcgg
      421 tgccgccttg gtgggctggg gcctggcccg gcgcaagccg gctccaaaag tctgacaacg
      481 acctagcaag tgctgcgaac acgctgtcgt gccgggggca ggatgccacg gaaagttcgg
      541 tcttggccca tggagtgatg gggaagtacc atccaagcct tggtttgcta ttgatacaat
      601 agcttctcac gcagactgta taagggctat cggcatattt gactataaac tgctggtcta
      661 actgaggttg cctgcaacat tggtcaattg ctgttccata aagcgcgagg cattttccag
      721 gtcgttgacc gtgctgcctg actccaacgc caagacaaag cgacgccacg gcaaatcggg
      781 ttcgcctacc tcaaaggcgt aaaagtggcc gaaagcgtta tgtttatgcc acacggcctg
      841 cgcttgcagc gacgaccgct cggcctgtcc gagttcaatt tcgacttggc cccaggtgaa
      901 cagtggcaag ggatcatcac acataacctg aaaaccgttt tccgaaacat ccaccagctt
      961 gagttgccag cttttctgct ctgcctgcgg gtttagtcgc agcatgcagg ggcatttgac
     1021 cgaaaagcgt tggaagctac gcaacaaatg tgcccgggta gtcgcttcag gcaggctagg
     1081 caacaccgcg cggtaggctg gcaaattgta aattgcatgc aaaaggtgta tttttcggtc
     1141 ttcaagttcg gcaaacccct gagtccgctg gttaaaaaaa tagtccagca aagcgggtgt
     1201 catgaccggg tcgttggtgg tgtgataacg gcgagcgggc aactcgatat tgggcaaggt
     1261 caattcattg aaataatgat gcaggttgcg ccgcagcggc ttatgggtat agatcggcct
     1321 gaacttctgc gcagcatggt gcagatcgag cgtagctccg acggccggtg cgccattggc
     1381 aaagtcatag ttgtcgacgg tgttttgcgg cagccggtca aacagcagca gcgattcata
     1441 cagttcgatg cggttcggat tgaccgcaat caccagatgc cgggtgtcga agaagccagt
     1501 gcagtaatcg tgcatgaact tcatcagcgg aaagagcact gagcccccgg tcttgcgaaa
     1561 cttcgggtgt actgccaagg cggatacttc tgcaatcatg ccgccttgct cgcgtactga
     1621 atgcaggtca aagatgctct gtagcggaaa acccatcaca ctttcgcgga tcaatgacag
     1681 cgtgccgacg acctcgccgt cgaacgtggc acacaaggtt gtggtggtag gtaaggcatg
     1741 gtagagcgtg acacgcaacc cggaagggtg aggtttcata aagccgctgg ccacataggc
     1801 atcgtgcagc aagccaaagc aggcttccag ttcgtcgcgg gtctcggcaa ttttcaggtg
     1861 caggcgcggg tctggcgcgg ggtcgcagtc gacaaatgag cggtatgctg caaaccgcac
     1921 agtgcgcggt agcagagaaa aagcctggcg cagcgcccgg tgccaactgg cccgaagttt
     1981 ggaagggttc atgctgagtc agtcaggcga gctgacacct aggcactgca aagcagtagc
     2041 ggcttggatc ggtttcgtgc tgggcatttt ggagattgat acagacatag gagggtcatt
     2101 gggcattttg cgcgtctttg aaatatctgg ggctttgtca gcaaagaggg gcaagctgtg
     2161 gcatgagcag catgtcaagc acccgataca gggggagtcc agagattcga aaggcgcgaa
     2221 tcccgcacgt gtctataaag gtcactttca cacgtttcat gttttcctcc tcatgtattc
     2281 ctccagttta cagcggtcag agttaagtca attgacggcc tggccgtaca gccgagccat
     2341 tcgctcaaac gctggtcgcg atgctcaaaa gccggacatt gttataaatt taatagctgt
     2401 cagtgcaata ctggtaatgg ctagagtgca aaagtattca aaaatgattg caacaatatt
     2461 catcaagtaa atgcgaccga cagtgaagat gcatcgatgg cgaggtcaaa ccagccgttg
     2521 atatcttcat gcgatgcaga cttaatcgaa gcgatcatac ttggcaaaaa tgtgtccaaa
     2581 gcgcttgttg aggatggcta tcgaaaaacg cggaaatctg actgcaaagc tatttgtggg
     2641 gacgggacat gcgcgcctgg ggttttggaa cgaacccgaa gggccagttc atcaccccat
     2701 ggcgcgcggc gctggagatt tgggtggcgg aattggattt ttcctgagga cttgtcccgc
     2761 attgaactgg gtgcacctgg actagcccaa cagtccaaga atgagtggaa actgactttg
     2821 ccgtaaaagc tggagcaggc acgacaccgg ccatcgaagt aacaagttca agccgatgtg
     2881 tgtgccccga gcccgagagg gcaaggttgc cggtgcaagt acctcaccaa cacatcctgc
     2941 gctgtgcagc cgctgtgacc tcggtaccgg aattggcaaa caccagcgtt catcacggta
     3001 gcgcatacaa cttgctcatc acacgccaag caaacctgga tgccgcattt gaggctcatc
     3061 cgactcgctt taaaggcaaa tcctcacaat cacctgtctt gcccagcgcc gcctggacaa
     3121 atccacctcg ccagccagga gaaaaacaac cctcaacgct caaccttgcg cagcaaattt
     3181 atgcacaatc gtgttgccaa gccattggcc agttccggtc aattactgtg catcaaattg
     3241 cacgcagcac gaaaaggaga gtttcatgac ggctctgatg aatgcaatca acaacgaaaa
     3301 cgtttcgctg gtgggacaac tgataaggca aggcattgat gtcaacatgt tggatgcaaa
     3361 cggggatgcc cccttggtca tggctgccta caaaggcaac ccggacattg ttcggatgtt
     3421 gctgcaggcg ggggcagatg ttaaggcagt tgacccggcc atgaaagcaa cggccctgca
     3481 tgcggctgct tatgcaggca acgccgatgc tgccaaacta ttggtgcagt accacattga
     3541 catagacaag caggggccaa tgaacggcta caccgctttg cacgatgcca tttggcaagg
     3601 acatctggag accgcaaagg tgctgattga ttctggggcg aacctgacgc ttcaatcact
     3661 gcatggcgaa acaccgctcg cctttgcccg gcaaaaacac cgcagcgcca ttgcggcact
     3721 gattgagcgc aagttagcct ccaagtagac agcctttcca acaggcaaat gggtcaaggg
     3781 gagacctgat cggcctcgcc ttgttctact ccgcagctat cttgaccacc tgcttgccgc
     3841 gtacccgtcc atcaagttgc tgatgcaggg catgcgttac tgccttggca tcaaaagcca
     3901 ctgccgtcat tggcagtggc aagatggcac ccgtggttaa cagagccagc acctcgtcgc
     3961 ccattttggc caaatccaat tgcgcggctt ggtcacccga aagccaggca ccaccgaggg
     4021 tgatgaaatg caggctcggt gccatgcgaa agacgttgat cttggaaaca tcaggcaggt
     4081 tgatgaggca tgccattgac cctgcataag ccagcaaacc caagtcgcgc tgcccgcttg
     4141 cgccaccgag cgaatcgagc accgcatcca cgccacgtcc accagtcaac tgcaacacgg
     4201 actctcttac atcctcgttc cgataatcaa tcacatggtc agcacccaac tcgctgacgt
     4261 acttgcggtt gacatcggaa caagtcgcaa tcacggttgc gcccagatgc ttggccatct
     4321 gcacggcgaa gccgccaaca ccgcccgcgg cagcttcaat caacatagtg ggcattgtct
     4381 gacgcgactt caagtagagc tttcgcgtca gtgcctgcca ggcagtccag gcagcacacg
     4441 gcagcgtcgc agcagtcagc aggctgacag tttgcggcac ctcgcaaact gctcgctccg
     4501 caatcagtgc aaactctgcc agtgttcctg gtttggacag ggggaaatgc gccaggaccc
     4561 ggtctccaat ggcgaaactt gtcacacctt tgccaagcgc atcaacccaa cccgcgccat
     4621 cgacccctgg aatatgcgga taagtccaag cggaattgcc gcccgtagcc agtttggcat
     4681 ccaccggatt gagggccgcc gcacagaggc gaacccgtat ttcacccaaa gcaggttcgg
     4741 gcaaaggcat cgctcccatt ctgagcgtaa aggcgtgctg tgggtgatca agcaatagtg
     4801 cgttcatgca atggagtgta ccgaggcgag cgcaagagcg gttcggtcgt ccctcgaaat
     4861 ttccgatccg gccaggctca caccacctcg gacaatgccg caaggtctcc caaagaagaa
     4921 tgggtctatg atcgttcggt gatgaaaatc gctcagtgag ctgcggggca tcgaacaccc
     4981 actaaagtgg ggaaccgccc caaaagacgt gtaatttgcc cgctgtaatt aacccgacag
     5041 gagaatgaac atgacactca cccaacaacg catcatgatc gttggtggtt ccaatggtat
     5101 gggactggct gctgcacagc gccttgctcg tgccaaagca gatgttgcga tagtcggtcg
     5161 ctcacaggcg aaacttgaca ccgctctggc gacaatcgac ggcaacgcca gcgcctttgt
     5221 agctgatttc actgacactg cttcgctggc ggcactcgtt gagcaggtcg gcagcattga
     5281 ccatttggtg cttgtggcat ccagcaatgc ggcatttggg ccgttttcca ccacctctgc
     5341 cgaagccttg cgccgcatgc tggaaaacaa acttttgggg tactggcaga cccttcaggc
     5401 aacgttgcca catttgcgtc gcgatggctc agttgtaatg gtcactggcg cagcctcgcg
     5461 gacggcagtt tctggaatgg ctgggcttgc cgctgttaac ggcgccatca cgcagttggc
     5521 gcagacgctt gccaaggaat tggcaccgct gcgggtcaat gtcgtgtcgc cggggctggt
     5581 tgatacgccg gtctatgatc aaatgccagc ggaagcaaaa gcccggatgt tcgccgatgc
     5641 tgccaagtcg cttcctgttg gtcgcgtcgg aacggtcgat gatattgcta tggcaattga
     5701 gttcctgatt gcgaatagcc acaccacagg tgcattgatc gatgttgacg gcggtgctcg
     5761 catggctggc tagca
//
LOCUS       DAOSLL010000271         5769 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12552_length_5769_cov_4.445397, whole genome shotgun sequence.
ACCESSION   DAOSLL010000271 DAOSLL010000000
VERSION     DAOSLL010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12552_length_5769_cov_4.445397"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..461)
                     /locus_tag="PLB25_12210"
     CDS             complement(<1..461)
                     /locus_tag="PLB25_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOE42376.1"
                     /translation="MPRTPSASPIQVFFALLPDSLALDWAGPAEALRMANLALQAQGR
                     PARFGLRFIGPEAETTSSVGLKLAGLEPLPDTLPEPAWVVLVGQPGATLAVQAAAPQA
                     LLHWLRGLRLQPQRLELVTVCAGAVLAAHAGLLCHRHVTTHHHHLAELQAV"
     gene            complement(490..1056)
                     /locus_tag="PLB25_12215"
     CDS             complement(490..1056)
                     /locus_tag="PLB25_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOE42377.1"
                     /translation="MKTCLILIDAQESFRQRPYFKDADLPAYLAAQNALIDGCAAQGI
                     PIVRIFHVDGPKTIGNPFALESGFVQPLAQLHPFDAAATFYKCRHSALVGTGLDVWLT
                     QQGISRLIISGIRTEQCSETTTRHASDLGWTVDYCLDATLTWDMQHQDGSVLLAADIK
                     ARTATVLHDRFATVCSVPQALARAVAAT"
     gene            1295..1609
                     /locus_tag="PLB25_12220"
     CDS             1295..1609
                     /locus_tag="PLB25_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOE42378.1"
                     /translation="MILELADFSIHPGQNAAFEEAIKRGLAAVIAQAKGFEGFKVNRC
                     IENPQRYVLQIFWTTLENHTVDFRESPAFPAWRAIVGPFFAAPPVVQHFDLVAKSESL
                     KG"
     gene            1615..2310
                     /locus_tag="PLB25_12225"
     CDS             1615..2310
                     /locus_tag="PLB25_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HOE42379.1"
                     /translation="MDYCFVPAPTVSVPVVGQSAEFPVHRIYCVARNFEDHAREMGQT
                     SREPPFFFMKPADAVLVASAGQTATLPYPSLTHNLHHEIELVVAIGVGGKDIPVEQAR
                     SHIFGYAVGLDMTRRDLQNEMKKQGRPWCIGKGFDHSAPIGPITPAQHLPDIDQANIS
                     LQVNGSVRQQSQLSQMIWNVPETIAQLSAAWELQPGDLIYTGTPAGVAAVVPGDSMTG
                     SISGLTPISLKVG"
     gene            2413..2958
                     /locus_tag="PLB25_12230"
     CDS             2413..2958
                     /locus_tag="PLB25_12230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009515264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOE42380.1"
                     /translation="MQRPPVKHCRNCGTAVVCRVPDDGDTRLRGVCPACHTVHYENPL
                     NVVGTIPYLDQRVLLCKRNIEPRLGKWTLPAGFMELCETVAEGAARETVEEAGAQFEM
                     GEFFSLVNIPRVGQVHMFYRARLLSEQFDPGHETLEARLFLEDEIPWDEIAFKTVGET
                     LKRYFADRRAGQFTIHVFDIN"
     gene            3118..4563
                     /locus_tag="PLB25_12235"
     CDS             3118..4563
                     /locus_tag="PLB25_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HOE42381.1"
                     /translation="MLPCYLLLDLETTGGNALQDRITEIAAVRFEAGMEVARWSTLVN
                     PGMRISFFIEQLTGISNGMVADAPAFDEVGGQLLKLLDGAVLVAHNVRFDHSFLLAEF
                     ARMGVTLHIKTLCTVRLSRLLYPQVKGHGLDAIMQRHGISSQNRHRAMGDVEVVQSWL
                     AQVQQQFGDDKLRQQAQTLLQASAVLPPQLDTRIADIPDAAGVYIFYGEGTLPLYVGK
                     SVKLRSRIMSHFQAASREPREMRIAQEIRRIEWLETAGELGALLLEARLVKQHQPLHN
                     RQLRRASALSAWRLDANPYSRPLLTLVRGSELNPAQFGNLYGPYRSKNQAHNHLRELA
                     DTHGLCLQALGLEAGKGRCFAHQIGHCKGVCCGEEAPERHHLRLQMALVADKLRVWPF
                     AGRVGLREYNPASGRTDIHVFDQWCHLATVHCDEELADVLQQRQDALAFDLDTYKLAV
                     KHLLPPGKAGVKVLEFGRTASMAHAGESLPD"
     gene            complement(4635..4811)
                     /locus_tag="PLB25_12240"
     CDS             complement(4635..4811)
                     /locus_tag="PLB25_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42382.1"
                     /translation="MRAWTFQVNRQAAWDRFYRRSSSQPAAPPHDDDVVDVQARDVNV
                     VTDVEPKRITPPGR"
     gene            complement(4907..5239)
                     /locus_tag="PLB25_12245"
     CDS             complement(4907..5239)
                     /locus_tag="PLB25_12245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42383.1"
                     /translation="MTQLLTGLLGLVVRVGLFLAGLVFFASLLAAALLVLAVWLLRAL
                     WAKLTGQPVNPWTFQVNRQAAWDRFYRRSSSQPAAPPHDDDVVDVQARDVNVVTDVEP
                     KRITPPGR"
     gene            complement(5399..>5769)
                     /locus_tag="PLB25_12250"
     CDS             complement(5399..>5769)
                     /locus_tag="PLB25_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HOE42384.1"
                     /translation="SVIFGMQSFGEGLDLPGQLCETVFIAKLPFASPSDPVEEARAEW
                     LKREGRDPFSELVVPATGIRLLQWTGRAIRTETDQARVVCYDKRLLSMSFGRRMLQGL
                     PPYAVSRRVRGQTLAVPGTV"
BASE COUNT         1206 a         1746 c         1710 g         1107 t
ORIGIN      
        1 tccaccgcct gcaactcagc caggtgatga tggtgggtgg tcacatggcg gtggcacagc
       61 agacccgcgt gggcggccag cacggccccg gcgcacactg tcaccaattc cagcctttgg
      121 ggttgcagcc gcaggccgcg caaccagtgc agcaacgctt gtggtgcggc ggcttgcacg
      181 gccagggtgg cacccggctg gcccaccagc accacccagg ccggctccgg cagcgtgtca
      241 ggcaggggct ccaggccagc gagtttgagc ccgaccgagc ttgttgtttc agcctcgggg
      301 ccaataaagc gcaacccaaa gcgcgctggc cgaccctgcg cctgcaaggc caggttggcc
      361 atgcgcaagg cttcggcagg accggcccag tccagcgcca ggctgtcagg tagcagggca
      421 aaaaaaacct gaatcgggct tgctgacggc gtgcggggca tggtgagttc gaacaacttc
      481 ggggcagact caggtggctg ctaccgcgcg cgccagcgcc tggggcacgc tgcagacggt
      541 ggcaaagcgg tcatgcaaca cggtggcagt gcgcgccttg atgtcggcgg ccagcaaaac
      601 gctgccatcc tggtgctgca tgtcccacgt caatgtggcg tccaggcagt agtccaccgt
      661 ccagcccaaa tcagaggcgt ggcgcgtggt ggtttcggag cattgttcgg tgcggatgcc
      721 actgatgatc aggcgcgaga tgccttgctg cgtcagccac acgtccagac ctgtgcctac
      781 cagtgcactg tggcggcatt tgtaaaaggt ggcggctgca tcaaacggat gcaattgcgc
      841 caggggttgc acaaagcccg actcaagagc gaacgggttg ccaatggttt tggggccatc
      901 cacatgaaaa atccgcacaa tcggtatgcc ctgggctgca cagccgtcaa tcaaggcgtt
      961 ttgtgcagca agataagctg gcagatcggc atctttgaaa tacggtcgct gacgaaacga
     1021 ctcttgggca tcaatcaaaa taagacatgt tttcatgaaa tcaactccgg ttgtgaatgc
     1081 cctcatggtg catgaccatc aagcattgag ccatactgcg gcagaccagt atcgatcaaa
     1141 tcaggacatt tttgttgggc acctgattgg cgcccgagcg gtcaaatctg gaaaaccctc
     1201 aaaatatgta acaaaattgg ccaccagcct atgtcaataa agcgctgata gctatctgat
     1261 ttgcagtcct tcaacccacc caccgagagc tttcatgatc ctcgaacttg ccgatttttc
     1321 catccatcct ggccaaaatg ccgcgtttga agaagccatc aaaaggggcc ttgctgccgt
     1381 aattgcacaa gccaagggct ttgaaggctt caaggtcaac cgctgcatcg agaacccgca
     1441 gcgctatgtg ctgcaaattt tctggaccac gcttgaaaac cacacggtgg actttcgcga
     1501 atcgcccgcg tttcccgcct ggcgcgccat cgtcgggccg tttttcgccg caccgccagt
     1561 ggtgcagcac tttgacctgg tggccaaatc cgagtcgctg aaaggctaag cacgatggac
     1621 tattgttttg tgccggcgcc taccgtgtcg gtgcccgttg tggggcagtc ggccgaattt
     1681 ccggtgcacc gtatttactg tgtcgcccgc aactttgaag accacgcccg ggagatgggc
     1741 cagaccagtc gcgaaccgcc gtttttcttc atgaaaccgg cggacgcggt gctggtggcc
     1801 agcgcggggc agaccgccac cctgccctac cccagcctga cccacaacct gcaccatgaa
     1861 atcgaactgg tggtggccat cggtgtcggg ggcaaggaca tcccggtgga acaagcccgc
     1921 tcgcatattt tcggctacgc cgtgggcctg gacatgaccc ggcgcgactt gcaaaacgag
     1981 atgaaaaagc agggtcgccc gtggtgcatc ggcaaaggtt ttgaccattc agcaccgatt
     2041 ggtccgatca cgccagcgca gcacctgcca gacattgatc aggccaacat cagcttgcaa
     2101 gtcaatggca gcgtgcgcca gcaaagccag ctcagccaga tgatctggaa tgtgccagaa
     2161 accattgcac aactttctgc cgcctgggaa ctgcagcccg gtgacctgat ttacaccggc
     2221 acgcctgcag gcgttgccgc cgtggtaccg ggtgacagca tgaccggcag catcagcggc
     2281 ctgaccccga tcagccttaa agtcggctaa acaacacagt gctgcgccag aaaacatcag
     2341 caaagttggc acacctgttg attttgtggc acgccaggca tggctttacg ggtttcaaat
     2401 tacactgcaa gcatgcaacg tccccctgtc aaacactgca gaaactgtgg caccgccgtg
     2461 gtttgtcggg tgcccgatga cggtgacacc cggctgcgcg gtgtctgccc ggcctgtcac
     2521 acggtgcact acgaaaaccc gctcaacgtg gtgggcacca ttccttacct ggaccagcgg
     2581 gtgctgctgt gcaagcgcaa catcgagccg cgcctgggca aatggacgtt gccggccggt
     2641 ttcatggagc tgtgtgaaac cgtcgcagaa ggcgcagcgc gcgaaaccgt cgaggaagcc
     2701 ggtgcgcagt ttgagatggg cgaatttttc tcgctggtga atattcccag ggtcggccag
     2761 gtgcacatgt tttaccgtgc ccggctgctg agcgagcaat ttgaccccgg ccatgagacc
     2821 ctggaagccc gcctgtttct tgaagacgaa atcccctggg atgagattgc cttcaaaacc
     2881 gtgggtgaaa ccctgaaacg ctactttgcc gaccgccgcg ccggacagtt cacgattcat
     2941 gtctttgata tcaactgact tgcaagccac cctgtcactg tggttttatc gcgctggcca
     3001 tttttttgtc accagcgtgc ttgacgaccc ggcctggctg gccctgctgt tttgttgatg
     3061 ttttgtgcct ccagcccttg tggcgtatgc gctaatagct attatttttg attgtatatg
     3121 ctgccttgtt acctgctgct ggacctcgag accaccggtg gcaatgcact gcaggaccgc
     3181 atcaccgaaa tagcggcggt gcggtttgag gctggcatgg aagtggcgcg ctggagcaca
     3241 ctggttaacc cgggcatgcg catttccttc tttatcgagc agctcaccgg catcagcaat
     3301 ggcatggtgg ccgatgcacc ggcgtttgat gaagtgggcg gccagttgct gaagttgctc
     3361 gacggagccg tgctggtggc gcataacgtg cgttttgacc acagtttttt gttggctgaa
     3421 tttgcccgca tgggcgtgac gctgcatatc aagacgctgt gcacggtgcg cctgtcgcgc
     3481 ctgctgtatc cgcaggtgaa gggccatggc cttgatgcca tcatgcagcg ccacggcatt
     3541 agcagccaga accgacaccg cgccatgggc gatgtcgagg tggtgcaaag ctggctggca
     3601 caggtgcagc agcagtttgg cgacgacaag ctccgccagc aggcccagac cctgctgcaa
     3661 gccagcgcgg tactgccgcc gcagctcgac acccgtattg ccgacattcc tgatgccgct
     3721 ggcgtttaca ttttttatgg cgagggcacg ctaccgctgt atgtcggcaa gagcgtcaaa
     3781 ctgcgcagcc ggatcatgtc gcactttcag gccgccagcc gcgagccgcg cgaaatgcgc
     3841 attgcccagg aaattcgccg cattgagtgg cttgagaccg ccggcgagct gggcgcgctg
     3901 ctgctcgaag cgcggctggt caagcagcac cagcccctgc ataaccggca actgcgccgc
     3961 gccagtgcct tgagcgcctg gcggctcgat gccaacccgt acagtcgccc gctgctgacg
     4021 ctggtgcgcg gcagtgagtt gaaccctgcg caatttggca acctgtatgg cccgtaccgc
     4081 tccaaaaatc aggcgcataa ccatctgcgc gagcttgccg acacgcatgg gctgtgcctg
     4141 caggcgctgg gactggaggc tggcaagggc cgttgttttg cccaccagat tggccattgc
     4201 aaaggtgtct gctgcggtga agaagcaccc gaacgacatc acctgcgcct gcaaatggcg
     4261 ttggtggccg acaagctgcg ggtctggccg tttgccgggc gggtcggttt gcgcgagtac
     4321 aacccggcta gcgggcgcac cgacatccat gtgtttgacc agtggtgcca tctggccacg
     4381 gtgcactgcg atgaggaact cgccgacgtg ttgcaacagc gccaggacgc gctggcgttt
     4441 gatctcgaca cctacaaact ggcggtcaag cacttgttgc cgccgggcaa ggccggggtg
     4501 aaggtgcttg agtttggccg cacggcgtca atggcgcatg ccggggaaag tctgccagat
     4561 tagccgacca tttgaatcaa aaatgccacc agcccttgtt gcatatgctc tgatagctat
     4621 aactaaaata gcattcagcg ccccggtggg gtgatccgct tgggctccac gtcggtcacc
     4681 acgttgacat cgcgcgcctg cacatcgacc acatcgtcat cgtgcggggg tgctgcaggc
     4741 tggctggacg aacggcggta aaaccggtcc caggcggcct gccggttgac ctgaaaggtc
     4801 caagcgcgca agccggggga agtctgccag attagccgac catttgaatc aaaaatgcca
     4861 ccagcccttg ttgcatatgc tctgatagct ataactaaaa tagcattcag cgccccggtg
     4921 gggtgatccg cttgggctcc acgtcggtca ccacgttgac atcgcgcgcc tgcacatcga
     4981 ccacatcgtc atcgtgcggg ggtgctgcag gctggctgga cgaacggcgg taaaaccggt
     5041 cccaggcggc ctgccggttg acctgaaagg tccaagggtt aaccggctga ccggtcaatt
     5101 tggcccacag cgcgcgcaac agccagaccg ccagcaccag cagcgccgca gccagcagac
     5161 tggcaaaaaa caccaaaccg gccaaaaaca ggccgacacg cacgaccaaa cccaataatc
     5221 ctgtcaacaa ctgagtcaca aacgcactcc ttcagatcgc agcaaatggt gcttgcatgc
     5281 cagactgcaa ggcctgtcat tgcagttcca agtcattgct ctcaaggcac ccaggcgcag
     5341 ccattgcaca cgcaaggcag agcaaagcaa ccagggcacc gatgattgag acatggcatc
     5401 agactgtgcc gggcaccgcc agcgtctgcc cacgcacccg gcgcgacacc gcatagggtg
     5461 gcaggccttg cagcatgcgc cgaccaaacg acatgctcag caagcgcttg tcataacaaa
     5521 ccaccctggc ctgatcggtc tcggtccgaa tggcgcggcc ggtccactgc agcagccgga
     5581 tgccggtggc gggcaccacc agttctgaaa acggatcgcg cccctcgcgt ttgagccatt
     5641 cggcgcgcgc ttcttcgacc gggtctgacg gcgaggcaaa cggcagcttg gcgatgaaca
     5701 cggtttcgca caactggccg ggcaagtcca gcccctcgcc aaacgactgc atgccaaaaa
     5761 tcaccgacg
//
LOCUS       DAOSLL010000272         5760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12579_length_5760_cov_4.237862, whole genome shotgun sequence.
ACCESSION   DAOSLL010000272 DAOSLL010000000
VERSION     DAOSLL010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12579_length_5760_cov_4.237862"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            103..912
                     /locus_tag="PLB25_12255"
     CDS             103..912
                     /locus_tag="PLB25_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE42385.1"
                     /translation="MEPKNIVLNVNGIEVIYNHVILVLKGVSLQVPEGGIVAILGGNG
                     AGKTTTLRAISNLLAGERGEVTKGSIELRGERIENLSPADLVERGVVQVMEGRHCFAH
                     LTIEENLLTGGYTRKSKAEIAANLEKVYNYFPRLKTRRTSQAAYTSGGEQQMCAIGRA
                     LMANPSMVLLDEPSMGLAPQIVEEVFEIVKDLNAKEKVTFLLAEQNTNMALKYADYGY
                     IMESGRVVMDGAAADLRTNEDVKEFYLGVGGGERKSFKDVKSYKRRKRWLA"
     gene            926..2200
                     /locus_tag="PLB25_12260"
     CDS             926..2200
                     /locus_tag="PLB25_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE42386.1"
                     /translation="MPHSFFDALEKRVPAQREEALLAELPRQIAHAKLSSAAFADLLK
                     DVDPAAVTSRAALARLPVIRKSELQERQQTSRDAQAGAVASGDVFGGFSALEYGAFMP
                     RVFASPGPIYEPEGTGKDYWRMARAMYAAGFRPGELIHNCFSYHFVPAGSMMETGAHA
                     MGCTVFPGGTGQTEQQVQAMRDLKPAGYIGTPSFLKIIVEKATELGLTLPSVKKAMFG
                     GEAYPPSLRDWFTARGIDGYQCYATADLGLIAYETQAREGLVLDEGVIVEIVRPGTGD
                     PVAEGEVGELVVTTLSGGYPLVRFGTGDLSAVLPGACPTGRTNTRIKGWMGRADQTTK
                     IRGMFVHPKQVDDIVKRYPEIVRARLVVTGEMANDTMTLKVETGANPDGLAARIGESI
                     RDVTKLRGTVELLQPGSLPNDGKVIEDARSYK"
     gene            2301..3272
                     /locus_tag="PLB25_12265"
     CDS             2301..3272
                     /locus_tag="PLB25_12265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013544074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOE42387.1"
                     /translation="MKKLLVVAAAVSAMFAQAQTFPSKPISIVVPFSAGGPTDRVARD
                     LAEALRKPLGGVSILIDNVAGAGSSIGTNKVAKAAPDGYTLLVAHIGMSTMPALYRNL
                     PFKVESDFEYLGMINDVPMTLIGRPTLPAKNYKELVTWIGQNKGKINLGNAGLGAASH
                     LCGLMFQTALQTDMTTVPYKGTAPAMTDLMGGQIDLMCDQTTNTTQQIEGKKVIAYAV
                     TTAKPLTTPVLKDLPTLQSAGLKDFEVTIWHGLYAPKGTPAEVTAKINAALKVALKDP
                     EFVKKQEALGAVVVSDKRIEPAEHKKFVAAEIAKWGPVIKAAGVYAD"
     gene            complement(3294..3623)
                     /locus_tag="PLB25_12270"
     CDS             complement(3294..3623)
                     /locus_tag="PLB25_12270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HOE42388.1"
                     /translation="MYKFKSRAAGDLIMLDPNGQQILTIIGKFDGPADRKGILLPEQM
                     PAAIEALESAVAQDEARRAQLIKEAQDAGEAPPAFEGISLRLRAVPFIAMIRRCHKED
                     KEIVWGV"
     gene            3646..3975
                     /locus_tag="PLB25_12275"
     CDS             3646..3975
                     /locus_tag="PLB25_12275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HOE42389.1"
                     /translation="MADLHILREHTLGFAAARKVAYRWAEQVEGEFDMQCTYEEGKAG
                     DRVSFERKGVHGTLHVTKDRFELDAKLGFLLGAFKDRIESEIVKNLDSLLAPTTAAAK
                     KAGAAKK"
     gene            complement(4035..4289)
                     /locus_tag="PLB25_12280"
     CDS             complement(4035..4289)
                     /locus_tag="PLB25_12280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HOE42390.1"
                     /translation="MATLKAKFEAAVANSKSLTERPDNSTLLKIYALYKQATVGDNTE
                     KKPGFTDMVGRAKWEAWNGFQGVDAKDAMQQYIDLIESLS"
     gene            4378..>5760
                     /locus_tag="PLB25_12285"
     CDS             4378..>5760
                     /locus_tag="PLB25_12285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007862562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HOE42391.1"
                     /translation="MVTRVTAKKATPKARSTAQVVQKSVADAVVGTLGLHGERMSKVD
                     TAWLRMDTDSNLMMIVGVWVIKPGIRYDDLAERLEERLLRYPRFVQCAVEDAAGATWI
                     RDRNFSMSNHLVVEKLPRTAAKDQQKALQDRLAKLCMEPLDRTHPLWRFHLVEHYKGG
                     SALMVRIHHCIADGIALISVTNSLMDGGKVPPMRPTRLHPHGGLEGAEDWFEETLLKP
                     LTSLAVKALGAAGEGAARSFDMMADPQKGVEKGLHGSVDAAKLAFQMVGDLAALALMP
                     DDSHTSLKGLPGKTKRVAWCPPLPLAEVKAVGKALNCSINDVLLSCVAGAIGEYLKAN
                     GELTAGKEIRAMVPVNLRPLDQAYKLGNQFGLAPVVLPVGMDNPVERLYEVRRRMGGL
                     KGSMQPLLAFGLLAVAGLLIKPAQDAMLSLFSKKTTAVMTNVPGPREKLKICGSTIEE
                     SLFWVPQSGTV"
BASE COUNT         1205 a         1567 c         1836 g         1152 t
ORIGIN      
        1 tccccaaccc ctcccgccaa gcgggagggg cagcagctct cacgagctgc cagatgaaag
       61 cgtcgcgcgg cggagctttc atctggtcaa caaggctgag ttatggaacc caaaaacatt
      121 gttttgaacg tgaatggcat cgaggtcatt tacaaccacg tcatcctggt gctcaagggc
      181 gtttcgctgc aggttccgga aggcggcatc gtcgccattc tgggcggcaa tggcgccggc
      241 aagacgacga cgctgcgtgc catttccaac ctgctggcag gcgaacgcgg cgaggtgacc
      301 aaggggtcca tcgagctgcg tggcgaacgc atcgagaacc tgtcgcctgc cgatctggtg
      361 gagcgcggtg tggtgcaggt gatggaaggg aggcattgtt ttgcccacct gacgattgaa
      421 gaaaacctgc tgaccggggg ctacacccgc aagagcaaag ccgaaatcgc agcgaatctg
      481 gagaaggttt acaactattt tccgcgcctc aagacccggc gtaccagcca ggcggcctat
      541 acctcgggtg gtgagcagca gatgtgcgcc attggccgtg cgctgatggc caaccccagc
      601 atggtgctgc tggacgagcc ctcgatgggc ctggccccgc aaatcgtgga agaggtgttt
      661 gagatcgtga aggacctcaa tgccaaggaa aaagtcacct ttttgctggc cgaacagaac
      721 accaacatgg ccctcaagta cgctgactat ggctacatca tggagtccgg ccgggtggtg
      781 atggatggag ccgccgctga cctgcgcacc aatgaggacg tcaaggagtt ctatctgggc
      841 gtgggcggtg gtgagcgcaa gagtttcaag gacgtcaaga gctacaagcg tcgcaagcgc
      901 tggttggcgt aagggagtac acaccatgcc ccattcattt tttgacgccc tggaaaaacg
      961 cgtgcccgct cagcgggagg aagcgttgct ggctgagttg ccgcgccaga ttgcccatgc
     1021 caagctgagt tctgcggctt ttgccgactt gctcaaggac gtggatcctg cggctgtcac
     1081 cagccgtgca gcgttggcgc gactgccagt gatccggaaa tccgagttgc aggaacgcca
     1141 gcagacttcg cgtgacgcac aggctggcgc cgttgccagt ggcgatgtat tcgggggatt
     1201 cagcgcgctg gagtatggcg cgttcatgcc gcgggtattt gccagtccgg gcccgattta
     1261 cgaacccgaa ggcacaggca aggactactg gcgcatggcg cgcgcgatgt atgccgcagg
     1321 gttccggccg ggcgagctga ttcacaactg cttcagctac cactttgttc ccgctggctc
     1381 aatgatggaa actggagccc atgccatggg ttgcaccgtg ttccccggcg gcactggcca
     1441 aaccgaacag caggtgcagg ccatgcggga cctgaaaccc gcaggctaca ttggcacgcc
     1501 cagtttcctg aaaatcattg tggaaaaggc taccgagctc gggttgacat tgcccagcgt
     1561 caagaaggcg atgttcggcg gcgaagccta tccgcccagc ctgcgggact ggtttactgc
     1621 gcgggggatc gacggctacc agtgctacgc cacggcggat ctggggctga ttgcgtatga
     1681 aacacaagcg cgcgaaggcc tggtgctgga cgaaggtgtg attgtggaga ttgtgcggcc
     1741 gggtacgggg gatcctgtgg ctgagggcga ggtgggagaa ctggtggtga ccacgctgag
     1801 tggcggttat cccctggttc gttttggcac gggggatctg tctgccgtgt tgcccggagc
     1861 ctgtcccacc ggacgcacca atacacgcat caagggctgg atggggcgcg ctgaccagac
     1921 caccaagatt cggggcatgt ttgtgcaccc caagcaggtg gacgacatcg tcaaacgcta
     1981 tcccgagatc gtgcgtgcgc gtctggtcgt taccggggag atggccaacg acaccatgac
     2041 cctcaaggtg gagaccggag ccaacccgga tggcttggcg gcccgcatcg gtgagtccat
     2101 acgggacgtc accaaactgc gtggaactgt ggagttgctg cagcccggca gcctgcccaa
     2161 tgacggcaag gtcatagaag acgccagaag ctacaagtaa gctgcgcccg gggtgcttgc
     2221 tgtggactag gtgtttcccg gggaccgcga gtgtggtggc agcgctagac tatgttggtt
     2281 attgaaccgg agttctacaa atgaaaaaac tactcgttgt cgctgcggcc gtgtccgcca
     2341 tgtttgccca ggcgcagaca tttccttcca agcccatttc cattgtggtg cctttctctg
     2401 cgggcggtcc cacggaccgt gtggcacgcg atctggccga agcactgcgc aagccgctgg
     2461 gtggggtgag cattctcatc gacaacgtcg cgggtgccgg cagctccatc gggacgaaca
     2521 aggtggccaa agccgcaccg gacggttaca ccctgctggt ggcacacatt ggcatgtcga
     2581 ccatgccagc gctgtaccgc aatctgccat tcaaggtgga gagtgatttt gagtacttgg
     2641 ggatgatcaa cgatgttccg atgaccctga ttggccgccc cacattgccg gcaaaaaatt
     2701 acaaggagct ggtgacctgg attggccaga acaagggcaa gatcaatctc ggtaacgcgg
     2761 gcttgggcgc tgcctcccac ctgtgcggtt tgatgtttca gacggccttg cagacggaca
     2821 tgacgacggt cccgtacaag ggaacggcgc ccgcgatgac cgatctgatg ggtggtcaga
     2881 ttgacctgat gtgtgaccag accaccaata cgacacagca gattgaaggc aaaaaagtca
     2941 ttgcctatgc ggtgacaacg gccaagccat tgaccacgcc ggtcttgaaa gatttgccca
     3001 cgctgcagag cgctggactc aaagactttg aagtaacgat ctggcatggg ttgtatgcgc
     3061 ccaagggaac gcccgccgag gtgactgcca agatcaacgc cgcactcaag gtcgcgctca
     3121 aggatccgga gttcgtcaag aagcaggagg ctctgggagc tgttgtagtg tccgataaac
     3181 ggattgagcc tgcagagcac aagaagtttg tggctgctga aatcgccaaa tggggcccgg
     3241 tgatcaaggc tgctggcgtt tacgcggact gatggctgtt gcgcgctctg gggttacacg
     3301 ccccagacga tttccttgtc ttccttgtgg cagcgccgga tcattgcaat aaagggtacg
     3361 gcacgcaggc gtaatgaaat gccctcgaac gcagggggcg cttcacccgc gtcctgcgct
     3421 tccttgatca gctgcgcgcg ccgggcctca tcctgcgcga ccgctgactc cagcgcttcg
     3481 atggctgcag gcatctgttc cggcagcagg attcccttgc ggtcggcagg cccgtcaaat
     3541 ttgccaataa tggtaagtat ttgctgcccg ttagggtcta gcatgatgag gtcacctgcg
     3601 gcgcgggatt tgaatttgta catggctatt tgaaggagaa aaactttggc tgatcttcac
     3661 atactgcggg agcacacact gggattcgct gcggcgcgca aagtagcgta ccggtgggcc
     3721 gagcaagtcg agggtgagtt cgatatgcag tgcacctatg aggaagggaa ggccggagac
     3781 agggtttcct tcgagcgcaa gggagtgcat ggcacgctgc atgtcaccaa agaccggttt
     3841 gagctcgatg ccaaactggg ctttttgctg ggcgctttca aagaccgcat agagtccgag
     3901 atcgtcaaga atctggactc cttgctggcg ccaaccacgg cggcagcgaa gaaagctggc
     3961 gcagcaaaaa agtaagccaa atgctactaa tacagtagca ctccccgcaa tgtttgcggg
     4021 ggctaaagcc tgatttagct taaagattcg atcagatcga tgtattgctg catcgcatcc
     4081 ttggcatcca cgccctggaa gccattccag gcttcccact tcgcgcgtcc caccatgtcg
     4141 gtaaagccag gctttttctc ggtgttgtcg ccaacagtgg cctgcttgta cagtgcgtaa
     4201 atcttgagca gggtgctgtt gtcggggcgc tcggtcaagg atttggaatt ggcaacagcg
     4261 gcttcaaatt tggctttcag ggtggccatg gaattctcca gtaggtttaa ggattcgccc
     4321 cgtatcttac ggtggctttt gactgcagaa tagagttcct acctttctgg agcgtatatg
     4381 gtgacccgag tgactgccaa aaaggcgacc cccaaagcac gcagcacggc acaggtggtg
     4441 caaaagagtg tggcggatgc agtagtgggt accctgggtc tccatggcga gcgcatgagc
     4501 aaggtggata ccgcctggtt gcggatggac acggactcca atctgatgat gattgtgggt
     4561 gtctgggtga tcaagcccgg tatccggtac gacgatctgg ccgaacgcct ggaagagcgc
     4621 ctgttgcgct acccccgatt tgtgcaatgt gcggtggagg acgcggccgg tgcgacctgg
     4681 atacgggatc ggaacttcag catgtccaac catctggtgg tcgaaaagtt gcccagaacg
     4741 gcagccaaag accagcagaa agccctgcag gaccggttgg ccaagctctg catggagccg
     4801 ctggatcgca cccacccgct ctggcgtttc cacctggtgg agcactacaa agggggttcg
     4861 gcgctgatgg tgcgcattca ccattgcatc gccgatggca tcgcactgat ttcggtaact
     4921 aactcgctga tggatggtgg caaggtgcct cccatgcgac ccacgcgcct gcacccgcat
     4981 ggcgggctgg agggcgcgga agactggttc gaggagaccc tgctcaaacc cctgaccagc
     5041 cttgcggtca aggcgctggg tgccgccgga gaaggtgccg cgcggtcttt tgacatgatg
     5101 gcagaccccc agaaaggcgt tgaaaaaggc ctgcacggtt cggtggatgc ggccaagctg
     5161 gctttccaga tggtgggtga tctggcagcg ctggcgctca tgccggatga ctcgcatacc
     5221 agcctgaaag gactgcctgg aaagaccaaa cgcgttgcct ggtgtccgcc attgccgctg
     5281 gctgaagtca aggcggtggg caaggccttg aactgctcca tcaatgatgt gctgctgagt
     5341 tgcgtggctg gtgcgattgg cgaatacctc aaggccaatg gtgagttgac cgcgggcaag
     5401 gaaattcggg ccatggtccc cgtcaacctg cgaccgctgg atcaggccta taaattgggc
     5461 aaccagttcg ggctggcacc ggtggtgctg cctgtcggca tggacaaccc ggttgagcgt
     5521 ttgtatgaag tgcgccggcg catgggcggg ctcaagggca gcatgcagcc gctgctggct
     5581 ttcgggctgt tggccgtggc tgggttgctg atcaagccgg cgcaagatgc catgctcagc
     5641 ctgttctcca aaaagaccac ggcggtcatg accaatgtgc cgggtccacg cgaaaaactc
     5701 aaaatatgcg gctccacgat cgaggaatcc ctgttctggg tgccacaaag cggcaccgtg
//
LOCUS       DAOSLL010000273         5721 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12687_length_5721_cov_3.648959, whole genome shotgun sequence.
ACCESSION   DAOSLL010000273 DAOSLL010000000
VERSION     DAOSLL010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5721
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12687_length_5721_cov_3.648959"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="PLB25_12290"
     CDS             <1..604
                     /locus_tag="PLB25_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOE42392.1"
                     /translation="GTQAFMRFDYPGTLAADIQARALDVARRFLCAVGFDHGLFNMEF
                     FYDEPTNRLTVIEFNPRMAAQFSDLYLRVRGIDLHQLALALAHGQDPAGVPRSTPTAG
                     AAASFVYRSLSERAVVRMPDAAQQEALTQAFAQALLFCFAKTPSQIARDFKWLGSYRY
                     GILHLAASDAQQLKADCEHASALLGWPAPYADVTAQQGIG"
     gene            651..1256
                     /locus_tag="PLB25_12295"
     CDS             651..1256
                     /locus_tag="PLB25_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing (seleno)protein"
                     /protein_id="HOE42393.1"
                     /translation="MYPLSKLKRLAGATLIAASLAGCGAITAQNPSGHYKPVNAVADD
                     PEARLLLKGADVTAYFTQGKFVQGSAQYRSSYEGVDFRFASAQAKSLFDQSPAQYLPQ
                     YGGFCANGLVYGIPWGGDANTWKMVNGKLYIFGGQGSLDAFELDEKQNLALADKYWNE
                     EVKGSNSFMQRSKRLLLRVPHYKSGEELAKLVEAAKAKAKP"
     gene            1362..2252
                     /locus_tag="PLB25_12300"
     CDS             1362..2252
                     /locus_tag="PLB25_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOE42394.1"
                     /translation="MKRRQALSALGLGAGVVLSGCTMGPQGGDADADTPAQKPTARHY
                     PVAWVLSSGGPRGFVHVGVLKALYQLGHRPDLVVGSSVGALVGCLAAAGVGMARIEAL
                     ALETSMASLFRLNFFGELWLNGAGLAKLVNEAVADRRLQQFDMAFAAVAMRQDAQQLI
                     AFNWGNAGLAVQAACAVEGRLAPVRIAEADYLDADRLSPLPVRIARHCGATRVLAIDA
                     SAHEDQAPPTASAYRATDLRKRELTQADAVHADLVLHPEFGYYTGVSREYRERTIAAG
                     YQQTLLQAQALRQLHATKSA"
     gene            2693..3289
                     /locus_tag="PLB25_12305"
     CDS             2693..3289
                     /locus_tag="PLB25_12305"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOE42395.1"
                     /translation="MDIYDIQRAQAVFQTVFDAYPAAVTLAYVVLFTLLTALCLPGAA
                     VLMLLAGASFGLVWGSLLATLASTAGATLTMLGARHLLRRRVERRYGVLLQAFNQSLQ
                     REGALYLLSLRLLPVIPFVPVNLVSGLTRLNGATFFWVSALGMLPGTAVYVYAGVYLA
                     QLQSLTQLLSARTAVTLMALGVVPLAAALVLRRRRRSS"
     gene            4131..4364
                     /locus_tag="PLB25_12310"
     CDS             4131..4364
                     /locus_tag="PLB25_12310"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HOE42396.1"
                     /translation="MSDPMQRRIFMFMPLFITAVCYNFPSGLTLYWTVQNCFTIFQQW
                     VTNRSKDPVPVQSEKKTLAAPRVAPVKVKSKKR"
     gene            4438..5193
                     /locus_tag="PLB25_12315"
     CDS             4438..5193
                     /locus_tag="PLB25_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE42397.1"
                     /translation="MSGHSKWATTKRHKAAVDAKRGKIFSSLSKEITLAARASGGDTS
                     TNARLRTLIIKAKAANMPNDNIDRAVKKGTGEIPGVVYEEVVYEGYAAGGVGVVVTVT
                     TDNKNRSTSEVRSVFTKHGGNLGSPGALTNFSFHKKGRFIISKEVADEEKLMEVALEA
                     GAEDIKDNGDTFEVLSDMKDYDTISDALNKAGIAAQESALAWIPTSETPIADLDGAKK
                     ILKMIDSLEALDDVNDVYANFDISDELLAQLDA"
     gene            5206..>5721
                     /locus_tag="PLB25_12320"
     CDS             5206..>5721
                     /locus_tag="PLB25_12320"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE42398.1"
                     /translation="MNDAAQTPDFSAGEVGLVRFEDFHSNNPLVFDSGASIPQFTLRY
                     ETYGRLNKTRDNAILVCHALSGDHHCAGVHTFEDRKPGWWNNIIGPGKPIDTNRFFVV
                     CSNCIGGCQGSTGPSSVDPLTGQAYHMNFPEITIRDMVRAQGALIFEHLGIEKLACVV
                     GGSMGGMQALQW"
BASE COUNT         1155 a         1713 c         1723 g         1130 t
ORIGIN      
        1 cggcacccag gcgttcatgc gctttgacta tcccggcaca ctggccgcag acatccaggc
       61 tcgcgccctg gacgtggccc ggcgctttct gtgcgccgtc ggttttgacc acggcttgtt
      121 caacatggag tttttttatg atgaaccgac caatcggctc accgtgattg agttcaaccc
      181 gcgcatggcg gcgcagttct ctgacctgta cctgcgggtg cgaggcattg atctgcacca
      241 actcgcgcta gcgctggcgc atggacaaga cccggccggg gtgccacgca gcacacccac
      301 ggcgggggcg gctgccagtt ttgtttaccg cagtttgtct gaaagggcgg tggtgcgcat
      361 gcccgatgcc gcccagcaag aggccttgac ccaggccttt gcgcaggcgc tgctgttttg
      421 ctttgccaaa acccccagcc agatcgcccg cgacttcaaa tggctgggca gctaccgcta
      481 cggcatcttg cacctggcgg cgtctgacgc gcagcagctc aaagccgact gcgaacatgc
      541 cagtgccctg cttggctggc cagcccccta tgccgatgtc acggcgcagc agggcattgg
      601 ctgatctggc atattcgtgc cccaattcgt caattaccgg agaccacacc atgtacccct
      661 tgtcgaagct gaagcgcctt gccggcgcta ccctgattgc cgcctcgctg gcgggctgtg
      721 gggccatcac cgcacaaaac ccgtcgggcc actacaaacc ggtgaatgct gtggccgatg
      781 accctgaggc ccgcctgttg ctcaagggtg ccgatgtcac cgcctatttc acccagggta
      841 agtttgtaca gggcagtgcg cagtatcgca gcagctacga gggcgttgac ttcaggttcg
      901 cttcagcaca agccaagtcg ctgtttgacc agtcacctgc ccaatacctg ccgcaatacg
      961 gcggcttttg tgccaacggc ctggtttacg gcatcccatg gggcggcgat gccaatactt
     1021 ggaagatggt caacggcaag ctgtacatct ttggcggcca ggggtcgctg gacgcgtttg
     1081 aactcgatga aaagcaaaac ctggcgctgg ccgacaaata ctggaacgaa gaggtcaaag
     1141 gcagcaacag tttcatgcaa cgcagcaagc gcctgctgct gcgggtgccg cactacaaga
     1201 gtggcgaaga gctggccaag ttggtcgaag cagccaaagc caaagccaaa ccctgaacgc
     1261 aagacccgca cgcgttgcaa ttgatttcag acaaaaatgc actccaggcc atatacagca
     1321 agcgcaagca gctattctaa tagtagctac ttggggcagc aatgaaacgc cgtcaggcgc
     1381 tcagtgcact tggcttaggt gctggtgtcg tcctgtcggg ctgcacgatg gggccgcagg
     1441 gcggcgatgc cgatgccgac acacccgcgc aaaagccaac cgccaggcac tacccggtgg
     1501 cttgggtact cagttcaggt gggccgcgcg gttttgtcca cgttggtgtg ctcaaagcct
     1561 tgtatcaact cgggcaccgg cccgatctgg tggtgggcag ttcggtcggc gcgctggtcg
     1621 gctgtctggc tgccgctggc gttggcatgg cgcgtatcga ggcgctggcg ctggaaacct
     1681 caatggcgag cctgttccgg ctcaactttt ttggcgagct gtggctcaat ggtgctggcc
     1741 tggccaagtt ggtgaacgag gccgtggctg accgccgatt gcaacagttt gacatggcgt
     1801 ttgcggctgt ggcgatgcgc caggacgcgc aacaactgat cgccttcaac tggggcaatg
     1861 ccggcctggc ggtgcaggcg gcttgtgccg tggaagggcg gctggcaccg gtgcgcatcg
     1921 ccgaggctga ttatctggat gccgatcgcc tcagcccgct gccggtgcga atcgccaggc
     1981 attgtggtgc cacacgggta ctggccatcg acgcgtccgc tcacgaggac caggcaccgc
     2041 ctacggccag cgcttaccgt gcaacggacc ttcgcaaacg tgaattgacg caggccgatg
     2101 cggtgcatgc tgacctggtg ctgcacccgg aatttggcta ttacaccgga gttagccgtg
     2161 agtaccgcga acgcaccatt gctgcgggct accaacagac gctgctgcaa gcccaggcgc
     2221 tgcgtcagtt gcacgcgacc aagtctgcat gatccaagat tcatccgcga gcagcatgtt
     2281 ttcatcttca agtcctcatg agcatttgtt tatctagctt cgtggaggct ccaccctggg
     2341 tgacagcgct gcgcacccaa ttgagcgatt ggcaacccgt ccggggtcgt tgcccttcct
     2401 gtttgcgcca ggatgacatg cgctcaggtg ccagggtcgt cgtcgccact cgcagcttgc
     2461 tgccgcatgc aaggcgcaca gtcatgccac aaggctggcc tgctcaaagc cgtccaaacg
     2521 tgcgctggca ggtttggatt tgcgtctttc gcactacccg gcgccgccgc cagcggttca
     2581 atcgggtgca taacgctgtg acccattagg tgaactccat caaagccaaa aaagcaaata
     2641 ctcataggga cttacagatg ataacagacg gtcaaccgag gaaccttggc tgatggacat
     2701 ttacgacatc cagcgcgcgc aggcggtctt tcagaccgtt tttgatgctt acccggcagc
     2761 ggtgacactg gcctatgtgg tgctgttcac cttgcttact gcactgtgcc tgcccggtgc
     2821 agcggtgttg atgctgctcg ccggtgccag ttttggactg gtctggggca gcctgctggc
     2881 cacactggcg tccactgccg gggccacgct gaccatgctg ggggctcgcc atctgctgcg
     2941 ccgccgggtc gagcggcgtt acggcgtgct gctgcaggcc ttcaaccaga gcctgcaacg
     3001 cgaaggtgcg ctgtacctgc tgtcgctgcg gctgctgcca gtcatcccgt ttgtgccggt
     3061 gaacctggtc agtggtttga cgcgtttgaa cggggccacg tttttctggg tcagcgcgct
     3121 gggcatgctg cccggtacgg ctgtgtatgt gtatgccggg gtttacctgg cgcagttgca
     3181 gtcgttgacg caactgctgt cagcccgcac tgccgtcacg ctgatggcgc tgggtgttgt
     3241 gccactggct gcggccctcg tgctgcgcag acgcagacgc agcagttgac aggtgttttg
     3301 gcagcgacaa accgggcaag acttggcgat ttcccgttct atcgagccag tgcctgatct
     3361 ccaggtcgtg cggcattgct tcagcacaac ggtgttgagc gcaggcactg tcatgctgct
     3421 ggggctcgtc tcggtttttt ttgccgggca aatggggctg cagacctcgc ctcgctgcct
     3481 tttgtgtcgg tcagggctga tcaggtacca actcggtgac gggtggagga tgtgtctggc
     3541 tagaaagtct catcgacccg cttttggata gcagggattg atgggcaaaa agtattgcgc
     3601 atggctgatg ccaggtgctg cagcaagctg cctcatggtc tggctgaaag ggcaacagtt
     3661 gccgacttgc agttgagcgg gcgagggcga ccacagcgcc tttcaattta tgaaaaattg
     3721 acctctagcc cctgtggaat atgcgctgga agctagtgcg ccagcgaaag ccggtagatc
     3781 gtagttgatg aaagttcaat acagggaaga aatagcgaat cactctggcc ccgagtcatg
     3841 cgttgtacat cgcgaggtgt acatcgaagc gttgacaggg gagaccggca ggccagccat
     3901 tgagccgcga aattgcgcaa atacaggatg caggcggagt tagctttcac ggcaatgcaa
     3961 cacagaccaa ggcgttacac gcaagccttg gcctgatcct gcgcggtcaa agaccctgag
     4021 catgctggga agcgatctac tcggaagctg ggagatctca tcggtgtccg agccacgagg
     4081 cacgggcggg acaggcaagg cccaaagcca caaccctgtc gcccacgccg atgagcgacc
     4141 cgatgcagcg gcgcatcttc atgttcatgc cgttgttcat cacggcggtg tgttataatt
     4201 ttccctccgg cctcacgctt tattggacgg tgcaaaactg cttcaccatt ttccagcagt
     4261 gggtgaccaa tcgctcgaaa gaccccgtgc ccgtgcaaag cgaaaaaaag acgctcgccg
     4321 ctccgcgcgt ggcgccggtg aaggtcaaat ccaaaaagcg ctgacacgcg agcggaaaat
     4381 cctttttcac ggcatctctt tttgctaact taaactcaac tccaactcac agtactcatg
     4441 tcaggacata gcaaatgggc aaccaccaag cgccacaagg cggccgttga cgccaagcgc
     4501 ggaaaaatct tcagctcgct gagcaaggaa atcaccctgg ccgcgcgcgc cagcggcggg
     4561 gacacctcca ccaacgcgcg cctgcgcacc ttgatcatca aggccaaggc cgccaacatg
     4621 cccaacgaca acatcgaccg cgccgtcaaa aaaggcacgg gcgaaatccc gggcgtcgtt
     4681 tacgaggaag tggtgtacga gggttacgcc gcgggcggcg tcggcgtcgt ggtgacggtc
     4741 accaccgaca acaagaaccg ctcgacctcc gaggtgcgca gcgtcttcac caagcacggc
     4801 ggcaacctcg gctctcccgg cgcgctgaca aatttttcct tccacaaaaa aggccggttc
     4861 atcatctcca aggaggtggc cgacgaggaa aaactcatgg aagtggcgct cgaagccggc
     4921 gccgaggaca tcaaggacaa cggcgacacc tttgaggtct tgtccgacat gaaggactac
     4981 gacacgattt ccgacgcgct gaacaaggcg ggcatcgccg cgcaggaatc cgcgctggcg
     5041 tggattccca ccagcgagac cccgatcgcc gacctggatg gcgccaagaa aatcctgaag
     5101 atgatcgact cgctcgaagc cctcgacgac gtcaacgacg tgtacgccaa tttcgacatt
     5161 tccgacgaac tgctcgcaca gctggatgcc taaaaaattc cattcgtgaa cgacgccgca
     5221 cagacgcccg atttttccgc aggagaagtc gggcttgttc gattcgagga tttccactcg
     5281 aataatccgc tggtgttcga cagcggggcg agcatcccgc agtttacctt gcgctacgag
     5341 acttacgggc gcctcaacaa aacgcgcgac aacgcgatcc tcgtgtgcca cgccctaagc
     5401 ggcgaccatc actgcgcggg cgtgcacacc tttgaagacc gcaagcccgg ctggtggaac
     5461 aacatcatcg gcccgggcaa gccgatcgac acgaaccgct tttttgtcgt gtgctccaac
     5521 tgcatcggtg gctgccaagg ctccacgggg ccttcgagcg tcgacccgct gacaggccag
     5581 gcctaccaca tgaatttccc cgagatcacc atccgcgaca tggtgcgtgc gcaaggggcg
     5641 ctcatcttcg agcatctagg cattgaaaaa ctcgcgtgcg tggtcggcgg gtcgatgggc
     5701 gggatgcagg cgctgcagtg g
//
LOCUS       DAOSLL010000274         5720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12688_length_5720_cov_3.704678, whole genome shotgun sequence.
ACCESSION   DAOSLL010000274 DAOSLL010000000
VERSION     DAOSLL010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5720
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12688_length_5720_cov_3.704678"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /gene="rplN"
                     /locus_tag="PLB25_12325"
     CDS             <1..246
                     /gene="rplN"
                     /locus_tag="PLB25_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HOE42399.1"
                     /translation="SIKEAAPRGRVKKGDVYSAVVVRTAKGIRRSDGSLVKFDGNAAV
                     LLNAKLEPIGTRIFGPVTRELRTEKFMKIVSLAPEVL"
     gene            256..576
                     /gene="rplX"
                     /locus_tag="PLB25_12330"
     CDS             256..576
                     /gene="rplX"
                     /locus_tag="PLB25_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HOE42400.1"
                     /translation="MNKIRKGDDVIVLAGRDKGKRGKVALRKDDSHLVVEGVNVVKKH
                     AKPNPMKGVAGGIIEKTMPIHQSNVAIFNAATGKADRVGIKLQADGKRVRVYKSSGEE
                     IKVA"
     gene            579..1118
                     /gene="rplE"
                     /locus_tag="PLB25_12335"
     CDS             579..1118
                     /gene="rplE"
                     /locus_tag="PLB25_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HOE42401.1"
                     /translation="MARLQQIYREKLAPELIAKFGYKSPMEVPRLTKITLNMGVSEAV
                     ADKKVMDHAVGDLTKIAGQKPVVTMSKKAIAGFKIRENQAIGCMVTLRGAQMYEFLDR
                     FVTVALPRVRDFRGISGRSFDGRGNYNIGVKEQIIFPEIEYDKVDALRGLNISITTTA
                     KTDDECKALLAGFRFPFKN"
     gene            1127..1432
                     /gene="rpsN"
                     /locus_tag="PLB25_12340"
     CDS             1127..1432
                     /gene="rpsN"
                     /locus_tag="PLB25_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004197948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HOE42402.1"
                     /translation="MAKMALIERELKRDKLVAKYAKRHAELKAISTDAKRTGEERAAA
                     RLGLQMLPRNANPTRQRNRCAITGRPRGTFAHFGLARAKIREMAFAGEIPGIVKASW"
     gene            1451..1846
                     /gene="rpsH"
                     /locus_tag="PLB25_12345"
     CDS             1451..1846
                     /gene="rpsH"
                     /locus_tag="PLB25_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HOE42403.1"
                     /translation="MSMSDPIADLLTRIRNAQMVAKTSVRIPSSKVKVAIAQVLKDEG
                     YIDGFKVNVTDGKQELEIALKYYAGRPVIERLERVSRPGLRVYRGSDAIPQVQNGLGV
                     AIVTTPKGVMTDRKARATGVGGEVLCYVA"
     gene            1873..2406
                     /gene="rplF"
                     /locus_tag="PLB25_12350"
     CDS             1873..2406
                     /gene="rplF"
                     /locus_tag="PLB25_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HOE42404.1"
                     /translation="MSRIGKMPVAIPAGVDVSLGEQQVTVKGSGGMLLVAQSRLVKVG
                     SEGGKLSFTAANDSREADAMVGTMRQLVNNMVLGVTKGFEKKLILIGVGYKAQAQGNR
                     LNLAVGYSHPVNKDMPDGIVVTTPTPTEILIKGADRQRVGQIAAEIRAIRPPEPYKGK
                     GIRYSEEKVAIKETKKK"
     gene            2418..2783
                     /gene="rplR"
                     /locus_tag="PLB25_12355"
     CDS             2418..2783
                     /gene="rplR"
                     /locus_tag="PLB25_12355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HOE42405.1"
                     /translation="MLTKKEQRLRRARQTRIRIANQGVARLTVNRTNLHIYASVISGD
                     GSKVLATASTVEAEVRQSLGGIGRGGNVAAAHIIGKRLAEKAKAVGVEKVAFDRAGFA
                     YHGRVKALADSAREAGLQF"
     gene            2801..3325
                     /gene="rpsE"
                     /locus_tag="PLB25_12360"
     CDS             2801..3325
                     /gene="rpsE"
                     /locus_tag="PLB25_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HOE42406.1"
                     /translation="MAKVQAKMQGKAEGPEDGLREKMIAINRVTKVVKGGRILGFAAL
                     TVVGDGDGTVGMGKGKSKEVPAAVQKAMEEARRNMAKVSLKNGSIHHRVIGQHGAASV
                     MMAPAPKGTGIIAGGPMRAVFEVVGITDIVAKSHGSSNPYNMVRATLDALSHATTPSI
                     VAAKRGKTVEELFA"
     gene            3340..3522
                     /gene="rpmD"
                     /locus_tag="PLB25_12365"
     CDS             3340..3522
                     /gene="rpmD"
                     /locus_tag="PLB25_12365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HOE42407.1"
                     /translation="MTTEKKVTVQLVRSPIGCAASHRATVRGLGLRKVRSTSELVDTP
                     EVRGMINKISYLVKVL"
     gene            3533..3964
                     /gene="rplO"
                     /locus_tag="PLB25_12370"
     CDS             3533..3964
                     /gene="rplO"
                     /locus_tag="PLB25_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HOE42408.1"
                     /translation="MELNSIKPSDGAKHFKRRVGRGIGSGLGKTAGRGHKGQKSRAGG
                     YHKVGFEGGQMPMQRRLPKRGFKSDLLKFNAEVTLLALEELDVTEINLLVLKQRGLVG
                     QLARVVKVIKTGATTKVLKLSGIGASAAAAAAIKAAGGTLA"
     gene            3983..5293
                     /gene="secY"
                     /locus_tag="PLB25_12375"
     CDS             3983..5293
                     /gene="secY"
                     /locus_tag="PLB25_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HOE42409.1"
                     /translation="MATSSVSTSKKDKYGDLRRRLVFLLLALVVYRIGAHIPVPGIDP
                     VQLQQFFKGQQGGILGMFNMFSGGALSRFTIFALGIMPYISASIIMQLMGYVLPAFEQ
                     LKKEGEAGRRKITQYTRYGTLGLALFQSLGIAVALESSAGLVISPGFGFRVTAVVTLT
                     AGTVFLMWLGEQITERGLGNGISILIFGGIAAGLPNAIGGLLELVRTGAMSIVVSLFI
                     VLLVALVTYFVVFVERGQRKILVNYARRQVGNKVYGGQSSHLPLKLNMAGVIPPIFAS
                     SIILLPATVVGWFSAGESMRWLKDIAGTLTPGQPIYVLLYAAAIIFFCFFYTALVFNS
                     RETAENLKKSGAFVPGIRPGDNTAKYIDKILLRLTFVGAIYITLVCLLPEFLILKYNV
                     PFYFGGTSLLIIVVVTMDFMAQVQNYLMSQQYESLLKKANFKAS"
     gene            5303..5521
                     /gene="infA"
                     /locus_tag="PLB25_12380"
     CDS             5303..5521
                     /gene="infA"
                     /locus_tag="PLB25_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018149800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HOE42410.1"
                     /translation="MAKDDVIQMQGEIVENLPNATFRVRLENGHIVLGHISGKMRMHY
                     IRILPGDKVTVELTPYDLSRARIVFRAK"
     gene            5554..5667
                     /gene="rpmJ"
                     /locus_tag="PLB25_12385"
     CDS             5554..5667
                     /gene="rpmJ"
                     /locus_tag="PLB25_12385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HOE42411.1"
                     /translation="MKVSASVKKICRNCKIIRRKGIVRVICTDARHKQRQG"
BASE COUNT         1425 a         1202 c         1632 g         1461 t
ORIGIN      
        1 agcatcaaag aagctgcacc gcgcggacgc gtcaaaaaag gcgatgtcta cagtgcagtt
       61 gttgtgcgca ccgccaaggg cattcgccgc agcgatggct cgctggttaa atttgacggc
      121 aatgcggcag ttttgctcaa tgccaagctc gagccaatcg gcacgcgcat cttcgggcca
      181 gtgacgcgcg agttgcgtac tgaaaagttc atgaagatcg tgtctttggc acctgaagtt
      241 ttgtaaggac gtctcatgaa caaaattcgc aaaggcgacg atgtcattgt gctcgctggg
      301 cgtgacaaag gtaagcgcgg gaaagttgcg cttcgcaagg atgactctca tctcgtggtt
      361 gagggagtca atgtcgttaa aaagcatgcc aagccaaacc cgatgaaggg tgttgcgggt
      421 ggcattatcg aaaagaccat gccgattcac cagtctaacg tcgcaatatt caatgcggcg
      481 actggcaagg ctgatcgggt tggtatcaag ttgcaggctg atggcaagcg cgttcgcgtt
      541 tacaaatcaa gcggcgaaga aatcaaggtg gcatgaacat ggctcgtttg caacaaatct
      601 accgtgaaaa actggcgcca gagttgattg ccaagtttgg ctacaagtcg ccaatggaag
      661 tgccacgctt aacaaagatc actctgaata tgggtgtgag cgaggctgtc gctgacaaga
      721 aggtgatgga tcacgcggtg ggtgacctca ccaagatcgc gggtcaaaaa cctgtagtca
      781 cgatgtcaaa aaaggcgatt gccgggttca agattcgtga aaaccaggcc attggctgta
      841 tggtgacgct tcgtggcgct cagatgtacg agtttttgga tcggtttgta accgtcgctt
      901 tgccgcgcgt acgagatttc cgtggtatct ccggtcgttc ttttgatggg cgtggaaact
      961 acaacatcgg cgtcaaagag cagatcattt ttcctgaaat tgagtatgac aaggtggatg
     1021 ccttgcgtgg tctcaacatc agcatcacaa cgacggccaa gactgatgac gagtgcaaag
     1081 cactactcgc tggtttccgt ttccccttca agaactgagg tgacctgtgg ctaagatggc
     1141 tttaatcgag cgcgaactca aacgagacaa actggttgcc aagtatgcaa aaaggcatgc
     1201 cgagttgaaa gcgatttcaa ccgatgcgaa gcgcactggc gaagagcgtg ctgccgctcg
     1261 tctcggtctg caaatgttgc cacgcaacgc aaacccaacc cgtcagcgta accgttgtgc
     1321 gatcactggt cgccctcgcg gtacctttgc ccacttcggt ttggctcgtg ccaaaattcg
     1381 tgagatggct tttgcgggtg aaattcccgg catcgtcaag gctagctggt aagcggtagg
     1441 agacatacaa atgagcatga gtgatccgat cgccgacctc ttgacgcgca ttcgcaatgc
     1501 gcagatggtg gcgaaaacat ctgttcgtat accttcctcc aaagtgaagg ttgcgattgc
     1561 ccaagttttg aaggatgagg gctacattga tggttttaaa gtgaacgtta ctgacgggaa
     1621 gcaggagctt gaaatagccc tcaagtatta cgcgggtcgt cctgtaattg aacgtctcga
     1681 acgcgttagt cggccaggcc ttcgtgtgta tcggggcagc gatgccattc cgcaagttca
     1741 aaatggtctt ggtgtcgcga tagtcacaac acccaagggt gtgatgactg atcgtaaagc
     1801 gcgcgctact ggtgtcggtg gtgaagtgct ttgctacgtc gcttgatgcg tgacatttag
     1861 gagaaataga acatgtctcg tattggaaaa atgccggttg ctattccggc cggggtagat
     1921 gtctcgctgg gtgagcagca agtgactgtc aagggctcag gtggaatgtt actggtcgcc
     1981 caaagtcgcc ttgtgaaggt tggcagcgaa ggtggcaagc tcagctttac tgctgcaaat
     2041 gattcacgtg aggctgatgc aatggttggg acgatgcgtc agttggtcaa taacatggtc
     2101 cttggtgtta ccaagggctt tgaaaaaaag ttgattctga ttggtgtggg gtacaaggct
     2161 caagctcaag gtaatcggtt aaatctggcc gtaggttatt cccatccggt caataaagat
     2221 atgcctgatg ggattgttgt aacgacaccg acacctactg aaatattgat taagggtgca
     2281 gatcgtcagc gtgtagggca gatagccgca gagattcgag ccattcggcc gccggagccc
     2341 tacaaaggca aaggaattag gtattcggaa gaaaaagtcg ccattaaaga aaccaagaag
     2401 aaataaggaa ctgcatcatg ttgaccaaaa aggagcagcg tcttcgcagg gctcgtcaga
     2461 cgcgtatacg tatcgccaac caaggggtgg ctcgtctgac tgtgaatcga acaaacctgc
     2521 acatttatgc cagcgttatt tctggagatg gcagcaaggt gctcgcaaca gcttccaccg
     2581 tggaggccga agttcgccaa agtctgggtg gaattggccg gggtggtaac gttgccgccg
     2641 ctcatattat tggtaaacgt ttggcggaaa aggcaaaggc ggttggtgtt gagaaagtgg
     2701 cgtttgatcg ggcggggttt gcgtaccatg gccgcgtgaa agcgctggct gattcggcgc
     2761 gtgaagctgg cttgcagttc taagcagatc ggaaaacaga atggctaaag tacaagcgaa
     2821 aatgcagggc aaagctgagg gtcctgagga tgggttgcgc gaaaagatga tcgcgatcaa
     2881 ccgtgtgacc aaggtcgtca agggtggtcg catcctgggc tttgcagcgt tgacagttgt
     2941 tggcgacggt gatggcactg ttggtatggg caagggaaag tccaaagagg ttcctgctgc
     3001 tgtgcaaaag gcgatggaag aagcacgtcg caacatggca aaggtgtcct tgaagaatgg
     3061 atccatccat cacagggtga taggtcagca cggggcagct tcggtcatga tggcaccagc
     3121 gccaaaaggc actggcatca ttgcgggagg tccgatgagg gctgtgtttg aagtcgtggg
     3181 aatcacagac atcgttgcca agagccacgg ctcaagtaac ccttacaaca tggttcgcgc
     3241 aactcttgac gcactttcgc atgcaacaac gccttctatc gtggctgcca agcgcggtaa
     3301 gacggttgaa gaactgtttg cctgatcgga gtactcgaaa tgacaactga gaaaaaggtc
     3361 acggttcaac ttgttcgcag ccctattggc tgtgctgcgt ctcatcgtgc aaccgtccgc
     3421 gggttgggtt tgcgcaaggt gcgtagcacc agcgaactgg ttgatacgcc tgaagtgcgc
     3481 ggcatgatta acaagatcag ttatctggtc aaagtgcttt gaaaggttga tgatggaact
     3541 caatagcatt aagcccagcg acggtgctaa gcactttaaa cgtcgggtcg gtcgtggaat
     3601 tggatcgggt ttggggaaaa ctgctggtcg tggacacaaa ggtcagaagt ctagggctgg
     3661 tgggtaccac aaagtcggct ttgaaggtgg acaaatgccg atgcaacgtc gcttgccaaa
     3721 gcgcggattc aagtcagatt tacttaagtt caacgctgaa gttacgttgc tggccttaga
     3781 agagttggat gtgactgaga ttaatcttct tgttctcaag caacgcggtt tggttgggca
     3841 attggcgagg gtcgtcaaag ttatcaagac aggggccact accaaggttc taaagctgtc
     3901 aggtatcggt gcttctgctg ctgccgcggc tgcgattaaa gctgctggtg gaactttggc
     3961 ctagtttgaa agcgcgctgt cagtggcaac cagttcagtt tcaacctcga aaaaagacaa
     4021 atacggtgat ctacgccgtc gactggtctt tttgttgctc gcacttgtcg tgtatcggat
     4081 aggagcgcat attcccgtac ctggaatcga tccagtccaa ctgcaacagt tttttaaggg
     4141 tcaacaaggg ggcatccttg ggatgttcaa tatgttttct ggtggggcgc tttcgcggtt
     4201 caccattttt gcgctgggga ttatgccgta tatctccgca tcaatcatca tgcagttaat
     4261 gggatatgtg cttccagcgt ttgagcaact taagaaggag ggcgaagcag gacgtcgcaa
     4321 aatcacccag tacacccgct acggcacttt gggcttggcg ctttttcagt cattgggtat
     4381 tgctgttgcg cttgaaagct ctgctggttt ggtcatctct cccgggtttg gcttcagggt
     4441 aaccgcagtc gtaaccttga cggcaggaac agtatttttg atgtggttag gggagcaaat
     4501 taccgagcgg ggtttgggta acggaatttc aatactgatt tttggtggta tcgctgccgg
     4561 tcttccgaat gcaattggtg gattgttgga attggttcgc actggtgcta tgagcattgt
     4621 tgtttcactg ttcattgtcc tgctggtagc tttagtaaca tatttcgttg tgtttgtaga
     4681 acgtggtcaa aggaagatac tggtcaatta cgctagaagg caagttggca acaaggttta
     4741 tggtggacag tcaagccact tgccattgaa gttgaatatg gctggcgtaa tcccaccaat
     4801 ttttgcatcg tctatcattt tgctacctgc gactgttgta ggttggttta gtgccgggga
     4861 atcgatgcga tggttgaagg acatcgctgg aactttgact ccaggtcaac caatttacgt
     4921 tttgttgtac gccgcagcca ttattttctt ttgctttttc tatacagctt tggttttcaa
     4981 cagccgagaa acagcagaga acttaaaaaa gagtggtgca tttgttccgg gtatacgacc
     5041 tggtgataat acggctaagt atattgacaa gatattactt cgattgacgt ttgttggtgc
     5101 aatctacatc acacttgttt gtttattgcc tgagtttttg attcttaaat acaacgttcc
     5161 tttttatttt ggtggtacat ctttgctgat tatcgttgtc gtaacgatgg atttcatggc
     5221 gcaggtgcag aactacctga tgtcacagca gtatgagtcg ctgttaaaga aggctaattt
     5281 caaggcttct tgagggaatc atatggctaa agacgatgta attcaaatgc agggagaaat
     5341 tgtagaaaac cttcctaacg cgacctttcg ggtgaggctc gaaaatgggc acatcgtgtt
     5401 ggggcatatt tctgggaaaa tgcgaatgca ttatattcgt atactgcctg gagataaagt
     5461 tactgttgaa cttacgcctt atgatttgag tagagctcga atcgtgttca gggcaaaata
     5521 gcaaaatgtt aatgaattgt ttaggagaat agaatgaaag tttcggcgtc agttaagaaa
     5581 atttgccgta actgcaaaat tattcggcgc aaaggcattg ttcgcgtgat ctgtacagat
     5641 gcgcggcaca agcagcgcca gggttaattg caagagttat agaggataaa aatggcacgt
     5701 atcgctggta tcaatattcc
//
LOCUS       DAOSLL010000275         5717 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12697_length_5717_cov_4.721477, whole genome shotgun sequence.
ACCESSION   DAOSLL010000275 DAOSLL010000000
VERSION     DAOSLL010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5717
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12697_length_5717_cov_4.721477"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1181)
                     /locus_tag="PLB25_12390"
     CDS             complement(<1..1181)
                     /locus_tag="PLB25_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOE42412.1"
                     /translation="MTRPLRYALVSVRELLASAGPFVVLACALLALAYFWLDPTPPKQ
                     VTLATGPAQSAYDEFGKRYAQALAAYGIEVKLVESEGAAHNLELLTRGQVDLGFVQGG
                     SGDPQAAAQQQLVSLGSLFVEPLWLFYRSPKALNGLADFKKMRMNIGTTGSGVPRLMS
                     QLFELNRMKESELILTRLSQTPATVTFLNNGLDALLFASAPESSMVQMLLQTPGVRLM
                     NFAQSEAYSRRFPFLTPVTLPRGVVDLAKDIPPQDMPLVATTTTLLAGPMVHPAVLQL
                     FSQAALSLHGKAGWFSRARDFPNVELTEFVVSDEAQRAMRNGIPLLQRYLPFAYANLI
                     ERMWLALGIILAVLLPLSRIVPPLYQFRIRSRVFRWYGQLRSIEERAVDQTEALPTLL
                     E"
     gene            complement(1194..1640)
                     /locus_tag="PLB25_12395"
     CDS             complement(1194..1640)
                     /locus_tag="PLB25_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HOE42413.1"
                     /translation="MSLKDQITEDMKNAMRARDSERLGAIRLLLAAAKQKEVDERVVL
                     DDVAMVAIVDKLIKQRKDSVTAYLQAQRQDLADKESFEIKVLQAYLPQRLSSEEITAA
                     VTAIVASLAAKGPGDMGKVMSAVKSQLAGKADLALVSQAVKAALVG"
     gene            complement(2031..2243)
                     /gene="rpsU"
                     /locus_tag="PLB25_12400"
     CDS             complement(2031..2243)
                     /gene="rpsU"
                     /locus_tag="PLB25_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HOE42414.1"
                     /translation="MTTIRVKENEPFDVALRRFKRTIEKLGLLTDLRAREFYEKPTAE
                     RKRKKAAAVKRNYKRIRAMQLPKKLY"
     gene            complement(2609..3742)
                     /locus_tag="PLB25_12405"
     CDS             complement(2609..3742)
                     /locus_tag="PLB25_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013207213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE42415.1"
                     /translation="MTRFSWRRALAVFIKEFQQMMRDRLTFAMAVGVPIMQLVMFGYA
                     INTDPKGLPTVVVSSDAGPMSRSLLAAMQNTGYFRVDHVLGSEKEADALIEAGQVQFM
                     VVIGPHFTRQLIRGEHPAILVSADATDPSASVNAIAALNVMAAQALSHDMGGALRSLQ
                     PQRPPFEVRVHRRYNPEGLSRYNIVPGLVGTILTMTMVMLTSLAMTRERERGTLENLL
                     ATPVRPAEVMVGKILPYVVIGYIQLGVILGAAFLLFEVPMEGSMALLVAMIGVFIVAN
                     LGVGFTFSTIAKNQLQAMQMTFFFFLPSILLSGFMFPFRAMPVWAQWVGEVLPLTHFL
                     RIVRGILLKGNTAAQLLPELWPMLLFLLLAGLLALKRYQQTLD"
     gene            complement(3739..4677)
                     /locus_tag="PLB25_12410"
     CDS             complement(3739..4677)
                     /locus_tag="PLB25_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019450127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE42416.1"
                     /translation="MTDLAIDVRSLSKRYDGRAVVDGISLQVGSGRICGFLGPNGSGK
                     TTTIRMLCGLLTPDGGSGTCLGLDIFKEATAIKRQVGYMTQKFGLYDDLSIRENLDFV
                     ARLFELPQRRQAVDLALEQLGLTARQKQLAGALSGGWKQRLALAACLIHQPRLLLLDE
                     PTAGVDPKARRDFWDQIHALAAQGITVLVSTHYMDEAERCHDLVYIAYGKILARGSER
                     EIIEAARLQVWSITGIGLADVIAPLKASPGVISVAAFGNAVHVAGQDEALLGQALERI
                     RQDYPVQISPAQANLEDVFITLIATAQDNFASGGSP"
     gene            complement(4674..>5717)
                     /locus_tag="PLB25_12415"
     CDS             complement(4674..>5717)
                     /locus_tag="PLB25_12415"
                     /inference="COORDINATES: protein motif:HMM:NF024829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HOE42417.1"
                     /translation="VKPLLSPRQSRGITCFIYAMPTEKTDHRSRALLIAAVSMLVACS
                     DKPAPAWSGYAEGDYVYVAAPLAGRLDAVTVKPGQNIVKGAPLFVLDSEQENAASNEA
                     AARLAVAQAQSANLDKGKRSEELAVMQAQLAQAQAAAALARQELQRQQQLITQGFVAK
                     ARVDDAMTVLAQAQARETELQAALQVAGLPARSDERRASQASTNAAAEVLHQSQWRAG
                     QKKQVAPADALVAEVFFQVGEFIPAGQAVLSLLPPQNIKARFYVSEADLASLQPGQGV
                     TLHCDGCTSPLVAHISRIATQPEFTPPVIYSNAQRSKLVFLVEARPEPADAARLKPGQ
                     PLDVRRNDAAKAP"
BASE COUNT         1179 a         1682 c         1681 g         1175 t
ORIGIN      
        1 tcctccagca gtgtcggcaa cgcttcggtc tggtcaacgg cacgttcctc gatgctgcgc
       61 agttgaccgt accagcgaaa cacccttgac ctgatgcgga actggtagag cggcggcacg
      121 atgcggctta agggcagcag caccgccaaa atgatgccta atgccagcca catgcgctca
      181 atcaggttgg cataggcaaa gggcaagtag cgttgtagca ggggaatgcc attgcgcatc
      241 gcgcgctgcg cctcgtctga tacgacaaat tcggtgagct cgacgtttgg aaaatcgcgt
      301 gcccggctga accagccggc ctttccgtgc aggctcagag cagcttgtga aaacagttgc
      361 agcaccgcag gatgaaccat ggggccagcc agcagggtgg tggtggtggc gaccagcggc
      421 atgtcctgtg gcggaatatc tttggccaga tcgaccacac cgcggggcag ggtaacgggt
      481 gtcaaaaatg gaaaacgcct tgaataggcc tcgctttggg caaagttcat cagccgcacg
      541 cctggtgtct gcaacagcat ctgaaccatg gaagattcgg gggctgaggc aaacagcagg
      601 gcatccaggc cgttgttgag gaaagtcacg gtggcaggtg tttgcgacaa acgggtcagt
      661 atcagttcgg actctttcat gcggttgagt tcaaacaatt ggctcatcag ccgcggcaca
      721 ccactgccgg tcgtaccgat gttcatgcgc atttttttaa aatcagcaag cccgttcaag
      781 gctttgggac tgcggtaaaa cagccacaga ggttcaacaa acagactccc aagcgagacc
      841 agttgctgtt gcgccgctgc ctgagggtcg ccgctgccac cttgtacaaa gccaagatca
      901 acctggccac gggtcagcaa ttcgaggttg tgcgcggccc cttccgattc gaccagcttg
      961 acttcgatgc cgtaggctgc aagtgcctgg gcgtagcgtt tgccaaactc gtcataagcg
     1021 ctttgggctg gcccggtggc cagcgttacc tgcttgggcg gggtcgggtc aagccaaaaa
     1081 taggccagtg ccagcagggc acaggccaac accacaaacg gcccagccga ggccagcagc
     1141 tcgcgtaccg agaccagcgc atagcgcagc ggcctggtca tggcaatccg gagttaacca
     1201 accagcgcgg ccttgactgc ctgtgacacc agcgccaggt cggctttgcc agctaattgg
     1261 gacttgaccg cgctcatgac tttgcccatg tcacctggcc cttttgctgc caggctggca
     1321 acgattgcgg tgacggcagc ggtgatttct tcgctgctca ggcgttgcgg caagtaggct
     1381 tgcagcacct tgatctcgaa gctctccttg tcggccaggt cctggcgctg ggcctgcagg
     1441 taagccgtca ccgagtcctt gcgttgcttg atgagcttgt caacaatggc caccatggcc
     1501 acgtcgtcaa gcaccacgcg ctcatctact tctttttgct tggcggcagc gagcagcagg
     1561 cggatcgcgc ccagacgttc gctgtcccgg gcgcgcatgg cgtttttcat gtcttcggtg
     1621 atctggtctt tgagtgacat tggatttgtt cctgaagaag ttggcaaagc gctcgggtgg
     1681 aaatcagggt aagtttgttc acttggtatc tctgccattc ttgccatcgc gggcttgatg
     1741 gcaaatcaac gacgtaatcc gtttctgcat cgttcgtgtc taggcaccct gccgcaggca
     1801 ttgcaaacgc aaggcaaagc gagataacaa cgatctggat ggattagaaa tggaattagc
     1861 ggaaaaacag ggcagtcctg ggtgaagatg gccgaattct tgacataaaa aaacccgcct
     1921 gagcgtcccc ggcgagcttg tttcaggcct tgcagccctg tcagtctaat caacgagaga
     1981 caaatcctgc tcgcttaaaa accgggcatc ttgtcccgca gtgtttcaga tcagtacagc
     2041 tttttgggca actgcatggc gcgaatgcgc ttgtagttgc gcttgacggc agcggctttc
     2101 ttgcgtttgc gttcggcggt gggtttttcg tagaactcac gggctcgcaa atcggtcaaa
     2161 agaccaagtt tttcaatggt gcgcttgaag cggcgcagcg ctacgtcaaa aggttcgttt
     2221 tctttaacac ggatggttgt cattgaatca aaatatccat aaggtgcaga cttcaaaatc
     2281 tggaagatcg ggccaaacat tgatcttgcg aaattccccg ctcaaaagtt gatcaggcag
     2341 cggagttttg ccagcaatgc ctgcgattat agcgagcgct tcctgtcaat ctcgctgttt
     2401 tcatggattt gtcaatccgt tgctggttgc acgcaacacc aggccgcctg gctgttgaaa
     2461 tgatgcatcg agcggtgaga accgaaccca gcgaggccag cttgtgccag atttgccgat
     2521 ccatggccca gcattcggtc aaaaagccgg tgccaaatgg gccgcagcgg tcggttcgca
     2581 gccgatgccc gatagggttg acagtcacct aatccagcgt ctgttggtaa cgcttcagag
     2641 ccagcagacc cgccagcagc aaaaacagca acattggcca cagttcgggc agcaattgcg
     2701 ccgccgtgtt gcctttgagt aagatgccgc gcacgatgcg caaaaagtgc gtcagcggca
     2761 ggacctcacc gacccactgc gcccacaccg gcatggcccg aaacggaaac atgaagcccg
     2821 acagcaaaat tgatggcaaa aagaaaaaga aggtcatctg catcgcctgt agctggtttt
     2881 tggcgatggt cgaaaaagtg aacccgactc ccagattggc cacgatgaag acaccgatca
     2941 tcgccaccag cagcgccatg ctgccctcca tcggtacctc aaacagtaaa aaagccgcgc
     3001 ccagaatcac acccaactgg atgtagccga tcaccacgta gggcaggatt tttccgacca
     3061 tcacctcggc cgggcgcacc ggtgtggcca gcaggttttc caaggtgccg cgctcgcgtt
     3121 cgcgcgtcat cgccagtgag gtcagcatca ccatggtcat ggtcagaatg gtacccacca
     3181 gaccgggcac gatgttgtag cgcgacagcc cttcagggtt gtagcggcgg tgtacccgca
     3241 cttcgaatgg cggcctttgc ggttgcagcg aacgcagcgc cccgcccata tcgtggctga
     3301 gcgcctgtgc tgccatgacg ttgagtgcag caatcgcgtt gaccgaggcg cttgggtcgg
     3361 tggcatcggc tgacaccaaa atggctgggt gctcgccacg gattaactgc cgcgtgaagt
     3421 gggggccaat caccaccatg aactgcacct gaccggcctc gatcaatgcg tcagcctctt
     3481 tttcgctgcc cagcacatga tcaacccgga aatagccggt gttttgcatg gccgccagca
     3541 ggctgcgcga cattggaccg gcatcgctgc tgaccaccac ggtgggcaaa cccttggggt
     3601 cggtgttgat ggcataacca aacatcacca attgcatgat gggcacgcca accgccatgg
     3661 caaatgtcaa ccggtcgcgc atcatttgct ggaattcctt gataaagacc gccagcgcac
     3721 gccgccatga aaaacgggtc atgggctacc cccgctggcg aaattgtctt gcgcggtggc
     3781 aatcagggtg atgaacacgt cttccagatt ggcctgggcc ggtgagattt gcaccggata
     3841 gtcctgccgg attcgttcca gcgcctgccc caacagtgcc tcgtcctgcc cggcaacatg
     3901 cacggcgttg ccaaaagccg ccacactgat cacacccggc gaggccttga ggggtgcgat
     3961 gacatcggca agaccgatgc cggtgatcga ccagacctga agccgtgcgg cctcgatgat
     4021 ctcgcgctcg ctgccccggg ccagaatctt gccgtaggcg atgtacacca gatcatggca
     4081 gcgctctgcc tcgtccatgt agtgggttga aaccagcacc gtgatgcctt gtgctgccag
     4141 cgcatgaatc tggtcccaaa aatcacgccg cgctttcggg tccacccccg cagtgggctc
     4201 atcaagcaac aacaggcgcg gctggtgaat caggcaggcg gccagtgcca gccgctgttt
     4261 ccagccaccg gacagggcac cggcgagctg tttttgccgt gccgtcaacc ccaactgttc
     4321 cagcgccagg tccactgcct ggcggcgctg cggcagctca aacaggcgcg cgacaaaatc
     4381 caggttttcg cggatcgaca agtcgtcata caggccaaat ttttgtgtca tgtagccgac
     4441 ctggcgcttg atggctgttg cctctttgaa gatgtcgagc cccaggcagg tgccgctgcc
     4501 accgtcgggc gtgagcagcc cgcatagcat gcggatggtg gtggtcttgc cgctgccgtt
     4561 tgggcccaga aagccgcaaa tccgcccgct gccgacctgc aagctgatgc catccactac
     4621 ggcgcggccg tcgtagcgct tgctcaagga tcgaacatca atggccaggt cggtcatggg
     4681 gctttggctg catcattgcg ccgcacgtcc aggggctggc ctggtttaag ccgcgctgcg
     4741 tcggcgggct cgggccgggc ttcgaccaaa aacaccagct tgctgcgctg cgcgttggaa
     4801 taaatcaccg gcggcgtgaa ttctggctgg gttgcaatgc gtgaaatgtg tgccaccagc
     4861 gggctggtgc agccgtcgca atgcagcgtc acgccttgcc ccggttgcag gctggccaga
     4921 tcggcttcgg acacataaaa ccgggccttg atgttctgcg gtggcagcag cgacagcacc
     4981 gcttgtccgg ccggaatgaa ctcaccgacc tgaaaaaaaa cctcggccac cagcgcatcg
     5041 gctggtgcca cctgtttttt ttgcccggca cgccattggc tttggtgcag cacctcggcg
     5101 gcggcattgg tgctggcctg tgaggcgcgg cgctcgtcgc tgcgtgctgg caggcctgcc
     5161 acctgcagcg ccgcctgtaa ttcggtctct cgtgcctgcg cctgcgccag cactgtcata
     5221 gcatcgtcca cccgtgcttt agcgacaaag ccctgcgtga tgagctgctg ctgacgttgc
     5281 agttcctggc gcgccagcgc agcagcggct tgtgcctggg ccagttgggc ctgcatcacc
     5341 gccaattctt cgctgcgctt gcctttgtcc agattggccg attgggcctg ggcaactgcc
     5401 agccgtgccg ccgcctcatt gctggcggcg ttctcttgtt cagaatccag cacaaatagc
     5461 ggcgcacctt tgacaatgtt ctgacctggc ttgaccgtga cggcatcgag ccgaccggcc
     5521 agcggcgctg caacataaac gtagtcgcct tcagcatagc ctgaccaggc tggtgcgggc
     5581 ttgtcagagc aggccaccag catgctcaca gcagcaatga gcagggctcg cgacctgtga
     5641 tctgtctttt cagtaggcat ggcgtaaata aaacaggtaa tcccccggct ttgccggggc
     5701 gacagtaggg gttttac
//
LOCUS       DAOSLL010000276         5699 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12748_length_5699_cov_4.073884, whole genome shotgun sequence.
ACCESSION   DAOSLL010000276 DAOSLL010000000
VERSION     DAOSLL010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12748_length_5699_cov_4.073884"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..743
                     /locus_tag="PLB25_12420"
     CDS             93..743
                     /locus_tag="PLB25_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42418.1"
                     /translation="MKQMWQAQMARLDGLSLRERIFLFLSILVCCGALVDTLWLSPAQ
                     TAHKQLSQRVEKQGAELQRLRDSLRLSVRPASTGQSSRDELLQISEQTEQINQSVRQL
                     LPNAADAAPLAQALVHLLRRHEGLTLVGTSALAPELAGPGSSPGAGAGTSTLPPGLTR
                     QGVALTVTGAYADLTRYVETLESAMPQVRWGEMSLKSDKGLPELTLKLFLLGELVP"
     gene            740..1138
                     /locus_tag="PLB25_12425"
     CDS             740..1138
                     /locus_tag="PLB25_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42419.1"
                     /translation="MIDVKTLPAMVRQACLVPGFWLLLAGTTDTLAQATRDPTVPPVA
                     AGLAAGAASAGGNTSEAASVSVLVRDGVPYLMVGTRLYAKGQKFGQTQVANITETEVW
                     LLEEGRVRRLPVFNGIERRAANVKPSGLAP"
     gene            1156..2913
                     /locus_tag="PLB25_12430"
     CDS             1156..2913
                     /locus_tag="PLB25_12430"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HOE42420.1"
                     /translation="MNDVSIVVCLTVGLLGAGMLSGCSADRPLRTPSVSEAIRAELPM
                     PDAPAPAVRVPARISDALAEPAQAGGALPTEPKIDLLVNNALAREVFLAIVADTRYSM
                     LMHPGVTGTLSVTLRGVTIAEALEAVRDVYGYDFKIDGRRITVYPPTLQTRIFTINYP
                     QSQRNGSSDLRVSSGGAMQASGTSAGSTAANAGGGSDQSSGSRVSTSSKTDYWSEIST
                     AIKGIVGAGEGRNVITSPQAGIMAVRAMPEELRQVEGFLKAAQVAVERQVVLEAKIVQ
                     VELRDGFQSGIDWSALKNTSNATGAIGVLGGNPETNALIRGVHDNLPGFAADATSLLV
                     DSVALPASGTGLFGLALATEGFQAVLGFLETRGDVQILSSPRIATLNNQKAVLKVGTD
                     DFYVTGVSGGSNSGSSTNSGSNTTLPSITLTPFFSGVSLDVTPQIDQGNNITLHVHPS
                     VTSVTEKTKQVDLGSIGNYRFPLASSSINETDTLVRIQDGKIVAIGGLMQIESSRTSS
                     GLPGSSESIFSSILGNRANSGRKKELVVLIKPTIIRTQQDWDEQSRRSRAALDDMDVA
                     RARIIRIDGKVVHTPIDPQ"
     gene            2916..3842
                     /locus_tag="PLB25_12435"
     CDS             2916..3842
                     /locus_tag="PLB25_12435"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE42421.1"
                     /translation="MYLRHFALREAPFSITPDSAFFFPHDGAQAALNTLLVALRSGEG
                     FLKIVGEVGCGKTVLCRQLLKTLQGECVTAYIPNPDMGPDDLLLALLDELGVDPAPAR
                     RRDTSGHLRLSRHQLMTLLANCLFRHAQDGQRVVVCIDEAQAIPLRTVESLRLLSNLE
                     TEKQKLLQLVLLGQPELDVKLTNPEIRQLLQRITFSEYLGPMRSAQVPMYLVHRLATA
                     AQGEATDLQVFQADAARTIARLSGGVPRLVNVLAHKCLMLAFGENTHRVTVAHVRLAA
                     ADTPGVPSMTSGWRVWQQRLRQSWLARTGKVA"
     gene            3839..4933
                     /locus_tag="PLB25_12440"
     CDS             3839..4933
                     /locus_tag="PLB25_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE42422.1"
                     /translation="MSVINKVLRDLDSRQTAEQPVRHADAARLYPKGLVNGVVVVGGE
                     SGPGRARPLIDWRVALVTLLVLLAGAAGLMWWLQPPTVIKPAWLVPSTPAMPTVMPTA
                     VPTSNPRLPMPVAGSVEAPASRQTAAALAVAKPRADAVASPASIAVPATAAVKVPRGV
                     AVAGPAISKPMSGALASEPPPDLISAPPPASATNVSEARSERAGTAELAAQAQLLWNN
                     GDRRAALALLHNALLRLEQAAANPGALAQLAREHARMSLALGQPADALAMLERLEPQL
                     AGVADIWAMRGNAAQRLGQHAQAVAAYQRGLALRPDESRWLLGAAVSLAAMGQTSPAA
                     ELAEKARALKALPPDVANYLRQLGVAVRSD"
     gene            4980..>5699
                     /locus_tag="PLB25_12445"
     CDS             4980..>5699
                     /locus_tag="PLB25_12445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HOE42423.1"
                     /translation="MLKRVGVQQLQLGMHLKGFCGSWMDHPFWRTGFVLKDPNDIALI
                     LASSIKEVWIDCDLGLDVPAGEPAVCEPDPPAVAAPPTPTAARPPPLRETTPVTAAQE
                     VERASRICLEAKAAVVSMFEEVRMGKAVDVGGARQLVEDISDSVARNPGAIISLARLK
                     TADDYTYMHSVAVCAMMVALAKQLGLDEPQTRACGMAGLLHDLGKVAMPTEVLNKPGK
                     LTNAEFDIMKTHTTLGFDTISR"
BASE COUNT         1056 a         1696 c         1836 g         1111 t
ORIGIN      
        1 acgtgcgcag gcacgtttgg agccgcggct ttcgccctac ccagacgcgc cgccaacggc
       61 ccactcgggc gcgtccaact gaggattcaa ggatgaagca aatgtggcaa gctcagatgg
      121 cgcggctgga tggcttgagt ttgcgcgagc ggatttttct atttttatcg atcctggtgt
      181 gttgtggcgc gctggtggac accctgtggc tgtcaccggc gcagactgcc cacaagcagt
      241 tgtcgcagcg ggttgaaaag cagggcgcag agttgcagcg cctgcgcgac agcttgcggc
      301 tgagcgtccg tcccgccagc acaggccaga gttcgcgtga tgaattgctg caaatcagcg
      361 aacagaccga gcaaatcaac cagtcggtgc ggcagttgtt gccgaacgcc gctgatgccg
      421 cgccgctggc tcaggcgctg gtgcacctgc tgcgccgaca tgagggtttg acgctggtgg
      481 gaaccagcgc cttggcgccc gagctggccg gaccaggcag cagccccggt gctggtgcag
      541 gcaccagcac attgccgccc ggtttgacgc gccagggggt ggctttgacg gtgaccgggg
      601 cgtatgccga tttgacgcgc tatgttgaaa cgcttgagag cgccatgcca caggtgcgct
      661 ggggcgagat gagcctcaaa agcgacaagg gcttgccgga actgacgctc aaactgtttt
      721 tgctcgggga gcttgtgccg tgattgatgt caagacgctg cctgcaatgg tccgccaggc
      781 gtgcctggtg ccgggctttt ggttgctgct ggcaggcacc accgacacat tggcgcaggc
      841 aacgcgcgat cctaccgtgc cgccggttgc cgcagggttg gcggctggcg ctgcaagcgc
      901 tggtggcaac accagcgaag ccgccagcgt ttcggttctg gtgcgtgacg gtgtgcccta
      961 cctgatggtt ggcacccgcc tgtacgcgaa aggtcagaag tttggccaga cgcaggttgc
     1021 aaacatcacc gaaaccgagg tctggctact cgaagaaggc cgcgtgcgcc ggctgccggt
     1081 gttcaatggc attgagcgcc gcgccgcaaa tgtcaaacca tcaggtcttg ccccatgaac
     1141 cgaagagctc tcaccatgaa tgatgtgtcg attgttgtgt gcctgacagt tgggctgttg
     1201 ggcgcaggca tgctcagtgg ctgcagtgcc gaccggccgc tgcgcacacc cagtgtcagc
     1261 gaagccattc gtgccgagct gccgatgcct gatgcaccgg caccagcggt gcgggtgccc
     1321 gcgcgtattt cagatgcact ggccgagccc gcacaagctg ggggcgcatt gccaaccgag
     1381 cccaaaattg acctgctggt caacaatgcc ctggcgcgcg aagtgttcct ggcgatcgtc
     1441 gcagacaccc gctacagcat gctgatgcac cctggggtga ctggcacttt gtcggtgact
     1501 ttgcgtgggg tcacgattgc cgaggcactt gaggcagttc gggatgttta cggctacgac
     1561 ttcaagatcg atggtcggcg catcacggtt tacccgccaa ccctgcaaac ccgaattttc
     1621 accatcaatt acccgcaatc tcagcgcaac ggcagcagcg acttgcgggt gtcgtctggc
     1681 ggggcgatgc aggcatccgg caccagcgcg ggcagcacgg ctgccaacgc gggtggcggt
     1741 tccgaccagt ccagcggcag tcgcgtcagc accagttcca aaaccgatta ctggagtgaa
     1801 atttccaccg ccatcaaagg catcgtcggg gcaggtgagg gtcgcaacgt gatcaccagc
     1861 ccgcaggcag gcatcatggc ggtgcgcgcc atgccggaag aactgcgaca ggttgagggg
     1921 tttctcaagg cggcccaggt tgcggtggag cggcaggtgg tgctggaggc caagattgtg
     1981 caggttgaat tgcgcgacgg gtttcaaagc ggcattgact ggtcggcttt gaaaaacacc
     2041 agcaatgcca ccggtgcgat tggcgtgctg ggtggcaatc cggaaaccaa cgcgctgatt
     2101 cgcggggtgc atgacaacct gcccggtttt gctgctgatg ccacatccct gctggtcgat
     2161 tcggttgctc tgccagcttc cggcaccggc ctgtttggcc tggcgctggc aaccgaaggg
     2221 tttcaggcgg tgctgggttt tctggaaacc cggggtgacg tgcaaatact gtcgagcccg
     2281 cgcattgcca cgctgaacaa ccaaaaagcc gtgctcaagg tcggtaccga tgacttttat
     2341 gtcacaggtg tcagcggcgg cagcaattca ggcagttcga ccaattccgg ttcgaacacc
     2401 accttgccaa gcattaccct gacaccgttt ttctctggcg tttcgctcga tgtgacgccg
     2461 caaattgatc agggcaacaa catcaccttg catgtgcatc cgtcggtgac ttcggtgacc
     2521 gagaagacca agcaggttga tctgggttcc attggcaatt accgtttccc gctggcatcc
     2581 agcagcatca acgaaaccga caccttggtg cgcattcagg acggcaaaat cgtcgccatt
     2641 ggtggcctga tgcaaatcga gtccagccgc acgtcttcgg gtttgcctgg ctccagtgag
     2701 tcgattttct caagcattct gggtaaccgt gccaacagcg ggcgcaaaaa ggaattggtg
     2761 gtgctgatca aacccaccat catccgtacc cagcaggact gggatgagca aagccgccgc
     2821 agccgtgcgg cgctcgacga catggatgtc gcgcgtgccc ggatcatccg catcgacggc
     2881 aaagtggtgc acacgccgat tgatccccaa tagccatgta tttgcggcat ttcgccctgc
     2941 gagaggcacc gttttcaatc accccggaca gtgctttttt tttcccgcat gacggtgcgc
     3001 aggctgccct caacaccttg ttggtcgctt tgcgcagtgg cgaaggcttc ctgaaaattg
     3061 tcggcgaggt tggttgtggc aaaacggtgt tgtgccgcca actgctcaag accttgcagg
     3121 gcgaatgcgt caccgcctac attcccaacc cggatatggg gccggacgat ctgctgctgg
     3181 ccttgctcga tgaactgggt gttgatccgg caccggcccg tcggcgtgac acttccgggc
     3241 atttgcgcct gagccgccat caactcatga cgctgctggc caattgcttg ttcagacatg
     3301 cccaggatgg gcaacgggtg gtggtgtgca tcgatgaagc gcaggcgata ccgctgcgaa
     3361 ccgtcgaaag cctgcggctg ctgtccaacc tggaaaccga aaagcagaaa ctgctgcaac
     3421 tggtgctgtt ggggcagccc gagctcgatg tcaagctgac caatccggaa atccggcagt
     3481 tgctgcagcg cattaccttc agcgaatacc tgggtccgat gcgctctgca caggtgccga
     3541 tgtacctggt gcaccggctt gccacagcgg cgcagggcga ggcgactgat ctgcaggttt
     3601 ttcaggctga tgcggcccgg accatagcgc ggctcagtgg cggtgtgccc cgacttgtga
     3661 atgtgctggc gcacaaatgc ctgatgctgg cgtttggcga gaacacccat cgggtgaccg
     3721 ttgcacatgt caggctggcg gctgcggaca cgccgggcgt accgtcaatg acctctggct
     3781 ggcgtgtctg gcaacagcgg ttgcggcaaa gctggctggc acgcacagga aaagtggcat
     3841 gagcgtgatc aacaaggtgc tgcgcgacct ggacagtcgg caaactgctg agcaacctgt
     3901 tcggcatgca gacgctgccc gtctctaccc caaagggctg gtcaatgggg tggttgtggt
     3961 gggtggcgaa tccggccctg gccgtgccag accgctgatc gactggcggg ttgcgctggt
     4021 cacgctgctg gtgttgctgg cgggcgcggc tgggctgatg tggtggctgc agccaccaac
     4081 cgtcatcaag ccagcctggt tggtacccag caccccggca atgccgactg tgatgccaac
     4141 ggctgtgccg acatcaaatc cacggctgcc gatgccagtg gctggcagtg tcgaggcccc
     4201 ggcgtcgcgc cagactgctg cagccttggc ggtagccaaa cctagggcag acgccgtcgc
     4261 ctcacctgca tcgattgccg tgccagcaac agcggctgtc aaagtcccgc gtggggtggc
     4321 tgttgcaggg ccagcgatca gcaagccaat gtcaggggcg ttggcatctg aaccgccccc
     4381 ggatttgatt tctgcaccgc ctccggcaag tgccaccaat gtgtccgaag ccaggtctga
     4441 gcgagctggc acagcggaac tggccgcgca ggcacagctc ctgtggaaca acggtgatcg
     4501 ccgcgccgcc cttgccctgc tgcacaacgc attgctgcgg ctggaacagg ctgcggccaa
     4561 ccctggcgcg ctggcccagt tggcacgcga gcacgcccgc atgagcctgg ccctgggcca
     4621 gccagccgac gcactggcaa tgctggagcg cctggagccg caactggctg gcgttgccga
     4681 catttgggcg atgcgtggca atgctgcgca acgcctgggg cagcatgcgc aggcagtggc
     4741 ggcctaccag cgcggcctgg ccctgcgccc ggacgagtcg cgctggctgt tgggtgcagc
     4801 agtttccctg gcggcgatgg gtcagaccag cccggcggcc gaactggccg agaaggcgcg
     4861 ggcgctcaag gctttgccgc cagatgtggc aaactacctg cgtcagttgg gcgtggcggt
     4921 tcgttccgat tgacattcgt ccgctttaac cacttcgcct gcgttcaccc ttatccatca
     4981 tgctcaagcg cgtcggtgtt caacaactgc aactgggcat gcaccttaag gggttttgtg
     5041 gctcctggat ggatcatccc ttttggcgca ccggctttgt cttgaaagat cccaatgaca
     5101 tcgcgctgat tttggccagc agcatcaagg aggtctggat cgactgcgac ctgggtctgg
     5161 atgtgccagc cggtgagcct gctgtctgtg agcccgaccc acctgccgtt gcggccccac
     5221 ccacgccgac cgctgcccgt ccaccacccc tgcgtgagac cacgccggtc acggcggcgc
     5281 aagaggttga gcgtgcttcg cgcatttgcc tggaggccaa ggctgccgtg gtgtcgatgt
     5341 ttgaagaagt ccgcatgggc aaagcggtgg atgtgggtgg tgcccgccaa ttggtggagg
     5401 atatttctga ctcggtggcg cgcaatccag gtgccatcat cagccttgcg cggctgaaaa
     5461 cagcggacga ctacacctac atgcattcgg tggcagtgtg cgccatgatg gtggcgctgg
     5521 ccaagcaact gggtctcgac gagccgcaaa cccgcgcctg cggcatggct ggcctgctgc
     5581 atgatctggg caaggtggcg atgccgaccg aggtgttgaa caagcccggc aagttgacca
     5641 acgccgagtt cgacatcatg aaaacccaca cgacgttggg atttgacacg atctcgcgg
//
LOCUS       DAOSLL010000277         5670 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12824_length_5670_cov_4.745859, whole genome shotgun sequence.
ACCESSION   DAOSLL010000277 DAOSLL010000000
VERSION     DAOSLL010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5670
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12824_length_5670_cov_4.745859"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /locus_tag="PLB25_12450"
     CDS             <1..901
                     /locus_tag="PLB25_12450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03016 family PEP-CTERM system-associated
                     outer membrane protein"
                     /protein_id="HOE42424.1"
                     /translation="WTADLTRQSVSYSEGRATESDTINFGPLYNVNPQIQVYAKVGRE
                     ANNFTGETKQSYNTSTLGVKWVPSTLTTFNLERSHRSFGDAYNLSLEHRTARTVWKFT
                     DNKDVSSTSGQTATSSKGSMYDLLFSQFASTEPDPAARARLVDDYLLTNGISATANAD
                     NSFQTAAISLQRRQELSFALLGVRDAITFVASRSESSRLDTVSTAVDDLAAGQVRQQS
                     LSLQFAHRLTPDYALGLLFSWQKNSGNASLSDTNLRSTNVIVGGKLGKKSTVLLGARR
                     TVADGDSAPYSESAVIGQINVQF"
     gene            905..2011
                     /locus_tag="PLB25_12455"
     CDS             905..2011
                     /locus_tag="PLB25_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA-associated ATPase"
                     /protein_id="HOE42425.1"
                     /translation="MYEDFYGLKSKPFQINPDPSFYFGSKQHRRAKAYLEYGVVRNEG
                     FIVITGEVGAGKTTVVRGLLGGLDAEKVVAANLVTTQLDAEDTLRMVGSAFGVRVKDI
                     SKADLLMALEAFFLNQTSQGKRCLLIVDEAQNLTARAVEELRMLSNFQYDQQALLQTF
                     LVGQPEFRAILQSPGLKQLRQRVTASCHIGPLDQEETQGYIEHRLRCAGSTGRPTFDE
                     AAVEKIFKSSDGIPRRINLLCDRLLLLGFLSGKDAFGLEDVVEVVDEIQEESGVSTEP
                     TPADKSGWSNSTLQASVDAGPLDVDLSKLQVTAALARTISNQADALGIEQFDARLRRL
                     EGSALRLERISLETLAMLQKLVKAATKPPEGRGQ"
     gene            2038..2901
                     /locus_tag="PLB25_12460"
     CDS             2038..2901
                     /locus_tag="PLB25_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HOE42426.1"
                     /translation="MPSSARVNALTIDVEDYFQVSAFAPHIARSEWSERECRVERNIH
                     RILELLTTHNTKATFFTLGWIAERYPQLVRLIVAQGHELASHGYGHERATDQTEDVFY
                     ADIHLAKLILEDLSGHEVSGYRAPSFSIGPGNLWAFDCLARAGYHYSSSIYPIAHDHY
                     GMPDSPRFAYEVRPGLMEIPITTMRMFNRNLPSSGGGYFRLLPYALSRWMLNRVNALD
                     QQSGIFYFHPWEIDTGQPRVAGISAKTRFRHYVNIHRMEGRLKRLLSDFNWGRMDSVF
                     LNQPVTAQVHA"
     gene            3020..4057
                     /locus_tag="PLB25_12465"
     CDS             3020..4057
                     /locus_tag="PLB25_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family PEP-CTERM system-associated
                     protein"
                     /protein_id="HOE42427.1"
                     /translation="MLSIQKLHPDDSATEQEWDAFVMACPEATFFHRAGWQRIVRDVF
                     HHDTYFLYAQDNGAIQAVLPLGHVNSWLFGNSLTSLPFAVYGGVASVNPVAAQALVRE
                     AQNIAQRLGVAHLELRNVKPQQIDWPTQDIYVTFRKEILPDEQANMLAIPRKQRAMVR
                     KGIKNGLVSQIDETVHAFFALFADNVRRHGTPALPKKYFKALQAEFGADCEILTVTAE
                     DGRPLSSVLSFYFRDEVLPYYAGDDEAARDLAANDFKYWELMRRACARGLKVFDYGRS
                     KRGTGPFAFKRNWGFEPTPLNYEYCLYKRDSIPQNNPNNAKYKLMISVWRRMPIGLAN
                     WLGPFVVRNLG"
     gene            4064..5293
                     /locus_tag="PLB25_12470"
     CDS             4064..5293
                     /locus_tag="PLB25_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03087 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HOE42428.1"
                     /translation="MGKLLYLVHRLPYPPNKGDKVRSYHLLKHLAQNHQVFLGTFIDD
                     PVDEAYVPLVQDICPHNYIARLHPRSAKLRSLAALPTPQALSLRYYQNAGLQTWVNNT
                     LASQDIDAIVIFSSVMAQYVASVPGNSKVPMLVDFVDVDSVKWTQYAPQHRWPLSWLY
                     QLEGRRLLAFERTTALRAKHSFFVTDNEVALFRQLAPESADRVEAISNGVDADYFSPD
                     PACTSPFAQQTLTAAQVPVVFSGAMDYWPNIDAVSWFVAEVLPDLLKHWPSLCFYIVG
                     RSPVPAVMALAGPSVVVTGTVPDVRPYLQHAAVVVAPLRVSRGIQNKILEAMAMARPV
                     VASTSCVEAINAHVDRELMAATSASDFVVQINSLLCDPARSADIGQAGRLRVLQHYSW
                     SAHLSKIDRHLHPKASA"
     gene            5290..>5670
                     /locus_tag="PLB25_12475"
     CDS             5290..>5670
                     /locus_tag="PLB25_12475"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HOE42429.1"
                     /translation="MTPLAQTQSGQNWRQALPVFLLLLALTLTLFQGTGSAIVTIWLR
                     SDTFAHGFLVPPIVIWLVWRRHQMLADIPPQPSYWGMLMFAGAAFAWLLGDLVAVNAV
                     AQLALVAMLVLLVPALLGFAVARAV"
BASE COUNT         1217 a         1613 c         1520 g         1320 t
ORIGIN      
        1 ttggactgct gaccttacac ggcagagcgt gtcgtacagt gaagggcgag cgacagagtc
       61 ggacaccatc aactttggcc cgctctataa cgtcaatccg cagattcagg tctacgccaa
      121 agttgggcgt gaggccaaca acttcacagg cgaaaccaag caaagttaca acaccagcac
      181 cctaggtgta aagtgggtgc catcaacgct gaccacgttt aatttggagc gtagccatcg
      241 ctcgtttggg gatgcctata acttgtcact tgagcaccgt accgcacgca cggtctggaa
      301 attcaccgac aacaaggacg tgtcctcaac atcagggcag accgctacca gcagcaaagg
      361 ctcaatgtat gacctgttgt ttagccagtt cgcctcgact gagccagatc cggccgcacg
      421 tgcgcgtctg gttgatgact atttgctaac taatggcatc agcgccaccg ccaatgctga
      481 taacagtttc cagacagcgg ccatttcttt gcaacgtcgc caagagttgt cttttgctct
      541 gctgggggtt cgtgatgcca tcacctttgt cgcctcgcgc agcgaatcca gcaggcttga
      601 cacggtatca acggctgttg atgaccttgc cgcgggtcag gtccgtcagc agtccttgag
      661 cctccagttc gcccaccgat tgacacctga ctacgcattg ggcctgctgt tttcgtggca
      721 aaaaaattct ggcaatgcca gtttgtcaga cacaaacctg aggtcgacca atgtcatcgt
      781 tggtggcaaa cttggcaaga agtcgaccgt attgctcggt gcgcgccgaa ccgtcgcaga
      841 cggtgacagt gcgccctact ccgaaagcgc cgttattggt cagattaacg tgcagttcta
      901 gcccatgtat gaagattttt acgggttaaa aagcaagcct tttcagatca atcccgaccc
      961 cagtttttac ttcggcagca agcagcatag gcgtgccaag gcctacctgg aatacggtgt
     1021 ggtgcgaaac gagggtttca ttgttatcac aggtgaagtc ggtgccggca aaaccacagt
     1081 tgtgcgcggt ctgcttgggg gacttgatgc cgaaaaggtg gttgctgcca atctggttac
     1141 cactcagctc gatgccgaag acacgttgcg catggtggga tcagcctttg gcgtgcgcgt
     1201 caaggacata tcgaaagccg acttgctgat ggctttggag gccttttttc tgaaccagac
     1261 cagccagggt aaacgctgcc tgctgatcgt cgacgaggcg caaaacctca ccgcgcgagc
     1321 cgtcgaagag ttgcgcatgc tgtccaattt tcagtatgat cagcaagcgt tgctgcagac
     1381 ctttcttgtc ggacaacccg agtttcgggc tattttgcaa agtccaggcc tgaaacaact
     1441 cagacaacgg gtaacagcca gttgtcatat cgggccattg gatcaggaag aaacgcaggg
     1501 gtacatcgaa catcggctca gatgcgcagg ctccacgggt cgccctacat ttgacgaagc
     1561 ggccgttgaa aagatattca agtcctccga tggcattcca cggcgaatca atctactttg
     1621 tgaccggttg ctgctgcttg gttttttgag tggcaaagac gcattcgggc ttgaagatgt
     1681 ggtcgaagtc gttgatgaaa ttcaggagga atccggcgtg tccacagagc cgacacccgc
     1741 agacaaatcc gggtggagca attcgactct gcaagcctct gtcgatgcgg gtccgttgga
     1801 cgttgacttg agcaaattgc aggtgaccgc tgctctggcc cgcactattt ccaaccaggc
     1861 ggatgctctg ggcattgagc agtttgatgc ccgattgcga agacttgaag gcagtgcgct
     1921 gcgcttggag cgaatcagcc tcgaaacgct ggccatgctg caaaagctgg tcaaggcagc
     1981 gaccaagccc cccgaaggga gaggtcaatg aacgattccg gaaccgggga cattgccatg
     2041 ccatcctcag cgagggtgaa tgccttaacc attgatgttg aagactactt tcaagtctct
     2101 gcgttcgcgc cccatattgc gcgctccgag tggagcgaac gtgaatgccg ggttgagcgg
     2161 aatattcacc gtattcttga gttgctgacc acccacaaca ccaaggccac tttttttacc
     2221 ttgggctgga tcgccgagcg ctacccgcaa ctggtcaggc tgatcgtggc acaggggcac
     2281 gaattggcta gccacgggta tggtcacgaa cgggccaccg atcaaaccga agatgttttt
     2341 tatgccgaca ttcacctagc caaattgatc cttgaggact tgtccggcca cgaggtcagc
     2401 ggttaccgtg ccccaagctt ttcaattggc cccggcaacc tgtgggcatt tgactgcctg
     2461 gcccgggccg gctaccacta cagctccagc atttacccga ttgcgcacga ccactacggc
     2521 atgcccgact ctccgcgttt tgcctacgag gtgcgtcccg ggttaatgga aatccccatt
     2581 accaccatgc gcatgttcaa tcgcaatctt ccctccagcg gaggcgggta tttcaggctg
     2641 ttgccctatg cgttgtcgcg ttggatgctt aaccgcgtca atgcgcttga tcagcaatca
     2701 ggcatctttt attttcatcc gtgggagatt gacacggggc aaccgcgtgt cgctggtatt
     2761 agcgcaaaga cccgctttcg ccattacgtc aatattcacc gaatggaagg tcggctcaaa
     2821 cgcctgctca gcgatttcaa ttggggccgt atggacagcg tctttttgaa ccagcccgtc
     2881 acagcacagg ttcacgcctg aatgtcatgg gctcactgcc gcccagcata atgcaacgag
     2941 ctgacgctac ggtgttgagt cggccacgaa gtacgacggc gcgaccaagt tttttcacgt
     3001 caaccgcctg acaagccaca tgctcagcat tcaaaaactc caccctgacg actcagctac
     3061 tgagcaagag tgggatgcat ttgttatggc gtgcccagag gccacatttt ttcatcgggc
     3121 cggctggcag cgcattgtgc gcgatgtctt tcatcacgat acctattttt tgtacgccca
     3181 ggacaatggg gccatacaag ctgttctgcc gctggggcat gtcaatagct ggctgttcgg
     3241 caattcactc accagcctgc cttttgcagt ttacggtggc gtggcttcgg tcaatcccgt
     3301 ggctgcgcag gccctggtgc gggaagcgca gaacattgca caacgccttg gcgtggcaca
     3361 cctcgagttg cgaaacgtca aaccccagca gattgattgg cccactcagg atatctacgt
     3421 cacctttcgc aaggaaatct tgcctgacga acaggccaac atgctggcca taccccgcaa
     3481 acagcgggcc atggtgcgca aaggcatcaa gaacgggtta gtcagccaga tcgacgaaac
     3541 cgttcacgca ttctttgcct tgtttgctga caatgtccgc cgacacggca cgcctgcact
     3601 gcccaagaag tacttcaaag ccctccaagc tgaatttggg gctgactgcg aaatacttac
     3661 cgttactgcc gaagatggcc gtcctctgag cagcgttttg agcttttact ttcgcgacga
     3721 agtgttgccc tattacgcag gcgatgacga agctgcgcga gaccttgcag ccaatgattt
     3781 caagtattgg gaactgatgc gccgcgcctg cgcccgaggt ctcaaagtat ttgactatgg
     3841 tcgcagcaag cgcggcaccg gaccgtttgc tttcaaaaga aattggggtt tcgaacccac
     3901 ccccctgaac tacgaatact gcctttataa acgtgacagc attccacaga acaaccccaa
     3961 caatgccaaa tacaagctca tgatcagcgt ctggcggcgc atgccaattg ggctggccaa
     4021 ttggctggga ccgtttgttg ttcgcaacct tggttgaaag tgcatgggca aactgcttta
     4081 cctggtgcac cggctgccct acccgcccaa caaaggcgac aaggtacgct cttaccattt
     4141 gctcaagcac cttgcccaga accatcaggt gttcctgggc accttcattg atgacccggt
     4201 tgatgaagcc tatgtgcctt tggtacaaga catctgcccg cacaactaca tcgcaagact
     4261 tcatccgcgt tctgccaaac ttcgcagtct tgccgcactg ccgacgccac aggctttgag
     4321 tttgcgttac taccaaaatg ctggtttgca gacctgggtg aacaacacct tggccagcca
     4381 ggacattgat gccatcgtta ttttttcatc ggtcatggcg cagtatgttg cttcagtgcc
     4441 gggcaacagc aaggtgccta tgctggtcga ctttgttgat gttgactcgg tcaaatggac
     4501 acaatatgcg ccccagcacc gctggcccct gtcatggctt taccagcttg aagggcggcg
     4561 gttgctggcc tttgagcgga cgactgccct gcgggccaag cattcctttt ttgtgacaga
     4621 caacgaagtc gccttatttc gtcaactggc ccccgagagt gcagaccgcg tggaagcgat
     4681 cagcaatgga gtcgatgccg attacttttc gccagatccc gcatgcactt ctccttttgc
     4741 tcagcagacc ctgacagcgg cgcaggttcc cgtggtgttt tcaggtgcca tggactattg
     4801 gcccaacatt gatgcggtaa gctggttcgt cgcagaggtt ctaccagacc ttctcaaaca
     4861 ctggccatcg ctctgttttt atattgttgg gcgcagtccg gttccggcag tgatggcgct
     4921 ggccggccca tcagtggtgg tcacaggcac tgtccccgat gtgcgcccat acctgcagca
     4981 cgctgctgtt gtcgtggccc cgctacgggt atcgcgaggc atccagaaca aaattcttga
     5041 agccatggcc atggcccgac cggtcgtcgc ctccacctcc tgtgttgaag ccatcaacgc
     5101 ccatgtcgat cgcgaactta tggccgcaac aagtgccagc gactttgttg tgcaaatcaa
     5161 ctccctgttg tgcgaccccg cgcgctcggc cgatattggc caagctggcc gactgcgggt
     5221 actgcaacac tacagttggt ctgcccattt gtccaagatt gaccgccacc tgcatccaaa
     5281 ggcttctgca tgacccctct ggcacaaacg caatccgggc aaaactggcg gcaggcctta
     5341 cctgttttct tgttgctgct ggcgctgaca ttaactttgt ttcaaggcac aggcagtgcc
     5401 atcgttacca tctggcttcg ctcggatacc tttgcccacg ggtttctggt gccacccatt
     5461 gtgatctggc tggtgtggcg caggcatcaa atgcttgccg acataccgcc acagccaagc
     5521 tactggggca tgctgatgtt tgccggtgcc gcctttgctt ggctgctggg cgacctggtc
     5581 gccgtcaacg ctgtagccca attggcgctg gttgccatgc tggtgctgtt ggtgcccgcc
     5641 ctgttggggt ttgccgtggc tcgggctgtc
//
LOCUS       DAOSLL010000278         5666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12837_length_5666_cov_5.318125, whole genome shotgun sequence.
ACCESSION   DAOSLL010000278 DAOSLL010000000
VERSION     DAOSLL010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12837_length_5666_cov_5.318125"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            240..3347
                     /gene="ygfK"
                     /locus_tag="PLB25_12480"
     CDS             240..3347
                     /gene="ygfK"
                     /locus_tag="PLB25_12480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HOE42430.1"
                     /translation="MSDIMRPVPFEGLLQRIFEEFRVSRSIFDIAESQFYRKADDRLI
                     EVFGETCETPVGPAAGPHTQLAQNIVASWLVGGRFIELKTVQIMDRLEIEKPCIDAAD
                     EGYNTEWSSEYTLPKAFDEYLKAWFALHLLEAVFDPRAEGEANSFSFNMSVGYNLDGI
                     KQAPMQAFIDSTIDASAHPKFAEYQRILDKWVNDESFIESLGLQSRKAQLQSLPARIS
                     ARLVKGVTLSTMHGCPPDEIESICRYMLEVKSINTFVKLNPTLLGFQRVREILDTCGF
                     DYLIVKEASFEHDLKLDQACAMLRRLLDLSQQKNLSFGVKLTNTLGAVNHKGALPGEE
                     MYMSGRALFPLSINVAALLSREFDGRLPISYSGGASLYNIREIFETGIRPITMATDLL
                     KPGGYLRLLECARALDASDAWQMGQVDVARLELLAAKAITADYTQKAWRSKDEIDTGS
                     ALPLTDCYVAPCVSACAIHQDIPEYIRLVSEGRYADALEVIYRTNALPAITGHICDHL
                     CQHNCTRLDYEGPLNIRELKKIALEKGWDEYRRRWHKPAGTGSRNAVAVVGMGPAGLA
                     AGYFLARAGHAVTLFDREQAAGGVPRHVIPRFRSPGDLVQHDINFVAEHGVKFVYGCD
                     PQLSVDKLKAQGFEYVLIGIGTDKNSGIQLGGDNRNLYKSLPFLRRFNDGEKLDLGRH
                     VAVVGAGNTAMDCARAALRVPGVEDVTVIYRRSFNEIPAYREEYEEAVADGVRFHFLT
                     NPERFDADGTLTCRVMELGAPDGKGRRRPLPTDRTIALKMDALITAIGEQADCDVLKA
                     MGVPLGEDGWPAVNQASSETTRQNVFLIGDVKQGPSSIVGAIGDARRATDEILKRENL
                     KSHYGQKFWLNADPNEITQRKGAVVVNFVKKDAFDAFVKQEGQRCLECNYVCAKCVDV
                     CPNRANISIAVPGFKDRAQTLHIDAYCNECGNCAQFCPWQGRPYKDKITIFSLPQDFA
                     NSSNPGFLVEAGKLTVRQDGKVSELTIDSAGAVKDAPPELADLCRIASRVVSHHGYLL
                     SAVEA"
     gene            3361..4689
                     /gene="ssnA"
                     /locus_tag="PLB25_12485"
     CDS             3361..4689
                     /gene="ssnA"
                     /locus_tag="PLB25_12485"
                     /inference="COORDINATES: protein motif:HMM:NF005540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HOE42431.1"
                     /translation="MLILRNLTALQVEPATVWGDVQIAIDDTRILEVGAGLAAKYPQA
                     QVLDLKGKLVMPGIVCAHNHFYSGLARGIMANILPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTAVIDHHASPNFIAGSLATLRQGFLKAGLRGMTCYETTDRN
                     GGEKELRAGVDENLAFARLIDAAKAKGDEPYLMEAHLGAHAPFTVSDAGLEMLGDAVK
                     TTGRGLHIHVAEDRYDVSHSHHRYSQDPIERLAAHGLIDGKTLIGHGLYLSDADIAIL
                     NDRDAFLVHNARSNMNNNVGYNHKLAAVRNLALGTDGIGADMFEELKFAYFKHRDAGG
                     SLWPDSFARFLWHGNTLLERNFGARFGRIEAGYQADLTICSYDSPTPLVAQNVGGHLA
                     FGMNSGSVDSVMVNGQMVYQNRAFPFDVEPIYAQARKVAAQLWQRMDALV"
     gene            4932..5105
                     /locus_tag="PLB25_12490"
     CDS             4932..5105
                     /locus_tag="PLB25_12490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42432.1"
                     /translation="MIDECRAACSASPAAHPDVFLKRLLRLPGWDAEYAQRVLTYFFG
                     PQGVTPQPDSIAA"
     gene            5102..>5666
                     /gene="yqeC"
                     /locus_tag="PLB25_12495"
     CDS             5102..>5666
                     /gene="yqeC"
                     /locus_tag="PLB25_12495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HOE42433.1"
                     /translation="MTGIHFLCAPGNSDFIARCVGADVPSVVSLVGAGGKTSTLFWLA
                     QALAGCGQRVLLTTTTRMFRPEPGRGATLLIEPERAQRLAALRGLPNAPGIVALYSQI
                     DANEGKVIGFEPDEIDELKAMAVADVILVEADGARHCALKAPAAHEPCIPRGSNAVIA
                     LTGAAPLGCPANPADIHRWPQFAAITGL"
BASE COUNT         1199 a         1647 c         1671 g         1149 t
ORIGIN      
        1 actcctgcac aaacagtcgg cgcctatttg aatttgtagg agtcgcgccc tcgcggcgaa
       61 tgactttgaa tgtttgaatt cttcgcatga tatgcaaaga cttgacttgt tagagaccaa
      121 ggcgattggc caggcgcgat ttcatgtgag cattgccttt ggcccgaaac ttgcagccaa
      181 gccaccgaac accacacttt gcgtgtgccc agcctatcac tcagctattg gagatcatca
      241 tgagcgacat catgcggccc gttccgttcg agggactgtt gcaacgtatc tttgaagagt
      301 ttcgggtcag ccgctcaatc ttcgacattg ccgagagcca gttctaccgt aaggccgatg
      361 atcgcctgat cgaggttttt ggcgagacct gtgaaacacc ggttggcccg gcggccggac
      421 cgcacacgca actggcgcag aacatcgtgg cttcctggct ggtgggtggg cgcttcattg
      481 aactcaagac ggtgcagatc atggaccggc ttgaaatcga gaagccatgc atcgatgccg
      541 ccgatgaggg ctacaacacc gaatggtctt cggaatacac cttgccgaaa gcctttgacg
      601 aatacctgaa ggcctggttt gcgctgcatc tgctcgaagc tgtgtttgac ccgcgcgctg
      661 agggcgaagc caattcattc agtttcaata tgagcgtggg ctacaacctc gacggtatca
      721 agcaggctcc gatgcaagcc ttcattgaca gcaccatcga tgcctcggcg catccaaagt
      781 ttgccgagta ccaacgcatt cttgacaaat gggtcaacga tgaaagcttt atcgaaagcc
      841 tgggcctgca aagccgcaag gcgcaactgc aaagcctgcc agcacgtatt tcggcgcggc
      901 tggtcaaagg tgtcacgtta tcgacgatgc acggctgccc gcctgatgag atcgaatcga
      961 tctgccgcta catgctcgaa gtcaagtcga tcaacacttt cgtcaagctc aaccccaccc
     1021 tgctgggttt tcagcgggta cgcgaaattc tcgacacctg cggctttgac tatctgatcg
     1081 tcaaggaagc ttcgtttgaa catgacctca agcttgatca ggcctgcgcc atgctgcgcc
     1141 gcctgctgga tttgagccag caaaaaaatc tcagcttcgg tgtcaagctg accaacacgc
     1201 tgggtgcggt caaccacaag ggggccctgc cgggcgagga aatgtacatg tcgggtcggg
     1261 cgctgtttcc gctgtcgatc aacgtggcgg cgctgctgtc acgcgaattc gacgggcggt
     1321 tgccgatttc ctactcgggt ggtgccagcc tgtacaacat tcgcgagatc ttcgagaccg
     1381 gcatccgccc catcaccatg gccaccgatc tgctcaagcc cggcggctat ctgcggctgc
     1441 tggaatgcgc gcgcgcgctg gacgcgtcgg atgcctggca gatgggccag gtcgatgttg
     1501 ccaggctcga actgctggca gccaaggcga tcactgctga ctacacgcaa aaggcctggc
     1561 gctccaaaga cgaaatcgac actggcagtg cgctgccgct gactgactgt tacgttgccc
     1621 cctgcgtgtc cgcgtgcgcc atccatcagg acatccctga gtacattcgc ctggtcagcg
     1681 aagggcgcta cgctgacgcg ctggaagtca tctaccgcac caatgcgctg ccggcgatca
     1741 ccgggcacat ttgcgatcac ctgtgtcagc acaactgcac gcgactcgat tacgaaggcc
     1801 cgctcaatat ccgcgaactg aaaaaaatcg cgcttgaaaa aggctgggac gaataccgcc
     1861 gccgttggca caaaccggct ggcaccgggt cacgcaacgc ggtggcggtg gtcggcatgg
     1921 gcccggctgg cctggccgcc gggtattttc tggcgcgtgc cggccacgcg gtgacgcttt
     1981 ttgaccgcga gcaggccgct ggcggtgtgc cacgccacgt gattccgcgc tttcgcagtc
     2041 cgggcgacct ggtgcagcac gacatcaact ttgttgccga gcacggggtg aaatttgttt
     2101 atggctgcga tccgcagctc agcgtggaca agctcaaagc tcaagggttt gagtatgtgc
     2161 tgatcggcat tggcaccgac aaaaacagcg gcatccaatt gggtggcgac aaccgcaatc
     2221 tctacaaatc gctgccgttc ttgcgccgct tcaacgacgg cgaaaaactc gacctgggcc
     2281 gccatgtggc tgtggttggc gctggcaaca ccgccatgga ctgcgcccgc gctgcgctgc
     2341 gcgtgccagg tgttgaggac gtgacggtca tctatcgccg cagcttcaac gagattcccg
     2401 cctaccgtga agagtacgaa gaagcggtgg cggacggcgt gaggtttcac tttctgacca
     2461 accccgagcg ttttgatgcc gacggcactc tcacgtgtcg cgtaatggaa cttggcgcgc
     2521 ctgacggaaa aggccgccgt cgtccattgc ccacagaccg gaccattgca ctgaagatgg
     2581 atgcattgat cacagccatt ggtgagcagg ccgattgtga tgtgctcaag gcgatgggcg
     2641 ttccgctggg tgaagacggc tggcccgcag tcaaccaggc atcgagtgaa accacgcgcc
     2701 agaacgtctt tttgatcggc gatgtgaaac aagggccatc gtccatcgtt ggggctatcg
     2761 gtgatgcccg gcgtgccacc gatgagatct tgaagcgcga gaacctgaaa agccactacg
     2821 gccagaaatt ctggttgaac gccgacccca acgaaatcac ccaacgcaag ggtgccgtgg
     2881 tggtgaattt tgtcaagaaa gacgcgtttg atgccttcgt caaacaggaa ggccagcgct
     2941 gtctcgaatg caactatgtc tgcgccaaat gtgttgacgt gtgcccgaac cgcgccaaca
     3001 tctcgattgc ggtacctggc ttcaaggacc gtgcacagac gctgcacatt gatgcctatt
     3061 gcaatgaatg cggcaactgc gcccagtttt gcccatggca aggtcgcccg tacaaagaca
     3121 agatcacgat cttcagtctg ccacaagact ttgccaacag cagcaatcca ggctttctgg
     3181 tcgaggctgg caaactcaca gtgcgccagg acggcaaggt gtctgagctg accatcgaca
     3241 gtgcgggtgc ggtgaaagat gcgccgccag agctggcgga cctgtgccgc atcgccagcc
     3301 gcgtggttag ccaccacggc tacctgctca gtgcagtcga agcataagga gcaagccgcc
     3361 atgctgattc tgagaaacct gaccgcgctg caggtcgagc ctgccacagt gtggggcgat
     3421 gtccagattg ccatagacga cacgcggatt ttggaagtgg gtgccggcct cgcggcgaag
     3481 tacccgcaag cgcaagtact tgatctcaaa ggcaagctgg tgatgccagg catcgtctgt
     3541 gcgcacaatc acttctactc ggggttggcg cgcggcatca tggccaatat cttgccctgc
     3601 ccagacttca tctcgacatt gaagaatctc tggtggcgac tggaccgcgc gctcgacgag
     3661 gagtcgttgt actacagcgg cctcatctgt tccttggagg cgatcaaaag tggctgtacc
     3721 gccgtgattg atcaccacgc ctcgcccaac ttcattgccg gatcactggc aacgctgcgc
     3781 cagggtttcc tcaaagccgg gctgcgcggt atgacctgtt atgaaaccac cgaccgaaat
     3841 ggcggcgaaa aggagctgcg cgctggcgtt gacgagaacc tggcgtttgc acgtctgatc
     3901 gatgcagcca aggccaaggg tgatgagcca tatctgatgg aggcgcacct gggcgcgcac
     3961 gcgccgttca cagtatccga tgccggactg gaaatgctgg gtgacgcagt gaaaacgact
     4021 ggccgtggcc tgcacatcca tgtggccgaa gaccgctacg atgtctcaca cagccaccac
     4081 cgctacagcc aggacccgat cgagcgactg gccgcacatg gcttgattga cggcaagacg
     4141 ctgatcgggc acggcttgta cctgtccgat gccgacattg ccatcctgaa tgaccgcgat
     4201 gccttcctgg tgcacaacgc ccgctccaac atgaacaaca acgtgggcta caaccacaag
     4261 ttggccgcag ttcgcaacct ggcactgggt accgacggca tcggtgccga catgtttgag
     4321 gaactgaagt tcgcctattt caagcaccgc gacgctggcg gcagcctgtg gccagacagc
     4381 tttgcgcgct ttttgtggca tggcaacacc ttgctggagc gcaattttgg cgcacgtttt
     4441 ggccgcatcg aagcgggtta tcaggctgat ttgacgattt gcagttacga cagcccaaca
     4501 ccgctggtgg cacagaacgt gggtggtcac ctggcgtttg gcatgaactc cggcagcgtt
     4561 gacagcgtga tggtcaacgg ccaaatggtt tatcagaacc gtgcttttcc gtttgatgtg
     4621 gaacccatct atgcgcaagc acgcaaggtg gctgcgcagt tatggcagcg catggacgca
     4681 ctggtctgag cgttgctgag ttgtcctgac aacagacaaa catgatgtta agtatcaaaa
     4741 taatagctgc cagcgcttac tgcataaggg ctggaggcca atttcacgca ggcaagctgg
     4801 cagcccattt gtggcagcgc atggacgcac tggcctgagc attgctgagt tgtcctacca
     4861 acagacagat atgctgataa gagtaaaaat gatagctgcc atcgcatact gcataagggc
     4921 tggaggccaa tttgatagat gaatgccggg cggcttgcag cgccagcccg gcagcccacc
     4981 ccgatgtttt tctgaaacgt ctgctgcgcc tgccgggctg ggatgctgag tatgcgcaac
     5041 gggtattgac ttattttttc gggccgcaag gcgtgacacc acaacccgat tcgatagccg
     5101 catgaccggc attcatttcc tgtgtgctcc cggcaacagc gacttcattg cgcgttgcgt
     5161 tggtgccgat gtaccgtccg tggtcagcct ggtcggcgcc ggcggcaaga ccagcacgct
     5221 tttttggctg gctcaggcgc tcgctggctg cggtcaacgg gtcctgctga ccaccaccac
     5281 acggatgttc cggcctgagc cgggacgcgg ggcaacgctg ctgatcgagc cagagcgcgc
     5341 acaacgcctg gctgccttgc gcggtttgcc gaatgcgccg ggtatcgtgg cgctgtacag
     5401 tcaaatcgat gcaaatgagg gcaaagtgat tggcttcgaa cccgacgaaa ttgatgagct
     5461 caaggccatg gcagtggcag atgtgatcct ggtggaagcc gacggtgccc gccactgcgc
     5521 gttgaaagcg cccgcagcgc acgagccctg catcccccgt ggcagcaacg ccgtcatcgc
     5581 actgaccggt gccgcgcccc tgggttgccc ggccaatccg gccgacattc accggtggcc
     5641 gcagtttgct gccattaccg ggctgc
//
LOCUS       DAOSLL010000279         5666 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12839_length_5666_cov_4.427553, whole genome shotgun sequence.
ACCESSION   DAOSLL010000279 DAOSLL010000000
VERSION     DAOSLL010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5666
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12839_length_5666_cov_4.427553"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLB25_12500"
     CDS             complement(<1..183)
                     /locus_tag="PLB25_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42434.1"
                     /translation="MRTRFEGCCIKHCLEAASIKVYDKFSPVLRIKVTSNDISGFRHP
                     GFLRCLPQTTSEPNEIN"
     gene            986..1855
                     /gene="coxB"
                     /locus_tag="PLB25_12505"
     CDS             986..1855
                     /gene="coxB"
                     /locus_tag="PLB25_12505"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HOE42435.1"
                     /translation="MMRSLYQFGLGVALALASVATWAGYAYNFPQPVTPIAHETLHMH
                     NLVMVVILILFFLVFGLLMYSFYTHRKAAGFKAVNFTAPTTRKQWLWVLSPFALLFFI
                     DYVVFGIPALHAVLMYEDTKTDAELVVKVTGSQWLWQYEFPAEGVSFTSRLITPRAQI
                     DNSEAKGEHYLLEVDNPLVLPVGKKVRFITTSNDVIHSWWVPAFGVKRDAVPGFLREF
                     WATVEKTGTYRGQCSELCGKEHGFMPVVVNVVTQEEFAKWLASKKPATVKAPVAAVVA
                     PVQNAIVSAKATQ"
     gene            1925..3517
                     /locus_tag="PLB25_12510"
     CDS             1925..3517
                     /locus_tag="PLB25_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020656731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 1"
                     /protein_id="HOE42436.1"
                     /translation="MSHGIPHGDSHGGHYSGGFIRWLETTNHKDIGIMYLIFGFVMLF
                     IGGAMILAVRAELFQPGLQLMQPEFFNQLITLHGLVMVFGFAMPVATGLANYMLPMVI
                     GAPDMALPRLNNWGFWILPPAAIMLTLPFFLGIFGVGPGAVDTGWTLYAPLSIQSGWG
                     MDFAIFAIHMLGVSSILGSINVIVTIVNMRAPGMTLMKMPLFAHGFLMTAVLLITIMP
                     VFAGGVTMVLMDRHFDTHFFNAAGGGDPVLWQHIFWFMGHPEVYVLLLPIAGAIPHVL
                     SAFTRKPTYGYKAQVYAFWAIAGLGVIVWAHHMFTSGMSIGSQIYFMYITMAISFPLA
                     VLFFCWIASVWKGSMTFETPMLFALGFLVLFGWGGLTGLALSDAAADPQYHNAYFLVA
                     HFHYAFYPSAVMGAMAAIYYWLPKWTGHMLDESLGKWHFWVAVVGFNITFIPQFFVGL
                     AGMPRRIPDYPLMFAEWNMISSVGAFILGASHLIFVYNVIKNIRGGEKAQDKAWEGAE
                     GLEWTVPSPAPYHTFETPPEIK"
     gene            3514..3684
                     /locus_tag="PLB25_12515"
     CDS             3514..3684
                     /locus_tag="PLB25_12515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42437.1"
                     /translation="MNVLMRPSMNLSESPKPEFDPQEWSKRRGANVRLGLFVGLIVVL
                     MFLGALWKYRPL"
     gene            3681..4241
                     /locus_tag="PLB25_12520"
     CDS             3681..4241
                     /locus_tag="PLB25_12520"
                     /inference="COORDINATES: protein motif:HMM:NF003465.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HOE42438.1"
                     /translation="MTTGLTAAQERQASNQRLALRLGLVALFFVGFGFAMVPLYDAFC
                     RLTGLNGKTNSSPITADQQNTQVDLTRTVKVEFLSQAMPGVGLDFKPEQFSIRIHPGE
                     ITHVNYTVKNTSGRVFVGQAVPSVTPAAATQYFEKLECFCFTQQTFQPGESRTMPVVF
                     VVNPKLDADLGTVTLSYAFFEAPKAN"
     gene            4260..5123
                     /locus_tag="PLB25_12525"
     CDS             4260..5123
                     /locus_tag="PLB25_12525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HOE42439.1"
                     /translation="MTHLAPTAARGHYFVPAVSRYSTYLSVGVFLTALGFIFRINGTP
                     AGIWSMLIGAALITYVVIGWFGELISENLRGVYTPWEDRSYRIGMVWFIFSEVMFFAC
                     FFGALYYIRRISLPELSGYEALFTPYEKFTSAWPSAGPLGDPFSPMHAWGIPAINTML
                     LLTSGATLTWAHWGLIKGKRSQLNWGLALTVALGMTFMGFQVYEYAHAYAEMGLTLGA
                     GVYGATFYILTGFHGFHVTLGTIMLMVMLGRGLTGHFSEHNHFAFEAVAWYWHFVDVV
                     WLILFVFVYIL"
     gene            complement(5169..5375)
                     /locus_tag="PLB25_12530"
     CDS             complement(5169..5375)
                     /locus_tag="PLB25_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2909 family protein"
                     /protein_id="HOE42440.1"
                     /translation="MLAKILILLTLGIILISLFSALALLFKNDDASKRKRVVQALTIR
                     IGLSIGLFIALLASLYFGLIPGRG"
     gene            5472..>5666
                     /locus_tag="PLB25_12535"
     CDS             5472..>5666
                     /locus_tag="PLB25_12535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42441.1"
                     /translation="MPYPRFQFKLLPLLGVLAGLAMFVYLGLWQSGKGDRLHAELAQR
                     AARHQLGALPVGAVLVDAQQM"
BASE COUNT         1084 a         1515 c         1632 g         1435 t
ORIGIN      
        1 gttgatttca ttgggctccg aggtggtttg gggcaaacaa cgaaggaacc cgggatgcct
       61 aaatccagaa atgtcattgg aggtgacctt gattcgaagc accgggctga acttgtcgta
      121 taccttgata ctggccgctt ccaggcagtg cttgatgcag cacccttcaa agcgggtgcg
      181 caagcgcgag ccgatttctt ggacctttga ccgattgagg ccgcagcagc agaccaactt
      241 ctgccaccat aggccactgc gctggcgaag cgggaccgcg ctgatgctgt ccttgttttc
      301 tgctgctacc agcaattgaa gcatggcagt tgttggtaat ctcagacaat cgtgaaaacc
      361 agtccatcat gtttgctgac cagccgcacc tgaggcggca ctgctgtttt gtgacatatc
      421 agcattaagc ccttattcag caagctatgg cagctatcaa tttaataaca ctattggtcc
      481 tgtgccgtgt tactggggtc cgagcacaat tctgcgcgac agtagacgcg caaggtagcg
      541 gtgggcaatc gaatcgggat ccggcctcaa aagcgctgag tccacggtgc ggctacggcc
      601 catggaatcc agagcccatg aaacctgtgg ctcacgaggt ctgcactgcc aatgtggtca
      661 gtgtgaactg gcgacggcgg cttgtgtggc ttgggcggtc atcaggactt tgtattaaat
      721 caggctgaag gccttgttca caatccgcta gcagctataa aaatgatacc cagcgggtca
      781 tactgccgcc cgtttgggca tcgcgggcaa ttgatccggg gcagaccact atcgacacgc
      841 atcgcccagc cttttcgatc agaccagatt gccaggcggc ttcgggtttt cccccggtgt
      901 tttgcaacgg tcagcacaaa ttcgtcgtct aaaattgttt gataaatatc aaatgatcga
      961 ctatttccca tggcaaggac tgtaaatgat gcgtagtttg taccagtttg gtttaggggt
     1021 tgcgctggcg ctggcgtcgg tcgccacctg ggcgggttat gcctacaact tcccgcagcc
     1081 agtgacaccg attgcgcacg aaacattgca catgcataac ctggtgatgg tggtgatcct
     1141 gatcctgttc tttttggtgt tcgggttgct gatgtactcg ttttacaccc atcgcaaggc
     1201 agcgggcttc aaggccgtca acttcaccgc accgaccacc cgcaagcagt ggctgtgggt
     1261 gttatcgccg tttgcgctgt tgttcttcat tgactatgtg gtgtttggca taccggcgct
     1321 gcatgcggtg ctgatgtacg aagacaccaa gaccgatgcc gagctggtgg tcaaggtcac
     1381 cggcagccag tggctgtggc aatacgagtt tcctgccgaa ggggtcagtt ttaccagtcg
     1441 cctgatcact ccgcgtgcgc agatcgacaa cagcgaggcc aagggcgagc actatttgct
     1501 cgaagttgac aacccgctgg tactgccggt tggcaagaaa gtgcgtttca tcaccacctc
     1561 caacgacgtg attcacagtt ggtgggtgcc ggcctttggt gtcaagcgcg atgctgtgcc
     1621 cggtttcctg cgcgagttct gggccacggt tgaaaaaacc ggcacttacc gcggtcaatg
     1681 ttcagagttg tgtggcaaag agcatggctt catgccggtg gtggtcaatg tggtgacaca
     1741 ggaagagttt gccaaatggc tggccagcaa aaagcctgca accgtcaagg caccggtggc
     1801 ggcagtcgtc gcgccagttc aaaatgcgat cgtgtcggcc aaggccactc aatagctcgc
     1861 agcgctttaa cggcaaaccc aatttcagat tttgagcaat aaggagcagg caatggcaca
     1921 cgatatgtcg cacggcatac cgcacggtga ttcgcacggc gggcactact cgggcggatt
     1981 tattcgctgg ctggaaacca ccaaccacaa ggacattggc atcatgtacc tgatcttcgg
     2041 gtttgtgatg ctgttcatcg gtggtgccat gattctggcc gtgcgtgccg agctgttcca
     2101 gcctggcctg caattgatgc aaccggaatt ttttaaccaa ctcatcacct tgcatggcct
     2161 ggtgatggtg tttggctttg ccatgccggt ggccaccggt ctggccaact acatgttgcc
     2221 gatggtgatt ggtgcgcccg acatggcatt gccgcgcctg aacaactggg gtttctggat
     2281 tttgccgcca gccgcgatca tgctgacgct gccctttttt ctcggtattt ttggcgttgg
     2341 gcctggtgcg gtggacaccg gctggacctt gtatgcaccg ctgtcaatcc agagcggctg
     2401 gggcatggac tttgccattt tcgccatcca catgctcggt gtgtcatcca ttttgggttc
     2461 catcaatgtc attgtgacca ttgtcaacat gcgtgcgccg ggcatgacgc tgatgaaaat
     2521 gccgctgttt gcgcatggct ttttgatgac cgccgtgctg ttgatcacca tcatgccggt
     2581 gttcgccggt ggtgtcacca tggtgctgat ggatcgccac tttgacaccc atttcttcaa
     2641 cgccgctggc ggtggcgacc cggtgctgtg gcagcatatt ttctggttca tgggtcaccc
     2701 tgaggtgtat gtgctgttgc tgccaattgc cggtgccatc ccccatgtgc tgtcagcgtt
     2761 cacccgcaag cccacctatg gctacaaggc gcaggtgtat gcgttctggg ccattgccgg
     2821 cctgggtgtg attgtctggg cgcaccacat gttcaccagc ggcatgtcga tcggcagcca
     2881 gatttacttc atgtacatca ccatggcgat ctcgttcccg ttggcggtgc tgtttttttg
     2941 ctggatcgcc tcggtctgga aaggctcaat gacgtttgaa accccgatgc tgttcgcttt
     3001 gggctttctg gttctgtttg gctggggcgg cctgaccggt ctggccctgt ctgatgcggc
     3061 ggctgatccg cagtatcaca acgcctactt tttggtggcg cactttcatt acgcgtttta
     3121 cccgtctgcc gtgatgggag cgatggctgc catttattac tggttaccca agtggaccgg
     3181 ccatatgctg gacgaatccc tgggcaagtg gcatttctgg gtggcggtgg tgggttttaa
     3241 catcacattc attccgcagt tttttgttgg cctggccggc atgccgcgcc gtattccgga
     3301 ctacccgctg atgtttgccg aatggaacat gatctccagc gtgggtgcgt ttattctggg
     3361 ggcctcgcac ctgatctttg tttacaacgt catcaagaac attcgcggtg gtgaaaaggc
     3421 gcaggacaag gcctgggaag gtgctgaagg cctggagtgg acagtgccct cgccagcgcc
     3481 ctaccacacg tttgaaaccc cgccggaaat caagtgaacg tactgatgag accgtccatg
     3541 aacctgtccg agtcgcccaa gccagagttt gacccgcaag agtggagcaa gcgcaggggt
     3601 gccaatgtcc gcctggggct gtttgttgga ttgattgtgg tgctgatgtt tctgggtgcg
     3661 ctatggaaat accggcccct atgacgacag gcttgaccgc tgcgcaagag cgccaagcca
     3721 gcaaccagcg gctggcgctg cggttgggcc tggttgcact gttttttgtc ggctttggtt
     3781 ttgccatggt gccgttgtat gacgcgtttt gccggctgac cggcttgaac ggcaagacca
     3841 attcaagccc gatcacggct gaccaacaaa atacccaggt tgatttgacg cgcacggtaa
     3901 aggtcgagtt tttgagccag gccatgccag gggtcggtct tgacttcaag ccggaacagt
     3961 tttccattcg gattcatcct ggcgagatca cccatgtcaa ctacaccgtc aagaacacca
     4021 gcggcagggt gtttgtcggc caggctgttc ccagtgtcac gccggcggca gccacccagt
     4081 attttgaaaa gctggagtgt ttttgcttta cccaacagac cttccagccg ggcgagtcgc
     4141 gcaccatgcc ggtggtgttt gtggtgaacc ccaagctgga tgccgacttg ggaacggtga
     4201 cactttccta tgcatttttt gaagccccca aggcgaactg acccagaaag acccaaacaa
     4261 tgacgcacct tgcaccaacc gctgcccgtg gccattattt tgtgccggcc gtcagccgtt
     4321 actccactta cctgtcggtg ggcgtttttt tgacggcttt gggctttatc ttcaggatca
     4381 acggtacgcc agccggtatc tggagcatgc tgattggcgc tgcgctgatc acttacgtgg
     4441 tgatcggctg gtttggcgag ttgatcagcg agaacctgcg cggtgtttac acaccttggg
     4501 aggaccgctc gtaccgcatt ggcatggtct ggttcatctt ttcggaagta atgttttttg
     4561 cgtgcttctt tggcgcgctt tattacattc gtcgaatttc gctgcccgaa ctcagtggct
     4621 acgaggcttt gttcacgccc tacgagaagt tcaccagcgc ctggcctagc gctgggccgc
     4681 tgggcgaccc gttttcgccg atgcacgctt ggggtattcc ggccatcaac accatgttgc
     4741 tgctgacttc cggggccacc ttgacctggg cgcattgggg tttgatcaaa ggcaagcgca
     4801 gccagttgaa ctggggtctg gcattgacgg tggcgctggg catgaccttc atgggatttc
     4861 aggtttatga gtacgcccat gcttacgccg aaatgggctt gacgctgggt gcgggtgttt
     4921 acggtgccac tttctacatt ttgaccggct ttcacggctt tcacgtcacc ttgggcacca
     4981 tcatgctgat ggtgatgctg ggccgagggc taaccggcca cttttccgag cacaaccact
     5041 ttgcctttga agcggtggcc tggtactggc actttgtcga cgtggtgtgg ctgattttgt
     5101 ttgtgtttgt ctatattctt tagccaaatt gtccggtctt cagccctgac tttggggctg
     5161 aaacatcatt acccgcgccc cggtatcagc ccaaagtaca aactggccag cagcgctata
     5221 aacagcccga tcgacaagcc aatgcgaatt gtcagggctt gcaccacccg cttgcgtttg
     5281 cttgcgtcat cgtttttgaa cagcaaagca agcgcactga acaggctgat cagaataatg
     5341 ccaagtgtca acagaatcag tatttttgcc agcatcgcgg tctttcacaa gggtttcacg
     5401 cgattatccg ggaatgctca tcaagaggct tgacggacaa tctggcattg ccgctatttt
     5461 tttgccccgc catgccctac ccaagattcc agttcaaatt gcttcccctg ctgggtgttc
     5521 tggccggttt ggcgatgttt gtctatttgg ggctgtggca gtcgggcaaa ggcgaccggc
     5581 tgcatgccga actggcccag cgcgcggcgc gccaccagtt aggggcacta ccggtggggg
     5641 ctgtcttggt ggacgctcag cagatg
//
LOCUS       DAOSLL010000280         5625 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12941_length_5625_cov_3.306104, whole genome shotgun sequence.
ACCESSION   DAOSLL010000280 DAOSLL010000000
VERSION     DAOSLL010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12941_length_5625_cov_3.306104"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1723
                     /locus_tag="PLB25_12540"
     CDS             <1..1723
                     /locus_tag="PLB25_12540"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE42442.1"
                     /translation="DMKDVLRLLLIDDDEVDRLSVVRALKRGKVDYDIVQAVSAAEGL
                     QFAGNQRFDAILLDYRLPDQDGIEVLRLLRNGLFERTAVVMLSRHEDESLADRCLEAG
                     AQDFLLKDEVNDRRLNRALRQARQRSQIEQALQASRNELLQLSEHDLLTGLTNRRGFE
                     RALAAAVARANRDACHLAVLLIDLDDFKSVNDTAGHEVGDALLVEATRRLSATVRDTD
                     LLCRLGGDEFVVLMHSFDREDQVFLLADRLVAALQTPIVVGATPHLTTASIGVATHDP
                     EDGNAADLLRFADMAMYQAKQEGRNRSRSYSAALQESANTRSKIKHDLLSAIQNGELR
                     VFYQAQIRASDGSLGGMEALIRWQHPVLGLLAPGHFIAIAEESKAIVEIGDWVLKTAL
                     SQLNAWQLRYPQRCANLSLGINVSAVQAEANSLPLALKSALGSFCGKPGQVEIEITES
                     ALIKDTAVMASMLSVLEAQGVRLSLDDFGTGYSSLEHLKLFPISVMKIDQGFVSAIGE
                     GKKGEQLLIAIIALAKALEMKVVAEGVETQAQAEFCARHGSDLLQGYFYSRPVPADEF
                     EATFLKA"
     gene            1971..2429
                     /locus_tag="PLB25_12545"
     CDS             1971..2429
                     /locus_tag="PLB25_12545"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HOE42443.1"
                     /translation="MAIEIERKFKLKSSTVLEGLKGSYFLQAYLAKGSVLVRVRVADD
                     KAWLTIKGQPVSTLSRPEWEYEIPVADANEMARQPGAYSLEKTRYFIEHHGHMFEVDR
                     YHGGLEGLYSVEVEMDSEKAQITLPDWVGEEVTGNKAWDNESLARNGWPT"
     gene            complement(2554..3339)
                     /locus_tag="PLB25_12550"
     CDS             complement(2554..3339)
                     /locus_tag="PLB25_12550"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HOE42444.1"
                     /translation="MIVFLIDADNLSSAAWIEEACSSLEAAEGSLAVRRAYGSAENLK
                     GLSQTMLAWAIRPFVNLSLTKNTTDVALAVDAMELACQLPKPTVIAIGSGDADFVPLV
                     VRLRERGIRMICVSERTKMASEAVHAYDSVLFVGKEPGKSRNVEAAMDPVKKAPAKKA
                     TVKKASATATVETVAKKAVAKKTPAAPKSAAKAEVTLEQILQAAPALKTGQVQPLGEI
                     VKQLHNAKLIGKNAASTKLFKKFRDWFELTPEKQPNQVRYLSS"
     gene            complement(3352..3660)
                     /locus_tag="PLB25_12555"
     CDS             complement(3352..3660)
                     /locus_tag="PLB25_12555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42445.1"
                     /translation="MNDDTLDALRVQVRTPLSGDKVTIDKRLKMEILLMRSHASIVEE
                     KPGTFVCDGTYGRGVAEQKEATLLDTTTKKPIRWQPYKSHLVAVHSVLCWAVLGISVG
                     "
     gene            complement(3932..4007)
                     /locus_tag="PLB25_12560"
     tRNA            complement(3932..4007)
                     /locus_tag="PLB25_12560"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3972..3974),aa:Arg,seq:ccg)
     gene            4192..4683
                     /locus_tag="PLB25_12565"
     CDS             4192..4683
                     /locus_tag="PLB25_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOE42446.1"
                     /translation="MSDHSTATKGPKQLIVTGLFSFLIPILIIGGLVYTYMSGGQSTP
                     VSANPEKAVAQRIQKIGSVEIGDANREARTGEQVFNAQCTACHTAGVAGAPKFGDAGA
                     WGARIATGFEALLNSALKGKGAMGAQGGGDFSDYEVARAVVYMANGSGGKLAEPKKPE
                     AAK"
     gene            complement(4730..5275)
                     /locus_tag="PLB25_12570"
     CDS             complement(4730..5275)
                     /locus_tag="PLB25_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HOE42447.1"
                     /translation="MDDIVKQAMAKWPNVPHCYGWLGLDARGNWYMRDDAAQGAGAFA
                     SGAPGAKGSLLKHEKLIAFIQRNYGSDAAGQWFFQNGPQRVYVELEATPWIWRVNDQG
                     QVQAHDGQAATCLRCVMDEHGWLYLYTTVGFGLVHTQDMEQAANVIERGAWTVEEVPR
                     KELAVQYGYVTSPQATAAART"
     gene            complement(5268..>5625)
                     /locus_tag="PLB25_12575"
     CDS             complement(5268..>5625)
                     /locus_tag="PLB25_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE42448.1"
                     /translation="KREALLAARTLYDFDNVFTAPLHGFKSTEDYWARASAKPHLHTI
                     RIPTLVVNARNDPFVPAWCLPSQREVGDCVTLWQPAHGGHVGFPQGRIPGHVRSMPDA
                     VGGWLAQHTNNQTLYG"
BASE COUNT         1164 a         1549 c         1627 g         1285 t
ORIGIN      
        1 cgatatgaaa gacgttctgc gattgctgct gatcgatgac gacgaagtgg accgcttgtc
       61 ggtagtgcgc gcgctcaagc gcggcaaggt ggactacgac attgttcagg cagtcagtgc
      121 cgccgagggc ttgcagttcg ccggcaacca gcgttttgat gccattttgc tggactaccg
      181 cttgcctgat caggatggca ttgaggtgtt gcggctgcta cgcaatggtc ttttcgagcg
      241 caccgccgtg gtcatgctca gccgccacga agacgagtct cttgcggacc gctgcctaga
      301 ggctggcgcg caagattttc ttttgaaaga cgaggtcaat gaccgccggt tgaaccgcgc
      361 cttgcgccaa gcgcgccagc gcagccaaat tgaacaggcc ttgcaggcca gccgcaatga
      421 gctgctgcaa ttgtccgaac atgacctgct cacgggcttg accaatcgcc gtggtttcga
      481 gcgcgctttg gcggcggccg tcgcgcgtgc gaaccgcgat gcatgccacc tggcagtgct
      541 cttgattgac ctggatgact tcaaaagcgt taacgacact gccgggcacg aggtgggcga
      601 tgcactactt gtcgaagcca cgcggcgctt gagcgcaacc gtgcgtgata ccgacttgtt
      661 gtgccgcttg gggggtgacg agtttgttgt cctgatgcat tcatttgatc gcgaagacca
      721 ggtgttcttg ttggcggatc gtcttgtcgc tgcgcttcag acccctatcg tggtgggtgc
      781 aacgccccac ctgacaacgg ccagtattgg cgttgccacg catgacccgg aggatggcaa
      841 tgcggcggac ctgttgcgct ttgccgacat ggccatgtac caagccaagc aagaagggcg
      901 caaccgaagt cggtcgtatt ctgcggcttt gcaagaaagt gccaataccc gaagcaagat
      961 caagcatgac ttgcttagcg ccattcaaaa cggtgaatta cgggtttttt accaagcgca
     1021 gattcgcgct tccgatggca gtcttggggg catggaggct ttgatacggt ggcaacaccc
     1081 agtgcttggt ctgctggcgc cagggcactt tattgcgatt gcagaagagt ccaaggccat
     1141 tgtcgagatt ggggactggg ttctgaaaac tgcgcttagc caactcaatg cgtggcaatt
     1201 gcgctacccg cagcggtgcg ccaacctgag cttgggtatt aatgtatcgg cggtgcaggc
     1261 ggaggcgaac tctctgcccc tggccttgaa atccgcattg ggcagtttct gcggcaagcc
     1321 aggccaggtg gaaatagaga ttactgaaag cgctttaatc aaagacacgg ccgtcatggc
     1381 ctccatgttg tcggtcctgg aagcgcaagg cgttcgattg tcgctggatg actttggcac
     1441 cggttattcg tccttggagc atctcaagct gttcccaatt tccgtcatga aaattgacca
     1501 aggattcgtt tccgcgattg gtgagggaaa gaagggcgag caattgttga tcgccatcat
     1561 cgctttggcc aaggcgcttg aaatgaaggt cgtggcggag ggggtagaga cgcaagcgca
     1621 agccgagttc tgtgcgcgcc atggtagcga cttgttgcaa ggctatttct attcgcggcc
     1681 ggttcctgct gatgagtttg aggccacctt tctcaaggct tgatgccaaa tttggtcaag
     1741 tcttcagcta ttagcggtct acgatgtcgg ctatgtggtg ataagttcga gactctgtac
     1801 ttcagctttg ggtcgaaaag agacaagtac acaatcactg cgcaactacc gatgacactg
     1861 gaattaaagt cagcaccccc attgttgttg accgctcaaa gaggttccga gttccgggtc
     1921 aattcgttcc catgggaaga ttgcgcccat acaaatacga gacgttaacc atggccatag
     1981 agattgaacg caagttcaaa ctgaagtcat ccacagtact ggaaggacta aagggcagtt
     2041 actttcttca agcctatctg gccaaggggt ctgtcctcgt gcgggtcaga gttgcagatg
     2101 acaaggcgtg gctaacgatc aaggggcaac ccgtgagcac attgagtcgc ccggagtggg
     2161 agtacgaaat tcctgtcgca gacgccaatg agatggctcg tcagcccggg gcctactcat
     2221 tggagaagac ccggtatttc attgaacatc atggccacat gttcgaagtc gatcgctacc
     2281 acggcggcct agaggggctc tattccgtag aggtggaaat ggactctgaa aaggcgcaga
     2341 tcacattgcc cgattgggtt ggtgaggaag tgactggcaa caaagcctgg gacaatgaga
     2401 gcctggccag aaacggctgg ccgacttgat tgcctgcttt ttaggcatcg tgctgcaggc
     2461 caatgggcat gcgagttcta cgcacattcc ccacagttat ccccagtttc gttcacagtt
     2521 tgtgtggagg aattgactac tcaccgcccc gcctcatgac gacaggtatc gaacctgatt
     2581 gggctgcttc tcgggcgtga gttcaaacca gtctcggaat tttttgaata gtttggttga
     2641 cgcggcattt ttcccgatga gcttggcgtt atgcaactgc ttgacgattt cgccaagcgg
     2701 ctggacttga ccagttttca aagcaggtgc tgcctgcaga atttgctcca aagtgacttc
     2761 cgcttttgcg gcgctctttg gcgctgcagg tgttttcttc gctacggctt tctttgcaac
     2821 tgtttcaaca gtggctgtag cggaagcttt tttcaccgtc gctttcttgg ccggtgcctt
     2881 tttcaccgga tccatcgcgg cttccacatt tcgactcttg cccggttcct tgccgacaaa
     2941 cagaaccgag tcataggcat gcacagcctc ggacgccatc ttggtcctct cggacacaca
     3001 aatcatgcgt atgccacgct cgcgcaggcg caccaccaag ggtacaaagt cggcgtcacc
     3061 tgagccgatc gcaatgactg tcggtttggg tagctgacaa gccagttcca ttgcgtccac
     3121 cgcaagtgca acgtcagtgg tgttcttggt tagggataag ttcacaaatg ggcgtatggc
     3181 ccatgccagc atggtttgtg atagtccctt gaggttctca gcactgccat acgctctgcg
     3241 cacggccagg ctgccttccg cggcctccag actgctgcag gcctcttcga tccaggcagc
     3301 agaagaaagg ttgtctgcat caatcaggaa aactatcatg gttacatcct ttcaaccaac
     3361 ggagatgccc agcacggccc aacacagcac actgtgaacc gcgaccaggt gacttttgta
     3421 gggctgccag cgtatgggct tcttggttgt cgtatcgagc agtgtcgctt ccttttgctc
     3481 cgctacgccc cgtccatagg tgccatcgca gacaaaggtt ccgggctttt cttccacgat
     3541 gctggcatgg cttcgcatca ggagaatttc catttttaac cgtttgtcga tggtgacctt
     3601 gtccccagac agtggggttc gtacctggac ccgaagcgcg tctaacgtat cgtcgttcat
     3661 attgatgcat tgtccatgtc ttgcttaggg ctggcaggtc agcgtctgga catgggccat
     3721 gagactaata gtcggaatca gcatcatgaa gaattctgcg ggccagactt gggccatgca
     3781 tgggccacag atgggccaca gatgggccat ggaaagtaca ccggattgcc tctgacgttt
     3841 ggtcattttg aggccaagat ccggcttgac tatcggaccc cattggaaac tattgatcat
     3901 gtgggcgctg cctgtgcagt tttccctctg atggtgcggc tggcggggat cgaacccacg
     3961 acccttggct tcggaggcca atactctatc cactgagcta cagccgcaac tctaaaaaag
     4021 accaatactc acattttcgc aggaagctcg gatgacgata ggcttcggag gccaatactc
     4081 tatccactga gctacagccg cacggagaag cagcattgta gggggtttca accagtgggg
     4141 ccgctataat cgtcggctga catcaacgta cccgatttat ttgaggacac catgagcgac
     4201 cacagcaccg caaccaaagg ccccaagcaa ttgatcgtga caggattgtt ctctttcctg
     4261 attcccatcc tgatcatcgg cggtctggtg tacacctaca tgtccggtgg ccagtcgacg
     4321 ccggtttctg ccaatcccga aaaagccgtc gcccagcgca tccagaaaat cggctccgtg
     4381 gaaattggcg atgccaaccg tgaagcccgc actggcgaac aggtcttcaa tgcgcaatgt
     4441 actgcttgcc acacggctgg cgtagcgggc gcccccaagt ttggcgatgc tggtgcgtgg
     4501 ggtgcacgta ttgcgacggg ttttgaagcg ctgctgaatt ccgccctgaa gggcaaaggc
     4561 gccatgggtg cccagggtgg cggtgacttc tctgactacg aagtggcgcg cgccgtggtg
     4621 tacatggcca atggcagcgg tggcaagtta gccgagccca agaagcctga agctgccaag
     4681 taatccggag ttcactccct gaaaaagccg gccgctgatg ccggcttttt tacgtccgcg
     4741 cggccgctgt ggcctgcgga ctggtgacgt aaccgtactg cacagcgagt tccttgcggg
     4801 gaacctcctc cactgtccat gcaccgcgtt caatgacgtt ggccgcctgc tccatatcct
     4861 gggtgtgcac caggccaaac ccgacggtgg tgtacaggta cagccaaccg tgctcatcca
     4921 tgacacaacg cagacaggtt gcagcctgcc cgtcatgggc ctgcacctgg ccctggtcat
     4981 tcacccgcca gatccagggc gtggcctcca gttccacata gaccctctgc ggaccgttct
     5041 ggaagaacca ttgccctgct gcgtcactac cgtagttgcg ctggataaac gcgatgagct
     5101 tctcgtgctt gagcaggctg cccttggcgc cgggcgcacc gctggcaaac gcacctgccc
     5161 cttgcgccgc atcgtcgcgc atgtaccagt tgccacgcgc atccagtccc agccagccgt
     5221 agcagtgggg cacattgggc cacttggcca tggcctgttt gacaatgtca tccataaagc
     5281 gtctggttgt tcgtgtgctg ggcgagccag cctccgacag cgtcgggcat gctgcgcaca
     5341 tgaccgggta ttcgcccttg cgggaagccg acatggccgc catgtgcggg ttgccacagt
     5401 gtcacacagt cgcccacttc gcgctgggac ggcaggcacc aggccggcac gaagggatcg
     5461 ttgcgcgcat tgaccaccag ggtcggtatg cggatggtgt gcaggtgcgg cttggcggag
     5521 gcacgcgccc aataatcttc cgtgcttttg aagccatgca gcggcgcggt aaacacgttg
     5581 tcgaagtcat atagagtgcg tgccgcaagc agagcctccc gcttg
//
LOCUS       DAOSLL010000281         5624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_12943_length_5624_cov_4.660801, whole genome shotgun sequence.
ACCESSION   DAOSLL010000281 DAOSLL010000000
VERSION     DAOSLL010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12943_length_5624_cov_4.660801"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="PLB25_12580"
     CDS             complement(<1..125)
                     /locus_tag="PLB25_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HOE42449.1"
                     /translation="MNTPPKYQFQVQVKPRYLPDQSAPGQNLYFFAYTITIRNTG"
     gene            complement(389..802)
                     /locus_tag="PLB25_12585"
     CDS             complement(389..802)
                     /locus_tag="PLB25_12585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42450.1"
                     /translation="MAARSIAGQDDIKVKVPFARWPAVKQRATQFDTVPNQVRQICQY
                     PASLQSDQWGDCYDTNGPGRGNPPAVIVGLAHCPKAWALLRVEQAAPASLFDQGLQDQ
                     PGRQRARGQRPESYLNNSWQRIHSNGLSRFLADPH"
     gene            complement(899..1963)
                     /gene="ydiK"
                     /locus_tag="PLB25_12590"
     CDS             complement(899..1963)
                     /gene="ydiK"
                     /locus_tag="PLB25_12590"
                     /inference="COORDINATES: protein motif:HMM:NF008216.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HOE42451.1"
                     /translation="MQKPPPDLVRTTLGVLFMGGLIVAVFWVIKPFLGAIIWAAMLVV
                     ASWPLLRWFEARLWGRRALAVLVMTLLMLTLFMVPLMIAIGAIIAHADDIVNQAKSLS
                     LTQIPPPPQWLGKLPGIGEKIVVVWEQTISGGSAGIFSQLVPYGNSIAKWFVAQAGSL
                     GFIALQFLLTVVISALMYADGEAAALAMRRFGYRLASTQGENVVILASQAIRAVALGV
                     GVTALVQSLLGGIGLAMAGVPLAGLLTAVMLLLCIAQLGPGLVMFPAVAWVYWSGDTG
                     WGTFLLVWSLVVVTMDNFLRPALIRRGADLPMLLILAGVIGGLLGFGLIGIFVGPVLL
                     AVAYTLLDSWIGDASASAGQ"
     gene            2215..3268
                     /gene="holA"
                     /locus_tag="PLB25_12595"
                     /pseudo
     CDS             2215..3268
                     /gene="holA"
                     /locus_tag="PLB25_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429665.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
     gene            complement(3444..5159)
                     /locus_tag="PLB25_12600"
     CDS             complement(3444..5159)
                     /locus_tag="PLB25_12600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HOE42452.1"
                     /translation="MTDSMPTPFDYIIVGAGTAGCLLANRLSADRSRRVLLLEAGPED
                     NYHWIHIPVGYLYCIGNPRTDWLYKTEPDAGLNGRTLLYPRGKTLGGCSSINGMIYMR
                     GQARDYDQWAQLTGDDEWCWQHCLPDFMQHESHYKLDSYQRNSDKGHQAKRLEELAQF
                     HGQGGEWRVEKQRLRWDVLDAFAAAARQAGIAATDDFNSGNNEGVGYFEVNQRSGWRW
                     NTAKAFLRPACTSRPNFTLWTRAQVARLLLDRQADGSLRCAGVELVRDGEKVTLKAAV
                     EVLLSAGSIGSPQILQLSGIGPAALLQQHGIAVVHDAPGVGANLQDHLQIRAVFKVQG
                     VKTLNSQASSLWGKAQIALEYALKRSGPMSMAPSQLGAFTRSDPGQPYPNIEYHVQPL
                     SLEAFGQPLHRFAAFTASVCNLNPGSRGSVKITSPDFRVAPAIAPNYLSTEADRKVAA
                     DSLRLTRRIVAQPALAKYQPQEFKPGVQFQSDADLARLAGDIATTIFHPVGSTKMGRN
                     EDPLAVLDARLRVRGPGGLIAGLRVVDAGVMPLITSGNTNAPALMIAEKAARWILADA
                     KAHQG"
     gene            5317..>5624
                     /locus_tag="PLB25_12605"
     CDS             5317..>5624
                     /locus_tag="PLB25_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE42453.1"
                     /translation="MIAPGPTLTLQATADGRSAVLGGCWSAARLAEPANWARVTQALS
                     DCATGQADACHWVLTGLSRLDHTGAQLLWNTWGRRFPAQLSLEPGQQAMLDRVARFTK
                     P"
BASE COUNT         1073 a         1772 c         1734 g         1045 t
ORIGIN      
        1 tggccggtgt tgcggatggt gatggtgtag gcaaagaagt acagattctg gccgggcgcc
       61 gactgatccg gcaggtaacg cggcttgacc tgcacttgaa actggtactt gggtggtgtg
      121 ttcatgctgc aatgctaact gcgaaacccc gcagcccgac aggtcgaggg tgttagccag
      181 cggcacagca agcccgctgg cacccgcgct ggcggatttt cctgccggtg ccgacgggcg
      241 cactggccag caggcttcgc gggaaacacc gcagtgacta ggagtttgtg acaaaaatcg
      301 taacttctca ataatccgtc gcgaaactcg caaaatgaaa cgattccgtc attcccgcga
      361 aggcgggaat gacgggttct gccaccactc aatgaggatc tgccaaaaat cgactcaaac
      421 cattgctgtg tatgcgctgc cagctattat ttaaatagct ttcaggcctt tgacctctgg
      481 cgcgctggcg gcccggctgg tcctgcaagc cctggtcgaa caagcttgcg ggtgcagcct
      541 gctcgacacg caagagggcc catgcctttg ggcagtgtgc caggccgaca atcaccgccg
      601 ggggattgcc ccgaccaggg ccattggtgt cgtagcaatc gccccattgg tcggactgca
      661 ggcttgccgg gtactggcaa atctggcgaa cctggtttgg caccgtgtca aattgggtcg
      721 cccgctgctt gacggcgggc caacgtgcaa acggcacctt gaccttgatg tcgtcttgcc
      781 cagcgattga tcttgcagcc attgcgccct tcaaatgcca ttggcttggc agaactgcgg
      841 cccggcgggc aagcacttgt ggaccaggcc cgcttccttg gacctggtca gccgtcagtc
      901 actggccagc cgatgccgat gcgtcgccaa tccaggagtc cagcagggtg taggccaccg
      961 ccagcagcac tggcccgaca aagatgccga tcaggccaaa ccccagcaag ccaccgatca
     1021 cacctgcgag gatcagcagc atcggcaggt cggcaccgcg ccggatcagg gccgggcgca
     1081 ggaagttgtc catcgtgacc accaccagtg accagaccag caaaaatgtg ccccagccgg
     1141 tgtcgccact ccagtaaacc caggccaccg ccggaaacat gaccaggcca gggccaagct
     1201 gggcaatgca cagcagcagc atcacggcgg tcagcagccc ggccaggggc acgccagcca
     1261 tcgccagtcc gatgcccccg agcagcgact gcaccagtgc cgtgacaccc acgcccagcg
     1321 ccaccgcgcg gatcgcctgg ctggccagga tcaccacgtt ttcaccctgc gtgctggcca
     1381 gccggtagcc aaatcggcgc atcgcgagtg ccgcggcttc accatcggca tacatcagcg
     1441 ccgagatgac aaccgtcagc aagaactgca acgcgatgaa acccaggctg ccagcctgtg
     1501 cgacaaacca ttttgcaatg ctgttgccat agggaaccag ttggctgaag atgccagccg
     1561 acccgcctga gatcgtctgc tcccagacca cgacgatctt ttcgccgatg ccaggcagct
     1621 tgccaagcca ctggggtggt ggcggtatct gggtcaagga cagcgacttg gcctggttca
     1681 cgatgtcatc ggcgtgagcg atgatggcac caatggcaat catcagcggc accatgaaca
     1741 gcgtcagcat cagcaaagtc atgaccagca ccgcaagcgc acgccgtccc caaagccggg
     1801 cttcaaacca gcgcagcagc ggccaactgg ccaccaccag catggcggcc cagatgatgg
     1861 ccccgaggaa tggcttgatg acccagaaca cagccacgat caggccaccc atgaaaagca
     1921 cgcccagtgt cgtgcgaacc agatcgggcg gaggcttttg catgctgagg gactccttgg
     1981 gggtgttttt gctgaggact tgcccaggat tgtaggtagc cgccacaatt ccccagcaag
     2041 ctgggccggg ttgccgatgg cgttcacttt tgcaggaccg cctgccgccg actatgctca
     2101 ctgttggcat cgcttttcgc cgccgacccg catggccttg ttcgggcagc catgccgttt
     2161 gtgccctgcc tgggcgcttg gctgacgcca tccggggcag gctaaactgg ttcaatgcaa
     2221 ctcaacccgg cccagttatc cgcgcacctg aacaagggcc tgaaaagcct ctacaccttg
     2281 catggcgacg agcctttgct gattcaggag gcacttgatg cgattcgcac ggcagcacgc
     2341 gcgcaaggct ttaccgagcg cacctcacat accgtcagcg gggctcactt tgactggagc
     2401 gaggtgctgg ctgcctgcgg ctcgctgagc ctgtttgccg acaaacaaat tgtcgaactg
     2461 cgcatcccca gcggcaaacc cggcaaagag ggcagcaccg ccatgcagca gttggtcgat
     2521 cagtcgcgtg gcaacgatgc cacgctgacc ctggtgattt tgccgcgcct ggacaaggcg
     2581 accaaggcca gcgcctggtt tgttgcgctg gagggcggca tcacgctgga agtcagcccg
     2641 gttgagcgcg ctgccttgcc acagtggatt gcccagcgcc tggcggcgca aggccagcgc
     2701 gtgacggctg gcgaagaagg cacccgcagc ttgcaatttt ttgccgaccg ggtcgaaggc
     2761 aacttgctgg cagcacacca ggaaatccag aagctggcct tgctgcaccc ggctggtgag
     2821 ctgggttttg agcagattga gagcgccgtg ctcaacgtgg cgcgctatga cgtgttcaag
     2881 ctcagcgagg cggtgctggc cgggcaattg accagggtgc agcgcatgct tgacggtctg
     2941 caagccgaag gcgaagccga ggtgctggtg cactacaccc tgagcgagga catccgtgcc
     3001 ctcaagcgcg tcaaggatgc catcaaccgg ggtagcccgc tgccgatggc attgcgcgag
     3061 caacgcatct ggggcaacaa ggagcctgtt tgagcgtgtg ctgccgcgcc tgagcgatac
     3121 catgctggcc aaattgctgc gcagcgcgca tcaggtcgac ggggttgtca agggcatcaa
     3181 atcgcccgac tggccaggcg acccgtggca ggcgctgcac cagttggcac tgctgatgtc
     3241 gcgccagtgc atgcccaagc cccaatgaaa aaggcaccgg gggttggccg gtgccttgat
     3301 tcattttttt taacgtttgt ctggccgctg tggcctgtcc tcctcgggtc tttcaccttg
     3361 cgcaagcatt gctgcctgcc agtgatggcg ttaatgtaag ccccaaatgg gggccgggtc
     3421 atccggacat acccttttcg tatttaccct tggtgcgctt tggcatccgc caaaatccag
     3481 cgggcggcct tctcggcgat catcagcgcc ggggcgttgg tgttgccgct ggtgatcagc
     3541 ggcatgaccc cggcatcgac cacccgcagg ccggcaatca gcccgcctgg gccgcgcacc
     3601 cgcaggcgcg catccagcac cgccagcggg tcctcattgc ggcccatctt ggtgctgcca
     3661 accgggtgaa agatggtggt ggcaatgtct ccggccaggc gcgccaggtc ggcatctgac
     3721 tgaaattgca cgccgggctt gaattcctgt ggctggtatt tggccagcgc tggctgcgcg
     3781 acgatgcgcc gcgtcaggcg cagcgaatca gccgccacct tgcgatcagc ctcggtgctg
     3841 aggtaattgg gcgcgattgc cggcgccacg cgaaaatccg gcgaggtgat cttgacgctg
     3901 ccccggctgc cggggttgag gttgcagacg ctggcggtaa acgccgcaaa acggtgcagc
     3961 ggctggccaa aagcctccag gctaagcggc tgcacgtggt actcgatatt ggggtagggc
     4021 tggcctgggt cgctgcgggt gaaggcgcca agctggctcg gcgccatgct catcgggcca
     4081 ctgcgcttaa gcgcgtattc cagcgcaatc tgcgccttgc cccaaagcga gctggcttgt
     4141 gaattgagcg tcttgacccc ttgcaccttg aacaccgcgc gaatctgcaa atggtcctgc
     4201 aggttggcac cgactcccgg cgcatcatgc acgacggcaa tgccgtgctg ctgcaacagt
     4261 gctgccgggc caataccaga gagctgcaaa atctgcggcg agccaatgct gccagcgctc
     4321 aagagcactt caacagcggc tttcagcgtc actttttcgc cgtctcgcac cagttccaca
     4381 ccggcgcagc gcaaagaccc gtcggcttgc ctgtcgagca gcagccgggc cacctgggcg
     4441 cgggtccaca gggtgaagtt cggacggctg gtgcaggccg gacgcaaaaa agctttggcc
     4501 gtgttccagc gccagccgct gcgctggttc acctcaaaat agccaacccc ctcgttgttg
     4561 ccgctgttga agtcatcggt ggcggcgatg ccggcctgtc gcgcggccgc ggcaaaggca
     4621 tccagcacat cccagcgcaa acgctgcttt tctacccgcc attcgccgcc ctggccgtga
     4681 aattgcgcca gttcttcaag cctttttgcc tgatggccct tgtcactatt gcgctggtag
     4741 ctatctaatt tgtagtgact ttcgtgctgc atgaagtctg gcaaacagtg ttgccagcac
     4801 cattcatcgt cacctgtgag ctgcgcccat tggtcatagt cacgcgcctg gccgcgcatg
     4861 taaatcatgc cgttgatgct gctgcagccg cccagcgtct tgccacgcgg gtacagcagg
     4921 gtgcggccgt tcagaccggc atcgggctcg gtcttgtaca gccagtcggt gcgcgggttg
     4981 ccaatgcaat acagatagcc caccggaata tgaatccagt ggtagttgtc ctcggggcca
     5041 gcctccagca gcagcacccg cctggaccgg tcagcgctga gccggttggc cagcaggcag
     5101 ccagcggtgc cggcaccaac gatgatgtag tcaaaagggg ttggcatgga atctgtcatg
     5161 gcctcacgct ttctgaaaaa tgcgcagtgc gacgcgcctg cgtcggcaag cttgcatttt
     5221 gccgcacccg ttgaccattg atgcagaatt gatgcagcgc cattgcgagt gctgtcacgc
     5281 cgaaagcact cagcattgcc gttacgatgc gtacccatga ttgcccccgg ccctacgctg
     5341 accctgcaag ccaccgctga tggccgctct gcggtgctgg gtggctgctg gagcgcggcg
     5401 cgtctggccg agccggccaa ctgggcgcgc gtgacccagg ccctgagcga ctgcgcgact
     5461 ggccaggccg atgcctgcca ttgggttttg accggcctct cgcggcttga ccataccggc
     5521 gcacaactgt tgtggaatac ctggggccgc cggtttccgg cgcagttgtc gttggagccg
     5581 ggccagcaag ccatgctgga ccgggtggcg cgctttacca aacc
//
LOCUS       DAOSLL010000282         5594 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13014_length_5594_cov_4.005958, whole genome shotgun sequence.
ACCESSION   DAOSLL010000282 DAOSLL010000000
VERSION     DAOSLL010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13014_length_5594_cov_4.005958"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..169)
                     /locus_tag="PLB25_12610"
     CDS             complement(<1..169)
                     /locus_tag="PLB25_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42454.1"
                     /translation="MKFNSRAALTLSLALGLLGGCASMTSAPAKVADGVLVGPGGMTL
                     YTFDKDMAGSGK"
     gene            complement(263..736)
                     /gene="smpB"
                     /locus_tag="PLB25_12615"
     CDS             complement(263..736)
                     /gene="smpB"
                     /locus_tag="PLB25_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HOE42455.1"
                     /translation="MAKKPDLSSRIADNKKAAFNYFFEERFEAGIVLEGWEVKSLREG
                     KVQLTDGYVVIRNGELFIIGLQINPLGTASTHISPDRQRTKKLLMHKEEIKRLIGKIE
                     QKGYTLVPLNLHWKNGKVKCEVALAKGKAEHDKRDTIKDREGKREVERAMKSRQR"
     gene            809..1246
                     /locus_tag="PLB25_12620"
     CDS             809..1246
                     /locus_tag="PLB25_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HOE42456.1"
                     /translation="MKTVKKSVLIWYSPAEMYRLVIDVDSYPQFLPWCDKASGVPQGE
                     NGMLAEIGIAFSGIHQVFTTQNVHVPDQQVIIKLVNGPFSKLEGQWDFLTLGAEQRAC
                     KVELTLTYGFDSKLGKLISPVFDKIAGSMVDAFIKRAKQVYGE"
     gene            1236..1583
                     /locus_tag="PLB25_12625"
     CDS             1236..1583
                     /locus_tag="PLB25_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HOE42457.1"
                     /translation="MANDGTCQVTVLYAPAARLVEQVCLTLQQPCSVLQALQQSGLLA
                     RFPDIDHPAILVGVWGRRAVLGQLLRDLDRVEVYRPLLVDPKVARRERFVKQGARRAG
                     LFVKLRAGAKAGY"
     gene            complement(1614..2126)
                     /locus_tag="PLB25_12630"
     CDS             complement(1614..2126)
                     /locus_tag="PLB25_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HOE42458.1"
                     /translation="MKHIAQIAFVAASLLPLGAPAQWLWVDIDGRKVFSDRAPPSTIP
                     DKNILQRPGQPKTRPSAESADATGAAGITSAQVQASSPKVSGIDKDLAEKKKKAEQAE
                     LDKRKAEEGKANAARADNCARAKSAKAGLDSGVRLSRINAQGQREIIDDGTRAAETQR
                     LQSIIEADCK"
     gene            2225..3694
                     /gene="guaB"
                     /locus_tag="PLB25_12635"
     CDS             2225..3694
                     /gene="guaB"
                     /locus_tag="PLB25_12635"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HOE42459.1"
                     /translation="MRLIGKALTFDDVLLVPAYSQVLPKDTSLATLFTRNISLNLPLV
                     SAAMDTVTESRLAIAIAQEGGIGVVHKNMTARQQAAKVAKVKRYESGVLRDPVVITPD
                     HTVRQVMALSEQLGVSGFPVIDGGKVAGLVTGRDLRFETRLDLPVRHIMTRRDKLVTV
                     PDGTTLSEAKVLLNQHKIERLLVVNDAFELKGLITVKDITKQTSFPAAARDAQGKLRV
                     AAAVGVGEGTEERVELLARAGVDAIVVDTAHGHSMGVIDRVRWVKQNFPQVEVIGGNI
                     ATGAAALALVEAGADAVKVGIGPGSICTTRIVAGVGVPQIMAIDSVATALRGTGVPLI
                     ADGGIRFSGDIAKAIAAGASTVMMGGMFAGTEEAPGEVILFQGRSYKSYRGMGSIGAM
                     QQGSADRYFQESSTGNPNADKLVPEGIEGRVPYKGSMVAIIFQMAGGLRASMGYCGCA
                     SIEEMQNKAEFVEITTAGIRESHVHDVQITKEAPNYRAE"
     gene            3780..5423
                     /gene="guaA"
                     /locus_tag="PLB25_12640"
     CDS             3780..5423
                     /gene="guaA"
                     /locus_tag="PLB25_12640"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HOE42460.1"
                     /translation="MHQKILILDFGSQVTQLIARRIREAHVFCEVHPCDVSDDWLRAY
                     ARDGMLKGIILSGSHASTYEEHELRASQVVYQLGIPVLGICYGMFTMAVQLGGLVAPS
                     NKREFGHADVRAHAHTALLEGIQDYATSMGHGMLQVWMSHGDKVTELPPGFKLMASTD
                     SCPIAGMADEERRFYGLQFHPEVTHTKRGAAILERFVLDICGARADWIMGDYISEAVA
                     KIRAQVGCEGVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGDMVMEMF
                     VGKLHAKVIRVDAAGQFLGQLAGVSDPESKRKIIGREFVEVFKAQALALTAQGAIDNV
                     ARQGPAVKAATWLAQGTIYPDVIESGGAKNKKAVVIKSHHNVGGLPEQLGLKLLEPLR
                     ELFKDEVRELGVALGLPHNMVYRHPFPGPGLGVRILGEVKKEYADLLRRADAIFIEEL
                     HNFVDESSGKNWYDLTSQAFTVFLPVKSVGVMGDGRTYDYVVALRAVQTSDFMTADWA
                     ELPYALLKKVSSRIINEVRGINRVTYDISSKPPATIEWE"
BASE COUNT         1130 a         1575 c         1673 g         1216 t
ORIGIN      
        1 atttgccgct gccggccata tccttgtcga atgtgtacag ggtcatgccg cctggcccaa
       61 ccagcacccc atcggcaacc ttggccggtg ccgacgtcat ggaggcacat cccccaagaa
      121 gaccaagagc cagggacagc gtcagggcgg cgcgtgaatt gaatttcatg atgaactccg
      181 gagttgatga acgcaaacag gcgtctgcag ctccataaac accggttggc cgtggtttat
      241 tccatggctc agaaaatatt tctcaacgtt gcctggattt catggcgcgc tctacctcgc
      301 gcttgccttc acggtccttg atggtgtcgc gtttgtcgtg ttcggccttg cccttggcca
      361 gggccacttc acatttgacc ttgccgtttt tccagtgcag gttgagcggc accagggtgt
      421 agcccttttg ctcgatcttg ccaatcagtc gtttgatctc ttccttgtgc atcagcagct
      481 ttttggtgcg ctgcctgtcg gggctgatgt gggtcgatgc ggtgcccagc gggttgatct
      541 gcagcccgat gatgaagagc tcgccattgc gaatcaccac atagccgtcg gtcaactgca
      601 ccttgccttc acgcagcgat ttgacctccc agccttcgag cacaatgccg gcttcaaagc
      661 gctcctcaaa aaaataattg aaggcggctt ttttgttgtc ggcaatgcgg ctggataaat
      721 cgggtttttt ggccatgggt tgagcttaaa tgactgactg aacaatgcga accaggcttg
      781 actacaatcc cgcgcaatca tccattctat gaaaactgtc aaaaaatccg tcctcatctg
      841 gtacagcccg gccgaaatgt accgcttggt catcgatgtg gactcatacc cacagtttct
      901 gccatggtgc gacaaggcga gcggggtgcc gcaaggagaa aacggcatgc ttgccgaaat
      961 tggcatcgcg ttcagcggca ttcaccaggt tttcaccacc cagaatgtgc atgtgcctga
     1021 ccagcaggtg atcatcaaac tggtcaatgg cccattttcc aagcttgaag ggcaatggga
     1081 tttcctgacg ctgggtgccg aacaacgcgc ctgcaaagtc gaattgaccc tgacctatgg
     1141 ctttgacagc aagctgggca aactcatcag cccggttttt gacaagattg ccggcagcat
     1201 ggtggacgct tttatcaagc gcgccaagca ggtttatggc gaatgatggt acctgtcagg
     1261 taacggtact gtatgcaccg gcagcgcgcc tggtggagca agtttgcctg accctgcagc
     1321 agccttgcag tgtgctgcag gcactacagc aaagcggctt gctggcgcgt tttcccgaca
     1381 ttgaccaccc ggccatcctg gtgggtgtct ggggtcgtcg cgctgtactt gggcaactgc
     1441 tgcgtgactt ggaccgggtc gaggtttacc ggccattgtt ggtggacccc aaggttgcgc
     1501 gacgcgagcg ctttgtcaaa caaggggcgc gccgcgctgg cctgtttgtc aagctacgcg
     1561 caggagccaa ggccggttac tgagattgcc ggagttccat tggccgcttg tcgctatttg
     1621 caatctgcct cgatgatgct ctgcagccgc tgtgtttcgg cagcacgggt gccatcgtcg
     1681 atgatttccc gctgtccctg agcgttgatc cggctcaatc gcacgccgga atcaagcccg
     1741 gccttggcag acttggcccg cgcacaattg tcggcccgcg ccgcgttggc tttgccttcc
     1801 tccgccttgc gtttgtccaa ctcggcttgt tcggccttct ttttcttctc ggccaaatcc
     1861 ttatcgatgc ccgagacctt gggcgaactc gcctgcacct gggctgatgt gatgcccgct
     1921 gcgccagtcg cgtcagcgga ttcggcactc gggcgggttt tgggttgacc agggcgttgg
     1981 agaatatttt tatcaggaat tgtgctcgga ggcgcacggt cactgaagac cttgcgaccg
     2041 tcaatgtcaa cccacaacca ttgggccgga gctcccagcg gcagcaggct ggcagcaaca
     2101 aaagcaatct gtgcaatgtg tttcatgggg gccgagttta tgccacagcg aacgattcag
     2161 cccaacatgt cgcgtccagg cacccaatcg ccccagatac aatcacaaat ttggagcttg
     2221 caacatgcgg ttgattggaa aagcgctcac cttcgacgat gttttgttgg taccagccta
     2281 ctcccaggtc ctgcccaagg acacttctct ggctaccctg tttacccgca acatctccct
     2341 gaacctgccg ctggtgtccg ccgccatgga caccgtgacc gagtcacgac tggccatcgc
     2401 catcgcccag gaaggcggca ttggcgtggt gcacaagaat atgacggccc gccagcaggc
     2461 ggccaaagtg gcgaaggtca aacgttacga gtcgggtgtg ctgcgtgacc cggtggtcat
     2521 tacgcctgat cacacggtgc gtcaggtgat ggcgctgtca gagcagttgg gtgtatctgg
     2581 ttttccggtg attgacggcg gtaaggtggc cggcctggtg accgggcgtg acctgcgctt
     2641 tgaaacccgg cttgacctgc cggtgaggca catcatgacc cggcgcgaca agctggttac
     2701 cgtgccggat ggcacaaccc tgtccgaggc caaggtgctg ctcaatcagc acaagatcga
     2761 gcgcttgctg gtggtcaatg acgcgtttga actcaagggc ctgatcacgg tcaaggacat
     2821 taccaaacaa accagcttcc ctgctgcggc gcgtgacgcg caaggcaagc tgcgggtggc
     2881 cgccgccgtc ggggtgggtg aaggcaccga ggaacgggtg gaactgctgg cacgcgctgg
     2941 cgttgatgcg atcgtggtgg acaccgcgca tggccacagc atgggcgtga ttgaccgggt
     3001 gcgctgggtc aagcaaaact tcccgcaggt tgaggtcatt ggtggcaaca ttgccaccgg
     3061 tgctgcggcg ctggcgctgg tcgaagccgg tgctgacgca gtcaaggttg gcattggccc
     3121 aggctcgatt tgcaccaccc gcatcgtcgc cggtgtaggt gtgccgcaaa tcatggcaat
     3181 tgacagtgtc gccaccgccc tcaggggcac cggtgtgccg ctgattgccg atggtggcat
     3241 ccgtttcagc ggtgacattg ccaaggcaat cgccgccggt gccagcaccg tgatgatggg
     3301 tggcatgttt gccggcaccg aagaggcacc aggcgaagtc attttgttcc agggccgcag
     3361 ctacaagagc taccgtggca tgggcagcat tggcgcaatg cagcagggca gtgctgaccg
     3421 atattttcag gaatcgagca ccggcaatcc gaacgctgac aagttggtgc ccgaaggcat
     3481 agagggccgt gtgccctaca agggctcaat ggtcgccatc attttccaga tggcaggtgg
     3541 cctgcgtgcc agcatgggct actgtggctg cgccagcatc gaagagatgc aaaacaaggc
     3601 cgagtttgtc gagatcacca ccgccggcat ccgcgagagc catgtgcacg acgtgcagat
     3661 caccaaagaa gcgccgaact accgcgctga ataattgatc gtcggctgtc ggctgctgaa
     3721 tatgtgtgac caccgctgtg gcaaaaaatg aacttggctc agctttcaac tggttgaata
     3781 tgcatcaaaa aatcctcatc ctcgacttcg gctcccaagt cacccagttg atcgccaggc
     3841 gcatccgcga agcccatgtg ttttgcgaag tgcatccgtg cgacgtgtca gacgactggc
     3901 tgcgtgccta tgccagagac ggcatgctca aaggcatcat tctgtcgggc agccatgcca
     3961 gcacatatga agagcacgaa ctgcgggcat cccaggtggt ctatcaactc ggcattccgg
     4021 tgctgggtat ctgttatggc atgttcacca tggcggtgca actcggcggg ctggttgcac
     4081 cgagcaacaa acgtgaattt ggtcacgccg atgtgcgcgc ccacgcgcac accgcattgc
     4141 ttgaaggcat tcaggattac gccacgtcga tgggccacgg catgttgcag gtctggatga
     4201 gccatggcga caaggtcacc gagctgccac ctggctttaa gctgatggcc agcaccgaca
     4261 gttgcccgat tgccggcatg gccgacgaag aacgccggtt ttacgggctg cagtttcacc
     4321 cggaggttac ccacaccaaa cgcggcgcgg cgattctgga gcgctttgtg ctggacatct
     4381 gcggcgcgcg ggccgactgg atcatgggcg actacatcag cgaagcggtg gcaaagatcc
     4441 gcgctcaggt cggctgcgag ggagtgattc tgggcctgtc cggcggggtt gattcttcgg
     4501 tggctgcggc cctgattcac cgtgccattg gcgatcagct cacctgtgtg tttgtcgatc
     4561 atggcctgct gcgcctgaat gaaggcgaca tggtgatgga gatgtttgtc ggcaaattgc
     4621 acgccaaagt catccgggtc gatgctgctg gtcagttttt ggggcagctc gcgggtgtca
     4681 gtgacccgga atccaagcgc aagatcatcg ggcgtgaatt tgtagaggtg tttaaggcgc
     4741 aagcccttgc cctgactgcg cagggagcta ttgataacgt agcaagacaa ggtcctgccg
     4801 tgaaggctgc aacgtggctg gcgcaaggca ccatttaccc ggatgtgatc gaatcgggtg
     4861 gtgccaaaaa caaaaaggcg gtggtcatca agagccacca caatgtcggt ggtctgcccg
     4921 agcagctcgg cctgaaattg ctggagccgc tgcgcgagct gttcaaggac gaggtacgcg
     4981 aactcggtgt ggcacttggc ttgccgcaca acatggttta tcgccacccg tttcccggcc
     5041 ctggcctggg cgtgcgtatt ctgggtgaag tcaagaagga atacgccgac ctgctgcgcc
     5101 gggcggatgc catcttcatc gaagaattgc acaactttgt cgacgaaagc agtggcaaga
     5161 attggtacga cctgaccagc caggccttca ccgtgttttt gccggtcaag agtgtcgggg
     5221 tcatgggcga cggtcgcacc tacgattacg tggtggcact gcgcgcggtg caaaccagcg
     5281 acttcatgac cgccgactgg gccgagttgc cctatgcgct gctcaagaag gtgtcaagcc
     5341 gcatcatcaa cgaagtgcgt ggcatcaacc gcgtcaccta cgacatctca tccaaaccgc
     5401 cagccacgat tgagtgggag taattcataa ctggacagtg ccaagttctc cttgttatgc
     5461 ggggagaact tggcactgtc cagaaataaa ctgaaaaatc gtcttgacac ttgggtccaa
     5521 tttaatcggc atcacttcac aactggacag tgcgtagttc cccccttcgt gttcaggccg
     5581 ccattggctg cgca
//
LOCUS       DAOSLL010000283         5585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13046_length_5585_cov_4.962206, whole genome shotgun sequence.
ACCESSION   DAOSLL010000283 DAOSLL010000000
VERSION     DAOSLL010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13046_length_5585_cov_4.962206"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="PLB25_12645"
     CDS             complement(<1..218)
                     /locus_tag="PLB25_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42461.1"
                     /translation="MEYALAAIILDLSLGGGRCYWGLNPFAFWCCGFRPTNPRRLRFT
                     RARPTADSRTEDVVRNNFPYLHRRIWAH"
     gene            complement(327..1433)
                     /gene="selD"
                     /locus_tag="PLB25_12650"
     CDS             complement(327..1433)
                     /gene="selD"
                     /locus_tag="PLB25_12650"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HOE42462.1"
                     /translation="MATLAQNPESSPIATTLATPVQPRLTSLSHGGGCGCKIAPGVLS
                     NILKGTSAMPMPPELLVGIETADDAAVYQLNDEQALIATTDFFMPIVDDPFDFGRIAA
                     TNAISDVYAMGGTPIMALALVGMPIAVLSIDTIGKILEGGASVCRAAGIPIAGGHTID
                     SVEPIYGLVALGLVHPSRVKRNASAQVGDRLVLGKPIGIGILSAALKKDQLDAAGYAQ
                     MMAVTTQLNTPGPVLAALPGVHAMTDVTGFALAGHALEIARGAASTLAIDWARVPLLR
                     GARELALAGNITGASGRNWAAYGHEVCLPGGFAAVDQALLSDPQTSGGLLVTCSPDAV
                     EAVLAVFHKMGFDAAADIGEVTAAAANGCALLLR"
     gene            1530..2486
                     /locus_tag="PLB25_12655"
     CDS             1530..2486
                     /locus_tag="PLB25_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOE42463.1"
                     /translation="MTDSQTPTPLASLQAKPRYLSAPLAHIGAWTRYFRSAEIPVLAR
                     TAQALEALRAIEDDVDANMLTSVIQADPLMTLKLLAHVATLRHPSNMTETESVTSSLV
                     LMGIAPFFRKFGQQATIENWLADQPEALQGVQEQLKRAERAGQFALGFAIHRGNTDAA
                     IVHLAAFLHNFADLLVLLHAPALAVKIQHAQSFDAALRSSIIQKEVLGVEMNDLSLAL
                     MKLWHLPELLVRSSDERHAEHANVQWVMLAVRLARHSAAGWDNAALPDDVADAARLFN
                     APARVVLAFLHKIDHPVLTATLLTDRQPAQAPPTAPDQPGQG"
     gene            2491..3177
                     /locus_tag="PLB25_12660"
     CDS             2491..3177
                     /locus_tag="PLB25_12660"
                     /inference="COORDINATES: protein motif:HMM:NF024790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HOE42464.1"
                     /translation="MKSRTLLFLSGAAIALIWQLPYGRLMLYPLSLLATYAHEMGHGL
                     SALLVGENFDALLLHADGSGMAAWHGNPGRVATALIAAGGLVGPTLAGISLLLLARSA
                     RFARAVLVLLAALLVISVGLWSRNAFGVGFLLALAVALGLAAKVLPDVAAAFLLHLIA
                     LTLCLSWFTDLNYMFSAQAVVGGVVHPSDSAAIAEVLWFPYWFWGGLIAAFSLAMALL
                     GIRLASRDSV"
     gene            3393..4094
                     /locus_tag="PLB25_12665"
     CDS             3393..4094
                     /locus_tag="PLB25_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE42465.1"
                     /translation="MKGMLDFLEKAGLVTRDTPQPQAVPEIEIELTPGTPPPPPSPPP
                     PPPPLAEVGTGAPVANAGVALNLGDIYARAGIAPALYPAERLLRLVDGLSAMDAATRL
                     MAINAMDAADDSWTMADPLADAAAKVAALGSHGESLKLKLQQLELETQSVLDGVLARQ
                     EKVVGDIRKQIAELEALVARELSRSAQETAEYQASLQAARDQTSRELAELTQVSLRLQ
                     SLSSQFGTPSTPAQD"
     gene            4098..4622
                     /locus_tag="PLB25_12670"
     CDS             4098..4622
                     /locus_tag="PLB25_12670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42466.1"
                     /translation="MASLTPLSKGLIGLAVLGGMASAAWHLYLKDRLGSTATQPAPAA
                     VSTPMATDRPTAPATVTPTAPTTQAMPTTPTVAAPATQPVKAPNDGHLQASGRSAAEN
                     AEAGRKALAAGDFAQARANLEQAVQDGDAGAACLLGEMTLKGQGGIAASQDKAAKLFQ
                     LAQSRNTICFTAGQ"
     gene            4714..>5585
                     /locus_tag="PLB25_12675"
     CDS             4714..>5585
                     /locus_tag="PLB25_12675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE42467.1"
                     /translation="MKIRSLALQTALSCAILVTGSAHAQGFVFGTAQPAQPAQPAKPQ
                     AAAQVPAGSRTSKVETAQRLLARMGLLREAPSGTLTPATQEAIRAFTSQNGLPATNQV
                     TDGLLNAIRRVIWTTQNWSSGNYKGREKLVDAAGLREAQILLGKLGFNAGPLDGTFGP
                     QTQVATEAFQESQAVTVDGLITATVLMNLRRAVNGVGTSARSTVRLLNWPDYIEPSVL
                     QDFEKETGIRVIYDIFSGNDDLQTRLAAGGTPYDVVFPTANAVPAMAAKGLLGKLDKA
                     SLKNIGNIDPRVDAT"
BASE COUNT         1092 a         1808 c         1659 g         1026 t
ORIGIN      
        1 ctgtgcgccc aaatccgccg gtgcaaatag gggaagttgt ttctgaccac atcctcagtc
       61 ctggaatctg cagttggacg cgcccgagtg aatcgcaggc ggcgcggatt ggtggggcga
      121 aagccgcagc accaaaatgc aaatgggttc agaccccaat agcaccggcc accacccaag
      181 gataaatcaa gaataatagc tgccagcgca tattccacgg gggctagaag ctaatttctc
      241 ataaaactca agacaatgtc aagactctgt gcaaccgcat ttcggcagcc ttggcaagat
      301 gaagtgggaa cccttggcca atgacctcag cgcaacaaca gcgcgcatcc attcgccgcc
      361 gccgccgtga cttcaccaat gtcggctgca gcgtcaaacc ccatcttgtg aaacaccgcc
      421 agcaccgcct caaccgcgtc gggcgagcag gtcaccaaca gcccgccgct ggtttgcggg
      481 tctgacagca aggcctgatc aaccgctgca aaaccgcctg gcaaacacac ctcgtgccca
      541 tacgcagccc agttgcgccc tgatgcgccg gtgatgttgc ccgccaatgc caattcgcgt
      601 gcgccacgca gcagcggcac cctggcccag tcaattgcca gggtactggc cgcaccacgg
      661 gcaatttcca gcgcatggcc ggccagtgcg aaaccggtca catcggtcat ggcgtgcacg
      721 cctggcaacg cggccagcac cggcccgggg gtgttgagct gggtggttac cgccatcatc
      781 tgtgcatacc cggcggcatc aagctggtct ttcttcagcg ctgctgacaa gatgccgatg
      841 ccaatcggtt tgcccagcac cagccggtcg ccaacctgcg cgctggcgtt gcgcttgacc
      901 cggcttgggt gcaccaggcc caacgccacc agcccgtaaa tcggctcgac cgaatcaatg
      961 gtgtggccac cagcgatggg aatgcccgcc gcccggcaaa cgctggcccc gccttccaga
     1021 atcttgccaa tggtgtcaat tgacagcacg gctatcggca tgcccaccag cgccagcgcc
     1081 atgatcggcg tgccgcccat ggcgtacacg tcgctgatgg cgttggttgc ggcaatgcgg
     1141 ccaaagtcaa acgggtcatc gacgatgggc atgaaaaaat cggtcgtggc aatcagcgcc
     1201 tgttcgtcat tgagttggta gaccgccgcg tcgtcagcgg tttcaatgcc caccagcagt
     1261 tccggcggca ttggcatggc gctggtgcct ttcagaatat tcgacaacac accaggggca
     1321 atcttgcagc cacagccgcc gccgtgcgac aggctggtca ggcgcggttg cacgggtgtt
     1381 gccagcgtgg tcgcaatcgg tgaactttcg gggttttgtg ctagtgttgc catttggatt
     1441 tggtaaaagc tgtcgatacc cgattatccg gcttgacctg cccagccacc gcccgcatac
     1501 ttatttgcac ccgattgacc tagtgaccca tgaccgacag tcagacccca accccgctgg
     1561 catcactgca ggcaaaaccg cgttacctgt cagcgccgct cgcgcacatt ggtgcctgga
     1621 cgcgctattt ccgcagcgcc gaaataccgg tgctggccag aaccgcgcag gccctggaag
     1681 cattgcgcgc catcgaagac gatgtggatg ccaacatgct cacctcggtc atccaggccg
     1741 acccgctgat gacactcaaa ctgctggccc atgtggccac gctgcgccac cccagcaaca
     1801 tgacagagac cgagtcggtc accagctcac tggtgctgat gggcattgcc ccgtttttcc
     1861 gcaagtttgg ccagcaagcg actatcgaga actggctggc agaccagccc gaggcattgc
     1921 aaggtgtgca agagcaactc aagcgggccg agcgggccgg ccagtttgcg ctgggctttg
     1981 caattcaccg tggcaatacc gatgccgcaa tcgtccacct ggccgcgttt ctgcacaact
     2041 ttgccgactt gctggtgctg ctgcatgcgc cggcactggc cgtcaaaatt cagcacgcac
     2101 agagctttga tgccgcactg cgctcgagca tcattcaaaa agaggtgctg ggggttgaga
     2161 tgaacgatct gagcctggcc ctgatgaaac tctggcattt gcctgaactg ctggtgcgca
     2221 gcagcgacga gcgccatgcc gaacacgcca atgtgcagtg ggtgatgctg gcggtgcggc
     2281 tggccagaca ctctgccgcg ggttgggaca acgccgcatt gcctgacgac gtggccgacg
     2341 ccgcccggct gttcaacgcc ccggccaggg tggtactggc gtttctgcac aaaattgacc
     2401 acccggtgct aacggccact ttgctcaccg accggcagcc ggcccaagcg ccgccaactg
     2461 ccccggatca gcctggtcag ggctgaggcc atgaaatcgc gcacattgct gttcctgtct
     2521 ggcgctgcga ttgccctgat ctggcagttg ccctacggca gattgatgct gtatccgctg
     2581 agcctgctgg ccacttacgc ccatgaaatg ggtcacggcc tgagtgcatt gctggtcggg
     2641 gaaaactttg atgcgttgct gctgcacgcg gacgggtctg gcatggcagc ctggcacggc
     2701 aaccctgggc gcgtcgccac cgccctgatt gcggctggcg gactggtcgg tccaacgctg
     2761 gctggcatca gcctgctgtt gctggcacgc tcggcacgct ttgcccgtgc cgtgctggtg
     2821 ctgctggcag ccctgctggt gatttcggtc ggcttgtggt cgcgcaatgc cttcggtgtc
     2881 ggctttttgc tggccttggc ggtggccctg gggctggccg ccaaggtgct gcctgacgtg
     2941 gctgctgcgt ttttgctgca tttgattgca ctcacgctgt gtttgtcctg gttcaccgac
     3001 ctgaattaca tgttttccgc acaggcggta gtgggcggtg tcgtccaccc gtcagacagc
     3061 gctgcgattg ccgaggtgct gtggtttccc tactggtttt ggggtggtct gattgccgct
     3121 ttttcgctcg ccatggcgct gctggggatt cgcctggcaa gccgcgattc agtgtaaaaa
     3181 caagctgtag cccccgtctg acaagcgctg gcagctatac tttttgattt tatcggggaa
     3241 ctttggcagt tcgcttgaag gtatccgggc cactgcgacc ggcaagcgat gccggccgtg
     3301 cctggggcga cccatattgg cattaaactc attgcggcgc tactggccga cgcagtaggc
     3361 gtggcacatc aaaaccaact tggaaacacg caatgaaggg catgctggac tttctggaaa
     3421 aagcaggact ggttaccagg gacacgccgc aaccgcaggc cgtgcctgaa atcgagatcg
     3481 aactgacacc gggcacacca cccccacccc catccccacc gccaccgcca ccgccgcttg
     3541 cagaagttgg caccggggca ccggtggcaa atgccggtgt ggcactgaac ctgggtgaca
     3601 tctatgcccg ggccggcatt gccccggccc tgtacccggc cgagcggctg ttgcgcctgg
     3661 tcgatggtct gagcgccatg gacgcggcaa cccgccttat ggccatcaac gcgatggatg
     3721 ccgccgatga ttcctggacc atggccgacc cgctggccga tgcggcagcc aaggtggcgg
     3781 ctcttgggtc gcatggcgag agcctcaagc tcaagctgca gcagctcgaa cttgaaaccc
     3841 agagcgtctt ggacggtgtc ctggcacgcc aggaaaaggt ggtcggcgac attcgcaaac
     3901 agattgccga gctggaagcg ctggtggcac gcgaactgtc acgcagcgcg caagaaaccg
     3961 ccgagtacca ggccagcctg caggctgcgc gcgaccagac cagccgggag ctggctgagt
     4021 tgacacaggt cagcttacgg ttgcaaagcc tgtcgagcca gtttggcacc ccctcaacgc
     4081 ctgcacagga ctgattcatg gccagtttga cacccctctc caaaggcctt atcggcctgg
     4141 ccgtgctggg cggcatggca tctgccgcct ggcatctgta tttgaaagat cgcctgggca
     4201 gcactgccac gcagcctgcg cccgctgccg tcagcacgcc tatggcaaca gatcgcccga
     4261 ccgcaccggc caccgtcacg cccacagcgc ccacaacgca agcgatgcca acaaccccga
     4321 ccgtcgcagc gcctgccacc cagcccgtca aagcgccgaa cgacggtcat ttgcaagcct
     4381 cagggcggtc tgccgctgaa aatgccgaag ccggacgcaa ggcgctggcc gctggcgact
     4441 ttgcccaggc ccgtgcaaac ctggagcaag cggtacagga cggcgacgcg ggcgcagcct
     4501 gcctgcttgg cgaaatgacg ctcaagggcc agggtggcat tgcggccagc caggacaagg
     4561 cggccaaact gttccagttg gcccaatctc gcaacaccat ttgcttcacg gctggacagt
     4621 gagcaggctg gtcattgaca aaacggctag tcaccactcc aaccgtcgcg gcaagcccgc
     4681 cgcttcagac ccatttttga ccaggatcac cccatgaaaa tacgctccct tgcactgcag
     4741 accgccctga gttgcgccat cctggtgact ggttcggcac atgcccaggg ctttgttttt
     4801 ggcacggccc aaccggcaca accggcccag ccagccaagc cgcaagccgc tgcccaggtg
     4861 ccggcaggca gccgtacttc caaggtcgaa accgcccagc gcctgctggc ccgcatgggg
     4921 ctgctgcgtg aggcccccag cggcacgctg acaccggcca cgcaggaagc cattcgcgcc
     4981 ttcaccagcc aaaatggtct gcccgccacc aatcaggtca cagatggctt gctcaacgcg
     5041 attcgccgcg tcatctggac cacgcaaaac tggtccagcg gcaactacaa ggggcgcgaa
     5101 aaactggtcg atgcggccgg actgcgtgaa gcccagattt tgctgggcaa gctgggtttc
     5161 aatgcggggc cgctggacgg cacttttggc ccgcaaaccc aggtcgccac cgaagcgttc
     5221 caggagtccc aggcggtcac cgtcgatggt ctgatcaccg ccaccgtgtt gatgaacctg
     5281 cgccgggctg tcaatggcgt gggcaccagc gccaggagta cggtgcgcct gctcaactgg
     5341 cccgactaca ttgagcccag tgtgctgcaa gacttcgaga aagaaaccgg catccgcgtg
     5401 atctacgaca ttttttccgg caatgatgat ctgcagacca ggctggctgc gggtggcaca
     5461 ccctatgacg tggtgtttcc caccgccaac gcagtgcccg ccatggccgc caaaggtctg
     5521 cttggcaagc tcgacaaggc cagcctgaaa aacattggca acatcgaccc gcgcgtcgat
     5581 gccac
//
LOCUS       DAOSLL010000284         5583 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13052_length_5583_cov_4.375724, whole genome shotgun sequence.
ACCESSION   DAOSLL010000284 DAOSLL010000000
VERSION     DAOSLL010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5583
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13052_length_5583_cov_4.375724"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            223..765
                     /locus_tag="PLB25_12680"
     CDS             223..765
                     /locus_tag="PLB25_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HOE42468.1"
                     /translation="MRTSGLICCLLLAFVAHADPAESPDELGSFLVNNSVLLQIDQVR
                     QSVATKASNLVVNALGFLGVPYRRGGSSAEGGFDCSGFVKSTYEQTLGLILPRKAEQQ
                     AAATQQIERTELQPGDLVFFNTMRRAFSHVGIYIGEGKFVHSPKPGSEVRIDNMTQSY
                     WSRRFDGARRVQALATDANR"
     gene            1297..3318
                     /locus_tag="PLB25_12685"
     CDS             1297..3318
                     /locus_tag="PLB25_12685"
                     /inference="COORDINATES: protein motif:HMM:NF024295.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 44 protein"
                     /protein_id="HOE42469.1"
                     /translation="MNWHLICNRHVFSFAALAFSAPMLAAATGPALSIDAAAGQRAIN
                     PDIYGVNELSDEALATELRLPVRRWGGNTTSRYNWQIDTYNTGLDWYFENIPLANANV
                     AALPSGSSTDRFVEQNQRTGSRTLMTVPTLGWVAKRRTDGHPFDCGFKVSRYGAQQST
                     DYWDPDCGNGLTMGGSNITANNATDTSVVSNPAFIAAWVQHLVDKYGTAANGGVSYYA
                     FDNEPMLWHSTHRDVHPQPGTYDEMRDRALAYGAAIKAVDPSAKTLGPVEDGWCRYLY
                     SAADDCGPGADYAAHGSVQYVPWYLQQMKSYEQQHGLRVLDYLDLHNYPAASGVTLSP
                     AGNAATQALRLRSTRSLWDASYIDESWISDTAPGGVAVRLIPRMKQWVADHYPGTRLA
                     ISEYNWGAPESINGALAQADVLGIFGREGLDLATLWGEVFSAQPLAFAFRMYRNYDGA
                     GHGFGDTSVQAISADQSALAVYAARRSADGALTIMILNKSATDLTTAVSLAGFNPKAS
                     ARVFRYSPANLGAIESRVAQTVSASGFSASFAANSVTLLELPPQDPAVSLSTTSLAFG
                     NQTAGTGSTARTITLTNTGGAALSLAISAPATSAFSVTHDCPASLAAGAGCSLTARFT
                     PTTAGSVTDAIVLTTNAAGSPHLLTLTGQGLPSAVKRVDLTPILMLLLE"
     gene            4283..5452
                     /locus_tag="PLB25_12690"
     CDS             4283..5452
                     /locus_tag="PLB25_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE42470.1"
                     /translation="MTDMKKRSLIRVGALSAVAAAAALVGCGKKEEPAPAPVAAAPAS
                     APASAPAKPAPLKIGFAYVGPVGDGGWTFAHDNGRKMLEKEFGDKIVTSFVENVPESA
                     DAERVIRDMAGQGNKLIFGTTFGYMEPMLKVAGDLKDVKFEHATGYKQADNMRTYDSR
                     TYEGAYMAGVIAGKMTKSNTLGVVASIPIPEVIRNINSFTLGAQSSNPKVKTKVVWVN
                     GWFDPPKETEAATSLINGGADVLFQNTDSPAVLKTAEAKGKRAFGWDSDMTAYGPKAH
                     LASAVINWGPYYIKATKEALDGSWKGGGASWWGVKEGAIDIVSIAEDVPAETKAKVEE
                     IKKGLADGTFVIWKGPITDNTGKVQVAKDSAADDKFLGGLNFYVKGVEGKIPGGK"
BASE COUNT         1175 a         1670 c         1604 g         1134 t
ORIGIN      
        1 catcccaact ccttggtgca aaacggggag ttattcccga ccacatccta aagaagcaag
       61 ttcgcgcggg gcaagttctg caaaaagggc aagtaacccg ccgagttgca ttcgatggag
      121 accgttttgg agtggatttt ttcgccctga cactggattg agcggcgatt tttgcaaaga
      181 ccgctgcaat aaagccatta aactcgcgcc atgcagaaaa ctatgagaac ttccggcctg
      241 atctgctgtt tattgcttgc ctttgttgcg cacgccgatc ctgccgagtc gccagacgaa
      301 cttggcagct ttctggtcaa taacagcgtt ttgttgcaaa ttgaccaagt gcgtcagagc
      361 gttgcgacca aggcctccaa tctggtggtt aacgcgcttg ggtttttggg cgtgccctac
      421 cgccgggggg gtagttcagc ggaaggcggc ttcgattgca gcggtttcgt caaatcaact
      481 tacgaacaaa cgctggggtt gattctgccg cgcaaggccg agcaacaagc ggcagcgacg
      541 cagcagattg aacggacaga actgcaaccc ggcgacctgg tttttttcaa caccatgcgt
      601 cgtgccttca gtcatgtcgg catttacatt ggcgaaggca agtttgtgca ctcgcccaaa
      661 cctggttccg aagttcgcat tgacaacatg actcagtcct attggagtcg gcgttttgac
      721 ggtgcccgca gggttcaagc actcgcaacc gacgcaaacc ggtagcatcc attgtcagtg
      781 atgccagtgg cgcaaggttg acgcgaacgc ctggcatcag gcaccccttg cagatgatgc
      841 agacatctgt cggacttgcc gggcatcgcc tgtgaaccga ccgcagcggg ccacttttgc
      901 accggctttt gataggttga tgccgactga tctgcaagtg cgtcggcaag gcgttggccg
      961 cagacccgca gatggggttg caggcgaagg ctattgatcg aatcgcgctg cgcgacggtc
     1021 agcttgatct ggctttgttg gtgcagcgct gcaaaatgaa ccagatgctg ccgagatttc
     1081 agctcaacct cgggttactt ggcagagcga gcgcgaaacc cgataacgct tagccaacgt
     1141 tctgacccct tcccaaagcc agcacttgaa ctcaagtgaa gccaccggct cgccattcga
     1201 ctgacggctc actctgcggc atcggatcgg ctgtactgaa cccaagtgaa gccacaggct
     1261 tgacacccca ctttgtgctg cagtcaaatt cacaagatga actggcatct gatctgcaac
     1321 cgtcatgttt tttccttcgc cgccctggct ttttcagcgc cgatgctggc agcagccact
     1381 gggccagcgc tgagcattga tgccgctgcc ggtcagcgcg ccatcaaccc cgacatttat
     1441 ggtgtcaacg agttgtctga cgaggccttg gccaccgaac tgcgcctgcc ggtgcggcgc
     1501 tggggcggca acaccaccag ccgctacaac tggcaaattg acacctacaa caccggcctg
     1561 gactggtatt ttgagaacat accgctagcc aatgccaatg tggcggcctt gcccagcggc
     1621 tcaagcaccg accgctttgt cgagcaaaac cagcgcactg gcagccgcac actgatgacc
     1681 gtgcccaccc tgggctgggt ggccaagcgc cgcaccgacg ggcatccgtt tgactgcggc
     1741 ttcaaggtgt cgcgctacgg agcccagcag tccaccgact actgggaccc cgactgcggc
     1801 aatggcctga ccatgggcgg cagcaacatc accgccaaca acgcgaccga cactagtgtg
     1861 gtcagcaacc cggcctttat cgccgcctgg gtgcaacacc tagtggacaa atatggcacg
     1921 gcggccaatg gcggcgtcag ctactacgcc tttgacaacg agccgatgct gtggcacagc
     1981 acccaccgcg acgtgcaccc gcaacccggc acctacgatg aaatgcgcga ccgcgccctg
     2041 gcctacggtg cagccattaa ggcggtggac ccgagcgcca aaaccctggg gccggtggaa
     2101 gacggctggt gtcgctacct gtattccgcc gccgacgact gcggccctgg cgcagattac
     2161 gcggcgcatg gcagcgttca gtatgtgccc tggtacttgc agcagatgaa gagctacgag
     2221 caacagcacg gcttgcgggt gcttgactat ctggacctgc acaactaccc ggctgccagc
     2281 ggcgtgacac tgtcaccggc cggcaacgcg gccacccagg cgctgcgctt gcgctcgacc
     2341 cgatctttat gggatgccag ctacattgat gagagctgga tttcagacac agcgcccggc
     2401 ggtgtggcgg tgcgcctgat tccgcgcatg aagcaatggg tggccgacca ctaccccggc
     2461 acacgattgg cgatttcgga atacaactgg ggtgcgcccg aaagcatcaa cggtgccctg
     2521 gcgcaggccg acgtgctggg catctttggc cgcgaagggc tggacctggc caccttgtgg
     2581 ggcgaggtgt tcagcgcgca gccactggcc tttgcttttc gcatgtaccg caactacgat
     2641 ggcgccgggc acggttttgg cgacacatcg gtgcaggcca tcagtgccga ccagagcgcg
     2701 ctggccgtct atgccgcccg gcgcagtgcc gacggggcgc tgaccatcat gatcttgaac
     2761 aagtctgcca ctgacttgac gacggctgtc agtctggccg ggttcaaccc aaaagccagc
     2821 gcgcgtgttt ttcgctacag cccggccaac ctgggcgcga ttgagtctcg ggttgctcag
     2881 accgtcagcg ccagcggctt tagcgccagc tttgccgcca actcggtcac cttgctagag
     2941 ctgccacccc aagaccccgc cgtttcactg agcaccacct cgctggcctt tggcaaccag
     3001 accgcaggca ccggcagcac agcccggacg atcaccttga ccaacactgg tggcgcggct
     3061 ttgagcctgg ccatcagcgc acccgccacg tcggcctttt cagtcaccca cgactgcccc
     3121 gccagcctgg cagcaggtgc cgggtgtagc ctgaccgcgc gctttacacc gacaacggca
     3181 ggttctgtca cagatgccat tgtgctgacc accaatgccg caggcagccc acacctcctg
     3241 accttgaccg gccaagggct gccctcggcg gtcaagcggg ttgacctgac accgatcttg
     3301 atgctgctgc tggagtagtg ccgctggcaa gtcacctgtt accgaatgcc gcctgaatcg
     3361 gttggtctgg gcggtgacgg tgctggcttg gggtaagtgc ctaattgcct tgcagcgctt
     3421 attcagcaag cgctgccagc tatcattttt actattctcg acttgtcgca agcggcccgc
     3481 caaaccagaa tggcatgctg ctgctgaccg agtcaaagca ttgcgtcaga ctgccatgct
     3541 gccgagagca ccagaaattt tggaacttgc gcagtgttgg ctgcaaggcg aaggactgcc
     3601 cgccaaagct ggtttgcacg cgctgctcat tgcctgcgct gtctgcggct gcaaactgcc
     3661 tttactggtg acatcctttg aattggtcga gcccattcag tcacatcatt cgccctttgg
     3721 cacaacacga aatttgctga aatccttgcc acgcgcgaac gcctggcgac ccaatcacac
     3781 cgtcctggtg gctaattcct tggatgccca ggcaggttgc agcgccttgg gtctgatgac
     3841 agataccctg tagcacgcac acaagcgcca cctgatccct caattcaagg ctctgccaag
     3901 cctgcgccat tggtttaacg ccgttttgct tgatctggtt gccaagcgtg cagcccggct
     3961 gcgatggctg aaaaagcatc ggtttggcgg cgcgcgggtc aagcccgaca gactcccatg
     4021 cagaacaaat ttaccctgga ctgccaggtt aaacgcagaa cacatttgcc ctgatgggct
     4081 gccggactga gcacggcgac ggttcctcag ttgcattgtg gccttgatct tgcttgatca
     4141 cccgtgattt cgcagcgccg ggtcgctggt cctggctggt cgtgatgtct ggcggtgcat
     4201 tttggccaaa tgtctgatgc tgcgttgata atcgcctgtg tttcttaaac ccgtccccac
     4261 cctttacctt gaaggatttc atatgacaga catgaaaaaa cgttcactta tcagagttgg
     4321 tgccctgagc gctgtggccg ctgctgctgc cttggtgggc tgcggcaaaa aggaagagcc
     4381 ggcacctgcc ccggtagccg ctgcaccggc ttcggcacca gcatcggcac ctgccaaacc
     4441 ggcacccttg aaaatcggct ttgcctatgt tggcccggtt ggcgatggtg gctggacctt
     4501 tgcccatgac aatggccgca agatgctcga aaaagaattt ggcgacaaga tcgtcacctc
     4561 gtttgttgaa aatgtgcccg aatctgccga cgccgagcgg gtgattcgtg acatggcagg
     4621 ccaaggcaac aagctgatct ttggcaccac gtttggttac atggagccga tgctcaaggt
     4681 cgctggcgac ctgaaagatg tcaagtttga gcatgccacc ggctacaagc aggctgacaa
     4741 catgcgcacc tatgacagcc gcacctacga aggtgcctat atggctggtg tgattgccgg
     4801 caagatgacc aagagcaaca cgctcggcgt ggtcgcctcg atcccgattc ctgaagtcat
     4861 tcgcaacatc aacagcttca cactcggtgc gcaaagcagc aaccccaagg tcaagaccaa
     4921 ggtggtctgg gtcaacggct ggtttgaccc gccgaaagaa accgaagccg ccaccagcct
     4981 gatcaatggc ggtgcagacg tgttgttcca gaacaccgat tcgccggctg tgctcaagac
     5041 agcagaagcc aagggcaagc gtgctttcgg ctgggattcc gacatgacag cctatggccc
     5101 caaggcccat ctggcttcag ccgtgattaa ctggggcccg tactacatca aggccaccaa
     5161 ggaagcactt gacgggagct ggaagggcgg cggcgcatcc tggtggggcg tgaaggaagg
     5221 ggccatcgac attgtgtcga ttgctgaaga cgtaccggca gagaccaaag ccaaagttga
     5281 ggaaatcaag aagggcttgg ccgatggcac ttttgtgatc tggaaaggcc cgattaccga
     5341 caacactggc aaggtgcagg tggccaagga ttcggctgct gacgacaaat tcttgggtgg
     5401 tttgaacttc tacgtcaagg gtgtggaagg caagattccg ggcggcaaat gaagtttcct
     5461 tgccagccgg catccgccag acgctgaacg aaaaaccgcc gcaaggcggt ttttttatgg
     5521 caacccagca gagacttgat ctggctcaaa ctgaaaattc acatcgacag ctattttcga
     5581 aga
//
LOCUS       DAOSLL010000285         5581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13058_length_5581_cov_3.894861, whole genome shotgun sequence.
ACCESSION   DAOSLL010000285 DAOSLL010000000
VERSION     DAOSLL010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13058_length_5581_cov_3.894861"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1097)
                     /locus_tag="PLB25_12695"
     CDS             complement(<1..1097)
                     /locus_tag="PLB25_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HOE42471.1"
                     /translation="MEALKQGADALFILLGGIMVLAMHAGFAFLELGTVRRKSQVNAL
                     VKILVDFSVSTVAYFLVGYGVAYGTHFFVGAEQLAAKNGYELVKFFFLLTFAAAIPAI
                     VSGGIAERARFWPQLIATAVVVGVIYPFFEGIAWNQHFGVQAWLKNLTGAEFHDFAGS
                     VVVHAVGGWIALPAVLLLGARSNRYRKDGGISAHPPSSIPFLALGAWILTVGWFGFNV
                     MSAQTLDKISGLVAVNSLMAMVGGTLAALVLGKNDPGFVHNGPLAGLVAVCAGSDLMH
                     PIGALLVGAVAGALFVTMFTLTQNRWKIDDVLGVWPLHGLCGTWGGLAAGIFGSPALG
                     GLGGVSLGAQLIGTAIGVVCAVLGSLLVYGA"
     gene            complement(1299..2144)
                     /locus_tag="PLB25_12700"
     CDS             complement(1299..2144)
                     /locus_tag="PLB25_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2145 domain-containing protein"
                     /protein_id="HOE42472.1"
                     /translation="MGKSQYQAKLPGQFAGALLVVLSLLSGPELAWAGRPCDTPQPPK
                     TQTVERALNLAERTLKSLDASGAQVVLLARAGQDLSKYGLRYSHLGFAYQQTDGAGHR
                     VWRVLHKLNQCGTSVSQIYRQGLGDFFMDDLWRFEAAWVVPTPEVQVKLMALLRDEPR
                     SITLHRKPYSMVSYVWGQKYQQSNQWAIETLALALAPELASAQPTRGQAQAWLQAHNY
                     QPSVLKIGALTRLGGRVTAANVAFDDHPPDKRFSDRIETVTVDSVFAWLPRAGLTSQP
                     VVTQR"
     gene            complement(2219..2677)
                     /locus_tag="PLB25_12705"
     CDS             complement(2219..2677)
                     /locus_tag="PLB25_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42473.1"
                     /translation="MKNLVASYLIAACALLTGATAHFDAKAQSEASALSVISAMPVAS
                     VVAGASVAAGAVLTIPVILATAGTVLVVKTVAATASGTLYLLERASDGAQVSVEVLAR
                     GSAAASVGVGTLATVSVISAGVVLSAAGEVIAFIPNELGRALLYSEQVTR"
     gene            complement(2681..3160)
                     /locus_tag="PLB25_12710"
     CDS             complement(2681..3160)
                     /locus_tag="PLB25_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaA/YiaB family inner membrane protein"
                     /protein_id="HOE42474.1"
                     /translation="MQAGPIVLIQRDSKAWSLQVWLSFGIAIFLCAVGLAYLPGTDLD
                     RVFMVMGYFFCLSAAFVLAKYVRDQEKARQEDRLVDTPMFGFVVWGGFVIAMALTGWG
                     LIRMEVNQTYKAFLGVSWLYLITCTFTLAKTLRDRHEADLLEAKSLSRSYSSNTAKE"
     gene            complement(3286..5190)
                     /locus_tag="PLB25_12715"
     CDS             complement(3286..5190)
                     /locus_tag="PLB25_12715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE42475.1"
                     /translation="MSGHDHPNQFALLKQRRFAPFFWTQFSGAANDNLFKFAFTVMVT
                     YQLSVEWLPPAMAGLVIGALFILPFLLFSATSGQLTDKYDKTVMIRFVKNLEIAIMAL
                     AAYGFITGSVTVLLICTFLMGLHSTLFGPVKFAYLPQVLSEREITGGNGMVEMGTFVA
                     ILLGQVAGGLLVAMPEVGHRDVAISCVVLALLGRLVAQAIPASPATDPGLKINWNPVS
                     ETWRNLKLAHEQLAVFRSLLGISWMWFFGAVFLSQFPSLAKEVLHGNEQVASFLLVIF
                     SVGIGTGSLLCEVLSKRHVEIGLVPLGAIGMSVFSIDLYFALRGLPQAASMGVGTFIG
                     QAQHWRVMLDLLLLSLFAGLYSVPMYALIQMRSQPTHRARIIAANNILNALFMIGSSV
                     IAGALLNSGFSIPQIFLITGIANAVVAFYIFMLVPEYLLRFVAWVMSRLIYRFKVRGD
                     ESIPAQGAAVLVCNHVSFVDAVLLMAASPRPICFLMDHKIFQVPVLGWLFRLAKAIPI
                     APRTEDPRTYEVAFDAAAAVLKEGDLLCIFPEGGLTRDGTLQEFKGGIMKILQRTNAM
                     GLSVPVVPMALTNLWGSYFSRVEQGGAMVRPFRRGMFSRVGLNVGAPMAGSVVQPTLL
                     RERVAQLLAV"
     gene            complement(5177..>5581)
                     /locus_tag="PLB25_12720"
     CDS             complement(5177..>5581)
                     /locus_tag="PLB25_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42476.1"
                     /translation="MAVADRISLRAGLAMLLATLVAGLLSVLVWYQTGNLLAWVVLQG
                     GGMLLALALALRPQVPGGWGLPLVAVLALYTLAKLLELGDHAVFALTQGWVSGHSLKH
                     VVAALAAWPVLAVMHNVAQPGLQLQGESDVRP"
BASE COUNT         1304 a         1804 c         1567 g          906 t
ORIGIN      
        1 attgcgccat acaccagcag gctgcccagg acggcacaga caaccccaat ggcggtgccg
       61 atcagttgcg cgcctaaact gaccccacca agcccaccga gtgccgggct gccaaagatg
      121 cccgccgcca gcccacccca agtgccgcac aggccatgca gtggccagac acccagcacg
      181 tcgtcaatct tccagcggtt ttgcgtcagg gtaaacatgg tgacaaacag cgcaccggcc
      241 accgccccca ccagcaatgc cccgattggg tgcatcaggt ccgaaccggc acacaccgcc
      301 accagcccag ccaatgggcc gttgtgcaca aagccggggt cgtttttgcc cagcaccagc
      361 gcggccagcg tgccacccac catggccatc agtgaattga ccgccaccag gcccgaaatt
      421 ttgtccagcg tttgcgcgct catcacatta aagccaaacc agccgacggt caaaatccag
      481 gcccccagcg ccagaaacgg aatgctgctg ggcgggtgcg ccgaaatgcc gccgtctttg
      541 cggtaacggt tggagcgcgc acccagcaac agcacggctg gcaaggcaat ccagccaccg
      601 acggcgtgca ccaccacgct gccagcaaag tcgtgaaact cggcaccggt caggtttttc
      661 aaccaggctt gcacgccaaa gtgctggttc caggcgatgc cttcgaaaaa tgggtagatc
      721 acacccacaa cgacggccgt ggcaatgagc tgcggccaga aacgggcccg ctcggcaatg
      781 ccgcctgaaa caatcgccgg gattgcggca gcaaaagtca gcaaaaagaa aaacttgacc
      841 aattcgtagc cgtttttggc ggccagttgt tcggctccga caaaaaaatg ggtcccataa
      901 gccacgccgt aaccgaccag aaaatacgcc acggtagaga ctgaaaagtc aaccagaatt
      961 ttgaccagcg cattgacctg gctcttgcga cgcacggtgc cgagctccaa aaatgcaaaa
     1021 ccggcgtgca tggccagcac catgatgccg ccgagaagga tgaacaaggc atctgcgccc
     1081 tgtttgagtg cttccatgag tacctcttgg gagttaattg attgaaattg gtgcgaatgc
     1141 tggtgtcatc cagaaaacgc accaattgaa agcaaaaagc gcgccaggac tgccaagatg
     1201 gtgcgctggt gtgcccagac aggccaattt gcagatcaac ccatggttct tttggagccc
     1261 gtttggcagg gctgcacacc ggcccagagt ctgacaatct agcgctgggt caccaccggc
     1321 tgactggtca ggccggcgcg cggcagccag gcaaacaccg aatcgaccgt gacagtctcg
     1381 atgcggtcgc taaagcgctt gtccggcgga tggtcgtcaa acgccacatt ggccgcagtc
     1441 acgcgtccac cgagtcgggt caaagcacca attttcagca cgctgggctg gtagttgtgc
     1501 gcctgcagcc aggcctgagc ctgaccgcgc gtgggttgag cactggccag ttccggggcc
     1561 aaggctagcg ccagcgtctc aatcgcccat tggttggact gctggtattt ttgcccccag
     1621 acataactga ccatgctgta aggcttgcgg tgcagtgtga tcgaacgtgg ctcatcgcgt
     1681 agcagtgcca tcagcttgac ctgtacctcc ggcgttggca ccacccaggc ggcctcgaaa
     1741 cgccacagat catccatgaa aaaatcgccc agcccctggc gataaatctg cgacaccgaa
     1801 gtgccacact ggttgagttt gtgcaacacc cgccacaccc ggtggccggc accgtcggtt
     1861 tgctggtagg caaagcccag atgcgaatag cgcaagccgt acttcgacag gtcttgcccg
     1921 gcgcgtgcca gcagcaccac ctgcgcgccg ctggcatcga gcgacttgag tgtgcgctcg
     1981 gcgagattca aagcccgctc caccgtctgc gttttcggtg gctgcggggt gtcgcagggt
     2041 cgcccggccc aggcaagctc tggcccggac agcaggctca ggaccaccag caaggcacca
     2101 gcgaactggc ccggcagctt ggcctgatac tgactcttgc ccataagcct gggcgctgcc
     2161 tgcaactcgc cgcttgcgcc tttgcgccag ggggcaagcc acccgtgacg cggcactttc
     2221 atcgcgtcac ctgctcgctg tacagcagcg cccggcccag ttcattgggg atgaaggcta
     2281 tgacctcacc tgccgccgac aagaccaccc cggcgctgat cacgctgacc gtggccaatg
     2341 tgccaacgcc gaccgaggcg gcggcactgc cacgggccag cacctcgaca ctgacctgtg
     2401 caccgtccga ggcacgctcg agcagataca gcgtgccgct ggcggtcgcg gcaaccgtct
     2461 tgaccaccag caccgtgccg gcagtggcca atatcaccgg aatcgtcagc accgcgcctg
     2521 ctgccacgct ggcacccgcc accaccgagg ccaccggcat cgccgagatc acgctcaagg
     2581 ccgatgcctc gctttgcgcc tttgcatcaa aatgggctgt agcccccgtc agcaatgcgc
     2641 aagcagctat caaatatgaa gcaactaggt ttttcatggc ttactccttg gcagtgttgc
     2701 tgctgtatga gcggctcagg cttttggctt caagcaggtc tgcctcatgc cggtcacgca
     2761 gggtcttggc caacgtgaag gtgcaggtaa tcagatacag ccagctcacc cccagaaacg
     2821 ccttgtaggt ctgattgacc tccatgcgga tcaggcccca gccggtcagg gccatggcga
     2881 tgacaaagcc gccccaaacc acaaagccaa acatcggggt atcgaccagg cggtcttcct
     2941 ggcgggcctt ttcctgatca cgcacatact tggccagcac aaacgccgcc gacaggcaaa
     3001 aaaagtaacc catgaccatg aagacccggt ccagatcggt gccaggcaga taggccagcc
     3061 cgactgcgca caagaagatg gcgatgccaa acgacagcca gacctgcagg gaccaggctt
     3121 tggaatcacg ctgaatcaga acaatggggc cagcttgcat ttgatttctc cgggtgttgt
     3181 tgaaagggaa gcacagttta gaaaaagcgc agacaaagga gggcgaaaaa ctaaaccagt
     3241 cttataaaat aattgccttg gtatcaaaaa acgatacttg gcaagtcaaa ccgcgagcag
     3301 ttgggccacc cgctcgcgca acagggtcgg ctgcaccacg ctgccagcca ttggcgcacc
     3361 gacattcaag ccgacccggc tgaacatgcc acgccggaac ggtcgcacca tcgcaccgcc
     3421 ctgctcgacc cggctgaagt atgagcccca caggttggtc aaggccatcg gcaccaccgg
     3481 cacgctcaag cccatcgcat tggtacgctg cagaattttc atgatgccgc ccttgaactc
     3541 ctgcagcgtc ccgtcgcgcg tcaaaccccc ttcgggaaag atgcacagca aatcgccttc
     3601 cttgagcacc gccgcagcgg catcaaacgc cacctcatag gtgcgcgggt cttcggtccg
     3661 cggcgcgatc ggaatcgcct tggccagccg aaacagccag cccagcaccg gtacctgaaa
     3721 aatcttgtgg tccatcagaa aacaaatcgg ccgcgggctg gctgccatca gcagcaccgc
     3781 atcgacaaaa ctcacatggt tgcagaccag taccgcagca ccctgggcgg gaatggactc
     3841 gtcgccacgc accttgaagc ggtaaatcag gcgcgacatg acccaggcaa caaagcgcag
     3901 caggtactcg ggcaccagca tgaagatgta aaacgccacc accgcattgg caatgccagt
     3961 gatcaaaaag atctgcggga tggaaaagcc gctgttgagc aaggctccgg caatcaccga
     4021 gctgccaatc atgaacaagg cgttaaggat gttgttggcg gcaatgatgc gggcgcggtg
     4081 cgttggctgg cttcgcatct ggatcagcgc gtacatcggc acactgtaga gaccggcaaa
     4141 caggctgagc aacagcaggt caagcatcac ccgccagtgc tgcgcctggc caataaaagt
     4201 gccaaccccc atgctggccg cttgcggcaa accgcgcagg gcaaagtaga ggtcaatcga
     4261 aaacacgctc atgccgatcg caccgagcgg caccaggcca atttcgacat ggcgcttgct
     4321 caggacttcg cacaacagcg agccagtgcc aatgccgacc gaaaaaatca ccagcaggaa
     4381 cgaagccacc tgctcattgc cgtgcaacac ctctttggcc aggctgggga actggcttag
     4441 aaacaccgcg ccaaaaaacc acatccaact gatgcccagc aaagagcgaa acaccgccag
     4501 ttgctcatgt gcgagtttca ggttgcgcca ggtttccgac accgggttcc agttgatctt
     4561 caagccgggg tcggtggctg gcgaggccgg aatcgcctgc gccaccagcc gccccagcag
     4621 cgccagcacg acacaggaaa tcgccacatc gcggtgaccc acctcgggca tggcaaccag
     4681 cagtccaccg gccacctgac cgagcaaaat cgcgacaaaa gtgcccatct cgaccatgcc
     4741 attgccgccg gtgatctcgc gctcgctcaa gacttgcggc agataggcaa acttgaccgg
     4801 gccaaacagt gtggagtgca ggcccatcaa aaaagtgcag atcagcagca ccgtgacgct
     4861 gccggtgatg aatccgtaag ccgccagcgc catgatggcg atttcgaggt ttttgacaaa
     4921 tcggatcatg acggtcttgt catacttgtc ggtcaactgg ccgctggtgg ctgaaaacag
     4981 caggaatggc aggatgaaaa gcgccccaat caccagtccg gccatcgccg gtggcaacca
     5041 ttcgacgctg agctggtagg tcaccatcac ggtaaaggca aacttgaaca ggttgtcgtt
     5101 cgcagcgccc gaaaactggg tccagaagaa cggcgcaaaa cggcgttgct taagcagcgc
     5161 aaactggttg gggtgatcat ggccggacat cggattctcc ttgcagttgc aaacctggct
     5221 gggcgacatt gtgcatgacc gccagcaccg gccaggccgc cagtgccgcc accacatgtt
     5281 tcaggctgtg gcccgacacc cagccctgcg tcagggcaaa taccgcgtgg tcgcccagct
     5341 ccagcagctt ggccaatgtg tacagcgcca gcaccgctac caacggcagg ccccagccgc
     5401 cgggcacctg gggacgcagc gccagggcca gcgccagcag catgccaccg ccctgcagca
     5461 ccacccaggc cagcaaattg ccagtctggt accacaccag caccgacagt agcccggcca
     5521 ccagggttgc cagcagcatg gccaggccgg cacgcaggct gatgcggtct gccacggcca
     5581 t
//
LOCUS       DAOSLL010000286         5547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13144_length_5547_cov_4.752549, whole genome shotgun sequence.
ACCESSION   DAOSLL010000286 DAOSLL010000000
VERSION     DAOSLL010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5547
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13144_length_5547_cov_4.752549"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..4161
                     /gene="hrpA"
                     /locus_tag="PLB25_12725"
     CDS             43..4161
                     /gene="hrpA"
                     /locus_tag="PLB25_12725"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HOE42477.1"
                     /translation="MPAPLNIEFPESLPVSARRAEIMAAMRQHQVIIVCGETGSGKTT
                     QLPKIALALGRGLCNYPKTLADGRPAPRGKLIGHTQPRRIAASSVAKRIAEELKTTPG
                     EVVGFKVRFQDRLGRDASVKLMTDGILLAETQTDPLLQAYDTIIIDEAHERSLNIDFL
                     LGYLKQILPRRPDLKVVVTSATIDAQRFADHFASKTGPAPIIMVSGRMFPVEQRYRPF
                     DEARDYNLNNAIADAVDELWRDVHNSGDILVFLPGEREIREAADHLRGHLSHQPIFRN
                     AEVLPLFARLNQAEQDRIFDSHSGRRIVLATNVAETSLTVPGIRYVIDAGTARMKRYS
                     FRSKVEQLLVEPISQSSANQRAGRCGRVANGICIRLYDEKDFDARARFTEPEILRSSL
                     AGVILRMKALRLGVVEDFPFIESPSGRAIADGYQLLSELGAVDDANELTATGQELAKL
                     PLDPRVGRMILEARSRHALDEVLVIASALSVQDVRDRPMEAQQQADQAHAKFDDEKSE
                     FSGYLKLWQWLESNRGGNWGQSRIKSPESKTENNSTLTPINLAAPKLSNRQYEQLLRQ
                     NFVSMRRVREWRDIHSQLHTVVAEHKWRLNTQPASYEQLHLSMLAGLLGNIGCKLETD
                     AASGDYLGARGIKFYPHPGAHLVKKPGRWIVAAELVETTRLFGRGIAAIEPQWLEQVG
                     AHLLKKQLLDPHWEKKAAEVNALERATLYGIVIYSGRRVNYSRVDLVGAREIFIREAL
                     VGGQWDTRLPFLAANQKLIKQVEDLEHKARRQDVLVDDELIYAFYDQQLPADVCSGFS
                     FEAWYREAARINPRLLLLTREELMRHEAAGITTQSFPKTIRLGGVDCAASYLHEPGDP
                     KDGLTVTVPIFVLNQVNEERCEWLVPGMLKDKIQALLKTLHQRPRSRLVPLPESARKM
                     AEQLNEPALFGHGSLVEAVLKLVRLATAVDVARADIKVDMLQPHFFMNLRVVDEHGRQ
                     LGAGRNLGALRADLGALARGAFQALAGLKLGPGGGASAAAGLYKENGAPAHTQQALTA
                     INDVASRQLLGEPPQRLLKNASKQAVTPVKHVQQAIKSEAYQALPALAGQRHTGWTFG
                     ELPELLEIQKGGQTLIGYPALLDAGDAVTIEVFDEPDVAAARHRAGLRRLFALQLKDA
                     LKYLEKNIPDLQKMAVTFMQVGKAADGTNASASGGTMEELREQIIGLALDRAFLLDPL
                     PTDELAFKRRVDEGRGRLTLIATEVARLAATILNEYAVAARKIKDSKTAADAAADAAG
                     QLARLMPRRFLTLTPWGALQHYARYQKAITARLEKYRSDPARDAARLAELRPLEQRYW
                     RLVAERKGVMDERLQEFRWLLEELRVSFFAQELRTPQPVSVKRLEKAWAQLG"
     gene            complement(4410..>5547)
                     /gene="rpoD"
                     /locus_tag="PLB25_12730"
     CDS             complement(4410..>5547)
                     /gene="rpoD"
                     /locus_tag="PLB25_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOE42478.1"
                     /translation="KTQRALSDELMSIRFTAKTIEKLCDMVRGQVDDVRKKERELRRI
                     IVDKCGYPQVQFITEFSGRDKHHNKVTSHLLDLKWIEKQAAAGKSWSAIMGRNIPPVQ
                     ELQQRLADLQAKVVVPLDQLKDINKRMNEGEYASRAAKKEMIEANLRLVISIAKKYTN
                     RGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPV
                     HMIETINKMNRISRQHLQEFGYEPDASVLAERMEIPEDKIRKIMKIAKEPISMETPIG
                     DDDDSHLGDFIEDGANTAPMEAAMQAGLRDVVKDILDSLTPREAKVLRMRFGIEMTND
                     HTLEEVGKQFDVTRERIRQIEAKALRKLKHPSRSDKLRSFTDNL"
BASE COUNT         1115 a         1652 c         1680 g         1100 t
ORIGIN      
        1 tcgtcaggcg ctccctgaca cacactcgat aattgcggcc ccatgcccgc acctttgaac
       61 attgagtttc ccgagtcgct accggtcagc gctcgccgcg ccgagatcat ggcggcgatg
      121 cgccagcacc aggtcatcat cgtctgcggt gaaaccggct ccggcaaaac cacccaactg
      181 cccaaaattg cactggcgct gggacgcggc ctgtgcaact accccaagac cctggccgat
      241 ggccggccgg caccgcgcgg caagctcatc ggtcacaccc agccgcggcg cattgcggcc
      301 agttcggtcg ccaaacgcat cgccgaagag ctgaaaacaa cgcctggcga ggtcgttggc
      361 ttcaaggtgc gctttcagga cagattgggc cgcgatgcct cggtcaagct gatgaccgac
      421 ggcatcttgc tggcagaaac ccagaccgac ccgctgctgc aagcttacga cacgatcatc
      481 atcgatgagg cccacgagcg cagtctgaac atcgatttcc tgctagggta tttgaagcag
      541 atcctgccac gcaggcctga tttgaaagtg gtggtcacct ccgccacgat agatgcccag
      601 cgttttgccg accactttgc cagcaaaaca ggaccggcac cgatcatcat ggtgtccggg
      661 cgcatgttcc cggtcgagca gcgctaccgc ccgtttgatg aagcacgcga ctacaacctc
      721 aacaacgcga ttgccgatgc ggtggacgag ttgtggcgcg atgtgcacaa cagcggcgac
      781 atcctggtgt ttttgccggg cgagcgcgaa atccgcgagg ccgccgacca tctgcgcggg
      841 cacctgagcc atcagccgat ctttcgcaat gccgaggtgc tgccgctgtt tgcccgcctc
      901 aaccaggccg agcaggaccg gatttttgac agccacagcg gccggcgcat tgtgctggcc
      961 accaatgtgg ccgaaacatc gcttaccgtg ccaggcatcc gctacgtcat tgacgcaggc
     1021 acagcgcgca tgaagcgcta tagcttcaga agcaaggtgg agcaattgct ggtagaaccc
     1081 atcagccaaa gttcggccaa ccagcgcgcc gggcgttgcg gccgggtggc caacggcatc
     1141 tgcattcggc tctacgacga aaaagacttc gatgccagag cgcgcttcac cgaacccgag
     1201 atactgcgtt catcgctggc gggtgtcata ttgcgcatga aggcgctgcg cctgggtgtg
     1261 gtcgaagact ttccgttcat cgagagtcca tccgggcgcg ccattgccga cggttaccag
     1321 ttgctgagcg aactcggcgc ggttgacgat gccaatgaac tcacagcgac gggccaggaa
     1381 ctggccaaac tgccacttga cccgcgggtc ggtcgcatga ttctggaggc acgcagccga
     1441 cacgcgctcg acgaagtgct ggtcattgcc agcgccttga gcgtgcagga tgtgcgcgat
     1501 cggcccatgg aggcgcaaca gcaagccgat caggcacatg ccaagtttga cgatgaaaaa
     1561 agtgagtttt ccggctactt gaagctatgg cagtggctag aaagcaaccg aggaggtaat
     1621 tggggtcaga gtcgaattaa atcgccagaa tcaaaaaccg agaataattc gactctgacc
     1681 ccaattaatc ttgctgcgcc caagctcagc aaccgccagt acgagcaact gctgcggcaa
     1741 aactttgtca gcatgcgccg ggtgcgggag tggcgcgaca tccatagcca gcttcacacg
     1801 gtggtggctg agcacaaatg gcggctcaac acccagcctg ccagttatga gcaactgcac
     1861 ctgtcgatgt tggccgggct gttggggaac atcggctgca agcttgaaac cgacgccgcc
     1921 agcggcgact acctgggcgc gcgcggcatc aagttttacc cgcaccccgg tgcgcacctg
     1981 gtcaaaaaac cgggccgctg gattgtggcc gctgagctgg tggaaaccac ccgtctgttt
     2041 ggccggggca tcgccgccat cgagccgcaa tggctggagc aagtcggcgc acacctgctg
     2101 aaaaaacagt tgctcgaccc gcactgggag aaaaaggcgg cagaagtgaa tgcgctggag
     2161 cgcgccacgc tgtacggcat cgtgatttac agcggccggc gggtcaacta cagccgggtc
     2221 gatctggtcg gggcgcgtga aatcttcatc cgcgaggccc tggtcggcgg ccagtgggac
     2281 accaggttgc cgtttttggc cgccaatcag aagctgatca agcaggtcga agacctggag
     2341 cacaaggcgc gccgacaaga tgtgctggtc gatgacgagt tgatttacgc cttttacgac
     2401 cagcaattgc ccgccgatgt ctgcagcggt ttcagctttg aggcctggta ccgtgaagcc
     2461 gcccggatca accccaggct gctgctgctg acgcgcgaag agttgatgcg ccacgaggcg
     2521 gccggcatca ccacccagtc tttccccaaa accatccggc tcggcggggt cgattgcgcg
     2581 gccagttacc tgcacgaacc gggcgatcca aaagatggcc tgaccgttac cgtgccgatc
     2641 tttgtgctca atcaggtcaa cgaggagcgt tgtgaatggc tggtgcccgg catgctcaag
     2701 gacaaaatcc aggcgctgct gaaaaccctg caccagcgac cccgttcgcg cctggtgccc
     2761 ttgcccgagt cggcccgcaa aatggccgag caactcaatg agcccgcctt gtttggccat
     2821 ggctcgctgg ttgaagcggt gctcaaactg gtgcgcctgg ccacggcggt ggatgtggcg
     2881 cgcgccgaca tcaaggttga tatgctgcag ccgcacttct tcatgaactt gcgggtggtc
     2941 gatgaacatg gccggcaact gggtgccggg cgtaacctcg gtgccctgag ggccgatctg
     3001 ggggcgctgg cgcggggcgc gtttcaggca cttgccgggc tcaagctcgg gccgggcggt
     3061 ggtgccagtg ctgctgccgg tttatacaag gaaaatggtg ctccagccca tacacagcaa
     3121 gcgctgacag ctattaatga tgtagcaagt cggcagcttt tgggtgaacc gccccaacgg
     3181 cttttaaaaa atgcatcaaa acaggctgta acccccgtca aacatgtcca acaagctatt
     3241 aaatctgaag catatcaagc acttccggcg cttgccgggc agcgccacac cggttggaca
     3301 tttggcgagt tgcccgagtt gctggagatt caaaaaggtg gccaaacgct gattggctac
     3361 cctgccctgc tggacgcagg cgatgccgtc accattgagg tgtttgacga gcccgatgtg
     3421 gctgcagccc ggcaccgtgc cggtttgcgt cgtctgtttg cgctgcaact caaggatgcc
     3481 ctcaaatacc tggaaaagaa cattccggac ctgcagaaaa tggcagtcac tttcatgcag
     3541 gttggcaaag cggccgatgg cacgaacgca tcggccagcg gtggaaccat ggaggagttg
     3601 cgcgagcaaa tcatcgggtt ggcgctggac cgggcctttt tgctcgaccc gctgcccacc
     3661 gatgaattgg ccttcaagcg ccgcgtcgat gagggccgtg gccgcctgac cctgattgcg
     3721 accgaggtag cgcggctagc cgccaccatc ttgaacgaat acgcggtggc cgctcgcaag
     3781 atcaaggaca gcaaaaccgc agccgatgca gccgcagatg ccgccgggca actggcacgc
     3841 ctgatgccac ggcggttttt gacgctgacc ccctggggcg cgctgcagca ctatgcccgc
     3901 taccaaaaag ccatcaccgc ccggctggag aaataccgca gcgacccggc ccgcgatgcg
     3961 gcgcgactgg ctgagctgcg cccgctggag cagcgctact ggcgactggt ggccgagcgc
     4021 aagggggtga tggacgagcg gctgcaggaa tttcgctggt tgctggaaga actgcgggtc
     4081 agcttcttcg cgcaagaact gcgcacaccg cagccggtca gcgtcaagcg gctggagaaa
     4141 gcctgggcgc agttgggttg aaggcgtgcc agaggttggc cggttccgct tgatgttgca
     4201 aagcccttga ccagattagc gcaatctgcc tgtccgcatc ctggcaaaaa tggagcctgt
     4261 cataagctat ataaaatgta gctggcagcg caatcaggac gtgccctgca ggctgatttg
     4321 ccatcagtta accaggtgcc ctgtgcgacc tccgccgtgg caaccttgct atttttttgg
     4381 aaagaacgac tggtgccggg ccgggcggct cacaggttat cggtaaagct gcgcagcttg
     4441 tccgagcggc tcgggtgctt gagttttcgc agcgccttgg cttcaatctg gcgaatgcgc
     4501 tcacgcgtca cgtcaaactg cttgcccacc tcttcgagcg tgtggtcatt ggtcatctcg
     4561 atgccaaagc gcatgcgcag caccttggct tcacgcgggg tcaggctgtc gagaatgtcc
     4621 ttgaccacgt cgcgcagacc agcctgcatg gcggcttcca tcggcgcggt gttggccccg
     4681 tcttcgatga aatcgcccag gtggctgtcg tcgtcgtcac caatcggggt ttccatggaa
     4741 atcggctctt tggcgatttt catgatcttg cggatcttgt cctcgggaat ctccatgcgc
     4801 tcagccagca ccgacgcgtc tggctcatag ccgaactctt gcaggtgctg acggctgatg
     4861 cggttcatct tgttgatggt ttcgatcatg tgcaccggaa tgcggatggt gcgcgcctgg
     4921 tcggcaatcg agcgggtgat ggcctggcga atccaccagg tggcataggt tgaaaacttg
     4981 tagccgcgcc ggtattcgaa cttgtcgacc gccttcatca ggccgatgtt gccttcctgg
     5041 atcagatcga gaaactgcaa gccacggttg gtgtattttt tggcaatcga gatcaccagc
     5101 cgcaaattgg cctcgatcat ttcctttttg gcagcgcgcg aggcgtattc gccttcattc
     5161 atgcgcttgt tgatgtcttt caactgatct agcggcacca ccaccttggc ttgcaggtca
     5221 gccaggcgct gctgcaactc ttgtaccggc gggatgttgc ggcccatgat ggcgctccag
     5281 gatttgccag ccgccgcctg cttttcaatc catttcaggt cgagcaggtg gctggtcacc
     5341 ttgttgtggt gcttgtcacg accgctgaac tcggtgatga actgcacttg cgggtagccg
     5401 cacttgtcca cgatgatgcg gcgcagttcg cgctctttct tgcgcacatc atcaacctgg
     5461 ccgcgcacca tgtcgcacag cttttcgatg gtttttgcgg tgaaccggat cgacatcagc
     5521 tcatctgata aagccctttg ggtcttg
//
LOCUS       DAOSLL010000287         5531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13196_length_5531_cov_3.879109, whole genome shotgun sequence.
ACCESSION   DAOSLL010000287 DAOSLL010000000
VERSION     DAOSLL010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13196_length_5531_cov_3.879109"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="PLB25_12735"
     CDS             <1..871
                     /locus_tag="PLB25_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HOE42479.1"
                     /translation="STWFDGGFLDLRRLPWNSPASLIEKLIKYESVHDIKSWADMKNR
                     LDSDRRCYGFFHPSLPNEPLIFVEVALVDAIANSITPLLDESADAVNLNKATTAIFYS
                     ISNTQVGLRGVSFGDSLIKHVVDVLKAEFPHLKTFATLSPIPGFRAWLGKNVASMMTR
                     LDERTRTELKQALQSNTELTAQLLTDAQKSKTLDAKSPVGRFLLQCAAHYLSQELVNG
                     KAIDPVARFHLGNGARIERLNWGADPSIKGVKQSCGMMVNYLYDLKRLDKHRAQLAQG
                     KIPVSREFESLKF"
     gene            1085..1423
                     /locus_tag="PLB25_12740"
     CDS             1085..1423
                     /locus_tag="PLB25_12740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42480.1"
                     /translation="MIRIDGVWLATQPPDMRAGINAVLARVANVFGAAHTASLFVNQP
                     ANRLEVLGQALHAPLMSAAFEIGIGPSTARHPWRKWIASAAPTNQEGVGDFGLNLRSR
                     VGIAFLGAYF"
     gene            complement(1373..1684)
                     /gene="fdxB"
                     /locus_tag="PLB25_12745"
     CDS             complement(1373..1684)
                     /gene="fdxB"
                     /locus_tag="PLB25_12745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007177220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin III, nif-specific"
                     /protein_id="HOE42481.1"
                     /translation="MSNTFSVTLPSGESWTPTFVTALDDQKCIGCGRCFKVCPRGVLE
                     LVGLTEDGERIAVAVDDDDDDEEYEKKVMTIAHQELCIGCTACSKICPKKCYSHAAAE
                     V"
     gene            complement(1711..1911)
                     /locus_tag="PLB25_12750"
     CDS             complement(1711..1911)
                     /locus_tag="PLB25_12750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCE_0567 family metalloprotein"
                     /protein_id="HOE42482.1"
                     /translation="MSTDAEELKLRLRKLNARATQAKMDLHDLSEELPTNWEQILPVA
                     QRCHEAHALLMEARQMAAAAGI"
     gene            complement(1914..2408)
                     /locus_tag="PLB25_12755"
     CDS             complement(1914..2408)
                     /locus_tag="PLB25_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifX-associated nitrogen fixation protein"
                     /protein_id="HOE42483.1"
                     /translation="MTAIDTPAAVTVATADEACLATPFVQQLIKQLRAQDINGTWDSK
                     SDLQLLKPYIHTAEERRALPILGDPDPETLWHLEIFYNAVAVAIERETGQMVSPMMKM
                     SHEGFGRMVLLAGRLVVVNKQLRDVHRFGFTSLAKLAEAGTRFFDEAVTMIRTYPAVA
                     QYGA"
     gene            complement(2405..2815)
                     /gene="nifX"
                     /locus_tag="PLB25_12760"
     CDS             complement(2405..2815)
                     /gene="nifX"
                     /locus_tag="PLB25_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifX"
                     /protein_id="HOE42484.1"
                     /translation="MKVAFTTQDKEHVDAHFGWAKSIVVYDVSPAGHHLVETFEFGDL
                     LEEDGDEDKLSPKLEAIKDCAIVYVAAIGGSAAARVVALKIHPVKVPVPEPIAGILDK
                     LQAVLGGTPPPWLRKAMAKDGVRTFNFEEDEVTT"
     gene            complement(2885..4258)
                     /gene="nifN"
                     /locus_tag="PLB25_12765"
     CDS             complement(2885..4258)
                     /gene="nifN"
                     /locus_tag="PLB25_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifN"
                     /protein_id="HOE42485.1"
                     /translation="MASVVESKKACTVNPLKMSQPLGASLAFLGLDACMPVMHGSQGC
                     TSFGLVLLVRHFKEAIPLQTTAMNEVSSILGGYDNIEAALLNIRKRAAPKIIAICSTG
                     LTETKGDDVDGYIVTVRKRKPELADTEIIYASTPDYVGGFEDGYKHAVTAIARALVKP
                     LPVNPKQVTLLPGSHLSPGDIDELREIIEAFGLSCIVLPDISGSLDGHIAPDWRGTTL
                     GGTTLEQIRAAGASALTLGVGEQTREAAEALNGIAGTALEIFDRLTGLEVNDRFLQRL
                     AQVSGRPVPAKYRRQRSQLLDAMLDGHFYTGGMKVAIAAEPDLLLAVGCLLHEMGAEL
                     RCCVSTTKSASHALLPAAQVILGDLEDLERGAVDCDLLVTHSHGRQAAERLHKPLLRI
                     GFPVFDRVGNAHRCQVGYRGSMNLIFDIANLMIDQIEHHHASDWPLTPEALRAATTVP
                     AAQPCAC"
     gene            complement(4285..>5531)
                     /gene="nifE"
                     /locus_tag="PLB25_12770"
     CDS             complement(4285..>5531)
                     /gene="nifE"
                     /locus_tag="PLB25_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrogenase iron-molybdenum cofactor
                     biosynthesis protein NifE"
                     /protein_id="HOE42486.1"
                     /translation="SSGPQIYRSGFTTDTNELDVIYGGEKRLFKSIREILEKYNPPAV
                     FVYQTCVTALVGDDIEAVCQRATEKFGKPIIAVNAPGFAGIKNLGNKLGAEALLDYVI
                     GTVEPEFTTPYDINIIGEYNLVGELWQVKPLLDGLGIRILSCIAGDGRYNDIASAHRA
                     RVNMMVCSKSMINIATKMEQRWGIPYFEGSFYGIGDMSDTLRQIAKVLVQQGAPDDLL
                     GRTERLIEVEEARAWQRIEHFKQRLAGKRVLLITGGVKSWSVVAALQEAGMEIVGTSV
                     KKSTRQDKEKIKEIMGDDAHMIDNMTPREMYDMLRDARADIMLSGGRSQFVALKARMP
                     WLDINQERHHPYGGYEGMVELVREIDRAIYNPVWQQVRIPAPWGDDGETLGAVLPSAR
                     PMDLLTGVAARSMDHSAEELPV"
BASE COUNT         1101 a         1663 c         1590 g         1177 t
ORIGIN      
        1 ttccacctgg tttgacgggg gttttttgga cctgcgccgc ctcccatgga actcgccggc
       61 ttcgttgatc gaaaaattga tcaagtacga gtcggtgcac gatatcaaaa gctgggccga
      121 catgaagaat cggctcgact cagatcgccg ctgctacggt ttttttcatc ccagcctacc
      181 caatgaacct ttgatctttg tcgaagtggc cctcgttgat gccattgcaa atagcatcac
      241 acccttgctc gatgagtcgg ctgacgcagt caacctcaac aaggccacaa cggcgatttt
      301 ttactccatt agcaacacgc aggtcggact gcgcggggtg agttttggcg attcgttgat
      361 caagcatgtg gtcgatgtgc tgaaggcaga gtttccccac ctcaagacct ttgccacctt
      421 gtcgccgatt cccggctttc gcgcctggct tggcaaaaat gtcgccagca tgatgacccg
      481 acttgatgaa aggacgcgca cagaacttaa gcaggcgttg caatcgaata ccgaattgac
      541 cgcgcagttg ctcactgatg ctcaaaaatc gaaaaccctt gatgccaagt cgccggtggg
      601 ccggttcttg ctgcaatgcg ccgcccacta cctgagccag gaactcgtga atggcaaggc
      661 aattgacccg gtggcgcgtt ttcacttggg caacggtgct cgtattgagc gcctgaactg
      721 gggtgctgac ccgtcgatca agggcgtgaa acagtcatgc ggcatgatgg tcaattacct
      781 ctacgacctt aaacgtctcg acaaacaccg cgcccagttg gcgcaaggca agattccggt
      841 ctcacgcgag tttgaaagcc tgaaattctg aattcggtgg tgcattggcc acaattcaat
      901 agcgcccaat aaggcgttgc tcaaaacagg ggcacaagcg tagcgcagaa caagtaccac
      961 gcctgttaca tcgggtcatg catcacgtgc aaactatgct gatcgacggc aacacgggcc
     1021 tgcacctcca ccgggcagaa agcgcctcaa gccaatctgc cgggcggctg cacgaggtgc
     1081 tggcatgatc cgcattgatg gcgtatggct ggcgacccag cccccggaca tgcgcgctgg
     1141 catcaacgcc gtcttggccc gcgttgccaa tgtcttcggt gccgcccaca ccgcctccct
     1201 gtttgtcaac caacccgcca atcgcctgga agtgctgggg caggccctcc acgcgccgct
     1261 gatgtcggcc gcatttgaaa ttggtatcgg cccaagtacc gcaaggcacc cgtggcgcaa
     1321 atggattgct tctgccgcgc cgactaacca agaaggtgtt ggagatttcg gcttaaacct
     1381 ccgcagccgc gtgggaatag catttcttgg ggcatatttt tgaacacgca gtgcagccta
     1441 tgcacaattc ctgatgcgcg atggtcatca ccttcttttc gtattcctca tcatcatcgt
     1501 catcgtcaac agcgactgcg atgcgctcgc cgtcttcagt tagcccgaca agttcaagca
     1561 cgccgcgcgg gcagaccttg aaacagcggc cacagccgat gcatttctga tcgtcgagcg
     1621 cggtgacaaa ggtgggggtc cagctttcgc cgctgggcaa ggtgacgcta aaggtgttgc
     1681 tcatggcagg atatccttgt tgatctggtg tcaaatgcct gctgctgcgg ccatttgccg
     1741 ggcttccatc agcagcgcat gggcttcgtg gcaccgctgg gccactggca ggatctgttc
     1801 ccaattggtc ggcaattctt cggataaatc atgcaggtcc atcttggcct gggtggcgcg
     1861 ggcgttgagt tttctcagac gcagcttgag ttcttcggca tccgtgctca tgttcaggct
     1921 ccgtactgcg caacagcagg gtaagtgcgg atcatcgtca ccgcctcgtc gaaaaaccgc
     1981 gtgccagcct cggccagttt ggccagcgac gtaaagccga agcggtgcac gtcacgcaac
     2041 tgtttgttta ccaccaccag gcgcccggcc agcagcacca tacggccaaa accctcgtgg
     2101 ctcatcttca tcatgggcga caccatctgg ccggtttcgc gctcgatggc gacggccacc
     2161 gcgttgtaga agatctccag atgccacagg gtctcggggt ccgggtcgcc caggatcggc
     2221 aaggcgcgcc gctcttcggc agtgtgaata tagggcttga gcaattgcag gtcgctcttg
     2281 ctgtcccagg tgccgttgat gtcctgtgcc ctcagttgct tgatgagctg ctgcacaaag
     2341 ggtgtggcga gacaggcctc atcagccgtg gcaactgtca ccgcagccgg ggtgtcgatt
     2401 gcagtcatgt ggtgacctcg tcttcttcaa aattgaaggt gcgcacgcca tccttggcca
     2461 tggccttgcg cagccagggc ggcggcgtgc cccccagaac cgcttgcagc ttgtccagaa
     2521 tgccagcaat gggctcgggc accggcacct ttacaggatg gattttcaaa gccaccacgc
     2581 gtgccgccgc cgagcccccg atggctgcca catacacaat tgcgcagtcc ttgatggctt
     2641 cgagctttgg gctgagcttg tcttcgtcgc cgtcttcttc gagcaggtca ccaaactcga
     2701 aggtctcaac caaatgatgc cctgctggcg acacgtcata cacaacgatg cttttggccc
     2761 agccaaagtg ggcatcgacg tgctctttgt cctgggtggt aaaggcgact ttcataaaac
     2821 tctcctggtg tggggatggg ttgaaccaac aaatgagtga cggtcagcgg atacctgccg
     2881 ccgatcagca agcacaaggc tgcgccgccg ggacggtggt ggcggcgcgc agggcttcgg
     2941 gtgtcagcgg ccagtcgctg gcatggtgat gttcgatctg gtcaatcatc aggttggcga
     3001 tgtcaaagat caggttcatg ctgccccgat agccaacctg gcaacggtgc gcgttgccaa
     3061 cgcggtcaaa caccggaaaa ccaatgcgca gcagcggttt atgcaaacgc tctgcagcct
     3121 ggcgaccgtg agaatgggta accagcaagt cgcaatctac cgcgccgcgc tccaggtctt
     3181 ccaggtcacc caaaattacc tgcgcggcgg gcagcagtgc gtgtgatgcc gacttggtgg
     3241 tgctgacgca gcagcgcaac tcagcaccca tctcgtgcaa aaggcagcca acggccagca
     3301 gcaggtcggg ctcggcagca atcgccacct tcatgccacc ggtgtaaaag tggccatcca
     3361 gcatggcatc gagcaactgg ctgcgctggc ggcggtactt ggctggcacc ggtcgacctg
     3421 agacctgcgc cagccgttgc aaaaagcggt cattgacttc cagtccggtc aaacggtcaa
     3481 aaatctccag tgccgtaccg gcaatgccat tgagcgcctc ggcggcttcg cgggtctgtt
     3541 cgcccacgcc caatgtcagg gccgaggccc cggcggcacg aatctgttcc agcgttgtgc
     3601 cgcccagcgt ggtgccgcgc cagtccggcg caatgtggcc gtccagcgag ccggaaatat
     3661 caggcaacac aatgcacgat aggccaaagg cttcaatgat ctcgcgcagt tcgtcaatgt
     3721 cacccggtga caggtggctg ccgggcagca gcgtgacttg cttcgggttg accggtaacg
     3781 gcttgaccag cgcccgggcg atggcggtca ccgcatgttt gtagccgtct tcaaagccgc
     3841 ccacatagtc gggagtcgag gcgtagatga tttcggtgtc ggccagttcc ggcttgcgct
     3901 tgcgcaccgt gacgatgtag ccatcgacat cgtccccctt ggtctcggtc aagccagtgg
     3961 aacagatggc gatgatcttg ggggctgcgc gtttgcggat gttcaacaag gcggcttcga
     4021 tgttgtcata gccccccaga atggaactca cctcgttcat cgccgtggtt tgcagcggaa
     4081 ttgcctcttt gaaatggcgc accagcagca ccaggccaaa cgaggtgcag ccctgcgagc
     4141 cgtgcatcac cggcatgcag gcgtcaagcc cgagaaatgc cagactcgcc ccgagtggct
     4201 ggctcatctt gagcggattc accgtgcagg cttttttgga ttcaacgacg ctggccatat
     4261 tgcattgctt tcagtagggg tggttcagac cggtagctct tcagccgaat ggtccattga
     4321 cctggcggcg actcccgtca ataagtccat cggacgcgcg ctgggcagca ccgcgcccag
     4381 ggtttcaccg tcatcacccc aaggggccgg aatgcgcacc tgctgccaga ccgggttgta
     4441 aatggcacgg tcaatctcac gcaccagctc gaccatgcct tcatatccgc cgtaggggtg
     4501 gtgacgctcc tggttgatgt cgagccaggg catgcgcgct ttcagcgcga caaactggct
     4561 gcgtccgccc gagagcatga tgtcagcccg cgcatcgcgc agcatgtcgt acatctcgcg
     4621 cggggtcatg ttgtcgatca tgtgggcatc gtcacccatg atctccttga ttttttcctt
     4681 gtcttgcctg gtagattttt tgacgctggt gccaactatc tccatgcctg cctcttgcag
     4741 cgccgccacc accgaccagg acttgacgcc gccggtgatc agcagcacgc gttttccggc
     4801 caggcgctgc ttgaagtgtt cgatgcgctg ccaggcacgc gcctcttcaa cttcaatcag
     4861 ccgctcggtg cggcccagca aatcatccgg tgccccctgc tgcaccagca ccttggcaat
     4921 ctggcgcagc gtgtcgctca tgtcgccaat gccgtagaag gaaccttcaa agtagggaat
     4981 gccccagcgc tgctccattt tggtggcgat gttgatcatc gacttggagc acaccatcat
     5041 gttgaccctg gcccggtggg cactggcaat gtcgttgtaa cgcccatcac ccgctatgca
     5101 agacagaatg cggatgccca agccgtccag caatggcttg acctgccaca gctcgcctac
     5161 caggttgtac tcgccaatga tgttgatgtc atacggcgtg gtgaactcag gctccaccgt
     5221 gccgatcacg tagtccagca gggcttcggc cccgagcttg ttgcccaggt tcttgatgcc
     5281 ggcaaaaccg ggggcattga ccgcaataat gggcttgcca aacttctcgg tggcacgttg
     5341 acacaccgct tcaatgtcat cgcccaccag cgccgtgacg caggtctggt acacaaacac
     5401 ggcaggcggg ttgtactttt ccagaatctc gcggatcgat ttgaacaggc gcttttcccc
     5461 gccataaatc acatcgagtt cattggtgtc ggtggtgaag ccactgcggt agatttgcgg
     5521 gcctgacgag g
//
LOCUS       DAOSLL010000288         5497 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13297_length_5497_cov_4.800441, whole genome shotgun sequence.
ACCESSION   DAOSLL010000288 DAOSLL010000000
VERSION     DAOSLL010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13297_length_5497_cov_4.800441"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2784)
                     /locus_tag="PLB25_12775"
     CDS             complement(<1..2784)
                     /locus_tag="PLB25_12775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE42487.1"
                     /translation="MKTSRVDANASNAIGSARTWGISTVLTCLSLLLLLPLLAFLLHW
                     LSDQGQRIQGSTESAAMNLAQISAHTLERQLSDTQQFLATMAARPNVRALDVSRCDPV
                     FGDFHKSHPGFTVLTTTTLDGKVVCGSLGNSANRPSLTAPLTTTQRAGAPQFQLGRAQ
                     KGSISGLWVLPISLPLLDDSGAAQGAIAAWINLTRLSPLNESTLNRMPTDTISTVFDD
                     NGVILARSRDADQRVGEDRTGFPQASQAIKQREGFFQTTSQADGIHRLYAVVPVEGTR
                     WVVSVGIPTAAMDTELAQDRHQGYTLFGGAVLFTGLMLWLVWHYVLRPIQRGAAIAQA
                     VKGGDLQRRIPMNRVGRISEFKSMANDFNTMLDTLVADRERLAQNEADLRTLFTEMRS
                     GFAVHDLICDEAGTPVDYRFVAVNPAFEAMTGLRADKLIGKRVLEVLPTTEPVWIERY
                     GHVALTGEATIFESYSAALNKYFDVSAFRTRPGQFGVMTLDITERRLAEMALTRERAL
                     LRRVIDTIPDLIFFKDVQGRYLGCNKAFEAFAGRTEQAQAGKTDFDFFDHETASFFRE
                     QDQKMLQSGDVRQNEEWVNYPDGHQVLLQTIKTPFTDASGESIGVLGISRDITALQQA
                     QVEIKASNETYQAILATALDGFWIADVQGQLLEVNNAYAAMSGYSRDELRGMYIFDLD
                     AEESPEVVLRRSQRLQSSGSDLFQTRHRRKNGELWDAEVSAYYWKKQNRHFVFIRDIT
                     QRKQAEGQLQQAAAVFENTQEGMMVTDLQDTIVRVNPAFTALTGFSAEEAIGKKPSIL
                     NSPNQDVSLFVLMRRNLARHGHWKGEIINRRKDGTDFSQWMTISAILDSANQISHYVS
                     TFTDISQLKEAQAKIHSLAFFDPLTQLPNRRLLLDRLHRAVALCARRKSCGAVLLIDL
                     DNFKLLNDTEGHEA"
     gene            complement(2781..>5497)
                     /locus_tag="PLB25_12780"
     CDS             complement(2781..>5497)
                     /locus_tag="PLB25_12780"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE42488.1"
                     /translation="AATDKSRIMEQLPPRGFNITLDRFLDGYDGAAILVNPRKVEVLV
                     SDKGMPDVGWIVAAVLPTKEAFAPIRAMQRRLLLATLLLTLLAGALTWWMLRRQLAPM
                     LTTVQTLAKLADNQTVPQALAIARQDEVGDMVAGFNQLLKTLAQHETALSESANRYCQ
                     LVDDLAIGLVIHSPTGEVIMSNQLALELLGVTEDQLLGKTSLDPSWDVIHEDGAPFTG
                     QTHPAPQAIATGQAIENVVMGVFRTTTQSRVWLLVTAKPQWHSDGSLQQVVVTFSDIS
                     QRKQAEAALAKSETFKNTILNSLTAQIAVVDASGVIQAVNDTWRRFAQDNSSEPDQAA
                     RLTGIGSNYLAACEDSQPAQNQDAVDARSGIQAVLDGRLPSFSLEYRCNSPQQKRWFS
                     MTVVPLGQDVRDGAVITHTDITQLKQITESKQHRSHILEMLANDQPVATILDALVRGV
                     ERLQLNAIASVLLLSSDGRHLERAIAPSLPVFFIDAFEGIEIGMGVGACGTSAFTGKR
                     VVVEDIASHPFWLPYKELAARAGLASCWSQPFRAANGQLLGTFAIYQPTAHEPSAYDI
                     SLIEQSAQLASITVERSLALQQLHDSEARFRSMMEDIAGVAAQGYTMDGTVTFWNHAS
                     EKLYGYSAEEALGHNLLDLIIPPEMHDGVKAAMAEMIETGQPIPAGELPLKAKDGSVV
                     PVFSSHALVKPVGGSPELFCLDIDLTERKQMEEQVRRLAFFDPLTKLPNRRLLDDRLK
                     QTMAASARTGRHGALVFLDLDNFKPLNDTHGHEVGDLLLIEVAHRLKACVREVDTVVR
                     VGGDEFVVMLGELKTDRDESALQANLIAEKIRLTLAEPYALTVRHEGQPDQTVLHQCS
                     ASLGVALFSNHDTSQIDILKWADAAMYQAKHEGRNRVRFHDLNNAGNQ"
BASE COUNT         1056 a         1633 c         1639 g         1169 t
ORIGIN      
        1 tgcctcatgg ccttcggtgt cattgagcag cttgaagttg tccaggtcga tcagcagcac
       61 ggcaccgcaa cttttgcgcc gggcgcacag ggccaccgcc cggtgcaggc ggtcgagcag
      121 caggcggcgg ttgggcaact gggtcagcgg atcaaaaaaa gccaggctgt ggattttggc
      181 ctgtgcctcc ttgagttgcg agatgtcagt gaaggtcgag acatagtgac tgatctggtt
      241 ggccgagtcc agaatggccg aaatggtcat ccactgcgag aaatctgtgc catctttgcg
      301 gcggttgatg atttcgccct tccaatggcc atgacgcgcc aggttcctgc gcatcaatac
      361 aaaaagcgac acatcctggt tcggcgagtt cagtatcgac ggctttttgc cgatggcttc
      421 ttcggcgctg aaacccgtta gcgcggtgaa ggccgggttg acccgaacga tggtgtcttg
      481 caggtcggtc accatcatgc cctcctgagt gttctcgaag acggctgcgg cttgctgcaa
      541 ctggccttca gcttgttttc gttgggtgat atcacggatg aagacgaagt gccggttctg
      601 ctttttccag tagtaggcgc tgacctcggc atcccacaac tcgccgtttt tgcgccgatg
      661 ccgcgtctgg aaaaggtcgc tgccgcttga ctggagccgc tgactgcggc gaaggacgac
      721 ctcgggcgat tcttctgcgt ccagatcaaa gatgtacatg ccccgcaact cgtcgcgcga
      781 atagcccgac atggccgcat aggcgttgtt cacctccagc aattggccct gcacgtcggc
      841 aatccagaag ccgtcgagcg cagtggccag aatcgcctgg taggtctcat tgctggcctt
      901 gatctccacc tgcgcctgtt gcaatgcagt gatgtcacgg ctgatgccca acacgccaat
      961 ggactcgccg ctggcatcgg tgaacggcgt cttgatcgtt tgcagcagca cctgatggcc
     1021 atccgggtag ttcacccatt cttcgttttg ccgtacgtcg ccactttgca gcattttttg
     1081 gtcctgctcg cgaaagaaac tggcggtctc gtgatcaaaa aaatcaaagt cggtcttgcc
     1141 ggcctgcgcc tgctcggtcc gcccggcaaa ggcttcaaac gccttgttgc agcccaagta
     1201 gcggccctga acatccttga aaaaaatcag gtcgggaata gtgtctatca cccggcgcag
     1261 caaggctctt tcgcgcgtca gtgccatttc tgccagccga cgctcagtga tgtcgagcgt
     1321 catcacgccg aattggccgg ggcgtgtgcg aaaagcgctg acgtcaaagt acttgttcag
     1381 ggcggcactg tagctttcaa agatggtcgc ctcgccagtc agggccacat ggccatagcg
     1441 ctcgatccat accggctcgg ttgtcggcag tacctctaga acccgtttac caatcagttt
     1501 gtcggcgcgc aagccggtca tggcttcaaa cgccgggttg accgcgacaa agcggtagtc
     1561 aactggtgtg cccgcctcat cacaaatcag gtcgtgaacc gcaaaaccac tgcgcatttc
     1621 ggtaaacagg gtgcgcaagt cagcctcatt ctgtgccagc ctttcccggt cagccaccaa
     1681 ggtgtccagc atggtattga agtcattggc catgcttttg aactcgctga tgcgcccgac
     1741 cctgttcatc ggtatgcggc gttgcagatc accccctttg accgcctggg caattgccgc
     1801 accccgctgt atcggtcgca acacatagtg ccagaccagc cacaacatca acccggtaaa
     1861 caaaacggca cccccaaaca gggtgtaacc ctggtgccgg tcctgcgcca gctcggtgtc
     1921 catggcagca gtcggaatgc cgactgacac aacccagcgc gtgccttcca ccggcaccac
     1981 tgcgtagagc cggtgaatgc cgtctgcctg cgatgtggtc tgaaaaaacc cttcacgttg
     2041 cttgatcgcc tgcgaagcct ggggaaaccc agtgcggtct tcgccaacac gctggtcggc
     2101 atcacgcgag cgggccaaaa tgacaccgtt gtcgtcaaac accgtgctga tggtgtctgt
     2161 gggcatacgg ttcaaggtgc tttcgttgag cgggctcaaa cgcgtcaggt tgatccaggc
     2221 ggcaatcgca ccctgcgcgg caccgctatc gtccagcagc ggcagactga ttggcagcac
     2281 ccagagtccg ctgatgctcc ccttttgcgc ccgtccaagc tgaaactgtg gcgcaccagc
     2341 gcgctgggtg gttgtcagcg gagcagtcag gctcgggcga ttggccgaat ttcccaacga
     2401 cccgcaaacc accttgccgt ccagggtggt ggtggtcagt acggtaaagc cggggtgaga
     2461 cttgtggaaa tcaccaaaca ccgggtcgca gcggctgaca tcgagcgcac gcacattggg
     2521 gcgcgcagcc attgttgcca aaaattgctg cgtgtcgctg agctggcgct ccagcgtgtg
     2581 cgccgaaatt tgcgccagat tcatggcagc cgattcggtg ctgccctgaa tgcgctggcc
     2641 ttggtcgctg agccagtgca acagaaaggc cagcaggggc aacaacagca acagcgacag
     2701 gcaggtcagc acggtgctga tgccccaggt tcgggccgac ccgatggcgt tgctggcgtt
     2761 ggcatcaacg cggctggtct tcattggtta cctgcattgt tcaagtcatg aaaccgcacc
     2821 cggttgcgcc cctcatgctt ggcctggtac atggcggcat cggcccattt caggatgtcg
     2881 atctggctgg tgtcgtggtt ggagaacagc gccacaccca ggctggctga gcattgatgc
     2941 agcacggttt ggtcgggttg gccctcgtgc cggacggtca atgcataagg ttcggccagg
     3001 gtcagtcgaa tcttctcggc aatcaggttg gcctgcaggg ccgactcgtc ccgatcggtc
     3061 ttgagctcgc cgagcatcac gacaaattcg tcgccgccga cacgtaccac cgtgtccacc
     3121 tcgcgcacgc aggccttgag ccggtgcgcc acttcgatca gcagcaggtc gcccacttca
     3181 tggccgtggg tgtcattgag cggtttgaag ttgtccagat ccagaaacac cagcgcgcca
     3241 tgccgcccgg tgcgggcact ggcggccatg gtctgcttca gccggtcatc gagcagacgc
     3301 cggtttggca gtttggtgag cgggtcaaag aaggccagtc ggcgtacctg ctcttccatt
     3361 tgcttgcgct cggtcaggtc aatgtccaga caaaataact cgggcgagcc accgacgggt
     3421 ttgaccagcg catggctgga aaacaccggc accacagagc catctttggc cttgagcggc
     3481 aactcgccag cgggtattgg ctgacccgtt tcgatcatct cggccatggc ggccttcacg
     3541 ccgtcgtgca tctcgggcgg aatgatcagg tccagcagat tgtggcctaa ggcttcctcg
     3601 gcgctgtagc catagagttt ttcggaggca tggttccaga aggtcacggt accgtccatc
     3661 gtgtagcctt gcgccgccac gccggcaatg tcttccatca tgctgcgaaa gcgtgcttcg
     3721 ctgtcgtgca gttgttgcag cgccagactt ctttcgaccg tgatgctggc caattgggcc
     3781 gactgttcga tcaggctgat gtcataagcg ctgggttcgt gcgcagtggg ctggtaaatg
     3841 gcaaatgtcc ccagcaactg gccgttggcg gcacgaaacg gctgcgacca gcacgaagcc
     3901 agcccggcgc gagcagccaa ctccttgtaa ggcagccaaa acgggtggct ggcgatgtct
     3961 tccaccacca cgcgttttcc ggtaaatgcc gatgtgccgc aagcgcccac gcccatgccg
     4021 atctcaatgc cttcaaatgc gtcaatgaaa aaaacgggca agctcggcgc aatcgcacgt
     4081 tcaaggtggc ggccgtcgct gctgagcaac aacacgctgg caattgcgtt gagttgaagt
     4141 cgctcaacac ccctcactag cgcatcaaga atagtcgcca ctggctggtc gttggccagc
     4201 atttcgagaa tgtggctgcg gtgttgcttg gactcggtga tctgcttgag ctgggtgatg
     4261 tcggtgtggg tgatgactgc accatcgcgc acgtcctggc caagtggtac cacggtcatg
     4321 gaaaaccagc gcttttgctg cggcgagttg caccggtatt ccaggctgaa actgggcagg
     4381 cgaccgtcca gcacggcctg gatgccgctg cgggcatcga ccgcatcctg gttctgcgcc
     4441 ggttggctgt cttcgcaggc agccagatag ttgctgccga tgccggtgag ccgcgcagcc
     4501 tggtcaggtt cgctgctgtt gtcctgcgca aagcgccgcc aggtgtcatt gacggcttga
     4561 atgacaccac tggcatccac caccgcaatc tgggcggtca gggaattcag gatggtgttc
     4621 ttgaaggttt cacttttcgc cagggccgct tcggcttgct tgcgctgact gatatcgctg
     4681 aaagtgacaa ccacctgttg caggctaccg tcggagtgcc attgcggctt ggcggtgacc
     4741 agcaaccaga cccggctttg cgtggtcgtc cgaaacacgc ccatcactac gttttcaatc
     4801 gcctggccgg tggctatggc ttgtggtgcc gggtgggtct gacccgtgaa gggcgcccca
     4861 tcttcatgaa tcacatccca acttgggtcc agcgaggttt tgcccaacaa ctggtcttca
     4921 gtgacaccca gcagttccag cgccaactgg ttgctcatga tgacttctcc ggttggcgaa
     4981 tggatgacaa ggccgatcgc cagatcgtcc accagttggc agtaacggtt ggcactctcg
     5041 ctcaatgcgg tttcatgctg agccagggtt ttcaaaagct ggttgaaacc ggccaccatg
     5101 tcgccaactt catcctggcg agcgatagcc agggcttgcg gtaccgtttg gttgtctgcc
     5161 agtttggcca gagtttgaac tgtggtcaac atgggtgcca gttggcggcg cagcatccac
     5221 caggtgaggg caccagccag tagcgtcagc agcagggtgg ccaacagcag gcgccgctgc
     5281 attgccctaa tcggggcaaa ggcttctttg gtcggcagca ccgccgccac gatccatccc
     5341 acatctggca tgcccttgtc agagaccagc acctccacct tgcgcgggtt gaccaagatg
     5401 gcagccccat cgtaaccgtc gagaaagcgg tcaagcgtta tgttgaaacc gcgcggcggc
     5461 agttgctcca tgatgcggga cttgtcggtg gccgcca
//
LOCUS       DAOSLL010000289         5483 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13338_length_5483_cov_4.517318, whole genome shotgun sequence.
ACCESSION   DAOSLL010000289 DAOSLL010000000
VERSION     DAOSLL010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5483)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5483)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5483
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13338_length_5483_cov_4.517318"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            238..372
                     /locus_tag="PLB25_12785"
     CDS             238..372
                     /locus_tag="PLB25_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42489.1"
                     /translation="MKFLGNLVDGLAAAQAHLTKLGFAPRRVRFALLRFTHDLWNIRM
                     "
     gene            complement(540..986)
                     /locus_tag="PLB25_12790"
     CDS             complement(540..986)
                     /locus_tag="PLB25_12790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008166344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HOE42490.1"
                     /translation="MNKASLCAKAACPCDSGQTYVDCCGLWHAHFDQGEFAPTPEALM
                     RSRYSAYVLGLIDYLLATWHPSTSPGELELSPVKWLGLEVRHAQDSGAAGVVEFVARY
                     RDAGRAVRLHEISRFVREDGRWTYIDGQMQEPEMGHAGSGTPRHNG"
     gene            1295..2197
                     /locus_tag="PLB25_12795"
     CDS             1295..2197
                     /locus_tag="PLB25_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010091345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOE42491.1"
                     /translation="MSRFATRLLTTMILLLAFGASQAQDRGATLRKIADSGSIALGFH
                     ESTPPFTYLDADGQPVGYSYDVATKIADAVKRELKLPALQIKRVPFTTQNRFPMVQNG
                     TADLSCGATTHTTEREKLVAFSNTIFIAGSRLMTRKNSGIKEFSDLTGKNVVTFAAST
                     SEKMLRKMNAEQDRRINIVTTFDRGDTPFSVLQAGHADAFMMDDVLLYTAIHQTWRPD
                     DWVVTGKPQSFEAYGCFMRRDDPKFKELVDQEIARLMKSGEARLLYQKWLQSPIPPKG
                     INYAVPFSAAMAELFNNPNDKPFD"
     gene            2306..4327
                     /locus_tag="PLB25_12800"
     CDS             2306..4327
                     /locus_tag="PLB25_12800"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE42492.1"
                     /translation="MGSKLFKKYALIFSALVGFSLLASGLLGISFSYQENKQALIRLQ
                     REKAQSAAQRIAQYLTDLEQKMIATAEPESGGLALDQRKAEIQMLRRTPAFKVIRLLE
                     PNGKEVLQVSRRTPDVVGSGRDFSDAKWFAEVKSGRPYRSQVYLLDGALYMTIAMTVG
                     PPAAGITVAEVDLEFLLDGISRIKSGESGLAYVVDEQGHLIAHPDIGLVLKHTNMAVL
                     PQVQTALRNRSETGLADVDGHDLEGRRVLTSFGLIPYLNWFVFVEQSLSEAYAPLVSQ
                     AVQSALLVLVGMVLTVLATVVLVRKMVSPIQALRDGAKRIGQGVFNQHIVLRTGDELE
                     ELGDEFNRMADQLHDSYTSLEQKVEQRTRALLQSEQRLREAQQIAGLGSYVFDLSANA
                     WTSSDVLNTLLGIDENYQHTAQGWTALLHPEDRKLLVRYFNQDILGGGQTFDKVFRII
                     RQSDQAERWVHGLGKVSCDAQGRVRKVQGTIQDITERKQMEDQIRQLAFYDALTALPN
                     RRLLSDRLGQCLIGSQRNGFFGALMFLDLDNFKQINDLHGHAAGDSLLVEVAVRLKAC
                     VRKADTVARFGGDEFVVMLCELDRDKAQSSILAQAVAEKIGSSLSEPYHLTLTAEGQQ
                     PTTIIHHGSASIGVFVFGDQTSDVNNILKNADVAMYQAKAAGRNTIRCA"
     gene            4492..5307
                     /locus_tag="PLB25_12805"
     CDS             4492..5307
                     /locus_tag="PLB25_12805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOE42493.1"
                     /translation="MDTGKVTTWIEQAQPILVAFGLKALGAIAVFVVGRWLISIVTTL
                     VGAGMTRQKLDPTVQRYLVSFITVSLNIILVVAILGYFGVETTSFAALVAGAGVAIGA
                     AWSGLLGNFASGIFLLVLRPYQVGDYVMVAGVEGTVLELGLFGTTLNTPDNVKTIVGN
                     GKVMGGDIKNFSANAHRRVELVAQLAGSADVNKAIALLKEAVSNVPNIVKSPAVDVEI
                     LEFNEFGPKLAVRPHCHTEHYWQVYFDTNKAIADTLGAAGFPVATRPIKVYPV"
BASE COUNT         1171 a         1550 c         1586 g         1176 t
ORIGIN      
        1 agcaattggt agccaggtcg tctttcatgt cgatccagtc ctggcgcttg atgaggggct
       61 cagcacggac ctttttttga gccaattgac cgccaatttg aggcagccat tttcgctttg
      121 cagccgatca ggttctctgt ttgtatcaat tgccttggga ccacgctttg gctcgaacag
      181 tggcttgacc cgttcctggg tagctttctt ccaaaggcca accggcatcg gattgacatg
      241 aaattccttg gcaatctggt tgatggtctt gccgctgcgc aggcccactt aaccaagctc
      301 ggctttgcgc cgcggcgtgt gcgttttgcg cttcttcgtt tcacgcatga tctctggaac
      361 atccggatgt gaagacatcg catgtttttg tctcaagtcc gggagccact ttggcagaca
      421 cgttcagcgt ggtcagcact atgccaggct cggggcctga tctatacatc aattttgcct
      481 gcagccctta tgtggattgc gctgttagct actgatatta ttgcaaacgg tggcacggat
      541 caaccgttgt ggcgaggtgt gccactgcca gcatgaccca tttcaggttc ttgcatctgc
      601 ccatcaatat aggtccagcg cccgtcctcg cggacaaagc ggctgatctc atgcagacgc
      661 acagcgcgcc cggcatcgcg gtaccgtgcg acaaactcca ccaccccggc agcgccgctg
      721 tcctgcgcgt ggcgcacttc cagccccaac catttcactg gcgacagttc aagttcaccc
      781 ggcgaggtcg agggatgcca ggtggccagc aaatagtcaa tcaggcccag cacataggcg
      841 ctgtagcgcg aacgcatcag tgcttcaggg gtcggggcaa actcaccttg atcaaaatgt
      901 gcgtgccaca agccacagca gtcaacatag gtctgcccac tgtcacaggg acaggcggcc
      961 ttggcgcaga ggcttgcttt gttcatgcac gcgatggtaa cggcactgcg gccgaccggt
     1021 ggttcagacg gttccacgtc cagcgtctgg tagtaacccc ttccaccgta aatctggcgt
     1081 ctggcacctg accagcgcac atcagcgccg accttgccga tgtgcttcgg cgcaccggtt
     1141 tccagcagtt ccagttggtc tcagcagggg cacattcggg catctgcgca aattgtccgc
     1201 ttaggtttgt ttgcacttgg cgaaccggct gcgcacaatt gctaccattg ccgtttagcg
     1261 tcctgcacgc agcacaaaaa cagaggcacc caacatgtcc cgattcgcga cccgtttgct
     1321 cacaaccatg atcttgctgc tggcctttgg tgcttcgcag gcacaggaca ggggtgccac
     1381 actgcgcaag attgccgaca gcggttcaat cgcactgggc tttcatgagt ccacgccgcc
     1441 ttttacttac ctggatgccg acggtcagcc cgtcggctac tcttatgatg tggcgacaaa
     1501 aattgcggat gctgtcaaac gcgaactcaa actgccagcg ctgcagatca agcgggtgcc
     1561 gttcaccacg caaaaccgtt ttccgatggt gcaaaacggc actgctgacc tgagctgcgg
     1621 tgccaccacc cataccaccg agcgtgaaaa gctggtggcc ttttccaaca ccattttcat
     1681 cgccggctcg cgcctgatga cgcgcaagaa ctcaggcatc aaggagtttt ccgacttgac
     1741 cggcaaaaat gtggtcactt ttgcggcctc gacttctgag aagatgctgc gcaagatgaa
     1801 tgccgaacaa gaccgccgca tcaacatcgt gaccacgttc gaccgtggcg acacgccctt
     1861 ttcggtgttg caagctggcc acgccgatgc cttcatgatg gacgatgtgc tgctctacac
     1921 cgccatccac caaacctggc gccccgatga ttgggtggtg accggcaagc cccagtcgtt
     1981 tgaagcctat ggctgcttca tgcgccggga tgacccgaaa tttaaggaat tggtcgatca
     2041 ggagattgcc cggctcatga aatcaggcga agcccggctg ctttaccaaa aatggctgca
     2101 gtcgccgatt ccacccaaag gcatcaacta cgcagtgccg ttcagcgcgg caatggccga
     2161 gctgttcaac aatcccaacg acaagccgtt tgactaggga cgtggttatc agtaacttcc
     2221 acatgtggct cgtcagcttg tgaagcacgc tgcgtcgttg cacatcgcca gtatgtgagc
     2281 tgactctggc caataccgca aacccatggg cagcaagctc ttcaaaaaat atgcgttgat
     2341 cttcagcgcc ctggtcggct tttcgctgct ggcaagcggt ttgctgggca tcagcttttc
     2401 gtaccaggaa aacaagcagg ctctgatacg gctgcagcgg gaaaaggccc agtcagcggc
     2461 acagcgcatc gcccagtatt tgaccgacct ggaacagaag atgattgcca ctgccgaacc
     2521 cgaaagcggt ggcctggcac tggaccagcg caaggccgaa atccagatgc tgcgccgaac
     2581 ccccgccttc aaggtcatcc ggctgcttga accgaacggc aaagaggtct tgcaggtgtc
     2641 gcgacgcacc cccgatgtgg tcggcagcgg gcgtgatttt tcagatgcca aatggtttgc
     2701 cgaggtgaag tcgggacgac cataccgcag tcaggtttac ctgcttgatg gcgcgcttta
     2761 catgaccatt gcgatgacgg taggtccacc ggcggctggc atcaccgttg ccgaagtgga
     2821 tctggaattt ctgctggatg gcatttcacg cattaagtcg ggtgaatccg ggctagccta
     2881 tgtggtcgat gagcagggac acctgattgc tcatcctgac attggcctgg tgctcaaaca
     2941 taccaacatg gccgtgttac cacaggtgca aacggccctg cggaaccgct ccgagaccgg
     3001 tttggccgat gtcgatgggc acgatctgga aggcaggcgg gtattgactt ccttcggcct
     3061 gattccgtac ctgaactggt ttgtttttgt cgagcagtca ctgagcgagg cctacgcgcc
     3121 gctggtcagt caggcggtac aaagcgcttt gctggttttg gtcggcatgg tcctgacggt
     3181 gttggcgacc gtggtactgg tgcgcaagat ggtgtcgcca atccaggcgc tgcgcgatgg
     3241 cgccaagcgg attggccagg gtgttttcaa ccagcacatc gtgctgcgta ctggcgatga
     3301 actggaagag cttggcgatg aattcaaccg catggccgat cagttgcacg actcttacac
     3361 atcacttgaa cagaaggttg agcagcgcac acgtgcactg ttgcaaagcg aacaacgctt
     3421 gcgcgaagcg caacagatcg ccgggctagg cagctatgtg ttcgatctga gcgccaacgc
     3481 ctggaccagc tccgacgtgc tcaacacctt gcttggcatt gatgaaaact accagcacac
     3541 cgcgcagggc tggacggcac tgctgcatcc cgaggatcga aagctgctgg tcaggtattt
     3601 caaccaggac atccttggtg ggggccagac tttcgacaaa gtctttcgca tcatccgtca
     3661 aagcgaccag gccgaacgtt gggttcacgg gctgggcaag gtaagctgtg acgcacaggg
     3721 gcgggtgcgc aaggtccagg gcactatcca ggacatcacc gagcgcaagc aaatggaaga
     3781 ccagatccgc caactggcgt tttatgacgc gctgaccgca ctgcccaatc gccgcctgct
     3841 cagtgatcgc ctgggtcagt gcctgatcgg tagccagcgc aacggctttt tcggagcgct
     3901 gatgtttctg gatctggaca acttcaagca gatcaacgac ctgcatggcc acgcggcggg
     3961 tgatagtctg ttggttgaag ttgctgttcg gctcaaagcc tgcgtgcgca aggccgatac
     4021 cgtggcccgc tttggcggcg acgaatttgt cgtgatgctg tgcgagttgg accgggacaa
     4081 ggcgcagtcc agcatactgg cgcaagccgt agcggaaaaa atcggcagca gcttgtctga
     4141 accctatcac ctgacactga ccgcggaggg tcaacagccg acaacgatca ttcaccatgg
     4201 gtccgccagc attggcgttt ttgtgtttgg agaccagacc agcgatgtca acaacattct
     4261 gaaaaacgcg gacgtggcca tgtaccaggc caaggcggca gggcgcaaca caatccgctg
     4321 cgcctgaagt agtggtgcct gcagatgctg gccaggtcag gctggtagca tgcagccgct
     4381 ggtcaagcaa gccgggcaag caccggtgac agcaaccaaa tagcaaggcc gtagtggctc
     4441 aatcgctatt tggcagacag atgtaccaac caaagttgac aaggagtgaa catggatact
     4501 ggaaaagtta caacctggat tgaacaagcc caaccaatat tggtggcttt tggactcaag
     4561 gcgctgggcg cgattgcggt ttttgtggtc ggccgctggc tgatcagcat cgtgacaacg
     4621 ctggtcggcg cgggcatgac ccggcaaaaa cttgacccca ccgtgcagcg ctacctggta
     4681 agtttcatca cggtgtcgct caacatcatt ctggtggtcg ccattttggg ctactttggt
     4741 gtcgaaacga cttcgtttgc ggccctggtg gcaggtgccg gtgtggccat tggagcggcc
     4801 tggagcgggt tgctgggtaa ctttgcctcc ggcatttttc tgctggtgct gcgcccctac
     4861 caagtgggcg actatgtgat ggtggccggc gttgaaggca ccgtgctgga actgggcctg
     4921 tttggcacca cgctgaacac ccccgacaac gtcaaaacca ttgttggcaa cggcaaggtg
     4981 atgggtggtg acatcaagaa cttttcggcc aacgcccatc ggcgggtcga actggtcgcg
     5041 caactggcag gcagtgccga tgtcaacaag gcaattgcgt tgctgaaaga ggccgtatcg
     5101 aatgttccca acatcgtcaa gtcacctgcc gtagacgttg agattctgga gttcaacgaa
     5161 ttcgggccca aactggcggt acgtccgcat tgccataccg agcactattg gcaggtgtat
     5221 ttcgacacca acaaggccat tgccgatacg ctcggtgccg ctggcttccc ggtggcaaca
     5281 cgaccaatca aggtttatcc ggtttaaccg gtccgaaatc tgtggccgct gccgcacgaa
     5341 agaagcagag atcgcctgaa gcagttgaat ttccggctgg cagaaggcac aatatggagg
     5401 ctggaagcat gcgcctggcg cggctttcgg ggctgcatgg caatggcgca gctccaacac
     5461 aggtgcgatg tggataatgc ggt
//
LOCUS       DAOSLL010000290         5463 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13397_length_5463_cov_4.494268, whole genome shotgun sequence.
ACCESSION   DAOSLL010000290 DAOSLL010000000
VERSION     DAOSLL010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5463)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13397_length_5463_cov_4.494268"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="PLB25_12810"
     CDS             <1..918
                     /locus_tag="PLB25_12810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE42494.1"
                     /translation="TPYVARWFPKSQQGFAMGIYGAGNSGSAVNKFLAPALVVGFGWT
                     MVPQVYAGIMLGTVLLFWFFSHSDPGHLVPSHVTFRDQLKSLKDPKVIKYCQYYSIVF
                     GGYVALSLWMVQYYVGEFGLDIRTAALLAACFSLPGGVLRAVGGWLSDKYGAHAMTWW
                     VLWVSWVCLFLLSYPQTDFTITTITGPKTFHIGLNVYLFTILMFTLGIAWAFGKASVF
                     KYISDDYPHNIGTISGIVGLAGGMGGFLLPIMFGALMDLTGIRSSAFMLMYGVVWVSL
                     IWMYWTEVRAKEYLAHQSPPELQAQFSSN"
     gene            927..2555
                     /locus_tag="PLB25_12815"
     CDS             927..2555
                     /locus_tag="PLB25_12815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE42495.1"
                     /translation="MNQTTTSRADIADWRPEDTQFWETTGKKIAYRNLWISIPSLLCG
                     FAIWGMWGIITVQMLNLGFPFSQAELFTLTAISGLAGATMRIPASFLIRVAGGRNTIF
                     LTTAMLLAPAIGTGLALQHKEWPLWVFQLMALWSGVGGGNFASSMSNISTFFPKRLQG
                     TALGLNAGLGNFGVTTMQIVIPLVMTLSLFGSFGGESMTLVKDSGWILGKIAAGTPTW
                     IQNAGFAWLLSLIPLSALALWGMNNLSTVSPNIGGTLSAFLKIIWIYTLSFVPAGLGL
                     YLYLPQPTGLGLLNMWVAMPLIIFSTLMVMKFTAFGTMKDSISKQFAIFGNKHTWSLT
                     ALYIVTFGSFIGFSMALPLAITVIFGISHVPDAAGVMQHTLKNPNAVSALTYAWIGPF
                     VGALVRPIGGWISDKMGGSIVTQVISAVMVAASGAVGYVMMQAYASATPEQYFQIFMW
                     LFVLLFAASGIGNGSTFRTIGVIFDRQQAGPVLGWTSAVAAYGAFIAPVVIGAQIKAG
                     TPQTAMYGFAIFYALCLVLNWWFYLRAGSEIKNP"
     gene            complement(2843..3136)
                     /locus_tag="PLB25_12820"
     CDS             complement(2843..3136)
                     /locus_tag="PLB25_12820"
                     /inference="COORDINATES: protein motif:HMM:NF014043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HOE42496.1"
                     /translation="MSHSEALRVIVYLDHIVQAIDRIHRYVDDMTELTFLGDEKTQDA
                     VVRNFEILGEAAHNLEVFHAEFAATHPQVPWALMYTMRNRVAHGYFKVDFELV"
     gene            complement(3133..3423)
                     /locus_tag="PLB25_12825"
     CDS             complement(3133..3423)
                     /locus_tag="PLB25_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOE42497.1"
                     /translation="MRPSEALQAHREAIRSIALRHRVTNVRVFGSVVHGDDTEESDLD
                     LLVEPTGETTMMDIAKIQLELAQLLKVAVDVLTPKALPPKFLEQVIAEAQAL"
     gene            complement(3566..3925)
                     /locus_tag="PLB25_12830"
     CDS             complement(3566..3925)
                     /locus_tag="PLB25_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42498.1"
                     /translation="MLAWVKENPAVRSPLVAAEVDIHRTDEQGNLAWSPIAASLLEIS
                     PNRQDVLDGFAKHFRPMCWSGSIANVLQPYQGLLTQLGKGVDAGIAMWAKEQLAQMNQ
                     RISQERWREQTTDERFE"
     gene            4513..>5463
                     /gene="coaBC"
                     /locus_tag="PLB25_12835"
     CDS             4513..>5463
                     /gene="coaBC"
                     /locus_tag="PLB25_12835"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HOE42499.1"
                     /translation="MKDLAGKHIVLGLTGGIACYKAAELCRLLIKAGATVQVVMTAAA
                     EQFITPVTMQALSQHVVYGSQWDARVANNMAHINLSREADALLLAPCSADFIARLAQG
                     QADDLLSLMCLARPAQAVPLLIAPAMNREMWAHPATRRNLAQVAADGATVLGVGAGDQ
                     ACGENGDGRMLEPHELLEDLIAFFQPKLLAGQPVLITAGPTYEAIDPVRGITNLSSGK
                     MGFAIARAAREAGAAVTLVAGPVSLPTPRGVLRVDVRSASNMLSAVMEHAQAARIFVA
                     TAAVADWRPEQAADQKMKKEGSGLPPALRFVENADILATLA"
BASE COUNT         1044 a         1557 c         1604 g         1258 t
ORIGIN      
        1 accccctacg tggcccgctg gttcccgaaa tcgcagcaag gttttgccat gggcatttat
       61 ggtgcgggca actccgggtc ggcggtgaac aagttcctgg cacccgcgct ggtggtgggt
      121 tttggctgga ccatggtgcc gcaagtctat gccggcatca tgctgggcac ggtgctgctg
      181 ttctggttct tcagccacag cgaccccggc catctggtgc ccagccacgt caccttccgc
      241 gaccagctca agtctttgaa agaccccaaa gtcatcaagt actgccagta ctacagcatt
      301 gtgtttggcg gctacgtggc tttgtcgctg tggatggtgc agtactacgt gggcgaattt
      361 ggcctggaca ttcgcaccgc ggcgctgctg gccgcgtgtt tttcattgcc cggcggcgtg
      421 ttgcgggcag tcggtggctg gttgtcggac aaatatggcg cgcatgccat gacctggtgg
      481 gtgttgtggg tgagttgggt ctgcctgttc ctgctgagtt acccgcaaac cgacttcacc
      541 atcaccacca tcaccggccc caagaccttt cacatcggcc tgaatgtcta tctgttcacc
      601 atcctgatgt tcacgctggg cattgcctgg gcatttggca aggccagtgt cttcaagtac
      661 atcagcgatg actacccgca caacatcggc accatcagcg gcatcgtcgg gctggctggt
      721 ggcatgggcg gtttcctctt gccgatcatg tttggtgccc tgatggacct gaccggcatc
      781 cgttccagtg ccttcatgct gatgtatggc gtggtctggg tgtccttgat ctggatgtac
      841 tggaccgaag tgcgggccaa ggaatacctg gcgcaccaga gcccgcccga gctgcaagcc
      901 caattttctt ccaactgaaa gactgtatga atcaaaccac aacgtcaaga gccgacattg
      961 ccgactggcg accggaagac acccagttct gggagaccac gggtaaaaag atcgcctacc
     1021 gcaacctgtg gatttcgatt cccagcctgt tatgcggctt tgccatctgg ggcatgtggg
     1081 gcatcatcac ggtgcagatg ctcaacctgg gttttccgtt ttcgcaggct gagttgttca
     1141 ccctgaccgc gatttcgggg ctggccggtg ccaccatgcg gattcccgcg tcgtttttga
     1201 tccgcgttgc cggtgggcgc aacaccattt tcttgaccac cgccatgctg ctggcaccgg
     1261 ccattggcac tggtttggca ctgcagcaca aggaatggcc gttgtgggtg ttccagttga
     1321 tggccttgtg gtcgggcgtg ggcggtggca actttgccag ttcgatgtcc aacatcagca
     1381 cctttttccc caaacggctg caaggcacgg cgctgggcct gaacgccgga ctgggcaact
     1441 ttggtgtgac caccatgcag atcgtgattc cgctggtgat gaccctgagc ctgtttggca
     1501 gctttggcgg tgagtcgatg acgctggtga aagacagtgg ctggattctg ggcaaaattg
     1561 ccgccggtac cccgacctgg attcagaacg ccggttttgc ctggctgctg tctttgattc
     1621 cgctctcagc gctggcgctg tggggcatga acaacctgag cacggtgtca cccaacattg
     1681 gtggcaccct gagtgcgttc ttgaaaatca tctggattta caccctgtcg tttgtgccgg
     1741 ccggtctggg cctgtacctg tacctgcccc aaccgaccgg gctgggttta ctcaacatgt
     1801 gggtggcaat gccgctgatc atcttcagca ccctgatggt gatgaagttc accgcctttg
     1861 gcaccatgaa ggacagcatc agcaagcagt ttgccatttt cggcaacaaa cacacctggt
     1921 cacttacagc gctgtacatc gtcacctttg gttcgttcat cggtttctcg atggccttgc
     1981 cgctggccat cacggtgatt tttggcatca gccatgtgcc cgatgcggct ggtgtgatgc
     2041 agcacacgct gaaaaacccc aacgcggtgt cggcgctgac ctatgcctgg attggcccgt
     2101 ttgtcggcgc gctggtgcgg ccgattggcg gctggatttc ggacaagatg ggcggctcaa
     2161 tcgtcaccca ggtgatctct gccgtgatgg tggcggcctc tggcgcggtg ggttatgtga
     2221 tgatgcaggc ctacgcatcg gccacaccag agcagtattt ccagatcttc atgtggttgt
     2281 ttgtgttgtt atttgcggcc agcggcattg gcaacggctc aacttttcgc accatcggcg
     2341 tcatctttga ccgccagcaa gccggcccgg tgctgggttg gacctctgcg gtggccgctt
     2401 acggtgcctt tattgcgccg gtggtgattg gtgcgcagat caaggctggc acacctcaga
     2461 ccgcgatgta cggctttgcc atcttttacg cactgtgtct ggtgctgaac tggtggtttt
     2521 acctgcgcgc cgggtcagag atcaaaaacc cctgagcgac ctgaaagcgg gtgtgccaac
     2581 cgcgcagcag cgcgacagtc agtggtgtct catcggcatt gcctcgctcg ctgttggggg
     2641 gtggccttgg tgaacagtcg caggttggcg aggtagtgtg aggcggcggt gaagcagggt
     2701 tgcctgcggg caagcgctga gtgccacgcc agcactgcaa gtgatacagc ccactgagga
     2761 acaaacaata tcaagttgct tgaggggtaa ccagcgccaa cacctgactc cgcagttctg
     2821 gcaaatcagc atgaattgtt tttcacacca attcgaaatc caccttgaaa taaccgtggg
     2881 caacgcgatt gcgcatggtg tacatcaaag cccagggaac ttgcgggtgc gtagccgcaa
     2941 attcggcatg aaaaacctct aggttgtgcg ccgcctcacc cagaatttca aaatttctga
     3001 cgactgcatc ttgcgtcttc tcatcaccaa gaaaggtgag ttcggtcatg tcatccacat
     3061 aacggtggat gcggtcaatg gcctgcacga tgtggtccag ataaactata acgcgcaagg
     3121 cttcggagtg actcacagtg cctgtgcctc ggcaattacc tgctccagaa acttgggtgg
     3181 cagggccttt ggtgtcagca catctaccgc cactttgagc aattgcgcca attccaattg
     3241 aattttggca atgtccatca tggtggtttc acctgttggc tcaaccagca ggtccaggtc
     3301 actttcttca gtgtcgtcac cgtgcaccac cgaaccgaac acccggacgt tggtcacgcg
     3361 atgacgcagg gcaatactgc ggatggcttc gcggtgggct tgcagtgcct cagagggtcg
     3421 cattcagatt tctccttggg gattggatgc ccatgatact ttttaaaact tggctcaata
     3481 cactcacaat gttctgttga tgaaaagttg ctataaggtt aagagctatc aatacttgct
     3541 gataaagggc tatcgggtaa tttgctcact cgaatcgctc atcggtcgtt tgttcacgcc
     3601 agcgttcctg gctaatccgt tgattcattt gcgcgagctg ttctttcgcc cacatcgcaa
     3661 tgcctgcgtc cacgcccttc cccagttgag tgagcaaccc ttggtagggc tgaagaacgt
     3721 tggcaatgct gccactccaa cacatcggac gaaaatgttt ggcaaagcca tctaacacgt
     3781 cctgccggtt tggcgagatt tccaacaaac tggctgctat tggcgaccat gcgaggttgc
     3841 cttgttcatc agtgcggtgg atatcaacct ccgcagccac caaaggtgac cgaactgcgg
     3901 gattctcctt gacccaagcc agcaaggtgt cgccgcgtca actgagttga cgacgctttt
     3961 gccgatcatg aaaaactgtg agacagatga acgccccatt gccgctggcg gctgtccgag
     4021 gaatgtgtgc tgctgcccac cctcttggcc acgcacaagt caagaaccgg atccaacagc
     4081 acttgacacc caacgccact gtaattgctg atggcctggc ctatttctct gcggcttgcg
     4141 ggctcgcctg caaacatgaa cctgacgttg gccaagtctc cgacgtcttt cggagcgacc
     4201 gctttcgatg aagtacttcg gcaagtgcgc tggcaattgg gtcgtgtgac cgggcttgtg
     4261 agcaagcgca cgacccagtt gccatgccgt gcgtcgccag gtggccggtt ggattgatgg
     4321 cgcaacggtt gcgagcccac cgcggcggac gctttggcac cggtgtttcg accccccaac
     4381 cgggctgcaa acctggaaat ccggcacccc acaccggggt cgattctcgc tacccgcttc
     4441 aaaggggcca gacagcctgc tggcgattgc aatcgccccg agcccgccgg gcgctctgtt
     4501 acattgccac gcatgaaaga tttagctgga aaacacattg ttttgggtct tactggcggc
     4561 attgcctgtt acaaagctgc cgagctgtgc cgcctgttga tcaaggccgg tgccaccgtg
     4621 caggtggtga tgacggccgc tgccgagcag ttcatcacgc cggtcacgat gcaggccttg
     4681 agccaacatg tggtgtacgg ttcgcagtgg gatgcccgtg ttgccaacaa catggcgcac
     4741 atcaatctga gccgcgaagc cgatgcgctg ctgctggcac cgtgcagtgc agacttcatc
     4801 gcccgtttgg cgcagggcca ggccgatgat ttgctgagcc tgatgtgtct ggcccggcca
     4861 gcacaagccg tgcccttgct gatcgccccg gccatgaacc gcgagatgtg ggcacacccg
     4921 gccacccggc gcaatctggc tcaagtggcg gctgatggtg cgaccgtgct gggggtgggt
     4981 gctggtgacc aagcctgtgg cgaaaacggc gacgggcgca tgctggagcc gcacgaactg
     5041 ctcgaagacc tgattgcctt ttttcagccc aagctgttgg ctggtcaacc agtgctgatc
     5101 accgccggcc ccacttacga ggcgattgat ccggtgcgcg gcatcaccaa cttgtccagt
     5161 ggcaagatgg gctttgcaat tgccagggca gcgcgtgagg ccggtgctgc cgtcacgctg
     5221 gtggcaggcc cggtcagcct gccaaccccg cgcggagtcc ttcgggtgga cgtgcgttca
     5281 gcatcaaata tgctctcagc cgttatggaa catgcgcagg cagctcgcat ttttgtagcg
     5341 acagcggcag tggccgactg gcgaccggag caggcggcag atcaaaagat gaaaaaagaa
     5401 gggtctggcc tgccaccagc cttgcgattt gtcgaaaacg ccgacattct ggcgacactg
     5461 gcg
//
LOCUS       DAOSLL010000291         5461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13401_length_5461_cov_4.514983, whole genome shotgun sequence.
ACCESSION   DAOSLL010000291 DAOSLL010000000
VERSION     DAOSLL010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5461
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13401_length_5461_cov_4.514983"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="PLB25_12840"
     CDS             complement(<1..196)
                     /locus_tag="PLB25_12840"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE42500.1"
                     /translation="MKFEELNLAPAIIKAVLEQGYETPTPIQAQAIPLVLAGQDLLGG
                     AQTGTGKTAAFVLPMLDKLSA"
     gene            complement(595..1266)
                     /locus_tag="PLB25_12845"
     CDS             complement(595..1266)
                     /locus_tag="PLB25_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar epimerase"
                     /protein_id="HOE42501.1"
                     /translation="MAKRYNRDALVAGASGLVGREILTLLLRDTHYQSVVSVGRRTLA
                     IEHPRLVQQIVDFANLPRLPKVDDVFIALGTTLKVAGSQAAFRSVDYDAIVAVALAAH
                     ASGATKIGVISAVGAAAGSRLFYNRVKGETEDALARLGFDSLVIARPSLLAGQRGALG
                     QPTRTGERIGQVLMQALKPLMPARYHAIEARDVAFALIESVKTDRLGTRILHSSDMQG
                     AYCGG"
     gene            1411..3078
                     /gene="ettA"
                     /locus_tag="PLB25_12850"
     CDS             1411..3078
                     /gene="ettA"
                     /locus_tag="PLB25_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HOE42502.1"
                     /translation="MAQYVFSMNRVGKIVPPKRHILKDISLSFFPGAKIGVLGTNGSG
                     KSTLLKIMAGVDKEIEGEATPMAGLNIGYLPQEPQLDPAQTVRESVEAGMGRVFSAKA
                     RLEEVYIAYGEEDADFDALAAEQHDLEAIIATSGTDTEHQLEIAADALRLPPWDAVIG
                     VLSGGEKRRVALCRLLLSHPDMLLLDEPTNHLDAESVDWLEQFLQRYPGTVVAITHDR
                     YFLDNAAEWILELDRGIGIPYKGNYSSWMEQKQARLELEHKGEESRAKTLKKELEWSR
                     QNPKARQAKSKARLARFEELSDFEYQKRNETNEIFIPVADRLGNEVIEFVNVSKSFGD
                     RLLIDNLSFKVPAGAIVGIIGPNGAGKSTLFRMISGQQQADSGTVKMGSTVKMAFVDQ
                     HRDDLANEKTVWEDVSGGLDILNIGKFQMASRAYCGRFNFNGADQQKRVGTLSGGERG
                     RLHLAKTLIAGGNVLMLDEPSNDLDVETLRALEDALLEFAGSIMVISHDRWFLDRIAT
                     HILACEGDSQWTFYNGNYHDYEEDKKKRLGEEGAKPRRMRFKALKKG"
     gene            3117..5384
                     /locus_tag="PLB25_12855"
     CDS             3117..5384
                     /locus_tag="PLB25_12855"
                     /inference="COORDINATES: protein motif:HMM:NF019410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1631 family protein"
                     /protein_id="HOE42503.1"
                     /translation="MPRSAIYQSLLEQAARAGTTMLSQVLATTRQSIREDAQRMRSLL
                     ERDQLELSVKLLDSHAMRMCERLPVILTGIFRRTVGTESKLGLLSGQALRLDQLELMD
                     EDQVQERVEMARVLQHVLLKSEAALTELNTYVCALMGFDHVVPERNPLRPESYVAALQ
                     QLMTELTVPALVRKVWLHHMAAALGEALSAAYLEWSGQLQMRRVQGVGFSVVRMPLAA
                     SGSGKGVQVRSERAIWTPQYRQTVLTLDRLRRLMAGNLDASPDTPLEAFARQFARDFE
                     SGADDGATSTACPPETGFEATLPAALDALQEMQQVDVVVQRMQQRPVPPVGRADAGMS
                     AALVREELTQSATAMSQVLSLEVVSLMVDNLVQDARLLAPVRKIIERLEPALLRLVII
                     DPRFFLDRQHPARRLLQEISARGLAFGTTEDPDFNAFLLSLQRHVSPLAVMQIDGVQP
                     FVGALQHLLQEWDDPGARATITSQIDSAVAVLGYAEQRNLLAEQMTIKLRAIPAIRQV
                     PLAMVDFLCGPWSQVMAEAQLKDTSKSDDPGQYKELVNELLWSAQPDLTRKDVARLTR
                     LVSRLLAKLREGLGLIDYPSIKTSAFFDVLMKLHQQAFRPATKEVPPKAGLAESLLTG
                     EDPWLAPAEAKASGFMAFPDEVRPHAAAPQPNQVDLLEPDLVAKSLKAGAWVELEGKT
                     GWQRLQLSWISPHNTMYLFTSARGKTQSMTQRLLERLLSQGKLRVVSDQASMLDGALD
                     AVVHTATLNSIDVKA"
BASE COUNT         1163 a         1504 c         1655 g         1139 t
ORIGIN      
        1 cagcacttaa tttatcgagc atgggcagca cgaaggcggc cgttttgccg gtgccggttt
       61 gcgcgccacc aagcagatcc tgcccggcca gcaccagcgg aattgcctgc gcctgaatcg
      121 gggtcggggt ctcgtagcct tgctcaagca cagctttgat aatggccgga gccagattta
      181 attcttcaaa tttcattaga gatgcacccc atctgagggc gccagggcat cggcctgttt
      241 tggtgcttgt gcgccaattc agtcaaaggc agatgggatc aaaagtggag ttgcaggtga
      301 ctacgactcc cagcaagttg ctggcgttgc aggcaactga agccctacaa ccggttgaat
      361 tgtcgcacgg cttgcacggc tggtcgtcgc tgaagcgaat aatgtcgttt tttcctccat
      421 ggcaagatcg atccgtcaag ttggcgtgag gcggcttgca gcggtgtcat tgtgtttcca
      481 gaccagtgcg acatcaggcg cgaagccgca gacagcgctg cagcacggca aagcgacgca
      541 acaacatgtc gggttaatag ggaaacgcaa tctgttatct ggatctgcca atagtcagcc
      601 accgcaatat gcgccctgca tgtcactcga atgcaggatg cgcgtgccca gccgatcagt
      661 cttgaccgac tcgatcaaag caaaggccac gtccctcgct tcgattgcgt ggtagcgagc
      721 tggcatcagg ggtttgagcg cctgcatcag tacctgcccg atgcgctcgc cggtacgggt
      781 tggctgtccc agcgccccgc gctggcctgc cagcaatgag ggccgagcaa tcaccaggct
      841 gtcaaagccc agccgggcca gtgcgtcttc tgtctcgccc ttcacccggt tgtaaaaaag
      901 gcgtgatcca gccgccgcac caacggccga aatcactcct attttagtag ctcctgacgc
      961 atgggcagca agggctacag ccacaatagc atcgtaatca acggatcgga aggcggcctg
     1021 gctgccggcc accttgaggg tggttcccag ggcgataaac acgtcatcga ccttgggcag
     1081 gcgtggcaaa ttggcaaagt ccacgatttg ctgaactagc ctgggatgct cgatagccaa
     1141 tgtgcgtcgg ccgacactga cgaccgactg gtaatgtgtg tcgcgtagca ataaggtgag
     1201 aatttcccgc ccgaccagcc cggatgcacc cgccaccagc gcatcccggt tgtaacgttt
     1261 cgccattgct gctccatttc gcttgccatt gccaaatttg tcacagaacg agcctgcgcc
     1321 catttcgtca tgttttgtat cagggtatgc ctgcccgagc gggtcaaacc ctgataatgg
     1381 cgatttcaca cactcgactg cacaagacct atggcccaat acgtattttc aatgaaccgt
     1441 gtcggcaaga tcgtgccgcc caagcggcat attctgaaag acatttcgct gagttttttc
     1501 ccgggtgcca agattggcgt gctgggcacg aatggttctg gcaagtctac cctgctgaaa
     1561 atcatggccg gtgttgacaa ggaaattgaa ggcgaagcaa caccaatggc ggggctgaac
     1621 attggttatt tgccgcagga gccgcagctt gacccggcgc agacggtgcg cgaaagcgtc
     1681 gaggccggca tgggccgggt gttctccgcc aaggcgcggc tggaagaggt gtacatcgcc
     1741 tacggcgaag aggatgcaga ttttgacgca ctggcggccg aacagcatga tttggaagcc
     1801 atcattgcca cctccggcac tgacaccgaa catcaactgg aaattgccgc tgatgcgctg
     1861 cgcctgccgc cgtgggatgc ggtcattggt gtgctgtcag ggggggaaaa acgccgggtt
     1921 gccctgtgcc gtctgttgct gagccatccc gacatgctgc tgcttgacga gcctaccaat
     1981 caccttgatg cagagtcggt cgattggctg gagcaatttt tgcaacgcta tccgggcacc
     2041 gtggtggcga tcacccacga ccgctacttt ctggacaatg cggccgaatg gattctggaa
     2101 ctcgaccgtg gcattggcat tccgtacaag ggcaattaca gctcgtggat ggaacaaaag
     2161 caggcgcggc tggaacttga gcacaagggc gaggaatcgc gcgccaaaac cctcaagaag
     2221 gaactggaat ggtcacgcca gaaccccaag gcccgtcagg ccaagagcaa ggcccgcttg
     2281 gcccgctttg aggaactgtc tgatttcgaa taccagaaac gcaacgaaac caacgaaatc
     2341 ttcattccgg tggccgaccg gctcggcaat gaagtcatcg agtttgtcaa tgtcagcaag
     2401 tcgtttggtg accgcctgtt gattgacaac ctgagcttca aggtgcccgc aggcgcgatt
     2461 gttggcatca ttgggcccaa tggcgctggc aaatcgacgc tgtttcgcat gatctcgggc
     2521 cagcagcagg ctgacagcgg cacggtcaag atgggttcga ccgtcaaaat ggcgtttgtt
     2581 gaccaacatc gtgatgacct tgccaacgaa aaaacagttt gggaagatgt ctcaggtggg
     2641 cttgacatcc tcaacattgg caaattccag atggccagcc gggcctattg cgggcgcttc
     2701 aacttcaatg gtgctgacca acagaagcgt gtcggcacct tgtcaggcgg cgaacgcggt
     2761 cgtctgcact tggccaagac cttgattgcc ggcggcaatg tgttgatgct cgatgagcca
     2821 tccaacgacc tcgacgtgga aaccttgcgg gcgctggaag atgccttgct ggaatttgcc
     2881 ggctccatca tggtgatcag ccatgaccgc tggttcctgg accgcattgc cacccacatt
     2941 ttggcctgcg agggcgatag ccagtggacc ttctacaacg gtaattacca cgactacgaa
     3001 gaagacaaga agaagcgcct gggtgaagag ggtgccaaac ccagacgaat gcgcttcaag
     3061 gcgctgaaaa aaggttgacc gaatggttga cactgatgac gacacctgat cgatccatgc
     3121 ctcggtctgc aatctaccag agcctgctcg aacaggcggc gcgcgcgggc acgaccatgc
     3181 tgtcacaggt attggcgact acccggcagt cgattcggga agatgcccaa cgcatgcgca
     3241 gcttgcttga gcgcgatcag cttgaactca gcgtcaaatt gctggattcg catgcaatgc
     3301 gcatgtgtga gcgtctgcca gtcattctca ctggaatttt caggcgcact gtcggcaccg
     3361 agtccaagct gggcctactg tctggtcagg ccttgcggct ggatcaactg gagctgatgg
     3421 acgaggacca ggttcaggaa cgggttgaaa tggcccgggt gttgcaacat gttttattga
     3481 agtcagaggc tgctctgacc gaactcaata cctatgtttg cgccctgatg ggctttgacc
     3541 atgtggttcc ggagcgtaat ccgctgcggc ccgagtcgta tgtggcggcc ttgcagcaac
     3601 tcatgactga gctgacggtg ccggcgctgg tgcgcaaggt gtggttgcat cacatggcag
     3661 ccgcgctcgg cgaagccttg agcgcggctt acctggagtg gtcagggcaa ttgcagatgc
     3721 gacgcgtgca gggtgtcggc ttttctgtcg ttcgcatgcc cctggcggca tcaggttcag
     3781 gcaagggcgt acaagtgcgc agcgagcgcg ctatctggac accgcagtac cgacagaccg
     3841 ttttgacgct ggaccgtttg cggcgcttga tggcgggaaa tctggatgcg tcgcccgaca
     3901 ccccgctgga ggcgtttgcc cgccagtttg cgcgtgattt tgaatccggt gcggatgacg
     3961 gtgcaacatc caccgcttgc ccgccggaaa ccggctttga ggcgacactg ccggctgcgc
     4021 tggatgcgtt gcaggaaatg cagcaagttg atgtggtggt gcaacgcatg cagcagcgtc
     4081 cggtgccgcc ggtcgggcgc gccgatgctg gcatgtctgc ggcgctggtg cgtgaggagc
     4141 tgacacaaag tgccaccgcg atgagccagg tgttgagtct tgaagtggtg tcactgatgg
     4201 tggacaacct ggtgcaagat gcgcgtttgc tggcaccggt gagaaaaatc atcgagcggc
     4261 tggagccagc cttgttgcgc ctggtgatca tcgaccctcg cttttttttg gaccgccagc
     4321 atccggcacg gcgtttgttg caggaaattt ccgcaagagg actggccttc ggtaccaccg
     4381 aagacccgga cttcaacgcc tttttgctgt ctttgcagcg gcatgtcagc ccgctggcag
     4441 tgatgcagat agacggtgtc caaccctttg tgggggcatt gcagcatctg ctacaagaat
     4501 gggacgatcc gggcgcgcga gccaccatta ccagccagat tgacagtgcg gtggcggtgt
     4561 tgggttatgc cgagcagcgc aatttgctgg ctgagcaaat gacgatcaag ttgagagcca
     4621 tcccggctat tcgacaggtg ccgctggcga tggtggattt tctgtgcgga ccctggtcgc
     4681 aggtgatggc agaagcgcaa ttgaaagaca ccagcaaatc tgacgatccg gggcagtaca
     4741 aggaactggt gaatgaactg ttgtggagtg cgcagcctga tctgacccgc aaggatgtcg
     4801 cacggctcac caggctggtt tccaggttgc tggcaaagtt gcgcgaaggc ctcggtctga
     4861 ttgactaccc atcgatcaag accagcgctt tttttgatgt cttgatgaag ttgcatcagc
     4921 aggcattccg gcctgccacc aaggaggtgc cgcccaaggc aggcctggca gaaagcctgc
     4981 tgacgggcga agatccatgg ctggcgccag ccgaagccaa ggcatccggg tttatggcat
     5041 ttccggatga agtccgtccg cacgcagcgg ccccacagcc aaatcaagtt gacctgctcg
     5101 aacctgattt ggtggcaaag tcgcttaaag cgggggcctg ggtcgaactt gagggtaaaa
     5161 cgggctggca gcgcctgcag ctttcatgga tcagcccgca caacaccatg tacctgttta
     5221 ccagcgcgcg aggcaagaca cagtcgatga cgcaacgtct gcttgagcga ctgctttcgc
     5281 aaggcaaact gcgggtcgtg tccgatcagg cttccatgct tgatggcgcg cttgatgcgg
     5341 tggttcacac cgccacgctc aacagcattg atgtcaaggc ttagccagcc gttggcgcac
     5401 cccgtcaatg tggctgcgca gcgcgtacag ctcgtcggca taagacaaag gcacggttat
     5461 c
//
LOCUS       DAOSLL010000292         5444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13455_length_5444_cov_3.889404, whole genome shotgun sequence.
ACCESSION   DAOSLL010000292 DAOSLL010000000
VERSION     DAOSLL010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13455_length_5444_cov_3.889404"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            234..647
                     /locus_tag="PLB25_12860"
     CDS             234..647
                     /locus_tag="PLB25_12860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42504.1"
                     /translation="MPRLLRTDFPHIAIPGSRTAFDAMAKTGAVLVQLHLLEYPDATK
                     ISASSAGGTWPAGQFGINSARVIAVKAGIQTALDSRLRGNDGQGLLAHDTQGTLVHDG
                     QGALAAQTPTDYNNQSCQRNSHRRYKPICHKSMLV"
     gene            733..1236
                     /locus_tag="PLB25_12865"
     CDS             733..1236
                     /locus_tag="PLB25_12865"
                     /inference="COORDINATES: protein motif:HMM:NF037202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42505.1"
                     /translation="MVGKVLINASSGFANVHKSIWQYTNGGYQVLHKWLDDRRKAGRS
                     LSQDGITHCLRVYAALQATQPMMLQVDEAILANGGWPGAFSQSHPPPDAATLAAEQMA
                     QKDQLKAQKEAAIASKNVAATASSTGVTSQFDFDDVLDALAQASGAPPRPKSSATPTA
                     KAADGIG"
     gene            1427..2806
                     /locus_tag="PLB25_12870"
     CDS             1427..2806
                     /locus_tag="PLB25_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HOE42506.1"
                     /translation="MPKTLIEPFRIKSIEPIRMTSRAEREQLLEAAKLNVFKLRAEDV
                     LIDWLTDSGTGAMSSRQWGAIMEGDESYAGARSFYRLEAVIQNITGMKYFVPTHQGRA
                     AEKVLFTAVCKKGDVVPNNCHFDTTRANLEYLGIEALDLVIAEGLQPSLIHPFKGNID
                     LERAEKLLQEQGERIPFGMITVTNNTGGGQPVAMANIRAYAALLKKYGKPFIMDVCRF
                     SENAMFIKMREPGYENTPIKTIVQEMFSYADGATISAKKDGMVNIGGFIVLRSEEWMD
                     AVRNVLIMTEGFPTYGGLAGRDLEALAVGLEEGMQEDYLRYRLRTAEYLGERLEAAGV
                     GFVKPTGGHAVYIDARTVLPNMPIAHYPAWALCNALYLEGGIRGVEIGSVMFGKRLPD
                     GVETYHSMELVRLAFPRRMYTQSHFDYAAEVIDEVKQKAASIRGVRITKQPQFLRHFT
                     CECAWADAP"
     gene            2852..3199
                     /locus_tag="PLB25_12875"
     CDS             2852..3199
                     /locus_tag="PLB25_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42507.1"
                     /translation="MHFQSHEAYFATQPTELRRRLEDIQQQVEAQVPGATKTISYNLP
                     AFRQQRTFFYFAAFKNHIGIYPPVTQDLELIEATRALRGPKGNLSFPHNAELPLPLIG
                     RIARALALQYGAA"
     gene            complement(3734..5107)
                     /locus_tag="PLB25_12880"
     CDS             complement(3734..5107)
                     /locus_tag="PLB25_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HOE42508.1"
                     /translation="MASILVIEDEQILAKNFARYFEKMGHAVAVEHDGAAGLQTALQL
                     QPDVVIVDFQLPGMDGLEIIRALRKTDDPPRIVMITGHASVTLAVDAMKAGSMDLLTK
                     PVTLASLQEVVQRALTERGERRALDLYRKRDASDASLDALIGQSLAMQNLRELIRTIA
                     AAEPSDHSPAAPILILGETGTGKELVARACHQTGPRAKAPFVEINCAALPTHLIEGEL
                     FGFEKGAFTDAHVRKIGLFEAADGGTLFLDEIGELDLILQAKLLRVLENYRVRRLGAL
                     TDRQINVRIVAATNRDLEEQVQAGRFRADLMYRLKVFQVSLPPLRARAGDVVMLARHF
                     LAQLGARYGRGQMTLDETAMAALEAHHWPGNVRELRNLLERAVLLQRSPVLGVADLAL
                     PALHASVASTSATAAREPASLEAVERQHLLRALEECNWNVTHAARKLQISRDTLRYRM
                     ERHRLQR"
     gene            complement(5112..>5444)
                     /locus_tag="PLB25_12885"
     CDS             complement(5112..>5444)
                     /locus_tag="PLB25_12885"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE42509.1"
                     /translation="MLRQILVSILANAIEVMPQGGTLAVSWSVERAALKLIIADSGPG
                     IGDEVRRALFRPFFSTKSGGLGIGLALVKRMVEQWNGQIALYPAPQRGTCVELTLPLA
                     AMVSRTPG"
BASE COUNT         1154 a         1649 c         1583 g         1058 t
ORIGIN      
        1 tcgcgtgctc aatggtctgg tgccaaaagg ccgcgccacc ggcggcggcc atcagactgg
       61 gccgcgcacg acctgctgtt tgttttcagc tttggtgaag gcaacgctcc aatccgctct
      121 caatacagat ccagtcgact accgccccga agaccccacc gccccgctgc acgccgagaa
      181 aatcttccac taccgctacg ccgtgctgca cagcccggcc taccgccagc gctatgccgc
      241 gtttgttgcg caccgacttc ccgcacatcg cgatccccgg cagccgcacg gcgttcgacg
      301 cgatggccaa aacgggggcc gtgctggtgc aattgcattt gctggagtac ccggatgcta
      361 caaaaataag tgcttctagc gcaggtggga catggcctgc aggccagttt ggcataaatt
      421 ctgcgcgcgt cattgccgtg aaggcgggaa tccagactgc actagattcc cgcctgcgcg
      481 ggaatgacgg gcaaggcctg ctggcgcatg acacgcaggg cacgctggtg catgacggac
      541 agggcgcgct ggccgcacaa acgcccacgg actacaacaa tcagagctgc cagcgcaaca
      601 gtcacaggcg ttacaagcca atttgccata aatccatgct ggtttgacag tgatttcgcg
      661 ctgcacaagt tggccgaaaa gtcgcgccaa ctggcaggta ctagcccttc gtcagacgca
      721 aggagaacgg acatggtggg caaggtgctc atcaacgcca gcagtggttt tgccaatgtg
      781 cacaagtcca tctggcagta caccaatggc ggctaccagg ttctgcacaa gtggctggac
      841 gaccggcgca aggcgggccg atccttgagc caggacggca tcacccactg cctgcgcgtg
      901 tatgccgcgc tgcaagccac gcaaccgatg atgctgcagg tggacgaggc gattcttgcc
      961 aacggtggct ggcccggcgc gttcagccaa agccacccgc cacctgatgc tgccacgctg
     1021 gccgccgagc agatggcgca aaaggatcaa ctcaaggcgc agaaggaggc cgcaattgct
     1081 tctaaaaatg tagctgctac cgcaagctcc acgggggtta caagccaatt tgactttgat
     1141 gatgtgctgg atgcgttggc tcaagcgtca ggagcgcccc cgcgcccaaa atccagtgcc
     1201 acccctaccg ccaaggcagc ggatggtatc ggatgaaccg aatcacctga cggcttggat
     1261 ccgatttttg actctcaaag tcgtactcag gctaacgctg ccaggcggca gcttgctggc
     1321 tgcgggcaca agaaaaatca tttttgctgg ttgttgcaaa tacccgacac cacgcacttt
     1381 tcaccgatgc taacttcgcg ccacccttaa ttttgacgag agaaccatgc ccaaaaccct
     1441 gatcgagccg tttcgcatca aaagcattga acccatccgc atgaccagcc gggccgaacg
     1501 cgaacagttg cttgaagccg ccaaactcaa tgtcttcaag ctgcgcgccg aagacgtgct
     1561 gattgactgg ctgaccgact ccggcaccgg ggccatgtcg agccgccagt ggggtgccat
     1621 catggaaggt gacgagagct acgccggtgc gcgcagcttt tatcggctcg aagcggtcat
     1681 ccagaacatc accggcatga agtattttgt gccaacgcac cagggccgcg ccgctgaaaa
     1741 ggtgcttttt acggcggtct gcaagaaggg cgacgtggta cccaacaact gtcactttga
     1801 caccacccgc gccaatctgg aatatctggg catcgaggcg ctggacctgg tgattgccga
     1861 aggcctgcag ccttccctga ttcacccgtt caagggcaat atcgatctgg agcgggcaga
     1921 aaagctgttg caagaacagg gcgagcgcat tccgtttggc atgatcaccg tcaccaacaa
     1981 caccggtggc ggccagccgg tggcgatggc caacatccgc gcctatgcag cgctactgaa
     2041 aaagtatggc aagcccttca tcatggacgt ttgccgtttc tcggaaaacg ccatgttcat
     2101 caagatgcgc gagccgggtt atgagaacac gcccatcaaa accatcgtac aggagatgtt
     2161 ttcttatgcc gacggtgcca ccataagcgc caaaaaagac ggcatggtca acattggcgg
     2221 ctttatcgtg ctcagaagcg aagaatggat ggacgcggtg cgcaatgtgc tgatcatgac
     2281 cgagggcttt cccacttacg gtggcctggc cggacgtgac ctggaagcgc tggcggtcgg
     2341 gctcgaagaa ggcatgcagg aagattacct gcgctaccgc ctgcgcaccg ccgaatacct
     2401 gggcgagcgg cttgaagctg ccggtgtcgg ttttgtcaag cccaccggcg ggcatgcggt
     2461 ttacatcgat gcccgcaccg tgctgccaaa catgccgatt gcgcattacc cggcctgggc
     2521 cttgtgcaat gccttgtacc tggaaggcgg cattcgcggg gtcgagattg gctcggtgat
     2581 gtttggcaag cgcctgcccg atggcgtcga gacttatcac agcatggagc tggtgcggct
     2641 ggctttcccc aggcggatgt acacgcaaag ccattttgac tatgcggccg aggtgattga
     2701 cgaggtcaag caaaaggctg ccagcattcg cggtgtgaga attaccaagc agccgcagtt
     2761 tctgcggcat ttcacctgcg agtgcgcctg ggcggatgcg ccatgaccgg ccagcggcag
     2821 gttttgacag ggagccccgg agaaaatcga catgcacttc caatctcacg aagcgtactt
     2881 cgccactcag ccgactgagc ttcgcaggcg acttgaagac atccagcagc aggtggaagc
     2941 ccaggtgcca ggcgcaacca agaccatcag ctacaacctg ccggcgttca ggcagcagcg
     3001 aacgttcttc tatttcgccg cgttcaagaa tcacatcggc atctatcctc cggtcaccca
     3061 ggaccttgaa ctcattgagg cgacccgggc cttgcgcggc ccaaagggta atctgtcatt
     3121 tccccacaat gcagagttgc cgctgccact catcggcaga atcgcccggg cgcttgcgtt
     3181 gcagtacggt gctgcttgaa atcaccttct agttcagtgc cgcgagtgct gcgcaccgcg
     3241 cgccaacgac gggccaccaa gcctttgtca caggcgcggc gcacgttttt ttgcggtcag
     3301 agcctggcat cccaccgtcg gtgtttgacc ggctcgcacg atgcgcgttg acccattttt
     3361 ttgccaacaa atgaaaaatc aggttagctt catgtcgcat ttgtgcacag gaagacaagt
     3421 gcctcagtct cttcgatgca acttgtccag agtttttcgc gctaagagag catcgtgacg
     3481 atgcgagctt ccttgataca agtccgacca atttccagtc tgatttcatt gcaggcggcg
     3541 aaacgctatt taattagtag ctaccagcgc agttagcaca agcgctggaa ggctgttgga
     3601 ggagcttcgg tcgctcgcca caacggtgat gatgattgtg cgtggtgcag tacagtgccc
     3661 gtctcctgag gtcaattccg ttgcagacag ttgacagcag cagtcgcccc cgcaaaagga
     3721 tcaggcaccg gcatcagcgt tgcagtcgat ggcgttccat tcggtagcgc agggtgtcgc
     3781 ggctaatctg cagcttgcgt gcggcgtggg tgacgttcca gttgcattct tcaagtgcgc
     3841 gcagcaggtg ctgacgctca accgcttcca gcgaggctgg ctcacgagcg gcagtggcgc
     3901 tggtggaagc gactgacgca tgcagcgcag gcagggccag atcggccacc cccagcactg
     3961 gcgagcgctg cagcaacacg gcacgctcca gcagattacg caactcgcgc acattgcccg
     4021 gccaatggtg tgcctccaac gcggccatcg cggtttcgtc cagggtcatc tggcctcggc
     4081 catagcgagc gcccaactgg gccaaaaagt gccgtgccag catgaccaca tcaccggctc
     4141 gggcacgcag cggtggcaaa ctgacctgaa aaaccttaag acggtacatc aggtcagccc
     4201 ggaaacgccc cgcctgaacc tgttcctcca ggtcccggtt ggtggctgcg acgatgcgca
     4261 cattgatttg ccgatccgtc agggcaccga gtcggcgaac ccgatagttt tccagcaccc
     4321 gcagcagttt ggcctgcagg atcaggtcaa gttcgccgat ttcatccaga aacagggtgc
     4381 cgccatcggc agcctcaaac aggccgatct tgcggacgtg agcgtcggtg aatgcgcctt
     4441 tttcaaaacc aaacaactca ccttcaatca agtgcgtggg cagtgccgca cagttgattt
     4501 caacaaacgg ggctttggcg cggggtccag tctgatggca ggcgcgcgcc accagttcct
     4561 tgccagtacc ggtttcgccc agaatcagga tcggtgccgc cggcgaatgg tctgaaggct
     4621 cggcagcggc gatggtgcga atcagctcac gcaagttctg catggccagc gactgtccga
     4681 tcagggcatc caggctggca tcgcttgcgt cgcgcttgcg atacaggtca agcgctcggc
     4741 gctcaccccg ttcggtcagg gcgcgctgca ccacctcctg caggctggcc agagtgacgg
     4801 gtttggtcag caggtccatc gacccggcct tcatagcatc gaccgccagg gtcacgctgg
     4861 cgtggccggt gatcatgacg atgcgcggcg gatcgtcggt tttgcgcagt gccctgatga
     4921 tttcaagccc gtccatgccg gggagctgaa agtcaacaat caccacatct ggctgcagtt
     4981 gcaaggcggt ctgcagaccg gcggcaccgt cgtgttcgac cgccacggcg tgacccatct
     5041 tttcaaaata gcgggcgaaa ttcttcgcca gtatctgctc gtcttcgata accagaatgc
     5101 ttgccatggt cttatcccgg cgtcctggac accattgctg ccagcggcag tgtcaattcc
     5161 acgcaggttc cgcgctgcgg cgccgggtag agcgcaattt gcccgttcca ttgctcaacc
     5221 atgcgtttaa ccagagccag gccgatgccc agaccgccac ttttggtact gaaaaagggt
     5281 cggaacaggg cacgcctgac ctcgtccccg atgccgggtc cgctgtcggc aatgatcagt
     5341 ttgagagctg cacgctccac cgaccacgaa accgccagag tgccaccctg gggcatcact
     5401 tcaatggcgt tggccaggat actgaccagg atttgccgca gcat
//
LOCUS       DAOSLL010000293         5433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13490_length_5433_cov_4.329676, whole genome shotgun sequence.
ACCESSION   DAOSLL010000293 DAOSLL010000000
VERSION     DAOSLL010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13490_length_5433_cov_4.329676"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="PLB25_12890"
     CDS             complement(<1..1295)
                     /locus_tag="PLB25_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HOE42510.1"
                     /translation="MGHKLKITGWISLGVMAGVLTTVSLQTVARGTLAPLPLEELQQL
                     AAVFGMVKSNYVEPVDEKKLITDAIAGMVAGLDPHSVYLDKKALKDFNEGTTGKFVGV
                     GIEISQEDGLVKVVSPIEGSPAFRAGLKPNDLIVKIDDTPVKGLTLNEAVKRMRGEPN
                     TKVLLSIYRKDESRTFPVTIVREEIRTQSVRSKVIEPGYAWIRLSQFQERTVADFARK
                     MEEIYKQEPKLKGLVLDLRNDPGGYLDAAVAISAAFLPENVTVVSQSGQLAESKFTYK
                     ASPRFYARRGPDPLKQLEEVTHGALKSVALVVLVNEGSASASEIVAGALQDHRRAKLL
                     GSQTFGKGSVQTAVCLDSAFRMDNCPTAALKLTTSRYYTPSGKSIQAKGIVPDVMIDE
                     TEEGNLFAALKLREADFDKHLSSGQGEEIKDPVREKARE"
     gene            1716..2123
                     /locus_tag="PLB25_12895"
     CDS             1716..2123
                     /locus_tag="PLB25_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOE42511.1"
                     /translation="MKFFLDNWMLIAVALSAGGMLLWPMISSGGPGALTADGAVQLIN
                     REKAVVIDVCEPTEYASGHVSGAKNIPLGQLEGKLASTVKNKALPVILVCLSGARSKR
                     AVSIAKKLGFENAVSLSGGMNAWRSANLPVEKA"
     gene            2141..2401
                     /gene="grxC"
                     /locus_tag="PLB25_12900"
     CDS             2141..2401
                     /gene="grxC"
                     /locus_tag="PLB25_12900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HOE42512.1"
                     /translation="MSPVKMYTTAVCPYCIRAKQLLKARGVEHIDEIRIDLHPDEVQE
                     MMRLTGRRTVPQIFIGNTHVGGCDDLIALDGQGGLLPLLSNA"
     gene            2479..2946
                     /gene="secB"
                     /locus_tag="PLB25_12905"
     CDS             2479..2946
                     /gene="secB"
                     /locus_tag="PLB25_12905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HOE42513.1"
                     /translation="MSESQDPVFQIQRVYLKEASLEQPNSPAILLEQEQPTVDIQLGV
                     NASPVAEGIYEVAVTATVHTKIKDKTVFLVEITQAGIFEVRNLPQEQMGPVMGIACPQ
                     IVYPYLRGNVADLIQRAGFPPVHLSEINFQAMYEQQQQQQAAPAEGAPRIVTQ"
     gene            2997..4022
                     /locus_tag="PLB25_12910"
     CDS             2997..4022
                     /locus_tag="PLB25_12910"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HOE42514.1"
                     /translation="MKIVVLGAGAWGTALAVSAVGQQLAQHQVTLWARSPLQTEHMLA
                     QRENQRYLAGVPLPASLTLSAAPLPQLATLAQHADLVVVATPMAALRQTLSLLRDCRA
                     PVAWLCKGFELPVSEGFGLLAHEIQAQVAPCLIAGVFSGPSFAQEVARGQPTALVAAS
                     PHESVRQALVEAFHGRSLRVYANDDMVGVEVGGAVKNVLAIGTGLCDGLALGQNARAA
                     LITRGLAEMLRLGIALGARAETFMGLSGLGDLVLTATGDLSRNRQVGLALAHGRTLAQ
                     ATDTLGHVAEGVYSARTVLQRAARLGVDMPIAQAVVAVLDGQMTPPQALAQLMGRDPV
                     AELATLA"
     gene            4044..4922
                     /gene="nadC"
                     /locus_tag="PLB25_12915"
     CDS             4044..4922
                     /gene="nadC"
                     /locus_tag="PLB25_12915"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HOE42515.1"
                     /translation="MTATFDFSPASVAALAQADVARALLEDVGAGDLTAGLVDPTRRA
                     RAIVVARETAVICGAPWVQQAFMQIDPGAEITWRIAEGQRCEADQTVLEVVGQARALL
                     TAERTALNFLQLLSAVATKTNNYVQAIREVPQVRASIVDTRKTIPGLRLAQKYAVRTG
                     GGVNHRLGLHDAVLIKENHIAAAGGVTAVLKRAAETAPSAKFVQIEVEDLDQLREALQ
                     AGATMILLDNMGLSQLHEAVHITDGRALLEVSGGVNLSSVCAIASTGVDRISIGALTK
                     SVNATDFSMRFVDAPV"
     gene            4964..>5433
                     /gene="nadA"
                     /locus_tag="PLB25_12920"
     CDS             4964..>5433
                     /gene="nadA"
                     /locus_tag="PLB25_12920"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HOE42516.1"
                     /translation="MSEQVLAMKEVDYEQPLAKAPAGDSVCATKHAWARVPVEPNAAE
                     RAALKDRIRGLLKEQNAVLVSHYYVHPDLQDLAEETGGLVSDSLEMARFGRDHAATTL
                     VVSGVRFMGETAKILSPEKRVLMPDPDANCSLDLGCPLDAFSAFCDAHPDRTVVV"
BASE COUNT         1075 a         1582 c         1603 g         1173 t
ORIGIN      
        1 tcttcacgtg ccttttcacg caccggatcc ttgatttcct caccctggcc gctgctcagg
       61 tgtttgtcaa agtcagcctc gcgcagcttg agtgcggcaa acagattgcc ttcctcggtt
      121 tcatcaatca tcacatcggg cacgatgcct ttggcctgga ttgacttgcc gctgggcgtg
      181 tagtagcggc tggtggtcag cttcagcgcc gcagtggggc agttgtccat gcgaaacgcc
      241 gagtccaggc aaacagcggt ttgcaccgaa cccttgccaa aagtctggct acccaataac
      301 ttggcgcgtc tgtggtcctg cagggcaccg gctacgattt cactggccga ggcagagcct
      361 tcattgacca gcaccaccag cgcgaccgac ttaagcgccc cgtgggtgac ttcttccagt
      421 tgctttaacg gatctggccc gcgccgtgca taaaaccgcg gcgatgcctt ataggtgaat
      481 ttgctttccg ccaattgacc actttgtgac accacggtca cgttttccgg caaaaacgcc
      541 gcagaaattg ccaccgcagc atccagataa ccacccgggt cgttgcgcaa atcgagcacc
      601 agtcccttga gcttgggttc ttgcttgtag atctcttcca tcttgcgcgc gaagtcggct
      661 accgtgcgct cctgaaactg gcttagccga atccaggcat agcccggctc aatgaccttg
      721 ctgcgaaccg actgggtgcg aatttcttct cgcacgatgg tgaccggaaa ggtgcggctt
      781 tcatccttgc ggtaaatgct cagcaacacc ttggtattgg gctcgccgcg catgcgcttg
      841 acagcttcgt tgagcgtgag ccccttgact ggcgtgtcgt caattttgac aatcaggtca
      901 ttgggcttga gtccagcccg aaaggcgggt gagccttcaa tcggggacac caccttgacc
      961 aaaccgtctt cttgcgaaat ttcaatgccg accccgacaa atttgccggt ggtaccttcg
     1021 ttgaaatcct tcagtgcctt cttgtccaga taaaccgagt gcggatcaag ccccgccacc
     1081 ataccggcaa tggcatcggt gatgagcttt ttctcgtcca ctggctcgac atagttgctt
     1141 ttgaccatgc caaacacggc cgcgagctgc tgcaactctt ccagcggcaa tggggccagc
     1201 gtgccgcgcg ccacggtttg caacgacacg gtggtcaaga ccccagccat gacaccgagg
     1261 gaaatccagc cagtaatctt caatttgtga cccatagtaa ttccagaacg tgtaaaagcg
     1321 gttcaattcg agccattttg agtctagcca gcgacaccgg ttcccgaatc aaaaaagaaa
     1381 tgtttctggt tttgcttcgc gcttgcccca ccggcactgt catgatcaac acccaggcat
     1441 caccgggacg gcaatgatag atggttttgg tggcaacgtc gcgctggcaa acctttcaaa
     1501 cacggttggt tgtagccaac cccacgctat ggcgcgcacg ccagaatatt gttcaaagcg
     1561 ggtcaggcgg ctaagctgct ttttttgagt cttgatcgac tcaggtggca aatagctgca
     1621 agaccattca cgctatttcc ggcggggctg ctagcgcaag cgccgcattc taaaatagcg
     1681 gcttagtttc ttcgtctgac cttaaggatc cccaagtgaa attttttctc gataactgga
     1741 tgttgatcgc cgtggcgctg tcagcgggag gcatgttgct atggccaatg atcagcagcg
     1801 gcgggccggg ggccctgacg gccgatggtg ccgtgcagtt gatcaaccgg gaaaaggcgg
     1861 tggtgattga tgtctgcgag cccactgaat atgccagcgg ccatgtttcc ggggccaaga
     1921 atatcccgct gggtcagctc gaaggcaagc tggcatcaac cgtcaaaaac aaggctttgc
     1981 cggttatcct ggtgtgcctg tctggtgcgc gcagcaaacg ggcggtatcg attgccaaga
     2041 agcttgggtt tgagaatgcc gtctcgctgt caggcggcat gaacgcctgg cgctcggcca
     2101 atttgccggt tgaaaaagcc tgaaccttgc ggagcgattc atgtcaccag tgaagatgta
     2161 caccacagcg gtctgcccct attgcattcg cgccaaacag cttttgaaag cccgcggagt
     2221 cgagcacatc gatgaaatcc gcattgacct ccatcccgat gaagtgcagg agatgatgcg
     2281 cctgaccggt cgtcgcaccg tgccgcaaat cttcatcggg aacacccatg tgggtggttg
     2341 cgacgatctg atcgcgctcg atggccaggg tggtctgctg ccactgctgt caaacgcctg
     2401 agataatctg gcccttgcct atgttgcccg gcgcagcggt tgcgtcgggt tttctttttt
     2461 tgttaccgaa agaaacccat gtccgagtcc caagatcccg ttttccagat tcagcgcgtt
     2521 tacctcaaag aagcctctct tgagcaaccc aactccccgg ccatcctgct cgaacaggaa
     2581 cagcccacgg ttgacatcca gttgggcgtg aatgcctcgc cggtggccga gggcatttat
     2641 gaagtggcgg tcacggccac cgtgcacacc aagatcaagg acaagaccgt gtttttggtg
     2701 gaaatcaccc aagccggtat ttttgaagtg cgcaacctgc cgcaagagca gatgggtccg
     2761 gtcatgggca ttgcctgccc gcaaatcgtc tatccctacc tgcgtggcaa cgttgctgac
     2821 ctgattcagc gcgctggctt cccgccggtg catctgtcgg aaatcaattt ccaggccatg
     2881 tacgagcagc agcagcaaca gcaggccgct ccagccgaag gcgcgccgcg catcgttacc
     2941 cagtagtttg aaggtgtttt gagactgcag ccctttctgc tattgcacaa gcagctatga
     3001 aaatcgtagt attaggggcg ggtgcctggg gcaccgcgct ggcggtcagc gcggtcggac
     3061 aacaactggc gcagcaccag gtcacgctct gggcgcgcag ccccctacaa accgaacaca
     3121 tgctggcgca gcgcgaaaac cagcgttatc tggccggtgt gccattgccg gccagtttga
     3181 ctttgagcgc tgcacccttg ccgcagctcg ccacgctggc gcagcatgcg gatctggtgg
     3241 tggtggccac gccaatggca gcattgcgcc agaccctgtc gttgttgcgt gactgcaggg
     3301 cccccgtggc ctggctttgc aaaggttttg aattgccagt ttctgaaggt tttgggcttc
     3361 tagcccatga gatacaagcg caggtagctc cttgtttaat agcaggcgta ttcagtggcc
     3421 cgagctttgc gcaagaagtc gcgcgcggtc aaccgaccgc actggtagct gccagcccgc
     3481 acgagtcggt gcgccaggcc ttggtcgagg cgtttcacgg ccgcagcctg cgggtttacg
     3541 ccaacgatga catggttggt gtggaagtcg gtggcgcggt caaaaacgtg ctggccatcg
     3601 gcactggctt gtgcgacggc ttggcgctgg ggcaaaacgc ccgcgcagcc ctgatcacca
     3661 ggggcttggc cgaaatgctg cgcctgggga ttgcgctggg tgcccgggct gaaaccttca
     3721 tgggcttgag cgggctgggt gacctggtgc tcaccgccac gggtgatttg tcgcgcaatc
     3781 gtcaggtcgg tctggcgtta gcccatggac gcacgctagc gcaagccact gatacgctgg
     3841 gtcacgtggc agagggcgtt tacagcgcgc gcacggtttt gcagcgcgcc gcccggctgg
     3901 gcgtggacat gccaatcgcc caggcggtgg tggccgtgct ggatggccag atgacaccgc
     3961 cacaagcgct ggcgcagctt atggggcgtg accccgttgc cgaattggca acgctagcct
     4021 aaactcaata acaggagaac aacatgacgg ctacttttga tttttccccc gcctctgtgg
     4081 cggccctggc ccaggccgat gtggcgcgtg ccctgctcga agatgtcggt gctggcgacc
     4141 tgactgccgg tctggttgac ccaacccgtc gcgcccgagc aatcgtggtg gcgcgcgaaa
     4201 cggccgtcat ttgcggagcc ccttgggttc agcaggcatt tatgcaaatc gacccggggg
     4261 ctgaaatcac ctggcgcatc gctgaagggc agcgctgcga ggccgatcaa accgtgctgg
     4321 aggtggtcgg ccaggcccgt gcactgctga ctgcggaacg cacggcgctg aattttctgc
     4381 aattgctcag cgctgtggca accaaaacca acaactacgt gcaggccatt cgtgaggtac
     4441 cacaggtgcg cgccagtatt gttgacaccc gcaaaaccat ccctgggctg cggctggcgc
     4501 aaaagtatgc tgtgcgcact ggcggcggtg tcaatcaccg gctgggtttg catgacgcgg
     4561 tactgatcaa ggaaaaccac atcgccgccg ccggtggcgt gacagccgtg ctgaaacgcg
     4621 cggcagaaac agccccctcg gccaagtttg tccagatcga agtggaagac ctcgatcaac
     4681 tgcgtgaggc gctccaggcc ggtgccacca tgatcctgct cgacaacatg ggtttgtcgc
     4741 agttgcacga agccgtgcac atcaccgacg gtcgggcctt gctggaggtc tccggcggtg
     4801 tcaatctgtc aagcgtgtgt gccattgcgt ccaccggggt tgaccgcata tcgattggtg
     4861 ccctgaccaa aagcgtcaat gcgacagatt tttccatgcg ttttgttgac gcaccagtct
     4921 gaatcagacc gggttgccat tcgttttgat tgcaggagtt gtcatgtctg agcaggtttt
     4981 ggcaatgaaa gaagttgact atgagcagcc gctggccaaa gccccggccg gcgactcggt
     5041 gtgtgccacc aaacatgcct gggcgcgggt gccggttgag cccaatgcag cagaacgtgc
     5101 cgcgttgaaa gaccggattc gcggtctgct taaggaacaa aacgcggtgc tggtgtcgca
     5161 ctactatgtg cacccggatt tgcaggacct ggccgaagaa accggtggcc tggtcagtga
     5221 ttcgctggag atggcgcgct ttggccgcga ccatgccgcc accacgctgg tggtgtcggg
     5281 cgtgcgtttc atgggtgaaa ccgccaaaat cctgtcaccc gaaaagcgcg tgctgatgcc
     5341 cgaccccgat gccaattgct cgctcgacct gggctgccca cttgatgctt tcagcgcttt
     5401 ttgtgatgcc cacccggacc gcacggtggt ggt
//
LOCUS       DAOSLL010000294         5424 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13512_length_5424_cov_4.322406, whole genome shotgun sequence.
ACCESSION   DAOSLL010000294 DAOSLL010000000
VERSION     DAOSLL010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13512_length_5424_cov_4.322406"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3112
                     /locus_tag="PLB25_12925"
     CDS             <1..3112
                     /locus_tag="PLB25_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42517.1"
                     /translation="QILGGGLIASDTYGLGAAGNVAVKAGSLLLDGSGHANAARITSR
                     SGVGAVGNAGSVAVDVADSIVLSNVGKITSDSFGTGQGGNISLHAGKDIQVLTDSYIA
                     SDAYATGNAGAIDISAQNLTIDGTGGKLLTQVSSQAYAGSQGNAGSIHVTTSDALNLF
                     NGGKIISDTDSVGTAGDISVTTGTLLLDGRESTLSTGIFSAASFDSSGNGGTINVRVA
                     GDAQILQSARVGSATYGKGRGGDVLFTANNLLIDALGSPYGAGISSGANETSSGDGGS
                     VTVDVVGHLQLIDAGQISATTWAAGNAGSVSVTAGKLTIDGKDRVGTAGIDSSSLSIA
                     GNTKLGNTGQVTVTVAGDALLTHGGVIQSSSQANFPTASDTTSQTVAGSSSPVGDIKL
                     VIGGDLKVEEKAGISTSTVGTSNSGNIEIQVGGNMTVGVDVLIASATFGTGNGGNIGI
                     QVGGNMTVVEGLVASSTHGTGNGGSVGIQVAGNMTVDDGGIVTSGAYGTGNGGTVGIR
                     VAGNMNIGADGLIDSSTTGVGNSGNIGIQVSGKLDVGSGAWISTKTSGSGAAGNLDIS
                     ASQLAMAGGGVGHDAWIMSDSLGSGAAGRIDVKASKSIELAENSWITSDSRGSGHAGT
                     VSVSAPDIRIGGTELVQGAGISSSAFSSGDAGRVEVNAQTLTVMGGKAVYATGIESRS
                     GKEASGNAGTVVINVSGALQVLEGGAIASSTELSGRGGEVLVKAGSIEVNGEGSQIGA
                     AAKATSSGQPGNVSVHADGALVVASGGSLSIQNDGTSAQPSSVSPSLLSVEAAQLILI
                     DAGAIKANATGNVAAGTLQINVSDRLSLHDSSITTSANSGNGGPISIQAGQVVDLNRS
                     QITTSVLGSVGSGGDIHIAAGALALNSGFIQANTAASNASGGLVDIEVKNLVASGNTL
                     WVGGQSPLDFAPDVFGFNVIQAAAPTGVSGAIDITSPVLDVSGSLRGLDAQVMDAGGL
                     GRNPCQTSGGSSLVQIGRGGLPPSGRGLLRGEPFAAPALDPMDPLAWSGSEKTNPVRL
                     PTGSCQ"
     gene            3109..4806
                     /locus_tag="PLB25_12930"
     CDS             3109..4806
                     /locus_tag="PLB25_12930"
                     /inference="COORDINATES: protein motif:HMM:NF015803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HOE42518.1"
                     /translation="MMSLACEKSLFAGAVLCLSLLATLVQAADPAHLPATSTTRPPDY
                     LKPDAGAFQLPTLDNQLTIPTLTGSTERVRVGRVDFEGNTVLATPVLQAIAAPYVGRV
                     VTVAELEALRRALTLAYVERGYVNSGVLLPRQIVDGVLVFSVVEGHLSAIRLHGMQRL
                     DQRYLTQRLAPDKDVPLNIEVLRERFQLLLADPLISRLNARISPGVAPGEAMLDIDVT
                     RARSYQFSAFANNYRPASIGSESVGVTAMVHNLTTRGDILEASVQSPPHFGQGTHSTL
                     AWQMPLGFAGTRINIALERGSSSVVEEPTNVLDIRSTLDSTDVGLSRVVFESLRQKLA
                     LGLNRVSRKNRTWLLGEPFSFTPGDPTGSTQETLWRFWQDYSVRSETQVLALRSTLTY
                     GKNSLQDTAGLPESEVLARQFHLWLGQAQFGRQVMDNGAQLVLRATLQHTRDRLLALD
                     GLAIGGVNTVRGYRENQLVRDKGAFVNFEFEYPLLREAADGLSLTLIPFYDYGRAQNV
                     GEAAVTLSSWGLGSRLQWQRFNLDLVLAKRLSHPDSVSGNGSTLQDHGVHLQLAYRF"
     gene            complement(4838..>5424)
                     /locus_tag="PLB25_12935"
     CDS             complement(4838..>5424)
                     /locus_tag="PLB25_12935"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE42519.1"
                     /translation="LSWLMINGRERALRLAAAMTEELRHMAQHDPLTDLPNRALFSDR
                     LNHELARAKRQHGRFAMVFLDLDHFKPINDKFGHGVGDQVLRWVARQLQNSVRAADTV
                     GRIGGDEFVVLMAQLSRSDSILALAEKLRQALDQPFVVEGHELSISCCIGVAVYPEDG
                     TDAITLTKGADDAMYRAKEEGGDCVRLCQPVNPS"
BASE COUNT         1106 a         1524 c         1688 g         1106 t
ORIGIN      
        1 ccagattctg ggcggcggat tgatcgcatc tgacacctac ggcttgggtg ctgccggcaa
       61 tgtagccgtc aaggccggat cgttgttgct ggatggatcc ggacatgcaa acgctgcccg
      121 catcaccagc cgcagcgggg tcggcgcagt tggcaatgca ggatctgttg ccgtcgatgt
      181 tgctgattcc atcgtgctat ccaacgtcgg caagatcacc agcgacagct ttggtaccgg
      241 tcaaggcggc aacatcagct tgcatgccgg aaaagacatt caggtcctca cagatagcta
      301 tatcgcctcg gatgcttatg ctacgggcaa cgccggagcc atcgatatat cagcacaaaa
      361 cctgacgatt gacgggaccg gcggcaaact tctcactcag gtatcgagtc aggcctatgc
      421 aggcagtcag ggcaatgctg gctccatcca cgtcaccact tccgatgcct tgaacctgtt
      481 caacggcggc aaaatcatca gcgacaccga tagtgttgga acggctggtg atatcagcgt
      541 gacaactggc acactattgc tggatggccg tgaatcgaca ctttcgacgg gcattttcag
      601 cgccgccagt tttgacagca gcggaaatgg tggcacgatc aatgtccgcg tcgctggcga
      661 tgcgcaaata ttgcaatccg ccagggtagg ctctgctact tatggcaagg gtcgtggcgg
      721 tgacgtttta ttcaccgcca acaacctgct gattgatgct ttaggcagcc cttacggagc
      781 gggcatcagt tcaggtgcca atgagaccag cagcggcgac ggaggatctg tcacagttga
      841 cgtggtcgga catctgcaac tcatcgatgc cggccaaatc tcggctacca cctgggcagc
      901 cggcaatgca ggatcggtgt cagtaacggc tggcaagctg acaatcgacg gcaaagatag
      961 agtcggtact gcaggcattg atagctccag cctgtcaatt gcgggcaaca caaagctcgg
     1021 caataccgga caggttacgg taacagttgc cggagatgcc ctgctgacgc atggaggtgt
     1081 cattcaatcg agctcacaag ccaacttccc aacagcgtca gacaccacca gtcagacagt
     1141 tgccggatca agcagcccgg ttggcgacat caagcttgtt attggcggtg acttgaaagt
     1201 cgaagaaaag gcgggaatca gcacttcaac ggtcggaacc agcaatagtg gcaatatcga
     1261 aatccaggtg ggtggcaaca tgacggtcgg tgttgatgtg ctgatcgctt ccgcgacgtt
     1321 tggaaccggc aatggcggca atatcggaat ccaggtcggt ggaaacatga ctgtcgttga
     1381 agggctggtc gcttcctcga cgcatggaac cggaaatggc ggcagcgtcg gcattcaggt
     1441 ggctggcaac atgactgtcg acgatggcgg gatagtcaca tcgggagcat atggaaccgg
     1501 caatggtggc acggtcggaa ttcgggtagc tggaaacatg aatatcggtg ccgatgggtt
     1561 gatcgattcc tcgacaaccg gggtcggcaa tagcggcaat attggaattc aggtgtctgg
     1621 aaagctggat gttggcagtg gtgcgtggat atcaacaaaa acctcgggca gtggtgcagc
     1681 gggcaacctt gacatttcag cgagtcagtt ggcaatggca ggtggcggag tcggacatga
     1741 tgcctggata atgagtgact cgctcggttc cggtgccgcc gggcgtatcg atgtgaaagc
     1801 gagcaaaagc attgagttgg ccgagaacag ttggattacc tcggattccc gcggcagcgg
     1861 ccatgcagga acggtttcgg tcagtgcccc tgacatccgg attggcggca ccgagttggt
     1921 tcagggtgct ggtatctcca gcagtgcttt ttcatcaggt gatgccggcc gtgtcgaagt
     1981 gaatgcccag acgttgacgg taatgggtgg aaaagcggtc tatgcgactg gcattgaaag
     2041 tcgaagcggg aaagaggcct cgggcaatgc cggcacggtg gtgatcaatg tcagtggtgc
     2101 ccttcaagtg cttgaaggtg gtgcgattgc gtcaagcaca gagttgtcag gccgcggcgg
     2161 cgaggtgctg gtcaaggctg gttcaatcga ggtgaacggc gaaggcagcc agatcggtgc
     2221 ggcggccaag gcaacatcaa gcggccagcc aggcaatgtt tcggttcatg ccgatggcgc
     2281 actggtagtt gcatccggcg gcagcttgag catccaaaat gatggcacaa gcgcccagcc
     2341 gtcatcagtg tcaccgtccc tgctgagcgt ggaagcggcg caactgatcc tgatcgacgc
     2401 tggtgccatc aaggccaacg ccacgggcaa tgtggctgcg ggcacactgc agatcaacgt
     2461 cagtgatcgc ttgtccttgc atgacagcag catcaccacc agcgccaaca gcggcaatgg
     2521 cggcccgatc agcattcagg caggccaggt ggttgacctg aaccgctcgc agatcaccac
     2581 atcggtgcta ggctcggtcg gcagtggtgg cgacattcac atcgctgccg gggcgctggc
     2641 tctgaattct ggtttcattc aggccaacac ggctgccagc aatgcgtcag gtgggctggt
     2701 ggacattgag gtcaaaaacc tggtcgccag cggcaatacc ttatgggtgg gtggtcagtc
     2761 gccgcttgat tttgcgcccg atgtttttgg ctttaacgtg atccaggccg ctgcacccac
     2821 cggggtcagc ggtgccatcg acatcaccag cccggtgctt gatgtctctg gcagcctgcg
     2881 cggccttgac gcgcaggtga tggatgccgg tggcctgggc cgcaacccct gccagaccag
     2941 tggcggcagt tcattggtgc agatcggtcg cggtggcttg ccaccttccg gtcgggggct
     3001 gctgcgtggc gagccgtttg ccgcaccagc acttgacccg atggatcctt tggcttggtc
     3061 tggcagcgaa aagaccaacc cggtgaggct gccaaccggg agttgccaat gatgtcattg
     3121 gcctgtgaaa aaagcctgtt tgctggcgct gtgctgtgct tgagcctgct ggcgacgctt
     3181 gtacaagcgg cggacccggc gcatctgccc gccacttcga ccaccaggcc acccgactac
     3241 ctgaagcccg atgcaggagc gtttcaactg ccaacgcttg ataaccaact gaccatcccg
     3301 acactgaccg gcagcaccga gcgggttcgc gtcgggcggg tcgattttga gggcaatacg
     3361 gtgctggcaa cccctgtgtt gcaggccatt gccgcacctt atgtcgggcg ggtcgtcacg
     3421 gtcgccgaac ttgaagccct gcgtcgcgcg ctaaccctgg cctatgtgga gcgcggctat
     3481 gtcaattccg gtgttctgct gcccaggcag atcgtcgatg gcgtgctggt cttttccgtg
     3541 gtggaaggcc acctgagcgc cattcggctg cacggcatgc aacgtctgga ccagcgctac
     3601 ctgacccaac gtctggcacc agacaaggat gttccgctca atattgaggt gctgcgcgag
     3661 cgctttcagt tgttgctggc cgatccgctg atttcgcgct tgaatgcgcg catatcccct
     3721 ggcgtggcac ccggtgaagc catgctggat atcgatgtga cacgggcgcg ctcttaccag
     3781 ttcagcgctt ttgccaacaa ctaccgaccg gcctcgatcg gctccgaatc tgtcggcgtg
     3841 actgccatgg tgcataacct gacaacgcga ggtgacattc tggaggccag cgtgcaaagc
     3901 ccgccgcact tcggacaggg tacgcacagc acactggcct ggcagatgcc gctagggttt
     3961 gccggaaccc ggatcaacat cgctttggag cggggcagtt cttcggttgt tgaggaaccc
     4021 accaatgtgc tggacattcg cagcaccctc gacagcaccg atgtcggctt gagccgggtg
     4081 gtgtttgagt cgctgcgcca gaaattggcg cttggactga accgtgtcag ccgcaagaac
     4141 cgcacctggc tgctgggtga gcctttttca ttcacgcccg gcgaccccac cggtagcacg
     4201 caagagaccc tgtggcggtt ttggcaggac tattcagtac gctctgagac ccaggtgctg
     4261 gcgctacgct cgacgctgac ctatggcaag aattcgctgc aagacacggc cggactgcct
     4321 gagagcgagg tactggcaag gcaattccat ttgtggctgg ggcaagccca gtttggccga
     4381 caggtcatgg acaacggcgc gcagttggtg ctgcgggcca cgctgcagca cacccgggac
     4441 cgcctgctgg cgctcgatgg cctggctatt ggcggtgtca acacggtgcg cggctaccgg
     4501 gaaaaccagt tggtgcgtga caagggtgcc ttcgtcaatt ttgaatttga gtatccactg
     4561 ttgcgtgaag cggcagacgg cctgagcctg acgctgatcc cgttttacga ctatgggcgg
     4621 gcgcaaaatg tgggtgaagc agcggtcacc ctgtcgtcct ggggcctggg cagccgactg
     4681 cagtggcagc ggtttaatct ggacctggtg ttggccaagc gtctgagcca ccccgatagc
     4741 gtcagtggca atgggtcaac gttgcaagac cacggcgttc atttacaact ggcgtacagg
     4801 ttttgagcta cggcttgaaa gaaccattct gactgcatca gctcgggttt accggctggc
     4861 acagccgaac acaatcgccg ccctcctcct tggcacggta catggcatcg tccgcaccct
     4921 tggtcagggt gatggcatcg gtgccatctt ccggatagac ggcgaccccg atacagcatg
     4981 aaatcgacag ttcatgccct tccaccacga atggctggtc cagcgcctgc cggagctttt
     5041 ctgccagggc gagaatggag tccgacctgc tgagttgcgc catcagcacg acgaattcat
     5101 caccaccaat gcgcccgact gtgtctgcgg cccgaacact gttctgaagt tgcctggcga
     5161 cccatcgcaa tacctggtcg cccacgccat ggccgaactt gtcgttgatc ggcttgaaat
     5221 ggtcgaggtc cagaaacacc atggcaaagc gcccgtgctg gcgtttggcg cgcgccagtt
     5281 catggttcaa ccggtcgctg aacagggccc gattgggcag atcggtcagc gggtcgtgct
     5341 gcgccatgtg ccgcaattct tccgtcatcg ccgcagccag acgcagcgcc cgctcccgcc
     5401 cgttgatcat cagccagctc agca
//
LOCUS       DAOSLL010000295         5409 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13562_length_5409_cov_3.286702, whole genome shotgun sequence.
ACCESSION   DAOSLL010000295 DAOSLL010000000
VERSION     DAOSLL010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5409)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13562_length_5409_cov_3.286702"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="PLB25_12940"
     CDS             complement(<1..194)
                     /locus_tag="PLB25_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I CRISPR-associated protein Cas7"
                     /protein_id="HOE42520.1"
                     /translation="MNTPIKNRYEFLYLFDCENGNPNGDPDAGNSPRIDPEDMHGLVS
                     DVAIKRRVRNYVQMKHANGG"
     gene            complement(282..1952)
                     /gene="cas8c"
                     /locus_tag="PLB25_12945"
     CDS             complement(282..1952)
                     /gene="cas8c"
                     /locus_tag="PLB25_12945"
                     /inference="COORDINATES: protein motif:HMM:NF021243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-C CRISPR-associated protein Cas8c/Csd1"
                     /protein_id="HOE42521.1"
                     /translation="MLHALREYGAKLGGEPGFKSREVRWCIQLSEVGQLLNIVPLGDG
                     KAGVMLEACPDMHAMNSGGKAHFLVESAQTIARHFKDGEQPAKVDSANARHQYYSKML
                     AQAAGDLAVLSPPSKLLCDPVALDQIRAMMVAKRIKPADWVTWQVGAFDPREDTGVQQ
                     WWRDWRLQDLGGGVETGDSLPAGNMLCLLTGDPVEPLLTQPKITGLSGVGGLSMGDVM
                     VGFDKAAFQSYGLEKSTNAAMGEAPAQQYVDALNHLIRQQKDATSKNPSRRTNAMMVY
                     WFKEHIPPEDDPFSMLYGMETGAQQTASALSQARKLLDAIRSGSRSALGNNHYYAMTL
                     SGASGRVMVRDWMDGQFVQLVSNIEAWFSDLAIVHREGGNRLAPDSKFLAVGGSLVRD
                     LKDLPASTTSVLWHAAVAQLPIPQPLMAQALDRFRSAIVKDETFSHARMGLIKAYFVR
                     QPQGKALMQPYLNPVHPDPAYHCGRLLAILAKLQHAALGDVGAGVVQRFYAIASTAPG
                     LTFGRLVGNSRNHLGKLEGGLSYWFEQQIAEVMGQLGDQFPRTLNLEG"
     gene            complement(1955..2740)
                     /gene="cas5"
                     /locus_tag="PLB25_12950"
     CDS             complement(1955..2740)
                     /gene="cas5"
                     /locus_tag="PLB25_12950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas5"
                     /protein_id="HOE42522.1"
                     /translation="MPNTSQPNPPNTHTLEVWGDLACFTRPEMKVERFSYPVITPSAA
                     RGVFDAIYWDGKREQEKVGKKTRDIIRPYFFWQITRIEVLTMPSLIALRRNEVKGVVP
                     GAVTLNKWMTGKEAPEPLWADGDDETTGRTQRQTMALKNVRYRITARIEPRASHRNQL
                     AKLNACFERRAKGGKCFQQPYLGCREFPAFFEYIEPGAPSAARAPMDQQLGWMLYDVF
                     DLSNEAPGLDGKPFISVFDACVKSGVLDVPAYGSDAVRKPGRV"
     gene            complement(2772..5111)
                     /locus_tag="PLB25_12955"
     CDS             complement(2772..5111)
                     /locus_tag="PLB25_12955"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
                     /protein_id="HOE42523.1"
                     /translation="MTSYFAHSENDHGKLHLLKQHLGCVSDLGGNFAAGTTWQSEAVL
                     AGLLHDLGKYGDLFQARLRGQEAGLDHWSAGAVAALKPYGALAAALAIEGHHIGLQAA
                     ASDALSQRLGMVLKGASPVGPNIRLTDANLNRLLERAQVDGLVFEQPLKPMIPLGQPD
                     AWQQAIARMLDVRMLFSSLVDADYLDTEAHFNGTSEGKNYRQTGLPLDAARAVLALDM
                     FMNLSVRRGTSQDRSSDAVAQTRAELWNAVTQAAIKPTGIFSLTAPTGSGKTLAMLQF
                     ALAHAKQNGLKRVVLAVPYLSIIEQTARIYHAVFADDPEIFILEHHSLAGVGVEQSDP
                     HKCNDAERQRRLYAENWDAPIILTTNVQLLESLFSNRSSACRKLHRLRDSVILFDEAQ
                     SLPQHLAVPTLAALSHLALDYNNSVVFATATQPAFDTLDIAVRKHAASGWQPTEIVPG
                     HAAMFVRLKRVEVQWPAADESLSWQALADSLCADDAAQVLCVLNLKRHAHARLKAMAG
                     TSGLRHLSTNLCTAHRRKVLEQVRKCLDPANPQPCRLVSTQCVEAGVDLDFPLVYRAM
                     APLEAVAQAAGRCNREGRLNGWGRFGQVRVFEPALDTSERKRMFPTFAYFQATEVTAS
                     LLRENGGNLSINDPAVFRGYYQRLYGVSRPVDQNVDLTNAIAALDFPEIARCYQLINQ
                     DAIQIVVPWKPCIAEFKRLRDLASSGIDGQWMKAAQALTVCIYRPKPDHPAWGCLIAA
                     QFRRGGSSDEWFILEDPDERFYDDIVGLQLPQNQMVMIA"
     gene            complement(5153..5347)
                     /locus_tag="PLB25_12960"
     CDS             complement(5153..5347)
                     /locus_tag="PLB25_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42524.1"
                     /translation="MKFIPPPNMASPEPQRSNKPIAHQREEDGITQSLEAHLLGVSHL
                     AGAFAAKLGLNEQGDREVVA"
BASE COUNT         1277 a         1515 c         1406 g         1211 t
ORIGIN      
        1 ctgccaccat tagcgtgttt catctgcaca tagttgcgca cacggcgctt gatggcgaca
       61 tccgacacta gaccatgcat gtcttcggga tcgatacggg gactgttacc agcatccggg
      121 tcgccatttg gattaccatt ttcacaatcg aagaggtaaa gaaattcgta gcggttcttg
      181 ataggtgtat tcatgtggat tctctttccg gtattgtgat tactgctttt ctatttgcgc
      241 aaggccgcaa gttgctggta ataacccaac gcaaaaaggc cttacccctc caggttcaga
      301 gtgcggggaa attggtcacc caactgcccc attacctcgg caatttgctg ctcaaaccaa
      361 taggacaagc caccttcaag tttgcccaaa tggttgcgcg aattgccgac caaacggcca
      421 aaagtaagcc ccggtgccgt gctggcgatg gcataaaagc gctgcacaac accggccccc
      481 acatcgccga gcgcagcgtg ctgcagcttg gcaagaatcg ccaggaggcg cccgcagtga
      541 taagccgggt caggatgaac cgggttgaga tagggttgca taagcgcctt tccttggggt
      601 tgacgaacaa aataagcttt gatcaggccc atgcgggcat gactaaaagt ttcatctttg
      661 acaatggcag agcgaaagcg gtctagcgcc tgcgccatca aaggttgggg aataggcagt
      721 tgtgcaaccg ccgcatgcca taacaccgaa gtagtggacg ctggcagatc tttaagatca
      781 cgaactaacg atccgcctac agccagaaat tttgaatcag gagctaaacg gttccccccc
      841 tcacggtgca cgatggccag atccgaaaac catgcctcaa tattcgaaac aagttgaaca
      901 aattgcccat ccatccagtc ccgtaccatc acccgcccgg aggcgccaga gagagtcatg
      961 gcgtagtagt ggttattgcc caacgcggaa cggctcccgc tgcgaatagc atcaagtaac
     1021 ttacgcgctt gagacagggc agatgcagtc tgctgagcgc cagtttccat gccatagagc
     1081 atggaaaagg ggtcatcctc cggggggatg tgttccttga accaatacac catcatggca
     1141 ttggtgcgcc gggacgggtt cttgctggtg gcgtctttct gctgccgaat gagatggttc
     1201 agcgcatcca cgtattgctg tgcaggtgct tcccccatgg ctgcgttggt tgatttttcc
     1261 agaccgtagg attggaacgc agccttgtca aagccgacca tgacatcacc catggacaaa
     1321 ccaccaacac ctgacaggcc tgtaattttt ggctgtgtca gaagcggttc aacagggtca
     1381 ccagtcaaca aacacagcat gtttcccgcc ggaagactgt caccagtttc gacaccacca
     1441 ccaaggtctt ggagtcgcca atcacgccac cattgctgca cgcccgtatc ttcacgcggg
     1501 tcaaatgcac ccacctgcca agttacccag tctgccggtt tgatgcgctt tgcaaccatc
     1561 atggcgcgaa tctgatccaa agcaactggg tcacacaaca gttttgaagg gggcgacaag
     1621 actgccagat cacctgccgc ttgcgctagc atttttgagt agtactgatg cctggcattt
     1681 gcagaatcga ccttggcagg ttgttcgccg tccttgaagt gccgtgcaat ggtctgcgcg
     1741 gattcgacta gaaagtgcgc tttgccacct gaattcatgg cgtgcatatc agggcaagct
     1801 tccaacatga ctccagcctt gccgtcgcca agtggcacga tgttcagcaa ttgccccacc
     1861 tcagagagct gaatgcacca gcgtacttct ctggacttaa aacctggttc accacccagc
     1921 tttgcaccgt actcccgtaa agcgtgcagc atggctaaac ccttcctggc ttgcgcaccg
     1981 cgtcgctgcc atacgctggt acatccaaca cgccactttt cacgcacgca tcaaaaaccg
     2041 aaataaatgg tttgccgtcc agcccaggag cctcgttgga caggtcaaac acgtcataca
     2101 gcatccagcc cagttgttga tccatcggtg ctcttgctgc acttggtgcg ccgggttcga
     2161 tgtattcaaa aaatgccggg aattcgcggc atcccagata gggctgctga aaacacttgc
     2221 cgcccttggc gcgacgttca aaacaagcgt tgagcttagc caactggttt cggtgactgg
     2281 cacgcggttc aatgcgggcg gtaattcggt aacgcacgtt tttgagggcc atagtctggc
     2341 gctgggtgcg cccggtagtt tcatcgtcac catctgccca caatggctcg ggcgcttctt
     2401 tgccggtcat ccatttattg agtgtcaccg cgcccggcac aacacccttg acttcattgc
     2461 gccgcagggc gattaagctg ggcatggtca gcacttcaat gcgcgtgatt tgccagaaaa
     2521 aataggggcg aatgatgtcg cgcgtcttct tccctacctt ttcctgctca cgcttgccat
     2581 cccaataaat ggcatcaaag acaccgcgtg ccgcagaggg ggtgatcacg gggtaagaaa
     2641 agcgctccac cttcatctcg ggtctggtga agcaggccaa gtcaccccag acttctagcg
     2701 tgtgggtgtt gggtggattg ggttggctag tgtttggcat gctgggaaac tcctccttga
     2761 aaatgtggaa atcaggcgat catgaccatt tggttttgcg gcaactgtag gccaacgatg
     2821 tcatcgtaga accgttcatc cggatcttcc agaatgaacc actcatccga actaccacca
     2881 cggcgaaatt gcgccgcaat caagcagccc caggcggggt gatctggttt gggtcgatag
     2941 atacagaccg tgagggcctg tgctgctttc atccattgac catctatacc gctggaggcg
     3001 aggtcgcgca gccgcttgaa ttcggcaata catggtttcc aaggcacaac gatctgaatg
     3061 gcatcctggt tgatcagttg gtagcaacgc gcaatttcag gaaaatcaag ggctgcaatg
     3121 gcattggtca agtcaacgtt ctgatcaacc gggcggctta caccgtagag tctttggtaa
     3181 tagccgcgaa acacggctgg atcattgatg ctcagattgc caccgttctc gcgtagcaat
     3241 gacgcggtaa cttccgttgc ctgaaagtag gcgaatgttg ggaacattcg tttgcgttcg
     3301 ctggtgtcga gagcaggctc aaaaacccgt acctgtccaa acctgcccca gccgttaagc
     3361 cttccctcac gattgcaacg acctgctgcc tgggcaacag cctcaagcgg ggccatggct
     3421 ctatagacca agggaaagtc caaatcaaca cccgcttcca cacattgggt ggaaaccagt
     3481 cggcatggtt gcggattggc agggtctagg cacttgcgca cttgctccag aaccttgcga
     3541 cgatgtgcag tgcaaaggtt ggtcgagagg tggcgcagcc ctgaagtgcc agccatcgct
     3601 ttgagccgtg catgggcatg gcgcttgagg ttgagcacgc acagcacttg tgccgcgtcg
     3661 tctgcgcaca agctatcagc caacgcctgc cagctcaaag actcatcggc tgcgggccac
     3721 tgcacttcaa cccgtttgag gcgcacaaac atggcggcat gacccggcac aatctcggtg
     3781 ggttgccagc cagacgcagc atgctttcga actgcgatat ccaacgtgtc aaaagcgggt
     3841 tgggtggcgg tagcaaacac aacgctgttg ttgtagtcaa gagccaggtg cgatagcgca
     3901 gccaatgtag gcacagccag atgctgaggc aagctctgcg cttcatcgaa aagtatcacc
     3961 gagtcacgta atcgatgcag tttgcgacaa gcggaagatc gattcgagaa caacgactca
     4021 agcaactgaa cattggtggt cagaatgatc ggcgcatccc aattttcggc atacaaccga
     4081 cgttgacgct cagcgtcgtt gcatttgtgc gggtcggact gttctacgcc aacacccgca
     4141 aggctgtggt gttcaaggat gaagatttct ggatcatcag caaacaccgc atgataaatg
     4201 cgcgccgtct gctcgatgat gctcaggtaa ggcacagcca gcaccacacg ttttagtccg
     4261 ttctgtttgg catgcgccaa ggcaaattgc agcatggcca atgtcttgcc ggacccagtc
     4321 ggggcggtca atgagaaaat gcctgtaggc tttatcgccg cttgggtcac agcattccaa
     4381 agctcggcac gggtctgcgc aaccgcgtca cttgatctgt cctgtgacgt tcctcggcga
     4441 accgagagat tcataaacat atcaagtgcc agcactgcac gcgctgcatc caacggtaaa
     4501 cccgtttgcc gatagttctt cccctctgat gtcccattga aatgtgcctc agtgtcgaga
     4561 taatccgcat ccaccaaact cgagaacagc atccgcacat ccagcattcg ggcgatggcc
     4621 tgctgccagg catcgggttg ccccagggga atcattggct tcaatggctg ttcaaacacc
     4681 agaccatcaa cttgtgcacg ttcaagcaaa cggttcagat tggcatcggt caaacgaatg
     4741 ttcggcccca ccggcgacgc accttttagg accataccca atcgctgact cagcgcgtcg
     4801 gaagccgccg cctgcaagcc aatgtgatgc ccttcaatgg ccagtgcagc agccaaagcg
     4861 ccatagggct tcaaggccgc taccgcgcca gcactccaat gatctagccc agcttcttga
     4921 ccccgcaatc tggcttgaaa caaatcgcca tatttgccca agtcatgtaa caagccagcc
     4981 agcactgcct cactttgcca agtcgtccct gcggcaaagt tgccgcccaa atctgaaaca
     5041 cacccaagat gctgtttcag caaatgaagt ttgccgtggt cgttttcact gtgagcaaag
     5101 taggaggtca ttggctattt cactttggtt gtcgacgatt tactttgagg tatcacgcaa
     5161 ctacttcacg gtcaccttgt tcatttaacc caagctttgc ggcgaaagcg cctgccaaat
     5221 gggaaacccc caaaagatgt gcttcgagac tttgagtaat accgtcttct tccctttggt
     5281 gggcaatggg cttattagag cgttgtggtt caggtgaggc catatttggt ggtggaatga
     5341 atttcaagct agcttatcca gccacaggct caccagatga ccctctgccc accccaatca
     5401 aaaaaaatc
//
LOCUS       DAOSLL010000296         5406 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13565_length_5406_cov_4.585124, whole genome shotgun sequence.
ACCESSION   DAOSLL010000296 DAOSLL010000000
VERSION     DAOSLL010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13565_length_5406_cov_4.585124"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /locus_tag="PLB25_12965"
     CDS             <1..1398
                     /locus_tag="PLB25_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HOE42525.1"
                     /translation="AWSANSGLVEFPETMAAPASGSQLLETMEMHDQQAGLVAEEVDD
                     EPEIAQSDNTLVRLINTMIIEAYDRGVSDIHVELPPHKAKVRIRFRKDGILSPYLELP
                     TTYRAAMVARLKIMANLDISERRKPQDGKIDFRKFSAKHALELRVATVPTANGLEDVV
                     MRLLSSAKPVDMGKLGLTAVNLKQLRDAVGRPYGMVLCVGPTGSGKTTSLHSVLSYLN
                     TPERKIWTAEDPIEITQPDLRQVQVNPLIDWTFAKALRSFLRADPDVIMVGEIRDVET
                     AHIAIEASLTGHLVLSTLHTNSAAETVTRLIDMGMNPFNFADSLLAVLAQRLARRLCT
                     ECRSSADAPQAWVDELLDDYLAAFPDELRPGRDDVLAQWKEQYGQQGQLKVFTTTGCS
                     RCLGKGLLGRIGLHELMRVTPGLRRLIQTSARSELVQAEAFKTGEFHTLRQDGILKVL
                     EGHLSIEEVRANSNG"
     gene            1391..2467
                     /locus_tag="PLB25_12970"
     CDS             1391..2467
                     /locus_tag="PLB25_12970"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HOE42526.1"
                     /translation="MAKGTGVASHPYQSLTPDVVLDALASAGWYGDGRQMALNSYENR
                     VYQLHLEDGSAVVAKFYRPGRWSDDQILEEHAFSQELMAAEIPVVGPLGLVGSTLHHF
                     GGFSFSVSPSRGGRAPELDDPDVLEWVGRFLARIHTVGAVKPFAVRPALDLQSFGIDS
                     REWLLAHDKVPLDVQSAWEKASQKAIDLIASSAYCTRATGQKTQNCEADGNAFPGTSI
                     QRIRLHGDCHPGNILWTPLESPAVAGPGPHFVDLDDARSGPAVQDLWMLLSGDRRQQT
                     QQLGTLVDGYEQFRNFDRRELALIEPLRTLRLLHYSAWLARRWDDPSFPINFPWFGSS
                     DYWQGQVLMLQEQAEAMREPPLVV"
     gene            complement(2489..2920)
                     /locus_tag="PLB25_12975"
     CDS             complement(2489..2920)
                     /locus_tag="PLB25_12975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOE42527.1"
                     /translation="MNALINRSSFFDDLLRDFSPAFYVRPLHGDPLPTPAQIKVDVKE
                     NGDSYTVQAEIPGVPKDDIDISLDGNVVSLRAEVKQQDSTSQDDKVLRTERYYGSVSR
                     SFQLPVDIDQAQARAKYDNGVLTLTLPKKTLPSGSQRLTIE"
     gene            2973..3719
                     /gene="xrtH"
                     /locus_tag="PLB25_12980"
     CDS             2973..3719
                     /gene="xrtH"
                     /locus_tag="PLB25_12980"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exosortase H"
                     /protein_id="HOE42528.1"
                     /translation="MSSCHRTCELLANFSRVDGPLFRSNPHPCDANHWQRCASRGGCG
                     RTLLAQLQGPLLAGPFPNGSICSLFAGIIAPMRRFIFIFIVLQLVLFGIELTQLVQQH
                     LVLPWTTLLAQACVAIVTGFDANAAAMGKVLWNPATGFGVSIEPGCNGVEACIILFAA
                     IVAFPSTWKHRAWGLGLGFVAVQALNVVRVISLFYLGQWNLTAFTIAHEYLWQGLIML
                     DVLVVWLLWVRANPDPMADAGTGAATPAAA"
     gene            3738..4367
                     /locus_tag="PLB25_12985"
     CDS             3738..4367
                     /locus_tag="PLB25_12985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42529.1"
                     /translation="MSLQFLWTPPKSLWRFFANAIVLFLLLTPMWMQVSKWTSYPVAG
                     LTQMVLNVGAGYWVRAVNKEPGRIEVETRIQVQVPGSNSSLGVAELVAEADPARYAYG
                     LPLFLALLLAARSKRWWKKALIGYLLLLIPQTFSLVLEILRQIMVAGGRPGALAVDQW
                     QLEAIAIGYQAGSLLLPALAPLALWLWLDQDFFDQVVAQGWRNRRQKGN"
     gene            4709..>5406
                     /locus_tag="PLB25_12990"
     CDS             4709..>5406
                     /locus_tag="PLB25_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOE42530.1"
                     /translation="MLTKTQKTNEALMARRRAAIPRGVGQSHEVFIARGENAEIWDVE
                     GKRYIDFAGGIAVLNTGHCRPEIVEAVKAQIDNFTHTCFQVLAYESYVELAERFNALA
                     PGDFAKKTLFLTTGAEAVENAIKIARSHTKRSAVIAFTSGYHGRTLLTLGLTGKVAPY
                     KTGFGPFPGEIFHAQFPNALHGVSVDDSIASVESVFKNDVEASRVAAIIVEPVQGEGG
                     FNVAPVEFLQRLRTL"
BASE COUNT         1070 a         1549 c         1599 g         1188 t
ORIGIN      
        1 gcctggtcgg caaactcggg cctggtcgaa tttccggaaa ccatggcggc accagccagc
       61 ggctctcagt tgctcgaaac gatggaaatg cacgaccaac aggctggtct ggtcgccgaa
      121 gaagttgacg acgagccaga aattgcccag tccgacaaca ccctggtgcg cctgatcaac
      181 accatgatca tcgaagcgta tgaccgaggg gtgtccgata tccacgtcga gttgccgccg
      241 cacaaggcca aggtccgcat tcgctttcgc aaggacggca tactgtcgcc ttacctcgaa
      301 ctgccgacca cttaccgcgc agccatggtg gcaaggctga agatcatggc caatctggac
      361 atttcagagc gccgcaagcc gcaggacggc aagatcgatt ttcgcaagtt ttctgccaaa
      421 cacgcgctcg aactgcgcgt tgccaccgtg ccgactgcca acggcctgga agacgtggtg
      481 atgcgcctgc tgtcgtcggc caagccggtg gacatgggta aactgggcct gacagcggtc
      541 aatctgaagc agttgcgcga tgccgttggt cgaccctacg gcatggtgct gtgcgtcggg
      601 cccactgggt ctggcaaaac cacgtcgctg cactcggtgt tgagttatct gaacacgcca
      661 gagcgcaaga tctggaccgc cgaagacccg atcgaaatca cccagcctga tttgcgtcag
      721 gtgcaggtta accccctcat agattggacc tttgccaagg ccctgcgttc gtttctgcgg
      781 gccgacccgg atgtcatcat ggttggcgaa attcgcgacg tggagaccgc ccacatcgcc
      841 atcgaggctt cgttgacagg gcatctggtg ctgtcaaccc tgcacaccaa cagcgccgct
      901 gaaacggtaa cgcgcttgat tgacatgggc atgaacccgt tcaactttgc tgactctctg
      961 ctggccgtgc tggcgcagcg gctggcgcgg cgcttgtgta ccgagtgccg cagcagtgcc
     1021 gatgccccgc aagcctgggt ggatgaattg ctggacgact atttggcggc attccccgac
     1081 gagcttcgcc ctggtcgtga cgatgtcttg gcgcagtgga aagagcagta tgggcagcaa
     1141 ggtcaactca aggtgtttac cacaaccggc tgcagccgtt gcctgggcaa gggcttgctc
     1201 gggcgcatcg gtttgcatga attgatgcgg gtgacaccgg gcctgcgccg cctgatccag
     1261 acttcggcac gctccgagct ggtccaggca gaggcgttca agaccgggga atttcacacg
     1321 ctacgccagg acggcattct taaagtgctt gaaggccatt tgagtatcga agaagtacgt
     1381 gccaacagca atggctaaag gcaccggcgt tgccagccat ccctaccagt cgctgacgcc
     1441 cgatgtggtg ctggatgcct tggccagcgc gggctggtat ggcgatggcc gccaaatggc
     1501 tttgaactcc tatgaaaacc gggtttacca gttgcatctg gaagatggca gtgcggtagt
     1561 cgccaagttt taccgccccg ggcgctggag cgatgatcaa attctggagg agcacgcgtt
     1621 ttcacaggaa ttgatggccg ctgaaattcc tgtggtcggg ccgttaggct tggtcggcag
     1681 caccctgcat cactttggtg gtttttcatt cagcgtcagc ccaagccgag gcggacgcgc
     1741 gccggaactc gacgaccccg atgtgctgga atgggtcggg cggtttttgg cacgcatcca
     1801 caccgtgggt gcggtcaaac cgtttgctgt gcggccggcg ctggacctgc agagctttgg
     1861 cattgattcg cgcgaatggt tgctagccca tgacaaggtg ccgcttgacg tgcagtcggc
     1921 ttgggaaaag gcttcccaaa aagctattga tttaatagct tccagcgctt attgcacaag
     1981 ggctacaggc caaaaaaccc aaaactgcga ggcagatggc aacgcctttc ctgggacttc
     2041 catccaacgc attcgcctgc atggcgattg ccaccccggc aatattttgt ggactccgct
     2101 tgaatctccc gctgtggccg gtccggggcc gcattttgtt gatctggacg acgcacgcag
     2161 cggcccggcg gtgcaggacc tgtggatgct gctcagcggc gaccgcagac aacagacgca
     2221 acaactgggc acgctggttg acggctacga gcagtttcgc aattttgacc gccgtgaact
     2281 ggccctgatc gagccgctgc gcaccttgcg cctgctgcac tacagcgcct ggctggcacg
     2341 gcgctgggac gaccccagct ttccgatcaa ctttccctgg tttggcagca gcgactactg
     2401 gcaaggccaa gtgctgatgc tgcaagagca ggccgaggcg atgcgagagc caccgctggt
     2461 ggtttgaccc ggccagcggt gcctgcattt actcaatcgt caggcgctgg ctaccacttg
     2521 gcaaggtctt tttcggcaag gtcaacgtca gcacaccgtt gtcatacttg gcccgggctt
     2581 gcgcctggtc aatatcgacc ggcaactgga agctgcgtga cacggaaccg taataacgct
     2641 cggtgcgcag caccttgtca tcctgactgg tgctgtcttg ctgtttgact tctgcacgca
     2701 acgacaccac attgccatcg agtgaaatgt caatgtcgtc ctttggtaca ccaggaattt
     2761 cggcctggac cgtgtagctg tcaccgtttt ccttcacatc aaccttgatc tgcgccggtg
     2821 ttggcagcgg atcaccgtgc aggggcctga cataaaaagc cggtgagaag tcgcgcagca
     2881 aatcgtcaaa aaagctgctg cggttgatca atgcattcat ttgggcactc ctttcctttg
     2941 cttgcgaggg gaaacccaag gtccccatga ccatgagttc ctgccatcga acatgcgagc
     3001 tgctggcaaa tttttcaagg gttgatggcc cgctttttcg ctcgaatccg catccatgcg
     3061 atgccaatca ctggcaacgg tgtgcaagcc ggggcggttg tgggcgcacc ctgctggctc
     3121 aactgcaggg cccccttctg gctggacctt ttccaaacgg atcgatttgc agtctttttg
     3181 ctggcatcat tgcgcccatg cgccgcttca tcttcatctt catcgttttg caactggttt
     3241 tatttggcat tgagctgaca caactggtac agcagcacct ggtgttgccc tggaccacgc
     3301 tgctggcaca agcctgtgtc gccatcgtca ccgggtttga tgccaacgcg gctgccatgg
     3361 gcaaagtgct ctggaatcct gccaccggct tcggcgtgtc gatcgagccc ggctgcaatg
     3421 gggttgaggc ctgcatcatc ctgtttgctg ccatcgtggc tttcccctcg acctggaagc
     3481 accgggcctg gggcctgggc cttggctttg ttgccgtgca agcgctgaac gtggtgcggg
     3541 tcatcagcct gttttatctg ggacagtgga acctcaccgc tttcacgatt gcacacgaat
     3601 acctttggca ggggctgatc atgctcgatg tgctggtggt ctggctgctg tgggtgcggg
     3661 ccaaccccga tccgatggcg gacgctggca ccggcgcagc aacacctgct gctgcttgag
     3721 gatctggtga gcgacgcatg agccttcaat ttctttggac accgcccaag tcactttggc
     3781 ggttttttgc caacgccatc gtgttgttcc tgttgctcac gccaatgtgg atgcaggtgt
     3841 ccaaatggac ttcttaccct gtagccggcc tgacgcagat ggtgctcaac gtcggggcgg
     3901 gctattgggt acgtgcggtc aacaaggagc cagggcgcat cgaggttgaa acccgcatcc
     3961 aggtccaggt gccaggcagc aactccagct tgggcgtagc tgaactggtg gcagaagctg
     4021 acccggcgcg ctacgcctac ggactgccgt tgtttctggc gctgttgctg gccgcgcgca
     4081 gcaagcgttg gtggaagaag gcgcttattg gttatctgct gttgctgata cctcaaacct
     4141 tcagtctggt gctggaaatt ctgcgacaaa tcatggtggc gggtggcagg cccggtgcac
     4201 tggcagttga ccagtggcag ttggaggcca ttgccatcgg ataccaggcg ggctcgctgc
     4261 tgttgcccgc gctggctccc ctcgcactgt ggctgtggct tgatcaagat ttttttgacc
     4321 aggtggtagc gcaaggctgg cgcaatcgac ggcaaaaagg caattgagcc gaaagtccgc
     4381 aagcgggcgc gttgcgattt ccctctgcaa gtgagcgagg tcagggacgt ttgcgactca
     4441 ggccttgcca cctgacatat cgccggtgca actttcaggt ctgagtcggc acccaaagcg
     4501 gtgaaaggcg taatcattca aattgattga aggtaggagt ggccaatgcg aatccttttt
     4561 caaatcaagg gtttgtctgt attggcagca aagacgacac agtacatcct caaataattg
     4621 cgatctgtat tgggttgaat cgcctcatca tcactatagt cacgcatctt tgccaacccg
     4681 ccgccatttt tcaaggagag aagcgcatat gctgaccaaa acccaaaaaa ccaatgaagc
     4741 cctgatggcc cgccgccgcg ccgccattcc ccgtggtgtc ggtcaaagcc atgaggtgtt
     4801 catcgcacgc ggtgaaaatg ccgaaatctg ggacgtggaa ggcaagcgtt acatcgattt
     4861 tgccggtggt attgcggtgc tcaacaccgg ccactgccgc cccgaaatcg tcgaagccgt
     4921 caaggcgcag attgacaact tcacccacac ctgttttcag gtgctggcgt atgagtctta
     4981 tgtggaactg gccgagcgct tcaatgccct ggcaccgggc gactttgcca agaaaaccct
     5041 gtttttgacc accggtgccg aagccgttga aaacgccatc aaaattgccc gctctcatac
     5101 caaacgcagt gcggtgatcg cgttcaccag cggctaccac ggccgcacgc tgctgacgct
     5161 gggcctgacc ggcaaggtgg caccgtacaa aaccggtttt gggccgtttc ccggtgaaat
     5221 tttccacgcc cagtttccca atgcgctgca tggtgtcagc gttgatgact cgattgcctc
     5281 ggtcgaatcg gtcttcaaga acgacgtgga ggctagccgt gtggcagcca tcatcgtcga
     5341 gccggtacaa ggcgagggcg gttttaacgt ggcaccggtc gagtttttgc aacggctgcg
     5401 tacgct
//
LOCUS       DAOSLL010000297         5403 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13573_length_5403_cov_4.005984, whole genome shotgun sequence.
ACCESSION   DAOSLL010000297 DAOSLL010000000
VERSION     DAOSLL010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5403)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5403)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5403
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13573_length_5403_cov_4.005984"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(239..409)
                     /gene="rpmG"
                     /locus_tag="PLB25_12995"
     CDS             complement(239..409)
                     /gene="rpmG"
                     /locus_tag="PLB25_12995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HOE42531.1"
                     /translation="MATKGGREKIKLESTAGTGHFYTTSKNKKTTPEKLLMKKFDPKA
                     RKHVDYKEMKLK"
     gene            complement(430..663)
                     /gene="rpmB"
                     /locus_tag="PLB25_13000"
     CDS             complement(430..663)
                     /gene="rpmB"
                     /locus_tag="PLB25_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HOE42532.1"
                     /translation="MARVCEVTGKGPMVGNKVSHANNRTKRRFLPNLQYRRFWVESEN
                     RWVRLRISNAGLRLIDKNGIDSVLADLRARGQA"
     gene            complement(845..1792)
                     /gene="trxB"
                     /locus_tag="PLB25_13005"
     CDS             complement(845..1792)
                     /gene="trxB"
                     /locus_tag="PLB25_13005"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HOE42533.1"
                     /translation="MTGNRHTQVLILGSGPAGYSAAIYAARANLSPLLITGMAQGGQL
                     MTTTDVDNWPADVDGVQGPDLMQRFLAHAERFKTEIVFDHINAVDFSKRPFALTGDSG
                     SYTCDALILATGASAKYLGLPSEEAFMGRGVSGCATCDGFFYRNEICCVVGGGNTAVE
                     EALYLSNIAAKVYLIHRRDKFRAEPILIDKLMEKAASGKIVLKTFSTLDEVLGDASGV
                     TGVRIKSVKSGATEDLALKGCFIAIGHSPNTDIFQGQLSMEGGYIITQGGNQGFATQT
                     SVPGIFAAGDVQDHIYRQAITSAGTGCMAALDAQRFLDR"
     gene            1981..4338
                     /locus_tag="PLB25_13010"
     CDS             1981..4338
                     /locus_tag="PLB25_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HOE42534.1"
                     /translation="MTYSLNTLQGSRSAAPSAPRSGLGRFAIELALLVGFVFLLFWLV
                     ALLSYSPQDAAWSTSGTGSLVSNRVGRVGAWLADGSYFLFGFSVWWFPLVGAHVWLTV
                     LAQRLRGQDDPALLSVQPSSVLARFAASRLAFWLGLVMLISASGTLEWTRFYRLEAHL
                     PGHGGGILGYWMGSQGLQRLGFTGSGLLAIVLAMLGAGWVFGFSWNRLAERLGAWLFA
                     RIETRREKIEIKQDVALGQQAAQARADVLVEERQEAAEHAAKPVLLIDPIAVEVPKSP
                     RVAKERQQTLFRDLLDSKLPQVDLLDAALSRQETVSPETLEMTSRMIEKKLKDFGVDV
                     SVVLAQPGPVITRYEIEPATGIKGSQVVGLAKDLARSLSLVSIRVVETIPGKTTMALE
                     LPNAKRQTIKLSEILGSQAYHDAKSLLSMGLGKDIIGNPVLADLAKMPHVLVAGTTGS
                     GKSVGINAMILSLLYKAEANDVRLLMIDPKMLEMSVYEGIPHLLCPVVTDMRQAANGL
                     TWCVAEMERRYKLMSKMGVRNLAGFNTRMDEAKARGELIGNPFSLTPDDPEPLTRLPH
                     IVVVIDELADLMMVVGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIP
                     TRIAFQVSSKIDSRTILDQMGAEALLGMGDMLYMPSGTGLPIRVHGAFVSDEEVHRVV
                     TYLKSQGEPNYIEGILEGGTLEGEEGASGGGGDSGAGEKDPMYDQAVEVVLKNRKASI
                     SLVQRHLKIGYNRAARLVEDMENAGLVSAMSNSGQREILVPKREE"
     gene            4440..5069
                     /gene="lolA"
                     /locus_tag="PLB25_13015"
     CDS             4440..5069
                     /gene="lolA"
                     /locus_tag="PLB25_13015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HOE42535.1"
                     /translation="MKQITTLLIAISAMPVWATGLESLEKFIRTVNTGRATFTQVVTA
                     PARDGQAPRSKASSGSFEFARPNRFKFIYKKPFEQSIVADGQTLWLYDVDLNQVTARK
                     QASALGSTPAALIASAADLQALQADFALTDAPAQDGLEWAVASPKAKDNQLQSVRVGF
                     RGAALEKLEILDSFGQKSVLTFSGFQSNLVPDAAAFHFKPPAGADVIRQ"
     gene            5066..>5403
                     /locus_tag="PLB25_13020"
     CDS             5066..>5403
                     /locus_tag="PLB25_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42536.1"
                     /translation="MSTASLLASASAEKSVQPVGHFVDAQGQSAFRVFAPEKNSVRVV
                     LHDSGKVLALARDPLGFWQGNCERLKHGTLYWLEVDGQKLPDPASRYQPLGILGPSRV
                     TDIGKVHNASA"
BASE COUNT         1049 a         1519 c         1683 g         1152 t
ORIGIN      
        1 tacgctctaa gttgatgatt tttaaaggaa ttttttgggg gttgttaaag accggttaaa
       61 gacgaaagcg aaatgaaagt gaagcccgac aagttgcggc gatttcgcat cggccgcaga
      121 tgaccgggtg catcgtcttt ttgggttcag ttccagcgcc tggctggtgg cactggccga
      181 gttgaggtgg ggcaaaaaaa agcccgcgtc aagcgggctg attgttgcaa aagctaattt
      241 acttcagctt catttccttg taatccacat gcttgcgcgc cttgggatca aacttcttca
      301 tcagcagttt ttctggagtg gtcttcttgt tcttgctggt ggtgtagaag tgaccggtgc
      361 cggctgtcga ttccagcttg attttttcgc gtccgccttt ggttgccatg atgctctttc
      421 taaatgggtt taagcctgac cacgggcgcg caggtctgcg agcaccgagt caataccgtt
      481 tttgtcgatc aaacgcagac cggcattgga aatccgcagg cgaacccagc ggttttcaga
      541 ctcaacccag aaacggcggt attgcaggtt cggcagaaac ctgcgcttgg ttcggttgtt
      601 ggcgtgggaa accttgttcc cgaccatggg gcctttgccc gtaacttcgc atacgcgtgc
      661 catgtggaca ctccgatatt tttaacggcc ccaactggcg ctgggagcct cacctcgcca
      721 agagtagggt ggcggtaacc cgattgccag attgttcccg tcacgggaga atttcagcaa
      781 agcccggcat tatagcgggc gaatcaggcg ggttgtgccg cgcatcgccg ttgcgtcagc
      841 ggccttagcg gtccagaaac cgttgtgcgt cgagtgccgc catgcagccg gtgccagcac
      901 tggtgatggc ctggcggtaa atgtggtcct gcacgtcacc ggcggcaaaa atccccggca
      961 cgctggtctg ggtggcaaag ccctggttgc cgccctgggt gatgatgtag ccgccttcca
     1021 tgctgagctg gccctggaaa atatcggtgt tggggctgtg gccgatggca atgaagcagc
     1081 ctttgagcgc caggtcttcg gtggccccgg atttgacgct tttgatgcgc acaccggtaa
     1141 caccgctggc atcaccgagc acctcgtcga gcgtgctgaa ggttttgagc acaatcttgc
     1201 cactggcggc tttttccatc agcttgtcga tcaagatcgg ctcagcgcga aatttgtcgc
     1261 ggcggtgaat caggtacacc tttgcagcaa tgttggataa gtacagggct tcttccaccg
     1321 ccgtattgcc gccacccacc acgcaacaga tttcattgcg gtagaaaaat ccgtcacagg
     1381 tggcgcagcc tgacacgccc cggcccatga acgcttcttc agacggcagc cccaggtact
     1441 tggctgaagc accagtggcc aggatcagtg cgtcgcaggt gtagctgccg ctgtcaccgg
     1501 tcagggcgaa cgggcgcttg ctgaagtcaa cggcgttgat gtggtcaaag acaatctcgg
     1561 tcttgaaacg ctcggcatgc gccaaaaagc gctgcatcag gtcaggtccc tgcaccccat
     1621 cgacatccgc gggccagtta tcgacgtcgg tggtggtcat cagttggccg ccctgtgcca
     1681 tgccggtgat cagcagcgga ctgaggttgg cgcgggcggc gtagatggcg gcgctgtagc
     1741 cggccgggcc ggagccaaga atcagcacct gggtatgtcg attgcctgtc atttctttcc
     1801 tttatgaatc ggttgcatct ggcgtgtaca gttaggggtg atttgcagca tatcaaccgg
     1861 gcaatcaaaa gcctgaaccg ggtctgcctg cccttgacca tctctatgcc gtcggttggc
     1921 cggattatgc aatgctgctg cgtgtcaacc ttttgtcgtg ttgcctccat ttttttccca
     1981 atgacttact ctttgaatac cttgcaaggc tcacgctccg cggccccgtc ggcaccgcgc
     2041 tcgggtctgg gccgctttgc catcgaactg gcgctgctgg tcgggtttgt gtttttgctg
     2101 ttctggctgg tggccctgtt gagttacagc ccgcaggacg cggcctggtc aacctcgggc
     2161 actggtagtc tggtgtcaaa ccgggtcggc cgggtcggcg cctggctggc cgacggcagc
     2221 tatttcttgt tcggcttttc ggtttggtgg ttcccgctgg tcggagcgca tgtctggctg
     2281 acagtgctgg cccagcgctt gcgtggtcaa gatgatccgg ctttgctcag cgtgcagccc
     2341 agctcggtgc tggcacggtt tgccgcaagc cgactggctt tctggctcgg gctggtgatg
     2401 ctgatcagtg ccagcggcac cttggaatgg actcggtttt accgcctgga ggcacacctg
     2461 ccggggcatg gcggtggcat tttgggttac tggatggggt cgcaaggtct gcagcgactc
     2521 ggttttaccg gttcgggcct gctggcgatt gtgcttgcca tgctgggcgc tgggtgggtg
     2581 tttggcttct cgtggaaccg gttggcagag cggcttggtg cctggttgtt tgcccggatc
     2641 gaaacccggc gcgaaaagat cgagatcaaa caagacgtgg cgctgggcca gcaggcggcg
     2701 caggcgcggg ccgatgtgct ggttgaagag cgccaggaag ccgccgaaca tgcggccaaa
     2761 ccggtgctgc tgatagaccc gattgcggtc gaggtgccga aaagcccgcg ggtcgccaaa
     2821 gaacggcaac agaccttgtt cagggacttg ctcgacagca agttgccgca ggtggacctg
     2881 ctcgatgctg ccttgtcgcg ccaggagacg gtgtcgcccg agacgctgga gatgaccagc
     2941 cgcatgatcg agaaaaaact caaggacttt ggtgtcgatg tcagcgtggt gctggcacag
     3001 cccgggccgg tcattacccg ctatgaaatt gagcccgcca ccggcatcaa gggctcgcag
     3061 gtggtcggcc tggccaaaga tctggcacgt tccctgagcc tggtctcgat ccgcgtggtc
     3121 gagaccattc cgggcaagac caccatggcg ctggagctgc ccaatgccaa gcggcaaacc
     3181 atcaagctgt ccgaaatttt gggttcgcag gcgtatcacg atgccaaatc gctgctgtcg
     3241 atgggcctgg gcaaggacat cattggcaac ccggtgctgg ccgatctggc caaaatgccg
     3301 catgtgctgg tggctggcac caccggctcg ggcaagtcgg tcggtatcaa cgcgatgatt
     3361 ttgagcctgc tgtacaaggc cgaagcgaac gatgtgcggt tgttgatgat cgaccccaaa
     3421 atgctggaaa tgtcggtcta tgagggcatt ccgcatttgc tgtgcccggt ggtcactgac
     3481 atgcgtcagg ctgccaatgg cctgacctgg tgtgtcgccg agatggagcg ccgctacaag
     3541 ctgatgagca agatgggcgt gcgcaacctg gcgggcttca acacccgcat ggatgaggcc
     3601 aaagcgcgcg gcgagctgat tggcaacccg ttcagcctga ccccggatga ccccgaaccc
     3661 ttgacacgcc tgccgcacat tgtggtggtg attgacgaac tggccgactt gatgatggtg
     3721 gtgggcaaaa aaatcgagga actgattgcc cggctggcgc aaaaagcccg tgctgccggc
     3781 attcacctga ttctggccac ccagcgcccc agcgtcgatg tgattaccgg cctgatcaag
     3841 gccaacatcc cgacgcgcat cgcgtttcag gtcagcagca agatcgacag ccgcaccatt
     3901 ctggaccaga tgggtgctga agccttgttg ggcatgggcg acatgctgta catgcccagc
     3961 ggcaccgggc tgccaatccg ggtgcatggt gcctttgtca gcgacgaaga agtgcaccgt
     4021 gtcgtgacct acctcaaaag ccagggcgag cccaattaca tcgaaggcat tctggaaggt
     4081 ggcacgctag aaggcgagga gggcgcgtct ggcggcggcg gtgatagcgg cgctggcgaa
     4141 aaagacccga tgtacgacca ggcggtggaa gtggtgctga aaaaccgcaa ggccagcatt
     4201 tccctggtgc agcggcatct gaaaattggc tataaccgcg ctgcccggct ggtcgaagac
     4261 atggaaaacg ccggcctggt gagcgccatg agcaacagtg gccagcgtga aattttggtg
     4321 cccaagcgtg aggaatgatc gcagtgcccc gaccaacctt gccgcacgca gcagcagcca
     4381 tgccaggtgc agccaggctg gtcgccggcc tccaacctga ccacagcaga atcaactcga
     4441 tgaaacaaat tactacatta ttgatagcta tcagcgcaat gcctgtatgg gctacaggcc
     4501 tggaaagcct tgaaaaattt atcaggacgg tgaacaccgg gcgcgccacc ttcacccagg
     4561 tggtgacagc cccggcgcgc gatggccagg ccccgcgcag caaagcgtcc agtggcagct
     4621 ttgagtttgc ccgaccgaac cgcttcaagt tcatctacaa aaagccgttt gagcaaagca
     4681 tcgtggcaga cggccagacc ttgtggctgt acgacgtgga cctgaaccag gtcaccgcgc
     4741 gcaagcaggc cagcgcgctg ggttcaacac cggccgcatt gattgcttcg gccgccgatt
     4801 tgcaagccct gcaggccgac tttgcgctga ccgatgcccc agcccaggat ggactcgaat
     4861 gggccgtcgc aagccccaaa gccaaagaca accagttgca aagcgtgcgg gtcggctttc
     4921 gcggtgcagc gctggaaaag cttgaaattt tggacagctt tggccagaaa tcggtgctga
     4981 ccttcagcgg ctttcagtcc aatctggtgc ccgatgccgc ggcctttcat ttcaagcccc
     5041 cggccggggc tgacgtgatt cgccaatgag tacggccagc ctcctggcat ctgcatcggc
     5101 agagaagtcc gtgcaaccgg tcggccattt tgttgacgcg caaggccaat cggcctttcg
     5161 tgttttcgcc ccggaaaaaa acagtgtccg ggtggtcctt catgattcgg gcaaggtgct
     5221 ggcgctggca cgcgatccgc tcgggttttg gcagggcaat tgcgagcggc tcaagcatgg
     5281 caccttgtac tggcttgaag ttgatggcca aaaattgcct gacccggcat cgcgctacca
     5341 gcccctgggc attctcgggc cgtcccgggt gactgacatt ggcaaggttc acaacgcctc
     5401 ggc
//
LOCUS       DAOSLL010000298         5397 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13585_length_5397_cov_4.417634, whole genome shotgun sequence.
ACCESSION   DAOSLL010000298 DAOSLL010000000
VERSION     DAOSLL010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13585_length_5397_cov_4.417634"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            138..422
                     /locus_tag="PLB25_13025"
     CDS             138..422
                     /locus_tag="PLB25_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008443150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HOE42537.1"
                     /translation="MRTVTEAKRETLNIRIKPEVRDLIDRAAKSRGKNRTDFILDSAR
                     LAAEDALLDQVIMSVSLQAFEQFQTRLDMPPNANDRLRKTMLTPAPWERE"
     gene            419..931
                     /locus_tag="PLB25_13030"
     CDS             419..931
                     /locus_tag="PLB25_13030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE42538.1"
                     /translation="MKLTAPTPLTEHHVTEGFSCGIHSLDQWLIRRSLKNQMQGASRT
                     YVVCDETRVVAYYALASGALTTTDATGRFRRNMPEPIPVVVLGRLAVDKTLHGKGFGQ
                     SLVRDAGLRVIQAADAIGIRGMTVHALSDDAKTFYEKIGFEPSPIDSHLLMITLPDLM
                     DACGSVPQSL"
     gene            complement(1526..4495)
                     /locus_tag="PLB25_13035"
     CDS             complement(1526..4495)
                     /locus_tag="PLB25_13035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HOE42539.1"
                     /translation="MSSIHETAYPRFKSEITPRELADVYTPSPEEQAFARRQGRTLQG
                     RLAVLVLVKTAQRLGYFVKLAEVPPSIVVHIATCLATKPMTKAEMRDFDLNSERQHQR
                     LLEQVRRFLGIQAVTGATTEVIESTAREAAQTKHELADIINVVIEQLIRQRFELPGFS
                     TLLRTARRIRSQVNDELFRLLSGALSPALKHEIDDLFALKTGETSGWLKLKRESKKPT
                     NPQVRAYLEHLAWLKAWVARLPDIDHIAAPKLHQYVLEARALDAADIKATKLAKRYAL
                     AVVLIHAQLRQALDDVVDILLRKLRKLHATGAEQLERYFTEHRLRAEKLIATLRDMLK
                     AFEAGTTDTERGEHIARAIEGDSAQLLAQCDEHMAYAADNYFPFMMASYQAQRPLLLN
                     CLSLLELASTHSDQTLVQAIAFVLQHRNSHKEHLPVDQAALSLNWLPDKWRRIVVQEN
                     QGDEGSCVHRKYFELCVLSEVTRELQSGDLYVRRSEQYGDYREQLIDWDTFESQVADY
                     GAMLALPTEAGAFVAQLRQRLVDTAKRVDQAFPNNEHVAFVGEELVIRRNSKEASSAA
                     LLRVDQELCARLAPKNILDILVEGEHWLNLHKLFGPLSGFESKIDNPRLRFVTTLFCY
                     GCNLGPTQTARSVKDLSRKQVAWLNLHHMTEERLEKALVQVINAYNKFLLPKYWGSGE
                     TASADGTQWNLYEQNLLAESHIRYGGYGGIGYYHVSDKYIALFSHFIPCGVYEAVYIL
                     DGLIKNESDVQPDTVHGDTHAQSTPAFGLAYLLGIKLMPRIRQLKKLVFFRPDKKTRY
                     RHIDALFSESIDWSLISTHLPDMLRVALSIKAGKITPSALLRRLGTASRKNKLYFAFR
                     ELGRVVRTQFLLDYIGDVELRRTIHAATCKSEEFNQFTKWLFFGGEGVIAENMRHEQR
                     KAIKYNQLVANLVILHNVEAMTRVLKEIQADGFVVDAAVLGGLAPYRVEHINRFGDYS
                     LDLERQVQPMQFETILI"
     gene            4691..5233
                     /locus_tag="PLB25_13040"
     CDS             4691..5233
                     /locus_tag="PLB25_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOE42540.1"
                     /translation="MLAFAVKAGFDVVGVWKETASGTKDDRAQRKKVMALAQARDVDV
                     VLVTELTRWGRSTIDLFHTLGELQAWGISLIAQTGLQFDLGTPQGKLIASLMSVLAEF
                     ERDLLRERVRSGIASAQARGVRFGRQTGQRVKADRHQKRALELVAAGQSYREIGLRLG
                     ISKNTVLDIVKRHRAEQAGQ"
BASE COUNT         1119 a         1515 c         1552 g         1211 t
ORIGIN      
        1 tgcgcctggc caagaaaccg cctgaataac ggggagaact tggcactgtc cagaagttta
       61 aaaatgcata cggcaattaa ctaatgacaa tgtcattaca atctggtaaa atcgacacat
      121 accaaggaga aatcctcatg cgcactgtta ccgaagcaaa acgcgaaact ctcaatatac
      181 gcataaaacc cgaagtccgc gatctgattg atcgtgctgc gaagtcacgt ggcaagaatc
      241 gcactgactt tatcctggac tctgcccgtc ttgccgcaga agacgcgttg ctcgaccaag
      301 tgatcatgtc ggtcagtctg caagcatttg agcagtttca gactaggttg gacatgcccc
      361 ccaatgccaa cgaccgttta cgcaaaacca tgcttactcc cgctccctgg gaacgtgaat
      421 gaagttgacg gctccgacgc cgttgacaga gcatcatgtg acagaaggat tttcatgtgg
      481 catccacagc ttggatcaat ggctcattcg gcgaagcttg aaaaatcaga tgcaaggtgc
      541 atcacgaacg tatgtggttt gtgatgagac ccgtgtagtg gcctactatg cgctggcatc
      601 tggcgcgctc accacgaccg atgccaccgg tcggtttcgc cgcaatatgc cagagcccat
      661 tcccgtcgtc gttttgggcc gtttagcagt tgacaagacc ttgcatggta aagggttcgg
      721 tcagtcgctg gttcgggacg ctggcttgcg cgtcatccag gcggctgatg ccattggtat
      781 ccggggaatg acagttcacg ccctgtcgga cgatgcaaaa accttctacg agaaaattgg
      841 ttttgaaccc tccccaattg attctcacct gctgatgatt accttgccag acctcatgga
      901 cgcatgtggc tcagtgccgc agtccttatg atttttggcc agttcatcca atctgagtgt
      961 tcacgtgcca cctcgctcgg gtgtcgctgc ggcaccatga cctccattcc gcgaagcacg
     1021 cccagaatac cattcgtcga gaagcttcat ccccattgcg aactcaattt cgaggtaatg
     1081 accacctact ggccactagg ccgcgtcaag ggttcgcttc gccaggccgc tcacgcggcc
     1141 tgtccttgac taggctacgt ggccaaagcc gcatatttcg tgcccgaaaa aaccctgccg
     1201 ccagatttac gcgcttttcg ataaccgttc acaactggtg aggtttctca gcggaatttt
     1261 ctggggttcc ggctgacaac tccagatcgc gcagaatgta ggcatagacc ttgtgctcag
     1321 ggtggggctt gctggtgttg gacttgggtc ccaccttaaa ttgttgcaaa ctggttttag
     1381 ccggttgcac ggggatgttg cttgtccggc tgtttgtgtc attctttccc tactgccgtt
     1441 tgccggtagg caggatgtcg cccgaacgtg agggtcggcc gggattcgga cggaacccga
     1501 caaatcttga tgtaagcgac tgatttcaaa taagaattgt ctcgaattgc atgggctgaa
     1561 cctgccgctc caagtccagg gagtaatctc caaatcgatt gatgtgctcg acccggtagg
     1621 gagccaggcc gcccaggaca gccgcgtcaa cgacaaatcc atcagcctga atctccttga
     1681 gcacgcgggt catggcttcc acgttgtgca aaatgaccag gttggccacc aactggttgt
     1741 atttgatggc cttgcgctgc tcgtggcgca tgttctcggc aatgacgcct tcgccgccaa
     1801 agaacagcca cttcgtgaac tgattgaact cctcgctctt gcaggttgcg gcgtgaatcg
     1861 tgcggcgcag ctccacatcg ccgatgtagt ccaacaggaa ctgggttcga accacccgtc
     1921 cgagctcgcg aaaggcaaag tagagtttat tcttgcggct ggccgtgccc aggcggcgca
     1981 gcagcgccga cggtgtgatc tttcccgcct tgatcgacaa ggcgacgcgc aacatgtctg
     2041 gcaggtgggt tgaaatcagc gaccagtcga tgctttcgct gaacagcgcg tcgatgtgcc
     2101 gataccgggt tttcttgtcg ggccggaaga acaccagctt tttgagctgg cgaatcctgg
     2161 gcatcagctt gattcccagc agataggcca gaccgaaggc cggtgtgctc tgcgcatggg
     2221 tgtcaccatg caccgtatcg ggctgcacgt cggactcgtt cttgatgagc ccatcgagaa
     2281 tataaaccgc ctcgtagacg ccgcacggaa tgaagtggct gaagagggcg atgtacttgt
     2341 ccgagacatg gtagtaaccg atgcccccat agccgccgta gcggatatgg ctttcagcca
     2401 gcagattctg ctcgtacaga ttccactggg tgccatcggc ggaggccgtc tcgccgcttc
     2461 cccagtattt tggcaacagg aacttgttgt aggcgttgat gacctgcacc aaagcttttt
     2521 ccagccgttc ctcggtcata tggtgcaaat tcagccaggc gacctgcttg cggctcaaat
     2581 ccttaaccga gcgggccgtt tgcgtgggac ccaaattgca cccgtagcaa aacagcgtgg
     2641 tgacaaagcg cagcctggga ttgtcgatct tgctctcaaa gccggacaag gggccaaaca
     2701 gcttgtgcaa attgagccag tgctcgcctt cgaccagaat atccaggatg ttcttcggtg
     2761 ccagacgggc acagagctct tggtccactc gcagcaaagc tgcagagctt gcttctttcg
     2821 agttcctgcg gatgaccagc tcttccccga caaaggcgac atgctcattg ttgggaaagg
     2881 cttggtccac ccgcttggcc gtgtccacca aacgctgcct gagctgcgcg acgaaagcgc
     2941 ccgcctctgt tggcagtgcc agcatggcgc cgtagtcggc cacctgggac tcgaacgtat
     3001 cccagtcgat gagttgttcc cggtagtcgc cgtactgctc gctgcgccgg acatacaaat
     3061 cgccggactg cagctcgcgt gtcacttccg acaatacgca caattcgaaa tacttgcggt
     3121 gaacgcaact gccttcatcc ccctggtttt cttggacgac gattcgccgc catttgtccg
     3181 gcagccaatt caggctgagt gccgcctggt cgacgggcaa gtgctctttg tggctgttgc
     3241 ggtgctggag cacaaaggca atcgcctgca ccagcgtctg gtcgctgtgg gtcgaggcca
     3301 attccagcag gctcagacag ttgagaagaa gcgggcgctg cgcttggtag gacgccatca
     3361 tgaaaggaaa ataattgtcc gcggcataag ccatgtgctc gtcgcattgc gccagtagct
     3421 gcgccgaatc gccctcgatg gcccgggcga tgtgttcgcc gcgctcggta tcagtggtgc
     3481 ctgcctcgaa ggctttgagc atgtcgcgca gcgtggcaat gagcttttct gcccgcaggc
     3541 ggtgctcggt gaaatagcgc tccaactgct cggcgcccgt ggcatgcagt tttcgcaatt
     3601 tgcgcagcaa aatgtctaca acatcatcca gtgcctggcg caactgcgca tggatcagta
     3661 ccacagccaa cgcgtagcgc ttagccagtt tggtcgcctt gatgtccgcc gcgtccagcg
     3721 cacgtgcttc cagcacgtac tgatgaagtt tcggcgccgc aatgtggtcg atatcgggca
     3781 ggcgagccac ccaggccttc agccatgcca gatgctccag ataggcgcgc acttgcgggt
     3841 ttgtcggttt tttggattcg cgtttgagct tgagccagcc cgaggtctct cccgtcttga
     3901 gtgcgaacaa gtcgtcgatt tcatgtttca atgcaggcga tagcgcacca gacagaagac
     3961 ggaacaactc gtcattgact tgcgagcgaa tgcgccgcgc tgtgcgcaac aaggtgctga
     4021 accctggcag ttcgaaacgc tggcggatta actgctcgat caccacgttg atgatgtcgg
     4081 ccagttcgtg tttggtctgg gccgcttccc gggccgtcga ttcgatgact tcggtggtgg
     4141 cgccagtgac ggcctggatg ccgaggaaac ggcgcacttg ttcgagcagt cgttggtgct
     4201 ggcgctcact gttcagatcg aaatcgcgca tttcagcctt cgtcatcggt ttcgtcgcca
     4261 ggcaagtggc gatatggaca acaatgctgg gcggcacctc ggccagcttg acaaagtagc
     4321 ccagacgttg cgccgtcttg acgagcacca ggacggccag acggccttgc agcgtacgcc
     4381 cctgtcgacg ggcaaacgcc tgttcttctg gggatggcgt gtaaacgtcg gcgagttcgc
     4441 gcggcgtgat ctcggacttg aagcgagggt aggcggtttc gtgaatactg ctcacgaggt
     4501 ctgccctgca aaaaaaatca ccgaaccgca tcatcccatt taacctatac tttatttgga
     4561 ccgccattct gccacgacag catgcgggtt tgcgggtggt tttgcgcgaa ggattcaatt
     4621 gggacagcga gctgccattt actgccgtgt ttccacggcc gaccagactt gtgagcgaca
     4681 agaggttgac ttgttggcgt ttgccgtgaa ggcgggattt gacgttgttg gtgtctggaa
     4741 ggaaacggcc tcgggcacca aggatgatcg cgcccagcgc aagaaggtga tggcactggc
     4801 ccaggcgcgc gatgtcgacg tggtcctagt caccgagctg acacgctggg gccgctcgac
     4861 gatcgatttg tttcacacgc tgggtgaact tcaggcatgg ggcatttccc tgattgccca
     4921 aacaggtctg caatttgatc tcggcacgcc gcagggaaaa cttattgcat cgctgatgtc
     4981 cgttctggcc gagttcgagc gggatttgct gcgcgaacgg gtgcgctctg gcattgcttc
     5041 agcgcaagcg cgcggcgtac gctttgggcg ccagaccggc cagcgcgtca aggccgatcg
     5101 ccatcaaaag cgcgcgcttg agctggtggc ggcgggccag tcttaccgcg aaattggcct
     5161 gcgcttgggc atcagtaaga acacggtgct ggacatcgtc aagcgccatc gcgcagagca
     5221 ggcagggcaa tagcgttcag aggtcgaccg gggcaatgct cgccttgaaa tagaaccatt
     5281 tcaagcactt ttattcaatt gaaacaacag cttggggtga ataatgtcgg gtttcgtccg
     5341 aatcccggcc gaccctcaag acggccaccg ccgtgtaccc gggcataggc tggatcg
//
LOCUS       DAOSLL010000299         5385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13616_length_5385_cov_4.930957, whole genome shotgun sequence.
ACCESSION   DAOSLL010000299 DAOSLL010000000
VERSION     DAOSLL010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5385
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13616_length_5385_cov_4.930957"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            16..801
                     /locus_tag="PLB25_13045"
     CDS             16..801
                     /locus_tag="PLB25_13045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HOE42541.1"
                     /translation="MIIIGDEILSGKRADKHLPQVIELLKARGLQLDYAEYVGDAPER
                     ITAALQRAFESGDVVFSTGGIGATPDDHTRQCAARALGVPLALHPQAKELIEQRMHGL
                     AREKGKVFDPAAPDSRHRLNMGVFPQGADIIPNPYNKIPGFSCQRVHFVPGFPVMAWP
                     MIEWVLDTCYGAFFRTKAWIEQSIILLGAMEATLTPLMEQIESEHPTVKVFSLPSVDH
                     PEYGRHVELGVKGEPAAVAAAYATLLAGLTRFNLKLGPELVRN"
     gene            1033..2448
                     /gene="glnA"
                     /locus_tag="PLB25_13050"
     CDS             1033..2448
                     /gene="glnA"
                     /locus_tag="PLB25_13050"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HOE42542.1"
                     /translation="MAKTVADVMKMVKDNEVKFVDFRFTDTRGQEQHVTVPVSHFDED
                     KFTSGHAFDGSSVAGWKGIEASDMQLMPDPNTANIDPFYEETTIFMSCDVIEPSDGKA
                     YERDPRSVARRAEAYLKASGMGDTAYFGPEPEFFLFDDVRWNTDMSGCFFKIDDYEAP
                     WNSGKKLEGGNRGHRPTTKGGYFPVPPVDSTQDMRAEMSLILESLGIPVEVFHHEVAS
                     PGQCELGTKFSTLVERADWTQVLKYVVQNVANTYGKTATFMPKPIVGDNGSGCHVHQS
                     IWKDGKNLFAGDGYAGLSDFALYYIGGIIKHARALNAITNPGTNSYKRLVPGFEAPVK
                     LAYSAKNRSASIRIPYVANPKGRRIEARFPDPLMNPYLGFSALMMAGLDGIENKIHPG
                     EAATKDLYHLPPEENALIPTVCHSLDQALECLDADRSFLTKGGVFTDGLLDAYIELKM
                     TEVTRLRMATHPIEFDMYYSL"
     gene            2778..3302
                     /locus_tag="PLB25_13055"
     CDS             2778..3302
                     /locus_tag="PLB25_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42543.1"
                     /translation="MKRLFFLCTLVGAVALPTVAQEAIYRCGNEYTNTKPDAKAHGCK
                     RIEGGNVTVVQAVQAPKGAVSKASSAGSAVPGGGNRVVSVEQKARDSDARLILQSELK
                     KAQARQEELQKEYNHGEPEKRGDEARNQQKYLDRVAELKASLARNESDVAGIRRELER
                     LPGASAASGAASSR"
     gene            3373..4407
                     /gene="glnL"
                     /locus_tag="PLB25_13060"
     CDS             3373..4407
                     /gene="glnL"
                     /locus_tag="PLB25_13060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HOE42544.1"
                     /translation="MAFDLLATSVAVVRSDGTVLYVNAALEDARGLSRRVLVGSHLPD
                     SFTQPSHLSTALQGAGDREFPPLRYDAWLLRMGREPLPVFVIVSPTQGPELMVEMQPL
                     EQQARKDREERLAEQAQANKELIRNLAHEIKNPLGGIRGAAQLLEMEMDSAELTEYTQ
                     VIIREADRLQSLVDRLLAPHRRPHVVGDVNIHEICERVRSLILAEFPRGLVVVQDYDT
                     SIPEFRGDQEQLHQALLNVARNACEALTDRIAKGDARLVFRTRVARQATFGRQRYRLA
                     LELHVIDNGPGVPDSIKDRIFYPLVSGREGGSGLGLTLAQNFVGQHHGLIECDSVPGR
                     TDFKIQIPLP"
     gene            4476..>5385
                     /locus_tag="PLB25_13065"
     CDS             4476..>5385
                     /locus_tag="PLB25_13065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOE42545.1"
                     /translation="MKPIWIVDDDQSIRFVLEKALLREDLPTRSFTNARDVLNALSSE
                     EEKGPQVLVSDIRMPGGSGLDLLDKVKVRFPGLPVIIMTAYSDLDSAVSAFQGGAFEY
                     LPKPFDIPKAVELIRRALEESQREEVSEERISDAPEMLGQAPAMQDVFRAIGRLSQSN
                     VTVMITGESGSGKELVARALHKHSPRANGPFVAINTAAIPKDLLESELFGHERGAFTG
                     AQTMRRGRFEQADGGTLFLDEIGDMPFDLQTRLLRVLSDGHFYRVGGHGAVKTNVRVI
                     AATHQNLEQRVKDGGFRHEGVDLVTGQ"
BASE COUNT         1114 a         1532 c         1609 g         1130 t
ORIGIN      
        1 gcccccaagt ttggcctgat catcattggc gacgaaatcc tgtcaggcaa gcgcgccgac
       61 aagcacctgc cgcaggtgat tgagctgctc aaggcgcgtg gcctgcagct tgactatgca
      121 gagtatgtgg gtgatgcacc cgagcgcatt accgccgcgt tgcaacgcgc ttttgagtcg
      181 ggcgatgtgg ttttttccac cggtggtatc ggggccacac cagacgatca cacccgccag
      241 tgtgcggcgc gggcgctcgg tgtgccgctg gcactgcacc cgcaggccaa agagctgatc
      301 gagcagcgca tgcatggact tgcgcgagaa aaaggcaagg tgtttgatcc cgctgcaccg
      361 gacagccgac accggctcaa catgggcgtt ttcccgcaag gtgctgacat catccccaac
      421 ccctacaaca agattcccgg cttcagttgt cagcgggtgc attttgtgcc gggctttccg
      481 gtgatggcct ggccgatgat cgaatgggtg cttgacacct gttacggtgc atttttccgg
      541 actaaagcct ggattgagca gtcgatcatt ctgttgggtg ccatggaggc gacgctgacc
      601 ccgctgatgg agcagatcga gtctgagcac ccgacggtca aggtgtttag cctgccgagc
      661 gtggaccacc cggaatatgg ccgtcacgtt gaactcggtg tcaaaggtga gcctgcggcg
      721 gtcgcagcag cgtatgccac gctgttagct ggtttgaccc ggtttaatct caagctaggc
      781 cccgaattgg tgcgaaattg agcttttcct tttggtgcat tcattagtgc cttattttgg
      841 tgccaccctc attgagccag gtgacgcagc ggggtctgct gcgcatgcgg ctcgatcatg
      901 atggcggtca ctttcaaagt cacagcaaaa aataacttag ggcagggcga cctgaaaccg
      961 tcgttctggc acaaaaactg catccaagat cattgaatct tttttttaca acgacccaac
     1021 caggagtatt tgatggccaa gaccgtcgca gatgtcatga agatggtgaa agacaacgaa
     1081 gtcaagtttg ttgacttccg tttcaccgat acccgtggcc aggaacagca cgtcaccgtg
     1141 cctgtttcgc attttgatga agacaagttc acctcgggcc acgcgtttga cggttcctcc
     1201 gtggcgggct ggaagggcat tgaagcctct gacatgcagc tcatgccaga ccccaatacc
     1261 gccaacatcg atccctttta tgaagaaacc accatcttca tgagttgcga cgtgattgag
     1321 cccagcgatg gcaaggcgta tgaacgtgac ccacgttcgg tagcccgccg ggccgaagcc
     1381 tacctgaagg cctccggcat gggcgatacc gcctattttg gtcccgagcc agaattcttc
     1441 ttgtttgatg acgtgcgctg gaatactgac atgtcgggct gtttcttcaa gattgacgat
     1501 tacgaggcac cctggaactc gggcaagaag ctcgaaggcg gcaaccgtgg tcaccgtccc
     1561 accaccaagg gtggctattt cccggtgcct ccagtcgaca gcacgcagga catgcgcgcc
     1621 gaaatgtcgc tgattcttga atctctgggc attccggttg aagtgtttca ccacgaagtc
     1681 gcatctcctg gtcagtgcga gcttggaacc aagttcagca ccctggttga gcgcgctgac
     1741 tggactcagg tcttgaaata cgtggttcaa aatgtcgcca acacttatgg caagaccgcc
     1801 accttcatgc ccaagccgat cgttggcgac aacggcagcg gttgccacgt gcaccagtcg
     1861 atctggaaag acggcaaaaa tttgtttgct ggcgacggtt atgccggttt gagcgacttt
     1921 gcgctgtact acattggcgg catcatcaag catgcccgtg cactcaacgc gatcaccaac
     1981 cccggcacca acagctacaa gcgcctggtg cccggttttg aagcgcccgt gaagctggct
     2041 tactcggcca agaaccgttc ggcctcgatc cgcattcctt acgtggccaa ccccaagggc
     2101 cgtcgcattg aggcacgctt ccccgatccg ctgatgaacc cctacctggg cttctcggcg
     2161 ctgatgatgg cgggcctgga cggcattgaa aacaagatcc atccgggcga agctgctacc
     2221 aaggatttgt accatctgcc acccgaagaa aacgcgctga tcccgaccgt ttgccacagt
     2281 cttgatcagg ccctcgaatg tctcgatgct gatcgcagct tcctgaccaa gggtggtgtg
     2341 ttcaccgacg gcctgctcga tgcctacatt gaactcaaga tgaccgaagt cacgcgcctg
     2401 cgcatggcga cccatccgat tgagtttgac atgtactatt cactgtaatc agtgagcggc
     2461 gcgcgcctgc tgtgattgac acagccaggc gcgtgtcagg cgtatggcgc attggctgtg
     2521 aaccggtcgt agcggcccaa tttgcacctt cgttttgatc catggcgggg ctatggacct
     2581 gcgaatccgg cacaaattgg tctcatcggg tcaatccgtg atcgatgcca taagtcggac
     2641 aggccccttg ggcttattga tgaatgatga cgacttcggt cgtctttttt tattgtggga
     2701 aactactgtt ggcccgtcac cgtgttgatc gttgttgttg caacacggcg tgatggcctg
     2761 aaaagtgtgg aactccaatg aagcgattgt tttttctgtg taccttggtg ggtgcagtgg
     2821 ctctgccaac cgtggcccag gaagccattt accgctgcgg caatgaatac accaatacca
     2881 agcccgatgc caaagcccat ggctgcaagc gcattgaggg tggcaatgtc accgtggtgc
     2941 aggcggttca ggcccctaaa ggcgcagtgt cgaaagccag ttcagcaggc agcgcggtgc
     3001 caggtggcgg caaccgcgtc gtatcagtgg aacaaaaagc ccgtgattct gacgcccgcc
     3061 tgattttgca gtccgagctg aaaaaggccc aggcacgcca ggaagagttg cagaaggaat
     3121 acaaccatgg cgagcccgaa aagcgcggtg atgaagcgcg caaccagcag aaatacctcg
     3181 accgggtagc cgagctcaaa gccagcctgg cgcgcaacga aagcgatgtg gcaggcatcc
     3241 ggcgcgagct tgagcgcttg cctggtgcat ccgcagcatc tggtgcagca agttcgcgct
     3301 gatgacccgg actgaactgt gaagccaact gatttgactg gaacctcacc gcgtgcggcg
     3361 gcagagcgct tcatggcgtt tgatctgctg gccacttcgg tggcagtggt ccgcagcgac
     3421 ggcacggtgc tgtatgtcaa tgccgcgctg gaggacgcac gtggcctttc cagacgggtc
     3481 ctggtaggct cgcatttgcc cgacagtttc acccagccga gtcacttgag cacagctttg
     3541 cagggtgcgg gtgaccgtga atttccgccc ctgcgctacg atgcctggct attgcggatg
     3601 gggcgcgagc cgctgccggt gtttgttatc gtctcaccca cccaaggccc ggaactgatg
     3661 gtcgagatgc agccgctgga gcagcaggcc cgaaaagacc gcgaagagcg tctggccgaa
     3721 caggctcagg ccaacaagga actgattcgc aacctggcgc atgaaatcaa aaacccgctc
     3781 ggcggcattc gtggcgcggc ccagttgctg gaaatggaaa tggattcggc ggaactgacc
     3841 gagtacaccc aggtcatcat ccgcgaggcc gatcgtctgc agtcgctggt ggaccgcttg
     3901 ctggcaccac accgtcggcc gcacgtcgtg ggcgatgtga acattcacga gatttgcgag
     3961 cgggtgcgct cgttgatcct ggccgagttt cctcgtgggt tggtggtggt gcaagactac
     4021 gacacctcta ttccagagtt ccgtggtgat caggagcagc tccaccaggc cctgctcaat
     4081 gtggcgcgaa acgcctgcga ggccctgacc gaccgcattg ccaagggtga tgcccggctg
     4141 gtatttcgta cccgggttgc gcgccaggcc actttcggtc gacaacgcta ccggttggca
     4201 ctggaattgc atgtgatcga caacgggcct ggtgtaccag actcgatcaa ggaccgtatt
     4261 ttttatccac tggtgtccgg gcgcgaaggt ggctccgggc tgggcctgac attggcgcaa
     4321 aactttgtcg ggcagcacca cggcttgatt gagtgtgaca gcgtgccggg acgtactgat
     4381 ttcaaaatac agattccact accctgaccg gggcagcgca ctgcaagggg gcagtgtctg
     4441 gctagtccgg atgcaaccaa aggtgacaag acacgatgaa acccatttgg atcgttgacg
     4501 atgaccagtc cattcgtttc gtgctggaga aggcgttgct gcgcgaagac ctgccgaccc
     4561 gcagtttcac caacgcacgc gatgtgctca atgccctgtc cagcgaggaa gaaaaggggc
     4621 cgcaggtgct ggtcagcgac attcgcatgc cgggtggctc cgggctggac ctgctcgaca
     4681 aggtcaaggt ccggtttcct ggcctgccgg tgatcatcat gaccgcttac tcggaccttg
     4741 acagtgccgt gtcggcgttt cagggtggtg cgtttgagta cctgcccaag ccgtttgaca
     4801 ttcccaaggc ggtggagctg attcgccgcg cactggagga aagccagcgc gaagaggtca
     4861 gcgaagagcg catcagcgat gcgcccgaga tgttgggcca ggccccggcc atgcaggatg
     4921 tgtttcgcgc cattggccgc ctgagccaga gcaacgtcac ggtcatgatc accggcgaat
     4981 ctggctctgg caaagaactg gtggcgcgcg ccctgcacaa gcactcgcca cgcgccaacg
     5041 ggccatttgt tgcgatcaat accgcagcca tccccaaaga cctgctcgaa tccgaactgt
     5101 ttggccacga gcggggcgct tttacgggtg cccagaccat gcgccgtggc cgttttgagc
     5161 aggccgatgg cggcaccctg tttctcgacg agattggcga tatgccgttt gatttgcaaa
     5221 cccggctgct gcgggtgctg tcagacgggc atttctaccg ggtaggcggc catggcgcag
     5281 tcaagaccaa tgtgcgcgtg attgccgcta cccaccagaa cctggaacag cgcgtgaaag
     5341 acggcggttt ccggcatgaa ggcgttgatc tcgttaccgg acagg
//
LOCUS       DAOSLL010000300         5369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13661_length_5369_cov_4.780956, whole genome shotgun sequence.
ACCESSION   DAOSLL010000300 DAOSLL010000000
VERSION     DAOSLL010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13661_length_5369_cov_4.780956"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /gene="tsaD"
                     /locus_tag="PLB25_13070"
     CDS             complement(<1..535)
                     /gene="tsaD"
                     /locus_tag="PLB25_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HOE42546.1"
                     /translation="MQVLGIESSCDETGVALVEVRGPGVPVLLAHALYSQIEMHQAYG
                     GVVPELASRDHIRRVLPLTEQVLAESGRTLADVDVLAFTRGPGLAGALLVGAGVACAL
                     AAALGKPVLGVHHLEGHLLSPFLSADPPEFPFVALLVSGGHTQLMQVDGVGRYQIMGE
                     TIDDAAGEAFDKSAKLLG"
     gene            complement(1065..2219)
                     /locus_tag="PLB25_13075"
     CDS             complement(1065..2219)
                     /locus_tag="PLB25_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid 1,2-hydratase"
                     /protein_id="HOE42547.1"
                     /translation="MPNPSALQLPRRQLLTRALLAPLWPGLAWLQVSRAAAATLVKRE
                     LVFARDLGSHPDTAIEWWYVTGELQAKQQQYGFQLTFFRSRVPATQHMTSAFAARQLI
                     FAHAAVTDLSGQRLLHDQRIARSSGTASVDLAEASEADTHIRLRDWSLQRREDGRYLA
                     RVTAQDFAFALDLTPTQPLLLQGEQGWSRKGPQATQASFYYSLPQLKLAGDLTLAGST
                     RRVSGRAWLDHEWSDALLHPEAVGWDWIGMNLFDGAALTAFRLRNKAGATLWAGGSWR
                     GGGAAKPVVFAAQDVRFKAGRIWKSPASGASYPVQWTIDLPAAAIDAGLPARTSFTVR
                     ALLDAQELDSRSSTGAIYWEGLSELSNQSGQPIGRGYLEMTGYASPLKLS"
     gene            complement(2464..3243)
                     /locus_tag="PLB25_13080"
     CDS             complement(2464..3243)
                     /locus_tag="PLB25_13080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE42548.1"
                     /translation="MTHTTPLKLQVENIHKRFGSNEVLKGVSLTAHAGDVISIIGSSG
                     SGKSTFLRCINLLEKPHLGRIHVVDEELHLTAAPNGELHAANPKQLARLRTKLAMVFQ
                     HFNLWAHMTVLQNIIEAPVHVMKVPRDQAIAIARKYLAKVGLEGKEDAYPAHLSGGQQ
                     QRVAIARALAMEPEVMLFDEPTSALDPELVSEVLKVMKNLALEGRTMVVVTHEMGFAR
                     EVSNHLIFLHKGQIEEEGNPTQVLSNPKSVRLAQFLSGSLK"
     gene            complement(3275..4405)
                     /locus_tag="PLB25_13085"
     CDS             complement(3275..4405)
                     /locus_tag="PLB25_13085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HOE42549.1"
                     /translation="MQRLDHPLLSNSLGSQKTITSFHFGKPGSGLKIYLQASLHAEEL
                     PGMLVAHHLRAHLEAAQAADQIQGEIVLVPVANPIGLAQRLDHKPMGRFELATSENFN
                     RHYPDLASAVAPAVMATLGADAAANVRLVRQAIGNYLQGWQPDTELQSQRRHLLWLAH
                     DADYMLDLHCDCEGVLHFYTEEPCWPQLADLAHFLGSQTILIAKNSGNLPIDECLSSA
                     WWQLAGRLKASGRNVPLPQGCCSSTIELRGELDVSHAWAKKDAQAIIDWLQFIGVLAC
                     DAKPLVPAPLCQATPLAGSQTLHARSPGIVVFAVEPGHILKKGDLVAEIIDPIANRTE
                     QVQAGVDGIFYARIRDRYITAGGELGKIAGAQAFRVGPLLGA"
     gene            complement(4422..5135)
                     /locus_tag="PLB25_13090"
     CDS             complement(4422..5135)
                     /locus_tag="PLB25_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517423.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE42550.1"
                     /translation="MEWAVIFEPVNLALFGNGILTTLWLLFASLAVGAVLALIFALML
                     TGPWASLRWLVGAYTFVIRGTPLLIQVYLIYYGLGQLEWIQARWNDVWPWTHFKEPFF
                     CALLAFSLNTAGYTAEMLAGAIRETSGGEIEAAHAYGMSRFQTMLRVVLPSAMRRTLP
                     AYSNEVVMMLHSTSLASAVPNMLDITSAANRIYATYYLPFEAYLAAAAIYLTASFCLI
                     GFFRFAEGRLLAYLAPQKH"
     gene            complement(5135..>5369)
                     /locus_tag="PLB25_13095"
     CDS             complement(5135..>5369)
                     /locus_tag="PLB25_13095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HOE42551.1"
                     /translation="PGFTNNWLVLIKATALVSLIGLQEMTYLAKQASAATRSPFAFFL
                     FTGALFLVYTSISLFVLKKVNARYSLGTKRGTL"
BASE COUNT         1127 a         1629 c         1609 g         1004 t
ORIGIN      
        1 agccaagcag cttggccgat ttgtcaaacg cctcgccggc cgcatcatca atggtttcgc
       61 ccattatctg gtagcgccct accccatcaa cctgcatcaa ttgcgtgtgc ccgcccgaca
      121 ccagcagcgc cacaaacgga aactcgggcg gatcagcgct caaaaacggc gacagcagat
      181 gtccttccag gtgatgcacg cccagcaccg gtttgcccaa cgcggccgcc agcgcgcagg
      241 cgacaccggc acccaccagc agcgcaccgg ccagaccggg gccgcgggta aacgccagca
      301 catcgacatc tgccagcgtg cggcccgatt cggccaacac ctgctcggtc aatggcagca
      361 cgcggcgaat gtggtcccgg ctggccagct cgggcaccac gccgccataa gcctggtgca
      421 tctcgatctg gctgtagagc gcgtgcgcca gcagcaccgg cactccaggg ccacgcacct
      481 cgaccagcgc cacgccggtt tcgtcacagg aagattcgat acccagaact tgcagcttgt
      541 ttgccatagc ggccaagttt aggcgatgca actgcaggca cttggtcgca ctggggaacg
      601 cttcgtgatc gccagacaac ggggccacca gtcggtcgtt caacgccagc ttcggccaat
      661 tgcccactgc aaaggcaact ttgaaaaatg ttcaagtgag cagcagaact ttcaccagaa
      721 agcaaacttg ctgatctagc tggtcttgcc tgagcatcgg tggtgaacag cgcgaatcag
      781 ggcctcgaat aattgggagg aacttcagtc cggatcagcg caaggcttct gagcttgatg
      841 acaaattggc ctccagccct tatgaatact gcgcgagaag ctctgcattt tgtagcataa
      901 tgaatgtacc agagtgaccg cgtcgatagc gagagccgac cccggcatgg caagcttgtg
      961 cggtacttgc cagcccacag cccagttgtg ggtcaaaagc caggcgccaa atgggtcgct
     1021 gcggtcgggt ttgcagccga tgcgctagca agtcgcgcag ccattcagga tagcttcaac
     1081 gggctggcgt agccggtcat ttccagataa ccacgcccta ttggctgccc cgactggttt
     1141 gacagttcgc ttaaaccttc ccagtaaatg gcaccagttg aactgcggct atcaagttcc
     1201 tgagcgtcca gcagggcgcg cacggtgaag ctggtgcgcg ctggcaaacc ggcatcaatg
     1261 gcagcggctg gcaggtcaat cgtccactgc accgggtaac tggcaccgct ggccgggctt
     1321 ttccagatgc gcccggcctt aaagcgaacg tcctgggccg caaacaccac cggcttggca
     1381 gcaccgccac cgcgccacga cccacccgcc cacaaggtgg cgccagcttt gttgcgcaag
     1441 cgaaaggcag tcagcgcagc gccatcgaac aaattcatgc caatccagtc ccagccaacc
     1501 gcctccgggt gcagcagcgc atcactccac tcgtgatcca gccaggcgcg tccgctgacc
     1561 cgccgggtgc tgccagccag ggtcaggtcg ccagcaagct tgagctgcgg caggctgtag
     1621 taaaaactgg cttgtgtggc ctgcggacct ttgcgcgacc aaccctgttc gccttgcagc
     1681 aacagcggct gggttggggt cagatcgagc gcaaaggcaa agtcctgcgc cgtcacccgc
     1741 gccagatagc ggccgtcttc gcgccgctgc agcgaccagt cgcgcagcct gatgtgggtg
     1801 tctgcctcgc tggcctccgc caggtccacc gatgccgtgc cggaactgcg ggcaatgcgc
     1861 tggtcatgca gcaagcgctg gccgctcagg tcagtgaccg cagcgtgcgc aaagatcagt
     1921 tgccgggcgg caaatgccga tgtcatgtgc tgcgtggcgg gcacgcgcga gcgaaaaaac
     1981 gtcaactgaa agccgtattg ctgctgcttg gcctggagtt caccggtgac gtaccaccac
     2041 tcgatggcgg tgtccgggtg gctgcccagg tcacgcgcaa acaccagctc acgcttgacc
     2101 agcgtggctg cggctgcacg gctgacttgc aaccaggcaa gccccggcca aagcggtgcc
     2161 agcagggcac gggtcagcag ttggcgacgc ggtagttgca aggcagacgg atttggcaat
     2221 ggcgtgcctg gtgctgacgg cagtgcgatt ggcctgacgg caatgcacca ttgaacggtc
     2281 ttctcatgat gctttcagcc tgtcggtgcg gtgcatcgag cgccgcaaat ttgaggtcag
     2341 aggcgtagtc ggatcgagat tgcgaacgga cttgcaagcc gggtccgcag ggggactcaa
     2401 cccaaaatct gctacctgtg tcaaatggac ggcgacgcag cagccacgtt gctgattcac
     2461 gggtcacttc agactgcccg acaaaaactg cgccagccgc acacttttcg ggttggacag
     2521 cacctgcgta gggttgcctt cttcttcaat ctggcccttg tgcaggaaaa tcaggtggtt
     2581 tgacacctcg cgggcaaaac ccatctcatg cgtcaccacc accatggtac ggccttccag
     2641 cgccaggttc ttcatgactt tgagcacctc ggacaccagt tcggggtcga gcgcgctggt
     2701 gggttcgtca aacagcatca cctcgggctc catcgccagc gcacgggcaa tcgccacacg
     2761 ctgctgctgg ccaccgctca agtgcgccgg gtaggcatct tccttgccct ccaggccgac
     2821 tttggccagg tacttgcggg cgattgcgat cgcctggtca cgcggcacct tcatcacatg
     2881 gaccggggct tcgatgatgt tttgcagcac cgtcatgtgc gcccacaggt tgaaatgctg
     2941 aaacaccatg gccagtttgg tgcgcaggcg ggccaattgc ttcggattgg cggcatgcaa
     3001 ttcgccgttc ggcgcggccg tcaggtgcaa ctcttcatca acaacatgta tccgccccag
     3061 gtgcggcttt tccagcaaat tgatgcatcg caaaaacgtg cttttacccg agccggaact
     3121 gccgatgatg ctgatcacat cacccgcatg ggccgtcagc gacacgccct tgagcacttc
     3181 gttggaacca aagcgcttgt ggatgttttc aacctgcagt ttgagaggag tcgtgtgggt
     3241 catggctttg gattgatggc gctgcgggcg gctttcaggc acccagcagc ggtccgacgc
     3301 gaaacgcctg ggcaccggca atcttgccca gctcgccacc agcggtgatg taacggtcgc
     3361 ggatgcgtgc gtaaaaaatg ccatccacac ccgcttgcac ctgttcggtg cggttggcaa
     3421 tggggtcgat gatctcggct accagatcgc cctttttcag gatgtgaccc ggctcgaccg
     3481 caaacaccac aatgccgggc gaacgggcgt gcagcgtctg cgagcctgcc agcggtgtcg
     3541 cctggcacag cggggccggc accagcggtt tggcatcgca ggccagcaca ccgatgaact
     3601 gcagccagtc gatgatggcc tgggcatctt ttttggccca ggcatggctg acatccaact
     3661 cgccgcgcag ctcaatcgtg ctgctgcagc agccttgtgg cagcggcaca ttgcgaccgc
     3721 tggccttgag tcgtccggca agttgccacc aggcgcttga aaggcattcg tcaatcggca
     3781 ggttgcccga gtttttggcg atcaggatgg tctggctgcc cagaaaatgc gccaggtcgg
     3841 ccagttgtgg ccagcaaggc tcttcggtat aaaaatgcag cacgccttcg cagtcgcaat
     3901 gcaaatccag catgtaatcg gcgtcgtgtg ccagccacag aaggtgacgg cgctggcttt
     3961 gcagttcagt atctggctgc cagccttgca ggtagttgcc gatggcctgg cgcaccagcc
     4021 gcacattggc agcggcatca gcgcccagtg tggccatcac ggcaggtgcc acggcgctgg
     4081 ccaggtcggg gtaatgccgg ttgaagtttt cagaggtagc aagttcaaag cggcccattg
     4141 gcttatggtc cagccgctgc gccaggccaa tcggatttgc cactggcact agcacgattt
     4201 cgccctgaat ctggtctgct gcctgcgccg cttccagatg agcccttaga tggtgcgcca
     4261 ccagcatgcc cggcagttct tcggcatgca ggctggcctg caggtaaatt ttcagacccg
     4321 agccaggctt gccaaaatga aagctggtga tggttttctg gctgcccaga ctgttgctga
     4381 gcaaggggtg atcaaggcgc tgcatgtgtc tggttaccaa ttcagtgttt ttgcggtgcc
     4441 agataggcca gcaaacggcc ttcggcgaag cggaaaaaac cgatcaggca aaacgaggcc
     4501 gtcaagtaaa tggcggcagc ggccagatag gcctcaaacg gcaggtaata ggtggcgtaa
     4561 atgcggttgg cagcggaggt gatatcgagc atattgggca cagcgctggc caggctggtg
     4621 ctgtgcagca tcatcaccac ctcattgcta taggccggca gggtgcggcg catggcactc
     4681 ggcagcacca cccgcagcat ggtctgaaag cggctcatgc cgtaggcgtg ggctgcttca
     4741 atttcgccgc cgctggtttc acgaatggca ccggccagca tttcagcggt gtaaccggca
     4801 gtgttcaggc tgaatgccag caacgcgcaa aaaaacggtt ccttgaagtg ggtccagggc
     4861 cagacatcgt tccagcgcgc ctgaatccat tcaagctgac ccaggccgta ataaatcaga
     4921 tagacctgaa tcagcaaagg tgtgccgcga atcacaaagg tataagcacc caccagccag
     4981 cgcagcgagg cccatgggcc ggtcagcatc agcgcaaaaa tcagcgccag cacggcaccg
     5041 accgccagcg aagcaaacag cagccacagt gtggtcagga tgccgttgcc aaacagggcc
     5101 aggttgactg gctcgaaaat gacggcccat tccattacaa ggtgcctcgt ttggtgccca
     5161 ggctatagcg cgcattgacc tttttgagga caaacaagga aattgacgtg taaaccagaa
     5221 acagcgctcc ggtaaacaag aagaaagcaa agggggaccg ggtggcagca ctggcctgct
     5281 tggccagata ggtcatttcc tgcaggccaa tcaagctcac cagcgcggta gccttgatca
     5341 aaaccagcca gttgttggtg aaaccgggc
//
LOCUS       DAOSLL010000301         5366 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13673_length_5366_cov_4.669554, whole genome shotgun sequence.
ACCESSION   DAOSLL010000301 DAOSLL010000000
VERSION     DAOSLL010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13673_length_5366_cov_4.669554"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1689)
                     /locus_tag="PLB25_13100"
     CDS             complement(<1..1689)
                     /locus_tag="PLB25_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE42552.1"
                     /translation="MKTPTLERMSLPLSPEKSSPNRLSPRARLRLWWSQQSPHRQDRF
                     AMLAPLAAVVLFLLAIMVAFAYLRFEEIAREREAVNRDIEYTQQRLRLRLLEQQEQFM
                     RFAREITSREIGNTGFMARAEAFANIYPELQGLTWIDDKKKVRVSYNTASRSDQYQNS
                     TGSSIEAKDTLINFQAARDLLLPIYSTPRAQPNLSAVLQLHLPLIDKARFKGDLLVEY
                     SIDALYRYGLPMEITSKYAVSFHDIDGRALAGISVIEGNLKRHFLPWANPINAYETPV
                     SPVGNGLTIRGQVYRTSLGLIGSGLFWLVMVLSAVTAWLLWANWRHTRRRIQAQDALM
                     RETAFRRAMENSMLTGMRAMDLHGRITYVNSAFCSITGWSESDLVGRTAPFPYWPEED
                     IEILKARLAEELAGRVAPSGIQFRVKRKDGNFIDAHIYVSPLIDAFGQQTGWMTSMSD
                     ITEPNRVREQLAASHERFTTVLEALDASISVAPLGNDELLFANKLYRLWFGTHAQGHL
                     QLAAEAGVPALAHSEETQDAVDAFAGLPTNHLHDKEAESAEVYLGTLGRWLEVRT"
     gene            1832..4552
                     /gene="aceE"
                     /locus_tag="PLB25_13105"
     CDS             1832..4552
                     /gene="aceE"
                     /locus_tag="PLB25_13105"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HOE42553.1"
                     /translation="MSAALSNPSGTSAIDIDSLETREWTDALSAVIQSEGPERAHFLL
                     EQLLEHARQNSIDMPFSANTGYVNTIETDQEERCPGNIEIEERLRAYMRWNAMAMVVK
                     ANRHHPEDGGDLGGHIGSFASLAHMFGAGFNHFWHAESADHGGDCLYIQGHVSPGVYA
                     RAYLEGRLSEEQLLNFRQEVDGKGLSSYPHPKLMPTFWQFPTVSMGLGPLMAIYQARF
                     LKYLHARGIANTENRKVWVFCGDGEMDEVESLGAIGLAAREKLDNLIFVVNCNLQRLD
                     GPVRGNGKIVQELEGEFRGSGWNVIKLLWGSNWDPLLARDKNGALRKIMMDTLDGDYQ
                     SFKANDGAYVRKNFFGRSPETLEMVAKMSDDDIWALRRGGHDPQKVYAAYHKAVNHKG
                     QPSVLLIKTVKGFGMGKSGEGKNNVHQTKKLTDEDIKAFRDRFNIPVPDSQLASIPFY
                     KPAEDTPEMRYLHERRQALGGYLPSRRAKADEQFTVPSLDIFKSVMDPTAPGREISTT
                     QAYVRFLTQLLRDQALGPRVVPILVDEARTFGMEGLFRQIGIYNPAGQQYTPVDKDQV
                     MYYKEDVKGQVLQEGINEAGGMASWIAAATSYSTSNRIMVPFYVYYSMFGFQRIGDLA
                     WAAGDMQARGFLLGGTSGRTTLNGEGLQHEDGHSHILAGTIPNCVSYDPTFAHEVGVI
                     LHHGLQRMVERQENVFYYLTLLNENYAMPGLTAGTQEQIIKGMYLCKPGAEGLPGAQA
                     ARVQLLGSGSILRESLAAQQLLAVDWGVAADVWSCPSFNELARDGQACDRHNLLHPGA
                     APLLSFVATQLGNSSGPVVDSTDYIKAYAEQIRPYIPKGRCYKVLGTDGFGRSDFRSK
                     LREHFEINRHYIVLAALKALSEDGTVPVARVAEAIAKYGIAADKVNPLYA"
     gene            4696..>5366
                     /locus_tag="PLB25_13110"
     CDS             4696..>5366
                     /locus_tag="PLB25_13110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HOE42554.1"
                     /translation="MAILEIKVPDIGDFAEVVVIELLVKAGDSVKAEQSLLTVESDKA
                     SMEIPSSHAGVVKELKVQLGDKVSEGSLVLLLDVAEQAVQSVAAAAPASSPAAADLVS
                     NQAVALADQALVAPVIEAVGSQSVELRVPDIGDFKDVTVIEVMVKQGDSIRLEQSLIT
                     VESDKASMEIPSSSSGVIKSLLVKLGDKLNIGDLLAIVEAPAAPASAAPASSPAASPN
                     SESKQA"
BASE COUNT         1149 a         1562 c         1587 g         1068 t
ORIGIN      
        1 ggtgcgcacc tccagccagc gccccagcgt gcccagatag acctcggcgc tttcggcttc
       61 tttgtcatgc aagtggttgg tgggcaaccc ggcaaatgcg tccactgcat cttgtgtttc
      121 ctcactgtgg gccagcgcgg gtacaccggc ttcagcggca agctgcaagt ggccttgtgc
      181 atgggtgccg aaccacagcc ggtatagctt gttggcaaac agcagttcgt cattgcccag
      241 cggggccacc gagattgatg cgtccagcgc ttcgagcacc gtggtgaagc gctcgtgcga
      301 tgcagccagt tgctcgcgaa cccggttcgg ctcggtgatg tcgctcattg atgtcatcca
      361 cccggtttgc tggccaaagg catcaatcaa cggcgagacg taaatgtggg catcaatgaa
      421 gttcccatcc ttgcgcttga cccgaaactg gatgccgctg ggcgcaaccc gcccggcaag
      481 ctcttcggcc aggcgtgctt tcagaatctc gatgtcttcc tcgggccaat acggaaacgg
      541 cgctgttcgg ccaaccagat cactttcgct ccagccggtg atgctgcaaa aggccgaatt
      601 gacataggtg atgcggccat gcaaatccat ggcacgcatg cctgtgagca tcgagttttc
      661 catggcgcgg cgaaaagccg tttcgcgcat cagcgcatcc tgcgcctgaa tccgcctgcg
      721 ggtgtgacgc cagttggccc acagcagcca ggccgtcacc gcactgagta ccatcaccag
      781 ccaaaacaga ccgctgccga tgagccccag cgaggtgcga taaacctgcc cgcgaatggt
      841 caggccgttg ccgaccgggg agaccggtgt ctcgtaggca ttgatcgggt tggcccaggg
      901 caggaaatgt cgcttgagat tgccctcgat gacgctgatg ccagccagtg cgcgtccgtc
      961 aatgtcgtga aaggacacgg cgtacttcga ggtgatctcc atcggcaagc cgtagcggta
     1021 caaggcatcg atcgaatatt ccaccagcag atcgcccttg aagcgggcct tgtcgatcag
     1081 cggcaggtgc aactgcaaga cggcagacag gttgggctgg gcgcgtggcg tggagtaaat
     1141 cggcaaaagc aggtcgcgcg ctgcctgaaa gttgatcagc gtgtctttgg cttcaatgct
     1201 ggagccggtg ctgttttggt attggtcgga gcgactcgcg gtgttgtagc tgacccgtac
     1261 cttcttttta tcgtcgatcc aggtcagtcc ttgtagttcc gggtaaatgt tggcaaaggc
     1321 ctcggcgcgt gccatgaaac cggtgttgcc gatttcacgg ctggtgattt cgcgcgcaaa
     1381 ccgcatgaac tgttcctgct gctccagcag ccgaaggcgc agtcgctgct gggtgtattc
     1441 aatatcgcgg ttgacggctt cgcgctcgcg cgcaatctcc tcaaaacgca aataggcaaa
     1501 ggccaccatg atcgccagca gaaacagcac cacggcagcc aacggtgcca gcatggcaaa
     1561 acggtcctgc cggtgcgggc tttgctgcga ccaccagagc cgcaggcgcg ctcggggcga
     1621 gagtcgatta ggactcgatt tttccgggct gagcggcaag gacatgcgct caagtgtagg
     1681 agttttcaag ctgaaattgg cgtgtgcaat atttcataat atggaatcaa tatacgctat
     1741 ttgaaattct gttataaatg ggcataaaat caatccatca caagcaagac tatctccatt
     1801 aacatcactc ggatacaacc aggaatcaac catgtctgca gcattgagca acccctctgg
     1861 caccagcgcg attgacatcg acagcttgga aacccgtgaa tggactgacg cattgagcgc
     1921 ggtcattcaa agcgaaggcc ctgagcgcgc ccacttttta cttgagcaat tgcttgagca
     1981 cgcgcgccag aacagcatcg acatgccgtt ttcagccaat accggttacg tcaacacgat
     2041 cgagaccgat caagaagagc gttgcccagg caacatcgaa atcgaagaac ggctgcgcgc
     2101 ctacatgcga tggaacgcca tggccatggt ggtcaaggcc aaccgccatc accccgaaga
     2161 cggcggtgac ctgggcggtc acattggctc atttgcctcg ctggcgcaca tgtttggtgc
     2221 tggcttcaac catttctggc atgccgaaag cgcagaccat ggtggcgact gcctgtatat
     2281 ccagggccat gtgtcccctg gtgtctatgc ccgggcctat ctggaaggcc gcctttctga
     2341 ggagcagttg ctcaacttcc gccaggaagt cgatggcaaa ggcctgtcaa gttacccgca
     2401 ccccaagctg atgcccacgt tctggcaatt tcccaccgtg tcgatggggc tggggccgct
     2461 gatggcaatt taccaggcgc gatttttgaa atacctgcac gcgcgtggca ttgccaacac
     2521 cgaaaaccgc aaggtctggg ttttttgcgg tgatggtgag atggacgagg tcgaaagcct
     2581 gggtgcaatt ggcctggccg cgcgtgaaaa gcttgacaac ctgatctttg tcgtcaactg
     2641 caacctgcag cgcctggacg gcccggtgcg tggcaatggc aagatcgtgc aggagcttga
     2701 aggcgagttc cgaggctcgg gctggaacgt catcaagctg ctgtggggta gcaactggga
     2761 cccgctgctg gcgcgcgaca aaaacggcgc gctgcgcaag atcatgatgg atacgctcga
     2821 tggcgactac cagtcattca aggccaacga tggtgcctac gtgcgcaaga atttctttgg
     2881 tcgcagcccc gagacgctgg agatggtcgc caagatgagc gatgacgaca tctgggcctt
     2941 gcgtcgtggc ggtcatgacc cgcaaaaggt ctatgcggcc tatcacaagg cggtcaatca
     3001 caagggtcag ccgtcggtgc tgctgatcaa gaccgtcaag ggctttggca tgggcaagtc
     3061 aggcgagggc aaaaacaacg tgcaccagac caaaaagctc accgacgaag acatcaaggc
     3121 ttttcgcgac cgcttcaaca tcccggtgcc cgacagccag ttggccagca tcccgtttta
     3181 caagccggct gaagacaccc ccgaaatgcg ctacctgcat gaacgccgcc aggcgcttgg
     3241 cggctacctg ccgagccgtc gtgccaaagc cgacgagcag ttcacggtgc cctcgcttga
     3301 tatcttcaag agtgtcatgg atccaaccgc acctgggcgc gagatttcca ccacccaggc
     3361 ttatgtgcgc tttctgaccc agttgctgcg cgaccaggcg ctcggcccca gagtggtgcc
     3421 gattctggtc gatgaagccc gcacctttgg tatggaaggc ctgtttcgcc agatcggcat
     3481 ttacaacccg gcaggccagc agtacacccc ggtcgacaaa gaccaggtca tgtactacaa
     3541 ggaagacgtc aagggccagg ttttgcagga aggcatcaac gaagccggcg gcatggcaag
     3601 ctggatagcg gctgccacca gctacagcac cagcaaccgc atcatggtgc cgttttatgt
     3661 ctattactcg atgtttggct ttcagcgcat tggcgacctg gcctgggctg cgggcgacat
     3721 gcaggcgcgc ggctttttgc tcggcggcac cagcgggcgc accacgctca acggcgaagg
     3781 cctgcagcac gaagacggcc acagccacat tctggctggc accattccca actgcgtcag
     3841 ctatgacccg acctttgccc atgaagtcgg ggtgatcctg caccacggcc tgcagcgcat
     3901 ggtcgagcgc caggagaatg tcttttatta cctgaccctg ctcaatgaaa actatgccat
     3961 gccgggtctg acggctggca cgcaagagca aatcatcaaa ggcatgtacc tgtgcaagcc
     4021 gggtgccgaa ggtttgccag gtgcgcaagc ggcgcgcgtg cagttgctcg gatcgggcag
     4081 catcttgcgc gagagcctgg ccgcgcaaca actgctggcc gtcgattggg gcgtggcggc
     4141 cgatgtctgg agctgcccca gcttcaacga gctggcacgc gacgggcagg cctgcgaccg
     4201 ccataacctg ctgcacccag gcgctgcgcc attgctgtca tttgttgcca cgcagcttgg
     4261 caactcttcg ggcccggtgg ttgactcgac cgactacatc aaggcctacg ccgagcagat
     4321 tcgcccgtac attccaaaag gccgctgcta caaggtgctc ggcaccgacg gttttggccg
     4381 cagtgacttc cgcagcaaac tgcgtgagca cttcgagatc aaccgccact acattgtgct
     4441 ggccgcactc aaggcactca gcgaagacgg tactgtgccg gttgccaggg tggccgaggc
     4501 gattgccaaa tacggcattg cagccgacaa ggtgaatcca ctttacgctt aaccatttcg
     4561 tcacaggcaa cgcgcagccg ggtcttttca cccgccaggt tggccggatg tgatccggca
     4621 actttgaatg gcggctcaga cccgccctga cagtcgcgtt tgctgcggga cgatcaacat
     4681 atctggagac acatcatggc aattctggaa atcaaggtcc ccgacattgg tgacttcgcc
     4741 gaagtggtcg tcatcgaact gctggtcaag gccggcgaca gcgtcaaggc cgaacagtcc
     4801 ctgctaactg tcgaatccga caaggcatcg atggaaatcc cgtcaagcca cgccggtgtg
     4861 gtcaaagagc tcaaggtgca gcttggcgac aaggtcagtg aaggctccct ggtgctgctg
     4921 ctcgacgtag cagagcaagc tgttcaaagc gtcgcagcgg ccgcaccagc aagctcaccg
     4981 gctgccgcag atttggtatc aaatcaggct gtagcccttg ctgatcaagc gctggtagct
     5041 cctgttattg aagcagttgg gagccagtcg gtggagctgc gggtgcctga cattggcgac
     5101 ttcaaggatg tcacggtgat cgaggtgatg gtcaagcagg gcgacagcat ccggcttgag
     5161 caaagcctga tcacggtcga gtctgacaag gcttccatgg aaatcccgtc aagcagttcg
     5221 ggtgtcatca agtcgctgct ggtcaagctt ggcgacaagc tcaatattgg cgatttgctg
     5281 gccattgtgg aagcccccgc agcgcctgcc agcgcagcgc ctgccagcag tccggctgcg
     5341 tcgccaaatt ctgaatcaaa acaggc
//
LOCUS       DAOSLL010000302         5361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13690_length_5361_cov_3.246702, whole genome shotgun sequence.
ACCESSION   DAOSLL010000302 DAOSLL010000000
VERSION     DAOSLL010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5361
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13690_length_5361_cov_3.246702"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            385..2817
                     /locus_tag="PLB25_13115"
     CDS             385..2817
                     /locus_tag="PLB25_13115"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE42555.1"
                     /translation="MIPHIIHQTWKTSEVPQRFVYLRDTWLKHHPGWTYRLWSDADNR
                     AFLAEHYPAFLPVYDAYQTPICRADAIRCFLLKHFGGLYVDLDFECFAPLDGILNSAS
                     VILGLEPPAHVRDHKPLLKGLERVVCNALMASPAQHPFWDHMISEMAKARSEPDPLDA
                     TGPFLLTRAVDSYQGTARVEVTDTGVFYPADVYDCNQGRLFDLEYWHRVTQGALAMHH
                     WAGTWWRGSLEVPPLPSEVVGLAVVESGKILIQATLHARHCTPLGQSAPLVSCLMVTR
                     NRSALAICAVKAFLAQSYPPRELLILDDGDDRQLERFLAALADPRIRYLHLPDTGATL
                     GTLRNQALALARGSYVCQWDDDDLYDPGRLAMQMTAMAAMQADACFLHRWVIWWPKQN
                     RLAVSSQRVWEGSMLCLKSKVGQYPTLRRGEDTPVAERIMQSERVVLLDQPRLYVYVV
                     HEGNTFESAHFESQWQSASLKFAGEGYARVMQEVGKGLDIESYLLALDQKAAKTSPEP
                     RSSLAAPQLAAQATAADANGPKSSQTNPQPTSSAAPAALRRPVRQTATPSVLILTPIK
                     NAAQFLPGFFELATRLDYPPDKLSLAFLEGDSDDGTAALLQDYALRYRNQFAAADVYQ
                     HDFSYQMEGERWAAEKQFSRRSVISKCRNMLFKLADRGQERILWIDADVINYPPDILL
                     QLLEPGLSIVVPNCVQVAGGPTFDLNTFKFRPGSQNSEWRYLLDGIIQPPRGASRLYL
                     DELRGQPMVELDGVGGTMLLVDAKLHRQGILFPDYSYRGYLDTEGFAMHAKDRGISAW
                     GLPDLEIIHASQ"
     gene            2911..3555
                     /locus_tag="PLB25_13120"
     CDS             2911..3555
                     /locus_tag="PLB25_13120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42556.1"
                     /translation="MSPTHNILIGTPAYAGMVHLDYLNSISEYYRSQINFTISAIGNE
                     SLITRARNAILSKFFEEPEFTHLLFLDGDVYLPASGLQRLLSHDVDAVGAAVPLKGFN
                     ERGERIFNVGQCLGEQGPLHEVERIGTAALMLSRNAVNDLVAEAEQAGDTYQRLLSRG
                     TPMARVHYDVFKVGVVNGDYLSEDFWVCHRLRHMGYRIFVDPEIATRHQGTTQF"
     gene            complement(3593..4141)
                     /locus_tag="PLB25_13125"
     CDS             complement(3593..4141)
                     /locus_tag="PLB25_13125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42557.1"
                     /translation="MNSEPSEQSQKSVSARRRLFQILAVGGTAAVVLPTKWVKPVVDA
                     VIVPAHAAGSVVRVSGIYGNSGGVLLGSASVLDRFTGMLMSSAQANNIQSCANTVCIS
                     FDIPANGNTVQIWALNVPGTTNIQANNTLDNVTVGQLAFSGMYADSVYLRGTITATPN
                     ALCSSGTFTFPRVSAACNSAPT"
     gene            4255..4614
                     /locus_tag="PLB25_13130"
     CDS             4255..4614
                     /locus_tag="PLB25_13130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42558.1"
                     /translation="MNFSVISPPMSSNMLQALLGFHLRGNNACCHDSLRKCHEFAQTP
                     LGLSGLIKTTGAVNLRSDPNFQPPISNEGSGHTGNWALTPKSRASITLYMLNQLLDPV
                     EYALAAIVFDKFGSANP"
     gene            complement(4986..>5361)
                     /locus_tag="PLB25_13135"
     CDS             complement(4986..>5361)
                     /locus_tag="PLB25_13135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42559.1"
                     /translation="NSLQGVSSGRILERFAGMIIGSAHAQLNPYICGSLTNTVCISFS
                     IEPSPSTSVAVQVSGVSSAPTVIYPNNVIADVEVGGGLFRFTNLIASSVSVSGFVTSS
                     GVPNCVPEAFSFPPATVCFSGD"
BASE COUNT         1197 a         1549 c         1500 g         1115 t
ORIGIN      
        1 agtaaagcag cggaccaact tgctggcgtg ccgccgcttt aggtacggct gaccaatcgc
       61 gaacctgcct ggtctgcgca agtaacaggt cggcacgcct ctggctaagt gttggtgagc
      121 aaatcaggcc aaacaggctg taacaatgag cggacatgca gaggtttggc agaatagatt
      181 tgaaacaaaa gagttggctg cgctggcacg gcagtcgagc tgcgaggata gccgaaaata
      241 ctgattcaag gcatcagacc agcgggcgga caagccagtc tggcgaccga tggctaaccc
      301 tggcggcgtg tcagaatccg gggggccatc acccctgcgg cttcgggccg tcaattgccc
      361 ctcaacagtg ccggagcctc acccatgatt cctcacatca tccaccagac ctggaaaacc
      421 agcgaggttc ctcaacgttt tgtctacctt cgtgacacct ggctgaaaca ccatcccggc
      481 tggacctatc ggctatggag tgatgccgac aaccgggcat ttctggccga acattacccg
      541 gcgtttctgc ccgtgtacga tgcttatcag acacccatct gccgggccga cgcgatacgc
      601 tgttttctgc tgaaacactt cggcggactc tatgtcgatc tggattttga gtgctttgcg
      661 cctcttgatg gcatcctgaa ttctgccagc gtgatccttg gcctggaacc accagcccat
      721 gtccgtgacc ataagccgct gctcaagggc cttgagagag tggtttgcaa tgccttgatg
      781 gcatcgccag cgcagcaccc attttgggat cacatgatca gtgaaatggc aaaggcccgc
      841 agcgagcccg acccgctgga tgccaccgga ccctttttgc tgacccgggc ggtggacagt
      901 taccagggca cggccagggt ggaggtcact gatacaggtg ttttttaccc ggccgatgtg
      961 tatgactgca accagggacg gctgtttgat ctggaatatt ggcatcgggt tacccaggga
     1021 gcgctggcca tgcaccattg ggctggcacc tggtggcgtg gatcgcttga ggtgccaccg
     1081 ctgcccagcg aggtagtcgg cctggccgtg gttgaatctg gcaagatcct gatccaggcc
     1141 acgctgcatg ccaggcactg cacaccgctg ggccaatcgg ccccgctggt gtcttgcctg
     1201 atggtgaccc gcaaccggtc agccttggcc atttgcgccg tcaaggcctt tctggcgcaa
     1261 agctacccgc cgcgtgagct gctgatcctc gacgacggcg atgaccggca gcttgagcga
     1321 tttctggcgg ccctggccga cccgcgcatt cgttacctgc acctgcccga caccggcgcc
     1381 acgctgggaa ccttgcgcaa ccaggcgcta gcgctggcac gcggcagcta cgtctgccaa
     1441 tgggacgatg atgatctgta tgacccaggt cggctcgcca tgcagatgac ggcgatggcg
     1501 gccatgcagg ccgatgcctg ctttctgcac cgctgggtga tctggtggcc caaacaaaac
     1561 cggctggccg tgtcaagcca gcgcgtgtgg gaggggtcaa tgctgtgcct caagtcaaaa
     1621 gttggccagt atccgaccct gcgccgaggc gaagacacac cggttgcaga gcgaatcatg
     1681 cagtctgaac gggtcgtctt gctcgatcag ccccggctct atgtgtatgt ggtgcacgaa
     1741 ggcaacacct ttgagtcagc gcattttgag tcgcagtggc aatctgccag cttgaagttt
     1801 gctggcgaag ggtatgcccg tgtgatgcag gaggtcggca aggggctgga cattgaaagt
     1861 tacctgttgg cgctagacca aaaggccgcg aagacatcac cagaaccccg atcaagcctc
     1921 gccgcacccc aattggccgc ccaagcgacc gcagccgatg ccaatggccc gaaaagcagc
     1981 cagaccaacc cgcagccgac gtcttcagcc gcaccggctg ccttgcgtcg gccggttcgc
     2041 caaacagcaa cgccgtccgt tctgatcctg accccgatca aaaatgccgc ccagtttttg
     2101 ccgggctttt ttgagttggc gaccaggctt gactatccac ctgacaaact cagcctggcc
     2161 tttctggagg gcgacagcga tgacggtacg gctgcgctgt tgcaggacta cgcgctacgc
     2221 tatcgcaacc aatttgccgc agccgacgtt taccagcacg atttctcata tcaaatggag
     2281 ggcgagcgct gggctgccga gaaacagttt tcgcgcagaa gcgttatctc gaagtgtcgc
     2341 aacatgctgt tcaaactggc agaccgtgga caagagcgca ttctctggat cgatgcggat
     2401 gtcatcaact atccgccaga cattctgctg caactgcttg aaccaggctt gtcaattgtg
     2461 gtgcccaact gcgtgcaggt cgccggtggc ccgacttttg acctcaacac cttcaaattc
     2521 aggcccggca gccaaaattc ggaatggcgc tacctgctcg acggcatcat tcagccgcca
     2581 cgcggcgcga gccggcttta ccttgacgaa ctacgtggac aaccgatggt tgaactcgac
     2641 ggggtcggcg gcaccatgct gctggtggat gcaaaactgc accggcaagg cattttgttc
     2701 ccggactaca gctaccgtgg ctatctggac accgagggct ttgccatgca cgccaaagac
     2761 cggggaattt ctgcctgggg gctgccagat ctggaaatca tccacgcatc acaataacag
     2821 agcgcctgac tttggctttg ccgggcggct ggcagcatcc ccgcgtttct ggcgaagtct
     2881 cttgaatcaa cttgaacgga aaaagacgct atgtcaccaa cacacaatat tctgattggc
     2941 accccagcct acgccggcat ggttcatctg gactacctca attcgatttc agagtattac
     3001 cgctctcaaa tcaatttcac catatcggcc attggcaatg agagcctgat cacgcgcgcg
     3061 cgcaacgcca ttttgtcgaa gtttttcgaa gagcctgaat tcacccatct gctgtttctt
     3121 gacggcgacg tttacctgcc agccagcggc ttgcagcgct tgctctcgca tgatgtggat
     3181 gccgttgggg cggccgtgcc actgaagggc ttcaacgaac ggggcgagcg cattttcaat
     3241 gtcggtcagt gtctgggcga acaaggcccc ttgcatgagg tggaacgtat cggtaccgct
     3301 gcgctgatgc tgtcgcgaaa tgctgtcaat gacctggtgg cagaggccga acaagccggc
     3361 gacacctacc agcgcctgct ctccagaggc accccgatgg cgcgggtgca ctacgacgtg
     3421 ttcaaggtgg gggtggtcaa cggtgactac ctgtcagaag acttctgggt ttgccaccgt
     3481 ttgcgccaca tgggataccg aatctttgtt gaccctgaaa ttgccacccg tcaccagggc
     3541 acgacacagt tctagcggca tctgctgcgg cccgatggca gcgaagccca aatcaggtcg
     3601 gtgcgctatt gcaagcagcc gaaactctcg ggaaggtgaa cgttccggaa ctgcacaggg
     3661 cgttcggcgt tgcggtaatg gtgccacgca aataaacaga atcggcgtac atgccactga
     3721 acgccaactg accaacagtg acattgtcga gggtattgtt cgcctgaatg tttgtggtgc
     3781 cgggaacgtt gagtgcccat atctgaacag tgttgccatt ggcaggaata tcaaatgaaa
     3841 tgcagaccgt attcgcgcaa gactggatat tgttcgcctg agccgaactc atcagcatgc
     3901 cagtgaaccg atccagaaca ctggcactgc ctaagagcac tccgcctgag ttgccgtaaa
     3961 tgcctgatac ccgcaccaca ctgcccgccg catgcgccgg cacgatcacg gcatcgacca
     4021 ccggcttgac ccatttggtc ggcaaaacaa cggcggcagt accacccaca gccaaaatct
     4081 ggaacaggcg acgtcttgcc gataccgatt tttgtgattg ttcagaaggt tcggagttca
     4141 tgtgattcct ttgctgttaa aggttaatta gtgtttccgt agatgataca gcagcctaaa
     4201 ctgcccaact gcggctgttc catcgcctgt aacagcctgt gaagaaactg gaagatgaat
     4261 ttttcagtca tttctccgcc tatgtcctcc aacatgttgc aagcgctgct aggtttccat
     4321 ctgcgcggca acaacgcgtg ttgccatgac tcactgcgca agtgtcatga gtttgcgcag
     4381 accccactag gcttgagcgg gttgataaag accactggtg cagtgaattt gcggtcggat
     4441 cccaatttcc agccacccat ttcaaacgag gggtccggcc acacagggaa ttgggctctg
     4501 accccaaaat cacgggcatc gatcacgctt tatatgctaa atcaactgct agaccccgtt
     4561 gaatatgcgc tggcagctat tgtttttgat aagttcggca gcgccaatcc gtgatatggt
     4621 ggtctgagcc cactggctga acagggtgac cacctgtcca cccctacggt cagccattgg
     4681 gatccgaccc caaaatctct gcaccggtta cgcctcaccg tgcaggaagt gaacgcgaac
     4741 atttcaggac gaaaagccct ggcttggcca tgggcaaggg gcggctggat gccgcaggtg
     4801 tcgcgagttt gaacaggccc ttcattggcg atcatgacaa ggttgtcaaa ctcgccagtg
     4861 cgacgcacac caaacaactc gttaggccga gtgcatagca aggtgcttca aggcgcgggc
     4921 attgaacagt ggcaatttgc cagaaggctt ttgggcctgc cgccgctcac accccatcag
     4981 ctacgtcagt cgccgctgaa gcagacagtc gcaggaggaa aggaaaacgc ctcagggacg
     5041 caattcggaa ccccgcttga cgtaacaaaa ccagagactg aaacagaact ggcaatgagg
     5101 ttagtaaatc gaaagagccc accccctact tcgacatcgg ctatcacatt gttgggatag
     5161 attactgtgg gggcagaact cacgccagaa acctgcacag ccactgatgt gctcggagag
     5221 ggctcaattg aaaatgaaat acagacggta ttggtcaacg aaccacaaat ataagggttg
     5281 agttgcgcat gcgctgaccc aatgatcatc ccggcgaagc gctccagtat gcggccactg
     5341 ctaacacctt gcaaactatt a
//
LOCUS       DAOSLL010000303         5354 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13711_length_5354_cov_4.053595, whole genome shotgun sequence.
ACCESSION   DAOSLL010000303 DAOSLL010000000
VERSION     DAOSLL010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5354)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5354
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13711_length_5354_cov_4.053595"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1653
                     /locus_tag="PLB25_13140"
     CDS             <1..1653
                     /locus_tag="PLB25_13140"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE42560.1"
                     /translation="LDQSREILLLVDPVTLAICEVSKPGLRQLGYTLEELLGRPITDI
                     ECALSDLFFWDEVRQGGNPNITDTECSYLCSNGEILLASKTVLRVESDGTSWLVIRAE
                     PLEPRQQAESELASISSRLQATLEATAEGILLLDREGRILNMNQCFSRMWGIPDALLA
                     RRVDEEAFAFMASFFADQEAYQRNLAAIHLDSDLESFDTLELTDGRIFERRSRPARYA
                     EQIIGRVFSFSDATQRKAIEQQLLNQNARLEEVVQQRTAALSQQLAALTESNRKLEEA
                     QFQLLQSEKLASIGQLAAGVAHEINNPIGFINSNLGTLGDYVESLLAIDAAYNQVQHA
                     LDPSVQLAFEPVNALKISLDYAFMVDDLRQLIKESRDGLGRVKTIVRDLKDFARVGSA
                     EWAWSDIHQGLESTLNIVWNELKYKATVERDYATLPRVHCIPSQLNQVFMNLLVNAAH
                     AIEGQGVIRVRTGCNDKQVWVEVIDTGIGIPPKNLNRIFDPFFTTKALGKGTGLGLSL
                     AWGIVQRHRGSIEVQSEPGKGTTFKVLLPIGGEPAEAQPSSQ"
     gene            1949..3253
                     /locus_tag="PLB25_13145"
     CDS             1949..3253
                     /locus_tag="PLB25_13145"
                     /inference="COORDINATES: protein motif:HMM:NF013306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HOE42561.1"
                     /translation="MQASLFGQQLTLMEELKAETFGSHARLQTAPFFQALAGCHLPLE
                     SYVGQLRALLAIHAVVEQAVIDCVDDRVGSVFSEDMCKLGLLRKDLQYFEPRTVADLK
                     VAVDVAQQTTSALREQSMEAPLAVLGWLYVLEGSTLGTTVLQPLVARAFLLVGDDGMA
                     YLRNYGAAARSRWAEYSQRMNTLALETGERAQIAQAANELFSRLEALFRALYPFQHES
                     KAYLAASINPEAGRHPVPDDVREVHASVRAGDICWHRFPYFAARYGERGLGFARSDAA
                     WQATLYPYPPAQLLQQVQWLGRLLSARGMPTLLLQVQLEILVEELATAIPERRADYEN
                     LLPAAAALHAARSRFITDAQAQELAAAFDLAVGPEWRARFPHSGSLLTAAVADECGGC
                     EKAVESLRTWMTDVRQFPTAWIDAVESTLAQARQQAAQKAKT"
     gene            3250..3900
                     /locus_tag="PLB25_13150"
     CDS             3250..3900
                     /locus_tag="PLB25_13150"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOE42562.1"
                     /translation="MNTAIDEATYAHYLAALIAGDRETCARTVQALLHAGTGLKPLYV
                     GLLQRAQYQVGELWEQEKISVAVEHLSTAITQRMLSLIQAHTLQGARRNRSIVVACVA
                     DEFHQLGGRMVADFCELRGWRGYFLGANTSVEDLLKLISQRKPHLLGLSLSIYFNLPK
                     LIAALDAVTAAYPDLPILVGGQAFRWGAQDALQHYPHAAYMESLDALEERLAACER"
     gene            3890..5143
                     /locus_tag="PLB25_13155"
     CDS             3890..5143
                     /locus_tag="PLB25_13155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE42563.1"
                     /translation="MSADATLYPEGSGRHFLAASASVVVARVNASGTLLATNQHAVNL
                     IGEPLVGKPWHTMLLNFGGPPVLQEWLAKPARPRLLNIKTAAGLPQTLEVTVEPDGAD
                     YLLFGEVNATEQARLSREVLQLNHELNNMSRELALKNDDLVKTQSQLVQSEKLASIGL
                     LAGGVAHEINNPLAVVKSNLGQLRQYVKTLLTVIRASDAIEADASGSSSALAALQSLK
                     QESDLPFIKNDVVFLLAECDESVDRVEKIVRGLRDFSRMDVRETLAFEDIHQALESTL
                     TTIGPELTTRCEIRKEYGDIVPVQCMRGEICQVFMSLLVNAMKAMKDPGVITICTGQE
                     KSEARIEISDTGHGIAQTDLPLIFDPFFTTRPVGQGTGLGLAVAYAIVQRHHGRIEVA
                     SALGAGTTFRVWLPIVQPVEVAPTQ"
BASE COUNT         1125 a         1506 c         1590 g         1133 t
ORIGIN      
        1 cttgaccagt cgcgcgagat cctgcttctg gttgacccgg tgacgctggc catttgtgag
       61 gtcagcaagc ctggcctgcg ccagcttggg tacacgcttg aagagctgct gggccgcccc
      121 attaccgaca tcgaatgcgc gctgtctgac ctgttctttt gggacgaggt tcgacaaggt
      181 ggcaatccca acatcacgga caccgagtgc tcctacctgt gcagcaatgg tgaaattctg
      241 ctggcctcca aaaccgtgtt gcgcgttgag agcgacggca ccagttggct tgtgattcga
      301 gccgagcccc tggagccgcg ccagcaagcc gaaagcgagt tggccagcat cagctcacgc
      361 ctgcaggcca cgctggaggc cacagccgag ggcattttgc tgctggatcg tgagggcagg
      421 attctcaaca tgaaccagtg cttctcgagg atgtggggca ttccggatgc gctattggcc
      481 cgccgagtgg acgaagaggc cttcgccttc atggccagtt tttttgcgga ccaagaggcc
      541 taccagcgta acctggcggc cattcacctg gactccgacc tcgagtcgtt tgacacgctg
      601 gagctgacag atggccgcat ttttgagcgc cgttcacgcc cggcacgcta tgcagagcaa
      661 atcattggac gggttttttc gttcagcgat gccacgcagc gcaaggccat tgaacagcaa
      721 ttactcaacc agaacgcccg gctggaagaa gtggtgcaac aacgcactgc cgcgctgagc
      781 cagcaactgg cggcgctgac cgaatccaac cgcaagctcg aagaggccca gtttcaactg
      841 ctgcaatctg aaaagctggc gtccatcggc caactcgccg ctggcgttgc ccacgagatc
      901 aacaacccga ttggcttcat caactcgaac ctgggcacac tgggcgacta cgtggagtcg
      961 ctgctcgcca ttgatgctgc ttacaaccaa gtgcaacatg ccctggatcc cagtgtgcaa
     1021 ctggcctttg agccagtgaa tgcgctgaaa atcagcctgg actacgcctt catggtggat
     1081 gacctgcgcc agctcatcaa agagtcgcgg gacgggctgg ggcgcgtcaa aaccattgtg
     1141 cgtgacctga aggacttcgc ccgtgtgggg tcggctgaat gggcctggag cgacattcac
     1201 caagggttgg aaagcacatt gaacatcgtc tggaatgaac tcaagtacaa ggcgacggta
     1261 gagcgcgact acgcaacgct gccccgggtt cactgcattc cgtcgcaact gaaccaggtt
     1321 ttcatgaacc tgttggtcaa cgccgcgcac gccattgaag gccagggcgt gatccgggtt
     1381 cgcactgggt gcaatgacaa acaggtgtgg gtggaggtga ttgatacagg aatcggcatt
     1441 ccaccaaaaa atctcaatcg catcttcgac ccgttcttta ccaccaaggc gttgggcaag
     1501 ggcaccggtc tggggctgtc actggcttgg ggtattgtgc agcgccaccg gggaagcatt
     1561 gaagtgcaat ccgagcccgg caagggcacc accttcaagg tgttgctgcc catcggtggt
     1621 gaaccggctg aggcacagcc atcttctcaa tagacgccaa ccaatgacac gccggtcacc
     1681 gccctgatcg gggcggggtt ggctagcgct gacggttata cccaaagggt cgacaccgag
     1741 agtcggcgta gcgtgtttta ttgtcgccgc aacttatatc attattgtag ctactagcgc
     1801 ttattctacg ggggctgtgg cctgttttga tgcattatcg tgagactgcc catggttgtc
     1861 aggcaaggcg tatcgtctaa actaaacagc aacgttgttg cagtggacga ttggccgatg
     1921 aaagaaaaca caatgtagaa agaccgcgat gcaagcaagc cttttcggac agcagttgac
     1981 tttgatggag gaactcaaag ctgagacgtt tgggtcccat gcccggttgc aaacggcccc
     2041 tttctttcag gcattggcgg gctgccattt gccgctcgaa tcctacgtgg gacaactgcg
     2101 ggctttgctg gcaattcatg cagtggtcga acaggccgtg atcgactgtg ttgacgaccg
     2161 agtcggttcg gtctttagcg aagacatgtg caaactaggc ctgctgcgaa aagacctgca
     2221 gtacttcgag ccgcgcaccg tggcagacct caaggtggcg gtcgatgtgg cgcagcagac
     2281 aaccagcgcc ctgcgagagc aatccatgga ggcaccgctg gcggtattgg ggtggctgta
     2341 tgttctggag ggctccacgc tgggcaccac tgtgttgcaa cctctcgtgg cgcgcgcctt
     2401 tttgttggtt ggcgatgacg gaatggccta tctgcgcaac tatggagcgg cagcgcgctc
     2461 ccgatgggct gagtattcgc aacgtatgaa cacgcttgcg ctggaaaccg gagagcgcgc
     2521 acagattgcg caggctgcca acgagctatt ctcccgtctg gaggcgctgt ttcgcgcgtt
     2581 gtacccgttc cagcatgagt ccaaagccta tctggctgcc tccatcaatc cggaagccgg
     2641 acggcatcct gtgcccgatg acgtgcgtga ggtgcatgcg tccgttcggg cgggtgatat
     2701 ttgctggcat cgttttcctt atttcgctgc gcgttatggc gaaagggggc tgggctttgc
     2761 ccgcagcgat gccgcctggc aggcgacgct ctacccatat ccaccggcac aactcctgca
     2821 gcaggtgcaa tggctgggtc gcctgctgtc tgcacgaggc atgcccaccc tgttgttgca
     2881 ggttcaactg gaaattctgg tcgaagagct ggccacggcg attcccgagc gaagggcaga
     2941 ttatgaaaat ctgcttcctg ccgctgctgc gctgcacgca gcgcgctcaa ggttcatcac
     3001 agatgcacaa gcgcaggaac tggccgcagc gtttgatctt gcggtgggcc ccgaatggcg
     3061 tgcaaggttt cctcactccg ggtctttgct caccgcagca gtggccgacg agtgcggggg
     3121 gtgcgagaag gcggttgaaa gtttacgcac ttggatgacc gacgtgcggc agtttccaac
     3181 cgcatggatt gatgcggtcg agagcaccct ggcgcaggcc agacagcagg ccgcgcagaa
     3241 ggcaaagaca tgaacaccgc gatcgacgaa gccacctacg cgcattacct cgccgcgctg
     3301 atagccggtg accgcgaaac gtgcgccagg acggtgcagg cactgctgca cgctggcacg
     3361 ggactcaagc cgctgtatgt tgggctcttg caacgcgccc aataccaggt tggcgagctt
     3421 tgggaacaag aaaaaatttc ggtagccgta gagcatttgt ccaccgccat tacccagcgc
     3481 atgctgtcac tgatacaggc acatacgcta caaggcgcaa gacgcaaccg gagcatcgtc
     3541 gtcgcctgtg tggcagacga gtttcaccag ctcggtggcc ggatggttgc cgatttttgc
     3601 gaattgcgcg gctggcgtgg ctactttctt ggtgccaaca cgtcagttga agatttgctg
     3661 aaactgatct cgcagcgcaa gcctcatttg ctgggtttgt cgctgagcat ttacttcaac
     3721 cttcccaagc tcattgcagc gcttgatgcg gtcacggccg cttaccctga tttgcccatt
     3781 ctggtcggcg ggcaggcctt tcgctggggt gcgcaggatg cgctgcagca ttaccctcat
     3841 gccgcctata tggaatctct ggatgcgttg gaagaacggt tggcggcctg tgagcgctga
     3901 tgccaccctg taccccgagg gctccggcag gcattttctg gcagccagcg ccagcgttgt
     3961 tgtggcgcgg gtcaatgcgt cgggaaccct gctcgccacc aatcagcatg ctgtgaacct
     4021 gatcggcgag ccgctggtgg gaaagccctg gcacaccatg ctgctcaatt ttggggggcc
     4081 gccggtgctg caggaatggc ttgccaaacc tgcgcggcca cgccttttga atatcaagac
     4141 cgcagccggc ttgccgcaga cgctggaggt gacagtggag cccgatggcg cggattacct
     4201 tctgtttggc gaagtgaatg caactgagca ggcgcggcta agccgcgaag tgctgcaact
     4261 caaccacgag ctcaacaata tgagccgcga actcgcgctc aaaaacgacg atctggtcaa
     4321 gacccagagc caattggttc agtcagaaaa gctggcgtct attggactgc tagccggtgg
     4381 agttgcccac gaaatcaaca acccattggc ggtcgtcaaa tccaatctgg gccaattgag
     4441 acaatacgtc aaaacgctgc taacggtcat ccgtgcctct gatgcaattg aagcggatgc
     4501 ttcaggttca tcatcagcat tggccgccct gcaatccctc aagcaggaga gcgacttacc
     4561 tttcatcaag aacgacgtgg tattcctcct cgcagaatgc gatgagagcg tcgatcgggt
     4621 ggagaagatc gtccgtgggc tcagggattt ttcgcgcatg gatgtcaggg aaaccttggc
     4681 gttcgaggac attcaccagg ctctggagag cacgctcacc accatcggtc cagaactcac
     4741 aacccggtgt gagatccgca aggaatatgg cgacatagta cccgtgcagt gcatgcgcgg
     4801 cgagatatgc caggtcttca tgagcctgct ggtcaacgcc atgaaagcca tgaaggaccc
     4861 aggcgtgatt accatttgta ccgggcaaga gaaatcagag gcccggatcg agatttccga
     4921 cactggtcat ggcatagccc aaacggattt gcccctgatc tttgaccctt tcttcacgac
     4981 ccggccggtg ggtcaaggca ctgggctggg cctggcggtt gcttatgcca ttgtccagcg
     5041 gcatcatggc cgaatcgagg tggctagcgc cttaggtgcc ggcaccacgt ttcgcgtctg
     5101 gttaccaatt gtgcaaccgg tcgaagttgc gccaacccaa tagacgttac cgcacgcttc
     5161 gtgaaatttt cactccgtac ttcgctgccc acttaacttg gatgacccgt gtaacattga
     5221 gctgcgcatt caaaaaggca gggacgcgga ctttgcgcgg ttctgtcgca ttaatcggga
     5281 gttttcaata ttttgacgtt tttttcagcg agaacactcc ccggcgcact tatgctgcca
     5341 gtgcataaaa ctgt
//
LOCUS       DAOSLL010000304         5337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13770_length_5337_cov_3.204279, whole genome shotgun sequence.
ACCESSION   DAOSLL010000304 DAOSLL010000000
VERSION     DAOSLL010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13770_length_5337_cov_3.204279"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1899)
                     /locus_tag="PLB25_13160"
     CDS             complement(<1..1899)
                     /locus_tag="PLB25_13160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE42564.1"
                     /translation="MTQNRVTKLDQTPDFAVLQQPASRHTGRNRRALLWLVGLLALMV
                     GSVVTLVLYLNNFEAGEAARRRAADALWLEQSVQFHFRRLEDDLLVLARQAVLQSRNS
                     ADKTMPANALQGGLLWREPGVLLSSGWIAAGQLNDLRVGPERWQLDREAHNENAQSLA
                     TMQTTTRGLRRSAYAGLMLRADGTTTDVLWLAVPFFDRGQFAGNYLAAISLERAVAAL
                     VPQWFMQDHQVQLAADVAAPQNGAAAAGRQQETSYKVALNLPGVDLSLQVQLTQDEPA
                     TVPRIFFLMALLFLLGMLVSLWALRRDIVKRQQVQALLQTQVALRTAMENSVTIGLRA
                     WDLQGRILYVNDAFARMVGFSVSELIGLSTAFPYWPSDQQESLVRVHAGILEHGTSDD
                     GVEVQFAHRDGHLIDVLIHEAPLHTASGEQIGWMSSVLDISERKRSQRMAAQQQEKLE
                     ASGRLVAVGEVASTLAHELNQPLGALSSFANGLLNRLKDHNISLSDLTAVVERMARLA
                     ERAGSIIRRVNDFARRRELSRQRLDLAAFLSRVLASAGDGATVQPHWLAPDASVWIDA
                     DELLLEHLVNNLLANAQDWAGLASHSLNGPQVWVELRQVSTQGMAVLAVADTGPGVSE
                     DAMASVFNA"
     gene            1934..2965
                     /locus_tag="PLB25_13165"
     CDS             1934..2965
                     /locus_tag="PLB25_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOE42565.1"
                     /translation="MSTHDLSSPIDRRQCLGALAATAMVPSLCQAERRPVIVLRFSHV
                     VADETPKGLAANYFKVLVEQRSSGRIAVRVFPNAQLYGDHDEMQALQLGAVDMVAPSL
                     SKFGRIGLPEFELFDLPFLFDNLTAVRRITLGPLGQRLLRGLERQGLVGLGFFDNGFK
                     QMSANRPLLEPKDFAGLRMRVQASQVISAQMRALGARPVTLAFSETRRALATGVVDGT
                     ENPISNFWTQAMHEVQSDLSLTEHGYLGYAVVTNQRFWQHLHPADRALINQALQESLS
                     YANQIADDQNDQALDTLRLTGTTRIHTLTDSQRAVLRQTVRPVHQQLAQRIGTNWTRE
                     LQAALAQRP"
     gene            complement(2986..4479)
                     /locus_tag="PLB25_13170"
     CDS             complement(2986..4479)
                     /locus_tag="PLB25_13170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOE42566.1"
                     /translation="MDVWNHLINGFATAGTPANLMWAFVGCALGTATGVLPGIGPATA
                     VAMLLPITAKVDATASMIFFAGIYYGAMYGGSTTSILLNTPSNSSSMVTAMEGNKMAK
                     SGRAGAALATSAIGSFIAGTFATVVVTLFAPMLARYAVQLGPPEYFMLMLLAFTTVSA
                     VLGQSTLRGLVALFIGLGIGLVGMDQITGQARYTGGVPELLDGINIVLVAVGLFAVAE
                     AMHVVLFEGKQIRHEHKLTRVTMSRADWRRSIPAWIRGTAIGAPFGCIPAGGTEIPTF
                     LSYAVEKNLAKGSNREEFGTVGAIEGVAGPEAANNATVTTAMIPLLTLGIPVSNTTAV
                     MLGAFQNYGIQPGPQLFTNSAALVWALIASLYIGNVMLLVLNLPLVGLWVKLLKIPRP
                     RLYAGILIFSTVGAYGMRQSMFDLFMLYGIGVLGLVMRRFNIPSAPVVVAMILGPLAE
                     AQLRNAMSIGEGSAMVFLQRPVSVIMIFVVIGVLVLPRILKKRGVVL"
     gene            complement(4485..4997)
                     /locus_tag="PLB25_13175"
     CDS             complement(4485..4997)
                     /locus_tag="PLB25_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter TctB
                     family protein"
                     /protein_id="HOE42567.1"
                     /translation="MLDDRSRRYQTAIAVGTMLLALAMGYVALSIPSDAGYAGVGPNF
                     LPWVVSVSLLLCSGFMIYEVRTGGFREMEEASSGDAHPFWLGFAWMSAGMLGGAALID
                     TIGFVLSCTLCFVFAARGLREAEGESLRGWKPWFTDVISGLLISAPVYWVFGMFLAIK
                     LPGLTQSGWL"
     gene            complement(4997..>5337)
                     /locus_tag="PLB25_13180"
     CDS             complement(4997..>5337)
                     /locus_tag="PLB25_13180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter
                     substrate-binding protein"
                     /protein_id="HOE42568.1"
                     /translation="EMSEYIKAGKMRPIGVTSEKRLAGNNTPTLKEQGYDIVLGNWRG
                     VYGAPGITAEQRAALTDIVVKATKSKAWTESLTKNDWTPALLTGKAFDDFVDSEFSSL
                     RGIMHLAGMI"
BASE COUNT         1187 a         1672 c         1482 g          996 t
ORIGIN      
        1 agcattgaaa acacttgcca ttgcgtcttc gctcacacct gggccggtgt cagcaacagc
       61 gagcacggcc atgccctgcg tgctgacctg acgcaattcc acccagacct gaggcccgtt
      121 caggctgtgg cttgcaagcc cggcccaatc ttgcgcgttg gccagcaggt tgttgaccag
      181 atgctccagc agcagctcat cggcatcaat ccagaccgac gcatcaggcg ccaaccagtg
      241 cggttgcact gtagccccat ctccagcgct ggccagcacg cgcgaaagaa aggcagccaa
      301 gtccagccgt tggcgggaca attcacgacg gcgggcaaaa tcgttgacac gtcgaatgat
      361 gctgcctgcg cgctcggcca gtcgcgccat gcgctctacc accgcggtga gatcggacag
      421 gctgatgttg tggtctttca ggcggttgag cagtccattg gcaaaactgc tgagggcgcc
      481 cagcggctga ttcaactcat gagccagggt ggaggccacc tcacccacgg ccaccagtcg
      541 ccccgatgct tccagctttt cctgctgctg ggcggccatg cgttgagagc gtttgcgctc
      601 actgatgtca agcaccgagc tcatccagcc aatttgctcg ccagatgcgg tatgcagcgg
      661 cgcctcgtga atcaggacat caatcaggtg cccatctcga tgggcaaact gcacctcgac
      721 gccatcatca gaggtgccat gctcaagtat gccggcatga accctgacca gcgactcctg
      781 ttgatccgac ggccaatagg ggaatgcggt ggacaggcca atcagctcgc tcacgctgaa
      841 gcccaccatg cgtgcgaacg cgtcattcac atacagaatg cgaccctgaa gatcccaggc
      901 acgtaaacca atggtgaccg agttttccat ggccgtacgc agcgccacct gcgtttgtag
      961 cagagcctga acctgctggc gcttgacgat atcgcggcgc aatgcccaca aggacaccag
     1021 cattcccagc aagaacaaca acgccatcag aaaaaagatg cgcggcacgg ttgcgggttc
     1081 gtcctgcgtc agctgcactt gcagcgagag gtccaccccc ggcaggttca gtgccacctt
     1141 gtaacttgtc tcttgttggc gcccggctgc cgcagcaccg ttttgcggcg cggcaacgtc
     1201 agcggccagt tgaacctgat gatcctgcat gaaccactgg ggcaccaaag cagcgacagc
     1261 acgttcgaga gaaattgccg ccaggtaatt tccggcaaac tggccccggt caaaaaaggg
     1321 cactgccaac cacaagacat cggtggtcgt gccgtcggcg cgtagcatca agccggcata
     1381 ggccgaccga cgcagacccc gggttgtggt ttgcatggtc gccagcgact gcgcgttttc
     1441 attgtgcgcc tcacggtcaa gttgccagcg ctccgggcca actcgcaagt cgttgagctg
     1501 accggcagca atccagccgc tggacaacag tacgcctggc tcacgccaca gcagcccgcc
     1561 ttgcaaggcg tttgcaggca tggtcttgtc agcggaattg cgagattgca gcaccgcctg
     1621 gcgcgctagc accagcaagt cgtcttccag acggcgaaag tggaactgca cactttgctc
     1681 cagccacaag gcatcggcgg cgcggcgacg agcggcttct cctgcctcga aattgttcag
     1741 gtacagcacc agcgtgacca ccgaacccac catcagggcc aacaagccca ccaaccacag
     1801 caaggccctt cgattgcgcc cggtatggcg cgacgccggc tgctgcaaca cggcaaagtc
     1861 cggtgtctga tcaagctttg tgacgcggtt ttgagtcatg tgttgggcag ttcgtccccg
     1921 cataatagcc agcatgtcaa cccatgacct gtcgagcccg attgaccgtc gtcaatgtct
     1981 gggcgccctg gctgcaactg ccatggtgcc aagcctctgt caggctgagc gcaggccggt
     2041 catcgtcttg cgtttttcac acgtggtggc tgatgaaacg cccaaaggtc tggcggccaa
     2101 ctatttcaaa gtcctggtgg aacaacgctc gagtgggcgc attgcagtcc gggtgttccc
     2161 caacgcgcag ctatatggcg accacgatga aatgcaggcc ctgcaactgg gtgcggtgga
     2221 catggtggca ccgtcactgt ccaaatttgg tcgcatcggt ctgccggaat ttgagttgtt
     2281 tgacctgccg ttcttgtttg acaacctcac tgccgtgcgg cgcattaccc tgggccccct
     2341 ggggcaacgc ctgctcaggg ggctcgagcg tcaaggcttg gtggggctgg gcttttttga
     2401 caatggtttc aaacagatga gcgccaaccg gcctttgctg gagccaaagg acttcgccgg
     2461 tctgcgcatg cgggtgcaag cctctcaggt gatttccgct cagatgcgtg cattgggcgc
     2521 caggccggtg acgctggcct tcagcgaaac gcgtcgggcg ctggccacgg gcgtggtgga
     2581 cggaacagaa aatccgattt cgaacttctg gacccaggcc atgcacgaag tgcaaagcga
     2641 cctgagcctc accgagcacg gttacctggg ctacgcggtg gtcaccaatc agcgtttctg
     2701 gcagcacctg cacccagctg accgcgccct gatcaaccag gcattgcaag agtcgctgag
     2761 ttatgccaat cagattgccg acgaccagaa cgaccaggca ctagacactc tgcgcttgac
     2821 cggcaccact cgcattcata ccctcactga cagtcagcgt gctgtattgc ggcagacggt
     2881 gcgcccggtt caccagcaac tggcccagcg cattgggaca aactggaccc gggagctgca
     2941 ggcggcgctc gcgcagcgcc cctgattcag aaaccactgt tctccctaga gcacaacgcc
     3001 gcgttttttc aagatccggg gcaggaccaa aacaccaatc acaacgaaga tcatgatgac
     3061 cgagaccggg cgttgcagaa acaccatggc actgccctca ccaatcgaca tcgcgttgcg
     3121 aagctgcgct tcagcaagcg ggcccaaaat catcgcaaca accaccggtg cggacgggat
     3181 gttgaaacga cgcataacca ggcccagcac cccgatgcca tacagcatga acaggtcaaa
     3241 catgctttga cgcatgccat aggcccccac agtggagaaa atcaaaatac ctgcgtacag
     3301 ccgaggacgt ggaatcttga gcagtttgac ccacaagcca accaagggca gattgagcac
     3361 cagcagcatt acgttgccaa tgtagagcga cgcgatcaac gcccagacca atgccgcaga
     3421 gttggtaaac aactgtggac cgggctggat accgtagttc tgaaatgccc ccagcatcac
     3481 cgccgtggtg tttgacaccg gaatgcccag agtcagcagg gggatcatgg cggtagtgac
     3541 cgtggcattg ttggccgctt ctgggcccgc cacgccttca atggcgccta cggtgccaaa
     3601 ctcttcccgg ttgctgcctt tggctagatt tttttcaacc gcgtagctca aaaaggttgg
     3661 tatctcagtt cccccggccg ggatacagcc aaacggcgct ccgatagcag tgccacgaat
     3721 ccaggccgga atggagcgac gccaatccgc gcgcgacatg gtgacccgcg tcagcttgtg
     3781 ctcgtgccgt atctgtttgc cctcaaacag caccacgtgc atcgcttcag ccacggcaaa
     3841 aagtcccacg gccacaagca cgatgttgat gccgtcaagc agttccggaa caccacccgt
     3901 gtagcgcgcc tggcccgtga tctggtccat tcccaccagt ccgatgccga gtccgatgaa
     3961 cagtgccacc agtccgcgca gggtactttg ccccaagacc gcactcacgg tggtgaaagc
     4021 cagcagcatc agcatgaaat attcgggcgg cccaagttgc actgcgtagc gtgcgagcat
     4081 cggcgcaaac aaggtaacca caaccgtggc gaaggtaccg gcaataaacg atccaatggc
     4141 tgatgtggcc aacgcagcgc ccgcgcgccc gctcttggcc atcttgttgc cctccatggc
     4201 ggtcaccatg cttgaactgt tgctgggcgt attgagcaag atcgacgtgg tcgaaccgcc
     4261 atacatggca ccataataaa tgcctgcaaa aaagatcatt gacgcggtgg catccacctt
     4321 ggcagtgatt ggcagcagca tggccaccgc tgtggccggg ccaatacccg gaagtacgcc
     4381 agtggccgta cccaaggcgc agcctacaaa ggcccacatc aggttcgccg gcgtacctgc
     4441 ggtggcaaag ccattgatta aatggttcca gacgtccatt tccattaaag ccacccactt
     4501 tgcgtcaaac ctggcagctt gatggccaga aacatgccga aaacccagta caccggcgct
     4561 gaaatcagca agccactgat cacatccgtg aaccacggct tccagccacg cagggattcg
     4621 ccttcagctt cgcgcagacc gcgcgcagca aagacaaaac atagcgtaca actgagaaca
     4681 aaaccgatcg tatcgatcaa cgctgcacca ccgagcatgc ccgccgacat ccaggcaaat
     4741 cccagccaga atgggtgcgc gtcgccactg gaggcctctt ccatttcgcg aaatccgccg
     4801 gtgcggactt cgtaaatcat gaagccgctg cacagcagca aggatacgga aaccacccag
     4861 ggtagaaaat tgggcccgac gccagcgtag cccgcatccg acggaatgga cagggccaca
     4921 tagcccatcg ccagggccaa cagcatcgtc ccaacggcga tcgccgtttg gtaccgacgg
     4981 cttctatcgt ccagcatcag atcattcctg ccaaatgcat gatgccgcgc aggctggaga
     5041 actcgctgtc gacaaagtcg tcgaacgcct tgccggtcag caaagctggc gtccagtcgt
     5101 tcttggtgag agattcagtc caagccttgg acttggtagc tttcaccaca atgtcggtga
     5161 gcgcggcgcg ttgctcggcg gtaatgcctg gcgcgccata aacaccgcgc cagttgccca
     5221 gcacaatgtc atacccttgt tctttcaaag ttggtgtgtt gttgcctgca agtcgctttt
     5281 ccgatgtcac accgataggt cgcatctttc cggctttgat gtattcactc atctcac
//
LOCUS       DAOSLL010000305         5331 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13794_length_5331_cov_4.517248, whole genome shotgun sequence.
ACCESSION   DAOSLL010000305 DAOSLL010000000
VERSION     DAOSLL010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13794_length_5331_cov_4.517248"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="PLB25_13185"
     CDS             complement(<1..663)
                     /locus_tag="PLB25_13185"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE42569.1"
                     /translation="MEQTCWVPDVLDGFEQCRLSGDSKSNVRDAPAAGVLVRRRCTRP
                     SAKAVLYIHGYVDYFFQTHLADFYNQQGLHFYALDLRRHGRAMRPHQLPNYTSDVNEY
                     LQDVDAAIEVLHSTEQIHWLLLNGHSTGGLVAALYAHRGAHRSAVNAVFLNSPFLDMN
                     LPAWQQWILEPVLAAIGRWFPRLRLPALSVIYGQSLHADHHGAWQYNTDWKPIAGFPV
                     YAG"
     gene            complement(881..2137)
                     /locus_tag="PLB25_13190"
     CDS             complement(881..2137)
                     /locus_tag="PLB25_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOE42570.1"
                     /translation="MQHATIDHTPKLSAPSLIARQPIVDLQRQVVAYELFDRSTGQDG
                     HSTQSDIFFIFSAMNDVGDQTLISPMLVFINRTHQSLMGGHLDLLRHDKIVLEIGPVP
                     GHAKEDIETLAQTLAALRQRGFRLAFKHTVLAPVYAAWQPLADFVKLDLSVIKPEQIK
                     AVVLAAKARTHATLVAEKVETAVQFATLSSLGVALFQGFWVAHPEVTKAKVVSPTHAH
                     VLQLLNLLRNQASTDEIETALKKDAMLGFNLMRLINSCGFGLTREVTSFREAVMLMGL
                     NRMMRWAALLLITSRTNGAAPLFGTMAVVRGRMMELLAINNLPPEEHDSAFLVGLFSL
                     LDKLLGIPINQALQLLALPRSVTDALIDGTGVLGKMLTLTVACEENDDDACARAALDL
                     NFDNHQINMAHMEALVWADNLVGSAK"
     gene            complement(2255..3169)
                     /locus_tag="PLB25_13195"
     CDS             complement(2255..3169)
                     /locus_tag="PLB25_13195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE42571.1"
                     /translation="MRRTTGYLGAPGKRFRRERLLIVGCGDVGLRVAALLRGRLNLLA
                     LTSNLDRVAELRASGIKPLVGNLDDSQSIKRLAGLGTRVVHLAPPPGSGSGDPRTLAL
                     TRTLRRRPLPKSLVYGSTSGVYGDCQGRHVTETIAASASTARAGRRIDAEMVIRRLGR
                     GSALRCSILRIPGIYAPDRAGGTPQGRLLRGTPVLSAADDVYTNHIHADDLARACLAA
                     LWRGKPGRVYNINDDTCLKMGDYFDLAADLYHLPRPARVSRSMAQQTLPALLLSFMSE
                     SRRLDNQRMKIELGLRLRFPTVLAGLQG"
     gene            3268..4335
                     /locus_tag="PLB25_13200"
     CDS             3268..4335
                     /locus_tag="PLB25_13200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-6-deoxy-delta-3,4-glucoseen reductase"
                     /protein_id="HOE42572.1"
                     /translation="MTIETSAPPNFQVTLEPSGRTFAVHGGETILAAGIRHHINLPYG
                     CQDGACGSCKCKMLSGSVTHGAHQSKALSDEEAGQGLILTCCATPASDVVLEARQVTS
                     EGAFAIKKMPTRVTLLEKKSPDVMRLLLQLPASEVFQYHAGQYVEFLLPGGLRRSYSM
                     ANAPHTQAASPGLELHLRHMPGGKFTDQVFGSLKEREILRIEGPYGSFYLREESSKPI
                     ILLASGTGFAPIKALIEHLQFKGSSRPATFYWGGRRPSDLYMDDWVRAKLLEMPSLRY
                     VPVVSDAQPQDGWSGRTGFVHLAVLQDFADLSGFQVYACGAPVVVDSARRDYLLAGLA
                     EDAFFADAFTSEADKVGAARS"
     gene            4355..5326
                     /locus_tag="PLB25_13205"
     CDS             4355..5326
                     /locus_tag="PLB25_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter substrate
                     binding protein"
                     /protein_id="HOE42573.1"
                     /translation="MNRKTFIAAALVLVAAATAPQVQAQGKPIRLIVPYAAGGPLDVT
                     ARALAERVKDTLGVVIIENKPGAGGNIGADAVAKAAPDGLTIGIAATATHAVNPWLYA
                     KMPYNAATDFAPITQMVRVPNVLVMNADTAARLKINSVRDLIAYARLSPGKLNYGSGG
                     NGSAGHLAGELFKKEAGIFVVHIPYNGGNPAQLALLGGQVDFNFDNLATAAPNIKAGK
                     LKALAVTTLASVAALPGVPPLADTLKGFSVDTWWGLVAPAATPKEVVARLNQAFVAAL
                     NAPETKSRFATLLAEPVPSTPEAFGTLMANERRKYEAMVKASGAKVD"
BASE COUNT         1059 a         1582 c         1651 g         1039 t
ORIGIN      
        1 gcccgcataa accgggaacc cggcgattgg cttccaatcg gtgttgtatt gccacgcacc
       61 gtggtggtcg gcatgcaggc tctgcccgta gattaccgac agggcaggca gccgaagccg
      121 gggaaaccag cgtccgattg ctgccagcac aggctccaaa atccattgct gccaggctgg
      181 caggttcatg tcgagaaacg ggctgttgag aaacaccgcg ttcacggcag accgatgcgc
      241 gccacggtgg gcgtacagcg ctgcgaccag gccaccggtt gaatgtccat tgagtaacag
      301 ccagtgaatc tgctcagtcg agtgcaagac ctcaatggca gcatcgacat cttgcaggta
      361 ttcgttgaca tcgctggtgt aattgggcaa ctggtgtggc ctcatggcgc ggccatggcg
      421 gcgcaggtcc agcgcataaa aatgcagccc ctgctggtta tagaagtcgg ccaggtgggt
      481 ttgaaagaag tagtccacat agccatggat gtacagcacg gctttggcag acggcctggt
      541 gcagcgtctg cgaaccagca cgcccgccgc cggagcgtcc cgcacgtttg acttgctgtc
      601 cccgctcagg cggcattgtt caaatccatc gagcacatca ggcacccagc aggtctgttc
      661 catcggtctc atggtcgttg tagcaagccg ggcatgttaa caaggctctg gtttcggtgg
      721 tgcaggaggg ccgagctggc tgatgggcat aaaacacgaa cagatcgaac tcgcgatgca
      781 tgccaagtgc tgaaaatggc gcactgatgg gccgctgtcg gccagcaatg acctcgcgcc
      841 tgcccggcac ggcggcaggc agatgtcatc tcatgctgcc ttacttggct gagcccacca
      901 ggttgtctgc ccagaccagc gcttccatgt gggccatgtt gatctggtga ttgtcaaaat
      961 tcagatccaa ggcggctcgc gcgcaggcat cgtcgtcatt ttcctcacag gcgacggtca
     1021 gggtcagcat tttgcccagc acgcctgtgc catcaatcaa cgcatcagtg acgctgcggg
     1081 gcaacgccag cagttgcaag gcctgattga tcgggatacc cagcagtttg tcgagcagcg
     1141 agaacaggcc gaccagaaaa gcgctgtcgt gttcttccgg gggcaggttg ttgatggcca
     1201 gcagttccat catgcgccca cgcaccaccg ccatggtgcc gaacagcggc gcggcaccgt
     1261 tggttcgaga ggtgatcagc agcagggcgg cccagcgcat cattcggtta aggcccatca
     1321 acatgacggc ctcgcgaaat gaggtgactt cgcgggtcag cccaaaacca caagaattga
     1381 tcaaacgcat caggttgaaa cccagcatgg catctttttt gagcgctgtt tcaatttcgt
     1441 cggtgctggc ctggttacgc aacaggttga gcaactgcag cacgtgcgcg tgagtgggtg
     1501 aaaccacctt ggccttggtg acctccggat gggctaccca gaaaccctgg aacagcgcca
     1561 caccaaggct agacagggtg gcaaactgca cggctgtctc gaccttttcg gccaccagcg
     1621 tggcatgggt tctggccttg gcggccagca ccaccgcctt gatttgctct ggcttgatca
     1681 ccgacaggtc cagcttgacg aaatcggcca agggctgcca ggcggcataa accggtgcca
     1741 gcacggtgtg cttgaacgcc aggcgaaagc cgcgctgtcg caatgctgcc agcgtctgtg
     1801 ccagcgtctc gatgtcttcc ttggcatggc ctggcaccgg cccgatctcc agcacgatct
     1861 tgtcgtggcg cagcaggtcc aggtgaccgc ccatcaggct ctgatgggtc cggttgatga
     1921 acaccagcat tgggctgatc aaggtctggt cgcccacgtc gttcatggcg ctgaaaatga
     1981 aaaaaatgtc actctgggtg ctgtggccgt cttggccggt tgagcggtca aacaactcgt
     2041 aggcgacgac ctggcgttgc agatcgacga ttggttggcg cgcaatcagg ctgggggccg
     2101 acagttttgg ggtgtggtcg atggtggcgt gctgcatgct caatccgggt ttgggctgaa
     2161 ggcacaggca gactcacttg ctgccgcgtc ctgaattggt gtgttttgcg attgatctta
     2221 caaacaattt gcgacaaatg ccagccaagg gttgtcatcc ttgcagccca gccagaaccg
     2281 ttgggaaccg cagacgcagg cctagctcga tcttcatgcg ctggttgtcc aggcggcgcg
     2341 attcgctcat gaagctcagc agcagcgccg gtagcgtttg ttgggccatg ctgcgcgaca
     2401 cgcgggcggg cctgggcaga tggtacaggt cggcggccag atcaaagtag tcgcccattt
     2461 tcaggcaggt gtcgtcgttg atgttgtaaa cacggcccgg tttgccacgc caaagggctg
     2521 ccagacaggc acgcgccagg tcgtcggcat gaatgtgatt ggtgtaaaca tcgtccgccg
     2581 cgcttagcac aggtgtgccg cgcagcagtc gcccctgtgg tgtgccaccg gcacggtctg
     2641 gtgcatagat tccaggtatg cgcaagatgc tgcagcgcag ggcactgccc cggccaagtc
     2701 ggcgaatcac catttcagca tcaattcgcc ttccagccct tgctgtactt gcgctagcag
     2761 ctattgtttc agtaacatga cgaccctgac agtcgccata cacaccgctg gttgagccat
     2821 agaccagcga cttcggcaag ggcctgcggc gcagcgtgcg ggtcagtgcc agcgtgcgtg
     2881 ggtcgcccga cccgctaccg ggcggcggtg ccagatgcac cacccgtgtg cccagtccgg
     2941 ccagtcgctt gatagattgc gagtcgtcaa ggttgcccac caggggcttg ataccgctgg
     3001 cgcgcagttc cgccacccgg tcaaggttgc tggtcagcgc cagcaggttg agccgaccac
     3061 gcagcagcgc cgccacccgc aggccaacat cgccgcagcc cacaatcagc aggcgttcgc
     3121 gcctgaagcg cttgcccggc gcccccaggt agccggtggt gcgccggatg cgctgatggc
     3181 cgggttgggc gttgcatgaa agacggcgac ggattgcagg caaaattcag tcctttcaaa
     3241 tttgcacgat gcacaggata agacaacatg accatcgaaa ccagcgcacc acccaatttt
     3301 caggtcacgc tggagcccag tggacggact tttgcagtgc atggcggtga aaccattctg
     3361 gccgcaggca ttcgccatca catcaacctg ccttacggtt gccaggacgg tgcctgcggg
     3421 tcgtgcaaat gcaagatgct cagcggcagc gttacccatg gcgcgcacca gagcaaggca
     3481 ctcagtgatg aggaagcagg gcagggcctg attctgacct gttgtgcaac accggcaagc
     3541 gatgtggtgc tggaggcgcg tcaggttacc agcgagggcg cttttgcgat caaaaagatg
     3601 ccgacccgcg tcaccctgct ggagaaaaag tcgcccgatg tgatgcgcct gctgctgcaa
     3661 ctgccagcca gcgaggtctt tcagtaccac gccgggcaat atgtggagtt cctgctgcca
     3721 gggggcctga ggcgcagcta ttcaatggcc aacgcacccc acacgcaagc ggccagccca
     3781 gggctggaac tgcaccttcg ccacatgccg ggcggcaaat ttaccgatca ggtgtttggc
     3841 agcctgaaag agcgcgagat tttgcgcatc gaagggccct atggcagctt ttatctgcgt
     3901 gaggaaagct cgaaaccgat tatcttgctg gcctcgggca ccggttttgc accgatcaag
     3961 gcgctgattg aacacctgca gttcaagggc agcagtcgcc cggccacctt ttactggggc
     4021 gggcgcaggc cgtctgacct gtacatggac gactgggtcc gcgccaagct gctggaaatg
     4081 cccagcctgc gttacgtgcc agtggtgtca gatgcacagc cgcaagatgg ctggtccggg
     4141 cgcacgggtt ttgtccatct ggcggtgttg caggattttg ccgacctctc tggctttcag
     4201 gtttatgctt gcggcgcgcc ggttgtggtg gacagcgccc gccgcgacta cctgctggca
     4261 ggcctggccg aagacgcttt ttttgccgac gctttcacct ctgaagccga caaggtcggc
     4321 gctgcacgat cataatttca gccaaaggat gttcatgaac cgcaaaactt tcatcgccgc
     4381 cgccctggta ctggtcgctg ccgcaaccgc cccgcaggtg caggcgcagg gcaagccgat
     4441 tcgcctgatc gtgccctatg ctgctggcgg gccgctggat gtgacggcac gcgctttggc
     4501 agagcgcgtc aaagatacgc tgggggtcgt catcattgaa aacaagcctg gtgccggtgg
     4561 caacattggc gctgacgcgg tggccaaagc cgcccccgat ggcctgacga ttggcattgc
     4621 ggctaccgcc acccatgccg tcaacccgtg gctgtacgcc aaaatgcctt acaacgcggc
     4681 caccgacttt gccccgatca cccaaatggt gcgggtgccc aacgtgctgg tgatgaacgc
     4741 tgataccgct gcccggctga agatcaacag tgtccgggac ttgattgcct atgccaggct
     4801 cagccctggc aagctcaact acggcagtgg cggcaacggc agtgccggtc atctggccgg
     4861 tgagttgttc aagaaggaag ccggcatttt tgttgtgcac attccgtaca acggcggcaa
     4921 cccggcgcaa ctggcgctgc tcggcggtca ggttgacttc aactttgaca acctggccac
     4981 cgcggcaccg aacatcaagg ctggcaaact caaggcgctg gcggtaacca ccctggccag
     5041 tgtggccgcg ctaccgggtg tgccaccgct ggctgacaca ctcaaaggct tttcggtgga
     5101 cacctggtgg gggctggtgg ccccggcggc cacgcccaaa gaagtggtgg cccggttaaa
     5161 tcaagctttt gtggcggcgc tcaatgcgcc agagaccaaa agccgctttg ccaccctgct
     5221 ggccgagccg gtgcccagca ccccggaggc ctttggcacg ctgatggcca acgagcgtcg
     5281 caaatacgaa gccatggtca aggcatctgg tgccaaagtg gattgagttc a
//
LOCUS       DAOSLL010000306         5308 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13880_length_5308_cov_3.642109, whole genome shotgun sequence.
ACCESSION   DAOSLL010000306 DAOSLL010000000
VERSION     DAOSLL010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13880_length_5308_cov_3.642109"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="PLB25_13210"
     CDS             <1..272
                     /locus_tag="PLB25_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42574.1"
                     /translation="LTFEGLQTFLQGNSTLKLPAYYRNVVQFAAKVAEDCNGLQDSDF
                     EALMAQGFSTEEICEIVSVIDLATMFNVYTSTLKLDLDPQYCAIL"
     gene            312..1382
                     /locus_tag="PLB25_13215"
     CDS             312..1382
                     /locus_tag="PLB25_13215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE42575.1"
                     /translation="MSFNASQVDNTTLQLLRYEALFKLIDDISGLDDIQTIARQVTTR
                     WKYFANVAGWHLVLALGDVFKVIDGFDGVAQVSEVSALSVWDQYHAQLRHPRLLVAAD
                     LEERSAMPESLNGKLVSEISVIPFRRGAEVIGWLSTAARHEPFDELDKKFIRLFGRHL
                     ADRLSDLLLRQLALRALTELATHDALTRLLNRGTVIKELASKLAMSRRTGEPLGLIMI
                     DVDFFKHVNDQHGHQAGDNVLREVSSRLQLRTRDGDNLGRYGGEEFLVVLYPCNEPEI
                     AAAAERLRLAVAEKPVSLGGDLSNAIDLTISLGTTWCRGHDTIRMEALLKQADDALYH
                     AKTVGRNCVVQYSAWLDTATRC"
     gene            1774..2820
                     /locus_tag="PLB25_13220"
     CDS             1774..2820
                     /locus_tag="PLB25_13220"
                     /inference="COORDINATES: protein motif:HMM:NF013866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOE42576.1"
                     /translation="MDFSKLRPLACGLWLAVFGGAALSQNVTPATDFKSGPGNGWYSF
                     ASSSPATVVDLIQSSRARPAATTQAELFLPPNLPKDVKSAAVLLVHGSGGVYPELTTY
                     WAKLLNAQGIAAFVIDVFSPRGVKSTADDQSQVTFASDTADAFAALGMLASHPQIDPK
                     RIAVMGFSRGGITAVRSGAVPIIKGAAPAGLRFAAHIALYSGGCAGMLSVTPKPGAFS
                     PEPMLFVHGDEDDYAYLSDCRTYAQRISAAGTPAELVALPGARHKFDLDSPKRVNLPL
                     STKTKEGCPLEYDLLDLKARDRRSGEALSADKVKEISKELCDAKGASVEGNRSARDAA
                     GKAVIDFLKKTLAV"
     gene            2937..3596
                     /locus_tag="PLB25_13225"
     CDS             2937..3596
                     /locus_tag="PLB25_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42577.1"
                     /translation="MLWLIGSCVNPDRARYSCRFDIDLPGLAQMRRNRARDALKHQRD
                     PTKARAFDCATSFGCLFRLEPSEDINLNLLRMLNHPGVRRGAVIDGLVFASMLVVWSV
                     IEWRSPGARNADTGAMALLGPVMFLLYLWLGAVLALGSSLLGAFAGYWAARTRNPFIG
                     AVAGLLAGIVAAGLLAVLFPANAGEVSLVASWFVKLKWLAPPAIVAGVTVGMTELQRP
                     T"
     gene            4253..>5308
                     /locus_tag="PLB25_13230"
     CDS             4253..>5308
                     /locus_tag="PLB25_13230"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOE42578.1"
                     /translation="MKFRQSAPAILIGVVIVVIATVSVVSNLISHRMAASFEETQFVL
                     MGQIMQSKLSGAEGKALAAAESLASMPPVIKAFSEQDRAALLAATQGSFKVLHEKHGI
                     SQAQFHLAPAVSFLRVHNPEKFDDDQSKFRQMVVEVNRANGIRKGIEVTSSGIGIFGS
                     LPLADDAGRAIGSFEMALEFGPLLDELKRAYGFELALFINEKILHETATSLKGDIFNE
                     QNRVGPYVKFYSTHPELLRTLVGSGDINITEDAHYLREANGVPHGVLLQPIYNYAKKQ
                     IGVVAMARDFSATRLADGQAVVWQTLLGVLSAMLLAGAILIVLRGMVTRPLAVLNERI
                     AAMADGAPERELSGAPGW"
BASE COUNT         1041 a         1492 c         1597 g         1178 t
ORIGIN      
        1 accttacctt tgaaggtctt caaactttcc tgcaaggcaa ctccacgctg aagttgccgg
       61 cgtactatcg caatgtggtt caatttgccg ccaaggtggc cgaggactgt aatggcttgc
      121 aagacagtga ctttgaagca ctgatggcgc aaggcttcag cacggaagaa atctgcgaaa
      181 tcgtctcggt gattgatttg gcgaccatgt tcaacgtgta caccagcaca ctgaagcttg
      241 acctggaccc ccagtattgc gccattcttt gagcttcgat taacgccaag gctttacgct
      301 gacctggtgc catgagtttt aacgcgagcc aggttgacaa caccacgctg caactgctgc
      361 gctatgaagc gttgttcaag ctgatcgatg acatttccgg gctcgatgac attcaaacca
      421 ttgccaggca ggtgaccacc cggtggaagt actttgccaa tgtggccggc tggcacctgg
      481 tgcttgcctt gggcgatgtt ttcaaggtca ttgacggttt cgacggcgtg gcgcaggtgt
      541 cagaggtgag tgcgctctcg gtctgggatc aataccatgc gcagttgcga caccctcgcc
      601 tattggtcgc ggctgacctt gaagaacgtt ctgccatgcc tgaatcctta aacggaaagc
      661 tcgtttctga aatcagcgtg attcctttca ggcgcggtgc tgaggtcatt ggctggctca
      721 gtaccgccgc caggcatgaa ccatttgacg aacttgacaa gaaattcatc cggctttttg
      781 gccggcattt ggcggaccgc ctatcggacc tgctgctgcg ccagctagcc cttcgtgcac
      841 tgaccgaact tgctacccac gacgcgctga cccggctgct caatcgcggc acagtgatca
      901 aggaattggc cagcaaactg gcaatgtcca gacgcactgg agagccgttg ggcttgatca
      961 tgatcgacgt tgacttcttc aagcacgtca acgaccagca tgggcaccaa gccggtgaca
     1021 acgtgctgcg cgaggtgtca agtcggctac aactgcgaac ccgcgatggc gacaacctgg
     1081 gacgctacgg cggtgaagaa tttttggtgg tgctttaccc ctgcaatgag cccgaaattg
     1141 ctgctgcagc cgaacgtctg cgcctggccg ttgctgaaaa gccggtgtcg cttggggggg
     1201 acttgtccaa tgccattgac ttgaccatca gtctgggaac cacttggtgc cgcggacacg
     1261 acaccatccg catggaagcc ttgctgaaac aggcggacga cgcgctgtat cacgccaaaa
     1321 cagtcggtcg caattgcgtc gtccagtatt cagcgtggct tgacacagca acacggtgtt
     1381 aagacatccc gcccgttcga gcaggctcag gctggcaccc tgaaacgctg tttggctgcg
     1441 cccaaaccat gttttcagcc gcgcagcgca atggatgaag ctgcaatgtg cctttgcagg
     1501 ctgccagtac ctcggctcat cctgtcgtac cttcaccggg cgggggcctg aggcggcgat
     1561 gcccgatggc agcttgtgac aggacgactt tgttcctgcg gtgtccacac cattgcaccg
     1621 accacggaaa tagcggcaaa tcaacacatc agtggaagcg ccaattaggc cagtcggtgc
     1681 ggtttggcaa cttgtgtttt gccaggtgcc accctaaact gcagattggg agtttgtcaa
     1741 attgattgtt ctgacttgag aggtgcgtga atcatggatt tttccaaatt gcgtccattg
     1801 gcttgtggac tgtggctggc ggtctttggg ggtgcggcct tgtcccaaaa tgtgacgccc
     1861 gcgactgact tcaaaagtgg cccgggcaat ggctggtaca gctttgcttc gagctctccg
     1921 gcgaccgttg tcgatctgat tcagtccagc cgtgcgcgcc cggccgccac aactcaggcc
     1981 gagctatttt tgccacctaa cctgcccaag gatgtgaaat ccgctgcggt gctgctggtg
     2041 catggttccg gtggtgttta ccccgagttg accacctatt gggccaagct tctgaacgcc
     2101 cagggaatcg cggcgtttgt gattgatgtg tttagcccgc gtggcgtcaa atcgactgcc
     2161 gacgatcaga gtcaggtgac attcgcctcc gacactgccg acgcatttgc ggcgttgggc
     2221 atgctggcaa gccatccgca aattgacccc aaacgcattg cggtcatggg attttcacgc
     2281 ggtggcatta ccgccgttcg cagcggcgca gtacccatca tcaagggggc cgcacccgct
     2341 gggctgcgct ttgctgcgca cattgccctt tacagtgggg gctgcgccgg catgctgtca
     2401 gtgacaccca agcccggtgc attcagcccc gagccgatgc tgtttgtaca tggggatgaa
     2461 gatgactacg cctacctgag cgactgccgc acctatgcgc agcgcatcag cgccgcaggc
     2521 acgcccgctg aactggtggc gttgccaggg gcccggcaca agtttgacct ggacagcccg
     2581 aagcgggtca atttgccctt aagcaccaag accaaagagg gatgtccgct ggaatatgac
     2641 ctcctcgacc tgaaagcacg cgaccgccgc agcggcgagg cgctgtcggc cgataaggtc
     2701 aaagaaatca gcaaagagct ttgtgatgcc aaaggtgcca gcgttgaagg caatcgttct
     2761 gccagggacg ctgccggcaa ggcggtgatt gatttcctga agaaaaccct cgcggtgtga
     2821 atttttcggc atcagcaggc aggcggttca ttgatctggc gtgacagctt ggtggcgggc
     2881 gcagtgcact ttccatcgct tcttgccgcc ctttgcaatc ggttccgcac cgcgtcatgc
     2941 tctggctcat cgggagctgt gtcaatcctg atcgggcccg ctactcatgc aggttcgata
     3001 tcgacttgcc tggcctggcg cagatgcgcc gcaaccgtgc cagggatgcc ttgaagcatc
     3061 aacgcgaccc gaccaaggcg cgtgcctttg actgcgcaac aagctttggc tgcttgtttc
     3121 gccttgaacc atcggaggac atcaacttga atctgcttcg catgctcaat caccccggtg
     3181 tccgccgggg ggcggtcatt gacgggctgg tgtttgcaag catgctggtc gtttggtctg
     3241 tcattgagtg gcgctcgccc ggcgcacgca atgctgatac cggtgccatg gccctgttgg
     3301 ggccggtcat gtttctgctg tacctgtggc taggggcggt ccttgcgctg gggtcttccc
     3361 tgttgggggc atttgctggc tactgggcgg cacgcacgcg caaccctttc ataggggctg
     3421 ttgcaggtct cttggccggc atcgttgcgg ccggcttgct ggcggtcttg ttccctgcca
     3481 atgcgggcga ggtgagcttg gtggcaagct ggttcgtcaa gctcaaatgg ctggcaccac
     3541 ccgccatcgt cgccggagtc acagtcggca tgacggagtt gcagcgacca acataggcaa
     3601 gcaaacgtct gacggcattc tggatgacca gcgcaaggtt tgggctgatg cctgtggacg
     3661 gaaattgttc aacaggtggc tgacaagcaa acgttgcgca gagcaagcag cgcagcgcca
     3721 agcccggcct cctgagcgct tgccggcccg tgacatcagc gcttgccatt gttgcgcccg
     3781 caatcgccag tttctgttgc tggtgatgtt gctcagcagc atgttgagct gcatcgacaa
     3841 agggttgaaa ccgctcagat tgggcaggcg aggcgcatcc aggcagagtt tggcaccagg
     3901 catcacagcg aacgcatttt cagccctgtt agctacacta atagaagcac aaaagatagg
     3961 ccagtcgagg gcttgaacct gatttgacgt tgtttgtgcg gtcgttcgcg agttcaaccg
     4021 cacctttgag aacgatgcgg gttgaccgtt ggcaggccaa ggtggcgatt cccaccgtgc
     4081 cggccaccag catgccgttt gcggcgtgtg gtgcggggtt ggcgacgggt agccaggcca
     4141 gaaccgacca gcgctttccc accctagaag gtaggctaac atgaagggca acaataccgg
     4201 gttggctggc ataaacggca caccctcatt tttcgtcgtt ggagatgttg ccgtgaagtt
     4261 tcgtcaaagt gcccctgcca ttctgattgg tgtggtgatc gtcgtcattg cgaccgtctc
     4321 tgttgtcagc aacctgattt ctcaccgcat ggcggcctcc tttgaagaga cccagtttgt
     4381 gctgatgggt cagatcatgc aatccaagct cagcggtgcc gagggcaagg cgctcgctgc
     4441 ggctgaaagc ctggccagca tgccgccagt catcaaggcg ttttctgagc aagatcgcgc
     4501 ggcattgctg gctgcgactc agggcagttt caaagtgctg catgagaagc acggcatcag
     4561 ccaggcgcag tttcatctgg ccccggccgt gtcttttttg cgggtccata acccggagaa
     4621 gtttgatgac gaccagtcaa agtttcgaca aatggttgtc gaggtcaacc gggccaacgg
     4681 cattcgcaag ggcatcgagg tgacaagctc cggtatcggc attttcggta gcctgccact
     4741 ggccgatgac gctggccggg ccattggcag tttcgaaatg gccttggagt tcgggccgct
     4801 gctcgacgaa ctcaagagag cctatggctt tgagctggcg ctgttcatca acgagaaaat
     4861 cctgcatgaa acagcgacat cgctgaaggg cgatattttc aacgagcaaa atcgtgtcgg
     4921 gccctatgtc aagttctatt cgacccatcc tgaattgctg cgcacgctgg taggcagcgg
     4981 ggacatcaat atcaccgaag atgcgcatta tttgcgtgag gccaacggcg tgccgcatgg
     5041 tgtgctgctg cagccgattt acaactacgc gaagaagcag atcggtgtag tggccatggc
     5101 gcgcgacttc agcgccaccc gtcttgccga tggacaggcc gttgtctggc aaaccttgct
     5161 tggcgtgctg tcggccatgc tgctggccgg ggccattctg atcgtgttgc gcggcatggt
     5221 cacgcggccc ctggcggtgc tcaacgagcg catcgcggca atggccgatg gcgcgccaga
     5281 gcgcgagctg tccggtgcgc ccggctgg
//
LOCUS       DAOSLL010000307         5294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13927_length_5294_cov_3.900935, whole genome shotgun sequence.
ACCESSION   DAOSLL010000307 DAOSLL010000000
VERSION     DAOSLL010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13927_length_5294_cov_3.900935"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /locus_tag="PLB25_13235"
     CDS             complement(<1..516)
                     /locus_tag="PLB25_13235"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOE42579.1"
                     /translation="MAPFSDLCLGDLMNRQVTVVSGDSMIASVAAQLKDRPASQVIVA
                     EGKNPVGMLTERDLVRLLRRPTALSALAREVMSAPIVTVSASLGFHPALVQLCLSRIR
                     HLVVVDRLGAVVGVVREGDFLGQLGQDLCQSLQRFESLVDWSVPQLDVTTPVLQAIDC
                     MVQQRRGCTVVC"
     gene            complement(927..1934)
                     /locus_tag="PLB25_13240"
     CDS             complement(927..1934)
                     /locus_tag="PLB25_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE42580.1"
                     /translation="MPDFPGLLGLEISMDEALWGTMLASTLRVSTPLIFCALAGLFSE
                     RSGVVDIALEGKMLLAAFAAGACGAVSGSTSLALLAAMAVAVAMSWLHGLACVSHKGD
                     QVVSGVAINIIAVGLTAVLGAAWFHQGGQTPPVSAEVRIQALFPALADAARALPVLGT
                     VLGDGVLGHNALVYLALLLVPLVWWTLFRTRFGLRLRAVGENPQMVDAAGVSVVRLRY
                     TALTLNGILCGLAGSYLVLALSASFSQNMTAGRGFMALAALIFGGWHPVRALWACLLF
                     GFLDAAAIRLQGVQVPGIGEVPVQVIQALPYVLTVVLLAGFIGKSVAPKALGIPYSKE
                     R"
     gene            complement(1888..3024)
                     /locus_tag="PLB25_13245"
     CDS             complement(1888..3024)
                     /locus_tag="PLB25_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE42581.1"
                     /translation="MNALPRWADLVLLPLVSLLVALLAAGGVVALVGQDPWLVIQVLV
                     QGAFGTARGLSYTLYYATTFVFTGLAVAVAFHGGLFNIGGEGQAIMGGLGSGLVALWW
                     SAYLPPWVMLPLMMLAGALFGLAWAAVPAYLQAYRGSHVVITTIMFNFIASSVLVYLL
                     VNHLRPKGSMSVESAEFAASAKLPGMHQALAWLGVDWPASPLNLSVLLAALAAVLIYL
                     FLWQTRAGYRLRAVGSSSTAAEYAGIKAKHQVLMAMSISGALAGLVGMNEIAGVSGKL
                     LLEFVSGAGFTGIAVALMGRNHPLGIVLASILFGALFQGGAEVAFEVRGFSRDMVVML
                     QGFIVLFSGAMTYVIAPRLANLLGWVSRPLARLSRSAGSGDQHG"
     gene            complement(3377..4909)
                     /locus_tag="PLB25_13250"
     CDS             complement(3377..4909)
                     /locus_tag="PLB25_13250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE42582.1"
                     /translation="MADSAVLAVRMIGICKHFGAVAANADVDLQVAAATVHGIVGENG
                     AGKSTLMSILYGFYQADSGHIEVLGKPATIRNADDAIALGIGMVHQHFMLVDTLSALE
                     NVMLGAEPDWLLQRAVAQVRRKLTELMQSTGLHIQLDSLVADLPVGDRQRLEILKALY
                     RGARILILDEPTAVLTPQEATQLFAVLKRLREQGTTLILITHKLKEVMALCDTVTVMR
                     AGQVVADVPIASASIEGLAQTMVGRRVNMGRVAGQETVAGEILLQADHLSVTDGLGVQ
                     RLKDVSLTLRAGEIVGVAGVSGNGQSELIDVLTGLLSPRQGRLQLLGRSFESPLWLDP
                     SLARTLGLAHVPEDRQARALVMAFAAWESAALGYDNLPEYATHGWMHHSRMRQATMDM
                     MERFDVRPRDAELVSSKFSGGNQQKLVLARELGRAPKVLLVGQPTRGVDIGAIEFIYG
                     QLRALRQAGCAVLVVSSELDEILALSDRVVVMNQGRIAGELPIADCSEAALGLLMTGG
                     ST"
BASE COUNT         1233 a         1792 c         1425 g          844 t
ORIGIN      
        1 gcaaacgacg gtacagccac gtcgctgttg gaccatgcag tcaatcgcct gcagcaccgg
       61 cgttgtgaca tcaagctggg gtaccgacca gtccaccagg gactcaaagc gctgcaggct
      121 ttggcacaag tcctggccaa gctgccccag aaaatcacct tcacgcacca cccccacgac
      181 agcgccgagg cggtcaacca ccaccaagtg tcttatccgg ctcaggcaaa gctgcacaag
      241 ggccgggtga aaaccaagtg acgcagagac cgtgacaatc ggcgcgctca tcacttcccg
      301 ggccagggcc gacagtgccg ttgggcggcg cagcaagcga accagatcac gctcagtgag
      361 catgccgacg gggttcttgc cctctgcaac gatgacctgc gacgcgggcc ggtctttcaa
      421 ctgcgcagcg acgctcgcga tcatgctgtc gccactcacg acggtgactt gacgattcat
      481 caggtcgccc aggcacaggt cagaaaaagg agccatattg cgagcctcag gttcacagag
      541 cacggttcat tctagagagc ctcagcgcca aaccagcttg catctggtgc aagtgattga
      601 ctcagatcag ggaaatgcgc caaaaaaggg cagcttgtcc aaaggcccag cccggcactg
      661 gaacgatgcc cgactggcgg accggcctca ctggctgaat cagacccaaa ggctgtcgcc
      721 gaacacggca tttgcgcaag ccttgcggtg ctgctcatcg gcttcgggct tggcgcaagg
      781 agcgtgtcgg acttaccgcg catcgcctgc gaaccggccg cagcggccca ttgtgcgtcg
      841 gctttcttta gagtgagccc agcaacatct caagtgctct gggcgaatga tttgccacta
      901 ccagggcgca ggctcagacc cggtgatcaa cgctccttgg aatagggaat cccgagtgcc
      961 ttgggtgcca ccgatttgcc gatgaacccc gccagcagca ccaccgtcag cacatacggc
     1021 aacgcctgaa tcacctgcac tggtacctcg ccaatgccag gcacctgcac cccttgcaga
     1081 cgaattgcgg ccgcatccag aaaaccaaac agcaggcaag cccaaagcgc cctgaccgga
     1141 tgccaaccgc caaaaataag cgcagccagt gccatgaaac cgcgccctgc ggtcatattt
     1201 tgagaaaagc tcgcactgag cgccagcacc agataactgc cagcaagtcc gcacaagatg
     1261 ccgttgaggg tcagtgcggt gtagcgcaga cgcaccaccg agaccccggc ggcatcgacc
     1321 atttgcgggt tctcgccaac ggcgcgcagg cgcaggccaa agcgggttcg aaacagcgtc
     1381 caccaaacca agggcaccag cagcaaggcc agatagacca gcgcgttatg tcccaacacg
     1441 ccgtcaccca gcacggtgcc caacaccggc aaagcacgcg ccgcatcagc cagggccggg
     1501 aacaatgcct gaatgcgcac ctcagcacta accggcgggg tctgccctcc ctgatgaaac
     1561 caggctgcac ccagcacggc agttaggccg acagcaatga tatttatagc gactccagaa
     1621 accacctggt cacccttgtg gctcacgcaa gccagtccat gcagccacga catcgccacc
     1681 gccacggcca tcgcagccaa caaagccaag ctggtggacc cagagactgc gccgcaagca
     1741 ccagcagcaa acgctgccag cagcatcttg ccttccagcg caatgtccac cacgccggat
     1801 cgttctgaaa acaaacccgc cagcgcacaa aaaatcaatg gcgtcgaaac ccgcagggtc
     1861 gaggccagca tggtgcccca cagcgcctca tccatgctga tctccagacc cagcagaccg
     1921 ggaaagtctg gcaagcggcc gactgaccca gcccaacagg ttcgccagcc gcggtgcaat
     1981 cacataggtc atcgcacctg aaaaaagcac gataaaaccc tgcaacatga ccaccatgtc
     2041 gcggctgaaa ccgcgcacct caaacgccac ctctgcgccg ccctgaaaca acgcgccaaa
     2101 aagaatgctt gccagcacaa tgcccagcgg gtggttacgc cccatcagtg ccaccgcaat
     2161 gccggtaaag ccagcaccgc tgacaaattc cagcagcaac ttgccactga ccccggcaat
     2221 ttcattcatc cccaccagtc cggccagcgc gcccgaaatc gacatcgcca tcagtacctg
     2281 gtgctttgcc ttgatgcccg cgtactcggc agccgtgctg ctcgacccca ccgcgcgcaa
     2341 ccgatagccg gcgcgggttt gccacaaaaa aaggtaaatc agcacggcgg ccagggccgc
     2401 cagcaacacg ctcaaattca atggcgaagc cggccaatca acccccagcc aggccagcgc
     2461 ctggtgcatg ccgggcaatt tggccgatgc ggcaaactcg gcgctctcca ccgacatgct
     2521 gcccttgggc cgcaaatggt tgaccagcag atagaccagc acactgctgg caatgaagtt
     2581 gaacatgatg gtagtgatca ccacatggct gccccggtac gcctgcaaat acgccggcac
     2641 agccgcccag gccaggccaa acagcgcgcc agccagcatc atcagcggca acatcaccca
     2701 gggcggcaaa taggccgacc accacaacgc caccagcccg ctgcccaagc cgcccatgat
     2761 ggcctgccct tcgcccccaa tattgaacaa accgccatga aaggccaccg ccaccgccag
     2821 cccggtgaag acaaaggtgg tggcgtaata gagcgtgtag ctcaagcccc gggcggtgcc
     2881 aaaggcccct tgcaccaaca cctgaatcac cagccacggg tcttgcccaa ccagcgccac
     2941 cacgccaccc gccgccagca aggccaccag cagactcacc agcggcaaca agaccagatc
     3001 ggcccagcgc ggcaaagcgt tcatcctggc atccccgatt ggccgcgcca acgtggcccg
     3061 ctggcagcgc ggctggtccg ggcgaaagcc gcagcttcaa aggtgccggt gcaggtttgg
     3121 actgctttga gcagacccgc ctggtggcat ggctgcgccc tgcatgtcgc agcaagctgc
     3181 caggggcgac ggcgcgccct actgagtgca tgtcgccctg gcggacgagg caggccaacc
     3241 gcgtccaacc gcattgccag cggttcaatc gggtgggtag cgctgtcacc caatgggtga
     3301 accccatcga agctaaaaaa gtcaatactc ataagaacgt cagatgaaag caagcggcca
     3361 ttcaaggaaa ctgggttcat gtgctgcccc cggtcatcaa caaccccagc gccgcttcag
     3421 aacaatcggc aatcggcaac tcacctgcaa tgcggccctg attcatcacc accacccggt
     3481 cggacagtgc cagaatttca tcgagttcgc tcgacaccac cagcaccgca caacccgcct
     3541 gccgcaaggc gcgcaactgg ccataaatga actcgatggc cccgatatcg accccacgcg
     3601 tgggttgccc gaccagcaac accttgggcg cgcggcccag ttcgcgcgcc agcaccagct
     3661 tttgctgatt gccgcctgag aacttgctgc tgaccagttc agcatcacgc gggcgcacat
     3721 caaagcgttc catcatgtcc atcgtggcct ggcgcatgcg gctgtggtgc atccagccat
     3781 gggttgcgta ttcaggcaga ttgtcatacc ccagcgcagc cgattcccag gcggcaaaag
     3841 ccatcaccag cgcacgtgcc tggcggtcct cgggcacatg ggccagcccc aaagtgcgcg
     3901 ccagactcgg gtccagccac agcgggctct caaagctgcg gccgagcaac tgcagcctgc
     3961 cttggcgggg cgacaacaag cccgtcaaca cgtcaatcag ctcactttgg ccgttgcccg
     4021 acacgccagc cacaccgacg atctcaccgg cacgcagggt cagactcaca tctttcaggc
     4081 gctgcacacc caggccgtcg gtcaccgaca agtgatccgc ttgcagcaat atctcacccg
     4141 ccacagtctc ctgaccggca acccggccca tgttgacccg ccgtccgacc atggtctgcg
     4201 ccagtccctc gatggatgcc gaggcaatcg gcacatccgc taccacctga ccggcacgca
     4261 tcaccgtcac ggtgtcgcac aaggccatca cttccttgag cttgtgggtg atcagaatca
     4321 gcgtggtgcc ctgctcgcgc aagcgtttga gcaccgcaaa caattgggtg gcttcctgtg
     4381 gtgtcagtac agcggtgggc tcatcgagga tcaatatcct ggcgccgcgg tacaaggcct
     4441 tcagtatctc cagccgctga cggtcgccca ccggcaaatc ggccaccagg ctgtcgagtt
     4501 ggatatgcag gccggtggac tgcatcagct cggtgagttt gcgccgcacc tgggcaacgg
     4561 cacgctgcag cagccaatcg ggttcggccc cgagcataac gttttccaat gcgctgagcg
     4621 tatcaaccag catgaagtgc tgatgcacca tgccaatgcc cagcgcaatc gcatcgtccg
     4681 cattgcgaat ggtggcgggc ttgccaagca cctcaatgtg cccactgtca gcctggtaaa
     4741 agccgtacag aatcgacatc agcgtgcttt tgcctgcacc gttttcacca acaatgccat
     4801 gcaccgttgc ggcagccacc tgcaagtcaa catcggcatt ggcagcgacg gcaccaaaat
     4861 gcttgcagat gccgatcatg cgcacggcca ggactgccga gtcagccatg cgagcgggct
     4921 cccgatggcg cgcaacggcg atttatcagt actttagaag tatgcatgtt tttgtgctct
     4981 agaccttgat ggtattgcgc tacaagctat atatttcaga gctatcgaaa gcaattaagg
     5041 tcggcttggt tgtcattgat ggtgacggtt ttgaagcatc gctggcgtgg tccgcagcaa
     5101 tcaggtcaat gcccaattca acgataccgt gcgcgtccat tcaatgacat ctggcagaaa
     5161 tgtgatgcga ccccgattgt gctagcgcag gcccagaaca ggcacgccaa accgcgatca
     5221 atccccaccc aggaactatg gatgtggtca gaaaaaactt cccctatttg caccggcgga
     5281 tttgggcgca cagc
//
LOCUS       DAOSLL010000308         5282 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13971_length_5282_cov_4.499330, whole genome shotgun sequence.
ACCESSION   DAOSLL010000308 DAOSLL010000000
VERSION     DAOSLL010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5282
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13971_length_5282_cov_4.499330"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="PLB25_13255"
     CDS             complement(<1..588)
                     /locus_tag="PLB25_13255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOE42583.1"
                     /translation="MKFKAILFDCDGVLVDSEPIANGVLRDMLEESGWSMTLAECMHY
                     FVGKTVREEATKIALHTGQALTDDWLQRYYQRRNQALIADLQIIAGAQQAVQAAHKEA
                     QARIACASGADRFKTEMQLRKVGLLGYFEGRVFSGHEMARSKPAPDVYLAAASHLKVE
                     PEQCLVIEDSTVGITAAVAAGATVWAYCPLPEAGPK"
     gene            complement(662..2023)
                     /locus_tag="PLB25_13260"
     CDS             complement(662..2023)
                     /locus_tag="PLB25_13260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOE42584.1"
                     /translation="MRCCLRAVAALLLVLLGACSSLRPWQNQPMQPDRAPAALSLPEA
                     SDRSLLLFVSLSGGGARAAAFGYGVLEALQQTRVQWQGRSISLLDELDVISGVSGGSI
                     IAAYYAAFGAQTFPAFEQHFLRQNFQHNLIANMLSPGNLYDLSSPWFGRTHLLERRLN
                     QLFQGKTFGDLAERAGQPRLLVSATDLSLGSSFDFTWRQFSLICSDLSSVPLSFAVAA
                     SSAVPVALSPMTLKNHNDNCPRADLKIKSTDANDYRVRLLHLAQHSYRDAKARPFIHL
                     VDGGLADNLGLRSLLDRSRSDGSLRDSVRRMGSAQVAKMVIIVVNAERDPSERIDASD
                     QVPGTLQVMDSLLFGAGARATQETLGMLSDTAQAWQHELRSAQGQAGDPFEPDAQIHV
                     VNVNLRDAPELMERNLLLQIPTAFSISDSDVSRLMQAGRRILLQSKEFNALIESLGGA
                     LPR"
     gene            complement(2056..2481)
                     /locus_tag="PLB25_13265"
     CDS             complement(2056..2481)
                     /locus_tag="PLB25_13265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOE42585.1"
                     /translation="MPITKGSRALVDEAMAKVKTYSAEQILSRMSDPTLQIVDIRDVR
                     ELEREGTIVGCFNAPRGMLEFWVDPASPYFKQIFGDESKEFVLFCGAGWRSALAAKAL
                     QDMGMTNVAHIDGGFADWVRLGAPIESMDVHRARRHGKS"
     gene            complement(2733..3677)
                     /locus_tag="PLB25_13270"
     CDS             complement(2733..3677)
                     /locus_tag="PLB25_13270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008907187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE42586.1"
                     /translation="MQHCTDLSSGASALPAGITVLERGWLSSNNILIQGDRGAALIDS
                     GYCSHAAQTLALVRSSLRGHPLTLLLNTHLHSDHCGGNAALQAAYAELRTLIPPGLAT
                     HVSQWNPDALSYTPTGQSCPQFRFDALLQPGTELPLGDLLWQIHAAPGHDPHSVILFE
                     PVSRTLVSADALWECGFGVVFPELDGDNAFDEVSATLDLIETLQPVIVIPGHGRPFDD
                     LAGALAVARRRLDGFLKSPQRHLQYAAKVLLKFKLLQAQSLERADLLAWAHGTNYLDA
                     LFSRLFAGQEFSQGLDQLVQELLRAGAATRQGTVLHNA"
     gene            complement(4028..4987)
                     /locus_tag="PLB25_13275"
     CDS             complement(4028..4987)
                     /locus_tag="PLB25_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HOE42587.1"
                     /translation="MQTIAPLWLWFTFGGIVLVSLFIDFVVLKKQGAHDIGVKEALNW
                     SLVWVALSFLFNGLFWWAVKDSTGSAELANTKSLEFLTGYLIEKSLAVDNIFVFLMIF
                     TYFAVPKPFQKRVLMIGIIGAIVLRTVMILLGGWLLAHFHWILYVFGAFLIVTGVKMW
                     WAAGKEPDLNDNPALKLLRKLLPVSKHYDGERFWTLEAGQKIATPLFMVICLIAFTDV
                     IFAVDSIPAIFAITNDPFIVLTSNVFAILGLRAMYFLLAAVANKFHLLNYGLAVILVF
                     IGAKMCLIDFYKIPVAVSLAVVVAILAATMLLSLRTARPEPPH"
     gene            5097..>5282
                     /locus_tag="PLB25_13280"
     CDS             5097..>5282
                     /locus_tag="PLB25_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42588.1"
                     /translation="MFKLIKQPIWFVKTGVISRLCLLLCGASLSVCGPGASGAVRPAL
                     GCGQKQLPLFAPADLGAQ"
BASE COUNT         1179 a         1567 c         1549 g          987 t
ORIGIN      
        1 ctttggcccg gcttctggca gcgggcaata ggcccacacc gtggccccgg ccgccaccgc
       61 tgcggtaatg ccgacggtgc tgtcttcaat caccagacac tgctcaggct ccaccttcag
      121 gtggctggca gcagccaggt acacatctgg tgcgggcttg gagcgggcca tctcgtggcc
      181 actgaaaacc cgcccttcaa aatagcccag caggccaacc ttgcgcaact gcatctcggt
      241 cttgaagcgg tctgcgcccg aggcacaggc aatgcgcgcc tgggcttctt tgtgtgcggc
      301 ttgcaccgcc tgttgcgcac cggcaatgat ctgcagatcg gcgatcaagg cctgattgcg
      361 acgctggtaa tagcgttgca accagtcgtc cgtcagggct tgcccggtgt gcaaggcgat
      421 cttggtggcc tcctcgcgga cggttttgcc gacaaagtaa tgcatgcact cggccaaggt
      481 catggaccag ccgctttctt ccagcatgtc gcgcagcacg ccgttggcaa tcggctcgct
      541 gtccaccagg acaccatcac agtcaaacag gattgcttta aatttcatag ctgttggcgc
      601 atataggata agggctagac ggtgttttga tgtactttat gaagattcgg ggggcactgg
      661 ttcagcgggg cagtgcgccg cccaatgact cgatcagcgc gttgaattct tttgactgca
      721 gcaagatgcg ccgacccgct tgcatcagtc ggctgacatc gctgtccgaa atcgaaaacg
      781 cggtgggaat ctgcaacagc aagttgcgct ccatcaactc gggggcatcg cgcagattga
      841 cattgacaac atgaatttgc gcatccggtt caaacgggtc gccagcttgc ccctgggcgc
      901 tgcgcagttc atgctgccag gcttgtgccg tgtcgctgag catgccaagc gtttcctggg
      961 tggcacgggc accggcacca aacagcagcg agtccatcac ctgcagggtg ccgggtacct
     1021 gatcgctggc atcaatgcgt tcggacgggt cacgctcggc gttgaccaca atgatcacca
     1081 tctttgccac ctgcgcactg cccatgcggc gcacgctgtc acgcaagctg ccatcagagc
     1141 gcgagcggtc gagcaaactg cgcagtccca ggttgtcggc caggccgccg tccaccagat
     1201 gaatgaaggg gcgcgccttg gcatcccggt aagaatgctg ggccaggtgc aataaacgca
     1261 cgcgatagtc gtttgcatcg gtgcttttga tcttcaaatc ggctcgtgga caattgtcgt
     1321 tgtggttctt gagcgtcatt ggactgagcg caactggcac cgcagacgaa gccgccacgg
     1381 caaacgacag gggtacgctg ctcaggtccg agcagatcag tgaaaactgg cgccaggtga
     1441 aatcaaagct cgaacccagc gacaagtcgg tcgctgacac cagcaggcgt ggctgaccgg
     1501 cacgctctgc caggtcgcca aaggtcttgc cctggaacaa ttggttgagc cggcgctcca
     1561 gcagatgggt gcggccaaac cagggcgacg acaagtcata cagattgccg gggctgagca
     1621 tgttggcaat caggttgtgc tgaaagttct gccgcaaaaa atgttgctca aaagcaggaa
     1681 aagtctgcgc accaaaggcg gcgtaatagg ctgcaatgat gctgccaccc gacacgccgc
     1741 tgatcacgtc aagctcgtcc agcagactga tgctgcgccc ctgccactga acccgggttt
     1801 gctgcaacgc ttcgagcacg ccatagccaa acgccgccgc ccgcgcgcca ccgccagaca
     1861 ggctgacaaa cagcaacagc gagcggtcgc tggcctcggg caggctcaga gcggctggcg
     1921 cgcggtctgg ctgcatcggc tggttttgcc acggacgcag cgatgaacac gcgcccagca
     1981 gcaccaacag cagcgccgcc acggcgcgca ggcaacagcg catgcggtca gcgcatcacc
     2041 ggcaagccag tcggctcagc ttttgccatg gcggcgcgcc cggtgcacgt ccatgctctc
     2101 aatcggcgcg cccagcctga cccagtcggc aaaaccgccg tcaatgtggg ccacattggt
     2161 catgcccatg tcctgtagtg ccttggccgc cagcgcactg cgccagccag caccacaaaa
     2221 cagcacgaac tccttgcttt cgtcgccaaa aatctgcttg aagtagggcg aggccggatc
     2281 gacccagaat tcgagcatgc cgcgcggcgc gttgaagcaa cccacaatag tgccttcacg
     2341 ctcgagttcg cgcacatcgc gaatgtcaac aatctgcagc gtcgggtcgc tcatgcgact
     2401 cagtatctgc tctgcagaat aggttttgac cttggccatg gcctcgtcaa ccagggcgcg
     2461 acttcctttg gtaattggca tgattcgact ccttgaaaca acaattgatt ccattcccaa
     2521 atcatccatt ttcttgttat ctcgccttgc cttgaggttg caacgtgtgc ggcagggtgc
     2581 ctggaaacgg gtgatttgaa aacgcaatga ggcattatcc gcgagccctt gtggctcggt
     2641 gtgtcattgc attcccagat gattcgtcta gcagcctgcg ccagacattg caaacgcaag
     2701 gcaagacaac acaaaggggt ttggcaatgg catcaggcgt tatgcaatac cgttccttgc
     2761 cgcgtggcgg caccggcacg cagcagttct tgcaccagtt ggtcgagccc ttgcgagaat
     2821 tcctggccgg caaacaagcg cgaaaacagc gcatcaaggt aatttgtgcc atgcgcccag
     2881 gccagcaggt cggcgcgctc caggctttgc gcctgtagca gcttgaactt gagcaagact
     2941 ttggcagcat attgaagatg ccgctgtggg cttttcagaa agccgtcgag ccgtcggcgc
     3001 gccaccgcaa gtgccccggc gaggtcgtca aaaggtcggc catggccggg gatgacgatg
     3061 acgggttgca aggtttcgat cagatcgagc gtggccgaca cttcgtcaaa ggcgttgtca
     3121 ccgtcaagct cgggaaacac cacgccaaaa ccgcattccc acagcgcgtc ggccgacacc
     3181 agcgtgcggc tgaccggctc gaacaggatc accgaatgcg ggtcgtggcc aggtgcggcg
     3241 tgaatttgcc acagcaagtc acccagaggc aattcagtgc cgggttgcag cagcgcatcg
     3301 aagcgaaact gcgggcagct ttgtccggtg ggggtgtagc tcagggcatc cgggttccat
     3361 tgactcacat gagttgccag ccctggcgga atcagcgtgc gaagttcggc ataggcagct
     3421 tgcaacgcag cattgccgcc gcagtggtcg ctgtgcaaat gggtgttgag cagcagtgtc
     3481 agcggatgac cccgcaagct tgatctgacc agcgccaggg tttgggcggc atgcgagcaa
     3541 tagccgctgt cgatcaacgc cgcgccgcgg tcaccctgaa tcaggatgtt gttggaagac
     3601 agccagccac gttccagcac agtgatgccg gcaggaagcg cagatgcgcc agagctcagg
     3661 tcggtgcaat gctgcatcac tctggatggg tcggctgacc tggcacgcgc gggccgctgg
     3721 cagcgcatgg gtagcgacaa cgcagcgggt tcctgagtcc atgttgcgct ggcggacgag
     3781 ggaagtgcaa cgacccctgg cgcgttgcca gtggctccat cgggtgggtc gcgttgtcac
     3841 ccaatgggtg aaacccatcg acgccgaaaa agtcaatatg catgtggact taccgatgaa
     3901 aacaagcggc caaccgaaga atccaggatc atggtctgac cccgaatata ggtcaaatcg
     3961 gccaatgtca gcgctgctgc gctgacttga cgcaagcgca ctcggtgcga ccgggcaagc
     4021 tagccggtca gtgcggcggt tcaggtctgg cagtgcgcag gctcagcagc atggtggcgg
     4081 ccaaaatggc caccaccacc gccagcgaca ccgccaccgg gattttgtaa aaatcaatca
     4141 ggcacatctt ggcaccgatg aacaccagaa tcacggccag accgtagttg agcagatgga
     4201 atttgttggc gactgcggcc agcagaaaat acatggcccg caaacccaga atggcaaata
     4261 cgttgctggt cagcacaatg aacgggtcat tggtgatggc aaagatggct ggaatcgagt
     4321 ccaccgcaaa aatcacgtcg gtaaaagcaa tcaggcaaat caccatgaac agcggggtgg
     4381 caattttctg gccggcttcg agggtccaga acctctcgcc gtcgtagtgt ttgctcaccg
     4441 gcagcagctt gcgcaacagc ttcaatgccg ggttgtcatt gaggtccggc tccttgccgg
     4501 cggcccacca catcttcacg ccggtgacga tgagaaacgc cccgaacaca tacaaaatcc
     4561 agtgaaagtg cgccagcaac cagccaccca gcaagatcat cacggtacgc agcacaatcg
     4621 cgccaatgat gccaatcatc agcacccgct tctgaaacgg cttgggcacg gcgaagtagg
     4681 tgaagatcat caggaaaaca aagatgttgt ccaccgccaa cgatttctcg atcaggtaac
     4741 cggtcagaaa ttcaagcgac ttggtgttgg caagttcagc cgacccggta ctgtccttga
     4801 ccgcccacca gaacaggcca ttgaacagaa agctcagggc cacccagacc agcgaccagt
     4861 tcaatgcctc tttgacacca atgtcgtgcg ccccctgctt tttcagcacg acgaaatcaa
     4921 tgaacagcga caccagcacg atgccgccaa aggtaaacca aagccacagg ggcgcaattg
     4981 tttgcataac aaccttcaaa gtgtgaaatc agcagcccgt gcggggccgc ttggctggcg
     5041 cggcggacca aagcgccacc gtgcacggca ggcaccaagt ttaaaaggaa acaaccatgt
     5101 tcaagctgat aaaacagcca atatggttcg taaaaacggg agtgatttcc cggctttgcc
     5161 tgcttctttg cggtgccagt ttgagcgtct gcggaccagg cgcaagcggc gcggtccggc
     5221 ccgccttagg atgtggtcag aaacaacttc ccctatttgc accggcggat ttgggcgcac
     5281 ag
//
LOCUS       DAOSLL010000309         5274 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_13996_length_5274_cov_3.867791, whole genome shotgun sequence.
ACCESSION   DAOSLL010000309 DAOSLL010000000
VERSION     DAOSLL010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5274)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5274
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13996_length_5274_cov_3.867791"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(442..1308)
                     /locus_tag="PLB25_13285"
     CDS             complement(442..1308)
                     /locus_tag="PLB25_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE42589.1"
                     /translation="MKPIYREFETQAQLDAQYNPALNLADISAPGRHYAQQSAQARAR
                     LRGVLDVPYGATLAETLDIFPADLPHAPVFVFLHGGYWRALSARDFSCIALGLQPLGI
                     TTVVVNYALCPLVTLDEITRQVRAAVAWTYRNIAGFGGDPARIALGGHSAGAHLTAMC
                     LQTPWAQDYGLPQDPLAAALLVSGIYDIEPLRYSFLQPQIRLDDGIISRNCPAFSVRR
                     CSTPIWVNWGGNETPEFARQARLFHAAWLAAGNSAEISTLPACDHYQVIHGFENPQHA
                     LCQWLARELGTK"
     gene            complement(1312..2022)
                     /locus_tag="PLB25_13290"
     CDS             complement(1312..2022)
                     /locus_tag="PLB25_13290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE42590.1"
                     /translation="MTALLEIDHLQSAYGASQVLFDINLCIQAGQVVTLLGRNGMGKT
                     TTVRSILGLTRASGGTVRFRGERIDQLRPDRIARMGLAVVPEGRQIFPNLSVKENLLA
                     FAANRNGTSQPWTLERVFALFPALAGRSGNMGNQLSGGEQQMLAIGRALMTNPHLLIL
                     DEATEGLAPLVREEIWRCLDQLRAQGQTILVIDKYVHRLIKLADHHTIIERGTVVWQG
                     DSAQLAADPGLWQRYIGV"
     gene            complement(2019..2783)
                     /locus_tag="PLB25_13295"
     CDS             complement(2019..2783)
                     /locus_tag="PLB25_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE42591.1"
                     /translation="MTHSTLLQADGLVKRFGGLVATDHAHVAVLAGEIHALIGPNGAG
                     KTTLIHLISGALTASAGRVLFDGMDVTRMPMPARVSHGLARSYQITSIFKRLSVLDNV
                     ALAVQAHAGNSFDVWQPARLDAERAAMAAEVVQRVGLAAQMHQIAGALSHGEQRQLEV
                     GLALATRPKLLLLDEPMAGMGPDESERMLALLQSLRGETSMLLVEHDMNAVFRLADRI
                     SVLVYGRVIACGTPDDIRADPQVRLAYLGDDAEAAL"
     gene            complement(2780..3844)
                     /locus_tag="PLB25_13300"
     CDS             complement(2780..3844)
                     /locus_tag="PLB25_13300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOE42592.1"
                     /translation="MGSKLAQVPATGPEAAPVPVARFLPGLFDHPLSALPGLGLLIGL
                     LLLPLLALALKQDFYIGVASRILIFALAASSLNLILGFGGMVSFGHAAFVGVGAYAVG
                     ILMQHGFTSAWLSWPVAFVASGLFALLVGAISLRTRGVYFIMITLAFAQMLYYLMISL
                     KTYGGFDGLSLSGRSVVGFGLDLSGDSTFYYVVLALAVLVFLALQRLLNSQFGHALQA
                     IRENEPRMAAIGFSVYRFKLLAFTLAGSLAGLAGALLANQGGFVSPSLMQWSQSGMLM
                     VMVILGGVGHLYGGLVGAVVFLVLEEVLSTYTIHWQLGLGAGLLAVVLLAPNGLLSLR
                     RQKPPKASPAGPVDAGKSPA"
     gene            complement(3837..4754)
                     /locus_tag="PLB25_13305"
     CDS             complement(3837..4754)
                     /locus_tag="PLB25_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HOE42593.1"
                     /translation="MGILILEQSLNGLQFGLMLFLLAAGLTLVFGIMDMINLAHGSIY
                     MLGAYLVASIASASGSFWLGLGAGVLATAAIGVLLELTVLRRLYQRDHLSQVLGTFAI
                     LLMSNEAVRMVWGSQPIALNPPAALAGPVELLPGFTYPAYRLFIIGVGLAVALLLYLL
                     VTRTRAGMQVRAGASNREMAQAMGVDVQWLFTAVFAVGAALCAVAGGLLGPLLAVQVG
                     MGESILILAFVVIVIGGIGSIRGALLGAMLVGVVDTAGRTLIPLLVDRLAGADVAANA
                     GPALASILIYVLMAAVLFFKPQGLFPAHG"
     gene            complement(4766..>5274)
                     /locus_tag="PLB25_13310"
     CDS             complement(4766..>5274)
                     /locus_tag="PLB25_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE42594.1"
                     /translation="GLGVNFIKQFVGAGLSKDMTLFGPGFSADEDVIRAVGEPMLGMF
                     NSSQWAHDMDNPVNKRFVADFQKDFGRLPTLYAAQGYDAARLMDAAVRDAKGKLDDKE
                     AVRKALKAAKFDSVRGAFRFNTNQYPIQDYYLRLITKDAQGRITNRTVGTVFKNHSDA
                     YASECKMP"
BASE COUNT         1163 a         1709 c         1543 g          859 t
ORIGIN      
        1 ggctctgaaa cccgtggttg gcctggaatt gatcacagcc aggcaaagtt cgcaatgccc
       61 aaaaatgcta tattgtttat agcttccagc gcatatacat cgggggctgg aagcctattt
      121 tgtgcctagt tttcgggcca gccactggcg cagtgcgtgc tgcgggctct gaaacccgtg
      181 gttggcctgg aattggtcac agccaggcaa ggttcgcaac gcccagaaat gctatattgt
      241 ttatagcttc cagcgcatat acatcggggg ctagaagcct attttgtgcc tagttttcgg
      301 gccaaccact ggcacagcgc gtgctgcagg ctctgaaacc cgtggttggc ctggaattga
      361 tcacagccag gcaaagttcg caaccccagc aacgctatat tgtttatagc ttccagcgca
      421 tatacatcgg gggctagaag tctattttgt gcctaattct cgggccaacc actggcacag
      481 cgcgtgctgc gggttctcaa acccgtggat gacctggtaa tgatcacaag cgggcaaggt
      541 gctgatttct gcgctgttgc ctgcggccag ccaggcagca tgaaacaggc gcgcctgacg
      601 ggcaaactcg ggcgtttcgt tgccacccca gttgacccag atcggcgtgc tgcagcgccg
      661 caccgagaaa gccgggcaat tgcggctgat gatgccgtcg tcaagcctga tctgtggctg
      721 caggaagctg tagcgaagcg gttcaatgtc gtaaatgccg ctgaccagca gcgctgccgc
      781 cagcgggtct tgcggcaaac catagtcttg tgcccatggt gtttgcaggc acatggccgt
      841 caggtgcgca ccggcagagt ggccacccag cgcgatgcgg gccgggtccc cgccgaaacc
      901 ggcaatgttg cgataagtcc aggccaccgc agcgcgcacc tggcgggtga tttcatccaa
      961 tgtgaccaat gggcacagag cgtaattcac caccaccgtg gtgatgccca gcggctgcag
     1021 gccaagggcg atgcaactga agtcccgagc ggacagcgcc cgccaatagc ccccatgcag
     1081 aaaaacaaac accggcgcat gcggcaggtc ggccggaaaa atgtcgagcg tctcagccaa
     1141 cgtggcgcca tagggcacat ctagcacacc acgcaaacgc gcgcgggcct gcgcggactg
     1201 ctgcgcgtaa tgtctgccag gggcgctgat gtcagccaga ttgagcgccg ggttgtactg
     1261 ggcatcaagc tgcgcctggg tctcaaattc gcggtaaatc ggtttcatgc ttcagacacc
     1321 gatatagcgc tgccacaagc cggggtcagc cgcgagttgg gcagaatcac cctgccatac
     1381 caccgtgccg cgctcaatga tggtgtggtg atcagccagc ttgatcagcc gatgcacgta
     1441 tttgtcaatc accagaatcg tctgcccctg cgcccgcaac tggtccagac aacgccagat
     1501 ttcctcgcgc accagcggcg ccaggccctc ggtggcttca tcgaggatca gcaaatgcgg
     1561 gttggtcatc agggcgcggc caatcgccag catttgctgc tcgccgccag agagctggtt
     1621 acccatgttg ccgctgcgac cagccagagc gggaaacaag gcaaacacgc gctccagcgt
     1681 ccagggctgg ctggtgccat tgcggttggc ggcaaaggcc agcaggtttt cttttactga
     1741 caggttggga aagatctgcc gcccttcggg caccaccgcc agccccatgc gggcgatgcg
     1801 gtcgggcctg agctggtcga tgcgttcgcc gcgaaaacgc accgtgccgc cgctggcgcg
     1861 ggtcaacccc agaatcgaac gcaccgtggt ggttttgccc atgccgttgc gccctagcag
     1921 ggtgaccacc tggcccgctt gaatgcacaa attgatgtca aacagcacct ggctggcacc
     1981 gtaggcgctc tgcagatggt caatttccag cagcgcagtc ataacgcagc ctcggcatca
     2041 tcacccagat aggccaggcg cacctgcggg tcggcgcgga tgtcatcggg ggtgccgcag
     2101 gcaatcaccc ggccatagac cagcactgaa atgcggtcgg ccagccggaa caccgcattc
     2161 atgtcgtgct ccaccagcaa catgctggtt tcgccgcgca ggctttgcag cagcgcaagc
     2221 atgcgctcgg actcgtctgg ccccatgccg gccatcggct catcgagcag cagcagcttg
     2281 ggccgggtcg ccagcgccag gccaacttca agctggcgct gttcgccatg cgacagggca
     2341 ccggcaatct ggtgcatttg cgcggccaag ccaacccgct gcaccacctc ggcggccatc
     2401 gcagcccgct cggcatcgag ccgcgctggc tgccaaacat caaagctgtt gccagcgtgg
     2461 gcctgcactg ccagcgccac gttgtcgagc accgacaggc gtttgaaaat gctggtgatc
     2521 tgatatgagc gcgccaaccc atggctgacc cgcgcgggca tcggcatgcg agtgacatcc
     2581 atgccgtcga aaagaacccg tccggcgctg gcagtcaggg caccggaaat caggtggatc
     2641 agcgtggtct tgccagcccc atttggccca atcagcgcgt ggatttcacc ggcgagcacc
     2701 gcaacatggg catgatcggt ggcaacaagg ccgccaaaac gcttgaccag gccatcggcc
     2761 tgcagcaggg tgctgtgcgt catgccggag actttccagc atcaaccggt ccggctggtg
     2821 atgccttcgg tggcttttgc cgacgcaaac tcagcaaacc gttgggtgcc agcagcacca
     2881 ccgccagcag cccggcaccc aggccgagtt gccagtggat ggtgtaagtg ctcagcactt
     2941 cttccagcac caaaaacacc accgcgccga ccaatccacc atacaaatgg ccgacaccgc
     3001 ccagaatcac catcaccatc agcatgcccg actggctcca ctgcatcagc gacggactga
     3061 cgaacccgcc ctggttggcc agcagggcac ccgccagccc ggccaggctg ccagccagcg
     3121 tgaaggccag cagcttgaag cggtaaaccg aaaacccgat ggccgccatg cgtggctcgt
     3181 tttcccgaat agcttgcagc gcgtgaccaa actgcgagtt cagcagccgc tgcagcgcca
     3241 gaaacaccag cacggcgagc gccagcacca catagtaaaa agtgctgtcg ccgctcaagt
     3301 ccagcccgaa accgacaacc gagcgccccg acagcgacag gccatcaaag ccaccatagg
     3361 ttttcagcga gatcatcagg taatacagca tctgcgcaaa agccagcgtg atcatgatga
     3421 agtacacgcc acgggtgcgc aggctgatgg cacccaccag cagcgcaaac agcccgcttg
     3481 cgacaaaggc gaccggccag ctcagccagg cggaggtgaa gccatgctgc atcaaaatgc
     3541 ccaccgcata ggccccgaca ccgacaaacg ccgcatggcc aaaactgacc atgccgccaa
     3601 aacccagaat caggttcagg ctgctggccg ccagcgcaaa gatcagaatg cggctggcca
     3661 cgccgatgta aaaatcttgc ttgagtgcca gcgccagcaa aggcaacagc agcagaccga
     3721 tcagcaaacc caggccgggc aacgccgaga gcgggtggtc aaagaggccc ggcaagaacc
     3781 gcgcaaccgg caccggcgcg gcctcgggtc cggtcgcagg gacttgcgcc aacttgctac
     3841 ccatgcgccg gaaataagcc ttgcggcttg aaaaacagca cggcggccat caacacataa
     3901 atcaggatcg aggccagcgc cggtccggcg ttggcggcca catcggcacc ggccagccgg
     3961 tcaaccagca aggggatcag cgtgcgcccg gcggtatcga ccacgccgac cagcatggca
     4021 cccagcaagg cgccgcgaat cgaaccaatg ccgccaatca cgatcaccac aaaggccagg
     4081 atcaaaatgc tctcgcccat gccgacctgc accgccagca gcggcccgag cagaccaccg
     4141 gccaccgcgc acaacgcggc cccaacggca aacacggcgg taaacagcca ctgcacatca
     4201 acccccatgg cctgcgccat ttcgcggttg gatgcgccgg cacgcacctg catgccggcc
     4261 cgggtgcggg tgaccagcag gtaaagcagc agcgccaccg ccaggcccac gccaatgatg
     4321 aacaagcggt aagccgggta ggtgaagccg ggcaacagtt ccacaggacc ggccagcgcg
     4381 gcaggcgggt tcagggcaat cggttgtgaa ccccagacca tgcgcaccgc ctcgttgctc
     4441 atcagcaaaa tggcaaaggt gcccaatacc tgcgacaaat ggtcacgctg gtacagacgg
     4501 cgcaacacgg tgagttccag cagcacgcca attgccgccg tagccagcac accagcaccg
     4561 agacccagcc agaatgaacc gctggcactg gcaatgctgg caaccaaata ggcacccagc
     4621 atgtagatgg agccatgcgc caggttgatc atgtccatga tgccaaacac cagcgtcagc
     4681 ccggccgcca gcaagaacag catcaggcca aactgcaagc cgttgagcga ctgctccagt
     4741 atcaaaatgc ccatgcgtca ggcattcatg gcatcttgca ttcgctggcg taggcatccg
     4801 aatggttctt gaacaccgtg cccaccgtgc ggttggtgat gcggccttgc gcgtccttgg
     4861 tgatcaggcg caggtagtag tcctgaatcg ggtattggtt ggtgttgaac ctgaaggccc
     4921 cgcgcaccga gtcgaacttg gccgctttca aagccttgcg cacggcctcc ttgtcatcga
     4981 gcttaccctt ggcatcgcgc accgccgcgt ccatcaggcg cgcggcgtcg taaccctggg
     5041 ccgcgtacag ggtcggcagc cgaccaaagt ccttctgaaa atcggccaca aagcgcttgt
     5101 tgaccgggtt gtccatgtcg tgcgcccact ggctgctgtt gaacatgccg agcatcggct
     5161 cgccgaccgc gcggatcacg tcttcgtcgg ctgaaaagcc ggggccaaac agcgtcatgt
     5221 ccttggacaa accggcaccg acaaactgct tgatgaagtt gacccccaga ccac
//
LOCUS       DAOSLL010000310         5261 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14029_length_5261_cov_4.589128, whole genome shotgun sequence.
ACCESSION   DAOSLL010000310 DAOSLL010000000
VERSION     DAOSLL010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5261)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14029_length_5261_cov_4.589128"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="PLB25_13315"
     CDS             complement(<1..479)
                     /locus_tag="PLB25_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOE42595.1"
                     /translation="MPPDFWSTAPLLAGAAFAAGVLNAIAGGGSFLTLPALVFTGVPP
                     IMANATSALAVSPGYLGSTLGFRAELRCLPLPRLKREMVISAVGGIVGALLLLVTPAK
                     VFSSIVPWLLLFATVLFAAGPAIARRAAGASGSSHALTRWREPGLALVAIYGGYFNGG
                     "
     gene            1029..1745
                     /locus_tag="PLB25_13320"
     CDS             1029..1745
                     /locus_tag="PLB25_13320"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE42596.1"
                     /translation="MYRLFFEVQKKHWWFVSRKEIVLSVIDRHMNRNAHARVLDIGCG
                     SGVMLTALGDLGDIYGMDMSDDAISFSKEIFSGKVEKGFLPDHVPYEKNFFDLITALD
                     VIEHIDQDVESLKAIHSRLVSDGKAIITVPAYMLLWSKFDELNEHKRRYTLGELKKKL
                     VQAGFTIEKITYYNTLLFPVIFMVRMLNNIFQRDGKSDIDLPNLKLNSVLGKIFGAEK
                     YLLKFFNMPFGLSILAVVKK"
     gene            1742..2770
                     /locus_tag="PLB25_13325"
     CDS             1742..2770
                     /locus_tag="PLB25_13325"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE42597.1"
                     /translation="MTSIQGALKAEASKAINSLPLQQVSIVIPVYQGEKTLPTLIEEI
                     VPFTLSPLTPDGIKYVICEVILVHDCGSDRSDSVMERLAAEYSFVKTIWLSRNFGQHA
                     ATMAGMANARGDWVATIDEDCQQNPADIGRMLDTAVRASLQLVYAQPTNPPPHGWVRN
                     ALSRAAKSIIPKMLGRGQLGKFNSFRLIDGDIARTMAAYCGNGVYLDVGLFWIVGRVG
                     HCPLQLRNELDRPSGYSYFKLLNHFWNLILTTGTHPLRLITLMGFNSVLLAVGISGYA
                     LYGKFVNNDPVQGWASLLIVVSFFSGATLTSLGVIAEYLAVTMGIVMGKPLYVISTKP
                     TRPEIDDN"
     gene            2872..3288
                     /locus_tag="PLB25_13330"
     CDS             2872..3288
                     /locus_tag="PLB25_13330"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HOE42598.1"
                     /translation="MKRKHLGVLWREIVYLTRHAGAGIVNTLVGFAVIFSAMRLGLSP
                     LLSNVAGYAVGFVLGFVISKKFVFRSKGHLITESILYLVAFAISFGFNLLVLQVALMY
                     FNLGVIASQIVAAVAFTMLMYLLSRLLVYRALKSVH"
     gene            3778..>5261
                     /locus_tag="PLB25_13335"
     CDS             3778..>5261
                     /locus_tag="PLB25_13335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE42599.1"
                     /translation="MPPSSASTPAKPLSGPQKALQKLGLIRPIDLALHLPLRYEDETR
                     LVQLRDLREGDVAQVEGTVRHCEVKLGGTMQLLVTLDDGTGTCLLRFFTIYPSMQKAL
                     AVGKQIRVRGEVRGGFAGLTMMHPAYQSAGGELPSALTPVYPTVAGLPQAYLSKAVQA
                     GLERADLTDTLPAEFLNEIGLQPLWNLREALLFLHHPGPETTLATLTERSHPAWQRLK
                     AEELLAQQISQLQAKRARAARLAPVLMPGFDLAALVDKKAALDQRTSPIASAPEAGRI
                     SGIDSQNRPQPGVPDLPVDGPVVAQRAGSKWAAAGVAQPLHDRSDRLLAPLHQRLLAA
                     LPFELTAAQHRVCAEIIGDLRRPVPMHRLLQGDVGSGKTVVAALAAVVCIEAGWQCAL
                     MAPTEILATQHFAKLVSWLQPLGITTAWLTGSQKARERRDMLALIASGQAQLVVGTHA
                     IIGEKVQFQKLALAIIDEQHRFGVAQRLALRGKLTDAASAPPDD"
BASE COUNT         1192 a         1348 c         1463 g         1258 t
ORIGIN      
        1 ccaccattga agtaaccacc gtaaatggcc accagtgcca ggcccggttc acgccaccgg
       61 gtcagcgcat ggctgctgcc cgaagccccc gctgcacgcc gcgcaatggc cggaccggct
      121 gcgaacagca cggtggcaaa cagcagcagc cagggcacga tactggaaaa gaccttggcc
      181 ggggtgacca gcaacagcag cgcgccgacg atgccgccaa cagccgaaat gaccatttcg
      241 cgcttcagcc ggggcagtgg caggcaacgc agctcagccc gaaaacccag ggtgctaccc
      301 aggtagcctg ggctcaccgc cagcgcgctg gtggcgttgg ccatgatcgg cggcacacca
      361 gtgaaaacca gcgcgggcag ggtcaaaaaa ctgccaccac cggcaattgc gttgagcacg
      421 ccagcggcaa acgcggcacc agccagcagc ggagcagtgg accagaaatc aggtggcatg
      481 cagatattgt cgcagggccg cagccgatct gttggcatgc accagaccgc ccggccatgt
      541 ttcatgccaa gcccaatcac ttgaattgac ccggccgcta ctacaagtgc tccacaccgt
      601 cagcccgact gcctgcgctg cgccaattga ctgtcgggct gggcaatcga gcagcgggaa
      661 atggcaacag cgatgggtca aaaggcccga cacaaaatgg ccgctgcagt cggttcgcag
      721 ccctgctggc gaagttgtgt tgtcgcacag gaggtcggag atacacaaag acaggcggcc
      781 ttctgctgat tggtctgaat ggcttgacgg gcgctacgac catattgctc ggctgcttgc
      841 caacaaatag ccgtggctgc attcgcatgt cctgaccggg ccatttggcc gctcccctgc
      901 caaagcaaat gcgcatgcac caagggatca agagtttttt atagagggtc gagcgatgtt
      961 tcaaggtacc atactggcgc gcatgtggtt aacaagccaa gctaacatag gttacgatga
     1021 atatagaaat gtaccggtta ttttttgagg ttcaaaaaaa acattggtgg tttgtcagca
     1081 ggaaagaaat tgtactgagc gttatcgaca ggcatatgaa caggaatgct catgccagag
     1141 tattggacat cggttgcggc agtggcgtta tgctgacagc actcggagat ttgggtgata
     1201 tctatggcat ggacatgtca gatgatgcca ttagtttcag taaagaaata ttcagtggca
     1261 aagtcgaaaa gggttttctg ccagatcatg tgccttatga aaagaacttc tttgatctga
     1321 ttaccgctct ggatgtgatt gaacacatag accaagatgt cgaatcgttg aaggcgatac
     1381 attcgcgtct ggtttcagat ggcaaggcaa tcattaccgt acccgcttat atgctactgt
     1441 ggtcaaagtt tgatgaactg aacgaacaca agcgaagata tacgttggga gagttgaaaa
     1501 aaaaactggt gcaagcaggt tttacaatag aaaaaataac ctattataat accttgctat
     1561 ttcctgttat tttcatggtt cgcatgctga ataatatttt ccaaagagac ggcaaatctg
     1621 atattgattt gcctaacttg aagttgaatt cagttcttgg gaaaatattt ggtgcagaaa
     1681 agtatttatt gaagttcttc aatatgccat ttggcttatc aattctggcc gtagtcaaaa
     1741 aatgacatca attcaaggtg cgctgaaagc agaggcttct aaagcaataa acagtttacc
     1801 gcttcaacaa gtctctattg tcatacccgt ttatcagggc gaaaaaacac ttccgacttt
     1861 aattgaagag attgtgccat ttactttgtc tccgttgaca cctgacggaa ttaagtatgt
     1921 aatctgcgag gtaatattgg tacatgattg cggatctgac cgttctgatt ccgtcatgga
     1981 aagactcgct gcagaatatt catttgtcaa aaccatttgg ctgtcgcgaa atttcgggca
     2041 acatgccgcg acgatggcag gcatggcaaa cgcacgtggc gactgggttg ccaccattga
     2101 cgaagattgt cagcaaaacc ctgcggatat tgggcgcatg ctcgataccg ctgtccgtgc
     2161 atcactgcaa ctcgtttacg cccaacccac caatccaccg ccccatggct gggtgcgcaa
     2221 tgctttgagt cgagctgcca aatcaatcat tcccaaaatg ctcggccgtg gtcagctagg
     2281 gaagttcaat agtttccgac tgattgatgg cgatatcgcg agaacaatgg cagcttactg
     2341 tggcaacggt gtctatctcg atgttggcct cttttggatt gttggccgtg ttggtcactg
     2401 ccctttgcaa ctgagaaatg aactcgaccg accttcgggg tactcatatt tcaagctgtt
     2461 aaatcatttc tggaacttga ttctgacgac agggacccat ccgctcagac tgattactct
     2521 gatgggtttt aactctgtat tattggcggt gggaatatca ggttacgcac tttacggaaa
     2581 atttgtgaac aacgatccgg tacaaggttg ggcatcgcta ttaattgtgg tctctttctt
     2641 ttctggcgcg acgttgacct cattgggtgt gattgcggag tatcttgcgg taaccatggg
     2701 gattgtgatg ggtaagccct tgtatgtcat tagcacaaaa cctacgcggc ctgaaatcga
     2761 cgacaactga ttgaatggtg tctgtgaaac tgtcactttg tgtgtgccaa agcttgatgt
     2821 attgaccttg cacccagacg gtaatctaac tattttaagg gtagccgacc agtgaagcgt
     2881 aaacatttag gcgtcttgtg gcgggaaatc gtgtatctga ctcgccatgc tggcgctggc
     2941 attgtgaata cattggttgg atttgccgtt atattttcag caatgcgctt ggggctgtcg
     3001 cccttgcttt ccaatgtcgc tgggtatgcc gtgggatttg ttctgggatt tgtgatttca
     3061 aaaaaatttg ttttcaggag caaagggcac ttgataacag aaagcattct gtatctcgtc
     3121 gcttttgcta tttcatttgg tttcaattta ttagtgttgc aagtcgcgtt aatgtatttc
     3181 aaccttggtg ttatcgcatc acaaatcgta gctgccgtgg cttttacaat gcttatgtac
     3241 ttattgtctc gcttgcttgt gtacagagcc ttaaaatcag ttcattgagc atgtaaattt
     3301 tgcaaactga ttctttttga taaagggatt caagttaact cagactgcat cctggtttgc
     3361 ttgtttgttt ttttgtcagc atcctcgcgc agggccaagt catttttcag ggacaggctg
     3421 tcgatatttg tgcagttttg cccctttggc ttttgatgct ttgatacttg ttgcttgaca
     3481 gtggcgttga tccgcaccca cacgccccaa tcgctggttc cccgatgcga gcggggccgg
     3541 gccatggtcg gctggtgagc aatggtgcaa cctttgggtt cccccttgcg ggcggtgacc
     3601 ggcccatgta tctggtggct gcccagggtg atgcacaggg ttcagcgcca gcttcactgc
     3661 cgcgccagtc aaaatggcag ccgagcattg ggcaagcgct ttttgtacca aattgacctc
     3721 tagcccttat gcaagaagcg ccaccagcta tcatttttat ctgtgcaaaa tcagcgcatg
     3781 ccgccgtctt ccgccagtac tcctgccaaa cccttgtctg ggccgcaaaa ggccctgcaa
     3841 aagctcggct tgattcggcc aattgatctg gccttgcatc tgccgctgcg ctacgaggat
     3901 gaaacccggc ttgttcaatt gcgcgacctg cgtgagggtg atgtggcgca agtggaaggc
     3961 accgtcaggc actgcgaggt caaacttggc ggcacgatgc agttgctggt cacgctcgac
     4021 gacggcactg gtacctgcct gctgcgcttt tttaccattt acccgtcgat gcaaaaggcg
     4081 ctggcggtgg gcaagcagat tcgggtgcgc ggtgaagttc ggggtggttt tgccggcttg
     4141 acgatgatgc atccggcgta ccagagcgcc ggtggcgaac tgcccagcgc cctgacgccg
     4201 gtttacccga cggtagccgg tttgccgcag gcctatctga gcaaggcagt gcaagccggt
     4261 ctggagcggg ccgacctgac agacaccctg ccagccgaat ttttgaatga aatcggcctg
     4321 cagcccttat ggaacttgcg tgaggcgcta ctttttttgc accaccctgg gcctgagaca
     4381 acgctggcga cactgactga gcgcagtcac ccggcctggc agcggctgaa ggcggaagag
     4441 ttgctggcgc agcaaatctc gcaactgcag gccaaacgcg cacgggctgc ccggctagcc
     4501 ccggtgctga tgcctggctt tgacctagcc gcattggttg acaaaaaggc cgccctggac
     4561 cagcgcacct cacccatcgc cagcgcgcca gaagccggtc ggatttctgg catcgacagc
     4621 cagaatcgac cacagccagg ggtcccggat ttgccagtcg atggcccagt cgtagcgcaa
     4681 agggccggtt caaaatgggc cgcagcaggc gtggcgcagc cgctgcacga taggtcagac
     4741 aggctgcttg cgcctttgca tcagcgcttg ctggccgcgc tgccgtttga gcttaccgcg
     4801 gcccagcacc gggtctgcgc cgagatcatt ggcgacctgc gtcgcccggt tcccatgcac
     4861 cggctgctgc aaggcgatgt gggttccggc aaaacggtgg tggcggcatt ggccgcagtg
     4921 gtgtgcattg aggcgggctg gcagtgcgcg ctgatggcgc caaccgaaat tctggcgacc
     4981 cagcactttg ccaagctggt gagctggctg cagccgctgg gcatcaccac cgcctggctc
     5041 accggcagcc agaaggcaag ggaacggcgc gacatgctgg cgctgattgc cagcggtcag
     5101 gctcaactgg tggtgggcac ccacgccatc attggcgaga aggtgcagtt ccagaagctg
     5161 gcgctggcca tcattgacga gcaacaccgc tttggcgtgg cacagcggct ggcgctgcgc
     5221 ggcaagctga ctgatgctgc cagtgccccg ccagatgatg a
//
LOCUS       DAOSLL010000311         5249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14073_length_5249_cov_4.225645, whole genome shotgun sequence.
ACCESSION   DAOSLL010000311 DAOSLL010000000
VERSION     DAOSLL010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5249
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14073_length_5249_cov_4.225645"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="PLB25_13340"
     CDS             <1..538
                     /locus_tag="PLB25_13340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HOE42600.1"
                     /translation="EGCQMDLTQTRYLDYPGLQSYCHLVAGVVGEVAAQIFGQTKPQT
                     TSYAHTLGQALQLTNIIRDVGEDAMRGRVYLPQTELRQFGVTVQELLNRSYSTRFTAL
                     MAFQAERAHGLYDQALALLPATDRYAQKPGLMMASIYRALLTEIEQDGFAVLHQRISL
                     TPLRKFWLAWKVQALGRL"
     gene            711..2051
                     /gene="hpnE"
                     /locus_tag="PLB25_13345"
     CDS             711..2051
                     /gene="hpnE"
                     /locus_tag="PLB25_13345"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HOE42601.1"
                     /translation="MNIAIVGAGWAGMAAAVTATQAGHHATVFEASSAVGGRARGLKA
                     SLPDGTPVLLDNGQHILIGAYTETLRLLRLVAVEPASALLNQPMALQFPDGAGIRFPD
                     WPTPLDALAGIVGARGWSLADKWALLRAAASWQLSGFVCPPDLSVTSLCQPLTARVKA
                     ELIEPLCVSALNTPADQASASVFLRVLRDALFGVAGGSHLLLPRVDLGALFPEAAVNW
                     IRQLGGVVHLKARVDSVSRVGRQWQINGPALDQQASQRFAAVILATSASNAALALTQS
                     ALAATNIISIGWLDWAALARSLAFEAIATVYAWAPAAALPRPMLALRCNAASPAQFVF
                     DRAQLGGPAGLLAFVVSAAQGERQALQARVLAQAHSQLGLTLQAVQTVVEKRATFACT
                     PGLVRPPAQIAPGLFACGDYVAGPYPATLEGAVISGVQAVQRIAGSSLAKSAPL"
     gene            2088..4511
                     /locus_tag="PLB25_13350"
     CDS             2088..4511
                     /locus_tag="PLB25_13350"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HOE42602.1"
                     /translation="MKLALKILVALLLVALLAAAGGGLWYVLGKLPQRSGDLTLPGLT
                     APVSVRYDDRGIPHIKAGNEPDLYRALGFVQAHDRLFQMEMARRLARGELAEILGPKL
                     LKLDRMFRTLGLGEHADKTVAKMDLQSPAVLAQLAYLDGINQFQASQPAPMEFDLTGI
                     AKRPFTLQDTVAVSGYLAYSFAAAFKTEPAMSFIQSELGDAYLTIFDLEWNPQGVLSP
                     APGAARKPDWQALTQLAQVSGQAQHLSGVALFEGSNAWAVSGQRTSSGKPMLAGDPHI
                     GFSLPSVWYEAHLSAPGFELYGHFQALNAAALLGHNQQFGWSLTMFQNDDIDLIAETV
                     NPQQPTQVKFHDQWVELETRQEIIKVKGGKPVTLNLQRSPHGPIISNAFRDTLGDAPV
                     AMWWAWLETDNPLLEAFYQLNRADTLAKARAAAGNIHAPGLNLVWASASGDIGWWAAA
                     KLPIRPPGVNPAFILDGATAAADKLGFYRFSDNPQEENPRRGYVLSANQQPKSTSGLP
                     VPGYYNPPDRAQALEDRLGNNQIQWNALNNQSLQLSTQTGYFWRVLEPLLPVLSEVVR
                     DPLERSVFDSLTMWDGQYTAFNIPPTVFTQFLYELARAAMADELGDVQFKNLLGTRAL
                     DLALPRLAADEESLWWDNVKTEKVETRKDIMRIAWRATMAHLKATLGKSPNDWGWGQA
                     HTLTHVHPLATPMRLGWLLNVGPFDVPGGREVPNNLANPIGPAPWAVTYGPSTRRVID
                     FADAAQARGINPVGQSGVPFDRHYQDQAAAFAVGGYMQQFLSEKDVAPNTRSTLTLKP
                     GTGASATSK"
     gene            complement(5038..5249)
                     /locus_tag="PLB25_13355"
                     /pseudo
     CDS             complement(5038..5249)
                     /locus_tag="PLB25_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078828.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
BASE COUNT         1034 a         1671 c         1599 g          945 t
ORIGIN      
        1 cgaaggttgc cagatggacc tgacgcaaac ccgttacctg gattaccctg ggctgcagag
       61 ctattgccac ctggtcgccg gggtcgtggg tgaggtggca gcacaaattt ttggccagac
      121 caagccacag accaccagct acgcccacac gctggggcag gcgctgcagc tcaccaacat
      181 cattcgcgat gtcggcgaag atgccatgcg cggacgcgtt tacctgccgc agactgaact
      241 caggcagttt ggcgtgaccg tacaagaact gctcaaccgc agctattcga cgcgttttac
      301 cgccctgatg gcttttcagg cagagcgcgc ccatgggctt tacgaccagg cactggcact
      361 gctgccagcc actgaccggt acgcccaaaa gccgggcctg atgatggcca gcatctaccg
      421 tgccctgctg accgaaatcg agcaggatgg ctttgccgtg ctgcaccagc gcatcagcct
      481 gaccccgctg cgcaagttct ggctggcctg gaaggtgcag gcgctgggcc ggttatgacc
      541 aatgtcgccg tggtaaacct gatggcctgt cgaatttggc tcacgcgggc aacccacggc
      601 ccagcgggca tcggcaagag actggtgatc tgctggcaat gctgccgctc aaattacttg
      661 tcaatcagct tggcagagct ggccaacacg tccggcagcg cggccagccg atgaatattg
      721 ccattgttgg cgctggctgg gccggcatgg cggcggctgt cacggccacc caggcgggcc
      781 atcatgctac tgtttttgaa gcatccagcg cagttggcgg aagggccagg ggcctgaaag
      841 catcattgcc tgacggcaca ccggtactgc tcgacaacgg ccagcacatc ctgattgggg
      901 cctacaccga aaccctgcga ctgctgcggc tggtggctgt ggagcccgcc agtgccttgt
      961 tgaaccagcc catggcactg caatttcccg atggcgcagg catccggttt cccgactggc
     1021 caactccgct ggacgcgctg gccggcattg ttggcgcacg cggctggtca ctggcagaca
     1081 aatgggcgct gctgcgcgcc gccgccagtt ggcagctcag cggttttgtc tgcccgccag
     1141 acttgagcgt gaccagcctt tgccagccgc tgacggcccg ggtcaaggcc gaactgatcg
     1201 agccgctgtg tgtgtcggcg ctgaacaccc cggctgacca ggccagcgcc agtgtttttt
     1261 tgcgggtgct gcgcgacgcg ctgtttggtg tggcgggtgg ctcgcacctg ctgctgccca
     1321 gagtcgatct gggggcactg tttccggaag ctgccgtcaa ctggatccgc cagctcggcg
     1381 gcgtggtgca tctgaaggcc cgggttgaca gcgtcagccg ggtcggtcgg caatggcaga
     1441 tcaacgggcc agcactggac cagcaggcaa gtcagcgctt tgccgcggtg attctcgcca
     1501 cttcagcatc aaatgcggct ctagccctta cccagtctgc gctggcagct acaaatatca
     1561 tatctattgg ctggcttgac tgggccgcat tggcacgctc gctggcattt gaggcgattg
     1621 ccacggtgta cgcctgggca cctgctgcgg ccctgccccg gccaatgctg gccttgcgct
     1681 gcaatgccgc cagcccggcg caatttgtct ttgaccgcgc ccaactgggt gggccagcgg
     1741 gcttgctggc gtttgtggtt agcgcagccc aaggtgagcg ccaggcactg caggcccgcg
     1801 tgctggcgca agcgcacagc caactcggcc tgacgctgca agcggtgcaa acagtggttg
     1861 aaaaacgcgc cactttcgcc tgcacgccgg gcctggtcag gccaccggcg caaattgcac
     1921 cggggttgtt tgcctgcggc gactatgtgg ctggccccta cccggccaca ctcgaaggtg
     1981 cggtgatcag cggcgtgcag gcagtccagc gcatcgccgg gtcgagcctg gcaaagtcag
     2041 cccctttatg atcaagctct aaccgagcca acgcgccctg ccctgccatg aaactagccc
     2101 tgaaaattct cgttgcactg ttgctggttg cgctgctggc tgcggcaggc ggcgggcttt
     2161 ggtacgtcct gggcaaactg ccacagcgct cgggcgacct gacattgcct ggcctgacgg
     2221 ccccggtcag cgtgcgctat gacgaccgtg gcatacccca catcaaagcg ggcaacgagc
     2281 ccgacctgta ccgtgcactg ggttttgtac aggcgcatga ccggctgttt caaatggaaa
     2341 tggcgcgtcg gctggcgcgc ggcgaactgg ccgaaatatt gggacccaag ctgctcaagc
     2401 tggaccgcat gtttcgcacc ctggggctgg gcgagcacgc cgacaagacc gtggccaaaa
     2461 tggacctgca aagcccggcg gttttggccc aattggctta cctggacggc atcaaccagt
     2521 ttcaggccag ccaaccggcg ccgatggagt ttgatctgac gggcatcgca aagcgcccct
     2581 tcaccctgca ggacacggtg gccgtgtcgg gctacctggc ctacagtttt gcggcagcct
     2641 tcaagaccga accagccatg agcttcatcc agagcgaact tggcgatgcg tatctgacta
     2701 tttttgacct ggaatggaac ccgcagggtg tcctcagccc ggcccccggc gccgcccgca
     2761 agcccgattg gcaggcactg acccagttgg cgcaagtcag cggccaggcc cagcacctgt
     2821 ccggtgtagc gctgtttgag ggcagcaatg cctgggccgt gtcagggcag cgcacctcaa
     2881 gcggcaagcc gatgctcgcg ggtgacccgc atatcggctt ttcgctgcca tcggtctggt
     2941 atgaggcgca cctgagtgcc ccagggtttg aactttacgg gcattttcag gcgctcaatg
     3001 ccgctgccct gctgggtcac aaccagcagt ttggctggag cctgacgatg tttcagaacg
     3061 acgacatcga cctgatcgcc gaaaccgtca atccgcagca gccaacccag gtcaagtttc
     3121 acgaccaatg ggtagaactg gaaacccgcc aggagatcat caaggtcaaa ggtggcaagc
     3181 cggtcacgct gaacctgcag cgctcgccgc acggcccgat catcagcaac gcctttcgcg
     3241 acacgctggg cgatgcgccg gtcgccatgt ggtgggcctg gctggaaacc gacaaccccc
     3301 tgctggaggc tttttaccag ctcaaccgcg ctgacacact ggccaaggcc agagctgcgg
     3361 cgggcaacat ccacgcacct ggcttgaacc tggtgtgggc cagcgccagc ggcgacatcg
     3421 gctggtgggc ggctgccaaa ttgccgattc gcccaccagg cgtgaacccg gctttcattc
     3481 tggacggcgc aacagccgcc gccgacaagc tgggttttta ccgtttcagc gataacccgc
     3541 aagaagaaaa cccgcggcgt ggctacgtct tgtcggctaa ccagcagcca aaatcgacca
     3601 gtggcctgcc ggtccctggc tactacaacc cgccggaccg ggcgcaagcg ctggaagacc
     3661 gcctgggcaa caaccagatc caatggaatg ccctcaacaa ccagtcgttg caactcagca
     3721 cccagaccgg ctacttctgg cgcgtgctcg aaccactgct gccagtgctc agcgaagtgg
     3781 tgcgcgaccc gctggagcgt tcggtgtttg acagcctgac gatgtgggac ggccaataca
     3841 ccgccttcaa cattccgccc accgttttca cccagttctt gtacgaactg gcccgtgccg
     3901 ccatggccga cgagcttggc gacgtgcagt tcaaaaacct gctcggcaca cgggcgctgg
     3961 acctggcctt gccgcggctg gcggcagacg aggaatcgct ctggtgggac aacgtcaaga
     4021 ccgaaaaggt ggaaacgcgc aaggacatca tgcgcattgc ctggcgcgcc accatggcac
     4081 acctgaaggc aacgctgggc aaaagcccga acgactgggg ttggggacag gcccacaccc
     4141 tgacccatgt gcatccgctg gcaacgccaa tgcggctggg ctggctgctc aatgtcgggc
     4201 cgtttgacgt gcctggcggt cgcgaagtgc ccaacaacct ggcaaacccg attggcccgg
     4261 ccccctgggc tgtcacttac ggtccatcga cccggcgcgt gattgacttt gccgatgccg
     4321 cccaggcgcg tggcatcaac ccggtcgggc aaagcggtgt gccgtttgac cgtcactacc
     4381 aggaccaggc tgccgccttc gcggtaggcg gctacatgca gcagtttctg agcgagaaag
     4441 acgtggctcc caacacccgc agcaccctga cgctcaaacc tggaacaggt gcgtcagcca
     4501 ccagcaagta gccttgtttg caaccaggca ccaaagtgtt gcaagccgcg acgaccagac
     4561 gcaagccaaa agccagcact ctggcgcgca ccctgcaagc gccaggcatg acaacaacga
     4621 catcgaggct ccagccaaca caaaacaccg ccagtgccct gtgtggttat tggaccagta
     4681 tcattttgat agctatcagc gcacaccgga catgggtctg atgtcaattt cttgcatatc
     4741 gcaggttcat tggcgaatcg acctggcaga ccgacgatga acctgacgca agacaccttt
     4801 ttcgatgccg tgcgccgttg cggcgaaata gctgtgcgaa caatggcagg agtctgccgg
     4861 actgacggcg cagcggctgc gacccgggcg cagcggcgca ttttgcgccg accttttggc
     4921 ccgcaacgag gccatggatc agcaaattcg gcatctctgg ctggggttga ttcacgttgc
     4981 ttgatccata agggatgcgg aaattgcaaa tatgccgctg atgacgagtg ctgacgggat
     5041 gcacagccag gcgctgatga acgaaaccac caacccaacc agaaacatcg gcgcatctgc
     5101 cagcgacaac aaggcgcgtt cttggtacag gctatagaca ccggcaccaa tcaaggtcac
     5161 aaatcgccag gtaagacgaa aaaatcggtt gccgccttgc gcgacaggcc cagcagcatg
     5221 ccgccaatgc tggtgggccc gctacggct
//
LOCUS       DAOSLL010000312         5247 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14084_length_5247_cov_5.244029, whole genome shotgun sequence.
ACCESSION   DAOSLL010000312 DAOSLL010000000
VERSION     DAOSLL010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14084_length_5247_cov_5.244029"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="PLB25_13360"
     CDS             complement(<1..268)
                     /locus_tag="PLB25_13360"
                     /inference="COORDINATES: protein motif:HMM:NF024886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HOE42603.1"
                     /translation="MNPVTKVEFDQRYQTHLKHLKLKGLQPKTIDAYSRAIRRMGDYF
                     DHAIDNLSPAQLTDYFSSLIGTYSWSAVKLDLYGFKFYTQHVLGK"
     gene            476..1423
                     /locus_tag="PLB25_13365"
     CDS             476..1423
                     /locus_tag="PLB25_13365"
                     /inference="COORDINATES: protein motif:HMM:NF022633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2971 domain-containing protein"
                     /protein_id="HOE42604.1"
                     /translation="MGTSLFKYVGPSNVARVFASKGFLTLKCGTPAEFNDPYELFLTM
                     NFRERPELIAFYTDVVGQLPQLPTTCFSRSPSVAPMWAHYARNLEGFVLELDESKVSA
                     ACPESEFGDVDYRDKPSSAVAENLYRAYEIGKFRYMHFLQRSVFSAAYYTKQRCWAYE
                     KERRMILDKTEVQVRNGLTLVDLPISTMQSLISGPRASDETVSALRAIAKDAGCRFWQ
                     TRIGRSTGVPYFVDDAGKPAVFKGAEIAPAKTYCASCTEPTSRRQAKLCSWCRIDDSH
                     RENAAQRNSYRILHHYGQLDSYLKSVNEIARRARRKNDA"
     gene            complement(1732..1974)
                     /locus_tag="PLB25_13370"
     CDS             complement(1732..1974)
                     /locus_tag="PLB25_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42605.1"
                     /translation="MVTLVKSERAAQRVMYSITLYLETTLKLKVNLVKSKVAPMSECA
                     FQSLSFGNASLSITLRIAATALPSKGASALSIASGG"
     gene            2081..2356
                     /locus_tag="PLB25_13375"
     CDS             2081..2356
                     /locus_tag="PLB25_13375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42606.1"
                     /translation="MSPPLQLLVELGQQDVCQQWREWPTLWCAHFAGFNVLSNQHPGA
                     QVAPIKRSSRLSAMERAGNFSSMSWLLVTQTQHQVATSPRLLPAGPR"
     gene            complement(2530..2730)
                     /locus_tag="PLB25_13380"
     CDS             complement(2530..2730)
                     /locus_tag="PLB25_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42607.1"
                     /translation="MAKLAHDGKPNLKLNLIKSKVAPMSECAFLGFTNKGKKVRVVDR
                     CRYLAASSGSQLRNANSQQKCL"
     gene            2956..3231
                     /locus_tag="PLB25_13385"
     CDS             2956..3231
                     /locus_tag="PLB25_13385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42608.1"
                     /translation="MKKASMKMADQDIPELKRDELCTGVPGKCLKHFLQGSNVVVLQP
                     EIQKAFPTSEAVNKALASMLAFAQEAQVGRRASGSQFSFDIGPNAFR"
     gene            3218..3619
                     /locus_tag="PLB25_13390"
     CDS             3218..3619
                     /locus_tag="PLB25_13390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42609.1"
                     /translation="MPSGDSTRVWFPEMLVELQKQWSRTLSWEDCGVLCERMTEARTK
                     LKLEKGLQGPMMHCPRCNAVHEMRLAPITIRSLLFALRKQGVLSDPELNQLDAAWKRY
                     RAKHRLDGCGKGREVPNMSPAATSACVQSLC"
     gene            complement(3981..4379)
                     /gene="rbsD"
                     /locus_tag="PLB25_13395"
     CDS             complement(3981..4379)
                     /gene="rbsD"
                     /locus_tag="PLB25_13395"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HOE42610.1"
                     /translation="MKRNLLLNAELSHLIATMGHGDMLVIGDAGLPIPQGPNAPQRID
                     LAVCPGTPRLADVLRAVMSELQVQSTVIATEALDAGGPPAWYTDQLNLTPRTVGHEEF
                     KKMCGAARAMVRTGECIPYANIILVSGVTF"
     gene            complement(4376..>5247)
                     /locus_tag="PLB25_13400"
     CDS             complement(4376..>5247)
                     /locus_tag="PLB25_13400"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HOE42611.1"
                     /translation="PSEGETVQGESISYVPGGKGANQAVSCARQGARVSMLGRVGPDH
                     HGETLCEALKQDGIEVNGVLVDPDESTGVAMVMVDEGGRNRIVVIAGANGQLTLDESQ
                     LVQALTDAQFLIMQFEVPMAQVLQAAKVARRLGCKLVLNPSPAKLLPEELWPLVDVMI
                     VNETEARFLTGLSPDTDPQVAAQVGRKLRAKGVAQVVVTLGALGAVATDDAGSTYHPA
                     PHIQVVDTTAAGDTFLGVVTVGLASQQSLTASVDLGIRASTLCVQTMGAQPSIPTLAQ
                     TLQNLSVPAWISL"
BASE COUNT         1120 a         1499 c         1481 g         1147 t
ORIGIN      
        1 gtttgcccag cacgtgctgg gtgtagaact tgaagccata caggtcgagc ttgacggcgc
       61 tccaggagta cgttccgatc aggctgctga agtagtcggt gagctgcgcg ggtgacaagt
      121 tgtcgattgc gtggtcgaag tagtcaccca tgcgccggat ggcgcggctg taggcatcga
      181 tggtcttggg ttgcagacct ttgagtttga ggtgtttgag gtgggtttgg tagcgctgat
      241 caaattcaac cttggtgacg gggttcatgc tgcggtgtcc ttgcgatttg ttgcagaatc
      301 acgccatcga aattgacggg gtgaaggtgc agaataaatc gtctcagcca gcgcaattgc
      361 ggggtggttt actccgcgta gcggcttcgt ccaactattc gtttcaggcg cgacggccct
      421 gacgggccgc ggcctgaact caatcgttag gcgtcatcag aacctcagca accgcatggg
      481 cacttctctc ttcaagtacg tcggcccgtc caatgttgct cgcgtcttcg cgagcaaagg
      541 gttcctcaca ctaaagtgcg gtacgccagc cgagttcaac gatccatacg agttgttctt
      601 aacgatgaac tttcgagagc ggcccgagtt gatcgccttc tacacagatg tggttggtca
      661 actgccacaa ctaccgacga cttgcttttc cagatcgccg tcggtcgctc caatgtgggc
      721 gcactatgca cgcaacctcg aggggttcgt actggaactg gacgagtcca aggtctcagc
      781 ggcttgccca gaaagtgaat tcggggatgt tgactatcgg gataaaccga gctcagccgt
      841 cgccgagaac ctctaccgcg cctacgagat cggaaagttc cgatacatgc actttcttca
      901 gcgctcggtc ttcagcgccg cttactacac aaagcaacga tgctgggcct acgagaaaga
      961 gcgaagaatg attctcgaca agaccgaggt acaggtccgc aacggactga ctttggttga
     1021 ccttccgatc agcacgatgc agtcgctcat cagtggtccc agggcaagcg atgagactgt
     1081 gtcggcatta agggcaatcg ccaaggatgc tggctgccgc ttttggcaga cgaggatcgg
     1141 aagatctaca ggagtcccgt actttgttga cgatgccggc aaacccgctg tcttcaaagg
     1201 cgccgaaatc gcaccggcta aaacatactg cgctagttgc actgaaccca cctccaggcg
     1261 ccaagccaag ctatgttcgt ggtgccgaat agacgactcc catcgcgaga acgcagcgca
     1321 gcgcaactct tatcgcattc ttcaccacta cggccaactc gattcgtatc tgaaatcagt
     1381 caatgagatt gctcgtcgtg cccgtcgaaa gaatgacgcc taacccctcg gtcgaggcga
     1441 ggcccaacgg caaggcagcc cggccgcttc caggttgtgc gtatcatcct tccagcggcc
     1501 gggctgcctc gccgtcggtc ccacctcacc tcgaacgtta aacctcatgg ttaccaagtc
     1561 ggttgctcgc aggcaccact acgttccaca ggcgtatctg gctgcattta cctcaactgg
     1621 agagaaagac ggtcagtttt atgtgctgga cgtggaaacc gggcgtgcct tccgaacctc
     1681 ccctctcaac gttggggcgc agagagattt caatcgtgtg gaagtagacg gtcacccgcc
     1741 cgacgcaatc gaaagcgcac tcgcgccttt tgaaggcagc gctgtggccg caattcgcag
     1801 agtaatcgac agcgaggcat tcccaaacga tagcgactgg aaagcgcatt cactcatggg
     1861 tgccactttg cttttgacca agttgacttt gagcttgagg gtggtctcca gatacagcgt
     1921 gatggaatac atcacccgct gtgcggcacg ctcgcttttg acaagagtca ccatgtcgtc
     1981 cccttgcagg gcgcgccagg cctcaaggcc tgtcccttcg ccagtcacaa acagtccgca
     2041 gggaatgttt gtgtacgggc tcagcataac gtgcaaaacg gtgtccccgc cgctccaact
     2101 cttggtcgag ctggggcagc aggatgtttg ccagcagtgg cgagagtggc ccaccctgtg
     2161 gtgtgcccac ttcgccgggt tcaatgtgct ctccaaccag caccccggcg cgcaggtagc
     2221 gccaatcaaa cgcagcagtc gcttgtcggc aatggagcga gccggcaact tcagcagcat
     2281 gtcatggttg ctcgtcacgc aaacacagca tcaggttgcc acttcgccgc gcctgttgcc
     2341 tgccggtccg cgctgacttt gagtggcggc tatcaaacgc ggcatctggc cgcaacgagt
     2401 cagaagggtc ggctggttcc agcaagtgcg tatcgaccgg tcaccctgaa cgcagccagc
     2461 gcctgcggac tgtggcgggg gggctaattg ctattgttcg gatagctttt agcgcatact
     2521 gtaattgggc tagaggcact tttgttgact atttgcattc cgaagttggc taccggacga
     2581 agctgccaag tagcggcagc ggtcgaccac gcgtaccttc ttgcccttgt tcgtgaagcc
     2641 caggaaggcg cattcgctca tcggtgcaac tttgcttttg atcaagttga gtttgaggtt
     2701 gggtttgccg tcatgtgcca gcttggccat cggcgctctg ggccttctat gcggtttctg
     2761 tccctcgcct tggacatttg cactcgggct tacttcgcac attccccctc gctgccatgc
     2821 ccttgcagtc ggctagtggt taagattgct ctcaataagt ccaattcggt tctcccacag
     2881 gggactttca ccccataagt tcacgcccat gctgggtgca catacgaatc atcagcgcca
     2941 agaaggcaac acgatatgaa aaaggcatct atgaaaatgg ctgaccaaga cattcccgaa
     3001 ttgaagcgcg atgaactgtg tacgggcgta ccaggcaagt gcctgaaaca ctttctgcaa
     3061 ggtagcaatg tggtggtgtt acagcctgaa atccaaaagg ctttccctac ctctgaagca
     3121 gtcaataagg ctctggcgag catgctggcc tttgctcagg aagcacaggt cggaagacgc
     3181 gcaagcgggt cgcagtttag cttcgacatt ggtccaaatg ccttccggtg attcgacccg
     3241 ggtctggttc cctgaaatgc tcgtcgagct gcaaaagcag tggagcagga cacttagttg
     3301 ggaggactgt ggggtgcttt gtgagcgaat gactgaagcc aggacaaagt tgaagctgga
     3361 gaagggcttg caaggcccca tgatgcactg cccacgctgc aatgcggttc acgaaatgcg
     3421 cctggcaccg atcactatcc gctcgctgtt gttcgccctt cgcaagcaag gtgtcctgag
     3481 cgaccccgaa ttgaatcagt tggatgcggc gtggaaacgc taccgagcga agcatcgcct
     3541 tgatggttgc ggcaaggggc gtgaagtacc gaacatgtca ccagcggcga cttctgcttg
     3601 cgttcaaagc ctctgttgac ctcacttttt gagcggcggc tatcaaacgc ggcgtctggc
     3661 cgcaacgagt cagaaaggtc ggctgtttcc aataaatgcg tatcggccgg tcaccctgaa
     3721 tgcaaccagc acctgcgggc tgcagcggga attgcttgct atacttaagc tagctgccag
     3781 cgcatactac aattgggcta gaggcacttt tgttgatcat ttgcattccg aagttggtag
     3841 ccggacgaag cgccaagtag cgtcaacggt cggccacgtg tggctggtta ccgcagatcc
     3901 aattcaacga tgatcatgtg ccttatgccc catagctttg ggcgcacctt ggggtatggc
     3961 ccagacacca gccatcagcg ctaaaacgtc acgccggata ccaaaatgat gttggcgtat
     4021 gggatgcact caccggttcg caccatggcg cgtgcggcac cacacatctt tttgaactcc
     4081 tcatgtccaa ctgtccgagg tgtcagattc aactggtctg tgtaccacgc tggcggtccg
     4141 ccagcgtcta gggcctcagt ggcaatcact gtgctttgaa cctgcagttc agacataacc
     4201 gccctcagca cgtcagccaa tcgaggtgtg ccagggcaca cggccaggtc gatacgctga
     4261 ggcgcgttgg gcccctgcgg gatcggcaga ccggcatcac caataaccag catgtcgccg
     4321 tgtcccattg tggcgatcag atgcgagagt tctgcattga gtaaaagatt gcgtttcata
     4381 aagaaatcca ggccggaact gacaggtttt gcaaggtttg cgcgagagtg ggtatcgacg
     4441 gctgggcacc catggtctga acgcacagtg tcgatgcccg aattcccaga tcgacactgg
     4501 cggtcaagct ttgctggctg gccaagccca cggtgacgac acccagaaaa gtgtcgcctg
     4561 cagccgtggt gtcaacgact tgtatgtgcg gcgctggatg gtaggtgctg ccagcatcgt
     4621 ctgttgccac cgcccccaga gcaccaagcg tcaccaccac ttgggcaaca cctttggcac
     4681 gcaacttgcg cccaacctgc gctgcaactt gcgggtcggt gtcaggtgac aaaccggtca
     4741 ggaaccgtgc ctcggtttca ttcacgatca tgacatcaac cagcggccac agttcctcag
     4801 gcaatagctt ggcgggcgac gggttcagca ccagcttgca gccaaggcgg cgtgcaactt
     4861 tggctgcctg cagcacctgc gccatcggca cttcaaattg catgatcaga aactgcgcat
     4921 cggtcagcgc ctgcaccagt tgagattcat caagcgtgag ttgtccgttg gctccggcaa
     4981 tcacaacgat acggttgcgg ccgccttcgt ccaccatcac catcgcaacg ccggtcgatt
     5041 catcaggatc aaccagcacg ccattgacct caatcccgtc ttgtttgagc gcctcacaca
     5101 gggtctcgcc atgatggtct gggccaacgc ggccaagcat gctgaccctg gcaccttggc
     5161 gagcacaact gacggcctga ttggcaccct tgccgcctgg cacatagctg atcgattcac
     5221 cttgaacggt ctcgccttca gaaggcg
//
LOCUS       DAOSLL010000313         5243 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14097_length_5243_cov_3.805705, whole genome shotgun sequence.
ACCESSION   DAOSLL010000313 DAOSLL010000000
VERSION     DAOSLL010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5243)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5243)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5243
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14097_length_5243_cov_3.805705"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="PLB25_13405"
     CDS             complement(<1..265)
                     /locus_tag="PLB25_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HOE42612.1"
                     /translation="MKTTKIKTATEDGKTVVPASAGEKKKPAPVAQAAAGTDAAVKAS
                     APAKRGRKPKVGLEGAVVDELDMSDIKEDLVGEPVVEVTETTET"
     gene            246..719
                     /locus_tag="PLB25_13410"
     CDS             246..719
                     /locus_tag="PLB25_13410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42613.1"
                     /translation="MILVVFNAGSFGSAGLPLVLLTTLAGASDVTGFLGLEEGMMKPQ
                     NGENRKINGGPKSGAKQEFKEKNWFCLARRMGEHLVCSPCGELAWCSVRIDWPGPEVL
                     LSRLPECNSRIIPFCLTLIACVGCLTGQSRSWHLADTCRNQAPGSDPGEAQFVPD"
     gene            complement(816..2675)
                     /gene="dnaG"
                     /locus_tag="PLB25_13415"
     CDS             complement(816..2675)
                     /gene="dnaG"
                     /locus_tag="PLB25_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HOE42614.1"
                     /translation="MSIPQSFVQELLARADVVEIVGRYVQLKKGGANFMGLCPFHSEK
                     SPSFSVSPTKQFFHCFGCGKSGDAINFLMEHAGMSFVEAVEDLAQQFGMQVPEDDASP
                     QDRARIAQQRQQRATLAEVLEKACLAYQKQLKASPRAIAYFKERGLSGAVAKQFGLGY
                     APEGWHNLSSVFPTYDDPLLVESGLVILQEQEGAQANRYDRFRDRVMFPIRSIKGECI
                     GFGGRVLGDDKPKYLNSPETPVFSKGRELYGLFEARAALREHGYALVTEGYMDVVALA
                     QLGFPNVVATLGTACTADHVQKLFRFTDAVVFSFDGDSAGRRAARKALDAALPWATDV
                     RSVKFLFLPTEHDPDSFIRAHGKEAFARYVSEAVPLSRFLLDAARDGCDLHSAEGRAH
                     LASNARPLWELLPPGALKQQLLAEIADLVQLSNRELKEVWHPGSSKGREASDSYKKSS
                     FQRNEYEGYSPKRHQIEKTGQTLTNSRLPASRADHAARILLDNMAALETLSAEDHHLL
                     CGLSAPHGALFTWLERQFHEHGPLPWVALREGLQGHESAAYARKLMAGYELGNSGNEN
                     EALSELRHLINRMLIEQLKIDETQAIEAAKADPGALQRYRELQARRLVLEAVA"
     gene            complement(3111..3869)
                     /locus_tag="PLB25_13420"
     CDS             complement(3111..3869)
                     /locus_tag="PLB25_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HOE42615.1"
                     /translation="MDIAGKVYIVTGGASGLGEGTARMLAAKGGTVVIADVQVEKGEA
                     VAKEIGGVFVNCDVSIETDAQATVARAAQLGKLMGLVNCAGIAPAEKTVGKNGPHALA
                     GFSRCIAVNLIGSFNMIRLCADAMGKNQPEGTGERGVLISTASVAAYDGQMGQAAYSA
                     SKGGIVGMTLPIARDLARSGIRNMTIAPGIFGTPMMFAFPKEVQDALAASVPFPPRLG
                     TPQDYAKLVVHIIENDMLNGEVIRLDGAIRLAPR"
     gene            4004..4080
                     /locus_tag="PLB25_13425"
     tRNA            4004..4080
                     /locus_tag="PLB25_13425"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4038..4040,aa:Val,seq:gac)
     gene            complement(4191..4724)
                     /locus_tag="PLB25_13430"
                     /pseudo
     CDS             complement(4191..4724)
                     /locus_tag="PLB25_13430"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(4790..4996)
                     /locus_tag="PLB25_13435"
     CDS             complement(4790..4996)
                     /locus_tag="PLB25_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42616.1"
                     /translation="MSLLTTRVTHTPSDFHRYMDQLDELELPYSFVLTSDSEANTMIV
                     ELWLDGSDHNGTDTRATARASPPS"
BASE COUNT         1066 a         1497 c         1508 g         1172 t
ORIGIN      
        1 cggtttcagt cgtctcggtg acctcgacca ccggttcacc gaccaaatct tctttaatgt
       61 cggacatgtc gagttcatcg accactgcgc cttccaggcc aactttgggc ttgcgaccgc
      121 gcttggcagg ggcgctggct ttgactgccg cgtcggtgcc agcggctgcc tgggcgacag
      181 gggcgggctt tttcttttcg ccagcagagg ctggaacaac agttttaccg tcttcggttg
      241 cagtcttgat tttggttgtc ttcaatgcag gttctttcgg ttcggcaggt ttgcccttgg
      301 ttttattgac aacactggcg ggtgcctcag atgtgacagg ttttttaggc ttggaagaag
      361 gcatgatgaa acctcaaaac ggcgaaaaca gaaagatcaa tggcggtccg aagtccggcg
      421 cgaagcagga gttcaaagaa aaaaactggt tttgtctggc aagacggatg ggcgaacatt
      481 tggtatgcag tccttgcggt gaattggcct ggtgcagtgt gcgcatcgat tggccgggtc
      541 cggaggtgtt gctgtcgcgc ttgcctgagt gcaatagtcg gattatacct ttttgcctaa
      601 ccctgatcgc ttgtgtgggc tgtctgaccg ggcaatcgcg gtcgtggcat ttggcagata
      661 cctgtcggaa ccaggcacct ggcagcgacc ccggcgaggc acagtttgtg ccggattgac
      721 cgactcatag ccaaactgcg ggtcacttag ccagtgcaaa atgggctgcg gccggcgcac
      781 cagccgatgc gcgacaaggc cgaccggctc aggattcaag cgacagcttc aagcaccaga
      841 cggcgtgcct gcagttcgcg gtaacgctgc agcgccccgg gatcggcctt ggcggcttca
      901 atcgcctggg tttcgtcgat cttgagttgc tcgatcagca tgcggttgat caaatgacgc
      961 agttcactca gggcttcgtt ctcattgcct gagttgccca gttcgtagcc ggccatcagt
     1021 ttgcgcgcat aagcggcgct ttcgtgcccc tgcaaacctt cgcgcaaggc cacccagggc
     1081 aatggaccgt gctcatggaa ctggcgttcc agccaggtaa acaaggcacc atgcggtgcg
     1141 ctcagaccgc acagcaggtg atggtcttca gcggaaaggg tctccaatgc ggccatgttg
     1201 tcaagcagga tgcgtgctgc atggtccgcc cgactggccg gcaagcggct attggtcaat
     1261 gtctggccgg tcttttcaat ttgatgtctt ttagggctgt agccctcgta ttcattgcgc
     1321 tggaagctac tttttttata gctatcagag gcctcacggc ctttgctgct gcccggatgc
     1381 cacacctctt tgagctcccg gttggagagt tgcaccaggt cggcaatttc ggccaacagt
     1441 tgttgtttca aagcaccagg cggcagcagt tcccacagcg gcctggcgtt gctggccaag
     1501 tgcgcccggc cctcagcgct atgcagatca cagccatcgc gggcggcatc cagcaaaaac
     1561 cggctcagcg gcaccgcttc actgacatag cgggcaaagg cctccttgcc gtgcgcacga
     1621 ataaagctgt cgggatcgtg ttctgtcggc aaaaacaaaa acttgacgct gcgcacatcg
     1681 gttgcccagg gcaaggctgc atccagtgcc ttgcgggcgg cgcgtctgcc agcgctgtcg
     1741 ccatcaaagc tgaacaccac ggcatcggtg aaccgaaaca atttttgtac atgatctgcc
     1801 gtgcaggcgg tgccaagggt ggccaccaca ttggggaaac ccaactgggc caatgccacc
     1861 acatccatgt aaccttcggt aaccagcgcg tagccgtgct cacgcagcgc ggctctggct
     1921 tcaaacaagc cgtacagttc gcggcccttg ctaaacaccg gtgtttctgg cgaattgagg
     1981 tatttgggct tgtcgtcgcc cagcacacgt cctccaaagc cgatgcattc gcccttgatg
     2041 ctgcgaatgg ggaacatgac acggtcgcga aagcggtcgt agcgattggc ctgggcacct
     2101 tcttgctctt gcaggatcac caggccgctt tccaccagca gagggtcgtc atatgtcgga
     2161 aaaacgctgg acaggttgtg ccagccttca ggcgcataac ccaacccaaa ttgcttggcc
     2221 acagcacccg acaagccccg ctccttgaaa taggcgatgg cgcgcggcga tgccttgagc
     2281 tgcttttgat aggcaaggca ggctttttcc agcacctcgg ccagggtcgc ccgctgttgc
     2341 cgctgctgcg cgatgcgggc ccgatcctgc gggctggcat cgtcttcggg cacctgcatg
     2401 ccaaactgct gggccaggtc ttcgaccgcc tcgacaaagc tcatgccggc gtgttccatc
     2461 aaaaaattga tggcatcgcc actcttgcca cagccaaaac agtgaaagaa ctgcttggtc
     2521 gggctgaccg aaaaagacgg cgacttttca ctgtgaaacg ggcacaggcc catgaaattg
     2581 gccccgcctt ttttaagctg cacatagcgg ccgacaattt cgaccacatc tgcacgcgca
     2641 agcagttcct gaacaaaaga ctgggggatg gacatggcgc gattgtagga acgggcagcg
     2701 tgtactgccg atcagcctgg ccttgcgaac tggtcggatg cgaaacagtg cgccccgcgc
     2761 gctggtctgg cttgcagttc gggattttgg ggtaaagcga gaataacttg gaccaaggag
     2821 gtcgtgatgg caagtgccag gctaggagcc tgtcggactt atggaacatc ggctgcgaat
     2881 cgaccgcagc ggtccagttt gcaccggctt tttgacccat agcggggcca tgggcctgca
     2941 aatccgacgc aaactggcct cgctggagcc gattcccgct ttcgacgcca taagtccgac
     3001 aggctcatag gcgcaaaccg cgcggtccaa ctggctacac aagcgatttg caacaacgca
     3061 ttgcgcttgt cagtgcggtc gaactgttca agcaatatct gcttgagacc ttagcgcggg
     3121 gccagacgaa tggcaccatc tagccggatc acttcgccgt tgagcatgtc gttttcaata
     3181 atatgcacca ccagtttggc gtagtcctgc ggcgtgccca gtcgcggcgg gaagggaacg
     3241 ctagcggcca atgcgtcctg tacttctttg ggaaaggcga acatcatcgg tgtgccaaaa
     3301 atgcccggcg caatggtcat gttgcgaatg ccgctgcgcg ccaggtcgcg ggcaatcggc
     3361 agggtcatgc caacaatgcc gcccttggaa gcactgtaag ccgcttggcc catctggcca
     3421 tcgtaggcag ccactgaagc ggtggaaatc agcacaccac gctcaccggt gccttcaggc
     3481 tgatttttgc ccatggcatc ggcacacaag cgaatcatgt tgaagctgcc aatcaggttg
     3541 acggcaatgc atctgctgaa tccggccagt gcgtgcgggc cgttcttgcc aacggtcttt
     3601 tcggcagggg caatgccggc gcagttaacc aggcccatca acttgccaag ctgtgcagct
     3661 ctggcgaccg tcgcttgcgc gtcggtttcg atgctgacat cgcaattgac aaacacaccg
     3721 ccaatttctt tggcaactgc ctcccctttt tcgacttgca cgtcggcgat caccacggtg
     3781 ccacctttgg cagcgagcat gcgcgccgtt ccttcaccca aacccgaagc accgccggtg
     3841 acgatataga ctttgcctgc aatatccatg gaaaactcct caaaacaaga ttgacgttga
     3901 cgtaaacgtc aattatgacc ggtctgctga aagctaaatt tcttctttgc cgtgtcggac
     3961 aagctccgac ttgcggctat actttgccgt tcgcaacagc gacaggcgcg tagctcagct
     4021 ggttagagca ccaccttgac atggtggggg tcgttggttc gagtccaatc gcgcctacca
     4081 attacaagcc ccgtttcccc taaggagacg gggcttttcg gtttctattt cttcatcgta
     4141 gttctccttt agaagtgcgt tagaacaggg gggaatgtca cggttttacg tcacggtctc
     4201 gctgtgttgt cagcgtagct cgccaggaat ccaggcgagt ggtgggcgta gcgcatcacc
     4261 atcctcagat cagcccagcc gcccagcttt tgaaggacct ccaggggtgt gccattttga
     4321 acgtgccagg tggcccaagt gtggcgaaga ccgtgccagg tgaatcccac atagccacca
     4381 gctggcgtct gagagccgac ctttgctcat atgcacgccg ccatgaacgc agttttgatc
     4441 tctttgaccg gatgcccctt gaacgtaaag cagaactcag ggtgctgacc ctggacgctc
     4501 ttgagtgcat tgattgctcc tgttgaaagt ggtatgccaa tagccttctc gcctttcatg
     4561 tcagggccct cggcccaggc gagtttgcgg ttgaggtcaa cgcgtgacca ctgcatcccc
     4621 aacacgttgg cctggcgaag tccggtttcg acggcaaatg tggccatggc caacatgtgg
     4681 ggcggcagct ctgcgcgaag tgcatcccac tgttcgtggg tcagtcattc gcggatgttt
     4741 gaagtctttg ccttgcgtga caccagcttg atgttcacct cggcaagcgt caggatggcg
     4801 gcgacgcgcg tgcggtagcg cgtgtatctg tgccgttgtg gtcggagcca tccaaccaaa
     4861 gttccacgat catggtgttc gcctctgagt cagaggtgag cacgaaagag taaggcagct
     4921 ctagctcgtc gagttggtcc atgtatcggt gaaagtcact cggcgtgtgg gtgactctgg
     4981 tagtgagtaa actcatttct cgatccctcg accctgatct ccttcgaccc tgatctcctt
     5041 cgatcctcag catcatgcgt ccttcgagct ggccaatggt gagtgactcg ccgctgatga
     5101 atctgcttat gtccagctct gtatacgcaa gcacaagatc gcccgcacgc aactcaatac
     5161 ggattgaatc tgccagcctg cccacctttg tgaatggctg tcgctatagg cggaaatatg
     5221 gcggcaaagg gatcagacag gaa
//
LOCUS       DAOSLL010000314         5230 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14136_length_5230_cov_3.904348, whole genome shotgun sequence.
ACCESSION   DAOSLL010000314 DAOSLL010000000
VERSION     DAOSLL010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14136_length_5230_cov_3.904348"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLB25_13440"
     CDS             <1..236
                     /locus_tag="PLB25_13440"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOE42617.1"
                     /translation="QDIGMPTEMALHLRATLLHNAVNNIVALGPKGALTGPAARGDLA
                     LLAVQGKAVAQWNADAGQAYHALSLLAGDLARA"
     gene            974..2461
                     /gene="phaZ"
                     /locus_tag="PLB25_13445"
     CDS             974..2461
                     /gene="phaZ"
                     /locus_tag="PLB25_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011545868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HOE42618.1"
                     /translation="MLYKLYETQRSLMEPFTDLAQSAAKMYANPLSLMGKHPFSQRIS
                     AAYDLVHRLGKDYEKPEFGLTTVDVDGVDVAVHERVEVKKPFCELRRFKRFTDDADTL
                     IKLKDQPAVLIIAPLSGHFATLLRDTVRTMLKDHKVYITDWTNARLVPMSEGEFHLDD
                     YINYVQEFIRHLQKIYGNCHTMSVCQPTVPVLAAVSLMASRGELTPLSMVMMGGPIDA
                     RRSPTAANNLAMNKDIKWFENNVIYRVPTRYAGAGRRVYPGFLQHSGFVAMNPSNHVR
                     SHYDYFQDLIKGDRSSAETHRMFYDEYNAVLDMDANYYLQTIRTVFQDFSLINGTWDV
                     RGVDGKIERVHPEDIKTTALLSVEGQLDDISGSGQTEAVHDICTGVPKAMQEHLEVEG
                     AGHYGIFSGRRWREVVYPIVKAFMLQYQGPVIPVANPLAAVDVTPKPAKRAPKARAAA
                     RAKTPDLASAEITASETDAPPSQVTEEVAATPAKAPRRKAALRQK"
     gene            2458..3138
                     /gene="rsxB"
                     /locus_tag="PLB25_13450"
     CDS             2458..3138
                     /gene="rsxB"
                     /locus_tag="PLB25_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HOE42619.1"
                     /translation="MTVAAASILRMLPQTQCTRCGYPDCAAYAIAVADGVADINQCPP
                     GGTEGVDRLARLTGRPGKPLNPDNGQQGPRKVVHIDEAWCIGCTLCIKACPVDAIVGA
                     NKLMHTVIEAECTGCELCLPACPVDCILVENATGTATGWAAWSQVAADRARDRYAFHC
                     FKRNREETENDERLESKAIMKLADLEHHSQHKDPAVLDKKRIVIAAALARARAKKEGV
                     TPPVPQRP"
     gene            complement(3110..3592)
                     /locus_tag="PLB25_13455"
     CDS             complement(3110..3592)
                     /locus_tag="PLB25_13455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOE42620.1"
                     /translation="MSSTPRPFKTLGIQQIAIGATSKVRLQKLWVDMLGLELTGSFRS
                     ERENVDEDICAMGVGPFKVEVDLMQPVDIDKKPAVHVTPLNHVGIWIDDLPKAVEWLT
                     AQGVRFAPGGIRKGAAGFDICFLHPKGNEEFPICGEGVLIEMVQAPPEVIKAFAALAA
                     "
     gene            complement(3688..>5230)
                     /locus_tag="PLB25_13460"
     CDS             complement(3688..>5230)
                     /locus_tag="PLB25_13460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl/propionyl-CoA carboxylase subunit alpha"
                     /protein_id="HOE42621.1"
                     /translation="VAFNDKEALEGFTSCRNEARNSFGDDRVFIEKFVEEPRHIEIQL
                     IGDSHGNVLYLNERECSIQRRHQKVIEEAPSPFISDATRKAMGEQAVALAKAVKYESA
                     GTVEFVVGKNQDFYFLEMNTRLQVEHPVTECITGLDLVELMIRVAAGEKLPLTQADVK
                     RDGWAMECRINAEDPFRNFLPSTGRLVRFEPPKETMFASDVSQWQGVRVDTGVQDGGE
                     IPMYYDSMIAKLIVHGKDRLEAISKMREALNGFVIRGVSSNIPFQAALLAHPKFVAGD
                     FNTGFIAENYGKGFRAEDVPHTDIEFLVALAAFVNRRYLHRATSITGQLEGHELRVGS
                     EFVVVTMGHEGDGVSYPVHIAEFNDKARIGEITVGGKCYAIHSESKFGWVRIEGTCNG
                     AHFTAQVERGTAKKPLAIRVIHNGTQLDVMVMLKRAADMLALMPHKPPPDMSRYVLSP
                     MPGLLVEVSVQPGQKVVAGERVAVIEAMKMENVLFAAADGVVGKLLAVKGESLAVDQP
                     IVEFA"
BASE COUNT         1178 a         1589 c         1400 g         1063 t
ORIGIN      
        1 ggcaagacat cggcatgccc accgagatgg ccctgcacct gcgcgctacc ctgctgcaca
       61 acgcggtcaa caatatcgtc gcgctcggcc ccaagggcgc attgaccggc ccggccgcgc
      121 gtggcgacct ggcgctgctg gccgtgcagg gcaaggcggt ggcgcagtgg aatgccgatg
      181 ccggtcaggc ttaccatgcg cttagcctgt tggctggcga tctggccagg gcatgacaaa
      241 agttgcgcag ggtaaatttg ttttcatcgc atctccgtcg ctcgcgaaaa gggcgggaat
      301 ggcggcaaat cggccacata aggaaagaac acatttgcac agcaaccctg cctgccgggc
      361 attgacagcg tcccccaacg gttatgtgcg ctgttgtttg acttgcaggg aaaagctgcc
      421 ggaccactag cttgtccgac ttggcgcatc gacagagaga attgaccccg gcaaggccaa
      481 tgtgtgccgg atttgcaggc cgttcgccct gttgcgggtc aaaaacccgg tgcaaaacaa
      541 cccgctgcgg ttggtttgca gccaatgcga cataaggcag gccagttcct ggcacagtga
      601 tcaaacttct gcacggcgcc agcgtgctgc tgcaccgcca ctggccgacg ttgcgaccgc
      661 ccaagggtct gggtggacag gcgccatgcc cgagaggtat ctgtacgcac ctgcgagcag
      721 tttttgccaa agcactggca tccgcttctg ccagcaccgc aaaccgcggt accatgacaa
      781 ccagcgatcc atgcgccctg caaaccagcg ctgaaagaca ccaccgttga aggtgtcacc
      841 agtcgacgat ttgtgccatt gaccgccacc aatcgcataa caaaagccga ctagtttgat
      901 cggatagcta tgcttttcca ttcagtcctg ctatattgca ccgcaacatt gtcaactaga
      961 aagaggttat gctgtgctct ataaattgta cgaaacccaa cgttcgctga tggagccgtt
     1021 caccgatctc gcacagtcgg cggcaaaaat gtacgccaac cccttgtcat tgatgggcaa
     1081 acatccattt tcacagcgca tttcggccgc ctacgatttg gtgcaccgcc tcggtaaaga
     1141 ttacgaaaag cccgagtttg ggctgaccac cgtcgatgtc gacggtgttg atgtcgccgt
     1201 tcacgagcgc gttgaagtta aaaagccatt ttgcgaactg cgtcgcttca aacgttttac
     1261 cgacgatgcc gataccttga tcaagctcaa ggatcaaccg gccgtcctga tcattgcacc
     1321 cctctcgggc cacttcgcga cgctgctgcg cgacactgtc cggaccatgc tgaaagacca
     1381 caaggtctac atcaccgact ggaccaacgc acgcctggtg ccaatgtccg aaggtgagtt
     1441 tcatctggac gactacatca attacgtgca ggaattcatc cgccatttgc agaaaattta
     1501 cggcaactgc cacaccatga gcgtctgcca gccgaccgtg ccagtgctgg cggcagtgtc
     1561 cctgatggcc agccgtggtg aactaacacc cttgagcatg gtgatgatgg gaggcccgat
     1621 cgacgcacga aggtccccca cggcagccaa caacttggcc atgaacaaag acatcaagtg
     1681 gttcgagaac aatgtgatct accgggtgcc gacccgctac gccggtgccg gacggcgtgt
     1741 ctatccaggc tttctgcagc attctggttt tgtcgcgatg aaccccagca accatgtgcg
     1801 cagccattac gactactttc aggacctgat caaaggcgac cgctccagcg ccgagacgca
     1861 tcgcatgttt tatgacgagt acaacgcggt acttgacatg gatgccaact actatctgca
     1921 aaccattcgc accgtgtttc aggatttcag ccttatcaac ggcacctggg atgtgcgcgg
     1981 tgtcgatggc aagattgagc gcgtgcatcc ggaagacatt aaaaccacgg ccctgctgtc
     2041 ggtagaaggc cagctcgacg atatttcggg ctcgggccaa accgaggcgg ttcatgacat
     2101 ttgcactggt gtccccaaag ccatgcagga gcatctggaa gttgaaggtg ctggccacta
     2161 cggcattttt tccgggcggc gctggcgcga agtggtctat ccgatcgtca aggcattcat
     2221 gctgcaatat caaggtcctg ttatccctgt cgccaaccct ttagcagcag tcgatgtcac
     2281 acccaagcca gccaagcgag ccccgaaagc cagagcagcc gcaagagcca aaacgccaga
     2341 ccttgcgtca gcagaaatca cagcgtctga aaccgatgcc ccgcccagcc aagtcaccga
     2401 ggaggtggct gcgacaccgg ccaaagcccc tcgccgcaaa gcggcattgc gccagaaatg
     2461 acggtggcgg cggccagcat cctgcggatg ttgccgcaaa cgcagtgcac gcgctgcggc
     2521 taccctgact gcgctgctta cgccatagcc gttgccgatg gagtagctga cattaaccag
     2581 tgcccgccag gtggcactga aggagtggac cgactggctc gcctgaccgg tcgtcccggc
     2641 aaacccctta atcccgacaa cggccagcaa ggcccgcgca aggtcgtcca tatcgatgag
     2701 gcatggtgta ttggctgcac gctgtgcatc aaagcctgcc cggttgacgc gattgtgggt
     2761 gccaacaagc tgatgcacac ggtgatcgaa gccgaatgca ctggctgtga gctgtgcttg
     2821 ccagcgtgcc cggtagactg cattctggtt gaaaacgcaa cgggcaccgc taccggctgg
     2881 gccgcctggt cacaagtggc ggctgaccgg gctcgtgatc gctacgcttt ccactgcttt
     2941 aagcgcaacc gggaagagac cgagaacgat gaaaggcttg aaagcaaagc catcatgaag
     3001 ttggccgatc tggaacacca ttcgcagcac aaggaccccg cagttctgga caagaagcgc
     3061 atcgtgattg ctgccgcttt ggcccgcgcg cgggccaaaa aggagggtgt tacgccgcca
     3121 gtgccgcaaa ggccttgatg acttcaggcg gtgcctgcac catttcgatc agcacacctt
     3181 caccacaaat cggaaactct tcattgccct tggggtgcaa gaagcagatg tcaaagccgg
     3241 ccgcaccctt gcgaatgccg cccggtgcaa aacgcacacc ctgcgccgtc agccactcca
     3301 ccgcttttgg cagatcatcg atccagatac cgacgtggtt aagcggtgtc acgtgtaccg
     3361 caggtttctt gtcgatatca actggctgca tcagatcgac ctcgaccttg aacgggccga
     3421 cacccatggc acaaatgtcc tcatccacgt tttcccgctc gctcctgaag ctgccggtca
     3481 gctccagccc cagcatatcg acccacagct tttgcaacct gactttgctg gtcgcaccaa
     3541 tggcgatttg ctggatgccc agcgttttga aaggacgtgg tgttgaactc attgtgacct
     3601 ctttggtttt tggttacttg aaaataaata gcatgctacg cagatcacca ttgggccaga
     3661 ctcaatgggt ctggcccaac ggcagactca ggcgaactcg acaatcggct gatcgactgc
     3721 cagactctcg cccttgaccg ccaacagttt accaactacg ccgtcggctg cggcaaacag
     3781 tacattttcc atcttcatgg cttcgatcac ggcaacccgt tcgccagcta ccaccttctg
     3841 acccggttgc acagacacct ccaccagcaa accaggcatc ggcgacagta cataacggct
     3901 catgtctggc ggcggcttgt gcggcatcag ggcaagcatg tcggcagcac gtttcagcat
     3961 caccatcaca tcaagctggg taccgttgtg aatcacacga atggccagtg gttttttggc
     4021 tgtgccacgc tcgacttggg cggtgaaatg ggcgccgttg caagtacctt caatgcgcac
     4081 ccagccaaac ttcgattcac tgtgaatggc ataacactta ccgcccaccg taatttcgcc
     4141 gatgcgcgct ttgtcattga actcggcaat atgcaccgga taagacacgc cgtcgccctc
     4201 atgccccatg gtgaccacca caaattcaga gccaacgcgc agctcgtgtc cctccaactg
     4261 tccggtgatg gatgtcgcac ggtgcaggta acggcgattg acaaaggccg ccagcgccac
     4321 cagaaactcg atgtcggtgt gcggcacatc ttcggcccgg aaacccttgc cgtagttttc
     4381 ggcgataaag ccggtattga agtcacccgc gacaaacttc gggtgcgcca gcagcgcagc
     4441 ctgaaacggg atgttgctgg agacaccgcg aatcacaaag ccattgaggg cttcacgcat
     4501 tttgctaatg gcttccaggc gatctttgcc gtgcacaatc aacttggcaa tcatcgagtc
     4561 gtaatacatc gggatctcgc caccatcctg cacgccggta tcaacccgca caccctgcca
     4621 ctgcgacaca tcagaggcaa acatggtctc tttcgggggc tcgaagcgca ccagacgtcc
     4681 agtggatggc aaaaagttgc gaaacgggtc ttccgcgttg atacgacatt ccatcgccca
     4741 gccgtcacgc ttgacatcgg cctgggtcaa tggcagcttc tcgccagcgg ctacgcggat
     4801 cattaactcg accagatcaa gcccggtaat gcattcggtc accgggtgtt caacctgcag
     4861 gcgggtgttc atttccagaa agtaaaagtc ctggtttttc cctaccacga actccactgt
     4921 gccagcgctt tcatacttca ctgccttggc caaagccacg gcctgctcac ccatggcttt
     4981 gcgggtggca tcgctgatga acggagaagg tgcctcttca atcactttct ggtgacggcg
     5041 ctgaatcgag cattcacgct cgttaaggta cagcacattg ccatggctgt cgccaatcaa
     5101 ctgaatctcg atgtgacggg gttcctcgac aaacttctcg atgaaaaccc ggtcatcacc
     5161 gaagctgttg cgagcctcgt tgcggcaact ggtaaagccc tccagggcct ccttgtcatt
     5221 gaatgccacg
//
LOCUS       DAOSLL010000315         5218 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14173_length_5218_cov_3.883207, whole genome shotgun sequence.
ACCESSION   DAOSLL010000315 DAOSLL010000000
VERSION     DAOSLL010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14173_length_5218_cov_3.883207"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..82
                     /gene="smpB"
                     /locus_tag="PLB25_13465"
     CDS             <1..82
                     /gene="smpB"
                     /locus_tag="PLB25_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856984.1"
                     /note="binds to ssrA RNA (tmRNA) and is required for its
                     successful binding to ribosomes; also appears to function
                     in the trans-translation step by promoting accommodation
                     of tmRNA into the ribosomal A site; SmpB protects the
                     tmRNA from RNase R degradation in Caulobacter crescentus;
                     both the tmRNA and SmpB are regulated in cell
                     cycle-dependent manner; functions in release of stalled
                     ribosomes from damaged mRNAs and targeting proteins for
                     degradation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HOE42622.1"
                     /translation="EHDKRDTIKDREGKREVERAMKSRQR"
     gene            677..848
                     /locus_tag="PLB25_13470"
                     /pseudo
     CDS             677..848
                     /locus_tag="PLB25_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796434.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1162..2007
                     /locus_tag="PLB25_13475"
     CDS             1162..2007
                     /locus_tag="PLB25_13475"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOE42623.1"
                     /translation="MDCKTINYKNIPVQSEVIAGIIRLDVDSASCFRELENLVGSDQG
                     VTTLLLRVVNSSLYSRGRNITTIPLAISVLGFNVVRSLALLALTRSLFSQTKNLLFRT
                     HVWQHSLLTALASQSICGKLVSSSGRDESFVAGLMHDTGKVLLFNHDQNLCLEAISLS
                     IKTGCSSLEAEQHFFGVTHVQIGREAVTQWRLPERFNDYMAFDLSVPRAQYATDVVLL
                     SLAVANTLIKWRGIGGCLTEDTDVQKSTLMALGLNEPLCDSLLDETFVSDLMKVDTYK
                     LFATI"
     gene            2009..3622
                     /locus_tag="PLB25_13480"
     CDS             2009..3622
                     /locus_tag="PLB25_13480"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOE42624.1"
                     /translation="MPSSTTPDQAETHQFTSLGYGVWKNLQSDLERALQYHNLAKFTA
                     PSRLILRDLVESSVKTMHRHIFRQLFETDFGIDLSGNDDALDALYHTETSEHGAQNID
                     RFCITHDQQISVVFPRQDGVIAWIDVPVSWDRSVCQTEELIEAMSFELQLTTRPGKAS
                     GCRLMLMKRSDRQPVPLTHALLEQQHRSTSLSHIFDSLGYGLIHFSTIGEILAVSPSM
                     LSRLGLDVCDASVEIVSEAIPSNFYNDIVWGLALGEPSGKFENYRIRINLPDGGTTSI
                     LFNVSGFRDEMLVIHTLWQKVSIDEGNSQLSEGSILTEVRIHNITRSYVPQLVQEKAR
                     DAIQLGHTQLTNEERFVAVLFCDIVGFTNYVEISAHHESVIDSLNSILRRVAGSVKNF
                     HGSIDKFMGDCVMALFDDPADAVFAACDMQRHAQDINSLRERAGQSVLQLRIGIHWGE
                     VLIGNVGTVERLDWTAIGDVVNTASRIEKNCQPGAILVHQAVRKAIAPIHHGHLSFEE
                     DFYLKVKGKGENLTVCYVSLISTLPNSAC"
     gene            complement(3652..3867)
                     /locus_tag="PLB25_13485"
     CDS             complement(3652..3867)
                     /locus_tag="PLB25_13485"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HOE42625.1"
                     /translation="MPLDWDSDDHSIAHRFGDQWLAELRSAVLVVPSVVAKLEWNAVV
                     NPLHPAAGQLVVSQSQMVIWDQRLFGQ"
     gene            4336..4509
                     /locus_tag="PLB25_13490"
     CDS             4336..4509
                     /locus_tag="PLB25_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42626.1"
                     /translation="MDAAVADRSSLETGFRYESQGETPSFYWVDQGFGDALAGQLPRE
                     TLKKLADLVYHQL"
     gene            complement(4523..>5218)
                     /locus_tag="PLB25_13495"
     CDS             complement(4523..>5218)
                     /locus_tag="PLB25_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003860 - 3-hydroxyisobutyryl-CoA
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HOE42627.1"
                     /translation="KMAMPETTIGLFPDVAGGYFLSRCPGHTGEWLALTGVTLGAADA
                     IELGLADFCADVAQLQPAWDALACINPADPAQLSHWVTMYSIAGSASNIRARGQIDHY
                     FALESVASVLAALQADPGDWASQTVVQLRQRSPLMLHVAFEQIRRARSLNLADELRME
                     RDMMRHCFFPAHLGRSGSQTDTAEGIRALAIDKDNQPRWNPARIEDVSAQMVLPFFTS
                     PWVSHCHPLRGLS"
BASE COUNT         1241 a         1455 c         1350 g         1172 t
ORIGIN      
        1 cgagcacgac aagcgcgaca ccatcaagga ccgtgaaggc aaacgcgaag tggagcgagc
       61 catgaaatcc cgccaaagat gatttttctc aggtaggtgg agtaaatcca tgaactctgg
      121 tgtttgtccc gttgcagacg ctttactgct ttcaattcaa caggagttca ccatgaaatt
      181 tgtggctcta ctgaaatttg agtgggtaca aacttctcaa ttttcgatat gcagccggat
      241 gtgaatcata aaaatagagc atcatctgaa tgcaaaaagc ttgtctgaca tgaaatggaa
      301 aactcattga ggcacaccca tagcagcatt caaacaaaaa tctcgtgcca gaaacggctg
      361 caatgttaac aatcaaaaaa gtagctagct acgcttactg cataagcgat tgacaccgat
      421 ttggcctaag aaaaacgggc tgcctgcatc gccactgccg ccgacctctc cccctgtttc
      481 ttcgagaagg cttggtccgc gtgcggggct gggatgctga ctactcattg ttttttcagc
      541 agcgccgagc aggccccacc ttgagctaac aggttgagcc gccacccttt tggtcaacat
      601 ctctcccgac ttaacccact cgaaattcaa aagaaccaag tttgtttccc ccgcgctgaa
      661 ccttggcgct gtcacgctgg caatcgttgc tggctgtgcg tccacctccc cggcacctgc
      721 caaggcagcc gacggcctgc tgaccggccc caacggcatg acgctgtaca cctttgacaa
      781 ggatgtggcg ggcagtggca agtccacctg caatggcccc ctgcgctgtc aattggccac
      841 cactggtggc cgcagcagca ggttgggtca gtattcaatc ggcacgaaca gtcttatgcc
      901 ttgttcggtg cgtgtagccc atcaacgctc acgccagggt actccactgg aaaaagtctg
      961 aaattggtca tcctcgataa aacgggtaaa gttcgcgctg gccgatggta ggaggattcg
     1021 cgggaatttg agcagaattc aagcagaaca ttgctgcggc caaatcgagg tttttcatgc
     1081 tgatcgatgg cttgtccaga ctgagatact ggaactcgtg cgattgaaat gcctatccgc
     1141 taaaacacga actgcgaata aatggattgc aaaacaatca actacaagaa cattccggtc
     1201 cagtccgagg ttattgctgg catcattaga cttgatgtcg attcggcaag ttgctttcgt
     1261 gaactagaaa acctcgtcgg ttcggatcag ggtgttacca cactgctgct gcgagtagtt
     1321 aattcgtcgc tgtacagtcg tggtcgcaac atcactacca ttccgctggc gatcagtgtg
     1381 cttggattta acgtcgtgcg ctcactggct cttctggcac ttacccgttc gctgttctcg
     1441 caaaccaaaa atctcctgtt tcgcactcac gtctggcagc attcgctgct taccgccctt
     1501 gccagccaga gcatttgcgg caaactcgtt agttccagcg gtcgcgatga gtcctttgtt
     1561 gctggcctga tgcatgacac aggaaaggtg ctgctcttca atcacgacca gaacctctgc
     1621 cttgaggcga tttccctctc catcaaaaca ggctgctcca gtcttgaggc agaacagcat
     1681 tttttcggtg taacccatgt gcagatcggg cgcgaggcgg taactcagtg gaggctgccg
     1741 gagcgattca atgactacat ggcatttgac ctctctgtgc cacgcgccca atatgcgaca
     1801 gatgtcgtgt tgctcagcct ggcagttgca aacaccttga tcaaatggcg cggtatcgga
     1861 ggctgcctga ccgaggatac cgacgtacaa aagtccacac tgatggcgct tgggctcaat
     1921 gaaccacttt gcgattccct gttagacgag acctttgttt ccgacctgat gaaagtcgac
     1981 acctacaaac tctttgccac gatttaacat gccctcatcc acaactccgg accaggccga
     2041 aacccatcag ttcacctcac ttgggtacgg cgtctggaag aatctccagt ccgatctgga
     2101 acgggcgctt caatatcaca atcttgcaaa atttactgca ccttctagac ttattctccg
     2161 tgatctggtt gaaagctcag tcaaaaccat gcaccgtcat atattccgtc agctttttga
     2221 aaccgacttc ggtattgacc tctccggcaa cgatgacgct cttgacgctc tctaccatac
     2281 cgagacaagt gaacatggtg cacagaatat tgaccgtttt tgcataaccc acgatcagca
     2341 aatttcggta gttttccccc gccaagacgg cgtgatagcg tggatagacg ttcctgtaag
     2401 ctgggacaga tcagtctgtc agactgaaga actcatcgaa gcgatgagtt ttgaactgca
     2461 gttgaccacc cgtccgggga aggcatctgg atgtcggctg atgctgatga aacgcagtga
     2521 ccgccaacct gtgccgttaa cacacgccct acttgaacag caacacagat caaccagcct
     2581 gagccacatc tttgacagtc tcggctacgg cctcatccac ttttctacca ttggcgaaat
     2641 tcttgcggta tctccctcca tgctcagccg gcttgggttg gatgtttgcg acgcctcggt
     2701 cgaaatcgtg tcagaggcga ttccctcgaa tttctacaat gacattgtct ggggccttgc
     2761 cttgggcgaa cccagtggca aatttgaaaa ctaccggata cgcatcaatc tgcctgacgg
     2821 tgggactacc tcgatcctct tcaacgtcag cggtttccgc gatgaaatgc tggtcattca
     2881 cacgctctgg cagaaggttt ccattgatga gggcaatagc cagctttccg aaggctccat
     2941 cctgaccgaa gtcagaatcc acaacattac gcgaagctat gtgccgcaac tggtacagga
     3001 aaaagcacgt gatgccatcc agctaggcca tacccaacta accaatgaag agcgcttcgt
     3061 ggcagtgttg ttctgcgata ttgtcggctt caccaactat gttgaaatca gcgcacacca
     3121 cgaatcagta atcgactcac taaattcgat acttcgccgc gtcgccggat cggtaaagaa
     3181 ctttcatggc tcgatcgaca agttcatggg cgactgtgtc atggctttgt tcgatgaccc
     3241 tgcggatgcc gtatttgccg cctgcgacat gcagcgccac gcccaagaca tcaacagctt
     3301 gcgcgagcga gccgggcagt ccgtcttgca gttgcgcatt ggcatccact ggggcgaggt
     3361 gttgataggc aatgttggaa cggtcgaacg actcgactgg acagccattg gcgatgtggt
     3421 gaataccgcg tcacgcatcg aaaaaaactg ccagccaggt gccatactcg ttcatcaggc
     3481 agtgcgcaaa gcgatcgcac cgatccatca cggacacctc agcttcgagg aagactttta
     3541 ccttaaagta aagggtaaag gcgagaacct gactgtttgc tacgtcagtc tgatctcgac
     3601 attgcccaac tctgcatgct gaaggtaacg gtgtagttca aactgaggga gttattgacc
     3661 aaacagacgt tgatcccaaa tgaccatttg actctgcgac acaacaagct gccctgcagc
     3721 aggatgaagc ggattgacca ccgcattcca ttccagcttg gcaacgacag acggcacgac
     3781 caagaccgca cttctaagct cggctaacca ttgatcgcca aaacgatgtg caatggaatg
     3841 atcgtcgcta tcccagtcaa gcggcagtgc ctgagagtct gctcagcttc agtcagcgca
     3901 gaccattgtg tgagcgctgc gttgataagt tgatgcccca aggctgactg agcacaacat
     3961 ccgcttttgt atgcgctgcg agaagtccag cggcggctgg gccgcagtgc gcgctttcgc
     4021 caacagcggt caagttgatg cccaggtcac gctgaacaag cccaacaaat gggactgcca
     4081 agactatcga tgctctggtg tcacaccaca ggtcaggttg gtcaggtgtg tgacacctga
     4141 tggacttgtt cgggtgatgg caaccaatac gccaatcagc caatttccga cagtcgtttt
     4201 tagtgagctt tatcatgagc gctggcgtat cgaggaggcg ttcaagcgac tcaagcatca
     4261 ggtcaagctc gaaagtgtct ccgtacgaga caatcgatta ccgcgtcacg cttcgactct
     4321 acttatctgg gcgcagtgga tgctgcagtg gcagaccggt caagcctgga gaccggtttc
     4381 cgctacgagt cacagggcga aacccccagc ttttactggg tcgatcaggg ctttggcgac
     4441 gcgctggccg ggcaactgcc gcgcgagacg ctgaagaaac tggccgatct ggtctatcac
     4501 cagctctgaa gtgcgggtgc catcagctca agccccgcag cgggtggcag tggctgaccc
     4561 aggggctggt aaaaaaaggc agcaccatct gggcgctgac atcttcaatg cgcgccgggt
     4621 tccagcgcgg ctggttgtcc ttgtcaatgg ccagcgcgcg gatgccttcg gcggtatcgg
     4681 tctggctgcc actgcgcccg agatgcgccg gaaaaaaaca gtgccgcatc atgtcgcgct
     4741 ccatgcgcag ttcatcggcc aggttgaggc tgcgggcgcg gcgaatctgc tcgaacgcca
     4801 catgcagcat cagcggcgag cgctggcgca actggacaac ggtctggctg gcccagtcgc
     4861 ctggatcggc ttgcaaggcg gctagcacgc tggccacact ttccaacgca aaatagtggt
     4921 caatttggcc tctagccctt atgttgcttg cgctaccagc tattgaatac atagtaaccc
     4981 aatgtgacaa ctgagccggg tctgccgggt tgatgcaagc cagcgcgtcc caggctggct
     5041 gcaactgcgc cacatcggca caaaaatcag ccaaacccag ttcgatggca tcggcagcgc
     5101 ccagggtgac cccggtcagc gccagccatt cgccagtgtg cccagggcag cggctcagaa
     5161 aatagccgcc agcaacatcc ggaaacaggc caatggtggt ttccggcatg gccatttt
//
LOCUS       DAOSLL010000316         5176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14329_length_5176_cov_4.566296, whole genome shotgun sequence.
ACCESSION   DAOSLL010000316 DAOSLL010000000
VERSION     DAOSLL010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14329_length_5176_cov_4.566296"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLB25_13500"
     CDS             complement(<1..252)
                     /locus_tag="PLB25_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009241623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE42628.1"
                     /translation="MMVKIVDVAKQAGVSTASVSRVMSGLPGISDETRARVNAAVKLL
                     GYRPALAARRLRSGKTDTLGLIVSDIRNPFFTEISRAVED"
     gene            488..>1357
                     /locus_tag="PLB25_13505"
     CDS             488..>1357
                     /locus_tag="PLB25_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HOE42629.1"
                     /translation="MTTACVQLNAQASNKTEAVRLAGQLLVNAGYIAPAYIDSMLRRE
                     AVANTFLGAGVAIPHGMVEDRHHILHTGVAVVQFRSGVIWKLGDQAQLVVAIAARSDE
                     HIVLLRRLTRLMQRPQSIESLVATDDAQLIVSTLADEPMPVAAPVAEVLEWPVDAQAD
                     WTLDYPNGLHARPATRWVDTAKRFACDIRVYKAHEFADAKALTSLLTLGVTRGDSLRL
                     AARGPDARRAVDALLELVRSLSADEKADAERARRNALAARRSAPDWLPSASPQAVYGI
                     GASPGLAIGTLVRH"
     assembly_gap    1358..1367
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1368..2974
                     /gene="ptsP"
                     /locus_tag="PLB25_13510"
     CDS             <1368..2974
                     /gene="ptsP"
                     /locus_tag="PLB25_13510"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020832734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HOE42630.1"
                     /translation="VSSQFEVSDSPGDVVADGEALEAALLAISQELEILENQTRTRLG
                     APQAAIFAAQRELIADVDLLREALASILRGHGPAWAWQRALQARIEKLQAVADPLLAA
                     RALDLRDVGERVLAHMLGIERQCLVLTKPSILVAEDLTPSDTLQLDMNFVVGLAISSG
                     GPTSHTAILARTLGLPAVVAAGSALLAAPEGQSAVIDGSVGAVYLGVSEADQDTAAAV
                     IARQLAARNALREGRYLPATTTDGHTVEVAANVANAKQASVALEAGAEGIGLMRTEFL
                     FLERNSAPDEEEQYRVYRDMTEVMGGRTLIIRALDIGGDKKVPYLNLPKEENPFLGVR
                     GARLLLIRQDLLYTQLRALYRAGAHGPIRIMFPMVTHTSEIEALRIHCERARAEVNGP
                     QLKIGIMVEVPAVAVMADKFAPLVDFFSVGTNDLTQYTLAIDRQHPELAPQADSLHPA
                     VLDLIARTVAAAKKHGTWVGVCGGLAGDPLGARILTGLGVDELSMSAQDVAAVKAALR
                     GDSFSAMQALAQRALAAGSADEVRAL"
     gene            2971..3951
                     /gene="pfkB"
                     /locus_tag="PLB25_13515"
     CDS             2971..3951
                     /gene="pfkB"
                     /locus_tag="PLB25_13515"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014972255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HOE42631.1"
                     /translation="MNTTLHSSLPQVIAVALNPALDHTIEVEHLRPGEVNRALRMQVD
                     VGGKGINVASCLADFGIPSAVTGQIGRDNAVLFEALFRAKEIDNQFVYLDGLTRINTK
                     LVDRASGDTTDINMPGPALVADDMQALRESLLTRLDALAGPTSWVVLAGSLPPLWPAD
                     TYQSLIEHVHRLGAKVLLDASGAALAAGIAAAPDIIKPNRAELAELIGRQLDDTPAIV
                     AAARELLARPNAPQTVIVSMGGDGALFVNRQQALLAHPLQTPLISTVGAGDAMVAGIV
                     AARLADLALPECARLASAFSAAKLARLGPHLPSPETVRALAQTVQISPLA"
     gene            3994..>5176
                     /locus_tag="PLB25_13520"
     CDS             3994..>5176
                     /locus_tag="PLB25_13520"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HOE42632.1"
                     /translation="MSKIIAVTACPTGIAHTIMAAEALQQAADRTGHSIQVETQGSGG
                     TRNTLTAADIAAADAVIIAADTHVSTERFASKRLLEVGTGPTIRDAEAVIRQALSVAM
                     PAARPGVAPARDPAMPQIVAITSCPTGIAHTFMAAEGLESGAKANQYSIKVETQGSVG
                     AQNTLTAAEIASAEIVLIAADTNVDMSRFAGKRIYETSTKAAIKDGAAVVRTAWEQAK
                     AASPATGEAYVDQVKAAKAQQSASRTGAYKHLMTGVSYMIPFVVAGGLLIALGFALGG
                     IYVYEDSHKGSLAWTLFTIGAKSGFALMVPILAGFIAHSIADRPGLAPGMIGGMIAAS
                     IGAGFIGGIAAGFLAGYTVKFLNDKIQLGRNLDGLKPVLILPLLGTAIVGLLMFYVVG
                     GP"
BASE COUNT          959 a         1644 c         1590 g          973 t
ORIGIN      
        1 gtcttccact gcgcggctga tctcggtgaa gaacgggttg cggatgtcgg aaacgatcag
       61 accaagtgtg tccgtcttgc ccgatcgcag tcggcgtgcc gccagagctg gcctgtaccc
      121 cagcaatttg acggctgcgt tgacgcgtgc ccgcgtctca tcgctgatgc ctggcagccc
      181 cgacatcacc cgcgacaccg aggcggttga taccccggcc tgcttggcca catccacgat
      241 tttgaccatc atgcctcctg aaaatgtaat cgattgcgac aattgtgcac caagtcaagg
      301 gaacatcttc aataccgacc ctttcaaagc ctttttgcgt gaacaaattt ttaaaatcgc
      361 aaaaaatcca ccacaaagcc gtgatttgtc tgataatcaa aaatgtaatc gattacatta
      421 atttgtaatc gattacatca acccttcaag aacgccatca accagcaagg agacacggat
      481 ggaaaccatg acgaccgcct gcgtacaact gaatgcccag gccagcaata aaaccgaagc
      541 agtgcgcctc gccgggcaac tgctggtgaa tgcgggctat attgccccag cctatattga
      601 cagcatgctc aggcgcgaag ctgtcgccaa cacctttctg ggggcaggag tcgcgattcc
      661 gcacggcatg gtggaagacc gtcaccacat cctgcatacc ggcgttgcgg tggtccaatt
      721 tcgcagtggc gtgatctgga aacttggcga tcaggcacag ttggtggtgg cgattgccgc
      781 caggtcagat gaacacatcg tgctgctacg ccgcctgacc cgcctgatgc agcggcccca
      841 gagcatcgag agcctggtgg ccaccgatga cgcgcaactg atcgtcagca ccctcgccga
      901 tgaaccgatg ccggttgccg ccccggtagc cgaggtgctg gaatggccgg tggatgccca
      961 ggctgactgg acgctcgact accccaacgg cctgcatgcc cgcccggcca cccgctgggt
     1021 cgatactgcc aagcgctttg cctgtgacat ccgcgtctat aaagcgcatg aatttgccga
     1081 tgccaaggcg ttgaccagct tgctgacgct gggtgtgacg cgcggcgaca gtctgcgcct
     1141 ggcagcgcgt ggtccggatg cccggcgcgc cgtcgatgcc ctgctggagc tggtgcgctc
     1201 cttgtcagcc gatgaaaagg ccgatgccga acgggcccgc cgtaacgccc tggccgcccg
     1261 ccgcagtgcc cccgactggt tgcccagcgc cagcccgcaa gccgtttacg gcattggcgc
     1321 aagccccggc ctggccatcg gaactttggt gcggcatnnn nnnnnnnatg tcagtagcca
     1381 gttcgaggtg tccgacagcc ccggtgacgt agtggccgat ggcgaagcgc tggaagctgc
     1441 gctgttggcg attagccaag agcttgagat tctggaaaat cagacccgca cccggcttgg
     1501 cgcgccacaa gccgcgatct ttgcggccca gcgtgaactg atcgccgatg tcgatctgct
     1561 gcgcgaagct ttggccagca ttttgcgcgg tcatggtccg gcctgggcct ggcagcgcgc
     1621 tttgcaagcc cgcattgaaa agcttcaggc agtggcagac ccgctgctgg cggcccgcgc
     1681 ccttgacttg cgcgatgtcg gcgagcgcgt gctggcgcat atgctgggga tcgagcgcca
     1741 gtgcctggtg ctgaccaaac ccagcattct ggtggcagaa gatctgaccc cgtccgatac
     1801 gctgcagctc gacatgaact ttgtcgtcgg cctggcgatc agcagcggcg ggccaacctc
     1861 gcacaccgcg attctggccc gtacgcttgg cctgccagcg gtggtagccg ctggcagtgc
     1921 gctgctggcc gcccccgagg gtcagagcgc ggtgatcgac ggcagcgtcg gggccgttta
     1981 tctgggggtg tccgaggccg atcaggacac agcagcggcc gtcatcgccc gccaactggc
     2041 agcccgcaac gccttgcgcg aaggtcgcta tctgccggcc accaccaccg atggccacac
     2101 ggttgaggtc gccgccaatg tggccaatgc caaacaggcc agcgtggccc ttgaagccgg
     2161 ggccgaaggc attggcctga tgcgcaccga gtttttgttt ctggagcgca acagcgcacc
     2221 cgacgaggaa gagcagtacc gcgtctaccg tgacatgacc gaggtcatgg gcggacgcac
     2281 cctgatcatc cgggcactgg acattggcgg cgacaagaaa gtgccctacc tgaacctgcc
     2341 caaggaggag aacccgtttc tgggcgtgcg cggggccaga ctgctgctga tccggcaaga
     2401 cttgctttac acccagttgc gcgcgcttta ccgcgctggt gcccatggtc cgatccgcat
     2461 catgttcccg atggtcaccc ataccagtga aatcgaggcc ctgcgcatcc attgcgaacg
     2521 cgcccgcgcc gaggtcaacg ggccgcagct caagatcggc atcatggtcg aagtgccagc
     2581 ggtggcggtc atggccgaca aatttgcgcc gctggtcgat tttttctcgg tcggcaccaa
     2641 cgacctgacc cagtacacgt tggcgattga ccgccagcac cccgagctgg caccgcaggc
     2701 tgacagcctg cacccggccg tgctggacct gattgcccgt accgtagctg ctgccaaaaa
     2761 gcacggtacc tgggtcggtg tctgtggtgg cctggccggc gacccgctgg gcgcgcgaat
     2821 tctgaccggg ctgggtgtcg atgaactgtc gatgagcgca caggacgtgg ccgccgtcaa
     2881 ggcagctttg cgcggcgact cattcagtgc catgcaggcg ctggcacaac gcgcactggc
     2941 agcaggcagc gccgatgagg tgcgcgcact atgaatacca cgttgcacag cagcctgccc
     3001 caggtgattg cggtggcgct gaacccggcg ctggatcaca ccatcgaggt cgagcatctt
     3061 cgccctggtg aagtcaaccg cgccttgcgc atgcaggtcg atgtgggtgg caagggcatc
     3121 aacgtcgcct cgtgtctggc ggactttggc atccccagtg ccgtcaccgg ccagatcggg
     3181 cgcgacaacg cggtcctgtt tgaagccctg tttcgagcca aggagattga caaccagttt
     3241 gtttatctgg acggactgac ccgcattaac accaagctgg tggaccgtgc cagtggtgac
     3301 accaccgaca tcaacatgcc cggcccggcg ctggtcgcag acgacatgca agccctgcgt
     3361 gaaagcctgc tgacgcgcct ggatgccctg gccggcccca ccagttgggt ggtgctggca
     3421 ggcagcttgc cgccgctgtg gcccgctgac acctaccaga gcctgattga gcatgttcat
     3481 cgcctgggtg ccaaagtgct gctcgatgcc agcggtgccg ccctggccgc aggtattgct
     3541 gcagcgccag acatcatcaa gccaaaccgg gccgaactcg ccgaactgat cggtcgccag
     3601 ttggacgata cccccgccat cgtcgctgct gcgcgcgaac tgctggcccg cccgaatgcg
     3661 ccgcagacgg tgattgtgtc gatgggtggc gatggcgcgc tgtttgtcaa tcgtcaacag
     3721 gccctgctgg cgcaccccct gcagaccccc ttgatcagca ccgtgggggc tggcgacgcg
     3781 atggtggctg gcatcgtcgc agcgcggctc gccgatttgg cgctgcccga atgtgcccgt
     3841 ctggcgagcg ccttttctgc tgccaagctg gcccggcttg gcccccattt gccttcgcct
     3901 gagacggtgc gggcgctggc acaaaccgtc cagatcagtc ctcttgcctg accccgaagt
     3961 tcgcatttca cccttactca aaggaacatc atcatgtcaa aaataatcgc cgtgaccgct
     4021 tgcccgaccg gcatcgctca caccatcatg gccgccgagg ccctgcaaca agccgctgac
     4081 cgcaccgggc acagcattca agtggaaacc cagggttctg gcggcactcg caacacgctg
     4141 accgccgccg atattgctgc ggctgacgca gtgatcattg ctgccgacac gcatgtcagc
     4201 acggagcgct ttgccagcaa gcgattgctt gaagttggca ctggcccgac catccgcgat
     4261 gccgaagcgg tgattcgtca ggcgctcagc gtggccatgc ccgcagcccg gcccggcgtt
     4321 gcgccagcgc gtgacccggc catgcctcaa attgttgcca tcacgtcctg cccgaccggc
     4381 attgcccaca ccttcatggc ggccgaaggg ctggagagtg gtgccaaggc caaccagtac
     4441 agcatcaagg tcgaaaccca gggctcggtc ggtgcccaga acacgctgac agcggctgaa
     4501 attgccagcg ccgagattgt gctgatcgcc gccgatacca atgttgacat gagccgcttt
     4561 gccggcaagc gcatttacga gaccagcacc aaggctgcca tcaaggacgg tgccgccgta
     4621 gtgcgtaccg cctgggagca ggccaaagcg gcaagtccgg caactggcga ggcgtatgtt
     4681 gaccaggtca aggccgccaa ggcgcagcaa agtgccagcc gtaccggggc ctacaagcat
     4741 ctgatgaccg gtgtctcgta catgattccg tttgtcgtcg ccggtggcct gctgattgcc
     4801 ctgggctttg cgctgggtgg catttatgtc tatgaggatt cgcacaaagg cagcctggcc
     4861 tggacgctgt tcacgattgg tgccaagagc ggctttgcgt tgatggtgcc gattctggcg
     4921 ggctttattg cccactcgat tgccgaccgg cctggtctgg caccgggcat gatcggcggc
     4981 atgatcgccg cgtcgattgg tgctggcttt atcggcggca ttgcggctgg ctttctggcg
     5041 ggctacacgg tcaagtttct caacgacaag atccagctcg gccgcaatct ggacggactc
     5101 aagccggtgc tgattctgcc cctgctgggt actgccattg ttggcctgct gatgttctat
     5161 gtggtcggtg gcccgg
//
LOCUS       DAOSLL010000317         5155 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14419_length_5155_cov_4.544118, whole genome shotgun sequence.
ACCESSION   DAOSLL010000317 DAOSLL010000000
VERSION     DAOSLL010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5155)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14419_length_5155_cov_4.544118"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(217..939)
                     /gene="motB"
                     /locus_tag="PLB25_13525"
                     /pseudo
     CDS             complement(217..939)
                     /gene="motB"
                     /locus_tag="PLB25_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465966.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
     gene            complement(958..1815)
                     /gene="motA"
                     /locus_tag="PLB25_13530"
     CDS             complement(958..1815)
                     /gene="motA"
                     /locus_tag="PLB25_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HOE42633.1"
                     /translation="MFVIIGWLVVLGCVFGVFIIHGGNISVILKALPFEMITIGGATI
                     GAFLANNQMKVVKSTMRGLGACFKGSKYTKARYMELMALLFDILQKARKEGLMAIEND
                     VENPHESALFKKYPTVGSDHHVVEFITDYLRMMVSGNLNAHEIESIMDSEIETHHHEE
                     HAAVAAIQRIAGGLPAFGIVAAVLGVVNTMGSVGQPPAVLGGMIGSALVGTFLGILLA
                     YAFAEPLAGLLEQKNEEAGKELQCIKTVLLASMQGYNPSTAIEFGRKVLYSTERPTFA
                     ELEAHVKKK"
     gene            complement(1981..2517)
                     /gene="flhC"
                     /locus_tag="PLB25_13535"
     CDS             complement(1981..2517)
                     /gene="flhC"
                     /locus_tag="PLB25_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HOE42634.1"
                     /translation="MATKSVLKDSKQVERAVALIQLGARLQVLESETDLSYERLLRLY
                     KEVAGRSPSKGQLPFSTEWFLTWQPNIHASLFQNTYEYLTKVSALEDIDAIMKAYKLY
                     CEQIAACGVEPLLSITRAWRLVKFVDNNMLSMTKCSKCGGHFVSEAYENSRHYECGLC
                     NPPARAGKGASIGGILLH"
     gene            complement(2546..2872)
                     /gene="flhD"
                     /locus_tag="PLB25_13540"
     CDS             complement(2546..2872)
                     /gene="flhD"
                     /locus_tag="PLB25_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HOE42635.1"
                     /translation="MTNEQMLNEIREANMTYLMLAQNLIRQDKAEALFRLGMSEESAD
                     LIGALSPAQIMKIASGNMLLCRFRVDDDMVWNLLTNHSVNKVDNDATTRLHASILMSG
                     RFAETM"
     gene            complement(3080..4621)
                     /locus_tag="PLB25_13545"
     CDS             complement(3080..4621)
                     /locus_tag="PLB25_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HOE42636.1"
                     /translation="MASTINTNISSMTAQRNLSQSQGSLATSMQRLSSGLRINSAKDD
                     AAGLAIADRMTSQIRGSTQAARNANDGISLAQVAEGALGAAGTILQRVRELAVQSANA
                     TNSASDRAALNAEVGQLTSELDRIAKSTEFNGQKLLDGSFGSATFQVGANANQSITAT
                     TANFSTNKYGNNRIGSVVSANAGGAGDLVTGSTANAVASSAGAAAAITSRITGGGTVI
                     VNGASGSATIPVSAGDSAKTVAAAINAQSGTTGVAATARTEIDLGTFAANAGYTLQVT
                     SDNSTAVSIAFTVGSPANTPDSLAAGITAFNDKTSQTGLSAKLNSAGTGITVTNATGN
                     DILIGNISATGSNVTVGTTVAVASNGAPAAAANGAYVTGALTLDSDKTFGVVATGAQA
                     AAATGYFNAAAAGSQLQAASTLDVSTVAAATRSLSTVDSALATVNGQRASFGALQSRF
                     ETTISNLQTTAENLSASRSRIQDADFALETANLSRTQILQQAGTAMVAQANQLPQGVL
                     ALLRQ"
BASE COUNT         1043 a         1402 c         1505 g         1205 t
ORIGIN      
        1 tgccggacgc tgaccttggg cttctggtcg gtgccgatct cgttgaggac gatgtcgacg
       61 ttgccgtcgc cgggcgtgcc gtcggggcgg aagaagaaac gcaggccctg ctggaattcg
      121 ccggatcccg cgtcgtagtc gtacatggcc gggtcgaaca cgtagccttt cgggagactg
      181 tattcagggc cgaccatcca gccgtactcg tggttgggca cgaagctccc ggcgggtggc
      241 gttggcgcgg tctgctgaaa gctcccagtt gctgtaaccc ctggccgcat tgccgtaggc
      301 tgcgcggtcg gtgtgcccgt ccagactgat cttgttctct acatccgtca gcgcaatgcc
      361 gatttcgcgc agaatttccc gcatataggg tttgactacc gcgctgccac tgtcgaacat
      421 gggcctgcgc tggtcgtcca cgatctggat ctgcaagccg tccggggtaa tttccaggcg
      481 gatctgggag ttgaattccg agagcttggg gttgttggaa atgacacctg cgatttttgc
      541 gctcaaggcg gccagccgct tggcatcgcg catggcggtt tccaccttgt tcttgtcgcc
      601 cgacattttc ttggcggact tgtcggcacc atcaccgcgc ttggactgtc cggcggactg
      661 ggtgaggtcg ttgccaccac cgttgatgac gctgttgctg gcgccggccc ccgacccacc
      721 ctgtaatgcc accttgagcg gggactggaa gtagtcggaa atgccttttt tgtcgccttc
      781 ggtcgtgctg cccagcagcc acatcagcag aaagaaggcc atcatggccg tcacgaagtc
      841 ggcatacgca atcttccagg cgccgccatg cgctgcgtgg ccacccttct tgacccgctt
      901 gatgatgatc ggctgaagtt tcttggcgtc tcctgccatg tctagcgtct catggcctta
      961 ttttttcttg acgtgggctt ccagctccgc gaacgtcggg cgctcagtgg aatacagaac
     1021 cttgcgacca aattcgatgg cggtggaagg gttgtagccc tgcatgctgg ccagcaaaac
     1081 ggtcttgatg cactgcagct ccttgccggc ttcctcgttc ttttgctcca gcaggccggc
     1141 cagcggctcg gcgaaagcgt aggccagcaa gataccgagg aacgttccca ccagcgcgga
     1201 gccgatcatg ccgcccagca ccgccggggg ctggccgacc gagcccatgg tgttcaccac
     1261 gcccagcacc gcagccacaa taccgaaagc cggcaatcca ccggcgatcc gctggatggc
     1321 ggccaccgcg gcatgttcct catggtggtg cgtttctatt tcgctgtcca tgatcgattc
     1381 gatctcatgg gcgttgaggt tgcccgagac catcatgcgc aggtaatcgg taatgaattc
     1441 gaccacatgg tggtccgagc ctaccgtggg gtatttcttg aacagtgccg attcgtgcgg
     1501 attctcgaca tcgttttcga tggccatcag gccttccttg cgcgctttct gcaggatgtc
     1561 gaacagcagc gccatcaact ccatatagcg cgccttggtg tatttgctgc ctttgaagca
     1621 ggcacccagt ccgcgcatgg tggacttgac caccttcatc tggttattgg ccagaaacgc
     1681 gccaatcgtt gcaccgccaa tggtgatcat ctcgaagggt aatgccttca agatgacgga
     1741 gatgttgccg ccgtggatga tgaacacgcc gaacacgcag cccagaacga ctagccagcc
     1801 aatgattacg aacataaatg cattgtgaca gggaagccag gggctccaag ggttgaagag
     1861 gcaggccttt gggggcctgt ctctatcata tcgtcaggca gctgggcgac ttgaattttg
     1921 taagaaatgc gggtctagcc cccgtcaata ttgcgcaggt agctatcaaa aaaatagcaa
     1981 tcagtgcaac aggatgccgc cgatggacgc acccttgccg gcgcgggcgg gcgggttgca
     2041 caggccgcac tcgtagtgcc gtgagttttc gtaggcttcg ctgacaaagt ggccaccgca
     2101 cttggagcat ttggtcatgg acagcatgtt gttgtcgaca aacttcacca gacgccaggc
     2161 acgggtgatg gacagcagcg gttccacgcc gcaggcagca atctgctcac agtagagctt
     2221 gtaggccttc atgatggcgt caatgtcttc cagcgcgctc accttggtca ggtattcgta
     2281 ggtgttctgg aacagcgatg catggatatt gggctgccat gtcagaaacc actcggtgga
     2341 gaagggcagt tgccccttgc tgggtgagcg accggccacc tctttgtaca ggcgcaacaa
     2401 gcgttcgtag gacaagtcgg tttcgctctc cagcacttgc aggcgggcgc ccagctgaat
     2461 cagtgcaacg gctcgttcaa cctgcttcga atctttgagt acgcttttgg tggccatggt
     2521 ggttctctcc ggaaaatggg gggggttaca tcgtctcggc gaaacggccg gacatcaaaa
     2581 tgctggcgtg caagcgtgtg gtggcatcgt tgtctacctt gttcaccgag tggttggtca
     2641 gcaggttcca gaccatgtcg tcatccaccc ggaagcggca cagcagcatg ttgcccgatg
     2701 caattttcat gatctgtgca ggcgagagcg cgccgatgag atcggcagac tcttccgaca
     2761 tgcccaggcg gaacagcgct tcggccttgt cctggcgaat caggttttgc gccagcatca
     2821 ggtaagtcat gttggcttcc cggatctcgt tcagcatttg ctcgttggtc attttcagct
     2881 cctgtttagg gcggcaactc gtttgaatca ccatgggctg aattctggaa tgggcagcgc
     2941 aaaacttgaa tcggaaaacg gctaatgcgc gcgtcggaaa cgcagaaacg gcgtgtaggg
     3001 aaaactccta cacgccgaat atgaatgttg gctggttatc gactgggctc agttgaactt
     3061 gagccctttt tgccgatgtt tattgacgca acagcgccag cacaccctgc ggcaactggt
     3121 tggcctgcgc caccatggcg gtgccggcct gctgcaggat ctgggtgcga gagagattgg
     3181 cagtctcgag tgcaaaatcg gcatcctgaa tgcgtgaacg tgatgcgctc agattttctg
     3241 ctgtggtttg caaattggat atggtggttt caaatctcga ctgcagagcg ccgaagctgg
     3301 cacgctgccc attgacggtg gccagagcgg aatctacggt tgacaatgag cgggttgcgg
     3361 cggcaacagt tgatacgtcc agtgtgctgg ctgcctgcaa ctggctacca gccgcagcag
     3421 cattgaaata gcctgtggcg gcggcggcct gtgcgcccgt agccactacg ccaaaggttt
     3481 tgtctgaatc aagggtcagc gcgccggtaa cataagctcc attagccgcc gccgctggag
     3541 cgccattgga tgcgacggct acagttgttc caactgttac gttgctacca gtcgctgata
     3601 tgttgccaat caatatgtca ttgccggtgg catttgtgac agttatgcca gtgcctgcag
     3661 aattgagttt tgcgctcagg ccagtctgtg atgttttgtc attgaaggcg gtgatgccag
     3721 cagcaagact gtcgggcgtg ttggcggggc tgcctacggt gaatgctatc gatactgcgg
     3781 ttgagttgtc cgacgttacc tgcaaggtgt aaccggcatt agcggcaaaa gtaccaagat
     3841 caatttcagt gcgcgcagtg gctgcaacac ctgtggtgcc cgactgtgcg ttgatagctg
     3901 cggccactgt ctttgcgctg tcgccagccg acacggggat ggtcgcactg ccactggcac
     3961 cattgacaat gacagtgcca ccgccggtga tccggctggt aatggctgcg gctgctccgg
     4021 ctgaagacgc cactgcgttt gccgtagaac ccgtaaccag gtcaccagcg ccacctgcat
     4081 tagctgacac taccgaacca atgcggttat tgccgtattt gttagtactg aagttcgcag
     4141 tcgtggcggt aatgctttgg ttcgcgttgg cacccacctg aaaggtagcg gagccaaaac
     4201 taccatctaa aagtttttgg ccgttgaatt cggtggattt tgcaattcgg tccagttcag
     4261 aggtgagttg tcccacctct gcgttgagcg cggcgcggtc gcttgccgag ttggtggcgt
     4321 tagccgactg cactgcaagt tctcgcaccc gttgcaggat cgtgccggcc gcacccagag
     4381 ccccctccgc aacctgcgcc agagaaatgc catcgtttgc attgcgtgcg gcttgtgtag
     4441 agccgcgaat ttgtgaggtc atgcggtctg caatggccag acctgctgca tcatccttgg
     4501 cgctgttgat ccgcaagcct gatgacaagc gttgcatgga agttgctagc gagccctgcg
     4561 actgactcag gttgcgctgc gctgtcatcg acgagatatt tgtattgatg gttgaggcca
     4621 ttgcgaaact cctaaaaaaa tagacaaacc ggtttcgccg ccgattgcaa agcctgtagt
     4681 ctccacaggc aatcagtaac gatgggtaaa ttgtggagag accgcgcctc cgtaaattgc
     4741 caataaaagc ggtaaaagcc cagctaattt gcagcaattt gcaaaacttt ataagggtta
     4801 accctaatat ggtggaattg aaggctaaaa atccattact tctcttttgt cgacccaaaa
     4861 acgtgtgtta actgggcgta ttgcgtgatt tggctaaagt ttctcgctgg tatcccgaaa
     4921 tcaattccaa cgggtcataa caaccccgtc gtccggcaag tgctttaggt ggcacaccgg
     4981 tcatggcgtt agcagcaatg ctgacggtga gatcggcatt tcgccggatt tagatggttc
     5041 ttttaggaga tatcacatga catccagtat caacagtaat atcaattcgt tgacagcaca
     5101 gcgcaacctc acttcatcgc agaactcgtt ggccacatcg atgacacgct tgtca
//
LOCUS       DAOSLL010000318         5151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14430_length_5151_cov_4.562402, whole genome shotgun sequence.
ACCESSION   DAOSLL010000318 DAOSLL010000000
VERSION     DAOSLL010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5151
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14430_length_5151_cov_4.562402"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="PLB25_13550"
     CDS             complement(<1..619)
                     /locus_tag="PLB25_13550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOE42637.1"
                     /translation="MTIQPAALSHLKVLDLSRVLAGPWCTQMLADLGADVIKVERPGA
                     GDDTRHWGPPFLKDAAGADTTEATYFTACNRNKRAITLDMAQPEGQALILRMAAQSDI
                     LVENFKVGGLRQYGLDYQSLKAENPRLIYCSVTGFGQTGPYAGRAGYDLMIQAMSGLM
                     SITGQADTEPGGGPMRIGVALIDILTGIYATSAILAALEVRHQTGE"
     gene            complement(659..841)
                     /locus_tag="PLB25_13555"
     CDS             complement(659..841)
                     /locus_tag="PLB25_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42638.1"
                     /translation="MQRLRFTLTDAGWYLAFQRRTPTLIGLWEFWPVADFAKRSALRP
                     LATSPTASQPNETDTD"
     gene            complement(1431..2627)
                     /locus_tag="PLB25_13560"
     CDS             complement(1431..2627)
                     /locus_tag="PLB25_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOE42639.1"
                     /translation="MAHAAFNWQDPFLLEQQLTDDERMIRDATSAYCQDKLLVRVVEA
                     FRKEQTDVSIFREMGELGLLGPTIPESYGGPGLNYVAYGLIAREVERVDSGYRSMMSV
                     QSSLVMLPIFEFGNEAAKQKYLPRLATGEWIGCFGLTEPDHGSDPGSMLSRAVKVAGG
                     YRLSGNKMWISNSPIADVFVVWAKEVSESGAVGPIRGFVLEKGAAGLSAPAIHGKVGL
                     RASITGEIVMDGVFCPEENAFPEVRGLKGPFTCLNSARYGIAWGALGAAEDCWLRARQ
                     YVLERQQFGRPLAANQLIQKKLADMQTEISLGLQGCLRLGRMKDEGIASVEITSILKR
                     NSCGKALDIARLARDMMGGNGISDEFGVARHLVNLEVVNTYEGTHDIHALILGRAQTG
                     IAAFGN"
     gene            3262..4161
                     /locus_tag="PLB25_13565"
     CDS             3262..4161
                     /locus_tag="PLB25_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOE42640.1"
                     /translation="MRRKIPSLQALACFEAAARHQSYTRAAQELALTQGAVSRQISTL
                     EAFVGVALFRRTRHGVTLTESGADYARQVAPRLAALEQDTLDVMSSQGRAQILKLAAV
                     PTFATRWLIPRLPLLKEQHPDITVHIETRTRPFMFADTPFDAALFAGTPAQLVNWAGT
                     QTTKLLDEDVVPVCHPKWLGAHTSLSPQVIATLPLLQQSTRTMAWRQWFEAQGVVAPQ
                     ALVGPRFELFSMTAAAASCGMGVALVPKLLVGAELAGGELVVACDQALPERRAYYLVQ
                     PEGLDNTVSRIFLDWLLSMTRPN"
     gene            4268..4756
                     /locus_tag="PLB25_13570"
     CDS             4268..4756
                     /locus_tag="PLB25_13570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HOE42641.1"
                     /translation="MPIITPQNDFDAEDSEALEFRRLSREEAQALREIQPPISPWRVI
                     RLQVVVGFLVSLLAWLFTMNLAVVWSAAYGALVVVIPAMLFARGLMSQFSSINAMTAG
                     FGFFVWEAAKIAVSVAMLFAAPKLVAGLDWLVMLIGLVVTLKVYWVALLMRPKLKLMS
                     KS"
     gene            4759..>5151
                     /locus_tag="PLB25_13575"
     CDS             4759..>5151
                     /locus_tag="PLB25_13575"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HOE42642.1"
                     /translation="MSAEANAPSASDYIVHHLTHWQNKTQTEIVDFSVFNIDSLFFSI
                     LLGVVGSFFLWKAASSATAGVPGRFQAAVEILMEMVDGQAKSIVHNATSRKLVAPLAL
                     TVFVWIFLLNAMDLLPLDLLPRIWEGIYS"
BASE COUNT         1061 a         1480 c         1478 g         1132 t
ORIGIN      
        1 cctcgccggt ctggtggcgc acttcaaggg ccgccagtat cgcgctggtg gcgtaaatgc
       61 cggtcaggat gtcgatcagc gccacgccga tgcgcattgg gccaccgccg ggctctgtgt
      121 cagcctggcc ggtgatactc atcagcccgc tcatcgcctg gatcatcagg tcataaccgg
      181 cgcgtccggc atacgggcca gtttgaccaa agccggtgac cgagcaatag atcaggcgag
      241 ggttttccgc cttcaggctc tggtagtcca gcccatactg gcgcagtcca ccgactttga
      301 agttctccac cagaatgtca ctttgcgctg ccattctgag gatcagcgcc tgaccctctg
      361 gctgggccat gtccagggta atggcgcgct tgttgcggtt gcaggcggtg aaataggtgg
      421 cctcggtggt atccgcaccg gcggcatctt tcaagaaggg cggcccccag tggcgggtgt
      481 cgtcaccggc accggggcgt tcaaccttga tcacgtcggc ccccagatca gccagcatct
      541 gggtgcacca ggggccagcc aacacgcggg acaggtcgag aactttgagg tgggagaggg
      601 cggctggctg gattgtcatg gtggtgtggt taatttttga cgggtcgaat tcagggggtc
      661 agtcggtgtc ggtctcgttg ggttggctgg ccgtggggct tgtggccagt ggcctcaacg
      721 cgctgcgctt ggcgaagtcg gccaccggcc aaaactccca taacccgatc agcgttggtg
      781 tgcgccgctg aaaagccaaa taccaaccgg catcagtcaa ggtgaaacga aggcgctgca
      841 caggtaactc cggtgacggt aggttgaatt cctgggcgca cccaaaaact ccgcaaacaa
      901 aaataactgg aatctgacct tttttaacat tgctgcgccc atgcgtccat gagtgcgtca
      961 aatgcctcgg ggttacccac atagtgaagc agaccgtggt cggcggcaag ccacttttgc
     1021 ttgctaccga gccatatgtg tccaactggt gcaatgtccc gcggattgtt cagggttccg
     1081 ggcttgatac tgagaatttg gtcttgcccc atcaccttgt gaaagaggcg tgtgccgcag
     1141 tcagggcaaa actgacacga catctgtttt cccgagggca tttggcgaat ccactccttg
     1201 atggggccgc cactgatctc gactactggc tgcttgatcc acaaagccat cccaaacgca
     1261 ctacccgact gtcgctggca ttcggtgcaa tggcatgcaa acatcgttag cgatgccccg
     1321 gtaacacggt aacgaatcga tgcgcactgg cactgtcctt caaatacttc ctgactcatg
     1381 gccaacgcct gtcaatgcat gccagccgac gcagcaccga gcatgggtgt ctagtttcca
     1441 aacgcggcaa ttccggtctg tgcccgcccc agaatcaggg cgtggatgtc gtgcgtgcct
     1501 tcataagtgt tcaccacttc cagattcacc agatgccggg cgacgccgaa ttcatcgctg
     1561 ataccgttgc cacccatcat gtcgcgcgcc aggcgggcaa tgtcaagcgc cttgccgcag
     1621 gagttgcgct tcaggatgga cgtgatttcg accgaggcga tgccttcgtc tttcatgcgt
     1681 cccaggcgca aacagccttg caagcccagc gaaatttcgg tctgcatgtc agccagtttc
     1741 ttctggatca actggttggc cgccagcggg cggccaaatt gctggcgctc caacacgtac
     1801 tggcgtgcgc gcaaccagca gtcttctgcg gcacccaacg caccccaggc gatgccgtag
     1861 cgggcgctgt tgaggcaggt gaacgggcct ttgaggccgc gcacttcggg aaaagcgttt
     1921 tcctccgggc aaaacacccc atccatgacg atttcaccgg tgatgctggc gcgcagaccg
     1981 accttgccgt gaatggccgg ggcgctcaat ccggccgcac ctttttccag cacaaaacca
     2041 cgaatcgggc caaccgcgcc ggattcggac acctccttgg cccagaccac aaacacatcc
     2101 gcaatcgggc tgttggaaat ccacatcttg ttgccgctga gcctgtaacc accggccacc
     2161 ttgacggcac ggctgagcat gctgccgggg tcggagccgt ggtcgggctc ggtcagccca
     2221 aagcagccaa tccactcacc agtggccagc ctgggcaggt atttctgttt cgcggcttcg
     2281 ttgccaaact caaaaatcgg cagcatcacc agcgagcttt gcacgctcat catggagcga
     2341 tacccgctgt ccacccgctc gacctcgcgg gcaatcaggc cgtacgcgac gtaattcagg
     2401 ccagggccac cgtaactttc cggaatcgtc ggacccagca gcccgagttc gcccatctcc
     2461 cgaaagatgc tgacatcggt ttgctctttg cgaaaggctt ccaccacacg caccagcagc
     2521 ttgtcctggc agtaggcgct ggttgcgtcc ctgatcatgc gctcgtcgtc ggtgagttgc
     2581 tgctcaagta aaaaggggtc ttgccagttg aatgctgcgt gcgccatggg ggtctttcgg
     2641 tcagtgctga gatgactgca tgttagcgcc gcaggccacg ctgcacaaac gacttttttg
     2701 catcaaggca tgcgcctaag gaatatccaa ggaatgaacc caagactata gttgttaaga
     2761 agccctgaca agaatcggct ctatcgccct atatcaatgc ctgtaaagct attgttaata
     2821 tagcgtagct tgctttgcca aaaaatgaaa tgtgcaacat tcacactcca agcattcaat
     2881 gtcaaagttc gcgtccatgg cgcccgtcgg acctatggga tcgttggcaa gaatcgaccc
     2941 cagcgtggcc agcttgtgcc gaacttgcag gccgacagcc gcccacccat cggtcaataa
     3001 ggacgcgaac aaggcgccgc aacggtcggc gcgaagccga tgccccatcg accgatccgt
     3061 aattgggatc tcggcaaagg caaacatcag caaccaagct acccaataga tgaccagaag
     3121 acatgtgcca tcagcgccag ccttgggtca atgcgcgcat catttttgga cttggcgctt
     3181 gtcatcaagg cgctgttagc tctaattgtt gaagcgatct gctttttgcg gtgcgcagaa
     3241 catgggtaca ttgcatgcat catgcgcaga aaaatcccgt ccctccaagc gctggcgtgt
     3301 tttgaagctg cggcacgcca ccaaagctat acccgcgcgg cccaggaact tgcactgacc
     3361 cagggtgcgg tttcgcgcca gatcagcacg ctggaagcgt tcgttggcgt ggcgctgttt
     3421 cgccggactc gccacggtgt cacactgacc gaaagcgggg cagactatgc acgtcaggtc
     3481 gcgcctcgcc tggctgcgct ggagcaggac acactcgatg tcatgagcag ccagggccgg
     3541 gcgcagatac tgaagctggc ggcagtcccg accttcgcca cccgttggct gattccacgc
     3601 ctgccactgc tgaaagagca acaccctgac atcaccgttc acattgaaac gcgtactcgc
     3661 cccttcatgt ttgccgatac cccctttgat gccgcgctgt ttgctggcac ccccgcgcaa
     3721 ctggtcaact gggccggcac ccagactacc aaactgctgg atgaagatgt ggtgccggtg
     3781 tgtcacccga agtggcttgg cgcccacacc agtctgtcac ctcaagtcat tgcgacgctg
     3841 ccgttgctgc agcaaagcac ccgcacaatg gcttggcggc agtggttcga ggcgcagggc
     3901 gtggtcgcac cgcaggcgtt ggtgggcccg cgttttgaac tgttttcgat gacggcagcc
     3961 gctgcaagtt gcggcatggg tgtggcactg gtgcccaaat tgctggttgg cgccgaactc
     4021 gcgggtggtg aactggtggt tgcttgcgac caagccttgc cagaacgcag ggcctactat
     4081 ctggtgcaac ctgaaggcct ggacaacacg gtgagcagaa tcttcctcga ctggctgcta
     4141 tcaatgacgc gccctaactg aggcgcaatc agaacaggag ccgcccggca gcctgtttac
     4201 agcatctttc gacctataat taaggccccg aagacagcca aagcgtgtaa cctgcgtgta
     4261 atttgagatg ccgataatta cgccacaaaa tgactttgat gctgaagatt ctgaagcatt
     4321 ggagttcagg cggttaagcc gggaagaggc tcaagcgttg cgggaaatcc agccacccat
     4381 ttcgccttgg cgagtgatac ggttacaggt ggtggtaggg tttctggtgt cgttgttggc
     4441 ctggcttttc accatgaact tggcagtggt gtggtctgca gcttatgggg cattggtggt
     4501 ggtcattccg gcaatgctgt ttgcccgggg cttgatgagt cagttctcgt cgatcaacgc
     4561 aatgactgcc gggtttggtt tttttgtatg ggaagccgca aaaattgcgg tctcggtcgc
     4621 gatgcttttt gcggcaccaa agttggttgc tgggctggac tggttggtca tgttgatcgg
     4681 tttggtggta accctgaagg tatattgggt ggcgctgttg atgcgtccaa aattgaaact
     4741 gatgagtaag agttaaccat gtccgcagaa gcaaacgcac cgtcagccag tgactacatc
     4801 gttcatcacc tgacccattg gcagaataaa acccagaccg aaatcgttga tttttctgtc
     4861 ttcaacatcg attccctgtt tttctccatt cttctgggcg ttgttggcag tttttttctc
     4921 tggaaggcgg ccagtagcgc cacagcggga gtgccaggac gcttccaggc ggccgttgaa
     4981 atactcatgg aaatggttga cggccaagcc aaatcgattg ttcacaatgc caccagtcgc
     5041 aaattggttg caccgctggc gctgacggtg tttgtctgga tttttctgct caatgccatg
     5101 gacttgttgc cgctggacct gttgcccaga atttgggaag gtatttacag c
//
LOCUS       DAOSLL010000319         5143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14457_length_5143_cov_5.035377, whole genome shotgun sequence.
ACCESSION   DAOSLL010000319 DAOSLL010000000
VERSION     DAOSLL010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14457_length_5143_cov_5.035377"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="PLB25_13580"
     CDS             complement(<1..215)
                     /locus_tag="PLB25_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HOE42643.1"
                     /translation="MTVQQHFLSTRLGPTPARWCQAFPAGQVHTLQELRLQVAKNWLG
                     LLWLTSDDPRWPDHLRAVRQQQAGLPVV"
     gene            complement(225..1643)
                     /locus_tag="PLB25_13585"
     CDS             complement(225..1643)
                     /locus_tag="PLB25_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HOE42644.1"
                     /translation="MMAWFSAKAFERSGAAIKRASRSSEATFGPLVKRLTPIETNDNL
                     NWAGDADWARLQQDPLRARGLLRVVAGALLVMLVWAAFAPIDEITRGEARVVPTTQVQ
                     IVQSVDGGVVEALLVKEGQIVEAGQLLLRVDPTRFVSNMLESRAGQTGLQAKALRLEA
                     LTRGTPFTPPAALMKEAPEVVAQELRLYESRRAEISAQTSIYQSQLVQRQQELNEVRA
                     RRDQAERSLELMTKELNATRPMVATGAVSEVEVLRLEREVARLRGDREQATAQISRVQ
                     SAIQEASSKINEVQLTSRNQMSAELSETMSKLASLSQGGKALEDKVKHADIKSPVRGT
                     VKRLLVNTVGAVVQPGKEVVEVVPLDDALILEVQIAPKDIGFLRAGQGAMVKFSAYDF
                     SIYGGLAAEVFLIGADSVLDEKGNAFYHVRVKTKQTSLGPNLPIIPGMVAQVDIMTGK
                     KTVLTYLLKPVLRAKANALTER"
     gene            complement(1640..3799)
                     /locus_tag="PLB25_13590"
     CDS             complement(1640..3799)
                     /locus_tag="PLB25_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HOE42645.1"
                     /translation="MSNPVTANEVVRNKDQPDDLLACLQIIARAHGQSATYDALMSGL
                     PAENARLTPGLFARAAKRAHLSSRLAQCRLDQLTSSLFPVILLLKGERACVLLEFDDS
                     VSQATIIYPELVDAPVTVPLDKLLLEYTGDAIYARPTQRYDARTPTVRDGRHDHWFWG
                     VIAESRPLYQDVLVAAFLANLFALGMPLFTMNVYDRVVPNQAIDTLWVLALGLVLMMV
                     SDLVLRTMRGRFVDLASSRADVKLSAFIMERVLGMRMEQRPTSAGSFASNLRAFESVR
                     DFIGSATVVAFIDLPFAVIFIVAIGWISWAMLIPLFFGGAIMVLYALAVQGRMHELAE
                     TTYRAGAQRNATLIEGLVGFETIKAMAAESPIQRKWEKSAALLARVGAQLRLLSTTAS
                     NTSAFVQQLINLVIVVIGVYLIYDRQLSMGGLIACTMLAGRAMAPVSQVAGLLVQYHT
                     AATALTSLNEMMKREVERPQETSFISRGRFSGAIDFREVSFSYPGQTASSLRNISLRI
                     KAGEKVAILGRIGSGKTTLEKLILGLYRPSEGAVLVDSIDLRQLDPAELRHQIGYVQQ
                     DIILFYGTLRENITMGAPLASDAEVVKAAEVGGILGLVNQHPQGFDMLVGERGESLSG
                     GQRQGVAIARAVINNPAILLLDEPTSSMDYSSEDDIKRRLSEFAQDKTVVLISHRTSL
                     LDLVDRIIVMDGGKIVADGPKEQVITALKQGRIGKAA"
     gene            complement(4001..4417)
                     /locus_tag="PLB25_13595"
     CDS             complement(4001..4417)
                     /locus_tag="PLB25_13595"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HOE42646.1"
                     /translation="MNTIHVDVVSAEESIFSGEARFVALPGEAGELGIYPRHTPLITR
                     IKAGSVRIEMADGGEEFVFVAGGILEVQPNCVTVLSDTAIRGKDLDDEKANEARLAAE
                     EAVKNAKSDLDLAKASSELAIMAAQISALRKYRGKK"
     gene            complement(4484..>5143)
                     /locus_tag="PLB25_13600"
     CDS             complement(4484..>5143)
                     /locus_tag="PLB25_13600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HOE42647.1"
                     /translation="VDNIYRFTLAGTEVSALLGRMPSAVGYQPTLAEEMGRLQERITS
                     TKVGSITSIQAVYVPADDLTDPSPATTFAHLDSTVVLSRDIASLGIYPAVDPLDSTSR
                     QLDPLVVGLDHYETARAVQGTLQRYKELRDIIAILGMDELAPEDKLTVARARKIQRFL
                     SQPFHVAEVFTGSPGKYVSLAETIRGFKMIVAGECDHLPEQAFYMVGTIDEAFEKAKK
                     I"
BASE COUNT         1132 a         1477 c         1439 g         1095 t
ORIGIN      
        1 accaccggca agccagcctg ctgttggcga actgcccgca ggtgatctgg ccagcgcggg
       61 tcatcgctgg tcagccacag caggcccagc caattcttgg ccacctgcaa ccgcaactct
      121 tgcagggtgt gcacttgtcc ggctggaaag gcctgacacc agcgcgccgg tgtcgggcct
      181 aagcgggtac tgagaaaatg ctgttgaacg gtcatggtgc ccacctatct ttcagtcagt
      241 gcgttggctt tggcccgcag taccggctta agcaggtacg tcagcacggt ttttttgcct
      301 gtcatgatgt cgacctgagc gaccatgcct ggaatgatgg gcaaattggg accaaggctg
      361 gtttgtttgg ttttcacccg cacatggtag aaagcattgc ctttttcgtc cagtaccgaa
      421 tctgcgccaa tcaggaaaac ctctgccgcc aggccaccgt aaatcgaaaa atcataggca
      481 ctgaacttga ccatggcccc ctggcctgcg cgcaaaaacc cgatgtcttt tggcgcaatc
      541 tgaacttcaa gaatcaaggc atcatcgagc ggcacaactt caaccacttc cttgccgggc
      601 tgcaccacgg cacccacagt atttaccagc agccgcttga cggtaccacg cacgggcgac
      661 ttgatgtcgg cgtgcttgac cttgtcttcc agcgccttgc caccttgcga caggctggca
      721 agcttgctca tggtctcaga cagttcggca ctcatctggt tgcggctagt gagctggact
      781 tcgttgattt tgctgctagc ttcctgaatg gcggactgaa cacgcgagat ttgggcggtg
      841 gcttgttcac ggtcgccgcg aagccgggcg acttcgcgct caaggcgcag cacctcaacc
      901 tctgacaccg ctccagtggc caccatgggt cgggtggcgt tgagttcttt ggtcatcagt
      961 tccaggctgc gctcagcttg atcgcggcgg gcacggactt cgttgagctc ttgctggcgc
     1021 tgaaccagtt ggctttggta gatggaggtc tgcgcgctga tttcggcgcg gcgtgactcg
     1081 tacaggcgca attcctgggc gaccacctcg ggtgcttctt tcatcaacgc ggccggggga
     1141 gtaaacgggg tgccgcgtgt caatgcctcc agacgcaatg ccttggcctg caagccggtt
     1201 tgtccggcac ggctttccag catgtttgag acaaaccgcg ttgggtcaac ccgtaatagc
     1261 agttggccgg cttcaacaat ctggccctct ttgaccagca aagcctcgac cacgccaccg
     1321 tcaacactct ggacgatttg aacctgggtg gtgggcacta cgcgggcctc accccgggtg
     1381 atttcgtcga tcggggcgaa cgctgcccac accagcatca ccagcaatgc gccagcgaca
     1441 acgcgcagca ggccacgtgc ccgcaagggg tcttgctgaa ggcgcgccca gtcggcatcg
     1501 ccagcccagt tcaggttgtc attggtttcg atcggggtga gccgcttgac cagtgggcca
     1561 aaagtcgctt cgcttgacct gctggcgcgc ttgattgctg cgccgctgcg ttcaaaagcc
     1621 tttgcactga accatgccat catgcagcct ttccaatacg cccctgcttg agagcagtga
     1681 tcacttgttc ttttggccca tcggcgacga ttttgccgcc gtccatgacg atgattcggt
     1741 caaccaggtc gagcaaagaa gtgcggtggc tgatcagtac gacggtcttg tcctgagcga
     1801 actccgaaag gcgacgcttg atgtcatctt cactggagta gtccatggac gaggttggct
     1861 cgtcaagcag caagatggcg gggttgttaa tgaccgcgcg tgcgatggca acaccctggc
     1921 gttgtccgcc ggagagggat tcgccacgct caccgaccag catatcaaag ccctggggat
     1981 gttggttgac caggccaagg atgccaccaa cttctgcggc cttgacgact tcagcgtcgg
     2041 acgcgagagg tgcccccata gtgatgtttt cacgcaaggt gccataaaaa agtatgatgt
     2101 cttgttgcac gtagccgatt tgatgacgca actctgccgg gtcaagctgt ctcaagtcga
     2161 tgctgtccac cagcaccgca ccttcactgg gccgatagag tccgaggatg agcttttcaa
     2221 gggtagtctt gccggaaccg atgcgcccga gaatggcaac cttttcgcct gccttgattc
     2281 tcaggctgat gttacgcagc gatgaggcgg tttgaccggg atagctaaat gacacctcac
     2341 gaaagtcaat ggcgcctgag aatcgtcccc ggctgataaa actggtttct tgcgggcgct
     2401 cgacctcgcg cttcatcatt tcattgagtg atgtgagtgc tgttgctgcc gtgtggtact
     2461 gcaccagcaa gcctgccacc tggctgacgg gggccatggc acggcctgcc aacatggtgc
     2521 aggcaatcaa cccccccatg ctgagttgac ggtcatagat caaataaaca ccgatcacca
     2581 caatcaccag attgatcagt tgctggacaa aggccgaagt gttgctggcc gtggtggaca
     2641 gcaaacgcag ttgtgcgccg acacgtgcca gcaaggctgc gcttttttcc catttgcgct
     2701 gaatgggcga ctcggctgcc atggctttga tggtttcgaa gccaaccaga ccttcaatca
     2761 aggtagcgtt gcgctgggca cccgctcgat aggtggtttc cgcaagctcg tgcatacgtc
     2821 cctgcaccgc caatgcatac agaaccatga tggctccgcc gaaaaacaag ggaatcagca
     2881 tggcccatga aatccagccg atggcgacta taaaaatgac cgcaaaaggc agatcgatga
     2941 aagccaccac tgtggcagag ccaataaaat ccctgaccga ctcaaaggca cggaggttgg
     3001 atgcaaagga ccccgcagaa gtagggcgtt gctccatacg catgccaagc acccgctcca
     3061 tgataaaggc ggacaatttg acatcggcgc gactgctggc gaggtcaaca aagcgtccac
     3121 gcatggttcg caacaccagg tcactgacca tcatcaacac cagacctaga gccagcaccc
     3181 ataaggtatc aatcgcctgg ttgggcacaa cgcgatcata aacattcatg gtaaaaagcg
     3241 gcatgccaag cgcaaagagg ttagccagaa acgccgctac aagcacatcc tgatagagcg
     3301 gacgactctc ggcaatgaca ccccagaacc aatggtcgtg ccgcccgtcg cgaaccgttg
     3361 gtgtgcgcgc gtcgtagcgc tgcgtgggac gtgcataaat ggcatcgccg gtgtactcca
     3421 gcaatagctt gtccagtgga accgtcaccg gggcatcgac gagttccggg taaatgatgg
     3481 tggcctggga aacgctgtcg tcgaactcca gcagcacgca agcgcgttca cctttgagca
     3541 gcaagatgac cggaaacaaa ctgctcgtga gttgatcaag ccgacactgg gccaaacgac
     3601 tgctcagatg agcgcgcttg gccgctctgg caaacagacc gggtgtcagc cgggcattct
     3661 ctgcgggcaa acccgacatc aatgcatcgt aggtggcgga ttgaccgtgc gccctggcga
     3721 tgatttgcaa gcaagccaac aggtcatctg gctgatcctt gttgcggaca acctcatttg
     3781 cggtgaccgg attggacatc tgcttcctga ctttgtattg atctaaaacc aaaccagttt
     3841 agcaaatcca atcagatgta aacaaacaat ctcggcaaaa ctcgtgtgcc aagtcccgcc
     3901 cgaaagtgcg tcgtcaccaa taaaaaggcc tgatcttttt gggatcaggc cttctgagac
     3961 agtggcaaaa aagcaggggt ttgtctgcta ccaagatgcc ctatttcttc ccgcggtact
     4021 ttcgcaaagc actgatttgc gctgccatga ttgccaactc ggacgaggct ttagccaaat
     4081 ccagatccga cttggcattc ttgaccgcct cttcagccgc caacctggct tcatttgcct
     4141 tttcgtcgtc caggtcttta ccgcgtatgg cggtgtcgga cagcacggta acgcaatttg
     4201 gttgaacttc aagtatgccg ccggccacaa atacaaactc ttcgccaccg tcagccatct
     4261 caatgcgcac tgaaccggct ttgatacggg taatcagcgg tgtatggcgc ggatatatac
     4321 cgagttcgcc tgcctcgcca ggcagcgcga caaagcgcgc ctcacctgag aaaattgact
     4381 cttcggcact gaccacatca acgtggatgg tgttcatggt gttcctaatc taatggggac
     4441 agagtttgat gccatgcgac gccaccctgt cccgccaggg ttgttagatc ttcttggctt
     4501 tctcgaaggc ttcatcgatc gtgccaacca tgtaaaaggc ctgctctggc aggtggtcac
     4561 actcgcccgc cacaatcatc ttgaacccgc ggatggtttc agcaagcgac acgtatttgc
     4621 caggcgagcc ggtgaacact tcagccacat ggaacggctg ggacagaaag cgctggatct
     4681 tgcgggcacg tgccacagtc agcttgtctt ccggggccaa ttcgtccatc cccagaatgg
     4741 caatgatatc gcgcagttct ttatagcgtt gaagggtgcc ttgcacggca cgggcagttt
     4801 cgtagtggtc cagacccact accagcggat caagctggcg actggtggag tcgagcgggt
     4861 cgacggctgg gtaaataccg agcgatgcaa tgtcccgact tagcacaact gtggaatcca
     4921 agtgggcaaa ggtagtggca ggagacgggt cggtcaaatc atcggctggc acgtacaccg
     4981 cctgaataga ggtgattgaa ccgaccttgg tcgaggtgat ccgctcttgc aggcgaccca
     5041 tttcctcagc caacgtcggc tggtaaccca cagcggaagg catgcggccc agcaatgctg
     5101 acacttcagt gccggccagc gtgaaacggt agatgttgtc aac
//
LOCUS       DAOSLL010000320         5127 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14513_length_5127_cov_4.167587, whole genome shotgun sequence.
ACCESSION   DAOSLL010000320 DAOSLL010000000
VERSION     DAOSLL010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5127
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14513_length_5127_cov_4.167587"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /gene="carB"
                     /locus_tag="PLB25_13605"
     CDS             <1..819
                     /gene="carB"
                     /locus_tag="PLB25_13605"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836975.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HOE42648.1"
                     /translation="AQALNVVGLMNVQFAIQHIDGRDVIYVLEVNPRASRTVPFVSKA
                     TGVQLAKVAARCMVGQTLAAQGITREVTPPYFSVKEAVFPFVKFPGVDTILGPEMKST
                     GEVMGVGKTFGEAFVKSQLGAGTKLPRPLLPDGSSSGKVFVSVKGSDKPRAVEVARAL
                     VDMGFVVVATKGTAAAISAAGVPCGVVYKVTEGRPHIVDMMKNNEIILVINTVEERRN
                     AIADSRQIRTSALLARVTTFTTIAGAEAAVEGMRHMDQLGVISLQEMHAQLLAA"
     gene            complement(1399..1938)
                     /locus_tag="PLB25_13610"
     CDS             complement(1399..1938)
                     /locus_tag="PLB25_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOE42649.1"
                     /translation="MPVVIVLASGRGERFAASGGVTHKLQAQLIGKTVLQHTLDAVWG
                     SGLAWHLEDEGHTGMGDSIAAAVQKTSQANGWLILPADLPLIQSSSIMAVAMALVSHE
                     VVVPVYQGQRGHPVGFSVVCRQALLCLRGDRGAATVVRSHVTTELKLDDKGCVTDIDT
                     VEDLNVAERLLRHAGPATQ"
     gene            complement(2005..3444)
                     /locus_tag="PLB25_13615"
     CDS             complement(2005..3444)
                     /locus_tag="PLB25_13615"
                     /inference="COORDINATES: protein motif:HMM:NF012271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOE42650.1"
                     /translation="MNRKLLFQIACAVALTSAFSNRVQAAGPFVVFNGVPVKYAGAGA
                     IVLNYDQGSLGSRTKAQADTIVTQAVSLWTNVATSTVTISRGSDLPADVNTSNYLTYF
                     GTLNASVSGDGLNPVVYDSDGSIIDLFFGVGAKNSLLGFAGSAWSYSYPDFIGTFTDG
                     RAVINGWRSVTDDTMKAVIAHEIGHLIGLDHTQIDSSQGLTSAYPSNYPLMYPIAYRS
                     FVSLHEDDVAAVSALYPDAALGGTYGTLTGTFVQANGTPVRGANLWAQETVSHQLFSV
                     VSDYLDQGTGYFKLILPAGAYNLHAQAVKTSFTGASGVGPHATNSADFSFQAPLYVGG
                     VAMTPLTLGNGTPVKISIVAGCAATATFRMDGTGDIGGNCPIVPGVPTAVSVKAGAGS
                     AIVSFTPPLNAGSTTSYTVNCTAAGQPTGTQSGTTSPIRVDGLTPGATYSCTVYASNA
                     AGTSATTAPVPVIVGKKIDLTPILMLLLD"
     gene            complement(3441..4022)
                     /locus_tag="PLB25_13620"
     CDS             complement(3441..4022)
                     /locus_tag="PLB25_13620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42651.1"
                     /translation="MNYKSVLTSLICLSLSLLTQAATVLPMYLDEIVADAAVAFEGVC
                     KDTRTELDAQTGLVATYTTFDIIDLLKGSAKATHTIKQLGGNTGTVAYKVGGTPSFVV
                     GTRYVVFLYGASSAGFSSPVGLTQGRFMIRNGPGGDELSNGRDFKEMLRPYPDRLLPP
                     GAKKAVQQATGELGHLDLAEFKQIVRQPGSTAK"
     gene            4465..>5127
                     /locus_tag="PLB25_13625"
     CDS             4465..>5127
                     /locus_tag="PLB25_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE42652.1"
                     /translation="MTLKFAFRLSALVCAALSSTALLAEPAVVFDMGGKFDKSFNEAA
                     YNGMEKWKKESGKNYLEFEVTNESQREQALRRMAEKGARPIIGIGFGQASAIEKLAKE
                     FPKLQFAIIDMVVNQPNVESVVFKEQEGSFLVGAMAAMASKTGKVGFVGGMDIPLIRK
                     FQCGYEQGVKYVNPKAEVFANMTGTTGAAWNDPARGGELAKAQFAKGADVVFAAAGGT
                     GIG"
BASE COUNT         1115 a         1377 c         1499 g         1136 t
ORIGIN      
        1 gcgcaggcgc tcaacgtcgt cggcctgatg aacgtgcagt ttgcgattca gcacattgac
       61 gggcgggatg tcatctacgt gcttgaggtg aatccacgtg cttcgcgtac cgtgccgttt
      121 gtgtccaagg ccaccggcgt gcaactggcc aaggtggcag cgcgctgcat ggtcgggcaa
      181 acactggcag cccaaggcat cacgcgtgag gtgacaccgc cgtatttcag cgtcaaagag
      241 gcggtgttcc cgtttgtcaa gttccccggt gtcgatacca ttttgggtcc cgagatgaaa
      301 tccaccggcg aggtcatggg tgttggcaag actttcggcg aagcatttgt caagtcgcaa
      361 ttgggcgctg gcaccaagct gccccggccg ttgttgcctg atggttcatc cagcggcaag
      421 gtgtttgtgt cggtcaaggg cagcgacaaa ccgcgtgccg tcgaggtcgc ccgggccttg
      481 gtcgatatgg gctttgtcgt ggtggccacc aaaggtaccg ccgcagccat cagcgcagcg
      541 ggtgttccct gcggtgtggt gtacaaagtc accgaaggcc gaccacacat tgtggacatg
      601 atgaaaaaca acgagatcat tttggtgatc aataccgtcg aggagcgtcg caacgcgatt
      661 gctgactcgc gccagattcg tacctccgcg ctgctggcac gggtgaccac ctttaccacc
      721 atcgctggtg ccgaggcagc agtcgaaggc atgcgtcaca tggaccagct tggggtgatt
      781 tcgctgcagg aaatgcacgc ccagttgctg gcagcctgat tgagcggatg tcgggccgca
      841 gccagttgaa aaagccacct ggcatggaat cagtccagcc aggccctgag tcggcatcct
      901 gactgttgaa cacccggcgc cattgccgcc accggcagca caggcgcatt tggtagcaga
      961 ccctggagct gctcgccatt cgggcccaat gtggctggtt cggcttgact gcgctaccct
     1021 gattcgggct ctgacaccat atcctgcggg ccatcagcgg cgctatggtg cgtgccaggt
     1081 ggaatcagga ttttgagata agaatggcct gcagcgctta tgcagcatgc gctggcagct
     1141 attgaaatga taacagtttg gcattcggca gtggcaagtt accgctgtca ttcggtcggt
     1201 ttttagtgag ccgcatgatt gccattgccg ccttcaaatg tcatttgggg ccttcagact
     1261 gaacaggtct gaatctgaca ctgttcaggt ttcagcgtca gaagcagatt ttttccttgg
     1321 gttggggcgg atttgtggct tggcactgcg ctcagtcacc ccctgatgtt ctggcgttgc
     1381 gaaccccaaa gacggcgact attgtgttgc aggtcctgca tgtctcaaca gacgctcggc
     1441 gacattcaag tcttctacgg tgtcaatatc agtcacgcaa cccttatcgt ccaatttcaa
     1501 ttccgtggtg acatgcgaac ggacaacggt agccgcccca cgatctcccc gcaaacacaa
     1561 caatgcctgg cgacacacca cggagaaccc caccggatgg ccacgttgcc cttggtaaac
     1621 aggaacaacc acttcatggc tcaccagtgc cattgcaact gccatgatgc tgctgctttg
     1681 aatcagcggc agatccgccg gcaggatcag ccatccattc gcttgcgatg ttttttgcac
     1741 tgcggctgcg atcgagtcgc ccatgccggt gtggccttca tcttcaagat gccacgcaag
     1801 accgctgccc cacactgcgt ccagtgtgtg ctgcagaact gttttgccaa tcagttgcgc
     1861 ttgcaatttg tgagtgactc cccccgacgc agcaaaacgc tcaccgcgcc ctgaggccaa
     1921 aacaatgacg acgggcatgt tcgaagcagc acctgttggt tcagatgttc tgccgacggt
     1981 cggttgaatt gcctgcaacc cagttcaatc cagcagcaac atcaatattg gggtcagatc
     2041 gattttttta cccacgatca ccggaaccgg cgcggtggtc gcgctggttc ctgccgcgtt
     2101 gcttgcgtag accgtgcaag agtaggttgc gcctggtgta aggccatcaa cccggatggg
     2161 cgatgttgtt cctgattgcg ttcccgtggg ctggcctgct gccgtgcagt tgaccgtgta
     2221 gcttgtggtg ctaccggcat tcagcggtgg cgtaaaagac acaattgcgc tgccggcacc
     2281 agccttgaca ctgacggctg tgggcacccc gggaacaatt ggacagttcc cgccgatgtc
     2341 tccggtgcca tccatcctga atgttgccgt ggcagcacac cctgcaacaa ttgaaatctt
     2401 gaccggagtc ccgttgccca aggtcaaggg tgtcatcgcc accccgccaa cgtaaagggg
     2461 tgcctggaaa gaaaagtctg ccgagtttgt ggcgtgggga cccacgcctg aggcacccgt
     2521 aaaggaagtt tttacggctt gcgcgtgcag gttgtaagca cctgcaggca ggatcaactt
     2581 gaaataaccg gtgccttgat cgagatagtc agacacaacg ctaaacagtt gatgactgac
     2641 ggtttcctgt gcccaaagat ttgcaccgcg aaccggagtg ccattggctt gcacaaatgt
     2701 gccagtcaat gtgccgtaag tgccacccag tgcggcatcg ggataaaggg cactgacagc
     2761 cgcgacatcg tcttcatgca aactgacgaa actgcgatag gcgatgggat acatcaacgg
     2821 atagttggac gggtaagctg atgtcaagcc ctgggaactg tcgatttggg tgtggtccag
     2881 gccgatcagg tgtccgatct catgcgctat gaccgccttc atggtgtcgt cggtgaccga
     2941 cctccatccg ttgatgacgg cgcggccatc agtgaaagtg ccgatgaagt cgggatagga
     3001 gtacgaccag gccgaaccag caaagcctaa cagactgttt tttgccccaa cgccaaaaaa
     3061 cagatcaatg attgatccgt ctgaatcgta aacaaccggg ttcagaccat cgccggaaac
     3121 actggcgttc agcgtaccga aataggtaag gtagttggaa gtattcacgt cagccggcaa
     3181 atcgcttccg cgtgaaatcg tcacggttga tgtcgccaca ttggtccaaa gtgacacggc
     3241 ttgtgtcacg atggtatccg cctgtgcttt cgttcggctc ccaagactgc cttggtcgta
     3301 gttcaggaca atggcccccg cacctgcata cttgacggga accccgttga aaacgacaaa
     3361 gggtccggca gcctgtaccc gattactgaa agcgcttgtc aaagcgacgg cgcaggcgat
     3421 ctgaaacagc agtttgcggt tcattttgcc gtgctgccag gctgtctgac gatctgcttg
     3481 aactcagcaa ggtccaaatg accaagttct cctgtagctt gctgaacagc ctttttggca
     3541 ccaggtggca gcaaacggtc aggatacggt cgcaacattt ccttgaaatc acgaccattg
     3601 gaaagctcgt caccaccggg gccgttgcgg atcatgaaac ggccttgagt gaggccgacc
     3661 gggctggaaa accctgcact ggatgcaccg taaagaaaca cgacgtaacg ggttcccacg
     3721 acaaaagaag gcgtaccacc caccttgtag gcgaccgtgc cggtgtttcc gccaagttgc
     3781 ttgatggtgt gggtggcctt tgctgagcct ttgagcaaat cgatgatgtc aaacgttgta
     3841 taggtggcca ccagaccggt ctgggcatca agctcagttc gggtatcttt gcatacaccc
     3901 tcaaaagcca ccgctgcgtc agccacaatt tcatcaaggt acatgggcag cacggttgcg
     3961 gcctgggtta aaagcgacaa gctcaggcaa ataagtgatg tcaaaacaga tttgtagttc
     4021 atggcggcgg gtccttgaga actatgagtt tgagaactat ggaaagcttg tttggatcga
     4081 taacagccaa gttttcaagc acgggagtcc aagtatcgca ccttggaaaa aagaattggc
     4141 cccggtattt tgaacacccg tgtctctgag tacgcgccgc ctgggcgcag gccactgcct
     4201 ggttggcacc ttgcccgctg gggcaggtgt tgaagtcgat tcccggcacg gtttctccgg
     4261 gggagggcgg aaatctggtg ggcacggacc gcacagttca aattggcgga gccgacgacc
     4321 agaatcatgc acgttggctt gaagtgtgtg gaccgcagca tatgtgtcct ttcgtttagc
     4381 gcactgtatt cgtggcatag tcatcgctgg cctggcggtg agcctcattg ccaggatttg
     4441 tccaaccttt gaaagggatc tgtcatgacg ttgaaattcg cttttcgttt gagtgcgctg
     4501 gtttgcgccg ctttgtccag cacagcgctt ctggctgagc ccgctgtggt gtttgacatg
     4561 ggcggcaaat ttgacaagtc gttcaacgaa gcggcctaca acggcatgga aaagtggaag
     4621 aaagaatccg gcaagaatta cctggagttt gaagtcacca acgagtcaca gcgcgagcag
     4681 gccttgcgcc ggatggctga aaaaggggcc aggccaatca ttggcatcgg atttggccag
     4741 gcatcggcca ttgaaaaact ggccaaggag ttccccaagc ttcagttcgc cattatcgac
     4801 atggtggtca atcagcccaa tgtcgagtcg gtggtgttca aagagcaaga gggcagcttt
     4861 ttggttggtg ccatggccgc gatggccagc aaaaccggca aagttggctt tgttggtggc
     4921 atggacattc cgctgattcg caagttccag tgtggctatg agcagggtgt caaatatgtc
     4981 aacccgaagg ctgaggtgtt tgccaacatg accggcacga ccggcgccgc ctggaatgac
     5041 ccggcacgcg gtggcgagtt ggccaaggcc cagtttgcca agggtgctga tgtggtgttt
     5101 gcggcagcag ggggtaccgg tattggc
//
LOCUS       DAOSLL010000321         5121 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14532_length_5121_cov_5.318792, whole genome shotgun sequence.
ACCESSION   DAOSLL010000321 DAOSLL010000000
VERSION     DAOSLL010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14532_length_5121_cov_5.318792"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..358
                     /locus_tag="PLB25_13630"
     CDS             <1..358
                     /locus_tag="PLB25_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HOE42653.1"
                     /translation="NTGLPSSVGVVQAVFNPDNLDFVIAYSLVGGVFAALVFAIAVVS
                     IPMILDRDTDAISAAITSLEVVVNNTAVMLLWGAVIVLLVAASLLLPWALGLLAVGPL
                     LGHASWHAYRGSVRWL"
     gene            477..1265
                     /locus_tag="PLB25_13635"
     CDS             477..1265
                     /locus_tag="PLB25_13635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOE42654.1"
                     /translation="MLLAAGRGERMRPLTDTCPKPLLRVRGKPLLQWHLEALLLGGFT
                     RAVINTAWLGPQIEQEFHGMFALQPLLDKRKQLLSQELLHLHYSREGEDFGAALETAG
                     GICRALPLLCPHLADGGSDVFWVVAADVFAPDFEFSRAAVEGFRASGKLAHLWLVPNP
                     EHKPGGDFALDADTGLALNLPDDDPRPRYTYSTIGLYRHALFAPPWCDIAAGNPAGMK
                     APLGPLLRLAMDKGQVSAELYTSAWTDVGTPERLAALNDMTTNA"
     gene            1433..3199
                     /locus_tag="PLB25_13640"
     CDS             1433..3199
                     /locus_tag="PLB25_13640"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOE42655.1"
                     /translation="MKTIARLDCLTSLRFFAAGMIVFHHSISLGLFGLGSTPGESSIF
                     GQGVSFFFVLSGFILAYVYQRLETWPKIFSFYRARVARVWPALVFSFFLTYWLLSLEW
                     DTPIALANLFMVNAWIPVPEYFFSYNIPSWSISTEFFFYLLFPVLIYKWEKYGRARLL
                     FSMLIVLIFCYISNTLQHSKDNLISAVALQAGLIYVNPLTRVLEFILGIFVCSIWRNL
                     RDRVQWRFAQATLYELLAVVVCALSVYYVPKFAWSMMLAFNESAITYWLIFGGSMLSF
                     SWLIYVMAIGKGWISRILSSSAPVLMGEISFSLYLVHLTLLRYYHENFSAFPVLPHGI
                     SMLIFWAVLLLVSYLMWALIEMPCRRLILGRDQAKVHGTNVLKETWHHHLNWNRKTVL
                     ASAMLISLLLSINLSMDQSAAVDLKRKNIPVDVDTLVYYAGTGNSKVINWLLQAGIDV
                     NARNSKGTIALTEAAWAGNDEVVSMLLTAGAKIEAKNANGLTALKAAVSQQQGVVALL
                     LLDRGASPHFVDADGTTLLMDAAWQGNLALVKKLLERGVDPNQRRPTDGMTALLLAKG
                     NRHTDVAQYLMSAGASGSDHPQ"
     gene            3257..3469
                     /locus_tag="PLB25_13645"
     CDS             3257..3469
                     /locus_tag="PLB25_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42656.1"
                     /translation="MHWPLRGIGAAAILMHGISVGAGPLWVPARYRCHRDAVELLPSG
                     RGAAFIEKWKSWQGSSDSSASGAGNA"
     gene            3498..3725
                     /locus_tag="PLB25_13650"
     CDS             3498..3725
                     /locus_tag="PLB25_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42657.1"
                     /translation="MSVYTVALAGEFDRGPVLREPQACAGACVMQCEGAAFSDSGKVG
                     AMPASNASMLASVFFPGQAAGELPKSSLQHR"
     gene            4039..4518
                     /locus_tag="PLB25_13655"
     CDS             4039..4518
                     /locus_tag="PLB25_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOE42658.1"
                     /translation="MSHKKSAGFTLIELVMVIVILGVLAAIALPRFVEMKADAEKSTV
                     EALVGSLATARTLWLTKAATCGSDYVAPGRMALATVVGLTDTRPAFCESPGYVQGHTF
                     DADQIRNGMMQNPAADLFTDNLDNGNQMQFVTKTGRTVTITHNPTTGGISYTAAPAY"
     gene            4554..>5121
                     /locus_tag="PLB25_13660"
     CDS             4554..>5121
                     /locus_tag="PLB25_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42659.1"
                     /translation="MVAGLAALALLFTGEIGLLTQYLLGKFAYSTSNAASLTLLAYLV
                     LAQWAGLWRSSSLSGPVALALMGAALVLGHGLNFWLTLRYLHEFELPLGVHLYHWQGA
                     GNSFTGLLHSHLGKTALAAGAQLLGWSSPAYDTGRVFLAWVPGFAPWALLTAFVVASL
                     GALAAIPTVWQRYAGHPAAMLLYVLSSLS"
BASE COUNT          961 a         1371 c         1500 g         1289 t
ORIGIN      
        1 caacaccggg ctgccgtcct cggtgggcgt ggtgcaggcg gtgttcaacc ccgacaatct
       61 tgattttgtg attgcctaca gcctggttgg cggggtcttt gccgcgctgg tttttgccat
      121 tgcggtggtg tcgatcccga tgattctgga ccgcgatacc gatgccatca gcgccgccat
      181 caccagcttg gaggtggtcg tcaacaacac tgccgtcatg ctgctgtggg gcgcagtgat
      241 tgtgctgctg gtggcggcat cgctgctgct gccctgggcg ctcggcctgc tggcggttgg
      301 gccgctgctg gggcatgcca gttggcatgc ctaccggggt tcggtgcgct ggctttgaat
      361 cgcggccagg gcgctggcac tgggctatgc tctgggcctc atctgatttt gtgaggccac
      421 ctttgtcaac gccaaactcc accttagcgc cgcgcaccag ccgtttggcg acggccatgc
      481 tgctggccgc tggccgcggc gagcgcatgc gcccgctgac cgacacctgc cccaaaccgt
      541 tgctgcgggt gcggggcaag cctctgctgc agtggcatct ggaagcgctg ctgctcggcg
      601 gctttacccg tgcagtcatc aacacggctt ggctagggcc gcaaattgag caggagtttc
      661 acggcatgtt tgccctccag cccttattgg ataagcgcaa gcagctatta agtcaggagc
      721 ttcttcatct ccactattcg cgcgaaggag aagactttgg tgccgctctg gagaccgctg
      781 gcggcatttg ccgggcactg ccgctgcttt gtccacacct tgcagacggt ggcagcgatg
      841 tgttttgggt cgtcgcggct gacgtttttg cgccagactt tgagttcagc cgggctgctg
      901 ttgaaggctt tcgcgccagc ggcaaactgg cccacctgtg gctggtgccc aaccccgagc
      961 acaaaccggg cggtgatttc gctctggatg cagacaccgg cctggccctg aacctgcccg
     1021 atgacgaccc gcgaccacgt tacacctatt ccaccatcgg cctgtaccgc catgcgctgt
     1081 ttgcgccacc ctggtgcgac atcgctgctg gcaacccggc tggcatgaag gccccgctgg
     1141 gcccgctgct gcgcctggcc atggataagg gccaggtctc tgcagagctg tacaccagcg
     1201 cctggaccga tgtcggcaca cccgagcgac tggcagcgct caatgacatg acgaccaatg
     1261 cctgaagttc gaggaacatg tgacaacgtg gtggtcaagc tcatattgct tcaatcacat
     1321 ttgaatgacg acctgctcaa ccaccagacg cagaccgtgc aaggccgcga tctgaacggt
     1381 tcaagaaaag ctgcttgatg cctgcatgcg ggcatggctg tggaataaac cattgaaaac
     1441 cattgcgaga ctcgattgcc tgacatcgct gcgttttttc gccgctggca tgatcgtttt
     1501 tcaccactca atatcactcg gattatttgg gctgggctcc acaccgggtg aatcttcgat
     1561 atttgggcaa ggggtttctt tcttctttgt tctttcaggt tttattctgg cctatgtgta
     1621 tcaacgcctg gaaacctggc cgaagatttt cagcttttac cgtgccagag tcgccagggt
     1681 ctggccggcg cttgttttca gttttttcct gacatattgg ctgttgtcgc tggagtggga
     1741 tacaccaata gcgctggcaa atttgttcat ggtgaatgcg tggattcccg ttcctgagta
     1801 ttttttctcc tacaacatcc catcgtggag tatttctacg gagtttttct tctacttgct
     1861 atttcctgtg ttgatctaca aatgggaaaa atatggaagg gcaaggttat tattttcaat
     1921 gctgattgtt ttgatatttt gttacatctc aaacacgctg cagcattcaa aggacaactt
     1981 gatttctgcc gtcgccctgc aggcaggatt aatctacgtg aatcctttga ccagggttct
     2041 tgaatttatt ctgggcatct ttgtatgttc catctggcgc aatctgcggg atcgggttca
     2101 gtggcgtttc gctcaggcaa cgctgtacga gctattggct gtcgtggttt gtgccttgtc
     2161 ggtctattat gttcccaagt ttgcctggtc catgatgctg gcgttcaatg agtcggccat
     2221 tacatactgg ttgatatttg gcggctcgat gctttcattt agctggttga tctatgtcat
     2281 ggcgattgga aagggctgga tcagtcgcat tttgtccagt tctgctccag ttttaatggg
     2341 cgagatcagc ttttctctgt atctggttca cttgacctta ttgcgatatt atcacgagaa
     2401 tttttctgct tttccagtcc tgcctcatgg catttcgatg ctgatttttt gggcagtttt
     2461 attattggtg tcttatttga tgtgggcact tatcgagatg ccttgcagaa gactgatatt
     2521 aggccgtgat caagccaagg ttcacggaac caatgtattg aaagaaacat ggcatcacca
     2581 tctgaattgg aatcgaaaaa ctgtgctggc ctcagccatg ttgatttcct tgttattgtc
     2641 catcaacctc tccatggatc aatcggctgc cgtggatctc aaaagaaaga atattcctgt
     2701 tgatgttgat acgcttgttt attatgcggg cactggtaac tcaaaggtaa tcaactggtt
     2761 gctgcaggcg ggtatcgatg tcaacgccag aaacagcaag ggcaccattg ccctgactga
     2821 ggcggcctgg gctggcaatg atgaagttgt ttcaatgcta ttgactgccg gcgcgaagat
     2881 agaggcaaaa aacgcaaatg gcctgaccgc actgaaagcc gccgtcagcc agcaacaggg
     2941 tgtggttgcc ctgctgctgc tcgaccgagg tgccagccct cattttgttg atgctgacgg
     3001 cacgaccctg ctgatggatg cagcctggca aggcaatctg gcgctggtca aaaaattgct
     3061 ggagcgtggt gtcgatccaa accagcggcg acccacggat ggaatgaccg cattgctgct
     3121 tgcaaaaggc aatcgccata ccgatgtggc acagtacctg atgtcagcgg gtgcatcagg
     3181 gtctgaccat ccgcagtgat ggggcagggc gctggcagac ctttcccgtc ggccacgagg
     3241 gcaaaattgc gcaggtgtgc attggcccct gcggggcatt ggtgcggcgg ccattctgat
     3301 gcacggtatc agcgtgggtg ccgggccatt gtgggtgcca gcgcgctacc ggtgccatcg
     3361 cgatgcggtt gagttgctgc catccgggcg gggtgcggct ttcattgaga agtggaaatc
     3421 atggcaagga tcaagcgatt cgtcggcaag cggtgcaggc aatgcctgac taccggttgc
     3481 gttcgacgct tgttgttttg agcgtatata ccgtggcgct ggccggggaa tttgacagag
     3541 gcccggtctt gcgcgagccg caggcttgtg ccggtgcttg tgtgatgcag tgcgagggcg
     3601 cagcctttag cgactcgggc aaggtgggtg cgatgcctgc ttcaaatgcc agcatgttgg
     3661 catcagtatt ttttcccggc caggcggccg gtgagttacc caagagcagt cttcaacaca
     3721 ggtaattcca tctctaaatc attcttgtct aggcaccctg ccgcagatat tgcaaacgca
     3781 aggcaaggcg atataagcac gacatggatg atttggaaac ggaataaacc aacaagaacg
     3841 aacagatttg ccctgttcat cggtaggctc cggctgttcc gacggccagc cttgaggtaa
     3901 agtgtgcggg ttggatgcct cgaatggaac atggtttttt gcattccttt tgacgctgtc
     3961 aaataacccg gccaaagtca gactggtatc ggtcagaatt ttttgaactg tcgaatggca
     4021 gtgaaccctg gagttcccat gagccacaag aaaagcgccg gatttacact gattgaactg
     4081 gtcatggtga ttgtcattct gggtgtgctg gctgccatcg ccttgccgcg ttttgtcgaa
     4141 atgaaagccg atgcagaaaa gtcaacggtc gaggcgctgg tgggttcgct ggccacggcc
     4201 aggacgcttt ggctgaccaa ggcggcaacc tgtggctctg attacgtggc accggggcgc
     4261 atggcgctgg ccacggtggt tggcctgacc gacaccaggc cggcattttg tgagtcacct
     4321 ggctatgtgc aggggcacac cttcgatgcc gatcagattc gcaacggcat gatgcaaaac
     4381 ccggcagccg acctgttcac cgacaacctt gacaacggca accagatgca gtttgtcacc
     4441 aaaactggcc gtaccgtgac gattacccac aacccgacca ctggcggcat cagctacacc
     4501 gctgccccgg cctattagcg ccctggccgg tgcgggcggc gttgctgatt tcaatggtcg
     4561 ccggattggc ggctttggcc ctgctgttta ccggcgaaat tggtctttta acccagtatc
     4621 tcctgggcaa atttgcctac agcacctcaa atgcggccag cctgacgctg ctggcttacc
     4681 tggtattggc ccaatgggcg ggcctgtggc gcagcagcag cctgagcggc ccggtggcgc
     4741 tggcgctgat gggggctgcc ctggtgctcg ggcatggcct gaatttctgg ctgacgctgc
     4801 gctacctgca cgagtttgaa ctgccgctgg gcgtgcatct ctaccattgg cagggggctg
     4861 gcaactcttt caccggcctg ctgcacagcc atcttggcaa aactgcgttg gccgccggtg
     4921 cgcagttgct gggctggtca agcccggctt atgacactgg tcgggtcttt ctggcctggg
     4981 tgccgggctt tgcaccctgg gcgctgttga ccgcctttgt cgtcgccagc ctgggcgctc
     5041 tggcggctat cccgaccgtc tggcagcgtt atgccggcca tccggcggcc atgctgcttt
     5101 atgtgctgag ttccttgtcc t
//
LOCUS       DAOSLL010000322         5104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14596_length_5104_cov_4.368390, whole genome shotgun sequence.
ACCESSION   DAOSLL010000322 DAOSLL010000000
VERSION     DAOSLL010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14596_length_5104_cov_4.368390"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1298
                     /locus_tag="PLB25_13665"
     CDS             <1..1298
                     /locus_tag="PLB25_13665"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HOE42660.1"
                     /translation="SALLVKEAFKTWGDAVGEVREAIDFLRYYAQQAERIMQPQIMPG
                     FSGNTLLGVTGESNELTLTARGPWVCISPWNFPLAIFLGQVAAALATGNTVLAKPAEQ
                     TPGVALEAVKLLHAAGVPEGALQLLHGPGETVGAALVAAAGVAGVVFTGSTAVAKLIN
                     RTLAAKDGPIMPLIAETGGINAMLVDSTALPEQVCDAVMQSAFRSAGQRCSALRLLCV
                     HEQIADHVIEMICGAARELVTGDPAELATDIGPVIDAQAFAGIQQHLQRLNIEAKHLP
                     AHTGKALPATTKIANLIAPQAFEVSSINDLKAEIFGPVLHIVRWTGDPLAVIAQINAL
                     GSSAAPAGATPGYGLTLGIQTRIDSRAQQLARAAHIGNVYINRNQIGAVVGVQPFGGE
                     GLSGTGPKAGGPHYLYRFCAEQTITVNTTAAGGNVQLMA"
     gene            complement(1672..3165)
                     /locus_tag="PLB25_13670"
     CDS             complement(1672..3165)
                     /locus_tag="PLB25_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42661.1"
                     /translation="MNPSPTCEVPTMLQTDFKALREAIAAIPADVSNDAVGQYAPRLQ
                     DIFAPETHAAALAPGTPIVVGPRGAGKSFWSGVLGQDETRAAAAKAYPQLGLDRVQVG
                     FGFTGVGASEGVTPEMLNSFVGADATSQDAKGFWWATILRAASKGAGHAPMSFKEAVR
                     IGLDWEERNLVLGKLEVHFQDQNSTFLLVYDALDTVAMTWPRRRLLTEALLEVVWAMR
                     AYRHIKVKLFLRPDQIDDDALRFVELPKLRTGAVRLQWSGVDLYGLLYARLSLSPLDP
                     AKVAFNQLLEGLNISAADQNSILARQWTLAHDAKNQSRVMEVLAGAYMGDGPSGYKKG
                     KTYEWPVAHLADAFAEVTPRSFLGLMIAAAKYGAVPNDRVITPEGIRHGLRAASKTRV
                     DQLHQEFLWIKGVLAPLAGLLLPQSEDEVFNAWRKTKTLAQLTADAKASKYLPPFEIA
                     KSNSERDLFLALQKIGVMFSRKDGRLDMPDLFRVAARLLKKGGTAPV"
     gene            complement(3119..4498)
                     /locus_tag="PLB25_13675"
     CDS             complement(3119..4498)
                     /locus_tag="PLB25_13675"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE42662.1"
                     /translation="MTIRFDDSLPALVSVIRTGFGSAALAQGVAVRDVTGRLAFVLPR
                     EFDAELVERIGNLLIDELGPYARTDRVLVNATDFGAEALLKTESALSVKVNDSAIRLI
                     DRRLVGADWLRTPAAKAPPPPRFVFASLKGGVGRSTALAITAAHLASAGKRVLAIDLD
                     MEAPGLGSLLLDDGTLPEFGLIDALVENGLAPLDEAFLADLVGPSDLADRRGRIDVIP
                     AFGRRSIQNPGDVLSKIARAYTEDIRPDGSMASILDQVAELFDRFADPARYDAILVDA
                     RAGLHETTATAILGLGAEVFLFGLDEAQTFQGYAALLGHLGRFAKSSTAEVPEWLGRL
                     SMVQGKAPADENAREAFADKCKQMFIASGLESVGLQPPTEPLLGAGSFSDVPWDDDQS
                     DEDVLPTEQDVFRAPVAILNNEQFMGFDPRQKRSLLSQDVYRFSYGMFLTRVDESFPD
                     LRSADHAAN"
     gene            complement(4507..4902)
                     /locus_tag="PLB25_13680"
     CDS             complement(4507..4902)
                     /locus_tag="PLB25_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42663.1"
                     /translation="MTFPPNMAASARRHLHAAEALDAGHRRDVAGYLYGIAAECAIKA
                     MMLDAGLRPTGDQRNDPFYLHFPQLRTALCDNMAGRRATPLMNFIGSSSFLSHWATDM
                     RYCKGDEILGKWVDLWSSQARQAVSSMGT"
BASE COUNT         1105 a         1525 c         1455 g         1019 t
ORIGIN      
        1 ttagcgccct gctggtcaaa gaggccttca agacctgggg cgatgcggtc ggcgaggtgc
       61 gcgaggccat cgactttctg cgctactacg cgcagcaagc cgaacgcatc atgcagccgc
      121 aaatcatgcc gggcttttcc ggcaacaccc tgctgggcgt caccggcgaa agcaacgagc
      181 tgaccctgac tgcgcgtggc ccctgggtct gcatcagtcc gtggaacttt ccgctggcga
      241 tctttttggg tcaggtcgcc gccgcgctgg ccactggcaa cacggtgctg gccaaacccg
      301 ccgagcagac acctggcgtt gcgctggaag ccgtcaagct gctgcatgcg gctggcgtac
      361 ccgagggcgc gctgcaactg ctgcacgggc caggtgaaac agtcggtgcg gcactggtgg
      421 cagcggcagg tgtggcgggt gtggtgttca ccggttcaac tgcggtggcc aaactcatca
      481 accgcacgct ggccgccaaa gacggcccga tcatgccgct gattgctgag accggcggca
      541 tcaacgccat gctggtcgat agcaccgcgc tgcccgagca ggtgtgtgat gcggtgatgc
      601 agtcggcctt cagaagcgcc ggccagcgct gctcggcgct gcgcctgctg tgcgtgcacg
      661 aacagattgc cgatcatgtg atcgagatga tttgcggcgc ggcgcgcgaa ctggtgacgg
      721 gcgacccggc cgagctggcc accgacatcg ggccggtgat tgatgcccaa gcctttgccg
      781 gcatccagca gcacctgcag cgcttgaata ttgaggcaaa acaccttcca gcccatacag
      841 gcaaagcgct accagctacc acaaaaatag caaacctgat cgcaccgcaa gcctttgaag
      901 tcagcagcat caacgacctg aaagctgaaa tttttggccc ggtgctgcac atcgtgcgct
      961 ggacgggcga cccgctggcg gtgattgcgc aaatcaacgc gctaggaagt tcggctgcgc
     1021 ccgccggtgc aacgcctggc tacggcctca ccctgggcat ccagacccgc attgactcca
     1081 gagcccagca actggcgcgc gccgcgcaca tcggcaacgt ctacatcaac cgcaaccaga
     1141 ttggcgcggt ggtgggcgtg cagccgtttg gtggcgaagg cctgagcggc accgggccga
     1201 aagccggcgg gccgcattac ctgtatcgct tttgcgccga gcagacgatt accgtcaaca
     1261 ccacggcggc gggggggaat gtgcagttga tggcgtgagc gtggggggtg tgacggcaag
     1321 aacgcccgcg agcctgcgct cagaatcggg ccatgggcgt ttcggggcgc ttcgcaggac
     1381 acgcgctgta gcagatttgg ggtcggatcc caattcggcc cgacacgatt gtgttcgtca
     1441 ccaacgcgcc ccgaattggg ctctgacccc aattctgcgg ggtgtagcga ctgcgctctt
     1501 ttgtgctttg gaggtggcat ggctaccagg gaaaaatatc agagttcgag cggccaaacc
     1561 agaaccagtt tgcaggggaa tacagcgctt tcatcgttcg ccctggccgc aggcgtattg
     1621 aatacgacat ggcgaaggcg atgcaaactt gaggttgctg cacgctccca tctacacggg
     1681 tgccgtgccg ccctttttca gcagccgcgc ggcgactcta aataggtcag gcatatccag
     1741 ccgcccgtct ttgcgactga acatcacgcc aattttttgc aatgccaaaa acagatcgcg
     1801 ttcgctgttt gatttggcga tctcaaacgg gggcaggtat ttgctggcct tggcatctgc
     1861 cgtcagttgc gccagggttt tggtcttgcg ccaggcattg aaaacttcgt cttcgctttg
     1921 cggcagcagc aagcctgcaa gcggggcgag tacccccttg atccagagaa attcttgatg
     1981 caattggtca acacgggttt ttgatgcagc ccgcaatcca tgtcgaatgc cttctggcgt
     2041 tatcacacga tcattgggca ctgcgccgta cttggctgcc gcaatcatca gccccaggaa
     2101 acttcggggc gtcacctcgg caaaagcatc ggcaaggtgg gccaccggcc attcgtaagt
     2161 cttgcctttt ttgtagccac tggggccgtc acccatataa gcaccggcta agacttccat
     2221 cacccgcgat tggttctttg cgtcatgcgc cagcgtccac tgccgggcaa gaatcgagtt
     2281 ctgatccgcg gctgaaatgt tgaggccttc taacagttga ttgaacgcca ctttggcagg
     2341 atcaagcggc gagagactga gccgggcgta aagcagtcca taaagatcaa caccggacca
     2401 ctgcaagcgc acagcgccag tacgcagctt gggcagttcc acgaagcgca gcgcatcgtc
     2461 gtcaatttga tcggggcgca ggaacagctt gactttgata tggcgatagg cacgcatggc
     2521 ccagacgact tccagcaaag cttcagtcag gagtcggcgc ctcggccagg tcattgccac
     2581 cgtgtcgagc gcatcgtaaa ccagcagaaa tgttgagttc tggtcttgaa aatgaacctc
     2641 cagcttgccg aggaccaggt ttcgctcttc ccaatccaaa ccaatgcgta ccgcttcctt
     2701 gaaactcatg ggtgcatgac cggcaccttt gcttgccgcg cgcaatatgg tggcccacca
     2761 aaaacctttg gcatcctgcg atgttgcatc agctccgaca aaagaattca gcatctctgg
     2821 tgtcaccccc tcgcttgcac ccacaccggt gaaaccaaaa ccgacctgca cccgatccag
     2881 accaagctgt ggataggctt tggccgctgc cgcacgcgtt tcgtcctgtc ccaaaacgcc
     2941 cgaccaaaag cttttgccgg caccacgcgg ccccaccaca atcggtgtgc ctggcgccag
     3001 ggctgcggca tgggtttctg gtgcaaaaat atcttgtaac ctgggtgcgt attgacctac
     3061 tgcgtcattc gacacatcgg caggaatggc ggctatcgcc tcacgcaatg ccttgaaatc
     3121 agtttgcagc atggtcggca cttcgcaagt cggggaagga ttcatcgaca cgggtcaaaa
     3181 acatgccgta agaaaagcgg taaacatctt gggaaagcag acttctcttt tgtcgcggat
     3241 caaaccccat gaactgctca ttgttcaaaa tcgccactgg tgcccgaaat acgtcctgct
     3301 ctgttggcag aacgtcctca tcgctctggt cgtcgtccca aggcacatca ctgaatgaac
     3361 cggcaccaag tagtggctct gtgggcggct gcaaaccaac ggattccagt ccactggcaa
     3421 taaacatctg tttgcacttg tccgcaaatg cctcccttgc attttcatca gcgggtgcct
     3481 tgccctgcac catggacagt cggcccagcc attcgggcac ttcggcagtg gacgacttgg
     3541 cgaagcggcc caaatgcccc agcaatgccg cgtagccttg aaaagtctgt gcttcatcaa
     3601 ggccaaatag aaagacctcg gcccccaagc caagaatggc cgttgccgta gtttcatgca
     3661 aaccggcacg cgcatcgacc aggatcgcgt cgtagcgcgc tgggtcggca aagcggtcaa
     3721 acagttcagc gacctgatca agaatggaag ccatgctgcc gtctggccgg atgtcttcgg
     3781 tgtaggcgcg ggcaattttc gacagcacgt cgcctggatt ttggatggag cggcgtccaa
     3841 acgctggaat gacatcgatt cggccacgtc gatctgccag gtcagaaggg ccaaccaggt
     3901 cggccagaaa ggcttcatcc aagggtgcca gaccgttttc gaccagggca tcaatcagcc
     3961 caaattcggg caaggtgcca tcatcgagca gcagtgaccc gagtcctggc gcttccatgt
     4021 caaggtcaat cgccaacacg cgctttccgg cagaagccaa atgtgcggct gtgatagcca
     4081 acgcggttga ccggcctacg cctcccttga ggctggcaaa aacaaaacga ggaggcggcg
     4141 gcgcctttgc agcaggagtg cgaagccagt ctgcaccaac cagtcggcga tcaatcagcc
     4201 tgatggcgct gtcgttgact ttgacggata aggcggactc tgtcttcagc aaagcctcgg
     4261 ccccaaagtc ggtcgcgttg accaatacac gatcagtgcg tgcgtagggg ccaagttcgt
     4321 caattaaaag attgccgatg cgctcgacca gttcagcgtc aaactcacgt ggcaggacaa
     4381 atgccaggcg accggtaacg tctcgcacag cgacaccctg cgcgagtgcc gcactgccaa
     4441 acccggttct gataaccgat accagtgccg gaaggctatc gtcaaatcga atggtcatat
     4501 atgccctcaa gtccccatgg atgaaacagc ttgacgcgct tggcttgacc aaagatcaac
     4561 ccatttgccc aaaatttcat ctcctttgca gtaccgcata tcagtcgccc agtggcttaa
     4621 aaaagaactg ctgcctataa agttcattag aggtgttgca cgtctgccag ccatgttgtc
     4681 acacagcgcg gtccgcaact gcggaaagtg caaatagaac ggatcattgc gctgatcacc
     4741 ggtggggcgt aagcctgcat caagcatcat cgccttgatc gcacattcag cggcaatgcc
     4801 gtagagatag cctgccacat cacgacgatg cccggcatcc aatgcttctg cagcatgcag
     4861 atgtcttcgg gcagatgcgg ccatgtttgg tggaaaagtc atcccgatcc tctgagtttt
     4921 gaacgatggt aacgaaactt gcggcactaa cggttgattt tggactgcat catcttccca
     4981 cttgagtatc ggccagcgct gctggcggtg cccgacgcga tctacccgca gtttgccacc
     5041 cacaacgccg ccaccgtggc cgccattttg cagatggcgg cacgcaccgg cgcgccgttt
     5101 gagc
//
LOCUS       DAOSLL010000323         5100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14613_length_5100_cov_4.109415, whole genome shotgun sequence.
ACCESSION   DAOSLL010000323 DAOSLL010000000
VERSION     DAOSLL010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14613_length_5100_cov_4.109415"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(419..937)
                     /locus_tag="PLB25_13685"
     CDS             complement(419..937)
                     /locus_tag="PLB25_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42664.1"
                     /translation="MSMTHYMELLAQNQPWNLILFMAIPVILAETVAITELFILFSRQ
                     FHGALRQVNRSASIAGGLYFCGVFVYLMATAVIPLTSSGGWRGAADVIAVGFYLSGIV
                     PLLGLALLDLGVIGKARTDEGKLKLHATFVAIFLVVAHIAMIFGMLDPTLLMSQAPDA
                     MHNMGSMPGMQH"
     gene            complement(1015..1371)
                     /locus_tag="PLB25_13690"
     CDS             complement(1015..1371)
                     /locus_tag="PLB25_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42665.1"
                     /translation="MHLLVRFLFVLCALSGLAATPAWACESMTRTVSAASSNHPHDAF
                     AATAAPVAAPANALPDMCAAAPNCDRCASCVSCVGLVPVIVDTWFVPGTSNLPQWTPQ
                     PLARLGAAPELKPPRA"
     gene            2002..2559
                     /locus_tag="PLB25_13695"
     CDS             2002..2559
                     /locus_tag="PLB25_13695"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE42666.1"
                     /translation="MVSIVIVPGWQDSPPGHWQSLWAQQLPDAIRVQQDDWAAPSRSA
                     WVASISRSVLACSGPVIIAAHSLGCIATTHLPPEVVARIQGALLVAPADPERRSVLVD
                     FAPVPYGRLPYHSIIVASSNDPYCPVRLAGAYARAWGSEFVRLHNLGHINLESGFGPW
                     PLGLALLQTLISEADCRQEAALSFA"
     gene            2606..3607
                     /locus_tag="PLB25_13700"
     CDS             2606..3607
                     /locus_tag="PLB25_13700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE42667.1"
                     /translation="MNSKQKTMLALASIALAAPQIVASQTTLLNASYDVSREFYKDYN
                     TAFVAHYKKTTGKDVKIDQAHGGSSAQARAVNDGLDADVVTMNTTTDVEFLANAGVVA
                     KDWTRKFPNNAAPTTSTMLFLTRNGNPKAIRDWNDLVKPDIKVIVVNPKTGGNGRMAY
                     LAAWGYVRKTGGSDAQAAEFVAKLFKNVPVLAKGGRDATTIFLQRNIGDVLITFESEV
                     VSVDREFGAGKVDAIHPSVSIIAENPVAVVERTVAKKGTGELARAYLAYLYSDEAQEI
                     AAKHGIRPSNKQVLAKYASTFKPLQLFAVSDYFGSLSEAQKVHFNDGGQFDKIYTVK"
     gene            3670..4560
                     /gene="cysT"
                     /locus_tag="PLB25_13705"
     CDS             3670..4560
                     /gene="cysT"
                     /locus_tag="PLB25_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HOE42668.1"
                     /translation="MTSAILSTTPATVLSLNTASRRPARRVLPGFNLTLGYTLFYLGL
                     IVLIPLSALLFKTFTLSWDQFWLAVTSPRVLASYRLTFGASFLAALVNVVAGLLLAWV
                     LVRYQFPGKKIIDALVDLPFALPTAVAGISLTALLAGNGWIGQYLEPHGIQLAFNRNG
                     VVIALIFIGLPFVVRTVQPVLEDAEKELEEAATCLGASRFQTFRYVIFPTIAPALLTG
                     FAMAFARAIGEYGSVIFIAGNMPMVSEITPLIIIGKLEQYDYAGATAVATVMLAISFV
                     LLLVINALQSWQRSRSGGKS"
     gene            4557..>5100
                     /locus_tag="PLB25_13710"
     CDS             4557..>5100
                     /locus_tag="PLB25_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018904514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HOE42669.1"
                     /translation="MSAPTLGRAEAGTSEPAWVRRILIGLALGFVFLFLLLPLAAVFT
                     EALRKGLPAALEALHEPDAWSAIRLTLITAAVAVPLNLVFGVAAAWAVAKFEFKGKAF
                     LTTLIDLPFSVSPVVAGLIYVLMFGAQGWIGPWLADHNIKIVFAVPGIILATIFVTFP
                     FIARELIPLMQAQGTDEEQAA"
BASE COUNT         1062 a         1523 c         1427 g         1088 t
ORIGIN      
        1 agtcgcgccc tcgcgtcgaa tgactttgat tgtttgaatt ctttgcatga tatgcaaaga
       61 cttgacttgg tagagattga gttcctcagt tgaccgctcc ttttcagctc caagtcctta
      121 tcagcatccc aaccccgcac gcgggccagg cgttctcaaa gcaaattgac gttgaggccg
      181 gctgcgcagt tggcgcaagc cacccagcct ttatctgcca aatatggctc tatcccatac
      241 agcttatgtg cttctagcta ctattttgat tgtcaacaac atatctgtac ctagtaagca
      301 cttgcttttt gagcttcgat ggggttcacc cattgggtca cggcgctacg cgcccgattg
      361 aaccgctggc aacatggaac gccggatcga caggcgctcc accacccttg atttcagctc
      421 aatgctgcat gccgggcatc gaccccatgt tgtgcattgc atcgggtgcc tgactcatca
      481 gcaaggtcgg gtccagcatg ccaaaaatca tcgcaatgtg agccaccacc agaaagatgg
      541 cgacaaatgt ggcgtgtaat ttgagcttgc cctcgtcagt gcgggccttg ccgatcaccc
      601 cgaggtcaag caaagccagg cccagcaacg gcacgatgcc cgagaggtaa aaccccaccg
      661 caatcacatc agcggcaccg cgccacccgc ccgagctggt cagcggaatc acggcagttg
      721 ccatcaggta aacaaacacg ccgcaaaagt acaacccacc ggcaatgctg gcactgcggt
      781 tgacctgacg cagcgcgccg tgaaattggc gggaaaacag aatgaacaac tcggtaatgg
      841 ccacagtttc agccaggatg accgggattg ccatgaacaa aatcagattc cagggctggt
      901 tttgcgccag caactccatg tagtgcgtca tagacatgat gaattctcca aagataaaca
      961 agtgaaacga ccgcactgcc agcaggcaag gcggtcactg caaagacagg agaatcacgc
     1021 gcgcggcggt ttgagttcgg gcgcagcacc caggcgggcc aatggctggg gtgtccattg
     1081 cggcaaattg ctggtgccgg gaacgaacca ggtgtcaaca atgaccggaa ccaggccgac
     1141 gcaagaaaca cagcttgcgc aacggtcgca attgggagca gcagcgcaca tgtcgggcaa
     1201 agcatttgct ggtgcggcaa ccggcgctgc ggtagcagca aacgcatcat gcgggtggtt
     1261 tgaagaagcc gctgacaccg tccgggtcat cgattcgcaa gcccaggcag gtgtcgcggc
     1321 cagccccgac agcgcgcaaa gcacaaagag gaatcgaaca agcaggtgca tgaaggtggt
     1381 caatacgtca accgggtcat tctacaggtg ggtccccagg tgcggaacgc acagtgcttt
     1441 tgctaaccag atctccaatg gatgaaatgg aatgtcattc gttggaggct cagcaataac
     1501 acggggcaag gccactgcca ttggctcgac atccgatttc agacctcctc atggccttca
     1561 tcttcttcat tccaggctgg ccaaaatcgg cccgtcagcc actggcaaag ccgttgggcc
     1621 aagccgttgc caggcgtgcg gcacgggtag ggtgattgac cagaaggcta tcgggctcat
     1681 ttcatcgatt gcgggaatcg accccagcgg agggtgccgg acttgtctgc ccatgcaagc
     1741 cctggcccaa aagaccgctg cataaatggt ccgcagcagt cggtttgcag ccaatttcgc
     1801 caccaatact cccgatgtcc gacagtctgc tggcaccccg cgcagcgcct cattcgccaa
     1861 gacaagggcc ggccgcctgc cccaatgttt ccccatataa cgttttcaaa taagcaaata
     1921 gaaattaagt cgtttgtgtt tgaacgactg aaacccacaa tgcgggctct tcatgttctg
     1981 cattcgaccc aatacccgat catggtttcc atcgttatcg ttccaggctg gcaagactcg
     2041 cctcccggcc actggcaaag cctgtgggcg caacagttgc ccgacgccat ccgggtgcaa
     2101 caggacgact gggctgcacc ttctcgcagc gcctgggtgg cctcgatttc ccggtcggtg
     2161 ctggcttgct ccggcccggt gatcatcgct gcccatagcc tgggttgcat tgccaccacc
     2221 catctgccac cagaggtggt cgcgcggatt cagggcgcac ttttggtggc accggctgac
     2281 ccggagcggc gcagcgtgtt ggttgacttt gccccggttc cctatgggcg cctgccatat
     2341 cacagcatca ttgtggccag cagcaatgac ccgtattgcc cggtgcgttt ggcaggcgct
     2401 tatgcccggg cctggggcag cgaatttgtc cgccttcaca accttgggca catcaatctg
     2461 gaatctggtt tcgggccctg gccactgggc ctggcgctgc ttcagacatt gatcagtgaa
     2521 gccgattgtc ggcaggaagc cgctttgtct ttcgcctgac atcagcagac aactttttct
     2581 atttcaatac ctttatcacg tctttatgaa ctccaaacaa aaaaccatgc tagcccttgc
     2641 cagtattgcg ctggcagctc ctcaaatagt agcatctcaa accactttgc tgaatgcgtc
     2701 ttacgatgtc tcgcgtgagt tttacaaaga ctacaacacg gcatttgtgg cgcactataa
     2761 aaagaccact ggcaaggatg tcaagatcga ccaggcccac ggtggctcca gcgcacaggc
     2821 acgtgccgtc aatgacggac tcgatgccga tgtggtcacc atgaacacca ccaccgatgt
     2881 tgaatttctg gccaacgctg gtgtcgtggc caaggattgg accaggaagt tccccaacaa
     2941 tgccgcgccg accacctcga ccatgctgtt tctgacccgc aacggcaacc ccaaggcaat
     3001 ccgcgattgg aacgacctgg tcaagcccga catcaaggtg atcgtggtca accccaagac
     3061 cggtggcaat ggccgcatgg cttatctggc ggcctggggc tatgtgcgca agactggcgg
     3121 cagcgacgcg caagcggccg aatttgtcgc caaactgttc aagaacgtgc cggtgctggc
     3181 caagggcggg cgcgatgcca ccaccatctt cttgcagcgc aacatcggcg acgtgctgat
     3241 caccttcgag tccgaggtgg tgtcggtaga tcgcgaattt ggtgctggca aagtcgacgc
     3301 cattcatccc tcggtcagca tcattgccga aaacccggtg gccgtggtgg agcgtaccgt
     3361 ggccaagaaa ggcactggtg aactggcccg ggcctacctg gcctacctgt actctgacga
     3421 ggcgcaagaa attgccgcca aacacggcat tcgccccagc aacaagcagg tgttggccaa
     3481 gtacgccagc accttcaaac cgctgcaact gtttgcggtg agcgactact ttggctcgct
     3541 gtctgaagcg caaaaggtgc acttcaacga cggtggtcag ttcgacaaaa tttacaccgt
     3601 caagtagaca ttggtccgcc cacccgatgg cgacccaggt tcagtacgac aactccagaa
     3661 gcgaatcaga tgacatctgc cattctttcc accacgcctg ccacagtgct gtcgctcaac
     3721 acggcatcgc ggcgaccggc ccggcgcgtc ttgccaggtt ttaacctgac cctgggttac
     3781 accctgttct accttggcct gattgtgctg atcccgctgt cagccctgct gttcaagacc
     3841 ttcacactga gttgggatca gttttggttg gctgtgacca gtccacgcgt attggcctcg
     3901 taccggctga cctttggcgc atcgttcctg gcggcgctgg tcaatgtggt tgccggtctg
     3961 ctgctggcct gggtgctggt gcgctaccag tttccgggca aaaaaatcat cgacgcgctg
     4021 gtggacctgc cctttgccct gccgactgcc gtagccggga tttcactgac agcattgctt
     4081 gctggcaatg gctggattgg ccaatacctg gagccgcacg gcatccagtt ggccttcaac
     4141 cgcaacggtg tcgtgatcgc gctgatcttc attggtttgc cgtttgtcgt gcgcaccgtg
     4201 caaccagtgc tcgaagatgc cgagaaagaa cttgaagagg ctgccacctg cctgggtgcc
     4261 tcgcgctttc agacctttcg ttatgtgatt ttcccgacga tcgcgccagc cctgctgacc
     4321 ggctttgcca tggcttttgc cagagccatt ggcgaatatg gctcggtgat cttcatcgca
     4381 ggcaacatgc cgatggtgtc cgagatcacg ccgctgatca tcatcggcaa actcgaacaa
     4441 tatgactatg ccggtgccac cgctgtggca acggtcatgc tggccatatc gtttgtgttg
     4501 ctgctggtga tcaacgcgct gcagagttgg cagcgtagcc gctccggggg caagtcatga
     4561 gcgccccgac cctgggtcgg gctgaagccg gcacctcgga gcccgcctgg gtgcgccgca
     4621 tcctcattgg cctggcgctg ggctttgtct ttttattcct gctgctgccg ctggccgcag
     4681 tgtttaccga agccttgcgc aaagggctac ctgctgcgct ggaggctctg cacgagcccg
     4741 atgcctggag cgccattcgc ctgaccctga tcaccgctgc cgtggcggtg ccgctcaatc
     4801 tggtgttcgg ggtggcggcg gcctgggcgg tggccaaatt cgaattcaag ggcaaggcat
     4861 tcctgaccac gctgattgac ctgccgtttt cggtctcgcc ggtggtggcg ggtctgatct
     4921 atgtgctgat gttcggtgcc cagggctgga ttggcccctg gctggcagat cacaacatca
     4981 aaatcgtgtt tgccgtgccg ggcatcattc tggccaccat ctttgtcacc ttccctttca
     5041 ttgcgcgcga gctgattccg ctgatgcagg cgcaaggcac cgacgaggag caggcggcca
//
LOCUS       DAOSLL010000324         5097 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14626_length_5097_cov_5.210432, whole genome shotgun sequence.
ACCESSION   DAOSLL010000324 DAOSLL010000000
VERSION     DAOSLL010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14626_length_5097_cov_5.210432"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="PLB25_13715"
     CDS             complement(<1..438)
                     /locus_tag="PLB25_13715"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HOE42670.1"
                     /translation="MDIALLVKAAIMGLVEGLTEFLPISSTGHLILAGALLGFDDEKG
                     KVFDIAIQTGAIFAVILVYWQKIRDTVVALPSDRQAQRFALNVLIGFLPAVVLGLLFG
                     KAIKAHLFTPVVVASTFILGGLIILWAEKRQKFDEDAPGSGVAN"
     gene            997..2061
                     /gene="rfbB"
                     /locus_tag="PLB25_13720"
     CDS             997..2061
                     /gene="rfbB"
                     /locus_tag="PLB25_13720"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HOE42671.1"
                     /translation="MILVTGGAGFIGANFCLDWLAQSDEPVVNLDKLTYAGNPETLQS
                     LQGDARHTLVHGDIGDSALVSQLLTTHQPRAIVNFAAESHVDRSIHGPGEFIHTNIVG
                     TYELLEAVRQYWSALAAPEKVAFRFLHVSTDEVYGSLAKDDPAFTESSHIEPNSPYSA
                     SKAASDHLVRAWHHTYGLPVLTTNCSNNYGPYHFPEKLIPLMIVNALAGKSLPVYGDG
                     QQIRDWLYVKDHCSAIRRVLQAGRPGETYNVGGWNEKANLEIVHTVCALLDELRPRAD
                     GKPYRAQITYVTDRPGHDRRYAIDASKLERELGWKPAETFETGIRKTVQWYLDNPDWV
                     AHVQSGAYRAWVQKQYADTP"
     gene            2084..2983
                     /gene="rfbD"
                     /locus_tag="PLB25_13725"
     CDS             2084..2983
                     /gene="rfbD"
                     /locus_tag="PLB25_13725"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HOE42672.1"
                     /translation="MKILLFGKNGQVGWELQRSLSPLGELVALDSDSTDLCGNFTDLA
                     GLAQTVRSVRPDVIVNAAAHTAVDKAESEPDLARTINALAPEVLAREAARLGAWLVHY
                     STDYVFDGSGDKPWLETDATGPLSVYGRNKLEGETLIALHNPRHLIFRTSWVYAARGG
                     NFAKTMLRLGQEREKLTVINDQIGAPTGADLLADVTAHALRQVLPGASPEHIAGLYHL
                     VAAGETNWFDYAKHVLAQALIAQPAIKIVAQQIDPVPTSAFPTPARRPHNSRLNTGKL
                     QRTFGLRLPPWQHGVNRMLAEIL"
     gene            3002..3874
                     /gene="rfbA"
                     /locus_tag="PLB25_13730"
     CDS             3002..3874
                     /gene="rfbA"
                     /locus_tag="PLB25_13730"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HOE42673.1"
                     /translation="MTTRKGIILAGGSGTRLYPVTQAVSKQLMPIYDKPMVYYPLSTL
                     MLAGIREVLIISTPQDTPRFADLLGDGSQWGMSISYAVQPSPDGLAQAFIIGRDFVAG
                     QPSALVLGDNIFYGHDLVKQLAHANARTVGASVFAYHVTDPDRYGVVAFDPQMRAVSI
                     EEKPTQPQSNYAVTGLYFYDQQVCDIAADIKPSRRGELEITDVNRRYLQQGQLNVEIM
                     GRGYAWLDTGTHDSLLEAASFIATLQKRQGLQVACPEEIAYSQKWIDAAQILKLTTPL
                     AKNGYGQYLLSLLK"
     gene            3953..4504
                     /gene="rfbC"
                     /locus_tag="PLB25_13735"
     CDS             3953..4504
                     /gene="rfbC"
                     /locus_tag="PLB25_13735"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HOE42674.1"
                     /translation="MPYTVVATSLPEVLILEPKVFGDARGFFYESFNARDFTQCTGLD
                     VQFVQDNHSKSAKGVLRGLHYQIQHPQGKLVRVTQGEVFDVAVDLRRSSPKFGRWEGV
                     VLSAENKRQLWVPPGFAHGFVVLSESAEFLYKTTDYWYPEFERSLLWSDPTIGVQWPL
                     GNPPLLAAKDRDAATLEQAEVFP"
     gene            complement(4883..4987)
                     /locus_tag="PLB25_13740"
                     /pseudo
     CDS             complement(4883..4987)
                     /locus_tag="PLB25_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429384.1"
                     /note="catalyzes the formation of 3-hydroxybutyryl-CoA
                     from acetoacetyl-CoA in polyhydroxyalkanoate synthesis;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
BASE COUNT         1091 a         1620 c         1427 g          959 t
ORIGIN      
        1 gtttgcgaca ccactgcccg gtgcatcctc gtcaaacttc tgtcgctttt cggcccacaa
       61 aatgatcagc ccacccagga tgaaggtact ggccaccacc accggggtga acaggtgcgc
      121 cttgatggcc ttgccgaaaa gcaggcccag gacaaccgct ggcaaaaagc caatcagcac
      181 gttcagcgca aaacgctgcg cctgccggtc agacggcagg gccaccacgg tatcgcgaat
      241 tttctgccaa tagaccaaaa tcaccgcaaa aatggccccg gtctggatgg caatgtcaaa
      301 caccttgcct ttttcatcgt caaaacccag cagcgcaccg gccaggatca gatgcccggt
      361 gcttgaaatc ggcaaaaact cggtcaaccc ctctaccagc cccatgatgg cggctttcac
      421 cagtaacgca atatccacaa aaactccttg ttgggctggc aattgtgacc gccagcccga
      481 aactgcctat tttcgccagg cccgaatgcc gtctggacgg tgagccggtc ggctgatggc
      541 gcaatggctg cgaaccaggc gtagcgaccc aatttgcgtc gggttcctgg cccacaaggg
      601 ggccatcggc cggcagatcc ggaacacact gggctggccg attctcgctg ctcctgccac
      661 caggctcttg gcgtgccata gacaaccgga aattttggaa gttgcgtcaa tcctttgata
      721 tgccgtcatt gccgcactcg ccggaatcga tggcgttggt tcaatttcag ccctggcggg
      781 aatgacgacg ttgccgccag aacagattca ccttgcccga tgccatcgcc ttcggtacaa
      841 caatgtcggt cgggattgat ccaggaccgg gccagaaggt gatcaagggc gcatcggtca
      901 aatagaatca gcggcagtct tcatcatccg tgctgtcatg agcgccctca ggttgatgtt
      961 gcacggcagg tagctgctta cttcaggaag aaaaacatga tcctcgtcac tggcggtgcc
     1021 ggctttattg gtgccaactt ctgtctcgac tggctggcgc aaagcgatga gccggtcgtc
     1081 aacctcgaca aactcaccta cgcgggcaac ccggaaacct tgcaaagcct gcaaggtgat
     1141 gcccgccaca cgctggtcca cggcgacatt ggcgactctg cgttggtgtc gcaactgttg
     1201 accacgcacc agccacgcgc catcgtcaac tttgccgccg aaagccatgt ggaccgctcc
     1261 attcacggcc caggcgagtt catccacacc aacatcgtcg gtacttatga gcttttggag
     1321 gcggtacgcc aatactggtc tgcgctggca gctcctgaaa aagtagcgtt tcgattcctg
     1381 catgtgtcca ccgacgaggt ctatggcagc ctggccaaag atgacccggc ctttaccgag
     1441 tccagccaca tcgagcccaa cagtccctat tcggccagca aggccgccag cgaccatctg
     1501 gtgcgggcct ggcatcacac ctacggcctg ccggtgctga ccaccaattg ctccaacaac
     1561 tatggcccct accatttccc cgagaagctc atcccgctga tgatcgtcaa cgccttggcc
     1621 ggcaagagcc tgccggtgta tggcgatggc cagcagatcc gcgactggct gtatgtgaag
     1681 gaccattgca gtgccattcg ccgtgtgttg caagccggtc gcccgggcga gacctacaac
     1741 gttggtggct ggaacgaaaa ggccaatctg gagattgtgc ataccgtctg cgccttgctc
     1801 gacgaactgc gcccgcgtgc cgacggcaaa ccctatcggg cgcaaatcac ctatgtcacc
     1861 gaccggcccg gccatgaccg ccgttacgcc attgatgcca gcaagctgga gcgcgaactt
     1921 ggctggaaac cagccgagac tttcgagacc ggcatccgca agaccgtgca gtggtacctg
     1981 gacaaccccg actgggtcgc gcatgtgcaa agcggtgcct accgcgcatg ggtgcaaaag
     2041 cagtacgccg acacgccctg acaaccaacc tgccccattc gccatgaaaa tcctcctctt
     2101 cggcaaaaac ggccaggttg gctgggaact gcaacgcagc ctcagccccc tgggtgaact
     2161 ggtagccctt gacagcgaca gcaccgacct gtgcggcaac ttcactgatc tggccggtct
     2221 ggcgcaaacg gttcgcagcg tacgcccgga tgtgatcgtc aatgcagccg cccatactgc
     2281 ggtggacaag gcggagagcg aacccgatct ggcccgcacc atcaacgccc tcgcccccga
     2341 agtgctggcg cgtgaagcgg cccggctcgg tgcctggctg gtgcactaca gcaccgacta
     2401 tgtgtttgac ggcagcggtg acaagccatg gctggagacc gatgccaccg gccccttgag
     2461 cgtctatggc cgcaacaaac ttgagggcga aaccttgatc gccctgcata acccgcgcca
     2521 cctgattttc cgaactagtt gggtctatgc agcgcgcggc ggcaactttg ccaaaaccat
     2581 gctgcgcctg ggccaggagc gcgaaaagct gaccgtcatc aacgaccaga ttggtgcgcc
     2641 gaccggtgcc gacttgctgg cggatgtcac cgcacatgcg ctgcgccagg tcctgcccgg
     2701 cgcttcacct gaacacatcg ctggtctgta ccacctggtc gccgctggtg aaaccaactg
     2761 gtttgactat gcaaaacacg tgctagccca agcgctgatt gcgcagccag ctatcaaaat
     2821 agtggcgcag cagatcgacc cggtgcccac cagcgcgttc ccgacaccgg cccgcaggcc
     2881 gcacaactcg cggctcaaca ccggcaaact gcagcgcact tttggcctgc gcctgccgcc
     2941 ctggcaacac ggggtcaatc gcatgttggc cgaaattttg taatcttcag gcaagttcac
     3001 catgacaaca cgcaaaggca tcatcctcgc aggtggctcc ggcacccggc tctatccggt
     3061 gacacaggcg gtgagcaagc agctcatgcc gatctatgac aagccgatgg tctattaccc
     3121 cttgagcacg ctgatgctgg ccggcatacg tgaggtgctg atcatctcga caccgcaaga
     3181 cacgccgcgc ttcgccgacc tgctcggcga cggcagccag tggggcatga gcatcagcta
     3241 cgcggtgcag ccctcgcccg atgggctggc gcaggccttc atcattggtc gtgactttgt
     3301 cgccggccag ccgagtgcgc tggtgctggg tgacaatatt ttttacggcc atgacctggt
     3361 caaacagctc gcccatgcca atgcccgcac tgtcggcgcc agcgtgtttg cctaccatgt
     3421 gaccgacccg gatcgctatg gcgtggtggc gtttgacccg caaatgcgcg ccgtcagcat
     3481 tgaagaaaaa ccgacccaac cgcaaagcaa ttacgccgtg accggcctgt atttctatga
     3541 ccagcaggtc tgtgacattg ccgccgacat caagccgtcg cgccgaggcg agctggagat
     3601 caccgatgtc aaccgccgtt acctgcagca aggccagctc aacgtcgaga tcatgggccg
     3661 gggctatgca tggcttgaca ccggcaccca tgacagcctg cttgaagccg ccagtttcat
     3721 cgccacgttg caaaagcgcc agggcctgca ggtggcctgc ccggaagaaa ttgcctacag
     3781 ccagaaatgg attgatgccg cccaaattct taagctgaca accccgctgg caaaaaatgg
     3841 ctatggtcag tatttgctga gcctgctcaa gtgaaagcaa actgcaccgg gtggcggctc
     3901 atgtccgcat tgaccgatgc agccatccac cacaacccag accccacgca ccatgcccta
     3961 caccgtcgtc gccacttcgc tgcctgaagt cctgatcctt gagcccaaag tgtttggcga
     4021 tgccaggggc ttcttttatg agagcttcaa cgcccgcgat ttcacccaat gcaccgggct
     4081 ggacgtgcaa tttgtgcagg acaaccacag caagtcggcc aaaggggtgc tgcgtggcct
     4141 gcactaccag attcagcacc cacagggcaa gctggtgcgg gtaacgcagg gtgaagtatt
     4201 tgacgtggcg gttgatctgc gccgcagctc gcccaaattt ggccgctggg aaggggtcgt
     4261 gctgtcggca gagaacaagc gccagctctg ggtgccgcca ggttttgcgc atggctttgt
     4321 ggtgctgagc gaaagtgccg agtttttgta caagaccacc gattactggt acccggagtt
     4381 cgagcgcagc ctgctctgga gcgaccccac aataggcgtc cagtggccgc ttggcaaccc
     4441 gccgctgctg gcagccaaag accgcgatgc cgccacgctg gagcaggccg aagtgtttcc
     4501 ctgatgcccc cacagcaggg cagcgccgcg ccgggcgagc ccggatgttg cgcacaaaaa
     4561 aaatggccag ccgcgtggtt attcttgcgt cttccagcgg tcagtgcata agccagccac
     4621 cattgaccgg gaacccggta gcgctggcat cagcagcttg gttcgatgtc agccagtcga
     4681 tgatcgagga ttttttcact gctgagcatc ccaaccccgc acgcggggca ggcgttctca
     4741 aaggcacatg ggctaggccg acggcgcaag ctgccagggt tttacctgcc aaatcggcat
     4801 ccagccctta tgcagacagc gttgctagct atatttttga tagttaacac aatgtctgtt
     4861 cgttgtgaga ctgcccaaag gaaccacaag cggcgatcca cttgaacccc agcttgcacg
     4921 ggcgcaggca aactgcgctg tcaatgccgc ccggaccacc ggctactgac gcacctttct
     4981 ggctgatgag tcgatcctgt tgctgacgat caagcagcca ttcgcgctgc cagatcgtcg
     5041 tggtcaggca aaaaaacaat caggcccgca ccaatacata gcggcccggt gctggct
//
LOCUS       DAOSLL010000325         5094 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14635_length_5094_cov_5.464973, whole genome shotgun sequence.
ACCESSION   DAOSLL010000325 DAOSLL010000000
VERSION     DAOSLL010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14635_length_5094_cov_5.464973"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PLB25_13745"
     CDS             complement(<1..366)
                     /locus_tag="PLB25_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42675.1"
                     /translation="MRTCDELGVCNGRHCNDVSACPDYDLIACNSGPCDGDDNAPCPH
                     DVWRCEVPCSPPPQQFEFAQPTYPLASATARSRTSDGKPGYPFAPGIIEGPPVPDGPF
                     DAPWGVRGAVWHRRAFDDAG"
     gene            complement(363..509)
                     /locus_tag="PLB25_13750"
     CDS             complement(363..509)
                     /locus_tag="PLB25_13750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42676.1"
                     /translation="MRTYLIRITMPDGSAGQNHGLYADGFAAVIYALSTFPDAKRISA
                     RRLP"
     gene            complement(519..1736)
                     /locus_tag="PLB25_13755"
     CDS             complement(519..1736)
                     /locus_tag="PLB25_13755"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE42677.1"
                     /translation="MSTSIGTPTKNTASALLHELGIPQSELCRATGLGKATINRICRH
                     GQWPTFNADLAAAAIDGYLVKRGLTPNQVQRLHKALHQKLAPVISNTTEAVPVSPVTE
                     TEKEEPMLLPKQTLSAEARKRFALPFANPFDGEVTSDAEMFVNGEIRFVRESAWQAAI
                     GGRFCAIVGESGSGKTTMLEDLKDKILKEHKPIVFIEPSVLGMEDSDTRGKSIKSAGI
                     QTAIVMTLNPSEGVAQSDEKRARQVKRMLEESTTAGHSHLLVIEEAHSLPIPTLKHLK
                     RLHERMRLGRRPMLGILLLGHPELEGKLNRFDVREVMQRCEVARLCPLGEDLLAYLTF
                     RAASVGRKLDEFISQDGVAELARRLTISAGANSKQVSLLYPLNVNNWMVAALNTAAGF
                     GAPRVDRDIVLAV"
     gene            complement(1793..3589)
                     /locus_tag="PLB25_13760"
     CDS             complement(1793..3589)
                     /locus_tag="PLB25_13760"
                     /inference="COORDINATES: protein motif:HMM:NF012870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HOE42678.1"
                     /translation="MDPIRTEIIHAAARELAAASHGDKGQIIARAASTAGLSVNRTHT
                     LVRHASGMLGLAKPRKRRSDAGETAISEDDLVKIAGAMRSAQRNGKWMLSAQDAIEML
                     DANGQLSATLSPGRVHHIMHQRGMHPVQLAAPAPAVRMRLEHPNAVWQIDASVCVLYY
                     TPAGEMLLLEEDGVHYKNKLANYTRVMNELLTRFVGTDPASGCIATRFYSGGETTENA
                     LAFLMWMMTARHDLAGRPMPFHGVPLALYSDQGSCFKSGPFANFCAAMGIKQTRHAPR
                     NSRATGHVERANDLEERGLESRLRFMDPKTITMASLNDMAELWMHAFNGTRTMRRHGM
                     TRYAAWSLITPEQLRLAPSMDIMQALPATLAKTRQVSDDMQVSFAFKDLGRHDYDVRY
                     VPGISPRSQVYVSVNPFEYPAVRVGAVDRDSGEIVWHQVEPVQRDRLGYDVAAPIMGQ
                     SYHAMPETTSDVWRKKIAAQAYATASGPATPDQADIARRSNTAAYLGQFDPLADLKAV
                     QLPTYLPRKGQAFEAAAPSVQAPRLSVAEACKRIKLALGDAYDAGTYAWLTERHGSNG
                     VPEDAVQALIAQHRQAVVPTEPVRLRAVGGGV"
     gene            complement(3612..4703)
                     /locus_tag="PLB25_13765"
     CDS             complement(3612..4703)
                     /locus_tag="PLB25_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42679.1"
                     /translation="MSRTPNAIAQDTPIQFDHALLAQDAQAARDLAQMKADYAEGRDL
                     ANQLLGQVQMADSLSKFTMTVSVSKLAYVKEHKLYKALKGKKTADGQQFSGAFPIESA
                     DKTADGQQFSGTWEEYCALLGRSHQQVDEEIRNLKTFGEAALESMSAMGIGYRELRQY
                     RKLPADQQMALIEVAKVGDKEGFVELAEEIIAKHAKEKEAHAAALAEAQANLAAKQRA
                     LDASSATIANLHEQLGGEYEPRPGSIAKSEQEENLLEEISAITLECGTMMRRLFLAAD
                     TTFDAPVGEGVKNLARQAVEYLCQELASISVEFGIKVDFENLVNPPWMSEAMLAAMQS
                     KIDERTARDAAKAKKRASGKPGTPADTQP"
     gene            complement(4740..5042)
                     /locus_tag="PLB25_13770"
     CDS             complement(4740..5042)
                     /locus_tag="PLB25_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42680.1"
                     /translation="MNTDSNQTLRVLNLAEVLCGFVVLGISNKSLAALTQTTPVQVSK
                     DIAYLIAKGWARKDDKTGHFHPTPEMTRVFQRVATDLERAQQEVTDITHRYIRPAQ"
BASE COUNT          818 a         1491 c         1679 g         1106 t
ORIGIN      
        1 cccggcatca tcgaaggccc gccggtgcca gacggcccct ctgacgcccc atggcgcgtc
       61 aaaggggccg tctggcaccg gcgggccttc gatgatgccg ggggcaaacg ggtagccggg
      121 tttgccgtcg ctggtgcgcg atctggcggt ggcgctggca agcgggtagg tgggctgggc
      181 aaattcaaac tgctgtggcg gcgggctgca gggcacctcg caccgccaaa catcatgtgg
      241 gcagggtgcg ttgtcgtcac cgtcacatgg gccgctgttg caggcaatca ggtcgtagtc
      301 agggcaggcg ctgacatcgt tgcaatggcg gccattgcac acaccaagtt cgtcacaagt
      361 tctcatggca gtctccgggc gctgatgcgt ttggcatcgg gaaaagtgga cagggcatag
      421 atgacggctg caaagccgtc ggcatacagg ccgtggttct ggccggctga gccgtctggc
      481 atggtgatgc ggatcaggta ggtgcgcatg ggatcgcctc aaacagccag cacaatgtca
      541 cggtccacac gcggggcacc aaagccagcg gcggtgttca gcgcggccac catccagttg
      601 ttgacgttca gcgggtacag caggctgacc tgtttgctgt tggccccggc gctgatggtc
      661 aggcgcctgg ccagctcggc cacaccgtct tggctgatga actcgtcaag cttgcggccc
      721 acactggcgg cacgaaacgt caggtaggcc agcaggtctt cacccagcgg gcacaggcgg
      781 gccacctcgc agcgctgcat cacctcacgc acgtcaaagc ggttgagctt gccttccagc
      841 tcggggtgcc ccagcagcaa aatgcccagc atggggcggc gacccaggcg catgcgttcg
      901 tgcagccgct tcagatgctt cagggtgggg atgggcagcg aatgtgcctc ttcaatcacc
      961 agcaggtgcg aatgcccggc ggtggtgctt tcttccagca tgcgcttgac ctggcgggcg
     1021 cgcttttcgt ccgactgggc cacgccctca ctcgggttga gcgtcatcac aatggcggtc
     1081 tgaatgcccg cactcttgat gcttttgccc cgcgtgtcgc tgtcttccat gcccagcacg
     1141 ctgggctcta tgaacacaat gggcttgtgt tccttgagta ttttgtcttt caggtcttcc
     1201 agcatggtgg tcttgccaga gccgctttcg cccacaatgg cgcaaaagcg cccgccaatg
     1261 gcagcctgcc aggccgattc gcgcacaaag cgaatctcgc cattgacaaa catctcggca
     1321 tcgctggtca cctcgccgtc aaacgggttg gcaaatggca gggcaaagcg cttgcgcgcc
     1381 tccgcactca gggtttgctt gggtagtaac atcggttctt ccttttcggt ttcagttacg
     1441 ggactcacgg gaacggcctc agtggtgttc gagatcactg gggccaactt ttgatgcagc
     1501 gctttatgca ggcgctgcac ttggttgggg gtcaggccac gcttgaccag gtagccatca
     1561 atggccgcag cagccaggtc agcattgaag gttggccact ggccatggcg gcagatgcgg
     1621 ttgatggtcg ccttgcccag accagtagcc cggcacagct cagactgcgg aatgcccagc
     1681 tcgtgcaaca gggcgctggc ggtgtttttg gtcggtgtgc ctatgctggt gctcatattg
     1741 ctgtcaggta tgttttggaa cggctgtaag gcgttttgat gggtgttttg gctcataccc
     1801 caccccccac agcccgcaag cgcaccggct ctgtcggcac cacggcctgg cggtgctggg
     1861 ctatcagcgc ctgcaccgcg tcttcaggca caccattgct gccatggcgc tcggtcagcc
     1921 aggcgtaggt gcctgcgtca taggcatcac ccagcgccag tttgatgcgt ttgcaggcct
     1981 cggccacgct caggcggggg gcttgcacgc tgggggcagc ggcctcaaag gcttgcccct
     2041 tgcgtggcag gtaggtgggc agttgcaccg ccttcaggtc agccagcggg tcaaactggc
     2101 ccaggtaggc ggcggtgttg ctgcgcctgg caatgtcggc ctggtctggc gtggccgggc
     2161 cgctggccgt ggcataggct tgtgcggcaa tcttcttgcg ccacacatca gaggtggtct
     2221 cgggcatggc gtggtaactt tggcccatga tgggggcggc cacgtcatag cccaggcggt
     2281 cgcgctgcac cggttctacc tggtgccaca caatctcgcc gctgtctcgg tccaccgcac
     2341 ccacacgcac ggcggggtat tcaaacgggt tgacgctgac atacacctgg gagcgcggcg
     2401 agatgccggg cacatagcgc acgtcatagt catggcggcc caggtccttg aaggcaaagc
     2461 tcacctgcat gtcatcgctc acctggcgcg tcttggccag tgtggcgggc agcgcctgca
     2521 tgatgtccat gctgggggcc aggcgcagtt gctcgggggt gatcaggctc caggcggcgt
     2581 agcgcgtcat gccgtggcgg cgcatggtgc gggtgccgtt aaaggcgtgc atccacagct
     2641 cggccatgtc gttcaggctg gccatggtga tggttttggg gtccatgaag cgcaggcggc
     2701 tttccagccc gcgttcttcc aggtcattgg cgcgctctac atggccggtg gcgcggctgt
     2761 tgcgcggggc gtggcgggtt tgcttgatgc ccatggcggc gcaaaagttg gcaaacgggc
     2821 cagacttgaa gcaactgccc tggtcgctgt acagcgccag tggcacaccg tgaaaaggca
     2881 tcggccggcc agccaggtcg tggcgcgcgg tcatcatcca catcagaaag gccagcgcgt
     2941 tctcagtggt ttcgccgccg ctgtaaaagc gcgtggcaat gcagccactg gcggggtcgg
     3001 tgccgacaaa gcgggtcagc agctcgttca tcacccgggt gtagttggcc agcttgtttt
     3061 tgtagtgcac gccgtcttct tccagcagca gcatttcacc ggccggggtg tagtacagca
     3121 cgcacacgct ggcgtctatt tgccaaacgg cgttggggtg ctccaggcgc atgcgcaccg
     3181 ccggggccgg ggcggccaac tgcaccgggt gcatgccgcg ctggtgcatg atgtggtgaa
     3241 cacgcccagg gcttaaagtg gcactaagct ggccattggc gtccagcatc tcaatggcat
     3301 cttgcgcgct gagcatccat ttgccattgc gctgggcgct gcgcatggca ccggcaattt
     3361 ttaccaggtc gtcttcactg atggcggttt caccggcatc agagcggcgc ttgcgtggct
     3421 tggccaggcc cagcatgcca ctggcatggc gcaccagggt gtgggtgcgg ttgaccgaca
     3481 ggcccgccgt gctggcggcg cgggcaatga tctggccctt gtcaccgtgg ctggcagcgg
     3541 ccagctcgcg ggcagcggcg tgaatgattt cagtgcggat cgggtccatg gtgcggcgca
     3601 ggtggtgggg tttacggctg ggtgtcggct ggcgtgccgg gcttgccgct ggcgcgcttt
     3661 ttggctttgg cggcatcgcg ggcggtgcgc tcgtcgatct tgctttgcat ggcggctagc
     3721 atggcctcgc tcatccaggg cgggttcacc aggttttcaa agtcaacctt gatgccaaat
     3781 tcaaccgata tgctggccag ctcctggcac aggtactcaa ccgcctggcg cgccaggttt
     3841 ttcacgcctt cgcccaccgg cgcgtcaaag gtggtgtcag cggccagaaa caggcggcgc
     3901 atcatggtgc cgcattcaag tgtgatggcg ctgatttctt ccagcaggtt ttcttcttgt
     3961 tcgcttttgg caatgctgcc ggggcggggt tcatattcgc caccgagctg ctcatgcagg
     4021 ttggcaatgg tggcgctgct ggcatcaagg gcgcgctgct tggcggccag gttggcctgc
     4081 gcttcggcca gggcggcggc gtgggcttct ttttctttgg cgtgtttggc aatgatttct
     4141 tcggccaact ccacaaagcc ttctttgtca ccgaccttgg cgacttcgat cagggccatc
     4201 tgctggtcgg cgggcagttt gcggtactgg cgcagttcgc ggtagccaat acccatggct
     4261 gacatggatt cgagggcggc ctcgccaaag gttttaaggt tgcggatttc ttcgtcaact
     4321 tgctgatgtg accgccccaa cagtgcgcaa tactcctccc atgtgcctga aaactgctga
     4381 ccgtcagcag ttttatcagc actctctata gggaacgcac ctgaaaactg ctgaccgtca
     4441 gcagttttct tgcctttaag ggctttgtac aacttgtgtt ctttgacata agcgagtttg
     4501 ctgacactga ccgtcatggt gaacttgctc agcgaatccg ccatctgcac ctgccccagc
     4561 aactggttgg ccaggtcacg gccttcggcg taatcggctt tcatttgggc caggtcgcgc
     4621 gcggcttgcg catcttgcgc cagtagcgcg tggtcaaact ggataggggt gtcttgggca
     4681 atggcgttgg gagtgcgtga catgtttctg gctttggcgt tggggttaaa aatgcagggt
     4741 tactgcgcgg ggcggatgta gcggtgcgtg atgtcagtca cctcctgctg cgcccgctca
     4801 aggtctgtgg ccacgcgctg aaagactcgt gtcatctcag gcgttgggtg aaagtggccg
     4861 gttttgtcgt ccttgcgcgc ccagcctttg gcaatcaggt aggcaatgtc cttgctcacc
     4921 tgcaccgggg tggtttgggt cagtgcggcc agcgacttgt tgctgatgcc cagcaccaca
     4981 aagccgcaca gcacctcagc caggttgagc acgcgcaggg tctggttgct gtcagtgttc
     5041 atgctggtct gccttgcgcc ggttaagcaa tgtggtcggc ccttcgatgc tggc
//
LOCUS       DAOSLL010000326         5089 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14652_length_5089_cov_4.336114, whole genome shotgun sequence.
ACCESSION   DAOSLL010000326 DAOSLL010000000
VERSION     DAOSLL010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5089
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14652_length_5089_cov_4.336114"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(195..>681)
                     /locus_tag="PLB25_13775"
     CDS             complement(195..>681)
                     /locus_tag="PLB25_13775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HOE42681.1"
                     /translation="KTVSKLQLALASRAQDATTSEASAGSDGASLEATVRQLRQLLAS
                     IDGPKPAPAMPLRHIAASVGATLRLVPVDEVVYFEAADKYVRVLTREREYLIRTPLKD
                     LLPQLDASVFWQIHRGTVVNNRFIETMLRDEAGKAFVTLVGLPGRLAVSRLYVHLFRP
                     M"
     assembly_gap    682..691
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<692..1033)
                     /locus_tag="PLB25_13780"
     CDS             complement(<692..1033)
                     /locus_tag="PLB25_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE42682.1"
                     /translation="MTASATALIAEDEPLLAAALQAELARAWPALQVLAVLGDGQAAL
                     SQALLLQPDVLFFDIRMPGLSGLEAATELVDAWDSRQKPFPALVFVTAYEQYAVQAFE
                     AQAVDYLLKPVQ"
     gene            complement(1046..2077)
                     /locus_tag="PLB25_13785"
     CDS             complement(1046..2077)
                     /locus_tag="PLB25_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HOE42683.1"
                     /translation="MKQKPTLKRALQIVAFNTVIALGITAFMQQGLWINLVYSQCIGL
                     SIWALIDFGQYWLVSDLQLQWRRLFLIAPVGAALGYLLGTAAAQRFFPGHDFHHWSSH
                     PNQALGFLIISLVAGGISTYFFMSREQLALARADMARTQREAEAAQRQAAQAQLTLLQ
                     SQLEPHMLFNTLANLRALISLDPERAQTMLDHMVAYLRATLGASRATLHPLQTEFDRL
                     GDYLELIKVRMGARLHYTLTLPDDLAGLNVPPLLLQPLVENAIKHGLEPKVAGGGVLV
                     SARQQGSQITLEVQDTGVGLAAGAAVADGFGLAQLRERLQTVYGPTATMNLVADSAGG
                     TRAIVTFFI"
     gene            3002..3262
                     /locus_tag="PLB25_13790"
     CDS             3002..3262
                     /locus_tag="PLB25_13790"
                     /inference="COORDINATES: protein motif:HMM:NF016268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HOE42684.1"
                     /translation="MWAGRKNWRKHGVSLEEAASVFSDALAYTFADPDHSVGEQRLLT
                     FGFSQFGQLLAVVHSGQGRAIRIISARNATRNERGICEQNQR"
     gene            complement(3976..>5089)
                     /locus_tag="PLB25_13795"
     CDS             complement(3976..>5089)
                     /locus_tag="PLB25_13795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOE42685.1"
                     /translation="QVSTQVAMAELLMSGCTTSSDHLYIYPNGVRLDDSIEAAQQIGM
                     RFVATRGSMSVGISQGGLPPDSVVETEDLILKDTQRLIETWHDSRHGAMVNVAVAPCS
                     PFSVSRDLMRESAALARKYRVRLHTHLAENDHDLAYSQEKFNCTPAQYAQDLGWLGPD
                     VWHAHCVRLDDVGIHLFAASRTGVAHCPCSNMRLASGIAPVRKMLDAGVPVGLGVDGS
                     ASNDAAHLVHEARQALLLARVGRAMRAAETRSDSQGRQRSFFGCDLGPAEMTARDALT
                     MATRGGAQVLGRSDIGHLAPGMCADLALFDLNTLAFAGGAVHDPVGAVLLCASPQAAY
                     TIVNGRVLVRQAQLTTLELGPLIERHNKLAMQLARV"
BASE COUNT         1042 a         1498 c         1528 g         1011 t
ORIGIN      
        1 ggcagtagcc ttgctggtgt cagaactgct gatggacgaa ggctacccct acaacgccta
       61 tcttgaacgg gttcgaaccg ggctgaagtc tgccgaataa gcacggagca accccatcga
      121 ctgccgtcag cttgcagcgg cggcagcggt ggatggcttg ccggggccgc aggtttgttg
      181 ggcggtggcc caggctacat ggggcgaaac aaatgcacgt agagccgact gaccgccagc
      241 cgcccaggca aaccgaccag cgtcacaaac gccttgccag cttcgtcacg caacatcgtc
      301 tcaatgaagc ggttgttgac caccgtaccc cggtggattt gccagaaaac gctggcatcg
      361 agttgtggca gcaagtcttt caacggggtg cgaatcaggt actcgcgctc gcgcgtcagc
      421 acccgcacgt acttgtcggc ggcctcgaaa tagaccactt catcgactgg caccagacgc
      481 aaggtggcac cgacactggc cgcaatgtgc ctgagcggca tggcgggcgc tggttttggc
      541 ccatcaatgc ttgccagaag ctgccgcagt tgcctcacgg tggcttcaag tgatgcgccg
      601 tcagaaccag cggatgcttc tgaagttgta gcgtcctgcg cacggctggc aagggccaac
      661 tgcagttttg acactgtttt tnnnnnnnnn nctgcaccgg tttgagcagg tagtcgaccg
      721 cctgcgcctc aaacgcctgc accgcgtact gctcataggc ggtgacaaat accagcgccg
      781 gaaacggttt ttgtcggctg tcccaggcat cgaccaattc ggtggctgcc tccaggccac
      841 tcaggccggg catgcgaatg tcaaaaaaca gcacatcagg ttgcagcaac agcgcctgcg
      901 acagcgccgc ctggccgtca cccagcaccg ccagcacctg caatgctggc caggcgcggg
      961 ccaattcggc ctgcagcgca gccgccagca ggggttcgtc ttcagcaatc agggccgtgg
     1021 cagaagctgt catgatcatc gcaacttata tgaaaaaagt gactatagcc cttgtcccac
     1081 cagcgctatc agctactaaa ttcatagttg ccgtggggcc atagacggtt tgcaggcgtt
     1141 cgcgcaactg ggccagtccg aagccatcag cgaccgcagc acccgctgcc agcccgacac
     1201 cggtgtcttg cacctccagc gtgatctggc tgccctgctg gcgtgccgac accaatacgc
     1261 caccgcccgc caccttgggt tcaaggccgt gtttgatggc gttttccacc agcggttgca
     1321 gcagcagcgg tggcacattc aggccggcca gatcgtctgg cagcgtcagc gtgtaatgca
     1381 ggcgcgctcc catgcgcacc ttgatcagtt caaggtagtc accaaggcgg tcaaattcgg
     1441 tttgcagcgg gtgcagggtg gcacgcgacg cgcccagggt cgcgcgcaaa taggccacca
     1501 tgtggtcaag catagtttgc gcccgctctg ggtccaggct gatcagggca cgcagattgg
     1561 ccagtgtgtt gaacagcatg tgcggctcca gttggctttg cagcagcgtg agctgcgctt
     1621 gtgccgcctg ccgctgcgcc gcttcggcct cgcgctgggt gcgggccatg tcggcacggg
     1681 ccagtgccag ttgctcgcgg ctcatgaaaa aataggtgct gatgccaccc gccaccaggc
     1741 tgatgatcaa aaagcccagc gcctggttgg gatgactgga ccaatggtgg aagtcgtgac
     1801 ccggaaaaaa gcgctgcgcg gcggcggtgc cgagcaaata acccagcgcc gcgccaactg
     1861 gcgcgatcag gaacaggcga cgccactgaa gctgcaggtc gctcaccagc cagtattgac
     1921 caaagtcgat cagcgcccag atgctcaaac cgatgcactg cgagtacacc aggttgatcc
     1981 acaggccctg ctgcatgaat gcagtgatgc ccaaggcgat cacggtgttg aaagccacga
     2041 tctgcagggc gcgtttgagg gttggttttt gtttcatcaa ggcgcaattg ttcacgatca
     2101 gcactcggct tggtcatttt tgcgatgaat cagcgcctgg ttggcgcgaa acgcagcctg
     2161 ctggcgtaaa agctcaggcc tatgccgcgc tgatgcaaaa aagggtgcgg cgatcacagg
     2221 cgcaaggccg ggtcagggcg cttgggcact cactgcacaa gcggtttgca acgaagcatt
     2281 gcgcttgtca gtgcgttcga actgttcaag ttatttttgc ttgaccccta tgccgaacga
     2341 gccggaatcg atgagccttc gtcgcgaggg cgcgcctcct acacaaacag tcggcgccca
     2401 tttgaatttg taggagtcgc gccctcgcga cgaatgactt tgaatgtttg agttcatcgc
     2461 atgatgtgta aagacttcac ttgttagagc ctaggcattc tcccagacaa gctgggaaaa
     2521 tgaggttcgc ctgaggcgaa cagcctgtgg tgaagttgtg tcgacaaaga tcgacaacat
     2581 gttggtaagt ttgatgcgca tagcgcctaa gggcacgcgc ttgctgcagg cgcatgcgcc
     2641 agtggcacga atagctgttg accggggtgt tcagttcgct ggctttgaag cccaggccca
     2701 gagcaagcct tgagcccgcg ctggcgcatc ccatagaaaa cgctcgggcc tgctatcagt
     2761 gcgttttcag aaatattcaa aagtaatagc tgctcacgca atacccaaaa gggctggagc
     2821 cgtttcttgc ataaactttt cacacggtcg ccggtgtcgg cagcgggaca gtgcaggcca
     2881 cgcaaagtgg ctgaaaacgt tggaggttgc tggcccaaca tgctgacaca ggattgccgt
     2941 ctgcctggtg acgctccaag ccgcacaagc tgttgcattt gcccaacgcg tcagcaatgc
     3001 agtgtgggct ggcagaaaaa attggcgcaa gcatggtgtt tcgttggaag aggccgcgtc
     3061 agtgttttca gacgcgctgg cgtacacttt tgccgaccct gaccactcgg tgggtgagca
     3121 gcggctactg actttcggtt tctcgcaatt tggacagctt ttggcagttg ttcactccgg
     3181 gcaagggcga gctatccgaa tcatcagtgc ccgtaacgca actcgaaatg aacgaggtat
     3241 atgtgagcaa aaccaaagat gacatgcgcg atgaatatcg gcgtgaagac ttggttaagg
     3301 gtgtgtgagg caaatacttc gcgcgggtag cgaaagggac caatcttgtt tttctggacg
     3361 ataaagttgc caaatcgttt tcaacaggcg ctgctgtgaa ccaagcgttg ctagggctgc
     3421 tcgcgcttac cgagcaaacg acgcgtataa atgatcgctc aacccagacg acccgcaagc
     3481 gggccgcagt ttagcttcga ggttggatga tcccggatca ggccaaaaac gcctgccttg
     3541 ccgacactga cagtatcttt ccccatcgca ccggctgcaa gggtcttggg aaccccaata
     3601 ccggcagagc cgatcaatca agccatcatt gcaatggctg cgcgcccaaa ccgggcatct
     3661 tgaggcagcc aaatcagccg gtttgagctg ctttacgcca agcgatgcca cagcaaccgc
     3721 gtaggcactg gtgcgctggg tttgatccct ggtgttatcc gatggcaaag ccctcgcggc
     3781 tattgcgcac cgactgcgca attgcaagac cagatcaaaa acgtgctgaa gtttccttca
     3841 cgctgtcagc ggctgagttt gaggaagcgc tgccaatcat cggggtcatt gttggcccaa
     3901 aagacccgtt ggcgggtagc tgactgcgcc attgagcgtt cttggccaga acttctgaaa
     3961 attgaccccg gacgctcaaa ctctcgccag ttgcatcgcc agcttgttat gtcgctcaat
     4021 cagcggcccc agttccagcg tggtgagctg tgcttggcgc accagtaccc ggccattgac
     4081 gatggtgtaa gccgcttgcg ggctggcgca cagcagcacc gcaccgacag ggtcatgcac
     4141 cgcgccaccg gcaaaggcca gcgtgttcag gtcaaacaag gccaggtcgg cacacatgcc
     4201 cggtgccagg tggccaatgt cgctgcgccc caatacctgg gcaccgccac gggttgccat
     4261 cgtcagcgca tcgcgggcgg tcatttcggc tggtcccagg tcgcaaccaa aaaagcttcg
     4321 ctgccgtccc tggctgtcac tgcgggtttc tgctgcccgc atggcgcggc caacgcgggc
     4381 cagcagcagc gcctggcgag cctcatggac caggtgggca gcgtcgttgc tggcgctgcc
     4441 atcgaccccc agcccgaccg gcacacccgc atcgagcatt ttgcgaactg gcgcaatgcc
     4501 cgaggccagt cgcatgttgc tgcacgggca atgtgccacg ccggtgcggc tggcggcaaa
     4561 cagatggatg ccgacatcgt cgagccgcac acagtgggcg tgccacacgt cgggcccaag
     4621 ccagcccagg tcctgggcat actgggccgg cgtgcagttg aatttttcct gcgagtaggc
     4681 caggtcatgg tcgttttcgg ccagatgagt gtgcaggcgc acccggtact ttcgggccaa
     4741 tgcggccgat tcgcgcatca ggtcgcggct gacgctgaat ggcgaacagg gcgcaaccgc
     4801 cacgttcacc atggccccgt ggcggctgtc gtgccaggtt tcgattagcc gctgcgtgtc
     4861 tttcaggatc agatcttcgg tttccaccac gctgtccggt ggcaggccac cctggcttat
     4921 gcccacgctc atgctgcctc tggtggcgac aaaacgcatg ccgatctgct gcgcggcctc
     4981 aatgctgtca tccagtcgca cgccgttggg gtaaatgtag aggtggtcgc tgctggtggt
     5041 gcagccgctc atcagcagtt cggccattgc cacctgggtg ctgacctgc
//
LOCUS       DAOSLL010000327         5078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14700_length_5078_cov_3.908620, whole genome shotgun sequence.
ACCESSION   DAOSLL010000327 DAOSLL010000000
VERSION     DAOSLL010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14700_length_5078_cov_3.908620"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..369)
                     /locus_tag="PLB25_13800"
     CDS             complement(112..369)
                     /locus_tag="PLB25_13800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42686.1"
                     /translation="MNAVWQVQAAKSRFGDMIKTSRPPVIARHGRAVAQVLATRPGER
                     AASAAPPDAFEAFVACLQSAPRADRRAVPLRRSRKLALALG"
     gene            complement(416..1780)
                     /locus_tag="PLB25_13805"
     CDS             complement(416..1780)
                     /locus_tag="PLB25_13805"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HOE42687.1"
                     /translation="MKPIQVGLLGIGVVGSGTFNVLARNQQEIMRRAGRGIEITMVAD
                     LNVERAQSLVGPNVQVVSDARAVIANPDIDIVIELIGGYGIARTLVMEAIAAGKHVVT
                     ANKALLAVHGTEIFAAASAKGVMVAFEAAVAGGIPIIKALREGLTANRIQWIAGIING
                     TTNFILSEMRDRGIDFAVALKAAQRLGYAEADPTFDIEGVDAAHKATIMSAIAFGIPV
                     QFDKAYVEGITKLGAADIRYAEQPGYRIKLLGITKRRAGDVKGTREGIELRVHPCLIP
                     AKRLIANVEGAMNAVVVQGDAVGTTLYYGKGAGSEPTASAVIADLVDITRLHTADAAH
                     RVPHLAFQPNAMSQLVVLPMSEVVTSYYLRLRVADETGVLAKVTGILATAGISIDAVL
                     QRQADEISGESSVAGQPVVAQTDVIILTHDCMEASMNAAIALMQALPTVLEPITRIRK
                     EELA"
     gene            complement(1857..3083)
                     /locus_tag="PLB25_13810"
     CDS             complement(1857..3083)
                     /locus_tag="PLB25_13810"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOE42688.1"
                     /translation="MKTIRKSAKLSNVLYDIRGPIMDAARQMEDEGQKIIKLNLGNLA
                     VFGFDAPEEIQQDMIRNLPNSAGYSDSKGIFAARKAVMHETQKQGVPGVTLDDIYLGN
                     GASELITMATNALLDNGDELLLPMPDYPLWTASVSLSGGTPVHYLCDEGNGWMPDLAD
                     IRSKVSPRTKGIVVINPNNPTGVLYSVELLQGIVSIAREFGLVILADEVYDKVLYDGA
                     KHTALACLSSDVLTLTFNSLSKSYRSCGYRAGWMVVSGDKKSAVDYIEGLNMLSNMKL
                     CSNVPGQWAIQTALGGYQSINDLTCAGGRLRRQRDLAYELITAIPGVSCVMPQAALYI
                     FPKLDPIIYPITDDRQFFLELLRETRVMLVQGSGFNWPQPDHFRIVFLPHEDDLREAI
                     GRIARFLESYRKRRSN"
     gene            3197..3565
                     /locus_tag="PLB25_13815"
     CDS             3197..3565
                     /locus_tag="PLB25_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HOE42689.1"
                     /translation="MKYQPDIISVQSIHSYGPGWIQVGATKLTHSVILGSRGERLDWH
                     CERFEDLTPAHFEQLAQMQTELVIFGSGPRLRFVAPRLSCSLIERQIGMETMDTHAAC
                     RTYNILASEGRCIAVALLMQ"
     gene            3662..4147
                     /locus_tag="PLB25_13820"
     CDS             3662..4147
                     /locus_tag="PLB25_13820"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOE42690.1"
                     /translation="MAIVVNKPIPEFESNATGGVRVTNTSHLGRIVVLYFYPKDNTPG
                     CTTEAMEFRDKYKEFVKAGATVFGVSRDNMRSHDEFKQKLELPFELIADTEEKMCHMF
                     GVVKNKIMYGKKVKGIERSTFLIGADGCLKDEWRGLKVPGHVDEVLESVKELKKSAVL
                     A"
     gene            4549..5046
                     /locus_tag="PLB25_13825"
     CDS             4549..5046
                     /locus_tag="PLB25_13825"
                     /inference="COORDINATES: protein motif:HMM:NF025022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOE42691.1"
                     /translation="MPLPSPPSKRAALLSLKDLDAPLAKSKTRADRVQTPAAAPATPA
                     ALSAATPPLIATVTLPLSQSATAEQMTVAPQQSVAKALPRVKSKSRQTRGPGKLFVLD
                     TNVLLHDPTCLFRFEEHDIFLPMIVLEELDAHKKGMTEVARNGRQTSRSLDALAEHPV
                     AMSAP"
BASE COUNT         1039 a         1533 c         1420 g         1086 t
ORIGIN      
        1 ggccaaaacg gccaaaacgg cgctgctgcc gaaccgttgc aacacctgcg catctggcgc
       61 ttttcgtgtc agtccgaaga ccacaccggt gtctggcaac cactgcatgg cttagcccag
      121 tgccagcgcc aacttgcggc tgcggcgcag cggcactgca cgccggtcag cacgcggcgc
      181 ggattgcaga caggcgacaa aagcctcgaa agcatcgggt ggcgcggctg aagctgcccg
      241 ctcgcccggc cgggttgcca gcacctgcgc cacagcacgg ccatgacgtg cgatgaccgg
      301 cgggcgactg gtcttaatca tgtcgccgaa gcgacttttc gccgcttgaa cctgccacac
      361 agcattcatg caagcacctt tttttcacct ggtctggcca gaatagctgg gcgtatcagg
      421 ccagctcttc cttgcggatg cgggtaattg gctccagcac cgtgggcagc gcctgcatca
      481 gcgcgatggc ggcgttcatg ctggcttcca tgcagtcgtg cgtcaggatg atcacgtcgg
      541 tctgagcgac caccggctgc ccggcaacgc tggattcacc gctgatctca tcggcctggc
      601 gctgcagcac ggcatcaatg ctgatgccag ccgttgccaa aataccggta actttggcca
      661 gcacgccggt ttcatcggcc acccgcaggc gcaggtaata gctggtgacc acttcgctca
      721 tcggcagcac caccagttgg ctcatggcgt tgggctgaaa tgccaggtgc ggcacgcggt
      781 gtgcggcatc ggcggtgtgc agccgggtga tatcaaccag atcggctatc acggcactgg
      841 cggtcggctc ggagccggcc cctttgccgt aatacagcgt ggtgccaacc gcgtcgccct
      901 gcaccaccac cgcattcatg gcaccttcga cattggcaat caggcgcttg gccgggatca
      961 ggcaagggtg cacgcgcagc tcgatgcctt cacgcgtgcc cttgacgtcg cctgcccgac
     1021 gtttggtgat gccaagcagc ttgatgcggt agccgggctg ttcggcgtac ctgatgtcgg
     1081 ccgcgccaag tttggtgatg ccttccacgt aggccttgtc aaattgcacc gggatgccaa
     1141 aggcaatcgc gctcatgatg gtggccttgt gggcggcatc aaccccttcg atgtcaaagg
     1201 tcgggtcggc ttcggcataa cccagccgct gcgctgcttt gagcgccacg gcgaaatcta
     1261 ttcccctgtc gcgcatttca ctcaggatga agttggtggt gccgttgatg atgcccgcaa
     1321 tccactggat gcggttggcg gtcaggccct cacgcagcgc cttgatgatc ggtatgccac
     1381 cggccactgc cgcctcaaaa gccaccatca cgcccttggc cgatgcggcg gcaaaaatct
     1441 cggtgccatg caccgccagc agcgccttgt tggcggtcac cacgtgtttg cctgccgcaa
     1501 tggcttccat caccagcgtc ctggcaatgc cgtagccgcc aatcagctcg atcacaatgt
     1561 caatgtcagg gttggcaatg acggcgcgcg cgtcgctgac gacttgcaca ttcggcccga
     1621 ccaggctttg tgcccgctcg acattgaggt cagccaccat ggtgatctca atgccacggc
     1681 cagcgcggcg catgatttcc tgctggttgc gtgccagcac attgaacgtc ccagagccca
     1741 ccacaccgat gcccagcagg ccgacttgaa ttggtttcat catttggaga tatttggtta
     1801 aacaggcttg tagcccttgc tgcgtatgcg caagcagcta tcaaattaat attgaatcag
     1861 ttgctgcggc gctttcggta actttccaga aacctggcaa tgcggccaat ggcctcacgc
     1921 aaatcgtctt catgcggcaa aaacacaatg cggaaatggt ccggctgcgg ccagttgaag
     1981 ccgctgccct gcaccagcat gacgcgggtt tcgcgcagca actccagaaa gaactggcga
     2041 tcatcggtga tggggtaaat gatcgggtcg agcttgggga aaatgtacag cgcggcctgt
     2101 ggcatgacgc agctcacccc cggtatcgcg gtgatcagtt cataagccag atcacgctgg
     2161 cggcgcaagc gtccgcctgc acaggtcagg tcgttgatgc tctggtagcc tcccagagcg
     2221 gtctgaatcg cccactgtcc tggcacgttg gagcacagct tcatgttaga gagcatgttc
     2281 aggccttcga tgtagtcaac tgccgacttc ttgtcgcccg agaccaccat ccagccggcc
     2341 cggtaaccac aggaacggta gcttttcgac agcgagttga aggtcagtgt caacacatca
     2401 ctcgacaggc aggccagggc agtgtgtttg gcaccgtcat acaacacttt gtcatagacc
     2461 tcatcggcga ggatcaccaa gccaaattcc cgcgcaatgc tgacgatgcc ctgaagcaac
     2521 tcgaccgaat acagcacgcc ggtcgggtta ttcggattga tcaccacgat gcctttggtg
     2581 cgcgggctga ctttggaacg gatgtcggcc aggtcgggca tccagccatt gccctcgtca
     2641 cacaggtaat gcaccggcgt gccgcctgac aggctgaccg atgccgtcca cagcgggtag
     2701 tcgggcatgg gcagcagcag ttcgtcgccg ttatcgagca gcgcattggt ggccatggtg
     2761 atgagctcgc tggcaccatt gcccagatag atgtcgtcaa gcgtgacacc cggcacgcct
     2821 tgcttctggg tctcatgcat caccgccttg cgcgctgcga aaataccctt gctgtcagag
     2881 tagcccgccg agttgggcaa gttgcgaatc atgtcttgct gaatttcttc aggcgcgtca
     2941 aagccaaaca ccgccagatt gcccagattg agcttgatga tcttttggcc ctcgtcctcc
     3001 atctggcgtg ccgcgtccat gatcggccca cggatgtcgt aaagcacgtt ggacagtttg
     3061 gctgatttgc gtatggtctt caaaatccct ccagcaatgg ctgcaattgg caaaaactat
     3121 aatttgaaca cagttttttt tgcctgttgc gctctggcga cgcccgtcta aaacacccac
     3181 ccaccaccga attgccatga aataccagcc cgatatcatc agtgtgcaat cgatccacag
     3241 ttacggcccg ggctggattc aggtcggtgc caccaaactg acccacagcg tgatccttgg
     3301 ctcgcgtggc gaacggctgg attggcattg cgaacgcttt gaagacctga cacccgccca
     3361 ctttgaacag ttggcacaaa tgcaaaccga gctggtgatt ttcggcagcg ggccgcgttt
     3421 gcgttttgtc gcacccaggc tatcgtgctc gctgatcgaa cggcaaattg gcatggaaac
     3481 catggacacc cacgcagctt gccgaactta caacattttg gcaagcgaag gccgttgcat
     3541 agctgtggcc ttgctgatgc agtaaaatcc cgggcttgca gtcgagggcg caggcgctta
     3601 cccaggatag cgtccacgac ttgagcgatc gacgtatccc gtcacacgag attgaagaaa
     3661 catggcgatt gttgtcaata aacccattcc tgaattcgag tctaatgcca caggaggcgt
     3721 tagagtcacc aacacttctc acttgggccg tatcgtcgtt ttgtattttt accccaagga
     3781 caacacgccg ggttgtacca ccgaagcgat ggagtttcgc gacaagtaca aagaatttgt
     3841 gaaagccggt gcaacggttt ttggcgtttc ccgcgacaac atgcgctcac acgacgagtt
     3901 caagcaaaag cttgagttgc cgttcgagct gatcgccgac accgaagaaa aaatgtgtca
     3961 tatgtttggc gtggtaaaaa acaagatcat gtacggcaag aaggtcaagg gcattgagcg
     4021 cagcaccttc ctgattggtg ccgatggctg cctcaaagat gaatggcgcg gcttgaaagt
     4081 ccctggtcat gtcgatgagg tactcgagtc tgtcaaggaa ctcaagaagt cagcagtctt
     4141 ggcctgaccc cgacctcggt catggggtcc aactttcatg cactctgtat ccccccccgg
     4201 acatgactgc tatttcacgt taaccagcag caaggacatg gtcttcaatc acttcgccat
     4261 ttggcctgtc gggctcgggc gcactgcgcc gttgcatatc gccagtgtgg ctcgttgcgt
     4321 tttgcgtctg gcattgcaac ccaaatcctt cggccaaacg cgttgcttat tttcgaccct
     4381 ctcctgagtg gtgtggcggc gcagtctgcc cgagcgcctt tgaggctcac gcacaatctt
     4441 tgcaccttgt tgacgactca ggttcttgtt tcgaaacagc cgccctggtg acatggcggc
     4501 tgtttttttt ctgaccagcg atttttcacc ccatttctgg gagctctcat gccactgcct
     4561 tcaccgccga gcaaacgcgc cgccttgttg tctctgaaag accttgatgc accgctggcg
     4621 aaatccaaaa ccagggccga ccgggtccaa acacccgctg ccgcgccggc cacacccgct
     4681 gccctctcgg cagcgacccc gcctttgatt gccacggtca cattgccttt atcgcagagc
     4741 gctacggctg agcagatgac ggtggcacca caacagtctg tggcaaaggc actgccgcga
     4801 gtcaaatcca aatcccgtca aacccggggc ccgggcaagc tgtttgtcct tgacactaat
     4861 gtgttgctgc atgacccgac ctgcctgttc cggtttgaag agcatgacat ttttctgccg
     4921 atgatcgtgc tcgaagagct cgatgcgcac aagaaaggca tgaccgaagt tgcccgcaac
     4981 ggccggcaaa ccagtcgctc actggacgcg ctggcggaac atccggtggc aatgtcggcc
     5041 ccctgatgtt ccgccagcgc gtccagcgag cgactggt
//
LOCUS       DAOSLL010000328         5078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14703_length_5078_cov_2.859845, whole genome shotgun sequence.
ACCESSION   DAOSLL010000328 DAOSLL010000000
VERSION     DAOSLL010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5078
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14703_length_5078_cov_2.859845"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="PLB25_13830"
     CDS             <1..826
                     /locus_tag="PLB25_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013357546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="D-ribose ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE42692.1"
                     /translation="PFFKAEALGAQARAKELGYDTISLVHDDNANKQNELFDTAIARK
                     AKAIILDNAGSEATVAAVKKAKDAGIPSFLIDREINATGIAISQIVSNNYQGAQLGAE
                     EFVKLMKEKGKYVELVGRESDTNAGVRSKGYHDVIDKYPDMKMVARQSANWSQPEAFK
                     LMESILQANSDIKGVISGNDTMAMGAWAALVAAKRTDVIVVGFDGSNDVRDSIKKGGI
                     KATVLQPAYRQAQLAVEQADKFLKTGSTGQPEKQLMDCILINSKNAAKLETFTLAK"
     gene            916..1596
                     /locus_tag="PLB25_13835"
     CDS             916..1596
                     /locus_tag="PLB25_13835"
                     /inference="COORDINATES: protein motif:HMM:NF021565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2291 domain-containing protein"
                     /protein_id="HOE42693.1"
                     /translation="MGTPKNNRVIQQAVVLCAVCLMLTGCKIERKSEQDKLKQVAAAG
                     SNATSSFDAAAEVRGMWEPKVLPAINQLAVDFRELKKAMDANLDAAGVKHGHREKGEG
                     APWNLAARISGKIVDADTEVRAGTADIDVDGDGRADVQLQMGPIVKGTTLRDILPFVS
                     FTSYTNQIDFAQLANALNDRAYESALKSVDRANLKGKSVEVLGVFTTDNASDVPLVTV
                     TAFKVLGK"
     gene            1671..2591
                     /locus_tag="PLB25_13840"
     CDS             1671..2591
                     /locus_tag="PLB25_13840"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOE42694.1"
                     /translation="MALKAVDFELYAGAVNVLVGENGAGKSTMMKVIAGVESPSEGQI
                     WLDGKAVQLGSTEVARQHGIGIVFQELNLFPNLTIAENMFIGREISSRMYAIDSEAQA
                     KKAEQLLGELEHHLDPNLLVGELRIGEQQIIEIAKAIGQNARILILDEPTSALSAPEV
                     EILFRVINDLKSRGVAIVYISHRLEELIRIGDYITVLRDGCVTGSAKMKDVDLTWIVR
                     QMVGSNTKNFARAGDNRLGETVLEVNHVCLPRLGAPGYLVDDMSLSLRAGEIVGIYGL
                     MGAGRTELFECIYGVTLTARRAASNCLARN"
     gene            2603..3139
                     /locus_tag="PLB25_13845"
     CDS             2603..3139
                     /locus_tag="PLB25_13845"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE42695.1"
                     /translation="MTSCIDAGLALIPEDRKTDGLVHSMSILENLTLASLWRLVTLGY
                     LSPEVESSAAGKFVKSLSIKTNNPSLEIGSLSGGNQQKVVIGRALMTDPKVLLMDEPS
                     RGIDIGAKAEVFKVMRDLAKTGLGILFVTSDLEEVMALSDRIVVMSHGQLAAQFGPNE
                     ATQDAVVLASNSQVLLAA"
     gene            complement(3161..3337)
                     /locus_tag="PLB25_13850"
     CDS             complement(3161..3337)
                     /locus_tag="PLB25_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42696.1"
                     /translation="MISALAALKKFGAMTAKKPATRNSAMKVRRRKTKSRALSPVVVD
                     AERSEETAEVDMMD"
     gene            3336..4241
                     /locus_tag="PLB25_13855"
     CDS             3336..4241
                     /locus_tag="PLB25_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE42697.1"
                     /translation="MSKHVALNAFLAIGMTYVIVSGGIDLSVGSTVGLTGMVAGGLIL
                     HGLTIGDYVIYFNVLEIVGIALMVGLIVGAINGFLITQLNVAPFIATLGTLYVARGIA
                     LLSSDGSTFPNLEGSAEHGTTGFPLIGTLSLAGVPLVIWLLVVMGLGAAYLGARTPLG
                     RHIYAVGGNERAAMLSGVRVNRIKFFVYMFSGFCASIVGLIISSQLVASHPMTGETFE
                     LNAIAAAVLGGTSMTGGRGKIGGTIIGAFVIGILSDGLVMMGVSAFWQTVIKGLVIIA
                     AVVVDQFQQRMQQRMAYQMRAEAQK"
     gene            4338..>5078
                     /locus_tag="PLB25_13860"
     CDS             4338..>5078
                     /locus_tag="PLB25_13860"
                     /inference="COORDINATES: protein motif:HMM:NF016114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding domain-containing protein"
                     /protein_id="HOE42698.1"
                     /translation="MPSPRFASHDLLRLTTRVASMYHERAMGQVQIAEQLGLSQARVS
                     RLLKQAPGLGIVKTTVHIPEGVHADIEQQLEQRYGLDEVIVVEATEAAQTDDELMTRA
                     LASSVASYLELIVPTLSVVGVSSWSSTLLAAVNAMRPMSPGETKSIVQVLGGVGLPKA
                     QHSATRLTERLAQLAKAEPVFMLAPGVVATPAAKSALQADPLCHQAMARFGDLSALLM
                     GIGSIPPSRMLRESGNVFTEQDLTELRDA"
BASE COUNT         1080 a         1429 c         1494 g         1075 t
ORIGIN      
        1 cccgttcttc aaggccgagg ccctgggcgc acaagcgcgg gccaaggaac ttggctacga
       61 cacgatttcg ctggtgcatg acgacaacgc caacaagcaa aacgaactgt tcgacaccgc
      121 gattgcccgc aaagccaaag ccatcattct ggacaacgct ggctccgaag ccacagtcgc
      181 tgcagtgaaa aaggccaagg acgcaggtat tccctcgttc ctgatcgacc gcgaaatcaa
      241 tgcgactggc atcgccatct cgcagatcgt ctcgaacaac taccagggtg cccaactggg
      301 tgcggaggag tttgtcaagc tgatgaaaga aaaaggcaaa tatgttgagc tggttggccg
      361 tgaatccgac accaatgctg gcgtgcgctc caagggttac cacgatgtga tcgacaaata
      421 ccccgacatg aaaatggtgg ctcgtcaatc cgccaattgg agtcagccag aagccttcaa
      481 gctgatggaa agtattttgc aggccaactc tgacatcaag ggcgtgatct ctggcaatga
      541 caccatggca atgggcgcgt gggcagccct ggtggctgcc aaacgcaccg acgtgatcgt
      601 ggttggcttt gacggcagca atgatgtgcg cgactcaatc aaaaagggcg gcatcaaggc
      661 cacggtcttg caaccggcgt accgtcaggc ccagttggca gtcgagcagg ccgacaagtt
      721 cctcaagacc ggcagtactg gacagccaga aaagcagttg atggattgca tcttgatcaa
      781 tagcaaaaat gcggccaagc tggaaacctt cactttggcc aagtaagtcc cctcaggtcg
      841 ttgagcgttt gttctcgacg acctgaattt acgcgctgac cgctcggcat gagtggcaac
      901 ctcaggaata agtccatggg cacgcccaaa aataacagag tgattcaaca agcagtggtt
      961 ctgtgtgccg tctgtctgat gctgactggc tgcaagatcg agcgcaagtc cgagcaagac
     1021 aaactcaagc aagttgcagc ggcaggcagc aacgcgacct cgtcctttga tgcagcggca
     1081 gaggttcgtg gcatgtggga gcccaaggtt ctgccagcca tcaaccagtt ggccgtcgat
     1141 ttccgtgagc tgaagaaagc catggacgcc aatcttgacg cggctggcgt taaacacggt
     1201 caccgtgaaa aaggcgaggg cgcaccctgg aatctggcgg ccagaatcag cggaaaaatt
     1261 gtcgacgccg ataccgaagt cagggctggc acagccgaca tcgatgtcga tggcgacggt
     1321 cgggccgacg tgcaattgca gatgggcccc atcgtcaaag gcacgacttt gcgcgacatc
     1381 ctgccgtttg tgtcctttac ttcatacacc aaccagatcg atttcgctca actggcgaat
     1441 gccctcaatg accgcgccta tgaatcagcc ctgaaatcgg ttgatcgtgc caacctcaag
     1501 ggcaagagcg tggaggtgct tggtgtgttt accacagaca acgcctccga tgtgccattg
     1561 gtcaccgtga ccgcattcaa ggtgcttgga aaatgaaacc gctgacatcc ccgcagtcgc
     1621 accccgtggt cctgaagctc gaaggcgtga gcaaggtcta ccccggcacc atggccctca
     1681 aggcggtaga tttcgagctt tacgccggcg cggtgaatgt gctcgtgggt gagaacggcg
     1741 ccggaaaatc caccatgatg aaagtcatcg ccggtgtgga gtcaccaagt gaaggtcaga
     1801 tctggctcga cggcaaggcg gtacaactcg gtagcaccga ggtggctcgc cagcacggta
     1861 tcggcattgt gttccaggaa ctgaatctct ttcccaatct gaccatcgca gagaacatgt
     1921 tcatcgggcg tgaaatcagc tccaggatgt atgcgatcga cagcgaggcg caggccaaaa
     1981 aagcagagca actgctgggc gaactggagc accatcttga tcccaatctg ctggtcggcg
     2041 aactccgtat cggcgaacag cagatcattg aaattgccaa agccatcggg caaaacgccc
     2101 gcattctgat cctcgacgag ccgacttcgg cgctaagtgc ccccgaagtt gaaattctct
     2161 tccgcgtcat caacgacctc aagtcgcgtg gtgtggccat cgtctacatt tcccaccgtc
     2221 tcgaagaact gattcgaatc ggtgattaca tcaccgtgtt gcgcgatggt tgtgtcactg
     2281 gttcggccaa gatgaaagac gttgatctga cgtggatcgt caggcaaatg gttggctcca
     2341 acaccaagaa tttcgcccgt gcgggtgaca atcggctcgg ggaaaccgtg ctcgaggtca
     2401 atcacgtttg cttgcctcgg ctgggcgcgc cgggctacct ggtcgacgac atgagcctgt
     2461 cgctgagagc cggtgaaatc gtcggaattt atggcttgat gggtgctggc cgaaccgagt
     2521 tgtttgaatg catttacggt gtcaccctga ccgcgcgacg ggcagcgtca aactgcttgg
     2581 caaggaactg atcggcagta acgtgacctc ctgcattgat gccggactgg cgttgattcc
     2641 ggaagaccgc aagacggacg ggttggtgca ctcgatgtcg attctggaaa acctcacttt
     2701 ggcgagcctg tggcggctgg tgacgctggg ctatctgagc cccgaggtgg aaagctcggc
     2761 ggcaggcaag ttcgtcaagt cgctgtcgat caagacgaac aaccccagcc tggaaattgg
     2821 ttccttgtct ggcggaaacc aacagaaagt ggtcatcggc cgggccttga tgactgaccc
     2881 caaggtgctg ctgatggatg agccaagccg tggcattgac attggcgcca aggctgaggt
     2941 tttcaaagtc atgcgtgacc ttgccaaaac cggccttggg atcctgtttg tgacctcgga
     3001 tctcgaagaa gtcatggcgc tttcggaccg catcgtggtg atgtcccacg gccaattggc
     3061 ggctcagttc ggacccaatg aggctaccca agacgcggtg gtcctggctt caaacagtca
     3121 ggtgctgctg gcggcatagg acttccaccc cttctttaat tcaatccatc atgtccactt
     3181 cagccgtttc ctcacttcgc tccgcgtcca ccacgacggg cgacaacgcc ctgcttttgg
     3241 tcttgcgcct gcgcactttc attgcactgt ttcttgtggc aggttttttc gcagtcatgg
     3301 cgccgaattt cttgagcgca gccaatgcgc tgatcatgtc aaagcatgtt gcgctcaacg
     3361 ccttcctggc cattggaatg acctatgtca tcgtcagtgg tggcatcgac ctgtcggtgg
     3421 gttcgacggt tgggctgacc ggcatggtcg ccggtggcct gatccttcat ggcttgacga
     3481 ttggtgacta cgtgatctat ttcaatgtgc ttgaaatcgt cggtatcgca ctgatggtcg
     3541 ggttgattgt tggtgcgatc aacggctttc tgatcacgca gctgaatgtt gcgcctttca
     3601 ttgccacgct gggcacgctg tacgtggccc gcggcattgc gctgctgtcg tcggatggct
     3661 cgacctttcc caacctcgaa ggcagtgccg aacatggcac aaccggtttc cccttgatcg
     3721 gcaccttgag tctggcgggt gtacccctgg tcatctggct gctggtggtg atgggtctgg
     3781 gagccgccta tctgggcgcc cgcacgccgt tggggcgcca catttacgcc gttggcggca
     3841 atgagcgggc ggccatgctc tccggggtga gggtcaacag gatcaaattt tttgtctaca
     3901 tgttttcggg tttctgtgcc tccattgtcg gcctgatcat ctcttcgcag ctggtggcct
     3961 cgcaccccat gaccggcgag accttcgaac tcaacgccat cgccgcagcc gttcttggcg
     4021 gcacgtccat gaccggtggt cgcggcaaga ttggcggcac catcatcggc gcatttgtca
     4081 tcggtatcct gtcagatggc ctggtcatga tgggtgtcag tgcattctgg cagaccgtga
     4141 tcaaaggact ggtcatcatc gccgccgtgg tggtggatca gtttcaacag cggatgcagc
     4201 agcgcatggc ctaccagatg cgcgcagagg cccaaaaata agcatcagga gtggcgcggc
     4261 gcacttcttg ccaatgcctt tgccaaggat gctattgagc gagaatactc ggctagtccc
     4321 agaaaaccaa caccgacatg ccttcaccgc gctttgcatc ccatgacctg ctgcgcctca
     4381 cgacgcgggt ggcgagcatg tatcacgaac gcgccatggg gcaggtgcag atcgccgaac
     4441 agctcgggct gtcgcaggcc cgggtatcgc gtttactcaa gcaggctccg ggccttggaa
     4501 tcgttaaaac cacagtgcac attcctgaag gggtgcacgc cgacatcgaa cagcagcttg
     4561 agcagcgcta tggcctcgac gaggttatcg tcgtcgaggc cacagaggcc gcgcagaccg
     4621 atgacgaact gatgacacgg gcgctggcct ccagcgtggc cagctacctt gaactgatcg
     4681 tgccaaccct gtccgtggtt ggggtgtcct cttggagttc aaccctgctg gcagcggtca
     4741 atgccatgcg tccgatgagc ccgggcgaga ccaaaagcat cgtccaggtg ctcggcggcg
     4801 tgggtttgcc gaaggcccag cattcggcca cccggttgac tgaaaggctg gcccagcttg
     4861 ccaaggctga accggtgttc atgctggcgc caggggtcgt tgccacgccg gcggccaaga
     4921 gtgccctgca agcggacccc ttgtgccatc aggccatggc gcgtttcggc gacttgtctg
     4981 ccttgttgat gggtattgga tcgattccgc cttcacgcat gttgcgcgaa agtggcaacg
     5041 tgttcaccga acaggacctg accgagctgc gcgatgcc
//
LOCUS       DAOSLL010000329         5077 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14705_length_5077_cov_5.779172, whole genome shotgun sequence.
ACCESSION   DAOSLL010000329 DAOSLL010000000
VERSION     DAOSLL010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5077)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5077)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5077
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14705_length_5077_cov_5.779172"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="PLB25_13865"
     CDS             <1..460
                     /locus_tag="PLB25_13865"
                     /inference="COORDINATES: protein motif:HMM:NF018032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HOE42699.1"
                     /translation="STVWNKYQGIALRNLPVLHPHLRWREFRPGALNKAWQQAVKGHD
                     LDAVGDLYALALPDDIPVTCQTVWQAALELQSFARAHPGSTHPLVAVTSQDVGKALGM
                     ELFRLVPGRELLIIDEVHTRAGDYLDIGKSYFSGGTIPITVKSLAFPQAA"
     gene            883..2244
                     /locus_tag="PLB25_13870"
     CDS             883..2244
                     /locus_tag="PLB25_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009664652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HOE42700.1"
                     /translation="MMLSTRLFGHGYTFKGVKDVLAKANEARSGDRLAGIAAQDARER
                     VAAKQVLAELLLSDLRNNPVVPYEDDCVTRLIQDNVNEAVYQRIQHWSVSELREYLLG
                     DTASPEELEVLRKGLTSEMVAAVAKICSNGDLIYGAKLLPVVTKANTTMGLPGRFSSR
                     LQPNDTRDDIRSISAQIYEGLSYGSGDALIGVNPVTDNVESVTRILHQVDEVIEKHQI
                     PTQGCVLAHISTQMEAVRKGAPIGLLFQSICGSEKGLKEFGITLAMLDEAADVGRHYS
                     RVAGGNCLYFETGQGSALSANAHFGADQVTMEARNYGLARHYSPFIVNTVVGFIGPEY
                     LYNSREILRAALEDHFMGKLSGISMGCDCCYTNHADADQNSNESLVMLLASAGCNFVI
                     AMPMGDDIMLNYQTCSFHDIATVRQTLGLRPAPEFEHWLEARGLMSNGKLTRSAGDPS
                     LFF"
     gene            2257..3135
                     /gene="eutC"
                     /locus_tag="PLB25_13875"
     CDS             2257..3135
                     /gene="eutC"
                     /locus_tag="PLB25_13875"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003971.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HOE42701.1"
                     /translation="MNEAQIADIVRAVMRELEQAPMPSSPAETPVAGELPDLGSDEAR
                     TWIGVTDTKSADVLTELRRSTRARVCAGRTGPRPRTQSLLRFLADHSRSKETVLSEIP
                     FEWVQHAGLLEVQTEINEKGQYLTRPDLGRVLSAKGLEIVKTQCIKAPDVQLLISDGL
                     STEATTANFDDIVPPLIRGLESLQMKAGTPLFVRFGRVKVEDQIGELLQAKVVILLIG
                     ERPGLGQSESLSCYMVYQPTSQTVEAERTCISNIHRGGTPPIEAAAVIAELAERMLKE
                     QCSGIALGKKMAGGNA"
     gene            3937..4593
                     /gene="eutL"
                     /locus_tag="PLB25_13880"
     CDS             3937..4593
                     /gene="eutL"
                     /locus_tag="PLB25_13880"
                     /inference="COORDINATES: protein motif:HMM:NF011934.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HOE42702.1"
                     /translation="MSTFEKIPVSVCAVRLISSLQADFKAELKLDDSIFSLGVFTADS
                     DDVAYIAADEATKQANVRVVFGSSLYAGARHGPSSTAGEVILMLAGPSPAEVRSGLDA
                     ALNAVRDGAAFQWANDAHTVAFLAHVVTRCGSYLAEVAGVREGDSLAYLVAPPLEAAV
                     GLDAALKAAEVRLAAYVRPPSPTNYSGAYLQGSQAACRAACDAFARTVADIAAQPLVY
                     "
     gene            4595..4885
                     /locus_tag="PLB25_13885"
     CDS             4595..4885
                     /locus_tag="PLB25_13885"
                     /inference="COORDINATES: protein motif:HMM:NF013129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HOE42703.1"
                     /translation="MNNAYGFLEVRGLLAGIVCADAMLKSASVRLASQITTRPGLITL
                     VVEGDIGSCRAAIDVGRQTAEHLGGLVSEKVIGRPEPDLDLFLPSVSNRRPL"
     gene            4909..5016
                     /locus_tag="PLB25_13890"
                     /pseudo
     CDS             4909..5016
                     /locus_tag="PLB25_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_176968269.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1013 a         1602 c         1540 g          922 t
ORIGIN      
        1 cagcacggtc tggaacaagt accagggcat tgcgctgcgc aatctgccgg tgctgcaccc
       61 gcacctgcgc tggcgcgagt ttcgacccgg tgcgctcaac aaggcctggc aacaggccgt
      121 caaaggccat gaccttgacg cggtcggtga cctgtacgcg ctggcgctgc cggacgacat
      181 tccggtcacc tgccagacgg tctggcaggc cgcacttgag ttgcaaagtt tcgcccgggc
      241 acaccccggc agtacccacc cgctggtggc ggtgacttcg caggatgtcg gcaaggcgct
      301 tggcatggag ttgtttcgcc tggtgcctgg gcgcgaactg ctgatcatcg atgaggtcca
      361 cacccgcgct ggcgactacc tggacattgg caagagctac ttcagcggcg gcacgattcc
      421 gatcaccgtc aagtcgctcg cctttcccca ggcggcatga ccccagatgc ctcagttgac
      481 tgattgtttt catctaccag agcttatgag catttactta ttttgtttca tcggggttgc
      541 gccggcgggt cacagcgcga cgcagccgtt tgaaccgcac ctgggggcct ttttcgcccg
      601 ccagcaccac agacgctcgg tggcgcgcgt tgtcgagcgt cgcagcttgc tgcggcaggc
      661 agggcgcggc cataccacca ggctggtttg cagcccgacg cggccgcgtc ggcccgctcg
      721 ggcgcggcca cctgagcaag ccagcttgaa cttcatcgcg ccagccattt tctgcaaagc
      781 tcgccacttc ccggcgcgac ctgtcgctgc catggactac ttcgcgcatt cgcaggccag
      841 aacccattgc caatcagttt cgctattgaa ggacaaccca acatgatgct ctcaacccgt
      901 ttgtttggac acggctacac cttcaagggg gtcaaggacg tgctggccaa ggccaacgag
      961 gcccgctctg gcgaccggct ggctggcatt gccgcgcagg acgcgcgtga acgcgtggcg
     1021 gccaagcagg tgctggcaga actgctgctg tctgacttgc gcaacaaccc cgtggtgccg
     1081 tatgaggacg actgcgtgac gcggctgatt caggacaacg tgaatgaagc ggtttaccag
     1141 cgcattcagc actggagcgt gagcgagctg cgcgaatacc tgctgggcga cacggcctcg
     1201 cccgaggagc tggaagtgtt gcgcaagggg ctcacttcgg aaatggtggc ggccgtggcc
     1261 aaaatctgct cgaacggcga cttgatctac ggggccaagc tgctgccggt ggtgaccaag
     1321 gccaacacca ccatgggtct gcccggtcgt ttcagcagcc gactgcagcc caacgacacg
     1381 cgcgacgaca ttcgcagcat ctcggcacaa atttacgagg gcctgtcgta tggctcgggc
     1441 gacgcgctga tcggggtcaa cccggtcacc gacaacgtcg aatcggtcac ccgcatcctg
     1501 catcaggttg atgaggtgat cgagaagcac cagattccaa cccagggctg cgtgttggcg
     1561 cacatcagca cgcaaatgga agcggtgcgc aagggcgcgc cgattggcct gctgttccag
     1621 agcatttgcg gcagcgaaaa aggcttgaaa gaatttggca tcacgcttgc catgctcgac
     1681 gaggcagccg atgtcgggcg gcattattca cgcgtggccg gtggcaactg cctgtatttc
     1741 gagaccggcc agggctcggc gttgtcggcc aacgcccact ttggtgccga ccaggtcacc
     1801 atggaagcgc gcaactacgg tctggcgcgg cactactcgc ccttcatcgt caacaccgtg
     1861 gtcggcttta tcggccccga gtacctttac aacagccgcg aaatcctgcg cgccgcgctg
     1921 gaagaccact tcatgggcaa gctatcgggc atttcgatgg gctgcgactg ctgttacacc
     1981 aaccatgccg atgccgacca gaattcaaac gaaagcctgg tgatgctgct ggccagcgcc
     2041 ggctgcaact ttgtcattgc catgccgatg ggcgacgaca tcatgctcaa ttaccagacc
     2101 tgttcgttcc atgacatcgc cacggtgcgc cagacgctag gcctgcggcc agcgccggaa
     2161 tttgaacact ggctggaagc gcgtggcctg atgtcaaacg gcaagctgac ccgctccgcc
     2221 ggtgacccct cgctgttttt ctgatcggag cgcaccatga acgaagccca gattgccgac
     2281 attgtccgtg ccgtgatgcg cgaactggaa caagccccca tgccatcaag ccctgccgag
     2341 accccggtgg caggggaatt gcccgacctg ggcagcgacg aagcgcgcac ctggatcggc
     2401 gtgacggaca ccaaaagcgc cgatgtgttg accgaactgc gccgcagcac ccgcgcccgc
     2461 gtctgcgccg gacgcactgg cccgcgcccg cgcacccagt cgctgctgcg ctttctggcc
     2521 gaccattcgc gttccaaaga aaccgtgctg agcgaaatcc cgtttgagtg ggtgcaacac
     2581 gcaggtttgc tggaggtgca gaccgaaatc aatgaaaaag gccagtacct gacccgcccc
     2641 gacctgggcc gggtgctcag cgccaagggg ctggagatcg tcaaaaccca gtgcatcaag
     2701 gcccccgacg ttcagttgct gatctcggac ggcctatcga ccgaagccac caccgccaat
     2761 tttgacgaca tcgtgccgcc actgattcgc gggctggagt cgctgcagat gaaagctggc
     2821 acgccgctgt ttgtgcgctt tggtcgcgtc aaggtggaag accagattgg tgagctgctg
     2881 caggccaagg tggtgatcct gctgattggc gagcgccccg gcctggggca gtccgagagc
     2941 ctgtcgtgtt acatggtcta tcagcccacc agccagacgg tggaagctga acgcacctgc
     3001 atttccaaca tccaccgggg cggcacaccg ccgattgaag ccgccgccgt gattgccgaa
     3061 ctggctgagc gcatgctcaa agagcagtgc agcggcatcg ctttaggcaa aaaaatggcg
     3121 ggcggcaacg cctgatggct tggaaccggc ccccgacgat gagcatcatc caattgccgt
     3181 cagtcgcagt ctgcctgccc aaccgcgcca atagcaagcg gcaatggaca agccagacct
     3241 gcctgcccaa cagcgcgaat gccacgaacg cagcagcaga tttggggtcg gatcccaatt
     3301 cggcccggca gttttcaatt cgtcacgaac ccatccccga attgggctca gaccccaatt
     3361 ctgcgggcca tgccgagacc tcgcatcagg caggcatcag caagcgcagt gacaaagtga
     3421 ccatcaaagt gctgccacaa aggcgaagca ggagcatccg gcgacgcggg caaatgggcg
     3481 agcggtgggg cgcgggaaag ttatttacta ttactttagt agcttctagc gcatacccta
     3541 taagcgctag aggcgaaaat cactatcaag ccaaaccgca ggaacacccc gcagcaatgg
     3601 cccgcagaat tggggtcaga gcccaattcg cagatgcacc acccgcagca atgagcgcac
     3661 ctgagcgaat tgggatccga ccccaaatct gctaccgcat gccagaactt ggcgcagagg
     3721 tcagagccca attcggagtt acaccacccg cagctttgag cgcacctggc cgaattggga
     3781 tccgacccca aatctgctat cgcgtagctg aactggtcga tatcaattca atagctgtca
     3841 gcgcttatcc cataagggat ggtggcataa gtcaccatca agctatggtg gaatgccgcg
     3901 cacaacccgc cccttgactc aaccctgaaa tcaaccatgt ccacctttga aaaaatcccg
     3961 gtatcggttt gtgcagtgcg gctgatctcg tcgctgcagg cagatttcaa ggccgaactc
     4021 aagctcgatg acagcatttt cagcctcggg gtgttcactg ccgattcgga cgatgtggcc
     4081 tacatcgcag ccgacgaagc gaccaagcag gccaatgtgc gggtggtgtt cggcagctcg
     4141 ttgtatgccg gggccagaca cggaccatcg agcacggcgg gcgaagtgat cctgatgctg
     4201 gccgggccct cccccgctga agtgcgctca gggctggatg ccgcgctcaa cgcagtgcgc
     4261 gacggcgctg cctttcagtg ggccaacgac gcgcacaccg tcgcctttct ggcccatgtg
     4321 gtgacccgct gcgggtccta tctggcagag gtggcgggtg tccgcgaagg cgattcgctg
     4381 gcttacctgg tggcgccacc gctcgaagcc gcagtcggcc tggatgccgc cctgaaggcg
     4441 gccgaggtgc ggctggcagc ctatgtgcgc ccaccgtcgc cgaccaatta ttcaggtgcc
     4501 tacctgcaag gctcgcaggc cgcctgtcgc gccgcctgcg atgcgtttgc ccgcaccgtg
     4561 gccgacattg cggcgcagcc gctggtgtac tgacatgaac aacgcctatg gttttctgga
     4621 ggtccggggc ttgctggccg gcatcgtttg cgctgatgcc atgctcaagt ctgccagcgt
     4681 acgcctggcc agccagatca ccacccgccc tggcctgatc acgctggtgg tggaaggcga
     4741 cattggttcc tgccgtgccg ccattgatgt cggtcggcaa accgctgagc acctgggcgg
     4801 cctggtcagc gaaaaagtga ttggtcggcc agagcccgac ctggacctgt ttttaccgtc
     4861 cgtcagcaac aggagaccgc tgtgaacgaa atcgcccgca aggcccgcga agccggtgtg
     4921 gtcggctctg gcggtgccgg atttccgacc cacgtcaagc tgctgagccc agccgagatc
     4981 tacctggtca acggggccga atgtgaaccg ctgctcaagg tggaccagca actggccgtc
     5041 aaatacgcgc cgcaactggt gcgcgggctg caactgg
//
LOCUS       DAOSLL010000330         5076 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14712_length_5076_cov_3.811790, whole genome shotgun sequence.
ACCESSION   DAOSLL010000330 DAOSLL010000000
VERSION     DAOSLL010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14712_length_5076_cov_3.811790"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="PLB25_13895"
     CDS             complement(<1..984)
                     /locus_tag="PLB25_13895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE42704.1"
                     /translation="MTSTAHSLPSSVSFKQDATIIGLVGLAHAASHFGHLLLPLMFPV
                     FMAEFGLSYSQVGLLMSTFFVVSGLGQASAGFVVDKVGARPMLFLALSLFALACAGAS
                     LVTGYYALFAVAALAGLGNATFHPVDFTILNQRVSPARLGHAFSAHGLTGNLGWAAAP
                     VFLVGISALYSWRTAYVAACLMYLGILLLMVLQRDKLLTHAAVHHPDRPAEHSMAFMK
                     LPVVWWCFAFFMLSSMTLAVVQSFAVSILKAMHGISFETATLTITAYMLCGAFGMFIG
                     GFVAATSRHSDRVVALSMSGAAVLLALCGTGLFGATVTMVVLAATGFAVGIG"
     gene            complement(1035..2135)
                     /locus_tag="PLB25_13900"
     CDS             complement(1035..2135)
                     /locus_tag="PLB25_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42705.1"
                     /translation="MKIAQSTIAMQSQHASVSLRTSHTTMRAWVGNQRPDFEGQRPVQ
                     QDAAAVRLTLSQAASKRLQSQLPATSNAATAKADAVAQADAAVRNDPRLAMLIDMVES
                     ITGRRIRVFDARELQSGASNADLPVQLGASGSQAAAAAQPAAPAGWGLELDSRQTIAE
                     SETTQVSAQGVVQTADGQQISFTLQLEMSRSFVQESSISIRSGDAVRKDPLVINFGGA
                     GVALTDTQFAFDLNADGQQENIAFVAGGSGFLALDKNGDGKINDGSELFGPRSGDGFA
                     DLAKYDQDGNQWIDDNDAVFSQLRVWQRDVSGQDHLTSLAQSGVGALYLGRVASPFSV
                     NTASNQSLGLVRSTGVFLYESGQAGSLQQVDL"
     gene            complement(2239..3150)
                     /locus_tag="PLB25_13905"
     CDS             complement(2239..3150)
                     /locus_tag="PLB25_13905"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007865768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HOE42706.1"
                     /translation="MLNKDQTQDFTALMPGQTSEAGASRRTALKVALGVGYAAAAAPI
                     MAQTAIKTSSEGLRTGESVYDMTGFSVPFFFAMPEGKKNLPVVLVIQEIFGVHEHIAD
                     ICRRFAHVGYLAIAPELFARQGDPASYTQVAKLMAELVSKVPDAQVMGDLDGAVKWAG
                     ENGGDLKKVAITGFCWGGRITWLYAEQSKNVKAAVAWYGRLVGTASDLTPKHPLDLAA
                     GLKAPVLGLYGGQDSGIPLVTINQMKDALAEAGAKGNAAAKASQFVVYKDAPHAFHSD
                     YRPSYRKDDAADAFQRALAWFKAHGVA"
     gene            3379..4620
                     /locus_tag="PLB25_13910"
     CDS             3379..4620
                     /locus_tag="PLB25_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase
                     (2-methylpropanoyl-transferring) subunit alpha"
                     /protein_id="HOE42707.1"
                     /translation="MPQRQTLHLHVPEPTGRPGRDTDFSYLPLSAAGAKRRPPVDVAA
                     AQTNDLAFALIRVLDDSGQALGPWAPQMDSALLRRGLRAMLLTRAFDARMLIAQRQKK
                     MSFYMQCLGEEAIACAHALALSEGDMCFPTYRQQGLLLTREDNNMVDMICQLYSNEHD
                     PMKGRQLPVMYSSKKQGFFSISGNLATQVIQAVGWAMASAIKGDSKVASSWIGDGATA
                     EADFHTGLTFAHVYRAPVILNVVNNQWAISTFQAIAGGEGTTFAARGVGNGIASLRVD
                     GNDFLAVYAASRWALERARSNFGPTLIEWVTYRAGPHSTSDDPSKYRPANDVARFPLG
                     DPIARLKQHLIVLGIWSDAEHEAVQKEVEAEVAAAQKEAEKYGTLADGPTQSLSGMFE
                     DVYKDMPVHLQRQRAELEGQS"
     gene            4617..>5076
                     /locus_tag="PLB25_13915"
     CDS             4617..>5076
                     /locus_tag="PLB25_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HOE42708.1"
                     /translation="MSDMPNTSPMTMIQALRSAMDVMLERDNNVVVYGQDVGYFGGVF
                     RCTEGLQKKYGNQRVFDAPISEGGIVGTAVGMGAYGLRPVVEIQFADYVYPATDQIVS
                     EAARLRNRSAGDFTCPITIRMPCGGGIYGGQTHSQSPEAMFTQVCGLRTVM"
BASE COUNT         1105 a         1575 c         1443 g          953 t
ORIGIN      
        1 gccaatgccc acagcaaagc cggtagcagc cagcaccacc atggtcaccg tcgccccaaa
       61 aaggccggtg ccgcacagcg ccagcaacac cgccgcaccc gacattgaga gcgccaccac
      121 acggtcactg tggcgggagg tcgccgccac aaatccgcca ataaacatgc caaaggcacc
      181 gcacagcatg taagcggtaa tggtcagggt ggcggtctca aaactgatgc cgtgcatggc
      241 tttcaggatc gacacggcaa aactctgcac caccgccagc gtcatggaag agagcataaa
      301 gaaggcaaaa caccaccaca ccactggcag cttcatgaac gccatgctgt gctcggccgg
      361 gcggtccggg tggtgcacgg cagcatgggt cagtagtttg tcgcgctgca acaccatcag
      421 caacaaaatg cccaggtaca tcaggcatgc agccacatag gcggtgcgcc aactgtacag
      481 ggcactgatg ccgaccaaaa acactggtgc tgcggcccac cccagattgc ctgtcaagcc
      541 atgcgcactg aaagcgtggc ccaggcgcgc aggcgacacg cgctggttca ggatggtgaa
      601 gtccaccggg tgaaaggttg cgttgcccaa gccggccagc gccgccacgg caaacagtgc
      661 gtagtagccg gtcaccagcg aggcaccagc acaggccagg gcaaacaggc tcaaggccag
      721 aaacagcatg ggccgcgcac ccactttgtc caccacaaaa ccagccgagg cctggcccag
      781 acccgacacc acaaaaaacg tgctcatcag caaaccgacc tgcgagtagc tcaggccaaa
      841 ctcggccatg aacaccggaa acatcagtgg cagcagcaaa tgaccaaagt gcgatgcggc
      901 atgcgccaga ccgaccagac caatgatggt ggcatcttgc ttgaaagaaa ctgacgaagg
      961 caaggaatgg gcggttgaag tcatgcgccc atcttgccag attcctgatt tgagtgcctg
     1021 gcccaggcgg ggctctataa atcaacctgt tgcagcgagc cagcctgacc ggattcgtac
     1081 aaaaaaacgc cggtactgcg caccagaccc aaactctgat tgctggccgt gttgacagaa
     1141 aacgggctgg cgacccggcc caggtacagc gcgccgacac cactttgggc caggctggtc
     1201 aagtggtcct gcccactgac atcacgttgc caaacccgca attggctaaa cactgcgtcg
     1261 ttgtcgtcga tccactggtt gccgtcctgg tcatatttgg ccaagtcggc aaaaccgtcg
     1321 ccgctgcgcg ggccaaacag ctcgctgcca tcgttgattt tgccgtcgcc atttttgtcc
     1381 agcgccagaa agccactgcc ccccgcgaca aaggcgatgt tttcctgctg accgtcggca
     1441 ttcaaatcaa acgcgaactg ggtatctgtg agtgccacac cggcaccgcc aaagttgatc
     1501 accagcgggt ctttgcgcac cgcgtcgcct gagcggatgg aaatgctgct ttcctgaaca
     1561 aatgatcggc tcatctccag ttgcaaggtg aaactgatct gctggccgtc agcggtttgc
     1621 accacgcctt gcgcactgac ctgggtggtt tcagactcgg cgattgtttg gcggctatca
     1681 agctcaaggc cccagcccgc cggggctgcc ggttgcgctg cggcggcagc ctggctgcct
     1741 gatgccccaa gctgaaccgg caggtcggca ttgctggccc cggattgcaa ttcacgggca
     1801 tcaaacaccc tgattctgcg tccggtgatg gactcaacca tgtcaatcag catggccagg
     1861 cgcgggtcgt tgcgcactgc cgcatcggcc tgcgccaccg catcggcttt tgcggtggct
     1921 gcatttgagg tcgccggtag ctggctctgc aagcgcttgc tggccgcttg ggacaaggtc
     1981 aatcggaccg cagcagcgtc ctgctgcacc gggcgctgac cttcaaaatc aggtcgctgg
     2041 ttgccaaccc aggcccgcat tgtggtatgt gatgtgcgca aagacacgct ggcgtgctga
     2101 ctttgcatgg cgatggtgga ttgagcgatc ttcatcgcca tttcatcgac caaaatgaaa
     2161 ggtaattcag ggtcttttca agctttattt ttcacccgct ttgccgaggc ccacccggca
     2221 cgaaaaagac agaaacaatc aggcaacgcc atgcgccttg aaccaggcca acgcacgttg
     2281 aaaggcatcg gcagcgtcgt ctttgcggta actggggcgg tagtccgaat gaaaagcgtg
     2341 cggagcgtct ttatagacca caaattgaga tgctttggct gctgcattgc ccttggcacc
     2401 cgcttcggcc agcgcgtctt tcatctggtt gatggtgaca agcggaatgc cgctgtcttg
     2461 cccaccatac aggcccagca ccggagcctt caggccagcc gccaaatcca gcggatgctt
     2521 gggggtcagg tccgaagcag tcccaaccaa tcggccatac caggcaacag ccgccttgac
     2581 gtttttgctc tgctcggcat acagccaggt aatgcgccca ccccagcaaa agccagtgat
     2641 tgccaccttt ttcagatcac caccattttc accagcccat ttgaccgcgc cgtccaaatc
     2701 acccatcact tgcgcatcgg gtaccttgga aaccagttca gccatcaatt tggcaacttg
     2761 ggtatacgaa gctgggtcac cttgacgcgc gaacaactca ggcgcgatgg ccaaatagcc
     2821 gacatgcgca aaacggcggc agatgtcggc aatgtgttca tgaaccccaa agatctcctg
     2881 aataaccagc accaccggca agttcttttt gccctcgggc atggcaaaga aaaacggcac
     2941 tgaaaagccc gtcatgtcgt aaacggattc acccgtgcgc agcccttcag aagatgtctt
     3001 gatcgcggtt tgcgccatga tgggcgcagc ggcagcggcg tagccaactc ccaatgcgac
     3061 tttcagcgcg gttcgccgac tcgcgccagc ctcgctggtc tggccgggca tcagcgcagt
     3121 gaaatcctga gtctgatcct tgttgagcat gggggtctcc ttgatttgaa aaaatgagag
     3181 cttagcgacg ctggaaaccc gcgtgatcca tggggtctta ccagtcacaa aatccgctat
     3241 gctctgcttt gcagttcatg cagacggtga tcccggcatg caaattgcgc gttccggtct
     3301 gttgcacggc tacccgccag gtaaccagcc ggttttatgg ttttgaagca agatacaagc
     3361 ttgccaggag actttttcat gccacagcgc caaacgcttc atctgcacgt gccagagccc
     3421 accgggcgac caggtcgcga caccgatttt tcctacctcc ccctgtctgc tgcgggcgcc
     3481 aagcgccgcc caccggtgga cgtggcagcc gctcagacca acgatctggc ctttgcgctg
     3541 atccgcgtgc tcgacgactc gggccaggcg ctcggcccct gggcaccgca gatggactcg
     3601 gccctgctgc gccgtggcct gcgtgccatg ctgctgaccc gggcctttga tgcgcgcatg
     3661 ctgattgccc agcggcaaaa aaaaatgtcg ttttacatgc agtgcctcgg cgaagaggcg
     3721 attgcctgcg cccacgcgct cgcgctgagt gaaggcgaca tgtgttttcc gacctaccgc
     3781 cagcagggtc tgctgctaac gcgcgaagac aacaacatgg tggacatgat ctgtcagctc
     3841 tacagcaacg agcacgaccc gatgaaaggg cggcaattgc cggtgatgta ttccagcaaa
     3901 aaacagggct ttttctcgat ttcgggcaac ttggccaccc aggtgatcca ggctgtcggc
     3961 tgggccatgg catccgccat caagggcgac agcaaggtcg catcaagctg gattggcgac
     4021 ggtgccaccg ccgaggccga ctttcatacc ggactgacct ttgcccacgt ctatcgcgcc
     4081 ccggtgattt tgaacgtggt gaataaccag tgggcaattt caacctttca ggccatcgct
     4141 ggcggcgaag gcaccacctt tgccgcacgc ggtgtcggca atggcatcgc ctcactgcgg
     4201 gtcgatggca atgactttct ggcggtttat gccgcttcac gctgggcgct ggaacgtgcc
     4261 cgcagcaact ttggcccgac cctgatcgaa tgggtgacct accgtgccgg gccgcattcc
     4321 acttcagacg acccgagcaa ataccgaccg gccaacgatg tcgcccgctt tcccctgggc
     4381 gacccgatag cgcgcctgaa gcagcacctg atcgtgctgg gcatctggtc tgacgctgag
     4441 cacgaggccg tgcaaaaaga ggtggaagcc gaagtggcag cggcccaaaa ggaagctgaa
     4501 aagtacggta ccctggccga tggcccaacc caaagcctgt cgggcatgtt tgaagacgtt
     4561 tacaaggaca tgcccgttca cttgcagcgc cagcgcgctg aactggaagg ccagtcatga
     4621 gcgatatgcc caacaccagc ccaatgacga tgatccaggc actgcgctca gccatggacg
     4681 tgatgctgga gcgcgacaac aacgtggtgg tctatggcca ggatgtcggt tactttggcg
     4741 gtgtcttccg ctgcaccgaa ggcttgcaaa agaagtacgg caaccagcgc gtttttgacg
     4801 cacccatttc tgagggcggc attgtcggca cagcggtcgg catgggggcc tacggcctgc
     4861 gcccggtggt cgaaatccag tttgccgact atgtctatcc ggccaccgat cagatcgtgt
     4921 cggaagcggc ccgcctgcgc aaccgctcgg caggcgactt tacctgcccg atcaccatcc
     4981 gcatgccctg tggcggtggc atttacggtg gccagacgca tagccagagc cccgaggcca
     5041 tgttcaccca ggtctgcggg ctgcgcaccg tgatgc
//
LOCUS       DAOSLL010000331         5070 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14738_length_5070_cov_4.304686, whole genome shotgun sequence.
ACCESSION   DAOSLL010000331 DAOSLL010000000
VERSION     DAOSLL010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5070)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14738_length_5070_cov_4.304686"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..834
                     /locus_tag="PLB25_13920"
     CDS             <1..834
                     /locus_tag="PLB25_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HOE42709.1"
                     /translation="NSLAPLHQPHNLEGIRAFRTAFAGLPQIACFDTAFHATLPEVDY
                     RFALPEELYLQGLRRYGFHGLSYQYVMGALLQHTPRATGRVLMAHLGNGASLCAAVDG
                     KSRATTMGFSALDGLMMGTRTGALDAGVLLYLLEHGWDHARLQKLLYKQSGLLGLSGI
                     SADMRRLRADPSPSAKLATDIFTYRLVRESGALVACIEGLDVLAFSGGIGEHDVLLRA
                     EMGQRLAWMGVAIDPQRNAQATADAVMSIHSATSRVEVWVVPTDEGVVAAREAARLIK
                     V"
     gene            891..1631
                     /locus_tag="PLB25_13925"
     CDS             891..1631
                     /locus_tag="PLB25_13925"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HOE42710.1"
                     /translation="MQLQDILYSQGFGTRRVCAGLIQQGHVQIYDKNQPPALVRQVQS
                     AIEIEANGLTFRVQGQDWPYAERAYIMLHKPPGTECSQKPSTYPSVYSLLPAPLRQRP
                     QKGAVQGVQAVGRLDQDTSGLLLLTDDGQFIHRMSSPKHHVPKVYEVNVKHPLDERQL
                     KRLLEGVLLDDDPRPVRAAACVAVSPQQLRLTLTEGKYHQVKRMLAAVGNRVEALHRS
                     QIGGLCLPDDLLPGQWRWLGAQDLLLLR"
     gene            1714..2709
                     /locus_tag="PLB25_13930"
     CDS             1714..2709
                     /locus_tag="PLB25_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HOE42711.1"
                     /translation="MTYFDHLKCRCRRQLAVLGLAAAALGAHAAPPVFVLNSLDATIS
                     VIDPVTWRETRRIAAGKEPHHLYMTPDEKSLIIANALSDSLMFVDPRTAEVQRTVKGI
                     LDPYQLRFSPDMKWFVTAANRLNHVDIYHWDGSNLNLAKRIVTGKTPSHLWIDSTSRT
                     IYSTMQDSDELVAIDLPTQALKWRHPTGSLPADVFGTPDDKFLLVGLTGGDGVEVYDV
                     TGAQPRLVKLLKTGKGAHSFRAVGDKRHVLVSNRVANTISKIDYQSMTVVDSYPGPSG
                     PDDMELSRDGRYIYVASRWAGKLSVIDTQTRTVLHQVKVGKSPHGVWTLDHAARN"
     gene            2750..3538
                     /locus_tag="PLB25_13935"
     CDS             2750..3538
                     /locus_tag="PLB25_13935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HOE42712.1"
                     /translation="MIAVSAHPSRATAQKACEKPVFLTFDTGHMGVAPLIAEVLERQH
                     VKVTYFAANERTQQGDGTLGQHWATWWKARADEGHEFASHTYDHVYWRADLPGATFRV
                     RPSAGALAGQDLNWSAVDYCAQLNQAHARLQQLTGKLPLPLFRAPGGKTSPQLLAAAK
                     ACGFAHVGWAPAGFLGDELPSEKYSNASLLAKALRDIRSGDILMAHLGIWSRQDPWAP
                     AVLEPLIVGLKQRGFCFATLREQPALRAWIARAAATAPLSVTAK"
     gene            3546..4502
                     /locus_tag="PLB25_13940"
     CDS             3546..4502
                     /locus_tag="PLB25_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOE42713.1"
                     /translation="MDWFAGQFSDLQGLLFESVLQPLMFNLGLGNFLVDGYTATGWLL
                     VGLLQLTVLLAVIGPLERWRPVEPVLDRAAVRVDVIYTLIHRLGLIRVALFFTLDPLF
                     DQLFGVLRVAGWHTFQLDAVWPGVTDLPWASLLLYLVVLDLVDYWLHRAQHQWRWWWS
                     LHALHHAQRQLTMWSDNRSHLLDDVIGAAVFVLIAQLIGIAPGQFVAIVALTQLSESF
                     QHANLRVWFGPVGERLWVSPRFHRLHHAVGIGHESRGRSTLGGYNFGVLLPYWDMLFG
                     TANFELRFDPTGVRDQVEQGRDYGHGFWSQQWLGLKRLAGRA"
     gene            4541..>5070
                     /locus_tag="PLB25_13945"
     CDS             4541..>5070
                     /locus_tag="PLB25_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EI24 domain-containing protein"
                     /protein_id="HOE42714.1"
                     /translation="MKLLFDSFWRAVAYCLRPRVIALSILPLLLMVGLALGLGYYYWD
                     MALDWVRGLVDASTFIDTVASWLQGLGLGGLKAALVPLIVIFAITPLIVVLSLLVVAL
                     LMTPALTALVAERRFPSLERKHGNSFLSGLLWSLFSALLALVALVVSVPLWLIPPLIL
                     ILPPLIWGWLTYRVMA"
BASE COUNT          870 a         1480 c         1581 g         1139 t
ORIGIN      
        1 aactctttgg cacccttgca ccagccgcac aacctggagg gcattcgcgc ttttcggaca
       61 gcgttcgcgg gcttgccgca aatagcatgt tttgacaccg cctttcacgc cacgctgccc
      121 gaagttgatt accgctttgc cctgcctgaa gagttgtacc tgcaaggcct gcgccgctac
      181 ggctttcacg gcctgtcgta tcagtatgtg atgggcgcgc tgttgcagca tacgccgcgc
      241 gcgaccggtc gggtgttgat ggcgcatctg ggcaatggtg cgagtttgtg cgcagcggtc
      301 gatggcaaga gtcgtgccac caccatgggt ttttctgcgc tggatggcct gatgatgggt
      361 acccgcaccg gcgcgctcga cgccggcgtg ttgctctatt tgctggagca tggctgggac
      421 cacgcccgtc tgcaaaagct gctttacaaa caaagcggct tgctgggcct gtcgggcatt
      481 tcggccgaca tgcgccgttt gcgtgctgat cccagcccca gtgccaaatt ggccaccgac
      541 atttttacct accgcttggt gcgtgaaagt ggtgcgctgg tggcatgcat agagggcctg
      601 gatgtgctgg ctttcagtgg cggcattggc gagcatgatg tgctgttgcg ggccgaaatg
      661 gggcaaagat tggcctggat gggcgtggcc attgacccgc agcgcaatgc gcaagccact
      721 gctgacgcag tgatgtcgat tcattcggcc accagccggg ttgaagtctg ggtggtgccg
      781 accgacgaag gtgtggtggc agcgcgtgag gcggcccgcc tgatcaaagt ctgaagcacc
      841 gggtccaccc ggtgcaggtg tcgcaaccga attagcggta cctctccagc atgcagttgc
      901 aagacattct ttattcccag gggtttggca cgcggcgcgt ctgtgccggt ctgatccagc
      961 agggtcatgt tcagatttat gacaaaaatc agcctccagc ccttgtcaga caagtgcagt
     1021 cagctattga aatagaagca aatggattga ccttccgggt tcaggggcaa gactggccgt
     1081 atgccgagcg cgcctacatc atgctgcaca agccaccggg caccgagtgt tcgcaaaaac
     1141 catcgaccta ccccagcgtc tatagcctgt tgccagcacc gctgcgccag cgaccacaaa
     1201 agggtgcagt gcagggcgta caggcggtcg ggcgactcga ccaggacacc agcggtttgt
     1261 tgctgctgac cgatgacggt cagttcatcc accgcatgag ttcgcccaag caccatgtgc
     1321 ccaaggtcta tgaggtgaac gtcaagcacc cgcttgatga gcgccagctc aagcggctgc
     1381 ttgaaggcgt gctgctcgac gatgacccca ggccagtgcg ggcggcggct tgcgtggcgg
     1441 tgtcgccgca gcaactgcgt ctgaccctga ccgagggcaa ataccaccag gtcaagcgca
     1501 tgctggcggc agtcggcaat cgggttgaag cgctgcaccg ctcgcagatt ggcggcttgt
     1561 gcctgcccga cgacctgttg ccggggcaat ggcgctggct gggggcgcag gatttgctgc
     1621 tgctccggta gccccttgcc agacagcggc gacaaactgg cgcgttacac tttgcggctg
     1681 ctgttcctga tgcaattttc tgttgaccca cccatgactt atttcgatca tcttaagtgt
     1741 cgttgccgtc gccagttggc cgtgcttggg ttggcagcag cagcactggg cgcgcatgcg
     1801 gcaccgccgg tgtttgtgct caattcgctt gatgccacaa tcagcgtgat tgaccccgtt
     1861 acctggcgcg agacccggcg tattgcggca ggcaaggagc cgcatcacct gtacatgaca
     1921 ccggatgaaa agtcgctgat cattgccaat gccttgagcg attcactgat gtttgtagac
     1981 cccaggaccg ccgaagtgca gcgcaccgtc aaaggcattc tggaccccta tcagttgcgc
     2041 ttttcgcctg acatgaaatg gtttgtcacg gctgccaacc ggctcaatca tgtggacatt
     2101 taccattggg atggcagcaa tctgaacctg gccaaacgca ttgtgaccgg caagacgccc
     2161 agccacctct ggattgacag caccagccgg acgatttatt cgaccatgca agacagcgat
     2221 gagctggttg caattgactt gccgacccag gcactcaagt ggcgccaccc caccggttcc
     2281 ctgccggccg acgtgtttgg cacgccagac gacaaatttt tgctggtcgg actaaccggc
     2341 ggcgacggtg ttgaagttta tgacgtgacg ggtgcgcagc cgaggttggt caaattgctg
     2401 aaaaccggca agggagccca ttcatttcgc gccgttggcg acaaacgcca tgtgctggtc
     2461 agcaaccggg ttgccaatac catcagcaag atcgattacc agagcatgac ggtcgttgat
     2521 agctatcctg gtcccagcgg tcccgatgac atggaactgt cgcgtgacgg tcgctacatt
     2581 tatgtggctt cgcgctgggc tggcaagctg tctgtgatcg acacccagac ccggaccgtg
     2641 ctgcatcagg tcaaggttgg caagtcgccc catggagtct ggacactcga ccatgccgcc
     2701 cgcaattaag gtcgcacgcg catttcaggc catcttttta gctattttta tgatagctgt
     2761 cagcgctcat ccatctaggg ctacagccca aaaagcttgt gaaaagccgg tttttttgac
     2821 attcgatacc ggccacatgg gcgtggcccc gctgatcgcc gaagtgcttg agcgccagca
     2881 tgtcaaggtg acctatttcg ccgccaacga gcgcacccag caaggcgacg gcacgcttgg
     2941 gcagcactgg gccacctggt ggaaggcgcg tgccgacgag ggccatgagt ttgcctcgca
     3001 cacctatgac catgtgtatt ggcgcgccga cctgcctggt gcgacgttcc gggtgcgccc
     3061 cagtgccggt gcgctggccg gacaggatct gaactggagc gctgtcgact actgcgcgca
     3121 actcaaccaa gcccatgcgc gactgcagca gctcaccggc aagctgccgc tgccgctgtt
     3181 tcgtgcgccg ggcggcaaga cttcgcccca attgctggcg gctgccaaag cctgcggctt
     3241 tgcccatgtt ggctgggctc ctgccggatt tctgggcgac gagttgccca gtgaaaaata
     3301 cagcaacgcc agtctgctgg ccaaggcctt gcgtgacatc cgttcgggcg acatcctgat
     3361 ggcgcatctg ggcatctggt cgcgccagga cccctgggca ccggcggtgc tggagccgct
     3421 gatcgtcggg ctcaaacaac gcggcttctg ttttgccacc ttgcgcgagc agccagcttt
     3481 gcgcgcctgg atcgcccggg ctgctgccac tgcaccgctg tccgtcactg cgaaatagct
     3541 gggccatgga ctggtttgcc gggcagtttt ccgatcttca ggggctactg ttcgagtccg
     3601 tgctgcaacc cttgatgttt aacctggggc tgggcaattt tctggtggac ggttacaccg
     3661 ccaccggatg gctgctggtc ggtctgctgc aactgacggt cttgctggcg gtgatcgggc
     3721 cgctggagcg ctggcgaccg gttgagccgg tgctggaccg ggcagcggtg cgggtcgatg
     3781 tgatttacac cctgatccac cggctcggcc tgatccgggt ggcgctgttc tttacccttg
     3841 atccgctgtt tgaccagctt tttggtgtgc tgcgggtggc cggttggcat acctttcagc
     3901 ttgatgcggt gtggcctggc gttaccgatc tgccctgggc aagtttgctg ctgtacctgg
     3961 tcgtgctgga cctggtggac tattggctgc accgcgccca gcaccagtgg cgttggtggt
     4021 ggagcctgca tgcactgcac catgcacagc gtcagctcac catgtggagc gacaaccgca
     4081 gccacttgct tgacgatgtc attggcgctg ccgtctttgt actgatagcc cagctcatag
     4141 gcatcgcgcc cggccagttt gttgccattg tggcgcttac ccagctcagc gagagctttc
     4201 agcatgccaa cctgcgggtc tggttcggtc ccgtcggcga gcgcctgtgg gtcagcccgc
     4261 gctttcaccg gttgcaccat gcggtcggca tcgggcatga atcgcgcggc aggagcaccc
     4321 ttggcgggta caactttggt gtcttgctgc cgtactggga catgttgttt ggcactgcca
     4381 atttcgaact gcgctttgac ccgaccggtg tgcgcgacca ggtcgagcaa gggcgtgact
     4441 atggccacgg cttctggtcg caacagtggc tgggcctgaa gcgactggcc ggacgggcct
     4501 gacagaagca agccatacgg cagtcagtta tgcttgaccc atgaagctgt tgtttgactc
     4561 tttttggcgc gctgttgcgt attgcctgcg gcctcgggtg atcgcgctgt caattctgcc
     4621 gctgctgttg atggttgggc tggcgctggg gcttggctac tattactggg acatggcgct
     4681 cgattgggtg cgcggtctgg ttgatgcctc caccttcatc gacactgtgg catcctggct
     4741 gcaaggtctg ggactggggg gcctcaaagc tgcgctggtg ccattgattg tgatttttgc
     4801 catcacgcca ctcattgtgg tgctgtcgct gttggtggtg gccttgctga tgacccctgc
     4861 tttaaccgcg ctggtggctg agaggcgctt tccatcactt gagcgcaagc atggcaattc
     4921 ttttctgtca ggcctgctgt ggtcgctgtt ttcagccctg ctggcgctgg tggcgctggt
     4981 ggtgtcggtg ccgctgtggc tgattccgcc cttgatcctg atcctgccgc cgctgatctg
     5041 gggctggctg acctaccggg taatggcgtt
//
LOCUS       DAOSLL010000332         5053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14801_length_5053_cov_4.141857, whole genome shotgun sequence.
ACCESSION   DAOSLL010000332 DAOSLL010000000
VERSION     DAOSLL010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14801_length_5053_cov_4.141857"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PLB25_13950"
     CDS             complement(<1..397)
                     /locus_tag="PLB25_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42715.1"
                     /translation="MPTLPTAAGSALSHFDARPFFEKALQYGVQHGIIGQAKLDAMAQ
                     EAPKGMVQIARYFGSEYLRPELETARERIVNMVSLSLEDSSGGDLREAACLLRDHSFL
                     SRSKAGSDLLKALIVMPQNTHFGMNERGEF"
     gene            complement(427..1173)
                     /locus_tag="PLB25_13955"
     CDS             complement(427..1173)
                     /locus_tag="PLB25_13955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42716.1"
                     /translation="MNHLALIEKTQSLIAAGDILGAESALVELADKEGDGALVEVLAQ
                     LPAKDLLAVIREYDNSKSSIVGLALTAQQFARAVVIEKQYRDLTRTHLRGMMNSVIFR
                     DGADPIEFLTAIGDLEGGSEALADYFEEKWSRIEAFARSGSFDAAEDDGDLVAEDDLR
                     ANAYARARMEQDEVADQDWMQLAWLLRYEIPDLFIEMLLVLRAKARAFDLGVADDYLP
                     EDDGKVETRDTDLGKATPAALESDDEESAI"
     gene            complement(1215..1784)
                     /locus_tag="PLB25_13960"
     CDS             complement(1215..1784)
                     /locus_tag="PLB25_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42717.1"
                     /translation="MSMSAFFRALRTSYQSEIDDLTFDSEGRDVLRRRLLEKRSQIAF
                     LSQMIESSPEMVAVVFHGGFHFELPAVMEQVLTLESDEFPDWDSLADAVQLTPWARDL
                     AKLFLKEPQGAWFMTVAAVLEYLYHRAPPHHQDPAQGLPHDGQTTPTGGRHLDHGHDF
                     DADDESHQQHDHEETSAEWLEDQGFDRKD"
     gene            complement(1810..2085)
                     /locus_tag="PLB25_13965"
     CDS             complement(1810..2085)
                     /locus_tag="PLB25_13965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HOE42718.1"
                     /translation="MKDHYAVLGLRSSASLQEIKIAFRQQASRHHPDRNDAPDAAARF
                     RLVQEAYEVLADAGKRKAYDDNRQRNLLDSPIDTARAIWLDYFGRLL"
     gene            2688..3746
                     /locus_tag="PLB25_13970"
     CDS             2688..3746
                     /locus_tag="PLB25_13970"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOE42719.1"
                     /translation="MTFPNALENLNVLAQQTLLPPSRLHDAVPASDRATHTVSLARAT
                     VADILKGQDPRLLVVVGPCSIHDIIAAMDYAQRLKALADELSDQLFIVMRVYFEKPRT
                     TVGWKGLINDPHMDDSFRIEEGLHLARKLLVDLNHMGLPCGTEALDPITPQYLGDLIA
                     WSAIGARTTESQTHRELASGLSSPVGFKNGTDGNLEVALNAMLAAAQPHAFLGINGEG
                     QVALTLTRGNAFGHLILRGGSNPNYDSVAVAQAEADLVAARLPTNIVVDCSHANSRKN
                     HALQSLVLKDVVGQIVDGCRSIKGVMLESNLFEGNQKLGDPKQLRYGVSITDACLGWE
                     ATAVGLREAAQRLRTASR"
     gene            complement(3788..4273)
                     /locus_tag="PLB25_13975"
     CDS             complement(3788..4273)
                     /locus_tag="PLB25_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HOE42720.1"
                     /translation="MTTVYDFEALSIDGKPVALNQYKGKALLIVNTASACGFTPQFSG
                     LEKLHESYGKQGLVVLGFPCNQFGSQDAGSNDEIASFCQLNYGVTFQMMQKIEVNGEQ
                     AHPLYRWLSSEAPGLLGSKSIKWNFTKFLVGKDGAVRKRYAPTDTPESLTKDVEAALA
                     A"
     gene            complement(4365..>5053)
                     /locus_tag="PLB25_13980"
     CDS             complement(4365..>5053)
                     /locus_tag="PLB25_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HOE42721.1"
                     /translation="MRPMPKKIITLLPDFWSCNVRLWRDRLGQISLAATTLIWGVAGN
                     LRFIVLAWAAAALGYSVTQASALQGVVAIGMALGAVVASMRMRLEDGPRVITLGIGMG
                     LLIILLIFIDNVWIAAPFLIMLGALGGFLVVPMNALLQHRGHNLMGAGRSIAVQNFNE
                     QACILGLGALYSLSTGLGLPTFGAITMFGLVIASFMWVIQRWHARNCRTHGPEVNRLL
                     AIARNDKAHG"
BASE COUNT         1071 a         1517 c         1447 g         1018 t
ORIGIN      
        1 caaattcacc gcgctcattc atgccaaagt gggtgttttg tggcatgaca atcaatgcct
       61 tgagcaggtc cgagccggcc ttggatcgtg acagaaagga atggtcgcgc agcaggcacg
      121 ccgcctcgcg caaatctccg ccgctgctgt cctcaaggga caggctgacc atattgacga
      181 tacgttcgcg tgcagtttca agctccgggc gcaggtactc gctgccaaag tagcgcgcaa
      241 tctgcaccat gcccttgggt gcctcctgtg ccatggcatc gagtttggct tgaccaatga
      301 tgccgtgctg cacgccgtac tgcagcgctt tctcaaaaaa aggtcgcgca tcaaagtgtg
      361 aaagggcgct accagcagcg gtcgggagcg ttggcataca gcgcaattga gcgttagcga
      421 agccgttcaa atggctgatt cttcgtcatc cgactccagc gcggctggtg tggctttgcc
      481 caggtcggtg tcacgggttt cgaccttgcc gtcgtcttcg ggcagatagt cgtccgccac
      541 gcccaggtca aaggcacgcg ccttggcccg cagcaccaac agcatctcaa tgaacaggtc
      601 agggatttcg tagcgcagca gccaggccag ttgcatccag tcctgatcgg ccacctcatc
      661 ttgctccatg cgtgcgcgcg cataggcatt ggcacgcaaa tcgtcctcag cgaccaaatc
      721 cccgtcgtct tctgccgcat caaaactacc cgagcgcgca aaggcttcga tgcgactcca
      781 cttttcttca aaatagtcag ccagtgcctc gctgccaccc tcgaggtcgc cgattgcagt
      841 caaaaattca attgggtctg caccatccct gaagatgacc gaattcatca tgccgcgaag
      901 gtgcgtgcgc gtcaggtcgc ggtactgctt ttcgatcacc acagcgcggg caaactgctg
      961 tgctgtcagt gccaggccga cgatggatga tttcgagttg tcgtattcgc ggatcactgc
     1021 caacagatct ttggctggca actgcgccag cacttccacc agtgcgccat cgccctcttt
     1081 gtcggccagc tcaaccagtg ccgattcggc ccccaaaatg tcgcccgcag caatcagcga
     1141 ctgggttttt tcaatcaatg ccagatgatt catgtcgaga atgcgtccta tggatagtgg
     1201 cgtgcgcttt gggctcagtc cttgcggtca aaaccttgat cttcaagcca ttcggcgctg
     1261 gtttcttcgt ggtcatgctg ctgatggctt tcgtcatccg catcaaagtc atggccgtgg
     1321 tcaaggtggc ggccgccggt gggggtggtt tgcccgtcat gcggcagccc ttgggcaggg
     1381 tcctgatggt gtggtggcgc tcggtggtac aaatactcca gcactgctgc caccgtcatg
     1441 aaccaggccc cttgtggctc tttcaagaac agcttggcga ggtccctggc ccaaggcgtc
     1501 aactgcaccg catctgccag gctgtcccag tccggaaatt catcggattc aagcgtcagc
     1561 acctgctcca tgacggcggg caactcaaaa tggaacccgc catgaaacac caccgccacc
     1621 atttcggggc tggactcgat catctggctc agaaaagcga tttgactgcg cttttccagc
     1681 aggcgccgac gcaacacgtc gcggccttcc gaatcaaatg tgaggtcatc aatctctgac
     1741 tgataggacg tgcgcagtgc gcgaaaaaaa gctgacatgc tcatggcatt ccttagagtc
     1801 aggccgccgt caaagcaggc ggccaaaata atcaagccag atcgcacgcg cagtgtctat
     1861 cgggctatcg agcaggttgc gttgccggtt gtcgtcatag gctttgcgtt tgccagcatc
     1921 agccaacacc tcgtaagcct cttgcaccag gcgaaagcgg gccgctgcat ccggggcatc
     1981 gttgcgatcc gggtgatgcc ttgaggcctg ctggcgaaac gcgatcttga tttcttgcag
     2041 acttgcagaa ctgcgcaggc ccaatacggc gtaatggtct ttcatgactg gcagagatca
     2101 caaatcaggc agtagccaac atgggcattg ggctgtttgc cataaaggtt gggacaatag
     2161 caacaaaaca ccatcaattt ttgaagtgtc cctgctggtt gggatttgac acagtgcgat
     2221 tgtaggtggt tcgcaacaaa ttccaaaaga atgcaggcgg cagtcaactg ttgccctgac
     2281 ggtggttttg acggcttcag gggccgttgt ggagcgacct ggtcgccagc ctgcaggtta
     2341 accagtgtga gcgcggcggt cgaggtgtcg cgcaaggcac ccggtgcgcc agtggctgcg
     2401 ctgtgtcgtc gttgcggcca ggccacaaca gcatgttgtt gcctgtcggg atcatgggta
     2461 gctattaaaa tagtagcttc cagtgcagat agaataaggg ctggagccca taaacctatc
     2521 agacttatgg cgtatcggct gcgatacctg ccgcagtggc ccgttttgca ccggcttttt
     2581 cacccgtagc tggcgatggg cctgaaaata ccgcacccat cactggggtc gattcgcgct
     2641 gtcaagccct atgtccgaca ggcacccaat tcaccaattt atttgccatg actttcccaa
     2701 atgcccttga aaacctgaac gtgttggcgc agcaaacttt gctgccacct tcccggctgc
     2761 atgacgcggt acccgccagc gaccgtgcaa cccacaccgt cagcctggca cgcgccacgg
     2821 tagccgacat tctcaaaggg caagacccgc gcctgctggt ggtggtgggg ccatgctcga
     2881 tccacgacat cattgcggca atggactatg cgcagcgcct gaaagcgctc gccgacgagt
     2941 tgtcagacca gcttttcatc gtcatgcgcg tctatttcga gaagccccgc acaaccgttg
     3001 gctggaaagg gctgatcaac gacccgcaca tggacgactc gtttcgcatt gaggaaggcc
     3061 tgcacctggc ccgcaagctg ctggtggact tgaaccacat gggcctgccc tgtggcaccg
     3121 aggcgctaga cccgatcacg ccacagtacc tgggcgacct gattgcgtgg agcgcgattg
     3181 gggcgcgcac caccgagagc cagacgcacc gtgagctcgc cagtggcctg tcgagcccgg
     3241 tgggtttcaa gaacggcacc gatggcaacc tggaagtggc gctcaatgcc atgctggcag
     3301 cggcccagcc acatgccttt ttgggtatca acggggaggg tcaggtggcg ctcaccctta
     3361 ccaggggcaa tgcttttggt cacctgatct tgcgcggtgg cagcaacccc aattacgact
     3421 cggtagcggt ggcgcaagcc gaagcagacc tggtggccgc gcggctgcca accaacatcg
     3481 tggtggactg cagccacgcc aactcacgca agaaccacgc gctgcaaagc ctggtgctca
     3541 aggacgtggt gggccagatc gtcgacggtt gccgctccat caagggcgtg atgctggagt
     3601 ccaacctgtt tgagggcaac cagaaacttg gtgacccgaa gcaactgcgc tatggtgtgt
     3661 cgattaccga tgcctgcctg ggctgggagg ccaccgctgt cggcttgcgc gaggcagctc
     3721 agcggctgcg tacagccagc cgataggcca cagttggcgc ggggatgccc ggtctcggga
     3781 aaggccgtca ggcggccaga gccgcctcaa catctttcgt caggctctca ggggtgtcgg
     3841 tgggggcgta gcgcttacgt accgcgccgt ccttgccgac cagaaacttg gtgaaattcc
     3901 acttgatgga tttgctgccg agcaggccgg gtgcctcgct cgacagccac cgatagagcg
     3961 gatgggcctg ttcgccgttg acttcaattt tttgcatcat ttgaaaagtc acgccatagt
     4021 taagctggca aaagctggcg atctcgtcat tgctgccagc gtcctgcgaa ccaaactggt
     4081 tgcacggaaa ccccagcacc accagcccct gcttgccata gctttcatgc agcttttcca
     4141 agccggaaaa ctgtggcgta aagccacagg cgctggcggt gttgacgatc agcagtgctt
     4201 tgcctttgta ctggttcagg gcaaccggct tgccgtcgat ggacagcgcc tcaaagtcat
     4261 aaacagtggt catcaccagc ctctcagatg aatatcaatg gcaatatggg aagatttcgg
     4321 caccaatgta agccaacgcc aatccccttg ccgttggcat gtgatcagcc gtgcgccttg
     4381 tcgttgcggg caatggccag caggcgatta acctcgggac catgcgtgcg gcaattgcgc
     4441 gcatgccagc gctgaatcac ccacatgaag ctggcaatca ccagcccgaa catggtgatg
     4501 gcaccaaagg ttggcagccc cagcccggtg gacaggctgt agagcgcgcc caaacccaga
     4561 atgcaggcct gctcattgaa gttttgcacg gcaatcgacc gacccgcgcc catcaggtta
     4621 tggccccggt gctgcaacag cgcgttcatc ggcaccacca gaaaaccacc cagcgcgccg
     4681 agcatgatca gaaacggcgc agcaatccag acattgtcga taaagatcag caagatgatc
     4741 agcaagccca tgccaatgcc cagggtgatc acccgcgggc cgtcctcgag ccgcatgcgc
     4801 atcgaggcca ccacggcacc cagagccatg ccaatcgcca ccaccccttg caaggccgag
     4861 gcttgcgtca cactgtagcc aagcgcggcg gcagcccagg ccagcacaat gaaacgcaag
     4921 ttgccggcca cgccccagat caaggtggtg gcggccaatg aaatctgccc cagccggtcg
     4981 cgccacaaac gcacattgca cgaccagaaa tcaggcaaca aggtgatgat ctttttcggc
     5041 atgggccgca tca
//
LOCUS       DAOSLL010000333         5052 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14804_length_5052_cov_3.920953, whole genome shotgun sequence.
ACCESSION   DAOSLL010000333 DAOSLL010000000
VERSION     DAOSLL010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14804_length_5052_cov_3.920953"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(172..1209)
                     /locus_tag="PLB25_13985"
     CDS             complement(172..1209)
                     /locus_tag="PLB25_13985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HOE42722.1"
                     /translation="MRYRLLGQTGLYVSELCLGTMTFGDQGFWKVMGGLGQDAATALV
                     KQAFDAGVNFIDTANIYSLGEAEQLTGTAIKSLGLPRDELVIATKATGVMNERAVNAL
                     GQSRYHLMNELDASLKRLQLDHIDLYQLHGFDPLIPLEDVLSTLNDMVRSGKVRYIGL
                     CNMAAWQIMKALAISVKNHWARFESVQAYYTVAGRDLEREILPLLQDQQLGLMVWSPL
                     AGGLLSGKFNPDGTGPQGTRRASFDFPVVDKPRAFRVVDAMRPIAAQHQVSVAQVALA
                     WLLSRPQVSTVIMGAKTPAQLEDNLGASKLELSAQNLRDLDEVSALPLEYPGWMLERQ
                     GQSRAKPPEKP"
     gene            complement(1298..1921)
                     /locus_tag="PLB25_13990"
     CDS             complement(1298..1921)
                     /locus_tag="PLB25_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42723.1"
                     /translation="MAISLQPADRMTQTILNLISKVPFSSEAVSSTPRARASQLARSA
                     ARTSAGISGGAALVPGPLGMLTLLPDLFGVWRVQAQMVCDIAGAYGKSATLTQEQMVF
                     CLFKHSAAQMLRDVIVREGTRYLVLPASLRMMQSLATRIGIQIGQRSVGKAIARYAPL
                     VGALGTGAYAYYDTRQVADTAIELFSSEIVVQSGGPDNSGARAAVVN"
     gene            2884..3960
                     /locus_tag="PLB25_13995"
     CDS             2884..3960
                     /locus_tag="PLB25_13995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013209950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HOE42724.1"
                     /translation="MLFESFDLGGIRLSSRTVMAPMTRSRATHNNTPDALIAEYYKQR
                     ATAGLIVTEGTSSSPNGLGYARIPGIYNEEQVLGWRLVTDAVHQAGGKIFVQLMHTGR
                     VSHQNNLPAGARVVGPSPEVCPGEMWTDKAGLKPHTAPVVMTEADIAQAVAEYANAAK
                     LAMKAGFDGIELHAANGYLIEQFLNANINKRTDGYGGSTQARNRFAIEVAEACAGAIG
                     ADKVGIRLSPYGVFNGTGAFEGVDDQFLSLTKKLSELGLIYVHLVDHSGMGAPPVPAE
                     LKKAIRAAFGRTYIAVGGFAAQSAEAVLQAKEADLIAFGRPFISNPDLVARMKSGAQL
                     TVPDMSTFYTPGEKGYTDYPAVVA"
     gene            4235..4510
                     /locus_tag="PLB25_14000"
     CDS             4235..4510
                     /locus_tag="PLB25_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42725.1"
                     /translation="MNEEQLQCELDSLVATQAGLMAIVGSLMATHPDYEKFQLHLTGL
                     LEVLLTGDAGKTFTQRQRLQAREFVETLQHLNKAPAKIEPLGTMPNL"
BASE COUNT         1178 a         1413 c         1361 g         1100 t
ORIGIN      
        1 acgagcacct ggggtttcaa gtagagcggc gtaacccgtg gtgccttcct tgcagccaca
       61 gaatcgggct cagcagcatg agccacagat cgcgttcgca gtctcgcatc aaagtggcag
      121 ggcagggcgg aggtcagccc aagctcaagc acactcgaag catgacccag ctcagggttt
      181 ttccggtggt tttgcccggc tttgcccctg tctttcgagc atccagccgg ggtattccag
      241 tggcaaggcg ctaacttcgt caagatctcg caagttctgt gcgctcaact caagcttgga
      301 ggcacccagg ttgtcctcga gttgcgctgg tgtttttgct cccatgatga cagtgctgac
      361 ctgtggacgg ctcagcaacc aggccaaggc gacctgcgca actgatactt gatgctgcgc
      421 agcgatcggg cgcatcgcat caacgacgcg aaatgcccga ggcttgtcaa ccactgggaa
      481 atcaaaactg gcacggcgtg tgccttgtgg ccccgttccg tcagggttga acttgccgct
      541 gagcagacca ccggccaaag gactccagac catgaggccg agctgttggt cctgtagcaa
      601 aggcaagatc tcgcgttcga ggtcacgccc ggcgacggtg taataggctt gcacgctttc
      661 aaaccgagcc caatgatttt tcacggaaat cgccagggcc ttcatgattt gccaggccgc
      721 catattgcac aggccgatgt agcgcacctt gccagagcgt accatatcgt tgagcgtgga
      781 cagcacgtct tccaatggga taagggggtc aaagccatgc agttggtaca agtcaatgtg
      841 gtcaagttga aggcgcttga ggctggcatc gagctcgttc atgaggtgat agcgtgattg
      901 cccaagggcg ttgacggccc tctcattcat gacacctgtg gccttggtgg caatgaccag
      961 ttcgtcccgt ggcagcccaa gtgacttgat agcagtcccg gtcagttgtt ctgcttcacc
     1021 cagagaatag atattggcgg tatcgatgaa gttgaccccg gcatcaaaag cctgtttcac
     1081 cagggcggtg gccgcatcct gccccagtcc acccatgacc ttccagaaac cctggtcgcc
     1141 aaaagtcatc gttcccaggc acagttcaga aacatacagg ccggtttggc ccaataggcg
     1201 atagcgcatg gcatcctctt gaaaaaatta ttctgaaaaa aacgtcgtga tgcagtggct
     1261 ccctgcattt gaggtctcca actgccgaaa atcaaattca attcaccaca gcagccctgg
     1321 cacctgaatt atcaggacct ccactttgca ccacgatctc gctgctgaac aactctattg
     1381 cggtgtcagc cacttgacgt gtgtcatagt atgcataggc tccagtgccc agtgctccaa
     1441 ccaggggcgc atatcgcgca atcgccttac caacactgcg ttgaccaatc tgaatgccaa
     1501 ttcttgtcgc cagactttgc atcatccgca agcttgcagg caggaccaag taccgtgtgc
     1561 cttcacgcac gatgacatct cgcaacattt gcgcagcaga gtgtttgaac aggcaaaaaa
     1621 ccatctgctc ctgagtcaag gttgccgact tgccgtacgc accggcaatg tcgcagacca
     1681 tctgtgcctg aactcgccac acaccgaaga ggtctggcag caaagtcagc atgcccaaag
     1741 ggccagggac caatgcggca ccaccagaaa taccggcgct ggttctggcc gcactgcggg
     1801 ctaactggct tgcgcgtgcc cgaggagtgg aactgacggc ctctgaggag aatggcacct
     1861 tcgaaatcag attcaggatg gtttgcgtca tccgatcagc gggttggagg cttatcgcca
     1921 tttgattccc taaagttggc atccgacagc gcaacgacaa aaatgcacag cgtcatcggt
     1981 aggtgttgga catgatggca ttgaaactca cgctggagct tgcatgcatc ccatattgga
     2041 tgatgaatta gccactgaat gataccgaaa tcaggtgtct gtcaccaatc gcgcaggctg
     2101 actccccaag cttctatcat tgcacaatgc cagccctcgt tcatcacttc gataccccat
     2161 cttcggttca ggaatggctt tgcatcaacg gtggggtcat gggcggcgat tttgagcagt
     2221 tgcctttgct tcgatgcggg cgggcatgca gtgctcgaag gcgaggtcgc tcttgaaaat
     2281 aatggcggct ttgtttcggt gcggacatcg tcaattcgag tgggtgatcc cggcaactgc
     2341 gtacgaggtg ccttgcgcgc cttctcacat ccaacttttc ataactggtg tctttgcata
     2401 tcgccacaaa ccggcagaag acatcactcc agggcaggcg atcattcaat ttggcgtgga
     2461 aagttgctgc tctgccaaag gttggcggca atactcgcct gaccgttgat ttccctcggt
     2521 ttgggcaagt cccgttaaca gatcaatgta aacgtcgtta agactcaata gagtagaaaa
     2581 ggcttcgtgg attttggtcg ccactgatcc aggcttgggt cctggaatct ttgcgcatcc
     2641 gccgttgcaa agtcaaatga cggtcatcat ggcatggcaa aatgcccgcc aagcttgcgc
     2701 ttgacgaggg atggcgtggc cacagcgcag tcgcttgtat tggatgccga attcgaagac
     2761 aacttgcaaa gcaggcaatc accctcaact gcggcctcgc tgtgagacat tcgcacagat
     2821 gtgtctgcca acaatggcca tctcattacc agccaaacaa caaaccaact tttagggcaa
     2881 ccgatgctat ttgaatcttt tgacttgggc ggcatccgcc tgtccagccg cactgtgatg
     2941 gctccaatga ctcgcagccg cgcaacacac aacaacacac ctgatgcgct tattgcagag
     3001 tattacaaac aacgtgcgac cgccggtttg atcgtcaccg agggtacttc atcgtcacct
     3061 aatggtcttg ggtacgcccg gattcccggc atctacaacg aagagcaggt cctgggctgg
     3121 cgattggtca cggacgcagt ccaccaagct ggtggcaaga tatttgtgca actcatgcac
     3181 acggggcgtg tttcgcacca gaacaaccta ccagccggtg cgcgagtcgt cggccccagc
     3241 ccagaagtct gccccggtga aatgtggacc gacaaggctg gcttaaaacc acatactgcg
     3301 cctgtggtaa tgaccgaggc tgacattgcg caagcagttg cggaatatgc caacgccgcc
     3361 aagctggcca tgaaggcagg atttgatggc attgaactcc acgctgccaa cggttatctg
     3421 atagagcaat tcctgaacgc taatatcaac aagcgcaccg atggctacgg cggcagtacc
     3481 caagcccgca atcgttttgc cattgaagtc gctgaagcct gtgcaggagc aattggagcc
     3541 gacaaagtgg gcattcgtct gtccccctat ggtgttttca acggaacagg tgccttcgaa
     3601 ggcgttgacg atcagttcct gtcgctgaca aagaagttgt cagagttggg gctgatctat
     3661 gtgcatttgg ttgaccattc cggcatgggg gcacccccgg tacctgctga actcaagaaa
     3721 gcaattcgag cggcctttgg cagaacatat atcgccgtcg gtggattcgc tgcccagagc
     3781 gctgaggctg tcttgcaggc caaagaggca gacctcatcg catttggtcg cccctttatt
     3841 tcaaaccccg acttggtcgc tcgaatgaag tcaggcgctc aattgacagt acccgacatg
     3901 agtacgtttt acacgccggg cgaaaagggc tatacagact accccgcagt ggtagcctaa
     3961 atcgcctgca gatggcaacg aggcttgtca ggcgccattt ttgggcgttc agtcggactt
     4021 aggaaccttg ccgcatttga cgaggagggc ccgtctgaag cgccgaccat gggatacacc
     4081 aacaaaatcg gtgtgctgct gcctctgcga acgcctgcct gaccaacgcg tacaagcgct
     4141 tgcccaagtc aggtagtcgc tcaatgccgc aagcattatt tgacaaagaa atcgaatctc
     4201 ttggaaatca actatggcga ccaaggacgt caacatgaat gaagaacaat tgcagtgcga
     4261 actcgacagc cttgtcgcca ctcaagcagg actcatggca attgtcgggt cactcatggc
     4321 cacgcacccc gattacgaaa agtttcagct tcatctgact ggacttttgg aagtgctgct
     4381 gaccggcgat gctggaaaga ccttcacgca aagacagcgt ctacaggcac gtgagtttgt
     4441 tgaaacgctt cagcacctca acaaggcacc cgcaaaaatc gagccgcttg ggacgatgcc
     4501 caatctgtag tgatcgttgc ggataggagc ctgcgcgacg cgcccgagtc cgcgttgtgt
     4561 gcaccgcagg ttcgacggtt tgcccacagt cgtactgttg tggccgggca aaacaaccca
     4621 gatccaattg gcatctagag ctcggacagg atgcccacaa tcgctgcgct cagtatggac
     4681 aagtccgagg cgctgtagcc gcgcctcccc gctacgcggg tccccatccg gcttgtttta
     4741 aagaaatcac ccaatccaag gatggcgcaa gcgcctacca gcctacaagt tggacactga
     4801 aatgtctgtg cgtcggccgc caacgaacaa gcaaacaagt gcgcaggcag tgggtcagat
     4861 tactgtgagc acaggttggg cagtttccgt gagcgccaaa agtgaggcga tcacaccaag
     4921 ttcgtgcatc atcgcctgta tcgtttgccc cttcataggt gagcgcttgc caagtctcga
     4981 agtgttcggc aatgtctcaa ctggacagtg ccaagttctc cccgttattc aggcggtttc
     5041 ttggccaggc gc
//
LOCUS       DAOSLL010000334         5044 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14829_length_5044_cov_4.766286, whole genome shotgun sequence.
ACCESSION   DAOSLL010000334 DAOSLL010000000
VERSION     DAOSLL010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14829_length_5044_cov_4.766286"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PLB25_14005"
     CDS             <1..556
                     /locus_tag="PLB25_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3362 domain-containing protein"
                     /protein_id="HOE42726.1"
                     /translation="IAAHPGTTDEDMMNLAIWLKKNGFRADQVQTFYPSPMATATAMY
                     HTGRNPLRGVHRDLQGDDESVDVVRGERRRRLHKAFLRYHDANNWPLLREALKAMGRA
                     DLIGNGKQHLIPAWQPLADAGYQSARKKNSTPSRLKRLPPDAEQANKPSAVVQGRILT
                     QHTGLPPRVSGAGGKPASLRRKPR"
     gene            682..981
                     /locus_tag="PLB25_14010"
     CDS             682..981
                     /locus_tag="PLB25_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42727.1"
                     /translation="MTMFDCGIDGEEKFKDSDGKASGKKMVAQLKNAGQCPRTHKDDG
                     REAFDVTNERHLQYLTRPLAILKMALPVLRCWLGVSAGEPQFARRMRRKVLSPWM"
     gene            complement(933..1478)
                     /locus_tag="PLB25_14015"
     CDS             complement(933..1478)
                     /locus_tag="PLB25_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007827762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HOE42728.1"
                     /translation="MTLRTISDRYHRSSIALHWLMLALFIAVYASINLRELFDKGTAP
                     REALKSLHFMLGLLMFVLVWLRLAMRIKHPAPGIAPAPPAWQAAAAKIAHLALYAIMI
                     GMPVLGWLLLSAAGKPIPFFGLELPALITPDKALASQLKEVHEFIGELGYFLIGAHAA
                     AALFHHYIQGDNTLRRMRLAN"
     gene            complement(2212..3597)
                     /locus_tag="PLB25_14020"
     CDS             complement(2212..3597)
                     /locus_tag="PLB25_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011869997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HOE42729.1"
                     /translation="MQALNLSELIGALSYALDITEGQPEGHCIRCCWIGMHIGHKLNL
                     PKDQLWELYYTLLLKDLGCSSNASRICELYLTDDLAFKRDFKWVDGSLPQVLRFVLRH
                     TGPGAGLMDRLKAMGGILTNGDEIANELTKSRCTRGADIARQLRFSEGVAQGIHSLDE
                     HWNGHGRPEGLAGQQIALYSRIALLAQVVDILNSSGGQAAAEAEVQQRSGNWFDPEVV
                     AAFLAIAHDARFWHTLNGTDLETHVFAMEPGGHESELDEDYLDDIAEAFGQVVDAKSP
                     YTAGHSARVGLYTALLGESMEIAESRRRWLKRGALLHDVGKLGVSNTILDKPGKLDEG
                     EWAVMRKHPVHTESILGRIKNFDELARVSAAHHERLDGGGYPRGLKAHDIALETRIIT
                     TADIFDAITAERPYRGAVPVPKTLAIMADSVGTAIDARCFEHLQQLIKRSPELFPGQV
                     LSKRRIGNSTA"
     gene            3778..4371
                     /locus_tag="PLB25_14025"
     CDS             3778..4371
                     /locus_tag="PLB25_14025"
                     /inference="COORDINATES: protein motif:HMM:NF025596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4338 domain-containing protein"
                     /protein_id="HOE42730.1"
                     /translation="MQPDGFTSATNLSQLHVRLVERSEEPRYQDQMALHHYLGGLSKI
                     GETLWYAVIWGEQWVALLSISAAALKCGVRDRWFGWNFTFQYGRLKLVANNSRFLILP
                     EWHHPNVVSRVLSLMQRRLVSDWRARFGHPVLLLETFVDPERFYGDVCRATNSTELGL
                     TQGNRRTRAGYSEQHQAPKWVFADTIRSNPHKKQILC"
     gene            4571..4858
                     /locus_tag="PLB25_14030"
     CDS             4571..4858
                     /locus_tag="PLB25_14030"
                     /inference="COORDINATES: protein motif:HMM:NF025180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HOE42731.1"
                     /translation="MLTADQMRILPDFFNDIADPRARSGRRHRLSTVLSIAAAATLCG
                     MKGYKVMAGWAQDLGEKARARLGCRRVDGKYVVPSESIFCDVLIRVNPAVQ"
BASE COUNT         1127 a         1474 c         1407 g         1036 t
ORIGIN      
        1 catcgccgcc caccccggca ccaccgatga agacatgatg aacctggcca tctggttgaa
       61 aaaaaacggc tttcgcgccg accaggtgca aactttctac cccagcccga tggccaccgc
      121 caccgccatg taccacaccg gtcgcaaccc gttgcgcggc gtgcaccgcg acctgcaggg
      181 cgatgacgag tcggttgatg tggtgcgcgg tgaaaggcgc cgccgtctgc acaaggcctt
      241 tttgcgctac cacgatgcca acaactggcc gctgctgcgt gaagcgctca aggcgatggg
      301 ccgcgctgac ctgattggca atggcaaaca gcacctgatc ccggcctggc agcccctggc
      361 agatgccggt taccagagtg cgcgcaagaa aaattcaacg ccatcgcgtc tcaagagatt
      421 gccgcccgat gcggagcagg caaataagcc atcagcagtg gttcaaggcc gcatcctgac
      481 gcagcacact ggtctgccgc cacgggttag tggagccggt ggcaaaccgg ccagcttgcg
      541 ccgcaagccg cgctgatatt gcgcccagcc aagcggctgg cttcaggtca gttaaccatc
      601 tgagcgcagc agcaggttgg cgcataagcc gccacgacat gatggctatt gctggcccaa
      661 gcaaaccaga tcctttgccc tgtgaccatg ttcgactgtg gcattgatgg cgaggaaaag
      721 ttcaaggaca gcgacggcaa agccagcgga aagaagatgg tcgcgcagct caagaatgca
      781 ggccaatgcc cgcgcaccca taaggacgat ggcagggaag catttgacgt gacgaacgaa
      841 cgccaccttc aatatctgac caggccgctg gccatcctga agatggcatt gccggtactt
      901 cgctgctggc ttggggtttc ggcgggtgaa cctcagtttg ccagacgcat gcgccgcaag
      961 gtgttgtcgc cctggatgta gtggtgaaac agggccgctg cggcgtgcgc cccgatcaga
     1021 aagtaaccca gctcgccgat gaattcgtga acctccttga gctggctggc cagggctttg
     1081 tccggtgtga tcagcgctgg cagctccagc ccgaaaaatg gaattggctt gcctgcggca
     1141 ctgagcaaca accagcccaa aaccggcatc ccgatcatga tggcgtacag cgccaggtga
     1201 gcaatcttgg ccgccgccgc ctgccaggcc ggtggcgctg gtgcaatacc gggggctggg
     1261 tgtttgatgc gcatcgccag acgcaaccag accagcacga acatcaacaa tcccagcatg
     1321 aaatgcaggc ttttcagggc ctcgcgcggc gcagtgccct tgtcaaacaa ttcccgcaaa
     1381 ttgatgctgg catacacggc aatgaacagg gccaacatca accaatggag cgcaatggaa
     1441 gagcggtggt agcggtcaga aatggtgcga agtgtcatct tttaagatcc agtaattggt
     1501 aggtgacgaa ctgtaaaaag ccaggattaa ccctgtctta acaaccaaca ccgccgttct
     1561 taagtaccca acgtttcaaa atttgattcc aataaggcac aatttctcgc tccaaagcat
     1621 gcaatttcat cccccaatta ccgctgacgg catgactttt cggccattct gaatccagca
     1681 gacgacaaac aatgccctac aaactggcca acgctgcgca acaacttgcc caattgcgtc
     1741 gatccgctca ggattgccac aagttcccca cctgttacgc accacactga tctagccaaa
     1801 cgaacccagg caattgcagt ttgaatgtcc catggccatt tgggaagtgc aaaagtcgca
     1861 cgaagatcgg gacattggca gaggccggaa ttgttcaaga tggtgatgct tttgggctcc
     1921 ggggcgatgc tcacggtaca caaaaatggc caggctggca tccggcagcg caacctgcac
     1981 ctggctttga tgtcgatcag cgcgccaatt ggtcagtgtg cgacacagcg atcggttggg
     2041 cttggggact tgccaaggcc cccattccat agacgcgctg cggggtaagc cgcgtgcgcg
     2101 ccagaccaga agcagaccag cgcccaactg acttgccatt gagcgcttgc gacggttcaa
     2161 actacacagg tcaaagatgt ttgggagccc aaaacccgtg ccaacttgct ttcatgcggt
     2221 tgagtttcct atccgtctct ttgataacac ctgcccaggg aaaagctccg ggcttctctt
     2281 gatgagctgc tgcaagtgtt caaaacagcg ggcatcaatc gctgtgccca ccgagtcggc
     2341 catgatggcc agcgtttttg gcaccggcac tgcaccccgg tagggtcgct cggcggtgat
     2401 ggcatcaaaa atgtcagccg tggtgatgat gcgggtttcc agtgcgatgt cgtgcgcctt
     2461 cagccctctc gggtaaccac cgccatcgag ccgttcgtgg tgcgccgccg aaacgcgagc
     2521 caactcatca aagttcttga tgcggcccag aatcgattcg gtgtgcaccg ggtgctttcg
     2581 catgacggcc cattcgccct cgtcaagttt gccgggtttg tccaagatgg tgttgctgac
     2641 accgagcttg ccgacatcgt gcaacagtgc gccacgcttg agccagcgcc gacgcgattc
     2701 cgcaatctcc atcgactcac ccagcagggc tgtgtaaagg ccgacccggg cactatggcc
     2761 tgcggtgtac gggctcttgg cgtccaccac ctggccaaag gcttcggcaa tgtcgtccag
     2821 atagtcttcg tcaagctctg attcatggcc gccgggctcc atagcgaaga catgcgtttc
     2881 aagatcggtg ccgtttagcg tgtgccagaa cctggcatca tgggcaatcg caagaaaagc
     2941 agcgaccact tcggggtcaa accagttgcc gctacgctgt tgcacttcgg cttcagcggc
     3001 cgcttgaccg ccagatgagt tgagaatgtc caccacctga gccagcaaag caatgcgcga
     3061 gtacagtgca atctgctggc ccgccagccc ttcagggcga ccatgcccgt tccagtgctc
     3121 gtccagcgaa tgaatgccct gtgccacgcc ctctgaaaaa cgcaactgtc gggcaatgtc
     3181 tgcgccccgg gtgcagcggg atttggtcag ttcgttggca atttcgtcgc cgttggtcag
     3241 gatgccgccc atggccttaa gtcgatccat caacccggct cctgggccgg tgtggcgtaa
     3301 cacaaagcgc aagacctgtg gcaggctgcc atcaacccat ttgaagtcgc gcttgaaagc
     3361 taagtcatcg gtcaggtaca gttcgcagat acgcgaggcg ttgctgctgc agcccaagtc
     3421 tttcagcagc aaggtgtagt aaagctccca gagctggtct ttgggcaggt tcagcttgtg
     3481 gccaatgtgc atgccaatcc agcaacagcg tatgcaatgt ccctccggct ggccttcggt
     3541 gatgtccaat gcatagctga gcgcgccgat tagctctgat aaattcaatg cttgcatgtg
     3601 tggcttctct gcggcaatcg aggtacaaag gatgttcagt ttaagacttg tcaacggata
     3661 aggctagggt tgatgtcagt gccaggatga ttttgatcgc tgatagatgt tgtttgaaat
     3721 caacagtgtc acgcttaacg ctgaacatcc caccttatac cccttacaaa cacggctatg
     3781 cagcccgatg gattcacaag cgcgacaaat ctttcacaac ttcacgttcg cctggtagag
     3841 cggtctgaag agccacgcta ccaagatcaa atggcgctac accactacct cgggggtctg
     3901 tcaaagattg gcgagacgct gtggtatgcg gtcatctggg gcgagcagtg ggtagccctt
     3961 ctgagcatct ctgctgcagc cctcaagtgc ggcgtacgcg accgctggtt tggctggaac
     4021 ttcaccttcc agtatggccg cctcaagctc gtcgctaaca acagccgttt tctgatcctg
     4081 cccgagtggc accaccccaa tgtcgtttca cgtgtgctgt ctttgatgca acgccgcttg
     4141 gtgagcgatt ggcgggcacg ctttggtcac cctgtactac tgctggaaac ctttgtggac
     4201 cctgagcgct tttatggtga tgtttgccgc gccaccaact cgacagaact cggtctgact
     4261 caaggcaacc ggcgcacccg cgcaggctac agcgaacagc accaggcgcc caagtgggtc
     4321 ttcgctgaca ccatacgcag caaccctcac aagaaacaga tattatgtta acaatcaaaa
     4381 atatagctag taacgcagtc tgcataaggg ctggatgccg atttggcaga taaaaccctg
     4441 gcagcttgcg ccgtcggcct agcccatgtt cctttgagaa cgcctgcccc gcgtgcgggg
     4501 ttaggatgct caagagccat taactcaagc tgatggagcg caacttcaac tcacaggcaa
     4561 acccaacatc atgctcacag ccgatcagat gcgcatactg cccgacttct tcaacgacat
     4621 cgccgacccc cgcgcgcgct caggtcggcg ccaccgtttg tcgacggtgt tgagcattgc
     4681 cgcagctgct acgctgtgcg gcatgaaggg ctacaaggtc atggcaggct gggcgcaaga
     4741 ccttggagag aaggcgcgcg ctcgcttggg ctgtcgtcga gtagatggca aatatgtggt
     4801 gcccagcgag tccatcttct gtgatgtgct catccgggtg aatccagcag tgcagtgacc
     4861 aacaattagt tttttaaaaa aaccatgatc cactgggaat ctcacgtctg tctgccattg
     4921 catccgcagg cgagctttgg tccgctggag cgcctgcgcg cacagggggt gcactatgcc
     4981 agcgtcaatg tgggcatgga tatgaatccg ctgacgcaaa tcctgtcagt gattgcgggt
     5041 tacc
//
LOCUS       DAOSLL010000335         5040 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14841_length_5040_cov_3.737011, whole genome shotgun sequence.
ACCESSION   DAOSLL010000335 DAOSLL010000000
VERSION     DAOSLL010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14841_length_5040_cov_3.737011"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..613
                     /locus_tag="PLB25_14035"
     CDS             <1..613
                     /locus_tag="PLB25_14035"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HOE42732.1"
                     /translation="VLNKPGKLTQAEFEIMKTHPTEGYKMLMASTGVEPMSLDVVLHH
                     HEKMDGSGYPECFKGDQISLHGKMAAVCDVYDAITSNRPYKSGWDPAESLRKMAEWAN
                     GHFDPKIFQAFVKSMGIYPVGSLVRLTSGRIGVVTEQSKRSLTTPLIKVFFSTKSNMR
                     IVPVMLDLSHPSATERIQQREDPNKWNFPDINELWSGLSHLGR"
     gene            complement(728..1996)
                     /locus_tag="PLB25_14040"
     CDS             complement(728..1996)
                     /locus_tag="PLB25_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOE42733.1"
                     /translation="MDFDYSPKTKQLQAKLLAFMDEHIYPAEGAHHAEVEANTLAGRR
                     WTPLQTIENLKLKAKAQGLWNLFLPVDSAQAAGFDGAGLSNQEYAPLAEIMGRVMWCS
                     EVFNCSAPDTGNMETIARYGADEHKQQWLRPLLEGEIRSAFSMTEPDVASSDATNIET
                     RIERDGDGYLINGRKWWISGAGDPRCAIHIVMGKSDPAAQRHLQQSMILVPANAPGVK
                     ILRALNVLGYDDAPHGHMEISYQNVRVPASSILLGEGRGFEIAQGRLGPGRIHHCMRL
                     IGLAERSLELMCRRAASRIAFGKPLAAQTVTQERIAEARCKIDMARLLTLKAAWMMDQ
                     AGNKFAKNEIAMIKVVAPNMACEVIDWAMQVFGAAAMCDDFPLAYAYACARTLRFADG
                     PDEVHRNAIAKLELRKYSAQPAAVEAGFLS"
     gene            complement(2161..3246)
                     /locus_tag="PLB25_14045"
     CDS             complement(2161..3246)
                     /locus_tag="PLB25_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HOE42734.1"
                     /translation="MSNFDDFNGTRAVPEAHSFDQPALTQWLTQHLDGFVGPMQVESF
                     KGGQSNPTYKLITAHQTYVMRAKPGPVAKLLPSAHAIEREYAVMAGLAGSAVPVPQML
                     CLCEDESVIGRAFYIMSFVEGRIYWDQSLPGMTPAQRGQIYREMNRVMAALHSVDFVA
                     AGLGSYGKPGNYFERQIARWSKQYTASITQPIVEMDQLMAWLLDNMPAMARDPSIVSI
                     VHGDFRLDNLVFHPTEPRVIAVLDWELSTLGHPLADFSYHCMGWHIAPGAFRGIGGLD
                     LQALGIPSESEYIRQYCERTSFASPEQLANDWRFYMIYNLFRLAAILQGIGKRIESGT
                     AASAQAISAAAGAKPLAQMAWQFAQRS"
     gene            complement(3320..4039)
                     /locus_tag="PLB25_14050"
     CDS             complement(3320..4039)
                     /locus_tag="PLB25_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOE42735.1"
                     /translation="MTEPILTIEERAAINAGRWFASLSPSLRHDVLRCAYVRRYTDSE
                     LISARGDQPQEWIACAKGAVRVSSTSISGKQVTLTYVEPGIWFGDVAIFDGDRRTHDA
                     YAHGDTTILCVARADFTKIMGLHPELCEALLRLQARRIRQLYGLVEDLNTLPLRARLA
                     KQLLHLARSYGVPTPGDDAEVRIGLHLAQEELAQLLGASRQRVNQELKAMERDQSIRL
                     DPTGLVIRNREALLQLIEADK"
     gene            complement(4114..>5040)
                     /locus_tag="PLB25_14055"
     CDS             complement(4114..>5040)
                     /locus_tag="PLB25_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HOE42736.1"
                     /translation="EKINNTVKGLGQLSVRLKDADVIILNRERTILSRPLIEKLPKLK
                     LVVQTGRIGAHIDIDACTERGVAVADGTGSPVAPAELAWALIMAAARRLPQYIGNLKH
                     GAWQQSGLKSSGMPPNFCLGTTLKGRTLGIWGYGKIGQLVAGFGQAFGMQVLIWGRPP
                     SLERALQDGYRTTSSREELFEQSDVLSLHLRLTEETQAIVRLEDLSRMKPSALLVNIS
                     RAELIEPDALLGALNRGRPGMAAIDVFESEPILQGHALLRLENCICTPHIGYVEQDSY
                     ELYFGAAFDNVVNFIKGRPTNIVNPGALQVRR"
BASE COUNT         1028 a         1457 c         1519 g         1036 t
ORIGIN      
        1 ggtgctgaac aagccgggca agttgacgca ggccgagttt gaaatcatga aaacccaccc
       61 gaccgagggt tacaaaatgc tgatggccag caccggggtt gagccgatgt cgctggacgt
      121 ggtgctgcat catcacgaaa aaatggacgg gtcaggctac ccggagtgtt tcaagggcga
      181 tcagatcagc ctgcacggaa aaatggcagc cgtgtgcgat gtttatgatg ccatcacctc
      241 aaaccggccc tacaagtcag gttgggaccc ggccgagtcc ctgcgaaaga tggccgagtg
      301 ggccaacggc catttcgatc cgaagatttt tcaggctttt gtcaagagca tgggcatata
      361 cccggtgggc tcgctggtgc gcctgacctc gggacgcatc ggcgtggtca ccgaacagtc
      421 caaacgatca ctgacaacgc ctctgatcaa ggtgtttttt tctaccaaat cgaacatgcg
      481 catcgtgcca gtgatgcttg atttgtcgca ccccagcgcc accgaacgta tccagcaacg
      541 cgaggacccg aacaagtgga acttcccgga tatcaatgaa ttgtggtcag ggttaagcca
      601 tctcgggcgc tgacaggctt gcgcgttggt tgctgtcggc tcaacttggc cagtctgttg
      661 gccggggtcg atgtcaggca agctgggaaa gcaaatgcag caaggacatg caaatgcaag
      721 ccagccgtca actgaggaat ccggcttcaa ccgctgctgg ctgggctgag tacttgcgca
      781 gttcgagctt ggcgatggcg ttgcgatgca cctcatcggg gccgtcggca aaacgcaggg
      841 tgcgagcaca ggcataggca taggccagcg gaaagtcatc gcacatggcg gcagcaccaa
      901 aaacctgcat cgcccagtcg atcacctcgc aggccatgtt gggtgccacc accttgatca
      961 tggcgatttc atttttggcg aacttgttgc cagcctggtc catcatccag gcagccttca
     1021 gtgtcagcag gcgtgccatg tcgatcttgc agcgggcctc ggcaatgcgc tcctgggtca
     1081 cggtctgtgc tgccagtggc ttgccaaagg cgatgcgact ggcggcgcgt cggcacatca
     1141 attcgagcga gcgctcggcc aggccaatca ggcgcatgca gtggtgaatc cgcccaggcc
     1201 ccaggcggcc ctgggcgatc tcaaagccgc gtccttcgcc cagcaaaatg ctgctggctg
     1261 gcacccgcac gttctggtag gaaatttcca tgtggccatg cggtgcgtcg tcataaccca
     1321 atacgttgag cgctcgcagt atcttgacac cgggggcgtt ggcgggcacc aggatcatgc
     1381 tctgctgcag gtggcgctgt gccgccgggt cagatttgcc catgacgatg tgaatggcgc
     1441 agcgcgggtc gccagcaccg ctgatccacc atttgcggcc attgatcaga tagccgtcac
     1501 cgtcacgctc aatgcgggtt tcaatgttgg tggcatcgct cgaagccacg tcgggctcgg
     1561 tcattgaaaa ggccgagcgg atttcgccct ccagcaaggg ccttagccac tgctgcttgt
     1621 gttcatcagc gccatagcgt gcgatggttt ccatgttgcc ggtgtcgggt gccgagcagt
     1681 tgaacacctc gctgcaccac atcacccgac ccatgatttc ggccagcggc gcgtattcct
     1741 ggttgctcag gccagcgccg tcaaagccgg ctgcctgtgc gctgtcaacc ggcaaaaaca
     1801 ggttccacag gccttgcgcc ttcgccttga gcttcaggtt ttcgatggtt tgcaacgggg
     1861 tccagcgccg gccagccagc gtattggctt cgacctcggc atggtgagca ccctcggcgg
     1921 ggtaaatgtg ctcgtccatg aacgccagca gcttggcctg aagttgtttg gtcttggggg
     1981 agtagtcaaa atccatgctg gcctcgtcaa tcaaaatggg gtggagtcgg ctggcttgca
     2041 gcgacgcgtc cggatcgggc aatctgatgc gctgcgatgc cacctggcgt gatcgtcatt
     2101 tgtgtatcaa atagggctgt agcccttgtg caataagcgc aacgtgctat aaaaaataga
     2161 tcaactgcgc tgcgcaaact gccacgccat ttgtgccagt ggcttagccc cggccgccgc
     2221 actgatggcc tgggcactgg cggccgtgcc agattcaatg cgtttgccga tgccttgcaa
     2281 aatggcggcc aggcgaaaca ggttgtagat catgtaaaag cgccagtcat ttgccagttg
     2341 ttccgggctg gcaaagctgg tgcgctcgca gtattggcgg atgtactcgg actccgaagg
     2401 aatacccagg gcttgcaaat cgagcccacc gatgccacga aacgcacctg gcgcaatgtg
     2461 ccagcccatg cagtggtagc tgaaatcagc cagcgggtgc cccaaagtgg acagttccca
     2521 gtccagcact gctatgacgc gtggttcggt cgggtgaaac accaggttgt caagccggaa
     2581 gtcgccgtgc acgatgctga caattgacgg gtcgcgtgcc attgctggca tgttgtcgag
     2641 taaccaggcc atcaactggt ccatttcgac gattggctgg gtgatggatg ccgtgtactg
     2701 cttgctccag cgggcaatct ggcgctcgaa atagttgcct ggcttgccat agctgcccag
     2761 gcctgccgcc acgaagtcaa ccgaatgcag cgctgccatt acccggttca tttcccggta
     2821 aatttggccg cgctgcgcgg gtgtcatgcc gggcagggac tgatcccaat agatgcgccc
     2881 ctcgacaaaa ctcatgatgt agaaggcccg accgatgacg gactcgtcct cgcacaggca
     2941 cagcatttgc ggcaccggta cggctgagcc tgccagcccg gccatgaccg catattcgcg
     3001 ctcaatcgca tgggctgaag gcagcagttt ggccaccgga ccaggtttgg cgcgcatcac
     3061 ataggtctga tgggcggtga tgagcttata ggttgggttg gactggccac ctttgaaaga
     3121 ttcaacctgc atcgggccga caaatccatc gagatgctga gtcagccatt gggtcaaggc
     3181 tggttgatcg aatgaatgcg cctcaggtac ggcacgggtg ccattgaagt cgtcaaaatt
     3241 gctcatggat accccatcag gtcaggtttg atgcagcaag ctgcgttgtg ccatctgggg
     3301 cgcaagaccc aaggcggcgc tatttgtcgg cttcgatcag ttgcagcaag gcttcgcggt
     3361 tgcgaatcac caggccggtg gggtcgaggc gaatcgattg gtcgcgctcc atcgccttga
     3421 gttcctggtt gacgcgctgg cgcgaggctc ccagcaactg tgccagctct tcctgcgcca
     3481 gatgcagccc aatgcgcacc tcggcatcgt cgcccggtgt cggcacgcca tagctgcgcg
     3541 ccaggtgcag cagttgcttg gccagccgcg cccgcagtgg caaggtgttg aggtcttcga
     3601 ccaggccgta gagctggcgg atgcgccgag cctgcaggcg caacagggct tcgcagagtt
     3661 cgggatgcag ccccatgatc ttggtgaagt cggcccgggc gacgcacaaa atcgtggtgt
     3721 cgccatgcgc ataagcatcg tgggtgcgcc ggtctccatc gaagatggcc acatcgccaa
     3781 accagatgcc cggctcgaca taggtcagtg tgacctgctt gccggaaatc gaggtggaac
     3841 tcacccgcac tgcgcctttg gcgcaggcaa tccactcctg aggctggtcg cctcgggcgg
     3901 agatcagttc cgaatctgta taacggcgaa cgtaagcgca tcgcaggaca tcatgtctga
     3961 gagagggtga aaggctggca aaccagcgcc cggcattgat tgccgcgcgt tcctcaatgg
     4021 taaggatggg ctctgtcatg acctgtcatg ttggtgactg aaaacctctg gaatgtcacc
     4081 tgtgcgacgc aggcatgaca ccagaccgac cggttaacgc ctgacctgca acgcacccgg
     4141 gttgacgatg ttggtcgggc gacccttgat gaagttcacc acattgtcaa aggcggcacc
     4201 aaaatacaat tcgtagctgt cttgctccac gtagccgatg tggggtgtgc aaatgcaatt
     4261 ttccagccgc agcagcgcgt ggccctggag aatcggctcc gactcaaaca cgtcgattgc
     4321 cgccatgccg gggcgaccac gattgagcgc gccgagcagc gcgtctggct caatcagttc
     4381 ggcccgagaa atgttcacca gtagcgcaga tggtttcatg cgggaaagat cctccagccg
     4441 cacaattgcc tgcgtttctt ctgtcaggcg aagatgcagc gacagcacat ctgactgctc
     4501 aaacaactct tcacggctgc tggtggtccg gtagccgtct tgcaaggcac gttcgagcga
     4561 aggcgggcga ccccagatca gcacctgcat gccaaacgcc tggccgaagc cagcgaccag
     4621 ttgaccaatc ttgccgtagc cccaaatacc cagggtgcgc cccttgagcg ttgttccaag
     4681 gcaaaaattc ggcggcatgc cacttgattt caggccagac tgctgccacg caccatgttt
     4741 gaggttgcca atgtactgtg gcaggcgtcg cgctgcagcc atgatcagtg cccatgccaa
     4801 ctcagccggt gccaccggtg agccggttcc gtccgcaaca gcgacacctc gttcggtgca
     4861 agcgtcgatg tcaatgtgtg cgccgatgcg tccggtctgt accaccagct tgagtttggg
     4921 caatttttca atcagtgggc gcgagagaat ggtgcgctca cggttcaaaa tgatcacatc
     4981 agcgtctttg agccgaaccg acaactggcc cagccccttg actgtgttgt ttatcttctc
//
LOCUS       DAOSLL010000336         5030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14877_length_5030_cov_4.046030, whole genome shotgun sequence.
ACCESSION   DAOSLL010000336 DAOSLL010000000
VERSION     DAOSLL010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14877_length_5030_cov_4.046030"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            359..1720
                     /locus_tag="PLB25_14060"
     CDS             359..1720
                     /locus_tag="PLB25_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOE42737.1"
                     /translation="MKPLPELMSFLEGLPEPHILFDTQYRILAANAAYRCQFSPDRSV
                     VGRHCYEVSHHFSVPCDQSGELCPLVNARQSGQRERVLHLHHTARGEEYVNIELAPLL
                     DGNGDLSFFVEKVESMRVAQGRAGAQGLIGRSVEFQHMLAFVARVAPSMATVLLLGES
                     GTGKELVASAVHVASARAGRALVPVDCSSLAENLFESELFGHERGAFTGATTARTGLV
                     EAASGGTFFLDEVGDIPLTMQVKLLRLLETGTYRRVGSTEQRHADIRVVSATHRNLDQ
                     MVAQGRFREDLYYRLSTFPIHLPALRERRDDIALLAASLLTRVAPARKLRLTDAALAL
                     LQTQNYPGNVRELRNLLERSALMCDGDSLDATHVEQALRSGRRPNAAAPEHNNNATPV
                     TDQAVANAALPVAAPGALKSVERAALHEMLKAHPGSRAELARQLGISERSLYRKIKTL
                     NAP"
     gene            complement(1905..2306)
                     /locus_tag="PLB25_14065"
     CDS             complement(1905..2306)
                     /locus_tag="PLB25_14065"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HOE42738.1"
                     /translation="MGAQKYVRINDCEGTVTELSLFVTRLRTGMGEEIAIPNALVAGN
                     VTRNFSRIRNSAGRSGYVLDTTVTIGYDTPWRQVHSMLIEAASNPVPPCADQSGQNRS
                     AWWSGSPTIRLEALRESYSNPLAVARLQCQS"
     gene            2295..3082
                     /locus_tag="PLB25_14070"
                     /pseudo
     CDS             2295..3082
                     /locus_tag="PLB25_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584370.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(3423..4013)
                     /locus_tag="PLB25_14075"
     CDS             complement(3423..4013)
                     /locus_tag="PLB25_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42739.1"
                     /translation="MKKTLLALAISIGVVAATLGTPALAKSIPAKGADGNWISAKSDD
                     AGNNTFSFREFSFDGNNWNVKYTLYLDEAKTMPVFVFRGIGTYSVTGKSKKVKGASEA
                     LFLFDKKTLTLMTDNADLINNLGFANCKLEKGVERDISETGCSFVAPLAKCNREYDLL
                     KVVAKKLFLGARPADNNMCTLDKRPNALGDTLIRKE"
     repeat_region   4424..5029
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=4569..4603
                     /rpt_unit_seq="gtttcaatccacgcccgccattgctggcgggcgaa"
BASE COUNT         1021 a         1493 c         1446 g         1070 t
ORIGIN      
        1 gctcggcgca aatctggcca ccaacggcac cgcagttgct gccgccgcac cgaccaccac
       61 ggccgtcagc accacctaat gatgccgctt ggcatcgtgc aaacaggcat cgggtgccaa
      121 gacatcaaag gaatcagaat ggttcatgag tgtccccaga aggccataag gagttgatga
      181 gccaactatc aaaatagcgt gctatcgccg ggaattcaaa aaacctcttg aattcagcca
      241 gttatgtttt cttcagacga atgccattga ttagatcaga caccaactgc caagaatgac
      301 agtctgtcag tctattgaca tacactcgct ggcagtttga cttctggttc gcagccgcat
      361 gaaacccttg cccgagttga tgtcctttct ggaaggcttg ccagagccgc atatcctgtt
      421 cgacacccaa taccgcattc tggcggccaa tgcggcctac cggtgccagt tcagcccgga
      481 ccgcagtgtg gtcggccgcc actgctacga ggtgtcacac catttcagcg tgccgtgcga
      541 ccagtccggc gaattgtgtc cgctggtcaa tgcgcgtcaa tctggtcaac gcgagcgtgt
      601 gctgcacctg caccacaccg cccggggcga ggaatacgtc aacatcgaac tcgccccgct
      661 gcttgatggc aacggcgatc tgtctttttt cgtcgaaaaa gtggagtcaa tgcgggtggc
      721 gcagggccgc gctggcgcac agggcctgat tggccgctcg gtcgagttcc agcacatgct
      781 ggcctttgtg gcgcgggtgg caccgtcgat ggccaccgtg ctgctgctgg gcgaatccgg
      841 caccggcaag gaactggtag ccagcgcagt tcatgtggcc agcgccaggg cgggccgggc
      901 gctggtaccg gtggactgct caagcctggc ggaaaacctg tttgaatccg aactctttgg
      961 tcatgagcgc ggtgccttta ccggtgccac cacagcgcgc actggtctgg tggaagcggc
     1021 cagcggcggc accttttttc tcgacgaagt gggcgacatt ccgctgacca tgcaggtcaa
     1081 gttgctgcgc ctgctggaaa ccggcaccta ccggcgcgtt ggcagcaccg aacagcgcca
     1141 tgccgacatt cgggtggtgt cggccaccca ccgcaacctt gatcaaatgg tggcacaggg
     1201 gcgctttcgt gaagacctgt attaccggct cagcactttt ccaatccacc tgcctgcgct
     1261 gcgcgaacgc cgtgatgaca ttgccctgct ggcggcatcg ctgttgaccc gcgttgcacc
     1321 agcacgcaaa ctgcgcctga ccgatgccgc gctggcactg ctgcagacgc agaactaccc
     1381 cggcaatgtg cgtgaattgc gcaacctgct ggaacgctcg gcactgatgt gcgatggcga
     1441 cagcctggat gcaacccatg tcgagcaggc gctgcgttca ggtcgtcgcc cgaatgccgc
     1501 tgcgccggag cacaacaaca acgcaacgcc agtgaccgat caggcagttg ccaacgctgc
     1561 cctgccggtt gccgcaccgg gcgcgctgaa atcggtcgaa cgcgccgccc tgcacgagat
     1621 gctcaaggcc cacccgggca gccgagccga actggcccgg cagttgggca tcagcgagcg
     1681 atctctgtac cgcaagatca agaccttgaa tgcgccgtag tcactataga taatatagct
     1741 tccagcgcat gtgccataag ggctggaggc ctttttttca tataacaatc gtatagattg
     1801 aagcagcgta ccaggaccct ggtaaatgcc ggagctgcca aactggcacc attggctgtc
     1861 aggtatcgct cgaatcggca tgcccaggct gcaacttcat ggcttcatga ctggcattga
     1921 agccgagcga ctgccagagg gtttgaatac gattcccgca gggcttccaa gcggatcgtc
     1981 ggcgagccac tccaccatgc gctccgattc tgaccggact gatcggcgca aggcgggact
     2041 gggttcgatg cggcctcgat cagcatcgaa tggacttgtc gccacggcgt gtcgtaacca
     2101 atcgtcaccg tggtgtcgag gacatagccc gacctgccgg cactgtttcg gatgcgcgag
     2161 aaattgcgcg tcacattgcc cgccaccaag gcgttcggaa tcgcgatttc ctcgcccatg
     2221 ccggtacgca ggcgggtgac gaaaagactc agttccgtca ccgtgccttc acagtcgttg
     2281 atgcgtacgt acttttgagc tcccatcatc gaccaaaacc attgtcagag gcttatctgc
     2341 gcctggtgac cctcatcgca gagcaagacc agattgactg gggtcacttt ggctaccagc
     2401 aaattggtca ggccaggcgc cccctgatgg cctttgttgc ggagctgtcg tggtcacggc
     2461 gcatcttttt gcgcttctcg cttgatgccc gcatggagag cttcttgcgt gcccatgtcc
     2521 aagcctttga ggtttggggc ggcctgccca gagtgctgct ctatgacaat ctcaaaaatg
     2581 ccgtgcttga gcgtcagggc gatgccattc gtttctcgct cacacggccg gcatttgccg
     2641 ctcactacca cttcgagccc cgctgcggtt acggtggctc ggggcaatga gaaggggcgg
     2701 gttgagcgtg ccattcgcta tatccgtgac aactccttct gcgcgcgcac attcactgac
     2761 cttgatgacc tcaatggccc aggccgatgc ctgggcgtgc ggcccagccg atgagcgttt
     2821 gtgcccgcag gacaagactt tgacggtggg acaggccttt gtccacgaga agccccggct
     2881 gctggcattg ccggccaatc attacccggt gcaagaacgt ttggtggtca aggtcggcaa
     2941 gaccccctat gtgcgctttg acctcaatga ctattcggtg ccgcacaccc aggtgcgcaa
     3001 agcgctcacg gtgttggctg ataccaagac ggtgcgcatc ctcgatgccc aggtgttggt
     3061 ggccagtcat gtgcgcagct acgtaaccta cctgttgagc agatgccatg tggaagattt
     3121 gatgcaagag ccaagcggcg gccagtttga tcatgcgcac ccgcgtcaag gtgtacttct
     3181 ggctgcgccg tcgcaacgac tttagccata tctccaccac gcggtaacga aatgccccaa
     3241 tcgcagggga gttggtcggt aacgcatgat acgcatagaa cccgtgcata accgctgcaa
     3301 acagcaggtt cgcccgactg aaattccgat cctaaaaatt gtttccgcct gttacttttc
     3361 caggccaatc gccgctattg ccaaatcaaa gggaggcaag cgactcgccg ccctcgcggg
     3421 cattactctt tccggattag tgtgtcgccc agcgcgttcg gtcgtttgtc cagggtgcac
     3481 atgttgttgt cggccggcct ggctcccagg aagagcttct ttgcgaccac tttcagcagg
     3541 tcgtattcgc ggttgcactt ggccagcggc gcgacgaagg aacagccggt ttccgagatg
     3601 tcgcgctcga cgcctttttc cagcttgcag ttggcaaagc ccaggttatt gatcaggtcc
     3661 gcattgtcgg tcatcagggt gagcgtcttc ttgtcgaaaa gaaacagtgc ttcgctggca
     3721 cctttgacct ttttcgattt cccggtaacg gaataggtgc ctatcccccg aaatacgaat
     3781 accggcatcg tttttgcctc atcgagatac agggtgtact tgacgttcca gttgttgccg
     3841 tcgaacgaaa attccctgaa cgaaaaggtg ttgtttccgg catcgtcaga tttggcggaa
     3901 atccagtttc catcagcccc cttggcggga attgactttg ccagcgcggg agtgcctagc
     3961 gtggcggcga caacgccgat agagatagcc agagcaagca aggttttttt catttcggtg
     4021 ttccttctgg tagagatgtt gcgtctttag aatcggacaa accatttcgg acatgctgcc
     4081 gatggagttc gtaaggatgc aatggaagat gttaaccggc gaatgggtgc tcctccaaag
     4141 gcacggcaaa gtgatcccag cgcaagggtc aatgccatta agttaaggta agtatccggt
     4201 cttcccgtac ggatgttagc ggcgcaaggc cgagaggaat tgtttgttca acgggtaaag
     4261 ccagacatcc ttgataggga tgagcggttt gtggctgaga gactttttgc cgcgaccgct
     4321 ggtgcgcccc acattgatcc aattggccgc cttatagcaa gtaccccggc ggcgatcaaa
     4381 ttctacaaag gtctccaaca atagggggcg aaatccgtaa cgagtctgcc agtcacgcgg
     4441 caattgctgg cgggcgaaac cggccatgat cagcgcgtcc atgagttata tgcaggtttc
     4501 aatccacgcc cgccattgcc ggcgggcgaa ccggggcttc cccataccgc tgctcgctac
     4561 gatgaattgt ttcaatccac gcccgccatt gctggcgggc gaacccctgg cgatgaccgg
     4621 cgcgcacaac gccaagatgg tttcaatcca cgcccgccat tgctggcggg cgaaccctgc
     4681 ggtgatagga agccacttag cggttgtact gtttcaatcc acgcccgcca ttgctggcgg
     4741 gcgaacggag aggcatcgtg accaagccag cgccccagcc agtttcaatc cacgcccgcc
     4801 attgctggcg ggcgaaccgc atgtaggggc tgaaatgcac ggggttatgg atgtttcaat
     4861 ccacgcccgc cattgctggc gggcgaaccc tgcgcatatt gcgcagcccc gaatgccgta
     4921 gtgtttcaat ccacgcccgc cattgctggc gggcgaaccg atccgccgcg ttactctgcg
     4981 ctgtcgcgac tgccgtttca atccacgccc tccattgctg gcgggcgaac
//
LOCUS       DAOSLL010000337         5019 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14918_length_5019_cov_3.940774, whole genome shotgun sequence.
ACCESSION   DAOSLL010000337 DAOSLL010000000
VERSION     DAOSLL010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14918_length_5019_cov_3.940774"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="PLB25_14080"
     CDS             complement(<1..1410)
                     /locus_tag="PLB25_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016835514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HOE42740.1"
                     /translation="MNLKWEPYFDTGIDIVDQQHRGLVDILNEAAPMLARPGGPDMQG
                     IGAVLEKLFSYAAIHFKTEEGLMELHKLDERYVAHHRAIHTQFVEQIAGMRALLASSS
                     ALEVGPSLLRYMTSWLTIHILDEDRRFARHVALIEGGETPDGAYAIVTKNNRGIDPAR
                     AALEAALGDLYEVLGEHNKTLHQANTRLEQARNELELRVQERTHELSDVLEQMKLTQG
                     QLLQSEKMAAVGQLAAGVAHEINNPIGFVNSNLGSLKNYVTQLLNVISAYEEMDAETR
                     PMEGREQHLAQARAEADLDYLRQDVMILLNESVDGLVRVKKIVQDLKDFSHVDQADWQ
                     DADINAGLESTLNVIAHEIKYRATVVKNFTPLPLLRCLPSQLNQVFMNLLLNATQAFD
                     RQGEIGVSTGFTEDEVWVEISDNGSGMPPDVQKRIFEPFFTTKPVGKGTGLGLSIVFD
                     IVQKKHGGRITVDSAVGQGS"
     gene            complement(1407..2786)
                     /locus_tag="PLB25_14085"
     CDS             complement(1407..2786)
                     /locus_tag="PLB25_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE42741.1"
                     /translation="MRPDDSPQHVPLGAATLADTPPTLLFVDDEPSILSSLQRLFRPK
                     GYKILTAESGDAGLAILDSQAVDLVISDMRMPKMDGAQFLEKVRQKTPETMRLLLTGY
                     ADINATINAINKGEIYRHISKPWDDNDILLIVKKALEHKALRAENQRLLVLTQQQNDE
                     LKDLNSGLEKRVQERTAEIEHVNSFLNLANERLKQNFMVSIKVFSGLIELREGAVAGH
                     ARRVADMARRIARQMALPTAAQQDVFVAALLHDIGKIGFTDELLAKPVSKLVNEELAR
                     YRKHALAGEAALMPLVEMQGVAKIIRAHHERFDGAGFPDGAAGAAIPLGAGILAVAND
                     YDGFQIGTLTERRYTADEARILIKQGSDKRYDPQVVAAFLEVFGLPREETGRYRELLA
                     SELVPGMVSGKDILSRDGTLLLAADYVLDAVIVRQIQTYASREGIHLMVSIRTDKRFK
                     TATQAASQP"
     gene            complement(2834..3238)
                     /locus_tag="PLB25_14090"
     CDS             complement(2834..3238)
                     /locus_tag="PLB25_14090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE42742.1"
                     /translation="MKSGKYKCTLVKHCEPGLPKMRCIAAQLNQVFLNLLVNAANAIG
                     TSGDITITTQSLIPGAIRISISETGCGIPTGNRQRIFDQFFTTKPVGQGTGLGLSIVC
                     GIVRKHYGKIDVESTVGRGSTFVLTLPVDPPA"
     gene            3700..4212
                     /locus_tag="PLB25_14095"
     CDS             3700..4212
                     /locus_tag="PLB25_14095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42743.1"
                     /translation="MSTWLVRIGLRPIAWLLCIVVAVTCALAWVGSVMFANERLDRYM
                     VQEESHARNDALLTSANLNQRLNQAISVARVLALDESMMAALARFGPSIQASTLPQPE
                     RGAVWRADPALKVISERMQRTVEQFDLHSLQLVNAAGDTVAEGHGTGLPPLSAPTMPT
                     ASTSKSPTRG"
     gene            4209..>5019
                     /locus_tag="PLB25_14100"
     CDS             4209..>5019
                     /locus_tag="PLB25_14100"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE42744.1"
                     /translation="MIGKQFLLGRVTNVFALGFSVPVQVKGEFVGMVGSGLVMPKLSP
                     AIENVHAVVTDDLGVIVLAKDPALLMQTMPGATVNKLTAEARNKRYKRTDFEPADISP
                     MLPGGPHTLYQWRKGSRPHVMESHSTEDGSLRVHVLRDLGEPFSNIRSDQVWWFGLIS
                     ILVLLTAALLGVAAKFMISTQTQQAALLHLNEKLACEANTDALTGCANRRNFMHQLEQ
                     EYDRSGRYNFAMCLLSLDIDHFKRVNDTHGHAAGDEVLKHFVAIIQCNLRQV"
BASE COUNT         1042 a         1385 c         1419 g         1173 t
ORIGIN      
        1 gctaccttgc ccgaccgcac tgtcgaccgt gatgcgtcca ccatgtttct tttgtacgat
       61 gtcaaaaacg atggataagc ccaacccggt gcctttgcca acaggcttgg tggtgaaaaa
      121 cggctcgaaa atccgctttt gcacgtcggg tggcattcca ctgccgttgt cactgatttc
      181 aacccacacc tcgtcctccg taaagccagt gctgacccca atttcgccct gtctatcgaa
      241 tgcctgagtc gcgttaagca gcaagttcat gaagacctgg ttgagctggg atggcaggca
      301 gcgcagcaag ggcaatgggg tgaaattctt gacgaccgtg gcccgatatt taatttcgtg
      361 agcgatgaca ttgagtgtgg actccagccc ggcattgatg tcggcgtcct gccagtctgc
      421 ctggtcaaca tgcgaaaaat cctttaagtc ctggacaatt tttttgaccc tcaccagccc
      481 atctaccgat tcgttgagca ggatcatcac atcttgccga aggtagtcca ggtcagcctc
      541 tgcgcgcgcc tgggccagat gttgctcgcg cccctccatg gggcgagttt cggcatccat
      601 ttcttcatac gcagagatga cgtttagcag ctgggtgaca tagtttttga gcgaacccag
      661 gttggaattg acaaagccga tggggttgtt gatctcatgg gccacgccgg ctgccaattg
      721 gccaacggcg gccatttttt cggactgcaa aagctggcct tgggtcagtt tcatctgctc
      781 cagcacgtcg ctcagctcat gggtgcgttc ctgcacccga agttcaagct cgttgcgcgc
      841 ctgttccagt ctggtattgg cctggtgcaa ggttttgttg tgttcaccca gtacctcata
      901 caggtcgcca agggcagctt ccagcgcggc gcgcgcaggg tcgatgccgc ggttgttttt
      961 ggtgacgatg gcataggcgc catcaggcgt ttcgccaccc tcgatcaagg cgacatgtcg
     1021 cgcgaagcgc cggtcttcgt ccagaatgtg aatcgttagc cagctagtca tgtaacgcag
     1081 cagtgacggg ccgacttcga gagcgctgct ggatgccagc aatgcgcgca tgcctgcgat
     1141 ctgctccaca aactgcgtgt gaatggcacg gtggtgcgca acatagcgct catcaagctt
     1201 atgcagctcc atcaaacctt cttcggtttt gaagtggata gcggcgtagc tgaaaagctt
     1261 ttcgagcacg gcgcctatgc cttgcatatc cgggccaccc gggcgggcca gcatcggggc
     1321 ggcctcgtta aggatgtcca ccagcccgcg gtgctgctgg tcaactatat cgatcccggt
     1381 atcaaaatag ggttcccatt tcaggttcat ggctggctcg cagcctgtgt ggccgtttta
     1441 aaccgcttgt ccgttcgaat gctcaccatg aggtgaatgc cctcgcggct ggcgtaggtt
     1501 tggatctggc gcacgatgac ggcatcaagc acatagtctg ccgccagcaa cagggtgccg
     1561 tcgcggctca ggatatcttt gcccgacacc atgccgggca ccagttcgga ggctagcagt
     1621 tctcggtagc gtccggtttc ttcgcggggc aggccaaaga cttccagaaa tgctgccacg
     1681 acctgggggt cgtaccgctt gtcactgcct tgcttgatca agatcctggc ctcatctgcg
     1741 gtgtagcggc gctctgtcag ggtgccaatc tgaaacccgt cgtagtcatt ggcgaccgca
     1801 agaatgccag cgcccagagg aatcgcggca ccggccgcgc cgtccggaaa tcctgcacca
     1861 tcaaatcgct cgtggtgggc gcgaatgatt tttgccacac cttgcatctc aactagtggc
     1921 atcagcgcgg cctcacccgc cagggcgtgc ttgcggtagc gtgcaagttc ttcgttgacc
     1981 agcttggaga ccggcttggc cagcagctca tcggtaaagc cgatcttgcc gatgtcgtgc
     2041 aacagcgctg caacaaacac atcttgttgg gctgctgtgg gcaatgccat ttgcctggca
     2101 atgcggcgtg ccatatcggc tacccgacgg gcatgcccgg ccaccgcgcc ttcgcgcagc
     2161 tcgatcaggc cggaaaagac cttgattgac accataaagt tctgcttgag gcgttcattg
     2221 gccaaattca gaaagctgtt gacatgttca atttctgcgg ttcgctcctg gacccgcttt
     2281 tccagtccgg agttgaggtc cttgagctcg tcgttttgct gttgggtgag aacgagcagg
     2341 cgctgatttt cagcgcgcaa tgccttatgc tccaacgcct ttttgacgat cagcagaatg
     2401 tcgttgtcgt cccacggctt ggaaatatgg cggtaaattt cgcctttgtt gatggcgttg
     2461 atggtggcgt tgatgtcggc atagccagtc aacagcaggc gcatggtctc aggcgttttt
     2521 tggcgaacct tttcaaggaa ctgggcaccg tccatcttgg gcatgcgcat gtcggagatg
     2581 accagatcaa cagcttggct gtcaagaatg gccaaaccgg cgtcaccact ctcggcagtg
     2641 agtatcttgt agcccttggg tcgaaacagt ctttgcagcg aactcaggat cgacggctcg
     2701 tcgtccacaa acagcagtgt gggcggggtg tcagccagtg ttgccgcacc aagtggcaca
     2761 tgttgcggtg agtcgtcggg gcgcatgtat cagtacctgt tatttagggg gtcacttggg
     2821 taaaaaagcg cgttcaagca gggggatcaa cgggcaatgt cagcacgaaa gtggaaccac
     2881 gaccaactgt actctcaacg tcaatctttc cgtagtgttt gcgcactatg ccgcaaacaa
     2941 tggacagacc gagtccggtg ccttgcccga cgggtttggt ggtaaaaaat tggtcgaaga
     3001 tgcgctgccg gttgccagtg ggaataccgc aaccggtttc ggaaatgctg atccggatgg
     3061 caccggggat tagactctgg gtggtgattg tgatgtcgcc gctggtccca atggcgtttg
     3121 ccgcattgac cagcagatta aggaatacct gattgagttg cgcggcgatg caacgcattt
     3181 tcggcagacc gggttcgcaa tgcttgacca gtgtgcactt gtatttgcca gatttcacga
     3241 cttggtcacc agaaagctga ccgtcgcttc gtttcagccg atggccggtt tggaggagcc
     3301 agatcggaaa cggttactac cacttagggt ctgctgaagt caaccgccca aaattgcccc
     3361 gttttcactc gacgggcttc agtaggctga aagcgggtct ccagtttgcc gatctggctg
     3421 ccacgtacct gccgttcaat caagcgactc tgacgggatc aagcaccaac gaagtgcggt
     3481 gaatcacctc tgccatgtgc ggcttttagc aagtgctcct gttcataggc gccttctcgc
     3541 aaaggactca tctatgaagc gttaaccgag agctttctct ctttgccaga ctgtcattgc
     3601 atgtgcgaaa ctcaactaaa tgaaaacgag ctttctgccc ggcgaagttg gccagtcact
     3661 cttctgcgaa cgggacttga tcgagcctcc cactacggca tgagtacctg gttggttcgc
     3721 atcggtctcc gtccaattgc ctggctgctg tgtatcgtgg tagcggtcac ctgtgccctt
     3781 gcttgggtgg gctcagtgat gtttgccaat gagcgccttg atcggtacat ggtgcaagaa
     3841 gaatcacatg ctcgcaacga tgccctgctg accagtgcta acctgaatca acggctaaat
     3901 caggccatca gcgttgctcg ggtactcgca cttgatgagt ccatgatggc tgcgctggca
     3961 cgtttcggac cgagcataca agcatcaacc ctgcctcaac ccgagcgtgg cgcagtctgg
     4021 cgtgccgacc cagcgctcaa agtaatttct gaacgcatgc agcgaacagt cgagcagttt
     4081 gatctccact cactacagct tgtcaatgca gcgggcgata ccgtggcaga agggcatgga
     4141 acaggccttc cccccttatc ggcaccaact atgccgaccg cgagtacttc aaagtcgccc
     4201 acaaggggtt gatcggaaag caattcctcc tcggccgggt caccaatgtg ttcgctttgg
     4261 gtttctcagt tccagtgcaa gttaaaggcg aatttgtggg aatggtaggt tccggattag
     4321 tcatgccaaa acttagtccc gctattgaga acgtacatgc ggtggtcacc gatgacctag
     4381 gtgtgatcgt gttagccaaa gaccctgcac tgctgatgca aaccatgccc ggtgccaccg
     4441 tgaacaagct taccgccgag gcgcggaaca aacggtacaa gcggacagac tttgagcctg
     4501 ccgatatcag cccgatgttg ccaggtgggc cgcacacgct ttaccagtgg cgcaagggat
     4561 cgagacctca cgtgatggaa agccactcga cagaagacgg atcccttcgc gtccatgtgc
     4621 tgcgcgacct tggagaaccg ttttccaata tccgatctga ccaagtgtgg tggttcggac
     4681 tgatcagcat cctggtgctc ttgactgcgg cattgctggg tgttgcggcg aaattcatga
     4741 tcagcacaca aacgcagcag gctgcgctgc tgcacttgaa cgagaaactg gcctgcgagg
     4801 ccaataccga tgcgctgacc gggtgcgcca accgacgcaa cttcatgcac cagttggagc
     4861 aagaatatga tcgttcaggc cgttacaact ttgccatgtg cttgctcagc ttggacattg
     4921 accactttaa gcgtgtcaac gacacccatg gccacgccgc cggagacgaa gtgctgaagc
     4981 attttgtggc aatcattcag tgtaatttgc gccaggttg
//
LOCUS       DAOSLL010000338         5010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14953_length_5010_cov_4.202422, whole genome shotgun sequence.
ACCESSION   DAOSLL010000338 DAOSLL010000000
VERSION     DAOSLL010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5010
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14953_length_5010_cov_4.202422"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2336
                     /locus_tag="PLB25_14105"
     CDS             <1..2336
                     /locus_tag="PLB25_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOE42745.1"
                     /translation="IPLPRMAEYTDGIERINIELSLTNKLALCDSLQDFFTQGHLPLG
                     RTDDASEISAAELLEDRVTQALALLAEVRALWAGWLQQIAPLFTQLQDHSLRASWKTQ
                     LRQPLQQIFSGLAFEPILAQCNAIHQRVLKGRVWVALHMHAGDGNVHTNIPVNSDDYA
                     MLQTAHAAVKRIMALARSLGGVISGEHGIGITKLEFLTDAELQPFTDYKARIDPQGHF
                     NKGKLLRNKELPAQIQQGPAADLPIMPDDNRKLLLNSELLTQVQQGLEADLTLFSDLS
                     NAYTPSFGLMGHESLIMQQSAIGAIAVSVKDCLRCGKCKPVCSTHVPRANLLYSPRNK
                     ILATSLLVEAFLYEEQTRRGVSIKHWQEFEDVADHCTVCHKCLSPCPVNIDFGEVSMN
                     MRHLLRKMGKKSFRPAGAAAMFMLNVTNPKTLKAARSVMVNLAIPAQRLAAGLLRVVA
                     RHQTQAPPATLGTAPIREQVIHFINKKLPGGLPKKTARALLDLEDKQYVPIIRNPKTT
                     TAETEAVFYFPGCGSERLFSQVGLATQAMLWHAGVQTVPPPGYLCCGYPQRGGGQYER
                     AEKMITDNRVLFHRVANTLNYLDIKTVVVSCGTCFDQLQGYQFDKIFPGCRIIDIHEY
                     LLEKGITLAAAGAFLFHDPCHSPMKLQEPLKTVKALLGQQVVQSERCCGEAGTLALNR
                     PDIATQVRFRKEEEIRKGEAQLRQLGGVAASDNLKILTSCPACLQGLSRYGDDLQAGL
                     LQADYIVVEMANQILGQQWLPDYVKLATEGGIERVLV"
     gene            2410..2796
                     /locus_tag="PLB25_14110"
     CDS             2410..2796
                     /locus_tag="PLB25_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HOE42746.1"
                     /translation="MPQDMTVHSQSRVLEVSFSDGATFRIPFELMRVRSPSAEVQGHG
                     PGQETVQSGKRQVDLIALEPVGNYAVQPTFSDGHSTGIFTWDYLYFLGSQQEQLWQEY
                     EAQLQAAGVDRDTPMAGKAAGSCATH"
     gene            2808..3539
                     /gene="ubiE"
                     /locus_tag="PLB25_14115"
     CDS             2808..3539
                     /gene="ubiE"
                     /locus_tag="PLB25_14115"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005797375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HOE42747.1"
                     /translation="MTSTHFGYKSVDESEKAKHVKGVFDSVAPKYDLMNDLMSMGLHR
                     LWKAYTVTVANLHEGDQVLDIAGGTGDLALAFSRKVGRSGRVVHTDINEAMLRQGRDR
                     LVDAGIILPTLVCDAEKLPFQSQHFNLVSVAFGLRNMTHKELALAEMNRVLKPGGKLL
                     VLEFSKVAKPLEKAYDWYSFKVLPRLGQLVAGDASSYQYLAESIRMHPDQESLKTLMQ
                     ANGFGHVDYHNLTGGLVALHVGIKC"
     gene            3587..4572
                     /locus_tag="PLB25_14120"
                     /pseudo
     CDS             3587..4572
                     /locus_tag="PLB25_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073948.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44-like domain-containing protein"
     gene            4609..>5010
                     /locus_tag="PLB25_14125"
     CDS             4609..>5010
                     /locus_tag="PLB25_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42748.1"
                     /translation="MATQSPFSFLEGFLQKLPLPKLQPPTWAVTEAQRRIVLLLNHVL
                     MQESEAMARLARHKGQVMLVQWRSFSFRLQSTPAGLLDLAAPQSPADLVLTVTDESPL
                     ALLQHAFRGDKPGVRIEGDVQLAAEVNWLTEH"
BASE COUNT         1063 a         1435 c         1507 g         1005 t
ORIGIN      
        1 tgatcccgct gccgcgcatg gccgagtaca ccgacggcat cgagcgcatc aacattgaac
       61 tcagcctgac caacaagctg gcgctgtgcg attcgttgca ggactttttc acccagggtc
      121 atttgccgct aggtcgcact gacgatgcca gcgagatttc agccgccgag ctgctggaag
      181 accgggtgac gcaagccctg gcgctgctgg ccgaggtgcg cgccttgtgg gctggctggc
      241 tgcaacagat tgcgccactg ttcacccaat tgcaggatca cagcctgcgc gccagttgga
      301 aaacccagct tcgccagccc ttgcagcaga ttttttccgg gctggcgttc gagccgattc
      361 tggcccaatg caatgcgatt caccaacggg tgctcaaagg cagggtctgg gtcgccctgc
      421 acatgcatgc cggtgatggc aatgtgcata ccaacatacc ggtcaacagc gatgactatg
      481 cgatgctgca aaccgcccac gctgcggtca agcgcatcat ggcgttggcg cgttcgctgg
      541 gcggggtaat ttcgggcgaa cacggcattg gcatcaccaa gctggagttt ttgaccgatg
      601 cagagctgca gccgttcacc gattacaagg caagaataga cccgcaaggg catttcaaca
      661 aaggcaagct gctacgaaat aaagagctgc ccgcgcagat acagcaaggg ccagcggctg
      721 atttgccaat aatgcctgac gacaatcgga aattgctact aaatagtgag ctgcttacgc
      781 aggtacagca agggctagag gccgatttga ccctgttttc tgacctgagc aacgcctaca
      841 ccccgagttt tggcctgatg gggcacgagt cgctcatcat gcagcagtcg gccattggtg
      901 cgattgccgt cagcgtgaaa gactgcctgc gctgcggcaa gtgcaagccg gtgtgttcga
      961 cccatgtgcc gcgtgccaat ctgctgtatt ccccgcgcaa caagattctg gcaacttcac
     1021 tgctggtcga agcctttttg tacgaggagc agacccgccg gggtgtcagc atcaagcatt
     1081 ggcaggagtt tgaagacgtg gccgaccact gcacggtgtg ccacaaatgc ctgtcgccct
     1141 gccctgtcaa tatcgacttt ggcgaggtgt cgatgaacat gcgccacctg ctgcgcaaga
     1201 tgggcaaaaa gagctttcgc ccggccggtg ccgccgccat gttcatgctc aatgtcacca
     1261 accccaagac cctcaaggcg gcccgatcgg tgatggtcaa tctggccatc ccggcgcagc
     1321 ggctggcggc cgggctgctg cgggtggtgg cacggcacca gacccaggca ccaccggcca
     1381 cgctgggcac cgcacccatc agggaacagg tgatccactt catcaacaag aagctgcctg
     1441 gtggcttgcc caagaaaacc gcgcgcgcct tgctggatct ggaagacaag caatacgtgc
     1501 cgatcatccg caacccgaaa accacgacgg ccgagactga agcggtgttt tactttcctg
     1561 gctgcggctc cgagcggctg ttcagccagg tcggcctggc cacgcaggcc atgctgtggc
     1621 acgccggggt gcaaaccgtg ccgccgccgg gctatctgtg ttgcggttac ccgcagcgcg
     1681 gtggtggcca gtatgaacgc gccgagaaaa tgatcaccga caaccgggtg ctgtttcacc
     1741 gggtcgccaa cacactgaac tacctggaca tcaagaccgt ggtggtgagc tgcggcacct
     1801 gttttgacca gttacagggt taccagttcg acaagatctt tccgggctgc cgcatcatcg
     1861 acatccatga atacctgctt gaaaaaggca tcaccctggc cgctgctggc gcgtttctgt
     1921 ttcacgaccc ctgccacagc ccaatgaaat tgcaggaacc gctcaagacc gtgaaagccc
     1981 tgcttggtca acaggtggtg caatcagagc gctgctgcgg cgaagccggc acgctggcct
     2041 tgaaccggcc cgacattgca acccaggtgc gttttcgcaa ggaagaagaa attcgcaagg
     2101 gagaggcgca gttgcgtcag cttggcggcg tggcggccag cgacaatctg aaaattctca
     2161 cctcgtgccc ggcctgtctg caaggcctga gccgctatgg tgatgatttg caagccgggt
     2221 tgctgcaagc tgattacatt gtggttgaaa tggccaatca gatactgggc cagcaatggc
     2281 tgccggatta cgtcaaactc gccaccgaag gcggcatcga gcgggtgctg gtctgaccgg
     2341 tttttgcaga aatatgattg ctttgggaac aggagattta ccttgacaga ttcacaaacc
     2401 atagcgccga tgccgcagga catgactgtg cacagccagt cgcgcgtgct ggaagtcagc
     2461 ttttcagatg gcgcaacttt tcgcattccg ttcgagttga tgcgggtgcg ctcaccgtct
     2521 gccgaggtac aaggccatgg gccgggacag gaaaccgtgc aaagtggcaa gcgccaagtt
     2581 gacctgattg cactcgagcc ggtcggcaat tacgcagtcc agccgacctt ctcggacggt
     2641 cacagcaccg gcatcttcac ctgggactac ctgtattttc tcggttcgca gcaagaacaa
     2701 ctgtggcaag agtacgaggc ccaattgcag gctgctggcg tagaccgcga cacgccgatg
     2761 gccggcaaag ccgctggcag ttgcgcaacc cattgaaaag cacaaagatg accagcacac
     2821 atttcggcta caagagcgtc gatgagagcg aaaaggccaa acacgtgaaa ggcgtgtttg
     2881 actcggtggc acccaaatac gacctgatga acgacttgat gtcgatgggc ttgcaccgtc
     2941 tgtggaaagc ctacacggtg acagtggcca atctgcatga aggcgaccag gtgctcgata
     3001 ttgcaggtgg caccggtgat ctggcgctgg cgttttccag gaaggtggga cgcagtggtc
     3061 gggtggtgca cactgatatc aacgaggcca tgctgcgcca ggggcgtgac cggctggtcg
     3121 atgccgggat catactgccg acgctggtgt gtgatgccga aaagctgcca tttcaaagcc
     3181 agcacttcaa cctggtgagc gtcgcgtttg gcctgcgcaa catgacacac aaggagctgg
     3241 cgttagccga gatgaaccgg gtgctcaagc ccggtggcaa gttgttggtg ctggagttct
     3301 ccaaggtggc caaacctttg gagaaagcct atgactggta ttccttcaag gtgctgccac
     3361 gcctgggcca actggtggca ggtgatgcgt caagctacca atacctcgcc gagtccatcc
     3421 gcatgcaccc tgatcaggaa tcgctcaaaa ccctgatgca ggctaatggc tttggccatg
     3481 tcgattacca caacctgacc ggtggtctgg tagccttgca cgtgggtatc aaatgctgag
     3541 tgaagtcggc ttcgagcttt tatttaaaac accaattgga gaacacatga aacgatggct
     3601 atgggtgatc atgatgagcc tggctttgtt tgccggccac gctgatgcgg caaagcgctt
     3661 tggcggcggc aaatctatcg ggcaacagtc caccaatgtg acgcagcgtc aggcagcgcc
     3721 tgcacccgca acgccacagc aaggtgccac caatgccgct acacccggtg ctgcggcggc
     3781 cgcagctact cccaaaaagc catggggcgc gatgctcggc ggtttggcgg ccgggctcgg
     3841 tctggcctgg ttggccagtt cgctcggttt gggcgaagca atgggccaat tcctgatgtt
     3901 tgccctgctg gctttggtga tcatgatggc agtcggctgg ttcatccgtt cccgcaaggc
     3961 aaagggtgcc gccgcctcgg catcgccttt tgcgttcaag gtgcaggagc cgccactcag
     4021 ccttcaacgc ctgcagacta cagtccggca agtgtcggca acgacgcttc ggcccgtccg
     4081 tgggagcgct ctggcacagc gtttgaaagt gcccacatgg caggtggtgt tgcggctggc
     4141 ggctccatga tcggttcgag cctgggcggt tcgcaagcct ggggaattcc gcccggattt
     4201 gattcggaag ggtttctgaa gtctgccaaa gccaattttg tgtcgctgca ggcagcctgg
     4261 gacaaggctg acattgccgc cttgcgtgcc atgatgactg acaccatgct caaggaaatc
     4321 caggcgcagt tggctgatcg cgaagcccac accggtggcc cgctgaacct gaccgacgtg
     4381 gtggtgatcg acgcaagctt gctaggtatt gaagacctgg gcagcgatta cctcgccagc
     4441 gttgaatttt ccggcatgat ccgtgaagag ccgtcggctg gcccaagccc gttccgggag
     4501 gtctggaaca tgatcaagcc caaggatggt cacagcggct ggctggtcgc cggggttcaa
     4561 gcgctgcagt aagcgttgcg tttcaaattc agggaataat cggggactat ggcaacacag
     4621 tccccttttt catttctgga aggctttctt cagaaacttc cgcttcccaa actacagccc
     4681 ccaacttggg cggtcactga ggcgcaaagg cgtatcgtcc tgctgctcaa ccatgtgttg
     4741 atgcaggaaa gcgaggccat ggccagactg gcacgacaca agggtcaggt catgctggtg
     4801 caatggcgct cgttttcgtt cagactgcag tcaacaccgg cgggtctgct cgatctggca
     4861 gcaccgcaat ctccggcaga cctggtgctg accgtcaccg acgaatcgcc cctggctttg
     4921 ttgcaacacg cctttcgcgg cgacaagccg ggcgtgcgga ttgaaggtga tgtccaactg
     4981 gcggccgaag tcaactggct gaccgagcat
//
LOCUS       DAOSLL010000339         5009 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14955_length_5009_cov_4.523617, whole genome shotgun sequence.
ACCESSION   DAOSLL010000339 DAOSLL010000000
VERSION     DAOSLL010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5009)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5009)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5009
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14955_length_5009_cov_4.523617"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            97..984
                     /gene="yqeB"
                     /locus_tag="PLB25_14130"
     CDS             97..984
                     /gene="yqeB"
                     /locus_tag="PLB25_14130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HOE42749.1"
                     /translation="MEQQNDLFALARVVKNQGSRPRHWGELVVIRGAGDIASGVAIRL
                     ARCGYRVVMLEIPLPTAIRRSAAFAQAVFDGSVTVEDLTARLVATAHEALDLTLRREI
                     ALLVDPHAGSLGALGPDYLVDAILAKENLGTSKNMAPITIALGPGFTVGDDCHAVIET
                     NRGHRLGRVILSGGAEPNTGIPGEIAGKSDLRVVRAPCAGVFRSYVALGDLITQGTLM
                     GQVGNTAVTAPLTGMVRGLLQDGLQVSARFKIGDIDPRGEKADYLSASDKAMAIAGGV
                     LEAMLRLQIAAMTSNAQLT"
     gene            complement(972..2759)
                     /locus_tag="PLB25_14135"
     CDS             complement(972..2759)
                     /locus_tag="PLB25_14135"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HOE42750.1"
                     /translation="MPKTPVTHPSVLQAIQPTIVRFTKMLAGLLRLEVEIVDANRIRV
                     AGTGPYASMTGQRLHTDACFLRQVLDTKQEKVVLQPRAEPLCTGCADLLPGNTCAFVG
                     IPIVAEQQCVGVINLIALTAESQLRIREHSGEIIEYVRHVSSLFVSKIIGDHDGSRDH
                     DKQLLSLLDHMDQGVVLIDEDQRVCLINPPALAQLNCNQENIIGRALSIRPCVDHQEV
                     PSGHLQYMVSFGDHHEVIVGQLHKVHDRQLFLMAFHQAHPPLSNQLVRNEPRLSHIIG
                     ESPPMRELKALLIRVATSPSSILICSESGTGKEIVARAIHQLSGRAKRPFVAINCAAI
                     PDGLQESELFGYTKGSFTNASTTGKSGLIQSADGGTLFLDEIGDMSLSTQAKLLRVIE
                     TREVQPLGANKAISVDIRIVAATHQNLQQRIAEGKFREDLYYRINVIPLAIPPLRDRG
                     GDIELLVHHYINQHSRRLGAVYPGISPAVLKLLPKYSWPGNVRELSNLMEYLVNVVPN
                     GEVIDERLFPPNITKGLLSSGLDAPVQTMEVAQPQTRPDATLIESMERQLIEASLLRH
                     KNKQQAADELGIGIATLYRKVKKYGLQVS"
     gene            3113..4495
                     /locus_tag="PLB25_14140"
     CDS             3113..4495
                     /locus_tag="PLB25_14140"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HOE42751.1"
                     /translation="MNVDFGAIKAAAEGYKADMSRFLRDMIAIPSESCDEEKVVKRIA
                     AEMEKLGYDKVEIDGLGNVIGWMGEGDKIIAIDSHIDTVGIGNINNWTNDPYTGFETD
                     EVIYGRGGSDQEGGMASATYGVKIMKDLGIIPAGYKIMVVGTVQEEDCDGMCWQYIYN
                     VSKIVPEFVISTEPTDGGIYRGHRGRMEIRVDVKGVSCHGSAPERGDNAIHKMADILQ
                     DIRSLNENDAAQGTAIKGLVKMLDPQYNPRHYEDARFLGRGTCTTSEIFFTSPSRCAV
                     ADSCSISVDRRMTAGETNLSCLKEIENLPNVKKYGADVKVSMYWYDKPSWTGEVYKTE
                     CFFPTWINKESAAHVQALVEAHHAMWGDTRLSPPDEIALSKRVGRPLTDKWTFSTNGV
                     SIQGRYGIPCVGFGPGAESQAHAPNEITWKRDLVVCAAMYAAAPALYKPENKVTEAEF
                     RQELTGNLIK"
     assembly_gap    4645..4654
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
BASE COUNT         1084 a         1367 c         1455 g         1093 t
ORIGIN      
        1 ccggctcagg tgctggctca gttgctcacc gatgcgcagc ctcgggtcag gtagccacgt
       61 catgaagtca tgggcatttt ttctggaact gcgcgcttgg aacaacaaaa cgacctgttt
      121 gccttggcgc gcgttgtgaa aaatcaaggc tcaagaccac ggcattgggg tgagttggtt
      181 gtgattcggg gtgccggtga cattgcctcc ggcgtggcga ttcgactggc gcgctgcggc
      241 taccgcgttg tcatgctgga gatccccttg cccaccgcga tcaggcgcag cgcggctttc
      301 gcacaggctg tattcgacgg ctctgtcaca gttgaagacc tcaccgccag gttggtagcg
      361 acggcgcatg aggcgctgga cctgacgttg cgccgggaaa tcgcgttgct ggtggatccc
      421 catgccggtt cactgggtgc gttagggcca gactatctgg ttgacgcgat tttggccaag
      481 gaaaatcttg gcacaagcaa gaatatggca cccatcacga ttgcgctcgg gcccggattc
      541 accgtcggcg atgattgtca tgcggtgatt gaaaccaacc ggggccaccg attggggcgc
      601 gtcattctca gcggcggggc cgagcctaac accggcattc cgggcgaaat cgccgggaaa
      661 agcgatttgc gggttgtgcg agcgccatgt gctggcgttt tccgctcata tgttgcgctc
      721 ggtgacctca tcacccaagg cacactgatg ggtcaggttg gcaacacggc ggtgaccgcg
      781 ccgctgaccg gcatggtgcg cggccttctg caagacgggc tgcaggtcag cgccaggttc
      841 aagattggcg acatcgatcc gcgcggtgaa aaggccgact acctcagcgc atccgacaag
      901 gccatggcca tcgctggtgg cgtgctggag gcaatgcttc gccttcagat tgcggcaatg
      961 acatcaaacg ctcagctcac ctgaagcccg tatttcttga ctttgcggta cagcgtggca
     1021 atgccgatgc ccagttcgtc ggcggcctgt tgcttgttct tgtggcgtag cagcgaggct
     1081 tcaatcaact ggcgttccat gctctcgatc aaggtggcat caggccgagt ttggggttgc
     1141 gcgacttcca tggtttgcac gggagcatcg agcccactgg acagaagtcc tttggtaatg
     1201 ttgggcggaa acaagcgctc gtcgatcacc tcgccgttgg gcacaacatt gaccagatat
     1261 tccatcaaat tgctgagctc gcgcacattg ccaggccagg aatacttggg cagcagtttc
     1321 agcacggccg gcgaaatgcc tggatagacc gcacccaagc ggcgcgaatg ctggttgatg
     1381 tagtgatgaa ccagcaattc gatgtcacca cctcggtcgc gcaagggtgg aatggccagc
     1441 ggaataacgt tgatgcggta atacaggtct tcacgaaatt tgccctcggc aatgcgctgc
     1501 tgcagatttt gatgcgttgc cgcgacgatg cgaatatcca ccgatatggc cttgttggcc
     1561 ccgagtggtt gtacctcgcg cgtctcgatc acccgcagca gtttggcctg cgtgcttagc
     1621 gacatgtcgc cgatctcgtc caaaaaaagc gtgccaccgt cggctgactg gatgagacca
     1681 cttttcccag tcgtcgaagc gttggtgaat gagcctttgg tatagccaaa caactcactt
     1741 tcctgcagac catccggaat cgctgcacag ttgatggcaa caaaaggccg tttggcgcgc
     1801 ccgctgagct ggtgaatcgc acgcgcaacg atttccttgc cggtgccgct ctcgctgcag
     1861 atcagaatgc ttgacgggct ggtcgccacg cggatcagca aggctttgag ctcccgcatc
     1921 ggcggtgatt cgccaatgat gtgactcagg cgtggctcgt ttcgcaccaa ctggtttgac
     1981 aggggaggat gcgcctggtg aaacgccatc aggaacaatt ggcggtcgtg caccttgtgc
     2041 aactgtccaa cgatgacttc atggtgatca ccgaaggaca ccatatattg cagatgccct
     2101 gatgggacct cctggtgatc aacacaagga cgaatgctca gtgcgcgacc aatgatgttt
     2161 tcctgattgc agttgagttg cgccagcgcc ggaggattga tcaggcaaac ccgttgatcc
     2221 tcatcgatca gcaccacccc ctgatccatg tggtccagca agctgagtaa ctgcttgtca
     2281 tgatctctgc tgccgtcgtg atcgccaatg attttcgaga caaacagact cgaaacatgc
     2341 cgaacgtact cgatgatttc gccagaatgc tcgcgaatgc gaagctggct ctccgctgtc
     2401 aaggcgatca aattgatgac accgacacac tgttgctcag ccacgatagg aatcccgaca
     2461 aaggcgcacg tgttaccggg caatagatcg gcgcaaccgg tgcacagggg ctctgcgcgg
     2521 ggctgcaaaa cgaccttctc ttgcttggtg tcgagcacct ggcgcaagaa acaggcatcg
     2581 gtgtgcaatc gttggccggt catggacgcg taagggccgg tgccggcaac ccgaatccgg
     2641 ttggcatcga cgatttcaac ctccagacgc agcaagccgg ccagcatctt cgtgaagcgc
     2701 acgatcgtcg gctgaatggc ctgcaacaca gatgggtggg taaccggggt tttaggcata
     2761 tcgttagcgg taaaggctta tcaaactgat aatttgtggc tggaatcgta tcaccacaga
     2821 ccagtcgaag ctggtccact ggggctgcgg cagccttcca gcctcctgca cgactggtcc
     2881 gatacgctgc gggcaaaaaa ggacccatcg gggctggcac gattattgaa tatggtgatg
     2941 aaatgcaagt gcgcgacatt aggcggatgt gcgttgactg tttcttgact gacttgtttt
     3001 gaggtctatc aatgaaggca ttcgaatctc ttcgtttcca tctcatcgat ttcaattgac
     3061 cgcttgaaac aggttggaga tggtatgtat tcaatttaac tgaggtaaat ccatgaacgt
     3121 tgattttggt gccatcaagg ctgccgctga aggctacaag gcagacatgt ccaggttcct
     3181 gcgcgacatg attgcaattc cgtccgagtc ctgcgacgaa gaaaaagtgg tcaagagaat
     3241 cgccgctgaa atggaaaagc tgggctacga caaggttgaa atcgacggcc tgggcaacgt
     3301 cattggctgg atgggcgaag gcgacaagat catcgccatc gactcccaca tcgacaccgt
     3361 cggcatcggc aacatcaaca actggaccaa cgatccgtac accggctttg agacagacga
     3421 agtcatttat ggtcgcggtg ggtcagacca ggaaggcggc atggcttctg ccacttacgg
     3481 tgtcaagatc atgaaggacc tgggcatcat tccggctggc tacaagatca tggtcgtcgg
     3541 cacggttcag gaagaagact gtgacggcat gtgctggcag tacatctaca acgtgagcaa
     3601 aatagttcct gaatttgtca tctccaccga gcccaccgac ggtggcattt accgcggtca
     3661 tcgtggccgc atggaaatcc gggttgatgt gaagggcgtt tcctgccatg gttccgcacc
     3721 agagcgtggt gacaacgcca tccacaagat ggcagatatc ctgcaggaca tccgttcgtt
     3781 gaatgaaaac gatgcggcgc agggcacggc aatcaagggc ctcgtcaaga tgctcgaccc
     3841 gcagtacaac cccaggcatt acgaagacgc gcgcttcctt ggtcgcggca cctgcaccac
     3901 ttccgagatc tttttcacct cgccttcacg ctgcgctgtc gcggactcct gttccatctc
     3961 ggttgaccgt cgcatgactg ctggcgaaac caatctgtct tgcctgaagg aaatcgaaaa
     4021 cctgcccaac gtcaagaagt acggagccga tgtcaaggtg tcgatgtact ggtatgacaa
     4081 gccgtcatgg actggcgaag tctataagac ggaatgtttt ttcccgacat ggatcaacaa
     4141 ggaatccgcc gctcacgtgc aggcactggt tgaagcccac cacgccatgt ggggcgacac
     4201 gcgactgtct ccgccagacg aaatcgccct gtcaaagcgc gtcggccgtc ctttgactga
     4261 caagtggacc ttttccacca acggtgtttc catccagggt cgatatggca tcccctgcgt
     4321 tggcttcggc cccggcgccg agtcccaggc gcacgcgccc aacgaaatca cctggaagcg
     4381 tgacctggtt gtgtgtgccg cgatgtacgc cgcagcgcca gctttgtaca agcctgagaa
     4441 caaggtcact gaagccgaat tccgccagga actcactggc aacctgatca agtaatgctt
     4501 gcttgacgtg aaggtttgaa tagcgttgga gatcagatga aattctgatc cccgtgcttt
     4561 ttgcgcgttg gtggtttgtg atcgtgaaac agcgttggtg aatttgccgt agccgcagtc
     4621 ggtggcaaat tgggaatccg gtccnnnnnn nnnnccggtc cgacccaaaa ggcaccgctg
     4681 cacaggtgcc tccgttgcag gcgcggtcgg tggcaaattt ggaatccgat ccaccccaaa
     4741 gtcaccgctg cattgatgac tctggcgcag gcgccgtcag tagcaaattt ggggtcggat
     4801 cccaattcgg cgaagagttg cctgtttggg tcagcagcgt caccccgaac tggggtctga
     4861 ccccaatttt gcgggccatc gtgcagacct caaaccaatc ggcttgggtc tgcggttgat
     4921 acaacaaatt ggcctccagc ccttatgaat aaagcgctgg cagctctcaa tttggaagct
     4981 gttggtgctg tgccgtgata ttggggtct
//
LOCUS       DAOSLL010000340         5002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_14984_length_5002_cov_3.436224, whole genome shotgun sequence.
ACCESSION   DAOSLL010000340 DAOSLL010000000
VERSION     DAOSLL010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14984_length_5002_cov_3.436224"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLB25_14145"
     CDS             complement(<1..296)
                     /locus_tag="PLB25_14145"
                     /inference="COORDINATES: protein motif:HMM:NF014808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HOE42752.1"
                     /translation="MNFELETSAQTDFLREKSDALLVFLTSAFIQQGDALSTLAAKVL
                     KSGDLLAKPGQLLDIYRPPGFSCSRLVLVHLGDGTVSDVRKGVGAAVALLKASKP"
     gene            321..1409
                     /gene="lptF"
                     /locus_tag="PLB25_14150"
     CDS             321..1409
                     /gene="lptF"
                     /locus_tag="PLB25_14150"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HOE42753.1"
                     /translation="MLFQSSLRKELSRSFGATLIVLVTVVMTMVLIRTLGQASRGSFN
                     PSDVTMVMGYTVLAYMPTLLTMSLFIAIIATLSRMYRDSEMVIWLCSGKGLASLLRPL
                     MRFSWPVFCVVAALSLLVLPWSKQRVDFLKNQYEQRNDIERIEPGRFQESANGNRVFF
                     VEKNVSADQTGSNVFIATNEKGKQTMTSARSGRIEMLAQGQFLLLNNGQRLETVNGAA
                     NLKISEFEVYGVQINTDVTSKYRSAPVNTQTTLSLLQNPTASNLAELSWRFGLILAAV
                     NFVIIGLAASGVNPRANKSVNLVFSLFTFVVYYNLLNLGQSWIASEKLGFATHLLALH
                     GGIFLMATLWLAKCHNNWSWPRLKKAAS"
     gene            1406..2512
                     /gene="lptG"
                     /locus_tag="PLB25_14155"
     CDS             1406..2512
                     /gene="lptG"
                     /locus_tag="PLB25_14155"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HOE42754.1"
                     /translation="MKTLRRLIYAEVINAVAFVALGFLSLFFFFDFVEEVQSIGRQAL
                     PGYQLLQALSYVTLLLPSHLYELMPIAVLIGTIFVMARLAQSSEYTILRTSGLDPWRA
                     LRTLLVLGAGFVIVTFAIGDYLSPVADRTAQFLKARYTGHITLGQTGAWLKEKQDAGQ
                     YAVNVGELAPDGSLRGVRIFEFNNRGTLMSMSLAESAVIEEDDAWLLKQAHQTKFAVH
                     GDSSAQIERVLLPSLRWPNRISAEMVSAALLKPDRMSTIDLFQYIGHLQNNAQTAQRY
                     EIEFWKKVFYPLSCLVMVVLALPFAYLNFRSGGIAAYVFGGVMAGISFFLLNNVFGYM
                     GDLRNWQPWLTAALPGLIYSALSLTAFGWLVLRR"
     gene            2545..2922
                     /locus_tag="PLB25_14160"
     CDS             2545..2922
                     /locus_tag="PLB25_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbiX/SirB N-terminal domain-containing protein"
                     /protein_id="HOE42755.1"
                     /translation="MQATVLFGHGSSDPAWRIPIDQVAQTMLLADPQALVCCAFLERT
                     KPDLASTVADLAQKGVTHVTIVPMFLGIGRHAREDMPMLVKSLQAVYPKLNFRLKPSV
                     GEEPEVIALLARIARPTTNPFAA"
     gene            2925..3878
                     /locus_tag="PLB25_14165"
     CDS             2925..3878
                     /locus_tag="PLB25_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HOE42756.1"
                     /translation="MNLHQFRFVQEAARRDLNLTEAAKALHTSQPGVSKAIIELEDEL
                     GIEIFARYGKRLKRVTEPGQLVLKSIDLIMREVNNLKRVGEHYSLGDSGTLSIATTHT
                     QARYFLPEPVAKLRQTYPKVNIALHQGSPDEVARMVLEEVAEVGIATESLAKYDDLVT
                     LPCYEWHHVLVLPTGHPLASKDHLTLEDIAKEPIITYHPAYTGRTKIDQAFGVRKLTP
                     RFALEAIDSDVIKTYVRLGLGVGIAAEMAVRDIDNDGGKSGLLVRSVGQLFGTNVARV
                     AFKRGAYLRNFVYQFAELLSHRLDRNLVARAMTGQVNDYEL"
     gene            3908..>5002
                     /locus_tag="PLB25_14170"
     CDS             3908..>5002
                     /locus_tag="PLB25_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HOE42757.1"
                     /translation="MSCPRLQSKLPRVGTTIFTVMSALASETGAVNLGQGFPDFACDP
                     RLVDAVTGAMQKGLNQYPPMAGVPALRDTVAMKIEAMYARQYCAATEITITAGATQAI
                     LTIILAIVHNGDEVIVLEPCYDSYCPNIELAGGVPVRVPLTPGTFRPDFEKIAAAITP
                     RTRAIIVNSPHNPSGTVWSHDDMQHLEKLLAATNVMLISDEVYEHMVFDGQQHQSAAR
                     FAGLAERAFVVSSFGKTYHVTGWKVGYVAAPAELTHEFRKVHQFNVFTVNTPVQHALA
                     QYMADPKPYLELPDFYQQKRDLFRAGLEHTRFKLLPCEGTYFQCVDTSSINTLDEARF
                     CKWLTSEVGVAAIPLSAFYANSFDQHVVRFC"
BASE COUNT         1054 a         1413 c         1375 g         1160 t
ORIGIN      
        1 ggtttgctgg ctttgagcaa cgccacggca gcaccaacac ccttgcgaac atcgctgacc
       61 gtgccatcgc ccagatgcac cagcaccagc ctggagcagg aaaagccggg gggacggtag
      121 atgtcgagca actggccggg cttggctagc aggtcaccag acttcaggac ttttgccgcc
      181 agcgtagaca aggcgtcgcc ctgctggatg aatgcacttg tcaaaaaaac cagcaaggca
      241 tccgatttct cgcgcaaaaa gtccgtctgt gccgaagttt caagttcaaa gttcataatt
      301 ggtggtttcc tgtttgcacc atgttattcc aatcctcttt acgcaaagaa ctctcgcgca
      361 gttttggtgc cacactgatt gtgctggtta cggtcgtcat gacgatggtg ctgatacgca
      421 cactgggtca ggcatcgcgc ggcagcttca acccgtcaga tgtcacgatg gtcatgggct
      481 acaccgtgct ggcttacatg cccacgctgc tgaccatgag cctttttatt gccattattg
      541 cgaccttgtc caggatgtac cgtgacagtg aaatggtgat ctggctgtgc agcggcaaag
      601 ggttggcgag cttgctacgg cccttgatgc ggttttcgtg gccggtattt tgtgttgtgg
      661 cagcgctttc actgctggta cttccctgga gcaaacagcg cgtcgatttc ctgaaaaacc
      721 aatacgagca gcgtaacgat atcgaacgga ttgaaccggg tcgttttcag gaatcggcca
      781 acggcaaccg cgtttttttt gtcgaaaaga acgtctcggc agatcaaacc gggtccaatg
      841 tattcattgc gaccaatgaa aagggcaaac aaaccatgac ctcagcccgc agcgggcgta
      901 tcgaaatgct tgcccagggt cagtttttgc tgctcaacaa cggccaaaga cttgaaaccg
      961 tgaatggggc cgctaacctg aaaatcagtg aattcgaggt ttatggtgtt cagatcaaca
     1021 ccgatgtcac cagcaaatat cgctctgcgc cggtcaacac ccagacgacc ctcagtttgc
     1081 tgcagaaccc gactgccagc aatctggctg aactgtcatg gcgctttggc ctgatactgg
     1141 cagccgtcaa tttcgtcatc atcgggctgg ccgcatcggg cgtaaatccc cgcgctaaca
     1201 aaagtgtcaa tctggttttt tcgttgttca cttttgttgt ttattacaac ctcctcaatc
     1261 tggggcagag ttggatcgcc tcggaaaagc ttggcttcgc cacccatctg ctggctttgc
     1321 atggcggcat ctttctgatg gcaacgcttt ggcttgccaa gtgtcacaac aactggtcct
     1381 ggccccggct gaagaaggct gcatcttgaa aaccttgcgt cggctgattt acgctgaagt
     1441 catcaatgcc gttgcttttg ttgcgctggg atttttgtca ctgttttttt tctttgattt
     1501 tgtcgaagaa gtccagtcga ttgggcgaca ggcgttgccg ggttaccagt tgctgcaggc
     1561 gctgtcttat gtgaccttgc tgctgccaag tcacctctat gaactgatgc ccattgcggt
     1621 gctgattggc accatttttg tgatggccag gttggcccag agctcggagt acaccatttt
     1681 gcgcaccagc gggcttgacc cctggcgtgc gctcaggacc ttgcttgtgc tgggtgccgg
     1741 atttgtcatc gttacttttg ccattggcga ctatctatcg cccgtcgctg accgcactgc
     1801 ccagttcctc aaggcgcgct acaccgggca catcacattg gggcaaacag gtgcctggct
     1861 caaggaaaag caggatgccg ggcaatacgc tgtcaatgtg ggggagctcg cccctgacgg
     1921 cagcctgcgg ggcgtgcgga tatttgagtt caacaaccgg ggcacgttga tgtcgatgtc
     1981 actggctgag tcagccgtca tcgaggaaga cgatgcctgg cttctgaagc aggcacacca
     2041 gaccaagttt gccgtgcacg gtgattcatc ggcacagatt gaacgcgtgt tgctgccttc
     2101 attgcgctgg ccaaaccgga tcagtgccga gatggtgtca gccgccttgc tcaagcctga
     2161 ccggatgagc accatcgatt tgtttcagta cattgggcac ctgcaaaaca atgcccagac
     2221 cgcacaacgc tacgagatcg aattctggaa aaaagtgttt tatcccttaa gctgcctggt
     2281 catggtggtg ctggcgttgc cgtttgccta cttgaacttt cgctctggcg gcatcgccgc
     2341 atacgtgttt ggcggtgtca tggcaggcat cagctttttt ctgctcaaca acgtgtttgg
     2401 ctatatggga gatttgcgca actggcagcc ctggctgaca gccgcactgc caggcttgat
     2461 ctactcggcg ctgtcattga cagcgtttgg ctggctggtg ctgcggcgct agttcttctg
     2521 gaactttgac aaaagggtga attgatgcag gctacggttc tttttggtca tggctccagt
     2581 gacccggcgt ggcgaattcc gattgaccag gttgcccaga ccatgttatt ggcagacccg
     2641 caggcactgg tttgctgcgc gtttctggaa cgtaccaagc ccgacctggc aagcaccgtg
     2701 gctgatctgg cgcaaaaagg ggtaacccat gtcacgatcg tcccgatgtt tttgggcatc
     2761 gggcgccatg cgcgcgaaga catgccgatg ctggtcaaaa gcttgcaggc cgtttacccc
     2821 aaactgaatt tccgcctcaa gccgtcggtg ggtgaagagc cagaggtcat cgcgctgctc
     2881 gcacgcattg ccagacccac caccaatccg ttcgctgcct gaagatgaat ttgcatcaat
     2941 tccggtttgt acaggaagcc gcccggcgcg accttaattt gaccgaggcc gccaaagcgc
     3001 ttcacacttc gcagcccggt gtttcaaagg ccatcattga acttgaagac gagctgggca
     3061 ttgagatttt cgcccgctac ggcaaacggc ttaaacgggt caccgagccc ggacaattgg
     3121 tgctgaaaag catcgatctc atcatgcgcg aggtcaacaa cctcaagcgc gtcggcgagc
     3181 attacagcct cggtgacagt ggcacgctgt caattgccac cacccatacg caggcgcgct
     3241 actttctgcc tgagccggtt gcaaagttgc ggcaaaccta ccccaaggtc aatatcgcct
     3301 tgcaccaggg ttcacccgac gaggtggccc gcatggtgct tgaagaggtc gcagaagttg
     3361 gtattgccac cgagtcattg gccaaatacg acgatctggt caccctgcca tgctatgaat
     3421 ggcaccatgt gctggtgttg cccaccggac acccgctggc cagcaaagat cacctgacac
     3481 tcgaagacat tgccaaagag ccaatcatca cctaccaccc ggcctacacc gggcgcacca
     3541 agattgatca ggcctttggt gtgcgaaagc tcacgccccg cttcgccctg gaagccattg
     3601 actctgacgt gatcaagact tatgtgcgcc ttgggctagg cgtcggcatt gcggcggaaa
     3661 tggcggtgcg cgacatcgac aacgacggcg gcaaaagtgg tctgctggtg cgctctgtgg
     3721 gccagttgtt tggcaccaac gtggcgcggg tggcgttcaa gcgcggtgcc tatttgcgca
     3781 attttgtcta tcagtttgcc gagctgctca gtcaccggct tgaccgcaac ctggtggcgc
     3841 gtgccatgac aggtcaggtc aatgactacg agctgtaacc caggccattt gttttcaagc
     3901 gaccatcatg tcttgtcccc gccttcaatc caaattgccc cgcgtgggca ccaccatctt
     3961 cacggtcatg tcagcgcttg ccagcgaaac aggtgcggtc aacctgggcc agggttttcc
     4021 ggacttcgcc tgtgacccaa ggctggtgga tgccgtcacc ggggccatgc aaaaaggcct
     4081 gaatcaatac ccgccaatgg ccggtgtgcc agcgctgcgc gatacagttg ctatgaaaat
     4141 agaagctatg tacgcacgcc agtattgcgc tgcaaccgaa ataaccatta cagccggagc
     4201 cactcaggcg attttgacga tcatcctggc gatcgttcac aacggcgacg aggtgatcgt
     4261 gctggagccc tgctacgaca gttactgccc caatatcgaa ctggctggcg gcgtgccggt
     4321 gcgcgtgccg ctgacccccg gcacgtttcg cccggacttt gagaaaatcg ccgcagccat
     4381 cacgcccaga acccgcgcca tcatcgtcaa ttccccgcac aaccccagcg gcactgtctg
     4441 gtcgcatgac gacatgcaac acctggaaaa gctgctggca gccaccaatg tcatgctgat
     4501 cagtgacgaa gtctatgaac acatggtgtt tgatggccag cagcaccaaa gcgctgccag
     4561 gtttgccggc ttggccgagc gcgcctttgt ggtgtcgagt tttggcaaga cctaccatgt
     4621 gaccggctgg aaagtgggct atgtggcggc acccgctgaa ttgacacatg aatttcgcaa
     4681 ggtgcatcag ttcaatgtgt tcaccgtcaa tacaccggtg cagcacgcgc tggcccaata
     4741 catggccgac ccaaaaccct atctggagct gcccgacttt taccagcaaa aacgtgacct
     4801 gtttcgtgcc gggctggaac acacccgttt caagctgctg ccctgcgagg gaacttattt
     4861 tcagtgcgtc gatacgtctt ccatcaacac gctcgatgaa gcacggtttt gcaaatggct
     4921 gacctccgag gtgggcgtgg cagccattcc cctgtcggcg ttttacgcaa acagctttga
     4981 ccagcacgtt gtgcgctttt gt
//
LOCUS       DAOSLL010000341         4987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15041_length_4987_cov_4.257908, whole genome shotgun sequence.
ACCESSION   DAOSLL010000341 DAOSLL010000000
VERSION     DAOSLL010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15041_length_4987_cov_4.257908"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="PLB25_14175"
     CDS             complement(<1..751)
                     /locus_tag="PLB25_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOE42758.1"
                     /translation="MKTLVLFNYDWDLGGFARNAQAWPHDGAGFDLFSFPSNAHLAWF
                     DMTRYVARLARLAPRRGWTAVVSNHEQFGALAAALLAERMGWPGTPVNAVLTCQHKLY
                     AREVMAQVCPDANCGFSRLPMRYGDPVPEGLHYPSFVKPVKAAFSVLARTVLSRADLH
                     AHTRFGFWELWVIRHLVEPFERIMRQRLPQAGTAHSLMLEEPVPGAQFNLDGYAFAGE
                     VHALGVVDAVHYPGTQAFMRFDYPGTLAADIQ"
     gene            1072..1460
                     /gene="ssrA"
                     /locus_tag="PLB25_14180"
     tmRNA           1072..1460
                     /gene="ssrA"
                     /locus_tag="PLB25_14180"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1914..2441)
                     /locus_tag="PLB25_14185"
     CDS             complement(1914..2441)
                     /locus_tag="PLB25_14185"
                     /inference="COORDINATES: protein motif:HMM:NF021604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HOE42759.1"
                     /translation="MTDTPATSPENTPPPCTATGWRIATIVCLIALAIAATTAMSMFQ
                     QFKAQMEHLQAKMKNLPQIKYVSVLVDDKNAPAQLITFDPLDNFVQIQRVNDIKEGPN
                     DSMQLWAIPTAGKPRSLGVVAPQIKTAQLPATSKMLADITQLAISVENKGGVETGKEP
                     RLPYLFKGALIQKAM"
     gene            complement(2489..3823)
                     /locus_tag="PLB25_14190"
     CDS             complement(2489..3823)
                     /locus_tag="PLB25_14190"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HOE42760.1"
                     /translation="MKRFIPPLLSALQDNPHHRRLAVALGALLLASGVTAYSIAPLSP
                     DVANLPVRQLIENIAPIDTSAQRDSELVAPMVLYRSEITRPSDSLNTLFRRLGVSDIA
                     AASFLSRDTASRTLLAGTPGKFVSIEADDKQQLQRLTARWPSEDGAQFSRLVVERGSK
                     GYTSRVEMGQLERSVQLTSATVRTSLNAAAKEAKLPKPIAAQLAGVIARVPDFRVDLS
                     AGDSFGVVYETLEVDGEILRTGKLLGAEFVKNGKQHQVMWFQEPGQKGGFYTLNGQSI
                     DRDTMSLPLDTPKVSSGYGMRIHPVYGQAKAHEGTDFASPSGTPIRSAAQGVVTFAGW
                     KQGYGKFVLVKHRDQKATAYAHLSQIQVRKGQRVAMGDLLGAVGQTGTATGPNLHFEY
                     LVKGRPQDPQEISRQSAEKSITLTTRRDFERVAQTMRRQLDAASLVAQASAD"
     gene            complement(3830..4135)
                     /locus_tag="PLB25_14195"
     CDS             complement(3830..4135)
                     /locus_tag="PLB25_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008651884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HOE42761.1"
                     /translation="MKCPQCTEATLVMTERQGIEIDYCPSCRGVWLDRGELDKLIERS
                     AQAMPAQPPTPPQRAQPHYEQPMRRPDFVDSDYKHHSSHGSHDGYRKRKSWLGELFD"
     gene            complement(4132..4656)
                     /locus_tag="PLB25_14200"
     CDS             complement(4132..4656)
                     /locus_tag="PLB25_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HOE42762.1"
                     /translation="MKKALIPLGIFIVLLIFLGVGLTRDPRVIPSPLIGKPAPLFTAP
                     LLQAPDQTFTPKDMLGKVWLLNTWASWCVACQQEHPILVKFAKTKTIPIIGLDYKDKE
                     ADGLKWLGRYGNPYDVTISDKDGRIGIDFGVYGVPESFLIDKTGIIRYKQIGPITDEA
                     LRDKIIPLIRELQK"
     gene            complement(4818..4970)
                     /locus_tag="PLB25_14205"
     CDS             complement(4818..4970)
                     /locus_tag="PLB25_14205"
                     /inference="COORDINATES: protein motif:HMM:NF019403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HOE42763.1"
                     /translation="MLKQRPEWLVQPVPKALHVLARLGRWSLSYYLIHQPVLIALVSG
                     VSWILL"
BASE COUNT         1137 a         1298 c         1359 g         1193 t
ORIGIN      
        1 cctgaatgtc tgcggccagt gtgccgggat agtcaaagcg catgaacgcc tgggtgccgg
       61 ggtagtggac ggcatcgacc acgccaagcg cgtgcacttc gcccgcaaag gcgtaaccgt
      121 caagattgaa ctgcgcaccg ggtacaggct cttcaagcat caggctgtgc gccgtgccag
      181 cttgtggcag gcgctggcgc atgattcgct caaacggttc caccaagtgg cggatcaccc
      241 ataactccca gaagccaaag cgggtgtgag cgtgcaggtc tgcgcggctg agcacggtac
      301 gcgccagcac cgaaaatgcg gctttgaccg gcttgacaaa acttgggtaa tgcagcccct
      361 cgggtaccgg gtcgccgtag cgcattggca ggcgcgaaaa gccgcaattg gcatccgggc
      421 agacctgcgc catcacttcg cgcgcgtaca gtttgtgttg gcaggtcagc accgcattga
      481 ccggtgtgcc cggccagccc atgcgctcgg ccagcaaggc tgcggccagc gcaccaaact
      541 gctcatggtt ggagaccact gccgtccagc cgcgtcgtgg tgccagacgt gccaagcggg
      601 caacatagcg cgtcatgtca aaccaggcca gatgggcgtt gctgggaaag ctgaacagat
      661 caaaccctgc cccatcatgc ggccaggcct gcgcgttgcg ggcaaagcct cccaagtccc
      721 agtcgtagtt aaataacacc aatgttttca tgcgccaagt gtcgcatcca acatcacggt
      781 tgcacacgcc gtcgcgtcgg acgggtcact gaccagcgaa ttttgggtgg catgcggcac
      841 aggcaaagac tacctgacaa ctgatgtggc aagaacagag ctacaaatga gatcgccagc
      901 taggagcttg gcggcaaaca agcagctctc actactgagg ttcaagtctt tttcaaccaa
      961 gcagagcgcc tgcaaacgca taccgatctg gccggactct cgcaactgaa gctcaagtct
     1021 ttttcgacca aggccttgca acttttgcag atcgcctaaa ataagagcct tggggctgca
     1081 ttggtttcga cgcgggttcg gacgcgcagc agggcatgtc gagttgaatg tactcgttaa
     1141 accgattcaa aaaaactaac tgcaaacgac gaacgtttcg cactcgccgc ttaatcgcgc
     1201 gtgagcctcg caacagcaag ccgataggct gggcaagggt taccaagctg caaagctcgg
     1261 tagtccaggc tgcggggtaa tttgattcgg ctgggtctgc ctcgggtaac ttgaggcagg
     1321 tcgagaaaat aggttgctgg cgttttgcat agcgtgccac tgcgcgatgc aaaaggcgag
     1381 acttaaatca gacggctaca catgtagaac tgttcgaaca aggcttgcgg acgggagttc
     1441 aaatctcccc agctccacca tacatagaaa gcccaactgt tttcagttgg gctttttttc
     1501 gtctaaaaac tcaatggtgg cgcgcactta cggcgttcgc cttgtgactt ttctgctatg
     1561 aaaatcagtc gttttttgcc gtttttgacc aaaatcaccc cgttttctct ctctgttctc
     1621 agtgaccctc gtgacttttc acgaggccac cttcaataaa atcaacgact tacgcggggg
     1681 tggtttgcaa gttgaaattg gtgggcggta acgactgaga atagagaatg cctgaaatac
     1741 gtgtgtttgg ccgcctcgac aacacggtat tggcttgctc tgcgggggca ggcttgtgct
     1801 gatgcttgac caacgtcaaa agcagctggt cgaaactgct accagcaatt caaagtcgca
     1861 gtttgagtgg tttctgtttt tggttttttt gtcgcaagta aaaaaccaaa aaattacatc
     1921 gctttctgaa tcaatgcacc tttgaacaag tagggcaaac gaggctcttt acccgtttcc
     1981 acgccacctt tgttttcgac gctgatcgcc aattgcgtaa tgtctgccag cattttgctg
     2041 gtagccggaa gctgtgcggt tttgatttga ggtgccacaa cgcccaacga acgaggctta
     2101 ccagcggtcg gaatagccca gagttgcatg ctgtcgttag gcccttcttt gatgtcattt
     2161 acgcgttgaa tttgaacgaa attgtctagt gggtcgaacg tgattaattg cgcaggtgca
     2221 tttttgtcat ctaccagcac agaaacatat ttgatctgtg gcagattctt cattttggct
     2281 tgcaagtgtt ccatttgcgc cttgaactgt tgaaacatgc tcattgctgt cgtggcagca
     2341 atagcgagtg ctatcaaaca cacaatagtg gcgattcgcc agcctgtagc agtgcaaggt
     2401 ggtggtgtat tttcaggaga ggttgcaggc gtgtctgtca tgatggtcag gattacgttg
     2461 ttggggtgaa cagcagatgc tctgcaagtt aatcggcact ggcttgtgcc acgagagatg
     2521 cggcatcaag ctgacgacgc atggtttgcg ccacacgttc aaaatcgcgt cgggtcgtta
     2581 acgtaatcga cttttcagcg gattggcgcg agatttcttg gggatcttgt gggcggcctt
     2641 tcaccagata ttcaaagtga aggttgggtc cggtggctgt tcctgtctgt ccgacggctc
     2701 ccagcaagtc acccatggcc acgcgctggc ccttacggac ttgaatttgg ctcaagtgtg
     2761 cgtaggccgt ggctttctgg tcgcggtgtt tcaccagtac aaatttgcca taaccctgtt
     2821 tccaacccgc aaatgtcacg acaccttggg ccgcactgcg tatcggagta cccgagggtg
     2881 atgcaaagtc agtgccctca tgggccttgg cctgcccata gacggggtga atgcgcatac
     2941 cgtagccgct gcttaccttg ggtgtatcta gtggcagcga catggtgtca cggtcaatgc
     3001 tttgaccatt cagggtgtaa aagccaccct tctgaccggg ttcctgaaac cacatcacct
     3061 gatgttgctt gccgttcttg acgaactcag cacccagcag cttgccagtg cgcaagatct
     3121 cgccgtcgac ctcaagcgtt tcgtaaacca cgccaaaact atcacctgca ctcagatcta
     3181 ctctgaagtc gggcactctt gcaataacac cggccagttg cgcggcaatg ggtttgggta
     3241 gcttggcttc cttggctgca gcgttcaacg aagtgcgaac cgttgcgctg gtaagctgta
     3301 ccgatcgttc caattggccc atttccaccc gtgaggtgta tcccttggag ccccgttcga
     3361 ccaccagacg ggaaaactgg gcgccatcct cactgggcca gcgcgctgtc aggcgctgca
     3421 gttgttgctt atcgtctgcc tcaatcgaaa caaatttgcc aggagtgccc gccagcaagg
     3481 tacgcgatgc cgtatcgcga cttagaaagc ttgctgcagc gatatcgctg acacccaatc
     3541 gccgaaacag ggtgttcaaa gaatctgaag gacgggtgat ttcgctgcgg tagagcacca
     3601 tcggtgcgac cagttcactg tccctttgag ctgatgtatc gatgggtgca atgttctcaa
     3661 tcagttgccg cacgggtagg ttggcgacat cgggtgacag cggtgcaatg ctgtaggccg
     3721 tgacccccga tgccaataac aaggcaccta gcgcaaccgc caagcgacgg tgatgcgggt
     3781 tgtcttgcaa ggcagataaa agtgggggga tgaatcgttt catggcagtt caatcaaaca
     3841 attcacccag ccatgacttg cgcttgcgat aaccgtcgtg gctaccgtga ctgctgtggt
     3901 gtttgtaatc cgagtccaca aaatcggggc gacgcatagg ttgctcgtaa tgtggctgag
     3961 cccgctgcgg tggtgtgggc ggctgggctg gcatggcctg ggcgctgcgc tcaatcagct
     4021 tgtcaagttc accacggtcc agccacaccc cgcggcatga tggacagtag tcaatctcga
     4081 taccctggcg ctcagtcatg accagcgtgg cttcggtaca ttggggacat ttcatttttg
     4141 cagttctcga attagaggaa taattttgtc gcgcagtgct tcatcagtga ttgggccaat
     4201 ttgcttgtag cggatgatcc ctgttttgtc gatcaagaaa ctctcgggta caccgtacac
     4261 cccaaagtca atgcctatgc gtccgtcttt gtcggagatt gtcacgtcat aggggttgcc
     4321 gtaacgtccc aaccatttca gaccgtcggc ttctttatcc ttgtagtcca gtccaatgat
     4381 gggaatggtt ttagttttgg caaatttgac caatattgga tgttcctgtt gacaagccac
     4441 acaccatgaa gcccaggtgt tgagcaacca gacttttcca agcatgtctt taggcgtgaa
     4501 tgtttggtct ggcgcctgca gaagaggtgc agtaaaaagt ggtgcaggtt taccaatcaa
     4561 cggagatggg atgacgcgtg gatcacgtgt caaaccaaca cctaaaaaaa ttagcagaac
     4621 aatgaatatg cccaagggaa tcaacgcttt cttcattatt caactctctt tggtaaggat
     4681 gcatcggcag cacacatcat tgatgcataa gatgattcga tttgcgtaaa gaatcagtag
     4741 cggctccatt tcagttcctt tttcacgtgc ttttttggtt agaaaatcga cttgttatga
     4801 aaacaacggt ggaatgatta gagaagtatc cagctcacgc cgctcaccaa agcaatcaac
     4861 acgggctggt gtatcaggta gtagctcaag ctccaacggc ccagacgggc aagtacatgc
     4921 agagccttcg gcaccggttg caccaaccac tcgggccgct gctttagcac ccagcttcca
     4981 aaggctg
//
LOCUS       DAOSLL010000342         4987 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15042_length_4987_cov_3.897405, whole genome shotgun sequence.
ACCESSION   DAOSLL010000342 DAOSLL010000000
VERSION     DAOSLL010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15042_length_4987_cov_3.897405"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..366)
                     /locus_tag="PLB25_14210"
     CDS             complement(<1..366)
                     /locus_tag="PLB25_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE42764.1"
                     /translation="MNWFEDFETRHFDVNGARIHARVSRGVCGNRAVLLLLHGFPQSH
                     VMWHRVARKLQQDFYLVMPDLRGYGDSSKAPGLPDHSSYSKRSMAQDLVDVMDLLGIV
                     QFGVCGHDRGGRVAHRLALD"
     gene            complement(386..874)
                     /locus_tag="PLB25_14215"
     CDS             complement(386..874)
                     /locus_tag="PLB25_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOE42765.1"
                     /translation="MHPNEQTITRLYTAFAALDADAMAACYAEDASFDDPGFSLNGRR
                     EVAGMWRMLCDATRGKARQDWRLEFRDVRADGDTGHAHWEAYYRFSVTKRLVHNIIEA
                     EFTFTPDGLIATHKDSFDLWRWSRQAIGLGGLVLGWTSYFKKQMRLQTRAALTKYLAD
                     KT"
     gene            complement(949..1545)
                     /locus_tag="PLB25_14220"
     CDS             complement(949..1545)
                     /locus_tag="PLB25_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE/ArgO family amino acid transporter"
                     /protein_id="HOE42766.1"
                     /translation="MNFLQGFASALALIVAIGSQNAFILRQGIRREHVAALVIFCALS
                     DALLIVAGIGGAGALLRGNEALMLVARYGGALFLAGYGVLAARRAWRGNQMLPDNSSR
                     VSLATALAACFGFTFLNPHVYLDTVLLLGSIANQRPDPGRWVFGAGACLASVVWFSAL
                     GFGARFLAPVFQTTAAWRVLDSLIALTMLALAWSLLTV"
     gene            1732..2334
                     /locus_tag="PLB25_14225"
     CDS             1732..2334
                     /locus_tag="PLB25_14225"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOE42767.1"
                     /translation="MKTESNYRPLPKTPVSTEGCHLLDAFNPPAVNVDSAALQVMTDL
                     SQVPSATITADATLDDANHSMLVRGVRMLFVVGEDKHTIVGLVTSIDILAEKPVLVAQ
                     RRELKRSDLRVADIMTPVDKLEAVGIDDVRAANVGSILATLKLDGRAHAIVVGPGPQG
                     QQALLGIFSASQIARQLGVQVHAHEMARTFAEIEAVIAGV"
     gene            2554..3174
                     /locus_tag="PLB25_14230"
     CDS             2554..3174
                     /locus_tag="PLB25_14230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOE42768.1"
                     /translation="MDFKPLVTLLAIVNPLAIVPFFIHYTQDFTPEQRSRTIWIASFS
                     SFLVIATSALMGLHILAFFGISLASFQVGGGMLLLTSALSMLNAQPAEAKSNAEEMRD
                     AATRTSIAVVPLTIPLLTGPATMSTVVIYAEKAKTFWQLSALVSYGVVVALATALCFS
                     LAQPIARGLGKTGINVMTRLMGLILAALAVEVMSDGLIKLFPVLAK"
     gene            3253..4683
                     /gene="pyk"
                     /locus_tag="PLB25_14235"
     CDS             3253..4683
                     /gene="pyk"
                     /locus_tag="PLB25_14235"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HOE42769.1"
                     /translation="MQLRTTKIVATLGPASNDPALLEQMIRAGVNVVRLNFSHGTAQD
                     HIERARMVREAAARAGQEVAIMADLQGPKIRVGKFAEGKVFLEPGQTFVMDASRTDLG
                     DSGGVGLDYKSLPREVKPGDVLLLNDGLIVIIVNEVRGQAIHTTVKLGGELSNNKGIN
                     KQGGGLSAPALTGKDMDDIRTAMSFKADYVAVSFPKSATDMEMARQLCNVAAEDGHRP
                     GLIAKIERAEAIPKLEEILLASDGIMVARGDLAVEVGNALVPGLQKRMIKLALEHDRV
                     VITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMLSAETAAGKYPLETVIEMAKI
                     CHAAEAGRQLKFEADFVGIKFTRIDQTIAMGALFTAHHLGAKAIVALTESGSSALWMS
                     RHLIRVPIYALTSKIASQRKMTLFRNVRPLLIDTSTDRDTALAQAESDLKKRSIVQTG
                     DVYVITCGEPMGSPGGANLLKICRVG"
BASE COUNT         1093 a         1488 c         1435 g          971 t
ORIGIN      
        1 gtccagcgcc agccggtgcg cgacccggcc gccgcggtca tgaccacaga caccaaactg
       61 cacgatgcca agcaagtcca tcacatcaac caggtcttgc gccatgctgc gcttgctgta
      121 gctgctgtgg tctggcaaac cgggcgcttt gctggagtcg ccatagcccc gcaaatcggg
      181 catgaccaag taaaaatctt gctgcagctt gcgcgccacc cggtgccaca tcacatggct
      241 ttgtggaaaa ccatgcagca gcaacaggac agcccgattg ccacacacgc cgcgcgacac
      301 ccgggcgtga atgcgtgcgc cattgacatc aaaatgccgt gtctcgaaat cctcaaacca
      361 gttcatgctc aatcctgtct gcgggtcagg tcttgtcggc caggtatttg gtcagcgcgg
      421 cacgggtttg caagcgcatc tgctttttga aatacgaagt ccagcctagc acaaggccac
      481 caagaccgat tgcctgacgg gaccagcgcc acaaatcaaa gctgtccttg tgcgtggcaa
      541 tcaggccatc cggggtaaaa gtgaactcag cctcgatgat gttgtgcacc agccgcttgg
      601 tgacgctgaa gcggtagtaa gcttcccaat gggcgtggcc ggtgtcgcca tcggcgcgaa
      661 catcgcggaa ttcaagccgc cagtcctgcc gggccttgcc gcgtgtggca tcacacaaca
      721 tgcgccacat gccagccact tcgcggcggc cattgagcga aaaaccgggg tcgtcaaagc
      781 tggcatcctc ggcgtaacaa gcggccatgg catcggcatc cagcgcggca aaagcggtgt
      841 aaaggcgggt aatcgtctgt tcgttggggt gcatgggttt ctcggtttca tcacttgggg
      901 cttaggggtc gcctgcaaat cataaactgc gcggccctgc cacgtgattc aaaccgtcag
      961 cagcgaccag gccagcgcca gcatggttag cgcaatcagg ctgtcaagca cccgccaggc
     1021 ggcggtggtt tgaaacaccg gagccagaaa ccgtgcgcca aaacccaacg ccgaaaacca
     1081 gaccacgctt gccaaacacg ctccggcccc aaacacccag cgtccggggt cgggtcgctg
     1141 gttggcaatg ctgcccaaca gcagcactgt gtcaaggtaa acatgcgggt tcaggaaggt
     1201 aaagccaaaa caggcagcca gcgcggtggc caggctgacc ctgctgctgt tgtcgggcag
     1261 catctggttg ccccgccagg cacgccgcgc tgccagcacc ccatagccag ccagaaacag
     1321 cgcaccccca taacgggcga ccagcatcaa cgcctcgttg ccgcgcagca aggccccggc
     1381 accgccaatg ccagcgacga tcagcaacgc atccgacagc gcacaaaaaa tcaccagcgc
     1441 cgccacatgt tcgcgccgga tgccctggcg caggatgaaa gcgttttgtg agccaatggc
     1501 cacgatcagc gccagagcgc tggcaaaacc ctgcaaaaag ttcaaaatat atgcctaaaa
     1561 atggctctag cccttaccct gaaagcgcta gtagctatca tatttatatc attcatgaca
     1621 ccctgttgac ctcagccagg gccctgaagc acctgatttt ggttgacaag ttgtcgcaaa
     1681 aaaaccgcaa ttttgctacc atcagttgat caactaaccc aaggtgatgc catgaaaaca
     1741 gaatcgaatt atcggccctt gccaaaaacg cccgtttcca ccgaaggctg tcatttgctg
     1801 gatgcgttca atccgcccgc cgtgaacgtt gactctgcgg cgctgcaagt gatgacggac
     1861 ctgtctcagg tgccgtcggc caccattacc gccgatgcca cgctggatga tgccaaccat
     1921 tccatgctgg tgcgtggggt gcggatgctg tttgtcgtgg gagaagacaa gcacaccatc
     1981 gtcggcctcg tcacgtcgat cgatatcctg gcggaaaaac cggtgctggt cgcgcaaagg
     2041 cgtgaactca aacgcagtga tttgcgggtt gccgacatca tgacgccggt tgacaaattg
     2101 gaggcggtgg gcattgacga tgtcagagca gccaatgttg gcagcatcct ggctaccttg
     2161 aaactcgatg gtcgggcgca tgccattgtg gtcggaccag ggccgcaggg tcaacaggcc
     2221 ttgctgggca tcttttctgc ctcccaaatc gcccgtcagt tgggcgtgca ggttcatgcc
     2281 cacgaaatgg ccagaacctt tgccgaaata gaagccgtca tcgctggcgt ttgagctgtc
     2341 ggcaaacctg ccgcatggca ggcagagtcg ctcgcacgag ccaatagtgg cccgcatcac
     2401 tggtcaggtc agcagccggg ccaacaatgc cagtgtgcgc ttgtgtgcca gcgcggcggc
     2461 tggcgcgctg tgagtggcgc gatggtcaca aatgcagtca tgactgactg aggtcatgcc
     2521 acagcggcat cagatgcgac taccatgccg cccatggatt tcaaaccgct cgtcactttg
     2581 ctggccatcg tcaacccgtt ggccatcgtg ccatttttca tccattacac gcaggacttc
     2641 acgcccgagc agcgtagccg cacgatctgg atcgcatcgt tcagcagctt cctggtgatt
     2701 gccacaagcg cactgatggg tctgcatatt ctggcgttct ttggcatctc gctggccagt
     2761 ttccaggtcg gcggcggcat gttgctgctg acctcggcgc tgtccatgct caacgcgcag
     2821 ccagccgagg ccaagtccaa cgctgaggaa atgcgcgatg cggccacccg caccagcatt
     2881 gccgtggtgc cgctgaccat tcccttgctg accggccccg ccaccatgtc caccgtggtg
     2941 atttatgccg agaaagccaa gacgttctgg cagttgagcg cgctggtgag ctacggcgtt
     3001 gtcgtggcgc tggcgaccgc actgtgcttt tccctggccc agccgattgc tcgcggcctc
     3061 ggcaagactg gcatcaatgt catgacccgg ctgatgggcc tgatcctggc ggccttggct
     3121 gttgaggtga tgtccgatgg gctgatcaag ctgtttccgg tacttgcaaa gtgaggggtc
     3181 gcaggtccgg tcgccgccat ctcagcactt ccatgtgaaa atcgcccatc caaattccat
     3241 caggaccgat tcatgcagct tcgcacgacc aaaattgtgg ccactttagg ccccgcatct
     3301 aacgatccag cgctgcttga gcagatgatt cgcgccggtg tcaacgtggt gcggctgaac
     3361 ttcagccacg gcaccgcgca agatcacatt gagcgcgccc gcatggtgcg cgaggcggct
     3421 gcacgtgccg gtcaagaagt tgccatcatg gcagatttgc agggccccaa aattcgcgtt
     3481 ggcaaatttg ccgaagggaa agtgtttctg gaacccggcc agacatttgt catggacgcg
     3541 tcgcgtaccg atctgggtga cagtggcggc gttggcctgg actacaagtc tctgccgcgc
     3601 gaggtcaagc ccggtgacgt gttgctgctc aatgacggac taatcgtcat catcgtcaat
     3661 gaggtacgcg ggcaggcgat tcacacaaca gtcaagctcg gtggtgagtt atccaacaac
     3721 aagggcatca ataaacaggg tggcggtctg tcagccccgg cactgaccgg caaagacatg
     3781 gacgatatcc gtactgccat gagcttcaag gctgactatg tggcagtgag ctttccgaaa
     3841 agtgccacag atatggaaat ggcgcgccaa ctttgcaatg tcgcggccga agacggccat
     3901 cggccgggac tgatcgccaa gatcgagcgg gctgaagcca ttcccaaact tgaagaaatc
     3961 ctgctggcca gcgacggcat catggtggcc cggggcgatc tggcggtaga ggtgggtaac
     4021 gccttggtgc ccggcctgca aaagcgcatg atcaaactgg cgctggagca cgaccgggtg
     4081 gtgatcactg caacccagat gatggagtcg atgatcacca atccggtgcc gacccgcgcc
     4141 gaggtcagcg acgtggccaa cgccgtgctg gacggaaccg atgccgtaat gttgtcagcc
     4201 gagactgcgg ctggcaaata tccgcttgaa accgtgatcg aaatggccaa gatctgccat
     4261 gccgccgagg ccggtcggca gctcaagttt gaagccgatt ttgtcggcat caaatttacc
     4321 cgcatcgacc agaccattgc catgggcgcg ctgtttaccg cccatcacct gggcgccaaa
     4381 gccatcgtgg ccctgaccga aagcggctca tcagcactct ggatgagccg tcacctgatt
     4441 cgggtaccga tctatgcgct gacttccaag atcgcctcac agcgcaagat gacgctgttc
     4501 cgcaatgttc gcccgctgct gatcgatacc agcactgatc gcgacacggc actggcacaa
     4561 gctgaaagtg acctgaaaaa acgcagcatt gtgcagacag gcgatgtgta tgtcatcacc
     4621 tgcggagaac ccatgggctc tcctggcggg gccaacctgc tcaagatttg ccgtgtcggg
     4681 taaagtcgtg tcggcccgct gtcgtcgggc accacgccag agtcttcggc agcgggttgc
     4741 gcaaggtgcg ggcgtggggg acttggaaat agtcaacttc tgtcaaaaaa gtgcgcgact
     4801 tgcattaaca cagggctaga atttggcggt agatggatct gtgtctttgg tttcaacaag
     4861 taccgcccgg atatcttcgt gccgtgctcg tggcaaattg aaacatgaga tctttccaca
     4921 aagttcaccc gatgccacac ccccagattc caccagaaaa agcgacggtt agttaccgta
     4981 gcctggc
//
LOCUS       DAOSLL010000343         4966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15125_length_4966_cov_3.753614, whole genome shotgun sequence.
ACCESSION   DAOSLL010000343 DAOSLL010000000
VERSION     DAOSLL010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15125_length_4966_cov_3.753614"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2231)
                     /gene="pheT"
                     /locus_tag="PLB25_14240"
     CDS             complement(<1..2231)
                     /gene="pheT"
                     /locus_tag="PLB25_14240"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HOE42770.1"
                     /translation="MQFPESWLREFCNPPLSTAQLSDTLTMAGLEVEERQPVAPPFSH
                     IVVGEIRQAIQHPNADRLRVCQVDVGGADLLTIVCGAPNARVGIKVPCALVGAELPPG
                     EDGKPFLIKIGKLRGVESFGMLCSARELKLSQDHGGLLELAPDAPIGQDIRLHLNLDD
                     TLFTLKLTPNLAHALSVFGVARELSALTGAPLKKPEFPAVPVTANDVLPVKVSAPDLC
                     GRFSGRIIRGVNPRATTPAWMVDRLARCGQRSVAALVDISNYVMFEFGRPSHIFDLDK
                     IHGGLEVRWGQPGEQLKLLNGTMVTVDAQVGVIADAQQVESLAGIMGGAATAVSDDTQ
                     NVYVEAAFWWPRAIAGRSRRFNFATDAGHRFERGVDANLTVEHIERITQLIIDVCGTP
                     DTVCGPMDDQQVVLPAPLTVTMRVARAAKVLGMPLTQMQCAKALSGLGLPVEEGDGII
                     SVVPPSYRFDLQIEEDLIEEVARMVGYDKLPETPPLAPITAKIRPESQRGEFALRRAV
                     AELGYQETINFSFVEERWEHELAGNPNPIKLLNPIASQMSVMRSSLLGSLLQVLRFNQ
                     DRKAPRVRVFEIGRVFLRDASIISSDTTVEGFDQPMRLAGLAVGANDAPEWSSKDRSV
                     DFFDVKGDLQALLAPERLTFVAAQHPAMHPGRCAQILKAGVVIGFVGELHPKWQQSYD
                     LAQAPVLFEMNLNDVLSRPVPVFKSVPKFQAVERDIAVLVGEHVTHDALMGAIWSAPT
                     DGL"
     gene            complement(2430..3506)
                     /gene="pheS"
                     /locus_tag="PLB25_14245"
     CDS             complement(2430..3506)
                     /gene="pheS"
                     /locus_tag="PLB25_14245"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HOE42771.1"
                     /translation="MNDLQTVVASARAAFELAQSPADLENTKAQYLGKSGCITAMMKG
                     MAALSVDAKKTQGAAINLAKQAIEAALNARRQAMADAELQLQLQTEALDVTLPGRQRG
                     QGGLHPVSLTLERIEAIFGSMGFEVAQGPEIESDWFNFTALNTPEDHPARSMHDTFYV
                     EGGTQAAPNLLRTHTSPMQIRHAVQHVKKHRLLAQGASAAVGLFAGAMPEIRVIAPGR
                     TYRVDSDATHSPMFHQCEGLWVGENVSFKDLKFVFTDFCRTFFESKDLVLRFRPSFFP
                     FTEPSAEIDIQFPAGPLAGRWLEVAGSGQVHPNVIRNMGLDPERYIGFAFGMGPDRLT
                     MLRYGVNDLRLFFDGDIRFLSQFR"
     gene            complement(3615..3974)
                     /gene="rplT"
                     /locus_tag="PLB25_14250"
     CDS             complement(3615..3974)
                     /gene="rplT"
                     /locus_tag="PLB25_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HOE42772.1"
                     /translation="MPRVKRGVTAHARHKKVLALAKGFRGRRGNVFRVAKEAVMKAGQ
                     YAYRDRRTKKRVFRQLWIARINAAARQCGMTYSQFANGLKKASIEIDRKVLSDIAVHD
                     MAAFAGIVDQVKARLAA"
     gene            complement(4000..4203)
                     /gene="rpmI"
                     /locus_tag="PLB25_14255"
     CDS             complement(4000..4203)
                     /gene="rpmI"
                     /locus_tag="PLB25_14255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HOE42773.1"
                     /translation="MPKMKTKSAAKKRFRVRPGGTVKRGQAFKRHILTKKTTKNKRHL
                     RGATAVHETDMGRMAQMLPGRGL"
     gene            complement(4448..>4966)
                     /gene="infC"
                     /locus_tag="PLB25_14260"
     CDS             complement(4448..>4966)
                     /gene="infC"
                     /locus_tag="PLB25_14260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HOE42774.1"
                     /translation="VRLSGVENEPLGVVSLTEALRMAGELDVDLVEIAATAIPPVCRL
                     MDYGKFKYQEQKKAAEAKAKQTVIEIKEIKFRPGTDDGDYNIKMRNIRRFLAEGDKCK
                     ITLRFRGREITHQDLGMALLNRIRDELGDAILVEQFPKLEGRQMIMMIAPGRKKQQPV
                     KAPVVESATAVA"
BASE COUNT         1117 a         1465 c         1324 g         1060 t
ORIGIN      
        1 aacaaaccgt ctgtcggggc ggaccaaatg gcccccatca gggcatcatg tgtgacatgt
       61 tcacccacca gcacggcgat gtcacgctca actgcctgga attttggcac tgatttaaaa
      121 actggcaccg gtcggctcaa cacatcgttc aggttcattt caaacaacac cggggcctgc
      181 gccaggtcgt aggactgctg ccatttgggg tgcagttcac caacaaaacc gatgaccaca
      241 cccgccttca aaatctgcgc gcaacgaccg gggtgcatag ccggatgttg ggccgcaaca
      301 aaggtcaaac gctcgggtgc cagcagggct tgcaagtcgc ctttgacatc gaaaaagtca
      361 accgaacgat ccttgctgct ccactcgggt gcgtcattgg cgccaaccgc cagcccggcc
      421 agtcgcatgg gttggtcaaa gccttcgacg gtggtgtcag aactgatgat cgaagcatcg
      481 cgcaaaaata cccgcccaat ctcgaacacc cgcacacgcg gcgctttccg atcctggttg
      541 aacctcaaaa cttgtagcaa tgaacccaac agggacgagc gcatcacgct catttggctt
      601 gcaattggat taagcagttt gatcggattc gggttgcctg ccagctcatg ttcccagcgc
      661 tcttcgacga aactgaaatt gatggtttcc tgatagccca attcggccac ggcgcggcgc
      721 agcgcaaact ccccgcgctg cgactccgga cggattttgg cagtgatggg tgccagtggc
      781 ggtgtctctg gcagcttgtc atagccgacc atacgggcca cctcttcaat cagatcttct
      841 tcaatctgca aatcaaagcg gtaggacggc ggcacgacgg aaatgatgcc atcgccttct
      901 tctaccggca agccaagacc gcttagtgcc ttggcgcact gcatctgcgt gagtggcatg
      961 ccgagcactt tggcggcacg cgctacccgc attgtgacgg ttagaggtgc gggcaggacg
     1021 acttgctggt catccatcgg accacaaacg gtatcgggag tgccacaaac atcgatgatc
     1081 agttgcgtga tgcgttcgat gtgctctact gtcaaattgg catcaacacc ccgctcaaag
     1141 cggtgcccgg catcggttgc aaagttgaag cggcgcgagc gcccggcaat cgcccggggc
     1201 caccagaacg ccgcttcgac atagacgttt tgcgtgtcat ccgagaccgc cgtggcagct
     1261 ccacccatga taccggccag cgactcgacc tgctgggcat cggcaatcac gccaacctgt
     1321 gcatctaccg tcaccatcgt gccattgagc aacttgagct gttcaccggg ctggccccag
     1381 cgcacctcca ggccaccgtg aattttgtca aggtcaaata tgtgtgacgg gcgcccaaac
     1441 tcgaacatga cgtaattgga aatatccacc agtgccgcca cactgcgctg accacagcgc
     1501 gccagccggt ccaccatcca ggccggtgtc gtggcgcgcg gattcacgcc gcgaatgatc
     1561 cgaccggaaa agcgaccaca cagatcgggg gcgctgactt ttaccggcag cacatcattg
     1621 gctgtcacag gtactgcggg gaactcgggc ttcttcaatg gcgcaccggt caatgccgac
     1681 aattcacgcg ccaccccaaa cacgctcaga gcatgcgcca ggttgggcgt cagcttgagg
     1741 gtaaacaggg tatcgtccaa attcaggtgc aggcgaatgt cctgcccgat tggtgcgtcg
     1801 ggtgccaact ccagcaaacc accatgatct tgcgagagct tgagttcgcg tgcggagcac
     1861 agcatgccaa agctctcgac cccgcgcagt ttgccaatct tgatcaaaaa cggcttgcca
     1921 tcctcgcctg gtggcagttc cgcgccaacc agggcacagg gtaccttgat accgacgcgg
     1981 gcattgggtg ccccgcagac gatggtcagc agatcagccc cgccgacatc gacctggcaa
     2041 acccgcagcc tatcagcgtt gggatgctgt atcgcctgcc tgatttcgcc aaccacaatg
     2101 tggctaaagg gcggtgccac aggctggcgt tcttcaactt ccagaccagc catggtcagg
     2161 gtgtcagaca gttgggccgt gctaaggggc gggttgcaga actcacgcaa ccaggattca
     2221 ggaaattgca taatcgtttt ctaaagtaaa aatacagatc aatgaccccc aattgcgtgc
     2281 catttcagcg tctgcggata tcgatggaac gttggatggc tgcctgaaca gaattgacgc
     2341 tggcgcttct ctgcttttgt cattgagatt gacagactgg tcagccagga atgcctttgc
     2401 cagcgctcaa atgcaggcga cagatcgagc taacgaaact gggacaaaaa ccggatatcg
     2461 ccatcaaaga acaaacgaag gtcgttcaca ccgtaacgca acatcgtcaa gcggtccggg
     2521 cccatgccaa aggcaaaacc aatgtagcgt tccgggtcaa ggcccatatt tcggatcaca
     2581 ttggggtgaa cctgaccgga gccagccact tcgagccagc ggccagccag cggaccagcg
     2641 gggaactgaa tgtcgatttc ggcgctgggt tcggtgaatg gaaaaaagct cggacgaaag
     2701 cgcagcacca gatctttgga ttcaaagaaa gtacggcaaa aatcggtgaa gacaaacttc
     2761 aaatccttga aactgacgtt ctcgccgacc cacaggcctt cgcactggtg aaacatcggc
     2821 gaatgggtcg catcactgtc cacccggtag gtacggcccg gcgcgatgac acgtatctca
     2881 ggcatggctc cggcaaacag accaacagca gcgctggccc cctgagccag caagcgatgt
     2941 ttcttgacat gctgcacggc atggcgaatc tgcatcggac tggtatgcgt acgcagcaaa
     3001 ttgggcgctg cttgcgtgcc accttcgaca taaaaggtgt cgtgcatgga gcgcgccggg
     3061 tgatcttccg gtgtattgag agccgtgaaa ttgaaccagt ccgattcaat ttcagggccc
     3121 tgcgccactt caaagcccat cgaaccaaaa atggcttcaa tgcgttcgag cgtcagcgac
     3181 accgggtgca gaccgccttg accgcgctgg cgacccggca atgtcacgtc cagagcctcg
     3241 gtttgcaact gtaactgcag ctcggcatct gccatagctt gtctgcgcgc attcaaggcc
     3301 gcttcaatcg cctgcttggc caaattgatg gcagcgcctt gggttttctt ggcatcgact
     3361 gaaagcgcag ccatgccttt catcatcgcg gtgatgcaac cagacttgcc caaatattgt
     3421 gccttggtgt tttccaggtc tgccggtgat tgggccaact caaaggcggc tcgggcgctt
     3481 gcgacaacgg tctgaaggtc gttcataaaa aatctggatt tctgtcatgg aaaaagggct
     3541 agtgcctttt caagctctag cccttgtcaa tactgcacac caagctatcg aaatgatagc
     3601 caactaacgc tagctcaggc ggccaaccgg gctttaacct ggtccacaat accggcaaac
     3661 gcagccatgt catgtaccgc gatatcgctg agcaccttgc ggtcgatctc gattgaggct
     3721 ttcttcagac cgttggcaaa ctggctgtag gtcatgccac actgacgggc agcggcgtta
     3781 atgcgggcaa tccacaactg acgaaagact cgctttttgg tacgacggtc acggtaggca
     3841 tattgcccag ccttcatgac cgcttctttg gcgacccgga aaacattgcc gcggcgacca
     3901 cggaagcctt ttgcaagggc cagaactttt ttgtggcggg cgtgagccgt tacaccacgt
     3961 ttgacgcgag gcattgaatt actccttgtt cgtcgttaat taaagaccac ggccgggcaa
     4021 catctgtgcc atgcgaccca tatcggtctc atgaacagcg gttgcaccac gcaagtggcg
     4081 tttatttttg gtggtcttct tggtcagaat gtgacgtttg aaggcttgac cgcgcttgac
     4141 ggtaccacct ggacgcacgc ggaagcgctt cttcgccgcg ctttttgtct tcattttggg
     4201 catttgcatg ctcctatttt cattgtgctc gtgaggcgtc tgcaaacact ttgcagcctt
     4261 gatggccccg agccactttt tgcagcggta tttatccacc actattgcag tgacgggcaa
     4321 gcccttcact gcggtcagca agcgtgaact tgccgccttt gacgcgagcc cttcaccact
     4381 ttcgtgacaa aaccagaccc acttcaatct gtcttagaac atcccgcacg ggatgtccgc
     4441 caaattgtta tgccaccgcc gtggcacttt caaccacggg ggccttcacc ggctgctgct
     4501 tcttgcgacc cggagcgatc atcatgatca tctgacgtcc ttcgagcttg ggaaactgtt
     4561 caaccaaaat ggcatcgccg agttcgtcac ggatccggtt cagcaaggcc atgcccaagt
     4621 cctgatgggt gatctcgcgc ccacgaaaac gcagcgtgat cttgcacttg tcgccttcgg
     4681 ccaaaaagcg ccgaatattg cgcatcttga tgttgtagtc gccgtcatcg gtaccgggtc
     4741 gaaacttgat ctccttgatt tcgatcacgg tctgcttggc cttggcctca gcagcctttt
     4801 tctgttcctg gtacttgaac ttgccgtaat ccatcaaacg acaaaccggt ggtattgcgg
     4861 ttgcagcgat ttccaccaaa tcaacgtcca actcccccgc catacgcaac gcctcggtca
     4921 ggctcacgac acccagcggc tcgttttcca caccagagag tcgaac
//
LOCUS       DAOSLL010000344         4942 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15216_length_4942_cov_4.105586, whole genome shotgun sequence.
ACCESSION   DAOSLL010000344 DAOSLL010000000
VERSION     DAOSLL010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15216_length_4942_cov_4.105586"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(431..2263)
                     /locus_tag="PLB25_14265"
     CDS             complement(431..2263)
                     /locus_tag="PLB25_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein/DEAD/DEAH box
                     helicase"
                     /protein_id="HOE42775.1"
                     /translation="MATLIPALAACTARMTSGERRLAQRLEDKLDDDYLLWYDVPVGA
                     SNAHPDFVVMHPRRGLLVLEVKDWKLAILQNVDKQSWTIIPDGIPKTVSNPLEQARQY
                     AHQVVDALKRDPQLVHADGKHQGNLAFPWSYGVVLTNITRKQFKAAQLQHAIEPNHVL
                     CQDEMLETVAAEDLQSRLWGMFPYMMRGVMSLPMLDRVRWIMFPQVRLQTQGTLFDDS
                     DAQAELPGIMHVMDLQQEQLARSLGDGHRVIHGVAGSGKTMLLGYRAEYLARHCAASS
                     KPVLVLCFNEPLGVNLHRVMQAKGLDDRVQVRHFHKWCRDQLVAFGQTLPSNKLTTEA
                     KMQDLVQRVISGVDRRQIPSGQYLAVLIDEGHDFAPEWLRLVTQMVDPGTNSLLVLFD
                     DAQSIYEHRASRDRVKPFSFKSVGIQAQGRTTILKINYRNTRQILQVASLIAADLLTP
                     DDKGDDGISLVKPVSCGREGEAPLIIRLPTLRDEAGAIADLFANAHKEGHAWGDMAIL
                     CADSKTRDLCAQVLARRKMPVENRLRPVDYDPGSNKIKVMTMMVSKGLEFAVVALPGV
                     GHMPGPGEDEREAARVFYVAATRATQRLVIGVEGNGGFGAKLGG"
     gene            complement(2636..2956)
                     /locus_tag="PLB25_14270"
     CDS             complement(2636..2956)
                     /locus_tag="PLB25_14270"
                     /inference="COORDINATES: protein motif:HMM:NF036549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42776.1"
                     /translation="MSDMVWSATEKKVARRVFDAALQRELADIMADFKTRAAQASTPD
                     DLWAIKAHLVRVQHDIDSKYDYRYSTLEFVLGRLLFEQRVQLQDLTGMKAERIAKICR
                     IASQ"
     gene            complement(2956..3402)
                     /locus_tag="PLB25_14275"
     CDS             complement(2956..3402)
                     /locus_tag="PLB25_14275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42777.1"
                     /translation="MTQQSSLPQHFAAAVLATIAVFLAVYTWLPSRDGLQLDEAVVSA
                     ITPQRNTWFEVALTTSSGKRITCRTRRGWPLLGPSRCPLAAFEALLGQSVSVLHDDQR
                     AYEVRAGSQIVVDYWAHRRQQTIALVLSGLLLVMALRVAGFSLRSR"
     gene            complement(4284..4610)
                     /locus_tag="PLB25_14280"
     CDS             complement(4284..4610)
                     /locus_tag="PLB25_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42778.1"
                     /translation="MARRKHYHVYVVELSKEVLANARFMRSNPSYQTGKPCVYVGMTG
                     LDPDVRFDKHKAGIQANRYVQVYGLRLLPALYALYNPLTYDEARSLEVELGIDLREGG
                     YGVWQA"
BASE COUNT          999 a         1482 c         1420 g         1041 t
ORIGIN      
        1 aattcgctcc ctgctgctca ataaccttac ccagcagcac tcccgcatca actccatgca
       61 acgcacttga tgcttctatt tagatagcgt ctatcgcaca ttggtattgg cctgcggcac
      121 aatttcactt caaacaccag tagcctgatg ccatttctga ttcattcgta tcccggcacc
      181 ttggcacagg ctttgcaaac gcaaggcgag ataacaacga catggatggt ttggaaatcg
      241 aacaagtcag acccgctacc ggatcgcact gcgtgcaaca agcgcatttg aagcatgttg
      301 aacgaggctg gactggcgcc tggcttgaat tgatggacag aggtgacttc cggcttgatg
      361 atcccgattc ctgccatgca aaacgagtcg gcaacgactt gcttgcctcc aatgccggtt
      421 gtcgggctga tcagccccca agctttgcgc caaagccccc gtttccctcc accccaatca
      481 ccaagcgctg ggtggcgcgg gtcgctgcca cataaaacac acgcgccgct tccctctcgt
      541 cctcgcctgg cccaggcata tgcccgacac cgggcagcgc caccacggcg aactccaggc
      601 ctttgctgac catcatggtc atgaccttga tcttgttgct gccagggtca tagtcgacag
      661 ggcgcagacg gttttcgacg ggcattttgc gtcgcgccaa cacctgtgcg cagaggtcac
      721 gggttttgct gtcggcgcac aaaatggcca tgtcgcccca ggcgtggcct tctttgtggg
      781 cgttggcgaa cagatcggca atcgcaccgg cctcgtcgcg cagggtgggc aaacggatga
      841 tcagcggggc ctcgccttcg cgaccgcaac tgaccggctt aaccagcgat ataccgtcgt
      901 cacccttgtc atctggggtc aacaggtcgg cggcgatcag actggcgact tgcagaatct
      961 gcctggtgtt gcgatagttg attttcagga tggtggtgcg gccctgcgcc tggatgccaa
     1021 cgcttttgaa actgaagggc ttgacccgat cacgtgatgc ccggtgctcg taaatgcttt
     1081 gcgcgtcgtc aaacagcacc agcaggctgt tggtgccggg gtccaccatt tgggtcacca
     1141 acctgagcca ttcgggcgca aagtcgtggc cttcgtcaat cagcaccgcc aggtattggc
     1201 cgcttgggat ctggcggcgg tccacgccgc tgataacgcg ttgcaccaaa tcttgcatct
     1261 tggcttccgt ggtcagcttg ttggacggca gggtttgccc aaaggccacc aattggtcgc
     1321 ggcaccattt gtgaaaatgc cgaacctgca cccggtcgtc aaggcccttg gcctgcatca
     1381 cgcggtgcag gttcacgccc aaaggctcgt tgaaacacag caccagcacc ggtttgctgc
     1441 tggcagcgca atggcgtgcc aggtattcgg cgcggtagcc taaaagcatg gttttgccgg
     1501 aacccgccac accgtgaatc acccggtggc cgtcgcccag actgcgcgcc agttgctctt
     1561 gctgcaagtc catcacatgc atgatgccgg gcagctcggc ctgcgcatcg ctatcgtcaa
     1621 acaaggtgcc ctgggtctgc agtcgcactt gcgggaacat gatccagcgc acccggtcca
     1681 gcatgggcag gctcatcacc ccgcgcatca tgtaggggaa catgccccac aggcggcttt
     1741 gcaggtcttc ggcagccacc gtttccagca tctcatcctg gcaaagcacg tggttgggtt
     1801 cgatggcgtg ctgcaattgg gccgccttga attgcttgcg ggtgatattt gtcagtacca
     1861 cgccgtagct ccacggaaac gccagattgc cctggtgttt gccatctgcg tgcaccagtt
     1921 gcgggtcgcg tttgagggcg tcgaccacct gatgcgcgta ttggcgggct tgttccagcg
     1981 ggttgcttac tgtcttgggt atgccgtcgg ggatgatggt ccagctttgt ttgtcaacat
     2041 tttgaaggat ggccagcttc cagtctttga cttccagcac cagcaggccc cggcgcgggt
     2101 gcatcacgac aaaatcgggg tgagcgttgc ttgcgccaac tggcacgtcg taccacagca
     2161 ggtagtcgtc gtcgagcttg tcttcaagcc gctgggccag ccgacgctcg ccgctggtca
     2221 tgcgtgcggt gcaggcagca agcgccggta tgagggttgc catcgttaat tttcattccc
     2281 tgaaatggtg tggctgatta tctgcctttg gcagcgcgcg ccagttcccg gccaatgcgg
     2341 atatttgagg cgcatgttcg ctccctgaga ctgcctgatg aagccggaat tttgactggc
     2401 acaacactgc cgcatggcaa gggatttgaa agtgttgacc ccttgctggg gatatggtca
     2461 gaaatagtcg cccttttttg caaagacgga tttgggcgca cagcgtcttt gcaagacgct
     2521 tgttgagggg tgccaacttt cagtgtcatt caagcctcta gcccttgttg tataagcaca
     2581 agcagctatt aatttgaaag cacctcaaga gtgctaaaag ccttctgctt gccggtcact
     2641 gcgacgcgat gcggcaaatt ttggcgatcc gctcggcctt catgccagtc aggtcttgca
     2701 gttgaacgcg ctgctcaaac aacagccgac ccagcacaaa ctcaagcgtc gagtagcggt
     2761 agtcatactt gctgtcgatg tcgtgttgca cccgaaccag gtgcgcctta atcgcccaca
     2821 ggtcgtcggg cgtgctggcc tgggctgcgc gggttttgaa gtctgccatg atgtcggcca
     2881 gttcgcgctg caacgcagcg tcaaagaccc gccgcgcgac cttcttttct gtggcgctcc
     2941 agaccatgtc cgacatcaac ggctcctcaa tgaaaagccc gcaacccgca gcgccatcac
     3001 cagcagcagg ccggaaagca ccagggcgat ggtttgttgc cttcggtggg cccaatagtc
     3061 caccacgatc tggctaccag ccctgacctc ataggcgcgc tggtcatcat gcaacaccga
     3121 aacgctttgg cccagcagtg cttcaaatgc cgccagcggg cagcgacttg gccccagcag
     3181 cggccagccg cgccgggttc ggcaggtaat gcgcttgccg ctgcttgtag tcagcgcaac
     3241 ctcaaaccag gtgttgcgct ggggcgtgat ggcactgacc acggcttcat caagctgcaa
     3301 gccgtcgcgc gacggcagcc aggtgtaaac cgccaaaaag accgcaatgg tggccaacac
     3361 ggcggctgca aaatgctgcg gcaagctcga ttgttgagtc atacgcggtg tgtgccttgc
     3421 ggcatgttgg gctgcgccat cctgatcggg ttgaagtcgg gaaacaacat tctttgccaa
     3481 gccagtaggg ccatcggcga attgacagca gaaatataaa ccagaccaga ccggcccggc
     3541 aatgcacaac atgggcaatg gcagggcaaa gttgttcttt cgctaagtcg ttgatttacc
     3601 gtcattcccg cgcaggcggg aatgacgaag atgccataag aacaaatttg ccctgtgggc
     3661 agtgggctgt gagccggtcg agctgacgga ccgggcatcg aggtcatcga ggctgtcagg
     3721 gccagtttgt gccggatttg ccgctccaca gcccattcgt gggtcagaaa gcaggtgcaa
     3781 gattggccgc agcggtcggc ttgcagttta tgcgccataa gccccgcaac tcttgaccag
     3841 atattgaagt tcaagacaac gccgcgcaag acaacgatcc agtgggcacc ggtctggtac
     3901 agaccacttc aagccgcgca ttgcattgcc atatcaatcc gacttgttgt tgcttcgcct
     3961 ggccgtgctg cagcactgtc tgcggcttcg cgcctgatgc tgcatcgatc tggaaacgca
     4021 atcaggcgcg ggtttaatgc ggcgaaagcc gaccagccaa tctttcagcc gcgtggcccg
     4081 tccgccgcaa gagcagcaag gcgtgacgag tgagggtcct aaacgatcaa aaaaagtgaa
     4141 tcacatagtc ctcgcgtcat gaacgacgca ccgtgcagct caaggtatag ggttgccagc
     4201 ggtacacatg ggccagcaat tgagcagcga aatggacatg cccggcgtgc cgatgccttg
     4261 tggccaacgc agtcgaactg gcatcacgcc tgccagaccc catagccacc ctcacgcagg
     4321 tcgatgccca gttccacttc gaggctcctg gcctcgtcat aggtgagtgg gttgtacaag
     4381 gcgtaaagcg ccggcagcag ccgaagccca tacacctgga catagcggtt agcctgaatg
     4441 cctgccttgt gtttgtcaaa gcgcacatcc gggtccagcc cggtcatgcc aacgtagacg
     4501 cagggcttgc cggtctgata gctcgggttg gaacgcatga accgggcgtt cgccagcact
     4561 tccttggaca gttccaccac atacacgtga tagtgcttgc gccgggccat gatcgcttga
     4621 ccgctgcgct ttcagcccac cagggacaac cgagcagaaa acactgccat tgcctgctgt
     4681 cgatccgcag ggtactgccg cagccaatcc aaaccgtcct gcgctctggc tgtgcgccac
     4741 gcgctacagc cgtccggcgg caagtacccc accctgcgct tttcatctac cggcatgtga
     4801 aactggcgct tcaacccatg gccatgcgca ttgatggtca gcatggtgaa gctgcccggc
     4861 aaaccctggg gtgacttcca gcatgcccgg cgactggcca tagcaggccc gccattcgca
     4921 ctgggcggtg gcaacagttt tg
//
LOCUS       DAOSLL010000345         4937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15234_length_4937_cov_5.364400, whole genome shotgun sequence.
ACCESSION   DAOSLL010000345 DAOSLL010000000
VERSION     DAOSLL010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4937
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15234_length_4937_cov_5.364400"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(367..675)
                     /locus_tag="PLB25_14285"
     CDS             complement(367..675)
                     /locus_tag="PLB25_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42779.1"
                     /translation="MMWGGAITLPDVQPFTFRKYRPPPALRRSTCDMCGAPVVGFLRL
                     APFVRLAFVQSANFPDQSVLPPTSVHIFYHSRVKDFEDDLPKVSGYWASELAVTKLLF
                     "
     gene            complement(884..1429)
                     /locus_tag="PLB25_14290"
     CDS             complement(884..1429)
                     /locus_tag="PLB25_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42780.1"
                     /translation="MRLKKSLSNLSHQVATLLLAGAVAAPSWAERIRFVSVIAPTAEA
                     KLVFADGSNRTLSLVQREGMTSGAGPLASAIMQEWGMHDIVPAQGGADGAGYLVFTLA
                     NGDKAYLKYQWHAIQVVQDGKARNLFDGHWGVVGSTGKLVGLQGIGTLYITPVSATGR
                     QWTFEGELVLNQAITGEKTQP"
     gene            complement(1484..2398)
                     /locus_tag="PLB25_14295"
     CDS             complement(1484..2398)
                     /locus_tag="PLB25_14295"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HOE42781.1"
                     /translation="MDIIDILHNWTEHQAMLVFAYSLIFFLVIDILSGALSDRRQYTM
                     QEMGLNVFAFVNFLTARALMVGVWVWIFLKAFPGGTGFMNGMGFWTTFFVYMLVEEYA
                     HYWIHRLSHSIPWLWRIHKAHHTPEHMNVTVTYRENWLWWALASNTYITAFFIWGGHI
                     APVVLAGSAKIIVGFLSHSGSRWDLALHKNRFTQPFMWVLERVVTLQDTHHAHHGIGK
                     YGNGMTNYAPFLFIFDVIHRTGIIPHTRQEAFGVPKTVKVEPWYVLLWWPVLKTAQPA
                     DLRPPTKADSEAQSRIYTSQNGRVTIKM"
     gene            complement(2433..3533)
                     /locus_tag="PLB25_14300"
     CDS             complement(2433..3533)
                     /locus_tag="PLB25_14300"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HOE42782.1"
                     /translation="MQVFKALFKGSSKAANQLSIEGTNFRTSMKPNQSVLTAAVDARF
                     KVPNYCGVGECGTCRCRVMDGRVRLTRDITNHISLEEIEQGYVLACQTIAESDLVIRV
                     PGLSPEDDSLVTSSATISALIPLTHDIFEVQVALERPLAYIAGQYAQLSVPAEPQLAE
                     TPRNYSFASAPGVGNANSASFYIRHVPGGLFTDWLFAADRTGARLTVTGPYGDFRYRK
                     TERPLLCVAGGSGLAPIKALLEQLRSEGISRQVIFLFGARQQRDLYCLDEIAEMAAAW
                     PVSFAFHPVLSHEPVNSNWTGRRGLVTDHVVQVVPNYADHDAYLCGPPPMVDAVIAKV
                     KGHIPADRLHFDKFLDQSALHPTTNAQDLVAA"
     gene            3650..4555
                     /locus_tag="PLB25_14305"
     CDS             3650..4555
                     /locus_tag="PLB25_14305"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOE42783.1"
                     /translation="MNLRKVDLNLLTVFDAVMREGNFTRAADKIGMTQPAVSEAVARL
                     RHLFKDDLFIRNGRGVQPTPRAHQYAAPIRRSLDLIAATLSESHTFDFVTSTRAFNLF
                     LGDYGELVILPSLMQWLDGMQAKVRINLRAASSDQVADQLRDGRLDLWLTSEPIVDNS
                     FVNQPVGTERLVSMVRRDHPLVKDSLSLAQFLTLRHVMIEWHDPKGTVVEHALRARGI
                     ERQCGMQVHSFFDMPRVVSATDMICTLPSQMARHFTEAHNLKAYPLPELDIDIPMFLT
                     WHKRFDPDPGHTWIRDSIIRLLTSR"
BASE COUNT         1136 a         1338 c         1359 g         1104 t
ORIGIN      
        1 ggcctctagc ccttacagaa cctgcgcgag cagctattaa accaatagca accatcgcgc
       61 atcagcgata tcgctagcag acattgacgt tgagatcgcg ctgaactatg gatgacggct
      121 tacgggcagg gaattaaaac gtcgctatga ctgcagttgc gtcgacagcc gctgagggcc
      181 ctaaccggcc atagccccgc cagagtctga acaatcagac cgcagcgttt acaggtattc
      241 gccaccgtca gcttccaggc ttcacaattc attgtctatc agcttctttg gtagataaac
      301 ggaagacgct atgtgtacgg agtcgatgat gcaaattgca ggcatccccc agttatttgg
      361 accgacctaa aacagcagtt tggtcacagc caattcactg gcccagtatc cagatacttt
      421 gggcaaatca tcttcaaaat ccttcacccg cgagtgataa aagatatgaa cgctggttgg
      481 tggaagcaca ctttgatccg ggaagtttgc gctttgcaca aatgcgagcc gaacaaacgg
      541 ggccaaccgc aggaaaccca ccacaggggc accacacatg tcgcaagtag accgacgcag
      601 tgcgggtggc ggacggtatt tacgaaacgt aaaaggctgc acatccggca gcgtaatggc
      661 cccgccccac atcatgacta cgtctgcata gggtttcttg tacagcgtct gacatatagt
      721 gcaatggcac ttgaatcggc cgatgggagt cccatccact ttgaaaatgg actggccaca
      781 ttggcaggaa gcagtttgag tgagtcgctt gtaggtcata cctttggcac tgcaaattgt
      841 gttgaattga gttcggttga ctgcaacaac caatccatct cattcatggt tgtgtcttct
      901 cgccggtgat ggcctggttc agcaccaact cgccctcaaa tgtccattgg cgcccagtgg
      961 cactgacggg cgtgatgtag agcgtaccga tgccctgaag gccgacgagt tttccggtgc
     1021 tcccgaccac accccaatga ccgtcgaaca gattgcgcgc cttaccgtcc tggaccactt
     1081 ggatggcgtg ccactgatat ttcagataag ccttatcccc atttgccaaa gtgaagacaa
     1141 gatatccggc accgtccgca ccaccttggg ccggcacaat atcgtgcatg ccccactcct
     1201 gcatgattgc gctggcaagc ggacctgcgc cgctagtcat accctcacgc tgaaccaggg
     1261 atagggtacg gttgctgcca tcggcgaaca ccagtttggc ttccgccgtg ggggcgatga
     1321 cggaaacgaa cctgatccgc tcagcccaac ttggcgcagc gacagctcct gccaagagca
     1381 gtgtggcaac ctgatgactc aggttggaca aggatttctt caaacgcatg gtctttcctc
     1441 attgggtcat catggcggtc atggccgcca tgggtgccgt gttctacatc ttgatcgtga
     1501 ctcggccgtt ttggcttgtg taaatgcgtg attgcgcttc cgaatcggcc ttggtaggtg
     1561 ggcgcagatc agcaggctgt gcggtcttca gaacgggcca ccacaacagc acgtaccaag
     1621 gttccacctt cacggttttg ggcacgccga aggcctcttg ccgggtgtgg gggatgatgc
     1681 ccgtgcggtg aatgacatcg aaaatgaaca ggaacggtgc atagttggtc atgccgttgc
     1741 catatttgcc aatgccatgg tgcgcatggt gggtgtcctg caaggtgacc acgcgctcca
     1801 gcacccacat aaagggctga gtgaatctgt ttttgtgcaa cgccagatcc caacgcgacc
     1861 cgctatggga aaggaatccg acaatgatct tcgctgagcc cgccagcacc acgggggcaa
     1921 tatggccacc ccagatgaag aatgctgtga tataggtgtt ggatgccagt gcccaccaca
     1981 accagttctc gcggtaggtg accgtcacat tcatgtgctc gggtgtatgg tgggccttgt
     2041 ggatgcgcca cagccagggg atggagtgtg acaggcggtg aatccaatag tgcgcgtatt
     2101 cttcgaccag catgtagaca aagaaggtgg tccaaaagcc catgccgttc atgaatccag
     2161 tgcccccagg gaaggccttc aggaagatcc acacccaaac gcccaccatc agcgctctgg
     2221 cggtcaggaa attgacaaag gcaaagacgt tcaagcccat ctcttgcatg gtgtattgac
     2281 gcctgtcgct tagtgcgcct gacaggatgt cgatgaccag gaagaatatg agcgaatatg
     2341 caaatacgag catggcttga tgctctgtcc agttgtgcag gatgtcgatg atgtccacga
     2401 tggtctccgt tgttctctat gggttcaagt tgttaggctg caaccaaatc ctgtgcattg
     2461 gtggtcggat gcaatgcgct ttggtctagg aatttgtcga aatgcagccg gtctgccggg
     2521 atatgtccct tgaccttggc aatcaccgca tccaccatgg gaggcggacc gcagaggtaa
     2581 gcgtcatggt ctgcatagtt gggaaccacc tggaccacat ggtccgtgac cagacctcgc
     2641 ctgccggtcc agtttgaatt cacgggttca tgcgaaagaa ccgggtggaa cgcaaacgag
     2701 accggccacg cagcagccat ctcggcaatt tcatccaagc aatacaaatc acgctgctgg
     2761 cgggcgccaa acaaaaaaat cacctggcgt gaaatgccct cggacctcag ttgttccaac
     2821 agagccttga tgggtgccag gccgctgcct ccggccacgc agaggagtgg ccgttcggtt
     2881 ttgcgatagc ggaaatcacc gtaggggccc gtcaccgtta agcgtgcgcc cgtccgatca
     2941 gcggcaaaca gccaatcggt aaacagtcca cccggcacat ggcgaatata gaaagaagcg
     3001 gagttggcat tgccgactcc gggagcgctg gcaaatgaat agttgcgcgg tgtctctgcc
     3061 aactgtggct ctgcgggcac agacaactgc gcatattggc ctgcgatgta ggccaacggc
     3121 cgctccaagg ccacctgcac ctcgaaaatg tcgtgggtta gcgggatcag tgcactgatc
     3181 gtcgcgctac tggtgaccaa agagtcgtct tctgggctca acccaggaac ccggatcacc
     3241 aagtcacttt cggcaattgt ttggcaggca agtacgtagc cctgctcaat ttcttccaac
     3301 gaaatgtggt ttgtgatgtc acgcgtgagt cgaacgcgac catccataac gcgacagcgg
     3361 cacgtaccgc attcgccaac accacaatag ttcgggactt tgaatctggc atccaccgct
     3421 gcagtcaaca cggactgatt gggcttcatg ctggtcctga agttggtgcc ttcgatactc
     3481 agctggttgg cagctttgga tgaacccttg aataacgcct tgaacacttg catctcctga
     3541 tccccttatg ggcaggcgta atgtaggctt gacgagaaaa taataaaaat tgatattcaa
     3601 tatgcctgat atatgatgta ttgatatcga acctttgtac gagccagcga tgaacttacg
     3661 caaagtggat ttgaatctgc tgacggtgtt tgatgcagtc atgcgtgaag gcaacttcac
     3721 gcgggccgcc gacaaaattg gaatgaccca gcccgctgtt agtgaggcgg tggcaaggtt
     3781 gcgccacctg ttcaaggatg atttattcat ccgcaacgga cgcggtgttc agcccactcc
     3841 gcgcgcccat caatatgcag caccgatacg gcgaagtctc gatctaattg ccgcaacgct
     3901 ttcggaatcg cataccttcg atttcgtgac gagtacgcgt gcgttcaatc tgtttctggg
     3961 tgattatggg gaattggtga ttcttccaag tctgatgcaa tggttggatg gcatgcaagc
     4021 gaaggtgagg atcaacttgc gcgccgcttc cagcgatcaa gtagcagatc aattgcgcga
     4081 tgggcgattg gacctttggc tgacatcaga gccgattgtg gacaatagct tcgtcaatca
     4141 gcctgtcggg acagagcggt tggtgtctat ggtgcgccgc gatcatccgc ttgttaaaga
     4201 ctccttgagc ttggcgcaat tcctgactct gcgccacgtg atgattgaat ggcatgaccc
     4261 gaaaggtact gtagtggagc acgcactgcg ggcgcgcggc atagagcgtc agtgcggcat
     4321 gcaggtgcac agtttttttg acatgccccg ggtggtctca gcgactgaca tgatttgcac
     4381 attaccctcc caaatggcac ggcattttac cgaggcgcac aaccttaagg catatccttt
     4441 gccggagctt gacattgaca ttccgatgtt cttaacttgg cacaagcgct tcgacccgga
     4501 tcctggccat acttggatcc gggattccat catccgactg ctaaccagcc gttgagtgct
     4561 ccctccgtcg gtattacccg gcttcgtaag caccacgggc caatctgcca ccccaagcgc
     4621 cagtgtgtcc gtcagcgtct gcacgtgccg gccactgtgc aaacgggact tcaccaaagc
     4681 ggtcatacac gatgcgatta cgtaagctgg tgaatgtcgg ctgtcaggct atgaattgca
     4741 tgatcgctac gactgcaatg ggtcggcagc tcccagtcgc aatacccggc agcagacttt
     4801 caaatcaccg ctgaatgcct gagaatcgcc agcgtcagct tttcacctga tacccgccgt
     4861 tgattccgcc agggcgtttg cgttcgagtg actgttgtga ggatcagacc gtgaagtgac
     4921 ggtgcggtcg cgaacgg
//
LOCUS       DAOSLL010000346         4926 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15279_length_4926_cov_4.578526, whole genome shotgun sequence.
ACCESSION   DAOSLL010000346 DAOSLL010000000
VERSION     DAOSLL010000346.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15279_length_4926_cov_4.578526"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="PLB25_14310"
     CDS             complement(<1..522)
                     /locus_tag="PLB25_14310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOE42784.1"
                     /translation="MTTPQSLTSALDQQIAQHILQASGWIGFDVFMRQALYAPGLGYY
                     AHGSAKFGVLPYTVQDGIKAAGSDFVTAPEMTPLFGWSLAQQVAQALQVTGTAEVWEF
                     GAGSGALALQVLQALKAQGVPLQRYSIVDISGSLRSRQRATLHEFAAQVHWVDALPDS
                     LQGVVLGNEVLDAM"
     gene            543..1328
                     /locus_tag="PLB25_14315"
     CDS             543..1328
                     /locus_tag="PLB25_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE42785.1"
                     /translation="MSHEALPTNRPRTVLITGAAKRLGREIALTLAKEGWRVAVHFRD
                     SVEDASRTVADCKRLAGASASFRANLGNETAVRNLLPNVVSQFGHVDALVNSASTFEH
                     DTPETFSFAAMEKHMRSNAGAAILLSQALLEHLQGRGDAATGVVINLLDQKLWNQNPD
                     FTSYTLSKAALHAANTMLAIALAPRIRVVGVAPGLTLTSHILSPERFEMLHKLSPLGH
                     SSTAADVAATVSFALNNSSITGTTLLVDGGQHLMKFERDFSLM"
     gene            1387..1773
                     /locus_tag="PLB25_14320"
     CDS             1387..1773
                     /locus_tag="PLB25_14320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HOE42786.1"
                     /translation="MTLNLASHTHGDQTLTLTGLCFDANLGILEAEKNAPQPIQVDAE
                     LSLGAQPLLPRDDDITHVLDYRKVRKIIIDECTAEHVNLLETLIGKLANRLMRLPGVK
                     GVRVKIAKLEIFADCEVAIRMETGQW"
     gene            1838..2743
                     /gene="ttcA"
                     /locus_tag="PLB25_14325"
     CDS             1838..2743
                     /gene="ttcA"
                     /locus_tag="PLB25_14325"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HOE42787.1"
                     /translation="MPANAGGASQPKIARETLKLEKRLCRQVGQAIIDFNMIEEGDRV
                     MVCVSGGKDSFGLLDILLKLQKRAPVRFDIVAVNLDQKQPGFPAHILPDYLGKLGIEF
                     HIETQDTYSIVKKVVPEGKTMCSLCSRLRRGILYRVADELKISKIALGHHRDDMLQTF
                     FLNMFFAGKLKGMPPKLVSDDGGHIVIRPLAHVNEKDLIRWAAHRQFPLIPCTLCGSQ
                     ANLQRQQIGQMLRDWEKQYPGRTGTMFTALQNVVPSHLMDRQHYDFKNIQLTHQADAD
                     GDKAFDEDTFALASLPASNLVQLGF"
     gene            2795..3349
                     /locus_tag="PLB25_14330"
     CDS             2795..3349
                     /locus_tag="PLB25_14330"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HOE42788.1"
                     /translation="MIVTLIAGCAGVRLVDNQVSSFAPKPVATGASFRFERLPSQLAD
                     APAQQRVEAFTEQALAKVGLRRADHNADLIAQILVTERQAVVTGPFFGVGLGFRLGHH
                     GGIGHRRGSFFPAMGGQPNYWREVHLTLREQASMAVVFETRASHDGPWPDTDAILPAM
                     LDAALQGFPEPPPGVRQVNIEIPR"
     gene            3365..4009
                     /locus_tag="PLB25_14335"
     CDS             3365..4009
                     /locus_tag="PLB25_14335"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HOE42789.1"
                     /translation="MQVTRIIAIRHGETAWNVDTRLQGHLDIALNDVGLWQASRVALA
                     LTDEPIDTIYTSDLMRAWQTAQAIAEVAGCPVITDQGLRERGFGEFEGKTHAEIEADW
                     PDMSLQWRRREPDWAPPGGESLNTMRERVQSAASALAAQHIGGQIVLVAHGGVMDILY
                     RLATRQELQAPRAWNLSNAAINRLLWTPEGFSLVGWADTGHLEDSSSLDETVVT"
     gene            4653..>4926
                     /locus_tag="PLB25_14340"
     CDS             4653..>4926
                     /locus_tag="PLB25_14340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HOE42790.1"
                     /translation="MQSHSTARTLYDKLWDEHVVHTEDDGTALLYIDRHLVHEVTSPQ
                     AFEGLRQTDRKVWRVSSIVATADHNTPTTGWERGYDGMTDPTSKEQV"
BASE COUNT         1039 a         1546 c         1429 g          912 t
ORIGIN      
        1 catcgcatcg agcacctcat tgcccagcac cacgccttgc aaactgtcgg gcagcgcatc
       61 gacccagtgc acctgtgcgg caaattcatg caaggtggcg cgctgacgcg agcgcaagct
      121 gcccgaaatg tccacaatgc tgtagcgctg cagcggcacg ccctgtgctt tgagcgcttg
      181 cagtacctgc agcgccagcg cgccggagcc agcaccaaac tcccaaactt ctgccgtgcc
      241 ggtgacttgt agcgcttgcg ccacctgttg cgccagactc cagccaaaca gcggggtcat
      301 ctcgggggcg gtgacaaagt cgctgccggc ggccttgatg ccgtcttgca cggtgtaggg
      361 caaaacgcca aatttggcac tgccgtgggc gtagtagccc aaacccggcg cataaagcgc
      421 ctggcgcatg aaaacgtcaa aaccgatcca gcccgaagcc tgcaggatgt gctgggcgat
      481 ctgctggtca agcgcgctcg ttaaactctg gggtgttgtc atagcctttg gattgtcccc
      541 gaatgtcgca cgaagcctta cccacaaacc gaccgcgtac tgttttgatc accggtgccg
      601 ccaaacgcct gggccgcgaa attgcgctga cgctggccaa agaaggttgg cgcgttgccg
      661 tgcattttcg tgactcggtc gaagatgctt caagaacagt agctgactgc aaaagactgg
      721 cgggggctag cgcctctttt cgtgccaatc tgggaaacga gacagcggtg cgcaacctgc
      781 tgcccaacgt ggtttcccag tttggtcatg tcgatgcgct ggtcaacagc gcctccacct
      841 ttgaacatga cacacccgaa accttcagtt ttgcggcgat ggaaaagcac atgcgcagca
      901 atgccggggc ggcgattttg ctcagccagg cgctgcttga gcacctgcaa ggccggggtg
      961 acgcagcaac tggcgtggtg atcaatctgc tcgaccagaa gctgtggaac caaaaccccg
     1021 acttcaccag ttacaccttg tcgaaagcgg cactccacgc cgccaacacc atgctggcga
     1081 ttgcgctggc accgcgcatc cgggtggtcg gcgtggcccc tggcctgacg ctgaccagcc
     1141 atatcctcag cccggaaagg tttgaaatgc tgcacaagct gtcaccgctg ggacactcgt
     1201 caaccgctgc cgatgtggct gccaccgtgt catttgcgct gaacaacagc tcgatcaccg
     1261 gcaccacgct gctggtcgat ggtggccagc acctgatgaa attcgagcgc gatttttcgc
     1321 tgatgtaagc cgccgcccgg ttttcgccac ctgttgttat ttcatcgtga ttgattgacc
     1381 caagccatga cattgaacct tgccagccac acccacggcg accagaccct aaccctgacc
     1441 ggcttgtgtt ttgatgccaa cctgggcatt ctggaggctg aaaaaaatgc gccgcagcca
     1501 atccaggtcg atgccgaact gagcctgggc gcgcagccgc tgctgccgcg cgacgacgac
     1561 atcacccatg tgctggacta ccgcaaggtg cgcaaaatca tcattgacga atgcaccgca
     1621 gaacacgtca acctgctcga aaccctgatt ggcaagctgg ccaatcggct gatgcgtctg
     1681 cccggtgtca agggcgtgcg ggtcaagatt gccaagctgg aaatttttgc cgactgcgaa
     1741 gtcgccattc gcatggaaac cggccagtgg tgaccaggtc atgatcatga atgcctggat
     1801 cgacccgagc gacactgaac tggccagcgc gccagcaatg ccagccaatg caggcggcgc
     1861 aagtcaaccg aaaatcgcgc gtgaaaccct caaactggaa aaacgcctgt gccgccaggt
     1921 tggccaggcc atcatcgact tcaacatgat cgaagagggt gaccgtgtca tggtgtgtgt
     1981 cagcggcggc aaagacagct ttggcctgct tgacatcctg ctgaaattgc aaaagcgcgc
     2041 gccagtgcgc tttgacatcg tcgctgtcaa cctcgaccag aaacagccag gctttccggc
     2101 ccacatcctg cctgactatc tgggcaagct gggcattgaa ttccacatcg aaacccagga
     2161 cacctacagc atcgtcaaaa aagtggtgcc cgagggcaaa accatgtgca gcctgtgcag
     2221 ccgcctgcgc cggggcatct tgtaccgggt ggccgatgag ctcaagatca gcaagatcgc
     2281 gctggggcat caccgcgacg acatgctgca gacttttttc ctcaacatgt tttttgccgg
     2341 caagctcaag ggcatgccgc ccaaactggt cagcgacgac ggtggccaca tcgtgattcg
     2401 cccgctggcc catgtgaacg agaaagacct gatccgctgg gccgcgcacc ggcagtttcc
     2461 gctgatcccc tgcacgctgt gtggcagcca ggcgaacctg cagcgccagc agattggcca
     2521 gatgctgcgc gactgggaaa aacaataccc tgggcgcacc gggaccatgt tcaccgcact
     2581 gcaaaacgtc gtgccctcgc acctgatgga ccgccagcat tacgacttca aaaacatcca
     2641 actcacccat caggccgatg cagacggcga caaggccttt gacgaagaca cgttcgcgct
     2701 tgccagcttg cccgcaagca acctggtgca gcttgggttc taagcacaaa atagccctct
     2761 agcccttatg aataaagcgc tgatagctct gtttatgata gtaaccttga tcgctggctg
     2821 cgccggtgtg cggctggttg acaaccaggt cagcagcttt gcgcctaagc cggtggcaac
     2881 cggtgccagt ttccgctttg agcgcctgcc ctctcaactt gccgatgccc cagcccagca
     2941 acgcgtggag gcgtttaccg agcaggcgct ggccaaagtc ggcctcaggc gcgctgacca
     3001 caatgccgat ttgatcgcac agatcttggt aaccgagcgc caagcggtcg tgactgggcc
     3061 gttctttggc gtgggcttgg gtttccggtt gggccaccac ggcggcatcg gccaccgtcg
     3121 tggtagcttt tttcccgcga tgggcggtca acccaattac tggcgcgaag tccacctgac
     3181 cttgcgcgaa caggccagca tggccgtggt gttcgagacc cgcgccagcc atgatggccc
     3241 ctggcctgat accgatgcga tcctgcctgc catgctcgac gctgccctgc agggctttcc
     3301 cgagccaccg cccggtgtgc gtcaggtaaa tatcgaaata ccgcgttaaa caggacagtc
     3361 cgccatgcaa gttacccgca tcatcgccat ccgccacggc gaaaccgcct ggaatgtgga
     3421 cacccgcctg caagggcatc tggacatcgc gctcaacgat gtgggtctgt ggcaggccag
     3481 tcgggttgcg ctggcgctga ctgacgagcc gattgatacc atttacacca gcgacctgat
     3541 gcgcgcctgg caaaccgctc aggcgattgc tgaagtggca ggttgcccgg tgatcaccga
     3601 ccagggtttg cgtgaacgcg gctttggcga atttgaaggc aaaacccatg ccgagattga
     3661 agccgactgg cccgacatgt cattgcaatg gcgcaggcgc gagccagact gggcgccgcc
     3721 gggtggtgaa tcgctcaaca ccatgcgcga acgggtgcaa agcgccgcat cggcgctggc
     3781 cgcgcaacac atcggtggcc aaatcgtgct ggtggcccat ggtggcgtga tggacatcct
     3841 gtaccggctg gccaccaggc aggaactgca agccccacgc gcctggaacc tgagtaacgc
     3901 cgccatcaac cggctgctct ggacccccga gggcttttcg ctggtcggct gggccgatac
     3961 cgggcatctg gaagacagca gcagcctcga cgaaaccgtt gtgacctgaa tgccgccggg
     4021 cgtaagggcc agcttggcga aggccagtgc cgggcggcgc cctggttccc atcaagacgc
     4081 agcttgatga tgcccacaaa gcaggtcagt ctgatgccgg tgacaaacag ttcgctgacc
     4141 caggcagcag gccaagttaa tgatgcaaat tggcctctag cccccgtcct tattgcgctg
     4201 acagctattg tttttgagat tattcaggat ggccgctgag cacctggcga agggtgctgc
     4261 caatcaatca agcggcaatc gggtgcattg catcggtgcc gcttggcatc gcgtctgtct
     4321 gggtgggttc gaaacagatg ccacccactt cggtcaacat gcgggtcgct gcgggtggta
     4381 tcaggcatgc tggcacaagc tccggtgaac caacaggcag accggcaaca tctcaccggg
     4441 aagggctagc cgccttgccc atgtcagcaa ccctgccgac ctcgcgcttc atggcaagac
     4501 gcacgccagg gcagggcgct tgcccaaagg caatgccagg gcacaccggc cgcgccaggt
     4561 tggcgcatgc caggccggcc ggttgctcac ccatcaaggt ctggcctgcg ccgtttaaaa
     4621 tggctggatt gcctcaatca cggatggtcc gcatgcaatc acactcaact gcccgcacgc
     4681 tgtacgacaa gctctgggac gaacacgtcg tccacaccga agacgatggc accgcgctgc
     4741 tctacattga ccgccatctg gtgcacgaag tcaccagtcc gcaggccttt gagggcttgc
     4801 gccagaccga ccgcaaagtc tggcgcgtca gttcgattgt tgcaaccgcc gaccacaaca
     4861 cccccaccac cggctgggag cgtggctacg atggcatgac cgaccccacc agcaaggaac
     4921 aggtgc
//
LOCUS       DAOSLL010000347         4921 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15298_length_4921_cov_3.780723, whole genome shotgun sequence.
ACCESSION   DAOSLL010000347 DAOSLL010000000
VERSION     DAOSLL010000347.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4921)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4921)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4921
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15298_length_4921_cov_3.780723"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="PLB25_14345"
     CDS             <1..1335
                     /locus_tag="PLB25_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE42791.1"
                     /translation="PDGIAKAINEVDENIVAFNKVWGEYMATYLTPDEAQNAKSFAEA
                     HSLLIQDGLKPTMAALKSGKTEEAARLVRDKIRPIVTNAGKYISALFKIQFDVSAQEF
                     KAATARFELIRLVVIGATLAGLVFACWFGLALMRSISVSLNKIIEISNAVAQGDLTHT
                     IQISGKDETVELMTSIATMQNKLIDLVSHVRQGSEGVATASAEIAQGNHDLSARTESQ
                     ASALEETAASMEELSATVKQNAAAAVQANQLARNASTVAIQGGEVVGQVVETMKGINE
                     SSRKISDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAGRSAEAA
                     KEIKDLISTSVERVEQGTALVDKAGTTMTEVVSSIQRVTDIVAEISAASNEQNSGVSQ
                     IGEAVTQIDSVTQQNAALVEEMAAAASSLNTLAGEQVQSVSVFLLPSNARLA"
     gene            1403..4486
                     /locus_tag="PLB25_14350"
     CDS             1403..4486
                     /locus_tag="PLB25_14350"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE42792.1"
                     /translation="MDWLRRFKFGQRLQLLIVIFSLGFVAYGVWTYQTLQIAKVGGPL
                     YSQIERNQELVSDVLPPPANIIEAYLTCIEIISANDSYRQGALIDRLKQLQQDYLERH
                     QYWKQANLDPPLAKALLEQAHEPAMAFFTVTFRNFLPAVFLNDRLGMERVLRDMTKQY
                     KVHRLAVDSVVVMARQNAHSNKLAAIAQVESARLWQLGIFIFSLAVAIGFALMIQRSI
                     LKPLTQAIDIAKAVASGDYQVPQEQHYPDEAATLLRTLREMGDSLQRAMAALQDATRF
                     AEAQSRSRSEFLANMSHEIRTPMNAIIGMTRLALRTDLTSKQRNYLSKVSAAAQGLLG
                     IINDVLDFSKIESHKLHFEAKTFKLDHTLDHLAALTVAKAHLKGLELLFEVDPTVPGA
                     LVGDELRLGQVLLNLVSNAIKFTATGEIRLNVRCLEQHATQVRLQFEVHDTGIGIAPE
                     HSAKLFAPFQQADSSTTRKYGGTGLGLTIARQLVEMMGGTIWLESQPGVGSRFVFTAL
                     LGLPDESPMELPSSDIQLKGQRALVVDDNSSARRIMCEILESLHLKAQAVSSGPDAIA
                     ALEEAQQASRPFNLVLMDWQMPGMDGLETLRQMRDLEGVTDLPATIMVTAYDRDALQE
                     SAREFDLSGILEKPVNPSNVLDALMRAVSHQRESQLAAQTLASQPTQTVDLRGARILL
                     VEDNDINQELAAELLTEAGGVVEIANNGAEAVDMVRRTAFDAVLMDWQMPVMDGFEAT
                     RIIRADARFAQLPILAMTANVMNGDREKCLAVGMNDHISKPIEVGQMLETLSRWINST
                     RIQPGTALIASDATDAPVQPELPGVDVAGAMHRMGDSLPRYHKLLERFIQNQADAATV
                     IREALALGAHEDARRGAHTLKSLAATIGASQLAHDAQALEHAAHSGALDQCSHLVDQV
                     ETQLHPLLVSIRGYLETRREPAEQTAQELNPMAGQETAVSDSTLQSIEQLHRLLSQDD
                     SQAIKLLAPIASALKGHPAADAFERVIAAAKDYDFVTALTELTTTERILVPQPQP"
     gene            4495..>4921
                     /locus_tag="PLB25_14355"
     CDS             4495..>4921
                     /locus_tag="PLB25_14355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE42793.1"
                     /translation="MQSDTLTQNLHRKHTVLVVDDAPENIDQLCDLLEQEYVVKIATQ
                     GKRALQIVMSDQPPDLILLDVLMPGISGFEVCRQIKNNPARRNIPILFVTSLEQGDDE
                     AMGLSLGAEDYIVKPFRPPLVMARVRTHLALFNQKQELER"
BASE COUNT         1152 a         1369 c         1387 g         1013 t
ORIGIN      
        1 cctgacggta tcgccaaagc cattaacgaa gttgacgaga acattgttgc gttcaataaa
       61 gtgtggggcg aatacatggc aacctacctc acgccagatg aggcacagaa cgccaaaagt
      121 tttgccgagg cacacagctt gctcattcag gatggactaa agcccaccat ggccgccttg
      181 aaatctggca aaactgagga ggctgcccgc ctggttcggg acaagattcg accgattgtc
      241 acgaatgctg gcaagtacat aagcgccttg ttcaagattc agtttgatgt ttcggcacag
      301 gagttcaaag ctgccactgc acgttttgag cttatccgat tggttgttat cggtgcaaca
      361 ctggcaggtc ttgtgtttgc ttgctggttt ggcctggccc tcatgcgctc aatcagcgta
      421 tcactgaaca aaatcatcga gatctccaac gctgttgcgc aaggtgatct cacccacacc
      481 atccagatca gtggcaagga tgaaactgtg gaactgatga catcaatcgc caccatgcag
      541 aacaagctga ttgatctggt ttcccatgtt cgacagggtt cagagggggt ggcaacggcc
      601 agcgcagaaa tcgcccaagg caaccatgat ttgtccgcgc gtaccgagtc gcaggccagc
      661 gcccttgagg agactgctgc gagcatggaa gagctcagtg ccaccgtcaa gcaaaacgcc
      721 gctgctgcgg tacaggccaa tcaattggca agaaacgcct cgaccgtcgc aatacaggga
      781 ggcgaggttg ttggccaggt ggttgagacc atgaagggga tcaatgagtc tagccggaag
      841 atctcagaca tcatcagcgt cattgatggc atcgcttttc aaaccaatat cctggcgttg
      901 aatgctgcgg ttgaagccgc acgagcgggc gaacaagggc gcggatttgc cgttgtggca
      961 tccgaggtgc gttcacttgc aggccgtagc gccgaagctg caaaggaaat caaggacttg
     1021 atcagcacca gtgttgagcg agtcgagcaa ggcactgcat tggtggacaa ggctggcacc
     1081 accatgaccg aggtggttag cagcattcag cgggtgactg atatcgtggc tgagatatct
     1141 gctgcaagca acgagcagaa ttcaggggta agccagattg gtgaggccgt gacccagatt
     1201 gattcagtca cccaacagaa tgcggctctg gtggaagaga tggccgccgc agccagcagc
     1261 ctcaacacac tggctggcga gcaggtgcaa tcggtttcgg tgttcctgct gccatcgaat
     1321 gcccggctgg cttagatcag acaggcccgc tggctgagcc attggtcaat cagcccgaca
     1381 gtcaacaacg gagtcgatac aaatggactg gctacgccgc ttcaaatttg gccaacgatt
     1441 gcaattgctg atcgtcattt tttctctggg gtttgtggcc tatggtgtct ggacctatca
     1501 gacgctgcaa atcgccaagg tgggtggccc gctgtacagt caaattgagc gcaatcaaga
     1561 gttggtttct gatgtgttgc caccacccgc caacatcatt gaggcgtacc tgacctgcat
     1621 cgagatcatc tcggccaatg acagctaccg gcagggtgct ctcattgatc ggctcaagca
     1681 gttacagcaa gactatctgg aacgccacca gtattggaag caggccaacc tggacccccc
     1741 tctggcgaag gcgctgcttg aacaggctca cgagccggcc atggcgtttt tcaccgtgac
     1801 gttccggaat ttccttccag cggtcttttt gaatgaccgc cttggcatgg aacgggtcct
     1861 tcgagacatg acgaaacagt acaaagtgca ccgcctggcc gttgatagtg ttgttgtgat
     1921 ggccagacaa aacgcccatt ccaacaaact cgctgcgatt gcgcaggtgg aatccgcaag
     1981 gctttggcaa cttggcatat tcattttctc actggccgtt gctatcggtt ttgcgttgat
     2041 gattcaacgc agcattctga agcccctaac ccaggccatt gatatcgcga aggcagttgc
     2101 ttctggtgat taccaggtgc cgcaagagca gcattacccg gacgaagccg caaccttgct
     2161 gcgcactttg cgcgaaatgg gcgacagtct gcaaagggca atggcagcgc tgcaagatgc
     2221 cacgcggttc gccgaagccc aaagccgcag cagaagtgaa tttctggcca atatgagcca
     2281 cgagattcgc acccccatga acgccatcat tggcatgacc cgcttggcct tgcgaaccga
     2341 tctgacatcc aagcagcgca attacctcag taaggtgagt gcagcagccc agggtctgtt
     2401 aggcatcatc aacgatgtac tggacttctc gaaaattgag tcccacaaac tgcattttga
     2461 agcaaagact ttcaaactgg accataccct ggatcacctg gcggcgctga ccgttgccaa
     2521 ggcgcacttg aaagggcttg aattgctgtt tgaagtagat ccgactgtac ccggcgcgct
     2581 ggttggcgat gaattgcgtc taggccaggt cctgctgaat ctggtcagca acgccatcaa
     2641 attcacggca actggggaga tacggttgaa tgtgcgttgc ctggagcagc acgccactca
     2701 agtgcgcttg caattcgaag tacacgacac cggtatcgga attgctcccg aacacagtgc
     2761 caaactgttt gcgcccttcc agcaggcgga ttcatcgacc acacgcaagt atggcggcac
     2821 tggtctggga ctgaccattg cacgccaatt ggtggagatg atgggtggaa ccatctggct
     2881 ggaaagccag ccaggtgtcg gtagccgctt tgtattcacc gcactactgg gactgcccga
     2941 tgaatcgcca atggaactgc cctcgtcgga cattcagctc aagggtcagc gtgctctggt
     3001 ggtcgacgac aacagcagtg cccgcagaat catgtgcgag attctggagt cgctgcattt
     3061 gaaggcgcaa gctgtctcta gcggcccaga tgccattgcg gcacttgaag aggcacagca
     3121 agcatcgcgc ccatttaatc tggtgctgat ggactggcaa atgcccggca tggatggtct
     3181 ggagaccttg cgtcagatgc gcgacctgga aggagtaacc gacctacccg ccaccatcat
     3241 ggtcaccgcg tacgaccgtg atgccctgca agaaagtgcc agggaattcg acctgagcgg
     3301 cattctggaa aagcccgtca acccgtcgaa tgtgctggat gccttgatgc gcgcggtgtc
     3361 gcatcagcgg gaaagtcagc ttgcagcgca aaccttggcc agccagccga cgcaaacagt
     3421 cgatctgcgc ggagcccgca tcttgctggt agaagacaac gatatcaatc aggaattggc
     3481 tgccgagctc ctgaccgagg caggtggggt ggttgaaatc gccaacaatg gcgctgaagc
     3541 ggttgacatg gtccgccgga ccgcatttga tgccgtcctg atggactggc agatgccggt
     3601 tatggatggt ttcgaggcta cccggatcat ccgtgccgat gcccgctttg cacaactgcc
     3661 gattctggcg atgaccgcca acgtcatgaa cggcgaccgc gagaagtgcc tggctgtcgg
     3721 catgaatgac catatctcaa agcccatcga ggtgggccaa atgcttgaaa cgctgtcgcg
     3781 ctggataaac tcgacgcgca ttcaacctgg cacagcactg atcgcctcgg atgccacaga
     3841 tgcccctgtc caacccgagc tacctggcgt ggatgttgcc ggggcaatgc accggatggg
     3901 cgatagcttg ccccgctacc acaagctgct ggagcggttt atacagaacc aggccgatgc
     3961 cgcgaccgtc attcgtgagg cgctggccct gggtgcgcat gaagacgctc gccgcggcgc
     4021 gcacactctc aaaagcctcg ctgcaaccat cggtgccagt caactcgcac acgatgcaca
     4081 ggcactggaa cacgcggccc acagcggcgc actcgatcag tgcagtcatc tggtcgatca
     4141 ggtagaaacg caattgcacc ctctgctggt atccatccgg ggttatcttg agacacggcg
     4201 tgagcccgca gagcagacgg cgcaagaatt gaacccgatg gctggtcaag aaactgccgt
     4261 ttctgacagc accctgcagt ccatcgagca gttgcacagg ctgttgagcc aggacgacag
     4321 tcaggccatc aaactgcttg ctccgattgc cagcgcactc aaagggcacc cggcggccga
     4381 cgcgtttgag cgagtgatcg cagccgccaa ggactacgac tttgtgacgg cactgacaga
     4441 attgaccacc actgagcgaa ttctggtacc ccaaccacag ccctagaggc gaccatgcaa
     4501 tccgataccc tgacccaaaa ccttcaccgc aaacatacag tgcttgtggt ggatgacgca
     4561 cccgaaaaca tcgaccaact gtgtgacctt ctcgagcagg aatacgttgt aaagatcgcg
     4621 acgcagggca agcgcgcgtt gcaaattgtg atgtccgacc agccgcccga cctgattttg
     4681 ctcgatgtgt tgatgcctgg catcagtgga tttgaagttt gccgtcagat caagaacaac
     4741 cctgcgcgtc gcaacattcc gattctgttt gtcacctcgc ttgagcaggg cgatgacgaa
     4801 gccatggggc tgtcacttgg cgctgaagac tacattgtca agccgtttcg cccgcccctt
     4861 gtcatggccc gtgttcgtac ccatctggcc ctgtttaacc agaagcagga actggagcgc
     4921 a
//
LOCUS       DAOSLL010000348         4904 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15372_length_4904_cov_5.990720, whole genome shotgun sequence.
ACCESSION   DAOSLL010000348 DAOSLL010000000
VERSION     DAOSLL010000348.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4904
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15372_length_4904_cov_5.990720"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="PLB25_14360"
     CDS             <1..197
                     /locus_tag="PLB25_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
                     /protein_id="HOE42794.1"
                     /translation="VMRVGADNRVSQLKVVTGRVLGDQVEIMAGLQPEDRLVTSGAGF
                     LSEGDVVHVVDAAKEKATLR"
     gene            complement(528..998)
                     /locus_tag="PLB25_14365"
     CDS             complement(528..998)
                     /locus_tag="PLB25_14365"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4143 domain-containing protein"
                     /protein_id="HOE42795.1"
                     /translation="MVFLLQPHHRNFGKRLVKSPKLYFHDTGLAAYLMGIADAQHLGI
                     HCARGALFENWVISELLKQRYNQGLPSNLFFWRNNTGEEVDVVVEQGDQLMPIEIKSG
                     QTFNADFLSGLSKWAGYAGAAALPAHLVYGGAVSMTRSGVAVHAWRELAGLLGL"
     gene            complement(1138..1692)
                     /locus_tag="PLB25_14370"
     CDS             complement(1138..1692)
                     /locus_tag="PLB25_14370"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE42796.1"
                     /translation="MIPRQATTTLQSLAKGYPILAVTGPRQSGKTTLAQSTFPDKPYV
                     SLEDLDTRAFATEDPRGFLARYPIGVILDQAQRCPALFSYLQTQVDADRRMGEFVLTG
                     SQQFGLPSNITQTLAGRVGLLQLLPFSWQDLQQGGAAVSSLDDLLWRGLYPPLYDRDL
                     APTQWFANYVMSYVERDVRQLIEV"
     gene            complement(1849..3336)
                     /locus_tag="PLB25_14375"
     CDS             complement(1849..3336)
                     /locus_tag="PLB25_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011204943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HOE42797.1"
                     /translation="MKPANPAQSVSASQAAPGDSPAMSGQGTLVDQLVQWAELRMAQQ
                     VFRPGMRMPSVRQLALERGVSRFTVVEAYERLVARGRLQARRGAGFFVREPKPAASSA
                     KRSRATPARAQTIDMGWLVRNMQSGIAADKAPGFGYLPPELCGGDLVKAGLRSVAATA
                     GLQLSHAAQAQGYAPLREQIARRLAELEVSATPAQILTTSGATQAVDLIARKFLRPGD
                     SVIVGNPAWSAQLGSLAMMGVNLVSLPYTPQGPDVSALAGLAASCKPVMLILNTVLHN
                     PTGTVLTAAAAYQVLRVAEAHDLIVVEDDIYADFLPSGLPAARLASLDQLKRVIYLGS
                     FSKMLVPNVRVGFLATSADIAEALGNQKLLTSLATPEINERIVHRALTEGSYRKHCQR
                     VHAALDELRDPTFARLESLGLQPYCRPQAGFQGWFDTGVDTVVLAALALEAGYLLAPG
                     ALFSPQQTPSTFMRMNIATSQNPALLKWLETTLKTLHRGAESAAL"
     gene            3488..3832
                     /locus_tag="PLB25_14380"
     CDS             3488..3832
                     /locus_tag="PLB25_14380"
                     /inference="COORDINATES: protein motif:HMM:NF022592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2917 domain-containing protein"
                     /protein_id="HOE42798.1"
                     /translation="MHQIEHFELATPHPVPLKLGAGCTIRVTHGRLWLTRPDHLEDVW
                     LHSGESWTLPAAGSVWLSAEPAAQFQLAHKLSGWRWPALANLRQRLPMLVNVSARLRG
                     LAHGAGSQLQLG"
     gene            4086..4571
                     /locus_tag="PLB25_14385"
     CDS             4086..4571
                     /locus_tag="PLB25_14385"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE42799.1"
                     /translation="MNIHEATAQDAATLALLISASNQDVAARFGLTLDNCPKHPSFCN
                     ADWIRADLARGERYFLAEQASGPMACVAFELAGAGVGYLNRLSVLPAHRRCGVGARLV
                     QHVLALAQSSGLERVSIGVIGQHMALQRWYSKLGFVTGDTQRFAHLPFSVTYMSCVVR
                     G"
     gene            4638..4826
                     /locus_tag="PLB25_14390"
     CDS             4638..4826
                     /locus_tag="PLB25_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42800.1"
                     /translation="MNSHTACAFGERRYQAWSEQVNTRYGGCVQKVFVANLPKGAQIR
                     KLFVPIGVDCRPPCGTRL"
BASE COUNT          962 a         1510 c         1508 g          924 t
ORIGIN      
        1 atgtgatgcg ggttggcgca gacaatcggg tcagccagct caaggtggtc accggccggg
       61 tgctgggcga tcaggtggaa atcatggctg gcctgcagcc cgaagaccgg ctggtgacca
      121 gcggtgccgg ttttctgagc gaaggcgatg ttgtgcacgt tgtcgatgct gccaaagaaa
      181 aagccacgct gcggtgattg atcagcgtcc atgatgctgg acgcctcagt tgaccgcttg
      241 ttttcgcctg tcatgccatt tccagatctt ccgtgtctag gagcctgtct gacttatggt
      301 acatcggctg caaatcgacc gcagcggtcc attttgcacc ggctttttga cccatagcgg
      361 ggctaggggc ctgcaaatcc ggcgcaaact gtgctcgctg ggggtcgatt ttcgctatcg
      421 atgccataag tccgacaggc tcctggaccc tgccgcagac aatgcaaacg caaggcaagg
      481 cgagataaca acgacatgga tgattcagaa atggcatgat tcgtgggcta caggcccaaa
      541 agccctgcca gctcgcgcca ggcgtgaacc gccacaccac tgcgcgtcat gctgaccgca
      601 ccaccgtaca ccaggtgagc cggcaacgcc gcggcacccg cgtaacctgc ccacttgctc
      661 aggccgctca gaaagtcagc gttgaaggtc tgcccggact tgatttctat cggcatcaac
      721 tgatcgccct gctccaccac cacgtccacc tcttcaccgg tgttgttgcg ccagaagaag
      781 aggttcgacg gcaggccctg gttgtaacgc tgcttgagca attcgctgat gacccagttt
      841 tcaaacagcg cgccgcgtgc gcagtgaatg ccgaggtgct gcgcatcggc aatgcccatc
      901 aggtacgcgg ccagaccggt gtcatgaaaa tacagcttgg gtgacttgac cagccgcttg
      961 ccaaagttgc ggtggtgcgg ctgcagcaga aacaccacat agcccgcctc cagcaccgac
     1021 agccaggcgg caacggttat gcgtcacgcc gcaatcgttg gccagcgccg tcagttgagc
     1081 aattgcccgc agcgcgccgc gcacatcttc aaaaaagcgc tggaacaagc tctggtttta
     1141 cacctcaatg agttggcgca catcacgctc cacataactc atgacgtagt tggcaaacca
     1201 ctgcgttggt gccagatcgc ggtcatacag cggtgggtac aaaccacgcc acagcaaatc
     1261 atccagactg ctcaccgccg cgccgccctg ctgcaggtcc tgccatgaaa acggcagcaa
     1321 ctgcagcaga ccgacgcgcc cggccagtgt ctgggtgatg ttggacggca ggccaaactg
     1381 ttgtgatccg gtcagcacaa actcgcccat gcgccgatca gcatcgactt gggtttgcag
     1441 gtacgaaaac agcgccgggc agcgttgtgc ctggtccaga atcacgccga tgggatagcg
     1501 cgccaggaaa ccgcgcgggt cttcggtggc aaacgcccgt gtgtcgagat cttccagcga
     1561 gacatagggc ttatctggaa acgtgctctg tgccagcgtg gttttgccgg actggcgcgg
     1621 gccggtcacc gccagaatgg ggtaaccctt ggcgaggctt tgcaaggtgg tggttgcttg
     1681 acgggggatc actttttaca aattggtaat ttagttccca aattgcaaaa acccgttggt
     1741 gtgacgagtt ggtgtgacgg accagcccgg cacgcatcgc agcaatacca ccacctatcc
     1801 cggccgaaaa caattcccgc ttcaaagcga cgcagtgggc agcaacgcct ataacgcagc
     1861 ggactcagcg ccccggtgca gcgtcttgag tgtggtctcc agccacttca gcagcgccgg
     1921 gttctggctg gtggcgatgt tcatgcgcat gaaggtgctg ggtgtttgct gtggcgaaaa
     1981 cagcgcgccg ggggccagca gataaccagc ctctaacgcc agtgccgcca gcacgacagt
     2041 gtccacgccg gtatcaaacc agccctgaaa acccgcctgt ggtcggcagt aaggttggag
     2101 accgagcgac tccagccggg caaaggttgg gtcgcgcagt tcgtccagcg cggcatgcac
     2161 acgctggcag tgtttgcggt agctgccttc ggtaagcgcc cggtgcacga tgcgttcgtt
     2221 gatttctggc gtggccagcg aagtcagcaa cttctggttg cccagcgcct cggcaatgtc
     2281 cgccgaggtg gccaaaaagc cgacgcgcac gttgggcacc agcattttgg aaaagctgcc
     2341 caggtaaatc acccgcttga gctggtccaa gctggccagc cgagccgcgg gcagaccgct
     2401 gggcaaaaag tcggcataga tgtcgtcttc gaccacgatc aggtcgtgcg cttcggcgac
     2461 ccgcaagacc tggtaggccg ccgctgcggt cagcacggtg ccggtggggt tgtgcaacac
     2521 ggtgttgaga atcagcatga cgggtttgca actggccgcc aggcccgcca gcgccgacac
     2581 gtccggcccc tgcggggtgt agggcaggct gaccaggttg acacccatca ttgccagcga
     2641 gcccagttgc gccgaccagg ccgggttgcc aacgatgacg ctgtcaccgg ggcgcagaaa
     2701 cttgcgcgca atcaggtcca ccgcctgggt ggcacccgag gtggtcagaa tctgtgccgg
     2761 tgtggcagac acttccagct cggccaggcg acgcgcaatc tgctcgcgca aaggcgcgta
     2821 gccctgcgcc tgcgccgcgt gcgagagctg caaacccgcc gtggccgcca ccgagcgcaa
     2881 gccggccttg accagatcgc caccgcacaa ctcaggcggc aggtagccaa agccgggcgc
     2941 tttgtctgcc gcaatgccgc tttgcatgtt gcgcaccagc cagcccatgt cgatggtctg
     3001 cgcacgcgcc ggggtggcgc ggctacgttt ggccgaactg gcagcgggct tgggctcacg
     3061 cacgaaaaac ccggcacccc ggcgcgcctg cagccgcccg cgcgccacca gccgctcata
     3121 ggcctccacc acggtaaagc gcgatacacc gcgttccagc gccagttggc gcaccgaggg
     3181 catgcgcatg ccggggcgaa acacctgctg cgccatgcgc aactcggccc actgcaccaa
     3241 ctggtccacc agcgtgccct ggccgctcat ggcagggctg tcaccggggg cagcttgtga
     3301 ggccgacacg gattgggctg gattggctgg tttcatggca gatgattgta ttggcggttt
     3361 gaccgggcca gtgtcaataa gtatccaggt cactgtaccg gtactgtact ggaatagaac
     3421 gtacatttac cagcccggta tcgcaaacct gtgacccact tgatcccacg taccaaagga
     3481 gcctgtcatg caccagatcg aacactttga attggccaca cctcacccgg tgcccctgaa
     3541 gctgggcgct ggctgcacca ttcgagtcac ccacgggcgg ctgtggctga cacggccaga
     3601 tcaccttgag gacgtgtggc tgcacagcgg tgaaagctgg acgctgccag cagcaggcag
     3661 cgtatggctc agcgccgaac cggcagcgca gtttcaactg gcgcacaagc tgtccggctg
     3721 gcgctggccc gcgctggcca acttgcgcca gcgcctgccg atgctggtga atgtgagcgc
     3781 gcgtctgcgt ggcttggcgc atggcgcggg ctcgcagttg cagcttggat agtcttgcgg
     3841 actcattcat tgctacacaa ctaatagctg tctgcgcacg cattgattgg gctacaggcc
     3901 taaaacacct aagccggccg agccggaatc gatgagcctt cgtcgcgagg gcgcgcctcc
     3961 tacacaaaca gtcggcgcca atttgaattt gtaggagtcg cgccctcgcg acgaatgact
     4021 ttgaatgttt gaattcttcg catgatgtgt aaagacttga cttgttagag attaaagaat
     4081 tgaccatgaa catccacgaa gccaccgccc aagatgccgc taccctcgcc ctgctgatca
     4141 gcgcgtccaa ccaggacgtg gccgcccggt ttggcctgac cctggacaac tgcccgaaac
     4201 acccgtcgtt ctgcaacgcc gactggatca gagctgatct ggcgcgcggc gagcgctatt
     4261 ttctggccga acaagcatcc ggccccatgg cctgcgtggc gtttgagctg gccggcgcgg
     4321 gtgtgggtta cctgaaccgc ttgtcggtgc tgcctgcgca ccggcgctgc ggtgtcggcg
     4381 cgcggttggt ccagcatgtg ttggcgctgg cccaatccag cgggcttgag cgcgtcagca
     4441 tcggcgtgat tgggcagcac atggcgttgc agcgctggta cagcaagctc ggttttgtca
     4501 cgggtgacac ccagcgtttt gcacatctgc cgttttcagt gacatacatg agctgcgtcg
     4561 tgcgggggtg aacgcagtct tcgggcctgc tgcccggcgc tgcggccatc ctgggcagcg
     4621 caccgactct tgcctcgatg aattcacaca cagcctgtgc gtttggcgaa cgccgttatc
     4681 aagcctggag cgaacaggtc aacacccgct acggcggatg cgtgcaaaag gtgtttgtcg
     4741 ccaaccttcc caaaggcgcg caaatcagga aattgttcgt ccctatcggc gtggattgca
     4801 gacccccttg tggcacccga ctgtgatgcc gctcagcccg gcaacgggtg acaggccaag
     4861 gtggtgattc ggactctctg ctgggcagcg ctgcgtctgg catg
//
LOCUS       DAOSLL010000349         4898 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15396_length_4898_cov_4.036548, whole genome shotgun sequence.
ACCESSION   DAOSLL010000349 DAOSLL010000000
VERSION     DAOSLL010000349.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4898)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4898
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15396_length_4898_cov_4.036548"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..375)
                     /locus_tag="PLB25_14395"
     CDS             complement(7..375)
                     /locus_tag="PLB25_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42801.1"
                     /translation="MNKIAPCLVSPALKRTGHYGDVLPDDVGLAQIRLDWHVCPRVPQ
                     DVLYEAMNRDDWTWRSLPAGIAQTGARALSSTACDTAIFLDDSIKECHGKKMPGISGH
                     FDHTRGRNVMGWQSTFTSIV"
     gene            387..851
                     /locus_tag="PLB25_14400"
     CDS             387..851
                     /locus_tag="PLB25_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42802.1"
                     /translation="MKPNALSACMATLQVDQPRGFVATPFDANVIFDGDGYPHLEFGS
                     NAASLQFMAPQAGQAAGNPADLTCNFNVDAPYQVLVARSSPCRLTTIAGVGAVLLSLT
                     RMGSCPASSATGSQTPGSSPMSSHRPMPKRVAQLSDLILLGGDARPSKQVQI"
     gene            848..1348
                     /locus_tag="PLB25_14405"
     CDS             848..1348
                     /locus_tag="PLB25_14405"
                     /inference="COORDINATES: protein motif:HMM:NF039682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6194 family protein"
                     /protein_id="HOE42803.1"
                     /translation="MTPGDIANDICSRFAGVNPKASWGETALFYNPGHQLPHGVYFCT
                     IKQHDGENDKASRLDRDGVFRVAIGLAPATYARLFGKKPERPGKGECVATGHDFTALN
                     VLMPHPVYAWMGWAQILNPSQERLTEIGTLIDEAYRAAVAKFEKKTARKSVVALRAAS
                     LAAQLR"
     gene            1484..2104
                     /locus_tag="PLB25_14410"
     CDS             1484..2104
                     /locus_tag="PLB25_14410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011616258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HOE42804.1"
                     /translation="MFEIQNYASFVAAIFIFQLIPGAGTLAILNATARNGIGAGLGAV
                     LGTLVGDFIFMVAAVAGLAAFMNANPYLFRGLQWFGAGYLCWLGLQLLRAPVDKGESA
                     AEPGKTGWVYFRQACLVSLTNPKVMLFFVAFFPLFMRPGASGATLVAMMAHVTLISLV
                     YQACIVLVGNAVARRLASIASARRLATRVAGIALLGFGIKLAAANQ"
     gene            complement(2361..3356)
                     /locus_tag="PLB25_14415"
     CDS             complement(2361..3356)
                     /locus_tag="PLB25_14415"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012352830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HOE42805.1"
                     /translation="MKIAVFSAKRYDRDFLNAANAGAGHQLRYHDDPLDLETVALAAG
                     CEAVCVFVNDTVDAEVLNKLAAGGTRLVALRCTGFNNVDLKAAQQLGLKVVRVVTYSP
                     YSVAEHAVALLQAINRKIHRAYNRTRDSNFALDGLMGFDLHGKTVAVVGTGKIGRVFA
                     QIMLGFGCKVIGYDKFPSPEFEALGARYAKVGEIGETADIISLHCPLTPETHHIVNAG
                     MLARAKHGALLINTSRGGLIDTEAAIEALKSGQLGGLALDVYEQEADLFFRDLSNTII
                     SDDVFQRLLSFPNVIVTGHQAFFTQEAIATICETTIQSVTEFASGQPLSNEIKCT"
     gene            3702..4358
                     /locus_tag="PLB25_14420"
     CDS             3702..4358
                     /locus_tag="PLB25_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42806.1"
                     /translation="MLKPRFLTTARQLGLAAALAFGANAWAVPEAQFAPAFEAFSQAR
                     SGNGTAIDKSVASFTALLAAEPGNPVLLAYLGASTAMQSNSTWLPWKKMNYAEDGLAM
                     LDKALALLTPAHNAALQHAVPAVLEVRFTAANTFLAVPGFMNRSARGASLLAEVTASP
                     LLAAAPMAFRGDVWLVAAEQAIKDKRIVEARRFLNDIVKNSAPQAATARARLNDLPQS
                     "
     gene            4355..>4898
                     /locus_tag="PLB25_14425"
     CDS             4355..>4898
                     /locus_tag="PLB25_14425"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE42807.1"
                     /translation="MSTAKTQAVLAMHEVHKTYRLGAHVIPALQGVDLSVQRGELLAL
                     TGPSGSGKSTILNLAGLIDTPDSGDILLDGQPVNGLGEVQRTLLRRDAMGFVFQSFNL
                     VPVMTVFENVEYPLFIAGVPPAERRDRVTAQLQAVGLQDHARHRPDALSGGQRQRVAI
                     ARALVKRPKLVIADEPTANLD"
BASE COUNT          957 a         1478 c         1473 g          990 t
ORIGIN      
        1 agccagctac acgattgagg tgaacgttga ctgccaaccc atgacattgc ggcccctcgt
       61 gtggtcaaaa tgacccgata tgccaggcat ctttttgcca tggcactcct tgatggagtc
      121 atcgagaaaa atagccgtgt cgcaggcggt cgaactcaac gctcgtgctc ctgtttgagc
      181 aatgccagca ggcaaggagc gccaagtcca gtcatcgcgg ttcatggctt catacagaac
      241 gtcttgaggg actcgcgggc aaacatgcca atcgagtcgg atttgagcca gacccacatc
      301 atcaggcagt acatcaccat aatgaccggt acgcttaagc gctggcgaaa ccaggcaggg
      361 tgcgatcttg ttcaaggaga ttcgaattga aacccaatgc cctatcggcc tgcatggcaa
      421 ccttacaggt tgaccagccg cgtggatttg ttgccacgcc tttcgacgcc aacgtcattt
      481 ttgacggcga cgggtatccg caccttgagt ttggcagcaa cgccgcgtca ttgcagttca
      541 tggcaccgca agctgggcag gcggctggca acccagccga cttgacctgc aacttcaatg
      601 tcgatgcgcc ctaccaggtg ctggtggcgc ggtcatcgcc atgccgcctg acgaccattg
      661 ccggggtggg cgcggttttg ctgtcactga cccgaatggg gtcatgccct gcatcttctg
      721 cgaccgggag ccaaaccccg ggttcaagcc ctatgtcaag ccatagacca atgccaaagc
      781 gagtcgcaca gttgtcggat ctgatcctgt tgggtggcga tgcgcggccg tccaagcagg
      841 tgcagatatg acacccggcg acattgccaa tgacatctgc agcaggttcg ccggggtcaa
      901 ccccaaggcg agctggggtg aaaccgcgtt gttttacaac cctggccatc aactgcccca
      961 cggcgtttac ttttgcacca tcaagcagca tgacggcgaa aacgacaaag cctccaggct
     1021 tgatcgtgat ggcgtattca gagtcgccat tggtcttgcg cccgccacct atgccaggct
     1081 gttcggaaaa aagcccgagc gaccaggcaa gggtgaatgc gtggcaaccg gccatgattt
     1141 cacggccttg aatgtgctga tgccgcatcc ggtttacgcc tggatgggct gggcccagat
     1201 cctcaaccca agtcaggaac gcttgactga aatcggcacg ctgatcgatg aggcctatcg
     1261 ggccgcggtg gcaaagttcg aaaagaaaac cgcccgcaaa tcagtcgttg ctttgcgcgc
     1321 cgcgtcgctc gctgcccagt tgcgttgacc ggtgcgccgt ggcagcccgg cttgccgtgc
     1381 cagtacccgc cacgcaattg cagaccggtt tttttgctgg tcagtctgtc ggttatcatt
     1441 tttgcaaccc agcgtttgca agccgacatg aaggaaatga cctgtgtttg agatccagaa
     1501 ctacgcaagc tttgttgccg caatcttcat ttttcagttg atacccggag ccggtacgct
     1561 ggccatcctc aacgccacgg ctcgcaacgg tattggcgct ggtctcggcg ccgtgctggg
     1621 aaccttggtc ggcgacttta tcttcatggt tgccgcagtg gccggtctgg cagccttcat
     1681 gaatgccaat ccgtatcttt tcaggggcct tcaatggttt ggtgccgggt acctgtgctg
     1741 gctgggtctg caactgcttc gggcaccggt tgacaagggt gagtcggctg ctgaaccagg
     1801 caagacgggc tgggtctatt ttcgccaggc gtgtctggtc agcctgacca acccgaaggt
     1861 gatgctcttc tttgtggcat ttttcccgtt gttcatgcgt cctggggcat ctggtgccac
     1921 gctggttgcg atgatggctc acgtgacgct catcagcctg gtttaccagg cctgcatcgt
     1981 gctggttggc aatgccgtgg cgcgccgcct tgcgtccatt gcatcggctc gcaggctggc
     2041 aacccgtgtt gcgggcatcg ccttgcttgg ttttggcatc aagctggcag cggctaacca
     2101 gtgaacgatg gccgaacatg ttgacgcggg caccgcctgc agcctgaccc tggtcaatgc
     2161 caccgagccc tctgatgcgc cgggcgcgca tgaaacaacg cgctggcgat caatgaccag
     2221 cctctcggtc tgcagtcggc aagccgaccg acggacgcca gatcgtcttg tgctgtgttg
     2281 gcctgtcggt cgcttcaact gcccgtgcgc aggctgatgt tcaacgcttc ggacctgccg
     2341 gtcattgcga ggtggcgaaa tcaggtgcac ttgatctcgt tggacagtgg ctgaccgctg
     2401 gcgaactcgg tgacgctctg gatggtagtt tcacagatcg tggcgatggc ttcctgggta
     2461 aaaaacgcct ggtgcccggt cactatcaca ttcgggaaag aaagcaggcg ctggaatacg
     2521 tcgtcggaaa tgatggtgtt cgacaaatcg cgaaagaaca gatcggcttc ctgctcgtaa
     2581 acatcaaggg ccagcccgcc cagttgtccg ctcttgagcg cttcgatggc ggcctcggtg
     2641 tcgatcaggc cgccgcggct ggtattgatc agcaaagcac cgtgtttggc gcgcgccaac
     2701 atgcctgcat tgacgatgtg gtgggtttcg ggtgtcagcg gacagtgcag ggagatgata
     2761 tcggccgttt caccaatctc gccaaccttg gcatagcggg caccaagcgc ttcaaattca
     2821 ggcgatggaa acttgtcata gccaatcacc ttgcatccaa agccgagcat gatctgggca
     2881 aagacccgcc cgatcttgcc ggttccgacc accgccacgg tcttgccgtg caggtcgaag
     2941 cccatcaaac catcaagggc gaaattggag tcacgcgtgc ggttataggc ccggtgaatc
     3001 ttgcggttga tggcctgcaa cagcgccacc gcatgttcag ccaccgaata gggcgaatag
     3061 gtcaccaccc gcaccacctt gaggcccaac tgttgcgccg ctttcaaatc cacattgttg
     3121 aagccggtac agcgcaaggc gacgaggcgt gtgccgccag cggcgagttt gttgagcacc
     3181 tcagcatcga cggtgtcgtt gacaaaaacg cagaccgcct cgcagccagc ggccagcgcc
     3241 acggtttcca gatccagcgg gtcgtcgtga tagcgaagct ggtgcccggc accagcgttg
     3301 gcagcgttca ggaagtcacg gtcgtagcgc ttggcgctga atacggcaat tttcatcgga
     3361 gaggctccaa aagtttgtgt cgaacatgac gatgacggcc tgaccaacat ccggttggtc
     3421 ggacgatgcg cggtgcctgg acgacgcgag tatcaccgga ttgccgacgg cagtgcaaag
     3481 tggcagggta atgccaccgg tgctcacatt ggcacggaaa tactggctca atgcaagtgc
     3541 gctggccagg aaaacaagga ccctatggct gcattcacgc cgccgagtcg gcgtttcgcc
     3601 cgcgccagcg gcatttggtc gcaacagggc tgcacgcaga agcgggcttg cctaaagttc
     3661 aacccatggc aacttttttc aacctttttc aggagtccaa catgctcaaa ccccgcttcc
     3721 taaccaccgc ccgccaattg gggctggctg ccgcactggc ctttggtgcc aacgcctggg
     3781 cagtgcccga ggcccaattt gcgccagcct ttgaggcgtt ctcccaggcc cgctccggca
     3841 atggcaccgc catcgacaaa tcggtcgcct cctttaccgc gctgctggcc gcagagccgg
     3901 gcaacccggt gctgctggcc tacctgggtg ccagcaccgc gatgcagtcc aatagcacct
     3961 ggctgccgtg gaagaagatg aactacgccg aagacggcct ggccatgctg gacaaggcgc
     4021 tggcactgct gaccccggcg cacaacgctg ccttgcaaca cgcggtgcca gcggtgctgg
     4081 aggtgcgctt taccgccgcc aacacctttc tggcggtgcc cggcttcatg aaccgcagcg
     4141 cccgcggtgc cagtttgctg gccgaggtga ctgccagccc cttgctggcg gcagcaccga
     4201 tggcctttcg tggcgatgtc tggctggtgg ctgcagagca ggccatcaag gacaagcgca
     4261 ttgttgaggc gcgccggttt ctgaacgaca tcgtcaagaa cagcgcgccg caagcggcaa
     4321 ctgcacgcgc ccgcctcaac gatctgcctc aatcatgagc acggccaaga cgcaagccgt
     4381 gctggccatg cacgaggtgc acaaaaccta ccggctcggt gcccatgtga tcccggcctt
     4441 gcaaggggtg gacttgagcg tgcagcgcgg cgaactgctg gcgctgaccg gcccctcggg
     4501 cagcggcaaa agcaccatcc tgaacctggc cgggctgatt gacacgccag attcgggcga
     4561 catcctgctc gatggccagc ctgtcaacgg tctgggcgaa gtgcaacgca ccctgctgcg
     4621 ccgcgatgcc atgggctttg tgttccagag ctttaacctg gtgccggtga tgacggtgtt
     4681 tgaaaacgtc gaatacccgc tgttcattgc cggtgtgccg cccgcagagc ggcgcgaccg
     4741 ggtaacggcg caactgcagg cggtgggctt gcaggaccat gcccgccatc gccccgatgc
     4801 gctgtccggt ggccagcgcc agcgggtggc gattgcgcga gcgctggtga agcgccccaa
     4861 gctggtgatt gccgacgaac ccaccgccaa cctggact
//
LOCUS       DAOSLL010000350         4893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15418_length_4893_cov_5.511782, whole genome shotgun sequence.
ACCESSION   DAOSLL010000350 DAOSLL010000000
VERSION     DAOSLL010000350.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15418_length_4893_cov_5.511782"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(31..712)
                     /locus_tag="PLB25_14430"
                     /pseudo
     CDS             complement(31..712)
                     /locus_tag="PLB25_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879703.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            1078..3114
                     /locus_tag="PLB25_14435"
     CDS             1078..3114
                     /locus_tag="PLB25_14435"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOE42808.1"
                     /translation="MAHGGYDSKFENPELRSHAMRLRMKVYLGVFLVSLVLGLVVNFS
                     RSPIFLASARVQITPPGNVATAAPPAAAIDKGAQQAILIEMEVLNSSPLLEKTGEQLR
                     AQGLLSNGSSETVSTLQAMLKLGNVEGTPIVTLQAQGSQKSLLAPLLNTLLEIYRLHQ
                     TEAGDASWQAQLHAAKDEIDVVEAKFQEKQNALEALRSRADISSAERNENQTLIHLKG
                     LTTSLTDATNREATAAGHVRAIEQAITQGRRSYSAKDNQTVAAIEARLSQAKEDWRAL
                     ERQFTSKYLDMDANAIALKVRISNLEQQLESERQKSQQAALAGALEELTSAQATAQRL
                     QQQIISGKQEVNTVNRFLGQMQSLQDELKSLALVRNSAAQKLLALQSSETARKPRLVI
                     LEPAVAPDAPSQPQYWRDAGIVLIASAVLGFLSVWFVEFFNRKEAAPAGPATVVLPPA
                     WMVHPQAMAALNASSNTAARLPLHGELAQQLLTNPLPRELDASEVQQLLQAATPKNLP
                     LLTCLLCGLGAEEVVSLQWQHLDTRNNVLQVPGESSRELPLSSQLRIWAEISFANRDT
                     MPPSTPIFTQGAGKPLEVADVQAIVASSAVDSCLVAPQSITPAALRHTYVAFLVRQGL
                     RFSELGVLVGRLNADMLNALAPLVQAAPTGSRVGVAEVQQLLPALRAKLASENV"
     gene            3892..4791
                     /locus_tag="PLB25_14440"
     CDS             3892..4791
                     /locus_tag="PLB25_14440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE42809.1"
                     /translation="MTLDKTTMIANLQDTLISTIKRANQSPACLIVVHGPLQGKRFEI
                     SAKSMRLGRDTSADIVIDDPKVSRKQATVQSEAGVVSLIDGGSTNGTAINDARLHRGE
                     MAVLRRDDLIKVGGTVLKFLPQGELDSRFIDALETRAHVDALTQTYNKGYILEVLEAE
                     FRKAQVLSTPLSLLMVDLDHFKTINDQRGHDAGDQVLIDVSQLLKQAVAGSHAVLGRF
                     GGEEFLLLLPGLDKPASVRLAEDIRHRVELQPVIYDSHRIHLTISLGVATKTAESDSA
                     RELFKQADRALYQAKSAGRNRVC"
BASE COUNT         1146 a         1374 c         1284 g         1089 t
ORIGIN      
        1 ttttttcagc gagaacactc cccggcgcac ttatgctgcc agtgcataaa actgttcggc
       61 gaacgagaga tcctttcctt tttcggcgct caactgacct ttgcggatca agtgcatcaa
      121 ctcaattccg gccagcacag ctttggctgc caagaatgat ttgaatccca gcatcggctt
      181 tgtgatgcgt ttgaccgcac gatggtcttg ctcagcgatg ttgttgagat acttgatctg
      241 gcgcactgca atgggtactt tttgctcttt gttgatcgcg tccatggccg ctttgttcgc
      301 cccactttta tccatcgtga ccttctctgg ctcaccgttt ggccgcatcg ccgtcttgaa
      361 atacgccatc gccgcagctt tgtctcgttt ggcgcgtagc agaaaatcaa cggtcgcaca
      421 ctctttatct accgcacgat acaggtactt ccattcgcca ccaaccttga tatacgtctc
      481 atccatgcgc caactgcgcc ccaccgggcg cttatgctct ctgaatgaat actttctcga
      541 gcatcggtaa aaatttaatc gcccagcgat tgagggtcga gtggtcaaca aacacaccgc
      601 gctcctgcat catcttttca aggttacgat agctcagcgc gtatgccgcg taccagcgta
      661 tacacaccag aatgctttcc aatggaaatc tcatcgcctt catattcagc atcgcccaat
      721 ctccgtcacg caccagcccg caaggatacc ggctaatgcg agagaaccca aagccaatga
      781 aatggcggcg ctacatccca aaaagcgtgg ccccaacccg atctgctacg cgccgaaatg
      841 gtcgctcttg agaaccacca tgcgtatggt ggtgcgcttt tgctgtcgag ttttgtccgg
      901 gctacgcgaa acccaaatca atagtgattt cgaaaccctg caagtgcttc tcatccaact
      961 gataagataa atcactggca tttcaaatgc tggagccaga aagtcagtta cttggtttga
     1021 gacccgatgc aaaatacgcc ccattcgccg cagtacgact acgctgacga gctgcgcatg
     1081 gcccatggcg gctacgattc aaaatttgaa aaccccgaac ttcgtagcca tgccatgcgg
     1141 ctccgaatga aagtgtattt aggcgtgttc ctcgtcagct tggtgctggg tttggtggtc
     1201 aatttctcgc gaagtcctat tttcctggcc tcggcgcgtg tgcaaatcac cccgcccgga
     1261 aatgttgcca ctgcagcacc accggctgcg gccattgaca aaggcgcgca gcaagccata
     1321 ttgatcgaaa tggaagttct caatagcagc ccattgctgg agaaaactgg cgaacaattg
     1381 cgagctcagg gactgctcag caatggttcc agcgaaaccg tgtcgaccct gcaagcgatg
     1441 ctgaagttgg gcaatgtgga gggaacccct atagtaacgc tgcaggcaca aggcagtcag
     1501 aagtccttgt tggcacctct tctcaatacc ttgttggaaa tatatcgctt acatcagacc
     1561 gaggcgggag acgccagttg gcaagcacag ttgcacgctg cgaaagatga gatcgatgtc
     1621 gttgaagcca agtttcagga aaagcaaaac gcgttggagg cgctgcgttc gcgtgccgat
     1681 atcagctccg cagagcgcaa cgaaaaccag accctgatcc acctcaaggg cctgacaacc
     1741 tcattgactg atgctaccaa tcgtgaagcc actgctgccg gtcatgttcg cgccatcgag
     1801 caagccatta cgcaaggcag gcgcagctat tcggcgaaag acaaccaaac agtcgcggct
     1861 attgaagcgc gtttgtcgca agcaaaagag gactggcgag ccttggagcg ccaattcaca
     1921 tcaaaatacc tcgacatgga cgcaaatgct atcgccctaa aggtccgaat ctccaatctg
     1981 gaacaacaac tggagtccga gcgtcaaaag tcccaacaag ccgcgctcgc tggtgcgctc
     2041 gaagagctca ccagcgcgca ggctactgca cagcgcctgc aacaacagat tatttccggt
     2101 aagcaggaag tgaacacggt caatcgtttt ttggggcaaa tgcaaagcct gcaggacgaa
     2161 ctaaagagcc ttgcgctggt gcgtaacagc gcggcgcaga agctgctggc acttcaatcc
     2221 agtgaaactg cccgcaagcc tcgtttggtt attctggagc ctgccgtggc accagatgcg
     2281 ccttcacagc ctcagtattg gcgggatgcg ggcattgtgc tgattgcttc agccgttctt
     2341 ggctttttgt cggtgtggtt tgttgaattt tttaaccgca aagaggctgc accggctggg
     2401 cccgccacgg tggtactacc cccagcatgg atggttcatc cccaagcgat ggctgccctc
     2461 aacgccagca gcaatactgc agctcggttg cccttgcacg gtgaactagc acaacagttg
     2521 ctgaccaatc cactgcctcg ggaacttgat gccagtgaag ttcaacaact tctgcaagca
     2581 gccacaccaa agaacttgcc actactgacc tgtcttcttt gcgggctggg tgcggaggaa
     2641 gtggtaagtc tgcaatggca gcacctcgac acaagaaata atgtgcttca ggtacctggc
     2701 gagtcgagcc gggaattgcc actgagttcg caactgcgta tctgggccga aatttcattt
     2761 gccaaccgtg acaccatgcc accctcaact ccaattttta ctcaaggcgc aggcaagcca
     2821 ttggaagtcg cagatgtgca ggccatcgtt gcttcaagcg cggttgacag ttgtcttgtc
     2881 gcgccacaaa gcattacacc agcggcttta cgtcacacct atgttgcttt tttggtgcgc
     2941 caaggcttgc gttttagtga gcttggagta ttggttggtc gtctgaacgc ggacatgctc
     3001 aacgcactgg ctcctttggt gcaagctgca ccgacgggtt cgcgggttgg ggtagctgag
     3061 gtgcagcagt tgttgcctgc gctacgcgcc aaattggcgt cggagaatgt ttgaatatgt
     3121 atggcaggcg tggcttttta gacaaatcgg gattttttgt gaaacttgcg ccaatcgcgc
     3181 agagttcgtt gagagatgaa gggcagtgca ggattgggaa actcgaaatt gctcgatggc
     3241 aacagaagac tcctcctttt gcccattctc gacggctcgg catttcgaaa ctgccgcacc
     3301 tttttgattt ccaggcagac agcctgttga tttcaacggc tgctatcgat gagggaggca
     3361 tttcggaaaa ctgcctgacc gcaacgggtc ttgcggagcc agccaacttt atcgggggtc
     3421 gatattcaaa attgagcatc agcaatgggg gaagacgcat atcgttacac acaagtctcc
     3481 aactccagac tgtcccgtga attcaccgaa gattcgcaaa atcccgaact tattgtggga
     3541 gggatgctca cgctagtgtt accggaaaac cgccgctttt gaaccacgcc ccgagcctta
     3601 cgccgctgcc caatttgcgc gacgattggc gacttgccca caccgggtcg gaccctcagc
     3661 atgacggggg tgcggtgacg ctcggccgca ctgataaatt cccggccaag aacaaagtta
     3721 agctgggcgg gacgcagcgg gcacaggttg cctcaacaag gctatagttc aaacaagtgc
     3781 cagactttac ccgggtttag acaaacacgg ccgtgggcgt ttgttaccat aggcatgtca
     3841 gcccaccgtg cagcaatcca aagcgcggac attgccattt aattgcgccc aatgaccctt
     3901 gacaaaacca cgatgattgc caatctgcaa gacaccttga tcagcacgat caagcgtgcc
     3961 aatcagagcc ccgcctgcct gatcgtggtg catggaccgc tgcagggcaa gcgctttgaa
     4021 atcagtgcca aaagcatgcg cctgggtcgc gacaccagtg ctgacatcgt gattgacgac
     4081 cccaaggtct cgcgcaagca ggccacggtg cagagcgagg ccggagtggt gagcctgatc
     4141 gatggtggat caaccaatgg caccgcgatc aacgacgctc gtttgcaccg cggcgagatg
     4201 gctgttctgc gacgcgacga cctcatcaag gtgggaggca cggtgctgaa gtttttgccg
     4261 caaggcgaac ttgattctcg ctttatcgac gcacttgaga cccgtgccca cgttgatgcc
     4321 ctcacccaga cctataacaa gggctacatc ttggaggtgc ttgaagccga gtttcgcaag
     4381 gcgcaggtgc tgagcacgcc cttgagcctg ttgatggtcg acctggacca tttcaaaacc
     4441 atcaatgacc agcgcggcca tgatgccggc gatcaggtgc tgatcgatgt cagccaactg
     4501 ctcaagcaag ccgtggcggg cagccacgcg gtgctggggc gatttggcgg cgaagagttt
     4561 ctgctactgc tgccggggct ggacaagccc gcctcggttc gtctcgcaga agacatccgc
     4621 caccgggttg agctccaacc agtgatttac gatagccacc gcatccatct gaccatcagt
     4681 ttgggcgttg ccaccaagac cgccgagtca gacagcgcac gcgaactctt caaacaggcc
     4741 gaccgcgccc tgtaccaggc caagagcgcg gggcgaaacc gggtctgcta attatcaact
     4801 ccagcttaag ttcattcgtg ttaacctaaa gttcttttcc aaaaaagccg agtttcccca
     4861 atgaaaccca cgttaatttt ggttccgcgt tct
//
LOCUS       DAOSLL010000351         4872 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15495_length_4872_cov_3.836413, whole genome shotgun sequence.
ACCESSION   DAOSLL010000351 DAOSLL010000000
VERSION     DAOSLL010000351.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15495_length_4872_cov_3.836413"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..1311)
                     /locus_tag="PLB25_14445"
     CDS             complement(154..1311)
                     /locus_tag="PLB25_14445"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HOE42810.1"
                     /translation="MIARAPVPVDLTSLSRSRPSQVPLTCALLAINLLLFVLMQADGA
                     GFWHSSSSVPLAWGASFGPATQDGQWWRLVTAQFVHFGLLHLGLNMWALWDVGRLVER
                     PYGPLRFGLLYLGSGLVGNLLSLAVQGNQAVSGGASGAIFGLYGALLVFLWRERGQVD
                     RRDFRWAFGVAVIFTLTSLGLGWLIPGVDNAAHLGGLVAGACLGTLLARRWLPDSPAP
                     LRERWLAAVALASGIAALLTHLPAPLYLMSEERRAREAIQQFLVIDQQLSQRWNQLMT
                     VRLGGPGHARVSFDQLASRIQTSITSVYDNSFKQLAAARPTTAAPSAKTLEALQNYAG
                     LRAEASRELADGLRRQDQGKILAALEKARKAHASVQAPAASGPAAPDHNLR"
     gene            complement(1308..1910)
                     /locus_tag="PLB25_14450"
     CDS             complement(1308..1910)
                     /locus_tag="PLB25_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HOE42811.1"
                     /translation="MAERARQRLLQASVQVKAARQAATDRELFVKAAGAVQPLPDKRS
                     VLHKKEPKMPLAMQYQKDEKAVLREAISDEFDVSTLLEADEHLSFRRPGIGPDVTRKL
                     RRGDWSIQRQIDLHGLRRDDAREVLSIFIREAHKHGIRCVRVVHGKGLGSPGKAPVLK
                     SRVHSWLVQKSEVLAFVQAKPADGGAGALVVLLMTARGSG"
     gene            complement(1976..2677)
                     /gene="radC"
                     /locus_tag="PLB25_14455"
     CDS             complement(1976..2677)
                     /gene="radC"
                     /locus_tag="PLB25_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HOE42812.1"
                     /translation="MPLKDLPPDARPREKMLALGPASLSDVELLALLLRTGIKGKGVL
                     QMADELLKLKPAAGTDAGFDGIAGLLNATTDDLRRVKGLGPAKRSELAAVLELARRAM
                     RQRLQERTVFNSPDAVKHYLQLHLSSRTHEVFAVLFLDVQNRLLSFEELFHGTLTQTS
                     VYPREVVLRALHHQASAVVLSHNHPSGTVQPSRADEMLTQTLKTTLALIDVRVLDHVI
                     VAPGEALSMAERGLL"
     gene            2802..3266
                     /locus_tag="PLB25_14460"
     CDS             2802..3266
                     /locus_tag="PLB25_14460"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOE42813.1"
                     /translation="MTSTSTTVQPGSFLTLHYRLSGPAGNVINTFDGKPATLTMGTGQ
                     LSPTLEAYLLDLPEGSRSTFELPAGTAFGPRNPDMLQWMARQELLDMGAAGEQYAVGD
                     VLEFPTPDGTGKFAGVIVDFRTDAKGDAVLFDFNHPLAGQPVTFEVHVIGVL"
     gene            3263..4240
                     /gene="ispH"
                     /locus_tag="PLB25_14465"
                     /gene_synonym="lytB"
     CDS             3263..4240
                     /gene="ispH"
                     /locus_tag="PLB25_14465"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOE42814.1"
                     /translation="MTGLQSGAASILLAEPRGFCAGVDRAIEIVEKALAKFGAPIYVR
                     HEIVHNTYVVNDLKAKGAIFIEDLADVPPGATLVFSAHGVSRAVRVEADDRGFQVFDA
                     TCPLVTKVHVEVAKLAKEGYEFIMIGHKGHPEVEGTMGQLSDGIYLVEDVADVARVQP
                     RGRQLAVVTQTTLSVDDAAAILAEVKRLFPHVREPKKQDICYATQNRQDAVKVMAPQV
                     DIVIVVGSPTSSNSNRLRELAERLGTPAHMVDSADDLQPAWFAGHPRVGLTAGASAPD
                     VLVQQVINRLRALGAISVRRMAGVEETVHFPLPKGLGDKSMSEFAASRS"
     gene            4326..>4872
                     /locus_tag="PLB25_14470"
     CDS             4326..>4872
                     /locus_tag="PLB25_14470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOE42815.1"
                     /translation="MLDINLLRRDLPAVIAALEARKSPQPFLDVARFTALETDRKTLQ
                     VRTEELQARRNALSKQIGQAKGKGEDASALMAEVGGIAEEMKAGGERLEAIQAELATM
                     LMSVPNLPDASVPVGADEQANQEVRRWGTPGSYGFAVKDHVDLGAPLGLDFETGAKLS
                     GARFSFLKGPAARLHRALAQFM"
BASE COUNT          895 a         1599 c         1544 g          834 t
ORIGIN      
        1 tttgaatgtt tgaattcttc gcatgatatg caaagacttg acttgttaga gaccagattc
       61 ctcagttgga cgcgcccgag tgggccgctg gcggcgcgtc tgggttggcg cgacgacgca
      121 gtgggctgct gcgcctttga tgccaaatcc ggtttaccga aggttgtggt cgggcgctgc
      181 cgggccgctt gctgcagggg cctggacgct tgcgtgcgcc ttgcgggctt tttccagtgc
      241 cgccagaatt ttgccctgat cctgccggcg caggccatcg gccagttcgc gcgaggcctc
      301 ggcgcgcagc cccgcgtaat tttgcaaagc ctccagtgtc ttggcagatg gcgcagcagt
      361 ggtcggcctg gcggctgcca gttgcttgaa actgttgtcg taaacgctgg tgatgctggt
      421 ctgaatacgg ctggccagtt gatcaaatga aacacgagcg tgacccggcc cgcccaggcg
      481 cacggtcata agctggttcc agcgctggct gagctgctgg tcaatgacca aaaactgttg
      541 aattgcctcg cgcgccctgc gctcctcact catcaggtag agcggtgctg gcagatgggt
      601 caacagcgct gcgatgccgc tggccagcgc cacggctgcc agccagcgct cacgcagcgg
      661 ggccgggctg tcgggcagcc agcgccgcgc cagcagggtg cccaggcagg caccagcgac
      721 cagtccaccc agatgcgctg cattgtcaac gccgggtatc agccagccca ggcccagcga
      781 agtcagggta aaaatgaccg ctacgccaaa cgcccaacgg aagtcgcgcc ggtcaacttg
      841 tccgcgttcg cgccataaaa acaccagcag cgcgccgtac aggccaaaaa tggcccccga
      901 agcaccacca gataccgcct ggttgccctg caccgccagc gacaacaggt tgccgaccag
      961 gccactgccc agatacagca ggccaaaccg cagcggtcca tagggccgct caaccagtcg
     1021 gccgacatcc cacagcgccc acatgttcag gcccaggtgc agcagcccaa aatgaacaaa
     1081 ctgggcagtt acaagccgcc accactggcc gtcctgggtg gctggcccaa agctggcacc
     1141 ccaggccagc ggcacgctgc tgctgctgtg ccagaagcca gcgccgtctg cctgcatcag
     1201 cacaaagagc agcaggttga tcgccagcag cgcgcaagtc aggggaacct ggctcgggcg
     1261 actgcgcgac aagctggtca ggtcaaccgg cacgggtgca cgcgctatca cccagagcct
     1321 ctggcggtca tcagcaacac caccagcgca ccggcaccgc catcggccgg tttggcctgc
     1381 acgaaggcca gcacctcgct tttttgcacc agccaactgt ggacccggct tttcagcacc
     1441 ggggccttgc cgggcgagcc cagtcccttg ccatgcacca cccgcacgca gcggatgccg
     1501 tgcttgtgtg cttcacggat gaaaatgctc aagacctcgc gggcatcgtc acggcgcagg
     1561 ccgtgcaggt caatctggcg ctgaatgctc cagtcgccac ggcgcagctt gcgggttaca
     1621 tcgggcccga tgccgggtcg ccgaaaactc aaatgctcgt cagcttcgag cagggtgctg
     1681 acatcaaatt cgtcgctgat ggcctcgcgc agcaccgcct tttcgtcttt ttggtactgc
     1741 attgccaatg gcatttttgg ctccttttta tgcagcacgc tgcgcttgtc tggcaagggc
     1801 tggacggctc cagcggcctt gacgaacaat tcacggtcgg tggccgcctg gcgggctgcc
     1861 ttgacctgca cgctggcttg cagcaggcgc tgccgggcgc gctcggccat ggcccgcttg
     1921 acttgcttga gttcggtcag ggcggtcacc ttgacgacac tggcgcgctg cccgatcaca
     1981 gcaaaccccg ctccgccatg ctgagcgcct caccaggggc gacgatgacg tggtcaagca
     2041 cccgcacgtc gatcagtgcc agcgtggttt tcagggtttg cgtcaacatc tcgtcggccc
     2101 ttgacggttg caccgtgccg ctcgggtggt tgtgcgacag caccaccgca ctggcctggt
     2161 ggtgcagcgc ccgcagcacc acctcacgcg gatagacact ggtttgcgtc agcgtgccgt
     2221 gaaacaattc ctcgaatgac agcaagcggt tttgcacgtc cagaaacaac accgcaaaca
     2281 cttcgtgcgt gcgcgaagac aggtgcagtt gcaggtagtg cttgacggca tcgggcgagt
     2341 taaacacggt gcgctcctgc agccgctggc gcatggcacg gcgcgccagt tccagcaccg
     2401 ccgccagttc agagcgtttg gccggcccca ggcccttgac gcgcctgagg tcatcggtgg
     2461 tggcgttgag cagcccggca atgccgtcaa aacccgcgtc ggttccggca gcgggcttga
     2521 gcttgagcag ttcatcggcc atttgcagca cccccttgcc cttgatgccg gtgcgcagca
     2581 gcagcgccag cagttccacg tcactcaggg aagcggggcc aagcgccagc attttttcac
     2641 gcgggcgggc atcggggggc aggtctttga gtggcatcgg tagcggcaag aaggcgggct
     2701 tttacaatgc gtgcagttta tgcgccagca gaccccaatt tctgacatcg gggctttgtt
     2761 gcgacgcagc atctcttttt accttcaggc cacgcatcac catgacaagc accagcacca
     2821 ccgtccaacc cggttctttc ctgaccctgc attaccgcct gagcggcccg gcaggcaatg
     2881 tgatcaacac atttgatggc aaacccgcca cgctgaccat gggcaccggg caactatcac
     2941 cgacgctgga agcctatctg ctggacttgc ccgaaggcag ccgcagcacc ttcgagctgc
     3001 ccgctggcac cgcttttggc ccgcgcaacc ccgacatgct gcaatggatg gcgcgccagg
     3061 agctgcttga catgggcgcg gcgggcgagc aatacgcggt gggcgacgtg cttgaatttc
     3121 ccacccccga cggcaccggc aagtttgccg gtgtgattgt cgatttcagg accgatgcca
     3181 agggcgatgc ggtgctgttt gacttcaacc acccgctcgc gggccagccg gtcacatttg
     3241 aagtgcatgt gatcggggtg ttatgaccgg gttgcaaagc ggcgcggcga gcatcctgct
     3301 ggccgagcca cgcggctttt gtgccggggt ggaccgggcc atcgagattg tcgaaaaggc
     3361 tttggccaag tttggggcac cgatttacgt gcgccacgag atcgtgcaca acacctatgt
     3421 ggtgaacgac ctgaaggcca agggcgccat cttcatcgaa gacctggccg atgtgccgcc
     3481 cggcgccacc ctggtcttca gcgcgcacgg cgtgagccgc gcggtgcggg tcgaggccga
     3541 cgaccgcggc ttccaggtgt tcgacgccac ctgcccgctg gtcaccaagg tgcatgtcga
     3601 ggtggccaag ctcgccaagg aaggctacga attcatcatg atcggccaca agggccaccc
     3661 cgaggtggaa ggcaccatgg ggcagctgtc cgacggcatc tacctggtgg aagacgtggc
     3721 cgacgtggcg cgtgtgcaac cacgtggccg gcaactggcc gtggtgacac agaccacctt
     3781 gagcgtggac gatgcggccg ccatcctggc cgaggtcaag cgcctgttcc cgcatgtgcg
     3841 cgagcccaag aagcaggaca tctgctacgc cacccagaac cgccaggacg cggtgaaggt
     3901 catggcaccg caggtcgaca tcgtcatcgt ggtcggcagc cccaccagca gcaacagcaa
     3961 ccgcctgcgt gaactggccg agcgcctggg cacgccggcg cacatggtcg attcggccga
     4021 cgacctgcag ccggcctggt ttgccggcca cccgcgcgtg ggcctgaccg ccggtgcctc
     4081 ggcccccgac gtgctggtgc agcaggtgat caaccgcctc agggccctgg gcgcgatcag
     4141 cgtgcgccgc atggccggcg tggaagaaac cgtgcacttc cccttgccca agggcctggg
     4201 cgacaaatcg atgagcgagt ttgccgcgtc gcgatcgtga agcgccgttc aatcgaaccc
     4261 cctcgacatt gaacggcacc cacgcggtgc cgtttgcaca aagctgatcc gaaggaccca
     4321 acaccatgct ggacatcaac ctgcttcgcc gcgatttgcc ggccgtcatt gctgcgttgg
     4381 aagcgcgcaa gtcaccgcag cccttcctgg acgtggcgcg cttcaccgcg ctcgagaccg
     4441 accgcaagac cctgcaggtc cgcaccgaag agttgcaggc ccgccgcaat gccctgagca
     4501 agcagatcgg ccaggccaag ggcaagggcg aagacgcgtc ggcgctgatg gccgaggtgg
     4561 gcggcatcgc cgaagagatg aaggccggcg gcgagcggct ggaagccatc caggccgagc
     4621 tggccacgat gctgatgtcg gtgcccaacc tgcccgatgc cagcgtgcca gtgggcgccg
     4681 acgagcaggc caaccaggaa gtgcgccgtt ggggcacgcc gggcagctac ggctttgcgg
     4741 tgaaggacca cgtcgacctg ggcgcaccgc tggggctgga cttcgagacc ggcgccaagt
     4801 tgagcggcgc acgcttcagc ttcctcaagg gaccggccgc gcggctgcac cgtgcgctcg
     4861 cccagttcat gc
//
LOCUS       DAOSLL010000352         4861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15527_length_4861_cov_4.845402, whole genome shotgun sequence.
ACCESSION   DAOSLL010000352 DAOSLL010000000
VERSION     DAOSLL010000352.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4861
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15527_length_4861_cov_4.845402"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="PLB25_14475"
     CDS             <1..408
                     /locus_tag="PLB25_14475"
                     /EC_number="1.13.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class III extradiol ring-cleavage dioxygenase"
                     /protein_id="HOE42816.1"
                     /translation="ANVPVVQLSLHASLDPLRHLEVGRALEALRDQDVLIIGSGMSFH
                     NMRAYGNVQFAPISDAFDEWLTQAVESGAQERSDLLSNWERAPGARQCHPPRAEEHLI
                     PLMTVAGAAGNDRGRKLFSDRVMETTLSAFSFG"
     gene            complement(585..1262)
                     /locus_tag="PLB25_14480"
                     /pseudo
     CDS             complement(585..1262)
                     /locus_tag="PLB25_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
     gene            complement(1272..2189)
                     /locus_tag="PLB25_14485"
     CDS             complement(1272..2189)
                     /locus_tag="PLB25_14485"
                     /inference="COORDINATES: protein motif:HMM:NF026853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HOE42817.1"
                     /translation="MFGTTGDRRTLACLAPANTALAQELLAKFGKSPPTGLFLELTLP
                     VGSGRNKANVLAALRMIHAGGLHASCRRNNQGVLIPYQATNGGGYTLEALLGITPNGN
                     SEPDYLGWEIKGHSSNRVTLMTPEPNGGFYGERGVREFVEHYGHEVNDGGMYFTGGHK
                     VGVRCVATGMTLQVSGFDPAAQKLLDVGGAVRLVDAAGNEAASWAFAQLLTHWTRKHA
                     FAAYVRYTAQKMPIAYRYDTPVLMGEHTDFGKFLNALCSGAIVFDPGSKVTQARTTQS
                     KVKARSQFRIKTKDLGLLYQVLTAESIAG"
     gene            complement(2532..2855)
                     /locus_tag="PLB25_14490"
     CDS             complement(2532..2855)
                     /locus_tag="PLB25_14490"
                     /inference="COORDINATES: protein motif:HMM:NF024783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOE42818.1"
                     /translation="MSGLSDARLLIASHIVPWSSDKTNRLNPSNGLCLSAIHDRAFDK
                     GLMTLSDDLKIVISEVLKRSDDVFVRDVLMPLNGRMIESPERFTPQLEFVTWHRNSIF
                     VDNQK"
     gene            complement(3378..4586)
                     /gene="dcm"
                     /locus_tag="PLB25_14495"
                     /pseudo
     CDS             complement(3378..4586)
                     /gene="dcm"
                     /locus_tag="PLB25_14495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008648335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
BASE COUNT         1016 a         1403 c         1368 g         1074 t
ORIGIN      
        1 gcaaacgtgc cagtggtgca actgtcgctg cacgcgtcgc tggacccgct gcggcatctg
       61 gaggtcggcc gcgcgctgga ggccttgcgc gatcaggatg tgctgatcat cggcagcggc
      121 atgagctttc acaatatgcg cgcttatggc aatgtgcaat tcgccccgat atctgatgca
      181 tttgacgagt ggctgaccca agccgttgag tccggcgcgc aagagcgcag cgatttgctg
      241 tcgaactggg aacgcgcacc cggtgcccgg cagtgtcatc cgccacgggc cgaagaacac
      301 ctgattccgc tgatgacggt ggccggtgca gcgggcaatg atcggggtcg caagctgttc
      361 tcggatcgtg tgatggaaac gacactgtcg gccttcagct ttggctgaca catcgtcctg
      421 gttcgtccat ctgattccat ttccagatca tgcatgtcgt tgttatctcg ccttgccttg
      481 cgtttcacac cgcccaggtt tgtgcatcta agaggcctcc agcgcccgtc cgcattgcgc
      541 agcaagctat aaaaacaaaa gcatggcgca gtctgcctgt gggcctattg gcgcgtcgcc
      601 cgccattcct cctgcagcag cgccagcatg tcgtcagccg acatcctggc tgccaggtcg
      661 ctgccttcga gcaggctgtc ggccagggcg cgcttggtct tgtgcaaatc gacaatcttt
      721 tcctcaatcg tctgactggc caccagccgg tagatggtga ccgggcgggt ctggccgatg
      781 cggtgggcac ggtcgctggc ctggtcttcc acggcagggt tccaccatgg gtccatgtgg
      841 atcacatagt cggcggcggt caggttcagc ccggtgccac cggctttcag gctgatcagg
      901 aacacatcac cgtcgcccgc ctgaaacgca tcgacgcgca gcttgcgctc ctgcatcggg
      961 gtggcgccgt cgaggtattg gtaacgcaca ccacgcgcat cgagctcggc acgaatcagg
     1021 ctcaggtgat cgacaaactg gctgaacacc agcgccttgt ggcggttggc cagcaactcg
     1081 tcgagcagtt cgccaaaggc cgccagcttg ctgctgggca ggcccagcgc cggagccacg
     1141 agttgcgggt tgcagcaggc gcggcgcagt ttcatgatct cggccagtat ctggatatgg
     1201 cgttggccag cctgtgcctc ggagcctgcc aggcggtcga gggcctcgcg ccgcattgct
     1261 tttaatcgtg ttcacccggc aatactctct gccgtaagca cctgatacag cagccccaga
     1321 tccttggtct tgatgcggaa ctggcttctg gccttgactt ttgactgcgt cgtgcgggcc
     1381 tgcgtcactt tggagccggg gtcaaaaacg atggctcctg agcaaagtgc attcaggaat
     1441 ttgccaaaat cggtgtgttc gcccatcaga accggcgtgt cgtagcggta ggcaatgggc
     1501 attttttgtg ccgtgtagcg cacgtaggcg gcaaacgcgt gtttgcgagt ccaatgcgtc
     1561 aacaactgtg caaaggccca tgatgctgct tcattgccag cggcatccac cagccgcacg
     1621 gcaccaccca cgtccagcaa cttctgggct gccgggtcaa atccggacac ttgcagggtc
     1681 atgccggtgg ccacacaacg caccccgacc ttgtggccgc ccgtgaagta catgccgcca
     1741 tcgttcacct catggccgta atgctcgaca aattccctga cacccctttc gccgtaaaag
     1801 ccgccgttgg gctcgggggt catcaacgtg actcggtttg aactgtggcc ctttatctcc
     1861 cagcccaggt agtcaggctc ggaattgccg ttgggcgtga tgcccagcag ggcctcaagt
     1921 gtgtagccac cgccattggt ggcctgatag gggatgagta cgccctgatt gttcctgcgg
     1981 catgacgcgt gtaaaccgcc cgcatggatc atgcgaagcg ccgcaagaac gttggccttg
     2041 ttccggccag accccacggg gagcgtcagt tccaggaaaa ggccggtggg tggcgatttg
     2101 ccaaatttgg ccagcagttc ctgcgccagc gcggtattgg cgggcgcaag gcaggccaat
     2161 gtgcgcctgt cgccggttgt accaaacacg agcacccggc catcgatgcc acgccgctgt
     2221 tctttgggga cttgtcgcaa atgttccgat ggcgcggtct tgcagcctgt caaaactgct
     2281 taaattctga gcaaaatgag acttcggaag ttgttatgcg tcagcgtgct aattatccaa
     2341 gtcgtccatg tcatcctcga caactggaat actgcgttgg cacttcagac attcacaaac
     2401 aaagacgctc tcatcatcaa tactcactat ttcaagcagc atcaaccttg atccgcaact
     2461 gtggcaagta tcaggttgct ccgtcatgac ttgataaacg gtctctggcg gaaatttcca
     2521 tctcatggca gctacttctg gttgtccaca aatattgaat tgcgatgcca cgttacaaac
     2581 tcaagctgtg gcgtaaagcg ctcgggtgac tcaatcattc ggccattcaa tggcatcaaa
     2641 acatctctga caaacacgtc atcgctgcgc ttcaaaacct ccgaaatgac tattttcaaa
     2701 tcatcagaaa gtgtcatcaa tcctttatca aacgcacggt catggattgc agacagacaa
     2761 agtccattgc tcgggttgag cctattggtc ttatcgctgc tccacggcac gatatggctg
     2821 gcaattaaca agcgtgcatc tgacagaccc gacatacaac aacggcctcg ataactgctg
     2881 agtacggcac ggcggaaaaa atgctgtttg atgcgttgcg ttgttaaaac ctgccgggtt
     2941 tcccccgtga agtcatctgg caaaaacgcg tcattttcag agtccagctc tggcgcagaa
     3001 tttttgtcca attgttgacg cagcaactga cattccacgg ctaaacgctc ccaattcgag
     3061 tgaaattcgt cccatacgga tcggtctaac gcagaggcat ttcccaatcc ggcacgtcct
     3121 gtgcttgtaa tcgcagggtc aatgctggcg atgttgagca ttttcattgc cacggcatct
     3181 gacgtgcgtc caatgacttt cgccaaggcc atgatttctg ggttgcgccc atgaatacgt
     3241 ccatagggga gttggcaata caaatgaaaa gccagcttaa tttgctcttt ggtccaaagt
     3301 ccggttgcca tgatgtttgt cggcacgatg acggaggaaa ccgcttcgac cgattccttg
     3361 ggtaaaatga ttgaggcaat cagtttaccg accgcttgaa ccataggaac aaccgccgct
     3421 tcggagaaca agcggtaggc ttgagtatcc gacaccggaa tcctgaacgt gtccggaaac
     3481 cccatcaacc gggcgcattc acgcggtgtc aggcggcgcg ggttcttgtt ggcaccctgg
     3541 ctgaacagga tttccgaccc gtccttgtaa tagcgagctg acagcgtgcg cgttacgctc
     3601 tcgggcgtga ccagcccgaa gccgaaaccg ttgcccgctg ccgcatgctt ctttttgtag
     3661 ttttgcaggt acagccaaag gtggtcggtc agcgtgtact tgtccagcac cttgcggctt
     3721 gcatggtcgt agtagctgga accgtccggc tcaagccgtg gttcacctgc tttgcggtgg
     3781 atgatcccgg ccagcgtgtg gctgcctttt ggcggcaggt caaccatgcc ccagtcaaaa
     3841 tcagcgggct ggcggaagcc gacaatgatg atgcgttcac ggtgctgtgg cacaaagtgg
     3901 gcaccgtcga tcaccttgtg aaaaacctga tatcccagct cgtcacgcag ggtgcgcagg
     3961 atcacgtcaa aggtacggcc cttgtcatgc gactgcaggt ctttgacgtt ttcaagcatg
     4021 aaggccttgg gctgcttggc ggcaatgatg cgcgccacgt caaaaaacag cgtgccctgt
     4081 gtttcgtcag caaaaccgtg cgcccggcca agggcattct ttttggacac cccggcaatc
     4141 gaaaacggct ggcacggaaa cccgcccagc aaaacgtcat ggtcaggaat gtcagtggcg
     4201 gcaatccggg tgatgtcgcc atcaatcggg tgctgtccac caaaattggc catgtaggtg
     4261 cgctgggcgt agctgtccca ctcgctggta aacacgcatt cgccaccaat ggcatcgaac
     4321 gccagccgga tgccgccaat ccccgcaaac aggtcgatga atttgaagtc ggcaccggag
     4381 ttggtgacag gctggaatgg cagcaaccgt tgcagtgcta ggccgacata gaggggcggg
     4441 tgcgactcgc ccacttccca gcgcctgacc tggcgttcgc tgaccccaag gtgtttggca
     4501 atggcagctt gcgagtggtg ttggcgggct gctgcaaggt atgccagcgc ggcggtgtcg
     4561 ttcagggtgg ttgacagaat gttcataaaa gcaatcatac cggacataat gtccggtatt
     4621 tatccccctg ataaataaac agtattttgg ctatcccgaa gcgtcatgat gccaccaaaa
     4681 gcaacaagcc atttacccct cgaaccgccg tacctattgc gcaagacgtt acaacaagat
     4741 taccctatta acgctcaagc agaatcctgc ccggtcttgg cccgccattc ctcctgcagc
     4801 agcgcgagca tgtcgtcagc cgacatcctg gctgccaggt cgctgccttc gagcaggctg
     4861 t
//
LOCUS       DAOSLL010000353         4857 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15550_length_4857_cov_4.751354, whole genome shotgun sequence.
ACCESSION   DAOSLL010000353 DAOSLL010000000
VERSION     DAOSLL010000353.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15550_length_4857_cov_4.751354"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="PLB25_14500"
     CDS             complement(<1..323)
                     /locus_tag="PLB25_14500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HOE42819.1"
                     /translation="MTASPLLIPAPLGALLGRLPAYPGSLLFVTGLNLALAKQLAPDV
                     TQMLLGKKLRLRVLDAQWAFDFEWKDDRFVACQNPAAADLTISASAHDFVLLARRQED
                     PDTLF"
     gene            complement(351..1247)
                     /locus_tag="PLB25_14505"
     CDS             complement(351..1247)
                     /locus_tag="PLB25_14505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOE42820.1"
                     /translation="MKLSLGPLLYYWPRDQVLAFYASVASSPVDIVYLGEAVCSRRHE
                     VRLADWLEIAQTLHQAGKEVVLSTQVLIESGADVAVLHKIADNGLFLVEANDMGAVHC
                     LSGKAPFVAGPHLNIYNLPTLQWMAPLGIRRWVMPLEMGREELAVMQSGRPEGVQTEV
                     FAFGRLPLAFSARCFTARHANCPKDDCQFKCLAYPDGLMMRTRENEEFLVLNGIQTQS
                     ARVYNLLPELAAMSELGVDVVRISPQSSHTLDIVKLFGEVLAQRMPVATALQSLQPLM
                     LEKACNGYWYGKPGLELVAASA"
     gene            complement(1507..2565)
                     /locus_tag="PLB25_14510"
     CDS             complement(1507..2565)
                     /locus_tag="PLB25_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HOE42821.1"
                     /translation="MNFVADSVLARPPAQTPAAPMELVCPAGSLPALKAAVDHGADCV
                     YLGFRDATNARNFAGLNFDAAAVETGIRYAHDRGRKVFVALNTYPQATKAELWQRALD
                     RAADSGVDAVILADPGLMAYAVRHQPQLRLHLSVQGSATNYEALDFYHRHFGIARAVL
                     PRVLSMLQVEQVIAKAGIEIEVFGFGSLCVMVEGRCALSSYVTGEAPNTNGVCSPPKA
                     VRWVETPQGRESRLNGVLIDRYAPGENAGYPTLCKGRFDVEDDQNYYAIEEPTSLNTL
                     SLLPELMAMGVRAIKIEGRQRSPAYVAQVTKVWRAAIDNCRSDPQRFTAKPAWISDLD
                     KVAEGQQHTLGAYHRPWK"
     gene            2830..4254
                     /locus_tag="PLB25_14515"
     CDS             2830..4254
                     /locus_tag="PLB25_14515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HOE42822.1"
                     /translation="MTTPTAQTSLLPECHPRAVPEALLDALGQRFTARFSTARVIREQ
                     HGRDESSFSVPPPDAVVFAQSTQDVADAVKLAAQYKVPVIPFGIGSSLEGQLLAVQGG
                     ISLDVSRMDQVLSINAEDLTVTVQPGVTRKQVNEAVKSTGLFFPIDPGADATIGGMSA
                     TRASGTNAVRYGTMRENVLALEVVTAQGSIIRTGTRARKSAAGYDLTRLMVGSEGTLG
                     VITEVTVKLYPLPEAVMAATCSFASLADAVNATIQIIQLGVPIARCELLDANTIRMVN
                     KHSRLSLNEAAMLLMEFHGSPAGVREQVETVQAIAAEWGGQAFEWANTPEERTRLWTA
                     RHNAYFAGVQSRPGCKAITTDTCVPISSLADALLDSVSEAEQSGLTCFLVGHVGDGNF
                     HMGYLIDPTVPHERETAERLNRQLVQRALKLGGTCTGEHGVGLHKMGFLVDETGAGAV
                     AMMRAIKLALDPDNIMNPGKIFLL"
BASE COUNT         1013 a         1451 c         1452 g          941 t
ORIGIN      
        1 aaaaacagcg tgtcggggtc ttcctgcctg cgcgccagca gtacaaagtc atgcgcactg
       61 gcgctgatgg tcaggtcggc ggcagccggg ttctggcaag cgacaaaacg atcatctttc
      121 cattcaaaat caaaggccca ttgcgcgtca agcacccgca ggcgaagttt tttgccaagc
      181 agcatttgcg tgacatccgg ggccagttgc ttggccaggg cgaggttgag cccggtcaca
      241 aacagcaatg agcctgggta ggcgggcagt cggcccagca acgcgccaag cggggccgga
      301 atcagcagtg ggctagcagt catggggaga tccttgtttg aacaggttgg tcaggcactg
      361 gctgcaacca gctccaggcc tggtttgccg taccagtaac cgttgcaggc tttttccagc
      421 atcaacggct gcagggactg cagtgcagtt gcgaccggca tgcgctgtgc cagcacttcg
      481 ccgaacagtt tgacaatgtc aagcgtatgg ctcgattgcg ggctgatgcg caccacatca
      541 acccccaatt cactcatggc cgcaagctcg ggcagcaggt tgtacacgcg ggcagactgg
      601 gtttgaatgc cattgagcac cagaaactct tcgttctcgc gggttcgcat catcagtccg
      661 tccggatagg ccagacactt gaactgacag tcgtccttgg ggcaattggc gtgacgggcg
      721 gtaaagcagc gcgccgagaa ggccagcggc aggcggccaa aggcaaacac ttcggtttgc
      781 acgccttcag gtcggccgct ttgcattacc gccagttcct cgcggcccat ctccagcggc
      841 atgacccagc gccggatgcc tagcggcgcc atccattgca gggtcggcag gttatagatg
      901 ttgaggtgcg gcccagccac aaaaggggct ttgcccgaca ggcaatgcac cgcgcccatg
      961 tcgttggctt ccaccagaaa caagccgttg tcggcaattt tgtgcagcac cgcgacatcg
     1021 gcaccggact cgatcagcac ctgggttgac agcaccacct ctttgcctgc ctggtgcagg
     1081 gtttgcgcaa tctccagcca gtcggccagg cgaacttcat ggcggcgcga gcagaccgcc
     1141 tcacccaaat aaacgatgtc caccggactg ctggcgaccg atgcgtaaaa agccagcacc
     1201 tggtcacgtg gccagtaata caacagtgga ccgagagaca gtttcacaaa gcatcctcta
     1261 aatggttggg ggcaacgggt atccgacgac aggtcgggaa cagagcatca gttcgcttcg
     1321 cgtaacctgt ggtctgtccc gcaaggccga cagccaacat ccgacgaaat gttggcgaca
     1381 gagcacatgt tcgcttcgcg taacctgtgg tctgtcccgc aaggtgggca gccaacatcc
     1441 gacgaaatgt tggcgacaga gcacatgttc gcttcgcgta acctgtggtc tgtcccgcaa
     1501 ggttatttat ttccatggtc gatggtaggc gccaagcgtg tgttgctggc cttcggctac
     1561 tttgtcgagg tcgctgatcc aggctggttt ggctgtaaag cgttgcgggt cactgcggca
     1621 attgtcaatg gcggcgcgcc agaccttggt gacctgtgcc acatacgccg ggctgcgctg
     1681 acggccttcg atcttgatgg cgcgcacacc catggccatc agttcgggta acagcgacag
     1741 cgtgttgagg ctggttggct cttcaatggc gtagtagttc tggtcgtctt ccacgtcaaa
     1801 acgacccttg cacagggttg ggtaaccggc gttttcgcct ggcgcgtaac ggtcaatcag
     1861 cacaccgttc aggcgcgact cgcggccctg cggggtttcc acccagcgca ccgccttcgg
     1921 cggcgaacag accccgttgg tgttcggggc ttcgccggtg acgtagctcg acagcgcgca
     1981 gcgcccttcc accatcacgc acaggctgcc aaagccaaac acctcgatct cgatgccagc
     2041 cttggcaatc acctgctcga cctgcagcat tgacagcacc cgcggcaaca ctgctcgcgc
     2101 aatgccaaag tgccggtgat aaaaatccag cgcctcgtaa ttggtggctg agccctgcac
     2161 cgacaagtgc aggcgcagtt gcggctggtg gcgaacggcg taggccatca gacccggatc
     2221 ggccagaatc acggcatcaa caccgctgtc ggctgcacgg tccagcgcac gttgccagag
     2281 ctcggccttg gtcgcctgcg ggtaggtgtt aagcgccaca aacaccttgc ggccacggtc
     2341 atgcgcgtag cggatgccgg tttcgacggc tgccgcatca aaattcagac cggcaaaatt
     2401 gcgcgcattg gtggcatcac gaaaacccag ataaacgcaa tcggcaccgt ggtccacggc
     2461 ggctttgagg gcaggcaggc tgccagccgg gcagaccagt tccatcgggg ctgcgggtgt
     2521 ttgcgctggc gggcgggcca ggacggaatc agcgacgaaa ttcataaaag tctggtgaag
     2581 ctttggaaaa tgcttgtcat ggtaaacccg taagcctaac cagtccggtc gtccggttaa
     2641 atgatgtata tcaagcaaat gccaataaaa ggcagggcaa gggcgctacc agacactgcc
     2701 gtcactttga tgttgctggt gcggcacatc gtcccctgcg ggcaaccccg ggccttttcg
     2761 aacagaggca aattcccgcc aggaacaggc gctattgcgc ataattgccg ctcaccccgg
     2821 aagtccgcca tgaccacacc caccgcccaa accagcttgc tgcccgaatg tcacccccgt
     2881 gccgtacccg aggccttgct ggacgcgtta ggccagcgtt tcacggcgcg tttctctacc
     2941 gcgcgggtga tccgcgagca gcatgggcgt gacgagtcct cgttcagcgt gccgccgccc
     3001 gacgcggtgg tgtttgccca aagcacccag gacgtggccg atgccgtcaa actcgcagcg
     3061 cagtacaaag tgccggtgat tccgtttggc attggatcgt ctctggaggg ccagttgctg
     3121 gcagtgcaag gtggcatcag cctggatgtc agccggatgg accaggtgct cagcatcaac
     3181 gccgaagacc tgacggtgac ggtgcagcct ggcgtgacgc gcaaacaggt caatgaagcc
     3241 gtcaagtcga ccggcctgtt ctttccgatt gaccctgggg ccgatgccac gataggcggc
     3301 atgagtgcca cacgcgccag cggcaccaac gccgtgcgct acggcaccat gcgcgaaaac
     3361 gtgttggcac ttgaagtggt gacagcacag ggcagcatca ttcgcaccgg cacccgagcc
     3421 agaaagtcgg ccgctggcta cgacctgacg cgcctgatgg tcggcagcga gggcacgctc
     3481 ggtgtcatca ccgaggtcac tgtcaagctg tacccgctac ccgaagcggt gatggccgct
     3541 acctgctcgt ttgccagcct ggccgatgct gtcaacgcca ccatccagat cattcagttg
     3601 ggcgtgccaa tcgcccgctg cgaactgctt gatgccaata ccatccgcat ggtcaacaag
     3661 cattcgcgcc taagcctgaa cgaagcagcc atgttgctga tggagtttca cggctcgcct
     3721 gccggggtgc gggagcaggt cgaaacggtg caggccatcg ccgccgaatg gggcggtcag
     3781 gcctttgagt gggccaacac ccctgaagaa cgcacccggc tgtggaccgc acggcacaac
     3841 gcctactttg ctggcgtgca gtcgcgccct ggctgcaaag ccatcaccac cgacacctgt
     3901 gtgcccatat caagcctggc cgatgcgctg ctggactcgg tgtccgaggc cgagcaaagt
     3961 ggtctgacct gttttctggt cggccatgtg ggcgacggca actttcacat gggttacctg
     4021 attgacccga ccgtgccgca tgagcgcgag accgccgagc gcctcaaccg gcaactggtg
     4081 cagcgagccc tgaaactggg tggcacctgc accggcgaac atggcgtagg gctgcacaag
     4141 atgggatttc tggtcgacga gaccggtgcc ggtgcggtcg ccatgatgcg cgccatcaaa
     4201 ctggcactgg acccggacaa catcatgaat ccggggaaga tatttttgct gtagcagcct
     4261 gacgggcttg aaagtctttt ggctctgttg aagtaaattc ctcagttgaa cgcgcccgag
     4321 tgggtcaccg cgcgacgcgg ctgagtagca cgacgacgac gctcagcttc tgcccgccag
     4381 caggagtagc gaccccaggc acgttgccag cggcccactc gggcgcgtcc aactcaggga
     4441 tcaaggttca aggttcaagg cagcaattaa cccgccatgc tgtgaagcgt acgggttttg
     4501 ggggtgtttt ggcgaattgt gaaaacatct gaaacgatgt cctggtgccc gagccggaac
     4561 cgcgtttaga atttaagttt ttgattcaat tcaataaaaa cgtttctaaa aaaataagta
     4621 ccgtcaaaag taccgtcatt tttcaatccg tctcattttt gctacaccgt gtcacgtgct
     4681 ctgacatttt gcgcagatca gcgaaagcaa acctgcataa ccgacataac ccatcgctgg
     4741 ggtttgggtt gctgctttgg gcgatgcagc caagattgca tcaagtggtg tgtgccagtc
     4801 gcatatcggc ctgataccac cagcgccggt atcactgcca ccagaacggc tcagggt
//
LOCUS       DAOSLL010000354         4855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15559_length_4855_cov_4.209792, whole genome shotgun sequence.
ACCESSION   DAOSLL010000354 DAOSLL010000000
VERSION     DAOSLL010000354.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15559_length_4855_cov_4.209792"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1557)
                     /locus_tag="PLB25_14520"
     CDS             complement(<1..1557)
                     /locus_tag="PLB25_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42823.1"
                     /translation="METDASDKTYCVSNGEFLLAVFGKAMTDALAEARPVLVSFDGNP
                     ATAPAKVWSGKPWLGDADLSTSLPASANNYFSLAVFRPDEAGQFRRLKARFMALYAVM
                     LDDVGTKVAMERLTLPPTWLLETSVGNFQAGYLLSEPLADGPAADRLMNAIIAAGLCD
                     PGANGPRARLARLPVAMNGKHAPPFACRMAGWEPDFCYSVAELVGGLELELAAETRPK
                     RQSARAAQQRPSDGDPVWLPRPEENSVLIALHDRGLYKAPLGDGKHNITCPWVQEHTG
                     EVNGGTAYFEPDDNWPIGGFKCLHGHCAERHVRDLLQFVGVEVSAARMKSTIRVMAGE
                     IHRVVDAAERELAQSRRHYQRGGLIVTIVTDPGTRETRVQDISPPALVRALAGAATWE
                     RFDARAEDWCRTDPPARHAAVLFDSTSYRHLPVLNGLTRQPYLRPDGSLMLAAGYDPA
                     TGMFGVFDSREFSVPQNPTRDDALAALALLKDLLAEFSFARDADLSAALAGLLTAAVR
                     PSLNHSPMIHV"
     gene            complement(1604..1846)
                     /locus_tag="PLB25_14525"
     CDS             complement(1604..1846)
                     /locus_tag="PLB25_14525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HOE42824.1"
                     /translation="MTHTILRIPATKSQTGYSRSTIYLRITQGLWTKQISLGPRAVGW
                     PATEVAALNAARISGKTDAEIRELVVKLEAARKAAI"
     gene            complement(2098..3576)
                     /locus_tag="PLB25_14530"
     CDS             complement(2098..3576)
                     /locus_tag="PLB25_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42825.1"
                     /translation="MLRAKSNRVRANERELFSEFSKGHYADAHWRLIERDDGRYVEAH
                     DFLEWLSQYVAAQTQPSIAFPQDLIHAVREATSASFAQSKTPVIFESLTIALEGSFDQ
                     SLAGLPIALRQRVEQEFFPMPWDTLSAEQRRSVALQMDYQHDPATEKDRKFWWDFFER
                     VDNLKKQRAEWEVVATPTAAELALKETRLAELKQELARMDAQARLARGDCYPERRPSQ
                     NKDGDTVAEPATATRYMAYPKAIHQLTARLGATPEELAAWIWLGPTDGGIAAYMNANE
                     LDPPPRFFYVTGSNSHDYIAPLMACWFKVEDIDQFVPTDRYITGAALMERWGKRPGLH
                     AVAFIQAKIAESRLLDIHPIYGGTRGTFSEHSDWPPLESGLFPLAHIERIEVEDFAET
                     SPVENLPPGTPAQPTKHQPGDEQQRTHADTGEANRARGSTVRRDARKLVTQATYKLWQ
                     KAYRATKKTHPNKSDIWYSRHIAKLKIAKGRDAETIRKHMKL"
     gene            3928..>4855
                     /locus_tag="PLB25_14535"
     CDS             3928..>4855
                     /locus_tag="PLB25_14535"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOE42826.1"
                     /translation="MARSTVHYADKETNNILKKLHNKLRPAGTPVQRVEYIIELLLLR
                     IFEVKLGQDPEYTRLRGAFAGANETLLFTALLSLPNDQILAALNTRFFPFYARILSEA
                     RKVWQGNLSTKVQDQLILIEEVFGNSNFTNNVTSGNMAEIIGLVAELDSERLLKTDLL
                     GDAIESALSETGGTKDIGLHRTPDHIRQFMVALAAPTFKSRLFDPAVGTGGFMFDSFE
                     YVLEGIYREGHWPGPKAHPELAAWFKDWFERHPTELPSHEVSTDFYRTGVGGIEYLGM
                     VRKMASINFFVRGLNPGTIQQGDSLEKFGTDIL"
BASE COUNT         1041 a         1431 c         1336 g         1047 t
ORIGIN      
        1 cacatggatc attggcgagt ggttcaggct gggtcgcacc gctgcagtca gcaggccagc
       61 cagcgccgcc gacagatcag catcgcgcgc aaagctgaat tcggccagca ggtctttcag
      121 caacgccagc gccgcaagcg cgtcgtcacg ggttggattt tgagggactg agaattcacg
      181 cgaatcaaac accccaaaca tgccggtggc cgggtcatag cctgctgcca gcatcagact
      241 gccatccggt cgcaagtaag gctggcgagt caggccattg agcaccggaa gatggcggta
      301 actggtggag tcaaacagca ccgccgcatg gcgcgccggt gggtcagtgc ggcaccaatc
      361 ttcagcgcgg gcatcaaaac gttcccaggt ggcagcccca gccaaggcac gcaccagcgc
      421 cggggggctg atgtcttgca cacgggtctc gcgcgtacca gggtcggtga cgatggtcac
      481 gatcaaccca cctcgctggt agtggcggcg cgattgcgcc agttcgcgtt cagcggcatc
      541 caccacgcgg tgaatttccc ccgccatcac gcgaatcgtg gacttcatgc gggcggcgct
      601 gacctcgaca ccgacaaatt gcagcaggtc gcggacatgc cgctcggcac aatgcccgtg
      661 cagacatttg aacccgccaa tcggccagtt gtcatcgggt tcaaaatatg ccgtgccacc
      721 attgacctcg ccggtgtgct cttgcaccca tgggcaggtg atgttgtgct tgccatcgcc
      781 cagcggtgcc ttgtaaaggc cacggtcatg caatgcgatc aatacgctgt tttcttcagg
      841 gcgaggtagc cagaccgggt caccatcgct ggggcgctgt tgcgcagcac gggcactttg
      901 ccgcttggga cgcgtctcgg cggccaactc cagctcaagg ccaccgacca gttcggcgac
      961 cgagtagcaa aaatcaggtt cccaccctgc cattcggcaa gcaaacggcg gcgcgtgttt
     1021 gccgttcatg gcaaccggca gccgggccag ccgcgctctt gggccatttg caccggggtc
     1081 acacaatccg gcagcgatga tggcgttcat cagccgatcc gcagcagggc catctgcgag
     1141 cggttcactc aacaaatatc cggcctgaaa attgccgacc gaagtctcca gcaaccaggt
     1201 tggtggcaag gtcagccgct ccatcgccac cttggtaccc acatcgtcca gcatcaccgc
     1261 atacagagcc atgaagcgcg ctttcagccg ccgaaactgg ccagcctcgt ccggcctgaa
     1321 aacggccaaa ctaaagtaat tgttggcact ggcgggcaaa ctggtggaca gatcggcatc
     1381 acctagccac ggcttgccag accagacctt ggcaggtgct gtggcagggt tgccatcgaa
     1441 gctgaccagc accggacgcg cctcggccaa tgcatcggtc atggccttgc caaacacagc
     1501 cagcaaaaac tctccattgc tgacacagta ggttttgtcg cttgcgtcgg tttccacggg
     1561 gtcaggtgca aaacctgtca ggggcgtgtt caacgtcgcc accttaaatc gcggccttgc
     1621 gggccgcttc cagcttcacc accagttcac ggatttcggc atccgtttta cccgagatgc
     1681 gtgccgcatt cagcgcagca acttcagtgg caggccaacc gaccgcgcgt ggccccagac
     1741 tgatttgttt tgtccacaag ccctgtgtga tgcgaaggta aatcgtggag cgggaatagc
     1801 ccgtttgcga tttggtagcc gggatgcgta ggatggtatg cgtcattttt agtcctcttt
     1861 gacggttaag gaacgatgtc agacgcaagg ttatttgggt gatttgggat atttgggcga
     1921 aacaaaccgt tccgatctga tggctttcag agggaaaatt actgtgggag tggtcgcgtg
     1981 gcgcaagtcc tgaattcagt gtcgtgatcg aactgaatca ctccgcaccc gaaaagacca
     2041 gccggtcctg ggtgcaaaag ttgggtgaat agggcgaaaa gttttcgccc aactatttca
     2101 caatttcatg tgttttctaa tggtctcggc atcacgccct tttgctattt tcaactttgc
     2161 gatgtgccgt gagtaccaaa tgtccgattt attcggatgg gtctttttgg tggcgcggta
     2221 cgccttttgc caaagcttgt aggtggcttg tgtgactagt ttgcgtgcgt cccgtcgcac
     2281 tgtagaacct cgcgccctat ttgcttcgcc ggtgtctgca tgggttcgct gttgctcgtc
     2341 gcccggctga tgtttagttg gttgagcagg ggtgccgggg ggcaggtttt caactggact
     2401 tgtctctgca aaatcctcaa cttcaatccg ctcaatatgc gctagcggaa acaagccact
     2461 ttccagcggt ggccaatcgg aatgctctga aaaagtgccc cgtgtgccgc catatattgg
     2521 gtgaatatcc aagagccggg attcggctat ttttgcctgg atgaatgcaa ccgcgtgtaa
     2581 accaggccgc ttgccccatc gctccatcaa cgctgcacca gtgatgtagc ggtctgtagg
     2641 cacaaactgg tcaatgtctt ccaccttgaa ccagcatgcc atcaacggcg caatgtaatc
     2701 atggctgttg ctaccggtca catagaaaaa acgaggcggt gggtccagct cattggcatt
     2761 catgtacgcg gcaattccgc cgtctgtcgg tccaagccat atccaggctg ccaattcctc
     2821 tggggttgcg ccaagccgcg cagtcaactg gtgtatggct ttgggatacg ccatataccg
     2881 ggtcgcagtg gctggctcag cgacagtatc gccatccttg ttctgacttg gtcttcgttc
     2941 cgggtagcaa tcgcctcgcg ccagcctcgc ctgcgcgtcc atgcgggcaa gttcctgttt
     3001 aagttccgca agtcgggttt ctttaagcgc cagctcagcg gcggtcgggg tggcaaccac
     3061 ctcccactcg gcacgctgct tcttcaggtt gtcaacccgc tcaaagaaat cccaccaaaa
     3121 ttttcgatcc ttctcggtag ctggatcatg ctggtagtcc atttgaagcg ccacactgcg
     3181 ccgctgctcc gcagacagcg tgtcccacgg catggggaaa aactcttgtt caacccgctg
     3241 acgcagtgcg attggcaggc cagcgagtga ttggtcaaat gatccctcca gcgcaatcgt
     3301 caggctctcg aaaatcaccg gggttttcga ttgcgcaaac gatgccgagg ttgcctctct
     3361 tactgcatgt atcaggtctt gcgggaatgc gattgaaggc tgcgtctggg ctgccacata
     3421 ctggctcagc cattccagaa aatcgtgcgc ctcaacgtaa cgaccatcgt cgcgctcaat
     3481 cagtcgccaa tgagcgtcgg cgtaatggcc tttcgagaat tcgctaaaca actctctttc
     3541 gttggcacga actcgattgc tcttggcgcg aagcacgggt actaaatttc gatcagcatc
     3601 gcaaactggt aggtagcctg ccccaagcct tatcgcgtca gccaaggacc accagatcgt
     3661 tccatcttgc agaacattca gaaatggaga gtagtcaatt tccacgattc acccttggcc
     3721 ggtcttcctc aacacgtccc agtgccaacg gcagcaactc gtttcacaat tcacactaaa
     3781 agccatcgga cgactctgaa ccttgctgta cagtgcagta tagccacggc atgagtggca
     3841 tcgagatcaa accagccgca tttgcaaaga ctgtcacaca gaaggctgag ctggtgtctt
     3901 agaacccaaa gaaagtacac ccaccccatg gcgcgctcca ccgtccacta tgcagacaag
     3961 gaaaccaaca acatcctcaa aaaactgcac aacaagctgc gccctgccgg cacacccgta
     4021 cagcgggtgg agtacatcat cgaactgctg ttgctgcgca ttttcgaggt caagctaggg
     4081 caagaccctg aatacacccg gttacgcggc gcgttcgccg gggccaacga gacgctgctg
     4141 tttaccgcct tgttgagttt gcctaacgac cagattctgg cggcgctcaa cacccgtttt
     4201 ttcccgttct atgcccgcat cctgagcgag gcacgcaaag tctggcaggg caatctttcg
     4261 accaaggtgc aggatcaact gatcctgatc gaggaggttt ttggcaattc caacttcacc
     4321 aacaacgtca ccagcggcaa catggccgaa atcatcggtc tggtggccga actcgacagc
     4381 gaacggctgt tgaaaaccga cctgctgggc gatgccattg aatcggcctt gtcggaaacc
     4441 ggcggcacca aagacatcgg cctgcatcgg accccggatc acatccggca atttatggtc
     4501 gctctggcag caccgacctt caaatcgcgg ctttttgacc cggcggtggg taccggcggc
     4561 ttcatgtttg acagctttga atatgtgctg gaaggcattt accgcgaagg ccactggccc
     4621 ggcccaaaag cccacccaga actggcggcc tggttcaaag actggtttga gcggcatccg
     4681 acagaactac cctcgcacga agtcagcacc gacttttacc gtactggcgt gggtggcatc
     4741 gaatacctgg gcatggtgcg caaaatggcc tccatcaatt tctttgtgcg cggcctcaac
     4801 cccggcacca ttcagcaagg cgattcgctg gagaagttcg gcaccgacat tctgc
//
LOCUS       DAOSLL010000355         4851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15571_length_4851_cov_4.119266, whole genome shotgun sequence.
ACCESSION   DAOSLL010000355 DAOSLL010000000
VERSION     DAOSLL010000355.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15571_length_4851_cov_4.119266"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..480)
                     /locus_tag="PLB25_14540"
     CDS             complement(<1..480)
                     /locus_tag="PLB25_14540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42827.1"
                     /translation="MKTTFCALALVFLTSACSSLLPRSHSESTSFASFAQAREAIEQL
                     VPMKSDKSMLEKNGFNPATHPNTKILTHSDVVRLFVPTTLLKRDDLDPGILACLQAGD
                     ACHGMEIVGARIDRVRTGNFLADFSNFHRRTETTGWRFNALILFVNDLVVHRSWGGQP
                     "
     gene            complement(745..2052)
                     /gene="preA"
                     /locus_tag="PLB25_14545"
     CDS             complement(745..2052)
                     /gene="preA"
                     /locus_tag="PLB25_14545"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HOE42828.1"
                     /translation="MANLKTTFIGISSPNPFWLASAPPTDKAYNVNRAFEAGWGGVVW
                     KTLGEAGPSVVNVNGPRYGALLTPDRRLTGFNNIELITDRDLELNLKEIAEVKRLWPD
                     RAMVVSLMVPCVEDAWKAILPRVEDTGCDGIELNFGCPHGMSERGMGAAVGQVPEYIQ
                     MVTAWCKRYSKLPVIVKLTPNITDIRLPARAALAGGADAVSLINTINSIMGVDPYSLT
                     MSPSTGSKGSHGGYCGPAVKPIALNMVAEIARDPLTAGLPISGIGGIGNWRDAFDFIA
                     LGAGNVQVCTAAMVYGFKIVQEMADGLSNYMDAMGFSSIEDFRGKAIPSVADWRYLNL
                     NHISKAVINQDTCISCGRCHIACEDTSHQAIMAVKDGQRHFEVMEDACVGCNLCTLVC
                     PVPECITLRTVAPGELDLRTGKVVSPEHADWTTHPNNPACKAA"
     gene            complement(2163..3518)
                     /locus_tag="PLB25_14550"
     CDS             complement(2163..3518)
                     /locus_tag="PLB25_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE42829.1"
                     /translation="MSLSIAPDIRPGRLDAAEYARRFADATPRLTGPQAVLEAERCLY
                     CFDAPCATACPTHIDVPSFIRRIADGNLRGSAAAILQANPLGGMCARVCPTENLCEVV
                     CVRNTQEGKPVAIGRLQRHAVDALMESTQPQIFTRAAATGKKVAVVGAGPAGLACAHT
                     LARLGHQVVIFDAKPKAGGLNEYGLASYKTPNNFAQAEVQWLLGLGGIELRHGWKLQT
                     VAQLEALRQDFDALFLGMGLPNTHQLGIPGEALYGVQDAVDFIAILRQAEDLSKLPIG
                     RQVVVVGGGMTAIDAAVQAKLLGAQDVQMVYRRGPEAMTASKAEQEWAQTHGVVIHHW
                     LAPTDIMANSFDPTYAGGVRFARQKMLGGKLVPTGETLVIEADMVLKAIGQTLGNPLL
                     AQADFELQNGRIATDAHGQTSQRGVWAGGDCRAGGLDLTVEAVAHGTVSAQAIHAHLT
                     S"
     gene            3680..3805
                     /locus_tag="PLB25_14555"
                     /pseudo
     CDS             3680..3805
                     /locus_tag="PLB25_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
     gene            complement(4528..4782)
                     /locus_tag="PLB25_14560"
     CDS             complement(4528..4782)
                     /locus_tag="PLB25_14560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42830.1"
                     /translation="MRYLHIIILSAAGSIFNGTVHNIVYVWQDKVKLDDFFAHAAAMV
                     VGDFLGCFIVIMFFNACIDLAYKVVSRKRGTVQADSTTAR"
BASE COUNT         1036 a         1420 c         1462 g          933 t
ORIGIN      
        1 gggttgccca ccccatgagc gatggaccac caggtcgttg acaaacagga tcaacgcatt
       61 gaagcgccag ccggtggtct cggtgcgccg atgaaaattg ctgaagtcgg ccagaaaatt
      121 gccggtccgg acccggtcga ttcttgcgcc gacaatctcc atcccatggc aggcatcacc
      181 ggcttgcagg caagcgagga tgccggggtc aaggtcgtcg cgcttaagga gtgtggtggg
      241 cacaaagagg cgcaccacat ccgagtgcgt cagaattttg gtgttgggat gggtggccgg
      301 attgaagcca tttttctcca gcatgctttt gtcacttttc atcggtacca gttgttcaat
      361 cgcctcacgc gcctgggcaa aactggcaaa ggaagtggac tcggagtgcg acctgggcag
      421 caaggaagag caggcactgg tcagaaaaac caatgcaagt gcgcaaaatg tggttttcat
      481 gcgtcaagca tagcgcaatg ccagtcgaag catttgcgca aacgcaaaca acagaaagtt
      541 cctggcccct gccgacaagc ccttcgcatc gaccagccag tgccatccca agcggcccag
      601 ccttgatgca aaattggcct ccagccctac atcagcaagc gctaccagct acaaattatg
      661 cagcaaacta tagcctcgga aacttgcaac agcggacaac ggcgagcagc ggcgatgccc
      721 gacaggcttt attgcttgcg gcgatcaggc cgctttgcac gccgggttgt tggggtgcgt
      781 ggtccagtcg gcgtgctcgg ggctgaccac tttgccggtg cgcaagtcaa gctcgccggg
      841 cgcaacggtg cgcagcgtga tgcactcggg caccgggcag accagcgtgc acaggttgca
      901 gccgacacat gcgtcttcca tcacctcaaa gtggcgctgg ccatctttca ccgccatgat
      961 ggcctggtgc gaagtgtctt cgcaggcaat gtggcagcgt ccgcaggaaa tacaggtgtc
     1021 ctggttgatg accgccttgc tgatgtggtt gaggttgagg taacgccagt ccgccacgct
     1081 cgggatggcc ttgccacgga aatcttcgat cgacgaaaag cccatggcat ccatgtaatt
     1141 tgacaaaccg tcggccattt cctgcacgat tttgaagccg tagaccatcg ctgcggtaca
     1201 cacctgcacg ttgccggcac caagggcaat gaaatcaaac gcatcgcgcc agttgccgat
     1261 gccgccgatg cctgatatcg gcaagcctgc ggtgagcggg tcgcgcgcaa tctcggccac
     1321 catgttaagt gcaatcggct tcaccgccgg gccgcagtag ccgccgtgtg aacctttgct
     1381 gccagtcgat gggctcatgg tcaggctgta ggggtccacg cccatgatcg aattgatggt
     1441 gttgatcaga ctcaccgcat cggccccacc cgccagtgcc gcgcgcgctg gcaggcggat
     1501 gtcggtgatg ttgggggtga gcttgacgat caccggcagt ttgctgtagc gcttgcacca
     1561 ggccgtgacc atctggatgt attcgggcac ctggcccacg gccgcgccca tgccgcgctc
     1621 gctcatgccg tgcgggcagc caaagttgag ttcgatgccg tcacacccgg tgtcttcgac
     1681 gcgcggcaag atggccttcc aggcgtcttc aacgcagggc accatcagcg acaccaccat
     1741 ggcacggtcc ggccaaaggc gcttgacctc ggcgatctcc ttcaaattga gttccagatc
     1801 acggtcggtg atgagttcga tgttgttaaa gccggtcagg cgccggtctg gcgtgagcag
     1861 cgcgccatag cgcgggccgt tcacgttgac caccgatggt ccggcttcgc ccagcgtttt
     1921 ccagaccaca ccgccccaac cggcttcaaa ggcgcggttg acgttgtaag ccttgtcggt
     1981 gggtggagcc gaggccagcc agaacgggtt ggggctggag atgccaataa aggtggtttt
     2041 caggttcgcc atggtgtgct ttcaaatctt gggggcgctg cgtttggctg ggtgctctgg
     2101 cgctgcaaag ctgactcgca atcaatgaac ggcggatcag cgccgcaacg gcaagcgcgg
     2161 tttcatgaag tgagatgcgc gtgaatcgcc tgcgccgaca cggtgccatg ggccaccgcc
     2221 tcaaccgtca ggtcgagccc gccagcgcgg caatcaccgc cggcccagac accgcgctgg
     2281 ctggtctgac cgtgggcatc ggtggcaatg cggccatttt gcagttcaaa gtcagcctgc
     2341 gccagcaacg ggttgccaag cgtctggcca atcgccttga gcaccatgtc ggcctcgatc
     2401 accagggttt cgccagtcgg taccagcttg ccgccgagca tcttttgcct ggcaaagcgc
     2461 acgccaccgg cgtatgtcgg atcaaagctg ttggccatga tgtcagtcgg cgcaagccag
     2521 tggtggatca ccacgccatg ggtttgcgcc cattcctgtt cggccttgga tgccgtcatg
     2581 gcctcagggc cgcgccgata gaccatttga acgtcctgcg caccgagcag tttggcctgc
     2641 accgccgcat caatcgccgt catgccgccg cccaccacca ccacctggcg accaatcggc
     2701 agcttggaca agtcttcggc ctgacgcagg atggcgatga aatcaactgc gtcctgcacg
     2761 ccgtaaagcg cctcgccggg aatgccaagc tggtgcgtgt tgggcaggcc catgcccaga
     2821 aacagggcat cgaaatcctg ccgcagtgcc tcaagctggg ccacggtttg caacttccag
     2881 ccgtggcgca actcgatgcc gcccagaccc agcagccact gcacttcggc ctgggcaaag
     2941 ttattggggg tcttgtagct ggccaggccg tattcattga gaccaccggc ctttggcttg
     3001 gcatcaaaga tcaccacctg gtggccgagt ctggccagcg tgtgggcgca ggccagacca
     3061 gccgggccgg caccgaccac cgccaccttt ttgccggtgg ccgcggcacg ggtaaagatt
     3121 tgcggctgcg tgctttccat cagcgcatcg acggcgtggc gttgcagccg accaatggcc
     3181 accggtttgc cctcctgggt gttgcgcacg cacacgactt cacacaggtt ttcggtcgga
     3241 caaacccggg cgcacatgcc gcccaggggg ttggcctgca aaatggccgc agccgaaccg
     3301 cgcagattgc cgtcagcgat gcggcggata aacgagggca catcaatgtg ggtcgggcag
     3361 gcggtggcgc acggcgcatc aaagcaatac aggcagcgct cggcttcgag caccgcttgc
     3421 gggccggtca ggcgcggtgt ggcatcggcg aagcgccggg catattcggc agcgtcgagc
     3481 cggccggggc gtatatcggg cgcaatggaa agggacacgc tggcaactcc tgttgaatct
     3541 gacagattgg tcaagtttga ccacggatat tagcaagcca tgtgccaaac cgtgcgcagc
     3601 ggtggcacgc aagcgcattt tgtatggacg gcaaagcaaa atgcgtccac acctctgatt
     3661 caaagaaagc gagttttgca tgactgtcac ccactcgtcc ggtagctgcc tttgtggtgc
     3721 ggtggctttc accgctgact ggccgtcgaa atgggttgcg cactgccatt gcacgcgctg
     3781 tcagagtgcg cacggtgcgg cctttgtaat ttggatcggc agggtgggtc aatattcaat
     3841 cggcgcgaac aacgcaaagt cacaggtgaa tggaacctga tttgcctggc ctggaatgtc
     3901 aagcgcatgg gcgcatggca gtactgcgtc caaaagttgg ataaaggcag aaagcgcggt
     3961 gaaaacactc aaatcctcgt caaaccaaga ctgaattgcc tatgttttaa gcattttcag
     4021 gggtggcaca ttttgaaatc ctgtgcccca caggcgtgcg ccgggagacc ggcagggagt
     4081 aaagggtgaa agtcccaaac gatgaagaaa tagcgaatca cattgtcccc gagtcatgcg
     4141 cggcgtaccg tgaggtgcgg cgtgaagcgt tgacaggggt acacataggc cagaatttga
     4201 gccgcgaaat ggacattacc ccgagtgccg acgccgtgcc tgtggcgcaa ggctacacgg
     4261 ctgtgcgcga taaacgcgaa tccccctgtg caaccaacag cagtcaagca acaaacggcg
     4321 gccagcaaac ttgcacagaa aatgtgcagc ggatgatgca acgcccaagc cgccgactgg
     4381 ggagcaagtc tatctggcgt atgtgatgaa gcaaaagatg cagaagcaag cagacttgac
     4441 ttacgcgcaa cgcttgcgac agcaactggc aagcgctcaa tcgcaagttc gttaaacagg
     4501 gtcatcgctt catcgcagaa aaggcactta ccgcgcggtc gtgctgtctg cttgaactgt
     4561 tccccgtttg cgactcacca ctttataggc caagtcaata caggcattga agaacatgat
     4621 gacgatgaag cagcccaaaa aatcgcccac caccatcgcc gctgcgtggg cgaaaaaatc
     4681 gtcaagcttc accttgtctt gccaaacata gacgatgttg tgcacggtcc cattaaaaat
     4741 gctgcctgcg gctgacaaga tgatgatatg cagatagcgc aaattgctaa gcgcatcatc
     4801 taactggaca gtgcgtagtt ccccccttcg tgttcaggcc gccattggct g
//
LOCUS       DAOSLL010000356         4849 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15579_length_4849_cov_3.987276, whole genome shotgun sequence.
ACCESSION   DAOSLL010000356 DAOSLL010000000
VERSION     DAOSLL010000356.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4849)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4849)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4849
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15579_length_4849_cov_3.987276"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1391)
                     /locus_tag="PLB25_14565"
     CDS             complement(<1..1391)
                     /locus_tag="PLB25_14565"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HOE42831.1"
                     /translation="MSLKLCIAQLNLVVGDLPGNSQKIIQAATAAYQDGARLVLTPEL
                     SICGYAAEDLFLRPAFIAACDDAVKALALALAHLKDLCLVVGHPTGGDSRTRSVAVPE
                     RFNTASVLREGLIVASYAKRELPNYQVFDERRYFTPGNEVCVFEAGEPGHSAKVGLLI
                     CEDAWFEAPARLTREAGAELLAVINASPFHIGKGNERELMMRERVLECGLPLVYAHLV
                     GGQDEVVFEGHSFALNADGRLAGRAPSFVENSLLVQTRRAQSAITLEALIAPERTPDA
                     DLWDALVLGVRDYIGKNGFPSVLLGLSGGIDSALVLAVAVDALGRDRVRALMMPSPYT
                     ADISWIDARDMAARLGVRYDELSIVPEFEAFKATLAREFTGLAEDATEENIQARIRGT
                     LLMALSNKFGSIVLTTGNKSEMATGYCTLYGDMAGGFAVIKDLAKTTVFQLARWRNAH
                     DPYGTGQQPIPER"
     gene            1478..2701
                     /locus_tag="PLB25_14570"
     CDS             1478..2701
                     /locus_tag="PLB25_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE42832.1"
                     /translation="MEQQTVLNADDYVIRVLDSPLDVDATAWNRLLFAQSQPTPFMRH
                     EFLSALHSSGCATADTGWAPRFFLLQRQGELQAACVIYLKSHSQGEFVFDWGWARAYA
                     QHGLAYHPKAVCAVPFIPVPGTRLLAISQPARQMLANVLLDWCAQQQLTGLHNLFASD
                     EDIAAFQQAGMLLRHTVQFHWTNTEPGYASFDNFLATLSQDKRKKIRQERRKVAEAGV
                     SFQVLQGSEIRTDEWNFFFRCYERTYLEHGNPPYFNRAFFARMADQMPEDWLLFIAHQ
                     QGQPIASSLVAINASCVRAGGQKDSTNPVRVAYGRYWGALRRVDCLHFEACYYQPLAW
                     CIANGYQRFEGGAQGEHKLARALLPVTTTSAHWLAHPQFFSAVNNFLTQEREGISQYV
                     DELERHSPFKATTTQ"
     gene            3283..4449
                     /locus_tag="PLB25_14575"
     CDS             3283..4449
                     /locus_tag="PLB25_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42833.1"
                     /translation="MKYDDAEIYMDCEVEVDDIGLLSGTHIGFYLAWAVKTGLLANRL
                     SADAEAVLQDPTHARALLFDRCDGKLMSEDMNERGNAFTQDYYDARYISDYVALFALN
                     TDDPVALFTVDNTAANYAKVAQWLDARFGEWQNQAALPSDVKLLQLMEAQFAPWLLSQ
                     GFIRDPNAFYKKKARYIKTGPWGQHCLMLSALDDRPKMYGMALEVSSTFNTLAKWVSD
                     EMRIDNPRITSEPPATYYQPRHHWLGDWPVPLIPFRGRPVHAIPLTDVRQIEPAIGIL
                     CRRAQKVLPGLLASLETLEGYDQLYCGDPARVPEPWRVKTYNEGPFVSVSYLTLLRLL
                     CAELAHHPNLRAMCDDAERALAAAEPALRKQMFGAEVQEMRARLMRLRARMLSR"
BASE COUNT         1030 a         1434 c         1379 g         1006 t
ORIGIN      
        1 atgcgctcgg ggatgggttg ctggccggtg ccatacggat cgtgtgcgtt gcgccagcgc
       61 gccaactgaa acacggtcgt cttggccagg tccttgatga ccgcaaaccc gcccgccatg
      121 tcgccataca gggtgcagta gccggtggcc atctccgatt tgttgccagt tgtcagcaca
      181 atgctgccaa acttgttgct cagggccatc agcagcgtgc cgcgaatgcg agcctggatg
      241 ttttcttctg tggcatcttc ggccagaccg gtgaactctc gggctaacgt ggccttaaag
      301 gcttcgaatt cgggcacgat tgacagttcg tcgtagcgca cgcccaatct ggccgccatg
      361 tcgcgcgcgt caatccaact gatgtcggcg gtatagggcg acggcatcat cagcgcacgc
      421 acccggtccc ggcccagtgc atcgactgcg accgccagca ccagcgccga gtcgatgccg
      481 cctgacagtc ccagcaacac cgacggaaag ccgtttttgc caatgtagtc tcgcaccccg
      541 agcaccaagg catcccacag gtcagcatcc ggggtgcgct caggggcgat caatgcttcc
      601 aaagttatag ctgactgcgc gcgtctggtc tgcaccagaa ggctgttttc tacaaaactc
      661 ggggcgcgcc cggcgagcct gccatcagcg ttgagcgcaa atgaatgacc ctcgaacacc
      721 acctcatcct gcccgccaac caggtgcgca tagaccagtg gcaagccgca ttccagcacc
      781 cgctcacgca tcatcagttc acgctcgttg cccttgccaa tgtgaaacgg cgatgcgttg
      841 atcaccgcca acagttcggc accggcctcg cgggtcagcc gcgcgggcgc ttcaaaccag
      901 gcatcttcgc aaatcagcag accaactttt gcgctgtggc ccggctcgcc agcctcaaac
      961 acacacacct cgttgccggg tgtgaaatag cgtcgctcat caaacacctg gtagtttggc
     1021 agttcccgct tggcgtagct ggcaacaatc aggccttcac gcagcacgct ggcggtgtta
     1081 aagcgctcgg gtaccgccac cgagcgggtc cggctgtcac cacccgtggg gtgaccaacc
     1141 accagacaaa ggtctttcag gtgtgccagc gcgagcgcca gcgctttcac ggcatcatca
     1201 caggcggcaa taaaggccgg gcgcaagaac aggtcttcgg cggcgtaccc gcagatcgac
     1261 aattcagggg tcagtaccag ccgggcaccg tcctgatacg cggccgttgc cgcttgaatg
     1321 attttttgcg agttgccagg cagatcgccc accaccaggt tgagttgggc aatgcagagt
     1381 ttgagtgaca tgaaaggtac tgaaaatagg taacgaccaa gcgaccccgc aaggaccgca
     1441 cgccacgacg cttcaagctg atttgtcagg aaagataatg gaacaacaga ccgttttgaa
     1501 cgcagacgat tatgtcattc gggtgcttga ttctccgctc gatgtggatg ccacagcctg
     1561 gaaccggctg ttgtttgccc aaagccagcc aacccccttc atgcgccatg agttcctcag
     1621 tgcgctgcac agcagcggct gcgccaccgc cgacactggc tgggcaccca gattttttct
     1681 gctgcagcgc cagggcgagt tgcaagccgc gtgtgtgatc tacctcaagt cacactcgca
     1741 aggtgaattt gtttttgact ggggctgggc gcgcgcctac gctcagcatg ggctggctta
     1801 ccaccccaaa gcggtgtgcg ccgtgccctt cattccagta ccgggcaccc ggctgctggc
     1861 catatcacag ccagcgcgcc agatgctcgc caatgtgttg cttgactggt gcgcgcagca
     1921 gcagctcacc ggtctgcaca acctgtttgc cagtgacgaa gacatcgccg cctttcaaca
     1981 ggccggcatg ctgctgcgcc ataccgtaca gttccattgg accaataccg agcccggcta
     2041 tgccagtttt gataactttc tggccaccct gtcacaagac aagcgcaaga aaatccgcca
     2101 ggagcggcgc aaggtggccg aagccggcgt gagctttcag gtgctgcaag gcagcgagat
     2161 ccgcacagat gagtggaatt tcttctttcg ctgttacgaa cgcacttacc tggagcatgg
     2221 caacccgccc tacttcaacc gcgccttctt tgcccggatg gccgaccaga tgccggagga
     2281 ctggctgctg tttatcgccc atcaacaggg ccagcccatt gcttcaagtt tggtagctat
     2341 caacgcaagc tgcgtaaggg ctggaggtca aaaagattca acaaatccgg tcagggtagc
     2401 ctatggccgc tattggggcg cgctaaggcg ggttgactgc ctgcactttg aagcctgtta
     2461 ttaccaacca ttggcctggt gcatcgccaa cggttatcag cgctttgaag gtggcgcgca
     2521 gggcgaacac aaactggcgc gcgccctgtt gccggtcacc accaccagcg cccactggct
     2581 ggcgcatcca cagttttttt ctgcggtgaa caactttttg acgcaagagc gtgaagggat
     2641 cagccagtac gtggatgaac tggagcggca tagtccgttc aaggcgacga ccacccaatg
     2701 aagtttcgcg gcttgggcga ctgtgggtgc cgcgcagcag ccggacaggg cggcccctca
     2761 tgctggcgta ccagaaatcg gggccgacct gtccggctgc tacgccgagg ccctggagtt
     2821 tgcgcacggg cagtttgaat accgcaagct gcacgtcaac gatgcgttca acggatggcg
     2881 atggcaggaa tgatgctgag tgcctggcat ttacaggtga aattggtctg tagccctcgt
     2941 tgtatatgcg ctgctagctc tcttttttat agctatatcg ttttgttttt tgtatgaggg
     3001 atgacgggct tcttgagacc cattgcggcc attcaacaaa attgccaagt gatcaccgga
     3061 aagtcgacag cagccgttcg atttcgctgg aagcaaactt ttgtttttga catccgaatc
     3121 tcggtaccgg tcgttcagcg gtaaagaaca ccttttcaaa ctggtggtcg gcactgcagc
     3181 gtttgcacac ggccatgtct agctgagagc agtgccgctc tgatcaagcg cgtaggttgt
     3241 cggcaaaacg tggctatttg aactcccaaa agttgatctc ccatgaaata cgacgacgct
     3301 gaaatctaca tggactgcga ggtcgaggtt gacgacatcg gcctcctgtc tggcacccac
     3361 attggttttt acctggcctg ggcggtcaaa accggcttgc ttgcaaacag gctcagtgct
     3421 gacgcagaag ccgttttgca agaccccacc cacgcacgcg ccctgctgtt tgaccgctgt
     3481 gacggcaagc taatgtcaga agacatgaac gaacgtggca acgccttcac gcaggactac
     3541 tacgacgcac gctacatcag cgactacgtg gccctctttg ccctgaatac ggacgacccg
     3601 gtggcgcttt tcacggttga caacactgcg gccaattacg ccaaagtcgc gcaatggctc
     3661 gatgcccgct tcggggaatg gcaaaaccag gcggctttgc ccagcgatgt caagttattg
     3721 cagttgatgg aagcgcagtt cgcgccttgg ctgctgtcgc agggttttat acgcgaccca
     3781 aacgcgtttt acaagaaaaa agcccgttac atcaagaccg ggccatgggg ccagcactgc
     3841 ctgatgctgt cagcgttgga tgaccgcccc aaaatgtatg gcatggcgtt agaggtgtcc
     3901 agcaccttca acacgcttgc gaaatgggtg agcgatgaaa tgcgcatcga caacccgcgc
     3961 atcaccagcg aacccccagc cacttattac cagcccaggc atcattggct aggcgactgg
     4021 ccggtgcccc tgattccctt tcgcggcagg ccggttcacg ccatcccgtt gaccgatgtc
     4081 cgccaaattg agcccgccat tggcatcctg tgccgtcgcg cccagaaagt gctgccaggt
     4141 ctgcttgcat cgcttgaaac cctggaggga tatgaccagt tgtattgcgg cgacccggcg
     4201 cgcgtgcctg agccgtggcg ggttaaaacc tacaacgagg ggccatttgt gagcgtgagc
     4261 tacctgacgc tgctgcgact gctgtgtgcc gaactggcgc accaccccaa tctgcgggcg
     4321 atgtgtgacg atgccgaacg ggccttggct gctgccgagc ccgccttgag aaaacagatg
     4381 ttcggggcag aagtgcagga aatgcgcgca cgactgatgc ggttgcgcgc caggatgctg
     4441 agtcgatgaa tcattggtga ccttaggact ggaatcgctg cagcgccgac cgccacagac
     4501 aaaaaagatt gtcacccccg aacgaccgct tcggagtttt agcggcgaag aaagcaagat
     4561 attggaaggg caccggcaat gaccgcgttg gagaaatcgc ctcaaattct ttgtgcgcgg
     4621 tgagcgacca gtaacgctgc agtgccgacc gccagtttga aaaggtgatt ttcaccgctg
     4681 aatgatcgct cctgagattc gggtgtcaaa aacgaaagtc tgcttccggc gaaattgaag
     4741 gtcagctttt gacgtttctt tgggcttcat gctgacccgg tgtacgtcgg cttcgggtct
     4801 tctgaagtcg gtcatcccaa ccgagcacaa cgggtatctt catgtctct
//
LOCUS       DAOSLL010000357         4840 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15613_length_4840_cov_3.735841, whole genome shotgun sequence.
ACCESSION   DAOSLL010000357 DAOSLL010000000
VERSION     DAOSLL010000357.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4840
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15613_length_4840_cov_3.735841"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="PLB25_14580"
     CDS             complement(<1..705)
                     /locus_tag="PLB25_14580"
                     /inference="COORDINATES: protein motif:HMM:NF013109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HOE42834.1"
                     /translation="MTEDKPATFAAPTGPAAPAPRVVKNPLAGDIWGGMAAMLVALPS
                     AIAFGVTIFAPLGGTLAAQGALAGILGATALGLVAAAFGGCQRLISAPCAPAAAVLSA
                     LAISFTQQGIAASAVVLMLGLIGLLSGAFQIGLGLIGVGRLINYIPFPVVSGYLSGVG
                     LIIIGSQIPKWLGAPAGTDWIKALLSPSLWTWQSGVVGAVVMATMLLTPRLTTAVPAA
                     ILALLAGIGSYLLLGLA"
     gene            950..1801
                     /locus_tag="PLB25_14585"
     CDS             950..1801
                     /locus_tag="PLB25_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein"
                     /protein_id="HOE42835.1"
                     /translation="MDTTKPSTFSTSVTFDTACTPAGLASQLAAGAAPLIIDVRQNEA
                     FAASDSTLPGALRRDPHQVASWAATLPAAAAVLVYCVHGHEVSQGVWRALRQRGIHAT
                     FLQGGLQAWREAALGMAIKPAGSATRWITRERPKIDRVACPWLVRRFIDAQAQFLYLP
                     TAQVSAVAAAMGAMPYDVNASVAETLFTHDGDACSFDAFIQHYHLRADTALAKLADIV
                     RGADTDRLDLAPQAAGLLAVSLGMSRMMTDDLAMLEAMMPFYDALYAWCRDAVAGVDE
                     PHNWKPA"
     gene            1798..3213
                     /gene="chrA"
                     /locus_tag="PLB25_14590"
     CDS             1798..3213
                     /gene="chrA"
                     /locus_tag="PLB25_14590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HOE42836.1"
                     /translation="MSADLSRGNALPGTAPAQPPAPVVSFFEALKFWLKLGFISFGGP
                     AGQIAIMHRELVEEKRWISEGRFLHALNYCMLLPGPEAQQLATYIGWLMHRTLGGVVA
                     GILFVLPSLFILVGLSWVYVAFGNLPWVAGLLYGIKPAVAAIVLQAVHRIGAKTLQKP
                     AAAPVLWAVAVISFLAIGVLKIAFPWVVLGAALTGWLGQRWAPGQFAPVGGHGSTTAA
                     GRAHPPALIDDGTPTPAHARFSKSRLAGVLATGLTLWLLPMGLLMAWQGWDGDLTQMG
                     WFFTKAALLTFGGAYAVLPYVYQGAVEQFGWLTAAQMMDGLALGETTPGPLIMVVAFV
                     GFLGGWVKQVLGSETLFLGAALAATVVTWFTFLPSFLFILAGGPLVESTHGKLQFTAP
                     LKAITAAVVGVIASLALFFIAHIAEGTGAGGVFGLKVDVMALVLTALAALTLLRYKVG
                     VVNVIAASALAGLVLRLAGWA"
     gene            3353..4177
                     /locus_tag="PLB25_14595"
     CDS             3353..4177
                     /locus_tag="PLB25_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HOE42837.1"
                     /translation="MPRFAANLTLLYPELPFLDRFEAAARDGFSAVEYLFPYAWPKSE
                     LLARLRGNGLQQVLFNAPPGGVDGPTSVQAWDNGCRGLAAVPGREAEFRQGMALALDY
                     CAALACPRLHVMAGLLPSGLARDSLLPVIAANLRWACEQAAMLGVTLLIEPINLRDMP
                     GFYLNRQEQAHHILDAVGAPNLQVQMDLYHCQITEGDIATKLRRYLPTGRVGHIQVAG
                     VPERHEPDVGELNYPYLFSLIDTLGYSGWVGCEYRPRLAAQVNGTSAGLSWRAPWL"
     gene            complement(4492..4620)
                     /locus_tag="PLB25_14600"
     CDS             complement(4492..4620)
                     /locus_tag="PLB25_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42838.1"
                     /translation="MDSALPAIYSEAFRVFGKTPANAETGFPTAADVAATRLPWRA"
     gene            complement(4634..>4840)
                     /locus_tag="PLB25_14605"
     CDS             complement(4634..>4840)
                     /locus_tag="PLB25_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020067677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE42839.1"
                     /translation="AGHANAHGAGDAALLPRGGGVLQGSITSTVSIGVASTTASENRD
                     FDYLYSHADKALYNAKEKGRNRVI"
BASE COUNT          791 a         1503 c         1554 g          992 t
ORIGIN      
        1 ggccagcccc agcagcaagt agctgccaat gcccgccagc aaggccaaga tggcggccgg
       61 cactgccgtg gtcagtcgcg gtgtcagcag catggtggcc atgacaaccg cgccgaccac
      121 cccgctttgc caggtccaca gcgagggcga cagcagggcc ttgatccagt cggtgccagc
      181 cggggcaccc agccacttgg gaatctggct gccgatgatg atcaggccga caccgctgag
      241 gtaaccgctg accaccggaa acgggatgta attgatcagc cgcccgaccc cgatcaggcc
      301 cagcccaatc tgaaaagccc ccgacagcaa accaatcagg cccagcatca acaccaccgc
      361 agaagccgca atgccctgct gggtaaagct gatggccagc gccgacagca ccgcagccgc
      421 tggtgcgcag ggtgcactga tgaggcgctg gcaaccgcca aaagcagcgg caacgagccc
      481 gagtgcggtc gcccccagaa tgcccgccag cgcgccctgt gccgccagtg tgccccccag
      541 cggcgcaaaa atggtgacac caaaagcaat ggctgacggc aacgccacca gcatcgcggc
      601 catgccgccc cagatatccc cggccaacgg gtttttgacc accctgggcg cgggtgccgc
      661 aggtccagtt ggcgctgcga atgttgcagg cttgtcttca gtcatggatg caggttgggt
      721 gaggtgggcc aatgctggtg cttgcctgtg tcatctgaca catttgcgtc acattgtggc
      781 cccaattggg cgcagcggat gatgccgcca caacgtcttg atgttggtgt cgggcgccgc
      841 cgggctgtgc taaagtcatg cggtcggggt gtcagctccc cggcatcggg ctcaaccaag
      901 ccccaagacg gtgtcccgtc caatcgctgc aaccttgatc ggacttttca tggacaccac
      961 aaaaccatcc actttttcga cttccgtcac cttcgatacc gcctgcaccc ctgctggtct
     1021 ggcctcgcag ttggcagcag gtgctgcgcc actgatcatc gacgtgcgcc agaacgaggc
     1081 ctttgccgct tcggacagca ccttgcctgg tgcattgcgg cgtgatccgc atcaggtggc
     1141 aagctgggct gccaccttgc ctgcagcggc ggcggtgctg gtgtattgcg ttcacgggca
     1201 cgaagtcagt cagggcgttt ggcgtgcgct gcgtcaacgt ggcattcacg cgactttttt
     1261 gcaaggcggc ttgcaggcct ggcgcgaagc cgctctgggc atggcgatca agccagccgg
     1321 tagtgccacg cgctggatta cgcgggaacg ccccaagatt gaccgcgtcg cctgtccctg
     1381 gctggtgcgc cgctttatcg acgcgcaggc gcagtttctc tacctgccca cggcgcaagt
     1441 cagcgctgtc gcggctgcca tgggtgccat gccgtatgac gtgaacgcca gcgttgccga
     1501 aacattgttt acccacgacg gcgatgcgtg cagctttgat gcgttcatcc aacactatca
     1561 cctcagggcc gacaccgcgc tggccaaatt ggccgacatc gtgcgcggtg ccgataccga
     1621 ccggctcgac ctggccccgc aagccgctgg cctgctggcc gtgtcgcttg gcatgtcgcg
     1681 catgatgacg gacgatctgg cgatgctcga agccatgatg ccgttttacg atgcccttta
     1741 cgcctggtgc cgcgatgcgg tggccggtgt ggacgagccg cacaactgga aaccggcatg
     1801 agcgcggacc tgtcgagagg caacgcgttg cctggcacgg cgccagccca gccgcctgcg
     1861 ccggtcgtgt cgttttttga ggcactcaag ttctggctga aactcggctt catcagcttt
     1921 ggcggcccgg ccgggcaaat tgccatcatg caccgcgaac tggtggaaga aaaacgctgg
     1981 atctccgaag gccgcttctt gcatgcgctg aactactgca tgctgttgcc cggccccgag
     2041 gcgcagcaac tggccaccta catcggctgg ctgatgcacc gcactttggg tggcgtggtc
     2101 gctggcatct tgtttgtgct gccgtcgctg ttcattttgg tgggcttgag ctgggtttac
     2161 gtggcgtttg gcaatctgcc ttgggtggcc gggctgctgt atggcatcaa gccggcggtt
     2221 gcggcgattg tcttgcaggc ggtccatcgc atcggcgcaa aaacattgca aaaaccggcc
     2281 gcagcgcccg tcctgtgggc tgtggcagtt atcagtttcc tagctattgg ggtgctgaaa
     2341 attgcttttc catgggttgt gctgggtgcc gccctgaccg gctggctggg tcaacgctgg
     2401 gcacctgggc aatttgcgcc agttggcggg catggctcaa ccacggcagc ggggcgcgcg
     2461 cacccgcccg cgctgattga cgacggcacg cccacgccag cccatgcccg gttcagcaag
     2521 tcccgcctgg caggggtgct tgccacaggc ctgacgctct ggctgttgcc gatgggtctg
     2581 ctgatggcct ggcagggctg ggacggtgac ttgacgcaaa tgggatggtt ctttaccaag
     2641 gcagcattgc tgacctttgg tggtgcctac gcggtgttgc cttacgtcta tcagggcgcc
     2701 gtcgagcaat ttggctggct caccgccgcg cagatgatgg acgggctggc gctgggtgag
     2761 accacgcccg gcccgttgat catggtggtg gcgtttgtcg gttttctggg tggctgggtc
     2821 aagcaggtgc tggggtcgga gacgctgttt ctgggggctg ctctggccgc cacggtggtc
     2881 acctggttca cctttttgcc ctcgtttctc ttcattctgg ccggtggccc gctggtggaa
     2941 tcgacccacg gcaagctgca gttcaccgcg ccgctcaagg ccatcaccgc tgcggtggtc
     3001 ggtgtgattg ccagtcttgc tttgtttttt atagctcaca tcgcagaagg tacgggggct
     3061 ggaggtgttt ttggcttgaa agtcgatgta atggcgctgg tactgaccgc gctggcggcg
     3121 ctgaccttgc tgcgctacaa agtgggcgtg gtcaatgtca ttgccgccag cgccctggcc
     3181 gggctggttt tgcggctggc cggctgggct tgaccgcatg ccgggcgctg gcaacccgat
     3241 gtcgggccct gtcatcagac acaatgccga atgcccgaat ccccgagaac ccggccagaa
     3301 ctttcatttg ctttcgttta gatagctgtc tgcgcatact ggacccatcc ccatgccacg
     3361 ctttgccgcc aacctgaccc tgctttaccc cgagttgccg tttctcgacc gctttgaggc
     3421 cgctgcccgc gacggtttta gcgcggtcga atacctgttc ccctatgcct ggcccaagtc
     3481 tgaactgctg gcgcgcctgc gtggcaatgg tttgcagcag gtgctgttca acgcgccacc
     3541 cggcggtgtc gatggcccca ccagcgtgca ggcctgggac aacggctgcc gcggtttggc
     3601 ggctgtgcct gggcgcgagg ctgaattcag gcagggcatg gcgctggcgc tggactactg
     3661 cgctgcactg gcctgcccgc gtttgcatgt gatggcgggc ttgctgccaa gcggcctggc
     3721 gcgcgacagc ctgctgccgg tcatcgccgc caacctgcgc tgggcctgtg agcaggccgc
     3781 catgctgggg gtgacgctgc tgatcgagcc gatcaacctg cgcgacatgc cgggctttta
     3841 cctcaaccgg caagagcagg cgcaccacat tctggacgca gtgggcgcgc ccaatctgca
     3901 ggtgcagatg gacctgtacc actgccagat caccgagggc gacatagcca ccaagctgcg
     3961 ccgctacctg ccaaccggac gcgtcgggca catccaggtc gccggggtgc cagaacggca
     4021 cgagcccgat gttggcgagc tgaactaccc gtacctattc agtttgatcg acacgctggg
     4081 ttacagcggc tgggtcggct gcgaataccg gccgcgtctg gcggcgcagg tcaacggcac
     4141 ctctgccgga ctgagctgga gagcgccctg gctgtagccg cgttgggtgt ggtgcgctcg
     4201 gaaagggcgt tccagtgccg tggcgtgaat cctggattcc tcaatcgacc gcttgttttc
     4261 ctcggtaagt ctcaatgagc agttactttt tcagctttga cggggtttgc cctttgggtc
     4321 gcagcgcggc gtacatgatt gagccactgg caacacgcct gggggcgttg cgccgtaatc
     4381 gtccgccagg gcatgctgca tcctggttga accgcctgtg cttgcgttgc ggaaatcaga
     4441 tgcgcggccc tggcagtcgt catccttggc tttgacttga tggctggcgg atcaggcccg
     4501 ccacggcagg cgcgtggcgg ctacgtcggc cgccgttgga aacccggttt cggcgttggc
     4561 cggggttttg ccgaacaccc ggaatgcttc tgaataaata gctggcagcg cactatccat
     4621 aagggctaga gccctaaatg acccgatttc tgcctttttc cttggcgttg taaagtgcct
     4681 tgtcagcatg gctgtagagg tagtcgaaat cgcggttttc gctggctgtg gtgctggcca
     4741 cgccaatgct cacggtgctg gtgatgctgc cttgcagcac acccccaccc cgcggcagca
     4801 aggcagcatc accagcaccg tgagcattgg cgtggccagc
//
LOCUS       DAOSLL010000358         4835 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15627_length_4835_cov_5.163808, whole genome shotgun sequence.
ACCESSION   DAOSLL010000358 DAOSLL010000000
VERSION     DAOSLL010000358.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15627_length_4835_cov_5.163808"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /locus_tag="PLB25_14610"
     CDS             complement(<1..1044)
                     /locus_tag="PLB25_14610"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE42840.1"
                     /translation="MLSDNFSSLFDLLPIGAYRSSPSGKQLRANPALVALNGYSTEAE
                     MLACVRDIATEWYCEATRRDQFTSQLHQQGRVVDFVSEIYRHKTRERIWVREHAHQVF
                     DHHGRVAYFEGTVEDITREHQARLDLQASESRFRAMTELSSDWYWEFDANMRFTRLDV
                     GRRSVSVGVSQEVLGKTRQQLSHIEMSPEQWKHYQALLDERQVFHDFEFPSRTLAGHL
                     IWYAISGEPMFDAAGDFAGFRGIGRDVTVRKQSEELVRQMAFHDALTGLANRRLFMDR
                     LHQALLSMQRSGQVGVLMFLDLDKFKALNDLMGHGAGDVLLQQVAQRIKGCVRGIDTV
                     ARLGGDEFTVLLEN"
     gene            2089..>2969
                     /locus_tag="PLB25_14615"
     CDS             2089..>2969
                     /locus_tag="PLB25_14615"
                     /inference="COORDINATES: protein motif:HMM:NF025596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4338 domain-containing protein"
                     /protein_id="HOE42841.1"
                     /translation="MICEHPQGAGPLVGCQLRFLINSDHGWLGGFSFSAAALKLRDID
                     QWIGWNTDQHRAHLHRVLGMSRFLLRTSMHCHNLASCVLGMVLRQVGPDFEAQYGYRA
                     WLVESFVDTEHFLGTCYKATNWIDIGQTQGRGRQDREHKVAKSVKTIYVYVIEPEWRT
                     KMGVAQPMGVQPLEIGQGLDADQWAAQEFGGANLGDSRLNERLVSSAQALGAMPGRTL
                     SGARQGDWPAVKGYYRMIDKPDDSALNVQAILAPHRERTVQRMMGQSTVLCIQDGTDL
                     NYNQLDQCIGLGVLSKN"
     assembly_gap    2970..3069
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3070..3474
                     /locus_tag="PLB25_14620"
     CDS             <3070..3474
                     /locus_tag="PLB25_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42842.1"
                     /translation="READFFELFDEQRKTAKVDLLVRSKHDRVVDDDVKAVHLFESVR
                     QSPICGEMVIEVQRQSTRTKKSKQQAKPGHVQRSATVILRYKEIELRPGAHQKDKAPI
                     KLTVVHVQETTQPKDDEPVEWSFADDLRCIKP"
     gene            3422..3808
                     /locus_tag="PLB25_14625"
     CDS             3422..3808
                     /locus_tag="PLB25_14625"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOE42843.1"
                     /translation="MNRLSGLLLTTCDVSSPEQAQQILRWYCLRWRIEDWHRVLKSGC
                     NIEKLQHKTAERLKRTIAINLVVAWRIMLLTLLGRECPQLAAEVLSSDLEIQVLIANS
                     KKTNSTTLAGSSRAHGRSTRWLPWAR"
     gene            complement(3931..4704)
                     /gene="rlmB"
                     /locus_tag="PLB25_14630"
     CDS             complement(3931..4704)
                     /gene="rlmB"
                     /locus_tag="PLB25_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HOE42844.1"
                     /translation="MSNPKVLFGFHAVGVRLKTAPKSVIEVYFDPTRRDSRMRQFVEK
                     VNESGVRLIEADGIRLAKMSGSHGHQGVAARVHELTQVKSLDELLEQLEAGQASKLPA
                     EREQPLLLVLDGVTDPHNLGACLRVADGAGVHAVIAPKDHAAGINATVAKVASGAAET
                     VPYFMVTNLARTLNELKERNIWIVGTSDGADKTLYQADLRGPVALVLGSEGDGMRQLT
                     AKTCDELVSIPMRGAVESLNVSVASGVCLYEALRQRSAR"
BASE COUNT         1066 a         1367 c         1316 g          986 t
ORIGIN      
        1 attttccaga agcaccgtga actcgtcgcc acccaggcgc gccacggtgt cgatgcccct
       61 gacgcagccc ttgatccgct gtgccacctg ctgcagcaac acatcaccgg caccatgccc
      121 catcaaatcg ttgagtgcct tgaatttgtc gagatcaaga aacatcaata caccaacctg
      181 cccactgcgt tgcatgctca acagtgcctg atgcaggcga tccatgaaca agcggcggtt
      241 ggccaacccg gtcagggcat catgaaacgc catttgacga accagttcct cagactgctt
      301 tcgcactgtc acgtcgcggc caatgccccg aaacccggca aaatcgccag ccgcatcaaa
      361 catcggctca ccactgatgg cgtaccagat cagatgacct gccagggtac ggctcgggaa
      421 ctcaaaatcg tgaaaaacct gcctttcatc cagcagtgcc tggtagtgct tccattgctc
      481 aggactcatc tcgatatggg ataactgctg gcgtgttttc cccagcactt cctggctgac
      541 accgacactg acactgcgtc gcccgacatc gagtcgggta aagcgcatgt tggcatcaaa
      601 ctcccagtac cagtccgacg acaattcggt catggcacga aagcggcttt cactggcctg
      661 cagatccagc cttgcctggt gctcccgggt aatgtcttcg actgtgcctt caaagtacgc
      721 cacccgaccg tgatgatcaa acacctggtg agcatgctca cgcacccaaa tccgctcgcg
      781 tgtcttgtgc cggtaaatct ccgagacaaa gtccaccacc ctgccctgct gatgaagttg
      841 cgaggtaaat tgatcgcgcc gggtggcctc gcaataccac tcggtcgcga tgtcccgcac
      901 gcaagccagc atctcagctt cggtgctgta gccattgagc gcaaccagcg ccggattggc
      961 acgcaactgc ttgcctgaag gactcgatcg gtaagcacca atcggcaaca ggtcaaaaag
     1021 gctggagaag ttgtcactga gcaatgcagt ccttccaaag gataggtcgc gtgaaaaatc
     1081 tgattttcca ctgaaatggt gccaggttct tgacctgccc gggttgtcaa gacttcttgg
     1141 agcctcaaca gaggtaagaa ggttgattcg ttttgtggcc ggtgacctgc accgcttcat
     1201 ggatttggcg aagaaagagc tggcaggacg tggacggcat gaactaacgg gctgttggat
     1261 tgtctctgtc tagactcatc agaccaccgg gaacccgtca attgttccga caagcgctcc
     1321 agcgctgacc agggaactcc cggtagcagc tacctggaaa aagtacaacg ggcgcgcagg
     1381 agatcgggtg cgctgcgacc cgccaccagg tcacacagcg atgcatcagg atgccgcgcc
     1441 agaaatatca aaaaccatag ctgccagcgc ttgtagggcg ggggctggag gccaatttgg
     1501 cgtataaaaa tttcaagcag gtcgggttgg cgcagcgtaa cccgacatcc gccagcacgc
     1561 ctgtttgtcg gtttacgcgc ttcgcacttc acgctaaccc gacctgcggg gaagtcaaaa
     1621 tcaatagctg ccagcgcttg ccagacgggg gctggagctc cccagtaccc caaagtaaag
     1681 ctgaactttt tttggcatct tgtttactct gcacagatgc agcaacaaaa ccagatcaag
     1741 cgaacccttg cacaacccga atcgctggac atcgttcgca gtcatctcgc cagcaatact
     1801 cataaaaacc gcacgtcgct gtccaaggcg ctgtgtcagc acttcaactt ctctgatgca
     1861 cgcgcaggcc tgcaaatcgg aggctgcaac aaagcgttac gggaattgga gcgtgccggt
     1921 cactttattt tgcctgtctc tggcaggctt aagtccgtca aaaacccacg tcgcctgggg
     1981 cttgccgtgc ccgacccagt tgatgtgccc tcgcttgtag gcgctgtaca ggggctcgta
     2041 ctggtgaagg tcgacagtgt tgatttgatg cgaatctgga atgagatgat gatctgtgag
     2101 cacccgcaag gtgctggccc cctggtgggg tgccaactgc gcttcctcat caattccgat
     2161 catggctggc tcggtggttt ttccttcagt gctgcggccc tgaagctgcg tgacatagat
     2221 caatggatcg gctggaacac cgatcaacac cgtgcgcacc tgcaccgcgt gctaggcatg
     2281 agccgctttc tgctgcgcac cagtatgcat tgccacaacc tggcctcttg tgttcttggc
     2341 atggtattgc gacaggtggg gccggacttt gaggcccagt acggctatcg agcctggctg
     2401 gtggagagtt ttgtcgatac cgaacacttt ctgggcacgt gctacaaggc gaccaactgg
     2461 atcgacattg ggcaaaccca gggacgtggg cgtcaggacc gcgaacacaa ggtagccaaa
     2521 tctgtcaaaa ccatctacgt ctacgtgatt gagcccgagt ggcgtaccaa gatgggggtg
     2581 gctcaaccga tgggcgtgca gccattggaa atcggtcaag ggctcgatgc agatcaatgg
     2641 gccgcgcaag agtttggcgg tgccaatctg ggtgacagcc gtctcaatga gcgcctcgta
     2701 tcaagcgccc aagccctggg agccatgccc gggcgcacct tgagcggtgc ccgtcagggt
     2761 gactggccag ccgtcaaggg ctactaccgc atgatcgaca agcccgacga ctcggccctt
     2821 aacgtgcaag ccattttggc gccacaccga gagcgaaccg tgcaacgcat gatgggtcaa
     2881 agcactgtgc tgtgcatcca ggacggcact gacctgaact acaaccagct cgatcagtgt
     2941 atcggtctgg gcgtgttgag caaaaaccan nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3061 nnnnnnnnnc gcgaagcgga cttctttgag ctcttcgatg agcagcgtaa aacggctaag
     3121 gttgatttgc tggtgcgctc caagcatgac cgcgtcgttg atgatgatgt taaggctgtg
     3181 catctctttg agtcagtcag gcaaagtccg atttgtggcg agatggtgat tgaggtgcaa
     3241 cggcaaagca cacgcaccaa gaagagcaaa cagcaggcca aacccgggca tgtgcagcgt
     3301 agcgccacgg tgattttgcg ttacaaggaa attgaacttc gccctggagc ccatcaaaaa
     3361 gacaaggccc cgatcaagct caccgtggtg catgttcagg agacgaccca gcccaaagat
     3421 gatgaaccgg ttgagtggtc ttttgctgac gacctgcgat gtatcaagcc ctgagcaggc
     3481 gcagcagatt ttgcgttggt actgcttgcg ctggcgcatt gaggattggc atcgggtgct
     3541 caagagtggt tgcaatatcg agaagcttca gcacaaaact gccgagagac tcaaacgcac
     3601 gattgccatc aatttggtgg tcgcctggcg catcatgttg ctcactctgt tgggccggga
     3661 gtgcccgcaa ttggctgctg aagttttgtc ctcggatttg gaaatccagg tgctcatcgc
     3721 gaattcaaaa aaaactaact caaccactct tgctgggtca agccgtgcgc atggtcgctc
     3781 aactcggtgg ctaccttggg cgcgctaaag acagcccgcc tggccatcaa ctcatgtggc
     3841 aaggctacgc ctgtttgcaa atgatgtgcg ccggattcgc gcttgcttca gcaagctcag
     3901 gatgaaaaca gttgtcggta aagggtagtg ctagcgagcg ctgcgctggc gcaaggcttc
     3961 atacaaacac acgccactgg ccaccgacac gttcagactt tcgaccgcgc cacgcatcgg
     4021 aatgctgacc agctcgtcac aggttttggc ggtgagctgg cgcataccat cgccttccga
     4081 gcccagcacc agcgccactg gacccctcag gtcggcctgg tacagcgttt tgtcggcgcc
     4141 atcgctggtg ccgacgatcc agatgttgcg ctccttgagt tcattcaagg tgcgcgcgag
     4201 gttggtcacc atgaaatacg gcaccgtctc agccgcaccg ctggccactt tggcgacagt
     4261 ggcgttgatg cccgcggcgt gatcctttgg cgcaatcacc gcatgcacac cggcaccatc
     4321 ggccacgcgc aggcaggcac ccaggttgtg cgggtcagtc acaccgtcga gcaccagaag
     4381 cagcggctgc tcgcgctcag cgggtagctt gctggcttgc ccggcttcga gctgctccag
     4441 cagttcatcc agagacttga cctgggtcag ctcatgcacg cgcgccgcga caccctgatg
     4501 cccatggctg cccgacattt tggccaggcg aatgccgtcg gcctcaatca aacgcacccc
     4561 tgattcgttc actttctcaa caaattggcg catgcgcgag tcgcgtcggg tcgggtcgaa
     4621 atagacctca atgacggact tgggcgcggt tttcagccgc acgccaacgg catgaaaacc
     4681 gaacaagact tttggatttg acatgccttg attatcactg gcgaggttta gaccaattcc
     4741 agggcatctt gcagttcatt gcagaagcag tgtcatctca agatgaccct aaagagggta
     4801 aaactattca cgtgggatga agtagcaaac ccgct
//
LOCUS       DAOSLL010000359         4812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15716_length_4812_cov_8.654404, whole genome shotgun sequence.
ACCESSION   DAOSLL010000359 DAOSLL010000000
VERSION     DAOSLL010000359.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15716_length_4812_cov_8.654404"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            156..4812
                     /locus_tag="PLB25_14635"
                     /pseudo
     CDS             156..4812
                     /locus_tag="PLB25_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015740.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1039 a         1639 c         1363 g          771 t
ORIGIN      
        1 cagcgcacgc cagtctggct acccaagtgg caaaacctca cgccagcctt gccccagcgg
       61 gggcagcagc cgcagcatct ggggcagtac ttgcgcaatt ggccgcatca gccgcgcagc
      121 cgcttgctgt ggcgcagtcg gccaacgctg gcacggccca ggcgctcaag ctgctgtttg
      181 aagaaatctg cacccggctc aagtacctga gcgaagttgg cattggctac ctgaccctgg
      241 accgccaaag ccgcaccctg tccggtggcg aggtgcagcg catcaacctg accaccgcgc
      301 tgggcacctc gctggtcaac accctgtttg tgctggacga gcccagcatt ggcctgcacc
      361 cgcgcgacat gcaccgcatc atcgttgcca tgcagcgcct gcgcgacgct ggcaacaccc
      421 tggtggtggt ggagcacgac ccggccgtca tgctggcagc agaccgcatg atcgacatgg
      481 gccccggccc gggcgagcgc ggcgggcaga tcgtttttga tggcagcacc gccgacctgt
      541 gccgcgccga caccctgacc ggtgcctacc tgggcgggcg caagcaggtc ggtgtcggtt
      601 tcaagcggat ggtggccagc aacacgccgc gcctgatcct tgaaggcgcg cgcgaccaca
      661 acctgaaaaa cctgtcggtc gagatcccct tgcagcgcct ggtgtgcatc accggtgtca
      721 gtggttctgg caaatcgacg ctgatccagg acattctggc cccggccctg ctgcgccatt
      781 ttggcaaggc caccgatgcc ccaggcgcgt ttggccgcct gctcggggcc gagcagttga
      841 gcgacgtggt gtttgttgac caatccccca tcggcaaaac cgcccgctcc aacccggtga
      901 gctttgtcgg tgcctgggac accatccgcg agatttttgc caacgccccg ctgtccaagc
      961 aacgtggcta caccagcagc aaattcagct tcaacaacgg cgatggccgt tgcccgacct
     1021 gtggcggctc cggcttcgag catgtcgaaa tgcaattttt gagcgacgtc tatctgcgct
     1081 gccccgactg cgacggcaaa cgttaccgcc cggaaatcct ggaaatcacc atcgagcgca
     1141 ccgcccccgc cagcaccagc ccggttcccg aaccggccag agcaaatgca aacccaaacc
     1201 ccgcgacata tccaggcact caagctggcg cagatgtagc aaacttgggg cagaacacca
     1261 attcgactcc acctgctcaa agcaccgcag ttgcagcaaa tttggggtcg gatcccaatt
     1321 cggcccaaca cagttcaagc aataaccaag cccgcgccga attgggctta gaccccaatt
     1381 ttgcggccca caccgccgcc gccaaaagca ccagcaccac ctgccacctc aacgtcgccg
     1441 acgtgctgaa cctcaccgtc agccaggcgg tagccctctt caaaaacgac cgtgaagtca
     1501 tccgcgccct gcaaccgatc ctcgatgtcg gcctggatta cgtcaaactg ggccagcccg
     1561 tgcccacctt gagcggcggc gaagcgcagc gcctcaagct tgccggtttc ctggccggtg
     1621 ccgccaaaag cagcagcgcc agccgccagg cgctggccag gcgcggcgtg ttgttcatgt
     1681 tcgacgagcc caccaccggg ctgcactttg acgacatcgc caaactcatg cgcgccctgc
     1741 gcaaattgct cgatgccggc cattccctgc tgatcatcga gcacaacctg gacgtgatcc
     1801 gcgccgcaga ctggctgatt gaccttggcc ccgaaggcgg cgaggccggt ggcgaggtgg
     1861 tggcctacgg cacacccgaa gacctgatgc tgcacgccag ctcgcacacc gcgctggcgc
     1921 tgcgcgacta cgcggcacaa atgggcgtgc tgcatggggt gcaggacgtc tcgaacaatc
     1981 ggggtcagag tcgaatttat ccagatgtga caccgtggca agagccggtt ctgtcattgg
     2041 cgcaaaagcc gaaatccaca ccaccactgg atctgcgcct gcgcgggaat aacgatacgc
     2101 tggctcaccc ggtcaccgaa atgctgcaac aaacagagct atcagcgcaa acacagcaag
     2161 ctctggaggc cgaaaagacc ccaaaggttc agccacctga gcgattccaa tcaacgctat
     2221 tcagttcaga gctgccagcg cacagccagc aagcgccaga ggccaaaaac agcatacaaa
     2281 tcatcaacgc ccgcgagcac aacctgcagt ccctcagcgt caacattccg cgcggcaaat
     2341 tcaccgtggt cactggcgtg tcaggctcgg gcaaatcaac gctggcgttt gacatcctgt
     2401 tcaacgaagg ccagcggcgc tacctggagt cgctcaacgc ctacgcccgc tcgatcgtgc
     2461 agcccgcagg gcggcccgag gttgatgcgg tctatggcat tcctccgacg gtggccatcg
     2521 agcagcgcct gagccgtggc gggcgcaagt caacggtcgg caccaccacc gaggtctggc
     2581 actttttgcg cttgttgtac gtcaagctcg gcacccagca ctgcatccac gacggcacgg
     2641 cagtggcacc gcaaacgccc gacagcatcg ccgcgcagtt gctgaaaaat ttccgtggcc
     2701 agcacatcgg cctgctggcg ccgctggtga tgaaccgcaa gggcgtctac accgaactgg
     2761 ccgactgggc caggccacgc ggcttcaccc atttgcgcgt cgatggcaac tttttgccaa
     2821 ccacaaattt cccacgcctg gaccgcttca aggaacacac gatcgagctg ccggtgttca
     2881 gcctggacgt gttgccggaa aaagaagcgc tgttgcgcca ggcgctggcc agcacgctcg
     2941 cccatggcaa gggtgtgctg catgtgctga gccacctcga cggcctgagc gaggcaatgc
     3001 aagcaggcac acccaccgca aacatcggcc acctgagcgt gttttccacc ctgcgcgcct
     3061 gcccactgtg caacaccagc tacgccgaac tcgacccccg gctgttttcc tacaacagca
     3121 aacacggttg gtgcccggac tgcgtcggca ccggagtcaa actgtccaaa gagcagcgcc
     3181 tggtgtttga cgactcggtc aaagacgacg acaacaaggg ccgcgagcag acctttgccg
     3241 aagccgatgt ggaggacctg gccgataccg tttgcccaag ctgccacggc agtcgcctta
     3301 acgccacggc acgagcggtg aagtttggcg cggcagaccc tgagcgcgtc atggccagtg
     3361 acgggtcaaa aatccagttg atacccacac gcatcagtcg cgcgacaaac cctgagcgca
     3421 acctggccag tgaaggtgtt gaccgaacca gcgccaagcc accctccggt tctgccccca
     3481 aaggcgagca agagtcagac caaatgccgt cttccaaaga tgggaatgag tcataccgat
     3541 tgccatctgc cacagatggg aatgagccaa accgattgcc atctgccata gatgggaatg
     3601 agccataccc attgccatct gccacagatg ggaacgagcc ataccgattg ccttctgcca
     3661 cagatgggaa cgagccatac cgattgcctt tctccaaagg cgaccagcag tcagaccaga
     3721 cggcggccga agcaggcttg ccgccttcgg cgcaacaggc gccatcgcat tcgtcggcag
     3781 atagccagac gcttgcaggt atggccctgc gccccctggc agcagattgg ggtgacggtt
     3841 ttgcgatcac agaactggcg cgcctgagcg tgaccgacat gcaccggtgg atgcaggcct
     3901 tgcaggagcc gggccgcatg agccagcgcg aagtcggcat cgcccgcgac ctgattcctg
     3961 aaatcaaagg ccgactggcg tttctggagc aggttggcct gggctacctg acgctgaacc
     4021 ggggcgcgcc aaccctgagc ggcggcgaag cgcaacgcat ccgcctggcc gcgcagcttg
     4081 gcagcaatct gcagggcgtg tgttacgtgc ttgacgagcc gaccattggc ctgcacccgc
     4141 gcgacaacca gattttgctc ggcgcgctgc aacatttaag tgcccagggc aacacgctgg
     4201 tggtagtcga acacgacgaa gacaccatcc gccacgccga ccacatcatc gacatcggcc
     4261 caagtgccgg caaacgcggt ggccgcctgg tcgcccaagg ccaggtggca gacatccaaa
     4321 gcgccgcaga ttcgcaaacc ggccgctacc tgctgcacgc catgcgccat ccgctacagc
     4381 caaggcgctt cgtagatgct tcaaataata tagctgccag cgcatatagc acgggctccg
     4441 cagcccaaaa tcatcaaaat atcgagaccc aggccgggtc acagccgctt gccaccacag
     4501 acggaacatc caccacagca gtgaatttgg cacccgccga aacaatcaac gtcgtagtga
     4561 atttggggtc ggatcccaat ttcccaccca agccgcccaa acaaaaaccc ggaccttcca
     4621 ggaaattggg ctctgacccc aatttcacgg gccaagagcg ccagccgccc cagccgccac
     4681 agcaaccgca gtggatcacc atcacccgcg ccagcctgca caacctgcaa gccattactg
     4741 cccggttccc gctcaagcgc ctggtagccg tcaccggcgt cagcggctcg ggcaaatcga
     4801 ccctggcgcg cg
//
LOCUS       DAOSLL010000360         4812 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15717_length_4812_cov_4.083666, whole genome shotgun sequence.
ACCESSION   DAOSLL010000360 DAOSLL010000000
VERSION     DAOSLL010000360.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15717_length_4812_cov_4.083666"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="PLB25_14640"
     CDS             complement(<1..556)
                     /locus_tag="PLB25_14640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007584526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HOE42845.1"
                     /translation="MLQLSSAGQQAINDIAQRHGFSFDAVLSMLGSVINGNGSMAQFS
                     HPEFGGSGQWMQGGMTMIGDMFNGYLKGRVDNLCAELARLISNQPGLLRSGSFQSQSQ
                     NGSTQVNNWGGGDSGSVTGNGNSLFVPAPVQPDWWGPDLRWPNSSGGQNGMRYAWFSQ
                     ARRLAIESNGQVVIYDTQDHQIGGV"
     gene            complement(1022..3064)
                     /locus_tag="PLB25_14645"
     CDS             complement(1022..3064)
                     /locus_tag="PLB25_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOE42846.1"
                     /translation="MVSTEQVRAELMAYAPQGVEPGATIWVGLQLMHQPHWHSYWKNS
                     GDSGQPTLLNWTLPTGVLAGDIAWPVPKKLPIGNLANYGYDGSVLLPVPLTITPDFKP
                     SPIAPELEVKLQASWLVCKEECVPQDGEFVLRLPVKGSTALNAPEFEATFKATPQPIG
                     NQSSVTIDGNRLQVSVTGLPAALQGQKLELFPETPEVIDPAANWTQSWQGALWTATLT
                     QASHRSASPTVMPLVLVADLAGQRSGFVTKAKVSGNWPAQPLPGALITPSPVSAAPAA
                     LPMSFWMALLGALLGGMLLNLMPCVFPVLAIKVVSFTRNADDQRGHRVSGLAYSAGVV
                     LSFLTLGALMVGLRSAGEQLGWGFQLQSPAVVAALAALFAVLGLNLAGLFEFGQFLPN
                     RLATLEAKNPAVNAFLSGVLAVLIASPCTAPFMGASLGLALSLPALQALLIFAAIGIG
                     MATPYLAASWSPALARLLPRPGAWMDTFRQAMAFPMFATVVWLVWVLGQQAGIDAAGS
                     LLALLLGLVFVIWTMHLRTHRRLPIAIFAIATFACLIWATGKNIVHFEAPPSAVASAA
                     RWQPWSPDKVNQALATGVPVFVDFTAAWCVTCQYNKKTTLANPDLLADFEAKKVALLR
                     ADWTRRDPGITTALTQLGRNGVPVYVLYQTGKPPVLLSEIPSVAEVRAALAALAVL"
     gene            3642..>4812
                     /locus_tag="PLB25_14650"
     CDS             3642..>4812
                     /locus_tag="PLB25_14650"
                     /inference="COORDINATES: protein motif:HMM:NF023277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3369 domain-containing protein"
                     /protein_id="HOE42847.1"
                     /translation="MTSDLIEFVDEEIHVPGAHASRQQCFWRVLIIDDDPDVHQATLF
                     SLDRVELFGRRLFFLHAHSAEEARKLLLAETELALILLDVVMETPAAGLDLVGFIRNT
                     AMLKSARIVLRTGQPGYAPEHDTILRYDINDYKTKSELTHHKLLTTVATAIRSYQQIC
                     TIEANRKGLELIVSASASLIETQGVQTFAAGVMTQLAALLGTQPEALICAQGECGSAE
                     YTVLAAAGQFASLINRPLEDISNAPVRDLLLQVLGSALSHYGTNETVLYFGQKDGYDM
                     AAYIGAAIAAQDIDFQLLKVFSVNMSVCLRNLALLHRLRTDAYVDSLLHLPNRARFIE
                     EIANCVRSGQQRYALAVVDIDDFASVNDLMGQAYGDELLRAYACQLTRQLGSELVL"
BASE COUNT         1026 a         1381 c         1429 g          976 t
ORIGIN      
        1 acacgccgcc aatctggtgg tcttgcgtgt cataaatgac gacctggcca ttggactcaa
       61 tggccagtcg gcgggcctga gaaaaccagg cgtagcgcat gccattttga ccgcctgagc
      121 tgttgggcca gcgcaggtcc gggccccacc agtctggctg caccggtgct ggtacaaaca
      181 gactgttgcc gttgccagtg acgctgccag agtcgccacc accccagttg ttaacctgcg
      241 tgctgccatt ttggctctgg ctttgaaaac tgccactgcg cagcaggccc ggctggttgc
      301 tgatcagtcg cgccagctcg gcgcaaaggt tgtcaacccg gcctttgagg tagccgttga
      361 acatgtcccc aatcatcgtc atgccacctt gcatccactg cccggaacca ccgaactcgg
      421 ggtgactgaa ctgggccatg ctgccattgc cgttgataac cgacccaagc attgacagca
      481 ccgcgtcgaa gctgaagccg tggcgctggg cgatgtcgtt gatggcttgt tggccggctg
      541 aagagagttg aagcataaaa cggactaaaa attgagagga atggtgacgg acaagtatag
      601 gtgggggcta atgcaaaggc gttttgtcct catcgcatct ccgccattcc cgctttggct
      661 tcgatgacgg ttgatccacc acttcaagaa caatcgaatc gacccagatt cacacgccag
      721 gagcctgcca gcctgatggg ccgggcagca agaatcaacc cacagcaagt tcatttttcg
      781 ccgcgcttac aggctcatgg tccggcgatg ggtcacaagg tcggcacaaa aacgggccgc
      841 agtgatcggt tcgcagccgt tctgcgagtt cgacaggctc ccggctcttt ttttggatca
      901 ttcgtgtcgg gggatcacgc cgcaaacatt gcacggcaag gcaagatacc aggcacatgg
      961 atggtgtggg gcaagaatca tgcaccccat ggctgcagca acgcccgcat gaaggctgca
     1021 gtcacaaaac ggccaaggct gccagcgccg cccgcacttc agccactgac ggtatttcag
     1081 acagcagcac cggtggtttg ccggtctgat acagcacata caccggcacg ccattgcgcc
     1141 ccaactgagt cagcgcggtg gtgatgcctg ggtcacggcg ggtccagtcg gcgcgcagca
     1201 gggcaacttt tttggcttca aaatcggcca gtagatccgg attggccagg gtggtctttt
     1261 tgttgtattg gcaagtcacg caccaggcag cggtgaaatc gacaaacact ggcacccccg
     1321 tggccagcgc ctgattgacc ttgtccgggc tccagggctg ccagcgcgca gcactcgcca
     1381 cggcactggg gggtgcctcg aaatggacaa tatttttgcc tgtagcccat atcagacagg
     1441 cgaaggtagc tattgcaaat atagcaatcg gcaagcgcct gtgggtccgc aagtgcatcg
     1501 tccagatgac aaaaaccaga cccagcagca acgccagcaa agatccggca gcgtcaatgc
     1561 cggcttgctg gcccaacacc cagaccagcc agacgacggt ggcaaacatc ggaaaagcca
     1621 tggcctggcg gaaggtgtcc atccaggcac cgggtcgtgg cagcaagcgc gccagtgccg
     1681 gggaccagct tgccgccaaa tagggcgtcg ccatgccgat cccgatggcg gcgaatatca
     1741 gcagcgcctg cagcgcgggc aaactcagcg ccagcccaag tgacgcaccc atgaagggtg
     1801 cggtgcaggg tgacgcaatc agcaccgcca gaacgccgga caaaaaggca ttgactgccg
     1861 ggtttttggc ctccagtgtg gccaggcggt tgggcaaaaa ctgaccaaat tcaaacagcc
     1921 ccgccagatt caggcccagc accgcgaaca atgcggcaag cgctgccacc acggcgggtg
     1981 actgcaactg aaaaccccag ccgagttgtt caccggcgct gcgcaggccc accatcagcg
     2041 cacccagcgt cagaaacgac aacaccacac cggcgctgta ggccaggccg ctgacccggt
     2101 ggccgcgctg gtcatccgcg ttccgggtaa aactcaccac cttgatcgcc agcaccggaa
     2161 acacgcaggg catcaggttc aacaacatcc cacccagcaa ggcaccgagc aatgccatcc
     2221 agaaactcat cggcaaggcg gcgggcgctg ccgacaccgg gctcggcgtt atcagagcgc
     2281 cgggcagcgg ctgggcaggc cagttgccgc tgactttggc cttggtgaca aagccgctgc
     2341 gctgtcctgc caaatcggcc accagcacca gcggcatgac cgtcgggctg gcactgcggt
     2401 ggctggcctg ggtcagcgtg gcggtccaca gcgcgccctg ccagctttgt gtccagttgg
     2461 cagcggggtc gatcacctcc ggggtttccg gaaaaagctc cagcttctgg ccctgcagcg
     2521 cagcgggcag cccggtgacc gacacctgga gcctgttgcc atcaatggtg acactgcttt
     2581 gattgccaat gggctgtggc gtggccttga aagtcgcttc aaattcgggc gcattgagcg
     2641 cggtggagcc tttgaccggc agtcgcaaca caaattcgcc gtcctgcggc acacattcct
     2701 ctttgcagac cagccaactg gcctgcagtt tgacctccag ctcgggcgct atgggtgaag
     2761 gcttgaaatc aggcgtaatg gtcagcggca ctggcagcag caccgagccg tcatagccgt
     2821 agttggccag gttgccaatt ggcagcttct tgggcaccgg ccaggcgatg tcaccggcca
     2881 gcacgccggt cggcagcgtc cagttcaaga gtgtgggctg acctgagtca cccgaatttt
     2941 tccagtaact gtgccagtgc ggctgatgca tgagttgcag gccgacccag atggttgcac
     3001 caggttcgac accttgcggg gcataggcca tcaactcggc gcgcacctgc tcggtgctga
     3061 ccactgattt caatgaattt tgggctccag accatacagg tattgcgcaa gtagctatca
     3121 aaaagtaagc tatcagcggg cgacaacagc ggtacagaaa ggtcatggag cggtaggact
     3181 caggttaagg cctgaagttc ctcaggtgtg gtttgatcag gtcggcagtc taactggcat
     3241 gccagcaatg tgccctgaat ggtgaagttg gttgcgggcg gcttcatgtc aagcgctcaa
     3301 atacccgttt gtgctgggtg atctgacgca aaaataatga ataatgtgcc gaatccattg
     3361 ccaaatcatc gatgttgttg ttttctcgcc ttaccttgcg tttacaaggc ctgcggcagg
     3421 gagcttagac acgaatgatt cagaaatgga atgaggatca ccagcccccg gttttgtcgg
     3481 caatgcgcgt gctggcctga ttggtgggag cagcaagctg aggatggctt ctaggtcaat
     3541 ttcttcacca gttgcaccgg gccatggccg ttggcatttt cagccgtgaa ttgaaggcaa
     3601 ctcattgggg aagactcatg aatacggatg ctggagactc agtgacgagc gatttgattg
     3661 agttcgttga cgaggaaatt cacgttccag gtgcgcatgc atcgcgtcag cagtgctttt
     3721 ggcgcgtctt gatcattgac gatgaccctg atgtgcatca ggcaaccctg ttttcgcttg
     3781 atcgagttga actgtttggc cgacggctgt tttttctgca tgcgcattcg gctgaagaag
     3841 cgcgcaaact gttgctggca gaaaccgagc tggcgctgat cttgcttgat gtggtgatgg
     3901 aaacgcccgc tgccgggctt gacttggtgg gctttatccg caacacggcg atgctgaaaa
     3961 gtgcccggat cgtgctgcgc accggccagc ccggctatgc gccagagcat gacacaattt
     4021 tgcgctacga catcaatgac tacaagacca agtccgagtt gacccaccat aagctgctga
     4081 ccacggtggc aactgcgatc cggtcttacc agcaaatctg caccatcgag gccaatcgca
     4141 aaggtctgga actgatcgtc agtgccagcg ccagcctgat cgaaacacag ggtgtgcaaa
     4201 cctttgctgc tggtgttatg acacagcttg ccgccttgct ggggacgcag cctgaggcgc
     4261 tgatctgcgc ccagggtgag tgtggtagcg ccgaatacac cgtgctggcc gcagccgggc
     4321 agtttgccag tttgattaac cggccactgg aagatatttc caacgcacca gtgcgtgatt
     4381 tgctgctgca ggtgctgggt tcggcgctca gccactacgg cacgaatgag accgtgctgt
     4441 atttcggcca gaaagacggc tatgacatgg ccgcttatat cggggcagcc attgcagccc
     4501 aggacattga ttttcaattg ctcaaggttt tttcggtcaa catgagcgtc tgtctgcgca
     4561 atctggcttt gttgcaccgt ttgcgcaccg atgcttatgt cgactcgttg ctgcatttgc
     4621 ccaatcgggc tcgcttcatt gaggaaattg ccaactgcgt tcggtcaggc cagcaacgct
     4681 atgcgctggc ggtggtcgat atcgacgatt ttgcctcggt caacgatctg atgggtcagg
     4741 cttacggtga cgagctgttg cgggcctatg cctgccaact gaccagacaa ctgggctcgg
     4801 aactggtgtt gg
//
LOCUS       DAOSLL010000361         4800 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15778_length_4800_cov_4.471233, whole genome shotgun sequence.
ACCESSION   DAOSLL010000361 DAOSLL010000000
VERSION     DAOSLL010000361.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15778_length_4800_cov_4.471233"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PLB25_14655"
     CDS             <1..309
                     /locus_tag="PLB25_14655"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE42848.1"
                     /translation="NSQDGSAAKGGDLGWSNPGMFVPEFEDALNRLAPGEISDPVVSR
                     FGVHLIQLEERRKTVLSPEQQREAIRAMLREKKIVEAYQTWAQDIRSRAYVDIREPPL
                     "
     gene            320..1108
                     /gene="rsmA"
                     /locus_tag="PLB25_14660"
     CDS             320..1108
                     /gene="rsmA"
                     /locus_tag="PLB25_14660"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HOE42849.1"
                     /translation="MKVGKHVPRKRFGQHFLTDCGIIDAIVAAIDPLPGQFIVEIGPG
                     LAALTQPLVERLGALTVIELDRDLAQRLRRHGQLSVLESDVLKVDFALLAQALCATKI
                     RVTGNLPYNISTPILFHLLQFVEVIEDQHFMLQKEVIDRMVASPHTAAYGRLSVMLQW
                     RYEMENVLFVPPGSFEPPPRVDSAVVRMLPRANSVALDVRLLSELVQVAFSQRRKLLR
                     HTLGRWLEAKRIGDSFNVQRRAEEVSVEEYLALALTAQEAEKKV"
     gene            complement(1145..1366)
                     /locus_tag="PLB25_14665"
     CDS             complement(1145..1366)
                     /locus_tag="PLB25_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42850.1"
                     /translation="MFLFFSVARSAYTSQAERANEASDTHGDSTTGNARIELLTESGE
                     RMPTDKLLDISPQALRMSSPFNAGYWMTA"
     gene            complement(1345..1785)
                     /locus_tag="PLB25_14670"
     CDS             complement(1345..1785)
                     /locus_tag="PLB25_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HOE42851.1"
                     /translation="MPLDQVTAPLPGTADTPLRGVRPNIVQSIRAFRVQELQEAAQAL
                     GHHFLYANLANAQSKQDVLDLISQQFILTVAVGKNFDSLYDSMTDPVHKSGPQPGFVA
                     VLEHIPANAKFDKEAREQLLDIFRDAADYWGDRKTPFRCFYSFQ"
     gene            complement(1824..2216)
                     /locus_tag="PLB25_14675"
     CDS             complement(1824..2216)
                     /locus_tag="PLB25_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HOE42852.1"
                     /translation="MSRFVNVKPVLTGLCLAAAMVLGLAQAKAPVQVDVQAAISLAEL
                     PKQGVQVFSLIRQGGPFMSEKDGTVFGNRERLLPVQKRGYYREYTVPTPGLKHRGVKR
                     IVCGGQPRLPDVCYYTADHYASFRQIVP"
     gene            3001..4137
                     /locus_tag="PLB25_14680"
     CDS             3001..4137
                     /locus_tag="PLB25_14680"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HOE42853.1"
                     /translation="MNAKAIADQDAWISPLNANTTDRTGATDNERIQDITVLPPPEHL
                     IRFFPIHGTQVEMMISTTRRNIHNIMTGKDDRLLVIIGPCSIHDPAAAMDYAHRLLAQ
                     RQKYADSLEIVMRVYFEKPRTTIGWKGLINDPYLDESFRIDEGLRMARQLLIEINRLG
                     LPAGSEFLDVISPQYLGDLISWGAIGARTTESQVHRELASGLSAPIGFKNGTDGNIQI
                     ATDAIHAAARGHHFLSVHKNGKVAIVQTKGNQDCHAILRGGKTPNYDAASVAAACAEL
                     EKSKLRATLMVDCSHANSGRRHEKQLDVARDIATQIASGSRRVFGVMVESHLMAGAQK
                     FTPGKDQTDRLTYGQSITDACLGWEQSLIALELLSQAVLARRAG"
     gene            complement(4278..>4800)
                     /locus_tag="PLB25_14685"
     CDS             complement(4278..>4800)
                     /locus_tag="PLB25_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42854.1"
                     /translation="ISKRPVFIAFAALSLLLYAKDPLGTFLIPGDWSAVIYVPIIGFV
                     VGMLIYINRGEIDCKISYAIIAFILYIWLKNHQWVHLIFAVTIGYVTLTLGFHQWLQL
                     HISLPNDYSYGIYLYAFPVQQVITRTVSVQSPMLLFVLTMLLTVPLAMISWHAVEKPA
                     LRLKRFFVPIPRR"
BASE COUNT         1168 a         1277 c         1305 g         1050 t
ORIGIN      
        1 aactcgcagg atggcagcgc tgccaagggc ggcgatctgg gttggtcgaa ccccggcatg
       61 ttcgtaccgg aatttgagga tgccttgaat cgcttagctc ccggtgaaat cagcgacccg
      121 gtggtatctc gttttggtgt ccacctgatc cagttggaag agcgccgtaa aaccgtcctc
      181 agccctgaac agcagcgcga agccattcgc gcgatgctgc gcgaaaagaa aatcgttgag
      241 gcctatcaaa cctgggctca ggatatccgc agtcgcgcct acgttgacat tcgcgagcca
      301 ccgctttgaa ggcctgcgct tgaaagtcgg aaaacacgtt ccccgcaaac gttttgggca
      361 acatttcctg actgattgcg gcatcatcga tgccatcgtc gctgcgattg atccactccc
      421 tggccagttc attgtcgaaa ttggccccgg cctggcggcg ctgacgcagc ctctggtgga
      481 gcgactggga gctttgacgg tgattgagct ggaccgcgat ttggcgcagc gcctgcgtcg
      541 ccatggtcag ctctcagtcc ttgagtcaga tgtgttgaaa gtcgattttg cgctactggc
      601 acaagcattg tgtgctacaa aaattagagt aacagggaac ttgccctata acatttccac
      661 accgatcctg tttcacttgc tgcagtttgt cgaggtcatt gaagatcaac atttcatgct
      721 gcaaaaggaa gtcatcgacc gcatggtggc atcaccgcac actgccgcct atggacgtct
      781 gagcgtgatg ctgcaatggc gctatgagat ggaaaatgtg ctgtttgttc cgccggggag
      841 ttttgagcca ccgccccgtg tcgatagcgc cgtggtgcgc atgttgccgc gcgccaactc
      901 agtggcgctc gatgtgcgcc tgctcagtga actggtgcag gtcgctttca gtcagcggcg
      961 caaactgctg cgccacacct tgggacgctg gctggaagcg aaacgtattg gcgacagctt
     1021 caatgtacag cgtcgcgcag aagaagtgtc agttgaggag tacctggcgc tggctctgac
     1081 ggcgcaagaa gccgaaaaaa aagtctgact agtcagactt ttttagcaat cctggccgga
     1141 tgacttacgc tgtcatccag taacccgcat tgaaggggct gctcatgcgc agcgcttgtg
     1201 gagaaatgtc gagcaacttg tcggtcggca tcctttcgcc gctttccgtc agcaattcga
     1261 tacgtgcgtt accggtggtc gaatcaccat gggtgtcact tgcctcgttc gcccgttctg
     1321 cttggctggt gtatgcagaa cgggctactg aaaagaatag aaacacctga agggcgtttt
     1381 ccggtccccc cagtaatcgg cagcgtcgcg gaaaatatcg agtagttgct cgcgggcctc
     1441 cttgtcgaat ttggcattgg ccggaatatg ttccagcact gccacaaacc caggttgtgg
     1501 acctgacttg tgcaccgggt cagtcatgct gtcgtaaagc gagtcaaaat tcttgccgac
     1561 cgcgacggtc aggatgaact gctgactgat caggtccagg acatcctgct tactctgtgc
     1621 attggccaag ttggcgtaca aaaaatggtg tccgagcgcc tgagcggctt cctgcagttc
     1681 ctgtacccga aaagcccgaa tcgactgaac gatgttcggg cgtactccgc gcaacggcgt
     1741 atcggcagtg cccggtaacg gcgcagttac ttggtcaagt ggcattttca tttccctttt
     1801 ctgttcaaaa taacaattat gtgttaaggc acaatttggc gaaagctcgc ataatgatcg
     1861 gcggtgtaat aacaaacatc tggcaggcgg ggctggcctc cacatacaat tctcttgact
     1921 cctcgatgct tcaagcctgg tgtcggtacg gtgtattcgc ggtaatagcc acgtttttgg
     1981 actggcagca agcgctcccg attcccaaac accgtaccat ctttctcgct catgaacggc
     2041 ccaccctgac gtatcaggga gaacacttgg acaccctgtt taggcaactc agccaggctg
     2101 atagcggctt gtacgtcaac ttgaactggt gctttggcct gagccaaacc cagaaccatc
     2161 gcagcagcca aacataagcc agtgagcacc ggcttgacgt tcacaaatct cgacatagca
     2221 aacatttcca ataaatccgc gggtaaaccc gatctttcaa gcgcgcaagt gtcggtgatc
     2281 tgacgtaaaa atgcaagctt gtggtcaatc aggtgggtga ttcaaagtga gtgcgccggt
     2341 aaaaaccggc gagctgcaaa ggcgtagagt cccaaacagt caagagcagg gcattgcttg
     2401 gtttccacca cctgattgcg ccgtcatcag caccttgtca tcaggggacg gatcattgcg
     2461 gcgatgtttg gccatgatct ttgtcaggtc aggggtcaaa gagggaacgg gtgaagatgc
     2521 ggggaaggtg gatcgcggcg tacttgcgac agtcagctca gagggcagca tggccggaat
     2581 tctggtgagc agccaacagg gcagtcggct cccattgtcg tcacggcggg gatggaccgc
     2641 ggctgcggga tagggggcaa gatcgagagt tccaaattac tcccaaatag atagctgact
     2701 atgtagtcca ggtatgggat ggagtcacaa aaggcgtaat aattatcagc aaacacggcg
     2761 aagcagtctg gtgacttgac ctgtgccacc cgtgtcaggc gagcgctcac ctgactgtgc
     2821 tacagtccct tcccatgaat tcggtcaagg ttttctttag ctacttttgg ttctcaagcg
     2881 cctgcggcgg tagagagttt tgaacgtacc caacgaaaac gtcctgattc ctcaaaaacc
     2941 gccggccaac ccggcggttt ttttatgcct gtctgattca accctgcaag gaaatacccg
     3001 atgaatgcca aagctattgc tgaccaagat gcctggatca gtcctttgaa tgccaacacc
     3061 accgaccgca ccggcgcgac cgacaacgaa cgcatccagg acatcaccgt gttaccccct
     3121 ccagagcatc tgatccggtt tttcccgatt catggcacac aggtcgaaat gatgatcagc
     3181 accacccggc gcaacattca caacatcatg acgggcaagg acgaccgcct gctggtgatc
     3241 attggcccct gttcaattca cgatccggcc gccgcgatgg attacgccca ccggctcttg
     3301 gcccagcgcc agaagtatgc ggattcactg gaaatcgtca tgcgtgtgta tttcgagaag
     3361 ccccgcacca caattggctg gaagggattg atcaacgacc cttatctcga cgaaagcttc
     3421 cgcattgacg aaggcttgcg aatggcgcgc cagttgctga tcgagatcaa ccgactgggc
     3481 ctgccggcgg gcagcgagtt tctggacgtg atttcgccgc aatacctggg tgacctgatt
     3541 tcctggggcg cgattggtgc ccgcaccaca gaaagccagg tacatcgcga gttagcttcc
     3601 ggcctgtcgg caccgattgg tttcaagaac ggaaccgacg gtaatattca aattgccacc
     3661 gatgcgattc acgcagccgc gagggggcat cactttttgt cggtacataa aaatggcaag
     3721 gtcgccattg ttcagaccaa gggcaaccaa gactgccacg ctatcttgcg cggtggaaag
     3781 acccccaact atgacgcagc cagtgtggct gcggcctgtg ccgagttgga aaagtcgaaa
     3841 ctgcgagcca ccttgatggt ggactgcagc cacgccaaca gcggtcggcg gcacgaaaaa
     3901 caactcgatg tggcgcgcga cattgcaacc cagattgcca gtgggtccag gcgtgtgttc
     3961 ggcgtgatgg tcgaaagcca tttgatggca ggagcccaga agttcacccc aggcaaggac
     4021 cagaccgacc ggctgaccta cggccaaagc atcaccgatg cttgcctagg ctgggagcag
     4081 tcactgatag cgctggaatt gttgtctcag gccgtgttgg cgcgtcgggc cggataaagt
     4141 cagagcctgc agcagggctt ccgaagcctc acagaaccgt cgaacaggca aaaatatttg
     4201 gcaaaaattg ccgctagccc acgttccata agcgctgata gctactcgtt atatagcata
     4261 gaaatcctct caagaggtta tcttcgagga attggcacaa aaaacctctt cagtctgagt
     4321 gctggttttt ccaccgcatg ccaagaaatc attgccaatg gaacagtgag cagcatggtc
     4381 agcacaaaca gcaacattgg tgattgaaca gacactgtgc gggtaatcac ttgctgaacc
     4441 ggaaatgcat acaagtaaat gccgtatgag tagtcattcg gcagagaaat atgaagctgg
     4501 agccactggt gaaacccaag cgtcaaggtt acatatccaa tcgtaacggc aaagataagg
     4561 tgaacccatt gatgattctt gagccatata tataggatga acgcaatgat tgcatacgaa
     4621 atcttgcaat caatctcgcc acggttaata taaatcaaca tcccaactac aaaaccaatt
     4681 atcggaacat aaataacagc agaccagtca cctggaatca aaaaggtacc aagcgggtcc
     4741 ttggcataaa gcagcagtga gagcgccgca aatgcaataa ataccgggcg cttcgatatc
//
LOCUS       DAOSLL010000362         4794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15796_length_4794_cov_4.169234, whole genome shotgun sequence.
ACCESSION   DAOSLL010000362 DAOSLL010000000
VERSION     DAOSLL010000362.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15796_length_4794_cov_4.169234"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..1365
                     /locus_tag="PLB25_14690"
     CDS             79..1365
                     /locus_tag="PLB25_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HOE42855.1"
                     /translation="MKLFPYAHATHPQWGMAAGLALAQLRAQIALHGYASSPTLGVLY
                     ITDHYASAAPDILAYLSAELPEITDWVGTVGVGIASNNVEYFDEPALAVMLCELPSDQ
                     YRVFSGVAPLGMAFRPHTALVHADAQTADLTELISELAARMDSGYLFGGLSASRSGAL
                     QFAVAGDGNVQGQGAASGVFSGGLSGVAFTAGVGLVSRVTQGCVPLAKAHRVTSADHN
                     VVLALDHQPALEVLLRELKVSMDQPRQVLPAVRAALVGLTAGRDADAAPDLLTVTRTG
                     NFGNDVMVRHIIGLDPGRAGVAVSALVDVGMELAFCRRDVQAARADLTRICAEIREEL
                     EPQEQALETATALAASDAESAPHPLRGIAGAIYVSCAGRGGQHFGGPSAELQIIRRAL
                     GDVPLVGFFAGGEIARSHLYGYTGVLTVFTGNQAAA"
     gene            1962..2912
                     /locus_tag="PLB25_14695"
     CDS             1962..2912
                     /locus_tag="PLB25_14695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE42856.1"
                     /translation="MDTDQTAIAMLLDAPPGRPRLLVVDDQPINIQVMYQIFASQYQV
                     FMATSGQQALDFCHKTPPDLVLLNVVMPGMDGFEVCQALKAAPDTHDIPVIFVTAHTE
                     AVQETRGLDAGAVDFISKPINPAVVRARVKTQLTLKFQSDLMRKLVFRDGLTGVFNRH
                     YFDQQLAMEVARSSRAKSPLALIMLDVDFFKRFNDHYGHQAGDDCLRAIAATLKGSLR
                     RPADLVARYGGEEFVCILPDATFAQAMSMASQLEQNVRHKRIPHLDSQAADAVTISLG
                     VVGVSGDSGRDENALLALADTQLYLAKNTGRARVCGRAIT"
     gene            2974..>4794
                     /locus_tag="PLB25_14700"
     CDS             2974..>4794
                     /locus_tag="PLB25_14700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE42857.1"
                     /translation="MNQSASKISCTLLSGMLRWLPRKLEHQVMLLTSLCLVLSIMGYG
                     YHRATEETANARRSIAAQMIALARNLAAVDVHFMRSNEPHQIEALTLHTATVDGIYSV
                     LVTNINGKPLSEVVNKNGAWSPRYNAAAIAIPANTSPDSVFETRPHSTVHRDFLAGNS
                     GTMSAWHRMGDATNPLGWVRVNYRLDTFDAIASEIQWQAAKAVLLAMLATLSLLSLLL
                     RPTLRALREATEFASQLDTAMGARLKISRSTQEVEALGSALNVVSARLQSEHAELINQ
                     KFALDQHAIVSITDLAGNITYANQRFCDISGYRVEELLGQNHRIVKSDEHSPAMFEDM
                     WLTITRGSVWRGDIKNRKKDGSFYWVNATIVPLTGLDGLPHHYIGIRTDITANKALEL
                     RLQAARDLAESATLAKGHFLANMSHEIRTPMNAVLGMLKLLQNTDLTKRQLDYAHKAE
                     GAARSLLGLLNDILDFSKIDAGKMTLESRPFRLDTLMRDISVIVSANLGAKPVEVLFD
                     IDQATPRVLMGDGLRLQQVLINLSGNAIKFTSDGEVVISIKVTGRSDNEVALRFAVRD
                     SGIGIAHAQQVQLFQPFSQADSSTTRQFGGTGLGLSIVRSL"
BASE COUNT          988 a         1395 c         1405 g         1006 t
ORIGIN      
        1 gctggcaaaa cagataccaa ggccaaccca gacggccaat agcgcctcga cctgtcaaca
       61 aaaagacccg cccggcccat gaaactgttt ccctacgcgc acgcaaccca cccccaatgg
      121 ggcatggccg ctggcttggc gctggcgcag ttgcgggccc agattgcgct tcatggctac
      181 gccagcagtc cgacgctggg cgtgctctac atcacggatc actacgctag cgcggcacca
      241 gacattctgg cgtacctgag tgccgagttg cccgagatca ccgattgggt tggcacggtg
      301 ggcgttggca ttgcgtccaa caatgtcgag tattttgacg agccagcgtt agcggtgatg
      361 ttgtgcgaac tgccaagcga ccagtaccgg gtgttttccg gcgtggcgcc gctgggcatg
      421 gcgtttcggc cccacactgc gctggtccac gccgatgcgc aaaccgccga cctgaccgag
      481 ctgatcagcg aattggccgc gcgcatggat tcgggctatc tgtttggagg cctgtcggcc
      541 agtcgcagcg gtgcgctgca gtttgccgtg gcgggtgacg gcaatgtcca gggccagggt
      601 gcggcaagcg gggtcttcag cggaggcctg agcggcgtgg cctttaccgc cggggttggc
      661 ctggtgtcaa gggtcacgca gggttgcgtg ccactggcca aagcacaccg tgtgacatcc
      721 gctgaccaca atgtggtgtt ggcactggac catcaacctg cgctcgaagt gctgctgcgc
      781 gagctcaagg tgtcaatgga ccagccccgc caggtgttac cggcggtgcg tgccgcgcta
      841 gtcggcttaa cggctggcag agacgcagat gcggcccccg atttgctcac ggtgacgcgc
      901 accggcaact ttggcaacga cgtgatggtg cgccacatca ttggcctgga ccctgggcgc
      961 gccggggtgg cggtgtcggc gctggtcgat gtcggcatgg aattggcgtt ttgtcggcgc
     1021 gatgtgcaag ctgcccgagc tgacctgacg cgcatctgcg ccgaaatccg tgaggaactg
     1081 gagccgcagg agcaggccct ggagacggcc acggcgctgg cagcatctga tgccgaatcg
     1141 gcaccgcatc cgctgcgtgg catcgccggg gccatctatg tgagctgcgc cggacgtggg
     1201 ggccagcatt ttggtggccc gagtgccgaa ctgcagatca tccgccgagc cttgggcgac
     1261 gtgcccttgg tgggtttttt cgccggcggc gagatcgcca gaagtcacct ctatggctac
     1321 accggggtgc tcactgtttt tacgggaaat caggctgcag cctagatgga ataagcgctg
     1381 taagctatat ttacgatagc tctcctgatc tcacatcaag gttcaccaaa acgcctccac
     1441 cgggcaatat cgcgccgggt agccccgtgc cgttcacgtg gccagtgttc gtgtgctgcc
     1501 atcaccttgg cgcaggcctt ggtcagcgca acgctacgcc agctacccgg agcaaaccca
     1561 taaagcggtg tgccagcata cagtgatcgg accaatgttt caccccagct ttgatgagct
     1621 gaaggggcaa tcctccacca tctcaataca attgcggttt gggcagtttg atggcttggc
     1681 caattgagcg tattgaaatg acgtgccctg cgcgctaact tggtcaaagg cggctatcga
     1741 aacaactgtc aaataggcgt tggattcaac tggaccagat aggatgatct agcatgtcaa
     1801 aattcgtaag tgcttgtttt tattgttttt attcaaaaaa agcacagcgc aggaatttac
     1861 ggcagtcgct gcaagcggat ttccctgccg cctgttggcc agggcaagat ggtgatgcct
     1921 ggtgtcgctg ctattgcagt ttgccacgtt gaaacgcgtt catggatacc gaccaaactg
     1981 ccattgccat gctgctcgat gcgcctccgg gcaggccgcg actgctggtg gttgacgatc
     2041 agcccatcaa tattcaagtg atgtaccaga tcttcgcatc gcaatatcag gtgttcatgg
     2101 ccaccagcgg tcagcaggcc ctggattttt gccataaaac accccctgat ctggtcctgc
     2161 ttaatgtcgt gatgcccggc atggatggtt tcgaggtgtg tcaggcgctc aaggcagccc
     2221 cggacaccca tgatattccg gttatttttg tcaccgccca taccgaggca gtgcaggaaa
     2281 cacgcggact tgatgctggt gcggttgact ttatttccaa accgatcaat ccagccgtgg
     2341 tgcgggcacg tgtcaaaacc caactcaccc tcaaattcca gtcggacctc atgcgcaagc
     2401 tggtatttcg ggacggttta accggtgtct tcaaccggca ctattttgac cagcaactcg
     2461 caatggaggt ggcaagatcg tcccgagcca aatcgccact ggcgctgatc atgctggatg
     2521 tcgatttttt caaacgattc aatgaccatt atggtcacca ggccggtgac gattgcttgc
     2581 gagccattgc cgccacgctc aagggcagcc tgcgtcgacc ggctgacctg gtggcgcgct
     2641 acggtggcga agagtttgtc tgcatccttc cagatgccac ttttgcacaa gccatgagca
     2701 tggcatcgca attggagcaa aacgtgcgac acaagcggat tccccacctt gactcacagg
     2761 ctgcggatgc agtgaccatc agccttggcg tggtgggtgt ttcgggtgac tcgggtcgtg
     2821 atgagaatgc cctgctggca ctggctgata cacaactcta tcttgcaaag aacaccggtc
     2881 gtgcccgggt ttgtggccgt gcaataacat gaagggtcat ctacttgcgc cgtctcaggt
     2941 gcccattgct gggtaataga tggccctccg agcatgaacc aaagcgcatc caaaatttcc
     3001 tgcacgctgc tgagtgggat gttgcgctgg ctgccgcgca agctggagca tcaggtcatg
     3061 ctgctgacat cgctgtgtct ggtgctgtcc atcatgggct acggctatca ccgcgcgacc
     3121 gaagaaaccg ccaatgcccg tcgctccatt gcagcgcaaa tgattgcgct ggccaggaac
     3181 ctggccgccg ttgatgttca cttcatgcgc agcaatgagc cccaccaaat tgaggcgctg
     3241 acgctgcaca ccgcaacggt ggacggcatc tattcggtac tggtcaccaa tatcaatggc
     3301 aagccattgt ccgaagtggt taacaaaaac ggcgcctggt cgccgcgcta caacgctgct
     3361 gcgattgcga ttcctgccaa caccagcccc gacagcgttt tcgaaaccag accgcacagc
     3421 acggtgcatc gggactttct ggccggaaac agtggcacca tgagcgcgtg gcatcgcatg
     3481 ggtgatgcca ccaacccctt gggttgggtg cgggtcaact accggcttga cacctttgat
     3541 gccatcgcca gcgagattca gtggcaagcc gccaaagccg tgctgctggc catgctggcg
     3601 accttatccc tattgtcatt gctgctgcgt ccaaccctgc gcgccctgcg ggaggcaact
     3661 gaatttgcca gccagctcga taccgcgatg ggtgccagac tgaagatttc gcgcagcact
     3721 caagaagtcg aagcgctggg aagcgcgctc aacgtggtgt cagcacgcct gcaatcggaa
     3781 catgccgaac tgattaacca gaagttcgca ctggaccagc acgccatcgt cagcatcacc
     3841 gatctggctg gcaacatcac ctacgccaac cagcgctttt gtgacatcag cggttaccgt
     3901 gttgaggaac tgctgggcca aaatcaccgg atcgtcaagt cagacgagca cagcccggcg
     3961 atgttcgaag acatgtggct caccatcacg cgcggcagcg tttggcgcgg cgacatcaag
     4021 aacagaaaaa aagatggcag cttttattgg gtcaatgcca ccattgttcc tttgacgggt
     4081 ctggatggtt tgccgcatca ctacatcgga atccgcaccg acatcactgc caacaaagcg
     4141 cttgagctgc gcttacaggc agcgcgtgat ctggccgaat ccgcgaccct ggcaaagggg
     4201 catttcctgg ccaacatgag tcacgaaatc cgcaccccca tgaacgctgt tctgggcatg
     4261 ctcaagctgc tgcaaaacac cgacctgacc aagcgtcaac tggactacgc ccacaaggcc
     4321 gaaggtgccg cgcgctcctt gctgggcttg ttgaacgata ttctggattt ttcaaaaata
     4381 gatgcgggca agatgaccct ggagtcgcgc ccgtttcgcc ttgacacgct gatgcgcgat
     4441 atttccgtca ttgtgtcagc caacctgggg gccaagccag tcgaggtgct gtttgacatc
     4501 gatcaggcga cacccagagt gctgatgggt gatggcttgc ggctgcaaca ggtgctcatc
     4561 aacctcagcg gcaatgccat caaatttacc tctgatggcg aggtggtgat cagcatcaag
     4621 gtgactgggc gcagcgacaa cgaggttgcg ttgcgatttg ccgttcgcga cagcggcatt
     4681 ggcattgcgc acgcgcagca ggtgcaactg tttcagccgt tttcccaggc cgacagcagc
     4741 accacacgtc agtttggtgg caccgggctt ggcctgtcca ttgtgcgcag cctg
//
LOCUS       DAOSLL010000363         4791 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15809_length_4791_cov_3.957137, whole genome shotgun sequence.
ACCESSION   DAOSLL010000363 DAOSLL010000000
VERSION     DAOSLL010000363.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15809_length_4791_cov_3.957137"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="PLB25_14705"
     CDS             <1..933
                     /locus_tag="PLB25_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE42858.1"
                     /translation="GRTQQAAGEFNQAIATFNKLAAMQPLSPQPHLRLADAHMAAKDK
                     TAAAASLRKALEIKPDLQQAQRGSIMLDVDGKNIQGALSTARTMQKQGPGDAAGYVLE
                     GDINASQKNWDAAATAYRDGLKQVSSVELAVKLHAALLASGKIAEADKYAASWQKDHP
                     NDAGFLLYLADSGIARRDFANSEKTYLNVIKLQPDNAVAYNNLAWVAGQLNKAEAIGY
                     AEKAISIAPNQPEFMDTLAMLLSEKGNYAKALELQTKAVAARPKNQLFKLNLAKIHLK
                     GGKKDLAKKELDELAKLGDKFGGQKEVAELLKGI"
     gene            complement(975..1766)
                     /locus_tag="PLB25_14710"
     CDS             complement(975..1766)
                     /locus_tag="PLB25_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HOE42859.1"
                     /translation="MSLHYFVRLLVACAALFVTSVHADLVKTIAQVKPSVVIVGTYKV
                     TNSPRFSLRGTGFVLAEGNSPDSNLVVTNAHVLQRPAEEDTEAAMVVQVRKGLTDLQM
                     RAVTVLEVDSLHDLALLRFDGPAVPGLVLRNSDTVREGQAVAFMGFPIGGALGFSPVT
                     HRGTVSSIAAAVLPPPTAQHLNAQAIRSLRTGSSFDIFQLDGTAYPGNSGGPLFDPDT
                     AELLGVVNMVLIKGARESALTNPSGISYAIPSRFVAQLLQRYQAR"
     gene            complement(2053..2898)
                     /locus_tag="PLB25_14715"
     CDS             complement(2053..2898)
                     /locus_tag="PLB25_14715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOE42860.1"
                     /translation="MKITVWKQIAQSFAACSLATLGASAFAASDWSWNFATACGGSNN
                     GTYASLTCTSGGSNPNATLTAWSTTKDAGTTKTNSTSGTEFATASLAWYTGGHIGVKN
                     QTGVDEVNPQHSMDNYGQTDMIMFNFGATKVDLDSVSIGWKAYDSDISVLRYTGSLAP
                     VVAGKSIAGLLSDGWVSVGNYANLNSTGTAKAVNAGNVGSSYWLISAYNTNFGAGCDT
                     LSGCSSSSTTSSTSGTNDYVKLLAVAGSRVTETSRVPEPGSLALLGAATLGFVASRRR
                     KLKAA"
     gene            complement(3084..>4791)
                     /gene="prsT"
                     /locus_tag="PLB25_14720"
     CDS             complement(3084..>4791)
                     /gene="prsT"
                     /locus_tag="PLB25_14720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HOE42861.1"
                     /translation="VLESYLKANPADGQAMTLLAGALMSKGQHARASSLMHQALQLTD
                     APEFRSVLGLSLIRGGQAAAGMAELEAAYKKDPRQTPAATALVGYYLRGRQNDKAVAM
                     AESLINQQPGNAGFHNLLGMAKGQSGKLAESKAAFEQALRLNPALVSPKLNLARIDIA
                     TKAYDAAAARLTAILKADEKNTEAMFEMALLSDRRGQAADTQRWLEKATDLSGPKETR
                     WGLALSDFHLRNGRAGPALDAVKKVSAKAPDDLAVLLAYARAQLANGDVTGAKSNLNT
                     ATRVADYNAASQVQIAMLQLAAKNPGGAAYSLEKALSGQPDFLPAQALMTEVELRQGE
                     PANAEKRARDILARHPKLALGHSLLGDIAQSRGQPTQAVEAYRRAYQTEPSTNTFMRL
                     FGALANQDGGKAAIELAQQWTRLHPKDARAQKALANTYARSGQFALAKTSYEILVALT
                     PDDSDALNNLANVLLRLKDPAAVKMAEQAVAKNPNDANAIDTLGWALFQAGQTERALQ
                     YLRDARLRQPGNPEIRYHLAVVLALTGRKAEAINELESALKDGQGFETATEAAALLKA
                     LK"
BASE COUNT         1055 a         1382 c         1345 g         1009 t
ORIGIN      
        1 gggcgcaccc agcaggccgc aggtgaattc aaccaggcca tcgccacctt caacaagctc
       61 gcggcaatgc aacccctgtc cccgcagcca catctgcgcc tggcagacgc gcacatggcc
      121 gccaaagaca aaactgcagc cgctgccagc ctgcgcaagg cgctagagat caagcccgat
      181 ctgcagcagg cgcagcgcgg cagcatcatg cttgacgtcg atggcaaaaa cattcagggg
      241 gcgctgagca ctgcgcgaac catgcaaaag cagggtccgg gcgatgccgc aggctatgtg
      301 ctcgaaggtg acatcaacgc atcccagaag aactgggatg ccgcggccac tgcctaccgc
      361 gatggcctca agcaagtcag ttcggttgaa ctggcggtca agctgcatgc ggcgttgctg
      421 gcctcgggaa aaatcgctga ggctgacaaa tatgctgcaa gctggcaaaa agatcacccc
      481 aacgacgcag gctttttgct gtacctggca gacagtggca tcgctcgcag agatttcgcc
      541 aattctgaaa aaacctattt gaacgtgatc aagttgcagc ccgacaatgc agttgcctac
      601 aacaacctgg cctgggtggc tggccaattg aacaaggccg aggccatcgg ttacgctgaa
      661 aaggccatca gcattgcgcc taaccaaccg gagttcatgg acacgctggc gatgcttctg
      721 tcagaaaagg gcaactatgc caaggcgctt gaattgcaga ccaaagcggt cgcagcgcgg
      781 ccgaaaaacc agttgttcaa actgaacctg gcgaagatcc acctcaaggg tggcaaaaaa
      841 gacctggcca agaaggaact cgacgaattg gccaagttgg gtgacaagtt cggtggccag
      901 aaagaggtgg ctgaactgct caaaggcatt tagttcgagg caaagtctga cccaaaacca
      961 atctgcagat gcgattacct ggcttgatac cgctgcagca attgcgcgac aaatctggac
     1021 ggaattgcgt aactgatgcc tgaggggttg gtcagcgccg attcgcgtgc gcccttgatc
     1081 aacaccatgt tcaccacgcc cagcaattca gcggtgtccg ggtcaaacaa ggggccacca
     1141 ctgttgccgg ggtaggccgt gccgtcaagc tgaaagatgt caaaactgct gcccgtgcgc
     1201 aaactgcgga tggcctgggc attgagatgc tgtgccgtag gcggtggcaa cacagcagca
     1261 gcgatcgaag aaaccgtgcc acggtgggta accggcgaaa agcccaaggc accaccaatg
     1321 ggaaagccca tgaatgccac cgcctggccc tcgcgcacgg tgtcagagtt gcgcagcacc
     1381 aacccaggca ccgctggccc atcaaagcgc agcaaggcca gatcatgcag actgtccacc
     1441 tccagcaccg tgacggcgcg catttgtaaa tcggtcaggc cttttcgcac ctgtaccacc
     1501 atggcggcct cggtgtcttc ctcggccggt ctttgcaaca catgggcgtt tgtcaccacc
     1561 agattgctgt ctggcgagtt gccctcagcg agcacaaaac cggtgccgcg caggctaaaa
     1621 cgcgggctgt tggtgacctt gtaggtgcca acaatcacca ccgagggttt gacctgcgcg
     1681 atggtcttga ccagatccgc gtgcacagaa gtgacaaaca gggccgcgca tgcgaccaga
     1741 aggcggacaa agtaatgcaa gctcatgttt ttccctggtt gtgcccgaag taacgggcga
     1801 atggccgatt ttgcactgtc ggcagaaccg cgacatgggc aggtaagtaa cggtgcaggg
     1861 caactttggt ctgcttccat gttgttaact tgctgtcttt ccacgaacac gcgaatgcgc
     1921 ttgccttgtg cacgttactt cacttataaa tttatagctg acagcacaat ctggataagg
     1981 gctagaggcc aaaaagggat aaaaaaagca ccgggatcag cggtgccttt gtgggttggg
     2041 aaacgaagtg aatcaggcag cttttagctt gcgccgacgg ctcgcaacaa agcccaacgt
     2101 ggcggcaccc agcagagcga gggaaccagg ttcgggaact cggctggtct ctgtgacacg
     2161 actacctgcc actgccagca gtttgacgta atcatttgtc ccgctcgtag aagatgtggt
     2221 tgatgaactt gaacatccgc tgagggtgtc gcaacccgcg ccaaagttgg tgttataggc
     2281 actaatcaac cagtagcttg aacccacatt acctgcattg acagccttgg ccgtgccagt
     2341 cgaattgaga ttagcgtagt tgccaacgga aacccaacca tccgacaaca gtcctgctat
     2401 cgactttccc gcaacaactg gtgccaatga gcccgtgtat ctcagcacac tgatgtcgga
     2461 atcgtaagcc ttccacccaa tgctgacgga gtccaaatcc acttttgttg ctccgaaatt
     2521 aaacatgatc atgtcggttt ggccgtaatt gtccatggag tgttggggat tcacttcatc
     2581 gacaccggtt tgattcttaa cgccaatgtg accaccggta taccaggcaa gacttgcagt
     2641 cgcaaactca gtcccgctag ttgaatttgt ctttgtggta cctgcatctt ttgttgttga
     2701 ccatgcggtt aacgtcgcat tcggatttga accacctgaa gtgcacgtga gactggcata
     2761 agtgccattg ttactgccac cgcaagcagt agcgaaattc caactccaat ccgacgctgc
     2821 aaaagcggat gctccaagag tggccaatga acatgccgca aatgactgcg cgatttgctt
     2881 ccaaacagta attttcataa tcattatcct tgacattttg gctcatctaa acctgcgcta
     2941 gattcaagcg cagtcggttt gattaactaa gcgaaattca tgccaaaaaa ttcgatcatt
     3001 taatatcaac gacttaaatg aatcagcgca aggaagtgaa accgtttgta aaaaacttcg
     3061 acactgttcg ctagcgcaca gacttatttc aacgctttga gtagcgccgc agcttcagtc
     3121 gcggtttcaa atccctgtcc atctttcaat gcggactcca actcgtttat cgcctcagcc
     3181 ttgcgcccag tcagtgccag caccaccgcc aagtggtacc gaatctcagg atttccgggc
     3241 tgcctcagcc gtgcatcgcg caaatattgc aatgcccgtt ccgtctgccc ggcctgaaac
     3301 agcgcccagc ccaaggtatc aatcgcattg gcgtcattcg ggttcttggc cacggcctgc
     3361 tcggccatct tgaccgccgc ggggtctttc aggcgtagca acacattggc cagattgttc
     3421 agcgcatcgc tgtcatccgg ggtcagtgcc accaggattt catagctggt tttggccagc
     3481 gcaaactggc cactgcgggc ataggtgttg gccagcgctt tttgggctcg ggcgtctttg
     3541 gggtggagcc tggtccattg ctgggccaac tcaatcgccg ccttgccgcc atcctgattg
     3601 gccagggcac caaatagccg cataaaggtg ttggtgctcg gttctgtctg atacgcccgg
     3661 cggtaagctt ccaccgcctg ggtcggctgc ccacgtgact gggcaatgtc acccagcagg
     3721 ctgtggccca gcgccagttt ggggtgtctg gccagaatgt cgcgcgcacg cttttcggca
     3781 ttggcaggtt caccctggcg cagttccacc tctgtcatca gcgcctgggc gggcaaaaag
     3841 tcgggttggc ccgacaaggc tttttccagg ctataggccg cgccgcccgg gtttttggct
     3901 gccagttgca gcatggcaat ctgcacctgt gatgctgcgt tgtagtcggc cacccgggtg
     3961 gctgtgttca ggttgctttt ggctccggtc acatcgccgt tggccaattg cgccctggca
     4021 taagccagca gcaccgccag gtcgtccggg gccttggccg aaactttctt gacagcatcc
     4081 aatgccgggc cagcgcgacc gttgcgcaaa tggaagtccg acagcgccag tccccagcgg
     4141 gtttccttcg gacccgagag gtcggtggcc ttttccagcc agcgctgggt gtctgcggcc
     4201 tgaccgcgcc ggtctgacag cagcgccatt tcaaacatgg cctcggtgtt tttttcatct
     4261 gctttcagaa tggcggtcag gcgcgcagcg gcggcatcgt aggctttggt ggcgatgtcg
     4321 atgcgggcca gattcagttt gggcgacacc agcgccgggt tgagcctgag ggcttgctca
     4381 aaagccgctt tggactcggc cagcttgccg ctttgccctt tggccatgcc cagcaggttg
     4441 tggaaaccgg cattgccggg ctgctgattg atcaggctct cagccatcgc cacggccttg
     4501 tcgttctggc gaccacgcag gtagtagccc accagggcgg tagccgcagg ggtctggcga
     4561 gggtcctttt tatacgctgc ttccagttcg gccatgccgg cagccgcctg gccaccgcga
     4621 atcaggctca ggcccagcac cgagcgaaat tccggcgcgt cggtgagctg cagcgcctga
     4681 tgcatcagcg aactggcgcg tgcgtgctgg cctttggaca tcaaagcgcc cgccagcagg
     4741 gtcatggcct gaccatcggc cgggttcgct ttcaggtagc tttccagcac c
//
LOCUS       DAOSLL010000364         4785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15825_length_4785_cov_6.258562, whole genome shotgun sequence.
ACCESSION   DAOSLL010000364 DAOSLL010000000
VERSION     DAOSLL010000364.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15825_length_4785_cov_6.258562"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..106
                     /locus_tag="PLB25_14725"
     CDS             <1..106
                     /locus_tag="PLB25_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOE42862.1"
                     /translation="VRQLSPEQIQGLRRSADHYVANAQRFKTTLNKLA"
     gene            122..1105
                     /locus_tag="PLB25_14730"
     CDS             122..1105
                     /locus_tag="PLB25_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HOE42863.1"
                     /translation="MSEIHKFLFEGLPVRGVLVRLTDAWTEVLARRAANTMHGAYPPP
                     VQNLLGEMTAAAVLMQSNIKFDGALVLQIFGDGPVKLAVVEVQPDFGLRATATVNAAI
                     DATASLSQMVNVGNAGRCAITLDPQNRQPGQQPYQGVVPLFGDAREKLEKLSDVLQHY
                     MLQSEQLDTTLVLAANQQVAAGLLIQRLPMLGSGNLAGAQTANEDQIGINEHYNRIAL
                     LAATLQREELLTLDAEAILHRLFWEEDLRRFEPLQGAGGPHFSCTCSRERVSKMLIGL
                     GQDEAQSILQERGLIEVGCDFCSQQYRFDAVDVSHMFRQTDQTPPASTAVQ"
     gene            1622..1960
                     /locus_tag="PLB25_14735"
     CDS             1622..1960
                     /locus_tag="PLB25_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOE42864.1"
                     /translation="MNRMTHNGYTAQMEFDVEDKIIVGRVIDIDDIITFHGTSVAEFE
                     AVFKAAVDGYIQACQKLGQAAEKSASGRLMLRVSPSIHAAAVKASARSGQSLNKWAEK
                     VLGQAARALP"
     gene            complement(2357..3709)
                     /locus_tag="PLB25_14740"
     CDS             complement(2357..3709)
                     /locus_tag="PLB25_14740"
                     /inference="COORDINATES: protein motif:HMM:NF017773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE42865.1"
                     /translation="MKPFHLHIASTPDASAAVCRWFGTSALRAEGALTLSLALGAETA
                     TLTDDFAIDLAPLLAEVAADGITDAEALAATDEFLPLRDLLAAAGFAELCAALFLFLW
                     RAAQAPTLFALKRGADERVWSDPDRRLVRVWFATNRKPCEGGSPAEPFSADESADTLS
                     YGHCSVFIPKSHRPGSTGSAWWRRWIRLEADDTLALRATVRLARERFWPLLADKLATW
                     WKPGERNVFVLIHGFNVSFAQAAVRAAQIGYDLKLPGEMAFYSWPSKGGVMDYSADEA
                     TIGASVGQIATFLDELSQRSGAERIHLFVHSMGNRGFLAALERLAALRRPALRLGQVF
                     FCAPDEDTRIFADKTQAFPCQAENRTLLVSPDDKAVALSRWKHQADRVGIVPPLRQYD
                     GIETISVTGFGLLDLGHGYFAEAEQVILDMREAIADQKRAGARAIPRVVADHFQIEIR
                     "
     gene            complement(3794..>4785)
                     /locus_tag="PLB25_14745"
     CDS             complement(3794..>4785)
                     /locus_tag="PLB25_14745"
                     /inference="COORDINATES: protein motif:HMM:NF026288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE42866.1"
                     /translation="KTPTVAPAKPGDYLFYLRGLEVDKELHDLRGQGVTEGQLGTLAM
                     AEGDLNEAVQRHQAALALFQRLQEPAMEAVAQHQLGRAFQEAKQWEQAERHYRESAVL
                     KEKRGDLASAARTWNQLAMVCERTGRPEVAETWYRKAIAAMQNPPNGDRGAVCLSNLA
                     ELLQHQPGRLAEAHQLAEEALAIKATLDPGAAEIWKTYSLLAKIAEQQSRPREATDYR
                     RQAREAKRGYAGTAHEMKRYSPLIAAVVLAIGGDAKAQTATEKYMAQLEKAGGENTEL
                     AAALRRILSGDRDEEVLCAGIGLTASMIIETLLHALDDPSSRAALLPQPKPED"
BASE COUNT          907 a         1464 c         1490 g          924 t
ORIGIN      
        1 ggtgcgccag ctcagccctg agcagattca gggcctgcgc cgcagtgccg accattacgt
       61 cgccaatgcg cagcgtttca agaccacgtt gaacaaactt gcctgatacg gaccacttcg
      121 cgtgtctgaa atccacaagt ttttgtttga aggcctgccg gtgcgcggtg tgctggtgcg
      181 tttgaccgat gcctggaccg aggtgctggc ccgccgcgca gccaacacca tgcacggcgc
      241 ttacccgccg ccagtgcaaa acctgctcgg tgaaatgacc gctgcagcgg tgctgatgca
      301 gtccaacatc aagtttgacg gcgcgctggt gctgcagatt tttggcgatg ggccggtcaa
      361 gctggcggtg gtggaagtgc aaccggactt tggcttgcgc gccacggcca cggtgaatgc
      421 cgctatcgat gccactgcca gcctgagcca gatggtcaat gtcggcaatg ccgggcgctg
      481 cgccatcacg cttgatccgc aaaaccgcca gccaggtcag cagccctacc agggtgtggt
      541 accgctgttt ggcgatgcgc gcgaaaaact ggaaaaactc agcgacgtgc tgcagcacta
      601 catgctgcaa agtgaacagt tggacaccac cttggtgctg gctgccaacc agcaagtggc
      661 agccgggctg ctgattcaac gcctgcccat gttgggcagc ggcaacctgg caggtgcgca
      721 gaccgccaat gaggaccaga ttggcatcaa tgagcactac aaccgcattg ccctgctggc
      781 cgctaccctg caacgcgagg aattgctgac gctcgatgcc gaggccatct tgcaccgcct
      841 gttctgggaa gaagacctgc gccggtttga gccgctgcaa ggcgctggcg gaccgcattt
      901 ttcatgcacc tgcagccgcg agcgggtcag caaaatgctg atcgggctgg ggcaggatga
      961 ggcgcaaagc atcttgcaag agcgcggtct gattgaagtg ggctgcgatt tttgcagcca
     1021 gcagtaccgc tttgatgcgg tcgatgtgag ccacatgttt cgccagactg atcaaacgcc
     1081 accggccagc acggcggtgc agtgagtacg cgggaactct tcgtcaagcc aatcagagca
     1141 gttggtcggc gtggttgatc gcggtgtaca gccagatttg agtgagttgc gctcttgcgt
     1201 taggccttca atctgggtgt acagcagggc attggtgcgg gtaagttctt cggtttactt
     1261 cgtcaccctg atgaacgaga acgacgccca gccgaattta cccgagggtg cggcggccga
     1321 tgtgatgcct gattgctagt atttaaggag ctacccacgc acagcagacg ggggccagag
     1381 cacgaaaaca cccacacccg caaatgcaga taaccacctg accctgctgg gcttcgggct
     1441 gattctgacc gaagtggtca cggtcgcgcc attttggcgg cccagcgtat cctggatcag
     1501 cggtgacatt tgaattcaac gggcaacggg ccgattttca tcgccccaat ccaggcaatg
     1561 aatcgctgcg ttaccgggtc agggcagcac gggaattcat tgaaaagatt ggagttttgc
     1621 catgaacagg atgacccata acggctacac agcccagatg gaatttgacg ttgaagacaa
     1681 aatcattgtc ggccgcgtca ttgacatcga cgacatcatc acctttcacg gcacctcggt
     1741 agccgaattc gaggctgttt tcaaagcggc ggtggatggc tatatccagg cttgccaaaa
     1801 gctgggacaa gccgctgaaa agtcggccag tggccgcttg atgcttcgcg taagccccag
     1861 cattcacgct gccgccgtta aagccagcgc ccgcagcggg caaagcctca acaagtgggc
     1921 ggaaaaggtg ttggggcagg ctgcccgcgc tttgccgtaa aaaaaaccgc gcagcccttg
     1981 tggaatttgc tcggcctgct attgaattta tagactatca acggcctgac accctcactt
     2041 gcgcgccgac cgcaggtcca tgcaactgct tcgagccgcc tgcgccgtaa tcctgagccg
     2101 gaccagccgg gatcgatgag ccttcgtcgc gagggcgcgc ctcctacaca aacagtcagc
     2161 gccaatttga atttgtagga gtcgcgccct cgcggcgatt gactttgaat acgtgagttc
     2221 ttcgcatgat atgcaaagac ttgacttgtt aaagactaat taataagcag gcgctgactc
     2281 aggctattga agccatggtt caggttgggc cttggtcact tcccagtgac gctctttgtc
     2341 tgaccgcctt gcccgttcag cgaatttcga tctggaaatg atcggccacg acccggggaa
     2401 tcgctcgcgc cccggcccgt ttttggtcag cgatcgcctc gcgcatgtcc agaatcacct
     2461 gctcggcttc tgcgaaatag ccgtgaccca ggtcgagcag gccaaagccg gtgactgaaa
     2521 tcgtttcaat gccatcgtac tggcgtagcg gcgggacaat gccaacgcgg tcggcctggt
     2581 gtttccagcg cgaaagcgcc accgccttgt cgtcggggga aaccagcagg gtgcggttct
     2641 cggcctggca agggaaggcc tgggttttgt cggcgaagat gcgcgtgtcc tcgtctggcg
     2701 cgcagaagaa cacctggccc aagcgcagcg ccggccgacg cagggccgcc aggcgctcca
     2761 gggcggcaag aaagccgcga ttgcccatgc tgtgaacgaa caggtggatg cgctcggcgc
     2821 ccgagcgctg cgaaagttcg tccagaaagg tggcgatctg gccaacgctg gcgccaatgg
     2881 tggcctcgtc ggccgagtaa tccatcacac cgccctttga gggccaactg tagaaagcca
     2941 tctcgcccgg cagcttgagg tcgtagccga tctgcgccgc ccggactgcc gcctgcgcaa
     3001 agctgacgtt gaagccatgg atcagcacga agacattgcg ctcgcccggc ttccaccagg
     3061 tcgccaactt gtcggccaac agcggccaga aacgttcacg cgccaagcgc acggtggcgc
     3121 gcaatgccag cgtgtcgtcc gcttccagcc gtatccagcg ccgccaccat gcgctgccgg
     3181 tagagccggg ccggtggctc ttggggatga acacgctgca gtgtccgtag ctcagtgtgt
     3241 cggccgactc atcggcgctg aatggctcgg cggggctgcc gccctcgcaa ggcttgcggt
     3301 tggtggcaaa ccagactcgc accaggcggc ggtccgggtc gctccagacc cgctcgtcgg
     3361 cgccgcgttt cagggcgaac agggttggcg cctgggcggc gcgccagaga aagagaaaca
     3421 gcgccgcgca cagttccgcg aacccggctg ccgccaacag gtcgcgcagc ggcaggaact
     3481 cgtcggtggc agccagggcc tcggcgtcgg tgatgccgtc agcggccacc tcggccaaca
     3541 ggggggcaag gtcgatggcg aagtcgtcgg tcagcgtcgc cgtctcggcc cccagggcga
     3601 gggacagggt cagcgcgccc tcggcccgca gggcggaggt gccgaaccag cgacaaaccg
     3661 cagcgcttgc gtccggggtg ctcgcgatgt ggaggtggaa aggtttcatg cggtatcccc
     3721 tgggagccgg cttggccgca ctatcgttgc gctgatcgcg ggcaagcccg tttctacatg
     3781 acggtcggcc atttcagtcc tccggtttcg gctggggtag cagggccgct cggcttgaag
     3841 ggtcgtcgag ggcgtggaga agggtttcga tgatcatgga ggccgtcagg cctatgccgg
     3901 cgcacagtac ctcctcgtcc cgatcgccgg acagaatccg ccgcagcgcc gccgctagtt
     3961 ccgtattctc gccacccgct ttttcgagtt gcgccatgta tttctccgtg gcagtttggg
     4021 cctttgcatc gccaccaatg gctaacacca ccgcagcgat caggggcgag taacgcttca
     4081 tctcatgagc cgtaccagcg tagccgcgtt tcgcctcgcg ggcctggcgg cggtagtcgg
     4141 tggcctcgcg cggtcgtgac tgctgctcgg cgatcttggc gaggaggctg taggtcttcc
     4201 atatctccgc cgcgccgggg tccagggtcg ctttgatcgc cagggcctcc tcggcgagtt
     4261 ggtgggcctc ggctaggcgg ccgggttggt gttgcagcag ttcagcgagg ttgctcagac
     4321 atacagcacc acggtctccg ttaggcgggt tttgcatcgc cgcgatggcc ttgcgatacc
     4381 aagtttcagc cacctccggc ctgccggtgc gctcacagac catggccagt tggttccagg
     4441 tccgggctgc actcgccaaa tcaccccgct tctccttcaa cacggccgac tcgcgatagt
     4501 gccgctcggc ctgctcccac tgcttggcct cctgaaacgc acggccaagc tggtgctggg
     4561 ctaccgcttc catagccggt tcctggaggc gctggaacag cgcgagtgct gcctggtgac
     4621 gctgcacagc ctcgttcagg tcaccctccg ccatggccag ggtaccgagt tgcccctccg
     4681 taacgccctg tccacgcaaa tcatgcaact ccttatcgac ttctagcccc cttaaataaa
     4741 acaggtaatc ccccggcttt gccggggcga cagtaggggt tttac
//
LOCUS       DAOSLL010000365         4780 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15855_length_4780_cov_5.460952, whole genome shotgun sequence.
ACCESSION   DAOSLL010000365 DAOSLL010000000
VERSION     DAOSLL010000365.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4780
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15855_length_4780_cov_5.460952"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="PLB25_14750"
     CDS             <1..192
                     /locus_tag="PLB25_14750"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOE42867.1"
                     /translation="FIACAAGSLGHLVMKALKRDRGIPIWRGEGRGVTGAGGMLDRID
                     SLCFAAPVFFHSVRWFFGL"
     gene            235..783
                     /gene="ruvC"
                     /locus_tag="PLB25_14755"
     CDS             235..783
                     /gene="ruvC"
                     /locus_tag="PLB25_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HOE42868.1"
                     /translation="MRILGIDPGLRTTGFGLVDLAGAELAYVASGTITTTHLDKGLLP
                     ARLKVLFDGIREVVARYQPDAASVEIVFVNVNPQSTLLLGQARGAAITALVSGDLPVA
                     EYTALQMKQAVVGWGRADKKQIQEMVRRLLNLPGLPGPDAADALGLAITHAHAAKGMA
                     RLALADGVIGAGSGKYKAGRSR"
     gene            complement(891..4319)
                     /locus_tag="PLB25_14760"
     CDS             complement(891..4319)
                     /locus_tag="PLB25_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42869.1"
                     /translation="MDVNPNPASTDLAAASDNLNAAINLRLALLGLPLAVGADGASIT
                     GLMAPILARQRELSRRLADRLCPTDTRIQHFLNEYLADVGTAPHLPGRTLVLDQSGLA
                     RGLSLPAHGDFFKSPLLSSYRLRNGVLHNPANDRRTTAGVFHVVEGGLPIPDDKLAVP
                     KLAFSKLLALALMPPAESLQLPFTAGEAAPAKCIVSLLLRPLVVPAVPGFTTEKRMET
                     RFFVPGALVANLDFVESIFGNAGDPYLPENDASLDPDSWTGHTGCVILAPHLTRVPKH
                     LLGLPHWDDATALQRRDGMCYKSETELYNNGSAFKLCARDQRGVIFTIIADNYFGYCK
                     KEVKTQISYSANLFGSAEEEHSGGALVFPSYNEGLEFTDLTAGDDYSLKEVLARDASR
                     FALQPDGHALDLTQPHIILVPEKSTFSLRAKTVSWSMPDGKTSSIRLQAGKTYIGPNG
                     YRMHMEPLSGNPHLWNLVGTSADVTACHKPATVSGGGKSEISKAISDAILVGYVYVAD
                     LEKDLDAVTAILARDFSERFSDPDKLGTDHRLILSRERSMGSVIKLLTPSSRDYNDAY
                     NTWLRAIPQHIKELVFVIKRFHRAEWGDDWRSHYSVDLINGRPGYALKLNGSTLIVNT
                     LRVGFEKDGAWRVFGLRHDFHPAIKVQTEDDITASVVAPVGPDALSRKFVQNCEYRLF
                     QRPDDAVHRGYDRQAEADIATPGTFISNFQPLDGSDAQELMDDSIGFSAFTSPMQQLI
                     QAAAQAPQGSSPKYFVSSAHSRLVDGKPTKNPRYLQLRPDVARPQDTASAELAMRLFR
                     RQSSDEPLITPVHVVAAGRRNNPPEDGIRALCPYSPLHYMELPELFIEFISSMTGKSP
                     STTGAGSEGALTKGPFNALPAIIDLNAAFVSYALTGYDGWVSCAGYVGPNVRVDHDVS
                     LLVPEVFARMSPAERNAQKLIASGALERVADFEHRGQPVLSSRLGYRMTARFASTYFG
                     RLFLHPHAVFTDEMLRPELQDMDVFADGVANIVATHQRVAESYFTDGSISMACPPLKA
                     LLEIMAHGQTESGLGLESPELRALFSRESVLASDWYAARLDAKQRSDEARLTRAVASL
                     YEFLDRPDNAGVATRLGLSARRAKTKTERVRVGSAEYRAGLVGTLGLHPLDELP"
BASE COUNT          914 a         1458 c         1528 g          880 t
ORIGIN      
        1 ttcattgcct gcgccgccgg gtcgctcggg catctggtga tgaaggcact caagcgcgac
       61 cgcggcattc cgatctggcg tggcgagggc cgcggtgtca ccggggctgg cggcatgctc
      121 gaccggattg actcgctgtg ctttgcggca cccgtctttt tccactcggt tagatggttt
      181 tttggcctct agcccttatg gaacatgcgc tgatagctat attaatagta gcaaatgaga
      241 atcctcggca ttgaccctgg actacgcacc accggctttg ggctggtcga tctggcgggg
      301 gccgaactgg cctatgtcgc cagcggcacc atcaccacca cacaccttga caagggcctg
      361 ctaccagcgc gactcaaagt gctgtttgac ggcattcgtg aggtggtggc gcgctaccag
      421 cccgatgccg cctcggtcga gatcgtgttt gtcaacgtca acccgcaatc gaccctgttg
      481 ctcgggcaag cgcgcggggc cgccatcacg gcgctggtgt caggcgacct gccggtggcc
      541 gaatacaccg cgctgcagat gaagcaagcg gtggttggct ggggtcgcgc tgataaaaaa
      601 cagattcagg agatggtgcg ccgcctgctg aacctgcccg gcctgcccgg cccggatgcc
      661 gccgacgcgc tgggtctggc catcacccac gcccatgcgg ccaaaggcat ggcacgactg
      721 gcgctggcag acggggtgat tggtgctggc agtggcaagt acaaggctgg ccgcagccgc
      781 tgaccggcgt gcctgccaag tgccgccacc gcgcagcggc atgccgacaa ggccaccgct
      841 gatcaagctt gcttgccgcg acggccttgg aaaccaattc agtccgggtt tcagggcagt
      901 tcgtcaagcg gatgcaggcc gagggtgccg accagcccgg cccggtactc ggctgagcca
      961 acccgaaccc gttcggtctt ggttttggcg cggcgcgccg acagccccag ccgggtcgcc
     1021 accccggcgt tgtcggggcg gtccagaaac tcgtaaagcg aagccacggc acgggtcagc
     1081 cgggcttcgt cgctgcgctg cttggcatcc aggcgcgcgg cataccagtc gctggccagc
     1141 acgctttcgc ggctgaacag ggcgcgcaac tcaggcgact ccagcccgag gccgctttcg
     1201 gtttgtccat gcgccatgat ttcaagcagg gccttgagcg gcggacaggc catcgagatg
     1261 ctgccatcgg tgaaatagct ctcagccaca cgctggtggg ttgccacaat attggccaca
     1321 ccgtcggcaa aaacgtccat gtcctgcaac tccgggcgca gcatttcatc ggtgaacacc
     1381 gcgtgcgggt gcagaaataa ccggccaaaa taagtgctgg caaagcgcgc cgtcatgcgg
     1441 tagccaagcc ggctcgatag caccggctga ccacggtgct cgaaatctgc cacccgctca
     1501 agcgcgccgc tggcaatcag cttctgggca ttgcgctcgg cgggactcat gcgcgcaaag
     1561 acctcgggca ccagcaagct gacatcgtga tcgacccgca cgttcggccc gacgtaaccg
     1621 gcacaggaaa cccagccgtc gtaaccggtc agcgcataag agacaaaggc ggcgttcagg
     1681 tcgatgatgg ccggcagcgc gttgaacggc cccttggtca gcgcgccttc agaaccggca
     1741 ccggtggtgg atggcgactt gccggtcatc gagctgatga attcgatgaa cagctcgggc
     1801 aactccatgt aatgcagcgg gctgtagggg caaagtgccc gaatgccgtc ttcgggcgga
     1861 ttgttgcggc gaccggcggc caccacatgc accggcgtga tcagcggctc gtcgcttgac
     1921 tggcggcgaa acaggcgcat cgccaattcg gccgaggcgg tgtcttgcgg gcgcgccacg
     1981 tccgggcgca actggaggta acgcgggttt ttggtgggtt tgccgtccac cagacgcgaa
     2041 tgcgccgaag agacgaaata ctttggcgaa gagccttgcg gggcctgggc cgccgcctgg
     2101 atcaactgct gcatcgggct ggtgaaggcg ctaaagccaa tcgagtcgtc catcagttcc
     2161 tgcgcgtctg aaccgtcaag cggctgaaag ttggaaatga aagtgcccgg cgtggcgatg
     2221 tctgcctcgg cctggcggtc gtaaccccgg tgcaccgcat cgtcgggccg ttgaaacagc
     2281 cggtactcgc agttttgcac aaacttgcgc gacaaggcat ccggcccaac cggtgccacc
     2341 acgctggcgg tgatgtcgtc ttcagtttgc accttgatcg ccgggtgaaa gtcatgccgc
     2401 aagccaaaca cccgccaggc accgtctttt tcgaaaccga cccgcagcgt gttgacaatc
     2461 agcgtgctgc cgttgagctt gagcgcatag cccggtcggc cattgatcag gtcaaccgaa
     2521 taatggctgc gccagtcatc gccccattcg gcgcggtgga agcgcttgat gacaaacacc
     2581 agttccttga tgtgctgcgg aatcgcccgc agccaggtgt tgtaggcatc gttgtagtcg
     2641 cggctcgacg gggtcagcag cttgatgacg ctgcccatgg agcgctcgcg gctcaaaatc
     2701 agccgatggt cggtgcccag tttgtcgggg tcgctgaaac gttcggaaaa atcgcgcgcc
     2761 aggatggccg tcaccgcgtc caggtctttt tcaaggtcag ccacgtacac ataaccgacc
     2821 agaatcgcgt ccgagatcgc cttggaaatt tcggacttgc caccgccaga aacagtggct
     2881 ggcttgtggc aggccgtcac atcggccgag gtgccaacca ggttccaaag gtgcggattg
     2941 ccgctcagtg gctccatgtg catgcggtag ccattcgggc caatgtaggt tttgccggcc
     3001 tgcagccgga tcgaagatgt cttgccgtct ggcattgacc acgaaaccgt cttggcgcgc
     3061 aggctgaagg tggacttctc gggcaccaga atgatgtggg gctgcgtcag gtccagcgca
     3121 tggccatcgg gctgcaaggc aaagcgactg gcatcgcgcg ccagcacctc tttgagcgaa
     3181 tagtcatcgc cagccgtcag gtcggtaaat tccagccctt cgttgtaact tggaaacacc
     3241 agtgcaccac ctgaatgctc ttcttcggca gaaccaaaca ggttggccga atagctgatc
     3301 tgggttttga cttccttttt gcagtagcca aaatagttgt cggcgatgat ggtaaatatc
     3361 accccgcgct ggtcgcgcgc gcacaacttg aaggcactgc cgttgttgta aagctcggtt
     3421 tcgctcttgt agcacatgcc gtcgcgccgt tgcagcgcgg tcgcgtcgtc ccagtgcggc
     3481 aggcccagca ggtgttttgg cacccgcgtc aggtgcggcg ccagaatcac gcagccggtg
     3541 tggccggtcc aactgtccgg atcaagcgac gcatcgtttt ctggcaggta ggggtcgcca
     3601 gcgttgccaa aaatgctctc gacaaaatcc aggttcgcga ccagcgcgcc gggcacaaaa
     3661 aagcgggttt ccatccgctt ttcggtggta aagccgggca ccgccggcac caccagcggg
     3721 cgcagcaaca aggacacaat gcatttggcc ggggccgctt cgcccgccgt aaagggcagt
     3781 tgcagcgact cggctggcgg catcaaggcc agcgccagca gcttggagaa agccagcttg
     3841 ggcacagcca gcttgtcgtc aggaatcggc aaaccgcctt cgacaacgtg aaaaaccccg
     3901 gccgtggtgc gccggtcatt ggccgggttg tgcagcacgc cattgcgcag ccggtagctg
     3961 ctgagcagcg gcgacttgaa aaagtcgccg tgcgctggca gcgacaggcc acgcgccaga
     4021 ccggactggt ccagcaccag cgtgcgcccc ggcaagtgcg gtgcagtgcc aacgtcggcc
     4081 agatactcat tgagaaagtg ctgaatgcgc gtgtcggtcg ggcacagccg gtcggccagg
     4141 cgacggctca gttcgcgctg gcgcgccagg atgggtgcca tcagcccggt gatggatgcc
     4201 ccatcggcac ccactgccag tggcaagccc agcaacgcca gacgcaggtt gatggcggca
     4261 ttcaggttgt cgctggccgc agcgaggtcg gtggaggcgg gatttgggtt gacatccatt
     4321 tgggtactca ggttagtggt ctgggaaaac cagtatagat tgacagggcc aaaacggcgg
     4381 tttgaaggac ttggaccggt gccgtcgagt tggcagacgt gggtgggcga agcgccgcac
     4441 cgctcgctgc agcggcttgc tgcgccgcac cagccgcttt tcatcactgc gtgtgcagcc
     4501 atgctgttgc gcacgccgcg gtctgtgcgg gccatgtcca ggcgggccac caaggcgcgg
     4561 ccagttcaac atcgggcgaa agcctcccca ctttgacgaa aactcgctac accggtgttg
     4621 cccgtagagg cagctttgga accacatggc aactgaactc ggcccaaggg tagtcctggc
     4681 gcacctaagc cagcgtcaag ccgcgccttc gctatagtca gatttgatag ctgtcagcgc
     4741 aatatgctaa agggctgcag ggcaatttga tgcccaagcc
//
LOCUS       DAOSLL010000366         4758 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15941_length_4758_cov_4.547523, whole genome shotgun sequence.
ACCESSION   DAOSLL010000366 DAOSLL010000000
VERSION     DAOSLL010000366.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15941_length_4758_cov_4.547523"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="PLB25_14765"
     CDS             complement(<1..730)
                     /locus_tag="PLB25_14765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOE42870.1"
                     /translation="MPASPSKPKTTASPAALALAPTDESEQVRSGTFVSFPDSPFELY
                     QPYPPAGDQPEAIRQLVDGINDGEVFQTLLGVTGSGKTFTMANVIARLGRPAIVFAPN
                     KTLAAQLYSEFREFFPKNAVEYFVSYYDYYQPEAYVPQRDLFIEKDSAINEHIEQMRL
                     SCTKSVLERRDVVIVATVSAIYGIGQPEAYHKMVMTLRAGDKMGQRDMIAQLVRMQYE
                     RNDVDFSRGAFRVRGDTIDVFPAEH"
     gene            complement(799..1305)
                     /locus_tag="PLB25_14770"
     CDS             complement(799..1305)
                     /locus_tag="PLB25_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOE42871.1"
                     /translation="MKQLERLLFLQGNRCFFCAGEVPVGEASVEHLLATSNGGPKEDA
                     NCVVCCKAVNAALGNLSIKEKFQAVLNQRARFVCPRPSGSAIAEESRAVTHEEVAGKI
                     DLVVAYLQKHSQSRPGRVAALKNTMNSVFQKTLTEPELDVLVSNLQSLGHIIVEGTKV
                     TYALPIGS"
     gene            complement(1479..2480)
                     /locus_tag="PLB25_14775"
     CDS             complement(1479..2480)
                     /locus_tag="PLB25_14775"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016452944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HOE42872.1"
                     /translation="MKKTYLTRYLVEQQRLNQHISPELRLMLEVVARACKRISQAVNK
                     GALGEVMGSADSSNVQGEVQKKLDIIANEVLIEANEWGGHLAAMASEEMEGIHVVPHR
                     YPQGEYLLLFDPLDGSSNIDVNVSIGTIFSVLVKPEGAGVSEQDFLQKGTKQVAAGYC
                     VYGPQTTLVMTVGDGVMVFTLDREQGSFVLTQEHLKIPEDTKEFAVNMSNQRHWAAPV
                     KRYIDECLQGRDGPRGKDFNMRWVGSMVADVHRILSRGGVFLYPWDNREPEKPGKLRL
                     MYEANPMSWLVEQAGGAATDGKRRILDIEPTSLHARVSVFMGSKNEVDRVTGYHAEA"
     gene            complement(2500..>4758)
                     /gene="pepN"
                     /locus_tag="PLB25_14780"
     CDS             complement(2500..>4758)
                     /gene="pepN"
                     /locus_tag="PLB25_14780"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HOE42873.1"
                     /translation="LRADRAKYPVLLSNGNLVAQGDLEGAGNEGRHFATWVDPHRKPC
                     YLFALVAGKLVAREQRIVSRAGRDHLLQIYVRAGDMDKTEHAMNSLINSVIWDEVRFG
                     LPLDLERFMIVATSDFNMGAMENKGLNIFNTKFVLANQATATDLDFANIESVVGHEYF
                     HNWTGNRITCRDWFQLSLKEGLTVFRDQEFSMDLCADASARAVKRIEDVRVLRTAQFP
                     EDAGPMAHPVRPDSYIEINNFYTVTIYEKGAEVVRMMQTLVGRSGFAGGMTLYFARHD
                     GQAVTCDDFAQSIADANPDSALAKLLPQFKRWYSQAGTPRVNAACQYDAQAKTCTLSF
                     VQSCPATPGQDAKEPFVIPISLGLVAADGAAVPLQIGGRLVGNDGSHLFVMTQATESV
                     TFENVPEPPVPSILRGLSAPVILEFDYTDSQLLTLLQCDPDPFNRWEAAQRLGLRCAI
                     DSIAASADVSSARPLNDAFIQAMRSVLRSPTLDAAFKDLVLTLPSETYIAEQLALVDP
                     QRIHAVREAMREQLAQALLADWQWAFEAHQHNGGYRPDTLSSGRRALAGLSLSYLCLA
                     ARSSGDTVWPGKAYQRFKDADNMTDRFGALSALVHSGHPLAAKALARFHRLFKGEELV
                     LDKWFALQAGASDRGGLVLPAVRLLMQHADFNLRNPNRARSLIFSYCSANPGAFHRAD
                     AAGYVYWSEQVLALDAINPQLAARLARALDRWKKLAEPYRGAACEALTRVAAKTDLSN
                     DVREVVSRALAD"
BASE COUNT          910 a         1439 c         1427 g          982 t
ORIGIN      
        1 aatgttcggc cggaaaaacg tcgatggtgt cgccacgcac ccgaaacgcc ccgcgtgaaa
       61 aatcaacgtc gttgcgctca tactgcatgc gcaccaactg ggcaatcatg tcgcgctggc
      121 ccattttgtc gcccgcgcgc agcgtcatca ccatcttgtg gtacgcctcg ggctggccaa
      181 tgccgtaaat cgccgacact gtggccacga tcaccacatc gcgccgctcc agcacgctct
      241 tggtgcaaga caggcgcatc tgctcgatgt gttcgttgat ggcagagtct ttttcaatga
      301 acaggtcgcg ctgcggcaca taggcttcag gctggtaata gtcgtagtag ctgacgaaat
      361 actccactgc gtttttcggg aaaaactcgc gaaactcgct gtagagctgc gctgccagcg
      421 tcttgttggg ggcaaaaaca atcgctggcc gacccagccg ggcaatcaca ttggccatgg
      481 tgaaggtctt gcccgagccg gtgacaccca gcagggtctg aaacacctca ccgtcattga
      541 tgccatcgac cagttgccgg atcgcctcgg gttgatcccc tgcgggcggg tacggctggt
      601 aaagttcaaa gggagaatcc gggaagctga caaaggtgcc agagcggacc tgttcggatt
      661 cgtcagtggg tgccaatgcc agcgcagccg ggctggcagt ggtttttggt ttggaagggg
      721 atgcaggcat gcggcggtca atgttcgagg tgaggcacac attgcagctt gctgcaatgt
      781 gtcgcctcga ctgaggggct aggagccaat gggtagcgca taggtgacct ttgtgccttc
      841 gacgatgatg tggcccaaag attgcagatt ggacactagg acgtcgagtt caggctctgt
      901 taaggtcttc tgaaatacag agttcatggt gttcttcaag gccgctacgc gcccgggccg
      961 ggattggctg tgcttctgaa ggtatgccac cacaagatca atcttccccg caacctcctc
     1021 gtgagtgacg gcgcggctct cttctgcaat tgcgctaccg cttggtcttg ggcagacgaa
     1081 ccgtgctcgc tggtttaata ccgcctggaa tttctccttg attgagaggt tgccgagagc
     1141 ggcattgaca gccttgcaac agacaacaca gttcgcgtcc tccttggggc caccatttga
     1201 tgtggccaat agatgctcaa cgctagcttc accgaccgga acctccccgg cacagaagaa
     1261 gcacctgttc ccttgaagga agaggagtcg ttcaagttgc ttcattcagg cacttcactc
     1321 aatatataca ggcctgcatg gaagcggctt ggcatctggc atccatgccc cggttgaagc
     1381 tgcgcgctgc ccctcctgct ggcccatgat tggggccacc gaccgcgcca aaccggcctg
     1441 acgagcaccg gtttagcgcc agccggacag acctggcttc aggcctcggc gtgataaccg
     1501 gtaacgcggt caacttcgtt tttcgaaccc atgaagacgc tgacccgcgc atgcaggctg
     1561 gtcggctcga tgtcaagaat gcgccgcttg ccgtcggtgg cggcgccacc ggcttgctct
     1621 accagccagc tcatcgggtt ggcttcgtac atcaggcgca gcttgccggg tttttcgggt
     1681 tcgcggttgt cccaggggta caggaaaacg ccgccacggc tcaaaatgcg gtgcacatcg
     1741 gccaccatgc tgccgaccca gcgcatgttg aagtctttgc cgcgcgggcc gtcgcgaccc
     1801 tgcaggcatt cgtcgatgta acgcttgacc ggtgccgccc aatggcgctg gttgctcatg
     1861 ttgacggcaa attccttggt gtcctcggga atcttgaggt gctcttgcgt cagcacaaac
     1921 gagccctgct cgcggtccag ggtgaacacc atgacgccat cgccgaccgt catcaccaga
     1981 gtggtctggg gaccatagac gcagtagccg gccgccacct gtttggtgcc tttttgcaaa
     2041 aagtcctgct ctgacacgcc ggcaccttcg ggcttgacca gcacgctgaa aatggtgccg
     2101 atgctgacat tcacgtcaat gttgctggag ccatcaagcg gatcaaacag cagcaggtat
     2161 tcgccctgcg ggtagcgatg gggcaccacg tggatgcctt ccatttcctc gctggccatg
     2221 gccgccaggt ggccacccca ttcgttggct tcaatcaaca cttcattggc gatgatgtcg
     2281 agctttttct gcacttcgcc ctgcacgttg gaactgtcgg cgctgcccat cacctcgcct
     2341 agcgccccct tgttaaccgc ctggctgatg cgcttgcagg cgcgggccac cacttcaagc
     2401 atgaggcgca gctcaggcga gatgtgctgg ttcaagcgtt gctgctcgac caggtagcgg
     2461 gtgagataag tttttttcat agtgaactcc tgtggttggt caatcggcca gtgcgcggct
     2521 gaccacctcg cgcacatcgt tgctcaaatc tgtcttggcg gcgactcggg tcagcgcctc
     2581 gcaggcggca ccccgatagg gctcggcgag cttcttccag cggtccaggg cgcgcgccag
     2641 gcgcgcggcc agttgcgggt tgatggcatc aagcgccagt acctgctcgc tccagtacac
     2701 ataacccgcc gcatcggcgc ggtggaaggc acccggattg gcgctgcaat agctaaatat
     2761 caggctgcgt gcacggttcg ggttgcgcaa attgaagtcg gcatgctgca tcagcaggcg
     2821 taccgctggc agcaccaggc caccccggtc gctcgccccc gcctgcagtg caaaccattt
     2881 gtcgagcacc agttcttcgc ctttgaacag gcggtgaaaa cgtgccagcg ccttggctgc
     2941 aagcgggtgc ccgctgtgca ccagcgcgct cagcgcaccg aagcgatcag tcatgttgtc
     3001 ggcatccttg aagcgctggt acgccttgcc cggccaaacg gtgtcgccgc tgctgcgggc
     3061 agccagacac aaatagctca acgacagccc ggccagcgcc cggcggccgc tggaaagcgt
     3121 gtccgggcgg tagccgccgt tgtgctggtg cgcctcaaac gcccactgcc agtcggccag
     3181 cagggcttgg gcaagctgtt cacgcatggc ctcacgcacc gcatggatgc gctgcgggtc
     3241 aaccagcgcc agttgctcgg caatgtaggt ttcagacggc agcgtcagca ccaggtcttt
     3301 gaaggcagca tccagcgtgg ggctgcgcaa gacgctgcgc atggcttgaa taaatgcatc
     3361 attcaggggt ctagcgctac tcacatctgc gctggcagct attgaatcaa tagcacatcg
     3421 caaacccagc cgctgcgccg cttcccagcg gttgaacgga tcggggtcgc actgaagcag
     3481 ggtcagcaac tggctgtcgg tgtagtcaaa ttccagaatc accggtgcgc tgaggcctcg
     3541 caagatagac ggtaccggcg gctctggcac gttctcgaaa gtgaccgact cggtggcctg
     3601 tgtcatgaca aacaggtggc tgccgtcgtt gccgaccagc ctgccgccaa tttgcagcgg
     3661 cacggcggca ccatcggcgg ccaccaggcc caggctgatc gggatgacaa aaggttcttt
     3721 ggcatcttgc cctggcgtgg ccggacaact ttggacaaaa ctcagcgtgc aggttttggc
     3781 ctgtgcatcg tattggcagg cggcattgac acggggtgtg ccggcctggc tgtaccaacg
     3841 cttgaactgc ggcagcagct tagccagcgc cgagtccgga ttggcatcgg caatcgactg
     3901 ggcgaagtca tcgcaggtca ccgcctggcc gtcgtggcgg gcaaagtaca gcgtcatgcc
     3961 accggcaaaa cctgatctgc cgaccagggt ctgcatcatg cgcaccactt cagccccttt
     4021 ttcatagatg gtgacggtgt agaaattgtt gatctcgata tagctgtcgg gccgcaccgg
     4081 atgcgccatc gggccggcat cttcagggaa ctgggcggtg cgcagcaccc gcacatcttc
     4141 aatgcgcttg accgcgcggg ccgacgcgtc ggcacacagg tccatgctga attcctggtc
     4201 gcgaaacacc gtcagccctt ctttcaggct caactgaaac cagtcgcggc aggtgatgcg
     4261 gttgccggtc cagttgtgaa agtactcgtg accgaccacg ctctcgatgt tggcaaagtc
     4321 caggtcggtg gcggtggcct ggttggccag cacaaacttg gtgttgaaga tgttcaggcc
     4381 cttgttttcc atcgccccca tgttgaagtc gctggtggcc acaatcatga agcgctccag
     4441 gtccagcggc agaccgaagc gcacttcgtc ccagatcact gaattgatca gcgaattcat
     4501 cgcgtgttcg gttttgtcca tgtcaccggc tcgcacataa atctgcagca aatggtcccg
     4561 cccagcgcgt gacacgatgc gttgctcacg cgccaccagt ttgcctgcca ccaaggcaaa
     4621 caggtagcag ggtttgcggt gcggatcgac ccaggtggcg aagtgtcggc cctcgttgcc
     4681 tgcgccttcc agatcgcctt gcgccaccag gttgccgttg gacagcagca ccgggtattt
     4741 ggcccggtcg gcacgcag
//
LOCUS       DAOSLL010000367         4755 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_15956_length_4755_cov_4.797872, whole genome shotgun sequence.
ACCESSION   DAOSLL010000367 DAOSLL010000000
VERSION     DAOSLL010000367.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4755)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15956_length_4755_cov_4.797872"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2938
                     /gene="recC"
                     /locus_tag="PLB25_14785"
     CDS             <1..2938
                     /gene="recC"
                     /locus_tag="PLB25_14785"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HOE42874.1"
                     /translation="QTLDAQQQQSIRPLVAQRVLARLASGEPLASRVAQRVIVFGMSQ
                     IPWTTLQALAALAAHRQVMLAIPNPCRFYWGDIMDGRELLNSQRRRQPLRQGRELSAL
                     PLEDMHAHAHPLLAAWGRQGRDFIRQLDVFDDAEHCRLKFGLARLDLFDETEEDEQMP
                     LLRRVQNRIRDLVPLADHTKLPLAAHDDSIVFHSAHSKVRELEILHDQLLQLLAQPAG
                     EAALNPRDVIVMVPDITQMAPAIRAVFGQYKRQDARFIPFDIADMSARTSSPVIGALD
                     WLLRLPQQRCCMSELVDLLEVPAVALRFGIDQESLPRLTRWMVGAGIRWGLNLEHRAD
                     LGLLACGDQNSAWFGLRRMLLGYASGVVPADDPLADFSDIEPYGEVAGLEAELAGSLA
                     HLLQALVDWWTVANTPAEPASWAQRGRTLLAAMFRCESPTDKQALGALEDGLIAWQTA
                     CEEAGFVQAVPLIVARSAWLEAIDVPRLNQRFQAGGVTFCTLMPMRAIPFEVICLLGM
                     NDGDYPRRSQRSDFDLMGLPGTSRPGDRSRRDDDRQLMLEALLSARRQLYISWCGHSV
                     RDNSEQPPSVLVSQLRDYLSAGWGQQVVSGRTTVHPLQPFSRRYFEAGSPLLTYAREW
                     RAAHESSAVATDDPLGTALATQLGTSFCADPNLPVTLSQLTSFLRNPVKAFFRQRLQV
                     VFDEDLQDRADEECFALDGLQEYGLIQDVLTSASARTDAVQGAACVTSALTRLHNAGE
                     LPLKGLGDLKQQALHAVLTTMLQAWRTEQARFPEPAERQSVRLQQGEVVLEDWIDHLR
                     RGEALPAPATSQPHSVSTAWLELQPGKLLEKGAKPVARADKLLGPWVRSLALAASGLN
                     ACGVLVGRDGLVDISPMPKDKAQATLRMLLSLWLLGMQAPLPLPPKTALAQLKDKGPI
                     AQYEGSFMQTGENDEPCLARMFPDFEALSAEGRFQSLARQVYAPLLDWANQHVTARFH
                     RTEAAL"
     gene            3045..>4755
                     /locus_tag="PLB25_14790"
     CDS             3045..>4755
                     /locus_tag="PLB25_14790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016453545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HOE42875.1"
                     /translation="MTSTTPEPANKVLHPATFDLWGSRLIEASAGTGKTWTIAALYLR
                     LVLGHGGEHAFERPLRPAEILVMTFTRAATRELSDRIRERLLRAAQCFRGERQPEPDD
                     TFLTTLLADYAPGPQRNRAAWVLAMAAESMDDAAVHTIDAWCQRMLKEHAFDSGCLFD
                     EELVADEEAMRIEATHDYWRQACYPLAAEPLAQVLAIWRDVDQLSADLRGLLNQDIAP
                     EAGDGTLAEVLAQATTQRATALAQIKEGWAVRAQTMQDWLDAQTASKNSGWNGHKLSP
                     KNYTGWLNTLKSWANGAIEDDKPDLKTGWERFTPDGLLLARKPGAPSIALPPEFAQFA
                     ALEVALDKLPDIRVAVRLHAAATVAQRLLQLKRQSGSFGFADMLRRLNSALGGQNGTR
                     LRERILAQYPVILVDEFQDTSPLQYQIFDQIYQTQTNDRHSALVLIGDPKQSIYGFRG
                     ADIYSYMQARRATSGRHYVLETNFRATQPLVDVVNHWFAHAEATRTEGAFMYRQGDDN
                     PLPFVNVVANGRKEQLVNANGPVPAMTLVHDLELINADGMRKRFAARCAEQIVRWLND
                     RQAG"
BASE COUNT          870 a         1520 c         1493 g          872 t
ORIGIN      
        1 gcaaacgctt gacgcccagc aacagcaaag catccgcccg cttgttgctc agcgggtgtt
       61 ggcgcgcctg gcatcgggcg agcccctggc cagccgggtg gcgcaacggg tgatcgtgtt
      121 tggcatgagc cagatcccct ggaccaccct gcaggccttg gcggcgctgg cagcccaccg
      181 ccaggtcatg ctggcgatcc ccaacccctg tcgcttctat tggggcgaca tcatggatgg
      241 ccgtgaattg ctcaattcgc agcgcaggcg ccagccgtta cgccagggcc gggagctgtc
      301 ggccttgccg ctggaagaca tgcatgccca tgcccatccg ctgctggctg cctggggccg
      361 gcaggggcgc gacttcatcc gtcaactcga tgtctttgac gatgccgagc attgccgcct
      421 gaaattcggt ctggcccgcc ttgatttgtt tgacgagact gaggaagatg agcagatgcc
      481 cttgctcagg cgggtgcaaa accgcattcg tgacctggtg ccgttggcgg accacaccaa
      541 actgccgttg gcggcgcatg acgattccat cgttttccac agcgcccaca gcaaggtgcg
      601 tgaactcgaa atcctgcatg accaactgtt gcagttgctg gcccagcctg ccggtgaggc
      661 agcgcttaac ccgcgcgatg tgattgtgat ggtgcccgac atcacgcaaa tggccccggc
      721 catccgggcc gtttttggtc aatacaagcg ccaggacgcg cgtttcatcc cgtttgacat
      781 tgccgacatg agcgccagaa ccagcagccc ggtgattggc gcgctggact ggttgctgcg
      841 cctgccgcag cagcgctgct gcatgagcga actggtggat ttgctggaag tacccgccgt
      901 ggcgctgcgt tttggcattg atcaggaatc cctgccccga ctcacccgct ggatggtggg
      961 ggccggcatt cgctggggct tgaaccttga gcatcgcgcc gatctgggtc tgctggcctg
     1021 tggcgatcaa aacagtgcct ggtttggcct gcgccgcatg ctgctgggct atgccagtgg
     1081 cgtggtgccg gccgacgatc cgctagccga cttcagcgac attgagccct acggcgaggt
     1141 ggcgggtctg gaagccgagc tggccgggtc gctggcccac ctgttgcagg cgctggttga
     1201 ctggtggacc gtggccaaca cgccagcaga gcccgcaagc tgggcgcagc gcggccgcac
     1261 cttgctcgcc gcgatgttca ggtgcgagtc gcccaccgac aaacaggcac ttggcgcgct
     1321 tgaagacggg ctgattgcct ggcaaacggc ctgtgaagaa gccggttttg tccaggcagt
     1381 gccgctgatc gtggcccgct cggcctggct ggaggcgatc gacgtgccca ggctgaacca
     1441 gcgctttcag gccggcggcg tcactttttg cactttgatg ccgatgcggg ccattccgtt
     1501 tgaagtgatt tgcctgctcg gcatgaacga cggcgactac cccaggcgca gccagcgcag
     1561 cgactttgac ctgatggggt tgccaggcac ctcgcgccct ggcgaccgct cgcgccgcga
     1621 tgatgaccgt caactgatgc tggaggcctt gctgtcggcc cggcgccagc tctatatcag
     1681 ttggtgcggc cacagcgtgc gcgacaacag cgagcagccg ccctcggtgc tggtgtcgca
     1741 attgcgcgac tacttgagcg ccggctgggg ccagcaggtg gtgtccgggc gcaccaccgt
     1801 gcaccccttg caaccgttta gtcggcgcta ctttgaagct ggcagcccct tgctgaccta
     1861 tgcccgcgag tggcgtgcgg cccatgagtc cagcgcagtc gccaccgatg accccctggg
     1921 cacggctttg gcgacccagc ttggcaccag tttttgcgcc gaccccaatc tgccagtgac
     1981 cctgagccag ttgaccagtt ttctgcgcaa tccggtgaaa gcattttttc ggcagcggct
     2041 gcaagtcgtc tttgatgaag accttcagga ccgggccgac gaagagtgtt ttgcccttga
     2101 tggcctgcag gaatacgggc tgatccagga tgtgctgacc agcgcaagcg cccggaccga
     2161 cgcggtccaa ggtgcggcct gcgtgaccag cgcgttgaca cgcctgcaca acgctggtga
     2221 gttgccactc aaaggcctgg gcgacctcaa acaacaggcc ctgcatgcgg tgctgaccac
     2281 catgctgcag gcctggcgca ctgagcaagc ccgctttcct gaaccggcag agcgtcagtc
     2341 tgttcgcctg caacagggcg aggtggtgct ggaagactgg attgatcacc tgcgccgggg
     2401 tgaagccttg ccagcgcctg ctaccagcca gccgcacagc gtcagcaccg cctggctgga
     2461 actgcagccg ggcaaactgc ttgaaaaagg ggccaagcca gtggccagag ccgacaagct
     2521 gctcggccct tgggtgcgca gcctggcgct tgccgccagt ggcttgaacg cctgcggcgt
     2581 gctggttggg cgcgacgggc tggttgacat ctcgccgatg cccaaggaca aagcgcaggc
     2641 cacactgcgc atgctgctgt cactttggct gctaggcatg caggcgccat tgccgctgcc
     2701 gcccaaaacg gcgctggccc agttgaaaga caaaggcccg attgcgcaat acgaggggag
     2761 ctttatgcaa actggtgaaa acgatgagcc ctgcctggcc cgcatgttcc cggactttga
     2821 ggctttgagt gctgaaggcc gcttccagag tctggcgcgg caggtttatg cacccttgct
     2881 ggactgggca aatcagcatg tgacagcccg ctttcaccgc accgaggcgg cgctttgatg
     2941 cgctgacaat ttatgatatt ttggcctcta gcgcttattc atctagcact accagctatc
     3001 aactatgtag cgtttgagtt acgtttttac acagataagc gtccatgaca agtaccaccc
     3061 ccgaaccagc gaacaaagtc ctgcatcccg ccacctttga cctgtggggc agccggctga
     3121 tcgaagccag cgctggcacc ggcaaaacct ggaccatcgc cgccctgtac ctgcgtctgg
     3181 tgctcggcca cggcggcgaa catgcttttg agcggccgct gcgcccggct gaaatcctgg
     3241 tgatgacctt taccagggct gccacgcgcg aactgtcaga ccgcattcgc gaacgcctgc
     3301 tccgcgccgc ccagtgtttt cggggggagc gacagcccga gccggatgac actttcctga
     3361 ccaccttact ggccgactat gcgccggggc cgcagcgcaa ccgtgccgcc tgggtcttgg
     3421 cgatggccgc agaaagcatg gacgatgccg ccgtgcacac catcgatgcc tggtgccaac
     3481 gcatgctcaa ggaacacgcc tttgacagcg gttgcctgtt tgacgaagaa ctggtggccg
     3541 atgaagaagc catgcgcatc gaagcgactc acgattactg gcggcaggct tgttacccgc
     3601 tggctgccga gccgctggcg caggtgctgg caatctggcg tgacgtggac cagctcagcg
     3661 ctgacctgcg cgggctgctg aaccaggaca ttgcgccaga agcaggcgat ggaacgctgg
     3721 ccgaggtgct ggcccaggcc accacgcaac gcgcgacggc gctggcgcaa atcaaggaag
     3781 gctgggcggt acgggcgcaa accatgcagg actggctgga cgcgcaaacc gccagcaaga
     3841 acagcggctg gaacggtcac aaactcagcc caaagaacta caccggctgg ctcaacaccc
     3901 tgaaaagctg ggccaacgga gcgattgaag acgacaagcc tgacctgaag accggctggg
     3961 agcgcttcac cccggacgga ctgctgctgg cccgcaagcc cggtgcgccg tcaatcgccc
     4021 tgccgcctga gtttgcccag tttgccgcac tcgaagtggc gcttgacaaa ctgcctgaca
     4081 tccgcgtggc ggtgcgactg cacgctgccg ccacggtagc gcagcggctg ctgcagctca
     4141 aacgccaaag cggcagtttc ggctttgccg acatgctgcg ccgcctcaac agcgccctgg
     4201 gcggccagaa tggcacgcgc ctgcgcgaac gcatcctggc ccagtacccg gtgatcctgg
     4261 ttgacgagtt tcaggacacc tcgccgctgc agtaccagat ttttgaccag atctaccaga
     4321 cccagaccaa tgaccgccac agcgcgctgg tgctcattgg tgacccgaaa cagtcgatct
     4381 acggttttcg gggtgccgac atctacagct acatgcaggc gcgccgcgcc accagcggcc
     4441 gccactatgt gctggagaca aactttcgcg ccacccagcc cctggtcgat gtggtcaacc
     4501 actggtttgc ccatgccgag gcaacgcgca ctgaaggggc cttcatgtac cgccagggtg
     4561 acgacaatcc cctgccgttc gtcaacgtcg tggccaatgg ccgcaaagag cagttggtca
     4621 acgcgaatgg cccggtgcct gccatgaccc tggtgcacga tctggagctg atcaatgccg
     4681 acggcatgcg caagcgcttt gccgcgcgct gcgccgagca gattgtgcgc tggctcaatg
     4741 accgccaggc cggtt
//
LOCUS       DAOSLL010000368         4743 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16003_length_4743_cov_4.410836, whole genome shotgun sequence.
ACCESSION   DAOSLL010000368 DAOSLL010000000
VERSION     DAOSLL010000368.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16003_length_4743_cov_4.410836"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="PLB25_14795"
     CDS             <1..196
                     /locus_tag="PLB25_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HOE42876.1"
                     /translation="QDSVGGITPNTLNTRIKELREADLITHGNKGYLVTAAGADLLKR
                     MGDLSAFATKWASGQAKKAV"
     gene            242..592
                     /locus_tag="PLB25_14800"
     CDS             242..592
                     /locus_tag="PLB25_14800"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HOE42877.1"
                     /translation="MTTTPSGLEYDDNTTGTGDLAAKGHEVTVHYTGWLYQDGVQGPK
                     FDSSKDRQSPFVFTLGAGMVIKGWDEGVAGMCVGGVRTLIIPSTLGYGARGAGGVIPP
                     NATLKFEVELLGVR"
     gene            778..1365
                     /gene="grpE"
                     /locus_tag="PLB25_14805"
     CDS             778..1365
                     /gene="grpE"
                     /locus_tag="PLB25_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HOE42878.1"
                     /translation="MIDPTPHTTGADNSAAHTETAAPDQQDIQNITAAMANYEAEALN
                     KCQADLAQLQAKSAELADQFLRAKADAENARRRAEDDVSKARKFAIEGFADSLLPVID
                     SLEAGLGVKEGALEAIREGAEATLRQLLAALERNKVVVINPAPAAKFDPHLHQAISVV
                     PSDQEPNTVVMVLQKGYLISERILRPALVTVSGPK"
     gene            1439..3382
                     /gene="dnaK"
                     /locus_tag="PLB25_14810"
     CDS             1439..3382
                     /gene="dnaK"
                     /locus_tag="PLB25_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HOE42879.1"
                     /translation="MGRIIGIDLGTTNSCVAIMEGNSPKVIENSEGARTTPSIIAYQE
                     DGEVLVGASAKRQAVTNSKNTLYAVKRLIGRKFTEKEVQKDINLMPYKIAAADNGDAW
                     IEVRGTRLAPQQISADVLRKMKKTAEDYLGEAVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAFGLDKNEKGDRKIAVYDLGGGTFDISIIEIADVDGEKQFE
                     VLSTNGDTFLGGEDFDQRIIDFIIAEFKKEQGVDLSKDVLALQRLKEAAEKAKIELSS
                     SAQTDINLPYVTADASGPKHLSIKLTRSKLESLVEELIERTMAPCRTAIKDAGVSAAD
                     IHDVILVGGMTRMPKVQEKVKELFGKEPRKDVNPDEAVAVGAAIQGQVLSGDRKDVLL
                     LDVTPLSLGIETLGGVMTKMITKNTTIPTKFAQTFSTADDNQPAVTIKVFQGEREIAA
                     GNKLLGEFNLEGIPPAARGLPQIEVSFDIDANGILHVGAKDKGTGKENKITIKANSGL
                     SEAEIQQMVKDAELNAADDKKKLELVQARNQAEASLHSVKKSLTEYGDKLDAGEKEKI
                     EAAVKDLEAVLKGEDKAAIDDKSAALMTVSQKLGEKMYADMQAKQAAEGNSGADGAQA
                     SPQSGTAQDDNVVDAEVKEVKKP"
     gene            3485..4621
                     /gene="dnaJ"
                     /locus_tag="PLB25_14815"
     CDS             3485..4621
                     /gene="dnaJ"
                     /locus_tag="PLB25_14815"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HOE42880.1"
                     /translation="MAKRDYYEVLGVPKNASEDEIKKAYRKHAMKHHPDRNQGDAAKA
                     AEEKFKESKEAYEMLSDPQKRAAYDQHGFAGVDPNMRGGPGGAEAYGGFAEAFGDIFG
                     DMFGQQRGRAGGGRQVYRGSDLSYAMEVTLEEAAKGKDAQIRIPSWEACDTCHGSGAK
                     PGTQVKSCGTCGGSGSVQMRQGFFSVQQTCPTCRGTGKVIPEPCPACHGQGKIKKQKT
                     LEVKIPSGIDGGMRIRSAGNGEPGTNGGPPGDLYIEIRLKKHDIFERDGDDLHCSVPI
                     SIVTATLGGEIDVPTLAGKAAIDVPEGTQTGKQFRLRGKGIKGVRSSYPGDLYCHILL
                     ETPVKLTEHQRKLLRELDESLKKGGAKHSPTGDSWTDRLKSFFS"
BASE COUNT         1171 a         1272 c         1374 g          926 t
ORIGIN      
        1 gcaagacagc gtcggcggca tcacgcccaa tacgctcaat actcgcataa aagagcttcg
       61 cgaagctgat ctcattaccc atggcaacaa aggctatctg gtcaccgctg cgggcgctga
      121 cctgctcaaa cgcatgggcg atttgtctgc ctttgccacc aaatgggcca gcggccaggc
      181 taaaaaagcc gtctgatttg cctgacattc cacaccattt tttcacccaa cattgacctc
      241 catgactacc acgccctccg gacttgaata tgacgacaac acaactggca ccggcgacct
      301 tgccgccaaa gggcacgagg taaccgttca ttacaccggc tggctctatc aggacggcgt
      361 tcagggccca aagttcgatt ccagcaaaga ccgccaaagc ccgtttgtct tcaccttggg
      421 tgctggcatg gtcatcaaag gctgggacga aggtgtcgcc ggcatgtgtg ttggtggcgt
      481 acgtacgctg attattccct ccaccctggg ttacggtgcg cgtggtgctg gcggtgtcat
      541 tccaccgaat gcaaccttga agtttgaagt ggaactgctt ggcgttcgct gaagggtcag
      601 tggtgagacg tgggtgatcg aggcatcacc agagcgccag cgtagcgtca acatcagaat
      661 ggtcagctat cagcgcgtgg tttttttggg tttcggcgtt gatcgctgtt ctggggggct
      721 tgaaaattcg ttgcccgcct ccaagtccaa gcttctttgt atttaaacca tctccgaatg
      781 attgacccta caccgcatac tactggcgcc gacaattcgg cggcccatac cgaaacagca
      841 gcgcccgatc agcaggacat ccagaatatc accgctgcga tggccaacta cgaggccgag
      901 gcgctcaaca aatgccaagc cgatctggct cagttgcagg ctaaaagcgc agagttggct
      961 gatcaattct tgcgcgccaa ggcagatgct gaaaatgccc gtcgtcgagc ggaagacgat
     1021 gtttccaagg cacgcaagtt tgccattgaa ggtttcgccg acagtttgtt gcctgtgatc
     1081 gacagcctgg aagctggctt gggggtgaag gaaggtgcgc tggaggcgat ccgtgaaggc
     1141 gcagaagcta ccttgaggca actgctggcc gcgctggagc gcaacaaggt tgtggtcatc
     1201 aatccggcac ctgcggcgaa gttcgatcca cacctgcacc aggctatcag cgtggtgcca
     1261 agtgaccagg agcccaacac cgtggtgatg gtgctgcaaa aagggtacct gatctccgaa
     1321 cgtatcctgc gaccagccct ggtcacggta tcgggcccga aataggcatc cgatccggct
     1381 tgaaatactt caagtcatcc acaagttatc aacatctgaa aaaatttagg agttcctcat
     1441 gggcagaatc atcggcatcg atctgggcac caccaacagt tgcgtggcca tcatggaagg
     1501 caatagcccc aaggtgatcg aaaactcgga aggtgcacgc accacaccat ccatcattgc
     1561 ttaccaggaa gacggcgaag ttctggtcgg tgcatctgcc aagcggcaag ctgtcaccaa
     1621 ctcgaaaaac accctgtacg cagtaaagcg cctgattggt cgcaagttca ccgaaaaaga
     1681 ggtgcaaaaa gacatcaacc tgatgcccta caaaattgcc gctgcagaca atggcgatgc
     1741 gtggatcgag gttcgtggca ctcgtctggc accccagcaa atcagtgctg atgtactgcg
     1801 caagatgaaa aagactgccg aagactacct tggcgaggcc gtgaccgagg cggtgatcac
     1861 agtgccagcc tacttcaacg acgcccagcg ccaagcgacc aaggatgctg gccggattgc
     1921 cggcctcgaa gtcaagcgca tcatcaacga gccaacggcg gctgcgctag cgtttggttt
     1981 ggacaagaac gaaaagggcg accgcaaaat cgcggtgtat gacctaggcg gtggaacatt
     2041 cgatatttcc atcatcgaga ttgccgatgt ggacggcgaa aagcagtttg aagtgctgtc
     2101 caccaatggc gacaccttcc tgggcggcga agactttgac cagcgcatca tcgacttcat
     2161 catcgccgag ttcaagaaag agcagggcgt tgatctgagc aaggatgtac tggccttgca
     2221 acgcctgaag gaagcggctg agaaggccaa gatcgaactc tccagcagtg cccagaccga
     2281 catcaacctg ccctatgtga cggctgatgc atctggaccc aagcacctga gcatcaagtt
     2341 gacacgctcc aaactggagt cgctggtcga agagttgatc gagcgcacca tggctccctg
     2401 ccgcactgcc atcaaggatg cgggtgtcag cgcggccgac attcatgacg tgattctggt
     2461 cggtggcatg acccgcatgc ccaaggttca ggagaaggtc aaggaactgt ttggcaaaga
     2521 accccgcaag gacgtgaatc ccgacgaggc ggttgcggtg ggtgccgcca ttcagggcca
     2581 ggtgctctct ggtgatcgca aggacgtgct gctgctcgac gtgacaccgc tgtcgctcgg
     2641 cattgagacc ctgggtggtg tcatgaccaa aatgatcacc aagaacacca cgataccgac
     2701 caagtttgcc caaacctttt cgactgccga cgacaaccag ccagcggtga ccatcaaggt
     2761 gtttcagggt gaacgcgaga tcgccgctgg caataaactg ctgggcgaat tcaaccttga
     2821 aggtatccct ccggcagcgc gcggtttgcc gcagattgag gtgtcgtttg acattgatgc
     2881 caatggcatt ttgcatgtcg gtgccaaaga caagggtacc ggcaaagaaa acaagatcac
     2941 catcaaggcg aattcgggtc tgtcagaggc cgagattcag caaatggtca aggatgccga
     3001 actcaatgct gcggacgaca agaaaaaact tgagctggtt caggcccgca atcaagccga
     3061 ggccagtctg cacagcgtca agaagagcct gaccgagtat ggtgacaagc tcgatgctgg
     3121 cgaaaaggaa aaaatcgagg ccgctgtcaa ggatctggaa gcggttttga aaggcgaaga
     3181 caaggccgcc attgacgaca agagtgcagc cctgatgacc gtaagccaga agctcggcga
     3241 gaaaatgtat gctgatatgc aggccaagca ggctgctgaa gggaactctg gcgctgatgg
     3301 tgcgcaggca tcgccgcaaa gtggcaccgc acaagacgac aatgtggtgg atgccgaagt
     3361 gaaagaagtc aaaaagccct gagcgctggc ccgatcactg ttgtagacat atgcaacaaa
     3421 cgccgcgttg attgcctttt ggcttcaacg cggcgtttgt attgaaacca ggtacgagta
     3481 gaccatggca aaaagagatt attacgaagt tctgggcgtt ccaaaaaacg cttccgagga
     3541 cgagatcaag aaggcttatc gcaaacacgc gatgaagcac catcctgacc gcaaccaggg
     3601 cgatgctgcc aaagcggctg aagaaaagtt caaggaaagc aaggaagcct acgaaatgct
     3661 gtcggacccg caaaagcgtg cggcttatga ccagcatggt tttgccggtg tggaccccaa
     3721 catgcgcggc ggtccgggcg gcgctgaggc ctatggtggc tttgccgaag cttttggcga
     3781 tatttttggt gacatgtttg gccagcagcg tggtcgcgcc ggcggtggcc gtcaggtcta
     3841 tcgcggtagc gatttaagct acgccatgga agtcaccctg gaagaagccg ccaagggcaa
     3901 ggacgcgcaa atccgtatcc cgagttggga agcctgtgat acctgtcatg gcagcggagc
     3961 caagcctggc acccaggtca agtcttgcgg cacctgcggt ggcagtggtt cggtgcaaat
     4021 gcgccagggc tttttcagcg tgcagcaaac ctgcccgacc tgccgtggca ctggcaaggt
     4081 cattcctgaa ccctgcccag cctgccatgg ccagggcaag atcaagaaac aaaagacgct
     4141 ggaggtcaaa attccttcgg gtatcgacgg tggcatgcgt attcgcagcg ccggcaatgg
     4201 cgaaccaggc accaacggtg ggccgccggg tgacctgtac attgaaattc gcctcaagaa
     4261 acacgatatt ttcgagcgtg acggcgacga tcttcattgt tcggtaccca tcagcatcgt
     4321 caccgccacc ctgggtggcg aaatagatgt gcccacactg gctggcaagg cggccattga
     4381 tgtgcctgaa ggcacccaga ccggcaaaca attcagactg cgtggcaagg gcatcaaggg
     4441 tgttcgttcc agttacccag gcgatttgta ctgtcacatc ctgcttgaga caccggtcaa
     4501 gctgaccgag catcagcgca agctgctgcg tgagctcgat gaatcgctca agaaaggtgg
     4561 ggccaaacat tcccccaccg gggacagttg gacagatcgt ttgaagagct ttttcagcta
     4621 ggagcctgtc tggtctttgg tgtcgagtgc gaaaatcgac accagcgagg caggttttac
     4681 ctgatttgct ggccgaaagt tgatctattg gtcagagggt cggcgcaaaa agggtcgttg
     4741 cgg
//
LOCUS       DAOSLL010000369         4740 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16014_length_4740_cov_3.795518, whole genome shotgun sequence.
ACCESSION   DAOSLL010000369 DAOSLL010000000
VERSION     DAOSLL010000369.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4740)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4740
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16014_length_4740_cov_3.795518"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="PLB25_14820"
     CDS             <1..308
                     /locus_tag="PLB25_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HOE42881.1"
                     /translation="ANQLMDYLENGNITNSVNFPAVSMARSAGLTRITFSNNNVSGVL
                     GQVLSVLAGNQVNVIDMMNKSRGELAFNLIDVESRPADDVLLAIAAVQHVIRVRVIG"
     gene            complement(821..2284)
                     /locus_tag="PLB25_14825"
     CDS             complement(821..2284)
                     /locus_tag="PLB25_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE42882.1"
                     /translation="MSPSTCPADFEHLFESAPISLWLEDYSALKTLFEQWREQGVTDF
                     GAFLRASPGRLAQCSGCLKVIKVNQKTLQVFAAADQQQLVSRLCEVFRDDMFDNMVTE
                     LEHLWRGELEFANQTVNYGLDGRRIDVQIQVRVLQGHETDWTRVMVSLQDITPQVQAR
                     LELQRSERYARDLFEHSPVSLWVEDFSGVKVLLDEARAKGIQDFRVFLSVHPDFVTRC
                     LEKIRVIDINQQTLKMFAARSKDELLGNLGSVFRGEMQNSFAEQLQDLWNGKLTQQRE
                     VINYSLTGDVINIHLQFSVLQLHEARWDQVLISLVDITARKKAEAYLEYLGKHDSLTG
                     LGNRAFYVEELNRLSRKGPWPLSLLAIDLNSLKGVNDNQGHAAGDALLRRAGEVLASA
                     TNGQTACTARIGGDEFVVLLPGTDERGAQSLKARIQSMTDLNNQFYPGQRLSMAIGIG
                     LCSGPGLVDAALNQADQDMFAAKTQYYTDMQIERRRY"
     gene            complement(3143..4465)
                     /locus_tag="PLB25_14830"
     CDS             complement(3143..4465)
                     /locus_tag="PLB25_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HOE42883.1"
                     /translation="MNHFCPLPLTLALGTLLALPVQAQSLVDLYESARGFDAGFQSAK
                     LQYDANLARAAQARAAILPTVGLAMGVTRSNISSDTAVFDRGSFGNQSATVNASQPLY
                     RPANVALVEQGEKQIELAKAQLTVADQDLVIRVSQAYFDVLASDDSLNLVRAQKTAVA
                     EQLASAKRNFEVGTATIVDTREAQSKFDLVLAQEIAAENDLRVKKVTLDQLSGKTDAA
                     PKPLSVPVAMVPLAPDDINQWVAQAEEFHPGILQAKANVDIAELETEKAKAGNKPTLD
                     LTGSYGVTRNNGTTNSTLDYRTNVATIGLALNLSLFAGYATQNRIKETLALEEKSRSD
                     LESAKRGVALATRTAFLGLQSGLGQVKALEAAEVSSQSVLDSTKLGYEVGVRINIDVL
                     NAQTTLFDTKAKLAKARYDVLLGGLKLRQANGTLKAEDINSVNSLLVR"
     gene            complement(4533..>4740)
                     /locus_tag="PLB25_14835"
     CDS             complement(4533..>4740)
                     /locus_tag="PLB25_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42884.1"
                     /translation="PSDDPLPLMLAEPRQLGDVFQLIGVHLSQARRASPVRNFRARFP
                     WLLANIAGGIAAWATELDPAVPTY"
BASE COUNT         1024 a         1366 c         1362 g          988 t
ORIGIN      
        1 ccgccaacca gctcatggac tacctggaaa acggcaacat caccaactcg gtgaatttcc
       61 cggcagttag catggctcga agtgccggct tgactcgcat cacgttttcc aataacaacg
      121 tctcgggcgt gcttgggcag gtgctatcgg tgctggctgg caaccaggtc aatgtcatcg
      181 acatgatgaa caagagccgg ggcgaactgg ctttcaacct cattgatgtg gaaagtaggc
      241 ccgccgacga tgtgctgctg gccatcgcag cagtgcaaca cgtgatccga gtgcgggtta
      301 ttggctagcc ttgatggtgc ccaggccagg cattgcgcaa ttggcctacc tggcccacca
      361 gtgcatgcat cgacaaccgg cctagtcact gcggcaacgc cccttcgtcc ggggcaccat
      421 gtttggcgcg gccgggcctg catccgagat tcctcagttg gaagcgcccg cgcgggccgc
      481 tggcgagctg ctcggggtgg cgacgacgcg taacgcctga atacaagtcg tcctggcaga
      541 cgagtcaggc gcaactcccc caggcgcgtt gcaagcggtt caatcggatg agcagcgctg
      601 tgactcaatg ggtgaacccg atcaaagctg aaaaggtcag tattcatgag ggcttacaaa
      661 ggaaaacaag cagtcaactg aggaatctcg gacgatctga aacaggccat cagcgcggaa
      721 cctggtcgtc ggcaagggtg accagcagcc agcatcgttg agtcgccaaa tcaccccctg
      781 ttttttcaag agcgccccgc ccgccgacgc gctggtacgc tcaatagcgg cgacgctcga
      841 tctgcatgtc ggtgtaatac tgagttttcg cggcaaacat gtcctggtct gcctggttca
      901 gcgctgcatc aaccaaaccc gggccgctgc acagcccaat gccaattgcc atgctcaggc
      961 gctggcccgg ataaaactgg ttgttcaggt cggtcatgct ctgaatgcgc gctttaagcg
     1021 actgcgctcc gcgttcatcg gtgccaggca gcagcacgac aaattcgtcg ccaccaatgc
     1081 gcgcggtaca agctgtctgg ccgttggtgg cgctggccag cacctcgcca gcccgtcgga
     1141 gcaaggcatc gccggccgcg tgtccctggt tgtcgttgac acccttgagg ctgttgagat
     1201 caatggccaa caaactcaat ggccacggac ccttgcgcga taggcggttg agttcctcga
     1261 catagaaggc ccgattgccc aacccggtaa gcgaatcgtg cttgccgagg tattccagat
     1321 aggcctcggc ttttttgcgt gcagtgatgt ccaccagcga aatcaacacc tgatcccaac
     1381 gcgcctcgtg caactgcaac accgagaact ggaggtggat gttgatcaca tcgccggtca
     1441 acgaataatt gatcacctcg cgctgttgcg tcagtttgcc gttccacagg tcttgcaact
     1501 gctcggcaaa tgagttttgc atttcgcccc gaaagaccga gccgaggttg cccagcagtt
     1561 cgtccttgct gcgcgcagca aacattttga gggtctgctg gttgatatcg ataacccgga
     1621 tcttttccag acagcgcgtg acaaagtccg ggtgtacgct gagaaacacc cgaaaatcct
     1681 gaatcccttt ggcgcgcgcc tcatcaagca acaccttgac accactgaag tcctcgaccc
     1741 acagcgacac cggcgaatgt tcaaacaggt cacgcgcata gcgctcgctg cgttgcagtt
     1801 ccagccgggc ctgcacttgc ggcgtgatgt cctgcagcga caccatcacc ctggtccagt
     1861 cagtctcgtg cccctgcagc acgcggacct gaatctgtac gtcgatgcgc ctgccgtcaa
     1921 gcccatagtt gacggtctga ttggcaaact ccagttcgcc gcgccacaga tgttccagtt
     1981 cggtcaccat gttgtcgaac atgtcgtcac gaaacacctc gcacaggcgt gaaaccagtt
     2041 gctgctgatc ggcagcggca aacacctgca gcgttttctg gttgaccttg atgactttga
     2101 gacagcctga acattgcgca agccgaccgg gtgaggccct cagaaacgcg ccaaagtcgg
     2161 tgaccccctg ctcacgccac tgctcaaaca gggtcttgag tgcgctgtag tcttccagcc
     2221 aaagtgaaat tggggcagat tcgaacaggt gctcgaagtc cgcaggacag gtggatggtg
     2281 acatgctgaa agtcccggcg aataaagttt tgaaaatagc gctggttgga aagaatactt
     2341 ggcttgtcgc ggtcgaaatg gtggtttgaa gacaaagcct ggctgatcgc tatcggattg
     2401 tgcgccagcc cacgcggtgc atcactcacc tgctgatatc tagacctcca ggctcatcaa
     2461 ccatcaagca aatttatccc agccgatgcc tagacttgtt ggacatagaa aatgacaggt
     2521 gcagtgattt tggggacgga tcgcaattgc ggcagcacat gggcaacgca aaaaaaaaca
     2581 gcgtcgaaaa gcaattctct cagacaccaa tatcactcgt caatgcccca gaatttatca
     2641 aaaacaatag ctgccagagc ttattccaca aaggctacag ggcaatttct catgaaaata
     2701 ataaagagtc ctgcaatccg aacggcagca tcgaatcagc ctcaccgagc tgattgggcc
     2761 aaccagtagt ttgtccgttg aggtaccggt attggttgca gcaccactcc gatccacatc
     2821 gaaatggcat ggctgccttg gcccgtgaaa ctggagccca attttctgca gtgccgggat
     2881 tcttgccgca ggctagtggc cagagattgg tatccgatgc aaaaatttgc tgcacctgca
     2941 gcgcaaattg acaagctctt gcctagctga tcttcaggaa gagtacaaaa aacagtcaca
     3001 gtcaccagag caggatcaat gcatcaacct tgttttcccc cccatgtgca gcgatggttt
     3061 tctgcccgag cgcaagcacc ggggcagatg gggaacggcc agaaccactg acccggcagg
     3121 atcgatacgg cctgccgctt ggttacctga ccagcagcga gttaaccgag ttgatgtctt
     3181 cagcctttag cgtgccattg gcctggcgca acttcagccc accgagcaac acgtcatagc
     3241 gcgccttggc caatttggcc ttggtgtcaa acagggtggt ttgggcgttg aggacgtcga
     3301 tgttgatgcg caccccgact tcgtagccca gtttggtcga gtcaagcaca ctttggctgg
     3361 aaacttcagc ggcctccagc gccttgacct ggcccaggcc ggattgcagg cctaaaaaag
     3421 ccgtgcgcgt ggccaaggcc acgccgcgct tggctgattc aaggtcggat cgggattttt
     3481 cttcaagcgc cagggtttcc ttgatgcgat tttgcgtggc gtagccggca aacagcgaca
     3541 ggttcaatgc caggccaatg gtcgcgacgt tggtgcggta gtcgagcgtt gaattggttg
     3601 tgccattgtt tcgcgtcaca ccgtagctgc cggtcaggtc aagtgttggc ttgttgcccg
     3661 ccttggcctt ttcggtttcc agttcggcga tgtcgacatt tgccttggct tgcagaattc
     3721 cgggatgaaa ctcctcagcc tgtgcgaccc attggttgat gtcgtctggt gccaacggca
     3781 ccattgcgac cggcacactc agaggcttcg gcgctgcgtc ggttttgccg gagagttgat
     3841 caagggtcac tttcttgacg cgcaaatcgt tttcagcggc gatttcctgc gccagcacca
     3901 ggtcaaattt tgactgcgcc tcgcgcgtgt caacgatggt ggcggtacct acctcaaagt
     3961 tgcgcttggc tgaggccagt tgttctgcca cggcggtctt ttgcgctcgg accaagttca
     4021 ggctatcgtc cgaagccagc acgtcaaagt aggcctggct gacgcgaatg accagatcct
     4081 ggtcggccac agtgagctgg gctttggcca gttcgatctg cttttcacct tgctcgacca
     4141 gcgccacatt ggccggtcgg taaagcggct gcgaggcatt gacggtggcg ctctggttgc
     4201 caaaactgcc ccgatcaaaa acggcggtgt cactgctgat gttggagcgg gtgactccca
     4261 tggccaggcc gacagtgggc aaaatcgctg cacgggcctg tgccgctctg gccaggttgg
     4321 catcgtattg cagcttggct gactggaaac cggcgtcaaa tccccgggcc gactcataca
     4381 ggtcaaccag gctttgcgcc tggacaggca aagccagcag ggtgcccaac gccagggtca
     4441 ggggaagcgg acaaaagtgg ttcatggtgt gcctgaaaaa atggggacga ctcggggact
     4501 gacggagcgc aggggagtat ttgccgctga catcagtagg tcggcactgc ggggtcgagt
     4561 tcagtcgccc aggcagcaat gccgccggcg atgttggcca gcagccacgg gaatctcgcc
     4621 cgaaagttcc gcaccggcga cgcccggcgg gcctgcgaca gatgcacgcc gatgagctga
     4681 aacacgtcgc cgagctgccg cggctcggcc agcatcagcg gcagcggatc gtccgaaggc
//
LOCUS       DAOSLL010000370         4738 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16019_length_4738_cov_4.713004, whole genome shotgun sequence.
ACCESSION   DAOSLL010000370 DAOSLL010000000
VERSION     DAOSLL010000370.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16019_length_4738_cov_4.713004"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PLB25_14840"
     CDS             <1..425
                     /locus_tag="PLB25_14840"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOE42885.1"
                     /translation="DLAGAYAFGHYTHEKNPVTAAAALATLDIIEREQLVDNAASVGA
                     YALERLQDMRRQHPLIGDVRGRGLLIGIELVTDRAAKTPATDVAEAVLYAAMSRGLSF
                     KTTMGNVLTWTPPLITSHEQMAQALDILDQCLREIAAP"
     gene            454..1689
                     /gene="phnA"
                     /locus_tag="PLB25_14845"
     CDS             454..1689
                     /gene="phnA"
                     /locus_tag="PLB25_14845"
                     /EC_number="3.11.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047400 - phosphonoacetate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetate hydrolase"
                     /protein_id="HOE42886.1"
                     /translation="MNTAKSPSLNVNGRSYQLPKHPTVVVCVDGCEPDYLAQAVASGQ
                     MPWLKRTLAHGTALVADCVIPSFTNPNNLSIVTGVPPSVHGICGNYLFDTASGTEVMM
                     NDVKWLRAPTLLAALSDAGLKLAVVTAKDKLRELLGHKMTGICFSAEKADQATLAVNG
                     IDHVLALVGKPLPSVYSADLSEFVFAAGVKLMEIGRPDVMYLSTTDYIQHKHAPGTAG
                     ANAFYAMMDGYLGQLDAMGCVIALTADHGMNAKTAMDGTPDVIYLQDWFDAWLGAAQA
                     RVILPITDPYVVHHGALGSYATVYLPDGVDVTEACERLRQVLGLELVLTRTQAAARFE
                     LPADRIGDIVVVSERATVIGTSVARHDLSALEVPLRSHGGISEQRVPLIVNRRLPGLD
                     VTKRWRNFDAFDLVLNHVA"
     gene            2151..3569
                     /gene="phnY"
                     /locus_tag="PLB25_14850"
     CDS             2151..3569
                     /gene="phnY"
                     /locus_tag="PLB25_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017232627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde dehydrogenase"
                     /protein_id="HOE42887.1"
                     /translation="MRLAGRKVGADRGGERCIKVFNPFTEEVIGTVPKATLDEVRETF
                     AAARSYQPKLTRFERAAILNRAAAIISQRLDEVAQLITAEAGLCIKDALYEAGRVSDV
                     LLFGASECLKDDGQIFSCDLTPHGKKRRVYTQRGPLLGVITAITPFNHPMNQVAHKVV
                     PSIASNNRMVLKPSEKVPFSAILFADILYEAGLPPECLSVITGDPREIADELISNEHV
                     DLVTFTGGVAIGKYIAAKAGYRRMVLELGGNDPIIVMEDADIDEASTLAVQGSYKNSG
                     QRCTAVKRMLVHSAVADEFVEQVVTKTIAWQYGNPMDQRNDMGTVIDEPAAMFFESRV
                     NEAIGQGASLRVGNLRRGALYSPTVLDHVLPDMRVAKEETFGPVSPIIRFDSIDQAIA
                     ISNGTAYGLSSSVCSNRLDYITRFINELQVGSVNVREVPGYRLELTPFGGIKDSGLGY
                     KEGVQEAIKSFTNLKTYSLPWV"
     gene            3902..>4738
                     /locus_tag="PLB25_14855"
     CDS             3902..>4738
                     /locus_tag="PLB25_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006756956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE42888.1"
                     /translation="MRPFWIEQALFNAGDLAPALQGDIKADICIVGGGYTGLWTAIQT
                     KQQHPALDIVILEGDLCGAGASGRNGGCVLTWSAKFFTLHRLFGESEAIRLVRASETA
                     VQQIADFCRAHRIDAEFRHDGTLYTATNPAQVGTLEPVMQALESCGIESYYRLSPAEV
                     AHRSGSASNLAGVYSPHAATVHPGKLVRGLRRVALAMGIRIFERSPMLGFDTGQPVSV
                     RTPGGSVTADKLVLATNAWMAGMFAQFERSIVIVSSDMVITDKCPALLAQTGLVDGVS
                     VLD"
BASE COUNT          934 a         1436 c         1433 g          935 t
ORIGIN      
        1 tggacctggc aggcgcatat gcctttggcc attacacgca tgaaaaaaac ccggtcaccg
       61 ccgccgccgc gctggccacg ctggacatca ttgagcgcga gcaactggtt gacaacgccg
      121 cgagcgtcgg cgcttatgcg ctggagcgcc tgcaagacat gcgcaggcag catccgctga
      181 ttggcgacgt gcgcggccgt ggtctgctga ttggcattga actggtgacc gaccgcgccg
      241 ccaaaacacc tgccactgat gtggctgaag cggtgctgta cgccgccatg tcgcgtggcc
      301 tgtcgttcaa gaccaccatg ggcaacgtgc tgacctggac cccgccgctg atcaccagcc
      361 acgaacaaat ggcgcaggcg ctggacattc tggaccaatg cctgcgcgaa attgccgcac
      421 cctgatcaaa cctcaccctg aaagccacca cccatgaaca ccgccaaatc ccccagcctg
      481 aacgtcaatg gccgcagcta ccaactgcca aagcacccca ccgtggtggt ctgtgtcgat
      541 ggctgcgagc ctgactacct ggcacaggcc gttgccagcg gccagatgcc gtggctcaag
      601 cgcacgctgg cgcacggcac cgcgctggtg gccgactgcg tgattccgag ctttaccaac
      661 cccaacaacc tgtccatcgt gaccggtgtg ccgcccagcg tgcatggcat ttgtggcaat
      721 tacctgttcg ataccgccag cggcactgaa gtcatgatga acgatgtcaa gtggctgcgt
      781 gcgccaaccc tgctggctgc actcagcgat gccgggctga aactggcggt ggtgaccgcc
      841 aaggacaagc tgcgcgaact gctcggccac aaaatgaccg gcatctgctt ttcggcagaa
      901 aaagccgatc aggccacgct ggcggtcaac ggcatcgacc acgtgctggc gttggttggc
      961 aaaccgttgc ccagcgttta cagcgccgac ttgtccgaat ttgtctttgc cgcaggtgtc
     1021 aagctgatgg aaattggtcg gcccgatgtg atgtacctca gcaccaccga ttacatccag
     1081 cacaagcacg cgcccggcac ggccggggcc aatgcgtttt acgccatgat ggacggctat
     1141 ctgggccagc tcgatgccat gggctgcgtg attgccctga ctgccgacca cggcatgaac
     1201 gccaagaccg ccatggacgg cacaccggat gtgatctacc tgcaagactg gtttgatgcc
     1261 tggctgggtg cggcgcaggc acgggtgatt ttgccaatca ccgatcccta tgtcgttcac
     1321 catggcgcgc tgggctctta tgccacggtt tatttacccg acggtgtcga tgtgaccgaa
     1381 gcctgcgagc gtttgcgcca ggtgttgggg ctggaactgg tgctgacgcg cacgcaggcg
     1441 gccgcccggt ttgagctgcc ggccgaccgc attggcgaca tcgtggtggt gtcggagcgc
     1501 gctaccgtga ttggcacctc ggtggcacgg cacgacctga gtgcgctgga ggtgccgctg
     1561 cgctcgcacg gcggcatcag cgagcagcgc gtgccgctga tcgtcaaccg ccgtctgcct
     1621 gggctggatg tgacgaagcg ctggcgcaat tttgatgcgt ttgatctggt gctgaatcat
     1681 gttgcgtgat cattcatgct ctatttagca tagctgcttg cgcttgtttc ataagggctg
     1741 gaaggtgatt tgccatgtat aacaggctga atttcggctt gcctcatccg gctttggcgg
     1801 gcagcttggg gttggcccgc agaattgggg tcagagccca attcggtgat gccttgttgg
     1861 gtttgcgggc agcgttgggc cgaattggga tccgacccca aatctgctac ggcgtggcag
     1921 aaattgaccg aggcgatggt cggctgtcgc tagggggccg gtttcagtct ggacggcggg
     1981 cgctggcctg gacctgcttc gactgtcatc agggcggtgc caacggttcc ttgaatacta
     2041 ttattaaaat agctatcagc gcttattcca taagggttgt aggccaattt cttgtataaa
     2101 acaccatgaa tgacatgcag caatacatcc gtgaccatca actcaacgcc atgcgcctgg
     2161 ccggtcgaaa ggtcggggca gatcgtgggg gcgaacgctg tatcaaggtg ttcaacccgt
     2221 tcactgaaga agtcattggc acggtgccca aggccacctt ggacgaggtg cgcgaaacct
     2281 ttgctgcggc gcgcagctac cagcccaagc tgacccggtt tgagcgcgcc gccatcctga
     2341 accgggctgc cgccatcatc agccagcggc ttgacgaggt ggcgcaactc atcacggcgg
     2401 aggcaggttt gtgcatcaag gatgcgctgt acgaggccgg tcgggtcagc gacgtgctgc
     2461 tgtttggtgc cagcgaatgc ctgaaagacg acggccagat tttcagttgc gacctgaccc
     2521 cgcacggcaa aaagcgccgc gtttataccc agcgcggccc gctgctgggt gtcattaccg
     2581 ccatcacccc gttcaaccac ccgatgaacc aggtggcgca caaggtggtg cccagcattg
     2641 ccagcaacaa ccgcatggtg ctcaagccgt ctgaaaaagt gccgttttcg gccatcctgt
     2701 ttgccgacat cctgtatgaa gctggtttgc cgcccgaatg cttaagtgtc atcaccggtg
     2761 acccgcgcga gatcgccgac gagctgatca gcaacgaaca cgtcgatctg gtcaccttca
     2821 ccggcggtgt ggcgattggc aagtacattg ctgccaaggc cggttaccgg cgcatggtgc
     2881 tggagcttgg cggcaatgac ccgatcatcg tgatggaaga tgccgatatc gacgaagcct
     2941 cgacgctggc ggtgcagggc agctacaaga attcgggcca acgctgcact gcggtcaaac
     3001 gcatgctggt gcattcggca gtggccgatg agtttgtcga acaggtggtg accaagacca
     3061 ttgcctggca atatggcaac ccgatggatc agcgcaacga catgggcacg gtgatcgacg
     3121 agccagccgc gatgtttttt gaaagccggg tcaacgaggc catagggcag ggcgccagcc
     3181 tgcgggttgg caatctacgc cgtggtgcgc tgtattcacc gaccgtgctc gaccatgtgc
     3241 tgcccgacat gcgggtggcc aaggaagaaa cctttggccc ggtgtcgccg atcatccgct
     3301 ttgacagcat cgaccaggcg attgccatca gcaacggtac ggcatacggc ttgtcaagct
     3361 cggtgtgcag caaccggctc gactacatca cccgcttcat caacgaactg caggttggca
     3421 gcgtcaatgt gcgcgaagtg cccggctacc ggctcgaact cacccccttt ggcggcatca
     3481 aagactctgg cctgggctac aaggaaggtg tgcaggaagc catcaagagc tttaccaacc
     3541 tcaaaaccta ctccttgccc tgggtttgat gccagatcag catggacaac gcccgccaat
     3601 gtcaacaaaa cacctggcaa gttcctcaga gtgaagctgc agcaagtgtt gcaaatattg
     3661 tcctgtcttt atcctttcat caacagggcg caagtgctag cagatttggt agcagatttg
     3721 gggtcggatc ccgattcggc caagtgctgc caaaccagcc agcaatgcat caccgaatcg
     3781 ggctcagacc ccaattctgc gggccttacc cacgcggcat cggcgagttt tcaaaatttc
     3841 ggttgaagca atggttccaa tgaaaactga aaaacacgca accccctaca cgccaaccgc
     3901 catgcgcccg ttctggatcg agcaggcctt gttcaatgcc ggcgatctgg ccccggcctt
     3961 gcagggcgac ataaaggccg acatttgcat cgtcggcggc ggctacaccg gcctgtggac
     4021 tgccatccag accaagcagc agcacccggc cctggacatc gtcattctgg aaggtgacct
     4081 gtgcggggcc ggtgccagcg gacgcaacgg cggttgtgtg ctgacctggt cggccaagtt
     4141 tttcacgctg caccggctgt ttggcgagtc cgaagcgatc cggctggtca gggcctctga
     4201 aacagcggtg cagcagattg ccgacttttg ccgggcgcat cgcatcgacg ctgaatttcg
     4261 ccacgacggc accctttaca ccgccaccaa cccggcgcag gtcggcacgc ttgagccggt
     4321 catgcaggcg ctggagtcgt gcggcatcga gtcgtattac cggctgtcgc ctgcagaagt
     4381 ggcgcaccgt tccggctcgg ccagcaacct tgccggggtt tattcaccgc atgctgccac
     4441 cgttcacccc ggcaagctgg tgcgtggcct gcgtcgcgtg gcgcttgcca tgggcatccg
     4501 cattttcgag cgcagcccga tgctcggctt tgacaccggc cagccggtca gcgtgcgcac
     4561 ccccggcggc agcgtcaccg ccgacaagct ggtgctggcc accaacgcct ggatggcggg
     4621 catgtttgcg cagtttgagc gcagcatcgt catcgtctcc agcgacatgg tcatcaccga
     4681 caaatgcccg gcgctgctgg cgcaaaccgg cctggttgac ggcgtgtcgg tgctcgat
//
LOCUS       DAOSLL010000371         4716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16113_length_4716_cov_5.323536, whole genome shotgun sequence.
ACCESSION   DAOSLL010000371 DAOSLL010000000
VERSION     DAOSLL010000371.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4716
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16113_length_4716_cov_5.323536"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(129..710)
                     /gene="metW"
                     /locus_tag="PLB25_14860"
     CDS             complement(129..710)
                     /gene="metW"
                     /locus_tag="PLB25_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HOE42889.1"
                     /translation="MSDPQVQQALAELVPPGSRVLDLGCGDGAMLAHLQQTRGCTGYG
                     IEIDDANVQACVKRGVNVIQLNLDEGLSMFEDASFDVVLQIDTLQHLRNAEVMLLETV
                     RVGRIGVVAFPNFAHWPNRLSVLTGRMPVTRRLPFQWFDTPNIRVGTHADLGLLAQGN
                     DLKVLDSFGIQDGEVVRWFPNLLAGTSVYKLSR"
     gene            complement(707..1870)
                     /locus_tag="PLB25_14865"
     CDS             complement(707..1870)
                     /locus_tag="PLB25_14865"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HOE42890.1"
                     /translation="MPLATLNTDMIATPQSMPFTAALPLRSGASLRDYSLSFETYGTL
                     NASRTNAVLVCHALNASHHVAGVYAGQDKSEGWWDNMIGPGKPLDTDKFFVIGINNLG
                     SCFGSTGPMHNNPDTGRVYGSDFPVVTVEDWVDAQARLLDALGIQTLAAVMGGSLGGM
                     QALSWTLQYPSRVRHALVIASAPSLTAENIAFNEVARRAIQTDPDFHGGHFYQHGTVP
                     KRGLRIARMIGHITYLSDDVMNEKFGRRLLAASQSDTERDYFYSTLEAEFQIERYLRY
                     QGDKFAEYFDANTYLLITRALDYFDPARAYGGNLSLALGRASAKFLLVSFTTDWRFSP
                     RRSREIVKALLDNQRDVSYAEISARHGHDAFLLDDPRYMGVVRSYFDSMEVPS"
     gene            complement(2029..2961)
                     /locus_tag="PLB25_14870"
     CDS             complement(2029..2961)
                     /locus_tag="PLB25_14870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HOE42891.1"
                     /translation="MSQSKFRLVTRSDFDGLVCAVLLKELDLIDDIKFVHPKDMQDGK
                     IDITSRDITTNLPYVAAAHLSFDHHESETLRNTGERSNHIISAHSPSAARVVYDYYGG
                     AKAFPNISTEMMEAVDKADSAQFSHDEILQPTDWVLLNYLMDSRTGLGRFREFRVSNY
                     QLMMDLIDYCHNHNIAEILRLPDVVERVELYFDHAVRAREQIERCATVHHNLVVLDLR
                     PEETIWAANRFMIYALYPQCNISIHVLWGVQKQNTVFATGKSILDRSSKTNVGELMLQ
                     YGGGGHNAAGTCQVENDRAAAVLGELIKQINADG"
     gene            complement(3095..3658)
                     /locus_tag="PLB25_14875"
     CDS             complement(3095..3658)
                     /locus_tag="PLB25_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HOE42892.1"
                     /translation="MEAFLISTGIVALAEMGDKTQLLALILAARFRQPWPIVGGIFVA
                     TIANHALAGAVGAWTTTVLGPDVLRWVLGVSFIAMAIWMLIPDKLDDNDSPGKPRFGV
                     FGTTVVAFFLAEMGDKTQVATVMLAAKYHAYYWVIAGTTVGMMLANAPVVWLGERMTR
                     LIPLRVVHIVSALIFIGLGLWALLGRF"
     regulatory      complement(3661..3860)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00080"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="yybP-ykoY riboswitch is a manganese riboswitch that
                     directly binds Mn; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="manganese"
                     /db_xref="RFAM:RF00080"
     gene            complement(4045..>4716)
                     /locus_tag="PLB25_14880"
     CDS             complement(4045..>4716)
                     /locus_tag="PLB25_14880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE42893.1"
                     /translation="IAVIDGIAFQTNILALNAAIEAARAGEQGRGFAVVASEVRALAG
                     RSAEAAKEIKTLISASVERVEQGTTLVDQAGSTMTEVVTSIRRVTDIMGQISKASEEQ
                     SLGVSQVGQAVSQMDQSTQQNAALVEQMAAAAGSLNSQAQELVQTVAVFKLGAATSSN
                     LSRLPSTAVRAHPPKASTFKGTDRRASGTPKGAAARGTSPAQPSAKAIAVANNSTSDA
                     DWETF"
BASE COUNT          957 a         1407 c         1426 g          926 t
ORIGIN      
        1 ggcaatggca ccggcgtgaa ccggatcaaa caaggtgctg gcagagtgtg tcatgtagag
       61 tcgtcaaagt tgaacgtgca ggcacagttg ggggcgaaaa cgccaaacaa caggggccaa
      121 ccggcttgct agcgagatag cttgtaaacc gaggtacccg ccagcaaatt ggggaaccag
      181 cgcaccactt cgccgtcctg gatgccaaag ctgtcgagca ccttcaggtc attgccttgc
      241 gccagcagcc cgaggtcagc atgggtaccc acccggatat tgggcgtgtc aaaccactgg
      301 aagggcaggc ggcgtgtcac cggcatgcgt ccggtcaaca cgctcaggcg gttcggccag
      361 tgggcaaaat tgggaaaagc aaccacgcca atgcggccca cgcgcacggt ttcgagcagc
      421 atcacctcgg cgttgcgcag gtgctgcagg gtgtcaatct gcaacaccac gtcaaaggag
      481 gcatcctcga acatcgacag gccttcgtcg aggttgagct ggatgacgtt gacgccgcgc
      541 ttgacgcagg cctgcacatt ggcatcgtca atctcgatgc cgtaaccggt gcaaccccgg
      601 gtttgctgca ggtgtgccag catggccccg tcgccgcaac ccagatcgag cacgcgcgag
      661 cccggtggca ccaactcggc cagcgcctgt tgaacctgag ggtcgctcat gagggcacct
      721 ccatactatc aaagtaagag cggacaacgc ccatgtagcg ggggtcatcg agcaaaaagg
      781 catcatgtcc atggcgcgcg ctgatctctg catagctgac atcgcgctgg ttgtcgagca
      841 gcgccttgac gatctcgcgg ctgcgcctgg gcgaaaaacg ccagtcggtg gtaaagctca
      901 ccagcaaaaa tttggcgctg gcgcgtccca gtgccaggct cagattgccg ccgtaagcgc
      961 gcgccgggtc aaaatagtcc agcgcacggg tgatcagcag ataggtgttg gcatcaaagt
     1021 actcggcaaa cttgtcgccc tggtagcgca agtaacgctc gatctggaat tcagcctcca
     1081 gggtgctgta aaagtagtcg cgctcggtgt cactttggct ggccgcaagc agtcgacggc
     1141 caaatttttc gttcatcacg tcatcgctca gataggtgat gtggccgatc atgcgggcaa
     1201 tgcgcaggcc gcgcttgggt accgtgccat gctggtaaaa atgcccgcca tgaaaatcgg
     1261 ggtctgtctg gatggcgcgc cgggccacct cgttgaaggc aatgttctcg gccgtcaggc
     1321 tcggggcgct ggcaatcacc agcgcgtggc gtacccggct cgggtactgc agcgtccagc
     1381 tcagcgcctg catgccacca aggctgccgc ccatcacggc ggccagcgtc tggatgccca
     1441 gcgcatcgag cagtcgggcc tgtgcatcga cccagtcttc gactgtcacc accggaaaat
     1501 cagaaccgta aacccgacct gtgtcggggt tgttgtgcat cgggccggtg gagccaaaac
     1561 aggagcccag gttattgatg ccaatgacaa agaatttgtc cgtgtcgagt ggcttgcccg
     1621 ggccaatcat gttgtcccac cagccctcgg acttgtcctg accggcgtaa acgccagcca
     1681 catggtgcga ggcattgagc gcatgacaga ccagaaccgc gttggttcgg ctggcattga
     1741 gcgtgccata ggtctcgaaa ctcagggaat agtcacgcag actcgcaccg cttcgcagtg
     1801 gcaacgcggc tgtgaaaggc atcgactggg gtgtagcgat catgtcggta ttgagggtcg
     1861 ctaaaggcat gaccgggttg gcaggcatct ttagcggtat ttgttatgcg cccgcaattc
     1921 tggcaaatcg gcgcggtggc agtatatcaa tgccactagc cgcctgatcg gcgggccgcc
     1981 ttgtccgccc gccgcctgtg gtctagcgtc tgatgagcag ggcgggtttc agccgtcagc
     2041 gttgatttgc ttgatcagct cgccgagcac cgctgctgcc cggtcgtttt caacctggca
     2101 ggtgcccgcc gcgttgtggc cgccgccgcc gtactgcagc atcagctcgc caacgttggt
     2161 cttgctgctc cggtcgagga tcgacttgcc agtggcaaac acggtgtttt gtttttgcac
     2221 gccccacaac acgtggatcg agatgttgca ctgcgggtag agcgcgtaaa tcatgaaacg
     2281 gttggccgcc cagatggttt cttctggccg cagatcaagc accaccaggt tgtgatgcac
     2341 ggtggcgcag cgctcaattt gctcacgcgc gcgcacggcg tggtcaaagt agagttcgac
     2401 gcgttcgacc acatcgggca gccgcaagat ttcagcaatg ttgtggttgt ggcagtagtc
     2461 gatcagatcc atcatcaact gatagttgct gacgcgaaac tcgcgaaaac gccccagacc
     2521 ggtgcgcgag tccatcaggt agttcagcag cacccagtcg gttggctgca ggatttcatc
     2581 gtggctgaac tgtgccgaat cggccttgtc aactgcttcc atcatctcgg tgctgatgtt
     2641 cggaaacgct ttggcgccac cgtaatagtc ataaaccaca cgcgcggccg acggtgagtg
     2701 agccgaaatg atgtgattgc tgcgctcgcc ggtgttgcgc agggtttccg actcgtggtg
     2761 gtcgaacgac aggtgtgccg ccgctacgta gggaagattg gtggtgatgt cacggctggt
     2821 gatgtcaatc ttgccatctt gcatgtcctt cgggtggaca aacttgatgt cgtcaatcag
     2881 gtcgagctct ttgagcaaca cggcgcagac cagaccatca aagtcgctgc gggtcaccag
     2941 acggaatttg ctttggctca tagaagtcct ttgtttggcc gggtcaaata aaaattgcca
     3001 acactcattg ctgcgggcaa atgatgctaa cgcaaataag cccgccattt gtgcgccgga
     3061 cgcgaatgcg gcgcaagagt ttcccggccc gctgctaaaa ccgtcctagc agcgcccaca
     3121 accccaaacc gataaaaatc agcgccgaca cgatgtgcac cacgcgcagc ggtatcaggc
     3181 gcgtcatgcg ctcgcccagc cagaccaccg gcgcattggc caacatcatg ccgaccgtgg
     3241 tgcccgcgat cacccagtag taggcgtggt attttgccgc cagcatcact gtggcgacct
     3301 gcgtcttgtc acccatctcg gccagaaaaa acgccaccac ggtggtgcca aaaacgccaa
     3361 agcgcggctt gccggggctg tcattgtcat cgagtttgtc aggaatcagc atccagatcg
     3421 ccatcgcaat gaaggagaca ccgagcaccc agcgcagcac atccgggccc agcaccgttg
     3481 tggtccaggc accaaccgcc ccggccaacg catggttggc gatggtggcg acaaaaatgc
     3541 cgcccacaat cggccagggc tggcgaaaac gggccgcaag aatcagcgcc agcagttggg
     3601 ttttgtcgcc catttcggcc agggcaacga taccagttga gataagaaaa gcttccattg
     3661 aggttcccaa gaaaggccgg caatcaaacc cctgactgca cggcaacccc ggccaaaacg
     3721 gcgctgtgca gccaaaggtc tcgccaaaat ccacgtttgt gaacctcgct tgcgccatgt
     3781 ctgccgctgt tggcctgaca agtctgttga cgcaagcccc tctcagtgaa gaagggcggc
     3841 tactccccaa tgacagccgc attctaggtt gcaaaccagg ctgaactgat acctgagctt
     3901 tgtccaacga ggtgtgagcc tgaacggctg gcgcattgtc acgctggcgt gggcgcacag
     3961 cggggttggc accaacactg caaggcgcga cttgaaaatg cgcgactata aagatggccc
     4021 gccaatgcag gccactggca gccgctaaaa ggtttcccag tctgcgtcgc tggtcgaatt
     4081 gttggcgacg gcaatggcct tggccgatgg ctgtgccggg ctggtgccgc gtgctgctgc
     4141 acctttgggt gtgcccgagg cgcggcggtc ggtgcccttg aacgtactgg ctttgggcgg
     4201 gtgtgcccgc acggcggtgc tcggcagacg gctcaggttg gaacttgttg ccgcgccgag
     4261 cttgaacacg gcaacggtct gcaccaattc ctgcgcctga ctgttgaggc tgcctgctgc
     4321 agcggccatt tgttccacca gcgccgcgtt ttgctgggtc gactggtcca tctgtgacac
     4381 cgcctggccc acttgcgaga cacccaggct ttgctcttcg ctggccttgc tgatttgtcc
     4441 catgatgtca gtgacgcgtc ggatcgacgt gaccacctcg gtcatggtgc tgccggcctg
     4501 atcaaccagc gtggtgcctt gctcaacccg ctccacgctg gcgctgatca gcgtcttgat
     4561 ttccttggct gcctcggccg agcggccggc cagcgcccgc acctcgctgg ccaccaccgc
     4621 aaagccgcgg ccctgctcgc cggcgcgggc ggcttcaatg gcggcgttga gcgccagaat
     4681 attggtctgg aaagcaatgc cgtcaatcac cgcaat
//
LOCUS       DAOSLL010000372         4703 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16185_length_4703_cov_4.308520, whole genome shotgun sequence.
ACCESSION   DAOSLL010000372 DAOSLL010000000
VERSION     DAOSLL010000372.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4703
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16185_length_4703_cov_4.308520"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            237..476
                     /locus_tag="PLB25_14885"
     CDS             237..476
                     /locus_tag="PLB25_14885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42894.1"
                     /translation="MMLLQLRAQVTMKKKRKQQRGLRLCEYTPALSDKVAVESYEFLQ
                     VISSSLLVRHCGCIERHQAKKPSPMSKRLNSGTSL"
     gene            607..786
                     /locus_tag="PLB25_14890"
     CDS             607..786
                     /locus_tag="PLB25_14890"
                     /inference="COORDINATES: protein motif:HMM:NF014950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HOE42895.1"
                     /translation="MEDAGRGHVRKAWNHCAGSVGKAADVLRISGKTLWEKMKRLEIG
                     AYAMSGRPAIFKLLV"
     gene            835..1380
                     /locus_tag="PLB25_14895"
     CDS             835..1380
                     /locus_tag="PLB25_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HOE42896.1"
                     /translation="MTTPACLTTAWTIHAPELRKWLCRRTSNPALADDLLQDLFLKAL
                     RQGSRFCEVRNPRAWLFEVARNLLADQWRVAQHLVALPEDLAVDTDDVDTVDHLTACL
                     PRVLAELSADDREAITLCDLQGMAQADFAKAKGLSLSAAKSRLQRSRVKLRTHLSQAC
                     QVQINADGTVADFVPRPPLAD"
     gene            1503..2549
                     /locus_tag="PLB25_14900"
     CDS             1503..2549
                     /locus_tag="PLB25_14900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HOE42897.1"
                     /translation="MTTFTAPLRAWPLRQPVFFLLMAALAWLALYQTLDPAAEALVAA
                     LPVDRASHLGGALQFFFYDTPKVLMLLTAVVFVMGMINSYFTPERTRALLAGRSEGVA
                     NVMAASLGIVTPFCSCSAVPLFIGFVQAGVPLGVTFSFLISAPMVNEVALTLLFGMFG
                     WKVALLYLGLGLSVAIVSGWIIGRLKMEAYLEDWVRDMPRMSADYVATGMKLGDRIEG
                     GFQAVKEIVGKVWPYILGGIAIGALIHGYVPQDFMASFMGKEAWWSVPLAVVMGVPMY
                     TNAAGVIPIVQALLDKGAALGTVLAFMMSVIALSLPEMIILRKVLKVRLIATFVGVVA
                     TGILLVGFVFNAVL"
     gene            2943..3176
                     /locus_tag="PLB25_14905"
     CDS             2943..3176
                     /locus_tag="PLB25_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HOE42898.1"
                     /translation="MDIKILGTGCANCKNTVNLIDQVAKAKGVSVTLSKVEELRDIMG
                     YGVMSTPGVVINGKVVHAGGVPSRDKVEQWLSA"
     gene            3192..3425
                     /locus_tag="PLB25_14910"
     CDS             3192..3425
                     /locus_tag="PLB25_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HOE42899.1"
                     /translation="MHPQKKFHLVFSFVMGAMMISLMTFVITWVNVGLGADFLSRWMK
                     AFGIAYLVGVPVIFFLAPVARKLTGHILGVKPA"
     gene            3644..3988
                     /locus_tag="PLB25_14915"
     CDS             3644..3988
                     /locus_tag="PLB25_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE42900.1"
                     /translation="MEKSTATSVFESLSSGVRLDIFRLLVKAGPAGLVVGEIGSALEV
                     PPTNLSFHLKALTHAQLVAVAQEGRYQRYRANLTLMQELIAYLTAECCSGHPEQCAEL
                     QVPLVTGMPLSN"
     gene            4026..4409
                     /gene="arsD"
                     /locus_tag="PLB25_14920"
     CDS             4026..4409
                     /gene="arsD"
                     /locus_tag="PLB25_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter metallochaperone
                     ArsD"
                     /protein_id="HOE42901.1"
                     /translation="MKNVQVFDPALCCSSGVCGTDVDQKLVDFSADVDWAKQQGAQIE
                     RFNLAQQPMAFAQSTAVRGLLERSGESALPITLVDGEVAFAGRYPTRDDLARWIGTAA
                     SASSDAAPSGAATANSCCSGGKCCG"
     gene            4406..>4703
                     /locus_tag="PLB25_14925"
     CDS             4406..>4703
                     /locus_tag="PLB25_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HOE42902.1"
                     /translation="MKFLDQPPRFLFFTGKGGVGKTSIACATSIQLAATGKRVLLVST
                     DPASNVGQVFGISIGNQVTPVTEVANLSALEIDPQAAAQAYRDRIVGPVRGLLPE"
BASE COUNT          941 a         1289 c         1401 g         1072 t
ORIGIN      
        1 agacgccaca gtaaagggtg gctgttttac gcattggcgg acattgccag ccgacttgtt
       61 tgacgaggtg cggcatggcg attggttggt tgacaatcct gaaaatggtg cccttgggcg
      121 acgtgatcga aaatgcccca aggtgcccac cggtgccaaa aagctctgga gtcgggtgcc
      181 aaaaacctgc tggtgccaat gtcaaagtgg ccgctggcgc ggtgactgag tggcccgtga
      241 tgctgctgca gttgcgggca caggtgacca tgaagaaaaa acgcaagcag cagcgtgggt
      301 tgcgcctttg cgagtacacc cctgcgttgt ctgataaggt ggccgtcgaa agttatgagt
      361 tcctgcaggt gatcagctcc agtcttctgg tgcgccattg cggttgcatt gaacgccacc
      421 aagcaaaaaa gccaagtcca atgtcaaagc gcttgaatag tggcacttct ctgtagagtc
      481 attctgattg gcactgacaa tgcgatgcct gaagagcacc tgtagtcata ccggtgccgt
      541 ttggctttga gtcttgacag tgtggtcacc agtccacccg gtgttttgcc atcacgtcat
      601 agcgtagtgg aagacgcagg gcgcgggcat gttcgcaagg catggaatca ctgtgcgggc
      661 agcgtcggca aagccgctga tgtgctgcgc atttccggta aaactttatg ggagaaaatg
      721 aaacggctgg agattggtgc atacgccatg tcaggcagac cagcaatttt caagctactt
      781 gtttaatagc tatcaccgca atccaatcaa gggttagaag ccttttcatc tattttgaca
      841 accccagcct gtctgaccac tgcctggaca attcacgccc ctgagttgcg caaatggcta
      901 tgccgtcgca ccagcaaccc ggccctggcg gacgatttgc tgcaagacct gttcttgaag
      961 gcgctgcgtc aaggtagccg cttttgtgaa gttcgtaacc cccgtgcctg gctgtttgaa
     1021 gtggcgcgca acctgctggc cgaccaatgg cgcgtggccc aacatctggt ggcgttaccg
     1081 gaagacctgg cggtagacac agacgatgtc gacaccgttg atcacttgac ggcttgcctg
     1141 ccacgggtat tggcagagtt aagtgctgat gaccgcgagg ccatcacgct gtgtgatttg
     1201 cagggcatgg cgcaggctga ttttgccaag gccaagggtt tgagcctgag cgcagccaaa
     1261 tccaggctgc agcgctcacg cgtcaagttg cgcactcatt tgagtcaggc ctgtcaggtg
     1321 caaatcaacg cggatggaac ggtcgcagac tttgtgccca ggccaccttt ggcggattga
     1381 tggctcgacg atgctacata aagagtagct tgcagcgctt attgaacaag ggctagcggc
     1441 ccatttctta taatttttca aaaaacttgc gtcttttgct gtgttggtcg gtctccattg
     1501 gcatgacaac atttaccgca ccacttagag cctggccctt gcgtcagccc gttttttttc
     1561 tgcttatggc cgctttggcc tggctggcgc tgtaccaaac gcttgaccca gccgctgaag
     1621 cactggtcgc cgccttgccg gtagaccgcg ccagccactt gggcggtgcg ctgcaattct
     1681 tcttttacga cacgcccaaa gtgctgatgc tgctcacagc cgtggtgttt gtcatgggca
     1741 tgatcaacag ctacttcacc cctgagcgca cccgcgccct gctggccgga cgcagtgagg
     1801 gcgtggccaa tgtgatggcg gcatccttgg gtatcgtgac accgttttgc tcatgttcag
     1861 cggtgcctct cttcatcggc tttgtgcaag caggtgtgcc gctgggggtc acattctcgt
     1921 ttttgatttc ggcaccgatg gtcaacgaag tggcgctgac gctgctgttt ggcatgtttg
     1981 gctggaaggt ggcgctgctg tacctggggt tgggcttgag cgtggcgatt gtgtcgggct
     2041 ggatcattgg ccgcctgaaa atggaagcct acctggagga ctgggtgcgc gacatgccca
     2101 ggatgtcggc tgactatgtt gccaccggca tgaagctggg tgaccgcatt gaaggtggct
     2161 ttcaggccgt caaggaaatt gtcggcaagg tctggcccta cattctgggc ggcattgcca
     2221 ttggcgccct gattcacggc tatgtgccgc aagatttcat ggccagcttc atgggcaaag
     2281 aagcctggtg gagcgtgccg ctggcggtcg tcatgggcgt gccgatgtac accaacgcgg
     2341 ctggcgtgat cccgatcgtg caggctttgc tggacaaagg tgccgcgctg ggcacggtgc
     2401 tggctttcat gatgagcgtg attgccctgt cactgcctga gatgatcatc cttcgcaagg
     2461 tgctcaaagt gcggctgatc gccacttttg tcggggtcgt ggccactggc atcttgctgg
     2521 tggggtttgt gtttaacgcg gtgctgtgac cggcacggca tcctgatctg ctgtctttca
     2581 acccatcaaa ctcaaaccaa ggcccttatg aaaccgccca ttcctgaagc gccgacacgc
     2641 tggccagcgt gctgctggtc atggtcaggc gaccgacctg gccaccagca gccgcatgtt
     2701 cgcgacccgc caccggcacc atgcagccgc atggaaaatg cggtgggcgt tggcgtggcc
     2761 attggcgctg cgatcaagcc tagtgacacc aaaccctgtg ccaaacgcaa agccgccgac
     2821 ctgtggatgc cgcaactggc ccatcaggct gcgctgcggc caaaaaccca aataatgtca
     2881 gtgaaatatg gtcgtagccc ttgctgtgat tgcgctgaca gctccttttt tgaaagcaaa
     2941 taatggacat caaaatactc ggcaccggtt gtgccaactg taaaaacacg gtcaacctga
     3001 ttgatcaggt tgccaaagcc aaaggcgtgt cagtgaccct gagcaaggtt gaagaactgc
     3061 gcgacatcat gggctacggc gtgatgagca cgcccggtgt ggtgatcaac ggcaaggtgg
     3121 ttcacgcggg tggtgtgccc agccgtgaca aggttgaaca gtggctcagc gcttgaacca
     3181 ggggttttgc catgcatccg caaaaaaagt tccacttggt gttttccttc gtcatggggg
     3241 ccatgatgat ttcgctgatg acctttgtca ttacctgggt caatgtcggc ttgggggctg
     3301 actttttgtc gcgctggatg aaggcctttg gcatcgccta cctggtgggt gtgccggtga
     3361 tcttcttcct ggcaccggtg gcacgcaaac tcaccgggca cattctgggg gtcaagccag
     3421 cctaaattca gccaagggca ccatcatggc tggcagtcgc gctgtatccc catcaaattc
     3481 aaatagacgc tgactgtttg tgcaggagtc gcgccctcgc gacgaaggtc gtccaattcc
     3541 ggctcgtccg gcttaggttg attcagaaat ggatggacgt gtctgccagc cacacaacat
     3601 ttcaaatgtt caatagtttg attattgttg tatcattgct gcaatggaaa aatcaactgc
     3661 aacctccgtg tttgaatccc tctcgtcggg cgtccgactg gacatctttc gactgctggt
     3721 caaggccggg cctgccggct tggtggtggg tgaaattggc agcgctttgg aggtgccgcc
     3781 caccaacctg tcgtttcacc tcaaggcact cacccacgcg caactggtgg cggtggcgca
     3841 agaggggcgc taccagcgct accgggccaa cctgacgctg atgcaggaac tgattgccta
     3901 tttgacggcc gagtgttgtt caggtcaccc cgagcaatgt gccgagctgc aggtgccgct
     3961 ggtcactggc atgcccctga gcaactgacc aaccaagcac ttaaacaacc caagcgagat
     4021 tcatcatgaa aaatgttcaa gtctttgacc cggccctgtg ctgcagcagc ggcgtatgcg
     4081 gtaccgatgt tgaccaaaag ctggtcgatt tctctgctga tgtggattgg gccaaacaac
     4141 aaggcgcgca aatcgagcgc tttaacctcg cccagcagcc catggctttt gcacaaagca
     4201 cagcggtgcg cggcttgctg gagcgctcgg gcgaatctgc cttgcccatc accctggtgg
     4261 acggtgaagt ggcttttgcc gggcgttacc cgactcgcga cgacctggcg cgctggattg
     4321 gcacggcggc ttcggccagt tccgatgccg caccaagcgg tgctgccaca gccaacagtt
     4381 gctgcagcgg cggcaagtgt tgcggatgaa gttccttgac cagcccccgc gctttctgtt
     4441 cttcacgggc aagggcggtg tcggtaaaac ctcgattgcg tgcgccacgt cgattcaact
     4501 ggccgccaca ggcaaacgtg tgctgctggt cagcactgac ccggcctcca acgtggggca
     4561 ggtttttggc atcagcattg gcaaccaggt gacaccggtg accgaggtgg cgaacctgag
     4621 cgcgctggag attgacccgc aagccgctgc ccaggcttac cgcgaccgca tcgtcggacc
     4681 ggtgcgtggc ctgttacctg aga
//
LOCUS       DAOSLL010000373         4690 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16240_length_4690_cov_5.116505, whole genome shotgun sequence.
ACCESSION   DAOSLL010000373 DAOSLL010000000
VERSION     DAOSLL010000373.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16240_length_4690_cov_5.116505"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="PLB25_14930"
     CDS             complement(<1..1028)
                     /locus_tag="PLB25_14930"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HOE42903.1"
                     /translation="MQSTFDSTAPVLTVDAVGKTYITPVLQGVNLNLHPGEVLALTGE
                     NGAGKSTLAKIICGLESPSQGAMTLAGKVFLPSSRRNAESFGVRMVMQELSLIPTLTV
                     AENLLLVEIPNRGRWQGNWIDRARLNALAVQKLAKVGMQAINPETLVSQLGIGQQQMV
                     EIARNLQDDTRVLILDEPTAMLTPSETSHLFEQIARLKSQGVAIIYVSHRLEELKTIA
                     DTLAVLRDGNLVKVCPVDSVTEDDIIQMMVGHSVSQDLERPPRPRGHLLLNAKDIGRG
                     HLVRHISLDLYAGEVFGLAGLVGSGRTEVVRLLFGADQNEHGEITVFENSPGQPIRTK
                     AKKWSSPLA"
     gene            complement(1172..2125)
                     /locus_tag="PLB25_14935"
     CDS             complement(1172..2125)
                     /locus_tag="PLB25_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HOE42904.1"
                     /translation="MKFTRRAVQATIALGLLGGLFATPSHAQDKPKVALVMKSLANEF
                     FQTMEAGAQAHNKSHASDYTLVSNGIKNETDTAAQIRMVEQMIAQKANAIVIAPADSK
                     ALVPVLKTAIDKGILVVNIDNQLDPAVQKEKGIQIPFAGPDNRTGAKLVGDYLAKQLK
                     AGDKVGILEGVSTTFNAQQRTLGYQDAMKAAGVAVVGVQSGDWEINKGNTVASGMLRE
                     HPDLKALLAGNDNMAIGAVAAVKAAGKVGKVMVVGYDNIDAVKPMLKDGRMLATADQF
                     GAKQAVFGIEIALKALKEKKKQAEMPAAIQTDVVLVTKDSK"
     gene            2334..3761
                     /locus_tag="PLB25_14940"
     CDS             2334..3761
                     /locus_tag="PLB25_14940"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HOE42905.1"
                     /translation="MHPNTTLKFADGISVVVPDSLNLITPYVIREQGDWFEDEIKFVR
                     LLLKPDQKAIDIGANYGLFTLSMAKIVGPGGGIWAFEPASSTSAFLSESLAINGFSHV
                     VLDQRALSDHSGTAQLSLHDNSELNELVRDGDSLGSSETVTLISLDDAMREHAWSDIE
                     FVKIDAEGEEGAIIQGGRTFFATQSPLIQYEVKAGAAIHLELIQAFADVGYASYRLVP
                     GLDALVPFDPQVVADGFLLNLFCCKPDRAATLAAQGRLVLADDLQTALHTQRVDIAQH
                     GRQANSAYGWQNQLTRLPYGTHLAGSWQHTVSQGQSGEVEKALALHAAAHDKELPIAE
                     RFLALQNSLEILTSVCSGQPAFLRLLSLARVAREFGHRMVAVKALGDLCQLAMKHQSV
                     NPGEPFLASSERFDSLDPKDSMGNWALCSILEELERNSSFSSFYSGQAAKQRLEIIRN
                     LGFGSPEMARRLALVERRFSGATNR"
     gene            complement(3816..>4690)
                     /locus_tag="PLB25_14945"
     CDS             complement(3816..>4690)
                     /locus_tag="PLB25_14945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE42906.1"
                     /translation="LDVMMPEVDGFETFRRLAAQPKLQDIPVMFVTALTDTESEVTGL
                     SLGAADYITKPINVTIARHRIRNLLEREYLRKEVGVQRDLLHEEIARRVKSEERVRQL
                     ALHDALTGLPNRRMLADRLGQTMAASNRSGLYGALMFLDLDNFKPLNDAYGHEVGDLL
                     LIEVARRLCDCVREMDTVVRLGGDEFVVMIRELDADKALSVEQAAGVAEKIRVKIAKP
                     YQMSITRPDQTGCSVEHHCSVSVGVVVFIDHQASQADIMKLADAAMYQAKDAGRNTIR
                     FSDLPKTGAANNGP"
BASE COUNT         1004 a         1347 c         1228 g         1111 t
ORIGIN      
        1 gccagtggtg aagaccattt cttggctttt gttcgtatcg gttggcccgg tgagttctca
       61 aaaaccgtga tctcgccgtg ttcgttctga tcggcgccaa agagcaacct gacgacttct
      121 gtgcgtccac tgcccacaag tcccgccaga ccgaacacct caccggcata cagatcaagg
      181 ctgatgtgcc tgaccaagtg gccccggcca atgtctttgg cattgagcag caggtgaccg
      241 cgtggccgag gtggtcgttc aaggtcttgt gaaacagaat ggccgaccat catttgaatg
      301 atgtcatcct cggtgacgct gtcaaccggg cagaccttga ccaagttgcc gtcacgcaag
      361 accgccaagg tgtcggcgat ggttttcaat tcttccagcc ggtgcgacac gtaaatgatg
      421 gccaccccct gagatttcag tcgggcgatc tgctcaaaca aatggctggt ttcgctcggt
      481 gtgagcatgg cggttggctc gtcgagaatc aacacacggg tatcgtcctg caggttgcgg
      541 gcgatttcga ccatctgctg ctgaccaatc cccagttgcg ataccagagt ttccgggttg
      601 atggcctgca tgccgacttt ggcaagtttt tgcactgcca atgcattgag gcgggcacgg
      661 tcaatccagt tgccctgcca cctgccacgg ttcggaattt ccaccaaaag caggttctcc
      721 gccaccgtca aggtgggtat caggctgagt tcctgcatga ccatccgcac gccaaagctc
      781 tcggcgttgc gccgtgaact cggtaaaaac actttccccg caagcgtcat tgcgccctgg
      841 ctgggtgact caagcccaca gatgattttt gccagcgtac ttttgccagc gccattttca
      901 ccggtcaagg caagcacctc accaggatgc aagttcaggt taacaccctg caacactggc
      961 gtgatgtagg ttttgcctac ggcatcaacc gtcagcactg gcgcagtaga gtcaaaggtg
     1021 gactgcatgg catggatcta ttgagttttg aaagtgcttg ctggtcagcc gaccggaaac
     1081 ctttgtcagc cgacgacaca gcgcgtggcg agcaggtgtg atgtttcaaa ccgacaaccc
     1141 gctgccacac tgctgtgcat cagaacccgc gttacttgct gtctttggtc accagcacca
     1201 cgtcggtctg gatggcggct ggcatttcag cttgcttttt cttctccttc agcgctttca
     1261 aggcgatttc aatgccaaat acagcctgtt ttgcaccaaa ttgatcggct gttgcgagca
     1321 tgcgaccgtc tttgagcatg ggcttgacgg catcaatgtt gtcgtaaccc accaccatca
     1381 ccttgccaac tttgcccgca gccttcaccg cagcaacggc accgatggcc atgttgtcgt
     1441 tgccggccaa cagtgccttc agatcggggt gctcacgcaa catcccggaa gccactgtat
     1501 tgcctttgtt gatttcccag tcgccagact gcacgccaac cactgccaca ccggcagcct
     1561 tcatggcatc ttggtacccc aaggtgcgct gctgggcatt gaaggttgtt gatacgcctt
     1621 caaggatgcc aaccttgtcg cccgctttaa gttgtttggc cagatagtca ccaaccagtt
     1681 tggctccggt gcggttgtca ggacccgcaa atggaatctg gatgcctttc tctttttgaa
     1741 ctgcaggatc caactggtta tcgatattga ccaccagaat gcctttgtcg attgccgttt
     1801 tcagcaccgg caccagcgcc ttggaatcag caggtgcgat cacgatggca ttggcttttt
     1861 gcgcaatcat ttgctcaacc atgcggatct gggcggctgt atcggtctcg ttcttgatgc
     1921 cgtttgaaac caatgtgtag tcagacgcat gagacttgtt gtgcgcctgg gcacctgcct
     1981 ccatggtttg aaagaactca tttgccaacg atttcatgac aagagcaact tttggtttgt
     2041 cttgtgcatg gcttggtgtg gcaaacaggc cacccagcaa acctagagca atagtggctt
     2101 gtacagcgcg acgggtaaat ttcatgagag gaactcctta aatttgtgac tggcaattgg
     2161 tctttgaccc aatgaaggcg acaaagcttg tttggcatgc agctctgcct tcttcgaggc
     2221 agattctaca tccgcaaacg attgcgcaaa cgattgcttt attaggggaa accctctctt
     2281 ttaaccggta aaattcaaag tggctcttca gtccaccacg gttctcagtc taaatgcacc
     2341 caaataccac tctgaaattc gccgatggca tcagcgtcgt cgtacctgac tcgttgaacc
     2401 tgattacgcc ttatgtgatt cgtgagcaag gcgactggtt tgaagatgaa atcaagtttg
     2461 tccgcttgtt actcaagcca gaccagaagg cgatcgacat cggtgcaaac tatggtctgt
     2521 tcaccttgtc gatggcaaag attgttgggc cgggtggcgg catttgggcc ttcgagcccg
     2581 catcgtccac gtctgctttt ctttccgaaa gcctggccat caacgggttc agtcatgtcg
     2641 tgctcgacca gcgcgcccta tctgatcact caggcactgc ccaactgtcg ctccatgaca
     2701 actccgaact gaacgaactt gtccgcgatg gcgactcatt gggatcatcc gaaactgtta
     2761 ccttgatttc gctggacgat gcgatgcgag agcatgcatg gagcgacatc gaatttgtga
     2821 agatcgatgc cgagggagag gaaggcgcca tcatccaggg tggacggacc ttctttgcta
     2881 cgcaatctcc gctcattcag tatgaagtca aggcaggcgc tgcaatccat ctggaattga
     2941 tacaggcttt tgcggacgtc ggatatgcgt cttaccggtt ggtacctgga cttgatgccc
     3001 tggtgccctt tgacccccag gtggtcgcag acggatttct gctcaacctg ttttgctgca
     3061 agcctgatcg tgcagcaacg cttgctgcgc aaggccgcct tgttttggcc gacgatttgc
     3121 aaacagcgtt gcacacccag cgtgtggata ttgcgcagca cggtcgccaa gccaactcag
     3181 cgtatggctg gcagaaccaa ctgacacggc taccttatgg cacgcatctg gcagggagct
     3241 ggcagcacac tgtcagtcag gggcaaagcg gtgaagttga aaaagcactc gccctgcacg
     3301 cagcagcaca cgataaagaa ttgccgatag ccgagcgctt tcttgccttg caaaacagtc
     3361 tcgaaatcct gacatcagtc tgcagcggac agcccgcatt ccttcgcctc cttagtctgg
     3421 cccgggttgc acgggaattc ggacaccgca tggtcgccgt taaggcgctc ggtgatttgt
     3481 gccaacttgc catgaagcat cagagcgtca atccgggcga acccttttta gccagcagcg
     3541 agcgcttcga cagtcttgac cccaaagact ccatgggcaa ctgggcgctt tgctcaatac
     3601 tggaagaact ggaaaggaac tcttcgttct catccttcta ttctgggcag gcggcaaagc
     3661 aacgcctgga aatcatccgc aacctcggct ttggtagccc cgagatggct cgacgcctgg
     3721 cccttgtcga aaggcgcttt tcgggcgcaa ccaaccgata gcttttggca gcctggttca
     3781 gcgttgcatt gcctgctgaa accggcacaa ggcagtcagg ggccgttatt ggcagcgcca
     3841 gttttgggca aatcggaaaa ccgaatcgtg ttgcgcccgg catccttggc ctggtacatc
     3901 gccgcatcag ccaatttcat gatatcggcc tgagaggcct ggtggtcgat aaacaccacc
     3961 acccccacgc tgacgctgca atgatgctcc accgagcacc cggtttgatc tggccgcgtt
     4021 atgctcattt ggtagggttt tgcgattttg acgcggatct tctcggccac accggcagct
     4081 tgttcaaccg acaaagcctt gtcggcatca agttccctga tcatcaccac gaactcgtcg
     4141 ccacccaatc gaaccactgt gtccatttca cgcacacagt cgcacaagcg ccgggcgacc
     4201 tcgatcaaca gcaagtcgcc aacctcatgc ccgtaggcat cgttgagcgg cttgaagttg
     4261 tcgagatcga gaaacatcaa cgcgccgtac agcccactgc ggttactggc cgccatggtt
     4321 tggccaaggc ggtctgccag catgcgacgg tttggcagtc cagtcagcgc gtcgtgcaag
     4381 gcaagttgac gcacccgctc ctcggacttc acacgccggg caatttcctc atgaagcaaa
     4441 tctcgctgta ccccgacttc cttgcgcaaa tactcgcgct ccaacaggtt gcgaatgcga
     4501 tggcgtgcaa tggttacatt gatcggtttg gtgatgtagt ctgctgcgcc cagcgacaag
     4561 ccagtcactt cggactccgt gtcagttaac gccgtcacaa acatcaccgg gatgtcctga
     4621 agcttcggct gcgccgccag ccgcctgaag gtttcaaaac catcaacctc cggcatcatc
     4681 acatcgagca
//
LOCUS       DAOSLL010000374         4684 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16262_length_4684_cov_3.913372, whole genome shotgun sequence.
ACCESSION   DAOSLL010000374 DAOSLL010000000
VERSION     DAOSLL010000374.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16262_length_4684_cov_3.913372"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2588
                     /locus_tag="PLB25_14950"
     CDS             <1..2588
                     /locus_tag="PLB25_14950"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE42907.1"
                     /translation="FEFQKDGVKGAVQKINTHNGCILADSVGLGKTYSALAVIKYFEL
                     RNHRVLVLCPKKLRENWTVYLGSNITELNPFVRDRFSYMVLSHTDLSRESGKAGDVDL
                     AAINWGNFDLVVIDESHNFRNNVKGKRDEEGNVIKQSRYERLMQDIIQAGVKTKVMLL
                     SATPVNNDLKDLRNQLYLVSEGRDHALLPTTGIASIKQTLDSAQKTFATWAKKPVTGT
                     GDRDPKDLMDALPAGFFTLLDELTIARSRKHIQKYYRASMAQIGKFPKRAKPISKYSE
                     IDSKGRFPSYDKLNEEIEKYQLALFKPSVYVLDQFKDQYQGEASVRNFSQENREKFLI
                     GMMKVNFLKRLESSVKSFAITMERTVSKIEDLEERLKAYQKLRDGKAQDLQATLFLME
                     AEEDDELREAFEVGTLKYRLEHMRVDDWLTDLATDKRQLSLLAESAGGVTPERDAKLT
                     ELKAIIKTKVTSPSTNNRGEANRKIIVFCAFADTAAYLYAQIEPWASTTLRRHIALVS
                     GGTFPNRTTFGAPEFNQILTNFAPRAKQRAKMSKMLQNGEIDILIATDCISEGQNLQD
                     CDLLVNYDIHWNPVRIIQRFGRIDRIGSPNDTIQLVNFWPTPDLNKYINLKNRVEARM
                     ALVDISATNEDNLLTNAELEELIHQDLKYRDKQLMRLKDEVLDLEDFNESVALNEFSL
                     DDFRMDLSNYVQANRTKLQDAPFGLYGVVPASTGQASVQPGVIYCLRQQQHKAAQGAE
                     ATNQTVNPLSPYFLVYIHADGAVRYNFAAPKQVLEIFRALCQGKTEPYADLCKLFDEQ
                     TRHGQDMSAYSALLDKTVAAIVAQFTRKNTANLFTGRGGKLMDASKAVKSCDDFELIT
                     WLVIK"
     gene            2622..2876
                     /locus_tag="PLB25_14955"
     CDS             2622..2876
                     /locus_tag="PLB25_14955"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOE42908.1"
                     /translation="MIATAKIFTTGNSQAIRLPKAFRLDTEEVWISKSSDGILTIQPK
                     PKKDDLEAFLAELRSMPPSEEFLQPRDDDPRPSPFDGWDE"
     gene            2876..3280
                     /locus_tag="PLB25_14960"
     CDS             2876..3280
                     /locus_tag="PLB25_14960"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOE42909.1"
                     /translation="MRYMLDTNIVSYYLRRSSPTLEERINDGLKRRTIAISVITRAEL
                     RFGQMGMVTEDRRRALIDSFLLRLPSVEWSIEAADQYGALKHALKALGTPIGDMDTLI
                     AAHALAEKLILVTHNTRHFERVPGLKLEDWMV"
     gene            3367..>4684
                     /locus_tag="PLB25_14965"
     CDS             3367..>4684
                     /locus_tag="PLB25_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42910.1"
                     /translation="MFTCLGYKSDRTFPVDSVDAFCQQFDPSGLLAHPRAQKSNWKSI
                     HLLFQLTDEELSRNATLFKDTTVRASLLQSYVFFAVELQSGDYARGKLAAITRQINRI
                     FPMPVMVLFKVGSNLSIAVINRRQNKRDESKDVLGKVTLIQNISTVKLHPGHLDILAS
                     FSTSELVARKKPIGSFDQLHAAWEDVFNVELLNKRFYEELSNWYFWARQQVSFPSDLD
                     DKDTKRTEKEKTDTRNATSVIRLLTRLMFCWFLKEKNLIPDHLFDQDAITSALNSVGD
                     EESTYYHAILQNLFFATLNQPMNADGEPVRQFAADGAFYENMNQHGIKTLYRYKKCFS
                     QPDQALALFEDIPFLNGGLFACLDTEDDTGKVQYADGFSRNAKKQPIVPNRLFFAAPQ
                     IVDLSEDFGDAKKKKASVRGLLHILNGYKFTIAESTPVEQEIALDPE"
BASE COUNT         1212 a         1344 c         1153 g          975 t
ORIGIN      
        1 tttttgaatt tcagaaggac ggtgtcaaag gtgccgtgca gaaaatcaac acccacaacg
       61 gctgcatcct ggccgacagc gtgggtttgg gtaaaaccta ttcggcacta gccgtcatca
      121 aatactttga actgcgcaac caccgtgtgc tggtgctatg ccccaagaag ctgcgcgaga
      181 actggacggt ttatctcggc agcaacataa ccgaactcaa ccccttcgtg cgcgaccggt
      241 tcagctatat ggtcttgtcc cacaccgacc tgtctcgtga atctggcaaa gctggtgacg
      301 tggacctggc ggccatcaac tggggcaact tcgatttggt agtgattgac gaatcacaca
      361 actttcgcaa caacgtcaaa ggtaaacgcg atgaagaagg caacgtcatc aagcagagtc
      421 gctacgagcg cttaatgcaa gacatcatcc aggctggtgt caaaaccaag gtcatgttgc
      481 tgtctgccac gccagtcaac aacgacctga aagacctgcg caatcagctc tacctggtgt
      541 ctgaggggcg cgaccacgca ctgttgccta ccaccggcat tgccagcatc aagcaaacac
      601 tggactcagc ccaaaaaacc tttgccacat gggcaaagaa gcccgttaca ggcactggtg
      661 atcgcgaccc caaagacctg atggacgcgc tacccgcagg cttcttcacc ttgctagacg
      721 agctgaccat tgcccgctcg cgcaaacaca ttcaaaaata ctaccgcgcc tcgatggcgc
      781 aaattggcaa attccccaag cgggccaagc cgatttccaa gtattcagaa atagacagca
      841 agggccgctt cccaagctac gacaagttga acgaggagat tgaaaagtat cagctcgcgc
      901 tgttcaagcc atcggtttat gtccttgatc agtttaaaga tcagtatcag ggcgaagcca
      961 gcgtgcgcaa cttcagccag gagaaccgtg agaagttttt gatcggcatg atgaaagtga
     1021 acttcttgaa gcggctggaa agctcggtca aatcatttgc catcaccatg gagcgcaccg
     1081 tcagcaagat tgaagacttg gaagagcgtc tgaaagccta tcaaaagctg cgtgacggta
     1141 aggcacaaga cctgcaagcc accctgtttt tgatggaggc cgaagaagac gacgagttgc
     1201 gtgaggcgtt tgaagtcggc accctcaaat accgtttgga acacatgcgc gtggacgatt
     1261 ggctgaccga tctggctact gacaagcgtc agttatccct gctggcggaa agcgcgggcg
     1321 gtgttacgcc cgagcgcgat gccaagctga cagaactcaa agccatcatc aaaaccaaag
     1381 tcacaagccc cagcacgaac aaccggggcg aggccaaccg caagatcatc gtgttttgcg
     1441 cctttgctga caccgccgcc tatttgtatg cacaaatcga accgtgggcc agcacaaccc
     1501 tgcgcaggca cattgcgctg gtctctggcg gtaccttccc caatcgcacc acgttcggtg
     1561 cgcccgagtt caaccagata ctgaccaact ttgcaccccg cgccaagcag cgcgccaaga
     1621 tgtccaaaat gctgcaaaac ggtgaaattg acatactgat tgccaccgat tgcatctctg
     1681 aaggccagaa cctgcaagac tgcgacctgc tggtcaacta cgacattcac tggaacccgg
     1741 tgcgcatcat ccagcgcttt ggccgtatcg accgtatagg cagcccaaac gacaccatcc
     1801 agctcgtcaa cttctggccc acgccagacc tgaacaaata catcaacctc aaaaaccggg
     1861 tggaggcacg catggcattg gtggacattt ctgccaccaa cgaggacaac ctgctgacca
     1921 atgcagaact ggaagagctg attcaccaag acctgaagta ccgcgacaaa cagctcatgc
     1981 gcctgaaaga tgaggtgctg gacctggaag acttcaacga gtccgttgcg ctgaacgaat
     2041 tcagcctgga cgatttccgc atggacctga gcaactacgt gcaagccaac cgcaccaagt
     2101 tgcaggatgc gccctttggg ttgtatggtg tcgtacccgc cagcaccggg caggcgtcag
     2161 tccagcccgg tgtcatctac tgcctgcgcc agcaacaaca caaggcagcg cagggcgctg
     2221 aagcaactaa ccaaacggtc aaccctttga gcccgtattt cttggtctac atccatgccg
     2281 acggcgcagt gcgctataac tttgccgcgc ccaaacaagt gctggaaatc ttccgcgccc
     2341 tgtgccaggg caagaccgag ccctatgccg acctgtgcaa gctatttgac gagcaaactc
     2401 gccatgggca agacatgagc gcgtattccg ccttgctgga caaaaccgta gcggccatcg
     2461 tggcacagtt cacccgcaaa aacacagcca acctgttcac aggccgtggc ggcaaactga
     2521 tggatgcgag caaagcggtc aagagctgcg atgactttga attgataacc tggttggtga
     2581 taaagtagat acgttttcat atctacttac aggggtaaac catgatagcc accgccaaaa
     2641 ttttcaccac gggcaacagc caggctattc gcttgcccaa ggcatttcgg ttggataccg
     2701 aagaggtttg gatcagcaaa tccagcgacg gcattctcac cattcagccc aaacccaaaa
     2761 aagatgactt ggaggcattt ctggccgagt tgcggtctat gccaccaagc gaagagtttt
     2821 tgcagcctcg tgacgatgac ccccgcccga gcccttttga tgggtgggac gagtaatgcg
     2881 ctacatgctt gacaccaaca tcgtcagcta ttacctgcga cgttcatcac ccacgctaga
     2941 agagcgcatt aatgatgggc tgaagcgccg caccatcgcc atttccgtca tcacccgcgc
     3001 tgagctacgg ttcggccaaa tggggatggt cacggaagac cgccgccgcg ctctgataga
     3061 cagttttttg ctgcgacttc ccagcgttga gtggagcatt gaagcagctg accaatatgg
     3121 ggcactgaag cacgccctta aagcactagg cacccccatc ggcgatatgg acaccctgat
     3181 cgccgcgcat gccctggccg aaaaactcat tttggtcacc cacaacaccc gccattttga
     3241 aagagtgccg ggcctgaagc ttgaagattg gatggtttga aagtggctga tttccatcaa
     3301 aaactaacaa ttcaaactgc tatacacgcc ttcacatcta agccgttgca agatgctgca
     3361 gtcgagttgt tcacttgtct tggctacaaa tctgaccgca cttttcccgt tgattcggta
     3421 gacgctttct gtcagcagtt tgatcccagc ggactgctcg ctcatccgcg tgcgcaaaag
     3481 tctaattgga agtccatcca cttgctgttc cagctcaccg acgaagagtt gtcacgcaac
     3541 gccacccttt ttaaagatac gactgtcaga gcgtctctgc tccagtccta tgtgttcttt
     3601 gctgtggagc ttcagtcggg tgattacgca cgaggcaaat tggccgctat cacccgccag
     3661 atcaatcgca tctttcccat gccagtcatg gtgttgttca aagtgggcag caatttgtcc
     3721 attgccgtca tcaaccgcag gcagaacaag cgtgatgaaa gcaaagatgt cttgggcaag
     3781 gtcacgctga tccaaaacat ctctacagtc aaactacatc ctggtcacct ggacattctg
     3841 gcttccttct cgaccagcga actggtcgcc cgaaagaagc ccattgggag tttcgaccag
     3901 cttcatgccg cgtgggaaga cgtgttcaac gtcgagttgc tcaacaagcg cttttatgag
     3961 gaactgtcca actggtattt ttgggcacgc cagcaagtca gttttccgtc tgacctcgac
     4021 gataaggaca ccaaacgcac agaaaaagaa aaaacagaca cccgcaacgc caccagtgtc
     4081 atccgtctgc tgacacggct gatgttctgt tggttcctga aggaaaaaaa cctcatcccc
     4141 gatcaccttt ttgaccaaga tgccatcacc agcgccttga attctgtcgg tgacgaagag
     4201 agcacctatt accacgctat tctgcaaaac ctgttttttg ccacgctcaa ccaacccatg
     4261 aatgccgatg gtgagcctgt caggcagttt gctgcggacg gtgcttttta tgaaaacatg
     4321 aaccagcacg gcatcaaaac cctttatcgc tacaagaaat gtttcagtca gccagatcaa
     4381 gcgttagcgc tgtttgaaga cattccattc ctaaacggcg gcttattcgc ctgccttgac
     4441 accgaagacg acaccggcaa ggtgcagtat gccgacggtt tctcgcgcaa tgccaaaaaa
     4501 caacccatcg tccccaatcg cttgttcttt gccgcgccgc agatcgttga cctaagcgaa
     4561 gactttggcg atgccaagaa gaaaaaggcc agcgtgcgcg gcctgttgca catcctgaac
     4621 ggctacaagt tcaccattgc cgagagcacg ccggtagagc aagaaatcgc cctcgatccc
     4681 gaac
//
LOCUS       DAOSLL010000375         4682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16268_length_4682_cov_4.323968, whole genome shotgun sequence.
ACCESSION   DAOSLL010000375 DAOSLL010000000
VERSION     DAOSLL010000375.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16268_length_4682_cov_4.323968"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1736)
                     /locus_tag="PLB25_14970"
     CDS             complement(<1..1736)
                     /locus_tag="PLB25_14970"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOE42911.1"
                     /translation="MNTKELIDRIFKEPGVKFELTEFENLGKPIHDILSIYPKTASTG
                     KDAGKVKFYLKSFIPFSTGNEEVLVYAEGGKSAPEEIIRQLWVYKLKHQYGYQDDEID
                     LEKGVQFGTEVGTKAADIIVYTNSTKVTPKIIVECKKPKRKDGIEQLKSYMNAKGAPV
                     AVWSNGADSIILYRPYPAQFDDTLFDIPKRGKEPKDVLEAKKTLLQLKREFNFKNIIQ
                     GLEELVLADSGKDEFNEIFKLIFAKIWDEKQADEVRKDKTVEFGQAVNSKTGVPDPDL
                     TYDRINSLFHQACEEWPGIFRPGEDIELARRHLQVCIGSFEGVRLMGSNLRIMDDAFE
                     YLLPTEAKKKKGQFFTPRHVVEMCVRMLNPTRTEYVMDPSCGSGGFLLHAMDWCYPAT
                     DNAQRETRKHKYAAKYLWGIDFEQRAAKTSRALMLIAGDGHTKIFGPDVSSLDPRTWY
                     ENASGQALMHELRQAKLTARKIPDGEMLKDEDKAWEYFDELKFDVILANPPFAGEMKD
                     RKMLVRYELAKPALKRAGDDKSPKEERDVLFIERILKMLKPGGRAAIVLPQGKFNNSS
                     LAFIREWILKKAR"
     gene            2227..3012
                     /locus_tag="PLB25_14975"
     CDS             2227..3012
                     /locus_tag="PLB25_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE42912.1"
                     /translation="MSHLSFGYGERLILDDISLAIPRGKVTALMGASGGGKTTVLRLI
                     GGQIKAQTGQMLFDGQDITPMNQAELYAARRRMGMLFQFGALFADLNVFENVAFPLRE
                     HSDLPEALIRDVVLMKLNAVGLRGARSLMPSDLSGGMARRVALARAVALDPELVMYDE
                     PFAGLDPISLGTAARLIRELNDAMGLTSIFVSHDLEQTFAIADQVIILANGKVATQGT
                     PDEVRHSTDPLVYQYVNALPDGPVHFHYPGPTVEQDFGLELTA"
     gene            3009..3791
                     /gene="mlaE"
                     /locus_tag="PLB25_14980"
     CDS             3009..3791
                     /gene="mlaE"
                     /locus_tag="PLB25_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HOE42913.1"
                     /translation="MSWYQPKDIGFAVRGHLDAMGFAARLFGRLLATPGYALRRNVLV
                     RDQIHFLGNYSLPIITVAGLFVGFVFGLQGYYVLKRYGSAESLGLMVCLSLLRELGPV
                     VSALLFAGRAGTSLTAEIGLMKAGEQISVMEMMAVDPIQRVLAPRFWAGVITMPLLAA
                     MFSATGVIGGWVVGVLMIGVDGGAFWSQIQGGVDVWQDLGNGVVKSLVFGVAVTFIAL
                     LQGYNAQATPEGVAKATTRTVVMASLTVLGLDFILTAMMFTI"
     gene            3828..4325
                     /gene="mlaD"
                     /locus_tag="PLB25_14985"
     CDS             3828..4325
                     /gene="mlaD"
                     /locus_tag="PLB25_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HOE42914.1"
                     /translation="MQRSKNDVWVGLFVLIGAVAILFLALQSANLLSLSFQKSYPVLA
                     KFDNIGGLKPKAAVKSAGVVVGRVEDIVFDDKSFQALVTLALETRYAFPKDSSLKILT
                     SGLLGDQYLGIEAGADDKNLAAGDVISSTQSAVVLENLISQFLYSKAAEVPAPAENGA
                     SKGKP"
     gene            4334..>4682
                     /locus_tag="PLB25_14990"
     CDS             4334..>4682
                     /locus_tag="PLB25_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaA family lipoprotein"
                     /protein_id="HOE42915.1"
                     /translation="MTTVVKSALLRAGLMGLALLMTGCASGPAANRHDPLEPFNRGVF
                     QFNDGLDRAVIKPVATAYRDVLPSPIRTGVGNFFNNLQDAWSFVNNALQLKAEYAGNS
                     LVRFGVNSVFGLAG"
BASE COUNT          859 a         1256 c         1403 g         1164 t
ORIGIN      
        1 cgggccttct tgagaatcca ctcgcggatg aacgccagcg acgaattgtt gaacttgccc
       61 tgcggcagga cgatggcggc acggccgccg ggcttgagca ttttcaggat gcgctcgatg
      121 aagagcacgt cgcgctcttc cttcggtgat ttgtcgtcac cggcgcgttt gagcgcaggc
      181 ttcgccagct cgtagcgcac cagcatcttg cggtccttca tctcaccggc aaaaggtgga
      241 ttggcgagga tcacgtcgaa tttcagttcg tcgaagtatt cccaggcctt gtcttcgtcc
      301 ttgagcattt cgccgtccgg gatttttctg gcggtcagtt tggcttgccg cagctcgtgc
      361 atcagcgcct ggccggaggc gttttcgtac caggtgcgcg ggtccaggct gctgacatcg
      421 ggtccgaaaa ttttggtgtg accatcaccg gcaatcagca tcaacgcgcg cgaggttttg
      481 gcggcgcgct gctcaaagtc aatgccccac aggtatttgg cagcgtactt gtgtttgcgc
      541 gtttcgcgct gggcgttgtc ggtggccggg tagcaccagt ccatggcgtg cagcaagaag
      601 ccgcccgatc cacacgatgg atccatcacg tattcggtgc gcgtcgggtt gagcatgcgt
      661 acgcacattt caacgacgtg gcgcggcgtg aaaaactggc cctttttctt tttggcttcg
      721 gttggcagca ggtattcaaa ggcatcgtcc atgatgcgca ggttcgagcc catcaggcgc
      781 acgccttcaa agctgccaat acacacctga aggtgtcgcc gggccagttc gatgtcttca
      841 ccggggcgga aaatgcctgg ccactcttcg caggcttgat gaaacaggct gttgatgcgg
      901 tcgtaggtca agtccgggtc aggcacgccg gtttttgagt tcactgcctg accaaactcg
      961 accgttttgt ccttgcgcac ctcgtcggcc tgcttttcat cccagatctt ggcgaagatc
     1021 agcttgaaaa tttcgttgaa ttcatccttg ccactgtcgg ccagaaccag ttcttccagt
     1081 ccctgaatga tgtttttgaa attgaattcg cgcttgagtt gcagcagggt ctttttcgcc
     1141 tccagcacat ccttgggctc tttgccacgc ttggggatat caaacaacgt gtcgtcaaac
     1201 tgcgccgggt agggccggta aagaatgatg ctgtcggccc cgttggacca caccgccact
     1261 ggcgcgccct tggcgttcat gtagctttta agctgctcaa tgccgtcctt gcgcttgggc
     1321 tttttgcatt cgacgatgat ttttggcgtg actttggtcg agttggtata gacgatgatg
     1381 tcagccgcct tggtgccgac ctcggtgcca aactgcacgc ccttttccag atcaatctca
     1441 tcgtcctggt agccgtactg gtgcttcagc ttataaaccc acaactgacg aatgatttct
     1501 tcaggggccg acttgccacc ctcggcatac accagcactt cctcattgcc ggtggaaaac
     1561 gggataaagc tcttcaggta gaacttgacc ttgcccgcat ccttgccagt gctggctgtc
     1621 ttggggtaga tcgagaggat gtcgtggatg ggtttgccga ggttttcaaa ctcggtcagc
     1681 tcaaatttga cacctggctc tttgaaaatg cggtcaatca gttctttggt attcatggtt
     1741 tgcgagttgt ctctttgttt ctggccaaag cgcttgtcga tgtcgctgga ttggttttaa
     1801 ttgcctgact gacccaacgc ccagttcagc tatcgcgatg cgcttgcctc cccaatggtt
     1861 ccgagtcatc acctcaatca ggttggtaac acaacggaaa tgatgcactc tacagcaagg
     1921 cacaggggca tccggcggcg tgaacgcaag ttcgcaattg acattgtccg atactacagt
     1981 ttaagtagct gcttgcgcag gatagacagg ggctacagga cgattttgta aaactcagcc
     2041 cggtcttggc agcgcttgat gctcaggcat gacagtcttt aatagggtac ctgaaagctt
     2101 gccatgcaca tggtgttgat gaccccagcc tggcagcgag gcttgccctt caactatgat
     2161 tcagctgccg ggattttccc ggcctccttt tttatggccc caaatttttc agaatccctg
     2221 gttgaaatgt ctcacctgag cttcggctac ggtgagcggc tgattctgga cgacatcagc
     2281 ctggcgatcc cgcgcggcaa ggtcactgcc ttgatggggg cttcaggcgg cggcaaaacc
     2341 accgtgctgc gcctgattgg cgggcagatc aaggcgcaaa ccggccagat gctgtttgac
     2401 ggccaggaca tcacccccat gaaccaggcc gagctgtatg ctgcacgccg tcgcatgggc
     2461 atgctgttcc agtttggtgc gctgtttgcc gacctcaacg tgtttgaaaa cgtggctttt
     2521 ccactgcgcg agcattctga cctgccagag gcgctgattc gtgacgtggt gctgatgaag
     2581 ctcaatgcgg tgggcttgcg cggtgcgcgc tccctgatgc ccagcgactt gtcaggtggc
     2641 atggcgcgcc gggtggcgct ggcccgcgcg gttgcccttg accccgaact ggtgatgtat
     2701 gacgaaccgt ttgccgggct tgacccgatt tcgctgggca cggcggctcg ccttatccgt
     2761 gaactcaacg acgccatggg cctgaccagc atttttgtct cgcatgacct ggagcaaacc
     2821 tttgccatcg ccgatcaggt gatcattctg gccaatggca aagtggccac ccagggcacg
     2881 cccgatgagg tgcgccacag caccgatccg ctggtgtacc agtatgtcaa cgctttgccc
     2941 gacggcccgg tgcattttca ttaccctggc ccgactgttg agcaggattt tggcctggag
     3001 ctgaccgcat gagctggtac cagcccaaag acatcggttt tgccgtgcgc ggtcacctgg
     3061 atgcgatggg ttttgccgcc cggctgtttg gtcgcttgct ggcgacaccg ggctatgcgc
     3121 tcaggcgcaa tgtgctggtg cgcgaccaga tccatttttt gggcaattac tctttgccga
     3181 tcatcaccgt ggccggtctg tttgtcggct ttgtttttgg cttgcagggc tattacgtgc
     3241 tcaagcgcta tggttcggcc gagtcgctgg gcttgatggt gtgcctgagt ttgttgcgcg
     3301 aactcgggcc ggtggtgtcg gctttgctgt ttgccgggcg cgctggcacc tcactcaccg
     3361 cagaaattgg cctgatgaaa gccggtgagc aaatcagcgt gatggaaatg atggcggttg
     3421 acccgattca acgggtactg gcaccgcgct tctgggctgg cgtgatcacc atgccgctgc
     3481 tggcggccat gttcagcgcc actggcgtga ttggcggctg ggtggtcggg gtgctgatga
     3541 tcggtgttga tggtggtgcc ttctggagcc agattcaagg tggtgttgat gtctggcagg
     3601 acctgggcaa tggggtggtc aaaagcctgg tgtttggcgt ggcggtgacc ttcattgccc
     3661 tgctgcaagg ctataacgcg caggccacgc ccgaaggtgt cgccaaggcg accacccgca
     3721 ccgtggtgat ggcatcgctg accgtgctgg ggctggactt tatcctcacg gccatgatgt
     3781 ttacgatatg aattcaccgg gttttcaact gatgcgaaag gtgttgcatg caacgctcaa
     3841 aaaatgatgt ctgggtcggt ctgtttgtgc tgatcggggc ggtggccatt ttgtttctgg
     3901 ccctgcagtc ggccaatttg ctgagtctga gttttcagaa aagctacccg gtgctggcaa
     3961 aatttgacaa cattggcggc ctcaagccca aggcagcggt caaaagcgcc ggtgtcgtgg
     4021 ttgggcgggt tgaagacatt gtgtttgacg acaagtcgtt tcaggctttg gtgacactgg
     4081 cgctggagac ccgttacgcg tttcccaaag acagctcact gaagattttg accagtgggc
     4141 tgctgggcga ccagtacctg ggcattgaag ccggcgcaga tgataaaaat ctggccgcag
     4201 gcgacgtgat atcgagcacc cagtcggcag tggtgctgga gaatttgatc agtcagtttt
     4261 tgtacagtaa agcggccgaa gtacctgcac ctgcagagaa cggcgcaagc aaggggaaac
     4321 catgaaggat gtgatgacaa cagttgttaa aagtgcgctg ctgcgggctg gcctgatggg
     4381 tctggccttg ttgatgacgg gctgcgccag cggtccggcg gccaatcggc atgaccctct
     4441 ggagcccttc aatcggggtg ttttccagtt caacgatggc cttgaccggg ccgtcatcaa
     4501 gccggtggca accgcttacc gcgatgtttt gccgtcaccg atccgcaccg gggtgggtaa
     4561 ttttttcaat aatctgcaag atgcttggtc gtttgtgaac aacgccctgc aactcaaggc
     4621 cgagtacgct ggtaatagcc tggtgcgctt tggtgtcaac agcgtgtttg gcctggctgg
     4681 tg
//
LOCUS       DAOSLL010000376         4677 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16288_length_4677_cov_5.622674, whole genome shotgun sequence.
ACCESSION   DAOSLL010000376 DAOSLL010000000
VERSION     DAOSLL010000376.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4677
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16288_length_4677_cov_5.622674"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="PLB25_14995"
     CDS             complement(<1..390)
                     /locus_tag="PLB25_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018008464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HOE42916.1"
                     /translation="MTARQPRPGDLEPIETASRDEIAALQLQRLKWTLQHAYDNVPHH
                     KMKFDAKGVHPSDLKTLADLALFPFMTKADLRDEYPFGLLAVPREQVARLHASSGTTG
                     KSIVVGYTARDLDHWADVVARSIRAAGG"
     gene            complement(411..917)
                     /gene="paaI"
                     /locus_tag="PLB25_15000"
     CDS             complement(411..917)
                     /gene="paaI"
                     /locus_tag="PLB25_15000"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008118631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HOE42917.1"
                     /translation="MRVSPAKPHLSPQQAQDLAEATAAAMWARDQAAQALAMRIIDIR
                     PGYARLTMLVRADMVNGHHICHGGMIFTLADTAFAYGCNSYNRNTVASSCNIDFLTPA
                     REGDTLEAEAIEQSQSGRTGVYDVTVRGQHGKTIALFRGKSYRISGEVIAGLQQQDAA
                     LTGLTPTI"
     gene            complement(914..1705)
                     /gene="paaG"
                     /locus_tag="PLB25_15005"
     CDS             complement(914..1705)
                     /gene="paaG"
                     /locus_tag="PLB25_15005"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HOE42918.1"
                     /translation="MSYQTIRFEAADGIARLTLNRPDKLNSFTSAMHGELRGALDTVQ
                     GDKNIRVLVISGAGRAFCAGQDLADPDMAMGPMLPDIGNVVEQHYKPLILALQNLRVP
                     SIAMVNGIAAGAGASLALACDLVIAAKSAYFLQAFSKIGLIPDTGGTWFLPQRVGMAR
                     AIGLAMLADKLPAEKAEAWGLIWQAVDDGQLAASVDQLAKQLAAMPTKALVRTRSAMH
                     AAPNHTLEQQLSLEAGFMRELGWSADFAEGVAAFKEKRAPRFTGA"
     gene            complement(1844..2356)
                     /locus_tag="PLB25_15010"
     CDS             complement(1844..2356)
                     /locus_tag="PLB25_15010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013959115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42919.1"
                     /translation="MPKSIHLVAGMLATLTIATFFLSTLVTELFGTHETVAMVKASIV
                     MPGLFILVPAIAVTGGSGIFLSKSRQGRLIEAKKKRMPFIAANGVLIMIPCAIVLNRW
                     AAAGVFDTTFYVVQALELLVGAVNLTLMGMNIRDGLTMSGRFRVTQKKNCGLTLQFTP
                     TLRDKAAQRR"
     gene            complement(2375..2746)
                     /locus_tag="PLB25_15015"
     CDS             complement(2375..2746)
                     /locus_tag="PLB25_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HOE42920.1"
                     /translation="MLQLKPLDESVPIFQQLGADVSPVILVNIFNVAESEVPALLKAW
                     EADANWMKLQPGYISTQLHRGIAGSTVFMNYAVWESTALFSAAFNHPDFKKALEHYPS
                     SAIASPHLFSRLTVPNLCVGP"
     gene            complement(2780..4453)
                     /gene="paaN"
                     /locus_tag="PLB25_15020"
     CDS             complement(2780..4453)
                     /gene="paaN"
                     /locus_tag="PLB25_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaN"
                     /protein_id="HOE42921.1"
                     /translation="MTHALFAKHRAKLDAALNAIHTRGYWAAYSEMPSPKVYGETAAL
                     DGKKAFEAHLGQKFELNQPATSAWVATERSPYGIDLNVQYPVSDVEALITAGQAAMPS
                     WQAAGVEGRTGLCIEMLDRLNAQSFELAHAVMMTTGQGWMMAFQAGSPHAQDRGLEAV
                     TYAYREQSFVPAEATWDKPQGKNPPLVMKKHFEIVGRGVAVVVGCATFPTWNTYPGLF
                     AALATGNAVIVKPHENAVLPAAITVRTLRAVLLEHGIDPNLVSLCVPDRRESTLALVT
                     HPSVKSVDFTGGNAFGQWLIEHCRQAQVYAELAGVNNIVIDSTDAYKPMLRNLAFTLS
                     LYSGQMCTTSQALFVPASGIDTEVGHKSYDEVCADLARAIEACLAKPEVALAVLGAVQ
                     STDTLARVALADSGKLGRVLLASKKLENPEFPNAEVRTPVLLACDAADEAAYMEERFG
                     PISFVVKTADTASAVALSERVIATHGAMTAGVYSTRPEVIDAMTAATWRAKVALSINL
                     TGGVFVNQSSAFSDYHGTGGNPAANASYADSAFVANRFRVVQRRYHVGA"
BASE COUNT          898 a         1346 c         1477 g          956 t
ORIGIN      
        1 accgccagcc gcgcggatcg agcgcgccac cacgtcggcc cagtggtcaa ggtcccgggc
       61 ggtgtagccg accacaatcg acttgccggt ggtgcccgat gacgcatgca gccgggctac
      121 ctgctcgcgc ggtactgcca gcaggccaaa cgggtattca tcgcgcaagt cggccttggt
      181 catgaacggg aacagcgcca ggtcggccag ggttttcagg tcgctggggt gaaccccctt
      241 ggcatcaaac ttcatcttgt ggtgcggcac gttgtcgtaa gcgtgctgca gcgtccattt
      301 caggcgctgc aattgcagcg ccgctatctc gtcgcggctg gcggtttcga tcggctccag
      361 gtcaccgggg cggggctgtc tagcagtcat gggatttcct aatttacggt tcagatagta
      421 ggtgtcaggc ccgtcagcgc tgcatcttgc tgctgcagtc cggcaatcac ttcacccgaa
      481 atacggtacg acttgccgcg aaacaaggca atggttttgc catgctggcc gcgtaccgtc
      541 acgtcataaa cgccggtgcg tccagattgc gactgctcaa tggcttcggc ttcgagcgtg
      601 tcgccctcgc gggccggggt cagaaagtcg atattgcagc ttgaggccac ggtgttgcgg
      661 ttgtagctgt tgcagccata ggcaaaggcc gtgtcggcca gggtaaagat catgccgccg
      721 tggcagatgt ggtggccgtt gaccatgtcg gcacgcacca gcatggtcag cctggcatag
      781 ccgggccgga tgtcgatgat gcgcatggcc agcgcctgcg cggcctggtc gcgcgcccac
      841 atggcagcag cggtggcttc ggccaggtcc tgggcctgtt gtggcgacag gtgcggcttg
      901 gcggggctca ccctcatgcc ccggtaaacc tgggggcgcg cttttccttg aaggcggcaa
      961 caccttcggc aaagtcggcg ctccagccca gttcgcgcat gaaaccggct tccaggctga
     1021 gctgctgctc cagcgtgtgg ttgggtgcgg cgtgcatggc gctgcgggtc cgcaccagcg
     1081 ccttggtcgg catggcggcg agctgctttg cgagttggtc cacgctggcg gccagttgac
     1141 cgtcgtccac ggcttgccag atcagtcccc aggcctcggc cttttccgct ggcagtttgt
     1201 cggccagcat cgccagcccg atggcgcggg ccatgccgac gcgctgcggc aaaaaccagg
     1261 tgccgccggt gtcgggaatc aggccgatct tgctgaaggc ttgcaaaaag taggctgatt
     1321 tggcagcaat caccaggtcg caagccagcg ccaggctggc accggcaccg gccgcaatgc
     1381 cgttgaccat ggcaatgctt ggcacgcgca ggttttgcag cgccagaatc agcggcttgt
     1441 agtgttgttc aaccacattg ccaatgtcgg gcaacatcgg ccccattgcc atgtcggggt
     1501 cggccaggtc ctggcccgcg caaaaagcgc ggcctgcgcc gctgatcacc agcacgcgga
     1561 tgtttttgtc gccttgcacg gtgtcaagtg cgccgcgcag ttcaccgtgc atggcactgg
     1621 taaagctgtt gagcttgtcg gggcggttga gcgtgaggcg ggcgatgccg tctgcggctt
     1681 cgaagcggat agtctggtag ctcatgagga tgccttaaag agaatgacga tgccgaactg
     1741 ttcgaatgac cttttgtcgc gtcgattgca gcctgcccga cagaatcatc ggccagacgg
     1801 caggcaactg gtggtctatt gcgccttttc ctgaacgtag aagttacctg cgctgcgcgg
     1861 ctttatcgcg cagcgtcggt gtgaattgca gggttagacc gcaatttttt ttttgggtga
     1921 cacgaaacct cccgctcatt gtcaagccgt cacggatgtt catacccata agtgtgaggt
     1981 tcaccgcgcc aaccaaaagc tccaatgctt gtacaacgta gaaagtggtg tcgaagaccc
     2041 cggcagccgc ccaacggttg aggacaatgg cgcatggaat catgatcaga actccatttg
     2101 ctgcaatgaa aggcatgcgc ttcttcttgg cctcgatcag gcgcccttga cgtgacttgg
     2161 acaggaatat gccgctgccc ccagtcaccg ctatagcagg caccagaatg aacaaaccgg
     2221 gcatgacaat cgaagctttc accattgcga cggtctcgtg cgttccaaaa agctccgtaa
     2281 ccagcgtgga aaggaagaac gtggcaatgg tcagggttgc gagcatgcca gcgacaagat
     2341 gtatgctctt tggcattgca tttcctcctg acatctatgg gccgacacaa agatttggca
     2401 cagtcagccg actgaacaga tgcggcgagg cgatggcgga tgaggggtag tgctccaggg
     2461 cctttttgaa gtctggatgg ttaaaagcgg cgctaaagag cgcagtcgat tcccaaacag
     2521 cgtagttcat gaatacagtg ctgcccgcga tgcctcgatg taactgcgtt gagatatacc
     2581 ccggctgcaa tttcatccaa ttggcatccg cctcccacgc cttgagcagt gctggaactt
     2641 ctgactctgc aacattgaat atgttgacaa ggataaccgg cgacacgtcg gctccgagct
     2701 gttgaaaaat tgggacgctt tcatcgagcg gcttaagttg aagcattgac agtctcctta
     2761 ttgtgtggtt gtgagttgct tacgctccaa catgatagcg ccgttgcact acccgaaaac
     2821 gattcgccac aaaggccgag tcagcgtaag acgcgttggc cgccgggtta ccgccggtgc
     2881 cgtggtagtc agaaaacgcg ctggactggt tgacaaacac gccgcctgtc aggttgatcg
     2941 acagcgccac cttggcgcgc caagtggctg ccgtcatcgc atcgatcact tcagggcggg
     3001 tcgaatagac accggcggtc atcgcaccat gggttgcgat gacccgctct gacagggcga
     3061 ctgcgctggc ggtgtcagcg gtctttacga caaaactgat cgggccaaag cgctcttcca
     3121 tgtaggctgc ctcgtcggcg gcatcgcagg ccagcagcac cggggtgcgc acttcggcat
     3181 tgggaaactc ggggttctca agctttttgg aggccagcaa gacacggcca agcttgccac
     3241 tgtcagccaa tgcgacgcgg gccagggtgt cagtagattg caccgcaccg agcaccgcca
     3301 gcgctacttc gggcttggcc aggcaagcct caatggcccg ggccaggtcg gcgcagacct
     3361 cgtcgtaact cttgtggccg acttcggtgt cgatgccgct ggcgggcaca aacagcgcct
     3421 gcgaggtggt gcacatctgg ccggaataca gcgacagggt gaacgccaga ttgcgcaaca
     3481 ttggcttata ggcatcggtc gagtcgatca cgatgttgtt gaccccggcc agctcggcat
     3541 acacctgggc ctgccggcag tgctcgatca gccactggcc aaaggcattg ccgccggtga
     3601 agtccaccga tttgacgctg gggtgcgtca ccagcgccag cgtggattcg cgcctgtcgg
     3661 gcacacacag gctgaccagg ttggggtcaa tgccgtgctc cagcaacacg gcacgcagcg
     3721 tgcgcaccgt gatggcagcg ggcaacacgg cgttctcgtg cggcttgacg atcaccgcat
     3781 tgccggtggc cagcgcggca aacagaccag ggtaggtgtt ccaggtcgga aaagtggcgc
     3841 agccgaccac cacggcaaca ccccggccaa caatttcgaa gtgctttttc atcaccagcg
     3901 gcgggttctt gccctgcggc ttgtcccagg tggcttcagc ggggacaaaa ctttgctcgc
     3961 ggtaggcgta ggtcacggct tccagaccgc ggtcttgcgc gtgcgggctg ccagcctgaa
     4021 atgccatcat ccagccctgg ccagtggtca tcatgacggc gtgggccagc tcgaagctct
     4081 gtgcgttcag ccggtccagc atctcaatgc acaggccggt gcggccttca acgccagccg
     4141 cctgccaact gggcatggcg gcctggccgg cggtgatcag cgcttcgacg tcggacaccg
     4201 ggtactgcac gttcaggtcg atgccgtaag gggatctttc ggttgccacc caggcgctgg
     4261 tggcgggttg gttgagttca aatttttgac ccagatgggc ttcaaaggct tttttgccgt
     4321 cgagcgcagc ggtttctcca tagaccttgg ggctgggcat ttcgctgtag gcggcccagt
     4381 agccccgggt gtggatggcg ttgagggcgg catcgagctt ggcacggtgt ttggcaaaga
     4441 gggcgtgggt catgatggtt tcctgaaaaa gttcgggtcg atatcaaaaa gatagctggt
     4501 gacgcttatg tgacatgggc tagaggccaa aaaggcataa atcgcgaagc ggtgaaccga
     4561 ttgtcatccc ggcgacggct ggcatctgct gcctgctgcg cactgaggcg ctgcgagcaa
     4621 cacaggctct atgccgtttt gtgtggaact tgacatcaca agcagcgatg gactagc
//
LOCUS       DAOSLL010000377         4662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16346_length_4662_cov_4.832212, whole genome shotgun sequence.
ACCESSION   DAOSLL010000377 DAOSLL010000000
VERSION     DAOSLL010000377.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16346_length_4662_cov_4.832212"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1267
                     /locus_tag="PLB25_15025"
     CDS             <1..1267
                     /locus_tag="PLB25_15025"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HOE42922.1"
                     /translation="LLHLPNRARFIEEIANCVQADQRRYALAVVDIDDFASVNDLMGH
                     AYGDELLRAYACQLSKQLGSELVLARVGSNTFGLLGTSDTLLPERIAATVRDAVEVFG
                     QPHRLSVTSGIALLDASDCSGADWLKNAGIALKNAKRHSRGGHVFYSASMAALSRSRA
                     LLLANLHTAFGQSHLFLAYQPQVDLQSGALIGMEALLRWRKEDGSMVPPDQFIPIAEQ
                     SGLIVSLGDWVLQTACRDMRSLRDQGLATCRMAVNVSAVQFQSPGYFERVLAALDQSA
                     LRADELELEITESVAMLGSDAVAIMLGRLRESGIAVAIDDFGTGYSSLSCLEKLPLDR
                     IKIDRAFVIQMSHQGGPRIAEMITQLGHKLGLRVLAEGIEDALAWQALRDMGCHEGQG
                     FFISRPLDYAALCTWLAQRPRSAFEFGVV"
     gene            1280..2404
                     /locus_tag="PLB25_15030"
     CDS             1280..2404
                     /locus_tag="PLB25_15030"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE42923.1"
                     /translation="MRHICWGGLQVVAALALTLALAGCDKAPVSLGFLGGLTGSAADL
                     GTAGRDAALLAVEQINQQGGLLGASVKLHEFDDQQQPGTLPVLLEAVKKAGVLALVGP
                     MTSSVAKAWIPLADQARLLTISPTVTSSDFTGHDDYFFRVISDTRSYARASAAHYAEH
                     QHWSRYAVVFDETNAAYTRSWLQHFEQAMKERGVALVSASGFGLPQSLPPALLVQQVL
                     MQHPDALVLIANATDAAQMVQLVRKKDQQLPIIAAEWSATAQLLLLGGAAVEGLRISQ
                     FMDHQSQQPEYLKFVADFEKRFGRAPGFAEVAAYDAMRVLLEALVQQRPGESIKASLL
                     RIRQFKGLQQSVVFDDYGDSSRQVVMTEVKGGRYQVIGRP"
     gene            2401..>4662
                     /locus_tag="PLB25_15035"
     CDS             2401..>4662
                     /locus_tag="PLB25_15035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE42924.1"
                     /translation="MIQDAVGRARSGQAGITLPASYSLRTMLMVQFLAVAVLAGIVAT
                     LLMVVWRLPLAQKQVQTEQARVASMVLQQLEVLLDTTETLTASIGQMLADERSAASRA
                     TAAALIARVSGSAELFENLYVLNQQAKVIAMGSSARPGLQMADWIGNDFSGLPVVQAV
                     RRSRGLTWSEQFQSPVLGIPVVAVALPAGEDIVFAEVSVRRLVDFVHSSNSLDGLMVL
                     VVDGKGETVAAPDMQLARSRSNLSNIAVVRAALDGAPIFGTFSLAGQSYSGTARRSNR
                     LGWVVVSAYPESVADAPRRVAVVITSVTLALSVGFGMFLFGAFANLMQRRVQRTVAYA
                     QAVAQGQYEPPGDKSVVREMQQLDASLEQMARTIQRREQQLRAIVDTTPTLAIQWYDR
                     SGRVLDWNPASVTMFGWSREEALGQTLDQLIYSREQQQWFQDVLAGIEKTGQPFGPFE
                     ATTHNRRGEERQILATTFSIPDISHGLQFVCMDIDITDMKQKEQEIRANENKFNVFFN
                     ASPVAVAVLEKRDKAYIHTDVNQAWEQLIGYSHQQALAMHLADSGFLVDSLVGSELLA
                     QVESQQIVTRVETWARRANGAQFLAEGSLGRVCIAGRELVIYSLHDISDKRQMENDLR
                     ELNAELEERIRKRTESLSQANLDLQKAFTDLRLMQDHLVQSEKLASLGAMVAGIAHEL
                     NTPIGNGLMAISTLEQRALTFRVQVAQGLRRSDLEAFLGQVETAGMIATRNLARAAEL
                     IAGFKRVAVDQTSA"
BASE COUNT          867 a         1321 c         1493 g          981 t
ORIGIN      
        1 gttgctgcat ttgcccaatc gggctcgctt cattgaggaa attgccaact gcgtccaggc
       61 cgaccagcga cgctatgctt tggcggtggt cgatatcgat gattttgcgt cggtcaacga
      121 tttgatgggt cacgcttacg gtgacgaact gctgcgggct tatgcctgcc aactgagcaa
      181 acaactgggc tcggaactgg tgttggcgcg ggttggcagc aacaccttcg ggctgctggg
      241 caccagcgac acactgttgc cagagcggat tgccgccacc gtgcgggatg cagtggaggt
      301 ttttggccag ccacatcgcc tttcggtgac gtccggcatt gccctgcttg acgcttctga
      361 ctgtagtggc gcggactggc taaaaaatgc cggcattgcg ctgaaaaatg ccaagcgtca
      421 tagtcgtggt gggcatgtgt tttattctgc cagcatggcc gccctgtcgc gcagccgggc
      481 gttgttgctg gcaaacctgc atacggcatt tggtcagagt catttgttcc tggcttacca
      541 gccgcaggtt gacctgcagt ccggtgcgct gattggcatg gaagccctgc tgcgttggcg
      601 caaggaagac ggcagcatgg tgccgccaga tcagttcatc ccgattgccg agcagtccgg
      661 cctgatcgtc agcctgggcg actgggtatt gcaaaccgct tgtcgcgaca tgcgcagcct
      721 gcgggaccag ggcctggcca cttgccgcat ggccgtcaac gtctcggcgg tacagttcca
      781 gagcccgggc tactttgagc gcgtgctggc cgcgcttgac cagtcagccc tgcgtgccga
      841 cgaactggag ctcgaaatca ccgagtccgt tgccatgctc gggtcggatg ccgtggccat
      901 catgctgggg cgcttgcgag aaagcggcat tgccgtcgcc attgatgact ttggcaccgg
      961 ttactcttcc ctgagttgtc ttgaaaagct gccgcttgac cggatcaaga ttgaccgtgc
     1021 atttgtcatc cagatgagtc atcaaggcgg gccccggatt gccgaaatga tcacccaatt
     1081 gggacacaag ctcgggcttc gggtgttggc tgaaggcatc gaggatgcgc tggcctggca
     1141 ggccctgcgc gacatgggtt gccatgaagg ccagggcttt ttcatctcgc ggccactcga
     1201 ttacgcagcc ctttgcacct ggttggcaca gcgcccgcgc agcgcctttg aattcggcgt
     1261 ggtctgagtg agcaacacca tgcgacacat ttgttggggt ggtttacaag ttgtggctgc
     1321 gctggccctg accttggcgt tggctggctg cgacaaggca ccggttagcc tgggctttct
     1381 gggcggtttg actgggtcgg ccgctgacct gggtactgcc ggtcgcgacg ctgccttgct
     1441 ggcggtcgaa caaatcaatc aacaaggtgg cttgctgggt gccagcgtca agctgcatga
     1501 gtttgatgat cagcagcagc ccggcacact gccggtactg ctggaagcgg tcaaaaaagc
     1561 cggtgttttg gcgctggtcg gcccgatgac cagcagtgtt gccaaagcct ggataccgct
     1621 ggccgatcag gccaggttgc tgaccatttc gcccaccgtc accagcagtg acttcaccgg
     1681 tcacgacgac tacttctttc gggtcatctc ggacacccgc agttatgcgc gtgccagtgc
     1741 cgcccattac gccgagcacc agcactggag ccggtatgcc gtggtctttg acgaaaccaa
     1801 tgcggcctac acccgcagtt ggctgcagca ttttgagcag gccatgaagg agcgtggcgt
     1861 ggcgttggtg tctgccagcg ggtttggttt gccgcaaagt ctgccaccgg cattgctggt
     1921 gcagcaagtc ttgatgcagc acccggatgc cctggtgctg atcgccaacg ccaccgatgc
     1981 ggcccaaatg gtgcagttgg tgcgcaagaa agaccagcag ttgcccatca tcgccgccga
     2041 atggtcggcc acggcacaac tgttgctgct gggcggtgcc gcagttgaag ggctgcggat
     2101 ttcccagttc atggaccacc agagccagca gccagagtac ctgaagtttg tcgctgactt
     2161 tgaaaagcgc tttggccgcg cacctggctt tgccgaggtg gctgcatatg acgcgatgcg
     2221 cgtcttgctt gaggcgctgg tgcaacagcg ccctggcgag tcgatcaaag ccagcctgct
     2281 caggattcgc cagttcaagg gtttgcagca gtcagtggtg tttgatgact acggagattc
     2341 gagtcgacag gtcgtgatga ccgaggtcaa agggggtcgt taccaggtta ttggcaggcc
     2401 atgatccaag acgctgttgg ccgagcccga agtgggcaag ccgggataac gctgcccgcc
     2461 agctattcgc tgcgcaccat gctgatggtt cagtttctgg cggtcgcggt gctggcaggc
     2521 attgtggcca ccttgttgat ggtggtctgg cgtttgccgc tggcccaaaa gcaagtgcaa
     2581 accgaacagg ccagggtggc cagcatggtg ttgcaacagc ttgaagtcct gctcgacacc
     2641 accgagacgc tgaccgcttc gattggtcag atgctcgccg acgagagatc tgccgcctcg
     2701 cgcgcaacgg ctgcggcgct gatcgcccgc gtgtctggtt cggccgaact gtttgaaaac
     2761 ctttatgtgc tcaaccagca agcgaaagtg atcgccatgg gctcgtcagc ccgccccggc
     2821 ctgcaaatgg cggactggat tggcaacgac ttcagtggct tgccagtggt gcaagcggtg
     2881 cgccgcagcc gtggcctgac ctggagcgag cagtttcagt ccccggtgtt gggcatcccg
     2941 gtggttgccg tggcactgcc tgctggcgaa gatatcgttt ttgctgaagt atcggtgcgt
     3001 cggctggttg actttgtcca cagttccaac agtctggatg gcttgatggt gctggtggtt
     3061 gacggcaagg gcgagacggt ggcggccccc gacatgcagt tggcgcgtag ccgcagcaac
     3121 ctgtcgaata ttgccgtggt gcgcgcagcg ctggacggtg cccccatttt tggcaccttc
     3181 agcctggctg gccaaagcta ttccggcacg gccaggcgct ccaatcggct gggttgggtg
     3241 gtggtgtcgg cttaccctga atcggtggcg gatgcgccac ggcgcgtggc ggtggtcatc
     3301 acctcggtca ccctggcgct gtcggtcgga tttggcatgt ttctgtttgg tgcttttgcc
     3361 aacctgatgc agcgccgcgt ccagcgcacc gtggcctatg cccaggcggt ggcacagggg
     3421 caatacgagc cgcccggcga caaatcagta gtgcgcgaaa tgcagcaact cgatgccagt
     3481 ctggagcaaa tggcgcgaac catccagcgc cgcgagcagc aactgcgcgc catcgttgac
     3541 accaccccga cgctggcgat ccagtggtat gaccgcagcg ggcgggtgct tgactggaat
     3601 ccggcatcgg tgacgatgtt cggctggagc cgtgaagagg cactgggcca gacgcttgat
     3661 caactgatct acagccgaga gcaacagcag tggttccagg atgtgctggc cggtatcgaa
     3721 aaaaccggac agccttttgg tcccttcgag gccacgacgc acaaccgccg tggtgaagaa
     3781 cggcagattc tggccaccac attttccatt cctgacatca gccatgggct tcagtttgtc
     3841 tgcatggaca tcgacatcac cgacatgaag caaaaagaac aggaaatccg cgccaacgag
     3901 aacaagttca atgtcttttt caacgccagt ccggtggcag tggcggtgct cgaaaagcgt
     3961 gacaaggcct atattcacac cgatgtcaac caggcttggg agcagttgat tggctactcg
     4021 caccagcagg cactggcaat gcacctggca gactcaggct ttctggtgga ctcgctggtc
     4081 ggctcggagt tgctggcgca ggtcgaaagc cagcagatcg tcacccgggt ggaaacttgg
     4141 gcgcgtcgcg ccaatggcgc acagtttttg gccgaaggat cactcgggcg ggtctgcatt
     4201 gccgggcgcg aactggtgat ttattctttg cacgacatca gcgacaagcg gcagatggaa
     4261 aacgacttgc gcgagctcaa tgccgaactt gaagagcgta tccgcaaacg aaccgaaagc
     4321 ctgagccagg ccaatctgga cttgcagaag gccttcaccg atttgcggct gatgcaagac
     4381 cacctggtgc aatctgaaaa gcttgcctcg ctcggtgcaa tggtggcagg cattgcgcac
     4441 gagctcaaca cgccgattgg caatgggctg atggcgattt ccacgctgga gcagcgcgcc
     4501 ctgacgttcc gggtgcaggt ggcgcagggg ctgcgccggt ctgatctgga ggccttcctg
     4561 ggtcaggtcg agacggccgg catgattgcc acgcgtaacc tggcccgggc cgccgaattg
     4621 attgccggtt tcaagcgggt tgcggttgat cagaccagcg cg
//
LOCUS       DAOSLL010000378         4662 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16350_length_4662_cov_3.418277, whole genome shotgun sequence.
ACCESSION   DAOSLL010000378 DAOSLL010000000
VERSION     DAOSLL010000378.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16350_length_4662_cov_3.418277"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="PLB25_15040"
     CDS             <1..214
                     /locus_tag="PLB25_15040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42925.1"
                     /translation="LLLIDLEAKDTLAGAAAYSRSVRIQGIGRGGKERAETLEIRSLN
                     NARAARARKGKLADLGFKPVAITRIE"
     gene            complement(281..616)
                     /locus_tag="PLB25_15045"
     CDS             complement(281..616)
                     /locus_tag="PLB25_15045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOE42926.1"
                     /translation="MATACGTGIFRLARAFFLPRYKQMFGYAEHEFENHPEAWSSRIH
                     PDDAPGVMTALWPCLDGKDGTASVEFHMPCKDGSWLWTLGRGRVIARDASGKPTRMIG
                     TNADITERK"
     gene            complement(977..1633)
                     /gene="adk"
                     /locus_tag="PLB25_15050"
     CDS             complement(977..1633)
                     /gene="adk"
                     /locus_tag="PLB25_15050"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HOE42927.1"
                     /translation="MKLILLGAPGAGKGTQATFICQKYGIPQISTGDMLRAAVKAGTP
                     LGVQAKQVMDAGGLVSDDLIINLVKERIAQGDCANGFLFDGFPRTIAQADAMKAAGVK
                     LDYVLEIDVPFSAIVERMSGRRSHPPSGRTYHVKFNPPKVAGVDDITGEPLVQRDDDK
                     EETVMKRLDVYSAQTRPLVAYYSDWAKSDPAAAPKYRAISGSGGVDEITASAFAALAS
                     "
     gene            complement(1894..2658)
                     /gene="kdsB"
                     /locus_tag="PLB25_15055"
     CDS             complement(1894..2658)
                     /gene="kdsB"
                     /locus_tag="PLB25_15055"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HOE42928.1"
                     /translation="MSFTVLIPARLDSARLPNKPLADIGGIPMVVRVAQRVLSLAAIR
                     ASRKAPRVVVAADGQQIMSACQAHGIEAVLTRTDHPSGSDRLAEACTLLGLPDESIVV
                     NVQGDEPLMDPALVAAVANLLRKTPIARMSTAAHVIESVADFHNPNVVKVVIDAHNMG
                     LYFSRAPISYPRDTPELLPQPAPLRHIGIYGYRVGFLKLFPTLSRAPMEVTESLEQLR
                     ALWHGYRIAVHVTAHAPGPGVDTPQDLERVRNLFGA"
     gene            complement(2655..2837)
                     /locus_tag="PLB25_15060"
     CDS             complement(2655..2837)
                     /locus_tag="PLB25_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HOE42929.1"
                     /translation="MDTRLLELLVCPVTKGPLEYRRETQELTSRSARLAYPVRDGIPI
                     LLENEARPMTDEELGL"
     gene            complement(2818..3900)
                     /gene="lpxK"
                     /locus_tag="PLB25_15065"
     CDS             complement(2818..3900)
                     /gene="lpxK"
                     /locus_tag="PLB25_15065"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HOE42930.1"
                     /translation="MLDAPGNPGWQQTLTQAWTRRGLLAWLLWPVSLLYRLLVSARLA
                     LYRAGWLACARLPVPVIVVGNVVAGGAGKTPVVLAIAAHLQQQGIRVGIISRGYGRST
                     QACLEVTPDRPAQEVGDEPALMQQRTGAPVFVASRRVQAAQALLTGYPQTQVILSDDG
                     LQHLRLGRDLEIGVFDDRGVGNGFLLPAGPLREPWPRHLDLLLHTGAHPAFAGFQGQR
                     NLARQALRADGSTLALADLAAATRLGAGAGIKPLMALAAIAQPEAFFAMLRAQGLTLA
                     KTLALPDHYDFGSWSCNEYKGYSLICTEKDAVKLWPLEAQALAVPLQFVVPQAFWQVF
                     DARLEALLSAHTPTKTLSLAHGHKTA"
     gene            complement(3956..4384)
                     /locus_tag="PLB25_15070"
     CDS             complement(3956..4384)
                     /locus_tag="PLB25_15070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOE42931.1"
                     /translation="MNFRPHPKDEPEINLIPFIDVLLVILIFLMLSTTYSKFTELQLR
                     LPVADVDAQRDYPKEVLVAVSSDGNYSVNKVAVGGRSVAELSQALQDGAKAGKESVVI
                     IHADAAARHQAVITVMEAARRVGLTQITFASQSSAAGATP"
     gene            complement(4397..>4662)
                     /locus_tag="PLB25_15075"
     CDS             complement(4397..>4662)
                     /locus_tag="PLB25_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOE42932.1"
                     /translation="PLMGLFGTVVGMIEIFGSQTPTGATGGNPAQLAHGISVALYNTA
                     FGLVVAIPSLIFWRYFRARVDEYLLTLEIAAERLARHLNTLRM"
BASE COUNT          937 a         1424 c         1414 g          887 t
ORIGIN      
        1 cctgctgttg attgacctgg aagctaaaga cacgttggca ggtgctgctg cctacagccg
       61 cagtgtgcgc attcagggta ttggccgggg cggcaaggag cgcgccgaaa cgctggaaat
      121 ccgtagcctc aacaatgccc gtgccgcccg cgcccgcaaa ggcaaactcg ccgatcttgg
      181 cttcaagcca gtggcgatca cgcggattga gtagtccggc aaagttgttt tcatcgcatc
      241 tgcgacattc ccgcttgctt tcacgaatga tcttttcggc ttatttgcgc tcggtgatgt
      301 cggcattggt gccaatcatg cgcgtaggtt tgccgctggc atcgcgggca atcaccctgc
      361 cgcgccccag cgtccataac caactgccat ccttgcacgg catgtgaaat tccacgctgg
      421 cagtaccatc tttgccatcc agacagggcc acaaggccgt catcacgccg ggtgcatcat
      481 ccgggtgaat gcggctggac catgcctcgg gatggttctc gaattcatgc tcggcatagc
      541 caaacatttg cttgtagcgg ggtaaaaaaa aagccctcgc cagtctgaag attccagtcc
      601 cacaggccgt cgccaccact ttcgagggca aattgccagc gttcttcgct ttgtttctgt
      661 gcagcttcag gcagcctgac cgggcggctc agtgcgggtt ggggaaccgc cgacgcgacg
      721 gctgcgcggt gaaactgccc aaggcggcga cacgaacgtg cgcactcgct tactgagcga
      781 aacttgcttt tgattcaccg tgggcctgtc attgaaggcc cttgcaagat catcacaggc
      841 caactcgtca gcgcccagat gggtcgtgtg ccgataccca ggcagccagc gcaccaaccg
      901 caatcgcccc gaaaaagccc ggcattgcag cgggcatcag gcttcgccaa gcaaccgatc
      961 tgcgctgctt ccgggttcaa ctggccaatg ccgcaaaggc acttgccgtg atttcatcga
     1021 ctccaccgga gccgctgatg gcccggtact tgggggcagc ggcagggtct gacttggccc
     1081 agtcggaata ataggccacc agcggacggg tctgggcgct gtacacatcg agccgtttca
     1141 tgacggtttc ttccttgtcg tcgtcacgct gtaccagtgg ctcgccggtg atatcgtcaa
     1201 cgccagccac cttgggcggg ttgaacttga catggtaggt acggcccgaa ggtgggtgcg
     1261 accgcctgcc actcatgcgc tccacgatgg cactgaaggg cacgtcaatc tccagcacgt
     1321 agtcgagttt gacaccggca gccttcatgg catctgcctg ggcaatggtg cgtggaaagc
     1381 cgtcaaacag aaagccgttg gcgcagtcgc cctgggcaat acgctccttg accaggttga
     1441 tgatcaggtc atcgctcacc aagccaccag catccatcac ctgtttggcc tgaacgccca
     1501 acggcgtgcc tgccttgaca gcagcgcgca gcatgtcacc ggtggaaatc tgcggaatgc
     1561 cgtatttttg gcagatgaat gtggcttggg tgcccttgcc tgcgccgggt gcgcccaaca
     1621 aaatcagttt catgcgtgtc ctcgggtgaa tattgaaaat cgcttctaag gtgggcatcc
     1681 ggtggggtag cccgggtctg acagattcag gacatggtca tccatgactt ctccatttgg
     1741 ctcgtcgggt ttgggcacac cagcgctttg cgcctggcat tgcatcccga ttccagcggc
     1801 caaatgggta acctattttt gaccctaacc tgagacagac ttggaaagcg agaatagcac
     1861 gcgcgagctt tgcgctgact tacaggggct gcgtcaagca ccaaacaggt tgcgcacccg
     1921 ctccaggtct tgcggcgtgt caacgccggg tccgggtgca tgtgccgtca catgaacggc
     1981 aatgcggtag ccgtgccaca aggcacgcaa ctgttccagt gactcggtga cttccatggg
     2041 agcgcggctc agggtcggaa acagcttcaa gaaaccaacc cgatacccat aaatgccaat
     2101 gtgtcgcagt ggcgctggct gcggcagcag ctcgggtgtg tcacgcgggt aggaaattgg
     2161 tgcgcgactg aaatacaggc ccatgttgtg cgcgtcaatc accaccttga ccacgttggg
     2221 gttgtgaaaa tcagcaaccg attcaatcac gtgggcggca gtgctcatgc gcgcaatagg
     2281 ggtctttcgc aacaagttgg cgaccgccgc gaccagcgcc gggtccatca gcggttcatc
     2341 accttgcaca ttcaccacaa tcgattcgtc cggcaagccc agcagcgtgc aggcctcggc
     2401 cagccggtca ctgccagacg ggtgatcggt gcgggtcagc acggcctcaa tgccatgcgc
     2461 ctggcaggca gacatgatct gctggccgtc ggcagccacc accacacgcg gggctttcct
     2521 ggatgccctg attgcagcaa gagacaacac acgctgagcc acccgcacca ccatgggaat
     2581 gccgccgatg tcggccagcg gtttgttggg caggcgggcc gagtccagcc gcgccgggat
     2641 cagcacggta aagctcatag cccgagttct tcgtcagtca tgggtctggc ctcgttttcc
     2701 agcaggattg gaatgccgtc gcgcaccggg taggccaggc gggcactgcg cgaggtgagt
     2761 tcttgcgtct cgcgccggta ttccagcggt cctttggtga ccgggcaaac caacaattca
     2821 agcagtcttg tgtccatggg ccaatgatag cgttttggtt ggtgtgtggg cactgagcaa
     2881 cgcttcaagt cgtgcatcga aaacctgcca aaaggcctgc ggcacaacaa actgcagcgg
     2941 caccgccagc gcctgcgctt ccaggggcca cagcttgacc gcatcttttt cagtacaaat
     3001 aaggctgtag cccttgtact cattgcatga ccagctacca aagtcatagt gatccggcag
     3061 cgcaagggtt tttgccaggg tcaggccctg cgcgcgcaac atggcaaaaa aagcctctgg
     3121 ctgggcaatt gcggccagcg ccatcaacgg tttgatgcca gcaccagcgc ccagtcgggt
     3181 agcggctgcc aggtcggcca gtgccagcgt cgacccgtct gcgcgcaatg cctgccgggc
     3241 cagattgcgt tgcccctgaa aaccggcaaa agccggatgt gcacccgtgt gcagcagcag
     3301 gtcaaggtgg cgcggccagg gctcgcgcaa ggggccggcc ggcagcaaaa aaccattgcc
     3361 gacaccgcgg tcgtcaaaca cgccgatttc caggtcacgt cccaggcgca gatgctgcag
     3421 tccatcgtcg ctgaggatga cctgggtttg tgggtagcca gtcagcagtg cctgcgccgc
     3481 ctgcacccgc cgggatgcga cgaaaactgg cgcgccggtg cgctgctgca tcagcgctgg
     3541 ctcgtcgccg acctcttggg cagggcgatc aggcgtgact tccaggcaag cttgcgtgct
     3601 gcgcccgtag cctcttgaga tgatgccgac ccgaatgcct tgctgctgca aatgcgctgc
     3661 aatggccagc accactggtg ttttgccagc gcccccggcc accacattgc cgaccacgat
     3721 gacgggcacc ggcagccggg cacaagccag ccagccagca cggtagagtg ccaggcgagc
     3781 gctcaccagc aagcggtaca acagcgaaac cggccacagc agccaggcta gcaggccgcg
     3841 cctggtccag gcctgggtta acgtctgctg ccaaccgggg ttgcctggcg catcaagcat
     3901 ggtttcaatg cactgtcaac aagccagcag gacgatcagg ccagccagcc ggggatcagg
     3961 gcgtggcacc agccgcagat gactggctgg caaaggtgat ttgggtcagg ccaacgcgcc
     4021 gggcggcttc catcaccgtg atcactgcct gatgccgggc ggcggcatcg gcgtggatga
     4081 tgaccaccga ttccttgccc gccttggcac cgtcttgcaa tgcctgactg agctcggcca
     4141 cgctgcgccc gccaaccgcc actttgttga cgctgtaatt gccgtcgctg ctgacggcca
     4201 ccagcacttc tttggggtag tcgcgctgcg catcaacgtc ggctaccggc aggcggagct
     4261 gcaactcggt aaatttgctg taggtggtgg agagcatcaa aaagatcaag atcaccagca
     4321 gcacgtcaat gaacgggatc aggttgatct cgggttcatc cttggggtgt ggacggaaat
     4381 tcatgcggac cggtggctac atgcgcaggg tattgagatg ccgggccagg cgctcggcgg
     4441 caatttccag cgtcagcagg tactcatcga cgcgggccct gaaataacgc cagaagatca
     4501 gcgacggaat ggccaccacc aggccaaagg cggtgttgta caaggccacc gagatgccat
     4561 gcgcgagttg cgccgggttg ccgccggtgg ccccggtcgg ggtttgcgag ccaaaaatct
     4621 cgatcatgcc aacgaccgtg ccaaacagcc ccatcagcgg tg
//
LOCUS       DAOSLL010000379         4656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16372_length_4656_cov_5.130624, whole genome shotgun sequence.
ACCESSION   DAOSLL010000379 DAOSLL010000000
VERSION     DAOSLL010000379.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16372_length_4656_cov_5.130624"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..542)
                     /locus_tag="PLB25_15080"
     CDS             complement(36..542)
                     /locus_tag="PLB25_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42933.1"
                     /translation="MTQNLFATHWLVNTLLDEGFVAHKITELTKLPIPYGLSDWLDVN
                     FDAPSLSLRSEHQLENSFIGPLLAQLGWTAVPQQNIVVQGKLAKPDWCLLLAAEQGNT
                     LIASKDHTLITAICESKAWGKTLDTGKADRTYNPHHQLQDYLSTLRVRFGFLTNGRIW
                     RIYPIFLS"
     gene            561..1190
                     /locus_tag="PLB25_15085"
     CDS             561..1190
                     /locus_tag="PLB25_15085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42934.1"
                     /translation="MRHADALLAQGCQRHGGDVVRQREVLRATVYRPSHTSDLQRTSA
                     AKPDEPNAEVIDDHVLYGESNVMVRFRVLDWLWKPVGRIVRLLIVHHPVRGTIFLLSI
                     DTSLEPLETLQLYSYGFKIELGFRQAVHVIGTYADHFWMLGMKPPCRGSGDQYLQRTT
                     DAYRGPSAQSCLPTMCMFSSAVSHNGCCSAWPSITQPKFGAAFAVGFVP"
     gene            1714..2010
                     /locus_tag="PLB25_15090"
     CDS             1714..2010
                     /locus_tag="PLB25_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE42935.1"
                     /translation="MNGTTVLVVDDAASVRATVSIALKGGGYEVIEASDGNDALRKPQ
                     IKRVHLIISDVNMPGMDGITLPKRLREQAATKQGLVSEMAIPTGMLSSLNAMEF"
     gene            2012..4549
                     /locus_tag="PLB25_15095"
     CDS             2012..4549
                     /locus_tag="PLB25_15095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE42936.1"
                     /translation="MNFKDMKIATRLGFTFGLVLVVTIIAAVLTLSKVADIQENLRDI
                     VQDNNVKVRLSNEMAASTHIVARVIRTILLVKEKELREQRRKSIADARDNYNKLWAEL
                     EKMPVSEAGKAIRKKVSDARSIVLELNNRVIEFTMADKDSEATELLLKEASPAIQKML
                     DALNENIAFQQAETQKVYAEAEAQYQSTRSLLIAANALVVLLAALSGWLVTRSITRPL
                     HLAVQAADDVAAGKLDGKLPMSGNDETGQLLQSFSKMQGVLGQFQAAQAEMARQHQAG
                     MIDFAMPAQELPGDYAAMARSTNELVQAHIALNAKVIDLALSYSEGKLDTSMERLPGQ
                     KARITEAMNKVQAIMQQAASAATFNLRIRNALDSLAANVTVSNADGLLVHATPPAKAL
                     LKLIAGPGFDADAFYGNKLSSLFTHGQSAAQFDRAFKTGETEDMDVAGRKLRLQARPV
                     LDASGQPIGRVTQWFDRTDEIAAEEEVAAIVAAAGAGDFSQRLSTTGKSGFLEMISVG
                     MNELIATSEQGLGDVAEVMAAFADGDLTKRIERDYQGLFGKVKDSVNTTAENLTRVIG
                     EVHAASDALLGAANQVSATAQSLSQAASQQAASVEETSSQIEVMSTSINQNSDNAKVT
                     DSVATKTSQEAVEGGAAVSSTVDAMKQIALKIGIVDDIAYQTNLLALNAAIEAARAGE
                     HGKGFAVVAAEVRKLAERSQEAAKEIGGLASNSVTTAERAGKLLDEIVPSIQKTSELV
                     QEIASESMAQICSVDQIGSAMGQLSKATQQNASASEELAATSEELTGQAKQLQDSIAF
                     FNVADGSNRECARLESTPKRRMTPPRLLSPLTPTAVRSDRGNNFRPY"
BASE COUNT         1113 a         1239 c         1332 g          972 t
ORIGIN      
        1 ttatcatgct tgagttttta agactttttt gggcttcaac tgaggaaaat aggataaatg
       61 cgccagatgc gcccgttggt caaaaaaccg aagcgcaccc gcagggtgct gaggtagtcc
      121 tgcaactggt gatgcgggtt gtaggtgcgg tcggctttgc cggtgtcgag tgttttaccc
      181 caggctttgg attcgcaaat ggcggtgatc agggtgtggt ccttgctggc aatgagggtg
      241 ttgccctgtt cggccgccag cagcaggcac cagtctggct tggccagctt gccctgaaca
      301 acgatgtttt gctgtggaac agcggtccag ccgagttggg cgagcagggg accaataaag
      361 ctgttttcaa gctggtgttc gctgcgcagt gacaggctgg gtgcatcaaa gttcacgtca
      421 agccagtcgc tcaagccata cggtattggg agcttggtga gctcagtgat tttgtgggca
      481 acaaaaccct catcgaggag cgtattgacg agccagtgtg tggcaaacag gttttgagtc
      541 ataagcgctt attgtgctgg atgcgccatg ctgacgcgct gctggctcaa ggctgccagc
      601 gacatggcgg tgatgtcgtg cgccaacggg aagtgctccg tgccacggta tatcggccga
      661 gccacaccag cgatttgcaa cgaaccagtg cggccaaacc cgacgagccg aatgcggagg
      721 ttattgacga ccatgtcctc tacggtgagt ccaatgtgat ggtgcgcttt cgggtgctcg
      781 attggttgtg gaaaccggtg gggcgcatcg tgcgcttgct tattgttcat caccctgtgc
      841 gtgggacgat ctttctgctg tccatcgata cgtcgctcga acccttggag accttgcagc
      901 tttacagcta cggcttcaag atcgagctgg ggtttcgcca ggctgtccat gtcatcggca
      961 cctatgctga tcacttctgg atgctgggca tgaagccgcc gtgccggggc tcaggcgatc
     1021 agtatttgca acgaaccacc gatgcttacc gagggccatc cgcacaaagc tgcttgccta
     1081 ccatgtgcat gttcagctcg gctgtatcgc acaacggttg ctgcagcgcc tggccatcaa
     1141 tcacacagcc gaagtttggc gctgccttcg cagttggctt cgtaccatga atccggctat
     1201 gccgccctca gagttgatcg tctccaatgc acttcgctct accccgccag aatttatcgc
     1261 ggtttccgct ttggctcctg acctgacgaa atgttggaca aatatcgccg ccatgacccg
     1321 ccctttgagg gtgagcgaat ggcggtgtga attcagcggg gaaaactagg cactgtccag
     1381 cagtgagtgg tcctataaaa aatcagagtg cacttacatc agctaagact ttaggcgcat
     1441 aaatttcaga ttcggttgac tgacaacggc tacccgcttg tataagatca attgcgctga
     1501 tgctgtgggg agtatggtca atatggccaa tatgtcaagt gacctggtct ggcgctgttg
     1561 tgtaaaccaa ctgttatctg atgcaatgaa tcaagaccaa acatgcgcca atgggtcgat
     1621 agggtcaata gaagctgctg cgagctttcg agatctgtcc aggatcaggc acagccgagc
     1681 accatggcta cataaagaga aaggctgctc accatgaacg gaacgactgt gctggttgta
     1741 gatgacgccg cgtctgttcg ggcaaccgtg tccattgcat tgaaaggtgg cggttatgaa
     1801 gtaatagagg ccagcgacgg caacgatgcg cttcgcaaac cgcaaataaa gcgtgtccac
     1861 ctcatcattt ccgatgtcaa catgccgggg atggatggca tcaccttgcc taaacgcttg
     1921 cgcgagcaag ctgcaaccaa acaagggctg gtgtctgaaa tggcaatccc caccggaatg
     1981 ttgtcttctc tgaacgcgat ggaattttga tatgaacttc aaagacatga agattgccac
     2041 ccgcctcggg tttacctttg gcttggtgct agtggtcacc atcatcgccg ccgtcctgac
     2101 cttgagtaag gtcgccgaca ttcaagaaaa cctgcgtgac attgttcaag acaacaacgt
     2161 caaggttcga ttgagcaatg aaatggcagc atctacgcac atcgttgcgc gcgtcatccg
     2221 cacgatcctt ttggtgaaag aaaaggagct cagggagcaa aggcgtaaat caattgcgga
     2281 cgcgcgcgat aactacaaca aactgtgggc tgagctggaa aaaatgccgg ttagtgaagc
     2341 aggtaaggcc attcgaaaga aagtttcgga tgccaggtcg attgtcttgg aactcaataa
     2401 cagggtgatt gaattcacca tggccgacaa ggactctgag gcgaccgagc tgctactgaa
     2461 ggaagcctcc cccgccatcc aaaaaatgtt ggatgcgctc aatgagaaca ttgccttcca
     2521 gcaggctgaa acgcaaaagg tgtacgccga agccgaagcg cagtaccaat ccacccgcag
     2581 tctgctgatt gccgccaacg cgctggtggt gctgttggct gcactatcgg gctggttggt
     2641 gacgcgctcg atcacccgac ccttgcatct tgcggtacaa gcagcagacg acgtggctgc
     2701 gggcaaactc gacggcaagt tgccgatgag tggcaacgat gaaaccggtc aactgctgca
     2761 atccttcagc aaaatgcagg gcgtattggg ccagtttcag gctgctcaag ctgagatggc
     2821 acgccaacac caggctggca tgattgactt tgctatgcct gcgcaggagc ttcctggcga
     2881 ctatgcggcc atggcccgct ctaccaatga actggtgcag gcgcacattg cactcaacgc
     2941 caaagtcatt gatctggcct tgtcctattc cgagggcaaa ctcgacacct caatggagcg
     3001 tctgccgggg caaaaggcgc gcatcaccga ggcgatgaac aaggtgcagg ccatcatgca
     3061 gcaagctgct tcagcggcca ccttcaacct gcgcatccgc aacgcactcg acagcctggc
     3121 ggccaatgtg accgtctcca acgcagatgg gttattggtg cacgccacac caccagccaa
     3181 ggcactgctc aaactcatcg ctggccccgg ttttgatgcc gatgccttct acggcaacaa
     3241 actcagcagc ctatttaccc acggtcagtc tgccgctcag ttcgaccggg ccttcaaaac
     3301 cggtgaaacg gaagacatgg atgttgcagg tcgcaaattg cgcttgcagg cgcgcccggt
     3361 actcgacgcg tctggccagc ccatcggtcg ggtcactcaa tggtttgatc gcactgatga
     3421 aatcgccgcc gaggaagaag tggcggccat cgtggctgca gcgggcgcag gcgacttcag
     3481 tcagcggctg agtaccacgg ggaaaagcgg cttcctggaa atgatcagtg ttggcatgaa
     3541 tgagctaatt gccaccagcg aacagggact gggcgatgtc gctgaggtca tggcagcgtt
     3601 tgccgacggc gacctgacca aacgcattga gcgtgactat caaggtctgt ttggcaaggt
     3661 caaagacagt gtcaacacca ccgccgaaaa cctgacccgc gtgattggcg aagtgcacgc
     3721 cgcctccgat gcattattag gtgcagctaa tcaggtcagc gccacggcgc agtcactgtc
     3781 gcaggcagcc agccaacaag cagccagtgt tgaagaaact tcgtcccaaa tcgaggtaat
     3841 gtcgacttcc atcaatcaaa acagcgacaa cgctaaagtg accgatagcg tggcgaccaa
     3901 aaccagtcaa gaggccgttg aaggcggggc tgccgtcagt tcaacggtag acgctatgaa
     3961 gcagatagca ttgaagattg gtattgtgga cgatatcgct taccaaacga acctgttggc
     4021 actcaacgct gccattgaag cagctcgagc gggcgagcac ggtaaaggct ttgcggttgt
     4081 ggctgccgag gtgcgcaaac tggctgagcg cagccaggaa gctgccaaag aaattggtgg
     4141 actggccagc aattcggtaa ccactgcaga gcgtgccggg aagttgctcg atgaaatcgt
     4201 gcccagcatc cagaaaacgt cggaattggt gcaagaaatc gcgtctgaaa gcatggcgca
     4261 aatctgctcg gtagatcaaa ttggaagcgc catggggcag ctcagcaagg ccacacagca
     4321 aaacgcatca gcctccgaag aactggcggc cacttctgaa gagctgaccg gccaggccaa
     4381 gcagctccaa gacagtattg ctttcttcaa tgtcgcagac gggtcgaaca gagagtgcgc
     4441 tcgcctggag agcaccccaa agcgccgcat gacgccccct cggctcttat ccccgttgac
     4501 acctaccgcc gtgcgatctg accgaggcaa caatttcagg ccctactgat cgccgcagac
     4561 acatactggg aagagcaatg gaaagttcac cacagaagca gtcgcgcaag gtattggagc
     4621 agcaattggt gaaagagctg gaagtcatgc gcgccg
//
LOCUS       DAOSLL010000380         4656 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16373_length_4656_cov_4.761356, whole genome shotgun sequence.
ACCESSION   DAOSLL010000380 DAOSLL010000000
VERSION     DAOSLL010000380.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16373_length_4656_cov_4.761356"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PLB25_15100"
     CDS             complement(<1..318)
                     /locus_tag="PLB25_15100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42937.1"
                     /translation="MTAIWWRQSIARRMLLALLLACTALWACIYFLGLYSTHAIGSGS
                     FDRDLKGLATAMVATLDDHPPRPAMASVLSGFDHLLESYHAQKFVVDGYMAFQVWQAD
                     GTPA"
     gene            complement(315..980)
                     /locus_tag="PLB25_15105"
     CDS             complement(315..980)
                     /locus_tag="PLB25_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018058248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE42938.1"
                     /translation="MHLLLVEDDIELGTETQRALAARGFTSVWVRCARDAHSQATAPD
                     AAAFDCVLLDLGLPDSDGMALLQAWRNQGLRVPLIVLTARDALDARVTGLNSGADDYI
                     IKPVAPDELASRIHAVTRRAVGQTHARWVVGALQIDQSRREARLNGQTVPLSPREFDI
                     LVELAKRAGQVVTKHHLARALAPLGEPLQFNTLEFHVHNLRRKLESARIETLRGIGYA
                     LHP"
     gene            complement(1597..1824)
                     /locus_tag="PLB25_15110"
     CDS             complement(1597..1824)
                     /locus_tag="PLB25_15110"
                     /inference="COORDINATES: protein motif:HMM:NF014809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HOE42939.1"
                     /translation="MADLNFRGALMVVCLLIFAVSFALLLLAAWRHHRANQSDSANFH
                     DSLWVEISWTIVPCVIVLVLVWPTVRTIWVP"
     gene            complement(1817..2737)
                     /locus_tag="PLB25_15115"
     CDS             complement(1817..2737)
                     /locus_tag="PLB25_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase"
                     /protein_id="HOE42940.1"
                     /translation="MTDTQPPTLDPQAARRWQHLPGAASPWLHEEVAQRMQERLDLIK
                     CQPAHWIDWQPGRGGLQAHGLVARRYPKATCLVFESSLAGVQLATQALVAPRWNPKAW
                     LAPKTTVAQDIGAPAQMLWSNMALHMAAEPLALLAQWHQALEVDGFLMFSCLGPDSLK
                     EMRSLYQSRQWPAPAHAFTDMHDWGDMLLSVGFAEPVMDVERITLTFETPERLLQELR
                     GLGRNLYRGRFAALRGRHWQQQLCDALASQPLQLTFEVIYGHAFKPSPKLPVSAHSAI
                     TLDEMRAALQRSRGAASASQASTTIGGQHG"
     gene            2775..3497
                     /locus_tag="PLB25_15120"
     CDS             2775..3497
                     /locus_tag="PLB25_15120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HOE42941.1"
                     /translation="MFVKLISKALTTLPRLSGLADLPGQCRVCHAWPSHAVCEACVNL
                     FAQPQQRCHRCALPLPAGMRQCGACLKNPPPLDQCLAAVAYAYPWSRLIQDFKFHAEP
                     GLVRIFATLLRAAPWVEPLLDAADLVLPMPLSQQRLKQRGYNQALLLARLLNTRKTRT
                     DLLLRIKDTPAQHTLKRSLRLTALQDAFAVEPLHISALQGRHLLLVDDVMTTGASLHA
                     AARTLLAAGASRVSAVVIARTE"
     gene            3531..3998
                     /locus_tag="PLB25_15125"
     CDS             3531..3998
                     /locus_tag="PLB25_15125"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019701915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HOE42942.1"
                     /translation="MFHIVLVEPEIPPNTGNVIRLAANTGCTLHLVEPLGFSMDDKHL
                     RRAGLDYHEYATVRQHAGWQAFLATEKPSADRLFALTTRASQSVFEALFKPGDYLVFG
                     SETRGLSDLVRQSFAAGSSLKLPMLAGQRSLNLSNAVAVTVFEAWRQNGFARR"
     gene            4136..4360
                     /locus_tag="PLB25_15130"
     CDS             4136..4360
                     /locus_tag="PLB25_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOE42943.1"
                     /translation="MPIYEYACSDCGKEFEALVRSDTVPECPVCHSKELDKQLSVFAT
                     TAASASAAPVMPGPCGTCAHPGGPGACQFN"
     gene            4371..>4656
                     /locus_tag="PLB25_15135"
     CDS             4371..>4656
                     /locus_tag="PLB25_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017522511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOE42944.1"
                     /translation="MSDKAQSFIQALKLQAHPEGGYYRETYRSDGQIDVPGAGGVVPR
                     HISTGIYFLLEKDDFSAFHRIKSDEMWHFYAGQTLEVIELQNSGDLLCTRL"
BASE COUNT          893 a         1457 c         1422 g          884 t
ORIGIN      
        1 tgctggcgtg ccatcggctt gccagacctg aaacgccatg tagccatcca ccacaaattt
       61 ttgcgcgtgg taggactcaa gcaggtgatc aaacccggac agcacagatg ccatggccgg
      121 ccggggcggg tggtcgtcaa gggtggccac catggctgtt gccagtccct tcaggtcgcg
      181 gtcaaaagac ccggatccga tcgcgtgggt gctgtacaag ccaagaaaat aaatgcaggc
      241 ccaaagggca gtgcaggcca gcaacaaggc cagcaacatg cgccgcgcta tggattgccg
      301 ccaccagatg gcggtcatgg gtgcaaggcg tagccgatgc cgcgcagcgt ttcaatgcgg
      361 gcggactcca gcttgcgcct caggttgtga acgtggaatt ccagcgtgtt gaattgcagc
      421 ggctcaccca gcggggcaag cgcacgcgcc agatggtgct tggtcaccac ctggccggcc
      481 cgtttggcca gttccaccaa aatgtcgaac tcgcgcggcg atagcggtac ggtttgcccg
      541 ttcagcctgg cttcgcggcg cgactggtcg atttgcagcg ccccgacgac ccaccgggcg
      601 tgcgtctggc ccacggcccg gcgcgtgacc gcatggatgc gcgaggccag ctcatccggg
      661 gcgaccggtt tgatgatgta gtcgtcggcc cccgaattca gcccggtgac cctggcgtcg
      721 agtgcgtcgc gggcggtcag aacgatcagc ggcacgcgca aaccctggtt gcgccaggcc
      781 tgcagcaagg ccatgccgtc gctgtcgggc aggcccaggt ccagcaacac gcagtcaaac
      841 gctgctgcat cgggggccgt cgcctggctg tgtgcgtcac gcgcgcagcg cacccacacg
      901 ctggtgaacc cgcgtgcggc cagcgcccgc tgggtttccg ttcccagctc aatgtcgtct
      961 tccacaagta gcaggtgcat ggctggtggc gctttggtca aatcgtcaag tctacccgcc
     1021 gtgcccaaaa aatcggtgcc ggctgctgtt gcagcagccc gcacataggc gtggggcctt
     1081 gatccgctgc gttggccctt gcctcaacgc gcatttgccg tttcgggccg atgtggtcgg
     1141 ccggatgttt gagccggtcc agggcgacgc tgatgatgct tcgcgtggct gtaaaaacgc
     1201 gcgcagattt gacggcaaag gttgatggtt tgcataggcc gtgctggcaa acccggacat
     1261 cgcgggcctt ggccatcatg ggcagcgcaa gcacggctga ccgggtcggc cctgcctccg
     1321 ataccccggc cgtgtcatca aaacactaca aatgttgtag ctgtcaacgc tacatccata
     1381 agggatgcag gcattattct tataaattat gagattgccg gaagtctggc aagtcggctt
     1441 cgatgatttg ttgagccggg gtcaatgtcc aattcggcgt gccgttgatt gcccaaatca
     1501 gcagcaatga gcgcaatagc gatacgcgtc ccaaatcaca gaacttctga ttcggccagc
     1561 acatcctggt ggaagcgcag ccagcgaagc accaggtcac ggtacccaga tcgtcctgac
     1621 cgtgggccac accagcacca gcacaatcac gcagggcacg atggtccatg aaatctcgac
     1681 ccacagcgag tcatgaaaat tggcgctgtc agactggttt gcccggtgat gccgccacgc
     1741 cgccagcagc agcaaggcaa acgacacggc aaatatcagc aggcacacca ccatcagcgc
     1801 gccccgaaag ttcaggtcag ccatgctggc ccccgatggt ggtgctggcc tggctggcac
     1861 ttgctgcgcc cctgctgcgc tgcaaggcgg cgcgcatctc atcgagcgtg atggcgctgt
     1921 gggcgctgac cggcagcttc gggctgggtt tgaaggcgtg gccgtaaatg acctcaaaag
     1981 tcaactgcag cggttggctc gccagtgcgt cgcacaactg ctgctgccag tgccgcccgc
     2041 gcagcgcggc aaaacgcccc cggtacaggt tgcgacccag accgcgcagt tcttgcagca
     2101 agcgctcggg ggtctcaaag gtcagcgtga tgcgttccac gtccatcacg ggttcagcaa
     2161 acccgactga cagcagcatg tcgccccagt cgtgcatgtc ggtgaacgcg tgcgctggtg
     2221 ccggccactg cctggactgg tagagcgacc gcatttcctt gagcgagtcg ggtcccaggc
     2281 aggaaaacat caaaaaacca tcaacttcca gcgcctgatg ccattgggcc agcaaagcca
     2341 gtggctcggc cgccatgtgc agtgccatat tcgaccaaag catttgcgcg ggtgccccga
     2401 tatcttgcgc aacggtggtc ttgggtgcca gccaggcttt ggggttccag cgtggcgcca
     2461 ccagcgcctg ggtggcaagc tgcacgccag ccaaagatga ctcgaacacc aggcaagtcg
     2521 cttttgggta acgccgcgcc accaggccat gcgcctgcag gccaccgcga ccgggctgcc
     2581 aatcaatcca atgcgcaggc tggcacttga tcaagtccag ccgctcctgc atgcgctgcg
     2641 ccacctcttc atgcagccac ggcgaagcag cgcccggcaa atgctgccaa cgccgggcgg
     2701 cttgcgggtc aagggttggt ggttgggtgt cagtcatggc aaaaatctgg tgtgagcgcg
     2761 agtatattga acgcatgttt gtcaaactga tctccaaggc cttgaccaca ttgccgcgcc
     2821 tgtccgggct ggccgatctg ccaggtcagt gccgggtctg ccacgcctgg ccttcgcacg
     2881 ccgtgtgcga ggcttgcgtc aacctgtttg cccagccgca gcagcgctgt catcgttgcg
     2941 cgctgccctt gccggcgggc atgcgccagt gcggtgcctg cctgaaaaat ccgccgccac
     3001 tcgaccagtg cctggccgcc gtggcttatg cctacccctg gtcacggctg attcaagact
     3061 tcaagtttca tgccgagccg ggtctggtgc gcatttttgc caccctgctg cgcgctgcgc
     3121 cctgggtcga gccgctgctt gatgcggctg atctggtgtt gccgatgccg ctgtcgcaac
     3181 agcggctgaa acagcgcggc tacaaccagg ccctgttgct ggcgcgcctg ctcaacactc
     3241 gcaaaacccg caccgacctg ctgctgcgca tcaaggacac accggcgcag cacaccctca
     3301 agcgcagctt gcgcctgacg gcgctgcagg acgcgtttgc cgttgaacca ctgcacatca
     3361 gcgcgctgca aggccgccat ctgctgctgg tggacgatgt gatgaccacc ggcgcatcac
     3421 tgcacgccgc cgcacggaca cttttggcag ccggtgccag ccgggtcagc gcggtggtga
     3481 tcgcgcgaac cgagtgagcc tgccactcgc gcgtgcacga caatttggcc atgttccata
     3541 ttgttctggt cgagcccgaa attccgccca acactggcaa tgtcatccgg ctggctgcca
     3601 acactggctg caccttgcat ctggttgagc cgctgggttt ttcgatggac gacaaacacc
     3661 tgcgccgcgc cgggctggat taccacgagt acgccacggt caggcagcat gccggctggc
     3721 aggcttttct ggcgaccgaa aaaccatctg ccgaccgcct gtttgcgttg accacgcggg
     3781 ccagccagag cgtcttcgag gcccttttca agcctggtga ctacctggtg tttggctcgg
     3841 aaaccagggg cttgagcgac ctggtgcgcc agtcgtttgc cgcaggcagc agcctgaagc
     3901 tgccgatgct ggcaggccag cgcagcctga acctgtccaa tgcggtggcg gtgacggtgt
     3961 ttgaagcctg gcgacaaaat gggtttgcgc gccggtgacg gttgataatt cagctttgat
     4021 gccatttcca gatcattcgt ggtttcaggc agcctggcgc aggcattgca aacgcaaggc
     4081 caggccagtc gacaacgacc tggatgattt ggaaatcaaa taacctggag ttgacatgcc
     4141 gatttatgaa tacgcctgca gtgactgtgg caaggagttc gaagcactgg ttcgttccga
     4201 cacggtcccc gaatgccccg tttgccattc aaaagaactc gacaaacagc tttcggtctt
     4261 tgccaccacg gccgcatcag ccagcgccgc gccggtcatg cccggcccat gcggcacctg
     4321 tgcccatccc ggcgggccgg gtgcctgcca attcaactga cccagtgccg atgtctgaca
     4381 aggcccagag tttcatccag gcactcaagc tgcaagccca cccggaaggc ggctactacc
     4441 gggaaactta ccgcagcgac ggtcagatcg acgtgccggg ggcaggcggg gtcgtgccaa
     4501 gacacatcag caccgggatt tattttttgc tggagaaaga tgacttttca gcgtttcacc
     4561 gcatcaagtc agatgaaatg tggcacttct acgctggcca gacgcttgaa gtcatcgaac
     4621 tgcaaaattc tggtgatttg ctgtgtaccc gtttgg
//
LOCUS       DAOSLL010000381         4653 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16386_length_4653_cov_4.619182, whole genome shotgun sequence.
ACCESSION   DAOSLL010000381 DAOSLL010000000
VERSION     DAOSLL010000381.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16386_length_4653_cov_4.619182"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="PLB25_15140"
     CDS             <1..343
                     /locus_tag="PLB25_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HOE42945.1"
                     /translation="DVFEKDNLLARSRSLGVHLTASLRAMAETDKRIADVRGLGAMVA
                     IELCKGGDVNQPDADLAKALATEATKRGLILLTCGTYGNVVRILVPLTASETIVNEGL
                     SILQASLAALA"
     gene            423..1508
                     /locus_tag="PLB25_15145"
     CDS             423..1508
                     /locus_tag="PLB25_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE42946.1"
                     /translation="MATTEPLVRFSGVQKSYDGSTLVVRDLNLDIQSGEFLSLLGPSG
                     SGKTTTLMMLAGFESPTSGEIFLAGKPITKTPPHRRNFGMVFQNYALFPHMTIADNVA
                     YPLTVRKVARAERQTRVTKALDMVHLGGKGERYPGQLSGGQQQRVALARALVFNPQLV
                     LMDEPLGALDKQLREHMQIELKELHRKLGLTFVYVTHDQSEALTMSDRVAVFNDGAIQ
                     QIDAVTQLYETPVNRFVAGFVGDNTVFDGVLSASEGQQCSVQFSGGSHFTGVNVNAAT
                     PGSKVQACIRPERIHAHFAADRPSSNCLQATVDDVIYFGDHLRLRCKVADQLEATVKI
                     ALASQAVPQPGDLVWLHLPPEHLRIYL"
     gene            1563..>2004
                     /locus_tag="PLB25_15150"
     CDS             1563..>2004
                     /locus_tag="PLB25_15150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE42947.1"
                     /translation="MKLKPIVAACALFASLAAVAATEITVVNFGGANGNAQKIAYFAP
                     YEKATGNKVVGVEYNGEQAKIKAMVEAKKVNWDVVEVESPDVTRGCDEGLFEKLDYQK
                     IGNKADFQPAAVHECGIGTFVWSTVMAYDGDKLKTAPTTWADFWD"
     assembly_gap    2005..2014
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2015..2596
                     /locus_tag="PLB25_15155"
                     /pseudo
     CDS             2015..2596
                     /locus_tag="PLB25_15155"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            2620..3861
                     /locus_tag="PLB25_15160"
     CDS             2620..3861
                     /locus_tag="PLB25_15160"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE42948.1"
                     /translation="MTLASTAVPDNLAAQLWRSQRRKKALAISLTLPLLIFLLATFIV
                     PIGALLIRAVENPEVASTLSRTVAALDNWDKTSSPPDAAYAALAADLANIAEQSDAGG
                     LARRLNSEISGARSLVMSTYRALPFDAGLSPAQVKARLLALDPRWNEPGYWQAIAKNG
                     SRWTPDYLLTSVDLKRDMQGNIAKVDADSAAFSKILLRTFSISAVVTLFCLLLGYPLA
                     YWLSTLTPRQANILMILVLVPFWTSILVRSAAWIVLLQSNGLVNRSLLELGFISEPLP
                     LLFNRLGVIIAMVHILLPFMILPLYSVMKSVPASFLRAAVSLGSSPLAAFFRVYVPQT
                     YPGIGAGGLLVFILSIGYYVTPALLGGADDQMLSYYIAQYTNVNVNWGMACALGAVLL
                     SATLVLYAVYRRVVKSELSMG"
     gene            4199..>4653
                     /locus_tag="PLB25_15165"
     CDS             4199..>4653
                     /locus_tag="PLB25_15165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE42949.1"
                     /translation="MSLPARPMFPAYATLPDKIGWYVLRGTGMLVLLFLLLPILVIMP
                     LSFSSSSFLSYPMPGWSLQWYDNLFSSDDWARATRNSFIVAPLATILATILGTMAAVG
                     LARIQFWGKGLLMSLLIAPMVVPIVVVGVSTYLFFARIGINDTYLGLVLV"
BASE COUNT          883 a         1390 c         1375 g          995 t
ORIGIN      
        1 tgatgtgttt gagaaggaca acttgctggc acgcagccgc agcctgggcg tgcacctgac
       61 ggccagcctg agggccatgg cggaaacaga caagcgcatt gccgacgtgc gcggtttggg
      121 cgcgatggtg gccattgagc tatgcaaggg tggtgatgtg aaccagcctg atgccgacct
      181 ggccaaagcc ttggcgaccg aagccaccaa acgcggcttg attctgttga cctgcggcac
      241 ctatggcaat gtggtgcgca tcctggtgcc actgacggca agcgagacca tcgtgaacga
      301 gggcttgagc attctgcagg ccagtctcgc agcgcttgcc tgactggcac gttatttgcg
      361 cagatgatgg ctgccagcct ttgtcctgtc tgcattggct gttctttgtg aaatagcaaa
      421 caatggcaac cactgaaccc ctggtgcgct ttagcggcgt gcaaaagtcc tacgatggca
      481 gcaccctggt ggtgcgcgac ctgaacctcg acatccagag cggcgaattc ctgtcgctgc
      541 tcgggccctc aggttcaggc aaaaccacca cgctgatgat gctggccggg tttgagtcgc
      601 ccacttctgg cgagatcttt ctggcgggca aacccatcac caagacaccg ccccacaggc
      661 gcaactttgg catggtgttt cagaactacg cactgtttcc gcacatgacg attgccgaca
      721 acgtggctta cccgcttacg gtgcgcaagg tggccagggc cgagcgccag acccgcgtca
      781 ccaaggccct cgacatggtg catctgggcg gcaagggcga gcgctaccct ggccaactct
      841 ctggcggtca gcagcagcgc gtggcactgg cccgtgcgct ggtgttcaac ccgcaactgg
      901 tgttgatgga tgagccgctc ggggctctgg acaagcaact gcgcgaacac atgcagatcg
      961 aattgaaaga actgcatcgc aagctcggtc tgacctttgt ctatgtcacg cacgaccagt
     1021 ccgaggcgtt aacgatgtct gaccgggtgg ccgtgttcaa cgacggtgcg atacagcaaa
     1081 ttgatgccgt gacacagttg tatgaaacac ccgtcaaccg ttttgttgct ggttttgtcg
     1141 gcgacaacac ggtgtttgac ggcgtgctga gcgccagcga gggccagcag tgcagcgtgc
     1201 agttttcggg cggcagtcat ttcaccggtg tcaatgtcaa cgcggctaca ccgggcagca
     1261 aggtgcaggc ctgtattcgc cctgagcgca ttcacgccca ctttgccgct gaccgcccca
     1321 gcagcaactg cctgcaggcc acggtggacg acgtgattta ttttggcgac cacctgcgcc
     1381 tgcgctgcaa ggtggccgat cagcttgagg ctactgtcaa gattgcgctg gccagccagg
     1441 cggtgcccca gccgggtgat ctggtctggc tgcacttgcc gccagagcac ttgcggattt
     1501 acctttgaac cacatgcatt ttttcctgtc cctgtcttta ctctcataaa tggagcttgc
     1561 tgatgaaact caaaccgatc gtggccgcct gtgccctgtt tgcctctctt gccgctgtcg
     1621 cagcgaccga gatcaccgtg gtcaacttcg gcggtgccaa tggcaacgcc caaaagatcg
     1681 cctactttgc gccctatgag aaggccaccg gcaacaaggt ggtgggggtg gaatacaacg
     1741 gtgagcaagc caagatcaag gccatggtag aggccaaaaa ggtcaactgg gacgtggtcg
     1801 aggtcgagtc ccccgatgtg acccgtggct gcgatgaagg cctgttcgag aagcttgact
     1861 atcaaaaaat aggcaacaag gccgactttc aaccggctgc cgtgcatgag tgcggcatcg
     1921 gcacctttgt ctggtccacc gtgatggcct acgacggcga caagctcaag actgccccga
     1981 ccacctgggc cgatttttgg gacgnnnnnn nnnnatttgc cggcaaacgt gccatgcgca
     2041 agggtgcccg ttacaacgtc gaatttgccc tgatggccga tggcgtgccg gttgccgacg
     2101 tgtacaaggt gctgatgacc aaagagggcg ctgaccgcgc gttcaagaag ctcacagaac
     2161 tcaagcccaa cattcagtgg tgggaagctg gtgcgcaacc accgcagttt ctggtggcag
     2221 gcgacgtggt catgaccacc gcctacaacg gccgtatcga tgccgcccag cgcgaggcaa
     2281 aaacctcaaa atcacctgga ccggcggtat ttatgacctg gactactggg tcatgcccaa
     2341 aggcacgccc aacaaggaag ccgccctgaa gttcatcgcc atggccagca gtgctgatgc
     2401 gcaagccgag tacgccaaga acatttcgta tggcccgacc aacaacaagg cgctggccaa
     2461 gctcgatgcc aaggtgctcg cgcaactgcc gacttctgcg gccaacgcca aaaccgcgct
     2521 gcagttcaac gtgagcttct gggctgatca gggtgaagcg cttgagaagc gttttgccgc
     2581 ctgggcggcc caataagttg atctgaagca gggcaggcaa tgacgctcgc ttcgactgcg
     2641 gttcctgaca acctggcggc gcagttgtgg cgttcgcaac gccgcaaaaa ggccctggcc
     2701 atctctttga cgctgccttt gctgatcttt ctgctggcca cctttatcgt gccgattggc
     2761 gcgctattga ttcgcgccgt ggaaaacccg gaagtggcca gcacactgag tcgcaccgtg
     2821 gcagccttgg acaactggga caaaacctcc agtccgcccg atgccgccta cgcggccttg
     2881 gccgccgacc ttgccaacat tgccgagcag tctgatgccg gcggcctggc gcgccgcctc
     2941 aacagcgaaa tcagcggtgc ccgctcgctg gtgatgagca cttaccgcgc cttgccgttt
     3001 gacgcggggc tgagcccggc ccaggtgaag gcccgcctgc tggcgctgga cccacgctgg
     3061 aacgagcccg gctactggca ggcgattgcc aaaaatggtt cacgctggac gcctgactat
     3121 ttgctgacct cggttgacct gaagcgcgac atgcagggca acattgccaa agtcgatgcc
     3181 gattcagccg ctttcagcaa aatcctgctg cgcaccttca gcatcagcgc cgtggtcacc
     3241 cttttttgcc tgctgttggg ctacccgctg gcctactggc tgtcaacgct gaccccgcgc
     3301 caggccaaca ttctgatgat tctggtgctg gtgccgttct ggacctcgat cctggtacgt
     3361 tcggcggcct ggatcgtgct gctgcaatcc aatgggctgg tcaaccggtc gctgctggag
     3421 ctgggcttca tcagcgaacc attgccactg ctgtttaacc ggctgggggt gatcattgcc
     3481 atggtgcaca tcctgctgcc cttcatgatc ttgccgttgt acagtgtgat gaagtcggtg
     3541 ccggcgagct tcctgcgcgc tgccgtgtcg ctcggctcgt cgccgctggc ggcatttttt
     3601 cgggtttatg tgccgcaaac ctatcccggc attggcgctg gcggcctgct ggtgttcatc
     3661 ttgagcattg gctattacgt gacgccggcg ctgttgggcg gtgccgacga ccagatgctg
     3721 agctactaca tcgcccagta caccaatgtc aacgttaact ggggcatggc ctgcgccctg
     3781 ggtgcggtgc tgctgtcggc cacactggtg ctgtacgcgg tgtaccgccg ggtcgtcaag
     3841 tccgagttga gcatgggcta aagacatggt catcaataac tgccccattt ggcgcgtcag
     3901 gtttgggcgc actgcgccgt tgcaaagcgc cgatgtggtt cgccacatgt cgcgctctgc
     3961 acttggcatt gcatcccaaa tccacccgcc caatgcgtca cctaggttcg accacgtcct
     4021 aagccggaca agccggaatc gaacagcctt cgtcgcgcga cacccgcaga acatggtcgg
     4081 cgcattttga atttgtggga gttgcgccct cgcgacgaat gacttcatga ctttcttgaa
     4141 ttcttctcgg aaattgcaaa gagttgcctt tttagcctaa agccagggaa ccaacatcat
     4201 gtcactgcct gcccgcccca tgtttccggc ctacgccacc ttgcccgaca agattggctg
     4261 gtatgtgctg cgcggcactg gcatgctggt gctgctgttt ttgttgctgc cgattctggt
     4321 catcatgccg ctgtcattct caagcagttc gtttttgtca tacccgatgc cgggctggtc
     4381 gctgcagtgg tacgacaacc tgttttcgtc ggacgattgg gcgcgtgcca cccgcaacag
     4441 ctttatcgtg gcaccgttgg ccaccatcct ggccaccatc ctgggcacca tggcggcggt
     4501 cggtctggct cgcatccaat tctggggcaa aggcttgctg atgagcctgc tgattgcgcc
     4561 aatggtggtg ccgattgtgg tggtgggtgt cagcacctac ctgttttttg cccgcattgg
     4621 catcaacgac acctacctgg ggctggtgct ggt
//
LOCUS       DAOSLL010000382         4648 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16410_length_4648_cov_4.598519, whole genome shotgun sequence.
ACCESSION   DAOSLL010000382 DAOSLL010000000
VERSION     DAOSLL010000382.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4648)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4648
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16410_length_4648_cov_4.598519"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..191)
                     /locus_tag="PLB25_15170"
     tRNA            complement(116..191)
                     /locus_tag="PLB25_15170"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(153..155),aa:Pro,seq:ggg)
     gene            complement(296..1948)
                     /locus_tag="PLB25_15175"
     CDS             complement(296..1948)
                     /locus_tag="PLB25_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HOE42950.1"
                     /translation="MVAPPAAIAVNQQFHWIDRLAHVLLALVAIILLYFVALPLAAIL
                     VQALQSGSGEFIWLANFVAYAQTPALLGSLWNSLWVSGLVTLIVLPLAFTFAYALSRS
                     CMPYKALFRGITLVPLLAPSLLAAISLIYWFGNQGVLKGWLQSIGFDEIYGAPGVVLA
                     ECIAVFPHTLMILVTALSMSDARLFEAADAMGTTAVRKFFTITLPGAKYGLISAAMVT
                     FTLVITDFGIPKVIGGNFNVLATDVFKLVIGQQDFAKGAVVAILLLTPAVLSFAVDNY
                     VSRRQTAMLTARAIPYSAKPSPLFDRLMTLYCAIIALLVLAMLGMAVFASFATFWPYN
                     LTPSLRHYTLGLLDAEVGVAFFNSLKMAAGTALFGTALVFVGAYLLEKTKGMDGLRAL
                     VRLLAVIPMAVPGLVLGLGYIFFFNAPANPLGGLYHTLTLLTVCTIVHFYTTGHLTAV
                     TALKALDSEFESVSASLKVPFYKTFWRVTLPVCTPALVDMARYFFINAMTTISAVVFL
                     YSPETKVASIAILNLDEAGEMGAAAAMARGAASARLFPCRRP"
     gene            complement(1948..2991)
                     /locus_tag="PLB25_15180"
     CDS             complement(1948..2991)
                     /locus_tag="PLB25_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HOE42951.1"
                     /translation="MFLDLRQIRKAFGSFTALQDINLGIQKGEFVCFLGPSGCGKTTL
                     LRIIAGLELQTAGQVFQAGRDISLLPPALRDYGIVFQSYALFPNLSVFDNVSYGLVNR
                     KTPRAEIRQRVTDLVKLVGLPGSEAKFPGQLSGGQQQRIALARALATSPGLLLIDEPL
                     SALDALERVRLRQEIRSLQQELGVTTIMVTHDQEEALSLADRIVVMNHGVIEQVGTPV
                     DIYREPASPFVADFVGKVNVLGANRVNGRLQLGRFSLPGHAHNNGPVKAYLRPEDVLA
                     QPIAPGDAHVFDARIEKIDFLGAYCHVVVSSWSLDEHRLTVYLALNFLSEQSLQVGSQ
                     LQLKLLPERIKVF"
     gene            complement(3020..4060)
                     /locus_tag="PLB25_15185"
     CDS             complement(3020..4060)
                     /locus_tag="PLB25_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_function: GO:0033226 - 2-aminoethylphosphonate binding
                     [Evidence IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOE42952.1"
                     /translation="MRLSKFAKTLALAGLFAAFGAASAEKTQLLVYTALETDQLKAYQ
                     EGFNKTNPDIEIKWVRDSTGVITAKLLAEKANPQADAVMGVAASSLALLDKQGMLIPY
                     APLNLDAIMSQYRDKKTPPAWFGMDVWGATVCFNTVEAQKKNIPKPESWQDLTKPVYK
                     GQVVMPNPASSGTGYFDVVAWLTLFGDQAGKGGGWKFMDGLHANIAQYTHSGSKPCNM
                     AASGEFVVGISFEYRANANKAKGAPIDLVFPKEGLGWDLEAFAIHKGTKKLDAARKLA
                     DWASSKDAMLLYGKNFAITAQPGVAAPLANVPKDYEQRLVKLDFNYAADQRERILAEW
                     TRRYDGKTEQKK"
     gene            4246..>4648
                     /locus_tag="PLB25_15190"
     CDS             4246..>4648
                     /locus_tag="PLB25_15190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOE42953.1"
                     /translation="MNALLHPTIALLQSHSLASAAQQAIEQGILSGEYPPGAKLNEVT
                     LADKFGVSRGPIREAFRMLDEAGLVRTEKNRGVFVRDISVIDATEIFELRAVMEEWVG
                     RQLASHITQTQSDEISTLLQAMTQAIADQQVD"
BASE COUNT         1038 a         1358 c         1356 g          896 t
ORIGIN      
        1 gacgggagat cttggagtgc gtcagtttga accgcctcct gaccgacaca agtcttgtgc
       61 tggtaaagag aaggccgttc gactttctcg tcgaacggcc tcttctgaac aaaagtcggg
      121 gcggagggat ttgaaccctc gacctcttga cccccagtca agcacgctaa accaagctgc
      181 gccacgcccc gggctttgcg aaaacggtct ggcggcctgg atgggcgtct gtctgatcac
      241 gcacccaagc tggcctaggc agctttcgcc gctggtggac ggcattatat tcattctacg
      301 gacgcctgca agggaatagc cgggcagatg cagctcctcg cgccatggcg gcggcggccc
      361 ccatttcgcc agcttcgtcg aggttcagga tggcaatcga agccaccttg gtctcgggcg
      421 agtataaaaa caccacagcc gagatggtgg tcatggcgtt gatgaaaaag taacgcgcca
      481 tgtccaccag cgcgggcgtg cataccggca gcgtcacgcg ccagaaggtc ttgtaaaacg
      541 gcactttcaa ggaggcgctg acggactcaa actcgctgtc caacgctttt aatgcagtca
      601 ccgccgtcag atgcccggtg gtgtaaaagt ggacgatggt gcaaacggtc agcagggtca
      661 gcgtgtggta cagcccgccc agcgggttgg ctggcgcatt gaagaaaaag atatagccca
      721 ggcccagcac caggcccggc accgccatgg gaataactgc cagcagccgc accagcgcac
      781 gcaggccgtc catgcccttg gttttttcca gcagataagc cccgacaaac accagcgccg
      841 tgccaaacag ggcggtgcca gcagccattt tgaggctgtt gaaaaacgct acgccgacct
      901 cggcatccag caggcccagg gtgtaatggc gcaggctcgg ggtcaggtta tatggccaaa
      961 aagtggcaaa cgaggcaaac actgccatgc ccagcatcgc cagcaccagc aaggcgatga
     1021 tcgcgcaata caaggtcatc aaccggtcaa acagcggcga tggcttggca gagtatggaa
     1081 tggcgcgggc ggtcagcatg gcggtttggc ggcggctgac atagttatcg accgcaaaac
     1141 tcagcactgc cggggtcagc agcaaaatcg ccaccaccgc acccttggca aaatcctgct
     1201 ggccaatcac cagcttgaac acgtcggtgg ccagcacatt gaaattgccg ccaatcacct
     1261 tggggatgcc aaagtcggtg atcaccaggg taaaagtcac catggcagcc gagatcaaac
     1321 cgtatttggc acctggcagc gtgatggtga aaaacttgcg cactgccgtg gtgcccatgg
     1381 catctgccgc ttcaaacagg cgcgcatccg acattgacaa ggccgtgacc agaatcatca
     1441 gggtgtgcgg aaacaccgca atgcactcgg ccagcaccac gccgggtgcg ccgtaaattt
     1501 cgtcaaaacc gatgctttgc agccagccct tgagcacacc ctggttgcca aaccagtaaa
     1561 tcagtgaaat cgccgccagc agcgatggcg ccagcagagg caccagggtg atgccgcgaa
     1621 acagcgcctt gtacggcatg caggagcggc tcaaggcata agcaaaggtg aaggccagcg
     1681 gcagcacaat cagcgtcacc aggccagaca cccacaagct gttccacagg ctgcccagca
     1741 gggcaggggt ctgcgcgtaa gcgacaaaat tggccagcca gataaattcg ccactgccag
     1801 actgcagcgc ctggaccagg atggcagcca gcggcagcgc cacaaaatac aacaaaatga
     1861 tagctaccag cgcaagcagg acatgggcta gacggtcaat ccaatgaaat tgctgattga
     1921 ccgcaatggc cgcaggcggg gccaccatca aaacaccttg atccgctcgg gcaagagttt
     1981 gagctgcaac tgcgagccaa cctgcagcga ctgctcggac agaaaattga gcgccagata
     2041 caccgtcagc cggtgttcgt caagcgacca ggaactcacc acgacgtgac agtaggcgcc
     2101 gaggaagtcg attttttcga tgcgcgcatc aaacacatgc gcatcgcccg gggcaattgg
     2161 ttgtgccagc acgtcctccg ggcgcaggta ggccttgacc ggaccgttgt tatgggcgtg
     2221 acccggcagg ctgaagcgac cgagttgcag ccggccgttg acgcgattgg caccaagcac
     2281 attgaccttg ccgacaaaat ctgccacaaa aggcgaggcc ggttcgcggt aaatatcaac
     2341 cggtgtgccg acctgctcga tcacgccgtg gttcatgacc acaatgcggt cagccaggct
     2401 caatgcttct tcctggtcgt gcgtcaccat gatggtggtg acacccagtt cttgctgcag
     2461 gctgcgaatt tcctggcgca ggcgcacccg ctccagtgca tccagtgccg atagcggctc
     2521 atcgatcagc agcaagcccg gcgaggtggc cagcgcccgc gccagtgcaa tgcgctgctg
     2581 ctgaccgccc gagagttgac ccggaaactt ggcttcgctg ccgggcagtc cgaccagctt
     2641 gaccagatca gtgacacgct ggcggatttc ggcacgcggt gtcttgcggt tcaccagccc
     2701 atacgagaca ttgtcaaaca cgctcaggtt gggaaacagc gcgtagctct gaaacacgat
     2761 gccgtagtcg cgcaatgctg gcggcaggag ggagatatcg cgcccggcct gaaaaacctg
     2821 cccggcggtc tgcaactcca gcccggcaat gatgcgcaac aaggtggttt tgccgcagcc
     2881 cgacggcccc agaaaacaga caaactcgcc tttttgaatg ccgaggttga tgtcttgcag
     2941 ggcggtaaag ctgccaaacg ccttgcggat ttgacgcaga tcgagaaaca agtcggtgct
     3001 cacacgctgg caaaaaaatt cacttcttct gctcggtctt gccgtcgtag cggcgcgtcc
     3061 actcggccag aatgcgctcg cgctggtcgg cagcgtaatt gaagtcgagc ttgaccaggc
     3121 gctgttcgta gtccttgggc acattggcca agggtgccgc gacaccaggt tgggcggtga
     3181 tggcaaagtt tttgccgtac aacaacatgg catccttgct cgaagcccag tctgccagct
     3241 tcctggcggc atcgagcttc ttggtgccct tgtggatggc aaaggcttcc aggtcccagc
     3301 ccagaccttc tttgggaaac accagatcaa tcggcgcgcc cttggccttg ttggcgttgg
     3361 cgcggtattc aaatgagatg cccaccacaa actcgccgct ggcggccatg ttgcagggct
     3421 tcgagccgga gtgggtgtac tgggcaatgt tggcatgcag gccgtccatg aatttccagc
     3481 cgccaccctt gccggcctgg tcgccaaaca gcgtcagcca ggccaccacg tcaaaatagc
     3541 cggtgccgct ggatgccggg ttaggcatca ccacctggcc cttgtaaacc ggcttggtca
     3601 ggtcttgcca gctctcgggc ttggggatgt tctttttttg cgcttctacc gtgttgaagc
     3661 aaactgtggc accccacacg tccatgccaa accaggctgg cggggttttc ttgtcgcggt
     3721 actggctcat gatcgcatcc agattgagcg gcgcgtaagg aatcaacatg ccctgtttgt
     3781 ccagcagtgc caggctcgac gctgccaccc ccattaccgc gtcagcttgc ggattggctt
     3841 tttcggccag cagcttggcg gtgatgacgc cggtggagtc gcgcacccac ttgatctcga
     3901 tgtccgggtt ggttttgtta aagccttcct ggtaggcctt gagctggtcg gtttccagcg
     3961 cggtatagac cagcaactgg gttttttcgg ccgaggcagc gccgaatgcg gcaaacaaac
     4021 ccgcgagggc aagagttttg gcaaatttgg acaaacgcat caagatctcc aagttagaaa
     4081 agtcgtcatg ttgtgacctg catgtgacaa agtgatgaca gtgcgaattc ttaagcaatt
     4141 cctgtacctc cagccgcctt tttaatttgg catttccttg attagtttgt caattgttat
     4201 ttgtagattg ttgacaatct atgaattcat gctccaatca ggcgcatgaa cgcgctgctt
     4261 caccccacca ttgccctgct tcaaagccat tcgctggcga gtgcggcgca acaggcgatt
     4321 gaacagggca ttttgtcggg cgagtacccg ccaggggcaa agttgaacga ggtgaccttg
     4381 gccgacaaat tcggggtgtc gcgcggcccc attcgcgagg cttttcgcat gctcgatgag
     4441 gccggcctgg ttcgcaccga aaaaaatcgc ggtgtctttg tgcgagacat ctcggtcatt
     4501 gatgccaccg agatctttga gctgcgtgcc gtcatggaag aatgggttgg gcggcaactg
     4561 gccagccaca tcacgcagac gcaatcggac gaaatcagca ccctgctgca ggccatgacg
     4621 caagccatcg ctgaccagca ggttgacg
//
LOCUS       DAOSLL010000383         4638 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16439_length_4638_cov_5.570151, whole genome shotgun sequence.
ACCESSION   DAOSLL010000383 DAOSLL010000000
VERSION     DAOSLL010000383.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16439_length_4638_cov_5.570151"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(473..706)
                     /locus_tag="PLB25_15195"
     CDS             complement(473..706)
                     /locus_tag="PLB25_15195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42954.1"
                     /translation="MDLTLGTVGELVRPPHWRRNEKADLHHAGGELLHIAKAGDGCPW
                     MMKTASRCPAVKDNQMMRANSPDQTGTAMTTTG"
     gene            811..1701
                     /locus_tag="PLB25_15200"
     CDS             811..1701
                     /locus_tag="PLB25_15200"
                     /inference="COORDINATES: protein motif:HMM:NF013103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HOE42955.1"
                     /translation="MRRFVLCHLPILVLASRYETIQRNACNTGLLLDEGRNTHRSSLD
                     ECLDWYAASKAAASLLGSCKNRLDNGVRDGKVQPRRKPRGATGYSSGLRQCMALSNAV
                     ELVRSFLLDALATLFNLMTTLQMPTFRALVSHDDERGGLTEIFRDDWAQGQVPVQWNF
                     VRTRPNTLRGVHVHVRHVDHLIVLRGTMLLGICDLREAAPAFLESSMVELSGDKLTLA
                     TIPTGIAHGFYFPEATDYVYAVTHYWDPINDELGCAWNDPGLSLDWPVRAPVLSDRDR
                     AAPPLAILLNTLKSMGVRSC"
     gene            1695..2612
                     /locus_tag="PLB25_15205"
     CDS             1695..2612
                     /locus_tag="PLB25_15205"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HOE42956.1"
                     /translation="MLNIVLLGCGRWGKLILRDLKSLGVSVAVLAHGPISQANASAGG
                     ADVVVSEFHDLPEADAYVVAVPTSLHAQYLLRLLGNNKPIFCEKPLADDVAEARRIVA
                     AAGSRLFIMDKWRYHGGVLALKSVATSGELGPVQGIKTVRVQWDSPHDDVDMDWILLP
                     HDLSIALEVLGFIPEPVTAQAEKSADGRLISLCGTLASGQTWMQFEVGIRSPSHRRLI
                     ELRCRDGIATLSDAYVSAITLLRTDNVAGVAPLPQSRPVDVDMPLLKELAAFLAYVTG
                     VGRPPRSSAADGLLIVERIADLRHIAGLQ"
     gene            2953..3897
                     /locus_tag="PLB25_15210"
     CDS             2953..3897
                     /locus_tag="PLB25_15210"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE42957.1"
                     /translation="MKLPPAFTILIPTHDHADTLWYSVQSALCQSRQDFELFIVGDGV
                     PDRTREIAAALTSRDPRIRFFDNPKGERHGEAYRHIALQQAAGQYVCYLSDDDLWFPD
                     HLEQMAKALEIHDLAHTMQIEVQPDGQLNSWMFDAMLEPDALDRMRRSETGFGLASGG
                     HSLAAYSKLPHGWHPAPKAINSDLYFWLQFFDQPECRYFSLKWPTMVHFSSVSRKSCS
                     GSQRVAELAEWWPRIQNAKERSRMVMEVLLPINAELYGLSRQPRAPVGQTVTLVQEAA
                     LKAQIEQLQVKVIALRNSLSWKLTKPLRRLADFLKAGR"
     gene            complement(4338..>4638)
                     /locus_tag="PLB25_15215"
     CDS             complement(4338..>4638)
                     /locus_tag="PLB25_15215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE42958.1"
                     /translation="QEAWDRLRAMAAEAESQGKTIKDKIALVLTDLEMPEMDGFTLTR
                     NIKQDPRFASIPVVIHSSLTGTTNEEHVRKVGADAYVAKFVAEELAATLRRVLTR"
BASE COUNT          892 a         1373 c         1353 g         1020 t
ORIGIN      
        1 cagccaatgg cggcctgaac acgaaggggg gaactacgca ctgtccagtt agatgtcaag
       61 taagaataac ttgaacccgc gtacaagcgc ggcaagctac gctgcttgtt caggcattgt
      121 gtgatgcatg cggtttcgca ttcggtttgc cctgtccccg ccgtctccga ttgaccgtgg
      181 cgacgcgtcc aaacccgatc atgcatgtct ttggccgatg cagttcaaac gcgaatcacg
      241 gcatcgattg acaagagtga gcgctttgtg cgcacggtcc agcatctgtc cagatgccga
      301 tcaatgaatg cgtcccttct tttgtgtctg gtaggttggg ctttcgttca gctttggtca
      361 atgaaggctg ccagggcggt gtcggcgcag aagattttct caagcaaatc gcctatccca
      421 tcatcgcctt tgtgttgcgc gtggggtggc gcatcgccgt catcccagcg catcagccgg
      481 tggtggtcat tgccgtgccg gtctggtcgg gactgttggc gcgcatcatc tgattgtcct
      541 ttaccgccgg acaccggctg gcggttttca tcatccaggg gcaaccgtcg ccggctttcg
      601 cgatatgcaa tagttcgccg cccgcatgat gcaaatcagc tttttcatta cgccgccaat
      661 ggggtggccg gaccagttca ccaaccgtgc ccaatgtcag gtccatcgcg acccgctggc
      721 cgcatgcctg gaaatcctgc gcaaaccctt gcctgaccaa gccgcatccg accaagcctg
      781 gcgcaccgca ttcggcctga cgacaaccct gtgcgtcgct tcgtcctgtg tcacctgccg
      841 atcctggttc tggcctctcg gtatgagaca atccagcgca acgcctgtaa taccgggctg
      901 ctcctggatg aagggcgcaa cactcaccgc agctcgcttg acgaatgtct ggattggtac
      961 gccgcgtcga aggcggccgc ttcgcttttg gggtcatgca aaaatcgctt ggacaatgga
     1021 gttcgtgatg gcaaggtcca gcccaggcgc aagccgcgcg gtgcaactgg ctacagcagc
     1081 ggtctgcgcc aatgcatggc gctttccaat gcggtcgaac tggtcaggtc atttttgctt
     1141 gatgccttag caaccctctt caatttgatg actaccctcc agatgcccac cttccgagcg
     1201 cttgtcagtc acgatgacga gcgcggcgga ctgacggaaa tcttccgcga tgattgggcg
     1261 caagggcaag tgccggttca atggaacttc gtcagaaccc ggccaaatac cttgcgtggt
     1321 gtccatgtcc atgtccgcca tgtcgatcat ctcatcgtgc tgcggggaac catgctgctc
     1381 ggcatctgcg acctgcgcga agcagcgccc gcgttcctgg agtccagcat ggtcgaactg
     1441 agcggagaca aactgacatt ggcaaccatt ccgaccggca ttgcccatgg cttttatttt
     1501 cctgaagcca ccgactacgt ttatgccgtg acgcactatt gggacccaat taacgacgaa
     1561 ttaggctgcg cctggaatga tccagggctt tcgctggatt ggccggtacg cgccccggtg
     1621 ctctcggatc gcgaccgggc tgcaccgccg ctggccattt tgttgaatac actgaaaagc
     1681 atgggagtcc gatcatgctg aacatcgttc tgttgggttg cggccgttgg ggcaagctca
     1741 ttctgcggga tctcaagtct ctgggtgtga gcgtggcggt acttgcccat ggcccgatta
     1801 gccaggccaa tgcatcggcc ggtggtgccg atgtcgtcgt ctcggagttt cacgacctac
     1861 ccgaggcgga tgcctacgtg gttgctgtgc caaccagtct tcacgcccaa tacctgttgc
     1921 gcctgctcgg caacaacaag ccgatcttct gcgaaaagcc gctcgctgac gatgtcgccg
     1981 aggcacggcg catcgtggcg gcggcgggta gccgcctgtt catcatggac aagtggcgct
     2041 accacggcgg tgtgttggcc ctcaaatctg tcgccacaag tggcgaactc gggccggtac
     2101 aggggataaa aaccgtgcgc gtgcagtggg acagcccgca cgacgatgtt gacatggact
     2161 ggatactgtt gccccacgac ctctcgattg ctcttgaagt gctgggcttt attccggaac
     2221 ctgtaaccgc ccaggccgaa aaatcggccg acgggcgact catcagcctc tgcggcacgc
     2281 tggcatccgg ccaaacctgg atgcagttcg aagtgggcat acgctcgcca tcgcatcgcc
     2341 gcctgatcga attgcgctgt cgcgacggta tcgctaccct gtcggatgct tacgttagcg
     2401 cgatcaccct gctgcgcacc gacaacgtgg cgggcgttgc gccgctgccg caatctcgtc
     2461 cggtcgatgt cgatatgccc ctgcttaagg aactggcggc cttcttggcc tacgtcaccg
     2521 gggttggccg accgccgcgc agcagcgccg ccgatggctt gctgatcgtg gaacgtatcg
     2581 ccgacttgcg gcacatcgcc ggcctgcaat gatctggatt tcctggttgg atgggcccga
     2641 gccggccgct ggcagagcgt ctgggcgggg ccttggcttc cggaggtgcc tgcgcaagtt
     2701 tggacggctt gaagcaggcc agcctggtgg catggctgcg ccccgcatgt cgccgcaagc
     2761 tgcgcgggcg acccgggctc cttgcgcgta tggcatgctg gcagacgcgg caggagcaat
     2821 gcccccggca cattgcctgc ggctcaatcg ggtgcgaagc ttggacccaa tgggtgaaca
     2881 ccatcgaaag tgaaacgtca atactggtga ggatttgcag atgaaatcaa gcggcctatt
     2941 gagggattca gggtgaagct gccgcccgca ttcacgatcc tgattccaac ccacgatcac
     3001 gccgataccc tgtggtattc ggtgcagagc gccctttgtc agagtcggca ggattttgag
     3061 ctcttcatcg ttggagatgg tgtgccagac cggacacgtg aaatcgccgc tgccctgacc
     3121 agccgcgacc cgcgaatccg gttttttgac aacccgaaag gcgaacgcca tggcgaagcc
     3181 taccggcaca ttgccctgca gcaagccgca gggcaatacg tttgctatct ttcggacgac
     3241 gacctctggt ttcccgatca tcttgagcag atggccaaag cgctggaaat ccatgatctc
     3301 gcgcacacca tgcaaatcga ggttcagccc gacggccagc tcaatagctg gatgttcgat
     3361 gccatgctgg agccggatgc gctggatcgg atgcgcagat cggaaaccgg cttcggcctt
     3421 gccagcggcg gccacagcct ggctgcttac agcaagcttc cccacggctg gcatccggca
     3481 cccaaggcaa tcaacagcga tctttacttc tggttgcaat ttttcgatca accggagtgc
     3541 cgctactttt ctctgaagtg gcccaccatg gttcactttt catccgtttc gcgcaaatca
     3601 tgtagcggca gtcagcgcgt tgcggaactc gccgagtggt ggccgagaat tcaaaatgca
     3661 aaagagcgat cgcgaatggt catggaagtc ttgcttccga ttaacgcgga gctttacggt
     3721 ttaagtcggc agcctcgagc gcctgttggc cagaccgtta cccttgtcca agaggctgcc
     3781 ctgaaagccc aaatcgagca actgcaggtc aaggtgatag cactgcgaaa cagtctgtcg
     3841 tggaaactga ccaaaccgct tcgccgattg gctgacttcc ttaaggctgg gcgttgaatg
     3901 aatgaccgaa gcgcaaagtg aactccaatg ccaaggcaaa ttgccagctc attcaggcca
     3961 tgccagtact cggacccatc tagacggcgc tgcctcggtt ttccccgttg gcattggtcc
     4021 ccatggcact gatttcaaat ggctggctgg caaacgtcat ggcatcagca actgcataca
     4081 gccccttgga tacgataact tcagtagctg cttgtccatt tcaggcgtgg gctgcagggt
     4141 gattcatgcc aaattctcag gaacgggggt ggctgcgtga tcggctggca ggatgttctc
     4201 cacgcttgcg ggcattggct tggtgtgcca ggaactcacg cgacgatatg aatcgtttac
     4261 ttctctagcc cttgtgctgc aagcgcttcc agctattcta taaatagcac tgcggcaacg
     4321 ccgctacggc ttgcgcatca gcgcgtcagc acccggcgca gggtggcggc cagttcttct
     4381 gcgacaaact tggcaacgta agcatcggca ccgaccttgc gcacatgttc ttcgttggtg
     4441 gtaccggtca gcgacgaatg gatcactacc gggatgctgg caaagcgcgg atcctgcttg
     4501 atgttgcggg tcagggtaaa gccgtccatc tcgggcattt ccaggtcggt caacaccagg
     4561 gcgattttgt ccttgatggt tttgccctgt gattcggcct cggctgccat cgctctgagc
     4621 cgatcccagg cttcctgg
//
LOCUS       DAOSLL010000384         4606 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16572_length_4606_cov_3.462536, whole genome shotgun sequence.
ACCESSION   DAOSLL010000384 DAOSLL010000000
VERSION     DAOSLL010000384.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16572_length_4606_cov_3.462536"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..2544)
                     /locus_tag="PLB25_15220"
     CDS             complement(214..2544)
                     /locus_tag="PLB25_15220"
                     /inference="COORDINATES: protein motif:HMM:NF020290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PriCT-2 domain-containing protein"
                     /protein_id="HOE42959.1"
                     /translation="MDQLIKSSLLVDSITPIESNTKPKFTLPAHLIVKPKVDGSGAKQ
                     QRDAAQSKPVDDGEQLTEALWRTRHHVHFLGERDAHSGRFNNVPVRNDLPVVQLATRL
                     AGERSDIYLAVAEYQTDAGRTQSNAVGAFAFWADIDCGEGKAAQGKGYATIDLALAAV
                     DTFCGQTGLPKPNFIINSGAGLHVYWVMVDFVAKDRWQPPARKLKALAEKMGFLADPT
                     RTADIASVMRLPGTLNRKYDPPLPVTLRQYSSDFLANSLVLEAIEHAYEKHCEGAMVN
                     PSSLGPARNPATARAAPTGPKEAARNVAKMEILLTAIDANCGYDDWRNVLMAIHHESG
                     GSAQGLDLADRWSSTGSDYPGRPEIEMKWRSFEHGSATPITMGTLNKMVSDSGVDWKE
                     LLAAAEPDFDSCETETVYPNVSASPRGVPHAQSIQPVQPVNPLAKFSLQGHVTELEKL
                     MVEQRPLLGDIVLNGQATVAFAKANTGKTLIILSLIIAAIKEGRIDPSKLIYINMDDN
                     SSGLVEKVRLADEYGFHMVADGHKGFEAKEFRAAMEDMIENNSVNGTIVVLDTLKKFV
                     NTMDKGKSSNFAKVVRQFVLKGGTVIALAHANKNPGPDGKIVHSGTTDIVDDFDCAYT
                     LFALPSQPDATEKLVEFENIKRRGNVTSTAAYAYSLERNLSYEELLLTVREVDPAQLG
                     PIRLAASLVDDAVVIAAIEACIKDGTNTKMKLAEAASKIADVSQRDALKVIEKFTGSD
                     PATHRWVFEVRERGAKVYRLLERPTTPSGSPPVATP"
     gene            complement(2872..3102)
                     /locus_tag="PLB25_15225"
     CDS             complement(2872..3102)
                     /locus_tag="PLB25_15225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42960.1"
                     /translation="MNTHSWHQLAVPPLTSLAGSPVLTSGQISQVLACLFFMLVTSNG
                     TVARCAGHTFLSEDALVWKSWTQFVATVRCQL"
     gene            complement(3190..3555)
                     /locus_tag="PLB25_15230"
     CDS             complement(3190..3555)
                     /locus_tag="PLB25_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42961.1"
                     /translation="MPQIDSHFDELTVLQRIAEVCRYLCLRLEYAVSSGGTLRAWVRV
                     NLMVLLTGIVPALLVVPLLTLLASEFTSLTGYLLQASRNLLDAVVTLIEAAAAFVAAF
                     MTLKVCANGGAAGGFKRRR"
     gene            complement(3623..3916)
                     /locus_tag="PLB25_15235"
     CDS             complement(3623..3916)
                     /locus_tag="PLB25_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42962.1"
                     /translation="MHRFIHLILVAVLLGGCGHKEEATQIAQQQVIQAEKNAAIALAN
                     LKVAEEKAALEKAVSQRIIFALGVGALLALFVGIGLGSSARRTADRIRRVSDA"
     gene            complement(4092..4508)
                     /locus_tag="PLB25_15240"
     CDS             complement(4092..4508)
                     /locus_tag="PLB25_15240"
                     /inference="COORDINATES: protein motif:HMM:NF015205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HOE42963.1"
                     /translation="MITLANIHSERLDDTLRVAFTAKLFGGHFPTKLEPKIYGMADML
                     APAYNGGYWHFYGLSNGGFYMAPATDTVFVVSCENGFDGKLSADALGITACLYAYSNL
                     SFGEGAFPQTCAQHYQLLREFSMEHAEARAILRATD"
BASE COUNT          969 a         1352 c         1305 g          980 t
ORIGIN      
        1 cccatgacgc atggcgatgg cggtgaaagt tgagccctga attgaggctt caatacgtca
       61 caaggggctg ccccaaaccc gagcgcacta gttcgggccg gaggagtctt gattgggggg
      121 aatgccgggc ggctgacgac gcgaccctgg cggggaacct gcagacctga aatcctgcga
      181 atctgcggcg ctggaccccc gccggggccc tggtcagggg gtggctacgg gcggactgcc
      241 cgaaggcgtt gtagggcgtt ccagcagcct gtagacctta gcgccacgct cgcggacttc
      301 aaagacccat cgatgggtgg cgggatcgct gccggtgaac ttttcaatga ccttgagggc
      361 gtcccgttgg ctgacgtcag cgattttgct ggcggcctct gccaacttca tcttggtgtt
      421 ggtgccatcc ttgatgcagg cttcgatggc tgcgatgacc accgcgtcat cgacaagtga
      481 agcggcgagc cttatcggcc caagttgagc gggatcaact tcgcgaaccg tcagcagcaa
      541 ttcttcatag gaaagattgc gttcgagtga gtacgcatat gccgcggtcg atgtgacatt
      601 tccacggcgc ttgatgtttt caaattcgac caacttttcc gtggcatcgg gttgcgaggg
      661 gagtgcaaag agtgtgtacg cgcaatcgaa atcgtccacg atgtccgtgg tcccagagtg
      721 gacgattttt ccatccgggc caggattttt gttggcgtga gccagcgcaa tgacggtgcc
      781 gcccttcagg acgaactgcc tgacaacctt ggcgaaattg gaactcttgc ccttgtccat
      841 ggtgttgacg aacttcttga gcgtgtccag cacgacaatg gttccgttca cgctgttgtt
      901 ttcgatcatg tcctccatcg cagcccggaa ctccttggct tcgaagccct tgtggccatc
      961 cgcaaccatg tgaaaaccat actcgtcggc aagccggacc ttctccacca gtccgctgct
     1021 gttgtcatcc atgttgatgt atatgagctt ggatggatcg attcgtccct ccttgatggc
     1081 ggcgatgatc aagctcaaga tgatcagcgt cttgcccgta ttggccttgg caaaagccac
     1141 cgtcgcctgc ccgttgagaa caatgtcccc caatagggga cgctgttcca ccatcaattt
     1201 ttcaagttca gtgacatggc cctggagcga gaacttcgcc agcggattca caggctggac
     1261 aggctgaatg ctctgggcgt gcgggacgcc ccttggagat gctgagacgt tcggataaac
     1321 cgtttcagtc tcgcacgaat caaagtccgg ttcggcggcg gccagtagct ccttccagtc
     1381 aaccccggag tcagacacca tcttgtttag ggtacccatg gttattggcg ttgctgatcc
     1441 gtgctcaaac gagcgccatt tcatttcgat ctccggcctt ccgggatagt cgctgcccgt
     1501 gctgctccac cggtcggcca gatcaagccc ttgcgcgctg ccaccggact catggtgaat
     1561 cgccatcagc acgttgcgcc agtcgtcata cccacagttc gcatcaatgg cggtaagcag
     1621 gatttccatc tttgccacat ttcgcgcggc ttccttgggc ccggttgggg cagcccttgc
     1681 cgttgccgga ttgcgagctg gacccagcga actcggattg accatagctc cttcgcaatg
     1741 tttctcgtat gcatgctcga tggcttcgag taccaacgaa ttggcgagaa aatccgagct
     1801 gtattggcgg agcgtcacgg gcaacggcgg gtcatacttg cggttcaaag tgcccggaag
     1861 gcgcatcacg ctggcgatgt cagcggtacg ggtgggatcg gccaggaagc ccattttttc
     1921 agccaaggcc tttagcttgc gtgcgggtgg ctgccatcga tcctttgcga caaagtccac
     1981 catgacccag tagacatgaa gccccgcgcc gctgttgatg atgaagttcg gcttgggcaa
     2041 cccggtctgg ccgcagaacg tatcgacagc ggccaaggcc aagtcaatgg tggcgtagcc
     2101 cttgccctgt gcggcctttc cttcaccgca atcgatgtcg gcccaaaacg caaacgcacc
     2161 gactgcgttg gactgggtgc gccctgcatc ggtttggtac tccgcgacgg cgagatagat
     2221 atcggatcgt tcacctgcaa gtcgcgtggc caactggacg actgggagat cattcctgac
     2281 cggcacattg ttgaagcggc cgctatgcgc gtcacgttca ccaagaaaat gaacgtgatg
     2341 gcgtgttcgc caaagcgcct ctgtcaattg ttcgccgtcg tcaactggct ttgactgagc
     2401 ggcgtcgcgc tgttgtttgg ccccagaacc gtcaacctta ggtttgacaa tcaggtgcgc
     2461 cgggagggtg aatttcggtt tggtgtttga ctcaatcggg gtgatcgagt ccacaagaag
     2521 tgaagatttg attaactgat ccataggccc ccaaagtgga aaaggacgtg acttgtcgtc
     2581 ggcataaagc cgacccgtct ttccctgctc aggaaactcc tgaacagcgc gacctgggat
     2641 tgctccaggc cgcccttccc actagggtct gcaaggtcga tggcctctag tgccaccttc
     2701 caaaattctc cgatttcccg aaaccggacc gacgacgcta tctggtccgc tggggcccag
     2761 cttcacgtcc tcgtgctgaa aattcgaagc gcgattctgc cagaccttcc gaagcggtgt
     2821 cagggtgatt tgaacggcag cttctcggcc gtatgcctga gtccgacagc atcacaactg
     2881 acacctcacg gtcgccacga actgcgtcca cgatttccaa accaaagcat cttcagaaag
     2941 aaaagtatgc ccagcacaac gggccaccgt gccattcgac gtgaccaaca tgaagaacag
     3001 gcacgccaat acttgactaa tttgaccgga agttagaaca ggcgaccctg ctagcgatgt
     3061 cagcggcggc accgccagtt ggtgccagct gtgggtattc atggccattc tcttttcgga
     3121 caatctgatg ccgtctccca gcccgcccgc gttgctcttt ggccttcgaa ggctgccttt
     3181 gttgcgtcct caccgtcgcc tcttgaaccc gcctgccgca cctccattcg cacagacctt
     3241 cagggtcatg aaggccgcga caaacgccgc cgccgcctcg atcaatgtca cgaccgcatc
     3301 gagtagattg cgggacgcct gcagcagata gccggtcaat gacgtgaact cactcgccag
     3361 caaggtcagc aaaggcacca ccagcaaggc cggaacgatg cccgtcagca acaccatcag
     3421 gttcacccgc acccaggccc gcaaggtgcc acctgatgat accgcgtact ccaggcgcag
     3481 gcacagatat cggcagacct ccgcgatcct ctgcagcacc gtcaattcat caaagtgcga
     3541 atcaatctgc ggcatgggga tcggcgtcgg cgtccacgct gcgagaaggc gagcaaacag
     3601 ggatgaccga ttcaacagcc catcaggcat ctgaaacccg acgaatgcga tcggcggtcc
     3661 ttcgggccga agaacccagc cctattccga caaacagcgc cagtaaagcg cccacaccca
     3721 acgcgaagat gatgcgctgg gacacagcct tctccaatgc agctttctcc tcggcaacct
     3781 tcaggttggc caaggcgatg gctgcatttt tttcggcctg aatgacctgc tgctgggcga
     3841 tctgggtggc ttcctctttg tggccacaac cgccaaggag aacggccaca aggatcaagt
     3901 gaatgaaacg gtgcatgatt gcctccgatt gataaatgaa gaaagggcgc tgcgctggtg
     3961 ggggcagctc cgggaatcag ggccgctgaa aacctgaaat cctgcgatcc tgaaaagctg
     4021 gcgcggtccc gccccgcagc ccggcaggtt tccaggattt cagaattgca gctccgcagc
     4081 ccccacccgt gtcagtccgt ggcccgcaag atggcacgtg cttcggcgtg ttccatggag
     4141 aactcgcgta acagttggta gtgctgggca caggtctggg ggaacgcacc ttcaccaaac
     4201 gacagattgc tgtacgcata cagacatgcc gtgatgccca acgcgtctgc actcagcttt
     4261 ccatcgaagc cgttttcgca actgaccaca aagacggtgt ccgtggccgg agccatgtag
     4321 aagccaccat tgctgaggcc atagaaatgc cagtaaccac cgttgtaagc aggtgccagc
     4381 atgtcagcca ttccataaat ttttggttcc agtttcgtgg ggaagtgacc gccaaagagc
     4441 ttggcggtaa aggcaacgcg cagggtgtcg tccaggcgtt cggagtgaat gtttgcgagc
     4501 gtgatcatgt ggatgtgttc ctgaaaaatg agccccccac ggcctccctg accggttgac
     4561 taaaagctga aagcgcagag acagcgtttc tctatcgatg cgcgat
//
LOCUS       DAOSLL010000385         4605 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16573_length_4605_cov_3.985275, whole genome shotgun sequence.
ACCESSION   DAOSLL010000385 DAOSLL010000000
VERSION     DAOSLL010000385.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16573_length_4605_cov_3.985275"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1498
                     /locus_tag="PLB25_15245"
     CDS             <1..1498
                     /locus_tag="PLB25_15245"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOE42964.1"
                     /translation="ASPSLLVMSSSTQNGIEPQDVMALVFSNTTCNADLLSQVEAASF
                     ACIGEDAMNFEFNAHLLVKEIHKTMPDGRLKIIDLNWSPIADESGTTLRLMLSLRDVT
                     ELRVLAAKASEQKRELTIIGEILAVKQEKFQAFVDSAKNLIAENRALIARSDEQGNTL
                     NDPALVNLLFRNMHTVKGNARTYGLLLLTNVFHEAEQSYDALRKDPDAALDSEQLLAE
                     LAAAASSLDEYAHVSEVKLGRKGPGRRGGVDKFLMVQKADVQRDLDLLKQVDETSLAA
                     MQDVVSRLRSSLQLIGTEKIQDVLAGVLDSVPSLARELGKEVPQLVVSDHGIVVKTQV
                     ADLLRNVFMHLYRNAMDHGIESADKRLAQDKSPVGKIALELSLDDDVFTLCLRDDGRG
                     LALGVIQAKAINRKLIDADQPISAYAIAQLIFAPGLSTAQSVTAVSGRGVGMDAVKGF
                     VESQGGSISLNLLPGKADGEHRQFETLICLPGEFAVKAMTHAEFNLIA"
     gene            1854..2582
                     /locus_tag="PLB25_15250"
     CDS             1854..2582
                     /locus_tag="PLB25_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007878483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE42965.1"
                     /translation="MIEAIAALVLGALLGGAVMAWRCQQLKQAMRAMVPGQVLQSVQV
                     QLNEALAQLQAQSDQYQGREQEILGEATELSRNLDEHQQLLTQIQANFSAVNGQVLVE
                     CEAFGSGVGNLLGLVKTFERWHADMNNLIIHNREMHGKNDDFSAIVRQMVIVTLNASI
                     EAARAGEQGRGFSVVADEMRALAGRAEKLSADYRKNLYQNDLITVNTFQDLQAGGKMI
                     IGSLVGLEQTFRNIRERLSAEPSP"
     gene            2579..3133
                     /locus_tag="PLB25_15255"
     CDS             2579..3133
                     /locus_tag="PLB25_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017451185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42966.1"
                     /translation="MISNNACESFDHLLLQGIRSCLVNQDSDSCDISVQDDLTGSHES
                     HLAVLTISSYLFRLMVLIHFSADEPTREHLAKLSNASVNDLNEQAFLDAICERANMCC
                     GSLSRDLSQTFPHIGMSTPNIVDRQCASHLHSLNPGHKRHFKVTVNSTAQFHVTLCVS
                     SYDKLDFVVDKCAAEVHGGELEMF"
     gene            3166..4140
                     /locus_tag="PLB25_15260"
     CDS             3166..4140
                     /locus_tag="PLB25_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006495102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HOE42967.1"
                     /translation="MNEEIKVVSKFLVLDDDHSCHQRIKSFCDDNSLVPLKVQADNVM
                     SVLKSKVDLGGILLSENFGADAGEGIRLGHLIHAVRPELPLFLRRENCENLDDLSPPD
                     QRIFAAAYTISNMAHLRAALDESIFSFVYPNVLVRGISELTSAALESQFIDMKAEVEA
                     PYIVRDKLIFGEIFTLIPIENNWCRGYMMLQSEERSLLELVKGDRTHVSADKADDFRS
                     LNSVLGEITNLIWGGFKSRYSGAASADRNLSQVPIIINHLHRYISFGSDNPQLCFRYT
                     LTDRAHVQARSLVINQKFVFNLNWSPDDFSENVTSVEDLVSAGELDLF"
     gene            4155..4523
                     /locus_tag="PLB25_15265"
     CDS             4155..4523
                     /locus_tag="PLB25_15265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016833860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE42968.1"
                     /translation="MAQILVVDDSSTVRNEVGDFLTKSGMTVTLAVDGRDGLAKLKSD
                     SSIKLVVCDVNMPNMDGLTMAEKVRSELHNQSVNIIMLTTENSPAMKERGKAAGIKGW
                     IVKPFKGDAVLATFKKLVGA"
BASE COUNT         1076 a         1155 c         1287 g         1087 t
ORIGIN      
        1 tgctagtcca tcgctgcttg tgatgtcaag ttccacacaa aacggcatag agccccagga
       61 tgtcatggca ctggtttttt ccaataccac ctgcaatgca gatttgctgt cgcaagtgga
      121 ggctgccagc ttcgcttgca tcggtgaaga tgcgatgaac tttgaattca atgctcactt
      181 gctggtcaag gaaattcaca aaaccatgcc agatggtcgc ctcaagatca ttgatttgaa
      241 ctggtcaccg attgctgatg aatcaggcac aacactgcgc ctgatgttga gcctgcgtga
      301 tgtcaccgag ctgcgcgtcc tggcagccaa ggccagcgaa caaaaacgtg aattgaccat
      361 cattggcgaa atactcgccg tcaagcagga aaagtttcaa gcttttgtag acagcgcgaa
      421 aaatctcatc gctgaaaatc gagccttgat cgcgaggagc gatgagcaag gcaacacctt
      481 gaacgaccct gcgctggtca atttgctgtt tcgcaacatg cacaccgtca agggcaatgc
      541 acgcacttac ggtttgttgc tcctgaccaa tgtgttccac gaagctgaac agtcatatga
      601 cgcgctgcgc aaagatccgg atgcggcttt ggacagtgaa caactgcttg ccgagctggc
      661 cgctgcagca tcttcgctgg acgaatatgc gcatgtcagc gaggtcaaat tgggtcgaaa
      721 agggcccggg cgtcgtggtg gggttgacaa gttcctgatg gttcaaaagg ccgatgtgca
      781 gcgggatctg gacctgctca agcaggttga cgaaaccagt cttgcagcca tgcaagatgt
      841 cgtttcgcgc ttacgaagca gcctgcaact gattggcacg gaaaaaatcc aggatgtgtt
      901 ggccggtgtg ctggactcgg tgccgtccct tgccagggaa ctgggcaagg aagttccgca
      961 acttgttgtc agcgatcacg gcatcgtggt gaagacccag gtggctgatc tgcttagaaa
     1021 cgttttcatg cacctctatc gcaatgcgat ggaccatggg atcgagtcgg ctgacaagcg
     1081 gctggcccag gataaatcgc cggtggggaa aatcgcactg gaactgtcat tggatgacga
     1141 tgtgttcaca ctgtgccttc gtgacgatgg acgtggtctg gcactgggtg tcattcaggc
     1201 caaggcgatc aatagaaaac tgatcgatgc cgatcagccg atcagtgctt acgccattgc
     1261 acaacttatt tttgctccgg gtttgtctac cgcacaaagt gtcactgcgg tgtctggcag
     1321 gggcgttggc atggatgccg tcaaaggctt tgtcgagagc cagggtggct ccatttcact
     1381 caacttgctg cccggcaagg ccgatggtga acatcgccag ttcgagaccc ttatttgtct
     1441 gcccggcgag tttgcggtca aggccatgac gcatgctgaa ttcaatctca ttgcatgaac
     1501 cttgattcct cagttgaccg cttgttttca tctgtaagtt cttatgatca tttacttttt
     1561 tagcttggag gaggttcacc catggggttg cagcgctacg cacccgattg aagcgctggc
     1621 aacttgccat ggggcgatac ttctccctcg tccgccagga caacatgctg gccggagtcc
     1681 gcgttatctt cgcccctcgc agcttgcagc ggcatgcggg gcgtagcctt gccacaaggc
     1741 tggcctgccc ctaccgtcca aacctgcgca ggcaggtttg gatctgcggc tttcgcccta
     1801 cccagccgcg ccgccagccg cccactcggt cgcgttcaat tgagggatct aggatgatcg
     1861 aagctatcgc tgccctggtg ctgggggcct tgttgggtgg cgctgtcatg gcctggcgct
     1921 gccagcagct caagcaggcg atgcgcgcca tggtgcctgg tcaagtgctt caatcggtgc
     1981 aagttcaact gaatgaggca ttggcccagt tacaggccca aagcgatcag tatcaagggc
     2041 gtgaacagga aattctcggc gaagctactg agctgtctcg aaaccttgac gagcaccagc
     2101 aattgctgac gcaaattcag gccaattttt cagcggtcaa cggtcaggtg ctggttgaat
     2161 gtgaagcgtt tggcagtggt gtcggcaact tgctgggcct ggtcaagacc tttgagcgct
     2221 ggcacgccga catgaacaac ctgatcattc acaaccggga aatgcatggc aagaacgatg
     2281 atttcagtgc catcgtgcga caaatggtga ttgtgacgct caatgcctca atcgaagccg
     2341 cgcgtgctgg cgagcaggga cgtggcttct cagtggtggc cgatgagatg agggcactgg
     2401 cggggcgcgc cgagaagctg tccgcagact accgcaagaa tctgtatcaa aacgatttga
     2461 tcacggtcaa tacgtttcag gacttgcagg ccggcggcaa gatgatcatt ggttcattgg
     2521 tcggtctcga acagaccttc aggaatatca gggagcgtct gagcgctgaa ccatcgccat
     2581 gatcagtaac aacgcttgtg agagctttga tcacctgctg ctgcaaggca tcaggagttg
     2641 tctggtcaac caggacagcg acagttgtga catcagtgtt caggatgatc tgacgggctc
     2701 acacgagtct catctggctg ttctgacgat ttcttcgtac ctgtttcgat tgatggtgtt
     2761 gattcatttt tcagcagacg agccaacccg cgagcacctg gccaagttga gtaatgccag
     2821 cgtcaacgat ttgaacgaac aggctttttt ggatgcaatt tgtgagcgcg caaatatgtg
     2881 ctgtggttcg ctgagccgag acttgtccca gactttcccg catattggca tgtcaacccc
     2941 gaatatcgtt gaccggcaat gcgcttcaca tctgcattcg ctcaatcctg gtcacaagcg
     3001 ccacttcaag gtaaccgtca acagcacggc gcagttccat gtcacgctgt gtgtgagcag
     3061 ctacgacaag ctggatttcg tcgtcgacaa gtgcgccgcc gaggttcatg gcggcgaact
     3121 ggaaatgttt tgacaggcac ccaactatcc ggagaacacc attccatgaa cgaagaaatc
     3181 aaagttgtca gcaaatttct ggtgctcgac gatgaccata gttgccatca acgaatcaag
     3241 agcttttgcg acgacaacag cctggtgccg ctgaaagtgc aggccgacaa tgtgatgagt
     3301 gtgttgaagt ccaaggttga cttgggaggc atcttgctgt ccgagaattt tggggccgat
     3361 gcgggtgaag gcatcaggtt ggggcatttg attcatgccg ttcgcccgga actgccgttg
     3421 ttcttacggc gggagaattg cgaaaacctt gatgatttgt cgccaccgga tcaacggata
     3481 ttcgccgcag cttatacgat cagtaacatg gcccatcttc gggcggcact ggacgaatcg
     3541 attttcagct ttgtctatcc caatgttttg gtgcggggca tttcggaatt gacaagcgct
     3601 gccctggaga gccagtttat cgacatgaaa gccgaagttg aagcacccta tattgtgcgc
     3661 gacaagttga ttttcggtga aattttcacg ctcataccga tagaaaacaa ctggtgtcgg
     3721 ggttacatga tgttgcagtc agaagagcgg tcgctgctgg aactggttaa aggtgaccgc
     3781 acacatgtca gcgccgacaa ggccgacgat ttccgcagtc tcaacagcgt gctgggcgag
     3841 atcaccaacc tgatctgggg aggcttcaaa agccggtact caggtgccgc cagcgccgac
     3901 cgcaatttgt cccaggtgcc gatcatcatc aatcatttgc atcgctacat ctcatttggc
     3961 tccgacaacc cgcaactgtg ttttcgctac accttgactg accgggcgca cgttcaggca
     4021 cgttcactgg tgatcaacca gaagtttgtc ttcaacctga actggagtcc tgatgatttt
     4081 tcagagaacg tgacatctgt ggaagacttg gtcagcgcag gtgaactcga tttgttttga
     4141 tcttggagta acacatggca caaatattgg tggtagatga ttcgagcacg gttcgcaatg
     4201 aagtaggaga ctttctgacc aaaagcggca tgaccgtgac tttggcggtt gacggacgtg
     4261 atggtctggc aaaactcaag tctgattcat ccatcaagtt ggtcgtctgc gatgtcaata
     4321 tgcccaatat ggacggactg acgatggcgg aaaaggtacg tagcgaactg cacaatcaga
     4381 gcgtcaacat catcatgttg acaaccgaaa attctccagc catgaaagag cggggcaagg
     4441 cggctggcat caagggctgg atcgtcaagc cgttcaaagg tgatgccgta ttggcaacct
     4501 tcaagaaact tgttggtgca taggaggagc caagttacca gcgtatgacg aaaaactcaa
     4561 cgcgtttagc tgccgtttga gcatttgagt gagaacaaat ttacc
//
LOCUS       DAOSLL010000386         4601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16594_length_4601_cov_3.076991, whole genome shotgun sequence.
ACCESSION   DAOSLL010000386 DAOSLL010000000
VERSION     DAOSLL010000386.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16594_length_4601_cov_3.076991"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="PLB25_15270"
     CDS             complement(<1..910)
                     /locus_tag="PLB25_15270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE42969.1"
                     /translation="MKISLVMPSHRCNNAAISRIMELTTLDPAKYELIVRDNSESLYK
                     RELITHFQTSTLKYVPVSNCAPMSNASEAFKLATGEFTFFLGDDDRLPINGIEAIYKT
                     VEENNADSSFGIATGTYLVESTSGSGLFAYPLLDQHDPNHRIASYLNEANAMNFIYYS
                     ATRTSTARDIFKFVENLPYQFSYVDLLMTLLRLTQGRALKTEQIIYHYDYSQWNSLKD
                     SHNKDGSFYVAAGLPVEYNHLHYLFCGLEGALILDSNWVLERTGSDMSSASLVWFNHM
                     FGRFVTQERVFAYENTKANQAILGYKK"
     gene            complement(1645..2163)
                     /locus_tag="PLB25_15275"
     CDS             complement(1645..2163)
                     /locus_tag="PLB25_15275"
                     /inference="COORDINATES: protein motif:HMM:NF022593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 family protein"
                     /protein_id="HOE42970.1"
                     /translation="MGSMRGDSFRSNCQSAFERSMKQVDPYEELNVNIYGVLKPPLLL
                     WLIMLIETWHFWSSIFIAFSGETWLFTKGFGWMNFAAEAPALLVLLALSARMPKMPKF
                     VPMIWHRGRELLTLAAVGNLGIIVFGAIQVDFWRLSTEWSTLLAVFLHVWVLLRVWVS
                     PIIQKVFSEFPA"
     gene            2352..2516
                     /locus_tag="PLB25_15280"
     CDS             2352..2516
                     /locus_tag="PLB25_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42971.1"
                     /translation="MTYLHDTDFKPIFTRNDLLRELLEVFAPAGLTELPGYSTLRPEN
                     GCKTTSIGAP"
     gene            complement(2771..>4601)
                     /locus_tag="PLB25_15285"
     CDS             complement(2771..>4601)
                     /locus_tag="PLB25_15285"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE42972.1"
                     /translation="STRSGVDKWIKVRAIPKQRGDGSILWNGILTDVTHSKRIEQAAR
                     AASLAKSEFLANMSHEIRTPMNGVIGMVDILQHSDLLPQQRRMLDTIANSAQLLLHIL
                     NDILDYSKIEAGKLAVERLATPLEPVAASVLQLMQGSASAKGITLSMSIDPGLPAAIY
                     SDPTRLRQVLLNLIGNAIKFTPETSGHKPGVLLRIEAGALPDGQPGVLLRVIDQGMGM
                     PADLVGRLFQPFTQADASTAREFGGTGLGLSISQRLVALMGGQLTAQSTPGEGSVFTV
                     ALPLQGAPVEPMPTFVPERRLLLRTSAPSVAQAAASGHLILLAEDNETNSEVLREQLR
                     LLGYASEVAVDGVAALALWRSGRFGLLLTDCHMPCMDGFELTTAIRQQEAAGSRLPII
                     AVTANAMRGEALRCLAHGMDDYLSKPLRLQELGPVLAKWLPLPPDPQAAAQWVALLQG
                     QTGTPGPGQPEVGCCDADTNANTDPAGLAIWDARTLSRLVGNNLGLHRRLLEKFLVKA
                     TDQVRVIELAAAAGDLKPVAELAHSLKSASRSMGALALGELCRQIETAGLANDVSACH
                     TLVAGLGNSFTLAQASIVAHLAKTAAPAAVPLALPSGASGQPG"
BASE COUNT         1054 a         1301 c         1225 g         1021 t
ORIGIN      
        1 gctttttgta gccaagaatc gcttggtttg ctttggtatt ttcgtacgcg aacacccttt
       61 cttgcgttac gaatcttcca aacatgtgat tgaaccacac caaggatgct gacgacatgt
      121 cagacccagt gcgctctaga acccaattgg agtcaagaat caacgcccct tcaagaccgc
      181 aaaacaagta gtgcagatgg ttgtattcga cgggcaaccc agccgcaacg tagaaacttc
      241 catccttatt gtgcgagtct tttaagctat tccactgact atagtcgtag tggtaaatga
      301 tttgttccgt tttgagcgct cttccttggg ttaggcgaag aagcgtcatc agcaggtcga
      361 catacgaaaa ctgataaggg aggttttcga caaatttgaa gatatccctt gcagtcgatg
      421 ttcttgttgc tgagtagtag atgaagttca tggcgtttgc ctcatttaaa taggaggcaa
      481 tgcgatgatt tgggtcatgt tggtcaagga gggggtatgc aaacagacca cttccagagg
      541 tggactccac taaataagtg ccggtggcta taccaaacga cgaatcggca ttgttttcct
      601 cgacggtctt gtatattgct tcgataccat tgatcggcaa tcggtcatca tcaccaagaa
      661 agaaagtgaa ttctccggtt gcaagtttga atgcctctga agcgtttgac attggagcgc
      721 aattcgacac tggaacatac ttcagcgtgc tcgtctgaaa atgtgttatc aactcacgtt
      781 tgtataagct ctcagaattg tctcgaacaa tcagctcata cttagccgga tcaagcgtcg
      841 tgagctccat aatccgggaa atggccgcgt tgttgcagcg gtggcttggc attacaagag
      901 atattttcat gaccatagac actgtaaatc aagatttgcc ggatggcttt cgcaaacaag
      961 tgctccgagc tcaaggatta agacgtctca ggtagccgtc gggggccact gtaataagta
     1021 atttgtttgg ctctatgccg ttttgtgtgg gacttgacaa tccgagccaa atggggtgaa
     1081 ggtgcctttt gtacaaaact gagttcggca cagaaagcag tgatcactat gttgatagtt
     1141 ggagcctccg atgtcagaca aggcttttcg cctcaacatg ttgattcgac aagccttttt
     1201 caatggtctg ccacgttttg actgcgtaaa ggccaagccc ttcgggtgga cttcgttcag
     1261 ccttgacaca gtcaaatcgc agcggcgaaa cgccatacac cacccgcgtt gcttttgcaa
     1321 cttccacacg aaacgacgaa gggccttcag gtgggactct tcactaatcc ggttgcctaa
     1381 tcccccggca agtattactg ctttcaaaat tgaactccag cgtaaatcag caagcaaaga
     1441 ctaaagaggc aggcatccaa agcagaatta tgatgcacca ggcctccaaa tgagattcgc
     1501 gtttgggtaa tctgacactt gcaaatgtcg ccttgatctt ttgtcgcaaa gacacgctcc
     1561 caagacaatc tgtggcggtc tttgacaatt tcgcactttt cgggtgtgtg gcatctttaa
     1621 tcaacggaca actagggtaa cttttcaagc tgggaactcg gaaaacacct tctggattat
     1681 gggagacacc cagacccgca gcaacaccca aacatgcaga aaaaccgcca acagggttga
     1741 ccattctgtg ctcaatcgcc agaagtccac ctgaatcgcc ccaaaaacaa tgatgccaag
     1801 attcccaaca gcggctagcg ttaacaattc cctgccccgg tgccagatca ttggcacaaa
     1861 cttcggcatt ttgggcatgc gggcagataa cgctaacagc accagcagcg caggcgcttc
     1921 ggcggcaaag ttcatccagc caaacccctt ggtaaacagc caggtctcgc ccgagaaggc
     1981 gataaaaatc gatgaccaaa aatgccatgt ttcaatcagc atgatcagcc acagcagcaa
     2041 cggtggtttg agcacaccgt agatattgac gttcagttcc tcgtatggat caacttgttt
     2101 catacttctt tcaaatgcag actggcagtt tgagcgaaag gaatcacccc gcatcgaacc
     2161 cattcaaatt cttgcgaatg catgattgaa atgggagtgt tcagggtcat ccgttcagtc
     2221 ggggtcaggg tcaaattgct tcctacaggc gcagttagct atatttaaaa tagcttgcag
     2281 ggcaatacag gtaagcgcca gagccaaaaa cacgcatcat ttttgaacgg cacattgatt
     2341 caatcgaatc catgacatat ctgcatgata ccgacttcaa acccatcttc acgcgcaatg
     2401 acctgctgcg cgagctgctc gaagtgtttg ccccggcggg cttgaccgaa ctgccgggct
     2461 acagcaccct gcgcccggaa aacggctgca agaccacatc aatcggtgcg ccctaatttt
     2521 tgaccaggcc agtcaaattc aaaaattcaa acgggccagt tgacgtgatg gcgcagattg
     2581 ggttattggc accaagtctg ccaaatccgc acgtcgccag ccattgagac ccagctctca
     2641 atgctgcaca cattgagctg gcatcactca gcccggtcga aaaagtccaa cccctttttg
     2701 tctttttctg atcccacagg caccccgagg ctggcgccag cgctgcagat ggcgctgacc
     2761 cgcactgaag tcatcctggc tggccagaag cgccagatgg caacgccagc ggcaccgcgg
     2821 ctggagccgc cgttttcgcc agatgggcga cgatgcttgc ctgcgccagc gtgaagctgt
     2881 tgcccaggcc agctaccagc gtatggcagg cgctgacatc attggccagg ccggctgttt
     2941 cgatctgccg acacagctcg cctagcgcca gcgcacccat tgaccgactg gctgatttga
     3001 ggctgtgcgc cagctcggca acaggcttga gatcacctgc cgcagcagca agctcgatca
     3061 ccctgacctg atctgtcgct ttgaccagga acttctccag caagcgccgg tgcaggccga
     3121 ggttgttgcc aaccaatcgg ctcagggtac gcgcatccca tatagccaac ccggccgggt
     3181 cggtatttgc gttcgtatcc gcatcacagc accccacctc gggttgacca gggcctggtg
     3241 tcccggtttg cccctgcaac agcgccaccc actgcgctgc tgcctgcggg tcgggcggca
     3301 gcggtagcca cttggccagt accggcccga gctcttgcag gcgcagcggc ttgctcaggt
     3361 agtcgtccat gccgtgcgcc aggcagcgca aagcctcacc ccgcatggcg ttggcggtga
     3421 ctgcgatgat cggcagtcgg ctgccggctg cctcttgctg gcgaatggcg gtggttagct
     3481 caaaaccatc catgcacggc atgtggcaat cggtcagcag cagcccaaag cggccgctgc
     3541 gccagagcgc cagcgcggcc acaccatcca ctgccacttc gctggcatag cccagcaggc
     3601 gcagttgctc gcgcagcacc tcgctgttgg tttcgttgtc ttcggccagc aaaatcaggt
     3661 ggccactggc ggcagcttgc gccacgctgg gggcagacgt tctgagcaac aaccggcgct
     3721 ccgggacgaa agtcggcatc ggttcaactg gtgcgccctg caagggcagc gccacggtaa
     3781 acacagaacc ctcgccaggc gtgctttgcg ccgtgagttg cccacccatc agcgccacca
     3841 gccgctggct gatcgacaaa cccagcccgg tgccgccaaa ttcgcgtgcg gtgctggcat
     3901 cagcctgggt aaacggctgg aacagccgac ccaccaggtc tgctggcatg cccatgcctt
     3961 ggtcgatgac acgcagcagc acgcccggct ggccatctgg cagcgcgccc gcttcgatgc
     4021 gcagcagcac gccaggcttg tgcccagacg tttccggggt gaacttgatg gcattgccga
     4081 tcaggttgag cagcacctgg cgcagtcgcg tcgggtccga gtagattgcc gctggcaacc
     4141 cggggtcgat tgacatcgac agtgttatcc ccttggcgct ggcgctgccc tgcatcagtt
     4201 gcagcacact ggcggccacc ggctcaagcg gcgtggccag gcgctcaacc gccagcttgc
     4261 cagcctcgat cttggagtag tccagaatgt cgttgaggat gtgcagcagc aattgcgccg
     4321 agttggcaat ggtgtcgagc atgcggcgtt gttggggcaa caagtcactg tgctgcaaga
     4381 tatcgaccat gccgatcaca ccattcatgg gggtgcggat ttcgtgactc atgttggcca
     4441 aaaactccga cttggccagg ctggccgccc gtgccgcctg ctcaatgcgt ttgctgtggg
     4501 tgacatcggt caagatgccg ttccacagga tgctgccatc gccgcgttgt ttcggtatgg
     4561 cgcgcacctt aatccatttg tcgacgccgc tgcgggtgct g
//
LOCUS       DAOSLL010000387         4600 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16598_length_4600_cov_4.578438, whole genome shotgun sequence.
ACCESSION   DAOSLL010000387 DAOSLL010000000
VERSION     DAOSLL010000387.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16598_length_4600_cov_4.578438"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="PLB25_15290"
     CDS             <1..1175
                     /locus_tag="PLB25_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding protein"
                     /protein_id="HOE42973.1"
                     /translation="FLEGAAIPAGAEDAYFVPAVSDQQASVYEHAARAIDRSLKVGVH
                     GLPLMGSGDWNDGMNQVGIEGRGESVWLAWFLCRLVADFAPLAHQRGDHLRADRWTQA
                     ASGWQAALQGQAWDGSWFKRAFFDDGQPLGASANPEGRIDLIAQAWAVLSNAAPLEQQ
                     AQAMAAAEALLVDVEVGLIRLLDPPLVRSLPSAGYIQAYPPGVRENGGQYTHAGVWAL
                     MAQAQLAQTTVPDSATDASGAGDLVYRYLTYLSPAHRSAHPTRGPLYGLEPYVVAGDV
                     YSQPPYAGRGGWSWYTGAAAWMHRAVLESIFGLSQRARSLSFKPCLPGHWPQAELTLV
                     RDARRLHFILMRATPNAALAAHASQHARLLMPGQWLAWTDLPMQTCFVVPLLRDAL"
     gene            1705..2019
                     /locus_tag="PLB25_15295"
     CDS             1705..2019
                     /locus_tag="PLB25_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HOE42974.1"
                     /translation="MFIRATLAMAAAALIVISATGCAVSRGQETMGAYVDDAGITTLV
                     KTRMAESKLVSASSISVETLNGTVMLSGFAKSSTEKNEAERIARGVNNVKVVKNEIAV
                     RP"
     gene            complement(2200..2643)
                     /locus_tag="PLB25_15300"
     CDS             complement(2200..2643)
                     /locus_tag="PLB25_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YXWGXW repeat-containing protein"
                     /protein_id="HOE42975.1"
                     /translation="MKIKRVAQSLLLSVTLTTSMAVLAQVQLRIHIGPPAPIDEYRPT
                     LAPGHVWAAGYWAWSHDRHVWVRGRSMLGRPGYHWEPDHWQQRGETYYRQPGNWTRNP
                     QMQQPAAQPARQAAQKQHGQKPKNKPRHGAPKHNPWEDKKDKQRQ"
     gene            2775..4169
                     /locus_tag="PLB25_15305"
     CDS             2775..4169
                     /locus_tag="PLB25_15305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006413584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOE42976.1"
                     /translation="MPLFISPQRHHLAVLALCLFNAGGCASLPDTQAILARHTHQQAM
                     FESALGPVSAKRQASVLAGLKRGTGDIDILDKQIALEQAIVGSPLVLGNKTTLLQDGP
                     ATYAAMFAAMAQAQDHINLESYIIEDDAVGHQFVELLLAQQRRGVQVNLIYDSVGGLN
                     TPKLFFEPLLQAGAAVLEFNPINPLEARGEWLLNNRDHRKLLVVDGRTAFIGGINISS
                     VYSSGSILRRPKKTTGNAAWRDTDLQLQGPVVAEFQKLFMATWHKQHGPALAAKNYFP
                     ALGVAGQDIVRAIGSTPDDPYSLIYLTLMSAIGNAERQVFLTNAYFVPDPQLLQALTD
                     AAARGVDVRLILPSYSDSGLVFHAGRAHYSRLLDGGVHIFEREGALLHSKTAVIDGIW
                     STVGSTNLDWRSFMDNDEVNAVILGRDFAAQMLAVFARDQAASQAIEPNTWQQRPLQF
                     KLKEWLAQLWERWL"
BASE COUNT          832 a         1430 c         1401 g          937 t
ORIGIN      
        1 cgtttctgga aggtgccgcg attcctgcgg gtgccgaaga tgcctacttt gtccctgccg
       61 tgagcgacca gcaagcctcg gtgtatgagc atgcggcacg ggccatcgac cgcagcctga
      121 aagtcggggt gcatggcttg ccgctgatgg gcagcggcga ctggaatgac ggcatgaacc
      181 aggtcggcat tgaggggcgt ggcgagtcgg tctggctggc ctggtttttg tgccgcctgg
      241 tagcagactt tgcgcccctg gcgcaccagc gcggtgacca tctgcgcgcc gaccgctgga
      301 cgcaggcggc cagcggctgg caagcagcgc tgcaaggcca ggcctgggat ggctcatggt
      361 tcaagcgggc gttttttgac gatggccagc cacttggtgc cagcgccaat cctgagggcc
      421 gcattgacct gattgcccag gcctgggcgg tgctgtccaa tgccgcaccg ctggagcaac
      481 aagcgcaggc gatggccgcc gccgaggcgc ttttggtgga tgtcgaagtt gggctgatcc
      541 gtttgcttga cccgccattg gtgcggtccc tgcccagcgc tggctatatc caggcctacc
      601 cgccaggggt gcgcgaaaac ggtggccaat acacccacgc gggtgtctgg gcgttgatgg
      661 cgcaagccca actggcgcaa accactgtgc cggacagcgc caccgacgct tctggcgctg
      721 gcgacctggt ttaccgctac ctcacctatt tgagcccggc ccaccggtct gcccacccca
      781 cccgcggccc gctgtatggg ctggagccgt atgtggtggc gggcgacgtg tacagtcagc
      841 cgccctatgc ggggcggggc ggctggagct ggtacaccgg tgcggcggcc tggatgcacc
      901 gggccgtgct ggaatcaatt tttggcctga gccaacgcgc ccggagcttg tcattcaagc
      961 cctgtctgcc cggccattgg ccgcaggccg agttgacgct ggtgcgagat gcgcgccgcc
     1021 tgcacttcat cctgatgcgt gccacaccaa atgcagcgct ggctgcccat gcatcgcaac
     1081 atgcccggct gctgatgccc ggccagtggc tggcctggac cgatttgcca atgcaaacct
     1141 gctttgtcgt gcccctgttg cgagacgccc tgtaggagcg ctaccctggc cgcgaactgg
     1201 ttttcgcggc cggggcgccg ctcctgcacc gcttcatctc tttaggcgct atgcgcgtca
     1261 aacttaccaa catgttgtcg gtctttgccg actgtctcgg gcagcaggca tcccgttttg
     1321 aacctgttgt gggccatggc tagtcgccaa aggcgaacct cattttccca gcttgtctgg
     1381 tgaaaatgcc tggtcactaa caagtcaagt ctttgcatat catgcgaaga attcaaacat
     1441 tccaagtcat tcgtcgcgag ggcgcgactc ctacaaattc aaattggtgc cgactgtttg
     1501 tgtaggagtc gcgccctcgc gacgaaggcc gtccaattcc ggcttgtccg gcttagggtg
     1561 agcaaacggc gacggcggtg ggtgcgccag cgcaccgaca gaatgccagg caagttttaa
     1621 agtcaccaca caaacttggg cttgtccggg ttttttgccc atcggtggtg gggctttcac
     1681 catcaaacac attggagaat cattatgttt attcgagcca ccctcgccat ggccgcggcc
     1741 gccctgattg tgatttctgc caccggttgc gccgttagcc gtggtcagga aaccatgggt
     1801 gcctatgtgg acgatgccgg catcaccacg ctggtcaaga cccgcatggc cgagagcaag
     1861 ctggtcagtg cctcgtcgat cagtgttgaa accctcaacg gcaccgtgat gctgtcgggt
     1921 tttgccaaaa gcagcaccga gaagaatgaa gctgaacgca ttgcacgcgg cgtgaacaac
     1981 gtcaaggtgg tgaaaaacga aattgccgtg cgcccctaag cggtcagtca gcctttgtac
     2041 agcaccaggt gctggtgcct gtaccgttaa cctggcagac tcttgccacc ccggatccgc
     2101 ctattgggtc acggggcttt ttgccttgtg cactgagtca aaaatgccga cgggctgatc
     2161 tgccaccagc cagttcctgc cagccaggct ggctgttgtt cactgccgct gcttgtcctt
     2221 tttgtcttcc caggggttgt gtttgggagc accgtggcgg ggcttgtttt tcggcttttg
     2281 accgtgctgc ttctgcgctg cctgccgtgc tggctgcgct gcgggctgct gcatttgtgg
     2341 gttacgcgtc caattgcccg gctggcggta ataggtttcg ccgcgctgct gccagtggtc
     2401 tggttcccag tgatagccgg ggcgacccag catggagcgc ccgcgcaccc agacgtggcg
     2461 gtcgtggctc caggcccagt agccggcggc ccatacatgg ccgggggcca gtgtcggccg
     2521 gtactcatca attggcgctg gcgggccgat atggatgcgc aattgcacct gtgccaggac
     2581 agccatgctg gtggtcaggg tgacggacag caacaggctt tgagcgacgc gcttgatttt
     2641 catgaaaaaa aaccttgaga ttgggttaaa tcgtcatcgt cgcggcaatc cagagcggcg
     2701 tcggtatgcc agcgcacaaa aaacagcggc ctgccctgcg taaacttgga caaatcccac
     2761 ttcgggtcaa caccatgccg ctcttcatca gcccgcagcg ccaccacctg gcggtgcttg
     2821 ccttgtgcct cttcaatgcc ggtgggtgtg ccagcttgcc cgatacccag gccattctgg
     2881 cgcgccacac gcaccagcag gcaatgtttg aatccgcgct tgggccagtc tctgcaaagc
     2941 gccaggccag tgtgctggcc gggctcaaac gtggcaccgg cgacatcgat atcctggaca
     3001 agcaaatcgc gctggaacaa gccattgtgg gcagcccttt ggttcttggc aacaaaacca
     3061 ccttgctgca agacggtcct gccacttatg ccgccatgtt tgccgccatg gcccaggcgc
     3121 aagaccatat caacctggag tcctacatca tcgaagacga tgccgtgggc catcaatttg
     3181 tcgagttgct gctggcgcag cagcgccggg gcgttcaggt caacctgatc tatgacagcg
     3241 tgggcggctt gaacaccccg aagctgtttt ttgaaccctt gctacaggcc ggggccgcgg
     3301 tgctggaatt caacccgatc aacccgctgg aagcccgcgg cgaatggctg ctcaacaacc
     3361 gcgatcaccg caaactgctg gtggttgacg ggcgcacagc cttcataggc ggcatcaaca
     3421 tcagcagcgt ctattcctct ggctcgatcc tgcgacgccc caaaaaaacc accggcaacg
     3481 ccgcctggcg cgacaccgac ctgcagttgc aaggcccggt ggtggccgag tttcaaaagc
     3541 tgttcatggc aacctggcac aagcagcatg gcccggcgct ggccgccaaa aactactttc
     3601 cagcactcgg cgtggcaggc caggacatcg tgcgggccat cggcagcacg cccgatgacc
     3661 cctatagcct gatttacctg acgctgatgt cggccattgg caatgccgag cgccaggtgt
     3721 tcctgaccaa cgcctacttt gtgcccgacc cgcaactgct gcaggccctc accgacgcgg
     3781 ctgcgcgtgg tgtcgatgtg cgcctgattc tgccgagcta cagcgactcc ggcctggtgt
     3841 ttcatgccgg gcgggcgcac tacagcaggc tgctcgatgg cggcgtgcat atttttgagc
     3901 gcgaaggcgc gctgctgcat tccaagactg cggtgattga cggcatctgg tccaccgtag
     3961 gctccaccaa cctcgactgg cgcagcttca tggacaacga cgaggtcaac gcggtcatcc
     4021 tgggccgcga ctttgcagcg caaatgctgg ctgtgtttgc ccgtgaccag gccgcctcgc
     4081 aagccatcga gccaaacacc tggcaacagc gcccgctgca gttcaagctg aaggaatggc
     4141 tggcgcagtt gtgggagcgc tggctctgag tcagcccgcg cgggcctggc acctgtagga
     4201 gcggcaccct gtaggagctg cgcccgctca gcgaaagcgc tggtgttaat ttggctttta
     4261 tgcccaacac ctgcaggtgc tgcacccacg cagcgaaggc ttccaccgcg ctaacgtagc
     4321 gccttcaagg gcgcactagg agctgcgccc tcgcagcgaa gttttcggct tccttgttgg
     4381 tgcaggaaag actgaatgtg tcactggctt taatgccgcc tgggttcgct atcgcaccga
     4441 caagctcgcc acaaggcgac aaagtcgggg ctggttcctc acatccaaac cggagatttt
     4501 tttatgaaca accccctggg cctgcgcctc ctgatcacca ccgtcgccgg ggtcatgacc
     4561 ctctcgatgg tcgcttgcaa caagcccgcc gacaccagcg
//
LOCUS       DAOSLL010000388         4597 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16609_length_4597_cov_5.066711, whole genome shotgun sequence.
ACCESSION   DAOSLL010000388 DAOSLL010000000
VERSION     DAOSLL010000388.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4597
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16609_length_4597_cov_5.066711"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="PLB25_15310"
     CDS             complement(<1..769)
                     /locus_tag="PLB25_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HOE42977.1"
                     /translation="MQKIISQIAQELRVHESQVACAVELLDGGATVPFIARYRKEATG
                     GLDDIALRELDTRLAYLRELRDRLAAVIKSIEEQGKMTPALLAALVAAPTKQELEDLY
                     LPYKQKRRTKGQIAREAGLEPLADRLFADPTQDPAEQAQAFIKPEKNEAGDDFTTLQA
                     VLDGVRDILSERWAEDAALLQDLRAWLWAEGLFKSRLIDGKNENDPEVAKFRDYFDYD
                     EPIGRVPSHRALAVFRGRSLEILEARLVLPEANPALAA"
     gene            1500..3623
                     /locus_tag="PLB25_15315"
     CDS             1500..3623
                     /locus_tag="PLB25_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOE42978.1"
                     /translation="MNEPQPEDIRNRLLVARLPAMPQILLKLLQLCQADEVGMTELAK
                     LIANDAAMTNRVLQVANSAAYQRSGRKVGLMQALSVLGADQVKSLIVSGSVLQAFSGF
                     PHTASLDLRGFWKHALTAAVVARGIAKQMDYAQSEEAYLAGLLHDVGRLALLAAAPAV
                     YHINFQTPDNENLCAIEQRTLHISHAEAGAWLIERWQMDSFIADSVLYHHEDSARLET
                     AHPLIRIVHLAHLLSDQPPDQPLVADIGAICALTDEQVQAIYQAAGAQVKLAAEQLGI
                     DLAGVDKLAVSVAVASPLPAASPVQQRLTEEIRNRALIAELGQLFARQRDDAHLLESV
                     RQNVRVLFDLDDTVVFLMNGGGQALIGASFGGQRQRLAELAISLTGSGGLAESVLQRS
                     LVFVSQERGLLSLAEDQLLRVFGTQCLICLPIASSTRSLGLLVGSVESWRIAELKRQE
                     KFLLAFGAQAAQALENSARERGEIDRRIARLRDEHLLDSRKVAHEVNNPLAIIKNYLG
                     VLDDKLARDEPVAAELSILHGEVDRVGSIIKQFAGITPAPPDSVVEINQVINDIVRLF
                     RGSRFLPASVQIIAQLPGQSCEITGVASVLKQIFANLIKNAVEAMPKGGRIEVINRGA
                     VRRDRIDYFAMLVRDTGPGLPDAVMARLFSPVRSSKPGENRGLGLSIVHDLVQKLHGQ
                     INWNSSNLGTAFEILLPRRPSKSAN"
     gene            3624..>4597
                     /locus_tag="PLB25_15320"
     CDS             3624..>4597
                     /locus_tag="PLB25_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE42979.1"
                     /translation="MKLNDPTRRGCPLTSDSLIPPKAVPTPAQLLLVDDEPLMLASLC
                     ELLKHRDFVLTTADCGAAAIDLLRHTVFDLVILDLLLPDISGQQVMEFMNAQGIDTNV
                     IVISGASGIDAAIGALRAGAYDYLRKPYPREELLKAVQNALQQHRLAAENRQIAGQLA
                     SSERIYRYLVDSSPDIIFTLNHQGIITFVNDRVHPLLGFNREDLIGQHYAELVHDQDI
                     ERADYVFGDSQRDHCWSRNIELRLKSHDVDDEGRSFRIDLMTISLDNGQHCVTTGSTP
                     RDKLGIYGVARDITESKRADELITYQAYHDILTDLPNRVLFKDRLSLS"
BASE COUNT          907 a         1400 c         1357 g          933 t
ORIGIN      
        1 ccgccgccag cgccgggttt gcctcaggca gtaccagccg ggcttccaga atctccagac
       61 tgcgcccgcg aaacaccgct agcgcccggt gcgacggcac ccggccaatc ggctcgtcat
      121 agtcaaaata gtccctgaac ttggccactt cggggtcgtt ttcgttcttg ccgtcgatca
      181 gtctgctctt gaacaggccc tctgcccaca accaggcgcg caggtcttgc agcagcgccg
      241 cgtcttcggc ccagcgttcg ctcagaatgt cgcgaacacc gtctagcacc gcctgcagcg
      301 tggtgaagtc gtcacctgct tcgttcttct cgggcttgat aaaggcttgc gcctgctctg
      361 cgggatcttg cgtcgggtca gcaaacagcc ggtcggccag cggctccagg cctgcctcac
      421 gggcgatttg ccctttggtc cggcgctttt gcttgtaggg caggtacagg tcttcgagtt
      481 cctgcttggt cggcgcagcc accagtgccg ccagcaaggc cggggtcatc ttgccctgct
      541 cttcgatgct cttgatgacg gccgccaggc ggtcgcgcag ttcacgcagg taagccagac
      601 gggtgtcaag ctcgcgcagc gcgatgtcgt cgaggccgcc ggtggcctct ttgcgatagc
      661 gggcaataaa gggcacggtg gcaccaccgt cgagcagttc cacggcgcag gccacctgac
      721 tctcgtgaac ccgcaattct tgcgctatct ggctgatgat tttttgcata acccaacgaa
      781 aagccagcac gaagctggct tgaaaaaatg tcgatcacgc aagtgtgcca cacgcatcgg
      841 catcggcatc ggcatcggca tcggcatcag catcggcctg acctggtaac cgcgtcaact
      901 gcacggatca aagcgcggcg gctgctggtt cagtcagcgt ttcatcagca ggtcttcctt
      961 gaacccggat tcctcgcact caaagtggcc ttgaccgcag cctgcccacc cgtgcaacac
     1021 cacggccaac tgaggcggct tggcgggcct tcgggctgac cctggcctgg ttgttgagct
     1081 cgatcaggtc agtgtgatca gcatcgatca gaccattgcc aacgctgaat tgttcacgcc
     1141 cttggactga cattgggcgc gcggctcatc aagaccacct tgttgctttg accccaacgg
     1201 caatttatcg gcgagtgttg gttgggaccg acactggcca atgccagatt cgaatgacca
     1261 gccacccgaa cagcttgccc aggcgatctg cacagctcta ttttttatag cacaaaaggc
     1321 ttgtgcaact tggccttcaa gtcagatcca tccgatattt ttggtgctga caggacaccc
     1381 aaaaaaaacc aaatctgcac ctttgctgtt atattgaaaa ggttagaaga tgtgcttggc
     1441 gcaccaattc acacaaagag aactgtttag cccgtttgcc attgtggccc gagtccttca
     1501 tgaacgaacc tcaacctgaa gacatccgca accgactgct ggtggcacgg ctaccggcca
     1561 tgccgcagat tttgcttaag ctgctgcagt tgtgccaggc cgatgaggtc ggcatgaccg
     1621 agcttgccaa actgattgcc aatgatgccg ccatgacgaa ccgcgtgttg caggtggcca
     1681 acagtgccgc ctaccagcgc agcggccgca aggtgggcct gatgcaggcc ctcagcgtgc
     1741 tcggggccga ccaggtcaaa agtctgatcg tcagcggatc ggtgctgcag gccttcagtg
     1801 gtttcccgca taccgccagc cttgacctgc gcggcttctg gaagcacgcg ctgaccgccg
     1861 ccgtagtggc acgcggcatt gccaaacaga tggactatgc acagtccgaa gaggcctacc
     1921 tggcgggttt gctgcacgat gtcggccgac tcgccctgct ggccgccgca cctgcggtct
     1981 accacatcaa ctttcagaca ccggacaacg aaaacctgtg tgccatcgag cagcgcaccc
     2041 tgcatatctc gcatgccgaa gccggtgcct ggctgatcga gcgctggcag atggactcat
     2101 tcattgccga tagtgtgctt taccaccatg aggattccgc ccgccttgag accgctcacc
     2161 cgttgatccg catcgtgcac ctggcgcacc tgctcagcga ccagccaccc gaccagccgc
     2221 tggtcgccga cattggcgcg atttgcgcgc tgacagacga acaggtgcaa gcgatctacc
     2281 aggctgccgg ggcgcaggtc aagttggcag cagagcagtt gggcattgat ctggctggcg
     2341 ttgacaaatt ggccgtgtct gtggccgtgg catcgccctt gcctgcggcc agtccggtgc
     2401 agcaacgcct caccgaggag attcgcaacc gggccctgat cgccgagctt ggccagttgt
     2461 ttgcccgcca gcgcgatgat gcccatttgc tggagagtgt gcggcagaac gtccgcgttt
     2521 tgtttgacct tgacgacacg gtggtgtttt taatgaatgg cggtggccag gccctgattg
     2581 gcgcgtcatt tggcgggcaa cgccagcggc tggccgaatt ggccatctcg ctgaccggca
     2641 gtggcggcct ggccgaatcg gtgttgcagc gcagcctggt ttttgtcagc caggagcgcg
     2701 gcttgctgag cctggccgaa gaccagttgc tgcgggtttt tggcacgcaa tgtctgattt
     2761 gcctgccaat tgcctccagt acccgcagcc ttggcttgct ggtcggcagc gtcgagtcct
     2821 ggcgtattgc cgagctgaaa cggcaggaga aatttttgct ggcctttggt gcgcaagcgg
     2881 cgcaggcgct ggaaaactcg gcccgggagc gtggcgagat tgaccggcgc attgcccggc
     2941 tgcgcgatga gcatttgctc gactcacgca aggtggcaca tgaggtcaac aacccgctgg
     3001 ccatcatcaa aaattacctc ggcgtgctcg atgacaagct ggcacgcgac gagccggtcg
     3061 cggccgaact gtccattctg cacggggaag ttgaccgtgt tggcagcatc atcaagcaat
     3121 ttgccggcat cacaccagcg ccaccggaca gcgtggtgga gatcaaccag gtgatcaacg
     3181 acatcgtgcg gctgtttcgt ggcagccggt ttttgcctgc gtcggtacag atcatcgcgc
     3241 agttgccggg gcagtcctgc gagatcaccg gcgtggccag cgtcctcaag caaatattcg
     3301 ccaatctgat caagaacgcg gtagaagcca tgcccaaggg cgggcggatc gaggtgatca
     3361 accggggtgc ggtgcgacgt gaccggattg actatttcgc gatgctggtc cgggacaccg
     3421 ggccaggcct gcctgatgcg gtcatggcca ggctgttttc gccggtgcgc agcagcaagc
     3481 cgggcgagaa ccgtgggctg ggcctgagca tcgtgcacga cctggtgcaa aaactccatg
     3541 gacaaatcaa ctggaacagc agcaatcttg gcaccgcctt tgaaattctg ttgcccaggc
     3601 gacccagcaa atcagccaac taaatgaagc tcaatgatcc aacccgtcgg ggctgtccct
     3661 tgaccagtga cagtctgatc ccacccaagg ccgtgccaac accagcgcaa ctgctgctgg
     3721 tcgatgacga gccgctgatg ctcgccagtt tgtgcgaact gctgaaacat cgcgactttg
     3781 tgctgaccac cgctgactgc ggtgcggcgg cgattgacct gctgagacac acggtatttg
     3841 acctggtgat cctcgatttg ctgctgcctg atatcagcgg gcagcaggtc atggaattca
     3901 tgaacgcaca gggcattgat accaatgtga tcgtgatcag cggtgccagt ggtatcgatg
     3961 cggccatcgg cgccctcagg gccggtgcct acgactacct gcgcaaaccc tacccgcgcg
     4021 aagagctgct caaagcggtc cagaacgcct tgcaacagca tcggctggcg gcagaaaacc
     4081 ggcagatcgc cgggcagctc gcgagttccg agcggattta ccgctatctg gtggacagtt
     4141 cacccgacat catcttcacg ctcaaccatc agggcatcat cacctttgtc aatgaccggg
     4201 tgcatccgtt gctggggttc aaccgggaag acctgatcgg gcagcattac gccgaactgg
     4261 tgcatgacca ggacatcgaa cgggccgatt atgtttttgg tgacagccag cgcgaccatt
     4321 gctggtcgcg caacattgaa ctgcggctga agtcgcacga tgttgatgac gaggggcgca
     4381 gctttcgcat tgacctgatg acgatttcac tcgacaacgg gcagcactgt gtgacaaccg
     4441 ggtcaacccc gcgcgacaag ctgggcatct atggtgtcgc ccgcgatatc accgagagca
     4501 agcgcgctga tgaactgatt acctaccagg cctaccacga catcctgacc gacttgccca
     4561 accgggtgct gttcaaggac cgcctgagcc tgtcctt
//
LOCUS       DAOSLL010000389         4576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16684_length_4576_cov_4.746737, whole genome shotgun sequence.
ACCESSION   DAOSLL010000389 DAOSLL010000000
VERSION     DAOSLL010000389.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16684_length_4576_cov_4.746737"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(391..2814)
                     /locus_tag="PLB25_15325"
     CDS             complement(391..2814)
                     /locus_tag="PLB25_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HOE42980.1"
                     /translation="MLSTMPPNAPDAGLLQNLNPEQFAAVTLPNEHALILAGAGSGKT
                     RVLTTRIAWLLQNGQATPGGILAVTFTNKAAKEMMTRLSSMLPLNVRGMWIGTFHGLC
                     NRFLRAHYKLANLPQSFQILDTQDQLSSIKRLYKQFAIDDERYPAKQMQWFISACKED
                     GQRPNMVVAKDPETRKKVEIYQLYEEQCQREGVVDFGELMLRSYELLRDNDPIREHYQ
                     RRFRHILIDEFQDTSKLQYAWIKMLAGTGAHGSVDGAFAPTGCVLAVGDDDQSIYAFR
                     GARVGNMADFAREFGVRHQIKLEENYRSGSNILDSANALISHNSKRLGKNLRTAQGPG
                     EPVRVFEASSDFLEANWMVDEMKQLLREGHARKEIAVLYRSNAQSRVIETALFNAAMP
                     YKVYGGLRFFERAEIKNALAYLRLLENPRDDTSFLRSVNFPPRGIGARSLEQLQDLAR
                     DTGCALSDAVSGLGGKAGHNIGAFLAGIDVLREQTQGLSLRDIIAATLEHSGLIAHYK
                     ADREGADRVENLEELVNAAESFVSIEGFGRDAVALPVDELGNALTQSPASQGLDLNQP
                     LQDLPAPDAATGETLSPLAAFLTHAALEAGDNQAQAGQDAIQLMTVHASKGLEFDCVF
                     ITGMEEGLFPHENSMTDRDSLEEERRLMYVAITRARARLYLSHSQTRMLHGQTRYNMK
                     SRFFDELPEEALKWITPRQSGFGNRPGHSGSGFASGSGAASAYMTRATSQFGTNSSGH
                     SAPVMPTRKESGGHSFKVGQKLFHTKFGEGTVLMLEGSGDDARAQINFPRHGVKWLAL
                     SVAKLSPVP"
     gene            2839..3651
                     /locus_tag="PLB25_15330"
     CDS             2839..3651
                     /locus_tag="PLB25_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42981.1"
                     /translation="MTPTLQMRATRPWALSVGWLVEHGFGLKRRPWPTRPWALTGCLL
                     LAALASPLAHSQSACSNDGQPAPGVLFERFINADCAACWRDPATPTAPPDALALDWIV
                     PGSQGDDAALAAASSSDALARLAELQRLRPATQDHQASAVTAWPGAALRLAHGTAVGN
                     YIGVLISLSVPQSASLALPPTGWVVLLEALPSGTEASPVPRYLVRNALQPLWDKREQL
                     SNQDRLSFSETRAMSFPNSAQAGRLRLAGWVQDAKGRILTAALSACPAEDKD"
     gene            3769..>4576
                     /locus_tag="PLB25_15335"
     CDS             3769..>4576
                     /locus_tag="PLB25_15335"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HOE42982.1"
                     /translation="MEIFDYDNILLLPRKCRVESRSECDAGVDFGPRRFRIPVVPANM
                     KTVVSEAICVWLAQNGYFYVMHRFDLDNVRFVKDMAARGLYASISLGVKKPDYDAVDQ
                     LVALGLAPDYITIDIAHGHADSVKNMIGYLREKIPGTFIIAGNVATPEAVIDLENWGA
                     DATKVGIGPGKVCITKLKTGFGTGGWQLSALKWCARVATKPIIADGGIRDHGDIAKSV
                     RFGATMVMIGSLFAGHEESPGQTVEVDGKLYKEYYGSASDFNKGEYKHVEG"
BASE COUNT          849 a         1363 c         1429 g          935 t
ORIGIN      
        1 ctgtgcgccc aaatccgccg gtgcaaatag gggaagttat ttctgaccac atccttagcc
       61 gcgtttgaac gcgccgcaac catgccgctt gctgacaggc gatgccagcg gccaggcacc
      121 gcgcgctcgc gccaggtcat gtgtcgctgg ttgcgacacg tttggccaca ttggtgggcc
      181 aatctgccag ccaggtttgc gccgggcggt cagcctgtgg cattgagatt gtcgggatac
      241 ctggggcgtg cgccaggttc gctgctcatt gcattgccaa aggagccagg ccgttgttat
      301 ctcgccttgc gtttgccatg tctgcgccac gatgcctggc cacgactgat cttgaaatgc
      361 aatcagctcg attgcctggg caatccaggt tcaaggcact ggcgacagct tggccacgct
      421 gagcgccagc cacttgacac cgtggcgcgg gaagttgatc tgggcacggg catcgtcgcc
      481 actgccctcc agcatcaaca ccgtgccttc gccaaatttg gtgtggaaca gcttttgacc
      541 gaccttgaag ctgtggccgc cagactcctt gcgggttggc atcacaggcg ctgaatgccc
      601 tgaagaattc gtaccaaatt ggcttgtagc ccttgtcatg taagcgctgg cagctcctga
      661 acctgaagca aatccagacc cggagtggcc tggccggtta ccaaagccgg actgcctggg
      721 cgtgatccac ttcagcgcct cttcgggcag ttcgtcaaaa aaccggctct tcatgttgta
      781 gcgggtctgg ccgtgcagca tgcgggtttg tgaatggctc aggtacaggc gggcgcgggc
      841 gcgggtgatg gccacgtaca tcagacggcg ttcttcttcc aggctgtcac ggtcagtcat
      901 cgagttttca tgcgggaaca ggccctcctc catgccggtg atgaagacac agtcaaattc
      961 cagccccttg ctggcgtgca cggtcatgag ctgaatcgca tcctgcccgg cttgtgcctg
     1021 gttgtcgccg gcttccagcg ccgcgtgggt caaaaaagcc gccagcggcg acagggtttc
     1081 gccagtggca gcatcgggcg ctggcaggtc ctgtagcggc tgattcaggt cgagcccctg
     1141 cgaggcgggc gactgggtca gggcattgcc caattcatcg accggcaagg cgaccgcgtc
     1201 ccggccaaaa ccttcaatgg atacaaagct ttcggcagcg ttgaccagtt cttccaggtt
     1261 ttcaacccgg tcggcaccct cgcggtcagc cttgtaatgg gcgatcaggc cgctgtgctc
     1321 cagtgtggcg gcaatgatgt cacgcaggct caggccctgc gtttgctcgc gcagcacgtc
     1381 aatgcctgcc agaaatgcgc cgatgttgtg gccggccttg ccacccaggc cactgaccgc
     1441 atcgctcagg gcgcaaccgg tgtcacgcgc caggtcttgc aactgctcca ggctgcgtgc
     1501 gccaatgccg cgtggcggaa agttgaccga gcgcaaaaag ctggtgtcgt cgcgcgggtt
     1561 ttccagcagg cgcaggtagg ccagcgcgtt cttgatttca gcccgctcaa aaaaccgcag
     1621 gccaccatag accttgtaag gcatggcggc gttgaacagc gcagtttcaa tcacccggct
     1681 ttgggcattg gagcggtaca gcacggcgat ttccttgcgc gcatggcctt cgcgcagcaa
     1741 ctgcttcatt tcatcgacca tccagttggc ttcaagaaag tcgctgctcg cctcaaacac
     1801 ccgcaccggc tcacccggac cctgagcggt gcgcaggttt ttgcccaagc gcttgctgtt
     1861 gtggctgatc agcgcgttgg cgctgtcgag gatgttgctg ccgctgcggt agttttcttc
     1921 cagcttgatc tggtggcgca caccaaattc acgcgcaaag tcggccatgt tgcccacccg
     1981 tgcgccgcga aaggcgtaaa tgctctggtc atcgtcgccc accgccagca cgcagccagt
     2041 cggcgcaaat gccccatcaa cactgccgtg cgcgccggtg ccagccagca tcttgatcca
     2101 ggcatattgc agtttgctgg tgtcttgaaa ctcgtcgatc aggatgtgcc tgaagcggcg
     2161 ctggtagtgc tcgcggatcg ggtcgttatc gcgcagcagt tcatagctgc gcaacatcag
     2221 ttcgccaaaa tcgaccaccc cttcgcgctg gcattgctcc tcgtagagtt ggtagatttc
     2281 gacctttttg cgcgtctcgg ggtctttggc caccaccatg ttgggccgct ggccgtcctc
     2341 cttgcaggcg ctgatgaacc attgcatctg tttggccggg tagcgttcgt cgtcaatggc
     2401 aaactgcttg tataggcgct tgatgctgga gagttggtct tgcgtgtcga gaatctgaaa
     2461 gctctgcggc agattggcca acttgtaatg tgcccgcaaa aaccggttgc acaggccgtg
     2521 aaaagtgcca atccacatgc cgcgcacgtt caggggcagc atgcttgaca ggcgtgtcat
     2581 catctccttg gcggccttgt tggtaaaggt caccgccaga atgccgcccg gcgtcgcctg
     2641 gccgttttgc agcagccagg caatgcgggt ggtcagcacc ctggttttgc cggaaccggc
     2701 gccagcgagg atcagcgcgt gttcgttcgg cagggtgacg gcggcaaact gctcagggtt
     2761 aaggttttgc agcaggccgg catccggggc gttggggggc atggtagaaa gcatggcttg
     2821 attgtagaaa ggcgaccgat gacccccact ttgcaaatgc gcgccactag accgtgggcg
     2881 ctgtccgtcg gctggcttgt agaacacggc tttggcttga agcggcgacc atggcccacc
     2941 cgaccatggg cgctgaccgg ctgcttgcta ctggcggctc tggcatctcc gctggcgcac
     3001 agccagagcg cctgcagcaa tgacggtcag cccgcgcccg gcgtgctgtt cgagcgcttc
     3061 atcaatgctg actgcgcagc ctgctggcgc gacccggcca cgccaacggc cccacctgat
     3121 gcgctggcat tggactggat cgtgccgggc agccagggcg acgatgcggc gctggcagca
     3181 gccagcagca gtgatgcgct ggcgcggctg gccgagctgc agcgtctgcg ccccgccacg
     3241 caagaccatc aggcaagcgc cgtcacggcc tggccagggg cggccttgcg tctggcgcat
     3301 ggcacggcgg ttggcaatta catcggcgtg ttgatctcgc tgagcgtgcc gcaaagcgcc
     3361 tcgctggcct tgccaccgac cggctgggtg gttttgctgg aagcactgcc gagcggtaca
     3421 gaggcttcac cggttcccag atatttggtc agaaatgcac tgcagccctt atgggataag
     3481 cgcgagcagc tatcgaatca ggatcgtttg agtttcagcg aaacgcgtgc catgagcttt
     3541 ccgaacagcg cccaggcggg tcggctgcgg ctagcgggct gggtgcagga cgccaagggc
     3601 cggattctga ccgccgcact gtctgcttgc ccggcagaag ataaagactg acccggatgg
     3661 acgaacccga tagaatcagg caccgggccc aagcaattgc agcccggttt ttttgcgccg
     3721 gttgccatcc aagcgctctt gtatcaaacc tcagaaaggc gctggaacat ggaaattttt
     3781 gactacgaca atattttgct gctgccacgc aaatgccggg tggaaagccg ctcggagtgt
     3841 gatgcggggg ttgactttgg cccgcggcgc tttcgcattc cggtggtgcc agccaacatg
     3901 aaaaccgtgg tcagcgaggc catttgtgtc tggctggcgc aaaacggcta cttctatgtg
     3961 atgcaccgct ttgaccttga taacgtgcgg tttgtcaagg acatggcggc gcgcggcctg
     4021 tatgcatcga tctcgctggg cgtgaagaag ccggactatg acgcggtgga ccaactggtg
     4081 gcgctgggcc tggcccccga ttacatcacc atcgacattg cccatgggca tgccgacagc
     4141 gtgaaaaaca tgatcggtta cctgcgtgaa aagatccccg gcaccttcat catcgccggc
     4201 aatgtggcca cgccagaggc cgtgattgac ctggaaaact ggggtgccga tgccaccaag
     4261 gtcggcattg gccctggcaa ggtctgcatc accaagctga aaaccggctt tggcaccggt
     4321 ggctggcaac tgagcgcgct gaaatggtgt gcccgggtgg cgaccaagcc catcattgcc
     4381 gatggcggca ttcgcgacca tggcgacatt gccaaaagcg tgcgttttgg cgcgaccatg
     4441 gtgatgatcg gctcgctgtt tgccggtcac gaagaaagcc ccggccagac ggtggaggtc
     4501 gatggcaagc tgtacaagga atactacggc tcggccagcg acttcaacaa gggcgagtac
     4561 aagcacgtgg aaggca
//
LOCUS       DAOSLL010000390         4576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16687_length_4576_cov_4.288874, whole genome shotgun sequence.
ACCESSION   DAOSLL010000390 DAOSLL010000000
VERSION     DAOSLL010000390.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16687_length_4576_cov_4.288874"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="PLB25_15340"
     CDS             complement(<1..907)
                     /locus_tag="PLB25_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42983.1"
                     /translation="MTMPMTLWNKIGLNLKLQLLIQGFLIVILVSAQHWVSRYIEDQL
                     LNAARERTMVVGDGVINGLNTMMVAKMTILDPEARALFIKKMGASETIKEVRVVRGKG
                     LIDQYGAGLPQEQAVDELDRRVLSTGLMEFQTVHSANGDVSLRAALPFIAKKDFRSSN
                     CLTCHAVPENTVLGMTSITVDINNDIAKIAQVNRLMWLGQLVIQIVLFFVIGLIVRSL
                     LRKLGGEPNYVIDILEQITRGNLSSDIRTRSNDSISLLANVKIMQGGLRKVVDEMQAV
                     VTAAAHGDLSKRIRLDDKHGFAKDLS"
     gene            1367..2293
                     /locus_tag="PLB25_15345"
     CDS             1367..2293
                     /locus_tag="PLB25_15345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HOE42984.1"
                     /translation="MASLKSTTKSLQVLQGVRILSVSLNLPGPAALLRCKKMGATCVK
                     LEPPSPVDAPEGTTGDPMSVYSRRAYDALHAGIRVKTINLKSATGQKALQRELAQAQV
                     LLASFRPSALTKLGLDWQTLHPRYPALAMVSIVGSAGGRAEEPGHDLTYLADNDLVTG
                     LDLPATLYADMGGALMACEAVLQAVLMQKQSGQGSFIEVALADATAHLALPRTWGATL
                     PGTLLGGGHAGYQVYPCLNGRVAMAALEPHFARTLCAVAGIEWTGMAMMSEASTRQKV
                     ADFVRKLTQTELNLLAKAHDIPLHTLQPDLPG"
     gene            complement(2386..3951)
                     /gene="malQ"
                     /locus_tag="PLB25_15350"
     CDS             complement(2386..3951)
                     /gene="malQ"
                     /locus_tag="PLB25_15350"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HOE42985.1"
                     /translation="MYFDFQTRRCGVLLHPTSLPGPHGSGDLGPNAYRFVDWLQSAGQ
                     TLWQVLPLNPIGGAGHSPYSGVSAFAGNPLLIALEPLTEHGWLAADLNVDTSQFNDLC
                     VNFGHVVAWRMQQLHSAWQGFCRCASDTDQLAFRGYCESEKAWLEDYALFMALDQIHN
                     PPDQAFRPWCEWDSALAQRDPQTLQAARLELAGPVFFWQFVQWCFDTQWQALRRYARE
                     HQVFIVGDMPIFIAHHSADCWARPDLFLLDAQGQPSVVAGVPPDFYSPTGQRWGSPLY
                     RWDVFQQEGFAWWIARIQRQLALSDVIRIDHFRGFAACWEIPATCPTAEHGNWMPAPG
                     RALFTAIQEALGKLPIIAEDLGIITPDVVELREAFSFPGMRILQFAFSETADNAFLPH
                     NYTPCTVAYTGTHDNDTVLGWWSSATEREQAFARIYLGGEEPQSHWAMIRAVLASVAQ
                     TVIVPMQDVLGLDGRHRMNTPGQLGAWHWRFAWDWLDHATTETLGSLSATYGRAPIDR
                     LQLPAYPVGQPHP"
     gene            complement(4293..>4576)
                     /locus_tag="PLB25_15355"
     CDS             complement(4293..>4576)
                     /locus_tag="PLB25_15355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HOE42986.1"
                     /translation="TAPTSGTVIALDPDIPPQRQRVSFSAQGSNLHWLMDGRQFAKGP
                     TAQWLPWPGRHLVQLADGRGQVQDEIRLEVRGAGVKAPQRRPQTTPERQ"
BASE COUNT          974 a         1347 c         1330 g          925 t
ORIGIN      
        1 tgctcagatc tttggcaaaa ccatgtttgt catcaagcct gatacgtttg gacaagtcgc
       61 cgtgcgcagc ggctgtcacc actgcctgca tttcatcgac caccttgcgc aaaccgcctt
      121 gcatgatttt cacgttcgcc agcaggctga tcgagtcgtt gctgcgcgtg cggatgtcgc
      181 tggacaggtt gcctctggtg atctgttcca gaatgtcaat cacgtaattc ggctcgccgc
      241 ccaattttcg caggagactg cgaacgatca ggccgatgac aaaaaacagc acgatttgaa
      301 tcaccagttg cccaagccac atcagccggt tgacctgcgc aattttggca atatcgttgt
      361 tgatgtccac cgtgatgctg gtcattccca aaacagtgtt ttcgggcacg gcgtggcaag
      421 tcaggcaatt ggacgagcga aaatctttct tggcgatgaa cggcagcgcc gccctgagcg
      481 acacatcacc attggcggaa tgaaccgtct ggaattccat gagcccggta ctcaaaacac
      541 gccggtccag ctcgtcaacg gcttgctcct ggggtagtcc tgccccatac tgatcaatca
      601 gacccttgcc tcggacaacc cttacctctt tgattgtttc cgaagcgccc atcttcttga
      661 tgaacagcgc ccgggcttcc ggatccagaa tggtcatctt ggccaccatc atggtgttga
      721 gcccattgat gacaccgtcg ccgacaacca tggtccgttc ccgcgccgca ttgagcaact
      781 gatcctcaat gtagcgggaa acccagtgct gggcagaaac cagaatcaca atcaaaaagc
      841 cctgaatcag caattgcaat ttcagattca ggccgatttt gttccatagc gtcattggca
      901 tagtcatggc agatccaaga aatgaaagat ggcagcgcta ggtccctgat gttcagcgcc
      961 agcacgtccg gatctgcgcg ctggtttcgc gcgcaagatt tttcgtagcg gacaaatcag
     1021 tataaatctt gcaggatgcg tgtcagccca gctatcgggc gattgggccg ctggcgcgcc
     1081 aactgttttt tttggcatct tcaacgggtt gactttgcct atcgactgct gcctgcgacc
     1141 atttgggatg cacgctggct ggcctctttc tgaccggatg gaatcgaatc cttttgggtg
     1201 ccggagtttc ataagtattt ggcctccaga ccttatgaaa taagcgctga tagctacgat
     1261 attaatagct atttgttcca tcaaggccag gcaacggcgt gaacaaatcc gaatcaggat
     1321 ggctgaaatt tgttgtaatc gggcttttga atcacacaaa cctgccatgg caagcctcaa
     1381 atcaacgacc aagtccctgc aagtgctgca gggtgtgcgc attctcagcg tcagcctgaa
     1441 tctgcccggc ccggccgcct tgctgcgctg caaaaaaatg ggtgccacct gcgtcaagct
     1501 tgaacccccg agcccggttg acgcacctga aggcaccacc ggcgacccga tgagtgttta
     1561 cagccgccgt gcctacgatg ccttgcacgc tggcattcgc gtcaagacca tcaacctgaa
     1621 atcggccacc ggacaaaagg cattgcagcg cgaactggca caagcccagg tgttgctggc
     1681 atcatttcgc ccgagtgccc tgaccaagct cggactggac tggcagacac tgcacccgcg
     1741 ctacccggcg ctggccatgg tctcgattgt gggttctgct ggcgggcgtg ccgaggagcc
     1801 cggccacgac ctcacctatc tggccgacaa cgatctggtc accggtctgg acttgcccgc
     1861 caccttgtat gccgacatgg gcggcgcact gatggcctgc gaggcagtgc tacaggcggt
     1921 gctgatgcaa aagcagagcg ggcagggcag tttcattgag gtcgcgctgg cagatgcaac
     1981 ggctcatctg gcgttgccgc gcacctgggg tgccaccttg cccggcaccc tgctgggcgg
     2041 tggccatgcc ggttaccagg tttacccctg cctgaacggc cgggtggcga tggcggcact
     2101 cgaaccacac tttgcgagaa cactttgcgc ggtagccggt attgaatgga cgggcatggc
     2161 catgatgtca gaagcgtcca cccgtcagaa agtggccgac tttgtccgca aactgacaca
     2221 gaccgagctg aacctgctgg ccaaggcgca cgacattccg ctgcatacgc ttcaacctga
     2281 cttgcccggt tgagaagtcc gggttcatgc agcggtttga ccggcggtgc ggcaacccgg
     2341 gcttggctca atgcaagtga ctaacgcgcc acgcttcggc gctgctcaag gatggggctg
     2401 accgaccgga taggctggca attgcaggcg gtcaattggt gcacggccat aggtggcgct
     2461 cagcgagcca agcgtttcgg tggtggcgtg gtcgagccag tcccaggcaa atcgccagtg
     2521 ccaggcacca agctggccag gtgtgttcat gcggtggcgc ccgtccaacc ccagcacgtc
     2581 ctgcatcggg acgatgacgg tttgcgcgac cgatgccagc actgcacgga tcatcgccca
     2641 gtgcgactgc ggttcttcac cgccgaggta aattcgggca aaggcttgct cacgctcggt
     2701 agcgctgctc caccacccca gcactgtgtc gttgtcatgg gttccggtgt aagccactgt
     2761 gcatggtgtg tagttgtgcg gcaaaaaggc gttgtctgcg gtttcgctaa aggcgaactg
     2821 aagaatgcgc atgcccggaa aagaaaacgc ttcacgcagt tccaccacat ccggcgtgat
     2881 gatcccgagg tcttctgcaa tgatcggcag tttccccagc gcctcctgga tggccgtgaa
     2941 caaggcgcga cccggtgctg gcatccagtt cccatgttcg gctgtggggc aagttgctgg
     3001 aatctcccaa caggcagcaa agccacgaaa gtggtcaatc cggatcacat cggacagcgc
     3061 aagctggcgt tggatgcggg caatccacca ggcaaaaccc tcttgctgaa aaacatccca
     3121 gcggtacagc gggctgcccc aacgctggcc ggtcggcgaa tagaagtccg gcggaacgcc
     3181 cgccaccacc gacggttgtc cctgcgcatc aagcaaaaac aggtccggtc gggcccagca
     3241 atcggcactg tggtgcgcga taaaaatcgg catatcacca acgataaaaa cctggtgctc
     3301 ccgggcgtag cggcgcagcg cttgccactg ggtgtcaaaa caccattgga caaactgcca
     3361 gaaaaagacc ggtccagcca gctcaagccg ggccgcttgc agggtctggg ggtcgcgttg
     3421 tgccagggcg ctatcccact cgcaccaagg gcggaaagcc tgatccggcg gattgtggat
     3481 ctggtcgagt gccatgaaca gtgcgtaatc ttccagccag gctttttcgc tctcgcaata
     3541 gccccgaaag gcaagctggt cggtgtccga cgcacaacga caaaacccct gccaggcgct
     3601 gtgcagttgc tgcatgcgcc aggcaacgac atggccgaag ttcacgcaca ggtcattgaa
     3661 ctggcttgtg tcaacgttca ggtctgcggc cagccagccg tgctcggtca acggctccag
     3721 ggcaatcagc agcggattgc cggcaaaggc ggaaacgccc gaatagggtg agtggcccgc
     3781 gccaccgatc gggttaaggg gtaatacttg ccacagggtc tggccggcgc tttgcagcca
     3841 gtcaacaaag cgataggcat tggggcccaa gtcgccgctg ccatgcggcc ccggcaaact
     3901 ggtgggatgc agcaaaaccc cgcatcgacg tgtttgaaaa tcaaagtaca caaagactcc
     3961 agcaattcac acaggacata caaaggccgg gctgaattct atgtagtctg accagaaatc
     4021 acatcagatt tttcgaaagt tcttgtaact ctcaaaaatc cgcaggtttg tgcttcgggt
     4081 agcgtcgatt gcaaacaatc gcaccgggtt acccgtgcaa catggcagta ccagctttcg
     4141 ggcgatgcca ttgcctggct gcaaggcaag cctggagaga aaatgtggct gcgggcgcga
     4201 aagattggga tctggacaag tgaacgggca agggttgcgg cctcagccca ggcgttgccg
     4261 aattcgtcgc cctgccgcca ggcaagctgg catcattgcc gctcaggtgt ggtttgcggc
     4321 ctgcgctgtg gcgctttgac accagccccg cgcacttcga gccgtatctc gtcctgcacc
     4381 tggccacgtc catcagccaa ctgcaccaga tggcggcccg gccagggcag ccactgtgcg
     4441 gtcgggcctt tggcaaactg tcggccatcc atcagccagt gcaggttgct gccctgtgcg
     4501 ctgaaactga cgcgttggcg ctgcggcgga atgtcggggt cgagcgcgat cacggtgccg
     4561 ctggtgggtg cggtga
//
LOCUS       DAOSLL010000391         4576 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16688_length_4576_cov_3.901128, whole genome shotgun sequence.
ACCESSION   DAOSLL010000391 DAOSLL010000000
VERSION     DAOSLL010000391.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4576)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4576
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16688_length_4576_cov_3.901128"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1361)
                     /locus_tag="PLB25_15360"
     CDS             complement(<1..1361)
                     /locus_tag="PLB25_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012839376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOE42987.1"
                     /translation="MTDSISQPGLDSLSKSFEPAALEAHWGPEWEKRGYGVAGYRGSS
                     APDAQAAARGDNFCIQLPPPNVTGTLHMGHAFNQTIMDSLTRYHRMAGFNTAWIPGTD
                     HAGIATQIVVERQLQAQGISRHDMGATPFEARQNFVAKVWEWKEKSGNTITTQMRRMG
                     DSVDWSREYFTMDEKLSQTVTETFVRLYEQGLIYRGKRLVNWDPVLMSAVSDLEVESE
                     EEDGFLWHILYPFADGPQLVNGELAQGLVVATTRPETMLGDVAAMVHPEDERYTHLLG
                     KQVTLPLCNRTIPIIADDYVDKAFGTGVVKVTPAHDQNDYAVGQRHQLPMICVLTLDA
                     KINTQAPAVYQGMDRFAARKQIVIDLQALELMVEVKKHKLMVPRCARTGQVIEPMLTD
                     QWFVAMSKVSPQDPTGKSITQKAIDAVQSGAVKFVPENWVNTYNQWMNNIQDWCISRQ
                     LWWG"
     gene            complement(1482..3044)
                     /gene="ahpF"
                     /locus_tag="PLB25_15365"
     CDS             complement(1482..3044)
                     /gene="ahpF"
                     /locus_tag="PLB25_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HOE42988.1"
                     /translation="MLDLDLQTQLAAYLQRVTLPFEIVASLSDSTSSRELQQLLQQIQ
                     GMSDKISLRTDGTDARKPSFTLQRSGSATSLRFAAIPLGHEFTSLVLALLWTGGHPPK
                     VEPAIIEQIKALDGDFNFEVYMSLSCHSCPDVVQALSLMAIHNPCVKVVVIDGALYQD
                     EVNQRQVMAVPMVFLNGEMFSSGRVLIEEILAKLDSGAAARDAAKLSAAAPYDVLIVG
                     GGPAGAAAAVYAARKGIRTGIAAERFGGQVNDTNAIENFISVLETDGPKFAAGLEAHV
                     RAYGVEVMNLQTADKLVPAAQPGGLITLQLANGGTLKSKTVILSTGARWRNVNVPGEQ
                     QYRNKGVAYCPHCDGPLFKGKHVAVIGGGNSGVEAAIDLAGVVAHVTLIEFDTQLRAD
                     AVLVNKLKSLSNVTIHVHAQTTEITGDGSKVNGLNFTERATGNARHIDLQGVFVQIGL
                     VPNTEWLKGTLELSRHGEIVVDAKGATSLPGVFAAGDATTVPFKQIIIAAGDGAKAAL
                     SAFDYLIRTPVA"
     gene            complement(3210..3773)
                     /gene="ahpC"
                     /locus_tag="PLB25_15370"
     CDS             complement(3210..3773)
                     /gene="ahpC"
                     /locus_tag="PLB25_15370"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017329783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HOE42989.1"
                     /translation="MSLINTQVKPFKAQAYQAGKFFEVTEQSLLGKWSVLVFYPADFT
                     FVCPTELEDLADAHAEFAKVGVDIYSVSTDTHFAHKAWSDTSPAIKKVQYAMIGDPTG
                     TITRNFDVMIEEAGLALRGTFVINPEGVIKVCEIHDLGIGRDAMELLRKVKAAQYVAT
                     HNGEVCPAKWKEGQATLKPSLDLVGKI"
     gene            4000..>4576
                     /locus_tag="PLB25_15375"
     CDS             4000..>4576
                     /locus_tag="PLB25_15375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HOE42990.1"
                     /translation="MLFLLSPAKALDFEPSEPALPHSQPQFVPQAAELIGILKQLSPP
                     QVARLMSLSDALSALNVARYQAWSPKFTRKNAKQAVLAFNGDAYQGLAAGTLALEDLN
                     WLQQHLCILSGLYGVLRPLDYLQPYRLEMGTQLPNARGKNLYEFWGSRISEHLNARLK
                     SEVAPVLVNLASQEYFKAVDLKILKARVIDCV"
BASE COUNT          907 a         1342 c         1349 g          978 t
ORIGIN      
        1 ccccaccaga gctggcggct gatgcaccag tcctgaatgt tgttcatcca ctggttgtag
       61 gtgttgaccc agttttccgg gacgaacttg actgcacctg actgcaccgc gtcgatggct
      121 ttttgggtga tcgacttgcc ggtggggtcc tgggggctga ccttgctcat ggcgacaaac
      181 cattggtcgg tcagcattgg ctcaatcacc tggccggtgc gggcgcagcg cggcaccatc
      241 agtttgtgtt ttttgacttc gaccatcagc tccagggcct gcaggtctat gacgatttgt
      301 ttgcgtgcgg caaatcggtc catgccctga tacaccgcag gggcttgcgt gttgatcttg
      361 gcatccaagg tcagcacgca gatcatcggc aactggtggc gctggcccac cgcgtagtcg
      421 ttttggtcgt gtgccggggt gaccttgacc acgccggtac caaaggcctt gtccacatag
      481 tcgtcggcaa tgatcggaat ggtgcggttg cacagcggca aagtgacctg tttacccagc
      541 agatgggtgt aacgctcatc ttcaggatgc accatggcgg caacgtcgcc cagcatggtc
      601 tccgggcgcg tcgtggccac caccagaccc tgcgccagtt cgccgttcac caactgcggg
      661 ccgtcggcaa acgggtacaa gatgtgccag agaaagccgt cttcctcctc gctttcaact
      721 tccagatcag acacggcgct catcaacacc gggtcccagt tgacgagccg tttgccacgg
      781 tagatcaggc cctgctcgta caggcgcaca aaggtttctg tcacggtttg cgacaacttc
      841 tcgtccatgg tgaagtattc gcggctccag tccacgctgt cgcccatgcg gcgcatttgc
      901 gtcgtgatgg tgttgccgct tttctctttc cattcccaga ccttggcaac aaagttctgg
      961 cgcgcctcaa atggcgttgc gcccatgtcg tggcggctga tgccctgggc ttgcaactgg
     1021 cgctcgacca caatttgcgt ggcgatgccg gcatggtcag tgccgggaat ccaggcggtg
     1081 ttaaagcctg ccatgcggtg gtaacgcgtc aggctgtcca tgatggtctg gttgaacgca
     1141 tggcccatgt gcagcgtgcc agtcacgttg ggcggcggca actggatgca gaagttgtcg
     1201 ccgcgtgcgg cggcctgtgc gtcgggcgcg ctgctgcccc ggtagccagc caccccgtag
     1261 ccgcgctttt cccattccgg gccccaatgg gcctcaagcg cggcaggctc aaaggacttg
     1321 gacaggctgt ctaggccggg ttgcgagatg gagtcggtca tgggatggcg ccaaaaatga
     1381 cgagcggtgc cagtttgggc accgctcggt ggaacaaaag aggggaactg ccccggattt
     1441 taccgagaca gtgcgccgcc tctggcaccc tggcaagccg gtcacgccac tggcgtgcga
     1501 atcaggtagt caaacgcgct gagcgcagcc ttggcgccat cgccggcggc gatgatgatc
     1561 tgcttgaacg gcacggtggt ggcatcacca gcggcaaaca cgccgggcaa cgaggttgca
     1621 cctttggcat ccaccacaat ttcgccatgg cgcgacaact ccagcgtgcc cttgagccat
     1681 tcggtattgg gcaccaggcc aatctgcaca aaaacgcctt gcagatcaat gtgcctggcg
     1741 ttgccggtgg cacgttcggt aaagttcagg ccgttgacct tgctgccgtc accagtgatc
     1801 tcggtcgttt gggcgtgcac atgaatcgtc acgttggaca ggcttttgag cttgttgacc
     1861 agcaccgcgt cagcgcgcaa ctgggtgtca aactcgatca gggtgacatg cgccaccacc
     1921 ccggcgaggt caatggcagc ttccacccct gaattgccgc caccaatcac cgccacatgc
     1981 ttgcccttga acagcgggcc gtcgcagtgc gggcaatagg cgacaccctt gtttctgtat
     2041 tgctgctcgc ccggcacatt gacattgcgc cagcgcgcac cggtcgacag aatcacggtc
     2101 ttgctcttga gcgtgccgcc attggccaat tgcagcgtaa tcaagccgcc tggctgcgct
     2161 gctggcacca acttgtcggc ggtttgcagg ttcatgacct ccacaccata agcccgcaca
     2221 tgcgcttcga gcccggcggc aaatttgggg ccatcggttt ccagcaccga aataaagttt
     2281 tcaatcgcat tggtgtcatt gacctggcca ccaaagcgct cggctgcgat gccagtgcga
     2341 atgcctttgc gcgcagcata aactgccgcc gccgcgccag ccgggccgcc gccaacgatt
     2401 aacacgtcat agggtgctgc ggcgctgagt ttggcggcat cgcgggcagc agcgccactg
     2461 tcgagcttgg ccagaatctc ctcgatcaag acgcggcccg agctgaacat ctcgccgttc
     2521 aaaaacacca tcggcactgc catcacctgg cgctggttca cttcgtcctg gtacagcgcg
     2581 ccatcaatca ccaccacctt gacgcacggg ttgtgaatgg ccatcaggct cagggcctgc
     2641 accacgtccg ggcaactgtg gcacgacagt gacatataga cttcaaaatt gaagtcaccg
     2701 tcaagcgcct tgatctgctc gatgatggct ggctcgacct tgggcgggtg accaccggtc
     2761 cataacagcg ccagcaccag tgaagtgaat tcatgaccaa gcggaatcgc ggcaaagcgc
     2821 agactggtcg cactgccgct gcgctgcagg gtgaaagagg gtttgcgggc atcggtaccg
     2881 tcggtacgca ggctgatctt gtcagacatg ccctgaattt gctgcaacag ttgctgcaat
     2941 tcgcgcgagc tggtcgaatc gctcaggctg gcaacgattt caaacggcag ggtgacgcgc
     3001 tgcaggtagg cagccagttg ggtttgcagg tcaaggtcga gcatgaaggt ctccgaaaaa
     3061 ctataaaaaa tagagcatgt tgcgcttgtg gcacaagggc tagaggtggt ttttggccga
     3121 aaaaaagccg gacggcaggg caactggtgc accctgacgc agtccggcat tgggtgtacc
     3181 gcccgggtac acccgttgct gcacggtact tagatcttgc cgaccaggtc cagcgatggc
     3241 ttcagggtgg cttgtccttc cttccacttg gccgggcaaa cttcgccgtt atgggttgcc
     3301 acatactggg ctgccttgac cttgcgcagc agttccatgg catcgcggcc aatgccaagg
     3361 tcatgaattt cgcaaacctt gatgacgcct tccgggttga tcacaaacgt gccgcgcagt
     3421 gccaggccag cctcttcgat catcacgtca aagttgcggg tgatggtgcc ggtcgggtcg
     3481 ccaatcatcg cgtactgcac cttcttgatg gccggtgatg tgtcggacca ggccttgtgg
     3541 gcaaagtggg tgtcggtcga gacgctgtaa atgtcgacgc ccaccttggc aaattcggca
     3601 tgcgcatcgg ccaggtcttc gagttcggtc gggcagacaa aggtgaagtc agccgggtaa
     3661 aacaccagca ccgaccattt gcccaaaagg gactgttcgg tgacttcaaa aaacttgcca
     3721 gcttgatagg cttgggcttt gaatggtttg acttgggtat tgatcaagga catatgaaac
     3781 tcctgagttg acgtgttgaa aaagcgggtt aagaaaacgt cggctaaaca gattgttctt
     3841 gccgatgggt gaatcatagc ggccacctat cgttttcatg atgcattgtt tgtaagctat
     3901 tgatagccat tctttatgag tgcagtcgcg ataatgatcc ggccagcagc cattgctgcc
     3961 attgcacccc gttcgccatc tgccattggt caatccatca tgctgttttt actatccccc
     4021 gccaaagccc ttgactttga gccgagcgag cccgcattgc cccacagcca gccgcagttt
     4081 gtgccgcagg cggccgagct gatcggcatt ctcaagcaac tgtcaccacc gcaagtcgcc
     4141 aggctgatga gtctgagtga cgcgttaagc gccctgaacg tggcgcggta ccaggcctgg
     4201 agccccaaat tcacccgcaa gaacgccaaa caggcggtgc tggcgttcaa tggtgatgcc
     4261 taccagggtc tggccgctgg aacgctggcg cttgaagatc tgaactggct gcagcagcac
     4321 ctttgcatct tgagcgggct ttacggtgtg ctgcgcccgc tcgactacct gcagccctac
     4381 cggcttgaaa tgggtaccca gttgcccaac gcgcgcggca aaaacctgta cgaattctgg
     4441 ggctcccgga tttccgagca cctcaatgcc cggctgaaat cagaagtggc accggtgctg
     4501 gtgaacctgg catcacaaga gtatttcaag gcagtggacc tgaaaatact caaggcccgc
     4561 gtgattgact gcgtgt
//
LOCUS       DAOSLL010000392         4569 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16719_length_4569_cov_3.632920, whole genome shotgun sequence.
ACCESSION   DAOSLL010000392 DAOSLL010000000
VERSION     DAOSLL010000392.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16719_length_4569_cov_3.632920"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="PLB25_15380"
     CDS             <1..1415
                     /locus_tag="PLB25_15380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOE42991.1"
                     /translation="TPADLAQELEDRDYYTEVNAFWVPESARWEALRSAAKQSDIGKR
                     IDDALTVIEAENPKLKGILDKRYARAQLPDGKLGELVDLVSTIGFGDNPSTARDVLGQ
                     VYEYFLGMFASAEGKRGGQFYTPASIVKTLVAILSPHSGQVYDPCCGSGGMFVQSEKF
                     IEAHGGKIGDVSIYGQEANPTTWRLAAMNLAIRGIDFNLGREPGDTFIRNQHPDLRAD
                     FILANPPFNISDWWHGSLTGDARWVYGDPPQGNANYAWLQHMLHHLKPTGRAGIVLAN
                     GSMSSSQNSEGTIRAAMVDADVVEVMVALPGQLFFNTQIPACLWFLVKQKTHRKGQVL
                     FIDARKQATMISRVQSELTDAVIERIAATVAAWRGEPDAGGAALGADNHSENDSCSRM
                     EYADIPGFCRSVPLAEIAQHGHVLTPGRYVGAEEVEDNDEDFATKMQQLTEKLGEQMA
                     KGAELDQLIRAKLGGLGYEF"
     gene            1405..2475
                     /locus_tag="PLB25_15385"
     CDS             1405..2475
                     /locus_tag="PLB25_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003487959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOE42992.1"
                     /translation="MSSEVTIHTAECRQWLGELKTRFRQVQLKAAVAVNTAMLQFYWE
                     LGADMVAKQAQFAWGSGFLTQLSADLMREFPDTRGFSKRNLELVRQWHLFWLGAPIGQ
                     QPVAQFAKQPVSQILSIPWGHNLAILAKCKQHDEALYYAQATQTYGWSRSVLVHQIES
                     GLWQREGKAITNFEQTLPAAQSDLATQILKDPYKFDFLSLSKEHTERELETDLIAHIT
                     QFLLELGAGFAYIGRQVPLQVGEREFFLDLLFYHTRLHCHVVVELKTVDFEPEFAGKL
                     NFYIKAVDEQLRGEGDQPTIGLLLCKSKDRLVAEYALSDIQKPMGLSTYTLSHTLPEA
                     LRDKLPSIEAIERELGLDGATP"
     gene            2472..2603
                     /locus_tag="PLB25_15390"
     CDS             2472..2603
                     /locus_tag="PLB25_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42993.1"
                     /translation="MTDNLDQLAGHMVALFRQAVPLANAEVDAIIFKIREQGETQWA"
     gene            2594..3025
                     /locus_tag="PLB25_15395"
     CDS             2594..3025
                     /locus_tag="PLB25_15395"
                     /inference="COORDINATES: protein motif:HMM:NF020360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase substrate binding
                     protein"
                     /protein_id="HOE42994.1"
                     /translation="MGLNVEHLLRTAATLEQAMLALGKTPSADEVIYDLYRNAAIKSF
                     ELSLETAGKLLRKALKAFGGNPRSVDSLVFNDVLRHSGKHAVLDQAEVERWLAYRANR
                     NNTAHDYGVGFANDTLTLLPAYLVDVRALAEKLQKVFDAAA"
     gene            3012..3374
                     /locus_tag="PLB25_15400"
     CDS             3012..3374
                     /locus_tag="PLB25_15400"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HOE42995.1"
                     /translation="MPQRELSTLQLAPQHLQLLQALLHTHVPEAQVWAYGSRVTGGAH
                     EGSDLDLVLRNPADLQHELANWFELKEALQDSQLPLLVELHLWSHLPKTFHPNIEAAY
                     VELQAGRLDLPETDGTSP"
     gene            3371..3670
                     /locus_tag="PLB25_15405"
     CDS             3371..3670
                     /locus_tag="PLB25_15405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42996.1"
                     /translation="MTDNLDQLAGQMVTLFRRAVPLANAEVDAIIQSGERDINRIEHQ
                     LDHMLGFCCAPDMLVVYKRLCRYYDGIDPVAAAGYVQAVREMWDMPDEMVLGGGV"
     gene            3667..>4569
                     /locus_tag="PLB25_15410"
     CDS             3667..>4569
                     /locus_tag="PLB25_15410"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOE42997.1"
                     /translation="MSAEIRPTLLGDLLEITSSKRIHMADYVPSGIPFYRSKEVIERA
                     KGNNISTELFISHKQFDLIKQKFGSPRDGDILLTSVGTLGVPYVVRGDGDFYFKDGNL
                     TWMRNFSKEISPQFLYYWLTSPQIQQKLDEVSIGSTQKALTISALKALQIPLPTRATQ
                     DGIVEILGAISDRITLLRETNATLEAIAQALFKSWFVDFDPVRAKMEGRAPEGMDEAT
                     AALFPDGFEESELGLVPRGWQVATLANAFELNPSRKLPKGSKSLYLDMASLATSGHCV
                     VAPIDRELGSGSKFQNGDTLLARIT"
BASE COUNT         1031 a         1344 c         1316 g          878 t
ORIGIN      
        1 ccacccccgc cgacctggca caagagctgg aagaccgcga ctactacacc gaagtcaacg
       61 ccttctgggt gcccgagtcc gcccgctggg aggcgctgcg cagtgccgcc aagcaaagcg
      121 acattggcaa gcgcattgac gacgcactca ccgtcatcga ggctgagaac cccaagctca
      181 aaggcatctt ggacaaacgc tacgcccgtg cccagttgcc cgacggcaag ctgggtgagc
      241 tggtcgattt ggtgtccacc ataggctttg gcgacaaccc atccacggct cgtgatgtgc
      301 tgggccaagt ctacgaatac tttctgggca tgttcgccag cgccgagggc aagcgcggcg
      361 ggcagtttta caccccggcc agcatcgtca aaacactggt ggcgatcctc agcccccaca
      421 gcggccaggt gtatgacccc tgctgcggct cgggcggcat gtttgtgcag agcgaaaagt
      481 ttatcgaagc acacggcggc aagattggcg atgtgtccat ctacggccaa gaggccaacc
      541 ccaccacctg gcggctggca gccatgaacc tggccattcg cggcatcgac ttcaacctgg
      601 gccgcgaacc cggcgacacc ttcatccgta accagcaccc cgacctgcgg gccgacttca
      661 tcctggccaa cccacccttc aacatcagcg actggtggca cggcagcttg accggcgatg
      721 cccgctgggt ctatggcgat ccaccgcaag gcaatgccaa ctatgcttgg ctgcagcaca
      781 tgctgcacca cctcaagccc acaggccgcg caggcatcgt gctagccaat ggttccatga
      841 gcagcagcca aaacagcgag ggcaccatcc gcgccgccat ggtggacgcc gatgtggtgg
      901 aggtgatggt ggccctgccc ggccaactat tcttcaacac ccagataccg gcctgcctgt
      961 ggtttttggt caagcaaaag acgcaccgca agggccaagt actgttcatc gacgcccgca
     1021 aacaagccac catgatcagc cgggtgcaga gtgagttgac cgatgcggtg attgaacgca
     1081 ttgccgccac ggtggcggcg tggcgtggtg agccggatgc tggcggtgcc gccctgggtg
     1141 ctgataatca ctctgaaaat gatagctgct cccgcatgga atacgcggat attcccggtt
     1201 tttgccgatc cgtcccgctg gccgaaatcg cccagcacgg ccacgtcctc acccccggcc
     1261 gctacgttgg cgcggaagaa gtggaagaca acgacgaaga ctttgccacc aagatgcagc
     1321 agctcaccga gaagctgggt gagcagatgg ccaagggcgc ggagctggac cagttgatac
     1381 gcgccaagct gggagggttg gggtatgagt tctgaagtga cgatccacac agcagaatgc
     1441 cgccaatggc tgggtgagtt aaaaacccgt tttcgccagg ttcagctcaa ggccgccgtg
     1501 gcggtcaaca ccgccatgct ccagttctat tgggagttgg gggcggacat ggtggccaaa
     1561 caagcgcaat ttgcatgggg cagcggcttt ttgacccagc tcagcgccga tttgatgcgc
     1621 gaatttccgg atacaagggg cttttccaaa cgcaatttgg aactggttcg acagtggcac
     1681 ctgttttggc ttggtgcccc aattgggcag cagcctgttg cccaatttgc gaaacagcct
     1741 gtttcgcaaa ttctgtccat cccctggggc cacaacctgg ccattctggc caagtgcaag
     1801 cagcatgatg aagcgctgta ttacgcccaa gccacgcaaa cctatggctg gagccgcagc
     1861 gtgctggtgc accaaatcga gagcggcctg tggcagcgcg aaggcaaagc catcaccaac
     1921 tttgagcaaa cgctgcccgc cgctcagtcg gacctggcca cccaaatcct caaagacccg
     1981 tacaagtttg attttttatc gctcagcaaa gagcacaccg agcgcgaact ggaaacggac
     2041 ctgatcgccc acatcaccca atttctgctg gagctgggcg caggctttgc ctacattggg
     2101 cgccaagtgc cgctgcaagt gggagagcgc gaattctttt tggacctgct tttctatcac
     2161 acccgcctgc attgccatgt ggtggtggag ctgaaaaccg tggactttga acccgagttt
     2221 gcgggcaagc tcaacttcta catcaaggcc gtggacgaac agctgcgcgg tgagggcgac
     2281 caacccacca tcggtctgct gctgtgcaaa agcaaagacc gactggtggc cgaatacgcg
     2341 ctcagcgaca ttcaaaaacc catgggcctg tcgacctaca ccctgagcca caccctgccc
     2401 gaagccctgc gcgacaagct gcccagcatt gaggcgattg agcgggagtt ggggttggac
     2461 ggagccacgc catgaccgac aacctggatc aactcgccgg gcacatggtg gccctgttcc
     2521 gccaggcagt gccactcgcc aacgctgagg tggatgccat catctttaag atacgcgaac
     2581 aaggagaaac acaatgggcc tgaatgtcga acacctgttg cgcactgccg ccacgttaga
     2641 acaagccatg ctcgcgttgg gtaaaacacc aagcgcggat gaagtcatct acgacctgta
     2701 ccgcaacgct gcgataaaaa gttttgagtt gtcgctggaa actgccggaa agcttctgcg
     2761 caaagcgctc aaagcctttg gcgggaaccc gcgcagtgtc gatagcctgg tctttaatga
     2821 cgtgctgcgc cacagcggca aacacgccgt attggatcaa gcagaggtgg agcgttggtt
     2881 ggcctaccgt gccaaccgca acaacaccgc ccacgactac ggtgtgggtt ttgccaacga
     2941 cacactcacc ctgctccccg catacctggt agatgtgcgt gccttggccg aaaaattgca
     3001 gaaagtattt gatgccgcag cgtgaactca gcacgctgca actagcccca cagcatctac
     3061 agcttttgca agccctgctg cacacccatg tacctgaggc gcaggtgtgg gcctacggca
     3121 gccgtgttac cggtggtgcc cacgaaggca gcgacttgga tttggtgctg cgcaacccgg
     3181 cagacctgca acacgagtta gccaactggt tcgagctgaa agaagcactg caagacagtc
     3241 aattacccct gttggtagag ctgcacctgt ggtcgcacct gccgaaaacc tttcacccca
     3301 acattgaagc cgcttatgta gagctgcaag ctgggaggct tgacctgcca gaaacggatg
     3361 gaacctcccc atgaccgaca acctggacca actggccggg caaatggtga ccctgttccg
     3421 ccgggctgta cccctcgcta acgcagaagt ggatgccatc atccaaagcg gcgagcgtga
     3481 cataaatcgc atcgagcacc agctagacca catgctcggc ttttgctgcg ccccggacat
     3541 gctggtggtc tacaagcggc tgtgccggta ttacgacggc atcgatccgg tggctgcggc
     3601 ggggtacgtg caggcggttc gggagatgtg ggatatgccg gatgagatgg tgttgggggg
     3661 tggggtatga gtgcggagat tcggccaacc ctacttggcg atctgcttga gattacgtca
     3721 agtaaacgaa ttcacatggc ggattacgtt ccgtctggaa tacccttcta caggagcaaa
     3781 gaggtcattg agagagccaa gggcaacaac atctctaccg aactttttat ctcccataaa
     3841 cagtttgacc tcataaagca aaagttcggt agcccgcgag acggtgacat tctcctgacc
     3901 tcggtcggca cacttggcgt tccgtatgtc gtgcgcggcg atggggattt ctatttcaaa
     3961 gatggaaatc tcacgtggat gcggaatttc tcgaaagaaa tcagtccgca attcctttac
     4021 tactggctca cttcgccgca gattcaacaa aagctggacg aggtgtcaat tgggtcaacc
     4081 caaaaggcac taacaatttc cgcactcaag gcacttcaaa ttccactacc cacgcgggcg
     4141 actcaagatg gaattgtcga aatcttaggt gcaatttccg accgcatcac cctcctgcgc
     4201 gaaaccaatg ccacgctcga agccatcgcc caggcgctgt tcaagtcgtg gtttgtcgat
     4261 ttcgaccccg tgcgcgccaa gatggaaggc cgcgcccccg agggcatgga cgaggccacg
     4321 gcggcgctgt ttccggatgg gtttgaggag tcggagttgg ggttggtgcc gagggggtgg
     4381 caggtggcaa cgctcgcaaa tgcgttcgag ttgaatccat cgcgaaaact gccgaagggc
     4441 tctaagtcct tgtatcttga catggcgagt ctggcaacga gcgggcattg cgtggtggca
     4501 ccgatagacc gtgaattggg ttccggctcg aagttccaga atggcgatac gctgttggca
     4561 cgaatcact
//
LOCUS       DAOSLL010000393         4540 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16854_length_4540_cov_4.459755, whole genome shotgun sequence.
ACCESSION   DAOSLL010000393 DAOSLL010000000
VERSION     DAOSLL010000393.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16854_length_4540_cov_4.459755"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(231..356)
                     /locus_tag="PLB25_15415"
     CDS             complement(231..356)
                     /locus_tag="PLB25_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE42998.1"
                     /translation="MRSMTLAAGGSALDFVDLQRALFRLTTTLPGMSATPQHAIV"
     gene            526..711
                     /locus_tag="PLB25_15420"
     CDS             526..711
                     /locus_tag="PLB25_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018418772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HOE42999.1"
                     /translation="MKVSEMLKIIQNDGWKLVATRGSHRQFTHASKPGRVTLPGKPSD
                     DLAIGTQNSVFKQAGLK"
     gene            718..930
                     /locus_tag="PLB25_15425"
     CDS             718..930
                     /locus_tag="PLB25_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HOE43000.1"
                     /translation="MRYAVVIEKAVGNYSAYVPDLPGCIATGQTLGEIESEISEAITF
                     HIEGMIEDGLPVPQPASQVQYVEVAA"
     gene            973..1299
                     /locus_tag="PLB25_15430"
     CDS             973..1299
                     /locus_tag="PLB25_15430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43001.1"
                     /translation="MLSIIKRSNSSTPPSRLMVVGAWIGGIASAGGLVAMVLIAIDKV
                     SAGHGLDTYRTKWMVEDNWIGFLVFAFVTTVVVLAAAIIGWFQRRSEQRQVQQLQAKY
                     SEVNHG"
     gene            1399..1848
                     /locus_tag="PLB25_15435"
     CDS             1399..1848
                     /locus_tag="PLB25_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43002.1"
                     /translation="MDGSELRQRILAAFAPVPRPSRDEIAPHNCMECAELADDLAPYV
                     AADIPDESFQRHVWDMPLLSAEAKHYYFPAWLIRGLDDSEPWLSDAVSAVLNTLKNLE
                     HRWGPAQPYSREQWLVFQEWLSYVEKAGDAIDSDNIAEARARVGIEL"
     gene            1930..2256
                     /locus_tag="PLB25_15440"
     CDS             1930..2256
                     /locus_tag="PLB25_15440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43003.1"
                     /translation="MPIVAIYGFHGTFRGNAEYKYLHPALGASHKCMLFLAQPTDEQL
                     PEAALAECVKYGFAEVEFSGFGPLQVEVLNTPHFKGFAGFYQEALREGSALLFYPNCW
                     QSQPLW"
     gene            2417..2704
                     /locus_tag="PLB25_15445"
     CDS             2417..2704
                     /locus_tag="PLB25_15445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HOE43004.1"
                     /translation="MCRSIKTLFNFEPPATELEIRDASLQFVRKLSGFTVPSRANQAA
                     FDKAVEEVAATARKLIESLVTHAEPRNREDEAMKAKARSEERFGTKTGAKP"
     gene            2701..2970
                     /locus_tag="PLB25_15450"
     CDS             2701..2970
                     /locus_tag="PLB25_15450"
                     /inference="COORDINATES: protein motif:HMM:NF016855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HOE43005.1"
                     /translation="MTAQTIAERQGLGPKSQGMLAGAGINTITQLRRLGSVRAYAKVK
                     ATGANARLNLLWALEGALTGLPWQVVAREHRLSLLLALEALNGDA"
     gene            3081..3392
                     /locus_tag="PLB25_15455"
     CDS             3081..3392
                     /locus_tag="PLB25_15455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43006.1"
                     /translation="MTTTLDDVVAKAVALFGEDRAVQALALLDEYGTKSHEREVDRVK
                     LAILEVSDGKLNRLPYFVRCAKIDYRDVLTGARLGPMTEEEEAKWQASADCMLAQWNK
                     S"
     gene            3625..4263
                     /locus_tag="PLB25_15460"
     CDS             3625..4263
                     /locus_tag="PLB25_15460"
                     /inference="COORDINATES: protein motif:HMM:NF014816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HOE43007.1"
                     /translation="MTNTLYYSPGSCSLAPHIVLEEIGQPFSLSLVSTADGSTRKPEH
                     LRLNPKGRVPVLTNGDFVLTEAPAILLHLAIAHPRAGLLPSAADSLVRSIEWFNWLSG
                     TVHSVAIRQVWRPESFTLDQTQHDGIVAKGKENLESAFVLIEARMASRAWAVADRYSV
                     VDPFLLVFYRWGNRIGFDMPGSYKCWTQHTQRLLDCAAVGRALAQEGISVWQ"
BASE COUNT          971 a         1274 c         1280 g         1015 t
ORIGIN      
        1 ggggcagcac tttgcgtact acctggtggt gccggatgcc atctgtgagc gcccggccgt
       61 catggctttt cgcaactggc tgctggaaga gtcgcgcacg gaagaacccc gcagcggcgc
      121 tgtgacccca tagcggcctg gcccagcaaa ccctgcgctg tgctttggct tggtttggtt
      181 tgtataagaa atcggcctct agcgcttgca ggatatgcgc tgacagctat ttaaacaata
      241 gcgtgttgcg gcgttgctga catgccgggc agggtggtgg tcagcctgaa cagcgctctt
      301 tgcaggtcga caaagtccag cgccgagccc ccggctgcca acgtcattga cctcattgat
      361 gctcgccgag cagccattgc cgccaaaggt ggtcggagaa aagcgcgtgg acaacatgtt
      421 gttccagatc cgaccgccta cgggggcggc tgaacgccga tgtcctgtag tcagacagac
      481 cgacggtctt cggcaaatcc aacaagttgc tgctatcctt gacgaatgaa ggtcagcgaa
      541 atgctcaaga tcatccagaa cgatggatgg aaattggtcg caaccagggg tagccatcgg
      601 caatttacac atgcatccaa accagggcgt gtgacgcttc caggcaaacc cagtgacgat
      661 ttggctattg gcactcaaaa tagcgttttc aaacaagctg gtttgaagtg aggttttatg
      721 cgttacgcag tcgttatcga aaaagcggtt ggaaattatt cggcatatgt gccggatttg
      781 cctgggtgca tcgccaccgg tcagaccttg ggtgagatcg aatcagaaat cagcgaggcg
      841 atcacgtttc acatcgaagg aatgatcgaa gacggcttgc ctgtaccaca gccagcaagt
      901 caagttcagt acgttgaagt tgctgcatag caggtcattc catgggacgg cgacgcccca
      961 cgctgaattt cgatgttatc catcatcaag cgcagcaatt cttcaacccc gccatcgcga
     1021 ttgatggtcg tgggcgcgtg gattggcgga atcgcttccg cgggcggctt ggtcgcgatg
     1081 gtccttattg ccatcgacaa ggtcagtgcc gggcatggcc tcgacaccta tcgaaccaag
     1141 tggatggttg aagacaattg gatcggcttt cttgtctttg ccttcgtgac caccgtcgtg
     1201 gttttggctg ctgcaatcat tgggtggttt cagcggcgaa gcgagcagcg ccaagttcaa
     1261 cagcttcagg ccaagtattc cgaggtgaat catggatagc aggttgatcg attggatcac
     1321 caatggcggc ctatgccctc gccattcttc ggccagcagt gcggcgttgt cgtccaatca
     1381 tcgccaaggt tagacctcat ggacggatcc gagttgcggc aacgaatcct ggctgcgttt
     1441 gccccagtgc cccgtccatc acgtgatgaa attgcgccgc acaactgtat ggagtgcgcc
     1501 gaactagctg acgatcttgc gccttatgtg gcggcggaca tcccagacga atcatttcaa
     1561 aggcatgttt gggatatgcc gcttctgtca gcggaggcca aacactacta ttttcctgct
     1621 tggctcattc gtggccttga tgacagcgaa ccatggttgt ctgacgcggt cagcgccgtg
     1681 ctcaacactt tgaagaacct ggaacatcgc tggggccctg cgcaaccata ctcgcgcgag
     1741 cagtggcttg tttttcaaga atggctttcc tatgtagaga aggctggcga tgccatcgac
     1801 agcgacaaca tcgccgaagc acgggcgagg gtgggaattg agctctaact ttgcagtcga
     1861 cgcgcccgac cgtgcagtcg gctaccaccg gcctaccgtc agccggtgaa ttcaaacgtt
     1921 aaacctctta tgccaatcgt tgcaatttac ggttttcacg gcacctttcg tggcaatgcg
     1981 gaatataagt acctgcatcc tgcgcttggc gcctcacata agtgcatgtt gtttctcgcc
     2041 caaccaaccg atgagcaact gccagaagcg gcgcttgctg agtgcgtgaa gtacgggttt
     2101 gcggaggttg aattctctgg gtttggtccc ctgcaagtcg aggtcctcaa tacacctcac
     2161 ttcaaaggct tcgctgggtt ttaccaggag gctttgcgcg aagggagtgc gcttcttttt
     2221 tatccaaact gttggcagtc ccaaccattg tggtaatggc tattattggg caattcaaag
     2281 caaagtctgc gcccattgat gaaaccaacg tctggcatct tgattgacgc ggagacacaa
     2341 aagcaggaag ccgctttgca gcagttgccg tgcgccggtc atcgctaact ttaggcatcg
     2401 caaatacatc cctaccatgt gccgcagcat caagaccctc ttcaacttcg agcctcccgc
     2461 gaccgaattg gaaatccgtg acgcatcctt gcagttcgtc cgcaaactca gtggattcac
     2521 ggttccgtcc cgggcaaacc aggccgcctt cgacaaggca gtcgaggaag tggccgctac
     2581 ggcccgcaag ttgattgaga gtctggttac tcatgctgag ccgcgcaacc gagaggatga
     2641 ggccatgaag gccaaagctc gttccgaaga acgttttggt accaagactg gcgccaagcc
     2701 atgactgcac agaccatcgc ggaacgccag ggcctgggcc caaaatctca gggcatgctt
     2761 gcgggcgccg gcatcaacac gatcactcaa ctcaggcgac tcggttctgt gcgcgcgtac
     2821 gcaaaggtca aggcaactgg cgcgaacgca aggcttaatc tgctatgggc cttggaaggt
     2881 gcgctaaccg ggttaccgtg gcaggtggtc gcaagggagc ataggctcag cctgctactc
     2941 gccctcgagg cgctcaacgg cgatgcctga tccctcgctc aacttgacgc tccacggcat
     3001 ccctggcttg tggttcattt cattcttgcc ccagccagcc atgccgtttc gcgccggtta
     3061 cctccaacgt ttggcagcag atgacgacaa cccttgacga tgtagtggcg aaggccgttg
     3121 cgctgtttgg tgaagaccgc gcggtacaag cacttgctct gctcgacgaa tatggaacaa
     3181 agagccacga gcgcgaagtt gatcgcgtca aactggccat cctcgaagtc agtgacggga
     3241 agttgaaccg gcttccttac ttcgtgaggt gcgccaagat tgactatcga gatgtcctga
     3301 cgggggccag gctcgggccc atgactgaag aagaggaagc caagtggcaa gcctcggccg
     3361 attgcatgct tgcgcagtgg aacaagtcat agggcactgc ggtcggcgtt cgccattgac
     3421 tgttgacggc aggtctgctg cctggtttgc caataaactg cttgcccctt tgcttgagtc
     3481 gacctgctcc agcaagacaa gttgtccgcc cagtgcccag tccatcttgc tctggtcggc
     3541 cagtgggcct tgcctgagcg ttcggctcag ctcaaacgct agccgtcatg ctccagccat
     3601 cctgggctac tgccaaaatc accaatgacc aacacgcttt actactcacc cggatcgtgc
     3661 tcactcgccc cgcacattgt gttggaagag atcggccagc ctttttcact ctcactcgtc
     3721 tcaacggccg acggatcgac ccgtaagcca gagcatctcc gcttgaaccc caaaggtcgt
     3781 gtaccggtac tgaccaacgg tgattttgta ctgacagaag cgccagcgat tttgcttcac
     3841 ctcgccatcg cgcatccaag ggcaggcctg ctgccaagtg ctgccgacag ccttgtcagg
     3901 tcaatcgagt ggttcaactg gttgtctgga acagtgcact ccgttgcaat acgacaggtc
     3961 tggcggccgg aatcgttcac gctggatcaa acccaacatg acggtatcgt ggcaaagggc
     4021 aaagaaaacc tcgagtctgc ttttgtcctg atcgaggcgc ggatggctag ccgcgcttgg
     4081 gcagttgcgg atcgctactc ggtggtggac cccttccttt tggtgttcta ccgctggggc
     4141 aaccgtattg ggttcgacat gcctggaagt tacaagtgct ggacgcaaca tactcaacgt
     4201 ttacttgact gcgcagcggt tggacgggcg ctggcgcaag agggcatttc tgtctggcaa
     4261 taacgcacgg tgacgggatt ccatgccaca tgtccgatga cggctaactc ttccatcgac
     4321 ccgacacgcg gcggcgggca cagttgcgcg ccccgtcatg cgcgatgccg ccgctatgtg
     4381 ccgctgatgt caaacattga gccaacccgc cgacgtggtg ctcggttttg caaagcctgc
     4441 tgcagcccgg catctaattt gctttcattt cgatagctgg cagcgcagta tcaataagcg
     4501 ctggaggccg atttggcata taagacctgc ctggactacc
//
LOCUS       DAOSLL010000394         4532 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16894_length_4532_cov_4.196784, whole genome shotgun sequence.
ACCESSION   DAOSLL010000394 DAOSLL010000000
VERSION     DAOSLL010000394.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16894_length_4532_cov_4.196784"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..663)
                     /gene="leuD"
                     /locus_tag="PLB25_15465"
     CDS             complement(4..663)
                     /gene="leuD"
                     /locus_tag="PLB25_15465"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004626859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOE43008.1"
                     /translation="MKAFKQLNGLVCPLDRANVDTDAIIPKQFLKSIQRSGFGPYLFD
                     ELRYTNYGEPGMDCSTRPLNPDFVLNQPRYKGAQILLARDNFGCGSSREHAPWAIEDY
                     GLQVIIAPSFADIFFGNCYKNGILPLVASVEIVDRLFRECAATEGYRLNVELETQTVT
                     TPSGFSFEFDITPHRKHCLLHGLDEIGLTLQHADAIKNFEIKHLAAQPWLVFDTETTT
                     T"
     gene            complement(680..2089)
                     /gene="leuC"
                     /locus_tag="PLB25_15470"
     CDS             complement(680..2089)
                     /gene="leuC"
                     /locus_tag="PLB25_15470"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOE43009.1"
                     /translation="MAGKTLYDKLWESHLVHEEADGTCLIYIDRHLIHEVTSPQAFEG
                     MRLAGRTPWRSASIVANPDHNTPTRDWDKGIEEPIARLQVETLDANIKEFGALVYFPF
                     KHQNQGIIHVIGPEQGTTLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVLASQTL
                     IAKKSKRMQVRVEGQLSQGVTAKDVALAVIGKIGTAGGTGYAIEFAGAVIRSLSMEGR
                     MTVCNLAIEAGARAGLIAADATTTDYLKGRSFAPKGANWDLAVAYWNTLHSDLDAVFD
                     TVVEIDGSGIAPQVTWGTSPEQVLPVTARVPNPANEVDSVKRASLERALLYMGLSPDM
                     PIEQIKIDKVFIGSCTNSRIEDLRAAAKVIRGRQKASNVNQVLVVPGSGMVKRQAEQE
                     GLDQIFIAAGFEWREPGCSMCLAMNADRLPAGERCASTSNRNFEGRQGPGGRTHLVSP
                     EMAAAAAIAGHFVDIRTQL"
     gene            complement(2219..3796)
                     /gene="cimA"
                     /locus_tag="PLB25_15475"
     CDS             complement(2219..3796)
                     /gene="cimA"
                     /locus_tag="PLB25_15475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HOE43010.1"
                     /translation="MTKIQAYDSTLRDGAQAQGISYSIEDKLKIVQLLDQLGVSYIEA
                     GNPGSNPKDMEFFVRVAALELKNAKIIAFGSTRKVNVKVEEDDNLLSLLKAGVAAICI
                     FGKSWDYQVSHILSTSLTENLSMIGDTVGFLKRHHKEVVFDAEHFFDGYQSNPDYALQ
                     TLQAATDAGADHVCLCDTNGGTFPAEIYAITKLVASRTKVPVGIHCHNDGGMAVANSV
                     MAVQAGATQVQGTINGIGERCGNANLCAILPNLQLKLGLDCIPTAGMERLTTVARIVS
                     EIANIPFNEKAAYVGSDAFAHKGGMHVDAVNKNPKSYEHIDPERVGNSRRILMSEVAG
                     RSTVLAKIKEIDPTLTKDSPLTHKIINRLKELEYEGYQFETAESSFELVVRKILGKYQ
                     PIFELKDFKVIVNAPTVNGVNSFAMIKIRVGDQEEITAAEGDGPVHALDNAVRKALER
                     FYPLIREIKLTDYKVRVLDSDHASAAKVRVLIESTDHREKWTTIGVSTDIIDASWRAL
                     VDSIEYKLLRQQSIESA"
BASE COUNT          975 a         1226 c         1267 g         1064 t
ORIGIN      
        1 agattaagtt gttgtggtct cggtatcgaa aaccagccag ggctgtgccg ccaaatgttt
       61 gatttcaaag tttttgatgg catcggcgtg ttgcagcgtc agcccgattt cgtcaagacc
      121 atgaagcaag cagtgcttgc gatgcggcgt gatgtcaaat tcgaatgaga aacccgaagg
      181 cgttgtgacg gtctgggtct ccagttcaac gttgaggcga taaccctcgg tagccgcgca
      241 ttccctaaac agccggtcaa cgatttcgac tgaagcgacc aggggcaaga taccattttt
      301 gtagcaattg ccaaagaaaa tatcagcgaa tgacggcgca atgatgactt gcaagccgta
      361 gtcctcaatt gcccagggcg cgtgctcgcg gcttgagcca cagccaaaat tgtcgcgtgc
      421 cagcaaaatc tgggcacctt tgtagcgtgg ctggttgagt acaaagtccg ggttcaaggg
      481 tcgcgtgctg caatccatgc cgggctcacc gtaattggta tagcgcaatt cgtcaaacag
      541 ataggggcca aagcccgaac gctgaatcga cttgagaaac tgcttgggaa taatggcgtc
      601 ggtatcgaca ttggctcggt cgagtggaca caccagcccg ttaagttgct tgaatgcttt
      661 catggccgtc tttcgttgat tagagctggg ttcggatgtc gacaaaatgg ccagcgatgg
      721 cggcggcggc ggccatttcc gggctgacca ggtgggtgcg tccccccggc ccctggcggc
      781 cttcaaagtt acggttggag gttgaggcgc aacgctcgcc agcaggcaga cggtcggcat
      841 tcatggccag acacattgag cagcccggct cacgccactc aaaaccggca gcgataaata
      901 tctggtccaa tccctcctgc tcggcctgac gcttgaccat gccagaaccc ggcacgacca
      961 gcacctgatt gacattgctg gccttttgtc ggccccggat aaccttggcg gcggcgcgca
     1021 aatcttcgat gcgcgaattg gtacacgaac caatgaacac tttgtcaatt ttgatctgtt
     1081 cgatcggcat gtcggggctt agccccatat agagcaaggc gcgctcaagg ctggcgcgtt
     1141 taaccgaatc gacttcattg gccggatttg gaacacgcgc ggtgactggc aacacttgtt
     1201 cgggcgaagt gccccaagta acttgcggcg cgatcccgga gccatcgatt tcaaccaccg
     1261 tatcgaacac cgcatccagg tcggaatgca gggtgttcca gtaggccact gccaagtccc
     1321 aattagcccc cttgggggcg aagctgcgac ctttgaggta gtctgtagtg gttgcatcgg
     1381 cagcgatcag gccagcacgc gcaccggcct caatcgcaag attgcacacc gtcatgcgac
     1441 cttccatcga caggctgcga atcactgcgc cagcaaactc aatggcgtag ccggtaccgc
     1501 ccgcagtgcc gattttgccg atcacggcca gcgccacgtc tttggcggtt acgccttggc
     1561 tgagctggcc ttcgaccctg acttgcatcc gcttggattt tttggcaatc agtgtttgcg
     1621 aggccagcac atgctcgact tcggaggtgc cgataccgtg cgccagcgcg ccaaaggcgc
     1681 catgggtgga tgtgtgactg tcgccgcaga cgacggtcat gccaggcaag gtggtgcctt
     1741 gctctggacc gatgacgtga atgatgccct gattctggtg cttgaacggg aaatacacca
     1801 gcgcgccaaa ttccttgata ttggcatcca gggtctcaac ttgcaggcgg gcgataggct
     1861 cttcaatgcc cttgtcccag tccctggttg gcgtgttgtg gtcgggattg gcgacaatcg
     1921 aagcactgcg ccacggcgtg cgtccggcca ggcgcatgcc ctcaaaggct tgcggactgg
     1981 ttacctcgtg aatcaggtgg cggtcgatgt aaatcaggca agtgccatcg gcctcctcat
     2041 gcaccaagtg ggactcccat aatttgtcat aaagcgtttt accagccata gcgtgtcctt
     2101 aaataatctg gtttgaaaaa ggaatttagg cacccgattg gatgcttcaa gcgttgtctt
     2161 ggtggcataa cagctcttct ttgatagcgc cagccgtgag ccacttgctc gccgaatgtt
     2221 atgccgactc aatgctttgc tggcgcaata gtttgtactc gattgagtct actagggcgc
     2281 gccaagacgc gtcgatgatg tcggtggata cgccaatggt ggtccatttt tcgcggtgat
     2341 cggtggattc aatcaggacc cgcaccttgg cggcagaggc atggtcagaa tcaagcaccc
     2401 gaaccttgta atcggtcaac ttgatttccc gaatcaaagg ataaaagcgt tccagtgcct
     2461 tgcgtaccgc attgtcgagc gcatggactg ggccatctcc ttcagcggca gtgatttctt
     2521 cctgatcgcc aacacgaatc ttgatcatgg caaacgagtt gacaccattg actgtgggcg
     2581 cattgacaat gactttgaaa tcctttaact caaagatcgg ctggtatttg ccaagtatct
     2641 tgcgcaccac caactcaaag gagctttcgg cggtctcgaa ctggtagcct tcgtattcga
     2701 gttccttaag gcgattgatg atcttatgcg tcagtggcga gtctttggta agggttgggt
     2761 cgatttcctt gatttttgcc agcacggtac tgcgcccggc cacttcagac atcaggatgc
     2821 gccgactgtt gccgacccgc tcggggtcga tatgctcata cgacttgggg ttcttgttaa
     2881 ctgcgtcaac atgcatgccg cctttatggg caaaggcatc actgccgaca taggccgctt
     2941 tttcattgaa cgggatattc gcgatctcgc tgacaatgcg cgcgaccgtg gtcaagcgct
     3001 ccatacctgc cgtcggaata cagtccaaac caagttttaa ctgtaggttg ggcaagatgg
     3061 cgcacaaatt ggcgttgccg caccgctcgc caattccgtt gatggtgccc tgtacctgtg
     3121 tagcaccagc ctgcaccgcc atgaccgagt tggcgacagc catcccgcca tcgttatggc
     3181 aatgaatgcc aacaggaacc ttggtccggc tggcaaccag cttggtgatg gcgtaaatct
     3241 cggctggaaa agtgccgcca ttggtatcac acagacagac atggtcggca ccggcatcgg
     3301 tcgccgcctg caaggtttgc agcgcgtaat cggggttcga ctggtaacca tcaaagaagt
     3361 gttcggcatc gaaaactact tctttgtggt gccgtttgag aaagcctacg gtgtcgccga
     3421 tcatgctcag attttcagtc aggctggtgg acaaaatatg gctcacttgg tagtcccagc
     3481 ttttgccaaa tatgcaaatt gcagccaccc cggctttcag caaggacaaa aggttgtcgt
     3541 cttcctcgac tttgacattg accttgcgcg tgctgccaaa ggcgatgatc ttggcgttct
     3601 tgagttcgag ggccgcaacc cgcacaaaaa actccatgtc tttgggattc gatccggggt
     3661 tgcccgcctc gatataactg acaccaagtt gatcgagaag ctgaacaatt ttgagcttgt
     3721 cttcaatcga ataggaaatg ccttgagcct gcgctccgtc gcgcaatgta gagtcgtaag
     3781 cttgaatctt cgtcatggta aatacaggga ttttttatgg tgggcaaaag ttaattggca
     3841 gagcaaggcc ccaaccacac agtccggcaa gtatcgcact ccgaccgttc tgtgttgcgc
     3901 cgcctgccag cgcacccagt ccggccattg gcgaacgtga tgtcatggtc cgatttcgtg
     3961 tgctggattt gctgtgcttg caagccagtt gggcacatcg tgggctattg tccggatgct
     4021 tggcgtgaag ccgcttcgcc gtggcgcggg tggtatttgc atcggatttc agatcaatac
     4081 cgggcagcca tcaccagaaa gaggtgtgcc ctccctgtgc atgttcaacc ggactgtatc
     4141 gctcagggtc tgctgatcgt tgcaactgcg ctgcgatcca ccctcccagc atgttcagcc
     4201 agtcccacca ttgcgcctga cctgacgaaa atcatgccca aatatagctg ccatgcccgc
     4261 tcctgtgaaa tcgatcagat ggtcatggct accgggcagg aaaagtgcgc tctgtcccgg
     4321 tgatttgttg gtcagctcag cgaggttctg cgcaaaagcg tcgcggcaag ggtatcaatt
     4381 tgataatcga tgccaggaag cttatcacta tgggccattt gctcggttgc acgtcccggt
     4441 cacggccaat tgccgacctt gcgacagaat ctgcaggtgc cagcaggcaa accggcggca
     4501 ctggcgctgg catgattctt gtgagagcat cg
//
LOCUS       DAOSLL010000395         4509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16996_length_4509_cov_4.450157, whole genome shotgun sequence.
ACCESSION   DAOSLL010000395 DAOSLL010000000
VERSION     DAOSLL010000395.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16996_length_4509_cov_4.450157"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..935)
                     /locus_tag="PLB25_15480"
     CDS             complement(<1..935)
                     /locus_tag="PLB25_15480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HOE43011.1"
                     /translation="MSIESILSQVFGYPSFRGPQQDIIEHMVAGGDALVLMPTGGGKS
                     LCYQIPAIARQQAGHGITVVISPLIALMHDQVGALHEAGVQAEFLNSTLSWQAASRVE
                     QRLLQGDITLLYAAPERITTPRFLEQLDALHARGALSLFAIDEAHCVSQWGHDFRPEY
                     RALTVLHERYPGVPRIALTATADALTRADITRHLQLEEARLFISSFDRPNIRYTIVEK
                     RDATQQLLRFVRDFHLGDAGVVYCQSRKKVEDTAALLCDNGIAALPYHAGLDARVRQS
                     NQDQFLRMEGIVMVATIAFGMGIDKPDVRFVAHLD"
     gene            1470..1751
                     /locus_tag="PLB25_15485"
     CDS             1470..1751
                     /locus_tag="PLB25_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43012.1"
                     /translation="MLTFDDDHLLAVLAGKAQAAPAPTFAPEPVARSAPGKLCLPFAH
                     RWWRTMLAMVARKGDPDFFSVCSPLGSACSAKRAGWASALITRLLRRSA"
     gene            complement(1798..2931)
                     /locus_tag="PLB25_15490"
     CDS             complement(1798..2931)
                     /locus_tag="PLB25_15490"
                     /inference="COORDINATES: protein motif:HMM:NF014042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HOE43013.1"
                     /translation="MKQLVMFGGGSGSRDITMALCRQRYDITRVVPAWDSGGSSKALR
                     EHLSILAMGDIRQALMTLAHGEERVSSVVRFFNARLSEVATAADLRAEFDFYASGIHP
                     LLGTMKPDIRDAVLDYLRVFKARTGDDFDFRRGSIGNFVLTGAYLAHGRDINAAICVF
                     RKLCGIDGHVWPSTNHDGIELRADLRDGSLICGQDRVTALSPEQAQVGIRRVHLTTSD
                     TDAVVPVANPAVLAAIGTADAIVFGPGSFYTSTLAHLGVGGIAHAIAATRPEVPKILI
                     GNILECPETTGRTLAELVQAFDLALGPGILTHLFANRGWVPFTRVVKGYCYLLEGCMD
                     ASSGSLPQVFADDFEDPWSRGKHDASKVAATLGGILGRLGNES"
     gene            complement(3049..3345)
                     /locus_tag="PLB25_15495"
     CDS             complement(3049..3345)
                     /locus_tag="PLB25_15495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43014.1"
                     /translation="MEAWLKDRIPELIKSVLKQLRRHQIDAAADDFEDIACQVLPVEP
                     VSQSVNQADIMPSILRSVMIKSVMMTWNFTSTERIAKPIRYCDSSGQMQRCQAR"
     gene            complement(3604..4341)
                     /locus_tag="PLB25_15500"
     CDS             complement(3604..4341)
                     /locus_tag="PLB25_15500"
                     /EC_number="1.-.-.-"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF014573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008753 - NADPH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HOE43015.1"
                     /translation="MAKPFEQRRKSFSRCSLLKVTSEMNILIVFAHPEPKSFNGGLLD
                     TAKRTLESAGHTVVVSDLYQMGFNPVSDRHNFLVAKDPGYLKLQLEESHASAHGGFAP
                     ELEAEIRKIEACDLMIFQFPLWWFGLPAILKGWVDRTLAMGRTYGNGAVYGRGKFHGK
                     RALLSLTTGGPEEAFRKGGFNGDIDAILRPIQRGILEFIGFAVLKPQIHFGPVRASES
                     QRKRWLADYAKRLSTVAGENPINVGDY"
BASE COUNT          954 a         1320 c         1281 g          954 t
ORIGIN      
        1 ttgtccaggt gcgcaacaaa gcgcacgtcg ggcttgtcaa tacccatacc aaaggctatg
       61 gttgccacca tcacaatgcc ctccatgcgc aaaaactggt cctgattgga ctgacgcacc
      121 ctggcatcaa gcccggcgtg atagggcagc gcggcaatgc cgttgtcgca cagcagcgct
      181 gcagtgtctt caaccttttt gcgcgactgg caatacacca caccggcatc gcccaggtgg
      241 aagtcacgca caaagcgcag cagttgctgg gtggcgtcac gcttttcaac aatcgtgtag
      301 cggatattgg ggcggtcaaa gctgctgatg aacagcctgg cctcttcaag ctgcagatgt
      361 cgggtaatgt cagcgcgggt cagcgcatcg gccgtggcgg tgagcgcaat gcgtggcacg
      421 ccggggtacc gttcatgcag cacggtaagc gcccggtatt ccggccggaa gtcatgcccc
      481 cactggctga cgcaatgcgc ctcgtcaatg gcaaacagac tcaaagcccc gcgcgcatgc
      541 agcgcatcaa gttgctccag aaaacgcggc gtggtgatgc gttctggcgc ggcatacagc
      601 agtgtgatgt cgccctgcag cagccgttgc tcaacccttg atgcagcctg ccaggacagc
      661 gtcgagttca gaaactcggc ctgcacaccg gcttcatgca gcgcgccgac ctggtcgtgc
      721 atcagcgcaa tcagcggtga gatcaccacc gtgatgccgt gtccggcctg ctggcgggca
      781 atcgccggaa tctggtagca caggcttttg ccaccgccgg tgggcatcag caccagcgcg
      841 tcgccgcccg ccaccatgtg ctcgatgatg tcctgctgtg gtccgcgaaa cgaggggtaa
      901 ccaaaaacct ggctgagtat ggattcgata gacacttttg tttactatat ttatgatagc
      961 ttcctgcgct tatccagcaa gcgccaggag tcaatttgac ccattgcgct gcaaccggga
     1021 agcgaccgca gcggcccatt ttgcaccggc ttattgatgc ccaactgggc catgggtcag
     1081 caatgtcggt acagacgggt tcttgccggg gtcgattctc gccgttcgac ccataactcc
     1141 ggcaggcttg tggcgcacag tcgttggtcg aaacggtgac caccagtggc tggtttggca
     1201 aatgcctgag cttggccatt cgctggtcag caaccatgat cctggcttcc tcagttgatc
     1261 gcttgttctc atcaaaatgt ccctatgggt attgacttat tcagcttcta tgggggttac
     1321 ctattgggtc acagcgctac gcacccgatt gaaccgctga cggcgacttt gaaggccctc
     1381 gccgcgtctt tgacctgctg aaccattcct ttcgtggcca ccaaaaagag gcttgtttgc
     1441 tggatattgg cgaggctttc ttactgcccg tgctgacatt tgatgacgat cacctgctcg
     1501 cggttcttgc gggcaaggca caggcagcac cggcgcccac gtttgcgccc gagcctgttg
     1561 cgcgcagtgc gcccggcaag ctctgtctgc catttgccca ccgctggtgg cgcacgatgc
     1621 ttgccatggt tgcccgcaag ggtgacccgg actttttttc agtttgctca ccacttggct
     1681 cggcatgcag cgccaaaagg gctggctggg ccagcgcgct gattactcgc ctgctacggc
     1741 gttcggcttg aattcaattg aacacgcagg ttggacaatg acggcttgag ctggcggtca
     1801 gctttcgttg ccgagtctgc caagaatgcc acccagggtt gccgccacct tggatgcatc
     1861 gtgctttccc cggctccatg ggtcttcaaa gtcatcggca aaaacttgcg gtagcgaacc
     1921 gcttgacgcg tccatgcagc cctcaagcaa atagcagtat cccttgacaa cacgggtaaa
     1981 tggcacccag ccccggttgg caaaaagatg cgtcaagata ccgggcccga gcgccagatc
     2041 aaaggcctgc accaattcag ccagtgtccg gccggtggtt tcggggcact caagaatgtt
     2101 gccgatgaga atttttggca cctctggccg agtcgccgcg atcgcgtggg cgatgccacc
     2161 cacgccaaga tgcgccagcg tactggtgta gaaactgccc ggtccaaaca caattgcatc
     2221 ggccgtaccg atggccgcaa gcaccgccgg attggcgacc ggcacgacag catcggtgtc
     2281 cgaggtggtc aagtgcacgc gacgtatgcc aacctgggct tgttcggggc tcagggccgt
     2341 gacccgatcc tgcccgcaga tcagactgcc atcgcgcaaa tcagcgcgca gctcgatgcc
     2401 gtcgtggttg gtggatggcc aaacatggcc gtcgatgcca cagagtttgc ggaacacaca
     2461 aatcgcggca ttgatgtcgc ggccgtgcgc tagataggca ccggtcagca cgaagttgcc
     2521 gatgctgcca cgtcgaaagt cgaagtcatc gcctgttctg gccttgaaaa cccgcaggta
     2581 gtcaagcact gcgtcgcgaa tgtccggttt catggtcccg agcaagggat ggatgccgct
     2641 ggcgtagaag tcaaattcgg cccgcaaatc tgccgcagtc gcaacctcgg acaagcgcgc
     2701 gttgaaaaac ctgacaacgc tgctgacccg ctcctctcca tgggccaatg tcatcagggc
     2761 ctggcggatg tcgcccatcg ccaaaatgct caagtgctct cgtagcgcct tggaactgcc
     2821 accgctgtcc caggctggaa ccaccctggt gatgtcataa cgctgacgac acagcgccat
     2881 ggtgatgtcc cggctgccac tgcctccgcc aaacatgacg agttgcttca taaaagtcat
     2941 tctatggctt gcgcgtcagg tgtggctacg gatgacatca agtgctagcc caggcgccgg
     3001 atcggccaag gtcgtcgaat cgctgcaggc acaacagatc ctggcagcct atcgggcttg
     3061 gcaacgctgc atctgaccac tactatcaca atagcgaatt ggctttgcaa tacgttcggt
     3121 ggacgtgaaa ttccaagtca tcatgacgga cttgatcatt accgagcgca aaattgacgg
     3181 catgatgtct gcctgattga ctgactgact gactggttcc acaggaagca cttggcaagc
     3241 aatgtcttcg aaatcatcgg cagcagcatc aatttgatgt cgtctgagct gcttgagcac
     3301 acttttgatc agctcaggaa tgcggtcctt gagccatgcc tccatgcttt ttagagcacc
     3361 attgaagaag ccaaatttgg atcggcctat cgcgttatcg acccagtagt cgatggcgtc
     3421 gtggcgacgc tggccgggaa agcccggcga actgacttca gcaggttgcg ctcatcacag
     3481 cactgtgcgc ggcaaagccg ggggtgtacc ggtttttttg ggctgcggcg aactcggtca
     3541 cagttttgcc tgggtttgaa ccaagccgcc acaaacactt gaggcgatca gatgggcgat
     3601 gcctcagtag tcaccgacat tgatggggtt ctcaccggct acggtgctga ggcgcttagc
     3661 gtagtcggca agccatctct tgcgctggga ttcgctcgcc cgaaccgggc caaagtgaat
     3721 ttgaggcttc agcactgcaa agccgataaa ttccagaatg ccgcgctgta tgggtcgcag
     3781 gatagcgtcg atgtcgccat tgaagccgcc tttgcgaaaa gcctcttccg ggccaccagt
     3841 ggttagtgaa agcaaggcac gcttgccgtg gaattttccc ctgccgtaaa ccgccccgtt
     3901 cccgtaagtg cggcccattg ccagggttcg atctacccac cctttgagta ttgccggcaa
     3961 cccaaaccac cacagcggga actgaaagat catcaaatca cacgcctcga tcttgcgaat
     4021 ctcggcttcg agttcaggtg caaaaccgcc gtgtgcgctt gcatggcttt cctcaagctg
     4081 cagcttgagg taaccggggt cctttgccac cagaaagtta tgacgatccg aaaccggatt
     4141 gaaacccatt tgatagagat cgctgacaac gacggtatgc cccgccgatt caagggtacg
     4201 tttggccgtg tccaacaagc caccgttaaa gctcttcggt tccgggtgag caaagacaat
     4261 aagaatgttc atttcggatg tgaccttcaa aagtgagcag cgcgaaaagc ttttgcggcg
     4321 ctgctcaaag ggtttagcca agcaataagt ttgcctgact catggcaacg gtcgcgagaa
     4381 tcgagcccca acaggccagt ttgtgccgga tttccaggcg gatagcccaa ctgttgcgca
     4441 aaagaccggt acgacacgag ccgtcgcggt cggttcgcag ccgatgcgcc agaagtccaa
     4501 ccggttgct
//
LOCUS       DAOSLL010000396         4509 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_16999_length_4509_cov_4.107095, whole genome shotgun sequence.
ACCESSION   DAOSLL010000396 DAOSLL010000000
VERSION     DAOSLL010000396.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4509
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16999_length_4509_cov_4.107095"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="PLB25_15505"
     CDS             <1..599
                     /locus_tag="PLB25_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE43016.1"
                     /translation="GNPLRAAFLNLPGPEQRCAGRSGPLRLLVVGGSLGARALNEIVP
                     QALALLPPEQRPVVLHQSGANQIDALRANYRAAGVTAELTPFIDNTAQAFADADLVIC
                     RAGASTVTELAAVGVAALFVPFPSAVDDHQTTNARFLVDQDAGWLVRQSEMTPRDLAD
                     MLLKMERDTLMDKGLRAKKMQKTGATEQIVQVCEEAAK"
     gene            877..2310
                     /gene="murC"
                     /locus_tag="PLB25_15510"
     CDS             877..2310
                     /gene="murC"
                     /locus_tag="PLB25_15510"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HOE43017.1"
                     /translation="MKHAIKHIHFVGIGGVGMSGIAEILFNLGYTISGSDQVDSATLQ
                     RLASLGIQTYIGHAAVQVEGADAVVTSTAVQASNPEVQAARDKRIPVVPRALMLAELM
                     RLKTGIAIAGTHGKTTTTSLVASVLAEAGLDPTFVIGGRLNSAGANARLGQGDYIVVE
                     ADESDASFLNLLPVMAVVTNIDADHMETYGHDFGRLQQAFVDFLHRMPFYGTAILCTD
                     DPAVRAIVPQVTCPITSYGFDEGAEVRALNVRAVAGQMHFTVQRRNGLVLPDLAVVLN
                     LPGRHNVLNALSAIAIAVELNIADDAVLRALAEFKGVGRRFQRYGDLPGCNGGTFALV
                     DDYGHHPAEMAATLAAARGAFPGRRLLLAFQPHRFTRTRDCFDDFVKVIAQADAVLLA
                     EVYAAGEAPIVAADGRSLARALRVAGRIEPVFVDDINNMAQTIVMNAQDGDVVLCMGA
                     GSIGAVSAKVVQMLQNAELSAQEGQGL"
     gene            2307..3266
                     /locus_tag="PLB25_15515"
     CDS             2307..3266
                     /locus_tag="PLB25_15515"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HOE43018.1"
                     /translation="MSQFDPKKEKVAVLLGGRSAERAVSLMSGQGVLQALLSQGIDAQ
                     AFDPCERDLGLLKAEGFTRCFIALHGRFGEDGTVQGALELLGIPYTGPGVMASSMAID
                     KIMTKRLWRADGLPTPAWQMVNSAESAQRAFAELAGAGQPMIIKPAREGSTLGLSKVT
                     TVDQCASAYALAAGLDDLVLCEQFIAGDEVTVPVLGSGADARALPVIRIEAPDGNYDY
                     QNKYFTDTTRYLVPCGLPDGEEAAIQQLVLQAFHSLGCRGWSRADVMIDAQTRQPYLL
                     EINTSPGMTGHSLVPMSAKAAGISYADLCLRILQLATLDYPRA"
     gene            3263..4075
                     /locus_tag="PLB25_15520"
     CDS             3263..4075
                     /locus_tag="PLB25_15520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HOE43019.1"
                     /translation="MNTTAPAVLPFDVRLMNAVASALFAAALLMLLGALLLWVARLPL
                     FAIQGVSVSGDVAHYNAITLRANVMPRLQGTFFTLDLKEARKVFESVPWVRRAVVRRE
                     FPNRLKVQLQEHQAVAFWGAEGESRLVNSFGEVFEANVGEIEQDNLPRLNGPEGQSAQ
                     VLAMHQTLQPLFTPMDLTLDQLELTARGGWRAQLDTGTELELGSGETAELMARAERFL
                     KTVAQVTARYQRNPSQLASVDLRHSDGYAIRMTGVSTLAGDEMKKTATQDNR"
     gene            4080..>4509
                     /locus_tag="PLB25_15525"
     CDS             4080..>4509
                     /locus_tag="PLB25_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HOE43020.1"
                     /translation="MAKEYKDLIVGLDIGTAKVMVVVAEVMPGGELKLAGLGVAPSNG
                     LKRGVVVNIDATVQSIQQALREAELMADCKITRVYTGITGSHIRGINSHGMVAIKDRE
                     VTAADVARVVETAKAINISTDQRLLLVEPQEFIIDGQDVKE"
BASE COUNT          847 a         1303 c         1470 g          889 t
ORIGIN      
        1 ttggcaaccc gttgcgcgcc gcgtttttga acctgcccgg gccagagcag cgctgtgccg
       61 ggcgcagcgg gccgctgcgt ttgctggtgg tgggtggtag cctgggggcc agggcgctca
      121 acgagatcgt gccgcaggca ttggcgctgc tgcctcctga gcagcgcccg gtggtgctgc
      181 accagagtgg ggccaatcag atcgatgcct tgcgcgccaa ctaccgggcc gccggggtga
      241 cagcagaatt gacaccgttc attgacaaca cggcgcaggc ttttgccgat gccgacctgg
      301 tgatttgccg cgccggtgcc agcaccgtga ccgaacttgc ggcggtgggt gtggcagcgc
      361 tgtttgtgcc gtttccatcg gcggtcgatg accatcagac caccaatgcg cggtttttgg
      421 tcgatcagga tgccggctgg ctggtgcgcc aatctgaaat gacgcctcgg gatttggccg
      481 atatgctgct aaaaatggag cgtgatacgc tgatggataa agggttgcgg gccaaaaaga
      541 tgcaaaaaac cggtgcgaca gaacagatcg tgcaggtttg tgaggaggcg gcgaaatgaa
      601 tgattgtgtt gcggcgcaag cggggttagc ccctacccta tccccccgcc ctttcccgcc
      661 ccgccgggct agaaagggag ccaactgccg tatgtggccg gtcgtcttgc cgagcgacag
      721 cggtgcgcca agcgtggtcg atggcttggt gatggttggt gatcaaacgg attggttagt
      781 ggctggaggc caatcccaaa ctgaccgaac agttgcaggt tcggaagtta attcgactct
      841 gaccccaatt gttccaattg tttgcgaagt tccagcatga aacacgccat caaacacatc
      901 cattttgtcg gcattggcgg ggtaggcatg tccggcattg ccgaaatatt gttcaacctg
      961 ggctacacca tctccggctc ggatcaggtt gacagtgcca ccttgcaacg gctggcctcg
     1021 ctgggcatcc agacctacat tggtcacgcc gccgtccagg tcgaaggagc cgacgcggtg
     1081 gtcacctcaa ctgcggtgca ggccagcaac cccgaggtac aggcggcgcg cgacaagcgc
     1141 atcccggtgg tgccacgggc cctgatgctg gccgaactga tgcgcctgaa aaccggcatt
     1201 gcgattgctg gcacccacgg caagaccacc accaccagtc tggtcgccag tgtgctggcc
     1261 gaggccggtc tggacccgac atttgtgatt ggcgggcgtc tcaacagcgc cggtgccaac
     1321 gcccggctgg ggcaaggcga ctacatcgtg gtcgaggcag acgaatccga tgcctcgttt
     1381 ttgaacctgc tgccggtgat ggcggtggtc accaacatcg atgccgacca catggaaacc
     1441 tatgggcatg attttggtcg gctccagcag gcatttgtcg attttctgca ccgcatgccg
     1501 ttttatggca cagcgatctt gtgcactgac gacccagcgg tgcgcgccat cgtgccgcag
     1561 gtgacttgtc cgatcaccag ctacggcttt gacgagggtg ccgaggtacg cgcgctcaat
     1621 gtacgggcgg ttgcaggcca gatgcatttc acggtgcagc ggcgtaatgg cctggtgctg
     1681 cccgatctgg ctgtggtgct gaacctgccg gggcggcaca acgtactcaa cgcgctgtcg
     1741 gcgattgcga ttgcagtcga actcaatatt gccgacgatg cggtgttgcg ggccctggct
     1801 gaattcaagg gtgtcgggcg gcgctttcag cgttacggtg atttgcctgg ttgcaacggt
     1861 ggcacctttg ccctggttga tgactacggt caccatccgg ccgagatggc tgccacgctg
     1921 gccgctgcac gcggtgcgtt tcccggcaga cgactgctgc tggcgtttca gccacatcgt
     1981 tttacccgga cccgcgactg ttttgatgac tttgtcaagg tcatcgcgca agctgatgcg
     2041 gtgttgctgg ccgaggtcta tgcggccggc gaggcaccga tcgtggcggc cgatggccgc
     2101 agtctggcgc gcgcgctgcg ggtggccggg cgcatcgagc cagtgtttgt cgatgacatc
     2161 aacaacatgg cgcaaaccat cgtgatgaat gcccaggacg gtgacgtggt gctgtgcatg
     2221 ggtgccggtt cgatcggcgc ggtgagcgcc aaagttgtcc aaatgctaca aaatgcagag
     2281 ctatcagcgc aggagggaca agggctatga gccagtttga tccaaaaaaa gagaaggtcg
     2341 cagtgctcct gggtggccgc tctgcggagc gcgccgtctc gctgatgtcg ggccaggggg
     2401 tgctgcaggc cttgctatcg cagggcatag atgcgcaagc gtttgacccg tgcgagcgtg
     2461 acctcggcct gctcaaagcc gagggcttta cccgctgctt cattgccctg catggccgct
     2521 ttggcgaaga cggcacagtg caaggcgcgc ttgaactgct cggcattccc tacaccggcc
     2581 ctggcgtgat ggcatcgagc atggcgattg acaaaatcat gaccaagcga ctatggcgcg
     2641 ccgatggcct gccgacaccc gcctggcaaa tggtcaacag cgccgagtct gcgcagcggg
     2701 cctttgccga gctcgctggc gctggccagc cgatgatcat caaacccgcc cgcgaaggct
     2761 ccaccctggg cctgagcaag gtcacaacgg ttgaccaatg tgcgtcggcc tatgccctgg
     2821 ccgccggtct ggacgatctg gtgctgtgcg aacagttcat tgccggtgac gaggtgaccg
     2881 tgccggtgct gggcagcggt gccgatgcgc gagccctgcc ggtgatccgg atcgaagcgc
     2941 ctgacggcaa ctacgactac cagaacaagt acttcaccga caccacccgc tatctggtgc
     3001 cgtgcggttt gccggacggc gaagaggcag cgattcaaca actggtgctg caagccttcc
     3061 acagtctggg ctgccgtggc tggtcgcgcg ccgacgtgat gattgatgcc cagacccgcc
     3121 agccgtattt gctggaaatc aacaccagtc cgggcatgac cgggcattcg ctggtgccga
     3181 tgtcggccaa agccgccggt atcagctatg ctgacctgtg cctgcgcatc ttgcagcttg
     3241 ccactttgga ctacccccgc gcatgaatac gactgcgcct gccgtgctac cgttcgatgt
     3301 tcgattgatg aacgcggtgg ccagcgcgct gtttgctgct gcgctgctga tgctgctggg
     3361 cgcactgctg ctttgggttg cacgcttgcc actgtttgcc attcaggggg tcagcgtcag
     3421 cggcgatgtg gcgcattaca acgccatcac cctgcgcgcc aatgtgatgc cgcgcctgca
     3481 gggcaccttt ttcacgctgg atctgaaaga agcgcgcaag gtgtttgagt cggtgccctg
     3541 ggttcggcgg gcggtggtgc ggcgcgaatt tcccaaccgg ttgaaggtac agttgcagga
     3601 acaccaggcg gtggcgtttt ggggtgcaga gggtgaatca aggttggtca acagttttgg
     3661 tgaagtgttt gaggccaatg ttggcgagat cgaacaggac aacctgccgc gcctgaacgg
     3721 gccggagggc cagtcagccc aggtgttggc gatgcaccag accttgcagc ccttgttcac
     3781 accgatggac ctgacgctgg accaactcga actcaccgcg cgcggcggct ggcgggctca
     3841 gcttgacact ggcaccgagc tggagctcgg tagcggcgag acggctgagt tgatggcacg
     3901 tgccgagcgc tttctgaaaa ccgtggcgca ggtgacagct cgttaccagc gcaacccaag
     3961 ccaactggca tcggtcgatt tgcgccactc ggatggttat gcgatccgca tgactggcgt
     4021 gagcacactg gcaggtgacg agatgaaaaa aacagcaacc caagacaaca ggtgacttca
     4081 tggcaaaaga atacaaggat ttgattgtcg gtctggacat cggcaccgcc aaggtgatgg
     4141 tggtggtggc cgaggtcatg cctggtggcg agctcaagct ggccggcctg ggcgtggccc
     4201 ccagcaatgg cttgaaacgc ggtgtggtgg tcaacattga tgccaccgtg caaagcattc
     4261 agcaggcgct gcgcgaagcc gagttgatgg ccgactgcaa gatcacccgg gtctacaccg
     4321 gcatcaccgg cagccacatc cggggtatca attcgcacgg catggtggcc atcaaggacc
     4381 gcgaagtcac ggctgctgac gtggcccgtg tggtggaaac cgccaaggcc atcaacatct
     4441 ccaccgacca gcgcctgctg ctggtcgagc cgcaggaatt catcatcgat ggccaggacg
     4501 tcaaggaac
//
LOCUS       DAOSLL010000397         4507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17014_length_4507_cov_4.805256, whole genome shotgun sequence.
ACCESSION   DAOSLL010000397 DAOSLL010000000
VERSION     DAOSLL010000397.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17014_length_4507_cov_4.805256"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="PLB25_15530"
     CDS             <1..247
                     /locus_tag="PLB25_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43021.1"
                     /translation="LPGKPWGDFQHARRLAIASGSTFAPGGDNRFATAPISLKEDFGL
                     NEALLGIFSGIRETGITGRRRTARSESLAASPDISGR"
     gene            381..983
                     /locus_tag="PLB25_15535"
     CDS             381..983
                     /locus_tag="PLB25_15535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017450163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HOE43022.1"
                     /translation="MKLGIRGLTLAVGIIAAVTSQAQTPAFTLSSSDLASGTFANKFV
                     LNGFGCKGDNVSPALQWSNVPPGTKSLALQVYDPDAPTGSGFWHWAVYNIPASVSGLA
                     QGAGNGATALPPGAFGGNTDFMDTGATGGNGNYGGPCPPTGDKPHRYIFTLFALAVPQ
                     LEVAGGVPKTGTAGLFGFVLNKGVGPALLGKASFTATYGR"
     gene            1825..2655
                     /locus_tag="PLB25_15540"
     CDS             1825..2655
                     /locus_tag="PLB25_15540"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOE43023.1"
                     /translation="MKPRIFFASLAAFLLIPTAWAGFVAFESAGANPSDVTLSRDAFR
                     TAVGGGTVSGANGSFGGLRREINWDGVPTQFSDPNNLPADFFNVNSPRGVVLSTPGTG
                     FLVSANAGSATPALFGFPSDFQTFSSQRLFTAVGSNITDVLFYLPGTTTAATTSAFAA
                     IFVDVEVAGATKIEFFDQNDLMIFSRSALVAGNQGLSFVGAVADAGERISRVRLTSGL
                     NTIIANGVLGNLNDDVVVMDDFLYAEPLQSVPEPASIVLVTVGLVGCLAKLRRRRGRV
                     "
     gene            complement(3154..3819)
                     /locus_tag="PLB25_15545"
     CDS             complement(3154..3819)
                     /locus_tag="PLB25_15545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43024.1"
                     /translation="MNKEKVSLLIAIGLVPLLAGCVNQKLAANTQQNAPTTQVLLIRL
                     HPEDLRTGGLSIITPSSATGQEEDRQALALAFTEVLLKVRPDLRIVPLPQTLSAINRT
                     GLTREYRQMFEDYRLAGIFDNEALQKVARVTGTRYVAQLKLGTFRQESKDRFGMLGLR
                     VLQTKTSTIRLFLQIWDSTDGSVAWEGAQESTLSHESLAEEYVSMKSIVQESARDLLA
                     RLP"
     gene            complement(4141..4350)
                     /locus_tag="PLB25_15550"
     CDS             complement(4141..4350)
                     /locus_tag="PLB25_15550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43025.1"
                     /translation="MKTSTASDAPAFIVGSPQEPVAAVEPLKKFALIGKGLHGEDRPV
                     GFYTTATRSHPGGLTELFGAVSGAY"
BASE COUNT          913 a         1273 c         1279 g         1042 t
ORIGIN      
        1 gctgcccggc aaaccctggg gtgacttcca gcatgcccgg cgactggcca tagccagcgg
       61 ctcgacattc gcaccgggcg gtgacaacag atttgccact gcgccaatct cgctcaaaga
      121 agacttcggc ctgaatgagg cactgttggg gattttttca ggcatccggg aaactgggat
      181 taccggccgc cgtcgcactg cgcgcagtga aagcctggcg gccagtcctg acatcagcgg
      241 ccgctgaagc gcgccaggta ttgggactct gccaccaaca ctgctgcgaa agatagggta
      301 agtttccttt ctcgcgttta ggatttcagc tagcatttgc ggcgctggtt tggtcagaaa
      361 gcacacttta aggagatgcc atgaaacttg gaatacgggg cctgacattg gcggttggca
      421 ttattgcagc cgtcacttcc caggcccaaa caccggcctt tacgctgtcc agtagcgacc
      481 tggcaagcgg cacattcgcc aacaagtttg tgttgaacgg atttggctgc aagggtgaca
      541 acgtttcacc tgccttgcaa tggtcaaacg tcccgcctgg cacgaaatcg ctggcactgc
      601 aggtttacga cccggatgcg cctacgggca gcgggttctg gcactgggcg gtctacaaca
      661 ttcccgcaag cgtgtccggt ttggcgcagg gagcgggaaa cggcgctacg gccttgccgc
      721 caggcgcatt tgggggaaat actgacttta tggatactgg cgcaacgggt ggcaacggca
      781 attacggtgg accctgcccg ccgactggag acaagccaca ccgctacata ttcaccttgt
      841 ttgcgctggc ggtcccgcaa ttggaggtgg ctggcggtgt tccaaagacc ggcacggcag
      901 gcttgtttgg gttcgtgctg aacaaagggg ttggccctgc gctcttgggc aaggccagct
      961 tcactgccac ctacgggcgc taggcgaaac tgctcggggc atctcagcca cccaggcata
     1021 gcgattttgg ggcgcatcag gggggccgct actttttaca gttcagccat tgctctatcg
     1081 cagttcagct ttggaaaatt ggctgacaga gcccgatcac tctttcagaa gctgccctct
     1141 aacctcgatg gggagtttcc gcgccgcttg ctgttttgcg ccatgggtgc gctctgaagc
     1201 aacggtgtac cggtatcgga cagtgtgctg gcgaacagtt gtatgcaacg caaagcgcta
     1261 tatttggctt gggccttcca aaagcctctg tgctacttca ccccacagca cagctcgcaa
     1321 gagatttaac ccagccttta cccgctgggt tcttttttgt cgggcagggc tcgtttggta
     1381 cctgctgcaa ccgtctgagc ggacggcctg ctccaacacg gttgatctgc cgccgttcgg
     1441 acccggctca attctggcgg gcactcgaca aaagagcgcc gccatcggct ttgtatgcgg
     1501 cgctttccgg tgccgcagta gcgacatggg gcctgccgaa gaggcccaaa cccccccaat
     1561 aactgtcgaa aaacgttaca tggtgggcga aacatcgtcc caagaccagc gccacctgga
     1621 aaaatcttca atcaatacag tgtgttacga gtattttctg agtttggctc aggtattgct
     1681 ttggatgccg aatgaccagc atgcggttaa gtgttgggca tacctacgtg tctgattctg
     1741 ttttatctct gccctcatat ccacctgcaa tgagatgaaa acgggattgg tcattttttc
     1801 aagttgcatg aatcaggagg ttccgtgaaa ccacgcatat tttttgcatc ccttgccgcg
     1861 tttcttttga ttcctacggc ttgggctggc tttgtcgctt tcgagtcggc tggtgccaac
     1921 cccagtgacg tcaccttgtc gcgagatgcc tttcgtacgg ccgtcggtgg cggaacggtc
     1981 tcaggtgcca atggctcgtt cggcggcctt cgccgagaga tcaactggga cggtgtacct
     2041 acccaattct ccgacccgaa taacttgcct gccgatttct tcaacgtcaa ttctccccga
     2101 ggggttgtat tgagcacgcc aggaacgggc ttcctcgtga gcgccaacgc cgggtcggcc
     2161 acgcccgcgc tgttcggttt tcccagcgac tttcagacat tcagctcgca gcggcttttc
     2221 actgctgtcg gcagcaacat cacggatgtt ctgttctacc tgccaggtac gacgacggct
     2281 gccaccacca gtgcctttgc ggcgatcttc gttgatgtag aagtagccgg tgcgacgaag
     2341 atcgagttct tcgatcagaa cgatttgatg attttctccc gaagtgcttt agtcgccggg
     2401 aaccagggat tgagcttcgt cggtgcggtt gccgatgccg gagaaagaat cagccgggtt
     2461 cgcctcactt cgggcctgaa tacgattatt gccaatggcg tgctgggcaa cctgaatgac
     2521 gacgttgtgg tgatggacga ttttctgtat gccgagccac tgcaaagcgt ccccgagcca
     2581 gcaagcattg tgctggttac cgttgggctg gttggctgtc ttgcaaagtt gcgccgccgc
     2641 cggggccgtg tctgatcaag acaatcaaac ggtgcaccac gcgggtatac ggtgcaagtg
     2701 acacgtctgc atcgtattgg gccaaacgca atgcctcctg ttgctgccaa ctgaaaactg
     2761 aactgcattt ggagcgtatg ccgacccagc attgactccg gtgtttacca aaccggccga
     2821 cttttcaggc cggagccatg caagtgatca ccatggaaat gcgtgcatgc ccgctacaac
     2881 gcgccgttca cgccatggtt tttgcggcga gatgaaattt gggtgcatct ggccaaactg
     2941 cgatgagcgg acatgacgcg atctgatgtc cgcagcagcg caatatcggc aaaacactgc
     3001 tgccgctagc cgcagctcca cctgtggcag actgacaagc gcgcggtccg aaccgctttc
     3061 gctgacacga acgtccagcc tgtgagccaa gcaggccagg agcggctggc gagcaaatgc
     3121 aaccgtgccc ggggggccac ccttcctcat aacctatggc aatcgtgcca ggaggtcgcg
     3181 tgctgactct tggacaatgc tcttcatcga cacatattcc tccgccagcg attcgtgcga
     3241 aagcgttgat tcctgtgcgc cttcccatgc caccgagccg tcggtgctgt cccatatttg
     3301 gaggaacagc cggatagtgc tggttttggt ttgcagcacc cgcaagccca acatgccaaa
     3361 ccggtctttg gactcttgcc ggaacgttcc gagtttgagc tgcgccacat aacgcgttcc
     3421 ggttacccgt gccactttct ggagcgcctc gttgtcgaat atgccagcca agcgatagtc
     3481 ttcgaacatc tgcctgtatt cgcgcgtcaa tcccgtgcga ttgatcgcac tgagggtttg
     3541 cgggagcggc acgatacgca ggtccgggcg aaccttcagc agtacttcgg taaaggcgag
     3601 tgccaacgct tgcctgtctt cttcctgccc ggttgccgaa gatggcgtaa tgattgagag
     3661 accccctgtt cggaggtcct cggggtgcag tcgaatcaac agcacctgcg tcgttggtgc
     3721 gttttgctgg gtgtttgcgg ccagtttttg gttgacgcaa ccggccagca acggcacaag
     3781 tcctatggct atcagaagcg ataccttttc tttgttcatg atcgatttcc tcggtgactc
     3841 atgctagaaa gtcggcagaa cattctctgt gcgcttgcac acaaacgctt cgcgtcaacc
     3901 caggcaatga tgaaacaacc tgagccggta gcgaagttgc tgacgagtgt tgaactcatt
     3961 gcgtttccag accaatgcga cattcggcac gaagccgcag acagtgctaa gccaccgcag
     4021 ggcgaagcag caaactgtcc gactggtatg aaaatggaat cagatggaca taccggccct
     4081 cgcaacccgg caattcaggt gccgtgccaa ctggtggcag gaaaagtaca gcggcgactg
     4141 tcaatatgcc ccagatactg ccccaaaaag ctccgtcagc ccgccaggat gcgatcttgt
     4201 cgccgttgtg taaaacccaa ccgggcgatc ctctccgtga aggcccttgc cgatcaaggc
     4261 aaatttcttg agcggctcaa cggcggcaac tggttcctga ggggaaccga caatgaatgc
     4321 gggcgcatcg gatgcagtac tggttttcat gtcaaatctt tgaatagtgg ttaagcggtt
     4381 gatggcatgg gcaacccggt acgtccctgc cacggattgc gcgctggtat tgccgcagtt
     4441 gatcgtcagt tttcaccgtt gcagtccttg gtttgcttgc tgcctggtca ctgtcgttct
     4501 tttcgat
//
LOCUS       DAOSLL010000398         4494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17092_length_4494_cov_3.959676, whole genome shotgun sequence.
ACCESSION   DAOSLL010000398 DAOSLL010000000
VERSION     DAOSLL010000398.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17092_length_4494_cov_3.959676"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            153..395
                     /locus_tag="PLB25_15555"
                     /pseudo
     CDS             153..395
                     /locus_tag="PLB25_15555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593312.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
     gene            621..962
                     /locus_tag="PLB25_15560"
     CDS             621..962
                     /locus_tag="PLB25_15560"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOE43026.1"
                     /translation="MHGRSSRISAQRVDTCYELMDAAYCSTVIREDIQAAGRVPLIDH
                     NPRKGEKKEFAPDEAERYKIRSTAERCNARLKAEFGARHVQVRGHAKVMCHLMLRVVA
                     LCADQFTRLLM"
     gene            complement(1101..1988)
                     /gene="cobA"
                     /locus_tag="PLB25_15565"
     CDS             complement(1101..1988)
                     /gene="cobA"
                     /locus_tag="PLB25_15565"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019042733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HOE43027.1"
                     /translation="MTSFATSLTLVPRPGPSGKCTLVGAGPGDPELLTLKALKAIQAA
                     SVLLADDLISEEILALASPKARIIRVGKRGGCKSTPQAFIEKLILLAVREGENVVRLK
                     GGDPFIFGRGGEEVARLQAAGVVVSVVNGITAGLAAASSLGVPLTHRDQAQGVVFVTG
                     HTKPGGLGIDWPRLASMANHLRLTLVIYMGVRGAAQIEQELLHGLPGQTPVAIIQNAS
                     LPTQRHAVCTLAGLNATLSSKGLASPSVIVVGDVLQGLLALQDKPASHQYSVQHQSAK
                     NLRYLQNSKTEEACICLGA"
     gene            complement(2003..>4494)
                     /locus_tag="PLB25_15570"
     CDS             complement(2003..>4494)
                     /locus_tag="PLB25_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013542431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOE43028.1"
                     /translation="LIGTNNIDSNSRLCMSSAVAGYKQTLGMDSPPACYDDVNHAGTI
                     FIVGANPAYAHPVLFRRIEDARKANPALKIVVADPRRTDSAEIADLYLPILPGTDVML
                     FNGMVHLILWEGWLDTGFIAAHTSGFEALKNTVRDCTPELAAQICGISPEALLQAARL
                     FAGLASSDSARSGSNAMAARSPTLSLYCQGLNQSSSGSAKNAALINLHLATGQIGRPG
                     AGPFSLTGQPNAMGGREVGGMANLLSGHRDLAIPAHRAEVAALWDVPEVPSKPGKTAV
                     EMFQAAADGEIKALWIACTNPAQSMPDQSMVRRALERAEFIVLQEAFATTATCAFADL
                     LLPASTWGEKDGTVTNSERCISRVRAAVPAPAQARHDWQIATDFAQRLQALLPARTPT
                     ARFMHGSLFPYETPESIWNEHRESTRGRDLDITGMSYAMLEAAPQQWPLKAGQTSGQA
                     RLYEDLIFPMPDGRARFVNTRYKPVAEPRQVRYPFSLTTGRLRDQWHGMSRTGTLGHL
                     FGHVSEPGLAMHPRDMARLQLADGDLVQVSSPRAALMVRTQSSPEVAINQAFLAMHWG
                     EEFLGGRSATGERLAGVNALISPVCCPDSRQPEFKHVAVNIRKVDLPWQLLAVAWLPD
                     DGVLAAHAQLQAIMGEFDFASCVPFAEGASTSQRRSGLLFRAAHRQSVEPAVLDKIEA
                     LMQLQGVDILRYADKKRGQHRAIRQSEDETQTLLTGFLLAGDTSAQGWMTTLLKESLP
                     AQTYGRALLLPQARPPLPMASRGQQVCACFDVSDAAINSHLASYTGAAADALASLQQA
                     LKCGTNCGSCMPQVQRLVRAMQTQTAIRLVA"
BASE COUNT          855 a         1375 c         1410 g          854 t
ORIGIN      
        1 cttgggccac atcctgctgc cacagcatag ccccaatgcg atgaacattt ctggctacca
       61 ccgcccatgc cacatagcgc ttgaagccct caatgccgtg atcaaggcaa acatccaggc
      121 catgcactct caatctcctt ggcctgacgg ctacctttgg cgtttatggc ctgctgcgca
      181 aggtggccaa gctaggcgcg ctggagggcc tgacgctgga aaccctgttg ctggccccat
      241 ttgcagtagg cttgctggcg tggtgggcct ggcacggaca gggcgcgctg gtgcacggcg
      301 acgccagcac gctgggctgg ctgctgctgg ccgggctact cacggccatt cccttgctgc
      361 tgtttgccgc tggtgcacgc cgcatcccca tggcgtcccc aaaagggcga agttcgcccc
      421 acgcctgagc caagtccact gcagcgccag cgcgaccaga cgctggagca aatgctcttt
      481 gagcttgaca cagcttgcgc cacgggcaca aagaaaaatg cacaggccta caagatcagc
      541 tggaaaggct acaagctgca cctcgataca gcctgctgtg gcgtgcccat tagcaccgtt
      601 ctgaccggtg cgaacgtggg ttgcatggcc gctcctcacg catcagcgct cagcgggtcg
      661 atacctgcta cgaattgatg gatgcggcct attgcagcac tgtaatccgc gaggacattc
      721 aagctgcggg acgcgtgccc ttgattgacc acaacccgcg caaaggagag aagaaagagt
      781 tcgcgcccga tgaggcagaa cgctacaaga ttcgatcgac ggctgaacgc tgcaatgcgc
      841 gactcaaggc tgaatttgga gcccgtcacg ttcaggtgcg tggccatgcc aaggtgatgt
      901 gccacctgat gctgcgggta gtggcactgt gcgccgatca atttacaagg ttgctgatgt
      961 gacgcaaaaa cacaacaagc tggccaaaat caactctaaa accagttcgc agcgccttgc
     1021 gagggtggcc catgaacaaa aaggataaat tgatgctgta gcgctctgtt ttgtgcccga
     1081 cttctaaaag tgcctaaaaa ttaggcaccg agacagatgc aggcttcttc agtttttgaa
     1141 ttctgcaagt acctcaagtt tttagccgat tgatgttgaa cggagtactg atggctcgca
     1201 ggtttgtctt gcaatgccag caatccttgc aacacatcac cgaccacgat aacgctcgga
     1261 ctggccagtc ccttgctgga caaagtggcg ttgagcccgg ccagcgtgca tacggcgtgg
     1321 cgctgtgtcg gcaggctggc attctgaatg atggccaccg gagtctggcc gggcaggcca
     1381 tgcagcaatt cctgctcaat ctgtgctgca ccgcgcacac ccatgtagat caccagcgtc
     1441 agccgcaggt ggttcgccat gctggccaac ctgggccaat caatgccaag gccacccggc
     1501 ttggtgtggc cagtgacaaa caccaccccc tgtgcctggt cgcggtgcgt cagcggcacc
     1561 cccaaagagc tggctgccgc cagacctgcg gtgatgccgt tgaccacgct gacaaccaca
     1621 ccagccgctt gcaggcgcgc cacctcttca ccaccccgac caaagatgaa ggggtctcca
     1681 cccttcaggc gcaccacgtt ttcaccctcg cgcaccgcca gcaaaatgag cttttcgatg
     1741 aaggcctgcg gggtggattt gcagccgccg cgcttgccga cgcggatgat gcgcgctttc
     1801 gggctggcca gagcgagaat ttcttcgctg atcaaatcgt cagccaacag cacgctggct
     1861 gcctgaatcg ccttgagcgc tttgagcgtc agcagttcgg ggtcgcccgg accggccccc
     1921 accagggtgc atttgccgct cgggccgggg cgcggcacca aggtcaatga agttgcaaag
     1981 cttgtcataa ggtgctgccg attcaagcga ccaaccggat tgctgtctgc gtctgcatgg
     2041 cacgcaccag ccgttgcacc tgtggcatgc aggagccaca gttggtgccg catttcagtg
     2101 cctgttgcaa cgaggccagc gcgtctgcgg cagcccccgt gtaactggcg aggtgcgaat
     2161 tgatcgcggc atcgctcaca tcaaagcagg cgcacacctg ttggccgcgt gatgccattg
     2221 gcagcggtgg cctggcctgt ggcagcagca aggcgcggcc ataggtctga gctggcaggg
     2281 attccttcag caaggtggtc atccagcctt gggcgctggt gtcgccggcc agcaaaaagc
     2341 cggtgagcaa agtttgtgtc tcatcctcgc tttggcgaat ggcccggtgc tggccgcgct
     2401 ttttgtcggc gtagcgcaat atatcgaccc cctgtagttg catcagggct tcgatcttgt
     2461 caagaacggc aggctcgacc gactggcggt gcgcggcgcg aaatagcaaa ccgctgcgcc
     2521 gctggctggt gctggcaccc tcggcaaaag gtacgcaact ggcaaagtca aactctccca
     2581 tgatcgcctg caattgcgca tgggctgcca gcacgccatc gtcaggcagc caggccactg
     2641 ccagcagttg ccagggcaaa tcaaccttgc gaatgttgac ggcaacatgc ttgaactcgg
     2701 gttgccggga gtccgggcag cagactggcg agatcagcgc attgaccccc gccaggcgtt
     2761 cgccggtggc actgcgcccg cctaaaaact cttcgcccca gtgcatcgcc agaaaagcct
     2821 ggttgatggc gacctctgga ctgctttggg tgcgcaccat gagggccgcg cgtgggctgc
     2881 tgacctgcac caggtcgccg tctgccaact gcagccgggc catgtcgcgc ggatgcatgg
     2941 ccaggccggg ttcgctgaca tggccaaaca aatggcccaa ggtgccggtg cggctcatgc
     3001 cgtgccattg gtcgcgcagc cggccggtgg tcagcgaaaa cgggtaacgc acctggcgcg
     3061 gttcagccac cggcttgtag cgggtgttga caaagcgggc tctgccgtcg ggcatgggaa
     3121 aaatcaagtc ttcgtacaac cgcgcctggc cgctggtctg gccggcttta agcggccatt
     3181 gttgcggcgc ggcttccagc atggcgtagc tcatgccggt gatgtcgagg tcacgaccac
     3241 gggtcgattc gcggtgctca ttccagatgg attctggggt ttcgtagggg aataagctgc
     3301 catgcataaa acgcgctgtc ggcgtgcgag caggcaacaa ggcttgcagc cgctgcgcaa
     3361 agtcggtggc aatctgccaa tcgtgacgcg cctgcgcagg cgcgggcacg gccgcgcgaa
     3421 cccggctgat gcagcgctct gaattggtca ccgtgccgtc cttctcgccc caggtcgagg
     3481 ctggcagcag cagatcggca aaggcgcagg tggcggtggt ggcaaaggcc tcttgcagca
     3541 cgatgaactc ggcccgctcc aaagcacgcc gcaccatgct ctggtcgggc atgctttgcg
     3601 ccgggttggt gcaggcaatc cacagcgcct tgatctcgcc atcggctgcc gcctgaaaca
     3661 tttcaaccgc tgttttgccc ggtttggacg gcacctctgg cacgtcccac agcgcagcca
     3721 cctcggcgcg gtgggccggg atggccaggt cgcggtggcc actgagcagg ttggccatgc
     3781 cgccaacttc gcgcccgccc atggcgttgg gctggccggt cagcgaaaac gggccggccc
     3841 caggccgacc aatttggccg gtagccaggt gcaggttgat cagcgcggca tttttggcgc
     3901 tgccgctgct ggactggttc agcccctgac agtacaggct aagggtgggg ctgcgggcag
     3961 ccattgcgtt tgacccagac cgggcgctgt cgctgcttgc caggccggca aacagccggg
     4021 cggcttgcag cagcgcctct gggctgatgc cgcagatttg cgctgccagc tcgggggtgc
     4081 agtcgcgcac ggtgttcttg agggcctcaa agccgctggt gtgggcggcg ataaagccgg
     4141 tatcaagcca gccctcccac aggatgaggt gcaccatgcc gttgaacagc atcacgtcgg
     4201 tgccgggcaa gattggcagg tacaggtcgg caatttcggc gctatcggtg cggcgcgggt
     4261 cagcaacgac gattttcagc gccgggttgg ccttgcgggc atcttcaatg cgccggaaca
     4321 ataccggatg ggcataggcc gggttggcgc cgacgataaa aatcgtgccg gcatggttga
     4381 catcgtcata gcaggcaggc ggcgagtcca tgcccagcgt ctgcttgtag ccggccaccg
     4441 cgctgctcat gcacaggcgc gagttgctgt cgatgttgtt ggtgccgatc aggc
//
LOCUS       DAOSLL010000399         4493 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17098_length_4493_cov_4.756422, whole genome shotgun sequence.
ACCESSION   DAOSLL010000399 DAOSLL010000000
VERSION     DAOSLL010000399.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17098_length_4493_cov_4.756422"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..1189)
                     /locus_tag="PLB25_15575"
     CDS             complement(257..1189)
                     /locus_tag="PLB25_15575"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOE43029.1"
                     /translation="MATQSDPNHPTPDFSASLAPQMAVVFADVAGSTRMYDTLGDTRA
                     KELVDECLALMTEVVKQHNGRVIKTIGDEVMCVLPSADSGCLAAMKMQLGIDELLAYG
                     SIKRAIRVGFHFGPVIEEKNDVFGDTVNLAARMAGLAKAQQIITTASTVQQLSPAMRF
                     ITRRIAALSVKGKGDDIEVYDVIWQPNDSVTIMAPAFNSIARVAELHLQHGNTPLVLT
                     QTDSAIVLGRDAGCDLVISGHTVSRQHARIERRRDKFFLIDQSTNGTFLSIVGEPEIT
                     LRREEVVLRTQGRIALGHSVDLMTDEVITFAVRG"
     gene            complement(1703..3322)
                     /locus_tag="PLB25_15580"
     CDS             complement(1703..3322)
                     /locus_tag="PLB25_15580"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HOE43030.1"
                     /translation="MLDYLIIGGGSAGCVLASRLTENPDICVALLEAGPVDSSVLIHC
                     PAGLAGLAKFSLLGWNLNTVPQPGLNGRSGYQPRGKVLGGSSSLNAMVYVRGQRADYD
                     HWSALGNPGWSFDEVLPYFKRAEHNERGADAFHGSGGPLNVMDLRSPNRFGNLFVQAG
                     AQAGFKVNADFNGADQEGVGTYQVTHKNGERCSAAKAYLTPNLARPNLHVLTGAHTTR
                     ILLERKRAVGVEFVHEGQLKQLKCQREVLLCAGAIQSPQILMLSGIGPHQHLVDRGIA
                     TQHNLPGVGQHLHDHPDIVQVVNVPQAKDLFGLSLTGAVKTLKGIFEWRKLRSGMLTT
                     NFAEAGGFIKSQPGEATPDLQLHFVIGKLVNHGRTLTMGHGFSCHVCILRPLSRGSVT
                     LASKDPMSLPLVDPDFLGHRDDMDRLIRGFKLMRNILAQPALAQFGGRELDSTAGAQT
                     DAQIEQVIRDHADTIYHPVGSCRMGNTDMDVVDAQLRVHGMQGLRVVDASIMPRVVSG
                     NTNAPVMMIAEKAADMIRADQAAATAAAVRC"
     gene            3507..>4493
                     /locus_tag="PLB25_15585"
     CDS             3507..>4493
                     /locus_tag="PLB25_15585"
                     /inference="COORDINATES: protein motif:HMM:NF017009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43031.1"
                     /translation="MSESLSSETAAPVAPPASIFGWPPPPFADYSAAAILLEPQECDI
                     EGSIGGSRHCTLFAVVPTEKKILIQIERARSMVSLAFSNFHRLILNTALEPVKQTGNK
                     SPADKSIDVQASRYLLYLVDGSILSGLTLGHVQMPFGLFLYTPVNDLGHLQLEFIPSE
                     AYLRLEFVGNDRVIAAPALELPKPTVSPLMPAEATASPESEPKQEEALAEALVTSPSQ
                     LLQALDSQARMPLLRLGEALRHIAYVTENQLQQALDLQKQNPVEPLGELLIRIGFLTR
                     KKLQAAMVRKMGYPVVDVTHFPIDPKALEKVNMATALRLRIVPLLARPNLLVV"
BASE COUNT          884 a         1356 c         1293 g          960 t
ORIGIN      
        1 ctgtttgtgt aggagtcgcg ccctcgcgac gaaggccgtc caattccggc tcgtctggct
       61 taggcaatca cgtcaactgc cctggaccag gatggcgcaa ccgcaatggg caggtttgac
      121 ggcacatgtc gccgaaggcc tttcgggcac cctcgcatgc gttaccaacc atttgaccag
      181 gcaccccggc ttcggcgtga ccaggctcga agggcttgtt acccccggcg aaccctgcac
      241 cggcgctgca ttgcccctat ccgcgcactg cgaacgtgat gacttcgtcg gtcatcaggt
      301 ccacgctatg ccccagagca atgcgaccct gcgtgcgcag cacgacctcc tcgcgccgca
      361 atgtgatctc tggttctcca acaattgaca aaaaggtgcc atttgtgctt tggtcgatca
      421 gaaaaaactt gtcgcgccgt cgctctatgc gcgcgtgctg tcgcgaaacg gtatggccac
      481 tgatcaccag gtcgcatccc gcatcccgcc ccagcacaat ggcactgtcg gtttgtgtca
      541 gcaccagcgg ggtgttgccg tgctgcagat gaagttctgc gaccctggcg atgctgttaa
      601 acgctggtgc catgatcgtc actgaatcat tgggttgcca gatcacatca tagacttcga
      661 tatcgtcgcc cttgcccttg accgacagcg cagcgatgcg ccgggtaatg aagcgcatgg
      721 caggtgacaa ttgctgtacc gtcgaggccg tcgtgatgat ctgttgggcc ttggccaagc
      781 ccgccatgcg cgctgccaga ttgacggtgt cgccaaaaac gtcgtttttc tcttcgatca
      841 ccgggccgaa gtgaaacccc accctgatgg cgcgcttgat acttccgtaa gccagcagct
      901 catcgatgcc aagttgcatt ttcattgccg ccaggcagcc gctgtcagcg ctgggcaaca
      961 cgcacatcac ttcgtcgccg atggtcttga tgacgcgtcc gttgtgctgc tttaccacct
     1021 cggtcatcag tgcaaggcac tcgtcaacca gttctttggc acgggtatct cccagcgtat
     1081 cgtacatccg ggtgctaccg gcaacgtcgg caaacacgac cgccatctgc ggtgccagcg
     1141 acgcggaaaa gtcaggagtg ggatggttcg ggtcagactg ggttgccata ttgcgtactt
     1201 gatcaagagc agggttggac agagccgatt atggggttgc gcatcgcgtc cgggcaagca
     1261 aacaccagca tatcgcttga tttgtggccc ggacaccggg gcctgggagc ctgcttggct
     1321 tatggtgcat cggctgcgca ccgaccgcag cagccctttt tgcgtcggct ttttgaccca
     1381 caactgggct gtgggtcggc aattccggca caaactggca tcgctgggcc tgattctcgg
     1441 tccccgattc atcagttcga cagactcctg gaaattctag taccgacaag cccggcgctt
     1501 ttgcaaattg tgatgctgat cttccctttt acctgtctgg cctgcagagc gaggcgacgc
     1561 acctgccggg gtgatatgcc ggattgcaaa tgccacgccg acccaatcag tcagtttgtt
     1621 gctttgcgtg acctcggccg cgagcgagcg catcggcgca acgccaatcg ggcacatcaa
     1681 aaaaactcgg tggcgcgcgt gatcaacacc gcactgcggc agcggtcgcg gctgcctggt
     1741 cagcccggat catgtcggct gctttttccg caatcatcat caccggtgca ttggtgttgc
     1801 cgctgaccac gcgaggcatg atggatgcat cgaccacccg caagccctgc atgccgtgca
     1861 cgcgcaactg cgcatcaacc acgtccatgt cggtattgcc catgcggcaa ctgccgacgg
     1921 ggtggtagat ggtgtcagca tggtcgcgga tcacctgctc tatctgtgca tccgtttgcg
     1981 cgcctgccgt ggagtcaagc tcgcgcccgc caaactgcgc caacgcgggt tgcgccagaa
     2041 tgttgcgcat cagtttgaag ccgcgaatca ggcggtccat gtcgtcgcgg tggcccaaaa
     2101 agtcggggtc taccaagggt agcgacatcg gatctttgct ggccagcgtg acactgcccc
     2161 ggcttagcgg tcgcaggatg cagacatggc aggaaaaacc gtggcccatc gtcagggtgc
     2221 ggccatggtt gacaagcttg ccgatgacaa agtgcagttg cagatcgggc gtggcttctc
     2281 ccggctggct tttgataaaa ccaccagcct cggcaaagtt ggtggtcaac atgccgctgc
     2341 gcagcttgcg ccactcgaaa atgcccttca gggtcttgac agcgccggtc agcgacagtc
     2401 caaacaggtc tttcgcctga ggcacgttca ccacctgcac gatgtccggg tggtcatgca
     2461 agtgctgccc gacgcctggc aggttgtgct gggttgcaat gccgcgatcc accaggtgct
     2521 ggtgtgggcc gatacccgaa agcatcaaga tttgcggtga ctggatggcc ccggcgcaca
     2581 gcagcacctc gcgctggcac ttgagttgct tgagttggcc ctcatgcacg aactccacgc
     2641 cgactgcacg ctttctctcc agcagtattc gggtggtgtg ggcaccagtc aacacatgca
     2701 ggttcgggcg ggccagattt ggtgtcaggt atgccttggc cgcgctgcag cgctcgccat
     2761 tcttgtgcgt cacctggtag gttccgacac cttcctgatc cgccccattg aagtcggcat
     2821 tgaccttgaa gccagcctgc gcgccagctt gcacaaacaa gttgccaaat ctgtttgggc
     2881 tgcgcaaatc cataacattg agcggtccac cgctgccgtg gaaggcatct gctccacgtt
     2941 cgttgtgttc ggcgcgtttg aaatagggca acacctcgtc aaacgaccag cctgggttac
     3001 ccagcgccga ccaatggtca tagtcagccc gctggccgcg gacataaacc atggcattga
     3061 gtgaactcga cccgcccagc accttgccgc gtggctggta gcctgacctg ccgttcaggc
     3121 ccggttgcgg caccgtgttc aggttccagc ccagcaggct gaacttggcg agcccggcca
     3181 gaccggccgg acaatgaatc agcacgctgc tgtccaccgg tccggcttcg agcagggcta
     3241 cgcagatgtc agggttttca gtcaggcgtg acgccaatac gcagccagca gagccgccgc
     3301 cgatgatgag gtagtcaagc ataaaaaatc tccgggcgcg gccagatagc tgggtggcaa
     3361 gcctgcgctg tacgttaccg taaaacgaca tggtgaatta gagacttacc tcaatcctgg
     3421 ccttcctcaa aaggcaaaaa atcgggcatg atgcgtctat gttgtcaacg ccaatcaatc
     3481 cctcgcgtga tccgacgaaa ccgctggtgt ctgaatcgtt gtccagcgaa actgctgcgc
     3541 cggtcgctcc gcctgcgtcg atttttggat ggccaccacc gccatttgcc gactacagcg
     3601 cggcggcaat tttgttggag ccgcaagaat gtgacattga gggctccatt ggtggcagtc
     3661 ggcactgcac cctgtttgct gtcgtcccga ctgaaaagaa aatcctgatt cagattgagc
     3721 gcgccaggtc gatggtgtcg ctggcttttt caaattttca tcgtctgatt ctcaatacgg
     3781 cccttgaacc agtcaagcaa acaggcaata agtcccctgc tgacaaatcg attgacgttc
     3841 aagcatcccg ttaccttctg tacctggttg atggcagcat cctgtcaggc ctgacgctgg
     3901 ggcatgtgca gatgcctttt ggcctgtttt tgtacacgcc ggtgaacgac cttggccact
     3961 tgcagcttga gttcatcccg agcgaggctt atcttcgcct ggagtttgta ggtaacgacc
     4021 gcgtgattgc cgcgccagcc ttggaactgc ccaagccaac agtgagcccg ctcatgcctg
     4081 ccgaggcaac tgccagtccc gagtcagagc cgaaacaaga ggaggctctg gccgaggcgt
     4141 tggtgacttc gccgagccag cttctgcagg cgctagacag tcaggccaga atgccattgc
     4201 tgcggttggg tgaagccctc aggcacatcg cctacgtgac cgagaatcag cttcagcaag
     4261 ccctcgatct gcagaaacag aaccccgtcg agccacttgg ggaactgctg atccgaatcg
     4321 ggttcttaac ccgcaagaag ctccaagccg ccatggttcg caagatgggt tacccggtgg
     4381 ttgatgtcac ccacttcccg attgatccaa aggcgttgga aaaagtcaac atggcgacgg
     4441 ctttgcgcct gagaattgtg ccgctgctgg cgcgccccaa cctgctggtg gtg
//
LOCUS       DAOSLL010000400         4490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17107_length_4490_cov_3.783766, whole genome shotgun sequence.
ACCESSION   DAOSLL010000400 DAOSLL010000000
VERSION     DAOSLL010000400.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4490
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17107_length_4490_cov_3.783766"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..2208)
                     /locus_tag="PLB25_15590"
     CDS             complement(43..2208)
                     /locus_tag="PLB25_15590"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOE43032.1"
                     /translation="MLNDALILLRRGVASSVSMLAPLLAVFISAGLLLIDPPLMQTLR
                     HAVFDQYQRAYPRTYQPVAVRIIDIDEESLSKIGQWPWPRTRIAELVHLLQQAEVAAV
                     GFDVLFAEPDRTSPKAMLDIWTLPAATRRDVARLPDHDEWLAKTIKPGGVVLGFAVER
                     SGQGAELPAQKYRFVSVGEPALPFVHPFSRAVPSLPLLSAAAAGNGALTFVPDNDGVV
                     RRVPLLLRLDDALVPSLVAEVLRVGQGASNYMVRTSPTQGVGLTEVRVGQLTVPTTPA
                     GEVWVHYTGAVKERTIPAWKVLTGTVSAADLKGTIVLVGTSAQGLMDLRFSPLGGVMP
                     GVEAHAQMLEQVLSDSYLNRPAWALSIEALTIVIGGLALGTFALVTGAVLSASVTLLT
                     IGLIIGGGWLAFIQYQLLLDPVTPALGIFLAFLLSSVLHHLSSERRQRFVRAAFSRYV
                     SPNLVTHIVEHPAELELGGRRQDCSFVFTDLAGFTSLMEKIDPGEAVALLNVYLDEMI
                     GIAFAHNGTLDRIVGDAVAIMFSAPVQQFDHRQRALNCAIAMQRFATRYADDLKAKGI
                     AFGHTRMGVHSGEVIVGNFGGSTIFDYRALGDPVNTASRLESVNKHLGTRICVSEATL
                     AGCTGVVTRPVGRLVLKGKSQPLMVFQPIHDGLELAGASTEDPDYAAAYALLTQDTQA
                     AIRAFEQLAKARPNDPLVILHLHRLQEKHDGDLIVMTEK"
     gene            2738..2956
                     /locus_tag="PLB25_15595"
     CDS             2738..2956
                     /locus_tag="PLB25_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43033.1"
                     /translation="MKDLEVELLATFVEFDRHKGICDKSAKWINLDCTAWCGKRSINA
                     KPLIALRHLAYSATQELRRPSDSVKGMQ"
     gene            complement(3033..3119)
                     /locus_tag="PLB25_15600"
     tRNA            complement(3033..3119)
                     /locus_tag="PLB25_15600"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3083..3085),aa:Leu,seq:tag)
     gene            complement(3244..>4490)
                     /locus_tag="PLB25_15605"
     CDS             complement(3244..>4490)
                     /locus_tag="PLB25_15605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOE43034.1"
                     /translation="PLDQIFPQSNVSQLVLLPKDLATRELLRVKLPTILAQEFPEVRG
                     RVKLLANGPPVPYPVQFRVIGTDPQVLRVLADEVNAALRQNTNTRGVNDNWNESIKVM
                     RLEVDQTKARALGVSSQAIAQAAKTMLAGATVGQFREGDKLIDIVLRQPLEERRAITD
                     LANAYLPTASGRSVPLTQIAKPVFTWEPGVMWREGRDYAITVQADIAEGLQGATVTAQ
                     LMPELKLLEDRWVQAGHGAYRIEVAGAVAESSKGSSSIVVGIPVMLFITFTLLMLQLH
                     SFSRAMLVFLTGPLGIAGVAASLLLLNRPFGFVALLGVIALMGMIQRNSVILIDQIEQ
                     DRARGVPAWDAIVESAVRRLRPIVLTAAAAVLAMIPLSRSVFWGPMAVAIMGGLIVAT
                     ALTLLALPATYAAWFKVTPQSK"
BASE COUNT         1045 a         1372 c         1203 g          870 t
ORIGIN      
        1 ggcgatgcgg tgccaggcta cccgcgcagc ccgaatagtt gcttattttt cagtcatcac
       61 aatcaggtca ccatcgtgct tctcttgcag ccggtggagg tgcaatatga ccaaggggtc
      121 attgggcctg gctttggcca gttgctcaaa agcgcgtatt gcggcctgtg tatcctgggt
      181 taacaaggcg taggcggcag cgtaatctgg gtcttctgtt gaagccccgg ccaactccag
      241 cccatcatgg attggctgaa ataccatcag aggctgcgac ttgcccttga gtaccaggcg
      301 ccccaccggt cgcgtcacga caccagtgca acccgccagg gtggcctctg acacgcagat
      361 tcgggtgccc aggtgtttgt tgacactctc cagccgagaa gctgtattga ccggatcgcc
      421 cagagcacgg taatcaaaaa tggttgatcc accaaaattc ccgacaatca cttcgccgct
      481 gtgaacaccc atgcgggtgt gaccaaaggc aatgcctttg gccttcaaat cgtccgcgta
      541 acgcgtggca aatcgttgca tggcaatggc acagttcagc gcacgttggc ggtggtcaaa
      601 ttgctgcacc ggtgccgaaa acatgatcgc aacggcatcg ccaacgatac gatcaagcgt
      661 gccattgtgg gcgaacgcga tgccaatcat ctcgtccaga tagacgttaa gcagggcaac
      721 cgcctcgccc gggtcaattt tctccatcag gctggtgaag cccgcaaggt cggtgaatac
      781 aaaactgcaa tcctgccgcc gtccacccaa ctcaagttct gctggatgtt caacaatgtg
      841 agtcaccaga ttgggcgaca catagcgcga aaacgcggcc cgaacaaagc gctggcgacg
      901 ctcgcttgag agatggtgga ggacactgga cagcaggaag gcgaggaaga tgcccagcgc
      961 tggcgtaacc gggtccagca gtagctgata ttgaatgaac gccagccagc caccgccaat
     1021 aatcaggcca atggtcagca aggtaacact ggcagacaaa accgcaccgg tcaccagcgc
     1081 aaacgtgccc agcgccaagc caccaatgac aatggtcagg gcttcgatcg aaagagccca
     1141 ggccggacgg ttcagataac tgtcggacag cacttgttcg agcatctgcg catgtgcctc
     1201 aacccctggc ataacacctc ccagcgggct gaaccgcagg tccatcaagc cctgcgcaga
     1261 ggtacctacc aacacaatcg ttcccttcag atctgccgct gaaacggtcc cggttaaaac
     1321 cttccaggcg ggtatggtgc gttcttttac agcaccggtg taatgcaccc atacctcccc
     1381 ggccggcgtg gttggtaccg tcagttgccc caccctgact tcggtcagcc caaccccttg
     1441 cgtcggcgag gtacgcacca tgtagttgct tgcgccttgc ccaacccgta atacttcagc
     1501 caccagcgac ggcaccaagg catcatccag gcgcaacagc agcggtaccc ggcgcaccac
     1561 accgtcgttg tcgggcacga aagtcaaggc accattgcca gcagcagcgg cactcaacaa
     1621 gggcagcgac ggcacggccc tggaaaaagg atggacaaag ggcagcgcgg gttcgcccac
     1681 gcttacaaag cggtatttct gcgcaggcaa ctcggcaccc tggccactgc gctcaacggc
     1741 gaaccccagc acaacaccgc caggcttgat ggttttggcc aaccattcat catgatctgg
     1801 caagcgggcc acatcgcgac gcgttgctgc tggcaatgtc cagatatcga gcatcgcctt
     1861 gggagaagtg cgatccggtt cggcaaacag cacgtcgaac ccgactgcgg caacctccgc
     1921 ctgctgcagg agatggacca attcggcgat tcgggttcgc ggccatggcc attggccaat
     1981 cttgctcagg ctctcctcat caatgtcaat gatgcgcacg gcaaccggct ggtaggtgcg
     2041 tggataggct cgctggtact ggtcaaacac cgcatggcgc agcgtttgca tcaagggcgg
     2101 atcaatcaac agcaaaccgg cactgatgaa aaccgccaac agcggagcga gcatgctcac
     2161 gctgctggca acaccgcggc gcagaagaat cagtgcatcg ttcaacatcg gcttgttttc
     2221 agcttgtcaa atggctcggt tttcaaaagt ggtccgtttc taatgtacca atgaaatcaa
     2281 aacacactgg actacaagca ggagtcagca tgatttgttc ggctcgactt ctggcccaac
     2341 attcacgctt tcgagaccaa tttccggcag cctccaggct gcagtaaggg tttgcgggcg
     2401 cctatttgca gttcgtagcg tcgaagcctg gcgcgagagg tctttttgtc ttgccatcag
     2461 ccagacctcg cttccacacc cactgatcga gccaaccatg cagaatttcc acgctcggtt
     2521 ctccgatttg attcagtaca aaagtctcgg catcggctgc tatcgggcac ctctgaattc
     2581 atgcacgctc ctctgcgatg cggcccttcc attgccacca gattgactaa cgcatgaaag
     2641 ctacctgcca gtgttgttcc acttctaaat catccgtgtc taggcaccct gccgcagaca
     2701 ttgcaaaacg caaggcatat ggcgaaacaa caacgacatg aaagatctag aagtggaatt
     2761 actggcgacc tttgttgaat ttgatcgcca caaaggaatc tgcgacaagt cggccaaatg
     2821 gatcaatctc gattgcactg cttggtgcgg caagcggtcc atcaacgcaa agccactcat
     2881 cgcgctaaga catttggctt actccgcaac gcaagaactt cgccgacctt cggattcagt
     2941 gaaaggaatg caatgaaacc ggatgggaag gtcattcatt tcactcaaca accaaaactt
     3001 cagctaagtt gttgattttc gtacgttttc agtggtgcgc ggggcgggac tcgaacccgc
     3061 acagtattgc taccgtcagg acctaaacct ggtgcgtcta ccaatttcgc cacccgcgca
     3121 aatcaaaagg ggccggtaaa acactacccc agaaaattgg ttagccgacc attttacccc
     3181 gctggagagg ctcaatgcag ggtcaattca gccaaatatg ccaggtcgca tctgcgcaag
     3241 ccgtcatttg ctctggggtg tcaccttgaa ccaggccgca tatgtggccg gcaaagccag
     3301 cagagtcagt gccgtagcca caatcaaccc gcccatgatg gccaccgcca tcggccccca
     3361 aaataccgag cgcgacagcg gaatcatggc cagcacggcg gccgcagcag tcaacacaat
     3421 cgggcgcagg cggcgcacgg cagattcaac aatcgcatcc caggctggca cacctcgggc
     3481 ccggtcttgc tcgatttggt caatcagaat caccgaattg cgctgaatca tgcccatcag
     3541 ggcaatcacc cctaacagcg caacaaagcc aaacggccgg ttcaacagca acagggatgc
     3601 tgccaccccg gcaatgccca atggtccggt caaaaacacc agcatggcgc ggctgaaact
     3661 gtgcaactgc agcatcagca gcgtgaacgt gatgaacaac atcaccggga tacctaccac
     3721 aatcgacgac gagcccttgg agctctcggc cacggccccg gcaacttcaa tgcggtaagc
     3781 gccatgcccg gcttgcaccc aacggtcctc cagcagcttg agctctggca tcagttgtgc
     3841 cgtcacggtg gcaccttgca gcccctcggc aatgtcagct tgcactgtga tggcgtagtc
     3901 gcgcccctct cgccacatca cgcccggctc ccaggtgaag actggtttgg cgatttgcgt
     3961 cagaggcacc gacctgccgg atgccgtcgg caaatacgca ttggcaaggt cagtaatcgc
     4021 tcggcgttcc tctagcggct ggcgcaacac gatgtcgatg agcttgtcgc cttcgcggaa
     4081 ctgcccgacc gtggcacccg cgagcatggt cttggcggcc tgcgcgatgg cctgactgct
     4141 tacccccagc gcacgcgcct tggtctgatc cacttccagc cgcatcacct tgatggattc
     4201 gttccagttg tcattcacac cacgggtgtt ggtgttttgg cgtagcgctg cgttgacctc
     4261 atcggccagc acccgcagca cctgcgggtc agtgccaatc acacgaaatt gcactgggta
     4321 aggcaccggc ggaccattgg ccagcagctt gacgcggcca cgcacctcag gaaattcctg
     4381 cgcaaggatg gttggcagtt taacccgcaa cagttcgcgg gtggccaggt ctttaggcag
     4441 cagcaccagt tgcgagacgt tggactgcgg aaagatctgg tccagcggca
//
LOCUS       DAOSLL010000401         4474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17189_length_4474_cov_4.740892, whole genome shotgun sequence.
ACCESSION   DAOSLL010000401 DAOSLL010000000
VERSION     DAOSLL010000401.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17189_length_4474_cov_4.740892"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="PLB25_15610"
     CDS             complement(<1..687)
                     /locus_tag="PLB25_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOE43035.1"
                     /translation="MIPVKTESAWRGTSDCRKCGIRDMVLFANLNEHDFAQMHVPIDD
                     LELSQGSFLYTEGSPAQSVFTVRAGMLKLVRSTLDGRQRIVRVLRCGDVIGLEALATT
                     HYDCDAVALTQVAVCRIPLGVIQKLSSGSGRLLSQLMLKWQQTLKDADDWLADLNFGT
                     ARQRVGNLVLKMRNPDNGEIAMLFSREDMGAMLDLKLETVSREISRLVREGSIEPLDK
                     QGRIYRVCHPD"
     gene            complement(790..1128)
                     /locus_tag="PLB25_15615"
     CDS             complement(790..1128)
                     /locus_tag="PLB25_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HOE43036.1"
                     /translation="MAIQLQEDGTSGMLQSDATFESAAELFRVMSAPMRLKIINCLCD
                     GEKNVSYLLTKVNTTQPNMSQHLNTLYRAGVLGKRRDGVQIFYRIADQRIVSICQAVC
                     HDIASKPEPT"
     gene            complement(1302..2609)
                     /locus_tag="PLB25_15620"
     CDS             complement(1302..2609)
                     /locus_tag="PLB25_15620"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HOE43037.1"
                     /translation="MNKQTNLLIRGGRVIDPAREFDENADVALAGNALVAIGNIAPEF
                     QPDIVLDATGCLVLPGLVDLAVHLREPGYEHARMLESEMAAAMAGGVTSLVCPPDTDP
                     VLDESGLVEMLRFRAEKLQQARVYPLGALTRNLQGATLTEMLELTEAGCVAFSQAELG
                     LSNNLVLQRAFQYASTYGYAVWLRAQDSFLGQGVAASGPLATRMGLSGVPVIAETIAM
                     HTIFELMRGTGARVHLCRISSADGVALVRQAKAQGLALSCDVSINSLHLTDNDIGYFD
                     SRARLNPPLRQQRDRDALRAGLADGTIDALVSDHTPVDDDAKALPFAQSEPGATGVEL
                     LLSLAYKWHLDTGVKLSRALAVVTAGPAAVLGNTLGTRQGRTGHLMAGGLADMCVFDP
                     RADWVVCAAALVSQGKHTPFSGYELPGRVRYTLVGGRVAFQAK"
     gene            complement(2640..3614)
                     /locus_tag="PLB25_15625"
     CDS             complement(2640..3614)
                     /locus_tag="PLB25_15625"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HOE43038.1"
                     /translation="MLYKRNPQLNKNGELIHLLSTEGLPKSILTQVLDTATNFVSVSD
                     REVKKVPLLRGKSVFNLFFENSTRTRTTFDIAAKRLSADVINLDIAKSSASKGETLLD
                     TIANLSAMAADIFVVRHSESGAPYLIAQHVAPHVHVINAGDGRHAHPTQGLLDAYTIR
                     HYKQDFSNLTVAIVGDVLHSRVARSDIHALTTLGCAEVRVVGPKTLVPSDMAQMGVRV
                     CHTLEEGIRGCDVVIMLRLQNERMSGALLPSSQEFFKSFGLTQEKLRLARPDAIVMHP
                     GPINRGVEIDSAVVDGRQSVILPQVTFGIAVRMAVMSIVAGNDATHTA"
     gene            complement(3617..4099)
                     /gene="pyrR"
                     /locus_tag="PLB25_15630"
     CDS             complement(3617..4099)
                     /gene="pyrR"
                     /locus_tag="PLB25_15630"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HOE43039.1"
                     /translation="MDAESLYSELVHSVRAIFRPGMRLLGVTSGGAWLAQRLQQDLGL
                     TEPVGVISSAMHRDDFARRGMTAAAQTKIPFDVNNAQILLLDDVLFTGRTIRAVINEL
                     YDFGRPAKVILAVLVDRGGRELPIQPDICAARIHLPASQSLSLARDASDKFSFNLKAH
                     "
     gene            complement(4189..>4474)
                     /gene="ruvX"
                     /locus_tag="PLB25_15635"
     CDS             complement(4189..>4474)
                     /gene="ruvX"
                     /locus_tag="PLB25_15635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HOE43040.1"
                     /translation="TAEGDARFVKIARHLKEWQPDALVVGVPFHPDGAAHDNTARARR
                     FARQLHGRFKLPVFEVDERYTTTEAIGNGARDADAEAACIILEQFLRNLP"
BASE COUNT          904 a         1396 c         1309 g          865 t
ORIGIN      
        1 atccggatgg catacgcggt agatgcgccc ctgcttgtcg agcggttcta tgctgccctc
       61 gcgcaccaga cggctgattt cgcggctgac ggtttcaagc ttcaggtcca gcatcgcgcc
      121 catgtcctcg cgtgaaaaca acatggcgat ttcaccgttg tccgggttgc gcatcttcag
      181 caccagattg ccgacccgct ggcgcgctgt gccaaagttc agatcggcca gccagtcatc
      241 agcgtctttg agcgtctgtt gccatttgag catcagttgg gacagcaggc ggccactgcc
      301 gctggagagc ttctgaatca ccccgagcgg gattcggcaa accgccactt gggtcaaggc
      361 gacggcatcg cagtcatagt gtgtggtggc cagcgcctcc aggccaatca catccccgca
      421 gcgcagcacc cgaacaatac gttgcctgcc atcgagcgtg ctgcgcacca gtttcaacat
      481 gccggcgcgc acggtaaaga cactctgcgc cgggctgccc tcggtataca gaaaagaccc
      541 ctgagaaagt tccagatcat caatcggaac atgcatttgg gcaaaatcat gctcgttcaa
      601 attggcgaac aagaccatgt cacgaatgcc gcacttgcgg caatctgaag tgccacgcca
      661 ggcggattcg gttttgacag ggatcatgaa tcagctttcc aaatgttatc catgaccctt
      721 gccgcgctga tgcgtgcggg ccaggcccgg ggccgagtca ctgagcaggc cacctgtcac
      781 cggtttgcgt caggtgggtt cgggcttgct ggcgatgtca tgacagaccg cctggcagat
      841 tgacacgatg cgctgatcgg cgatgcggta gaaaatctgc acaccgtcgc ggcgtttgcc
      901 caacacgcca gcccgataca aggtgttgag gtgctgcgac atgttgggct gggtggtatt
      961 gaccttggtg agcaggtaac tgacgttttt ttcaccatcg cacaggcaat tgatgatttt
     1021 cagccgcatc ggtgccgaca tcacacgaaa cagctcggcg gccgactcaa acgtcgcatc
     1081 agactgaagc attcctgatg tgccatcttc ctgcaattga atcgccatgc caatatgtcc
     1141 ttgtgaaacc ccagtgtgga tcatccgccc gacatcatgt ctgcgcatgc ttcaaagaag
     1201 agcttgcgcc agacaggaat tgtagtctgg cggtcgccgt ttcacgcctg gcagcaacag
     1261 cgtccggtca acgccagcgc cgcaccgcac accaaactga atcactttgc ctggaatgcc
     1321 acgcgtccgc cgaccagcgt gtagcgcacc cgaccaggca gctcatagcc tgaaaacggc
     1381 gtgtgcttgc cctggctgac cagcgccgca gcgcacacca cccaatcggc acgcggatca
     1441 aacacacaca tatcggccaa accaccagcc atcagatgcc cggtgcggcc ctggcgcgtg
     1501 cccagtgtgt tgccgagcac cgcagccggc ccggcggtca ccacggccaa ggcccggctg
     1561 agcttgaccc cggtgtcaag atgccacttg taggccaggc tgagcagcag ttccaccccg
     1621 gtggcaccgg gctcgctctg ggcaaaaggc aatgccttgg catcgtcatc gaccggtgtg
     1681 tggtccgaca ccagcgcatc gatggtgccg tcagccagac cggcgcgtag cgcatcgcgg
     1741 tcgcgttgct ggcgcaacgg cgggttcaga cgggcccggc tgtcaaagta gccaatgtcg
     1801 ttgtcggtca ggtgcagtga attgatgctg acatcgcaac tcagtgccag cccctgcgcc
     1861 ttggcctggc gcaccagcgc aaccccgtcc gcactgctga tgcggcacag gtgcacccgt
     1921 gcgccggtgc cgcgcatcag ctcgaaaatg gtgtgcatgg caatggtctc ggcgatcacc
     1981 ggtacgccac ttaaacccat gcgggtcgcc agcggcccac tggccgcgac gccttgcccc
     2041 aaaaaggaat cctgggcgcg tagccagacc gcatagccat aggtgctggc atattgaaat
     2101 gcgcgctgca acaccaggtt gttgctcaag ccgagctcgg cctgactgaa cgcgacacaa
     2161 cccgcctcgg tcagttcaag catttcagtc agcgtggcac cttgcaggtt gcgcgttagc
     2221 gcgcctagcg gataaacccg cgcctgctgc aatttttcgg cgcgaaagcg cagcatctcg
     2281 accaggccgg actcgtcaag caccgggtcg gtgtcaggcg ggcacaccag gctggtcaca
     2341 ccgccagcca tggcggctgc catttcggat tcgagcatgc gcgcatgctc gtaaccaggc
     2401 tcgcgcaggt gcactgccag gtccaccaga cccggcaaca ccagacagcc agtggcatcc
     2461 agaacgatgt ccggctgaaa ctccggcgct atatttccaa tagctaccag cgcattgcca
     2521 gcaagggcta catcggcatt ttcatcaaat tcacgggccg ggtcgatgac cctgccaccc
     2581 cggatcaaca gattggtttg cttgttcatg gtgcgtctat tgagttgctc acttgggcgc
     2641 tatgccgtgt gagttgcatc attgccggcc acgatgctca tgaccgccat gcgcaccgca
     2701 atgccaaaag tcacttgcgg caggatcacg ctctgcctgc catcgaccac cgccgagtca
     2761 atttcgacac cgcggttgat cggccccgga tgcatcacga tggcatccgg cctggccagt
     2821 cgcaactttt cctgggtcag gccaaaactc ttgaaaaact cctggctcga cggcagcaag
     2881 gcaccgctca tgcgctcatt ttgcaggcgc agcatgatga ccacgtcgca gccccggatg
     2941 ccttcctcaa gcgtgtggca gacccgcacc cccatttgtg ccatgtcgga cggcaccagc
     3001 gtctttgggc ccaccacccg cacttcggcg cagcccagcg tggtcagggc gtgaatgtca
     3061 gagcgtgcca cccgtgaatg cagtacgtcc ccgacgatgg ctaccgtcag gttgctgaag
     3121 tcctgcttgt agtggcgaat cgtgtacgcg tcgagcagcc cttgtgtcgg gtgtgcgtgg
     3181 cgaccgtcac cggcgttgat cacatgcaca tgcggtgcca catgctgggc aatcagatag
     3241 ggcgcgccag attcactgtg gcgcacgaca aaaatatcag ccgccatggc gctcaggttg
     3301 gcaatggtgt caagcagcgt ctcgcctttg gaggccgagg atttggcgat gtcgaggttg
     3361 atcacatcgg cactcaggcg cttggcggcg atgtcaaaag tggtgcgggt gcgggtggag
     3421 ttctcaaaaa acaggttgaa gacacttttg ccacgcaaca gcggcacctt cttgacctcg
     3481 cggtcgctca ccgagacaaa gttggtggca gtgtcaagca cctgggtcag gatgctcttg
     3541 ggcagacctt cggtggacag caaatggatc agctcgccat ttttgttgag ctgcgggtta
     3601 cgtttgtaga gcatggtcaa tgcgctttca gattgaaact gaacttgtct gaggcatcgc
     3661 gcgccagcga cagcgactga ctggcgggca gatggatgcg ggcggcgcaa atgtctggct
     3721 gaatcggcag ctcgcggcca ccccggtcca ccagcaccgc cagaatgact ttggccgggc
     3781 ggccaaagtc atacagttcg ttgatgacgg cccgaatggt gcggccggta aacagcacgt
     3841 catcaagcag cagtatctgc gcattgttga catcaaacgg aattttggtc tgcgctgctg
     3901 cggtcatgcc gcgccgtgca aaatcgtcgc ggtgcatggc cgaggaaatc acgccgaccg
     3961 gctcggttag ccccaggtcc tgctgcagtc gctgggccag ccaggcaccg ccggaagtca
     4021 cccccagcag gcgcatgccg ggccgaaaga ttgcccgcac actgtgcacc aactcgctat
     4081 acaaggactc cgcgtccatc aacaaggcac cgacatcggg gctggcctgc gttgccacat
     4141 tactggttgt ttggcccggc cgactggcct gacccgactc gatctgactc atggcaaatt
     4201 ccttaaaaat tgttccaaaa tgatgcaggc cgcttcggca tcggcatcgc gcgcgccgtt
     4261 gccgatggcc tcggtcgtgg tgtagcgttc atcgacctca aacaccggca atttgaagcg
     4321 accatgcaac tggcgcgcaa accgccgggc ccgtgccgtg ttgtcatgcg cggcaccgtc
     4381 cgggtgaaaa ggcaccccga ccaccagcgc gtcgggctgc cattctttca gatggcgggc
     4441 aattttgaca aagcgggcat caccctcggc ggtg
//
LOCUS       DAOSLL010000402         4474 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17190_length_4474_cov_4.280380, whole genome shotgun sequence.
ACCESSION   DAOSLL010000402 DAOSLL010000000
VERSION     DAOSLL010000402.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17190_length_4474_cov_4.280380"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PLB25_15640"
     CDS             <1..236
                     /locus_tag="PLB25_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012748451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE43041.1"
                     /translation="AGTTENLDAITAVVLGGTSLFGGRGIILGTLVGAVIVGVFRNGL
                     TLMGVASVYQTLITGILVILAVAADQMSRKGAR"
     gene            236..1045
                     /locus_tag="PLB25_15645"
     CDS             236..1045
                     /locus_tag="PLB25_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE43042.1"
                     /translation="MATQASNLKNAPLVMQAKGIIKRFGQVTALDGSDFELRAGEIMA
                     VIGDNGAGKSSLIKVLSGANIPDEGEVYLDGKQVRFKSPIDARRAGIETVYQDLAVAP
                     AMSIAENLFLGREILRPGFIGQLLRLLDKKEMLAQAISRMNDLKVGIRSMTQAVETLS
                     GGQRQCVAVARAAAFAQHVVIMDEPTAALGVKEGNMVLELIRRVRDKGLPVILISHNM
                     PHVFEVADRIHIQRLGRRAAVVNPKTISMSDTVAVMTGAKTVEELPEIALA"
     gene            1055..1507
                     /locus_tag="PLB25_15650"
     CDS             1055..1507
                     /locus_tag="PLB25_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0048029 - monosaccharide binding [Evidence
                     IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU domain-containing protein"
                     /protein_id="HOE43043.1"
                     /translation="MLKGINPLLTPDLLKIMMEMGHDDALVLSDANFTANSIATGKPV
                     IRLPGASMLQVVEAISALLPLAADVRHPVAFMAVSGATDDYRSAQQREVIALITPHLL
                     PTQSVEPVERFAFYDRARAAYAIVVTGELQPFGNFILRKGVIGENLRP"
     gene            1504..2745
                     /locus_tag="PLB25_15655"
     CDS             1504..2745
                     /locus_tag="PLB25_15655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HOE43044.1"
                     /translation="MTPLRHAPAADFPALLRPRGSNHVGMRQFNERVVLQAIRLNGSL
                     PKADLARLTGLSAQTIGLITTRLEEDLLVIKHERLRGRIGQPSIPLALNPDGAFSIGI
                     KIGRRSADWLLVDFTGQVRQRQSLDYEFPDKTSLLPTIARHMNSMLDSLGPLRDRLVG
                     IGVAAPFQLGGWHRMLGLSEAQSETWNQINLGAEVQAMTEVPVSFAKDTSAACVAELV
                     VGRGRDLKNFLYLFVDTFVGGGLVINSHLHGGLHGNAGAVASLPMRLSRRISSDPTPP
                     DQLISHASLWELERRFLALGLDPHAAYDERALSEPYSEETQGWISQAATALAHTVVSG
                     TAFLDIDAVVIDGSFSRTLQTQLIEATQEALQHYNWEGLWHANIIAGNVGPDARALGG
                     ALMPLYANFAPDQDLFLKVGD"
     gene            complement(2822..3493)
                     /locus_tag="PLB25_15660"
     CDS             complement(2822..3493)
                     /locus_tag="PLB25_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOE43045.1"
                     /translation="MDIIMTLLDFILHVDKHLEVFVQTYGAWVYALLFLIIFVETGVV
                     VMPFLPGDSLLFVVGAMCGVGLMSYPLSVGLLLAAAILGNQSNYTIGRYFGPKVFGWE
                     DSRWFNKKAFEQAHTFYEKYGGITIVAARFMPFLRTFAPFVAGVAQMTRSRFTFFDVA
                     GGTLWVGGIVTVGFFFGNIPWVKLHLDKIIWAMILIPGLVVLWGAWQGRRKAALASRQ
                     EHKKM"
     gene            4200..>4474
                     /locus_tag="PLB25_15665"
     CDS             4200..>4474
                     /locus_tag="PLB25_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43046.1"
                     /translation="MCYWKQWRWPRTKIKNLLALGVSLKSAIEHGCSSNSCWQMSRTP
                     VINQAISNVWLREQGLLSVKDLWCKAQGYTGKKRKTSSSVPTWFFRPL"
BASE COUNT          912 a         1335 c         1364 g          863 t
ORIGIN      
        1 acgccggaac caccgaaaac ctggatgcca tcaccgccgt ggtgctcggc ggcaccagcc
       61 tgtttggcgg gcgcggcatc attctgggga ctttggtcgg tgcggtgatt gtgggtgtgt
      121 ttcgcaacgg cctcaccctg atgggtgtgg catcggtgta ccagaccctg atcaccggta
      181 ttttggtgat tctggcggtg gctgctgacc agatgtctcg caaaggagca aggtaatggc
      241 tacgcaggct tccaatctta aaaacgcccc gctggtgatg caggcgaagg gcatcatcaa
      301 acgctttggc caggtgaccg cgctcgatgg ctctgacttt gagctgcgcg ccggtgaaat
      361 catggccgtg attggcgaca acggtgccgg caagtcgtca ctgatcaagg tgctgtcggg
      421 tgccaacatc cccgacgaag gcgaggtcta tctggacggc aagcaagtgc gcttcaagtc
      481 gccgattgat gcccggcgcg ccggtatcga aacggtttat caggacctgg cagtagcccc
      541 ggccatgagc attgccgaaa acctgttttt gggtcgcgag atcctcaggc ccggcttcat
      601 cggccaactg ctgcgcctgc tggacaagaa agaaatgctg gcgcaggcga tttcacgcat
      661 gaacgacctg aaggtcggca tccgctcgat gacgcaggcg gtggaaacgc tctcgggagg
      721 tcagcgccag tgtgtggcgg tggcccgcgc ggccgcgttt gcgcagcacg tggtgatcat
      781 ggacgaaccg accgccgcac ttggcgtgaa ggaaggcaac atggtgctgg aactgattcg
      841 ccgggtgcgc gacaagggcc tgccggtgat tctgatcagc cacaacatgc cgcatgtgtt
      901 tgaggtagct gaccgcattc acatccagcg cctgggtcgc cgggcggccg tggtcaaccc
      961 caagaccatc agcatgagcg acacggtggc ggtgatgacc ggtgccaaga cggtcgaaga
     1021 gctgcctgaa attgcgctgg cctgaaagac ccacatgctc aaaggtatca accccctgct
     1081 cacccccgat ctgctgaaga tcatgatgga aatgggccac gacgacgcgc tggtgctcag
     1141 tgatgccaat ttcaccgcca acagcattgc caccggcaag ccggtaatcc gcctgcccgg
     1201 tgcgtccatg ctgcaagtgg tggaggccat cagcgcgctg ctgccactgg ctgccgatgt
     1261 ccggcacccg gtggccttca tggccgtcag tggtgcgact gacgactacc gctcggccca
     1321 gcagcgcgag gtgatcgcac tgatcacgcc acacctgctg ccgacgcaga gcgtcgagcc
     1381 agtcgagcgt tttgcgtttt acgaccgtgc ccgcgccgct tacgccatcg tggtcacggg
     1441 agaattgcag ccatttggca acttcattct gcgcaagggt gtgatcggcg aaaatctccg
     1501 tccatgacac cactccgaca tgcccccgct gctgatttcc cggcgctgtt gcgcccccgt
     1561 ggctccaacc acgtgggcat gcgccagttc aacgagcggg tggtgttgca ggcgattcgc
     1621 ctcaacggca gcctgcccaa agctgatctg gcgcggctga ccggcttgtc cgcccagacc
     1681 atcggcctga tcaccacccg gctggaagaa gatctgctgg tcatcaagca cgagcgcttg
     1741 cgcgggcgca ttggacagcc gtcgattcca ctggcactga accccgatgg ggctttttcg
     1801 atcggcatca agatcggtcg gcgcagtgcc gactggctgc tggtggactt caccgggcag
     1861 gtgcgccagc gccaaagcct ggactatgaa ttccccgaca aaaccagcct gctgccgacg
     1921 attgcccggc acatgaacag catgcttgat tcacttggcc ctttgagaga ccggctggtc
     1981 ggcatcggtg tggcggcacc gtttcagctc ggtggctggc accgcatgct gggcctgtcc
     2041 gaagcgcagt ccgaaacctg gaaccagatc aacctgggtg ccgaggtgca agccatgacc
     2101 gaggtgccag tgagctttgc caaagataca tcggcggcct gtgtggccga actggtggtt
     2161 gggcgtggcc gtgacctgaa aaacttcctg tacctgtttg tcgatacctt tgtcggtggc
     2221 ggcctggtca tcaactcgca tctgcatggt ggcctgcacg gcaacgccgg tgcggtcgca
     2281 tcgttgccga tgcggctgtc gcggcgcatc agttctgacc caaccccgcc agatcagctc
     2341 atcagccatg cctcattgtg ggagctggag cggcgctttc tggccctggg gctggaccca
     2401 catgcggcgt atgacgaacg tgcgctgagc gaaccctact cggaagaaac ccagggctgg
     2461 atcagccagg ctgccacggc actggcgcac acggtggtca gcggcacggc ttttctggac
     2521 attgacgcgg tggtcatcga cggctcgttc agccggaccc tgcagaccca actgattgaa
     2581 gccacccagg aagcacttca gcactacaac tgggaaggcc tgtggcacgc caacatcatc
     2641 gctggcaatg ttggtccgga tgcgcgcgcc ctgggtggcg cgctgatgcc gctgtacgcc
     2701 aactttgcac cggaccagga tctgtttttg aaggttggcg attaaccctg gaaggccggg
     2761 caactgcccg agtttacaag tgttttagac cgctagcctt tgtcggcatt gcgctggcag
     2821 ctcacatttt tttgtgttcc tggcggctcg ccaaggccgc tttgcgccga ccttgccagg
     2881 ctccccacag caccaccagg ccgggaatca gaatcatcgc ccagatgatc ttgtccagat
     2941 gcagcttgac ccaggggata ttgccaaaga aaaagcccac cgtgacgatg ccgccgaccc
     3001 acagcgttcc ccccgccaca tcaaaaaaag tgaatcggct gcgcgtcatt tgtgccacac
     3061 ccgccacaaa cggggcaaag gtgcgcaaaa acggcatgaa acgcgccgcc acaatggtga
     3121 tgccgccgta cttttcgtaa aaggtgtggg cctgctcaaa cgcctttttg ttgaaccagc
     3181 gcgagtcttc ccagccaaac accttgggac caaagtagcg ccctatcgtg taattcgact
     3241 ggttgcccaa aatggctgcg gccagcaaca agccaaccga cagcgggtag ctcatcaacc
     3301 cgaccccgca catggctccc accacaaaca gcaacgagtc gcccggcaag aacggcatga
     3361 caaccacccc ggtttcgaca aagatgatca gaaacagcag cgcgtaaacc caggcaccgt
     3421 aggtctgtac aaacacctcc aggtgcttgt ccacgtgcaa gatgaagtcc agaagggtca
     3481 tgatgatgtc catgggcgca gattatcccc cggcccattg cgcgaccctt tgcccggtta
     3541 ctggccggat ctgaagttgg ctgttgcact gaggtaccga ggatcgcagc cggacaagcc
     3601 ttgatgccgg ttcgcagccg atgcgccata gggtctggct tcatcacagg ctgtgcattt
     3661 cgacatcgga tttggaaccc agtgccaggc gcaaagtgtg ccgtctggcg ctgtcagaca
     3721 agcgattgag cttgtgccca ggcgctcacg ccgatgttcg acccgacgtt ctcggaatcg
     3781 agtttcggtt tccggccagg ccgcaatgca caccaggcga tcaggcaggt acagcgacac
     3841 gtgaagggtg gtagaagcat cgcagtggac atcgatccgg ccaagttctt tgacaccgtc
     3901 aaccatgacg tgctgatgaa cttgctggct cgctccattg ccgacaagcg accgctgcat
     3961 ttgatcgggc gctacctgcg cgccggggtg ttggttggag agcacattga acccggcgaa
     4021 gtgggcaccc cacaggatgg gccactctcg ccactgctgg caaacttccg gcaccggatc
     4081 aaggagctga cgggcagaag ctggggcaat accttcgggg gtggacggcg tatttttgca
     4141 tcagccagta tgaccggccg accccggaat tggacgagtg gatcagacgg cgtatgcgta
     4201 tgtgctactg gaaacagtgg cgttggccgc gcacgaagat caagaatttg ctggcgttgg
     4261 gcgtgagcct gaagtcggcg attgagcacg gatgcagtag caacagttgt tggcagatgt
     4321 ccagaacccc ggtgatcaac caggccattt ccaacgtgtg gttaagggag caaggtttgc
     4381 tcagtgtgaa ggacctgtgg tgcaaggcac agggttacac gggaaagaag aggaaaactt
     4441 cgtcgagcgt gccaacttgg ttcttccgcc cact
//
LOCUS       DAOSLL010000403         4462 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17237_length_4462_cov_3.392784, whole genome shotgun sequence.
ACCESSION   DAOSLL010000403 DAOSLL010000000
VERSION     DAOSLL010000403.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4462)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4462
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17237_length_4462_cov_3.392784"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3301
                     /locus_tag="PLB25_15670"
     CDS             <1..3301
                     /locus_tag="PLB25_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE43047.1"
                     /translation="VDMDLLTRGAGFGYIGSTITSLIYASFTFIFFALEAAVMAYALE
                     LALGIPPRWGYLICALVVIPLVTHGVSTISRLQVWTQPLWLVMLVVPFAYVLMRDPGA
                     FAGVVHYVGEKSASGRFSLPLFGAALTVGVALMTQMGEQVDYLRFMPAQTPATRWRWW
                     LSVLAGGPGWVLLGVLKMLGGALLAYLAIQNAVPLDRAVDPNQMYLAAYEYVFPDYGW
                     AVAATALFVVLSQLKINVTNAYAGSLAWSNFFSRLTHSHPGRVVWVVFNTLIAFVLME
                     LNVFAALGDVLGLYSNLAIAWMMAVVADLVINKPLGLSPSGIEFKRAHLYDINPVGVG
                     AMALASLLSIAAYLGVFGPLAQAFSALIALGTALIASPLIAILTRSKYYLARADTPCD
                     STRCAGCKDGHARPADIQSGGADQPAPPGGEVSPHAGSYQRLKVQQCVICQRDYEAPD
                     MAHCPAYQGAICSLCCTLDARCGDLCKPHASLPRQWSAVLRWLLPKPTWALLDKGLGT
                     FVLLMLVIAPLLASVLGLLYHQESRALGQSIFDTQTLQTALDVLRNGFFNVYMALILI
                     AGLVAWWVVLAHQSRRVAQEESNRQTSLLLREIELHRQTDQALQQARQVAEQARAAAD
                     QANHAKSRYISAISHELRTPLNSILGYAQLMGEDTAIPAHRKQAVNVIKRGGEHLLSL
                     IEGTLDIAHIESGRLSLNVQPMQFAEFVSDIASLFELEAQAKGLTFRCDISNALPSVV
                     RADEKRVRQILINLLGNALKFTTQGHVVLRLRHVRDMGHIEIEDTGPGFSPQERARIF
                     EPFVRGESGNRQTGAGLGLTIAKMLTDLMGGDLSVDSVPGQGSVFKVKLFLPEVRMPP
                     SSSVSTVYSKASRHQNRLFVRRPRLGYVGPRRRILVVDNEEADRDLLIHLLQPIGFEV
                     RTAASGHDCLDLLAAGYQPEVILMDLAMPGIDGWETIRRLRVLLQGSPHAAPHIAIVS
                     ANAFDRGLDNDLGIPTEDFILKPVRHVDLLDWLERRLSLCWLETAVPAPVPPVPQVAG
                     EAVYPPAKQLDALRELVALGYYRGILNQLDEIERLSPDCAGFVQEMRALAKLFQFESI
                     SARLACIANEN"
     gene            3291..4277
                     /locus_tag="PLB25_15675"
     CDS             3291..4277
                     /locus_tag="PLB25_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE43048.1"
                     /translation="MKTSLPKPMLDRTLDRANSDVVLIVDDVPDNLAVLHDALDESGY
                     TVLVATHGQAALQRAAQVLPDIILLDAMMPGMDGFEVARQLKAMPKTAHIPIVFMTGL
                     TETEYLVAALESGGVDYVTKPIKPKEVLARMAVHLQGAREKRQARSALDAFGYASITV
                     RVSDGRLMWQTPLARDLLQRYYGTDAPTTPIPVLTWLRRHVGSTETLTEPPRLSIELS
                     SKRLTFRLHQQIGDSEGGGDWLIIMQEVSDESVIEAMALCFKLTPREAEVLYWVVKGK
                     INRDIGDIVGSSPMTVKKHLERVFVKLGVETRTAAASMAMSRIRQLHPQFEE"
BASE COUNT          794 a         1314 c         1410 g          944 t
ORIGIN      
        1 ggttgatatg gacctgctga ccaggggtgc gggttttggc tacatcggtt cgaccatcac
       61 ctcgctgatt tacgcctcgt tcacgttcat tttttttgcg ctggaagccg ccgtgatggc
      121 ttatgcactg gaactcgcgc tgggcatacc gccgcgctgg ggctatctga tctgtgcact
      181 ggtggtgatt ccgctggtca cccatggtgt ttcaaccatc agtcggctgc aggtctggac
      241 gcagccgctg tggctggtga tgctggtggt gccatttgcc tatgtattga tgcgtgaccc
      301 cggcgcgttc gccggtgttg tgcactatgt tggtgaaaaa tcggcctcgg gccggttcag
      361 tctgcctttg tttggtgcag cgctgaccgt tggcgtggcc ttgatgacgc aaatgggcga
      421 acaggtggac tacctgcgct tcatgccagc gcagaccccg gcgacccgct ggcgctggtg
      481 gttaagtgtg cttgccggtg gcccgggctg ggtgttgctg ggggtgctga agatgctcgg
      541 tggcgcgctg ctggcctacc tggcgattca aaacgcggtg ccgcttgacc gcgccgttga
      601 cccgaaccag atgtacttgg cggcttacga atatgtgttt cccgactatg gttgggcggt
      661 cgccgcgacc gccttgttcg tggtgttgtc acagctcaag atcaacgtca ccaacgccta
      721 tgcaggctcg ctggcctgga gcaatttttt ctcgcgcctg acgcacagcc acccaggccg
      781 ggtggtgtgg gtggtgttca acaccctgat cgcatttgtg ctgatggaac tcaatgtgtt
      841 tgccgcgctt ggcgacgtgc tgggcctgta ttccaacctg gcgattgcct ggatgatggc
      901 ggtggtggct gatctggtca tcaacaagcc gcttggcctg tcgccgtcgg gcatagagtt
      961 caagcgggcg catttgtatg acatcaaccc ggttggtgtc ggtgcgatgg ccctggcctc
     1021 cctgctgtca attgccgcgt atctcggcgt gtttggccct ctggcacagg cgttttcggc
     1081 gctgatcgcc ttgggtactg cgttgatagc ttcacctttg atagcaatat tgacgcgcag
     1141 caaatactat ctggcacgcg ccgacacgcc ctgtgacagt acgcgctgtg ctggctgcaa
     1201 agacggccat gcgcggccag cagatattca atcgggcggt gctgatcagc cagcaccgcc
     1261 aggtggtgaa gtttcgccgc atgcgggcag ttatcagcgt ctgaaggtgc agcaatgcgt
     1321 gatttgccag cgcgactacg aggcccccga catggcgcac tgcccagcct accagggtgc
     1381 catttgctcg ctgtgctgca cgcttgatgc gcgttgtggc gacctttgca aaccccacgc
     1441 cagtctgccg cgccaatggt ccgctgtgtt gcgctggctg ctgcccaagc cgacctgggc
     1501 gctgctggac aaaggcttgg gcacttttgt gctgctgatg ctggtcattg cgcctttgct
     1561 ggccagcgtg ctgggtttgt tgtaccacca ggaatcacgg gcgctgggcc agtcgatctt
     1621 tgatacccag acgctgcaga ccgcgctcga tgtcttgcgc aacggctttt ttaacgtcta
     1681 tatggcactt atcctgattg ccgggctggt cgcctggtgg gtggtgctgg cgcaccaaag
     1741 tcggcgcgtg gcgcaagagg aatcaaaccg gcaaaccagc ctgctgttgc gcgaaattga
     1801 attgcaccgg cagaccgacc aggcgctgca gcaggcccgg caggttgcag agcaggcgcg
     1861 ggcggcggca gaccaggcca accacgccaa aagccgctac atcagcgcca tcagccacga
     1921 gctgcgcacg ccgctcaaca gcatcttggg ctatgcacag ctcatgggcg aagacaccgc
     1981 catcccggcg caccgcaagc aggcggtgaa tgtcatcaag cgtggtggtg agcatttgct
     2041 gtcgttgatc gagggcacgc tcgacattgc ccacattgag agcggtcggc ttagcctgaa
     2101 cgtgcagcca atgcagtttg ccgaatttgt cagcgacatt gccagcctgt ttgagctgga
     2161 ggctcaggcc aaagggctga ccttccgctg tgacatcagc aacgcattgc cttcggtggt
     2221 tcgggccgac gagaagcggg tgcgccagat cctgatcaac ctgctgggca atgcgctcaa
     2281 attcaccacc cagggacacg tggtgctgcg gctgcggcat gtgcgcgaca tggggcacat
     2341 cgagatcgaa gacaccggtc cgggcttttc gccgcaggaa cgtgcgcgta ttttcgagcc
     2401 ctttgtccgt ggcgaatccg gcaaccggca gaccggggct ggtctgggcc tgacgatagc
     2461 caaaatgctc accgatctga tgggcggtga tttgtcggtg gacagcgtgc cagggcaagg
     2521 atcggtgttc aaggtcaagc tgtttttgcc cgaggttcgg atgccgccaa gctcatcggt
     2581 atcaaccgtg tactcaaagg cttcgcggca ccagaaccgg ctgtttgtcc gccgcccacg
     2641 gcttggctac gtcgggccgc gccgacgcat tctggtggtt gacaacgaag aggccgaccg
     2701 cgacctgctg atacaccttc tgcagccgat tggctttgag gtgcgcaccg ctgccagcgg
     2761 ccatgactgc ctggacctgc tggcggccgg ctaccagccc gaagtcatcc tgatggacct
     2821 ggccatgccg ggcattgacg gctgggagac cattcgacgg ctgcgcgtgc tgctccaagg
     2881 cagcccgcat gcggcgccgc acattgccat tgtgtcggcc aatgcctttg accgcggact
     2941 cgacaacgac ctgggtattc cgaccgaaga cttcattttg aagccggttc gccacgtgga
     3001 tttgctcgac tggctggagc gccgcttgtc gctgtgctgg ctggagacag ccgtcccggc
     3061 cccggtgccg cccgtgccgc aggtggctgg tgaggcggtt tacccgcctg ccaaacagct
     3121 tgacgcgttg cgtgaacttg tcgcgctggg ctactaccgg ggcattttga accagttgga
     3181 cgagatcgaa cggctgagcc cggactgcgc tggctttgtg caggaaatgc gcgcgctggc
     3241 caagctgttc cagtttgagt cgatcagtgc caggctggcc tgcattgcca atgaaaacta
     3301 gcctgcccaa gccgatgctc gaccgcaccc tggaccgcgc caacagcgac gtggtgctaa
     3361 tcgttgatga cgtgcccgac aacctggcgg tgctgcacga cgcactcgac gagtcaggtt
     3421 acaccgtgct ggtcgccacg cacggccagg cggccttgca gcgcgctgcg caagtgctgc
     3481 ccgacatcat cttgctcgat gccatgatgc ccggcatgga cggatttgag gtggcccggc
     3541 agctcaaggc gatgccgaaa accgcccata ttccgattgt tttcatgaca ggtctgaccg
     3601 agaccgagta cctggtggcg gcgctggagt ctggcggcgt tgattacgtt accaagccga
     3661 tcaaacccaa ggaagtgctg gcgcgcatgg cagtgcattt gcagggggcg cgtgaaaagc
     3721 gccaggcccg cagcgccctg gatgcctttg gctatgccag catcacggtg cgggtcagcg
     3781 acggtcgcct gatgtggcaa accccgctgg cgcgcgacct gctgcagcgc tattacggca
     3841 ccgacgcacc gaccaccccg atcccggtgt tgacctggct gcgccgccat gtcggcagca
     3901 ccgagacgct gaccgagcca ccgcgcctga gcattgaact cagctccaag cggctgacct
     3961 ttcggctgca ccagcaaatt ggcgacagcg aaggtggcgg cgactggctc atcatcatgc
     4021 aggaggtatc ggacgagagc gtgattgaag ccatggcgct gtgcttcaaa ctcaccccgc
     4081 gcgaagccga ggtgctgtac tgggtggtca agggcaagat caaccgtgac attggcgaca
     4141 ttgttggcag tagcccgatg acggtcaaaa aacacctgga gcgggtgttt gtcaagctcg
     4201 gtgtcgaaac ccgcaccgcc gcagcctcga tggcaatgag ccgcatccgc caactgcacc
     4261 cccagtttga ggaatagcgc tggctggcca gattggccaa ggccggtcag cggcgacgcc
     4321 tggcggctgc tttacaagcc ttttaagatg aagttgcatc cggatttggt taaattccta
     4381 ttgaaatcaa cacgttagtg tcgttttgag tgcaagtatg tagacatgca cataaatatc
     4441 gtcccaaatc gtggctccgc cc
//
LOCUS       DAOSLL010000404         4461 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17240_length_4461_cov_4.183159, whole genome shotgun sequence.
ACCESSION   DAOSLL010000404 DAOSLL010000000
VERSION     DAOSLL010000404.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17240_length_4461_cov_4.183159"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="PLB25_15680"
     CDS             complement(<1..1183)
                     /locus_tag="PLB25_15680"
                     /inference="COORDINATES: protein motif:HMM:NF012722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE43049.1"
                     /translation="MSADATLFPEGYGWHFLATSASVAVVRVNATGTILATNLYAVNL
                     VGEPLVGKPWHTMLLNFGGTPVLGDWLAKPARPRLLNIKTATGLPQTLEITVEPDGAE
                     YLLFGEVNVKEQTRLSREVLVLNHELNNMSRELALKNDVLIKTQDQLVQSEKLASIGL
                     LAGGVAHEINNPLAVVKSNLGRLKQYVKTLLTVIHASDAIEADASGSTSTWAALQSLK
                     QESDLPFITNDVVLLLAECGEGLDRVEKIVRGLRDFSRMDVRETWAFEDIHQILESAL
                     TAVGPELTTRCVVVKEYGHIAPVQCMRSEICQVFMSLLVNAAQAMEDPGVMTICTGQE
                     KSEAWIEIADTGHGITQTDLPLIFDPFFTTRPVGQGTGLGLSLAWGIVQRHRGTIEVE
                     SQ"
     gene            complement(1173..1823)
                     /locus_tag="PLB25_15685"
     CDS             complement(1173..1823)
                     /locus_tag="PLB25_15685"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOE43050.1"
                     /translation="MNTAIDEATYTHYLAALVAGDRETCAKTVQALLNAGTGLKPLYV
                     GLLQRAQYQVGELWEQEKISVAVEHLSTAITQRMLSLIQAQTLQGARRNRSIIVACVA
                     DEFHQLGGRMVADFCELRGWRGYFLGANTSIADLLQLIAQHKPDLLGLSLSIYFNLPK
                     LVAALDAVTAAYPDLPILVGGQAFRWGAQDALQHYPNAAYMDSLDALEERMVAFER"
     gene            complement(1820..3124)
                     /locus_tag="PLB25_15690"
     CDS             complement(1820..3124)
                     /locus_tag="PLB25_15690"
                     /inference="COORDINATES: protein motif:HMM:NF013306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HOE43051.1"
                     /translation="MQAPLFGQQLTLMEELKAATWTAHARLQTAPFFQALAGCQLPLE
                     SYVGQLRALLAIHAVVEQAMADCDDDRVRLVWRADMRRLPLLRQDLLYFEPRTVADLK
                     EAVDVANHTTGALRQQSLETPLALLGWLYVLEGSTLGASVVQPLVARALLLVGDEGVA
                     YLHNDGAAARARWTEYSQRMNTLALETEERAEITLAANALFAQLEAIFCALYPFQNES
                     KAYLAASINPEAGRHPIPVDLREVQASIRAGDICWYRFPYFAARYGERGRRFARSDAA
                     WQATLYQYPPAQILQQVQWLGRVLAARGMPTLLLQVQLEILVEELAAVIPERRADYER
                     LLPAAAALHAARSQCLTDVQAQELAVAFDLAVGPEWSARFAHTGSLLTAAVADECGGS
                     EKAVDSLRTWMTDVRQFPASWIDAVESTLAQARQQAVPRVKA"
     gene            complement(3317..>4461)
                     /locus_tag="PLB25_15695"
     CDS             complement(3317..>4461)
                     /locus_tag="PLB25_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE43052.1"
                     /translation="AFMASFFADQEAYQRNLAAIHLDSDLESFDTLELTDGRIFERRS
                     RPARHAEQIIGRVFSFSDATERKVIEQQLLNQNARLEEVVQQRTAALSQQLAALTESN
                     RKLEEAQFQLLQSEKLASIGQLAAGVAHEINNPIGFINSNLGTLGGYVESLLAIDAAY
                     TQAQHALDPSVQPTFEAVNSLKTSLDYAFMVDDLRQLIKESRDGLERVKTIVRDLKDF
                     ARVGSAEWAWADIHQGLESTLNIVWNELKYKATVEREYGTLPQVHCIPSQLNQVFMNL
                     LVNAAHAIERQGVIRVRTGCNDQQVWVEVIDTGIGIPAENLNRIFDPFFTTKALGKGT
                     GLGLSLAWGIVQRHRGTIEVESQPGKGSTFRVMLPIEGQMEASVSK"
BASE COUNT          935 a         1328 c         1271 g          927 t
ORIGIN      
        1 gctgtgattc gacttcaatc gtgccacggt gccgctgcac aatgccccag gccagcgaca
       61 gccccagccc ggtgccttga cccacaggcc gggtcgtgaa gaaagggtca aagatgaggg
      121 gcaaatctgt ttgggtgatg ccatggcccg tgtcggcaat ctcaatccag gcctctgatt
      181 tctcttgccc ggtacaaatg gtcatcacgc cggggtcttc catggcttgc gcggcgttga
      241 ccagcaggct catgaagacc tggcatatct cactgcgcat gcactgcacg ggtgcgatgt
      301 ggccatattc cttgacgacc acacatcggg ttgtgagttc tggaccaacg gcggtgagcg
      361 cgctctccaa aatctggtga atgtcctcga acgcccaggt ttcccggaca tccatgcgcg
      421 aaaaatccct aagcccacgc acgatcttct ccacccgatc aaggccctca ccgcattctg
      481 cgaggagcaa taccacgtcg ttcgtgatga agggtaagtc gctctcctgc ttgagggatt
      541 gcagggcggc ccaagtcgag gttgaacctg aagcatcagc ttcaattgca tcagaggcat
      601 ggatgacggt aagaagcgtt ttgacgtatt gtttcaatcg gcccagattg gatttgacga
      661 ccgccaacgg gttgttgatt tcgtgggcaa cgccgccggc cagcagtcca atcgacgcca
      721 gtttttctga ctgcaccaat tggtcctggg tcttgatcag cacgtcgttc ttgagcgcga
      781 gttcgcggct catgttgttg agctcgtggt tgagtaccag cacctcgcgg cttagccgag
      841 tctgctcctt tacattcact tcgccaaaca ggaggtattc cgcgccatcg ggctctacgg
      901 tgatttccag cgtttgtggc aagccggtag cggtcttgat attcaaaagg cgcggtcgag
      961 caggcttggc gagccaatcg ccaagcacag gagtgccacc aaaattgagc agcatggtgt
     1021 gccagggctt tcccaccagt ggctcaccaa ccagattcac ggcatacagg ttggtggcga
     1081 gtatggttcc cgtcgcattg acccgcacca ccgcgacgct ggcactcgtc gccagaaaat
     1141 gccaaccgta gccctcgggg aacaaggtgg catcagcgct caaaggcgac catccgttct
     1201 tccaacgcat ccagtgaatc catgtaggcg gcgttggggt aatgctgcag cgcgtcctgc
     1261 gcaccccaac gaaaggcctg accgccgacc agaatgggca gatcagggta ggcggccgtg
     1321 accgcatcaa gcgctgcaac gagtttggga agattgaaat agatgctaag cgacaagccc
     1381 agcaaatcgg gcttgtgctg cgcgatcaat tgcagcaaat ctgcaatgga cgtgttggca
     1441 cccagaaagt acccacgcca gccgcgcaat tcgcaaaaat cggcaaccat ccgaccacca
     1501 agctggtgaa actcgtctgc cacacaggcg acgatgatgc tccggttgcg tcgtgcgcct
     1561 tgtagcgtct gtgcctgtat cagtgacagc atgcgctggg taatggcggt ggacaaatgc
     1621 tctacagcga ccgaaatctt ttcttgctcc caaagttcgc caacctggta ttgagcgcgc
     1681 tgcaatagcc caacgtacag cggcttgagc cccgtcccgg cgttcagcag cgcctgcacc
     1741 gtcttggcgc acgtttcgcg gtcgccggcc accagcgcgg caaggtaatg cgtgtaggtg
     1801 gcttcgtcaa tcgcggtgtt catgccttta ccctcggcac ggcctgctgt ctggcctgcg
     1861 ccagggtgct ctcgaccgca tcaatccagg aggcaggaaa ctgacgcacg tcggtcatcc
     1921 acgtgcgcaa actatcaact gccttctcgc ttcctccgca ttcgtcggcc accgccgcgg
     1981 ttagcaaaga cccggtgtgc gcaaaccgtg cgctccattc ggggccaacg gcaagatcaa
     2041 atgccacggc cagttcctgc gcttgcacat ccgtcaggca ctgcgagcgc gctgcatgca
     2101 gcgccgcagc ggcaggcagc agtctttcat aatctgccct gcgctcggga atcaccgcag
     2161 ccagctcctc gaccagaatt tccagttgaa cctgcaacaa cagggtgggc atgccacgtg
     2221 cggccaacac gcgacccaac cattgcacct gctgcaggat ttgtgctggt ggatactggt
     2281 agagcgtggc ttgccaggcg gcatcgctgc gggcaaagcg ccgcccccgt tcgccatagc
     2341 gcgccgcgaa atacggaaaa cggtaccagc agatatcacc tgcccgaatg gaggcctgca
     2401 cctcccgcag gtcaacggga atgggatgtc gtccggcttc cggattgatc gatgcagcaa
     2461 gataggcttt ggactcgttc tggaacgggt acaaagcgca aaaaatcgcc tcaagctggg
     2521 caaaaagtgc attggcagcc agcgtaattt ctgcgcgttc ttcggtttcc agtgcaagcg
     2581 tgttcatacg ctgcgaatac tcggtccatc gggcgcgggc cgccgctccg tcattgtgca
     2641 gataggccac tccttcatcg ccaaccagca acagggcgcg tgcaaccagg ggttgcacca
     2701 cggacgctcc cagggttgag ccctccagaa catacagcca acccaacagg gccagcggtg
     2761 tctccaagga ttgttgacgc agggctccgg ttgtgtggtt cgctacatcg acagcctcct
     2821 tgaggtcggc cacagtgcga ggctcaaagt acagcaggtc ttggcgcagc aagggcagcc
     2881 tacgcatgtc ggcacgccag accaaacgga ctctgtcatc atcacagtcc gccatcgcct
     2941 gctcaaccac ggcgtgtatt gccaacaaag cacgcagttg tcccacgtag gattccagcg
     3001 gcaattggca tcccgccaat gcctgaaaga aaggggccgt ttgcaaccgg gcatgcgcgg
     3061 tccatgtggc tgccttgagt tcctccatca aagtcaactg ctgtccaaac aggggggctt
     3121 gcatcgtggc ctttctacct tgcaaaggac aatcttgcgt tgccccagtg ctgcggccta
     3181 gtttagacga tgtgactggt ctgacagtaa agaattagtt agaggcttat gcatggaagc
     3241 agtcgccagc cctcgtagaa caagcgacga tagctacaac ataagtagca tttgctggtg
     3301 catgactggc tggtgtctat ttggacaccg atgcttccat ttgcccttcg atcggcagca
     3361 tcacacgaaa cgtggagcct ttgccgggct gtgattcgac ttcaatcgtg ccacggtgcc
     3421 gctgcacaat gccccaggcc agagacaacc ccaggccggt gcccttgccc agcgccttgg
     3481 tggtgaagaa cgggtcaaag atgcggttga ggttttccgc ggggatgccg atgccggtgt
     3541 caatcacctc cacccagacc tgctggtcat tgcacccggt gcgaacccga atcacaccct
     3601 gcctctcgat ggcgtgcgcg gcgttgacca gcaagttcat gaaaacctgg ttcaactgtg
     3661 acggaatgca gtgcacctgg ggcagcgtgc cgtactcgcg ctctaccgta gccttgtatt
     3721 tgagttcatt ccagacgatg ttcagcgtgc tttctagtcc ctgatgaatg tcggcccacg
     3781 cccattcagc cgaccccaca cgggcgaagt ctttcaagtc acgcacaatc gttttgaccc
     3841 gctccaggcc gtcgcgcgac tctttgataa gctggcgcag gtcatccacc atgaaggcat
     3901 agtccaggct ggtttttagc gaattcaccg cctcgaaggt cggctgcaca gagggatcca
     3961 aggcatgttg cgcttgggtg taggcagcgt caatggcgag caacgactcc acatagccgc
     4021 ccagtgtgcc caggttggag ttgatgaaac cgattggatt attgatctcg tgcgctaccc
     4081 cggcggccaa ctggccgatg gatgccagct tttcagattg cagcagttga aactgggcct
     4141 cttcgagctt gcggttggat tcggtcagag ccgccaattg ctggctcagc gcggccgtgc
     4201 gttgttgcac cacttcttct agccgggcgt tctggttgag tagctgctgc tcgatcactt
     4261 tgcgctcggt ggcgtcgctg aacgaaaaaa cccgcccaat gatttgctct gcatgacgtg
     4321 ccgggcgtga acggcgctca aaaatgcgac catctgtaag ctccagcgtg tcaaacgact
     4381 cgaggtcgga gtccaggtga atggctgcca ggttacgctg gtaggcctct tggtccgcaa
     4441 aaaaactggc catgaaggcg a
//
LOCUS       DAOSLL010000405         4438 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17353_length_4438_cov_7.194387, whole genome shotgun sequence.
ACCESSION   DAOSLL010000405 DAOSLL010000000
VERSION     DAOSLL010000405.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17353_length_4438_cov_7.194387"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /gene="acpS"
                     /locus_tag="PLB25_15700"
     CDS             complement(<1..402)
                     /gene="acpS"
                     /locus_tag="PLB25_15700"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HOE43053.1"
                     /translation="MIYGIGTDICDVRRIKASLARHGERFAKRILSDDEYATWVTRSQ
                     RWPERGVRYLATRFSAKEAFSKAIGLGLTMPMSWRSCEVGKLPSGKPVIVLHGALKTW
                     FDAKGLSAHISVTDETDYAASFCVVEFKDENG"
     gene            complement(455..1213)
                     /locus_tag="PLB25_15705"
     CDS             complement(455..1213)
                     /locus_tag="PLB25_15705"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HOE43054.1"
                     /translation="MHTKPTALSVNLNKVASIRNTRHLGIPSLTRAATLCLQAGANGI
                     TVHPRPDERHIRAADVPELAALMQAWPDREYNIEGNPFHNLMEVVGQLVAQKLPVHQV
                     TFVPDGHGQFTSDHGWVFPQDAARLAPLIEQAHAWGVRVSLFMDAETAPMAGAKAVGA
                     DRVELYTEPYAAAWGTAAQGSQLARFAAAAQAALDVGLGVNAGHDLNRDNLGIFIAQV
                     PGISEVSIGHALIADALELGYAATVRDYLRCIAP"
     gene            complement(1283..2032)
                     /gene="recO"
                     /locus_tag="PLB25_15710"
     CDS             complement(1283..2032)
                     /gene="recO"
                     /locus_tag="PLB25_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HOE43055.1"
                     /translation="MAVKRISDEPAYLLHRYDWSETSLILEVFTRHHGRLALVAKGVK
                     RPSSSFRPILLPLQPLHIAFGGDAEIKTLKAAEWQGGHVMPTGDALMSGYYLNELLLN
                     LLAREDPHPLLFDVYASVVEVIASEHGEVLESALRTFELLLLREIGLLPLLDAQTMTL
                     LALHPAERYSLVPEGGLRQASDLDRASLSGAQWTALQQVLGDAVPFTAILRACSGVAA
                     ELKPQLRTLLNYHCGVTSLRTRQMMIDLQSL"
     gene            2672..2965
                     /locus_tag="PLB25_15715"
     CDS             2672..2965
                     /locus_tag="PLB25_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HOE43056.1"
                     /translation="METVGLFEAKTHLSEYVARAEAGEEVIITRHHKPVAKIVPFVDQ
                     PAPVLAVAPKFVQRTFNMGVPLVDLTQAGKLAGEPDEQGFIALTARLARSAKP"
     gene            3186..3617
                     /locus_tag="PLB25_15720"
     CDS             3186..3617
                     /locus_tag="PLB25_15720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOE43057.1"
                     /translation="MKLPDTQVLIHSVNEASPCRASASHWLEQAFDSGLGVGFAWLTL
                     IGFVRISTQRALLPAPLTVADALGLVDDWLTHPSAKILQPTVRHADILARLLLLAGGA
                     GYLAQDAHLGALAIEHGAVVGTFDPDFKKFPGVKFDLLLAS"
     gene            3658..>4438
                     /locus_tag="PLB25_15725"
     CDS             3658..>4438
                     /locus_tag="PLB25_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HOE43058.1"
                     /translation="MTSIPTLTVADIRKTFLDFFASKGHTVVASSPLVPGNDPTLMFT
                     NSGMVQFKDVFLGTDRRSYVRAASVQACLRAGGKHNDLENVGYTARHHTFFEMLGNWS
                     FGDYFKRESLMWAWELLTVVYKLPPERLMATVYAEDDEAYDIWTKDIGLPPERVVRIG
                     DNKGGRYKSDNFWMMADTGPCGPCSEIFYDHGAHIPGGPPGSPDEDGDRFIEIWNNVF
                     MQFDMAEDGSVTPLPAPCVDTGMGLERLAAILQHVHSNYEID"
BASE COUNT          865 a         1346 c         1324 g          903 t
ORIGIN      
        1 gccgttttca tccttgaact cgacgacaca gaagctggct gcgtagtcgg tctcgtcggt
       61 cacactgatg tgggcgctga ggcctttggc atcaaaccag gtcttcaatg ccccatgcaa
      121 aacaatcacc ggcttgccgc tgggcagttt gcccacctca caggagcgcc agctcatcgg
      181 catggttaac cccagaccaa tcgccttgct aaaggcctcc ttggcgctga agcgggttgc
      241 gaggtagcgc acgccacgct ccggccagcg ctggctgcgc gtgacccagg tggcgtattc
      301 gtcgtccgac aaaatccttt tggcgaagcg ctcgccatgt cgcgccaggc tggccttgat
      361 gcggcgtaca tcgcaaatgt cggtgccaat accgtagatc atgacgacag cgcacctgtg
      421 ggggctggcg cgctcaaggg tcggccgcag gggttcatgg ggcaatgcaa cgcaggtagt
      481 cgcgcaccgt ggcggcgtag cccagttcca atgcatcggc aatcagcgcg tggccaatcg
      541 acacctcgct gatgcccggc acctgcgcga tgaagatgcc caggttgtcg cggttcaggt
      601 catggccagc attgacgccc aggccgacat ccagcgcggc ctgggcagcg gcggcaaaac
      661 gtgccagttg gctgccctgt gcggctgtgc cccaggctgc ggcataaggc tcggtgtaca
      721 actcaacccg gtccgcgccg acggctttgg caccagccat cggtgcagtt tccgcatcca
      781 tgaacagact gacccgcacc ccccaggcat gcgcttgttc aatcagcggc gccaggcgcg
      841 ctgcgtcttg cggaaaaacc cagccgtggt cgctggtgaa ctggccatgg ccgtccggca
      901 caaacgtcac ttgatgcacc ggcagctttt gcgccaccag ttgccccacc acctccatca
      961 aattgtgaaa cggattgcct tcaatgttgt actcccgatc cggccaggct tgcatcaagg
     1021 cagccagttc gggcacatcg gcggcgcgaa tatgccgctc atccggacgc ggatgcaccg
     1081 tgatgccgtt cgcaccggct tgcaggcaca aggtggcggc gcgcgtcagg ctcggaatac
     1141 cgaggtggcg cgtgttgcgg atggaagcca ccttgttgag gttgacggag agcgctgtcg
     1201 gtttggtatg cataaggtat cagccagatt gtcagggtga gtctgtcgcc ttcatgacaa
     1261 agcggcaggt gcgcggcagg ttctaaagag attgcaggtc aatcatcatt tgccgggtgc
     1321 gcaagctggt cacaccgcaa tggtaattca gcaaggtccg caactggggt ttaagttctg
     1381 ctgccacccc ggagcaggca cgcaggatgg cggtaaacgg taccgcgtcg cccagcacct
     1441 gctgcaaggc ggtccattgt gcgccactga ggctggcacg gtccaggtcg ctggcctgac
     1501 gcaagccgcc ttctggcacc agactgtaac gctctgccgg gtgcagtgcc agcagcgtca
     1561 tggtctgcgc atcgagtagc ggcagcaggc cgatttcacg cagcagcagc agttcgaacg
     1621 tgcgcagggc agattccagc acctcgccgt gttcactggc aatcacctcg accacgctgg
     1681 cataaacatc aaacaacagc gggtgcgggt cttcgcgtgc cagcaggttc aacaacagtt
     1741 cgttgaggta atagcctgac atcagcgcat cacccgtggg catgacatgg ccaccctgcc
     1801 attcggcggc cttcaaggtc ttgatttcgg catcgccacc aaaggcgatg tgcagcggct
     1861 gcagcggcag caaaatcggt cgaaagcttg agcttggccg cttgaccccc ttggctacca
     1921 gcgccagtcg cccatggtgg cgggtaaaca cctccagaat caggctggtt tcgctccagt
     1981 cataacgatg aagcaggtaa gcgggctcat ccgagatgcg tttgacagcc atccgcgcgg
     2041 cttaccgaat caacccggcg acacggtctg gggccggtaa aaaaaccttt gccctgccct
     2101 tcaacctgac accgatggac cgcctgaaga cgctggccag gtggcacact ctgcgtgggt
     2161 cggcggacaa atgatcggga cgcgagggcg taaccggcgg cgtggcaagt tcagtggatt
     2221 cggtcatggg tttcctgttc aacaaatcgg ggcaagcgcc gattttcctg catatgggaa
     2281 cttgtcggac tcaggatgtg gttgggatta gctccccgtt ttgcaccaag gagttgggat
     2341 gcacggtcag gcgcaagacg cgccgtgcaa ctggctaaac aagcgtcttg caacgacgct
     2401 gtgcgcccaa atccgccggt gcaaataggg gaagttattt ctgaccacat ccttggggcg
     2461 catcggctgc aaaccaatcg cagcggccca ttttgcaccg ccggtttgag ccacaactgc
     2521 tctattggcc tgcaaatccg gcatcactgg ctggggtcga ttctcgccgc tcggcccgtt
     2581 agtccgacag gctaccgggt tcaaccgaga aatccgggtc aaaccccatg cctgtaaaat
     2641 ctggttgatt caaccaacct ggagcgctac cgtggaaacc gtcggcctgt tcgaagccaa
     2701 aactcatttg agcgagtatg tggcgcgtgc cgaggcgggt gaagaagtca tcattacccg
     2761 gcatcacaag ccggtggcca aaattgtgcc gtttgttgac cagccagcgc cagtgttggc
     2821 cgtcgcgccc aagtttgtgc agcgcacctt caacatgggc gtgccactgg ttgatctgac
     2881 acaagctggc aaactggcgg gcgaaccaga tgaacagggt ttcatcgcgt tgaccgccag
     2941 gctggcgcgc tcggccaaac cgtgattccg cgctggcccg gatgcacctg gattcctccg
     3001 ctgaccgctt gtcttcatct gtaagccatt atgcgcattt acttttttag tttggatggg
     3061 gttcaccgat cgggtcaggg cgctgcgcac ccgatcgagc cactggcatt tggagctgcg
     3121 tctttcgccc tgccttggcg cgccaccagc gactcgctcg ggcgcgtcca actgagcaaa
     3181 ccaggatgaa actccctgac acccaggtgc tgatccattc ggtcaacgaa gccagcccct
     3241 gccgcgcgtc ggcttcacat tggctggagc aggcctttga cagcggtttg ggggtcggtt
     3301 ttgcctggct gaccctgatc ggctttgtgc ggatatcgac ccaacgcgcc cttttgcctg
     3361 cgccgctgac ggtggcagat gccttgggac tggtggacga ctggctaacg cacccgagtg
     3421 ccaaaatcct tcagccgacg gtgcgccacg ccgacatttt ggcccggtta ctgctgctgg
     3481 ctggcggcgc tggatacctg gcccaggatg cccacctggg tgccctggcg attgagcacg
     3541 gggcggtggt cgggacgttt gaccccgatt tcaaaaaatt tcccggggtc aaatttgact
     3601 tgctgctagc ttcttgacca ttgtttttat tgccattttc gacatttcat tcctcttatg
     3661 acttccattc ccacgctcac tgttgccgac attcgcaaga cttttctcga cttttttgcc
     3721 tccaaaggcc acaccgtggt ggcatcgagc ccgctggtgc cgggcaacga tccgacgttg
     3781 atgttcacca actccggcat ggtgcagttc aaggacgtgt ttttgggcac cgaccggcgc
     3841 agttatgtgc gggcggcctc ggtacaggcc tgcctgcggg ctggtggcaa acacaatgac
     3901 ctggaaaacg tcggctacac cgcccgccat cacacctttt tcgagatgct gggcaactgg
     3961 agttttggcg actatttcaa gcgagagtcc ctgatgtggg cctgggaact gttgaccgtg
     4021 gtgtacaagc tgccgcctga gcgcctgatg gccaccgttt atgccgaaga cgacgaagcc
     4081 tatgacatct ggacaaaaga catcggtctg ccgcccgagc gcgtcgtgcg catcggcgac
     4141 aacaagggcg gtcgctacaa gagtgacaac ttctggatga tggccgacac cggcccctgc
     4201 ggcccgtgca gcgagatttt ttacgaccac ggcgcgcaca ttcctggtgg cccgccgggc
     4261 agtccggacg aagatggcga ccgctttatc gaaatctgga acaacgtgtt catgcagttc
     4321 gacatggccg aagacggctc ggtcacaccg ttgccagcac cctgcgtcga caccggcatg
     4381 ggtctggagc gcctcgccgc catcctgcaa cacgttcaca gcaattatga aatcgaca
//
LOCUS       DAOSLL010000406         4432 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17384_length_4432_cov_3.610921, whole genome shotgun sequence.
ACCESSION   DAOSLL010000406 DAOSLL010000000
VERSION     DAOSLL010000406.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17384_length_4432_cov_3.610921"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="PLB25_15730"
     CDS             complement(<1..1179)
                     /locus_tag="PLB25_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004629269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HOE43059.1"
                     /translation="MNPLNVEYRKVDALIPFARNPRTHSEEQVAKIAASIAEFGWTNP
                     VLVDGVSGVIAGHGRLAAARKLGLTEVPVIELGHLSPTQKRAYVIADNRLSLDAGWDE
                     EMLALELTELCESGYDLALTGFEDAEIEAMMAEHIDGETGDDPAGDDESDDAPDAGDD
                     VPDAPVNPVSRTGDVWLLGAHRLICGDAGDSAVVAALMQSEKAALCFTSPPYGTQRDY
                     TDTIVDWDALMRSVFANLPMAATGQVLVNLGLIHREQEVMPYWDGWLSWMRQQGWRRF
                     GWYVWDQGPGLPGDWSGRFAPAFEFVFHFNRKDSEVRRPNKNVPCIYAGRDTHLRGDG
                     TSAGGMRNKDGSKTSWNHVGTLTQDTKIADSVIRIMRHKGKIGQDIDHPAVFPVALPQ
                     F"
     gene            complement(1711..2109)
                     /locus_tag="PLB25_15735"
     CDS             complement(1711..2109)
                     /locus_tag="PLB25_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6362 family protein"
                     /protein_id="HOE43060.1"
                     /translation="MAERNTPWTPDDVANRFEDAAVTARRLPSANVQGYFNAWPTIVR
                     CQWEMLATDERVVCRFPPSPKDVEQMLEVMLWVQWLEVEQRHLVWMRAKRYGWRDICT
                     RFAIGRSTAWRHWNQAMALMAEKLNDSRKK"
     gene            complement(2102..2308)
                     /locus_tag="PLB25_15740"
     CDS             complement(2102..2308)
                     /locus_tag="PLB25_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43061.1"
                     /translation="MKVPTHHYQCPLGRLQPTVTDLDSVKQRGWRDQNILVVNAEDKR
                     LDYLERELVRRIGERLYGQGDRHG"
     gene            complement(2310..2792)
                     /locus_tag="PLB25_15745"
     CDS             complement(2310..2792)
                     /locus_tag="PLB25_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43062.1"
                     /translation="MQQTTISHTVTLALDLGTTTGWALKSRDSQIAHGFVSFKSQRFE
                     GGGMRFLRFKHWLAELKNMTGEINAVYFEEVRRHVGVDAAQVYGGLMATLTTWCEHHR
                     IPYQGVPVGTIKKHATGKGNAGKAEVIAAMRALGHPVTDDNEADALAILHWALDTQEV
                     "
     gene            complement(3019..>4432)
                     /locus_tag="PLB25_15750"
     CDS             complement(3019..>4432)
                     /locus_tag="PLB25_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012479842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HOE43063.1"
                     /translation="AVHLPDIGVNSHGNVPADRGGDHQDATVWGSEDALALSFTNRYQ
                     SDWRYVAAWGKWLMWDGQRWRSEDTLAASDLVRHVCRFASLKSDNPKLAAKLAASGTI
                     SGVERLARADRKHAGTTDEWDADEWSINTPGGVVDLRTGRMRAHDRGDRMTKICTATI
                     QPGSTCPNWLAFLADVTGGDAAQIHYLQKVFGYCMTGSTQEHALFFLYGTGANGKSVF
                     VNTIFTLMGDYAANAPMDTFMESRGDRHPTDLAGLRGARFVGATETEQGRRWNESKIK
                     EITGGDRVSARFMRQDFFTYLPQFKLVIAGNHKPAIRNIDEAMRRRLHLIPFTITVPP
                     EKRDKQLQAKLLLERNAIFEWGVQGCLAWQREGLIAPESVVSATKEYFEAEDALGRWM
                     EERCVRVPSAKSLTTELFSDWKQWAEAAGEFVGPQRRFSDLLLTRGLEKWRNSVGLRG
                     YQGIGLKEVRTPSYTPYADN"
BASE COUNT          865 a         1329 c         1314 g          924 t
ORIGIN      
        1 gaactgcggc agcgccaccg ggaacacggc cgggtggtca atgtcctgac cgatcttgcc
       61 cttgtgccgc atgatgcgaa tcaccgagtc agcaatcttg gtgtcctggg tgagcgtgcc
      121 gacgtggttc cacgatgtct tactgccatc tttgttgcgc atgccaccgg cgctggttcc
      181 gtcaccacgc agatgggtgt cacgtccagc gtagatgcag ggcacgttct tgttgggcct
      241 gcgcacctcg gagtccttgc gattgaagtg aaacacgaat tcaaacgctg gcgcaaagcg
      301 gccactccag tcgcctggca gacccgggcc ctggtcccaa acgtaccagc caaagcgccg
      361 ccacccctgc tggcgcatcc atgacagcca gccatcccag tacggcatga cttcctgctc
      421 gcggtggatc agcccaaggt tgaccagcac ctgaccggtg gcagccatgg gtaggtttgc
      481 gaagactgaa cgcatcagcg cgtcccagtc aacgattgtg tcggtgtagt cgcgttgggt
      541 accgtagggt ggtgaggtga aacacagcgc agccttttca gattgcatca gggccgcgac
      601 caccgccgag tcaccggcat cgccacagat cagacggtgc gcacctagca gccagacatc
      661 accggttcgg gataccgggt tgaccggcgc gtcaggcaca tcgtcaccag cgtctggggc
      721 atcgtcggat tcgtcatcgc ctgccgggtc gtcgccggtc tcgccatcaa tgtgttcggc
      781 catcatggct tcgatctcag catcttcaaa tccggtgagc gccaggtcgt aaccggactc
      841 acacagctcg gtcagttcaa gggccagcat ctcttcgtcc cacccggcat cgagtgacag
      901 gcggttgtcg gcaatcacgt atgcgcgctt ctgggtgggc gacaggtgcc ccagttcaat
      961 gaccggaact tccgtcaggc ccaacttgcg tgcagcagcc agacgaccgt gtccggcgat
     1021 cacgccgctg acaccgtcga ccagcaccgg atttgtccag ccaaactcgg cgatgctggc
     1081 ggcgatcttg gccacctgct cctcgctgtg ggtgcgagga ttgcgggcaa acgggatcag
     1141 cgcgtcaacc ttgcggtact caacgttgag gggattcacg ggtttcgagc tttccaaaaa
     1201 agtgcggccc gcacaggtct ataaaaccag tgacgggccg cgaggtcgct ttgagcaaag
     1261 cgcaggagtt gaacgagaga acccgacgtc agaaaactcc gagggcgggt tcaggaaaag
     1321 tcaggatacg aaaactcgga tgtcaggtcg gcaaagggtg tgaattgaat tccccccctt
     1381 gtgtgccagg gtgcaaacct gccgctggtg caaacccctg caaactctgg tttgcagtct
     1441 gtcggtgggc gggtcttgcg ctgttgcccc ccgcatagga ttttccgaaa gaaggacccc
     1501 ttttgcctgg ggcagagtgg ctaattacca actgattgcc agcggctttg tccatccata
     1561 ggcgtaaatg taccgaaaaa caggcaggat gtttcacctt catttgcagg caaaagggac
     1621 aaactccaaa tcaagggaca gagtgccaaa gcattaccct gcgtgaccca atagctcgga
     1681 tgatttgtcg agcaattggg tcttgccggg tcactttttg cggctgtcat tgagcttttc
     1741 tgccatcaac gccatggcct gattccaatg ccgccatgcg gtcgaccgtc cgatggcaaa
     1801 gcgcgtgcag atgtctcgcc agccgtaccg tttggcacgc atccagacca gatgacgctg
     1861 ctccacctca agccactgta cccagagcat gacctcaagc atctgctcca cgtccttcgg
     1921 cgatggcggg aagcgacaga ccacacgctc atcggttgcc agcatctccc actggcagcg
     1981 aacgatggta ggccacgcat tgaagtagcc ctgcacattg gctgagggca gtcgccttgc
     2041 agtgaccgca gcgtcctcga agcggttggc cacgtcatcg ggtgtccatg gtgtgttgcg
     2101 ctcagccatg acgatctcct tgaccataca aacgctcacc gatgcgtcgc accagttcac
     2161 gctcgaggta gtccagccgc ttgtcctcag cgttgaccac caggatgttc tggtcacgcc
     2221 agccgcgttg cttaacgctg tccaggtccg tgacggttgg ttgcaagcga ccaagggggc
     2281 attggtagtg gtgggtggga accttcacgt cacacctcct gcgtgtcgag tgcccagtgc
     2341 aggatggcca gggcatcggc ttcgttgtcg tccgtgacgg ggtgaccaag ggcacgcatg
     2401 gcagcaatga cttcagcctt accagcgttg cctttgccgg tggcgtgctt cttgatcgtg
     2461 cccacgggca cgccttggta cgggatgcgg tggtgttcgc accaagtggt cagtgtggcc
     2521 atcaagccac cgtagacttg ggcagcatcg actccaacgt ggcggcgcac ctcctcgaag
     2581 tagacagcgt tgatttcgcc agtcatgttt ttgagttctg ccagccaatg cttgaagcgc
     2641 aggaagcgca tgccaccgcc ttcaaagcgc tgggacttga agctcacgaa gccgtgagca
     2701 atctggctat ctcgggattt gagagcccag cctgtggtcg taccgaggtc aagcgccagg
     2761 gtgacagtgt gtgaaatggt ggtttgttgc atggggattg ggttcaaaag tgggtgttct
     2821 tggccggact gacgcagccg acgttgctca cggttaactt ccataacctg cgcgtcatgc
     2881 gcacgcgtga gaaactaacc gtaggcaaag tcggctgcgt cagaatcggg tttgactgac
     2941 acagccgaca cgcattgcca gattcgttct ttgcgtcagc agcgtcagtt ttggggtgga
     3001 ttgcgggcat ttgggttctc agttgtccgc ataaggcgtg taacttgggg tgcggacctc
     3061 tttcaaaccg atgccttgat aaccgcgcag gcccacggag ttgcgccatt tctcaaggcc
     3121 tcgggtgagc agcaaatccg agaagcggcg ttgtggcccg acaaattcac cagccgcctc
     3181 tgcccactgc ttccagtcgg agaacagctc ggtcgtcagt gatttggcgc tgggcacccg
     3241 cacacaacgt tcttccatcc agcgacccag ggcatcttct gcctcgaaat actccttggt
     3301 cgcactgacg acactctctg gcgcaatgag cccttcgcgt tgccaggcca gacaaccctg
     3361 tacgccccac tcaaagatcg cgttgcgctc gagcagaagc ttggcctgga gctgcttgtc
     3421 acgtttttca ggcggcacgg tgatcgtgaa cgggatcagg tgcaaacgcc tgcgcatggc
     3481 ttcatcgata ttgcgaatgg ccggtttgtg attgcctgcg atgaccagtt tgaactgcgg
     3541 caagtaggtg aagaagtcct ggcgcatgaa gcgcgccgac acccggtcgc caccggtgat
     3601 ctctttgatc ttcgattcgt tccagcgccg accctgttct gtctcggtcg caccgacaaa
     3661 gcgcgcccca cgcaggcctg ccagatcggt tgggtgccgg tcaccacggg attccatgaa
     3721 cgtgtccatg ggcgcgttgg ccgcatagtc acccatcagc gtgaagatgg tgttgacgaa
     3781 cacggacttg ccattggcac cggtgccata cagaaagaac agcgcgtgct cctgggtgga
     3841 gcctgtcatg cagtagccga acaccttttg caggtagtgg atctgcgccg cgtcaccacc
     3901 ggtcacatcg gccagaaacg ccagccagtt gggacaggtg ctgcccggct gaatcgtggc
     3961 ggtgcagatt ttggtcatgc ggtcaccacg atcatgggct cgcatcctgc cggtacgcag
     4021 gtcaaccacg ccaccgggcg tgttgattga ccactcgtcg gcatcccatt cgtcggtggt
     4081 gcccgcgtgc ttgcggtcag cccgagccag tcgctcgaca ccgctgatgg tgcccgaagc
     4141 tgccagtttg gccgcaagtt ttgggttgtc cgacttgagc gatgcgaaac gacagacgtg
     4201 gcgcaccagg tcggatgcgg ccagcgtgtc ttccgaacgc cagcgctgac catcccacat
     4261 cagccatttg ccccacgccg caacgtaacg ccagtcgctt tgataccggt tggtaaagct
     4321 cagtgccagc gcgtcctcac tgccccacac tgtcgcgtcc tggtggtcac caccccgatc
     4381 tgcgggtaca ttgccgtggc tattgacacc gatgtccggc agatgcaccg cc
//
LOCUS       DAOSLL010000407         4429 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17400_length_4429_cov_3.818473, whole genome shotgun sequence.
ACCESSION   DAOSLL010000407 DAOSLL010000000
VERSION     DAOSLL010000407.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17400_length_4429_cov_3.818473"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="PLB25_15755"
     CDS             complement(<1..658)
                     /locus_tag="PLB25_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43064.1"
                     /translation="MNGIRSNAPFFFSEKRGLEIANRQLKVLADSLEGVRTLALFNAA
                     GTMIASNRPEILNRNYADRAYFQVPLKEKNPSRLYVSAPIKTVLGVYSVVLSKSLVSA
                     DGEFAGIVTATLDPQDFKNTLEGAHDSPDSISAILHGNGQAFLFAPDAAALPASDTAK
                     MGDFFEDHKRSGLVTSVRTGFSPDTNAKRMMAMTTVYPPSLAMDYSLTISFSRDFDLF
                     F"
     gene            complement(852..2963)
                     /locus_tag="PLB25_15760"
     CDS             complement(852..2963)
                     /locus_tag="PLB25_15760"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE43065.1"
                     /translation="MDELQILRAWMHRSAIGLIVVDALGTIKFVNHAAQTLCACTVAQ
                     LLGHNCVALFSQDIAPADTLALQNSLVTAQAWSLQTMIQRMDGTQFWGNFQLVPVEDA
                     LAGSTHRCLFLNDISNLKHAADNIERLSSFDQLTLLPNRAQFLLNLHQLLSSAGDAGD
                     FVLMVWIDVDHLRAINDTMGQAVADKVLLTIADRLRDASRRQDLLGRLGGDEFALVIS
                     GGTNPQVLQDAAERLMRSLQTDLATPDCDVKFSLTAGTAIFPPDARYVEDLMSCAEMA
                     LLEAKKAGDGTIRHYVTGLGNTGTSHAQAVAELRTAIFSGELRLHYQPQVSLHSGQVI
                     GLEALVRWQHPSRGLLSPVHFIALAEECGLIIALGEWVMQTAVQQIKAWRDDGLTPVR
                     VAVNLSAPHFRQKGLPDAIEKLLFDHDVPASLFELELTESVMLRDAESAIRIVDRLKT
                     LGLRISLDDFGTGYSSLAYLSRFAIDVIKIDQSFIADITTNPVNASIATATIAMAHKL
                     GKTVIAEGVETLGQMVYLRRHECDEMQGYLFSRPVDTSAISDMLRSGADINLTLDQGE
                     TQMTLLLVDDEPNILNALKRLMRREGYRILCAESAAQGFDLLATHRVQVIISDQRMPV
                     MTGTEFLSRVKDLYPDTVRLVLSGYSELESLTDAINKGAIYRYISKPWEDDKFKQDVM
                     QAFRHYREHNPASTLNDHEAT"
     gene            complement(3023..>4429)
                     /locus_tag="PLB25_15765"
     CDS             complement(3023..>4429)
                     /locus_tag="PLB25_15765"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE43066.1"
                     /translation="LVVVVDHAIVDELARPGGPNMQAIGAVLDRLFDYAASHFKTEED
                     LMALHKLDARYVAHHCAIHAKFVEQVVGMRTLLSSDGALEVGPSLLRYLTSWLTIHIL
                     DEDRRFARHVALVEAGESASGAYDIVAKNNRSIDPARAALETALGDLYVVLGEHNKTL
                     QQANAKLQQARDQLEMRVHERTHELSAALEQMKLTQSQLLQSEKMAAVGQLAAGVAHE
                     INNPIGFVNSNLGSLKHYVAQLLNVISAYEAMETESSPLAERQQRLTQARAVADLDYM
                     KADVVNLLSESVDGLERVKKIVQDLKDFSRLDQAQWQDADINAGLESTLNVIAHELKY
                     RANVVKHFAPLPLLRCLPSQLNQVFMNLLLNAAQAFDGPGEINITTGFSDDNVWVDIR
                     DSGKGIPTDLQKRIFEPFFTTKPVGKGTGLGLTIAFDIVQKKHGGRIEVDSALGQGTT
                     FRIILPRHSAQPSPNNPG"
BASE COUNT          977 a         1232 c         1249 g          971 t
ORIGIN      
        1 caaaaaacag gtcaaagtcg cgactgaaag aaatggtcag ggaataatcc atcgccaggg
       61 atgggggata aaccgtggtc atggccatca tgcgcttagc attcgtgtcg gggctgaagc
      121 ctgttcttac gctcgtgacc aagccacttc tcttgtggtc ttcaaaaaaa tcccccatct
      181 tggcagtgtc gctagcgggg agcgcagcgg cgtcgggagc gaacaaaaag gcttgtccat
      241 tgccatgcaa aatagccgaa atcgagtccg gtgaatcatg cgccccctcc agtgtgtttt
      301 tgaagtcctg cgggtccagc gtagcagtca cgatacctgc aaattcgcca tcggcactca
      361 ccaggctttt tgacaacact accgagtaaa cccccagaac cgtcttgatt ggcgcgctga
      421 cgtaaaggcg ggatggattt ttttccttca gcggcacctg aaaataggct cggtctgcat
      481 agtttcgatt caaaatctcc gggcggttgg acgcgatcat cgttcctgcc gcattgaaca
      541 gtgccaaggt gcgaacgccc tccagggagt cagcaaggac tttcaactgc cggtttgcaa
      601 tttcaagccc acgcttttca gagaaaaaaa atggagcatt ggagcgaatg ccgttcatgg
      661 ccatgttgat tgacaccagg cgccgttgca ggtctgcggc agcagcagcc gagtgacggc
      721 ttagagaagc agcagcatca cttgcgatgc gcaggtaatc gaagtaaaga tcaacgccca
      781 acccagtgcc tacaaacagc agtgcgccgc ataggaggta ccacagcgaa cgtgaatgcg
      841 gcgtgggtac ttcaggtcgc ttcatggtcg ttcaatgtgc tcgccgggtt gtgctcgcgg
      901 tagtggcgaa aggcttgcat gacatcctgc ttgaacttgt cgtcttccca tggcttggag
      961 atgtagcggt agatggcgcc tttgttgatg gcatccgtga gcgattcgag ttcggaatag
     1021 cccgacagca ccagtcgcac cgtgtcgggg tacaggtctt tcacgcggct caaaaattcg
     1081 gtgcctgtca tgaccggcat gcgctggtcc gaaatgatga cctgcacgcg gtgcgttgcc
     1141 agcaagtcaa acccctgggc cgcactttca gcgcacagga tgcggtagcc ctcgcggcgc
     1201 ataagccgct tgagggcgtt cagaatattg ggctcgtcat ccaccagcag cagcgtcatt
     1261 tgcgtctcgc cctggtccag ggtcaaattg atgtccgcac cgctgcgcaa catgtcgctg
     1321 atcgctgagg tatccaccgg tcgggaaaac aggtaaccct gcatttcgtc gcactcatgg
     1381 cgccgcaggt ataccatttg ccccagggtc tccacccctt cggcgatcac agttttgccc
     1441 agtttgtggg ccatggcaat ggtggcagtg gcaatggacg cgttgaccgg gttggtggtg
     1501 atgtccgcga taaacgactg atcaatcttg atgacatcaa tggcaaagcg cgacaggtag
     1561 gccaaagacg aatatccggt accaaagtcg tccagagaaa tccgaaggcc cagtgtcttc
     1621 agccggtcca cgatgcgaat ggccgactca gcgtcgcgca acatcacact ttcggtcaac
     1681 tccagttcaa acaacgatgc cggaacgtca tggtcaaaca ataacttttc aatggcatcc
     1741 ggcagacctt tttgccgaaa atgcggggcc gacaggttga ctgccacccg caccggtgtc
     1801 aggccatcgt cccgccaggc cttgatttgc tgcacggccg tttgcatcac ccattcaccc
     1861 aacgcgatga tgaggccgca ctcttcggcc aacgcaatga aatgaacggg cgacagcagg
     1921 ccgcgcgagg ggtgctgcca gcgcaccagc gcttcaagcc caatgacctg gcccgagtgc
     1981 agactgacct gtggctggta atgcaggcgc agttcgccag aaaaaatggc agtgcgtagt
     2041 tctgcaaccg cctgtgcatg cgaggtgccc gtattgccaa gcccggtgac gtagtgtcgg
     2101 atggtgccgt caccggcttt cttggcctcc agcagtgcca tttctgcgca cgacatcagg
     2161 tcttcgacat atctggcatc aggcggaaaa atggcggtac cagcggtcaa gctaaatttc
     2221 acatcacaat ccggcgtggc aagatccgtt tgcaggctgc gcatcagtcg ctcagcagca
     2281 tcctgcaaaa cctgggggtt ggtgccgccg gatatcacca gtgcaaactc atcgccgcct
     2341 agtcgtccca gcaggtcctg gcggcgggag gcatcccgta ggcgatcagc aatggtcagc
     2401 agcaccttgt cggccacagc ttgccccatg gtgtcgttaa ttgctctaag gtggtcaacg
     2461 tcaatccaca ccatcaagac aaaatctccg gcatcgccgg cactgcttaa aagttggtgc
     2521 aggttcagca aaaattgggc gcggttaggc agcagcgtca attgatcaaa gctggacaag
     2581 cgttcaatgt tgtcagcagc gtgtttaagg ttgctgatat cgttcaggaa caaacaccgg
     2641 tgtgtggagc cagctagcgc gtcttctacc gggaccaact ggaagttgcc ccaaaattgt
     2701 gtgccgtcca tgcgttgaat catggtctgc agggaccaag cctgggccgt gaccaggctg
     2761 ttttgcaaag ccagggtatc ggcaggcgcg atgtcttggg aaaatagcgc tacacaattg
     2821 tgcccaagca gttgggcaac tgtgcaagcg caaagtgttt gcgccgcatg attcacaaat
     2881 ttgatggtgc ctagcgcatc taccacgatc agtccgatgg cggaccggtg catccatgcg
     2941 cgcaggatct gcagttcgtc cacagactgc ccaggttcgt ttgaatcagc gactgttgaa
     3001 ggaagtgtct caattgccat gctcaccccg gattgttcgg acttggttgc gcagaatggc
     3061 gcggcagaat gatgcgaaaa gtggtgccct gtcccagggc gctgtcgacc tcaatgcgac
     3121 cgccgtgctt cttttgcaca atatcaaatg caatggtcaa gcccagaccc gtgcccttgc
     3181 ctactggctt ggtcgtaaaa aatggctcga aaatccgctt ttgcaggtcc gtggggatgc
     3241 ctttgccgct gtcacggata tcaacccata cgttatcgtc cgagaaacca gtggtaatgt
     3301 tgatttcacc cggtccatcg aacgcctgcg ctgcattgag cagcaagttc ataaaaacct
     3361 ggttgagctg cgatggcagg cagcgcagca gcggcaatgg agcgaaatgc ttgaccacgt
     3421 tggcccggta cttgagttcg tgggcgatca cgttgagggt ggattccagc ccggcgttga
     3481 tgtcggcatc ctgccattgc gcctggtcca gacgtgagaa gtcttttaaa tcctgaacaa
     3541 tttttttaac ccgctccagg ccgtcgaccg actcgctcaa caggttcacc acgtctgcct
     3601 tcatgtagtc gaggtcggca actgcacgcg cttgggtcag gcgctgctgg cgctctgcca
     3661 gggggcttga ttcagtctcc atggcctcat acgcggatat cacattgagc agttgggcga
     3721 cgtagtgttt gagcgaaccg aggttggagt tgacaaagcc aatcgggttg ttaatttcat
     3781 gtgccacgcc agccgccaat tgtccgacgg ctgccatctt ttctgactgc agtaactggc
     3841 tttgtgtcaa tttcatttgc tccagcgctg ccgagagttc gtgggtgcgc tcgtgcaccc
     3901 tcatttcgag ctgatcacgc gcctgttgca gctttgcatt ggcctgctgc aaggtcttat
     3961 tgtgttcacc caacaccaca tacaggtcgc ccaacgcagt ttcaagcgcg gcacgggcgg
     4021 ggtcgataga acggttgttt ttggccacaa tgtcataggc gcctgaggcc gactcgccag
     4081 cctcaaccag cgcgacgtgt ctggcaaaac gccggtcttc atcaagaata tggatcgtca
     4141 gccaactggt caaatagcgt agcagcgacg ggccgacttc aagcgcacca tcgcttgaaa
     4201 gcagagttcg catgcccacg acctgctcca caaacttcgc atgaatggcg caatggtggg
     4261 caacgtacct tgcatcgagc ttgtgcaacg ccatcaggtc ttcttcggtc ttgaagtgac
     4321 tggctgcata gtcaaacagc cggtcgagta cggcaccgat ggcttgcatg ttcgggccgc
     4381 cgggtcgggc cagctcatcc acaatggcgt ggtccacaac tacgaccag
//
LOCUS       DAOSLL010000408         4420 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17440_length_4420_cov_5.774341, whole genome shotgun sequence.
ACCESSION   DAOSLL010000408 DAOSLL010000000
VERSION     DAOSLL010000408.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17440_length_4420_cov_5.774341"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            91..1047
                     /locus_tag="PLB25_15770"
     CDS             91..1047
                     /locus_tag="PLB25_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015768272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HOE43067.1"
                     /translation="MAMNRIQFQPGLSMPEFFSLFGTEALCASALESARWPDGFRCPD
                     CGGAAHCILHSIGNKVFQCNACRHQTSLIAGTVFQSTKLPLTIWFLAIYLVSQAKTGL
                     SALALKRQLGVSYPTAWLMQHKLMQAMTEREALYTLSGKVQVDDAYLGGERTGGKVGR
                     GSENKVAFVAAVSLTDEGHPLRVKLSPVPGFKLRAIADWAKKCLAAGSTVYSDGLACF
                     NAVTQAGCTHECTVVARRKPKDVPELQWINTVLGNLKTSLSGSYHSFGFRKYAAQYLG
                     TFAYRFNRRFNLKTLPQRLMVAAAQCGPHSQRSIRVVAEVHC"
     gene            1124..2140
                     /locus_tag="PLB25_15775"
     CDS             1124..2140
                     /locus_tag="PLB25_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43068.1"
                     /translation="MTIYRCNKCAFVSENTQVPIGAKANCERCSTPSMVFDTVYYVEK
                     LVERYRGALREIDELKVAAAAGLPAPQSAQSTLPPAPAGLMSVAEIDIHNTPVLATSA
                     QHQPLKDWFAAQQIEAIFDPSLVDTSGFFDDAARLIGRDFALFGELIDRIRYAYRNSH
                     SGVNIELASLSQKETQAITALCRQLYDHTFFSRYHYQKPEKIVRLGLQTAPAVRQFFD
                     GGWLEWFAFIEVMDQLKRQNKSFSCARGVKVVFANEDLHELDVIVLSEGQMPVCIECK
                     SGEFRRDIDKYLRLRKRLNLDRSRFIICATSLSDEQARGLNAMYELSFVNLASLPEKL
                     ATLL"
     gene            complement(2152..3582)
                     /locus_tag="PLB25_15780"
     CDS             complement(2152..3582)
                     /locus_tag="PLB25_15780"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HOE43069.1"
                     /translation="MTMDYWKRRWLARFDLRHDQADSAEIDATFRGGVEFRGTNLWVL
                     IFAIFIASIGLNVNSTAVIIGAMLISPLMGPIMALGYGAGINDSNLMRSSLFNLGLAT
                     VISLLTSTAYFLVTPLTQAHSELLARTTPSIWDVLIALFGGLAGVIGATRRVKSNLIP
                     GVAIATALMPPLCTAGYGLAIGNMAYFGGALYLYSINCVFIAIATLMMVRVMRMPSLT
                     QEQDQARIRHRTVIGLVVLTTLVPSVYLAINLVHQEWFDSNAARFVQQVLRSKPNVLV
                     VSQEPNFKGRQLRVNLVGDRLPEADIKAIEQRLPEFGLADVKLLLVQSGQVMPDMNTV
                     KRDLINELLQTNRADIAEREARIVALQGQLQAARQSAAAQLPLKDIYHEVRAQFPQAL
                     QVNVTAGFQGVMTKPDAPPDTAALDRPLLLVQLQLPTALGAAEQERLRLGLRQRAGLA
                     DLQAVRLEVSVAAAANPPANKRTSKR"
     gene            complement(3586..4359)
                     /gene="cysE"
                     /locus_tag="PLB25_15785"
     CDS             complement(3586..4359)
                     /gene="cysE"
                     /locus_tag="PLB25_15785"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HOE43070.1"
                     /translation="MFSRLRSDVQCILDRDPAARSTWEVLTCYPGLHAVLLHRVAHAC
                     WRRGFKWLGRYISHITRFLTGIEIHPGAVVGNRVFFDHAMGVVVGETAEIGDDCTIYQ
                     GVTLGGTSLYKGAKRHPTLGRGVVVGAGAQVLGGFNVGDGARVGSNAVVTKPVPAGAT
                     AVGNPARIILAELDVKREEAASKMGFSAYGVTQSDDPLSQAMRGLIDNASGHEHQIAM
                     LWKAIETLQANRPDADCVPGDAALNEHFEADRLNQLLGK"
BASE COUNT          911 a         1340 c         1292 g          877 t
ORIGIN      
        1 acttgattag caacaatctt cagccaagct cacctggtga gcttgtgggg gcgtaatctg
       61 cacccatctt ttagttttgg gggtcgcatc atggctatga atcgcatcca atttcaaccc
      121 ggcttgtcga tgccggagtt tttcagcctc tttggaaccg aggcgctttg cgcgtctgct
      181 ttggagagtg cacgttggcc cgatggtttt cgctgtcctg actgcggtgg tgctgcacac
      241 tgcatcctgc actccatcgg caacaaggtt tttcagtgca acgcctgtcg gcatcaaacc
      301 tcgctgatcg cgggcaccgt ctttcagtcg accaaattgc ctctgacgat atggttcttg
      361 gcgatttacc tcgtcagcca ggccaagacc ggtctgtctg ccctggctct caaacgtcag
      421 ctcggtgtga gctacccgac ggcgtggttg atgcagcaca aactgatgca agccatgact
      481 gagcgtgaag cactctacac gctcagtggc aaagtccagg tggatgatgc ctatctgggc
      541 ggcgagcgca caggtggcaa ggtcggacgc ggctccgaga acaaggtggc ctttgtggcg
      601 gcggtctccc tcactgatga gggccaccct ttgcgagtca aactcagccc ggtgcccggt
      661 ttcaagctca gagccattgc agactgggcc aaaaaatgcc tggccgctgg cagcaccgtc
      721 tattctgatg gcctggcctg cttcaacgcc gtgacccagg ctggctgcac ccatgaatgc
      781 actgtcgtgg caaggcgcaa acccaaagac gtacccgagt tgcagtggat caacacggtg
      841 ctgggaaacc tcaagaccag cctgagtggc agctaccaca gcttcggctt tagaaagtac
      901 gcagcgcaat acctcggcac cttcgcctac agattcaatc gcagattcaa tctcaagaca
      961 ctgccccaac gattgatggt ggccgcagcg cagtgcgggc cgcactcgca gcgctcgatt
     1021 cgggtggtgg ctgaagttca ttgctaatca agatagaata tggctccaga gcttatgaat
     1081 actgcgtaac aagctacgta gtcgatagcg ttcaggggtg cctatgacaa tttaccgatg
     1141 caacaaatgc gcttttgtct ctgaaaacac gcaggtgccg attggcgcaa aagccaactg
     1201 cgaacgctgc agcacaccga gcatggtgtt cgacacggtt tattacgtcg aaaaactggt
     1261 cgagcgttat cgaggtgcgc tgcgcgaaat tgacgagctt aaagtggctg cagcggctgg
     1321 cctgccagca ccgcaaagcg cccagtccac attgccgcca gcgcctgcag gcttgatgtc
     1381 ggtggcagaa attgacatcc acaacacccc ggtgctggcc accagcgcac agcaccagcc
     1441 gctgaaagac tggttcgccg cgcagcagat cgaagccatc ttcgatccgt ccctggtgga
     1501 caccagtggc ttttttgacg acgcagcacg cctgatcgga cgcgattttg ccctgtttgg
     1561 cgagttgatt gaccgcatcc ggtatgccta cagaaacagc cacagcgggg tcaatattga
     1621 actcgcatcg cttagccaaa aagagacgca ggccattacc gccttgtgcc gccaacttta
     1681 tgaccacact tttttctcac gctaccacta ccaaaagccc gagaaaattg tccggctggg
     1741 cttgcagaca gcgcctgcag tgcgtcagtt ttttgacggc ggctggcttg agtggtttgc
     1801 tttcatcgaa gtgatggacc agctcaagag acaaaacaag tcgttttcct gcgcgcgcgg
     1861 cgtcaaggtc gtgtttgcca acgaagacct gcatgaactc gatgtcatcg tgctgtccga
     1921 agggcaaatg ccggtctgca ttgaatgcaa gtcaggcgag tttcggcgtg acatcgacaa
     1981 gtacctgcgc ctgagaaagc ggctgaatct ggaccgcagc cgcttcatca tctgtgccac
     2041 cagcctgagc gacgaacagg cccggggctt gaatgcgatg tatgaactga gctttgtcaa
     2101 cctggcttcg ctgcctgaaa aactggcaac tttgctttga accaggattg ctcagcgttt
     2161 gctggtgcgc ttgttggctg gtggattggc cgctgctgcc actgaaacct ccagtcgcac
     2221 cgcttgcaga tcagcaagcc cggcgcgctg gcgcaggccc aggcgcagac gttcctgctc
     2281 ggcggcaccc agtgcggtgg gcaattgcag ttgcaccagc aacagcggcc gatccagggc
     2341 ggcggtgtcc ggcggcgcat ccggcttggt catcacgccc tgaaaaccgg cggtcacgtt
     2401 aacttgcaga gcctgtggaa actgcgccct gacctcgtgg tagatgtcct tgagcgggag
     2461 ctgtgctgcc gccgactggc gcgccgcctg caactgcccc tgcagcgcca caatgcgcgc
     2521 ctcgcgctcg gcaatgtcgg cacggttggt ttgcagcaac tcgttgatca ggtccctctt
     2581 gacggtgttc atgtccggca tgacttggcc cgactgcacc agcagcagct tgacatcggc
     2641 caggccaaac tccggcaggc gctgctcgat cgccttgatg tcggcctctg gcaggcggtc
     2701 gccgaccagg ttgacgcgca actgccggcc cttgaaattg ggctcttgcg acaccaccag
     2761 cacattgggc ttgctgcgca gcacctgttg cacaaagcgc gcggcattcg agtcaaacca
     2821 ctcctggtgc accagattga tcgccaggta cacgcttggc accagcgtcg tcaataccac
     2881 cagtccgatc accgtgcgat gccggatgcg cgcctggtcc tgttcctgcg tcaggcttgg
     2941 catgcgcatg acccgtacca tcatcagcgt ggcaatcgca atgaacacgc aattgatcga
     3001 gtacaggtag agcgcaccgc caaaataggc catgttgcca atcgccagcc cgtagcctgc
     3061 ggtgcacagc ggcggcatca gggccgtggc gatggcaaca ccgggaatca ggttggattt
     3121 gacccggcgg gtggcaccga tgaccccggc caggccgcca aacagcgcaa tcagcacatc
     3181 ccagatcgac ggcgtggtgc gcgccagcaa ttcggaatgg gcttgcgtca gcggcgtcac
     3241 cagaaaatag gcggtggagg tcagcaggct gatgacggtg gccagaccca ggttgaacag
     3301 cgatgaacgc atcaggttgg agtcgttgat gccggcaccg tagcccagcg ccatgatcgg
     3361 ccccatcagg ggcgatatca gcatggcacc gatgatgaca gcggtggaat tgacattcag
     3421 gccaatcgag gcgatgaaga tcgcaaagat cagcacccac aggttggtgc cgcgaaactc
     3481 gacaccaccg cgaaaggttg catcaatttc ggcgctgtcg gcctggtcat ggcgcaggtc
     3541 aaaacgcgcc agccagcggc gtttccaata gtccatcgtc atggcttatt tccccagcag
     3601 ttgattcagt cggtcggcct cgaaatgctc gttcaaggcg gcatctccgg gcacacaatc
     3661 ggcatccgga cggttggcct gcagcgtctc gatggctttc cacagcatgg caatctggtg
     3721 ttcgtgaccc gaagcgttgt caatcaggcc gcgcatcgcc tggctcagcg ggtcatcgct
     3781 ttgcgtcaca ccgtaggctg aaaaacccat tttggacgcc gcctcctcgc gtttcacatc
     3841 gagttcggcc aatatgatgc gtgccggatt gccgacggcc gtggcaccgg ctggcaccgg
     3901 cttggtcacc accgcgttgg agccgaccct ggccccgtca cccacgttaa agccgcccag
     3961 cacctgcgca ccggccccga ccaccacgcc gcgccccagc gtcgggtgcc gcttggcgcc
     4021 cttgtagagc gaggtaccgc ccagcgtgac accctggtaa atggtgcagt cgtcgccaat
     4081 ttcggcggtc tcaccgacca ccacgcccat cgcatggtca aaaaacaccc ggttgccgac
     4141 cactgcgccg gggtgaatct cgatgccggt caaaaagcgg gtgatgtgcg agatgtagcg
     4201 ccccagccat ttgaagccgc gccgccagca ggcgtgcgcc acccggtgca gcaacacggc
     4261 gtgcagaccg gggtagcagg tcagcacctc ccaggtgctg cgggcggccg ggtcgcggtc
     4321 aagaatgcat tgaacgtcgg atcgaagtct ggaaaacatg gtttaaccta agccggacga
     4381 gccggaattg gacggccttc gtcgcgaggg cgcgactcct
//
LOCUS       DAOSLL010000409         4411 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17486_length_4411_cov_4.239440, whole genome shotgun sequence.
ACCESSION   DAOSLL010000409 DAOSLL010000000
VERSION     DAOSLL010000409.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4411)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17486_length_4411_cov_4.239440"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..935)
                     /locus_tag="PLB25_15790"
     CDS             complement(240..935)
                     /locus_tag="PLB25_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE43071.1"
                     /translation="MLSIQNLSAGYGQSQVLQGMSLNIAPGEIVALLGRNGAGRSTLA
                     RAVVGLVAARGRIEWRGHDILGKKTFEIARLGLGYVPESRDVFRDLTVAQNLLLGLKP
                     GQPPGRWSIASMYQLFPQLALRSRVRAGVLSGGEQQMLTLCRTLMGDPALIIIDEPTE
                     GLAPQLVEAVADFLRALKAQGVSVLLIEQKLTMALAVSDRCAIMGHGRVVFEGTPEEL
                     QAAQAVRREWLEV"
     gene            complement(993..1583)
                     /locus_tag="PLB25_15795"
     CDS             complement(993..1583)
                     /locus_tag="PLB25_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOE43072.1"
                     /translation="MPNLSGRLAFILEAEKLKSVLRSAHTSTGRRESTAEHSWRLCLL
                     AMTLEDILGPLDFERMLKLCVIHDLGEAINGDVSAVLQQDSAPKSAQERQDFQKLTET
                     LPESLQAEFLSLWDDYEYARTREARIVKALDKIETIIQHNQGQNPADFDYAFNLQYGR
                     KYLAEHPVIAELRAMVDQATRLNAGTGQLSAPGRDA"
     gene            complement(1692..2216)
                     /locus_tag="PLB25_15800"
     CDS             complement(1692..2216)
                     /locus_tag="PLB25_15800"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011519099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HOE43073.1"
                     /translation="MWRVIGQIARAGICTEPAPDIGSDAQAQSARIQTELLNILGQAL
                     CIRQVDAGSCNGCELEINALGNPYYNIEGLGIKFVASPRHADLLLVTGPVSRNMEMAL
                     KRTYEATPEPKLVVAVGDCGCDGGIFGESYASCGRVANVVPVDVTVPGCPPEPLEILR
                     GILQAVRRRVKAKE"
     gene            complement(2216..3802)
                     /locus_tag="PLB25_15805"
     CDS             complement(2216..3802)
                     /locus_tag="PLB25_15805"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HOE43074.1"
                     /translation="MKIPGLDLEFVALAAPVPIWYAQVTGPQWLQAATTVRDEGGRLL
                     ALWASDATFSIAADAIDTRAGAVFVAYVRQGGVIWLALPLEIKNGCPVYPDLAPLFPV
                     AARMQRAGTDLSGIHAESAIDTRAWLNHGAWASDHFPLRQDPQALPQPLPGALTDYPF
                     VRVEGDGVHEIAVGPVHAGIIEPGHFRFSVVGERVLRLEQRLGYTHKGIEQRITQLAP
                     KEAHKLAGRVSGDSTVAYAWGYCMALESACQVTIPPRAAWLRALMLERERIANHLGDL
                     GAMGNDVAYAFGLAQFSRLREDWQRSSKAAFGHRFMMDCIVPGGVSGDISLEHKDQML
                     VHCQALELQVRRLFDIYEAHAGLQDRFMVTGRVTPELAAQLGLTGLAGRASGQARDLR
                     CDHPFAPYSELHVKMASHHRGDVGARAAVRFDEVFESIRLIRQMLGALPSGPVQCELT
                     LPAKPTKGAGWVEGWRGEVFVALELAGVDDGNRILRCHLHDPSWQNWPVLEHAIMHNI
                     VADFPLINKSFNLSYSGQDL"
     gene            complement(3807..>4411)
                     /locus_tag="PLB25_15810"
     CDS             complement(3807..>4411)
                     /locus_tag="PLB25_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004528370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HOE43075.1"
                     /translation="GLLSVVVAAFFLSRQKDVKRMFSYSSIEHMGLITFAFGMGSPVA
                     NFAGLLHMTVHSLVKSAIFFTVGHATQKAGTQLMSGITGLIKVNPTVGWGLMLGVMAI
                     LGMPPFGVFASEFLILTTAMREQPWATPFLFSGLGVAFASMLVRVLPMVFGDARLKPL
                     SHPPALVPVFVHLALALVLGLYIPPYLNGWYVQAAAMLGG"
BASE COUNT          907 a         1402 c         1283 g          819 t
ORIGIN      
        1 ccggccagca gcgcaccggc accttcaacc agcaagacct ttcgcattgc acgatttccc
       61 gcatggccga tgcggcagtg gcccatgacc agcacctggc gggcgcagcg cttgccacct
      121 tggccaatga ccggtgcgct gctgaatcaa cagttacctg aacaccggtc aggcacatca
      181 ttgccaaccc ggttgccagt ttttgcttcc caatcaggtc tgacggcacc ggatcaagtt
      241 cagacttcca gccactcgcg gcgaacagcc tgggctgcct gcaattcttc gggtgtgccc
      301 tcaaacacca cgcggccatg gcccatgatg gcgcagcggt ctgacactgc cagcgccatc
      361 gtcaacttct gttcgatcag caacaccgac accccttgcg ccttcagtgc cctgaggaaa
      421 tccgccaccg cctcaaccaa ttgcggtgcc agtccctcgg ttggctcgtc gatgatgatc
      481 agcgccgggt cgcccatcag ggtgcggcac agcgtcagca tttgctgttc gccgccggac
      541 agcacgccag cccgaacccg cgagcgcagc gcaagctggg gaaacaactg gtacatgctg
      601 gcaatcgacc agcggcccgg cggctggccg ggcttaagac ccagcagcag gttctgcgcc
      661 accgtcagat cccggaacac atcgcgactt tcgggcacat agcccaggcc caggcgggcg
      721 atctcaaatg ttttcttgcc cagaatgtcg tgtccgcgcc attcgatgcg gccgcgcgct
      781 gccaccagcc ccaccacagc cctggccagc gttgagcgac ccgcgccatt gcgccccagc
      841 agcgcgacaa tctcgccggg tgcaatgttc agcgacatgc cttgcagcac ctggctttgg
      901 ccatagccgg ccgacagatt ctggatgctc aacatttgcc gtgtggtccg atgaaaattc
      961 tgcagcgcat gtagcctgtc acgctgggca gttcaggcat cgcggcccgg tgccgaaagc
     1021 tgcccggtgc cagcgttcaa cctggtggct tgatcgacca tggcgcgaag ttctgctatg
     1081 actgggtgct cagcaaggta cttcctgcca tactgaagat tgaatgcgta gtcaaagtca
     1141 gccgggttct ggccctgatt gtgttgaatg atggtctcga tcttgtccaa tgccttgacg
     1201 atgcgagcct cgcgcgtcct ggcgtactca tagtcatccc agagcgacag aaactcggcc
     1261 tgcagtgact ccggcagggt ctcggtcagt ttctggaaat cctgcctttc ctgggcagat
     1321 ttcggcgctg aatcctgctg cagtacggcc gacacatcgc cgttgatcgc ttcacccagg
     1381 tcgtgaatga cacagagttt cagcatccgc tcaaaatcga gcggaccaag gatgtcctca
     1441 agcgtcatgg ccagcaggca aagccgccag ctatgctcgg ccgtgctttc ccggcgtccg
     1501 gtgctggtgt gggctgaacg cagcacgctt ttgagctttt ctgcttccaa gatgaaggcc
     1561 aggcggccgc tgaggttggg catgagtgca aagagtggat gggtacggcc aggtctttcg
     1621 gcttggctgg gtccatcaat gtctgaacaa tgccatttcc aaatcatcca tgccgatgtt
     1681 gatttgcagc ttcactcttt ggctttaacg cgcctgcgaa ctgcctgcaa gatgccacgc
     1741 aagatttcta gcggctcggg cgggcagccc ggcaccgtga catcgaccgg aaccacgttg
     1801 gcaacccggc cacaactggc ataactctcg ccgaagatgc cgccatcaca accacaatcg
     1861 cccaccgcaa ccaccagttt gggctcgggc gtggcttcat aggtgcgttt caaggccatt
     1921 tccatgttgc gcgacactgg cccggtgacc agcaacaggt cggcgtggcg cgggctggcg
     1981 acaaatttga tgccgaggcc ttcgatgttg tagtaggggt tgcccagcgc gttgatttca
     2041 agctcgcagc cgttgcatga accggcatca acctggcgaa tgcacaaagc ctgacccaga
     2101 atgttgagca gttcggtctg gatgcgcgca ctttgggcct gcgcatcgct gccaatatca
     2161 ggggctggct cggtgcaaat gccggcccgg gcgatctgtc caatgacgcg ccagatcata
     2221 aatcctgccc cgagtagctc aggttgaacg acttgttgat gagcggaaag tcggccacga
     2281 tgttgtgcat gatggcgtgt tccagcaccg gccagttttg ccatgacggg tcgtgcagat
     2341 ggcagcgcag gatgcggttg ccgtcatcga caccagcgag ctcaagcgcc acaaagacct
     2401 caccacgcca gccttcaacc caaccggcac ccttggtcgg tttggctggc aaggtcagct
     2461 cacattgcac cggcccgctc ggcagcgcac cgagcatctg gcggatcagg cggattgact
     2521 caaacacctc gtcaaaacgt accgcagccc gagccccgac atcgccacgg tggtggctgg
     2581 ccatcttgac atgcaactcg ctgtagggcg caaacggatg gtcacagcgc agatcccggg
     2641 cctggccgct ggcgcgaccg gccagcccgg tcaagcccaa ctgtgccgcc agttccgggg
     2701 tgactcgccc ggtcaccatg aagcggtcct gcaaaccggc atgtgcttca taaatgtcaa
     2761 aaagtcttct gacctgaagc tccagcgcct ggcagtgcac cagcatctgg tctttgtgct
     2821 ccaggctgat gtcgccagaa acaccgccag gcacgatgca atccatcatg aaacggtgac
     2881 caaacgcggc tttggagctg cgctgccagt cctcgcgcaa cctggaaaac tgcgccaggc
     2941 caaacgcata ggcaacatcg ttgcccatgg caccgaggtc gccgaggtgg ttggcgatgc
     3001 gttcgcgctc cagcatcaag gcacgcagcc aggcggcgcg cggcgggatg gtgacctggc
     3061 aggccgactc cagcgccatg cagtagcccc aggcataggc cacggtggag tcacccgaaa
     3121 ctcgcccggc caatttgtgc gcctctttgg gcgcgagttg cgtgatgcgc tgctcaatgc
     3181 ctttgtgggt gtagcccagg cgctgttcca gccgcaacac ccgttcgccg accaccgaaa
     3241 atctgaaatg cccaggctcg atgatgccgg catgcaccgg tccgactgca atctcgtgaa
     3301 cgccgtcgcc ctcgacccgc acgaaagggt agtcggtcag cgcgcctggc aggggttggg
     3361 gcagggcttg cgggtcttgc cgcagcggga aatggtcgct ggcccaggct ccgtggttca
     3421 accaggcgcg cgtgtcgatg gcactttctg cgtgaatgcc agacaggtcg gtgccagcgc
     3481 gctgcattct ggcggcaacc gggaacagcg gggccaggtc cgggtaaacc gggcatccgt
     3541 tcttgatctc cagcggcaat gccagccaga tcacgccacc ttgcctgaca tacgcaacaa
     3601 atacggctcc agcccttgtg tctattgcgt cagcagctat tgaaaaagta gcgtcagaag
     3661 cccacaatgc gagcagacga ccgccctcgt cgcgcaccgt ggtggcggct tgtagccact
     3721 gcggcccggt cacctgtgca taccagattg gcaccggtgc tgcgagcgcc acaaattcaa
     3781 ggtccagacc gggtatcttc attgcattag ccccccaaca tagctgcggc ctgaacgtac
     3841 cagccgttca gatagggcgg gatgtacaag cccagcacca gcgccagcgc caaatgcacg
     3901 aacaccggaa cgagcgccgg cggatggctt agcggcttga gtctggcatc gccaaacacc
     3961 atcggcagca cccgaaccag catcgaggca aacgccaccc ccaaccctga aaacagaaac
     4021 ggggtggccc agggttgctc gcgcatggcg gtggtcagga tcaggaattc gctggcaaac
     4081 acgccaaatg gcggcatgcc caaaatcgcc atcacgccca gcatcaggcc ccagccgacg
     4141 gtcgggttga ccttgatcag gccagtgatg ccgctcatca actgggtgcc ggccttttgc
     4201 gtggcatgtc cgacggtgaa gaaaatggct gacttgacca gtgagtgcac cgtcatgtgc
     4261 aacaagccgg caaaattggc caccggcgag cccatgccaa aagcaaaggt aatcaggccc
     4321 atgtgttcaa tgctggaata cgaaaacatg cgtttgacat ctttttgtcg cgatagaaaa
     4381 aacgccgcca ccaccacaga gagcaggcca a
//
LOCUS       DAOSLL010000410         4407 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17509_length_4407_cov_4.276425, whole genome shotgun sequence.
ACCESSION   DAOSLL010000410 DAOSLL010000000
VERSION     DAOSLL010000410.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4407)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17509_length_4407_cov_4.276425"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(49..222)
                     /locus_tag="PLB25_15815"
     CDS             complement(49..222)
                     /locus_tag="PLB25_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43076.1"
                     /translation="MKHFISAKLLATVALAIGAIAVASSAQARSEVHLSVGLQVPGVV
                     VKPMPVHVRPAPI"
     gene            586..1860
                     /locus_tag="PLB25_15820"
     CDS             586..1860
                     /locus_tag="PLB25_15820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOE43077.1"
                     /translation="MLDLLIQNARLPDGRSNMSVAVQDGKIVEVAAGLTAPTHRSVDA
                     QGFLLSPPFVDAHFHMDATLSYGLPRVNQSGTLLEGIALWGELKPLLTVDALIERALA
                     YCDWAVAKGLLAIRSHVDVCDERLLAVDALLQVKRQVAPYIKLQLVAFPQDGLLRAPT
                     ALANLKRALDKGVDVVGGIPHFERTSGQGAASVKLLCELAAQRGLPVDMHCDESDDPL
                     SRHIETLAFETQRLGLHGRVTGSHLTSMHSMDNYYVSKLIALIAEAQLQVVANPLINI
                     TLQGRHDSYPKRRGMTRVPELLAAGINVAFGHDCVMDPWYSLGSGDMLEVAQMGLHVA
                     QMTSQAAMRQCFDAVTVNAAGVMGLENYGLQAGCQADFVLLQARDPVEALRLRATRLK
                     VFGRGKLLAESPAAATALHLSGRPSQTGFALP"
     gene            2537..2965
                     /gene="mraZ"
                     /locus_tag="PLB25_15825"
     CDS             2537..2965
                     /gene="mraZ"
                     /locus_tag="PLB25_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HOE43078.1"
                     /translation="MFQGASSLSLDAKGRLSMPTRHRDVLTATASGQLTITKHPHGCL
                     MIFARPEWEKFRQRIAELPMSAQWWKRIFLGNAMDAELDGTGRVLVSPELRAAAGITK
                     DAVLLGMGSYFELWDKPTYDAQEAQAMQGDMPDVFRDFAF"
     gene            2975..3931
                     /gene="rsmH"
                     /locus_tag="PLB25_15830"
     CDS             2975..3931
                     /gene="rsmH"
                     /locus_tag="PLB25_15830"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HOE43079.1"
                     /translation="MGNSWTHTTVLLGEAVDALLGQSLEAQAHGMAARVYVDATFGRG
                     GHSRLILSRLMPTDRLIAFDKDTDAVDEAARIADTRFSIRHQGFSHLGELPVASVAGV
                     LLDLGISSPQVDNPARGFSFRFEGPLDMRMDTTRGQSVAQWLADAEFEHIAEVIREYG
                     EERYAGPIAKAIVARRQARGTLSTTTELAQLVADTVKTREPGQNPATRTFQAFRIFIN
                     AELEELQQGLGAALKVLQPGGRLVVISFHSLEDRIVKQFIASHSRHVFDRRAPFAVPQ
                     AMQLKALGRVRPGAAEVAANPRARSAIMRVAERNMIQAGAGT"
     gene            3928..4251
                     /gene="ftsL"
                     /locus_tag="PLB25_15835"
     CDS             3928..4251
                     /gene="ftsL"
                     /locus_tag="PLB25_15835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HOE43080.1"
                     /translation="MMRLNLLLAMGVVLSALYLVNVQYASRSLYAELDQARSAAHRLT
                     VESERLQVEKRAQSTSARVEQLARSRLQMRGANPAITAYVAYTAQVPAEPVAPRDPRK
                     QAGSP"
BASE COUNT          896 a         1241 c         1366 g          904 t
ORIGIN      
        1 tatttaagca gtaagaggtg gcgttttggt ggggtttccg ttctggcact agataggcgc
       61 ggggcgtacg tgcacaggca tcggttttac gacgacaccg ggtacctgca ggccgacaga
      121 aaggtgcacc tcgctgcggg cttgtgctga tgaagccaca gcgatggcac caattgcgag
      181 ggcaactgtc gcgagcaatt tggcggaaat gaagtgtttc atgaaagctc cttgggttgt
      241 tgaagaccac atcttgcccg cgcatgtatg aacgcaagct gaacagaatc aagaaaagtc
      301 ttgaagacca tggaaggcaa gccagcggcg gggcgaagtg cagatttgac ccgtgcgtca
      361 aatggcggtg catgcttggc tgaccccggt cgcgcgcatg ccaatgaaat ctcgcttcag
      421 tgccgcgtgg tatcaccagg cagcaatggc tgccggggtc ttgccggagc caatcgacgg
      481 cggtggttca acagtcatca gagtatgtca tcattttcgc tctcagccct tatagaatgt
      541 gtgctgacag ctatattact aatagcattt gaaaaggact gcgacatgct tgacctgttg
      601 attcaaaacg ccaggctgcc ggacgggcgc agcaacatgt cggtggccgt acaggacggc
      661 aaaatcgtgg aagtagccgc cggcctgaca gcgccaactc atcgaagcgt cgatgcgcag
      721 ggattcttgc tcagcccgcc gtttgtcgat gcacactttc acatggacgc cacactgagt
      781 tacgggctgc cacgggtcaa ccaatcgggc acgctgctgg agggcattgc gctgtggggt
      841 gaactcaagc cgctgctcac agtcgatgcg ctgatcgagc gcgcgctggc ctattgcgac
      901 tgggcggtag ccaaaggcct gctggccatt cgcagccatg tcgacgtgtg tgacgaacgc
      961 ctgctggcgg tcgatgcgct gctgcaagtc aaacgccagg tggcacccta tatcaagctg
     1021 caactggtgg cttttccgca agacggcctg ctgcgtgccc ccactgcgct ggcaaacctg
     1081 aaacgggcgc tggacaaagg ggtagatgtg gtgggtggca taccgcactt cgagcggact
     1141 tccgggcagg gcgcagccag tgtcaaactg ctgtgcgagc tggccgcgca gcgcggcctg
     1201 ccggtcgata tgcactgcga cgagtcggat gatccgctgt cgcgccacat tgagacactg
     1261 gcattcgaga cccagcgcct tggcctgcac gggcgtgtca ccgggtcgca cctgacttcc
     1321 atgcacagca tggacaacta ctatgtgagc aagctgatcg ccctgattgc agaggcgcag
     1381 ttgcaggtgg tggccaaccc gttgatcaac atcaccctgc agggtcggca cgacagttac
     1441 ccgaagcgcc ggggcatgac acgtgtgccc gagttgctgg cggccggcat caacgtcgcc
     1501 tttggccatg actgcgtcat ggacccctgg tacagcctgg gatcaggcga catgctcgaa
     1561 gtcgcccaga tgggtttgca tgtggcgcaa atgaccagcc aggccgccat gcgccagtgt
     1621 tttgacgcgg tcacggtcaa cgcagcaggt gtcatgggac tggaaaacta tggcctgcaa
     1681 gccggttgcc aggccgattt tgtgcttctg caagcgcgtg acccggtcga agcgctgcgg
     1741 ctgcgcgcga cccggctgaa ggtgtttggg cgcggcaagc tgttggccga gtcgcccgcc
     1801 gcagccacag cattgcacct gagcgggcgt cccagtcaga ccggctttgc cttgccgtag
     1861 cgctaatgcc gactttctgg cctggcggga attgatgctc aatgccgagg cgctgcaggg
     1921 caatgatggg tagcaggccc tgtcaacggc atcacctcac cggttggcaa gactgcggca
     1981 actgcatgcc acgcaatgac cggagatctg ccgtggtgag cccattgagg cgcagccggg
     2041 gccgggattg ttggctaaat gatgtggcgt gggtgaaagg gctgcgacag ttgttccgac
     2101 ctggcaagcg gatcgacatc gcctgtcgcg tcatcggtct agtcagggct caacgcatgc
     2161 agccgggagc cgatatgccg atgcacagca tttattggcc tgcgcccgcg gcaggttttg
     2221 atgtgggacc atcggcttgc gatctgtacc aagccaacgt tttgctgttt gcacgacgaa
     2281 cccagaatgc aaaccggtct gccaaatctt caagtattac cttcagaaca aactctaagt
     2341 ctttaatata aaaaggattt tttatcccaa ccttcaagtc ttggtctaag tcattgattt
     2401 cattgtaaaa aattgttgag cgcgagtttg caagcggcaa atttgctgat acagtgcaaa
     2461 aaagtgcaat ttagtggtga aaagtgccat ttcgccacct cttctgccaa aaaccaatca
     2521 ccaaaggtct gctgtcgtgt ttcaaggggc ttcatcattg agtctggatg caaaggggcg
     2581 gctgtcgatg ccgacccggc atcgtgatgt cttgaccgca accgccagtg gccaacttac
     2641 catcaccaag cacccgcacg gctgcctgat gatatttgcc cgccccgagt gggaaaagtt
     2701 ccgccaacgc atcgccgaat tgcccatgtc agcccagtgg tggaagcgga tatttctggg
     2761 taacgcgatg gatgctgaac ttgacggcac tggccgagtg ctggtgtcac ccgaactccg
     2821 tgcggcggca ggcatcacca aggatgccgt gttgctgggc atgggcagtt acttcgagtt
     2881 gtgggacaag cccacttacg acgcgcagga agcgcaggcc atgcaaggcg acatgcctga
     2941 tgtcttcagg gactttgctt tctgaaggac ggtggtgggt aattcatgga cacacaccac
     3001 tgtgttgttg ggtgaagcag ttgatgcttt acttggacaa tcacttgagg cacaggcgca
     3061 cggcatggca gcgcgcgtgt atgtcgatgc cacgttcgga cgtggtggtc atagtcgact
     3121 gattctgtcc agattgatgc cgaccgatcg cttgattgcc tttgacaagg ataccgatgc
     3181 agtggatgag gcggcgcgca tcgccgatac acggttctcc atccggcacc agggttttag
     3241 ccacttgggc gaactgccag tggccagtgt ggccggggtg ctgctggatt tgggtatcag
     3301 ttccccgcag gttgacaacc cggcgcgggg ctttagtttc aggttcgaag gacctctgga
     3361 catgcgcatg gacaccacgc ggggtcaaag cgtggcgcag tggctggccg acgcagagtt
     3421 tgaacacata gcggaggtaa tacgtgaata tggcgaagaa cggtatgctg gccccattgc
     3481 aaaggcgatt gttgcgcgcc gacaggctcg gggcaccctt tcaaccacca ctgaattggc
     3541 ccagctcgtg gctgacacgg tcaaaacccg cgagccgggc cagaaccctg caacgcgcac
     3601 atttcaggct tttcggattt tcatcaatgc cgagcttgaa gagttgcaac agggcttagg
     3661 cgcggccctc aaagtgctgc aaccgggcgg acggctggta gtgatcagct ttcactcgct
     3721 ggaagaccgc atcgtcaagc agttcattgc cagtcattcg cgtcatgttt ttgaccgccg
     3781 tgcgccgttt gccgtgccgc aggcgatgca actcaaggcc ctggggcgcg tccgtcctgg
     3841 tgctgccgag gtcgcggcca acccgcgcgc gcgcagcgcc atcatgcggg tggccgagcg
     3901 caacatgatc caagcggggg ccggcacatg atgcgactga acctgctgct ggcgatgggc
     3961 gtggtgttga gtgcactgta tctggtcaac gtgcaatacg cctcgcgcag cctctacgcc
     4021 gagcttgacc aggcccggtc agcggcgcat cggctgacgg ttgaaagcga gcgactgcag
     4081 gtggaaaaac gggcccagtc cacttcggcg cgggttgagc aactggccag gtcgcgcctg
     4141 cagatgcgtg gtgccaatcc ggccatcact gcctacgttg cctacacggc acaagttccg
     4201 gccgaaccgg tcgcaccgcg cgacccacgc aaacaggcgg ggtcaccatg agccgcagca
     4261 ttgcctacac ctccagtccg ctattggcca gcccgacacc ggtctggcgc agcaagttca
     4321 ttgtggccac catcgcgctg gcgtttgtcg gtctggccgg tcgcgccgcc tacatccagg
     4381 tgttcggcaa cgcttttttc cagcgcc
//
LOCUS       DAOSLL010000411         4398 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17561_length_4398_cov_5.065162, whole genome shotgun sequence.
ACCESSION   DAOSLL010000411 DAOSLL010000000
VERSION     DAOSLL010000411.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17561_length_4398_cov_5.065162"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1852)
                     /gene="pilQ"
                     /locus_tag="PLB25_15840"
     CDS             complement(<1..1852)
                     /gene="pilQ"
                     /locus_tag="PLB25_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HOE43081.1"
                     /translation="MKHNYRWIEWACWRLWLYAGVLLTSAVAQAQVAIQSVSGSLQSG
                     VEVVKIDLSQALTQVPSGFAIQSPARIALDFPNVVSAMGRSNVEVNQGNLKSVSVVQA
                     GNRTRVVLNLKQSAAYKTQLQGNSLLVVLDRVPGATPAATAQVFAENQSRDTLPLRDL
                     DFKRGTEGAGRVVVALPNNQVGVDIRQQGQTLVVEFMKTTLPEGLRRRLDVTDFGTPV
                     KTVTTFQSGDRVRMVIEPQGQWTHSAYQSNEQFVVEVKPIKVDPTKLTQGPGYSGEKL
                     SLNFQNIDVRSLLQVIADFTSFNVITSDSVNGAVTLRLQDVPWDQALDIILQAKGLGM
                     RKTGNVLWIAPKDEIIAKEKLELESKATVLSLEPVRTQSFQLNYAKAAEIASQLTGAG
                     GGDKSAARTLSPRGSATADARTNQLFVTDVPSKLEDVQQLLKKLDVAVRQVMIEVRIV
                     EASDNFGKSLGVKFGSSDLRAQQGGDGGYQLSGDNRIAIGTSYSNVVNTTGASGLPVD
                     TSGSFVNLPAVQPFGGTAATFGVSIFNAVANRFLNLEISALEADSKGRLVSSPRVVTS
                     DGKKATIKQGEQIPYQTSDPIKGATTSFKDANLRLEVTPQITPEGTVIMDL"
     gene            complement(1852..2394)
                     /locus_tag="PLB25_15845"
     CDS             complement(1852..2394)
                     /locus_tag="PLB25_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HOE43082.1"
                     /translation="MKRLPWVLTTALVFVLSACGRAADEELREWMAFQVSQAHSKVTP
                     LAEPKQFKPETYSQVSELHPFSNQKLTVALKKDSPQASASGALLTPELARRREPLEEF
                     PLDSMVMVGRMERLGKPVALITIGKLLYQVRLGDHLGLDYGLVTNISATEVSLREIVQ
                     DAAGEWIERTASLQLQERSK"
     gene            complement(2397..3068)
                     /locus_tag="PLB25_15850"
     CDS             complement(2397..3068)
                     /locus_tag="PLB25_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOE43083.1"
                     /translation="MATFPKVNVDLKAWFEGASNQFRGLDSKDPSSWPTLPRYAVFVF
                     LAAVCVAIFWYFLLSDKQVELTTEQQKELTLRDEFKVKLAKAVSLEALKKQREQVQQY
                     VTQLEKQLPSKSEMDALLSDINQAGIGRSLQFELFRPGQVAAKEYYAELPIAVRVTGR
                     YHDIGAFAADIANLSRIVTINNMSIVPARDGMLALDATAKTFRYLDAEEVNAQRKAVA
                     PGAKK"
     gene            complement(3077..3745)
                     /locus_tag="PLB25_15855"
     CDS             complement(3077..3745)
                     /locus_tag="PLB25_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOE43084.1"
                     /translation="MILINLLPHRELARKRRKDVFNVSLGFAALVGVLISVLVLLWFQ
                     AEIAAQQDKNQILQSEIKKFDAQIKEIATLEAEIVALRARQQAVEDLQSDRNMPVHLL
                     TELVRQLPEGVYINSMRQDGQNVSLLGVAQSNERISELLRNLSGGSAWLARPELVEIV
                     TGSVSLAPKDQRRVANFNVRVKLVRSSEAEKAKAAAGTTGAAKLKGASATMPSVASSL
                     PLAK"
     gene            complement(3742..>4398)
                     /gene="pilM"
                     /locus_tag="PLB25_15860"
     CDS             complement(3742..>4398)
                     /gene="pilM"
                     /locus_tag="PLB25_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HOE43085.1"
                     /translation="DVEVLIAASRREKVQDIQGLAEAAGLKPVIIDVESYASRLATSR
                     LIANTPNQGIGNIVALFEVGAMTTSMQVIRNDEVLYDRDQAFGGAQLTQMIVRQYGFS
                     LEEAESKKRSDDLPEDYASVVLQPFIESLVQEIGRALQFFFTSTTHNKVDMVMLAGGC
                     AALPGLAAAVTKQTTFAASVANPFDGMELASGVKLNKMTREAPSYLTSCGLALRRFLQ
                     "
BASE COUNT          977 a         1270 c         1104 g         1047 t
ORIGIN      
        1 ccaagtccat gatgactgtg ccttccggcg taatttgcgg tgtcacttcc aatctcagat
       61 ttgcatcttt gaaactggtt gttgcgccct tgatcgggtc actggtctga taggggattt
      121 gttcaccctg tttgatggtt gcctttttac catcggaggt aacaactctg gggcttgaaa
      181 ccaatctacc cttgctatca gcctccaacg cagagatttc aaggttcaag aatcgattgg
      241 ccaccgcatt gaaaatggaa acaccaaagg tcgcggcggt tccgccaaag ggctgcacgg
      301 ctggcaagtt gacaaaactg cccgaggtgt caaccggaag accactggcc ccggtggtgt
      361 tgacgacatt gctgtaactc gtcccaatgg ctattctgtt gtccccggac aactggtatc
      421 cgccatcgcc accctgctgt gctctaagat cagatgaacc aaacttcact ccgagagatt
      481 tcccgaagtt gtcgctggcc tcgacaattc gaacttcgat catgacctgt ctgactgcca
      541 catcgagctt cttcagaagc tgttgaacat cttccagctt ggacggcacg tcggttacaa
      601 atagctgatt ggttcgggca tccgcggtag cgctgccacg agggctgagg gtgcgagcag
      661 cacttttatc cccgccgcca gcccccgtca actgcgatgc gatttcagcc gctttggcat
      721 agttcaattg aaacgactgg gtacggactg gttccaggct gagcacagtc gctttggatt
      781 caagttccag cttttccttg gcaatgattt catctttggg cgcgatccac agcacgttgc
      841 cggtcttgcg catacccaag cctttggcct gcaaaataat gtccagcgcc tggtcccaag
      901 gcacatcctg caagcgcaaa gtgaccgcac cgttgaccga atcagaagta atgacgttga
      961 aactggtgaa gtcggcaatc acctgaagga gcgaacgaac atcaatgttt tgaaagttca
     1021 acgacaactt ctcaccgctg tagccaggcc cctgcgtcaa tttggtgggg tctaccttga
     1081 ttggcttgac ctccaccaca aattgctcgt tgctttggta agcgctgtgg gtccactgcc
     1141 cctggggctc aatcaccatg cgcacccgat ctcccgactg aaaggtggtg accgttttga
     1201 ctggcgtacc aaagtctgtc acatcaagtc ttcttcgcag gccttcaggc aaggttgttt
     1261 tcatgaactc aacgaccagt gtctggccct gctggcggat atccacccct acctggttgt
     1321 tgggcaaagc aaccacgacg cgcccagcgc cttccgtgcc ccgcttgaag tcaagatcac
     1381 gcaggggcaa ggtgtcgcgg ctctgatttt cagcgaacac ctgcgcagtg gctgccgggg
     1441 tcgcgccagg aacacggtcc agaaccacca gcaacgagtt gccctgcagt tgggtcttat
     1501 aggcggctga ctgcttcagg ttcaatacca cacgcgtgcg gttccccgcc tgtaccacgc
     1561 tgaccgactt gaggttgcct tggttgacct ccacatttga tcgacccatg gcgctgacta
     1621 cattcggaaa atcgagtgca attctggccg gagactgaat ggcaaatcca gaaggcacct
     1681 gtgtcaaagc ctgactcagg tcaattttga ccacctcaac cccgctttgc aaagagccgc
     1741 tcacgctttg aatggccacc tgagcctgtg cgactgccga tgtcaacagg actcccgcat
     1801 atagccacag ccgccagcag gcccactcaa tccatcggta gttatgcttc atcattttga
     1861 cctctcttgc aactgcaagg atgcagtgcg ttcaatccac tctccggccg catcctggac
     1921 aatctcccgc aagctcactt cggtcgcgga gatattggtg accaacccgt aatccagccc
     1981 cagatggtca ccaagacgca cttggtacag cagtttgcca attgtgatca gcgccaccgg
     2041 tttacccaaa cgttccatgc gcccaaccat gaccatggag tcgagcggaa actcttctag
     2101 cggctccctt cggcgtgcca attctggtgt cagcagtgca ccgctggccg aagcctgcgg
     2161 agaatccttt ttcagcgcaa ctgtcaattt ctggttgcta aaaggatgca gttcagagac
     2221 ctggctgtag gtttcgggct tgaactgctt gggttctgcc agtggtgtca ctttagagtg
     2281 tgcctgactg acctggaaag ccatccactc gcgcaattct tcgtcagccg cgcgaccaca
     2341 agcgctaagc acgaacacca aagcggtggt caggacccat ggaagacgct tcatcattat
     2401 ttttttgccc caggtgcaac cgctttacgc tgtgcattga cttcttctgc gtcgagataa
     2461 cggaaggtct tggctgtggc atctagagcc agcatcccgt cccttgcggg cactatggac
     2521 atgttgttaa ttgtgacgat gcgagacaaa ttcgcaatat cggcagcgaa agcacctatg
     2581 tcgtggtaac gacctgtcac acggaccgca ataggcaact ctgcatagta ctctttggcg
     2641 gcgacctgtc caggcctgaa aagttcgaat tgcaggctcc gcccgatgcc agcctggttg
     2701 atgtcagata acagggcatc catttcggat ttgcttggca attgcttttc aagctgagta
     2761 acgtactgtt gcacctgctc gcgctgcttc tttagcgctt caaggctgac ggccttggcc
     2821 agcttcactt tgaactcgtc gcgcaaagtg agttctttct gctgttcagt ggtcagttcc
     2881 acctgtttgt cgctgagcag gaaataccag aaaattgcca cgcagactgc cgcaagaaaa
     2941 acaaaaacgg catacctcgg cagtgtcggc caacttgacg gatccttgct gtccaggcca
     3001 cgaaactggt tcgaagcacc ttcaaaccag gctttcaagt ctacgttgac cttggggaat
     3061 gttgccatgg aatgccttac ttggccagcg gtagagagga tgccacagag ggcatggtag
     3121 ctgaagcgcc cttcagcttg gctgcaccag ttgtgcccgc tgcagccttg gctttttctg
     3181 cctcgctgga cctgaccagc ttgacgcgga cattgaaatt tgcaacacgc cgctgatcct
     3241 tgggtgccaa gctgacgctt ccggtgacga tctcaaccaa ttctgggcgg gctagccatg
     3301 ccgaacctcc gctgaggtta cgcaacaact ccgatatgcg ctcattcgat tgtgcaactc
     3361 caagcaagga cacattttgt ccgtcttggc gcatgctgtt gatatataca ccttcaggca
     3421 gttgccgcac caattcggtc aacaaatgca ccggcatatt gcggtctgac tgcaagtcct
     3481 ccacggcttg ctgacgcgcc ctcaatgcca caatctccgc ctccagggtc gcaatttcct
     3541 tgatctgagc atcaaacttc ttgatctcgc tctgtagtat ttggtttttg tcctgctgcg
     3601 cggcgatttc ggcctgaaac cagagaagta ccagaactga aatcagtaca ccaacaagtg
     3661 cggcaaaccc aaggctgacg ttaaatacgt ctttacggcg cttgcgcgca agttcgcggt
     3721 gcggcagcag gttgatcaga atcactgcag aaacctccgc aatgccaagc cgcaggaggt
     3781 gaggtaagac ggtgcctcac gggtcatttt gttgagtttg acaccactcg ccaactccat
     3841 tccgtcgaac ggattcgcca cgctagcggc aaaagtagtt tgcttggtga ctgctgctgc
     3901 caaccccggc aacgccgcac atccacctgc cagcatcacc atatcgacct tgttgtgcgt
     3961 ggtgctggtg aaaaaaaact gtagggcgcg cccgatttcc tgaaccaaac tttcgataaa
     4021 cggctgcaaa accacagagg catagtcctc tggcaggtcg tcactgcgtt ttttgctctc
     4081 tgcctcctcc agggaaaaac catactggcg aacaatcatc tgggttaact gcgccccgcc
     4141 aaaggcttga tcgcggtcat acaacacttc atcattgcga ataacctgca tgctggtggt
     4201 catggcacca acttcaaata aagccacgat gttgccaata ccctgattcg gcgtattcgc
     4261 aatcaagcgg ctggtggcca gtcgggaggc atacgactcc acatcaatga tcactggctt
     4321 gagccccgca gcttcggcca gtccttgaat atcctgcact ttttcacgac gtgaagctgc
     4381 gatcaggact tctacatc
//
LOCUS       DAOSLL010000412         4381 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17649_length_4381_cov_5.022423, whole genome shotgun sequence.
ACCESSION   DAOSLL010000412 DAOSLL010000000
VERSION     DAOSLL010000412.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17649_length_4381_cov_5.022423"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /gene="rho"
                     /locus_tag="PLB25_15865"
     CDS             <1..707
                     /gene="rho"
                     /locus_tag="PLB25_15865"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HOE43086.1"
                     /translation="MMQHIAHAIAANYPNSHMMVLLVDERPEEVTEMQRTVKGEVIAS
                     TFDEPAARHVHVAEMVIERAKRLVELKRDVVILLDSITRLARAYNNVVPSSGKVLTGG
                     VDSNALQRPKRFLGAARNVEEGGSLTIIATALVDTGSRMDEVIFEEFKGTGNSEIHLD
                     RRLYEKRVFPSIQLNRSGTRREELLLQPDILQKTRILRQFLYNMDEIESMEMVLKQMK
                     ATKTNSEFFDLMRRGG"
     gene            807..1064
                     /locus_tag="PLB25_15870"
     CDS             807..1064
                     /locus_tag="PLB25_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HOE43087.1"
                     /translation="MKKGIHPNYREICFVDLSNGFKFVTRSCASTKEKITLEDGRELP
                     LYKLDTSSESHPFYTGTQKSVDNMGGRVEKFRNRFGKPTAK"
     gene            1163..2872
                     /locus_tag="PLB25_15875"
     CDS             1163..2872
                     /locus_tag="PLB25_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43088.1"
                     /translation="MNQPTPAIVAQGAVKRLSRVALWLFCLAYVLPGFVGRGPWKRAD
                     INTFGYMAEMARGNTEWFSPVLGGLGPEVDALLPYWLGAWAISLSPAWLPIDMAARIP
                     FILLLIITLMATWYGTYYLARSPMAQPVAFAFGGEAEPTDYARALADGGLLAFIACLG
                     LAQLAHETTPALAQLCFTAVFFFAMAALPYRLMAPGLGAAAGLLGLSLSGAPTMALAL
                     GLGCTAIYFLAVGNNNEVRKHKQSRAIIVLAITLACALLATGWQLWRWRIELPHANWS
                     EWQRLGRLFLWFTWPTWPLACWTLWRWRHQLFGARFSSRHLWLPLWFVMVAAAATLTT
                     GAADRSLLLALPALAALAAFALPTLGRSLSALIDWFTLIFFSGCAVIIWVVWISMQTG
                     VPAKPAMNVARLAPGFVHDFSPALFLIALFASGIWAWLVIWRAGRHRSAIWKSVVLPA
                     GGAALCWLLLMTLWLPLLDFARSYEPLVRQAMAIMAGKPNCVSTLGLSRSQTAAFQFY
                     GELTLKPIASGSPCSWLIADGGLPGTTATDIIQSTPWQARGTIHHPADPQEKLALFER
                     RPD"
     gene            complement(2869..4197)
                     /gene="phoR"
                     /locus_tag="PLB25_15880"
     CDS             complement(2869..4197)
                     /gene="phoR"
                     /locus_tag="PLB25_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HOE43089.1"
                     /translation="MTWRILFFLFCLCVGGLSGWLLLERWNFGYGDRVGLVVAALFWL
                     ALDASGAGRLLGYLRASGTAEPARLWGIWGEVAMRVRRLVRDRDRQLTESHRRLQDFL
                     AAFQSSPNGVVLLDRDACIEWCNQTATQHFGILAQRDLAQAIANLVRDPAFVAYLASR
                     DYQRSITMPGRQHSAARPVLLSVQLHPYGEGRTLLLSRDITALEQAEAMRRDFVANVS
                     HEIRTPLTVLAGFVETLQTLQLDESERQHYLALMTQQSSRMQTLVSDLLTLSRLEGSP
                     APDLSRLTPFPQLIEQLRQDAAALSQTRTLELERPHLLVFENNFEGQIAGNVSELLSA
                     MGNLVSNAIRYTPAGARITVQSELLLDGSLVFSVQDTGPGIAPEHLGRLTERFYRVDR
                     SRSRETGGTGLGLAIVKHVAQRHGATLSIESSLGKGSTFRIMFPSDRIKP"
     gene            complement(4197..>4381)
                     /locus_tag="PLB25_15885"
     CDS             complement(4197..>4381)
                     /locus_tag="PLB25_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOE43090.1"
                     /translation="TYPERVHSRSQLLDKVWGDHVFIEERTVDVHVKRLREALGLEAG
                     LMVETVRGAGYRLTAS"
BASE COUNT          937 a         1266 c         1224 g          954 t
ORIGIN      
        1 tgatgatgca gcacattgcc cacgccattg ccgccaacta cccgaacagt cacatgatgg
       61 tgctgttggt tgatgagcgc cctgaggaag tcaccgaaat gcagcgcacc gtcaagggtg
      121 aggtcattgc atcaactttt gatgaaccag cagcgcggca cgtgcatgtg gctgaaatgg
      181 taattgaacg cgccaagcgc ctagttgagc tcaagcgtga cgtggtgatc ttgctggatt
      241 cgatcacccg tttggcgcgc gcctataaca atgtggtgcc atcgtcaggc aaggttttga
      301 ctggcggtgt ggattcaaat gcgctgcagc ggcccaagcg ttttttgggt gctgcccgca
      361 atgtggaaga aggcggctcg ttgaccatca tcgccactgc gctggtggac actggcagcc
      421 gcatggacga agtcattttt gaagaattca aaggtactgg caacagcgaa atccacctgg
      481 atcggcgact ctatgaaaag cgggtgtttc cctcgattca actcaaccgc agcggaaccc
      541 gccgcgaaga gctgttgtta caaccggata tcctgcaaaa aacccgcatc cttcggcaat
      601 tcctctacaa catggatgaa atcgagtcga tggaaatggt gctcaagcag atgaaagcca
      661 ccaagaccaa cagcgagttc tttgacctga tgaggcgagg cggctagtct ataatcacag
      721 gctttcagcg aaaagtacga gtagcttgaa attgcgcagt ggattgtgca agggctgacg
      781 cggctaacgc aactgatgga gtcaatatga aaaaaggcat tcaccccaac taccgcgaaa
      841 tttgtttcgt ggatctgtca aatggcttca agttcgtgac ccgttcttgc gccagcacaa
      901 aggaaaaaat caccttggaa gatggtcgcg agcttccgct gtacaaactc gatacatcca
      961 gcgaatcgca ccccttttat accggcacgc agaagtcggt agataacatg ggcggccggg
     1021 tcgagaaatt ccgtaaccgc tttggcaagc ccaccgccaa gtaagcggct ctagatgctg
     1081 tgccaaaagg cagccggtca taccgcgctg cctttttctt tgcaccggag cgtttttcac
     1141 tccgcctgaa cctgtactgc ccgtgaatca accgacacct gccatcgttg cacaaggtgc
     1201 tgtcaagcgc ctgtcccgag ttgcactgtg gctgttttgc ctagcctacg tgttgcccgg
     1261 gtttgtcggg cgcgggccgt ggaagcgcgc cgacatcaac acgtttggct acatggcgga
     1321 aatggcccgc ggcaatacag agtggttcag tccggtactt ggcggacttg gacccgaggt
     1381 tgacgcgctg ctgccttact ggctgggggc ctgggccatc agcctgtcgc cggcctggtt
     1441 acctatcgac atggcagcac gtattccttt catactgctg ctgatcatca cgctgatggc
     1501 cacctggtac ggcacctatt acttggcgcg aagtccgatg gcacagccgg tggcctttgc
     1561 ctttggcggt gaagccgagc ccacagacta tgctcgtgcc ctggctgacg gtggactgct
     1621 ggccttcatt gcatgtcttg ggctggctca actcgcgcat gagaccaccc cagcactggc
     1681 gcagctctgc tttaccgcgg tgtttttttt cgcaatggcc gccctgcctt atcgattgat
     1741 ggcaccgggc ctgggtgctg ccgcgggact gttgggtcta tccctgtctg gcgcaccgac
     1801 catggcgctg gcacttggac tgggatgcac ggcgatctat tttctggcgg tcggcaacaa
     1861 caacgaggtc agaaaacaca agcaatcaag ggcgatcatt gtgttggcaa taactttggc
     1921 ctgcgcgctg ttggcaaccg gatggcaact gtggcgctgg cggattgagt tgccgcatgc
     1981 caactggagc gagtggcagc gactgggacg cctttttttg tggttcacct ggcccacttg
     2041 gccgttggca tgctggacac tctggcgttg gcgtcaccag ttgtttggag ccaggtttag
     2101 cagccgacat ttgtggttgc ccttgtggtt tgtgatggtg gccgccgcag ctacgcttac
     2161 caccggtgcg gctgatcgat cgctgctatt ggccttgccc gcactggcgg cacttgcagc
     2221 atttgcgcta ccaacgctgg ggcgtagcct gagcgcgttg attgactggt tcacccttat
     2281 ctttttcagc ggctgtgccg tgatcatttg ggtggtgtgg atttcaatgc aaactggcgt
     2341 tccggccaag cccgccatga atgttgcgcg actcgcaccc ggttttgttc atgacttttc
     2401 acctgcttta tttttgatag cactcttcgc aagtggaata tgggcttggc tggtaatatg
     2461 gcgagcaggg cgacaccgct cggcaatctg gaaaagcgtt gttctgccag cgggtggagc
     2521 cgcgttatgt tggctactgt tgatgacatt gtggttaccc ctattggact tcgcgcgcag
     2581 ttacgagccg ctggtgcgac aggccatggc gatcatggca ggtaagccaa actgcgtttc
     2641 aaccttgggt ctgagccgaa gtcagactgc tgcgtttcag ttttatgggg aactgaccct
     2701 caagcccatt gcatcgggca gcccctgctc gtggctgatt gcagacggcg gtttgcctgg
     2761 cacaactgct accgacatca ttcaaagtac cccatggcaa gcacgtggca ccattcacca
     2821 cccggctgat ccccaggaaa aactggctct tttcgagcgt cgaccggatt agggctttat
     2881 tcggtcagaa ggaaacatga tccgaaatgt ggatcctttt cccaggctgc tttcaatgga
     2941 cagcgtggct ccgtgccgtt gagccacatg tttgacaatg gccaatccca agccagtgcc
     3001 acctgtctcg cgcgaacgac tgcggtccac gcggtaaaag cgctctgtca aacgccccaa
     3061 atgttctggc gcgatgcccg ggccggtatc ctgcactgaa aacaccaggc tgccatccag
     3121 cagcagctcg ctttgcactg tgatccgcgc gccagccggt gtatagcgga tcgcattgct
     3181 aaccaaattg cccatcgcgc tcagcagttc gctcacattg ccggcaatct gcccctcaaa
     3241 gttgttttca aaaactagca ggtgcggccg ctctagttca agtgtgcgcg tctgtgacag
     3301 cgcagccgca tcttgcctga gctgttcgat cagttgtggg aaaggtgtca agcgtgacaa
     3361 atccggtgcc gggctgcctt ccagtctcga cagggtcagc aaatcgctca ccagtgtttg
     3421 catccggctc gattgctgcg tcatcaacgc caggtagtgt tgacgctccg actcgtcaag
     3481 ttgcagggtt tgcagtgtct cgacgaaacc agccagtacc gttaacgggg tccggatttc
     3541 atgcgacaca ttggcgacaa agtcgcgtcg catcgcctcg gcctgctcca gggcagtgat
     3601 atcccgggac agcaacaaag tccggccctc gccgtaaggg tgcaattgca cggacaacaa
     3661 caccggacgc gcggctgagt gctgacgccc gggcatggta atgctgcgct gatagtcgcg
     3721 actggccaaa taggcgacaa acgccgggtc gcgcacaagg ttcgcaatag cctgggccaa
     3781 gtcacgctga gccaagatgc cgaaatgctg ggtcgcggtt tggttgcacc actcaatgca
     3841 agcatccctg tcaagtaaca ccacaccatt cggcgaagac tgaaacgcag ccaaaaaatc
     3901 ctgcaatctg cgatgactct cggtcagttg acgatctcgg tcgcgcacca gtcggcgcac
     3961 gcgcattgcc acctcgcccc aaatacccca aaggcgcgca ggctcagcag tcccgctggc
     4021 acgcaggtaa cccaacagcc gccctgctcc gctggcatcc aacgcaagcc aaaacagcgc
     4081 tgccaccaca aggccgacgc ggtcaccgta gccaaaattc caccgctcaa gcagtagcca
     4141 acccgacaag ccaccgacac aaaggcaaaa cagaaagaac aggattcgcc aggtcatcat
     4201 gaagccgtta accgataccc cgcaccacgc acggtttcaa ccatcaaccc ggcctcaagc
     4261 cccaaggcct cgcgcagacg cttgacgtgg acatccactg tccgctcttc gatgaaaaca
     4321 tggtcacccc agaccttgtc aagcaactgg gatcggctgt gaacacgctc cggataggtc
     4381 a
//
LOCUS       DAOSLL010000413         4371 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17719_length_4371_cov_4.613763, whole genome shotgun sequence.
ACCESSION   DAOSLL010000413 DAOSLL010000000
VERSION     DAOSLL010000413.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4371)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4371
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17719_length_4371_cov_4.613763"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..686
                     /locus_tag="PLB25_15890"
     CDS             <1..686
                     /locus_tag="PLB25_15890"
                     /inference="COORDINATES: protein motif:HMM:NF025388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4238 domain-containing protein"
                     /protein_id="HOE43091.1"
                     /translation="AIRRVIDSEAFPNDSDWNLILNLLGLIAVRNPKMRGSFNDAREQ
                     VLRRLADLLASDEKVWNRNVAKARDAGESISEAVSFEQVKQFVEEGKYKIEFHPEGNL
                     RIEFNAFDDLLPLLGQRTWSVLVAPEGGPEFICSDHPVTVTWKQGHAGPVGFGLKETE
                     VFMPLGRRVGFYGVYETPLKQVVMCKPGHVATMNRRTAWNAERHVYSHHQNFFVWHDG
                     DIREVGCEV"
     gene            1167..2066
                     /gene="ltrA"
                     /locus_tag="PLB25_15895"
     CDS             1167..2066
                     /gene="ltrA"
                     /locus_tag="PLB25_15895"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HOE43092.1"
                     /translation="MKRDGTASDKHNAANAAVPPTQSDRPASVDEPIAGEHQSTPSLE
                     ISTLMTEVLHKENLKRALERVKRNKGAPGIDGMTVDELGAHLKANWPSIRAQLVAGAY
                     QPKPVKRVEIPKPGGRKRLLGIPTVVDRFIQQAVAQVVQQHWEPHFHPNSYGFRPGRN
                     AHQAVRYAQATIRQGFGWVVDIDLEAFFDRVNHDRLMVRVRQHVDAPDVLKLINRFLK
                     GGVQVDGQTQATIQGVPQGGPLSPVLANVVRDELDWVLDARGHRLARYADDCNIFVKS
                     QCAGERVMHNVTRLSSAIHCGSQ"
     gene            2063..2347
                     /locus_tag="PLB25_15900"
     CDS             2063..2347
                     /locus_tag="PLB25_15900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43093.1"
                     /translation="MNAQKSAVDRPRNRKFLGFTVSRNGARIKVADKAIDKLRNTIRE
                     LTRRTRGHRLIDIVQELKTALTGWKYVHTTQRASTLASTSFARALVSLSA"
     gene            3017..3574
                     /gene="phaP"
                     /locus_tag="PLB25_15905"
     CDS             3017..3574
                     /gene="phaP"
                     /locus_tag="PLB25_15905"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Members of this family are phasins (small proteins
                     associated with inclusions such as PHA granules). Note
                     that several different families of phasins have been named
                     PhaP despite very little sequence similarity to each
                     other; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01841 family phasin"
                     /protein_id="HOE43094.1"
                     /translation="MNKTAEKLVATAKASVESAQALATKTQAAAEKLVELNMLTSMAV
                     LSDSFEYAKSVLGAKDPQALVALQAALAKQAAEKAATYAQEIQKIVSAVNAEYAKASQ
                     ANMADVQRGISNLVESTRSNVPAGSEPAMAFFNNAVNASQSSFVSAQNSAKQAVEAAQ
                     ANFSAFTAQAVETVKNLGEATKGAN"
BASE COUNT         1072 a         1209 c         1207 g          883 t
ORIGIN      
        1 ccgcaattcg cagagtaatc gacagcgagg cattcccaaa cgatagcgac tggaacctga
       61 tcttaaacct gctgggcctt attgcagtaa gaaatccaaa gatgcgtggg tcctttaacg
      121 acgcaaggga gcaagtgctc aggaggcttg ccgatctatt agcgtctgac gaaaaagtct
      181 ggaatcgcaa tgtagcaaag gcacgggacg caggcgagag catcagcgaa gcggtctcct
      241 ttgaacaggt caagcagttc gttgaagagg gaaaatacaa aattgagttt catccagaag
      301 gcaacttgcg tatcgagttc aacgcctttg atgatcttct accgctcttg gggcaaagga
      361 catggtctgt cttggtggcg cccgaaggag gaccagagtt catttgctca gaccatcctg
      421 ttaccgtcac ttggaaacaa ggtcatgctg ggccggtagg ttttggcctt aaggaaaccg
      481 aagttttcat gccgcttggg cggcgcgtag gcttctatgg ggtctacgaa acgccgctga
      541 agcaagtggt tatgtgcaag cccggccacg tagcgacgat gaacaggcgt actgcctgga
      601 atgcagagcg tcatgtctat tcacaccatc aaaatttctt cgtttggcat gacggcgata
      661 ttcgggaggt ggggtgtgag gtctaactgc tcgttccatc ggacggcctt cggccgccgc
      721 tgaactctac gtgtgtgccg tgcatggcac gaatccagcc gggtgaaagt cccggtgcca
      781 ggcttgtgga gccgaaggct aggccattgg gcaagtgcgt cgtcgcgagg cggggtgcag
      841 aggaagccca aggcgaaatc gcagggcgat gaacaaaaac cggatatgag gcgtgatgca
      901 ttcgggacga gcgggcacgt gaccgcgaag tcctctatct acaagtgggt ggcaacaccg
      961 acaagggtgc attttgtaaa tccggcgtct atgcgaggaa ggatttgtgt cttaccacgg
     1021 gagatctgcc atgtgtcgtg accgcgcaag cggcaaacga ctgagaaccg gggaaccggt
     1081 ttcgagcgca tggcagaagt cagcaaaagg catagtaggt gcggcccgca ccgaaggcac
     1141 cgagccttga acgatgagga attggtgtga agagagatgg gacagcatct gacaagcaca
     1201 acgcggccaa tgctgcggtg ccaccgacgc aatccgacag gcccgcgtca gtggatgaac
     1261 ccatcgctgg agaacaccag tccaccccat cattggaaat ctcgaccctg atgaccgagg
     1321 ttctgcacaa agagaacctc aaacgggcct tggaacgggt caagcgcaac aaaggcgcgc
     1381 caggcatcga cggcatgact gtcgatgaac ttggtgccca cctcaaagcg aactggccca
     1441 gcatacgcgc gcaactggtg gcaggcgctt atcagcccaa gccagtcaag cgcgtggaaa
     1501 tcccaaagcc aggcggaaga aaacgcctgc tcggcatccc caccgtggtg gaccggttca
     1561 tccagcaagc tgtagcccaa gtggtgcagc aacactggga gccgcacttc caccccaaca
     1621 gctacggctt taggccagga cgcaatgctc atcaagcagt gcgctacgcc caagccacca
     1681 tccgacaagg attcggctgg gtggtggaca ttgatctgga ggcgttcttt gaccgcgtca
     1741 accacgaccg cctgatggtg cgcgtgcgcc agcacgtgga tgcacccgat gtactcaaac
     1801 tcatcaaccg ctttctcaaa ggcggtgtgc aggtggatgg ccaaacccaa gccacgatac
     1861 aaggggtgcc gcaaggcggc cctttgtcgc cggtgttggc caacgtggtg cgggacgaac
     1921 tggactgggt gctggacgcc cgaggccacc gccttgcccg ctatgccgat gactgcaata
     1981 tctttgtcaa aagccaatgt gcgggggagc gggtcatgca taacgtaacg cgcttgtcta
     2041 gcgcgattca ctgcggctca cagtgaacgc ccaaaagagc gccgtagaca gaccccgaaa
     2101 ccgcaagttt ctgggcttta ccgtcagccg aaatggtgcc aggatcaaag tggccgacaa
     2161 ggccatcgac aaactcagaa acaccatccg cgagctgacc cgtcgcacac gtggtcaccg
     2221 tttgatagac atcgtgcaag agttaaagac cgccctgacc gggtggaaat acgtgcacac
     2281 gacccagcgt gcatcgacat tggcgtcgac ttcatttgcg agagcgctag tttccctttc
     2341 ggcatgaccc tcaagtcaca ggtagcgcga gcgctcaaga gcgcgccgct cacgccgacg
     2401 cagctctcaa ccatccgcac tcgggttgca agcatgttgc tggatgggta gggactccac
     2461 agcggtcctc cacttcccaa gccagaccta gtaaaggttt gaagtcaggg aaggatttgc
     2521 acagcgccgc cagatgctgc cgcgcctgca ccccatcacc aatgtctacc aatcggcgca
     2581 ctgctacgat ctggtcagca ttgggtgacg gggggagttt cttgctttgg ctgattcgtg
     2641 acatctgtgg ttgaattggc ttgggctgga ggtcgctgaa cattatcctg ccagacgaca
     2701 catagcttgt ctgggacggc agctcatcgc agccgattct tatccccaaa acccgttgtc
     2761 aacactgtga acaactagcc gaacaattct ccaaccccgc gccagccatg acgttccgag
     2821 gtgtgctaaa acaatgagca acaccgacca tgaagcgccg attaccgctt cttggcgaag
     2881 tttctttgtc gctgctgtac tacaacaaaa acaccctttc cactcgcatt ctccctacaa
     2941 tgcattttgc tgcactgcac cataaacccc acaaggtttg cgtttcagtt gtcatgaacc
     3001 acccgaggaa aatgaaatga acaaaaccgc tgaaaagctc gtcgccactg ccaaagcaag
     3061 cgttgaatca gcccaagctc tggcaactaa aactcaagct gccgccgaaa aactggtcga
     3121 actcaatatg ctgacctcga tggcggtatt gagtgattcg ttcgagtatg caaagtccgt
     3181 cctgggggcc aaggatcctc aggcattggt cgcgctacaa gccgctctgg ccaagcaggc
     3241 agccgagaag gctgccacct acgctcagga aatccagaaa atcgtctcgg ctgtcaatgc
     3301 agagtacgcc aaggcttcac aagcaaatat ggcagatgtc caaaggggca tctcaaatct
     3361 ggtcgaaagc actagatcaa acgtcccggc aggttctgag cccgctatgg cgtttttcaa
     3421 taacgccgta aatgccagtc agtcctcatt cgtgagcgct cagaattccg ccaagcaagc
     3481 cgtcgaagct gcccaagcca acttctctgc atttacagct caagcagtcg aaaccgtcaa
     3541 gaacttgggc gaggcgacca agggagccaa ctgattttgc aaaagtaagc gggacgactc
     3601 tttttgatgc cgctcatgct ttgagcttca gcatttcccg ctgctgcacc aaccccagcc
     3661 gccaccgtat cttgcttggc atgcagagca aaacaaaaaa ttcaccactg cgcaatgacg
     3721 gggttgcctt gattgatgca cgagacctgc tgctcgaaga agtccatttc aaatggctta
     3781 tggctggact gggttgctgg attgacatgg cacgctttcg cagcgattca tcgtatgcca
     3841 cccggtacct gaaattggca gccagtgctc aatgccagtc gctgcgaaaa tgtgcgactg
     3901 tattgcttgc aaagagcggt ttgtgagtga ctcatttgta aagccaagat cggtgcaccg
     3961 tttgaccgga cttcacctgc ggctaccact ttttgaagcg cttgttttct cgcatacgag
     4021 atagcagcac cgcacccagg tcgccatttc aacagtttag cgcggcaaag ctaaagtccc
     4081 gattgttgac gacgcatcct accagtggta gccaaactcc atcatggtgt ggcatgactg
     4141 ggccatggcg ttaatccggc ccttatgcgc agtagtgagc agcggtaccg ggccagaccc
     4201 cagaagtcgg taccctttgg acaggggaat tgctcgcttg agcatgccga catcgtcttg
     4261 tcactgcact atggaattga ttcctgcgaa gtttggcagc aagcgcagcc actggcttga
     4321 taaaggtgaa aatgcccaat cactggaaca tcatttgcac ttcagaccgg a
//
LOCUS       DAOSLL010000414         4361 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17762_length_4361_cov_5.112866, whole genome shotgun sequence.
ACCESSION   DAOSLL010000414 DAOSLL010000000
VERSION     DAOSLL010000414.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17762_length_4361_cov_5.112866"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(397..864)
                     /locus_tag="PLB25_15910"
     CDS             complement(397..864)
                     /locus_tag="PLB25_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010395969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HOE43095.1"
                     /translation="MNKLISLLVLCTASAMASGETIGDVSTTFKLLSPNDKVVVDVFD
                     DPAVDGVACYLSHAKTGGYKGAMGLAEDTADASVACRQVGPIGFKGKIDKQDEVFNAR
                     SSFLFKHVRVVRMVDVKRNTLVYLVYSDKIIDGSPKNSVTAVPVPAAQPIPVK"
     gene            complement(875..1813)
                     /locus_tag="PLB25_15915"
     CDS             complement(875..1813)
                     /locus_tag="PLB25_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HOE43096.1"
                     /translation="MQTPIHLLNGVAALFKVGLIRVQGDDAAKFLHGQLTNDFVALGP
                     GEARLAGFCTSKGRLQASFIGFKLSPTDILLVCDLALLPATLKRLSMFVMRAKAKLSD
                     ASADFVLFGLAGQALQNAIELRAISAFPTWARAYFDGVSLVSLHPADGVARALWVAPS
                     GSPAPAGAVLSQALWDWSEVRSGIATITPSVAEAFVPQMLNYESVGGVNFKKGCYPGQ
                     EVVARSQFRGTLKRRAYLAHCASPMKVGEEVFSSDDQGQPVGTVAQTAAAPASGFDAI
                     ISMQISAYESGGVRLGAVDGPPLMLSPPPYPLLADI"
     gene            1861..2844
                     /gene="mltG"
                     /locus_tag="PLB25_15920"
     CDS             1861..2844
                     /gene="mltG"
                     /locus_tag="PLB25_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HOE43097.1"
                     /translation="MRRLIGLLLVLVLLLAGASWWWVNHRLTLIGGVVELAIEPGTTP
                     RAVAQAAVEAGVETSPLALYWWFRLSGQARLIKAGSYEFEGELTPASLLSKLVRGDET
                     LRAVTLVEGWTFRQVRAALRKEEHLAQDTKGLEPDLIMQKLDRAGLPAEGRFYPDTYN
                     YAKGSSDLALLKRALRAMDAHLSAAWQARQSDTPLKTPDELLTLASIIEKETGRASDR
                     PMISSVFSNRLRAGMPLQTDPTVIYGMGEQFDGNLRRRDLQADTPWNTYTRGGLPPTP
                     IAMPGKAALLAAAQPATSGALYFVARGDGSSQFSENLAAHNRAVNKYQRRQ"
     gene            2861..3508
                     /gene="tmk"
                     /locus_tag="PLB25_15925"
     CDS             2861..3508
                     /gene="tmk"
                     /locus_tag="PLB25_15925"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HOE43098.1"
                     /translation="MKHTGLFISFEGIDGAGKSSQIGALAQVFEAQGRVVTLTREPGG
                     TPLAEKIRALVLNDAMDAMTEALLVFAARRDHLQQLIAPALARGEVVLSDRFTDASFA
                     YQGSGRGFDLDMLSYLERMVQTAKGLEGFFLRQPDLTLWFELDPRVAVQRLAGVRLPD
                     KFESQPLDFFSRVSNGYLQRWQQQPQRFARIDADRLPESVRQSVLQAVQQKGWLT"
     gene            3505..>4361
                     /locus_tag="PLB25_15930"
     CDS             3505..>4361
                     /locus_tag="PLB25_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010105139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HOE43099.1"
                     /translation="MSAGSVVTASCVAPWIAAQAQSLLAQRGHAWLLAGPSGLGQFDL
                     ALALVRAWLCDQPSGQGACGHCGSCHAIDVHAHADLSVLMPETVMLDLGWPLSEKAQE
                     EIDAKKRKPSKEIRVESMRDTVEFAQRTSARGRGKAVLVFPAERMNLFTANALLKTLE
                     EPAGDVKFVLATDAAHQLLPTIRSRCLGHSMAWPDQLSAITWLQQQGLSEARAAVSLR
                     AAGGRAQDALLFSTLGDTWKLLPRAVQRGDVSVFKDWTPAQLVDVLHKICHDLLLVKA
                     GAVPRFFKPG"
BASE COUNT          886 a         1276 c         1328 g          871 t
ORIGIN      
        1 cccctaccgc tggcattgca agtcgggcaa ttgaagcgat gggccgtggt gtttcttcgc
       61 ggcaatggac tcgctggcag tcaccttggc gttgccgccc ggaccagcga tggcaccaat
      121 gagtgcaaat ggttgtaaca aatcagcctc tagcccccgt ctggcaagcg ctggcagcta
      181 ttgattttga tatttctggc acggtgtcct gatgccgctg cacacctcac cgggttgcct
      241 catcttggta tttgtgacaa atcagcttct agccttgcac acacaggggc ggggtgaagc
      301 tcgcgtgtga tgcctgaaaa gtgctgttta gctattaaaa aagaagctat cagcgctttg
      361 ctggcaaggg ccagagccat atttcatttg caacgcctac ttgaccggta tcggctgggc
      421 agcgggcacc gggacggctg tcacgctgtt ttttgggctg ccgtcaatga ttttgtcgga
      481 gtacaccaaa tacaccagcg tgttgcgctt cacatcgacc atgcgtacca cccgcacgtg
      541 tttgaacaaa aacgatgagc gcgcgttgaa tacctcgtct tgcttgtcga tcttgccttt
      601 gaacccgatt gggccgactt ggcggcaggc aacagacgca tccgccgtgt cttcagcaag
      661 acccatcgca cccttgtaac cgccggtctt ggcgtgtgac aggtaacagg caacgccatc
      721 gaccgccggg tcgtcaaaaa catccaccac caccttgtcg ttgggactga gtagcttgaa
      781 ggtggtgctg acatcgccga tggtttcgcc gctggccatg gcgctggcag tgcacaggac
      841 aagcaaggag atcaatttgt tcatgacaac actttcagat atcggccagc aaggggtagg
      901 gcggcggcga cagcatcaac ggtggcccat cgactgcccc caggcgcaca ccaccagact
      961 catacgcgct gatttgcatc gatatgatcg catcaaaacc gctcgcgggc gcggcagcgg
     1021 tttgcgccac ggtgcccaca ggctggccct gatcgtcgct gctgaacact tcctcgccaa
     1081 ctttcatggg gctggcgcaa tgcgccagat aggcccggcg cttcaatgtg cctcgaaact
     1141 ggctgcgcgc aaccacctcc tggccgggat agcagccctt tttgaagttc accccgccga
     1201 ccgactcgta gttgagcatt tgcggcacaa aggcttcagc aaccgatggg gtaatcgtcg
     1261 caatgccact gcgcacttcg ctccagtccc aaagggcctg cgacagcacg gctccggccg
     1321 gggccgggct acccgaaggt gccacccaga gcgcgcgagc caccccatcg gccggatgga
     1381 gactgaccaa agatacacca tcaaaatagg ctctagccca agtgggaaaa gcgctgatag
     1441 ctcttagttc aatagcattc tggagagcct ggccagccag cccgaataac acaaaatcgg
     1501 cgcttgcatc gcttagtttg gccttggcac gcatcacaaa catcgaaaga cgtttgagcg
     1561 tggcgggcaa aagggccagg tcgcagacca gcaggatgtc agtcgggctc agtttgaagc
     1621 caataaagct cgcttgcagc cgacccttgc tggtgcaaaa tccggccagc cgggcctcgc
     1681 cggggcctaa cgccacgaag tcgttggtca actgaccgtg cagaaacttg gcggcatcgt
     1741 cgccctgcac acgaatcagg ccaaccttga acagcgccgc aacgccgttg agtaagtgaa
     1801 tgggtgtttg catggcgagg attatgatgc gctgctattt tcccggaagg ttacagggcc
     1861 gtgcgtcgtc tcattggttt actgctggtg ctggttttgc tgcttgcagg tgcatcctgg
     1921 tggtgggtta accatcgcct gaccctcatt ggcggggtgg tcgaactggc cattgagcca
     1981 ggcaccaccc cccgagctgt cgcgcaggcg gcggtcgaag cgggggtgga aacatctcca
     2041 ctggccttgt actggtggtt tcgcctgtcg ggccaggcca ggctgatcaa ggccggcagc
     2101 tatgaatttg aaggcgaact gactcctgcc tcactgctgt cgaagctggt gcgtggcgac
     2161 gaaaccctgc gtgccgtgac cctggtggag ggctggacct tcaggcaggt ccgcgcggcg
     2221 ctgcgtaaag aggagcatct tgcgcaagat acgaaagggc tggagcctga tttgatcatg
     2281 caaaagctcg accgtgcagg gttgccggca gaagggcggt tttacccgga cacctacaac
     2341 tacgccaagg gcagcagcga cctggcgctg ctcaaacgcg ccctgcgggc gatggatgcg
     2401 catctgagcg cagcatggca ggcccgccaa agcgacacac ctttgaaaac ccccgatgaa
     2461 ttgttgacgc tggccagcat tattgaaaag gaaaccgggc gtgccagtga ccgaccgatg
     2521 atctcctcag tgttcagcaa ccgcctgcgc gccggcatgc ccttgcaaac cgacccaacg
     2581 gtgatttatg gcatgggtga acagtttgac ggcaacctgc gccggcgcga cttgcaggct
     2641 gacacgccct ggaataccta cacccggggt ggcttgccac cgacacccat tgccatgccc
     2701 ggcaaagccg ccttgctggc ggcggcgcag cctgccacca gtggcgcgct gtattttgtc
     2761 gcgcgtggtg atggcagcag ccagttcagt gaaaatctgg cggcgcacaa ccgcgcagtt
     2821 aacaaatacc agcgcaggca atgagaattc aaccccatcc atgaaacata ccggactttt
     2881 catcagcttc gagggcattg acggggctgg caaatccagc caaattggcg cgctggcgca
     2941 agtctttgaa gcacagggca gggtggtgac gctcacccgt gagcccggtg gtaccccgct
     3001 ggccgaaaaa atcagggctt tggtgctgaa cgatgcaatg gatgcgatga ccgaagcgct
     3061 gctggtattt gccgcccggc gcgaccacct gcaacagttg atagcgccag cgctggcacg
     3121 cggtgaagtg gtgctgtctg atcggtttac cgatgccagc tttgcctacc agggcagcgg
     3181 ccgcggattt gacctcgata tgctctcata tttagagcgt atggtgcaga ctgctaaagg
     3241 gcttgaaggc ttttttctgc gccaaccgga cctgactctg tggtttgaac ttgacccccg
     3301 agtcgcggtc cagcggctgg caggggtgcg attgcccgat aaatttgagt cgcaaccgct
     3361 ggactttttt agccgtgtca gcaacggcta tttgcagcgc tggcagcagc agccgcaacg
     3421 ttttgcgcgc attgatgcgg accggctacc cgagtcggtg cggcaaagcg tgctgcaagc
     3481 ggtgcaacaa aagggttggc taacatgagt gccgggagcg tggtcacagc ttcttgcgtc
     3541 gcgccctgga ttgccgccca ggcccaatcc ctgctggcgc aacgtggtca tgcctggttg
     3601 ctggcaggtc cgtccgggct tgggcaattc gatctggcgc tggcgctggt gcgggcctgg
     3661 ctgtgcgacc aaccaagcgg gcagggcgct tgcggccact gcggcagttg ccacgccatt
     3721 gacgtgcacg cgcacgccga tctgtcggtg ctgatgcccg agactgtgat gcttgacctt
     3781 ggctggccac tgagcgaaaa agcacaggaa gaaattgacg ccaaaaagcg caaacccagc
     3841 aaggaaattc gggttgagtc gatgcgcgac acagtcgaat tcgcgcaacg caccagcgct
     3901 cgtggccggg gcaaggcggt gctggtgttt cccgccgagc gcatgaatct gttcaccgcc
     3961 aatgcgctgc tgaaaaccct ggaagagcct gcgggcgacg tgaagtttgt gctcgccacc
     4021 gatgccgcac accaattgtt gccaaccatt cgcagccgtt gcctgggtca cagcatggcc
     4081 tggcctgacc aactcagtgc catcacttgg ctgcagcagc agggcttgtc tgaagccagg
     4141 gcggcggtga gcttgcgtgc cgccggtggg cgcgcccagg atgcgctgct gttttcaacc
     4201 ttgggcgaca cctggaaact gcttccgcgc gcggtgcagc gcggcgatgt cagcgtgttc
     4261 aaggactgga ccccggcgca actggttgat gtgctgcata agatatgtca cgacctgctg
     4321 ctggtcaagg cgggtgccgt gccgcgattt tttaaaccag g
//
LOCUS       DAOSLL010000415         4349 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17831_length_4349_cov_4.460410, whole genome shotgun sequence.
ACCESSION   DAOSLL010000415 DAOSLL010000000
VERSION     DAOSLL010000415.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17831_length_4349_cov_4.460410"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1471)
                     /gene="ftsH"
                     /locus_tag="PLB25_15935"
     CDS             complement(<1..1471)
                     /gene="ftsH"
                     /locus_tag="PLB25_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HOE43100.1"
                     /translation="MNNPWFSKIAVWLVIAMVLFTVFKQFDTRPNTGAGYLGYSDFLD
                     EVKNNRIKSAIIQEGQSGTEIVAVTTDDRKVRTTATYLDRGLVGDLIANDVKFDVRPR
                     EEGSLLMTLLVSWGPMLLLIGVWIYFMRQMQGGGKGGAFSFGKSKARLLDENTNTVMF
                     SDVAGCDEAKEEVKEVVDFLKDPAKFQKLGGRIPRGLLLVGPPGTGKTLLAKSIAGEA
                     KVPFFSISGSDFVEMFVGVGASRVRDMFDNAKKNAPCIIFIDEIDAVGRQRGAGLGGG
                     NDEREQTLNQMLVEMDGFETNVGVIVVAATNRPDILDAALLRPGRFDRQVYVTLPDIR
                     GREQILNVHMRKVPLSQDVNPAVIARGTPGMSGADLANLCNEAALMAARRNARTVEMQ
                     DFEKAKDKILMGPERKSMVMPESERRNTAYHESGHALIGKLLPKCDPVHKVTIIPRGR
                     ALGVTMSLPAQDRYSYDKDYMLNQISMLFGGRIAEEVFMH"
     gene            complement(1595..2263)
                     /locus_tag="PLB25_15940"
     CDS             complement(1595..2263)
                     /locus_tag="PLB25_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HOE43101.1"
                     /translation="MKSAVKSSKVNRAWLNDHVNDTYVKLAKKDGYRARAAYKLKEID
                     ETLHLIKPGQTVVDLGSTPGAWSQYVRRKLSPKSATGGGAASGALNGRILALDILPME
                     PIEGVVFIQGDLREETVLAQLAAQLQGQPADLVLSDMAPNLSGIESVDAARIEHLVDL
                     ALAFAQAHLSAQGALVTKVFHGSSYDSLFKRLRETFVTVKRIKPKASRDRSSETFLVG
                     LVLK"
     gene            2312..2788
                     /locus_tag="PLB25_15945"
     CDS             2312..2788
                     /locus_tag="PLB25_15945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HOE43102.1"
                     /translation="MPQIQLSPAQRKVHRAEAHHLDPVVMIGGDGLSDAVKKEADAAL
                     NAHGLIKIRVFSDDRAAREAMFAALTDELNAAPIQHIGKLLVLWRPAPEREKTVNDER
                     LPGPRDVKVLKFSKRGGQRPEVKTLRVLGNQRLTTGGQIKRAKRKPVSIKKRNQQN"
     gene            complement(2972..3406)
                     /locus_tag="PLB25_15950"
     CDS             complement(2972..3406)
                     /locus_tag="PLB25_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HOE43103.1"
                     /translation="MKRVLSGAAIWIAAIWWGSLTTVGFYVVPMLFANLPSKALAGNM
                     AAKLFSAQTCVAMLCGLLLLLNDRLNQAAALVRQARVAIIFVSLGMLLALLSEFAVAP
                     RIVARDNLRLWHSVGTVLYMLQWGCAGLAFKELLAGRGHSAA"
     gene            complement(3422..3898)
                     /gene="greA"
                     /locus_tag="PLB25_15955"
     CDS             complement(3422..3898)
                     /gene="greA"
                     /locus_tag="PLB25_15955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HOE43104.1"
                     /translation="MATLPITKRGADILKAELHKLKTVERPAVINAISEARAQGDLSE
                     NAEYEAAKDKQGFVEGRIQEIEGKLSAAQIIDPTSLNADGRVVFGATVELEDEDSGDK
                     VTYQIVGEDEADIKLRLININSPIARALIGKEEGDVAEVQAPGGLRRYEVVAVSYK"
BASE COUNT          979 a         1341 c         1159 g          870 t
ORIGIN      
        1 ggtgcatgaa cacctcttcg gcgatgcggc ccccgaacag catgctgatc tggttgagca
       61 tgtagtcctt gtcgtagctg tagcggtctt gcgctggcag gctcatcgtg acacccaggg
      121 cgcgaccacg agggatgatg gtgaccttgt gcaccgggtc gcatttgggc agcagcttgc
      181 cgatcagggc gtggccagac tcgtggtaag cggtattgcg acgctcgctc tcaggcatga
      241 ccatgctctt gcgctcagga cccatcagga ttttgtcctt ggctttctca aaatcctgca
      301 tttccaccgt gcgggcattg cgccgggcag ccatcaaggc ggcttcgttg cacaagttgg
      361 ccagatcagc acccgacatg ccgggtgtgc cccgcgcaat cacggcgggg ttgacatcct
      421 ggctcaacgg caccttgcgc atgtgaacat tgagaatctg ctcgcggcca cgaatgtcgg
      481 gcagcgtcac atacacctga cggtcaaacc ggcccgggcg cagcaaggcg gcatccagaa
      541 tgtcggggcg gttggtggcg gccaccacaa tgacacccac attggtttca aaaccatcca
      601 tttcgaccag catctggttc aaagtctgct cacgctcgtc attgcccccg cccaaaccgg
      661 caccgcgctg gcgcccaacg gcatcgattt catcaatgaa aatgatgcat ggtgcgtttt
      721 tcttggcgtt gtcaaacatg tcacgcaccc gggaggcacc aacacccaca aacatttcga
      781 caaaatcgga gccggagatg ctgaaaaagg gtacttttgc ctcaccggcg attgacttcg
      841 ccagcaaggt cttgccggtt cccggagggc cgaccagcaa caaaccacgc gggatccgac
      901 cgccaagctt ctggaacttc gccgggtcct tcaagaagtc aaccacttcc ttgacttctt
      961 ccttggcctc gtcacagcca gccacatcgg aaaacatcac ggtattggtg ttttcgtcca
     1021 gcagacgtgc cttgctcttg ccaaagctga aggccccacc cttgccgccg ccctgcatct
     1081 gacgcatgaa ataaatccac accccgatca gcagcagcat cggaccccaa ctcaccagca
     1141 gcgtcatcaa aagcgagcct tcttcgcgcg ggcgaacgtc aaacttgaca tcgttggcaa
     1201 tcaaatcccc caccagacca cggtccaggt aggtggcggt ggttcgcact ttccggtcgt
     1261 cggtggtaac cgccacaatt tcagtgccgc tttggccttc ctgaataatg gcgctcttga
     1321 tgcggttgtt cttgacttca tcaagaaagt cagagtagcc cagatacccg gcaccagtgt
     1381 ttggacgcgt gtcaaactgc ttgaaaacag tgaataacac cattgcgatc accagccaga
     1441 cagcgatttt cgaaaaccat ggattgttca agcgaaactc caatagacac gcaaaaaaag
     1501 gggaacacca gatgcggccg gattttaggc gcatcactgt gccgggaaac tcttgcagat
     1561 gcaatcagcc acagcaggcg caaggaaatg tggcttattt caacactagg ccgaccaaaa
     1621 aggtctcgga cgacctgtcg cgcgaggctt tgggtttgat gcgcttgacc gtgacgaagg
     1681 tttcccgcaa gcgtttgaac aagctgtcgt agctgctacc gtgaaacacc ttggtcacca
     1741 gcgcgccttg cgcactgaga tgcgcctgcg caaatgccag cgccaggtcg accagatgct
     1801 cgatgcgcgc ggcatcgacc gactcgattc ccgacaggtt gggtgccatg tctgacagca
     1861 ccaaatcagc cggctgcccc tgcaattgcg ccgccagttg cgccagcaca gtttcttcac
     1921 gcaaatcacc ctggatgaat acaacgccct caatcggctc catcggcaaa atatccaatg
     1981 caaggatgcg accgttcagt gcgcctgaag ccgcaccgcc gccggtggcc gattttggtg
     2041 acagcttgcg ccgcacatac tggctccagg cccctggggt cgaacccaga tccaccaccg
     2101 tctgcccagg cttgatcagg tgcagggtct cgtcgatttc tttcaacttg taggcagcgc
     2161 gcgcccggta accgtctttt ttggccagtt tgacgtaggt gtcattgaca tggtcattga
     2221 gccatgcgcg attgaccttg ctgcttttga ctgctgattt cataaagtgt tgcccgttcc
     2281 gtaagcatgc aggctggccg ataatactgc catgcctcaa atacaactct cccccgccca
     2341 acgcaaggtg catcgcgccg aggctcatca ccttgaccca gtcgtcatga tcggcggcga
     2401 tggcctgagc gatgccgtca aaaaagaagc cgatgccgcc ctgaacgccc atggtctgat
     2461 caagatccgg gtgttttcgg atgatcgggc cgcgcgtgaa gccatgtttg ccgcgctaac
     2521 cgacgaactc aatgcagccc ccatccagca catcggcaaa ctgctggtac tgtggcgacc
     2581 ggcaccggag cgcgaaaaga cggttaatga cgaacgcctg cccggcccgc gcgatgtcaa
     2641 ggtgctgaaa ttcagcaaac gcggtggcca gcgccctgaa gtcaaaaccc tgcgcgtgct
     2701 cggcaaccaa cgcctgacca ccggtggcca gatcaagcgc gccaagcgca aaccggtcag
     2761 catcaagaag cgcaaccagc aaaactgatc accatgcctg aacaaacggc cagccggtgc
     2821 ccggcgaaag cacaggccct gtcgccagcg gctcattgca tttttgactc atggttgctc
     2881 aggcatcaag ccagcgccct ggtcacagac attgcaaacg caaggcaagg caagcgtcca
     2941 acgacatgga tgatttggca atggcatcag gtcaggcagc cgaatggccc cgcccagcca
     3001 gcagttcttt gaaggccaac ccggcacagc cccattgcag catgtaaagc acagtgccaa
     3061 cgctgtgcca cagccgcagg ttgtcccggg cgacgatgcg cggggcaacg gcaaactccg
     3121 acagcagtgc cagcaacatc ccaagtgata caaaaataat agctacccgc gcttgtctga
     3181 caagggctgc agcctgattt aacctgtcat ttagcaacaa cagcaagccg cacagcatgg
     3241 ccacacaggt ctgggctgaa aacaatttgg ccgccatgtt tccagccagc gctttggatg
     3301 gcaggttcgc aaacaacatc ggaaccacgt aaaacccgac cgtcgtcaaa ctgccccacc
     3361 aaatcgctgc aatccagatg gctgcgcccg acaagacccg cttcatggaa cccggcttcg
     3421 tttacttata gctgaccgcc accacctcat aacggcgcaa gccaccgggt gcctgcacct
     3481 cggccacgtc gccctcttcc ttgccgatca gcgcacgcgc aatcggactg ttgatattga
     3541 tcaggcgcag cttgatgtcg gcctcatcct caccaacgat ctggtaggtg accttatcgc
     3601 cagagtcctc atcctcgagc tccacggtgg caccgaacac cacgcgacca tctgcattga
     3661 gtgaggtcgg gtcaatgatc tgcgccgctg agagcttgcc ttcaatttcc tggatgcgcc
     3721 cctcgacaaa gccttgtttg tccttggccg cctcgtactc ggcgttttcg ctcaagtcac
     3781 cctgggcacg cgcctccgag atggcattga tgacagccgg gcgctcaacc gtcttgagtt
     3841 tgtgcagctc cgccttgagg atgtcagcgc cgcgcttggt aatgggaagg gttgccacgt
     3901 acagctccgt gaaaaaaggt gtcacaaaat gaaaccgccg aacgttgcgg tccggcggtt
     3961 gaccaggatt atgccgcgaa tcgcgacttc gttgctgttc tcgcacggat tcgatgcccc
     4021 gtgaaccagc aacgcacacg caacccatct atccgagcgg tcaggcagga agatgggtca
     4081 aacccgactt gttccatttc tcaatcatcc atgttctggc tgtctcgcca tgcctcgcgc
     4141 atgcaatgtc tgcggcaagg tgcccagaca cgaatgatgt agaaatggaa ttggccctga
     4201 ccttgacacc gcccaatgcc aaactcagta agaagtttcc tggcagaagg ctggcagaaa
     4261 aggttttgca agcggtgggt ggcatcgctc ttggcggctt aggatgtggt cgggaataac
     4321 tccccgtttt gcaccaagga gttgggatg
//
LOCUS       DAOSLL010000416         4340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17867_length_4340_cov_4.377130, whole genome shotgun sequence.
ACCESSION   DAOSLL010000416 DAOSLL010000000
VERSION     DAOSLL010000416.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4340
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17867_length_4340_cov_4.377130"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..950
                     /locus_tag="PLB25_15960"
     CDS             87..950
                     /locus_tag="PLB25_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE43105.1"
                     /translation="MFRPPSEAQSLILPLTNGCSWNKCTFCEMYSAPQKRFGARDEAE
                     IHNTIRLCGEQFSADVQRVFLGDGDAMVLSTRRLLAVLAAIKAHLPRVRRVSSYCLPR
                     NLRQKSVAELQQLREAGLSLVYVGAESGDDEVLKNVGKGESFETTREALDKLASAGIT
                     RSVMLLNGLGGQTLSSQHADNSARLINLTQPEFLATLVVSFPQGEDRFRQAWPLWQPL
                     DPHQLFVEMARLLGALELRRTVFRSDHASNWLVLKGVLGAEKDRLLQQVQDAINRPQA
                     ARLRPAWQRGL"
     gene            complement(1016..1315)
                     /locus_tag="PLB25_15965"
     CDS             complement(1016..1315)
                     /locus_tag="PLB25_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013597067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body protein"
                     /protein_id="HOE43106.1"
                     /translation="MASISSIALSGMNAAQVTLNSSAHNIANMNTAGFRRQEVAQTAQ
                     AGGGVSSALTTASAEGAAPETDVVTQLQAKNSFLANLAVFKTSDKMAGALLDKTA"
     gene            1500..2042
                     /locus_tag="PLB25_15970"
     CDS             1500..2042
                     /locus_tag="PLB25_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43107.1"
                     /translation="MTKLIFTLSVPNFLRPSRDRPPKNCLHARTGFFVDCQRLFILIG
                     CTPMKGGMAGSEQANDPAYPRTVFSRQTPITNVPTLNQWCNILLCYYMNHSMTISAKL
                     FMSGRSQAIRLPAKLRLAAKVVLIEQIGDGLWIRPEPEPTQNMGVWLQSFYDNTQALP
                     EDFLAERHDAPAQARDWVLD"
     assembly_gap    2184..2193
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2194..2475
                     /locus_tag="PLB25_15975"
     CDS             <2194..2475
                     /locus_tag="PLB25_15975"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapC toxin family PIN domain ribonuclease"
                     /protein_id="HOE43108.1"
                     /translation="AELRLGAAKLGAPRFTKAVETWLAGFDVQAWPLQATEHYAHIRA
                     KLEHDGNIFGAMDLLIASHALANDSVLVTNNAREFARVPGLAVEEWQLE"
     gene            2513..3598
                     /locus_tag="PLB25_15980"
     CDS             2513..3598
                     /locus_tag="PLB25_15980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020226879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HOE43109.1"
                     /translation="MQLVRDLTAAMSDNVGQAGDVGYTVINYNTAAQTLRCIESLAHC
                     TPPPAWVVVLDNASAEADFDKLKSGLRRADQCHIRLFRSDVNLGFAAGSNFLIDQLLD
                     IPACQYIGLLNNDAVAKPELVQLLRAALLETTHSIGMSGGRMHKLHHPEEVDTLGISL
                     YASLMPADRKAISDPYLGPTGGCCLLTRDFVQDVKATTGYCFDARFFCYCEDTDLALR
                     ANLLGYRPAYVDQLVALHEGQASAKTANNNFIAYHGLRNTMWMQWKCLPSSILLTHAP
                     WLLLAHLLTFTRQLFSGRSGLMLKVYRDALNLLPSVKTERVQLKASSRVPSASLAAHF
                     SRRFYRKGYICVVLSEWKARLSIAGRI"
     gene            3727..>4340
                     /locus_tag="PLB25_15985"
     CDS             3727..>4340
                     /locus_tag="PLB25_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE43110.1"
                     /translation="MSLGKSLWRNRQLIAQMTRRDVVGRYKGSAMGLAWSFFNPVFML
                     VVYTFVFSVIFKSRWGGAGGDDSKTQFAVVLFVGMIVMSLFSDVLNRAPSLILSNVNY
                     VKKVVFPLEILPVIATGAAVFHSMISLGVLLVAFVLFNGYLHWTLVFVPLVMLPLVIL
                     ALGFAWMLASLGVFLRDVGQFVGIVTTVMMFLSPVFYPVTAVPER"
BASE COUNT          895 a         1184 c         1224 g         1027 t
ORIGIN      
        1 aatcagaccg ccgccacaac agcagcgctc aaaagaggac aaacccggac gtgaagcagg
       61 ttttcccgat tcgttatgtt gagccggtgt ttcgtccgcc aagcgaggca cagtcgctga
      121 ttttgccgtt gaccaatggc tgttcgtgga acaaatgcac tttttgcgaa atgtacagtg
      181 cgccgcaaaa gcggtttggc gctcgcgatg aagcagagat ccataacacc atccgcctgt
      241 gcggcgaaca attctcagcc gacgtgcagc gggtcttcct gggtgatggc gacgcgatgg
      301 tgctgtcaac ccgacgactg ctcgcagtgc tggcggcgat caaggcgcat ttgcccaggg
      361 tgcggcgcgt gtccagctat tgcctgccgc gcaacctgcg ccagaaaagc gtggcggagc
      421 tgcagcaact gcgtgaggcg ggcttgtcgc tggtctatgt tggcgcagag tccggcgatg
      481 atgaggtctt gaaaaacgtc ggcaagggcg agagctttga gaccacccgg gaggcactcg
      541 acaaattggc ctcggccggt atcacccgct cggtgatgct gctcaatggg ctgggtggtc
      601 agacgctttc aagccagcat gccgacaact cggcgcgcct gatcaacctg acccaaccag
      661 agtttttggc cacattggtg gtcagtttcc cccagggcga agaccggttt cgccaggcct
      721 ggccgctctg gcagccgctt gacccgcacc aactgtttgt cgagatggcg cgattgctgg
      781 gtgcgctgga gctaaggcgc accgtgtttc gcagtgatca tgcgtcaaac tggctggttc
      841 tcaaaggtgt tctgggggcc gagaaagatc gcctgttgca acaggtgcaa gatgccatca
      901 atcgcccgca ggcggcgcga ttgcggccag cctggcaacg cgggctgtga tgccagtctt
      961 ttgtggtcag cggtaaccag gcaacagatg ctgcttgcgc agaccggttg gccagtcagg
     1021 cggttttgtc gagcaatgca ccagccatct tgtcgctggt cttgaacacc gcaagattgg
     1081 ccaaaaaaga gttttttgcc tgcaattgcg tcacgacatc ggtctctggt gctgcgcctt
     1141 cagcgcttgc ggtcgtcagt gcacttgaca cgccaccgcc agcttgcgcg gtctgcgcca
     1201 cctcctggcg ccgaaagcca gcggtgttca tgttggcaat gttgtgtgcc gaagaattca
     1261 gcgtcacctg ggctgcgttc atgcctgaga gcgcaattga cgaaatggaa gccatttgaa
     1321 accccgcgtt ggtggtgaga tggtcatgat ttgatcacaa acagtgcgca acttgtcaag
     1381 ttgatctaca tccaaaattt gtagataaaa aaggcaaatg atgcgagttt caagatgagg
     1441 caactcgttg gcagacagat ttcctgctgg cttggtgcaa acgcttgacc tgaaagagga
     1501 tgacgaaatt gatcttcacc ttgtcggtgc ccaatttctt gaggccgtca agagaccgtc
     1561 caccaaagaa ctgcttgcac gctcgcaccg gtttcttcgt cgattgccag cggcttttca
     1621 ttttgatcgg ctgcacgcca atgaaaggtg gtatggcagg tagtgaacaa gccaatgacc
     1681 cagcttaccc tcgaactgtc ttttcaaggc agacaccgat cacaaatgtg ccgacactga
     1741 atcaatggtg taatatcttg ctgtgttatt acatgaatca ttcaatgacc atctctgcca
     1801 agttattcat gagtggccgc agtcaagcaa ttcggctgcc ggccaagctg cgcctggctg
     1861 ccaaagtagt tctgattgag cagattggcg atgggctttg gattcgccct gaacctgaac
     1921 caactcaaaa catgggggtt tggctgcaaa gcttctatga caacacacag gcactacccg
     1981 aagatttttt agctgaacgt cacgacgcac cagcgcaagc acgcgactgg gtcttggact
     2041 gacaggcgct gcaccatgct gcgcacgctc gataccaata tctgtagcta tgtgctgcgc
     2101 cgccgcccgg ctgccatgct ggagcgattt gctgggctga accgacagca gctttggctc
     2161 tctggcatcg tggcggcgga actnnnnnnn nnngcggaac tgcgtcttgg cgctgccaag
     2221 ctgggtgcgc cccgttttac gaaagcggta gagacttggc tggcaggctt cgacgtgcaa
     2281 gcctggccgc tgcaagccac cgaacactac gcgcacattc gagccaaact ggagcatgac
     2341 ggcaacatct ttggggcgat ggatttgctg attgcttcgc atgcactggc caacgactcc
     2401 gtgctggtca ccaacaatgc acgggaattt gcccgtgtgc cgggcttggc tgttgaagaa
     2461 tggcaattgg aatgaacaaa gtggtgactc atttccaggc gcagacccac ccatgcagtt
     2521 agtccgtgac ctgaccgctg cgatgtcaga caacgtcggc caagctggcg acgtgggcta
     2581 caccgtcatc aactacaaca ccgccgccca aacccttcgc tgcattgagt cgctggcgca
     2641 ctgcacgccg ccgcctgcgt gggtcgtcgt gctggacaac gcatcagcag aagctgactt
     2701 tgacaagctg aaaagcggtt tgcggcgtgc cgaccaatgc catattcgac ttttccgcag
     2761 tgatgtcaac cttggctttg ctgcgggcag caactttttg attgaccagt tgctcgacat
     2821 ccctgcctgc cagtacattg gcttgctcaa caacgatgcc gttgccaagc ccgagctggt
     2881 tcagcttcta cgcgccgcat tgcttgaaac cacccactcc attggcatgt cgggcgggcg
     2941 catgcacaag ctgcaccatc cagaagaggt ggacaccctg ggcatcagtt tgtatgccag
     3001 cctgatgccg gctgatcgca aagccatctc agacccctat cttggcccca ccggtggctg
     3061 ctgcctgctg acgcgtgatt ttgtgcagga tgtcaaagcc accactggct attgctttga
     3121 cgcgcggttt ttttgctact gtgaagacac cgatctggcc ctgcgcgcca acctgctggg
     3181 ctaccggcca gcgtatgtgg accaactggt cgccttgcac gagggccagg ccagcgccaa
     3241 gacggccaac aacaatttca ttgcgtacca tggcctgcgc aacaccatgt ggatgcaatg
     3301 gaagtgtctg ccctcgtcga tactgctgac gcatgcgccg tggttgttgc tggcgcactt
     3361 gctcacattc actcgccaat tattttctgg tcgatctggc ctaatgttga aggtgtatcg
     3421 agatgctctt aatctgctgc cttcggtcaa gaccgagcgc gttcagctaa aagccagttc
     3481 gagggttccc agcgcatcgc ttgcagcaca tttcagccga cgtttctacc gcaaggggta
     3541 catctgcgtg gtactgtctg agtggaaggc acggctatca attgcagggc gcatctgact
     3601 gattggtaga gtctccaatg caccattaaa atcgcccctt tgctgcacct ggaacagcct
     3661 accaacccga gcgtttcttt gaattcaata gcgattaacc cccatgcagc acaaccgact
     3721 tcgctgatgt cgttaggcaa gagcctatgg cgcaataggc agctcattgc gcaaatgacc
     3781 cggcgcgatg tggttggccg ctacaaaggt tctgccatgg gcttggcatg gtcatttttt
     3841 aacccagtgt tcatgctggt ggtttatacc ttcgtttttt cggtcatttt caaatcgcgt
     3901 tggggcggtg ctggtggtga cgacagcaaa acccagtttg cggtcgtgtt gtttgttggc
     3961 atgattgtga tgagcctgtt tagcgatgtg cttaaccggg caccaagcct tatcctgagt
     4021 aacgtcaatt acgtcaaaaa ggttgttttc ccgctggaaa ttttgccggt gattgccaca
     4081 ggggcagcag tttttcatag catgatcagc ttgggcgtgc tgctggtcgc atttgtgctg
     4141 ttcaacggct accttcactg gactctggtt tttgtccccc tggtgatgct gccattggtc
     4201 attttggcgt tgggttttgc ctggatgctc gcatcattag gggtgttttt acgggacgtg
     4261 ggccaatttg tgggcatcgt gaccacagta atgatgtttt tgtcgccagt gttttacccg
     4321 gtcaccgcgg tgcctgaacg
//
LOCUS       DAOSLL010000417         4316 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_17997_length_4316_cov_4.323633, whole genome shotgun sequence.
ACCESSION   DAOSLL010000417 DAOSLL010000000
VERSION     DAOSLL010000417.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4316)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4316
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17997_length_4316_cov_4.323633"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..540)
                     /locus_tag="PLB25_15990"
     CDS             complement(37..540)
                     /locus_tag="PLB25_15990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43111.1"
                     /translation="MSDLPVTLKRIHAQRLREVYRSAGWPYQDVVEIDLLAAGLLERV
                     TTDSGHAVVRVTDAGIGHLAIATQGNRLARTAHETLVEKVVESMLRDGRIAWTGLSLR
                     AKVAGADADDTYWKMCQRDVFSIRNISRHDYLEPVVHEIRVSRADLLGDLKRPEKLAE
                     YLELIRK"
     gene            complement(544..1846)
                     /locus_tag="PLB25_15995"
                     /pseudo
     CDS             complement(544..1846)
                     /locus_tag="PLB25_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003069338.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
     gene            complement(1858..2244)
                     /gene="umuD"
                     /locus_tag="PLB25_16000"
     CDS             complement(1858..2244)
                     /gene="umuD"
                     /locus_tag="PLB25_16000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011458711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HOE43112.1"
                     /translation="MVSVLPCRVAAGFPSPAEDHAVQRVDLMAQLIKHPQATFLLRVR
                     GESMKDAGIFDNDVVLVDRAITARSGHIVIAMVDGGFVCKTLRQRSGRMKLKAANATF
                     PDINPADGQTVEIWGVVVASIKQFKA"
     gene            complement(2838..3023)
                     /locus_tag="PLB25_16005"
     CDS             complement(2838..3023)
                     /locus_tag="PLB25_16005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43113.1"
                     /translation="MSLSNGGVDSSTSTVSLTISPVNDVAVVTDVAGIDRTLNEGSTL
                     TLDLLIHADGSVNLLSS"
     gene            3092..>4316
                     /gene="glgX"
                     /locus_tag="PLB25_16010"
     CDS             3092..>4316
                     /gene="glgX"
                     /locus_tag="PLB25_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HOE43114.1"
                     /translation="MNHLPFSEVSVGRPSPLGATLDANGVNFALFSAHADRVELCLFD
                     SSGKHELQRVELRARTNDIWHGYLPQARADLLYGYRVHGRYRPQEGHRFNAHKLLVDP
                     YAKSLAGEIEWSDAHFGYRIGQSTADLSLDQRDNARLMPKCRVIDPAYNWEDDRPPRT
                     PWKDTVIYELHVKGFTIAHPSVPPELRGTYAGLASAPVIEHLKRIGVTAVELLPIHAF
                     AQDRHLVERGLRNFWGYNSLAFLTPQPSYCATGSISEFKTMVKALHAAGIEVILDVVY
                     NHTAEGNQMGPTLSMRGIDNASYYRLVTGNARYYMDYTGCGNTLDTSNSHVLELIRDS
                     LRYWVEDMHVDGFRFDLGVALTRDMTGVEMRGAFLQEIARDPVLSQVKMIAEPWDLGP
                     GGYQLGCFPAGWGEWN"
BASE COUNT          925 a         1269 c         1184 g          938 t
ORIGIN      
        1 tcatggcatt tcgcaaaatc aagttaggtg taaacattat ttacggataa gttctaagta
       61 ctccgcaagc ttttcaggcc gcttcaaatc tcccagtaga tcagcgcggc tgactcttat
      121 ttcgtggaca acgggttcca gatagtcatg ccgggaaatg ttgcgaatgg agaagacatc
      181 gcgctggcac attttccaat aggtgtcatc ggcatcagca cctgctacct tggcacgcaa
      241 gctcagcccc gtccaggcga tccgtccatc gcgcagcatc gattccacca ccttttccac
      301 caatgtctcg tgcgccgtgc gcgccagacg gttcccctgc gttgcaatgg ccagatgccc
      361 aatgccagcg tctgtaactc gtaccaccgc gtgaccgctg tccgtggtga ctcgctccag
      421 caatccggca gccaaaagat cgatctccac cacatcttga tatggccaac ccgcagagcg
      481 gtagacctca cgcagccgct gggcgtgaat ccgcttgagt gtgactggca gatcactcat
      541 ggtttatgcc ctcaccaacg ccagaccatc ccagtccgtg gtgtacccag gcgtgcgccg
      601 ttcctgtttc atgctccata gccgctgctc ccccgccagt ccagcgctgg ccagagccac
      661 cgtcccgcgt ccatacctct gattcaccgc atccagtgcc agcatcagcc gacttccgcc
      721 agggtcagcc acatcgtctt ccagcgccaa ttcttgctgg accaagtctg cgggccgcag
      781 atcaaacaac atcacacctg ctttgggata gcggtatcca ggtcgaaaaa ttgcctgcaa
      841 gcccagcagt gcagcttggc tgatgtcccg actgtcgttg gttgatcgcc tgagcggcac
      901 caccgttgac cgcgaatact gcaaatcttg cgtccgaaac gggctagtgc ggataaacgt
      961 gagcacctgt ccggcattgc agttttgcag tcgcaatttc tgcgcagccc gactggcaaa
     1021 ttccgtcacc gcttcctgta aggcggacaa gtccaacacc gcatggcaaa agctgcgtgt
     1081 gcaggcgatc acttgtttcg ctggtggctc gtgctccaga cccatgcagt cggtaccttg
     1141 cagttcacga acggtacgtt ccagcaccac agaccagcgg cgtttgacca tgaccggatc
     1201 aagtcgcgcc aggtcaagca cggtatggtt gccagcctct ttaagttgtg caccgatgcg
     1261 tctgccaacc ccccaaacct ccccaacctc cgtggccacc atcaatgcat cgcgctctgt
     1321 cggctccaaa ttgccgggat tgcacacctg ggagtgatga tcggggtagc tacccggttt
     1381 gcgttcagcg gttttggcaa tgtggttggc cagcttggct aaggtcttgg tggaaccaat
     1441 cccgatcccg caggcgatgc caatccattt gaggatgcgg tgcctgattt ttcggctgcg
     1501 catggtcatg tcgcccgtga tgccggtcag gtcaataaac gactcgtcaa tcgagtacac
     1561 ctcctggcgg tgccccagcc ctgcggcaaa actcatcatg cggtcgctca tgtcgccgta
     1621 gagtgcaaaa ttggccgaca acgccactag tccgtctgat tcctcgaaat ggcgaatttg
     1681 aaaccagggc gcgcccatct tgatgcccag cgccttggct tcgtttgacc tggcaattgc
     1741 acagaccgtt gttggacagc accactaccg gcctgccatt cagactcgga cgaaacaccc
     1801 gttcgcacga gacacaaaaa ttgttgccat cgaccagcgc gtacatggtc ttcccattca
     1861 ggctttgaac tgtttgatgg atgccaccac cacaccccaa atttcgacgg tttgtccatc
     1921 cgccgggttg atgtctggaa aagtggcgtt ggcagctttg agtttcatgc ggcccgatcg
     1981 ctgccgcagg gtcttgcaga caaatccgcc atcgaccatg gcaatgacga tgtggccaga
     2041 gcgcgcagtg atagctcggt ccaccagcac gacatcattg tcaaaaatgc cagcatcctt
     2101 catggattca ccgcgcacac gcagtaaaaa tgtggcttgt gggtgcttga tcaactgcgc
     2161 catcaagtcc acacgctgca cggcatggtc ttcggcagga gacggaaagc ccgcagccac
     2221 ccggcacggc aacacactga ccatagctgg gatcagcgag agtgcaacgg gttggtcaag
     2281 agggattgcg gtgaggccca ttaaatactg ctgttatata cagtatgtga taagacctca
     2341 acctttgtat cgcgaatgcg caatgtttga gcaaacctcg caagtccttg gctggcgcag
     2401 cttttgggac ttgttccgac agttgtccac agcgttgggc acggattttg ggaacaagat
     2461 gcggctacga tcaggcaatg tgcagtcact accaagccat caaggagcgt gaacgctacg
     2521 ccaaacactt cggcgttgtg ccgcccgttg acgcgggcaa acacgatgtc tgacgcgggt
     2581 tgtagagctt ggttttggcg cccgctggcc caccggcgtg cggtggcggg catggggcag
     2641 tgccagcagg cgctgggtgc ccgatgatat taaatttata tccagccaat tgcatttgtg
     2701 aaagcggcaa ggggcaccga atctgcgttg gggggacgag cgtccgtcac gtcccggttg
     2761 ctgtggaaat tgcctggtcg aattctcttt ttttttgaat cgtgctctag cctctgtgga
     2821 atatgcccga aatgatatta acttgatagc agattgacag agccgtccgc atggattagc
     2881 aggtccaggg tgagtgtgct gccttcattc agcgtgcggt caatcccggc aacatcggtc
     2941 accaccgcca cgtcgtttac ggggctgatg gtcaggctca cggtagaggt gctggagtcc
     3001 accccgccgt tgcttaggga catagcaatt gctaaagcgc gccaagcacg atccagtggt
     3061 gcaaaatttc aatcatagac tcggactccc aatgaaccat cttccatttt cagaagtgag
     3121 cgtcggcagg ccaagccccc tgggggcaac cctggacgcg aacggagtca actttgcctt
     3181 gttttcggct catgccgata gggtggaact ttgcctgttc gattcgagtg gaaagcacga
     3241 gttgcaacgc gtggagctca gggcgagaac caacgacatc tggcacggat atctgccaca
     3301 ggcgcgagca gatctcctgt acgggtatcg cgtccatgga cggtaccggc cacaggaagg
     3361 tcaccgcttc aatgcccaca agttgctggt tgatccttac gccaagtcgc tcgccgggga
     3421 gattgaatgg agtgatgccc actttggcta tcgcattggc cagagcacag cggacttgtc
     3481 actcgaccag cgcgacaacg cacgcctcat gccgaagtgc cgggtcatcg atcccgccta
     3541 taactgggag gacgaccggc cgccgcgcac accctggaag gatacggtca tctacgagct
     3601 gcatgtaaag ggcttcacga tagcgcatcc cagtgtgcca cccgaacttc gcggaacata
     3661 cgccggtctg gccagtgcgc ccgtcattga acatctcaag cgaatcggcg tgaccgcggt
     3721 cgaactgttg cccatccacg catttgccca ggatcgccat ctggtggaac gaggtctacg
     3781 caatttctgg ggctataact cgcttgcgtt cttgacaccc cagccaagct attgcgctac
     3841 gggcagcatc tcggaattca agaccatggt caaggcgcta catgccgctg gcattgaggt
     3901 cattctcgat gtggtctata accacaccgc agaaggcaat caaatgggcc cgaccctgag
     3961 catgcgcggt atcgacaatg caagctatta ccgactggtc accggcaacg cgcgttatta
     4021 catggattac accggttgcg gcaacacgct cgacacctcc aattcgcacg ttctcgaact
     4081 catacgagac agcctgcgct actgggttga ggacatgcat gtcgatggtt ttcggtttga
     4141 cctcggtgtc gcattgacgc gtgacatgac cggtgtcgaa atgcgcggtg cgtttctcca
     4201 ggaaatcgcg cgagacccgg tactgtccca ggtaaagatg attgccgagc cgtgggatct
     4261 cggtcctggc ggctaccagt tggggtgctt cccagcgggc tggggggaat ggaacg
//
LOCUS       DAOSLL010000418         4299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18097_length_4299_cov_4.717719, whole genome shotgun sequence.
ACCESSION   DAOSLL010000418 DAOSLL010000000
VERSION     DAOSLL010000418.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18097_length_4299_cov_4.717719"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PLB25_16015"
     CDS             complement(<1..334)
                     /locus_tag="PLB25_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE43115.1"
                     /translation="MANILVVDDELGIRDLLSEILNDEGHTVEVAENAAQARAARLRE
                     RPDLVLLDIWMPDTDGVTLLKEWSATGVLTMPVIMMSGHATIDTAVEATRIGALAFLE
                     KPITLQKLL"
     gene            complement(375..2666)
                     /locus_tag="PLB25_16020"
     CDS             complement(375..2666)
                     /locus_tag="PLB25_16020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE43116.1"
                     /translation="MNGVPRHSPTTITRNSRTLRWIIVLGLVAMVAVGLVLLFLLTQA
                     TTNRELYERNYTRLFIVNLVVASLLMLVICWIAYRLLKRLRQKKFGSRLLVKLAAVFA
                     LAGFAPGVLIYVVSYQFVSRSIESWFDVKVEGALEAGLTLGRTTLEALSSDLAGKARV
                     AATQLSETLDVSAALPLERARDAMGADDLTLWGASGRLIAASGQSRYQFSPELPSQQQ
                     FRAARAQRFITWVEGLDDTVGSESVDGRIKALVLVDSREFGSLSEPRYLLATKILPST
                     LVANALAVSQANREYQERALAREGLRRMYIGTLTLSLFMAVFGGVLLAVVLGNQIARP
                     LLLLADGVREVAAGDLTPKASLRGKDELDGLTRSFADMTQQLADARQAVQASMVEVNA
                     ARANLQTILDNLTAGVIVLDANGAVVSSNPGATRILRLPLAAFEGKRLGEIEGLETLG
                     TSVQQQFEAFLLPGRERNLDHWQQSFELGNSSTAQIGRPANDVITLIARGAELPDNQR
                     LLVFDDISEIVSAQRSQAWGEVARRLAHEIKNPLTPIQLSAERLEMKLSGKLGIPEQA
                     LLTKSVKTIVDQVDAMKRLVNEFRDYARLPAAELKAVDLNALALDVLNLYANENAPAL
                     VAAELDPQCPQIQGDAQQLRQVMHNLLQNAQDATQSADRADSLHPVVLKTQWKAASQR
                     VRLSVLDCGTGFQDHILKRAFEPYVTTKDKGTGLGLAVVKKIADEHGSRVEIVNRLVD
                     GQVVGAQVSLSFAINRGATTQPG"
     gene            complement(2663..3301)
                     /locus_tag="PLB25_16025"
     CDS             complement(2663..3301)
                     /locus_tag="PLB25_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HOE43117.1"
                     /translation="MIMTAFTTHCLREKRLNWLAWLLAAVMLCLATAATAQSGFQDSP
                     QLKIERMDDGFWLSTQVNFELPVVVEDALLKGIPIFFVAHAKLLRDRWYWINKTVASV
                     QRRMRLSYQPLTRRWRLSIAIGEAPDVSQGLALNQNFESLAEAMSNIRRISRWKIAEL
                     SDIEPGIRHVVEFSFLLDVSQLPRPLQIGTLGQSDWLISRTATQTLELESGK"
     gene            complement(3252..>4299)
                     /gene="rsmB"
                     /locus_tag="PLB25_16030"
     CDS             complement(3252..>4299)
                     /gene="rsmB"
                     /locus_tag="PLB25_16030"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HOE43118.1"
                     /translation="NARYDAFTLVNQSVEAAKKMPGTRSQVAFINACLRRFLREHNAL
                     LALTDQNPVARWNHPQWWIEQLQADWPVHWEAILAAADQPGPMVLRINVRRTTQAEYL
                     LRLQAAGLGARPIGPCGIVLAEPCAVGQLPGFAEGWVSVQDSAAQMAAPLLLAGMRDK
                     PAPRVLDACAAPGGKTAHILELADVTVVALDLDPLRCDRIHQNLARLELDAEVVVADA
                     ADVSAWWDGEPFDAILLDAPCSASGIVRRHPDIRWLRRSTDLPQLTAIQSRLLTRLWP
                     LLKPGGRLLYCTCSVFRAEGVRQISAFLARTTQAALLDSPGHLLPVAGSKSVDNQSDD
                     HDGFYYALLERKAS"
BASE COUNT         1030 a         1258 c         1144 g          867 t
ORIGIN      
        1 tcagcaactt ttgcagggta ataggctttt ccagaaacgc aagggcacca atgcgggtgg
       61 cttcgaccgc tgtatcgatg gttgcgtggc cgctcatcat gatgaccggc atagtcaaga
      121 ccccggttgc cgaccactcc ttaagcaaag tgacaccatc ggtatcaggc atccaaatat
      181 ccaacaggac aaggtctggg cgctcgcgca agcgggccgc ccgtgcctgc gcagcatttt
      241 ctgcgacctc aacggtgtga ccttcatcat tgagaatctc agacaaaaga tcccgaatgc
      301 caagctcgtc atccaccacc aaaatatttg ccatagtgtc gtgcgcccca aaaaattatc
      361 attgctgtga tgggctagcc tggctgggtt gtcgcaccac gattgatggc gaatgataac
      421 gacacttgag caccaacgac ctgtccatct accaacctgt tgacgatttc caccctagaa
      481 ccatgttcat cagcgatttt tttgacaact gctaagccca gcccagtgcc tttgtccttg
      541 gtggtgacat acggttcaaa tgcccgcttg agaatatggt cctgaaagcc ggtaccgcag
      601 tccagcacgc tcaaacgaac acgctgtgag gcagccttcc actgagtttt caaaactacc
      661 gggtgcaggc tgtctgcgcg atcagcactc tgtgttgcat cctgagcgtt ttgcaacaag
      721 ttatgcatta cctgacgcag ttgctgggca tccccttgta tttggggaca ctgcggatcc
      781 agctctgcgg caactaacgc cggggcgttt tcattggcat aaagattaag cacatccagt
      841 gccagagcgt tgaggtcaac agctttgagt tctgcagcag gcaagcgggc gtaatcacga
      901 aactcgttga cgaggcgttt cattgcatcg acctgatcta cgatggtctt gacggatttg
      961 gtcaacaacg cttgctcagg gatgcccagc ttaccggaca gcttcatctc caggcgctct
     1021 gcagagagtt ggataggggt gagcgggttc ttgatttcat gcgccagacg ccgcgccact
     1081 tcaccccagg cctgggagcg ctgggcggac acaatttcag agatatcgtc aaagacaagc
     1141 aagcgctggt tgtctggaag ctcggctcct cgcgctatca gggtaatgac atcgttggcc
     1201 ggtcggccaa tctgggcagt actgctattg cccagttcaa acgactgttg ccagtgatcc
     1261 aggttgcgtt cacgtccagg gagcaaaaac gcctcaaact gctgctgaac actcgtgcca
     1321 agtgtttcca agccctcaat ctcccccagc cgcttacctt caaatgcagc cagtggcagc
     1381 ctgagaatac gggtggcacc ggggttggat gacacaacgg caccattggc atccagcacg
     1441 atcacgcccg cagtcaagtt atccagaatg gtttgtaaat tggcacgtgc ggcattgacc
     1501 tccaccatgc tggcttggac tgcctgacgc gcatcggcca attgttgggt catgtcagca
     1561 aaagaccggg ttaacccgtc aagttcgtct ttgccacgca atgacgcttt gggagtcaga
     1621 tcgcctgctg ccacttcgcg cacgccgtcg gccaacagca gcagcggccg cgcaatctga
     1681 ttgcccagta ccacggccag cagcacgcca ccaaaaaccg ccatgaacag gctcagggtc
     1741 aaagtgccga tgtacattcg gcgcaagccc tcacgcgcca atgcgcgctc ctggtactct
     1801 cggttggctt gagacaccgc cagcgcgttg gcgaccaatg tcgaaggtag tattttggtc
     1861 gccagcagat agcgtggttc ggacaacgaa ccaaactcgc gactgtcaac caacaccaat
     1921 gccttaatgc ggccatcaac ggactcagag ccgacagtgt cgtcgagacc ttcaacccaa
     1981 gtgatgaagc gctgcgcgcg agccgcccga aactgttgtt gtgaagggag ttccgggctg
     2041 aactggtatc gtgattggcc cgacgctgca atcaagcgac cagaagcacc ccaaagcgtc
     2101 aagtcatctg cccccatggc atcccttgca cgttctagcg gcagcgcggc gctgacatcc
     2161 agcgtttccg acaactgggt ggccgcgacg cgcgctttac cagccaagtc agaagacagc
     2221 gcttccagcg tcgtacgacc aagggtcaag cccgcctcca atgcgccttc gaccttgaca
     2281 tcaaaccagc tttctatcga gcgcgacaca aactggtagg acaccacata gatcagtacc
     2341 cctggggcga atccggccag ggcaaacaca gcggccagtt tgaccagcag acggctacca
     2401 aacttcttct gtcgcagacg tttgagcaac cggtaggcaa tccagcaaat caccagcatc
     2461 agcagtgatg ccacgaccaa gttgacaata aacagccggg tgtagttgcg ctcgtacagt
     2521 tcccggttgg tcgtagcctg cgtcaacaga aacagcaaca ccagaccaac cgccaccatg
     2581 gcgaccaaac ccaacacaat gatccaccgc agtgtgcgcg aatttctagt tatggtggtc
     2641 ggactatggc gagggacacc attcacttgc cggactccaa ctcaagtgtt tgggttgccg
     2701 tgcgggaaat tagccagtca gactgaccga gcgtgccaat ttgcaacggg cgcggcaatt
     2761 ggctcacgtc aagcagaaat gaaaattcaa ccacatgcct gattcctggt tcaatgtcgg
     2821 aaagctcagc gattttccag cgtgagatgc gccggatgtt tgacatggct tcagccaatg
     2881 attcgaagtt ttgattcaat gcgagcccct gcgatacatc tggggcctca cctatggcga
     2941 tactgagccg ccagcgacgc gtcaatggct gataagaaag gcgcatgcgt ctttgaacag
     3001 aagcaactgt cttattgatc cagtaccagc gatcacgcaa taacttggca tgggcaacaa
     3061 aaaaaatggg aatccctttg agcaaagcat cttcgacgac aaccggcaac tcaaaattga
     3121 cttgggtcga tagccaaaag ccatcgtcca tgcgctcgat tttcagttgc gggctgtcct
     3181 gaaaccccga ctgggcagtt gcagcggtcg ccaggcacag catgaccgca gcaagcagcc
     3241 aggcaagcca gttaagacgc ttttctctca agcaatgcgt agtaaaagcc gtcatgatca
     3301 tctgactgat tgtccacgga ttttgagcca gcaactggca gcaaatgccc gggagaatcc
     3361 aacaatgccg cctgcgtggt gcgggcaagg aaggccgaaa tttgccgcac cccttcggcc
     3421 cgaaataccg agcaagtgca atacagcaag cgaccaccag gttttagcaa aggccataat
     3481 cgggtcaaca gccgtgattg aatggcagtt aactgtggca gatcggttga ccgacgcaac
     3541 cagcggatgt caggatgcct gcggacgatt cccgaagcgg aacagggagc atcgagcaaa
     3601 atggcatcaa acggctctcc gtcccaccat gctgacacgt cggcggcatc agcgacaacc
     3661 acttcggcat ccaattcgag tcgggccaga ttttgatgaa tccggtcgca acgcagggga
     3721 tcaagatcca atgcaaccac agtgacatct gccagttcca atatatgcgc agtctttcca
     3781 ccaggcgcag cacaggcatc gagtacccgc ggtgctggct tgtcacgcat gcccgccagc
     3841 aacaaaggtg cggccatttg tgcagcactg tcttgtaccg acacccaacc ctcggcaaag
     3901 cccgggagct gcccaaccgc gcagggttct gcaagcacga tcccacaggg cccgattggt
     3961 ctggccccca atcccgcagc ttgcaagcgc agcaggtact cggcttgtgt ggtgcgccgg
     4021 acattgatgc gcaacaccat cggaccgggc tggtcggccg cggccaaaat ggcttcccaa
     4081 tggactggcc agtcggcctg aagctgctca atccaccact ggggatggtt ccagcgggcc
     4141 acaggatttt gatcggtcag tgccagcagg gcgttgtgtt cgcgcagaaa ccgcctaagg
     4201 caagcgttga tgaatgccac ctgcgacctg gttccaggca tcttcttggc tgcctcaacc
     4261 gactggttaa ccagcgtaaa ggcatcgtac cgggcgttg
//
LOCUS       DAOSLL010000419         4294 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18125_length_4294_cov_4.005662, whole genome shotgun sequence.
ACCESSION   DAOSLL010000419 DAOSLL010000000
VERSION     DAOSLL010000419.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4294
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18125_length_4294_cov_4.005662"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..200)
                     /locus_tag="PLB25_16035"
     CDS             complement(<1..200)
                     /locus_tag="PLB25_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007848200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl CoA-acyl carrier protein transacylase"
                     /protein_id="HOE43119.1"
                     /translation="MKSFAFVFPGQGSQSVGMLDAWGEHPQVREALGEASDALGEDLG
                     LLIKDGPKEALALTVNTQPVMLV"
     gene            complement(261..1238)
                     /locus_tag="PLB25_16040"
     CDS             complement(261..1238)
                     /locus_tag="PLB25_16040"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HOE43120.1"
                     /translation="MKIYSRITGTGSFLPPRRLTNADLVAELAAQGTESNDEWIVERT
                     GIRARHFADDGMFCSDMALHASRAALQAAGIDAKDIDLIIVGTSTPDMVFPSTACILQ
                     GKLGVRGGAAFDVQAVCSGFIYALTVADAMIKSGAATKALVIGSEIFSRILDFKDRTT
                     CVLFGDGAGAVVLEASEQPGILATDIHADGKYTDILCVPGHVSGGQILGEPLLRMDGQ
                     AVFKLAVGVLDEAARAVLAKAGKTASEVDWLIPHQANIRIMQSTARKLKMPMDKVVIT
                     VDQHGNTSAASIPLALDTAVRSGQVKAGQTLLLEGVGGGFTWGAVLLTL"
     gene            complement(1242..2303)
                     /gene="plsX"
                     /locus_tag="PLB25_16045"
     CDS             complement(1242..2303)
                     /gene="plsX"
                     /locus_tag="PLB25_16045"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HOE43121.1"
                     /translation="MITISVDCMGGDHGPRVTLPACTEFLDQHPEAALVLVGLRDGLE
                     GYSHPRARLLFASEVVTMDDPLEVALRRKKDSSMRVAIAQVKDGEAQAAVSAGNTAAL
                     MAISRYLLKTLDGIDRPAIAGQIPNARGLGTTVLDMGANVDCAAEHLVQFAVMGSALV
                     SVLSGNENPTVGLLNIGEEAIKGNEVIKKTGELLRAASAAGDLNFYGNVEGNDIFKGT
                     VDIVVCDGFVGNVALKASEGLAGMIVDFLRKEFSRSIFTKMAALAAYPIIAALKKRMD
                     HRRYNGGALLGLRGLVFKSHGSADALAFSFALARAYDAARNNLLEQVRARIAHAAPLL
                     VPSGVPVGVANDPVAIALV"
     gene            complement(2435..2617)
                     /gene="rpmF"
                     /locus_tag="PLB25_16050"
     CDS             complement(2435..2617)
                     /gene="rpmF"
                     /locus_tag="PLB25_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HOE43122.1"
                     /translation="MAVQQNKKSPSKRGMHRAHNALVVPGIAIEATTGETHLRHHISP
                     TGFYRGRKVLKTKAQA"
     gene            3486..>4294
                     /locus_tag="PLB25_16055"
     CDS             3486..>4294
                     /locus_tag="PLB25_16055"
                     /inference="COORDINATES: protein motif:HMM:NF013166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HOE43123.1"
                     /translation="MRKGITPWLAYCTPRPDAPIRLFCFPYAGGGAAFYRNWSSELAP
                     EVDVCPVQLPGRETRMREPAYEELGPLVEQASTALLPYLDRPYCFLGYSMGTLIAFEL
                     ARHLDRHFQTRPSHLIACAFGAPHLPHISRLRYDLPEDEFIYELGKLEGTPQEVIECR
                     ELLDLVLPTIRADCRICDRYKFLKGELLPFPISAFGGIDDPQAGQELMQGWGDMTNST
                     FSLRMFPGRHFFIHESRRQFMSALESVLMGHVIVNWTVPSSPRYSGGFLARR"
BASE COUNT         1006 a         1253 c         1135 g          900 t
ORIGIN      
        1 accagcatca ccggctgggt gttgaccgtc agcgccagtg cttctttcgg gccgtccttg
       61 atcagcaagc ccaggtcttc acccagcgca tcggaggctt ccccgagtgc ctcacgcacc
      121 tgcggatgct caccccacgc gtccagcatg ccgactgact gcgaaccctg acccggaaaa
      181 acaaaagcga atgatttcat gttttgataa aaaattaaag gcaaacgggc atctagagcg
      241 catgcaatat gcgctggcag ctataatgtt agtagcactg caccccatgt aaagccgccg
      301 ccaacacctt caagcagcaa ggtttgtccg gctttgacct ggccgctgcg tacggcggta
      361 tcaagcgcca gcggaatcga tgcggccgag gtattgccat gctggtcaac cgtgatcacc
      421 accttgtcca tcggcatttt gagcttgcgg gcggtgcttt gcatgatgcg gatattggcc
      481 tggtgcggaa tcagccagtc cacctcggag gcagttttgc cggccttggc cagcaccgcg
      541 cgcgccgctt cgtcgagcac gcccaccgcc agcttgaaca ccgcctggcc gtccatgcgc
      601 agcagcggtt cgcccagaat ctgccccccc gaaacatgtc caggcacgca caatatgtcg
      661 gtgtacttgc catcggcatg gatgtcggtg gccagaatgc ccggttgttc ggaagcttca
      721 agcaccacgg caccggcacc atcgccaaac agcacacagg tggtgcggtc cttgaagtcc
      781 agaatgcgcg aaaaaatctc cgagccaatc accagcgcct tggtggcagc gccagacttg
      841 atcatggcat cggcaaccgt cagcgcgtaa ataaagccgc tgcaaaccgc ctgcacgtca
      901 aacgccgcgc caccgcgcac gccgagcttg ccctgcaaaa tgcaggcggt agacggaaac
      961 accatgtcag gggtggaggt gccgacaatg atcaggtcaa tgtctttggc atcaatgccg
     1021 gccgcctgca gggctgcgcg cgaggcatgc agcgccatgt cactgcagaa catgccgtcg
     1081 tcggcaaaat ggcgcgcccg aataccggta cgctcaacaa tccattcgtc gttcgactcg
     1141 gtgccttgcg ccgccagttc ggccaccaaa tcggcattgg tcaaacgccg tggcggcaaa
     1201 aaactaccgg tgccggtgat gcgagagtaa attttcatag ttcagacaag tgcaatggct
     1261 actggatcat ttgccacgcc aaccggcacc ccagagggca ccaacagcgg ggcggcgtgg
     1321 gcaatgcgcg ccctgacctg ttccagcaaa ttatttcggg ctgcatcata cgcccgcgcc
     1381 agggcaaaac tgaaagccag cgcgtcggcc gacccatggc tcttgaacac caggccacgc
     1441 aaccccagca aggcaccgcc gttgtaacga cggtggtcca tgcgtttttt tagcgccgct
     1501 atgatcggat aggcggccag cgccgccatt ttggtgaaga tgctccgcga aaactctttt
     1561 ctcaagaaat cgacaatcat gccagccagg ccctcgctcg ctttcagcgc cacgttgcca
     1621 acaaagccgt cgcacaccac aatgtcaacg gtgcccttga aaatgtcatt gccttcgacg
     1681 ttgccgtaaa aattcaggtc accggcagca gatgcggcgc gcaacagttc gccagttttt
     1741 ttgatgactt cgttgccctt gatggcttct tcgccaatat tgagcaggcc gaccgtcggg
     1801 ttttcattgc cgctgagcac cgacaccagg gctgagccca ttaccgcgaa ctgcaccaga
     1861 tgctcggccg cgcaatcgac attggctccc atatccaaca ctgtggtgcc caaaccccgg
     1921 gcattcggaa tctggcctgc aatggcaggg cgatcaatgc catcgagggt tttcagcagg
     1981 tagcgcgaaa tggccatcaa ggcagcagtg ttgcccgccg acactgccgc ctgcgcctca
     2041 ccgtccttga cctgcgcgat ggccacgcgc atggacgagt ccttcttgcg ccgcaacgcc
     2101 acctcaagcg ggtcatccat ggtgaccacc tcgctggcaa acaacaggcg cgcgcgcggg
     2161 tgggagtagc cctccaaacc gtcgcgcagg cccaccagaa ccagtgccgc ttcggggtgc
     2221 tggtcgagaa actcggtgca ggccggcaag gtgacacggg gaccatgatc gcctcccatg
     2281 cagtcaacag aaatagtgat catgaaaggc aaaatgacac agataacatc agaaaactcg
     2341 acagatccgg cgtacgggaa acagatgatg caaaaaaagc aaaagcccgg ggctacactt
     2401 ttggtagcct cgggctgtgg gaacctgact ggctttaagc ttgtgccttg gtcttcagta
     2461 ccttgcgacc tcggtaaaac ccggtcgggc tgatgtgatg acgcaaatgc gtttcgccgg
     2521 tggtggcctc aatcgcgatg ccgggcacca ccaaagcatt gtgcgcacgg tgcatgccgc
     2581 gcttggaagg tgactttttg ttttgttgga cggccatgct ggcactcctg aagacttgaa
     2641 accgcctgaa accaggcagc ccgggattgt tgaacggcgc aagtctgact tgcgcgaagg
     2701 tggcagagta taacttattg ccgctgtctt gatgacctca agccttgtcc aacgaggtgt
     2761 gagcctgaac ggctagccta tttccaccca gagtgagatc aaagcgggtt gagcaactca
     2821 ccaaattgcc aacaggctga tcatccaagg atggagatca acatgaacga accaaagctg
     2881 cgcacgatta cccctgattc agaatagttg tcgctcccaa aaattgcgaa tgatccgcat
     2941 caaagcgcat tgagcaccga aacctggcga accttgccac caatgcgctc gccgcgcacc
     3001 aggcaaaatg ggaaatagcg ttcacctgtg gttttgttgt tggcacgggt ctgtcaaatg
     3061 aacatgcttg ccgtgctggt ggtaaaaatc cggaagcagt cataattctg atggcacaac
     3121 aaaacgcaac caattccaga ccaccagcac tcccgcattt atcacatagc tatgttttta
     3181 atagcttttc aaaaacgggg cttacgatta ttttgtttgt tcatgaccgg ttgagcactt
     3241 gccaagcaat ccgatggtgc ccgccgcttc accgaaatac cgcgtgctac tgcatcgcca
     3301 cccgtaatgt caaattccac tcaaaacggc acaaagccct ataagttcaa tgcgcatgaa
     3361 tttcataatg caaaaggtca agatgctttg gcgtaactat tgtttgtgaa ttcggtgaca
     3421 acaatgcata ttcaaacaca tcaagcaaaa agttcatggt acgcagataa gccatggcga
     3481 ataacatgag gaaaggcatc accccatggc tcgcgtactg cactccacgg ccggatgcac
     3541 ctatcaggtt gttttgcttt ccctacgcag gcggcggcgc ggccttttac cggaactggt
     3601 catcagagct tgcacctgag gtagatgtct gcccagtaca gcttccaggc cgcgaaacac
     3661 ggatgcgcga acccgcttac gaggaactcg gtccgctcgt cgaacaagcg tcaaccgcac
     3721 tgttgccata tctggaccga ccgtattgtt ttcttggcta cagcatggga acacttatcg
     3781 cctttgagct ggcacgccat ctggatcgtc atttccaaac gcgtccaagt catttgatcg
     3841 catgcgcttt tggagcgccg catctcccac atatctcccg gttacggtat gaccttcctg
     3901 aggatgagtt tatctacgaa cttggaaagc ttgaaggtac accccaagag gtcattgaat
     3961 gcagggaatt gctggatctc gtacttccca caatcagggc tgactgccga atctgcgacc
     4021 gctacaagtt cctgaaaggt gaattactgc cctttccaat atctgcgttt ggaggcattg
     4081 acgatcccca agcaggtcaa gaattgatgc agggttgggg tgacatgact aactcgacgt
     4141 ttagcctcag gatgttcccg ggcaggcatt tcttcataca tgagtctcgt cgacagttca
     4201 tgagcgcatt ggagtccgtc ttgatgggtc atgtaattgt gaactggaca gtgccaagtt
     4261 ctccccgtta ttcaggcggt ttcttggcca ggcg
//
LOCUS       DAOSLL010000420         4276 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18223_length_4276_cov_4.215115, whole genome shotgun sequence.
ACCESSION   DAOSLL010000420 DAOSLL010000000
VERSION     DAOSLL010000420.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4276)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18223_length_4276_cov_4.215115"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(53..1519)
                     /gene="ccoG"
                     /locus_tag="PLB25_16060"
     CDS             complement(53..1519)
                     /gene="ccoG"
                     /locus_tag="PLB25_16060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HOE43124.1"
                     /translation="MKKPTHSRIIQIHAVPKTAGQADSSAPVLALHQDHSKIYAKAVG
                     GRFAKLRWIMVWVTQLVFYGTPWLRWHERQAVLFDLANSRFFIFGLVLHPQDLIYLAM
                     LLVLAALALFFFTAVAGRLWCGYACPQTVYTAIFLWLERLTEGDRVARMRLDGAHWGA
                     EKFARKSAKQALWVGLSLLTGFSFVGYFTPIHDLVTELLALSFSAWEWFWVLFYGLAT
                     YGNAGYLREQICKQMCPYARIQGALTDMDSMVIAYDTDRGESRGSRPRNADPKALGLG
                     DCIDCTLCVQVCPTGIDIRHGLQNECIACAACIDVCDAVMEKMNYPKGLIRYSSGKGM
                     DERSTPKQMVKRVWRPRVWVYGMLLLLVAALFIGGLSTRKGFAVDVIRDRGTMARETG
                     RGDIENVYRLQIMNTTEQVQTYTTSVQGLPGLVLATNPVLSVPAAGIGSLIIRLTLAA
                     EAAQGHRGEVSPILFRVETQGQAKVVSVAEKSTFVVPR"
     gene            1618..2097
                     /locus_tag="PLB25_16065"
     CDS             1618..2097
                     /locus_tag="PLB25_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HOE43125.1"
                     /translation="MRLTQWTDYTLRVLMYCAASLERTEPVTITEIAENHGISRSHLT
                     KIVQELAARGWLETTRGRGGGVRLLVNAEQVSLGDVVRATETDFAMVECFDPVQNQCR
                     LSTHCRLQSVLNQATQRFLAVLDGITLADLVSPTSIRGDLAKERLLQLVPGLPSVSA"
     gene            complement(2542..2664)
                     /locus_tag="PLB25_16070"
                     /pseudo
     CDS             complement(2542..2664)
                     /locus_tag="PLB25_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612190.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2715..2921
                     /locus_tag="PLB25_16075"
     CDS             2715..2921
                     /locus_tag="PLB25_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43126.1"
                     /translation="MVASGHGIPVLPKSALSYQSQSDRVMASPIKNPPRRRAALAWHR
                     SASAGGASANCGRRPHGQADLLAS"
     gene            complement(2934..3647)
                     /locus_tag="PLB25_16080"
     CDS             complement(2934..3647)
                     /locus_tag="PLB25_16080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018714583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE43127.1"
                     /translation="MPNISAQPLILIEDELDLLASLVPLPGAQLIELGCGAARLAADL
                     LLRHPDARVTGLEVDERQMAKNLAAPATIGLNFVNAGAQAIPFADGSFDGALMLKSLH
                     HVPMALMAQALAEVARVLKPGGWLYVSEPVYAGPLNEIMRLFNDEAVVRAAAQQALDA
                     ALQSGVWQADDERRFEMPVFFADFDTFEQRMMRPTYANHQIDDTLHAAIRQRFESYMG
                     PQGVSLTRPMHVRLLRKSA"
BASE COUNT          934 a         1251 c         1210 g          881 t
ORIGIN      
        1 cgtgcatccc aactccttgg tgcaaaacgg ggagttattc ccgaccacat ccttaacgcg
       61 ggaccacaaa ggtcgatttt tctgccactg acaccacttt tgcctggccc tgggtttcca
      121 ccctgaacag gatcgggctg acttcaccgc gatgtccttg cgcggcttcg gcagccaggg
      181 tcaaacgaat gatcagcgac ccgatgcccg cagcaggcac gcttaacacc gggttggttg
      241 ccagcaccag gcctggcaaa ccttgcacgc tggtggtgta ggtttgcacc tgttcggtgg
      301 tgttcatgat ttgcagacga tagacatttt cgatgtcacc tcgcccggtt tcgcgggcca
      361 tcgtgccgcg atccctgatg acatccacag caaacccctt gcgcgtggac aaaccaccga
      421 tgaacagggc tgccaccagc aacagcagca tgccgtagac ccagacccgt ggccgccata
      481 cccgcttcac catctgcttc ggggttgatc gctcgtccat tcccttgccc gatgaatacc
      541 gtatcaaacc cttgggatag ttcattttct ccatgaccgc gtcgcagaca tcaatgcagg
      601 cagcgcaagc aatgcactcg ttttgcaggc cgtgtctgat atcaatgccg gtgggacaga
      661 cctgcacaca caatgtgcag tcaatgcagt cgcccaagcc cagggccttg gggtctgcgt
      721 tgcgtgggcg tgatcctctg gactcaccac ggtctgtgtc atacgcaatg accatcgagt
      781 ccatgtcagt cagtgcacct tgaattcggg cgtaagggca catctgtttg caaatttgct
      841 cacgcaaata gccggcattg ccgtaagtgg ccaagccgta aaacagcacc cagaaccact
      901 cccaagctga aaacgacaag gccagcagtt cggtcaccag atcgtgaatg ggcgtgaaat
      961 agcccacaaa gctaaacccg gtgagcaagc ttaagcccac ccacagggct tgcttggcac
     1021 ttttgcgggc gaatttttcg gccccccagt gcgcaccatc gagacgcatg cgggccaccc
     1081 ggtcgccctc agtgaggcgc tcgagccaca aaaagatcgc ggtgtaaacc gtttgcgggc
     1141 aggcatagcc acaccacagt cgccctgcca cggcggtaaa gaaaaacagc gccagtgctg
     1201 ccagcaccag caacatggcc agataaatca ggtcttgggg atgaagaacc aggccgaaga
     1261 tgaaaaatcg gctgtttgcc aaatcaaaca gaaccgcttg ccgttcatgc caccgcagcc
     1321 agggtgtgcc ataaaagacc aactgtgtca cccagaccat gatccagcgc aactttgcaa
     1381 agcgcccacc aacagccttg gcgtaaattt tgctgtgatc ttgatgcaat gcgagtaccg
     1441 gcgctgaact gtcagcttgg cctgcagttt tcggcaccgc gtggatctgg atgattctgg
     1501 aatgtgttgg ctttttcata aacttcacat cccactcaaa agaagaatgt catgtgcatg
     1561 ttattttagc cggataatgt tcatgctgta tgaatcttta ttttcccgat tgaacctatg
     1621 cgcctgaccc aatggactga ctacaccctg cgtgtgttga tgtactgtgc tgccagcctg
     1681 gagcgcaccg agccggtgac gatcaccgag attgccgaaa accacggcat ttctcgcagc
     1741 cacctgacca aaatcgttca ggaactggca gcccgtggct ggctggaaac cacccggggg
     1801 cgtggcggcg gagtgcggct gctggtcaat gccgagcagg tcagcctggg tgatgtggtg
     1861 cgtgccacag agaccgattt cgccatggtg gaatgttttg acccggtgca aaaccaatgc
     1921 cgcctgagca cacactgtcg gctccaaagt gtgctcaacc aggctacgca gcgtttcttg
     1981 gcggtactcg atggcatcac gctggcggat ctggtttccc ctacctccat ccgcggtgac
     2041 ctggcaaagg agcgacttct acagttggtt cctggcttgc cgagtgtctc ggcttaagcg
     2101 ctttggtttt ttaatttgct tccctttaga tagcatgaga cgctttgcgg acaagcgctg
     2161 gagggctaaa ttggtctaag cagatcgaca ccgaaggctg ggaaacccaa ggttcagcaa
     2221 cctgatatga ccattctgga ggcccggaaa gcacgcttag ggtcaagcga gaataacttg
     2281 ggcaaaggag gtcgtgatgg caagtgcaat gcgttgttgc aaaccgcttg tgtagcaacg
     2341 caaggcacgg cgagataaca gcgacatgga tgattctgaa atggaattag ccctttctgc
     2401 aaggatctgc atacagtaat gtgcagccac aatgctggcc ttctgccaac gatccaatca
     2461 tgcccatggc acttgcagcc gatgattttt ctagcttcct cgcctggcgg aagttcaaga
     2521 ccattctggc tgacccgccg ttgagccaaa ctgatcaggg aactctgggc ggttgttctt
     2581 catggttttg aaatgggcct gcgaatacgg gttgtcatcg gaggtgcagg gacggctgtg
     2641 gcgcttgctg gcatcgagat cagccgcact gctacctcga cgcgtcagcg ccactgcatt
     2701 gagacatccg tgccatggtg gcctcgggcc atggcatccc ggtgctgccc aagagtgcct
     2761 tgtcgtacca gtcccaatca gaccgggtga tggcgagtcc gattaaaaac ccgccgcgca
     2821 ggcgtgcggc gctggcctgg catcgcagcg catcagccgg tggtgcaagc gctaattgcg
     2881 gccgtcgccc acatggacaa gccgacttgc ttgccagttg attgcgtcag cagctatgcc
     2941 gatttgcgca gcagacgcac gtgcatgggg cgtgtcagcg acacgccttg cggacccatg
     3001 tatgactcaa aacgctggcg gatggctgca tgcagcgtgt cgtcgatctg gtggttggcg
     3061 taggtcgggc gcatcatgcg ctgctcaaaa gtgtcaaagt cggcaaaaaa caccggcatc
     3121 tcgaagcggc gctcgtcatc cgcttgccac acgccggact gcagcgctgc atcgagcgcc
     3181 tgctgggctg cagcgcgcac cacagcttca tcgttgaaca gccgcatgat ttcgttgagc
     3241 ggcccggcgt acaccggctc ggacacatac agccagccac ccggtttgag cacccgcgcc
     3301 acctcggcca gcgcctgcgc catcagcgcc atgggcacat ggtgcagcga cttgagcatc
     3361 aacgcgccgt caaagctgcc atcggcaaac ggaatggcct gtgcaccggc attgacgaag
     3421 ttcagcccga ttgtggcggg tgcggcaagg tttttggcca tctggcgctc atcgacctca
     3481 agaccggtga cgcgagcatc cgggtggcga agcagcaggt ccgctgccag gcgtgctgca
     3541 ccacagccca attcaatcag ttgcgcgcca ggcagtggaa ccaaactggc aagcaggtcg
     3601 agttcatcct caatcaggat cagtggttgt gcggaaatgt tgggcattgt tgaacgtgac
     3661 aaaatggtag ggaatgcgcc aatcgtaccg ggcaattcgt catgcccggc caccgcacga
     3721 catcaagcta agacgcaaac gctggtgttt tggcagcctg gtcattggcg agtcggcatg
     3781 cgcagggcgg gcatcaccgc ctcaaccacc ccgtcagtcg aaggtgcgcc atggccatcg
     3841 ccatcaccag ccaaaacggc aaaactggtc gcagcgacaa tgatggctgg cctgccgcaa
     3901 gcggtttgat gcgtgctgac caaccatttg gccgcaaagc ctgcgggcat gacaagctgt
     3961 cgcgatctgc ctggcgctca gcggtcaggc ccgttggcgc tgaggatctg gtcgtgaata
     4021 actccccgtt ttgcaccagg aacttgggct gcacggccag gcgcaagacg cgccgtacaa
     4081 ctggctaagc aagcgccttg caacgccgcc gtgcgcccaa atccgccagt gcaagtgggg
     4141 gaagtttttt ctgaccacat cattgagcca gatcaaagcg gtccgcgttc atgaccttgg
     4201 tccaggcagc gataaagtct ttggcaaact tttcctggtt gtcgtcttgc gcatagacct
     4261 cggcgtaggc cctcag
//
LOCUS       DAOSLL010000421         4272 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18233_length_4272_cov_4.908466, whole genome shotgun sequence.
ACCESSION   DAOSLL010000421 DAOSLL010000000
VERSION     DAOSLL010000421.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18233_length_4272_cov_4.908466"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(177..2135)
                     /locus_tag="PLB25_16085"
     CDS             complement(177..2135)
                     /locus_tag="PLB25_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019448811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HOE43128.1"
                     /translation="MHTRLEDLKSTGARRNKVVSAAEAVRLIHDGDTVATGGFVGIGF
                     AEEVAVAIEAQFLQSQADDGQGSPKGLTLIYAAGQGDGQTRGLNHFGHVGLVRRVIGG
                     HWGLVPSLQELAINNQIEAYNLPQGVITHLFRDIAAGKPGTLTHVGLDTFVDPIFGGG
                     KINQCTTHDLVRRMQIDGRDYLFYQAMPIHVGIIRATTADPDGNLSMEREALTLEALA
                     IAMAAHNSGGIVIAQVERLAESNTLHSRSVKIPGVLVDAVVVATNPAYHMQTFSEAYN
                     PAYSGEIRVPMNLIEPMALSERKVIARRAALELRPNSVVNLGIGMPEGVANVAAEERL
                     SDLLTLTAEPGVIGGVPAGGLSFGAAVNAQAVIDQPNQFDFYDGGGLDVAVLGLAQAD
                     AQGNLNVSKFGTRLSGAGGFINISQNAKKVVFVGTFTAGKLQLQIADGQLNIEVEGLQ
                     HKFVQQVEHRTFSAREAIARKRQVVYVTERCVFRLAGSVEQPALELVEIAPGVDLQRD
                     VLAQMDFVPLISPQLKLMDAALFQPEPMGLRERLLETPLTQRLDLNREHRTLFINFEG
                     LSVDSAEDINAIETHVTHLLAPLGHRVAVVVNYDSFTILPPLLDAYTDMVQRLKRQFY
                     SRVTRYGTGGFLKARLEASGSPPLADNP"
     gene            complement(2145..3182)
                     /locus_tag="PLB25_16090"
     CDS             complement(2145..3182)
                     /locus_tag="PLB25_16090"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE43129.1"
                     /translation="MKKLLRDLSFLSGLRDRQAMDFALVKLVAQSDLWCFSVVRLLRA
                     VGPEDEQHWVTLGKLDRQHSIPERDRFWFDSSSLPRLSDFAQREAAIVTEEVVRSGHG
                     PFITVFPIDTQASVCSVLEVESAEPVSADTEALIDSVLHLYENLQGLLDYGERDTLTE
                     LLNRKTFDGAFLRAAQTQDEPPDPERPDRRLIQAASTYWLAVIDIDHFKQVNDNFGHL
                     IGDEVLLLLARQMRSNFRFHDQLYRFGGEEFVVLMRCADHPDALAVLERFRCQIEQHA
                     FPQVGRITISIGFAPLRDNDTPSGAFDRADKAVYYAKSHGRNQTCSFTALVEAGELVE
                     SSDGGEDIDFF"
     gene            complement(3416..>4272)
                     /locus_tag="PLB25_16095"
     CDS             complement(3416..>4272)
                     /locus_tag="PLB25_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HOE43130.1"
                     /translation="QKIHRAHTEPAAHEEILGLEHFDKVINVDQSPIGRTPRSNPATY
                     TGLFTPIRELMSEVPTARERGYGPGRFSFNVSQAAGGGRCEACEGDGMVKVEMHFLPD
                     VYVSCDVCAGKRYNRETLEVLYKGKNIAQVLDLTVEAAFEFFKAVPTIARKLHTLLDV
                     GLSYIRLGQAATTLSGGEAQRVKLALELSKRDTGRTLYILDEPTTGLHFADIKLLLKV
                     LHQLRDAGNTIVVIEHNLDVIKTADWLIDMGPEGGSGGGTVVGIGTPEALAANPASHT
                     GRYLARLL"
BASE COUNT          803 a         1313 c         1329 g          827 t
ORIGIN      
        1 tgccgccgca gcgcgccagc aagccctgca agcgcgtgcg atgctggctg cagcgcgcta
       61 gcagcgtggg gctacagtac cggcataaat aagtgccaaa taggcctcta tcccttttgg
      121 aatgtgcgct ggcagctata aaaacaatat caaaaagccg cttctgatgg ctggcttcag
      181 gggttgtcgg caagcggtgg tgaaccactg gcttccagcc gggccttcaa gaagccaccg
      241 gtgccgtaac gtgtcacccg gctgtaaaac tggcgcttga gacgctgcac catgtcggta
      301 taggcatcga gtagcggcgg caaaatggtg aaactgtcgt aattcaccac caccgccacc
      361 cggtgtccaa gcggtgccag caggtgggtg acgtgggtct caatcgcgtt gatgtcttcg
      421 gcgctgtcca ccgacaggcc ctcgaagttg atgaacagcg tgcggtgttc gcggttcagg
      481 tccagccgct gcgtcagcgg tgtctccagc aaccgctcgc gcaggcccat cggctcgggc
      541 tggaacagcg cggcatccat cagcttgagt tgcgggctga tcaaaggcac aaagtccatc
      601 tgcgccagca catcgcgttg cagatcaaca cctggcgcaa tttcaaccag ttcgagcgct
      661 ggctgttcga cgctgccagc caggcggaac acgcagcgct cagtcacata aaccacctgc
      721 cttttgcggg caattgcttc gcgggcgctg aaggtgcggt gttccacctg ctggacaaac
      781 ttgtgctgga ggccctcgac ctcaatgttg agctggccat cggcgatctg caattgcagc
      841 ttgcccgcgg tgaaggtgcc cacaaacacc actttcttgg cgttctggct gatgttgatg
      901 aagcctccgg cacctgacag gcgagtgccg aatttgctga cgttgaggtt gccctgggca
      961 tcggcctgcg ccagacccag caccgccaca tcgagaccgc cgccgtcata aaaatcaaac
     1021 tggttgggct ggtcaatcac cgcctgggca ttgaccgctg cgccaaaact caggcccccg
     1081 gccggcacgc cgccaatcac cccgggctcg gcggtcaggg tcagcaggtc agacagccgc
     1141 tcttcggcgg ccacgttggc aacaccttcg ggcatgccga tgcccaggtt gaccacgctg
     1201 ttgggcctga gttcgagcgc agcacggcgg gcgatgacct tgcgctcgct cagggccatt
     1261 ggctcgatca ggttcattgg cacgcggatt tcgcccgagt aggccgggtt gtaggcctcg
     1321 gaaaaggttt gcatgtggta ggccgggttg gtggcgacca ccaccgcatc gaccagcacg
     1381 ccgggaatct tgacgctgcg cgaatgcagc gtgttgcttt cggccagccg ctcgacctgg
     1441 gcgatcacga tgccgccgct gttgtgggcc gccatggcaa tggccagtgc ctccagggtc
     1501 agggcttcgc gctccatgct caggttgcca tcggggtcgg ccgtggtggc gcggatgatg
     1561 cccacatgaa tcggcatggc ctggtaaaac aggtagtcgc gcccgtcgat ctgcatgcgg
     1621 cgcaccaggt catgggtggt gcattggttg atcttgccac cgccaaagat cgggtcaaca
     1681 aaggtgtcga ggccgacatg ggtcaacgtg ccgggtttgc cagccgcaat gtcacgaaac
     1741 aggtgggtaa tcaccccttg cggcaggttg taggcctcga tctggttgtt gatggccagt
     1801 tcttgcagcg aaggcaccag gccccaatgg ccgccaatga cgcgacgcac caggccgaca
     1861 tggccaaagt ggttcaggcc gcgggtctgg ccgtcgccct gaccggcggc ataaatcaag
     1921 gtcaggcctt ttggcgaacc ctgcccgtca tcggcttgtg actgcaagaa ttgcgcctca
     1981 atggccactg ccacttcttc ggcaaagccg atgccgacaa agccgccggt ggccaccgtg
     2041 tcgccatcgt ggatcagtcg cacggcttct gctgcgctga cgactttgtt gcgccgggca
     2101 ccagtggatt tcaggtcttc gagtcgggtg tgcacaacat gttcctaaaa aaagtcaata
     2161 tcttcgccgc cgtccgacga ctccaccaac tcgcccgcct cgaccagcgc agtaaaactg
     2221 caggtctggt tgcggccatg gcttttggcg taatagaccg ctttgtcggc gcggtcaaaa
     2281 gcgccgctgg gggtgtcgtt gtcgcgcagc ggagcgaagc cgatgctgat ggtgatacgc
     2341 ccgacctgcg ggaacgcgtg ctgttcgatc tggcaacgaa aacgctccag cacagccagc
     2401 gcatcgggat ggtcggcgca gcgcatcagc acgacaaact cttcgccgcc aaaccggtac
     2461 aactggtcat gaaagcggaa attgctgcgc atttgccggg ctaacagcag cagcacctcg
     2521 tcgccaatca ggtggccaaa gttgtcattg acctgtttga aatggtcaat gtcaatgacg
     2581 gccagccagt aagtgcttgc tgcctgaatc agccgtcggt ccggccgctc ggggtcaggc
     2641 ggctcgtcct gggtttgtgc ggcgcgcaaa aaggctccgt caaaggtctt gcgattgagc
     2701 agctcggtca aggtgtcacg ttcaccatag tccagcaagc cttgcaggtt ttcgtacaga
     2761 tgcagcacgc tgtcgatcag ggcttcggtg tcagcggaca ccggttcggc cgactcgact
     2821 tcaagcacgc tgcagacgct ggcctgggtg tcgatcggga aaacggtgat gaacgggccg
     2881 tggccgctgc gcaccacttc ctcggtaacg atggccgctt cgcgctgggc aaagtccgac
     2941 aggcgtggca gcgagctgga gtcgaaccag aaacggtcgc gctcggggat gctgtgctgg
     3001 cggtccagtt tacccagggt gacccagtgc tgctcgtctt ccggaccgac ggcacgcagc
     3061 aaacgcacca cgctgaagca ccacaagtca ctctgcgcga ccagcttgac aagcgcgaag
     3121 tccatcgcct ggcggtcacg cagcccgcta agaaatgata agtcgcgcaa cagctttttc
     3181 atgacgctag tctaattgcg tttccaggtc gtcactactg aacccggctg gcctgttgca
     3241 agttggccca gcgcgcagca aacccatgca gtgccgatgc gccgatcaaa ttccagtcat
     3301 atcaggacgg tctggccaaa ccgttatggc gcactgtcgg cattttcaga ggcgacttgc
     3361 accgatccat cgccgcgcca ttgcaacagt cgcgctacta cggcgagtcc agcgattaca
     3421 gcaagcgcgc caggtagcgg ccggtgtggc tggccgggtt ggcggccagc gcctcggggg
     3481 tgccgatgcc aaccaccgtg ccgcccccgc tgccaccctc ggggcccatg tcgatcagcc
     3541 agtctgcggt cttgatgaca tcgaggttgt gctcgatcac cacgatggtg ttgcctgcat
     3601 cgcgcaactg gtgcaacacc ttgagcagca acttgatgtc ggcaaagtgc aggccggtgg
     3661 tcggttcgtc cagaatgtag agcgtgcgac cggtgtcgcg cttgctgagt tcgagcgcca
     3721 gcttgacgcg ctgcgcctcg ccacccgaca gggtcgtcgc cgcctgcccc aggcggatat
     3781 agctcaggcc gacatcgagc agggtatgca gcttgcgtgc gatggttggc acggccttga
     3841 agaactcaaa cgccgcttcc accgtcaaat ccagcacctg cgcgatattt ttgcccttgt
     3901 agagcacttc cagggtttcg cggttgtagc gcttgccggc gcagacatcg caggacacgt
     3961 aaacatcggg caaaaagtgc atttcgacct tcaccatgcc gtcgccctcg caagcctcgc
     4021 agcgcccgcc gcctgccgcc tggctgacat tgaagctgaa gcgcccgggg ccgtagccgc
     4081 gctcgcgcgc cgtgggtacc tcgctcatca attcgcgaat cggcgtgaac aggccggtgt
     4141 aggtggccgg gttgctgcgc ggcgtgcggc cgatcggcga ctggtcgacg ttgatgacct
     4201 tgtcgaagtg ctccaggccc aggatctcct cgtgcgcggc gggctcggta tgggcgcggt
     4261 ggatcttctg cg
//
LOCUS       DAOSLL010000422         4271 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18239_length_4271_cov_3.710626, whole genome shotgun sequence.
ACCESSION   DAOSLL010000422 DAOSLL010000000
VERSION     DAOSLL010000422.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18239_length_4271_cov_3.710626"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="PLB25_16100"
     CDS             <1..844
                     /locus_tag="PLB25_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006156365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MnhB domain-containing protein"
                     /protein_id="HOE43131.1"
                     /translation="LALTQLAVEVVTIVLLLLALYFLPTTTPRESSTGQRWRDGCIAM
                     AAGLGTAALAWAVLTRPVDSIADFFIRQSVPGGGGHNVVNVILVDFRGFDTFGEITVL
                     ALAALGIFALLENLRLPGPTHDALGRAWDKDTHPVIMATFARLLLPLALLVSVFVFLR
                     GHNLPGGGFIAGLITAIALIMQYLANGVDWMHARVPARTHPWIAAGLVMALLTGAGSW
                     VFGRPFLTSAFGHFDLPLVGELELASAMAFDLGVYLVVVGATLLILINLGLAHRASHD
                     EAAT"
     gene            844..1179
                     /locus_tag="PLB25_16105"
     CDS             844..1179
                     /locus_tag="PLB25_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit C"
                     /protein_id="HOE43132.1"
                     /translation="MEALIALTIGTLTGCGVYLVLRARTFAVVLGLTLLSYSVNLFLF
                     ATGRLVVGLPPVIGAAASHADPLPQALVLTAIVIGFAMTAFVIMLSLRTRAELGNDHV
                     DGRDSAGQS"
     gene            1176..2720
                     /locus_tag="PLB25_16110"
     CDS             1176..2720
                     /locus_tag="PLB25_16110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation/H+ antiporter subunit D"
                     /protein_id="HOE43133.1"
                     /translation="MTTVLNWLDHLVIAPVLLPFAAGVLLLLLRDQSMVMQRSLALLA
                     GLLQLGLTIALLLQVADGQMLVYRLGNWPAPWGIVLVADRLAAWMLLLTSLLALCALV
                     YALGGSDRQGRHFHVLFQMQLCGLAGAFLTGDLFNLFVFFEILLIASYGLALHGGGPL
                     RTRAGLHYVVMNLLGSTVFLFAAGLIYSALGSLNLADLAAKAARLPADNLGLARAGGL
                     LLLAVFGLKAALVPLHLWLPAAYANTSAAVAALFAIMTKVGIYAILRTHTLLFGSSGG
                     ELAGLFDPWLLPLAMATLTLATLGVLAAQNLGQMAAYLVVASVGTLLAALALGLPGIA
                     AGLYYLVHSSFSAALLFLLADAIRRRRPLAGDRFTPDADMAQHALWGSLFFFAAIAAA
                     GLPPLSGFVGKFLILRATPQHPWVWAVILLAALASVMALARAGSALFFNTTAALARTG
                     NLAGTPVAPLVPLAAHELLAIGGLIGLILALTAGAGPVLDYVQATAAQLADPALYIQA
                     VLGAGR"
     gene            2717..3205
                     /locus_tag="PLB25_16115"
     CDS             2717..3205
                     /locus_tag="PLB25_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit E"
                     /protein_id="HOE43134.1"
                     /translation="MKRWLPHPLLTPALTVVWLLLNNSATPGQIMLGLLLGWAIPLFT
                     QTFWPEPVRLYRPGALLRFVGVFFHDVLIGNLAVARLVLAGPPALHPMFVQVPLDLRN
                     ELAISLLANIICLTPGTVSARLSEDQRYLLIHALDCQDPQALIATIKTRFEAPLKEIF
                     EC"
     gene            3199..3468
                     /locus_tag="PLB25_16120"
     CDS             3199..3468
                     /locus_tag="PLB25_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015075 - monoatomic ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K+/H+ antiporter subunit F"
                     /protein_id="HOE43135.1"
                     /translation="MLTAALALAFALVAAAFALSFFRLLKGPSAADRILALDTLYINA
                     IALLVLLGIKLASPLYFEAALLIALMGFVGTVALSKFLLRGDIIE"
     gene            3465..3827
                     /locus_tag="PLB25_16125"
     CDS             3465..3827
                     /locus_tag="PLB25_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009871327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter subunit G"
                     /protein_id="HOE43136.1"
                     /translation="MNTSTEVLLSVLVLAGAVFTFIGSLSLVRFKDFYTRLHGPTKAS
                     TLGVGCLLIASSVFFSITGTGLSLHEILVTLFLFITAPISAHLLAKVALHLGLHSIAP
                     LPADVAAPLPSPTPVPHP"
     gene            3938..>4271
                     /locus_tag="PLB25_16130"
     CDS             3938..>4271
                     /locus_tag="PLB25_16130"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HOE43137.1"
                     /translation="MSTIRQADLIESIAAALQYISYYHPADYIAHLARAYEREQSAAA
                     KDAIAQILTNSKMSAEGHRPICQDTGIVNVFLKVGMDLRWDGFTGSLDDAINEGVRRG
                     YNHPDNTLR"
BASE COUNT          610 a         1358 c         1320 g          983 t
ORIGIN      
        1 cctagcgctg acgcagctcg cagtcgaggt ggtgacgatc gtgctgctgc tgctggcact
       61 gtattttctg ccgaccacca caccacgcga atccagcacg ggccaacgct ggcgcgatgg
      121 ctgcatcgct atggcggcgg ggcttggcac ggctgccttg gcctgggcgg tgttgacccg
      181 gccggtggac tcgattgccg acttcttcat tcggcagagc gtgccgggtg gcggcggcca
      241 caacgtggtc aatgtgattc tggtggattt tcgcggcttt gacacctttg gcgaaatcac
      301 cgtgctggcg ctggcggcac tcggcatctt tgccctgctg gaaaacctgc gtctgcccgg
      361 cccgacccac gatgcgctgg ggcgagcctg ggacaaggac acccatccgg tcatcatggc
      421 gacctttgcc cgcctgctgc tgccgctggc gctgctggta tcggtgtttg tctttctgcg
      481 tggccacaac ctgcccggcg gcggctttat tgccggcctg atcaccgcca tcgccctgat
      541 catgcagtac ctggccaatg gggtggactg gatgcacgcc cgcgtgccgg cccgcaccca
      601 cccctggatc gctgccggcc tggtcatggc gctgctcacc ggcgcgggca gttgggtctt
      661 tggccgcccc ttcttgacct cggcgtttgg ccactttgat ttgccgctgg tgggcgaact
      721 ggagctggct tcggccatgg ctttcgatct gggcgtgtac ctggtggtgg tcggcgcgac
      781 gctgctgatc ctgatcaatc ttggcctggc ccaccgcgcc agccatgacg aggcagcgac
      841 ttgatggaag ccctgatcgc actgacgatt ggcacgctga ccggctgcgg ggtctatctg
      901 gtgctgcggg cgcgcacttt tgcggtggtg ctgggcctga ccctgctgtc ctacagtgtc
      961 aacctgtttt tgtttgccac tggtcggctg gtggtaggcc tgccgccggt aattggcgct
     1021 gccgccagcc atgccgaccc gctgccgcag gcgctggtgc tgacggccat cgtcattggc
     1081 tttgccatga cggcttttgt catcatgctc agcttacgca cccgcgccga attgggcaac
     1141 gaccatgtcg atggccgtga cagtgcaggt cagtcatgac gacggtcctg aactggctcg
     1201 atcacctggt cattgccccg gtattgctgc cgtttgcggc aggcgtgctg ctgttgctgc
     1261 tgcgcgacca gtcgatggtc atgcagcgca gcctggcgct gctggccggg ctgttgcagc
     1321 tcggcttgac catcgccttg ctgctgcagg tggccgatgg ccagatgctg gtctatcggc
     1381 tgggtaactg gccggccccc tggggcattg tgctggtggc ggatcgtctg gcggcctgga
     1441 tgctgctgtt gaccagttta ctggccctgt gcgcgctggt ctatgcgctg ggcggcagcg
     1501 acaggcaggg gcggcatttt catgtgctgt tccagatgca gttgtgcggt ctcgcaggtg
     1561 ccttcctgac tggcgatttg ttcaacctgt ttgtgttttt cgagattttg ctgattgcct
     1621 cctacggact ggccttgcac ggcggcgggc cgctgcgcac ccgcgccggg ctgcattatg
     1681 tggtgatgaa cctgctcggc tccaccgtat ttttgtttgc cgctggcctg atttacagtg
     1741 cgctgggcag cctgaatctg gcagatctgg cggccaaggc ggcccgcctg ccagccgaca
     1801 atctggggct ggcccgcgct ggcggcttgc tgctgctggc ggtgtttggc ctcaaggccg
     1861 ccttggtgcc actgcatttg tggctgcccg ctgcctacgc aaacacatct gctgcggtgg
     1921 cggccctgtt cgcgatcatg accaaggtcg gcatttatgc cattttgcgc acccacaccc
     1981 tgctgtttgg cagcagcggc ggggaactgg ctggcctgtt tgatccatgg ctgttaccgc
     2041 tggcaatggc gaccctgaca ctggcgacgc tgggagtgct ggcagcccaa aacctgggcc
     2101 agatggccgc ttacctggtg gtggcctcgg ttggcacctt gcttgccgcc ctggcgctgg
     2161 gcctgccggg cattgcggcc gggctgtatt acctggtcca cagcagcttt tcggcggcct
     2221 tgttgttctt gctggccgac gccattcgcc gccgccgccc gctggccggc gaccgcttta
     2281 cgccagatgc cgacatggcg cagcacgcgc tgtggggtag cctgtttttt ttcgccgcca
     2341 ttgccgctgc cggcttgccg ccgctgtcgg gttttgtcgg caagtttctg atcctgcgag
     2401 caacaccaca acacccttgg gtctgggccg tcattttgct ggcagcgctg gccagtgtca
     2461 tggcgctggc cagggctggc agcgcgctgt ttttcaacac cacagcagcg ctggcccgaa
     2521 ccggcaacct ggcaggcacg cccgttgccc cccttgtgcc ccttgcagcg catgaactgc
     2581 tcgccattgg cggcttgatc gggctgatcc tggcgctcac cgccggggct ggcccggtgc
     2641 ttgactatgt tcaggccacg gcagcacaac tcgctgaccc cgcgctttac atccaggcag
     2701 tattgggggc tggccgatga agcgctggct gccacacccc ctgctgacac cagctctgac
     2761 ggtcgtctgg ctgctgctca acaacagcgc aacaccgggg caaatcatgc tgggcctgct
     2821 gctgggctgg gccattccgt tgtttaccca gaccttctgg ccagagccgg tgcggctcta
     2881 tcgcccgggc gcactgctgc gctttgtcgg tgtgtttttt catgacgtgc tgattggcaa
     2941 cctggcggtg gcgcgattgg tgctggccgg gccaccggcg ctgcacccga tgtttgtgca
     3001 ggtgccactg gacttgcgca acgaactggc catcagcctg ctggccaata tcatctgcct
     3061 gacacctggc acggtgtcgg cgcgcttgtc tgaagaccag cgctatttgc tgatccatgc
     3121 gcttgactgc caggacccgc aagccctgat tgccaccatc aaaacgcgtt tcgaggcccc
     3181 gctgaaggag atttttgaat gctgactgcc gccctcgctt tggccttcgc cttggtcgcc
     3241 gccgcttttg cactgagttt tttcaggctg ctcaaaggtc cgagtgcggc cgaccggatt
     3301 ctggcgcttg atacgctcta catcaatgcc atcgccctgc tggtgctgct gggcattaag
     3361 ttggccagtc cgctgtattt tgaggcagca ctgctgattg ccttgatggg ctttgtcggc
     3421 accgtggcat tgtcgaaatt cctgctgcgc ggagacatca tcgaatgaac accagcaccg
     3481 aagtcctgct gtctgtcctg gtgctggccg gtgcggtgtt caccttcatt ggctcgctca
     3541 gtctggtgcg gttcaaggat ttttacaccc ggctgcacgg ccccaccaag gccagcacgc
     3601 tcggcgtggg ctgcctgttg atcgcctcct cggttttttt cagcatcacc ggcaccggcc
     3661 tgagcctgca tgagatattg gtgacgctgt ttctgttcat cacggcaccg atatcggcgc
     3721 atctgctggc caaggtggca ctgcacctgg gcctgcattc gattgcgccg ctacccgcag
     3781 acgttgctgc gccactgcca tccccgacac ccgtgcccca cccttaagca cctggccgac
     3841 gcgtcaggct agcgttgggg cgggttggtt aaacttcgtt catgaaccga tttttcgcgc
     3901 ctaccccctc ttcgcaagcc ttcaaaggaa cccctcaatg agcaccattc gccaagccga
     3961 ccttatcgaa tccattgccg ccgccctgca atacatcagc tattaccacc ctgctgacta
     4021 cattgcgcac ttggcgcgtg cctatgagcg cgagcaaagt gctgccgcca aagatgccat
     4081 tgcgcaaatt ttgaccaaca gcaagatgtc ggcagaaggc catcggccga tttgccagga
     4141 caccggcatc gtcaacgtgt ttctcaaagt tggcatggac ctgcgctggg acggcttcac
     4201 cggttcactt gacgatgcca tcaacgaagg tgtgcgccgt ggctacaacc accccgacaa
     4261 cacgctgcgc g
//
LOCUS       DAOSLL010000423         4268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18263_length_4268_cov_3.680513, whole genome shotgun sequence.
ACCESSION   DAOSLL010000423 DAOSLL010000000
VERSION     DAOSLL010000423.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18263_length_4268_cov_3.680513"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            191..592
                     /locus_tag="PLB25_16135"
     CDS             191..592
                     /locus_tag="PLB25_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012436152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43138.1"
                     /translation="MKSTFFKMVTILIAAVVMAGLGACGSDADVASRNMSKAADQFEV
                     TRRIVFYNGITADYMLTIQGQCSLGNQDPHRQLSITCKTGPSSYKKHFLGLSDNVTYF
                     VEQLDAAPASTYQYRVVFKPLAMVPDIEMPK"
     gene            616..807
                     /locus_tag="PLB25_16140"
     CDS             616..807
                     /locus_tag="PLB25_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43139.1"
                     /translation="MTFLLIWTFVGAQSLVAGHHQTAQYDWRQLASFSSGAACHQAAR
                     LLNISDNKHRCVNADGVLQ"
     gene            804..1058
                     /locus_tag="PLB25_16145"
     CDS             804..1058
                     /locus_tag="PLB25_16145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43140.1"
                     /translation="MTSAAIFSDISALALAAGAVNLRRVPVAADAGRICADLLSRVQS
                     RTPRPNPPEPDDQGVLASCTKGPRKPAGGSVASFAMNPVL"
     assembly_gap    1116..1125
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1126..1618
                     /locus_tag="PLB25_16150"
     CDS             <1126..1618
                     /locus_tag="PLB25_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43141.1"
                     /translation="VTIAGYAFIRWSERRDAKAAVREIQAIKQICETAHARAFSVAAS
                     LPAAQLATLHQAVSAAVAEGDDCAEFRQRLPTALFPWPQGPLAADFECRSAQVPPAQP
                     CIEQAITDIGLALCASHGTIATDLPDAVVTDTSWRIDHTHEMAQLAVLQAALRTVNSG
                     SAP"
     gene            complement(1596..1796)
                     /locus_tag="PLB25_16155"
     CDS             complement(1596..1796)
                     /locus_tag="PLB25_16155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011352098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HOE43142.1"
                     /translation="MEMIRVSSSAISAVGYEPSTQQMTIKFTQGQSYTFCRVPAAVFD
                     GLMRSGSKGSYYDSHIKGRYRC"
     gene            complement(1896..2672)
                     /locus_tag="PLB25_16160"
     CDS             complement(1896..2672)
                     /locus_tag="PLB25_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43143.1"
                     /translation="MNLNPKIILGAIALLVALSIGGNIIHNIEANSAQAEAEAARAAK
                     AKLVQEKRDALMTEFHGNRSALISESQQAISQGDPATAQKALSKFASLDDPDVKHLLQ
                     VAGDRLQAAQTIKKLSDELTNKPDKVRALAIYTELARLEPSNPLWRALIEENTPIVAA
                     IKARLAAAEKVAARKDAVKRLFSVSDGSVYAVEVGIKARLKDPDSYKHVETRASDPGV
                     GDVTVFTQYRARNSFNAVVPGLATAVVSPSGELVSLELHK"
     gene            complement(2704..3147)
                     /locus_tag="PLB25_16165"
     CDS             complement(2704..3147)
                     /locus_tag="PLB25_16165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43144.1"
                     /translation="MKKTCIKCNHVNLSATGDDTEACPACGVIYAKAQPPLARPAVTP
                     SVKRAQRNEPFIDRLRDESLYPTFRQVVTIFHVLGQILAVLFFLGGLVSGYKMGNTGM
                     MVGSTIGAIFMSVFFRVAKEASLMLADMSDATVRLAQRADADSSF"
     gene            complement(3173..3643)
                     /locus_tag="PLB25_16170"
     CDS             complement(3173..3643)
                     /locus_tag="PLB25_16170"
                     /inference="COORDINATES: protein motif:HMM:NF025005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HOE43145.1"
                     /translation="MTKSVHQTINASVHGNVAEGNITIQQTIHHHGLLSVGCGAPTPT
                     RSRCARMWCMLLVVTLLLCAVALAVVLAAQPAAPAPSSGGWPAPTVPLRPVTSSAIVA
                     MGYDPITQQLYIVFTSSPKPYTYCAVPAPVFDAFMAATSKGAYFHQAVRGRFGC"
     gene            complement(3671..>4268)
                     /locus_tag="PLB25_16175"
     CDS             complement(3671..>4268)
                     /locus_tag="PLB25_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43146.1"
                     /translation="ERVRTKQKDLAAILEVPLQRVKRLTGGEVQKLTPDETRRLVQTL
                     NLSAHWLATGEGPMFNTQGGQKLGETLTQLRLTTARLTEMEVDEPVASAVVSIVLGVA
                     DKDSHSLREGLIQAANAMQTPDERALLDTYRRCTPQARANLIQTAALLSAGMAAPDCA
                     PKRITKAPPRRVHQVVTAQVGGHVVSGNLNINSHNTKK"
BASE COUNT          904 a         1249 c         1172 g          933 t
ORIGIN      
        1 tcgtccaggg cgatgcctac cacgcctgcg gcggatagca ctaagactgc gatcaaggaa
       61 cggttggggt ctgccatggc cccaactgtc gcgcgcgcgc cagggtttgt aattggaacc
      121 ggggcacatt acggcgcggt gcggcgaagc ggaaactcaa agcctttcag caaatcaacc
      181 cgacaaattc atgaaatcta cttttttcaa aatggtgacg atacttattg cggcagtcgt
      241 catggcaggt cttggtgcgt gcggaagcga tgccgatgta gcgtcaagaa acatgtcaaa
      301 agccgccgac caatttgagg taacccggcg aatcgtcttc tacaacggca tcaccgcaga
      361 ctacatgctg accatacagg ggcagtgttc actgggcaat caagatccgc accggcagct
      421 atccatcacc tgcaaaaccg gccccagctc ctacaaaaag cactttcttg gcctgtctga
      481 caatgtgacg tactttgttg agcaactgga cgcggcaccg gccagcacgt accaataccg
      541 tgtggtcttc aagccgcttg ccatggtgcc ggacattgag atgcccaagt agagaaaagt
      601 cagcagcttt tcaaaatgac attcctgtta atctggacct ttgtgggggc gcagtcactg
      661 gtcgccggcc accaccaaac ggcacaatac gactggcgcc aacttgccag cttcagcagc
      721 ggtgccgcct gtcatcaagc ggcgcggttg ctcaatatca gtgacaacaa gcaccgctgc
      781 gtaaacgctg atggggtgct gcaatgactt ctgccgccat cttctccgac atctctgcac
      841 ttgcgctggc tgccggtgcc gtcaatctgc gccgggtacc ggtggcggca gatgccgggc
      901 gaatttgcgc cgatctgctg tcacgcgtgc aaagccgtac accgcgccca aacccgccag
      961 agcctgatga tcagggcgtg ctggcaagct gcaccaaagg gccgcgcaag ccagcaggcg
     1021 gttcagttgc ttcttttgcg atgaacccag ttctttaaac gaatcaaagg taactcgaat
     1081 ggatctccta ctatattttt gtggcgttgt cggcgnnnnn nnnnncgtaa cgattgcggg
     1141 gtatgccttc atccgctggt cagagcggcg cgatgccaaa gctgctgttc gggagattca
     1201 agccatcaaa caaatctgtg aaaccgcaca cgccagggcc ttcagtgttg ccgcaagctt
     1261 gccagcagcg caattggcca cgctgcacca ggcggtcagt gccgcagttg cagaagggga
     1321 tgactgcgct gaatttcggc agcgattgcc gacagcgctt tttccttggc cacaggggcc
     1381 attagccgct gactttgaat gccgtagcgc acaagtgccc cccgcgcaac cctgcattga
     1441 gcaggccatc acagacattg gcctggccct gtgcgccagc catggcacga ttgccacaga
     1501 cctgccagac gcggtggtca cagacaccag ttggcgcatc gaccacaccc atgaaatggc
     1561 gcaactggcc gtgctacagg ccgcactgcg cacggttaac agcggtagcg ccccttgatg
     1621 tgactgtcgt aatagctgcc cttggagcct gatcgcatca agccatcaaa cacggcggct
     1681 ggaacccggc agaacgtgta gctttgcccc tgggtaaatt tgatcgtcat ctgttgtgtg
     1741 ctgggctcat agcccaccgc actgatggcg ctcgaactga ctctgatcat ttccatattc
     1801 actcctggtt aaaaaagctt caagccaaat cggccgacag cccggatgcg gcttgcgcgg
     1861 ggtgctataa aaattaccag cctgccttca cgatgtcact tatgcaactc caatgaaacc
     1921 aactcacctg acggcgaaac aacagcggtt gccaagcccg gtaccacagc gttgaaagaa
     1981 tttctagccc ggtactgcgt aaataccgtc acatcgccca cacccggatc actggctctt
     2041 gtctcaacgt gtttatagga atctgggtct ttcagtcgag ctttgatgcc cacttcaacc
     2101 gcgtaaacag atccatcaga aacgctgaac aaccgtttaa ccgcatcctt tcgcgccgct
     2161 accttttctg ctgctgccag ccgtgctttg atggctgcga cgatgggtgt gttttcctca
     2221 atcagagcgc gccaaagcgg attggatggc tccaaacgag ccagctccgt atagatcgcc
     2281 agcgcccgca ccttgtctgg cttgttggtg agctcatctg acaatttttt aatcgtctgc
     2341 gccgcttgca acctatcacc agccacctgc aaaaggtgtt ttacatccgg gtcatcgagt
     2401 gatgcaaatt ttgaaagcgc cttctgcgct gttgcgggat caccttgact aattgcctgc
     2461 tgagattctg agatgagcgc agacctatta ccgtgaaatt cagtcatcaa ggcatcacgc
     2521 ttttcctgaa ccagcttcgc cttggcagcc cgggctgcct ctgcttccgc ctgcgcgctg
     2581 ttcgcctcaa tgttgtgaat gatgttgcca ccaatagaca acgcgaccag caacgcaata
     2641 gcacccaaaa tgatctttgg atttaaattc atggctgacc tccatttttc gcgtggtgat
     2701 gctttaaaaa ctgctgtcag catccgcccg ttgcgccaac cgcaccgttg catcgctcat
     2761 atctgccagc atcagcgatg cctcttttgc cacccgaaaa aagaccgaca taaaaatggc
     2821 accaatggtg gatccaacca tcatgccggt gttgcccatt ttgtaaccac taaccaagcc
     2881 gcccagaaaa aacagcacag ccagtatttg gcccaacaca tggaagatcg tcacgacctg
     2941 gcgaaatgtt gggtagagac tttcatcgcg caggcggtcg atgaacggct cattgcgctg
     3001 cgcgcgcttc acactgggtg tcactgccgg gcgcgccaaa gggggctgtg cttttgcata
     3061 aatgacaccg cacgccgggc aggcttcggt gtcatcaccc gtagcggaca aattgacgtg
     3121 gttgcacttg atacaggttt ttttcatgac tgactccttg gtaaagacaa actcagcacc
     3181 caaaccgccc gcgcaccgcc tggtggaaat acgcgccttt tgaagtggct gccatgaagg
     3241 catcaaacac aggcgcggga acggcgcaat aggtgtaggg tttagggctg ctggtgaaga
     3301 cgatgtagag ctgctgcgtg atcgggtcgt agcccatggc cacaatggcg ctggatgtca
     3361 ctggccgcag tggcaccgtg ggcgcaggcc atccgccaga gcttggcgcg ggggccgcgg
     3421 gctgcgctgc caggacaacg gccagcgcta cggcacacag caacagcgtc accaccagca
     3481 acatgcacca catccgggca caccggctgc gggtgggcgt cggcgcacca cagcccacag
     3541 acagcaagcc gtggtgatgg atcgtctgct ggatggtgat attgccttcc gccacattgc
     3601 cgtgcaccga ggcattgatg gtttggtgga ctgacttggt cactgtctgt tgtctccccg
     3661 ggccactgtt ttattttttg gtgttgtggc tgttgatgtt gaggttgccg cttaccacat
     3721 ggccgcctac ctgggcggtg acgacctggt gcactcgcct tggcggcgcc ttggtgatgc
     3781 gcttgggggc gcagtcgggg gcggccatgc ctgctgatag cagcgccgct gtctgaatca
     3841 gattggcgcg ggcctgtggt gtgcagcgac ggtaggtgtc gagcaatgca cgctcatcgg
     3901 gcgtttgcat ggcattggca gcctgtatca gaccttccct gagcgaatgt gagtctttgt
     3961 cggccacgcc aaggacgatg ctgacgacgg ctgatgccac aggctcatcg acctccattt
     4021 cggtcaggcg cgctgtcgtc agccggagct gcgtcagtgt ttcgcccagc ttctggccac
     4081 cctgggtatt gaacatgggg ccttccccgg tcgccagcca gtgcgcactg aggttcagcg
     4141 tttggaccag gcgccgggtt tcgtccggcg tgagcttctg aacctcaccg cctgtaagtc
     4201 tttttacgcg ctgaagtggc acctcaagaa ttgctgccaa gtccttctgc ttagtgcgca
     4261 ctctttca
//
LOCUS       DAOSLL010000424         4268 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18265_length_4268_cov_3.365773, whole genome shotgun sequence.
ACCESSION   DAOSLL010000424 DAOSLL010000000
VERSION     DAOSLL010000424.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18265_length_4268_cov_3.365773"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="PLB25_16180"
     CDS             complement(<1..392)
                     /locus_tag="PLB25_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43147.1"
                     /translation="MRQRQHREFIGAISFHFEELNQAFGRGKFRAINDRLGIFKVSTN
                     WNAGLNSPCRSAHTKAYWFSEAVENARRSYLCSITPSGTKLRMMSGSVLKSLPKAVAS
                     KDVSGVTTTAWSHATDLNRVSVDLQLLL"
     gene            complement(668..2380)
                     /locus_tag="PLB25_16185"
     CDS             complement(668..2380)
                     /locus_tag="PLB25_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43148.1"
                     /translation="MHLLAIEATHSIGAPIDKHISPDTCILTDAVTGAPVDHQDHSDD
                     PKASAVATVNPTVMVSCATDSVERRVEQTEFKAETRPSICVRPGHLQEMIGEAAHHLA
                     MTGNFFQYEGATVAIHIDAASGESGIQALQPMDMAYAMDGVMVWQKEDKRSKSWSKID
                     PPERICKMLTTITKHGQLPVLNGLTRQPFLRPDGSLCTNSGYDSATGLYGVFDANDFD
                     VPEMPSRRQAEEALTLLQGLLNEFSFASESDQSAALAAMLTAAIRSSFAAALMFHVRA
                     PQIASGKTYLCEIITAFASPQPGSPIGFPNSNDECSKLLVAELLCSPAVIEFDNLTSD
                     IKPYKSLCTALTSGRMKGRILGVSDMAKVGTRTLFLSSGNNVGPVADMARRCVTIHLD
                     PKCEVPATRTYKNPNLIAEVLRARGRYVSAALTIVRAWITAGRPTTECKPVNSFGEWT
                     ALCRQPLLWLNQPDPATSVFNGMAEDPENEITGQFLGSWFALFGDKAMLVRDAISHSG
                     HLSQDAEEFKEVMYEVAGERGKINSRKAGHWMKRHEGRVVNGLRLTRCPKTRNVESWR
                     VESV"
     gene            complement(2498..2713)
                     /locus_tag="PLB25_16190"
     CDS             complement(2498..2713)
                     /locus_tag="PLB25_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43149.1"
                     /translation="MELETRFAVGTEVAAFHCNRRPQTLRAWACRENGPMRPINVNGR
                     LAWPVSEIRRVLGCPTPSIGKKRPEGS"
     gene            complement(3007..>3283)
                     /locus_tag="PLB25_16195"
     CDS             complement(3007..>3283)
                     /locus_tag="PLB25_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43150.1"
                     /translation="QGGFDIAYGSDARDRQFLHVKAQASNINLLFRDPASDCTSAWNP
                     LRAEVVVAMGTQPVAPKKHETPCNLEVSEFAILKKKLGLDGSHPASW"
     assembly_gap    3284..3293
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3294..3613)
                     /locus_tag="PLB25_16200"
     CDS             complement(<3294..3613)
                     /locus_tag="PLB25_16200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43151.1"
                     /translation="MKKTNRENLPVWSAVYGVKPNTVIDSLENLMTSVALNAGWFELL
                     FGLDTSQRGFLYVQTLKDSFWLNATPRANSKARKSRCSFSWVYAIDGLPVEFYKNGYE
                     PTVLT"
     gene            complement(3811..3972)
                     /locus_tag="PLB25_16205"
     CDS             complement(3811..3972)
                     /locus_tag="PLB25_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43152.1"
                     /translation="MKTSKQSDSNPLDLGTLPFNTTTRAAALLMDGAVSSTPMYDMAK
                     WHQAHHAIE"
     gene            complement(4065..>4268)
                     /locus_tag="PLB25_16210"
     CDS             complement(4065..>4268)
                     /locus_tag="PLB25_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43153.1"
                     /translation="LDQELYYVAYAIAMAGRWGAARLLREIFKEHRDLTVARSTAIRI
                     IERVERTRKTDLEREVQKRKILY"
BASE COUNT          974 a         1119 c         1084 g         1081 t
ORIGIN      
        1 tgtagcaaca actgcaagtc aacgctcacc cggttaaggt cagttgcgtg actccaggcg
       61 gtcgtcgtaa ccccagaaac gtccttggat gctaccgctt tgggcaaaga cttcaataca
      121 ctcccgctca tcatgcgtag cttggtcccc gatggcgtaa tactgcacaa ataggaccgc
      181 cttgcatttt ctacagcttc tgaaaaccaa taggctttcg tatgtgcaga gcgacaaggc
      241 gaattcaagc cagcattcca gttggtcgat accttgaaaa tcccaagccg atcattgatg
      301 gccctgaact tgccccgacc aaacgcttga ttgagttcct caaaatgaaa ggaaatagcg
      361 ccaatgaatt cgcggtgttg gcgctgcctc atccgaatca ctgagttcga ggttgatggc
      421 tccaacgatg acggcggcta ttgaaatgca aaatttgtga tgtgcggaaa atagtgcctg
      481 cctggctcaa actggcgcac ccaagccatt tcgatggtct ggcttccgtc actgaccacc
      541 tgaaaagatg gtgcccgtga cgatcaatct catttgacaa atatcactgg cagtctttgg
      601 atcacacgca tttcatggca cttttgctta atgcgaaatc aggaaccgac taaaccgact
      661 aaaccgacta aaccgactcc acccgccagc tttcaacgtt gcgagtcttg gggcacctcg
      721 tcagacgcag accgttgaca acccgacctt cgtgccgctt catccagtga ccggccttgc
      781 ggctgttgat ctttcccctc tcaccggcta cctcatacat gacctctttg aattcttctg
      841 cgtcctgact caggtgaccc gaatgcgaaa tagcgtcacg caccaacatc gccttgtcac
      901 caaaaagcgc gaaccagctt ccgagaaact gtcctgtaat ttcattctct ggatcttccg
      961 ccatgccgtt aaacaccgat gtcgctggat cgggttggtt cagccacagc aatggttgac
     1021 ggcaaagtgc tgtccattca ccgaaactgt ttaccggctt gcactctgta gtcggccgtc
     1081 cagcggtgat ccacgcgcga acgatggtca gagccgcact gacgtagcga cccctggcac
     1141 gtaacacctc ggcgatcaag ttgggattct tgtaggtgcg cgtcgcgggc acctcgcatt
     1201 ttgggtccag atgaatggtc acacagcggc gggccatgtc agcgacgggg ccgacgttgt
     1261 tgccactgct caggaaaagt gtccgcgtgc caaccttggc catatctgaa actcccaaaa
     1321 tacgaccctt catacgtccg ctggtgagcg ccgtgcagag acttttgtac ggttttatgt
     1381 cgcttgtcaa attgtcgaac tcaatgaccg caggtgagca caacagctcc gccaccaaca
     1441 gtttgctgca ttcgtcattg ctgttgggaa atccaatggg gctgcccggt tgtggtgaag
     1501 cgaacgcagt gatgatttca cacagatagg ttttaccgga cgcgatctgc ggtgcccgga
     1561 catgaaacat aagcgccgcg gcaaagctgc tacgtatcgc tgcggtcaac atggccgcca
     1621 atgccgcgct ttggtcactt tcagatgcaa atgaaaactc attcagtaaa ccctgcaata
     1681 gcgtcaacgc ctcttcagcc tgcctgcggg atggcatttc aggcacgtca aaatcgttgg
     1741 catcaaaaac gccgtagagg cccgttgcag agtcgtaccc gctgtttgtg catagcgaac
     1801 cgtccgggcg cagaaatggc tgacgggtca atccgttcag gacaggcaat tgcccatgtt
     1861 tggtgatggt ggtcagcatc ttgcaaattc gctcaggcgg gtcaattttt gaccaggatt
     1921 tgctgcgctt gtcttctttc tgccagacca tcacaccatc catggcataa gccatatcca
     1981 tcggttgcag ggcttggatg ccagactcac cagatgccgc gtcgatatgt atcgctacgg
     2041 ttgccccttc atattggaaa aaattgcctg tcatggccag gtgatgcgcg gcttcgccga
     2101 tcatttcctg aaggtggcca ggccttacgc aaatgctcgg acgtgtttcg gccttgaact
     2161 cagtctgctc gacacggcgt tcgacggaat ctgtggcgca agagaccatc acggttgggt
     2221 tgacggtggc aacggcactg gctttgggat catctgaatg atcttgatga tcgactggcg
     2281 ctccagtaac agcatcagtc aaaatgcatg tatcaggcga aatgtgcttg tcgattggtg
     2341 ccccgataga gtgggttgct tcaatggcta ataaatgcat ttggtttttc tcgcttgtat
     2401 tttgttttga cctgccgcca ggacatgtcc tgtaatgggt tggaatgttg actcgcttcc
     2461 caaagagcac ctgagtgctg attggggtgc taccacttca tgagccctcg gggcgctttt
     2521 ttccaatgga cggggttggg caaccaagta cacggcggat ttcactcacg ggccatgcta
     2581 gccgtccgtt tacgtttatc gggcgcattg gcccgttttc gcggcaagcc catgcgcgca
     2641 atgtttgtgg gcggcggttg caatgaaagg ccgcgacctc agtacccacg gcaaagcgtg
     2701 tttccaattc caggggaaca aaagcagtgg gcgcttttcc gacctgcgga gaacgactac
     2761 caggtccttg agtgactgga ccgctgccct tttcggatgt gttagcgcaa gtcgagataa
     2821 ccttcgcggt gatcagcgaa cgcatatcct caccaaattt ggaagaagtc tcaggtgtct
     2881 cagcattcaa caggttttgt ttcatttgat tcattctttg atttgggttg gaggccaagc
     2941 ctgacctttg caatcagatc cgccaggctt ggcttgaggg tcagggtgaa gttctgttaa
     3001 gtacggttac caagatgccg gatgcgaccc atccaacccg agcttctttt tgagtatggc
     3061 gaattcagaa acctctaagt tgcagggtgt ttcatgtttc tttggagcga cgggttgagt
     3121 acccattgca accaccactt cagcccgcag ggggttccaa gctgaagtgc aatcagacgc
     3181 tggatcgcga aatagcaggt tgatgttgct tgcctgcgcc ttaacgtgca aaaactgacg
     3241 gtcacgtgca tctgacccat aagcaatatc gaatccgccc tgtnnnnnnn nnngtcagca
     3301 cagtcggttc gtatccgttt ttgtagaact caaccggcag gccatcgatt gcatacaccc
     3361 aactgaagct gcagcgtgat ttccgtgctt tagagtttgc ccggggtgtg gcgtttaacc
     3421 aaaagctgtc tttgagcgtt tggacataca aaaatccgcg ctgtgatgta tccaagccaa
     3481 aaagcaactc gaaccatccc gcattgagtg cgacactggt catcaagttt tcaagtgagt
     3541 cgatcaccgt atttggcttg acaccataca ctgcagacca gacaggaagg ttttcacgat
     3601 tagttttttt catggttttc tcaaatgttt tggacatgcc gaaatcggcg atggagtacc
     3661 tgatgcaggt gacgacaaac gggttgcagt aacaaaaggg cgtggtgttc ccatccgcct
     3721 tgcttatcgc aaagcagtga tgaactgaac tggtgtgatt gcccggtctg ggtttactca
     3781 acccaatcgg tcgattgacc gactagaccg ctattcgatt gcgtgatgtg cctgatgcca
     3841 cttggccatg tcatacatgg gcgttgatga cacggcgcca tccattagga gtgcggccgc
     3901 tctggtcgtt gtgttgaacg gcaaggtgcc tagatcaaga gggttgctat cagactgttt
     3961 actggttttc atcaaattct cctggttaaa agtgcctcag aagaacttct gctgccaaaa
     4021 agaattggaa tacctgtaaa ttggctcggg gaagctttca aaaatcaata taaaattttt
     4081 ctcttctgga cttctcgttc taggtcggtt ttccttgtcc tttcaacgcg ctcaatgatc
     4141 ctgatcgcag ttgaacgagc cactgtcaga tctcgatgtt ctttaaaaat ctctctcagt
     4201 agtcttgccg ctccccaccg tcctgccatg gcaatggcat atgccacata atacaattcc
     4261 tggtccaa
//
LOCUS       DAOSLL010000425         4264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18289_length_4264_cov_4.825612, whole genome shotgun sequence.
ACCESSION   DAOSLL010000425 DAOSLL010000000
VERSION     DAOSLL010000425.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4264
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18289_length_4264_cov_4.825612"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            558..1103
                     /locus_tag="PLB25_16215"
     CDS             558..1103
                     /locus_tag="PLB25_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HOE43154.1"
                     /translation="MKRIIFATVFASVLFGAQAETYTDSARVRSAEAQYENVNVPRNE
                     CSRHWVEERGGRSAYEPHVGQERQYGGAIVGGLAGGVIGHQIGAGTGKDAATALGVVL
                     GAIAGDRIENEGRRPPYERPARTFSRREVESCRTVYDTQTRLTGYRVAYEYRGQTYTT
                     FMRSNPGNSLPVRVSVDPIMQ"
     gene            1327..1563
                     /locus_tag="PLB25_16220"
     CDS             1327..1563
                     /locus_tag="PLB25_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HOE43155.1"
                     /translation="MKFLTALTTALLMTLTAPVLADVSREDAAATVQKISGGRVLAVD
                     KTDRDGRSVWRVKVLMPQGEVKVILVDIASGRTL"
     gene            1586..2257
                     /locus_tag="PLB25_16225"
     CDS             1586..2257
                     /locus_tag="PLB25_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE43156.1"
                     /translation="MRLLLIEDDAVLRLGVKRQLEADGYRVDTAVDGEDGLFQAREYP
                     VDLAIVDLGLPKLNGLAVVQTLRAEGRALPILILTARGSWQDKVKGLETGADDYLVKP
                     FDYPELAARVKALLRRALKATSNVLRLGALEIDFSAQAARLDGEVLDLTTFEYRVLEY
                     LVRERARVVSKQELSDYLYPHDEDRDSNVLEVLVGRLRRKLDPESKTAPIETLRGRGY
                     RFVLQ"
     gene            2308..3615
                     /locus_tag="PLB25_16230"
     CDS             2308..3615
                     /locus_tag="PLB25_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE43157.1"
                     /translation="MLTFLAVAGWAVRQAYADGIRSQHFARLQTTIYLLMAGAELDSA
                     GGLVMPKSLAEPRLSLPASGLYASINNLNKGQSWQSPSTLGTDPPFQRSQVTGQWQFD
                     AVNATPAQGDDHVASGSAFLAAAYAVKWAVNDQVASLVFSVLEDKVEFDRELATFERT
                     LWSWLVATGLLMLITQTLVLRWGLMPLGQMALEIRQIETGAQTRIEASYPVELAGLGD
                     NLNLLIDQQRARQTRYKDALDDLAHSLKTPLAAMRASLAQPDELAAAVSQQVSRMDDI
                     VRHQLGRAGASGVTRFAPHLTVAPLLNRICDTMAKVYVDKELSFVIDCPPELTWRVDE
                     GDAFEILGNLLDNAAKWAVHEVCLRIWLDEDAMCMRVTDDGPGFADPQARLARGVRMD
                     ESVPGHGIGLTVVNDLVASYLGELKISNRLTGGAQIEIVLPKT"
     gene            complement(3974..>4264)
                     /locus_tag="PLB25_16235"
     CDS             complement(3974..>4264)
                     /locus_tag="PLB25_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43158.1"
                     /translation="FGRFPQGDWRFRSRTIYTAATTYYRLHNFARRNTVRHWQHRGPY
                     GDYNHNGIANIYEAGRVRSHLHHASLHGSYRDLDRDGIRNRFDHRPGNPYRR"
BASE COUNT          885 a         1111 c         1292 g          976 t
ORIGIN      
        1 atctctaaaa ccaaaaattg gcttgcgatt cgattggttg agaaggcttc gctggtttaa
       61 gttcgagcgt tgaccattgg gtcagaaaga ccaaagcggc tcacaatgtg agccgctttc
      121 aattcaggcg tgtgttttga tgatccgcca gttctttatg aagatgtgcg agtgcgtcca
      181 ccccgaggat tcatcgatat cacgtcagtg aactgtcctc gagtcccacc gagcgtcact
      241 gctacagtgt atttttcaaa ctggtttgcg caggcaatga cgaagagcca aattctgatg
      301 cctatggtca cccaaacttc cccatggtag tggttaagaa tgttcgattt cagaagaatt
      361 gtcagcggct cggttttgga tgctgttctt gaaatccgac ttgttggcct gccaaatctc
      421 agcgccactg gtgtttactc tgttgtttca gccgaagggg tcgccggtct ggctgcaacg
      481 acaatattga ttctgttcag cttgggttca tgcacggacg cgcaaactgt cacctgtact
      541 aacccaagga gccttttatg aaacgaatca tcttcgccac tgtgtttgcc agtgtattgt
      601 ttggtgccca ggcagaaacc tacacagaca gcgcccgtgt tcgcagcgct gaagcgcaat
      661 acgaaaacgt caacgttcca cgcaatgagt gcagcaggca ctgggtcgaa gaaagaggtg
      721 gccgcagcgc ctatgaacct catgtcggcc aggaacgcca gtatggtggc gcgattgttg
      781 gcggcttggc gggtggtgtc atcggccacc aaatcggtgc tggcaccggc aaagacgctg
      841 caaccgctct gggtgtggtg ttgggcgcga ttgccggtga ccgcatcgag aacgagggtc
      901 gccgtccccc gtatgaacgc cctgcgcgca cgttcagtcg gcgagaagtc gagagttgcc
      961 gcaccgtgta tgacacgcaa acacgcctta caggttaccg agttgcttat gaatacaggg
     1021 gccagaccta caccacgttc atgcgatcca acccgggcaa cagtttgcca gtgcgcgtgt
     1081 cggtcgaccc gatcatgcag taaggatgtg gtcagaaata gctttcccaa tttgcgtcgg
     1141 cggatttggg cgcactgcgt tgttgcaaga cgcttgttta gccagctaaa cggagcgtct
     1201 tgtgcctagc cgcgcatccc aactccttgg tgcaaaacgg ggagttggtc ccgacaacat
     1261 cctacgcgcc aacctgtcgg ccagagtacc attgtctcta gccgtgacgt aatacctgag
     1321 cttgccatga aattcttgac tgcactgacc accgccttgc tgatgaccct gacagcgccc
     1381 gtcttggcag atgtcagccg cgaagatgcg gcagcaacag tgcagaagat cagtggtggg
     1441 cgcgtgttgg cggtggataa aaccgaccgc gatggtcgtt cagtctggcg cgtgaaagtg
     1501 ctcatgccgc agggtgaagt caaagtgatc ctggttgata tcgctagtgg gcgcacgctc
     1561 tgattggcct gcaaggtcat cgatcatgcg cttgttgctg attgaagatg atgccgtgtt
     1621 gcggcttggt gttaaacgcc agcttgaggc cgatggttac cgggtcgaca cggcagtaga
     1681 tggggaagac ggtctgtttc aggcccgcga gtatccggta gatcttgcca tcgtggacct
     1741 gggacttccc aagctcaatg gactggctgt ggtccagacc ctgcgcgccg aaggccgggc
     1801 tctgccaatc ttgatactga cggcgcgtgg cagttggcag gacaaagtca aaggtctgga
     1861 gaccggtgcc gacgactacc tggtaaagcc cttcgactat ccggaactgg ctgctcgcgt
     1921 caaggcgctg ctgcgacgtg cactgaaggc gacatcgaat gtgcttaggc tgggcgcact
     1981 tgagatcgat ttttcggccc aagccgcgag actcgacggt gaggtcctgg atctgaccac
     2041 ctttgagtac cgggtgctcg aatacttggt gcgcgaacgt gccagggtgg tgagcaaaca
     2101 ggagttgtct gactacctgt acccgcacga tgaggaccga gacagcaacg tgctggaggt
     2161 tttggtcggg cgacttcggc gcaaacttga ccctgagagc aaaactgccc caattgaaac
     2221 gctgcgcggt cgaggctacc gcttcgtact gcagtgaacg ctccgccgtc catacgttct
     2281 cgtttggcat ggggcgcttt cctggtcttg ctcacgtttt tggcggttgc tggctgggcg
     2341 gttcgacagg cttacgctga cggcatccgg tcgcagcact ttgcccgatt gcaaaccaca
     2401 atctatttgt tgatggccgg tgctgaactg gactcagcgg gtgggttggt catgccgaag
     2461 tcgctggctg aacctcgttt gtccttgcca gcatcgggac tgtatgccag catcaataac
     2521 ctgaacaaag gtcaatcgtg gcagtcgccg tcaacgctgg ggactgatcc accgtttcag
     2581 cgaagtcagg tcactggtca atggcagttt gacgcggtca atgccacacc ggcgcaaggc
     2641 gatgaccatg tcgcttctgg cagcgcgttt ctggccgcag cctacgcagt taaatgggct
     2701 gtgaacgacc aggtggccag tctggttttc tcggttctgg aggacaaggt cgagtttgat
     2761 cgggagttgg cgaccttcga gcgcaccctc tggagctggc tggtagcgac gggcctcctc
     2821 atgctgatca cgcaaacctt ggtgctgcgt tggggattga tgccacttgg gcagatggct
     2881 ttggaaatcc ggcagatcga gaccggcgct caaacccgga ttgaggcaag ctatccagtt
     2941 gaacttgccg ggcttggcga caatcttaat ctgctcattg accagcagcg tgcccgccaa
     3001 actcggtaca aagacgcgct tgacgacctt gcccatagtc tgaagacccc gctggcggcc
     3061 atgcgcgctt cattggctca gcccgatgaa ttggccgctg ctgtgtcgca acaggtcagt
     3121 cgcatggacg acatcgtgcg tcaccaattg gggcgcgccg gtgccagcgg agtcacccgg
     3181 tttgcgcccc atttgacagt tgcacctttg ctaaacagga tctgcgacac catggccaag
     3241 gtatatgtgg acaaggaatt gtcatttgtg atcgactgcc cgccggagtt gacttggcgt
     3301 gtggacgaag gcgatgcgtt cgagattttg ggaaacttgc tcgataacgc cgctaaatgg
     3361 gcggtacacg aggtttgcct gcgaatctgg cttgacgagg atgccatgtg catgcgggtg
     3421 acggacgatg gcccaggttt cgctgatccg caagccaggc ttgctcgtgg tgtgcgaatg
     3481 gatgagtctg tgcccggcca tggcatcgga ttgactgtgg tcaacgatct ggtagccagc
     3541 tacctgggtg agttgaagat atccaatcgt ctcactgggg gggcccagat tgaaattgtg
     3601 ttgcccaaaa cttaggatac ggtcgcaaac agatgacgca ttcggcggtt gaatttggta
     3661 cgcaatgcca ggtgcattgc gtgacatctg gcgagacaaa ctagcggttt gaaacgacgc
     3721 agtgcgtcca agcctgacgg gccaaatggg aaagttattg tgtgacggcc atggccttca
     3781 ctcggccact tgtcagcaac gattttgagc aaaaagtgtt ccggcacagc cgctgatctg
     3841 tcttggcgca ggttgaggtt ccaggccgag ccaggcattg atggactggc aaagttgcca
     3901 gtcatctcgc cacggcggct tgcctgcacc aggctgcata gtgcgcaatc gccggattca
     3961 attgcatgca tggttaccgg cggtatgggt tgcctggccg gtgatcgaag cggttgcgaa
     4021 taccgtctcg gtcgagatcg cggtaagaac cgtgcagact ggcatggtgc aaatgactgc
     4081 gcactctgcc tgcttcgtaa atgttggcga tgccattgtg gttgtagtca ccgtacgggc
     4141 cgcggtgctg ccagtgccgc actgtattgc gtcgagcaaa gttatgcagc cgatagtagg
     4201 tggttgcagc agtgtagata gtgcgtgaac gaaaacgcca atctccctgg ggaaacctgc
     4261 cgaa
//
LOCUS       DAOSLL010000426         4259 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18320_length_4259_cov_4.439343, whole genome shotgun sequence.
ACCESSION   DAOSLL010000426 DAOSLL010000000
VERSION     DAOSLL010000426.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18320_length_4259_cov_4.439343"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="PLB25_16240"
     CDS             <1..418
                     /locus_tag="PLB25_16240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HOE43159.1"
                     /translation="RIFVATAAVADWRPEQAADQKMKKEGSGLPPVLRFAENTDILAT
                     LAQSPQASRGELFCVGFAAESHDLLAHAQAKRLRKGVPLLVGNIGPATFGQDDNALLL
                     VEATGTQEFARDSKLALARKLIAIIADRVRSGGQNC"
     gene            415..861
                     /gene="dut"
                     /locus_tag="PLB25_16245"
     CDS             415..861
                     /gene="dut"
                     /locus_tag="PLB25_16245"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HOE43160.1"
                     /translation="MNIEVKIIDPRMAGQLPAYATPGSAGLDLRACLNEPLTLPANAW
                     QLVPTGIAIYLKDPGFAALILPRSGLGHKHGIVLGNLVGLIDSDYQGQLMVSAWNRSD
                     TAFTIEPMERIAQLLIVPVLQAQFTVVSEFAASERGEGGYGSTGKA"
     gene            complement(1270..1416)
                     /locus_tag="PLB25_16250"
                     /pseudo
     CDS             complement(1270..1416)
                     /locus_tag="PLB25_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013212867.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            1498..1791
                     /locus_tag="PLB25_16255"
                     /pseudo
     CDS             1498..1791
                     /locus_tag="PLB25_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            2061..2624
                     /locus_tag="PLB25_16260"
     CDS             2061..2624
                     /locus_tag="PLB25_16260"
                     /inference="COORDINATES: protein motif:HMM:NF015827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HOE43161.1"
                     /translation="MKSQASACSRTPLGTLVALVMTMAAATAQAAPPIVSADDYCRDT
                     GGKVETYRFYDLSDAEFGKPAWVCNYPDASGTDPSGHAIIDLFTLYAENPTRAALAYY
                     SKLAWDGKLTVTPAKDYCLQLGGLPSFSGSWRLKPNSGESGMCMFEDGSYMEEWTLFY
                     NQSGTPRGVDLRTVMRFLMRIPIPVEP"
     gene            complement(2687..2880)
                     /locus_tag="PLB25_16265"
                     /pseudo
     CDS             complement(2687..2880)
                     /locus_tag="PLB25_16265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010814278.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            3529..4089
                     /locus_tag="PLB25_16270"
     CDS             3529..4089
                     /locus_tag="PLB25_16270"
                     /inference="COORDINATES: protein motif:HMM:NF038013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HOE43162.1"
                     /translation="MAEQKLGNPAVVGLAGFGFTTFILQFHNVGWMGIGPVIWLGLIF
                     GGLAQMIAGLQEMKTGNNFGYCAFTAYGCFWISLALILIGNHFNIYVSSATDIGWFLV
                     AWTGLTAILWVGSMRISGALGLTFTLLLIGFVLLDLAHFGFPQLTVVAGYELMVTAAC
                     AWYIMAHIIFADVFGRDVLPVGKPWI"
BASE COUNT          851 a         1220 c         1307 g          881 t
ORIGIN      
        1 tcgcattttt gtagcgacag cggcagtggc cgactggcga ccggagcagg cggcggatca
       61 aaagatgaaa aaggaagggt ctggcctgcc accggtcttg cgctttgccg aaaacaccga
      121 cattctggcg acactggcgc aatcaccgca ggccagtcgg ggtgagctgt tttgtgtcgg
      181 ctttgccgcc gaaagccacg acctgctggc ccatgcccag gccaagcgac tgcgcaaggg
      241 tgtgccattg ctggtcggca acattggacc ggccaccttt ggtcaggacg acaacgcctt
      301 gctgttggtt gaagcgactg gcacgcagga gtttgcgcgt gacagcaagt tggcgttagc
      361 tcgtaaatta atagctatca tcgcagacag ggtaaggtct ggaggccaaa attgttgaat
      421 attgaggtca agatcatcga cccccgcatg gctggccaat tgccggccta tgcgacacct
      481 ggcagcgccg ggctggactt gcgcgcctgt ctgaacgagc ccctaacgct gccagccaac
      541 gcctggcaac tggtgcccac cggcattgcc atttacctga aagacccggg ttttgccgcg
      601 ctgatcctgc cgcgctccgg gctgggccac aaacacggca tcgtgctggg caacctggtc
      661 gggctgatcg acagcgacta ccaggggcaa ttgatggtca gcgcctggaa ccgcagcgac
      721 accgcattca ccatcgaacc gatggagcgc attgctcaac tgctgatcgt gccggtgctg
      781 caggcgcagt tcacggtggt cagcgagttt gccgcgagcg agcgcggcga gggcggctac
      841 gggtcaactg gcaaggcctg aaggtttatc agatggtgtc gcagtcgtgg ggtggttgcg
      901 cgattgcgcg agggcacttg cggctcacct ggcagggcca gagccaaggt gtcgagcaaa
      961 aacagcttga acagttcggc cgcgctgaca agcgcaacgc gttgatgcaa accgcttgtg
     1021 taatgagcct gcttgaccga atcaggttga tcatgttgac cagcttggcg catagggtgc
     1081 cagcgccatg cggtctgcga catcggccag atcaagccca agatcaagcg ataccggcat
     1141 ccggcaagct tggtaagctg cactattggt agctactggc gctttctgcg taagggctac
     1201 aggcctgttt ggctatgaac cctgagttac caccacggcc atcgctgttt ggttgacacc
     1261 gcaatcgacc tacgaaatct gcccggtcat gcctgaagcc aggtcgctca gaaagaaagc
     1321 ggccacgttg ccaaccgcgt caatcgttac attgcgctta agcggcgaag ccgccgccga
     1381 aatgccaagc agcttgccaa agtccttgat gctgctgggc agcgcgctcc caatccagcc
     1441 cgttcctgcc aatcccgtgc cgcccaggcg cccacaggca cccacaggca ccctcaggcg
     1501 catcacttgt gggcggtgtt gattgcccgc agctacgaga tgttgccgtg cccgaacttc
     1561 ggtgggccga tgcgcatcac aagcgttcat caggcatggc gccgatatcg tgcagatact
     1621 gaaccacatc ggggtggact cagaaccggc tgacatctca ctggcgcggg agtgccgctg
     1681 tgggatggct gtgatgcgca gatcggtgat ggtgtcgaaa tcgagccgga atgggatctg
     1741 gcagcacaac cggcaccggg ctttgccgtc gatcagcgca tcaattggtg aatcagtgaa
     1801 acggcgattt tgcgtcgcag cagggtggcc tgggtgcgcg gtcaacccaa atctggcctg
     1861 ctcgccagag ccttgccatt gagttgccgc gacggcccaa actgaacaag tcaaagctgc
     1921 caggatgccc aaaacccatg acatacttgc gctcacgcgg ttggatttcc tatccgcaga
     1981 gcagcgctgc gcatcccgtg gccatgggcg acgctgagca gcgctagata taaacccttc
     2041 gcaatcacat ggagacgacg atgaagagtc aagcatccgc ttgcagccgc acacctttgg
     2101 gcaccttggt ggccctggtc atgaccatgg cggcagcgac cgcgcaggcc gcacccccaa
     2161 tagtatcggc tgatgactac tgcagagaca cgggcggcaa ggtggaaact tatcgctttt
     2221 atgacctttc ggacgctgaa tttggtaagc ccgcgtgggt ttgcaattat cccgatgcgt
     2281 ccggtacgga ccccagtggt catgccatca tcgacttgtt cacgctctat gccgaaaatc
     2341 ccacgcgggc agcgttggcc tactacagca agctcgcgtg ggacggcaaa ctcacggtca
     2401 cgccggccaa ggactattgc ctgcaactcg gcgggctgcc gtcgttcagt ggcagttggc
     2461 gactcaagcc gaactccggg gagtcgggca tgtgcatgtt tgaggatggc tcttacatgg
     2521 aagagtggac gctgttttat aaccaaagcg gaacgccgcg cggtgtcgat ctgcggactg
     2581 tgatgcggtt tctgatgcgg attcccatac cggtcgagcc ttgaggccat ccgccacacc
     2641 cacattcggc acttgtcgcg gtcgccccgg aatcaatcca gtcgttcttg atggtgccgg
     2701 cgctcaagct gttgacacgg atgccacggg caccgactgc atcggcgagg tcaagcactg
     2761 accactccag cgatgccttg gcaggcccag ggtgtcgtag ctcggcacca cacgcacgcc
     2821 tcccatgtag gtcaaagtca ccaaccaagc ggtgttgttc aagtagggca gatgcgcctg
     2881 accgccgtta ttgcccacag cgctgatatc cggcaaatgc taggtcacag cggggtggac
     2941 tcgcagcccc gcccgcgcgc ccgcacatag tccgggcgcg cgggcggccg cggtggggcg
     3001 acggcgatgc gcagatcagc gaggatgtta cgttcgagcc gaaccgggct ctgacagccc
     3061 aacccgcgcc agaccacgag gccggcagac aagtcaatgg gtgcgtgagt agagcggcga
     3121 ttggctggac ttgccgtggg gttgtgttgc gctgggtaca cgccgcacac cgcaaatcac
     3181 ccttctgaac acaaagccct ggtcgtgaat ttggcacgcc cggcggccaa ttcagggttt
     3241 tacgcgggcc gagggcggtt ccagaatgtg gtgcctgggc agttgaattt ccgattcatt
     3301 cgggttgttc ctgcctgacc aacatcgcca tattgacggc ccaaatgggt cggcctgggc
     3361 ggcatctgct gtatgtcgga ttgccgtaat gttcgacagg taccatttgg cggtgagcat
     3421 gagaggcaac tcttgttgca acgcgtcagc aaatgcggct tatccatcca ggtggccgca
     3481 ggcttgattg catgcttgca tgatttgatc aactacagga accaacatat ggcggaacag
     3541 aaattgggaa acccggcggt cgtcggactt gcgggctttg ggttcacgac gtttatcttg
     3601 caattccaca atgtggggtg gatgggtatc ggaccggtga tttggctggg gctgatcttt
     3661 ggtggcttgg cgcagatgat tgccgggctg caggagatga agaccggcaa caactttggc
     3721 tattgcgcct tcacggctta cggctgcttc tggatatccc tggcgctgat cctgattggc
     3781 aatcatttca acatttatgt gtcgagcgcc accgacattg gctggtttct ggtggcctgg
     3841 accggcctga cggcgattct gtgggtcggc tcaatgcgca tcagtggcgc cctggggctg
     3901 actttcacct tgttgctgat cggcttcgtg ctgctcgatc tggcgcattt cggctttcca
     3961 caactcactg ttgtagccgg ttatgaactg atggtgacag cggcctgtgc ctggtacatc
     4021 atggcgcaca tcatttttgc cgatgtcttt ggccgcgacg tgctgccggt cggcaagccc
     4081 tggatttaac cggttggcat cgggcaacgc accacatcaa caatgtcgct gcctggcgcg
     4141 gcgacccagc ctgggttgtc aacaaacagc tcacgcatct ttaatggaga aatgaaaatg
     4201 ctacatatca aaaattttgc gctggccttg ctggtttcaa tgtgcgctgg cgtggccgg
//
LOCUS       DAOSLL010000427         4256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18330_length_4256_cov_4.800048, whole genome shotgun sequence.
ACCESSION   DAOSLL010000427 DAOSLL010000000
VERSION     DAOSLL010000427.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18330_length_4256_cov_4.800048"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="PLB25_16275"
     CDS             complement(<1..375)
                     /locus_tag="PLB25_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013106512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HOE43163.1"
                     /translation="MSAPPVSRTLHAWIDGIGLIAPGLPDWSSAREVLQGKQAFAAAP
                     TVLPAPTLLPTAERRRASRVIKLCLGVGLQAVDHAGADAASLATVFASSGADGHNCHA
                     LCEQLAGDDRQISPTRFHNSVHN"
     gene            complement(372..1562)
                     /locus_tag="PLB25_16280"
     CDS             complement(372..1562)
                     /locus_tag="PLB25_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012761120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HOE43164.1"
                     /translation="MSPLLLSAYTLTTCLGRGLDATRDALHDERSGLAPCNFESVELA
                     TWVGEVAGVDAQVLPDRLRHYECRNNRLTQMGLETDGFDQRVRDAVARHGHDRVGVFL
                     GTSTAGIFQTELAYRGRDPVSGALPDSFNYRSTHNAFSLTEFTRDYFGLTGMAMTLST
                     ACSSSAKVFAAAARQLALGSIDAAIVGGVDSLCLTTLYGFASLQLTSAQPCRPYDVAR
                     DGISIGEAAAFALLERADRPAPGSVLLLGVGESSDAYHMSAPHPQGLGARLAMAAALR
                     SASLSEADIDYINLHGTATPANDAAEGQAVATLFGNGVPCSSTKGATGHTLGASGAVE
                     ALICALALTEGRLPGSPGTQVLDPTIRIRYQQHGAPARLRHALSNSFGFGGSNCSLIF
                     GVSP"
     gene            complement(2293..3375)
                     /gene="dctP"
                     /locus_tag="PLB25_16285"
     CDS             complement(2293..3375)
                     /gene="dctP"
                     /locus_tag="PLB25_16285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOE43165.1"
                     /translation="MDRRSIIKNAGIAGVLAAGIAPAVHAQAAVRWRLASSFPKSLDT
                     IYGAAEVMSKAVKAMSGGKFEISVHAGGELMPAFGVVDGVQNGTVEMTHTVPYYFYGK
                     NPAFALGSAIPFGLNARQMTAWMMHGNGRKLMNELYAGYGMTSFAGGNTGVQMGGWFR
                     KEIKSAADFKGMKMRLGGGLVGEVMQGVGAVPQSIPGGEIYQALEKGTIDAAEWVGPY
                     DDQKLGFNKVAPFYYYPGWWEGGPEVDFFVNQKAFDALSPENKAILENASAMAAADML
                     AKYDAFNPTALKQLVAAKTKVLRFPNDVLEAAFNSSMKVFEANNAKSPEWKKIYADLR
                     NFQRDQVLWFRFAEAKFDSFMATRKL"
     gene            complement(3598..>4256)
                     /locus_tag="PLB25_16290"
     CDS             complement(3598..>4256)
                     /locus_tag="PLB25_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HOE43166.1"
                     /translation="IHDVAIQNLPVVFALDRAGLVGADGATHAGAYDIAYLRCIPNIS
                     VACPADENECRQLLCTAFAQNTPVAVRYPRGNGAGVAVQPGLEALPFGKGEIRRQGQR
                     VAILAFGTLLYPALEVADKLNATVVNMRWAKPLDLALLLQVAAGHDALVTLEEGAVMG
                     GAGSAVLEALSQAGVLCQVLQLGLRDAFIEHGDPLKLLALQGLDAPGIEAAIRGRYFP
                     "
BASE COUNT          865 a         1313 c         1247 g          831 t
ORIGIN      
        1 gttatgcacc gagttgtgaa accgtgtggg cgaaatctgc cggtcgtcgc cggcgagttg
       61 ctcgcacagg gcatggcagt tgtggccatc ggcacccgat gaggcaaata ccgtggccag
      121 gctggcggca tcggcaccgg catggtcaac cgcctgcaag cccacaccga ggcacagctt
      181 gatcacccga ctggcgcggc ggcgctcggc cgttggcagc agcgttggcg ctggcagtac
      241 agtgggggca gcggcaaaag cctgcttgcc ttgcaacacc tcgcgtgcgc tggaccaatc
      301 gggcagaccg ggagcgatca ggccaatgcc atcgatccag gcatgcagcg tgcgactgac
      361 gggcggcgcg ctcatgggct cactccgaat atcaggctgc agttgctgcc tccaaaacca
      421 aaggaattgc tcagggcatg gcgtaaacga gccggtgcgc catgctgctg gtagcgaatc
      481 cgaatcgttg ggtcgagcac ctgggtaccc gggctgcccg gcaggcggcc ctctgtgagg
      541 gccagcgcgc agatcagtgc ttcgaccgcc cctgaagctc ccagcgtgtg gccggtcgcc
      601 cccttggttg agctgcacgg cacaccgttg ccaaacagcg tcgccacggc ctgcccctca
      661 gcggcatcgt tggcgggcgt ggcggtgcca tgcaggttga tgtagtcgat atcagcttcg
      721 ctcaaactcg cgctgcgcag cgccgccgcc attgccaggc gtgcgcccag gccctgtggg
      781 tgcggtgccg acatgtggta tgcatcgctc gattcaccca cgcccagcag cagcaccgac
      841 ccaggtgcgg gccggtcagc acgttcaagc agggcaaagg cggcggcctc gccaatcgag
      901 atgccatcgc gcgcgacgtc atagggccga cacggttgcg ccgatgtcaa ttgcaaggag
      961 gcaaatccgt acagcgtggt caggcacagg gaatccaccc cgccgactat tgccgcgtcg
     1021 atactgccca gcgccaactg gcgtgctgcg gcggcaaata ccttggcgct ggacgaacat
     1081 gcggttgaca gggtcatcgc catgccggtc agaccgaagt aatcgcgggt gaactcggtc
     1141 agcgaaaaag cgttgtgtgt gctgcggtaa ttgaagctgt caggcagcgc gccactgacc
     1201 gggtcgcggc cgcggtaggc cagttcggtc tgaaaaatgc ctgccgtgct ggtgccaaga
     1261 aaaaccccga cgcgatcatg cccatgccgc gccacggcat ctcgcaccct ttgatcgaac
     1321 ccgtcagttt ccagccccat ctgcgtcagc cggttattgc ggcattcgta atggcgcagc
     1381 cgatcaggca aaacctgcgc gtccacgcct gccacctcac ccacccaggt ggcgagttca
     1441 accgattcga aattgcaggg tgccagaccc gagcgctcgt cgtgcaacgc gtcgcgggtg
     1501 gcatcaagcc cgcggcccag gcaggtggtc agggtgtaag cagacagcag caaaggcgac
     1561 acaagatgaa atcgaaaaat tgaagctctg tatcttagtc gctgaaaaca gtgctaccag
     1621 cagttcaatg ggaagaattc ctggactgcc gaaacggcgc agatgcaccg ctgatggggc
     1681 aagactttgg gcatctgaaa actcgctgcg atagaactga ttcaaggctc cactatgagc
     1741 atcttgatcc tcactgagga tgcctttgtc agctttgcct gaatgaaagt gttgaacttc
     1801 atgaatcttt gaatgaaatc aatcagttgt ctcctttgat tcgcgagata catcgcccac
     1861 tgcgttgccc acactgtggt gcgactcgcc tgtggcatca cggctgttgc tttcgcaagg
     1921 ccgatcagcg cactgcgccg gatttgacac tcaatccggt accgatccag cgctacctgt
     1981 gccgcgcttg caaacacacc tgccaacctg cattgcaccc aggcgctggt ttgactgggt
     2041 catgcagcaa ctggttttgc tgatgctgtt ggcgggcaac tcggtacgcc tttgcagtgt
     2101 ttgcagctct cgcgcatgct ctatcgtgcg gcactggcac gactggccag gtgtgcgagg
     2161 tgagaccttc gctttctggc tgcgcagccg gttacctgaa ttgggtcagc accctgagcc
     2221 accatcattt tggagcctta ctggaacggc tagcgcagta gcggggcctt tagctggggt
     2281 caaccgaccg tctcagagct tgcgggtggc catgaaggag tcaaatttag cctcggcaaa
     2341 gcggaaccac agcacctggt cgcgctggaa gttgcgcagg tcagcgtaaa tcttcttcca
     2401 ttcagggctc tttgcgttgt tggcctcaaa taccttcatg gatgaattga aggccgcttc
     2461 cagcacgtca ttcggaaatc gcagcacttt ggtcttggcc gccaccaact gtttcagcgc
     2521 ggtcgggttg aaggcatcgt acttggccag catgtcggcg gcagccatgg cactggcgtt
     2581 ttccagaatg gccttgtttt ctggcgacaa ggcatcgaac gctttttgat tgacaaaaaa
     2641 gtccacctcg gggccaccct cccaccagcc agggtagtaa tagaacgggg ccaccttgtt
     2701 gaagccgagt ttttggtcgt cataggggcc gacccattcg gcggcatcaa tcgtgccttt
     2761 ttccagagcc tggtaaatct cgccgccagg gatgctttgc ggcaccgcgc caacgccttg
     2821 catcacctca ccgaccagac caccacccag gcgcatcttc atgcccttga aatctgcggc
     2881 agacttgatt tccttgcgga accagccacc catttgcacg ccggtgttgc cgccggcaaa
     2941 gctggtcatg ccgtaacctg catacaactc gttcatcagc ttgcggccat tgccatgcat
     3001 catccacgcg gtcatctggc gcgcattgag gccaaacgga atggccgaac ccaaggcaaa
     3061 cgccgggttc ttgccataaa agtaatatgg cacggtgtgg gtcatttcaa ccgtgccgtt
     3121 ttgtacacca tcgaccacac caaaagcggg catgagttcg ccaccagcgt gaacggaaat
     3181 ctcgaatttg ccgcccgaca tcgccttgac agccttggac atcacctcag ccgcgccgta
     3241 aatcgtgtcg agtgacttgg ggaagcttga agcaagacgc cagcgcactg cagcctgcgc
     3301 atgcacggcg ggtgcgatgc cagcggccaa tacgccagca atgcccgcgt ttttgatgat
     3361 tgaacgacga tccatggatt tactcctgag tttatgaaat gagatgctca ctgcgcggtc
     3421 agacccgacg gcaaaatgca tgcagcctgt ggattctata aacagcgccc catcaacaga
     3481 cacgggtttt cccttgagga atcagtgaaa acccgattga ctgaagaaac tgcgcgctgt
     3541 tgtttgcctt gcggcagctt tgcgccaatg tgttcagccg cgtgacacaa cccgatttca
     3601 ggggaagtac cgcccgcgaa tggcagcctc aatgccaggt gcatccagcc cctgcaaggc
     3661 cagcagtttc agcggatcgc cgtgttcgat gaatgcgtcg cgcaggccga gttgcaacac
     3721 ctggcacaac acgccggcct ggctcagggc ttccagtacc gcgctgcccg cgccacccat
     3781 gacggcacct tcttccagcg tcaccagcgc gtcgtgtcct gcggccactt gcagcagcaa
     3841 agccaggtcc agcggcttgg cccagcgcat gttgaccacc gtggcgttga gcttgtcggc
     3901 cacttcaagc gccggataca gcagcgtgcc aaaggccagt atcgccaccc gttggccctg
     3961 gcgacgtatc tcgcccttgc caaaaggcaa cgcctcaagt ccgggctgca ccgccacacc
     4021 ggcaccgttg ccgcgcgggt agcgcaccgc taccggtgtg ttttgcgcaa atgccgtgca
     4081 caacagttga cggcattcgt tctcgtcagc cgggcaagcc acgctgatat tcggaatgca
     4141 gcgcaggtag gcgatgtcat aggccccggc atgggtggca ccgtcggcgc caaccaggcc
     4201 ggcgcggtcc agcgcaaaca ccaccggcag gttctggatc gccacatcgt ggatca
//
LOCUS       DAOSLL010000428         4249 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18366_length_4249_cov_4.448736, whole genome shotgun sequence.
ACCESSION   DAOSLL010000428 DAOSLL010000000
VERSION     DAOSLL010000428.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18366_length_4249_cov_4.448736"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="PLB25_16295"
     CDS             complement(<1..464)
                     /locus_tag="PLB25_16295"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HOE43167.1"
                     /translation="MTEPSADFTAWIGRQETTEDDISLAPAIAAAAMLDDCQISLEKG
                     QSLPSLWHWLYFLPKAAQSNLGADGHPQRGGFMPPIPFPRRMFAGARLRFHHPLLIGQ
                     PARRVAEIRDIKQKSGKSGSLAFVSVLYRFYQGEVLCVEEEQDIVYREPGAPV"
     gene            631..2106
                     /gene="rng"
                     /locus_tag="PLB25_16300"
     CDS             631..2106
                     /gene="rng"
                     /locus_tag="PLB25_16300"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HOE43168.1"
                     /translation="MQEEILINWSPQETRVALVENGVLQELHVERTLERGLMGNVYLG
                     KVARVLPGMQSAFIDIGLDRAAFLHVADVWHPPADGETLSAARTAATLTPIEKQVFEG
                     QSLVVQVIKDPIGTKGARLSTQISIAGRMLVFLPQDCHIGVSQKIPSAQREELRRRMQ
                     QLVGDHGGGFILRTNGEDASDKELSDDIDYLCKAWTRIREAAARLPVTSLLHQDLNLV
                     QRVLRDLASDLTQSIKVDSLEQFENLKAFGQEFMPTATQKLQHYKGERPIFDLHSIDE
                     EIAKALGRRVELKSGGYLIVDQTEALTTVDVNTGGFVGARNFDDTIFKTNLEAAQAIA
                     RQLRLRNLGGIIIVDFIDMVREDHREAVLAEIRKQLSRDRVKTMCGGFSQLGLVEMTR
                     KRTRESLAHMLCEPCPLCVGKGILKTARSVAYDIFREILREARQFDPREFRVVASPKV
                     IELFLDEESQYLAGLSEFIGKPVSLQSENAMGQEQYDIVLM"
     gene            complement(2130..2999)
                     /locus_tag="PLB25_16305"
     CDS             complement(2130..2999)
                     /locus_tag="PLB25_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE43169.1"
                     /translation="MTVSISGFTFIRNGVTLDFCFEASIRSLLPLVDEFVIAVGNSSD
                     DTLNRVRAIGDPKIRILETIWNDRMADRGFVYAQQKMIAQYACTGDWAFYLEGDEVIH
                     EDELTSIRASIEQHHPNPEVEALVFDYYHFYGSCDWIAVSPAWYRRECRLIRNTIRSY
                     APDGQYWLVTSQHKRPRNPKAALANAHIFHYGWVRRNEDMNKKLTQVSKYWGTPAAIV
                     TYGQFDARALKPFKGTHPQVVQAWLTTSAEQTLRIDPGYRPTGKENKHHLMQRIENFT
                     GLDFSRKHFKLVA"
     gene            complement(3031..>4249)
                     /locus_tag="PLB25_16310"
     CDS             complement(3031..>4249)
                     /locus_tag="PLB25_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE43170.1"
                     /translation="VMRVLSARLYTLTKASQTATDNLAYVVEENVLAHRDIRLHAAQP
                     VQLKRFSELSHSLRRLSIKSSVASAAMTPITQMLAAVALSTVIVMALVQSEGNTNSVG
                     SFVAFVTAMLMLIAPIKHLSEVASPITRGLAAIERGLTLLETTPSESEGDFSMLRSQG
                     NLCFSNITVSFGSDAAPAVKDLSLTVNPGETVALVGPSGAGKTTLVNLLPRFIDATSG
                     TITLDGHDTRDWRLDSLRSQFAVVSQHVVMLSGSIAFNVALGQEIDRQKVNDSLRAAN
                     LLNFVHELPNGIDTDVGHNAMQLSGGQRQRLAIARALYKNAPILILDEATSALDSESE
                     RAVQDALENLMKSRTTLVIAHRLSTVHRANRIVVMDAGQIIEIGSHAQLLQRNGQYAR
                     LYQLGLNTQPIGS"
BASE COUNT          949 a         1098 c         1203 g          999 t
ORIGIN      
        1 actggcgcac caggctcgcg atagacgatg tcttgctctt cttcaacgca gagcacctca
       61 ccctggtaga aacggtaaag caccgagaca aacgccagcg aacccgattt gccggatttt
      121 tgcttgatgt cgcgaatctc tgccacgcgc cgggccggtt ggccaatcag caacggatga
      181 tgaaaacgca gtcgagcgcc ggcaaacatg cgccgaggga agggaatcgg tggcatgaag
      241 ccgccgcgct gcggatggcc gtcagcgcca agattggact gtgctgcctt gggcaaaaaa
      301 tacagccagt gccacaaact tggcaacgat tggcctttct cgagtgaaat ctggcagtcg
      361 tcaagcatcg ctgcagcggc tatggcaggt gccaggctga tatcgtcttc ggtggtttcc
      421 tggcggccta tccatgccgt gaaatctgcg gatggttcag tcattcatca gtcctttcag
      481 ttttgttttc gcacgggtgt gaggtaaagc acaaagctct tcaagcttgc aggtgtgcca
      541 atctccaaat ttgtgccaga tattgtctct cgaccctttg gccctgtgtg ccaaagtcca
      601 aatcattatt tccggtaaag tcggctgctt atgcaagaag aaattttgat caactggtca
      661 ccgcaagaga cacgcgttgc cttggtggaa aacggggtgc tccaggagtt gcacgtggaa
      721 cgtacccttg agcgcggcct gatgggcaat gtttatctgg gcaaagtggc gcgcgtattg
      781 cctggaatgc agtcggcttt cattgacatt ggtcttgatc gggcggcttt cttgcatgtc
      841 gctgatgtct ggcaccctcc tgccgatggc gagaccttgt ctgcagcgcg cactgcagca
      901 acgctgacac ccatcgaaaa gcaagtgttt gagggtcaaa gcctggtggt acaggtgatc
      961 aaggatccga ttggaacgaa aggggcccgc ctgtcaaccc agatcagcat cgccgggcgg
     1021 atgctggttt ttttgccaca ggattgccat attggtgtgt cgcagaaaat tccgagtgct
     1081 cagcgtgaag aactgcgtcg gcgcatgcag cagttggttg gtgatcatgg tggcggattt
     1141 attttgcgca ccaatgggga ggatgcgtct gacaaagagt tatcagacga tattgactac
     1201 ctttgcaagg catggacgcg catccgggag gctgccgcgc gtctgccagt tacatcgctt
     1261 ttgcatcagg atctgaatct cgtgcaacgt gtactgcgtg acctggccag tgacctaacc
     1321 cagtcgatca aagttgattc gcttgagcag ttcgagaatt tgaaggcctt tggccaggag
     1381 ttcatgccaa cggcgaccca aaaattgcag cactacaaag gcgagcgacc gatttttgac
     1441 ctgcattcaa tcgacgaaga aatcgccaag gcgcttggcc gacgtgtaga gcttaagtcc
     1501 ggtggctacc tgatcgtgga tcagaccgag gcgttgacca cggtggatgt caacaccggc
     1561 ggttttgttg gcgcgcgtaa ttttgatgac acgattttca agaccaatct tgaggccgcg
     1621 caagccattg cccggcagtt gcgcttgcgc aacttgggcg gcatcatcat cgttgatttc
     1681 attgacatgg tgcgcgaaga ccatcgcgag gcggtgctcg ccgaaatacg caagcagctc
     1741 tcgcgtgacc gtgtcaaaac catgtgtggt ggcttttcac aactgggatt ggtcgagatg
     1801 acgcgcaagc gcacccgcga gtcgctggcg cacatgctgt gcgaaccttg tccgctgtgt
     1861 gtgggcaaag gtattttgaa aacagcacgc agcgtcgcat acgacatttt tcgggaaatt
     1921 ttgcgcgagg ctcgccaatt cgatccgcgt gagttccggg tggtggcttc acccaaggtt
     1981 atcgagttgt ttctggacga ggaaagtcaa tatctggctg gcttgagtga gttcatcggc
     2041 aagccggttt cattgcaaag tgaaaatgcc atggggcagg aacagtacga cattgtgctg
     2101 atgtgaccac ctgaacttct ccgcgcccgt catgcaacca gcttgaagtg tttgcggctg
     2161 aagtccaggc cggtgaagtt ttcaattctc tgcatcagat ggtgtttgtt ttcttttccg
     2221 gtcgggcggt agcctgggtc aattcgcaag gtttgctctg cgctggtggt taaccaggcc
     2281 tggacaactt gcgggtgcgt tcccttgaat ggttttaagg cacgggcatc aaattgaccg
     2341 taagtgacga tcgctgcagg tgttccccaa tacttgctga cctgggtcag ctttttgttc
     2401 atgtcttcgt ttcgacgaac ccagccgtag tgaaaaatgt gggcattggc cagcgccgct
     2461 tttgggttgc gggggcgttt gtgttggctg gtgaccagcc aatactgccc gtcaggtgcg
     2521 tagctccgaa tggtgttgcg gattaaacgg cattcacggc gataccaagc gggactgacc
     2581 gcgatccagt cgcagcttcc gtaaaaatgg taatagtcaa acaccagcgc ttcgacctca
     2641 gggttggggt gatgctgctc aatactggca cggatgctgg tcagctcgtc ttcgtggatg
     2701 acctcatcgc cctccagata aaacgcccaa tcacctgtac atgcgtactg tgcgatcatt
     2761 ttttgctgcg catagacaaa gccacgatca gccatccggt cattccagat ggtctcaaga
     2821 atgcgaattt tgggatcgcc gattgcacgc actctgttca aggtgtcgtc gctgctgttg
     2881 ccgacggcaa tgacaaactc gtcaaccagt ggcagtagtg agcggatgga ggcttcaaag
     2941 caaaagtcaa gtgtcacacc attgcgaatg aaggtaaaac cactgatgga aacagtcatg
     3001 atgcgcgttt gaaacaggtt tattggagat ctaggaccct atcggctgcg tgttcagtcc
     3061 caactgatac aggcgtgcat attgtccatt gcgctgcaac aactgcgcat gcgagccaat
     3121 ttcaatgatt tgtcccgcat ccatgacaac aatgcgattg gcccggtgca cggtggacaa
     3181 gcgatgcgca atcaccagcg tggtccggga tttcatcaaa ttctccagcg cgtcctgaac
     3241 cgcgcgttca gactcggagt cgagcgccga agtcgcttca tcaagaatca agataggcgc
     3301 gtttttataa agtgccctgg cgatggccag tctctggcgc tgcccgccag acaattgcat
     3361 cgcgttgtga ccaacgtcgg tgtctattcc attgggaagt tcatgcacaa agttgagtag
     3421 attcgccgct cgcagactgt cgtttacctt ctgccggtca atctcctgcc ccagcgcgac
     3481 attgaacgcg atgcttccac tgagcatcac cacgtgctga ctgaccacgg cgaactggct
     3541 tcggagcgaa tcgagccgcc aatcccgcgt gtcgtgacca tcgagtgtga tggttcctga
     3601 agttgcatca atgaaccttg gcaataggtt caccagcgtc gtcttgccgg cgccagacgg
     3661 acccactagc gcaaccgttt cgccggggtt gaccgtcagt gacaaatcct tgacagcagg
     3721 tgcagcgtcg ctgccaaaac tcaccgtgat atttgaaaaa cacagattac cttgactgcg
     3781 cagcatggaa aaatcacctt cactttcgct gggtgttgtc tcaagcaagg tgagtcctcg
     3841 ctcaatcgca gccaggccac gcgtgatcgg gctggcaact tcggacaaat gcttgatggg
     3901 tgcaatcagc atcagcatgg ctgtcacaaa tgcgacaaac gaacccacgg aattggtgtt
     3961 gccttcactt tgaacaagcg ccatgacaat gaccgtagac agagcgacgg ctgccagcat
     4021 ctgtgtgatg ggtgtcatgg cagccgaggc aactgacgat ttgattgaca aacgacgcaa
     4081 tgaatggctc agctcggaga agcgcttgag ctgaaccggt tgcgctgcgt gcaatcgaat
     4141 gtcgcgatgc gcgagcacgt tttcctccac cacgtaagcc agattgtcgg tggcagtctg
     4201 actggccttt gtcaaggtgt aaaggcgcgc cgacaaaacc cgcatgaca
//
LOCUS       DAOSLL010000429         4242 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18403_length_4242_cov_3.708383, whole genome shotgun sequence.
ACCESSION   DAOSLL010000429 DAOSLL010000000
VERSION     DAOSLL010000429.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4242)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4242
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18403_length_4242_cov_3.708383"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="PLB25_16315"
     CDS             <1..582
                     /locus_tag="PLB25_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HOE43171.1"
                     /translation="VVTVPLILLAIPSVVIGFVTIGPMLFGDFLKDSILVNSEIHKAL
                     EILGEEFHGPVQLALHGLSAAPFWLALAGVVCSYYMYMVNPALPAAIKRSLMPLFSLL
                     ENKYYLDWFNENVLARGARGLGLVLWKVGDQALIDGACVNGSWRIVGWCSGIIRKVQT
                     GYLYDYALTMALGIFAMVTYFVTWPMAVQWLGK"
     gene            596..2071
                     /locus_tag="PLB25_16320"
     CDS             596..2071
                     /locus_tag="PLB25_16320"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HOE43172.1"
                     /translation="MGLLSLAIWTPILFGVVLLALGRDDQAQAVRWIALIGALVSLLV
                     TLPLYTKFDVTTAAMQFGEMSPWIERFSINYHLGVDGISIWFILLTAFINLVVVIAGW
                     EVITSRVNQYMGAFLILSGLMIGVFCAIDGLLFFVFFEATLIPMYLIIGVWGGPNKIY
                     AAFKFFLYTLLGSLLMLIALIYLYNVSGGSFDLAVWHKLKIDASAQTLLFFAFLAAFA
                     VKVPMWPVHTWLPDVHVEAPTGGSAVLAAIMLKLGAYGFLRFSLPITPDASHEWSGLM
                     IGLSLAAVIYVGLVTMVQKDMKKLVAYSSIAHMGFVTLGFFIFSDLGVAGGIVQMISH
                     GFVSAAMFLGIGVLYDRVHSREIASYGGVVNTMPKFTAFALLFFMANCGLPGTAGFVG
                     EWMVILGAVKFNFWVGLASASALIFGAAYSLWMFKRVYLGPVANEHVRHLSDINAREF
                     FFLSLLAVAVLYMGVYPKPFTDVMDVSVTELLKHVAQSKLH"
     gene            2112..3599
                     /gene="nuoN"
                     /locus_tag="PLB25_16325"
     CDS             2112..3599
                     /gene="nuoN"
                     /locus_tag="PLB25_16325"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HOE43173.1"
                     /translation="MIDKLSLIAVYPEILLLIMACVIAMFDLGVRDPNRTPTYILTLL
                     TLAAVAGLQASYASSGQTIYGFGNMVVSDAMGNWLKCFAALAVLVTLVYGRAYAVGRD
                     MLIGGELFTLSLFALLGMFVMISGNNFLVIYMGIELLTLSSYALVALRRDNGLAIEAA
                     MKYFVLAALASGFLLYGMSMLYGATGSLDLNEVSKAILTGQIKHQVLVFGTVFLVAGL
                     AFKLGVVPFHMWIPDVYQGAPTVVTLLIGGAPKLAAFAIVIRLLVGGMSGLAFDWQQM
                     LMFLAVASLAVGNFAAIAQTNIKRMLAFSTISHMGFVLIGLMSGVVSGHPDAAGNAYG
                     ASMFYVVSYVLTTLASFGIILLLSRKGFESEEISDFAGLNQRSPFYAGIMAMCLFSLS
                     GIPPMVGFYAKLAVLQALIASGSSSHIALAVFAVMMSLVGAFYYLRVIKVMYFDAPIT
                     ATAVTAPLEVRAVLTINGGLLLLLGLLPGGLMTLCAGAVLKMLAS"
     gene            3656..4234
                     /locus_tag="PLB25_16330"
     CDS             3656..4234
                     /locus_tag="PLB25_16330"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOE43174.1"
                     /translation="MQLIEKKLDSTELFQGKLLHAFRDTVELQNGAVATREYVVHPGA
                     VMVVPLMVDSSGDLSLVMERQFRYPVNRVMIEFPAGKLDPGEHSWHCARRELREETGY
                     RASFWAFAGTLHPVISYSTEFVDIWFAKGLTLGEQALDDGEFLDVFTASPAQLLEWCR
                     TGAVTDAKTMIAALWIQNVLSGAWTLDWKRDW"
BASE COUNT          762 a          909 c         1224 g         1347 t
ORIGIN      
        1 gtggtaacgg tgccattgat cctgctggcg attccttctg tcgttatcgg ttttgtcact
       61 atcggcccga tgttgttcgg tgactttctt aaggactcga ttttggtgaa ctcagagatt
      121 cacaaggcct tggaaattct tggggaagag tttcatggcc cggttcaact tgcattgcat
      181 ggcctatcgg cggccccgtt ttggctggcc ctcgctggtg tggtttgttc gtattacatg
      241 tatatggtga atcctgcact gcccgcagcg atcaaacgaa gtctgatgcc gttgttcagt
      301 ttgttggaaa acaagtacta cctggattgg ttcaatgaaa acgtgcttgc tcgaggtgca
      361 cgtggccttg gattagtcct gtggaaggtt ggcgatcaag ccttgattga cggcgcttgc
      421 gtcaatggct cctggcgcat tgttggctgg tgttctggca tcattcgaaa ggtgcagaca
      481 ggatatctgt acgactatgc cttgacgatg gctcttggta tctttgcgat ggtgacgtac
      541 tttgtgactt ggcccatggc tgtgcaatgg cttggtaaat agggagaaga agaaaatggg
      601 tttgttgagc ctggctattt ggacaccaat tttatttggt gtcgtgttgc ttgcattggg
      661 tcgggatgac caagcacagg cggttcgctg gattgcgttg atcggtgcgt tggtcagttt
      721 attggtcact ttgccgctct atacaaagtt tgatgtgacc accgctgcca tgcaatttgg
      781 tgaaatgtcg ccatggatcg agagattcag tatcaactac cacctcggag tcgacggtat
      841 ttctatttgg tttatcttgc tgactgcttt tattaatttg gtggttgtga tcgctggatg
      901 ggaagtcatt accagtcgtg tcaatcaata catgggcgct tttctgattt tgagtggtct
      961 catgatcggt gttttttgtg ccattgacgg gctgctgttt tttgtatttt ttgaggccac
     1021 tttgatcccg atgtacctga tcattggtgt ctggggcggg cccaacaaga tttatgccgc
     1081 tttcaaattc tttttgtaca cacttctggg ttcgctgctg atgctgattg cgctgattta
     1141 tctgtacaac gtgtctggtg gcagttttga tctggctgtc tggcacaagt tgaagataga
     1201 cgcgagcgcg cagactttgc tgttttttgc ctttttggct gcctttgccg taaaggtacc
     1261 aatgtggccg gttcatactt ggttgcctga tgttcacgtt gaagcgccaa ctggcggttc
     1321 ggcagttctt gctgccatca tgctcaagtt gggtgcctat ggttttctca ggttttcatt
     1381 gccaatcacg ccagatgcct cgcatgaatg gtccggtttg atgattggtc tttccttggc
     1441 tgcggtgatt tacgtgggac tcgtcaccat ggtgcagaag gacatgaaaa agctggtcgc
     1501 atattcatct atcgcacaca tgggctttgt tacgctcggg ttttttattt tcagcgacct
     1561 tggtgtggct ggcggcattg ttcaaatgat ttcgcacggg ttcgtctccg ctgcgatgtt
     1621 cctgggaatt ggtgtgcttt acgacagagt gcactctagg gaaatcgcta gctacggagg
     1681 cgttgtcaac actatgccca aattcacagc atttgccctg ctgtttttta tggcgaattg
     1741 tggattgcct ggaaccgcag ggtttgtcgg tgaatggatg gttattctgg gtgcggttaa
     1801 attcaatttt tgggttggct tggcttccgc ctcggcattg atatttggtg ccgcctattc
     1861 gctctggatg ttcaagcgtg tttacttggg cccggtagcc aacgaacacg tcaggcatct
     1921 ttctgacatc aatgcgcgcg agttcttttt cctgagtttg ttggcagttg ctgttttgta
     1981 catgggtgta tatcccaagc cgttcactga tgtcatggat gtgtcggtta cagagttact
     2041 caaacacgtc gcgcagtcaa agttgcactg atcgtccagt atggatcgct acctagagat
     2101 aagagattta aatgattgac aaactcagtt tgatcgcggt ttaccccgag attcttcttt
     2161 taatcatggc gtgtgtgatt gccatgtttg acttgggtgt cagggacccc aatcgaaccc
     2221 cgacttacat ccttaccttg ctaaccttgg ctgctgtagc gggcttgcag gccagttacg
     2281 ctagtagcgg tcaaacgatt tacggctttg gcaacatggt tgtgagtgat gcgatgggca
     2341 attggcttaa gtgctttgcg gctctcgcgg tactagtgac tttggtttat ggtcgtgctt
     2401 atgcggtagg gcgggacatg ctgataggcg gtgaattgtt taccttgagc ctgtttgcat
     2461 tgctgggcat gtttgtgatg atttccggaa ataatttctt ggtcatttac atgggcattg
     2521 agttgcttac cttgtcgagc tacgccttgg tcgcgttgcg gcgtgacaac ggcttggcca
     2581 ttgaagcagc catgaagtat tttgtgcttg cggcgctggc ttcgggcttc cttctttacg
     2641 gcatgtccat gttgtacggt gccactggtt ctcttgacct gaacgaggtt tccaaagcga
     2701 tcttgacagg acagatcaag catcaggtgc tggtgtttgg aactgtgttt ctggtagctg
     2761 gtctggcttt caagctcgga gtcgtgccat ttcacatgtg gattcccgac gtttaccaag
     2821 gtgcaccgac cgtcgtcacg ttgttgattg gtggcgcgcc aaagctggct gcttttgcga
     2881 tcgtgattcg tttgttggtc gggggaatgt ccgggctggc gttcgattgg cagcagatgt
     2941 tgatgttcct ggcggtcgct tctctggcgg tgggtaattt tgctgccatt gcgcaaacca
     3001 atatcaagcg aatgcttgcc ttttcgacca tctcgcacat gggttttgtc ttgattgggt
     3061 tgatgtcggg ggtggtgagc gggcatcctg acgctgctgg caatgcctat ggtgcttcaa
     3121 tgttttatgt ggtgtcgtat gtgctgacaa cccttgccag cttcggcatt attttgctgt
     3181 tgtcccgtaa gggattcgag agcgaggaaa tttctgattt cgctggtttg aaccagcgaa
     3241 gcccgtttta tgctgggatc atggccatgt gccttttttc actgtccgga attccgccaa
     3301 tggtcggctt ttacgccaag ctggcagtgt tgcaggcatt gattgcttcg ggcagcagtt
     3361 cgcatatcgc cttggctgtg tttgcagtga tgatgtcgct tgtcggcgcg ttctattatt
     3421 tgcgggtcat caaggttatg tattttgatg cgcccattac tgcaactgct gtgacagcgc
     3481 cgcttgaagt tcgtgccgtg ctgacgatca atggtggatt gcttttgctt ctaggcttgt
     3541 tgcctggtgg tttgatgacg ctgtgcgctg gtgccgtttt gaagatgctg gcaagctgat
     3601 gccaggaacc agtacctgat catttctttg acttgctggc tcttccatcg gcgtggtgca
     3661 acttattgaa aagaagctag acagtactga actgtttcag ggcaagcttt tgcacgcctt
     3721 tcgtgacacg gttgaactcc agaatggagc ggttgcgacc cgtgagtacg tcgttcaccc
     3781 tggtgcggtc atggtcgttc cgctgatggt tgactcgtcg ggtgacttgt cgttggtgat
     3841 ggagcgacag tttagatatc cggttaatcg ggtgatgatc gagttcccgg ctggcaagtt
     3901 ggaccctggt gagcactctt ggcattgcgc aaggcgcgaa ttgcgtgagg aaactggcta
     3961 ccgcgccagt ttttgggctt ttgccggaac tctgcacccg gtgatttcct actctaccga
     4021 atttgttgat atttggttcg ccaaagggct gactcttggc gagcaggctt tggatgatgg
     4081 agagtttctg gatgtgttta ctgcgagtcc cgctcagtta ctggaatggt gccgcactgg
     4141 tgcggtgacc gatgccaaaa ccatgattgc tgcgctgtgg attcaaaatg tgctttcagg
     4201 tgcttggacg ctggactgga agcgtgattg gtaactggca gg
//
LOCUS       DAOSLL010000430         4226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18498_length_4226_cov_3.782306, whole genome shotgun sequence.
ACCESSION   DAOSLL010000430 DAOSLL010000000
VERSION     DAOSLL010000430.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4226
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18498_length_4226_cov_3.782306"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(30..470)
                     /locus_tag="PLB25_16335"
     CDS             complement(30..470)
                     /locus_tag="PLB25_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOE43175.1"
                     /translation="MFKHILLATDGSAASEHAAKLAVSLARTHGAKLTALYVADPYPY
                     IGVGEINPMGYQAYSAAAQQLAAQAHSTVDALCKQGGSAVALQARLVEDVAAASGIVQ
                     SAKDEGADLIVMGSHGRSGIARLMLGSVATKVVAESPVPVLVTR"
     gene            complement(542..1516)
                     /gene="ylqF"
                     /locus_tag="PLB25_16340"
     CDS             complement(542..1516)
                     /gene="ylqF"
                     /locus_tag="PLB25_16340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HOE43176.1"
                     /translation="MAIQWFPGHMHLTRKAIAERIKDIDVVIELLDARLPGSSANPML
                     AEMTAAKPRLMVLNKQDLADPVRTEEWLAHYNAMPGVRAIALDASVATPARALVTACH
                     ALAPNRGGMAKPMRVLICGIPNVGKSTLINTLKGKRAAKTGDEAGVTKLEQRITIADD
                     FYLYDTPGVLWPRIIVAKSGYNLAASGAIGRNAFNEEEVALELLDYLRQHYAHAVETR
                     YKLQAVEVQTDEQLLDAIGRKRGALQGGAKVNLQKAAEIVIHEFRAASMGRITLETPG
                     EFAQWLAQGQKLDAERQVKKDAIEHARLVRLKKIPRPAKDGDRGDAPS"
     gene            1697..2230
                     /locus_tag="PLB25_16345"
     CDS             1697..2230
                     /locus_tag="PLB25_16345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43177.1"
                     /translation="MTSKSLTTVTNELIESYGNTAKNVINAYRAGNERAVGFVDHSWA
                     TAVQKAGTRLSAEVRGNALSAQKKISGLYTNAVTLTSDGADVAVNKAVEIAGKGVKQV
                     AANASRFEKATGVTALNTLAVAAVPAAQAVVEVATKLEAQSGALVNKVAGSKTKVKVA
                     AVKRTVAKKAARARKAA"
     gene            2300..2632
                     /locus_tag="PLB25_16350"
     CDS             2300..2632
                     /locus_tag="PLB25_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOE43178.1"
                     /translation="MVPLESRIRRDGRQVHELEKSLGFALLKPSGRGVFLTETGMAAM
                     RLAIGVSDSLPKPATRKRLQPIIGVPSLRLLRHEDEFERLQGDLAVHQLDMVLSDRPA
                     PTNPNIKL"
     gene            complement(2877..3305)
                     /locus_tag="PLB25_16355"
     CDS             complement(2877..3305)
                     /locus_tag="PLB25_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HOE43179.1"
                     /translation="MSSLISRGSLFDDFFRDVAPGFYVKSLHGDALPTAASIKVDVKE
                     TDKGYTVHAEVPGVNKEDIHVSLEGNVVTVRAEVKQQDSQTQDEKVLRSERYFGAVSR
                     SFQLPSDIDQESAKAKYDNGVLVLTLPKKLGGAAQRLAID"
     gene            complement(3456..>4226)
                     /locus_tag="PLB25_16360"
     CDS             complement(3456..>4226)
                     /locus_tag="PLB25_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HOE43180.1"
                     /translation="PAYEAIGVQGTDGEYRQLATSLLQIENEFYGTIRPKRVIHAGER
                     PLHALRERGVEYVEVRLMDLNPFEPLGISAQTMRFLDVFLVYCLLSDSPPDTPAEIAA
                     IAANQQRVAERGREPGLQLQRGGSEISLAAWGNDLVQAMEPIALQFDTAHGATDYMVA
                     LQAAKLGLAQPETLPSARVVQTIESEYGGSFVAFARAQSEGTKNAMLEVPLPDSVRTR
                     LEAQSATSIQEQQDIAAADSMPFDVYLREYLSPARLGV"
BASE COUNT          899 a         1296 c         1148 g          883 t
ORIGIN      
        1 ccaccaccaa gaacctgtaa ggtttgcgct tagcgggtga ccagcaccgg caccggcgat
       61 tcggcaacca ccttggtcgc cacacttccg agcatcaggc gagcgatgcc ggagcgtccg
      121 tgagacccca tcacgatgag gtcagcgccc tcgtccttgg cactttgcac aatgccactg
      181 gccgcggcaa cgtcttccac cagacgtgcc tgcaaagcca ccgcagatcc accctgcttg
      241 cacagcgcat ccacggtgga gtgcgcctgg gctgccaact gctgtgcagc ggcggaataa
      301 gcctggtacc ccatgggatt gatctcacct acgccaatgt agggatacgg atcggccacg
      361 tacagcgcag taagttttgc gccgtgggta cgcgccaggc tgaccgcaag tttggccgcg
      421 tgctcagaag cagcagaacc gtcggtggcc aacagaatat gtttaaacat ggatgcctcg
      481 catggtggtt gggttatcgt tcactgtaac gaccatcgcc catttgcgtc cttgatgcaa
      541 gtcaacttgg tgcatcgcca cgatcaccat cctttgccgg tcgcggaatt ttcttgagtc
      601 ggaccagccg ggcatgctca atagcatctt tcttgacttg gcgctctgca tcgagcttct
      661 gtccctgtgc gagccattgg gcaaattcac caggtgtctc cagcgtgatg cgccccatgc
      721 tggccgcccg aaactcgtgg atcacgattt cagcagcttt ctgcagattc accttggcac
      781 cgccctgtaa ggctccgcgt ttacgaccga tggcatccaa caactgttca tcggtctgaa
      841 cttcgaccgc ttgcaacttg tatcgggtct ctactgcatg tgcgtaatgc tgtcgcagat
      901 agtccagcaa ctccagcgcc acctcttctt cgttgaaggc attgcgtccg atagcgccac
      961 tggccgccag gttgtagcca ctttttgcaa cgatgatccg tggccagagc acacccggtg
     1021 tgtcatacaa atagaaatcg tcggcaatcg tgatgcgttg ttccagtttc gtcaccccgg
     1081 cctcatcgcc ggtttttgcc gcgcgcttgc ccttgagcgt gttgatcagt gtggattttc
     1141 caacattcgg aatgccgcaa atcagcaccc gcatgggctt ggccatgcca ccgcggttgg
     1201 gcgccagcgc gtggcacgca gtcaccagcg cccgcgcagg ggtggcaaca ctggcatcca
     1261 atgcaatggc gcgcaccccg ggcatggcgt tgtaatgggc aagccactcc tcggtccgca
     1321 cagggtcggc aagatcctgt ttgttgagca ccatgagccg gggctttgca gccgtcattt
     1381 cagccagcat ggggttggca ctggaccccg gcaggcgggc atccagcaac tcgataacaa
     1441 cgtcaatgtc cttgatgcgc tccgcaatcg ccttgcgtgt cagatgcatg tgaccgggaa
     1501 accattgaat tgccatacgc gtgaagaaac tcgtttgtta ccaagcgctc cattgtcggg
     1561 cagtttggat aagtcgggaa cctaggcagc tacctctagg aaacaacccg aatttaaggt
     1621 gctttcacta ggacatcttc cgagaattgc acccaccagt tcacacctgt tttctattca
     1681 cactctggag aattccatga cttccaaatc actgaccacc gttaccaacg aactcatcga
     1741 gtcttacggc aataccgcca agaatgtcat caacgcctat cgtgctggca atgagcgtgc
     1801 cgtcggattc gtcgaccaca gctgggcaac cgctgtccag aaggccggca cgcgcctgag
     1861 cgccgaagtt cgcggcaatg cgctgtcagc acaaaagaag atttccggtc tgtacaccaa
     1921 cgctgtcaca ctgacctctg acggtgcgga tgtggccgtt aacaaggccg tcgaaatcgc
     1981 tggcaagggt gtcaaacaag tcgccgcaaa cgcaagccgc tttgaaaaag ccaccggcgt
     2041 aaccgccctg aacaccttgg ccgttgccgc agtaccggct gcccaagccg tggtggaagt
     2101 ggcaaccaag ctggaagccc agtccggtgc actggtcaac aaggttgccg gttccaagac
     2161 aaaggtcaag gttgctgccg tcaagcgcac tgtggccaag aaagccgcac gcgcgcgcaa
     2221 ggctgcgtaa ttgcttttct gcctgcttcg cgggcagcac acaggcatct gccgcaccag
     2281 cggcaggtgc ctgttttcct tggtgcccct cgaatcgaga atccggcgcg acggcaggca
     2341 ggtgcatgag ctcgaaaagt ccctgggctt cgcactcctg aaaccctccg gccgcggcgt
     2401 attcctgaca gagaccggca tggcagccat gcggctggcg atcggcgtat cagacagcct
     2461 gcccaagccg gctacgcgca aacggctgca acccatcatc ggagttccct cactgcgcct
     2521 cttgcgccac gaggatgaat ttgaacgtct gcaaggcgat ctcgctgtgc accagctcga
     2581 catggtgttg tctgaccgtc cggccccgac caacccgaac atcaagctct aaagccatcc
     2641 catggggtca tcggacgttg cctggaatgt gcccgccgca ctggtgctga atcgccccgg
     2701 ggcgattcag tgtgtttccg ttagtggcga tgctgcacga cgaacttcgg acccactacg
     2761 gagtcaaaaa actgggtccc tgcgatggcg tgcaggaata tttttttgct attggtacgg
     2821 agaaaaaatt cagcacccgc tggtccggca ggtgctggat gcaaacacgc cctggctcag
     2881 tcaatggcca ggcgctgcgc tgctccaccg agcttcttgg gcaaagtcaa gacgaggacg
     2941 ccgttgtcgt atttcgcttt ggctgattcc tggtcaatgt cagacggcaa ctggaagctg
     3001 cgtgagacgg caccaaaata cctttcactg cgcagcactt tctcatcctg ggtctgggaa
     3061 tcctgttgtt tgacttcggc gcgaaccgtc acaacattac cctcaaggga gacatggatg
     3121 tcctccttgt tgacccccgg cacctctgca tgcacggtat agcccttgtc agtttccttg
     3181 acatccacct tgatgcttgc ggccgtcggc agggcatcgc catgcaagga tttgacataa
     3241 aaaccaggtg ccacgtcgcg aaaaaaatcg tcaaacaggc tgccacggct gatcaatgaa
     3301 ctcatttttg cactccttct ggacttgatt taggcaccac caccacggcg ccgccatgac
     3361 accatattga ggacgcactt gacaagttca agtgcaaatt gcaccggctt tgacattcat
     3421 caacccgaca aattccggcg ggtatacctg ccgcgctata cgcccaggcg cgcaggagac
     3481 aggtattccc gcaggtacac gtcaaacggc atgctgtcgg ctgccgctat gtcctgttgt
     3541 tcctggatgg aagtagcgga ctgcgcctcc agccgggtac gtacgctgtc cggcaaaggg
     3601 acttcgagca tggcattctt tgtgccctca gactgggcgc gggcaaaggc gacgaaagaa
     3661 cctccgtact cgctttcaat cgtctgcacc acacgggcag aaggcagtgt ttcaggctgc
     3721 gccaggccaa gctttgcagc ctgcagagcc accatgtagt cggtggctcc atgtgctgta
     3781 tcaaactgca aagcaatcgg ctccatcgcc tgaaccagat cgttacccca ggctgccagc
     3841 gaaatttccg atccaccccg ctgcaactgc aaaccgggct cacgtccccg ctctgccaca
     3901 cgctgctgat tggcggcaat agctgcaatt tcagcgggcg tatcgggtgg actgtccgac
     3961 aacaggcaat acacgaggaa gacatccagg aaccgcatgg tctgggcact gatgcccagc
     4021 ggttcaaaag gattgagatc catcagacgg acttcgacgt attcaacgcc gcgctcgcgc
     4081 agcgcatgca aggggcgctc accggcgtga ataacccgct tggggcgtat cgtgccgtaa
     4141 aactcattct caatttgcag cagggacgtc gccaactgcc ggtactcgcc atccgtgccc
     4201 tgcacaccaa tggcttcgta tgccgg
//
LOCUS       DAOSLL010000431         4225 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18504_length_4225_cov_4.136930, whole genome shotgun sequence.
ACCESSION   DAOSLL010000431 DAOSLL010000000
VERSION     DAOSLL010000431.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18504_length_4225_cov_4.136930"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="PLB25_16365"
     CDS             <1..924
                     /locus_tag="PLB25_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010106132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOE43181.1"
                     /translation="KATHAGLLSRMAERVTFVLIPPLLLIFLVLGTIFLGVATPTEGG
                     AMGAMGALLMALARRRLSMSLMKQALTTTAKLSSFVVFILVGSTVFSLVFQGVDGSRW
                     VEHLLTSVPGGQMGFLIVVNILIFFLAFFLDFFELSFIVVPLLAPVAEKMGIDLIWFG
                     VLLGVNMQTSFMHPPFGFALFYLRSVAPARAYTDKLTKKLIEPVTTMQIYWGAIPFVI
                     IQIVMVGLIIAFPGIVSSGLDKAEAVDLDKVGTEMMANMPGAEPAASAPLLVPGPDAS
                     APAGEPKAPTPGAAAADDPMKAMEESMKKNK"
     gene            986..1825
                     /locus_tag="PLB25_16370"
     CDS             986..1825
                     /locus_tag="PLB25_16370"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HOE43182.1"
                     /translation="MSVPDLSLSCQYEDFLRHVNNTGVFKPDRTGTGTTSVFGYQMRF
                     DLTKGFPLVTTKKVFLRAIIVELLWFLRGDSNVKWLQERGCSIWDEWAREDGELGPVY
                     GVQWRSWPKPDGGHIDQIAEVIQTLKTNPDSRRIMVSAWNVADLDKMALMPCHAFFQF
                     YVAPPTTPGAKGRLSCQLYQRSADIFLGVPFNIASYALLTHMVAQQCDLTVGDFIWTG
                     GDCHIYSNHAAQVAQQLGRTPFAYPTLHLKRRPDSIFDYQFEDFEVLGYQCHPAIKAP
                     VAV"
     gene            1932..2819
                     /locus_tag="PLB25_16375"
     CDS             1932..2819
                     /locus_tag="PLB25_16375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOE43183.1"
                     /translation="MHSHITRRQLWGMLALTIMWGMNWPMMKLALQGMTPLFFRASTM
                     SFGAFWLFVYVARRGERMWPRDREWLTIVALGLPNVLGWHSLSIFGVQALASGRAAIL
                     GFTMPIFTVLIGWLFFAERITPRVRLAMLCAALAIGLLLWHELQRLSGRPTGVAWMLG
                     AALCWALGIVLFRRLHMTLSPMTITVWMLLLSCPVLWVLALSLEPLPQPAGFSAQLWL
                     ALAYAALINYGVSQLIWFGLARNLPPATSAMSLMAVPLVGTLSATFIVGENPHWQDWL
                     AMLFVMLTIASVLLPVRKA"
     gene            2864..3358
                     /locus_tag="PLB25_16380"
     CDS             2864..3358
                     /locus_tag="PLB25_16380"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HOE43184.1"
                     /translation="MQLKLIFARASNGIIGQNNALPWHLPEDMAHFKRCTLGCPVIMG
                     RNTWDSLPPRFRPLPGRLNLVLTRQTNWHAEGALVAHSLAQACELCPADSTAWVIGGA
                     DIYAQALPLACEAVVTEIDAAFEGDAYAPQFGPEWLEVSRQAHRSGTGLAYSFVVFQK
                     IPGV"
     gene            3360..3956
                     /locus_tag="PLB25_16385"
     CDS             3360..3956
                     /locus_tag="PLB25_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HOE43185.1"
                     /translation="MSGHGFHVHGPHDHEIEHAQQGGHGGGHGGQGSMTNQIALFTAI
                     IATIGALFGYMGGATQANAGLFKNNAAIKKTEASNQWNYFQSKSTKQSLAELARDLAP
                     EEKKASYQAKVERYEKEKTEIKAVADKLELDATAWDRQSDEQMHQHHRWAQSTTVLQV
                     SIAMAAIALLTKKKWLEYAMFVAGGVGLTVGVLAALHI"
     gene            4002..>4225
                     /locus_tag="PLB25_16390"
     CDS             4002..>4225
                     /locus_tag="PLB25_16390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOE43186.1"
                     /translation="MKLATWNVNSLTVRLPQVLDWLQANPVDVLALQELKLTDDKFPA
                     PALREAGYQAAWFGQKTYNGVAIIVKNTLP"
BASE COUNT          802 a         1265 c         1255 g          903 t
ORIGIN      
        1 aaggcaaccc acgccgggct gctgtcgcgc atggccgagc gggtgacttt tgtgctgatt
       61 ccaccgctgc tgctgatctt tctggtgctt ggcacgattt ttctgggggt tgccacgcca
      121 accgagggcg gtgccatggg tgccatgggt gccctgttga tggcactggc gcgaaggcgt
      181 ctgagcatgt cgctgatgaa acaggcgctg accaccaccg ccaagctgtc gagttttgtg
      241 gtgtttattc tggttggctc gaccgtcttc agtctggtgt ttcagggtgt cgatggttcg
      301 cgctgggtcg agcatctgct gacctcggtg cctggcggcc agatgggctt tctgatcgtc
      361 gtcaacatcc tgattttctt tttggcgttt tttctggact tcttcgagtt gtcgttcatc
      421 gtggtgccgc tgctggcacc agtggccgaa aaaatgggca ttgacctgat ctggtttggt
      481 gtgctgctgg gcgtgaacat gcaaacctcg ttcatgcacc cgccgtttgg ttttgcgctg
      541 ttttacctgc gcagtgtggc accggcgcgg gcatataccg acaagctcac caaaaagctg
      601 atcgagccag tcaccaccat gcagatttac tggggtgcga ttccatttgt catcatccag
      661 attgtcatgg tcggcttgat catcgcattc cccggcattg tctcaagcgg cctggacaag
      721 gccgaagcgg ttgatcttga caaggtggga accgaaatga tggccaacat gcccggtgcc
      781 gaaccagccg cgtccgcgcc cttgctggtg ccggggcctg acgcttcggc accagcaggc
      841 gagcccaagg cgccaacgcc tggtgcagcg gcggctgacg acccgatgaa ggcgatggaa
      901 gaatccatga agaagaacaa gtgagtcgcc aggttgctgg cagcccgaac ttcgcccggg
      961 tatctgtttg tttgaggttt cctgcatgag tgttcccgac ctgtccttaa gctgccagta
     1021 cgaggacttt ctgcgccatg tcaacaacac cggcgtgttc aagcctgacc gcactggcac
     1081 cggcaccacc agtgtgtttg gctaccagat gcggtttgat ctgaccaaag gttttcctct
     1141 ggtcaccacc aaaaaggtgt ttctgcgcgc catcattgtt gagttgctgt ggttcttgcg
     1201 cggcgactcc aacgtcaaat ggctgcagga gcgcggctgt agcatctggg acgaatgggc
     1261 tcgcgaagac ggcgaactcg gcccggtcta tggcgtgcag tggcgcagtt ggcccaaacc
     1321 cgacggcggg cacattgacc agatcgccga agtgatccag accctgaaaa ccaaccccga
     1381 ttcccgccgc atcatggtca gcgcctggaa cgtggccgac ctcgacaaaa tggcgctgat
     1441 gccatgccat gcgtttttcc agttttatgt ggccccgcca accacccccg gtgcaaaagg
     1501 ccgcttaagc tgccagcttt accagcgcag cgccgacatt tttctgggtg tgcccttcaa
     1561 catcgccagc tacgcactgc tgacccacat ggtggcgcag cagtgtgatc tgacggtggg
     1621 cgactttatc tggaccggcg gcgattgcca catctacagc aatcatgcgg cgcaggtggc
     1681 gcagcaactt ggccgtaccc ccttcgccta cccgacgctg cacctcaaac gccgacctga
     1741 ttcgatcttt gattaccagt ttgaagattt cgaggtacta gggtaccaat gccacccggc
     1801 catcaaggca ccggtggcag tctaggcgct gtgcgaccag tcaacaagct gccatcgcgc
     1861 agcgcgactg cggtgatcag cgccgccgtt tctggcgcgg ctgctgccag cgactgaaca
     1921 ccacggcaac catgcacagc cacatcaccc gccgccagct ttggggcatg ctggcactga
     1981 ccatcatgtg gggcatgaac tggccgatga tgaaactggc gctgcagggc atgacaccgt
     2041 tgttcttccg tgccagcacc atgagctttg gggcgttctg gctgtttgtc tatgtcgccc
     2101 ggcgcggtga acgcatgtgg ccgcgcgacc gcgaatggct caccatcgtg gcgctgggcc
     2161 tgcccaatgt gctgggctgg cacagcttgt caatctttgg cgtgcaggcg ctggcatccg
     2221 ggcgcgccgc cattctgggt ttcaccatgc caatttttac cgtgctgatc gggtggcttt
     2281 tttttgccga gcgcatcacc ccccgcgtgc gactggccat gctgtgtgcg gcgctggcca
     2341 ttggcctgct gttgtggcac gaactgcagc gcctgagcgg tcggcccaca ggagtcgcct
     2401 ggatgctggg tgcggcactc tgctgggcac tgggcattgt gctgtttcgc agattgcaca
     2461 tgacgctctc tccgatgacg atcaccgtct ggatgctgct gctgagctgc ccggtgctgt
     2521 gggtgctggc gctgtcgctt gagccactgc cacagccagc cgggttttcg gcccaactct
     2581 ggctggctct ggcttatgcc gcgctgatca attacggggt gtcgcagttg atctggtttg
     2641 gcttggcacg caatctgccc ccggccacca gcgccatgag cctgatggcg gtgccgctgg
     2701 taggcaccct cagcgccacc ttcatcgtcg gtgaaaaccc gcactggcaa gactggctgg
     2761 cgatgctgtt cgtcatgctg actattgcca gcgtgctgct gcctgtgcgc aaggcctgat
     2821 gccaattcaa ccccgactga caccacccat ccaaccacta ttcatgcaac tcaaactgat
     2881 ttttgcccgc gccagcaatg gcatcattgg ccagaacaac gccctgccct ggcacctgcc
     2941 ggaagatatg gcgcacttca agcgctgcac gctgggctgc ccggtcatca tgggacgcaa
     3001 cacctgggat tcgctgccgc ccagatttcg gccactgccc ggtcgcctca acttggtgtt
     3061 gacgcgccag actaactggc acgctgaagg tgccctggtc gcccattcgc tggcccaggc
     3121 ttgcgagttg tgccccgccg acagcactgc ctgggtgatt ggcggggccg acatctacgc
     3181 gcaagccctg ccgctggcct gtgaagcggt ggtgaccgag attgacgcag cctttgaagg
     3241 cgatgcttat gcgccacaat tcgggcctga gtggcttgaa gtcagccgcc aagcgcatcg
     3301 ctctggcacc ggcctggctt acagttttgt tgtttttcag aagataccag gagtttgata
     3361 tgtcaggaca tggatttcac gtacacggcc cgcacgacca cgagattgaa cacgcccagc
     3421 aaggcgggca tggtggcggc cacggcgggc aaggcagcat gaccaaccag attgcgctgt
     3481 ttaccgccat catcgccact atcggcgcgt tgtttggcta catgggcggt gccacgcagg
     3541 ccaacgccgg cctgttcaag aacaatgccg ccatcaagaa aaccgaagcc tccaaccaat
     3601 ggaattattt tcagtccaaa agcaccaaac agtcgcttgc cgaactggcc cgcgacctgg
     3661 cccctgagga gaaaaaagcc agctaccagg ccaaggtgga gcgttacgaa aaagagaaaa
     3721 ccgagatcaa ggcagtggcc gacaagcttg agcttgatgc caccgcctgg gaccgccaga
     3781 gtgacgagca aatgcaccag caccaccgct gggcccagtc caccacggtg ctgcaggtgt
     3841 cgattgccat ggcagcgatt gcactgctga ccaaaaagaa gtggctggaa tacgccatgt
     3901 ttgtcgcagg cggtgtcggg ctgactgttg gggtcctcgc agcgcttcat atttgatagc
     3961 ttgtagcgca atcaaacaaa gggctggagg gctaaatgac cattaaactg gcaacctgga
     4021 atgtcaactc gctgacggtg cgcctgccac aggtgctgga ctggttgcag gccaatccgg
     4081 ttgacgtgct ggcgctgcag gaactcaagc tgaccgatga caaatttccg gcaccagcct
     4141 tgcgcgaagc gggttaccag gccgcgtggt tcgggcaaaa gacctacaac ggtgtcgcca
     4201 tcatcgtgaa gaacacactg cccga
//
LOCUS       DAOSLL010000432         4224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18511_length_4224_cov_2.781243, whole genome shotgun sequence.
ACCESSION   DAOSLL010000432 DAOSLL010000000
VERSION     DAOSLL010000432.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18511_length_4224_cov_2.781243"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /gene="murD"
                     /locus_tag="PLB25_16395"
     CDS             <1..1152
                     /gene="murD"
                     /locus_tag="PLB25_16395"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOE43187.1"
                     /translation="VLAITGTNGKTTVTALTGQLVERAGKTVAVAGNIGPTLLDTLSQ
                     RLDTDTLPQVWVLELSSFQLDGEKDFAPTAATVLNITQDHLDWHGSMAAYVQAKTAVF
                     GSQGLMLLNREDPQVMAMLPAPVKVKLQRPQQRPFVTFGGDMPQRPGDFGLETVNGIT
                     WLVRAMDADETRRKRHEEAEELHVVRLMPADALRIRGRHNAINALASLALASAAGCAL
                     APMLYGLREYSGEPHRVQPIGLVDGIEYFDDSKGTNVGATVAALNGLGAERQVVLILG
                     GEGKGQDFSPLADPVRRHARAVVLIGRDAPLLQAALAGTGVALHDATSMGEAVQVATA
                     LAHAGDAVLMSPACASFDMFDNYEHRARVFVQAVAALAEQAGVMLEVTT"
     gene            1149..2417
                     /gene="ftsW"
                     /locus_tag="PLB25_16400"
     CDS             1149..2417
                     /gene="ftsW"
                     /locus_tag="PLB25_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HOE43188.1"
                     /translation="MSALAAGLWQRLNQWFGRFAAPADEVLPVRLGGTRLAATGVAPS
                     RAQGFDQPLVWVTVLLLLWGLVMVYSASIAMPENPRFAKYTHSYFLLRHAMWLLLAFV
                     AALLAYQVPLSTWEKSARPLFLLSLFLLALVLIPHVGKVVYGARRWISLGPLSFQPSE
                     LAKFTVLLYAADYMVRKMDVKERFFHAVWPMGAAVGVVGVLLLAEPDMGAFMVIAVIA
                     MGILFLGGVNARMFFLIAAVLLVAFAAIIASSEWRRERIFAYLDPWSEKNALAKGYQL
                     THALIAVGRGEVFGVGLGGSVEKLNWLPEAHTDFLLAVIGEEFGLLGLVLVIGLFLWM
                     VRRMMHIGRQAIAMERVFAGLVAQGVAVWMGFQAFINMGVNLGALPTKGLTLPLMSYG
                     GSAILINLVAIAVVLKIDQETRTLMHGGRV"
     gene            2414..3499
                     /gene="murG"
                     /locus_tag="PLB25_16405"
     CDS             2414..3499
                     /gene="murG"
                     /locus_tag="PLB25_16405"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HOE43189.1"
                     /translation="MSQAVKCVLVMAGGTGGHIFPGLAVAQALREQGWRVHWLGGRGS
                     ASSPSMESQLVPAQGFVFETIDFGGLRGKGLSTLALLPLRLLKAFWQSIRVLRRVNPD
                     VVVGLGGYITFPAGMMAVLLGKPLVLHEQNSVAGLANKVLAGVADRVFTAFPNVFKTA
                     RWVGNPLRSEFLNVPPPDQRFAGRNGPLQVLVVGGSLGAKALNEIVPQALALMPPAQR
                     PQVLHQSGAKQIEALRANYQAAGVSAQLTPFIDDTAQAFADADLVICRSGASTVTEIA
                     AVGAAALFVPFPFAVDDHQTSNARFLVDGQAAWLVPQAQLSPQYLAEMLSRTDRHVLM
                     SKGLEAKKMQKLDATEQVVLACEELAR"
     gene            3496..>4224
                     /locus_tag="PLB25_16410"
     CDS             3496..>4224
                     /locus_tag="PLB25_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HOE43190.1"
                     /translation="MKHAIKHIHFVGIGGVGMSGIAEVLFNLGYVVSGSDQADSATLQ
                     RLAAMGVQTFVGHAAGHVVGADAVVTSTAVQADNPEVLAARQKHIPVVPRALMLAELM
                     RLKTGIAIAGTHGKTTTTSLVASVLAEGQLDPTFVIGGRLNSAGANARLGKGDYIVVE
                     ADESDASFLNLLPVMAVVTNIDADHMETYGHDFGRLKQAFVDFLHRMPFYGTAILCTD
                     DAGVRAIEQQVTCRVTSYGFDEDAQ"
BASE COUNT          704 a         1184 c         1423 g          913 t
ORIGIN      
        1 gtgctggcca ttaccggcac caatggcaaa accaccgtga ccgcgctgac cgggcaactg
       61 gtggagcggg ctggcaaaac cgtcgcggta gcgggcaata tcggcccgac cctgcttgac
      121 accttgtcgc agcgcctcga caccgacact ttgccccaag tctgggtgct ggaactgtcg
      181 agttttcagc ttgacggcga gaaggacttt gcgccaactg ctgcgaccgt gctcaatatc
      241 acgcaagatc accttgactg gcatggttcc atggccgcct atgtccaggc gaaaactgcc
      301 gtttttggca gccagggcct gatgctgctc aaccgcgaag acccacaggt gatggcgatg
      361 ctgccagcgc cggtcaaagt caaattgcag cggccccagc agcgcccctt cgtcacgttt
      421 ggcggtgaca tgccacaacg cccaggtgac tttggtctgg aaactgtcaa cggcataacc
      481 tggctggtgc gcgccatgga tgctgacgag acccggcgca aacgccacga agaggccgag
      541 gagttgcacg ttgtgcgcct gatgccagcc gatgcgctgc gcattcgggg tcggcacaac
      601 gccatcaatg cgctggccag tctggcgctg gccagtgctg cgggttgcgc gctggcaccg
      661 atgctgtatg gcttgcgcga atacagcggc gagccgcacc gggtgcagcc cattggcctg
      721 gttgatggca ttgagtattt tgatgacagc aaaggcacca acgtcggtgc cacggtagcc
      781 gccctgaatg gcctcggtgc cgagcggcag gtggtgctga ttctgggcgg tgagggcaag
      841 gggcaagact tcagcccgct ggccgacccg gtgcgccgcc atgcgcgcgc ggtggtgctg
      901 attggccgtg atgcaccgct gctgcaggca gcgttggcgg gcaccggggt ggccttgcac
      961 gatgccacca gcatgggcga ggcggtgcag gtggcaaccg cgctggcgca cgccggtgat
     1021 gcggtgctga tgtcgccggc ctgtgccagt tttgacatgt ttgacaacta cgaacaccgc
     1081 gcccgggtgt ttgtgcaggc agtggcggcg ctggccgaac aggccggtgt gatgctggag
     1141 gtgacgacgt gagcgcgctt gcagccggcc tgtggcaacg tctgaaccaa tggtttggcc
     1201 gctttgccgc cccggctgac gaggtgttgc cggtgcggct gggcggcacc cggttggccg
     1261 ctaccggggt cgcgcccagc cgcgcccagg gttttgatca gccgctggtc tgggtcacgg
     1321 tgctgctgtt gttgtggggc ctggtgatgg tgtactcggc ctcgattgcc atgccggaga
     1381 acccgcgttt tgccaaatac acccacagct attttttgct gcgccatgcg atgtggctgc
     1441 tgctggcgtt tgtggctgcc ctgctggctt accaggtgcc cttgtcaacc tgggaaaagt
     1501 cggcgcggcc gttgtttttg ttgtcgctct ttctgctggc tttggtgttg attccccatg
     1561 tcggcaaggt ggtttatggc gcacgccgct ggatcagcct ggggcctttg agctttcagc
     1621 cgtccgaact ggccaagttc accgtgctgc tgtacgcggc tgattacatg gtgcgcaaaa
     1681 tggacgtcaa agaacggttt tttcatgccg tctggccgat gggtgcggcc gtcggggtcg
     1741 tgggcgtgct gctgctggct gagcccgaca tgggtgcctt catggtgatt gcggtgatcg
     1801 ccatgggcat cttgtttttg ggcggtgtca acgcccgcat gttcttcctg attgcggcgg
     1861 tgctgctggt ggcctttgcg gccatcatcg cgtcgagtga atggcgacgg gagcgcattt
     1921 ttgcctacct tgacccctgg agcgaaaaga atgcgctggc caagggctac cagttgacgc
     1981 atgcgctgat cgcggtcggg cgcggcgaag tttttggcgt cggcctgggt ggcagtgttg
     2041 aaaagctcaa ctggctgcct gaagcgcata ccgacttttt gctggcggtg attggcgaag
     2101 aatttggctt gctcggtctg gtgctggtga ttggcctgtt cctgtggatg gtgcgccgca
     2161 tgatgcacat cggtcgccag gcgatcgcga tggagcgtgt gtttgccgga ctggtggcgc
     2221 agggcgtggc ggtgtggatg ggttttcagg ccttcatcaa catgggcgtg aatctgggtg
     2281 ccctgcccac caagggcctc acactgcctt tgatgagtta cggtgggtcc gctattttga
     2341 tcaatctggt ggccattgct gtggtgctga agattgacca ggaaaccaga accctcatgc
     2401 acggaggccg ggtatgagcc aggctgtcaa atgcgtgctg gtgatggccg gtggcaccgg
     2461 tggccacatt tttccgggtc tggcggtggc ccaggcgctg cgcgaacagg gctggcgggt
     2521 gcattggctt ggtgggcggg gcagtgcatc gtctcccagc atggaaagcc aactggtgcc
     2581 agcgcaaggc tttgtctttg agaccatcga ctttggtggc ctgcgtggca aggggttgag
     2641 cactctggcc ttgctgcctt tgcgtttgct caaggctttc tggcaaagta ttcgggtgtt
     2701 gcgccgcgtc aatcccgacg tcgttgtggg gttgggcggc tacatcacct ttccagctgg
     2761 catgatggcc gtgttgctgg gcaagccatt ggtattgcac gagcagaact cggtggcagg
     2821 tctggccaac aaggttctgg ccggggtcgc tgaccgggtg tttaccgcgt ttccgaacgt
     2881 gttcaagact gcccgctggg tgggcaaccc gctgcgatcg gagtttctga acgtgccccc
     2941 gcctgatcag cgatttgccg gccgcaacgg cccgttgcag gtgctggtgg tggggggcag
     3001 cctgggcgcc aaagcgctca atgagatcgt gccgcaggcg ttggccctga tgccgccagc
     3061 gcagcgaccg caggtgttgc atcagagcgg tgccaagcag atcgaggccc tgcgcgccaa
     3121 ttaccaggcc gcgggtgtat ctgcacaact gaccccgttc atcgacgaca cggcgcaagc
     3181 ctttgccgat gctgacctgg tgatatgccg ttccggtgcc agcaccgtga ccgagattgc
     3241 ggccgttggt gctgcggcct tgtttgtgcc gttcccattt gccgtggatg accaccagac
     3301 ctccaacgcg cggttcctgg tggacgggca agccgcctgg ttggtgccgc aagcgcagtt
     3361 aagcccgcag tacctggccg aaatgctatc aagaacggat cgtcatgtgc ttatgagcaa
     3421 aggtctggag gccaaaaaga tgcaaaaact tgacgccacg gagcaggtgg ttctggcctg
     3481 tgaggagttg gcccgatgaa acacgccatc aaacacatac attttgtcgg cattggcggt
     3541 gttggcatgt caggcattgc agaggtgctt ttcaacctgg gctacgtcgt atcgggctct
     3601 gaccaggcgg acagcgccac attgcagcgc ctggcggcca tgggggttca aacctttgta
     3661 ggtcatgcag cgggccatgt ggtgggtgcc gacgccgtgg tgacgtcaac cgccgtgcag
     3721 gctgacaacc ccgaagtgct tgctgcgcgc cagaaacaca ttccggtcgt gccgcgcgca
     3781 ctgatgctgg ccgagctgat gcgcctgaaa accggtatcg ccattgccgg cacccatggc
     3841 aagaccacca ccaccagtct ggtggcaagt gtgctggctg aagggcagct cgaccctact
     3901 tttgtgattg gcggacgtct caacagtgcg ggcgccaacg cccgactggg caagggcgac
     3961 tacatcgtgg tggaggcgga cgagtcggat gcttcgttcc tgaacttgtt gccggtgatg
     4021 gcggtggtga ccaacatcga cgccgaccac atggaaacct atggccatga cttcggccgt
     4081 ctgaaacagg cgtttgtcga ctttttgcat cgcatgccgt tttatggaac ggccatccta
     4141 tgcaccgatg atgccggcgt acgcgcgatt gagcagcagg tcacctgccg cgtcacaagt
     4201 tatggttttg acgaagacgc gcaa
//
LOCUS       DAOSLL010000433         4220 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18533_length_4220_cov_4.234814, whole genome shotgun sequence.
ACCESSION   DAOSLL010000433 DAOSLL010000000
VERSION     DAOSLL010000433.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18533_length_4220_cov_4.234814"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PLB25_16415"
     CDS             complement(<1..310)
                     /locus_tag="PLB25_16415"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867906.1"
                     /note="Catalyzes two discrete reactions in the de novo
                     synthesis of purines: the cleavage of adenylosuccinate and
                     succinylaminoimidazole carboxamide ribotide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HOE43191.1"
                     /translation="MTTSAISALSPLDGRYAAKLAKLRPAMSEQGYMHRRVQVEVAWL
                     IALSDCGFAEFKPLSPGARTYLLSLVKNFTEADAHAIKEIEKTTNHDVKAVEYWLKGK
                     F"
     gene            378..992
                     /locus_tag="PLB25_16420"
     CDS             378..992
                     /locus_tag="PLB25_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HOE43192.1"
                     /translation="MKLIGSNTSPYVRKVRVVMAEKKLDYDYVLEDVWSADTQIGLSN
                     PLGKVPCLIMEGGEAIFDSRVIVEYFDTLSPVGRLIPTVGRERAEVKTWEALADGVLD
                     AAILARLEATWGKRTEAQRSTDWIERQLGKVDAALKAMSKGLADKPFCFGIHLSLADI
                     SVGVALGYLDFRFPQINWREQYPNLLKLQDKLMQRASFADTRPF"
     gene            complement(1005..1535)
                     /locus_tag="PLB25_16425"
     CDS             complement(1005..1535)
                     /locus_tag="PLB25_16425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOE43193.1"
                     /translation="MNTPTALHSEPPDAAAPLATRSAAAPASTGSAGDGTEPIERIVH
                     FFETLTTQSLAALGEVYDAQASFKDPFNDVTGLAAITGIFEHMFVTLHQPHFVVTGQV
                     VQGRQCFLTWEFHFGFRRFQQGQAQVIVGASHLELSDVGLITRHRDYWDAAEELYEKL
                     PLVGGLMRWLKGRARQ"
     gene            complement(1532..2311)
                     /locus_tag="PLB25_16430"
     CDS             complement(1532..2311)
                     /locus_tag="PLB25_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE43194.1"
                     /translation="MPFLNPLNPPMQQWHGKTVWIVGASSGIGLATAQAMLAAGARVV
                     VSARNAAALQALAQTHANCQALALDATDAQAVRQAANALLAQGPLDCVVYCAGHYQPM
                     RAAALDLPDMLRHCEVNYLGACYLLDAVLPALRERGQGHFSLVGSVAGYRGLPNSLAY
                     GPTKAALINLAEALYLDLHPHGLGVSIINPGFVETPLTAGNLFKMPALLTPAQAASAI
                     LQGWQQGAFDIHFPKRFTLWLKLLRLLPYRAYFSLVKQVSQ"
     gene            complement(2375..2908)
                     /locus_tag="PLB25_16435"
     CDS             complement(2375..2908)
                     /locus_tag="PLB25_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3833 domain-containing protein"
                     /protein_id="HOE43195.1"
                     /translation="MKRRLLLSAAASAAVLGGCASQNIDSYANEKPLLDLRQYFNGRL
                     DAYGVFTDRSGAVVKRFDVVMQCAWSGDEGVLDETFSYSDGSTQKRIWHLTALPGGRF
                     TGRADDVVGVAQGQARGNAFHWTYTLALPVDGRVYEVQFDDWMYLMGDGVMLNKATMR
                     KFGIYLGEVTLAFTKKI"
     gene            complement(2905..>4220)
                     /locus_tag="PLB25_16440"
     CDS             complement(2905..>4220)
                     /locus_tag="PLB25_16440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE43196.1"
                     /translation="PLYVLLPNYYARNFAVPLASLGAALLAVRLFDTLIDPLLGRWAD
                     RLHGHGQTGAAASHGTALAGRHALVLRWAGLAAVALCVGFALLFLPPVAVAGEPIRLM
                     MWLCASLLLTTIAFSALSILHQAWGARLGGDAVYRSRVVAWREGFGLAGVVVASISPV
                     ALGIQATIALLFVASWIGWQAWRSGPMPQAGSDLPMPATALSSATAQQPGAAPADRAA
                     TSTQAVSGALWLPWQRADFRRLMAVFMLNGIASAVPATLMLFFVQDRLRAPASTEPLF
                     LGSYFLCAALSLAVWLKLVARIGLARTWLAGMLLSVAVFAFATGLGAGDVAGFVVVCA
                     LSGLALGTDLALPAALLTGVIQTHGDQQRSEGAYFGWWAFASKLNLALAAGLALPLLG
                     LLGYAPGSTDAAALNTLTLAYCVLPCVLKSAAALLLYFLIIRKTP"
BASE COUNT          880 a         1293 c         1317 g          730 t
ORIGIN      
        1 tgaacttgcc tttgagccag tactcaaccg ctttcacgtc gtggttggtg gttttctcga
       61 tctccttgat ggcatgagcg tcggcttcag tgaagttctt gaccagactg agcaaatacg
      121 tgcgggcacc gggcgataac ggcttgaact cggcgaagcc acagtcgctc agggcaatca
      181 accaggccac ctcgacctgc acgcgacggt gcatgtagcc ctgctcgctc atagcggggc
      241 gcagtttggc aagtttggcg gcgtagcggc cgtccagcgg cgaaagggca gaaatggcag
      301 aagtggtcat cagcagattg tagggtgcgg tgtatagaat cacgccaaca atcaacccag
      361 cagactgtca aatgcccatc aaactcatcg gatcgaacac cagcccttac gtgcgcaaag
      421 tgcgtgttgt catggcagaa aagaagcttg attacgacta cgttctggaa gatgtttggt
      481 ctgctgacac ccagatcgga ctgagcaacc cgctgggcaa ggtgccgtgc ctgatcatgg
      541 aagggggcga ggcaattttt gattcgcggg tgatcgtcga gtactttgac acgctctcgc
      601 cggttggccg cctgataccc accgtgggtc gcgagcgcgc cgaggtcaag acctgggagg
      661 ccctggccga tggcgtgctt gatgccgcca tcctggcccg gcttgaagca acctggggca
      721 agcgcaccga ggcccagcgc agcaccgact ggattgagcg gcagttgggc aaggtggatg
      781 ctgccttgaa agccatgagc aagggtttgg ctgacaagcc gttttgtttc ggcattcacc
      841 tcagcctggc agatatttcg gtcggcgtgg cgctgggcta cctggacttc cgctttccgc
      901 agatcaactg gcgtgagcag taccccaatt tgctgaaatt gcaggacaag ctgatgcagc
      961 gcgccagctt tgccgacacc aggccgtttt gacgctaggc gcgtctactg cctggctcgg
     1021 cctttgagcc agcgcatcag gccgccgacc agcggcagct tttcatacag ctcttcggcc
     1081 gcatcccagt agtcccggtg ccgggtgatc aggccgacat ctgacaactc cagatgactg
     1141 gccccgacaa tgacttgtgc ttggccttgc tggaaacgcc tgaagccaaa gtgaaactcc
     1201 caggtcagaa aacactgcct gccttgcacc acctggccgg tcacgacaaa gtgcggctgg
     1261 tgcagggtga caaacatgtg ttcaaaaatg cctgtgatgg cggccaggcc ggtgacatcg
     1321 ttgaacgggt ccttgaagct tgcctgagcg tcataaacct cgcccagggc ggcaaggctc
     1381 tgcgttgtca gggtttcaaa aaaatggacg atgcgctcaa tcggctcggt gccatcgccc
     1441 gcgctgccgg tgctggccgg tgcagcggcg ctgcgggtgg caagcggtgc ggctgcgtca
     1501 gggggctcgc tatgcagggc tgttggggta ttcattggct gacctgtttc accaggctga
     1561 aataggcccg gtagggtaac aggcgcagca gcttgagcca aagggtgaag cgttttggaa
     1621 agtgaatgtc aaacgcgccc tgctgccagc cctgcaaaat tgcgctcgcc gcctgcgccg
     1681 gggtcagcaa ggccggcatc ttgaacaggt tgccagcggt cagcggtgtc tcgacaaagc
     1741 cggggttgat gatggacacg cccagcccgt gcggatgcaa gtcgaggtac agcgcctcgg
     1801 ccagattgat cagcgcggcc ttggttgggc cgtaagccag actgttgggc aggccacggt
     1861 aaccggcgac gctgcccacc aggctgaagt gcccctggcc acgctcgcgc aaggctggca
     1921 acacggcatc gagcaaatag caggcaccca ggtaattgac ctcgcaatgg cgcagcatgt
     1981 cgggcaaatc gagtgccgca gcgcgcatcg gctggtaatg accggcgcag tacaccacgc
     2041 agtccagcgg gccttgcgcc agcaaggcgt ttgcggcttg cctgaccgct tgcgcatcgg
     2101 ttgcatcaag cgccagcgcc tggcaattgg catgggtttg cgccaaagcc tgcagcgctg
     2161 cggcattacg ggccgacacc acgacccggg cgcctgcagc caacatggcc tgagcggtgg
     2221 ccaagccgat gccgctggag gccccgacaa tccagacggt tttgccatgc cactgctgca
     2281 tgggcgggtt caacgggttc aggaaaggca ttggattact actgtttaaa tagctggtag
     2341 cgcatgatgg ataagggctg gaggccaatt tggcttatat cttcttggta aaggccagtg
     2401 tcacttcgcc caggtagatg ccaaacttgc gcatcgtcgc cttgttgagc atcacgccat
     2461 cacccatcag gtacatccag tcgtcaaact gcacctcata aacccggccg tccaccggca
     2521 gcgccagcgt gtaggtccag tgaaaggcat tgcccctggc ctgaccctgc gccacgccca
     2581 ccacgtcgtc ggctcgacca gtgaatcggc caccgggcag ggcggtcagg tgccagatgc
     2641 gcttttgcgt gctgccgtcc gagtaagaaa aggtctcatc aagcacccct tcatcaccgc
     2701 tccaggcgca ttgcatcacc acgtcaaagc gtttcaccac ggcaccagag cggtcggtga
     2761 agacaccata ggcatcgagc cggccattga aatactggcg caagtccagc agtggttttt
     2821 cattggcgta gctgtcgatg ttttggctgg cgcagccacc cagcacggcg gcactggcgg
     2881 cggcagataa aagcaggcgt cttttcatgg ggtctttcgg atgatgagga aatacagcag
     2941 cagcgcagca gcgcttttga gcacacaggg cagcacgcaa taggccagcg tcagggtgtt
     3001 cagggcagcg gcatcggtgc tgccgggtgc gtaacccagc aaacccaaca gcggcagcgc
     3061 caggccagcg gccagcgcca ggttgagttt gctggcaaag gcccaccagc caaagtaagc
     3121 gccttcagag cgctgctggt cgccatgggt ttgaatcaca ccggtcagca gcgcggccgg
     3181 tagcgccaga tcggtgccaa gcgccaggcc cgacagcgca cacaccacca caaagcccgc
     3241 cacatcgccc gcgcccaggc cggtggcaaa ggcaaaaacc gccaccgaca gcagcatgcc
     3301 agcaagccag gtgcgcgcca gcccgatgcg cgccaccagc ttgagccaaa ccgccaggct
     3361 caaggcggcg cagagaaagt agctgcccaa aaacagcggc tcggtgctgg ccggtgcgcg
     3421 caggcggtct tgcacaaaaa acagcatcag cgtggccggc accgcgctgg caatgccgtt
     3481 gagcatgaaa accgccatca accggcgaaa gtcggcccgc tgccagggca gccaaagcgc
     3541 gccggacacc gcctgcgtgc tggtggctgc gcggtcggct ggcgcggcac caggctgctg
     3601 tgcggttgcg ctggacaggg cggtcgccgg cattggcaga tcgctcccgg cttgcggcat
     3661 cgggccactg cgccaggcct gccagccgat ccatgatgct acaaatagta gagctatggt
     3721 cgcttgtata ccaagggcta caggcgaaat tgatgcaaca accacaccgg ccagaccaaa
     3781 cccttcacgc caggccacga cccggctgcg gtagaccgca tcgcccccga gccgcgcgcc
     3841 ccaggcctgg tgcaagatgc tcagggcgct gaacgcaatg gtcgtcagca gcaggcttgc
     3901 acacagccac atcatcaggc gtatcggttc gcccgctacc gccactggcg gcaaaaagag
     3961 caaggcaaag cccacgcaca gcgccacggc tgccagacca gcccatcgca ataccagggc
     4021 gtgtcggccg gccagggcag tgccgtgact ggctgctgca ccggtttgcc catggccgtg
     4081 caggcgatcg gcccagcggc ccagcagcgg atcaatcagc gtgtcgaaca gcctgactgc
     4141 cagcagcgcc gcgcctaggc tggccagtgg cacagcaaaa ttgcgggcgt agtaattggg
     4201 cagcagcaca tacagcggca
//
LOCUS       DAOSLL010000434         4215 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18567_length_4215_cov_4.206010, whole genome shotgun sequence.
ACCESSION   DAOSLL010000434 DAOSLL010000000
VERSION     DAOSLL010000434.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4215)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4215)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4215
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18567_length_4215_cov_4.206010"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="PLB25_16445"
     CDS             complement(<1..249)
                     /locus_tag="PLB25_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine-acyl-ACP acyltransferase"
                     /protein_id="HOE43197.1"
                     /translation="MRELPNPTFALSPLSLPRGLLHRPAESVQNINQGKLLPAERGTL
                     VVSWARHQDEVRAVQRLRYRVFSDEMGARLSSNLPGHDI"
     gene            complement(277..1107)
                     /locus_tag="PLB25_16450"
     CDS             complement(277..1107)
                     /locus_tag="PLB25_16450"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HOE43198.1"
                     /translation="MHRPALLMILISVRLTRMTIHILGAFALAPVYPMLSRSAQRHIM
                     QWWSAGLLQVLNVRHNASGQPPQNASPACLLVANHISWLDIFAVNVLTPAFFVAKAEV
                     KEWAVLGWLVKRSGTLFIRRNHRSDVVRINQHMADLLHQGSPVALFPQGTSALPEQPL
                     HFHAPLLQSAVAAQAPVQPICIFYHDRQGNPHPAPAFIGDTSFIRSVWRIVGSAGIQV
                     TVGYLPPLAASGQDRRALALMAQQAVTAALAERMPARQFQRHQSATKLPQTRHQALPT
                     "
     gene            complement(1313..2845)
                     /locus_tag="PLB25_16455"
     CDS             complement(1313..2845)
                     /locus_tag="PLB25_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HOE43199.1"
                     /translation="MNPTPRTNEPQEIELKLSLPGAASAGLARCLARLPVLARHKATR
                     ESLYNIYFDTPEQTLQQQRIALRLRCIGSAASPQWRQTLKTGMSDEAALSRRGEWETA
                     VGSAALDQDALKLTPWVDIDPDGKLFEGLLPCFVTAFDRTIWRLRRPDGSVVEVALDI
                     GQLEANGRQAPICELELELKAGSSAALFELAREIAGSLAVLPANQSKAQRGFLLVQDG
                     LNRPQRAQSPRLSPNLSGPELAQRVLREMFAQFTSNLNALALSDDPEIVHQARVGWRR
                     FKSALRLLRKIAEVAAPPDRLDLQPLLAFLSELRNLDVALTETLPPLASGYCRGDEQR
                     LEIWQAMLTALTQAAALQRKAVRYALREPGVGLNLLLITEWLDALPGSNGTVTEKKVM
                     LRHWAKRRILRLHDRLQTAQKDATTQAQQHQVRILAKRLRYGVEALRDLLPKRLADFC
                     YEHAAGIQSAIGATRDLSQVSSLVASLELNPGIVEFLHGVAVGSKRASTIAQAANSLS
                     SS"
     gene            complement(2860..3609)
                     /locus_tag="PLB25_16460"
     CDS             complement(2860..3609)
                     /locus_tag="PLB25_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008452688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE43200.1"
                     /translation="MTTWILLRGLTRESRHWGRFVAHFQQALPSHRIVTLDLAGNGLL
                     NQHRSPIRVQDMVAQCRSQLSQRNIAPPYHLLAMSLGAMVAAAWALAHPQDVSAMVLV
                     NTSMRPLNPFYQRLLPANYGTLLRLILSGAAAHQWERAILRMTSNQSDESVLPHWLEL
                     RRTNPVSSLNALRQLVAAARFELPPDPPATPTLLLASEHDHLVSMQCSKSLARSWQCD
                     LCIHPSAGHDIPLDDGPWVAARVCQWLLAQR"
     gene            complement(3616..>4215)
                     /locus_tag="PLB25_16465"
     CDS             complement(3616..>4215)
                     /locus_tag="PLB25_16465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc carboxypeptidase"
                     /protein_id="HOE43201.1"
                     /translation="VGGQRLSAGLPWCRGPAGQPMEIENQAVCDIVAAELLTREFSVS
                     VDCHSGFGVRDRVWFPFAHKRAPIAHLAEMQALKSIFEQSNNHHRYLIEPQSLQYLAH
                     GDLWDHLYLQACAQSQRVFLPLTLEMGSWLWVKKNPRQLFSRHGMFNPLIEHRQQRVL
                     RQHQLLLDFLSRAACGYRLWLPTPQSRAQQHAQALQDWY"
BASE COUNT          866 a         1220 c         1306 g          823 t
ORIGIN      
        1 aatatcgtgg ccgggcaggt tgctgctgag ccgggcgccc atttcgtcag aaaaaacccg
       61 gtacctcaga cgttgcaccg cacggacttc gtcctggtgt ctggcccacg aaacaaccag
      121 cgtgccacgt tcagccggga gcaatttgcc ttgattgatg ttctgaacag attcagccgg
      181 tcggtgaagt aaccccctgg gcaatgacaa gggcgacagc gcaaacgtgg gattgggtaa
      241 ctctctcatt tctctctcct tggattaagt gccgctctat gttggcagcg cttgatgacg
      301 tgtttgcggc agttttgtgg cgctttgatg acgctgaaac tggcgcgcag gcatccgttc
      361 ggccagcgct gccgtcactg cctgttgcgc catgagagcc agcgcacggc ggtcttgtcc
      421 agatgccgcc agcggcggga gataacccac ggtgacctgg ataccggcgc tgccgacaat
      481 tcgccagacc gacctgatga agctggtgtc accaatgaat gctggtgccg gatgtggatt
      541 gccctgccgg tcatggtaaa aaatgcagat gggttgaacc ggtgcttgcg cagcaacggc
      601 gctttgcagc agcggcgcgt gaaagtgcag tggctgctct ggcaatgccg aggttccttg
      661 cggaaacagc gcaaccggac tgccctgatg cagcagatca gccatgtgct gattgatgcg
      721 gaccacatcg cttctgtggt tgcgccggat aaacaaggtg cctgagcgct ttaccagcca
      781 gcccagcacg gcccattcct tgacttcggc tttggcaacg aagaaagccg gggtgagcac
      841 attgacggca aaaatgtcca gccacgatat gtggttggcc accagcaagc aagccggaga
      901 agcattttgt ggcggttggc cgctggcgtt gtggcgcaca ttcaagacct gcaacaagcc
      961 ggcagaccac cattgcatga tgtgccgctg agcggaccga ctcagcatcg ggtaaaccgg
     1021 ggcgagcgca aatgcgccaa gaatgtgaat ggtcatgcgg gtcagccgaa cgctgatcag
     1081 gatcatcaaa agagcagggc gatgcatggt tttccaaaaa aagacgggac gacaggtgcg
     1141 gtcaagtcca gcgccacaac cagcagcatg gcgaccgcga atgacttcaa aaaagaggat
     1201 gctgccggtt tcggtcaaac cgatgaacct gcagccgaat tcgacaacgc cttgcgccgc
     1261 agggcatcga agacacaagg gaaagcgcca tcaatcttga gtctggcgtg cgtcaacttg
     1321 atgacaagct gttggccgct tgcgcaatgg tgctggccct ttttgagcca actgccacac
     1381 catgcaaaaa ctcgacaatg ccgggattca attcgagtga tgcgaccagt gaactgactt
     1441 gagagaggtc cctggtggct ccgatggccg attgaattcc cgctgcatgc tcatagcaaa
     1501 aatcagccag tcgtttgggc aagaggtcac gcagcgcctc aaccccatag cgcaaacgct
     1561 tggcaaggat gcgcacctga tgctgctggg cctgagtcgt ggcatctttc tgtgccgttt
     1621 gcaggcgatc atgcaaacgc agaatccggc gcttggccca atgacgcagc atgacttttt
     1681 tctccgtcac agtaccgttt gagccgggta gcgcgtccag ccactctgtg atcaacagca
     1741 aattcaagcc aacacctggt tctcgcagcg catagcgcac cgccttgcgt tgcagtgcgg
     1801 cagcctgggt caacgccgtc agcatggcct gccagatctc aagccgctgt tcatcccccc
     1861 ggcaatagcc gctggccaaa ggtggcaagg tttcggtcag ggccacatca aggttgcgca
     1921 gttcgctcag aaaagccagc agcggttgca aatcaagcct gtccggtggt gctgccactt
     1981 ccgcgatctt cctgagcagg cgcaaggccg acttgaagcg ccgccagcca acgcgcgcct
     2041 ggtgaacgat ctcggggtca tctgacaggg caagtgcgtt gaggttggag gtgaattggg
     2101 cgaacatttc gcgcagcaca cgctgggcca gttccggtcc ggacaggttt gggctcagcc
     2161 tgggggattg ggcacgttgc ggccggttga ggccgtcctg caccagcaaa aagccgcgct
     2221 gcgccttgct ctggttggct ggcaacaccg ccaggctgcc agcgatctcc cgggccagct
     2281 caaacagcgc agcagatgag cccgctttca gttcaagctc aagttcgcaa atgggtgcct
     2341 gccggccatt ggcttccaat tgaccgatgt ccagcgcaac ttcgaccacg ctgccgtctg
     2401 gcctgcgcaa gcgccaaatg gtccggtcaa aggcagttac aaagcatggc aacaaacctt
     2461 caaacagctt gccatccggg tcgatgtcga cccacggcgt gagcttgagc gcatcctgat
     2521 ccagcgcagc actgccaacg gctgtctccc attcgccgcg gcgactcagg gcagcttcgt
     2581 cactcatgcc ggtcttcagg gtttgccgcc actgcggact cgcggcactg ccgatgcagc
     2641 gcagacgcaa ggcaatgcgt tgctgctgca gcgtctgttc tggcgtgtcg aaatagatgt
     2701 tgtagagcga ctctcgcgtt gccttgtgcc gggcaagcac tggcaggcgg gccaggcatc
     2761 tggccaaacc ggccgatgct gcacctggca ggctcagctt gagctcaatc tcttgaggct
     2821 cgttggtgcg gggtgttggg ttcatgggtg ttttgacctt tatcgttgtg ccagcaacca
     2881 ttggcacacc cgcgctgcga cccaggggcc atcatcgagg ggaatgtcat gaccggcgct
     2941 ggggtgaatg cacaaatcgc attgccagct tctggccagt gacttgctgc attgcatcga
     3001 gaccaaatga tcgtgttcac tggccagcag cagggtgggg gtggcgggtg ggtcaggcgg
     3061 tagctcaaag cgtgccgccg ccaccagttg gcgcagcgca ttcaggcttg acacggggtt
     3121 ggtgcgccgc agttccagcc agtgcggcaa caccgattca tcagactggt tgctggtcat
     3181 gcgcaggatg gcgcgctccc actggtgcgc cgctgcgccc gacagtatca agcgcaacag
     3241 cgtgccatag ttggccggta gcaggcgctg gtaaaacggg ttcaaggggc gcatgctggt
     3301 attgaccagc accatggcgg aaacgtcttg tggatgcgca agtgcccagg cggcggccac
     3361 catggcgcca agtgacatgg ccaacagatg gtagggcggc gcaatgttgc gttggctgag
     3421 ctggctgcgg cactgcgcca ccatgtcctg cacccgaatc gggctgcggt gctgattgag
     3481 caaaccattg ccggccaaat ccagcgtgac gatccggtga cttggcaaag cctgctgaaa
     3541 atgagcgaca aagcgccccc aatgtcggct ttcccgggtc agtccacgca gcaatatcca
     3601 ggtggtcatg gtcagtcaat accagtcttg cagggcttgg gcgtgctgct gcgcgcggct
     3661 ttgcggcgtt ggcagccaaa gccggtagcc acaggctgcg cgtgacaaaa agtccagcag
     3721 caactggtgt tggcgcagca cccgctgctg gcggtgttcg atcaacgggt tgaacatgcc
     3781 gtggcgtgaa aagagctgac gcgggttttt cttgacccac agccaggagc ccatctcaag
     3841 cgtgagtggc agaaacaccc gctgcgactg tgcgcaggct tgcaggtaca aatggtccca
     3901 aaggtcgccg tgcgccaggt attgcaggct ctgcggctcg atcaggtaac ggtgatggtt
     3961 gttggactgc tcaaaaatgc ttttcagcgc ctgcatctcg gccaggtggg cgatgggtgc
     4021 gcgtttgtgc gcaaagggaa accagacccg gtccctgaca ccgaagccgg aatggcaatc
     4081 caccgagacg ctgaattcgc gtgtcagcag ttccgctgcc acgatatcgc aaaccgcctg
     4141 gttttcgatc tccatcggct ggcccgctgg acctcggcac cagggcaggc cggcgctgag
     4201 gcgctggccg ccgac
//
LOCUS       DAOSLL010000435         4211 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18586_length_4211_cov_4.187199, whole genome shotgun sequence.
ACCESSION   DAOSLL010000435 DAOSLL010000000
VERSION     DAOSLL010000435.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4211)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18586_length_4211_cov_4.187199"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..551
                     /locus_tag="PLB25_16470"
     CDS             <1..551
                     /locus_tag="PLB25_16470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43202.1"
                     /translation="AETGITLKEDKSAIEKAEDYLNGQSLDEAAINIRKAAEATVARF
                     RTGTVVATEDFVGLTKQLNSAKRKLLERIPFQLYEQVLKDLPQSIRQHLVPRTDVGLS
                     DVAGITTEQKAEIVRLRLKLNDVVSNMHWQMMENAKIVDEVLATTARVLNPGSHGGET
                     PLYDSEVRQALELIQRLERCLS"
     gene            649..1599
                     /locus_tag="PLB25_16475"
     CDS             649..1599
                     /locus_tag="PLB25_16475"
                     /inference="COORDINATES: protein motif:HMM:NF014548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HOE43203.1"
                     /translation="MTKPPNTTEPANHIAVFQETTIRRTWHNKEWWFALSDVIIVLTD
                     SADAKQYIKKMRSRDPALDANWGTTCTPLAILAPDGKMRETNCANTEGLFRIIQSIPS
                     PKAEPFKRWLAQVGYERVKEIENPELASARARELYQAKGYPQAWIEKRLRSISVRGEL
                     TDEWKARGVAEGKEYSILTAEIARATFGVTPSEHSQLKSLDQVKTGNNLRDHMTDLEL
                     IFTMLGEASTTEIARRADAQGFVDNCKSAKQGGTIAGNARKALEAKTGNKVVSKSNCL
                     TVPATSDLLGEPQTVAAQRIKPKPRAQVKLPAKPTRGAAE"
     gene            complement(1868..3142)
                     /gene="urtA"
                     /locus_tag="PLB25_16480"
     CDS             complement(1868..3142)
                     /gene="urtA"
                     /locus_tag="PLB25_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HOE43204.1"
                     /translation="MQSLNSRRFTLKALTAAVALSTLSALPALAAETIKVGILHSLSG
                     TMAISETVLKDTVLMAIDEINAKGGVLGKKLEPVVVDPASNWPLFAEKTKQLLGQDKV
                     AVIFGCWTSVSRKSVLPVVEEMNGLLFYPVQYEGEELSKNVFYTGAAPNQQAIPAVDY
                     LMSKDGGAAKRWVLLGTDYVYPRTTNKILRAYLKSKGVKDTDIDEKYTPFGHSDYQTI
                     VADIKKFSAGGKTAVVSTINGDSNVPFYKELGNAGLKAKDVPVVAFSVGEEELRGVDT
                     KPLVGHLAAWNYFQSIKNPTNDEFIKKWTAYAKAKGIAGHKDKALTNDPMEATYIGIN
                     MWKQAVEKAKSTDVDKVIAAMAGQTFKAPSGIVSKMDEKNHHLHKSVFIGEIKADGQF
                     NVVWKTPGPVKAKPWSPYIEGNDKKPDEPAKK"
     gene            3370..3930
                     /gene="greB"
                     /locus_tag="PLB25_16485"
     CDS             3370..3930
                     /gene="greB"
                     /locus_tag="PLB25_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HOE43205.1"
                     /translation="MSKAFTKEPDDADDDEALVPPLPASGKNYMTPAGYARLRAELLE
                     LIDVERPKIVDVVHWAASNGDRSENGDYLYGKKRLREIDRRIRFLTKRLEIAEVVNPS
                     VHFGNVQIFFGATVTYLETDGIARTITIMGIDEAVSANGEVSWVSPIARTLLKARQGD
                     VLNLATPAGPLEIEVVKVSYPASLTT"
     gene            complement(3906..>4211)
                     /locus_tag="PLB25_16490"
     CDS             complement(3906..>4211)
                     /locus_tag="PLB25_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOE43206.1"
                     /translation="GVEWADEPVRAFETGIVVTVSNGKGVLARVAGALSAAEADITQV
                     DLADESRQGALDIRFVIAVRDLTQLDTVFSNLRRAPAVLKVQRSKNAAPRQVVSDAG"
BASE COUNT          953 a         1181 c         1182 g          895 t
ORIGIN      
        1 acgctgaaac cggaatcacg ttgaaagaag ataagtcggc gattgaaaaa gcggaggact
       61 atctgaatgg gcagagcctc gacgaagctg caatcaacat tcggaaagct gctgaagcca
      121 cggtagcgcg atttcgcact ggtaccgtcg tcgccacgga agattttgtt ggactcacca
      181 aacaactgaa ctctgccaaa aggaagctgc tggagcggat tccctttcaa ctgtatgagc
      241 aggtactaaa agacctaccg cagtctataa ggcagcatct tgtgcccaga acagatgtag
      301 ggctgtcgga tgtggctgga atcacgactg agcaaaaagc agaaatcgtg agactacggt
      361 tgaagctgaa tgatgtcgtg tccaacatgc actggcaaat gatggaaaac gcgaaaattg
      421 tggatgaagt acttgcaacc accgcgcgtg tactcaaccc tgggtcacat ggtggcgaga
      481 ctcccctgta tgactcagag gttcgccaag cgctcgaact gatccaaagg ctggagaggt
      541 gcctttcgta aagccgcagg gttgagtatc catgtcaaat tagcctgaaa cgctcgctta
      601 ataagcgtaa gaagctacaa aagttatacc aaacatgagg agactatctt gacaaaacca
      661 cccaatacta ctgagcccgc caaccacatc gccgtttttc aggaaaccac catccggcgt
      721 acctggcaca acaaggagtg gtggtttgcc ttgagtgatg tcatcatcgt actgaccgat
      781 tcggctgatg ccaagcaata catcaagaag atgcgcagcc gtgacccggc gctcgatgcc
      841 aactggggta caacttgtac cccccttgcg atcctggctc cggatggaaa aatgcgcgaa
      901 accaactgcg ccaacaccga aggcctgttc cgcatcatcc agtccatccc gtcgcccaaa
      961 gccgaaccct tcaagcgctg gttggcgcag gtgggctacg agcgcgtaaa agaaatcgaa
     1021 aaccccgaac tggccagcgc ccgtgctcgt gagttgtacc aagccaaagg ctacccgcag
     1081 gcgtggattg aaaaacgcct gcgctccatc agcgtgcgcg gtgagttaac cgatgagtgg
     1141 aaggcacgcg gcgtggccga gggtaaagag tattcaatcc tgaccgccga gatcgcccgg
     1201 gccacctttg gcgtgacccc cagcgaacac agccagctca aaagcctcga ccaagtcaaa
     1261 accggcaata acctgcgaga ccacatgacc gatctggagc tgatcttcac catgcttggc
     1321 gaagccagca ctaccgaaat tgcccgccgc gccgatgcgc agggctttgt tgacaactgc
     1381 aaatctgcca aacagggcgg caccattgcc ggtaacgccc gcaaagcgct ggaagccaaa
     1441 acgggcaata aggtggtgag caaatccaat tgcctgacgg tgcctgccac atcagatttg
     1501 cttggcgaac cacaaactgt ggccgcccag cgcatcaaac ccaagccccg cgcccaagtg
     1561 aaattgcctg caaagcccac ccgtggtgcg gcggagtgac agcaccagtc ttggcatcct
     1621 gacagacacc accggtacaa cattggaaat gacggtacag aaagtcttac cgcagcgctg
     1681 gcacttcgcc cgcttgttga cttcccatcg gttaccagcc gctgatcaat gcagtccgac
     1741 gtgccgctgt tgacccggac gcggcacccg acaaaaagcc cgcacggggc gggctgttac
     1801 aagttggcaa agagtcattg acccgacaga ccgttagcca gcctgtgcgc catggtggcc
     1861 gtcggtgtta cttcttggcg ggctcgtcag gcttcttgtc gttgccttcg atgtacggtg
     1921 accatggctt ggctttgacc gggccggggg ttttccagac cacgttgaac tggccatctg
     1981 ccttgatttc gccgatgaac actgacttgt gcaggtggtg gtttttctcg tccatcttgc
     2041 tgacgatgcc gctcggtgcc ttgaaggtct ggccagccat ggcggcgatg accttgtcaa
     2101 cgtcggtgga cttggccttt tcaaccgcct gcttccacat gttgatgcca atgtaggtgg
     2161 cttccatcgg gtcgttggtc aatgccttgt ctttgtgacc ggcaatgccc ttggctttgg
     2221 cgtaggcagt ccacttcttg atgaattcgt cgttggtcgg gttcttgatc gactggaaat
     2281 agttccaggc agccaggtgg ccgaccagcg gtttggtgtc aacaccgcgc aactcttctt
     2341 ctccaacgct gaatgccacc accggcacgt ctttggcttt caggccagcg ttgccaagtt
     2401 ccttgtaaaa cggcacgttc gagtcaccgt tgatggtgga caccacggcg gtcttgccac
     2461 cagcagagaa cttcttgatg tcagccacaa tggtctggta gtccgaatgg ccaaacggcg
     2521 tgtacttttc gtcaatgtcg gtgtctttca cgcccttgga tttgaggtag gcgcgcaaaa
     2581 tcttgttggt ggtgcgcggg tacacgtagt cggtgcccag cagcacccag cgcttggcag
     2641 cgccgccgtc cttgctcatc aaatagtcaa ctgccggaat cgcttgttga ttcggtgcgg
     2701 caccggtgta gaacacgttc ttggacaact cttcgccttc gtactgcacc gggtagaaca
     2761 gcaggccgtt catttcttca accaccggca gcacagactt gcgcgacaca ctggtccagc
     2821 agccaaaaat cacagcaacc ttgtcttgtc ccagcaactg cttggtcttt tcggcaaaca
     2881 gcggccaatt ggaagcgggg tcaaccacca cgggttcgag cttcttgcca agcacgccgc
     2941 ccttggcgtt gatttcgtca atcgccatca gcacggtgtc tttcaacacg gtttccgaaa
     3001 tggccatggt gccagacaag ctgtgcaaaa ttccgacctt gatggtttcg gcagccagcg
     3061 ccggcagggc cgacagggtg gaaagtgcga ccgcagcggt gagcgctttt aacgtgaaac
     3121 gacgtgaatt caaagattgc atgtgggcta ctccaaagtt gatgaaaccg ttgtcgccgg
     3181 gcaacttttg ccggttaggg cgttgctcga cgatgatgca actgtaggca ggcgcgccgg
     3241 tttgggaaat acgccaagtg gcgtatgagg ccggaggcag cggccatgct gtgccgcctg
     3301 gtccagtgtt gctcattgct tgcctggcat cacgctatga tcaaagttgc ctgttgcgat
     3361 tgaggtttta tgagcaaagc gtttaccaaa gagccagacg atgcggatga cgacgaggcg
     3421 ctggtgccgc cattgccagc cagtggcaag aactacatga cgcctgcggg ctacgccaga
     3481 ctgcgtgccg agttgctgga actgatcgat gtggagcgac ccaaaattgt cgatgttgtt
     3541 cactgggccg caagcaacgg tgatcggtct gaaaacggcg attatttgta cggtaaaaag
     3601 cgcctgcgtg aaattgaccg ccgcatccgg tttctgacca agcgtctgga gattgccgag
     3661 gtggtcaacc cttcggtgca ctttggcaac gtacaaattt tctttggtgc cacggtgact
     3721 tacctcgaaa ccgacggcat tgcgcgcacc atcacgatca tgggcattga cgaggccgtc
     3781 agcgcaaacg gcgaggtgag ttgggtgtcg ccaattgccc gcaccttgct caaggcaaga
     3841 cagggtgatg tgctgaattt ggcgacgccg gctggcccac ttgagattga agtggttaag
     3901 gtgtcttacc cggcatcgct cacgacttga cggggtgccg cgttttttga tctttgaacc
     3961 ttgagcacgg caggggctcg ccgcaagttg ctgaaaactg tgtccaactg ggtcaggtca
     4021 cgcactgcga tgacaaagcg gatatccagc gctccttgcc gtgattcgtc ggccagatca
     4081 acctgggtga tgtcggcctc tgcagcagag agcgcgccag ccaccctggc cagcacccct
     4141 ttgccgttgc tgactgtgac cacaatgccg gtctcgaagg cgcgcaccgg ctcgtcagcc
     4201 cattcaaccc c
//
LOCUS       DAOSLL010000436         4182 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18761_length_4182_cov_4.430579, whole genome shotgun sequence.
ACCESSION   DAOSLL010000436 DAOSLL010000000
VERSION     DAOSLL010000436.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4182)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18761_length_4182_cov_4.430579"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(300..1094)
                     /gene="fabI"
                     /locus_tag="PLB25_16495"
     CDS             complement(300..1094)
                     /gene="fabI"
                     /locus_tag="PLB25_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HOE43207.1"
                     /translation="MGFLTGKKLLITGVLSNRSIAYGIAKACHAQGAELAFSYVGERF
                     KDRITEFAADFNSKLIFDCDVGDDAQIDKLFADLALHWPKFDGFVHSIGFAPREAIAG
                     NFLDGLSRENFRIAHDISAYSFPAMAKAALPYLNNTASLLTLTYLGGVRVVPSYNTMG
                     LAKASLESSVRYLADAVGGRGIRVNGLSAGAIKTLAASGIKDFGKLLGISAAASPLKR
                     NVTIDEVGNVAAFLLSDLASGMTGQISYVDCGVSQTAVAVVETPGS"
     gene            1383..2180
                     /locus_tag="PLB25_16500"
     CDS             1383..2180
                     /locus_tag="PLB25_16500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HOE43208.1"
                     /translation="MRIFVFSAGLLMFFSSLSVQAQLATPASVPFASVAQAAAVLPRI
                     ESAENPYGIESLWKTSDAVARIVLLLLLAMSVSSWYVTVVKLLDQSKMARQARAAADD
                     FWSAGTIEEGARALEGNSPYRFIADAGTNALRKHDGLMGHIPLNEWVFMSIQRAVDRI
                     QSNTQQGLAVLATVGSIAPFVGLFGTVWGIYHALTAIGISGQASIDKVAGPVGEALIM
                     TAMGLAVAVPAVLAYNWLVRRNKAVMDDVRAFGQDLHAVILGTVSKS"
     gene            2426..2857
                     /locus_tag="PLB25_16505"
     CDS             2426..2857
                     /locus_tag="PLB25_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOE43209.1"
                     /translation="MAMRIGSDDDAPDGLMSVINTTPLVDVMLVLLIIFLITIPVVTT
                     SIPVALPKQRTAVRESKPDNVIISVDRSGAIFYYDARIRSVDALVEKLKQVAVARPQP
                     EVQVRGDMGAQYEGIGKVLLACQRAGIVKVAFITEPPPLGG"
     gene            2927..3304
                     /locus_tag="PLB25_16510"
     CDS             2927..3304
                     /locus_tag="PLB25_16510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010095493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HOE43210.1"
                     /translation="MDINTTPLIDVMLVLLVMLIITIPIQLHSVALELPVAARATQLI
                     KVENIRIDIDEHNVVHWQGVSVTAVELDDKMTAVAQQNPQPEVHIRPDKRSRYAIFAG
                     VLASSRRTGLSKIAVIGAEQFVP"
     gene            3301..3993
                     /locus_tag="PLB25_16515"
     CDS             3301..3993
                     /locus_tag="PLB25_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HOE43211.1"
                     /translation="MIFSNLNDHYLQSDPSRRINGVVIVLLVHALIGYALICGTARQS
                     LQLIKKPQASVVIQEVTIAPPPAKPPPPPPAQKVLRPAQPQVAEVPAQPRFMPMPDAP
                     VALGTVASPAAMAAEPAAPVPTAPPSPSPSPSPSPAPTAAVAKPSRNDMDVACPIQVS
                     PEMPPRALREGAQGVVRAKALIRDGAVKEVTIVSGPPVFHAAVKAAMLQYKCTHDAQE
                     VTVFQQFNFKFE"
BASE COUNT          851 a         1144 c         1284 g          903 t
ORIGIN      
        1 ggggtcgcgc tggcggggcg gtggggtggt ggggtggtgg ggtggtggcg cgatgtcgct
       61 tgcggctcac ctggcacggc cagaactgag cctgcttgac cgaaccatgt tgatcatgtt
      121 gacctgcgtg gcgcgtggat tgccatcgcc atgcggtctg cgatgtcggc cagatcaagc
      181 gccagatcaa gcgccagatc aagcgccaga tcaagcgata ccgccatcct gcaaacctga
      241 tgagctacac caataatagc tgtcagcgct tgctacacaa aggctacagc ccaatttggt
      301 tatgaacccg gagtttccac cacggccacc gccgtctggc tgacaccgca atcgacatac
      361 gaaatctgtc cggtcatgcc cgatgccagg tcactcagca agaatgcggc cacgttgcca
      421 acctcgtcaa tcgtcacatt gcgcttgagc ggcgacgcag ccgccgaaat gcccagcagc
      481 ttgccaaagt ccttgatgcc gctggccgcc agcgtcttga tggcaccggc gctcaggccg
      541 ttgacacgga tgccgcggcc accgactgcg tcggcgaggt aacgcaccga ggactccagc
      601 gatgccttgg ccaggcccat ggtgttgtag ctcggcacca cacgcacgcc acccaggtag
      661 gtcaaagtca acagcgaggc agtgttgttc aggtagggca gggccgcttt ggccatggcc
      721 ggaaagctgt aggcgctgat gtcgtgggcg atgcggaagt tctcgcgact caaaccgtcg
      781 agaaaattgc cggcaatcgc ctcccgtggg gcgaaaccga tgctgtgcac aaaaccgtcg
      841 aatttgggcc agtgcagcgc caggtcggca aacagcttgt cgatttgggc atcgtcgcca
      901 acatcgcaat caaaaatcag ctttgagttg aaatcggcgg caaattcggt gatgcggtcc
      961 ttgaaacgct cgcccacata gctgaacgcc agctcggcac cctgtgcatg gcatgctttg
     1021 gcgatgccat aggcgatgga gcggttcgac aacacgccgg tgatgagtaa ttttttgcct
     1081 gtcaggaatc ccatatcgac cttttcaatt tttctggctg ctggtaaacc actgccttaa
     1141 ccccgaagga tgaaaccgca gtgacagatg atgcagaatt gtcgcatgtg ttctctgtgg
     1201 catggctacg cagcgcattg ctgcggcatc ggcagatttg gcgcggcgat tttggggaca
     1261 atcggtgcag ctaaagcggc ctcatcggcg tgaatcaagg gtgttgcctg ccgaagaagt
     1321 tgcgccactg gtggtcggga aatttcgaaa gggtttttgt gcgagttgtt tgtgttgggt
     1381 ggatgcgtat ttttgtcttt agcgcaggct tgttgatgtt tttttcttcc ctttcggttc
     1441 aggcccaatt ggccacccca gccagcgtac cgtttgcgtc tgtggcacag gctgctgctg
     1501 tgctgccccg gattgaatcg gctgaaaacc cgtacgggat cgaatcgctg tggaagacct
     1561 ctgacgcagt ggcgaggatc gtacttttac tgctgctggc gatgagtgtg agcagttggt
     1621 atgtcaccgt tgtcaaactg ctggatcagt ccaaaatggc gcggcaagcc agggctgctg
     1681 ccgatgattt ctggtctgcg ggcaccatcg aagagggggc acgtgctctt gaaggcaaca
     1741 gcccgtatcg ctttattgcg gatgccggca ccaacgcttt gcgaaagcac gacggcttga
     1801 tgggtcatat tccgctgaac gaatgggttt tcatgagtat ccagcgtgcc gtggaccgga
     1861 ttcagagcaa cacccagcaa ggtctggcgg tgctggccac ggtgggttcc attgcgccat
     1921 ttgtcgggct gtttggaacc gtctggggca tttaccatgc gctcacggcc attggcattt
     1981 ctggccaggc ctcgatcgac aaggttgccg gcccggttgg cgaggccttg atcatgaccg
     2041 ccatgggact ggcggtcgct gtgccggcgg tgctggcgta taactggctg gtgcgccgca
     2101 acaaagccgt catggacgat gtgcgggctt ttggccagga tctgcatgcc gtcattctgg
     2161 gaaccgtcag caagagctga cttgatcggc tggcttggca catgcgtgaa tggcagattt
     2221 cgccgggagc caggcgcact gatggatcga gcagcgcgca ccgaccccag cgtggtcagt
     2281 ttgcgccagc gtttcagacc catggcccag ttctgggaca aaaaaaggcg ggtgccaatt
     2341 gggccgctgc ggtcaattcg cagtcgatgc gcgctgaatc cgccgggctg cttgcagcca
     2401 tttggcactt attcgggata ttgacatggc gatgaggatt ggatcagatg acgatgcgcc
     2461 ggacggcctg atgtcggtga tcaatacgac accgctggtg gacgtgatgc tggtgttgct
     2521 gatcattttc ttgatcacca ttccggtcgt gacgacctcg atacccgtgg ctttgcccaa
     2581 acagcgtacc gcggtgcgtg aaagcaagcc ggacaacgtc atcatttcgg ttgaccgcag
     2641 cggtgccatt ttttactacg atgcgcgtat cagatcggtg gatgcgctgg ttgaaaagct
     2701 caagcaggtc gctgttgcca ggccacagcc tgaagttcag gttcgcggcg atatgggtgc
     2761 gcaatacgag ggtatcggca aggtgctgct ggcctgccag cgtgccggca ttgtcaaagt
     2821 ggcttttatt accgagccgc cgcctttggg gggttaactg gcaatgacca acttgatggc
     2881 aagaaagctg ggacattcgg atggctctgg cgaaccggaa ttgatgatgg acatcaatac
     2941 cacgccgctg attgacgtga tgctggtctt gttggtgatg ctgatcatca ccattccgat
     3001 ccagttgcat tcagttgcgc tggaactgcc ggtggcagcg cgcgccaccc agttgatcaa
     3061 ggtggaaaac atccgcattg acattgacga acacaatgtc gtccattggc aaggcgtgtc
     3121 ggtcaccgcg gtcgaactcg atgacaagat gacggctgtc gcgcagcaaa atccccagcc
     3181 cgaggtgcat attcggcccg acaagcgttc acgctatgcg atttttgccg gtgtgcttgc
     3241 aagcagtcgg cgcaccggtc tctccaaaat agcggtgatc ggtgccgagc agttcgtccc
     3301 atgattttca gcaacttgaa tgaccactac ttgcagagtg atccgtcacg ccgcatcaac
     3361 ggggttgtga ttgtgctgct ggtgcatgcg ctgattggct atgccctgat atgcggcacg
     3421 gcaagacaga gtttgcagct catcaaaaag ccccaggcaa gcgttgtgat tcaggaagtg
     3481 acgattgcgc cgccaccagc aaagccaccg ccgccaccgc cagcgcaaaa agtgctcaga
     3541 ccggcccagc cccaggtggc cgaagtgcct gcgcagcccc gtttcatgcc aatgccggat
     3601 gccccggtgg cactgggcac ggtagccagc ccggctgcaa tggcagcgga acccgcagcg
     3661 ccagtgccga ccgctccgcc atcgccatcg ccatcgccat cgccatcgcc agcgccgacg
     3721 gcagccgtgg ccaaacccag ccgaaatgac atggatgtgg cttgtccgat ccaggtgtct
     3781 ccggagatgc cgcccagggc gctacgggag ggcgcgcaag gcgtggtccg ggcgaaggcg
     3841 ctgatcaggg atggcgcagt caaagaggtg accatcgtgt cggggccgcc agtgttccac
     3901 gcagcggtga aagctgccat gctgcaatac aaatgcaccc atgacgcaca ggaggtcacg
     3961 gtgtttcagc agttcaattt caagtttgag tagcttgatg catgcatgtt ggcgcaagcg
     4021 aacctgttca aatgcattcg agcataaagc atggactttt tcgacgcatg aaattgagtc
     4081 acaccacttg gctgaaaggg tttgagattg tttgaccttg acggactgaa tcaatgccat
     4141 aagtctgatc ggcgactcgc tgtctggagc ctgttgctgc tg
//
LOCUS       DAOSLL010000437         4180 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18768_length_4180_cov_4.156364, whole genome shotgun sequence.
ACCESSION   DAOSLL010000437 DAOSLL010000000
VERSION     DAOSLL010000437.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4180)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4180
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18768_length_4180_cov_4.156364"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..1308)
                     /locus_tag="PLB25_16520"
     CDS             complement(91..1308)
                     /locus_tag="PLB25_16520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HOE43212.1"
                     /translation="MELIEVSTDKLFLGMSLEYALRDASGLVLLAKGQKIESSQQLAA
                     IIGRQHVFIDIDQTGSGVRAMMSSLSALELAGAPIKDFSRYIEIKKSGPAEVRKIGTL
                     SQRWGDVESNLGGLLASLETTDDFANRIGGLEQFIAQLLAEDENGSQFLLFNRAVTHF
                     EGYSVIHGLLCAALTSSLVQVFALTDAQRCSLVCAALTMNVGMTRLQDLLAVQKHAPN
                     QHQRSVIDAHASIGRKLLEKAGVTDQTWLAVVAQHHAPINGPEGLLTWPAAKRMTKIL
                     QTVDRYTAAMSPRKSRSGRTARDSVRAVVIQTGEAKHDEVGTALMRVLGLSPPGTFVK
                     LANGETAVVMRRGVKPAEPWVASVLNRNDEPIAEPHLRDTSRAGFAISSTLTASSVRV
                     NLNIEMMLRLIPK"
     gene            1460..2677
                     /gene="dapC"
                     /locus_tag="PLB25_16525"
     CDS             1460..2677
                     /gene="dapC"
                     /locus_tag="PLB25_16525"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019877 - diaminopimelate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HOE43213.1"
                     /translation="MNSLLANLQPYPFERLRQLLSGIRPNPAYNPVSLGLGEPKHATP
                     AIIQQAMVDAVKRTPSGLCSYPATAGELSLRNAFAAWLKRRYGLDLDPATEMLPILGS
                     REALFALTQTVVDRTAGIDGQKPLVVSPNPFYQIYEGSALLAGAEPYFVACDSLQNFA
                     PNWDAVPEAAWARTQLLFVCSPGNPTGAVMPLSEWQKLFDLSDRFGFVIASDECYSEI
                     YFRDEPPLGGLEAAAKLGRSNYKNLISLTSLSKRSSVPGMRSGFVAGDANIIRQFLLY
                     RTYHGSAMSGVVQAASTVAWSDEVHVVENRALYRAKFPHVMPLLADVLGVALPDAAFY
                     LWADVAGDEIAFTRDLYAAYNVTVLPGSYLAREAQGHNPGQGRIRLALVAETAECVEA
                     ASRIRDFVISRAA"
     gene            2728..3552
                     /gene="dapD"
                     /locus_tag="PLB25_16530"
     CDS             2728..3552
                     /gene="dapD"
                     /locus_tag="PLB25_16530"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HOE43214.1"
                     /translation="MTQQLQTILDAAWEDRANLSSASVSKEVSDAVEHVIHQLNNGQL
                     RVATRQAVGQWTTHQWIKKAVLLSFRLKDNEIMRAGDLGFYDKVPTKFSQMDSPGMSA
                     TGVRVVPPAVARRGCFLAKGVVLMPSFVNIGAYVDENSMVDTWAAVGSCAQIGKNVHL
                     SGGVGIGGVLEPMQANPTIIEDNCFIGARSEVVEGVIVEENSVISMGVYIGQSTPIYD
                     RASDSVSYGRIPAGSVVISGSLPKEGGKYGLYAAIIVKRVDAQTRAKTSLNDLLRP"
     gene            3765..>4180
                     /locus_tag="PLB25_16535"
     CDS             3765..>4180
                     /locus_tag="PLB25_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili twitching motility protein PilT"
                     /protein_id="HOE43215.1"
                     /translation="MAAIDNAQHGTMRRILRLMADKRASDVYLSANSPATIRINGVCV
                     PINSQVLPPEAPLSLLSEILPPERIEELQDLNELNMGWSMPGVGRFRISGMRQRGSYA
                     AVIRFIVNEIPPLASLMLPPTLADLMLQKRGLILMVG"
BASE COUNT          878 a         1271 c         1160 g          871 t
ORIGIN      
        1 actcagcagc cagggtgctg gtgtgccaca gccgtgtggt agcgagcttc ggtgcgaaac
       61 agaaactggc agcaacacgc caagtaagtg ttacttgggg atcagccgca gcatcatttc
      121 aatgttcaga ttgacccgca ccgacgacgc tgtcaatgtg gaagatatcg caaaacccgc
      181 acgcgaggtg tcacgcaaat ggggctcggc gatcggttca tcgttgcgat tcagcacgct
      241 cgccacccag ggctcggcgg gtttgacacc gcgtcgcatc accaccgcag tttcaccgtt
      301 ggccagcttg acaaaggtgc ctggcggact cagccccagc acgcgcatca gcgcggtgcc
      361 tacctcatcg tgcttggcct cacccgtctg gatcacgacg gcgcgcaccg agtcacgcgc
      421 ggtacgcccc gaacgtgact tgcgcgggct catcgcggcc gtgtagcgat caaccgtttg
      481 caggattttt gtcatgcgtt tagctgctgg ccaagtcaac aaaccctccg gcccgttgat
      541 cggcgcgtgg tgctgcgcca ccaccgccag ccaggtctgg tctgtcacgc ctgccttttc
      601 cagcaatttg cgaccaatgc tggcatgggc atcgatcacg ctgcgctggt gctgattggg
      661 ggcgtgcttt tgcacggcaa gcaagtcctg caagcgtgtc atgccaacat tcatggtcaa
      721 tgctgcacaa accaggctgc agcgttgggc atcggtcagc gcaaacacct ggaccagcga
      781 acttgtcagt gcggcgcaca acaggccgtg gatcacgctg taaccttcaa aatgtgtcac
      841 tgcacggtta aacagtaaga actgcgatcc gttttcgtcc tcagccaaca actgggcaat
      901 gaactgttcc agtcctccaa tacggttggc aaaatcgtct gtcgtttcca gacttgccag
      961 caagccaccc aggtttgact ccacatcacc ccaacgctgt gacagagtgc cgatcttcct
     1021 gacctcggcc ggaccgctct ttttgatttc gatatatctg gaaaagtcct tgatcggtgc
     1081 gccagcgagc tcaagcgccg aaaggctcga catcatggcc cgcacaccgc tgccggtctg
     1141 gtcaatgtca ataaaaacat gctgccggcc gatgatggca gcaagttgct ggcttgactc
     1201 gattttttgc cccttggcaa gcaaaaccaa accgctggca tcacgcaagg cgtattccag
     1261 cgacatgccc aaaaagagtt tatcggttga aacttcgatc agttccatgg ccagccaaag
     1321 acaagaccat gcagcagcca aacatcagtc ggcggtgctg cagacgaggt gttgggcaaa
     1381 cgcaagacat cagacatatc gcgccgagtt tagctgccag cttcggcttt gttcaagacc
     1441 tccttgataa tcagccgcca tgaactccct gctcgcaaac ctgcagccct acccgtttga
     1501 gcggttgcgt caacttcttt ccggcattcg gcccaaccct gcatacaacc ctgtcagtct
     1561 ggggctgggt gaacccaagc acgccacacc agcgatcatc cagcaagcca tggtggacgc
     1621 agtcaaacgc acacccagcg gcttatgctc ctacccggca accgccggtg agctgtctct
     1681 gcgcaacgcc tttgccgcct ggctgaaacg ccgctatggc ctggaccttg accctgccac
     1741 cgaaatgctg ccaattttgg gctcgcgcga agccttgttt gccctgacac aaaccgtggt
     1801 tgatcgcact gctggcatcg acgggcaaaa gccactggtg gtcagcccga acccgttcta
     1861 tcaaatctac gagggctcag ccctgctggc gggggccgaa ccctatttcg tcgcttgcga
     1921 ctccttgcag aatttcgccc cgaactggga cgctgtgcct gaagcagcct gggcccgcac
     1981 ccaactgctg tttgtctgct cacccggcaa cccgactggt gccgtgatgc ccctgtcgga
     2041 atggcaaaaa ctgtttgact tgagcgaccg ttttggcttt gtgatcgcat cggacgagtg
     2101 ttacagcgaa atctattttc gcgatgaacc cccgttgggc ggcctggagg cggcggccaa
     2161 actcggccgc agcaactaca aaaacctgat ttcgctcacc agcctgtcca aacgcagcag
     2221 cgtgcctggc atgcgcagcg gctttgtggc gggtgatgcc aacatcatca ggcaattttt
     2281 gctctatcgc acctaccacg gcagcgccat gagcggtgtg gtgcaggccg ccagcacagt
     2341 ggcctggtct gatgaggtgc acgtggtgga aaaccgcgcc ctgtaccgcg ccaagtttcc
     2401 ccacgtcatg ccactgcttg ccgacgtgtt gggcgtggcc ctgcccgatg ccgcctttta
     2461 cctatgggct gacgtggcag gcgacgagat tgccttcacg cgcgatcttt acgctgctta
     2521 caatgtcacc gttttgccag gctcctacct ggcccgcgaa gcgcagggcc acaaccccgg
     2581 ccaaggccga atccgcttgg cgctggtggc tgaaaccgcc gaatgtgtgg aagctgccag
     2641 tcgcatcagg gactttgtca tcagccgcgc cgcttgacgg tctgcgccaa tcagaccttt
     2701 ttttaaatct ctttcgaata cagccaaatg acccaacaac tgcaaaccat ccttgatgcc
     2761 gcgtgggaag accgcgccaa cctgtcaagc gcatcagtct ccaaagaggt ctccgatgcg
     2821 gtggaacatg tgattcacca actgaacaac ggtcaactgc gggtcgccac tcgccaagca
     2881 gtgggtcaat ggaccaccca tcaatggatc aaaaaggccg ttctgctgag ctttcggctg
     2941 aaagacaacg agatcatgcg tgctggggac ctgggctttt acgacaaggt gccgaccaag
     3001 ttctcgcaaa tggacagccc gggcatgagt gccaccggcg tgcgcgtggt gccaccagcg
     3061 gtggcccggc gcggctgctt tctggccaaa ggcgtggtgc tgatgccatc ctttgtcaac
     3121 attggcgcat acgttgatga aaacagcatg gtggacacct gggctgcggt gggttcttgt
     3181 gcccagattg gcaaaaatgt gcatttgagc gggggtgtcg gcattggcgg cgtgctcgag
     3241 ccaatgcagg ccaacccgac aatcattgaa gacaattgtt ttattggtgc ccgctccgag
     3301 gtggttgaag gggtcatcgt tgaagaaaac tcggtgatct ccatgggagt ctatattggc
     3361 caaagcaccc cgatttacga ccgagcctct gacagcgtca gttatggccg cattcccgct
     3421 ggctcggtcg tcatcagcgg cagcttgccc aaagagggag gcaagtacgg cctgtatgcc
     3481 gccatcatcg tgaaacgagt tgacgcgcaa acccgcgcca aaaccagttt gaatgatctc
     3541 ctgagacctt gagggacaga ccaggagtta cacgcagtga acgatgctct gtccccaact
     3601 gattttgggc tgttccccct tgagggacag accaggagtt acttgcgcag ccaagctgcg
     3661 gggcagtgaa caatgctctg tccccaaccg atcctggacg gttgccttga gttgtgccag
     3721 gcgaagatca ctgctttatc ctcaattgat tagaaagccc ccctatggcc gccattgaca
     3781 atgcccagca cggcaccatg aggcgcattt tgcggctgat ggccgataag cgcgcctccg
     3841 atgtctacct gtcagcaaac tcgcctgcga cgattcgcat caacggggtg tgcgtaccca
     3901 ttaacagcca ggtgttgccg cccgaagcac cgctgagcct gctcagtgaa attctgccgc
     3961 ccgaacgcat cgaagaactg caagacctca acgagttgaa catgggctgg tcgatgccgg
     4021 gggtcgggcg ctttcgcatc agtggcatgc gccagcgcgg ctcgtatgct gcggtgatcc
     4081 gtttcattgt caacgagatc ccgccgctgg catccttgat gctgcccccc acactggccg
     4141 atctgatgct gcaaaagcgc ggtctgatcc tgatggtcgg
//
LOCUS       DAOSLL010000438         4179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18774_length_4179_cov_4.851115, whole genome shotgun sequence.
ACCESSION   DAOSLL010000438 DAOSLL010000000
VERSION     DAOSLL010000438.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18774_length_4179_cov_4.851115"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..972)
                     /locus_tag="PLB25_16540"
     CDS             complement(40..972)
                     /locus_tag="PLB25_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HOE43216.1"
                     /translation="MTSAHDHRHHHTDGHADANFNHAFAIGIALNTAFVAIEAFYGWR
                     INSLALLADALHNLSDVAGLVLAWGAALAGKLRPDARHTYGFKRASILAAFCNAALLL
                     LAMGSLGWEALGRLQAPEPTQGWTIIAVAGVGIVINAATALLFMRGAHGDLNIRGAFL
                     HMVGDALVSLAVVVGGALYLWQGWSWIDPLLSLAIAVMIVLGTWGLFRQSLHLLFDGV
                     PAHIDLAAVRHLLLTLPGTADVHDLHVWAMGTSDVALTAHLVLAEAVTDSSAVLHQAE
                     HELHEHFEIRHVTLQLESTEHARHCALGSAAGCH"
     gene            complement(1008..2468)
                     /locus_tag="PLB25_16545"
     CDS             complement(1008..2468)
                     /locus_tag="PLB25_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE43217.1"
                     /translation="MHLTSRLGSCLRLRLHLPELPSSLYARLALILLVGLLAAQGISL
                     WLQWGERSAVVTQARGLNFADRLAHAVSVLETVGLAQRGAALAALQYDDLSVTLVNAN
                     QVSARVPRGPIADLLAARLGSQRQVRHVPAPSGAHAGAGNGAGGGAGGAAMGTMQGDA
                     VQRSFDVQLQDGQWARVTIRRNTESNAPALSNDLILQLLLTLVIVAAVVMIAVRQATR
                     PLQQLAQAADRLGHDLDAAPLVPGGSTETRRAAQAFNRMQTRIQHLIRERARALAAVS
                     HDLRTPLTRLRLRTELIDDAQLRDQMASDLASMAAMLDTTLDYLRGLQAHELVRPIDI
                     NALLESMAEDALVPGRKIRIDGLAQKPFDGRLTALRRALQNLIDNAFKFGHAVCLQVQ
                     DDALRLSITVQDDGPGIAIAELGKVVEPYYRVDSARSQAGDGVGLGLSIVRDMALMHG
                     GELLLANRPQGGLAATIILPREQPLAALAEPVRKAV"
     gene            complement(2459..3190)
                     /locus_tag="PLB25_16550"
     CDS             complement(2459..3190)
                     /locus_tag="PLB25_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005663395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE43218.1"
                     /translation="MEKTDCILIVDDEPEIRRLLVDYLVRNGFEALAARDGRDMWQVL
                     ERHAVDLLVLDLMLPDTDGLTLCRDLRARSNLPVLMLSARGEDADRIVGIEMGADDYL
                     VKPFNPRELLARIKSILRRTRALPPNLRPEPVRCLRFAGWCLDTAERLLTAPDGVATP
                     LSGGEFRLLRILLEHPNRVVTREQLIEMIHGREAEAYDRAIDVQVSRLRHRLRDDSRE
                     PQLIKTVRGQGYVLAASVEPGNTCI"
     gene            3356..3745
                     /locus_tag="PLB25_16555"
     CDS             3356..3745
                     /locus_tag="PLB25_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43219.1"
                     /translation="MKTSYAFKTIAIATTLAGFLSASVLAQSAPVAAPGTGAGPGAGQ
                     MGPGAGGMRGMQFGQNNTPGWTLMTAEERTAHQTSMRAVKTYDECKLLQAEQHATMQA
                     RAKEKGITLNTPRQNGCDNMKARGFIQ"
BASE COUNT          854 a         1336 c         1267 g          722 t
ORIGIN      
        1 tatgcagcaa gcgctaccag ctacattata aatagcaatc taatggcagc cggcggcact
       61 gcccagcgca cagtgtcggg catgctcagt tgattccagt tgcagtgtga cgtggcgaat
      121 ttcaaaatgc tcgtgcaact catgttcggc ctggtgcagt acggcactgc tgtcggtaac
      181 tgcctcagcc agcaccagat gcgccgtcaa ggcaacgtcg gaggttccca tggcccagac
      241 gtgcaggtca tgcacgtcgg ccgtgccggg caaggtcagc agcaaatggc gcaccgctgc
      301 caggtcaata tgggccggta cgccgtcaaa cagcaggtgc aatgactgcc tgaacaaacc
      361 ccaggtgccc agcacgatca tcaccgcaat ggccaggctc agcagcgggt cgatccagct
      421 ccagccctgc caaaggtaaa gtgcaccgcc caccaccacc gccagcgaca ccagggcatc
      481 gccgaccatg tgcaaaaaag ccccgcgaat gttcaggtcg ccgtgcgcgc cacgcatgaa
      541 cagcagcgcg gtggccgcgt tgatgacgat gcccacgcca gccactgcaa tgatggtcca
      601 gccctgcgtg ggttcgggtg cctgcagtcg cccgagcgcc tcccagccaa gtgagcccat
      661 ggccagcagc aacagtgcgg cattgcagaa agccgccaga atcgaggcgc gcttgaaacc
      721 gtaggtgtgg cgggcatccg ggcgcagctt tcctgccagc gctgcgcccc aggccagcac
      781 cagtccggcg acatcgctca ggttgtgcag cgcgtcggcc aacagcgcca gcgaattgat
      841 gcgccagcca taaaacgcct cgatcgcgac aaatgcggtg ttcaaggcga tgccaatggc
      901 aaaggcgtgg ttgaagttgg catcggcatg gccatcggtg tgatggtgcc ggtgatcatg
      961 ggcgctggtc atgctgcaca gcccggttta acaatggttt gcaagggtca cactgccttt
     1021 cgcacaggtt ctgccagcgc ggccaaaggt tgctcgcgtg gcaagatgat tgtcgctgcc
     1081 agcccgccct gcggccgatt ggccagcaat aactctccgc cgtgcatcag ggccatgtcg
     1141 cgcacgatcg acagccctaa cccaaccccg tcaccagcct ggctgcgtgc gctgtccacg
     1201 cggtaatacg gctcaacgac tttgccaagt tcggcaatgg caatgccagg accatcgtct
     1261 tgcaccgtga tgctcaggcg cagggcgtcg tcttgcacct gaaggcagac ggcgtgaccg
     1321 aatttgaaag cgttgtcgat caggttttgc agcgcccgcc gcaaggcggt caagcgaccg
     1381 tcaaaaggct tttgggccag tccgtcaatg cggatctttc gccccggcac cagcgcgtct
     1441 tctgccatgc tctccagcag ggcgttgatg tcaattggac ggaccagttc gtgcgcttgc
     1501 agtccacgca gatagtccag ggtggtgtca agcatggccg ccatggatgc caggtcgctg
     1561 gccatctggt cgcgcaactg tgcatcgtca atcagctcgg tgcgcaggcg caggcgtgtc
     1621 aggggtgtgc gcagatcgtg cgacaccgcc gccagcgcgc gtgcgcgttc ccggatcagg
     1681 tgctggatac gggtttgcat gcggttaaag gcttgcgctg cgcggcgggt ttcggtgctg
     1741 ccgcctggca ccagtggcgc ggcatccaga tcatgaccaa gcctgtcggc cgcctgcgcg
     1801 agctgctgca gtggacgggt ggcttgcctg acagcgatca tcaccacggc ggcaacgatc
     1861 accagggtca gcagcagttg caggatcagg tcgtttgaca gtgccggggc atttgattcg
     1921 gtgttgcgcc ggatggtgac gcgggcccat tggccatctt gcaactgcac atcgaaactg
     1981 cgctgcaccg catcgccctg catggtgccc atggcagccc caccagcccc accgcccgcc
     2041 ccattaccag ccccagcatg ggcaccagac ggggctggaa catgccgaac ctggcgctgg
     2101 ctgccgagtc gggccgccag caggtcggca atcggcccac gcggcaccct ggctgacacc
     2161 tggtttgcgt taaccagcgt gacgctcagg tcgtcgtatt gcagggccgc caacgccgcg
     2221 ccacgttgcg ccaatcccac cgtctccagc acgctcaccg cgtgggccag gcggtcggca
     2281 aagttcagcc cacgggcctg cgtgaccacc gccgagcgct cgccccattg caaccacagg
     2341 ctgatgcctt gcgcggcgag caggccgacc agcaaaatca gtgccaggcg ggcgtacaag
     2401 ctcgacggca gttcaggcag gtgcagacgc agtcgaaggc aggagcccaa gcgagatgtc
     2461 aaatgcatgt gttgcctggc tcgacactgg cggccagcac ataaccttgg ccgcgcacgg
     2521 tcttgatcaa ctggggttcg cggctgtcat cgcgcaagcg atggcgcagg cgactgacct
     2581 gaacgtcaat ggcgcggtcg taggcttcgg cttcgcggcc atgaatcatc tcgatcagtt
     2641 gctcacgcgt cacgacccgg ttggggtgct ccagcaagat gcgcagcagg cggaattcgc
     2701 cacctgacag cggtgtggca acaccatcgg gtgccgtcag cagccgctcg gcggtgtcga
     2761 gacaccaacc cgcaaagcgc aggcagcgta ccggctcagg ccgcaaattc ggtggcaatg
     2821 cgcgggtgcg acgcaagatc gatttgatgc gggccagcaa ttcacgcggg ttaaagggct
     2881 tgaccaggta atcgtcggca cccatctcga tgccgacaat gcggtcggca tcttcacccc
     2941 gcgcgctcag catgagcact ggcaggttag acctggcgcg caggtctcga cacagcgtaa
     3001 ggccatcggt atcaggcagc atcaggtcca gcaccagcaa gtcaacggcg tggcgctcca
     3061 gcacctgcca catgtcacgc ccgtcgcgtg ccgccagcgc ttcaaaacca ttgcgcacca
     3121 gatagtccac cagcaagcgg cgaatttcgg gctcgtcatc gacgatcagg atgcagtcag
     3181 ttttttccat acgtgctcag tttgtaaccc actgtaacgc aaggccacag cgcaacatgg
     3241 cgatgcaaat cagcgcttta cagcaacgct gcgttgacac agacgcgctc aaatagcgct
     3301 cagggatgca ggcatgacca gcagcccaca tcggttcaat cacaggagtt ccaccatgaa
     3361 aacctcttac gctttcaaaa ccatcgccat tgccaccaca ctggctggtt tcttgtctgc
     3421 atcagtactg gcacaaagcg ccccggtcgc ggcccccggc accggtgcag gccctggtgc
     3481 cggccagatg ggacctggcg caggtggcat gcgcggcatg cagtttggcc agaacaacac
     3541 acctggctgg accttgatga cagccgagga gcgcaccgct caccagacca gcatgcgcgc
     3601 agtcaaaacc tatgacgaat gcaagctgct gcaagccgag caacatgcga ccatgcaagc
     3661 acgtgccaag gagaaaggca ttaccttgaa taccccgcgc cagaatggtt gtgacaacat
     3721 gaaagcgcgt ggttttatcc aatagcttct gcctgcacca aaggcaacgc gcctggcggc
     3781 gttgctgttc ctggtcgcca gtagcctgtg tcgccttcgc ccttcgcggc ctgctgcagc
     3841 atgcagggcg caggcctgcc acaaggctgg cctgcttcca gcctgaacag gtagcttggc
     3901 agccgtggcg ttcgccctgt ccaaccgtgc cgccagcggc ccactcgggt gcgtccaact
     3961 gaagaagttg ggttgcagcc ggtgaatgtg gcaaaacgac acataagccg cggaaaaaac
     4021 accgaatcgg cctgcaagcc atatagcaag ggcgcagaca gctactgata gtgtagcaca
     4081 tcggttgcga catacgccgc atgcaggtgt tattgtgggc gcatcggcac aacaacggct
     4141 ttgcgcgcag ccggtccagg ccgccacccc tgtgccagg
//
LOCUS       DAOSLL010000439         4177 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18787_length_4177_cov_5.371179, whole genome shotgun sequence.
ACCESSION   DAOSLL010000439 DAOSLL010000000
VERSION     DAOSLL010000439.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18787_length_4177_cov_5.371179"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..779)
                     /locus_tag="PLB25_16560"
     CDS             complement(117..779)
                     /locus_tag="PLB25_16560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HOE43220.1"
                     /translation="MKLHNVEQARFNMIEQQIRPWDVADAAVLQLLSVVRREDFVPVG
                     YKAMAFADMAIPLAAGQSMLAPRLQARLLQDAAVRSGDKVLEIGTGSGFMTALLAHQA
                     QRVVSLEINAELADQARANLQKAGVHNAEVRQADGSVSTCAEAPFDVIVLGGSVAEVP
                     ATLLAQLKVGGRLVGIVGDEPIMHAQVITRTSDSNFKSDEKWDDNAPRLLNFPQPPAF
                     QF"
     gene            complement(850..4032)
                     /locus_tag="PLB25_16565"
     CDS             complement(850..4032)
                     /locus_tag="PLB25_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOE43221.1"
                     /translation="MWFTRVSLNNPVFATMVMLALVVLGLFSMQRLQVDQFPNVDFPV
                     VVISTEYPGASPEIVESEVTKKLEEGVNSIAGINALTSRSYESQSVVIIEFGLHIDGR
                     KAADDVREKVASVRPNLRTEVKEPRVLRFDPSSRAIWSVAVLPDDKAAGAAAMSAVEL
                     TNWAEQTLKKRLENVRGVGSVTLVGGSKREINIYLNPQALESFGITPDQVASAVRNEN
                     QDFPVGSIRSAEQDRVIQIAARVQRPEDFGQIIITRKNGAAVRVNQVAQVSDGAQELD
                     SLALYNGQRTLLLNIQKSQDENTIAVVDGLNKTLAELQEALPAGVKLAQIADGSRQIR
                     VSVANVRQTLFEGALLTVLIVFLFLNSWRSTVITGLTLPIALIGTFLFMNLLGFTVNM
                     ITMMALSLCVGLLIDDAIVVRENIVRHVQMGKDAFTASMDGTREIGLAVFATTLSIVA
                     VFLPIGFMGGIIGKFFHEFGLTIVAAVLISMFVSFTLDPMLSSIWHDPSIEIDGQRHQ
                     PVNFYDKTIGRVTALFDRGTEALAEIYQGILRWALQHKLATVALAMGIFVGSVFMVPL
                     LGTEFVPKSDFSETSLTFNTPVGSSLEVTEARARQVEGIIRSFPEVRYTLTTINTGNA
                     QGKIYANIYIRLVERKARARNVDAMSGVLRQRLREVPGITVTHVGLLDPVGGQKQISF
                     SIQGADMGELSRLTELALEKIRPVAGLVDIDSTLKPNKPTLDVQVRRDAASDQGLSVA
                     QIGSALRTLVAGQTVGNWRAPDDQTYDVNVRLSPQSRQQPGDLNALPFVLGSSADGSA
                     RVVRLNQVASVVEATGSNQINRRDLTREVAISGNVAGRSAGEISADIKLAMDSIAMPA
                     GYSYKFSGSTKDMAEAFAYALSALLLAVVFIYMILASQFKSFFQPLALMTALPLTLIG
                     VVLALLMFNSTLSMFSVIGVVMLMGLVTKNAILLVDFTIRARAPALDAHGQSVPGSPR
                     HEALLMAARVRLRPILMTTLAMIFGMMPLALALSEGSEMRAPMGQAVIGGVVTSSLLT
                     LVVVPVVYCYMDDLAQWLRRLVHRPSSSPR"
BASE COUNT          873 a         1279 c         1199 g          826 t
ORIGIN      
        1 gcttgatgcc cgcagctcat cgggttcacg gacatccagc acgatcgggt tgccatgctc
       61 acgcaccgat gcaaaccagt tggccaattg ggcaggtcgg acttgggtca gcatgttcag
      121 aactgaaagg cggggggttg cggaaaattg agcaggcgcg gcgcgttgtc gtcccacttc
      181 tcatcggact tgaaattgct gtcgctggta cgggtgatga cctgggcatg catgatgggt
      241 tcatcgccca caatccccac cagacgacca ccgactttga gttgggccag caaagtggcc
      301 ggcacttctg ccaccgaccc ccccagcaca atgacatcaa atggtgcttc tgcgcaagtg
      361 ctaaccgagc cgtcggcctg gcgcacctcg gcgttgtgga caccagcctt ttgcaggttg
      421 gcgcgcgcct ggtcagccaa ttcagcgttg atctccagcg agactacacg ttgtgcctgg
      481 tgcgccagca aagccgtcat gaagcctgag ccagtgccaa tttccagcac cttgtcgcca
      541 gatcgaacgg ccgcgtcttg caacaatcgg gcttgcagac gaggtgccag catgctttga
      601 cctgcggcca gcggaatggc catgtcagca aaagccatgg ccttgtagcc gacgggcaca
      661 aaatcttcgc gcctgaccac agacaacagt tgcagcacgg cagcgtcggc cacgtcccag
      721 gggcgaatct gttgttcgat catgttgaag cgggcctgtt cgacgttgtg caatttcatt
      781 taattactcc tgggggtatg ttagcggaat cgtcctattt tagctggcag cactgtcatt
      841 ggcctgaatt tagcgggggc ttgacgacgg tctgtgcacc aacctgcgca gccactgggc
      901 cagatcgtcc atataacagt acaccaccgg caccacgacc agcgtcagca acgacgaggt
      961 gacgaccccc ccaatcaccg cctgccccat cggggcgcgc atctcagagc cctcggatag
     1021 cgcaagtgcc agtggcatca tgccaaaaat catcgccagc gtggtcatca agatggggcg
     1081 caggcgcacc cgggcagcca tcagcaatgc ctcatggcgt ggcgaacctg gcacagactg
     1141 gccgtgtgca tccagcgccg gggcacgcgc ccgaatggtg aagtccacca gcaatatcgc
     1201 gtttttggtg accagcccca tcagcatcac gacaccgatc accgagaaca tcgacagagt
     1261 cgagttgaac atcagcaacg ccagcaccac gccaatcaat gtcagcggca gtgcggtcat
     1321 tagcgccagc ggctggaaaa aactcttgaa ctggctggcc aatatcatgt agatgaacac
     1381 taccgccagc agcagggccg acagtgcata ggcaaaggct tcagccatgt ccttggtcga
     1441 gccactgaat ttgtagctgt atcctgcggg catggcaatg ctgtccatcg caagtttgat
     1501 gtcagccgat atttcaccgg cagaccggcc agcaacattg ccgctgatgg ccacttcgcg
     1561 cgtcaggtcg cggcggttga tctggttgga tccggtggct tccaccacgc tggcgacctg
     1621 gttcagccgc accacccggg cgctgccatc ggcgctgctg cccagcacaa acggcagtgc
     1681 attgaggtca ccgggctgtt gccgcgactg gggtgacagc cgcacgttga catcgtaggt
     1741 ctgatcatcc ggtgcgcgcc agttgcccac cgtttgccca gctaccaggg tacgcagggc
     1801 cgatccaatc tgtgccacgc tcaggccctg gtctgaggcc gcatcgcggc gcacttgcac
     1861 gtcaagcgtc ggcttgttgg gtttgagggt cgaatcgatg tccaccaaac ccgccaccgg
     1921 cctgattttt tccagtgcca gttcggtcaa tctggacagc tcacccatgt cagcgccctg
     1981 aatggagaag gaaatctgct tttgcccacc gaccgggtca agcaggccga catgggtcac
     2041 ggtgatgccc ggcacttcgc gcagccgctg gcgcaacacc ccggacatgg catccacatt
     2101 gcgcgctcgc gccttgcgct ccaccagccg gatgtaaatg ttggcgtaaa ttttgccctg
     2161 agcgttgcca gtgttgatgg tggtgagcgt gtagcgcact tcgggaaacg agcggatgat
     2221 gccttcgacc tggcgcgccc gggcctcggt cacttccagc gatgatccga ccggggtatt
     2281 gaacgtcaag ctggtttcag aaaagtcgga tttgggcaca aattcggtac ccagcagcgg
     2341 caccatgaag acactgccaa caaaaatgcc cattgccaga gcgactgtgg ccaatttgtg
     2401 ctgcagtgcc cagcgcaaga taccttggta aatttccgcc agcgcttcgg tgccccggtc
     2461 aaacagggca gtgacgcggc caattgtctt gtcataaaag ttgaccggct ggtgccgctg
     2521 gccatcgatc tcgatgctgg ggtcatgcca gatacttgac agcatcgggt caagggtaaa
     2581 gctgacaaac atcgagatca gcaccgctgc cacgatcgtc aagccaaact catggaaaaa
     2641 cttgccgatg atgccgccca tgaaaccaat cggcaaaaat actgccacga tcgaaagggt
     2701 ggtggcaaac actgccagcc caatttcccg cgtgccgtcc attgacgcag tgaacgcgtc
     2761 tttgcccatt tgcacatggc gcacgatgtt ctcccgcacc acaatcgcat catcaatcag
     2821 caggccgaca cacaggctca gcgccatcat ggtgatcatg ttgacggtaa aaccaagcag
     2881 gttcatgaac agaaaagtgc caatcagggc aatcggcagg gtcaggccgg tgatcaccgt
     2941 ggagcgccac gagttcagga acaaaaacac aatcagcacc gtcagcaggg caccttcaaa
     3001 cagggtttgc cgcacgttgg ccaccgacac ccggatttgc cgcgagccgt cggcgatctg
     3061 cgccagtttg acccccgctg gcaaggcttc ttgcagttcg gccaatgtct tgttcaggcc
     3121 atcgaccacc gcaatggtgt tttcgtcttg cgacttctgt atgttaagca gcagtgtgcg
     3181 ctggccgttg tagagcgcca gactgtcaag ctcctgcgcg ccatcgctga cttgagcgac
     3241 ctggttgacc cgcaccgctg caccattttt gcgggtgatg atgatctggc caaagtcttc
     3301 cgggcgctgc acccgggcgg caatctggat cacccggtct tgctcggcag agcggatgct
     3361 gccgaccgga aagtcctgat tttcgttgcg taccgcactg gcgacctggt ccggtgtgat
     3421 gccaaaagac tccagcgcct gcgggttgag gtaaatattt atttcgcgct tgctgccacc
     3481 gaccagcgtg accgacccga ccccgcgcac gttctccaac cgctttttca gggtttgctc
     3541 ggcccagttg gtcagttcga cggcactcat cgccgccgcc ccagccgcct tgtcatctgg
     3601 caacacggcc accgaccaga tggcccggct ggatggatcg aaacgcagca cccgtggctc
     3661 tttgacctcg gtgcgcaaat tggggcgcac cgaggccact ttttcacgca cgtcgtcggc
     3721 ggctttgcgt ccatcgatat gcaggccaaa ttcgatgatc acaaccgact ggctttcata
     3781 cgagcgcgaa gtcagcgcgt tgatgccagc gatcgaattg acaccttctt caagcttctt
     3841 ggtgacttcg ctttcgacga tttcgggcga agcgccgggg tattcggtgc tgatcaccac
     3901 caccggaaag tcaacattgg ggaactggtc tacttgcagc cgctgcattg agaacaggcc
     3961 cagcaccacc agcgccagca tcaccatggt ggcgaaaacc gggttattca ggctgacacg
     4021 ggtgaaccac attcagccta gattccccag ttggacgcgc ccgagtgggc cgctggcggc
     4081 gcggctgggt aggcgcgacg acgcagcggg ctcctgcccg ctaggaggag caacgacccc
     4141 aggcgtgttg ccagtggctc aatcgggtgc gtagcgc
//
LOCUS       DAOSLL010000440         4172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18816_length_4172_cov_3.770707, whole genome shotgun sequence.
ACCESSION   DAOSLL010000440 DAOSLL010000000
VERSION     DAOSLL010000440.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18816_length_4172_cov_3.770707"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(29..877)
                     /gene="argP"
                     /locus_tag="PLB25_16570"
     CDS             complement(29..877)
                     /gene="argP"
                     /locus_tag="PLB25_16570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator ArgP"
                     /protein_id="HOE43222.1"
                     /translation="MFDRAQLEAFSAVVQHGSFERAAKVLHVTRGAISQRIKALEDTL
                     STILLVREKPVVTTRKGDILLRHIRALKILEDGTISAILPTPHTRAPVLVAIAVNADS
                     LSTWFAPLVAQLLRRFKIALEIVSDDQDHTFFRLTTGEVLGCVSSAPDAMPGFEATPL
                     GAMHYLCVASPEFRRKYFKGGLSIAAALAAPAILFDRKDKLHDQYLAQLFGVKDTQYT
                     RHYLPSPTALLDGICAGLGYAMAPGLQIAPLLQTGQLVQLAPSCKVSVPLLLPCVEQT
                     KKGWQP"
     gene            1207..1467
                     /locus_tag="PLB25_16575"
     CDS             1207..1467
                     /locus_tag="PLB25_16575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HOE43223.1"
                     /translation="MAQNATYQLRIDQQTKDASFAVFRELGMTPAEGMRIFLTQVAKT
                     RAIPFPVNIPNEQTAKVLADTDAGIGLHRAKSLDDLFKQLDI"
     gene            1467..1733
                     /locus_tag="PLB25_16580"
                     /pseudo
     CDS             1467..1733
                     /locus_tag="PLB25_16580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
     gene            complement(1806..2201)
                     /locus_tag="PLB25_16585"
     CDS             complement(1806..2201)
                     /locus_tag="PLB25_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43224.1"
                     /translation="MPKALLCLLSALQLHVIGTQLPHEVWVALPQGGQTPALDYPKPR
                     IARLRGTACSEGIETVTEYGAPIRVYSIARTLTDCFKFRNKIGIDVALEALKDAWRTR
                     KISMAEVAHFAKINRVEKVTRPDLEAMLA"
     gene            2646..2813
                     /locus_tag="PLB25_16590"
     CDS             2646..2813
                     /locus_tag="PLB25_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43225.1"
                     /translation="MNVWMAPVRAGLAHSCRIERSDQATATLVPIQPGTGAASVVTAE
                     RSGEFGASKPK"
     gene            3454..>4172
                     /locus_tag="PLB25_16595"
     CDS             3454..>4172
                     /locus_tag="PLB25_16595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOE43226.1"
                     /translation="MVQISLPDGSQRDYAGPVTVAAVAASIGAGLAKAALAGKVAGKV
                     VDTSHLIEADAALSIITAKDADGLELIRHSTAHLLAYAVKELFPQAQVTIGPVIENGF
                     FYDFSFERPFTMEDLTVIEKRMTELAGKDEPVTRRVLPRDEAVAYFKGLGEHYKAEII
                     ASIPANEDVSLYREGTFEDLCRGPHVPSTGKLKHFKLMKVAGAYWRGDHRNEMLQRIY
                     GTAWASKEDLQQHLKMLEEAE"
BASE COUNT          903 a         1090 c         1241 g          938 t
ORIGIN      
        1 cccgatgagg gtagaaggct ggcagctctc agggttgcca gccttttttt gtttgctcga
       61 cgcatggaag taacaaaggc acgctcacct tgcaactggg cgcgagttga accagttgac
      121 cggtctgcag cagcggtgca atttgcaggc cgggtgccat ggcgtagcca agtccggcgc
      181 aaatgccgtc cagcagggcc gtgggtgatg gcaaataatg gcgcgtgtac tgggtgtcct
      241 tgacaccgaa gagctgcgcc aggtactgat catgcagctt gtctttgcgg tcaaacagaa
      301 tagccggcgc ggcaagagcg gcggcgatgg acagtccgcc cttgaaatac ttccgccgga
      361 actcagggga tgccacacac aggtagtgca tggcccccag cggcgtggcc tcaaagccgg
      421 gcatggcatc aggtgcgctg gaaacacagc ccagcacctc gcccgtggtc agccggaaaa
      481 aggtgtggtc ctggtcgtcg ctgacgattt ccagcgcaat tttgaaacga cgcagcaatt
      541 gcgcaaccaa gggtgcaaac caggttgaca gcgagtcggc attgactgca atggcaacga
      601 gaaccggtgc tcgggtgtgc ggcgtgggca ggatggcgct gatggtgccg tcttccagga
      661 ttttgagcgc cctgatgtgg cgcagcaaga tgtcgccttt gcgggtggtg acgacgggtt
      721 tttctctgac caataagatg gttgacagcg tgtcttcaag cgccttgatg cgttgagaaa
      781 tggcccctcg cgtcacatgc agcaccttag cggcacgctc aaagctgccg tgctgaacca
      841 cggcagaaaa agcctccagt tgcgcgcgat caaacatcgg gttcctctca tttttttgtc
      901 tttgggcaag actttgttta ttggttcaag tctttcgcga ggagtatcgc actctggttt
      961 tgcgctggct gtgctgcggc agaatgctac cgtttcagta gcttcttgcg caggtaggat
     1021 aagcgctaca gcctattttt atcactatga cttcctcagg atcggggctg ggtgcaggtg
     1081 gtgaagacgg aagatttgac ttgtgcgcag atgggggaag aggagttttg acaaggctag
     1141 agtttgactt tgcatgattt gcacatacca tgtcattaca tttacagaaa ctttgtgagt
     1201 atcgtcatgg cacaaaacgc gacctatcaa ctgaggattg accagcaaac gaaagatgct
     1261 tcttttgcgg tgtttcgtga attgggcatg acgcccgccg agggtatgcg gatttttttg
     1321 acccaggtag ccaaaactcg tgcgatccca ttcccggtca atattccgaa cgagcagaca
     1381 gccaaggtat tggccgacac ggatgcaggt attggattgc accgtgccaa gagcctggac
     1441 gatctcttta agcaacttga catctaatgc tcaagcctgt ttataccaac gcattcaaga
     1501 aggacatcaa gctggcgaaa aagcgttgtc tcgatatggc tgctttgcga tttgtgatga
     1561 ccgagattgc atgcgaaaaa tcactggcag caaagtacag aaatcacaaa ctcacaggaa
     1621 attggtaaga ccattgggag tgccacatct caccagattg gttgctggtg tacaaattgc
     1681 aacgtgatga agtcatcttt gagcgcacag gaagtcattc ggatttgttc tagcggttgt
     1741 caacgtcgca tgtgtcttgg ccagggtcag cagccgggcc aggatggacg caggcagatg
     1801 gctactcatg caagcattgc ctcaaggtca ggtcgcgtga ctttttcaac ccggttgatc
     1861 ttggcaaagt gggcgacctc tgccatgctg atcttgcgcg tgcgccatgc atccttgagg
     1921 gcttccagcg ccacgtcaat gccaattttg ttgcgaaact tgaagcagtc ggtcagcgtc
     1981 ctggcgatgc tgtagacccg gatgggtgcg ccgtactcgg tcacggtttc aatcccctcg
     2041 ctgcaggcag tgccacgcag acgggcgatg cgaggtttgg ggtagtcgag cgccggggtc
     2101 tggccgcctt gcggcagcgc aacccagact tcatggggca actgcgtgcc aatgacgtgg
     2161 agttgcagtg cggacagcag gcatagcaac gcctttggca cacgttggca aacctccagg
     2221 gcagtttggt gttcggtgat ttcggcatca ggctggccaa aattggcccg acggcagcat
     2281 catttggagt gggcctcacc gggcgaatcc gcagcgcttc aaagttgccg tctcgacctc
     2341 tcaccaactg acttgtctgt tggcctcgtc gtttcggtgc cggttgcgtt gccagagccc
     2401 gatgcggctc aatttgcgcc cggctatcca tctgcgctcc accatagtca tcccacagtg
     2461 ttggtccgcg tgctgcggat atgcgcgggg gctcggactc caccccgatg ggatcaagaa
     2521 tcttcctgat tggcacgcca tcggtaatga aggcagtcag gcgcatttgt gcacctgact
     2581 tcgggcacag cggcgggaaa acctcccaat ttcgggcgat cagactgccc acaggtggtg
     2641 cgccagtgaa cgtttggatg gcaccggtgc gggctggcct ggcacactcg tgccgcatcg
     2701 aacgctctga ccaggcaacg gcaacgcttg taccgattca gccaggcacc ggcgcagcca
     2761 gcgtcgtcac cgcagagcgg agcggcgagt ttggggccag caaacctaag tgacgatggt
     2821 ggtgcgcgtg cggtcgtggc tacacgccca gcctggagtc cggatcacac tggtggttgc
     2881 caatacaaaa ctgaatttca agcacgcttg agccttgatt tcattgcggc ctggggtggg
     2941 ttcgggtgtt tggcagtcga atccggctgc cccgtgttga tccacgctgc ggcacgccgt
     3001 gctgattcag gttgtcgact tgactggttc cggtcggatt gaggtcacag tcaggaccga
     3061 acgccaagtt gagtattcgc attcggcaaa gtggacgttc ccggaaaggg acgagaagct
     3121 gcgcatgttc cacttttgtg ttcataagca aaatgtgcct ccagcccttt tcccataagc
     3181 ggagatagct atctttacca tagcattcca tgggtttttc ggtggtgggt gcttgcggct
     3241 aaaatcttgg ggcacgattg aacaccgcac aagagcaaag tttgcatcgt tgcttgtgac
     3301 agcgccgctt gcgacagcca cttgattgag aaaaatagtg agtaaataag gctctagccc
     3361 gcgtggctta tgcgtaagat gcttctaata tcatagtaaa cgcggacttt tgccgcgttt
     3421 ttttgcctaa tttcaccgaa tccccagatc accatggttc aaatttcttt acctgacggc
     3481 tcgcagcgtg actatgcagg tccggtcaca gtggcagcag tggcggcttc gattggtgcc
     3541 gggcttgcca aggcggcgct ggcaggcaaa gtcgcgggaa aggtggttga taccagccac
     3601 ctgattgaag ccgacgctgc gttatcgatc attacagcca aagacgctga cgggctggaa
     3661 ctgattcggc attcgaccgc ccacttgctg gcctatgcag tcaaggagtt gttcccgcag
     3721 gcacaggtga ccatcggtcc ggtgattgaa aacggtttct tttacgactt ttcgtttgag
     3781 cgtcctttca ccatggaaga cctgacggtg attgaaaagc gcatgaccga gttggccggc
     3841 aaggacgagc cggtcacgcg ccgggtgctg ccacgcgatg aggcggtggc ctacttcaaa
     3901 ggactcggcg agcattacaa agccgagatc atcgccagca tcccggccaa tgaagatgtg
     3961 agcctgtacc gcgagggcac tttcgaagac ctgtgccgtg gccctcatgt ccccagcact
     4021 ggcaagctca agcacttcaa actgatgaag gtcgcgggtg cctattggcg cggcgatcac
     4081 aggaacgaga tgctgcaacg catttacggc accgcctggg ccagcaagga agacctgcaa
     4141 cagcatctga aaatgctcga agaagccgaa aa
//
LOCUS       DAOSLL010000441         4172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18817_length_4172_cov_3.676706, whole genome shotgun sequence.
ACCESSION   DAOSLL010000441 DAOSLL010000000
VERSION     DAOSLL010000441.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18817_length_4172_cov_3.676706"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            180..1064
                     /locus_tag="PLB25_16600"
     CDS             180..1064
                     /locus_tag="PLB25_16600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HOE43227.1"
                     /translation="MRKVPTQHGFTMVELIMVIVMMGVIGGIVAVFMKSPIDAYFDSA
                     RRASLTDVADTVVRRMARDIRKALPNSIRSAGSQCVEFIPTRTGGRYRADVGGGGDAL
                     DFSSAAGDTSFNMLGRNADLPASQQIVANDLIAIYNLGIAGADAYASDNTAAVTDASA
                     EAAGPPIETIISIAARKFPFESPGKRFHVITGTEQVVRYLCLDASGVDAQGRGLGRLV
                     RQVLSLPLAMGSTCPTTSGSTGVLSSALLANQVSSCNFNYSGSDLQRNALVSMSIGLT
                     DSGETVSLQHETHVSNAP"
     gene            1061..1516
                     /locus_tag="PLB25_16605"
     CDS             1061..1516
                     /locus_tag="PLB25_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43228.1"
                     /translation="MSPAKAELTRSLDGQHRQRGFAAVAAIFLVVVLAALGGFMLTFS
                     NTQQLTSAQDLQGTRAYWAAQAGLEWGIANSANCATFPPTSVATLPGASTTFDGGFTV
                     SVDCTLKSFTEAAASANVNIYSLNAVARSGGSVGTIGYVERSLSVVIEK"
     gene            complement(1987..2835)
                     /locus_tag="PLB25_16610"
     CDS             complement(1987..2835)
                     /locus_tag="PLB25_16610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HOE43229.1"
                     /translation="MTNHDACYLCSRRRLLLQGPALWLAFAGAAPQQSQAREGVDVGR
                     NSMFAKLVPAEIIEKSAAQQYLQMMKQAASQNALAGKEHPQLRRLRAIAQKTIVHTLG
                     WNPRAANWKWEVNLIGSKQINAFCMPGGKIAFYSGILDGLKLTDDEVATVMGHEVAHA
                     LREHARERIGKNAATGVGATLLSQALGLGQLGQTVTNYGAQLLTLQFSRSDESEADLV
                     GMELAARAGFDPRAGVSLWQKMAAASRGAPPQWLSTHPSGSSRITDIEANLPKVLPLF
                     ARSRPQ"
     gene            2989..>4172
                     /locus_tag="PLB25_16615"
     CDS             2989..>4172
                     /locus_tag="PLB25_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014427662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase"
                     /protein_id="HOE43230.1"
                     /translation="MKNPLIIASIDACVAHILNTVPGTIVMGIPLGIGKPNPLVNALY
                     SRIKALPSRKLRIITALSLERPVGHSDLEKHFLQPLVERVFGDYPDMDYVKDLRAGQL
                     PANIEVQEFFMKTGDYLGNADAQRGYISTNYTFAARDMVVQGLNVIAQAVGAKGIGDS
                     LRLSLSSNPDITEEVIECVRATGQPVLAVGVINQKMPFMPNGAEVSPGFFDVVVTDPT
                     ATHDLFAPPNNRVSPADYAIGLHASSLVVDDGTLQIGIGSLGDAVGQALIVRDRHSAD
                     YRHILESICPTGLDGRELGRFNIGLYGCSEMLVNAFLRLIEAGIIRREVYDDAVLQRL
                     LNSGRIKDDCVTPATLQALLDEGRIRSPLAEEDVTFLKRFGILRPEVKISGQQLRFED
                     QH"
BASE COUNT          847 a         1219 c         1244 g          862 t
ORIGIN      
        1 gagtgccgcg ctgaatgccc tggcggtgaa gaaagtcacc gtgtctgtgt ccagatccgg
       61 cgacaccctc agcctgactg gttaccgggc caactattag gcaacggctt gatgcggcgc
      121 tgccctttca aatccaatcc ggccagatcg tttgtgctgc tcctgcagcc cggcacgcca
      181 tgagaaaagt cccgacccag cacggcttta ccatggtcga gctgatcatg gtgatcgtca
      241 tgatgggggt gattggcggc atcgtggcag tgttcatgaa aagcccgatc gacgcctatt
      301 ttgacagtgc ccgccgtgcc agcctgaccg acgtggccga taccgtggtg cgccgcatgg
      361 cgcgagacat ccgcaaggcg ctgcccaaca gcatccgcag cgctggcagc cagtgcgttg
      421 agttcattcc cacccgcacc ggcgggcgct accgcgccga tgtcggcggc ggtggtgacg
      481 cgcttgactt cagcagcgcc gcaggggaca ccagttttaa catgctcggg cgcaacgccg
      541 acttgcctgc atcccagcaa atcgtcgcca atgacctgat tgccatctac aacctgggca
      601 ttgccggtgc ggatgcctat gccagcgaca acaccgcagc cgtcaccgat gccagtgccg
      661 aggcggccgg gccaccgatt gagaccatca tcagcattgc tgccaggaag tttccatttg
      721 agtcgccggg caagcgcttt cacgtgatca ccggcaccga gcaggtggtg cgctacctgt
      781 gccttgatgc cagtggagtt gatgcccagg gtcgtggcct tggccgcctg gtgcggcaag
      841 tcctgtcttt gccgctggca atgggcagca cctgcccgac cacgtctggc agcaccggcg
      901 tgctgagctc ggcgttgctg gccaaccagg tgagttcttg caacttcaac tacagtggct
      961 cggatctgca gcgcaacgcc ttggtcagca tgtccatcgg gttgaccgac agcggtgaaa
     1021 ccgtcagcct gcagcacgaa acgcatgtga gcaacgcacc atgagtcccg ccaaggctga
     1081 actgacccgg tcgctcgatg gccagcaccg ccagcgcggc tttgctgctg ttgccgccat
     1141 ttttttggtg gtggtgctgg cagcactggg tggcttcatg ctgacttttt caaataccca
     1201 gcagttgact tcagcacagg acttgcaggg cacgcgggct tactgggcgg cgcaggccgg
     1261 gctggaatgg ggcatcgcca attcggccaa ctgcgcgaca tttccgccta cctcggtggc
     1321 aactttgcca ggtgccagca ccacctttga tggcggtttt accgtcagtg tcgattgcac
     1381 cctgaagtcg tttacagagg ccgctgcttc agccaacgtc aatatttaca gtttgaatgc
     1441 ggtggcccga tcaggtggca gcgttggcac cattggctat gttgaacgca gcctgtcggt
     1501 ggtcatcgaa aaataaggcc ttcatgatca aatagacctg tagcccccgt cagtcatgcg
     1561 ttggcagctc ctaaattggt agctatatag aagcatcaaa cctgaactaa gggcgcttgc
     1621 gaagacggga atctggcccg ccaccgatca acaggcaggc atctgaatca tggtcgcgca
     1681 accagcaacg cgggaatccg caaacgcaac tggagccggt ccgtcgtatg gtgcatcggc
     1741 agcgaaccca ctgcaacagc ccaatttgga acggcttttt gaatcacatc aggctatggg
     1801 ccggcaaatc aggcacaagc tggtcgcgct gggattgctt ggtgcgacag gctcctggca
     1861 caaggggcag cagatggcgt gcgctacctc cctgtctggt gtgatcgacg caattggcac
     1921 atccagcacg caccggggca tggccacatt gatcgacgat ggcaccgctt tcaggtgccc
     1981 gatgcgctat tgcggccggg accgcgcaaa cagcggcaga acctttggca ggttcgcctc
     2041 aatatcagtg atgcggctgc tgccggacgg atgggtagac agccactgtg gtggcgcacc
     2101 cctgctggcg gcagccattt tttgccacag actgacaccc gcacgggggt caaagccagc
     2161 gcgggctgcc aactccatgc cgaccaaatc ggcctcggat tcatcgctgc ggctaaattg
     2221 caacgtcagc aactgggccc cgtagttggt gacggtctgg cccagttgcc ccaggccgag
     2281 cgcctggcta agcagcgttg cgccaacgcc ggtggcggca ttcttgccga tgcgctcgcg
     2341 cgcgtgttca cgcaatgcat gggcgacctc atggcccatg acggtggcga cctcatcgtc
     2401 ggtgagtttg agcccgtcga gaatgccgct gtaaaacgca atcttgccgc ctggcatgca
     2461 aaacgcgttg atctgtttgc tgccaatcag gttgacctcc catttccagt tggccgcacg
     2521 cgggttccag cccagcgtgt gcacaatggt tttctgtgcg atggcgcgca ggcgccgaag
     2581 ctgtggatgt tccttgccag ccagggcgtt ttggcttgcg gcctgtttca tcatctggag
     2641 gtattgctgc gccgcagact tttcgatgat ctcggctggc accagtttgg caaacatgga
     2701 gttgcgcccg acatcaaccc cttcgcgggc ctggctttgc tgcggcgcgg cccctgcaaa
     2761 cgcgagccac agggccggtc cctgaagcaa caagcgtcgc cgggagcaca ggtaacacgc
     2821 gtcatgattt gtcatcggca gacagtggtg ggttggctga atcactgaga tggtaagaaa
     2881 ttggtggaac aagtccaaag cgcaaaaagc gactagcgca attgtgagcc aaagcgcaaa
     2941 cttcaagtcc tgaagcattt gcacaagctg caagcaaagg aataagacat gaaaaatccg
     3001 ctcatcatcg cgtccattga tgcctgtgtg gcgcatattc tgaacactgt tccaggtacc
     3061 attgtgatgg gaatcccgct tggcattggc aagccaaacc cgctggtcaa tgcgctttac
     3121 agccgcatca aggcgcttcc gtctcgcaaa ctgcgcatca tcactgccct gtcgctggag
     3181 cgaccggtcg gacacagtga ccttgaaaaa cactttttac aacccctggt agagcgggtg
     3241 ttcggcgact acccggacat ggactatgtg aaagacctcc gggccgggca gttgccagcc
     3301 aatatcgaag ttcaagagtt tttcatgaag acgggtgact acctgggcaa tgcggacgcg
     3361 caaagggggt acatctccac caattacacc ttcgcggcgc gtgacatggt ggtgcagggg
     3421 ctcaatgtga ttgcgcaggc tgtgggagcc aaaggcatag gcgatagttt gcgtctgagc
     3481 ctgtcgagca acccagacat taccgaagag gtgattgaat gcgtgcgagc aaccgggcaa
     3541 ccggttctgg ccgtcggggt catcaaccag aaaatgccct ttatgcccaa cggtgcagaa
     3601 gtcagccccg ggttttttga tgtggtggtg acagacccga cagcgaccca tgaccttttt
     3661 gcgcccccca acaaccgggt gagtccggca gactatgcaa ttggcctgca tgcctcaagc
     3721 ttggtggttg atgacggcac gttgcagatc ggcattgggt cactgggtga tgccgtcgga
     3781 caggcactga ttgtgcggga ccgccacagc gctgattacc ggcacattct tgagtcaatc
     3841 tgcccgaccg gtctggatgg acgcgagctg ggtcgcttca acatcggctt gtatggctgc
     3901 tctgagatgc tcgtcaacgc tttcttgcgc ctgattgagg ctggcatcat ccgccgcgag
     3961 gtgtatgacg atgcggtgct gcaacgtctg ctcaacagcg gacggatcaa ggatgactgc
     4021 gtcacccccg cgactttgca ggctttgctc gatgagggtc gcatccgttc gccactggcc
     4081 gaggaagatg tcacctttct caagcgcttt ggcatcttgc gacccgaggt aaaaatcagc
     4141 ggtcaacaac ttcggtttga agaccagcat tg
//
LOCUS       DAOSLL010000442         4166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18848_length_4166_cov_4.604719, whole genome shotgun sequence.
ACCESSION   DAOSLL010000442 DAOSLL010000000
VERSION     DAOSLL010000442.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18848_length_4166_cov_4.604719"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            868..1152
                     /locus_tag="PLB25_16620"
     CDS             868..1152
                     /locus_tag="PLB25_16620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43231.1"
                     /translation="MASDLKLLRRRAEDSHQMRAALPLDLGKEMMPEDAQLLFHELRV
                     HQIELEMQNEELRRTKLALEVARERYFDLYDLAPVGYCTPTSSARAICSR"
     gene            1312..1893
                     /locus_tag="PLB25_16625"
     CDS             1312..1893
                     /locus_tag="PLB25_16625"
                     /inference="COORDINATES: protein motif:HMM:NF013502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOE43232.1"
                     /translation="MNNFPIICVGGSVGGLDAYIRLLKNLPPDMDVAIVIVNHITIMA
                     TQLHEVLPRFSSMPVELIADNLLVQPDQVFIFPANRDLECRGRFFWLRPISKPQGWPD
                     VITVFLRSLTANWGGQLIAVIVSGYDGDGAAALCGIREAGGITIAQKPSTATQPDMSD
                     SAIDSGCIDYVLSPEDIAQTIVRIAGGNQTAAL"
     gene            2067..2909
                     /locus_tag="PLB25_16630"
     CDS             2067..2909
                     /locus_tag="PLB25_16630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010393339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE43233.1"
                     /translation="MNLCVDVLAASILIVDDKSANVQLLEQLLLEAGYTQVSSTMKPE
                     AVCAMHRTHRYDLILLDLQMPVMDGFAVMASLKTNVEDDYLPVIVLTAQPGHKMRALL
                     AGAKDFISKPFDLVVVKTRIHKMLEVRLLYKKLASYNELLEATVAERTAELRESQARY
                     RSLAELACDWYWEQDENGAFTKVSGPVLNMLGLRVSPLHADEEQLLPSGWNDEERIKL
                     HEIIGNRQPFLDFVLHRANPEGYQQVFQVSGEPMFDRLCRFVGYRGIGLEISVKSKLP
                     IPWV"
     gene            complement(3937..>4166)
                     /locus_tag="PLB25_16635"
     CDS             complement(3937..>4166)
                     /locus_tag="PLB25_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HOE43234.1"
                     /translation="TAIPLLLFAAGARRIPMATLGILQYISPSLQMLLGVWLYGEAFD
                     PARAVGFYLIWLALVVYSIDGLRNSRRQLSK"
BASE COUNT          924 a         1165 c         1171 g          906 t
ORIGIN      
        1 cagtgcgctg ctgcaactgg gctgcgaact ggcacaaggc tacggcattg cccgaccgat
       61 gcccgctgag cagttgccac tgtgggtgac atcttggcag cccgatgttg cctggagtac
      121 ggcagacaaa tgaacataaa gtacctccag actccgtttg gattgcgctg acagctaacc
      181 gcgcagacaa caaccatgca ctgttgattt ccatggtttt attgaccgat cactgcgtgc
      241 tgtttttgaa gatatgcctg ttgtgatgat gatatggaaa atgttttccg ggtttgccat
      301 cgtggccgta cccgccacgc cagtgcttgc ggtggcaaag cctacaaatc actcgttgca
      361 tgcatgaagc cagaaacaat gttttatgcg gaatggctga gtttttcgcg atactttatg
      421 cgtctattgc ttaaagggct tttccggtcc atcgctcccc agacctctta aacccccttt
      481 tgccgagttc aagtgtgcga tgctgcgtga gcgcacgcgc aatggccttg atgccgcccg
      541 aaaagaaggg cgtattggtg tgcgacggcc aaaacaaaaa atgaaccaag agcgggggat
      601 tgtgctgtta gtcaattcct ggcaaaagac agttgcatcg agctgccagc atcgacactt
      661 gctagtggtg ccggattggc aggcgcagtg cccatttttg ggtcaacatg ccgctgccag
      721 gtgggccgct gcgggcagtt tacggccgct gcgccagcag ttcggcaggc tccagcgcca
      781 gccatcacgc ggtggcagcg cacaatgaac caaccgggga ataaaaacat gtagattcct
      841 gccagatatc tacccgacgc gacgcacatg gccagtgact taaagctgct gcgccgacgc
      901 gccgaagaca gtcaccaaat gcgcgcggcg ttgccgcttg atctgggcaa agagatgatg
      961 cccgaggacg cacaactact gtttcatgaa ctgcgcgtgc accagattga gctggagatg
     1021 caaaacgaag agctgcgtcg cacgaaattg gcacttgagg tggcgcggga acgctacttt
     1081 gacctgtatg acctggcccc ggtgggctac tgtacgccaa cctcgtccgc cagggccatc
     1141 tgctctcggt agcgggcttc aacgtcgcac ctgcagggcg cagccattcc acaaggttgg
     1201 ccagcttcaa gtcgtccaaa cgtgagcagg caagtttgga gttgaggcct tcgccctgcc
     1261 cagccgcgct gcgggtgacc gattcgggcg cgttcaactg aagaatccag gatgaacaat
     1321 tttccgatca tctgcgtggg cggctctgtc ggcgggctgg atgcctacat tcgcttgctg
     1381 aaaaatctgc cacccgacat ggatgtggcg attgtcatcg tcaaccacat caccataatg
     1441 gcgacccagt tgcacgaggt gctgccgcgc ttttccagca tgccggtcga gctgattgcc
     1501 gacaacctgc tggtgcagcc cgaccaagtg ttcatttttc cggccaatcg cgatcttgaa
     1561 tgtcgagggc ggtttttttg gctcaggccg atctccaagc cgcaaggctg gccggatgtg
     1621 atcaccgtgt ttttgcgctc gctcacggcc aactggggtg gccagttgat tgccgtgatt
     1681 gtgtcgggct acgacggcga tggcgcggct gcgctgtgcg gcatccgcga ggccgggggc
     1741 ataaccattg cgcaaaaacc cagcaccgca acccagcccg acatgtccga cagcgccatc
     1801 gactccggtt gcattgacta tgtgctgtcg cctgaggaca ttgcgcagac catcgtgcgc
     1861 atcgcgggcg gcaaccagac agccgcctta tgagttccag cacacagacg gcgctagcgc
     1921 aatcactcag catcagcgca tgctgaggat cacggcgagc acttgagaaa ttgatgtaga
     1981 aatgtctttt ggcccatacg catactgagc tgacagatcc tgaattggta gtaaaaaggt
     2041 cttcaagaag gcacaaggaa cacccgatga acctctgcgt tgatgttttg gccgccagca
     2101 ttttgattgt ggatgacaag tcggccaatg tgcagttgct tgagcagttg ctgctggagg
     2161 ctggctacac ccaggtgagc tccaccatga aacctgaggc ggtgtgcgcc atgcaccgca
     2221 cgcaccgtta cgacctgatt ttgctggacc tgcaaatgcc ggtgatggac gggtttgccg
     2281 tgatggcgag cctgaaaacc aatgtcgagg acgattacct gccagtgatt gtgctgaccg
     2341 cccagcccgg ccacaaaatg cgtgccctgc tggccggggc caaagacttc atcagcaagc
     2401 cgtttgacct ggtggtggtg aaaacccgca tccacaagat gctcgaagta cgactgctgt
     2461 acaaaaaact ggccagctac aacgaactgc tggaagccac cgtggccgag cgtaccgccg
     2521 aactgcgcga aagccaggcg cgctaccgca gcctggcaga actggcttgc gactggtact
     2581 gggagcagga cgaaaacggc gcttttacca aagtgtccgg gccggtgctc aatatgctgg
     2641 gcctgcgagt cagccccttg cacgccgatg aagagcagtt gctgcccagc ggctggaacg
     2701 atgaagagcg catcaagctg cacgagatta ttggcaaccg gcagccgttt ctggactttg
     2761 tgctgcaccg ggccaacccg gagggctatc agcaggtttt tcaggtcagc ggcgagccga
     2821 tgtttgaccg gctctgtcgc tttgtcggtt atcggggcat cgggcttgaa atcagcgtca
     2881 aatcaaaact cccgattcct tgggtttgaa tcacaaaccg ccgaaatttc cgtcacacct
     2941 tgtccagcag cttttgtccc gcctcccttg gtgttccatt gccattgaca tacatataaa
     3001 gcgttgtggt cgtgatacct agccgtttgg cgacattgcc tgcgtgtgct tatggattgg
     3061 ccaacgcagt catggccatt gccaaagttg agtgatccat ttttcttggt cgcacgcgtt
     3121 gacgaccacg cggtcttgcc gaagcaagcc cggcatgcgt gcacttggcg aagaattccc
     3181 gctcgaattc cgccagtgcc gcaaaaatcc caaaaaagta accgcccttt ggcggtggtc
     3241 gtgtcgatct gagcgccgac accggcttcg gccaatactt tcaatccaat gttacgggta
     3301 agcagttcat ctaccgtcgt atatagaaaa gccgagtcgg aagtggtgcc tgcgccgcac
     3361 acacagacca gcaaccaaga ccacctaaat ttctgacttc gacgctgctg ctgtggctac
     3421 cgctcgactc tggagttagc caaagcaggt acaacttatg aagaatgcgt acaagttgtg
     3481 aggtttccct cgaaaactgg ctaaccagca tggctgaacc cagtatccgg tggacctatc
     3541 ggccgatggc accgacgctc aacggcgttg gtagactgca agcctgtacc ttgctggcaa
     3601 gccactttcc accccacacg caagactggg aaaatatggt gcaagtcaaa gagcgcattt
     3661 actgcacttt tgctttgctc acttgtttgc ggtagcggtc gcagccagcc agatgacgga
     3721 aaaatttgcc acctgccgat agtttttgcc aatgccaaga ttcccctgct aactcttggc
     3781 cctgctgatc cggctttgtc ttactacgga tttgccgtct tgcattccga cgagagcaaa
     3841 atactggttt tcgttgctgt gaagctcgac agcgatttga gggctaagac accccaagag
     3901 cccttgatgc gttcagccat gatgcaagcg ttgtgtttat ttggatagct gcctgcggct
     3961 gttgcgcaag ccgtctatgc tgtaaaccac caaggccaac caaatcaggt agaaccccac
     4021 ggcacgggct gggtcaaacg cctcgccgta cagccatacg cccagtaaca tttgcaggct
     4081 gggcgaaatg tattgcagga tgcccaacgt ggccatgggg atgcggcgtg caccagcggc
     4141 aaacagcagc aagggaatgg ccgtga
//
LOCUS       DAOSLL010000443         4156 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18912_length_4156_cov_4.742258, whole genome shotgun sequence.
ACCESSION   DAOSLL010000443 DAOSLL010000000
VERSION     DAOSLL010000443.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4156)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4156
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18912_length_4156_cov_4.742258"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="PLB25_16640"
     CDS             <1..250
                     /locus_tag="PLB25_16640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43235.1"
                     /translation="GRSCASSSRLPRRAASGPLGRVQLRNQGSPVHGLQPLMAQPQTI
                     ARNTCRTVKSDDAPTFELITTPHQQHKRALELIGQIKP"
     gene            complement(322..1557)
                     /locus_tag="PLB25_16645"
     CDS             complement(322..1557)
                     /locus_tag="PLB25_16645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013699581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGE family epimerase/isomerase"
                     /protein_id="HOE43236.1"
                     /translation="MSTRHFTPPAPDFGNPEFLLRHMQSLMDFYLPQAHDPSGGHYQY
                     FLDDGTIWDHDTRHLVSATRYAVTHSMLWRATGEPRYKDGLAHALRFLADAFCIGPGE
                     GYYWMVEWRDGQRQRVIDDTRQMYGLAFVMLAGAHGLLCGLEQGRILLEDAFATAEQH
                     FWLPEHGLYADDASADWVVSSYRGQNPNMHACEAMLAAFEGTQESRYLARARTLADSV
                     VRKLGDMQGGRICEHYDTHWVPDWLYNLNDSSNIFRPWGYQPGHFTEWAKLLLQLDHF
                     DPQPWHLEKARFLFDEATRTAWDHDNGGFYYGVGPDEQICDANKYHWVQAETLSAAAL
                     LAKATGEVSYLQWYERTWGYCWAHFVDHEQGAWFRILDRRNLNHTREKSPAGKVDYHP
                     IGACFDVLRAHQQIGYKTR"
     gene            complement(2097..3101)
                     /gene="hrcA"
                     /locus_tag="PLB25_16650"
     CDS             complement(2097..3101)
                     /gene="hrcA"
                     /locus_tag="PLB25_16650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HOE43237.1"
                     /translation="MLDDRAKLLLKALVERYIADGQPVGSRTLSKASGIELSPATIRN
                     VMSDLEELGLIASPHTSAGRIPTARGYRLFVDTMLTVQRGLLGEHYLAADQPQKVISN
                     AANLLSSLSQFVGVVVAPRRSSVFRHIEFLRLSDRRLLVIIVSPEGDVQNRVIFTEAD
                     YTQGQLLEAANYLNANYVGLAIEQVRERLKGEVDSLRAEIACLMHAAVAANSDVLTEA
                     QDEVIISGERNLLSVSDFSHDMSHLRRAFDLFEQKAQLLRLLDGTGQAEGVRIFIGGE
                     SQVVPFEDLSIVSAPYEVDGLVVGTLGVIGPTRMAYDRMIQIVDITSKLVSNALSHHK
                     "
     gene            3149..3391
                     /locus_tag="PLB25_16655"
     CDS             3149..3391
                     /locus_tag="PLB25_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43238.1"
                     /translation="MKVLGTGEKMVELNVVTFWGLCLLAAMGLIRAAIDVFTKTDNVE
                     HTHWTKPAKPDAPEGMEWVLIKKRDLRKPDIDDPLN"
     gene            3760..>4156
                     /locus_tag="PLB25_16660"
     CDS             3760..>4156
                     /locus_tag="PLB25_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HOE43239.1"
                     /translation="MTPKFQLVALIGKFHTHASASSIANSRNAIEAVAHFLHDQGCEV
                     VLEHDSALEMGLTGYQQLTVAQIGEQCDLGLVVGGDGTMLGIGRQLAAYGVPLIGINQ
                     GRLGFITDIPYDSFTASLGKMLQGEYVEDH"
BASE COUNT          978 a         1151 c         1165 g          862 t
ORIGIN      
        1 gggcaggagc tgtgcgtcgt cgtcgcgcct acccagacgc gccgccagcg gcccactcgg
       61 gcgcgtccaa ctgaggaatc aaggttcacc ggttcatggt ctccagccct tgatggcaca
      121 gcctcaaacc attgcgcgca atacctgtcg caccgtcaaa agcgacgacg cgcccacttt
      181 cgagctgatc acgacacctc accagcagca taaacgggcg ctcgaactga tcggtcagat
      241 caaaccgtag tcagaacgtg aaaccgcaat tgccggtggt ctcggtgggg aaacccgggc
      301 tttttggcaa agaacttcag cttagcgtgt tttgtagccg atttgctggt gcgcgcgcaa
      361 cacgtcgaaa caggcaccaa tcgggtgata gtcaaccttg ccagcagggc ttttttcgcg
      421 cgtgtggttg aggttgcgcc ggtccagaat ccggaaccag gcaccctgtt cgtgatcgac
      481 aaaatgcgcc cagcagtagc cccaggtgcg ctcataccat tgcaaatagc tgacctcgcc
      541 ggtggctttg gccagcaggg cggcggcgct cagggtttcc gcctgcaccc agtggtactt
      601 gttggcatcg caaatctgct cgtctgggcc gacaccgtag tagaaaccac cattgtcatg
      661 atcccaggcg gtgcgggtgg cttcgtcaaa caagaagcgc gctttttcga gatgccaggg
      721 ctgcgggtca aagtggtcaa gttgcaacag cagcttggcc cactcggtga agtggccggg
      781 ctggtaaccc cagggtcgga aaatattgga actgtcgttc aggttgtaaa gccagtcggg
      841 cacccagtgg gtgtcgtaat gctcgcagat gcgcccaccc tgcatgtcgc ctagcttgcg
      901 aaccaccgaa tctgccagcg tgcgggcgcg ggccagatag cgggactctt gggtgccctc
      961 aaacgctgcc agcatggcct cgcacgcatg catgttgggg ttctgacccc ggtaagagga
     1021 caccacccag tctgcgctgg catcgtccgc atacaagccg tgttcaggca accaaaaatg
     1081 ctgttccgcc gtcgcaaagg catcttcgag cagaatccgg ccctgctcca gaccgcacag
     1141 cagcccgtgc gcgcccgcca gcatcacaaa ggccaggccg tacatctgcc gggtatcgtc
     1201 gatcacccgc tgcctctgcc cgtcgcgcca ctcgaccatc cagtaatagc cttcgcctgg
     1261 accgatacaa aaggcatcgg ccaggaagcg tagcgcatgc gccagtccgt ctttgtaacg
     1321 cggctcgcca gtggcacgcc agagcatgct gtgcgtgacg gcatagcggg tggcgctgac
     1381 caagtggcgg gtgtcatggt cccagatggt tccgtcatcc aggaaatact ggtagtggcc
     1441 gccagatgga tcgtgcgcct gtggcagata aaaatccatc aggctctgca tgtggcgaag
     1501 caaaaattcc gggttgccaa aatcgggcgc tggcggggta aagtggcgag tactcaattt
     1561 agctctcctg gttaaatact tggcgcgacc gctggccgca gccaaagggt gtcgcttcaa
     1621 cggctggtgg attgaagttg acctggacca tatcctcagc ctcaacaagg aggcagaccg
     1681 ccacgcagcg gcgcatgcgg catggcaatt ttcctcgata agtttcatga tgtcatcgcc
     1741 gctgttgttg aaggatttga ctggctgatc tttaagggac acctgacctc acccttgccg
     1801 cgagctgcct ttgggcgcta tttgtccaag cgtggcgcac gacacaaaga tgcgggtaac
     1861 tccgtttcca aatcatccct gtcgttgata tctcgacttg ccttgatctt gccttgcgtt
     1921 ggcaatgtct gcgccagggt gcctggtcac gaatgactca gaaatggtat cagtgaccca
     1981 aacaagcggt caaccgagga agccgggatg aatgcgcgtt ggacaggact gcgcagtgac
     2041 aaattttcat cgtgtggtgt cacgcgtcgc ccgtttcaac cagtcaattt caaggcttac
     2101 ttatggtggc tcagggcgtt gctgaccaat ttggaagtaa tgtcaacgat ttgaatcatg
     2161 cggtcgtagg ccatgcgggt tggcccgatg acaccgagcg tgccaaccac caaaccatcg
     2221 acttcatacg gggcgctgac gatcgacagg tcttcgaacg gcaccacctg gctttcaccg
     2281 ccgatgaata tgcgaacccc ctcggcttga ccggtgccat caagcaggcg cagcaactgg
     2341 gccttttgct caaacagatc aaaagcacgc cgcaaatggc tcatgtcgtg cgaaaaatca
     2401 ctgacggaca gcagatttct ttcgccggaa ataatgactt cgtcctgtgc ttcggtcaag
     2461 acatcggaat tggcagcaac cgcagcgtgc atcaggcagg caatctcggc ccgcagtgaa
     2521 tccacctcgc ctttgagtcg ctcccgcacc tgctcgattg ccagaccgac gtagttggcg
     2581 ttcagatagt tggcagcttc cagcagttgc ccctgggtgt aatcggcctc ggtaaagata
     2641 acccggtttt gcacatcgcc ttctggcgac acaatgatga ccagcaggcg ccgatctgac
     2701 agacgcaaaa actcgatatg ccgaaaaacc gagcttcgcc gcggagccac caccacaccg
     2761 acaaactgcg acaggctgga aagcaaattg gccgcgttgg agatgacctt ttgcggctga
     2821 tcggctgcaa gatagtgttc gccgagcagc ccgcgctgca ccgtcagcat ggtgtctaca
     2881 aacaagcggt agccgcgtgc cgtggggatg cgcccggccg aggtgtgcgg gctggcgatc
     2941 agaccgagtt cctccaggtc agacatgaca ttgcggatgg tggcaggcga taactcaata
     3001 ccggatgcct tggacaaggt tcgtgacccc accggttgcc cgtcagcaat gtagcgttct
     3061 accagcgctt tgagtaataa cttggcacga tcatcgagca tgatttcatt ttaaatcttg
     3121 catggtgatg accccttaat ttggttaaat gaaagtctta ggaacgggag aaaagatggt
     3181 cgaattgaac gtggtcactt tttgggggct ttgcctactt gctgcaatgg gcttgattcg
     3241 cgcagcaatt gacgtgttta caaagacaga caacgtcgaa cacacacact ggacaaaacc
     3301 agcaaaacca gacgcacccg agggaatgga atgggttttg ataaagaagc gagacttgag
     3361 aaaaccagac attgatgacc ccttaaattg acaacttgtc ataaaagaga ttgttaacca
     3421 gtttgtgacg agtcgtcaac ataaattttg acgacaagtc ataaacttcc ctaattaaca
     3481 ggagaagtca tggccaacac aatgcaactc ttaacgaaag cgttagaact gaaacgagcg
     3541 tcaagatgga gcgaagaact tgacatagat gtgtcaacaa tcgcccaagc aaagaaacgc
     3601 ggacacctaa gcccagcgat agcaggcgca atagcggaca agctaggcga aaacgtccaa
     3661 caatggatag tcatcgccgc aatggaatca gaacgagaca gcccagtaaa aacgcaccta
     3721 ctgcgccgcc ttacatcact ttagtgatgt aatttgagaa tgactcccaa attccagttg
     3781 gttgccctga tcggcaagtt ccacacgcat gcctcggcat cgtcgattgc caattcgcgc
     3841 aacgcgatcg aagccgttgc acattttttg cacgatcaag gatgcgaggt ggtgcttgag
     3901 catgactctg ccctcgaaat ggggttgacc ggctaccagc aattgacagt ggcgcagatt
     3961 ggcgagcagt gcgatctggg cctggtggtg ggtggcgacg gcaccatgct ggggattggc
     4021 cgacagttgg ctgcctacgg tgtgccgctg attggcatca accaggggcg gttgggcttc
     4081 attaccgaca ttccctatga cagctttacc gccagtctgg gcaagatgct tcaaggcgaa
     4141 tatgtggaag accatc
//
LOCUS       DAOSLL010000444         4151 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_18945_length_4151_cov_3.594482, whole genome shotgun sequence.
ACCESSION   DAOSLL010000444 DAOSLL010000000
VERSION     DAOSLL010000444.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18945_length_4151_cov_3.594482"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="PLB25_16665"
     CDS             complement(<1..650)
                     /locus_tag="PLB25_16665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE43240.1"
                     /translation="MTTKIALPLATRRADLMSDLAKPTQYDIAVVGGGATGLGVALDA
                     VVRGLRVVLIDSHDFAKGTSSRSTKLVHGGVRYLAQGNISLVQEALQERMRILNNAPY
                     LAQPQTFVMPTYHWWEAPFYGIGLKMYDALAGRAGLGKTELLSRAETLKYLPTLQARG
                     LKGGVKYWDGQFNDARLALALARTAAARGALMINYCRAIELTYDNGKVSGLVCEDH"
     gene            complement(1076..2806)
                     /locus_tag="PLB25_16670"
     CDS             complement(1076..2806)
                     /locus_tag="PLB25_16670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019699976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE43241.1"
                     /translation="MKLRYTALSVAIACALASQGWADEAAAKKWIDSEFQPSTLSKDQ
                     QTAEMKWFIDAAKKLQAKGVKEISVVSETITTHEYESKTLAKAFTEITGIVVKHDLIQ
                     EGDVVEKLQTSMQSGKSIYDGWITDSDLIGTHYRYGKVLNLTDYMSGSGKEWTNPGLD
                     LKDFIGTSFTTAPDKKMYQLPDQQFANLYWFRADLFARQDLKAKFKVKYGYDLGVPLN
                     WSAYEDIAAFFSEDVKTIDGKPIYGHMDYGKKDPSLGWRFTDAWLSMAGTADIGIPNG
                     KPVDEWGIRASADGCNPQGASTARGGATNSPAAVYALTKYIDWMKKYSPKEAIGMTFG
                     EAGPVPAQGQIAQQIFWYTAFTADMIKPGLPVVNADGTPKWRMAPGPNGPYWKQGMQN
                     GYQDVGSWTFFKDHDANKVAAAWLYAQFVTAKSTSLKKTIVGLTPIRESDIQSKAMTD
                     MAPKLGGLVEFYRSPARVAWTPTGTNVPDYPKLAQLWWKNVAVAVTGEKTPQAAMDNL
                     ATEMDDVMARLERSGMAVCAPKLNAKGDPGKWLSDKGAPWKKLANEKPKGETIAYETL
                     LSAWKNGKVR"
     gene            complement(2894..3202)
                     /locus_tag="PLB25_16675"
     CDS             complement(2894..3202)
                     /locus_tag="PLB25_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2160 domain-containing protein"
                     /protein_id="HOE43242.1"
                     /translation="MFEWMAWTTPVAVFFVCIGLMLFGMTVWEINAPTSLRRGFLPLE
                     TTRGDRLFIGLLTAAYVNLAFVGISGKLVVWMELESEPSIWISFVLSMGLLALIMRKG
                     "
     gene            complement(3246..4058)
                     /locus_tag="PLB25_16680"
     CDS             complement(3246..4058)
                     /locus_tag="PLB25_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007831605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE43243.1"
                     /translation="MNSPGFQKRTIFLLAYIVFALLPVYWMVNMSFKTNDEILASFSL
                     FPQHFTWANYRTILTDVSWYSGYINSLIYVGINTVISITVALPAAYAFSRYSFLGDKH
                     VFFWLLTNRMTPPAVFLLPFFQLYTTVGLMDTHLAVALAHLLFNVPLAVWILEGFMSG
                     IPREIDETAYIDGYSFPKFFIRIFIPLIKAGVGVAAFFCFMFSWVELLLARTLTSVNA
                     KPIVATMTRTVSASGMDWATLAAAGVLTIVPGAIVIWFVRNYIAKGFAMGRV"
     gene            complement(4051..>4151)
                     /locus_tag="PLB25_16685"
     CDS             complement(4051..>4151)
                     /locus_tag="PLB25_16685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HOE43244.1"
                     /translation="SLIYFLIILLMCFILYNWMQRVGTQAKEGGHE"
BASE COUNT          986 a         1205 c         1034 g          926 t
ORIGIN      
        1 aaatgatcct cacaaaccag gccgctcacc ttgccgttgt cataggtaag ctctatagcg
       61 cggcaataat tgatcatcaa ggcaccacgt gcggctgcgg ttcgggccaa agccagggcc
      121 agccgggcat cattgaactg gccgtcccaa tacttgacac cacccttgag ccctcgtgct
      181 tgcaaggtag gcaagtattt caacgtctct gcacggctca gcagctcggt ttttcccaag
      241 ccggcgcgcc ctgccagggc atcgtacatt ttcagtccga taccgtaaaa gggtgcctcc
      301 caccagtggt aagttggcat gacaaaagtc tgcggttgcg ccagatacgg cgcattgttc
      361 aagattcgca tgcgttcctg caaggcttcc tgcactaggg aaatattgcc ctgcgccaga
      421 tatcgaaccc cgccatgcac cagtttggtg gaacgtgacg aggtgccttt ggcaaaatca
      481 tgcgagtcaa tcaacaccac ccgcagcccg cgcaccactg catccagtgc gacacccaaa
      541 cccgtcgcgc caccgcccac aacggcaata tcgtattgag ttggcttggc caaatctgac
      601 atcaaatcgg cgcggcgggt tgccaatggc aatgcgatct tggtagtcat attatttaag
      661 gcttacgttt tgaatcactt tttttagctt gccgggccaa ccttggtgag tattcaacct
      721 agattcgata aaactgtagc acgctacgct ttcaggacaa gggctacagg ccaattcaat
      781 gttcctttgc gtggacatcc aggcaaccaa gagacagcag ccggtcggac cgatggcgca
      841 ctggctgcga accgactgca ccggccaagt tcgcgccgat cttttgactc acagccgggc
      901 tgcaggcctg caaatcaggc acaaaagggg cttcgctggg gtcgattttc gctttcagtg
      961 ccataacgcc gacagactcc tggctgcttt ggttacccca aggggaaaaa gatgaccgtg
     1021 cggcatgact actcacacgg ccaacgatca ccacaaccaa aatatcaggt cgcagtcaac
     1081 gaaccttgcc gttcttccac gcactcagca atgtttcata ggcaatcgtc tcacctttgg
     1141 gcttttcatt ggccaacttc ttccagggtg ctcccttgtc actgagccac ttgcccggat
     1201 cgcccttcgc gttgagcttg ggtgcacaaa ctgccatgcc tgaacgctcc agtctggcca
     1261 tcacgtcatc catctcggtg gccaaattgt ccatggccgc ttgaggtgtc ttttcgccag
     1321 tcactgctac ggccacgttt ttccaccaca gttgcgccag tttcggatag tcgggcacgt
     1381 tggtaccagt tggggtccat gccactcgcg ccggactgcg gtaaaactcg acaagaccgc
     1441 cgagtttcgg tgccatgtca gtcatcgcct tggactgaat gtcagactct cgaattggtg
     1501 tcagacccac aatcgtcttc ttcaatgacg tggacttggc cgtcacgaat tgagcgtata
     1561 gccacgctgc agccaccttg ttggcatcat ggtccttgaa aaaggtccag gaaccgacgt
     1621 cctggtaacc gttttgcatg ccctgtttcc agtatggccc gttgggacct ggtgccatgc
     1681 gccactttgg cgttccatca gcgttgacca ccggaagtcc gggtttgatc atgtcagcgg
     1741 tgaatgctgt gtaccagaaa atctgctggg caatttgacc ttgggccggt accggtcctg
     1801 cctcaccgaa agtcatgccg atggcttcct tgggtgagta ttttttcatc cagtcaatgt
     1861 acttggtcaa cgcgtaaacc gcagcaggtg agttggttgc accaccgcgc gcggttgaag
     1921 cgccctgcgg attgcaaccg tcagccgaag cgcggatgcc ccattcatcc accggcttac
     1981 cgttggggat gccaatatcg gccgtacctg ccatggaaag ccaggcatcc gtaaagcgcc
     2041 agcccagcga cggatctttc ttgccgtagt ccatgtgtcc atagatcggc ttgccgtcga
     2101 tcgttttcac gtcttcactg aaaaacgcag caatgtcttc gtaggcactc cagttgagcg
     2161 ggacacccag gtcatagcca tatttgacct tgaacttagc tttcaaatcc tgacgtgcaa
     2221 acaagtcagc ccgaaaccaa tacaggttgg caaactgctg gtcaggcagt tggtacattt
     2281 ttttgtctgg cgcagtggta aagctggttc cgatgaagtc tttcaggtca aggcccgggt
     2341 ttgtccattc tttaccacta ccgctcatgt agtcggtcag gttcaagacc ttgccgtacc
     2401 ggtagtgggt accaatcagg tcagagtcgg tgatccatcc gtcgtaaatc gacttgcctg
     2461 attgcatcga ggtttgcagc ttttcaacca cgtcaccttc ctggatcaaa tcgtgtttga
     2521 ccacaattcc agtgatttcg gtgaaggcct tggccagcgt ttttgattcg tactcgtgag
     2581 tcgtgatcgt ctcggacacg acagaaatct ctttcacgcc tttggcttgc agctttttgg
     2641 ccgcatcaat gaaccacttc atctccgcag tttgttgatc cttggacagc gttgagggct
     2701 gaaattcgct atcgatccac tttttggctg cagcctcatc ggcccaaccc tgactggcca
     2761 gcgcgcaagc aatggcaacc gacaatgccg tgtaccgcaa cttcatggta aatctcctct
     2821 aagaatgatg cccccgggct taaaggactc ttcggctccg ggggacggtg agccggtcct
     2881 tgaaaactca acgctatcct ttgcgcatga tcagcgccag caaacccatc gacaatacaa
     2941 aactgatcca gatcgagggc tctgactcca gttccatcca gaccaccagc ttgccactga
     3001 tgcccacaaa agccagattc acataagccg ctgtcagcag gcctatgaac aatcggtcgc
     3061 cccgggtggt ctcaagcggc agaaagccgc gacgcaaact cgtgggtgca ttgatttccc
     3121 acaccgtcat gccaaacagc atcagcccaa tgcacacaaa aaacaccgcc acgggcgtgg
     3181 tccaagccat ccattcaaac attcaacacc ccttcattcg gttgtgatcg gcacttggca
     3241 aggcatcaca cgcgtcccat cgcaaacccc ttggcgatgt agttgcggac aaaccagatc
     3301 acgatggcac ccggcacaat cgtcagaaca ccagcggccg cgagagttgc ccagtccatg
     3361 ccggacgctg aaacggtgcg cgtcatcgtc gccacgatcg gcttggcatt cacgctggtc
     3421 agcgtgcgag ccagcaacag ttccacccaa ctgaacatga agcaaaagaa agcggccaca
     3481 ccaacacccg cctttatcaa cggaatgaat atcctgatga aaaactttgg aaacgaataa
     3541 ccgtcgatat atgccgtctc gtcgatctcg cgcggtatgc cgctcatgaa cccttccaga
     3601 atccagactg ccagcgggac gttgaataac aggtgcgcca gcgcaacggc cagatgcgtg
     3661 tccatcaacc cgaccgtggt gtaaagctga aagaatggca acaaaaatac cgcaggcggt
     3721 gtcatgcggt tggtcagaag ccagaaaaac acatgcttgt cgcccagaaa actgtagcgt
     3781 gaaaaggcat aggcagcggg caaggcgact gtgattgaaa tcaccgtgtt gatgccgaca
     3841 taaatcagac tgttgatata ccccgagtac cacgacacat cggtcaggat cgttcggtag
     3901 ttggcccagg taaagtgctg cggaaacaac gagaaactgg ccagaatttc atcgttcgtc
     3961 ttgaacgaca tgttgaccat ccagtacacc ggcagcaagg caaacacaat gtaagccaac
     4021 aaaaaaatgg ttcgcttctg aaagcccgga ctattcatgg ccaccctcct tcgcctgcgt
     4081 gcccacacgc tgcatccagt tgtagaggat gaagcacatc agcaagatga tcagaaaata
     4141 gatcaaggaa a
//
LOCUS       DAOSLL010000445         4143 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19002_length_4143_cov_4.005626, whole genome shotgun sequence.
ACCESSION   DAOSLL010000445 DAOSLL010000000
VERSION     DAOSLL010000445.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19002_length_4143_cov_4.005626"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            87..746
                     /gene="pth"
                     /locus_tag="PLB25_16690"
     CDS             87..746
                     /gene="pth"
                     /locus_tag="PLB25_16690"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HOE43245.1"
                     /translation="MIRLFAGLGNPGPDYENTRHNAGFWWVEAVARSFNVPLVMEKAY
                     FGRVGRSTHLGKTIWLLEPQTFMNLSGKSVAALANFFKIAPQEILVAHDDLDIAPGEA
                     KLKLGGGHAGHNGLRDIHAQFGNDAYWRLRLGIGHPGNKAEVIHWVLKKPSPDHRIAV
                     DQSIDRALTALPHLLDGDMDQATRLIHTSKPPRPKPPRPSPVAEQATSSGPPGPAPAT
                     S"
     gene            755..1192
                     /locus_tag="PLB25_16695"
     CDS             755..1192
                     /locus_tag="PLB25_16695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43246.1"
                     /translation="MTTILFIAISAVCTGSGAETIYKCGTSYSQQPCAAAAALNLADE
                     RTPAQKTQADEATRRDRQLADTLRKERLTDEKLALAAQRESAKKGATSKTVTPPAQPK
                     ARVVTIKTKPVKISKPTAVAAKVPTSEAKAAVKKAATTKTSKD"
     gene            complement(1213..1527)
                     /locus_tag="PLB25_16700"
     CDS             complement(1213..1527)
                     /locus_tag="PLB25_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HOE43247.1"
                     /translation="MALLITDECINCDVCEPECPNDAIRMGEEIYLIEPQKCTECVGH
                     FDEPQCVQVCPVNCIPVNPQWLESRDTLWKKYRLLQSQAAVASVGNRAGACDLGALPP
                     VA"
     gene            complement(1549..2004)
                     /locus_tag="PLB25_16705"
     CDS             complement(1549..2004)
                     /locus_tag="PLB25_16705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOE43248.1"
                     /translation="MKILLPFDGSATALDAVRFAISLAASGLETSVVLANVQEPASLY
                     ELMVAHDPRVIEQVSAAAGANTLLPAQALLEAAGIGFETEVAMGDPAHTLVDILENYG
                     CDLVVMGASGTSTLRSALLGSVSNEVLHAAGVPVMIVKGGDSSNPDIAE"
     gene            complement(2025..2525)
                     /gene="coaD"
                     /locus_tag="PLB25_16710"
     CDS             complement(2025..2525)
                     /gene="coaD"
                     /locus_tag="PLB25_16710"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HOE43249.1"
                     /translation="MPNDLIAVYPGTFDPITLGHEDMVRRAAQLFGRVIVAVAAGHHK
                     KALFSTQDRVDMARNALRAHEGVEVAAFDGLLRDFVLARNVRAMVRGLRAVTDFDYEF
                     QLAGMNRCLMPEVETVFLAPNDKYQFISSTLIREIAILGGEVEKFVSPGVNLLLLEKV
                     RSMAKA"
     gene            2666..3247
                     /locus_tag="PLB25_16715"
     CDS             2666..3247
                     /locus_tag="PLB25_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HOE43250.1"
                     /translation="MNIDPATVQDVSYRETGQAQAPIRPTFHFMMARPAHVMALGFGA
                     GLSPKAPGTMGTLWAWVVFAMLQQRLTEVQWGVLIGISTLAGWWVCTVTARNLRLPDP
                     RCVVWDEIAAFWLVLWLINPGSFWSQLVAFVLFRFFDAVKPGPVAWADQLFHGVDPAQ
                     DRRAWHKAGLGIMLDDYMAAFCTLLVLALWRVL"
     gene            3265..3762
                     /locus_tag="PLB25_16720"
     CDS             3265..3762
                     /locus_tag="PLB25_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HOE43251.1"
                     /translation="MLLNKELSAQSASGLVADLIVKKGWMLATAESCTGGMIAAACTD
                     LAGSSAWFERGFVSYSNAAKTDLLGVPTDLIEHHGAVSEAVVRAMAAGALTRSNAQVA
                     LAVTGVAGPTGGSPAKPVGTVWFGWASPGGLISEVQQFAGDRAAVRLATVQHALVGLL
                     RCLGG"
BASE COUNT          878 a         1273 c         1203 g          789 t
ORIGIN      
        1 gcgggcaggt ttggagccgc ggctttcgcc ctacccagac gcgccgccag cggcctacac
       61 gggcgtgtcc aactgacgaa tccaggatga tccgactctt tgcaggcttg ggaaaccccg
      121 gccccgacta tgaaaacacg cgccacaacg ccggtttttg gtgggttgaa gcagtcgccc
      181 gcagcttcaa cgtgccattg gtcatggaga aagcctactt tggacgggtc ggccgcagca
      241 cccatcttgg caagaccatc tggctactcg agccccagac cttcatgaat ctgagcggca
      301 aatcggttgc cgcgttggca aactttttca aaatagcacc gcaggaaatt ctggtggccc
      361 acgacgatct tgacattgcg ccgggcgaag ccaaactcaa gctcggtggc gggcatgccg
      421 gccacaatgg gctgcgcgac attcatgccc aattcggcaa cgacgcttac tggcgcttgc
      481 ggcttggcat tggccacccg ggcaacaagg ctgaagtgat tcactgggtg ctgaaaaagc
      541 catctccgga tcaccgcata gccgtcgatc aaagcatcga ccgggctctc accgcactgc
      601 cgcacctcct ggacggcgac atggaccaag ccacccggct gatccacacc agcaagccgc
      661 cacgccccaa accacccaga ccgagcccgg tggccgagca ggcaaccagc tccggaccac
      721 caggcccggc accagcaaca tcatgaaacg acacttgacg actatattat ttatagctat
      781 cagcgcagtc tgcacgggct ctggagctga aaccatctat aaatgcggaa ccagctacag
      841 tcagcagccc tgcgctgcgg cggcggcact caatctcgca gatgaaagaa caccggccca
      901 gaaaacgcag gctgacgagg cgacccgtcg cgaccggcaa ctggccgata ccctgagaaa
      961 agagcgcttg actgacgaga aattggcact tgcggcgcaa cgcgaatcgg ccaaaaaagg
     1021 tgcgacatca aagacagtca cgccaccggc ccagcccaag gccagggttg tgaccatcaa
     1081 aaccaaaccc gtcaaaatca gcaagccgac tgctgttgcg gcgaaagtgc cgacttccga
     1141 agcgaaggct gcggttaaaa aagctgccac gaccaaaacc agcaaagact gagccaacca
     1201 gcccagccga attcaggcca ctggcggcaa tgcgcccagg tcgcaagcgc ctgccctgtt
     1261 gccaactgaa gcaacagcgg cttgcgattg caacagccgg tattttttcc agagcgtgtc
     1321 ccggctctca agccactgcg gattgaccgg aatgcagttc accggacata cctgaacaca
     1381 ctggggttca tcgaaatggc cgacacattc ggtacatttt tgtggctcaa tcaggtagat
     1441 ttcctctccc atgcgaatcg catcattcgg gcattctggc tcgcagacat cgcaattgat
     1501 gcattcgtca gtgatgagca aagccatgat ggagcgccgc cgcctgatct attcggcaat
     1561 gtcagggttg ctgctgtcac cgcccttgac gatcatcacc ggcacgccag cggcgtgcag
     1621 cacttcattt gacaccgacc ccaacagcgc gctgcgcagc gtgctggtgc cgctggcccc
     1681 catcaccacc aggtcgcaac cgtagttctc aagaatgtcc accagcgtgt gcgccgggtc
     1741 gcccatggca acctcggttt caaagccaat accagccgct tccagcagcg cctgggccgg
     1801 cagcagggta ttggcaccgg cggcggcgct gacctgctca atcacgcgcg ggtcatgcgc
     1861 caccatcaat tcgtacaggc tggcgggctc ctgcacgttg gccagcacca cgctggtttc
     1921 aagcccgctg gctgccaggc tgatggcaaa gcgaaccgca tccagcgcag tggcagaacc
     1981 atcgaagggc aaaaggatct tcatcgttac tccaatcagg ccggtcaggc tttggccatg
     2041 ctgcgaactt tttcaagcaa cagcaggttc acgccaggcg atacaaactt ttccacctcg
     2101 ccacccagga tggcgatctc acgaatcagc gtgctggaaa tgaactggta cttgtcattg
     2161 ggtgccagaa agacggtttc cacctccggc atcaggcatc ggttcatgcc tgcgagctga
     2221 aactcgtagt caaaatcagt gacagcgcgc aagcctcgca ccattgccct gacattcctg
     2281 gccagcacaa aatcgcgcag caagccgtcg aaagcggcca cctccacccc ctcatgggcg
     2341 cgcagcgcat tacgcgccat atcgacccgg tcctgggttg aaaaaagggc ctttttatgg
     2401 tgtccggcgg ccaccgccac aatcacccgg ccaaacagtt gtgcagcgcg gcgaaccatg
     2461 tcttcatgcc ccagggttat cgggtcaaag gtgccggggt ataccgctat caaatcgttg
     2521 ggcatattga aaatctggat tatttgaaac acgccgatta tcaacgccga catccttgac
     2581 gcgcctgggc tcacttttaa agaccgcccg gctggtggcg cggcgctggc gctgatgcca
     2641 tgctgccaaa atggataatc aggcgatgaa cattgacccc gcaacggtgc aggatgtttc
     2701 ctatcgcgaa actggccagg cacaggcacc catccgacca acttttcatt tcatgatggc
     2761 acgtccggcg catgtgatgg cgctcgggtt tggtgctggc ctgagcccaa aagcacccgg
     2821 caccatgggc acgctgtggg cctgggtggt gtttgccatg ctgcaacagc gcctgaccga
     2881 ggtgcaatgg ggcgtgctga ttggcatcag caccctggca ggctggtggg tctgcacggt
     2941 aaccgccaga aacctgcgcc tgccagaccc gcgctgcgtg gtgtgggacg aaattgccgc
     3001 cttctggctg gtgttgtggc tgatcaaccc gggcagcttt tggagccagt tggtggcgtt
     3061 tgtgctgttt cggttttttg atgcggtcaa acccggcccg gtggcctggg ctgaccagtt
     3121 gtttcatggg gtcgatccgg cgcaggaccg ccgcgcctgg cacaaggccg ggcttggcat
     3181 catgctcgat gactatatgg ccgcattttg cacgctgctg gtgctggcgc tgtggcgggt
     3241 tttgtgatca ccgggaacac ctttatgcta ctcaataaag agctgtcagc gcagtctgca
     3301 tctgggctgg tggccgattt gattgtcaaa aagggctgga tgctggcgac cgccgagagt
     3361 tgcaccggcg gcatgattgc agccgcctgc accgaccttg ccgggtcaag cgcctggttt
     3421 gagcgcggct ttgtcagcta ttccaacgcc gccaagaccg acttgctcgg tgtgccgacg
     3481 gacttgattg aacaccatgg cgcggtcagc gaagcggtgg tgcgcgccat ggcagcgggt
     3541 gcgctgaccc gctcaaacgc ccaggtagcg ctggctgtga ccggggtcgc tgggccgact
     3601 ggtggcagcc cggccaagcc ggtcggcaca gtctggtttg gctgggccag cccgggcggc
     3661 ctgatcagcg aggtgcaaca gtttgccggt gaccgcgctg cggtgcgcct ggccacggtg
     3721 caacatgcgc tggtcgggct gctgcgttgc ctgggtggct gaatctgcct gcatttgcga
     3781 ctggcaattt ggtgccgaat catcagcagc accatgggca tggcgtgctt tgcctgcctc
     3841 gacatgcccg aacgatcggg caatgctggt ctgacggtgg aaactagccg aaccaggcgc
     3901 agacaatgcc tggcaagctg acacaagcca caaaattgtg gtcaatttgc cttcaggcgc
     3961 ttatccagca tgcgtaacta gctattaata gtatagtatt tcaatgattc tgaaagtgac
     4021 cgcgtcgttg cagccgatca gcacattgcg cccagaccgc tggtcaatcg tgggctggtc
     4081 ccgccgcaac aaggcgattc gggagagcat ccgcccagcc tggggggctc cttctgccac
     4141 tcg
//
LOCUS       DAOSLL010000446         4119 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19127_length_4119_cov_4.239911, whole genome shotgun sequence.
ACCESSION   DAOSLL010000446 DAOSLL010000000
VERSION     DAOSLL010000446.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19127_length_4119_cov_4.239911"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1060
                     /locus_tag="PLB25_16725"
     CDS             <1..1060
                     /locus_tag="PLB25_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43252.1"
                     /translation="LNNTARHANSHLAIMGKPVVGKTQFLLKILTDIRIQSNYQTNFI
                     YFDYKGDVVENARFLELARVQPFRLLQGGQSLPINPFILPAYDEQTINVSAREKAESF
                     ASINSKLGVVQKGALSEAIRAAYVKRAGSATPYPDFPDILEAANVAYEADGKKDDSLI
                     EVLRDLSDFDLFWRHGTEGTPMDRVANRTMLIDVHAMPVLKELVAYLVIERLYKEMAT
                     LPDSPVTEGRRTIRTILVIDEAHNYLGQRNMFLQRIIREGRSKGVVVFFASQSPNDYQ
                     QKFFNFQELLEFAYIFQCDGVAGGSVQDILGCSAKTAKELQTEIARLEPWQVIARSQA
                     KTEDYVKFTAEAFYKSYA"
     gene            complement(1151..2617)
                     /locus_tag="PLB25_16730"
     CDS             complement(1151..2617)
                     /locus_tag="PLB25_16730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HOE43253.1"
                     /translation="MGKITGFMEIERLEEGYKPVPERLKNYQEFVIALDDTQSRQQAA
                     RCMDCGTPFCNSGCPVNNIIPDFNDLVFQGDWKNAIDVLHSTNNFPEFTGRICPAPCE
                     AACTLNVNQLPVGIKSIEHAIIDRAWTEGWVKPQPARHKTGKKVAVVGSGPAGMAAAQ
                     QLARVGHAVTLFEKNDRVGGLLRYGIPDFKMEKSHIDRRVEQLKAEGVTIRTGVLVGA
                     MPKGSKVTNWAKETLSPEQLNQEFDAVLLTGGSEQSRDLPVPGRDLDGIHFAMEFLPQ
                     QNKVNAGDKLKGQLRADGKNVIVIGGGDTGSDCVGTSNRHGAVSVTQFEVMPQPPVEE
                     DRPMTWPYWPMKLRTSSSHDEGCTREFAISTQEFIGEKGKVTGLKTVQIEFKDGKMVE
                     VAGTAKTYKADLVLLAMGFVNPVATVLEAFGIEKDARGNAKASTDFTGGYATNVPKVF
                     AAGDMRRGQSLVVWAIREGRQAARAIDEFLMGFSDLPR"
     gene            complement(2750..>4119)
                     /locus_tag="PLB25_16735"
     CDS             complement(2750..>4119)
                     /locus_tag="PLB25_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HOE43254.1"
                     /translation="VLNRLRSRIRVQTDGQMKTGRDVAIGALLGADEFGFATAPLVVE
                     GCIMMRKCHLNTCPVGVATQDPVLRQKFSGKPEHVVNYFFFIAEEVRQIMAQLGIARF
                     DDMIGRADLLDMRAGINHWKAKGLDFSRLLAMPQVAADVPRFHQEQQDHGLDKALDNV
                     LIAKSRAAIDKGERVQFIEVARNVNRSVGAMLSGALTQVHPEGLPDDSIRIQLEGTGG
                     QSFGAFLARGITLYLIGDANDYTGKGLSGGRVVVRPSIDFRGEATKNTIVGNTVMFGA
                     TSGEAFLSGVAGERFAVRLSGATAVVEGTGDHGCEYMTGGTVVVLGKTGRNFAAGMSG
                     GIAYVYDEDGQFAKRCNTAMVALENVLSVVEQQASSNEKLWHRGLADEVQLKKLLEEH
                     NRWTGSKRARELLDNWQVSRQKFVKVFPHEYKRALGEIHAKKSALALVGLAQAAIKKA
                     AASVS"
BASE COUNT          800 a         1246 c         1191 g          882 t
ORIGIN      
        1 gctgaataac accgctcgcc atgccaactc gcacctggcc atcatgggta agccggttgt
       61 cggcaagacc cagtttttgt tgaagatact gaccgacatc cgcattcagt cgaattacca
      121 gaccaacttc atttattttg actacaaggg cgacgtggtg gaaaacgccc gttttctgga
      181 actggcgcgg gtgcagccgt ttcgcctgct gcaaggcggg cagagcctgc cgatcaatcc
      241 gttcatcttg ccagcctatg acgagcagac catcaacgtg tcggcgcgtg aaaaggccga
      301 gagctttgcg tcgatcaaca gcaagcttgg ggtggtgcaa aagggcgcat tgagcgaagc
      361 gattcgcgcc gcctatgtta agcgtgctgg ctcggccacg ccttaccctg actttccgga
      421 cattcttgaa gctgccaacg tggcttatga ggccgatgga aagaaagacg acagcctgat
      481 tgaagtgctg cgcgacttgt ctgactttga tctattttgg cgccacggaa ccgaaggtac
      541 gccgatggat cgggtggcga accggaccat gttgattgat gtgcatgcta tgccggtgct
      601 caaagagctg gtggcctatc tggtgattga gcggctttac aaggaaatgg cgacactgcc
      661 cgacagtccg gtaacggaag gccgcaggac cattcggacc attttggtca ttgacgaggc
      721 tcacaactac cttggacaga gaaacatgtt tttgcaacgc atcatccgcg aagggcgatc
      781 caaaggcgtg gtggtgttct ttgccagcca gtcccccaac gattaccagc agaaattttt
      841 caattttcag gaactgctgg agtttgcgta catctttcag tgcgatggcg tggcaggcgg
      901 atcggtgcag gatattctgg gttgcagcgc caagacggcg aaggaattgc agaccgaaat
      961 cgcgcggctg gaaccctggc aagtgattgc ccgcagccaa gccaagaccg aggattatgt
     1021 gaagttcacg gcggaggctt tttacaagag ctacgcgtga tttggccgcc ttgagccggt
     1081 agtcttgggc aatcaaagca gcccaaccgc agcaatctga tgatgcggca gacctcttgc
     1141 catgaatcga tcaacgcggc agatcgctga aacccatcag aaactcatcg atagcacgcg
     1201 ccgcctggcg accttcgcgg atggcccaca ccaccaggct ctggccgcgg cgcatgtcac
     1261 ccgcagcaaa caccttgggc acgttggtgg catagccgcc ggtgaaatcg gtgctggctt
     1321 tggcattgcc acgggcgtcc ttctcgatgc caaaggcttc cagcacggtg gccaccgggt
     1381 tgacaaaacc catggccagc agcaccagat cggccttgta ggtctttgcg gtgccagcga
     1441 cctccaccat ttttccgtcc ttgaattcga tttgcacagt cttcagaccc gtgaccttgc
     1501 ccttttcacc gatgaattcc tgggtggaaa tggcaaattc gcgggtgcag ccctcgtcgt
     1561 ggctggaact ggtgcgcagt ttcatcggcc agtacggcca ggtcatcggg cggtcttctt
     1621 ccaccggggg ctgcggcatc acctcaaact gggtgacact cactgcaccg tggcggttgc
     1681 tggtgccgac acagtcgctg ccggtgtcac cgccgccaat cacgatcaca tttttgccat
     1741 cggcgcgcaa ctggcccttg agcttgtcgc ccgcgttgac cttgttttgc tggggcaaaa
     1801 actccatggc aaagtgaatg ccgtccagat cacggcccgg caccggcagg tcacgcgact
     1861 gctctgagcc accggtgagc agcaccgcgt caaactcctg gttgagttgc tcgggcgaca
     1921 gtgtttcttt ggcccagttg gtcaccttgc tgcccttggg catggcaccg accagcacgc
     1981 cggtgcgaat cgtcacaccc tcggccttga gttgctcgac gcggcggtca atgtgcgact
     2041 tttccatctt gaagtcggga atgccgtagc gcagcaggcc gccgacccgg tcgttcttct
     2101 cgaacaatgt cactgcgtga ccaacccgcg cgagttgctg cgccgccgcc atgccggccg
     2161 ggccggaacc taccaccgcc accttcttgc cggttttgtg cctggctggc tgcggcttga
     2221 cccagccctc ggtccaggcg cggtcgatga tggcgtgctc gatgctcttg atgcccaccg
     2281 gcaactggtt gacgttgagt gtgcaggctg cttcgcaagg tgccgggcag atgcgcccgg
     2341 tgaactcggg aaaattgttg gtgctgtgca gcacatcgat ggcgtttttc cagtcgccct
     2401 ggaagaccag gtcattgaaa tccggaatga tgttgttgac cgggcagccg ctgttgcaaa
     2461 acggtgtgcc gcagtccatg cagcgagccg cctgctgcct ggactgtgtg tcgtcgagcg
     2521 cgatgacaaa ctcttggtaa tttttcagac gctcggggac cggcttgtaa ccctcttcca
     2581 gacgctcaat ctccatgaaa cctgtgattt ttcccatgat gtattccttt gccgggcaga
     2641 cctttcgccc tgcccgcact actttttgat tcgtcattgc cactttggcg cgatgctggc
     2701 ggcactggat gcccgcctgc gcggcaatga cggcgtaaca aacttcacct caagacactg
     2761 aagctgctgc tttttttata gctgcttgcg caagtccgac aagggctaga gccgatttct
     2821 ttgcatgaat ttcccccaag gcgcgtttgt attcatgggg aaaaaccttg acgaatttct
     2881 ggcggctaac ctgccagttg tccagcagtt cgcgcgcgcg cttgctgccg gtccagcggt
     2941 tgtgctcttc gagcagtttc ttgagctgca cttcatcagc cagcccgcgg tgccagagct
     3001 tttcgttgct gctggcctgc tgctcaacca ccgacagcac gttttccagc gccaccatgg
     3061 cggtattgca gcgcttggca aactggccgt cttcgtcata aacgtaggca atgccgccgc
     3121 tcatgccagc ggcaaagttg cgcccggtct tgcccagcac caccaccgtg ccgccggtca
     3181 tgtattcgca gccgtggtca ccggttccct cgacgactgc ggtggcacct gacagacgca
     3241 cggcaaaacg ctcgccggca acgccactta aaaaggcttc gccgctggtg gcaccaaaca
     3301 tcacggtgtt acccacaatg gtgttcttgg tcgcctcacc gcggaaatca atgctgggcc
     3361 gcaccaccac acggccgccc gacagaccct tgccggtgta atcgttggca tcgccaatca
     3421 agtacagggt gataccacgc gccagaaagg caccaaacga ctggccaccg gtaccctcca
     3481 actggatgcg aatgctgtcg tcgggcaagc cttccggatg cacctgcgtc agggcaccgg
     3541 acaacatggc cccgaccgag cggttgacat tgcgcgccac ctcgatgaac tggacccgct
     3601 cgcccttgtc gatggccgcc cgcgacttgg caatcagcac gttgtcaagc gctttgtcca
     3661 gcccgtggtc ttgttgctcc tggtgaaacc tgggcacgtc agcggccacc tggggcatcg
     3721 ccagcagacg gctgaagtcc agccccttgg ccttccagtg gttgatgcca gcgcgcatgt
     3781 cgagcaggtc agcgcggcca atcatgtcgt caaacctggc gatgccgagc tgcgccatga
     3841 tctggcgtac ctcttcagcg atgaaaaaga agtagttgac cacatgctcg ggcttgccgg
     3901 agaacttctg gcgcagcacc gggtcttgtg tggcgacccc gaccgggcag gtgttgaggt
     3961 ggcacttgcg catcatgatg cagccttcga caaccagcgg cgcggtggca aaaccaaact
     4021 cgtcggcacc gagcagcgcc ccgatcgcca cgtcgcggcc ggttttcatc tggccgtcgg
     4081 tctgcacgcg gatgcggctg cgcaggcggt tgagcacca
//
LOCUS       DAOSLL010000447         4117 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19135_length_4117_cov_5.165190, whole genome shotgun sequence.
ACCESSION   DAOSLL010000447 DAOSLL010000000
VERSION     DAOSLL010000447.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19135_length_4117_cov_5.165190"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="PLB25_16740"
     CDS             <1..224
                     /locus_tag="PLB25_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017777062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HOE43255.1"
                     /translation="TRLGNDIGQGCAPQHVVRANTWFAARVAAGGEFALVGCSVAPGF
                     DFADFELAQRIALMAEFPQHRHVIAGLTR"
     gene            complement(422..1186)
                     /locus_tag="PLB25_16745"
     CDS             complement(422..1186)
                     /locus_tag="PLB25_16745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOE43256.1"
                     /translation="MTLLATFPLITDPYFYAVSIPAVLLMGISKSGFGAGFGSLAVPM
                     MALAVTVPQAAAILMPLLFVMDVLGMAAFRKDFDLKLLGFLIPCGLVGIVVGALLFKM
                     LNAHTVAGIVGGFTLLFLAQRLLFAPKADSRAPPRWLGAMLTATSGFTSFIAHAGGPP
                     INAYVIPMRLSPVRFTATMAFFFFVINLAKWIPYAWLGLLDWRNLATSLVLLPVAPLG
                     VWVGVRLARRISQVLFYRFVYVGMFLTGVKLLWDAF"
     gene            1207..1641
                     /locus_tag="PLB25_16750"
     CDS             1207..1641
                     /locus_tag="PLB25_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HOE43257.1"
                     /translation="MFSPSQADVRRFFCSIYAKAQTGQALEAIEKIASLWIDEHPEYH
                     AELADQDAALANMAKPEDGNSNAFLHLSMHLSISEQCSIDQPRGIRQATELLAHRRGS
                     LHDAHHEVMACLGNMIWESQRSGRPPDGNTYVACVQRRATRD"
     gene            complement(1651..2310)
                     /locus_tag="PLB25_16755"
     CDS             complement(1651..2310)
                     /locus_tag="PLB25_16755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HOE43258.1"
                     /translation="MKKLLFTVPTLLIALASVLPVQAQDIKGDAQAGSHKNALCVGCH
                     GIKGFHTAFPATYRVPKLSGQGAGYLSAALHAYKSGERKHPSMRTLAASLSEQDIADL
                     SAYYESTGTGATLAPGAEPGPAKVAELVTKGACASCHGENFSKPISPAYPRIAGQHSD
                     YLMAALRSYQTTDKPFIGRSNAIMGGVAKQFSLADLKLLADYMGSLPGELQTVQPVRF
                     K"
     gene            2479..3333
                     /locus_tag="PLB25_16760"
     CDS             2479..3333
                     /locus_tag="PLB25_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HOE43259.1"
                     /translation="MKFQGTQNYVATADLMLAVNASITLQRPLLVKGEPGTGKTMLAE
                     EVAQALNLPLLQWHIKSTTKAQQGLYEYDAVSRLRDSQLSDLDGGERVKNIHNYIVKG
                     VLWQAFTAEQPVALLIDEIDKADIEFPNDLLREIDRMEFYCYETRELIRAKHRPLVFI
                     TSNNEKELPDAFLRRCFFHYIKFPDAPTMTRIVDVHFPGLKAELLSAAMKTFFDVRNL
                     PGLKKKPSTSEFLDWLKLLLAQDIPASVLQTADDKVAVPPLVGALLKNEQDVTLFEKL
                     VFMQSRNR"
     gene            3595..>4117
                     /locus_tag="PLB25_16765"
     CDS             3595..>4117
                     /locus_tag="PLB25_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE43260.1"
                     /translation="MAFIEPVTLATRDIVLLPLALEHEEGLRCAAADGELWNIRVTSV
                     PEPGQTRKYIEDALVMREAGTRLAFAVTNAATGKVLGSSSFHDILPAVKRVEIGYTWY
                     AKSAQRSHVNTTTKLLMMSHAFEVLGCHVVGWRTDNFNYASQAAIERLGAKKDGVIRG
                     HALRRDGTIRDTVM"
BASE COUNT          910 a         1214 c         1229 g          764 t
ORIGIN      
        1 gtacccgttt gggcaacgat atcgggcaag gctgtgcgcc gcagcatgtg gttcgggcca
       61 acacctggtt tgccgccaga gtggcagcgg gcggcgagtt tgcgctggtt ggttgcagcg
      121 tggcaccggg ctttgacttc gccgattttg aactggcgca gcgaattgcc ctgatggctg
      181 agtttcccca gcaccgtcac gtcattgccg ggctgacccg atagcgggtt ggcggcaatc
      241 ggcggcgcag ggtgcgccag tcaaccggtg gccatgctgc aaggcaggcc aatgcggctt
      301 accggggcaa agcctgacac tgccaggccc gccgacagca ccaagtatga tgccaaatgt
      361 gcttccagac cttgtgctgt atgcgctggc agctatcttt aatgaagcaa tctgcagttc
      421 atcagaacgc gtcccacagc agcttgaccc cggtcagaaa catgccgaca tagacaaagc
      481 ggtaaaacag cacctgactg atgcgccgcg ccagccgcac gcctacccag acacccagcg
      541 gcgcgaccgg cagcagcacc agtgatgtgg ccaggttgcg ccaatcgagc aaacccagcc
      601 aggcgtaggg aatccacttg gccagattga tgacaaagaa gaaaaaggcc atggtggcgg
      661 taaaacgcac tggggagagc cgcatcggaa tcacgtaggc gttgatcggc ggcccacccg
      721 catgggcaat gaaactggtg aacccggagg tggcggtaag catcgcgccc agccatctgg
      781 gtggtgcccg gctgtcagcc ttgggcgcaa acagcaagcg ctgcgccaga aacagcagcg
      841 taaagccgcc cacaataccg gctacggtgt gggcattgag catcttgaac agcaaggcac
      901 ccaccacaat gccaaccaga ccacaaggaa tcaggaagcc cagcagcttg aggtcgaagt
      961 ccttgcgaaa cgccgccatg cccagcacgt ccatgacaaa cagcagcggc atcagaatcg
     1021 cggcggcctg cggcacggtc actgccagtg ccatcatcgg caccgccaat gagccaaaac
     1081 cggcaccaaa gccgcttttg ctgatgccca tcagcagcac ggccggtatg gagaccgcat
     1141 aaaaataggg gtcggtgatg aggggaaagg tggcaagcag cgtcatggcg cgacaattac
     1201 accaccatgt tcagtccatc ccaagccgat gtccgccgtt ttttttgctc gatttacgcc
     1261 aaagcccaga ccggacaagc gctggaagct attgaaaaaa tagcgtcgtt atggattgac
     1321 gaacatcccg aataccacgc cgaactggcc gaccaggacg ctgcactggc caacatggcc
     1381 aagcccgaag acggcaacag caacgctttt ttgcacctgt ccatgcacct gtcaatcagc
     1441 gagcaatgca gcattgacca gccgcgcggc attcgccagg cgaccgagtt gctggcacac
     1501 cggcgcggtt ccttgcatga cgctcaccac gaggtgatgg cgtgcctggg caatatgatc
     1561 tgggaaagtc agcgctcggg ccgcccaccg gacggcaaca cctatgtggc ctgtgtgcag
     1621 cggcgcgcca cgcgagactg aagacgctgc ttatttgaag cggaccggct gcacggtttg
     1681 cagttcaccg ggcagactgc ccatgtaatc tgccagcagt ttcaggtcgg ccagcgagaa
     1741 ctgtttggca acaccgccca tgatggcgtt actgcgacca atgaagggct tgtcggtggt
     1801 ctggtaggac cttagcgccg ccatcaggta gtcactgtgc tggccggcaa ttctggggta
     1861 agccgggctg atcggcttgc tgaagttttc gccatgacaa gaggcgcagg cacccttggt
     1921 caccagttcg gccactttgg ctgggccggg ctcggctccc ggggccagcg tggcaccggt
     1981 gccggtggat tcgtaatagg ccgacaggtc ggcaatgtcc tgctcactaa gggatgcggc
     2041 caacgtgcgc atggacgggt gtttgcgctc gccactcttg taggcgtgca aggcggcact
     2101 caaatatccg gccccttgcc cggacaactt gggcacccgg taagtcgctg ggaaggcggt
     2161 atgaaagccc ttgatgccgt ggcaaccgac acacagtgca tttttgtgcg acccggcctg
     2221 cgcatcgccc ttgatgtctt gtgcctgcac gggcaggacg ctggccaggg cgatcaacag
     2281 ggtcggcacg gtgaaaagta gtttcttcat gttgcgggaa cgatcgggtt ggaggtatgg
     2341 cgattgataa atatcaaata attataggga tcgagaatgc cacttcatca tggcgttaac
     2401 ccggtattga cgcagcatcc cggtgcatcc tggccagacc ctgtaagcta gcgcctttgc
     2461 acgactattc gacccaccat gaaatttcaa ggcacccaaa actacgtcgc aactgctgac
     2521 ctgatgctgg cagtcaatgc gtccatcacc ctgcagcgcc cgctgctggt caagggcgag
     2581 cccggcaccg gcaaaaccat gctggctgaa gaagtggcgc aggcattgaa cttgccgctg
     2641 ttgcagtggc acatcaagtc aaccaccaag gcgcagcagg gtttgtatga atacgatgcg
     2701 gtcagtcggc tgcgcgactc gcaactgtct gacctcgacg gtggtgagcg ggtcaagaac
     2761 atccataact acatcgtcaa aggggttttg tggcaagcct ttaccgccga gcaaccggtg
     2821 gcgctgctga tcgacgagat cgacaaggcc gacatcgagt tccccaatga cttgctgcgc
     2881 gaaatcgacc gcatggagtt ttattgctat gaaacccgtg aactcatccg cgccaaacat
     2941 cgcccgctgg tgttcatcac ctcgaataac gaaaaggaac tgcccgacgc gtttttgcgc
     3001 cgctgtttct tccactacat caagttcccg gatgcaccca ccatgacgag aatcgtggac
     3061 gtgcactttc ctggcctcaa ggctgagctg ctgagcgccg ccatgaaaac cttttttgac
     3121 gtgcgcaacc tgcccggact gaagaagaaa ccctctacca gcgagtttct ggactggttg
     3181 aagttgctgt tggcgcagga tattcctgca tcggtgctgc aaaccgccga tgacaaggtg
     3241 gcagttccgc cgctggtcgg tgccctgctg aaaaacgagc aggatgtcac gctgtttgaa
     3301 aaactggtgt tcatgcagag ccggaaccgg tgatttggct ggcagtcggc aagccgggag
     3361 ccagttgaac tgatggatcg agcggcaaca agcaaccctg gcaaggccag tgaggccatt
     3421 gaggccagtt tgtgccggat ttgcaggccg atagcccggt tgggggtcaa aaagtcagta
     3481 caaacagggt cgcttcggtc gcttcgctgc ctacttgcca acggtgcggt cggctggtgg
     3541 cacccgaccg ctctgggctg gctggcgaga ccaagaattc ttctggagaa cgcaatggca
     3601 tttattgaac ctgtcaccct ggcaacgcgc gacatcgtgc tgctgccgct ggcacttgag
     3661 cacgaagagg gcttgcgctg cgccgccgcc gacggcgaac tgtggaacat ccgcgtcacc
     3721 tcggtgcccg agcccggcca gacccgcaaa tacattgaag acgcactggt catgcgtgaa
     3781 gcaggcaccc gtcttgcctt cgccgtcacc aatgcggcga ccggcaaagt gctgggcagc
     3841 agcagtttcc acgacatcct gcccgccgtc aaacgtgtgg aaattggcta cacctggtat
     3901 gccaagagcg cccagcgcag tcatgtcaac accacaacca agctgctgat gatgagccac
     3961 gcctttgaag tgctgggttg ccatgtggtc ggctggcgca ccgacaactt caactatgcc
     4021 tcgcaagccg ccatcgagcg cctgggggcc aaaaaagacg gggtgatccg tggccatgcg
     4081 ctgcgccgcg acggcaccat ccgcgacacc gtgatgt
//
LOCUS       DAOSLL010000448         4110 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19174_length_4110_cov_3.957337, whole genome shotgun sequence.
ACCESSION   DAOSLL010000448 DAOSLL010000000
VERSION     DAOSLL010000448.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19174_length_4110_cov_3.957337"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="PLB25_16770"
     CDS             complement(<1..574)
                     /locus_tag="PLB25_16770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43261.1"
                     /translation="MAALGWLLALAHISVKQVKGFHQLATGNGLAVGAHGVAVIADLV
                     KPDPLSHLAFDKQQDRGFWPLAAFAHKQALRQADDGLQIAFLKQGTAQGAGAVLIPSQ
                     KALGHDDGANTTLLQGFQHQLDKQNGGLGLGGVFAPDIRVRLAVALRPKRRVCKDDGF
                     AARQNVGTEFLQRIALLNVAGVEAAHKEIDR"
     gene            656..2218
                     /locus_tag="PLB25_16775"
     CDS             656..2218
                     /locus_tag="PLB25_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43262.1"
                     /translation="MAAKEADDRAKQLEKWQAQVAAKKLTKAECTERLRQHAELWFGR
                     TENDIARKIERAHLYSFNLPNYRSSLSLAQIEAWESFAATRVREGKAATTLEAKYRAV
                     AAAGTDELDEMLSSLDPRHTMELDWARQFLAGVPNEEFGRHPHLNTLQEVIASQAKGA
                     RVQLKELIKPREEKIKKSAYDGVLPIVEKISFSDGRLHFREDRETGMDLYKACKGDLV
                     TSKINLHQGAVALAPSDLVTSTHYLPYEINVGEVNPEYLVVMIRSAQFRSLIDAQKNS
                     GIKNEQGAEFLGEFEIPIPDRKVQEELVAELKRCNKITDSSLTIVENWEAYIPPTDDR
                     RVIGEFVIESLYGIADKLTDHGDYPVLRMNNLDERGFWHLDDLKFIDRKPSEERLLKH
                     GDFIFNRTNSIALVGKSAVVDFDFPGTWAGYLVRLRFSDGLNPSYLRDVLSTRPFRQI
                     FAAIARPAGGQANLNVEEFSDISIPYFSPEKQVEIVAANQRDRLAIQQLDRIGQVARR
                     TSFQLLKKHWES"
     gene            2221..>4110
                     /locus_tag="PLB25_16780"
     CDS             2221..>4110
                     /locus_tag="PLB25_16780"
                     /inference="COORDINATES: protein motif:HMM:NF037510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOE43263.1"
                     /translation="MPKPIKTGVFDNEASTVDLKVYDTLKALDWKQGDTLLYQPSYAL
                     TEDEQKDYPGSKSIKPDFVLQDLQGFALAVIENKLDDPHKALPKLRLKYSRLLKPRFL
                     YACASDGAGGLKILFFDLAWRGVDAADFRVVDSFLPLADMKLKIEQDRLKRQELDIKI
                     DTTLAGGFDPAAGKERAYQLTCILTLLRLYREGKMKMLVHMATGLGKTRTMVALVKAL
                     LEHALAKRVLFVVDRRTLAHQAINKGFRLLAPSYNSHWITSSSWRAHKNKNIHVVVID
                     TLEQLYDKIPSNFYDLIIVDECHRSITVNRNLVFDHFVCPRIGLTATPRIAVAAKGGE
                     LNEDDAAINDTYRLFGCEVGDPDYKFDLDRAIDEGFLAPYRKEEHITALTKQAMETGV
                     LYDHLLDPETRKRIELGAQQTIELEKLNKRILSDEQANRWAEIIRKETEYGEKVLLFA
                     ASQAHSLMLVKAINKVFNDSGQSPRYAEAIISQNDDINESLKEWFERPYSNPRVVVSV
                     DIMSTGVDIPCLRYIAFGAITQSVGKYLQMLGRGTRLDPKTGKFSFKILDFVGLCKRM
                     DDNGKGTLMPNEKVVKPDAGGGGGGGGGGGAVDGILDNPDPASLIQRVLMTVDGIKII
                     DNIPIA"
BASE COUNT          998 a         1085 c         1128 g          899 t
ORIGIN      
        1 ctctatcaat ttctttgtgc gcggcctcaa ccccggcaac attcagcaag gcgattctct
       61 ggagaaattc ggtaccgaca ttctgccgcg cagcaaatcc atcgtccttg caaacccgcc
      121 ttttggggcg cagcgcgacc gcgagtctta cccgaatgtc tggagcgaat acgccaccga
      181 gtccgagacc accattctgt ttgtcaagct gatgttggaa accctggagc agcgtggtgt
      241 ttgcgccgtc atcgtgtccg agggcttttt gacttgggat caaaacagcg cccgcgccct
      301 gcgccgtgcc ctgcttgagg aatgcaatct gcaagccgtc atcagcttgc cgcagggctt
      361 gtttgtgagc aaaggcggcc aggggccaaa aacctcgatc ctgctgtttg tcaaaggcca
      421 agtgactgag cgggtctggt tttacaagat cagcaatgac ggctacacca tgggcaccaa
      481 ccgcaagccc attgccggtt gccaattggt ggaagccctt gacttgtttc acacttatgt
      541 gcgccaaggc aaggagccac ccgagagccg ccattcattc tgcattccgg cacagtggat
      601 cagcacgctt gacccacgca tcaaagaccg catccgcact gagacccgcg ccgacatggc
      661 cgccaaggaa gccgatgacc gcgccaaaca actggaaaag tggcaggccc aggtggctgc
      721 caaaaaactt accaaagcag aatgcaccga gcggctgcgc cagcacgccg aactctggtt
      781 tggccggact gaaaacgaca ttgcccgcaa gattgaacgg gcgcatctgt attcgttcaa
      841 cctgcccaac taccgcagca gcctgagcct ggcgcaaatt gaggcttggg agagctttgc
      901 ggccacccgt gtgcgcgaag gcaaagctgc caccacgctt gaagccaaat accgcgctgt
      961 ggctgcggct ggaactgatg aactggatga aatgttgtcc tcactcgatc ccaggcatac
     1021 gatggaactc gactgggcgc ggcagtttct ggccggtgtg ccgaacgagg aatttgggcg
     1081 gcatccgcat ttgaatactt tgcaagaggt gattgcttcg caggcaaagg gggcgcgggt
     1141 tcaattgaag gaacttatca agccacgcga ggagaagatt aagaaaagtg cctatgacgg
     1201 tgtgcttccc attgtcgaaa agatcagttt ctctgacgga cggctgcatt ttcgggaaga
     1261 ccgcgaaacc gggatggatt tgtacaaggc ttgcaaaggt gatcttgtca cgtcaaaaat
     1321 caatttgcat cagggtgctg tggcgcttgc cccgtcagat ttagtcacct cgacccatta
     1381 cctgccctat gaaatcaacg tgggcgaggt gaatccggaa tatctggtgg tcatgatccg
     1441 ctcagcccag tttcgttcgt tgatagacgc gcagaaaaac agtgggatta aaaacgagca
     1501 aggtgctgag tttcttgggg agttcgagat accaattccg gatcgcaaag tgcaagagga
     1561 attagttgct gaacttaagc gatgcaacaa aattactgat tcctcattga cgatcgttga
     1621 aaattgggaa gcctacatac cgcctaccga cgacagacgg gttatcggtg agtttgtcat
     1681 tgaatcgctg tacggtattg ccgataagct caccgatcac ggagactatc cagtattacg
     1741 catgaataat ctggatgaac gaggcttttg gcatctcgat gatctcaagt ttattgaccg
     1801 taaaccatca gaagagaggc ttttgaaaca cggcgatttc atcttcaacc gaaccaatag
     1861 catagcgcta gtcggtaaat cagcagtggt tgattttgat tttccaggga cttgggctgg
     1921 ctaccttgtc cggcttcgat ttagtgatgg tctgaaccca tcatatttgc gtgacgtgct
     1981 ctcaacgcga ccgtttcgtc agatttttgc agcaattgca agaccagcag gtggccaagc
     2041 aaatctcaac gtcgaagagt tctcagatat ttctattccc tatttttcgc cagaaaagca
     2101 ggttgaaatt gttgctgcaa atcagcgcga ccgattggct atccaacaac ttgaccgcat
     2161 tggtcaagtt gcaagacgaa cctcttttca gttgctgaaa aaacattggg agtcctaacc
     2221 atgcccaagc ccatcaaaac cggtgtcttt gataacgaag cctcgacggt tgacctcaag
     2281 gtgtatgaca cgctcaaggc gcttgattgg aaacagggcg acaccttgct gtaccagcca
     2341 agttatgccc tgaccgagga cgagcaaaag gattaccccg gcagcaagtc aatcaaaccg
     2401 gattttgtat tgcaagattt gcagggcttt gcgctggcag tgatcgagaa caagctggac
     2461 gatccgcaca aggcgctgcc caagttgcga ctcaaataca gccgcttgct gaaaccccgt
     2521 tttctctatg cctgtgcctc tgatggcgcg ggcggcttga aaattctctt tttcgatctg
     2581 gcctggcgcg gtgtggatgc ggcagatttt cgggtagtgg atagcttttt gcccctggcc
     2641 gacatgaagc tgaaaattga acaagaccgg ctcaagcggc aggaactcga catcaagatt
     2701 gacaccacgc tggccggtgg ctttgacccg gcagcgggca aggaacgggc ttaccagttg
     2761 acctgcatcc tgacgctgct gcgcctgtac cgcgaaggca agatgaagat gctggttcac
     2821 atggccaccg gcctgggcaa aacgcgcacc atggtggcac tagtcaaggc gctgctggag
     2881 cacgcgctgg ccaagcgggt tttgtttgtg gtggaccggc gcacactggc gcaccaggcc
     2941 atcaacaagg gctttcgact gctggcaccg agctacaaca gccactggat tacctcgtca
     3001 agctggcgcg cccacaaaaa caagaatatc catgtggtgg tgatcgacac cctggagcag
     3061 ctttacgaca aaatcccgag caacttttac gacctgatca tcgtcgatga atgccaccgc
     3121 tcgatcacgg tcaatcgcaa tctggtgttt gaccattttg tttgcccacg catcggcttg
     3181 accgccacgc cgcgcattgc cgtcgccgcc aaagggggtg aactcaatga agatgatgca
     3241 gcgatcaacg acacctaccg tttgtttggc tgcgaggtgg gcgatccgga ttacaaattt
     3301 gacctggacc gggccattga cgagggcttt ttggcccctt accgcaagga agaacacatc
     3361 accgccctga ccaaacaggc gatggaaacc ggtgtgctct acgaccattt gctggacccc
     3421 gaaacccgca aacgcatcga gttgggtgcg cagcaaacca ttgagctgga aaagctgaac
     3481 aaacgcatcc tgagtgacga gcaagccaat cgttgggcgg aaatcattcg caaggaaacc
     3541 gagtacggtg aaaaagtcct gctgtttgca gccagtcagg cgcatagcct gatgctggtg
     3601 aaggccatca acaaggtgtt caatgattct ggccaaagcc cccgctacgc cgaggcgatc
     3661 atttcgcaaa acgatgacat caacgagtcg ctgaaggaat ggtttgagcg cccgtactcg
     3721 aacccgcgtg tggtggtgtc ggtggacatc atgagcactg gtgtcgatat tccttgcctg
     3781 cgctatatcg cctttggtgc catcacccag tcggttggca agtatttgca aatgctcggg
     3841 cgcggcaccc ggcttgaccc aaaaacaggg aagttcagtt tcaagattct ggattttgtt
     3901 ggcctgtgca agcgcatgga tgacaacggc aagggcacct tgatgcccaa tgaaaaggtc
     3961 gtcaaaccag acgctggagg tggcggcggt ggtggcggcg gcgggggtgc tgttgacggc
     4021 attctggata accctgaccc ggcaagcctg attcaacggg tgttgatgac tgtggacggc
     4081 atcaagatca ttgacaacat ccccattgcc
//
LOCUS       DAOSLL010000449         4103 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19211_length_4103_cov_4.071887, whole genome shotgun sequence.
ACCESSION   DAOSLL010000449 DAOSLL010000000
VERSION     DAOSLL010000449.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19211_length_4103_cov_4.071887"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2856
                     /locus_tag="PLB25_16785"
     CDS             <1..2856
                     /locus_tag="PLB25_16785"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOE43264.1"
                     /translation="KVNYSPYQPITTIEEAIKPGARRIHTHAENNRINHHHTAKGDIE
                     AGFAASTHILEREYEVGFQEHAYIEPETITAYIDPTERTLVITGSVQNPHRVRGFVAG
                     FLGISQSKINVKRAVMGGSFGGKDDVIDHLSCRAGLMATLTGRPVKFTYTREQSIRES
                     CKRHPYKMKYKVGLNDSGRILAIKVDILADGGGYAASTPFVTWRSVIQAAGPYNIANV
                     RVDTTGIYTNNSYTSAMRGFGSPQIVYANESLMDEIARHFNMSPVAIRQLNMLRQDDQ
                     SITGQAFTQHVVSTGEVLNLAVTKAKFEEKRDQCTKLNAEGGTIKYGIGLALSYRGCS
                     MGAEGVDTGTALVQVNHDGSISVSTSVAENGQGLQTTMSLIAAESFGVTLKNVVFSEP
                     PTSVIADGGPTVASRATLMGGRAVVDAAEKIKARIVGVIGDSIKVKSLAETEWRDGQI
                     FNKAQPEHCVSFVAAVNTSKWASVNLADYGWFAAPDIHWDEENGNGSPYFTWVYGCNI
                     AEVAIDTSTGKITMQRVTAVHDVGKVINPVGFEGQVCGGIAQGCGYGILEDFNIEHGK
                     VKSENFDSYLLPTIKDIPPIDVHAVENHDPQGPFGAKSIGEPATELAAAAINNAVNFA
                     LGTAFNKLPLTLEQVFLGYNLKKPVRQSELLISSAAKKQVLRLNSMSIRSPASLDEAL
                     DMLAEANVTALAGGTDVVVQGRMKTNAQSLINIAKLPGLCHIEERDGSLVIGGGVWFN
                     KLTEHHLVQERYPLLAQACHMVGSNQIRNRATIGGNIVNAAPCADSVPPLIAYGADLV
                     LQSAAGKRRMPVADFITNGYETRLEKGELLVEVVLPIPPANLLQNYDQLGRRNALNIT
                     RLSLTSLFAFDGQGNISLCRLVDGALLSKPQRLTAVEQVLLGKALNEASVAQALKVLE
                     KLIEDAIGGRWSAVYKQPVYLNIFRDQMNKVRAQIGQ"
     gene            2872..3330
                     /locus_tag="PLB25_16790"
     CDS             2872..3330
                     /locus_tag="PLB25_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019578113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HOE43265.1"
                     /translation="MSKISLQVNGQTVSADVEGNTRLLDFVRDSLDLTGTKEGCAVGE
                     CGACTVILNGDAVCSCLVLAAQCDNATLTTVEGLASDSLGQKLQTAFVEHGGVQCGFC
                     TPGVLMSAKALLDKNPKPSDQELLDALEGNICRCTGYQPVLQSLRAVIDQ"
     gene            complement(3506..3805)
                     /locus_tag="PLB25_16795"
     CDS             complement(3506..3805)
                     /locus_tag="PLB25_16795"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HOE43266.1"
                     /translation="MRLSKKQDRFMITLFAHPATTPLQGAENLPTVIELNVDGITCCG
                     CVVGVKKALNVLYGVREVEVKMSAGQVVVKGDGENMTQEKLASALRVAGFSVCQN"
BASE COUNT          982 a         1128 c         1114 g          879 t
ORIGIN      
        1 aaggtcaatt actcgcccta ccagccaatc accaccattg aagaagccat caagcctggc
       61 gctcgccgca ttcacacaca tgccgagaac aaccgcatca accaccatca caccgccaag
      121 ggtgatattg aggcagggtt tgccgcttcg acacatattc tggaacgtga atacgaagtc
      181 ggtttccagg agcacgccta tattgagcct gaaaccatca ccgcctacat cgacccaacg
      241 gaaagaacgc tggtgattac cggttcggtg caaaacccgc accgtgtgcg cggcttcgtc
      301 gctggctttc tgggcatttc ccagtcaaaa atcaacgtca agcgggcggt gatgggcgga
      361 tcattcggtg gcaaggacga tgtgattgac cacctgtcct gccgcgccgg attgatggcc
      421 accttgaccg ggcgaccggt caaattcacc tacacacgcg aacaatccat ccgcgaaagc
      481 tgcaagcgtc atccctacaa gatgaagtac aaggtagggc tcaatgacag tggccgcatt
      541 ctggccatca aggtcgatat cctggcagac ggcggcggct atgcggcatc cacgccgttt
      601 gtcacctggc gctccgtgat ccaggcggcc ggcccctaca acattgccaa tgtgcgtgtg
      661 gatacaaccg gcatttacac caacaacagc tacacctcgg cgatgcgcgg ttttggttcg
      721 ccgcaaatcg tttatgccaa tgagtctttg atggatgaaa ttgcccgaca tttcaacatg
      781 tcgcccgttg caatccgcca gctcaacatg ctcaggcaag acgaccaaag catcaccggt
      841 caggccttca cccaacatgt cgtctccact ggcgaagtgc tgaacctcgc cgtcacgaag
      901 gcgaaatttg aagagaaacg cgaccagtgc acaaagctca acgccgaagg tgggactatc
      961 aaatacggta tcggcctggc actaagttac cgtggctgct cgatgggtgc ggaaggggtt
     1021 gacacaggta ccgcgctggt tcaggtgaac catgatggca gcatcagcgt gtcaacttcg
     1081 gtggctgaaa acgggcaagg gctgcaaacc acgatgtccc tgatcgctgc tgaatcgttt
     1141 ggtgtcaccc tgaaaaacgt ggttttcagc gagcccccga catcggtcat tgccgacggt
     1201 ggcccaaccg ttgcatcccg tgcaaccctg atgggcgggc gggccgtggt ggatgccgcc
     1261 gagaagatca aggcgcgtat tgtgggtgtg atcggggaca gcatcaaagt caagagtctg
     1321 gcggaaaccg aatggcgaga cggccagatt ttcaacaagg cgcagccaga gcactgcgtt
     1381 tctttcgtcg cagccgtcaa taccagcaaa tgggcatcgg tcaatttggc agactacggt
     1441 tggtttgccg cgcccgatat ccattgggac gaagaaaatg gcaatggcag cccttacttc
     1501 acctgggttt acggctgcaa tatcgccgaa gtcgccatcg acaccagcac cggcaaaatc
     1561 accatgcagc gcgtcactgc cgtgcacgac gttggcaagg tcatcaaccc ggtgggtttc
     1621 gaaggccagg tgtgcggtgg cattgcacaa ggctgcggct acggcattct ggaagacttc
     1681 aacattgagc acggcaaagt caaatcagag aactttgatt cttacctgtt gccaaccatc
     1741 aaggacattc cgccgattga tgttcatgcg gttgaaaacc atgacccaca aggtccgttt
     1801 ggtgccaaga gcattggtga gccggccacc gaattggcgg cggcggccat caacaatgcg
     1861 gtcaattttg cgttgggtac tgcattcaac aagctgccgc tcacgctgga acaggtgttt
     1921 ttgggctaca acctgaaaaa accggtacgc caaagcgagc tgttgatctc gtccgctgcg
     1981 aaaaaacaag tgctgcgttt gaacagcatg agcattcgca gtccagcctc gcttgacgaa
     2041 gcactggaca tgttggcaga agctaacgtg accgcgctgg ctggcggtac cgacgtggtg
     2101 gttcagggac gcatgaagac caatgcacaa agcctgatca acattgccaa gcttcctggc
     2161 ctatgccata tcgaagaacg tgatggttcg ctggtgattg gtggcggtgt ctggtttaac
     2221 aaactgaccg agcaccattt ggtacaagag cgttatccgc tgctggctca agcatgccac
     2281 atggtggggt ccaaccagat tcgcaaccgt gcaacgattg gcggaaacat cgttaacgca
     2341 gcaccttgcg cggactcggt tccgccactg attgcgtatg gcgcagacct tgtgctgcaa
     2401 tcggcggcag gcaagcgccg catgccggtt gccgatttca tcaccaatgg ctacgaaacc
     2461 agactcgaaa aaggggagtt gctggttgag gttgttttgc ccataccacc tgccaatttg
     2521 cttcaaaact acgatcaact cggtcgccgt aacgcgctca acatcacacg cttgagcctg
     2581 accagcctgt ttgcatttga tggccagggc aacatcagcc tgtgccgcct ggttgacggt
     2641 gcactactga gcaaacctca acgcctcact gcggttgaac aggttttgct gggcaaagcg
     2701 ctcaatgagg ccagtgttgc acaggcgctg aaagtgctgg agaaattgat tgaagatgcg
     2761 attggcgggc gttggtctgc cgtctacaaa cagcctgttt acctcaatat tttccgcgac
     2821 caaatgaaca aggtccgagc ccaaatcggc caataacccc taggaaaagt gatgagcaaa
     2881 atttctttac aagtgaatgg gcaaaccgtc agcgcagatg ttgaaggaaa tacccgcctg
     2941 ctcgactttg tgcgggattc actcgacctg accggtacca aagaagggtg tgcggtgggt
     3001 gagtgtggtg cttgcactgt gatcttgaat ggcgatgcgg tctgctcctg cctggtattg
     3061 gctgcgcagt gcgataacgc cacgctgaca acggtcgaag gattggcatc agacagcctg
     3121 ggtcaaaagc tgcaaacagc gttcgtcgaa catggcgggg ttcaatgtgg cttctgcacc
     3181 cctggcgtat tgatgagcgc caaggcatta ctggataaaa acccaaagcc cagcgaccag
     3241 gaattgcttg acgcattgga aggcaacatt tgccgctgca ctggttatca acctgttttg
     3301 caatcattgc gcgctgtgat cgaccaatag gcactggcca aaatacaagc tgtgacgaga
     3361 ccgtgccttg gttgttttac atttaaatta atagcacgta gcgcttattc catgcgctct
     3421 acagccgtat ttcatccata aatctgaaga aaacctgaag tcactggggc atcggcctgg
     3481 cccggtgggc tgtttcgggt aaacatcagt tttggcaaac cgagaaaccg gcaacgcgca
     3541 gcgcgctggc aagtttttcc tgcgtcatgt tctcgccgtc acctttgaca accacctgac
     3601 ctgccgacat tttgacctca acctcacgca cgccatacaa cacattcagc gcttttttga
     3661 cacctacgac acagccgcag caagtgatgc catccacatt caattcaatc actgtgggta
     3721 aattttccgc tccttgaagt ggtgttgttg ctggatgagc gaagagtgtg atcatgaacc
     3781 tgtcctgttt tttcgaaagc cgcaagccac catcctagcg ggcttcaagc agcaccacaa
     3841 gttatcaaac tgatctatcg gtattgattt gatcctggct gatgccattc gccgcacccg
     3901 agtcggcggc aagcggcgcg tctgctgccc accagcgcac agcacgccac agaagttggt
     3961 gaccagtatg gacgtggcgg gaagttattt ttgaccatgc cctgaggcgg acgagccgca
     4021 ataggacggc cttcgtcgcg agggcgcgac tcctgcacaa acagtcggcg cctatttgaa
     4081 tttgtaggag tcgcgccctc gcg
//
LOCUS       DAOSLL010000450         4100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19229_length_4100_cov_2.989864, whole genome shotgun sequence.
ACCESSION   DAOSLL010000450 DAOSLL010000000
VERSION     DAOSLL010000450.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19229_length_4100_cov_2.989864"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..695
                     /gene="ddpX"
                     /locus_tag="PLB25_16800"
     CDS             123..695
                     /gene="ddpX"
                     /locus_tag="PLB25_16800"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HOE43267.1"
                     /translation="MPLKEITHPAIDIDLRYATSDNITGSPIYNHAMAFLHKDALVLL
                     VRAAELAAAQGFRLRVLDAYRPAAAQWRLWRALPNPKFVADPRIGSMHTRAVAVDLTL
                     CAANGVPLDMGTEFDEMTPQSGHARCDIAPQAQRNRCLLLGIMTGAGWDHHPHEWWHY
                     NLPEPTRYPKLSDKVEGEFLMDSREPQRAV"
     gene            complement(692..1516)
                     /locus_tag="PLB25_16805"
     CDS             complement(692..1516)
                     /locus_tag="PLB25_16805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HOE43268.1"
                     /translation="MIHTQKMPVPAKPGKTPTQSTPAAPAGERLSKRVMQLKACSRRE
                     AEQYIEGGWVQINGQVVEEPMARVRDQTVTLDPHANLMQINDVTLLLHKPPAFDAMAA
                     LGQANKRVKPAQQLLQPASHWVEDPSGMRILKHHFAKLSACVPLELAASGLVVFTQDW
                     RVLRKLTEDATIMEHELLAEVKGEVTAELLRRLNAPPASDRDAVPVVKVSVNSSSETS
                     TRLRFAFKGAHPGLIAWLCERAGLQITSMKRLRIGRVALSHLPEGQWRYLHPQERF"
     gene            2454..>2743
                     /locus_tag="PLB25_16810"
     CDS             2454..>2743
                     /locus_tag="PLB25_16810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE43269.1"
                     /translation="MAKLRQIAFYGKGGIGKSTTSQNTLAALSDLEQKILIVGCDPKA
                     DSTRLILHAKAQDTILSLAAEAGSVEDLELEDVMKIGYKGIRCVESGGPEPGV"
     assembly_gap    2744..2753
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2754..3325
                     /gene="nifH"
                     /locus_tag="PLB25_16815"
     CDS             <2754..3325
                     /gene="nifH"
                     /locus_tag="PLB25_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013234820.1"
                     /note="nitrogenase iron protein; nitrogenase component 2;
                     with component 1, an molybdenum-iron protein, catalyzes
                     the fixation of nitrogen to ammonia; nitrogen reductase
                     provides electrons to the nitrogenase complex; in R. etli
                     there are three essentially identical copies of nifH which
                     are actively expressed during symbiosis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrogenase reductase"
                     /protein_id="HOE43270.1"
                     /translation="TSINFLEENGAYDNMDYVSYDVLGDVVCGGFAMPIRENKAQEIY
                     IVMSGEMMAMYAANNISKGVLKYANSGGVRLGGLVCNERQTDKEYELADALAGMLGTK
                     LIHFVPRDNIVQHAELRRMTVIEYAPESKQAAEYRALASKIHNNGGNGTIPTPITMDQ
                     LEDMLMEFGIMDTIDETIVGKKADELVAA"
     gene            3487..>4100
                     /locus_tag="PLB25_16820"
     CDS             3487..>4100
                     /locus_tag="PLB25_16820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOE43271.1"
                     /translation="MTATVEERKATNKALIDEVLKAYPEKMAKRRAKHLGSFESGKPD
                     CGVKSNIKSLPGVMTIRGCAYAGSKGVVWGPIKDMVHISHGPVGCGQYSWANRRNYYI
                     GTTGIDSFVTMQFTSDFQEKDIVFGGDKKLDKIIDEIEVLFPLNKGISIQSECPIGLI
                     GDDIEAISKKKSKQYAGKTIVPVRCEGFRGVSQSLGHHLANDAIR"
BASE COUNT          888 a         1206 c         1162 g          834 t
ORIGIN      
        1 tgcggtatcg gtggagcgtc gaattaccca cccctgcgtg ctggccatca ccgatgcggc
       61 ccgcgggcag ttgttcagtg tctgactttc aaggatggtg cttgcccgga taatcgcgcc
      121 ccatgccgct gaaagaaatt actcaccctg ccatcgacat cgacctgcgc tacgccactt
      181 ccgacaacat caccggcagc cccatttaca accacgccat ggcctttttg cacaaggatg
      241 cactggtttt gctggtgcgc gcggctgaat tggcagcggc ccaggggttc agattgcgcg
      301 tactcgacgc ttatcgtccc gccgccgccc aatggcggct gtggcgggcc ttgccgaacc
      361 cgaaatttgt cgctgaccca cgcattggct ccatgcacac ccgcgccgtg gcagtggacc
      421 tgacactgtg cgcggccaat ggcgtgccgc tggacatggg cactgagttt gatgaaatga
      481 caccacagtc cggccatgcc cgctgcgaca ttgcaccaca ggcccagcgc aaccgctgtt
      541 tattgctggg catcatgacc ggggccggct gggaccacca tccgcatgaa tggtggcatt
      601 acaacctgcc cgagccaacc cgctacccca agctcagcga caaagttgaa ggtgaatttt
      661 tgatggacag ccgcgagccg caaagggctg tctaaaaccg ctcctgcgga tgcaggtagc
      721 gccactgacc ttcgggcaaa tgtgacaatg caacccgacc gatgcgcagg cgcttcatgc
      781 tggtgatttg cagcccagcc cgttcgcaca accaggcaat caggcctggg tgcgcgccct
      841 tgaaggcaaa gcgcagccgg gtcgatgtct cgctgctgct gttgacgctg accttgacaa
      901 ccgggacggc gtcacggtca ctggctggcg gggcgttgag tcggcgcagc aattcggcgg
      961 tgacttcgcc cttgacttct gccagcagtt catgctccat gatggttgcg tcttcggtca
     1021 atttacgcag cacgcgccag tcctgcgtaa acaccaccag cccgctagct gccaactcaa
     1081 gcggcacgca ggcgctgagc ttggcaaagt ggtgcttcaa aatgcgcatg cccgatgggt
     1141 cctcgaccca gtggctggca ggctgcagca actgttgcgc cggtttgaca cgcttgttgg
     1201 cctggcccag tgccgccatg gcatcgaatg ctggcggctt atgcagcagc agcgtgacat
     1261 cattgatctg catcaggttg gcgtgcgggt caagggtcac ggtctggtcc cgcacccgcg
     1321 ccattggctc ttccaccact tgtccgttga tctgcaccca gccaccttca atgtactgct
     1381 cggcctcgcg gcgggaacag gctttgagct gcatcacgcg cttggaaaga cgctcacccg
     1441 ctggtgctgc gggcgtggac tgtgtcgggg ttttgcctgg tttggccgga acgggcattt
     1501 tttgagtatg aatcatggca gccgatggta actgccaccc tgtttgcagc gacgggcagg
     1561 tgcatggtgc aatgatctgg ttcttcgccg ttcagtgtgc aagctgcatt ggtagatcag
     1621 catggcaatc gggcaactgc gaagcaccca cttgactgtc tggctgcgcc agcccaaccc
     1681 gaactctgcc gggatggcac caaggcttgc ctacgcggcc tggcaaatgt gtcggcttgt
     1741 ctgctgctca ggtgcagatg atttgtgtca ccctgcgcgc cgatgttgtg caagcgctcg
     1801 agaagacttt cgagctcacc accttccctg cccatggcca ttttcaagcc gtgcggatgg
     1861 ccatgcagcg cctgggaatt ggcattttgc ctggcctcca agcgttgtcg tgcgcgcgac
     1921 ctggtggctt gcgtggtggc cgcacgcatc gtttgtccgg ccaccaacct ggcaacaacg
     1981 tgcctgagac ccaccagcaa ttccattccc aaatcacccg aaattcctcc gttggacgcg
     2041 cccgcgtggg ccgctggcgg cgtggctggg tacagggcat ccgccagtgc ccaatcgctt
     2101 catccaactg gctgtctgcc ctgacaggct gcaaacgcga cacaagctgg cgagatggtc
     2161 ggccattcag gggcttttac gacacgcgct caggttttgt cgggaaatca acaaacccgc
     2221 aaaaatgacc gaaaacattc cctaacttgt tgatttaatt gaaaacaagc aatcatttgg
     2281 cagcacttca agaggccccc ttttacatgg cacggaagct gcaataaaca agaagcgggg
     2341 cacaagtccg gccgacgtga acctaaaaaa ataagaccca caggcacgaa agctgaccta
     2401 agtcgcggca acgcaagctt ggttcttaac tttctcttca ttggagtaat gacatggcaa
     2461 aacttcggca aatcgccttc tatggcaaag gtggcatcgg caaatcgacc acttctcaaa
     2521 acaccctggc agcattgtct gacctggagc aaaaaatcct gatcgtcgga tgcgacccca
     2581 aagccgactc gacccgactg atcctgcacg ccaaggcaca agacaccatc ctgtcgctcg
     2641 ccgcggaagc cggctcggtg gaggatctgg agcttgagga cgtgatgaag attggttaca
     2701 agggcatccg ctgcgtcgag tccggtggcc cggagccagg agtnnnnnnn nnnttacctc
     2761 tatcaacttt ctggaagaga acggcgctta cgacaacatg gactatgtat cgtatgacgt
     2821 actcggtgac gtggtgtgcg gtggttttgc catgccgatc cgcgaaaaca aggcgcagga
     2881 aatctacatc gtcatgtctg gcgaaatgat ggccatgtac gctgccaaca acatctccaa
     2941 gggtgtcctg aaatacgcca actcgggtgg tgtgcggctc ggcgggctgg tctgcaatga
     3001 gcgccagacc gacaaggaat atgaactggc cgacgcactg gctggcatgc tgggcaccaa
     3061 gctgatccac ttcgtgccgc gcgacaacat cgtgcaacac gctgaactgc gccgcatgac
     3121 ggtaattgag tacgctcccg aatccaagca ggcggctgaa taccgtgcct tggccagcaa
     3181 gattcacaac aacggtggca acggcaccat tccgaccccg atcaccatgg accaactcga
     3241 agacatgctg atggagttcg gcatcatgga caccatcgac gaaacgattg tcggcaagaa
     3301 ggctgacgag ctggttgctg cttgacgaga aggcaacgac ccgcaccccg tttttgccga
     3361 cttacccgca tttttgtggg gccgactgag ctcggcctgg tcgattgaca tcggccccgc
     3421 aaaaaaggcc acgcggtacc ttggcaagtt caatcccacc aagacaattt actaccggag
     3481 ttccccatga ctgccaccgt tgaagagcgc aaggccacaa acaaggccct gattgatgaa
     3541 gtgctgaaag cctatcctga aaagatggcc aagcggcgcg ccaaacacct gggctcgttt
     3601 gagagcggca agcccgactg tggtgtcaaa tccaatatca agtcattgcc tggcgtgatg
     3661 acgattcgag gctgcgccta tgccggctcc aaaggcgtgg tgtggggccc aatcaaggac
     3721 atggtccaca tcagccacgg cccggtgggt tgcggccagt attcatgggc caatcgacgc
     3781 aactattaca ttggcacgac cggcattgac agctttgtga ccatgcagtt cacctcggac
     3841 tttcaggaaa aggacattgt ttttggcggc gacaagaagc tcgacaaaat catcgacgaa
     3901 attgaggttt tgtttccgct gaacaagggt atttcgatcc agtccgagtg cccgattggc
     3961 ctgattggtg acgatatcga agccatctcg aagaagaaaa gcaagcaata cgcaggcaag
     4021 accattgtgc cggtgcgctg cgaaggtttt cggggtgtca gccagtcgct cggtcaccac
     4081 ctggccaatg atgcgattcg
//
LOCUS       DAOSLL010000451         4093 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19260_length_4093_cov_5.951213, whole genome shotgun sequence.
ACCESSION   DAOSLL010000451 DAOSLL010000000
VERSION     DAOSLL010000451.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4093
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19260_length_4093_cov_5.951213"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            319..1452
                     /locus_tag="PLB25_16825"
     CDS             319..1452
                     /locus_tag="PLB25_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HOE43272.1"
                     /translation="MQQFKFVGAALSAALLVAACGGGSDDDQAAVVKFSSMVSFGDSL
                     SDVGTYKVGTVAALGGGLYTVNGIAGAIGAVPTPSKNWTELIAAQAGLPAPCPAQTGL
                     DGAPAQGFSVPVANHLGCYGYAQGGSRVTNPVGPGNKLLGGVNATLGQLTVPVVTQIA
                     QHLAAAGGSFKGNELVTVMAGGNDVFINLATITPAITSLVTGGMSVAAATAQASTAAV
                     TEMGKAGAELAAYTKAEIVGKGAKYVVMVNLPDVSKTPFAYSQDAVTQGLINTMVVTF
                     NTQLQNGLAGTPGIVIVDAYSDSRNQAANPTAYGLSNVTVPACDLSATANPLASSLVC
                     TSKNVVPGDVSQYLFSDTVHLTPYGYKLVAQLVAKNLVQVGWL"
     gene            1449..1691
                     /locus_tag="PLB25_16830"
     CDS             1449..1691
                     /locus_tag="PLB25_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43273.1"
                     /translation="MNRRSGQAQQAQLAVVAAAGLTLGNWVLARLAVASMLLGELRAI
                     ADPPESPASPWPGHYDAAWRTDHHCLPVFAVGFVGA"
     gene            complement(1721..1900)
                     /locus_tag="PLB25_16835"
     CDS             complement(1721..1900)
                     /locus_tag="PLB25_16835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43274.1"
                     /translation="MSIYFFSFDVAQPLGDNATHPIEPLATRLGALLLLAGRSPQRRR
                     AYPDAPPAAHSGASN"
     gene            complement(2151..3248)
                     /locus_tag="PLB25_16840"
     CDS             complement(2151..3248)
                     /locus_tag="PLB25_16840"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013723208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOE43275.1"
                     /translation="MIRSGTDPDSIAASADCIRAGGLLGLPTETVYGLAADADNDTAV
                     AKIFAVKGRPSDHPLIVHVANAQAVDHFATDIPDFAKVLMSAFWPGPLTLILPRRPGI
                     AAAAAGGHPTIGLRCPSHPVAQALLNWGQSRINRDKSEPGEKECDSDPNYFWGLAAPS
                     ANQFGRVSPTTAAHVQGEFGDDLLILDGGPCSVGIESSIIDCTRGRPVLLRPGAITPS
                     QVQAVCGFEVLQKHELPEHAARAPQASGTLESHYAPQARVRLMEASELQSELARQDAI
                     FGTDCLVASPSPGGLAPLLAVWSRSRLGAATSPQVWHQAMPDDAASAARQLFAVLRDF
                     DALGVRQIWIETPPTDTSWDGVRDRLQRAAS"
     gene            complement(3277..>4093)
                     /locus_tag="PLB25_16845"
     CDS             complement(3277..>4093)
                     /locus_tag="PLB25_16845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HOE43276.1"
                     /translation="PGILKTARLGYDGKGQVRVSSRTELAAAWDALKYLPCVLEKLLP
                     LAAECSVIVARGWDGACVNFPVQLNLHRQGILAVTQVYDENLPAALMAQAVAATKQIA
                     DELCYVGVLCVEFFVLAEGSPERASLGALVVNEMAPRPHNSGHYTLDACDVSQFELQV
                     RTLTGLPLVQPRQHSAAIMLNLLGDLWINRGQSRINSDENDLVAKEFDSDPNYSPPWA
                     KVLALPGVHLHLYGKLKASKGRKMGHLTVTAASAPEARCTALAAARLLGMAAF"
BASE COUNT          783 a         1275 c         1239 g          796 t
ORIGIN      
        1 ccgtgcatcc caactccttg gtgcaaaacg gggagttatt cccgaccaca tcctgtgggc
       61 ctgcagaccc ggcacaaact ggcagcaagt cgatgccaat cagtcgctga catgctctgc
      121 aagggccaac agtctgagcg ccgcccttcg atacagtcct gagcaaagct gatgcgagcg
      181 ccgtgccatc gctgccggtc ggcactgccg ttttgatcgg gcgcacgggt tttcccgata
      241 ttcgcgcggc gttccagcgg ctagactggt tgcagcaaaa tagtacggtc gttctttttt
      301 aatttttgga gttatcggat gcagcagttc aaatttgtcg gtgcggcgct cagcgccgcc
      361 ttgttggtgg cagcttgcgg cggcggcagt gatgatgatc aggcagcggt ggtcaagttt
      421 tcatccatgg tgtcctttgg cgacagcctg agtgatgtcg gcacttacaa ggtcggcacc
      481 gttgctgcgt tgggtggtgg tttgtacacc gtcaacggta ttgcgggtgc catcggggcg
      541 gtgccgacac cttccaaaaa ctggaccgaa ctgatcgccg cacaggccgg tttgccagcc
      601 ccgtgcccgg cgcaaaccgg gcttgatggt gcccccgccc aaggctttag cgtgccggtg
      661 gcaaaccacc ttggctgcta tggctatgcg cagggtggct cccgcgtgac caacccggtc
      721 ggtccgggca acaagttgct cggtggtgtc aatgccacgc tcgggcaact gactgtgccg
      781 gtggtgacgc aaatcgccca gcacctggcg gcggcgggcg gcagcttcaa gggtaacgaa
      841 ctggtcacgg tgatggccgg gggcaatgat gtcttcatca acctggcaac gatcacgcca
      901 gccatcacca gcctggtcac cggtggcatg agtgtggcgg ccgccaccgc ccaggcatcg
      961 actgctgcag tcaccgaaat gggcaaggcc ggggccgaac tggcggccta caccaaggcg
     1021 gaaattgttg gaaaaggtgc caaatacgtg gtcatggtca atctgcccga tgtcagcaaa
     1081 acgccttttg cctactcgca ggatgccgtc acgcaaggct tgatcaacac catggtggtc
     1141 accttcaaca cgcagttgca aaacggtctg gcgggcacgc ccggcatcgt catcgtcgat
     1201 gcctacagtg acagccgcaa ccaggccgcc aacccgaccg cctacggctt gagcaacgtc
     1261 accgtgccag cctgtgatct gagtgccacc gccaatccac tggccagttc gctggtatgc
     1321 accagcaaga acgtggtgcc tggtgacgtt tcgcagtacc tgttttccga caccgtgcac
     1381 ctgaccccct acggctacaa gctggtggcc cagctcgtcg ccaaaaacct ggtccaggtt
     1441 ggctggcttt gaatcgccga agcggccagg cgcagcaagc ccaactggcg gtcgttgccg
     1501 ctgccgggct gacgttgggt aactgggtat tggcccgcct tgctgttgct tcgatgctgc
     1561 ttggcgaact gcgcgccatt gctgatccgc ccgaatcacc agccagcccc tggccaggtc
     1621 actacgatgc tgcttggcga actgaccacc attgcctgcc ggtgtttgca gtgggttttg
     1681 tcggtgcctg agggccggtc ggacttttaa cctagattcc tcagttggac gcgcccgagt
     1741 gggccgctgg cggcgcgtct gggtaggcgc gacgacgctg cgggctcctg cccgctagga
     1801 ggagcaacgc ccccaggcgc gttgccagcg gctcaatcgg gtgcgtagcg ttgtcaccca
     1861 acggttgagc cacatcgaag ctaaaaaagt aaatgctcat aaggacttat agaggaaaac
     1921 aagcggtcaa ctgaggaatc taggtttaag caattgattc atggggtggg cgcagccatg
     1981 ccaccaggct ggcctgcttc aagccgtcca aacgcgcgca ggcacgtttt gagccctgca
     2041 atttcgccct agccaaaccg gctgccagcg gcctgcctga gcgcgtccaa ccggggaatc
     2101 gaaactgaag tcgttgccgc gcaactgcgc tggttgatca gcggctgcgg tcagcttgcg
     2161 gcccgctgca agcggtcacg cacgccgtcc cagcttgtgt cggtcggcgg cgtttcgatc
     2221 catatctgcc tgacacccag ggcatcaaaa tctcgcagca ccgcaaacag ttgccgcgcg
     2281 gcgctggccg catcgtcggg catcgcttga tgccaaacct gcgggctggt tgctgcgccg
     2341 agccgactgc gtgaccagac cgccaacaga ggggccaaac cgcccggcga cgggctggcg
     2401 accaggcagt cagtgccaaa aatggcatct tgcctggcca gttccgactg caattcagat
     2461 gcctccatca gccgcaccct ggcttgcggg gcgtaatgcg attccagtgt cccggaggcc
     2521 tgtggtgccc gtgcagcatg ctctggcagc tcatgttttt gaagcacttc aaaaccgcac
     2581 acagcctgca cctggctcgg cgtgatggcc cctggccgca acaagaccgg tcggccacgg
     2641 gtgcagtcga tgatgctcga ctcaattccc acgctgcacg gcccgccgtc caaaatcagc
     2701 aaatcatcgc caaactcgcc ctgcacatgc gcagccgtgg tcgggctgac gcgcccgaac
     2761 tgattcgcac tcggtgccgc aagcccccaa aaataattgg ggtcagagtc gcattctttt
     2821 tcgccaggtt ctgatttgtc acgattaatt cgactctgac cccaattcag cagcgcctgg
     2881 gcgaccgggt gcgacgggca gcgcaagccg atggtcgggt gaccaccggc ggcggcggcg
     2941 gcgatgccgg ggcggcgtgg caggatcagg gttagtgggc cgggccaaaa ggcactcatc
     3001 agcaccttgg caaaatcggg tatgtcggtg gcgaagtggt ccaccgcctg cgcattggcc
     3061 acgtgcacga tcagcgggtg gtcgcttggg cggcctttaa ctgcgaagat ttttgccacc
     3121 gccgtgtcat tgtcggcatc ggccgccagc ccgtaaaccg tttcagttgg caagcctaat
     3181 agccctccag cccttatgca gtctgcgctg gcagctattg aatcaggatc agtgccggac
     3241 cggatcacag cgctgcccaa aacaggctgg ctgaactcaa aatgccgcca tgcccagcaa
     3301 gcgggctgcg gccagcgcgg tgcagcgggc ctctggcgcg ctggccgcag tcaccgtcag
     3361 atgccccatc ttgcgcccct tgctggcctt gagcttgccg tacaaatgca aatgcacacc
     3421 cggcaatgcc agcaccttgg cccagggagg cgaataattg gggtcagagt cgaattcttt
     3481 tgcgactaaa tcgttctcat cggaattaat tcgactctga ccccgattta tccagaggtc
     3541 gccgagcagg ttgagcatga tggcggcact gtgctggcgc ggctgcacca gcggcaggcc
     3601 ggtcagggtg cgcacctgca gttcaaactg cgacacgtcg caggcatcga gcgtgtagtg
     3661 gccgctgttg tgcgggcgcg gtgccatttc gttgaccacc agtgcaccca gcgacgcgcg
     3721 ctcgggcgaa ccttctgcca gcacaaaaaa ttcaacacac agcacaccta cataacacaa
     3781 ctcatcagct atttgttttg tagctgctac cgcttgtgcc ataagggctg caggtagatt
     3841 ttcgtcataa acctgggtga ccgccaagat tccctggcgg tgcaggttga gctgcaccgg
     3901 aaagttgacg caggcaccgt cccagccccg cgccacgatc accgagcact cggcggcgag
     3961 cggcagcaat ttttccaaca cgcagggcag gtacttcagc gcatcccagg ccgccgccag
     4021 ctcggtccgg ctgctgactc gcacttgccc tttgccgtcg taacccagcc gggcggtttt
     4081 cagaatgccg ggc
//
LOCUS       DAOSLL010000452         4086 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19299_length_4086_cov_5.255272, whole genome shotgun sequence.
ACCESSION   DAOSLL010000452 DAOSLL010000000
VERSION     DAOSLL010000452.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19299_length_4086_cov_5.255272"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..688)
                     /locus_tag="PLB25_16850"
     CDS             complement(119..688)
                     /locus_tag="PLB25_16850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HOE43277.1"
                     /translation="MNLAARHRVISVDEYLQGETRSVIRHEYLAGEVYAMAGAGEKHN
                     RIAGNLFFHLRAASRGKTCGVFISDMKLRIDASDSFYYPDVLVSCDPQDTESLFKKLP
                     CLLVEVLSPSTESIDRREKLLAYRKLPTLRHYLLVSQEQRWVQWHLRDDADQWLTSEF
                     EGGGTLELACQGLALSVSLDDIYEDVNLA"
     gene            complement(798..1418)
                     /locus_tag="PLB25_16855"
     CDS             complement(798..1418)
                     /locus_tag="PLB25_16855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HOE43278.1"
                     /translation="MPRRTKEDAQTTRLQLLEAAVRVFAEKGVSRTSLQDIAQAAGAT
                     RGAIYWHFKNKADLFNAMMEGAILPIEQAMQQIGHDPALDPLAELERAMLQAMHGIVH
                     DPRTRAIFEIATLKVEYVDELLAVKTRHAQAHANGTLQLQRSLLEAAARRGIALSVPP
                     ELAARGLQALMAGLIHTWILAPTEFELLPVAQAAIRAHLNGLGLTR"
     gene            1644..2858
                     /locus_tag="PLB25_16860"
     CDS             1644..2858
                     /locus_tag="PLB25_16860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOE43279.1"
                     /translation="MLLLSACGQGSAPPAGGPGGAMPPAEVGVVTATPGDVGLITELP
                     GRLEASRVAQVRARAAGILQKRLFVEGSDVRAGQALFQIDASPYEATLASAQASVAKA
                     EANLMQASAQAERFKPLIEAKAISQQEFINAQAAQKQAQADVAVARAAVQTARINVAY
                     ANITAPIAGRIGRALVTEGALVGQGEATQLAVIQQIDPIYVNFTQSAAEVMKLREAME
                     SGQLKRAGSQGASVRLLLEDGRLYPRAGKLLFSDLTVDSTSGQITLRAEMPNPGGTLL
                     PGLFVRVRLEQAQASNAISLPQQAVTRSPQGDSVMVVTPEGQAVARPVKIGGQQNGQW
                     VILSGLQAGEQVMVDGFQKLRGPAPVKAVPWQANSPAKAASASAGASAATSAVAATSA
                     SVPASAAVSAAR"
     gene            2871..>4086
                     /locus_tag="PLB25_16865"
     CDS             2871..>4086
                     /locus_tag="PLB25_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HOE43280.1"
                     /translation="MAKFFIERPIFAWVIALFIIVMGAVSISQLPIAQYPPVAPPSIV
                     ISATYPGASAQTLEDSVLSIVEQEMNGSPGLIYMESVAQANGTGSITLSFQPGTSPDL
                     AQVDVQNRLSRATPRLPSAVTQQGVRVEKSRSNFLLFAILSSDDPALNPVALGDYASR
                     SIVPEIQRLPGVGQVQLFGTERAMRIWIDPAKLLGFNLSAADVTAAIRAQNAQVSAGE
                     IGSLPNVAGQGISATVVVNGQLGTVEQFGNVTLRANADGSSVRLKDVARIELGAQTYA
                     TSARLNGKPSTGVGVQLSPSGNALATAKAVRARMQQLEKFFPKGMSWTIPYDSSRFIE
                     TSLRQVAETLVEAIVLVFLVMFLFMQDWRYTLIPTLVVPVALLGTFATLLGLGFSINV
                     LTMFGMVLVIGIV"
BASE COUNT          750 a         1248 c         1278 g          810 t
ORIGIN      
        1 cttgtgcctc ctgggtcaat gccaattcac cagccgcgtc gggtgtttgc tttttcagcg
       61 atgccaagcc aaacaacgcc gtgcggcacc gatcggaagt gcttggccgc agcagtggtc
      121 acgccaggtt cacatcctcg taaatatcgt caagcgacac gctcagcgcc aggccctggc
      181 aggccagttc cagcgtgcca ccgccttcaa actcgctggt cagccactgg tcggcatcgt
      241 cgcgcaggtg ccactgcacc cagcgttgtt cctgtgacac cagcaggtag tggcgcagcg
      301 tcggcagttt gcggtaggcc aacagttttt cgcggcggtc aatcgactcg gtggagggtg
      361 acaacacctc caccagcagg cagggcagct ttttgaacag cgattcggtg tcctgcgggt
      421 cacaactcac cagcacgtcg gggtaataaa agctgtcgct ggcatcaatg cgcagtttca
      481 tgtcgctgat gaacacgcca caggtcttgc cacggctggc ggcgcgcaga tggaaaaaaa
      541 ggttaccggc aatgcggttg tgcttttccc cggcaccggc catggcatac acctcgccag
      601 ccaggtattc atggcgaatg acgctgcggg tttcaccctg caggtactca tcgacggaga
      661 tgacgcgatg tctggcggca agattcatgc tgttggctcc ggtgttcggg tggcttggct
      721 ggcgctgctg gtgaaaaacc gtttgtcggt tcgcggcaaa agtctatcag gctgggccag
      781 atgtggtttg actcacatca gcgggtcaac cccagcccgt tcaggtgggc tcgaatggca
      841 gcttgggcca ccggcagcag ttcaaattcg gttggtgcca ggatccaggt gtgaatcaac
      901 ccggccatca gcgcctgcaa gccacgcgcc gccagttcag gtggcaccga cagggcgatg
      961 ccacgtcgcg cagcggcctc cagcaggctg cgttgaagtt gcagtgtgcc atttgcatga
     1021 gcctgcgcat gccgggtttt taccgccagc aactcatcga catactccac tttcagggtg
     1081 gcgatttcaa atatcgccct ggtgcgcggg tcatggacga tgccgtgcat ggcctgcagc
     1141 atggcccgtt ccagttcggc caaagggtcc agcgccgggt cgtggccgat ttgctgcatg
     1201 gcttgctcaa tcggcaggat ggccccttcc atcatggcgt tgaacagatc agccttgttc
     1261 ttgaaatgcc agtaaatcgc cccccgggtg gctccagcgg cttgtgcaat gtcttgcagc
     1321 gaggtgcgcg acaccccttt ttcagcaaac acccgcaccg ctgcttccag caattgcaag
     1381 cgggtggttt gggcatcttc tttggttcgg cggggcatct gggaagtgaa gggcgtgagg
     1441 ttcgtgattg acaaaataca ttcatcattg tatgtataat ttcctgcaaa attccaactc
     1501 tcaccgtcca cgcagggtta accctgtatt tttcggaaaa caccatgctt ctcacccagt
     1561 ccccgtcggc cacgctgccc cttgccaaag ccagactgta ccatccccgc gtgatcgcat
     1621 ccggcgctgc ggccgcgagc ctgatgttgc tgctgtccgc ttgcggccag ggtagcgcgc
     1681 cgcccgctgg tggccccggt ggcgcgatgc cgccagccga agttggcgtg gtgacggcga
     1741 caccaggcga tgtgggcctg atcaccgaac tgccgggtcg tcttgaagcc tcccgcgtcg
     1801 cccaggtgcg ggcgcgtgcg gccggcattc tgcaaaagcg gctgtttgtc gagggcagcg
     1861 atgtcagggc cggtcaagcc ctgttccaga ttgatgccag cccgtatgaa gccaccttgg
     1921 ccagcgcgca ggccagcgtg gccaaggccg aagccaacct gatgcaggcc agcgcacagg
     1981 ccgaacgatt caagccgctg atcgaggcca aagccatcag ccagcaggag ttcatcaacg
     2041 ctcaggcagc gcaaaagcag gcccaggcgg acgtggccgt ggcccgcgcg gctgtgcaaa
     2101 cagcgcgcat caatgtggct tatgccaaca tcaccgcacc gattgccggt cgcattggcc
     2161 gggcactggt gaccgaaggc gcgttggtgg gccagggtga agcgacccag cttgcggtga
     2221 ttcagcaaat cgatccgatc tacgtcaact tcacccagtc agccgctgag gtgatgaagc
     2281 tgcgcgaggc gatggaatct ggccagctca agcgcgccgg cagccagggt gccagcgtga
     2341 ggctgctgct ggaagacggc cggctctacc cgcgcgctgg caagctgctg ttttctgacc
     2401 tgacggtcga cagcaccagt ggccagatca ccttgcgcgc cgaaatgccc aacccgggcg
     2461 gcacgttgct gccaggcttg tttgtacggg tgcgcctgga gcaggcgcag gccagcaacg
     2521 ccatcagctt gccgcagcaa gccgtgacac gctcgcccca gggcgactcg gtgatggtgg
     2581 tgacacccga aggccaggca gtggcgcgcc cggtcaagat tggcgggcag caaaacggtc
     2641 aatgggtgat cctgagcggc ctgcaagccg gggagcaggt gatggttgat ggcttccaga
     2701 aactgcgcgg cccggcaccg gtgaaggcgg tgccatggca agccaactcg cctgccaaag
     2761 cggccagtgc atcagcaggc gcaagcgcgg ccaccagtgc cgtggccgcg acaagcgcct
     2821 ctgtcccggc cagcgccgct gtcagcgcgg cgcgctaagg acttttcaca atggctaaat
     2881 ttttcattga gcggcccatt tttgcctggg tgattgcgct attcatcatt gtcatgggtg
     2941 cggtttccat cagccagttg ccgattgccc agtacccgcc ggtggcacca ccgtcgatcg
     3001 tgatttcggc cacctatccg ggtgcctcgg cgcaaacgct ggaagacagt gtgctgtcca
     3061 tcgtcgagca ggaaatgaac ggttcgcccg gcctgatcta tatggaatcg gtggcgcaag
     3121 ccaatggcac cggcagtatc acgctgagtt tccagcccgg caccagcccc gatctggcac
     3181 aggttgatgt gcaaaaccgc ctgtcgcgcg ccaccccccg gctgccctct gccgtcacgc
     3241 aacaaggggt gcgggtggaa aaatcgcgca gcaacttcct gctgtttgcc attttgtcgt
     3301 cagacgaccc ggccttgaac ccggtggcgc tgggtgacta cgcctcgcgc agcatcgtgc
     3361 ccgaaatcca gcgtctgccc ggtgtcggcc aggtgcagtt gtttggcacc gagcgggcca
     3421 tgcgcatctg gattgacccg gccaaattgc tcggcttcaa cctgtcagcg gccgatgtca
     3481 ccgccgccat ccgggcgcaa aacgcccagg tttctgccgg tgaaatcggc agcctgccca
     3541 atgtggcggg tcagggcatt tcggccacgg tggtggtcaa tggtcagctt ggaactgtcg
     3601 agcagtttgg taatgtgacc ctgcgcgcca atgcagatgg ctccagtgtg cggctcaaag
     3661 acgtggcgcg catcgagctc ggcgcgcaaa cctacgccac ctcggcccga ctcaatggca
     3721 agccgtcgac cggtgttggc gtgcagttgt cacccagcgg caatgcgttg gcgaccgcca
     3781 aggcggtgcg tgcgcgcatg cagcaactcg agaagttctt cccaaaaggc atgagctgga
     3841 ccattcctta cgacagttcg cgctttatcg aaacctcttt gcgccaggtc gcagagacgc
     3901 tggttgaggc gatcgtgctg gtgtttctgg tgatgttttt gttcatgcag gactggcgct
     3961 acacgctgat tccgacgctg gtggtgccgg tggcactgct gggcaccttt gccacgctgc
     4021 tgggcctggg gttttcgatc aacgtgctga ccatgttcgg catggtgctg gtgatcggca
     4081 tcgtgg
//
LOCUS       DAOSLL010000453         4081 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19336_length_4081_cov_5.464978, whole genome shotgun sequence.
ACCESSION   DAOSLL010000453 DAOSLL010000000
VERSION     DAOSLL010000453.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19336_length_4081_cov_5.464978"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1680
                     /gene="ubiB"
                     /locus_tag="PLB25_16870"
     CDS             115..1680
                     /gene="ubiB"
                     /locus_tag="PLB25_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HOE43281.1"
                     /translation="MRRLFRGVYIVWVLLRFGLDELVLTSFNNPWLARIARIVTVGRK
                     LNAPRGQRIRQALESLGPIFVKFGQVLSTRRDLLAVDIADELALLQDRVPPFPSEVAI
                     GIIERAFKKKVDDIFVSFERQPVASASIAQVHFAVLKIRDGSHRDVAVKVLRPGMLEV
                     IDKDMDLLRMMAGWVESLSHDGKRLKPREVVAEFDKYLHDELDLVREASSAAQLRRNM
                     ADLELVLIPEMFWDYCMPDVMVMERMKGVPINQVERLRTAGVDIPKLARDGVTIFFTQ
                     VFRDGFFHADMHPGNIQVSLDPDTFGRYISLDFGIVGTLTEIDKEYLAQNFTAFFRRD
                     YKRVAELHIESGWVPASTRVDELESAVRTVCEPYFDRPLKELSLGMVLMRLFQTSRRF
                     QVEIQPQLVLLQKTLLNIEGLGRQLDPDLDLWHTAKPFLEKWMLNQIGPKKLMDELRN
                     EGPRYAKLIPELPRLLFSFLQNNSRGRGDDLQALLAEQKRTNRLLSAIVYGGLGFVLG
                     LVVMQIMVRVRIF"
     gene            1773..2102
                     /locus_tag="PLB25_16875"
     CDS             1773..2102
                     /locus_tag="PLB25_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOE43282.1"
                     /translation="MPIYAYKCNSCGFAKDVLQKMSDSQLTECPACGKSSFSKQLTSA
                     GFQLKGSGWYATDFKGGNASAAVAEPAATDAPTTAPAGPAESTSPKTDVAPVAAAGCA
                     SACACHN"
     gene            2128..2763
                     /locus_tag="PLB25_16880"
     CDS             2128..2763
                     /locus_tag="PLB25_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HOE43283.1"
                     /translation="MKKYLLTGLLVWLPLAITIWVLLWLMGLLDGIFVALLTAMQAVS
                     PEPVSNFWEQFKHIPGLGVVLVFGGLLLTGALVSNVAGRWWINHWHNLLAKIPVFRSI
                     YNSVKKVSDTLFSSNGNAFRTALLVQYPRAGTWTIAFQTGSPGGEVARHLGDDFVSVY
                     VPTTPNPTSGFFLMLPRADVIELKMSVDEALTYVISMGSVAPTTLTNVALK"
     gene            2812..>4081
                     /gene="aspS"
                     /locus_tag="PLB25_16885"
     CDS             2812..>4081
                     /gene="aspS"
                     /locus_tag="PLB25_16885"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HOE43284.1"
                     /translation="MVMRTHYCGLVTEALLGQSVTLCGWVNRRRDHGGVIFVDVRDRE
                     GYVQVVCDPDRAQTFKVAEDLRNEFCIQVKGVVRARPEGTANENLKSGKIEVLCHELV
                     VLNPSVTPPFQLDEENLSETTRLTHRVLDLRRPYMQNNLMLRYRVSMEVRKFLDANGF
                     VDIETPMLTKSTPEGARDYLVPSRVHDGHFFALPQSPQLFKQLLMVAGFDRYYQITKC
                     FRDEDLRADRQPEFTQIDIETSFMEEQGIRDMAQGLIKTVFLNTIKVDLGEFPVISYQ
                     EAMHRYGSDKPDLRVRLEFAEVTDAMTTVDFKVFSAAANMKGGRVVALKVPGGARETG
                     GMSRGEIDAYAEFVKIYGAKGLAYIKVNDKAKGRDGLQSPIVKNLHDAALNAVLERTA
                     AQDGDLIFFGSDKAKIVNDSIGALRIKIGHS"
BASE COUNT          849 a         1076 c         1191 g          965 t
ORIGIN      
        1 catcctgggt gatgtgccag cccataccct aagcaacatg gcccaaagcc tggtgactgg
       61 cttgcggcag tttgccgcca aggcagtttc cctcgttcct ggcaacgcgc cccaatgagg
      121 cgtctctttc gcggtgtcta catcgtctgg gtgctgctac gctttggtct ggatgaactg
      181 gttctgacaa gtttcaacaa tccctggctg gcgcgcattg cgcgcatcgt gacggttgga
      241 cgcaagctca atgccccgcg cggccagcgc atccgccagg cactggaaag cctgggtccg
      301 atttttgtca agtttggcca ggttttgtca acccggcgcg atttgcttgc agtcgatatt
      361 gccgacgaac tggcgctgct gcaagaccgg gttccaccgt ttccaagtga agtcgcgata
      421 ggcatcatcg agcgggcgtt caagaaaaag gtggacgaca tctttgtgtc gtttgaacgt
      481 cagccggtgg ccagcgcatc gattgcgcag gtgcattttg ccgtgctcaa aatccgtgat
      541 ggcagccacc gtgatgtggc ggtcaaggta ttgcgccctg gcatgctcga agtgattgac
      601 aaggacatgg acctgctgcg catgatggcg ggctgggtcg agagcctgtc gcacgatggc
      661 aagcgcctga agccacgcga ggtggttgcg gagtttgaca aatacctgca cgacgagctc
      721 gatttggtgc gagaggcttc cagtgccgct cagttgcggc gcaacatggc agatctggaa
      781 ctggtgctga tcccggagat gttttgggat tactgcatgc ccgatgtgat ggtgatggag
      841 cggatgaaag gcgttcccat caaccaggtc gagcgtttgc gcacggctgg ggttgacatc
      901 cccaagctgg cgcgtgacgg cgtcaccatt ttttttaccc aggtgtttcg cgacggtttc
      961 ttccacgccg acatgcatcc gggaaacatc caggtgagtc tggacccaga cactttcggt
     1021 cgctatattt cgcttgattt cggcattgtg ggcaccttga ccgaaatcga caaggaatac
     1081 ctggcgcaga atttcacagc ttttttccgg cgcgactaca agcgcgtggc tgaactgcac
     1141 atcgagtcag gctgggtgcc ggccagcacc cgtgtcgatg agctggagtc ggcggtacgc
     1201 acagtttgcg agccctattt tgatcgccca ctcaaggagt tgtcattggg catggtgttg
     1261 atgcgattgt ttcagacatc aaggcgtttt caagtcgaga ttcagccgca attggtattg
     1321 ctgcaaaaga ccctgctcaa tattgaaggt ctgggtcgcc agcttgaccc ggatcttgat
     1381 ttgtggcaca ccgccaagcc gtttctggaa aaatggatgt tgaaccagat cggaccaaaa
     1441 aaactgatgg atgagcttcg caacgaaggc ccacgctacg ccaaactgat acccgaattg
     1501 ccgcgcttgc tattttcttt cctgcaaaac aactcgcgtg gccgtggcga cgatttgcag
     1561 gccttgctgg ccgagcaaaa aaggaccaat cgactgctgt ctgccattgt ctatggtggc
     1621 ctgggtttcg tattgggtct ggtggtgatg cagatcatgg tacgggtacg gattttctag
     1681 cggaaaacgg taaagaggtc tgcaatctgc ggctacgcag ggtccgactt tataatcgaa
     1741 ggctttgcca ctctttctgg aacttgaaca ccatgccaat ttacgcctac aagtgcaatt
     1801 cctgcgggtt tgccaaggat gtgttgcaaa aaatgtcgga ttcccagctt accgagtgcc
     1861 ccgcttgcgg caagtcttca ttttccaaac aattgacatc tgccggtttt cagctcaagg
     1921 gttcgggctg gtacgccacg gatttcaagg gtggcaacgc cagcgccgca gttgcagaac
     1981 cggcggcgac agatgccccc accacagcac cggcaggccc ggccgagtca acttcgccca
     2041 aaaccgacgt agcgccagtt gcagccgctg gttgtgccag cgcttgcgcc tgccacaact
     2101 gacacatcac cgaaagttct tgtgcccctg aaaaaatacc tgctaacagg cctgctggtc
     2161 tggttgccat tggcaatcac catttgggtg ttgctgtggt tgatgggttt gcttgacggt
     2221 atctttgttg ctttactgac ggccatgcag gcggtgtcac ccgagccagt ttcgaatttc
     2281 tgggaacagt tcaagcacat tcccggcttg ggtgtggtgc tggttttcgg cggtttgctg
     2341 ttgactggag ccctggtttc caacgtggcg gggcgctggt ggatcaacca ctggcacaac
     2401 ctgctggcca agattccggt gttccggtca atttacaaca gtgtcaagaa ggtttctgac
     2461 acgctgtttt ccagcaatgg caatgctttt cgcaccgcct tgctggtgca gtacccgcgc
     2521 gcaggcacat ggacaattgc cttccagacc ggctcacctg gcggtgaggt tgcgcggcac
     2581 ctgggcgatg attttgtcag tgtctatgtg ccgaccacgc ccaacccgac cagcgggttc
     2641 ttcctgatgc tgccgcgcgc tgacgtgatt gagctcaaga tgagtgtgga tgaagccttg
     2701 acctatgtga tttcgatggg ctcggtggct cctacaacct tgacaaatgt tgcccttaaa
     2761 tgatttttga caaacccgct gccttcgatg gcggccactg aaagcagttt tatggtgatg
     2821 cgtactcatt attgtggtct ggtgacagaa gccctgctgg gccaaagcgt caccttatgc
     2881 ggctgggtca accgtcggcg ggaccatggc ggggtgattt tcgttgacgt gcgtgaccgc
     2941 gaaggttatg tgcaggtggt atgtgatccg gaccgtgcgc agaccttcaa ggttgcggaa
     3001 gacctgcgca acgagttttg catccaggtc aagggtgtcg tgcgcgcccg cccggaggga
     3061 acagccaatg agaatctgaa aagtggcaag atcgaagtgt tgtgccatga acttgtggtg
     3121 ctgaacccct cggtcacccc accgttccag cttgacgaag agaatttgag cgaaaccacc
     3181 aggctgacgc atcgtgtgct ggatttgcgc cgcccctaca tgcagaacaa tttgatgctg
     3241 cgctaccgcg tgtcgatgga agtgcgcaag tttctcgatg ccaatgggtt tgtcgatatt
     3301 gaaacgccga tgttgaccaa aagcacgcct gaaggtgcgc gcgactacct ggtgcccagc
     3361 cgtgtccatg acggtcattt tttcgccttg ccgcagagtc cccagttgtt caagcagttg
     3421 ctgatggtgg ctggctttga ccggtattac cagatcacca aatgcttccg tgacgaagac
     3481 ctgcgcgctg accgacagcc cgagttcacc cagattgata tagaaacctc gttcatggaa
     3541 gagcagggca ttcgcgacat ggctcaaggc ttgatcaaaa cggtgttctt gaacaccatc
     3601 aaggttgatc ttggcgagtt tccggtcata agctaccagg aggccatgca tcgctatggc
     3661 tctgacaagc ccgatttgcg tgtcaggctt gaatttgctg aggtcacgga cgcgatgacg
     3721 acggtcgatt tcaaggtgtt ctccgctgcc gccaacatga aaggtggtcg tgtagtggcg
     3781 ctcaaagtgc ctggcggtgc ccgcgaaacc ggcggtatga gtcgaggaga aattgatgcc
     3841 tatgcggagt ttgtcaagat ctatggcgcc aagggactgg cctacatcaa ggtcaatgac
     3901 aaggccaagg ggcgtgatgg cctgcaaagc ccaatcgtca agaacctgca cgatgcagcg
     3961 cttaacgccg ttctggagcg caccgccgca caagacggcg acctgatttt ctttggctca
     4021 gacaaggcaa aaattgtcaa cgactcgatc ggtgccttgc gcatcaagat cggtcacagt
     4081 g
//
LOCUS       DAOSLL010000454         4063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19442_length_4063_cov_5.139721, whole genome shotgun sequence.
ACCESSION   DAOSLL010000454 DAOSLL010000000
VERSION     DAOSLL010000454.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19442_length_4063_cov_5.139721"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..649)
                     /locus_tag="PLB25_16890"
     CDS             complement(170..649)
                     /locus_tag="PLB25_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOE43285.1"
                     /translation="MAFGMKARALVLDTNIVLDLLVFHDPATVPLRQALLKKDLHWIA
                     TRAMRDELERVLDYPQIVRRMEPGSQWGRATAGDAAGAVMPSAGAIARQILDSFDAQT
                     SIVPAAPKATVSCRDPDDQKFIDQAVAHQALLLSKDKAVLCMRKRLLTFGVLASVAI"
     gene            complement(985..2403)
                     /locus_tag="PLB25_16895"
     CDS             complement(985..2403)
                     /locus_tag="PLB25_16895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HOE43286.1"
                     /translation="MNKLQLFLPCAAGVEDYLLPEVQRITGRGAADVIKQRGGVALRA
                     AWRDVMLLNLHSRLAQRVLVLLSYTEYRNEQDLYRAASEVAWEVWFTPRESIKVEITA
                     QHSPLNSLNFAALKIKDALCDRFRVKAQGVRPDVNTQWPDARIYAHLTTDTCSLYIDT
                     SGEPLFKRGWREDKGDAPLKETLAAAMLAATGWLDGDADLQPLYDPCCGSGTILIEAA
                     QMACNIAAGSLRHFAFEKYLPFQKHVWGAIKKEATDAVVMPSAGQKPIIFGSDVAHRM
                     VDFAQRNAARAGVAAVIEFRGGDALQRLAPIDAASNGGGIMVLNPPYGERIEVAGVAR
                     TEHGRATGRLPLHGSRSGVAPFAKVGPAGVGYEDDAADASDATGQIGRFGARELPQMA
                     SDGEFFSQLATHWKKHYAGWTAWVLTPDLKLPGKMRLKESRRVPMWNGPIECRLFRFD
                     MVAGSARPLPGPKAPGIPHKVG"
     gene            complement(2400..3989)
                     /gene="rpoN"
                     /locus_tag="PLB25_16900"
     CDS             complement(2400..3989)
                     /gene="rpoN"
                     /locus_tag="PLB25_16900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HOE43287.1"
                     /translation="MKQGLSLRISQHLALTPQLQQSIRLLQLSTLELGQEVDQMLDDN
                     PFLERSVVEAERESFGLAQSDAPVSRSDQELEVTTYSGADYADGTRAKAENDTEETGF
                     QADDSPSWDGDGSVSLGPDDSEWGGEARAKGNNLSSDDEMDATELARNQESLQSFLHR
                     QALALRLSAEDSAALRFLIESLNDDGYLEDDLDQLARGLAGDDPQQIEQLVHHFTVAL
                     GLLQSLEPTGVAARNLAQCLSLQLKAIQRNADSTAEPAVVAAALRICEQPMDLLARRD
                     IKHLVPLCGASDTTVRAAITLIGRLEPKPGRRFVDVERNVIVPDVLVLKVGSGALVKF
                     QVRLNPEVMPRLRVHDIYANALKGHKAGGSDAANVLALQQRLQEARWFIKNIQQRFDT
                     ILRVSSAIVERQKSFFVHGELAMRPLVLREIADELGLHESTISRVTTAKYMATPFGTF
                     ELKYFFGSGLGTESGGNASSTAVRALIKQFVAAESPKKPLSDNQISDMLKEQGIECAR
                     RTVAKYREGLRIAPASLRKAL"
BASE COUNT          749 a         1265 c         1222 g          827 t
ORIGIN      
        1 atagcctttc cttgcgaatt acttacgcta aagcccggtt aggcacaagg cggtcacgcg
       61 tactgatcgc ctgagtgcga gccttgtgtg ttttggcctg gcgtggcacg gacctgccag
      121 attttgctga agttcgccgg gttgtttgtt accagcaaag tgctatcatt taaatagcta
      181 ccgacgcaag tacaccaaag gttagaagcc tttttctcat gcaaagtacg gctttgtcct
      241 tgctcagcag cagcgcctga tgtgccaccg cctggtcgat gaacttctga tcatcggggt
      301 cgcgacagct cactgtggct ttaggggcgg cgggcacgat gctggtttgc gcgtcgaagc
      361 tgtccagaat ttggcgcgca atggcaccag ccgatggcat cacagcaccc gctgcgtcgc
      421 cagccgttgc ccggccccac tggctgcctg gttccattcg cctgacgatc tgcggatagt
      481 ccagcacccg ctccagttcg tcgcgcatcg cgcgggtggc gatccagtgc agatcctttt
      541 tcagcaaagc ctggcgcagc ggcacggtgg ccggatcatg aaagaccagc aggtccaaca
      601 cgatgttggt gtcaagcacc agcgcgcgcg ccttcatccc aaaggccatg acaggcgcgc
      661 caggatggtc gatgcaccag cgctgcaatt gaaccgccaa tttgtcgcag tgccacgacg
      721 actcgggaaa cccggatgcc ttgccgggcg tggatcaggt cgtgtgagac tggcacaggt
      781 caaagcgggg ctcatggcga cttcgcgatg gccgtgacag cgggcatcca gtagccgacc
      841 gaccatactg cgcattggct gcgaaccgac caaggcagcc cattttgcag cggcaatttg
      901 gctcaccggg ctttgggcca gcaagcccgg cgcccctggc tggggtcgat tctggctgcc
      961 cggtgcataa agtttgatcg gctgctagcc gaccttgtgc ggaatgcctg gcgctttggg
     1021 acctggcagc gggcgggctg aaccggccac catgtcaaat cggaacaggc ggcattcgat
     1081 cgggccattc cacattggga cacgacggga ttctttcaga cgcatcttgc ccggcagttt
     1141 caggtccgga gtcagcaccc aggcggtcca gccggcgtaa tgcttcttcc aatgagtggc
     1201 caactgactg aagaactcac cgtcgctggc catttgcggc aactcgcgcg cgccaaagcg
     1261 accgatttgc ccggtggcat cgctcgcatc ggccgcgtca tcttcatagc caacaccggc
     1321 cgggccgacc ttggcaaatg gtgcgacacc agaccggctg ccgtgcagcg gcaatcgccc
     1381 ggtcgcccgg ccatgctcgg tacgcgccac accggcgacc tcaatgcgct cgccatacgg
     1441 tgggttcagc accatgatgc cgccaccatt gcttgccgca tcgatgggtg ccaagcgctg
     1501 caaggcatca ccaccgcgaa actcgatcac cgctgcaacc ccggctcggg cggcgttgcg
     1561 ctgcgcaaaa tccaccatgc ggtgcgccac atcgctccca aaaataatgg gtttttggcc
     1621 tgcagagggc atcacgactg cgtcagtagc ttcttttttt atagcgcccc agacgtgctt
     1681 ctgaaacggc aagtatttct cgaaagcaaa atggcgcagg ctgcccgctg cgatgttgca
     1741 ggccatttgc gcggcttcga tcaagatcgt gccgctgcca caacaggggt catacagcgg
     1801 ctgcaggtcg gcgtcaccat ccagccagcc ggtcgcagcc agcattgccg cggccagggt
     1861 ttccttcaaa ggtgcgtcgc ccttgtcttc acgccagccg cgcttgaaca gcggctcgcc
     1921 cgaggtgtcg atgtagagcg agcaagtgtc agtggtgagg tgcgcataga tgcgggcatc
     1981 gggccactgg gtgttgacat cgggacgcac gccctgggcc ttgacacgaa agcggtcgca
     2041 cagtgcgtcc ttgattttga gcgcggcaaa gttcaggctg ttgagcgggc tgtgctgggc
     2101 ggtgatctcg accttgatgc tttcacgcgg ggtaaaccag acctcccagg ccacctcact
     2161 ggcggcacgg tacaggtcct gctcattgcg atactcggtg tacgacagca gcaccaaaac
     2221 ccgctgtgcc aggcggctgt gcaggttgag cagcatcaca tcgcgccatg cggcgcgcag
     2281 cgccacgcca ccgcgctgct tgatgacatc ggcagcgccg cgcccggtga tgcgttgcac
     2341 ttcgggcagc aggtagtctt cgaccccggc cgcgcagggc aggaacaact gtagcttgtt
     2401 cacagggctt tccttagact ggccggtgca atgcgcaggc cttcgcggta cttggcgacg
     2461 gtgcgcctgg cgcattcgat gccctgctcc ttgagcatgt ccgatatctg gttgtcgctg
     2521 agtggctttt tcgggctctc ggctgcgaca aactgtttga tcagggcgcg caccgcggtg
     2581 cttgacgcat tgccgcccga ttcggtgccc agccccgagc caaaaaagta cttcaactca
     2641 aaggtgccaa acggggttgc catgtatttc gcagtggtga cccggctgat ggttgactcg
     2701 tgcagaccca gttcgtcggc gatttcgcgc aataccagtg ggcgcatggc cagttcaccg
     2761 tgcacaaaaa agctcttttg ccgctccaca atcgctgagc tgacccgcaa aatggtgtca
     2821 aagcgctgct ggatattttt gatgaaccag cgtgcttcct gcaaacgctg ctgcagcgcc
     2881 agcacattgg cggcatcgct gccaccggcc ttgtggcctt tgagcgcgtt ggcataaatg
     2941 tcgtgcaccc gcaggcgcgg catcacctca gggttcagcc gcacctggaa tttgaccagt
     3001 gcgccgctgc cgaccttgag caccagcaca tcgggcacga tgacgttgcg ctcgacatcg
     3061 acaaaacgcc gccctggctt gggctccagt cgaccaatca gggtgatggc ggcgcgcact
     3121 gtggtatcgg aggccccgca cagcggcacc aggtgcttga tgtcgcggcg cgccagcaga
     3181 tccatcggtt gttcgcaaat gcgcaatgcc gctgccacga ccgccggttc ggctgtcgaa
     3241 tcggcattgc gctgaatcgc cttgagctgc aggctcaggc actgtgccag attgcgcgcc
     3301 gccacaccgg ttggctccag gctttgcagc agccccaaag cgacggtgaa atggtgcacc
     3361 agttgttcga tttgctgcgg gtcgtcgcct gccaggcccc gtgccagttg gtccagatcg
     3421 tcttccagat aaccgtcgtc attgagcgac tcaatcaaga accgcagtgc cgcgctgtcc
     3481 tcggcactga gccgcaaggc cagcgcctgg cggtgcagaa agctttgcag cgactcctgg
     3541 ttgcgtgcca gttcggtggc atccatttcg tcgtcgctgc tgaggttgtt gcccttggca
     3601 cgcgcttcgc caccccattc gctgtcatcg gggcctaagc tgacgctgcc gtcaccgtcc
     3661 caactgggac tgtcgtctgc ctgaaaacct gtttcttcag tatcattttc ggccttggcc
     3721 cttgtcccat ctgcgtagtc agctcctgaa tatgtagtga cttcaagctc ctgatcagac
     3781 cgactgaccg gcgcatcaga ctgggccaga ccaaaggatt cgcgctcggc ttcaaccacg
     3841 ctgcgttcca gaaacgggtt gtcgtcgagc atttggtcaa cctcttgccc cagttccagc
     3901 gtggagagct gcagcagccg gatcgattgc tgcaactgtg gcgtgagtgc cagatgctgc
     3961 gaaatccgca gtgacagacc ttgtttcata gcgccgccag gccagccccc tcggggggca
     4021 gcgcagtaca cgaagtgaca agcgtggggg tcatcggccg acg
//
LOCUS       DAOSLL010000455         4063 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19444_length_4063_cov_4.475299, whole genome shotgun sequence.
ACCESSION   DAOSLL010000455 DAOSLL010000000
VERSION     DAOSLL010000455.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19444_length_4063_cov_4.475299"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2053)
                     /locus_tag="PLB25_16905"
     CDS             complement(<1..2053)
                     /locus_tag="PLB25_16905"
                     /inference="COORDINATES: protein motif:HMM:NF019237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1566 domain-containing protein"
                     /protein_id="HOE43288.1"
                     /translation="MKKQNEFWGAVTNMAIWLRWLAVVCLFAGAASSAFAAKTYTDNG
                     DGTVTDPTTGLQWMRCSMGQTWDGTTCTGTASTYTWDQANALSNMVTFAGYSDWRLPN
                     IRELQTIVDRSVFGPAIDSGTFPNAPNWNFWSNSPSARSLSFDSSIALSANFNYGESS
                     ASSKSYPLPVRLVRAGMPLGLLDIGRPNTDYVDQVNGTVSHTPTRLVWQRCVNGQSWV
                     DGTCSGTATTHTWDAAKQLTSTLAGHTDWRLPTLEELFSLVDYNRYSPALSPSLFPNT
                     PDSFFWSASVNASNSSGAWSIHFADGYALVDLSIGNAFHVRLVRAGQCLGPLVLTVSK
                     IGAGQVNSTARPGVLCNPLSGSGFYADDVVNLVAIPAANFISWGASGACSGTNPTCTV
                     TMDAAKSVTANFLDTPLISGLPSALSFALQNIGSTSSAQQITLSNTGTTALSGLSFVA
                     STEYAVTHNCGIGLGSGGNCTLNVTFRPTGTGTRSGSITISSNAPGTPHSIALSGTGQ
                     GSTSVASPTSLSFGNQGIGTTSPAQTVTLSNTGGAVLNITSKVANGDFAISSSTCGAT
                     LAPASSCIISVTFKPNTLGAQISNLVISSDAISSPTTTVTLNGTGTAVPLASLNPISL
                     IVPAQTVNTQSTEKTVVLTNTGGAALTLAAGSITASAEFAVTNNCGGGLGSGGFCNLS
                     VF"
     gene            2227..2499
                     /locus_tag="PLB25_16910"
     CDS             2227..2499
                     /locus_tag="PLB25_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43289.1"
                     /translation="MPSNNHYRTAASAAKFAGRIAEHSAINLARFATTDHTGSSKFLA
                     NLPSLGFVDTFTIVAAHIFATILGAFVSGFLMFLLIAYGIPALLML"
     gene            2792..3127
                     /locus_tag="PLB25_16915"
     CDS             2792..3127
                     /locus_tag="PLB25_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43290.1"
                     /translation="MQIIRKPADDVSDPDLRQLIDRVFATVIDCPEILGFILIVEPGD
                     TIAMLDAKLGFSILSGRHECILEHADWFELVYVLGQDGSGLEVIIPKSIDIPELLTMC
                     QQHATPAGF"
     gene            3238..3495
                     /locus_tag="PLB25_16920"
     CDS             3238..3495
                     /locus_tag="PLB25_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43291.1"
                     /translation="MGITDIRSYFQKWRIPIVISVIVCALVGAGSDGLKGFAIGGLLG
                     LVAPVALLWLGVMLTLIALFLAIYFAVWAVIVWIVWALFFR"
     gene            3618..3740
                     /locus_tag="PLB25_16925"
     CDS             3618..3740
                     /locus_tag="PLB25_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43292.1"
                     /translation="MTIYSITSTFLPKDIHPRRYRYWPAQRQMPSLERHSPTLQ"
     gene            3758..>4063
                     /locus_tag="PLB25_16930"
     CDS             3758..>4063
                     /locus_tag="PLB25_16930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43293.1"
                     /translation="MTTDTIESESVETEATDTITEVPMRVLKVATTTSLSNRSTLGYA
                     VGCDESNAIHLSLRSNTAAGMFSTSWLAFLDIAEALVHADKITSATLAPLYEGTSRNN
                     "
BASE COUNT          917 a         1165 c         1023 g          958 t
ORIGIN      
        1 aaaacacgct caagttgcaa aagccgcctg agccaaggcc accgccacag ttgttagtca
       61 cagcgaactc agcgctggcg gtaatgcttc ctgcagcgag tgtcagcgcc gctcctccgg
      121 tattggtcaa caccactgtt ttctcggtac tctgggtgtt taccgtctgt gcgggaacaa
      181 tcagactgat tgggtttagt gacgcgagcg gtacagccgt acctgtgccg ttcagagtga
      241 ctgttgtcgt aggactgctg atggcatcgc tactgatcac cagattgctt atctgcgcac
      301 caagtgtgtt gggcttgaag gtcacgctga tgatgcaact gctggccggt gccaacgtcg
      361 caccacaggt actgctgctg atggcaaagt cgccattggc aaccttgctg gtaatgttta
      421 gtactgcgcc gcctgtgttg ctgagggtaa cggtctgtgc ggggcttgtc gtgccaatgc
      481 cttgattgcc aaaactgagc gatgtgggac tagcgacact tgtactgcct tgacctgtgc
      541 cagacaatgc gatgctatgg ggtgtgccgg gggcgttact gctgattgtg atacttccgg
      601 agcgtgtacc ggtgccagtg ggtctaaacg tgacgttgag ggtgcaattg ccgcctgaac
      661 ccaagccgat gccgcagttg tgtgtgaccg catattctgt gctggcgacg aagctcaagc
      721 cagacaacgc agttgtaccg gtattgctga gggtgatttg ctgggcgctg ctggtgctgc
      781 cgatattttg cagtgcaaag ctcagtgcgc ttggcaggcc cgaaatcagt ggtgtatcca
      841 ggaaattcgc ggttactgat ttggctgcat ccatggtgac agtgcaggta gggttggttc
      901 cgctacaggc cccactggca ccccaagaaa tgaaattggc ggccgggatg gcgacgagat
      961 taaccacatc atcagcgtaa aaaccgctgc cgctcagcgg attacacaaa acgccggggc
     1021 gcgccgtgct gttcacttga cccgcaccaa ttttacttac tgtcaatacc aaagggccga
     1081 gacattggcc ggcccgcaca aggcggacat gaaaggcgtt accgatacta aggtcaacca
     1141 acgcatagcc atcggcaaaa tggatagacc aggcaccgct tgagttactg gcattgactg
     1201 aagccgacca aaaaaaactg tcgggagtat ttggaaacag cgaaggactt agggcaggtg
     1261 aatatcggtt gtagtccact aggctaaaaa gctcttccaa cgtaggcaac ctccagtcgg
     1321 tatgcccggc caacgtgctg gtcaattgct tggcagcatc ccaagtatga gtggtcgcag
     1381 tgccactgca tgtgccatca acccaagatt gcccgttcac gcaacgttgc caaaccaatc
     1441 gggtaggcgt atgcgagact gtaccgttaa cttgatcaac atagtcggtg ttcgggcggc
     1501 caatgtccag aagtcccaaa ggcattccgg cccgcacgag acggacagga aggggatagc
     1561 tcttactgct cgcgctggat tcgccgtagt taaagttagc actcaatgca atacttgaat
     1621 cgaaacttaa acttctggca ctgggtgaat tcgaccaaaa attccagttg ggagcattcg
     1681 gaaaagttcc tgaatcaatc gccgggccaa agacggatcg gtccacaata gtttgaagtt
     1741 cccgaatatt aggcagccgc cagtcactgt acccggcaaa tgtcaccatg ttggacaggg
     1801 cattggcttg atcccatgtg taagtgctgg ccgttccagt gcaagtcgta ccatcccacg
     1861 tctgccccat cgagcaacgc atccattgca atccagtggt cgggtcagtc accgtgccgt
     1921 caccattgtc cgtgtaggtt ttggccgcaa aggccgaact tgcagcccct gcaaatagac
     1981 aaaccacagc cagccagcgc agccagatag ccatatttgt gacagcaccc caaaactcat
     2041 tttgtttttt catttttatg tctcgataaa cgcagaagaa ctcggactac ggtgatcgta
     2101 gcagcccgat ttcctgagca atcaatccca gccaccattt tctttagccc cagcacccct
     2161 cgacctcacc gtccagggtg ctttttttcg cctactcatt cccctcccca acccagcagg
     2221 agatccatgc cctcaaacaa tcactaccgt accgcagcct cggctgccaa attcgctggc
     2281 cgcatagccg agcattccgc catcaacctc gcccgtttcg ccaccactga tcacaccggg
     2341 tcatccaagt tccttgccaa tctgcccagc ctgggtttcg ttgacacctt caccatagtc
     2401 gctgcacata tctttgctac catcctggga gccttcgtct caggctttct gatgttcttg
     2461 ctaatcgcct acggcatccc tgcgcttctg atgttatagc aatcaaccgc tcccgttccg
     2521 agcacccctg atccttgtga tctcgggtgc ttttttcatt ccaaaagccg cgcggctaca
     2581 agctgcttgc agctaaaaca agcctatttg gtggcgttta ccgccactgg cgacattacg
     2641 tgggcaaaaa actggaaacg tcgcaggcat atcgggcatg cctacaaatt cgcccaaacc
     2701 cttagtccat ctggtcttag gcaccccggc acccttagtc cttgtggctt tgggtgcttt
     2761 tttttcgtcc aaattttcct agaggagtca tatgcaaatc atccgtaaac ctgctgatga
     2821 cgtttccgat ccagacctgc gccaacttat cgatcgcgtc tttgccacag tcatcgactg
     2881 ccctgagatc ctgggattca ttctcattgt cgagccgggc gacaccatag ccatgctgga
     2941 tgcgaagctt ggcttttcca tcctttccgg tcgccacgag tgcattctgg aacatgccga
     3001 ctggttcgag ctggtttacg tgctcggcca agacgggtca ggactggaag tgatcatccc
     3061 caaatccatt gacattccgg aattgctgac catgtgccaa caacatgcca ctcccgccgg
     3121 tttctgacta tggcatcccg caaaccgtcc ggtctcaagc tcagccaagt gctgccaatc
     3181 tggtcgcccg ccaacacagc cgctgcaaat ttgcttttcg ttttgctgct ggagcgtatg
     3241 ggcatcacag acatccgcag ctatttccag aagtggcgga ttcccatagt catcagcgtg
     3301 atcgtatgcg ctttggtcgg cgcaggcagt gatggactga aaggtttcgc cattggcggc
     3361 ctgcttggat tggtcgcacc tgtagcgctg ctgtggcttg gtgtgatgct gaccctgatc
     3421 gccttgtttc tggctatcta tttcgcagtt tgggcagtga tcgtttggat tgtgtgggca
     3481 ctgtttttcc gatagcagat agcgcccttg ctgggccatg cgtgcattgc gcctttcagt
     3541 ccaccttata tgaataaggc ccagcctggt tcaacccctg ccaatttcat ccatccacat
     3601 ttacgaagga aaccaacatg accatctaca gcatcacatc aacttttcta cccaaggaca
     3661 tccaccctcg ccgctacagg tattggcccg cacagcgcca gatgcccagc ctcgaacgcc
     3721 attcaccaac ccttcaatga acacaaggaa aaaaatcatg acgactgaca ccatcgaatc
     3781 tgaatcagtg gaaaccgaag ccaccgacac catcaccgaa gttcccatgc gggttttgaa
     3841 agtggccacg accaccagcc tttccaaccg aagcacattg gggtatgccg ttggatgcga
     3901 cgaaagcaat gccatacacc tgagcctgcg ctcgaacact gccgctggca tgttcagcac
     3961 cagttggctg gcgtttctgg acatagccga agcgctggtt catgctgaca agataacctc
     4021 ggccacgctt gcgccactgt atgaaggaac cagccgcaat aac
//
LOCUS       DAOSLL010000456         4055 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19488_length_4055_cov_4.760250, whole genome shotgun sequence.
ACCESSION   DAOSLL010000456 DAOSLL010000000
VERSION     DAOSLL010000456.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4055)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4055
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19488_length_4055_cov_4.760250"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3683
                     /locus_tag="PLB25_16935"
     CDS             <1..3683
                     /locus_tag="PLB25_16935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE43294.1"
                     /translation="GHPIGLFTENQAVPALAGMAGGGILTLGELAQRCPTVLANPVSV
                     TQVLSHLVADRLGYLLVVNPEDASTGVIALAKLLESVRSSIHADIASHQFADSQVQWS
                     REDLKKQFDFSSAVVDAMSDGVSACTAVDQAPHVQFTVWNAAMIALTGYTMAEINRLG
                     WYQTVYCDPKVQQQARLRMERMRLGEHLVREEWVITRKNGEQRVVEISTSFVTQPGAA
                     SHVMAVMHDTTDRKRAEAELILTRNRLKSTIEALPDVMLEVDALGRILSFHLPHPNTC
                     ACFPDQMVGKKLSDIFSGDSAELMCSALEEASQHGRSVGTQCALSWLGQASWFELSIL
                     RCAQDPAELERYVILARDITSRHQIENQLRNSEANFRSFFDTIDDFLFVLDLKGSILG
                     VNKVVTERLGYEPCALIGQSVLRVHPEARRDEAGRIVAAMLAGTEDCCPVPLEAADGA
                     LIAVETKVVLGNWNGEPAIFGLSRDVTERNQINDALRDEADHRKILFDQSRDGITLLR
                     TDGSLAECNPAFAQELGYTMHELAAMHVWDWDCQHTREDLESRFARPGRQHLTLETRH
                     QRKNGSIFDVEVSVNDVEWAGQRFFFCIHQDITARKLAHEQLRESEFLLRESQRIGQL
                     GGFRADPVHNAVMWTEGIYNICELPLDYQPDLKSGLDAYLPESRELVEESLRGVLLRG
                     TPFTIQVQVRGAQTGNIKWTELRGFPHFDGQQRVDYVMGTLQDISERKRTEIALAKAK
                     EDAEAANRAKSSFLATMSHEIRTPMNGVLGMAQMLLLPNLKESERLDYARIVLSSGKA
                     LLTLLNDILDLSKIESGKFELDSNVFEPEVLLRDTCALFFGAAESKNLQLNYQWLGSA
                     GQCYRADAHRLRQMISNLVGNAIKFTPMGSVRLQAREIERDDSGSLLEFSVSDTGIGI
                     AQDKQALLFKPFSQADTTTTREFGGTGLGLAIVFNLARAMGGDVGVSSEPGHGSRFWL
                     TVRVKTIAAAQNRRNSLRPAAAQVTAGASVRHISGHVLVVEDNPVNCQVIKLLLGSLG
                     LTITLAHDGQQAVNAVTQVDPIRLPDLILMDLHMPVMDGYAATQQIRQWEQANQKLRL
                     PIIALTADAFEQDHQHCLAVGMNDFLTKPVSLDTLKMTLEQWLPSTEPPLSSCPAESE
                     PRLVNRAQVEQLLAELAPLIAKNKFAVLKRYQALQDLVAGSAAEREIRDIGRLLQTFD
                     FNLALERLHQLALLQAWKMPSS"
BASE COUNT          870 a         1112 c         1176 g          897 t
ORIGIN      
        1 gtggtcaccc cattggtctg tttaccgaga atcaggcggt gccagcgctg gcaggcatgg
       61 ccggcggcgg catcctgacg ctgggcgaac tggcgcagcg ctgcccaacg gtcttggcca
      121 atcccgtctc ggtgacccag gtgctgagcc atttggtagc tgatcgcctg ggttacctgc
      181 tggtggtcaa ccccgaggat gcatcaaccg gagtgattgc cctggcgaag ttgctcgaaa
      241 gtgttcgctc gtccattcat gcggatattg ccagtcacca gtttgccgac agtcaggtgc
      301 agtggtcgcg tgaagacttg aaaaagcagt tcgatttttc cagcgctgtg gtcgatgcga
      361 tgagtgatgg cgtttcggct tgtacggccg ttgatcaggc accacatgtt cagttcaccg
      421 tgtggaacgc tgccatgatt gcgctgaccg gctacaccat ggcagaaatc aatcggctgg
      481 gctggtacca gacggtttac tgcgacccta aagtgcagca gcaggcaagg ctgcgcatgg
      541 agcgcatgcg cctgggcgaa cacctggttc gcgaagaatg ggtgatcacc cgcaagaatg
      601 gcgagcagcg ggtggttgag atctcaacca gctttgtgac acagcccggt gccgccagcc
      661 atgtcatggc cgtgatgcac gatacgactg atcgtaaaag ggccgaagca gaattgattt
      721 tgacccgcaa tcgtctgaaa tcaacgattg aagctttgcc tgatgtgatg ctggaagttg
      781 acgcactggg gcgcattctg agttttcatt tgccacatcc gaacacatgc gcttgttttc
      841 ctgaccagat ggtcggcaag aaactgtctg acatcttttc tggcgattcc gccgaattga
      901 tgtgttcggc gcttgaagaa gccagtcagc acgggcgatc tgtcggcacg caatgcgcat
      961 tgtcatggct gggtcaggca agctggtttg aactgtcgat tctccgttgc gcgcaggatc
     1021 cggcggaact ggaaagatat gtgattttgg cccgcgacat tacctccagg catcagattg
     1081 aaaaccaact gcgcaacagc gaggccaatt tccgctcatt cttcgatacc atcgacgact
     1141 ttctctttgt ccttgattta aagggaagca tccttggtgt caacaaggtg gtgaccgagc
     1201 gattgggcta tgagccgtgt gccctgatcg ggcaaagtgt tttgcgggtt caccctgaag
     1261 cgcggcgtga cgaggccggt cgcattgtcg cggctatgct ggcgggcacc gaggactgct
     1321 gcccggtgcc actggaggct gctgacggtg cgctgattgc ggtcgagacc aaggttgttt
     1381 tgggaaactg gaatggtgaa ccggctattt ttggcctgag ccgagacgtg acggagcgca
     1441 accagatcaa tgatgcgttg cgtgatgagg cagatcacag aaaaatcctg tttgaccagt
     1501 ctcgcgacgg cataacgctg ttgcgaaccg atggcagcct ggcggaatgc aatccggctt
     1561 ttgcgcaaga gttgggctac accatgcatg aactcgccgc catgcatgtc tgggactggg
     1621 actgccagca cacccgagaa gacttggagt cccgttttgc gcgacctggt cgtcagcatc
     1681 tgacgcttga aacccgtcat cagcgcaaga acggctcgat ttttgatgtg gaagtcagtg
     1741 tcaatgatgt ggagtgggcc gggcagcgct ttttcttctg tattcatcaa gacatcacgg
     1801 cgcgcaagct ggcacacgag caactgcgtg aaagcgagtt cctgttgcgc gagagccagc
     1861 gcatcgggca gctcggagga tttcgggccg acccggtcca caacgccgtg atgtggaccg
     1921 aaggcatcta caacatctgt gaactgcccc tggactacca gcctgacctg aagtcgggac
     1981 tggatgcata cttgcccgaa tcacgcgaac ttgtagaaga aagtctgcgc ggtgttcttc
     2041 tgcgtggcac gccctttacc attcaggtac aggtcagggg tgcacagacc ggcaacatca
     2101 aatggaccga actgcgtggc tttccgcatt ttgatggtca acagcgcgtt gactatgtca
     2161 tgggcacctt gcaggacatt tccgagcgta aacgtaccga gatcgcgctg gcgaaagcca
     2221 aggaggacgc tgaagccgcc aatcgggcca aatcgtcatt cctggcaacc atgagccacg
     2281 agattcgcac gcccatgaac ggggtgctgg gcatggccca gatgctgttg ctgccaaacc
     2341 tcaaagagag tgagcggctt gactacgcgc gtatcgtcct gtcaagcggc aaggccttgc
     2401 tgacgctgct caacgacatt ctggacctgt ccaagattga atctggcaag tttgagctcg
     2461 atagcaatgt gtttgagccc gaggtgctgt tacgcgatac ctgtgccttg ttttttggtg
     2521 ccgcggaaag caaaaatctg caactcaatt accaatggct tgggtcggcc ggtcagtgct
     2581 atcgggccga tgcgcaccgc ctgcgccaga tgatctcgaa cctggtgggt aacgccatca
     2641 agttcacccc gatgggctcg gtgcgattgc aggccaggga gattgaacgc gacgacagcg
     2701 gttccctgct ggagttttcg gtcagcgata ccggtatcgg cattgcccaa gacaagcagg
     2761 cgttgctgtt caaaccgttt tcccaggccg acaccacgac cacccgcgaa tttggcggca
     2821 caggactggg gctggcaatt gttttcaacc tggcccgggc catgggtggt gatgtcgggg
     2881 tcagcagcga acctggccat ggttcacggt tctggctaac ggttcgggtc aaaaccattg
     2941 ccgccgccca gaatcggcgc aattcgctgc gaccggcagc ggcgcaagtc acagcgggcg
     3001 cttcggtccg ccacatcagc ggtcatgtgc tggttgtgga agacaacccg gtcaattgcc
     3061 aggtcatcaa gttgttgctc ggcagtctcg ggttgaccat cactttggcc catgacggcc
     3121 agcaggcggt caacgctgtc acccaagttg atccgatccg tctgcctgac ctgatcctga
     3181 tggatctgca catgccggtg atggacgggt acgctgccac gcagcagatc aggcagtggg
     3241 agcaggcgaa tcaaaaactg cgactgccca tcatcgcact caccgctgat gcgttcgagc
     3301 aagaccacca gcactgcctg gctgtcggga tgaatgattt tctgaccaaa ccggttagcc
     3361 tcgacacttt gaaaatgacg cttgaacaat ggttgcccag cacggagcca ccgctttcca
     3421 gttgccccgc cgaatcggaa ccaaggcttg tcaaccgcgc gcaagtcgaa cagctactgg
     3481 ctgagcttgc gccattgatc gccaaaaaca aatttgccgt gctcaagcga tatcaagcgc
     3541 tgcaagacct tgtggccggt agcgctgctg aaagggaaat ccgtgacatc ggccggctac
     3601 tgcaaacctt cgatttcaac ttggctcttg agcgattgca tcaactggcg ctcttgcaag
     3661 cctggaaaat gccatcgtca tgaagtcggc cagcgcctgg ttctcacccc cgttcgcagc
     3721 gccatcagtt ccccgtcgca gggcaggtcg tggcagcatc gttggttgaa cctggatcct
     3781 tctgctgacc gctagttttc atctgcaagt ccatatgagc atttacttcc ttaggcgcta
     3841 tgcgcatcaa acttaccaac atgttgtcga tctttgctga cacaacttca ccagaggctg
     3901 ttcgccgcag gcgaacctca ttttcccagc ttgtctggga aaatgcctag tcactaacaa
     3961 gtcaagtctt tgcatatcat gcgaagaatt caaacattcc aagtcattcg ccgcgagggc
     4021 gcgactccta caaattcaaa taggcgccga ctgtt
//
LOCUS       DAOSLL010000457         4053 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19503_length_4053_cov_4.409455, whole genome shotgun sequence.
ACCESSION   DAOSLL010000457 DAOSLL010000000
VERSION     DAOSLL010000457.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19503_length_4053_cov_4.409455"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..>4053
                     /locus_tag="PLB25_16940"
     CDS             27..>4053
                     /locus_tag="PLB25_16940"
                     /inference="COORDINATES: protein motif:HMM:NF017930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HOE43295.1"
                     /translation="MNWYDTQTGAPLLPMYVSTVDSGNLSAHLLAVAQACREQALCPD
                     DNGLTCQRGVAAARQRLNPLLAQRCQLAPGERNELKWLLADHRATLRSARRDQLAQAS
                     AASLGAAASTTRQRLLGTAQALQDLAWQADFSFLYSARRHLFHIGYRVAEHQRDAGFY
                     DLLASESRLTSLLAIARGDVPVRHWASLGRVFFARDAGVGLRSWTGSMFEYLMPGLVL
                     LEPWGSVLSEAGHAALREQMSFASLQGLPWGVSESAYAASDDSLAYQYAPQGVAGLAL
                     RRTPPDELVVAPYATALAAQIAPHAALLNFVGLERLGARGRYGFIEALDYTAARRTGD
                     APFVPVATFMAHHQGMSIVALANVLHQGVAQRWGMANAHLQAVSSLLHERVPREISNL
                     AAIQSGPAAQAQRRHSPGLLREVMPGSVAVEPTHLLSNGRYSVLLRANGAGSSSLGHI
                     NITRFRDDALRDERGSFVYLRRATPGAVYSVTQHPAPDPQAHYQSVFHADRVCFDAIW
                     PDLQAYTTVWVSPEDDIEFRQVELRNRSDQALQVELISAFEVCLSEARADEAHPVFGN
                     LFVSAQWLASDLALLFERKPRLATEQGLGLAHFITDASVPLSHLRHASNRLDWLGRNR
                     GANDPLAVMTGLTTSDAPVPLDTGLDPMCALSATLLVPAFAKVRVTFATAASNNPQTL
                     RAVIDKYRQTSNVQRASLMSATLIGIRLRNLRISAENLAAMQTLTSALALSLTQPQAR
                     PVRPESAATEVCDRRLLWRFDISGDRPLILVQAGVTQGLGLLRSLAQVMSLWQWGNLG
                     CDLVVVNSEPASYLMPLTRELGAWRDRHNASCSADAGPAVTRLHLLQASALSVPELNT
                     LHSLARLELRADGRPLAYHVQKWIDLHEQALVHRQDTATTALQTVRAGSTEPQRTTGE
                     FGPDTGEFVFDVQAQLRPARPWINVLANPTFGAQISEAGGGYTWAINSRLNQLTGWSN
                     DPVSDPPSEWFLLQNSRTRALWSASPSAGVQNSVTYRVVHGQGHSSISHQHGNLAVTA
                     TWCVDAHASVKQVHLTLQNHGHKTLHLRLIGLAEWLMGASRSDRSTARTALLRVPGLV
                     ALLCTQRERAAGFGDGTAFLAMTESAHDSEDWTCDRRECFDARGQLVVPDYFGQISGD
                     GLDPCAALSSHFSLGGGESAEHTFLLGYAATPVQARQLLTQAAAVSTLQRLTRVKSFW
                     QHLLGATHIKTPDALLDALTNHWLLYQTVACRVWAKAGFYQAGGATGFRDQLQDVMAL
                     AWAAPDMLRAQILVNASRQFIAGDVQHWWHAPLGAGVRTHFSDDLLWLAHACVHYLLV
                     TGDGTLLDVEQVVDRDRGRDLD"
BASE COUNT          666 a         1340 c         1309 g          738 t
ORIGIN      
        1 cgctggagcg ttaccgcggc cacttcatga actggtacga cacccagacc ggtgcgccac
       61 tgctgccgat gtacgtctcg acggtggaca gcggcaacct cagcgcgcat ctgctggccg
      121 tggcccaggc ctgccgcgag caggccctgt gccccgacga caacggcttg acctgtcagc
      181 gcggcgtggc agcagcccgg cagcggctca accccttgct ggcacaacgc tgccagctcg
      241 caccgggtga gcgcaatgaa ctcaaatggc tgctggcaga ccaccgggcg acactgcgct
      301 cggccaggcg cgatcaattg gcccaggcca gcgcagcctc cctgggcgcg gctgccagca
      361 ccacccggca gcgcttgctg ggtaccgcac aggctttgca agacctggca tggcaggcgg
      421 acttcagttt tctgtacagc gcacgcaggc acctgtttca catcggctac cgggtggcag
      481 aacaccagcg cgatgccggc ttttatgacc tgctggcatc cgagtcgcgg ctgaccagct
      541 tgctggcgat tgcccgcggt gatgtgccgg tgcgtcattg ggcgtcgctg gggcgggtgt
      601 tctttgcccg cgatgccggt gtcggcctgc ggtcgtggac cggctcgatg ttcgaatacc
      661 tgatgcctgg cctggtattg ctggagccat ggggcagcgt gctgtctgaa gcaggccatg
      721 ccgcactgcg cgaacagatg tcgtttgcca gtctgcaagg cttgccctgg ggggtttccg
      781 aatcggccta tgcggccagc gatgacagcc tggcctacca gtatgctccg caaggcgttg
      841 ccggtctggc gctgcgccgc acaccacctg acgaactggt ggtggcccct tatgccaccg
      901 cgctggcggc gcagattgca ccgcatgcgg cgctgctgaa ttttgttggc ctggagcggc
      961 tcggtgcacg cggacgctat gggttcattg aggcgctgga ctacaccgct gcacggcgca
     1021 ctggcgatgc gccgtttgtg ccggtcgcca ctttcatggc gcatcaccag ggcatgagca
     1081 ttgtggcgct ggccaatgtg ctgcatcagg gggtcgcaca acgctggggc atggccaatg
     1141 cccacctgca agccgtgtcg tcactgctgc atgaacgggt gccgcgtgaa atctccaatc
     1201 tggccgccat ccagagcggt cccgcggcac aggcccagcg ccgccattcg cccggcttgc
     1261 tgcgcgaagt gatgcccggc agcgtggcgg ttgaacccac ccacctgttg tccaacggcc
     1321 gctacagcgt gctgttgcgc gccaacgggg ctggcagcag cagcctcggg cacatcaaca
     1381 tcacccgctt tcgcgacgat gccctgcgcg atgagcgggg cagcttcgtc tacctgcgcc
     1441 gggccacgcc aggcgcggtt tactccgtca ctcagcaccc ggcacctgat ccgcaggccc
     1501 attaccaaag cgtctttcat gctgatcggg tgtgttttga tgccatctgg cctgacctgc
     1561 aagcctacac cacggtgtgg gtcagtccgg aggacgacat cgagtttcgc caggttgaac
     1621 tgcgcaatcg cagtgaccag gcgctgcagg tcgagctgat ctcggccttt gaggtttgcc
     1681 tgtcagaggc gcgcgccgac gaggcgcacc cggtttttgg caacctgttt gtcagtgcgc
     1741 agtggctggc atccgacttg gccctgctgt ttgagcgcaa gccgcggctg gccaccgaac
     1801 agggcctggg gctggcgcat ttcatcaccg atgccagtgt gccgctgagc cacctgcgcc
     1861 atgccagcaa ccggctggac tggctgggtc gcaaccgggg tgccaacgat ccgctggcgg
     1921 taatgaccgg cttgactacc agcgacgcgc cagtgcccct tgacaccggg ctggacccga
     1981 tgtgcgccct gtcggccacg ctattggtgc ccgcgtttgc caaagtccgc gtgacatttg
     2041 ccactgccgc cagcaacaac ccgcaaaccc tgcgcgccgt gatcgacaag taccgccaga
     2101 ccagcaacgt gcagcgtgct tcgctgatgt cggccacact gatcggcatc cggctgcgca
     2161 acttgcgcat cagtgctgaa aacctcgcgg ccatgcagac cctgaccagc gcgctggcct
     2221 tgagtctgac gcagccgcag gcgcgtccgg tacggcctga gagtgccgcg accgaggtct
     2281 gcgaccggcg gctgctgtgg cgatttgata tttctggcga ccgtccgctg attctggtgc
     2341 aagccggtgt cacccagggc ctggggctgc tgcgctcgct ggcgcaggtg atgagcctgt
     2401 ggcagtgggg caatctgggc tgcgacctgg tggtggttaa ttcagagcct gcctcgtacc
     2461 tgatgccgct gacccgtgag cttggcgcat ggcgtgaccg gcacaatgcc agttgcagcg
     2521 ccgacgcagg cccggcggtc acccgtctgc acctgctgca agccagcgcg ctgtcggtcc
     2581 cggagctcaa taccctgcac agcctggcgc ggctggagtt gcgcgccgat ggccgaccac
     2641 tggcctacca tgtgcagaag tggatcgatc tgcatgaaca ggcgctggtg catcggcaag
     2701 atactgccac caccgcgctg cagacggtgc gtgcgggcag caccgagccg caacgcacga
     2761 ccggtgagtt tggacccgac accggcgaat ttgtctttga cgtgcaggcg caactgcggc
     2821 cagcccgacc ctggatcaat gtattggcca acccgacctt tggcgcgcaa atatccgagg
     2881 cggggggcgg ctacacctgg gccatcaaca gccgcctgaa ccagctcacc ggctggtcca
     2941 acgacccggt gtcagacccg ccgtccgagt ggttcctgct gcaaaacagc agaaccagag
     3001 cgctttggtc ggcctcccca tcggctggcg tacaaaacag cgtgacctac cgggtcgtcc
     3061 atggacaggg gcatagcagc atcagccacc agcacggcaa cctggccgtg accgccacct
     3121 ggtgcgtgga cgcacatgct tcagtcaagc aagtccacct gacgctgcaa aaccacggcc
     3181 acaagaccct gcacctgcgc ctgattggct tggccgagtg gctgatgggg gccagccgca
     3241 gcgaccgcag caccgcccgc accgccctgc tgcgcgtgcc tgggctggta gccttgctgt
     3301 gcacgcagcg cgagcgcgct gccggttttg gcgatggcac cgcctttttg gcgatgacag
     3361 agtctgccca tgacagtgaa gactggacct gcgaccgccg ggagtgcttt gacgcacgcg
     3421 gacaactggt ggtgccagac tattttggcc agatcagcgg tgatggcctg gacccctgcg
     3481 ccgccctgtc cagtcatttt tcgctcggcg gtggcgagtc ggcagagcac acgttcttac
     3541 tgggctacgc cgccacgccg gtgcaagcgc gccaactgct gacccaggct gcggcagtgt
     3601 caacgctgca gcgcctgact cgggtcaagt cattttggca acatctgctg ggtgccaccc
     3661 acatcaaaac ccctgacgcg ctgctggatg cactgaccaa ccactggctg ctgtaccaga
     3721 cggttgcctg ccgcgtgtgg gccaaggcgg gcttttacca ggccggcggg gccaccggct
     3781 ttcgcgacca gttgcaagac gtgatggcac tggcctgggc ggcacccgac atgctgcgcg
     3841 cgcaaatcct ggtgaacgcg tcgcgccagt tcatcgccgg tgatgtgcag cactggtggc
     3901 atgcgcccct gggggccggt gtacgcaccc acttttcgga cgatctgctg tggctggccc
     3961 atgcctgcgt gcattacctg ctggtcactg gcgatggcac gctgcttgac gtcgagcagg
     4021 tcgtggatcg cgaccgtggt cgtgatctcg acg
//
LOCUS       DAOSLL010000458         4050 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19529_length_4050_cov_3.574468, whole genome shotgun sequence.
ACCESSION   DAOSLL010000458 DAOSLL010000000
VERSION     DAOSLL010000458.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19529_length_4050_cov_3.574468"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            194..1732
                     /locus_tag="PLB25_16945"
     CDS             194..1732
                     /locus_tag="PLB25_16945"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HOE43296.1"
                     /translation="MTDKLVIFDTTLRDGEQSPGASMTRDEKLRIARQLERLKVDVIE
                     AGFAASSNGDFECIKAIAEAIKDCTVCSLARASDRDISRAAEALKPANSGRLHLFLAT
                     SALHMEKKLRMTPDQVFDQARLAVRYARNLMSDIEFSPEDAYRSDPDFLCRVIEAVIR
                     EGATTINVPDTVGYAIPELYGNFIKDLRDRIPNSDKAVWSVHCHNDLGMAVANSLAGV
                     KIGGARQIECTINGLGERAGNCSLEEVVMAVKTRRDYFDLELGIDTKHIVATSRLVSQ
                     TTGFVVQPNKAVVGANAFAHASGIHQDGVLKARDTYEIMKAEDVGWSTNKIVMGKLSG
                     RNAFKLRLLELGVTMDSEADINNAFSKFKELADRKSAVFDEDILALVSEENVAQSHDC
                     YGFVSLSQHSETGERPHATVVMTVAGKEVTGQADGNGPVDASLKAIETNVRSGAEMVL
                     YSVNAISGSTESQGEVTVRLQNSGRVVNGVGADPDIVVASAKAYISALNKLQNQADRV
                     AAQG"
     gene            1834..2907
                     /locus_tag="PLB25_16950"
     CDS             1834..2907
                     /locus_tag="PLB25_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HOE43297.1"
                     /translation="MLGVHTSASGSKLGGKLRSLICLTSLFCAALLVSIPSVGHAAGS
                     KGLVNKSKKTLVVKKKHAASVVKVAKSRPVPYAAAFVTPKLSYGQIAGLHASGDSLNL
                     KSSVALVVDQDTSEVLFKKNDKAVLPIASLTKLMTGLIVSEAKLPLDEMIVISQEDVD
                     TEKGSSSRLRVGTVLSRGELLHLALMSSENRAAHALGRTFPGGISVFASQMNVKAKAL
                     GMQDTHYVEPTGLSSANQSSARDLVKLVDFAHRDPMLRELTTSPGYQLEVGSRTLQYN
                     NTNRLVKNPSWDIGLQKTGYISEAGQCLVMQAKVAGRQLIMVFLDSAGKLSRIGDAER
                     VRNWVESASGGVRLIEAKALMSH"
     gene            complement(2944..3723)
                     /locus_tag="PLB25_16955"
     CDS             complement(2944..3723)
                     /locus_tag="PLB25_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HOE43298.1"
                     /translation="MRITDSGIPAVPTVQVIERMFTLLDVLAARQDAMPLREISEKAG
                     LHPSTTHRILNDLATGKFVDRPEPGHYRLGLRLLELGNLVKNGLSVRDAALHPMRELH
                     RLTQQPVNLSMRQGDEIVYVERAYSERSGMQVIRAIGGRAPLHLTSVGKLFLAADDLQ
                     KIRTYATRTGLGGHTKNSLTQLPLLERELTKVRRDGFATDNEELELGVRCMAAGIYDD
                     QGKLVAGLSISAPTSRLEDAWLPKLKATAEEISRSLGFRPR"
BASE COUNT          967 a          948 c         1120 g         1015 t
ORIGIN      
        1 cgatgttaaa aaaaacatgt gttacattga tgccatgtac aagcactcgc tagctctaga
       61 actaccgccc gacgggtggc gttgttagcg cttgattgct tcaacccaaa agcccgtttg
      121 ccaatgcagc gggctttttt attgggcttt ggcaaatttt catgatgatt taaatttaaa
      181 caggagtaag gacatgacag ataaattggt gatttttgac accacattgc gtgatggaga
      241 acagtctcca ggtgcatcca tgacgcggga cgaaaagctg cggatcgcac gccaattgga
      301 gcgactgaag gtagatgtga ttgaggccgg gttcgcagca agctccaatg gtgacttcga
      361 atgcatcaaa gcgattgccg aggcaattaa ggattgcact gtctgttcgc ttgcgcgtgc
      421 cagtgaccgc gatatttccc gggcagcgga agcattgaag ccagccaatt cgggacgttt
      481 gcatttgttt ttggctacca gtgcgcttca tatggaaaaa aagttgcgca tgacacctga
      541 tcaggtgttc gaccaggcgc ggttggccgt ccggtacgcg cgtaacctga tgagcgacat
      601 tgagtttagt cctgaggatg cctatcggtc agatcctgac tttttgtgcc gggtcattga
      661 agcggtcatc cgggaaggtg caacaacgat caacgtgcct gacacagtgg ggtacgctat
      721 tcctgagctg tatggcaact tcatcaaaga tttgcgtgac cgtattccca acagcgataa
      781 agcggtctgg tcagtccatt gtcacaacga tctgggcatg gcagtagcga attctttggc
      841 aggtgtcaag atcggcgggg cgcgccagat tgaatgcacg atcaatggat tgggcgagcg
      901 ggccggcaat tgttcactcg aagaggtggt gatggcggtc aagacgcgtc gggattattt
      961 cgaccttgaa cttggcattg acaccaagca cattgtggca accagtcgtc tggttagtca
     1021 gacaaccggt tttgttgtcc agccgaataa agcggtcgtt ggtgctaacg cctttgccca
     1081 tgcctcaggt attcaccagg acggtgtttt gaaagcgcgc gatacctatg aaatcatgaa
     1141 ggcggaagat gttggatgga gtactaacaa gatcgtaatg ggaaagttaa gtggccgaaa
     1201 cgcatttaag ttgcgcctgc tggaattagg agtcacgatg gatagtgagg ccgacatcaa
     1261 taacgcgttc agcaagttca aggaacttgc tgatcgaaag agtgcagttt tcgatgaaga
     1321 cattctcgcc ctcgtgagtg aggaaaatgt ggctcagagc catgactgtt atgggtttgt
     1381 ttccctttcc cagcatagtg agaccggaga gaggccgcat gctactgtag ttatgacagt
     1441 tgctggcaag gaagtaaccg gacaggcaga tggcaatggg cctgtagatg cgtcccttaa
     1501 ggcgattgaa acaaatgtca ggagtggtgc ggagatggtg ctttactcgg tcaacgccat
     1561 cagcggctcc actgaaagcc agggggaagt gacagtgcgg cttcagaaca gtggtcgagt
     1621 ggtaaacggt gttggagctg atcctgacat tgttgtggca tcggccaagg cctatattag
     1681 tgcgttaaac aagttgcaaa accaagctga tcgggttgca gcccagggct agatcacaaa
     1741 aattacttta aggaagtctc gcaagtagtt gttacttcga gacatttgcg gataaactcg
     1801 cttcttcaga aatttgggta ctggagacag tctatgctgg gtgttcatac gagtgcgtca
     1861 ggaagcaaac ttggcggtaa attgcggagc ctgatctgtc taacgagtct cttctgtgct
     1921 gcgttgcttg tgtctattcc atccgttggt cacgcggccg gcagtaaggg actggtgaac
     1981 aagtccaaga agaccttggt tgtcaagaaa aaacatgctg catcggtggt caaagtcgcc
     2041 aagagtagac ctgtcccata cgccgcagct tttgtcaccc ccaaactttc atatggtcag
     2101 attgccggac ttcatgcgtc gggagacagt ctcaatttga agtccagtgt cgcgctggtg
     2161 gtggatcagg ataccagcga ggtacttttt aaaaaaaatg acaaggcggt tttaccgata
     2221 gcctcactaa ctaaacttat gaccggcttg attgttagcg aggcaaaact gccattggac
     2281 gaaatgatcg ttatctcgca agaagacgtg gataccgaaa aaggtagttc ttctcgcttg
     2341 cgagttggaa ctgtgttgag tcgaggggag ttgttgcacc ttgcgttgat gtcaagcgaa
     2401 aaccgagcgg cgcacgcatt gggtcgaacc ttcccaggag gcatcagtgt ttttgcatca
     2461 cagatgaatg tcaaggcaaa ggctctgggc atgcaggata cgcactatgt tgagccaacg
     2521 ggcttgtcca gcgccaacca atccagcgca cgcgatttag tcaaattggt cgacttcgcc
     2581 caccgcgacc ccatgttgcg cgaactgaca acatctccag gataccagct tgaagtcgga
     2641 agccggacct tgcaatacaa caacactaac cgcttggtta aaaatccgtc gtgggatata
     2701 ggtttgcaaa aaaccggata catctcggaa gccggtcaat gcttggttat gcaagccaag
     2761 gtcgccgggc gtcagttgat catggtgttc ttggattcgg ccggcaagct aagtcggata
     2821 ggagacgctg agcgtgttcg caattgggta gagtctgcca gcggtggtgt tcgcttgatt
     2881 gaggcaaaag cgctaatgtc tcattgaatc attgttttct gcgcttcaga attccaaatt
     2941 aaatcaacgg ggtcgaaatc ccaagctgcg ggaaatctcc tctgcggttg ctttgagttt
     3001 gggtagccaa gcatcttcaa ggcggctcgt cggagcagaa atcgataacc ctgcaaccag
     3061 cttgccttga tcgtcgtaaa ttccagcagc catgcatctg acacccagtt caagctcttc
     3121 gttatcggtt gcaaaaccat cccgacgaac ttttgtcagt tctcgctcta gcagaggcag
     3181 ttgtgtcaaa ctattttttg tgtgtccgcc taggccagtt cgtgttgcgt aagttctgat
     3241 cttttgcaga tcgtctgcgg ccaaaaacaa cttgccaacg gatgtcaggt gcagtggtgc
     3301 ccgccccccg attgcccgaa tgacctgcat ccctgatcgc tcgctgtatg cccgttcgac
     3361 atagacaatt tcgtcgccct ggcgcatgct taagttgact ggttgttgtg ttaggcggtg
     3421 cagctcccgc atggggtgga gcgctgcatc acgaacgctg aggccgttct taaccaaatt
     3481 gccgagctcc agcaatcgca atcccagacg atagtgacct ggctcaggcc tgtccacaaa
     3541 ctttccggta gccaggtcat tcagtattcg gtgtgttgtg gacgggtgaa ggcctgcctt
     3601 ctcgcttatt tctctaagtg gcatggcatc ttgacgcgcg gccaacacgt ccaacagggt
     3661 gaacattctt tcaatcactt gaaccgtggg cacggcaggg attcccgagt ctgttattcg
     3721 catgcaatat tctactaata tgcatgtcat tttgctatgt gaaaatccag tgttttctat
     3781 ttgctttggc gctcacggaa actgacccac ctgtgctcac agaaatctga cccactgcct
     3841 gcgcacttgt ttgttctttg gcggcccgac gcacaaacat ttcagtgacc aacttgtggg
     3901 ccggtaggcg cttgcgccgt ccttggattg ggtaattttt ttaaaacaag ccgggtgggg
     3961 acccgcgtag cggggaggcg cggctacagc gcctcggact tgtccatacg aagtgtgggc
     4021 aagtcctggt aaggtttgtc cacactgagc
//
LOCUS       DAOSLL010000459         4047 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19547_length_4047_cov_4.174098, whole genome shotgun sequence.
ACCESSION   DAOSLL010000459 DAOSLL010000000
VERSION     DAOSLL010000459.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19547_length_4047_cov_4.174098"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="PLB25_16960"
     CDS             complement(<1..296)
                     /locus_tag="PLB25_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013392320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase"
                     /protein_id="HOE43299.1"
                     /translation="MNHYNSTLPYPRDLKGYGPTPPHARWPNQARIAVQFVLNYEEGG
                     ENCVLHGDAGSEQFLSEMFNPASYPERHISMEGIYEYGARAGVWRILREFEQRGL"
     gene            410..763
                     /gene="uraH"
                     /locus_tag="PLB25_16965"
     CDS             410..763
                     /gene="uraH"
                     /locus_tag="PLB25_16965"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HOE43300.1"
                     /translation="MGLSTHVLDTMHGTPAAGMQVALFATEGGTARCLKQFVLNQDGR
                     NPDGLLLDAASLQKGTYRLEFEVAAYFKARRVALPEPNFLDRVSLDFGVAEPQQHYHV
                     PLLVSPWSYSTYRGS"
     gene            complement(1114..1641)
                     /locus_tag="PLB25_16970"
     CDS             complement(1114..1641)
                     /locus_tag="PLB25_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43301.1"
                     /translation="MKWPNLQRFRLTSLLLTISAGVAFLFGGCNSTPPSESTLPVPQP
                     GPQPTPAAVTPVVPGTVSSSQAKTPREYRKDAASHLYGKNQDRIYHGKMPPLLYAIGV
                     LQVEVDAKGSVSGIKWMRAPKHAPEVIAEIERTVRAAAPYPMPVRLGRVTYTDTWLWH
                     RTGTFQLDTLTEGQY"
     gene            1773..2630
                     /gene="xdhC"
                     /locus_tag="PLB25_16975"
     CDS             1773..2630
                     /gene="xdhC"
                     /locus_tag="PLB25_16975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0009115 - xanthine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase accessory protein XdhC"
                     /protein_id="HOE43302.1"
                     /translation="MLPVNQLLQRLARQQAVLVTVHAHRGSVPREAGAWMAVFADCTL
                     GSIGGGHLEWQAIAAARVRLEQPAPRLAPPGLVRYALGPSLGQCCGGEVHLCFEMLSA
                     ADVPMLRQRFVTQQQRWPQVAVFGGGHVGSALVNILGSLPLQVLWVDSRDGIFPETVP
                     DNVLFEHSDPVQAAVNTLAPGTQVLIMSFSHAEDLELVAACLARQRAQADLGMIGLIG
                     SKTKWATFRQRLTARGFGAQEFAHVTCPLGLPGIVGKAPPVIAVAVAAQLLLAQPVVA
                     HNSNIGESK"
     gene            2791..4032
                     /locus_tag="PLB25_16980"
     CDS             2791..4032
                     /locus_tag="PLB25_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HOE43303.1"
                     /translation="MESYYLDWANLLLRWTHVVTAIAWIGSSFYFVFLDSSLTPPVDE
                     DLKKQGVSGELWALHGGGFYHPVKFAQRPPQLPAHLHWFYWESYSTWLSGFSLFAVSY
                     LYSASTYLIDKSKMDWAAWQAISVALGFLAVFWLLYNLICRLLGQRKNGDAMVGALVL
                     LLVCLASWLACQWFASKAAFLLMGAMLATAMSANVFFWIIPGQRKMVAMIKAGQTVDP
                     IHALVGKQRSVHNTYFTLPVLFAMLSGHYSFTYLHPQNWLVLIAMMFAGAAIRQFFVM
                     RHGYKLGRHGYPLGYVLVGLAVIVALIVWLAPRSALQSPSVATTSVASDASVTGAGGP
                     IGYKILQPVLAQRCVVCHGAQVQMKNVRLDSAHNVKLHAQNIYQQAVVSKIMPMSNST
                     GISEAERMLIKQWFETGASVD"
BASE COUNT          748 a         1122 c         1293 g          884 t
ORIGIN      
        1 aggccgcgtt gctcgaactc gcgcaggatg cgccagacac cggcacgcgc gccatattcg
       61 taaatgcctt ccatgctgat atgccgctcg ggatagctgg ccgggttgaa catttcggac
      121 aaaaactgct cgctgcccgc atcgccatgc aacacgcagt tttcgccacc ctcctcgtag
      181 ttcagaacaa actgcacagc aatgcgggcc tgatttggcc accttgcatg cggtggtgtc
      241 ggaccatagc cctttaggtc gcgcgggtag ggcaaggtgc tgttgtagtg gttcatgggg
      301 ggacaaaaca gtgggaagct caggtggcat gatagctgcg tcgatcaacc tgggttcagg
      361 ccaaatacac ttgcccagta cactttgggc cgcttttgag gaaatcacca tgggactgag
      421 tacacatgtg ttggacacca tgcatggcac accggctgct ggcatgcagg tggcgctgtt
      481 tgccaccgaa ggcggcaccg cgcgctgcct caagcagttt gtgctgaacc aggatggtcg
      541 caacccggat ggtttgctac tggacgcggc cagcttgcaa aaaggcactt accggctcga
      601 atttgaagtg gctgcgtatt tcaaggcgcg ccgcgtggcg ctgccagagc ccaattttct
      661 tgaccgggta agcctggact ttggcgtggc cgagccgcag cagcactacc acgtgccgct
      721 gctggtgagc ccgtggagtt attcgaccta ccggggatcg tgacagtggc cagcttgtcc
      781 agttgggcca accaaacgct cgcttcgcgt cgctcaggtc tgtcccgcgt gcgcctgttg
      841 ccagtcgggg tggacatcca acatccgacg atcagttggg caagagacaa gctcgcttcg
      901 cgtagctcag gtctgtcccg cgtgcgcctg ttgccagtcg gggtggacat ccaacatccg
      961 acgatcagtt gggcaagaga caagctcgct tcgcgtagct caggtctgtc ccgcgtgcgc
     1021 ctgttgccag tcggggtgaa catccaacat ccgacaatca gttggggaca gagctggctc
     1081 gcttcgcgta gctcaggtct gtcccgcaat gtctcagtat tgcccttctg tcagggtatc
     1141 gagctggaag gtgccggttc tgtgccaaag ccaggtgtcg gtataggtga cgcggccgag
     1201 gcgaaccggc atcgggtatg gcgcggcggc gcgcacggta cgctcgattt cggcgatgac
     1261 ctcgggtgca tgcttgggtg cgcgcatcca tttgatgccc gaaacactgc ctttggcatc
     1321 aacctcaact tgcagcacgc caatggcgta gagcaatggc ggcatcttgc cgtggtaaat
     1381 gcggtcctgg tttttgccat acaggtggct ggcagcgtcc ttgcggtatt cacggggcgt
     1441 tttggcctgc gacgagctga ccgtgcctgg cacgaccggg gtcactgcgg ccggcgtagg
     1501 ctggggccct ggctggggca ctggcagggt cgactcggac ggcggggtcg agttgcagcc
     1561 gccaaacaaa aaagctacac cggcgctgat ggtcagcagc aggctggtca ggcgaaagcg
     1621 ttgcaggttg ggccatttca tggttcaggg tggctgcttg acagcgttgg ttgggtacga
     1681 gggcaactgg ctgagccgtt gcctatcgag cgtactgtgc ctgaaaaata cattgctgcc
     1741 gcgccggtca accttttttt acgtaagtgt ttgtgttgcc agtcaaccag ctcctgcagc
     1801 gcctagcccg ccagcaagcc gtgctggtca cggtgcacgc gcaccgtggc tcggtgccgc
     1861 gtgaagctgg tgcctggatg gcggtgtttg ccgactgcac actgggcagc attggcggtg
     1921 gtcatctgga gtggcaggcc atcgcagcgg cgcgagtccg gctcgaacag cctgcacccc
     1981 ggctggcacc gccaggcctg gtgcggtacg cgctggggcc gtcattgggg cagtgttgcg
     2041 gcggagaagt gcatttgtgc tttgagatgc tcagcgcggc cgatgtgccg atgttgcgac
     2101 agcgctttgt gacgcaacaa cagcgttggc cacaggtggc ggtatttggc ggcggccatg
     2161 tcgggtctgc gctggtgaac attctgggca gtttgccgct gcaagtgctg tgggtcgata
     2221 gccgggacgg catttttccg gaaactgtgc ccgacaacgt gttgtttgag cactctgacc
     2281 cggtgcaggc agcagtaaac accttggcac cgggtaccca agtgttgatc atgagtttta
     2341 gccatgccga agacctggaa ctggtcgccg cctgcctggc tcgccagcgc gcccaggctg
     2401 acctgggcat gatcgggttg attggcagca aaaccaaatg ggccactttc cggcagcgct
     2461 tgacggcacg cggctttggc gcacaggagt tcgcccacgt cacctgcccg ctgggcctgc
     2521 cgggcattgt cggcaaggca cccccggtga ttgccgtggc ggtagcggca caactcttgc
     2581 tggcacaacc ggtggtggcg cacaacagca acattggcga atcgaaatga gcgctgcagg
     2641 gccaaggcga acgcgtcgcc agcacggttg gtcggttaat gtgaaacaac gagcaatcga
     2701 gcaatttatg ttcaataatt ttgaacgaag aagtttttca tgatgcgggg tggccgtctc
     2761 ggcaatgaaa gttagacgaa ggacacattg atggaaagct attaccttga ctgggccaac
     2821 ctgcttttgc gctggacgca cgtcgtcacc gccatcgcct ggattggttc gtcgttttat
     2881 tttgtctttc tcgacagcag cctgacgccg ccggtcgatg aagacctgaa aaagcagggg
     2941 gtcagcggcg agttgtgggc cttgcacggt ggcggctttt atcacccggt gaagtttgcg
     3001 cagcgaccgc cgcagttgcc agcgcatctg cactggtttt actgggagag ctacagcacc
     3061 tggttgtccg gcttctcgct gtttgccgtg tcgtatttgt acagcgccag cacctacctg
     3121 atcgacaagt caaagatgga ctgggcagca tggcaggcga tcagtgtggc gctggggttt
     3181 ctggcggtgt tctggctgct gtacaacctg atttgccgcc tgctcgggca acgcaaaaac
     3241 ggtgatgcca tggttggcgc gctggtgctg ctgctggtgt gcctggcttc gtggctggcc
     3301 tgccagtggt ttgccagcaa ggcggctttc ctgctgatgg gtgccatgct tgccaccgcc
     3361 atgagcgcca atgtgttttt ctggatcatc cccggtcaac gaaagatggt ggcaatgatc
     3421 aaggccggac agacggtgga cccgattcat gccttggtgg gcaaacagcg tagcgtgcac
     3481 aacacctact tcaccctgcc ggtgctgttt gccatgctca gcggccatta cagctttact
     3541 tacctgcacc cgcaaaactg gctggtgctg atcgcaatga tgtttgccgg tgcggcgatt
     3601 cgccagtttt ttgtcatgcg ccacggttac aagctggggc ggcacggcta tccgctgggt
     3661 tatgtgctgg tggggctggc ggtgattgtc gccctgatcg tctggctggc ccccaggtcg
     3721 gccctgcaga gtccgtcagt tgctaccact tcagtagctt ctgacgcaag cgtgacgggc
     3781 gctggaggcc caattggcta taaaatcctg caacccgtgc tggcccagcg ttgtgttgtg
     3841 tgccatggtg cgcaggtgca aatgaaaaat gtccggctgg actcggcaca caacgtcaag
     3901 ctgcacgcac aaaacattta ccagcaggcg gtggtcagca aaatcatgcc catgtccaac
     3961 agcaccggca tatctgaagc tgaacggatg ctgatcaagc agtggttcga gaccggggca
     4021 agtgtggatt gaactcaatc cactttg
//
LOCUS       DAOSLL010000460         4035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19638_length_4035_cov_4.242714, whole genome shotgun sequence.
ACCESSION   DAOSLL010000460 DAOSLL010000000
VERSION     DAOSLL010000460.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19638_length_4035_cov_4.242714"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..380
                     /locus_tag="PLB25_16985"
     CDS             <1..380
                     /locus_tag="PLB25_16985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE43304.1"
                     /translation="LTAVTKTRILIVEDEVIIAMDIAMQLRELGYEPLGHATRGEQAI
                     ELVKQLQPDLVLMDVHLGNSMDGITAASSIRSQFGTKIVFLSAFDTRANRARAEAVGP
                     AGYITKPFDTGELSVAIEAAIHI"
     gene            669..1574
                     /locus_tag="PLB25_16990"
     CDS             669..1574
                     /locus_tag="PLB25_16990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HOE43305.1"
                     /translation="MTAPNFSDGVAFVQGQYVPIADASIPMTDWGFLRSDACYDVVTV
                     WDGRFFRLDAHLERFMRSCRRWRLDPGLTPESVTDVLVQCVRLSGLRVSYVEMICTRG
                     KPPWGSRDPRLAVNQFYAFAVPYVWIANEQQREIGIHLMISDVQRIPAASVDPTAKNY
                     HWNDLTMGLFGALDAGADSVLLVDALGNVVEGPGFNVFCVSHGVLVTPDQGMLEGITR
                     RTVIEMTQSLGIEVQIRTLPASELRGAEEVFISTSAGGVLPATKVDQIQIGDGLPGII
                     TRRLVQTYWDWHAKPAYTMAIDYSI"
     gene            complement(1768..2190)
                     /locus_tag="PLB25_16995"
     CDS             complement(1768..2190)
                     /locus_tag="PLB25_16995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013436190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOE43306.1"
                     /translation="MLYVDTSVLVALCTNEAKTADVVKWYSACTGELVSAAWCVTEFA
                     SAMGLKQRTGQLTQAQAQNAWLQFERLCANDLQLLSIETMTFHKAAVLTMDADAGLRA
                     GDALHLACALDAKAQGIATLDRVMAKNAKRLSLRPVVI"
     gene            complement(2190..2441)
                     /locus_tag="PLB25_17000"
     CDS             complement(2190..2441)
                     /locus_tag="PLB25_17000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019937556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HOE43307.1"
                     /translation="MMNLQIAKWGNSLAVRIPADFVRQIGVREGDLFEARLGADGALN
                     LKPPAQWSRKAFADEMARNNQVLPMGASVMEQLRQEARY"
     gene            complement(2484..3836)
                     /locus_tag="PLB25_17005"
     CDS             complement(2484..3836)
                     /locus_tag="PLB25_17005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HOE43308.1"
                     /translation="MEIKGLKMARPPKSKSPDLSESQDLTAGLIDRLTCPTGKQQAFL
                     RDCKAPGLRVRVTAAGAKSFVFEAKLNRQTIRRTIGDVRAWPIDAARIEANRLRVTLD
                     GGTDPRELELQQQAIKAADKAAAAVRAVTVAEVWAIYLDERKRHWGDRHYRDHLKLSD
                     AGGEPFKRGRGTTLAGPLAPLMPLLMPDLTAAVIEAWAANEAQTRPTQARLAWRLLKV
                     FVGWCNEQPAYAGILTGNPAKTKKARESLGRPGVKSDVLQKGQVSAWFAAVQKIGNPA
                     ISAYLQTILLTGARPGEVMQMRWDDLNVKWMGLTIRDKVEGERAIPLTPYVWHILSTL
                     TRRNEWIFSSQTSKSGHLTEPTHPHAIACKVAGLGVLTLHGLRRSFKSLTEWLEVPVG
                     VVAQIQGHKPSATAEKHYTVRPLELLAVHHNRIEAWILEQAGIVFDAKASAGALSVVS
                     "
BASE COUNT          868 a         1101 c         1076 g          990 t
ORIGIN      
        1 gcttgacagc agtgaccaaa acgagaattc tgattgtcga agacgaggtc atcattgcca
       61 tggacattgc aatgcagctc agagagcttg gttacgaacc attgggacat gcaacacggg
      121 gtgaacaggc aatcgagttg gttaaacagc tacaaccgga tctggtcttg atggatgttc
      181 acttgggcaa ttccatggac ggaatcacgg ctgcttcttc gatccgctct caatttggaa
      241 ccaagattgt cttcctgagc gcgtttgaca ccagggctaa tcgcgcccgc gctgaagctg
      301 tcggacctgc gggctatatc accaagccat ttgacaccgg tgaactgagc gtggcaattg
      361 aagctgctat tcacatttag caaagcaaat gcttctggag catgacacct gttgagcgtc
      421 cagctctcga atccccctgt gtggcgcgca ctgcttttgg tcaataaagg catcgcaaca
      481 atcagaacga gaacctggtg atcgaaaaga ttcaatgggt gaccttgtgc cccttctgac
      541 gattgcgctt gtctttgagg gcttgatgcc agatttttcc agaaaattgt cagcgctcct
      601 tatagggggc tgtgaaacac agccaaattg cataatcata aaaaatttca acgcatagga
      661 cgtggcttat gaccgccccc aatttttccg atggtgtcgc ctttgttcag gggcagtatg
      721 tgcccattgc cgacgcaagc attcccatga ctgactgggg ttttctgcgc tctgatgctt
      781 gctacgacgt ggtgacggtg tgggacggcc ggtttttccg gcttgatgcc caccttgagc
      841 gtttcatgcg aagttgcagg cgttggcggc tggacccggg cttgaccccg gagagtgtca
      901 ctgatgtgtt ggtccaatgt gtgcgcctga gtggccttcg cgtatcctat gttgagatga
      961 tctgcacccg gggcaagcca ccgtggggat cacgtgaccc acgcctggca gtcaaccagt
     1021 tctatgcctt tgccgtgccc tatgtctgga tcgccaatga gcaacagcgc gaaattggga
     1081 ttcatctgat gatcagtgac gtgcaacgaa ttccggccgc gtcggtggac cccaccgcca
     1141 agaattacca ctggaacgac ctgacgatgg gattgtttgg tgcgcttgat gcgggtgctg
     1201 acagcgtgct gctggttgat gcgcttggta atgtggtcga aggaccggga tttaacgtct
     1261 tctgcgttag ccacggcgtg ctggtgactc ccgatcaagg gatgcttgaa ggcattaccc
     1321 ggcgcacggt gatcgagatg acgcagtcgt tgggcattga ggtgcagatc agaactttgc
     1381 ctgccagtga actgcgcggg gctgaagaag tattcatatc gacctcggct ggtggtgtgc
     1441 tgcccgccac aaaggtggac cagattcaga ttggcgatgg ccttcccggc atcatcaccc
     1501 ggaggttggt gcagacttac tgggactggc atgctaaacc ggcttacacc atggcgattg
     1561 actattcaat ttgattgcac agtgaattca ggccggtttc atcggtaact cttatgcgtt
     1621 tgcaggtgtc acactcagtc gcaagacaaa agcggccagc actttgtaaa ggttgcctga
     1681 gatgggttgc tttgcgccat gggagagtct tcgattgcat ttacagcttt gatgccagtt
     1741 gagcgagttt gccaaattgt cttggtgcta aatcaccacg ggtctcaaac taagccgttt
     1801 ggcgttcttg gccatcacac ggtctaaggt cgcgatcccc tgcgctttgg catccagcgc
     1861 gcacgccaaa tgcaaagcgt caccagcccg caagccagcg tctgcatcca tcgtcaatac
     1921 ggcggctttg tgaaaggtca tggtctcgat ggacaacaat tgcaggtcgt tggcgcagag
     1981 tcgttcaaat tgcagccagg cgttttgtgc ctgcgcttga gtcagttgcc cggtgcgctg
     2041 cttgagcccc atggcactgg caaattcagt aacgcaccat gcggcagaaa ccagctcacc
     2101 cgtgcaggcc gaataccact tgaccacatc ggctgttttg gcctcattgg tacagagcgc
     2161 caccagtacg ctggtatcga cgtacaacat cagtagcgtg cctcttgacg cagttgctcc
     2221 atcaccgaag cccccatggg caacacctgg ttgttgcgcg ccatttcgtc tgcaaaggcc
     2281 ttgcggctcc actgcgctgg cggcttcagg tttagcgcac cgtcagcccc cagccgtgcc
     2341 tcaaacaagt caccctctct cacgccaatc tgacgaacaa agtcagcagg aatgcgaacg
     2401 gcaaggctat tgccccattt ggcaatctgt agattcatca tggtggctat tgatatacgt
     2461 taaagtctct acattctagt tgattacgac acgacgctca acgcacccgc cgaagccttg
     2521 gcatcaaaca caatcccggc ctgttccagt atccatgcct ctattcggtt gtgatgaaca
     2581 gcaagtaatt ccagtggccg caccgtgtaa tgcttttcag cggtcgcgct gggcttgtgt
     2641 ccttgaatct gtgccacaac gcccaccggc acctccagcc actctgtcaa tgatttgaac
     2701 gagcggcgaa ggccgtgcaa ggtaagaact cccaagcccg ccaccttgca agctatggcg
     2761 tgtgggtggg taggttcggt tagatgcccg ctcttactgg tctgacttga gaaaatccac
     2821 tcattgcgac gagtcagggt ggacagtatg tgccagacgt agggtgtcaa tggaatcgca
     2881 cgctcgcctt ccactttgtc cctgatggtg aggcccatcc acttcacgtt aaggtcgtcc
     2941 caacgcattt gcatgacttc gccagggcga gcccctgtca gcagaatggt ttgaaggtag
     3001 gcggaaatgg cagggtttcc tatcttttgc actgctgcga accatgcaga cacctgaccc
     3061 ttttgtagga catccgattt aacgccgggc cgacctaaag actctcgggc tttctttgtt
     3121 ttggctgggt tgcctgtcag gattccagcg taggcaggtt gttcgttgca ccatcccaca
     3181 aacactttca acaatcgcca tgcaagccgc gcttgtgtag gccgagtctg tgcctcgttt
     3241 gctgcccacg cttcaatcac agccgcagta aggtcaggca ttaacagcgg catcagcgga
     3301 gccagcggtc ccgcgagagt cgttccccta cctcgcttga agggttcccc tcccgcgtcg
     3361 ctcaatttca ggtgatcgcg atagtgacga tcaccccaat gccgcttgcg ttcatcaagg
     3421 taaatagccc aaacttcggc aacagtgacc gcccgcaccg cggcagcggc cttgtcggca
     3481 gctttaatcg cctgttgctg cagttccaac tctcgcgggt cggttccgcc atcaagggtc
     3541 actcgcagac ggtttgcctc gatcctggcg gcatcaattg gccaggctct gacatcgcca
     3601 attgtgcggc gaatggtctg gcggtttagc tttgcctcga atacgaacga ctttgcacct
     3661 gccgcagtta cccgcacccg taaccctgga gctttgcagt cacgcaagaa cgcttgttgc
     3721 ttgccggtcg ggcaggttag gcgatcaatt aatccggcgg tcaaatcttg ggactcgctc
     3781 aggtctggcg atttgctctt tggtggtcgt gccatcttca gccctttaat ttccatgtag
     3841 ccaccatgta gccactttga agctatttta atgcattgaa gtcaacactc gatcagcatt
     3901 ttttcctgaa attcatcgta acatattgat ttataataat tcatgcaacg acaatcaaca
     3961 ctccgaaata cattatatat cggactgtta atccgtaggt ccctggttcg agcccaggtc
     4021 gaggagccaa aaaac
//
LOCUS       DAOSLL010000461         4035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19641_length_4035_cov_3.182663, whole genome shotgun sequence.
ACCESSION   DAOSLL010000461 DAOSLL010000000
VERSION     DAOSLL010000461.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4035
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19641_length_4035_cov_3.182663"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="PLB25_17010"
     CDS             <1..342
                     /locus_tag="PLB25_17010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43309.1"
                     /translation="KWDYFVVEKSPVLTALIERRGNVDSVMLLTTKTGRAVSASMLRA
                     RYDDARIKAAAANPKLADDIKAMFLRDTRKRAADLADDVDEASKLLQHSSKALTAKHY
                     RTKGTELKAVR"
     gene            complement(657..1154)
                     /gene="mobB"
                     /locus_tag="PLB25_17015"
     CDS             complement(657..1154)
                     /gene="mobB"
                     /locus_tag="PLB25_17015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HOE43310.1"
                     /translation="MRIFGIAGHSGMGKTTLLEKLVPEIAARGLVVSLIKHSHKNIDI
                     DRPGKDSYRLRESGCMEVLLLGSDRWALMHEMRGAQEPSLDYLLDRMQNCDLVLIEGF
                     KSGGFPKLEVWRDGQSKETLWPDWPGIAVLACDTPLDGKGLPILDLADTSSIADFVLQ
                     HAVAR"
     gene            1682..1990
                     /locus_tag="PLB25_17020"
     CDS             1682..1990
                     /locus_tag="PLB25_17020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOE43311.1"
                     /translation="MKFVHILIGASVLTCAPAFASSALATQKACLACHTADKKLVGPS
                     YQEVAKKYAGQKDALAVLTKAIKTGGSGKYGPVPMPPQTALSDADAKTLAAWVLAGAK
                     "
     gene            2359..3366
                     /locus_tag="PLB25_17025"
     CDS             2359..3366
                     /locus_tag="PLB25_17025"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOE43312.1"
                     /translation="MPETKPSRRGFLKASGAVLSVSSLMGTAALMAKSDDVKATVEWA
                     EHFQGNYRLMTDAEKAEARARLEKRYSAQYHKKVIVDGTEAAPGVLMGYALNIKKCIG
                     CRRCVKACVEENNQSRGEKPGERIEWIQVLRMERGEFSNDKMNQGYPEGLGIQVGGNA
                     YTPAGAVLEGQYHYEPEAVPEKDANYMPIACMQCEKPPCVKVCPVRTTYREADGPVVI
                     DYNWCIGCRMCMSACPYWARRMNVTTPVLPKEDMNPVTHYLGNRPRMRGVVEKCHWCL
                     QRTRQGRYPACVEVCPVGARKFGNLLDPESEVSKILARKNVFRLKAELNTFPKFFYFY
                     D"
     gene            3378..>4035
                     /gene="nrfD"
                     /locus_tag="PLB25_17030"
     CDS             3378..>4035
                     /gene="nrfD"
                     /locus_tag="PLB25_17030"
                     /inference="COORDINATES: protein motif:HMM:NF015851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HOE43313.1"
                     /translation="MNTLISFVPDYWRYVLKGGAKFYSWMGLLAVLSCIAMYGFYLQN
                     TEGLIVTGLTNQIHDGLYFANLVFLVGVAAGAVTIVFPAYVYHNEAMHKVSVLGEMLA
                     ISAVVMVMLFVFAHMGRPDRLWHMIPYIGIYNLPGSMLGWDVLVLNGYLMLNFACGFY
                     YLFTKYSGSEVNKKIFMPLIYISIVWALSIHTVTAYLIATMPARPMWHYSIIPIRFIV
                     T"
BASE COUNT          905 a         1131 c         1105 g          894 t
ORIGIN      
        1 aagtgggatt acttcgtggt ggagaagtcc cccgtgctaa ctgccctcat tgaacgcagg
       61 ggcaacgtgg acagcgtgat gctgctcacc accaagaccg gtcgggccgt gtcggcgtcc
      121 atgctgcgcg cacggtacga cgacgcccgc atcaaggccg ccgccgcgaa ccccaaactg
      181 gccgacgaca tcaaggccat gttcctgcgc gatacccgca agcgcgctgc cgacttggcc
      241 gacgatgtag acgaggcgtc gaaactgttg cagcacagca gtaaagccct gactgcgaag
      301 cattaccgga caaaaggaac agagctcaaa gcagtgcgat aaatcgctcc cgcactacca
      361 caccctgaga gatagaaagg tgaatctaag tgtggaatat atgcggaagt aatcggcctg
      421 caggccgcac ccagcttaga atcgcccatg ggctcacccc ccgccgcaag gcgtgccggt
      481 tcgattccgg ccccaggcac caattaaaca gtgattttgt tgctattgaa tttgtagcaa
      541 atcacaatca cttccgcatg ctgttcgctt gacagcgcca ggcggtccaa ttctccatca
      601 ggcgattagt gggacttgaa tccagcgaca aactgacgtt tgctgatcgc gaaccgttaa
      661 cgcgcaactg catgctgcag aacaaagtcg gcgattgagc tcgtgtccgc cagatccagg
      721 atgggtagtc ccttgccgtc aagaggcgta tcgcaggcca gcactgcaat gcccggccag
      781 tcgggccaca gcgtttcctt actttgacca tcgcgccaaa cttccagctt gggaaaaccg
      841 ccactcttga aaccttcaat aaggaccagg tcacagttct gcatacggtc cagtaagtag
      901 tccagtgagg gctcttgcgc gccacgcatt tcatgcatca gtgcccaacg gtcactgcca
      961 agaagcagaa cctccatgca gcctgattcc cgcaggcggt aagagtcctt tcccgggcgg
     1021 tcaatatcga tatttttgtg actgtgcttt atgagtgaca ccaccaaacc tcttgccgcg
     1081 atctctggca ccagcttttc cagcagcgtg gtcttaccca tgccggagtg accagcaatt
     1141 ccaaaaatcc tcatgcaaac gtcctggtta gttgttcaat ttgcaacgcc aagtctcgtg
     1201 ccttgtcgtg gtcggtcgca aattgacgtg aacaacgtaa tgtcgattct tcgctgataa
     1261 gtgtagatca cttcgtcgag tagaagcccc gtgcgtggac attgctggct agttgcaccg
     1321 gcgcacgaac atcggcagca agccatcgcc tccgccaagt attgcgctgt acttgttcca
     1381 gtaaccagca ggggttgttc agagcgaaag ccgcgatgca atgggcggtc ttacgataga
     1441 ttctgtgaat tggctcagag ctctttcagc tctgacagtc tgacaggcgg catgtcggtt
     1501 tgacctgact ttctgacatg gccgatgccc gcagatgcat agatgcaata aatatttctt
     1561 agaaatcaac gggttacaga aggtcttttg ctggcatgcg cattgcaagc agatgctgct
     1621 aggcaacgag gcatggagat gccgctgcgc ccgttgctga ttcaaactct ttggaaacac
     1681 aatgaaattc gtacacatcc tcatcggcgc atctgttttg acttgtgccc cagccttcgc
     1741 cagcagtgca ttggcgactc aaaaagcttg cttggcgtgc cacactgctg acaaaaaatt
     1801 ggttggtccc tcctaccagg aagtcgccaa gaagtacgcc ggtcagaaag atgcgctggc
     1861 cgttctgacc aaggcgatca agacgggtgg aagtggaaaa tacggtcccg ttcccatgcc
     1921 gccacagacc gcattgagcg acgccgacgc caagacactg gcggcttggg tgctcgctgg
     1981 cgccaagtag ccgcctccaa ttctggcttg acggcctagg gttgaatcgc gctctatggc
     2041 cgagaggtcg tgctaccgct cacagattcc agatggttag cgctgcgatc acaaacccgg
     2101 tttctgcctc acttgttttc ggttgatgcg atgtttctag cctgttaagc attgcaggaa
     2161 tagaccacgc ctctgccttg gtcggttggg tgttgcctga tgggtttcat catgcgcggc
     2221 cattgctctg gaatttcgcg ctaaaggcgt cttttttgta gtggagaatg aatgaaccat
     2281 aacgaagccc cccctagcgc cccttgtgga gcgaaatcat gccaatgcgc gactccagcg
     2341 gcgtcggcaa atcctgtgtt gcctgagacc aaaccgtcgc gccgtggctt cctgaaggcc
     2401 agtggcgctg tgctaagtgt gtcttcgctc atgggcacag cagcgttgat ggccaagtct
     2461 gacgatgtga aggccaccgt cgaatgggca gagcatttcc agggcaacta ccgcctgatg
     2521 accgacgcgg aaaaggccga agcgcgcgca cggcttgaaa aacgctactc ggcccagtac
     2581 cacaagaagg tcattgtgga tggtaccgag gccgcacccg gcgtcctcat gggttatgca
     2641 ctgaatatca aaaagtgcat tggatgccgc cgttgcgtca aagcctgcgt ggaagaaaac
     2701 aaccagtcgc gtggcgagaa acccggagaa cgtatcgagt ggatacaggt gctgcgcatg
     2761 gagcgcggtg agttctccaa cgacaaaatg aaccagggct acccagaagg tcttggcatt
     2821 caggtcggtg gcaacgccta tacacccgcc ggtgccgtgc tggaaggaca gtaccactac
     2881 gaacccgaag ccgtccctga aaaagacgcg aactacatgc caatcgcctg catgcagtgt
     2941 gaaaagccac cctgcgtcaa ggtgtgcccg gtgcgcacca cctaccgtga ggcggatggc
     3001 ccggtggtga tcgactacaa ctggtgcatt ggttgccgca tgtgcatgag cgcctgcccc
     3061 tactgggcgc ggcggatgaa cgtcactacg ccggtgttgc caaaggaaga catgaacccg
     3121 gtaacccatt acctgggtaa ccggccccgc atgcgcggtg tggtcgaaaa atgccactgg
     3181 tgcctgcaac gcacccgtca gggtcgctat ccggcttgtg ttgaagtgtg cccggtaggt
     3241 gcgcgcaagt tcggcaatct gctcgatccg gaaagtgaag tgagcaagat tttggcccgc
     3301 aagaacgtgt tccgcctgaa agccgagttg aacacgttcc cgaaattttt ctacttctac
     3361 gattgaaggt gcaaatcgtg aacacgttaa tcagtttcgt gcccgactac tggcgctatg
     3421 tcctcaaggg tggcgccaag ttctacagct ggatggggct tttagccgta ttgagttgta
     3481 tcgccatgta cggtttctat ctgcaaaaca ctgaaggctt gatcgtgacc ggccttacca
     3541 atcagattca cgatggcctg tactttgcca acctcgtgtt tctggtcggc gttgcagctg
     3601 gtgccgtcac catcgtcttt ccggcctacg tctaccacaa cgaggcgatg cacaaagtct
     3661 cggtgctggg tgaaatgttg gccatttctg cggtggtgat ggtcatgttg tttgtgtttg
     3721 cccacatggg caggcccgac cggttgtggc acatgattcc gtacattggc atctacaacc
     3781 tgccgggatc catgctgggg tgggacgtgc tggtgctgaa cggctacttg atgctcaact
     3841 ttgcctgcgg cttctattac cttttcacca agtattcagg ctccgaagtc aacaagaaaa
     3901 tcttcatgcc cctgatctat atctccatcg tctgggcact gagcatccac accgtgacgg
     3961 cgtaccttat tgcgaccatg ccggcacgcc cgatgtggca ttacagcata ataccaatcc
     4021 gcttcatcgt gaccg
//
LOCUS       DAOSLL010000462         4030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19669_length_4030_cov_5.225912, whole genome shotgun sequence.
ACCESSION   DAOSLL010000462 DAOSLL010000000
VERSION     DAOSLL010000462.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19669_length_4030_cov_5.225912"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1975)
                     /locus_tag="PLB25_17035"
     CDS             complement(14..1975)
                     /locus_tag="PLB25_17035"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOE43314.1"
                     /translation="MSDADQDFIAAAMPAFISEAGEQTQAIEALLLELEVQPDNRELL
                     DTLFRCAHTVKGSAGIFGLTKVVEFTHHVETLLDKLRDGDLSLTPVLSTLLLQCNDQI
                     KFLVDTASDDSADTADQKDLRADLIIQLRACTEGTAAPQAGAGKGGLQTPNDPHAANM
                     PGITDWKISARFGPETFRNGMDPLSIARYLKGMGVVTSMVCGTDSVPTLVNLNPETCY
                     LSFGMELTSTASREEVEGAFSFVADDCELDVIAPETKGQKLVRAIEDMAATPRLGDML
                     VSVGAVSQEKLTEVLSNQGQTGGSAKADKPKLGNLLETQAGVAPEVVEAALSKQQKAR
                     EAGGGEENRYIRVQADRLDLVINLLGELVIAGAGANLLARETRNVALIEANLHMNGLI
                     EEIRNGTLGLRMVPVGETFSRFRRVVRDTASSLGKEVNFEIVGGEAELDKSMVEKIAD
                     PLMHLVRNSLDHGLEPPQERVAAGKAPAGKLTLSARHETGAILIRIEDDGRGINRERV
                     LQRAWNRGLIEPGTVPSDDVINMMIFEPGFSTAEQVTNLSGRGVGMDVVRRNIEALRG
                     SLKLNSQPGRGLQVDIRLPLTLAIIDGFLVGVGKAKFVLPLGSVVEVIEAGDQHVRVD
                     ATGRHCVELRGAVLPVVRLRSLYNIESTL"
     gene            complement(1990..2277)
                     /locus_tag="PLB25_17040"
     CDS             complement(1990..2277)
                     /locus_tag="PLB25_17040"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOE43315.1"
                     /translation="MSTPFELPQEMTIYSAVETRDALLGWVTEQNAKSARLLEISAAK
                     VEEIDGAGLQLLAALANTEQNWQLVNPSGIFVDACKALGLIAWLDKSPVIA"
     gene            complement(2356..2724)
                     /locus_tag="PLB25_17045"
     CDS             complement(2356..2724)
                     /locus_tag="PLB25_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE43316.1"
                     /translation="MAQTILVIDDSASLRQVVAMALQGSGYEVLQAGDGQAALAMLDG
                     RKIHMAICDVNMPRMNGIEFVKAAKALPNYRFLPILMLTTESQEAKKQEGKAAGAKAW
                     MVKPFSPASLLNAVSKLCLP"
     gene            complement(2737..3378)
                     /locus_tag="PLB25_17050"
     CDS             complement(2737..3378)
                     /locus_tag="PLB25_17050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43317.1"
                     /translation="MSAPAHSDSSADHAQALVALCSQVLPIWARQLASSRSQSEGAVA
                     DMLAAFAEIGPHLDLATRQSEQIAAALAKSSSGITGLADACKQELKPLLPGLDAPTAA
                     ALGRVLSMIRDSVDALERISKPFERETQMVGQQVERMYKGFQYQDRISQMMTLLHEDI
                     ERLKMAIDDETADVDAVAWLARLESQYVMTEQRHHHQHDAGTAAVDDDETTFF"
     gene            complement(3375..>4030)
                     /locus_tag="PLB25_17055"
     CDS             complement(3375..>4030)
                     /locus_tag="PLB25_17055"
                     /inference="COORDINATES: protein motif:HMM:NF028509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOE43318.1"
                     /translation="QINETMESLISGIGEIAMNVNVMAEGSATQSTALEEVVHAVGDL
                     DVLTHENAELVERASFNSDRMIAQASTLEISVGDIRLRQGTADQARQMVFDAMVHIRT
                     HNLEQAIADFHDKQGPFIDRDLYVFIFDRSGHYVVHGAQPEKDGSSLSSIAGLDADKL
                     VADAWAACDHEQGGWVNYTITNPVSGEVQGKSSYVMPLDEDRLIGCGCYLNAQWLNL"
BASE COUNT          825 a         1217 c         1122 g          866 t
ORIGIN      
        1 gattgcgttc gctctacaac gtcgattcaa tgttgtagag cgaacgcaat cgcaccaccg
       61 gcagcaccgc accgcgcagc tcaacgcaat ggcgaccggt ggcatcgacc cgcacatgct
      121 gatcaccggc ttctatgacc tcgaccaccg agcccagcgg tagcacgaac ttggccttgc
      181 ccacccccac cagaaaaccg tcgatgatgg ccagcgtcag tggcagccga atatcgacct
      241 gcagcccacg gccgggttgg ctgttgagct tgagtgaccc acgcagcgct tcaatgttgc
      301 ggcgcaccac atccatgccg acaccacgac cagacaggtt ggtgacctgc tcggcagtcg
      361 agaagccagg ctcaaaaatc atcatgttga tgacatcgtc gctcggcact gtgccgggtt
      421 caatcagccc gcggttccag gcgcgctgca acacccgctc gcggttgatg ccacggccat
      481 cgtcctcaat gcggatcagg atggcaccgg tttcgtgacg cgcgctcagg gtgagtttgc
      541 cagccggagc tttgcctgcc gccacgcgtt cttgcggcgg ttccagcccg tggtcgagcg
      601 agttgcgcac cagatgcatg agcgggtcgg caattttttc gaccatcgat ttgtcgagct
      661 cggcttcacc gccgacgatc tcgaaattga cctctttgcc cagacttgac gcagtgtcac
      721 gcaccacgcg cctaaagcgg ctgaaggttt cccccacagg caccatgcgc aggcccagcg
      781 tgccattgcg gatttcttca atcaggccgt tcatgtgcag attggcctca atcagcgcaa
      841 cattgcgggt ttcgcgcgcc aacaggtttg ccccggcccc ggcgatcacc agttcgccca
      901 gcaggttgat gaccagatca aggcggtcgg cctgtacccg gatatagcga ttttcctcgc
      961 caccaccggc ttcgcgggcc ttttgctgct tgctgagcgc cgcctcaacg acttcgggag
     1021 cgacccctgc ctgggtttcc agaagattgc ccaatttggg cttgtccgct ttggccgagc
     1081 caccggtttg cccctgattc gagagcactt cggtgagctt ttcctggctg acagcaccta
     1141 ccgacaccag catgtcgccc agacgcggcg tagctgccat gtcttcaatg gcgcgcacca
     1201 gcttttgccc cttggtctct ggcgcaatca catcaagttc gcaatcgtca gcaacaaaac
     1261 tgaacgctcc ctcaacttcc tcacgcgagg cagtcgatgt cagctccata ccgaaactca
     1321 ggtaacaggt ctcggggttc aggttgacca gcgttggcac cgagtcagtg ccgcacacca
     1381 tggatgtgac gacccccata cccttgagat agcgggcaat tgacagaggg tccatgccgt
     1441 tgcgaaaagt ctccgggcca aatcgggctg atattttcca gtctgtgatg ccaggcatgt
     1501 ttgccgcgtg tgggtcattg ggcgtttgca acccgccttt gccagcgccc gcctgcggcg
     1561 cagcggtgcc ctcggtgcaa gcgcgcaact gaatgatcaa atctgctcgc aggtcttttt
     1621 gatcggcggt gtcagcgcta tcgtcgctgg cggtatcgac caaaaacttg atctggtcat
     1681 tgcattgcag cagcaaggta ctgagcaccg gggtcaggct gaggtcaccg tcgcgcagtt
     1741 tatcaagcag cgtctcgaca tggtgggtga attccaccac cttggtcaaa ccaaaaatgc
     1801 ccgctgatcc cttgacggtg tgagcgcagc gaaacagggt gtcgagcagc tcacggttgt
     1861 caggctgcac ttcaagctca agcaacaggg cttcaatggc ttgcgtttgc tcgccggcct
     1921 cgctgatgaa ggcgggcatg gcagcagcaa tgaaatcttg gtcggcatct gacacggcaa
     1981 tgctcccttc taggcgatga caggcgattt gtccagccag gcgatcaagc ccaaggcctt
     2041 gcaggcatcg acaaagatac cgcttgggtt gaccaactgc cagttttgct cggtgttggc
     2101 caatgcagcc aacagttgca ggccagcgcc atcgatttct tcaaccttgg ccgcagagat
     2161 ttcgagcaac ctggcagact tcgcgttttg ctcggtaacc caacccagca gggcatcacg
     2221 ggtttcgact gcgctgtaga tggtcatttc ttgcggaagc tcaaagggtg tgctcataaa
     2281 aatcctgaaa tagtgcaaaa aacggggaac tgcgacacca ccagcatcag gtgtcactgc
     2341 attctcaagc ttccgttatg gcaggcacag tttcgatacg gcattgagca atgaggcagg
     2401 tgaaaagggc ttgaccatcc aggctttggc accggccgcc ttgccctctt gctttttggc
     2461 ctcctggctt tcggtggtga gcatcaggat tggcaaaaac cggtagttgg gcaaggcctt
     2521 ggccgccttg acaaactcga tgccgttcat gcgcggcatg ttgacatcgc aaatcgccat
     2581 gtggatcttg cggccgtcca gcatggcaag tgcggcctgt ccgtcgccag cttgcagaac
     2641 ttcatagcca gatccttgca acgccatggc taccacctgg cgcaggctgg ccgagtcgtc
     2701 aatcacgaga atggtttgtg ccatcgaaaa tccccatcaa aaaaaagtgg tttcgtcgtc
     2761 atcaaccgca gccgtgccag cgtcatgctg gtgatgatgg cgttgttcag tcatgacgta
     2821 ttgcgattca agccgcgcca gccaggcgac tgcatcgaca tcggcggtct cgtcgtcgat
     2881 ggccattttc aggcgttcaa tgtcctcatg cagcagggtc atcatctggc tgatgcggtc
     2941 ctggtactgg aagcccttgt acatgcgctc aacctgctgg ccaaccattt gggtctcacg
     3001 ctcaaaaggc ttggagatac gttcaagcgc gtccaccgag tcccgaatca tgctgagcac
     3061 ccgccccaat gccgcagcag tcggtgcgtc cagaccgggc agcagcggct tgagttcttg
     3121 cttgcaggca tcggccaatc cggtaatgcc gctgctgctc ttcgccaatg ccgcagcaat
     3181 ttgctcggac tggcgtgtcg ccaagtcaag atgcggacca atttcggcaa aagcagccag
     3241 catgtcggcc acggcaccct cggactgact gcgggaactg gccaattgac gggcccagat
     3301 cggcaacacc tgactgcaca aggccaccag cgcctgtgcg tgatcagccg acgagtctga
     3361 atgggctggc gcgctcataa attgagccac tgcgcgttca ggtagcaacc acagccaatc
     3421 agacgatctt cgtcgagcgg catcacgtac gaagatttgc cctgcacttc acctgaaacc
     3481 gggttggtga tggtgtagtt gacccagcca ccctgctcgt ggtcgcacgc ggcccaggca
     3541 tcggcgacca gtttgtctgc atcgagcccg gcaatggaac ttaatgagct accgtctttt
     3601 tcaggctggg caccatgcac cacataatgg ccgctccgat caaaaatgaa aacatagaga
     3661 tcgcggtcaa tgaatgggcc ctgcttgtca tgaaaatcag caatggcctg ctcaaggtta
     3721 tgggtgcgga tgtggaccat ggcatcaaac accatttgcc gggcctggtc agccgtgccc
     3781 tggcgcaagc ggatgtctcc cactgatatt tccagggtcg aagcctgggc gatcatgcgg
     3841 tctgaattga agctcgcgcg ctccaccagt tcggcatttt catgtgtcag cacgtccagg
     3901 tcaccgacgg catgcaccac ctcttcaagg gcggtacttt gcgtggcgct gccttctgcc
     3961 atgacgttga cgttcatggc aatctcgcca atgcccgaaa taaggctttc catggtttcg
     4021 ttgatttgct
//
LOCUS       DAOSLL010000463         4030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19671_length_4030_cov_4.417610, whole genome shotgun sequence.
ACCESSION   DAOSLL010000463 DAOSLL010000000
VERSION     DAOSLL010000463.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19671_length_4030_cov_4.417610"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLB25_17060"
     CDS             <1..297
                     /locus_tag="PLB25_17060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43319.1"
                     /translation="AFSGSQFLKPPALPGDGYKLYDLHFHVPEVGYIQAAALGFQCIW
                     LTWCAVTKMCLTIECLDQWCFLTDGSNASRANKHGAQARTNQAGIIGIRHSAFK"
     gene            complement(514..3273)
                     /locus_tag="PLB25_17065"
     CDS             complement(514..3273)
                     /locus_tag="PLB25_17065"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE43320.1"
                     /translation="MLLLSWLNPAWLGRLDLLAYDLLLPNPPVWLESPLIIAIDDASL
                     QELGRWPWPRARHADMLERLDKAGASAIGLAVLFGEPDASDPAGDAALAAAVTRSRRV
                     VLPVAPEPLASGRMASAPAGWLLGQTKAPLGHVDIEIDMDGQARRLYLQAGYARADVP
                     ALALAVRQIADPNWRTTDMPGVRANPPKASPVKQWMRDYEVLLPRLQGLPTLSFSSVL
                     HQPDQLALVRDRAVFIGVTATGLGGELVTPLGGARATVPAVVLHGHAYAALAQHTLMQ
                     RAPAWLSLLVSLVLLTALALWPRPQGRQSLWLLSHLGLPVLVSLMALHWLGLWLGPAQ
                     GVLILASALLVMAAAGLQRTRQQLHRSRQHLAATLQAIGDAVITLDARSGTIRYANPT
                     ALAQADRDQLIGLSLRQAYPLSIDSHDRIELAVAESMQQLRRVNLPTNLTLQPRQHSD
                     TRFLSGSASPLIGPQGQLDGTVLVLSDQTAAVTAARQLDYAATHDELTGLPNRAMLHQ
                     RLGLALTRSQRHGGNTAILFLDLNRFKVINDSLGHRAGDEVLKVVAKRLRQTCRETDL
                     VARWGGDEFVLVLEDMIDHEGAARAAAKVIEVLTQEVELSGTFGNLRLPSTGSVGVVV
                     APQDGVDMDDLLAKADMAMYRAKAQPQACFQFWSEDLNTSLRASLALELDLRKGLNEH
                     QLVLYYQPQFSLAGRELVGMEALMRWQRTPESLLMPGEFIPVAESSGLIIEMGAWAVL
                     QAARQLAAWLAVGLKPAPIAVNVSARQCLNRDIVDVVRLALQETRIPPALLRLEITES
                     IAMSNVDQVIELLQAIRALGVGLALDDFGTGFSSLSYIKRFPIDEIKIDRSFVIDIAS
                     NREDLAIVRATIALAHGLNMKVVAEGIETEAQGQILISERCDVGQGYLYGFPQPINQV
                     TRLVR"
     gene            complement(3384..>4030)
                     /locus_tag="PLB25_17070"
     CDS             complement(3384..>4030)
                     /locus_tag="PLB25_17070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HOE43321.1"
                     /translation="EGHSSQRALTVHARPEPPLLIAPAASAVTDLAQPAFRWTQAQPH
                     WRYRLQIIDIASDTSLDEQVVDSDVSPLSRRELPAGQYSWRVAAIDPVRGQGPWSDWQ
                     MFRRVLPGPGVTPTEAVDGGLSLRWSAQPQVAHYHLQVGPDEAFAMPLVDAQPDLPQY
                     QLSGLTAGEYRVRVRAVGTDGYVGPWGSTQRFVVPEAPRPWWSLVLFLAVIGLL"
BASE COUNT          840 a         1264 c         1185 g          741 t
ORIGIN      
        1 gcttttagcg gctcacaatt cttaaagccc ccggctttgc cgggggatgg ttacaagctg
       61 tatgacctcc attttcatgt tcccgaagtt ggttacatcc aagcagcagc gttaggtttc
      121 cagtgcattt ggctgacgtg gtgcgcagtg acgaaaatgt gtttgactat cgagtgtctt
      181 gaccagtggt gtttcctcac ggacggcagc aatgccagcc gcgctaacaa acacggcgcg
      241 caggctcgca cgaatcaggc aggaatcatc ggcattcggc attcagcgtt caaatgagta
      301 ttcacgacga tacctgcccc gacccgcatt tcactatcgg gcaatgctga tctggctgta
      361 ggacgatgct gagcggggag tcagaattgc ccgacccgtc gctgctgtcg tccggccacc
      421 agcaggcttt cggtcagcac cagcttcaaa ttcacacggg tttcttccgc cagcgcaata
      481 ggggcgggcc acaacgcggt aaggaatcca ggttcaccga accagccgcg tgacttggtt
      541 gatcggctgc ggaaacccat acagatagcc ttgtccaaca tcacaacgct cggaaatcag
      601 aatctggccc tgtgcttcgg tttcaatgcc ttctgccacg actttcatgt tcagtccatg
      661 cgccaaggca atggtggcgc gaactatcgc caggtcttcc cggttgctgg caatgtcgat
      721 gacaaaggag cggtcaattt ttatctcatc aataggaaac cgtttgatgt agcttagcga
      781 agaaaagccg gtgccaaagt cgtccaatgc caagccgaca cccagcgcgc gaatcgcttg
      841 caacaactcg atgacctggt ccacattgga catggcgatg gattcggtaa tctcgagccg
      901 tagcaaagct ggcggaatac gggtttcttg cagggccagc cgaacgacat cgacaatgtc
      961 ccggttgaga cattgacgtg ccgacacgtt caccgcaatc ggcgcgggct ttagcccgac
     1021 tgccagccag gctgccaact ggcgggccgc ctgcagcact gcccaggcac ccatttcgat
     1081 gatcaagccc gaactctcgg ccaccgggat gaactcgccc ggcatcagca gactctcggg
     1141 cgtgcgttgc catcgcatca gcgcctccat cccgaccaac tcgcgacccg ccaacgaaaa
     1201 ctgcggctgg taatacaaca ccaactggtg ttcgttcagg cccttgcgca aatcaagttc
     1261 gagtgccaaa gacgcgcgca ggctggtgtt gaggtcttcc gaccaaaatt gaaagcaggc
     1321 ctgaggctgc gccttggccc ggtacattgc catgtcggcc ttggccagca gatcgtccat
     1381 gtcaacgccg tcttgcggcg ctaccaccac gcccacgctg ccggtactgg gcagacgcag
     1441 gtttccaaaa gtaccgctga gttcaacctc ctgggtcaac acttcgatca ctttggcagc
     1501 ggcccgggca gcaccttcgt gatcaatcat gtcttcgagt accagcacaa attcatcacc
     1561 accccaacgt gccaccaagt cggtttcgcg acaggtctgg cgcaggcgct ttgccaccac
     1621 cttcaacacc tcgtctccag cacgatgacc caaactgtca ttgatgacct tgaaccgatt
     1681 caagtccaga aacaatatcg cggtgttgcc gccgtggcgt tgcgaccgtg tcagcgccaa
     1741 tcccagccgc tggtgcagca tggcccggtt gggaagaccg gtcagctcgt catgggtggc
     1801 cgcatagtca agctgacgcg ctgcagtaac cgccgcagtt tgatcagaca ataccagcac
     1861 ggtgccgtca agctgaccct gcggcccgat caacggactt gccgaaccgc tcaaaaagcg
     1921 ggtgtccgaa tgctgccggg gctgtagcgt caagttggtc gggaggttga cgcgacgcaa
     1981 ttgctgcatg gattcagcca ctgccagctc gatccggtcg tggctgtcga tcgacaaggg
     2041 atacgcctga cgcagtgaca atccgatcag ttggtctctg tcagcttgcg ccaacgccgt
     2101 cggattggcg tagcgaatgg tgcctgagcg ggcatcgagg gtgatcacgg catcaccaat
     2161 cgcttgcaag gtggccgcca ggtgctgacg cgaccgatgc aattgctggc gtgtgcgttg
     2221 tagcccggcc gctgccatga ccagcaatgc gctagccaga atcagcacgc cttgcgctgg
     2281 acccagccac agtccgagcc agtgcaacgc catcaggctg accagcaccg gcaagccaag
     2341 atgactcagc aaccacaagg attggcgccc ttgcgggcgc ggccacagcg ccagcgcggt
     2401 gagcaacacc agtgacacca gcaaggacag ccatgcaggt gcccgctgca tcagggtgtg
     2461 ctgcgccagg gccgcatagg cgtggccatg caacacgact gctggcactg ttgcgcgcgc
     2521 accgcccagc ggtgtcacca gttcgccgcc caggccggtg gcggtgacac caataaagac
     2581 cgcccgatca cgaaccagcg cgagttgatc tggctggtgc agcacgctgg aaaaagacag
     2641 ggttggcaga ccttgcaggc ggggtaacaa gacttcgtag tcgcgcatcc actgtttgac
     2701 agggctggcc ttgggtggat tggcgcgcac accgggcatg tcggtggtgc gccagttcgg
     2761 gtcggcgatt tgacgcacgg ccagcgccag cgcaggcaca tctgcccgag cgtaaccggc
     2821 ttgcaggtaa agtcggcggg cttgcccatc catatcgatt tcaatatcga catgcccaag
     2881 cggggccttt gtctggccga gcaaccagcc ggctggcgca gatgccatgc gtccggatgc
     2941 cagcggctcg ggcgccactg gcagcacgac ccgtctgctg cgcgtgaccg ccgctgccag
     3001 ggcggcatcg cctgccgggt cgctggcatc gggctcgcca aacagcaccg ccaagccgat
     3061 ggctgatgca cccgccttgt caaggcgctc cagcatgtcg gcatgacgtg cgcgtggcca
     3121 cggccaacgt ccgagttctt gcaggctggc atcgtcgatg gcgatgatca acggcgattc
     3181 aagccacacc ggtggattgg gcagcagcag gtcgtaagcc aaaagatcaa gccgaccaag
     3241 ccaggccggg ttcagccagg acagcagcag caaggtggca actcctgtta gccaccacgc
     3301 ccagcgcaat acccatcggg gcagatggtg tagcccccgc ccatcttctc gcaggactgt
     3361 gagcagtgcc tgccgagcca gcatcagagc aagccgatga ctgccagaaa cagcaccaaa
     3421 gaccaccatg gtcgcggtgc ctccggtacg acaaatcgct gggtactgcc ccaaggcccg
     3481 acatagccgt cggtgccgac tgcccgaacc cggacccggt actcaccagc ggtcagaccc
     3541 gacaactggt actgtggaag atcgggctgt gcatcgacca gtggcatcgc aaaggcttcg
     3601 tcaggcccga cttgcaggtg gtaatgagcg acttgtggct gggcactcca gcgcaggctg
     3661 agtccgccat caactgcctc ggtgggtgtc acgcccggac ctggcaaaac ccgccgaaac
     3721 atctgccagt cactccatgg gccttggccc ctgaccggat caattgccgc cactcgccaa
     3781 ctgtattgcc cggctggcaa ttcccgccgg gatagcgggc tgacatcgct gtctaccact
     3841 tgctcgtcca ggctggtgtc actggcgatg tcgatgatct gcagtcgata gcgccaatgc
     3901 ggctgtgcct gggtccagcg aaacgctggt tgtgccaagt cagtgaccgc tgatgcagcg
     3961 ggggcgatca gcagcggcgg ctccggccgg gcatgcacgg tcaaggcacg ttgcgaagaa
     4021 tgcccctcca
//
LOCUS       DAOSLL010000464         4030 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19673_length_4030_cov_4.109182, whole genome shotgun sequence.
ACCESSION   DAOSLL010000464 DAOSLL010000000
VERSION     DAOSLL010000464.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4030)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4030
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19673_length_4030_cov_4.109182"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(4..666)
                     /locus_tag="PLB25_17075"
     CDS             complement(4..666)
                     /locus_tag="PLB25_17075"
                     /inference="COORDINATES: protein motif:HMM:NF014651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD family protein"
                     /protein_id="HOE43322.1"
                     /translation="MSDYQPDESIARVDLLRFLSACYYEPTADFTEEKLFDSIIAATR
                     QAYPELAGLAVRLGQSFADDRLQTLLVDYTRLFLGPMKALASPYGASWLPAPMVGDDV
                     LPPLILDLYGEGGFEVDAELAELPDHVAIELEFLYLLAFNQRQADLHGNFADFKSIAN
                     LRKRFLDEHLSAWISPFTNAVKNSAETAFYRVLAELTEGVIRLEADRHSGADVALVDP
                     PV"
     gene            complement(707..1522)
                     /locus_tag="PLB25_17080"
     CDS             complement(707..1522)
                     /locus_tag="PLB25_17080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOE43323.1"
                     /translation="MTQFGMVIDLAKCNGCGACAFACKAENNTRDRGDLQSFNWADFL
                     MHTAGKFPNTTQSVIPVLCNHCSDAPCVKICPGNPEKAGNPTKSPDGKPYKALYKTPE
                     GITMHDPELCVGCGDCQKECPYSHPRLDASSLTGASYSVISLNTSGENPQPRWSDKTS
                     AIPGCTSSGAEVAKLAGAIPPMGNLWKSGDQQAVRADDVIEKCTFCYHRVLNGLQPAC
                     VEACPAKARIFGDQKDPNSEIAKILKAEKSWRLQEEKGTRPNVHYIGKYSPRA"
     gene            complement(1556..>4030)
                     /locus_tag="PLB25_17085"
     CDS             complement(1556..>4030)
                     /locus_tag="PLB25_17085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HOE43324.1"
                     /translation="PGPVHSSLIPRKAKPCSPAAGNWVASTCQGCTQWCAVQIFVQDG
                     RATRVRGNPLSKSNHGYCCPRGHLIPQQMYDPDRIKTPMKRTNPLKGRGIDPKFVPIS
                     WDEALDTVADKMMELRKAGEPGKLMYMRGRYSSTSTELLYGTLPKVWGTGQYFSHSAL
                     CAEAEKMGPGLTQGFFGYRDYDLGNTNCLVVWGCDPLSSNRMVPNTMHHFPGIVKRGT
                     VIAVDPRMSTIAAKAHEWLPINPGTDGALASAIAHVILTEGQWGKEFVGDFKDGKNLF
                     VTGKLVDEAAFAEKESNGVVKWWNLELKDKTPAWAEKETLIPADQILRVARAMGKAGS
                     KTTVWMGPGVAMTPRGTYAAMAVHALNGLLGSIDTEGGVWQSSSVPLAKFPSMDKYVD
                     DVAKAGSKGKKLDGRGAKDMPAMMNAVPGSGVVTNNVANGLLKDPGAVKVFLSSWSNF
                     NFSATGAGRWDKAMAKIPFFVHMVPNASEMTMFADIVLPSTFNSAEGWSIVSNMGNGH
                     GYASIQQGAVKRLWDVKQEETEVMWLLAEKLKAKGFPNLFDYYSKEFKDPETGKLPTT
                     AMEFSEIAAKISSATLWMPKEPLKGDAPIKGWAEFKAKGMFSGPKYTLKKGWGGKFGT
                     VTKKFEFYSETLKKGLLEHAKKYETTTDDIMTVSGYVARGDLAFVPHYEPPKRHGSVA
                     EYPFTFIDHKSRLNREGRSANVTWYQEFKKVDPGDVSWGDVVKMNPSDGARLGLKDGE
                     NVKITSVAGTIACQLKLWEGVRPGTVAKCYGQGHWAYGRVAAKDFVKAIAFGGNNNEV
                     LVDDYDRLSGATARNGGFTGVRIEKA"
BASE COUNT          798 a         1152 c         1152 g          928 t
ORIGIN      
        1 gcgctagaca ggcgggtcaa cgagggccac gtctgcccca ctgtgccggt cggcttccag
       61 ccgaatgaca ccttcggtca actccgccag aacccggtaa aaagcagttt cggcactatt
      121 cttgaccgcg ttcgtgaacg gcgagatcca ggcactcaga tgctcatcca agaagcgttt
      181 acgaagattg gcgatggatt tgaagtcggc aaagttgcca tgcaagtccg cctgtcgctg
      241 gttaaatgcg agcaggtaca agaattccag ttcaatcgct acatgatcag ggagttcagc
      301 caattcagca tcgacttcaa agccgccctc accataaaga tcaagaatca acggcggcag
      361 tacatcgtca ccgaccattg gtgctggcaa ccatgaagca ccataggggc tggccagggc
      421 cttcatgggt cccaaaaaca gccgtgtgta atccaccagc agcgtttgca ggcgatcatc
      481 ggcaaacgac tgacccagtc ggactgccaa gccagctagt tccgggtagg cctgtcgcgt
      541 tgccgcgatg atcgagtcaa acagcttttc ctcggtgaaa tcggctgttg gctcgtagta
      601 acacgcggac agaaatcgca acaggtccac ccgtgcgatg gactcgtcag gctgatagtc
      661 ggacatacgt gacgatgaag ttacaggggc ttggccagat cgacgatcag gctctagggc
      721 tatatttgcc aatgtaatgc acattgggtc tggtaccctt ttcttcctgc agacgccagg
      781 acttttcagc cttcagtatt ttggcgattt cgctgttggg gtctttctga tccccaaaaa
      841 tacgcgcttt ggctgggcag gcttccacgc aggcgggttg caagccgttg agcacgcggt
      901 ggtagcagaa ggtgcatttt tcaatgacat catccgcgcg cacagcctgc tggtcgccac
      961 tcttccacaa gttgcccatc ggcgggatcg ctccagccaa cttggccact tctgcacctg
     1021 aagaggtgca tcctggaatc gcagaggtct tgtcggacca gcgcggctgc gggttttcgc
     1081 cggatgtgtt gagactgatg acgctgtagc tcgcacctgt caggctgctg gcatccagcc
     1141 tggggtggct gtacgggcac tccttctggc agtcgccaca accaacacac aactcagggt
     1201 cgtgcatggt gatgccttca ggcgttttgt acaaggcctt gtagggcttg ccgtcagggg
     1261 atttggtcgg attgccagcc ttttccgggt tgccgggaca gatcttgacg cagggtgcgt
     1321 ccgagcaatg gttacacagc accgggatca ccgactgggt ggtgttggga aactttcctg
     1381 cagtgtgcat caggaagtct gcccaattga aactttgcaa atcaccccgg tcgcgggtgt
     1441 tgttttcagc tttgcaggca aaggcgcacg caccacagcc attgcatttt gcgaggtcaa
     1501 tgaccattcc aaattgtgtc atgatggttt actccagtgt cgggtgatgg ttcgatcagg
     1561 ctttttcgat gcgcacaccg gtaaagccgc cattgcgagc cgtggcaccg ctgaggcggt
     1621 catagtcatc caccagcact tcgttgttgt tgccaccgaa cgcaatcgcc ttgacaaaat
     1681 ctttggcagc aacccggcca taagcccagt gcccctggcc ataacatttg gccaccgtgc
     1741 ccgggcgcac gccttcccac agcttgagct ggcaagcaat cgtgccagcc accgaggtga
     1801 tcttcacgtt ctcgccatct ttgaggccca gcctggcacc atcgctgggg ttcatcttga
     1861 ccacgtcacc ccaagacaca tcgcccggat ccactttctt gaattcttgg taccaggtga
     1921 cgttggccga gcggccttcg cggttcaggc gcgatttgtg atcgatgaag gtgaacgggt
     1981 attccgccac actgccgtgg cgtttgggtg gttcatagtg cggcacaaag gccaggtcac
     2041 cgcgcgccac ataaccgctg acggtcatga tgtcgtcggt ggtggtctcg tacttcttgg
     2101 cgtgttccag caggcccttc ttgagggttt cgctgtagaa ctcaaacttc ttggtgactg
     2161 tgccaaactt gccgccccag ccctttttaa gggtgtattt agggccgctg aacatgccct
     2221 tggccttgaa ttcggcccag cccttgatgg gtgcgtcgcc tttgaggggc tccttgggca
     2281 tccacaatgt ggcgctactg atcttggccg caatttcgct gaactccatg gcggtggtgg
     2341 gcagcttgcc ggtctcggga tccttgaact ctttggagta gtagtcaaac agattgggga
     2401 aacctttggc tttgagtttt tcggccagca gccacatgac ttcagtttct tcctgcttca
     2461 catcccacag gcgtttgacg gcaccttgct ggatcgaggc gtagccgtga ccgttaccca
     2521 tgttcgacac aatcgaccag ccctctgctg aattgaacgt ggaaggcagc acgatgtcgg
     2581 caaacattgt catctcggag gcattgggca ccatgtgcac aaagaaggga atcttggcca
     2641 ttgctttgtc ccaacggcca gcgccagtgg cggagaaatt gaagttggac cacgatgaca
     2701 agaatacctt gacggctccc ggatctttca gcagaccatt ggctacgttg ttggtcacca
     2761 caccactgcc gggcacggca ttcatcatgg cgggcatgtc tttggctcca cgaccatcga
     2821 gtttcttgcc tttgctaccc gctttggcga catcatcgac atatttgtcc atgctgggga
     2881 acttggccag aggtacgctg ctgctctgcc acacaccacc ttcggtatca atcgagccaa
     2941 gcagaccatt gagcgcatgc acggccatgg cggcgtaagt gccgcgcggt gtcatggcaa
     3001 cacctggccc catccagacg gtggtttttg aaccggcttt acccatggca cgcgccaccc
     3061 gcagaatctg atcggcagga atcagtgttt ccttttccgc ccaggcgggg gttttgtcct
     3121 tgagctccag attccaccat ttgacgacac cgttggattc tttctctgca aaagccgctt
     3181 catccaccag cttgccagtc acaaacaggt ttttgccgtc tttgaagtcg cccacaaact
     3241 ccttgcccca ttgcccctcg gtaagaatga catgggcgat ggcgctcgcc agtgcaccgt
     3301 cggtgccggg gttgatgggc agccactcat gcgctttggc ggcgattgtg gacatgcgcg
     3361 ggtccacagc gatcaccgta ccgcgtttga cgatgccggg gaagtggtgc atggtgttgg
     3421 gcaccatgcg gttggagctt agcgggtcgc aaccccaaac caccagacag ttggtgttgc
     3481 ccaggtcgta gtcacggtag ccaaaaaagc cttgtgtcag gcctggaccc attttttcgg
     3541 cctcggcaca aagggcgctg tgtgagaaat actggcccgt gccccatacc ttgggcagcg
     3601 tgccgtacag caattctgtg gaggtagagg agtaccgtcc gcgcatgtac atgagcttgc
     3661 cgggctcgcc ggccttgcgc agttccatca tcttgtcagc cacggtgtca agggcttcat
     3721 cccaggagat cgggacaaac ttgggatcga taccccggcc cttgagcggg ttggtccgct
     3781 tcatcggtgt cttgatgcgg tcggggtcgt acatctgctg cggaatcaga tgaccgcgcg
     3841 ggcagcagta gccgtggttg gacttggaca gcggattgcc acgcacccgt gtggcacgac
     3901 cgtcttgcac aaagatctga accgcacacc attgcgtgca gccctggcag gtgctggcca
     3961 cccaattgcc agccgccggg ctgcagggct ttgccttgcg cgggatgagt gaactgtgga
     4021 cgggtcctgg
//
LOCUS       DAOSLL010000465         4029 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19683_length_4029_cov_8.843986, whole genome shotgun sequence.
ACCESSION   DAOSLL010000465 DAOSLL010000000
VERSION     DAOSLL010000465.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19683_length_4029_cov_8.843986"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(161..850)
                     /gene="fabR"
                     /locus_tag="PLB25_17090"
     CDS             complement(161..850)
                     /gene="fabR"
                     /locus_tag="PLB25_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HOE43325.1"
                     /translation="MITASPPSKRADTLTRSERKDLTRASLLQAALALIGEGRSFTSL
                     GIREIAREAGMVPNAFYRHFRNTDDLGLALVEETGVTLRRLLREARQVGVAPNSMVRR
                     SVQVYYEYVRQNRLQFLFISSERSGGSRILRLAIRTDVTHFTNEMAQDFRRLGIYPEM
                     PTSSLQMVCGLIVTTMLAAAPEILDLPPEQPLLEAEMKENFVQQLQVALLGAACWKEK
                     KVRQNTDIISL"
     gene            1033..1671
                     /locus_tag="PLB25_17095"
     CDS             1033..1671
                     /locus_tag="PLB25_17095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43326.1"
                     /translation="MIAANHTVPSTRWVETMVEAVKAALHHTRVAPVAPRPAASNALP
                     SLPPIPHLQQTVKNGELPTFSQVNSQLELPATLPARMLLLSKDSGIVATMSMLRDLQA
                     RNYQGDVVLLHVCRNPAELSQASALQRAADSYPELSLLVHFDDHAGPFSAKALTLAVP
                     DVKERTTWMCGPTGFIETVQAYWRSQRLTMPLHIGRCVTFPIQPMPPETVTA"
     gene            1793..2134
                     /locus_tag="PLB25_17100"
     CDS             1793..2134
                     /locus_tag="PLB25_17100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43327.1"
                     /translation="MTNVAAEPSLRFHHSSDLRAKTNAVLEALQARPDQPKHGESMAD
                     LVHELIEAGLEYYFLRALKKAQLGFVAEQSARLGMSGAVRMISSVSRQFIVRMDRQQL
                     LVVSEHIRELY"
     gene            2238..3926
                     /locus_tag="PLB25_17105"
     CDS             2238..3926
                     /locus_tag="PLB25_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE43328.1"
                     /translation="MEAIWLKSYPPGVPAEVDMFEFASLTDVLLRSCHRFAALPAYSN
                     METSISYAELDRSSRDFAAYLQKSLGLRKGERVALMMPNLLQYPVALFGVLRAGLVVV
                     SINPQYTADELEHQLKDSGAVAIVVLENFAHTLQKVLEKNPALNLTVITTEVGDLFAT
                     VKKLLTNIVVKHIKQMVTKWTIGGTTPFNAALRSGQKQDLTEEPLGQKDLAFLQYTGG
                     TTGVAKAAALTHGNLVANVQQMTAWMARDLVDGKEVFVCPLPLYHIYALTSNLVFMKL
                     GARTVLVTNPRDMRAYIHDLKKFPFTVIIGVNTLYRTLLDAPEFADVDTSALKVTSAG
                     GMAVQRVVAERWKQRTGVTIIEGYGMTETSPIVIANPLDTGEWTGTIGIPIPSTQAVI
                     LDEGDDQLATGEIGEICVRGPQVMPGYWNCPEETSKVFTTDGWLRTGDMGFVDERGYF
                     RITDRKKDMIIVSGFKVFPNQIEDIVSLHPGVAEVVAIGVPDERSGEVVKIVVVKRDP
                     ALTEQVLLDHCRQHLTGYKMPKIVEFRREPLPKSNLGKFLRRELRNAAAGALVP"
BASE COUNT          918 a         1166 c         1120 g          825 t
ORIGIN      
        1 cgggtctgaa gcgggtatat aggcaaccat aatattttca atgtttgcca ccactcctga
       61 agggtacgta taaatgggca atcgtccatt atgaagtgca ccgattggca aacgggtgtg
      121 ccaagattac tgatattcag gacgttcacg aaagtcccac ctacaagctg atgatgtctg
      181 tattttgacg aacttttttc tctttccagc acgcagcacc gagcagcgca acttgcaatt
      241 gctggacaaa attttctttc atctcagctt ccagcaaggg ttgctcaggg ggcaggtcca
      301 aaatctcagg cgcggcggcg agcatggtgg taacgatcag gccacagacc atttgcaggc
      361 tgctggtcgg catctcgggg taaataccca agcgtcgaaa gtcctgcgcc atctcgttgg
      421 tgaaatgggt gacatcggtg cggatggcca gccgcaggat acggctaccc cccgagcgtt
      481 cgctggagat gaacaagaac tgcaaacggt tttgccggac gtactcgtaa tacacctgca
      541 cggatcggcg caccatcgag tttggcgcca cacccacctg gcgcgcttcg cgcagcaggc
      601 gacgcagcgt tacacccgtc tcttccacca gggccagccc caggtcatcg gtgttgcgga
      661 agtggcggta aaaggcgttc ggaaccatgc ccgcctcacg cgctatctca cgaataccta
      721 gactggtaaa gctgcgcccc tcaccaatca aagccagcgc cgcctgcaac aggctggcgc
      781 gcgtcaggtc tttgcgctcg ctgcgagtca gggtgtctgc gcgtttgctg ggtggcgatg
      841 ctgtgatcat tttttctggt gttctttgtg tacaaacgta tcctatcaaa ttcaaatgaa
      901 tttcagcaaa agttaaacct ttgagtgtta gaaattggtg tccacgttta aaagcgaaaa
      961 actgcatgga catttagtgt acgaacgtac acaatattga cactctccaa cccaccaggc
     1021 aaggacacca tcatgatcgc cgccaaccac accgtcccat caactcgctg ggttgaaaca
     1081 atggtcgaag ccgtcaaggc tgcactgcac cacacgcggg ttgctccagt cgcaccccgg
     1141 cctgctgcaa gcaacgcttt gccatcgctg cctcccatcc cccatctaca acagactgtc
     1201 aagaacggcg agttgcccac cttcagtcag gtcaacagcc agcttgaatt gcctgcgaca
     1261 ttgcccgcca gaatgttgct gttgagcaag gacagtggca ttgtggcgac gatgtccatg
     1321 ctgcgtgatt tacaggcgcg caactaccag ggtgacgtgg tgttgctgca tgtgtgccgt
     1381 aacccggcag aattgagtca ggccagcgcg ttgcaaaggg ctgcagacag ctacccggag
     1441 ctttctctgc tggtccattt cgacgaccat gccggtccgt tctccgccaa ggccctgacg
     1501 ctcgctgtac ccgatgtgaa agagcgcacg acctggatgt gcggccccac cggcttcatc
     1561 gagacggtgc aagcttactg gcgcagccaa cgcttgacca tgccactgca tatcgggcgt
     1621 tgtgtcacct tccccatcca acccatgccc cccgaaacag tgacagcatg acctgcatca
     1681 ccttaggccc gagaccatga caacatcctc aaaaacaccc gtcgccagca cagacaaagc
     1741 agtcgccaaa cccgccacga agaaaacggt aagccacgca gcaagcaaaa ccttgaccaa
     1801 cgtggccgct gagccctctt tgcgctttca ccactcctcg gatctacgcg ccaaaacgaa
     1861 tgccgtgctg gaggcgctgc aggccaggcc agaccagccc aagcatggcg aatcgatggc
     1921 agatctggtc cacgaactca tcgaggcagg cctggaatac tattttttgc gagcgctgaa
     1981 aaaggcccaa cttggcttcg tagcagaaca gtcagcgcga ctgggtatga gtggcgcggt
     2041 acgaatgatc agttccgtca gccggcaatt tatcgtgcgc atggaccggc agcagcttct
     2101 ggtcgtatct gagcatattc gcgagctgta ttgaaccttg cctgacacgc caagcgcgtc
     2161 agcacccact gaattctcat cgggcgacat ccaggtttat ccaggcgatg gacccgaaac
     2221 tgcaaagaaa gatcaagatg gaagcgatct ggctgaagag ctacccgccg ggcgtgccgg
     2281 ccgaggttga tatgtttgag ttcgcctcgc tcacggacgt gttgctgcgc agctgtcacc
     2341 gtttcgcagc gttacccgcc tacagcaaca tggaaacgtc gatcagctat gccgagctcg
     2401 accggtcaag ccgcgacttt gccgcctact tgcagaaatc gctggggctg cgcaaaggcg
     2461 agcgggtcgc tctcatgatg cccaacctgc tccagtaccc ggtggccttg ttcggtgtgc
     2521 tgcgggcagg gcttgtggtc gtgagcatca acccgcagta caccgcagac gaattggagc
     2581 accagttgaa ggactcgggt gccgtggcca ttgtggtgct ggaaaacttt gcgcacaccc
     2641 tgcaaaaagt cctggaaaag aatccggcgc ttaacctgac cgtcattacg actgaggtcg
     2701 gcgacctgtt tgcgactgtg aaaaagcttc tcaccaacat cgtggtgaaa cacatcaaac
     2761 aaatggtgac caaatggacc attggtggca ccaccccatt caacgcggct ttgcgctccg
     2821 ggcaaaagca ggatttgaca gaagagccgc tgggccaaaa ggatctggcc ttcctgcagt
     2881 acaccggcgg cacaacgggc gtggccaagg ctgcggcgct gacccacggc aacttggtgg
     2941 cgaatgttca acaaatgaca gcgtggatgg cgcgcgacct ggtggacggc aaagaggtgt
     3001 tcgtctgtcc tttgccgctg tatcacatct atgcgctgac ctccaatctc gtgttcatga
     3061 agctcggtgc acgcacggtt ctggtgacca acccccgaga catgcgtgca tacatccatg
     3121 acctgaagaa attccctttt acggtcatca tcggtgtcaa tacgctttac cggaccttgc
     3181 tcgacgctcc cgagtttgcg gatgtggaca ccagcgccct gaaagtgacc agtgctgggg
     3241 gcatggccgt gcagcgcgtg gtggccgagc gatggaaaca gcgcaccggt gtgaccatca
     3301 tcgaaggcta cggcatgacc gaaacgtcgc cgattgtgat cgccaacccg ctggacaccg
     3361 gggagtggac cggaacaatc ggcatcccga ttccgtcgac ccaggccgtt atcctcgacg
     3421 agggcgatga ccaactcgcc accggcgaaa tcggtgagat ctgcgtgcgc ggcccacagg
     3481 tgatgccggg gtactggaac tgccccgaag aaaccagcaa ggtgttcacc actgatggct
     3541 ggctacgcac cggcgatatg ggctttgtgg acgaacgggg ctatttcagg atcactgacc
     3601 gaaagaagga catgatcata gtttcgggct tcaaggtctt cccaaaccag atcgaggaca
     3661 tcgtctcgct gcatcctggt gtggccgagg tcgtggcgat cggcgttcca gacgaaagat
     3721 ccggtgaggt cgtcaagatc gtcgtggtga agcgcgaccc ggcgctgacc gagcaggtct
     3781 tgctcgacca ttgccgtcag cacctgactg gctacaagat gccaaagatc gtcgaattcc
     3841 gaagggaacc cctgccaaag tccaacctcg ggaaattcct gcggcgagag ctgcgaaatg
     3901 cagcagctgg tgctttggtg ccttagccag tttctattgc aacactgcgt gaacttgact
     3961 ttcacaggca gatttgaacg accggtttgg atgaggcatt ttggtatgct ggctgaccgc
     4021 tgtgggtct
//
LOCUS       DAOSLL010000466         4010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19796_length_4010_cov_3.700379, whole genome shotgun sequence.
ACCESSION   DAOSLL010000466 DAOSLL010000000
VERSION     DAOSLL010000466.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19796_length_4010_cov_3.700379"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            109..3273
                     /locus_tag="PLB25_17110"
     CDS             109..3273
                     /locus_tag="PLB25_17110"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain repeat protein"
                     /protein_id="HOE43329.1"
                     /translation="MKSNLSGTRAHLGRWMHMAAALCISLSLAACGGGGGGDSPNAAS
                     PNAMKMAATSALVTPDVVVTGITKLSETRVSRTVYDYLFQVAVKNNGTDAKSGVAATL
                     TGVGIGATIVDGTVQVGNLAAGATVTLNDTITIRQDRTQPFIQSALTWQVTAVSIPPT
                     ISGVLLTGDPSQPADQATIDFVDQSPEDLATAQASGSGAEYYVSQLAAFISPGATVGQ
                     VNTVLNSISGRISYMRQNETAVTIHVPSPGSLAALQLIASNLMATGVFEAVIPNLVPQ
                     PQNLPINVIPADAFLPVNPVSHQNPVFHHIAARVTAAWNAVNVNTATSPVELIVIDYF
                     GNDQITEINGTAVGILSGNKCNKYNLEGAFIGIETCTHGYHVLGIAAGSFGGDFTATG
                     EVTGSLPKAIPLHVIDLAGPIGPPEPFVQLFNRRMREIITAAKPNRRFVINMSVGTNC
                     PIPGDCLTASEAEINAKYWRKTVRNLSLKLPAGVNFNYENQVIQVSSAGNNGPTSHAE
                     RTSWWNAAVLLGPLTDKNGAISVPLTNGLVVENRTAEISLDPLKGLIVVPGPLNKFEI
                     INKDGTKSFGGSTIGGNIGAIGTNIYSFISPTEVGIKSGTSMASPQVAGIAAYMLAIR
                     PTISPVDLISRLKGTGQAPLAGAAPMMDAFAAILSLDNSFVDAPVRLAMLQPNVTTPK
                     STFGSSDASKFLQVFFPAAYPQIPALPSQPNFSQFDLNGDGFTGDDSKKSAFNLDFSP
                     GLSPSGLILNYEDGGEIQLDRAQVTDFEVLCYYVHSRLFDAGGLTAFNAELTRIAGEV
                     KRPVSCDPPKAKSFASISALPTSVVVNLPVMFTSVVRPTPDLGQSLPTGNVTFTTEGG
                     ATLCSATLDASGTGSCSAPLTTTGITNVFANYSGNSNYLPTKSVGVSIMVGCGGGADT
                     ILAQGTWGTIKRKGCAALKRGGSVAFTDLIEYTKGTDGASWGYVNIYAPGSTSFSLSG
                     GFMPPGYGNAYVIGNAWGSGNADPACMAGTADAPFYSSIRITVPSTYSGNSISIGTIY
                     FRYNRNCFIYAAGNDGAITFPVID"
BASE COUNT         1005 a         1060 c         1020 g          925 t
ORIGIN      
        1 cgttgcttct cgattttcga cgtcagtttc ctcgcaaata ccgaaattag caaactattc
       61 actcaacgtc atgccacgca tggcaaaaaa ttcatgtggg ggtgaaaaat gaaatccaac
      121 ctatcaggta cacgtgcaca ccttggccgc tggatgcata tggcggcagc gctttgcatc
      181 tcgttgtcac ttgctgcatg cggtggtggc ggcggcggtg actcacccaa tgcagcgagt
      241 cctaacgcca tgaaaatggc agcgacttca gctttggtga cgcccgatgt tgtcgtgact
      301 ggcatcacaa aactgagcga aacccgcgtg agcagaacgg tgtatgacta cctttttcag
      361 gtcgcggtaa aaaacaatgg cacagacgca aaaagtggtg ttgcagcgac gctgacaggc
      421 gttggtattg gcgcgaccat agtggatgga acagtgcaag tgggcaatct ggcagctggt
      481 gcgacggtga cgctgaatga cacgatcacg attcgacagg atcgaactca gccatttatc
      541 caatctgctc taacatggca ggtaactgcg gtgtcaatac cacccactat ttctggcgtt
      601 cttttgaccg gggatccaag tcagcccgct gaccaggcca cgatagactt tgtggatcaa
      661 agtcctgagg atctggcgac agcgcaagcg tctggatcgg gcgcggagta ttacgtatct
      721 caactggctg cgtttatcag ccctggcgcg accgtcggcc aagtgaatac ggtgctgaat
      781 agcatcagtg gccgaattag ttacatgcgc caaaatgaga ccgctgtcac catccacgtc
      841 ccgagccccg gaagtctagc ggcccttcaa ttaatcgcct ctaatctcat ggctactgga
      901 gtttttgagg cggtgattcc gaacttagta ccacaaccac agaatcttcc aataaacgtc
      961 attccggctg atgcgtttct gcccgtaaat ccagtgtccc atcaaaatcc agttttccat
     1021 cacatagccg ctcgcgttac ggctgcgtgg aatgccgtca acgtcaacac cgccacgtcg
     1081 ccggttgaac tcattgttat cgattatttc gggaatgacc aaattacaga aattaacggt
     1141 acggcagttg ggattctttc aggaaataaa tgcaataaat acaatcttga aggcgctttt
     1201 attgggatag aaacttgtac acatggatat catgttctcg gcatagccgc tggtagtttt
     1261 ggtggcgact ttacagctac aggagaagtg acgggcagtc tgcccaaagc gataccgttg
     1321 catgttattg acctcgcagg tccaattggt ccaccagagc cctttgtgca gctcttcaac
     1381 agacggatga gagaaatcat caccgctgct aagccgaata gacgcttcgt catcaatatg
     1441 agcgttggta caaattgccc gatccccggg gactgtttaa ccgcttctga agcggaaatt
     1501 aacgcaaagt attggcgtaa gacggtccga aacctcagtc taaaactccc tgctggggtt
     1561 aacttcaact acgaaaacca ggttatccaa gtatcttctg caggcaacaa cggacctacc
     1621 tcccatgcag aaagaaccag ttggtggaat gcggcggttc tgctgggtcc tttgactgac
     1681 aagaatgggg caatatctgt gccccttaca aatggtctgg tggttgagaa caggactgca
     1741 gaaatttcgc tggatccttt gaaaggcttg attgtcgtgc ccggaccact taataaattt
     1801 gaaattataa ataaagacgg cactaaaagt tttggaggta gcacaatagg cgggaacatc
     1861 ggtgcgatcg gcacgaatat ttactcgttt atatccccga cagaagtcgg cattaaaagc
     1921 ggaacttcaa tggcgagccc acaggtcgcc ggcattgccg cttatatgct tgccatccgt
     1981 ccgacaatca gcccagtgga tctgatttct agactcaaag gtacgggcca agcgccacta
     2041 gccggtgccg cgccgatgat ggatgccttc gccgcgatat taagcttgga caatagtttt
     2101 gttgacgcgc ctgttcggct tgcaatgctt cagccaaacg tcacgactcc aaaatcgaca
     2161 tttggctcct cagacgcatc aaaatttctt caagttttct tcccggctgc ctacccccag
     2221 ataccagcat taccgagtca gccaaatttt tctcaatttg atctgaatgg ggatgggttt
     2281 actggcgacg attctaagaa atcagcgttc aacctcgact tctcgccggg tctctctccc
     2341 tcgggactga tcctcaacta tgaagatggc ggggagattc aattggatag ggctcaggtg
     2401 acggatttcg aggtactttg ctactacgtc cacagtcgac tgttcgatgc tggcgggctt
     2461 acggccttca atgcagaatt gacgcgcata gccggtgaag ttaaacgtcc cgtatcttgt
     2521 gatcctccaa aggccaaatc ctttgcatct atatcagcac taccaacttc agtcgtagtg
     2581 aacttacctg tcatgttcac gtctgtcgta aggccaacac cggatcttgg acaaagtctt
     2641 ccaactggta acgtcacttt caccacagaa ggtggtgcga cgctttgtag tgcaacactt
     2701 gatgcgtccg ggacgggttc gtgctccgca cctctaacca caactggtat cacgaatgta
     2761 tttgccaatt actctggaaa ttcaaattac ctgccaacca agtcagtggg agtgtctatc
     2821 atggtgggct gcggaggcgg cgcagacacg atcctagcgc aaggcacctg gggcaccatc
     2881 aagcgaaagg gctgcgcggc gctcaaacga ggcggaagtg tcgcctttac cgacctcatc
     2941 gagtacacga agggcactga cggggcgagt tggggctacg tcaacatcta tgctcccggc
     3001 tccacctcgt tctcgctcag cggcggattt atgccacccg gatacggcaa cgcatacgtg
     3061 atcggtaatg catggggtag cggcaacgct gatccggcgt gcatggcagg aacggccgat
     3121 gcgcccttct attccagcat ccgtataacg gttccgtcca cctatagcgg caactcaatc
     3181 tcgatcggga ccatctactt ccgctacaac cggaactgct tcatctatgc ggccggcaac
     3241 gacggcgcaa tcaccttccc cgtgatcgat tgaaataggc gcgcacttcg tccaaggttg
     3301 tggccagtta ggaggggacg acacacgcgc cgctccgtac cgacctcccg ggctccggct
     3361 ggagtcgcgg cacaaacgga tgaggggatg tgagcgcgtg atcaatatga tcaacaaggg
     3421 cgagacagga gcggcgtttg ccctcatcaa agtccaaacc ctaaccacat cgaccgctgg
     3481 cactctgagc acaatgccgg gcctgggtac tgcacgaagc gatgacgctt agttgagatg
     3541 acaagtcagt tgtcaccagc tccgccgctt ccgaatacgt cagggcagtc aaaccactgg
     3601 cacaaggccg cccatgcttg aggccaacgt cgcgggtcac gcgacgcagc atgaaggttg
     3661 gcggtgagat acagcagcat gagattgaat aatgctatgt cgctgaccaa tagtcaccct
     3721 tactaaaaat caacacaggc gtattactga catcgcgttg ttaaacgcca acggtgtgag
     3781 ataccaaatc accggccgtt ttgtacagta atattattcg acactgacat ttgtgtgatg
     3841 tcgcacaaga cttcaactta acacaagccc cgtcgcatca aaaacgatgc cagccaaatc
     3901 gtgatgccca aggtcggcta tctggaaggc atttcggcaa actggctgac agcaacgggt
     3961 ctgaagcggg tatataggca accataatat tttcaatgtt tgccaccact
//
LOCUS       DAOSLL010000467         4002 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19850_length_4002_cov_3.931847, whole genome shotgun sequence.
ACCESSION   DAOSLL010000467 DAOSLL010000000
VERSION     DAOSLL010000467.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19850_length_4002_cov_3.931847"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(80..1009)
                     /gene="xerD"
                     /locus_tag="PLB25_17115"
     CDS             complement(80..1009)
                     /gene="xerD"
                     /locus_tag="PLB25_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HOE43330.1"
                     /translation="MKEIHHPRIAARSDPVIDTFIDALWLEEGLAKNTLSAYRLDLTL
                     LSDWQTVNARALIGTRTEDLNGYFAQAHSQTKATTANRRLTVFKRFFRWALREHLIDQ
                     DPTLKLQAAHQPLRMPKTLTEFQVEALLGAPDVSTALGVRDRSMLELMYASGLRVSEL
                     VTLKVFNISLNDNVLRVFGKGNKERLVPFGEVASLWLKNYLAQARAELLAGHQTDDLF
                     ITVKGSTPGTAMSRMMFWLLVKKYALVAGITSPLSPHTLRHAFATHLLNHGADLRAVQ
                     MLLGHADISTTTIYTHVARERLKVLHARHHPRG"
     gene            995..1906
                     /locus_tag="PLB25_17120"
     CDS             995..1906
                     /locus_tag="PLB25_17120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HOE43331.1"
                     /translation="MNFFQLLFPDFLLILIGYLVCRFTPLNRPIWSQVEALVYFLFFP
                     VLLFHSIVRSPLDLGAAGNLVAAGWSLGLGGIALAYSVPYWPGLGRHIDQRQHAASAQ
                     VAFRFNSFIGLALAERLAGPEGLLVLAILIGISVPLMNIGAVWPMARHAGHGFWRELT
                     RNPLIIGTASGLLANLLGLSIPHWLEPTVTRIGSASLSLGLMAAGAGMQLASLQRDKL
                     LGLALLGIRHFLMPLLAILLARLFSLNPLQTTVLLAFSALPTASSCYVLAARMGYDGA
                     YVAALVTLSTVLGLASLPFALAVLNKL"
     gene            1969..2562
                     /locus_tag="PLB25_17125"
     CDS             1969..2562
                     /locus_tag="PLB25_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007864829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HOE43332.1"
                     /translation="MNTTHLLYLHGFLSSPASTKARLTAAAVAARHPTVTWLCPMLAP
                     SPQQAMAEVLAAVADWPRHAMAIVGSSLGGFYATWLAERLGCPAVLLNPAVHPARDLA
                     PHIGKQTLWHDPDRHFVLEPGFVDELKAMELTGISRPERYFAIIARGDEVLDWREMTS
                     HYAGAKIKLLAASDHALSDYTQHLDEVLGFLALAQLR"
     gene            complement(3516..3896)
                     /locus_tag="PLB25_17130"
     CDS             complement(3516..3896)
                     /locus_tag="PLB25_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43333.1"
                     /translation="MAIGWLTILKMVPWGDVIENAPKVATGAKKLWSRVAKKPAGATV
                     IVAADAMPEQPLTLLQLQQQVTALQTVTAELQQQMIDSTELINTLAEQNSRLVKRVEI
                     DRKRLLWLALCLLAMLLVLLFKPF"
BASE COUNT          714 a         1178 c         1193 g          917 t
ORIGIN      
        1 gccgcgcatc ccaactcctt ggtgcaaaac ggggagttat tcccgaccac atcctgagcg
       61 cttcatgcca tccgtgacct cagccgcgcg gatggtgtcg ggcatgcagc accttgagcc
      121 gctcgcgtgc cacgtgggtg tagatggtgg tggtggaaat gtcggcatga cctagcagca
      181 tttgcaccgc gcgcaggtcc gccccgtggt tgagcaaatg cgtggcaaag gcgtggcgca
      241 gcgtatgtgg tgacagcggc gacgtgatgc ccgccaccag tgcatacttt ttcaccaaca
      301 gccagaacat catccggctc atggcagtgc cgggtgtgct gcccttgacg gtgataaaca
      361 ggtcatcggt ctggtgaccg gccagcagtt ctgcgcgtgc ttgcgccaga tagttcttca
      421 gccacaggct ggcgacttcg ccaaacggca ccagccgctc tttgttgccc ttgccaaaca
      481 cccgcagcac gttatcgttg aggctgatgt tgaacacctt gagcgtgacc agttcgctga
      541 cccgcaagcc gctggcgtac atcagttcga gcatgctgcg gtcacgcacg ccaagcgctg
      601 tgctcacgtc cggtgcgccc agcagcgcct caacctggaa ctcggtcagc gtcttgggca
      661 tgcgcaacgg ctggtgggcc gcctgaagct tcaaggtcgg gtcctgatcg atcaggtgtt
      721 cccgcagcgc ccagcgaaaa aaccgcttga acacggtcag acggcggttt gcagtggtgg
      781 ctttggtttg gctgtgggct tgcgcaaaat agccgttgag gtcttcggtg cgggtgccaa
      841 tcagcgcccg ggcgttgacg gtttgccagt ctgacaacag cgtcaggtca agccggtagg
      901 ccgacagcgt gtttttggcc agcccttctt ccagccatag cgcatcgata aacgtgtcaa
      961 tcaccgggtc actgcgggct gctatccttg ggtggtgaat ttctttcaac tgctgtttcc
     1021 tgactttttg ctcattctga ttggctatct ggtgtgccgc ttcacgccac tgaatcgccc
     1081 gatctggtcg caggtcgaag cgctggtgta ttttctgttt tttccggtgc tgctgtttca
     1141 ctcgattgtc aggagcccgc tggacctcgg cgcagccggc aacctggttg ccgctggctg
     1201 gtcgcttggg ctcggtggca ttgcgctggc ctactcggtg ccgtattggc ccgggctggg
     1261 gcggcacatt gaccagcgcc agcatgccgc cagcgcacag gtggcgtttc gtttcaactc
     1321 tttcattggc ctggcgctgg ccgagcggct ggccggaccc gagggtctgc tggtgctggc
     1381 aatcctgatt ggtatcagtg tgcctttgat gaacattggc gcggtctggc cgatggcacg
     1441 ccatgccggg cacggcttct ggcgcgaact gacacgcaac ccgctgatca tcggcaccgc
     1501 atcaggtctg ctggccaatc tgctggggct gtcgattccg cattggctcg aaccaacggt
     1561 gacgcggatt ggttcggcat cgctgtcact gggtttgatg gcggccgggg ccggcatgca
     1621 gttggcatca ctgcagcgcg acaagctgct gggtctggcg ctgttgggca tccgccactt
     1681 tttgatgcca ctgctggcca ttttgctggc gcggctgttt agcctgaacc cgttgcaaac
     1741 gaccgtcttg ctggcgttct cggccctgcc aaccgcatca agctgctatg tgctggcggc
     1801 gcgcatgggt tacgacggtg cctacgtggc agcgctggtc accttgtcaa ccgtgctggg
     1861 cctggcgagt ttgccgtttg ccctggcggt tctcaataag ctataaataa tatagctacc
     1921 agcgcagtat tgacgggcgc tagaggctgt ttttatataa ttttccagat gaataccacc
     1981 catttgctct acctgcacgg ctttctgtca tcgcctgcgt ccaccaaggc gcgcctgacc
     2041 gctgcggcag tggccgcgcg tcacccgaca gtgacctggc tttgcccgat gctcgcgcca
     2101 tccccccaac aagccatggc cgaggtgctg gccgccgtcg ccgattggcc cagacatgcc
     2161 atggccatcg tgggctcgtc tttgggcggg ttttatgcca cctggctggc cgagcgcctg
     2221 ggctgcccgg cggtgctgct caaccctgcg gtccatccgg cgcgcgacct ggcaccccat
     2281 atcggcaagc aaaccttgtg gcacgaccct gaccggcact ttgtgctgga gcctggattt
     2341 gttgacgaac tcaaagccat ggaactgacg ggcatcagcc gacccgagcg ctactttgcc
     2401 atcatcgccc gtggtgacga ggtgctggac tggcgcgaaa tgaccagcca ttacgctggc
     2461 gcaaagatca agctgctggc tgccagcgac cacgcgctga gcgactacac acaacacctc
     2521 gatgaggtgc tgggcttttt ggcgctggct caattgcgat gactgtgatc atcctggtct
     2581 ggcctgaaag tgctcaacgg gtattcgcaa tcgacttcgc agaaggcgat ttttcgtgaa
     2641 tcgatgccct cgtgaacgcc tgtcgggcat gggatctctg gcaaccgagc agttttgatt
     2701 tgcttaagcg cgacgaaccg gaatcgatga gccttcgtcg cgagggcgcg actcctacac
     2761 aaacaggcag cgcctatttg aatttgttgg agtcgcgccc tcgcgacgaa tgactttgaa
     2821 tgtttgaatt cttcgcatga tttgcaaaga cttggcttgt tggagattaa aggccagact
     2881 gttttgatca aaactccagg tttccctgtt cattcaccac cgttttccaa cttctgtgac
     2941 atttggaccc attaccgcca tgcgcttgac atgccagccc agaccggcca gatgtcattt
     3001 gccagtgacc ttgcctcatc cgcgcaacgg gcacgaactg aacctcatcg ctgatttgat
     3061 ggtgcaaaag aacagtgcca cttgttgctt gcaaatggca tcagccgccc tgcctgcttg
     3121 cctgcttgcc tgcttgcctg cttgcgcgct tgctcgcttg ctcgcttgcg tggtcaggcg
     3181 gtaaaccaca ccgattgcag cggtgccgca tctgccttga cgggtaaagg ctacgaacag
     3241 cgctcacgcc aggttggtgg ccagcgcctg cgctctgatg caaagggggc gcgcaggtgg
     3301 tgcgcaactg acggggggtg caaaggtggc aatcggagct tcgggccggt tgccctgcag
     3361 accattgacc cctgttggat cctgttggat cctgttggat cctggttgga ccccagttga
     3421 aacccggttg gaatcgcgtt ggcaagaccg ttatcaatgg gtgaaatgat gtccaaatag
     3481 gcttccagcc cttatggaat atgcggtgaa cgctatcaaa aaggtttaaa aagcagcacc
     3541 agtagcatcg ccagcaggca tagggccagc cacaacaggc gcttgcggtc gatttccacg
     3601 cgcttgacaa gacggctgtt ttgttcagcc agggtgttga tcagttcggt ggaatcgatc
     3661 atttgctgct gcaactcagc ggtgacggtc tgcagcgcgg tcacctgttg ctgcaactgc
     3721 aacagcgtca ggggctgctc aggcatcgcg tcggcggcga cgatgacagt ggcaccagca
     3781 ggctttttgg ccacccggct ccagagcttt ttggcaccgg tcgccacctt gggggcgttt
     3841 tcgatcacgt cgccccaggg caccattttc agaatcgtca gccaaccaat cgccatgcag
     3901 cacctcttgt aacaaacagg ctggcattgt ccaccacggc aggtttcgtg cgccaatggc
     3961 tcattgcaca ataccccttg cgaaaaccag gtgcgggtgt tg
//
LOCUS       DAOSLL010000468         3994 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19896_length_3994_cov_4.427774, whole genome shotgun sequence.
ACCESSION   DAOSLL010000468 DAOSLL010000000
VERSION     DAOSLL010000468.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19896_length_3994_cov_4.427774"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1820)
                     /gene="prsT"
                     /locus_tag="PLB25_17135"
     CDS             complement(<1..1820)
                     /gene="prsT"
                     /locus_tag="PLB25_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system TPR-repeat protein PrsT"
                     /protein_id="HOE43334.1"
                     /translation="MKFNQSNIRTASAAVVLSLLLGACSEKPEAMLSSAKDYLAKKDN
                     KAAVIQIKNALQVNPDLPEARFLLGSALLDGGDPIGAATELRKALALKYPPDALLPLL
                     AQALVAQGQAKKLIDELAGTELTDPLAKASFQTTLATAYAMQGKAELSQAALSAALQA
                     APSYAPAMVAKARQMAVNRDFEGAMALTEETISKNPNSVEAWKLKGDLLRYLKSQLPQ
                     ALVAYRKTVEIKPDFLAGQGAVITILLQQDNLAEAATQVEGLKKIAPNHPQTKYLEAQ
                     LAFQKKEYKPARELIQQVLKVAPNNIQSLQLAGAVELQLNSLVQAEVYLSKALQALPD
                     LALARRLLVMIYLRSGQTAKAIATLQPGLNRESVDPDLYAVAGEVYLQSGDAKKAETF
                     FALASKQDPGNARTRTSLALTRLAGGQVETAFDELHTIAASDPGTTADLALISVHLRR
                     GEFDKALTAIDSLEKKQADKPLAANLRGRTLLVKKDLPGARKSFERALVIDPGFFPAV
                     ASLAGLDLAEKKPDAAKKRFEAVLLKDPKNPQALLALADLAARSGAAKDEVAKLIGNA
                     VNANPTDVPPRLLLTEFHRRNKDIKLALSAAQDAVAALPN"
     gene            complement(1858..3219)
                     /gene="prsR"
                     /locus_tag="PLB25_17140"
     CDS             complement(1858..3219)
                     /gene="prsR"
                     /locus_tag="PLB25_17140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HOE43335.1"
                     /translation="MSSERSRPLLIVEDDLALQKQIKWSLDRFDSVLAHDRESALLQL
                     RKSNPAVVTMDLGLPPDADSVSEGFRLLGQMLAFDPDIKVIVLTGQNDQANALQAVAL
                     GAYDFFAKPFEPELLNLTVERAFRLFELQSENRRLQTMHQPDALSGLMTRDPAMMRVC
                     RTIEKVAVSNATVMLLGESGTGKELLARGLHQASARHSEKFVAINCAAIPENLLESEL
                     FGYERGAFTGASKTTLGKIEMANGGTLMLDEIGDLPFQLQAKLLRFLQERVIERVGGR
                     QEIAIDVRIVCATHQDLKVLSKEGRFREDLYYRLAEIVVNIPPLRDRVGDAALLAHAF
                     VRRFSQEQNRASMLLSEDAVKAIELHPWPGNIRELENCIKRATIMADGNQISAEDVGL
                     STAARDEIDTSLDLRIIRDTADRRAIIAALGRVNGNMVKAAELLGVSRPTLYDLMHRL
                     ALK"
     gene            complement(3216..>3994)
                     /gene="prsK"
                     /locus_tag="PLB25_17145"
     CDS             complement(3216..>3994)
                     /gene="prsK"
                     /locus_tag="PLB25_17145"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HOE43336.1"
                     /translation="NWEVNDLLKTAGQQAASYLAQMQATEALLEVRKFDAFNRMSAFV
                     VHDLKNIVTQLSLMMKNAKRLANNPEFQQDMLMTVENSLERMRQLMLQLREGALPPGT
                     VLGIDLSAVIARVKSVSAAKGRTLEVELQDSVSARGHEERLERIIGHVVQNAFEATDP
                     NGRVSLRLDRSAGQARIVVTDTGHGMSQEFIRDRLFKPFQTTKQAGMGIGAYESYQYV
                     QELGGKIQVESQEGKGTSVTIMLPLFENQKHSDLHVMEPQ"
BASE COUNT          820 a         1147 c         1091 g          936 t
ORIGIN      
        1 ctattgggca gggcagctac ggcatcttgc gcggccgaca atgccagctt gatgtctttg
       61 ttgcgcctgt ggaattcagt cagcaacagg cgcggtggca catcagtggg attggcattc
      121 acggcattgc caatcagctt ggcaacttca tctttggcgg caccagagcg tgccgcgagg
      181 tcggccagcg ccagcagggc ttgcgggttt ttcgggtctt taagcaacac ggcttcaaaa
      241 cgctttttgg cggcgtcggg tttcttttcg gccaaatcca gcccagccag gctggcgact
      301 gccggaaaga agccgggatc gatgaccagc gcgcgctcga aactctttct ggcacctggc
      361 agatcttttt tgaccagcaa tgtcctgccg cgcaggttgg ccgccagcgg cttgtcagcc
      421 tgtttctttt caagcgaatc gatggctgtc agggccttgt caaactcgcc acggcgcaga
      481 tgaacgctga tcagggccag gtcggctgtg gtcccgggat cagacgcggc aatggtgtgc
      541 agttcgtcaa aagctgtttc aacctggcca ccagctagac gtgtcaacgc caatgatgtt
      601 cgggttctgg cgttgccggg gtcttgtttg ctggccaagg caaagaacgt ctccgctttt
      661 tttgcatcgc cactttgcag atatacctcc cccgcgacag catacagatc aggatcaacg
      721 ctttcacggt tcaacccggg ttgcaaggtg gcaatggctt tggcagtctg gcccgagcgc
      781 aggtaaatca tcaccagcaa gcgtcgcgcc agggccagat caggcaaagc ttgcaaggcc
      841 ttgcttaaat acacctcagc ctgaacgagt gaattgagct gcagttccac cgcaccggcc
      901 agttgcaagg actgaatgtt gttgggggct accttgagta cctgctggat cagttctcgg
      961 gcaggcttgt attccttctt ttgaaaggcc aactgggctt ccaggtattt ggtctgcggg
     1021 tggttgggcg cgattttttt cagaccttcg acctgcgtcg cggcttctgc caggttgtct
     1081 tgctgcaaca agatggtgat gacggcgccc tgcccagcca ggaagtctgg cttgatttcc
     1141 accgttttac ggtaggccac cagcgcttgc ggtaactggc tcttaagata gcgcagcaaa
     1201 tcgcccttga gtttccaggc ttcaacactg tttgggttct tgctgatggt ctcctcggtg
     1261 agcgccatgg caccttcaaa atcacgatta actgccattt gccgtgcttt ggcaaccatg
     1321 gctggcgcat aggatggtgc cgcctgcagt gccgcactga gtgccgcctg cgacagctcg
     1381 gctttgcctt gcatcgcata ggcagttgcc agagtggttt gaaaactggc cttggccaat
     1441 gggtcggtca actcggtgcc agccaattcg tcgatgagct ttttggcttg cccttgcgcc
     1501 accagcgcct gagccagcaa aggcaacaac gcgtctggcg ggtatttgag ggccagggcc
     1561 ttgcgcaact cggtcgcggc accaatcggg tcgccaccat cgagcagcgc gctgcccagc
     1621 aaaaaacggg cttccggcag gtccgggttg acttgcaagg catttttgat ctgaatgaca
     1681 gcggccttgt tgtccttttt tgccaaatag tcttttgcag aactcagcat ggcctcaggc
     1741 ttttcgctgc aggcacccag taacaggctc aacacgactg ctgctgacgc agtgcggata
     1801 ttagattgat tgaatttcat cgagactccc tgcaaaaaga ttacaaaaac gtatggatca
     1861 tttgagtgcc agccggtgca tcaggtcata aagtgttggc cgactcacac ccaacagttc
     1921 ggcagctttg accatgttac cgttgacccg gcccaatgca gcaattatgg cccgccggtc
     1981 ggcggtgtcg cggatgatgc gcaagtccag cgaggtgtcg atttcgtcgc gggcggcagt
     2041 gctcaacccg acatcctcgg ccgagatctg gttgccatcg gccatgatgg tcgcgcgctt
     2101 gatacagttt tccagctcgc ggatgttgcc cggccaagga tgcagctcga tcgccttgac
     2161 ggcatcttca ctcagcaaca tcgaggctct gttttgctct tgcgaaaagc gacgcacaaa
     2221 ggcgtgcgcc agcagcgctg cgtcgcccac ccggtcgcgt agcggcggaa tgttcacaac
     2281 gatttcggcc agcctgtaat acaaatcctc ccggaaacga ccctctttgc tgagcacctt
     2341 caagtcttga tgggtagcac aaacaatgcg cacatcaatt gcaatctctt gccggccacc
     2401 gacgcgctca atcactcgtt cttgcaaaaa gcgcagcaac ttggcctgca actgaaaagg
     2461 cagatcaccg atttcgtcga gcatcagcgt gccgccgttc gccatttcaa ttttgcccag
     2521 tgtcgttttc gaggcaccgg taaaggcccc tctttcgtag ccgaaaagct cgctttccaa
     2581 caagttctcc gggatggcag cgcaattgat ggcgacaaat ttttcgctgt gccgcgcaga
     2641 tgcctggtgc agtccgcgcg ccagcaactc tttgccggtg ccgctttctc ccagcagcat
     2701 gacggtggca ttgctcacgg ccactttttc aatggtgcgg cagacccgca tcatggctgg
     2761 atcacgggtc atcaggcccg acagggcatc gggctggtgc atggtctgca agcgccggtt
     2821 ttcgctctgc aactcaaaca gccgaaacgc acgttcaacc gtaagattga gcaattcggg
     2881 ttcaaacggc ttggcaaaaa aatcgtatgc acccaatgcc acagcctgca aggcattggc
     2941 ttggtcgttt tgcccggtca ggacgatcac cttgatgtcg gggtcaaaag ccagcatttg
     3001 tccaagcaac ctgaagcctt cagacaccga atcggcatct ggtggcaatc caaggtccat
     3061 ggtgaccaca gccgggttgc tcttgcgcaa ttgcagcaag gcactttccc gatcatgggc
     3121 aagcacggag tcaaaacggt caagcgacca cttgatctgc ttttgcagcg ccaggtcatc
     3181 ttccacaatc agcagggggc gggatcgttc tgaactcatt ggggttccat cacatgaagg
     3241 tctgagtgtt tctggttttc aaacaaaggc agcatgattg tgacgctggt tcccttgccc
     3301 tcttggctct caacttgaat cttgccaccc aactcttgca catactggta gctttcataa
     3361 gcgccaatac ccatgccagc ttgtttggtg gtctgaaagg gcttgaaaag tcggtcacgg
     3421 ataaactcct gactcatgcc atgtccggtg tcggtcacca caattcgggc ttgccccgct
     3481 gatcggtcaa gtcgcaagct gacacgacca ttggggtcgg tcgcctcaaa ggcgttttgc
     3541 accacatggc caatgatacg ttccagtcgc tcctcatggc cgcgggcaga cactgaatct
     3601 tgcagctcca cttccagtgt tcgccccttg gccgcagaaa cgcttttgac gcgagcgatg
     3661 acggcactca ggtcgatacc cagcacggtt ccgggcggca aagcaccctc gcgcagttgc
     3721 aacatcaact gacgcatgcg ctccagcgaa ttttcaaccg tcatcagcat gtcttgctga
     3781 aactctggat tattggccag gcgtttggca tttttcatca tcaacgacaa ctgcgtcacg
     3841 atgttcttga gatcatgcac gacaaaagcc gacatacggt tgaacgcatc aaacttgcgc
     3901 acctccagca gcgcctcggt cgcttgcatt tgcgccaggt agctggctgc ctgttgcccg
     3961 gcggttttca ggagatcatt gacttcccag ttca
//
LOCUS       DAOSLL010000469         3989 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19928_length_3989_cov_6.572445, whole genome shotgun sequence.
ACCESSION   DAOSLL010000469 DAOSLL010000000
VERSION     DAOSLL010000469.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19928_length_3989_cov_6.572445"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(22..1425)
                     /locus_tag="PLB25_17150"
     CDS             complement(22..1425)
                     /locus_tag="PLB25_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43337.1"
                     /translation="MSPAAAQDLRADCNSCRMVVHSLAHPLPLAGNWLFTRNDNPHNK
                     DVALDTQNWQLVQAPGPWRNVYPDNKNFTVGWYRGTFEFAPDLIGQEVVLLVDAYMGR
                     VNVYIDGQEVYKRPHNINVERYYSIQPIPVRFIVKQAQQVVTIRVDTPLMTGIYQLPF
                     ELRKYDAHDRGLAWEQFWGGELRTIVAYVVFFFGLFLLLVYAKTRSRMYLIAALGSLA
                     SFPFYAAPADYLLRFFAPEPLLFVHYFGIFSYFLAYVFCQFYYRFTPRLNWVLGVVSA
                     VSALATGLTVWHGNLDLFHAARIPLFMVSLVCGLLACYQVIRGTALGKPGAAVLLAGV
                     LILTAAGVHDALLAMGAITSVARIFSGMLVYTCTLVYVVSTAIANTFVENQGLAKEPN
                     QEYLRAQWLALPATKLDIKPVSSGTQPTLALPHARHCTPNALAFTRWRASGYQAKALQ
                     PAPPRTHAALPNHSRAF"
     gene            complement(1535..2701)
                     /locus_tag="PLB25_17155"
     CDS             complement(1535..2701)
                     /locus_tag="PLB25_17155"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE43338.1"
                     /translation="MFPSVEDRVVRPGPEPVSARAKRLLSHLILSNSPKQRLLLIRSL
                     TSMVVYVAAILMVEYSIYNGLIAESQQVHWLQVGMLAWMVGVYAFLRSGLNWLLPAPA
                     LTQLQIVGANAWVILGYALCPPLRGGMMMMLVLILVFGIFEQNRRGQAIINVSTLAVF
                     GAVQWYMAQHFPNDFPARIEVFHWAMMATIVPIASILGGQINQLRVKLQVQRHELLEA
                     LDRIREMAQNDELTALFNRRYMNDLLTTTARQVERSNQTFSVCIIDIDHFKKVNDTYG
                     HRIGDEVLRSFAAQASADLRPTDTLARWGGEEFLLLMSDTRAEQALARALRLHAVISE
                     LVIAEAPELRVTFSTGIAQFRAKETIDSLIERADQALYKAKKAGRNQSILAPKS"
     gene            complement(2874..3368)
                     /locus_tag="PLB25_17160"
     CDS             complement(2874..3368)
                     /locus_tag="PLB25_17160"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HOE43339.1"
                     /translation="MNTATAEMIENVTYDELSVGQSAQLERTLRADDIRAFAAVSGDT
                     NPAHLDPEYAASTLFHGVIAHGMWGAALISALLGTRFPGPGTIYMEQNLHFTKPVHIG
                     DTLTVSATVLSKDDAHHRVVLDCQVVNQNGGVVVHGNAVVMAPTTKVRRAAIAVPTIQ
                     LIDT"
     gene            3494..>3989
                     /locus_tag="PLB25_17165"
     CDS             3494..>3989
                     /locus_tag="PLB25_17165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018715319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOE43340.1"
                     /translation="MATTPFDWQDPFLLERQLSSDERAVRDAAAAYCQGQLLPRVVQA
                     FRSGATDPAIFREMGALGLLGPTIPERYGSPGLNYVQYGLIAREVERVDSGYRSMMSV
                     QSSLVMVPIFEFGTEELRQKYLPKLASGESIGCFGLTEPDHGSDPGSMASRAHQVDGG
                     YRLTG"
BASE COUNT          868 a         1128 c         1200 g          793 t
ORIGIN      
        1 ctggccaact caaaccaggt ctcaaaatgc tcggctatgg tttggtagag cagcgtgcgt
       61 tcggggtggc gcgggttgta gagctttggc ttggtacccg cttgcccgcc agcgtgtgaa
      121 tgcgagtgcg ttgggcgtgc agtggcgggc atggggcagt gccagcgtgg gctgggtgcc
      181 tgatgatact ggttttatat ccagcttcgt cgcaggcaga gccaaccact gggcgcgcaa
      241 atactcctga tttggctctt tggccaagcc ctggttctcg acaaaagtgt tggcaatggc
      301 ggtgctgacc acatagacca gggtgcaggt gtacaccagc atgccgctga agatgcgtgc
      361 caccgaggta atggcgccca tggctaacag tgcgtcgtgc acgcctgcag cagtcaaaat
      421 cagcacgccg gccagcaaca cagccgcgcc cggtttgcct aaggcagtgc cacggatcac
      481 ttggtagcaa gctagcaggc cacacaccag agacaccata aagagcggaa tacgcgcggc
      541 gtggaacagg tccaggttgc cgtgccatac ggtcagtccg gtggcgagcg cgctgacagc
      601 gctgaccacg cccagaaccc aattaagccg gggcgtaaac cggtaataaa actggcagaa
      661 cacgtaggcc aaaaaatagg aaaaaatacc aaagtagtgc acaaaaagca gtggctctgg
      721 cgcaaaaaag cgcagcaggt agtcggcagg cgctgcataa aacgggaagc tggccaaact
      781 gccaagcgcc gcaatcaaat acatgcgcga gcgggttttg gcatacacca acaacaaaaa
      841 cagcccaaag aaaaacacca cgtaggccac gatggtgcgc aactcgccgc cccaaaactg
      901 ctcccaggcc aagcccctgt cgtgcgcgtc gtacttgcgc agttcaaagg gcagttggta
      961 aatgccggtc atcaaaggcg tatcgacccg gatggtcacg acctgctgtg cttgcttgac
     1021 gataaagcgc acgggaatcg gttggatcga gtagtagcgc tccacgttga tgttgtgggg
     1081 gcgcttgtaa acttcttgcc cgtcgatata gacgtttacc cgccccatgt aggcgtctac
     1141 cagcagcacg acttcttggc cgatcaggtc gggcgcgaat tcaaaagtac cgcggtacca
     1201 gcctacggta aagtttttgt tatcggggta cacattgcgc cacggtccgg gcgcctgcac
     1261 cagttgccag ttttgggtgt ctagcgcaac gtctttgttg tgggggttgt cgttgcgggt
     1321 aaacaaccag ttgcccgcta agggcagtgg gtgtgccagc gagtgcacca ccatgcggca
     1381 ggagttgcag tctgcccgca ggtcttgggc cgcagcaggg ctcacgacac aaagcgccgc
     1441 tagcgccagt gctagccctc tgagaagacg aacgaataag ccctgtctag cagttaaaag
     1501 cgccatgcaa aacgttgcaa agatcacagt ttgtttatga cttaggggca agaattgact
     1561 ggttgcgccc agcctttttg gctttgtaca gggcctggtc agcgcgctcg atcagcgagt
     1621 ctatggtctc cttggcacga aactgtgcaa ttcccgtgga aaaagtgacg cgcaattccg
     1681 gcgcctcggc aatcaccagt tcgctgatga cggcatgcag gcgcagcgcg cgggcaaggg
     1741 cttgctcagc acgggtgtcg ctcatgagca gcaagaattc ttcaccaccc catcgggcca
     1801 gagtgtcggt gggccgaagg tcggcactgg cctgcgcggc aaaactgcgc agcacctcgt
     1861 caccaatacg gtggccgtag gtgtcattga cctttttaaa gtggtcaata tcgatgatgc
     1921 acacgctaaa tgtctggttt gagcgctcca cttgccgcgc ggtggtggtc aacagatcgt
     1981 tcatgtaacg gcggttaaac agcgcggtca attcatcgtt ctgcgccatt tctcggatac
     2041 ggtccaatgc ttcgagcagt tcgtggcgtt gtacctgcag ctttacacgc agctggttta
     2101 tttgtccgcc caaaatcgac gcgatcggca caatagtggc catcattgcc caatggaaaa
     2161 cctcaatgcg tgccggaaaa tcattcggga agtgttgcgc catgtaccac tgcactgcgc
     2221 caaacacggc cagtgtcgat acgttgataa tggcttggcc ccggcggttt tgctcaaaaa
     2281 tgccaaatac caaaatcagc acgagcatca tcatcatccc gccccgcagc ggtggacaca
     2341 gggcgtaacc cagaatgacc caggcattgg cgcccacaat ctgtaactgc gttagcgcgg
     2401 gagcaggcaa cagccaattg agcccgctgc gcaaaaaagc gtacacgcca accatccagg
     2461 ccagcatgcc aacttgcagc caatgcacct gctgggattc cgcaatgagc ccgttgtaga
     2521 tggagtactc aaccatcagg atcgcggcca cgtagaccac catcgaggtg agcgaccgaa
     2581 tcaggagcag ccgttgtttg ggcgagttgc tcagaatcag atgtgagagc aagcgcttgg
     2641 cgcgcgcgga cacgggctcc gggcccggac gcaccactct atcttcgact gacggaaaca
     2701 cgctattgcc cagcatacac tcccacgaaa cgacagccac aagttccaat gtatcacgct
     2761 attaaccaac gacctatccc gcgcgtgctg cgtaattgcg ccttgcgaaa gcccgcaatt
     2821 gccgtgctga tactctcaga aataaggcgc agggtagcgc cgtggcggtc tattcaggta
     2881 tcgatcagtt ggatggtggg caccgcgatg gcggctcggc gcaccttggt tgtgggcgcc
     2941 atgacgacgg cgttgccgtg caccaccacc cccccgtttt ggttgaccac ctggcaatcg
     3001 agcaccaccc ggtggtgcgc atcgtctttg gacagcactg tggccgaaac ggtgagcgtg
     3061 tcgccaatat gcaccggttt ggtgaagtgc aggttttgct ccatgtaaat ggtgcctggc
     3121 cctggaaacc gcgttcccaa caaggcggat atcagtgcgg cgccccacat gccgtgggcg
     3181 atgacgccat gaaacagtgt gctggcggcg tactcggggt ccaggtgggc tgggttggtg
     3241 tcacccgaca cggcggcaaa tgcgcggatg tcgtcggcgc gcaaggtgcg ttccaactgg
     3301 gcgctttggc cgacggagag ttcgtcgtag gtgacgtttt cgatcatttc agcggtggca
     3361 gtgttcatgg gctcgctcta cttcgatagg gtttatgctg gcagggtcat ggtctggtgg
     3421 gtgacacgtt tttcaggcta acacccccca cggccgttag gattgagcct gatcaagttt
     3481 gcggagattc cacatggcca ccacaccgtt tgattggcaa gaccctttct tgctggagcg
     3541 ccagctcagc agcgacgagc gcgcagtgcg cgacgcggca gcggcctatt gccagggcca
     3601 gctgttaccg cgggtggtgc aggcgtttcg cagtggggca accgacccgg ccatctttcg
     3661 ggagatgggt gccttggggc tcttgggccc caccatcccc gagcgctacg gcagcccggg
     3721 tctgaactat gtgcaatacg ggctgatcgc ccgcgaggtc gagcgggtgg acagtggcta
     3781 ccgctcgatg atgagtgtgc aaagctccct ggtgatggtg ccgatctttg aatttggcac
     3841 cgaagaactg cgccagaaat acctaccaaa gttggccagc ggcgagtcga ttggctgctt
     3901 tggcctgacc gaacctgacc atggctctga ccccggctcc atggccagcc gtgcccacca
     3961 agtggatggc ggctaccggc tgaccggca
//
LOCUS       DAOSLL010000470         3983 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19982_length_3983_cov_3.367617, whole genome shotgun sequence.
ACCESSION   DAOSLL010000470 DAOSLL010000000
VERSION     DAOSLL010000470.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19982_length_3983_cov_3.367617"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(3..950)
                     /locus_tag="PLB25_17170"
     CDS             complement(3..950)
                     /locus_tag="PLB25_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43341.1"
                     /translation="MNRKFFSVIACLASVCVLGACSFASQQLSDKRMEEQLLRPGALD
                     SPVATNFDEYHFKFLDKYRIVHAWPVTSACKYAGRRTMYKMGETSYGEVQWVLDIIEE
                     GRSTKQRDADPGWNFNRFVRSVKVQRPVYDTVNGVRGIVRYEENEEGLQPICFEAWVG
                     SSHAVTIFLYQRTVDQWQDALAKWSKGAYLGKDAVQGIDTVMGNKWHVYRLPLKPREM
                     NRVAGAYELRILPIGDTGYTFAMEFGATVESLENPQAHAAFQAMFNRLIESVKIEPLT
                     PAIEADMAQLKAKAFEIVRQNCLRMKNPPGYCKKYLEPQ"
     gene            complement(959..2494)
                     /locus_tag="PLB25_17175"
     CDS             complement(959..2494)
                     /locus_tag="PLB25_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43342.1"
                     /translation="METLQDKSTYSESSSSSGAGVSVCVPPLCYGTSSANISASKTDI
                     NSNYQSANEQTGIKAGDGGFQVSVRGNTDLNGGVMASSDKAITGNKNSLSTGTLTTSD
                     LNNEASASAATSGFSLSTDMLSQGKYGAAKGVLTNIMDNASQSGSSSGQTKAAISAGT
                     VRITDDAAQTQLTGNTGAQTIASLNRDTQNANTVAQRQDVQAMKQTVEAERAIKNEAV
                     KQLTTLTDEAYRVMFKEVPKFYKVTCPANTNCTTNPEKTTVQLVDKDTQTELANAPSG
                     AVLAVNGIDNPLERAGQLAMQNAELVRDANGVEAKPTTIYLMHYVPANNGLSELMIAA
                     YEKSLAPTLGYSNQDEAYAAAIQARGQLETTSLGHSRGTIVQTNANNILGEQKFTNPN
                     LSVRGVGGAVTGETYTDAALKVAGVDAARNITFTYFKNDPVPVVAGGNSGVISLSEFW
                     KVLTTSNSTHSCYGTGAAGCAQVEILTPSAPKDANQKNSSLIRYIGGKPFDASGKPLE
                     LNK"
     gene            complement(2431..>3983)
                     /locus_tag="PLB25_17180"
     CDS             complement(2431..>3983)
                     /locus_tag="PLB25_17180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015867291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HOE43343.1"
                     /translation="ALGGMYAGKITLVGTEVGVGVSNAGSIAANGGALVVQANGMLTN
                     TGLIEGQTALIKATTVNNTGTGQIYGDTVAIQADTLNNAPAVVGQSAPVIAARTGRMD
                     LGVEALNNQEGAIIYSQGDLAIGGSLDANNLATGRAGTITNNSATIESLGSMDIKTQN
                     LTNSYTSLTYRVETVSALPGSSSCGGDCYSNYNETTYRAVAVDGVNGAGKILASNNIT
                     LDASNNALNSSGQILAGGALNVTGTAVNNQGIDVALFKEQRGTTYQRRTDRYWDWGWK
                     ERAWWEASAYSNDVADNQTVQRGESPPTSPTARSAFSPNASTVGNLPTSAQYRINNNL
                     GAAYLVETNPRFANMRNWLGSDYMLASLVLDPSVTQKRLGDGFYEQRLINEQVAQLTG
                     RRYLGNYTSDEAQYQALMNGAITYAQTFNLRPGIALTAEQVAQLTSDIVWLVEKEVTL
                     KDGRKQRALVPQVYAMVREGDLSTTGALLSGNTLNISTTGDVRICPWKPCRTKAPTAK
                     AVAAAARG"
BASE COUNT          741 a         1082 c         1095 g         1065 t
ORIGIN      
        1 agtcattggg gttcgagata cttcttgcaa tacccaggtg gattcttcat gcgcaagcaa
       61 ttctggcgga cgatttcaaa tgctttggct ttgagttgtg ccatatctgc ttcaatggct
      121 ggagtcaggg gttcgatttt gactgactca atcagacgat tgaacatggc ctggaaggcg
      181 gcgtgtgctt gggggttttc tagtgactcc acagtggcac caaattccat cgcaaaggtg
      241 tatccggtat cccctatggg caatatgcgc agttcatagg ctcccgcaac gcggttcatc
      301 tcgcgtggtt ttagtggcaa tcggtagaca tgccacttgt tacccatgac agtatcaata
      361 ccttgcactg cgtccttgcc aaggtatgcg cctttgctcc atttcgccag tgcatcttgc
      421 cactgatcaa cagtacgttg atatagaaaa attgtcacgg catgactact gcccacccaa
      481 gcctcaaagc aaatcggctg caagccttct tcgttctcct cgtaacgaac aataccgcgc
      541 acgccattca ccgtgtcata gacaggccgc tgcactttga cgctgcgaac gaagcggttg
      601 aagttccatc ccggatctgc atctctttgc tttgtgctcc taccttcttc gatgatgtca
      661 agcacccact ggacttcgcc atacgacgtc tcccccattt tgtacatcgt cctacggcca
      721 gcatatttgc aagccgaagt caccggccat gcatgaacaa tcctgtactt atccagaaat
      781 ttaaagtggt attcatcaaa gttggttgca acgggcgagt ccagtgcacc tggcctcaag
      841 agttgttctt ccattcgctt gtcagaaagc tgctgagatg cgaacgaaca agcaccaagt
      901 acgcacacac ttgcaaggca agctatcaca gagaaaaatt tgcgattcat acaaaacctc
      961 acttattcag ttctagtggt ttgccactgg catcaaaagg cttaccaccg atgtacctaa
     1021 tcaagctgct atttttttgg tttgcgtctt tgggggcgct gggagttagt atttccactt
     1081 gggcgcaacc agcagccccg gttccgtagc aactgtgcgt gctgttggat gttgtcagaa
     1141 ctttccaaaa ctccgacaaa ctaatcactc cagagttgcc gccagcaact acagggaccg
     1201 ggtcattttt gaagtaggta aaggtgatat ttctcgccgc atcaacgccg gcaaccttaa
     1261 gtgctgcatc agtgtaagtc tccccagtca ccgcaccgcc caccccgcgc acactcaagt
     1321 tcgggttggt gaatttctgc tcgccaagaa tgttgttggc attcgtttgc acgatggtgc
     1381 cccgactgtg ccccaggctg gtggtctcta gctgcccccg tgcctggatg gcggccgcat
     1441 aggcttcatc ctggttggag tagcccaacg tgggcgcaag gcttttttca taggctgcga
     1501 tcatgagttc actcaatccg ttgttggcgg gtacgtagtg catgaggtag atcgttgtgg
     1561 gcttagcttc aacgccattt gcatctctaa caagctccgc attctgcatc gccagttgcc
     1621 cagcgcgctc cagtgggttg tcgatgccgt tcacagccaa cacagccccg ctaggtgcat
     1681 tggccagttc agtttgggta tctttgtcca ccagttgtac ggttgttttt tcgggatttg
     1741 tggtgcagtt tgtattggca ggacacgtta ccttgtaaaa cttgggtacc tctttgaaca
     1801 tgacccggta ggcttcatcg gtcagcgtgg tgagttgctt gaccgcttca ttcttgatcg
     1861 ctcgctcggc ctccacagtc tgtttcatgg cctgtacatc ctggcgctgc gccaccgtgt
     1921 ttgcattttg ggtgtcgcga ttcagactgg cgatggtctg cgctccggtg ttgccagtca
     1981 gctgggtttg cgcggcatca tcggtgatgc gcacagtgcc tgcgctgatg gctgctttgg
     2041 tctgaccgct gctactgccc gattgactgg cgttgtccat gatattggtc agcacaccct
     2101 tggcagcacc atatttgcct tgagacaaca tatccgtact taggctgaaa ccgctggtcg
     2161 cagccgatgc gctggcttca ttgttcaaat cactcgtagt gagcgtgccg gtactaaggc
     2221 tgttcttgtt gcccgttatc gctttgtcac tgctggccat cacgccgccg ttgaggtcgg
     2281 tattgccccg cacgctgacc tgaaagccgc catcacctgc cttgatgccc gtttgttcgt
     2341 tggcgctttg gtagttgctg ttgatgtcgg ttttactggc gcttatattt gcgctgctgg
     2401 tgccgtagca caggggggga acgcacacgc tcacccccgc gccgctgctg ctactgcttt
     2461 cgctgtaggt gcttttgtcc tgcagggttt ccatggacag attctcacgt cacccgtggt
     2521 gctgatgttc aacgtgttgc cagagagcag ggcgccagtg gtcgacaagt caccttctcg
     2581 caccatggcg tagacctgag gcaccagggc gcgctgcttg cggccgtctt tgagggtgac
     2641 ttctttttcg accagccaga cgatgtcgct ggtgagttgg gctacttgct ctgcggtcag
     2701 ggcaatgccg ggacgcaggt tgaaggtctg ggcgtaggta atagcgccgt tcatcagcgc
     2761 ctggtattgg gcttcgtcgc tggtgtagtt gcccaaatat cggcggccag tgagttgggc
     2821 cacttgctcg ttaatcaggc gttgctcata gaacccgtcg cccaaacgtt tttgggtcac
     2881 gctggggtct aggaccaggc tggcgagcat gtagtcgctg cccagccagt tgcgcatatt
     2941 ggcgaagcgt ggatttgtct ctacgagata agcagcaccc aggttgttgt tgatgcggta
     3001 ttgcgctgac gttggcaggt ttcctacggt actggcattg ggactgaatg cgctgcgtgc
     3061 cgttggtgag gttgggggag attcaccgcg ctggaccgtt tggttgtcgg ctacatcgtt
     3121 gctgtaggcg ctggcctccc accatgcacg ctctttccaa ccccagtccc agtaacgatc
     3181 ggtccgccgt tggtaggtgg tgcctcgctg ttctttgaac agggcgacgt cgatgccttg
     3241 gttgttgact gctgtgccag taacgttcaa agcaccgccg gccagaattt gaccactgct
     3301 attgagcgcg ttgttgcttg cgtccagcgt gatgttattg gaggccagaa tctttccagc
     3361 gccattcacg ccatctaccg ccaccgcacg gtaggttgtt tcgttgtaat tcgagtaaca
     3421 gtcaccgccg cagctgctgc taccaggcag tgcagaaaca gtttctaccc gataggtcag
     3481 cgatgtgtag ctgttggtca ggttctgggt tttgatgtcc atcgagccca gggattcgat
     3541 ggtggcgctg ttgttggtaa tagtgccggc tcgaccagtt gccagattgt tggcatccag
     3601 actgccgcca attgccagat ctccttggct atagatgatt gcaccttctt ggttgttcag
     3661 cgcctccaca cccaagtcca tacggccagt ccgggcggca atgacgggag cgctctggcc
     3721 aaccactgcg ggggcgttgt tcaaggtgtc tgcctggatg gcaaccgtat cgccgtagat
     3781 ttgtcctgtg ccggtgttgt tgaccgtggt ggctttaatg agagcggttt gaccttcgat
     3841 caagcctgta ttggtcagca tgccgttggc ttgcactacc agggctccac cattggccgc
     3901 aatggaccca gcattgctga cccccacgcc gacctcggtg cctaccaggg tgatcttgcc
     3961 ggcatacatg ccgccgaggg ctg
//
LOCUS       DAOSLL010000471         3981 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_19995_length_3981_cov_4.394040, whole genome shotgun sequence.
ACCESSION   DAOSLL010000471 DAOSLL010000000
VERSION     DAOSLL010000471.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19995_length_3981_cov_4.394040"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1288
                     /locus_tag="PLB25_17185"
     CDS             <1..1288
                     /locus_tag="PLB25_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HOE43344.1"
                     /translation="WSASGLEVVPGDFSEVLANDACPAAPNVILANPPYVRHHHICRD
                     DKERLQALALKMTGARVSGLAGLYVYFLLLATAWLQEGGIAAWLIPSEFMDVNYGTVL
                     KDFLGRRVTLIRVHRFSPSDAQFGDALVSSVVLVFRKLPPKPDHSVEFTFGGTLAKPD
                     AADSIGLELLRATRKWTMFPDTVTNDRHALNDGNSPALGDFFRVQRGIATGCNAFFVL
                     KRAQAISLGLPSQFLRPILPSPRHLKTTCIDAGSDGYPLLDSPLCVIDCNLPENLIET
                     QYPALWSYLKTAQSRGVMEGYLIGKRSPWYRQEQREPSAFLCTYMGRGSGDKAPFRFI
                     WNRSRAIGSNLYLMLYPQNGLADMLCQHPNRAQLVHTLLSEVTGDELRGEGRIYGGGL
                     NKIEPSELARISAASFVRLWPEIGARAPMQQAELFG"
     gene            1352..2614
                     /locus_tag="PLB25_17190"
     CDS             1352..2614
                     /locus_tag="PLB25_17190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HOE43345.1"
                     /translation="MTARTQTEISTLRINGQRLWGSLMELAQIGATAKGGVCRLTLTD
                     LDKQGRDLVTQWGREAGLAVTIDKIGNGFMRRAGRNNALPPIVMGSHIDTQPTGGKFD
                     GNYGVLSGLEVMRTLNDHGIETEAPVEVSFWTNEEGSRFVPVMMGSGVFARAFTLAHA
                     YAARDTEGKSVGDELARIGYIGEQEPGEHPIGAYFEAHIEQGPVLEDNDVTIGVVQGV
                     LGIRWFDCTVTGMEAHAGPTPMALRRDAMQVATHIMQEVVACALRHAPHGRGTVGMVQ
                     VFPNSRNVIPGRVKFSIDLRNATDALVDLMADEVKAFAASLGSKTGLGIQIDLVSSYP
                     AQAFHEDCKGAVARAAAKLGYSNMPAVSGAGHDAVYMAKLAPSGMIFIPCLNGISHNE
                     IESAKPEHITAGCNVLLHAMLERAGVVG"
     gene            2795..3796
                     /locus_tag="PLB25_17195"
     CDS             2795..3796
                     /locus_tag="PLB25_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010108380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HOE43346.1"
                     /translation="MSLKPFTRRQAIVLATTLVALTPGLTLAQAKLKVAGIYTVPFEQ
                     QWAGRLHQALKAAEARGEIEYKASENVSNADYERVMREYATAGNQLIVGEAFAVEAAA
                     RKVAKDFPKVSFLMGSSGKPVAPNFSVFDNYIQEPAYLSGMVAGSMTKSNKIGMVGGF
                     PIPEVNRLMNAFMAGAKETNPKVEFSVSFINSWFDPPKAKEAAFAMIDKGADVMYAER
                     FGVSDAAKEKGKLAIGNVINTQAQYPDTVVASALWHFEPSADRAIKLALAGKFVAEDY
                     GPYSMMKHKGSSLAPLGTFEKKVPADIIAKVKAKEADIVAGKFNVKVDDSQPKSTAK"
BASE COUNT          833 a         1182 c         1190 g          776 t
ORIGIN      
        1 gtggtctgca tcagggcttg aggttgtgcc aggtgacttt agcgaggtgc ttgctaacga
       61 cgcatgcccc gctgcaccaa atgtaatcct ggccaacccg ccctatgttc ggcaccacca
      121 tatatgccgg gacgacaagg agcgcctgca ggcactcgcc ctcaagatga ccggtgcaag
      181 ggtgagtgga ctggcgggtc tgtatgtcta ttttctattg cttgccactg catggctgca
      241 agagggagga attgccgctt ggctgattcc ctcggaattc atggatgtca attacggcac
      301 cgtgctcaag gattttctgg gtcgtcgggt aacgctgatc cgggtccatc gtttcagccc
      361 gagcgatgcc cagtttggcg acgcgctggt gtcctcggtt gtgctggtct ttcgcaaatt
      421 gccgcccaag ccggaccaca gcgtcgaatt taccttcggg ggaacactgg ccaaaccaga
      481 cgctgccgac tccatcgggc tggaactgct gcgtgctacg cgcaagtgga cgatgtttcc
      541 agacaccgtg acgaatgaca gacacgcgct aaacgatggc aatagtccgg cgctgggtga
      601 cttctttcgc gttcaacgtg gcattgcgac cggctgcaat gcgtttttcg tgctcaaacg
      661 tgcgcaggcc atcagcctcg gcttgccgtc ccaatttctt cgcccgattc ttcccagccc
      721 gcgtcacctt aaaaccacct gcatcgacgc aggcagtgac ggctacccgc tgctcgacag
      781 tccgctgtgc gtcattgact gcaacctgcc ggaaaacctg atcgaaacac agtacccggc
      841 cctgtggtcg tacctcaaga cagcccagtc gcgcggtgtc atggagggtt atttgatcgg
      901 caagcgcagc ccctggtatc ggcaggaaca acgcgagcca agcgcctttc tatgcaccta
      961 catgggtcgc ggcagcggcg acaaagcgcc ttttcgtttt atctggaacc gatccagggc
     1021 gattggcagc aacctgtatc tgatgcttta cccgcaaaac ggtctggctg acatgttgtg
     1081 tcagcatccc aaccgcgcac aattggtaca cactttgctc agcgaggtga ccggcgatga
     1141 actgcggggc gaaggtcgaa tttacggagg tggcttgaac aagattgaac ccagtgaact
     1201 ggcgcgtatt tcagccgcct catttgtccg gctttggccg gaaattgggg ccagggcgcc
     1261 gatgcagcaa gccgaactgt ttggctaaac cgacctgccc tgtcaaaaga acatcgttat
     1321 ctggcacata cctttttttg gagaattgca tatgactgct cgaacccaaa ccgaaatcag
     1381 caccctgcgc atcaacggcc agcgcctgtg gggcagcctc atggaactgg cccagattgg
     1441 tgccaccgcg aaaggcgggg tttgccgcct gaccctgacc gacctggata aacagggacg
     1501 cgacctggtc acccagtggg gtcgcgaggc tggccttgcc gttaccatcg acaagatcgg
     1561 caatggcttc atgcgccgcg ctggccgcaa caacgccctg ccaccgatcg tcatgggcag
     1621 ccacatcgac acccagccga ccggcggcaa gttcgacggc aactacggcg tgctgtctgg
     1681 cctggaagtg atgcgcaccc tcaacgatca tggcatcgag accgaagcgc cggtcgaggt
     1741 gagcttctgg accaacgaag agggttcgcg ctttgtgccg gtgatgatgg gatcgggcgt
     1801 gtttgccagg gcgttcacgc tggcgcacgc ctacgcagca cgcgacaccg agggcaaatc
     1861 ggtcggtgac gaactggccc gcatcggcta catcggagag caagagccgg gcgagcatcc
     1921 gattggcgcg tattttgagg cgcacattga acaaggcccg gtgctcgaag acaacgacgt
     1981 gacgattgga gtggtacagg gcgtgctggg catccgctgg tttgactgca ccgtcaccgg
     2041 catggaagcg cacgccggtc caactccgat ggccttgcgc cgcgacgcga tgcaggttgc
     2101 cacccacatc atgcaggaag tggtggcttg cgcgctgcgc cacgcgccgc atgggcgcgg
     2161 cacggtcggc atggtgcagg tgtttcccaa cagccgcaac gtgattccgg gccgggtcaa
     2221 gttcagcatt gatctgcgca atgccaccga tgcactggtg gacctgatgg ccgacgaggt
     2281 gaaggccttt gccgcaagcc ttggcagcaa aaccggcctg ggcatccaga ttgacctggt
     2341 gtcaagctac ccggcgcaag cctttcatga ggactgcaag ggtgcggtgg cgcgcgccgc
     2401 tgccaagctg ggctactcca acatgccggc agtgtcgggg gccggacatg atgcggtcta
     2461 tatggccaaa ctggccccga gcggcatgat cttcatcccc tgcctgaacg gcatcagcca
     2521 caacgagatc gaatcagcca agcccgagca catcaccgct ggctgcaatg tgctgctgca
     2581 cgccatgctg gagcgcgccg gggtggtcgg ctgacatcaa agccggacaa aaccctaatg
     2641 cccgacgcaa ttgtgatggc ttgcctgagg cgccggctgg ccgagcctgg cacgtaaagt
     2701 gcttgctgga tttcttgcaa aaattgcccc ccagcccttg tgcaatctgc gcagccagct
     2761 atatattttg tagtgtttta agaaaggaaa aaccatgtcg ttgaaaccgt tcacccgtcg
     2821 ccaggcgatt gtgctggcca ccaccctggt cgctttgacc cccggcctga ccctggcgca
     2881 agccaagctc aaggtcgctg gcatctacac cgtgccgttt gagcagcaat gggctggtcg
     2941 gctacaccag gcgctaaagg ctgccgaagc gcgtggcgag atcgaataca aggccagcga
     3001 gaacgtcagc aatgccgact acgaacgtgt catgcgcgaa tacgccactg ccggcaatca
     3061 gttaatcgtc ggcgaagcgt ttgcggtgga agccgctgcg cgcaaggtgg ccaaggattt
     3121 tcccaaggtg tcgttcctga tgggttccag tggcaaaccg gtcgccccca atttcagcgt
     3181 gtttgacaac tacatccagg agcccgccta cttgagcggc atggtggctg gcagcatgac
     3241 caagagcaac aagatcggca tggtcggcgg cttcccgatt cccgaagtca accggctgat
     3301 gaacgccttc atggccggtg ccaaagaaac caaccccaag gtcgaattca gcgtcagctt
     3361 catcaacagt tggtttgacc cccccaaagc caaggaagcc gcttttgcca tgatcgacaa
     3421 aggggccgac gtgatgtatg ccgagcgatt tggcgtgtct gacgcagcca aggaaaaagg
     3481 caagctggcg attggcaacg tcatcaacac gcaggcgcag taccccgata ccgtggtggc
     3541 atcggccctg tggcactttg agccgtcggc cgaccgcgcc atcaagctgg cactggcggg
     3601 caaatttgtc gctgaagact acggccccta ttccatgatg aagcacaaag gttcgtcgct
     3661 ggcaccgctg ggcacattcg agaagaaggt gccagccgac atcatcgcca aggtcaaggc
     3721 caaggaggcc gacatcgtcg ctggcaagtt caatgtaaag gtcgatgaca gccagcccaa
     3781 gtcaacggcc aagtgaacct gaccaaacaa cccggcttgt ttgcagcgca cgctcctgtt
     3841 gcctgttgcc tgttgcccgt tctgcttgtg agccagtgag gcggtgatgg ccaccgaatc
     3901 gaaagtccgg ttgatgccgg tgctggccgc cagatgcatg ctgcgtttgc cgggtgaaat
     3961 caagccgcgc gccgggattg c
//
LOCUS       DAOSLL010000472         3978 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_20013_length_3978_cov_4.071884, whole genome shotgun sequence.
ACCESSION   DAOSLL010000472 DAOSLL010000000
VERSION     DAOSLL010000472.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3978
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20013_length_3978_cov_4.071884"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(209..727)
                     /gene="mobB"
                     /locus_tag="PLB25_17200"
     CDS             complement(209..727)
                     /gene="mobB"
                     /locus_tag="PLB25_17200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HOE43347.1"
                     /translation="MKVFGITGHSGMGKTTLLERLVPEISSRGLVVSLIKHSHKNIDI
                     DRPGKDSYRLRESGCKEVLLLGNDRWALMHELRGASEPSLDYLMDRMQACDLLLVEGF
                     TPGQFPKLEVWRSSLGVLTLWPDWPGIVAIASEKPPRSAAQMAVTPGLSHLDLADTKS
                     IAGFVLLHASER"
     gene            complement(811..1131)
                     /locus_tag="PLB25_17205"
     CDS             complement(811..1131)
                     /locus_tag="PLB25_17205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HOE43348.1"
                     /translation="MTGTLLWVVCAIAPAHATVDVEAAKALAKANDCLKCHAADKDKR
                     GPSMGKIAAKYKGVPDARAKAVEHMTTGPLVKLSDGSEINHKIVDSKDVNGLANIADW
                     FLSH"
     gene            complement(1233..1577)
                     /locus_tag="PLB25_17210"
     CDS             complement(1233..1577)
                     /locus_tag="PLB25_17210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOE43349.1"
                     /translation="MKAKSLSILSGTFLLALSAMAPAQAAVDVEAAKALAKANDCLKC
                     HAADKDKKGPSMKKIAAKYKGKPDGQEKAIKNMTEGQMVKLTDGTEEKHKVIDTKDPK
                     ELKNMADWYLSH"
     gene            complement(1670..2299)
                     /locus_tag="PLB25_17215"
     CDS             complement(1670..2299)
                     /locus_tag="PLB25_17215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HOE43350.1"
                     /translation="MEKVYIHPLPVRIWHWVNALGFLMLIATGVQIRYLDLVQLIPFR
                     TAVVVHNYIGFVLIANFFLWLCFYLFTDKITVYLPEMDPKKYFEDAWRQIKFYGHGIF
                     LGEDNPHQATIYHKFNPLQIMMYQIIMLLGLPIMFGSGLLLWDIKRFSAVIDFFGGVR
                     VVDTVHVLMFIGFSGFLLMHLYLITLGPTRFAHFKAMLTGFEERHGNSK"
     gene            complement(2302..3750)
                     /locus_tag="PLB25_17220"
     CDS             complement(2302..3750)
                     /locus_tag="PLB25_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43351.1"
                     /translation="MNRTSKTSHRWLRRGILLSGLLGLAFAFLEPGAVAADAASAVPA
                     AQTTSAPKTISAADKPRDDATLGDEDQACQKCHDKSDIPAKKTEAGEMLSMRIDTKEF
                     LQSMHFETSCTDCHDSADGKDHGSVAVPMKSKRDYRLSFQNACTTCHKKNVAEFKDSL
                     HAVLVKEGSDKAPTCSDCHNSHTLRSVKLVEPIANVPCANCHKDIFRAYSEDVHGLER
                     VAKGKKAPLCADCHSSHKILAASLGDGIKEACLACHKDSITKHEVWLPNAGQHFEAIS
                     CAVCHAPYAQRRVNLRMVDSVGGQQILEKKGVPRFDRLTKAADGSDFGLDAKELRSFL
                     TEFNLENPGSKAILSGRLEVRSGLQSHQLSEKEQALKDCKVCHQAGAVPFVSVVLSIA
                     GPDGRPLRQGVEKEVLTSAAAFGSMRGFYAIGSTRIKLLDYLLVLVLLGVLCVPIAHL
                     TAHRLFKRKRDERNATRLKAAQVTTMQQKSKD"
BASE COUNT          909 a         1120 c         1037 g          912 t
ORIGIN      
        1 ctgtcagcgc tttgctgaat agtgccattt tctcggggga agttgcactt ttgctgtgtg
       61 gttcggcgca aactatcggc caactgaatc caggtttgtt gcagccactg tctgattgac
      121 tgcccaccga cccaccatgg cctcggcgtt gtcactgtgc tggttcacac tggccgaaac
      181 aacaccgatc tgtcatggtt gctacttgct aacgctcact tgcatgcagc aagacaaacc
      241 ctgcaatcga tttggtatcg gccagatcga gatgcgacaa acctggggtc actgccatct
      301 gtgccgcaga cctgggcggt ttttcgctgg caattgccac aatgcctggc caatccggcc
      361 acaaggtcag tacacccaaa gaagaccgcc acacttcaag cttgggaaac tggccgggcg
      421 tgaaaccttc aaccagcagc agatcacacg cttgcatgcg gtccatcagg tagtcaagag
      481 atggctcact ggcaccccgc aattcatgca tcaaggccca gcgatcattg ccgagcagca
      541 atacctcttt gcaaccggat tcgcgcaagc gataagagtc cttgccaggg cggtcaatgt
      601 caatattctt gtgactatgc ttgatcagcg agaccaccaa gccccgtgac gaaatttcag
      661 gaacaagacg ctccaacaag gttgtcttgc ccatcccgga atggccggtg ataccgaaca
      721 ccttcatgac tattgcctca ccagaacgaa ctcaaacaag taacatgaaa caaatggcaa
      781 ttcacagcgc aaagcaccag cttgcgaatg tcagtgggat agaaaccagt ctgcgatatt
      841 cgccagccca ttgacatcct tgctgtcgac gatcttgtga ttaatttcgc tgccatctga
      901 tagtttgacc aaggggccag tcgtcatgtg ttcaacagcc ttcgcccgag cgtcaggcac
      961 acctttgtac ttggcggcta tctttcccat cgacgggcct cgtttatcct tgtcagccgc
     1021 atggcacttc aggcaatcgt tggccttggc cagcgccttg gcggcctcga catccacagt
     1081 ggcatgtgcc ggtgcaatag cgcatacaac ccataagagc gtgccggtca aaatagaaaa
     1141 ccgtgcattt gccacgaaac ccccgtcaaa ttcatatcag caacaccacc aaaccgtgtg
     1201 atgagatttc tgatcccggc cgcttcagtc gatcagtggg acagatacca gtcagccatg
     1261 tttttcagct ctttgggatc ttttgtgtca atgaccttgt gtttttcttc ggttccgtct
     1321 gtgagtttga ccatttggcc ttcggtcatg ttcttgatgg ccttctcttg accatcgggc
     1381 ttgcctttgt atttggcagc aatctttttc atcgacgggc cttttttgtc cttgtcggct
     1441 gcatggcact tcaggcagtc attggccttg gccaatgctt tggcggcttc aacatcaaca
     1501 gcggcctgcg cgggtgccat ggcactcagt gccagcaaga aggtgccgct caaaatggac
     1561 agggatttag ctttcatgtg gttttccttt aacgggaggt tgcaataaga cggagcatat
     1621 cgtggcaaaa attggctgct ggtgaacggc ttggtgatca tcacagcagt catttcgagt
     1681 tgccgtgcct ttcctcaaac cctgtcaaca tcgccttgaa gtgtgcaaag cgggtcggac
     1741 ccagggtgat cagatacaag tgcatcagca ggaatccgct gaagccaata aacatcagca
     1801 catgcacggt atccaccacc cgcacaccgc caaagaagtc gatcaccgcc gagaaccgct
     1861 tgatatccca caacaggagc cctgagccaa acatgatcgg caagcccagc agcatgatga
     1921 tctggtacat catgatctgt agcgggttga acttgtggta gatcgtcgct tggtgcgggt
     1981 tatcttcgcc caggaagatg ccatggccgt aaaacttgat ctgccgccag gcatcctcaa
     2041 aatacttctt tggatccatc tcgggcagat acacggtgat cttgtcagtg aacaagtaaa
     2101 agcacaacca taggaaaaaa ttagcaatca gcacaaaacc aatgtagttg tgtaccacca
     2161 ccgccgttct gaacgggatc aactgcacca gatcaaggta cctgatctgg actccggtgg
     2221 caatcagcat cagaaacccc agggcgttga cccaatgcca gatgcgcacc ggtagaggat
     2281 gaatgtagac tttttccatg gtcagtcctt acttttctgc tgcatcgttg tgacctgagc
     2341 cgccttgagc cgcgtggcgt tgcgctcatc gcgcttgcgc ttgaaaaggc ggtgtgcggt
     2401 caggtgcgct attggcacac acagcacccc cagcagcacc aacaccagca ggtaatcgag
     2461 cagcttaatg cgtgtgctgc cgatggcata gaaaccccgc atcgacccga acgcggcggc
     2521 tgaagtcagc acctctttct ccacaccctg gcgcaacggt cgaccatccg gaccggcaat
     2581 ggacagcacg acgctcacaa aaggcacagc accggcttga tggcaaacct tgcaatcctt
     2641 aagcgcttgc tctttttcgc tgagctgatg ggactgcagg ccagagcgaa cttcaaggcg
     2701 gccactgagg atggccttgc tgccggggtt ttcaaggttg aactccgtga ggaaactgcg
     2761 cagctctttt gcatcaagtc cgaaatcgct gccatcagcg gctttggtca ggcgatcaaa
     2821 tctgggaaca ccttttttct caaggatttg ctggccaccc acactgtcca ccatccgcag
     2881 gttgacccgc cgctgcgcgt agggggcatg acagactgcg cacgaaatgg cttcaaagtg
     2941 ctgccctgcg ttgggcaacc acacctcgtg cttggtgatg gagtccttgt ggcaggccag
     3001 gcaggcctcc ttgatgccat cgcccagaga agccgccaaa atcttgtgag agctatggca
     3061 atccgcacag agtggtgctt tcttgccttt ggcgacacgc tcaagcccgt gaacgtcttc
     3121 cgagtaggcc cgaaagatat ccttgtggca gttggcgcaa ggcacgttgg cgatgggctc
     3181 gaccagtttg accgagcgca gcgtatggga gttatggcaa tccgaacagg tgggggcctt
     3241 gtcgctgcct tccttgacca gcaccgcatg caagctgtcc ttgaactccg caacattctt
     3301 tttgtggcag gtggtacagg cgttctggaa agacaagcgg tagtcgcgct tgctcttcat
     3361 cggaacggca acactgccat ggtccttacc gtctgcgctg tcgtggcagt cggtacaact
     3421 ggtttcaaag tgcatcgact gcaaaaactc cttggtgtca atccgcatcg acagcatctc
     3481 gcccgcttct gtctttttgg ccggaatgtc cgacttgtca tggcattttt ggcatgcctg
     3541 gtcttcatca ccgagcgtgg catcgtcacg cggcttgtcg gcagcgctga tggttttggg
     3601 cgctgatgta gtctgagcag caggcaccgc cgaagcggca tcagccgcga ccgccccagg
     3661 ctcaagaaag gcgaatgcca ggcccagcaa acctgacagc aaaatgcctc gtctcagcca
     3721 tctgtggctg gttttggaag ttctattcat gaacaaagtc tcatgattca agggatgacc
     3781 gtttgcggtg caatccatca gtactgttgc gcgggatgca aaaaagcctc tgtcgatgga
     3841 ggctatgtcg caacaggtgg cccgtcctga gtggctttgc gctcgctcat tgccaaatgc
     3901 aacacccggc caggcaacaa cagcgcgtaa tagaggccaa tgaacggaga tgtcaagaac
     3961 aaagccacgt tccgcagg
//
LOCUS       DAOSLL010000473         3966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_20090_length_3966_cov_4.407568, whole genome shotgun sequence.
ACCESSION   DAOSLL010000473 DAOSLL010000000
VERSION     DAOSLL010000473.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20090_length_3966_cov_4.407568"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            745..2040
                     /locus_tag="PLB25_17225"
     CDS             745..2040
                     /locus_tag="PLB25_17225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HOE43352.1"
                     /translation="MSVKSMYSHKKMTASDAISLVRDGDSIVVPTGVGEPPKLLTALS
                     EQRRKFRDVKVVQILAMRKFDYIDPETVDHVRHLALFYGGATRKGGQEGWIDFIPNYF
                     SKIPSMISRNQMPVDVVFSMASPMDSHGYFSLSLAADYTMAAVAKARAVVLEVNPHVP
                     FAFGNCHVHISQIAGLVESSEPVLEVGLPKIGAVQEAIGKYVADLIDDGSTLQIGYGG
                     IPDAVVMQLTAKHDLGIHTEMIGDGILTLLECGAVTNRRKNYLPGKMLATFALGSSKL
                     YKFMDRNPSLEIHPVNFTNMPALAGQNDNLIAINATLQIDLLGQCGSESLGHLPYSGT
                     GGQADFVRAANLSNGGKAFIVLPSTAKHHSISRIVPVMSPGTHFSISKNDVNYVVTEY
                     GVAQLRGKSGRQRAHELINIAHPDFRAELTEQAIKLRLV"
     gene            complement(2190..2753)
                     /locus_tag="PLB25_17230"
     CDS             complement(2190..2753)
                     /locus_tag="PLB25_17230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019572921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOE43353.1"
                     /translation="MTPRYTQAIHFAAAAHEGQRRKGTSIPYIVHPVAVAALVAEYGG
                     DEDQQIAALLHDVLEDGGPHFAPDIERRFGQRVLSIVQACTDGVPDANGVKQPWEIRK
                     RDYLEHLASASDDMLLVSACDKFSNAQAIVADLQKIGWAVFERFTAKRDGTIWYYQEL
                     SRVFSERNSPVASALKETVNKMVGFPN"
     gene            complement(2773..3021)
                     /locus_tag="PLB25_17235"
     CDS             complement(2773..3021)
                     /locus_tag="PLB25_17235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43354.1"
                     /translation="MSLLADKQRVWAGNGFVDYVLWGDDGKSLAVVEAKPTRRNAKVA
                     QQQAKLYVDCLQAQCGQRSVIFYSNGYETFMAAMKNSV"
     gene            complement(3523..>3966)
                     /locus_tag="PLB25_17240"
     CDS             complement(3523..>3966)
                     /locus_tag="PLB25_17240"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE43355.1"
                     /translation="VACARTSNNARREKTRFHGFVLVERTTRLLALQMNIEPPSSLDE
                     ILPVLTGCNLPVADISVSSPPQFFGFRVAGSVVAVVGLEHFQSVGLLRSLAVSPSHRG
                     HGLAQELVSFAESFAISSGVESLFLLTTTAEALFVKLGYCPASRQ"
BASE COUNT          866 a         1149 c         1123 g          828 t
ORIGIN      
        1 ggcgcgtgcg caagcggtcg agggcgaacg gaaccggcga tgaacgcgtc agtcgaagcg
       61 ctgcaatgag ctcgggaagc ggcagcttgc gcttgggcga tggcgcaggc tggtgtttgt
      121 cattggcatg tttgtgttgc ctgccagttg ctgctgccgt gctccggtgc agcttccagg
      181 ccgcaatact attgatagct gtcagcgcaa taacagtaag ggctggagca tgtttttgtg
      241 caaatttcag gggacggact gccttttcaa cgcggccttc caatcggaca gcttgccctg
      301 cgaaggcacc cggaaacacc agcctggcgc ctgtccgagg cgcgcccgac ctgcccgcac
      361 cgcaaaccca caccggtgct tcatgatgaa cgcctgggct gctcgccaaa cagggacacc
      421 tggagcctgt cgaacttgtg acatcaatcg ccagaaccta ctccagccgg ggccagtttg
      481 ggccgaattc gtcggcccat ggcccatcca tgggtcaaaa gggccagtgc aagacaagcc
      541 gctacggacg cttcgccgta gctgcgccat cagtccgcca ggccccaatc tggggcgaat
      601 atttttgatg aaaccccgcc tgtggtgctg gttcatcgta ataatgcctg tcactccgaa
      661 gcaatgagga acccggtcag tccagcattg gcggaccatc accgcttgtt gggctttttt
      721 tgttggatgt tcaaggggac aagtatgtcg gttaaaagta tgtattcgca taaaaaaatg
      781 actgccagtg atgcaatcag cctggtcagg gatggtgact cgattgtggt gcccactggt
      841 gtcggtgagc cacccaagct gctgacagcc ttgtccgagc agcgccgcaa gttccgcgat
      901 gtgaaggtgg ttcagattct ggcgatgcgc aagtttgact acatcgaccc cgaaaccgtg
      961 gaccatgtgc gccacctggc gctgttttat ggcggtgcca cgcgcaaggg tgggcaagag
     1021 ggctggatcg acttcatccc caactatttc tcaaagatcc ccagcatgat ctcgcgcaac
     1081 cagatgccgg tcgatgtggt tttcagcatg gcctccccga tggattcgca cggctatttt
     1141 tcgctcagcc tggcggccga ctacaccatg gcagcggtgg ccaaggcccg tgcggtggtg
     1201 ctggaagtca acccccacgt accgtttgcc tttggcaact gccacgtgca catctcgcag
     1261 attgctggcc tggtggaaag cagcgagcca gtgctcgaag ttggcctgcc aaaaattggc
     1321 gcagtacagg aagccattgg caagtatgtg gccgacctga ttgacgatgg ctcgaccctg
     1381 caaattggct atggtggcat acctgacgcg gtggtcatgc agcttaccgc caaacatgac
     1441 ctgggcattc ataccgagat gattggcgac ggcattttga cgctgctgga gtgtggtgcc
     1501 gtgaccaacc ggcgcaagaa ctaccttccc ggcaagatgc tggccacgtt cgcgctgggc
     1561 tcgtccaagc tttacaagtt catggaccgc aacccgtcgc tcgaaattca cccggtcaac
     1621 ttcaccaaca tgccggcgct ggccggacaa aatgacaatc tgatcgccat caacgccacg
     1681 ctgcagattg acctgttggg tcagtgtggc tccgagagcc tggggcatct gccctattca
     1741 ggtactggtg gccaggccga ctttgtccgg gcggccaacc tttcaaacgg cggcaaggcc
     1801 tttatcgtgc tgccgtccac cgccaaacac cacagcattt cacgcatcgt gccggtgatg
     1861 tcgccaggta cccacttcag catcagcaaa aacgatgtca actatgtggt gactgaatat
     1921 ggtgtggcgc agttgcgcgg caaatcaggc cgacagcgtg cccacgagct gatcaacatc
     1981 gcgcacccgg atttccgcgc cgagttgacc gagcaggcca tcaagctccg tttggtgtaa
     2041 caaaaaggcc tgtagccatt gatggcactg cgccagatgc tctgttaaac ggagcatttg
     2101 gcaaaatttg gttatccagc cttaaccctg gcacaggctc cacagccagc gccgccagca
     2161 actgttgaca tggttcttgt cgcgccttgc tagtttggga accctaccat cttgttcacg
     2221 gtctccttca gcgccgatgc gacgggtgag ttgcgttcgg aaaagacgcg cgacagttct
     2281 tggtagtacc agatcgtgcc atcgcgtttt gctgtaaacc gctcaaaaac tgcccagcca
     2341 atcttctgaa gatcagcaac aatggcttgg gcgttgctaa atttgtcgca ggcggaaacg
     2401 agcagcatgt cgtcggaagc gctcgccagg tgttctaggt aatcgcgctt gcgaatttcc
     2461 cacggctgct tcaccccatt ggcatcagga acaccatccg tgcaggcttg cacaattgac
     2521 agcacccttt gaccaaatcg ccgctcaatg tcaggcgcga aatgtgggcc gccatcttca
     2581 agaacgtcat gcagcagtgc cgcgatttgt tggtcctcgt cgccaccata ttctgcgacc
     2641 aacgcggcca cagcaacagg atgaacaatg tacggaatcg aagtcccttt tcggcgctgt
     2701 ccttcatgcg ccgctgcggc gaagtgaatg gcctgtgtat atcgcggtgt catattgatc
     2761 cccagagtca gttcagacgg agtttttcat tgcggccatg aaagtctcat agccattgga
     2821 atagaagatc accgaccgct gcccgcactg tgcttgcagg cagtcgacgt acagcttggc
     2881 ctgttgctgg gctaccttgg cattccggcg cgtgggtttg gcttccacca ccgccagcga
     2941 tttgccatcg tcaccccaca gcacgtaatc gacaaagcca ttgcctgccc atacccgctg
     3001 cttgtcagct aacagactca ccaatattaa tctgtgtgac tcctgcctcc acaagttaac
     3061 cgcaccgagt acggcgcaca cctggattcc ccggttgaac gcttgttgtc accggtaagc
     3121 ccctggcgta tttgttcttc aggtttgagg gggtccagct attgggtgac agcgctgcgc
     3181 gcccgcttga accgctggca acgcgtctgg gggcgttgcg cccgcctctt cccgccagca
     3241 cgacaagctg cttcagcaaa gtgcgtcgcc cctgcagggc gcagccatgc cccaaggctg
     3301 gcatacttga agccatccaa acattcgcgg gaagatttgg caccaaggct ttcgcccgac
     3361 ccaggcgcgt gcaacggagc gtagcggtca ccggtggagt gccgcaggcg cggagtccgc
     3421 gtgaaccgcc gggttagccc gcccgacacg cttggacaga aaggcagagg aggacggaca
     3481 aagcccagaa aactggggtg tagcctgaat ggcttgcgga gcctattggc gggaagcggg
     3541 acagtagccc agctttacaa aaagagcttc cgcagtagtg gtaagcagga acagtgactc
     3601 aacaccactc gagatggcaa aggactcggc aaaactcact agctcttgcg ctaaaccatg
     3661 cccgcgatga ctaggagaaa ctgcgagtga ccgaagtaag ccgaccgact gaaagtgttc
     3721 caagccgaca acagcgacca cggagccagc aacccggaaa ccgaagaact gtgggggtga
     3781 tgaaacagag atatcggcaa ccggcaaatt gcagccggtg agtacaggga gtatttcgtc
     3841 aagcgatgac ggtggttcga tgttcatttg aagagctaac aatctagtag tccgttccac
     3901 caaaacgaaa ccatgaaatc gtgtcttttc ccgcctggcg ttgttgctcg tcctggcaca
     3961 agctac
//
LOCUS       DAOSLL010000474         3966 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_20093_length_3966_cov_3.452570, whole genome shotgun sequence.
ACCESSION   DAOSLL010000474 DAOSLL010000000
VERSION     DAOSLL010000474.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3966)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3966
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20093_length_3966_cov_3.452570"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1635)
                     /locus_tag="PLB25_17245"
     CDS             complement(<1..1635)
                     /locus_tag="PLB25_17245"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOE43356.1"
                     /translation="MLLSSLNLLAQAPNGVARLRELILTLAVQGKLVPQDPKDEPASA
                     LLKKIRAEKDRLIAAKTLKREKDEPALTDDAPPFDLPVTWGWVALGEVIDIVRGITFP
                     ATQKTREAAPGRIACLRTTNVQQTIEWEDLLFVDRSFMGRADQLIQHHDIVMSMANSR
                     ELVGKVALIDQIPHAEATFGGFLGVLRPRKIDPLYAMAVLRTPYARSLLIDSSSQTTN
                     IANVSLAKLRPLPFPLPPEAEQSRIVTRVEELMRLCDALEAKGQLEATQHAQLVSTLL
                     ATLTDSETPAQLADNWHRIATHFDLLLDRPEAVDALEQAILQLAVRGLLVPQDPQDEP
                     ASELLKKIRAEKDKLIAEGKIKRDKPLPPIAEDEQLPMLPRGWEDVRLGDLIELVSGQ
                     HLGPNEYVEGLGGRVPYLTGPAEFGLISPEPSRSTNERRAIAIQADILITVKGSGVGK
                     LNVVTHPELAISRQLMAIRPVKLDSEFLMVVLQTMALHFQEKSVGIAIPGIGRDDVTH
                     TLIGLPPLAEQSRIVTRVAQLRHLCADLRQRLSASQS"
     gene            complement(1688..2770)
                     /locus_tag="PLB25_17250"
     CDS             complement(1688..2770)
                     /locus_tag="PLB25_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOE43357.1"
                     /translation="MKKPNTSLTASQATPVANVAEALYAQIQDVLQQARQQAKRSVNQ
                     AMVLAYWQVGKLIVEHEQGGEDRAVYGAQTLTTLAQRLTAEFGSGFAVQSLRNYRQFY
                     LTFPTDEIRSTPWSELGWSHIKTIMRVPDPAARQWYALEAVSQTWSVAALDRQISTLY
                     YQRLLSSQDQDGVKAEASALIQRDAPPSPRDFIRDPYILEFLDGQPQSSWYEKDVEQG
                     LLDQLQKFLLELGKGFAFVARQRHLRIEGEDAFVDLVFYNYILKCFVLIELKVGKLSH
                     QDVGQLDMYVRVFDEQVRQPGDNPTVGLILCSERNEAVARYSLLAGSEQIFASRYQPW
                     LPTEAELQAELTRDRALIENARGDQA"
     gene            complement(2829..>3966)
                     /locus_tag="PLB25_17255"
     CDS             complement(2829..>3966)
                     /locus_tag="PLB25_17255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HOE43358.1"
                     /translation="EDAYNYMKSGHLLRQVINKLNTIDFNRQAERHQFNDLYEKILKD
                     LQSAGNAGEFYTPRAVTQFMVDMVNPQLGEKVLDPATGTGGFLVCAIEHLRKQVQNTE
                     QEAVLQTSITGVEKKQLPHMLCVTNLMLHGIEVPSLITHGNTLARPLREVTQADRVDV
                     VLTNPPFGGVEEPGIEQGFPSDVRTKETADLFLVLIKHILKTHGRAALVLPDGTLFGE
                     GVKTRIKEQLLAECNLHTIVRLPNGVFAPYTGIKTNLLFFTKGTPTKEVWYYEHPYPA
                     GVKNYNKTKPIRIEEFDAEKAWWGSEADGFASRQENESAWKVSIEQIKAMGYNLDQKN
                     PHAADAVSHDPEQLLADYARLQGEAQALRDELKEILAKSLASPR"
BASE COUNT          704 a         1082 c         1266 g          914 t
ORIGIN      
        1 ggattggctg gcggacaggc gctggcgcag gtcggcgcac aggtggcgca gctgggctac
       61 gcgggtgacg atgcgggatt gttcggcgag gggtggcaaa ccaatcagcg tatgcgtcac
      121 atcatctcgc ccgattccag gaatcgcgat gccgactgac ttctcttgaa agtgcaacgc
      181 cattgtttgc agcacgacca tcagaaactc tgagtccaac ttcaccggcc gaatcgccat
      241 caactgccga ctgatagcca gttcaggatg cgtgacaacg ttgagttttc caactcctga
      301 gcccttaacc gtaataagta tgtcggcttg aatagcgatt gcacgccgct cattagttga
      361 gcgagatggt tctggtgaaa tcaagccgaa ttcagctggg cccgtcaagt acggaacgcg
      421 gccaccaagt ccttcgacat attcattggg tccgagatgt tgacccgaaa ccaattcaat
      481 caaatcaccc aaccgaacat cctcccagcc gcgcggcagc atagggagct gctcatcctc
      541 cgcaatcggc ggcaggggtt tgtcgcgttt gatcttgcct tcggcgatga gcttgtcttt
      601 ttctgcgcgg atttttttga gcagttcgct ggcgggttcg tcttgcgggt cttgggggac
      661 gagcaggccg cgcacggcga gttgcaggat ggcttgctcc agggcgtcca cggcttcggg
      721 gcggtccagc agcaggtcga agtgggtggc gatgcggtgc cagttgtcgg ccagttgcgc
      781 gggcgtttcg ctatcggtga gcgtggccag cagggtgctg acgagttggg cgtgttgcgt
      841 ggcttcgagc tggcctttgg cttcgagcgc gtcgcacagg cgcataagtt cttcgacgcg
      901 ggtgacgatg cgggattgtt cggcttctgg cggtagtgga aatggtaaag gccgcaactt
      961 agccaatgac acgttggcaa tgtttgtcgt ttggcttgaa ctgtcaatca atagcgaccg
     1021 cgcatatggc gtgcgcagaa ctgccattgc gtaaagtggg tcgatcttgc gcggacgcaa
     1081 gaccccgagg aagccaccaa aagttgcttc ggcgtgggga atttgatcga tgagcgcaac
     1141 cttgccaact aactcgcggc tgttcgccat ggacatgacg atgtcgtggt gttggataag
     1201 ttggtctgcg cgccccataa acgaacggtc gacgaacaag aggtcttccc actcgatggt
     1261 ctgttgaaca ttcgtagtgc gcagacaagc gattcgccct ggcgcagcct ccctggtttt
     1321 ctgcgttgcg gggaaagtaa taccgcgaac tatgtcgatg acctccccca acgcaaccca
     1381 accccaagtg acgggcaagt caaaaggcgg cgcgtcgtcc gttagcgcgg gctcatcctt
     1441 ttcgcgcttc agtgtcttcg cagcaatcag ccggtctttc tccgcccgta tcttcttcag
     1501 cagcgcactc gccggctcgt ccttggggtc ttggggcacc agcttgccct gcacggccag
     1561 cgtcaaaatc agctcgcgca gacgtgctac gccattgggg gcttgcgcca ataggttcaa
     1621 acttgatagc agcatgcgca aattccacgg gggctagagg ccaatttggt tgcaaaaatt
     1681 gggggcgtca cgcttgatcg cctcgggcgt tttctatcag cgcacggtcg cgggtcagct
     1741 cggcttgcag ctcggcctcg gtgggtagcc atggctggta acggctggca aagatttgtt
     1801 cgctgccagc cagcagcgag tagcgggcca ccgcctcgtt gcgctcggag cacaaaatta
     1861 aaccgaccgt ggggttgtcg ccaggctggc gcacctgttc gtcgaacacg cgcacgtaca
     1921 tgtcgagttg gcccacgtcc tggtggctga gcttgcccac tttcagctcg atgagcacaa
     1981 agcatttcag gatgtagttg taaaagacca gatcgacaaa ggcatcttcg ccttcaatgc
     2041 gcaggtggcg ctggcgagcc acgaaggcaa agcctttgcc cagctccagc aaaaattttt
     2101 gcaactggtc gagcaggcct tgctctacgt ctttttcgta ccagctggat tgcggctggc
     2161 cgtccagaaa ttccagaatg taggggtcgc ggatgaagtc acgcgggctg ggcggtgcgt
     2221 cacgctggat gagggcgctg gcttcggcct tgacgccatc ctggtcttgg ctggagagca
     2281 ggcgctggta atacagggtg ctgatttgcc ggtctagcgc ggccacgctc caggtctggc
     2341 tgacggcttc aagggcgtac cactggcggg cggcggggtc gggcacgcgc atgatggtct
     2401 tgatgtgcga ccagcccaat tcgctccacg gcgtggagcg aatctcgtcg gtggggaagg
     2461 tcagataaaa ctggcggtag ttgcgcaagc tctgtaccgc aaagccgctg ccgaactctg
     2521 ctgtcagacg ctgcgcgagg gttgtgagtg tttgcgctcc atacacagcg cggtcttcgc
     2581 cgccttgttc gtgctcgacg atcagtttgc caacctgcca gtaggccagc accatggcct
     2641 ggttgacgct gcgcttggct tgttggcggg cctgttggag cacatcttga atctgggcat
     2701 acagcgcctc tgccacgttg gccactggcg tggcctggct ggcggtcaag ctggtgttgg
     2761 gctttttcat ggcaatgctc tcatggcaaa tggtaggttc attaaatttg atagcagcat
     2821 gcgcacattc aacgggggct ggcgagtgat ttggccaaaa tttccttcag ctcatcgcgc
     2881 agggcctggg cctcaccttg caggcgggcg tagtcggcca gcagttgctc ggggtcgtgg
     2941 ctcaccgcat ctgcggcgtg ggggttcttc tggtccaggt tgtagcccat cgccttgatc
     3001 tgctcgatgc tgaccttcca ggcactttcg ttttcctgcc gagaagcaaa gccatccgcc
     3061 tcgctgcccc accaggcttt ctcggcatca aattcttcga tgcggatggg tttggtcttg
     3121 ttgtaattct tcacgcccgc tggatacggg tgctcgtagt accagacttc cttggtgggc
     3181 gtgcccttgg tgaagaacag caggttggtc ttgatgccgg tgtagggcgc gaacacgcca
     3241 ttgggcaggc gcacgatggt gtgcaggttg cactcggcca gtagctgctc tttgatacgg
     3301 gttttgacac cttcgccaaa cagggtgccg tcgggcagca ccagcgcggc gcggccatgg
     3361 gttttgagga tgtgtttgat gagcaccaaa aataaatccg ccgtttcttt ggtgcgcacg
     3421 tcgctgggaa agccttgctc aatgcccggc tcttccacgc cgccaaaggg cgggttggtg
     3481 agcaccacgt ccacgcggtc ggcctgcgtg acctcgcgca gcgggcgggc cagcgtgttg
     3541 ccgtgcgtga tgaggctggg cacctcgatg ccgtggagca tgaggttggt gacgcacagc
     3601 atgtggggca gctgcttttt ctccacaccg gtgatgctgg tttgcagcac ggcttcctgc
     3661 tcggtgtttt gtacctgttt gcgcaagtgc tcgatggcgc agactagaaa gccgccggtg
     3721 ccggtggcgg ggtcgagcac cttttcgcct aactgggggt tgaccatgtc caccatgaac
     3781 tgggtgacgg cgcgcggggt gtagaactcg cccgcgttgc cggcgctttg caggtctttg
     3841 aggatttttt cgtagaggtc gttgaactgg tggcgctcgg cctgtcggtt gaagtcgatg
     3901 gtgttgagct tgttgatgac ctggcgcagc aggtggccgc tcttcatgta gttgtaggcg
     3961 tcttcg
//
LOCUS       DAOSLL010000475         3950 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_20180_length_3950_cov_4.196662, whole genome shotgun sequence.
ACCESSION   DAOSLL010000475 DAOSLL010000000
VERSION     DAOSLL010000475.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3950)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20180_length_3950_cov_4.196662"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(260..1564)
                     /locus_tag="PLB25_17260"
     CDS             complement(260..1564)
                     /locus_tag="PLB25_17260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HOE43359.1"
                     /translation="MIIVLLFSLMFVLMALGLPIALSIGLPAIGLIVTPGVFPDTATL
                     AALGQTIVQQLFSGVDSFDLLAVPLFMIAGAIMEVGGISRRLIDFCDSLVGWAPGGLA
                     AAAIVASMIIAGISGSAAADTAAIGAVVIPAMIRQGYPPAFAAAVVAAGGAIGIIIPP
                     SIPMVIFGFMTNISTSQLFAGGMLVGLLMGLSFIAVAVFISWRRGYGQRQPFTWAKVA
                     LTGKAAIWSMGAPVVVLGGILTGTVTVTEAAALAVFYALLVGAVINRELAWRDLPGLI
                     IRSQVAAGGILFIIAMAKIFGWLLAMQQGAQLLDGFVNSLSLPPLGLLLLVMGMLLLV
                     GCVMETTAALLLLVPVLALLTPQMQVDPVQFGVLVVVNLAIGMLTPPVGICLFVSCGI
                     AGVSLGSISRAVAPLAAVAMLDLMIAALWSPLTMWLPTLIYR"
     gene            complement(1561..2043)
                     /locus_tag="PLB25_17265"
     CDS             complement(1561..2043)
                     /locus_tag="PLB25_17265"
                     /inference="COORDINATES: protein motif:HMM:NF016200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HOE43360.1"
                     /translation="MSLNHRLLVISERVNSLVEKLLLAAGVTISVVLFAQVLARYLGH
                     SLSWSEEIGRYLLVATTFLGATVAYKRAHFIGLAGFGARFGLLVEQIIVRSLQVLTLA
                     CFALITWYGVIYTVKSWDQTSTAIQMPMSLPISVVPLSGAIFLLHILADMTRRKEDAK
                     "
     gene            complement(2109..3089)
                     /locus_tag="PLB25_17270"
     CDS             complement(2109..3089)
                     /locus_tag="PLB25_17270"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005488 - binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DctP family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOE43361.1"
                     /translation="MSFISRTLTGLGFAASLVFAAPANAAEKVNIGVVTGPTFVHTIA
                     AQKFREEVDKALPGKYEVVVHHSAALGSETQVLQQLQLGTVQMSVCTTGPIEAFVPEI
                     KALELPFLFTSYEASDKVLDGPIGQGLLKRFDKAGLVGMHFLDNGFRNVTNSKRAVNG
                     PDDLKGMKIRTMESPTHLAIWRAIGANPTPMAWPITTQLQQGVLDGQENPISVISAGK
                     LNEAGQKYLSLTRHVYSALVFVGSKAFIDKLPAADRKVFMDAARSAALTGRRYVRDNE
                     ASQLAGLKAAGMLVVETPDVAAFRTKTESVYASLGGDTKKIVEDIRQTLK"
     gene            complement(3315..>3950)
                     /locus_tag="PLB25_17275"
     CDS             complement(3315..>3950)
                     /locus_tag="PLB25_17275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HOE43362.1"
                     /translation="PNEFTPSLIAAYDKLPKLVSHLHLPVQHGSDRILMAMKRGYTAM
                     EYKSTVRKLRAIRPDMAMSSDFIVGFPGETEEDFGKMMKLIDEVGFDNSFSFIFSPRP
                     GTPAANLPDDTPHEVKLRRLQHLQGVINASIKHISESRLGTVQRILVEGAARRDTTEL
                     MGRTECNRVVNFAGQPRLIGQMVDVLITETRTFTLRGEVLTNGARTSTMAT"
BASE COUNT          869 a         1170 c         1186 g          725 t
ORIGIN      
        1 gagcaccaag gccgagccag cgctgatgag aatttaccaa gcgctgggga tcagtcaagc
       61 accaggcggc accaaaaaac ttgtggtttg acgcgagaaa tacgagcaaa tctgacaact
      121 gaaaactagc acgatctaag ttgttgattt ttaaaggtat ttcttgatgg ttgttaaagt
      181 ccggttaagg ctccagccct tgtcaaatat gcggtagcag ctatctttat ttaagcattc
      241 cgatgccgct ttgacagcct cagcggtaaa tcagggtcgg tagccacatg gtcagcggcg
      301 accacagggc ggcgatcatc aggtccagca tggcaactgc cgccaggggt gccaccgcgc
      361 gtgaaatgct gcccagcgac accccggcaa tgccacaact gacaaacagg caaatgccga
      421 ctggcggcgt cagcatgcca atcgccaggt tgaccaccac cagcaccccg aactgcaccg
      481 gatcaacctg catctgcggt gtcagcaagg ccagcaccgg caccagcaac agcagggcgg
      541 cggtggtttc catcacgcag cccaccagca gcagcatgcc catcaccagc agcagcaggc
      601 cgagcggtgg cagcgagagt gaattgacaa acccgtcgag cagttgggca ccctgctgca
      661 tcgccagcag ccagccaaag attttggcca tggcaatgat gaacaaaatg ccaccggctg
      721 ccacctgcga gcgaatgatg aggccgggca agtctctcca ggccagttca cggttgatga
      781 cggcacccac cagcagcgca taaaacaccg ccagcgcggc ggcttcggtc accgtgaccg
      841 tgcccgtcaa aatgccacca agcaccacaa ccggcgcacc catcgaccag atcgcggcct
      901 tgccagtcag cgctaccttg gcccaggtaa acggctggcg ctggccgtaa cccctgcgcc
      961 acgagatgaa aaccgccacc gcaatgaatg acagccccat cagcaggccg accagcatgc
     1021 caccggcaaa cagttgtgaa gtcgaaatat tcgtcatgaa accaaagatc accatcggaa
     1081 tcgacggtgg aatgatgatg ccgatggcgc caccggcggc caccacggcc gctgcaaacg
     1141 caggcgggta gccctggcgg atcatggccg gaatcaccac cgcgccaatc gctgcggtgt
     1201 ctgccgcagc cgagccggaa atgccggcga tgatcatcga ggcaacgatg gcggcagccg
     1261 ccagcccgcc gggtgcccag ccgaccaggc tgtcgcaaaa atcaatcagc cgccttgaga
     1321 tgccgccaac ttccatgatg gccccggcaa tcatgaacag cggcacggcc agcagatcga
     1381 acgaatcaac cccggaaaac agttgctgca cgatggtctg gcccaacgcc gccagcgttg
     1441 cggtgtcggg aaaaacccct ggtgtgacga tcagcccgat cgctggcagg ccaatcgaca
     1501 gggcaatcgg caggcccagc gccatcagca caaacatcag ggaaaacagc aggacgatga
     1561 tcattttgcg tcctccttgc gccgggtcat gtcggccaga atgtgcagca ggaaaatggc
     1621 accagacagc ggcacgaccg aaatcggcag cgacatcggc atctggatgg cagttgaggt
     1681 ctggtcccag gatttgacgg tgtaaatcac cccgtaccag gtgatcagcg cgaaacaggc
     1741 cagcgtcagc acctgcaggc tgcgcacgat gatctgctct accagcaggc caaatcgcgc
     1801 gccaaaaccg gccaggccaa tgaaatgggc gcgtttgtag gccaccgtgg cacccagaaa
     1861 ggtcgtggcc accagcaggt agcggccaat ttcctctgac cagctcagcg aatggccaag
     1921 gtaacgcgcc agcacctggg caaacaggac gaccgaaatc gtgacaccgg ctgccagcag
     1981 cagcttttca accaggctgt taacccgctc gctgatgacc agcagccgat ggttcaggct
     2041 catgaactct ccgagaggac aaaattggca tggcggcaag cgccatgctg cggcgcaaca
     2101 gtggccgctt acttgagggt ttggcggatg tcttcgacaa tttttttggt gtcgccgcca
     2161 agcgacgcgt aaaccgactc ggtcttggtc cggaaagccg ccacgtcagg ggtttcgacc
     2221 accagcatgc cagcggcctt caggccggcc aactggctgg cttcgttgtc acgcacatag
     2281 cgccggccag tcagggcggc cgacctggca gcatccatga acaccttgcg gtcggcggcg
     2341 ggcagtttgt cgatgaaagc cttgctgccg acaaacacca gcgcggaata gacgtgccgg
     2401 gtcagcgaca ggtatttttg accagcttcg ttgagcttgc ctgccgaaat caccgaaatc
     2461 gggttttcct gaccatcgag cacaccttgt tgcagttgcg tggtgattgg ccaggccatc
     2521 ggggtcgggt tggcaccaat ggcgcgccag attgccaggt gggtgggcga ctccatggtg
     2581 cgaatcttca tgcccttgag gtcgtccggg ccgttgacgg cgcgcttgga gttggtgaca
     2641 ttgcgaaagc cgttgtccag aaagtgcatg ccaaccagcc cggccttgtc aaagcgcttg
     2701 agcaaaccct ggccaatcgg gccgtcgagc actttgtcgg aggcttcgta agaggtgaac
     2761 aggaaaggca gttcaagcgc cttgatttca ggcacaaaag cctcgatcgg gccagtggtg
     2821 caaacgctca tctgcaccgt gcccaactga agctgctgca gcacctgggt ttcgctgccc
     2881 agcgcagccg agtggtgcac caccacctca tacttgccgg gcagggcctt gtcaacttct
     2941 tcccggaact tctgggccgc aatggtgtgg acaaaggttg ggccggtaac cacgccgatg
     3001 ttgacctttt cggcggcatt ggccggtgcg gcaaacacca ggctggcggc aaagcccagt
     3061 ccggtcaggg tgcgtgaaat gaatgacatg gtgtttttcc ttgaatggca aaagtggtgt
     3121 tttatgtgtc tgctgcgaag ggttgcaagg cgctgaaagc ggtcgatcat acaggcagac
     3181 cggggcaggc aggtggctcg gacgagcaga ctattgccca agcactggtc ccggcatttc
     3241 aagtgacttt ttgggctgca gcccttgccc ttgttgcgta tctagctcct gaaatagtag
     3301 taatttgagg tccttcaggt ggccatggtg gatgttcgtg caccgttggt cagcacttcg
     3361 ccgcgcagcg tgaaggtgcg ggtttcggtg atgagcacgt ccaccatctg cccgatcagg
     3421 cgtggctggc cggcaaagtt gacgacccgg ttgcattcgg tgcggcccat cagttcggtg
     3481 gtgtcgcgcc gcgccgcacc ttcgaccaga atgcgctgca cggtgccgag gcggctttcg
     3541 gaaatgtgtt tgatgctggc gttgatcacg ccttgcaggt gctgcaggcg gcgcagcttg
     3601 acctcgtgcg gggtgtcgtc gggcagattg gctgcaggtg tgccagggcg cgggctgaag
     3661 atgaagctaa agctgttgtc aaagccgact tcgtcaatca gcttcatcat cttgccaaag
     3721 tcttcttcgg tctcacccgg aaaacccaca atgaagtcgc tgctcatggc catgtcgggg
     3781 cgaatcgcgc gcagcttgcg caccgtgctc ttgtattcca tagcggtgta gccgcgcttc
     3841 atggccatca aaatccggtc cgagccgtgt tgcaccggca agtgcaggtg gctgaccagt
     3901 ttgggcagtt tgtcgtaggc cgcaatcagc gacggcgtga actcgttcgg
//
LOCUS       DAOSLL010000476         3937 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_20278_length_3937_cov_4.315817, whole genome shotgun sequence.
ACCESSION   DAOSLL010000476 DAOSLL010000000
VERSION     DAOSLL010000476.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20278_length_3937_cov_4.315817"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..2304)
                     /locus_tag="PLB25_17280"
     CDS             complement(64..2304)
                     /locus_tag="PLB25_17280"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE43363.1"
                     /translation="MNKESFFGLGLAFVCNFDGKVIEVAWDDFGIEKKLLEPAHFVCV
                     FDAASVKKGLNFFLEVKEHGAAFDWEIDVSVDERPRAFNFSAVSLGDRIVVLASISGE
                     GDNKIYDGLSQVINKQVTSLRKLSKQQSSQIDAKADKNAIPDQVKVEMITDMMTLNNR
                     LVNAERELARKHAELKRMSASVSRDLHLAQRVLHFSGEAVMIGDRNRKVIDVNSAFTA
                     MTGFSKAEVVNSALVLSEPACDMTKTNEGILQAVADRGLWQGECMGMRKNGTLFPKWL
                     SVSVIPDDTGEVGHYVVNFSDISRLKFAEEQWQRLAYFDSLTNLPNRALFKDRLQQAV
                     VKAARMGESLLLLFIDLDEFKVVNDSLGHDVGDLLLGEAARRLEACIRKADSIYRLGG
                     DEFTVIVNGCHDDLNALHLCEKIIETLKLPFLIQGNWIHIGASIGIARHPLDGESADE
                     LTKSADTAMYAAKASGRNRSCYFSKSLGEKVATQLNLRAQIEQGLRRGEFLVHLQPEM
                     NLLTGEVIAMEALARWQHPSRGLLGPEDFISVAEEFGLILELGEFVLCEALRMVRGLR
                     DLGWIKLRVSVNVSSRQLASPDFLNTVLNRLREQALPGAALIVEITESMVLGNLEHAI
                     QVLKTLKEQGIHAAIDDFGTGYSSLSHLHRLPVEYLKIDQSFVADIDVDQDSQTIVRT
                     ILAMAKSLGIMTVAEGVERPAHLAILSELGCNIGQGFLYSRPMPYEKFQGFIEQNQQE
                     RAYSGL"
     gene            complement(2310..3344)
                     /locus_tag="PLB25_17285"
     CDS             complement(2310..3344)
                     /locus_tag="PLB25_17285"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HOE43364.1"
                     /translation="MVISSETLLLENRSEFAALVRARLEASLLAFGANERLRMVFDLE
                     MHLAFLGEAVRCDVASIFVEYAIWTNQLLLTCGGDSLDFRACLLAMDAQLQESGCGDW
                     VNVARRDLAEGLRQLDTAQPVAKSYLTPDNPHLAVANAFLNDCLNLRRGGALDGIQNA
                     IKTGISVRDIYLHVITPVMHELGCLWHRNKITIGLEHYCTAVAQMVMAQLFPLLFDGA
                     AKKKRLVSACVTGELHEIGVRMVSDLFELNGWDTVFLGADVPVDSVVDVIVQHNASVL
                     AISVTMGANLSALSRLIAAVRDSPACANVKILVGGAAFNVDGSLWQRLGADGWAADVQ
                     IAIKLANAWK"
     gene            complement(3525..>3937)
                     /locus_tag="PLB25_17290"
     CDS             complement(3525..>3937)
                     /locus_tag="PLB25_17290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HOE43365.1"
                     /translation="GLPGALLPFEQGSFLQHLEVPGVWLERGRLDATDSRLMNGQLGL
                     AGHRCLASLFFVAGAALERNRREAALALARDIVAAHILNPSAGVTCPNSQVMVLRVLS
                     PLVEPAVNLLRQVWLAWRAHFWQLLAVNPRLWST"
BASE COUNT          928 a         1179 c          990 g          840 t
ORIGIN      
        1 atgcctgacc aagactgtca gcaaagatcg acaacatgtt ggtaagtttg atgcgcatag
       61 cgcctaaaga ccggaataag cacgctcttg ttgattctgc tctatgaaac cctgaaattt
      121 ctcatacggc atcggtctcg aatacaaaaa gccttgtccg atgttgcaac caagttcgct
      181 gagaatggcc aggtgtgcgg ggcgttcgac accttcggcg acggtcatga tcccgagact
      241 ttttgccatg gcgagaatgg tgcggacaat cgtctgactg tcctgatcca catcgatatc
      301 tgcaacaaat gattggtcaa ttttgaggta ttccactggc agccggtgca aatggctcaa
      361 tgacgaatag ccggtgccaa aatcatcaat cgctgcgtgg atgccctgct ccttgagtgt
      421 tttcagcacc tgaatggcat gttccaggtt gcctaacacc atgctttctg tgatctcgac
      481 gatcagtgcc gcgccaggca aagcctgttc ccgcagacga ttcaacaccg tgttcaggaa
      541 atcaggagaa gcgagctgtc ggcttgacac attcacactc acgcgaagct tgatccagcc
      601 cagatcacgc aacccgcgaa ccatccgcaa tgcctcgcac agcacaaact cgcctagttc
      661 gaggatcaag ccgaactctt ctgcgactga aatgaagtcc tccggaccga gcagtccacg
      721 gctgggatgc tgccaacgcg ccagcgcctc catggcgatg acttcgccag tcaaaagatt
      781 catttccggc tgcaggtgga ccaaaaactc gccacgccgc aggccctgtt caatctgagc
      841 ccgcagattg agttgcgtcg caactttctc accgagggat ttcgagaaat aacaactgcg
      901 gttgcggcca ctggccttgg ctgcgtacat ggctgtatcg gcacttttgg tcaattcgtc
      961 ggcgctttcg ccatccagcg ggtggcgtgc gatgccgatg ctggcaccaa tgtggatcca
     1021 attgccctga atcaaaaatg gcagcttcag ggtttcgatg atcttttcgc acaaatgcaa
     1081 ggcgttcagg tcgtcatgac agccgttcac aatcacggta aattcgtcac cgcccaatcg
     1141 atagatgcta tcggctttgc gtatgcaggc ctcaagcctg cgagctgctt cacccagcaa
     1201 cagatcacca acatcgtgcc ccaggctgtc attgacgacc ttgaactcgt ccagatcgat
     1261 gaacaacagc aacaatgact cacccatgcg agcggctttg acaacggctt gttgcaggcg
     1321 gtccttgaac agggcccggt taggtaaatt ggtcagactg tcgaaatagg ccagacgctg
     1381 ccattgctct tccgcaaact tcaggcggct gatgtcggag aaattgacca catagtggcc
     1441 gacttcgccg gtgtcgtccg ggatcacgct caccgacagc catttcggaa acaaagtgcc
     1501 gttttttcgc atgcccatgc actcaccttg ccaaaggccc cggtccgcta ccgcctgaag
     1561 aatgccttcg ttggttttgg tcatgtcgca tgctggctca ctcagcacca gggcgctatt
     1621 gaccacctcg gccttgctaa agccagtcat ggcggtaaac gccgaattga catcaatgac
     1681 cttgcggttt cgatcaccaa tcatgaccgc ctcacctgaa aaatgcagca cgcgctgggc
     1741 caggtgcagg tccctgctga cgcttgcgct catgcgcttg agctcggcgt gtttacgcgc
     1801 cagttcccgc tcggcgttca ccaaccggtt gttcaaggtc atcatgtcgg taatcatttc
     1861 caccttgacc tgatccggga tcgcgttctt gtcggctttt gcgtctatct gggaactctg
     1921 ctgcttgctc agtttgcgca gggatgtgac ctgtttgttg atgacctggc ttaacccgtc
     1981 gtagatcttg ttgtctccct cgcccgatat cgatgcaagc accacaatcc ggtcccccag
     2041 ggaaacagcg gaaaaattga acgcgcgggg ccgttcatcc acgctgacat cgatttccca
     2101 atcgaacgca gcgccatgct ccttgacctc gagaaagaaa ttcaacccct ttttgaccga
     2161 cgcagcatcg aatacacaga cgaaatgcgc tggttccagg agctttttct caataccaaa
     2221 atcatcccag gcgacctcga tcaccttgcc atcaaaatta cagacaaaag ccaggcccaa
     2281 gccaaaaaaa gattctttat tcatgcctac tacttccacg cgttagcgag cttgatggca
     2341 atttgtacat ctgctgccca accatctgcg cccagccgtt gccacagcga accgtcaaca
     2401 ttaaaggccg ccccgccgac caaaatcttc acattggcac aggctgggct gtcacgaaca
     2461 gccgcgatca accgggacaa cgcactcaaa ttggcaccca tcgtgaccga aatcgccaaa
     2521 acactggcgt tgtgctggac gatgacatcg actaccgaat ccaccggcac atcggcaccc
     2581 agaaatacgg tgtcccagcc gttgagttcg aacagatcgg acaccatacg cacgccgatt
     2641 tcatgcagct caccggtaac gcaggcagaa acgagacgct tctttttagc cgccccatcg
     2701 aacagcagtg gaaacagttg ggccatcacc atctgcgcca ccgcggtgca gtagtgctca
     2761 aggccgatgg ttattttgtt gcgatgccac agacacccca gttcgtgcat caccggggtg
     2821 atgacgtgaa ggtagatatc cctgaccgat atgccagtct tgatggcgtt ttgaatgcca
     2881 tcgagcgcgc cgccacgccg caggttcaag caatcgttca gaaaagcgtt ggctacagcc
     2941 aggtgcgggt tgtccggggt aaggtaggac tttgcaacgg gttgggcagt gtcgagttgg
     3001 cgcaacccct cggccaggtc ccggcgggcc acgttcaccc agtcaccgca acctgattcc
     3061 tgaagttgtg cgtccatcgc caacagacaa gctctgaaat caagtgaatc gccgccacaa
     3121 gtcagcaaca gttggttggt ccagatcgcg tattcaacaa agatactggc cacatcgcaa
     3181 cgaaccgctt cgcccagaaa tgccagatgc atctccagat caaagaccat tcgcaacctt
     3241 tcgttggcgc cgaaggcaag caggctggct tccagtctcg cccgaacgag cgctgcaaac
     3301 tcgcttcgat tctcaagcaa cagtgtttct gaactgatca ccatgtgcgt ctccaaatta
     3361 ctgcccaaca ttgctatcga catcagtagc tgagtcgcca tagttcctgc acgactggat
     3421 taaacatcaa caggatgaat cgcgtcgccc cttttatttc gaacaagtcg acttggaaca
     3481 gaaattcatc gcaaactgac cccggcaatc tgcacctcag gcactcatgt tgaccataat
     3541 cttgggttta ccgcaagcag ttgccaaaaa tgcgcacgcc aggccagcca gacctgtcgc
     3601 aacaaattga cggcgggctc caccagcggt gacagcaccc gcagcaccat cacctggctg
     3661 ttcgggcagg tgaccccggc gctggggttg aggatgtggg cggcaacaat gtcgcgggcc
     3721 agcgccagtg cggcttcgcg ccggtttcgt tccagtgcag ccccggcgac aaaaaacagc
     3781 gaggccaggc agcgatggcc agccagccca agctggccgt tcatcagcct ggagtcggtc
     3841 gcatccaggc gaccgcgctc taaccataca ccgggtactt caaggtgctg caagaaactg
     3901 ccctgctcga acggcagcaa cgcgcccggt agtccga
//
LOCUS       DAOSLL010000477         3929 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_20321_length_3929_cov_5.061177, whole genome shotgun sequence.
ACCESSION   DAOSLL010000477 DAOSLL010000000
VERSION     DAOSLL010000477.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20321_length_3929_cov_5.061177"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1975)
                     /locus_tag="PLB25_17295"
     CDS             complement(<1..1975)
                     /locus_tag="PLB25_17295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOE43366.1"
                     /translation="MTIHAALNHVTHYSYDRLVNLGPQTIRLRPAPHCRSKIISYSLK
                     VEPEGHFINWQQDPFANYQARLVFPDKATEFKVTVDVVMDMAVYNPFDFFLEPDAEEF
                     PFNYEANLKQELAPYLTADPVTPLLQTQLDKIDRTKRRSVIFLVDLNTMVHHDIKYTI
                     RMEPGVQTPEETLSLKSGSCRDSAWLMVQLLRNCGLAARFVSGYLIQLKPDVKSLDGP
                     SGTEVDFTDLHAWCEVYLPGAGWVGLDATSGLLAGEGHIPLACTPQPSGAAPIEGGVD
                     ESEVEFAHHMAVTRIYEAPRVTKPYTEEQWAAVMALGEAVDQDLIDSDVRMTMGGEPT
                     FVAVNDRDAPEWNTDALGPTKRGFATELVHKLRQEYGQGGFLHFGQGKWYPGEQLPRW
                     ALNIYWRKDREPVWTNPALFADERVPSHYTSKDAQRFTTLLAAKLGVTPKFIQTAYED
                     TWYYLWRERRLPVNVDPFNSRLDDEMERARLRRVFEQKLDSPIGYVLPIKVAGYAECY
                     GAAWTTGSWFLRDERMYLMPGDSPMGLRLPLDSLPWVSEADFPYMIEQDPSTLRDPLP
                     AYGTVAARYAPGRAGLALGAAADQVRVVMPLNPQAYAPGGLAEQLIVWGTTQISRYKL
                     YPIYTTLSTDGEKTLTMAEVLSAVGTVSVTRALT"
     gene            complement(2466..3917)
                     /locus_tag="PLB25_17300"
     CDS             complement(2466..3917)
                     /locus_tag="PLB25_17300"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HOE43367.1"
                     /translation="MKRRHFVQGGSLVLLLGLQQLARGASILAVRVWPAADYSRVTIE
                     SDTKISFTQNFVPNPPRLAVDVRGIELNPTLKELVAKVGPSDPTINGIRVGQFGPGVV
                     RLVVDLKQPVAPQVFTLAPVAAYRHRLVFDLYPTAIQDPLETLIAERLKDAAPSPLGT
                     PPVAAGSAASAAPASDPLGELMAQKGGKTSPASHPAPALAATSSTQNAALSIATSASS
                     ERATAQKGMRGSPGTSMRETPPAATDRLIIIALDPGHGGEDPGAIGPNGTREKDVVLR
                     LAHRLRKRINQGSVNGNAMRAYLTRDADFFVPLHTRVDKARRVQADLFISLHADAFFT
                     PNPQGASVFALSQGGASSAAARWMADKENKADLIGGLNVKAKDAQVTHALLDMSTTAQ
                     IKDSLQLGGAMLGEIKRVGKLHKPKVEQASFAVLKAPDIPSVLVEAAFISNPEEEAKL
                     NSDAYLEQLADALMRGIDRYFSKNPPLARSRSI"
BASE COUNT          751 a         1183 c         1208 g          787 t
ORIGIN      
        1 ccgtcagcgc tcgggtcacg ctcaccgtgc ccaccgctga caacacctcg gccatggtca
       61 gggtcttttc cccatccgtg ctgagcgtgg tgtaaatggg atagagcttg tagcggctga
      121 tctgcgtcgt cccccagacg atgagctgct ccgcaaggcc gcccggcgcg taagcctgcg
      181 gattcaaggg catcacaacc cgcacctgat ccgctgccgc accgagcgcc agtccggccc
      241 tgcccggcgc atagcgggct gcgaccgtgc cgtaagcggg caacgggtcg cgcagggtag
      301 aggggtcttg ctcgatcatg taaggaaaat ctgcctcgct gacccagggc agcgaatcaa
      361 gtggcaggcg cagccccatc ggcgaatcgc cgggcatcag gtacatgcgt tcatcgcgca
      421 aaaaccacga accggtggtc caggccgcgc cataacactc ggcataaccg gccaccttga
      481 ttggcagcac gtacccgatt ggcgagtcca gcttttgctc aaacacccgg cgcagacgag
      541 cacgttccat ctcgtcatcc agcctggaat tgaacggatc gacattgacc ggcaggcgac
      601 gctcgcgcca caggtaatac caggtgtctt cataggcggt ctggatgaac ttgggtgtca
      661 cgccaagctt ggcggccagc agcgtggtga agcgctgcgc atccttactg gtgtagtggc
      721 tgggcacacg ttcatcggca aacagcgccg ggttggtcca gaccggttca cggtccttgc
      781 gccagtaaat gttcaaggcc cagcgtggca gttgctcacc cggataccac ttgccctgcc
      841 cgaagtgcag aaaaccgccc tgaccgtatt cctggcgcag cttgtgcacc aattcggtgg
      901 caaaaccgcg ctttgtcggc cccagtgcat cggtgttcca ttcaggtgca tcacggtcat
      961 tgaccgccac aaaagtgggc tcgccgccca tcgtcatgcg cacgtcgctg tcaatcaggt
     1021 cttgatcaac cgcttcgcca agcgccatga cagcagccca ttgctcttcg gtatagggtt
     1081 tggtaactcg cggggcctca taaatccggg tgaccgccat gtgatgggca aactccacct
     1141 cggactcatc gacaccgccc tcaatcggtg cagcaccgga tggctgcggc gtgcaagcca
     1201 gcggaatatg gccttcgccc gccagcagac ccgaagtggc atcgagccca acccagccgg
     1261 caccgggcaa atagacctcg caccaggcgt gcaggtcggt aaagtccacc tcggtgccgc
     1321 ttgggccgtc gagtgacttc acatcgggct tgagctgaat cagatagccc gacacaaagc
     1381 gcgccgccag cccgcagttg cgcagcagtt gcaccatcag ccaggccgag tcgcggcacg
     1441 agcctgattt caaagacagc gtttcttcgg gtgtttgcac ccctggctcc atgcggatgg
     1501 tgtacttgat gtcatggtgg accatggtgt tgaggtccac caggaagatc acgctacggc
     1561 gcttggtgcg gtcaatcttg tcaagctgcg tctgcagcag cggcgtcacc gggtcggcgg
     1621 tcaggtaggg cgcgagttcc tgcttgaggt tggcctcata gttgaacgga aactcttcgg
     1681 catcgggctc cagaaagaag tcaaacgggt tgtagaccgc catgtccatc accacatcga
     1741 ccgtgacctt gaattctgtg gccttgtccg gaaagaccag ccgagcctgg taattggcaa
     1801 acgggtcctg ctgccagttg ataaagtggc cttcaggctc gaccttgagc gaataggaaa
     1861 tgatcttgct gcggcagtgc ggggcagggc gcaggcgaat ggtttgcggg cccaagttaa
     1921 ccagccggtc gtaggagtaa tgggtaacgt ggttcagcgc agcgtgaatg gtcatggaga
     1981 ggtctcaaaa aggtcccaaa agagcgggcc ttgattcaaa taaacgggac gtttgacaac
     2041 tgtcagatac aagcaatgtt agtgccagag ccctgccagg agcctgtcgg actcgtggcg
     2101 catcggcggc gaaccgacca cagcagccca ttttgcactg gctttttgac ccatcgccgg
     2161 gacggggcct gcaaacccgg cagaaactgg cgtggccggg gtcgattctc gctgttgctg
     2221 ccaatagtcc gacggacccc atcgggatga cggcgcgaaa atgccgcctc gaggcatcat
     2281 gattgctatg ttttttattg ctgctgacgc atgtacaacg ggggctgcaa gccgatttat
     2341 tcattatttg cagacaagct ttaatcaata cgctgccggt ttgccacgcc cggtgtttga
     2401 gttttggcgc tgaagaccgg ttgctgccgg gcaaccagga atgccatggg ttggcagcgg
     2461 cgtattcaga tactgcggct acgtgccagt ggcggatttt tcgagaaata gcgatcaatg
     2521 ccgcgcatca atgcgtcggc cagttgctcc aggtacgcgt cgctgttgag cttggcctct
     2581 tcttccgggt tgctgataaa ggccgcttcg accagcacgc tgggaatgtc cggggccttg
     2641 agcacggcaa aagaggcctg ttcgaccttt ggcttgtgca gtttgccgac gcgtttgatc
     2701 tcgccgagca tggcaccgcc gagctgcaag ctgtccttga tctgcgcggt ggtgctcatg
     2761 tcgagcaggg cgtgcgtcac ctgcgcgtcc ttggccttga cgttcagacc cccaatcagg
     2821 tcggccttgt tttccttgtc ggccatccag cgcgccgcgg cgctggaggc gccgccctgg
     2881 ctcagcgcaa acacactggc cccttgcggg ttgggggtga aaaaagcgtc ggcgtgcagg
     2941 ctgatgaaca aatcagcctg aacccgccgc gccttgtcca cccgggtatg cagcggcaca
     3001 aaaaaatcgg catcacgggt caggtaggct cgcatggcgt tgccgttgac cgagccttgg
     3061 ttgatgcgct tgcgcagtcg gtgtgccagc cgcagcacca cgtctttttc acgcgtgccg
     3121 ttggggccaa tggcacccgg gtcttcgcca ccatggccag ggtcgagtgc aatgatgatc
     3181 agccggtcag tcgcagcagg cggcgtttcc cgcatgctgg tgccgggtga acctctcatg
     3241 cctttttggg ctgtagccct ttcagagctt gcgctggtag ctattgataa tgcagcattt
     3301 tgagtgctgc tggtggccgc caaggcgggg gcagggtgac tggccgggct ggttttaccg
     3361 cctttttgcg ccatcagttc acccagcggg tcgctcgccg gggccgcgct ggctgcagag
     3421 ccagcggcaa ccgggggtgt gcccaacggt gacggtgcgg catccttgag ccgctccgca
     3481 atcagcgtct ccagcgggtc ttggattgcg gtcggataca aatcaaacac cagccgatgt
     3541 cgatacgccg ccaccggcgc cagggtaaag acctgcggcg ctaccggctg cttcaggtcc
     3601 accaccagcc gcaccacacc tgggccaaat tgcccgaccc ggatgccgtt gatggtgggg
     3661 tcggacggtc caaccttggc caccagttct ttcagggtcg ggttgagttc aatgccacgc
     3721 acatcaacgg ccaggcgcgg cgggtttggc acaaagttct gggtgaagct gatctttgtg
     3781 tccgactcga tggtgacgcg cgaataatct gccgctggcc atacccgcac cgccagtatg
     3841 ctggcaccgc gcgccagttg ctgcaggccc agcagcagca ccaaagagcc accttgaaca
     3901 aagtggcggc gtttcatggg cagcctccc
//
LOCUS       DAOSLL010000478         3924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_20354_length_3924_cov_4.551564, whole genome shotgun sequence.
ACCESSION   DAOSLL010000478 DAOSLL010000000
VERSION     DAOSLL010000478.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3924
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20354_length_3924_cov_4.551564"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..857)
                     /locus_tag="PLB25_17305"
     CDS             complement(12..857)
                     /locus_tag="PLB25_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HOE43368.1"
                     /translation="MSRNLAASVRARLKQHADATKQDFNLTLTHYGLERLLYRLSVSE
                     HASNFLLKGALLFTFWYDVAHRPTRDADLLGFGPDDADTAVRTFREICLTPAEDGVVF
                     DPDSVKGSIIRKEAGYGGVRIDIQAKLDGARIALQVDIGFGDAVTPAPQAISYPVLLE
                     DFPAPQLRAYPKYTVVAEKFHAVCLLGLANTRMKDYFDLWVLLDDTTLDPSEVRRAIE
                     ATFERRKMPMPTTVPVGFTDTFANDATKQTQWKAFLKKNQLDPIVLADVVNKTGNPPA
                     LPGRQ"
     gene            complement(854..1471)
                     /locus_tag="PLB25_17310"
     CDS             complement(854..1471)
                     /locus_tag="PLB25_17310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003274020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HOE43369.1"
                     /translation="MNPRSHSTQEQAVLELARVRLLLRARDLAQQELPTIVLSRLVSA
                     GKLERVARGVYALPGQAISEHRSLAEVALRVPQGVVCLLSALRVHGIGTQAPFEVWLA
                     IEHRAPIPRLDQPKIRPVRMSGAAFTDGIEHLPVDGVSVPVFNAAKTVADCFKYRNKI
                     GLDVALEAMRDGWAQRKFTMDEIWHFATVDRVANVMRPYLESVTT"
     gene            complement(2315..2512)
                     /locus_tag="PLB25_17315"
     CDS             complement(2315..2512)
                     /locus_tag="PLB25_17315"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE43370.1"
                     /translation="MIHGEPGSGKSVVLRVLAQRLKRLPDVTVGAINHPQSHLADFYR
                     ELGDVFGVPLRPSNRWGGFKA"
     gene            complement(2671..3639)
                     /locus_tag="PLB25_17320"
                     /pseudo
     CDS             complement(2671..3639)
                     /locus_tag="PLB25_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            3711..>3924
                     /locus_tag="PLB25_17325"
     CDS             3711..>3924
                     /locus_tag="PLB25_17325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43371.1"
                     /translation="MARKSQRPALVLTQEQRSKLQELSASRVASTREVERAKVMLGYA
                     DGISITELQRKRVGKSPCGTLRFSRPS"
BASE COUNT          909 a         1092 c         1071 g          852 t
ORIGIN      
        1 atgtaaaacc cctactgtcg ccccggcaaa gccgggggat tacctgtttt attcaccaca
       61 tcggccagca cgattggatc aagctggttc tttttcagaa aagccttcca ctgagtttgc
      121 ttggttgcat cgtttgcgaa ggtatcggtg aacccaacag gcaccgtggt cggcatcggc
      181 attttccggc gttcaaacgt tgcttcaatg gcgcggcgga cttcggatgg gtcaagcgtg
      241 gtgtcatcca atagcaccca caaatcgaag taatccttca ttcgtgtgtt ggccaacccc
      301 agcagacaca ccgcatggaa tttttcggcg acgaccgtgt atttcggata ggcccgcaac
      361 tgcggtgccg gaaaatcttc caacagtacc ggatagctga tcgcctgtgg tgccggagtc
      421 accgcgtcgc caaacccgat atcaacttgc agtgcgatgc gcgcaccatc aagctttgcc
      481 tgaatgtcga tccggacccc accatagcct gcctctttgc ggatgatcga ccccttgact
      541 gaatcggggt caaacaccac accgtcttca gcaggcgtca ggcaaatttc ccgaaaagtc
      601 ctgaccgccg tgtcggcatc atccgggcca aatcccaaaa gatcagcgtc acgggtgggt
      661 cggtgtgcaa cgtcatacca aaacgtgaac agcagcgccc ctttgagcag gaaattcgag
      721 gcgtgctcgg aaacggacag tcggtacaac aaacgctcca gtccgtaatg cgtgagcgtc
      781 aaattgaagt cttgcttggt cgcatctgcg tgttgcttca gacgtgctcg gaccgatgcc
      841 gccagatttc gactcatgtt gtcacgcttt ccaggtatgg gcgcatgaca ttggccacgc
      901 ggtcaacggt cgcgaaatgc cagatttcgt ccattgtgaa ttttcgttgt gcccagccat
      961 cgcgcatggc ttccagggca acgtccagcc caattttgtt gcggtatttg aagcagtccg
     1021 ccacggtctt ggcagcgtta aataccggca cgctcacgcc gtcgaccggc aggtgttcga
     1081 tgccgtcggt aaacgcagcc cctgacatgc gcacggggcg aattttgggc tggtcaagcc
     1141 ttggaatggg tgcccgatgt tcaatggcaa gccagacctc aaaaggtgcc tgcgtcccga
     1201 tgccatgcac ccgcagtgcc gaaagtaggc aaaccacgcc ttgcggcact cgcagcgcca
     1261 cttctgccag tgaccggtgt tcgctgatgg cctgccccgg cagtgcataa acccctcgag
     1321 caactctttc gagctttcct gcggaaacca gtcggctgag cacaatggtt ggcagttctt
     1381 gctgcgcaag atcccgggca cgcagcagca gtcttaccct agcaagttcc aggacggcct
     1441 gctcttgggt ggaatgggaa cgtgggttca tgatgcgagt atgttggaat atataggcat
     1501 ttgtcaataa attgcatgga atagcaacaa acaagctcgc tcggcaaccg attcttactg
     1561 acctcagcag agttcaattt caatcgattt cgaacgccac ttgaccagca accgctccca
     1621 ggtgttagcg gtgcgttcac aatctcgtgc tgacgaatcg gactcggatc aacaatgcgt
     1681 tgccatcgtt ggtcatatcg cagttgctaa ataccgcatt ctggcagtag tcaccgcgct
     1741 gaaaaaacaa tgtcgatctt tagttccgca tgttggcgag gggttgtcca taatgaacaa
     1801 ttggacatat tctgcactac aaattcgaaa ggacttcgat atgggatggt ggggcgatca
     1861 atcgtaccag gctacgctac ctgcgaattc tgcggcggct ttggttgcac tggctgagca
     1921 ttactttgtg tcaatccagc agacaacaag tatagaaata cgcggcgatt tcgcgacaca
     1981 attcagcgtg gggaaaaaca tcatcagtgt ctggaggccc gaattgcatg agatgctggg
     2041 gatcgtgttc ccttgggcgt atcaatgtga aggcaccccg caaccgatat cattcacttt
     2101 ttgtcggcca aaaattgaca aatcaattga cgggtggcgt ctgatcaacc ccaagctcct
     2161 acagcccatc tcagattggt cagataaatc tattccctca aatcaaaatt tacatggccc
     2221 ttggacactt ttgtcactcg gaagtagatt tgaaattgac gggaccaaat acgaaagtgg
     2281 gtggccggag cgtggcaatc gggttgagtt agggctaagc cttgaatcca ccccaacgat
     2341 tgctgggacg cagcggcacg ccaaagacat caccgagctc acggtaaaag tcagccaaat
     2401 gactctgtgg gtgattgatg gcacccaccg tcacatcagg caaacgcttc aaacgctggg
     2461 ccaacacacg cagcacaaca ctcttgccgc tgcccggttc gccgtggatc atggcgaagc
     2521 cgccctcacg aatctgcgca tgctcaatac gccaacaaaa actctcaatc ttgggcggca
     2581 catagatcgc atccaccggc aactcaggcg agaacggatt gaatttgagc ccatacaggg
     2641 ccagcaattt ttggttcatt gaagtgcttc tcaggtgcgg ttatgggatg tgtcgggcaa
     2701 ggcgtttgat tcgggcatgg caagataggc cgggggcaca ccggtggcgg cgtaatcagc
     2761 caacagttgc gtcatcaaag ccggcagcgc cttgggtacc acaggcgaga gatcgacagc
     2821 cgccgggctc aaagcgcggc gctgaccact ggcattggct gacttatcca gtggcaagac
     2881 ctgacacagg gtggcacccg ttgtgccatc gaccaaatcc acccgcgaca aatcccagcg
     2941 ggcatagcgc aaattgactt gctccaggtg tcggtagcgc gacgcaattt caaagcgatt
     3001 gccagccaga ctcacggtgc cgtcggagcg cctctggcgg cgggccacct caatgcagaa
     3061 gctgttggcc agcacggcac tgcctggaca gggccttgcc acattcggtc cggccagata
     3121 atgcgccatg ggcgtggtat tgatctccga gtgaaaggtg cggtggtatt cctgctcaac
     3181 ccacgcctgc gtggccagat tcaacgtatc cagggtgagg cccgcatcgc cttcgagcat
     3241 ggccatcagc cgcccttcaa tacgccccca gaaggattcc tgtttggcgt tctgatatgg
     3301 cgaatacggc agcgtggtct ggtgcacgat gcccaaagcg gccaagccgt tggtggtttc
     3361 ttcagccgtc atggccgccc cgttgtcgct catcaaagcg cgtggcagac cgcgtttcat
     3421 aaaagcctgg cacaaagcat ggatcagact ttgggcagtc tcatcgagat accactgcag
     3481 gtgacacacc agacgggagc ggtcgttgat cacacacatg atcatggggg tcacccaggc
     3541 acctgagcgg gtcagcactt tgcgcgaacc gtggtggaaa tcgagatgcc aaagtgcgtt
     3601 gacgtggtca acttcaaagc tgcgcacctc tagtagttcg ttccatcaat acagttacta
     3661 actagttgat aatagccatc ctttcacttc aactgatgag ggtggtgatc atggcgagaa
     3721 aatctcaacg gcctgcgttg gtactgaccc aggagcaacg ctccaaactc caagagcttt
     3781 ccgcctcgcg ggtggcatct acgcgcgagg tggagcgcgc caaggtaatg ttgggctacg
     3841 ccgacggcat ctcgatcacc gagttgcaac gtaagcgggt gggcaagtca ccttgcggga
     3901 cgctgagatt ttcaagacca tcgt
//
LOCUS       DAOSLL010000479         3899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_20508_length_3899_cov_6.067898, whole genome shotgun sequence.
ACCESSION   DAOSLL010000479 DAOSLL010000000
VERSION     DAOSLL010000479.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3899
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20508_length_3899_cov_6.067898"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1376)
                     /gene="fliI"
                     /locus_tag="PLB25_17330"
     CDS             complement(<1..1376)
                     /gene="fliI"
                     /locus_tag="PLB25_17330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HOE43372.1"
                     /translation="MSTAELAPKWQVFLADAQKRVQTASPLAVRGTLTRLTGLVLEAV
                     GIRVPVGSQCWVTMKSQEPVLAEVVGFSNDRAFLMPAGDVHGLSSGASVEPAPAYVTA
                     PRLGERRHSGRTFGQGVLRLPLGNGLLGRVVDAHGAPMDYRGPILDVSSVPMDRKPIN
                     AMDRDPVREPLDTGIRAINALLTVGRGQRIGLFSGSGVGKSVLLGMMARYTVADVIVV
                     GLIGERGREVKEFIEDILGQEGRARSVVVAAPADAPPLLRMQGAAYATAVAESFRDKG
                     KHVLLLMDSLTRYAMAQREIALAIGEPPATKGYPPTCFAKLPQLVERSGNGLHGVGSI
                     TAFYTVLSEGDDQQDPIADAARAILDGHIVLSRTLAESGHFPAIDVEQSASRVMHNVV
                     TRDHFELARNFKAVYSRYEKGRDLIQIGAYVNGSDKYLDEAIALHEPMTRFLQQDMFE
                     AANMVSSVR"
     gene            complement(1363..2058)
                     /locus_tag="PLB25_17335"
     CDS             complement(1363..2058)
                     /locus_tag="PLB25_17335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HOE43373.1"
                     /translation="MRNYARFIPGEEIEAVEQWTFGAIDTAAQLLAAQVKAREAHAEE
                     VQDQLSRQESYKEGFAAGLVQGRAQAQAEVKIEMQKFLDTQAKDASAGLAELFASAQN
                     QLQEAEQAMAQGVLELSCELARQVLRQELSVNVQAVLPVLREALDLLGADCKTAVVRL
                     NPADLDALGEQIHLDFVGLALTLRADPTVLPGGCQVESAGMVVDGTLEKRWQRAVASL
                     GLSSVWEVSGEHG"
     gene            complement(2061..3059)
                     /gene="fliG"
                     /locus_tag="PLB25_17340"
     CDS             complement(2061..3059)
                     /gene="fliG"
                     /locus_tag="PLB25_17340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009433 - bacterial-type flagellum basal
                     body, C ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HOE43374.1"
                     /translation="MAQTDGLEDAAILMMSLGEAEASEVFKHLSPKEVQKLGEAIAKT
                     KTITRERVYEVVDKFTGIAAAQSLLVSNSGDYVRSVLKLALGEDKAALLIDRILQGGD
                     VSGIESLKWMEPSSVAELLRNEHPQIVAAILVHLDFDQAADILKFFNERQRNEVMLRV
                     ATMEGIQPSALKDLNEVLFKVLAGGDKMRKSSLGGIKTAAEMINLMGTAIEGTVIESI
                     RNHDPDLAQKIMDKMFVFDDVIKLDDKSIQTVLKEVSSDVLIVALKGAQPELKEKFLS
                     NMSTRAAESLREDLESRGPMRLSEVEAQQKEILKTVRRLADEGTIVIGGGADDAMV"
     gene            complement(3109..>3899)
                     /locus_tag="PLB25_17345"
     CDS             complement(3109..>3899)
                     /locus_tag="PLB25_17345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar M-ring protein FliF C-terminal
                     domain-containing protein"
                     /protein_id="HOE43375.1"
                     /translation="SQQLLESGTPGSSLPTGVPGATTNQPPGPSAAPVNGASQALTAA
                     GAASGVGNGRRESIINYEVDKTVRVVRGGTGIIKRISAAVVFNHQTTTDAKGKTTTAA
                     MSDAQLEKMTALVRETIGFNKDRGDSVNVVTTPFTLQKQAEVDVPVWQQPAVLDLARS
                     FAWPLGTLLFGALVLMGAVRPALKVMAQPMQIQETAAAGGLDAIESDQPDRPLLTGPS
                     SSQALPSEMSAGEVALEEARKLTRDNPAAVANIVKSWINGEAPA"
BASE COUNT          766 a         1308 c         1044 g          781 t
ORIGIN      
        1 cgcacgctgc tgaccatatt ggcagcttca aacatgtctt gctgcaaaaa ccgcgtcatt
       61 ggctcatgca acgcaattgc ctcatcgaga tacttgtcgg agccgttgac ataagcacca
      121 atctggatca gatcgcgccc tttttcatag cgcgagtaga ctgccttgaa attcctggcc
      181 agttcaaagt gatcgcgcgt cacaacgttg tgcatcaccc gcgatgccga ctgctcgaca
      241 tcaatggccg gaaagtggcc cgactctgcc agcgtgcgcg acagcacaat gtgcccgtcc
      301 agaatcgccc gcgccgcatc cgcaatcggg tcctgctggt catcaccctc ggacagcacg
      361 gtgtaaaagg cggtgattga cccaaccccg tgaaggccat tgccactgcg ctcgaccagt
      421 tgcggcagct tggcaaaaca cgtcggcgga taccctttgg tggctggtgg ctcgccaatc
      481 gccagggcga tttcgcgctg cgccatggcg tagcgtgtca gagaatccat cagcagcaag
      541 acatgcttgc ccttgtcacg aaagctctcg gctaccgctg tggcgtaggc tgcaccctgc
      601 atgcgcagca atggtggcgc atcggccggg gccgccacca ccaccgagcg ggcgcgccct
      661 tcctggccca gaatgtcttc gatgaattcc ttgacttcac gaccgcgctc gccaatcagg
      721 cccaccacaa tcacatccgc aacggtgtag cgcgccatca tgcccagcag cacgcttttg
      781 ccgacacccg aaccagagaa aagtccgatc cgctggccgc gcccgaccgt cagcaaggca
      841 ttgatggcgc gaatgccggt atcaagcggc tcgcgcaccg gatcccggtc catcgcattg
      901 atcggtttgc ggtccatcgg caccgatgac acgtccagaa tcggcccccg gtagtccatt
      961 ggcgcgccat gggcatccac cacccgtccc agcaggccgt tgcccaaggg caggcgcagc
     1021 acgccctggc caaaggtgcg cccgctgtgg cgtcgctcgc ccaggcgtgg cgccgtcacg
     1081 tacgccgggg ccggctccac gctggcaccg ctggacaggc catgcacatc gccggccggc
     1141 atcaaaaacg cccggtcgtt ggaaaaacca accacttccg ccagcaccgg ttcttgcgac
     1201 ttcatcgtca cccagcactg cgagccaacc ggcacccgga tgcccaccgc ctccagtacc
     1261 agcccggtca gccgtgtcag agtgccgcgc actgccagcg ggctggcggt ttgcacgcgc
     1321 ttttgcgcat cggccagaaa cacctgccat ttgggagcga gttcagccgt gctcaccgga
     1381 cacctcccag acactgctca aacccaaact cgccaccgca cgctgccagc gcttttccag
     1441 cgtgccgtcc accaccatgc cggcagactc aacctggcaa ccgccgggca gtaccgtcgg
     1501 gtcagcgcgc agagtcaaag ccaaaccgac aaaatcaagg tgaatctgtt cgcccagggc
     1561 gtccaggtct gccggattga gccttaccac agcggttttg caatcagcgc cgagcaagtc
     1621 caacgcctca cgcaggactg gcaacaccgc ctgcacattc accgacaact cctgacgcag
     1681 cacctggcgg gccagttcgc aggacagttc cagcacacct tgcgccatgg cctgctcggc
     1741 ttcttgcaac tggttttggg ctgaggcaaa cagttctgcc aagcctgcgc tggcatcttt
     1801 ggcctgggta tcgagaaact tctgcatttc aatcttgacc tcggcctgcg cctgcgcgcg
     1861 cccctgcacc aagcccgcag caaaaccttc cttgtaggac tcctggcggg acaattggtc
     1921 ttgcacttct tcagcatggg cttcacgcgc cttgacttgg gcggccagca attgggcggc
     1981 cgtgtcaatg gcaccaaagg tccactgctc gaccgcctca atctcttcac caggaataaa
     2041 acgcgcgtaa tttcgcatga tcaaaccatt gcatcatctg caccaccgcc gatcacgatg
     2101 gtgccctcgt cggccagacg ccgaacggtc ttgaggattt ccttttgttg cgcctcgacc
     2161 tctgacaggc gcatcgggcc gcgtgactcc agatcttcgc gcagcgactc cgcggcacgg
     2221 gtagacatgt tggacagaaa tttctccttg agttctggct gagcgccctt caaagccacg
     2281 atcagcacat ccgacgacac ttccttgagc acggtctgga tcgacttgtc gtcaagcttg
     2341 atgacatcgt caaagacaaa catcttgtcc atgatttttt gcgccagatc ggggtcatgg
     2401 ttgcgaatcg attcgatcac cgtgccttca atcgccgtgc ccatcaggtt gatcatttcc
     2461 gccgccgtct tgatgccgcc caatgatgac ttgcgcatct tgtcgccacc ggccaacacc
     2521 ttgaacagca cctcgttgag gtctttcagg gccgatggtt gaatgccttc catggtggcc
     2581 actcgcagca tcacttcgtt acgctggcgt tcattgaaaa atttcaaaat atcggccgcc
     2641 tggtcaaagt caaggtgcac cagaatggcc gccacaatct gtgggtgctc gttgcgcagc
     2701 aactcggcca cgctggacgg ctccatccac ttcaggctct ctatgcccga gacatcgcca
     2761 ccctgcaaga tgcggtcaat caacaaggcc gccttgtctt ctccaagcgc cagtttgagc
     2821 accgagcgca catagtcgcc cgaattcgac accagcaaac tttgcgctgc agcaatgccg
     2881 gtgaacttgt ccaccacctc gtaaacccgc tctcgcgtga tggttttggt tttggcgatc
     2941 gcctcaccga gcttttgcac ctctttgggc gacaaatgct tgaacacttc cgatgcctcg
     3001 gcttcaccca aagacatcat cagaatggca gcgtcttcca gtccgtcagt ttgtgccatg
     3061 attcatgcct taacgttcat cacccgatca aacatcgggc ccactgcgtc atgcaggtgc
     3121 ctcaccgttg atccagcttt tgacgatatt agcaactgca gcggggttgt cgcgggttaa
     3181 tttgcgggct tcttccaaag ccacctcacc ggcagacatt tctgaaggca gcgcttgcga
     3241 ggatgacggc ccggtcaaca gcggccggtc gggctgatcc gactcaatgg catcaagccc
     3301 gcctgcggct gccgtctcct ggatctgcat tggttgcgcc atcaccttca acgccgggcg
     3361 caccgcgccc atcagcacca atgcgccaaa cagcagggtg cccagcggcc aggcaaagct
     3421 gcgtgccaaa tccagcacag ccggctgttg ccacaccggc acatccactt cggcctgctt
     3481 ctgaagcgta aagggcgtgg tgaccacatt cacggaatca ccgcggtcct tgttgaaacc
     3541 aatcgtttca cgcaccaggg ccgtcatctt ctccaactgt gcgtcgctca tggctgcggt
     3601 ggtggtcttg cccttggcat cggtcgtggt ctgatggttg aacaccacgg cggcgctgat
     3661 gcgcttgatg atgccggtgc cgccgcgcac cacccgcacg gtcttgtcca cctcgtaatt
     3721 gatgatgctt tcgcgccgcc cattgccaac gccagatgcg gcaccggcag ccgtcagcgc
     3781 ctgtgaggcg ccattcaccg gtgctgccga cggacccggc ggctgattgg tggtagcacc
     3841 aggcacgccg gttggcaaac tgctgccggg ggtgccgctt tcgagcaact gctggctgc
//
LOCUS       DAOSLL010000480         3894 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_20551_length_3894_cov_5.523053, whole genome shotgun sequence.
ACCESSION   DAOSLL010000480 DAOSLL010000000
VERSION     DAOSLL010000480.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20551_length_3894_cov_5.523053"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="PLB25_17350"
     CDS             complement(<1..250)
                     /locus_tag="PLB25_17350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43376.1"
                     /translation="MGICSSPSWRTCVRTYKSWAQVERAFRSIKTMDLKLGPSHHHLE
                     GRVRSHIFLGMLAYYVEWRMRQVWRELMFADEDQAAKLV"
     gene            complement(495..2762)
                     /locus_tag="PLB25_17355"
     CDS             complement(495..2762)
                     /locus_tag="PLB25_17355"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HOE43377.1"
                     /translation="MKQLRSDLDVAALPNDALGCEIHDPKSGETYEFGAIEQFLIEQF
                     RHPYQIDEVCSKCNSQFGLQYTNEDISEFLGLLAKWKLLGEEENGPSDGRALSADDKE
                     DKSEKLSSEYTKYLDRPNRWHLFNPRSLLDGLNRLLQPVHFMIWLVPVVFTLGIITVM
                     HNFSAFVSDVSKVSSSFGFFGKLILSTFTVNIVSQAARGLVARYHGLAAPSLGIWLEL
                     GLIPRFNLQIIPKGRLTRRTRLWLSATPTLVRLWLFGVGILMWLLTRPSLTLSAIGAE
                     LAFVSFVGLAFSANPLWRGDGVNFLSALLDYPDLRERSHGAFWRLFVKRPAAIERHSR
                     RPIALGLFGLFSILFFLAVFGFIAYSVFHSLETRYQGVGAALFLLLSAYVSFTIRRNT
                     KAKKAQGKASSGKRFVASQTGSGSLSRWVKYSLLVIFIVCLFLPYRYETSGTAEVLPS
                     ARVTLSIEQPGVVDEVHFNGGEWVKAGTTLARLSYYKQFGELRATEAKIEAKRYEIAK
                     NQTTPSTEEIRLAENKILTVKMQARYSEEKLKRQEQLFLLGFVSQQSRDDTWNAAEHD
                     KKLLAEAEASLLALKAEVNPNQIKSLNAEMESLQHNANSFQEQVRRTYLISPIEGQIV
                     TKDLQFLSNSYMKEGAVFAQIENTRTVLVRVAVPESDSNGLSLGAELTLTLWAHPNRD
                     FKGTVTAIAPNVEEDSNKNRIVYVTSSVPNPDGALRSGLTGFAKVAQDRTIVLLAFTR
                     AFIRFVAVEAWSWIP"
     gene            3443..>3894
                     /locus_tag="PLB25_17360"
     CDS             3443..>3894
                     /locus_tag="PLB25_17360"
                     /inference="COORDINATES: protein motif:HMM:NF025126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOE43378.1"
                     /translation="MFSLAQGCIGWRCSGFAGVAELALPLAQVVLKLALLTIPGVQRL
                     VFEVMVSVQGRHPHGSERFTESGPLKADATPMQAMMRRLKTLARTAADAVHRQTLEPV
                     FGNIKLVMGFRQCSLCRLHKVTGEWHLVCLAWHVKRMAVPGSKIGQGK"
BASE COUNT          886 a         1067 c         1020 g          921 t
ORIGIN      
        1 gcaccagctt ggcggcttga tcctcatcgg caaacatgag ttcacgccaa acctgacgca
       61 tgcgccactc cacatagtag gccagcatgc caagaaaaat atgtgagcgc acccgacctt
      121 ccagatggtg atgacttgga cccaacttca agtccatcgt cttgatcgag cgaaatgccc
      181 gctccacctg tgcccaagac ttgtaggtgc gcacacaggt acgccaggac ggtgatgaac
      241 aaatgcccat ctgagcggtt tcctttttga tgaagaactg ggcgcaaacc cggttcacct
      301 ttcaagctgc gaaacacact ctcaatattt gtcagcgttg aataggactg ccacggtttc
      361 tgttcttgcc tggccagtag gttcgtgcgc aggcaattca ccccgacccc ggttgcgtac
      421 catggtgcct tggattcact ccttattcca gctcagcata cgtgctggtt catctggttt
      481 gacccgcaca ttggtcacgg aatccatgac caagcctcaa cagcaacaaa gcgaatgaat
      541 gctctcgtaa aggccaacag tacgatagtc ctatcttgcg ccaccttggc gaacccggtg
      601 aggccagatc gcaatgcccc gtccggattt ggcacactgc ttgtcacata cacgattcga
      661 ttcttgttgc tgtcctcttc tacgttcggc gctatcgcgg ttacagttcc cttgaaatcc
      721 cggtttggat gtgcccacaa cgtcagcgtc agttctgcgc cgagcgacaa accattgctg
      781 tcggattcgg gaaccgcgac cctcacaagc actgtgcgcg tattctcgat ttgggcgaaa
      841 accgcgccct ccttcatgta gctgttactc aggaactgga ggtcttttgt gacgatctgc
      901 ccttcaatgg gggatatcag ataagttcga cgcacctgct cctgaaagga attggcattg
      961 tgttgaagac tctccatctc cgcattcaga gacttaatct gattggggtt gacttccgcc
     1021 tttagtgcca gcaggcttgc ctcagcttcg gcaagcagtt tcttgtcatg ttcggcagca
     1081 ttccaggtat cgtcgcgact ctgttgcgag acaaatccca gcaggaaaag ttgttcctgg
     1141 cgcttgagtt tctcctcgct atacctggcc tgcattttga cggtaaggat cttgttttcg
     1201 gctaacctga tttcttccgt cgagggggtg gtttgattct ttgcgatctc gtatctcttt
     1261 gcctcgattt ttgcttccgt tgcgcgtaac tcaccaaact gcttgtaata ggaaagtcgt
     1321 gccagggtgg tgccggcctt cacccattcg ccgccattga aatgcacctc atcgacaaca
     1381 ccgggctgct caatggatag cgtgacccgt gccgatggca acacctcggc ggtgccactc
     1441 gtttcatatc gataaggcag aaaaaggcaa acgatgaaaa tgaccaacaa ggaatacttc
     1501 acccatcggc tcaacgagcc tgagccagtt tgcgatgcaa cgaaccgctt accagaagaa
     1561 gcttttccct gggctttttt ggccttggta ttgcgacgaa tagtgaaact cacataggca
     1621 cttaacagca gaaacagggc tgcaccaaca ccctgatacc tggtttccag agaatggaaa
     1681 acactgtagg caataaaccc aaataccgcc aggaaaaaca gtatcgagaa caatccaaac
     1741 aggccaagtg caatggggcg tcgggaatgt cgctcgattg cggcaggtct cttgacgaag
     1801 agcctccaaa atgccccatg cgaacgctcc cgcaaatcag gataatccaa tagtgccgac
     1861 aggaagttaa ctccatcgcc acgccaaagt ggattcgctg agaacgccaa gcctacgaat
     1921 gaaacaaagg caagttcggc accaatcgct gatagcgtca gcgacggtcg agttaatagc
     1981 cacatcagga tgcccacccc gaatagccac agccgcacga gcgtgggcgt tgcactcaac
     2041 catagccttg ttcggcgggt caaacggcct ttcgggatga tttgaagatt gaatcgtgga
     2101 atcaagccga gttccagcca tattcctaaa ctaggagcgg ccaaaccatg gtatcgggcc
     2161 accaaacccc gcgcagcctg cgaaacgata ttcacggtaa aagtagaaag gatcaacttg
     2221 ccgaaaaaac caaagctgga cgagactttg gatacgtccg aaacgaaagc actgaaattg
     2281 tgcatgacag tgatgatgcc gagagtaaag actaccggca ccagccagat catgaagtga
     2341 acgggttgaa gcagccggtt caatccatcg agcagtgacc gaggattgaa aaggtgccaa
     2401 cgatttggcc ggtccaggta tttcgtgtac tcagacgaca acttctcgga tttgtcctct
     2461 ttatcatccg cgctgagcgc gcgtccatcg gacggcccgt tttcttcttc tcctagcagt
     2521 ttccattttg caagaaggcc gagaaactca ctgatatcct cattggtgta ttgcagtcca
     2581 aactgactat tgcatttcga gcacacctca tcaatctggt atgggtgtcg gaactgttcg
     2641 atcagaaact gttcgatggc gccgaattcg taggtttcgc cactcttggg gtcgtgtatc
     2701 tcacacccca aagcatcatt gggcaaagcg gcaacgtcca agtcgctacg cagttgtttc
     2761 atttccgtta ccctttccaa ccattgatcc caaagtttct atttctcgcg cgccgcgatg
     2821 gccacctccc gcagggtatc aaaaacgcat ccgcaagttc ttcaatcgtc tcctgacaat
     2881 gtagttagcg actatacgtc cggcgcatcc gcaactgttg ctggcatact acatcactta
     2941 cattgagcat atgcgggcgc agcccttgac tgctctgtgt cggcccggtt gactccgtgg
     3001 atttgtccgc cagcgacagg gcgggtgtct tgcaacgacc actgtcgcgc tgccgacggt
     3061 ctggcgcatg ctgatttgac gatggtcttc acacagtggc ttttggctcg ccgaagtggg
     3121 gtgggcttca ttggtgacct tggcaacggg gggggggtag ctgatcggcg caagatcgtt
     3181 gtgctgctgt tgccaacagg ggtgcaacca gcgctgcagg gttcgacacg tcatgccaat
     3241 ttgcttgcag gcgcgctgca gccgtgaacc gtactggcag gcctgggtga tcaagctgat
     3301 ggtgtcttgg cgctgagcga ggctggtcat ttttcctccg cctcccaccg cgcctggcac
     3361 ttctttgcca catcaacggc gccgccgcat gcgccagtgc tttgtctttg cgacgaagtt
     3421 cgcgttctag tatttcgttc tgttgtttag cttggcgcag ggctgcatcg gctggcgttg
     3481 cagcggcttt gccggggtgg cagaactcgc gctgccactg gcgcaggtgg tgctcaaact
     3541 ggcccttctc acgataccag gcgtgcaacg cttggtcttt gaggtgatgg tttccgttca
     3601 gggcagacat ccgcacggga gtgagcgttt tacagagtct gggcccctga aggcagatgc
     3661 cacgccgatg caagcgatga tgcgccgctt gaaaacgctg gcacgcacag cggctgatgc
     3721 cgttcacagg caaacgttgg agccggtctt tggcaacatc aagttggtga tggggttcag
     3781 gcagtgctcc ttgtgtagat tgcacaaagt cactggcgaa tggcatctgg tttgcctggc
     3841 atggcatgtc aagcgcatgg cagtaccggg ttcaaaaatt ggacaaggaa aata
//
LOCUS       DAOSLL010000481         3880 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_20650_length_3880_cov_4.008627, whole genome shotgun sequence.
ACCESSION   DAOSLL010000481 DAOSLL010000000
VERSION     DAOSLL010000481.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3880
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20650_length_3880_cov_4.008627"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PLB25_17365"
     CDS             complement(<1..328)
                     /locus_tag="PLB25_17365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOE43379.1"
                     /translation="MSGITPAFSPLYQQIKGLILNSLRVGEWKPGEGIPSEMDLAARF
                     RVSQGTVRKAIDELAAENLLLRRQGKGTFVATHAEQQVQFRFLKLVPDSGNRGSEGPA
                     QRDIIEC"
     gene            578..1564
                     /locus_tag="PLB25_17370"
     CDS             578..1564
                     /locus_tag="PLB25_17370"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015913700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HOE43380.1"
                     /translation="MSKKPVRVAVTGAAGQIGYAILFRIASGEMLGKDQPVVLSLLEV
                     PVEKAQQALQGVMMELQDCAFPLVVGMEAHSDPMTAFKDVDYAMLIGSRPRGPGMERA
                     ELLAVNAEIFTSQGKALNAVASRGVRVLVVGNPANTNAYIAMKSAPDLPRKNFTAMLR
                     LDHNRALSQIASKIGKPVADIEKMAVWGNHSPTMYADYRFATVNGESVKDMINDHDWN
                     ANTFLPTVGKRGAAIIAARGVSSAASAANAAIDHMRDWALGTNGKWVTMGIPSDGQYG
                     IPKDTMFGFAVTCAGGEYKVVEGLTVDAFSQECINKTLKELQEEQAGVAHLL"
     gene            1589..2635
                     /locus_tag="PLB25_17375"
     CDS             1589..2635
                     /locus_tag="PLB25_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HOE43381.1"
                     /translation="MNHPRDVLLDATVAASLPVCDHYCGVEARMVKSLALQAEFIEEF
                     KTCVFDVTLDCEDGARVGGEKDHAFMVSALANHALEASNSVAKSVAQRIAVRVHPVSH
                     AAFEQDVEIIVGGAGAALCHLMLPKVESVADIDRALQAVDAASKGTRRPDSLPIHVLI
                     ESPLAVQRVAAIAAHPRVQSVSFGLMDFVSTHGGAIPASAMGVAAAGSGDGLDQFSHP
                     LVVRAKLEIAAACHASGKVPSHCVVTEFKDAAVLQAAARHASGALGFTRMWSIHPDQI
                     RPILAAFAPAEHDVAHASRIICEAQAVHWAPISVDGRLHDRASYRYYWQLLEKAHQTA
                     RLHRDDPAQRFFEM"
     gene            2672..3310
                     /locus_tag="PLB25_17380"
     CDS             2672..3310
                     /locus_tag="PLB25_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43382.1"
                     /translation="MMISGRHRHPYGFGKRMLVLGAIVIGSTWQMANNAVADEVIKKP
                     VKAVKPVSVSRAELRTNANQIATGVRAAEAALSPEELVIAQRVEVGNVACELGVSVSV
                     KADAKLPGYFDVQSRQYKFRMVPVVTSTGAIRLEDAHAGAVWLQLSNKSMLMSQKSGT
                     RLADVCMTPGQFAVAQAIEKNPPVSLLDPVRVSKQPVVEPVKPAVAPTVEPK"
     gene            3368..>3880
                     /locus_tag="PLB25_17385"
     CDS             3368..>3880
                     /locus_tag="PLB25_17385"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460204.1"
                     /note="catalyzes the conversion of citrate to isocitrate
                     and the conversion of 2-methylaconitate to
                     2-methylisocitrate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase B"
                     /protein_id="HOE43383.1"
                     /translation="MLQAYRAHVAERADLGIPALPLSAGQTAELIELLKKPPQGEEQF
                     LLELVTNRVPAGVDDAAKVKASYLAAVAHGTEACPLISRGKATELLGTMLGGYNLTPL
                     IDLLDDVAVADVAAEALKKTLLMFDQFHDVKEKADKGNAQAKTVLQSWADAEWFTSRP
                     EVPKSIKLTVL"
BASE COUNT          814 a          993 c         1151 g          922 t
ORIGIN      
        1 gacactcaat gatgtcgcgc tgcgctggcc cttcgctgcc acggttacca ctgtcgggga
       61 ccagtttcag aaacctgaac tgcacctgct gctcggcgtg cgtggccaca aaggtgccct
      121 tgccttggcg gcgcagcagc agattctcgg cggctaactc gtcgattgct ttgcgcacag
      181 ttccctgact aaccctgaaa cgcgcggcca aatccatctc gctcggaatg ccttcccccg
      241 gcttccactc tccgacgcgc agactgttga ggatcaaacc tttgatttgt tggtacagag
      301 ggctgaacgc gggtgtgatc ccggacatcg ctgctgtttc agggatggtc gggtcattat
      361 ccggggcggc ttcaggcgtg atctgcatgg agaaaggcct ttgtaagaat gttatgttca
      421 tacgaatcat atcttatata agatataaga caagaggacg atgccgcatt cttggtggat
      481 ttggagagta cacttgctcc cgctcggagc tgatgccttt ggtttatcct ccccgcaccc
      541 gctgaatatc tatcaataaa tttctggagt tttcatcatg agcaaaaagc ccgtccgcgt
      601 tgctgttacc ggcgctgcag gtcaaattgg ttacgccatt ctgtttcgta tcgcttcggg
      661 cgaaatgctc ggcaaggatc agcctgttgt cctgagtttg ctggaggtac ccgttgaaaa
      721 agcgcagcag gccttgcaag gtgtgatgat ggaactgcaa gattgtgcat ttcctctggt
      781 ggtgggtatg gaagcgcaca gcgatccgat gaccgcgttc aaagatgtcg attacgcgat
      841 gttgatcggc tcacgtccgc gcggccccgg catggaacgc gctgagttgc tggcagtgaa
      901 cgctgaaatt ttcacttctc agggcaaggc attgaatgcg gtcgcgagcc gtggcgttcg
      961 tgttctggtg gtgggaaatc ccgccaacac caacgcttac attgcaatga agagcgcccc
     1021 cgacctgcct cgcaagaatt tcaccgccat gctgcggctg gatcacaacc gggcgttgag
     1081 ccaaattgcg tccaaaatcg gcaagccagt cgctgatatc gagaagatgg ccgtttgggg
     1141 caatcattcg cccaccatgt atgcagacta tcggtttgcc actgtcaatg gcgaaagtgt
     1201 caaggacatg atcaacgatc atgactggaa tgccaacact ttcttgccca ccgttggcaa
     1261 gcgcggcgct gccatcattg ccgcacgtgg cgtgtcttcg gctgcctctg ctgccaatgc
     1321 cgccattgac cacatgcgcg attgggcgct tggcacgaac ggcaagtggg tcaccatggg
     1381 tatcccctca gacggtcaat acggcattcc aaaagacacc atgtttggtt tcgccgtgac
     1441 ttgtgcgggt ggcgagtaca aggtcgttga aggtttgacc gtcgacgcat ttagtcaaga
     1501 atgcattaac aagacgctca aggaattgca ggaagagcaa gcgggtgtgg cccatcttct
     1561 gtaaggtttt gctggaggag tcaggctcat gaaccacccg cgggatgttt tactggatgc
     1621 cactgtggcg gcgtcattgc cggtgtgtga tcactactgt ggtgttgagg ctcggatggt
     1681 caagagtctg gccttgcagg ctgagttcat tgaagagttc aagacctgcg tttttgatgt
     1741 cacgttggac tgcgaagatg gcgcgcgcgt tggcggtgaa aaagaccatg cttttatggt
     1801 ctcagccctt gccaatcatg cgctggaagc ttctaattct gtagcaaaat cggttgctca
     1861 gcgaattgcc gtgcgggttc acccggtgag tcacgctgct ttcgagcagg atgtcgaaat
     1921 cattgtgggt ggtgcaggcg cggctttgtg ccatttgatg ttgcccaagg ttgagtccgt
     1981 cgcggatatt gaccgagcgc ttcaggctgt ggacgcggcc agcaagggca cgcgacggcc
     2041 cgattctttg ccaatccatg tcctgattga gtcgccgctg gcggtgcagc gtgttgctgc
     2101 cattgctgcg cacccgcgtg tccaatctgt cagtttcggt ttgatggatt ttgtatcaac
     2161 ccatggtggt gctattccgg caagtgccat gggtgtcgcc gcagcgggtt cgggtgatgg
     2221 tctggatcaa ttcagccatc cgctggtggt tcgtgccaag cttgaaattg ccgcagcgtg
     2281 ccatgcgtcc ggcaaggtgc cgtcacactg tgtggtgaca gagttcaagg atgccgcagt
     2341 gttgcaagcc gccgcccggc acgcatccgg tgcgctgggc tttacacgga tgtggagcat
     2401 tcatcctgat caaatccgcc ctattctggc ggcctttgca cccgccgagc acgatgtcgc
     2461 tcatgccagt cgtatcattt gcgaagcgca ggcagtgcat tgggcaccta tctcagttga
     2521 tgggcgttta catgaccggg ccagttaccg ctactactgg caattgcttg agaaagcgca
     2581 tcagactgcc cgcttgcacc gtgacgatcc ggcgcagcgc tttttcgaaa tgtgaattgt
     2641 gactgatgat ttgtatatac aggagtacca gatgatgatt tcaggccgcc acaggcatcc
     2701 gtatggcttc ggcaaacgaa tgctggtcct aggggcgatt gtgataggtt caacttggca
     2761 gatggcgaac aacgccgttg ctgatgaggt gattaagaag ccggtcaagg cggtcaagcc
     2821 ggtctcggtc agtcgtgcgg aactcagaac caacgcgaac cagattgcta ccggcgtacg
     2881 ggctgcggaa gcggccctga gtcccgaaga actggtgatc gcccaacggg tggaagttgg
     2941 taatgtggcc tgtgagttgg gtgtttccgt cagcgtcaag gcggatgcga aattgccggg
     3001 ttactttgat gtccagagca ggcagtataa gtttcgcatg gttccggtgg tcaccagcac
     3061 gggtgccatt cggctggagg atgcgcatgc cggcgctgtg tggcttcagt tgtcaaataa
     3121 atcgatgctg atgagccaga aatcgggaac acgtttggct gatgtctgca tgactccagg
     3181 gcagtttgct gtggcccagg caatcgaaaa gaatcccccg gtcagtttgt tggaccctgt
     3241 ccgagtgtca aaacagcccg tggtggaacc cgtcaagcct gctgtcgcgc cgacggtcga
     3301 accaaagtaa gaacgatctg taaccatgga ttttcattag taagagttga ttgaaaagga
     3361 gtttctcatg ttgcaagctt atcgcgctca tgttgccgag cgcgccgacc tcggcattcc
     3421 ggcgttgccg ctgagcgcag ggcagaccgc cgagttgatc gagttgctga aaaagccacc
     3481 gcaaggtgaa gagcagtttt tgcttgaact ggtcaccaac cgtgtacctg ccggtgtcga
     3541 tgatgctgca aaagtcaaag ccagctatct ggctgcggtg gcacacggca ctgaagcctg
     3601 tccgctcatc agccgcggaa aagccacgga gttgctgggt acgatgttgg gcggatacaa
     3661 cttgacccca ttgattgatc tgctcgatga cgtagcggtc gccgacgtag cagccgaagc
     3721 cctcaagaag accttgctga tgtttgacca gtttcatgat gtcaaggaaa aggctgacaa
     3781 aggcaatgca caagccaaga ctgttctcca aagttgggct gatgccgagt ggtttaccag
     3841 ccgtcccgaa gtaccgaaaa gcattaagct gacggtgttg
//
LOCUS       DAOSLL010000482         3842 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_20887_length_3842_cov_4.869026, whole genome shotgun sequence.
ACCESSION   DAOSLL010000482 DAOSLL010000000
VERSION     DAOSLL010000482.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20887_length_3842_cov_4.869026"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="PLB25_17390"
     CDS             complement(<1..723)
                     /locus_tag="PLB25_17390"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HOE43384.1"
                     /translation="MPNAPKAPLRQLGGSDAAFIYSDTEHANSNVSLLHIYDQTTAPN
                     GVVRFKQILTHIEARLHQLPMFRQKVLRVPLDLDYPYWVEDDNFDIEYHVRHIALPKP
                     GDWRQFCIQASRIHARPLDLQRPLWEMYVIEGLDSFLDLPAGSFAVLLKVHHAAVDVA
                     HEHEITSLLHDTSPNPPPEAPVRPWFPPPPPGPLALLWRAGFRAASSPLRMAGSVSEG
                     LGMMALTVKTVAGEFLLHPNDFP"
     gene            complement(727..1551)
                     /locus_tag="PLB25_17395"
     CDS             complement(727..1551)
                     /locus_tag="PLB25_17395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009857616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HOE43385.1"
                     /translation="MLSKSIVSRHHHVGAPHLLHSLLEPRAFLEAAMLPMSLPLLMQA
                     PQGDGHPVLLLPGFMADEKSLFVLKAFLERKGYNVQTWGLGRNLGFRSKHASALPQKI
                     RYLHHLTGRKVSLVGWSLGGVFSLYGAENSLECVRCVITLGSPVSMDAQGSQSPAAVK
                     ALYRLVSHRLGSSAHAMAPRAKALREHRRLAIPTSCLYSLGDGVVPPQEATIDGDPAL
                     HENIRVPGSHIGLGFNGLVLAVVADRLAQPEAQWTPFAPKGLLAQVWRMMPKDKAD"
     gene            complement(1569..3089)
                     /locus_tag="PLB25_17400"
     CDS             complement(1569..3089)
                     /locus_tag="PLB25_17400"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HOE43386.1"
                     /translation="MRELTSDIESLSGLDGAFLNLETMATPMHVGSLHTFEVPKDYQG
                     DFFTDVKTLLTQRMMPLLRRRLASLPLQMANPVWVQGEVDMDQHVERIRIPAPGLRAQ
                     LEVVVGNLHADLLDRSRPLWMMYVLDGLASGERAYYFKIHHAMLDGHAGAALAATLFD
                     TAPHALTAASATAPLGRAASSKPAALSLVAAAFRHDATQYVKLVRDLPGVLKTLVGLV
                     RSSGGGHAPAGPDKANRPAKNGMALGPRTPLNVAISRERGFAAESIPLAEVKAIAKAC
                     EATVNDVVLALCGGALRRYLARLGSLPKRSLIASMPISLRDKGDTGFHTKATLSLVSL
                     ATHIKDPLKRLQAVRGNTSATKAVARRAKAVIPTDFPSIGVPWLVGALANLYGRGGIS
                     EVLPPLANLLISNVPGPAVPLYVGPARMTGYWPLSIVEHGVGLNITLMSYAGQLCLGF
                     TVARCAVPDVRLLADDFVQAFDELRQAGLAAAKPAGTVKAAVRRVPVKARAKSVTQ"
     gene            3515..>3842
                     /locus_tag="PLB25_17405"
     CDS             3515..>3842
                     /locus_tag="PLB25_17405"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HOE43387.1"
                     /translation="MNTTQIKPGVATGDSLQALFDLARAKNFAIPAVNVINTSTVNAV
                     IETAKELNSPAIIQFSNGGAQFYAGKGLDNAGQRACIAGAVSGAHHVHQMAELYGATV
                     VMHTDHA"
BASE COUNT          819 a         1190 c         1141 g          692 t
ORIGIN      
        1 cgggaagtca tttgggtgga gcaaaaactc gccagcaacg gtcttgacgg tcaaagccat
       61 catgcccaag ccttcggaca ccgacccggc catgcgcagc ggagagctgg ccgcgcgaaa
      121 accagcccgc cacagcagcg ccagcgggcc cggcggcggc ggcgggaacc agggtcgcac
      181 cggtgcttca ggcggtgggt tggggctggt atcgtgcagc aggctggtga tttcgtgctc
      241 atgtgccacg tccaccgcag catggtgcac cttgagcagc acggcaaagc tgcccgctgg
      301 caaatccaga aagctgtcaa gcccctcgat cacatacatc tcccacaacg ggcgctgtag
      361 atccagtggc cgtgcgtgaa tgcgcgaggc ctgaatgcaa aactggcgcc agtcacccgg
      421 cttgggcagc gcaatgtggc gcacgtggta ttcgatgtcg aagttatcgt cttccaccca
      481 ataggggtag tccaggtcca gcggcacccg cagtactttt tgacggaaca ttggcaactg
      541 gtgcagccgg gcctcaatgt gggtcaggat ttgcttgaaa cgcacgacac cgttcggggc
      601 cgtggtctgg tcataaatgt gcagcagcga cacattcgaa ttggcatgtt cggtatcgga
      661 gtaaatgaaa gcggcatcgc ttccaccgag ctggcgcagc ggggctttgg gcgcgtttgg
      721 catggttcag tcggccttgt ctttgggcat catgcgccag acctgtgcca gcaaaccctt
      781 gggcgcaaag ggtgtccact gcgcctccgg ctgtgccagc cggtctgcca ccaccgccag
      841 caccagccca ttgaaaccca gcccgatgtg gctgcccggc acgcgaatgt tttcatgcag
      901 cgccgggtcg ccgtcaatgg tggcctcttg tggtggcacc acgccgtcgc cgagcgaata
      961 caggcaactg gtcggaatcg ccagacggcg gtgttcgcgc agcgccttgg cacgcggtgc
     1021 catggcgtgc gcgctggagc ccaggcgatg cgacaccagc cggtacaagg ctttcacagc
     1081 ggcaggtgac tggctgcctt gcgcatccat gctgaccgga ctgcccaggg tgatgacgca
     1141 acgcacacat tccagcgaat tctcggcccc gtaaagcgaa aacacgccgc ccagactcca
     1201 gccaaccaag ctcaccttgc ggccggtcag gtgatgcagg tagcgtattt tttgtggcag
     1261 ggcactggcg tgcttgctgc gaaaacccag gttgcgcccg agcccccagg tttgcacgtt
     1321 gtatcccttg cgctccagaa aggccttcag cacaaacagg gacttttcgt ctgccatgaa
     1381 accgggcagc aacagcaccg gatggccatc accctgcgga gcctgcatca aaagcggcag
     1441 cgacatcggc aacatcgccg cctcaagaaa agcccgcggc tccagcaagg aatgcaataa
     1501 atgcggcgcg ccaacatggt gatggcggga aacgatggat ttggacagca tggtgtgcag
     1561 gctccgggct actgggtcac tgacttggcc cttgctttaa caggaactcg gcggactgcg
     1621 gccttgacgg tgccagcggg cttggcagca gccaggcctg cttgcctgag ttcgtcaaac
     1681 gcctgtacaa aatcatcggc cagcaggcgc acgtcgggca cggcacagcg cgccacggta
     1741 aagcccaggc agagctggcc ggcataactc atcagcgtga tattcagccc gacaccgtgt
     1801 tcgacgatgg acagcggcca gtagccggtc atgcgcgccg gacccacata aagcggcacg
     1861 gcaggccccg gcacattgga aatcagcagg ttggccagcg gcggcaacac ctcggaaatg
     1921 ccgccgcgcc cgtacaagtt ggccagggca ccgaccagcc agggcacgcc aatcgatgga
     1981 aaatcggtcg gaatcaccgc cttggcacgt ctggccacgg ctttggtggc gctggtgttg
     2041 ccgcgcaccg cctgcagacg cttgagcggg tctttgatat gggtggctag gctgaccagg
     2101 cttaacgtgg ccttggtgtg aaagccggtg tcacccttgt cacgcagcga gatcggcatc
     2161 gaggcaatca gcgacctctt cggcagcgag cccaaacgcg ccaggtagcg gcgcaaggca
     2221 ccgccgcaca aggccagcac gacatcattg acggtggcct cacaggcttt ggcaatggcc
     2281 ttgacctcag ccagcggtat cgactcagcg gcaaagccac gttcacggct gatggcgaca
     2341 ttgagagggg tgcgcggccc caatgccatg ccgttttttg ctggccgatt ggccttgtcg
     2401 ggaccagcag gcgcatggcc accacccgaa ctgcgcacca ggccgaccag cgtcttgagc
     2461 acacccggca gatcacgcac cagcttgaca tactgggtgg catcatgacg aaaagctgcc
     2521 gccaccagac tgagcgcagc gggtttgctg ctggcagcgc gccccaacgg tgctgttgca
     2581 ctggccgctg tcaacgcatg cggcgcggtg tcaaacaggg tggccgccag cgcagcaccg
     2641 gcatggccat caagcatggc gtgatgaatc ttgaagtaat aggcccgctc gccactggcc
     2701 agaccgtcta gcacatacat catccacagt ggccgggagc gatccagcaa atcagcatgc
     2761 aggttgccca ccaccacctc aagctgcgcg cgcaggccgg gtgccgggat gcggatgcgt
     2821 tcaacatgct gatccatgtc aacctcgcct tgcacccata ccggattggc catttgcagc
     2881 ggcaggctcg ccagacgcct gcgcagcagc ggcatcatgc gctgcgtcag caaggtcttg
     2941 acatcggtaa aaaagtcgcc ctggtagtct ttgggaacct caaaggtatg cagtgaaccc
     3001 acatgcatgg gcgtggccat ggtctcaaga ttcagaaaag ccccatccag accactcagg
     3061 gattcgatgt cagaagtcaa ttcgcgcata acggtcggcc cggttttttg ctggtgcaaa
     3121 gtgtcgtttc attttacggc cgcctgtttt accggtgact gcggcgccgt cgccttggtt
     3181 acagaggagc ttccaaatga ttcaaattac tatgattagt gtagctatca gcgcatgctg
     3241 caaaagggct gaaagcctat ttgccttgca aagccgggca tatttgggtg accactccaa
     3301 tcaccagaca tcagacccgg cggtgcctga aagcgtccct gctgccccca tcagatggca
     3361 gacacgcaca ccggatgcca gcgcgagcca gtgtgccatc gggtcgtcgg cgtgatgccg
     3421 tcaaatcagt cgctaaaatg accgcggcac tgcgcaatga ttgccatccc accacccagt
     3481 ccattcaatc gacctcaaca ggaccccaaa aaatatgaac actacccaaa tcaaacccgg
     3541 tgtcgcaaca ggagacagcc tgcaagccct ttttgacctg gcccgcgcga agaactttgc
     3601 catcccggcc gtcaacgtca tcaacaccag caccgtgaat gccgtcatcg agaccgccaa
     3661 agagttgaat tcaccagcaa tcatccagtt ttccaatggt ggtgcacagt tttatgctgg
     3721 caaagggctt gacaacgcag gtcagcgcgc ctgcattgcc ggtgctgttt ccggtgccca
     3781 ccatgtgcat caaatggccg agttgtatgg tgccaccgtg gtcatgcaca ccgaccatgc
     3841 cg
//
LOCUS       DAOSLL010000483         3833 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_20954_length_3833_cov_4.005823, whole genome shotgun sequence.
ACCESSION   DAOSLL010000483 DAOSLL010000000
VERSION     DAOSLL010000483.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20954_length_3833_cov_4.005823"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="PLB25_17410"
     CDS             <1..253
                     /locus_tag="PLB25_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HOE43388.1"
                     /translation="DAACNAVVSSFYTRLGQHGWLFLIFACVFMAAMSVVGDLIESLV
                     KRSAGVKDSSGLLPGHGGVLDRIDALLPALPLAMLLYSY"
     gene            262..1467
                     /gene="dxr"
                     /locus_tag="PLB25_17415"
     CDS             262..1467
                     /gene="dxr"
                     /locus_tag="PLB25_17415"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HOE43389.1"
                     /translation="MTDKNRRQRVAILGSTGSIGVSTLEVIARHPQRFEVFALSAATQ
                     VDKMLTQCAQFKPVFAVMASIEQGRLLQQKCEENGLPTHILYGPEAIETIASDERVDV
                     VMAAIVGAAGLASCLAAARAGKRLLLANKEALVVGGDYFLQAVATGGAKLLPIDSEHS
                     AVFQSLPDDLRMWSEQIDKIILTASGGPFRRRDPATFGGITPEQACAHPNWVMGRKIS
                     VDSATMMNKALEVIEARYLFGVQPGQIEVVIHPQSVIHSMVQYRDHSIVAQLGTPDMK
                     GPIAYGLSWPERIESGASVLDFGSMVDLSFESTGSNGHAERFPGLKLAWSVLHAPPGS
                     TAVLNAANEIAVAGFLAGKIRFDQIHAVNLETLSGVVTSPPGSLDDLLALDGQSRQAA
                     QAVVARIGR"
     gene            1489..2841
                     /gene="rseP"
                     /locus_tag="PLB25_17420"
     CDS             1489..2841
                     /gene="rseP"
                     /locus_tag="PLB25_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HOE43390.1"
                     /translation="MLTLPAFILAIALLIAVHEYGHYRMALACGVKVLRFSIGFGKPL
                     LRWQGQSTEFVLAIFPLGGYVKMLDEREAPVDDAERHLAFNTQPLRSRVLIVAAGPVA
                     NLLLAVVLYSAVNWIGVELPVAVLAQPAAGTIANQAGLSGGDRVLRVALAGDTPKEVA
                     SFDDVRWALTRAALQGEDVLIQVARGGQTEPDIRLKLSALEATEVDAEMFARIGIIGP
                     FTRPMIGDITTDGAAAQAGLLKGDEVLAVNDQTIVDGPQLRQVIRSSGATGQVSPQRW
                     KVSRQGQSLEINLTPLVVSEGDKTVGRIGAYVGAAPDMVLVRYKLIDGLSRGARQAWD
                     VSILTLKMMGKMLIGQASVKNLSGPLTIADYAGKSAGLGLTQYLIFLALISVSLGVLN
                     LLPLPVLDGGHLMYYLWEGITGKPVPDHWMEHLQQGGIALLTIMMAIAVFNDITRLFG
                     "
     gene            2886..>3833
                     /gene="bamA"
                     /locus_tag="PLB25_17425"
     CDS             2886..>3833
                     /gene="bamA"
                     /locus_tag="PLB25_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOE43391.1"
                     /translation="MHFNRFRLSMLSALVSMVMVSQSAWAVEPFKVRDIRIEGLQRVE
                     PGTIFVSLPVQVGDTYTDDKGSAAIRTLFGLGLFKDVRIEVNGDVLVVVVEERPTVAD
                     IDFAGIKEFDKDVLIKSLRDVGLADGRPFDKAQLDRAEQELKRQYINRSLYAAQVVTT
                     VTPIERNRVNLTFTVTEGEPAQISEIRIVGNKVFTESSLRDLFDLDTGGWLSWYTKSN
                     RYSRAKLNADIETLRSYYLTRGYLEFKVDSTQVAIAPNKTDISITINVTEGERFIVTS
                     VKLAGDFLGKDDEFKTLVAIRPGQAYNADEVAKTTKAFTD"
BASE COUNT          766 a         1009 c         1149 g          909 t
ORIGIN      
        1 cgatgccgcc tgcaacgcgg tggtgtcaag cttctatacc cgtctgggcc agcatggctg
       61 gcttttcttg atctttgcct gcgtcttcat ggctgccatg agtgtggtgg gtgatttgat
      121 tgaatcgctg gtcaagcgaa gtgccggggt gaaggacagc agcgggttgt tacctggcca
      181 tggcggcgta cttgatcgca ttgatgcact gctgcccgca ttgccgttgg cgatgctgct
      241 gtattcctac tgagaatgcc catgactgac aagaatcgca ggcagcgcgt cgcaatcctg
      301 ggctcaaccg gctccattgg cgtcagcact ctggaggtca tcgccaggca tccacagcga
      361 tttgaagttt ttgccttgag tgccgcaaca caggttgaca aaatgttgac gcagtgcgca
      421 cagttcaagc cggtctttgc cgtcatggcc agtattgaac agggtcggct tctgcagcaa
      481 aagtgtgaag aaaatggctt gccaacccat attctgtatg gccctgaagc tattgaaaca
      541 atagcgtctg atgagcgagt tgatgttgtc atggcggcca ttgtcggggc tgccggtttg
      601 gcttcctgcc tggctgcggc ccgtgctggc aagcgcctgc tgctggccaa caaggaggca
      661 ctggtggtgg ggggcgacta tttcctgcaa gcggttgcta ccggtggtgc caaactgctg
      721 cccattgaca gtgagcattc ggcggtgttt cagtccttgc cggacgattt gcgaatgtgg
      781 tcggagcaaa ttgacaagat catcttgacg gcatcggggg gcccgtttcg ccgacgagac
      841 cctgccacct ttggtggcat cacgccagag caggcctgcg cgcatccgaa ttgggtgatg
      901 gggcgaaaga tctcggtgga ctctgccacc atgatgaaca aggcactcga agtgattgag
      961 gctcgctacc tgtttggcgt gcaaccgggg caaatcgagg tggtgatcca cccacaaagc
     1021 gtcatccatt ccatggtgca gtaccgcgac cattcgatcg tggctcaact tggtacgcct
     1081 gacatgaagg ggccaattgc ttacggcctt tcctggcccg agagaattga gtcaggtgct
     1141 tcggtgctgg acttcggctc catggttgac ctgagcttcg aatcaacagg gtcaaatggt
     1201 catgccgagc gctttcccgg tttgaaactg gcctggtcgg tgttgcacgc accgcccggc
     1261 agcaccgccg tgctgaacgc cgcgaacgaa attgcggtgg ctggctttct ggccggcaag
     1321 attcgattcg accagattca cgcggtgaac ctggaaaccc tgtccggtgt ggtgacatcg
     1381 ccaccgggtt cacttgacga cttgctggca ctggatggtc agtccaggca ggcggcgcaa
     1441 gcagtggttg cgcgcatcgg ccgttgacat cgaccccacg cttaagccat gttgaccttg
     1501 cctgcgttca tcctggccat tgcactgctg attgcggttc acgagtacgg gcattaccgc
     1561 atggctttgg cctgtggtgt caaggtactg aggttttcca tcggttttgg caaaccgctg
     1621 ctgcgctggc agggtcaatc caccgagttt gtgctggcca tttttccttt gggtggctat
     1681 gtgaagatgc tcgatgagcg cgaggccccg gtggatgacg cagaacgtca tctggccttc
     1741 aatacccagc ccctgcgcag ccgcgtgctg attgtggccg ccggtccggt ggccaacctg
     1801 ctgctggcgg tggtgcttta cagtgcggtc aattggatcg gtgtggaact cccggttgct
     1861 gtactggcgc agcctgcagc cggaacgatc gccaatcagg ctggcctgtc tggcggtgac
     1921 cgcgttctgc gcgtcgcact ggctggcgat acgccaaaag aggtagcgtc ttttgacgat
     1981 gtacgctggg ccctgacccg tgctgcattg cagggtgagg atgtgctaat tcaggttgcc
     2041 aggggaggcc agactgagcc ggatatccgg ttgaaactgt cggcacttga agccaccgag
     2101 gtggatgcag aaatgttcgc cagaatcggc attattggcc cgtttaccag acccatgatt
     2161 ggcgacatca cgaccgacgg tgcggctgcg caggcgggtt tgttgaaagg tgacgaggtg
     2221 ctggccgtca atgaccaaac catcgtcgat gggccacaac tcaggcaggt aatccgcagt
     2281 tctggtgcca ctggccaggt aagcccccag cgttggaagg tatcccgcca ggggcaaagc
     2341 ctcgagatca acttgacccc cctggtggtg tctgaaggcg acaaaaccgt tggccgcatt
     2401 ggtgcctacg taggggcggc acctgacatg gtgctggtac gttacaagct gattgacggt
     2461 ttgtcgcgcg gcgctcgaca agcctgggat gtttccatcc tgacacttaa aatgatgggg
     2521 aaaatgctga ttggtcaggc atcggtcaaa aatctcagcg ggccactgac catcgcagac
     2581 tatgcgggta aatcggctgg gctggggtta acccaatatc tgatttttct cgcgttgatc
     2641 agcgtcagtc taggcgtttt gaacctgttg ccgttgcccg ttcttgatgg cggtcacctg
     2701 atgtattatc tttgggaggg cattaccggc aagccggtgc cagatcactg gatggaacat
     2761 ctgcagcagg gtggtattgc tctcttgacg atcatgatgg caattgccgt cttcaacgat
     2821 atcacccgct tgtttggtta gctcacatcg tcttttcgac accacgtcaa atattgttta
     2881 aacaaatgca tttcaatcgt tttcgcctga gcatgctttc tgctttggtc tcaatggtca
     2941 tggtgtctca atcggcctgg gcggtagagc ctttcaaggt gcgagacatt cgcatcgaag
     3001 gcctgcaacg ggtcgagcca ggtaccattt ttgtgtcttt gccagttcag gttggcgaca
     3061 cctacaccga tgacaagggc tccgctgcca ttcggacttt gttcggactg ggcttgttca
     3121 aggatgtacg cattgaggtc aatggtgatg tgctggtggt ggtggttgaa gagcggccaa
     3181 ccgtggctga cattgacttt gccggcatca aggaatttga caaggacgtg ttgatcaaat
     3241 ccctgcgtga tgtcggattg gccgacggca ggccgtttga caaagcccag ttggatcgcg
     3301 ccgaacagga actcaagcgc caatacatca accgcagtct ttatgcggcc caggtggtca
     3361 ccaccgtgac cccgatcgag cgcaaccgcg tcaacctgac ttttaccgtg acagaaggtg
     3421 agcctgccca gatcagtgaa atccggattg ttggcaacaa ggtgttcacc gagtcaagct
     3481 tgcgcgattt gtttgatctg gacacgggtg gctggctaag ctggtacacc aagtccaacc
     3541 gctattccag ggccaaactc aatgctgaca ttgaaaccct gcgctcgtat tacttgaccc
     3601 ggggttatct ggagttcaag gtggattcca cccaggtcgc cattgccccc aataagaccg
     3661 acatcagtat caccatcaat gtcacagaag gtgagcgttt cattgtcacc agcgtcaagc
     3721 tagcgggtga tttcctgggc aaggacgatg agttcaaaac tctggtggca attcgtcccg
     3781 ggcaagccta taacgccgat gaggtggcca agacgaccaa ggctttcact gat
//
LOCUS       DAOSLL010000484         3814 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_21077_length_3814_cov_5.378292, whole genome shotgun sequence.
ACCESSION   DAOSLL010000484 DAOSLL010000000
VERSION     DAOSLL010000484.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21077_length_3814_cov_5.378292"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="PLB25_17430"
     CDS             <1..463
                     /locus_tag="PLB25_17430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HOE43392.1"
                     /translation="AGRWLALPAGPMLLPLLLGAVLQALGLLVIELPAALLAVSYTVI
                     GWSIGLRFTRSILLHAWRALPQVLAAIFVLIGVGVLIATGLTLLAGLEPLTAYLATSP
                     GGADSVAIIAATSAVDAGFVMAMQLARFLMVLAFGPSVSKFTASRWQADQT"
     gene            complement(454..2550)
                     /locus_tag="PLB25_17435"
     CDS             complement(454..2550)
                     /locus_tag="PLB25_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HOE43393.1"
                     /translation="MSLPSHQLELLAPARDVAIGIEAVNHGADAVYLGGPSFGARASA
                     DNSVRDIARLVQHAHRFNSRIFVTLNTILRDDELEPARKLAWQLYDAGVDALIIQDLG
                     LLEIDLPPIQLHASTQTDIRSPEKARFLQDVGLSQLVLARELDLGQIAAIRAATDPER
                     CALEFFVHGALCVAYSGQCYISAANTGRSANRGECSQACRLPYQVVDAAGRFVAHDKH
                     VLSLKDNNQSANLAALVAAGVRSFKIEGRYKDMAYVKNITGHYRQLLDALMEKRQTAG
                     QPLARSSSGSTTLSFTPDPLQNFNREFTDYFVAGRKDDIGAFDTPKNPGLPIGHVTRL
                     GPNWVELELVNHQTLLHNGDGLCYYDLQKELVGMAINRAEAVNAAKGRWRVFPKDALD
                     SLKDLRCGLEVNRNRDIDWLRVLEKKSSDRRIGVWARLQVLAQSVQLTLTDEDGNFAS
                     VEQAFAADQRHLAQDANAAKAQACHQLSRLGDTIFIATHADVVWAAGQFDSIVLPSSL
                     LNGLRRAAVLALEQARLTAFVRLPPGVPVEPPARYPQDHLSYLANIFNSKAHAFYARH
                     GVRVIEPAFEALEELGEVSLMITKHCVRFALSLCPKQAKGITGVQGFVKAEPLLLVNG
                     KEKLTLRFDCKPCEMHVVGKMKTSVARQSRQQLAAASGGIPITFHKVRPTPVAGPDAA
                     QTGADDNLAPVGRAQV"
     gene            complement(2957..3601)
                     /gene="upp"
                     /locus_tag="PLB25_17440"
     CDS             complement(2957..3601)
                     /gene="upp"
                     /locus_tag="PLB25_17440"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019652493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HOE43394.1"
                     /translation="MTYNTDPLVHVINHPLVQHKLTLMRRKDASTNSFRTLLNELAML
                     MAYEVTRDMPTQDIEIETPLETMTARVIDGKKLVLVSILRAGTGILDGMLSVVPGARV
                     GHIGLYRDPATLQAVEYYFKMPKDMQERDVVVVDPMLATGNSAIAAIERIQVLNPKSV
                     KFVCLLTCPEGISALRRAHPQVPIYTAAVDRQLNDHGYILPGLGDAGDRIFGTQ"
     gene            complement(3687..>3814)
                     /locus_tag="PLB25_17445"
     CDS             complement(3687..>3814)
                     /locus_tag="PLB25_17445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HOE43395.1"
                     /translation="GGGRRTSAGEQLFKSAASSIGREVGRQLMRGVLGGLFGGKR"
BASE COUNT          663 a         1143 c         1239 g          769 t
ORIGIN      
        1 ggccggtcgc tggctggcgc tgccggccgg gccgatgctg ctgccgctgc tgctgggcgc
       61 cgtattgcag gcgctcgggc tgctggtgat tgagctgcca gcggcgctgc tggcggtttc
      121 ttataccgtg attggctgga gcatcgggct gcgctttacc cgcagcatcc tgttgcacgc
      181 ctggcgcgcg ctgccgcagg tgctggctgc catctttgtg ctgatcggtg ttggcgtgct
      241 gattgccacc ggcctgaccc tgctggccgg gcttgaaccg ctcaccgcct atctggccac
      301 cagccctggc ggcgctgatt cggtggccat cattgccgct accagcgcgg tcgatgccgg
      361 ttttgtcatg gcgatgcaac tggcgcggtt tctgatggtg ctggcatttg ggccgagtgt
      421 gtcgaaattc actgcgtcgc gctggcaagc tgatcagacc tgagcgcgac cgactggtgc
      481 caggttgtcg tccgcgccag tttgcgccgc atcaggacca gcgacaggtg ttggccgaac
      541 tttgtgaaag gtgatcggga tgccacccga cgctgcagcc aattgctgac gcgactgcct
      601 ggccaccgag gttttcatct tgcccaccac atgcatctcg cagggcttgc agtcaaagcg
      661 cagggtcagc ttttccttgc cattgacgag caacaagggc tctgccttga caaaaccctg
      721 caccccggtg atgcccttgg cctgcttcgg gcacaggctt agcgcaaagc gcacgcagtg
      781 cttggtgatc atcaggctga cttcgccaag ttcttccagc gcttcaaagg ctggctcgat
      841 cacccgcacc ccatgcctgg cataaaaggc atgggccttg ctgttgaaga tgttggccag
      901 gtaactcaaa tggtcttgcg ggtagcgcgc tggcggctcc accggcacgc ccggcggcag
      961 gcgcacaaag gcagtcaggc gtgcctgctc cagcgccagc accgccgccc ggcgcaggcc
     1021 attgagcagc gaactgggca aaactataga gtcaaattgg cctgcagccc ataccacatc
     1081 agcgtgagta gctataaaaa tagtatcacc caggcgactc aactggtggc aggcctgggc
     1141 tttggcggca ttcgcatcct gcgccaagtg gcgctggtcg gcggcaaaag cctgttccac
     1201 gctggcgaag ttgccgtctt cgtcggtcag cgtgagctgc acgctttgcg ccagcacctg
     1261 cagccgggcc cagacaccga tgcgccggtc gctggacttt ttctccagca cgcgcagcca
     1321 gtcaatgtcg cggttgcggt tgacttcgag cccgcagcgc aggtccttga ggctgtcaag
     1381 cgcatctttc gggaacaccc gccagcggcc tttggcggcg ttcaccgcct cggcccggtt
     1441 gatcgccatg ccgaccagct ctttttgcaa gtcgtagtaa cacagtccgt cgccgttgtg
     1501 cagcagggtc tggtggttta ccagttccag ctcgacccag ttcgggccaa gtcgggtgac
     1561 atggccaatc ggcaggccgg ggttttttgg cgtgtcaaaa gcgccgatgt cgtccttgcg
     1621 gccagcgaca aaatagtcgg tgaactcgcg gttgaagttt tgcagcgggt ccggcgtgaa
     1681 gctcagggtg gtgctgccgc tgctcgatct tgccagcggc tggccagcag tctggcgctt
     1741 ttccatcagg gcatcgagca gttggcggta gtggccggtg atgtttttca cataggccat
     1801 gtccttgtag cggccttcga tcttgaagct gcgcacaccg gccgccacca gggctgccag
     1861 gttggcactc tggttgttgt ccttgaggct tagcacgtgc ttgtcgtgcg cgacaaagcg
     1921 gccggctgca tccaccactt ggtacggcag gcgacaggcc tggctgcatt cgccccggtt
     1981 ggcgctgcgc ccggtgttgg cggcggagat gtaacactgg ccactgtagg ccacgcacag
     2041 cgcgccgtgg acaaaaaact ccagcgcgca gcgctcgggg tcggtggcgg cacggatggc
     2101 ggcaatctgg cctaggtcga gttcacgcgc cagcaccaac tggctaaggc ccacatcttg
     2161 caaaaagcgg gccttttctg ggctgcggat gtcggtctgg gtgctggcgt gaagctggat
     2221 cggtggcaga tcgatttcaa gcaggcccag gtcctggatg atcagcgcat caacccctgc
     2281 gtcatacagt tgccaggcca gtttgcgggc tggctcgagt tcgtcgtcgc gcaggatggt
     2341 gttgagggtg acgaagatgc ggctgttgaa gcggtgcgca tgctgcacca gccgggcaat
     2401 gtcgcgcacg ctgttgtcgg cgctggcgcg ggcaccaaac gacggtccgc caagatagac
     2461 cgcgtcagcg ccatggttga cggcctcgat gccaatggcg acatcgcggg ccggggctaa
     2521 cagttcgagt tgatgtgatg gcagggacat ggggccggat tatccatgcg ccacccattg
     2581 agccgggtcc ggcaattgcg caggggcggc atttccctgg tggcaggcgc agaccttgcc
     2641 cgcgcgccag tgaggtcccc ggcaggcctt ggcaaaacct ctggcgagaa tgctgcccat
     2701 cggatgatgg cccagagcag caacgcgaga gactggccga cttcaaaaag acaggtggca
     2761 aacaggtggc gcaatcatcc cgcgacggca ccttcagatg tcacaccccg gcgttgcaga
     2821 tcggcagcca gagcatcagt gcgtgtacat cacccatgcc aacaaggccg agctgaccat
     2881 caacggctga gcttgtggac ctggtcaaaa caggtcaaaa caggtcagga tgtgccggcg
     2941 actgcaccgc tggccatcac tgggtgccga agatgcggtc tccggcatcg ccaagtccgg
     3001 gcaggatgta gccgtggtcg ttgagctgtc ggtccacggc agcggtatag atcggcactt
     3061 gcgggtgcgc ccggcgcagt gcgctgatgc cctcggggca ggtcagcagg cagacaaact
     3121 tgaccgattt ggggttgagc acctgaatgc gctcaatcgc cgcaatggcc gagttgccgg
     3181 tggccagcat cgggtccacc accaccacgt cgcgttcctg catgtctttg ggcatcttga
     3241 agtaatactc cacggcctgc agggtggccg ggtcgcggta caggccaata tggccgactc
     3301 gtgcgccggg caccacgctg agcatgccgt ccagaatgcc ggtgccagcc cgcagaatcg
     3361 acaccagcac cagtttttta ccgtcaatca cccgggcggt catggtttcc agcggggttt
     3421 cgatttcgat gtcctgggtt ggcatgtcgc gcgtgacttc gtacgccatc agcatggcca
     3481 gttcgttgag cagggtgcga aagctgttgg tgctggcatc cttgcgacgc atcagcgtga
     3541 gcttgtgctg taccagcggg tggttgatga catgaacgag agggtcggtg ttgtaggtca
     3601 tgggttttgt tgaatattgg agatgtgccg aattcgcccg attgccgctg cgcagaaacc
     3661 gagggtgctg gtgatcgtgc gtttgatcaa cgtttgccgc caaacagccc gccgagcacg
     3721 ccgcgcatga gttggcgacc gacttcacgg ccaatcgagc tggccgcgct tttgaacaac
     3781 tgctcgccag ccgaggtgcg ccgaccccca ccac
//
LOCUS       DAOSLL010000485         3813 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_21088_length_3813_cov_3.380255, whole genome shotgun sequence.
ACCESSION   DAOSLL010000485 DAOSLL010000000
VERSION     DAOSLL010000485.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3813
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21088_length_3813_cov_3.380255"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(20..2089)
                     /locus_tag="PLB25_17450"
     CDS             complement(20..2089)
                     /locus_tag="PLB25_17450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HOE43396.1"
                     /translation="MAPDAVQAQIDRWICSMAGSQSTQSFHQRAALFISRQLTIVCLV
                     CAALCVTIWVITIKQIDQERIRAISRALESNTILAKTQERRLSNALQVLDQVLLVLRD
                     AYVAHGAPKSLNQRLEAMHVDRTYVGIITLIGETGDVIATTAENLNVNFSDREYFKDH
                     AADMTDRLLIGKPIIGRKTGKSLVSLTRRLYHPNGTFAGVVFLALDPVFLAPDYSNIE
                     LARDAAIALVGLDGIVRVRHTNGTNSFGDDVRSGQILHEVSKAANGNFIGVTPIDGQR
                     RAISYRKLPDHPLVVLVGYPTEQVTESLKGSERIYLLSATLGSLLVIALAAATNMALA
                     RSRKQLDEAAANAAKMRAIINASPVPMALNDSVGCVTFVNRAFVETFGYEPEDIPTLE
                     LWWACAYPDPLYRQWVIDTWGEELKRSEATGTRFAPLEIKLRCKDGSYKIAVASAANY
                     SQAIDAEHLVVLFDITEREAAEQALQSSLHEKEALLKEVHHRVKNNLQLITSLIRLEV
                     GRSTQAEAKSVLGEMQGRIRSMALLHESLYRNESFASIDLGRYVGQLASQVFSANLSR
                     NAPIELRLELTSVSASLDQAIPCGLLVNELVTNALKHGFPDGRSGSVSVELRPGDDNS
                     LWRLTVSDTGVGLPDDFESRRQSSLGLQLASGLANQLGGELNSGPGSKFAVSFSIAKP
                     APISMPG"
     gene            complement(2724..>3813)
                     /locus_tag="PLB25_17455"
     CDS             complement(2724..>3813)
                     /locus_tag="PLB25_17455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005793590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HOE43397.1"
                     /translation="QGGLVVDYIGIGNELKAAMKEYTQSKGRGRPTVDAAEAFSVVLE
                     KLDVLRAMLHGFDYSGFLTGGHKTLAGAANHVLGLRTAPSGVGSREGKKRFADAALAL
                     SQAFTLCCTLDEARQVREEVAFMQAVKVILTKKAISQQKKTDEQRELAIRQIISHAVV
                     SDSVVDIFDAVGLDKPDIGLLDDEFLAQVNSLPEKNLAVELLERLLEGEIKSRFASNV
                     VQNKKFSELLSNVIVRYQNRSIETAQVMEELVQMARKFREAAHRGEELGLTEDEVRFY
                     DALANNESAVRELTDETLKKIAHELTENLRQNLTVDWSERESVRAKLRLMVKRILRKY
                     KYPPDLQEAAVELVLQQAQVMGEAWAIA"
BASE COUNT          809 a         1089 c         1004 g          911 t
ORIGIN      
        1 atcagtggaa aacagccgtt caacccggca tcgaaattgg agcaggtttg gcgatgctga
       61 agctcacagc aaattttgac cctggacctg aattcaactc gccgccaagt tggttggcca
      121 agccggaggc gagttgcaga cccagcgaac tctgtcgcct tgactcgaaa tcatccggta
      181 gtccaacgcc agtatcgctt actgtcaaac gccagagcga gttgtcatcg ccggggcgca
      241 actcgactga aacgctacct gatcgcccat cgggaaagcc atgcttgagt gcatttgtga
      301 ccaactcatt gaccagcagt ccgcaaggaa tcgcctgatc aagactggcc gacaccgatg
      361 tcagttcgag tctgagttcg atgggtgcat ttctcgatag gttggcgctg aacacttgtg
      421 atgccaactg cccaacgtaa cgaccaaggt caatactggc aaatgattca ttgcgataca
      481 gcgattcatg gagcaaggcc atggatctga ttcgcccctg catttcgcca agaaccgatt
      541 tcgcttcagc ttgcgtgctt cgtccgacct ccagacggat caaactggtg atgagttgca
      601 ggttgttctt gacgcgatgg tggacctctt tgagaagagc ttctttttca tgcaaggacg
      661 attgaagagc ctgctcagcc gcttcgcgtt cggtgatgtc gaaaagaacg acaagatgct
      721 ctgcgtcaat ggcttgggaa taattcgcag cactggcaac ggcgattttg taactgccat
      781 ccttgcagcg caacttgatc tccaaaggag cgaaccgggt gccagtcgcc tctgatcgct
      841 tgagttcttc tccccaggtg tcgatgaccc actggcggta tagcggatct gggtaagcac
      901 aggcccacca gagctccaga gtcgggatgt cctctggctc atagccaaag gtttcaacaa
      961 aggcccggtt gacaaaggta acgcagccaa cagaatcatt caatgccatg ggaaccggtg
     1021 aggcattgat gattgcacgc atttttgctg cattggctgc ggcctcgtcg agctgcttgc
     1081 ggctgcgggc caacgccatg ttggttgctg cggccagagc gatgaccagc aagctgccca
     1141 aagttgcaga tagtaaatag attcgttcgc tgcctttcaa ggattcggtc acttgctcag
     1201 tgggatatcc caccaacacc accaaggggt gatctggcag cttcctgtag ctgattgcgc
     1261 ggcgttgccc atcaatgggt gtcacaccga tgaagtttcc atttgcagcc ttgctcacct
     1321 catgcaagat ttgtcctgat ctcacgtcat cgccaaagct gttggtgccg tttgtgtgac
     1381 gcactctgac aatgccatcc aagccgacta atgcaatggc ggcatctctt gccaattcga
     1441 tgtttgaata gtcaggggcg aggaagacag ggtcaagtgc cagaaagacc accccggcaa
     1501 aagtgccatt ggggtgataa aggcggcgcg tgagtgaaac cagcgacttt cccgtctttc
     1561 gtccaataat cggcttaccg atcagcagcc gatctgtcat atcggcggca tggtctttga
     1621 aatactctcg gtcggaaaaa ttgacattta ggttctcagc cgtcgtcgcg ataacatcac
     1681 ctgtttcacc gatcaaggta atgatgccga catacgtcct gtcaacatgc atcgcttcaa
     1741 ggcgttggtt cagtgacttg ggggcaccat gtgccacata ggcgtctcgc aaaaccagca
     1801 gaacctggtc caacacctgc aatgcgttgg aaagccgacg ttcctgggtc tttgcaagga
     1861 tggtgttgct ttccaacgca cgtgaaatcg cccgaatacg ttcctggtca atctgtttga
     1921 tggtgatgac ccagatcgtc acgcacagtg cggcacaaac gagacaaaca attgtcaatt
     1981 gccgcgagat gaacaatgca gcacgttgat gaaacgattg cgttgattgc gaaccggcca
     2041 ttgaacagat ccacctatct atttgtgcct gaactgcgtc aggtgccaag ctacttttga
     2101 cctactgctc aatccaattc gtagcagggc gcagctctaa ttgtccactg actactgggt
     2161 tgaagtgaat ttctgacggt ccaagtatgc cactgcccgg tcgctgtcaa aaaagccaca
     2221 gagctaacgg cactgtcgct ggcagcctcg cattgagttg ttgacgctca tcagcagtgc
     2281 ttgaattttg acttttgttg gcgactggtt cacctggccc acagcttttg acccagcact
     2341 acttttgcca tcattgcctc atgttccacc gactcacgcc gggtcatgga cgcaaggtat
     2401 tgacgatgac cagtggctcc agccgcactg tcagggcgat tccggactca ttggcggcgt
     2461 tggcagttga ttttcgggca gcgccggaca tcccccagcg cggggcgcgt cgcaccagaa
     2521 ggttgaacct tggtggccag caaagactcc tatttcacct ttatttgggt gcattagctt
     2581 ttgtccatcc cctggcgatc atgcagttgg ggtagtagtc gcacttcgct cgaatttggg
     2641 caccaagtgc ccacccggcg gcaaagccac ttgccgctgc actggtgcaa gttcgcacct
     2701 tggggcctat tgttcagatg cgctcaggca atcgcccagg cctcccccat cacctgcgcc
     2761 tgctgcaaca ccagttccac ggccgcttcc tgcagatcgg gcgggtattt gtatttacgc
     2821 agaatgcgct tgaccatcaa cctcagtttg gcccgcacgc tttcccgctc tgaccagtcc
     2881 accgtcaggt tctgacgcag gttctcggtc aactcatggg cgattttctt gagggtttca
     2941 tcggtcagct cgcgcaccgc tgattcattg ttggccaggg cgtcgtaaaa ccgcacctca
     3001 tcttcggtca agcccaattc ttctccacgg tgcgccgctt cccggaattt cctagccatc
     3061 tggaccagtt cctccatcac ctgcgcggtt tcgatggagc gattctggta acggacgatc
     3121 acgttgctca gcagttctga gaatttcttg ttctgcacca cgttgctggc aaaccggctc
     3181 ttgatttcgc cttcaagcag ccgctccagc aattccactg ccaggttctt ctcgggcagg
     3241 ctgttgacct gtgccagaaa ctcatcatcg agcaggccga tgtcgggttt gtccagaccc
     3301 acggcatcaa agatgtccac cacgctgtcg gacaccacgg cgtggctgat gatctggcgt
     3361 atcgccagtt cacgctgttc atcggtcttc ttttgctggc tgatggcctt tttggtcagg
     3421 atgaccttga ccgcctgcat gaaagccacc tcttcgcgca cctgtctggc ctcatcgagg
     3481 gtacagcaca gggtgaaggc ctggctcaag gccagcgctg catcggcaaa gcgctttttg
     3541 ccttcgcggc tgccgacacc cgatggtgcg gtcctgagcc ccaacacatg gttggcggca
     3601 ccggccaggg ttttgtgacc accggtgaga aatccgctgt agtcaaagcc gtgcagcatg
     3661 gcccgaagga catcgagctt ttccagcacc acactgaacg cctccgccgc gtccacagtc
     3721 gggcggcccc ggccttttga ctgggtgtac tctttcatcg cggctttgag ttcgttgccg
     3781 atgccgatgt aatccaccac cagcccacct tgt
//
LOCUS       DAOSLL010000486         3803 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_21144_length_3803_cov_4.130736, whole genome shotgun sequence.
ACCESSION   DAOSLL010000486 DAOSLL010000000
VERSION     DAOSLL010000486.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21144_length_3803_cov_4.130736"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1461
                     /locus_tag="PLB25_17460"
     CDS             136..1461
                     /locus_tag="PLB25_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43398.1"
                     /translation="MTTAPFPETILHPSSAEALNRDCFCRTLNRERLREQLESDPSLK
                     GLMDNIALTRPNLFSSSVVFVSDKVQQQIMATVAAIERVAALPGYLAQALSRADAIAS
                     FDFGPRAVCMGYDFHLSAEGPKLIEINTNAGGLLLNAALARAQEACCDAFDWVFTSFA
                     RHDALRQSFFDMFASEWQLQRGSAPWRNVLIVDRAPQQQYLAPEFELFRQLFAQHGIS
                     AQIADPGELLWQDGHLTYQNQQVDLVYNRLTDFYLDQPDHLALRLAYQAGAVVLTPHP
                     RAHALLADKRNLIAFSQDSLLTAWGVNAADRKLLASSVPSTRLVTPERADELWAQRRQ
                     LFFKPVAGYGAKAAYRGDKLTRRVWGDILAGDFVAQALVPPGERMIELDGVLTDLKFD
                     IRAFTYAGQVQMLAARMYTGQTTNFRTQGGGFAPVIVVPASNQLGAGAR"
     gene            1461..3269
                     /locus_tag="PLB25_17465"
     CDS             1461..3269
                     /locus_tag="PLB25_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglutaminylglutamine amidotransferase"
                     /protein_id="HOE43399.1"
                     /translation="MCGICGELRFDGNVPDMAAIARMSAVLARRGPDDAGSYQNGPVG
                     LGHRRLSIIDLSAQAHQPMVDATLQLTLVFNGTIYNYRELRVELLGLGYRFFSEGDSE
                     VILKAYHAWGQTCVTRFKGMFAFAIWDQRSGQLWLARDRFGIKPLYLAQDAKRLRFAS
                     SLPALLAGGGVDTQLDPVALHHHFTLHTVVPAPRTVLRGVRKLPPACSMMLQVDGSVT
                     SQVYWTLDATRPAHDLSEAQWLAATRSALDQSVERRLLAADVPVGVLLSGGLDSSLLV
                     GLLADHVPDLRTFSIGFEDLGGGAEKADEFEYSDQIADLFKTRHHKFCIPNSEVLTRL
                     PEAVAQMTEPMVSHDVIAFFLLGEKVSKEVKVVMSGQGADEVFGGYFWYPRMDAEAGT
                     PLARFSQHYFDRDHAEYLQMIMPAFQVGDVTSALVATELTKPLADEFLDQVWRFDATT
                     LIVDDPVKRVDNMPMAWGLEVRTPFLDHELVELAAKMPPALKLKEGGKFPLKAISRGL
                     IPDSVIDRPKGYFPVPALRYVRGPFLQLMRDILMSDACMQRGLYQRDYVDRLLAAPEA
                     PEHFTRINGSKLWHLALLEWWLQVHVDGCQMPPEAL"
     gene            complement(3291..>3803)
                     /locus_tag="PLB25_17470"
     CDS             complement(3291..>3803)
                     /locus_tag="PLB25_17470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43400.1"
                     /translation="VLGTLGRGLSALAVFVAMAVGWTLLVFFFSPWFSAESNRWPSAC
                     PIIVGSAETGQTRVILYHRFAAEQKADPTLVPWPLVPAGTGIEGQVRTVWQSVTGKAW
                     RYEARWDDGDYILESRYRLDGTRPVLVESRRRDPSLGLIGILLALFTLVIWKAVQWWQ
                     QRRCIQAGPQ"
BASE COUNT          699 a         1194 c         1146 g          764 t
ORIGIN      
        1 ccatattttt cgacgatccg ggtgtttttt cagcttcagg caaaatcagg gcatgatcac
       61 gtcggcagcc aggttttccg gctgttgcag gcaaacactg atgcctggcc gtgacccact
      121 gccagaaatt ctttcatgac cactgcccct tttcccgaga ccatcttgca cccgagttct
      181 gccgaggcgc tgaaccgcga ttgtttttgt cgcaccctga accgggagcg gctgcgcgaa
      241 caattggaga gcgaccccag cctcaagggc ctgatggaca acatcgccct gacgcggccc
      301 aacctgtttt caagcagtgt ggtgtttgtc tctgacaagg tgcagcagca aatcatggcc
      361 acggtcgcag cgattgagcg cgtcgccgcc ctgcccggtt acctggcgca ggccctgtca
      421 cgggcggatg cgatcgccag ctttgacttc ggccccaggg cggtctgcat gggttacgac
      481 ttccacctca gtgccgaggg gccgaaactg atcgaaatca acaccaacgc cggtggcctg
      541 ttgctcaacg ctgctttggc gcgcgcccag gaagcctgtt gcgatgcgtt cgactgggtg
      601 ttcacgtcgt ttgcccggca cgacgcgctc aggcagagtt tctttgacat gtttgccagc
      661 gaatggcaac tgcaacgcgg ctcggccccg tggcgcaatg tgctgattgt cgacagggcc
      721 ccacagcagc aatacctggc acctgagttc gagctgtttc gccagttgtt tgcgcagcat
      781 ggcatcagcg cccagattgc cgaccccggc gagctgctct ggcaagacgg ccacctgacc
      841 taccagaacc agcaagttga cctggtctat aaccgcctga ccgactttta cctcgaccaa
      901 cccgaccacc tggccctgcg actggcttat caggccggtg ccgtggtgct gaccccgcac
      961 ccgcgcgccc atgcgctgct ggccgacaag cgcaacctga ttgcgttcag ccaggacagt
     1021 ttgttgacgg cgtggggggt caatgccgcc gaccgcaagc tgctggccag cagcgtaccc
     1081 agcacccgac tggtgacacc ggaacgagcc gacgaactgt gggcacagcg ccgccagttg
     1141 ttctttaagc cggtggccgg ttacggggcc aaagccgctt accgcggcga caagctgacc
     1201 cgccgggtct ggggcgacat tctggccggt gactttgtcg cgcaggcact ggtgccgccg
     1261 ggtgagcgca tgattgagct cgacggcgtg ttgaccgacc tcaagtttga tatccgcgcc
     1321 ttcacctacg cggggcaggt acagatgctc gctgcgcgca tgtacaccgg acagaccacc
     1381 aacttccgca cccagggtgg cggtttcgca ccggtgattg tggtgcccgc cagcaaccag
     1441 cttggtgcgg gagcccgctg atgtgcggca tttgtggtga acttcgtttt gacggcaatg
     1501 tgcccgacat ggcagccatt gcccgcatgt ccgctgtgct ggcacgccgt ggccccgacg
     1561 atgcgggcag ttaccagaac ggtccggttg gcctgggcca ccggcgcctg tcaatcattg
     1621 acctgtcggc ccaggcgcac cagccgatgg tcgatgccac gctgcaactc acgctggtgt
     1681 tcaacggcac catttacaac tatcgcgagt tgcgggtcga gctgctgggc ttgggctatc
     1741 ggtttttttc cgaaggcgac agtgaagtca tcctgaaggc gtaccacgcc tgggggcaga
     1801 cctgcgtgac acggttcaag ggcatgtttg cctttgccat ctgggaccag cgcagtggcc
     1861 agttgtggct ggcgcgcgac cgttttggca tcaagccact gtacctggcg caagacgcca
     1921 agcgcctgcg ctttgcctca agcctgccgg ccttgctggc cggtggtggt gttgatacgc
     1981 agcttgaccc ggtggcgctg caccaccact tcacgctgca cacggtggtg ccagcgccgc
     2041 gcaccgtgct gcgcggcgtg cgcaagctgc cgcccgcttg cagcatgatg ctccaggtcg
     2101 atggcagcgt caccagccag gtctactgga cgctcgatgc cacccggccc gcgcatgatt
     2161 tgtctgaggc ccagtggctc gccgccaccc gatcagcgct ggaccagagt gtcgagcgtc
     2221 gtctgctggc agccgacgtg ccggtcggcg tgctgctgtc aggcgggctc gactccagcc
     2281 tgctggtcgg cttgctggca gaccatgtgc cagacctgcg caccttttca attggttttg
     2341 aagacctcgg cggtggtgcg gaaaaagccg atgagtttga atactcggac cagatcgccg
     2401 acctgttcaa aacccgccac cacaaattct gcattcccaa cagcgaggtg ctgacccggc
     2461 tgcccgaagc cgtagcgcaa atgaccgagc cgatggtcag ccatgacgtg attgcatttt
     2521 ttctgctggg cgaaaaggtc tccaaagagg tcaaggtggt gatgtccggc caaggcgctg
     2581 atgaggtgtt tggcggctat ttctggtacc ccaggatgga tgccgaagct ggcacgccgc
     2641 ttgcgcgatt cagccagcat tactttgacc gcgaccatgc cgagtatttg cagatgatca
     2701 tgccagcgtt tcaggttggc gatgtcacgt ctgcgctggt tgccaccgag ttgaccaagc
     2761 cactggccga tgagtttctg gaccaggtct ggcgctttga tgccaccaca ctgattgttg
     2821 atgacccggt caagcgggtg gacaacatgc cgatggcctg gggccttgag gtgcgcacac
     2881 cgtttcttga ccacgaactg gtggaactcg ccgccaaaat gccaccggcg ctcaaactca
     2941 aggagggtgg caagtttccg ctcaaggcga tttcgcgtgg cctgattcct gattcggtaa
     3001 tcgaccggcc caaaggttac tttccggtgc ccgcgctgcg ctatgtgcgc gggccatttc
     3061 tgcaactgat gcgcgacatc ctgatgtctg atgcctgcat gcagcgcggg ctttaccagc
     3121 gcgactatgt tgacaggctg ctggctgcac cggaagcacc agagcatttc acccgcatca
     3181 acggcagcaa gctgtggcat ctggcactgc tggaatggtg gctgcaagtg catgtggatg
     3241 gttgccaaat gcccccggag gcgctctgaa cacctttgac cagcgtcggc ctactgcggt
     3301 ccggcctgaa tacaccgccg ctgctgccac cattgcactg ctttccagat caccagggtg
     3361 aacagcgcga gcaatatgcc gatcaatccc agacttgggt cgcgtctacg gctttcaacc
     3421 agcaccggac gggtgccatc gaggcggtag cggctctcca ggatgtagtc gccatcatcc
     3481 cagcgcgcct cgtagcgcca ggccttgcca gtaaccgatt gccacaccgt tcggacctgc
     3541 ccctctattc cggtgccagc aggcaccaac ggccacggca ccagagttgg atctgccttt
     3601 tgttctgcgg caaaccgatg gtacagaatc acccgggtct gaccggtttc ggcgctgccc
     3661 acaatgatcg ggcatgcact tggccagcgg tttgactcgg cactgaacca ggggctgaag
     3721 aaaaacacca gaagggtcca gccgaccgcc atggcaacaa aaacggccag tgccgacaag
     3781 ccgcgcccta gcgtaccaag gac
//
LOCUS       DAOSLL010000487         3794 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_21192_length_3794_cov_4.154587, whole genome shotgun sequence.
ACCESSION   DAOSLL010000487 DAOSLL010000000
VERSION     DAOSLL010000487.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21192_length_3794_cov_4.154587"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(342..1172)
                     /gene="cysQ"
                     /locus_tag="PLB25_17475"
     CDS             complement(342..1172)
                     /gene="cysQ"
                     /locus_tag="PLB25_17475"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008247704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HOE43401.1"
                     /translation="MLVTKEQLATLCETAEMAGREVMAVYAEDFSASFKADASPLTEA
                     DLRADRVIRHELEAKFPGIFILSEESVSLGLGPSDTFFLVDPLDGTKEFLKRNDEFTV
                     NIALVHKAKVMAGVVLAPALGELFFAAPGLGVWKRDGQGTHAMKVVNFDEDRPLRVIG
                     SRSHGGGPLSNWLSQLPCQHDFLAAGSSLKFCRIAEGAADIYPRFGPTSQWDTAAAQC
                     VLEVAGGAVTDLVGQTLTYGMDRAVLNPSFVAAGDSDLLGWVDKSDDASSPASVTPLM
                     "
     gene            complement(1768..1844)
                     /locus_tag="PLB25_17480"
     tRNA            complement(1768..1844)
                     /locus_tag="PLB25_17480"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1808..1810),aa:Arg,seq:tct)
     gene            2225..3124
                     /gene="cysD"
                     /locus_tag="PLB25_17485"
     CDS             2225..3124
                     /gene="cysD"
                     /locus_tag="PLB25_17485"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014429326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HOE43402.1"
                     /translation="MSLTHLQRLEAESIHIMREVVAEADKPVMLYSIGKDSAVMLHLA
                     MKAFYPAKPPFPLLHVDTRWKFREMYAFRDRMVRELGLELLVYTNPEGIKKGINPFTH
                     GSAIHTDIMKTEALKQALDLYGFDAAFGGARRDEEKSRAKERIFSFRTAQHRWDPKNQ
                     RPELWRIYNVRKNVGESIRVFPISNWTELDVWQYIYLQKIPIVPLYYAAERPVVARDG
                     CLIMVDDERLPLLPGEVPMMKKVRFRTLGCYPLTGAIESQADTLPEIIQEMLLTTTSE
                     RQGRVIDHDSAGSMEKKKQEGYF"
     gene            3124..>3794
                     /locus_tag="PLB25_17490"
     CDS             3124..>3794
                     /locus_tag="PLB25_17490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012838119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOE43403.1"
                     /translation="MSHVSDLIATDIGQYLQSHEHKSLLRFITCGSVDDGKSTLIGRL
                     LYESKMLLEDQLAAVVADSRKFGTQGDQIDFALLVDGLTAEREQGITIDVAYRFFSTD
                     RRKFIVADTPGHEQYTRNMVTGASTADAAILMVDARKGVLTQTRRHSYLVWLLGIRHF
                     AVSINKMDLVNYSESTYRRIVDDYQNFARQLGIEDVTFIPMSACKGANIVSPSPNMPW
                     FHGTT"
BASE COUNT          867 a         1149 c          994 g          784 t
ORIGIN      
        1 tcgttgattt cagaatggca agcaggctat ttgacttgcc cggcacactc gattggcact
       61 gtgttttttt taatccggtg agctttaggg ccggatttag ctgaagcgcc aagcacgcga
      121 aagcgacggt gctggtttgc catttcggca gatgcgtcgc aggtgcggtc tgcagcagcg
      181 catgccgggt tgcggctggc aggcgccaag cctgccaatc tattgtgcaa aaaaaggcct
      241 ctagccctta tcagatatgc gctgacagct tcactttcga tagcatcatg aagcgaactt
      301 tgttttcaca gatggcgaac gggtgatcgc ccgcgagccc ctcacatcag cggcgttacc
      361 gatgccggcg aactggcatc gtcagactta tccacccagc ccagcaagtc gctgtctcct
      421 gctgcgacaa aacttgggtt taacaccgcg cgatccatgc cgtaggtcag cgtttgtccg
      481 acaaggtcgg ttaccgcgcc accagcaact tcgagcacac actgggccgc ggcggtgtcc
      541 cactgactgg ttgggccaaa gcgcggatag atgtccgccg caccttcggc aatgcgacaa
      601 aacttcaggg agctaccagc agccaggaag tcatgctggc agggcaactg tgacaaccag
      661 ttcgaaagcg gcccgccgcc atgagagcgg ctgccaatca cgcgcagcgg tcgatcttca
      721 tcgaagttga caaccttcat ggcatgggtt ccctgcccat cgcgcttcca gacccccagg
      781 ccaggtgcag caaagaacaa ctcacccaga gcaggggcca acacaacacc agccatcact
      841 tttgccttgt gtaccagcgc gatgttgacc gtgaactcgt cattgcgctt gaggaattcc
      901 ttggttccgt ccagcggatc aaccaaaaaa aacgtgtccg atggaccaag acccagggac
      961 actgattctt cggacaagat gaaaatgccc ggaaatttcg cttccagctc gtgcctgatg
     1021 acgcggtcag cccgcaagtc ggcctcggtc agcggcgacg catctgcttt gaaactggca
     1081 gaaaaatcct ctgcatacac cgccatgacc tctcgcccgg ccatttcagc ggtctcgcac
     1141 agagttgcga gttgttcttt tgtgactaac atccaacgac actccaccac agttcaattt
     1201 ttatcaacaa tcaaaccact gtcagtatgc cgcccaacag cagacaacgc aagcatacgc
     1261 cagactttcg catcgcagac ttgcgtcgcg gctgcattcg ttcaccaccg cacctggcac
     1321 aaggatcagc agacccgttg ggcggtcaag ttttggccac ccggcatgac tgctggacac
     1381 ctgggcggac cccaaccggg gtcgtttcga taagcccgga tagaaccgga cctgatctcc
     1441 aaggctacca gcctggaccg gcgggctctc acgcaaaaca ccagccgctg cgccctggaa
     1501 gaatttgatg aactcgggcg catcccaaag ctgacccagc acacgctggt gcatggggct
     1561 tgacacctta atcaatttct aaatcatcca tgccgttgct atctcgcctt gccttgcgtt
     1621 tgaaatgctt gcgccagggt accccgacag gaatgatttt gaaatggaat gacagatggt
     1681 gcctgaccgc acgcaagact tatgccatcg atggcaataa tcgacaccag cggggccagt
     1741 ttgcaccggc acttgaacag ttgtcagtgg ccagcccgga gggactcgaa ccccctaccc
     1801 atgtcttaga aggacattgc tctatccgga tgagctacgg gctgacagtt gaccatttta
     1861 tgcgacgcgt tcagtcacaa aattttgccc tggcgaaccc tggcgtgaca cgctggtttg
     1921 acgcaacggc tactatactg atcctggtat ctgatgacca aaacctgact gatttttctt
     1981 cacatctgac accacaccat gccctgtcgg ctcaattgaa gtatcccgag gcacagacct
     2041 gttcagcgcc tggggcttgt tccaaaaaac tgactccttt gtgatctgta gaagtcgccg
     2101 tcgcagccgg gcaactgcaa tcaatcgatc aggcccggtt gagccagatg aaaaaccagt
     2161 tgctgcaact caatcacgcc attcgaccca ctcttttttc gacccggtgg ctatcgggaa
     2221 cttcatgtca ctcacacact tgcaacggct tgaggccgag agcattcaca tcatgcgcga
     2281 agtcgtggcc gaagccgaca aacccgtcat gctttactca atcggcaaag acagcgctgt
     2341 catgctgcac ctggccatga aggcctttta tccggccaag ccgccttttc cgctgctgca
     2401 tgttgacacg cgctggaagt ttcgcgagat gtacgcgttt cgtgaccgca tggtgcgtga
     2461 gctagggctc gaactgctgg tttacaccaa tcctgaaggc atcaaaaaag gcatcaaccc
     2521 gttcacccat ggttcggcca tacacactga catcatgaaa acagaagcgc tcaagcaggc
     2581 cctggacctg tacggtttcg atgcggcctt tggcggtgcc aggcgcgacg aagaaaagtc
     2641 ccgcgccaaa gagcgcatct tttccttccg cacggcacag caccgctggg accccaaaaa
     2701 tcagcgcccg gaactctggc gcatttacaa cgtccgcaag aatgtcggcg agtcgattcg
     2761 cgttttcccg atctccaact ggaccgagct tgatgtctgg cagtacatct acctgcagaa
     2821 aattccgatc gtgccgttgt attacgcggc cgagcggccg gtcgtcgcgc gcgatggctg
     2881 cctgatcatg gtcgatgatg aacgcctgcc gctgctgcct ggagaagttc cgatgatgaa
     2941 aaaggtgcgc ttcaggacgc tgggctgcta cccgctgact ggtgcaattg aatcgcaggc
     3001 cgacacgctg ccagagatca tccaggaaat gctgctcacc accacctccg agcgccaggg
     3061 ccgcgtgatc gaccatgaca gtgctggctc gatggaaaaa aagaaacagg aaggctactt
     3121 ctgatgtcac acgtgtctga cctgatcgcc accgacattg ggcaatacct gcagtctcac
     3181 gagcacaaga gcctgctgcg attcatcacc tgtggcagcg tcgatgatgg caaaagcacc
     3241 ctgatcggac gcttgctgta tgaatccaaa atgctgcttg aagaccagtt ggcagcggtc
     3301 gtggccgact ccaggaaatt tggcacgcaa ggcgaccaga ttgactttgc cttgctggtg
     3361 gacggcctga ccgccgagcg cgaacagggc attaccatcg atgtcgccta ccggtttttc
     3421 tccaccgacc gccgcaaatt catcgtcgcc gacacgcccg gccacgagca gtacacccgc
     3481 aacatggtga ccggtgcgtc aaccgcagat gccgccattc tgatggtgga cgcgcgcaag
     3541 ggcgttctga cgcaaacccg gcggcacagt tacctggtct ggctactggg catccggcac
     3601 tttgcggtgt cgatcaacaa gatggacctg gtcaactaca gcgaatcgac ctatcggcgc
     3661 atcgttgatg attaccagaa ctttgcccgc cagctcggca tcgaggatgt caccttcatc
     3721 ccgatgtcgg cctgcaaggg cgccaacatc gtcagcccaa gcccgaacat gccgtggttc
     3781 cacggcacca cttt
//
LOCUS       DAOSLL010000488         3790 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_21218_length_3790_cov_4.610442, whole genome shotgun sequence.
ACCESSION   DAOSLL010000488 DAOSLL010000000
VERSION     DAOSLL010000488.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21218_length_3790_cov_4.610442"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="PLB25_17495"
     CDS             complement(<1..415)
                     /locus_tag="PLB25_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43404.1"
                     /translation="MKDGKTRHTFLKKLACGATLVSALGVAAPAHALYINLTSTGNAT
                     ADTGFRAAANIWQGVFTDNVTVNITAGFAALGTNILAQAGSTYFSTDFSTMKSALTAD
                     STSAEDAIMVAGLPGGSSYWTVPSSPRYSGGFLARR"
     gene            complement(984..1139)
                     /locus_tag="PLB25_17500"
     CDS             complement(984..1139)
                     /locus_tag="PLB25_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43405.1"
                     /translation="MTTRLAAEHAAASGELTTSSPAAHAVAVNATLVSRDRALAPAPE
                     LLWLEAI"
     gene            1194..1433
                     /locus_tag="PLB25_17505"
     CDS             1194..1433
                     /locus_tag="PLB25_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOE43406.1"
                     /translation="MSRLTIPLSEPCYRALKEAAAQRDKTIGQVSDESLDFCGIKSRE
                     QALDLVRRARAPSTLSEPQALALAKEAVAAERHAK"
     gene            1430..1861
                     /locus_tag="PLB25_17510"
     CDS             1430..1861
                     /locus_tag="PLB25_17510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
                     /protein_id="HOE43407.1"
                     /translation="MSGFPTIDTNVVVVGLLTGHAYSPVARILDGMLSAAFPFVVSEA
                     LLAEYRAVLLRSKLCKRHGLSVAEIDTFMTDLARHAIVLNPSNTVTAPITPDAGDQIL
                     WNLSASRADLVLVTGDKLLLQDSTMLHRIILPQTFMAQWQH"
     gene            1963..2730
                     /locus_tag="PLB25_17515"
     CDS             1963..2730
                     /locus_tag="PLB25_17515"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HOE43408.1"
                     /translation="MPRFHCPIPLTAGALLELPPGAARHVQVLRLQPGDAITLFAGEA
                     MAADTSESAISGEFAATVTRMGRSSVQVQVGAFTAWQREPSQRVHLAVGMPANERMDW
                     LVEKASELGVASIQPLMSARSVLKLSGERAEKKRLHWQSVAIAACEQCGGNRVPLVHA
                     VLPLDHWLKAQPASASGPQQRLLLSLSATAMGLREVSAGFSATADITFLSGPEGGLSP
                     AEEAAALQGKFAPVTLGPRVLRAETAALAALAALLVR"
     gene            2900..3331
                     /locus_tag="PLB25_17520"
     CDS             2900..3331
                     /locus_tag="PLB25_17520"
                     /inference="COORDINATES: protein motif:HMM:NF016807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HOE43409.1"
                     /translation="MLIRVIYTSTAVVPQTSAVTESILRTAQAANSQNGITGVLCQGQ
                     GVYLQALEGDRRTVTDLYQRIFADKRHTNLEMIHCECIAKRRYANWSMAHVAMSDLDP
                     ATKIDWPEFDPYSAAGMLVMGRIDELLAAGTVIKLADSEPH"
     gene            complement(3517..3714)
                     /locus_tag="PLB25_17525"
     CDS             complement(3517..3714)
                     /locus_tag="PLB25_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43410.1"
                     /translation="MGYKRFIGQPENICNDEYRLNVEKSCARLTVCVADLVPARPAKF
                     EICRPITADPAVLRGRPDNDG"
BASE COUNT          804 a         1085 c         1107 g          794 t
ORIGIN      
        1 tgcgcctggc caagaaaccg cctgaataac ggggagaact tggcactgtc cagtaacttg
       61 atccaccggg caatccggcg accatgatgg catcttcggc actggtgcta tccgcagtga
      121 gcgctgattt catggtcgaa aaatccgttg agaaataggt tgagccagcc tgggccaaaa
      181 tattggtacc aagcgcagca aatcctgccg tgatattgac ggtgacgttg tcagtaaata
      241 cgccctgcca gatattggcg gccgcccgaa agccagtatc cgcggttgca ttgcctgtcg
      301 aggtcaggtt gatgtacagc gcgtgggctg gagctgctac acccaaggcg ctgaccaagg
      361 ttgcaccgca ggccaatttt tttagaaacg tgtgcctggt ttttccgtct ttcatttggt
      421 ctccaatttc aaatgcaggt tgatgatttc atcaagccag gtgaggacgc ctggctttca
      481 ccgaacgaaa ttcatcgatt gttggcatca ctatttgtca gagccaaagg tcgtacagat
      541 caatgaaatc aggtaaaagc acgatccgcg ccatgtgctg taaatccaac aatttcaatg
      601 acatacatca aaaacaaaac ccaaaagcaa ggaggttgta aaaaatatcg acatttttca
      661 tgaaaactgg gtttgctgtg gggcggcggg cgacgactct tcttgcccca tctgtccctc
      721 aagcccctgc caagttaaac gtcggtttgc tgcattgaac atggtcaagg cactctttgt
      781 tgccgggccg cacctgaaca tctacaacct gctgacgctg tagtggatgg ccatgctggc
      841 ggtcaagctc aaagggtaat tggggtcagg taaatcggat cagagtcgaa taattctccg
      901 cagcgcaagt ccggcaagta ccgcattttt tataccaaat cagcctgtag cccttgtcca
      961 gaaagcgctg atagctccca tttttatatc gcctccagcc acaacagctc aggcgccggt
     1021 gccaaagcgc ggtcacggct gaccaaggtg gcgttcaccg caaccgcatg ggctgcggga
     1081 gacgatgtcg tcaattcgcc gcttgccgct gcatgttctg cggcaagtcg cgtcgtcatg
     1141 tgaggggtgc gaggtagctt attggtgtct taacaccaac cagggtcatg tccatgagcc
     1201 gtctgacaat ccccttgtct gaaccctgtt accgggcgtt gaaagaggct gctgcccaac
     1261 gcgacaaaac cattgggcag gtgagtgacg aaagccttga cttttgtggc atcaagtcgc
     1321 gcgaacaggc ccttgacctg gtgcgccggg ccagggcacc cagcacactg agcgaaccac
     1381 aagccctggc actggcaaaa gaagcggtgg ctgccgagcg ccacgcaaag tgagcggctt
     1441 cccaaccatt gacaccaacg tggtggtggt tgggctgctg accgggcatg catattcgcc
     1501 ggtggcacgc attttggacg gcatgctgtc tgctgcattt ccctttgtgg tgtctgaagc
     1561 cttattggcc gagtaccgcg ctgtgctgct acgttccaag ctgtgcaagc ggcatggctt
     1621 gtcagtggcc gagatcgaca ccttcatgac ggatctggcc cgccacgcca tcgtgctgaa
     1681 cccgtcaaac acggttaccg cgccgatcac cccagatgcc ggtgatcaaa tcttgtggaa
     1741 tttgtcggct tcccgagccg acttggtgct ggtgaccggc gacaagctgc tgctgcaaga
     1801 cagcacgatg ctgcatcgca tcatcctgcc gcaaaccttc atggcgcagt ggcagcactg
     1861 aaccaaaaga ggccaggctc gaacgacgca ttccgggtcg gcatggaggt aagcttcatc
     1921 atttgtcgca acaccattca acagccgctg ctcgccatcg ccatgccccg ttttcactgc
     1981 cccatccccc tcactgccgg tgcgctgctg gaattgccgc caggcgcggc gcgtcatgtg
     2041 caggtgttgc ggctgcagcc aggcgatgcc atcacgctgt ttgccggtga agccatggca
     2101 gccgatactt ccgagtcggc aatcagcggc gaatttgccg ccaccgttac caggatgggc
     2161 cgcagcagcg tgcaggtgca agtgggggcg tttaccgcct ggcagcgcga accctcacaa
     2221 cgggtgcatc tggcggtcgg catgcctgcc aatgaacgca tggactggct ggtcgaaaag
     2281 gcaagcgagc tgggcgtggc cagcatccag ccgctgatgt ccgcgcgtag cgtgttgaag
     2341 ctctcgggcg agcgggccga aaaaaagcgc ctgcattggc aaagtgttgc catcgccgcg
     2401 tgcgagcagt gcggtggcaa ccgggtgcca ctggtacacg cggtgctgcc gctcgatcat
     2461 tggctgaaag cccagcctgc ctcagccagc gggccgcagc aacggttgtt gctgtcattg
     2521 agcgccactg caatgggttt gcgtgaggtg tcggcgggat tttcagccac tgcagacatc
     2581 acatttttgt ccggtccgga aggcgggctg agcccggctg aggaagccgc cgcactgcag
     2641 ggcaagtttg cgccggtcac gctggggcca cgcgtgctgc gggccgaaac tgcggcgctg
     2701 gcagccctgg cggcgctgct ggtgcgctga acagccacag gcgtggccgt ccctggcatg
     2761 ggtcatgtgc tgcgctgctg tctgcaagac gcagcggggt gaggtcttcg gcgcgatgct
     2821 tggcactggc atgaacaatg ctacagaatc gcgccttcaa tctgtgacaa caacccgaaa
     2881 cccatgagga tatctgacca tgcttattcg tgtgatttac accagtaccg ctgttgtccc
     2941 gcaaacaagc gctgtcaccg agtcgatctt gcgaaccgcg caggcagcca attcgcaaaa
     3001 cggcatcacc ggcgttttgt gccaggggca gggtgtctac ctgcaggcgc tcgaaggtga
     3061 ccggcgcacc gtcactgacc tgtaccagcg catttttgcc gacaagcgcc acaccaatct
     3121 ggagatgatc cactgcgagt gcattgccaa aagacgctat gccaactggt ccatggcgca
     3181 tgtcgccatg tccgacctgg acccggcgac gaaaatcgac tggcctgaat ttgacccgta
     3241 ctcggcagcg ggcatgctgg tgatgggacg cattgacgag ttgctggccg ccggaaccgt
     3301 catcaagctg gccgacagcg agccacatta gcaatttgcc acgacgcagc gcgcccagac
     3361 ccgatgagcc aaatggggaa gtgatggatg accatgaccc aattggcccg gcttgtcatc
     3421 tggtgcgctg ttcgccgcca agcggcagaa ggtgccaagg caaccgcttt cggcttgcac
     3481 gcctgattct tgtttgctga aattcggggc agtcgttcag ccgtcgttgt ccgggcgacc
     3541 gcgcagcact gccgggtcag cggtgatcgg gcggcagatt tcaaacttag ccggacgagc
     3601 cggaaccaaa tcggcaacac aaaccgtcag tcgtgcgcaa gatttttcga cgttcagccg
     3661 atattcgtca ttgcaaatgt tttcgggttg accaatgaaa cgtttgtagc ccattgggtt
     3721 taggcgctat gcgcatcaaa cttaccaaca tgttgtcgat ctttgccgac agtctcgggc
     3781 agcaggcatc
//
LOCUS       DAOSLL010000489         3779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_21306_length_3779_cov_4.835661, whole genome shotgun sequence.
ACCESSION   DAOSLL010000489 DAOSLL010000000
VERSION     DAOSLL010000489.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21306_length_3779_cov_4.835661"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            334..972
                     /locus_tag="PLB25_17530"
     CDS             334..972
                     /locus_tag="PLB25_17530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43411.1"
                     /translation="MYFRILNDDASGELTYLLADLSGREAVLIDPHGRDLPVLSALLS
                     DRDLRLRWVLRTHHHDDRQTREPELLARLEAPMVQGDASRDTPVVGDGTVLPFGDELI
                     HVVATPGHTPHCLSFAWRDRFFCGALLAVTACPHQARPQAPEALWDSVTQKVFTLPDE
                     TLLFAGHERCARAVSTVQEQRRWHPFFSGLTRDAFLAHIAALPELASDTPTF"
     gene            983..2419
                     /gene="ccoG"
                     /locus_tag="PLB25_17535"
     CDS             983..2419
                     /gene="ccoG"
                     /locus_tag="PLB25_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HOE43412.1"
                     /translation="MTNATRDGKKVIPIAVAEPDEEMISLYAAHKKIYPRSVKGVFSN
                     WRWIMVWVTQIIFYGLPWLEWGQRQAVLFDLSVRRFYIFGLVLYPQDFIYLTGMLIIA
                     ALSLFLFTAVAGRLWCGYACPQTVYTEIFLWIEKLVEGDRAARIRRDEGAFSAEKLWR
                     KTGKHFLWIVFSLWTGFTFVGYFVPIRDMGGLLLTMNLAAWEIFWVFFYGFATYGNAG
                     FMREQVCLHMCPYARFQSAMFDKDTLIVTYDTERGEPRGARSKKADPAKLNLGACVDC
                     SLCVQVCPTGIDIRNGLQYQCIGCGACADVCDTVMDKVGYPRGLVKYSTENAMANHWT
                     QKQTVRHVFRPRVLVYTSILTTIVLAIFISLAMRTPFKVDVVRDRGVMARVVEAGTTE
                     NVYRLQIMNATEADQRYKISVSGLPKLLISSDTTFKVESTKAITVPVRVQLPFEGAAP
                     GSHVIHFDIESLDSPGKLLEKSTFIVPQ"
     gene            2551..3324
                     /gene="surE"
                     /locus_tag="PLB25_17540"
     CDS             2551..3324
                     /gene="surE"
                     /locus_tag="PLB25_17540"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HOE43413.1"
                     /translation="MKILISNDDGFQAPGIVALFEALKDVAEVEVVAPEQNNSAKSNA
                     LTLHSPLYVRRADNGFRFVNGTPADCVHIALTGLLDYRPDLVVSGINNGANMGDDTIY
                     SGTVGAAMEGYLFGIPSIAFSQVERDWHHLDAAASKARQLVLQMTKQNLVGGSPWLLN
                     VNIPNLPLHQIGHIKLCRLGRRHAAEAVITQQSPRGETMYWIGAAGPAKDDADGTDFH
                     ATAQGHIAMTPLKVDLTDHDHLGYWAQTATRLAAGMGQA"
     gene            3423..>3779
                     /locus_tag="PLB25_17545"
     CDS             3423..>3779
                     /locus_tag="PLB25_17545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HOE43414.1"
                     /translation="MSKPTQTRHHPPQGVGMDSSAVRQSMVTKLAQQGLSDAQVLLAM
                     GMVERHRFVDSALVNQAYEDTSLPIGLGQTISKPGVVARMLQLLRQGSSGRLGRVLEI
                     GTGCGYQAAVLSHLCTE"
BASE COUNT          741 a         1023 c         1135 g          880 t
ORIGIN      
        1 ggctgctgat tggcgacgac cagcaggcat gatgctggat gcctcagtcc aacacaagcg
       61 gacaactgaa gaatcatggt tcgtgattgg ctgatgcgcg ccttcaagca ggcggccgaa
      121 cagatcgtga atgcgactga cagacctgcc tgtcagcctg aaaactctgt caatagtggc
      181 agtttgtcac tgaatgctgt ggcagcgacg cacttctatt cttgtaaatc gttgatttta
      241 ttgacggttt tttttggctc cgattggcat gaatcttgat gtttcgcttg tgctgacgcg
      301 ctcgatgatg ctgggcgttc cacaaggagc acgatgtatt tccgaatttt gaatgacgat
      361 gcaagtggtg aattgaccta tctgctggcc gacctgtcgg ggcgcgaggc ggtgctgatt
      421 gacccgcacg gccgggacct gccggttttg tcggccttgt tgtctgatcg ggatttgcgc
      481 ctgcgctggg tcttgcgcac ccaccatcac gatgaccggc aaacccggga gcctgagttg
      541 ctggcccgtc ttgaggcacc catggttcag ggagatgcgt caagagacac cccggttgtc
      601 ggcgacggca cggtattgcc ctttggtgac gaactgattc atgtggtggc caccccgggg
      661 cacacgccgc attgcctgag ttttgcctgg cgggatcgct tcttttgtgg tgccttgctg
      721 gcggtgacgg cttgcccgca ccaggcccgc ccacaagcgc cagaggcctt gtgggacagt
      781 gtgacgcaaa aggtttttac actcccggat gaaacgctgt tgtttgccgg ccatgagcgc
      841 tgcgcacggg ctgtgagcac cgtccaggaa cagcgccgct ggcacccctt tttttcgggt
      901 ctgacacgcg acgcatttct tgcgcacatc gcagccctgc ccgaacttgc atcagatacc
      961 ccgacttttt gaggaaattg acatgactaa cgccaccaga gacggcaaaa aggtgattcc
     1021 gattgccgtg gccgagccag acgaagagat gatttcgctg tacgccgcgc acaagaaaat
     1081 ttacccgcgc agtgtgaagg gcgtgttttc caactggcgc tggatcatgg tctgggtcac
     1141 gcagatcatt ttttacgggc tgccctggct tgaatggggc cagcgccagg cagtcttgtt
     1201 tgatttgagc gtgcggcggt tttatatttt tggtctggtg ctctacccgc aagacttcat
     1261 ttacctgacc ggcatgctga tcatcgcggc gctgtcgctg tttttgttca cggcggtggc
     1321 cgggcggttg tggtgcggtt acgcctgccc acaaacggtt tataccgaga tattcttatg
     1381 gattgaaaag ctggtggaag gtgaccgggc ggcacgcata cgccgcgacg agggcgcttt
     1441 ttccgctgaa aagctgtggc gcaagactgg caagcatttc ctgtggattg tgttttcgct
     1501 gtggaccggc tttacctttg tcgggtattt tgtaccgatc agggatatgg gcggtttgct
     1561 tctgaccatg aatctggctg cctgggaaat tttctgggtt ttcttctacg gttttgccac
     1621 ctatggcaat gccggcttca tgcgcgagca ggtgtgcctg cacatgtgtc cgtatgcacg
     1681 tttccagagc gccatgtttg acaaggacac cttgatcgtg acctatgaca ccgagcgtgg
     1741 cgagccgcgc ggtgcccgct ccaaaaaggc cgatccggcc aagctcaatc tgggtgcctg
     1801 cgttgattgc agcttgtgcg tgcaagtctg cccgaccggg attgatattc gaaatggcct
     1861 gcagtaccag tgcattggtt gcggtgcctg tgccgacgtg tgtgacacgg tgatggacaa
     1921 ggtgggctat ccgcgtggtc tggtgaagta ctctaccgag aacgccatgg caaaccactg
     1981 gacgcaaaag cagaccgtgc gccatgtgtt tcggcctcgg gttctggtct ataccagcat
     2041 cttgaccacc atcgtgctgg ccatctttat cagtctggcc atgcgcacgc cgttcaaggt
     2101 ggatgtggtg cgtgatcgcg gcgtgatggc acgcgtggta gaagctggaa ccaccgaaaa
     2161 tgtttaccgg ctgcagatca tgaacgcaac cgaggctgac cagcgttaca agatttcggt
     2221 gagtggtttg ccaaaactgc tgatcagttc cgataccacg ttcaaggtcg aatccaccaa
     2281 ggcgattacc gtgccggtgc gggtgcagtt gccgtttgaa ggggcagcgc cgggttccca
     2341 tgtgatccat ttcgacattg aatctctcga cagcccgggc aagctgctcg aaaaatcaac
     2401 cttcatcgtt cctcagtagc ttggccttgc cgcgaaccag accgccggat gccttggtcg
     2461 gggtctgcgc cgctggtctc gcggctttgc gttgttggat tggctggcaa agatgtggca
     2521 cctacaattc aggtcaaaat atgccgcatg atgaaaattc ttatatccaa cgacgatggc
     2581 tttcaggcac cgggcattgt ggccttgttt gaagcgctca aggatgttgc cgaagttgaa
     2641 gtcgtcgcac ccgagcaaaa caacagtgcc aaatccaatg cgttgacgct gcactcaccc
     2701 ctttatgtgc gccgcgccga caacgggttt cgctttgtca atggcacccc ggccgactgt
     2761 gttcatattg cgctgaccgg tttgctcgat taccggccgg atctggtggt ctcgggtatc
     2821 aacaacgggg ccaatatggg tgacgacacc atttactctg gcacggtggg tgcagccatg
     2881 gaaggctatt tgtttggcat cccgtcgatc gcgttctcgc aagtcgagcg cgattggcac
     2941 catctggacg cggcggccag caaggcacgc cagttggtgt tgcaaatgac gaaacagaac
     3001 ctggtgggcg gaagtccctg gctgctcaat gtcaatattc cgaatttgcc tttgcatcag
     3061 attggccata tcaaactgtg ccgactaggc cgcaggcacg cggctgaagc ggtcatcacc
     3121 cagcaaagcc cgcgcggcga aaccatgtat tggatcggtg cggccggtcc ggccaaagac
     3181 gatgccgatg gcaccgattt tcatgccacg gcacaggggc atattgccat gaccccgttg
     3241 aaggttgatt tgaccgacca tgatcatctg ggttattggg cacagacagc caccaggctt
     3301 gccgccggta tgggtcaagc ctgatgcctc acaacgcaag ccccaaccag aaaacgcgcc
     3361 caggttttcc ggccagactc gcgccgctgg tgccgcccgc caaagtgacg gttgccaatc
     3421 gcatgtccaa gccgacacag acccggcatc atccccctca gggtgtgggt atggattcaa
     3481 gcgcggtccg gcaaagcatg gtgaccaaac tggcgcagca gggactttct gatgcccagg
     3541 tgctgctggc aatgggcatg gttgagcgac accggtttgt cgatagtgcg ctggtcaacc
     3601 aagcgtacga agataccagt ttgccaatcg gcctgggcca aaccatctcc aagccaggcg
     3661 tggtggcacg catgctgcag ttgctgcgcc agggcagtag tgggcgattg ggccgggtgc
     3721 tggagatcgg caccgggtgc ggctaccagg cagcggtttt gagccatctt tgcaccgag
//
LOCUS       DAOSLL010000490         3779 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_21307_length_3779_cov_4.804780, whole genome shotgun sequence.
ACCESSION   DAOSLL010000490 DAOSLL010000000
VERSION     DAOSLL010000490.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21307_length_3779_cov_4.804780"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2055
                     /locus_tag="PLB25_17550"
     CDS             <1..2055
                     /locus_tag="PLB25_17550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003052418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HOE43415.1"
                     /translation="LGDIGPLAGKPVMEGKGCLFKKFADIDVFDIELAERDPDKLIEI
                     IAAMEPTLGGINLEDIKAPECFYIEQKLSARMGIPVFHDDQHGTAIISSAALLNGLEL
                     VGKSIGDVRVAVSGAGAAAIACLNVMVGLGVQVKNVFVCDSKGVIYQGRPGGYDESKA
                     RFAQDTPLRTLADACNGADVFLGCSAAGVLTQDMVKTMATQPIILALANPEPEIRPEL
                     AKAVRPDCIIATGRSDYPNQVNNVLCFPYIFRGALDCGATKITEEMKLACVHEIAALA
                     KADISEEVASAYAGQELAFGVDFIIPKPFDSRLILRIAPAVAAAAERSGVAARPIKDM
                     DAYRRTLLRFVYSTGMLMEPVFNAARKAPLTSKRVAYAEGEDERVLRAVQVVVEGGMA
                     RPILIGRPAVIEARIDKAGLNLQLGRDFDCCNPEEDPRFRQYWETYHQIMGRQGVTPQ
                     AAKAAVRRSTTIIAALMVKLGHADAMLCGMHGRFDAHMNHVRNVIGLRPGVSVLATLN
                     ALMLEHHSLFVADTYVNDDPTAEQLAGIARMAADEVRRFGLPPKVAFLSHSNFGSSNR
                     PSALKMRTARDLFKVACPGVECEGEIRGDAALSEDIRHKSLLESTLSGSANILICPNL
                     DAANILFNVLKMTVSQGVTVGPILLGSAAVAHVLSLSSDSHRVVNMTALAVADVNAVR
                     VI"
     gene            complement(2065..3690)
                     /locus_tag="PLB25_17555"
     CDS             complement(2065..3690)
                     /locus_tag="PLB25_17555"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE43416.1"
                     /translation="MNLSNLKISAKLLFGFSLIVLLTSLTGGLSIYQMGLINNATAEL
                     SENWLPDIALGSELQGLLNDMRRAELQHVVAVNADEKKAEGARIVANIAKLGDAARRL
                     ESRLDSPPQRQFFDKYKQEIAAYLLTSARLIELSLVGSSGLEATTKYLKGDSRTTFRA
                     IFKTLSDLGGISTKGAEDAAAQAHQTYYSAKINVALGLMVAIAMAAFLGFWISRQITR
                     PIQVAVGAATQFAAGNLTETLKADGTDEPAQLLRALESMRVDLSKVVASVRAGSDSVA
                     TASSEIAQGNSDLSARTESQASALEQTSAAMEQLGSTVAQNAESARKANDLAVGASGV
                     AMQGGEVVGQVVETMKGINDSSRKISDIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVASEVRSLAGRSAEAAKEIKALISASVERVAQGTALVDKAGDTMAEVVNAIKQV
                     TDIMGEISNASSEQSIGVAQVGEAVSQMDQATQQNAALVEEMAAAAGSLRSQAQELVQ
                     TVSVFKLADAQQLGAQPATLGMRPVSARPLRLA"
BASE COUNT          702 a         1162 c         1131 g          784 t
ORIGIN      
        1 ctgggcgaca tcggcccgct ggccggtaag ccggtgatgg agggcaaggg ctgcctgttc
       61 aagaagttcg ccgatatcga tgtttttgac attgaactcg ccgaacgtga cccggacaag
      121 ctgatcgaga tcatcgctgc catggagcca acgctgggcg gcatcaacct ggaagacatc
      181 aaggcccccg agtgcttcta tattgagcaa aaactcagtg cacgcatggg cattccggtg
      241 tttcacgacg accagcatgg cacggcgatc atttccagcg cggcattgct caacggtctg
      301 gaactggtcg gcaagtcaat tggggatgtg cgcgtcgcgg tgtcgggggc cggtgctgcc
      361 gcgattgcct gcctgaacgt catggtcggg ctgggggtgc aggtcaagaa tgtgtttgtc
      421 tgtgactcca aaggcgtgat ttaccagggt cgccccggtg gttacgacga gtccaaggcc
      481 cgctttgccc aggacacccc gctgcgcacg ctggccgatg cctgcaacgg cgcggatgta
      541 ttcctgggct gctcggcagc cggcgtgctg acgcaggaca tggtcaagac catggccacg
      601 cagccaatca tcctggcgct ggccaacccc gagcctgaga tccgccctga gctagccaag
      661 gcagtgcggc ctgactgcat cattgccacc ggccgttcgg actacccgaa ccaggtcaac
      721 aatgtgctgt gttttccata catctttcgt ggcgcgcttg attgcggcgc caccaagatc
      781 accgaagaga tgaagctggc ctgtgtgcat gaaattgccg ccctggccaa ggctgacatc
      841 agcgaagagg tggccagcgc ctatgccggg caggaactgg cttttggggt ggacttcatc
      901 atcccgaaac cctttgactc acgcctgatt ctgcgcattg ccccggctgt tgctgctgca
      961 gccgagcgct ccggcgtggc ggcccgtccg atcaaggaca tggatgccta ccgccgcacc
     1021 ctgctgcgct ttgtctattc cactggcatg ctgatggagc cggtgttcaa tgccgcacgc
     1081 aaggcaccct tgacttccaa gcgcgtcgcc tacgccgaag gtgaagacga gcgggtgctg
     1141 cgggcggtgc aggtcgtggt tgaaggcggc atggcgcgcc cgatcctgat tggccgcccg
     1201 gcggtgatcg aagcgcgcat tgacaaggct ggcctgaacc tgcaactcgg tcgcgacttt
     1261 gactgctgca accccgaaga agacccgcgt tttcgccagt actgggaaac ctaccaccag
     1321 atcatgggac gtcaaggtgt caccccgcag gcagccaagg cggcggtgcg ccgcagcacc
     1381 accatcattg ccgcgctgat ggtcaaactc ggtcatgccg atgccatgtt gtgcggcatg
     1441 catgggcgct ttgacgcgca catgaaccat gtgcgcaacg tgattggttt gcggcccggc
     1501 gtttcggtgt tggccacgct caacgcgctg atgcttgaac accactcgct gtttgtcgcc
     1561 gacacctatg tcaacgacga cccgaccgcc gagcaactcg caggcattgc ccgcatggca
     1621 gcagacgaag tgcgccgctt tggcttgccg cccaaggtgg catttctgtc gcactcgaat
     1681 tttggctcat ccaaccgccc gtcagccctc aagatgcgca ccgcgcgcga tctgttcaaa
     1741 gtcgcctgtc cgggggtcga atgcgagggc gaaattcgtg gcgatgccgc cctgtcagag
     1801 gatatccggc acaagagtct gctggaatca accttgagcg gatcggccaa tattctgatt
     1861 tgcccgaatc tcgatgcggc caatatcttg ttcaacgtgc tgaagatgac cgtcagccaa
     1921 ggcgttactg tcgggcccat cctgctgggc tcggcggcgg tggcccacgt gctgtcgctg
     1981 tcatccgact cgcaccgggt ggtcaacatg accgccctgg cagtcgccga tgtcaacgcg
     2041 gtgcgggtga tctaaccgag ccagctaggc caggcgaagc ggcctggcgc tgaccggtcg
     2101 catccctagc gtggcaggtt gcgcgccgag ctgctgcgca tcggccagct tgaacaccga
     2161 cacggtttgc accaattcct gtgcctggct gcgcaggctg ccagccgcag cagccatctc
     2221 ttccaccagg gcggcgtttt gctgggtcgc ctggtccatc tggctgaccg cctcgccaac
     2281 ttgcgcgaca ccgattgatt gttcgctgct ggcattgctg atttcaccca tgatgtcagt
     2341 gacctgtttg atcgcgttga caacttcggc catggtgtcg ccggctttgt cgaccaatgc
     2401 ggtaccttgc gcaacccgct ctacgctggc gctgatcagc gccttgattt ccttggccgc
     2461 ttcggcgctg cgacctgcca gactgcgcac ctcgctggcc accacggcaa agccacgccc
     2521 ttgctcaccg gcccgagccg cttccacggc ggcattcaag gccagaatat tggtctgaaa
     2581 cgcaatgccg tcaatgacgc tgatgatgtc cgatatcttc ctggaagagt cattgattcc
     2641 cttcatggtc tcgaccacct ggccgaccac ctcgccaccc tgcatcgcca cgcctgacgc
     2701 gccgacggcc agatcgttgg ccttgcgcgc cgattctgcg ttttgcgcca cggtagagcc
     2761 gagttgctcc atcgcagccg aggtttgctc cagcgcactg gcctggcttt cagtgcgggc
     2821 cgacaggtcg ctattgccct gggcgatttc agagcttgcc gtggctacgc tgtcggaccc
     2881 ggcacgcaca cttgccacca ccttggacaa atcaacgcgc attgactcca gtgcccgcag
     2941 caactgcgct ggctcgtcag tgccgtcggc tttcagcgtc tcggtcaggt tgccagcggc
     3001 aaattgcgtg gcggcaccga cagcaacctg aattggccgg gtgatctggc gtgaaatcca
     3061 gaagcccaag aatgccgcca tggcaatcgc caccatcagc cccagggcca cgttgatctt
     3121 ggcgctgtaa taagtttggt gcgcctgggc ggcagcatct tcagcgccct tggtgctgat
     3181 gccaccgagg tcactgaggg ttttgaaaat ggccctgaaa gttgttctcg agtcaccttt
     3241 caggtatttg gtggtggctt ccagacctga cgagccgacc aatgagagtt cgatcagcct
     3301 ggcgctggtg agcaggtagg ccgcgatctc ctgtttgtat ttgtcaaaga attgccgctg
     3361 gggtggcgaa tccagccgcg actccagcct gcgggcggca tcacccagtt tggcaatgtt
     3421 ggcgacgatg cgagccccct cggccttttt ttcatcagca ttgaccgcca ccacgtgctg
     3481 caactcggca cggcgcatgt cgttcaggag accctgcaac tcacttccga gggcgatgtc
     3541 cgggagccag ttttctgaca actcggccgt cgcgttattg atgaggccca tctgataaat
     3601 ggacaaacca ccggtcaggg aagtcagcaa cacgataagg gagaagccaa aaagcaattt
     3661 ggcactgatt ttgaggttgg agaggttcat gacggtcgtt gggttaacag gttacaagcc
     3721 accaaaggat gtggtcagaa ataacttccc ctatttgcac cggcggattt gggcgcaca
//
LOCUS       DAOSLL010000491         3774 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_21342_length_3774_cov_4.756386, whole genome shotgun sequence.
ACCESSION   DAOSLL010000491 DAOSLL010000000
VERSION     DAOSLL010000491.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3774)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3774
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21342_length_3774_cov_4.756386"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            79..2874
                     /locus_tag="PLB25_17560"
     CDS             79..2874
                     /locus_tag="PLB25_17560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE43417.1"
                     /translation="MFPVAQLDPSARLRAMLDNVSAMIGYWDKDLRNQFGNRAYATWF
                     GIDPQRMRGMHIRELLGEQRFELNRPYIEAALRGERQVFERAIPTPGGGQIRHSLAEY
                     IPDLANGEVQGFFVQVSDISPVKEAQAALQASEERLRGLFELSPLGIGLCSLDGRFIE
                     VNEALCRICGYTGAELKELDYWALTPEDYAPAELLQMGTLLRTGRYGPYDKEYRHKDG
                     HRVAVQLNGMLVTGADGQQRIWSMVEDIGPRRGRFMELIQAKEMVEVANRAKSAFLAA
                     MSHEFHTPMNAVLGLLKILKGTPLSLKQLGEVEQSETAARSLLALLDEILEFCSVESD
                     PLTPVVQPFQLDQLLRELAPELAANGLSDSVELLFDLQPRLPPVVSGDLWRLKQVLTH
                     LCSNAVKFTRAGEVVVSVRLEGQQGNVAELEFAVSDTGCGIAANRQQRIFEAFTQSET
                     PTRRSLGGAGLGLAISRRLVALMGGTLKVQSTLGAGSKFYFSLPMPVVLPVPPDLQLP
                     PSSLPAPLEVLVVDDKAVARRLILGQLQARGLVATGVDSGAAALQWLQQHCQRSTSPF
                     TVVLLDWHMPGMDGWETARKIKALDFGVCRPPRLVMVGANGRDQLADRTEAEQSRVSG
                     FLAKPFSPEMLLDVVAQAGVDDLPGPNPPADGQPGLANMRILVVEDNLVNQMVAKELL
                     TREGAQVSLADNGQLGVDAVASADPPFDVVLMDLQMPVLDGFAATRVIRQQLGLARLP
                     VVALSANALPDDIAQALAAGMNGHVGKPIDLPKLVALVRRLVGLAQSRAQQIQAAQPA
                     QRITDALVIDLQQLGKINFARALDQVGGDTTLYRRFALGFVQDTADFADRLALHVAHD
                     EPKDAMRVMHTLKGLSATIGADCLADFAARKETELKQAPLEPVARGVLVQQTRDGITA
                     VSAAALQIVKTIDATLD"
     gene            2954..3237
                     /locus_tag="PLB25_17565"
                     /pseudo
     CDS             2954..3237
                     /locus_tag="PLB25_17565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014698.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            3415..>3774
                     /locus_tag="PLB25_17570"
     CDS             3415..>3774
                     /locus_tag="PLB25_17570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HOE43418.1"
                     /translation="MNQPESDADDLNFMTTRSVARQLGLAVRSVQLMVDRGELEAWRT
                     PGGHRRIARQSVQKWQTRGSDDRLQGLVDGSKLPADFMPSQGAALVSTQNGTVLLIED
                     SIHFQNLVKLLLNQEFPD"
BASE COUNT          756 a         1075 c         1189 g          754 t
ORIGIN      
        1 acgcgagcac gcgccgggca gccgtgcggc ggcagattac gcacaagtgg tacaggcgct
       61 tgaaaggctc tgggcgtgat gtttccggtt gcccagctcg accccagtgc gcggctgcgc
      121 gccatgctcg acaacgtctc ggcgatgatt ggctattggg acaaagacct gcgcaaccag
      181 tttggcaacc gggcctatgc aacctggttc ggcattgatc cgcagcggat gcggggcatg
      241 cacatcaggg aactgctggg tgagcagcgt ttcgaactga accggccgta tattgaagcg
      301 gcactgcgcg gtgaacggca ggtgttcgag cgggccattc caacacctgg gggtggccag
      361 atccggcatt ctctggcgga gtacatcccg gacttggcaa acggcgaagt ccagggtttt
      421 tttgttcagg tttccgacat ctccccggtc aaagaggccc aggcggccct gcaggccagt
      481 gaagaacgcc tgcgcggcct gtttgagctg tcgccgctgg gcatcgggct gtgctcgctt
      541 gatggtcgct tcattgaagt caatgaagcg ctgtgccgga tctgcggtta caccggggca
      601 gaactcaaag agcttgatta ctgggccttg acgcccgaag actacgcgcc cgcagaactg
      661 ctgcagatgg gcacgctact cagaaccgga cgctacggtc cctacgacaa ggaataccgc
      721 cacaaggacg ggcatcgggt tgccgtgcag ttgaacggca tgctggtgac aggcgccgat
      781 ggtcagcaac gtatctggtc gatggtggag gacattggcc cgcgccgcgg caggtttatg
      841 gagctgattc aggccaagga aatggtcgaa gtagccaacc gtgccaagag cgcgtttctg
      901 gctgccatga gccacgaatt tcatacaccc atgaatgcgg tactgggatt gctcaagata
      961 ctcaagggca cgccactgag cctgaagcag ctcggcgagg tcgaacaatc cgaaaccgca
     1021 gccagatcac tgttggcctt gctcgacgag attctggagt tttgcagtgt tgaatccgac
     1081 ccgctgacac cagtggtgca accgtttcag ttggaccagt tattgcgtga actggcgcca
     1141 gagctggcag ccaacgggct ttctgattca gtggaactgc tgtttgacct gcaacccaga
     1201 ctgccgccag tggtgtctgg cgacctctgg cgactcaagc aggtgctgac ccatttgtgc
     1261 agcaatgctg tcaaatttac cagagctggc gaagtggtgg tgtcagttcg gctggaaggg
     1321 cagcaaggca atgtcgcgga acttgagttc gccgtgagtg acaccggctg cggcattgcg
     1381 gccaatcggc agcagcggat ttttgaggcc ttcacccaga gcgagacgcc cacccggcgc
     1441 agcctgggcg gcgccggcct gggcctggcc atcagtcggc ggctggtggc actgatgggc
     1501 ggcacgctga aggttcaaag caccctgggt gcgggcagca agttctattt cagtttgccg
     1561 atgccggtgg tgttgccggt accgccagac ctgcagttgc caccgtcaag cttgcccgcg
     1621 ccactggagg tgctggtggt tgatgacaaa gccgtggcgc gtcgtctgat actgggccag
     1681 ttgcaggcca gggggttggt ggccactggc gttgacagcg gcgcggcggc actgcaatgg
     1741 ctgcaacagc actgtcagcg gtcgaccagc ccgttcactg tggtgctgct ggactggcac
     1801 atgcccggga tggacggctg ggaaaccgcc cgcaagatca aggccctgga ctttggcgtt
     1861 tgccgcccgc cgcgcctggt catggtgggt gccaatggcc gcgaccagtt ggccgaccgg
     1921 accgaagccg agcagtcgcg cgtcagcggt ttcctggcca aaccgttcag cccggagatg
     1981 ttgctcgatg ttgtggccca ggccggtgtc gatgatttgc caggcccgaa cccgcccgcc
     2041 gacgggcagc cgggtctggc aaacatgcga atcctggtgg tggaagacaa ccttgtcaac
     2101 cagatggtgg caaaggaact gctcacccgc gagggcgcgc aggtgtcgtt ggcagacaac
     2161 ggtcaactgg gcgtggacgc tgtggccagc gcggacccac cgtttgacgt ggtgctgatg
     2221 gacctgcaaa tgccggtgct cgatggtttt gccgccacgc gcgtgattcg ccagcaattg
     2281 gggctggcgc gattgccggt tgttgccctg agcgccaatg ccctgcccga cgacatcgcc
     2341 caggcgcttg ccgcaggcat gaacggccat gtcggcaaac ccattgatct gcccaagctg
     2401 gtggccttgg tgcgccgcct ggtgggtctg gcgcagtcga gggcgcagca gatccaggcc
     2461 gcccagccag cgcaacggat caccgatgcg ttggtcatcg atttgcagca actgggcaaa
     2521 atcaatttcg ccagggcact cgatcaggtt ggtggcgaca ccaccctgta ccggcgtttt
     2581 gcgcttggct tcgtacagga cacggccgac ttcgccgacc ggctggcgct gcatgtcgca
     2641 catgacgagc caaaggacgc gatgcgcgtc atgcacacgc tcaaaggtct gtcagccacc
     2701 atcggtgccg actgcctggc cgattttgct gccaggaaag agaccgaact caagcaagcc
     2761 ccgctggaac ccgttgcgcg cggcgtgctg gtacaacaaa ctcgcgacgg cattaccgcc
     2821 gtcagcgctg cggctttgca gatcgtgaaa accatcgatg ccactcttga ctgattcaac
     2881 ggccatgaag cagcacaccg atcctgtctc gctggccaag gtgtctggcc tggcaccagc
     2941 cgatgcgctg gggctagtgc tggaaaacgt cgaggatgcg attgtgaaga tgacggcgat
     3001 caaacagatg ggtatccttt ttgccatgga cgattttggc accggctatt cctcgctgtc
     3061 ctatctggct cgcctgccgc tttgtcgatg cacgtcattg cggagggggt ggaaaccgaa
     3121 gcacagcgcc tgtttcttca tcaacatggc tgccatgcct accagggtta tctgtacagc
     3181 cgcccattgc agcttgaggc gctggatgag tttctgaatc acgcaacaag ttgttgacaa
     3241 atcagcgcat ggtggaaaac tcagacacca ccaagccggt gcagtttgtt aaacccaacg
     3301 ttggctccaa aagccttgtc aactggcctg gcaattggta taaaacatca tttcattgat
     3361 aaaagtgata aacgtatcag ggggaatagg ctgatttttc tataaaatga aagcgtgaac
     3421 cagccagaat ccgacgccga cgacctcaac ttcatgacca cccgatcggt tgcgcgccag
     3481 cttggactgg cagtgcgatc agtgcaactg atggtggatc ggggcgaact ggaggcctgg
     3541 cgcacgcccg gcgggcatcg gcgcattgcg cgccaatcgg ttcaaaagtg gcaaacgcgc
     3601 gggtcggatg atcgtttgca gggcttggtc gatggttcaa aactgccggc cgacttcatg
     3661 ccttcccaag gcgcagctct agtgtccaca cagaatggca ccgtgctctt gatcgaggat
     3721 tcgattcatt ttcagaattt ggtcaaattg ctcctgaatc aagaatttcc cgat
//
LOCUS       DAOSLL010000492         3773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_21349_length_3773_cov_4.616729, whole genome shotgun sequence.
ACCESSION   DAOSLL010000492 DAOSLL010000000
VERSION     DAOSLL010000492.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21349_length_3773_cov_4.616729"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            3..1964
                     /locus_tag="PLB25_17575"
     CDS             3..1964
                     /locus_tag="PLB25_17575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE43419.1"
                     /translation="MVTAAAHGDLSKRIELDDKHGFAKDLSMHVNQLADETMRIKKAL
                     DNASTCVMIADAQGSIIYLNTSMTDLLQGAENDIRQQLPQFRAGEVLGSSFSRFHAST
                     GQADLLNSLSGNYKADITLGGRVFGLVATPVFDDNQHRLGSIVEWRNRNDEIKADAEA
                     RANTRIKHALDKCTTNVMIANASHDIVYMNETAQLMMQRNEAELRKSLPHFDASKLLG
                     TNIDLFHKEPNRQRLTLAALQSTHRAEIRVGTLCFGFIANPIVDATGKRVGTVVEWLD
                     RTAEVGVEGEIASIVQGAAQGDFSKRLMLAGKSGFFENLSGNINQLMDASEKGLSDVA
                     HVLLAFAEGDLTQRMDGNFGGLFGKVKDSANTTAENLTRVLGEAHAAAAALTGAANQV
                     SATAQSLSQAASEQAASVAETSATMDTMSASITHNSDNARVTDAMANKASKEAVDGGK
                     AVNATVLAMKQIAAKICIVDDIAYQTNLLALNAAIEAARAGEHGKGFAVVAAEVRKLA
                     ERSQQAAREIGELADKSVSTAEHAGKLLDEIVPSIQKTSELVQEISASSTQQSESVVQ
                     ISGAMGQLERATQQNASASEELAATSEELSGQAEQLQQSISFFKTGEVSARKSELAAP
                     APERRKQASPSKVTVARVRNDSAGSFRPY"
     gene            complement(2149..2349)
                     /locus_tag="PLB25_17580"
     CDS             complement(2149..2349)
                     /locus_tag="PLB25_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43420.1"
                     /translation="MNRYFASAMFDKPIRYVARPVRPAVLARFFGTLAISWIPVLSAD
                     LPQVLVNPGRPAVGHLAMLHLI"
     gene            2769..3698
                     /locus_tag="PLB25_17585"
     CDS             2769..3698
                     /locus_tag="PLB25_17585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOE43421.1"
                     /translation="MREVDRVIAQRLDSGVPLVGQVSRYIISAGGKRLRPVLLLLICG
                     ALGFKGVQRFNLAAVVEFIHTATLLHDDVVDDSSLRRGNATANETFGNPASVLVGDFL
                     YSRAFQMMVDADNMRIMQVLADATNIIAEGEVMQLMNMHNAALDEVGYLQVIRSKTAK
                     LFEASARVGAILAGASHEIEACCADYGQAMGTAFQVIDDVLDYTGDVSVMGKNLGDDL
                     REGKTTLPLIAAMQRGDAAQRELIKVAIETGDVKMIKQVVAIVQSSGAIDAARRAAML
                     EAERAAAAASQLPEGPHTACLLQLATQLLERQS"
BASE COUNT          857 a         1051 c         1121 g          744 t
ORIGIN      
        1 cagtggtaac agccgctgca catggcgact tgtccaaacg tatcgagctt gatgacaaac
       61 atggctttgc caaagatctg agcatgcatg tgaaccagtt ggcagacgag accatgcgca
      121 tcaaaaaagc gctggacaac gcttccacct gcgtgatgat cgccgatgcc cagggcagca
      181 tcatctacct gaacacgtcg atgactgacc tgttgcaagg tgccgagaac gacatcagac
      241 agcaacttcc gcagtttcgc gcaggtgagg ttttgggcag cagttttagc cggttccacg
      301 ccagcaccgg gcaagcggac ttgctcaata gcctgagtgg caattacaag gctgacatca
      361 cgctgggtgg cagggtgttt ggcctggttg ccacaccggt gttcgacgac aaccagcatc
      421 ggctgggctc aattgtggag tggcgcaacc gcaatgatga aattaaggcc gacgcggagg
      481 ctcgcgccaa tacccgcatc aagcatgcgc tggacaagtg cacaacgaat gtgatgatcg
      541 ctaacgccag ccacgacatc gtttacatga acgagacggc gcagctcatg atgcagcgca
      601 acgaggcaga gttgcgcaag agcctgcccc actttgacgc gtcgaaattg ctgggcacga
      661 atattgacct ctttcacaag gagccaaatc gccagcgctt gacgctggct gccctgcagt
      721 ccacccaccg ggctgagatc cgggttggca cgctgtgttt tggcttcatc gccaacccga
      781 ttgttgatgc caccggaaag cgcgtgggca cggtggtcga gtggctcgac cgcaccgccg
      841 aggtcggcgt ggaaggtgag atcgcctcga tcgtgcaggg tgcagcccaa ggtgatttca
      901 gcaagcggct gatgcttgcg ggcaagagcg gcttctttga aaatctgtcg ggcaacatca
      961 accaactgat ggatgccagc gaaaaaggct tgagtgatgt tgcccacgtg ctgctggcct
     1021 ttgccgaagg tgacctgacc cagcgcatgg acggaaattt tggcgggctt tttggcaaag
     1081 tcaaagacag cgccaacacc accgccgaga atctgacgcg cgttttgggc gaagcgcatg
     1141 ccgctgccgc tgccctcacc ggcgctgcca atcaggtcag cgccacggcg cagtccttgt
     1201 cacaggcggc cagcgaacaa gccgccagcg tggcagaaac cagcgccacg atggacacga
     1261 tgtcagcatc catcacccat aacagcgaca acgccagggt taccgatgcc atggccaata
     1321 aagcaagcaa ggaagccgtt gacggtggca aagcggtcaa tgccacggtg ctggccatga
     1381 agcagattgc cgcaaaaatc tgcatcgttg atgacatcgc ctaccagacc aatctgttgg
     1441 cactcaatgc ggccatcgag gcggctcgcg ccggagagca cggcaagggc tttgcggtgg
     1501 ttgcagccga agttcgcaag ctggccgagc gcagccagca agctgccaga gaaatcggcg
     1561 aactggccga caagagcgtt tctaccgccg aacacgcggg caagctgctc gacgaaattg
     1621 tccccagcat tcaaaagacc agcgaactgg tgcaggaaat ttccgcgtcc agtacccagc
     1681 agagcgagtc agtggttcag atcagtggtg ccatgggcca actcgaacgg gccacgcagc
     1741 agaatgcctc ggcctccgaa gagctggccg ccaccagcga agagctgtcc gggcaagccg
     1801 agcagttgca gcagagcatt tcgtttttca agacaggcga ggtttcggcc cggaagtccg
     1861 agcttgcggc accagccccg gaaaggcgca agcaggcaag cccatcgaaa gtgacggtag
     1921 ctcgggttcg caatgattcg gctggcagtt tccggcccta ctgacgggtg tcaatctcga
     1981 tgacagacgg gagcgatggg atatgggtca gcttttcagc atcatcggaa ttgagcagaa
     2041 tttgaaccct cagcctggtc gcgatacagc aagaccatcg cggcaacgat tgctgccttt
     2101 tttatatgaa ttttgtggct ccagccctta tggatggggc gctaccagct atattaaatg
     2161 cagcatcgca aggtgcccga ctgcaggtct tcccgggttg accagtactt gcggcaagtc
     2221 ggcggacagc acgggaatcc atgatattgc cagcgtgccg aaaaacctgg ccagcaccgc
     2281 cggtcgcacc gggcgcgcaa catagcgaat cggcttgtca aacatggccg aagcaaagta
     2341 gcggttcatc ccggccggag gggccaaaaa tcccgtcttt actgtccatg caaacgtcaa
     2401 tccaccagcc tctgaggcga caccctctgg ctgcggccaa tcgccgagtt aggcagttgg
     2461 actggtcaaa gccgcccaat tgtcccggag caactctatt gaatcaccct gctttttgaa
     2521 gctgatgtga acagcaaccc agggctggaa agtctgacac gctactgatc catcgctgca
     2581 tgtgttgtca aggtttgcac gaaatggcat agtgccgcgc agagttggca gcatcttgcg
     2641 ccaacatcga cgttattcgt cagacccgtg gcaacaatct tggataatcg agggatttga
     2701 atgggcctat cgtcttgcaa gctacccacc atagccaatc gtctttgata tcgatcatct
     2761 cgtccgacat gcgtgaggtt gatcgggtta tcgcccaacg tctggattct ggcgtgccac
     2821 tagtggggca ggtgtcgcgc tatattattt ctgctggcgg caagcgactg cgcccggtct
     2881 tgctgctgct gatttgtggt gccttgggct tcaagggtgt tcagcgcttt aacctcgccg
     2941 ccgtggtcga attcattcac accgctaccc tgttgcatga cgatgtggtg gacgattcct
     3001 cgctgcgacg tggcaacgcc acggccaatg agacttttgg caacccggcc agtgttttgg
     3061 tgggggactt cttgtactcg cgcgcctttc agatgatggt tgatgcagac aatatgcgca
     3121 tcatgcaggt gctggcggat gccactaaca tcattgctga aggcgaagtc atgcagttga
     3181 tgaacatgca caacgcggcc ctggatgaag ttggctacct ccaggtgatt cgctccaaga
     3241 cagcaaagct gtttgaagcc agtgcccgag tgggtgccat actggccggc gcatcacacg
     3301 agattgaggc ctgctgcgcg gattatggcc aagccatggg aaccgccttt caggtgattg
     3361 atgatgtgct ggactacacc ggggatgtgt cggtcatggg caagaacctg ggcgacgacc
     3421 tgcgcgaagg caaaaccacc ttgccgctga tcgctgccat gcagcgcggc gacgcggcac
     3481 agcgtgagct gatcaaggtg gccatcgaaa ccggcgatgt caaaatgatc aagcaggtgg
     3541 tggctatagt gcagtcaagt ggtgccatcg atgctgccag gcgagctgca atgctggagg
     3601 ccgagcgtgc cgccgccgcc gccagtcagc taccagaagg accccacaca gcctgtttgc
     3661 tacaattggc aactcaactg cttgagcgtc aatcgtgatg gtcaggcaag tgttttaatc
     3721 ggaatgtagc gcagcctggt agcgcacttc gttcgggacg aaggggtcgg agg
//
LOCUS       DAOSLL010000493         3770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_21371_length_3770_cov_4.381157, whole genome shotgun sequence.
ACCESSION   DAOSLL010000493 DAOSLL010000000
VERSION     DAOSLL010000493.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21371_length_3770_cov_4.381157"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PLB25_17590"
     CDS             complement(<1..1088)
                     /locus_tag="PLB25_17590"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HOE43422.1"
                     /translation="MSIARFLTGITTSGTPHLGNYVGSIRPAVRASLVQGVESFYFLA
                     DYHALIKVDEPTRIQRSTLEIAASWLAAGLDPARVVFYRQSDIPEIPELTWFLTCVLG
                     KGVLNRAHAYKAAVDKNTAASADPDTDVTAGLFMYPVLMGADILMFNAHKVPVGRDQV
                     QHIEMARDMAHSFNHRYGDHFVPPEAVIDDNVATLPGLDGRKMSKSYNNTIALFASRE
                     QLEKSIAAIVTDSKAPGEPKSTDGSALFQLYQAFASTEETEALSQAFADGIAWGQAKQ
                     IVFERIDHEIAPMRASYQALINDPAKIEAILNAGAIKARAIARPFMTRLRQAVGLRKL
                     DQQSDASSKAARLALPVFKQYREADGQF"
     gene            complement(1133..1801)
                     /locus_tag="PLB25_17595"
     CDS             complement(1133..1801)
                     /locus_tag="PLB25_17595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011796751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOE43423.1"
                     /translation="MDFANLIQTVTIYAIPVLLSITVHEAAHGYAARYFGDNTAYALG
                     RVTLNPFKHIDPVGTILMPLLLYVATSGAFLFGYAKPVPVRFGNLRNPKRDMVWVALA
                     GPGVNFIQALLWGVLLYLLQGAGVTEPFFVEMAQGGILVNVVMFVFNLFPLPPLDGGR
                     ILVGLLPYRQAMLVSRIEPWGFFIVMALVIAGVVSALWMRPLMGLSFKLINLLLSPLA
                     MLVT"
     gene            complement(1814..2437)
                     /locus_tag="PLB25_17600"
     CDS             complement(1814..2437)
                     /locus_tag="PLB25_17600"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HOE43424.1"
                     /translation="MPLIFAIHPDNPQPRLLKQATALLDKGEVLAVPTDSSYALVCHL
                     DDKAAADKLRRIRGIDDKHHLTLLCRDLSELSTYARVDNQQFRRLKAATPGAYTFILE
                     ASKEVPRRLSHPSRKTIGLRVPDHKVLQDLLALHNTPLLATTLIPPGDTEPLNDAQEI
                     RERYRGRLAAVIDSGACPREPTTVVDLTNSDPVVIRQGRGSLATLGL"
     gene            complement(2441..3325)
                     /locus_tag="PLB25_17605"
     CDS             complement(2441..3325)
                     /locus_tag="PLB25_17605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HOE43425.1"
                     /translation="MTFWRSRQGGGILLIVLATLAFALLDTATKHATQLAPVLMLLWF
                     RYLFQAVATFALRFPVQKRRLFDTPNPRFQALRGGLLLATSFCSFFGLQYLPVGEFTA
                     IVMLSPMVATALAALVLKNHVAPLRWMLMLVGLGGVLLVVRPGGQVFGWALLFPVVLV
                     GTYAWFQVLTSRLSADENPYTTHFYTGLVGTLVMCPVALLSWKTQALLDYWPWFLVLG
                     FLGTFGHLMLIRAFNRASAVVLAPYLYAQIAFATLAGWLAFSHVPDAYAWAGIAVIAS
                     SGVGNAVLSAREAQLKRQ"
     gene            complement(3343..>3770)
                     /locus_tag="PLB25_17610"
     CDS             complement(3343..>3770)
                     /locus_tag="PLB25_17610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase"
                     /protein_id="HOE43426.1"
                     /translation="CKDTYEVFRKYLTEGKPGYVEHRWAALKDAVRWISQAGGVAVIA
                     HPARYKFTANEEFALFTEFKAHGGQAVEVVTGSHSAAECVAYANTALEFGLAASRGSD
                     FHSPDESRTDLGALPGLPARLTPVWDLLSHRIQRAPGKA"
BASE COUNT          846 a         1120 c         1125 g          679 t
ORIGIN      
        1 aaaaattgac cgtcggcttc tcggtattgc ttgaagacgg gcagcgccag ccgggcggct
       61 ttgctgctgg catcactttg ctggtcaagc ttgcgcaagc cgacggcctg gcgcagccgc
      121 gtcatgaagg gcctggcaat ggcgcgcgcc ttgatcgcgc cagcattcag gatagcctcg
      181 attttggccg ggtcgttgat cagcgcctgg tagcttgccc gcatcggggc aatttcatgg
      241 tcgattcgct caaacacgat ttgcttggct tgtccccagg caatcccgtc ggcaaacgcc
      301 tggctcaacg cttcggtttc ttcggtgctg gcaaaagcct gatagagctg aaacagcgcc
      361 gagccatcgg tgcttttggg ctcgccaggt gctttggagt cggtcacgat agccgcaatc
      421 gacttttcca gttgttcgcg cgatgcaaac agcgcaatcg tattgttgta actcttgctc
      481 atcttgcgac cgtccaggcc cggcagggtt gcgacgttgt cgtcaatcac cgcttcaggt
      541 ggcacaaagt gatcgccgta gcggtggttg aagctgtgcg ccatgtcgcg tgccatttcg
      601 atgtgctgca cctggtcgcg cccaaccggc accttgtggg cattgaacat caaaatgtcg
      661 gcccccatca gcaccgggta catgaacagc ccagccgtca catcggtatc cgggtcggcg
      721 cttgcggcgg tgttcttgtc cactgcggcc ttgtaggcat gcgcccggtt aagcacgccc
      781 ttgccgagca cgcaggtcaa aaaccaggtg agttcgggaa tttcggggat atcagactga
      841 cggtaaaaaa ccacccgcgc cgggtccagt cctgcggcca gccaactggc ggctatctcc
      901 agtgtcgaac gctgaatgcg ggtcggctca tcaaccttga tcagcgcatg gtaatcggcc
      961 agaaagtaaa aactctcgac cccttgcacc aggctggcac gcactgctgg ccggatcgag
     1021 cccacatagt tgcccagatg cggggtgccg gaggtggtaa tgccggtcag aaaacgcgcg
     1081 atactcatga aaaaaacaca gtctcaaaag aacaaaaaaa ggggtcgcca aatcaagtca
     1141 ccagcatggc cagaggtgac agcagcaggt tgatcagctt gaagctcaat cccatcagcg
     1201 gtcgcatcca caaagcgctg acaacaccgg caatcaccag cgccatgacg atgaaaaatc
     1261 cccagggctc gatgcgtgac accagcattg cctgccggta gggcagcaag ccgaccagaa
     1321 tgcggccacc gtcaagcggt ggcaacggaa acaggttaaa gacaaacatg accacgttga
     1381 ccagaatgcc accttgcgcc atctcgacaa aaaatggctc ggtgacacca gcgccttgca
     1441 gcaggtaaag cagcacaccc cacagcagtg cctgaatgaa attgacccca gggccggcca
     1501 aagccaccca caccatgtca cgcttggggt tgcgcagatt gccaaaacgc accggcaccg
     1561 gcttggcata accaaacaaa aacgcgccag aagtggccac gtacagcaac aaaggcatca
     1621 ggatggtgcc gaccgggtca atatgcttga acggattgag cgtgacgcgc ccaagggcgt
     1681 aagccgtgtt gtcgccaaag tagcgtgccg cgtaaccgtg cgcagcctcg tgcacggtga
     1741 tcgacagcag caccgggatg gcgtagatag tgacagtttg aatcaggttg gcaaagtcca
     1801 tggcaggatt ctcctaaagg cccagcgttg ccagcgaccc gcggccttgg cggataacaa
     1861 ccggatcgct gttggtcaga tcaaccaccg tggtgggctc gcgcgggcag gcaccagagt
     1921 cgatgaccgc agcgagtcgg ccacgatagc gttcgcgaat ctcctgtgca tcgttaagcg
     1981 gctcggtgtc tccgggggga atcagggtgg tggccagtag cggtgtgttg tgcagtgcca
     2041 gcagatcctg caacaccttg tggtcgggca cgcgcaggcc aatggttttg cgtgacgggt
     2101 ggctcaaccg acgtggcact tccttgctgg cctccagaat gaaggtatag gcccctggcg
     2161 tggcggcttt caggcggcga aactgctggt tgtcgacgcg tgcataggtt gacagctcgg
     2221 acaagtcccg gcacagcagg gtcagatgat gtttgtcatc aatcccacgg atgcgccgca
     2281 gcttgtcagc ggctgccttg tcatcgagat ggcacaccag cgcgtagctg gagtcagtgg
     2341 gcacggccag cacctcgccc ttgtcaagca atgcggtggc ttgcttgagc aggcgtggtt
     2401 gcgggttgtc ggggtggatg gcaaaaatca gtggcatggc ttattgtcgc ttgagctgcg
     2461 cttcacgcgc agacagcact gcattgccga cgccactgct ggcgatcacc gcaattccgg
     2521 cccaggcata ggcgtccggc acatggctga aagccagcca gccagccagc gtggcaaagg
     2581 cgatttgtgc gtaaagatag ggggccagca ccacggcact ggcccggttg aaggcccgaa
     2641 tcagcatcag atggccaaaa gtgcccaaaa accccagcac caaaaaccac ggccagtagt
     2701 ccagcagggc ctgggttttc cagctcagca aggcgaccgg acacatcacc agggtgccga
     2761 ccagtccggt gtaaaagtgc gtcgtgtagg ggttttcatc agcactgagg cgactggtca
     2821 gcacctgaaa ccaggcataa gtgcccacca gcaccaccgg aaacaacagc gcccagccaa
     2881 acacttgccc acccgggcgc accaccagca gcaccccacc gaggcccacc agcatcagca
     2941 tccagcgcag cggtgccaca tggtttttca gcaccagcgc cgccagcgcc gtggccacca
     3001 tgggtgacag catgacgatg gcagtgaact cgccaaccgg aaggtactgc aggccgaaaa
     3061 acgagcagaa actggtggcc agcagcaagc cgccacgcag cgcctgaaat cttgggtttg
     3121 gggtgtcaaa aaggcgccgc ttttgcaccg gaaaccgcaa tgcaaaagtc gccacggcct
     3181 gaaacaggta acggaaccac agcagcatca agaccggtgc cagttgggtt gcgtgtttgg
     3241 tggccgtatc aagcaaggca aaggccagcg tggccagcac aatcagcaag atgccgccgc
     3301 cctgacggct gcgccagaat gtcattggcg cgtggctgcc agttatgcct tgcctggcgc
     3361 acgttgaatg cggtggctca acaaatccca taccggggta agcctggcgg gcaggccggg
     3421 cagcgcaccc aggtcggtgc gactttcgtc ggggctatgg aagtcgctac cccttgaggc
     3481 ggccaggcca aattcgagtg cggtgttggc ataggcgaca cattcagccg ctgaatggct
     3541 gcccgtaacc acctctacag cctgaccacc atgcgccttg aactcggtga acagggcaaa
     3601 ctcctcattg gcggtgaact tgtagcgagc cgggtgcgct atcacggcca cgccacctgc
     3661 ttgcgaaatc caacgcaccg cgtccttcag cgcagcccaa cggtgctcga cgtaaccggg
     3721 tttgccttca gtcaggtatt tgcgaaacac ttcataagtg tctttgcaga
//
LOCUS       DAOSLL010000494         3764 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_21419_length_3764_cov_3.981666, whole genome shotgun sequence.
ACCESSION   DAOSLL010000494 DAOSLL010000000
VERSION     DAOSLL010000494.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3764)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3764
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21419_length_3764_cov_3.981666"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /gene="ispH"
                     /locus_tag="PLB25_17615"
                     /gene_synonym="lytB"
     CDS             <1..857
                     /gene="ispH"
                     /locus_tag="PLB25_17615"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HOE43427.1"
                     /translation="VRHEIVHNTYVVNDLKAKGAIFIEALDAVPAGATLVFSAHGVSR
                     AVEQEATDRGFRIFDATCPLVSKVHVEVAKLAKEGFEFVMIGHKGHPEVEGTMGQLDG
                     GIHLVEDVADVARLAPGQTDKLALVTQTTLSVDDAAEIAAAIRRRFPKVREAKQQDIC
                     YATQNRQDAVKLMSPQVDLVIVVGSPSSSNSNRLAELARKLGTPGYMVDSSEDLQPQW
                     FAGCTRVGLTAGASAPEILVTQVIERIKAFGAVSVQTMAGLPETVKFPLPKGLRTDLN
                     HNESQDAT"
     gene            917..2245
                     /gene="serS"
                     /locus_tag="PLB25_17620"
     CDS             917..2245
                     /gene="serS"
                     /locus_tag="PLB25_17620"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HOE43428.1"
                     /translation="MLDITLLRKDLGSVIARLESRKSPQMFLDVQAFTALETERKTIQ
                     MRTEALQAQRNSASKQIGQLKAKGPAGAAQADAVMAQVAGIKDELDASAKRLEQIQLE
                     LQTLLLAVPNLPHDSVPVGADEHANVVVRTWGTPRVFDFAVQDHVDVGMPLGLDFEMG
                     VKLTGSRFTVMKGPVARLHRVLAQFMLDVQTQEHAYTECYTPYIVNAATLRGTGQLPK
                     FEGDLFAAKKGGQEGDEQLDNAALYLIPTAEVPLTNFVRDVVVAEAELPILLTGHTPC
                     FRSEAGSAGRDTRGLIRQHQFDKVEMVQIVHPDQSYAALEVMTGHAEAILQKLGLPYR
                     VMSLSTGDLGFGASKTYDLEVWLPAQNTYREISSVSNCEAFQARRLQARFKNAQGKNE
                     LVHTLNGSGLAVGRTLVAVLENYQNADGSVTVPEVLQPYLGGLKLLVPGL"
     gene            2292..3530
                     /locus_tag="PLB25_17625"
     CDS             2292..3530
                     /locus_tag="PLB25_17625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation pathway signal"
                     /protein_id="HOE43429.1"
                     /translation="MLDRLLAPVLIALAAIVTVGLSGCAYGPWQRAEAPPVVFVHGDD
                     AAATYWQDLVWRFESNGWPRSRLFALQQPHPQAPDDDAVAQPGRSSDADQLAFLKTEV
                     AQVLLKTGAKQVVLIGHGRGAYAIRNYIVKGGGDQTVSHAVLSWPDGLWAGANKKLSL
                     ADYESTALKGVKSLVIPRSEQRDGAFSVASFAAAYRFITGKPADNLTIWPQYDLVLDG
                     LVTGMGVASGDRASAGTHYFNNLPLSQAQLEIFVVQHDTGLRAGAAVYDKVVGPDGRW
                     GPFQAQQGARYEFVLRSPGYAVTHIYRSPFVRGTNFLHLKAERTADEDLPAFAIIHLE
                     RPRGYMDPTVNHVSFDGQFPPPGVAVTGGGRVNRSKITLNKLQYRAIAGEIHTDEVNR
                     VTGRTWPAKESHMVRLELAE"
BASE COUNT          756 a         1101 c         1162 g          745 t
ORIGIN      
        1 acgtgcgcca cgagatcgtg cacaacacct atgtggtgaa cgacctgaag gccaaaggcg
       61 ctatctttat tgaagcactt gacgcagtcc cggcgggggc taccctggtt ttttctgccc
      121 atggcgtgag ccgggcggta gagcaggagg cgactgaccg gggttttcgc atttttgacg
      181 ccacctgccc gctggtttcc aaagtgcatg tggaagtcgc caagctggcc aaagagggtt
      241 tcgagttcgt catgattggt cacaagggcc accctgaagt ggagggcacc atggggcagc
      301 ttgatggcgg cattcatctg gtcgaagacg tggccgatgt cgcgcgccta gcgccgggtc
      361 aaaccgacaa gctggcactg gtgactcaaa ccaccctgag cgttgacgat gccgccgaga
      421 tcgcggctgc catcaggcgg cgcttcccga aggtgcgtga agccaagcag caagacattt
      481 gttacgccac gcaaaaccgc caggatgccg tcaagctgat gagtccacag gttgatctgg
      541 tcattgtggt gggcagccca agcagttcca acagcaaccg gctggccgaa ctggcgcgca
      601 aactcggcac ccccggctac atggttgaca gttctgagga cctgcagccg cagtggtttg
      661 ccggttgcac ccgcgtcggc ttgaccgccg gtgcgtcagc gccagagatt ctggtgacac
      721 aggtgatcga gcgcatcaag gcatttggtg cggtgtcggt gcaaaccatg gcgggcttgc
      781 cagagacggt caagtttccg ctgcccaagg ggctgcgcac cgacctgaac cacaacgaat
      841 cgcaagatgc tacataacat atagcttcta gcgcttgtca gacaagggcc agagcccaat
      901 ttcatatata aagatcatgc tcgatatcac actacttcgc aaagacctcg gcagtgtcat
      961 cgcccgactg gaaagccgca aaagcccgca gatgtttctg gacgtgcaag ccttcactgc
     1021 gctggagacc gagcgcaaaa ccattcagat gcgcaccgaa gcactgcaag cccaacgcaa
     1081 cagtgccagc aagcaaattg gccagctcaa ggccaaaggc ccagccggtg cggcgcaagc
     1141 ggatgcggtg atggcgcagg tggcgggtat caaggacgag cttgacgcgt ctgccaaacg
     1201 gcttgagcag attcagttgg agttgcaaac ccttttgctg gcggtgccca acctgccgca
     1261 cgacagcgtg ccggtcggtg ccgacgagca tgccaatgtg gtggtgcgca cctggggtac
     1321 acccagggtg tttgattttg ccgtgcagga ccatgtcgat gtcggcatgc cgctcggcct
     1381 tgattttgag atgggggtca agctgacggg ctcgcgcttt accgtgatga aagggccggt
     1441 ggcgcggctg caccgggtgt tggcgcagtt catgctcgat gtgcaaacgc aggaacatgc
     1501 ttacaccgag tgctacaccc cgtacattgt caacgccgcc acgctccggg gcaccggcca
     1561 gttgcccaag ttcgagggcg atttgtttgc tgccaaaaag ggcgggcaag agggcgatga
     1621 gcaactggac aacgctgcgc tgtacttgat tccgacagcc gaagtgccac tgaccaattt
     1681 tgtccgcgat gtggtggtgg ctgaggccga actgccgatt ctgttgaccg gccacacccc
     1741 atgtttccgc tcggaagccg gcagcgccgg gcgtgatacc cgcggcctga ttcgtcagca
     1801 ccagtttgac aaggtcgaaa tggtgcaaat cgttcacccg gaccagagtt acgcggcgct
     1861 cgaagtgatg accggccacg ccgaggccat tttgcaaaag ctcggcttgc cgtatcgcgt
     1921 gatgagcctg agcaccggtg acctgggctt tggtgccagc aaaacctatg accttgaagt
     1981 ctggttgcca gcacaaaaca cctaccgcga aatcagctcg gtcagcaact gcgaagcctt
     2041 tcaggcgcgc cgcctgcagg cccgcttcaa gaacgcgcag ggcaagaatg agctggtgca
     2101 cacgctcaat ggctccgggc tggctgttgg ccgaacgctg gtggcggtgc tggaaaatta
     2161 ccagaatgcc gacggttcgg tgacggtgcc cgaggtgttg cagccgtatc tgggcggcct
     2221 caagctgctg gtgccagggc tttgatgcgt tttgttttga ccgacgatca tcggttgttg
     2281 ctggagtttt tatgttagat cgccttctag cccccgtcct tattgcgcta gctgctattg
     2341 ttactgtagg gttgtcgggt tgtgcttacg gcccgtggca acgggcagag gcacccccgg
     2401 tggtttttgt ccatggcgat gatgctgcgg caacctattg gcaagacctg gtttggcgtt
     2461 ttgagtccaa tggctggccg cgctcgcgcc tgtttgcctt gcaacagccg cacccgcaag
     2521 ccccggacga cgacgcagtg gcgcagcccg gtcgcagctc ggatgccgat caactggcgt
     2581 tcttgaaaac cgaagtggcg caagtactgc tcaaaaccgg tgccaaacag gtggtgctga
     2641 tcggccatgg gcgcggtgct tacgcgattc gcaattacat cgttaagggc ggcggggacc
     2701 agaccgtcag ccacgcggtg ttgtcctggc ccgatgggct gtgggccggg gccaacaaga
     2761 aactgagcct ggcagactat gaaagcacgg cgctcaaggg tgtcaaaagc ctggtgattc
     2821 cccgctcgga acaacgtgac ggtgccttct cggtggcctc gtttgccgcc gcctaccgct
     2881 ttatcaccgg caagccagcg gacaacctca ccatttggcc gcagtatgac ctggtgctcg
     2941 acggcctggt caccggcatg ggcgtggcat ctggtgaccg cgccagtgct ggcacgcact
     3001 atttcaacaa cctgccgctg tcccaggcgc agctcgaaat ttttgtcgtt cagcacgaca
     3061 ccggcttgcg tgctggcgca gcggtgtacg acaaagtggt cggacctgac ggccgctggg
     3121 ggccgtttca ggcgcagcag ggcgcacgtt atgagtttgt gttgcgctcg cctggctatg
     3181 cggtgacgca catctatcgc agcccgtttg tgcgcggtac caatttcctg cacctgaagg
     3241 ccgagcgcac tgccgacgag gacttgccgg cctttgccat catccacctg gagcgcccgc
     3301 gcggctacat ggacccgacc gtcaaccatg tcagctttga tggccagttt ccgccaccag
     3361 gtgtcgcggt caccggtggt ggtcgggtca accgcagcaa gatcaccctg aacaaattgc
     3421 agtaccgggc catcgccggg gaaattcata ccgatgaggt caatcgggtc actggccgga
     3481 cctggccggc caaagagagc cacatggtgc ggctggaact ggccgagtag cccgacccct
     3541 ggacccgccg gtcgtacttg atggatcgaa cagcgagaag cgcccccagc gaggccagtt
     3601 tatgccggac ttgcaggccc attgcccggt tgtggcccga tcaacaggtg caaaatgggc
     3661 cgccatcccg cagcccatgc gccatcagtc tgaccggcta ttggtctcca acaagtcaag
     3721 tctttgcata tcatgcgaag aattcaaaca ttccaagtca ttcg
//
LOCUS       DAOSLL010000495         3757 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_21468_length_3757_cov_5.201243, whole genome shotgun sequence.
ACCESSION   DAOSLL010000495 DAOSLL010000000
VERSION     DAOSLL010000495.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21468_length_3757_cov_5.201243"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3108)
                     /locus_tag="PLB25_17630"
     CDS             complement(<1..3108)
                     /locus_tag="PLB25_17630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE43430.1"
                     /translation="MNFFKPIAAWLGRLSVSRKLTLIYLLDLTAVIYISGILIHEKYL
                     AIDFARKEIVGIAYSDSVRELLMAAIRPAPLAPAEAQAVLQQFGLQRARHDATLNAQA
                     DSAALVSSWQFLQGGGNPSADALQPVIQGARNLITTVGNQSNLILDPDLDSYYSMSLV
                     VLRFPELVEVLNDVIRLVDHQPRAGRGQVDLQSTRLLILAGRLDAIHQGITADYKQAY
                     AAGSPAFRSQLLDQQIALNRQLLQLLSMVQTFSEKNILPTDVQQLALLQGQTIDALAQ
                     AWQATSGALNALLQQRVDSLFAKMWLHLGTALALLLAILSLVYTVARLIAQPIKQLAG
                     VANTVRQTGDYNLRAHWASQDEIGHLVTTFNSMLAQLDRDRDARQELAASTRAAQAQL
                     ELLESIPIAMVVTSVPDHQVLHANEPAAPWLAGRSTDPWRHGLEPGVRVRFFQRLSDH
                     DRVDEFEVRWLGGAEPSWAVLSARRLSFQGHDALLTTFTPINVLKLMEQRLELWAKVF
                     EASSEGIIILDAGQKVLSVNTAYCKATGYDFYEVIGEHLHMLLGNTAAESDEELAGEI
                     SRTIAQRHEWQGEVLLRKRSGQTYPAWLMISAVRESNRKGTVSHYIGISIDITDRKRT
                     EARVQFLAKHDALTELPNRALCVETLQAALKQAGNSGEYLAVLFIDLDRFKVINDTLG
                     HHIGDGLLRSVAQRLQQAVRAGDLVSRLGGDEFVVILRDIKTGEQARWQVEQRLIPLV
                     RQTHSVQGHALNVSCSVGIAVYPTDGLDLDELMRRADAAMYEAKAGGRDAARVFDSSI
                     DQVIRERQELEQHLRLALERGEFSLNYQPRLDARSGVVLGVEALIRWHSTELGSIPPG
                     RFIPVAEECGLIHPIGRWVLDEACRQRASWQQQGLASLVMSINLSAAQLAEPDLVDLV
                     RQCIERHHCDPSGLELEITESHLMADPVAANEKLIAIKALGLQLSIDDFGTGYSSLAY
                     LKRFPIDKLKIDQSFVRDMLDDPADMAIVRAIVVLGHTLGLTVVAEGVEQKEQAGQLR
                     QLGCDE"
     gene            complement(3602..>3757)
                     /locus_tag="PLB25_17635"
     CDS             complement(3602..>3757)
                     /locus_tag="PLB25_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HOE43431.1"
                     /translation="RRAGKLRAELQYGRIDEILATGLHAYLTQFLDRINDLGANISRD
                     FLVPMPS"
BASE COUNT          695 a         1195 c         1139 g          728 t
ORIGIN      
        1 ttcgtcacaa cccaactgcc tgagttgccc ggcctgttct ttttgctcca cgccctcggc
       61 caccaccgtc aggcccagcg tatggccgag caccacaatg gcccggacaa tcgccatgtc
      121 ggccgggtcg tctagcatgt cgcgcacaaa agactggtca atcttcagct tgtcaatcgg
      181 aaagcgcttc aggtaggcca ggctggaata gccggtgcca aaatcgtcaa tcgagagttg
      241 cagcccgagc gccttgatgg ctatcagctt ttcgttggca gccaccggat cggccatcag
      301 gtgcgactcg gtgatttcaa gctccagccc actcgggtcg cagtgatgcc gctcgatgca
      361 ttggcgcacc aggtccacca ggtcgggttc ggccaactgg gccgccgaca ggttgatcga
      421 catcaccagg gaggccagcc cctgctgctg ccaactggcg cgctggcggc aggcctcatc
      481 gagcacccag cgcccgatcg ggtggatcag gccgcactct tcggccaccg gaataaagcg
      541 cccaggcggg atcgagccga gttcggtact gtgccagcgg atcagcgctt ccaccccgag
      601 cacgacaccg ctgcgggcat ccagccgggg ctggtaattc aggctgaatt cgccgcgttc
      661 gagtgccagc cgcaggtgct gctcaagctc ctggcgttcg cggatgacct ggtcaatcga
      721 cgaatcaaat acccgcgccg catcgcgacc gccggccttg gcttcgtaca tcgcggcatc
      781 ggcgcggcgc atcagttcat caaggtccag cccgtcggtc ggatagaccg caatgccgac
      841 gctgcacgac acattgagcg cgtgcccctg cacgctgtgg gtctggcgca ccagcgggat
      901 caggcgctgc tcgacctgcc agcgcgcctg ctcgccggtc ttgatgtcgc gcaaaatcac
      961 cacgaattcg tcgccgccga gccgactcac cagatcgcca gcgcgcaccg cctgctgcag
     1021 gcgctgcgcc accgagcgca acaagccgtc gccgatgtgg tgccccaggg tgtcgttgat
     1081 gaccttgaag cggtccagat caatgaacag cacagccagg tattcaccgc tgttgccagc
     1141 ctgtttgagc gcggcctgca gcgtttcgac acacaaggcg cggttcggca gctcggtcag
     1201 cgcgtcgtgt ttggccagaa actgcacccg cgcctcggtg cgtttgcggt cggtgatgtc
     1261 gatcgaaatg ccaatgtaat gcgataccgt gccttttcga ttggattcgc gcaccgccga
     1321 gatcatcaac caggccgggt aagtctggcc gctgcgcttg cgcaacagca cttcgccctg
     1381 ccactcgtgg cgctgcgcga tggtgcgcga gatctcgcca gccagttcct cgtctgattc
     1441 ggcggcggtg ttgcccagca gcatgtgcag gtgttcgccg atcacctcat aaaagtcata
     1501 gccggtggcc ttgcaatagg ccgtgttcac gctcaacacc ttttgccccg catccagaat
     1561 gatgatgccc tcggacgaag cctcgaacac cttggcccac agttccagcc gttgctccat
     1621 cagcttgagc acattgatcg gagtgaacgt ggtcagcaag gcatcgtggc cctgaaagct
     1681 caggcggcgc gccgacagca cggcccatga cggctcggca ccgccgagcc agcgcacctc
     1741 gaattcatcg acccggtcat ggtctgacag gcgctggaaa aaccgcaccc gcacgcccgg
     1801 ttccaggccg tgtcgccagg ggtcggtgct gcgcccggcc agccagggtg cggccggttc
     1861 gttggcgtgc agcacctgat ggtcgggcac tgaagtcacc accatggcaa tcggaatcga
     1921 ctccagcagt tccagttggg cctgcgcggc gcgcgtgctg gcggccagtt cctggcgcgc
     1981 atcgcggtcg cggtcaagct gcgccagcat gctgttgaaa gtggtgacca ggtgaccgat
     2041 ctcgtcctgg ctggcccagt gggcgcgcag gttgtagtca ccggtctggc gcaccgtgtt
     2101 ggcaacaccg gcaagctgct tgatcggctg ggcaatcaat cgggccaccg tatagaccag
     2161 gctcagaatg gccagcagca gtgccagggc ggtgcccaga tgcagccaca ttttcgcaaa
     2221 caggctgtca acgcgctgtt gcagcagcgc attcaaggca cctgatgtcg cttgccaggc
     2281 ctgcgcaagt gcatcaatgg tctgtccctg gagcagcgcc aactgctgca catcagtcgg
     2341 caggatgttc ttttctgaaa aagtctgcac cattgacagc agttgcaaca actggcgatt
     2401 cagtgcaatt tgctggtcaa gcagttggct tctgaaggcc ggactgccag ccgcataggc
     2461 ctgcttgtaa tcggccgtta tgccctggtg aatggcgtcg agccgacccg ccagaatcag
     2521 cagacgagtg ctttgcaaat ccacctggcc gcggcccgcg cgcggctggt ggtcaaccag
     2581 gcgaatcaca tcgttgagga cctcgaccag ttcgggaaac cgcagcacca ccagcgacat
     2641 gctgtagtaa ctgtccagat cagggtccag aatcaggttc gactggttgc ccacggtggt
     2701 gatcaaattg cgcgcaccct gaatcactgg ctgcaatgca tcggccgacg ggttgccgcc
     2761 gccttgcaag aactgccagc ttgacaccag cgccgcgctg tcggcctgcg cattcagggt
     2821 ggcatcgtgg cgtgcccgct gcaaaccaaa ctgctgcagc accgcctgcg cctcggccgg
     2881 tgcaagcggt gctggccgga tcgccgccat cagcagctca cgcacgctgt cactgtaagc
     2941 aatgccgaca atttccttgc gggcaaagtc gattgccagg tatttttcgt gaatcaggat
     3001 gcccgatatg tagatgacgg cggtcagatc gagcaggtaa atcagcgtca gtttgcggct
     3061 gacgctaagg cgtcccagcc aggccgcaat cggtttgaag aagttcatga tgggccaggc
     3121 tggcggtcac gccagtaccg ctcgggcatg aaaacccgag cgcatcatga tgctggattc
     3181 ctcggttgac cgtttgcttt catcagtaag tccttgtgat tatttgccaa tttgcttcga
     3241 tgaggttcac ctgtcgggtg actgcgcaac gcacctgatt gagccgctgg caacgcacct
     3301 gggggtgttg tcccatttct aaatcatccc tgtcgttgct atctcgcctt gccttgtgtt
     3361 tctgctggca gcgcacctgg atatgttatt cctcctggcg ggcaatgctg tgcgcggtca
     3421 tcacccctcg cagcgtgctg cagcatggcg ggcgcagcca cgccacaagg ctggcctgct
     3481 tcaagaagtc caaacatgcg caggcacatt tggcgctgcg gctttcgccc tgcccaggcg
     3541 caccgccggt agcccaccgg ggcgggtcca acccggccat ccaggacgat gctggcgcga
     3601 ctcagcttgg catcggcacc aggaagtcgc ggctgatgtt ggcaccgagg tcgttgatgc
     3661 ggtcaagaaa ctgcgtcagg taagcgtgca gcccggtggc caggatttca tcaatgcggc
     3721 cgtactggag ttcggcgcgc agtttgccag cccggcg
//
LOCUS       DAOSLL010000496         3749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_21530_length_3749_cov_4.626151, whole genome shotgun sequence.
ACCESSION   DAOSLL010000496 DAOSLL010000000
VERSION     DAOSLL010000496.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21530_length_3749_cov_4.626151"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(932..1906)
                     /locus_tag="PLB25_17640"
     CDS             complement(932..1906)
                     /locus_tag="PLB25_17640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008115527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOE43432.1"
                     /translation="MKKSLVALAVLAASGAAMAQSSVTLYGVVDAGLAWEDNGATTTT
                     RMDSSNLNGSRWGLKGTEDLGNGLKAIFVIESGFATDTGAQASATSLFNRQSYVGLSG
                     GFGTVKFGRQMNPVYSNSGTFDPFGDALAGDSARLLNYQGSRTDNAITYAYAASGFRG
                     EFQYALGEVAGNSTASRTYAGFVGYKNGPIDVVLTHQNTRNATDTDSTKVTLLGGNYD
                     FGVAKAFLTFDVEKGTGTTDLRHMIVGATVPIGAGSLMVSFIQRQDQAVDNKDAEQLA
                     IGYTYNLSKRTALYTSYSRLNNDDKSSVKVVKAGETNSLFNLGVRHFF"
     gene            complement(2163..3293)
                     /locus_tag="PLB25_17645"
     CDS             complement(2163..3293)
                     /locus_tag="PLB25_17645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOE43433.1"
                     /translation="MNQKRRSPSECALKCGFRESSWLNQTRDVSSACSKRLYHKVLNV
                     LLIFWSFEMKKSLIALAVLAASGASFAQSSVTAYGIADIWLGTVKAGGVRTTKLDSGG
                     VDTSRWGLKGTEDLGGGLKANFKLEQGFNLDSGSATSGQAFSRYAYVGFSGGFGEVKL
                     GKTGTAYDDVQGASDAVFDSALAPMNHVFASTGYIWNPANTIYYATPNMSGFSGAFSY
                     SLDEKVAATPKISSVNVAYAGGPLAVQFGYQKEDKTNVANDTSFLRLGGSFNFGVATA
                     KATYGEVKNVDNVADAKAKDWQLGVDVPVGPALTLSASYAQSDENARAGDTTGKGWGF
                     AGAYTLSKRTYLYGGFTSNKYTTPRLADSKLEIFALGVNHKF"
     gene            3536..>3749
                     /locus_tag="PLB25_17650"
     CDS             3536..>3749
                     /locus_tag="PLB25_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017425360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOE43434.1"
                     /translation="MTHSTLKSARPGAIQCIAIATALCCAGAAHAQMSPGVTLYGLAD
                     AGITSVSGLKGGTVTQLASGIMEGSRW"
BASE COUNT          890 a         1052 c          955 g          852 t
ORIGIN      
        1 gcgcccaaat ccgccggtgc aaatagggga agttatttct gaccacatcc taagggcgca
       61 agcgccagag tcgccctgcg catctgttgc ttggtgttcg gtcaacctgt gtcggtccag
      121 tcattccgtt actaaaccat ccatgccgtt gttacttcgc cgtgccttgc gtttgcaatg
      181 tctgcggcac gtaccttgag ccggtcggac tgatgtcgca tcggctgcga acagaccgca
      241 gaggcccatg ctgcaccggc catgttgact ccaagctggg ttgtgggccg gtaaatcggg
      301 cacccctggc tgggggtgct tctcgcactc gatccgaaag gccgacaggc tcccagccag
      361 aagatcgctc gctcgtgcgg ggtttgacca gcgggcagcg ctttgcactc gatcacccac
      421 cctcacccca aaaccaccta ttacctgccc tcactttcat ccaaggccaa ggctttccct
      481 tggttcttct tgatgccaga cgtgcttgag acacccccca tggcaggcgc tggacgactc
      541 aatgcaaatc aattttcaga gcacgctgcg cagtatctac gggggcttga gtgctattta
      601 gagcatcaac gaggggcaat ctgaagcaat cgccatgaac aaggtgctac tggcgatggg
      661 ctacgacgat gggtttcggg cgaattgatg tctgtcagag gtttgtgttt gtcacggctc
      721 caagttcagc atgcggtggt caagtttgcc gcggtgccca gacaagcgct ggcggtactg
      781 ccgccagacc tgcgaccagc ccatgtgcat ccagacatct tgcgaaaaat ctgggcccaa
      841 tgcacgcgat gcttcaaacg caaaaaaccc cgccagggtg ccccggcggg gtttgaacga
      901 tcagcgtcaa atgacttgag tctgcgctgg actagaagaa gtggcgcaca cccagattga
      961 acaacgagtt tgtctcgcca gctttcacca ctttgactga agacttgtca tcattgttca
     1021 aacggctgta actggtgtac aacgcagtgc gcttggacaa attgtaggtg tagccaatag
     1081 ccaattgctc tgcgtctttg ttatcgacgg cttggtcttg acgctggatg aaagacacca
     1141 tgagtgagcc agcaccgatg ggaacagttg caccaacaat catgtgacgc aggtctgttg
     1201 taccagtgcc tttttcaacg tcaaaagtca ggaacgcctt ggccacgcca aagtcgtaat
     1261 tgccgcccag caaggtcact ttggtgctgt cagtgtcggt tgcgttacgc gtgttttgat
     1321 gggtcaacac aacgtcgatc gggccgtttt tgtaaccgac aaagccggcg tacgtgcgac
     1381 tggcggtgct gttaccagca acttcaccca atgcgtactg gaattcacca cggaagccgc
     1441 ttgcagcgta cgcataagtg atcgcattgt cagtgcggct accttggtag ttcagcaggc
     1501 gagcggagtc acccgcaagc gcatcaccga acggatcaaa agtgccggag tttgaataca
     1561 ccggattcat ttggcgacca aatttgacgg taccaaaacc accgctcaag ccaacataag
     1621 actggcgatt aaaaagagat gtggcgcttg cttgagcgcc agtgtcagtg gcaaaaccgc
     1681 tttcaatgac gaaaatggcc ttcagaccat taccgagatc ttcagtaccc ttcaggcccc
     1741 aacgactgcc attcaagttg cttgaatcca tgcgggttgt agtggttgca ccattgtctt
     1801 cccatgccag gccagcatcg accaccccgt acaaggtcac agaagattgc gccatggcgg
     1861 caccggatgc ggccagcaca gccagcgcga cgagcgattt tttcattgag ttactccaaa
     1921 gttgatttcg gtgaagcgct atatcgcact tcgttacact tcttcaccag aagctaaagc
     1981 gattaaaaca gacccggaat gcctcgcaaa gtgatttcgc gacttatgag atgtctctgc
     2041 gcaccaatgt ggggtgccta caacgtttgg cggtacgcgc cagatcaaac ctctgggctc
     2101 tccaataaaa aagccgccct gtgggcggct tctagaaaca aacctgatca ggtctgcctt
     2161 gctcagaact tgtgattcac gcccaaagcg aagatctcga gtttgctatc ggccaggcgc
     2221 ggcgttgtgt acttgttcga ggtgaagcca ccgtacaagt aggtgcgctt ggacagggtg
     2281 taggcaccgg caaagcccca gcccttgcca gttgtgtcac cagccctggc attttcgtca
     2341 gactgtgcgt agcttgcaga caatgtcaat gcaggtccga ccgggacatc cacaccgagt
     2401 tgccaatcct tggccttggc atcagccaca ttgtcgacat tcttgacttc gccgtaagtt
     2461 gctttagcag tcgctacgcc gaagttaaac gaaccgccca aacgcaggaa agaagtgtca
     2521 ttggcgacat tggtcttgtc ttctttttgg taaccaaact gcacagccag agggccacca
     2581 gcatatgcaa cgttcacgct cgaaatttta ggtgttgcag caactttttc atccagactg
     2641 tagctgaatg cgccgctgaa accgctcatg ttcggggttg catagtaaat tgtgttggca
     2701 ggattccaaa tataccccgt gctcgcgaaa acatggttca ttggagccaa cgcagagtca
     2761 aacaccgcat cagacgcacc ttgcacgtca tcatatgcag tgcctgtttt gccaagcttg
     2821 acttcaccaa aaccgccgga aaaaccgacg taggcgtaac gagaaaaagc ctgcccacta
     2881 gtggcggaac cactgtccaa attgaaacct tgttccagtt tgaagttggc cttcaaacca
     2941 ccacccaggt cttcggtacc tttcaggccc cagcgcgaag tgtccacgcc gccgctgtcg
     3001 agcttggtgg ttctcacccc acctgctttg accgtaccca gccagatgtc agcaatgccg
     3061 taggcggtaa cagaagactg ggcaaacgaa gcacccgaag cagcaagcac tgccagagca
     3121 attagacttt ttttcatttc aaaactccaa aaaattaaca atacattcaa aaccttgtga
     3181 tacaagcgct tcgaacaggc ggatgaaaca tcacgggttt gatttaacca tgaagattcc
     3241 cgaaaaccgc atttgagagc acattccgat ggactgcgtc gcttttggtt caattttgtc
     3301 gctccaatgc aacaaactca ggtttcgctt caggtccaga aaaccatctg gctgccgtcg
     3361 atttatctaa ttccggcaag gggttagcgc gcggtgccgg aattgtcccg atgcaccatt
     3421 tcagggaaaa cccttgtagg gaatcacccc aaaaaagcgc accatcaaca gcattgaacg
     3481 cgtgcccatt gcgctggcga cttcaaacaa tttccaactt aacttctcaa tcatcatgac
     3541 tcattcaaca cttaagtcgg cccggcccgg tgccatccaa tgcatcgcta ttgccactgc
     3601 gctgtgctgt gcaggcgctg cccatgccca gatgtcgccg ggtgtcacct tgtacggttt
     3661 ggctgatgcg ggcattacct cggtcagcgg cctcaaaggt ggcaccgtga cccaactcgc
     3721 cagtggcatc atggaaggtt cacgctggg
//
LOCUS       DAOSLL010000497         3719 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_21757_length_3719_cov_4.631823, whole genome shotgun sequence.
ACCESSION   DAOSLL010000497 DAOSLL010000000
VERSION     DAOSLL010000497.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3719
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21757_length_3719_cov_4.631823"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..1311
                     /locus_tag="PLB25_17655"
     CDS             142..1311
                     /locus_tag="PLB25_17655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013803110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme enzyme"
                     /protein_id="HOE43435.1"
                     /translation="MKLSVTSALAGALAGVFGLSGCGGGQAPDTSAADPAASWAWTLP
                     NGFPVAAVPADNPMSAAKVDLGRFLFYDKRLSGNGTQACSGCHFQGKAFTDGLAVSRG
                     STGESTVRNAQALGNVAYSPTLTWANPSLVALERQMEVPLFGENPVEMGINDGNKAAV
                     LQRLADAALYPPKFAAAYPAEANPVTWGNVIKAIAAFERSLISGNSKYDQASVGKAVL
                     TAEEQRGKDLFFGDKAGCTQCHNGFNFNDQVADASTLVIEKPFHNIGLYNVGGTGAYP
                     EPNRGVMELSGLYQDMGAFRAPSLRNVAVTAPYMHDGSIATLEEVIDVIADGGRNVTT
                     GVNAGDGRLNPYKSKLVVALALSAQDKSDLIAFLKALTDHEFLGNPLQSDPFQSP"
     gene            1505..2140
                     /locus_tag="PLB25_17660"
     CDS             1505..2140
                     /locus_tag="PLB25_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43436.1"
                     /translation="MNTSASNPGKAFLAAREELPTTDQAQMWRVLSGALRIDSAVEGE
                     ASRFMRLALPGDVIGVEQWVGTADRLSFRALTEVCLTPVATKGTPVMQILMETVVVAH
                     QRCREVVSLRTGPVSRRVKCLLLMFADSVSPDATRSSECALPNLSDMADILDAAPETV
                     SRVFSSMRQLDFLQDRKPHKARFSSLTLRAQELIPGMTVSSALQRSQLAGV"
     gene            2144..>3719
                     /locus_tag="PLB25_17665"
     CDS             2144..>3719
                     /locus_tag="PLB25_17665"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HOE43437.1"
                     /translation="MSHTNCRFTTLATVLAALPFALAAQEARTLSPVTVSAAKIAPLG
                     ATETTDATLAPLRAASSDSARLLQNVPGLSLYGAGGVSSLPAIHGLADDRLRIQVDGM
                     DLIAACPNHMNSALSYIDPTRVGSILVFAGITPVSIGGDSIGGTIAVKSAAPAFADSP
                     EQVTGKGQLGTFYRSNGNASGLNLGATFIGQDVNVSYHGSRAESANYSAGSDFKSAGP
                     GAVGGPWLAGNEVASSRFTSENQDIALALRHDNHLLQLGLGQQHIPFEGFPNQRMDMT
                     GNDSTQINLRFNGRYAWGQLEARAFGQNTDHAMNMGPDRFSYGSLGMPMNSTAKTRGA
                     ALQAELLHSERDGFKAGIESYRYTLYDWWPQAGGVMGPATFWNVDYGTRDRVAIFGEW
                     EAQWNAQLLSQFGIRHESVKTDAGPVQGYNAQAIWASDAAAFNAQSRQRTDQNLDLTA
                     LARYTDNATQTFEAGLARKTRSPNLYQRYPWSTQPMATLMNNFVGDGNGYVGNLNLKP
                     EVAHTLSASGDWHDAGQ"
BASE COUNT          739 a         1142 c         1157 g          681 t
ORIGIN      
        1 cctctttaac gagctgcaaa tcagctttgg cagcggctcc actggcctgc cgatcaacgg
       61 cggcgcagcg cagaagatct tcaaggcggt tgcaaaataa tgtctgaaca ccgcctaatg
      121 tcggaacacc gccttttcag cttgaaactg tcggtgacca gcgcgctggc aggggcgctg
      181 gcaggtgtgt tcggcctgag cggctgtggc ggcggtcaag cgccggatac cagcgccgcc
      241 gacccggctg ccagttgggc ctggaccttg ccgaatggct ttccggtggc ggcggtgccc
      301 gccgacaacc cgatgtctgc cgccaaagtg gacctgggcc gctttctgtt ttatgacaag
      361 cgcctgtccg gcaatggcac ccaggcctgc tcgggctgcc atttccaggg caaggccttt
      421 accgatggct tggcggtgtc gcgcggctca accggcgaat ccacggttcg caatgcgcaa
      481 gccctgggca acgtggccta tagcccgaca ctgacctggg ccaacccgtc gctggtggcg
      541 ctggagcggc agatggaagt cccgctgttt ggcgagaacc cggtcgaaat gggcatcaac
      601 gatggcaaca aggcagccgt gttgcaacgc ctggcagatg ccgccttgta cccgccgaaa
      661 ttcgctgccg cgtatccggc cgaggccaat ccggtcacct ggggcaatgt gatcaaggcc
      721 atcgccgcct ttgagcgctc cctcatcagc ggcaacagca agtacgacca ggccagtgtt
      781 ggcaaggcgg tattgacagc cgaagagcaa cgtggcaaag acctgttttt tggtgacaag
      841 gctggctgca cccaatgcca caacggcttc aatttcaatg atcaggttgc agatgccagc
      901 accctggtta tcgaaaagcc gtttcacaac atcggtctgt acaacgtcgg tggcaccggc
      961 gcataccccg agcccaatcg gggtgtgatg gaactcagtg gtttgtacca ggacatgggt
     1021 gcttttcggg caccgagcct gcgcaatgtg gcagtgaccg cgccctacat gcacgacggc
     1081 agcatcgcga cgctcgaaga ggtgatcgac gtgattgccg atggtggccg caatgtcacc
     1141 accggtgtca acgcgggtga cggaaggctg aacccttaca agagcaagct ggtggttgcg
     1201 ctggcgctga gcgcacagga taagtcggac ctgatcgcct tcctcaaagc gctgaccgac
     1261 catgagtttc tcggcaatcc gttgcagtcc gacccgttcc agagcccatg aagacagctc
     1321 gccaaacgca aaggggctac cgacttgcag caccggcgag ctggaccgcc ctgatgcctg
     1381 ttgctcaaac ccttgttgcg cggacccggc aggggcctcg ctttgctact tttaatatag
     1441 ctgcttgcgc aacatacaca agggctggag gccaatttag tcaatgaaaa aggaaagggt
     1501 atccatgaac acctcagcat caaaccccgg caaagccttc ctggcggctc gtgaagaatt
     1561 gccgacgact gatcaggcac agatgtggcg cgtgctgtcg ggtgccttgc gcattgacag
     1621 cgcggtggaa ggcgaagcca gccgcttcat gcggctggca ctgcccggtg acgtgattgg
     1681 cgtcgagcaa tgggtcggca ctgctgaccg gctgagcttt cgggccttga ccgaggtctg
     1741 cctgacaccg gtggcaacca agggcacgcc ggtgatgcag attctgatgg aaaccgtggt
     1801 ggtggcgcac cagcgctgcc gcgaggtggt gagtctgcgc accggcccgg tgtcgcgccg
     1861 ggtcaaatgt ctgctgctga tgtttgccga cagcgtcagc cccgatgcca ccagaagttc
     1921 ggagtgtgcg ttgcccaacc tgagcgacat ggccgacatt ctggatgccg cccccgagac
     1981 cgtgtcgcgc gtgttcagca gcatgcgcca gcttgacttt ctgcaggacc gcaagccaca
     2041 caaggcccgc tttagcagcc tgacactgcg ggcgcaggag ttgattccgg gcatgacggt
     2101 cagctcggcc ctgcagcgca gccagttggc gggtgtttga gtcatgtcgc ataccaactg
     2161 tcgcttcacc accctggcca cagtgttggc agccctgccg tttgcgcttg ccgcgcaaga
     2221 ggccaggacg ctgagcccgg tgaccgtcag cgccgccaaa atcgcgcccc tgggtgccac
     2281 cgaaaccacc gatgccacac tggccccgct gcgcgccgcc agcagcgaca gcgcccgcct
     2341 gctgcaaaac gtgcctggct tgagcctgta tggtgctggc ggcgtgtcga gcctgcctgc
     2401 catccacggc ctggccgatg accggctgcg gattcaggtt gacggcatgg atttgatcgc
     2461 ggcctgcccg aaccacatga attcggcgct gtcctacatt gacccaacca gggtcggcag
     2521 catcttggtg tttgccggca tcacgccggt aagcatcggc ggtgacagca ttggtggcac
     2581 gattgccgtc aaatctgccg cgccggcgtt tgccgacagc cccgaacaag tcaccggcaa
     2641 agggcaattg gggacgtttt atcgcagcaa cggcaacgcc agcggactca acctgggggc
     2701 cacgtttatc gggcaagacg tgaatgtgtc gtaccacggc tccagggccg aatcggccaa
     2761 ctacagcgct ggcagcgact tcaagtctgc tggccccggc gctgtgggcg gaccctggct
     2821 ggctggcaac gaggtcgcat caagtcgctt tacctcagag aaccaggaca ttgcgctggc
     2881 gctgcgccac gacaaccatt tgctgcaact ggggcttggc caacagcata ttccgttcga
     2941 gggctttccc aaccaacgca tggacatgac cggcaacgac agtacgcaaa tcaacctgcg
     3001 ctttaacggc cgctatgcct ggggtcaact ggaggccagg gcttttggac aaaacaccga
     3061 tcacgccatg aacatggggc cggaccggtt ttcttacggc tcgctgggca tgccgatgaa
     3121 ttcaactgcc aaaacccgtg gtgccgcgct gcaggcggag ctgctgcatt cagagcgaga
     3181 tgggttcaag gcgggcatcg aatcctaccg ctacaccttg tacgactggt ggccccaggc
     3241 gggtggggtg atggggccag cgaccttctg gaacgtggac tacggcaccc gtgaccgtgt
     3301 ggccatcttt ggcgagtggg aggcgcagtg gaacgcgcag ttgttgagtc agtttggcat
     3361 ccggcatgag tccgtcaaga ccgatgcagg tccggtgcag ggctacaacg cccaggcgat
     3421 ctgggccagc gatgcagcag ccttcaatgc gcagagtcgc cagcgtaccg accagaacct
     3481 ggacctgaca gcgctggcac gttacaccga caacgcgacg cagacttttg aagccgggtt
     3541 ggctcgcaaa acccgctcgc ccaacctgta tcagcgctac ccctggtcaa cacagccgat
     3601 ggcaacgctg atgaacaatt tcgtcgggga tggcaatggc tacgttggca atctgaacct
     3661 gaagcctgaa gtcgcccaca ccctgagcgc cagcggcgac tggcacgatg ccgggcagc
//
LOCUS       DAOSLL010000498         3718 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_21766_length_3718_cov_4.636364, whole genome shotgun sequence.
ACCESSION   DAOSLL010000498 DAOSLL010000000
VERSION     DAOSLL010000498.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3718)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21766_length_3718_cov_4.636364"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="PLB25_17670"
     CDS             <1..556
                     /locus_tag="PLB25_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HOE43438.1"
                     /translation="ILDSSYFVVLRIRIRKSEVAIERTAQQAAKQQGLSAASRHQVAG
                     DLSVLVAVATKGGGRVNEHFGHVSEFQVFEVSANKARFVGHRRVDQYCQGGAGDDEQL
                     PSVVRAINDCHAVLVARIGACPKDELMAAGVEPVDSHVGEFIEKAALDWFNDYCGRIA
                     SGAVVHQSRGDAHIRQGAYTALAA"
     gene            591..785
                     /locus_tag="PLB25_17675"
     CDS             591..785
                     /locus_tag="PLB25_17675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HOE43439.1"
                     /translation="MTHKINTTTCTACDACEPECPNVAISVKKGVYIINATKCNDCEG
                     HFDDPQCVAVCPVDDCITEV"
     gene            814..1215
                     /locus_tag="PLB25_17680"
     CDS             814..1215
                     /locus_tag="PLB25_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HOE43440.1"
                     /translation="MLPNVTITPAAAKFINRIVRFAGLGAGAGLRLAVTPGGCSGYAS
                     EFSPEASVQPGEQLLEAGGVRLFLGAETRLLLDGITVDFVETATQSGLSFINPNKEAC
                     ACSSAETAVGGVTRIEISSIGRGRPAPVLAH"
     gene            1231..1836
                     /locus_tag="PLB25_17685"
     CDS             1231..1836
                     /locus_tag="PLB25_17685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43441.1"
                     /translation="MNANSLHFDDDLAAFADGVIGGGLSAQVNELITQAGLLRHLPQE
                     ALPLLEQARAAAPRHPAPLIALYRFHFYAHQLDLARAAGEDALAIARTALGPDFGDEP
                     PGDAATSGDAAVRFYLFTLKGLAYLNMRLGAMEEARLMLRELRRLDPQDHIGAALLFH
                     VLTRHESGGNAGASSDYPVRGWQLTSSPAAPSSPTQHWRKR"
     gene            1833..2237
                     /locus_tag="PLB25_17690"
     CDS             1833..2237
                     /locus_tag="PLB25_17690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HOE43442.1"
                     /translation="MSDINRDDTEIELAYPPRFGYGERVVARSAVRNDGTYNGRDIGD
                     VLVHKGEIGYVISIDTFLQQFYIYAVDFVESGHRVGMRAKELCTLDNLPPEVLAQLGA
                     RVSALKTLGSAQPDEPSGPNRRSATPASLTPN"
     gene            2249..2542
                     /locus_tag="PLB25_17695"
     CDS             2249..2542
                     /locus_tag="PLB25_17695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein NifZ"
                     /protein_id="HOE43443.1"
                     /translation="MNLNSLSAIEPREPAYAWGQRVIALDDLVNDGSYPDRAADALLA
                     ARGSVGEIVNIGHAPDVNEPVYLVQFDGCVVGCLEIELVPAPLGLLPETEALV"
     gene            2539..2889
                     /locus_tag="PLB25_17700"
     CDS             2539..2889
                     /locus_tag="PLB25_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43444.1"
                     /translation="MSRAAAAVLPAADQVLDLIAHRLARALRERLRYRYVHPRVTLQG
                     DGYRIQSPCCSRNVDPAGGLIDIALLVPQAGLRWSLCARDHASGTWVERFGNQPLDTL
                     LEVLCVDSDRQFWP"
     gene            2906..>3718
                     /locus_tag="PLB25_17705"
     CDS             2906..>3718
                     /locus_tag="PLB25_17705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOE43445.1"
                     /translation="MIYLDHNATTAPAPEVIAAMLPVLSGLWANPSSQHGPGQDAKRA
                     LNIARATVARMLGCKPVEVIFTSGATEANHLAVRGLLAAGRQTRRRVLFGSVEHASHL
                     RLARTLVDVGLQVDLMPVRRNGTLDLDAAAALIGPEVALVSLMAANNETGVLMPVAQV
                     AALAQAAGVLLHVDATQLIGKLPFQFASFGADAVSLSAHKFHGPKGIGALLLRQGVPC
                     QPQMPGSQERSRRAGTENLPGIVGMATALELCGDVSAEAVRLTGLRDALEHGL"
BASE COUNT          657 a         1150 c         1186 g          725 t
ORIGIN      
        1 catattagat tccagttatt ttgtggtttt acgcatacgg atcaggaaat ctgaagtggc
       61 gattgagcgc actgcccagc aagccgccaa gcagcagggc ctgagcgccg ccagccgcca
      121 tcaggtggct ggtgacctga gcgtattggt cgctgtagcc accaagggcg gcggccgggt
      181 taacgaacat tttggtcacg tcagcgagtt tcaggtgttt gaggtctcgg ccaacaaagc
      241 gcggtttgtc gggcaccgca gggtggacca gtattgccag ggtggcgcgg gtgacgatga
      301 gcaactgccc tcggtggttc gcgccatcaa tgattgccat gcggtgctgg tggccaggat
      361 tggtgcctgc ccgaaagacg aactgatggc cgccggtgtc gagccagttg acagccatgt
      421 cggcgagttc atcgagaaag ccgcgctgga ctggttcaac gactactgcg gccgcattgc
      481 cagcggtgct gtggtgcacc aaagccgtgg cgacgcccac atccgccagg gtgcctatac
      541 cgcactggcc gcctgattta ttcatttttt aacctgactg gagcatcacc atgacccaca
      601 agatcaacac caccacctgt accgcctgcg atgcctgcga gcccgaatgc ccgaacgtcg
      661 ccatcagcgt caaaaagggt gtctacatca tcaatgccac caagtgcaac gactgcgaag
      721 ggcactttga cgacccccag tgtgtcgcgg tctgcccggt ggacgactgc atcaccgaag
      781 tctgacacct gcccattgaa agccctgacc atcatgctgc caaacgtcac catcacgccc
      841 gctgcggcga agttcatcaa ccgcatcgtg cgctttgccg gtctgggcgc gggggcgggt
      901 ttgcgcctgg ccgtcacgcc gggtggctgc tcgggctacg cgtcggaatt cagccccgag
      961 gctagcgtgc agcccggtga gcaattgctg gaagccggtg gtgtgcgcct gttcctgggt
     1021 gccgaaaccc gcctgctgct cgatggcatc acggttgact ttgtcgaaac cgccacccag
     1081 tcgggcttga gtttcatcaa ccccaacaag gaagcctgtg cttgcagcag tgccgaaacc
     1141 gctgtcggcg gggttaccag aatcgagatc agctccattg ggcgcggtcg cccggcaccg
     1201 gtattggcac attgaaactg tcttgctgtc atgaatgcca acagcctgca ctttgacgat
     1261 gacctggcgg cgtttgccga tggggtcatt ggcgggggct tgagtgcgca agtcaatgaa
     1321 ctgatcaccc aggccgggct gctgcgtcat ttgccgcaag aggcattacc cttgcttgag
     1381 caggcgcgcg ctgcagcgcc acgccacccg gccccgttga ttgcgttgta tcgctttcat
     1441 ttttatgccc accagcttga cctggcccgc gccgccggcg aagacgcgct ggcgatcgcc
     1501 cgcaccgcgc tgggaccgga ttttggcgac gagccgcccg gcgacgcggc caccagtggc
     1561 gatgctgcgg tgcgctttta tctgttcaca ctcaagggcc tggcctatct gaacatgcgc
     1621 ctgggtgcga tggaagaagc ccggctgatg ttgcgcgagc tgcgccgcct tgacccacaa
     1681 gaccatatcg gggctgcctt gctgtttcat gtgctgacgc gccatgaaag cggtggcaac
     1741 gccggtgcgt cgagcgacta tccggtgcgc ggctggcagc tcaccagttc gccagcagcg
     1801 ccgtcttcac ccacccaaca ttggcgcaag cgatgagcga catcaaccgt gacgataccg
     1861 agattgagct ggcgtatccg ccacgctttg gctatggcga acgggtggtg gcgcgctcgg
     1921 cggtgcgcaa tgacggcact tacaacggcc gtgacattgg cgatgtgctg gtgcacaagg
     1981 gtgagattgg ctatgtcatc agcatcgaca cctttttgca gcagttctac atctatgcgg
     2041 tggactttgt cgagtccggc caccgggttg gcatgcgtgc caaggaactt tgcacgcttg
     2101 acaacctgcc ccctgaggtg ctggcgcaac tgggtgccag agtcagcgcg ctgaagacac
     2161 tcggatcggc acaacccgat gaaccaagcg gaccaaaccg ccggtctgct acaccagcca
     2221 gccttacccc caactgacca ggaacaccat gaacctcaac agcttgtcgg caatcgaacc
     2281 gcgtgaacct gcgtacgcct ggggccagcg tgtcattgcg ctggacgatc tggtcaacga
     2341 cggcagttac ccggatcgag ccgcagatgc gctgctggca gcgcgcggtt cggtcggcga
     2401 aatcgtcaac attggccacg cgcctgacgt gaacgagccg gtttacctgg tgcagtttga
     2461 cggctgcgta gtgggctgtc ttgaaattga actggtaccg gcaccactgg gcctgttgcc
     2521 agagaccgag gccttggtgt gagccgagcc gccgccgccg tgttgccagc cgccgatcag
     2581 gtgctggacc tgattgcaca ccggctggcg cgggccttgc gcgagcgact gcgctaccgc
     2641 tatgtgcacc cgcgcgtcac actgcagggc gacggctacc gcattcaaag cccatgctgt
     2701 tcgcgcaacg ttgacccggc tggcggcctg attgatatcg cgctgctggt gcctcaggcg
     2761 gggctccgct ggagcctttg tgcgcgtgac cacgccagcg gcacctgggt tgaacgcttt
     2821 ggcaatcagc cactggatac tttgctggaa gtcctgtgcg ttgacagtga ccgccagttc
     2881 tggccctgag ctgatgccga gcaccatgat ttacctggac cacaacgcca ccaccgcgcc
     2941 cgcgcccgag gtgattgcgg ccatgttgcc ggtgttgtct ggcctctggg ccaacccatc
     3001 atcgcagcac gggccggggc aagatgccaa gcgcgccttg aacatagcgc gcgccaccgt
     3061 ggcacggatg ctgggctgca agccggttga ggtcattttt accagcggtg caaccgaggc
     3121 caaccacctg gcggtgcgtg gcttgctggc ggccggtcgg cagacccgcc gccgcgtgct
     3181 gtttggcagt gtcgagcatg ccagccacct gcgactggcg cgcaccttgg tcgatgttgg
     3241 cctgcaggtg gacttgatgc cggtgcggcg caatggcacg cttgacctgg atgcggcagc
     3301 cgcgctgatc gggccggaag tggccttggt gtcgctgatg gccgccaaca acgagaccgg
     3361 cgtgctgatg cccgtggcac aggtcgccgc gctggcgcag gcggccggtg tgctgctgca
     3421 tgttgatgcc acccagttga ttggcaaact gccgtttcag ttcgccagct ttggtgccga
     3481 cgcggtgtcg ctttcggcgc acaagttcca tggccccaag ggcatcggtg cgctgctgtt
     3541 gcgccagggc gtgccttgtc agccccagat gccgggcagt caggaacgca gtcggcgcgc
     3601 tggcactgaa aacctgcctg gcattgttgg catggccacg gcgcttgagt tgtgcggcga
     3661 tgtcagcgcc gaagcggtcc gcctgaccgg gctacgcgac gcactggagc atggcctg
//
LOCUS       DAOSLL010000499         3716 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_21781_length_3716_cov_3.325321, whole genome shotgun sequence.
ACCESSION   DAOSLL010000499 DAOSLL010000000
VERSION     DAOSLL010000499.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3716)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3716
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21781_length_3716_cov_3.325321"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLB25_17710"
     CDS             <1..493
                     /locus_tag="PLB25_17710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOE43446.1"
                     /translation="LRVLARRSDGQAGLAAEPMNPRVAAMLSREDNVGMRSAAFYSDL
                     QRQAIRIKRELLLFLLKAQASGETVAAYGAAAKGNTLLNFAGVRADLVPYVVDRNPAK
                     QGKFLPGSRIPVRAPEYLLAHRPEYVLILPWNLKKEVMEEVGAAVDWAGRFVVAVPTL
                     QIV"
     gene            574..1692
                     /locus_tag="PLB25_17715"
     CDS             574..1692
                     /locus_tag="PLB25_17715"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006387498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HOE43447.1"
                     /translation="MNTERIALSRPSITALELSCMADAAANGWGEHCYDYIEQFQRTF
                     QQALNVPYAITASSCTGALHMGLAALEIGAGDEVILADSNWIATVAPVVHLGAQPVFV
                     DILADTWCIDPDGVETAITPRTRAIIATHLYGSVCALARLQQIADRHGLVLIEDAAEA
                     IGSEYQGQAVGTFGRFGCFSFHGSKTICTGEGGMLVTRDADFYDKVLTLSNHGRRKGQ
                     QRQFWPEVIGFKYKMSNLQAALGCAQTQRMAELVERKRQILAAYRLLLQESDGVKMNV
                     EHPGTRIGAWMPTLVFSPQTGMTARRMVDEFQSRSIDGRVFFHPLSSLPSFGLQAGQP
                     VAADIARRAVNLPSFHDMTDAQIERVTQTVKAALHAGC"
     gene            complement(1997..2329)
                     /locus_tag="PLB25_17720"
     CDS             complement(1997..2329)
                     /locus_tag="PLB25_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43448.1"
                     /translation="MVAPVYRCWISNHGVASGDVMRHHGTRTDHRPITDMEARQHHRT
                     CTYKHIVAHHHLTAQSGTWRHMHSITQLTLMIDHSGVVHDTHPAQSSICGDHGHWQDM
                     TASPKVCR"
     gene            2379..3105
                     /locus_tag="PLB25_17725"
                     /pseudo
     CDS             2379..3105
                     /locus_tag="PLB25_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004631452.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cephalosporin hydroxylase family protein"
     gene            complement(3110..3523)
                     /locus_tag="PLB25_17730"
     CDS             complement(3110..3523)
                     /locus_tag="PLB25_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43449.1"
                     /translation="MQWLAEERFPGQQMQQVHYVEQSWLGLPPEWMTQATPGVGLKPG
                     LRVALAHAQGLMEKALQQAKDADLKTAQASQKRPHPYWNDLTTLCDTVLASVPNEPFS
                     LACLAEVEHASGHTDFAVTYLRYAQTAIELNTQPS"
BASE COUNT          756 a          943 c         1123 g          894 t
ORIGIN      
        1 cctgcgggtg cttgcgcggc gcagcgatgg ccaggctggc ctggccgcag agccgatgaa
       61 tccgcgagtt gctgcgatgc tctcgcgtga agacaatgtc ggcatgcgca gtgccgcgtt
      121 ttacagtgat ttgcagcgcc aggccatacg tataaagcgt gagctgctat tatttttatt
      181 aaaagcgcag gcatccggtg aaactgtggc tgcctatggt gcagcggcca agggcaatac
      241 gctgcttaac tttgctggtg tgcgtgccga ccttgtgccc tatgtggtgg acaggaaccc
      301 ggccaagcag ggcaagtttc tgccaggcag ccgtataccc gtgcgggcac cagagtattt
      361 gctcgcccat agacctgagt acgtgctgat tcttccctgg aatctcaaga aggaggtgat
      421 ggaagaggta ggtgctgcgg ttgactgggc agggcgcttc gtggtggcag tgccgaccct
      481 gcagatcgtc tgattccacc accattagac catctgacag atgaagcaga ttgccaagct
      541 ccagtttaaa tactacctct ccaggacgtt gacttgaata cagaacgtat tgcgctttca
      601 aggccatcca tcactgcgct ggaattgtcc tgcatggccg atgccgccgc caatggctgg
      661 ggcgagcatt gctacgacta cattgagcaa tttcaacgga cgtttcagca ggcgctgaat
      721 gttccgtatg caattacggc ctcaagttgc acaggggcct tgcatatggg tttggccgca
      781 ttggagattg gcgctggaga tgaagtcatc ttggccgaca gcaactggat tgcgaccgtt
      841 gccccggttg ttcatctggg cgcgcagcca gtttttgtcg atattctggc agacacttgg
      901 tgtattgacc ctgatggtgt cgagacggcc atcacgcccc gtacccgtgc catcattgcg
      961 acccacttgt atggcagcgt ctgtgcgctt gcccggcttc agcaaatagc ggaccgacat
     1021 ggtctggtcc ttatagagga tgctgcagaa gccattggct ccgaatacca ggggcaggcg
     1081 gtgggaacct tcggtcgctt tggctgtttt tcgtttcacg gctccaagac catttgtacc
     1141 ggtgaaggcg gcatgctggt gacgagggac gctgattttt acgacaaggt actaaccctg
     1201 tccaaccatg gacgacgaaa agggcagcag cgccagtttt ggccggaggt gattggattc
     1261 aaatacaaga tgtcgaatct gcaggccgct ttgggttgcg cccagacgca acgcatggcc
     1321 gaactggtcg agcgaaagcg ccagattctt gccgcctacc gtcttctgct gcaggagtcg
     1381 gacggtgtga agatgaatgt ggaacatccc ggcacacgca ttggtgcctg gatgccaacg
     1441 ctggtgttca gcccgcaaac tggcatgacc gcgcgcagaa tggtggatga gtttcagtcg
     1501 cgctccatcg atgggcgggt gttttttcat cctttgtcct cattgccatc ctttgggtta
     1561 caggctgggc aaccggtggc cgcagacatt gccagacgcg ccgtcaattt gcccagcttt
     1621 cacgacatga ccgatgccca gatcgagcgg gtcacgcaaa cggtgaaggc agctttgcat
     1681 gccggctgtt gatcgattcg tatgttgggg ggcggcgggt catgccaggg tactgcacga
     1741 tctgttggca gaaagtggta ccacctgcgt ggcctttatc gacgcggccg tgaaaactca
     1801 gacatttctc ggctgtccgg tttttcggga tgttgatgag tttcaggcat ggtggcgaac
     1861 acgttctgat ttggacgact tgaaagccat gtttgcaatc ggtcgccagg gcgggcaccg
     1921 cttggcggca ttggaacggg cacagtccct ggggctgcgg gtgccggcca tttgcagcgt
     1981 tcaagcgcat gtcagttcat cggcatacct tggggctggc agtcatgtct tgccaatggc
     2041 cgtggtctcc gcagatactc gattgggcag ggtgtgtatc gtgaaccacg ccgctgtgat
     2101 cgatcatgag tgtcagttgg gtgatggagt gcatgtggcg ccacgtgcca ctttgtgcgg
     2161 tcaggtggtg gtgggccacg atgtgtttgt aggtgcaggt gcggtggtgc tgccgcgcct
     2221 ccatatcggt gatcgggcga tgatcggtgc gggtgccgtg gtgacgcatc acgtcgccgc
     2281 tggcaacacc gtgattggaa atccagcatc tgtataccgg agcaaccatg acaattccag
     2341 atgatttttt aaaagagcgt gagcaacaaa tccgcgatat gacgcaagat accggattcg
     2401 tgacactgtc tcagcggtgg ttgcaggaat ccatgcagcg gcgctatgtg tacaactttg
     2461 actggctggg gcgtccgatc attcaatacc cgcaagacat ttgttgcggt gcaggagctc
     2521 gtgtggcgtg ttcagcccga tctgattgtt gagactggta tcgcgcatgg tggttcactg
     2581 atacttagtg ccagtatgtt ggcgcttctt gatgtgtgtg atgcaagtgt gcagcgctgc
     2641 gccatggatc cgagtgccag tcatcgcaaa gtggtcgggg tggacattga tatcaggcag
     2701 cacaaccgca cggccattga ggctcatccg cttgcgccct acatcgagat ggttcaaggt
     2761 tcctcggtgg cgggcgaaac gatacaggcg gtacagcggc tggcacagaa tcattcaaag
     2821 acgttggtct ttctggattc catgcacacg catgaccacg tacttgcgga gttggacgct
     2881 tacgcacccc tcgtgagttc gggcagttac tgcgtggtgt ttgatacctt tgtgcagaac
     2941 atgccacctg gtttttttgc agaccgtccc tggaacgtgg gtgacaaccc catgacagca
     3001 gtttcgcaat ggctagggca acatcatgag tttgaggtgg ttgctgattg gccggacaag
     3061 ctgatggtga ccgtcgctcc aggcggattt ttgcgacgga aatgacggtt tagctcggtt
     3121 gcgtattcaa ttcaatggcc gtctgggcgt agcgcagata ggtcaccgca aaatcggtgt
     3181 gaccggaggc atgctccacc tcggccaaac aagccaacga aaatggttcg tttggaaccg
     3241 atgccagtac cgtgtcgcac aaagtggtta agtcattcca ataggggtgc gggcgtttct
     3301 gtgaggcttg agcagtcttg agatctgcat ccttggcttg ttgtagtgcc ttttccatca
     3361 atccctgggc atgggccagt gcaactcgca gcccaggctt aagaccaacg cctggtgtag
     3421 cttgcgtcat ccattctggt ggcaagccaa gccatgactg ttcgacatag tggacttgct
     3481 gcatttgttg gccagggaaa cgttcctcgg ccagccactg caaccaaatt ttgcgcaaac
     3541 tcaacccaaa tcgatggcaa aagcggtctt cccgcatcag gatggactgc tcaacttcgt
     3601 ttctcaaacc catgcgtacg gtgttgagtt gcgcaatatc catatcaaag tgacaagact
     3661 atttgatgta gctttgacga tcattggtca gccactcagg acgccctagg tgaagt
//
LOCUS       DAOSLL010000500         3712 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_21820_length_3712_cov_3.540607, whole genome shotgun sequence.
ACCESSION   DAOSLL010000500 DAOSLL010000000
VERSION     DAOSLL010000500.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3712
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21820_length_3712_cov_3.540607"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..99
                     /locus_tag="PLB25_17735"
     CDS             <1..99
                     /locus_tag="PLB25_17735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HOE43450.1"
                     /translation="LFDAGGAICQASRKFLQSWMDLYVVWVKKHAA"
     gene            126..380
                     /locus_tag="PLB25_17740"
     CDS             126..380
                     /locus_tag="PLB25_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2789 domain-containing protein"
                     /protein_id="HOE43451.1"
                     /translation="MDKAIHHFNELFAQLGLPDDAQSIANFLASHTSMSACVRLPDAP
                     YWTPAQAQFLRESLQQDSDWSGLVDQLGKALQGEPHRPCN"
     gene            complement(390..644)
                     /locus_tag="PLB25_17745"
     CDS             complement(390..644)
                     /locus_tag="PLB25_17745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-like protein"
                     /protein_id="HOE43452.1"
                     /translation="MKYSELLIAGTLSFVLAGCISAPAGPPGPQGATGATGAPGDVGY
                     TGATGRTGATGYTGATGDTGAKGNTGSTGTSGTVIVVPPQ"
     gene            1069..2760
                     /locus_tag="PLB25_17750"
     CDS             1069..2760
                     /locus_tag="PLB25_17750"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE43453.1"
                     /translation="MTSAIWILLIAAIFATAVLTQRSLSRLADSREVITHSLVINKAL
                     NDMMSQMLDAETGQRGFLLSGRAPYLQPYYTALAQVRQSRAALGAALSQDSTALAPVD
                     VLDKAIATKLQDLDHAVSLKSEGRSDEAVQLILTDSGNQTMNAVRAAVRSLDSNQVRR
                     TNLLQAEIEREIRDYYLILGVSILVNLILLVGLIQRFRNASIQRDAAQQVLDDRNVEL
                     SRLLAAAATRNAQVQGLSELSRFLQSCADIDEAAGLLKQHLPPLMRAASGSLYLAAVE
                     QDSLRQAFTWGDKTFVESFEISECWAARLRQPFRQPFETGTASCAHLQSEYASSHNNN
                     NIQCLPLLAYGELLGIVVLEADMVSAPQESLEIEGSRRFALEQVGLSIGNLKLRESLR
                     QASIRDILTGLYNRRFFDESSQREVVRALRQQTKGDYAGLALMMIDIDHFKRFNDQHG
                     HEVGDQVLCEVAQVLQSQTRGSDVAARFGGEEFTIVLADITAPRALERAERVRQAVEQ
                     LVLRPSGNDLGTITISIGMSQFPKNGTTVESLLQAADKAPYEAKDSGRNRVVVAG"
     gene            complement(3027..3164)
                     /locus_tag="PLB25_17755"
     CDS             complement(3027..3164)
                     /locus_tag="PLB25_17755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43454.1"
                     /translation="MSAMEVGSGTLLGTSGAALSFQGAAQPESVVMMPGKFTLRLKFY
                     P"
     gene            complement(3340..>3712)
                     /locus_tag="PLB25_17760"
     CDS             complement(3340..>3712)
                     /locus_tag="PLB25_17760"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HOE43455.1"
                     /translation="MAIVSDAQTAYALPELNTVGLAGYFDPIIVSGDFGYRKPDVRLF
                     EAALNAMKLESSEVLFVGNDLYRDVHGPQKLGIKTVFFKSSDMTQDKVQATPDYIIYH
                     FPELLNAVRFFEDVDRRQTER"
BASE COUNT          781 a         1046 c         1098 g          787 t
ORIGIN      
        1 ttgttcgacg ctggcggcgc aatctgccag gccagcagaa agttccttca gagttggatg
       61 gacctatacg tggtctgggt caagaagcat gcagcctgat caacacaaac gaccgagtca
      121 acaccgtgga caaggcgatt caccacttta acgagctgtt tgcccaactg ggcctacccg
      181 acgatgctca gagcatcgcg aattttctgg ctagccacac ctccatgtca gcctgcgtga
      241 ggctgccaga cgcaccctat tggacccccg cgcaagccca gtttctcagg gagtcgctgc
      301 agcaggattc agactggtct ggcctggtgg accaactcgg caaggcgctg cagggtgaac
      361 cacaccgacc ctgcaattga ccagccggtt tactggggcg gaacaacgat gacggtgccc
      421 gaggtgccgg tggaaccggt attgcccttg gcaccagtgt caccggtcgc accggtatag
      481 ccggtggcac cggttctgcc agttgccccg gtgtagccga catcgcctgg cgcaccggtg
      541 gcaccggtgg caccttgggg gccgggtggc ccggctggtg ctgaaataca tccggccaac
      601 acgaatgaga gggtgcctgc gatcagcagt tctgaatatt tcatggggat tcctttggag
      661 tgaatgacgg ttttgatcag gttctggcaa gaagctcagc atcggcagat cagactgttg
      721 ctggcgcagc cgctttgtgg tgttgcgact ggtgccgaga ttgatggatt ggccagagta
      781 cgtcagtgcg ctggcccaca gacaccgatg caaggtgatg tcacagtcgc tcaaagggcg
      841 cgggtggctt tgatggctga acggctattt gcacggcccc gttcaaagac atgatcagtg
      901 cccaagcact gtttgaccct gttcaatttg gctatgagat gtcagcttct ggtcgggcac
      961 aggcttctcg ttcaattctc tgcattcccc agccaccgtt tgacgaatca aattccggag
     1021 tcgcccatgt tgcaagcaag gactgatcaa aatcgacatt acacaagctt gacatcggcc
     1081 atttggatcc tgctgatcgc ggccatcttc gccaccgccg ttttgaccca gcgcagtctg
     1141 tcgcgcctgg ctgactcacg agaggtcatc acccattcgc tggtcatcaa caaggcgctc
     1201 aatgacatga tgagccagat gctcgacgct gaaaccggac agcgtggttt tctgctcagt
     1261 ggccgtgccc cctatctgca gccctactat acggcgctgg cccaggtgcg gcaatcgcgt
     1321 gccgcactcg gtgctgccct cagccaggat tcaaccgccc tggcaccggt tgatgtgctg
     1381 gacaaggcga ttgcgaccaa gttgcaagac ctggaccatg cggtgagtct caagagtgag
     1441 ggacgatccg acgaggcagt tcagttgata ctcaccgact cgggcaatca gaccatgaac
     1501 gcggtgcgag cggcagttcg tagcctggac tccaatcagg ttcgccgcac caacttgctt
     1561 caggccgaaa ttgagaggga aataagagac tactatttga ttcttggtgt gtcgattttg
     1621 gtcaatttga tcttgcttgt cggcttgatc cagcgttttc gaaacgcttc aatccaaagg
     1681 gatgccgctc agcaggtgtt ggatgatcgc aatgtcgagt tgtcgcgact gctggccgca
     1741 gccgcgaccc gcaatgcaca ggtgcagggc ctatccgagt tgagtcgttt tttgcagtcg
     1801 tgcgcagaca ttgatgaagc ggcgggcttg ctcaaacagc accttccgcc cttgatgcgg
     1861 gctgccagcg gatcactgta tctggcagcc gttgagcagg actccttgcg ccaggcgttc
     1921 acctggggag acaagacatt cgtcgaatcg tttgagatca gcgaatgttg ggccgcgcgc
     1981 ctcaggcaac cgtttcgcca gccttttgag accggcacag cctcctgcgc ccatctgcaa
     2041 tccgagtatg cgagttcaca caacaacaac aatattcagt gcctgccgtt gctggcctac
     2101 ggtgaattgt tgggcatcgt ggtgctggag gcggatatgg tgagtgcccc gcaagaaagc
     2161 cttgaaatcg agggcagccg acgctttgcg cttgagcagg tcggcttgtc cattggcaac
     2221 ctgaaactgc gcgaatcatt gcgccaggca tccatacgcg acatattgac cgggctctac
     2281 aaccgccggt tctttgatga atcatcgcag cgtgaggtgg tgcgcgcgct gcgccaacag
     2341 accaagggcg actatgcggg cctggcgctg atgatgattg atattgacca cttcaaacgc
     2401 tttaacgatc agcacggcca tgaggtcggc gatcaggtgt tatgcgaagt agcgcaggtc
     2461 ttgcaaagtc aaacgcgcgg cagtgacgtg gctgcgcgtt tcggcggcga ggagttcacc
     2521 attgtgttgg cagacatcac ggcaccgcgc gcattggagc gcgccgaacg ggtgcgccag
     2581 gcggttgaac aacttgtact ccggccatca ggaaacgacc tgggaaccat cacgatatcc
     2641 attggcatgt cgcaatttcc aaaaaacggc acgacggtgg agtccttgct ccaggcggct
     2701 gacaaggcgc cttatgaagc caaggacagc ggtcgcaatc gggtggtggt tgctggctga
     2761 gcatggggca tctcaccgga gcagggggat tggccacgat tccagccaaa tccatgtgag
     2821 cgcagttcgc ctggtggcac ccgactgatg tcggtgcaag ggctccgatg aaggcgggac
     2881 gttcgcacgg tggcagtcca tgtgagggta acctgggtga ctcaagcacc tgggtttagt
     2941 ggatccccac ctataacctg ttggattcag gcagcggctt gtgccagcaa aatcctgttt
     3001 accgtgccaa ccggcacgca gctttctcag ggatagaact tcaatctcaa cgtgaacttc
     3061 ccgggcatca tcaccaccga ctcgggttgg gctgccccct ggaatgacag ggcagcacca
     3121 gaagtgccca gtagcgtgcc agagccgacc tccatcgcgc tcatgggagt gcatgcgacc
     3181 aaaccgggcg ctgcctgatt cttgggatgt cacctgcacc tttcggggca ggcgcagcgc
     3241 gttcaacgga ccgcgccacc aggacccacc gcaactgcca atgaacaggg cgaaccccgg
     3301 tctatatgcc cttcgtgcca gatcgctggc atggcggttt caacgctctg tttgccgccg
     3361 atcaacgtcc tcgaagaagc gcacagcgtt gagtagctcg ggaaagtggt aaatgatgta
     3421 gtcgggcgtt gcctgcactt tgtcttgcgt catatcgctt gatttgaaaa aaaccgtctt
     3481 tatcccgagt ttctgtgggc cgtggacatc ccggtacagg tcgttgccga caaacagaac
     3541 ttccgaggat tccagcttca tggcgttcag tgctgcttcg aacagccgca catcgggctt
     3601 gcgatagcca aagtcgcctg acacgatgat cgggtcaaaa taaccagcaa gacccaccgt
     3661 gttgagctca ggcaaggcgt aggcggtctg tgcatccgag acgattgcca tg
//
LOCUS       DAOSLL010000501         3687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_22025_length_3687_cov_4.510463, whole genome shotgun sequence.
ACCESSION   DAOSLL010000501 DAOSLL010000000
VERSION     DAOSLL010000501.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22025_length_3687_cov_4.510463"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /locus_tag="PLB25_17765"
     CDS             <1..1438
                     /locus_tag="PLB25_17765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HOE43456.1"
                     /translation="LGNIGQLRHGNGQKIHGQSYRLTMKIAGRDDKVVIGAHRGIVGR
                     GVDLEQAKGRLALAEYNLLVELTNLHDVSARYLRVVGEKPPAILPYLPEQFKFASMPV
                     SLEVLMRDGLQNSPTLNAAVENALSYKEAIETRKSAYMPRIDLRAYQSRESNTSGVIG
                     ATRNHGIELLLSYNLYRGGADRARQRQAVDQGEQARDLQAKACRDVRQTLSIAYSDVQ
                     SLSAQMGYQDAHRLATEKSLQAYRQQFELGQRTLLDVLDSQNEFFEATRSYINSRYNQ
                     AVAQARTLAGMGQLVTVMGINRADVPSAKDAGQERQQIDPAELCPMEETVVDSIEKIK
                     AQVVLPPRLTKLAAPVAAAPEALKTTKVRLAADALFDFNQSELKPGGLAGLDDLIKSI
                     KGMQLDVVIATGHTDSVGSDEYNHKLSLARAEAVKTYLVSKGIDVKRVRAIGQGQRQP
                     VGDNQTTEGRAKNRRVDVDVVEAAPAAR"
     gene            1548..1997
                     /locus_tag="PLB25_17770"
     CDS             1548..1997
                     /locus_tag="PLB25_17770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HOE43457.1"
                     /translation="MSKAKAMVSAVGASVDDAEANFYEALQTADLERLMACWADDDDV
                     FCVHPGGPRLVGLGAIRAAFDAMFGNGSLRIQPEAVRKIDNMTSAVHSVRERIEMITN
                     EGPIAAYVLATNVYLMTAQGWRLVAHHASPGADHEAQDIRELPPVLH"
     gene            2036..3031
                     /locus_tag="PLB25_17775"
     CDS             2036..3031
                     /locus_tag="PLB25_17775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HOE43458.1"
                     /translation="MTYVAPCWLPGGHAQTIWPALYVRHGPADLRQIRTLYQRERWTA
                     PDGDFIDLDWLAGPPGSSLLVLFHGLEGSSGSHYAMAFAQFAQAHDLSFVVPHFRGCS
                     GELNLAPRAYHSGDFEEIGWVLQRLRQRHGGPLLAAGVSLGGNALLRWAEEMGSQAAG
                     VVAALSSISAPLNLAVSGQAMGRGFNRQVYTRMFLASMKPKALRKLAQHPGLFSAKAL
                     CAARDLFDFDNVFTAPLHGFKDTPDYWKRASAQPHLDQIQVPSLLVNALNDPFVPASS
                     LPQDANVSRYVTLWQPDQGGHVGFPMGRPPSHLRALPDIVGGWLQSHLPPISAVT"
     gene            3050..3589
                     /locus_tag="PLB25_17780"
     CDS             3050..3589
                     /locus_tag="PLB25_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007868926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2946 family protein"
                     /protein_id="HOE43459.1"
                     /translation="MDDIVKQAMAKWPSVPDCYGWLGLGARGNWYMRDDRVQALGQFA
                     SGVPGCKGSLLQHDKLIEFIGRNYAADAQGQWFFQNGPQRVYVELEATPLVWRIQPDL
                     SVRDHIGRSALVQDALVDELGHLYLATDRGFGLVHTQDVGLAADAIEQGLWRVQEDTQ
                     ARLPVRFGYLKSPQSLQNK"
BASE COUNT          786 a         1032 c         1089 g          780 t
ORIGIN      
        1 actcggaaat attggccaac tcagacatgg caatggccaa aaaatccatg ggcaaagcta
       61 tcggctcacc atgaaaattg ccggccgaga tgacaaggtc gtcatcgggg cacaccgtgg
      121 gattgtcggt cgtggcgttg atctggagca ggccaagggc cgtctggcgc tggccgaata
      181 caacttgctg gttgaactga ccaacctgca cgatgtcagc gcacgatatt tgcgcgtggt
      241 cggggaaaag cccccggcca tcctgcccta cctgcccgag caattcaagt tcgccagcat
      301 gcccgtttca ctggaggtat tgatgcgcga cggtctgcaa aacagcccta ccctcaacgc
      361 cgctgtggaa aacgcgctct cttacaagga ggccattgag acgcgcaagt ccgcctatat
      421 gccccgtatt gatttacgtg cctatcagtc acgcgaaagc aataccagcg gggtcattgg
      481 ggccacgcgt aaccatggca ttgagttgct gctgagctac aacctgtacc gcggcggtgc
      541 ggaccgggcc cgccagcgcc aggcagtgga tcaaggcgag caggcccgtg atctgcaggc
      601 caaggcctgt cgtgatgtgc ggcaaacgct gtcaattgct tacagtgatg tccaaagcct
      661 gagcgcccag atgggctacc aggatgctca ccgcctggcg acagaaaagt cccttcaggc
      721 ttatcgtcag cagttcgaac tgggtcaacg tacattgctc gacgtgcttg acagtcagaa
      781 cgagtttttt gaggcaaccc gctcttacat caattcgcgt tacaaccagg ccgtcgcgca
      841 ggcgcgcact ttggctggga tggggcaact ggtcaccgtc atgggcatca accgtgctga
      901 cgtgccaagc gccaaggatg ccggtcagga gcgtcaacag atcgaccccg ctgaactgtg
      961 cccgatggag gaaaccgtgg tggacagcat tgaaaaaata aaggcccagg ttgtattgcc
     1021 gcctcgcttg accaaacttg ctgcgcctgt tgcagccgct ccggaggcac tcaagacaac
     1081 caaagtgagg ctcgcagcag acgcgctatt tgatttcaac cagtccgaac tcaagccagg
     1141 cgggctggcc gggcttgatg acttgattaa gagcatcaaa ggtatgcagc ttgacgttgt
     1201 cattgcgacc ggccacaccg attcagtcgg cagcgatgag tacaaccaca agttgtcgct
     1261 ggcacgtgcc gaagcggtta aaacctatct ggtgagcaaa ggtatcgatg tcaagcgggt
     1321 tcgggccatc gggcaaggcc agcgccagcc ggtgggagac aaccagacca cagaaggacg
     1381 tgccaaaaat cgccgcgttg atgtggacgt ggttgaggcg gctccggctg caaggtgatt
     1441 tgttgcgaca gcagcgcgcc cgattgaatc aggcgcaagt tttgcaattt tcgattgtcg
     1501 gccttcaaac cgggtcaaaa tcgtgatttg caaaacgatc aacaaccatg tcaaaagcaa
     1561 aagcaatggt cagtgccgtc ggtgcttctg tcgatgatgc cgaggcgaat ttctatgaag
     1621 cgctgcaaac cgccgacctt gaacgcctca tggcctgctg ggccgacgat gacgatgttt
     1681 tctgtgtcca ccctggtggt ccccggctgg tcgggctagg tgccatccgg gctgcttttg
     1741 atgccatgtt cggtaatggc agcttgcgca tccagcctga agcggtgcgc aagatcgaca
     1801 acatgaccag cgccgtccac agcgtgcgcg agcgtatcga aatgatcacc aatgagggac
     1861 caatagcagc gtatgtgttg gccaccaatg tctatttgat gactgcgcaa ggctggcgat
     1921 tggtagcgca ccacgctagc ccgggggctg atcatgaggc gcaagacatc cgtgaacttc
     1981 cccctgtttt gcattgaatt tgcctccagc ccttaccagt aatgaacaag cagctatgac
     2041 atacgtagcg ccctgctggt tgcccggcgg tcatgcgcaa accatatggc cagcacttta
     2101 tgttcggcac ggacccgccg acttacgaca aatacgcacg ctctaccagc gtgagcgttg
     2161 gacagcaccg gatggcgatt ttatcgatct cgactggctt gccggtccac ccggcagcag
     2221 cctgctggtg ctgtttcatg ggctggaagg ctcttcaggt agccactatg ccatggcgtt
     2281 tgcccagttt gctcaagcgc atgatctgag ttttgtcgtg cctcattttc gtggctgtag
     2341 cggcgagctc aacctggcac ccagagccta ccattctggt gattttgaag aaatcggctg
     2401 ggtgttgcag cgtctgcgcc agcggcatgg cgggccgttg ctggctgccg gggtttcgct
     2461 gggaggtaac gctttgctgc gctgggccga agaaatgggt agccaggccg ccggagtggt
     2521 tgccgcgctg tccagcattt cggcaccgct gaacctcgcc gtcagtggtc aggcgatggg
     2581 acggggtttt aatcggcagg tttatacccg catgtttttg gccagcatga aacccaaagc
     2641 gctgcgcaaa ctggcccaac atccgggttt gttcagcgcc aaagccctgt gcgcggcgcg
     2701 cgatcttttt gactttgaca acgtgtttac agcaccgtta catggcttca aagacacgcc
     2761 cgactattgg aagcgcgcct cagcacaacc ccatctggac cagattcagg tgccgtccct
     2821 gctggtcaat gcgctgaacg accctttcgt accggcaagc agtctgccgc aagacgctaa
     2881 cgtgagccgc tatgtcacac tttggcagcc cgaccaaggc gggcacgttg gttttccaat
     2941 ggggcggcca ccgtcccatc tgcgggcctt gccggatatc gtgggtggtt ggctgcaatc
     3001 gcacttgccg cccatttccg ccgttacttg aagtatagga atatgcccga tggatgacat
     3061 cgtcaaacaa gccatggcca aatggccatc cgtgccagac tgctacggct ggcttgggct
     3121 gggtgcacgc ggcaactggt acatgcgtga tgaccgagtg caggcgctgg gccagtttgc
     3181 cagcggtgtt cccggttgca agggttcgtt gttgcagcat gacaagctga ttgaattcat
     3241 cggccgcaat tacgctgccg acgcgcaggg tcaatggttc ttccagaatg gcccgcaacg
     3301 ggtgtacgtc gaactggagg ccacgccctt ggtctggcgc attcagccgg acttgagcgt
     3361 gcgagaccat attggccgca gcgctctggt ccaagacgca ctggttgacg aactcgggca
     3421 cctttatctg gcaaccgatc gcggctttgg tttggtgcac acccaggatg ttggcctggc
     3481 agcagatgcc atcgaacagg gtttatggcg agtgcaggag gatacgcaag cccgcctgcc
     3541 ggttcggttc ggttatctga aaagtccgca atcgctacaa aataagtagc tggcagcgct
     3601 ttctgaatat aggctacagg acgttttgac caatgaagtg agtgatcatt tggtgcccga
     3661 agccgccgct tgtggcgcag cagcaac
//
LOCUS       DAOSLL010000502         3685 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_22043_length_3685_cov_4.841598, whole genome shotgun sequence.
ACCESSION   DAOSLL010000502 DAOSLL010000000
VERSION     DAOSLL010000502.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22043_length_3685_cov_4.841598"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(264..338)
                     /locus_tag="PLB25_17785"
     tRNA            complement(264..338)
                     /locus_tag="PLB25_17785"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(304..306),aa:Val,seq:cac)
     gene            complement(853..2103)
                     /gene="lysA"
                     /locus_tag="PLB25_17790"
     CDS             complement(853..2103)
                     /gene="lysA"
                     /locus_tag="PLB25_17790"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HOE43460.1"
                     /translation="MNPFQPNTLASLAQTHGTPLWVYDAATIEHQIGALRQFDVIRFA
                     QKANSNTHILRQMRHLGVMVDAVSLGEIERALVAGYRPGVHNGHAEIVFTADLLDRAT
                     LPRVASLGIPVNCGSIDMLDQLGAVSPGHPVWLRINPGFGHGHSNKTNTGGEHSKHGI
                     WHTDLPLACARLRANGLTLVGLHMHIGSGVDYSHLEQVCGAMVALVTTVHEQGMDLVA
                     VSTGGGLSVPYQADEPTVDTRHYFQLWDAARRQIADLLGHPVKLEIEPGRYLVADSGV
                     LLTEVRATKQMGSNAFVMVDAGFNDLMRPSMYGSFHGMALIHADGTVVDGPLQATVVG
                     GPLCESGDVFTQGEGGVVLTRALPLARVGDLLVLHDTGAYGASMSSNYNTRPLIAEVL
                     VENGVPRLIRRHQSVTELLALELV"
     gene            complement(2221..>3685)
                     /locus_tag="PLB25_17795"
     CDS             complement(2221..>3685)
                     /locus_tag="PLB25_17795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HOE43461.1"
                     /translation="NASAWAGVNIADAIIYELHVGCFTPEGTLAAATAKLADLAALGV
                     TVIELLPLAAFPGERNWGYDGVAQFALHQAYGDYADLKALIESAHALGMAVILDVVYN
                     HFGPEGNYTGALAPYTKPAATPWGAAINFDASYNYGIREFFLANIQYWLQQVGFDGLR
                     MDAVSLVFDNSPVHILREFTDLAQRIARQEGREILMIAEHLRNNRFVTHPDGFNYQSQ
                     WSDDLNHAVFALLTGETRRHYGDFGSFDDVVKALQQGFVFDGSRLERVHNTMMGTDGR
                     LTQAQQHVVHMQNHDQVGNRPQGDRMMASYGRSRALLGITAVFASPFVPMLFMGEEYG
                     ETAPFLFFVDFQDPRLIEAVRKGRKRDCAFGSSSPPEAHVRSSFEASKLQWTRRMSAQ
                     GQAIWHHYRALIALKRSGALGPRDMSSVKVSGERSTGVITLEAPQTLTVLNFSPQPQK
                     FVVPEGWQLILNADLDDPETRYCGNLSAYGAVIFSRV"
BASE COUNT          825 a         1127 c         1023 g          710 t
ORIGIN      
        1 caagcgtctt gcaacgacgc tgtgcgccca aatccgccgg tgcaaatagg ggaaattatt
       61 tctgaccaca tccttacctc tttgattgcc tgctgcctgg accacgagct acccggccat
      121 gcggcatctc gccaaccaac tcgctgaagg aacaacgcac gaggcgacaa atgcgccttc
      181 aaagccatga aggcatcagt cgttgattta ccaattaaaa aagcctccag gaattgctcc
      241 ttaaaggcct ttgaacttgc aattggtggg cggtgcaggt ttcgaacctg cgacccccac
      301 agtgtgaatg tggtgctcta cccctgagct aaccgcccaa ttgcgctaac caaaaaaata
      361 cgccggacaa caagaaccgc caagcgccac cagggtctgg cagcagcgcg cgatttcttg
      421 atttttgccc atctgaaacc ccaccgcaac atttcccgat ggattgaaac aggccggatt
      481 atgccatggg tgcagcttgg tccgatgggc gatcgcccag ggccaatcac actctgcacg
      541 acttcaaggt caacaaccag caggtgccgg gcaagccttg ggacaaattc cactacttga
      601 acccccgtca gcggcagtgc ttgatgcgta ggtgccaaac tcacataact ttggcggtcg
      661 ttggcaccta cctcaaattc aatttctgag tcattcgtgc ctaggcacct tggcgcagcg
      721 caagaaaaat acctcaaacc attaaaattc atagcgttgc acgcttacag gacgggggct
      781 agaggcaaca aatgcgtcaa atgccgctca gaccaggcac ctgcatggcc tgccagcgat
      841 ggcccaatca actcaaacca actccagcgc cagcaattcc gtcacggact ggtgccgccg
      901 gatcaacctt ggcacaccgt tttctaccag cacttcagca atcagcgggc gcgtgttgta
      961 gttcgatgac atcgaagcgc cataggcacc ggtgtcgtgc agcaccagca agtcaccaac
     1021 gcgtgccagg ggcagtgctc gggtcagcac cacgccgccc tcaccttgcg tgaagacgtc
     1081 gccggattca cacagcgggc cgcccaccac cgtggcttgc agcggaccgt caaccacggt
     1141 gccatcggca tggatcaatg ccatgccatg aaagctgcca tacatgctcg gacgcatcaa
     1201 gtcgttaaag ccggcatcaa ccatcacaaa ggcattggaa cccatttgtt tggtggcacg
     1261 cacctcggtc aacagcaccc cactgtctgc caccagatag cggcccggct cgatttccag
     1321 cttcaccggg tgacccagca ggtcggcgat ttgccttctg gcggcatccc acaattgaaa
     1381 atagtgccgg gtgtcgacgg ttggctcatc tgcttggtac ggtaccgaca gaccgccgcc
     1441 ggtcgatacg gccaccaggt ccatgccctg ctcatgaacc gtggtcacca gagccaccat
     1501 ggcaccgcag acctgctcca aatggctgta atccactcct gacccgatgt gcatgtgcaa
     1561 tccgaccagg gtcagcccgt tggcacgcaa gcgggcgcag gccagcggca gatcggtgtg
     1621 ccagatgccg tgtttgctgt gttcgccgcc agtgttggtc ttgttgctgt ggccatggcc
     1681 aaaaccgggg ttgatgcgca gccacaccgg gtggccgggg ctgacggctc ccaactggtc
     1741 aagcatgtcg atcgagccac agttgaccgg aatgcccagg ctggccacgc ggggcagggt
     1801 ggcacggtcc agcaaatcgg ccgtgaacac aatttctgca tggccgttgt gcacaccggg
     1861 ccggtagcct gccaccagcg cgcgttcaat ctcgcccaaa gataccgcgt caaccatcac
     1921 acccaggtgc cgcatctggc gcaggatatg cgtgttggag ttggcctttt gggcaaaacg
     1981 gatcacatca aactggcgca aggcgccaat ctggtgctcg atggtggccg cgtcatagac
     2041 ccacagcggg gtgccatggg tttgcgccag actggccagg gtgttgggtt ggaatgggtt
     2101 catgggtcaa tcatgccggg ttgcggcggt tcaatctatg ccagtttatt gttgtttggg
     2161 ttgcacccat gatggacatc agcggatgaa tcccacgtat gtgttcgtct gccttctggc
     2221 tcaaacacga ctgaagatga cagcgccata agcggacaaa ttgccgcagt aacgggtctc
     2281 cgggtcatcc aggtcggcat tcaggatcaa ctgccagccc tcgggcacga caaacttctg
     2341 cggctgcggc gagaagttca aaaccgtcag cgtctgcggc gcttccaggg tgatgacacc
     2401 ggtgctgcgc tcgcctgaca ccttgactga ggacatgtcg cgcggcccca gcgcgccgct
     2461 gcgtttgagg gcgatcagcg cgcggtaatg atgccagatg gcctggccct gggctgacat
     2521 gcgccgcgtc cattgcaact tggatgcctc aaaactgctg cggacatggg cctctggcgg
     2581 tgaactgctg ccaaacgcgc agtcacgctt gcggcccttt ctgaccgctt caatcaggcg
     2641 cggatcctga aaatcaacaa aaaacagaaa cggtgcggtt tcgccatatt cctcacccat
     2701 gaacagcatc ggcacaaacg gactggcaaa aaccgccgtg atgcccagca gcgcacgcga
     2761 gcggccataa cttgccatca tgcggtcacc ctgcggacgg ttgccaacct ggtcgtgatt
     2821 ttgcatgtgc accacatgct gctgggcctg ggtcagacgg ccatcggtgc ccatcatggt
     2881 gttgtgaaca cgctccagac gactgccatc aaacacaaag ccctgttgca gggctttgac
     2941 aacgtcgtca aagctgccga aatcgccata gtgccgcctg gtttcaccgg tcaggagggc
     3001 aaacaccgca tggttgaggt catcgctcca ctgagactgg tagttgaaac catccgggtg
     3061 cgtgacaaaa cggttgttgc gcaggtgctc ggcaatcatc aggatctcac gcccctcttg
     3121 ccgggcaatg cgctgcgcca gatcggtgaa ctcacgcagg atgtgcaccg ggctgttgtc
     3181 aaaaaccagc gacacggcat ccatgcgcaa accatcgaaa ccgacttgct gcagccaata
     3241 ctggatattg gccagaaaaa attcccgaat gccgtagttg tagctggcat cgaaattgat
     3301 cgcagcgccc cagggcgttg cggcgggttt ggtgtagggt gccagcgcgc cggtgtaatt
     3361 gccttccggc ccaaaatggt tatacaccac gtccagaatc accgccatgc ccagcgcatg
     3421 cgcagactcg atcaaggcct tcagatcagc gtagtcgcca tatgcctggt gcagggcaaa
     3481 ctgcgccaca ccgtcataac cccagttgcg ctcacccgga aaagccgcca acggcagcaa
     3541 ctcaatgacc gtgacaccca gggccgccag atcagcgagt ttggcggtgg cagcggccag
     3601 cgtaccctca ggggtaaaac agccgacgtg caattcgtaa atgatggcat ctgcaatgtt
     3661 gaccccagcc caggccgagg cgttg
//
LOCUS       DAOSLL010000503         3672 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_22153_length_3672_cov_5.444567, whole genome shotgun sequence.
ACCESSION   DAOSLL010000503 DAOSLL010000000
VERSION     DAOSLL010000503.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22153_length_3672_cov_5.444567"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(516..818)
                     /locus_tag="PLB25_17800"
     CDS             complement(516..818)
                     /locus_tag="PLB25_17800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43462.1"
                     /translation="MDPFQALRCDEVMYVVEEASVRLGSAAGAAQKETHLAPVNGTMP
                     QYLAWLQKRFQAHGGENFADQRQTVADLKVLVDVSALNSGWPDDVPADLVQTVVRC"
     gene            1090..1188
                     /locus_tag="PLB25_17805"
                     /pseudo
     CDS             1090..1188
                     /locus_tag="PLB25_17805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856022.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
     gene            1265..2218
                     /locus_tag="PLB25_17810"
     CDS             1265..2218
                     /locus_tag="PLB25_17810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43463.1"
                     /translation="MTREPGKFVRMIGISQASVANHHKPPKQDHHTSDDASQLLQLTA
                     HLYTARGIAADWPQLLQACRDGFQCAGGLDQLPQGAGIGPDELAALAGRISHCANFGD
                     VCGNYHQGDDQALNIKRRRCSALALHLHAAALAARKSLQAGLFEHLPPTWVLDRSGRQ
                     REANTSARELTRAGERFELRDGLLVLADPGSSRLLHKALLDVSQNTRFLWQERDDLET
                     SLLLRPLPDGQHIAVTLVPDAPSVAELASLLARNLGLTPRQSDLAAYLVTDHTLAGAA
                     RAMGISRPTANEHLAALSRQVGATDRKSLLALLRHSVNQSG"
     gene            2592..3545
                     /locus_tag="PLB25_17815"
     CDS             2592..3545
                     /locus_tag="PLB25_17815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426282.1"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOE43464.1"
                     /translation="MSEAGESVKRPVFQVDSSLGKNLSIHINRKSSMKLSSVALPGIA
                     YSGDRHQGCAAGKSYARSLPKLISAVALAVVGLAWQAPSQAALYTNNFGTNACTPSDC
                     DDCSSGQWNFSGANQNINFFGNTYSGFYVSSNGYLSFGSGSGYQGNVPIDTQTNAPMI
                     AGSFTDLFSHNDSASNVYIDNSTAGQLIVTWQEMGHFNANYSVRSTFQIVVRSDQFAI
                     GAGEGQIGFFYDTMTDPASSSAGFGDGLAASNPGEVAFHSVQPGTALNNNAPRWYNLD
                     NRPTPGGPTAVPEPGTLALTGLALLGLVGLRRRRGSDQVDK"
BASE COUNT          767 a         1076 c         1076 g          753 t
ORIGIN      
        1 taccggggtg gcgctgcgac cggtgctggc gcgctagcag gtcggcggct cggctcaggc
       61 cgtgaccgga ccaattgatg gcagattgaa ccttgatttc tcagttggac gcgcccgagt
      121 gggccgctgg cggcgcgtct gggtagggcg aaagccgcgg ctccaaacct gcccgcgcag
      181 gtttggacgg cttgagcagg ccatgcctct ggcctggctg cgccctgcaa gggagacgac
      241 gcagcacagc tcctgcccgc cagggggagc aacgacccca ggcgtgttgc cagtggctca
      301 atcgggtgcg tagcgctgtg acccaatggg tgaactccat cgaagctgaa aaagtcaata
      361 tccatgagaa ctcacggata agctcaagcg gtcaactgaa tctaggttga atggggtgaa
      421 gccagttcca cgctgactgg gcgacaaatt tggcgcaata ggccagcact gccgagtctt
      481 gggcgatgcg ctgcaggtgc gctacaggta cgcattcagc agcggaccac tgtttgcacc
      541 aggtcggcgg gcacatcgtc cggccagcct gagttcaagg cgctcacatc gacaagcacc
      601 ttcaggtctg ccacggtctg gcgctgatcg gcaaagtttt cgccgccatg cgcttggaac
      661 cgcttttgca gccaggccag gtactgtggc atggtgccgt tcaccggcgc caggtgcgtt
      721 tctttctgcg cggcaccggc ggccgacccg agcctgacgc tggcttcttc caccacatac
      781 atcacctcgt cgcaccgcag tgcctgaaac gggtccaccg ggtacagctt ggtgctgatg
      841 aaaacttatt ccctttccaa atcatccatg tcgttgttat ctcgccttgc cttgcgtttg
      901 caatgtgtgc ggcagggtgc ctagacacga atgatctaga aatggaatta cagacggaaa
      961 cacgcggtca actgaggaat ccgggtgcag gctgggtgac ttgcgtcaca gacaggatgt
     1021 gccccgagcg ggtgtaattt gcctgtcctt accttatctt ggttgtttca cttttctcaa
     1081 ggagtttgca tgaaatcgaa tgttggcggt actgaccgca ttgtgcgcat tgttctgggc
     1141 atggcactga ttggccggac actcactggc agcatcggtg tctggggcaa gtgaactcaa
     1201 gagaaaaatg atactcatga ttgaaagtcc ctcaacacac cgttggaaaa ttggaaacgc
     1261 gtcgatgacc agagagccag gcaaatttgt tcggatgatt ggcatcagcc aggcatcggt
     1321 ggccaaccac cacaaacccc ccaaacaaga tcaccacacc agcgacgatg ccagtcagtt
     1381 gctgcagcta acagcgcatc tgtacaccgc gcggggcatt gccgccgatt ggcctcaact
     1441 gctgcaagcc tgtcgcgacg gttttcaatg cgcggggggg ctggaccagt tgccccaagg
     1501 cgcgggaatc ggacccgatg aactggccgc gctggccggg cgcatcagcc actgcgccaa
     1561 ttttggcgac gtgtgtggca actatcatca gggtgacgac caggcgctca acatcaagcg
     1621 tcggcgctgc tctgcgctgg cgctgcacct gcacgccgcc gccctggcgg cccgcaagtc
     1681 gctgcaggcc ggcttgtttg aacacttgcc gcccacttgg gtgctcgacc gaagcgggcg
     1741 acagcgtgag gccaatacca gcgcgcgcga actgacccgt gcgggtgagc gctttgagct
     1801 cagggacggc ctgctggtgc tggctgaccc aggctccagc cgcttgctgc acaaggcatt
     1861 gctggatgtc agccagaaca cccgcttttt gtggcaagag cgcgacgacc ttgaaaccag
     1921 cttgctgttg cggccattgc cggacggcca gcacatcgcc gtcacgctgg tgcccgatgc
     1981 gcccagtgtg gccgaactgg catcgctgct ggcccgcaat ctgggcctga caccacgcca
     2041 gagcgacctt gccgcctacc tggtgacaga ccatacgctg gctggcgcag cccgcgccat
     2101 gggcatttcg cgccccaccg ccaacgagca cctggctgcg ctgtcacggc aggtcggcgc
     2161 aaccgatcgc aaatcgctgc tggccctgct gcggcacagc gtcaaccaat caggctgacc
     2221 aaaagccgcc gaggcctctt tgtcctggcg cgattgactg ggcagtcatg gattgcgggg
     2281 cttgcgcaca cagttgtcgg atttttttac agttgcaagc caggagcgtc aaaaccggac
     2341 cccgacactg tgtctggaac aagccacgca actcacagca ataaccgggt ttcaagaggt
     2401 catcgtgaaa aatttgcgac ttttcggccg ggaagttgtc attcccgagc agccgtatcc
     2461 tgacaaagtg cagggtaaaa ggcagttgac agaaagatct cagttgcgct ggcgtaggcg
     2521 aaaattatta tataaatcaa tagcttacag aattatttgc agttggcaca gattgtgcct
     2581 tttaacccag gatgagcgaa gctggagaaa gcgtcaagag accggttttt caggtcgact
     2641 cttctcttgg gaaaaattta tctatccata tcaacagaaa gtcatcaatg aaactaagtt
     2701 ctgtcgcact gccaggcatt gcgtattccg gtgatcgtca ccaggggtgc gcggcgggca
     2761 aaagttacgc ccgatccctg ccaaaactaa tctccgctgt tgcgcttgcc gtggtgggcc
     2821 tcgcctggca agccccgagc caagcggcgt tgtacaccaa taattttggc accaacgctt
     2881 gcacccctag cgattgcgat gactgctcat caggtcagtg gaatttctcc ggggccaacc
     2941 agaacatcaa ctttttcggc aacacctatt cgggcttcta tgtgagctcg aatggttacc
     3001 tgtccttcgg cagtggctcg ggataccagg gtaacgttcc gattgatact cagactaatg
     3061 cgccgatgat cgcgggttcc tttaccgatc tattttcaca caatgacagc gcgtcgaacg
     3121 tgtacatcga caactccact gccggacagt tgattgtcac ctggcaagaa atgggccatt
     3181 tcaacgctaa ctacagcgtg cgcagcacct tccagatcgt cgttcgttcc gaccagttcg
     3241 cgatcggtgc cggtgaggga caaatcggtt tcttttacga cacgatgacc gatcccgcca
     3301 gcagcagcgc agggtttggc gatggtttgg cagcaagcaa tccgggcgaa gtggcgttcc
     3361 attcggtgca gcccggcacg gctctcaaca acaatgcgcc gcgctggtac aaccttgaca
     3421 accgtcctac gcccggtggt ccgaccgcgg ttccggaacc cggaaccctg gcgttgaccg
     3481 ggctggcgct tctggggctg gtcgggctga ggcggcgcag gggatcggat caagtggata
     3541 aatgagatta aaggggccag gctcggattg ctctctccgc gaacgcagtg atattggggc
     3601 cggatcccga ttcggcaccc agccgcatgt tcgccaccac tgcctcaccg aatctggctc
     3661 tgaccccaat ag
//
LOCUS       DAOSLL010000504         3645 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_22348_length_3645_cov_4.827298, whole genome shotgun sequence.
ACCESSION   DAOSLL010000504 DAOSLL010000000
VERSION     DAOSLL010000504.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22348_length_3645_cov_4.827298"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            448..1776
                     /gene="xseA"
                     /locus_tag="PLB25_17820"
     CDS             448..1776
                     /gene="xseA"
                     /locus_tag="PLB25_17820"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HOE43465.1"
                     /translation="MPEPRVWQVGALCRAVADALQARFNPVQVRGELSGFSRAASGHC
                     YFSLKDANGQLRCAMFKRAASDLGFSPRDGELVEVAGRLGVYEPRGDLQLIVERMSRS
                     GVGNLFEQFQKILLKLQGEGLFDASRKRALPLLPRAIGLVTSLGAAALHDVASALQRR
                     VPHIPVLLVPAAVQGNNAPAELISALLKLYHLTQTGQGLDGNYVQKKGSAAGAWPLTV
                     DVIVLVRGGGAMEDLWAFNDEQLARTLAQSPVPVVCGVGHETDFTIADFVADLRAPTP
                     TAAAELVAQPRLAWLDLLDGLQRRLQQALYRQLDRHSQRLDRLASNLGRPSGRVATQA
                     QRLARFAQTLQVVTRHAMQQQQQRLDRLAAQLPLSLQRGLLVQQQRLDRAALRLGLLD
                     PHLVLERGYAWLSDAQGAAVTSCQQLVVGQAIKATLFDGTVDLTVANPPG"
     gene            1822..2403
                     /locus_tag="PLB25_17825"
     CDS             1822..2403
                     /locus_tag="PLB25_17825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HOE43466.1"
                     /translation="MEHTLPALPFAIDAMAPHYSKETLEFHHGKHHNAYVVNLNNLQK
                     GTEFETMDLESIIKKASAGIYNNAAQIWNHTFFWNCMTPAGGGEPAGALAAAITAKWG
                     SYAAFREAFVKSAVGNFGSGWTWLVKKADGTVDIVNMGAAGTPLTTADKALLTVDVWE
                     HAYYIDYRNLRQKFVETFLDKMVNWSFVQSNFA"
     gene            2648..>3645
                     /locus_tag="PLB25_17830"
     CDS             2648..>3645
                     /locus_tag="PLB25_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE43467.1"
                     /translation="MTSYADFHQRSLTDRDGFWAEQAQLVDWKVQPKQICDYSNPPFA
                     KWFVGGVTNLCHNAVDRHLKDRADQAALIYVSTETNVEKVYSFRELHAEVQRMAAALK
                     DLGVQKGDRVLIYMPMIAEAAFAMLACTRIGAIHSVVFGGFASGSLASRIEDAEPRVI
                     VSSDAGSRGGKPVAYKPLLDEAIRLSTHKPEAVLMIDRGMVAMDLVAGRDHLWGALRE
                     KNLNTVVECEWVESTHSSYTLYTSGTTGKPKGVQRDTGGYCVALAASMKHIYAGNAGE
                     TYFSTSDIGWVVGHSYIIYGPLIAGMATILYEGLPTRPDGGIWWSLVEKYKVTVMF"
BASE COUNT          757 a         1017 c         1111 g          760 t
ORIGIN      
        1 gcaggcaatc agcggccaga tcggccagcc tgcggcttgt atgatggaaa acaaattggg
       61 gctccgctca tgaaaaatgc tgtgcgatta tgacgcagcg ccagatcggc ttgacggtgt
      121 cgtgctgggc gtgtgtgcgg cttcgggccg gggtcagaag ggccttcaag cacccggtct
      181 gcgcacaatt tctgtggata actttgtgga gaaaccccgg tggaactggc tgcaggccgc
      241 gtcaaacgtg gcttgtggtg gtgtgatgat aaattgggca gcaaaaaatt caatgaaatc
      301 aatgattttt aacgaacatg caggaaaccg cgctacgcaa tttgggatgc tggtgattgc
      361 gcaaaattgt ggacgacttc aacttgcaag atcatttcaa acacacattt tcaagagggt
      421 tttcaggtgt ctgatgccgc tcaattgatg cccgagccgc gcgtctggca ggtcggcgcg
      481 ctctgccggg cggtggccga tgcgctgcag gcccgattca acccggttca ggtgcgaggt
      541 gagttgtcgg ggttttcccg agcggccagc ggtcactgct atttctcgct caaggatgca
      601 aacgggcaac tgcgctgtgc catgttcaag cgtgccgcct cggacctggg ttttagtccg
      661 cgtgacggcg aactggtgga ggtggctggt cgcctgggcg tgtatgagcc gcgcggtgac
      721 ctgcaactga ttgtggagcg catgagccgc tcgggagtcg gcaatttgtt tgagcagttt
      781 cagaaaatct tgctcaagct gcaaggcgag ggcctgtttg atgccagccg caaacgtgcc
      841 ctgccactgc tgccacgcgc catcgggctg gtgacctcgc tgggcgctgc agccttgcac
      901 gatgtggcaa gcgcgctgca gcgacgtgtg ccgcacattc cggtgctgct ggtgccggcc
      961 gcagtgcaag gcaacaatgc gccagccgag cttatcagtg cgctattaaa gttgtatcat
     1021 cttacgcaga caggacaagg gctggatggc aattatgttc aaaaaaaagg gtcggcggca
     1081 ggggcctggc cgctgacggt ggacgtgatt gtgctggtgc gtggcggcgg tgccatggag
     1141 gatttgtggg cctttaacga tgaacaactg gcgcgcaccc tggcgcaaag cccggtgccg
     1201 gtggtgtgcg gcgtgggcca tgagaccgac ttcaccatcg ccgattttgt cgccgacctg
     1261 cgggcgccaa ccccgactgc cgccgccgaa ttggtcgccc aaccgcgctt ggcctggctg
     1321 gacttgctcg atgggctgca acggcgcttg caacaggcgc tgtatcgcca gcttgaccgc
     1381 cacagccagc gactggaccg actggccagc aatctggggc ggccttctgg ccgagtggcg
     1441 acgcaggcgc agcggctggc ccgcttcgcc cagacgctgc aagtggtgac gcgccatgcg
     1501 atgcagcaac agcagcagcg ccttgaccgg ctggcggctc agttgccgct gtcgctgcag
     1561 cgtggcctct tggtgcagca gcagcggctg gaccgtgccg cactgcgcct cggtctgctt
     1621 gacccgcatc tggtactgga acgcggctac gcctggctta gtgacgcgca gggtgcagca
     1681 gtgacaagtt gtcagcagct tgttgtcggc caggccatca aggccactct gtttgacggc
     1741 acggttgatt tgaccgtggc caacccgccg ggctgaaagt tcctacaatt tcacgctttc
     1801 gcaacccacc cagaggaatc catggaacat accctgcccg cactgccatt tgccattgac
     1861 gcgatggcac cccactattc caaagaaacg ctggagtttc accatggcaa acaccacaac
     1921 gcctatgtgg tgaacctgaa caacctgcaa aaaggcactg agttcgagac catggacctt
     1981 gaatcgatca tcaaaaaggc cagcgcaggc atctacaaca acgccgcaca aatctggaac
     2041 cacacttttt tctggaactg catgacacct gccggtggcg gcgagcccgc tggcgcgctg
     2101 gctgctgcca tcactgccaa gtggggcagt tacgcggctt tcagggaagc ttttgtcaag
     2161 tcagccgtgg gcaactttgg ttcaggctgg acctggctgg tcaagaaggc cgacggcacg
     2221 gttgatatcg tcaacatggg tgcggctggc acgccgctga ccaccgctga caaggccctg
     2281 ctgacggttg atgtgtggga acatgcctac tacattgact accgcaacct gcgccagaag
     2341 tttgtcgaaa catttctcga caaaatggtc aactggtcgt ttgtgcaaag caactttgcc
     2401 tgagccacaa gcgatttttt gccacgcttt gccgttgcgc cgattcccgt cggttgaaac
     2461 accgcaacgg taaacaaccc aggcatgatc agcaaccgcc agtgccatat ggcacggtga
     2521 gttgctgcgc agactcaagt tggcggcgag attgggcgct gtgcaacttg cccagaaaag
     2581 tcaggaaact gcaagctgac tgttcataat tgcgattcct ttttttaatg acgatcggag
     2641 acattcaatg accagttacg ccgacttcca ccaacgttcc ctgaccgatc gagacggttt
     2701 ttgggccgag caagcccagt tggtagactg gaaagtgcaa ccgaaacaaa tttgcgacta
     2761 cagcaaccca ccgtttgcca agtggtttgt cggcggtgtt accaatttgt gccacaacgc
     2821 ggtggatcgt cacctgaaag accgggctga tcaggcagcg ctgatttatg tatcgaccga
     2881 aaccaatgtt gaaaaggttt attcgtttcg cgaactgcac gcagaagtgc aacgcatggc
     2941 agcagcgctg aaggacctgg gggtgcaaaa aggtgaccgg gtgctgattt acatgccgat
     3001 gattgccgag gccgcttttg ccatgctggc ttgcacccgc attggcgcca ttcactcggt
     3061 ggtgtttggc ggctttgcat cgggctcgct ggcctcgcgc attgaagacg cagaacctcg
     3121 cgtcatcgtc agttccgatg ccggttcacg cggtggcaag ccggtggcct acaagccgct
     3181 gcttgacgaa gcgattcgcc tgtcgactca caagccagag gcagtgctga tgattgatcg
     3241 gggcatggtg gccatggacc tggtggcagg ccgtgaccat ctttggggtg cgttgcgcga
     3301 aaagaacctc aacacggttg tggaatgtga atgggtcgag tccacccact ccagctacac
     3361 gctctacacc agcggcacaa ccggcaaacc caaaggcgtt caacgtgaca ccggcggcta
     3421 ctgtgtggcg ctggccgcca gcatgaagca catctacgct ggcaacgctg gtgaaaccta
     3481 tttctcgacc agcgacatcg gctgggtggt cgggcacagc tacatcattt atggcccgct
     3541 gattgctggc atggcaacca ttctgtacga aggcctgccg acccgtcctg acggcggcat
     3601 ctggtggagc ctggtggaaa aatacaaggt cacggtcatg ttcag
//
LOCUS       DAOSLL010000505         3641 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_22378_length_3641_cov_4.226157, whole genome shotgun sequence.
ACCESSION   DAOSLL010000505 DAOSLL010000000
VERSION     DAOSLL010000505.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22378_length_3641_cov_4.226157"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="PLB25_17835"
     CDS             complement(<1..649)
                     /locus_tag="PLB25_17835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HOE43468.1"
                     /translation="MGLLAIAAVIPSLAQEPKPVKLNTTADHTKFKELQKEFATGPEV
                     TKVCLSCHTEAAGQIHRTKHWKWEYTNPDTQQVLGKKHVINNYCIAIASNEASCNSCH
                     IGYGWKDASFDFKSEENVDCVVCHDTTGNYKKPSGMAGNVVTKDTEFPPGSGKIIKGI
                     DLTKIAQKVGKSSRDTCGACHFNGGGGDGVKHGDLDSSLAAPEKELDVHMDASGLD"
     gene            1143..>1988
                     /locus_tag="PLB25_17840"
     CDS             1143..>1988
                     /locus_tag="PLB25_17840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase small subunit"
                     /protein_id="HOE43469.1"
                     /translation="MLLSLKGVTPPAILALADGTVFIGNSIGSTGSTVGEVVFNTAMT
                     GYQEILTDPSYCRQIVTLTYPHIGNYGISVQDIEADKIHAAGLIIKDLPLLASNFRAT
                     MSLHEYLLSEGTVAIANIDTRQLTRQLRSLGAQNGCILALTDGESVTPAAIDRALLLA
                     KAAPSMAGTDLAKVVSAKAAYDWTQTEWALFNASLGGKPGYGQQTDADYSVVAYDFGV
                     KKNILRMLAQRGCKVTVVPAQTTAAEVLKFRPDGIFLSNGPGDPQPCDYAIAAAAELI
                     NTGIPT"
     assembly_gap    1989..1998
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1999..2340
                     /locus_tag="PLB25_17845"
     CDS             <1999..2340
                     /locus_tag="PLB25_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase small subunit"
                     /protein_id="HOE43470.1"
                     /translation="FGICLGHQIMALASGAKTFKMKFGHHGANHPVKDLDSGRVSITS
                     QNHGFAVDESTLPANLRATHISLFDGTLQGLERTDQPAFCFQGHPEASPGPHDIGYLF
                     DRFIASMESRK"
     gene            2337..3014
                     /gene="leuE"
                     /locus_tag="PLB25_17850"
     CDS             2337..3014
                     /gene="leuE"
                     /locus_tag="PLB25_17850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HOE43471.1"
                     /translation="MSFYGVTDVWTYVVGAFGIILLPGPNSLYVLSVATARGVRAGYH
                     GAFGVFLGDSILLLCTALGAASLLRTHPALFLVVKYAGAGYLCWVGLNLLRSTLSGLR
                     ARADALDAVAAAAAPGILANANLQRPFRRALVISLLNPKAILFLLSFFVQFIDPGYAH
                     PELPFLILSAILMIFSAVYLTALIFAGARLANTFNQRKRLSASLSSLVGGLFVWFGAK
                     LATANLN"
     gene            3162..>3641
                     /gene="carB"
                     /locus_tag="PLB25_17855"
     CDS             3162..>3641
                     /gene="carB"
                     /locus_tag="PLB25_17855"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836975.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HOE43472.1"
                     /translation="MPKRTDIQSVLIIGAGPIIIGQACEFDYSGVQACKALREEGYKV
                     ILINSNPATIMTDPATADVTYIEPITWKTVEKIIAKERPDAILPTMGGQTALNCALDL
                     WHNGVLSKYTGAATGKPVELIGATPQAIDKAEDRLKFKDAMTRIGLGSAKSGIAHSMD
                     "
BASE COUNT          732 a         1020 c         1002 g          877 t
ORIGIN      
        1 agtccagacc tgaggcatcc atgtggacat caagctcttt ttcgggcgcg gccagcgaac
       61 tgtccagatc gccatgcttg acaccatcgc cgccgccgcc gttgaaatgg caggcaccac
      121 aagtatccct gctggacttg ccaactttct gggcaatttt tgtcaggtca atgcctttga
      181 tgatcttgcc ggacccaggt gggaactcgg tgtccttggt gaccacgtta ccggccatgc
      241 cagatggctt tttgtagttg ccggtggtgt cgtggcacac cacgcagtcc acgttttcct
      301 ccgacttgaa gtcgaacgag gcatctttcc atccatagcc aatatgacaa ctgttgcagg
      361 atgcttcgtt ggaagctatt gcaatgcagt aattgttgat aacgtgtttt ttgcccaaga
      421 cctgctgggt gtcggggttg gtgtattccc acttccagtg ctttgtgcgg tggatttgtc
      481 cagcggcttc ggtgtggcaa gaaagacaga ccttggtgac ctctggaccg gttgcgaact
      541 ctttttgcag ttccttgaac ttggtgtggt cggcggtggt gttcagcttg acgggctttg
      601 gttcttgtgc aagcgatgga atgaccgcag caattgctaa caatcccacc agaatgagct
      661 ttgatattgc aactttcgac ttgattttca tacgagtttt agcgtctatt ctgaatattc
      721 gaaactacta aattgaattg ttgcgccaaa tcagagcatc ttattgattc aaatcaataa
      781 aagtggcgaa attgattgat atttcgcaag ctaatgtcgc ttcaatctgt ctaatcctgg
      841 ttgtttggca gacccacttg cgtttcgaaa tgttccgatt tccagagacg tggcattttc
      901 gatttcgcgc tggcacgacg cggtggtgaa cctcgatcac caaaacaagt ggcgcagagt
      961 gatggggtta gcccctgctg gtctggcacg ttgaaggctt tttgcaggcc cctcggctat
     1021 aatcaagggc tatttcgcac ctgcggacgc tctcagcatc ttgccccatc cccaccaaat
     1081 tcatccaaga gaaccctctt gagcgtgcct gggcgtaccc gcaaatcatt tcggagaacc
     1141 cagtgctttt gtccctcaaa ggggttaccc ctcccgccat tctggcactc gcagatggca
     1201 cggtctttat cgggaattcc atcggttcca ccggctcaac cgttggcgaa gtggtgttta
     1261 acactgccat gaccggttac caggaaattc tgaccgaccc cagttactgc cgccagatcg
     1321 tcacgctcac ttatccgcac attggcaact acggcatcag tgtgcaagac attgaggccg
     1381 acaagattca cgcggcaggt ctgatcatca aggatttgcc gttgctggca tccaactttc
     1441 gcgccaccat gagcctgcac gaatacctgt tgtctgaggg cacggttgcc atcgccaaca
     1501 tcgacacccg gcagctcacc cgtcaactgc gttctctggg ggcgcaaaac ggctgcatcc
     1561 tggccttgac cgatggcgaa agcgtcactc cggcggcaat tgaccgcgcg ctgctgctgg
     1621 ccaaggcagc gccgagcatg gcaggcaccg accttgccaa agtggtgtcg gccaaggcgg
     1681 cttatgactg gacgcagacc gaatgggctt tgttcaacgc ctcgctgggt ggcaagccgg
     1741 gttatggcca gcagactgac gcggactact cggtcgtagc ctacgatttt ggggtgaaaa
     1801 agaacatttt gcgcatgttg gcccagcgtg ggtgcaaggt gacggtggtg ccggcacaaa
     1861 ccactgctgc cgaggtgctg aagttccggc cagacggcat atttttgagc aacggcccag
     1921 gcgacccgca gccttgcgac tacgccatag ccgctgcagc agaactgatc aacaccggca
     1981 tacccactnn nnnnnnnntt tggtatttgt ctggggcacc agatcatggc gctggccagc
     2041 ggtgccaaaa ccttcaagat gaagtttggc caccacggtg ccaatcaccc ggtcaaggac
     2101 ctcgacagtg gacgcgtcag catcaccagc cagaaccacg gttttgcggt tgatgaaagc
     2161 accctgcctg ccaatttgcg tgccacccac atcagcctgt ttgatggcac gctgcaggga
     2221 cttgagcgca ccgatcagcc agccttctgt tttcaggggc acccggaagc ttcgcccggt
     2281 ccgcacgaca ttggctattt gtttgaccgc ttcatcgctt cgatggagtc gcgcaaatga
     2341 gtttttacgg tgtgaccgat gtgtggactt acgtcgtcgg tgcgtttggc atcattttgt
     2401 tgcccgggcc caactcgctg tatgtgctgt cggtggccac cgcacgcggc gtgcgggctg
     2461 gctaccacgg agcgttcggg gtgttcttgg gcgacagcat tttgctgctg tgcaccgctt
     2521 tgggtgccgc cagcttgctg cgcacccatc cggcactgtt cctggtggtc aagtatgcgg
     2581 gggcaggcta tctgtgttgg gttggtctga acttgttgcg ttcaaccctg tcgggtctgc
     2641 gagcacgcgc tgatgccttg gatgcggttg ccgcagcggc ggccccgggc atcttggcaa
     2701 atgcgaattt gcagcgacct tttcgccgtg ccttggtcat cagcctgctg aacccgaagg
     2761 cgattttgtt tttgctgtcg ttttttgtgc agttcattga tccgggctat gcccatcctg
     2821 agctgccgtt cctgattctg agcgctattt tgatgatctt cagtgccgtt tacctgacgg
     2881 cgctgatttt tgccggtgcc cgcctggcaa acaccttcaa ccagcgcaag cgcttgtcgg
     2941 ccagcttatc gagtctggtg ggaggcctgt ttgtctggtt cggtgccaag ctggcgaccg
     3001 ccaacttgaa ctgatgtgga tgggcagcgt catctattcg ctgtcttgtc atgccagcac
     3061 ggcagccaat gtcacaggcc atcctgacct tttttgaacc tgaatttgca gttgcagccg
     3121 ccagcggctg cgtgtacccc accaaatagc agtctgacac tatgccaaaa cgtactgata
     3181 tccaaagcgt cctcatcatc ggtgctgggc ccatcatcat cggacaagcc tgcgagtttg
     3241 actattccgg cgtgcaggcc tgcaaggcgc tgcgcgaaga gggctacaag gtcatcctga
     3301 tcaacagcaa cccagccacc atcatgaccg acccggcaac ggccgatgtg acctacattg
     3361 agcccatcac ctggaaaacg gtcgaaaaaa tcattgccaa agagcgcccg gatgcgattt
     3421 tgccaaccat gggtggccaa accgcgctga actgtgcgct ggacctgtgg cacaacggcg
     3481 tgttgagcaa atacaccggt gccgcgactg gcaagccggt cgaattgatt ggcgcgacac
     3541 cgcaagccat cgacaaggcc gaagatcgcc tgaaattcaa ggatgccatg accaggattg
     3601 gcctgggatc agccaaatcc ggcattgccc acagcatgga t
//
LOCUS       DAOSLL010000506         3635 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_22429_length_3635_cov_3.671508, whole genome shotgun sequence.
ACCESSION   DAOSLL010000506 DAOSLL010000000
VERSION     DAOSLL010000506.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22429_length_3635_cov_3.671508"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(119..931)
                     /locus_tag="PLB25_17860"
     CDS             complement(119..931)
                     /locus_tag="PLB25_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF547 domain-containing protein"
                     /protein_id="HOE43473.1"
                     /translation="MLLRRNFIALLALPVFASQTQAQAQAVPTSDWDALLKKHVRSLR
                     GSQATEVDYAGFAADRTRLQSWLAGNGKVTRAEFDRWGKPEQLAFLINAYNAWTVELV
                     LTGYPGVQSIKDLGSFVQSPWKKSFVPLLGETRSLDDIEHGLIRGSARYNEPRIHFAV
                     NCASIGCPALRAQAYNAAQLEAQLEDATRQFLSDRTRNRFESGALKVSSIFKWYRKDF
                     EQGWRNARSLGQFLALYSSALGLSAEVASQVASGQVNIEFLDYDWRLNGLRS"
     gene            complement(934..3105)
                     /locus_tag="PLB25_17865"
     CDS             complement(934..3105)
                     /locus_tag="PLB25_17865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE43474.1"
                     /translation="MKQILILVAAAVLILGFFALDLNQYLTLEGMKASLGQFEAHRAA
                     APVVVGLAFFGVYVAVTALSLPGAAILTLAAGALFGLTVGTLIVSFASSLGATLAFLA
                     SRYVLRDAIQRRFGERLKAINEGMAKDGPLYLFTLRLVPVFPFFLVNLLMGLTPIRTL
                     SYYWVSQVGMLAGTLVYVNAGTQLAQITSLSGIVSPGLLLSFALLGVFPMLAKKFLGF
                     LQSRRVYAKWLRPKSFDRNLVVIGGGAAGLVSAYIAAAVKAKVTLVEAHKMGGDCLNY
                     GCVPSKALIKSARIANQMRHAEDYGLSATEPRFSFSKVMARVHAVIAAVAPHDSVARY
                     TELGVEVLQGYAKIVGPWTVEIKLNDGGTQRLTTRAIVIAAGARPFVPPLSGLDDVGY
                     VTSDTVWDEFARLDAPPARLVVLGGGPIGCELAQSFARLGSRVTQIEMAPRIMIREDI
                     EVSDLARDALARDGVDVLTGHKALRCEREGIEGVGDGVRKFIVVEHQGQEERIEFDAL
                     LCAVGRVARLQGYGLEDLGIPTQRTVAVNDYLETLYPNIYAAGDVAGPYQFTHTAAHM
                     AWYAAVNALFGDFRKFKVDYSVVPWATFIEPEVARVGLNEQDAKEKNIPYEVTKYGID
                     DLDRAIADGTAHGFVKVLTVPGKDRILGVTIVGEHAGDLLAEFVLAMKHGLGLNKILG
                     TIHTYPTLAEANKYAAGEWKRAHAPQKLLALLGRFHAWRRG"
BASE COUNT          832 a         1105 c          996 g          702 t
ORIGIN      
        1 cacggtgcac cagtttgtgg cgcagagggg tctggggtat gcgcagatga acccggtttg
       61 atacaaaaac catagctgct agcgcttatt ctgcaagggc tggaggccaa aaactctgtt
      121 acgaccggag cccgttgagc cgccagtcat agtccagaaa ctcaatgttg acctgtccac
      181 tggcaacttg gctggccacc tcagcgctta aacccaaagc gctgctgtac agcgccagaa
      241 actggcccag gctgcgggca ttgcgccagc cctgctcgaa atccttgcgg taccacttga
      301 agatgctcga caccttgagc gcaccggact cgaagcggtt gcgggtgcgg tccgacagga
      361 actggcgcgt tgcgtcctcc agttgtgctt caagttgtgc cgcgttgtaa gcctgcgccc
      421 gcaatgccgg gcagccaatg ctggcgcaat tcaccgcaaa atgaatgcgc ggctcgttgt
      481 aacgagcaga accgcgaatc aggccatgct cgatgtcgtc gagcgaccgg gtttcgccca
      541 gcaacgggac aaaactcttt ttccagggcg attggacgaa agaacccagg tctttgatgg
      601 actgaacgcc ggggtagccg gtgagcacca gttccaccgt ccatgcgttg taggcattga
      661 tcaaaaacgc caactgctcg ggcttgcccc agcggtcaaa ttcggcgcgc gtcactttgc
      721 cattgccagc cagccagctt tgcagccgtg ttcgatctgc ggcaaatccg gcgtaatcca
      781 cctcggttgc ctggctcccg cgcaagctgc gcacatgttt cttcaacagc gcatcccagt
      841 cgctggtcgg cacggcttgt gcctgcgcct gcgtctgcga ggcaaaaact ggcaaggcca
      901 gcagggcaat gaaatttcgg cgtaacaaca tgatcaacct ctcctccagg catgaaatcg
      961 ccccaacagg gccaacagct tttgcggtgc atgggcgcgc ttccactcgc ctgccgcata
     1021 tttgttggcc tcggccagcg tcgggtaggt gtgaatggtg cccagaattt tgttcaggcc
     1081 cagaccgtgc ttcatggcca acacaaattc agccagcagg tcgccagcgt gttcgcccac
     1141 aatggtgaca cccagaatgc ggtctttgcc gggcacggtc agcactttga caaagccgtg
     1201 cgcggtgcca tcggcaatgg cgcggtccag gtcgtcaata ccgtacttcg tgacttcata
     1261 cgggatgttt ttttctttgg catcttgctc attgagcccc acacgggcaa cttcgggctc
     1321 aataaaggtc gcccagggca cgactgaata gtccaccttg aactttctga aatcaccaaa
     1381 cagcgcattc acggctgcat accaagccat gtgtgctgcc gtgtgggtaa attgataggg
     1441 gccggccacg tcgcctgctg cgtaaatgtt ggggtaaagg gtttcaaggt aatcgttcac
     1501 cgccacggtg cgctgggtgg gaatgcccaa atcttccaga ccgtagcctt gcaggcgggc
     1561 cacccggccc acggcgcaca gcagggcatc gaactcaatg cgctcttctt gcccctgatg
     1621 ttcaaccacg atgaacttgc gtacgccgtc gcccacaccc tcaatcccct cacgttcaca
     1681 acgcaacgcc ttgtggccgg tcaacacatc cacgccatcg cgggccaaag catcgcgcgc
     1741 caggtcagaa acctcgatgt cttcgcggat catgatgcgc ggtgccatct cgatctgcgt
     1801 caccctggag cccaggcggg caaaactttg cgccagttcg cagccaattg ggccgccgcc
     1861 caacaccacc agacgtgccg gcggcgcatc caaccgggca aattcgtccc acaccgtatc
     1921 gctggtgaca tagcccacat cgtccagccc cgacagcggc ggcacaaacg ggcgggcacc
     1981 ggcggcgatg acgatggcac gggtggtcag gcgttgtgtg ccaccgtcat tgagcttgat
     2041 ttcaacggtc cagggaccga cgatttttgc gtagccttgg agcacctcca cgcccaattc
     2101 ggtgtagcgc gccacgctgt catggggtgc gacggcagca atcaccgcat gcacacgggc
     2161 catcaccttg ctgaaactga accggggttc agtcgcactc aagccatagt cttctgcatg
     2221 ccgcatttgg ttggcaatgc gggccgactt gatcaacgcc ttgctcggca cacagccata
     2281 gttcaggcaa tcgccaccca ttttgtgcgc ttcaaccaaa gtcactttgg ccttgaccgc
     2341 tgccgcaatg taagcgctga ctaggcccgc tgcaccgcca ccaatcacca ccagattgcg
     2401 gtcaaatgat ttgggccgca gccatttggc gtaaacccgg cggctttgca aaaagcccag
     2461 gaacttcttg gccagcatgg ggaacacgcc aagcagtgca aacgaaagca gcaggccggg
     2521 cgacacaatg cccgacaaac tggtgatttg ggccaattgg gtaccggcgt tgacatagac
     2581 cagggtaccg gccagcatcc ccacttggct gacccagtag tagctcaagg tgcgaatggg
     2641 ggtcaggccc atcagcagat tgaccaaaaa gaacggaaac accggcacca gacgcaaggt
     2701 gaacaggtac aacggcccgt ctttggccat gccctcgttg atggccttga gccgttcgcc
     2761 aaaacggcgc tgaatggcat cgcgcagtac ataacgggag gccagaaacg ccagggtcgc
     2821 ccccaggctg gaggcaaacg acactatcaa agtgcccacc gtcaagccaa acaaggcacc
     2881 agccgccagc gtcagaatgg cagcccccgg caacgaaagc gccgtgactg ccacgtaaac
     2941 accaaaaaat gccagcccaa cgacaaccgg cgctgccgcg cgatgcgcct cgaattgccc
     3001 caggctggct ttcattcctt ccagcgtcag gtattggttc aagtccagcg caaaaaagcc
     3061 aaggatcaga accgcagcag cgacaagaat cagtatttgt ttcatgggtt gcccatagtt
     3121 gttcaattga tgcagggcaa gaaaagctag cccggcccag acatagtcgg gtgtgctgcg
     3181 tccattctta cactgcgagc ggcttttctg gctggaattg atcgccatgc aggccatatt
     3241 cttgccaact gggaagagtg ccaccgcagt gcggaacttg ttgctctgct tgagcgactt
     3301 gatgagtaca aaaacaacct attggtgcct ttttggattc ccgccctcgc gggaatgacg
     3361 gcgctgccat gcgaacaaat tcattcggat cacccgatac ccaaatcaac ccaagctggc
     3421 caatttggcc aactactcag gcattggact tcccacaaga tggctaaggt tttgcccacg
     3481 gagccgaggc tctgggcaca acgccatgca gaagtgacaa aattgtcaac cgtcacgata
     3541 caactgacaa aaacggaagg gagaccggtg ccgtgcaact caaaatgaca caatttggtc
     3601 ttggaaagcg ccaaatacag cgataagatg cttgg
//
LOCUS       DAOSLL010000507         3634 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_22433_length_3634_cov_5.412406, whole genome shotgun sequence.
ACCESSION   DAOSLL010000507 DAOSLL010000000
VERSION     DAOSLL010000507.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3634
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22433_length_3634_cov_5.412406"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PLB25_17870"
     CDS             <1..470
                     /locus_tag="PLB25_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009665360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOE43475.1"
                     /translation="YAQWLATALDIDQQLAWLTGMMQRLGELLIGQVDPLALAEIEAL
                     PHLPGGRWEREKRLIGFSEGQITAELARRWNFPMQIVQALERVYDPLVEQAFSRMGAV
                     VNLAGLLADTPAASAETVDDLPHDVIAALGLDVRWMKATFPAAESFVNVSLAS"
     gene            467..1111
                     /gene="nth"
                     /locus_tag="PLB25_17875"
     CDS             467..1111
                     /gene="nth"
                     /locus_tag="PLB25_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HOE43476.1"
                     /translation="MKPADIDAFFSTLAASNPLPQTELAFASVFELLTAVLLSAQATD
                     VSVNKVTQQLFLLAGTPQKMLALGLEGLESCIKTIGLYHAKARHLMQTCQMLLDLHDG
                     QVPRTREALEALPGVGRKTANVVLNVAFGEPTMAVDTHIFRLANRTGLAPGTTPRAVE
                     QALLKQVPAQFQPDAHHWLILHGRYVCKARTPRCWQCSLAKYCDFKPKPTGPTS"
     gene            1108..1947
                     /locus_tag="PLB25_17880"
     CDS             1108..1947
                     /locus_tag="PLB25_17880"
                     /inference="COORDINATES: protein motif:HMM:NF020252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOE43477.1"
                     /translation="MTLTPSNAAHPDLSAFLTTLDWPVMPEVGQALLRTFADEDADID
                     TVSRIISKDPALTATLLRMANSAMFGLSGTVDTLDRAISVVGMSLIRARALSICLARL
                     VNLPAGMDRMAFWRYCMLCAGYSQWLAAQCEADVQEAWLAGMMLRLGEISLCRVRPEV
                     LPQLQAKPGEPGVRWSLQRHLLGFDEGQVVAEMARHWDFPGTLVQGLQNAAQPLLAQK
                     MSKLAAVLHLAARLADSDGVNEASLDALPLMVVLLLKLDLKALLTSAPDADVLADITM
                     FQH"
     gene            complement(2001..>3634)
                     /locus_tag="PLB25_17885"
     CDS             complement(2001..>3634)
                     /locus_tag="PLB25_17885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase"
                     /protein_id="HOE43478.1"
                     /translation="ERNAARIFNADHCFFVTNGTSTSNKMVWHHTVAPNDVVVVDRNC
                     HKSILHAIIMTGAIPVFLKPTRNHFGIIGPIPKHEFEPAAIKAKIAANPLVKQHLPGL
                     DANQIKPRVLTLTQSTYDGVLYNTQTVKTMLDGYVENIHFDEAWLPHAAFHPFYGTYH
                     AMGKRRARPKHAMVYATQSIHKLLAGISQASHVLVQDSQTVKLDKHLFNEAYLMHTST
                     SPQYSIIASCDVAAAMMEPPGGTALVEESIAEALDFRRAMRKIDQEYGDDWWFKVWGP
                     DKLADEGIGRADDWIIKGESRSNKSGGNWHGFGKMATGFNMLDPIKSTIVTPGMDLDG
                     KFARTGIPASVVTKFLAEHGVVVEKTGLYSLFIMFTIGITKGRWNSMLTALQQFKDDY
                     DKNQPMWRILPEFCLKHPKYEYMGLADLCQHVHQLYAKYDIARLTTEMYLSDLAPAMK
                     PSDAYAHIAQRLTERVEIDELEGRVTVGLVTPYPPGIPLLIPGEVFNKKIVDYLKFSR
                     EFNTQCPGFETDIHGLVEVVRPDGQVHYFADCVKP"
BASE COUNT          704 a         1019 c         1095 g          816 t
ORIGIN      
        1 gctatgccca atggctggct acagcgcttg acatcgacca acagttggcc tggctgaccg
       61 gcatgatgca gcgcctgggc gagttgctga ttggtcaagt cgacccactg gcattggccg
      121 agatcgaggc actgccgcac ctgcccggtg gtcgctggga gcgcgaaaag cggctgattg
      181 gtttttctga aggccagatc accgccgaac tggcgcgccg ctggaacttt ccgatgcaga
      241 ttgtgcaggc gcttgagcgc gtttatgacc ccctggttga gcaggctttt tccagaatgg
      301 gggcggtcgt taacctggct ggcttgctgg cggacacgcc tgccgcgtct gccgaaacag
      361 tcgatgactt gccgcacgat gtgatcgccg cactagggct ggatgtgcgc tggatgaagg
      421 ccactttccc ggcggctgag tcatttgtca atgtcagcct ggcttcatga aacccgctga
      481 cattgatgcg tttttttcga cgctggctgc cagcaacccg ctgccgcaga ctgaactggc
      541 atttgccagc gtattcgagt tgctcactgc cgttttgctg tccgcccagg ccactgatgt
      601 cagcgtcaac aaagtcaccc agcagctttt cctgctagct ggcactccgc aaaaaatgct
      661 ggcgctcggg ctggaagggt tggagtcttg catcaagacc atcggtctgt accatgccaa
      721 ggcgcgccac ttgatgcaaa cctgtcaaat gttgcttgat ctgcatgatg ggcaagtgcc
      781 acgcacccgc gaggcactcg aagccctgcc cggcgtggga cgaaaaaccg ccaatgtggt
      841 gctcaatgtg gcttttggcg agccaaccat ggcggttgac acccacattt tccgcctggc
      901 caaccgcacc gggctggcac ctgggacaac gccacgtgcg gtggagcaag cgttgctgaa
      961 acaggttccc gcacaatttc agcctgatgc ccatcactgg cttattctgc atggacgcta
     1021 tgtctgcaag gcgcgtacac cgcgctgctg gcagtgttca ttggcaaagt attgcgactt
     1081 caaacccaaa cctactggcc caacatcatg acgctgacac caagcaacgc tgcgcacccc
     1141 gacctgagcg cttttttgac aaccctggat tggcctgtga tgcccgaggt gggtcaggcg
     1201 ctgctgcgaa catttgccga cgaagatgcc gacattgaca cagtgtcccg catcatcagc
     1261 aaagatcccg cgctgactgc cacgctgctg cgcatggcca acagtgccat gttcggcctg
     1321 tcgggcacgg tggacaccct ggaccgggct atcagtgtgg tcggcatgtc actgattcgg
     1381 gcccgcgcgc tgtcgatttg cctggctcgt ctggtcaatc tgcccgctgg catggatcgg
     1441 atggcgttct ggcgctattg catgctgtgt gcgggatatt cacagtggct ggcggcccag
     1501 tgcgaagcag acgtgcaaga ggcgtggctg gctggcatga tgctgcggct gggcgagatc
     1561 agtctgtgca gggttcgtcc ggaggttttg ccacagttgc aggccaaacc gggtgagccc
     1621 ggcgtgcgtt ggtcgctgca gcgacacttg cttggatttg acgagggcca ggtggtcgcc
     1681 gagatggcca ggcactggga ttttccaggc acgctggtgc aaggtttgca aaacgcagcc
     1741 cagccgctgt tggcccaaaa gatgtcaaaa ctggcagcgg ttttacatct ggccgcgcgc
     1801 ctggcagaca gcgacggggt caatgaagcc agcctggatg ccttgccatt gatggtggtg
     1861 ctgctgttga aacttgatct taaagcgctg cttaccagcg cgcccgatgc cgatgtgctg
     1921 gctgatatca cgatgtttca gcactgaccg gcaaaaaaaa acaccgggaa acggcgtacg
     1981 cggggtcggc agtatgtttt tcagggtttc acgcaatccg caaagtagtg aacctgcccg
     2041 tcgggtctga ccacttccac cagcccatga atatcagtct caaatccggg gcattgggtg
     2101 ttgaactcgc gtgaaaactt caggtagtca acgattttct tgttgaacac ctcacccgga
     2161 ataagcaacg gaatgccggg cggatacggg gtaacgagcc cgaccgtcac acgcccctcc
     2221 agctcgtcta tttccacccg ctcggtcaag cgctgcgcaa tgtgtgcgta ggcatcgctg
     2281 ggcttcatgg ccggagccag gtcgctcagg tacatttcgg tggttaagcg ggcaatgtcg
     2341 tacttggcat acaactggtg cacatgctga cacaggtcag ccaggcccat gtattcgtat
     2401 ttgggatgct tcaggcaaaa ctcgggcagg atgcgccaca tcggctggtt cttgtcgtag
     2461 tcgtccttga actgttgaag cgccgtcaac atgctgttcc agcggccctt tgtaatgccg
     2521 atcgtgaaca tgatgaataa actgtagagc ccggtcttct caaccaccac gccatgttcg
     2581 gccagaaact tggtcacgac cgacgcggga atgccggtcc gggcaaattt gccgtcgagg
     2641 tccatgccgg gcgtgacgat ggtcgatttg atcggatcaa gcatgttgaa accggttgcc
     2701 attttgccaa atccgtgcca gttgccaccg gacttgttgc tgcgcgattc gcccttgatg
     2761 atccagtcgt cggcgcgacc aatgccttcg tcggccagtt tgtccggacc ccagaccttg
     2821 aaccaccagt cgtcgccgta ttcctggtca atctttcgca tggcgcgacg aaaatccagc
     2881 gcctcggcaa tgctctcctc caccaatgcg gtcccacccg gtggttccat catggctgcg
     2941 gccacatcgc aactggcgat gatgctgtat tgcgggctgg tgctggtgtg catcagatag
     3001 gcctcattga acaggtgctt gtcgagtttg acggtttgtg aatcctgcac cagcacatgg
     3061 ctggcctggc tgatgccggc caacagctta tggatcgatt gagtggcgta caccatggcg
     3121 tgttttggtc gtgcgcgtcg cttgcccatg gcgtgatagg tgccgtaaaa cggatgaaac
     3181 gccgcgtgcg gcagccaggc ctcgtcaaag tggatgttct ccacataacc gtcgagcatg
     3241 gttttgacag tttgggtgtt gtaaagcacc ccgtcgtaag tggactgggt cagggtcagc
     3301 acccggggtt tgatctggtt ggcatcaagg cccggcagat gctgtttgac cagcgggttg
     3361 gcggcaatct tggctttgat ggcggccggc tcaaattcgt gtttggggat cggaccgatg
     3421 atgccaaagt ggttgcgcgt gggtttcaga aacaccggaa tggcaccggt catgatgatg
     3481 gcgtgcaaaa tcgatttgtg acaattgcgg tccaccacca ccacatcgtt cggtgccacc
     3541 gtgtggtgcc agaccatctt gttgctggtg ctggtgccat tggtgacaaa aaagcagtgg
     3601 tcggcgttga agatgcgcgc cgcattgcgc tcgc
//
LOCUS       DAOSLL010000508         3630 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_22462_length_3630_cov_3.810070, whole genome shotgun sequence.
ACCESSION   DAOSLL010000508 DAOSLL010000000
VERSION     DAOSLL010000508.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22462_length_3630_cov_3.810070"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="PLB25_17890"
     CDS             complement(<1..897)
                     /locus_tag="PLB25_17890"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HOE43479.1"
                     /translation="MLIKRITVGEVIVPLKVPFKTALRTVNELNDLVLIVETDTGHTG
                     YGAATATAVITGDLLDSMRAALALIAPHLLGKSLLDFNTLLRTIHQSIVHHDSLKAAL
                     EIALFDLRAQQVGLPLYRLLGGGEPVIKTDITISVNDTDIMVADARRAVAEGFDCLKI
                     KLGGHAWRDDVLSVVAIFRAVGPNVALRLDANQGWSPKQAVQVIQALEKADIALELVE
                     QPVKASDVVGLKFVTEHTLTPIVADEAVFCVSDALHLLVTHSADIINIKLMKCGGISQ
                     AIEVANLTRMHHKTCMLGCMLEG"
     gene            complement(1290..>3630)
                     /locus_tag="PLB25_17895"
     CDS             complement(1290..>3630)
                     /locus_tag="PLB25_17895"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE43480.1"
                     /translation="VILASTLAALAAVGYTAHATSQRAYLASQTRLNELLDTIEGTLA
                     VACFAKDQTLAGELAQGLLSNSDVLGVSIATGQETLTDMHRGQSSGPPAMLLKRQVYS
                     PFDRHKQVGEILLTPDPLVIEARIREEVWFAAIQLSWQLAMVTFAVVVMMLLFIVRPM
                     KAISDRLHQMDPAAGERLATPQGHASTEIGQLVEDINALSDRLVSALATERKLRLQGE
                     IDEKRYHTIFDNAESGLFLIDSQGLLTSWNPAFARLFSILPQPLPNDPFVLTIGQLPW
                     QSPSQMSELVRSAVEQNQAVAQDFQYVNPQGQLNWINMVLSPVGDNLLQGVVHDVSRL
                     KESEAYARRQVITDSLTGLVNRLGLEERLQAHLQEFAATKIGGFTLLMINLDEFRQVN
                     EGMGLPAGDNILQSTTSRLSACIKSEDTLARLAADNFCIILSGVTQGEVVERIANRIL
                     QAIRQTYFVDGSPVNLHASIGITLFPNDGFDVPMLLRQTELALDSAKSAGGDTQVFFD
                     PTLTEAVEYRRHLESDLRSAIQKNELVLFFQPIIDLNAQRLSGAEALIRWRHPTRGLV
                     SPDSFIPLAEKVGLIAEIGLFVVDRACQQLLSWKNQGLDYTLSLNVSGRQIPDRLSPD
                     MLNSVVQRYGISPDRLALEITEGVMLHDVEKSLQWLGAVHRMGFRVYLDDFGTGYSSL
                     SYLKLFPVDTLKVDKSFVQDMQDDSNEYKLVAAIIAMGRGLGLEIVAEGVELKSHLTA
                     LKRMGCHYAQGYYFSQPVPAEEFAAAAARVAELLAQPET"
BASE COUNT          808 a         1053 c         1004 g          765 t
ORIGIN      
        1 accttcgagc atacatccca gcatgcaggt cttgtggtgc atgcgcgtca ggttggccac
       61 ctcgatcgcc tggctgatgc cgccgcattt catgagcttg atgttgatga tgtccgcact
      121 gtgggtcacc agcaggtgca atgcatcgct gacacaaaac accgcctcat cggccactat
      181 gggtgtcagc gtgtgttcag tgacaaactt gaggccaacc acatcagacg ccttcaccgg
      241 ttgctcgacc aattcaagcg caatatcagc cttttcaagc gcctgtatca cctgcaccgc
      301 ctgtttcggg ctccaaccct gattggcatc aaggcgcagc gccacgttgg gcccaaccgc
      361 gcggaaaatc gccaccacgc tgagcacatc atctcgccag gcgtgcccgc ccaatttgat
      421 tttgaggcag tcaaagcctt cagccacagc ccgcctggca tcggccacca tgatgtcggt
      481 gtcattgaca ctgatggtga tgtcggtctt gatcaccggc tctccaccgc cgagcaaccg
      541 gtacaaaggc agaccgacct gttgggcgcg caggtcaaac agcgcaatct ccagtgccgc
      601 tttcaggctg tcgtggtgaa caatcgactg gtgaatggtg cgcagcaggg tgttgaaatc
      661 aagcagactc ttgccaagca agtgaggcgc aatcaaagcc agcgcagcgc gcatcgagtc
      721 aagcaaatcg cccgtgatga ccgccgtggc agtggccgca ccatagccgg tgtgaccggt
      781 gtcggtttcg acaatcagca ccaggtcatt gagttcattg accgtgcgca gtgcggtttt
      841 gaagggaact ttaaggggaa caatgacttc gccgacggta atgcgcttga tcaacatgtg
      901 aacgtttcta aaagttgact ggtttggtca gtcgggaagc aacgttcaaa ttgtagtaaa
      961 gcccgcgtcg gcgaaaagca cggatcacca gcagcagcaa ccctttgcgt ggtgtcagca
     1021 aagcagccat tgcagattga gttcagccgg cgccatgtca gcagagcgtc gcactggttt
     1081 ttgcatacag ggcgtaagcc gcgccagttc caagttcaca ctcatatgcc aaataggcct
     1141 ctagcgcccg tcccataagc gctacaagct ataacttaaa tagcaaatat aaaatgttgg
     1201 caggcaaagc cgactgcgct gctgattggc caacaaagcg cgccagtcaa tcacacgccg
     1261 tcttgccagt caaacccacc gcgccgactt caggtttccg gctgcgccag caattccgcc
     1321 acccgtgccg ccgccgcagc gaattcttct gctggtaccg gttgcgaaaa gtaataacct
     1381 tgcgcatagt ggcatcccat gcgtttgagt gccgtcagat gacttttcag ttcgacccct
     1441 tctgcgacga tctccaggcc caggccgcga cccatggcga tgatggccgc aaccagtttg
     1501 tattcgttgc tgtcgtcctg catgtcctgt acaaaggatt tgtccacttt cagcgtatcc
     1561 accggaaaca acttgagata ggacagcgac gagtagccgg tgccaaagtc atcaagataa
     1621 acgcgaaagc ccatcctgtg aacggcacca agccattgca gcgacttttc gacatcgtgc
     1681 agcatcacgc cttcggtgat ttccagcgcc agccggtccg gcgaaatgcc atatcgctga
     1741 accaccgaat tgagcatgtc aggcgacagg cgatccggaa tctgtcggcc cgaaacattc
     1801 agcgacaggg tgtagtccaa gccctggttt ttccagctca gcagttgctg acaagcgcga
     1861 tccaccacga acaggccgat ctcggcaatc aggccgactt tttctgccag tggaatgaag
     1921 ctgtcgggcg acaccagccc acgggtcgga tgtcgccaac ggatcagcgc ctcggctcca
     1981 gacaagcgct gcgcgttcaa gtcgatgatg ggctggaaaa agagcaccaa ttcattcttc
     2041 tggatggcgc tgcgcaagtc gctttccagg tgtctgcggt attccaccgc ttcggtcagt
     2101 gtcgggtcaa aaaacacttg cgtgtcgcca ccggcggatt tggcactgtc cagggcgagt
     2161 tctgtctggc gcaacagcat tggcacgtcg aatccgtcgt ttggaaacag cgtgatgccg
     2221 atgctggcat gcaggttgac tggcgaacca tcaacgaaat aggtttgtcg aatcgcctgc
     2281 aagatgcggt tggcaatccg ttcaaccacc tcaccctggg tgacaccact gaggatgatg
     2341 cagaagttgt cggccgccag gcgcgccagg gtatcttcac ttttgatgca ggctgacagt
     2401 cggctggtgg tgctctgcaa gatgttgtca ccggccggca ggcccatgcc ttcattgact
     2461 tggcggaact cgtccagatt gatcatcagc agggtaaaac cgcctatttt tgtcgcggcg
     2521 aattcctgca gatgagcctg caggcgctct tccagcccga gacggttgac cagaccggtc
     2581 agagagtcgg tgatgacctg gcgccttgca taggcttcag actctttgag gcgactgaca
     2641 tcgtgcacca cgccttgcaa aaggttgtca ccaaccggac tgagcaccat gttgatccag
     2701 ttaagctggc cttgcgggtt gacatactga aagtcttgtg ccacagcctg gttttgctcc
     2761 acggcgctgc gcaccagttc actcatctgg ctcgggcttt gccagggcag ttgtccgata
     2821 gtgagcacaa acggatcgtt cggcaagggt tgcggaagaa tggaaaacaa gcgcgcaaag
     2881 gccggattcc aggaagtcag caaaccctgg ctgtcgatca agaacaaccc ggattcggca
     2941 ttgtcgaaaa tcgtgtggta gcgcttttcg tcgatttcac cttgcagacg caatttgcgt
     3001 tccgtcgcca aggcgctgac cagacgatca gacagcgcat tgatgtcctc gaccaactgg
     3061 cctatttcag tgctggcatg tccttgtggt gtcgccagac gctcacccgc agcaggatcc
     3121 atctggtgca agcggtcgga aatggccttc atcgggcgca cgatgaacag cagcatcatc
     3181 accaccacgg caaacgtgac catggccaac tgccagctca attgaattgc ggcaaaccag
     3241 acttcttctc tgatccgggc ttcaatcacc agtgggtcag gggttaacaa gatttcgcca
     3301 acctgcttat gccggtcgaa cggcgaatac acctggcgct tgagcagcat ggcaggtggg
     3361 ccactggatt gaccgcgatg catgtcggtg agcgtctcct gcccagtcgc aatgctgact
     3421 cccagcacat cggaattgct cagcaaaccc tgcgccagtt ccccggccag ggtctgatcc
     3481 ttggcgaaac acgccacggc gagcgtgccc tcgatggtgt caagcagttc attcaggcgg
     3541 gtctgcgatg ccaggtaggc acgttggctg gtggcatggg cggtgtatcc aaccgcggcc
     3601 agagcggcca gtgtcgatgc aagaataacc
//
LOCUS       DAOSLL010000509         3626 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_22489_length_3626_cov_4.177541, whole genome shotgun sequence.
ACCESSION   DAOSLL010000509 DAOSLL010000000
VERSION     DAOSLL010000509.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3626
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22489_length_3626_cov_4.177541"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /locus_tag="PLB25_17900"
     CDS             <1..1285
                     /locus_tag="PLB25_17900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOE43481.1"
                     /translation="PFTLKSAALVGALCCADLASAQSSVTLYGLADAGITSVSGLKGG
                     TVTQLASGIMEGSRWGLKGTEDMGGGYKAIFTLEARVELDTGASSNRPISGSQLSDRY
                     SQATLLGLTSSSATLQGAYQAAVSGVDALLAAQYGVNVTGNVFDRQAFVGLITPVGGI
                     LAGRQYTPAYETFGTYDIMATQSGLSAGQIVQFPAVFEIRASNALAYRIAQGGLSGSL
                     MYAPGESTQGGSQNRLVGFNLGYKTGVFSVGLGYNSKNNELGKKSLTSSVVGASMNLG
                     NSSFSTLAAKITDDHPAGLSVISASLLPSLAGLGSLAGPTADKIQSAFIDAFKQDGTL
                     FHIGYRYVTGPHTASVAYNRYNDSRPADADVASFGVAYSYALSKRTDLNAVLVRYNNK
                     NLAQVAPGGNGYLGGVTASAGTDSTGLALGVRHRF"
     gene            complement(1307..1636)
                     /locus_tag="PLB25_17905"
     CDS             complement(1307..1636)
                     /locus_tag="PLB25_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43482.1"
                     /translation="MHHADYKPMFCHAVTVRNLAQPLALDCWRQSLDDSLLEKVPWSC
                     EADYANQLDANSRGTRWGGSKQFQFAIKYKAIGALCISATARFDQQMVGSMGDRRTTK
                     KPLGRGF"
     gene            1800..2420
                     /gene="coq7"
                     /locus_tag="PLB25_17910"
     CDS             1800..2420
                     /gene="coq7"
                     /locus_tag="PLB25_17910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone
                     monooxygenase"
                     /protein_id="HOE43483.1"
                     /translation="MIDRLLSTADAALRTIFASHSAAQACPTLAGDVTELSEAEKQQA
                     GALMRVNHVGEVCAQALYTAQAFATQNEALRAHFMRASDEETNHLAWTEARLKELGDR
                     TSLLNPLWYAGAFGIGLLAGRLGDRVSLGFVVETENQVEAHLDSHLSLLPAGDHASRA
                     IVAQMKEDEARHALEAQELGAMDLPAPAKALMKLAAKLMTTVAHRV"
     gene            2859..3086
                     /locus_tag="PLB25_17915"
     CDS             2859..3086
                     /locus_tag="PLB25_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43484.1"
                     /translation="MLDLPAHSPAMGQNTGANRAVAVGLQPQRINTAGSKIHGHPTIY
                     CHDAEGFFNSLHTILEPGFLSWLLVFICKFL"
     gene            complement(3277..>3626)
                     /locus_tag="PLB25_17920"
     CDS             complement(3277..>3626)
                     /locus_tag="PLB25_17920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome B"
                     /protein_id="HOE43485.1"
                     /translation="FLGLQVASGMFSDDEIAAAGPLVRFASSTWVGNATFYHKEIGKL
                     ILLALVALHLAAISFYFFKKGENLVRPMVTGDKTLDFPATSSSDQSADRTRAAVIILL
                     CAALVSGFIVWLG"
BASE COUNT          739 a         1026 c         1041 g          820 t
ORIGIN      
        1 gccctttacc ctcaaatccg cagcccttgt cggcgccctg tgctgcgccg atctggccag
       61 cgcccaatcc agtgtcacac tgtatggcct ggccgatgct ggcattacct cggtcagcgg
      121 cttgaaaggc ggcaccgtga cgcagcttgc cagcggcatc atggaaggct cgcgctgggg
      181 tctcaagggc accgaagaca tgggcggcgg ttacaaggcg atttttaccc tggaagcccg
      241 ggttgaactc gataccggcg catcaagcaa cagacccatt tcgggcagcc agttgtcaga
      301 ccgttactca caggcgacct tgctgggcct tacctcgtca tctgcaacgc tacaaggtgc
      361 ttaccaggcg gccgttagcg gtgttgatgc cttgttggcg gcgcaatatg gtgtcaatgt
      421 gacaggcaat gtgtttgacc ggcaagcttt tgtcggtctg atcacgcctg tgggtggcat
      481 tctggccggt cgtcagtaca caccggctta tgaaaccttt ggcacatacg acatcatggc
      541 cacccaatct ggcctgtcag caggccagat tgtgcagttc ccggcggtgt ttgaaatccg
      601 tgcgtccaat gcgctggcct accggattgc tcaaggtggc ctctcaggtt ccttgatgta
      661 tgcacctggc gaatcaacgc aaggtggtag tcagaaccga ctggtgggct tcaatttggg
      721 ttacaaaacc ggcgttttct cggtcggcct tggttacaac tccaaaaaca acgaattggg
      781 gaagaagtcc ctgacttcat ccgtggtggg tgcatcgatg aatcttggca acagttcgtt
      841 cagtacgctg gcagccaaga tcactgacga tcaccccgcc ggtctttcgg tgatttctgc
      901 ttccctgttg ccttctttgg ctggcctggg ttctttggcg ggaccaacgg ctgacaagat
      961 tcagtcggcg tttattgatg ccttcaagca agacgggacg ctgttccaca tcggctatcg
     1021 ctatgtcact ggcccgcata cggcttcagt ggcttacaac cgttataacg acagccgtcc
     1081 ggctgatgcc gatgttgcgt cattcggggt ggcctacagc tatgcgctgt ccaaacgcac
     1141 cgacctgaat gccgtgctgg ttcgttataa caacaagaac ctcgcccagg tggcacccgg
     1201 cggtaatggc tacctgggtg gcgtgaccgc gtccgctggc accgactcca ctggcctggc
     1261 attgggtgta cgccaccggt tctgattttt ggcagccact gcttcctcaa aagccccttc
     1321 cgaggggctt ttttgttgtc cgccgatcgc ccattgaacc caccatttgt tgatcaaatc
     1381 gtgctgtagc gcttatgcat aaagcgccaa tagctttgta tttgatagca aactggaatt
     1441 gcttcgagcc gccccatcgg gtacctctgg agttggcatc aagctgattg gcatagtcgg
     1501 cttcacaaga ccatggcacc ttttccagca ggctgtcgtc cagactttgc cgccagcagt
     1561 cgagtgccaa cggctgggcc aggttgcgca ccgtgaccgc atgacagaac atcggcttgt
     1621 agtcggcatg gtgcaaagcg gacttctgac atcggcggac ggtcactgat ctgactgacc
     1681 ggaggtggct atgtcacgat ggtgggcatg acaaacctag ggccaacttt ccgttggacg
     1741 gctctacaat ttatccgggt ctgcccgtcc atttcacttc aagagccaag aaacacgcca
     1801 tgatcgaccg acttctgagc actgccgatg cggcgctgcg caccatcttt gcaagtcaca
     1861 gcgctgcgca ggcctgcccg accctggcag gtgatgtcac tgagctgtct gaagctgaga
     1921 agcaacaggc cggtgcgctg atgcgcgtca atcatgttgg cgaggtgtgt gcccaggcgc
     1981 tctacacggc gcaagccttt gcaacacaaa atgaagcact tcgcgcccat ttcatgaggg
     2041 ccagtgacga agaaaccaat caccttgcgt ggaccgaggc acgtttgaag gaactgggcg
     2101 accgcacctc gctgctcaac ccgctgtggt atgcaggtgc ctttggcatc gggttgctgg
     2161 ctgggcggct gggtgaccgg gtcagcctgg ggtttgtggt ggagaccgaa aaccaggtcg
     2221 aagcgcacct ggacagccat ctgagccttt tgcccgcagg cgaccatgca tcacgggcca
     2281 tcgtggcgca aatgaaagaa gatgaagcgc gtcatgcact cgaagcccag gaacttggcg
     2341 cgatggattt gcctgcgccg gcaaaagcgc tgatgaagct ggctgccaag ctgatgacca
     2401 ccgtggcgca ccgggtttag ctggctacgc aagcggtttg caacaacgca tagcgcttgt
     2461 cagcgcagcc gaactgatca agtcattttt gtctgacacc ttagccttcg gtgacgacaa
     2521 aacgaactca aagtcttttc tggtaccccc aggtcaaggt gcgtgttgac catcgcctgt
     2581 acaacgggcg ctttgttgag caggatgtgg gcatctggtg gcgtcctttg ctgcgcttgc
     2641 cgtggcgacc gaatccgcgc tggtcatgcc tgaatgccac cgtgccaagt ctctggcaac
     2701 tgttgcattc actgacaagt tggacatcgt tctgcgtgga ttttgcagtt ttcaggttcc
     2761 agcgtcagag ttgacatcct tgccttggtt tgggccagca cgaggtcgga tttgtggatc
     2821 gagcagcgag aacctacccc ggcaaccccc ggcgagcggt gctggatttg cctgcccata
     2881 gcccggcgat gggtcaaaac accggtgcaa atagagcggt tgcggtcggt ttgcagccgc
     2941 agcgcatcaa tacggcaggc tcaaagattc atggtcaccc aaccatctat tgccacgatg
     3001 ctgagggctt tttcaattct ctgcacacca tccttgaacc cggattcctc agttggctgc
     3061 ttgtattcat ctgcaagttc ttatgagcat ttacttttca gcttcgatgg ggttcaccca
     3121 ttgggtgaca acgttaatcg cacgattgaa tcactggcgg cgcgcctggt ggcatccaac
     3181 cgaggaatcc aggttgaaca ggttgaacag gttgaaacgg gggataggga gcagtttgcg
     3241 cacgctgctc cgctggcgtg caggtcaagc ctgccgttac ccgagccata cgataaagcc
     3301 actgaccagg gccgcgcaaa gcaggatgat gacggcggct ctggtgcggt cagcgctttg
     3361 gtcgctcgaa ctcgtcgccg ggaaatccag tgtcttgtca ccggtcacca tgggcctgac
     3421 caggttctcg ccctttttaa aaaaatagaa actgatggca gccaggtgca gcgcgaccag
     3481 cgccagcaaa atcagtttgc cgatttcctt gtggtagaaa gttgcgttgc cgacccaggt
     3541 ggacgaagcg aaccgtacca gcggtcctgc ggcagcaatc tcgtcatcac tgaacatgcc
     3601 ggaggcaacc tgcaatccca ggaacc
//
LOCUS       DAOSLL010000510         3624 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_22507_length_3624_cov_2.632390, whole genome shotgun sequence.
ACCESSION   DAOSLL010000510 DAOSLL010000000
VERSION     DAOSLL010000510.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22507_length_3624_cov_2.632390"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PLB25_17925"
     CDS             complement(<1..246)
                     /locus_tag="PLB25_17925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxD N-terminal domain-containing protein"
                     /protein_id="HOE43486.1"
                     /translation="MKLDLATIVASLGGELHGDGAIVVHGLAPLEIATDCELSFLSNP
                     RYQSQLVASKAACVIVAPAMQSIAVARGACIVTDQPYV"
     gene            complement(271..780)
                     /locus_tag="PLB25_17930"
     CDS             complement(271..780)
                     /locus_tag="PLB25_17930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019700071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HOE43487.1"
                     /translation="MITSKRLWLATVLLAGCAISATAQDFKVGYINTQAITTQSNPAK
                     AAQSKLEQEFSKRQKELTDLQASLKSFGEKFDRDAPTLSESQRSARQKEGAEMSRDLQ
                     RRQREFQEDLNGRRNEELQLVLDRATKAVKQVADAEKYDMVLQEVVYVNAKHDITEKV
                     LKILNSGSK"
     gene            complement(780..3077)
                     /gene="bamA"
                     /locus_tag="PLB25_17935"
     CDS             complement(780..3077)
                     /gene="bamA"
                     /locus_tag="PLB25_17935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HOE43488.1"
                     /translation="MQFNRNFLRRVSVAAASLFVIQGAWALEPFTVKDIRVEGLQRAE
                     AGTIFASLPVRVGDTYSDEKAAASIKALFGLGLFKDVRIEVNGDVLVVIVEERPTIAE
                     MEFVGAKEFDKDVLKKALAQIGLTDGRPFDKALVDRAEQELKRQYINRSMYASEVITT
                     VTPVERNRVNLSFAVVEGEPAKISDIRIVGSKAFSESTLRGLFDLDTGGWLSWYTKSD
                     RYARAKLNADLEALRSYYLARGYLEFKVESTQVAISPNKQDIGITINVSEGDQFVVSK
                     VKLEGNFLGREDEFRSLVAIQAGQPYNADQVAVTSKAFTDHFGNFGYAFSRIEPRTVI
                     DRTTNRVEVTLLADPSRRVYVRRINVAGNDRTRDEVIRREFRQLEASWYDGEKIKLSR
                     NRVDRLGYFKEVTMDTSEVPGSFDQVDLTVTVSEKPTGSISLGAGISSADGFGLSFGF
                     KQDNAFGSGNSLGIELNTSKQNRTLVFNTTNPYFTEDGVSRTIDVYQRNTKPYVDINS
                     YAIETTGGSVRFGVPFTEYDTVFFGLGIDQTTVVPGTLLPVVYQDFANRFGFTSSAVP
                     LTVGWGRDTRDSALVPTIGKVIKTSAEWSIAGELKYVRATANYQHYIPVTNKITGAFN
                     AELSVGSGTGDTPYPIFKNFYSGGLGSVRGFEQGSLTTGPQRTLGLTSTGGTKKINFN
                     AELLSPLPGGGKDRTLRLYAFMDVGGLYGPDEAIQFSDLRSSYGVGISWISPVGPLRL
                     AFAKPIRQFENDRIQSLQFQIGSSF"
     gene            complement(3125..>3624)
                     /locus_tag="PLB25_17940"
     CDS             complement(3125..>3624)
                     /locus_tag="PLB25_17940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011801156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HOE43489.1"
                     /translation="QISVLPRLVQEGEVSFGRIGAVVGAPPAMTLIQYGLLDGVGEAI
                     RRTWETSFLTLRVMGQIVIGQASLKNLSGPVTIADYAGKSAAMGFSQFLSFLALMSVS
                     LGVLNLLPLPVLDGGHLMYYLWEALSGKPVAPFWTEQLQKGGLVILLMMMSVAIVNDV
                     TRLLP"
BASE COUNT          892 a         1027 c          846 g          859 t
ORIGIN      
        1 cacatagggc tggtccgtga cgatgcacgc cccacgtgcc accgcgatgg attgcatcgc
       61 cggtgccacg atcacacatg ccgcctttga agcaacgagc tgagattggt acctgggatt
      121 gcttagaaaa ctaagttcgc aatcggttgc gatctcgagc ggagccaaac catgcaccac
      181 gatggctccg tcaccatgca actcgcctcc caaagaggca acaatcgtcg ccaggtcaag
      241 tttcattctc caggcccgtt cgcccaaggc tcacttggag ccggagttga gaatcttcaa
      301 aaccttttca gtaatgtcat gcttggcatt gacgtagacc acttcctgca acaccatgtc
      361 gtacttttcg gcgtccgcaa cctgcttcac agcctttgtc gcccggtcca gcaccagctg
      421 caactcttcg ttgcggcgac cattcaggtc ttcctgaaac tcccgctgac gacgttgcaa
      481 gtcacgcgac atttcagcgc cttctttttg ccgtgcactg cgttgactct cgctcaatgt
      541 gggcgcgtcc cggtcaaatt tttcaccaaa acttttcagc gacgcttgca gatcggtcaa
      601 ctctttctgg cgcttcgaga attcttgctc aagcttggac tgtgcggctt tggcaggatt
      661 ggattgcgta gtgatggcct gagtgttgat gtaacccact ttgaaatcct gggcggttgc
      721 gctgatggcg cagccagcaa gcagaacagt agccaaccag agacgttttg aggtaatcat
      781 taaaaggagc ttccaatctg gaattgcaga gactgaattc tatcgttttc aaattgcctg
      841 atcggcttgg caaacgccag tcgcagtggg ccaaccggtg atatccagct gatacctacg
      901 ccgtacgagc tgcgcagatc ggaaaactga atcgcctcgt caggcccata cagtccacca
      961 acatccatga atgcatatag gcgcagggtt ctatccttac caccaccggg aaggggcgat
     1021 agcaattcgg cattgaagtt gatcttcttc gtaccacctg ttgaagtcaa acccagtgtt
     1081 cgctgggggc cagttgtcaa acttccctgt tcaaatcctc gaaccgagcc caagccgccg
     1141 gaataaaagt tcttgaaaat gggatatggt gtgtcgccgg tacctgaccc gaccgacaat
     1201 tccgcattga atgcgcctgt gattttgttg gtcacgggaa tgtagtgctg gtagttggcc
     1261 gtcgcacgaa cgtacttcag ttctcctgca atactccatt cagcggaggt cttgatcacc
     1321 ttgccgatcg tcgggaccaa tgcactgtca cgcgtgtcgc gcccccaacc gacggtcaga
     1381 ggtacggcgc tactggtaaa accaaatcgg ttggcaaagt cctggtacac caccggcaac
     1441 agcgtgcctg gcaccactgt agtctggtcg attccgaggc caaaaaatac agtgtcgtat
     1501 tcagtaaatg gcacaccgaa gcgcacacta ccgccggttg tttcaatggc atagctattg
     1561 atatcaacat agggctttgt gttgcgctga tatacgtcaa tggttctcga aacaccgtct
     1621 tctgtgaagt agggattcgt tgtattgaaa accaaggttc gattctgctt actggtattg
     1681 agttcgatac cgagagaatt tcccgaacca aacgcattgt cctgcttgaa tccaaacgaa
     1741 aggccgaatc catcggcact cgagatacct gcacccaaac tgatactgcc cgtgggtttt
     1801 tcagatacgg taacggtcaa gtccacctga tcaaaggacc cagggacttc ggatgtgtcc
     1861 atcgtgactt ctttgaaata cccaagcctg tccacacgat ttcttgagag cttgattttt
     1921 tccccgtcgt accatgacgc ctcaagctgg cgaaactcgc ggcgtatcac ttcatcacgt
     1981 gttcgatcat ttccagcgac attgatccgg cgcacataca ctcggcgcga cggatcagcc
     2041 agcagcgtta cctcgacccg attagtcgtg cgatcgatca cggtgcgagg ttcaatacgt
     2101 gaaaaagcat agccaaaatt accgaaatgg tcggtaaatg ctttcgacgt tacagccact
     2161 tgatcagcgt tgtatggctg ccctgcctga attgcaacca acgaacgaaa ctcatcttct
     2221 ctgcccaaaa agttgccttc aagtttgact ttggaaacta caaactgatc cccttcgctc
     2281 acattgatcg ttatgccaat gtcctgctta ttgggggaaa tcgcaacctg tgtggattcc
     2341 accttgaatt ccaaatatcc acgcgccagg tagtaagagc gcaaggcctc cagatccgca
     2401 ttcagcttgg cccgcgcata tcggtcagac tttgtgtacc aacttagcca accaccggta
     2461 tccagatcaa acaaccctcg caacgtggac tcagaaaacg ccttgctgcc aactatgcgg
     2521 atgtcgctaa ttttggctgg ctctccctcc accacggcaa aactcaggtt tacacgattt
     2581 cgctcaacag gggtcacggt ggtgatcact tcagacgcat acatgctgcg attgatgtac
     2641 tgacgcttca actcctgctc ggcccgatca accaacgctt tgtcaaacgg ccttccatcc
     2701 gtcaagccga tttgggctag cgcctttttg agaacatcct tgtcgaattc cttagctccg
     2761 acgaactcca tttctgcaat ggtgggacgc tcttcaacaa tgacaaccag cacgtcgccg
     2821 ttgacctcaa tgcgcacatc cttgaacaac ccgaggccaa acagcgcctt tatcgatgct
     2881 gccgcttttt catcgctata ggtatcgcca acacgtacgg gcaaagaggc gaaaatcgtt
     2941 ccggcttccg ccctttgcag tccctcaacc cgaatatcct tcactgtaaa tggctctaaa
     3001 gcccacgcac cttgaatcac gaaaagcgac gccgccgcaa cggacacacg gcgcagaaaa
     3061 ttacgattga attgcatttt ttttaaattt aagtagtccg gaaggttgag gacaggccga
     3121 atggtcatgg cagcaaacgc gtcacgtcgt taacaattgc aacggacatc atcatcaaca
     3181 ggatgaccag gccccctttt tgcaattgtt cagtccaaaa cggcgctacc ggcttcccgg
     3241 aaagtgcttc ccaaagataa tacatcaggt gccctccatc aagcactggc aacggtaaca
     3301 ggttgagaac gcccaaactc acgctcatga gtgcaagaaa tgacaaaaac tggctgaaac
     3361 ccatagcggc agattttcca gcgtagtcgg cgattgttac tggaccactg agatttttga
     3421 gcgaagcttg accgatcacg atttgcccca tcacacgcag ggtcagaaat gatgtctccc
     3481 aagttctgcg aatagcctcg cccaccccat ccagtagccc atattgaatc agcgtcatcg
     3541 caggtggcgc accgacaacg gcaccaatgc gaccaaaaga gacttcccct tcttgcacga
     3601 gtctgggtaa tacagaaatt tgtc
//
LOCUS       DAOSLL010000511         3585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_22795_length_3585_cov_3.917847, whole genome shotgun sequence.
ACCESSION   DAOSLL010000511 DAOSLL010000000
VERSION     DAOSLL010000511.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22795_length_3585_cov_3.917847"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="PLB25_17945"
     CDS             complement(<1..891)
                     /locus_tag="PLB25_17945"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoglycerate dehydrogenase"
                     /protein_id="HOE43490.1"
                     /translation="MYKVRTYNQISVKGLERFPRQSYEVGSDIGSPDAFLLRSQKLQG
                     VEVPKSLLTVARAGAGVNNVPVDLYGKQGIVVFNTPGANANAVKELVMAGMLLATRGI
                     LPGMAYVNSLISLTDAAEMAQLLEKEKSRFAGGEIKGKTLGIVGLGAIGSMVAEMALA
                     MGMKVVGFDPALSIDAAWRLSSEVSRMENLQSLLARADYISMHVPAMDATRHLINEDA
                     LTMVRTGAVLLNFARETIVDSSAVLRSLDKGKLGKYVCDFPEPALLNHPKVIAMPHIG
                     ASTEESEENCAVMAANQLMDY"
     gene            complement(901..2016)
                     /locus_tag="PLB25_17950"
     CDS             complement(901..2016)
                     /locus_tag="PLB25_17950"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF002841.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HOE43491.1"
                     /translation="MQPTVKPKNPNFSSGPCAKRPGYDVSKLQLDTLGRSHRSALGKK
                     ALGLVCTETARLLGLPEGYRVAVVPGSDTGAVEMAMWSLLGQRGVDVLEWESFSAGWV
                     TDIVKQLKLPNVNVRRADYGDIADLSAVDFANDVVFVWNGTTSGVKVPNGDWIPADRA
                     GLTLCDATSAVFAMALPWEKLDVVTYSWQKVLGGEGAHGMLILSPRAVARLESYSPPW
                     PMPKVFRMKSGGKISEGLFKGETINTPSMLCVADYLDALKWVESLGGVAGTIARSEAN
                     LRVIEAFVAANDWISFLAKSPATRSNTSVCLTVAATEEQVKKMVKLLDAEGVAHDMGA
                     YKDAPAGLRIWCGATVESSDLQALMPWLTWAYQQVTA"
     gene            2151..2303
                     /locus_tag="PLB25_17955"
     CDS             2151..2303
                     /locus_tag="PLB25_17955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43492.1"
                     /translation="MPEARQRLMSKHKQKAYRAAESRQMATKKQRKLAFMTNFIKGKQ
                     LVASAG"
     gene            2456..>2708
                     /locus_tag="PLB25_17960"
     CDS             2456..>2708
                     /locus_tag="PLB25_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43493.1"
                     /translation="MRKRIIAPTQQNAAPLDEHWLNLEALAEVEITSEDSAHPVEFAL
                     LPGHAAGWRAAGVGTQTMRLLFAQPQALRRVWLHFAEHQL"
     assembly_gap    2709..2718
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2719..2902
                     /locus_tag="PLB25_17965"
     CDS             <2719..2902
                     /locus_tag="PLB25_17965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43494.1"
                     /translation="GGTTYREIVRQQWNFSPDGASSEIEDHRCELPAVTQLELIINPD
                     ISGNDAIASLEALRLA"
     gene            complement(3035..3526)
                     /locus_tag="PLB25_17970"
                     /pseudo
     CDS             complement(3035..3526)
                     /locus_tag="PLB25_17970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236184.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          752 a         1057 c         1007 g          759 t
ORIGIN      
        1 gtagtccatg agctggttgg cggccatcac cgcacaattt tcttccgact cttcggtgct
       61 ggcaccgatg tgcggcatgg cgataacctt ggggtggttc aacagtgccg gttcggggaa
      121 gtcgcagaca tatttgccca gcttgccttt gtcgaggctg cgcagcacgg cgcttgaatc
      181 gacaatggtc tcgcgcgcga agttgagcag cacggcaccg gttctgacca tcgtcagcgc
      241 atcctcattg atcaggtgtc gggttgcgtc catagctggc acatgcatcg agatgtagtc
      301 agcgcgggcc agcaaagact gcaggttttc catgcggctg acttcactcg acagtcgcca
      361 ggctgcatcg atggacagcg ccgggtcaaa gccgaccacc ttcataccca tcgccagcgc
      421 catctcggcg accattgagc caatcgcccc gagccccaca atgcccagcg tcttgccctt
      481 gatctctccg ccagcaaagc gggatttttc tttttcaagc agttgcgcca tttcggcggc
      541 atcggtcagg ctgatcaacg agttgacata tgccataccc ggcagaatgc cgcgtgtagc
      601 cagcagcata cccgccatca ccagttcctt gacggcgttg gcgttggccc ctggggtgtt
      661 gaacaccaca atgccctgtt tgccgtacag atcgaccggc acattgttga cacctgcacc
      721 ggcgcgcgcc accgtcagca aggacttggg gacttccaca ccctgcaact tctggctgcg
      781 cagcaaaaac gcatcgggac tgccgatgtc gctgccgacc tcatacgact ggcgcggaaa
      841 gcgctccagg cccttgaccg aaatctggtt gtaagttctg actttgtaca tgttgtggcc
      901 tcaggcggtt acttgttggt aagcccatgt gagccagggc atcagcgcct gcagatcaga
      961 cgactcgacg gtggctccac accagatgcg cagaccggct ggcgcgtcct tgtaggcacc
     1021 catgtcatgc gcgacacctt cggcgtcgag cagcttgacc atcttcttga cctgctcctc
     1081 ggtggctgcc accgtcagac acacgctggt attggagcgg gtggccgggc ttttggccaa
     1141 aaaagaaatc cagtcattgg ctgcaacaaa ggcttcgatc accctcaagt tggcctcgct
     1201 gcgtgcgatg gtgccggcaa cgccgcccag gctctcgacc cacttcagtg cgtccaggta
     1261 gtccgccacg cacaacatcg acggtgtgtt gatggtttcg cccttgaaca ggccttcact
     1321 gatctttccg cctgatttca tgcgaaacac cttcggcatc ggccatggcg ggctatacga
     1381 ctcaagacgg gccaccgcac gcgggctcag aatcagcatg ccgtgggcac cttcgccgcc
     1441 cagcaccttt tgccaagaat aggtcaccac atcgagcttc tcccagggca acgccatggc
     1501 aaacaccgcc gaggttgcat cacacaaagt caggccggcc cggtcagcgg gaatccagtc
     1561 gccattgggc accttgaccc cggaggtggt gccattccag acaaacacca cgtcgttggc
     1621 aaaatcaacg gcggacaaat cggcaatatc gccatagtcg gcgcgacgca cgttgacatt
     1681 gggtagcttg agctgcttga cgatgtcggt tacccaaccc gcgctgaacg actcccactc
     1741 cagcacatca acaccacgct gtccaagcag cgaccacatg gccatctcga ccgcaccggt
     1801 gtcagagcca ggcaccaccg ccacccggta accctcaggt agacccaata ggcgggccgt
     1861 ctcggtgcag accagcccga gggctttttt gcccaaagcc gagcggtgtg aacggcccag
     1921 agtgtcaagt tggagcttgc ttacatcgta gcctgggcgc ttggcacagg gcccggaaga
     1981 aaaattggga ttttttggtt tgacggtagg ttgcatgcta tttcagtcag tagaaaaaaa
     2041 ttgattgtat tgtccccggc gaggccattt tttgcggaaa agctctgccg taggctgtct
     2101 cggcctgttc aaacatcggc aatccatcat ggatcggtca caatcagcac atgcccgaag
     2161 cgcgacaacg cctgatgtct aagcacaaac aaaaagctta ccgcgcagca gaaagccgcc
     2221 agatggccac caaaaaacag cgcaaacttg cattcatgac caatttcatc aaagggaaac
     2281 agttggtggc cagtgctggt tgatggcatg ccgacggatg gtgatgcgtc cgagtactga
     2341 tccgatttgg ctgtatcagt acgaggcgtg gtagagcaca ggctgagttt ttgtccgtag
     2401 caacggtgtt tggggccact gagttttcaa cattcattat ggaaaaggac atccgatgcg
     2461 caaacgtatc atcgctccga cgcagcaaaa cgcggccccg cttgacgaac attggctgaa
     2521 tctggaggcc ttggccgagg tggagatcac ctcggaggac agcgctcatc cggttgagtt
     2581 tgccttgctg cccggtcatg cagcgggctg gcgcgcggct ggagtcggca cgcagaccat
     2641 gcgactgctg tttgcccagc ctcaagcact gcggcgtgtc tggctgcact ttgccgagca
     2701 tcagcttgnn nnnnnnnnag ggggaaccac ttatcgcgaa atcgttcgcc agcagtggaa
     2761 tttcagccct gatggtgcca gctccgagat agaagatcac cggtgcgaac ttccggcagt
     2821 cacccagctt gagttgatca tcaatcccga catcagcggg aacgacgcaa ttgcctctct
     2881 tgaggcattg cggctggcct gacttacccg gacaaccagg ctacgggctg cggcttgtag
     2941 agtagttagc gactgcgcgg gccagaatca acccgaagca ttgtcaagca aatcagcgca
     3001 ataggtagaa aaccgcccat ctttgtgcgt cgtaggcctg cttctcgtca aactcagcct
     3061 tctcgctggc gtagcgctgt gttgcacgtg ctgcaatttt ggccctggcc gctgccatgg
     3121 cagcgagccg gtcctggcgc agcttgagtt ccttgggcag gctcatgcga tcgggaatat
     3181 tggctgtgtc ggcttgttca gccagggcca gcagctcctg gacttccttt attagctgct
     3241 cttcgatctt gacaatgtgc ccatgtgaga ttgcactgtg gcgtgaggca ctggcatgaa
     3301 ttttgctgcc atccagacac accgtgccca tcttgagcag cttcatctct ttgcccatct
     3361 ccagcacttg caaaaagagt cccgtgagtt catcaacgaa gcgccttcga aaggtggcca
     3421 gggtgtcctg gtcggggtgg ctgcctgcag cgatgtagcg aaaggcgacc gagtcatagg
     3481 tggccagctc cagtttgcgg ctggagaaca cgcctgttga atacccttag aatgtggtcg
     3541 ggaataactc cccgttttgc accaaggagt tgggatgcac ggcta
//
LOCUS       DAOSLL010000512         3577 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_22863_length_3577_cov_3.937252, whole genome shotgun sequence.
ACCESSION   DAOSLL010000512 DAOSLL010000000
VERSION     DAOSLL010000512.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22863_length_3577_cov_3.937252"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="PLB25_17975"
     CDS             <1..622
                     /locus_tag="PLB25_17975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003067855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE43495.1"
                     /translation="LTGLYTPDTGSFELAGKTYRPTAVHEVAKAGIARTFQNIRLFSE
                     MTALENVMVGRHVRTHSGLLGAVFRTAGFRQEEAAIAARSQELLDYVGIGRFADFKAR
                     TLSYGDQRRLEIARALATDPQLIALDEPAAGMNSTEKILLRELIDKIRNDNRTILLIE
                     HDVKLVMGLCDRVTVLDYGKQIADGTPADVQRNEKVIEAYLGTSGH"
     gene            670..1386
                     /locus_tag="PLB25_17980"
     CDS             670..1386
                     /locus_tag="PLB25_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE43496.1"
                     /translation="MSNVFLKVTGLKVAYGGIQAVKGVDFEVREGELVSLIGSNGAGK
                     TTTMKAITGSLPINDGDIEYLGQSIRGQGPWDLVRQGLAMVPEGRGIFTRMTILENLQ
                     MGAYIRNDKAEIALDIDKVFGIFPRLKERRDQLAGTLSGGEQQMLAMGRALISRPKVL
                     LLDEPSMGLSPLMVDKIFEVVREVYEQGVTVLLVEQNASRALAIADRGYVMDSGLITM
                     TGDGKTMLKDPKVRAAYLGE"
     gene            1670..2053
                     /locus_tag="PLB25_17985"
     CDS             1670..2053
                     /locus_tag="PLB25_17985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HOE43497.1"
                     /translation="MTKRLISSGSTFEEQIGYSRAVVVGDWVFVSGTTGFDYKTMSIS
                     ESLVQQTEQCLKNIESALQQADSSLKDVVRVTYVLPDGAEFEQCWPVLRKYFGEVRPA
                     AMMISAGLADPRMKIEIEVTALKRS"
     gene            2116..2262
                     /locus_tag="PLB25_17990"
     CDS             2116..2262
                     /locus_tag="PLB25_17990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43498.1"
                     /translation="MKLRYFFDAGSGVCLWAGDDEVQVRYDYPVGLDILPISETLRIA
                     GATH"
     gene            2507..3256
                     /locus_tag="PLB25_17995"
     CDS             2507..3256
                     /locus_tag="PLB25_17995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016354310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE43499.1"
                     /translation="MISSQFASQVSLRREDVESFDRYPFCLPAVRSLETLELHPKVTF
                     LVGENGSGKSTLLEAIAVSLGFNPEGGTKNFRFGTRRSHSELHAFIRVAKGFRRPKDG
                     FFLRAESFFNVATEIENLDAEPSFGPPVIDAYGGRSLHEQSHGESFLALMMNRFGADG
                     IYILDEPEAALSPQRQLSVLSRIHDLVNTNSQFIIATHSPLLMAYPESWIYSCSASGL
                     SRVCYQDTEHYQVSRDFLANPERMLKVLLER"
     gene            3382..>3577
                     /locus_tag="PLB25_18000"
     CDS             3382..>3577
                     /locus_tag="PLB25_18000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43500.1"
                     /translation="MPSRTTLNANQVRELGRATFSGTLSALASKVRAWQRHRPVNPKQ
                     AYLPPPSFKKSPLSPPSDSMP"
BASE COUNT          765 a         1008 c         1012 g          792 t
ORIGIN      
        1 gctgaccggc ttgtacacgc ctgacaccgg tagctttgag ctggccggca aaacctatcg
       61 gcccactgcg gtgcatgagg tggccaaggc tggcattgcg cgcacgttcc agaacattcg
      121 cttgttttcc gagatgacag cgctggaaaa tgtcatggtc ggacggcatg tgcgcactca
      181 ctcgggtttg ttgggtgcgg tgtttcgcac tgccggtttc aggcaggagg aagcggccat
      241 cgccgctcgc tcgcaagaac tgctcgacta cgtgggcatt ggccgttttg ctgatttcaa
      301 ggcacgcacc ctgagttacg gtgaccagcg tcggctggag attgcgcgtg cgctggccac
      361 cgacccccaa ttgattgcgc tggacgaacc tgctgcaggc atgaacagca ccgaaaaaat
      421 tctgctgcgc gagctgattg acaagattcg caacgacaac cggaccattt tgctgatcga
      481 gcacgatgtc aagctggtta tgggactgtg tgaccgggtc acggtactcg attacggcaa
      541 gcagattgcc gatggcacgc cggccgatgt gcagcgcaac gaaaaggtga tcgaagccta
      601 tctgggcacc agcggccact gaaacagcca ggtagcgcca aacatctgtc ccacaaggta
      661 agcatcatta tgagcaatgt atttttgaaa gtcaccggcc tcaaggttgc ctatggcggc
      721 attcaggcgg tcaagggcgt tgactttgaa gtccgcgagg gcgaactggt gtcgttgatc
      781 ggttccaacg gtgctggcaa gaccaccacc atgaaagcca tcaccgggtc gttgccaatc
      841 aatgacggtg acatcgagta cctgggccag agcattcgcg gccagggacc gtgggacctg
      901 gtccgacaag ggctggcgat ggtacccgag ggtcgtggca tttttacccg catgacaatc
      961 ctggaaaacc tgcagatggg tgcctacatt cgcaacgaca aggccgagat cgcgctcgat
     1021 atcgacaagg tgtttggcat ttttccccgt ctgaaagagc gccgcgacca gttggcgggc
     1081 acgctcagcg gtggcgagca gcaaatgctg gcaatgggcc gcgccctgat cagccgaccc
     1141 aaggtgctgc tgctcgacga gccgtccatg ggcctgtcac cgctgatggt ggacaagatt
     1201 tttgaagtgg tgcgcgaggt ttacgagcaa ggtgtcacgg tgctgctggt tgagcaaaat
     1261 gccagccgtg cgctggccat cgccgaccgg ggttatgtga tggattccgg cctgatcacc
     1321 atgaccgggg acggcaagac catgctgaaa gaccccaagg tgcgcgccgc ctatctgggc
     1381 gagtgattca tcgttcgcct tcgggtgcct tggtgtctgg catttgaaca atgaagcaag
     1441 aattgaacga ggtgtggcgg cgggtgagca gcgcttgcgg tcattctgtc agccagtcca
     1501 gccagttaat ccaaaggcct ggccatgcca ccggctgtca ccttgagcac atgggtgtca
     1561 atactggacg ctcacaccga ccgcctgata gcggctgagg ggctatatct accgagccga
     1621 tcaagtcgca ctacaggccc gtctctcgcc gcgcttactt ggaaatcgaa tgaccaaaag
     1681 actcatcagc tcaggctcca cctttgaaga gcagattggc tattcgcgcg cagtcgtcgt
     1741 cggcgactgg gtgtttgttt ctggcacaac cggcttcgac tacaagacca tgtccatctc
     1801 ggagagtctt gtccagcaaa cggagcaatg cctgaaaaac attgagtccg cgctgcagca
     1861 agccgactca agtctcaaag atgtcgtgcg ggtaacgtat gtcctgcctg atggtgctga
     1921 gtttgagcaa tgttggccgg tgctacgcaa gtatttcgga gaagtgcgtc cggctgccat
     1981 gatgatctcc gctggtttgg cagacccacg catgaagatc gaaatagaag tcacggccct
     2041 gaagcgttca taggcgtgcg cgttcttcat cggtcagata ctcttggctg gcatgttcaa
     2101 ggcagagggt acagcttgaa gcttcgatac ttctttgatg caggctctgg cgtatgcttg
     2161 tgggccggtg acgacgaagt acaagttcga tacgactatc cagtcggttt ggacatcctg
     2221 ccgatttccg agaccttgcg cattgcggga gcgactcatt gaaactcgga acacttccat
     2281 tgattgggac tgtccgggaa gcccgagtac ctggactgcg tcaaagcgcg cagagttcct
     2341 tcgcaggagc gatccatttc atcgcgctct ccgtaacgaa ccaggttcag atttcgaggt
     2401 gatgaatgag gtgcgcggat aacatgtcat tcgacactga cccacaacag cggaaagctg
     2461 cttcgctgca cgttgtcgtg gcccggcctt tttcacgttg agtgtcatga tctcatctca
     2521 gtttgcgagc caggtcagtc tgcgaagaga ggatgtcgag tcctttgacc gctatccctt
     2581 ctgcttgccc gccgtgcgat ctttggagac attggagctt catccaaaag tgacattcct
     2641 ggttggcgaa aatggttccg gaaagtccac gcttcttgag gccatagctg tctcacttgg
     2701 attcaatccc gaaggcggaa cgaagaactt tcgtttcggc actcgtcgat ctcactcgga
     2761 gttgcatgcg ttcattcggg ttgcgaaggg ctttcgccgc cccaaggacg gcttcttcct
     2821 tcgtgccgag agtttcttca atgttgcgac ggaaatcgaa aaccttgacg ctgaaccctc
     2881 cttcggccca ccggtcatcg acgcctacgg ggggcgctcg cttcatgaac aatcccacgg
     2941 cgagtcgttt ctggcattga tgatgaatcg ctttggcgca gatggcatct acatcctgga
     3001 cgaaccggag gccgcgctct cgccgcagcg acagttgtca gtcctttccc gaattcacga
     3061 tctcgtcaac accaattccc agttcatcat cgccacacat tcgccgttgc tcatggccta
     3121 tccagagtcc tggatctact cctgttctgc gtccggcttg tcgcgcgttt gctaccagga
     3181 cactgagcac tatcaggtaa gccgtgactt tttggccaat ccagaaagaa tgctaaaagt
     3241 cttgctcgaa cgctaagatg ccaatccatc gctcaacggg actgccgaat agctgcgctg
     3301 ctcggtaaac ttcgcccttt aggctgcggt cagtccctca actcaaacct tgggcctcag
     3361 aaaccacctg cttttgcgct cttgccatcc cgaacaaccc tgaacgcaaa ccaagtccgc
     3421 gaactgggca gagccacctt ttctggcacg ctgtcggcat tggcgagcaa ggtgagagca
     3481 tggcagcgac accggccagt caatcccaaa caggcctatt tgccgccacc cagcttcaaa
     3541 aagtcaccac tttcaccccc cagcgacagc atgccgc
//
LOCUS       DAOSLL010000513         3571 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_22918_length_3571_cov_6.714164, whole genome shotgun sequence.
ACCESSION   DAOSLL010000513 DAOSLL010000000
VERSION     DAOSLL010000513.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22918_length_3571_cov_6.714164"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="PLB25_18005"
     CDS             complement(<1..183)
                     /locus_tag="PLB25_18005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase N-terminal
                     domain-containing protein"
                     /protein_id="HOE43501.1"
                     /translation="MAIYTPPLRDMQFVMHELLNVTADLKAMPSHADMDADTINAVLE
                     EGGKFAADVLLPLNISG"
     gene            complement(212..901)
                     /locus_tag="PLB25_18010"
     CDS             complement(212..901)
                     /locus_tag="PLB25_18010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HOE43502.1"
                     /translation="MSQSISISKTTRAVSAGVSRGERAMQKGQQTKQLVIDAALGLAE
                     QIGLEGLSIGALAEVTRMSKSGVFAHFGSREELQISVVREYYKRFSDEVFFPAMDEPR
                     GLPRVRAMFANWMKRVAVEIQSGCIFISGAVEFDDRPGPVRDALVSSVRTWLAAMHRA
                     VVQAKEEGHLVSDSDEAQMVFEIHGLILALHYEAQFLKNPGAIGRANTGFENILLRYG
                     KFAARPLSNPN"
     gene            1198..2922
                     /locus_tag="PLB25_18015"
     CDS             1198..2922
                     /locus_tag="PLB25_18015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HOE43503.1"
                     /translation="MFIKVTLSGNRRYAQLVESFRNEEGKPRQRTVCTLGRLEAGGEV
                     DTLIASLQRARGLATSSTSASPLDGLRFTDSRHFGDIWALSELWCSLGFDDLASSWRR
                     SKTEVDVLSCLRLMVFNRLCDPGSKLGVLRWLETVALPSGFGVVSEHQHLLRAMDVLD
                     EHSDKLKLRLATLMRPLIDQDLSVVFYDLTTVAVTGQTDLEDDIRAYGLAKSGLVERQ
                     FMLSLVQTAEGLPIAHEVHPGNTAEAKTLLPMIRGLLARYPLKCVVLIADRGLLSTAN
                     IEELDKLQAQLKKDGRDVAVEYILAVPAARYGDFADDLQKLHKSQDATQEWCAETKWN
                     DSRLVVAHDPVAANRRSVARDKTIAELVKLGQQCSVKLDEQDESQRTGKKNKSKGRPM
                     SDSGTKARFYHAVKDAHMAHLIKVDLKAELFSYTIDEDKKRYLELLDGKLLLVTNTSS
                     PAAEVVQRYKSLADIERGFRVLKSDIEIGPVYHRLPQRIRAHALICFMALVLYRVMRM
                     RLKAARRSESPSTLLEQLKRIHQQTVETSEGATLTGLTEITPAQKSLFAALDLAPPTP
                     SDLTKPVL"
BASE COUNT          797 a         1014 c          979 g          781 t
ORIGIN      
        1 accactgatg ttgagcggca gcaacacgtc ggcggcaaac ttgccacctt cttccagcac
       61 cgcattgatg gtgtcggcgt ccatgtcggc atgcgaaggc atggccttca ggtcagcggt
      121 tacattgagc agctcgtgca tcacaaactg catgtcgcgt agcggggggg tatagatggc
      181 catggtgtct ctcctagaaa gttgaagtga ttcagtttgg gttggacagg ggccgcgcgg
      241 cgaacttgcc atagcgcagc aaaatattct caaaaccggt gttggcccgg ccgattgcgc
      301 ccgggttttt caaaaactgc gcctcgtagt gcagcgccag aatcaggcca tggatctcaa
      361 acaccatttg tgcctcgtcg gaatcgctga ccagatgccc ctcttccttt gcctgcacaa
      421 cggcgcggtg catggcagcc agccaggtcc gaactgaact caccagcgcg tcgcgtactg
      481 gcccgggccg gtcgtcaaat tcaaccgcac cactgataaa gatacagcca gactgaattt
      541 caaccgctac ccgcttcatc cagttggcaa acatcgcacg cacccgcggc aggcctcttg
      601 gttcatccat ggccggaaaa aacacttcgt cagaaaagcg tttgtagtac tcacgcacaa
      661 cggatatctg caactcttcc cgcgacccga aatgtgcaaa tacacccgac ttgctcatcc
      721 gggtgacctc tgccagcgca ccaatgctca gcccttccag cccaatctgc tcagccagcc
      781 ccagggccgc gtcaataacg agctgtttag tctgctggcc cttctgcatg gcccgctcac
      841 cgcgcgatac accggcgctt acagcacgag tggttttgga aatggaaatt gattggctca
      901 tgttacataa aagaacgatc gttctatttt gcagcatttc ccaaaaaagg gaagcccaac
      961 cgggtgttct agtgtgggcc tactagggaa ttcgcgggtt taccctagac gcactagcgg
     1021 tcaaaggccc cagaagttcg acaccttttt gccaagattc cggtttttgt gccccggcct
     1081 gctaatgaat tcaagtactt accccaattt tcaggagcaa agcaaaagat gtctgtagtg
     1141 gcacgttgcg tcttttaagt agttttcgta tttcaagtct gagctacact cagtgccatg
     1201 ttcatcaagg tcaccctatc gggcaatcga cgctacgccc agctggtgga atccttccgc
     1261 aacgaggaag gcaagccccg ccagcgcacc gtctgcaccc tgggtcgcct ggaagccggt
     1321 ggcgaagtcg acaccctgat tgcctccttg cagcgtgccc gcggcctggc aacttcgtcg
     1381 acttctgcct cgcctcttga tggtctgcgc tttaccgaca gccgccactt tggagacatc
     1441 tgggccttgt ctgaactctg gtgctcgctg ggatttgacg atctggctag ctcctggcgg
     1501 cgctccaaaa ccgaggtgga tgtcctgagt tgcctgcgcc tgatggtctt taaccgcctg
     1561 tgtgatcctg gcagcaaact cggtgtgctg cgctggctgg aaacagtggc tttaccttcg
     1621 ggctttggtg tcgtctctga acaccagcac ctgctgcgcg ccatggatgt gctcgatgag
     1681 cacagcgaca aactcaagct ccgtctggcc acactcatgc gccccctgat tgaccaagac
     1741 ctctcggtgg tgttttatga cctcaccacg gtggcggtca ctggccagac ggaccttgaa
     1801 gatgacattc gtgcttacgg tctggccaag tcgggtctgg tggagcgcca gttcatgttg
     1861 tccctggtgc aaaccgctga gggactgccc attgcgcatg aagtgcatcc cggcaacacg
     1921 gcagaggcta aaaccctgtt gccgatgatt cgaggtttat tggcccgcta cccgctcaaa
     1981 tgtgtggtgc tgattgccga ccgggggctg ctcagcaccg ccaacatcga agaattagac
     2041 aagctgcaag cccagctcaa aaaagacggc cgtgatgtcg ccgtggagta catcctggcg
     2101 gtgccggcag cacgatatgg tgactttgca gatgacctgc aaaagttgca caagagccag
     2161 gacgccaccc aggaatggtg cgccgagacc aaatggaacg atagccgtct ggtggtggca
     2221 cacgatccgg tggcggccaa caggcgctca gtagcgcgag acaaaacgat tgctgaattg
     2281 gtcaagctgg gccagcagtg cagtgtcaaa cttgatgagc aggacgagag ccagcgcacg
     2341 ggcaaaaaga acaaaagcaa agggcgaccg atgtcggact cgggcaccaa ggcgcgtttc
     2401 taccatgcag tcaaagacgc ccacatggcg catctgatca aggtggactt gaaggctgag
     2461 ctgttcagct acacgattga cgaagacaaa aaacgttacc tggaactgct tgatggcaag
     2521 ctgctgctgg tgaccaatac gagttcaccg gctgctgagg tggtgcagcg ttacaagagc
     2581 ttggcggaca ttgagcgggg ctttcgggta ctgaaatcag acatcgagat tggcccggtg
     2641 tatcaccggc tgccacagcg cattcgcgcg catgcgctga tttgtttcat ggcgttggtc
     2701 ctgtaccggg tgatgcgcat gcggctcaag gcggccaggc gttccgagtc gccctccacg
     2761 ttgctggagc aactcaagcg gattcatcag cagactgttg agacaagtga gggggccaca
     2821 ctgaccggct tgactgagat cacgcccgcc cagaagtcac tatttgcggc cttggatttg
     2881 gcaccaccaa ccccttcaga cctcaccaag cccgttttgt agtctgcggt tcaacttcca
     2941 tttctaatgg aatcaatctc ttacacgcag gaagtgtcga acttgtgcgg ccaacgtctg
     3001 tttgtcaatg gcctctgccc tcattgacga attgctcgtg acatagcagc cagttgatcc
     3061 ctggcttcca gagaccgctt tggagcaggc agatcgtaaa actgtaagac cgtaaccgct
     3121 gcgatgcagg tctacagttt tctccattgt tgacattcaa ttttgacggt cagaccccga
     3181 gaaagttggc cggctccaaa aggctggttc cggccattcg cccaaatagc aaaaatctca
     3241 acaaatagtg ggaaagctgc ccttcaattt cgccggaagc agtcgctcgc tgccaggggc
     3301 aaaagcttcg aagcagtcgg tcaaatgggg ttgcctgttt tcttggctgt ccggctcttg
     3361 agcagcgtca gaagtcattg gaattttggc ggtgaatggc tccataaaac acacgggagt
     3421 cagtcgactt ccggaaattc catccccatg gccgaccgtc aagtcacgga ttcacgccca
     3481 gcaatatggc ggtgatttcg tccaaccgca caagcgctgt tcggcagagt gtgaacagcg
     3541 gagttcatca ctggcgctat tcaaagggca c
//
LOCUS       DAOSLL010000514         3570 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_22925_length_3570_cov_3.752774, whole genome shotgun sequence.
ACCESSION   DAOSLL010000514 DAOSLL010000000
VERSION     DAOSLL010000514.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3570
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22925_length_3570_cov_3.752774"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            346..570
                     /locus_tag="PLB25_18020"
     CDS             346..570
                     /locus_tag="PLB25_18020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HOE43504.1"
                     /translation="MNITLSIDEQVAQRAREAAQKMGKSLNQVVRDYLEQLAGGARRG
                     QQWSQFEERCLRSEARLDGWHFNRDEANVR"
     gene            573..986
                     /locus_tag="PLB25_18025"
     CDS             573..986
                     /locus_tag="PLB25_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HOE43505.1"
                     /translation="MATRSFIDTNVLVYAEASDEPKKQRAALALLKSLYDENLGVLST
                     QVLQEYCNVALKKLKLPAQYVRAQLDLYEQFEIVQVTPHIIRAGLDLHQTRSMAFFDA
                     IVLASAHAAGCNVIWTEDMNTGEIVNGVCISNPFK"
     gene            complement(1054..1355)
                     /locus_tag="PLB25_18030"
                     /pseudo
     CDS             complement(1054..1355)
                     /locus_tag="PLB25_18030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003150546.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative toxin-antitoxin system toxin component,
                     PIN family"
     gene            complement(1355..1594)
                     /locus_tag="PLB25_18035"
     CDS             complement(1355..1594)
                     /locus_tag="PLB25_18035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013105051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HOE43506.1"
                     /translation="MDSVRWNIAVSSETDQSVRMFIAAQGRGRKGDLSRFIEEDVRSY
                     LLEKAVEQAKSAATGMGESELTDLIDEAVQWAREH"
     gene            1799..1879
                     /locus_tag="PLB25_18040"
     CDS             1799..1879
                     /locus_tag="PLB25_18040"
                     /inference="COORDINATES: protein motif:HMM:NF012464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOE43507.1"
                     /translation="MKIGYARVSTRDQNLNLQLDALNQAG"
     gene            complement(2030..3097)
                     /locus_tag="PLB25_18045"
     CDS             complement(2030..3097)
                     /locus_tag="PLB25_18045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011811859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HOE43508.1"
                     /translation="MLTTHNILITPEILALISKLDEFKGAWRALGTLAPDRLLALRRV
                     ATIESIGSSTRIEGSRLSDQAVQALLAKIELKSFATPGEQEVAGYADVMELLFSSWEH
                     IPFNENHIMQIHRDLLAYSSNDERHRGQYKTHENRVAAFDENGHEIATIFQTASPFDT
                     PRLMRELLEWVRHTRDGQALHPLLIVAVFVVVFLEIHPFDDGNGRLSRILTTLLLMQA
                     GYVYVPYSSLESVIEQNKQAYYMALRQTQGTIRTDAPNWQPWITFFLSSLVSQVKRLE
                     VKIEREHLLLATLPKLSIDLLTIAREHGRLTVASAQAQTGANRHTIKLHIQILIAAGQ
                     LTLRGVGRATWYELKHADTPK"
     gene            complement(3348..>3570)
                     /locus_tag="PLB25_18050"
     CDS             complement(3348..>3570)
                     /locus_tag="PLB25_18050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43509.1"
                     /translation="SVLVVSDPPHMLRLRYAWFSNFWGSDLSYTLIATNPPWWSAWRW
                     WANPQSSSFVENEVVKLGYYLLHHRFGVF"
BASE COUNT          884 a          972 c          951 g          763 t
ORIGIN      
        1 accatggcgt gtgaccagtg tcgagctcaa cacggcgcaa aaagcgcctc gacttcaagc
       61 tcacccaaaa cgatgccagt cagagccacc ctagcctatc aaatactaca tattcaatag
      121 catcctgcgc ttactggata agggccagat ggtgattttg tgtatagcta ccagaaggtt
      181 ttgctgaccc agtgcagacg tacatccgga gatctgtcga tgcccgccct ggtcatcgat
      241 ggtgcagcga tgaataaagg gtagcgtccc ctgttttcgc atttttcgac gcaaatcgca
      301 cataatatgt gcgcacacaa gccatttaat cacaggaact ccagcatgaa catcacgctt
      361 tcgattgacg aacaagttgc acaacgtgcc agagaagcag cccaaaaaat gggcaagagc
      421 ctgaaccagg ttgtgcgcga ttacctggag caactggccg gcggcgcgcg ccgtggccag
      481 caatggtcgc agtttgaaga acgatgcctg cgctctgagg cacggctcga cggctggcat
      541 ttcaaccgcg acgaagccaa tgtgcgttga acatggccac acgcagcttt atcgatacca
      601 atgtgctggt ttacgctgaa gccagtgacg agccgaaaaa gcagcgtgcc gcccttgcgc
      661 tgctaaagag cctctatgac gaaaatcttg gggtactgtc aacccaggtt ttgcaggaat
      721 actgcaacgt agcgctcaag aaactcaagc tacctgcgca gtatgtgcgc gctcagcttg
      781 atctgtatga gcaatttgaa attgtgcagg tcacgccaca tatcatccgg gctggcctgg
      841 accttcatca gacccgcagc atggcattct ttgatgccat cgtgctcgcc agcgcccatg
      901 ctgctggctg caacgtgatc tggacagagg acatgaatac tggcgaaatt gtcaatggtg
      961 tctgcatctc gaacccgttt aaataaagga aataaagcgg acgctaccct agcgttcccg
     1021 ctgtgtggca gggaggaaac tgaccggcat tgatcaaagc acttcagcgc agaaagtggc
     1081 cggggtgacg atgcgcgcgc gaccggcagt gcctaattgc agcagcccaa cacgatggcg
     1141 cgctgcatgt tgttgaccat cgtgccgaca cggtgggcag gcaatatggt tttgagcttg
     1201 ggataacggc tcacgcgccg caattcgtcg agctgtgttg tcgaggttac caaatcaaac
     1261 ttggccgcgc gccacgagcg atagatcacg tcaggcggtc cgtgcggcga aatcagcgcg
     1321 tcaagcaaca cattggtgtc caggataacg cgcatcaatg ttcacgcgcc cattgcactg
     1381 cctcgtcgat gaggtcagtc agttcggact cgcccatgcc ggtggcagcc gatttggcct
     1441 gctccacggc cttctccaaa aggtaggagc gtacatcctc ctcaatgaat cgtgatagat
     1501 cgcccttgcg gccacgaccc tgcgcggcga tgaacatgcg cacagactga tccgtctcag
     1561 acgacacggc gatattccag cggactgaat ccataataat ttcggtgttt gagtgcatag
     1621 gtatttattc acttaataga atcaccatgc aagtaagttc gataaaacaa tcgttttatg
     1681 aaactttagc aatccataaa tcgtgcaagc ttgctgtggt catatcaggc tcacaatcgt
     1741 ggtttgataa atcaaagttt tgatttgatc aaaatcaaac gagtaaagaa tacttttgat
     1801 gaaaatcggc tacgcccgcg taagcacgcg cgaccaaaac ctcaacttgc agttggatgc
     1861 cctcaaccaa gcagggtgag aacgtattgg ccaagatgtg gccagcggtt cgaaagcggc
     1921 gcggcctgca ttggacgaac tgcttggcca aatccgagct ggggacgtat tggtgtgcaa
     1981 atctgcccct ggagaaaatg gtaacgtccc ctttttccaa gcaaacgggt cattttgggg
     2041 tgtctgcgtg tttcagctca taccaggtag cacgccctac cccgcgaagc gtcaactgcc
     2101 cggcagcaat cagtatttga atgtgtagct tgatggtgtg gcggttggcc ccggtttgtg
     2161 cctgcgcact ggccaccgtc aagcgaccat gctcacgcgc tatcgttagc agatcgatag
     2221 aaagcttggg cagcgtggcc agcagcagat gctcgcgctc gatcttgact tccagccgct
     2281 ttacctgact cacaagcgaa ctcaaaaaga acgttatcca cggctgccag ttgggtgcgt
     2341 cggtgcggat cgtgccttgc gtttgccgca gtgccatgta gtaagcctgt ttgttttgct
     2401 cgatcacgct ctccagcgag ctgtaaggca catacacata acccgcttgc atcaacagca
     2461 acgtggtcag aatgcgcgat agtcgcccat tgccatcgtc aaacgggtga atttccaaaa
     2521 aaaccaccac aaaaacggcc acgatcaaca acggatgcag ggcttggccg tcacgcgtat
     2581 ggcgcaccca ttcaagcaac tcgcgcatca gccggggggt gtcaaatggc gaggcggtct
     2641 gaaaaatggt cgcaatttca tgaccgtttt catcaaacgc agccacccgg ttttcatgcg
     2701 ttttgtactg cccacggtgg cgctcgtcgt tactactgta ggccaacaaa tcgcggtgaa
     2761 tttgcatgat gtggttttca ttgaacggga tgtgctccca ggagctaaac agcagttcca
     2821 tcacatcggc gtaacccgcc acctcttgct cgccgggcgt ggcgaacgac ttcagttcaa
     2881 tcttcgcaag aagcgcctgg accgcttgat cagacaggcg gctgccttca atgcgggtgg
     2941 aagaaccaat gctctcaata gtggccacac ggcgcagtgc cagcaaacgg tctggtgcca
     3001 gtgtgcccaa cgcacgccag gcacccttga actcatcaag tttggagatg agcgccagaa
     3061 tctctggcgt gatcaaaatg ttatgggtcg tcagcatagc cgcaaatata gccttgaata
     3121 gccataaaag gtggtatgga tagccgtgac atggcggtgt gcaactttgc ggccccgcac
     3181 aacctccggg aacctctgcg ccatcagcgg gggctagacc accgcaagaa gagagcggct
     3241 tgaattaccg atagtcttat agtcaaatca gacgtgagcc cccgaacaac aagcgtgtac
     3301 agctattaac ttggtagcaa acgggctgct actgtgaacg cgctttgtca aaacacccca
     3361 aaccggtgat gcagcaagta ataccccagc ttcaccacct cgttttcgac aaagctgctt
     3421 gactgtgggt tggcccacca gcgccaggct gaccaccaag gcgggttggt ggctatcagg
     3481 gtgtaagaca ggtcagagcc ccagaaattc gaaaaccagg cgtaacgcag ccgcagcatg
     3541 tggggtgggt cgctcaccac caacaccgac
//
LOCUS       DAOSLL010000515         3559 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_22994_length_3559_cov_4.164098, whole genome shotgun sequence.
ACCESSION   DAOSLL010000515 DAOSLL010000000
VERSION     DAOSLL010000515.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3559)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22994_length_3559_cov_4.164098"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="PLB25_18055"
     CDS             <1..647
                     /locus_tag="PLB25_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43510.1"
                     /translation="DKQQSAPLAVPSVALPDRQPLPSNLLAAIDEPRDGASYFFNPLA
                     VEWQSFRAAQVLRYLQFFNSLVARSCLADIARYTHQIVPQFNPSWDSGKYAAQATLQP
                     TKDWRTGISLYGETTYGRSIPDWFAQSPRASYGVTEFHPLVPLTAEHLGQVINRHRSG
                     GAAFLSFFVETRWQGQKVNATPNLFSFDPDNAQFGSDQLYAGIKKLLSNQRYQQ"
     gene            885..2600
                     /gene="brxL"
                     /locus_tag="PLB25_18060"
     CDS             885..2600
                     /gene="brxL"
                     /locus_tag="PLB25_18060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease Lon-related BREX system protein BrxL"
                     /protein_id="HOE43511.1"
                     /translation="MSSYEALLSNLGVKNAEISDTYVRQFEKLLVGGIWCIVTLKYHF
                     EEDQKGSPFSVTDLKPIQMPNMDMAALFEARKAFTDAQWIDALIRSTGMEPCHFKERV
                     KWHLLARMVPLVENNYNFCELGPRGTGKSHIYKEISPNSILVSGGQTTVANLFYNMGA
                     RKVGLVGLWDVVAFDEVAGINFKDHDGVQIMKDYMASGSFSRGRESINASAAMVFVGN
                     INQSVESLVKTSHLLAPFPEAMIDSAFFDRFHAYVPGWEIRKMRPEFFTNQYGLIVDY
                     LAEYLREMRKQSFADAADKWFKLGNNLNQRDTIAVRRTTSGLLKLVCPNGEYTKDSVR
                     KCLEYALEARRRVKEQLKKIGGMEFYDVHFSYIDLEDGVERFVTVPEQSSGALVPEGR
                     LNPGTLHTISMGSTEMPGIYRLEIQSIPGTGKANVSGLAPREAVRVAFDDFKANSSRV
                     SASIKPGERDFHTHLVELQHSGAPQSFTLASFIAFCSAALGKPVQSQLAVMGDMSLGG
                     TIVQVRNLAECMQVAFDAGAKRILLPMSSVTAIATVPGELFAKFQTSFYSDPVDAVFK
                     ALGVK"
     gene            2759..>3559
                     /locus_tag="PLB25_18065"
     CDS             2759..>3559
                     /locus_tag="PLB25_18065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020703401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HOE43512.1"
                     /translation="MKLAHSTSTLRQIPPGVWVLGLVSMLMDISSEMVHSLLPMFMVT
                     VLGTSAFTVGIIEGLAESTALIVKVFSGALNDYLGKRKGLAVIGYALGALTKPLFAIA
                     SGTGIVVTARLLDRVGKGIRGAPRDALVADITPSHIRGAAFGLRQSLDTVGAFTGPLI
                     AVGLMLLWSNDFRAIFWVAVVPGLLAVALLLFGVHEPERHVGEPTSNPIRRENLSRLG
                     RSYWWVVTIGAVFTLARFSEAFLILRAQQSGVPMALVPLVMVAMNLVYA"
BASE COUNT          712 a          979 c         1028 g          840 t
ORIGIN      
        1 tggacaagca gcagagcgcc ccgctggccg tgccttcggt tgccctgcca gatcgccaac
       61 ccctgccgtc gaacctgctg gcagccattg acgagccgcg cgatggcgcg tcctattttt
      121 tcaacccgct ggcggtagag tggcaatcct ttcgcgccgc ccaggtgctg cgctatctgc
      181 agttcttcaa ctcactcgta gcccgctcgt gtctggccga tatcgcccgc tatacccacc
      241 agatcgtgcc gcagttcaat ccaagctggg acagtggtaa atatgccgca caggccacgc
      301 tgcagccaac aaaagactgg cgcaccggta tcagcctgta tggcgaaacc acctatgggc
      361 ggtcgatacc cgattggttt gcgcaaagcc cgcgcgccag ttatggggta acagaatttc
      421 acccgcttgt gcccttgacg gcagagcacc tgggtcaggt gatcaaccgg cacagaagtg
      481 gtggtgcagc gtttctgtca ttttttgtgg aaacccggtg gcaggggcaa aaggtcaatg
      541 ccacgcccaa tcttttttcg tttgaccccg acaacgcgca atttggctcg gaccaactct
      601 atgcgggcat caagaaactg ttgtcaaacc agcgctatca gcaatgaaag caatttcagc
      661 ccctttttag cttgatcttc agtttggagt gctcaatgcc ctacgccacc cacttcgcgc
      721 atagctcctt gaagcgcttg taattggcta cttttcggcg cacatctgcc acagcatcat
      781 ttgagacgta gtccgttcga ctcttcatat cgagcgtgta gctcaactgg aaataggggc
      841 tggtttggtt ctgcgggtct tcgaattgtt gcgtagacta cgcactgtcc agctatgaag
      901 ccctgctgtc aaacctggga gtcaaaaacg ccgagatttc agacacttat gtgcgccagt
      961 tcgagaagtt gctggtgggc ggcatctggt gcatcgtcac cctgaaatac cactttgagg
     1021 aagaccagaa gggttcacct ttttctgtca ccgacctcaa accgatccag atgcccaaca
     1081 tggacatggc ggcgctgttt gaggcgcgca aggcgttcac tgatgcgcag tggattgatg
     1141 cgctgattcg ctcgactggc atggagccct gccatttcaa ggagcgggtc aagtggcacc
     1201 tgctggcacg catggtgccg ctggtggaaa acaattacaa cttctgcgag ctggggccac
     1261 gcggcactgg caaaagccac atctacaagg agattagccc gaacagcatt ctggtgtcag
     1321 gcgggcaaac cacggtggcc aacctgtttt acaacatggg cgcacgcaag gtcggtctgg
     1381 taggtctttg ggacgtggtg gcattcgatg aggtggcggg catcaacttc aaggatcatg
     1441 acggcgtgca gatcatgaag gattacatgg cctcaggctc gttcagccgg ggccgcgagt
     1501 ccatcaacgc cagcgccgcc atggtatttg tcggcaacat caaccagagt gtggagtcgt
     1561 tggtcaaaac ctcgcacctg ctggcaccgt tccccgaggc gatgattgac tcggctttct
     1621 ttgaccgttt tcatgcctat gtgccgggct gggaaatccg caaaatgcgg cctgagtttt
     1681 tcaccaacca atatgggttg atcgtggatt acctggcgga gtacctgcgc gaaatgcgca
     1741 agcagagttt tgccgatgca gcagacaaat ggttcaagct gggcaacaac ctgaaccagc
     1801 gcgacaccat cgcggtgcgc cgcaccacct ctggcctctt gaagctggtg tgccccaacg
     1861 gcgaatacac caaagattca gtgcgcaagt gtctggaata cgcattggaa gcccgccgcc
     1921 gggtcaaaga gcagctcaag aagattggcg gcatggagtt ttatgacgtc catttcagtt
     1981 acatcgacct cgaagatggt gtcgagcgtt ttgtcaccgt gcccgagcag tccagcggtg
     2041 ccttggtgcc ggaaggtcgc ctgaaccccg gcactctgca taccatcagc atggggtcaa
     2101 ctgagatgcc tggcatctat cggctggaaa tccaatcgat tcctggcacc ggcaaagcca
     2161 atgtgtcagg cctggccccg cgtgaggcgg tgcgagtggc ttttgatgac ttcaaagcca
     2221 actcaagccg tgtcagtgcg tccatcaagc ctggtgagcg cgattttcat acgcatttag
     2281 tagagttgca gcactccggc gcacctcaat cctttacgct ggcgagtttc atcgccttct
     2341 gttcagcagc gcttggcaag ccggttcagt cgcaattggc tgtgatgggt gacatgagtt
     2401 tgggtggcac gattgtgcaa gtgcgtaacc tggctgaatg catgcaggtg gcgtttgatg
     2461 ccggagccaa gcgcatcctg ttgccgatgt ccagtgtgac cgctattgcc accgtgccgg
     2521 gtgaactgtt tgccaagttt caaaccagct tttactcaga cccggtggat gcggtgttca
     2581 aggcgctggg ggtgaagtag gtcatgaagt tcacccgtac cgccgacatc cattcgtgct
     2641 tttggtcatt gcctgcccca gcgcctggtg attctgcccc ttcacggtgg ctgttgcgca
     2701 acttggttcg ccgttggatg atcaaccaca atctggcaac cgtccgaact accccgatat
     2761 gaaacttgct cactctacat ccactctgcg gcaaattccg ccaggtgtct gggtgctggg
     2821 cttggtgagc atgctgatgg atatttcttc cgagatggtt catagcctgc tgcccatgtt
     2881 catggttacc gtgctgggga ccagcgcctt tacagttgga atcatcgaag gtctggcaga
     2941 gtcaaccgct ttgatcgtca aggtgttttc cggtgcgctg aacgactacc tgggaaaacg
     3001 caaaggcttg gccgtgatcg gctatgccct gggtgcattg accaaacctc tatttgccat
     3061 cgcatcagga accggcattg ttgttacggc ccggttgctc gatcgggtgg gcaaaggcat
     3121 tcgtggggca ccgcgagatg ccttggttgc agacatcacc cccagccata ttcgaggcgc
     3181 tgcttttggc ttgcggcagt cattggacac ggtgggtgcg ttcaccgggc cgctgattgc
     3241 tgtgggcttg atgctcctgt ggtctaacga ttttcgggcg attttctggg tggccgtggt
     3301 acccggacta ctcgccgtcg cgctgctgct gttcggtgtg catgagcctg agcgacatgt
     3361 aggggagccg acaagcaacc cgattcggcg cgaaaacctg agtcgtcttg gccggtccta
     3421 ctggtgggtg gtgaccattg gtgctgtgtt taccctggcc cgtttcagtg aggcgtttct
     3481 gatacttcgc gcccaacaaa gcggcgtgcc catggcgttg gtgcctttgg tgatggtggc
     3541 aatgaatctg gtttatgcg
//
LOCUS       DAOSLL010000516         3547 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_23095_length_3547_cov_3.917239, whole genome shotgun sequence.
ACCESSION   DAOSLL010000516 DAOSLL010000000
VERSION     DAOSLL010000516.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3547)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3547
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23095_length_3547_cov_3.917239"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="PLB25_18070"
     CDS             <1..383
                     /locus_tag="PLB25_18070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOE43513.1"
                     /translation="RLGRSLSHLLSIVTALKEKHVAFRSLTEGMDTTTASGELLFHVF
                     GALAQYERALIQERVIAGLGAAKRRGRIGGRPQAITGEKLDAIVAALDGGMSKAAVCR
                     NFGVKRTTLIETLARIAWPGAHET"
     gene            396..3449
                     /locus_tag="PLB25_18075"
     CDS             396..3449
                     /locus_tag="PLB25_18075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HOE43514.1"
                     /translation="MRNNPGKRLTILSDAEKVALYGLPDFDDFQRVEFLAMTDAERTL
                     ALRRKGFEEQIYCLLQIGYFKAKQAFFHFSLADASPNDIGFLLQRYFPGKELTSTSKP
                     LSKRQYYAQRDDISNLFGYRLCSEADLPSLLEKATLLARTDVAPTFLLAELMVFLVSQ
                     HIVRPGYSTLQELITNALTAERDRLEQLVESALTDATRAALQELLMGDNTLSDLAALK
                     QDAKSFRYRQMGLERQKRLTLAPLYTIAKTLLPSLDISQLNIAYYASLANFYTIYDLR
                     RFKPGQTNLYLLCYAWQRYRQLTDNIVEAFAYQARHLEDDTKAVATQQAAKIHNERQQ
                     ATPRVGELLLLYVDDSLTDMTPFGTVRGKAFRIMPEEKLRSTGKLLTETPVSQMDLRW
                     QAVDKQSGLCTKNLRPLATALDFASSSASGKVWLAALQWMNEVFAGHKRLATQPLEDI
                     PQRTIPTRLRNFLLSFDEEGKPVGLRADRYEFWVYRQLRKRLDAGDIYLDDSVQHRRF
                     ADDLVSMEAKADALKALNIPWLREPADQTLDALFVELDEQWRAFDSELRSGKLTHLEF
                     DPVKQTLTWHRPKADKDKQLQQGFYGKLQPRDVADVFRFVNERCNFLSAMTPLQPRYA
                     KKVADADSLMAVIIAQAMNHGNFKMAETCDIPYHVLEATHQQHLRPATLIEASDILSN
                     FIASLPIFPYYSIDMDVLYGSVDGQKFAAVSPTLKARHSRKYFGKDRGVVAYTLLANH
                     VALQTQMLGANQHESYWVFDICYNNTSDIKPTTITGDMHSINMANFAILHWFGMNLAP
                     RFTNLHAQLKHLYRGPDQEGYADFLIQPVGQIDRHLIAAEKSNLDHIAATLGLKEMSQ
                     SVLVRKICTLSGHHRTRKALFEYDKLIRTIYTLRYLRDPQLQRDVHRSENRIESYHQL
                     RSTIAQVNGKKELTGRTDLEVEISNQCGRLIANVVIAYNSMLLSGLLGRHLAVGNEKA
                     LDLLRRISPAAWQHLHFLGHYAFRDKRNPIDLEAVLAGIDWEATD"
BASE COUNT          802 a         1021 c          957 g          767 t
ORIGIN      
        1 atcgacttgg ccgttcgctg tcgcatctgc tctcaatcgt gaccgcgttg aaggaaaagc
       61 atgttgcctt tcggtcgctg acggaaggca tggacaccac tacggcgtcg ggcgaactgc
      121 tgtttcatgt cttcggtgca ctggctcagt acgaacgggc cttgattcag gaacgggtta
      181 ttgctggtct aggtgctgcc aaacggcgcg gccgtatagg cgggcggccc caagcaatta
      241 caggtgagaa gctggatgcc atcgtcgccg cgttggacgg tggcatgtca aaggccgccg
      301 tgtgccgcaa cttcggcgtt aaacgtacca cgttgatcga aacgttggcc cgtatcgcgt
      361 ggcccggtgc acacgagacc taaccggttt acaaaatgcg taataacccc ggtaagcgtc
      421 tgacgatact ctcagatgct gagaaagttg cactctacgg ccttccagat ttcgatgatt
      481 tccagcgtgt cgaatttctc gccatgactg atgccgagcg caccctggcc ttgcggcgca
      541 agggttttga agaacagatc tactgtctac tgcaaattgg atatttcaaa gccaagcagg
      601 cgtttttcca tttttctctg gctgatgcat caccaaacga catcggtttc ttacttcagc
      661 ggtatttccc aggaaaggaa ttgacatcaa cctccaagcc gctaagcaaa cggcaatatt
      721 acgcacagcg tgacgacatc tccaacctgt ttggctatcg actgtgctct gaagctgatc
      781 tgccttcgct acttgaaaag gcaacgctgc tggctaggac agacgtagca ccgacttttt
      841 tgctggcgga actgatggtg tttctggtta gccagcacat cgtgcgacca ggctactcca
      901 cgctacaaga attaatcaca aatgcactaa ctgctgagcg cgatcgcctg gaacaactgg
      961 tcgaatcggc actgaccgat gccacgcgcg ccgcgctgca agagttgctg atgggtgata
     1021 acactttgtc cgatttagcc gcgctcaagc aggacgccaa gagctttcgc tatcgccaga
     1081 tgggcttgga gcgccaaaag cgactcaccc tggcgcccct gtacacgatc gccaaaaccc
     1141 tgctgccaag ccttgatatc tcgcagctca acatcgccta ctacgccagt ctggccaact
     1201 tttacacgat ctacgattta cggcgattca agcccggcca gaccaacctc tatttgctgt
     1261 gctacgcctg gcagcgctac cggcaactca ccgacaacat cgtggaagcc tttgcttacc
     1321 aggcccgtca tcttgaagac gacaccaagg cggttgccac acagcaggcg gccaaaatac
     1381 acaacgaacg ccagcaagct acgccacggg tgggtgaact tctcctactg tatgtggatg
     1441 actcgctcac tgacatgacc cctttcggca cggtgcgcgg caaggccttt cgcatcatgc
     1501 ccgaggagaa gctgcgctcg actggcaagc tcctgacgga gactcctgtc agtcagatgg
     1561 atttgcgttg gcaagcggtg gacaagcaga gtgggctttg cacgaaaaat ctacgccccc
     1621 ttgccacggc cctggatttc gcaagcagta gtgccagtgg caaggtctgg ctggcggcct
     1681 tgcaatggat gaacgaggtc tttgccggcc acaagcggct tgccacgcag ccgttggagg
     1741 acattccgca gcgcacgata cccacccggt tgcgcaattt cttgctcagc tttgacgagg
     1801 agggcaagcc cgttggtctg cgcgccgacc gctacgagtt ttgggtgtac aggcaactgc
     1861 gcaagcgcct cgacgctggg gacatttacc tggacgacag tgtgcagcac cggcgttttg
     1921 ccgatgacct ggtgtccatg gaggccaaag ccgacgccct caaggcactg aatatccctt
     1981 ggttgcgtga gcctgccgac caaacacttg atgccttgtt cgtcgaactg gacgagcagt
     2041 ggcgggcgtt cgattctgag ctgcgcagcg gcaagctcac gcacctggag tttgacccag
     2101 taaaacaaac gttgacgtgg caccgaccca aggccgacaa ggataaacaa ttgcagcaag
     2161 gcttctacgg aaagcttcag ccgcgtgacg ttgccgacgt cttccggttt gtgaacgaac
     2221 ggtgcaactt tctctcggcc atgacgccct tgcagccacg ctacgccaag aaagtcgccg
     2281 acgctgacag cctgatggcc gtgatcattg cccaggccat gaaccatggc aacttcaaaa
     2341 tggctgagac ttgcgacatc ccttaccacg tgctggaagc cacacaccag cagcacctgc
     2401 ggccggccac gctgattgag gcgagcgaca tcctcagcaa cttcatcgcc agtctgccca
     2461 tcttcccgta ttactccatc gacatggatg ttctgtatgg cagtgtggac gggcagaaat
     2521 ttgccgctgt ctccccgaca ctcaaggcac ggcactcacg caaatatttt ggcaaagacc
     2581 gtggcgttgt cgcctatacc ttgctggcca atcatgttgc tctgcaaacc cagatgctgg
     2641 gcgccaacca gcatgagagc tactgggtgt ttgacatctg ctacaacaat acctccgaca
     2701 tcaagccgac cacaatcaca ggcgacatgc acagcatcaa catggcgaac tttgccatcc
     2761 tgcactggtt tggtatgaac ctggcgccac gttttaccaa tttgcatgcc caattgaagc
     2821 acctctaccg tggccctgac caggagggtt atgctgattt cctgattcaa ccggttggac
     2881 agattgaccg tcatttgatt gccgctgaaa aatctaattt ggatcacatc gctgccaccc
     2941 tgggactcaa ggaaatgagc caaagcgtgc tggtacgcaa gatttgcaca ttgtctggac
     3001 accaccgcac gcgtaaggcg ctcttcgagt atgacaagct gatccgaacg atctacacgc
     3061 tgcgctactt gcgcgaccct caattgcagc gtgatgtcca tcgctcggaa aaccgcattg
     3121 agtcctacca ccagctgcgc tccaccatcg cccaggtcaa cggaaagaag gaactgaccg
     3181 ggcgtacgga cctggaagtg gagatcagca accagtgtgg ccgactgatc gccaatgtgg
     3241 tgattgccta caactcgatg ctgctgtcag ggctacttgg ccggcatctg gcagtcggca
     3301 atgaaaaggc gctggactta ctcaggcgaa tctcacctgc ggcgtggcag cacctgcatt
     3361 tcctggggca ctatgctttc agagacaagc gcaatccgat tgatttggaa gctgttttgg
     3421 cggggattga ttgggaggcg acagactgat ttaggtcagc gggaaattcc gagggtttgg
     3481 gcttacaacc ccggaacggc tgttttatgc cgcttgcggc atgtagcgaa gcggtacggg
     3541 tgttcag
//
LOCUS       DAOSLL010000517         3537 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_23179_length_3537_cov_5.004595, whole genome shotgun sequence.
ACCESSION   DAOSLL010000517 DAOSLL010000000
VERSION     DAOSLL010000517.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23179_length_3537_cov_5.004595"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(18..1931)
                     /locus_tag="PLB25_18080"
     CDS             complement(18..1931)
                     /locus_tag="PLB25_18080"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HOE43515.1"
                     /translation="MDLDDALETFIVECRELLEDMETALLAVEDADDKKEMINAVFRA
                     AHTIKGSSGLFSMDHIVAFTHVVESLLDRMRAGKLEMSDGLVVLLLACCDHMGALVEG
                     VAAGEHAGSAELLQQGLPLVAQLRAYLDAPKTEALQAKSTLYVPAADTEKVQRIDGEG
                     VNADHWHISVRFGPDVLRNGMDPLSFIRYLGTLGKIIGIATLTDALPNAEQMDPEICY
                     LGFEIAFQSKADKAAIEKVFEFVRDDSRLLILPPHSLISEYVSLIGQQQGELSRLGDM
                     LVQCGSLSQQELDIALNSQVDHPTTPIGTILVQQGSVQPEVVEAALTKQKQVKETGVA
                     ESRSIRVDADKLDQLINLVGELIIAGASVNMIAHRAKVIELTESTSKLATLVEEVRDS
                     ALQLRMVRIGATFSRFQRVVHDVSRDLGKDIMLVIDGEETELDKTVVEKIGDPLMHLV
                     RNSMDHGIESADVRAARGKPAQGTLKLNAYHDSGAIVITVEDDGGGLKRERILAKALE
                     RGLIEPGQHLSDTDVYGLVFEPGFSTAEKVTNLSGRGVGLDVVKRNITALRGTVSINS
                     EEGVGTIVTVRLPLTLAIIDGFLVGVGKSVYAIPLDMIEECVAYSAEPGHDYTNLRGE
                     VLPVTIPRQSRGL"
     gene            complement(1933..2244)
                     /locus_tag="PLB25_18085"
     CDS             complement(1933..2244)
                     /locus_tag="PLB25_18085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019923536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HOE43516.1"
                     /translation="MNESNVLRIEGELTIFRAMELKPAMLPVPPLTEIDLSGVTDLDT
                     AGVQLLMLAKKTAQAEQRELRLTGHSPAVLEVFELLNVAAYFGDHLVMDSRLSTGAAR
                     S"
     gene            complement(2480..2848)
                     /locus_tag="PLB25_18090"
     CDS             complement(2480..2848)
                     /locus_tag="PLB25_18090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE43517.1"
                     /translation="MAKTIMIVDDSASMRRVVGIALKGAGYDVLEGCDGKDALGKLKG
                     QKVHMIISDVNMPVMDGIAFLKAVKQMPTYKFTPVIMLTTESAEEKKREGQAAGARAW
                     VVKPFQPEQLVSAVQRLCLP"
     gene            complement(2859..>3537)
                     /locus_tag="PLB25_18095"
     CDS             complement(2859..>3537)
                     /locus_tag="PLB25_18095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE43518.1"
                     /translation="GMLTKVQGLDRFIVELQEMASDVARIAQQTNLLALNAAIEAARA
                     GDLGRGFAVVAKEFRMLSNQSGETGRRIAEKVKIISAAIVDTCTVVRESVAAEDSSME
                     AAHATVDRVLTDFKGITEAFQNSSNLLQVESVSIQSEVNNALVQLQFQDRVSQIMNQV
                     IKNMELLPGVFEQQHAAYLQTKTLQPPNPQEMLGELKKTYVMADQHVIHDGGTVEQRN
                     TTDISFF"
BASE COUNT          678 a         1062 c         1039 g          758 t
ORIGIN      
        1 ttagcggctc acaattctta aagcccccgg ctttgccggg ggatggttac tggcagcact
       61 tcgccacgca ggttggtgta gtcgtgaccc ggctcggccg aataggccac gcactcttcg
      121 atcatgtcca gcggaatggc atagaccgat ttgccgaccc cgaccagaaa accgtcaatg
      181 atggccaggg tgagcggcag ccgcacggtg acgatggtgc cgacaccttc ttcgctgttg
      241 atgctaacgg tgccacgcag ggcagtaatg ttgcgcttga ccacatcgag cccgacacca
      301 cggccagaca ggttagtgac cttttcggcg gtagaaaaac cgggctcgaa caccagccca
      361 taaacatcgg tatcgctcag gtgctgaccc ggctcaatca ggccacgctc caatgccttg
      421 gccagaatgc gctcgcgctt gaggccaccg ccgtcgtctt caacggtgat cacgatggcc
      481 cccgagtcgt ggtaagcgtt gagcttgagc gtgccctgcg ccggtttgcc gcgtgcggca
      541 cgaacatctg ccgactcaat gccatggtcc atcgaattgc gcaccaggtg catcagcggg
      601 tcgccaattt tttcgaccac ggttttgtcg agttcggtct cttcgccgtc gatcaccagc
      661 atgatgtctt tgcccaggtc acgcgacacg tcgtgcacca cacgttgaaa gcggctgaag
      721 gtggcaccaa tgcgcaccat gcgcagttgc agcgcactgt cacgcacctc ttcgaccagg
      781 gtggcgagtt tggaggtgga ttcggtcagc tcgatgacct tggcgcggtg cgcaatcatg
      841 ttgacgctgg cgccggcaat gatgagttcg cccaccaggt tgatgagttg gtcgagcttg
      901 tcggcatcaa cgcggatgct gcggctctcg gcgacaccgg tttccttgac ctgcttttgc
      961 ttggtcagcg cggcttcgac cacttcgggt tgcaccgagc cctgctgaac cagaatggtg
     1021 ccaatcgggg tggtcggatg gtcaacctgc gagttcagcg caatgtcaag ttcttgctgg
     1081 ctcaagctgc cacattgcac cagcatgtcg cccaggcggc tgagttcgcc ttgttgttgg
     1141 ccgatcaggc tgacgtattc agaaatcagg ctgtgcggcg gcaggatcag caggcggctg
     1201 tcgtcgcgga caaactcaaa caccttttca atcgccgcct tgtcggcctt gctctgaaac
     1261 gcgatctcaa agcccaggta acagatttcg gggtccattt gctcggcatt gggcagggca
     1321 tcggtcaggg tggcaatacc gatgatcttg cccaaggtgc ccaggtagcg gatgaacgac
     1381 agcgggtcca tgccgttgcg cagcacatcc gggccaaaac gcaccgaaat atgccagtgg
     1441 tcagcgttga ccccttcgcc atcgatgcgc tgcacctttt cggtatcggc agcaggcaca
     1501 tacagggtcg atttggcctg cagtgcttct gtcttgggag cgtccaaata ggcgcgcaac
     1561 tgggccacca gcggcaggcc ttgctgcaac agttcggctg agccggcatg ttcgcccgcc
     1621 gccacaccct cgaccagcgc gcccatgtgg tcgcagcagg ccagcagcag caccactagc
     1681 ccgtctgaca tttcgagctt gcctgcgcgc atgcggtcca gcaggctttc gaccacatgg
     1741 gtaaaagcca caatatggtc catgctgaac aggcctgacg agcccttgat ggtgtgcgcg
     1801 gcacgaaaca ccgcgttgat catctctttt ttatcgtcgg catcttccac cgccagcaag
     1861 gcggtttcca tgtcttcaag cagttcacgg cattcaacaa tgaacgtctc cagtgcgtcg
     1921 tcaagatcca tgtcagctcc ttgcagcacc ggtggaaagg cgcgagtcca tcaccaggtg
     1981 atcaccaaaa taggccgcca cgttgagcag ctcaaacact tcgagcaccg ccgggctgtg
     2041 gccggtcagc cgcagctcgc gctgctcagc ctgtgcggtt tttttggcca gcatcagcag
     2101 ttgcacaccg gcggtgtcga gatcggtcac gcccgagagg tcaatttcgg tcaggggcgg
     2161 caccggtagc atggcgggct tgagttccat ggcgcgaaaa atggtcagct cgccttcaat
     2221 gcgcagcacg tttgattcgt tcatcctaga ttcctcggtt ggacgcgccc gagtgggccg
     2281 ctggcagcgc gtctgggtag gcgcgacgac gacgcgggct actgcccgct aggaggagca
     2341 acgcccccag gcgcgttgcc agtggttcaa tcgggcgcgt agcgccgtca ctcaacgggt
     2401 gaactccatc gaagctaaaa aagtaaatgc tcataaggac ttacaggtgg aaacaagcgg
     2461 tcaacggagg aatctaggat catggcaggc acagccgctg cacggcgctc acgagttgtt
     2521 cgggctgaaa cggtttgaca acccaggcgc gtgcaccggc tgcctggcct tcacgctttt
     2581 tctcttcggc cgactcggtc gtcagcatga tgaccggggt gaatttgtag gtcggcatct
     2641 gcttgaccgc tttcaaaaag gcgatgccgt ccatcaccgg catgttgaca tcgctgatga
     2701 tcatgtgcac cttttgcccc ttgagcttgc cgagcgcgtc cttgccgtcg cagccttcaa
     2761 gcacgtcgta tcccgcgccc ttgagggcaa tgccgacaac ccgtcgcatg gatgcagagt
     2821 cgtcaacgat catgatggtc ttggccatgg ctattcctct agaaaaaact gatatcggtg
     2881 gtgttgcgtt gctcaaccgt gccgccgtcg tggatcacat gctgatcggc catgacgtag
     2941 gtctttttca gctcgccgag catctcttgc gggttgggcg gctgcaaggt tttggtttgc
     3001 aggtaggcag cgtgctgctg ctcaaacacg cctggcaaca gctccatgtt cttgatcacc
     3061 tggttcatga tctggctgac ccggtcctga aactgcaact gaaccagcgc gttgttgact
     3121 tcggactgga tgctgacact ctcgacctgc agcaggttgc tggagttctg aaaagcctcg
     3181 gtgatgccct tgaagtcggt caatacccgg tcaaccgtgg cgtgcgccgc ttccatcgag
     3241 ctgtcctcgg ccgccaccga ttcacgcacc acggtgcagg tatcgacaat ggctgcgctg
     3301 atgatcttga ccttttcggc aatgcgccga ccggtttcgc cagactggtt ggacagcatg
     3361 cggaactctt tggccaccac cgcaaagccg cgcccgaggt caccggcgcg cgctgcttca
     3421 atggcagcat tgagtgccag cagattggtc tgctgggcaa tgcgcgccac gtcgctggcc
     3481 atctcctgca gttcgacaat aaagcggtcc aggccttgca ccttggtgag catgcct
//
LOCUS       DAOSLL010000518         3536 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_23189_length_3536_cov_3.478311, whole genome shotgun sequence.
ACCESSION   DAOSLL010000518 DAOSLL010000000
VERSION     DAOSLL010000518.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23189_length_3536_cov_3.478311"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..597
                     /locus_tag="PLB25_18100"
     CDS             187..597
                     /locus_tag="PLB25_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43519.1"
                     /translation="MRLLLVMILIALLPLRGWTASAMLADHEWPSVTSKTVAASTYST
                     RATGIFYAQTRPLPANCPEHAAQSTTAADQDHTPTRSNTSAACDACGLCHLSVATCDL
                     LPVPEVTALATLPTAIASLFASVITPPGLKPPIA"
     gene            877..2388
                     /locus_tag="PLB25_18105"
     CDS             877..2388
                     /locus_tag="PLB25_18105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004537959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HOE43520.1"
                     /translation="MQKRTFLTTLASLPLLGLKQSWAQAIGGMDHSQHGMATSAKATQ
                     AATALAPVSALPAGAPLATLVKLANTSKAPGVFQATLTAAPVTLPLIAAGPTELWAYN
                     ASLPGPLIEVFEGDTVEILFINRLPQPSTIHWHGLPVPSEQDGNPQDAVAPGAQRIYK
                     FTLPKGCAGTYWYHPHPHGFTAEQVYRGLAGTFIVRAKDDPLRHLPERLLVCSDLKLS
                     ADGRIAPSDANDEMDGREGQFALVNGQRQPLLAFDAGGRERWRIWNANSARYLRLRLP
                     GATLTLVGTDGGLLEQPVSGLTELLLAPAQRIELIVDAPAKAMRVALVADTYARGKMG
                     DAPPDQPVSLLQVDFAARSGQALAALPAKLRAIADPGPVQAKKRVVFSEKMSMAGGVH
                     SMAFLVNGKQFDMGRFDLVSRVGEVELWEVANESDMDHPFHIHGTQFQVVESELAGKI
                     TPAAYRAWRDTVNLKSGETVRLKLVQHFKGPRMYHCHILEHENAGMMGQLNVV"
     gene            2736..3431
                     /locus_tag="PLB25_18110"
     CDS             2736..3431
                     /locus_tag="PLB25_18110"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE43521.1"
                     /translation="MKTVLITGANRGIGLEHAKVFSARGIRVFATARSPSEATELAAL
                     SQASGGNLKVLPYDAASPDAPALLKQALGETPIDLLLANAGGMGNRDVAFGSVDVEEV
                     LQLVRINSLAPLKLAESLAENLTCSQWKLIAFQSSLMGSIGDNGSGGYYAYRISKVAL
                     NMVARSVANDLKSRGVIAVALHPGWVKTRMGGSNAPITVAQCVDGQQRLFDKLTLADT
                     GKFFNFDGKVLPW"
BASE COUNT          716 a         1128 c         1042 g          650 t
ORIGIN      
        1 agtcgctttt gaatgctagc cgcgctcgct gaggtggctt cgggcatcac ccgcccaatt
       61 tcagccatcc cggcaacgcc tgtttgccct gccggcaagc catagcgcgg gattttgcgc
      121 tgggaaactt tgatgccttg accgtttggc gcggaacttt gccaggcccg ctggcctcct
      181 aaaccaatgc gtttactgct tgtcatgatc ttgatcgccc tgctgccctt gcgtggctgg
      241 accgcaagcg ccatgttggc cgaccatgag tggccctcgg tcacatcaaa aacagtagct
      301 gccagcacct acagcacaag ggctacaggc attttttatg cccaaacacg gccgctgcca
      361 gcaaattgtc cagagcacgc agcccagtcc actaccgcag ccgatcagga ccacaccccc
      421 acgcgcagca acaccagcgc cgcttgtgat gcctgcgggc tttgccacct gagcgtggcc
      481 acctgcgacc tgctgccagt gcctgaagtc acggctttgg ccaccctgcc gacagcaatc
      541 gccagcctct ttgccagcgt gataacgccc ccagggctca aaccacccat cgcctgacca
      601 acgggcaagc tgcggcaaca cagcgacttg gcacagctcg cagccggtca agcgcgccaa
      661 aatgcactct gccatccagt accgcccaaa cccgctcacg ctggccttgt ctgagcgggt
      721 caatgggtta acagcctggc attgcgccgg gcattcaatt cttgccgcct gctgccggtg
      781 cgacccagca tgctatataa caaatagctg cctgcgcata tacagcaagg gctacaggca
      841 tatttcgcac ttattcaaag caacaggcag aacatcatgc aaaaacgaac cttcctcaca
      901 accctggcca gcctgccctt gctgggcttg aagcaaagct gggcccaagc catcggcggc
      961 atggatcaca gccaacacgg catggccacc tctgccaagg ccacccaggc cgccacagcg
     1021 ttggcacccg tgagcgcgct gcctgcgggc gcaccgctgg ccacgctggt caaactggcc
     1081 aacaccagca aggcacctgg cgtgtttcaa gccaccctga ccgccgcacc ggtcaccctg
     1141 cccctgattg ccgctggccc gaccgagctg tgggcttaca acgccagcct gccgggcccg
     1201 ctgatcgagg tgtttgaagg tgacacggtc gaaatcctgt tcatcaaccg cctgcctcag
     1261 cccagcacga tccactggca tggcctgccg gtgcccagcg agcaagacgg caacccgcaa
     1321 gacgcggtgg cccccggcgc gcagcgcatc tacaagttca ccctgcccaa gggttgcgcg
     1381 ggcacttact ggtatcaccc gcacccgcat ggctttaccg ccgagcaggt gtaccgtggg
     1441 ctggcaggca ctttcatcgt gcgtgccaag gacgacccgc tgcgccactt gcccgagcgc
     1501 ctgctggtgt gctcagacct caagctcagc gccgatggcc gcattgcccc aagcgatgcc
     1561 aatgacgaga tggacggtcg cgaaggccag tttgcgctgg taaacggcca gcgccagccg
     1621 ctgctggcct ttgacgcggg tggccgcgag cgctggcgca tctggaacgc caacagcgcc
     1681 cgctacctgc gcctgcgcct gccgggtgcc acgctgaccc tggtgggcac cgatggtggt
     1741 ctgctggaac agccggtaag cggcctgacc gagctgctgc tggccccggc gcaacgcatc
     1801 gaactgatcg ttgacgcacc cgccaaggca atgcgtgtgg ccttggttgc cgacacctac
     1861 gcacgcggca agatgggcga tgcgccgccg gaccaaccag tgagcctgct gcaagtggac
     1921 tttgcagcgc gcagcgggca ggcgttggca gcactcccgg ccaaattgcg cgccatcgcc
     1981 gacccggggc cagtgcaggc caaaaagcgc gtggtgttct ctgaaaaaat gagcatggcc
     2041 ggtggtgttc acagcatggc gtttctggtc aacggcaagc agtttgacat gggccggttc
     2101 gatctggtca gccgcgtcgg cgaggtggaa ctgtgggaag tcgcgaacga atccgacatg
     2161 gaccacccgt ttcacatcca cggcacccag tttcaggtgg tcgagtccga actggcgggc
     2221 aaaatcaccc ccgcagccta ccgggcctgg cgcgacaccg tcaacctgaa gtctggcgag
     2281 acggtgcgtc tcaagctggt gcagcatttc aaggggccac gcatgtacca ctgccacatc
     2341 ctggagcacg aaaacgccgg gatgatgggg caattgaacg tggtttgaca agcggcaggg
     2401 gcagcaaggt gacagccgcg ccgccagcgg cccgctcggg cgcgtccaac ttagtctctg
     2461 acaagtcaag tctttgcaca tcatgcgaag aattcaaaca ttcaaagtca ttcgtcgcga
     2521 gggcgcgact cctacaaatt cagataggca ccgactgttt gtgcaagagt cgcgcccccg
     2581 ggacggaggc tcatcgattg cggctcgtcc ggcgtaggaa gtaggaactt aggaacttag
     2641 gaaattagga acttaggttc aaatccggca cgccagatgt cccaggccat ttggctacca
     2701 tctgagccta tttttcacct tatggacaaa atcaaatgaa gacagtgctg atcacgggag
     2761 ccaatcgcgg cattggtctt gaacacgcaa aggtattttc agcgcgcggc atccgcgtgt
     2821 ttgccacggc ccggtcgccg tcagaagcca ccgagcttgc cgcactgtcg caggcaagtg
     2881 gcggcaactt gaaggtgctg ccgtacgacg ccgccagtcc agatgccccg gcactgttga
     2941 agcaggctct gggtgagaca ccgattgacc tgttgcttgc caacgccggg ggcatgggca
     3001 accgggacgt ggcgtttggg tctgttgatg ttgaagaggt gctgcagctt gtccgcatca
     3061 actcacttgc accgctgaag ctggcggaaa gtttggccga gaacctgacc tgttcgcagt
     3121 ggaagctgat cgctttccaa tcgagcctga tgggatcaat cggcgacaac ggttcgggcg
     3181 gctattacgc ctaccgcatc tccaaggtgg cactgaacat ggttgccagg agcgtcgcaa
     3241 atgatctgaa atcgcgtggg gtgatcgcgg tggcgctgca tccggggtgg gtcaagacgc
     3301 gcatgggtgg ctcaaatgcg ccaatcaccg tggcgcaatg tgttgacggg cagcagcgtt
     3361 tgtttgacaa attgacactg gctgacaccg ggaaattttt caacttcgat ggcaaggtgc
     3421 ttccctggtg aagtcattcg agctggccgc gctgccagct tcgcagacgc gagcatcagc
     3481 cgctcatcgc caccaccatc gccttgccgc tctgggtggt gttgcgctgc tcaaag
//
LOCUS       DAOSLL010000519         3535 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_23195_length_3535_cov_5.156322, whole genome shotgun sequence.
ACCESSION   DAOSLL010000519 DAOSLL010000000
VERSION     DAOSLL010000519.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23195_length_3535_cov_5.156322"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1001
                     /locus_tag="PLB25_18115"
     CDS             <1..1001
                     /locus_tag="PLB25_18115"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HOE43522.1"
                     /translation="EAAEQARVVGEKVLSSMNQSYHLAGFAHHSTPSIGITLFSGHQE
                     NTDDLLKRADLAMYQAKAAGRNTLCFFDPEMQSVVAARASLEADLRNAILQSQFVLFY
                     QAQMDNQAGLTGAEVLLRWQHPERSMVSPAEFIPLAEQSGLILPLGHWVLETACVQLA
                     LWAVQDKLTHLSISVNVSARQFRLPDFVDQVRSILDHTGARPQRLKLELTESMLVSDV
                     EDIIVKMTALKDIGVSFSLDDFGTGFSSLSYLKRLPLDQLKIDQGFVKNILTDPNDAA
                     IAKMVIALAQSMGLSVIAEGVELETQRDFLDRLGCHAYQGYLFSRPLPLQEFLKLV"
     gene            complement(1369..>3535)
                     /locus_tag="PLB25_18120"
     CDS             complement(1369..>3535)
                     /locus_tag="PLB25_18120"
                     /inference="COORDINATES: protein motif:HMM:NF012862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HOE43523.1"
                     /translation="GRYGLRRWSDAGRGTARDFSIAGNSVVSLRPLADGRVLFAATDA
                     SWGWLPGQAVPGTGVDGADGARVFSSGLTDFRGRRADFRVARDGSAFSYGFRNAAGAT
                     QAGAFDVARAQWTGNQPEWTPAQASGRGVLLEDWFESAQPRLNGRLLALTDNELAMSA
                     AVATDSSGFALGTSAYLRYYRADGSEQWRMAVPATTWQVNVSGDGRWVMAAFADGTLR
                     WYRSRDGLEMLALLLHADARRWVAWTAPGYYSASPGGEDLFGWQLDRGLTRAADFFPG
                     SRFRVRYFRPELIAQVLASADFETLQQSAAARPAGSAAPAPTAPGAGIEQQTPPVITL
                     LAPLDGDGFSQTEVTLRINMSSPAGAPPTRLRARLNGSLYELPGAGALADTAQRNPAT
                     DPTQPQELRYEQRIRLPASDAELMLFAENRNGYSTPAVLRLRWNGASRSEGTAPAPAV
                     APMSAATVMPAAAAMPDAADLRPVLYVLAVGVSKYQNKNIQLDFAAKDAADFANIFKL
                     QEKLLYRKVEVKLLTDATARRDDILDGLEWIRRMMTARDVGVVFLAGHGVNDNDGVYY
                     YLPQDTEVDKLKRSAVIFTEIKNTLAALPGKALFFIDTCHSGNVLGTGRRSIGKDLTA
                     VVNELSSAENGVIVFAAATGRQEAQESSEWGNGAFTRAVIEGMSGKADLARSGRITHQ
                     MLSLYVSERVKVMTQGAQSPTTIVPQGIADFPVAVSTSR"
BASE COUNT          704 a         1114 c         1059 g          658 t
ORIGIN      
        1 gcgaggcggc agaacaggcc cgtgtggtcg gtgaaaaagt gctctccagc atgaaccaga
       61 gctatcacct ggctggcttt gcccaccaca gcacaccgag tattggcatc accctgtttt
      121 ccgggcacca ggaaaacacc gacgatttgc tcaaacgtgc tgacctggcg atgtaccagg
      181 ccaaggcggc gggccgcaac accctgtgct tttttgatcc cgaaatgcag tccgtggtgg
      241 cggcacgggc atccctggaa gcggacttgc gcaacgccat cttgcagtcg cagtttgtct
      301 tgttctacca ggcacagatg gacaaccagg ccggtttgac cggggcagaa gtgctgctgc
      361 gctggcaaca ccccgaacgc agcatggtgt cgccagccga attcatcccg ttggccgaac
      421 agtccggcct gatcctgcca ctgggtcact gggtgctgga aaccgcctgc gtgcaactgg
      481 cgctgtgggc ggttcaagac aagctgacac acctgagcat ctcggtcaac gtcagtgccc
      541 gccaattcag gctgccggac tttgttgacc aggtgaggtc aatcctggac cacaccggtg
      601 cccggccgca gcggctcaaa ctggagttga ccgagagcat gctggtcagc gatgtcgagg
      661 acatcattgt caaaatgacc gccctgaagg atattggcgt gagcttctcg ctggacgact
      721 ttggcaccgg tttctcgtcg ctgtcttact tgaagcgtct gccgctggac cagctcaaga
      781 tcgaccaggg ctttgtcaaa aacatcctga ccgacccgaa cgatgcggcg attgccaaaa
      841 tggtcatagc gctggcccaa agcatgggcc tgtcggtgat cgccgagggg gtcgaactcg
      901 aaacccagcg ggactttctt gaccggctgg gttgccatgc ctatcagggc tatctgttta
      961 gccgaccgct gccgttgcag gagtttctga agctggtgta agcatgggtc catgccgcaa
     1021 atgcagttgc cgcctgctac cccaaagaaa tcagtccagg ttggcgccag atctcatccg
     1081 atatcaaggt gccaccgatg ccgtgattgc gcggccgggt cgatgctgcg tcaccgcccg
     1141 tttggcacaa gcaccgtgtc cagatgcaat tggactcaga ccccgatagc acctgccagc
     1201 actctgcaaa caatcaaaaa caatagctat cagcgcatat tccacaaggg ctggcggtca
     1261 atttcttttg aaatttttca gatgactccg gcagtcagtg cggtcagcag atcagtcgta
     1321 ttgcggcggc ctcatcgatc tgcatggctt gccagatgcc ggggaaaatt accgtgaagt
     1381 ggaaacggcc accgggaaat cagcgatacc ttgcggcacg atggtggtgg gtgattgggc
     1441 accctgggtc atcaccttga cacgctccga aacgtagagg ctgagcatct ggtgcgtgat
     1501 gcgaccgctg cgcgccaagt cggctttgcc actcatgccc tcaatcaccg cgcgcgtgaa
     1561 ggcaccgttg ccccactcgg atgactcctg cgcctcttgc cgaccggtgg ctgcggcaaa
     1621 aacaatcaca ccgttttcgg cactggagag ctcattgacc acggcggtca ggtccttgcc
     1681 gatggatcgc ctgccagtgc cgagcacatt gccagaatgg caggtgtcga tgaaaaacag
     1741 cgccttgccc ggcaatgccg ccagggtgtt cttgatctcg gtaaagatga cggcgctgcg
     1801 cttgagctta tcgacctcgg tgtcctgcgg caggtagtag taaacgccgt cgttgtcatt
     1861 gacaccatga ccggccaaaa aaaccacgcc aacatcgcgc gctgtcatca tccgcctgat
     1921 ccactccagc ccgtcgagaa tatcgtcacg ccgggctgtg gcatcggtca gcaatttcac
     1981 ctctaccttg cggtaaagca gtttttcctg caacttgaag atgttggcaa agtcggcggc
     2041 atccttggct gcaaagtcaa gctggatgtt cttgttctgg tatttgctga caccgacggc
     2101 cagcacgtaa agcaccggcc gcaagtcagc ggcatctggc atggccgcag ccgctggcat
     2161 gaccgtagcc gccgacatgg gtgcaacggc tggcgctggt gctgtgcctt cgctgcggct
     2221 ggccccattc cagcgcagcc gcagcacagc cggcgttgaa tagccattgc ggttttcggc
     2281 aaacagcatc agttcggcat ccgaggcagg caggcggatg cgctgctcgt agcgcaattc
     2341 ctgcggctgc gtcgggtctg tggccggatt gcgctgtgcc gtgtcggcca gggcaccagc
     2401 gcccggcaat tcatacaggc tgccattgag ccgcgcccgc agcctggttg gcggtgcacc
     2461 cgccgggctg ctcatgttga tgcgcagggt aacttcggtt tggctaaaac catcgccatc
     2521 cagtggtgcc agcagcgtga tgaccggtgg ggtttgctgc tcaatgccgg cacctggcgc
     2581 ggtcggcgcg ggcgccgcag aaccagccgg tcgggctgct gccgattgct gcagcgtctc
     2641 gaagtcggca ctggccaaca cctgggcgat cagttccggg cggaaatagc gcacgcgaaa
     2701 gcgcgaacca ggaaaaaaat cagcggctcg cgtcagcccc cggtccaatt gccagccaaa
     2761 caggtcttca ccgcccggac tggcgctgta gtagccgggc gcagtccagg ccacccagcg
     2821 ccgggcatcg gcgtgcagca gcaaggccag catttccagt ccatcgcggc tgcggtacca
     2881 gcgcagcgtc ccgtcggcaa aagccgccat gacccagcgc ccgtcgcccg agacattgac
     2941 ctgccaggtg gtcgcgggca ctgccatgcg ccactgctcg ctgccgtctg cacggtaata
     3001 acgcaggtag gcactggtgc ccaacgcaaa gccggaactg tcggtggcga cggctgccga
     3061 catggccagc tcgttgtcag tcagcgccag caagcggcca ttgagtctgg gctgtgcgct
     3121 ctcaaaccag tcttccagca aaacgccccg gccgctggcc tgggctggtg tccactcagg
     3181 ctggttgcca gtccactggg cgcgggcgac atcaaacgcg cccgcctggg tggccccggc
     3241 cgcattgcga aaaccatatg agaaagcact gccgtcccgg gcaacccgaa aatcggcgcg
     3301 acgaccgcga aaatcggtca ggccactgct gaaaacacgc gccccatctg cgccatccac
     3361 gccggtgccc ggcactgcct ggccaggcaa ccaaccccaa ctggcatcgg tggcggcaaa
     3421 cagcacccgc ccgtcagcca gcgggcgcaa gctgaccaca ctgttgccag caatgctgaa
     3481 gtccctggcg gtgccacgtc cggcatctga ccagcgccgc aagccgtagc gcccg
//
LOCUS       DAOSLL010000520         3528 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_23247_length_3528_cov_3.904405, whole genome shotgun sequence.
ACCESSION   DAOSLL010000520 DAOSLL010000000
VERSION     DAOSLL010000520.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23247_length_3528_cov_3.904405"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2062)
                     /locus_tag="PLB25_18125"
     CDS             complement(<1..2062)
                     /locus_tag="PLB25_18125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HOE43524.1"
                     /translation="MSRFNVKKVAVLGAGVMGAQIAAHLANVKVPVILFDLPAKEGQK
                     NGIVSRAVDGLKKLKPSPLGVAEDAALIGQANYEDHLELLKGCDLIIEAIAERMDWKL
                     DLYKKIAPFIAPHAIVASNTSGLSITKLSEALPEEIKPRFCGIHFFNPPRYMLLVELI
                     NTPTTQPQILDDLEAFVTSTLGKGVVRAKDTPNFIANRVGVAGMLATMKEVENFDLSY
                     DVVDDLTGKKLGRASSGTFRTADVVGLDTLAHVIKTLQDTLSLETDPFYASFATPAVL
                     KTLIEMGNLGQKSKAGFFKKAGRDILRFDLASKDYLPGGQKADEVYARMLKKPAAERL
                     QLLRNSEGAQGQFLWAILRNAFHYAAVHLAEVADNARDVDFCMRWGFGMKQGPFELWQ
                     EAGWLAVANMVKEDIDAGRALCQAPLPDWVFKGPVADAGGVHTPAGSWNPTAGQFVPP
                     RTLPVYERQFFPESVLGSNAPSAATAGKTLHEDAAIRLWTQDDDIVIASIKTKMHAIS
                     PGVVEGLVRAVDLAEASYKGVVIWSNDEMFSAGADLQGMLPAFMAGGVKAIDGAEAEM
                     QEAMLKIRYANVPVVSAVRGLALGGGCELAVYSAKRVVAMESYIGLVEVGVGLVPGAG
                     GLTYIARRAAENAASSTGKDLLPFLIEGFTAAAMAKVGTSALESRKLGYLLESDVIVA
                     HKDEL"
     gene            complement(2153..2611)
                     /locus_tag="PLB25_18130"
     CDS             complement(2153..2611)
                     /locus_tag="PLB25_18130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HOE43525.1"
                     /translation="MKNWTLALTGRALLATVFASLSAVAAAQMTPVGQWHSSDEKTGE
                     LKSLIIISETGGVLNGHVDKLLRKTADQKAVCKECTDDRKDKPMLGLQIIRGAKKVDG
                     KDVWEGGKILDPENGKEYTLRMTPIEDGKKLEVRGSILFVGRTQTWVRVQ"
     gene            complement(2644..>3528)
                     /locus_tag="PLB25_18135"
     CDS             complement(2644..>3528)
                     /locus_tag="PLB25_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019656891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOE43526.1"
                     /translation="SLGLTEVAFQNALAYAKDRIQMRSLTGPKAKDLPADPIIVHPDV
                     RKMLLTAKAYAEGGRALLCFSSMLLEKDHNHPDAAVRKDAGEMLAMLTPIVKAFITDN
                     GHLCTNACMQVFGGHGFIKENGMEQFVRDNRINMIYEGTNTIQSLDLLGRKVLGNQGA
                     TLKKFGKLIGALVAEEGVNEKMAEFITPIAILGDQMTKFTTELGFKGFQNPDEMGAAA
                     VDYLRVAGHLVFGYFWARMAQVALRQIAAGSTDPFYTAKLQTARFYFAKLFPETSTLM
                     RTARTGCKVLMDTDAALA"
BASE COUNT          687 a         1055 c         1018 g          768 t
ORIGIN      
        1 gcagttcgtc cttgtgcgca acgatcacat cgctttccag caagtaccca agcttgcgcg
       61 actcaagggc gctggtgcca accttggcca tggcggcggc agtaaagcct tcgatgagga
      121 acggcagcaa gtccttgcca gtcgaagatg ctgcgttttc tgcagcacgc cgcgcaatgt
      181 aggtaagccc gccagcccca gggaccagtc cgacaccgac ttcaaccagg ccgatgtaac
      241 tctccatcgc gaccacccgt ttggccgagt agaccgccag ctcgcagcca ccaccgagtg
      301 ccagaccgcg cacggcagac accaccggca cattggcgta acgaattttc agcatcgcct
      361 cctgcatctc ggcttcggcc ccgtcaatgg ccttgacacc accggccatg aaggctggca
      421 gcatgccttg caggtcggct ccggcagaaa acatctcgtc gttggaccag atcaccacac
      481 ccttgtagct ggcctcggcc aaatccacgg cccgcaccaa gccttcgacc acaccggggc
      541 tgatggcgtg catcttggtc ttgatgctgg caatcacgat gtcatcgtct tgggtccaca
      601 gccggatggc tgcgtcttca tgcagggttt tgccagccgt ggctgccgaa ggcgcattgg
      661 aaccgagcac actctcagga aaaaactggc gctcataaac cggcagggtt cgaggcggca
      721 caaattgacc cgccgttggg ttccaggaac ctgcgggcgt atgcacgcca ccggcatcgg
      781 ctaccggtcc cttgaacacc cagtctggca gcggtgcctg gcatagcgcc cgaccggcat
      841 caatgtcttc tttcaccata ttggctacag ccaaccagcc ggcttcctgc cagagttcaa
      901 acgggccctg tttcatgcca aagccccagc gcatgcagaa atcgacatca cgcgcgttgt
      961 cggccacttc ggccaggtgc accgcagcgt agtggaaggc gttgcgcaga atcgcccaca
     1021 aaaactgccc ctgtgcgccc tcgctgttgc gcagcagttg cagtcgctcg gcagcgggct
     1081 ttttcagcat gcgggcatag acctcgtcag ccttttgacc acccggcaga tagtctttgc
     1141 tggccagatc aaaacgcaaa atgtcgcgac ctgccttctt gaagaaaccg gccttgctct
     1201 tttggcccag gttgcccatt tcaatcaggg tcttgagcac ggctggtgtg gcaaagctgg
     1261 cgtaaaacgg atcggtctcc aggctcaagg tgtcctgcag cgttttgatc acatgggcca
     1321 gggtgtcgag cccgaccacg tcagcggtgc ggaaggtgcc tgaactggcc cgaccgagtt
     1381 ttttgccggt cagatcatcg accacgtcgt aactcaggtc gaagttttcc acttctttca
     1441 tcgtcgccag catgccagct acaccgacgc ggttggcaat gaagttgggc gtgtctttgg
     1501 cgcgcaccac gcctttgccc agggtgctgg tgacaaaggc ttcgaggtcg tccaggattt
     1561 gcggttgggt ggtcggcgtg ttgatcagtt ccaccagcag catgtagcgc ggcgggttga
     1621 agaaatggat gccgcaaaaa cgtggcttga tttcttcggg cagcgcttcg ctgagcttgg
     1681 tgatcgacag gcccgaggtg ttggaggcca cgatggcatg cggcgcgatg aacggcgcaa
     1741 tcttcttgta caggtcaagc ttccagtcca tgcgctcggc aatcgcttcg atgatcaggt
     1801 cacaaccctt gagcagttcc aggtggtctt cgtagttggc ctggccaatc agcgctgcat
     1861 cctcggcaac acccagcggc gacggtttga gctttttcag gccatccacc gcacggctga
     1921 cgatgccgtt tttttgcccc tctttggcgg gcaaatcaaa caggatgacc ggcactttga
     1981 cattggccag gtgcgccgca atttgcgccc ccatgacccc tgcgccgagg acggcgacct
     2041 ttttcacatt gaatcgagac atgtttttac tccgttcggg cttaagcttg tcgaagccct
     2101 tgaatacgtt ctgatgcgcc cttggacaag ctcagggcga acggtggcag gcttactgaa
     2161 cacgaaccca ggtctgggtt cgaccgacaa agaggatcga gccgcgcact tcgagctttt
     2221 tgccgtcttc aatcggggtc atgcgcaagg tgtattcctt gccgttttcg gggtccagaa
     2281 tcttgccgcc ttcccagaca tccttgccgt caaccttctt ggcaccgcgg atgatctgca
     2341 gccccagcat tggcttgtcc ttgcggtcat cggtgcactc cttgcacacc gctttctggt
     2401 cggcggtctt gcgcagcagt ttgtcaacgt ggccgttcaa gacaccgccg gtttcgctga
     2461 tgatgatcag gctcttgagc tcgccggttt tttcatcgct gctgtgccac tgacccacgg
     2521 gtgtcatctg cgccgcagca acagcgctga gtgacgcaaa aacagtagct agcagcgcac
     2581 gtccagtaag ggctagagtc caatttttca tacaaagtct cctgtaatca atgggtgaaa
     2641 aaatcaggcc agggctgcat cggtatccat caggaccttg cagccagtgc gtgcggtacg
     2701 catcagcgtc gaggtttcgg ggaacagctt ggcgaagtaa aaacgcgccg tttgcagctt
     2761 ggcggtgtag aacgggtcgg tgctgccagc ggcaatctgg cgcaaagcca cctgcgccat
     2821 gcgggcccag aagtagccaa acaccagatg cccggcgacg cgcaaatagt caacagcggc
     2881 agcgcccatc tcgtcggggt tctggaagcc cttgaaaccc agttcggtgg tgaacttggt
     2941 catctgatcg cccagaatgg caatcggggt gatgaattcg gccatttttt cattgacacc
     3001 ttcttctgcg accagcgcgc caatcagctt gccaaatttt ttcagggtgg cgccctggtt
     3061 gcccaagacc ttgcgcccca gcaggtccag gctctggatc gtgttggtgc cttcatagat
     3121 catgttgatg cggttgtcgc ggacaaactg ctccatgccg ttttccttga tgaatccgtg
     3181 gccgccaaaa acctgcatgc aggcattggt gcagaggtga ccgttgtcgg tgatgaaggc
     3241 tttgacaatc ggggtcagca tggccagcat ttcgccggca tccttgcgca ccgccgcatc
     3301 ggggtggttg tgatcttttt ccagcaacat cgaactgaag catagcaagg cacggccgcc
     3361 ctcggcgtac gccttggcgg tcaagagcat cttgcgcaca tcggggtgca cgatgatcgg
     3421 gtcggccggc aggtctttgg ctttggggcc tgtgaggctg cgcatctgga tgcggtcctt
     3481 ggcgtaggcc agggcattct gaaaggccac ctcggtcagg cccaggga
//
LOCUS       DAOSLL010000521         3521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_23306_length_3521_cov_4.049913, whole genome shotgun sequence.
ACCESSION   DAOSLL010000521 DAOSLL010000000
VERSION     DAOSLL010000521.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23306_length_3521_cov_4.049913"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..591)
                     /locus_tag="PLB25_18140"
                     /pseudo
     CDS             complement(1..591)
                     /locus_tag="PLB25_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465148.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
     gene            complement(767..1216)
                     /locus_tag="PLB25_18145"
     CDS             complement(767..1216)
                     /locus_tag="PLB25_18145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43527.1"
                     /translation="MKRPAAQAVISSFHPTARISVTTQNGCIVSGAKVYGSVLFTKVR
                     VAAYSLIEDSVLLPDVVVGRNVILKRVVVDNGCFIPDGFQVGVNPDADRARLHVTDLG
                     ITLITPECSVSRLIRCCNPTCGPSQQWVPAQQRRVLPRRVCKNGSQA"
     gene            complement(1221..1481)
                     /locus_tag="PLB25_18150"
     CDS             complement(1221..1481)
                     /locus_tag="PLB25_18150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43528.1"
                     /translation="MPDDSLPANTVPAASIADVSHLAAEDRYRVLVELVPFHLLETGL
                     DGTILWANQAGLQFVGVSDVHAIAGVTFASIVGDSNQAQVNR"
     gene            complement(2067..3251)
                     /locus_tag="PLB25_18155"
     CDS             complement(2067..3251)
                     /locus_tag="PLB25_18155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43529.1"
                     /translation="MGEPQQLKLEAIEALISARNVNDLSIREILEDILDDAQMAAFLP
                     DSLCQIDPRTGDVIVYNSSRATRLHTTATMAPVINRDDQCPICAAKSTGVIDLQPLSE
                     GFTFINKNLYPILHPVENLQESDGGMPLYPDPYHHGRSSYGMHLLQWTSSIHDRDWHN
                     MPLADCLICFDRLAVLEHKLLTDSAGFMPASDRVLQKLPRRSGEADIACGPKTFGHVS
                     IIKNFGRAAGASMSHGHQQIGFSNIMPQRYFNNFSFMQRNGQTFSEYMLRENPQRLMV
                     HDFGQAVLIVPYFMKRPYDMLVLVKDHQKQYIHQLTPDERRDLVRAMVAAIQAIMTIM
                     PQLGKAPAYNININNGPGAGVYLELLASTQLTGGFEHIGLWVCQANPNEVAAQLRQVV
                     KV"
BASE COUNT          764 a          951 c         1018 g          788 t
ORIGIN      
        1 ttggcggctt tttcttcagc cagtttctga atggcttcgg ccagttcctc ttcagcggtt
       61 ttttccagtt tcttttctgt cgggtcgtcg atcaacccga gcaaaaatgc cacccgcttg
      121 atcagttcgg acagaccttg cagccacagc aggccaaagc ccagcggcat catggcgtac
      181 accggccagc gaatcagccc gcccgcattg gacgacattt caccgctgtg aaagcctttc
      241 agaaagaacg gtgtgctacc agatgatggc ggagcaaaac ggcagcagga aaaagacgaa
      301 gccaaacaca tcgatccagg tctgggtgcg cttgtataga cggctgtaaa tgacatcgat
      361 cttgacgtgt tcgccattga gcagggtaaa ggccgcgcac agcatgaagg tcgcggcaaa
      421 caggtaccac tgcacctcca ggtaggcgtt tgagctgaag ttgaaggcct tgcgcacaac
      481 ggcattgatg gcgctgatga cggtcgaagc caggatcagc cagatcacat atttgccaat
      541 aaagcggttc agcgcgtcaa tggcattgga aaatttcaaa agagcctgca tgttgtctcc
      601 gttttgtaac tgtttgcccg atcgacaggg ctgttgtaat gggtggcggc acgcagttta
      661 tcgattgggt tgaaagtcag tgcgtgaata attttagggg caagcctgaa atttttttac
      721 agacttaccc ttggacattt ggccgcaatg cgctcggggc cgggctttac gcctggctgc
      781 cgtttttgca gactcttcgt ggcaataccc ggcgctgttg agcaggcacc cattgttggc
      841 tgggaccgca ggtcggattg cagcacctga tgagtcggct gacagagcat tccggcgtga
      901 tgagggtgat gcccaggtcg gttacgtgaa ggcgggcgcg gtcggcatcc gggttgacgc
      961 cgacttgaaa gccgtctgga atgaagcagc cgttgtccac caccacgcgc ttgaggatga
     1021 cgttgcgacc caccaccaca tcaggcagca acaccgagtc ctcgatcagg ctgtacgcgg
     1081 caacccgcac ttttgtaaac agcactgagc cgtacacctt ggcaccgctg acaatgcagc
     1141 cattctgtgt agtgacgctg attcgcgccg tcggatgaaa actcgaaatg acagcttgtg
     1201 ccgcaggccg cttcaagcag ctaccgattc acttgtgcct ggtttgaatc cccgacgatg
     1261 ctggcaaagg tcacacctgc aatggcatga acatcggaca cgccgacaaa ctgcagtccg
     1321 gcctggttgg cccacaggat ggttccatcc agtccagttt cgagcagatg aaacggcacc
     1381 agctcgacca gcacgcggta gcgatcttct gccgcaagat gactcacatc ggcaatgctg
     1441 gcggccggga ctgtattggc aggcagagaa tcgtcaggca tgaaagttgg actcgcttgc
     1501 tgggttggac cgtgtgtaaa aaaggcggca agaggcaatc acactgtctc tcaggcattg
     1561 atgcgtcagc gtgctcgggt tgtcaatgcg acccggccaa gcgcgaatgc acgcgccatg
     1621 gcccgaatat ctgatgcccg cagtgctgat cggcttacgc ccaattcgaa agtcaatttt
     1681 ggtcacgtcg ttggggctct ttgcgtttcg gcgaatggcc ggtgttcgcc ccggccgatc
     1741 atggtcaacg caacttcgag tgtctggaat cagtgtttca agctggattc cacagttgga
     1801 tgggtagcgc cgtcacccaa tgggtgaacc ccatccaaga tgaaaaaatc aaaattcctg
     1861 agaacttaca gatgaaaaca agcggtcaac tgaatctagg agcctgtcgg acttatggaa
     1921 catcggctgc aaatagaccg cagcggccca tttcgcaccg gctttttgac ccatagcggg
     1981 gctatgggcc tgcaaatccg gcgcaaactg tgctcgctgg ggtcgatttt cgctatcgat
     2041 gccataagtc cgacaggcac ctaggctcaa accttgacga cttggcgaag ctgcgccgca
     2101 acctcatttg gattggcttg acagacccac aggccgatgt gttcaaaccc gcctgtcaat
     2161 tgtgtactgg ccagcaactc cagatacacg ccggcaccgg gcccgttgtt gatgttgatg
     2221 ttgtacgccg gtgccttgcc cagttgcggc atgatggtca tgatcgcctg gatggccgcc
     2281 accatggccc ggaccagatc ccggcgctca tccggggtca gttggtggat gtactgcttc
     2341 tggtggtcct tgaccagcac cagcatgtcg taaggtcgct tcatgaaata ggggacgatc
     2401 aacaccgctt gaccaaagtc gtgcaccatc agtcgctgcg ggttctctct gagcatgtac
     2461 tctgaaaaag tctgcccgtt gcgctgcatg aagctgaagt tgttgaaata gcgctggggc
     2521 atgatgttac tgaagccgat ttgctggtgt ccgtgcgaca tcgatgcacc ggccgcgcgc
     2581 ccgaagttct tgatgatgga gacgtgcccg aaggtttttg gcccacaggc gatgtccgcc
     2641 tcacctgatc ggcgtggcag tttctgcagc actcggtctg aagcaggcat gaaccctgcc
     2701 gagtccgtca gcaacttatg ttccagaacc gccagtcggt caaaacagat caggcagtct
     2761 gccagtggca tgttgtgcca gtcccggtca tgtatcgagg aagtccattg cagcagatgc
     2821 atgccatacg aactgcgacc gtggtgatag gggtccggat acagcggcat gcccccgtcg
     2881 ctttcctgca gattctcgac cgggtggagg atcggataaa gattcttgtt gatgaaggtg
     2941 aagccttcgc tcagcggttg cagatcaatc accccggtgc tcttggcagc gcagatcggg
     3001 cattggtcgt cacggttgat gacgggtgcc atggtggcgg tcgtgtgcag ccgggtggct
     3061 cgcgacgagt tgtagacgat cacgtccccg gttcggggat caatctgaca aagggaatct
     3121 ggcaagaagg ccgccatctg cgcgtcgtca agaatgtctt ccaggatctc gcggatcgaa
     3181 aggtcattga cattgcgggc gctgatcagc gcttcaatgg cttccaattt aagttgctgc
     3241 ggctcgccca tggcgtggat attaccgtac tggccctggc ttgcgtggag aggtgccgag
     3301 ctgttgcgcc aatctatccg ccaaaaagcc gagtcgggcg agtccgcatc gtcctggact
     3361 caacccagac gacgaacctg gccgtatggc gcattggcag gcccaatgac cggtcttgag
     3421 aattcagttc acccaattgg cagtttggtg tcgaaatccc agtcaataca ttttttatag
     3481 ctggcagcgc aatcaccgct tgggatacag gccatttttt t
//
LOCUS       DAOSLL010000522         3517 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_23336_length_3517_cov_3.391681, whole genome shotgun sequence.
ACCESSION   DAOSLL010000522 DAOSLL010000000
VERSION     DAOSLL010000522.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23336_length_3517_cov_3.391681"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="PLB25_18160"
     CDS             <1..980
                     /locus_tag="PLB25_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019140774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HOE43530.1"
                     /translation="KHFVETVKSDDFDLIDTLAALYDEPYADSSAIPTYRVCQLARKH
                     VTVALSGDCGDEAMGGYRRYRMHLLEERMRSAFPLGLRRPLFGTLGKLYPKADWAPRM
                     FRAKTTFEGLARNSVEAYFNTVSILRRPMRDALFSAGFKTALAGYNAQEVFNHHAKNA
                     GTDDPLALIQYLDLKTYLVGDINTKVDRASMAHSLEVRAPLMDYELIDWLATLPSSHK
                     IQGQEGKHIFKKAMQPYLPNDVLYRPKMGFSVPLARWFRGPLKQRVRDAVLGDRLACT
                     GWFNQDYLQQLVNGHQSGAYDYSAPLWTLLMFEAFLRQVVDAPVAEASA"
     gene            complement(1039..2283)
                     /locus_tag="PLB25_18165"
     CDS             complement(1039..2283)
                     /locus_tag="PLB25_18165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase, exosortase A
                     system-associated"
                     /protein_id="HOE43531.1"
                     /translation="MSTPERQLPVHAPMNQFAVQDGELVVGGKRLSTLVAQVGQTPFY
                     AYDRALLKARVAELRSVLPPEIQLHYAMKANPMPAVVALMAGLVDGIDVASAGELKVA
                     LDAGANPHEVSFAGPGKRDAELHQAVAAGVLVNLESFRELAVLAAASQALGLPARVAV
                     RVNPDFELKGSGMKMSGGPKQFGVDAEQVPALLQGIDDCGLAFEGFHLFAGSQNLRAE
                     AICEAQQKSYELALRLAQTARQPVRFLNLGGGFGIPYFPGEKRLDLAPIGDNLAQLVQ
                     RAQGELPQAQLVIELGRYFVGDAGVYVARVIDRKVSRGLVYLVTDGGLHQHLAASGNF
                     GQVVRKNYPVCIGNRMGTGETETASVVGPLCTPLDLLADRMTLPVAQIADLVVVFQSG
                     AYGISASPQRFLGHPACVEVLV"
     gene            complement(2313..>3517)
                     /locus_tag="PLB25_18170"
     CDS             complement(2313..>3517)
                     /locus_tag="PLB25_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA ligase (AMP-forming), exosortase A
                     system-associated"
                     /protein_id="HOE43532.1"
                     /translation="GAFADGAPLATPVITWNDLLASPARVGHRVIDTDVVGILYTSGS
                     TGKPKGVVLSHRNMVAGAKSVASYLENNAQDTLLAALPLSFDAGFSQLTTAFHVGARV
                     VLLNYLMPRDVLKAMAREKVTGLTAVPPLYIQLSQLAWSADLNEHLRYFANTGGRMPR
                     ETLDQLRKHVPAAKPFLMYGLTEAFRSTYLPPDEVDRRPDSIGKAIPNAEILVLREDG
                     TPCEPDEPGELVHRGALVGLGYWNDAEKTAERYKLLACDAPGRQAGLQLPEYAVFSGD
                     TVRQDADGFLYFIGRRDEMMKTSGYRVSPTEVEEILYATQMVGECVAFGVAHEMLGHA
                     IHVIATIADTAGSRGLNDLMTECRTRMPAYMVPAGIELVTGPLPRNPNGKIDRKLLAT
                     EWLARHTV"
BASE COUNT          709 a         1135 c          989 g          684 t
ORIGIN      
        1 cgaagcactt cgtcgagacg gtcaagtccg acgacttcga cctcatcgac accctggccg
       61 cgctctatga cgagccctac gccgacagct ccgccatccc cacctaccgg gtgtgccagc
      121 tcgcgcgcaa gcacgtcacc gtggccctgt cgggtgactg cggcgacgaa gccatgggtg
      181 gctaccgccg ctaccggatg cacctgctgg aagagcgcat gcgctcggcc ttccctttgg
      241 gcttgcgccg gcccttgttt ggcacactgg gcaagctcta ccccaaagcc gattgggcac
      301 cgcgcatgtt ccgtgccaaa accactttcg aggggctcgc gcgcaactcg gttgaggcct
      361 atttcaacac cgtgtcgatt ctgcgccgac ccatgcgtga tgccttgttc agcgctggct
      421 tcaagaccgc gctggcgggc tacaacgcac aagaggtgtt caaccatcat gccaaaaacg
      481 ctggcactga cgacccgctg gcgctgattc aatacctcga cctcaaaacc tacctggtgg
      541 gcgacatcaa taccaaggtt gaccgtgcca gcatggcgca ctcgctcgaa gtgcgcgcgc
      601 cgctcatgga ctatgagctg attgactggc tggcaacgct accgtccagc cacaagattc
      661 agggtcagga aggcaaacac atcttcaaaa aggcgatgca gccctacctg cccaacgacg
      721 tgctctaccg ccccaaaatg ggcttttcgg taccgttggc gcggtggttt cgcggcccat
      781 tgaagcagcg cgtgcgcgat gcggtgctgg gtgatcgcct ggcctgtacc ggctggttca
      841 atcaagacta tttacagcaa ctggtgaatg gccatcagtc aggggcctat gactacagcg
      901 caccgctttg gacgctgttg atgtttgaag cctttttgcg ccaggtagtc gatgcgccgg
      961 tggccgaggc aagcgcgtga gccagcgcag cgtttttgac tgctttggcc agatgcccgg
     1021 tcgcttgctg aaggttctct aaaccaaaac ttccacacaa gctgggtggc ccaaaaaccg
     1081 ctgcgggctg gcgctgatgc cataggcacc cgactgaaac accaccacca ggtctgcgat
     1141 ctgcgccact ggcagcgtca tgcgatctgc cagcaggtcc agcggtgtgc acaatgggcc
     1201 taccacgctg gcggtttcgg tctcaccggt acccatgcgg ttgccaatgc acaccggata
     1261 atttttgcgc accacctgac caaagttgcc cgatgcagcc aaatgctgat gcagcccgcc
     1321 atcagtcacc aggtacacca gaccgcgaga caccttgcgg tcaatcacgc gcgccacata
     1381 cacccctgcg tcgccgacaa aatagcgccc cagttcaatc accagttggg cctgcggcaa
     1441 ttcaccctgc gcacgttgca ccaactgcgc caggttgtca ccaataggtg ccaggtcgag
     1501 tcgtttttca cctggaaagt acggtatgcc aaagccaccg ccgaggttca aaaagcgcac
     1561 cggttgcctg gccgtctgcg ccagcctcaa cgccagctcg taagactttt gctgggcttc
     1621 gcaaatcgcc tcggcgcgca aattctgcga tccggcaaac aggtgaaagc cctcaaatgc
     1681 caagccgcag tcgtcaatgc cctgcaacag tgctggtact tgttcggcat ccacgccgaa
     1741 ctgcttgggc ccaccactca ttttcatgcc cgaacccttg agctcaaagt cagggttcac
     1801 acgcaccgcc acgcgcgcgg gcaggcccag cgcctggctg gctgctgcca gcacagcaag
     1861 ctctcgaaac gactcaaggt tgaccagcac cccagcggct acagcctggt gcagctctgc
     1921 atcgcgtttg cctggtccgg caaaactcac ctcgtgcggg tttgcccctg cgtccaatgc
     1981 caccttgagt tcacctgccg atgccacgtc gatgccgtcc accagcccag ccatcagcgc
     2041 gaccacggcc ggcataggat tggccttcat cgcgtaatgc aactgaatct caggtggcag
     2101 cacgctgcgc agttcggcca cccgcgcttt gagcaatgcg cggtcgtagg cataaaacgg
     2161 tgtctgcccc acctgagcga ccagggtgga caagcgtttg ccgccaacca ccagttcgcc
     2221 atcctgcacg gcaaactggt tcatcggggc gtggacgggt agctggcgct cgggggtgga
     2281 catgatgctg ttgatcgcgt tgtggcaagg ggttagacag tgtgccgcgc cagccactcg
     2341 gtggccagca atttgcggtc gattttgccg ttggggttgc gtggcagcgg cccggtgacc
     2401 agttcgatgc cggccggaac catgtaggcc ggcatcctgg tgcggcactc agtcataaga
     2461 tcatttaggc ctctagaccc cgctgtatct gcgatagtag ctatcacatg tatagcatgc
     2521 ccaagcattt catgcgccac accaaaggct acacattcgc ctaccatctg ggttgcatac
     2581 aaaatttctt ccacctcagt cgggctcacc cggtagccag aggtcttcat catctcatcg
     2641 cgtcggccaa taaagtacaa aaagccgtct gcatcctggc gcaccgtatc gcctgaaaac
     2701 accgcgtatt caggaagctg caagcccgct tgccgccctg gagcgtcgca ggccagcagc
     2761 ttgtagcgct cggcggtctt ttcggcatcg ttccagtagc ccaggcccac cagcgcaccg
     2821 cgatgcacca gctcaccggg ttcgtctggc tcacagggcg tgccatcttc gcgcagcacc
     2881 aaaatctcgg cattcgggat cgccttgccg atcgaatcgg ggcgacggtc aacttcgtcg
     2941 ggcggcagat atgtggaccg aaaggcctct gtcagtccgt acatcaagaa aggtttggca
     3001 gccggcacat gcttgcgcaa ctggtccagc gtctcgcgcg gcatgcgccc gccggtgttg
     3061 gcgaaataac gcaagtgttc attcaggtca gccgaccatg ccagttgtga cagttgaatg
     3121 tagagcggtg gcaccgctgt caggccggtg accttctcac gcgccatggc tttaagcacg
     3181 tcacgcggca tcaaatagtt cagcaaaacc acccgtgcgc cgacgtggaa ggcggtggtc
     3241 aactggctga atccggcatc aaaagacagc ggcagagcgg ccagcagcgt gtcctgagcg
     3301 ttattttcca gatacgatgc cacgctcttg gcgccagcga ccatgttccg gtgagacagc
     3361 accacgcctt tgggttttcc ggtgctgccc gatgtgtaca aaatgcccac cacgtcggtg
     3421 tcaatcaccc tgtggccgac gcgcgccggg ctggccagca gatcattcca ggtgatcacc
     3481 ggcgttgcca ggggcgcgcc atctgcgaat gcaccag
//
LOCUS       DAOSLL010000523         3515 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_23354_length_3515_cov_3.608960, whole genome shotgun sequence.
ACCESSION   DAOSLL010000523 DAOSLL010000000
VERSION     DAOSLL010000523.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3515)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23354_length_3515_cov_3.608960"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="PLB25_18175"
     CDS             complement(<1..563)
                     /locus_tag="PLB25_18175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE43533.1"
                     /translation="MQVDTPHTSNATGASASTAAAVRGLAILCVDDEPSILSSLRRLF
                     RPKGYQIYIAEGGKAALALLEQNAVDLVISDMRMPEMDGVQFLEQVRLRWPDTVRLLL
                     TGYADIASTIDAVNRGEIYRYISKPWDDADILLIVHDALEQRALELENKRLKSVAQAQ
                     NAELKNAHAYLESLVAQRITELTVANDA"
     gene            complement(572..1414)
                     /locus_tag="PLB25_18180"
     CDS             complement(572..1414)
                     /locus_tag="PLB25_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOE43534.1"
                     /translation="MSTELTLRLAAAVESMPAFPKTVQSILALTGNLDCSPKDLVHVI
                     GKDPVITIKVLRVVNSAYYSLSNQITSIDHAVVFLGFNTIKNLALSIAAINMLPANHL
                     AGFDAQQYLLHSLVTAGIAKKFALHVQQADPNDCFVAGLLHDFGKVVVAQCMPAQYQR
                     AVEISLWKETSLHAALREVIGVDHFAIGAMLVQRWRFAPQLIETIGCLNTATLKDTDM
                     IACVFAANQICKHIGCDFGGSPALDALPAAVSQRLSGTLDELRLSLGDLTPMLEEARL
                     FAKL"
     gene            complement(1411..>3515)
                     /locus_tag="PLB25_18185"
     CDS             complement(1411..>3515)
                     /locus_tag="PLB25_18185"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HOE43535.1"
                     /translation="PTSVALPTSDIARMGSVFTDHKKSGLATSVQTIFNTYTQAKRLV
                     VMTTVQPAALAMDHSLTVTVSRDIGHIFSQWRKQAYLATSIFLGLSLFSILSLRLYQR
                     RLHDLGRIAQDETTAREQAEQKMRATAELMQSFLDHLPGMAYVKDSDCRILMANRGFQ
                     MLDLDPATMIGKTNLELLPGSLGEAITTDDQRVLAHGKIELIQEEIGGRSFETSKFVI
                     DDAHGQRLLGGITLDVTARQQHNRLTQALLEINEFGMRLSEKDFLTHGLELAERLTTS
                     GIGFLHFVSEDQETLELVTWTSKTLQGGTAVHDAHYPISQAGIWADCFRQRQPVVFND
                     YDGHTQKQGLPVGHAPLQRLISVPVMDDGAVRMVIGVGNKNHDYDAADIETVQLIGND
                     LWGIARRNRAERKMQQQLLELVALNSKLLETQGQLLQSEKMAAVGQLAAGIAHEINNP
                     VGFVFSNLTTLADYVEDLLAIDSVYSEMEDRLQSLAPELLARVHALKTACDHPFVVID
                     VRHLLRESGEGLKRVKTIVQDLKDFSRAGDTAWEWADLQKGLESTLNIVRNEIKYKAS
                     IVRELAPLPPVRCIPAQINQVFMNLLVNAAQAIQEHGTIVLRSGVDGDTVWISVQDDG
                     CGIAPEKVSRIFDPFYTSKPVGKGTGLGLTLAWGIMQRHQGSIDVQSTPGKGSCFTVR
                     LPINGPTNDPNSEGNAPP"
BASE COUNT          744 a          993 c          984 g          794 t
ORIGIN      
        1 gcgtcatttg cgacggtgag ttcggtgata cgttgtgcaa ccaaggactc caaataggca
       61 tgggcgtttt tgagctccgc attttgggct tgtgcaacag acttaagccg tttgttttcc
      121 agctccaggg cgcgctgctc cagtgcatcg tgcacgatca gcaaaatatc ggcgtcgtcc
      181 cagggcttgg agatatagcg ataaatctcg ccccggttca ccgcatcaat ggtggaagca
      241 atatcggcat aaccggtcaa cagcagtcgc accgtatcgg gccagcgcag gcgcacttgc
      301 tccaggaatt gaacaccgtc catttccggc atccgcatgt ccgaaatgac caggtcaacc
      361 gcattctgct ccagcaacgc cagcgcggct tttcctcctt cggcaatata aatctggtag
      421 cccttggggc gaaacaggcg tcgcagcgaa gacaggatgc tgggctcgtc gtccacacac
      481 aaaatcgcca acccgcgcac cgccgccgcc gtgctggcag atgcgccggt tgcgttggat
      541 gtgtgggggg tgtctacttg catggggtgg cttaaagctt ggcgaagagc cgggcttctt
      601 ccagcatggg ggtgaggtcg cccaaagaca ggcgcagttc atccagcgtg ccgctcaggc
      661 gctggctcac tgcagccggc aaggcatcca gcgccgggct tccgccaaag tcacaaccga
      721 tgtgcttgca tatctggttg gcggcgaata cgcaggcaat catgtcggtg tctttgagag
      781 tggccgtgtt caggcaacca atggtctcaa tcaactgggg cgcaaaacgc cagcgctgaa
      841 ccaacatggc accaatggca aagtggtcca caccaatcac ctcgcgcaaa gcggcgtgca
      901 gggaagtttc tttccacagg ctgatctcga cagcgcgttg gtactgcgca ggcatgcact
      961 gcgccaccac caccttgcca aagtcatgca acagaccggc aacaaaacag tcgtttgggt
     1021 cagcctgttg cacgtgcagt gcaaatttct tggcaatgcc agcggtaacc agagagtgca
     1081 gcaggtactg ttgggcatca aaacccgcca ggtggttggc tggcaacatg ttgatggcgg
     1141 caatactgag tgccaggttt ttgatggtgt tgaaccccaa aaaaactacc gcatggtcaa
     1201 tcgaggtgat ctggttggac aagctgtagt aggccgagtt gaccacccgc aacaccttga
     1261 tggtgatgac cgggtctttg ccaatgacgt gcaccaggtc cttgggcgaa caatccaaat
     1321 tgcctgtcag agccaggatg ctttgaacag ttttgggaaa ggctggcatg ctctccaccg
     1381 cagccgccag cctgagggtc agctcggtgc tcacggtggg gcattgcctt cactatttgg
     1441 atcattggtt ggcccattga tgggcaggcg aaccgtgaag caactgccct tgccaggggt
     1501 ggactgcaca tcaatcgagc cttggtggcg ctgcatgatg ccccaggcca gcgtcaggcc
     1561 cagcccagtg cccttgccga cgggtttgct ggtgtaaaac ggatcaaaga tgcgcgagac
     1621 cttttctggc gcaatgccgc agccgtcgtc ttgcacggaa atccagacgg tgtcgccatc
     1681 gacaccgctg cgcaacacaa tggtgccgtg ctcttgaatg gcctgcgccg cattcaccag
     1741 caagttcatg aaaacctggt tgatctgcgc cgggatgcag cgcacaggtg gcaagggagc
     1801 cagttcgcga acaatgctag ccttgtattt gatctcgttg cgaacaatat tgagtgtgct
     1861 ttccaggccc ttctgcaaat cagcccattc ccaggccgtg tcacctgcgc gtgaaaaatc
     1921 tttcaggtct tgcacaatgg ttttgacacg cttcaggcct tcgccggatt cacgcaacag
     1981 atggcgcaca tcgatcacta caaaggggtg gtcgcaggcc gtcttcagtg cgtgtactcg
     2041 cgccagcagt tccggggcga ggctttgcag ccggtcttcc atctcgctgt agacgctgtc
     2101 aatggccagc aggtcctcca catagtcagc caaggtggtc aaattggaaa agacaaaccc
     2161 aaccgggttg ttgatttcat gcgcaattcc ggctgccaat tgcccaacag ccgccatttt
     2221 ttcggattgc aggagttggc cctgggtttc gagcagcttg ctgttgaggg caaccagttc
     2281 aagcaattgc tgttgcattt ttcgctcggc gcgatttcgc ctggcgatgc cccacagatc
     2341 gttgccaata agctgtacgg tctcaatatc cgccgcgtca tagtcatggt ttttgttgcc
     2401 aacgccgatc accattcgaa ctgcgccatc gtccatcaca ggcaccgaaa tcaaccgctg
     2461 taatggtgcg tgcccaaccg ggagcccctg cttttgggtg tggccgtcat agtcgttaaa
     2521 cactacgggt tgcctctggc ggaaacagtc tgcccagatg ccagcctggc tgatggggta
     2581 gtgtgcatca tgcaccgcgg tgccgccttg gagcgtcttg ctggtccagg tcaccaactc
     2641 cagcgtttcc tggtcttcac tgacgaaatg cagaaaaccg atgccgctgg tggtcaggcg
     2701 ctctgccagt tccaggccat gggtcagaaa gtccttttcg ctcaggcgca tgccaaattc
     2761 attgatttcc agcagcgcct gggtcagccg gttgtgttgt tgccgggctg tcacgtccaa
     2821 cgtgatgccg cccagcaggc gttggccgtg cgcgtcgtca atcacaaatt ttgacgtctc
     2881 aaaactgcgg cccccaattt cctcctgaat caattcaatc ttgccgtggg ccagcacgcg
     2941 ctggtcgtcg gtcgtaattg cttcgcccaa tgatcctggc aacaattcca gattggtctt
     3001 tccgatcatc gtggcggggt ccaggtcaag catctgaaac ccccggttgg ccattagtat
     3061 gcggcagtca ctgtctttga cataggccat gcctggtaaa tggtcaagaa agctttgcat
     3121 tagttcagcg gtagcgcgca ttttttgctc tgcctgctca cgagctgttg tctcgtcttg
     3181 cgcgattcgc cccagatcgt gcagacgccg ttgataaaga cgcaaactca ggatgctgaa
     3241 caaggacaaa cccagaaaga tgcttgttgc caaataagcc tgctttcgcc attggctgaa
     3301 gatatgtcca atgtcacggc taaccgttac agtcagcgag tgatccatcg ccaaagccgc
     3361 aggttgcacc gtggtcataa ccaccaagcg tttagcttgc gtataagtgt taaagatggt
     3421 ctgcacgctg gttgccaacc cgctcttttt gtggtccgta aaaaccgaac ccatccgggc
     3481 gatatcgctg gtgggcagcg ccacagatgt cgggg
//
LOCUS       DAOSLL010000524         3501 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_23437_length_3501_cov_4.876959, whole genome shotgun sequence.
ACCESSION   DAOSLL010000524 DAOSLL010000000
VERSION     DAOSLL010000524.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23437_length_3501_cov_4.876959"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PLB25_18190"
     CDS             <1..297
                     /locus_tag="PLB25_18190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43536.1"
                     /translation="DLKLTLAQCLPNAAPASSAVRPTTAPLQAVDWPRVEALLAETLP
                     LLEQQKFDAIEHFRALQAAAGDSSLADGLKAIAPLVTNLRFEEALALLRQLPHS"
     gene            719..3229
                     /locus_tag="PLB25_18195"
     CDS             719..3229
                     /locus_tag="PLB25_18195"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE43537.1"
                     /translation="MEKSKPKILAIDDTPVNLLTLGAALKDDFDLQVATSGLSGISLA
                     MQDPPDLILLDIMMPDLDGFETCQQLKADPLLRDIPVIFITALHELDSEIKGLELGAA
                     DYITKPIQVETARQRIRNLLEREQLRRQVTAQRDQLADEIKEHNKTQANLRKLSVAIE
                     QSPASVVITDLNAVIQYVNPRFTEVTGYSAAEAIGQNPRILKSDQTSSEVFSELWAYL
                     TQGKVWKGELINKRKNGEVYWEESHISPVKSAAGEVTHYVAVKTDITERKLAQTKLQL
                     AAGVFASAREGIAITNLKGDMIDVNQAFTRITGYSREEALGKNSRMLNSGRQNKEFYN
                     AMWEQLTINGHWFGEIWNRRKNGEIYAEQLTISSVLDAHGKPQNYVALFSDITAQKAQ
                     QRELDHIAHFDVLTGLPNRVLLADRLRQGMAQSVRRGQQLAVIFLDLDGFKLINDNHG
                     HEAGDLLLMTVAKRMKQALREGDTLARIGGDEFVAVLNDMDETASSMPLLSRLLEAAA
                     MPLQLINLTLKISASMGVTFYPQTLDIDVEQLLRQADQAMYQAKLAGKNRFHLFDTEQ
                     DTGLSSHNETIKGIRQALGSNELVLHYQPKVNMRTGQVVGAEALLRWQHPEQGLQMPG
                     SFLPSLDDHPLAVDVGEWVIESALAQMEAWQAQGFDLPVSVNVCTRQLQQIDFVTRLR
                     DILARHPQVKPSHLELEVLEASALANINGVTQIVEQCQEMGVAFSLDDFGTGYSSLTY
                     LKRLPVAILKIDQSFVRDMLVDPEDLAVLEGVIGLARVFHRVVIAEGVETIAHGLLLL
                     KLGCDLAQGFGIARPMAAQELPRWASQWQPDPAWMAQG"
BASE COUNT          784 a          996 c         1009 g          712 t
ORIGIN      
        1 gacctgaagc tgacgctggc ccagtgcttg cccaacgcag caccggccag tagcgctgtg
       61 cgaccaacga cagctcctct gcaggccgtc gactggccgc gcgtcgaggc gctgctggca
      121 gaaacgctgc ctctgctgga acaacaaaaa ttcgatgcca ttgaacattt ccgtgcgttg
      181 caggcggcgg ctggcgattc ttcactggcc gatggcctga aggcgattgc gcccctggta
      241 accaacttgc gttttgaaga agcgctggcg ctgttgcgcc aactgccgca ttcttgacag
      301 tcctgttgat ctgatcctga cgggtctgtc acgtcacctg tgtggatgag gtgattcagt
      361 cgcaggcaaa ggctttcctg cagggcaatt gggaggcttt ttttgctgcc cgtgctggcc
      421 attctgccga gccggttccc gtttgtgatc aaggcgattg accaaacctc aaacctgcaa
      481 tctgccaatg gcaccattac gccaaaggca gggccatgca tgaatgtatg gcacgatgtc
      541 gggcatcggt ccggctctga caggggccga aaatgaatga acaaaaaaaa tggcctgaaa
      601 gacaaaagca tgccgtttac actggcatat ttgacccggt cagcctgaat caagattgag
      661 gccaaactgg cttcgtttca ggctcatacc tgaactggaa aatacaatca gaggtgacat
      721 ggaaaaaagc aaacccaaaa ttctggccat tgatgacacc ccggtcaacc tgttgaccct
      781 gggtgcggcc ctgaaggacg atttcgatct gcaggtggcc acatcaggcc tgtcaggaat
      841 cagtctggcg atgcaagacc cgcctgatct gatcctgctg gacatcatga tgcccgactt
      901 ggatggtttc gagacctgcc agcaactcaa ggccgatccg ttgctgcgcg acattccggt
      961 gatttttatc actgccctgc atgagcttga ctcggagatc aagggcttgg aactcggtgc
     1021 tgcagactac atcaccaagc cgattcaggt cgagacggcg cgccagcgca ttcgcaacct
     1081 gcttgagcgc gagcaattgc gtcgccaggt gacggctcag cgcgatcagc tcgccgatga
     1141 aatcaaagag cacaacaaga cccaggcaaa cttgcggaaa ctgagtgtgg caattgaaca
     1201 aagcccggca tcggtggtaa ttaccgacct gaacgccgtg attcagtacg tcaacccgcg
     1261 ctttaccgaa gtcactggtt acagcgctgc tgaggcgatt ggacaaaatc cgcgcatcct
     1321 gaagtctgac cagaccagca gtgaggtgtt ctccgaattg tgggcgtacc ttacgcaagg
     1381 caaagtctgg aagggcgagc tgatcaacaa gcgtaagaac ggcgaggtgt attgggaaga
     1441 atcgcatatc tcgccggtca aaagcgcggc gggtgaggtc actcactatg tggcggtcaa
     1501 gacggacatc accgagcgca aactggcgca gaccaaactg caacttgccg ccggcgtgtt
     1561 tgccagtgcc cgcgagggaa ttgccatcac caatctcaag ggtgacatga tcgatgtcaa
     1621 ccaggccttc acccgcatca ccggttacag ccgggaagag gccctgggca agaattcgcg
     1681 catgctgaac tcaggccgcc aaaacaaaga gttctacaac gccatgtggg agcaactgac
     1741 catcaatggt cactggtttg gcgagatctg gaatcgtcgc aagaacggtg agatttatgc
     1801 cgaacagctc accatttcct cggtgcttga tgcccacggc aagccacaaa attacgtggc
     1861 gctgttttcc gatatcactg cccaaaaggc acagcagcgg gaacttgacc acattgccca
     1921 cttcgacgta ttgaccggcc tgccgaaccg ggttttgctg gccgaccggc tgcgccaggg
     1981 catggcgcaa tcggtgcgcc gtggccagca actggcggtc atttttctag accttgacgg
     2041 cttcaagctg atcaacgaca accacggtca tgaagccggc gacctgttgc tgatgacggt
     2101 ggccaagcgc atgaagcagg ccttgcgcga aggcgacacg ctcgcacgca tcggcggcga
     2161 cgaatttgtg gcggtgctca acgacatgga cgaaacggct tctagcatgc cgctgctcag
     2221 ccgcctgctc gaggccgccg ccatgccgct tcagttgatc aacctgacac tcaagatctc
     2281 agccagcatg ggcgtgacgt tttacccgca aaccctcgac attgatgtcg agcaattgtt
     2341 gcgtcaggca gatcaggcca tgtaccaggc caaactggcc ggcaaaaacc gctttcattt
     2401 gtttgatacc gagcaagaca ctggtctgag cagccacaac gaaaccatca agggcatccg
     2461 ccaggcattg ggctccaatg aactggtgct gcattaccag cccaaggtca acatgcgcac
     2521 cgggcaggtg gtcggggccg aggcgctgct gcgctggcag catcccgagc aaggcctgca
     2581 aatgccgggc tcgtttctgc cgtcgctgga cgaccatcca ctggccgtcg atgtgggtga
     2641 atgggtcatc gaatcagcgc tggcgcaaat ggaagcctgg caagcccagg gttttgactt
     2701 gccggtcagc gtcaacgtct gtacccgcca gttgcagcag atcgactttg tcacccgctt
     2761 gcgtgacatt ctggccagac acccgcaggt caagccaagc catctggaac ttgaggtgct
     2821 cgaagccagc gccctggcca atatcaacgg tgtgacacaa atcgtcgaac aatgccagga
     2881 aatgggggtg gctttttcgc ttgacgactt tggcaccgga tactcgtcgt tgacttatct
     2941 gaaacgcctg ccggttgcga tcttgaaaat cgaccaaagc tttgtgcgcg acatgctggt
     3001 ggacccggag gatcttgcgg tgctggaagg tgttatcggg ttggcgcggg tgtttcatcg
     3061 ggtggtaata gccgaaggcg tggaaaccat tgcgcacggc ttgctgctgc tcaaacttgg
     3121 ctgcgacctg gcgcagggtt ttggcattgc ccgaccgatg gccgcgcaag aactgccacg
     3181 ctgggcatcg cagtggcaac ctgatccggc ctggatggcc cagggctaac aatcggcccg
     3241 acgaggacca gtcaccggca accctatgcg cctgtcaaga atccgggggt gagtttgaac
     3301 ctggatttgt ctgttgaacg cacccgaacg agccgctggc aacgcgcctg tggtcattgt
     3361 taacccctcg tcgcatcgcg catctcccct gtcgcggctt gctgcgacat gcagcttgca
     3421 gccttgccag tggccttgcc tgtttaagga tgtggtcagg aataacttcc cctatttgca
     3481 ccggcggatt tgggcgcaca g
//
LOCUS       DAOSLL010000525         3494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_23493_length_3494_cov_4.981099, whole genome shotgun sequence.
ACCESSION   DAOSLL010000525 DAOSLL010000000
VERSION     DAOSLL010000525.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23493_length_3494_cov_4.981099"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="PLB25_18200"
     CDS             <1..1155
                     /locus_tag="PLB25_18200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HOE43538.1"
                     /translation="TGHTDARGRLFFQTQLLNYDLPTSLPQGKADNQILGVVEALRKQ
                     HAPREVVLVSKDINMRVKARALGLSTDDYQSDKTLEDGDLLYSGAYALPTDFWSTHGS
                     TVESWQQGVHTFYRVSGPVVPQLLVNQFVYFEAPGEPSLYAKVTEIRGKVAVFRTLRD
                     FTHLKNAVWGITTRNREQNFAMNLLLDPEVDFVTLTGQAGTGKTLLALASGLTQVLDE
                     RRYTEIIVTRATVSVGEDIGFLPGTEEEKMGPWMGALDDNLEVLGKTDTAAGEWGRAA
                     TNELIRSRIKIKSMNFMRGRTFLNKYVIIDEAQNLTPKQMKTLVTRAGPGTKIICMGN
                     IAQIDTPYLTEGSSGLTYAVDKFKGWQHSGHITLARGERSRLADFASEVL"
     gene            complement(1272..2681)
                     /gene="dnaB"
                     /locus_tag="PLB25_18205"
     CDS             complement(1272..2681)
                     /gene="dnaB"
                     /locus_tag="PLB25_18205"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HOE43539.1"
                     /translation="MSAVFSVINDDPGLDRQVAQLRIPPHSIEAESSVLGGLLLDNAA
                     WDRVSDVLTEQDFYRYEHRAVFAAMSLLINASKPADVITVFEQLQSSGKAEEIGGLSY
                     LNALAQYVPSAGNIRRYAEIVRDRAILRKLVSTSEEISANAFNPKGRPVASILDEAEQ
                     KIFNIGEEGARTRQGFQTMDKLVVELLDRVQEMADNPNDVTGVPTGFYDLDRLTAGLQ
                     GGDLVVLAARPSMGKTALAINIAEHVALNEGLPVAVFSMEMGAAQLAVRIVGSIGRID
                     QSHLRTGKLTDEEWPRLTEAIEKLRTISLHIDESAGLTSGELRANARRLSRQCGQLGL
                     IVVDYLQLMSGSNGSDGENRATELGEISRGLKMLAKELKCPVIALSQLNRSVESRPDK
                     RPMMSDLRESGAIEQDADIIMFIYRDEYYTKDACKEPGVAEVIIAKQRNGPTGVVKLA
                     FLNKITKFESLATGATGDF"
     gene            complement(2861..3313)
                     /gene="rplI"
                     /locus_tag="PLB25_18210"
     CDS             complement(2861..3313)
                     /gene="rplI"
                     /locus_tag="PLB25_18210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012207025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HOE43540.1"
                     /translation="MQIILLDKVVNLGNLGEIVKVKDGYARNFLIPAGRARRATEAAK
                     KEFEVRRVELEKVAAGKLTEAQALGEKLAGTVCKLTQKAGVDGRLFGSVTNANIAEEL
                     TKIGFAVAKAQVRMPNGPIKIVSDSTVSVALHTDVVVDITVSVYGETA"
     gene            complement(3326..>3494)
                     /gene="rpsR"
                     /locus_tag="PLB25_18215"
     CDS             complement(3326..>3494)
                     /gene="rpsR"
                     /locus_tag="PLB25_18215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007830781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HOE43541.1"
                     /translation="DYKDIDTLRDFIAENGKIIPARLTGTRAIFQRQLNTAIKRARFL
                     AMLPYSDQHKI"
BASE COUNT          752 a         1021 c          990 g          731 t
ORIGIN      
        1 accggccata ccgacgcacg cggtcggctg ttttttcaga cccagttact caattacgac
       61 ctgcccacca gccttcccca aggcaaggca gacaaccaga ttctgggtgt cgtcgaagcc
      121 ctgcgcaaac agcatgcccc gcgtgaggtg gtgctggtgt ccaaagacat caacatgcgt
      181 gtcaaggcgc gtgccttggg cttatcgact gacgactacc aaagcgacaa gaccctggaa
      241 gacggcgact tgctgtactc aggggcctac gccctgccga ctgatttctg gagcacccat
      301 ggcagcacgg tggaaagctg gcaacagggt gtccatacct tttaccgcgt cagtgggccg
      361 gtggtgccgc aactgctggt caaccagttt gtctattttg aggcaccggg tgaacccagc
      421 ctgtatgcca aggtgaccga gattcgtggc aaggtggcgg tgtttcggac cctgcgtgac
      481 tttacccatc tgaaaaatgc ggtttggggt attaccaccc gcaaccgcga acaaaacttt
      541 gccatgaacc tgctgctcga ccccgaggtt gactttgtca cgctgaccgg ccaggctggt
      601 accggcaaaa cactgctggc gctggcctct ggtctgaccc aggtgctcga cgagcgtcgc
      661 tacaccgaga tcatcgtcac ccgcgctacc gtcagcgtcg gcgaagatat cggctttttg
      721 cccggcacag aagaagaaaa aatgggtcca tggatgggcg cgctcgacga caacctggaa
      781 gtactgggca aaacggacac cgctgcgggt gaatggggcc gcgccgcgac caatgagctg
      841 attcgaagcc gaatcaagat caagagcatg aatttcatgc gtggccgtac cttcctgaac
      901 aagtacgtga tcattgacga ggcacaaaat ctgacaccca agcaaatgaa gaccctcgtt
      961 acccgggccg ggccgggcac caagatcatc tgcatgggca acattgcgca aattgacacg
     1021 ccgtacctga ccgaaggctc gtcagggctg acctatgcag tggacaagtt caagggctgg
     1081 cagcacagcg gccacatcac gctggcgcgc ggcgaacgct ccaggctggc cgactttgcc
     1141 agcgaggtgc tttaattatg atagctggta gcgctttatc cacaagggct acaggccgat
     1201 ttcattgctc attgaggtga ctgaagccga ctccaccagg cgatcggctc atggggcacc
     1261 tggcgtggcc gtcagaagtc gccggtggca cctgtggcca gtgattcaaa cttggtgatc
     1321 ttgttcagga atgccagctt gacaacacca gtcggaccat tacgctgctt ggcaatgatc
     1381 acctctgcca cgccgggctc cttgcaggca tccttggtgt agtactcgtc acggtaaata
     1441 aacatgataa tgtcggcatc ctgttcgatg gcaccggact cgcgcaggtc gctcatcatc
     1501 gggcgcttgt cggggcgtga ttcgacgctg cggttgagct gcgacagcgc aatgaccggg
     1561 catttgagct ctttggccaa cattttgagg ccgcgcgaaa tttcacccag ttcagtggcg
     1621 cggttttcac catcgctgcc attggagcca ctcatcaatt gcaggtagtc caccacaatc
     1681 agacccaatt ggccgcactg gcgcgaaagg cgccgggcat tggcgcgcag ttcgccggaa
     1741 gtcaggcccg ctgattcgtc aatgtgcagg gaaatggtgc gcaacttctc tatcgcctcg
     1801 gtcagacgcg gccattcctc atccgtcaac ttgccggtgc gcaggtggct ctgatcaatg
     1861 cgaccaatcg agcccacaat gcgcactgcc aattgcgccg cgcccatttc catggaaaac
     1921 accgctaccg gcaagccttc attcaaagcc acatgctcgg caatgttgat ggcaagtgcc
     1981 gtcttgccca tggaaggcct ggccgccagc accaccaggt cgccgccctg caagcccgct
     2041 gtcaggcggt ccaggtcata aaagccggtc ggcacgccgg tcacatcgtt ggggttgtcg
     2101 gccatctcct gaacacgatc aagcagctca accaccagct tgtccatggt ctggaagccc
     2161 tgccgggtgc gggcaccctc ttcgccgatg ttgaaaatct tttgctcggc ttcatccaga
     2221 atggaggcca ccggtcgccc cttgggattg aaggcgttgg cagaaatctc ttcgctggtg
     2281 gacaccagct tgcgcaagat ggcgcggtcc cgcacaattt cagcgtagcg gcgaatattg
     2341 ccagcgctgg gcacatactg cgccaaagcg ttgagataac tcaaaccacc gatttcttca
     2401 gccttgcccg agctttgcag ttgctcaaac accgtgatca catcggccgg ttttgacgcg
     2461 ttgatcaaca agctcatggc agcaaacacc gcgcggtgtt catagcggta aaaatcctgc
     2521 tcggtcagca catcgctgac ccggtcccag gcagcgttgt ccagcaacag gcccccgagc
     2581 acgctggatt cagcctcgat cgaatgcggt ggaatgcgca actgggcgac ctgacggtcc
     2641 agacccgggt catcattgat gacggaaaaa acggcagaca tgggtgttcc ttgaagcgcg
     2701 caatcctaag gcgtgtgaat tcagttgtcg agtgagaggc tggggggaac tgacgtatag
     2761 cctgtgcaca agctgtgcaa atgttcttga tgccagcgca tcaaatcaaa aagccgccag
     2821 ggcttgcacc gaggcggctt gtgcgatgca ggatttttgg ctaggccgtt tcgccgtaaa
     2881 ccgataccgt gatgtccacc accacatcgg tgtgcagcgc cacgctcacc gtgctgtcgc
     2941 tgacgatctt gatggggcca ttgggcatgc gaacctgcgc ctttgccacc gcgaagccga
     3001 ttttggtcag ttcctccgca atgttggcat tggtcacaga gccgaacaaa cggccatcca
     3061 caccagcctt ctgggtcagc ttgcagacgg ttccggcaag cttctcgccc aaagcttgtg
     3121 cttcggtcaa cttgccagcg gccacttttt cgagttcaac gcgacgcact tcaaactctt
     3181 tcttggcagc ttcggtagcg cgacgggcgc ggccggccgg aatcaaaaaa ttacgtgcgt
     3241 aaccgtcttt gaccttgacg atttcaccca ggttaccaag gttcaccacc ttgtcgagca
     3301 gaatgatttg catggggtgt ttcctttaga tcttgtgctg gtcgctgtac ggcaacatgg
     3361 ccaaaaagcg tgcacgcttg atggcagtat tgagctgacg ctggaaaata gcccgggtgc
     3421 cagtcaggcg ggcggggatg atcttgccgt tttcagcgat gaaatcacgc aaggtgtcga
     3481 tgtccttgta gtca
//
LOCUS       DAOSLL010000526         3490 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_23531_length_3490_cov_4.025619, whole genome shotgun sequence.
ACCESSION   DAOSLL010000526 DAOSLL010000000
VERSION     DAOSLL010000526.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3490)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3490
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23531_length_3490_cov_4.025619"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(73..279)
                     /locus_tag="PLB25_18220"
     CDS             complement(73..279)
                     /locus_tag="PLB25_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43542.1"
                     /translation="MKPIKLTPPEQMSARQRAAEITSILSAAIMRTHGSGQAATDQIE
                     RQVGLGFSGDQRVHTNPYQTRSSK"
     gene            441..1562
                     /locus_tag="PLB25_18225"
     CDS             441..1562
                     /locus_tag="PLB25_18225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HOE43543.1"
                     /translation="MLGKISQKLIGYRVKSARVAKGWTQDQLTDGLGLKDRQSVSDIE
                     NGKRSLKPDEMLALADLFDRDIDFFIDPFAVAGEAQFSWRAAPEVPEDSLDGFELKAG
                     QWIGLLRWLREQQDSRSSVLKRALRLSSQSSFEDAQERAESLVAELDLGVIPAEGLID
                     KIERELDIPVLFVDTVDTADGHSISGATCHLEEMGVILINRNENEARRFFDLAHELFH
                     ALTWDAMKPDHRESNSIEERNKVKRIEQLANSFAAALLMPSASLDKLIKRDQLDNIAH
                     LCEVAALLRVAPVTLAWRLFNLKLIGVDTQRSLSQEKQRPSVSGPPKRFSPTFVKMLH
                     EALENGRLSARKAAKAIGLGLAELTELFAQYDLTAPFEL"
     gene            1575..2096
                     /locus_tag="PLB25_18230"
     CDS             1575..2096
                     /locus_tag="PLB25_18230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43544.1"
                     /translation="MPKIRVFADTNVILESFRTGCWAAICNYFAIETVEKCVEETLTG
                     NPGDPRHVAVPAADLKAGLAGQHSVSRKELATLVLSNPSCSTLDDGEKHLFAWLAANK
                     LLPSQGIIVTTADKAALVASNGLGWLDCMTSLEDLARQAGVGRGNRNAFALQYREDWL
                     SSIKTKIRLGIIP"
     gene            2168..>3490
                     /locus_tag="PLB25_18235"
     CDS             2168..>3490
                     /locus_tag="PLB25_18235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43545.1"
                     /translation="MVKKSQKNSKHFVDLINSATLTSISLLARVDKFTFLAVLDTSKP
                     EHLARSELLERIQSVKRDDITIADQEAVRLLQLVRFRTEEMLDYAYGKIEFENHPERG
                     TFDRSTDAMTRMIWLRVNASHIFDQIETIYLTHHFHGHKKFLGFTVRDGDGRDFEWTP
                     EVAKKLHEGVGEILGLDEESKNSCEVIHFEMDAGDESVKRRLHYLVVYHPGKMKLLRQ
                     MKDRRRDLFLFTPALEATLVYDPADNKVHVLSDKKGTAKQLADRFAAIGFEKPLSKQP
                     VDEVSYELAMFKHKVNLMDSKANGAVIQDAWVSALTVTMGHTRHSVTLALANSDNLWR
                     VSDTQFGDRNPLSSCRSILEVKLSFVIRFDGDDDSRALDITVGQRGSCNLLSLPDPRM
                     RKCGEEILTSLGVMKQVKPVKVGADLALFRAEMKLLDLAVDEVDGHLLT"
BASE COUNT          802 a          912 c          985 g          791 t
ORIGIN      
        1 gtgcccacaa gtctggtgtt ggcaggctgg gcagtgcggc cacttgggca gccactgagg
       61 tggtggtgac actcatttgg aactccttgt ttgatagggg ttcgtatgaa cgcgctggtc
      121 accagaaaag ccaagtccaa cttgtctttc tatttggtct gtcgcggcct gaccactgcc
      181 gtgagtacgc ataatggctg ccgacaggat ggatgtgatc tcggctgctc gctggcgcgc
      241 tgacatctgt tctgggggag tgagtttgat aggtttcatg gtttgcaaag ggcatttgaa
      301 agacccagaa atcatgaatc aaatcctcca agacgatggg taacgtaggg caacgccgca
      361 tcttgcaatt ataaaaaagc aagtttttct tgcatttata aaattgcatg tttaaactat
      421 gcgttttgga ggtcactgcc atgcttggaa aaatttcgca gaagcttatc ggctaccgcg
      481 ttaaatccgc acgcgttgcc aaaggctgga cccaagacca acttaccgat ggtttgggct
      541 tgaaagaccg tcagtcagtc tctgatatcg aaaacggtaa gcgctcgctc aaaccagatg
      601 aaatgctggc gcttgcagat ttatttgatc gcgatattga ttttttcatc gacccgttcg
      661 ccgttgctgg ggaggcgcag ttttcctggc gtgccgcgcc ggaggtgccc gaagacagcc
      721 tggacgggtt cgagctcaag gctggtcagt ggatcggcct gcttcgctgg ttgcgcgagc
      781 agcaggacag ccggtcgagt gtgctcaagc gtgccctgcg gctgtcctcg cagtcgtctt
      841 tcgaagacgc acaggagcgc gcggaaagct tggtcgccga acttgatctc ggcgtcattc
      901 cagccgaagg tttgatcgac aagattgagc gcgagttgga cattccggtt ctgttcgtcg
      961 atacggtcga cactgccgat ggtcattcca tctctggcgc tacttgtcac ctggaagaga
     1021 tgggtgtcat cctgatcaac cgcaacgaga atgaagcccg gcgtttcttc gatctggcgc
     1081 acgagctctt tcatgctctg acttgggatg cgatgaagcc ggatcaccgg gaatcaaact
     1141 ctatcgagga acgcaacaag gtcaagcgca ttgagcagtt ggcgaacagt ttcgccgcag
     1201 cgctgctgat gccgagtgcg tccctcgaca agttgatcaa gcgtgaccag cttgacaaca
     1261 ttgcgcacct gtgcgaagtc gctgccctgc tgcgggttgc ccccgtcacg ctggcatggc
     1321 gattgttcaa cctcaagctc atcggtgtcg acacccagcg gagtctttcg caagagaagc
     1381 agcgaccatc ggtgtcaggc ccacccaagc ggttctctcc caccttcgtg aaaatgcttc
     1441 atgaggccct ggagaacgga aggctttcgg ctcgcaaagc cgccaaggcc ataggtcttg
     1501 gactggccga gttgaccgag ctgtttgctc agtacgacct caccgcaccg ttcgagctgt
     1561 gaggtaagca ccgtatgccg aaaatccgag ttttcgcgga caccaatgtc atcctcgaat
     1621 cattccgaac aggctgctgg gcagcgatct gcaactactt cgccatcgag actgtcgaga
     1681 agtgcgtaga ggaaacgctt accggcaatc ctggcgatcc gcgacacgtc gcagtacctg
     1741 ctgccgattt gaaagcaggg cttgccgggc agcactcggt cagcaggaaa gagcttgcaa
     1801 ccctcgtgtt gagcaaccct tcatgcagca cacttgatga tggcgaaaag catctctttg
     1861 cttggctggc tgccaacaag ctgctgccct cccagggcat catagtcacg acagctgaca
     1921 aggctgcttt ggtggcatcg aatggactgg ggtggctcga ttgcatgacc tcgctggaag
     1981 atttggcccg ccaggcaggt gttggacgcg gcaatcgcaa tgcatttgca ttgcagtacc
     2041 gcgaggactg gttgtccagc atcaaaacca agatcaggtt gggaatcatc ccatgacaaa
     2101 agtttgaggc tgtgggcgcg accatcaagg ggaggaaagc cactcagcat tgttcaagga
     2161 gcatcaagtg gtaaaaaaat ctcaaaaaaa cagcaagcat ttcgttgacc tgatcaactc
     2221 ggcgaccctt acatccatat cgctgctggc acgagtcgat aagttcacat ttctggctgt
     2281 gctcgatacg tccaagcctg agcatctggc ccgttccgaa ctgctcgaac gcatccaatc
     2341 agtcaaacgc gacgacatca ccatagcgga tcaggaggcg gtgcgtctac ttcagctggt
     2401 gcgctttcgc actgaagaga tgctggatta cgcctacggg aaaatcgaat tcgaaaatca
     2461 tccggagaga gggacttttg accgatccac cgacgcgatg acccgcatga tctggttgcg
     2521 agtgaatgct tcgcacatct tcgatcagat tgagacgatt tacctcacgc atcatttcca
     2581 cggacacaag aaatttctcg gatttacagt gcgggatggt gacgggcgtg actttgaatg
     2641 gacaccggag gtggcaaaaa aactgcatga gggcgtcggt gagattctcg gtttggacga
     2701 agaatcaaag aatagctgcg aggtgatcca ttttgagatg gacgctggcg acgaatccgt
     2761 caaacggcgg ctgcactatc tcgttgtata tcaccccggc aagatgaagt tgctgcgcca
     2821 gatgaaggat cgcaggcgtg acctgtttct gttcacacct gcgctcgaag ccacactggt
     2881 gtatgacccc gccgacaaca aagtgcatgt gctctccgac aaaaagggta ccgccaagca
     2941 actggcggat cggttcgctg ccatcggttt tgagaagccc ctttcaaaac agccagtgga
     3001 cgaggtcagc tacgagctgg caatgttcaa acataaagtg aatctcatgg attcgaaggc
     3061 caacggcgct gtcatccagg acgcttgggt ttcagcgttg accgtgacca tgggtcatac
     3121 acgccacagc gtaaccctgg cgttggcgaa cagcgacaat ttatggcgtg tctctgatac
     3181 gcaatttggc gaccgcaacc cgctatcaag ctgtcgctcc atcctcgaag tgaagttgtc
     3241 gttcgtgatc cgttttgacg gcgatgacga tagccgagcg cttgatatca cagtggggca
     3301 gcgcggctcc tgtaacttgt tgtcgctgcc cgatcctcgg atgcgcaaat gcggcgagga
     3361 gatattgacc tcactcggtg tcatgaagca ggtgaaaccg gtcaaagtcg gagctgactt
     3421 ggcgttgttc cgggccgaga tgaaacttct tgacctcgcc gttgatgaag tcgatggtca
     3481 cctgctgacc
//
LOCUS       DAOSLL010000527         3475 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_23651_length_3475_cov_4.193860, whole genome shotgun sequence.
ACCESSION   DAOSLL010000527 DAOSLL010000000
VERSION     DAOSLL010000527.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23651_length_3475_cov_4.193860"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="PLB25_18240"
     CDS             complement(<1..603)
                     /locus_tag="PLB25_18240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43546.1"
                     /translation="MSDSQIGNFGKFVPGFEFLQNLAKSASQTIPQMPNMSNWIAPTM
                     SVEDLEKRINELKAVHFWLDQNTRALGATVQALEVQKMTLATLKGMNFNLGDVANAFK
                     LKAADSVYSGVQKVTEKAASTARTVSDVAEQTEGIAKKVGDAAKPAAAAAMAGLVDPM
                     QLWTSLTQQFQTIAASAMKEATKTTAMDAAMDMGKNLAKEM"
     gene            complement(786..1250)
                     /gene="nikR"
                     /locus_tag="PLB25_18245"
     CDS             complement(786..1250)
                     /gene="nikR"
                     /locus_tag="PLB25_18245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HOE43547.1"
                     /translation="MERFTISLDDTLARQFDELIGQRGYGNRSEAVRDLIRAAIEADR
                     QRQPPTGNCVGMLSYTYDHHERLLADRLTGLQHNHHDLTVASMHAHLDHTQCVESVIL
                     KGSTADVRQFADALMAECGVHHGHLNLIALEAHQPHAHGQDGEPHIHYSPAS"
     gene            complement(1468..2070)
                     /locus_tag="PLB25_18250"
     CDS             complement(1468..2070)
                     /locus_tag="PLB25_18250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HOE43548.1"
                     /translation="MKFISVNVARLASLAVLLLATGAAQAHTGHGTSGVLEGLVHPFG
                     LDHLLAMVAVGVWSVSALPASQAWRGPAMFMLALVVSAALGAAGVSVPFLEQLISLSV
                     VLFGLMLVFSRSQIPAAFGLCLVALAASLHGVAHGAETPETGFATYAAGFLLTTAALH
                     FGGVLAGLGVRRWLASRASLAVSGFGILCGGAGLYLFSQI"
     gene            2257..2766
                     /locus_tag="PLB25_18255"
     CDS             2257..2766
                     /locus_tag="PLB25_18255"
                     /inference="COORDINATES: protein motif:HMM:NF022458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2844 domain-containing protein"
                     /protein_id="HOE43549.1"
                     /translation="MFIFHGVLSKRLAALATLALATSGAMAGLGGAPLTASALGADAG
                     AVKLRANEAMKSSAASGSAGLYTLNRLQLASGTTITEYVNKSGVVFAVSWLGPVLPNM
                     SHMLGDHFPVFKSESDRLRQAGKRGGPVSLNAAGLVVVSSGRMGHFSGFAYKDALIPA
                     GVNADDLLN"
     gene            2789..>3475
                     /locus_tag="PLB25_18260"
     CDS             2789..>3475
                     /locus_tag="PLB25_18260"
                     /inference="COORDINATES: protein motif:HMM:NF023352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3443 family protein"
                     /protein_id="HOE43550.1"
                     /translation="MKLFQSAPWLRAALPVLLATLMTACGGGGSGDSAPVTPPVSPPI
                     VQPVPDNTLALTVDAGPAGGGRNINLLYASVTVCQPNSTSQCKTIDHVLVDTGSTGLR
                     LLSSALDNAVVLNPILSSGSLPLLNCAQFVDGSYAWGPVVSADVVLGSKVARGVPIQI
                     IADPVYAAAAGQCAAGNSVIDTASSLGANGILGVGLFLQDCGAGCVSTTGNGYYYTCT
                     DSNCRTSKGVA"
BASE COUNT          727 a          984 c         1029 g          735 t
ORIGIN      
        1 catttccttg gccaggtttt tacccatgtc catggcggca tccatggcgg tggtcttggt
       61 ggcttctttc atggcgctag cggcaatggt ttgaaactgc tgggtcagtg atgtccacag
      121 ttgcatcggg tccaccagtc cggccatcgc tgcggcggcg gcaggcttgg cggcatcgcc
      181 aacctttttg gcgattcctt cggtctgctc ggccacgtcg gacacggtcc gggccgtcga
      241 ggcggccttt tcggttacct tttgcacacc ggaataaacc gagtcggctg ccttgagctt
      301 gaaggcattg gccacatcgc ccaaattgaa attcatgccc ttcagggtgg ccagcgtcat
      361 tttttgcact tccagcgcct gcaccgtggc tcccagcgcg cgagtattct gatcaagcca
      421 gaaatgaacc gccttgagct cgttaatgcg cttttccaga tcttcgacac tcatggtcgg
      481 cgcaatccag ttagacatat tgggcatctg aggaatggtt tgagaggcac ttttggccag
      541 gttttgcaaa aactcaaagc ctggtacgaa tttgccgaaa ttaccgattt gtgaatcgct
      601 catgtgtgtc tccgttaatg gggttaagtg ctgaacaagt aacagcttaa cccaatgtgg
      661 gcatcatggc acgctggcat ttgccgagcc ggcattggcc tggccgacat ccactgacca
      721 cagtctggtg cggatcaatg cgcccccgga caaaagcatg cggcaccgaa ccggccaact
      781 cggactcagg atgccgggct gtaatggata tgcggctcgc catcctgccc atgagcgtgc
      841 ggctggtggg cttccagcgc gatcaggttc aggtgaccgt gatgcacacc acactcagcc
      901 atcagcgcat cggcaaactg gcgaacatct gcggtcgagc ccttcaaaat aaccgactcg
      961 acacactgcg tgtgatccag atgcgcatgc attgatgcta ccgtcagatc atgatggttg
     1021 tgctgcagcc cggtcaggcg atcagccagc agacgttcat ggtggtcata ggtgtaagac
     1081 agcatgccga cacagttgcc tgtgggtggc tggcgctggc ggtcggcctc gatggcagca
     1141 cgaatcaggt cacgcaccgc ttctgaccgg ttgccataac cgcgctggcc gatcagttca
     1201 tcaaactgcc gcgcgagcgt gtcgtcgagc gaaatagtga agcgttccat tgaccatctt
     1261 ctaagtaaaa aagacctcta gcccttgtca atactgcgct gacagctatc tttattgaat
     1321 caagtggggg cccgcaaagg catatgacag caggccgacg ggcgatcaac agcgaccttt
     1381 gcccagtcag gcgaggctcg ctgccatgct gccatgctgc cgatcaacgg cagtacccat
     1441 atgatgcgct gcgaagacag tcaaacctca tatctggctg aacaggtaaa ggccggcacc
     1501 accacacaaa atgccaaagc ccgagaccgc caaacttgcc ctgctggcca accagcggcg
     1561 aacacccagc cctgccagta ccccaccaaa atgcagtgcg gcggtggtca gcaaaaagcc
     1621 tgcggcgtag gtggcaaacc cggtttcagg tgtttcagcg ccgtgcgcaa caccatgcag
     1681 cgatgcggcc aaggccacca ggcacaggcc aaaagccgct ggtatctgac tgcgtgaaaa
     1741 caccagcatc aagccgaaca gcaccacact gagagaaatc agttgttcaa ggaacggcac
     1801 cgaaaccccg gcagcaccga gtgccgcgct gacaaccagt gccagcatga acatggctgg
     1861 cccgcgccag gcctggctgg cgggcaaggc cgaaaccgac cagacaccga ccgccaccat
     1921 ggccagcaga tggtccagcc caaaaggatg aaccagcccc tccaaaacac ccgaggtgcc
     1981 gtggccggta tgcgcttgtg ccgctccggt ggcaagcagc agaacggcaa ggctggcaag
     2041 acgtgcgacg ttgaccgaga tgaatttcat ggttttctcc tgggttgggt aagaactatg
     2101 gcgcggcctg agtttacgca tattttttta aaatttgtct taccatgcct gacagctatg
     2161 cgcccatgat gtgaagcctt taagccaatg aatcttggct tgggtatgat gcttcgaaat
     2221 ggcggacaag tacctgaaac cagtatgagg ttaatgatgt ttatttttca tggggtattg
     2281 agcaagcgat tggctgcgct ggcaacgctg gcactggcaa cgtctggggc tatggctggc
     2341 ttgggcggtg cgccgttgac tgcgtcagcc cttggtgctg atgcgggtgc cgtcaagttg
     2401 cgggccaatg aggcgatgaa atcaagcgca gccagcggct cggctgggct gtatacgctc
     2461 aatcgtttgc agttggccag tggaacgacc atcaccgagt atgtgaacaa gtccggtgtg
     2521 gtgtttgcgg tgagttggct gggaccggtt ttgccgaaca tgagccacat gctgggcgac
     2581 cattttccag tgtttaaaag tgaatcggat cgcttgcgcc aggcaggcaa gcgtggtggt
     2641 ccggtcagtc tcaacgctgc cggtctggtg gtcgtgtcaa gtgggcgcat gggccatttc
     2701 tctggttttg cctataagga cgcgctgatc cctgccgggg tcaatgcaga tgatttgttg
     2761 aattgacgca ccaccccagg gccctaacat gaagctattt caatctgcac cctggctgcg
     2821 cgctgcgctg ccggttttgc tggcgacgct gatgaccgct tgcggcggcg gtggttcggg
     2881 cgacagcgcg cccgttaccc cacccgttag cccgccgatc gtgcaaccgg tgccggacaa
     2941 caccctggca ctgacggtcg atgccggccc ggcgggcggt ggtcgcaaca tcaacctgct
     3001 atacgccagt gtgacggtgt gccagccaaa cagtacaagc caatgcaaga ccattgacca
     3061 tgtgctggtg gataccggct caaccggttt gcgcctgctc tcgtcagcat tggacaacgc
     3121 ggtggttttg aatccgattt tgagcagcgg cagtttgcca ttgctcaact gtgcccaatt
     3181 tgtcgatggc agttacgcct gggggccggt ggtcagcgcg gacgtggtgc tgggcagcaa
     3241 ggtcgcccgc ggtgtgccga tacagatcat tgctgatccg gtttacgcgg cagcggctgg
     3301 ccaatgtgcg gctggcaatt cggttattga tacggccagt tcgctcggcg ccaacgggat
     3361 tttgggggtc ggactgttcc tgcaggactg cggcgccggg tgcgtcagca caactggcaa
     3421 cggctattac tacacctgca ccgacagcaa ttgccgaacc agcaagggcg tcgct
//
LOCUS       DAOSLL010000528         3444 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_23933_length_3444_cov_3.943346, whole genome shotgun sequence.
ACCESSION   DAOSLL010000528 DAOSLL010000000
VERSION     DAOSLL010000528.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3444)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23933_length_3444_cov_3.943346"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /locus_tag="PLB25_18265"
     CDS             <1..1534
                     /locus_tag="PLB25_18265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007869513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HOE43551.1"
                     /translation="FMNIAEQMGLQLQNTAYSVNIKERLDFSCALFDAAGNLIANAPH
                     MPVHLGSMGESIKTVIAENTGQMSPGDVYVLNDPYHGGTHLPDITVITPVYLAASPEP
                     MFFVGSRGHHADIGGITPGSMPPFSTNIEQEGVQIHNFKLVDRGLLQEAGMLALLQGG
                     NYPSRNPQQNMADLKAQIAANEKGLQELHKMVDQFGLKVVQAYMRHVQDNAEEAVRRV
                     ITRLKDGQFTLPLDNGAQICVAIRVDATARRAEIDFTGTSAQQGNNFNAPTAVCMAAV
                     LYVFRSLVGDDIPLNAGCLKPLKVIIPPGSMLNPNPPAAVVAGNVETSSCITNALYGA
                     LGSMAAGQCTMNNFTFGNAQHQYYETISGGSGAGALFDADSRVTGGYHGCSVVQTHMT
                     NSRLTDPEVLELRFPVRLESYQIRHGSGGAGQWRGGDGGVRRLRFLEPMTASILSNGR
                     LHGAFGVAGGQPGQVGINRVLRQDGQVETLGHIGQAEMAAGDVFEICTPGGGGYGPPE
                     PV"
     gene            1674..1952
                     /locus_tag="PLB25_18270"
     CDS             1674..1952
                     /locus_tag="PLB25_18270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HOE43552.1"
                     /translation="MTDVTYRLEISGRVQGVYYRGSMVSQAHALGLRGWVRNRLDGSV
                     EAMVQGDAVAVSRLIEWSRRGPGGALVTGVNVHPAEGDFTGFKQVQTR"
     gene            complement(2545..3291)
                     /gene="fabG"
                     /locus_tag="PLB25_18275"
     CDS             complement(2545..3291)
                     /gene="fabG"
                     /locus_tag="PLB25_18275"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HOE43553.1"
                     /translation="MTARLSNKVSIITGAAQGIGLATALKFASEGAIVIVCDVGQAGV
                     DEAVAQCRALGATAEGFVMDVTRRAVVDEVVARVKARFGRIDVLVNNAGITQDARLQT
                     MTLEQFDRVIDVNLRGVFHCSQAVADGMVAQGSGVILNASSVVGLHGNFGQTNYAATK
                     FGVIGFTKTWCRELGPKGVRVNAVAPGFIGTPMVAAMPDKVLADLSAKVHLRRVGKPE
                     EVANVYAFLASDEASYINGEVIEVSGGMTL"
BASE COUNT          698 a         1028 c         1029 g          689 t
ORIGIN      
        1 gttcatgaac attgccgagc aaatggggct gcaactgcag aacaccgcct actcggtcaa
       61 catcaaggag cgccttgact tcagttgtgc actgtttgac gcagcaggca acctgatcgc
      121 caacgcgccc cacatgccgg tgcatctggg cagcatgggc gaaagcatca agacggtgat
      181 tgccgagaac accggccaga tgtcgccggg tgatgtctat gtgctcaacg acccctacca
      241 tgggggcacg catctgccag acatcacggt gatcacgccg gtgtatttgg cggcatcgcc
      301 agagcccatg ttttttgtcg gcagccgtgg ccaccatgct gacattggtg gcatcacgcc
      361 gggttcgatg ccaccttttt ccaccaacat tgaacaagag ggcgtgcaga ttcacaactt
      421 caagctggta gatcgtggcc tgctgcaaga ggctggcatg ctggcactgt tgcaaggtgg
      481 caactacccc agccgcaacc cgcagcagaa catggccgac ctgaaagcgc aaattgccgc
      541 caatgaaaaa ggcctgcagg agttgcacaa gatggtcgat caatttggcc tgaaggtggt
      601 gcaagcctat atgcgccatg tgcaggacaa cgccgaagaa gcggtgcgcc gcgtcatcac
      661 ccgcctgaag gacggccagt tcacgctgcc actggacaac ggcgcgcaga tttgcgtggc
      721 gattcgggtg gatgccacag cgcgccgtgc cgagattgac tttaccggca ccagtgccca
      781 gcagggcaac aacttcaacg cgccgacagc ggtctgcatg gcggcggtgc tgtatgtgtt
      841 tcgcagcctg gtgggcgacg acatcccact caatgccggt tgcctgaagc cgctcaaggt
      901 catcatccca cccggctcga tgcttaaccc gaacccgcca gccgccgttg tggcgggcaa
      961 tgtggaaacc tccagttgca tcaccaatgc gctgtacggc gcacttggca gcatggcggc
     1021 cggtcagtgc accatgaaca acttcacctt tggcaacgca cagcatcaat actacgagac
     1081 catttcgggt ggttccgggg cgggcgcgct gttcgatgcc gacagccggg tgacaggcgg
     1141 ctaccacggc tgcagtgtgg tgcaaaccca catgaccaat tcacgcctga ccgaccccga
     1201 ggtgcttgaa ttgcgctttc cggtgcggct ggaaagttac cagattcgcc acggctccgg
     1261 cggggccggg caatggcgcg gcggtgatgg cggtgtgcgc cggttgcgtt ttctggagcc
     1321 gatgacggcc agcattttgt ccaacggccg cctgcatggc gcctttggcg tggccggtgg
     1381 ccagccagga caggtcggca tcaaccgcgt gctgcgccag gatggccagg ttgagaccct
     1441 cggtcatatc gggcaggccg agatggcagc gggcgatgtg tttgaaatct gcacgcccgg
     1501 cggtggtggt tacggcccgc ccgagccggt ttaaggctgc cagtgcgggt gtcgtacaac
     1561 ctggttcgcc agagtgaggc cgacgatgcc gacggcctgc tggcgctgcc ttgccgttga
     1621 cgcactgtgg taacttctca ctgctattga tgtcaaaatt gaaccacaca accatgacag
     1681 atgtaaccta ccgacttgaa atttcagggc gagtccaggg tgtctattac cggggctcaa
     1741 tggtctctca ggctcatgca ctgggtctga gaggatgggt gcgaaatcgc ctggacggtt
     1801 cggtagaagc catggtgcag ggcgacgccg tcgcggtcag cagactcatc gaatggtccc
     1861 ggcgcggccc cggcggtgcc ttggtcactg gcgtcaatgt ccatccggct gagggggact
     1921 tcaccggctt caaacaagtc cagaccaggt aaagcttgat gaattcccag atttcccagg
     1981 acggttcatg gactgatcgc agaaaaggct atcaatattg cggggttctg ttttcagaaa
     2041 atggtaccga gttgaatctg tcgggggtgc agtatcgtga gctgcctttt tcagcgagtg
     2101 caaacaaacg ctttcgggtc tggcttgcgg tgtgcctgct gtttgttttt cctggcattc
     2161 cattgctggc ttactggggc ttgaaccagc acctgctctg gattggtgcc attttttgca
     2221 tcctctcgta ctggttgcat gggcgcattc actgctgccc cggatgtggc ggaaagtcca
     2281 ggccactgag cacgccttac atgaactcgc cagtactcta tctttgcagc cggtgccata
     2341 cgttttttga acacggacag gtcgatggcg gctggccctg gaagtaactt ccgcctggtc
     2401 agcaaagctt gcccgtcggc atcgctactt tggcacaaca ccaccttcaa gctgccagaa
     2461 agcccgaagc tgcaacaagc aggcagggcc gcgcatcgga catcgccctc atcagtccag
     2521 tcagacccgg cgatcttcag ccgatcaaag cgtcatgccg cctgatacct ctatcacctc
     2581 gccattgatg tagctggcct cgtcactggc caggaaggca tagacattgg cgacttcttc
     2641 gggtttgcca acgcggcgca gatgcacttt ggcgctcagg tcggccagca ccttgtcggg
     2701 catggcagcg accatcggcg tgccaataaa gccgggcgcc accgcattga cgcgcacgcc
     2761 tttggggccg agttcgcggc accaggtctt ggtaaagcca atcacgccaa acttggtggc
     2821 ggcgtaattg gtctggccaa aattgccatg cagaccgacc accgaactgg cgttcagaat
     2881 cacgccgctg ccctgggcca ccatgccatc ggccaccgcc tggctgcagt gaaagacgcc
     2941 gcgcaagttc acatcaatga cacggtcaaa ctgctcaagc gtcatggttt gcaggcgcgc
     3001 gtcctgggtg atgccggcgt tgttgaccag cacgtcaatg cggccaaacc tggccttgac
     3061 ccgggccacc acctcatcga ccacggcgcg ccgggtcacg tccatgacaa aaccttcagc
     3121 cgtggcgccc agggcacggc attgcgccac ggcttcgtca acgccagcct gcccgacatc
     3181 acaaacgatg acgatggcac cctcgctggc gaacttgagg gcagtggcca ggccaatccc
     3241 ctgcgctgca ccggtgatga tggagacctt gttgctaaga cgtgcagtca tgacgtttcc
     3301 agtctttgat ggtaaaaccc agcggattct aggcatcagg ctttgcaaga acttgacaaa
     3361 tgccgggcac gctgctgcaa aacctggcgt tgaacccctt agccggacga gccggaatcg
     3421 atgagccttc gtcgcgaggg cgcg
//
LOCUS       DAOSLL010000529         3433 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24029_length_3433_cov_4.318532, whole genome shotgun sequence.
ACCESSION   DAOSLL010000529 DAOSLL010000000
VERSION     DAOSLL010000529.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24029_length_3433_cov_4.318532"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="PLB25_18280"
     CDS             <1..995
                     /locus_tag="PLB25_18280"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: protein motif:HMM:NF017873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050151 - oleate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HOE43554.1"
                     /translation="NFHSFILPALRFMQKKGVNFHYNARISDVDFKVDAGGKQRWIER
                     IHLETGSGKPKPAIEVRDTDLVFLTIGSMVSNASFGAHDRPVDKPAVGSQNLGGAWTL
                     WTKIAAKQPDFGRPDQFLRDISQSTMMTFTVTLRGGLFQRKFEWLVERQMGRQSPLPL
                     TASPWILHLHTYRQPLFPDQPRDTCVIWVTALGSDHLGTFVKKTMSECTGREILEELL
                     GHLQFDQDDVEKNEMLDTSTCIPCMLPYCISQFLSRARGDRPAVVPSGSKNFAFLGQF
                     VEIPHGIVFTTEYSVLGAIHAVKEFVKPSLVVPPMYFGQYHPLTSIAVVRALAH"
     gene            complement(992..1138)
                     /locus_tag="PLB25_18285"
     CDS             complement(992..1138)
                     /locus_tag="PLB25_18285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43555.1"
                     /translation="MLTERTSVFMLALRLVQRNLLRSFLTIPGMVIGVSAVITMVTLG
                     NGAI"
     gene            complement(2073..>3433)
                     /locus_tag="PLB25_18290"
     CDS             complement(2073..>3433)
                     /locus_tag="PLB25_18290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE43556.1"
                     /translation="IRHVTGKLTDVHYNASLYRDAKGNVLGVFAAARDITESKRMELA
                     LEVRNIELETATAVAEEASLAKSNFLSSMSHELRTPLNAILGFAQLIESNVSQPTPSQ
                     KRSLDQILSAGWYLLELVNEILDLAQIESGKAVMTREAVPLEHIMSECQELVEPLAQK
                     RGINISFPRMERPHWVEADRNRTRQVLINLLHNAIKYNKPGGMVSMACTLVPPNSIRI
                     SVRDTGPGLSPEQLGQLFQPFNRLGRESGGEQGTGIGLVVVKRLVELMGGVIDVESQV
                     GVGSVFWIELNLTTPPMLTAGETEQAAPNSPELKDGTVRRTVLYVEDNPANLALVEEL
                     LTRRPDLRLISAADGNLGVEYARMYLPDTILMDIHLPGISGIEAMKTLRADPSTADIP
                     VIALSAHALPTEIIKTMEAGFFNYITKPIKLKEFMAALDVALEFSSSKSSDAAVQAST
                     TG"
BASE COUNT          684 a         1019 c          961 g          769 t
ORIGIN      
        1 ccaacttcca ttcttttatt ctgccggctc tgcgcttcat gcagaagaaa ggcgtgaact
       61 tccattacaa cgccaggatc tcggacgtcg acttcaaggt cgatgccggt ggcaaacaga
      121 ggtggattga gcgaatccac ctcgagaccg ggagcggcaa gcccaagccc gccatcgagg
      181 tgcgcgacac cgacctcgtt ttcctgacca tcggctccat ggtcagcaac gcctcgttcg
      241 gcgcccatga ccgcccggtg gacaagcccg ccgtcggcag ccagaacctt ggtggcgcat
      301 ggacgctatg gacgaagatt gccgccaagc agcccgactt cggccgccct gaccagttct
      361 tgcgtgacat ctcgcagtcc acgatgatga ccttcaccgt caccttgcgc ggcggattgt
      421 tccagcgcaa gttcgagtgg ttggtggagc gccagatggg tcgtcagagc ccgctgcccc
      481 tcaccgcctc cccatggatc ctgcacctcc acacgtaccg ccagcccttg tttcccgatc
      541 agccccgcga cacgtgcgtc atctgggtga ccgcgctggg tagcgaccac cttggcacgt
      601 tcgtgaagaa gaccatgtcc gagtgcacag gtcgcgagat cctggaagag ttgttgggtc
      661 acctgcagtt tgaccaggac gacgtggaga agaacgagat gctcgacacc tccacctgca
      721 ttccctgcat gctgccctac tgcatcagcc agtttctcag cagagcccgg ggcgaccggc
      781 ctgcggtggt gccatcgggt tcaaagaact tcgcgttcct tgggcagttt gtagagattc
      841 cccacgggat cgtgttcaca acggagtaca gcgtattggg cgcgattcat gcagttaaag
      901 aattcgtcaa gccgtcgctt gtggtgccgc caatgtactt cgggcagtac cacccgctca
      961 cctccattgc agttgtacgt gctttggccc actagatggc accattgccc agcgtcacca
     1021 tggtgatcac cgcgctgacc ccaatcacca tccccggaat ggtcaggaac gagcgcagca
     1081 gattgcgctg caccaagcgc agggcgagca tgaacacgct ggtgcgttca gtcagcactg
     1141 cggagtgccg taaggggcaa tgcctccagc ttgaaaagca ccattttgtg tttgctaccg
     1201 ccagcccaaa tgcatctgag gctgccatgt ctgacttgcc gcccaacgcc tgcggtgcca
     1261 gtccaggcgt tttcggtggc tcacgcgcct gtcggcagcg catggccagg gcacagttga
     1321 gcaatttata agaaatcagg ctctagccca tactgtctgt gcgctacatg ctactgtaaa
     1381 tatagctgtt tgaggttgcg ccaagttgcc aggccgctgc atcgacaccc gcctggtcat
     1441 tgctttccct gctggcccag tcgccggatg gccggtcatc acatcacctg tcggcaagtc
     1501 cggcagcgtg tgtggatttg tgcgctgttg cgcaggggtg ctacacaaaa accactgccg
     1561 accgtcagac ctgcggcgcg aagggtgggt tttgacccac tgcagttaca gcggctattc
     1621 aaatttgcgc ctgaaagcca acatccagcc gccttcgaca tcgcggtgtc aatgtccgct
     1681 aacgaggtgg ctcattttca aagtagtcgt ctcgaactgc ccaggtgctg tcattggaat
     1741 ccctgacccc gttgctgccg ttggggaacc cgaattctcg ataccggtcg ttcagtctga
     1801 aaaaacggaa tatgactttc tccggttcct gtggggcaga agctgtctta gccttttttg
     1861 gagcagatga ctgttttggg ctggaagcag tctccgacca gaacgatggt actgagtcca
     1921 gagtcaattc cagaactcag tttcagtcac cgcaaagctg acgcacgaaa gcacttcatt
     1981 gccgctcagt cgatggacag ggtcgtcgcg aagccttcac tcagcttggg actccaattt
     2041 gcgtagaagc tccccagact gtgatgattt tgtcagcctg ttgtcgatgc ctgcacagcc
     2101 gcatcggacg acttgctgct ggagaactct aatgcaacgt ccaacgcggc catgaactcc
     2161 ttgagcttga tgggtttggt aatgtagttg aagaatccgg cttccatggt cttgatgatc
     2221 tcggtcggca aggcatgggc actgagcgcg ataacgggaa tatccgccgt cgacgggtct
     2281 gctcgcaagg tcttcatggc ttcgatcccg ctgatgccgg gcaggtggat gtccatcaga
     2341 atggtatccg gcaaatacat gcgtgcgtac tcgacgccaa gattaccgtc tgctgcggaa
     2401 attaggcgca gatcggggcg gcgcgtcagg agttcttcca ccagtgccag gttggctgga
     2461 ttgtcctcca cataaagaac ggtgcgtcgc acggtgccgt ccttcagttc cgggctattc
     2521 ggcgccgcct gttcggtttc gccagccgta agcatcggtg gagttgtgag gttcaattcg
     2581 atccagaaaa cgcttcccac cccaacctgg ctttcaacgt caatgactcc ccccatcagt
     2641 tccaccagcc gcttgaccac aacgaggcca atgcccgtgc cttgttcccc cccgctttcc
     2701 ctgccaagac ggttgaacgg ttgaaacagt tggccaagct gctctgggct caagcctggc
     2761 cccgtatcgc ggacgctgat acggatcgaa ttcgggggaa ccagggtgca cgccatgctg
     2821 accatgcctc ctggcttgtt gtatttgatc gcgttgtgaa gcaggttgat cagaacctgt
     2881 ctggtccggt tacggtcggc ttcaacccag tggggccttt ccatgcgcgg aaagcttatg
     2941 ttgatgccgc gcttttgtgc cagcggctcg accagctcct ggcactcgct catgatgtgc
     3001 tccagaggca cggcttctcg cgtcatcacc gctttgcctg actcgatctg cgccagatcg
     3061 agaatttcgt tgaccaactc aagcaaatac catccagccg agagaatctg atccagactg
     3121 cgcttttggg aaggtgtcgg ctgggaaaca ttggactcga tgagctgggc gaatcccagg
     3181 atcgcattga gtggcgtgcg caactcgtga ctcatactgg aaaggaaatt cgacttcgcc
     3241 aaactggctt cttcggccac agcggtggct gtttccaact cgatattcct tacctccagc
     3301 gcgagctcca tccgtttgga ttcggtgatg tcacgggctg cggcgaacac accaagcaca
     3361 tttcccttgg catcacggta caggctggcg ttgtagtgga cgtcggtgag cttgccagtg
     3421 acatggcgaa tcg
//
LOCUS       DAOSLL010000530         3430 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24056_length_3430_cov_3.722074, whole genome shotgun sequence.
ACCESSION   DAOSLL010000530 DAOSLL010000000
VERSION     DAOSLL010000530.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3430
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24056_length_3430_cov_3.722074"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="PLB25_18295"
     CDS             <1..1229
                     /locus_tag="PLB25_18295"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE43557.1"
                     /translation="SSNWIYRKKDEDKVHEGEADFTLLFPRSGVLAVEVKGGGVALDA
                     SAGEWVSVDRNGRRNAIKNPFKQASDERHALLDQILGHVAWRRWPGKRLTLGHAVMLP
                     DVRDATPLLGPDRKKEFMGVHGDMQALDTWVHRVMRFWHQGGEDELGSTGVQLIKDIL
                     CQSIEVLPALKSLVDDAEQQRIKLTANQAKVFRTIGGRRRAIVSGGAGTGKTLLAAQR
                     ARTLADTGLNVLLVCYNRPLADSLALGMSGVTGLTVMGYHQFCEAMIRQAKALGSDVL
                     REAQDAYPSDSTQQEFDMQYPVALALAAEVVPQRFDAILVDEAQDFGDEYWLGLELLL
                     KDPTSSYFYVFIDENQRLYERRSTIPVQEEPFYLTYNCRNTAPIHGLAYRYYSGDAVD
                     PPDLLGPDIAWVSTTA"
     gene            1574..2758
                     /locus_tag="PLB25_18300"
     CDS             1574..2758
                     /locus_tag="PLB25_18300"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOE43558.1"
                     /translation="MPVVQFTPAFIASGLVCPTGKKKIEFSVADEPGLFVECRDSVTA
                     TPVWYLRLKNAQGTNTYKKLGTVKDLSLAQARKLAKQLRAEHTIVKQSVAAPTAAPQE
                     LTLARFFDEQYLPHAKVHKRSYAKDITLYRLRIAPKFGHLPLSAINRREVQKFHNDLL
                     SEGLSPASCNHHVVLFRRMLNLACSWELLEKNVLRGIPLLPLDNFRENFLSAEETNRL
                     VEVLTTDANKTVCAILLFLLNTGARRMEAIQAKWVDVDLENRLWKIPAANNKSKRPKS
                     LPLNDSAMQILQGLESKGTNPYLFPNPQTGLPYSGIMRVWYRLRKKAGLDPDMRVHDL
                     RACLAERLLSAGQSLFLVQKLLGHQDPRTTLRYARLSSKALLDASNIASVALPSVPKQ
                     VA"
     gene            2890..3150
                     /locus_tag="PLB25_18305"
     CDS             2890..3150
                     /locus_tag="PLB25_18305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HOE43559.1"
                     /translation="MPTSVALSPHFESFVKQQVSTGRFNNVSEVVRAGLRLLEDQEQL
                     KSLKVQELRSAVEAGAASGAGVNANEVFDRLERKYRNAADAA"
     gene            3140..3430
                     /locus_tag="PLB25_18310"
     CDS             3140..3430
                     /locus_tag="PLB25_18310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019562322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HOE43560.1"
                     /translation="MQLELSPLAETDLEAIGDYIAIDSQGNALRFIQELRDQCRKITK
                     APMAYVARPELGEGLRSCAHGRYVIFFRPGVEVVRVERILHSALDIRSELMP"
BASE COUNT          748 a          897 c          997 g          788 t
ORIGIN      
        1 acagctccaa ctggatttat cggaagaagg acgaggacaa ggtccacgaa ggcgaggccg
       61 atttcacctt gctctttcca cggtccggag tgttggcagt ggaggtcaag ggcggcggtg
      121 tggctttgga tgcctctgcc ggggaatggg tttcggtaga cagaaacggg cgccgcaacg
      181 ccatcaagaa tccgttcaag caggcatccg atgagcgaca tgcccttttg gaccagattc
      241 tgggacatgt cgcctggagg cgttggcctg gcaagcggtt gactctgggg catgcggtca
      301 tgctgccaga tgtgcgcgat gctacgccgc tattgggccc tgaccgaaaa aaggaattca
      361 tgggcgtgca tggtgacatg caggctctgg acacctgggt gcatcgtgtc atgaggtttt
      421 ggcatcaggg gggagaggat gagctgggct ccaccggcgt tcagcttatc aaggacatcc
      481 tgtgccaatc gattgaggtc ttgccagcgc tgaagtcact ggttgacgat gcagagcaac
      541 aacgcatcaa gctcaccgca aaccaggcca aagtgtttcg cactatcggc ggcagacgca
      601 gggctattgt ttctgggggg gcagggaccg gcaagacact tctggccgca caaagggccc
      661 gtaccctggc cgatacaggt ttaaatgtcc tgctggtttg ctacaaccgc ccactggccg
      721 attcactcgc tttggggatg agtggcgtta ccggattgac ggtaatgggc taccaccaat
      781 tttgcgaagc catgattcgc caagccaaag cgctgggctc agacgtactg cgagaggcgc
      841 aggatgccta tcccagtgac tcgacgcaac aggaattcga catgcagtac ccggtcgcgc
      901 tggctttggc ggcggaggtg gtgccacaga ggttcgacgc cattctggtc gatgaagcgc
      961 aggacttcgg cgatgaatat tggctggggc tggagttgct gctcaaagac cccacgtcga
     1021 gctactttta cgtattcatc gatgagaacc agcgtttgta tgagcgccgc tccaccattc
     1081 cggtccagga ggagcccttt tatctgactt acaactgcag aaacacggca cccattcatg
     1141 ggttggcata ccgctattac agtggcgacg cggtcgaccc gccggacctc ttgggcccgg
     1201 atatcgcgtg ggtatctacg acggcataag gcgtgccgta gggatgtgcg tggcttactt
     1261 ggtgaatagc ggcgctctac cgctgaagtt gcatgtcacg aagtcaggca aaggcccaaa
     1321 gcgatacctg atggattcaa cgcggctatc agtgacgtcg cttggggagg ccgtttcgta
     1381 ttgatggcct gtagccctgc ccctttgccg gggtggaggc tatgcggtcg aatctttaac
     1441 tcggattaca gatttgtgcg taagcctatg acctatatcg aaattttgat gaatgtgccg
     1501 cttttgggcg gtgttcgcag cggtgcgcct atggtgtgct gccttattaa ccaatattgc
     1561 caaggagcct gatatgccgg tggttcaatt cacaccagca tttattgcat ccggcttggt
     1621 gtgcccaacc ggaaagaaga agattgagtt cagcgtggcc gatgagcccg gattgtttgt
     1681 tgagtgtcgt gattccgtta ccgccacgcc ggtttggtac ttgcgcctga agaacgcgca
     1741 gggcaccaac acgtacaaaa agcttggtac cgtcaaagat ctcagccttg cccaagccag
     1801 aaagctggca aagcaattgc gtgccgagca caccatagtg aagcagtcag ttgccgcccc
     1861 cacggcagcg ccgcaggaac tcacgctagc ccgctttttt gacgagcagt atcttcccca
     1921 tgccaaagtg cataaacgct cctatgccaa ggacatcacc ctctaccgtt tgcgtattgc
     1981 cccaaagttt gggcatctcc ccctctcggc catcaaccgc cgcgaggtgc agaagttcca
     2041 caacgatctg ctgtccgaag ggctgagtcc tgcgtcttgc aatcaccatg ttgtgttgtt
     2101 tcgcaggatg ctcaacttgg cctgcagctg ggaactgttg gagaagaacg tcttgcgtgg
     2161 catacctttg ctacccctgg acaatttcag ggaaaatttc ttaagcgcgg aagaaaccaa
     2221 ccgccttgtg gaagtgctga cgacggatgc caacaaaacc gtgtgcgcca ttcttttgtt
     2281 tttgctgaac acgggcgcac gtcgtatgga agccatccag gccaagtggg tggacgttga
     2341 tttggaaaac cgcctttgga agattccggc ggcgaacaac aagtcaaaac gccccaagtc
     2401 actgcccctg aatgacagtg cgatgcaaat cctgcaaggt ctggagtcca agggcaccaa
     2461 tccatacctg tttccgaacc cgcagacagg acttccatac tccggaatca tgcgcgtttg
     2521 gtaccgcttg cgaaagaagg ctggcctcga ccctgacatg cgtgtccacg atctgagggc
     2581 ctgtctggcc gagcgccttt tgagtgccgg acagagcttg tttcttgtgc agaagctgct
     2641 cggacatcaa gacccgcgca cgaccttgcg gtacgcccga ctcagctcga aggcactttt
     2701 ggatgccagc aacatagcta gcgttgcgct gccatctgtg ccaaagcagg ttgcataaat
     2761 gtttaaggag ggcctactgt tcgtggcagg cacctcattt catccagctt tcgcaatgac
     2821 acccgtttcc atgatggtaa ggcgaaatgg tatttgttat tattggatag taattttgga
     2881 gaagactcta tgcctactag cgtcgccctt agtccacatt ttgaatcctt tgtgaagcag
     2941 caggtcagta ctggccgctt taacaatgtc agtgaagtcg tgcgcgcagg tcttcgattg
     3001 ctggaagacc aagagcagct caaatccttg aaggtgcagg aactccggtc cgctgtcgaa
     3061 gcaggcgctg ccagtggtgc cggtgtgaat gccaatgagg tgttcgaccg tttggagcga
     3121 aagtaccgga acgctgctga tgcagcttga actctcgccg ctggcggaaa ccgatctcga
     3181 agcgattggt gactacattg cgatagacag tcagggaaat gccttgcgtt tcattcagga
     3241 attgcgtgac cagtgcagaa aaatcaccaa ggctcccatg gcgtatgttg cccgtcctga
     3301 gttgggtgaa gggctgcgca gctgtgccca tggtcgttat gtgatcttct tcagacccgg
     3361 tgttgaggtt gtccgcgtcg agcgtatctt gcacagtgca ttggatatcc gttcggagtt
     3421 gatgccctag
//
LOCUS       DAOSLL010000531         3422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24125_length_3422_cov_4.934066, whole genome shotgun sequence.
ACCESSION   DAOSLL010000531 DAOSLL010000000
VERSION     DAOSLL010000531.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24125_length_3422_cov_4.934066"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..773)
                     /locus_tag="PLB25_18315"
     CDS             complement(204..773)
                     /locus_tag="PLB25_18315"
                     /inference="COORDINATES: protein motif:HMM:NF015038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HOE43561.1"
                     /translation="MAKHSERFPLYRQAHIFGRAGMELPRSTLAQWVGICGVGLQPMV
                     DALRVEILKHTVLYPDETPVAMLKPGTGKTHRANLWVYAPMAFEDLRAAVCDFCETRA
                     GERARSFPGEWTGSLVCDDYAGYKVGFAGGISEARCMAHSRRKFFYLHVSSKNQIAGQ
                     ACTYISQLYDIEHEIKTPGSDERLNIRLA"
     gene            complement(1033..1299)
                     /locus_tag="PLB25_18320"
     CDS             complement(1033..1299)
                     /locus_tag="PLB25_18320"
                     /inference="COORDINATES: protein motif:HMM:NF024408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOE43562.1"
                     /translation="MCFRPEALIVAIAAKCSDILCRQAKIDKLAHELATLKRWKFGRS
                     SDQLDGPQISLIEEMPEADIATEEQELQELALAAKTSAAPRQQP"
     gene            1354..1728
                     /locus_tag="PLB25_18325"
     CDS             1354..1728
                     /locus_tag="PLB25_18325"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE43563.1"
                     /translation="MSCANWLSDQIHRPSPCQQDKCTSRRVAHPLTEKRQIEIFKIEK
                     TTTARLTVSSEEIGLAQAPVVIAHHLGMSRIAEGVETIAQHKLLLQIGCDFAQGYLYS
                     KPVTAIEVETLFSQHTLLNLPF"
     gene            1839..2306
                     /locus_tag="PLB25_18330"
     CDS             1839..2306
                     /locus_tag="PLB25_18330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOE43564.1"
                     /translation="MKVSDILRIKGGTLYSTTPDDSLVSALALMVDRDIGSLVVIEHG
                     ELVGMLTVRELTQILVKTGGNVVKSTVRAAMDDCPLTCTLETPMNEVRRMMLASHARY
                     MPVMDKNMLLGVISFHDVARAVVDSQSFENKMLKAFIHDWPEGVDTAALPKLS"
     gene            2353..>3422
                     /gene="aroC"
                     /locus_tag="PLB25_18335"
     CDS             2353..>3422
                     /gene="aroC"
                     /locus_tag="PLB25_18335"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HOE43565.1"
                     /translation="MSGNTFGTLFAVTNFGESHGPAIGCVIDGCPPGLALSEADIQPD
                     LDRRRPGTSKFVTQRNEPDAVEILSGVYEGKTTGTPVCLLIRNTDQRSKDYGSIVQTF
                     RPGHADYAYFQKYGIRDPRGGGRSSARLTAPMVAAGAVAKKWLFQKYGTLFRGCMTQI
                     GELQIAFESWQHVPGNPFFAPVADVTALETYMEALRKSGDSCGARLRVSASNVPVGLG
                     EPLFDKMDADIAYAMMGVNAVKGVEIGAGFNSVTQRGSTHGDALSPAGFKGNNAGGTL
                     GGITSGQDLEVSVAIKPTSSILIPRESIDTSGQSTEVITKGRHDPCVGIRATPILEAM
                     LALVVMEHALRHRAQCGDVQAQ"
BASE COUNT          753 a          968 c          958 g          743 t
ORIGIN      
        1 aaaccttgga gccttcacgg taggactcgc gcagcaaaac ggtgggggtg gagccgcgat
       61 ttgggacgat atttatgtgc atgtctacat tatacaacat attaccaatt gaatgaaaaa
      121 ttaatacatg tctacatatt tgcactcaaa ataacaccaa cgtgttgatt tcaaaaggaa
      181 ttcaaccaaa tccggatgga acttcaggct aggcggatgt tcagacgttc atcagagccc
      241 ggcgtcttga tctcatgctc gatgtcataa agttggctga tgtacgtgca cgcctgcccg
      301 gcgatctggt tcttgctgct gacatgcaaa taaaagaact tgcgccttga atgtgccatg
      361 caccgcgcct ctgaaatgcc accggcaaaa ccgaccttgt acccagcgta atcatcacaa
      421 accagactgc ccgtccattc accaggaaat gatctggcac gctcgcccgc tctggtttcg
      481 cagaaatcgc aaactgccgc cctcaaatct tcaaacgcca tcggtgcata aacccacagg
      541 ttagcccggt gggtttttcc tgttcccggt ttgagcatcg ccaccggtgt ctcatccgga
      601 tacaacaccg tgtgcttgag aatctcaact ctgagtgcat cgaccatcgg ctgcagtccc
      661 accccacaga tgcccaccca ttgggccaaa gtcgagcgtg gcagttccat cccggcgcgc
      721 ccgaagatgt gtgcctggcg atacaacggg aaccgttcgc tgtgtttggc caccaatacc
      781 taggccagca agcccgcagt gggagcagcc tcaagtcaat aatctgtgcg ggcacgggtg
      841 cctgagtcag cgtttcgcag tcatcacact cccatttgcg acggatttgc tcttctaccg
      901 tgaacacccc cggtgtgtag tccagttttt ctgagacatc ctgaccaacg cgtttgagtt
      961 gacagccact ggtggtgttg tcgggttcga ggtggatttc taatcggggc aggcccgcgg
     1021 gcagggagtc cgttagggtt gttggcgtgg ggccgcactg gtcttggcag caagtgcaag
     1081 ttcctggagt tcttgttctt cggtggcaat gtcagcctcc ggcatctcct cgatcaagct
     1141 gatctgaggt ccatcgagtt gatcgctgct gcggccgaat ttccagcgct tgagggtggc
     1201 cagttcgtgg gcgagtttgt cgatcttggc ttggcggcac aggatatcgc tgcatttggc
     1261 tgcgatggcc actatcaggg cttcaggcct aaaacacatg caatctggca gccgctgaaa
     1321 gctatgacga agctagctct caatgaccaa ataatgtcgt gtgccaattg gctatcggac
     1381 caaattcatc gcccaagccc ctgccaacag gataaatgta cgtcaaggcg tgttgcccac
     1441 ccacttacgg aaaagcggca aatcgaaatt ttcaagattg agaagaccac cactgcgcga
     1501 ctgactgtca gctccgaaga gatcggtctg gcacaggcgc cggtggtcat tgcgcaccat
     1561 ttgggcatga gtaggatcgc cgagggcgtt gaaaccatcg cgcagcacaa actgctgctg
     1621 cagatcggct gtgattttgc ccagggctat ctgtattcca aaccggtcac tgcaatcgag
     1681 gtagagaccc tgttttcaca gcacacgctg ctgaatttac cattttgacc tgggggtagc
     1741 tggcctgacg ctgtcattcg cacggccaat ccgccggttt tgccattaag atcagcgttc
     1801 aagccgtaaa tcgtcaacca tgtacaacga ggaacaacat gaaagtcagt gatatcttgc
     1861 gcatcaaggg tggcacactc tactcaacca ctccggatga ttctttggtg agcgccctgg
     1921 cgttgatggt cgaccgtgac attggttcgc tggtggtcat cgaacacggc gagctggtcg
     1981 gcatgctgac ggtgcgcgaa ctcacccaga ttctggtcaa gactggcggc aacgtggtca
     2041 agtccaccgt gcgtgccgca atggacgact gccctctgac ctgcaccctt gaaaccccca
     2101 tgaacgaagt gcggcgcatg atgctggcca gccatgcgcg ctacatgccg gtgatggaca
     2161 aaaacatgct gctgggcgtg atcagctttc acgacgtggc gcgtgccgtg gttgatagcc
     2221 agagttttga aaacaagatg ctcaaggcct tcattcatga ttggcccgaa ggggtggaca
     2281 cggcggcact gcccaagctg tcgtgatccg atgccctcgg caccggtgtc tgctctggga
     2341 taatccgccg ccatgagcgg caataccttt ggaaccctat tcgcagtcac caactttggt
     2401 gaatcccacg gcccggccat tggctgcgtg attgatggct gcccgccggg cctggcgtta
     2461 agtgaggctg acatccaacc tgacctggac cgccgtcggc caggcaccag caaatttgtc
     2521 acccaacgca acgaacccga tgcggtagag attttgtcag gtgtctatga gggcaaaacc
     2581 actggcacgc cagtttgcct gctgatcaga aataccgatc aacgcagcaa ggactacggc
     2641 agcatcgtgc agacctttcg ccccggtcat gccgactacg cctattttca aaaatacggt
     2701 attcgtgacc cgcgtggcgg tggtcgctcc agtgcccggc tgacggcacc gatggtggcc
     2761 gccggtgccg tcgccaaaaa gtggctgttt caaaaatatg gcaccttgtt ccggggctgc
     2821 atgacccaaa ttggcgagtt gcagattgcc tttgagtcgt ggcaacatgt gcctggcaac
     2881 ccgttttttg cgccagttgc cgatgtcacc gcgctcgaaa cctatatgga agcactgcgc
     2941 aaaagcgggg actcgtgtgg tgcacggctg cgggtcagcg cctccaacgt accggttggg
     3001 ctcggcgaac ccctgtttga caagatggat gcagacattg cctacgccat gatgggtgtc
     3061 aacgccgtca agggggtcga gatcggagcc ggattcaaca gcgtgaccca gcgcggcagc
     3121 acgcatggcg atgcgctctc accagccggc ttcaagggca acaacgccgg tggcacgcta
     3181 ggcggcatca ccagcggcca ggatctggag gtatcggttg ccatcaagcc gaccagctcg
     3241 attctgatac cgcgcgagtc gattgacacc agcggacaaa gcaccgaggt cattaccaaa
     3301 ggtcgccacg acccctgtgt gggcattcgg gcaacaccga tactcgaggc catgctggcg
     3361 ctggtggtga tggagcacgc gctgcgccat cgcgctcagt gcggtgatgt gcaagcgcag
     3421 tt
//
LOCUS       DAOSLL010000532         3418 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24164_length_3418_cov_4.274160, whole genome shotgun sequence.
ACCESSION   DAOSLL010000532 DAOSLL010000000
VERSION     DAOSLL010000532.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24164_length_3418_cov_4.274160"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="PLB25_18340"
     CDS             <1..216
                     /locus_tag="PLB25_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43566.1"
                     /translation="GDQPAQRSRPAPPPAPGLAAVWFGWNTIQCQSANKASVSELISL
                     LPWIPVERAATLWANPVEVEKANAHKE"
     gene            complement(474..3200)
                     /locus_tag="PLB25_18345"
     CDS             complement(474..3200)
                     /locus_tag="PLB25_18345"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE43567.1"
                     /translation="MKWSINITGKLVAYLLLAGIVPLVVYGVSGFFVARNIVIGQASA
                     YHQRIASDAVAYFELYRSQVEDLAASVVGNEAVIDALFEVDRQAAGSYALLNARAQIG
                     YLLNNFVRVRGLVSIDLFSLKGKHFHVGETLHVNEVDLTTVNRMMQESEAAQGAALWR
                     GIDDNINTASPQKKVITMTRLVQHFSAQTGANQPIGLLVVNLSNEVLHGYFESQAAPL
                     GSRLMATDRHGRLMYHDKPALVGLPMAKALLDLASDPTPTHRLNLDGEEVIVTTMALP
                     NLDGYLFFVAPLALQTDPVNQLALSGVVLLLVCLVGIAGLARYYAKTVVMPIRAVSDG
                     FRQMEAQPEAKHQVLPVPVQQDEIASLIAGFNAHIDSLAAKQVADARLKRLEQAELEN
                     AHILRAAVDAIDASFTVFDESDRLIFYNEQFRQLIRSTDQPEIFTLTFEEILRSGAER
                     GIYLEASGRVDEWVAERMAVHKTDHSDWELKLNDGRWLHIIGRRTPDGQIVSLATDVT
                     SVKQMHEAALAASHAKGVFLANMSHEIRTPINAILGMAHLLQLEGVSSRQAAYFDKID
                     TAAQHLLSIISNILDLSKIEAGKLELEEVPVMPGNLLTNIGAMMSDRARAKGIQIVIN
                     ATYLPQRLVGDAMRLQQALLNYVSNAIKFTQNGTVTLRADVQAETIDAVEVRFEVQDT
                     GIGIAPEAMSRLFNAFEQADNSMTRKYGGTGLGLRITQRLAELMGGAAGASSTPGLGS
                     VFWFTAKLKKGASVANDLQAPADLADVDAKALVQQRYSESRVLVVDDDPVHREVVQRL
                     LKSADLVVDTAADWQEALDLTRQHRYRVIYLSLQMPKVEGSDAVQQIRQIEGYLQTPV
                     IGMTAKVLAQDPLNASAAAVSYCLTKPVDSIALFEVLLSALTELESPQRSVL"
     gene            complement(3197..3364)
                     /locus_tag="PLB25_18350"
     CDS             complement(3197..3364)
                     /locus_tag="PLB25_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43568.1"
                     /translation="MAEVKAGRLAVTVDQQAAEQGFQGVMLATRLIKGDSVPEVTLIE
                     TRLVTANPFKK"
BASE COUNT          771 a         1018 c          868 g          761 t
ORIGIN      
        1 ggcgatcagc ctgctcaacg atcgcgccca gctccgccgc ctgcgccggg cctggcagca
       61 gtttggtttg gctggaacac catccagtgt caatctgcaa acaaggcctc ggtcagcgaa
      121 ctgatttcac ttttaccctg gattccagtt gaacgcgccg ctaccctatg ggcgaacccg
      181 gtcgaagttg aaaaagcgaa tgctcacaag gagtaaccat cccccggcaa agccggaggc
      241 ttcaagaatt gtgagccgct aaaagcgcca taacggggac gcttgcgcgg ccccaactcc
      301 cggtgccact tatgcggtgg cgggtcttga tttccagaat catcggcttg gacgattgcc
      361 acgccatccg cacaaactgc tcaagttgtt ttacaaccaa tcatcaggtc agcgctgttt
      421 tcattcaggt cgaaaaattg gccatgctgt gctcacacca atcgcgcaaa actctaaagc
      481 acagaccgct gcggtgactc cagctcggtc aaggcactta acaacacctc aaaaagtgcg
      541 atggaatcaa ccggttttgt caggcaataa ctcactgcgg ctgcgctggc gttcaacggg
      601 tcttgcgcaa gcaccttggc ggtcatgcca atcaccgggg tctgcaggta accctctatt
      661 tggcgtatct gctgaaccgc atccgaaccc tctactttag gcatctgcaa gctcaaataa
      721 atcacccggt agcgatgctg cctcgtcagg tcaagggctt cttgccaatc cgcagccgta
      781 tccacaacaa gatctgccga tttcaacagc cgctggacaa cttcgcgatg gaccggatca
      841 tcgtccacca ccaatacgcg gctttcactg taacgttgct gcaccagggc cttggcatca
      901 acatctgcca ggtcggccgg ggcttgcaaa tcgttggcaa ctgacgcacc ttttttcaat
      961 ttcgcagtaa accagaaaac actgcccaaa ccaggcgtac tgctggcacc tgctgcacca
     1021 cccatcaact cggcaagccg ctgggtaatc cttagcccga ggcccgtgcc cccatatttg
     1081 cgggtcatcg aattgtcggc ctgctcaaag gcattaaaaa gccgtgacat cgcttcgggg
     1141 gcaatgccaa tgcctgtgtc ttgcacctcg aagcgcactt ccacggcatc gatggtttcg
     1201 gcttgtacat cggctcgcaa tgtcaccgtg ccgttttgtg tgaacttgat ggcattcgaa
     1261 acatagttga gcaaggcctg ctgcaaacgc atggcatcgc ccaccaagcg ctgcggcaaa
     1321 tacgtggcat tgatcacaat ctggatgcct ttggctctgg cacggtcaga catcatggcg
     1381 ccgatattgg tcagcaaatt gcccggcatc accggcactt cctccagttc cagcttgccg
     1441 gcttcgatct tcgacaggtc aaggatgttg ctgatgatgc tcagcaaatg ttgtgcggcg
     1501 gtgtcaatct tgtcgaaata cgctgcttgc cgcgagctca cgccttcaag ttgcagcaga
     1561 tgggccatgc ccagtatggc gttgatgggt gtgcgaattt catggctcat atttgccagg
     1621 aaaacacctt tcgcatgact ggccgccagg gccgcttcgt gcatttgctt cacagaggtc
     1681 acatcagtgg ccaaactgac gatttgtccg tctggcgtcc ttctcccgat gatgtgcagc
     1741 caacggccat cattgagttt taactcccaa tctgagtgat cggtcttgtg caccgccata
     1801 cgttcagcga cccactcgtc cactcgaccc gatgcttcaa gataaatccc ccgttctgcg
     1861 ccggaccgaa ggatctcctc gaaagtcaag gtgaaaatct ctggttgatc tgtagacctt
     1921 atcaattggc gaaactgctc gttgtaaaaa atcagcctat ccgactcatc aaacacggtg
     1981 aaggaagcat caatggcatc aacggcagcg cgcagaatat gggcattttc cagttccgcc
     2041 tgctcaaggc gcttgagtct ggcgtctgcc acttgcttgg ccgcgagcga atcaatgtgg
     2101 gcgttaaacc ctgcaatcaa gctggcaatt tcgtcctgct gcacgggcac tggcaacact
     2161 tggtgctttg cctctggctg ggcctccatt tgccggaaac catcggaaac agctcgaatt
     2221 ggcatcacca ctgttttggc gtaataccgt gccaacccgg caatgccgac aaggcagacc
     2281 agcaaaagaa ccacgccaga cagcgccagt tggttcaccg ggtcagtctg caaagccaga
     2341 ggtgcaacaa aaaaaaggta accatcgagg ttgggcagtg ccatggtggt gacgatcacc
     2401 tcctcgccat caaggttcaa tcggtgcgtt ggcgttggat cacttgccag gtcaagcaac
     2461 gcttttgcca ttggtaaacc aacgagcgcc ggcttatcgt ggtacatcag gcgtccatgt
     2521 cggtctgttg ccatcaacct gctacccaac ggcgcagcct gactctcaaa atagccgtgg
     2581 agcacttcat tgctgagatt gaccaccagc agaccaatgg gctgattggc tccggtctgc
     2641 gcagaaaagt gttgaaccaa acgcgtcatc gtgatcactt ttttttgcgg tgacgcggtg
     2701 ttgatgttgt catcaatgcc gcgccacaac gctgcgcctt gcgctgcctc actctcctgc
     2761 atcattcggt tcacggtcgt caggtcaacc tcattgacgt ggagtgtttc gcccacatga
     2821 aagtgtttac ccttgagcga gaacaggtcg atcgacacca ggcctctgac acgcacaaaa
     2881 ttgttcagca aatacccgat ctgcgcgcgt gcattgagca aggcataact gcccgccgcc
     2941 tgccggtcta cttcaaaaag tgcgtcaatg acagcttcat tgccgacgac actggcagcc
     3001 aggtcctcca cctggctgcg gtaaagctcg aaataagcaa ccgcatctga cgcaattcgc
     3061 tggtggtacg cgctggcttg cccgatgacg atgttgcggg ccacaaaaaa gccgctgacg
     3121 ccgtacacca ctagcggcac gatgccggca agcaacaggt atgcaaccag tttgcccgtg
     3181 atgttgattg accacttcat tttttgaatg ggttggcggt aaccagacgg gtctcgatca
     3241 gcgtcacttc aggcacgctg tcccccttga tcaggcgcgt ggccagcatg acaccctgaa
     3301 atccttgttc tgcagcctgt tgatccaccg tcaccgccaa acgccccgcc ttgacctctg
     3361 ccaccgcctc ggccagcgca tcataggcag ccagcttggc ggtgacggtg gatcaaca
//
LOCUS       DAOSLL010000533         3414 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24196_length_3414_cov_4.448645, whole genome shotgun sequence.
ACCESSION   DAOSLL010000533 DAOSLL010000000
VERSION     DAOSLL010000533.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24196_length_3414_cov_4.448645"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1088)
                     /locus_tag="PLB25_18355"
     CDS             complement(<1..1088)
                     /locus_tag="PLB25_18355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43569.1"
                     /translation="MSTLRIQRIEAKAFKAFKHLDFKIDGRNLLAFGANGSGKSSLYW
                     TLYTFLQSAQKESADVAKYFDKDRPEHLLNLNVTDAERALAAITVTLQNEDASNCPAF
                     TLSLTQHGTQANSDIRKGCLASDFVTYRILFNFYHFRNSQPIDLWPVFESEILPACNT
                     ASVADMLGLWTYLKGADPYGEIQRHSERGSAVKKRYDEFDVLLKKFKTALSEVLATIT
                     IEAQRFYDLHFKHADVLPIKFGVVLRNPDALFDRAAHRFDWPTIGFEVRVGSDTLSRP
                     HTLLNEAKLTQLALSIRFGATFARLDVSPLKLLVLDDLLISLDMGNRMKVVEIILGPT
                     FAGYQKIILTHDSGFFEEFRRSIGGGHA"
     gene            complement(1085..>3414)
                     /locus_tag="PLB25_18360"
     CDS             complement(1085..>3414)
                     /locus_tag="PLB25_18360"
                     /inference="COORDINATES: protein motif:HMM:NF019291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HOE43570.1"
                     /translation="EIALDPELLGNVFENLLASYNPETGTNARRQTGSFYTPRPIVDY
                     MVDESLKAYLADALVKALPTTTAADAKAGLEMLFAYTEKEHAFAEAEIDALITAINAC
                     NILDPACGSGAYPMGILHKLVFILGKLDPHNEKWWQRQVAKAEEIEDVEARASSVQAI
                     NNDFANNALDYGRKLYLIENCIYGADIQPIAIQISKLRFFISLICDQRTHSDKARNLG
                     VRPLPNLETKFVAANTLFGLNLPANLSLFGSPAIGQLEKELERVRHRYFSAQTRVAKR
                     ELQKKDRELVQKIIKLLKTDDFGNEDVYRRIAWNPYDHHSVAEFFDPETMFGPALAEG
                     FDVVIGNPPYVQVKKGIFSKHQFPWSEGKDVGKQNLYKVFLEASYNFLKPNGIASLIV
                     QSSLMADLSSARTREMLLTETKIRYVLEFPKTAPTKAGQVFASVLQGTCICQFQKTAS
                     PDNDFSISCNNDVTTISSLDFETVRQSDLIRFRPGSFEFPLFRPGEFAIYRRLFINAV
                     SLKTLMASITQGDLNLTTNADSFSSKESSVKLYRGRNIHRFSLEETVSDWVKVDCFDE
                     RAKRNGEDTLLVCQEITGTVDPRRLNFTLIDKPGEAFLFGHTVQKIVLKNQAQSKAVL
                     AILNSRLMDWCFRKTSTNNHVGGYELEALPVPTSIPMREVVVLTTLVDYLLAAMRSGE
                     KVVFAFLDTLIDACVLELYFSEHMGERGLGITAYVRALLPDARTILSPDDLVQAAINF
                     YHAANDPKHPIRNILMRIPVDSPDLLALIQREGAT"
BASE COUNT          816 a          825 c          895 g          878 t
ORIGIN      
        1 tcggcgtggc caccgccaat acttcttctg aactcctcga agaatcctga atcgtgggtc
       61 agaataattt tttggtagcc tgcaaaagtg gggccgagaa tgatttcaac cactttcatg
      121 cggttgccca tgtcgaggct gatcaataaa tcatccagga ccaacaactt caagggtgac
      181 acatcaaggc gggcgaaggt tgcaccaaaa cgaattgaca gtgcaagctg tgtcaatttg
      241 gcttcattca atagcgtgtg aggccttgaa agcgtgtcgc tgccaaccct aacttcaaat
      301 cctattgttg gccaatcaaa gcgatgtgct gcacgatcaa ataaggcatc tggatttcgc
      361 aggaccacgc caaacttaat tggcaaaaca tcagcgtgct taaagtgtag gtcgtaaaac
      421 ctctgtgctt ctatggttat ggtggcaagc acttcggaca gagcggtttt gaattttttg
      481 agcaatacat caaattcgtc gtaacgcttt tttacagccg aaccacgttc actgtggcgt
      541 tggatctctc catacggatc ggctcctttg agataagtcc acagtcccaa catgtcggcc
      601 acgcttgctg tgttgcaagc tggcagtatt tcactctcga aaaccggcca gaggtcgata
      661 ggctgggaat ttctgaagtg atagaaattg aaaagtattc gataggtgac gaagtcactt
      721 gctaggcacc ctttgcggat gtcagaattg gcttgtgttc catgctgggt tagcgacaac
      781 gtaaacgccg ggcagttgga cgcatcttca ttttgaagag tcactgtgat cgccgccagg
      841 gctctctcgg catcggtgac attcagattt aacaaatgct ctggccgatc cttgtcaaaa
      901 tacttggcaa catccgccga ctctttctgc gcactttgca ggaaggtgta aagcgtccag
      961 tacagtgacg atttgccaga gccattggca ccgaaggcca atagattccg tccatcgatt
     1021 ttgaaatcta ggtgtttaaa ggctttgaac gccttggcct caatgcgctg aatcctaagt
     1081 gtgctcatgt cgcgccctca cgctgaatca aggctagcaa gtccgggctg tcaacaggga
     1141 tgcgcatgag gatgttgcga atggggtgtt tgggatcgtt ggctgcgtgg tagaagttta
     1201 tggccgcttg gaccaaatca tctgggctca aaattgtcct ggcgtcggga agcagcgcgc
     1261 ggacgtatgc cgtgatgcct agcccccttt cacccatgtg ttctgagaag tacagctcca
     1321 atacacaagc gtcaatcagc gtgtcaagaa aagcaaacac cactttttca ccacttcgca
     1381 ttgccgcgag aaggtagtcc acaagggtag tgagcacaac tacctcccgc attggaatcg
     1441 aagttggaac tggcagggct tcgagttcat agccgccaac atggttgttg gttgaagttt
     1501 ttcggaaaca ccagtccatc agccgcgagt tcagtatcgc caagactgct ttcgattgcg
     1561 cctgattttt cagcacaatc ttctgtacgg tatggccgaa caaaaatgct tctcctggtt
     1621 tatcaatcaa ggtgaagttc agtcgccgag gatcaaccgt tccggtaatt tcctgacaga
     1681 ctaacagcgt gtcttcgcca tttcgttttg cccgttcatc aaaacaatct actttcaccc
     1741 aatcggagac ggtttcttca agactgaatc gatgaatatt cctaccgcga tacagtttta
     1801 ctgacgattc tttgctagaa aatgaatcgg cgtttgttgt taaatttaaa tcgccttgcg
     1861 taatgcttgc catcagagtc ttgagcgaga cagcattaat gaaaaggcgg cgatagatgg
     1921 caaattcccc aggtctgaag agcgggaact cgaagctacc ggggcgaaag cgaatcaggt
     1981 cgctttgtcg gacggtctcg aagtcaagcg atgaaatcgt ggtgacatcg ttgttgcatg
     2041 aaatagaaaa atcattgtca ggggaagcgg ttttctgaaa ttggcagatg caagtgcctt
     2101 gaagtacgct cgcaaaaacc tgcccggcct tcgtcggcgc agtttttggg aactccaaaa
     2161 catatctaat tttggtttcg gttagaagca tttcgcgcgt tcgggctgaa ctcagatctg
     2221 ccatgagcga gctttgcacg atcaagctgg cgatgccatt cggcttcaag aagttgtatg
     2281 aggcctctag aaaaactttg tacaggtttt gcttaccaac atccttaccc tcagaccaag
     2341 gaaactgatg cttagagaaa attcctttct tgacctgtac gtagggcggg ttgccaatca
     2401 ccacgtcaaa gccttccgcc aaagcggggc caaacatggt ttcggggtca aaaaattcgg
     2461 ccacgctgtg gtggtcgtat gggttccagg cgatgcggcg gtacacgtct tcattgccaa
     2521 aatcatcggt cttaagcagc ttgatgatct tctgcaccaa ttcgcggtct tttttctgca
     2581 gctcgcgttt tgccacgcgc gtctgggccg aaaagtagcg gtggcgcacg cgttcgagtt
     2641 ctttttcaag ttgcccaatg gcggggctac caaacaggct cagattggct ggcaagttta
     2701 agccgaacag ggtattcgcg gcgacaaact tagtttccaa attaggcagc ggcctcacgc
     2761 ctagattgcg cgctttgtcg ctgtgggtgc gttggtcgca aatcaaggag ataaaaaaac
     2821 gcagctttga aatctggatg gcaatgggct ggatgtctgc accgtagatg cagttctcaa
     2881 tcaggtacag cttgcggcca tagtccagcg cgttgtttgc aaagtcgttg ttgatggctt
     2941 gcaccgatga agcgcgggct tcaacgtctt caatttcttc ggccttggct acctggcgct
     3001 gccaccattt ttcattgtgc gggtccagct tgcccaaaat aaacaccagc ttgtgcaaga
     3061 tacccatggg gtaggcaccc gacccacatg ctgggtctag gatgttgcag gcattgatgg
     3121 ccgtaatcag cgcgtcaatc tctgcctctg caaaagcgtg ttctttctcg gtataggcga
     3181 acagcatttc cagtccggct ttggcatcag ccgccgtggt ggtgggcaaa gctttgacca
     3241 gagcgtctgc aagataggcc ttgagggatt cgtccaccat gtaatccacg atggggcgcg
     3301 gcgtgtaaaa cgagccggtc tggcggcggg cgttggtgcc ggtttcgggg ttgtagctgg
     3361 ccagcaggtt ttcaaacaca ttgcccagca gttcgggatc gagggcgatt tctt
//
LOCUS       DAOSLL010000534         3410 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24230_length_3410_cov_4.408346, whole genome shotgun sequence.
ACCESSION   DAOSLL010000534 DAOSLL010000000
VERSION     DAOSLL010000534.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24230_length_3410_cov_4.408346"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            139..273
                     /locus_tag="PLB25_18365"
     CDS             139..273
                     /locus_tag="PLB25_18365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43571.1"
                     /translation="MRNPFATATAIGSANGVHSMAAALVREIGLQRLWNMRKQLSIQD
                     "
     gene            393..657
                     /locus_tag="PLB25_18370"
                     /pseudo
     CDS             393..657
                     /locus_tag="PLB25_18370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(862..1089)
                     /locus_tag="PLB25_18375"
     CDS             complement(862..1089)
                     /locus_tag="PLB25_18375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43572.1"
                     /translation="MMGVLDRMPLKWLAAVALWLAVAPIMPEPHLIEKLRMLSQGTLR
                     QPLDIFDLLLHSVPMALLALRVWRDARRRQC"
     gene            complement(1086..1346)
                     /locus_tag="PLB25_18380"
     CDS             complement(1086..1346)
                     /locus_tag="PLB25_18380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018903996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich CWC family protein"
                     /protein_id="HOE43573.1"
                     /translation="MNTAPDPTRCPLCGKENRCAMEIERASGEPQPPCWCSTQRFPAD
                     LLARLPSEAAGKACICANCLSGFLNPRPAPASTPASAPVNAA"
     gene            1527..1670
                     /locus_tag="PLB25_18385"
     CDS             1527..1670
                     /locus_tag="PLB25_18385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43574.1"
                     /translation="MGRPTAYADWGAHLAQRTAVSGPRRSLHQFERRVKRWAGLPLRW
                     VAT"
     gene            complement(1915..2550)
                     /locus_tag="PLB25_18390"
     CDS             complement(1915..2550)
                     /locus_tag="PLB25_18390"
                     /inference="COORDINATES: protein motif:HMM:NF024852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HOE43575.1"
                     /translation="MIKTISIIGASAGIGLQCVQVALQRGHRVISLSRKVDTLPDHPN
                     LQVIQGSATKTDDLRRALNGADAVLVALGTGVDRKPTTLYTDFAKALLPLQSELGQTP
                     IQLLTGFGTGDSAQYQGLVARWLFRLLLKAVYENKTAMEQMIESSTLNWSLVRPGMLT
                     NASTVAPARVQVDYTPGMKVGAVSRMAVARFMVEQAEHPTCQKRKPALSAR"
     gene            3184..3410
                     /locus_tag="PLB25_18395"
                     /pseudo
     CDS             3184..3410
                     /locus_tag="PLB25_18395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429967.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter periplasmic adaptor subunit"
BASE COUNT          645 a         1035 c         1051 g          679 t
ORIGIN      
        1 gcagcgctgc gtctggcatg cccgttgcag cggaaattgc gatcaatgac agttcctttg
       61 gcgccaaacc tgtcacgcaa ggcagaaagt cgaggtccag cacgccggtg ttggccagtg
      121 cggtagagga aacagccatt gcgaaatcct tttgcaactg ccaccgctat cggttcggcc
      181 aatggcgttc attcgatggc cgccgcgctt gtaagggaaa tcgggctgca gcgcttgtgg
      241 aatatgcgca agcagctatc aattcaggat tgattgacgt gcctgctggc cgcaccacaa
      301 atacatgttg ctgtatagag ccacagcatc attggtcacc cagcttctgc tggcactgcc
      361 catgcccgcc accgcccacc caacgcaagc acattcgcgc gcgtattcgc aagcgggatg
      421 caaagcccaa gcgctacaaa accgctgccg agcacttcaa gacctgccat gaactcgcta
      481 gcgcgggcca gttctaccgg cagggcgacc accacacccc aaggctgatg cccgccaggc
      541 gcttcgcaaa tacctcgaat gcggcatctt tgcgtatggc tttgccaaag cttgggtggt
      601 gacgacagcg cgcaggacta cttcgtcgcc tattcctgca aagggctggg catctgcgca
      661 agcgtgcgca gcttggtttc gttccttttg atcactatcg ctggatgatc aacctgttga
      721 ccatttggtg agttgccgac cccgctttga gctcactgca gggtggaaat acgctagccg
      781 ttcaggctca cacctcgttg gacaaggctg tggtgctggc ctttgtggag agctaaggct
      841 cgcgccgagt ctggcaggca cctagcactg gcgccgccgc gcgtcgcgcc acacgcgcaa
      901 cgccagcagt gccatcggaa cgctgtgcag caacaggtcg aagatgtcca gcggttggcg
      961 caacgtgcct tggctgagca tgcgcagctt ttcgatcagg tggggctcgg gcatgatcgg
     1021 tgccaccgcc aaccacagcg caaccgccgc cagccacttc aacggcatcc gatccagcac
     1081 acccatcatg cggcgttcac tggggcgctt gcaggggtgc tcgctggggc ggggcgggga
     1141 ttgagaaaac cgctcagaca gttggcgcag atgcaggcct tgcccgccgc ttcgctcggc
     1201 agccgcgcca gcaagtcggc gggaaagcgc tgcgtgctgc accagcaggg cggctgaggc
     1261 tcaccgctgg cgcgctcgat ttccatcgcg caacggttct ctttgccaca tagcgggcaa
     1321 cgggttgggt cgggggcggt gttcatggtg gcagtgtata cggacgcccc aacacgggcg
     1381 ctgagcccgt cgccgctcgg cagtgaagtg cgccaccagc ggccttgccc caggcccggt
     1441 acaaggcgcg cccaacgacg cgcagcgcat ggcgctactg gatgatattc tggagccgcg
     1501 ttggtcggct tgtcgcggcc ccgcgcatgg gcagacccac cgcctacgcc gactggggcg
     1561 ctcacctggc acagcgcacg gcggtgtcgg ggccgcgccg cagcctgcac cagttcgagc
     1621 gccgggtcaa acgctgggcc gggctgccgc tgcgctgggt ggcaacctga tccagcgcga
     1681 cccgcagcaa atgctcctga gaacagttgc gttgccaatc cacaccaaac tccgcgccct
     1741 tgagaagcag ctctgacagc aaaggtgaga tctgcaccac gtggcaagca agcccgccag
     1801 ctttggccat cgtcgcattc acatacagcg tgcaaaagtc aaagcgcctc ctggaaagca
     1861 ccgcatgctg ctggcctggc ggtatccaca caccgcgctg cggtggcttg tctgctaccg
     1921 cgccgacagg gcaggtttgc gcttctggca agtggggtgc tctgcctgtt cgaccatgaa
     1981 acgggccacc gccatgcggc tgacggcccc gaccttcatg cccggcgtgt agtccacctg
     2041 cacccgtgcc ggcgcaactg tgctggcatt ggtcagcatg cccgggcgta ccaatgacca
     2101 gttgagtgtg ctggactcga tcatttgctc catcgctgtc ttgttttcat agacggcttt
     2161 gagcagcagt cgaaacaacc acctggccac cagtccctga tactgcgcgc tgtcacccgt
     2221 gccaaagccg gtgagcaatt gaatgggtgt ctgccccagt tcagactgca acggcagcag
     2281 tgctttcgca aagtcggtgt agagcgttgt gggcttgcgg tccacgccgg tgcccaaggc
     2341 gacaagcact gcgtcagcac cgttcagagc gcggcgcaaa tcgtctgtct tggtggcgct
     2401 tccttgaatg acctgcaagt tcgggtggtc gggcaaggta tctaccttgc gcgagaggct
     2461 gatgacgcgg tgcccgcgct gcagcgcgac ttgcacgcac tgcaagccaa tacctgcaga
     2521 tgcgccaatg atgctgatcg tttttatcat ggcagaagtc cctcttctat ctggttggtt
     2581 ggtaataatg tgccgttggc ccatgcatgg gataccttca gcaggccttt tgcccaatgc
     2641 aacgcttcgt tgttgcgcgg ggcaaagata aacaccgagc cctgcgggcc agggatcgga
     2701 aaggctcggg caacatggcc tgcatgctgc cggtcggcgg attgtggatg tgggccaact
     2761 cctcgcgcgc caagaatgcc attggatcgc gattgagaac ctcgggcaag gtcagtgccc
     2821 acgatccatc tggcgcaacg atggtcggtt gcgcaacaca ccctggcaag gagaaggcgt
     2881 catctgcaag tgcctcaatc aggcaccgcc gcgctggcca ctggtccagt tgctgttgcg
     2941 gatttgcagg ggtcatgcgt ggcgcggagc ctgcgcgtgg ggccagggaa gtcagctttt
     3001 gccaatcaat ggagatcgtc atcagtgtgt tgtttgggta aggtttgatt catacacttc
     3061 attgcacatt gttcgtggca gacatctgat gctgaatgtt atccgtgcaa tttcaatttc
     3121 tcacgcatgg ttacggtcgt gggtggatcg tcatccggcg cttcggcggc ggtgcgactg
     3181 aaacaaaccc gcaatgcacc ggtctatgtg aatttgcccg cggcagcgct cgctgccaag
     3241 gcgctgtccg atgctttcaa acccggcatg tttgcccgag gcgattttta gctgggacaa
     3301 tcaagcgcac tcaccgtggt gcaaagtgcc gtgctggtgc gcgacggctt tagcgatgtg
     3361 atgcgggttg gcgcagacaa tcgggtcagc cagctcaagg tggtcaccgg
//
LOCUS       DAOSLL010000535         3408 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24251_length_3408_cov_6.315538, whole genome shotgun sequence.
ACCESSION   DAOSLL010000535 DAOSLL010000000
VERSION     DAOSLL010000535.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24251_length_3408_cov_6.315538"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            74..295
                     /locus_tag="PLB25_18400"
     CDS             74..295
                     /locus_tag="PLB25_18400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3079 domain-containing protein"
                     /protein_id="HOE43576.1"
                     /translation="MAKKFPIHPTRPERICWGCDQLCAADAMACANERLPHPAELFGD
                     DWLTWGEQQLAPVATSVAATVISKTQPDH"
     gene            296..1489
                     /locus_tag="PLB25_18405"
     CDS             296..1489
                     /locus_tag="PLB25_18405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HOE43577.1"
                     /translation="MSLPIFGQSPVSSLPHGLPWLRLAFRPFYLGAALFAALAIPLWL
                     AVILGAITWLPTPPPPLWHAHEMLFGFAIAVIVGFLMTAGKSWTGLATPRGPALGLLF
                     LLWLGARIAALGAPYAMFAVLDLALLPAVGLIFLRLLLRARNWRNLPLAFILLLLSLV
                     NLSFHLSVNGMIDLAPMRSLYAGLAFIVMIECVIAGRVVPFFTTSAIVGLKIVALPWL
                     ERSALAATAVGLVLWVTDWHTGLAAAALALACALHLGRQWRWHPLATRSRPILWILHL
                     SHLWLALGLGLLALAQMGLVPVSIGVHPLGVGATGGLIMGMMTRTARGHTGRALTADA
                     PEIAAYALVMLAALLRVLLPLLAPSLTLYSLICAAAAWSVAFLIYLWQYTPWLVSARP
                     DGKDG"
     gene            complement(2847..3179)
                     /locus_tag="PLB25_18410"
     CDS             complement(2847..3179)
                     /locus_tag="PLB25_18410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43578.1"
                     /translation="MLAIALPRLSWHTTNSSQATGRTFVHEMPAKTFCCQRPLGARHA
                     CRRSWPLFSGLAALQNPACLWIGGPEDGRLQDMQTAPKSGPELDLVIDQDVPPVQLRY
                     LNSPAASL"
BASE COUNT          596 a          981 c         1032 g          799 t
ORIGIN      
        1 cgcccaaatc cgccggtgca aataggggaa gttatttctg accacatcct aagcctgaaa
       61 aacccctgtc ctcatggcca aaaaattccc gattcatccc accaggcccg aacgcatttg
      121 ctggggctgc gaccaattgt gtgccgccga tgccatggcc tgtgccaacg agcgcttgcc
      181 ccacccggcg gagctgtttg gtgacgactg gctgacttgg ggtgagcagc aactggcacc
      241 ggtggcgaca tcggtggccg caaccgtaat ctcaaaaacc caaccggatc attgaatgtc
      301 attacccatc tttggccaat cacctgtatc gtcgctgccg cacggcttgc cgtggttgcg
      361 gctggcgttt cgcccgttct atttgggtgc ggcccttttt gctgccttgg caattccgct
      421 gtggctggca gttattctgg gtgccatcac ctggttgccg acgccgccac cgccgctttg
      481 gcacgcgcat gagatgttgt ttggatttgc catcgccgtc attgtgggat ttctgatgac
      541 ggctggcaaa agctggaccg gcttggccac cccgcgaggc cccgcgctgg gcctgctgtt
      601 tttgctctgg cttggcgcac gcatagccgc cctgggggcg ccctatgcaa tgtttgcggt
      661 gcttgatctg gccttgcttc ccgcggtggg gctgatcttt ctgcgcctgc tgctacgcgc
      721 ccgcaactgg cgcaacctgc cgctggcgtt cattttgctg ttgctctcgc tggtgaatct
      781 gagctttcat ttgtcggtca atgggatgat cgacctggcc ccaatgcgca gtttgtatgc
      841 aggcctggct ttcatcgtga tgatcgagtg cgtgattgcc gggcgcgttg tgcccttttt
      901 caccaccagt gccattgtcg gcctgaaaat tgttgccctg ccctggctgg agcgcagtgc
      961 gctggcggca actgccgtcg gcctggtgtt atgggtgacc gactggcaca ctggacttgc
     1021 cgccgcagcg ctggccctgg cctgtgcgtt gcacctgggt cgtcaatggc gctggcatcc
     1081 gctggccacc cgcagccgcc ccattttgtg gatattgcac ctgtcgcatt tgtggctggc
     1141 gctgggatta gggctgctgg ccctggcaca gatgggcttg gtgccggtgt cgatcggggt
     1201 ccatccgctg ggggtggggg ccaccggtgg cttgatcatg ggcatgatga cgcgcaccgc
     1261 gcgcggccat accgggcggg ctctgacagc agatgcgcct gagatagcgg cttacgcatt
     1321 ggtcatgctg gcagcactgc tgcgggtttt gctgccgctg ctggcaccat cgctcaccct
     1381 ctattcactg atttgcgcag cggcggcctg gagtgtcgct ttcctgatct acctgtggca
     1441 gtacacaccc tggctggtga gtgcgcgccc ggatggcaaa gacggttgat ccaggattcc
     1501 tcggttgaga ccgcctgttg tcatcttcaa atcatcctga gcatttttct cgatgaggtt
     1561 tgccaactcg gtaacagcgc tacgtacccg attgaaccgc tggcaacgcg actgggggcg
     1621 acgcgcctgc ctcgtccgcc aggaagacat gctcttcagt tgctgagcat cgtcgtgcct
     1681 cgccgctcgc tgcgacatgc agggcagggc gcagcgatgc caaaagactg gtctgctgtt
     1741 ggccgtccaa acctgcgcag gcacctttgg cgccgtggcg ttcgccctgt ccggccgcgc
     1801 caccaacggc ccactcgggc gcgcgcaacg gaggtagctg ggttgaatgc gccttttcaa
     1861 caacccgatg cttgctaaaa cgcgccgacg ctagcatgcg aggccccaag gccggttaca
     1921 ggcagcgcca tgcgggttgc tgtttggatt gggatcggac cccgattgac cctgcgctgg
     1981 ctggcacggg ctgggactct cacgatgctg tggttgtgcc caatttcaag atgtctggac
     2041 cctggctttc cagggtttga atagctatct aaataatagc tgctagcgca tattccacaa
     2101 gggctagagg ctgatttgac ccatatatcc tgatttgcat gcagcaggcc gacgcagtga
     2161 gctttatgcc aggtctgggt tggctgcatg aatcgcccgt cggctctggc cggtgttgcg
     2221 cgcccacccg gcaattgcgt tggtgggcag cagcttggcg aattattttg aggtggcctg
     2281 accctggcat cggtcatggc gttttctgct gtttgttggg gttgaaagaa ggtggtcaat
     2341 gtcatcgcgc ggggtggctg caagtcgctt catccggact tgaaccggcc cgacaccgca
     2401 agccaccttg cgggccagac tttgctgaag caggcaaagc ggcggcggga gtgctgccga
     2461 ctgcttgaat gctaaatgcc caagccggac gagccggaat tggacggcct tcgtcgcgag
     2521 ggcgcgactc ctgcacaaac agtcggcgcc catttgaatt tgtaggagtc gcgccctcgc
     2581 ggcgaatgac tttgaatgtt tgaattcttc gcatgatatg caaagacttg acttgttaga
     2641 gactaggcat tttcccagac aagctggaaa aatgaggttc gcctttggcg aacagccatg
     2701 gcccacaaca ggttcaaaac gggatgcctg ctgcccgaga ctgtcggcaa agaccgacaa
     2761 catgttggta agtttgacgc gcatagcgcc aaattggcct ctagcccttt actcatgtgc
     2821 gctgccagct acattttaca tagcaatcaa agtgatgctg ctgggctgtt caggtagcgc
     2881 aactgcaccg gcggcacgtc ttgatcaatc accagatcaa gttcagggcc cgatttcggt
     2941 gcggtttgca tatcttgcaa gcggccatct tccgggccgc caatccacag gcaggccggg
     3001 ttttgcaatg ccgccagtcc ggaaaataag ggccaggatc gacggcacgc atgtcgcgcg
     3061 cccagcgggc gttggcagca aaaagttttc gcaggcattt cgtgtacgaa agttcttccg
     3121 gttgcttggc tcgaattggt cgtatgccaa cttaaacgag gcagcgcaat ggcaagcacg
     3181 ctgaccatca tgcgcaaatt cgccttcttg ttgctgcagg gcttggggtt tggcgtttgc
     3241 ggcctatcat tgaattgaca gcgaaaatgc attcatgatg attcaagatt cccaccaggg
     3301 caccggtttg ctccttaccg attcggcgcg gttgctgcgc aaactgatgg atcggcgatg
     3361 gcagtggctt gacttgtcgc gcgcgcactg gtcaatgctg gcgaccct
//
LOCUS       DAOSLL010000536         3405 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24279_length_3405_cov_5.006567, whole genome shotgun sequence.
ACCESSION   DAOSLL010000536 DAOSLL010000000
VERSION     DAOSLL010000536.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24279_length_3405_cov_5.006567"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="PLB25_18415"
     CDS             <1..658
                     /locus_tag="PLB25_18415"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE43579.1"
                     /translation="GVYQRAGDVDAALKELTSAVTKHPESWQAHALLARLFVARGESK
                     QAIGASKKAIDLKPENASIHNLLARAYEETGEFDLALKAFNKAAELDPAMVTALLGVA
                     RMYIEQGNLVEAKRGLADLSKGSRSLMLVHRMLAEILMREGEYDDAVAEFKAAILHGE
                     KLAENHPEVLTIVPSPGDSRKTAQAYQKVFAKIASSDNHAAKTRREHASDDSTGYSLL
                     "
     gene            complement(943..1488)
                     /locus_tag="PLB25_18420"
     CDS             complement(943..1488)
                     /locus_tag="PLB25_18420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HOE43580.1"
                     /translation="MIEHQHPPKLDVRHAAQSSAHLSGHDLLANYERLMHETQGLGSE
                     NTLNWSAQAGLRADQAGLACDWLHLTVATTLPLVCQRCLGQVDVLVQVAQDFRFVASE
                     GIAEQQDDEAEEDLLVTSRDFDLAGLIEDEVLLALPLVPRHDTCPVAVMMAVADADFQ
                     EAPEKPNPFAQLAQLKGQTPG"
     gene            1515..2114
                     /locus_tag="PLB25_18425"
     CDS             1515..2114
                     /locus_tag="PLB25_18425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HOE43581.1"
                     /translation="MTHTPPRQLILASTSVYRQQLLARLRLPFTVLASQVDEMPLGHE
                     SPQQMACRLALAKAQAVAALHPEAVVIGSDQVADLDGVPLGKPGHHARAVAQLQTMRG
                     KTVVFQTAVAVVCLATRFAQTDLAQVKVKFRNLSNAQIEAYLLAEVPYDCAGSAKSEG
                     LGIALLERIDSDDPTALIGLPLMRTCRLLEAAGLQVLGT"
     gene            2354..3130
                     /locus_tag="PLB25_18430"
     CDS             2354..3130
                     /locus_tag="PLB25_18430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOE43582.1"
                     /translation="MNTPGNPPKGTLYLVPAPLDFGCNETSDLQATLPLGTLQVAASL
                     THWICENAKSTRAYLKRIDAITPLCQNIQQMSLVELPREVHKKGDHLGNFDALPLLQP
                     ALVGHHLGLVSEAGMPAVADPGSSVVRAAHQAGIAVVPLVGPVSLLLALAASGLNGQN
                     FAFIGYLPVDAVERVQRIRDLESLALKSGQTQLFIETPYRNLVMLQALLQTLQHNTRL
                     TLSSGLTLARGQTASDSVKNWKHKTVPLDNATPAVFAIGR"
     gene            complement(3302..>3405)
                     /locus_tag="PLB25_18435"
     CDS             complement(3302..>3405)
                     /locus_tag="PLB25_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOE43583.1"
                     /translation="TRRDNVAERLIKRFGAAFGDGAVRALRSSAVLH"
BASE COUNT          716 a         1035 c          986 g          668 t
ORIGIN      
        1 tggcgtctat cagcgcgcgg gcgatgttga tgctgcgctg aaagagctta cctctgcagt
       61 gaccaaacac ccagaatcct ggcaggcgca tgcgttgctc gccagactat ttgtcgctcg
      121 tggtgaatcc aagcaggcca tcggtgcaag caagaaggcg attgacttga agccggaaaa
      181 cgcatcgatc cacaacctgc tggcgcgcgc ttatgaggag accggcgaat ttgatttggc
      241 ccttaaggca ttcaacaagg cagccgagct tgaccccgcg atggtgacgg cactgcttgg
      301 cgttgcacgg atgtacatcg aacagggcaa cttggtggaa gcaaaaaggg gattggccga
      361 cttgagcaaa gggtcccgca gtctcatgtt ggtccatcga atgcttgccg agattctgat
      421 gcgcgagggc gagtacgacg atgccgttgc cgaattcaag gcggccatcc tccatggtga
      481 aaaattggcc gagaatcatc cggaagtact gaccatcgtt cccagtccag gcgatagccg
      541 gaaaacggcc caagcctatc aaaaggtttt cgcgaagatt gcatccagtg acaaccacgc
      601 ggctaaaacc cgcagagagc acgcttcaga cgattccaca gggtattctt tgctttgaat
      661 ctggtctcca agaggttcag tctgcacatt tcttgcgaag aaatctgatg tgtcaagatc
      721 tggacagtgg caagttaccc cttgagttca agccgccatt ggctgtgcag cacaaggcgg
      781 gtcatatcgg cggtatttgg cgatgatttc cgccgggtca ggagccaaat agcgaattgc
      841 caaccgttgc ggtagggcaa tgcgcagggg caagttgggc cgctgcagtt gtgtcgcagc
      901 cgaggcacca ttccaccggc aggccccggt attgaccctg ggtcagcctg gtgtctggcc
      961 cttgagctgg gccagttggg caaacggatt gggcttctcc ggtgcttcct gaaagtctgc
     1021 atccgccacc gccatcatga cggccaccgg gcaagtgtcg tgacgtggca ccagcggcaa
     1081 agccagcagc acctcgtctt cgatcaggcc agccagatca aagtcccgac tggtaaccaa
     1141 caggtcttct tcggcttcat catcctgctg ctcggctatg ccttcactcg ccacaaagcg
     1201 gaaatcctgg gccacctgca ccagcacgtc aacctggcca aggcagcgct ggcagaccag
     1261 cggcaaagtg gtcgccaccg tcaaatgcag ccagtcgcat gccaaccccg cctgatccgc
     1321 ccgcagccct gcctgtgccg accagttcaa ggtgttttcg ctccccagcc cctgtgtctc
     1381 atgcatcaga cgctcgtaat ttgctagcaa atcgtgtccg ctaagatgcg ctgaagactg
     1441 cgctgcgtgt ctgacatcga gttttggtgg gtgctggtgt tcaatcatgg ggatcagtgt
     1501 aagagaatcg ggacatgact catacgccac cccgtcagtt gatccttgcc tccacctcgg
     1561 tttaccgcca gcaactgctg gcgcgacttc gcctgccctt taccgtgctc gcctcacagg
     1621 tcgatgagat gccgctgggc catgaaagcc cgcaacagat ggcttgtcgg ctggcgctgg
     1681 ccaaggcgca ggcggttgcc gcgctgcacc ccgaggctgt ggtgattggg tcggatcagg
     1741 tggcagacct tgacggcgtg ccactgggca aacccggcca ccacgcacgc gctgttgcac
     1801 agttgcaaac catgcgtggc aaaaccgtgg tgtttcagac ggcggtggcg gtggtttgcc
     1861 tggcaaccag atttgcccag accgatctgg cgcaagtcaa ggtcaagttc cgcaacctca
     1921 gcaatgccca gatcgaggcc tatttgctgg ccgaagtgcc ctacgactgc gctggcagtg
     1981 ccaaaagcga gggattgggc attgcgctgc tggagcgcat tgacagcgac gacccaaccg
     2041 cgctgatcgg cctgccgctg atgcgcacct gccgcctgct cgaagccgcc ggactgcagg
     2101 tgctgggcac atgatcctgg attcctcagt tgaccgcttg ttttcatctg taagtcctta
     2161 tgagcattta cttcttcagc ttcgatgggg ttcacccatt gggtgacagc gctacgcacc
     2221 cgattgaacc gctggcaacg cgcctggggg cgttgctcct cctagcgggc aggagctgtg
     2281 cgtcgtcgtc gcgcctaccc agacgggctg ccagcggccc actcgggcgc gtccaactga
     2341 ggaatctagg atgatgaaca cgcccggcaa tccgcccaag ggaacgcttt acctggtgcc
     2401 cgcgccgctg gatttcggct gcaatgaaac gtcagacctg caagccacgc tgccgctggg
     2461 cacgctgcag gtggcggcca gcctgacgca ctggatttgc gagaatgcca agtccacccg
     2521 tgcctacctt aagcgcatcg acgccatcac gccactgtgc cagaacatcc agcagatgtc
     2581 tctggtcgaa ctgccacgcg aggtccataa aaagggtgac caccttggca attttgatgc
     2641 cctgccactg ctgcagccgg cgctggtcgg tcatcacctg ggtctggtca gcgaagccgg
     2701 catgccagcc gtggccgacc ccggctcgtc ggtggtgcgg gcggcgcacc aggcgggcat
     2761 cgcggtggtg ccgctggtcg gcccggtctc gctgctgctg gccctggcag ccagtggctt
     2821 gaatggccag aactttgcct ttatcggcta tttgccggta gatgccgtcg agcgggtgca
     2881 gcgcatccgt gatctggagt ctctggctct caaaagtggt cagactcagc ttttcatcga
     2941 aacaccctat cgcaacctgg tgatgcttca ggcgctgctg caaaccctgc aacacaacac
     3001 ccgactgacc ttgagcagcg ggctaaccct ggcacgcgga caaaccgcca gcgacagtgt
     3061 gaaaaactgg aagcacaaaa ccgtgccgct ggacaacgcc acgccggcgg tgtttgccat
     3121 tggccgctaa ccaaaggcca tgcaacggcc aatccagacg cctgcaagcg atcctggaac
     3181 aatcaaaaaa gatagctgcc agcgcttgtg cagtaaaggc tagaagccaa aaagaccaaa
     3241 aaacctcaac tttaagccga accaggttcc catgctggct tgcgcaattt cacagcagcc
     3301 atcaatgcag cacagccgat gagcgcagcg cccgtactgc accatcccca aatgctgcac
     3361 caaagcgctt gatcagacgc tccgccacat tgtcacggcg ggtgt
//
LOCUS       DAOSLL010000537         3404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24286_length_3404_cov_3.385488, whole genome shotgun sequence.
ACCESSION   DAOSLL010000537 DAOSLL010000000
VERSION     DAOSLL010000537.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24286_length_3404_cov_3.385488"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /gene="dcuC"
                     /locus_tag="PLB25_18440"
     CDS             complement(<1..857)
                     /gene="dcuC"
                     /locus_tag="PLB25_18440"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DcuC"
                     /protein_id="HOE43584.1"
                     /translation="MTFWAGYLIIKKFKPQPVLFMAGLALMFLAVLLGLGTILPAKES
                     TGLVLFDAFEFIKKTFASRAGGLGLNIMAVGGFARYMDHIGASKALVKLTIRPLVALR
                     SPYLVMAGVWIMGMCLGLAINSASGLAMLLMVTAFPVLISLGVSRLSAVAVIATTLCL
                     DWSPSDTGTILAATTAGIDPVKYWVKYQVPVALAVIPVVAVLHFFTQKWMDKRDGHVV
                     QVTGLIAPKGKDGEVEKIPPMYYAILPIIPLALILIFSDLWISWIKMDIIKAMLIGLA
                     LAMITEFVR"
     gene            1348..2517
                     /locus_tag="PLB25_18445"
     CDS             1348..2517
                     /locus_tag="PLB25_18445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HOE43585.1"
                     /translation="MHQGKLVFAQLMLYLPLSTFRRCVADHNGEHKVKDFSCLDQFFA
                     MAFAQLTYRESLRDIEVNLRAQGRRLYHMGFRCQTISRNTLANANATRPWQIYADFAK
                     HLIGLARPLYATEPFGVELDAAVYAFDASTIDLCLSVFAWAPFRSTKAAIKLHTLLDL
                     RGNIPSFIHITDGKTHEVNVMDSLLLEPGAFYLMDRGYLDFARLFVIHEAQAFFVTRS
                     KSNTKFKRRYSHPVDRIKTKVLCDQTGVLTTYLSSKDYPTTMRRVVIRDDTGKRLVFL
                     TNNFALKPELIADLYRQRWQVELFFKWIKQHLRIKVFFGTGENAVKTQIWIAISTYLL
                     IAIAKKRLHLDHHSLYEILQILSLSMFENIPINQLLKPPSTNSDADFESNQLALL"
     gene            complement(2532..2834)
                     /locus_tag="PLB25_18450"
     CDS             complement(2532..2834)
                     /locus_tag="PLB25_18450"
                     /inference="COORDINATES: protein motif:HMM:NF012892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase C-terminal
                     domain-containing protein"
                     /protein_id="HOE43586.1"
                     /translation="MFQYALARDGQSHALTLAFTTFVLFQFFNVFNARSEHSTVFNTN
                     FFSNVKLWLALGAVLGMQVLAVHWGPAQTIFDTVDLAWGNWLLAIAVASSVLVLDQ"
     gene            3058..>3404
                     /locus_tag="PLB25_18455"
     CDS             3058..>3404
                     /locus_tag="PLB25_18455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013592681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOE43587.1"
                     /translation="MKVLLAVDGSQYSKKMLAYLVNHDDLFTSAHDYVLFTVQSAVPP
                     RARSAVGKEIVQKYYTDEAEKVLAPIDKFLQRHGLIAKSSWKVASPGPAIAELATEGK
                     FDLVVMGSHGHGVL"
BASE COUNT          844 a          948 c          877 g          735 t
ORIGIN      
        1 atgcggacaa attctgtgat catcgccaag gctaggccaa tcagcatcgc cttgatgatg
       61 tccatcttga tccaagaaat ccacaaatca ctgaatatca aaatgagggc gagaggaatg
      121 atcggcaata tggcgtagta catgggtgga attttttcca cttcgccgtc tttgccctta
      181 ggtgcaatca aaccggtgac ttgaaccacg tgtccatcgc gtttgtccat ccatttttgt
      241 gtaaagaaat gcagaaccgc aacgacagga atcaccgcca gggccactgg aacctggtac
      301 ttcacccagt atttgacggg atcaatgcca gccgttgtgg cggccagaat cgtgcctgta
      361 tcgctgggac tccagtcaag gcacagggtt gtggcaatca ccgcaacggc cgacaagcga
      421 ctgacaccga ggctgatcaa aaccgggaag gcggtcacca tcagcagcat ggccaagccc
      481 gatgcgctgt taatggccaa tcccaggcac attcccataa tccagacacc ggccatgaca
      541 aggtaggggg atcgcagcgc aacaagtggc ctgatggtca acttgacaag ggccttgctg
      601 gcaccaatat ggtccatata acgtgcaaaa ccgccaacag ccatgatgtt gagacccaag
      661 cctccggccc tggatgcgaa agttttcttg ataaattcaa aggcatcaaa cagcaccagg
      721 ccagtgcttt ccttggcagg caatatcgtg cccaaaccaa gaagtacggc caaaaacatc
      781 aacgccagac ctgccataaa caaaacaggt tggggcttga actttttgat gatcagatag
      841 cctgcccaaa aagtcacaat caaagatagg gtaacgccca aaatatcacc tcgaacgggt
      901 agttattgtt gaaaaacagc atcacgcaag gcgcttgctg ttgacttgta tctagggggt
      961 gattgacgaa actgctcgca tccgagcaag tcaatcaaaa cgaatcagct gacacagtgg
     1021 ccaattccgc ccatcacact ttgtagcgtc aacaacatcg ccgagcccag gtggcctcgg
     1081 caaacaaccc acgccagact gagtgaaccg atcgcatgcc tgtcttgcga ttcggtgccg
     1141 atcgtgaagc ctcctcgggc catgcattta aatcaacgac gttcattctc ttattcatgc
     1201 gtcgtcactt ctgtcccaac gttgtcacat cgggatgttg taagttaatg atttgcttgt
     1261 taaatttggt gtttgagtct ggtctcgatt tgaacgtcgt cgagcgtcaa actgtcggtt
     1321 ttttgcggat ttccgcccaa gaccgtcatg caccaaggca agctcgtatt tgcgcaactc
     1381 atgctctatc tgccactgag cacgtttcgg cgctgtgtgg ccgaccacaa cggcgaacac
     1441 aaggtcaagg acttctcttg cctggatcaa ttcttcgcca tggcattcgc ccagttgacc
     1501 taccgcgagt ccctgcgcga catcgaagtc aaccttcgcg ctcagggacg tcggctgtat
     1561 cacatgggct ttcgttgcca gacgatttcg cgcaacaccc tggccaacgc gaatgccaca
     1621 cgtccgtggc aaatttatgc cgacttcgcc aagcatctga ttggcttggc gcgacccctg
     1681 tacgccacgg agccgtttgg cgtggaactc gatgcagcgg tctacgcctt cgatgccagc
     1741 actatcgact tgtgtctgtc ggtgtttgcc tgggcaccgt ttcgctcgac caaggcggcc
     1801 atcaagttgc acacgctgct ggacttgaga ggcaatatcc ccagtttcat tcacatcacc
     1861 gacggcaaga cgcacgaggt caatgtgatg gactccctgt tgctggagcc gggagcgttt
     1921 tacctgatgg atcggggcta tctggacttc gccagattgt tcgtgattca cgaggcgcaa
     1981 gccttctttg tgacgcgctc caagagcaac accaagttca agcggcgcta ctcgcatccg
     2041 gtggatcgaa tcaagacgaa agtactgtgc gatcagaccg gcgtgctgac aacctatctg
     2101 tcgagcaagg actaccctac aacgatgcgc cgcgtggtga tcagggacga cacgggaaag
     2161 cgattggtat ttctgaccaa caactttgcc ttgaagcccg agttgattgc tgatttgtac
     2221 cgccagcgct ggcaggtgga gttgttcttc aaatggatca agcagcattt gcgtatcaag
     2281 gtcttctttg gcaccggcga gaacgccgtc aagacgcaga tatggattgc catttccacc
     2341 tacctgctga tcgccattgc caaaaaacgc ttgcatcttg atcatcacag cctgtacgaa
     2401 attctacaaa tcctgagtct gtccatgttt gaaaatatcc caataaatca actacttaaa
     2461 ccaccctcga caaattcaga tgcggatttc gagtcaaatc agctcgctct cctatgaaga
     2521 acgttgggac actactgatc cagcacaaga accgacgacg ccaccgcaat cgccagcagc
     2581 cagttacccc atgccaggtc aacggtatcg aagatggtct gcgccgggcc ccagtgaacc
     2641 gccagcacct gcatgcccag aacggcgccc aaggcgagcc acagcttgac gttgctgaaa
     2701 aagttcgtgt tgaacactgt gctgtgctcc gagcgggcgt tgaaaacatt gaagaactgg
     2761 aacagcacga aggttgtgaa agccagcgtc agggcatgag actggccgtc acgggccaga
     2821 gcgtactgaa acatggagcg cccgaacaac gcacatcttg tgggcgggcg agacaccggc
     2881 gaaaacggct gtggcgtcca agctcgcggc caaggcggcg gcgtccagca cgtgcagttc
     2941 cggtccacca tctgacatgg cgatcggcca aatactatca gtctgtaggg acattgcatt
     3001 gcatccctgg gtggaatcgg tacgcttgca ttcaaacggc caacccaagg agtcaatatg
     3061 aaagtcctgc tcgctgttga tggcagccag tacagcaaaa aaatgctggc ctatctggtc
     3121 aatcacgacg accttttcac ctcagcccac gactacgtgt tgttcaccgt ccaaagtgct
     3181 gtacccccca gggcgcggtc tgcggttggc aaagagattg ttcaaaaata ctataccgac
     3241 gaagctgaaa aggtgctggc tcccatcgac aagtttctac agcgccacgg actgatcgcc
     3301 aagagcagtt ggaaggtggc gtctcccggc cccgccatcg ccgagttggc taccgagggc
     3361 aagtttgacc tggtggtgat ggggtcgcac ggccacgggg tgct
//
LOCUS       DAOSLL010000538         3394 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24376_length_3394_cov_4.406409, whole genome shotgun sequence.
ACCESSION   DAOSLL010000538 DAOSLL010000000
VERSION     DAOSLL010000538.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3394)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24376_length_3394_cov_4.406409"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(483..1703)
                     /locus_tag="PLB25_18460"
     CDS             complement(483..1703)
                     /locus_tag="PLB25_18460"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HOE43588.1"
                     /translation="MTLQIDLKSAVLALSDALDLVGVNDFQHGKRVAMIARKSAVLLG
                     LPEAQLDNLVCACLLHDIGVSSTAMHRRLIHDSEWNDRHAHCERGHELLQPFAHLSLL
                     ANAVRYHHTPWVEMPSGLSEADALNSNLILLADHVDSVLLREGLENPLLQREAICAEI
                     QAMAGHLLAPEVVRAFLGACANEAFWIALVPRHVHFYLSELPLANWQITIEFPQVRHV
                     ARLVSSIVDAKSHFTASHSLGVAQLSRHLGECMGLHADALVQVEIAGLMHDVGKLCVP
                     DEIIDKPFVLTPMEFSVMERHSFETYQILRHIPGMEMVAEWAAFHHETLNGRGYPFRH
                     KAERLSLEARIVAVADVFQALAQARPYRKPQPVEVIPGILSLFVAKGALDPDVVGVAS
                     EDLQASWLAATQTL"
     gene            1781..2443
                     /locus_tag="PLB25_18465"
     CDS             1781..2443
                     /locus_tag="PLB25_18465"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008161580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE43589.1"
                     /translation="MTTKSQAAKELAELTQRTVAYYNQNADDFFVGTRDHDVRQNMDA
                     LLQFIEGAVPHSILDFGCGPGRDLKALSALGHRAVGLDGAARFADMARAYSGCEVWHQ
                     DFLALDLPEAHFDGVFANASLFHVPVRALAQVLRQLHDTLKPRGVLFSSNRRGFNDEG
                     FHGERYGAYHDLAGWRHHMSRAGFTELLHYYRPAGLPIEQQPWLASVWRKAEPGCTDK
                     TV"
     gene            2565..3239
                     /locus_tag="PLB25_18470"
     CDS             2565..3239
                     /locus_tag="PLB25_18470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013900787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HOE43590.1"
                     /translation="MIQTLKIDFVSDISCPWCAIGLAALEQALNQLRDEVAVDIHIHP
                     FELNPQMPAGGQDISEHLTQKYGSTPEQQMQIRATIAQRGADVGFTFNPSGRGRIYNT
                     FDAHRLLYWAGLGSVPGQLALKKALLVACHAQSQAMESPDVLLNAVASAALDVPRARA
                     ILASDEFAAEVREAEAFYTGQGIHSVPAVVINDRHLISGGQPAAVFAQALRQIAAEQS
                     PKRKPN"
BASE COUNT          749 a          975 c          962 g          708 t
ORIGIN      
        1 cctatgctca ggtactctgc ttgaacgcac caagatgtgc ggcagaccag gatgtgcgtg
       61 tgccacggat ccaaccgctc ggcatggccc ctacattgag tggggtcaca tgcgtggggg
      121 caagctagtt catcgccaga tatctgctca gcaggccctc tcaattcgtt tggccatcga
      181 caacttccga agggctaaga agttaatgcg agagtgggag acagaaacgg aacgtctgat
      241 agacgctgag caacagatcc aaaactgagg ccagagccac tcagtaattc ggattaaaca
      301 cgctcaaaga tgtgcggaat gtgagttttg attcgcgatt gctatatctt tagctccctt
      361 gaggccgcat tccaaggtac tacgccacag cccagcttcg gctaaattct cccgctagcc
      421 caacatgggg tgagtagtca caatctggca tctataactt gttatagccg gattaaccac
      481 gctcaaagtg tttgcgtggc ggccagccaa ctcgcctgca ggtcctcgct ggccacgcca
      541 accacatccg gatcaagtgc ccctttggcg acaaataagc tcaggatgcc ggggatcact
      601 tccaccggct ggggcttgcg atagggacgt gcctgcgcca gcgcctgaaa tacatccgct
      661 acggcgacga tgcgagcttc aaggctgaga cgctcagcct tatggcgaaa gggataacca
      721 cggccattga gggtttcatg gtgaaaggcc gcccattcgg caaccatctc cattccgggg
      781 atatggcgca ggatctgata ggtctcgaaa ctgtggcgct ccatgaccga gaattccata
      841 ggggtcagca caaaaggctt atcgataatt tcgtcgggca cgcacagctt gcccacatca
      901 tgcatcaggc cggcaatttc cacttggaca agggcatcgg cgtgcaatcc catgcactct
      961 cccagatgcc gggaaagctg cgccacaccc agtgagtgcg atgcagtgaa gtggctcttg
     1021 gcatcgacaa tgctcgatac caggcgcgcc acatggcgaa cctgcgggaa ctcgatggtg
     1081 atctgccaat tggccagcgg cagttcggag aggtaaaaat ggacatggcg cggcaccagc
     1141 gcaatccaga aagcctcgtt ggcacaggcg ccaagaaagg cgcgcacgac ctcgggcgcg
     1201 agtaggtgac cggccatggc ctgaatttcg gcacagatcg cttcccgctg caaaagcggg
     1261 ttctccagcc cttcgcgcag cagcaccgag tccacgtggt cggcaagaag tatcaggttg
     1321 ctgttgagcg catcggcctc actcaggccc gacggcattt ctacccaggg cgtgtggtgg
     1381 tagcggacgg cgttagccag cagcgacagg tgagcaaaag gctgcaacag ctcatgcccg
     1441 cgttcgcaat gggcatgccg atcattccat tctgaatcat gtatcagccg ccggtgcatg
     1501 gcagtggatg aaacgccgat gtcatggagc aggcaggcgc ataccaggtt gtcgagttgc
     1561 gcctcaggca agccgagcag aacggcgctt tttcgcgcga tcatggcgac ccgcttgccg
     1621 tgctgaaaat cgttgacccc gaccagatca agggcatctg acaaggccag cacagcgctt
     1681 ttgaggtcga tttgcaaagt catcgatcat ttgctccaag aatatttgaa cagcatagca
     1741 ttgctctgtc tcaaaatagg ttcttttacc ccgccgtccc atgaccacca aatcccaagc
     1801 agccaaagaa ctggctgaac tgacgcagcg caccgtggcc tactacaacc agaatgccga
     1861 cgattttttt gtcggcaccc gcgaccacga tgtgcgccaa aacatggatg ccttgctgca
     1921 gtttatcgaa ggggcggtgc cgcacagcat tcttgacttt ggctgtggcc ctgggcgtga
     1981 cctgaaagca ttgagcgcac tggggcaccg ggcagtgggg cttgacggtg cggcgcgttt
     2041 tgccgacatg gcacgtgcct acagcggctg cgaggtgtgg catcaggatt ttttggcgct
     2101 tgacttgcct gaggcgcatt ttgacggtgt gtttgccaac gcctcgctgt ttcatgtgcc
     2161 ggttcgggcg ctggcgcaag tgctgcgcca gttgcatgac actctgaaac cacgcggcgt
     2221 gttgttcagc tcaaaccggc gtggcttcaa tgacgaggga tttcatggcg agcgctacgg
     2281 ggcttaccat gacctggccg ggtggcgcca tcacatgagc cgcgcagggt ttaccgagtt
     2341 gctgcattac taccgaccag ccggtttgcc cattgagcag cagccctggc tggccagcgt
     2401 gtggcgcaaa gcggagccgg gttgcacaga caagacagta tgattggtgc agctttcgtc
     2461 aaaacaggtc gctcactctg ttgcgcaccc gctttcgcgg gtgagaacgg gtttgtgcaa
     2521 gacagcacaa tgccttttcc aaaccccata gccggataca ccccatgatc caaacgctca
     2581 aaattgactt tgtctctgac atttcctgtc cgtggtgcgc cattggccta gccgcactgg
     2641 agcaggcgct gaaccagttg cgtgacgaag tggccgttga catccacata catccgtttg
     2701 agctcaaccc ccaaatgccc gcagggggtc aggacatcag cgagcatttg actcaaaagt
     2761 atggctccac cccagagcaa cagatgcaga ttcgggccac cattgcccag cgcggggcgg
     2821 atgtcgggtt taccttcaac ccctctggcc gaggccgcat ttacaacacc tttgatgcac
     2881 atcgcctgct gtattgggct ggccttggct cggtaccggg tcagcttgcc ctgaaaaaag
     2941 ccttgttggt cgcctgccat gctcaaagcc aggccatgga gtcgcctgat gtgcttctga
     3001 acgctgtggc aagtgccgcg ctggacgtgc cacgcgcccg agccattttg gccagtgatg
     3061 agtttgctgc cgaggtacgt gaagccgaag ccttttacac cggccagggc attcattcag
     3121 tgcccgccgt cgtcatcaat gaccgccacc tgatctcggg tggccagcct gctgccgtgt
     3181 tcgctcaagc cctgcgccag attgccgctg agcagtcacc aaaaaggaag ccaaattaac
     3241 tttgcttctc catcaaacag cgcgttacta gaggcatcgc aacaacgcac tcggagattg
     3301 aaatgaccaa cgcaacacaa accttcgcct cgcagaatct tgattttatg tggagcgaat
     3361 tgttatgcgg agttcgaggc attgccagcg ttgc
//
LOCUS       DAOSLL010000539         3391 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24401_length_3391_cov_5.253597, whole genome shotgun sequence.
ACCESSION   DAOSLL010000539 DAOSLL010000000
VERSION     DAOSLL010000539.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24401_length_3391_cov_5.253597"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="PLB25_18475"
     CDS             complement(<1..1253)
                     /locus_tag="PLB25_18475"
                     /inference="COORDINATES: protein motif:HMM:NF015859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE domain-containing protein"
                     /protein_id="HOE43591.1"
                     /translation="MKRWHIPWYLWALAVNVIVVGVGFSGTWLMYRAMSTTINLQSSD
                     RLNASGRGILEAFNKDLSATVEMVHATSLMVMSHEKLTPDGWRVFTAGISRRLNLIAG
                     IAVLEWAPTVNAMQRSTFERDVRQATQINFGIVEFSNGAWVPAAPRDMYRPRLYATPE
                     FNERIGEDLALLPEREEACAQARDSGEVVASAAYLPVDSFARAASVEANRESLAMSVS
                     APVYRGGVRLNPTLRMRNMVGCVSALVKVSDLMEASAFRADKAHVDLQVFDVSGNRQL
                     LFSQRGDNRELSSQDIAHYRPPGNSLQFSVTVASRSWEVVLSPRAVFYADEDRRSLHW
                     SAATGIITTLLMSVAVFLLSRSNHLTAQARSKAEAADRSKSDFFANMSHEIRTPMNAV
                     LGISHLLQNWTVRSSPLRVQAAIGCA"
     gene            complement(1435..2418)
                     /locus_tag="PLB25_18480"
     CDS             complement(1435..2418)
                     /locus_tag="PLB25_18480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007859547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE43592.1"
                     /translation="MLDTLLRAVAVLTLVWSMGVQAQAVVLYSSNNAETIAIAVGASK
                     KKQADLQIKSVTGGTGTLLKRIKAEAANPVADIFWSGGVGTLAAFPEVMEPYESPDAK
                     AIAPQFRDSKGRYIGVNLHVMVLIVNEKMLRGLPAPKTWSDLMKPEWKGKFAVTDPSK
                     SGTAFIQVYGLLKQFGRDGLEKITSNAVVVHSSGQVSKSVVAGEYPVGITTESSVQGY
                     INDGHKEIKMIYPNEGTYLGAEGMFLVKGAKNARAAKQLYDLLLSKPVQQSLLVEKFR
                     RPTRSDIAVSKLTGLPDLANLKVFPLSLQVATEEYEQLIALWNLTQTKARR"
     gene            complement(2715..2931)
                     /locus_tag="PLB25_18485"
                     /pseudo
     CDS             complement(2715..2931)
                     /locus_tag="PLB25_18485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003076814.1"
                     /note="catalyzes the transfer of electrons from NADPH to
                     thioredoxin; FAD/NAD(P) binding; frameshifted; incomplete;
                     partial in the middle of a contig; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
     gene            complement(2933..3250)
                     /locus_tag="PLB25_18490"
     CDS             complement(2933..3250)
                     /locus_tag="PLB25_18490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43593.1"
                     /translation="MSAGINLGYTERSGKSTASGIAPTNLFLGGLQMMWAYRMAQLAG
                     DRLAHTYDTYIYHLPDGGLLRQKTYAGMVLEAGDAWSSPARAETKRNGTLFFAVDSKI
                     GDI"
BASE COUNT          745 a          965 c          910 g          771 t
ORIGIN      
        1 gcgcagccaa tggcggcctg aacacgaagg ggggaactac gcactgtcca gttttgaagc
       61 aagtgactga tacccaggac cgcattcatc ggcgtacgta tttcgtggct catgttggcg
      121 aaaaagtccg acttactgcg gtctgctgcc tcagctttgc ttcttgcttg tgccgtcagg
      181 tgattgcttc ggctcagcaa aaacacagcg acactcatga gcaaggtggt aatgatgcct
      241 gtcgcggctg accagtgaag actgcggcgg tcttcgtcgg cataaaaaac ggctcgcgga
      301 ctgagcacca cctcccacga ccgggacgcc accgtgaccg agaactgcaa gctgtttcca
      361 ggtggccgat agtgcgcaat gtcttgtgag gacaactcgc ggttgtcgcc tcgctgcgaa
      421 aacagcaact gacgattgcc tgaaacatca aatacctgca gatccacatg cgctttatct
      481 gcgcgaaatg ccgaggcttc catcaagtcg gaaactttga ccaaagcaga gacgcaaccc
      541 accatgttgc gcatcctcag cgttgggttc aggcgcacac cgccacggta gaccggcgcc
      601 gagaccgaca tggcgagaga ctcgcgattg gcctcaacgc ttgctgcgcg ggcaaaagag
      661 tccaccggta ggtaggcggc cgaagccacg acttcaccgc tgtcccgcgc ttgggcacac
      721 gcctcttccc gctctggcag gagcgcaagg tcttcaccga tgcgttcatt gaattcaggg
      781 gtggcgtaca gcctgggtcg gtacatgtct cttggcgctg caggcaccca agcaccgtta
      841 ctgaactcga caatgccaaa gttaatctgg gtggcttgcc gcacatctct ttcaaaggtc
      901 gagcgctgca tcgcattgac agtgggtgcc cactcaagca ccgcaatgcc cgcaatcaga
      961 ttcaagcgcc gactgatgcc ggccgtaaaa acgcgccatc catcgggcgt caatttctca
     1021 tgcgacatca ccatcaaaga cgtagcgtga accatctcca cagttgcaga caagtccttg
     1081 ttaaacgcct ccaaaatacc gcggccgctg gcgttcaaac ggtcactgga ttgcaggttg
     1141 atggtggttg acatggcgcg gtacatcaac caggtgccag aaaatccgac accaacgacg
     1201 atgacattga ccgccaaagc ccagaggtac caagggatgt gccagcgctt catggattta
     1261 ttcgatttcc ataccaatcc gacatgttgt tgctttgcct tgccgtgctg tagcactttc
     1321 cgcggttccg cgcctaaagt cgcttcgatc tggaaacgga gtcagtcgct attgagcgcg
     1381 atcctgatcc gcatcgcgct caagtagact gagcatccga caagcatgca aaggctatcg
     1441 tctcgccttc gtttgcgtca ggttccaaag tgcgatgagt tgctcgtact cttctgtcgc
     1501 cacctgcaaa ctcagcggaa acaccttcag attggccaag tctggcaacc ccgtcagctt
     1561 ggataccgca atgtcactgc gcgtgggacg gcgaaatttc tccaccagca gtgactgttg
     1621 cacgggcttg ctgagcaaca ggtcgtaaag ctgcttggcc gcgcgggcat ttttggcgcc
     1681 cttgaccagg aacattcctt ccgcgcccag ataagttccc tcatttgggt aaatcatttt
     1741 gatttccttg tggccgtcat taatataccc ttggactgat gactcggtgg tgatgcccac
     1801 cgggtactcg ccagcgacca cacttttgga aacttgacca gaactgtgaa cgacaactgc
     1861 gtttgaggtg atcttttcca ggccatcgcg gccaaattgc ttgagcaatc cgtatacctg
     1921 gataaaggca gtgccagact tggaggggtc ggtaacggcg aacttgccct tccactctgg
     1981 cttcatcagg tcagaccagg tcttgggtgc gggcaagcct cggagcattt tctcgttgac
     2041 aattagcacc atgacatgca aattgacccc gatgtagcga cccttgctat ctcgaaattg
     2101 cggtgcaatg gctttggcat cgggcgattc gtaaggttcc atcacctcag gaaatgcggc
     2161 aagggtgcca acgccaccac tccagaaaat atccgccaca gggtttgctg cttcggcctt
     2221 gatgcgcttc aacaaagtgc ctgtgccacc ggtgactgac ttgatctgaa ggtcggcttg
     2281 tttcttcttg gaagcgccca ccgcaatggc aatggtctct gcgttattgg acgaatacag
     2341 cacgacggct tgggcctgaa cgcccatcga ccaaaccaag gtgagtacag cgacggctct
     2401 gagtaatgta tctaacattt tttttgctcc ctacttcaga agaaacagat gcccttgcac
     2461 gctatgctcg acaagcccac cggcacaaaa atcacggcat ccaccacgcc gtcaaaattt
     2521 catgtctgtg tttgattggg gcaccgacca tccccccact gagggatggc ggattttagc
     2581 gcagtggtgt tcaagatttg ctgcacaagc gtgacgcaaa gccccctggg aaaggggtgc
     2641 catttgctct gcctgactga ttgatcgcga ccttagtcgc tgtgcccgtt ggtgccaaac
     2701 ctgaatctgt tttcctagaa ctgctgggca tctggcatcg ccatgcagtc ccgtgcccgc
     2761 gcttgttgtg gcctggcggt agacgtgatc ctggacatca ccctgcggca aaatgcccgg
     2821 cacgctggtt tgcgtggcaa agcctttgag cccgccctgg gtcacgatgt agcagttttc
     2881 cttggcgagt tgagcctgga aaatcccgat gttgggggtg tggccgatgg cattagatgt
     2941 caccgatctt gctgtcgacg gcaaagaaca aggtgccgtt tcgtttggtt tcggcccgag
     3001 ccggactgga ccaggcatcg cccgcttcca gcaccatgcc cgcataggtt ttttgcctga
     3061 gcaggccccc atcaggcagg tgatatatat aggtgtcata ggtatgggcg agtcgatcac
     3121 ccgccaattg cgccatccgg taggcccaca tcatttgtaa accgccaaga aacaggttgg
     3181 tgggcgctat gccagaggcg gtacttttac cggatctctc ggtgtatccc agattgatgc
     3241 ccgcactcac gatgtgtgac cgcagggacc cagccagacg agcgcttaac cggtagctgc
     3301 ctattgatct tttcataatt catgacaacc attcaccgcc tcgtgcgtta ttgcttcaac
     3361 gagttgattg gagagatgac gaatggctgc a
//
LOCUS       DAOSLL010000540         3385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24473_length_3385_cov_2.436637, whole genome shotgun sequence.
ACCESSION   DAOSLL010000540 DAOSLL010000000
VERSION     DAOSLL010000540.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24473_length_3385_cov_2.436637"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="PLB25_18495"
     CDS             <1..1270
                     /locus_tag="PLB25_18495"
                     /inference="COORDINATES: protein motif:HMM:NF013235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HOE43594.1"
                     /translation="AIVPKTGSITRVGRQAYTIMMMLAREQCVEDEKTGMFTAPLNSV
                     IRGFSGSMGTFEDLKRHLRSMVTHVVEWQSPSPGETEEWGACGLLSQVSTVKKKGEIW
                     LSWAYPPALRQEMMSPLRFAQIRRSTIAQFRTHAGLALYEICARYKDNPSHLTSKQHW
                     HWWLPVLSGKPAPKEIKTQFRFFNRDTLKPAVEEVNEVSELIVRVIEIKVGRTIEFIQ
                     FEVQLKPEVSAKSVKALDLSKVAKAHQLGIDSEIAEDLYIRHGELAFSKAIERLEARL
                     AMPGPPVLSRHAYLKSLLVGKVLDTPKPYLAPEKDVAEAATAVVNPVFEKQASFQERE
                     SERVKAVRAEIEALDKVSLEHLLGRLKQQFVERQMSAPVMKRLEEGKWQSALILGELI
                     RCYWKQTRGTDWTSIEELPVAERVEQAGLF"
     gene            1857..3191
                     /locus_tag="PLB25_18500"
     CDS             1857..3191
                     /locus_tag="PLB25_18500"
                     /inference="COORDINATES: protein motif:HMM:NF023299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HOE43595.1"
                     /translation="MTSDDSKHAYLSSDQLCVGVFVLLDLPWFKHDFAVNSFKVRTED
                     QLRELRQLNLERYRYDPARSDPLPELSPLGEVLTDSTQSAEGAQSAQSAAQPGQAVGS
                     SVGNAGKDQLVAMIEQRRANIAQVEMAFAKAATVMKSMNRNIYIRPQETLEEMGGLVE
                     DMVKAFLNSADATLHVMGDKSGGEDVYFHSLNVTILAMMLAKGLNFSPDMAREMGIGA
                     MLHDIGLTEIPDRIARKPVIESNKAERNLRATHVELGMALGRRIGLSEHSLAVVAQHH
                     ECNDGSGYPMGLKGSEMSTAAKVVALVNFYDNLCNPADMGRAMTPHEALSYMFAQCRS
                     KFDAQALQLLIRSLGVHPPGSILLLSNQALAVVTSVNPKKPLRPWVLVYDENVPKDQA
                     LTLNLEQEPQLSVIKSIRPGQLSPKVSAYLSLRKRVTYYFNSSNTEEQTGAR"
     gene            3191..>3385
                     /locus_tag="PLB25_18505"
     CDS             3191..>3385
                     /locus_tag="PLB25_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43596.1"
                     /translation="MTIDNLLLDVSSEMLMLLDARSMVILGANQTAHEHLKYPPGTLV
                     GQSMSDIECDISDLFFWDEMR"
BASE COUNT          841 a          886 c          935 g          723 t
ORIGIN      
        1 ggctatagtt ccgaaaacgg ggagcattac gcgcgtgggg cgacaggctt acaccatcat
       61 gatgatgctt gcgcgcgaac aatgcgtgga agacgagaag accggaatgt tcacggcgcc
      121 tctgaacagc gtaattcgag gcttttccgg atccatgggt acgtttgagg accttaagcg
      181 ccacctccgg tcgatggtga cacacgttgt cgaatggcag tccccctcac caggtgagac
      241 agaggagtgg ggcgcctgtg gattactgtc gcaggtcagt accgtgaaaa aaaaaggtga
      301 aatctggctt tcgtgggcct acccaccagc actccggcaa gaaatgatgt caccgcttcg
      361 ctttgcgcaa atccggcgtt ccacgatcgc gcaatttcgg acccatgccg ggctcgcact
      421 ttacgaaatt tgcgcaaggt acaaagacaa tccgtcccat ctcacaagca agcagcactg
      481 gcattggtgg ttgccggtcc tgtcaggaaa accagcaccc aaggaaatca aaacacagtt
      541 tcgatttttc aacagggaca ccctaaagcc agccgttgag gaagttaacg aagtaagtga
      601 gctgattgtt cgcgtgattg aaatcaaggt tggccggacc attgaattta tccagtttga
      661 agtgcagctc aaacctgagg tgtcggcgaa gtccgtcaag gcacttgatc tgagcaaagt
      721 cgccaaggcg caccagttag gtatcgattc ggaaatcgct gaagatttgt acatccgcca
      781 cggcgaactc gctttttcca aggccattga acggctggaa gcacggttgg ccatgcctgg
      841 gccgccggtg ctttcaaggc atgcctatct caagtcactg ctggtcggta aggtgctgga
      901 tacgcccaag ccctatttgg caccggaaaa ggacgtggct gaggccgcaa cagcagtggt
      961 taatcccgta tttgaaaagc aggccagttt tcaggagcgc gagagcgaaa gggtaaaggc
     1021 ggtgcgcgca gaaattgagg ccttggataa ggtgtctctg gagcacctgt tgggtcgcct
     1081 caagcaacag tttgtcgaac gtcaaatgtc ggcgccggtc atgaagcgac tggaggaggg
     1141 caaatggcag tcggctctga tcctggggga actcattcga tgctactgga agcaaactcg
     1201 cggcaccgac tggacatcaa ttgaggagtt gccagttgcc gagcgggttg agcaggcggg
     1261 cttgttctga acggacgact taaccggcgt aaagaaatga aactggtcgc cgatgttgac
     1321 gccgattcaa atctgttttt cacataaaca attacgccac cggatcacgt ccagggcggc
     1381 aatcgatgtt ggaaatccgg ctgaactggc cagcaacatc caaagattgg ttcgtcgttc
     1441 atctttcacg acgctagtgc cgttgacgtg gaagtagcca accgccaggc gaggtttgaa
     1501 gccagatcaa tatcggcgtc taccagaaga gtttggaaga cagctgtctg tggtacagcg
     1561 gtcacagaga ttgggatatt gggtgactgt tgatgaccaa cagcgggtgc tcccaggtga
     1621 cagcttgcgg gcagcagaaa acatgtcttg tagcccgttg caaagaatag ccgctccttg
     1681 attagccaga tcgctggaac agagagggcc gcaagaacaa aaagcgaggt gctttgcaaa
     1741 acctatgatg ccgttcaatt caatcttggt cgttcagttg ctcagacgct taaaatgacc
     1801 acactccatc cagtccagga aattgtcacc tctctgacgg tcttcaactg tcctttatga
     1861 cttccgatga ttcaaaacac gcctatttga gctcggacca actttgtgtt ggtgtttttg
     1921 tccttctgga tttgccttgg tttaaacacg actttgccgt caatagtttc aaagtccgca
     1981 ccgaagacca attgcgtgaa ctgcgccaac tcaatttgga gcgctaccgc tatgaccccg
     2041 cgcgcagcga cccgttgcca gaactgtcgc ccctgggtga agtcctaaca gacagcacgc
     2101 aaagcgccga gggcgcccag agcgcccaga gcgcagccca gcctgggcag gctgtgggca
     2161 gctctgtcgg gaatgccggc aaggatcagt tggtagccat gatcgagcaa cgccgcgcca
     2221 atattgcgca agtagagatg gcctttgcga aagccgctac ggtcatgaag agcatgaacc
     2281 gtaacattta catccgcccc caggaaacgc tggaagaaat gggtggcctg gtggaagaca
     2341 tggtgaaagc ttttctgaac agcgccgacg ccaccttgca tgtgatgggc gacaaatcgg
     2401 gtggcgaaga tgtatatttt catagtctga acgtcaccat actggcaatg atgctggcca
     2461 aaggcctgaa ctttagtccc gatatggcgc gtgaaatggg catcggtgcg atgctccacg
     2521 atattggtct gacggaaatt cctgaccgta ttgccagaaa acccgtcatc gaatccaaca
     2581 aggccgagcg aaatttgcgc gccacacatg tcgaacttgg catggccttg ggccgacgca
     2641 tcggactctc agagcactcg ctggcagtgg tggcacagca ccacgaatgc aacgacggca
     2701 gcggctaccc aatgggcctc aagggcagtg aaatgagcac ggccgccaaa gtggtggcgc
     2761 tggtcaattt ttacgacaac ctgtgtaacc cggccgacat gggccgtgcg atgacacccc
     2821 acgaagcgct gtcctacatg ttcgcgcagt gccgcagcaa atttgacgcc caggccctgc
     2881 aattgctcat ccgcagcttg ggtgtgcacc ctccgggctc aatcttgctg ttgtcaaacc
     2941 aggctctggc ggttgtgacc tcggtcaacc cgaaaaaacc gctgcggccc tgggtgcttg
     3001 tttacgacga aaacgtccca aaggatcagg cactcacact caacttggaa caagaacctc
     3061 aactgagcgt cataaagtct atccgaccag gacagctgtc acccaaagtg agcgcttatc
     3121 tcagcctgcg caaaagagtt acctattact tcaattcaag caataccgaa gaacaaaccg
     3181 gggcccgctg atgaccatcg acaacttgct tctggatgta tcgagtgaaa tgttgatgtt
     3241 gcttgatgct cgatcgatgg tcattctggg tgccaatcag accgcgcacg aacacctgaa
     3301 atatccacct ggcacactcg ttggccagtc catgagcgac atcgagtgcg acatttccga
     3361 cttgtttttc tgggatgaaa tgcgt
//
LOCUS       DAOSLL010000541         3369 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24630_length_3369_cov_4.589016, whole genome shotgun sequence.
ACCESSION   DAOSLL010000541 DAOSLL010000000
VERSION     DAOSLL010000541.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24630_length_3369_cov_4.589016"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1615)
                     /locus_tag="PLB25_18510"
     CDS             complement(<1..1615)
                     /locus_tag="PLB25_18510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43597.1"
                     /translation="MNSIRIGKSFLLALIMLPLWLANHRTKPEQSHGLLSLGIMLGLA
                     SASLATVWERTAFTGLLDFSSDYRTTALFWEMHVGGAALDGFLALTVPVALRELMIAR
                     SHARLGLAVTVLVLAAYACLTTFSRGVYLAVPVAVALFSVLSTAQRSTGNDLTAVTQG
                     STGSAWIGGQVRQDEAVGRNDSAGWLPGIVLVAAFGFGAAWMFQSSGYRGMAALLTAT
                     ALMLPLAKVMRGFSRRQWVMGTMFGAVMILLATGLALVLPKGAYIVWALTAALTLGGL
                     IALRPPTKPTSLAGPLVFASFVATLVGVALVANHWGDAPGLMHAVPVLLALLTLSVVV
                     GRRRASVWPDHIRWQISVVGVMALAAILVGIFGGGAYMGGRFATGGQDMEGRFAHWKL
                     GRDMLQTSEQWWLGKGLGRFPASYFMLGKPQEHTGDYRIKQEGDNTYLTLAGGLHING
                     WGEIFRVSQRVSEPGKAPVVTAQVRVEKDVGLHFEVCEKHLLYGHSCLTKQVGIKGKP
                     GEWQDLRVVLDGDGASRGAWYAPRLLMFSM"
     gene            1645..1980
                     /locus_tag="PLB25_18515"
     CDS             1645..1980
                     /locus_tag="PLB25_18515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43598.1"
                     /translation="MKNHPHLQSRELSLDLLKQQTALSICRNAGEYCGGSLQPYKIPD
                     ARNRQQRQPKQACQVLQKQANLSKVQSQPKATTPVAPQSRHWAIKKTGCQQLQKQRST
                     PDGQPDSAW"
     gene            complement(2830..>3369)
                     /locus_tag="PLB25_18520"
     CDS             complement(2830..>3369)
                     /locus_tag="PLB25_18520"
                     /inference="COORDINATES: protein motif:HMM:NF013752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOE43599.1"
                     /translation="SPTTFYFSMQLPHKTTKNHKPALTLSWKKEFIQGTMATQPGVYC
                     LRTNVLDWDEQKLWQTYSTLTDIESVFRSLKGELGLRPVFHQKENRSDGHLFMTVLAY
                     QCVQVIRKTRKEQGIHDSWATLRNTLSVQQRVTASMQRSDGRSIHIRKSTKAEPALMR
                     IYQALGISEAPGGTKKLVA"
BASE COUNT          865 a          961 c          800 g          743 t
ORIGIN      
        1 ccattgaaaa catcaatagc cttggcgcat accaagcccc gcgagacgca ccatcaccgt
       61 ctaacaccac acgcaggtct tgccactcac caggtttgcc tttgatgccc acctgcttgg
      121 tcaagcagct atgtccgtaa agcaagtgct tttcgcacac ctcaaaatgt agacctacgt
      181 ctttttccac gcgcacctgg gctgtcacaa caggcgcctt gccaggctca gaaacccgct
      241 gcgaaacccg gaaaatttca ccccaaccgt tgatatgcaa accaccggct agggttaggt
      301 aggtgttgtc gccctcttgt ttgatgcggt agtcacccgt gtgctcctgg ggtttgccaa
      361 gcatgaaata gcttgccgga aagcgcccaa gccctttgcc cagccaccat tgctctgatg
      421 tttgcaacat atcgcggccc aacttccagt gcgcaaagcg gccctccatg tcttgcccac
      481 cggtcgcaaa tctgcccccc atataggcac cgccgccaaa aattcccacc aaaatcgcag
      541 ccaatgccat gacacctacc actgaaattt gccaccggat gtggtctggc cacaccgacg
      601 cacggcgtct gccgaccaca acgcttagcg tcaacagtgc cagcaaaacc ggcacggcgt
      661 gcatcaggcc aggcgcatcg ccccagtggt tagccaccaa ggcaacgccg accagcgtgg
      721 cgacaaagct ggcaaaaacc agcggtcctg ctaaagaagt tggctttgtc ggcgggcgca
      781 acgctatcag gccacccagg gtcagcgctg cggtcagtgc ccacactatg taggcacctt
      841 tgggcaaaac caaggcaagc ccggttgcca acaaaatcat aacagcgcca aacatagtcc
      901 ccatcaccca ttggcggcgg ctgaagccac gcatgacctt ggccaatggc aacatcaagg
      961 ccgtggccgt cagcagtgcc gccatgcccc ggtagccact gctttgaaac atccaggcag
     1021 ccccaaatcc aaaagcagca accagcacaa tacccggtag ccatcctgcg ctgtcattgc
     1081 gaccaacagc ttcgtcctgg cggacttgac caccaatcca ggcactcccg gttgagcctt
     1141 gcgtaaccgc tgtcagatca ttgcccgtcg atctttgcgc cgtgctcaac acactgaata
     1201 gcgctactgc gaccggcacc gccaaataga cccctcgtga aaaggttgtc aggcatgcat
     1261 aagccgccaa aaccaatacg gtgacggcca aacccaaacg cgcatggctt cgtgcaatca
     1321 tcaattcacg caaagcaacc ggcacggtca acgccaaaaa accatccaat gcagcgccac
     1381 ccacatgcat ttcccagaac agggccgtgg ttctatagtc tgatgaaaaa tccaatagcc
     1441 cggtaaaagc tgtcctttcc caaaccgtgg caagtgaagc acttgccagc cccagcatga
     1501 tgcccagcga aagcaagcca tgggactgtt caggcttcgt ccgatggttt gccagccaca
     1561 gtggaagcat gatcagtgcc aatagaaaac tcttgccaat gcggatgctg ttcatgggct
     1621 cgtgaaaccc ttggaaccaa ccgaatgaaa aaccacccgc atctgcaaag ccgcgagctg
     1681 tcgctagacc tgttgaaaca acaaacagcg ctatcaatat gtaggaatgc aggggaatac
     1741 tgcggcggct cgcttcaacc ttataaaata ccagacgccc gtaaccgcca acagcggcag
     1801 ccaaaacaag catgtcaagt tcttcaaaag caagccaacc tgtccaaggt gcaaagccaa
     1861 ccaaaggcaa caaccccggt agcgccacaa tccaggcact gggcaataaa aaaaacaggg
     1921 tgccagcagc tccaaaagca acgttcaaca ccggatgggc agccggatag tgcatggtga
     1981 ttgcaaaccc agccataccg ctgccaatcc caaagaatgc actcaacagg cgcataaagt
     2041 atggtttggg aactctggca ttcataaggt ctgatgcgtt ttcttagacg agttagatga
     2101 aattgccgag gatgtggctg ttgtcacgcc aaagacatgc aaagagataa tctggagaag
     2161 aaaggcttcg actgcagtga ttttttcata cttgatcggc aatcaaatta actcgaatgc
     2221 tgatgtttgg ttggttggtt ggttgagatt gctgggtgcc tgtggctgag caatccctgc
     2281 accgtggtcc ttggtggtac cagtttaccg gggagcggca acactccctg acaggagtgt
     2341 cagaatttct ttttgcagca tggataaatg atagcgaagg ttttcggtat ttgaatttgg
     2401 acaagcagcc cgttcaccgc ttacgcttga tacccctcca attacagcga ggcgcaaacg
     2461 cacaacgcca actgcgcaag ataaaccttg ttggactgaa tcgccgctga cagacggtat
     2521 cgataagggg attgaaaaag atcctggtac tttgaggtct tgcagaatta ttttcaaaag
     2581 atggcctgtt aatctttatt gcaactgaat agcaataaat tcgaagtgtc aacgatccat
     2641 tcgtcttgtg cgtcgctaca acatagaaat ccctgccttt tgcatgtgca tcaccctcac
     2701 gctgtttcgt gatcccgttt tagtttggct gattgaccgg acttaaacaa ccatcaagaa
     2761 atactgttaa aaatcaacaa gttagatcgt gctagttttc agttgttaga tttgctcgta
     2821 tttctcgcgt caagccacaa gttttttggt accgcctggc gcttcactga tccccagcgc
     2881 ttggtaaatt ctcatcagcg ctggctcggc cttggtgctc ttgcggatat gaatgctgcg
     2941 tccatcgctg cgctgcatgc tcgctgtgac acgctgttgc accgacaggg tgtttcttaa
     3001 agtggcccaa ctgtcatgaa tgccttgctc cttgcgcgtt ttgcgaatca cctgcacaca
     3061 ttggtacgcc aggacggtca taaacaaatg cccatctgag cgattttcct tttgatgaaa
     3121 cactgggcgc aaacccagtt cacctttcaa gctgcgaaac acactctcga tatccgtcag
     3181 ggttgaatag gtctgccaca gtttttgttc gtcccagtcc agcacgttgg tgcgcaggca
     3241 atacaccccc ggctgcgtgg ccatggtgcc ttggatgaat tcttttttcc agctcagcgt
     3301 aagtgctggt ttatggttct tcgtcgtttt gtgtggaagt tgcattgaaa aataaaatgt
     3361 agtagggct
//
LOCUS       DAOSLL010000542         3357 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24745_length_3357_cov_4.968504, whole genome shotgun sequence.
ACCESSION   DAOSLL010000542 DAOSLL010000000
VERSION     DAOSLL010000542.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24745_length_3357_cov_4.968504"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="PLB25_18525"
     CDS             <1..704
                     /locus_tag="PLB25_18525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43600.1"
                     /translation="AKWLAALAMLILASYLGFWVVLSPERPVPALGSFLFPLAVYAWL
                     GLVLTRQPLAWRLALVAFLTLSLVFDAADNLLPYLYRQSALCQAFRVLPSSDRPETFS
                     CAGESALATYQRLGDDPRKPHSTWIAQAPMTGVHALAGSLIVVDTRSNALRQPEGVAW
                     QLIGLQPHGRGQQIGFQAMAAAGLPPVFVADQGNALSRNNYYVYLHQLSGALQRAGIT
                     EFIIVPATGSNPPLF"
     gene            807..1724
                     /gene="speB"
                     /locus_tag="PLB25_18530"
     CDS             807..1724
                     /gene="speB"
                     /locus_tag="PLB25_18530"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HOE43601.1"
                     /translation="MSISRFAYASNSNRFLAAPELAQQPFAVVGVPFDGAVTNRPGAR
                     FGPQEIRRASLMLCDGIHPYFDVTPLGHLGDGGDMRLPNAAPLADVRQQIQAQASSLM
                     AQHHCVFLGGDHSVTLALLRAAHAIHGALALVHFDAHCDTWTDHFGEPSGHGTWTYEA
                     MQEGLLSAPHTVQIGLRSSGERAARDYVQDQGGLIFNARSLRGQDGSALQPVAAQIRQ
                     RLGKRPCYLTLDIDCLDPAFAPGTGTPEPGGMSSSQVLTLLEELAALNMVGMDCVEVA
                     PAYDHAELTSSAAATFVWTYLCGQIAKPV"
     gene            1738..3039
                     /locus_tag="PLB25_18535"
     CDS             1738..3039
                     /locus_tag="PLB25_18535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOE43602.1"
                     /translation="MLIDSDTQLNQNSYYEASAIRPGPLPPLQQHISADVVVVGAGFA
                     GLSAAIELVRRGYSVVLLEADRVCSGASGRNGGQAIVGYASGQAPFEHQLGRAQARLA
                     WDMSMLSIDLIEARIREFDIDCDWVKGYLHVADSPRKARALQAETEAMARDYGFEADL
                     AIGADVQRHINSPIYHAAAFDTSSGHLHPLKFGLGLARAAQALGVQIHEKSAVVKLDR
                     GSPVLASTAQGSVKAKFCVLAGNCTLSEYGPKVAPEIGARIMPVGTYMIATAPLGAPL
                     CRQLIPSNAAACDNNFILDYFRFSADHRMLFGGRVSYTTRTPTDLRQTMARRMAQVFP
                     QLSQAQVEFLWGGFVDISMNRAPDFGRLGNNVYYLQGFSGHGVALTGLAGQLVAQAIA
                     GQAERFDAFASLQHRKFPGGPLLRMPSLVLGTLYHRLRDAF"
BASE COUNT          561 a         1007 c         1092 g          697 t
ORIGIN      
        1 acgccaagtg gctggctgct ttggcaatgc tgatattggc gagctacctg gggttctggg
       61 tggtcttgtc gccggagcgt ccggtgccag cgctgggttc ctttttgttt ccgctggcgg
      121 tgtacgcatg gctcgggctg gtgctgacgc gccagccgtt ggcgtggcgt ctggcgctgg
      181 ttgcgtttct gacgttgtcg ctggtttttg acgcggctga caacctgctg ccctatttgt
      241 atcgacagtc ggcgctgtgt caggctttcc gggtgctgcc aagctcagac agaccagaga
      301 ctttctcgtg cgcgggtgaa tcggcgctgg cgacttacca gcgactcggt gacgatccac
      361 gcaagccgca cagcacctgg attgctcagg caccaatgac cggtgtccac gcgctggccg
      421 gttcgctgat cgtcgttgac acccgcagca atgcgctgcg ccaacccgag ggtgtggcct
      481 ggcaattgat cggtttgcag ccccacgggc gcggccagca aatcggtttt caggccatgg
      541 ccgccgctgg cttgcccccg gtgtttgtgg cagaccaggg caatgccctg agtcgcaaca
      601 actactatgt ttatctgcac caattgagtg gtgcgctgca gcgcgccggc atcaccgagt
      661 tcatcattgt tccagcgaca ggctccaatc cgccactctt ctgaccagcg cagaaatgtt
      721 acggtggcgt ggcggctgcg ccaacaggaa caggcggtac gcatggcata gactgtgctg
      781 atgcgcaact ttgcagggaa ctcttcatgt cgatcagtcg atttgcctac gccagcaaca
      841 gcaaccgctt tctggccgcg cccgaactgg cacagcagcc gtttgccgtg gtcggcgtgc
      901 cgtttgacgg ggcggtgacg aatcgacctg gggcacgctt tgggccgcag gaaattcgcc
      961 gtgccagcct gatgttgtgt gatggcattc acccgtactt tgacgtgaca ccgctggggc
     1021 atctgggcga tggcggtgac atgcgcctgc ccaatgccgc gccattggct gacgttcgcc
     1081 agcagattca ggcccaggcg agcagcctga tggcgcagca ccattgcgtg tttctgggcg
     1141 gcgaccattc ggtcaccttg gcgctgttgc gcgcagccca tgccatccac ggcgcactgg
     1201 cgctggtgca ttttgatgcc cattgcgaca cctggaccga ccactttggc gaaccctcag
     1261 gccacggcac ctggacttat gaggccatgc aagagggttt gctgagcgcg ccgcacactg
     1321 ttcagattgg gctacgttcc agcggcgagc gcgctgcgcg cgactacgtg caagaccagg
     1381 gtgggctgat cttcaatgcc cgcagcctgc gcggccaaga cggcagcgcc ttgcagccgg
     1441 tggcagcgca aatccgccag cgcctgggca aacggccgtg ctacctgacg cttgacatcg
     1501 actgcctgga cccggccttt gcccccggca ctggcacccc cgagcccggc ggcatgagca
     1561 gttcgcaggt gctcacgctg ctggaagagc tggcagcgct caacatggtc ggcatggact
     1621 gtgtcgaagt cgccccggct tatgaccatg ccgagctgac cagcagcgca gcagccacct
     1681 ttgtctggac ttatctgtgt ggccagattg ccaaacctgt ttgacagaat cgcagccatg
     1741 ttgatcgact ctgacaccca gctcaaccag aacagctatt acgaggccag tgccattcgg
     1801 cccggtccgt tgccaccgct gcagcagcac atcagcgctg atgtggtggt ggtgggggcc
     1861 ggttttgccg gtctgtcggc cgccattgag ctggtccggc gcggctactc ggtggtgctg
     1921 ctggaggctg accgggtttg cagcggtgcc tcggggcgca atggtggcca ggcgattgtg
     1981 ggttacgcca gcggccaggc gccgtttgag caccagttgg gccgggcaca agccaggctg
     2041 gcctgggaca tgtcgatgct gtcaattgac ctgattgagg cgcgcattcg cgagtttgac
     2101 attgactgcg actgggtcaa aggctatttg catgtggctg actcgccgcg caaggcgcgc
     2161 gccttgcaag ctgaaaccga agccatggcg cgtgactacg gctttgaagc cgacctggcc
     2221 atcggggctg acgtgcagcg gcacatcaac agcccgatct accacgcggc agcatttgac
     2281 accagctccg ggcatttgca cccgctcaaa tttggcctgg gtctggcgcg ggcggcacag
     2341 gcgctggggg tgcagattca tgaaaaatct gcagtggtca agctcgaccg gggcagcccg
     2401 gtgctggcca gcaccgcgca gggcagtgtc aaggccaagt tttgcgtgct ggctggcaac
     2461 tgcacgctgt ccgaatacgg cccaaaagtg gcaccggaga ttggcgcacg catcatgccg
     2521 gtgggcacct acatgattgc cacggcgcca ctgggcgcgc cgctgtgtcg gcaactgatc
     2581 ccgagcaatg ccgctgcctg tgacaacaat ttcattctgg actatttccg cttcagtgcc
     2641 gaccaccgca tgctgtttgg cgggcgggtc agttacacca cccgcacccc gaccgatctg
     2701 cgtcagacga tggcccggcg catggcgcag gttttcccgc agttgagcca ggcgcaggtc
     2761 gaatttttat ggggtggctt tgtcgatatc agcatgaacc gtgcccccga ctttgggcgg
     2821 ctggggaaca atgtctatta cctgcagggc ttcagcggcc atggcgtggc gctgaccggg
     2881 ctggccgggc aactggtggc gcaggccatc gccggacagg ccgagcgctt tgatgcgttt
     2941 gccagcctgc agcatcgcaa gtttccgggt gggccgctgc tgcgcatgcc gagcctggtg
     3001 ctgggcacgc tttaccaccg gctaagagat gctttttgag cggttcaggc acagtggcgt
     3061 taagccgggc taaacagatt cggcgggcta tttggtctgg cgcttgatcg aaccggccct
     3121 gctgcctgcc tggccggtgc ggctgccggg ccagatggcg tagcaaccgg gaccaggtgc
     3181 cgggcaagtt tccggctgaa cgatggtggc ttaaagcttg acgaacttgc cgcctttttg
     3241 tgcttcgcgc tgtgtcagcc gggtcagcgc ttgtttgaag ttttgccagc caagcgagag
     3301 tatttcgccc atggcatcag agcgcagttg ctgtgcctga cggatatggg cttgtat
//
LOCUS       DAOSLL010000543         3348 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24811_length_3348_cov_5.096568, whole genome shotgun sequence.
ACCESSION   DAOSLL010000543 DAOSLL010000000
VERSION     DAOSLL010000543.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24811_length_3348_cov_5.096568"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="PLB25_18540"
     CDS             <1..548
                     /locus_tag="PLB25_18540"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HOE43603.1"
                     /translation="TLLIGGGPMMGAPLPSVQAPVVKGSCGVLALTAGEINEGPESPC
                     IRCGKCVSACPAGLVPVEMAQLVRRGDLQAAKAVGVQDCIACGSCSFICPSHIPLAHY
                     FSFANGKLGALDRDARRQDRIRSLVQARSERTAREAEAKKAVAAARKAAKAAAAATQA
                     AVIAVTAEVNATNTSLLEEST"
     gene            545..1633
                     /locus_tag="PLB25_18545"
     CDS             545..1633
                     /locus_tag="PLB25_18545"
                     /inference="COORDINATES: protein motif:HMM:NF015100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HOE43604.1"
                     /translation="MSTLANRAGPFTHTSNSVTRTMLWVVVALAPATGYGLWLFGWPA
                     VLLFVITLASALAAEAVMLALLGQPVRVRLLDGSALLTGWLLALSLPPWAPWWIGFLG
                     SLSAIVIGKHLFGGLGQNMFNPAMVARIVLLISFPVQLTLWVAPAPLGSASAPSLMQS
                     LAYVSGQTALPDHLSAATTLGALKTELSRGVSVVKASAQLAAPMQLAIGDESGSMGES
                     SAVLLLAGGLLLLLLRIITWHIPLALLGTLGLLAAVSNALDPTRFASMPIHLLSGAAM
                     LGAFFIATDYVTSPVSRSGQLLFGAGIGALTWVIRALGGYPEGLAFAVVLMNALVPLI
                     DRGFRPRVFGRSWRGKSLQAKAQPKIHI"
     gene            1630..2298
                     /gene="rsxG"
                     /locus_tag="PLB25_18550"
     CDS             1630..2298
                     /gene="rsxG"
                     /locus_tag="PLB25_18550"
                     /inference="COORDINATES: protein motif:HMM:NF002519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HOE43605.1"
                     /translation="MKPSDHTTSLPASSLTARLGPLLELAGGKHALVLGGFSLVATLL
                     LAVAYGLTKEPIEQSALEDLRHSLEQVIPASIHDNNPAADTARLQINGVPLLVYRARK
                     AGQTTGVAFETSRKGYSGEIRLLMGIDESGKLLGVRVLKHTETPGLGDKIEVSRNDWI
                     TRFTGLSLGNPPEAQWAVKKDGGPFDQFAGATITPRAVVNGIRDGLRLFASHRQALLE
                     DAPK"
     gene            2295..2987
                     /locus_tag="PLB25_18555"
     CDS             2295..2987
                     /locus_tag="PLB25_18555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HOE43606.1"
                     /translation="MSQTKDILVDGVWRNNVVFSQMLSLCPALAVTSAATQGLGMGLA
                     TLAVLIASNALVALLRGLITDEVRIPAFVLIIATLVTIVDLAMNAWLHELHKVLGLFI
                     PLIVVNCLILGRAESFACKNSVALSALDGLAMGAGFTLALTLMGAVREIAGSGTLFSG
                     AAMLLGPAMGALEVRIYPGDGALVMILPAGGFIVLGLMIAAKRTLDARKSAPRRQAAT
                     AANATSPVATAS"
     gene            2984..3256
                     /locus_tag="PLB25_18560"
     CDS             2984..3256
                     /locus_tag="PLB25_18560"
                     /inference="COORDINATES: protein motif:HMM:NF002490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HOE43607.1"
                     /translation="MKVSVTYALADRQAWLEVEVAEQATVRDAIEASGIARTFPALDL
                     STQKVGIFGKVVSLEARLEPGQRVEIYRPITADPKALRAGDDDSDA"
BASE COUNT          558 a          998 c         1119 g          673 t
ORIGIN      
        1 cgacgctgtt gattggtggc gggccaatga tgggtgcgcc actgccgtcg gtacaggcac
       61 cggtggtcaa gggcagttgc ggcgttctgg cgctgacggc tggcgagatc aacgaaggac
      121 ctgagtcgcc ctgcatccgc tgcggcaaat gcgtcagcgc ctgcccggcc ggtctggtgc
      181 cggtggaaat ggcgcaactg gtgcgccggg gcgacctgca ggcggccaag gcggtcgggg
      241 tgcaggactg cattgcctgt ggctcgtgtt cgttcatttg tccgtcgcac attccgctgg
      301 cacattactt cagttttgcc aatggcaagc tcggcgcgct ggaccgcgac gcccgtcggc
      361 aagaccgcat ccgctcgctg gtgcaagccc gcagtgagcg caccgcgcgc gaggccgagg
      421 cgaaaaaggc agtagccgct gcccgcaagg ctgccaaggc ggcagcagca gcaacgcagg
      481 ccgctgtcat tgccgtgacg gcagaagtca acgccaccaa caccagcctg ctggaggagt
      541 caacatgagt accctggcca accgggctgg cccgttcacc cacaccagca acagcgtcac
      601 ccgcaccatg ctgtgggtgg tcgtcgcgct ggcaccggct accggctatg gcttgtggct
      661 gtttggctgg ccagccgtgc tgttgtttgt gatcaccctg gccagcgccc tggcggctga
      721 ggcagtcatg ctggcgctgc tggggcagcc ggtgcgggtt cggctactcg acggctcggc
      781 gctgctgacg ggttggctgc tggcactcag cctgccgccc tgggcaccct ggtggatcgg
      841 ctttctgggc agtctgtcag ccatcgtgat tggcaaacat ttgtttggcg gtttgggtca
      901 aaacatgttc aacccggcca tggtagcgcg tatcgtgcta ttgatttcat tcccggtgca
      961 gttgacgctg tgggtcgcac cagcgccgct gggcagtgcg tcggcaccgt cgttgatgca
     1021 atcgctggcc tatgtcagtg gtcaaactgc gctgccggac cacctgagcg cggccaccac
     1081 gctcggggca ctgaaaaccg agctgtcacg cggggtatcg gtggtcaaag ccagcgcaca
     1141 actggcggca ccgatgcagt tggcaatcgg cgacgaatcc ggctcgatgg gcgaatcttc
     1201 ggccgtgctg ctgctcgcag gtggcctgct gctgctgctg ttgcgcatca tcacctggca
     1261 tattccgctg gctttgttgg gcacgctggg gctacttgct gccgtgtcga atgcgctgga
     1321 cccgacccgg tttgcctcga tgccgattca cctgctgtct ggcgctgcca tgctgggtgc
     1381 gtttttcatt gccaccgact atgtgacttc gccggtatca cgcagtggac agttgctgtt
     1441 tggcgcgggc attggcgcgc tgacctgggt gattcgcgca ctcggtggct acccggaggg
     1501 gctggcgttt gccgtggtgc tgatgaatgc gctggtgccg ctgatcgacc ggggctttcg
     1561 cccgcgcgtg tttggccgca gttggcgtgg caagtctctg caagccaagg cgcaacccaa
     1621 gattcacata tgaagccttc cgatcacact acatcgctgc ctgcaagctc actgacggcc
     1681 cgcctgggtc cgctgctgga actggcaggc ggcaagcatg ccctggtgct gggcggcttt
     1741 tcgctcgtgg ctacgctgtt gctggccgtt gcctacgggc tgaccaagga gccaattgaa
     1801 caaagtgcgc tcgaagacct gcgccattcg ctggaacagg tgattccggc gagcatccac
     1861 gacaacaacc cggcggctga tacggcccgc ttgcaaatca acggggtccc gctgctggtt
     1921 tatcgtgccc gcaaggccgg gcagaccacc ggggtggcgt ttgagacctc ccgcaaaggc
     1981 tacagcggcg agatccgttt gctgatgggt attgacgaaa gcggcaagct gctgggcgtg
     2041 cgggttttga agcacacaga aacccctggc ctgggagaca agattgaagt gagccggaac
     2101 gactggatca cccggtttac cggtttgtcg ctgggtaacc cacccgaggc gcaatgggcc
     2161 gtcaagaaag acggtggccc gttcgatcag tttgccgggg ccaccatcac accgcgtgcg
     2221 gtggtcaacg gcattcgaga cggcttgcga ttgttcgcaa gccaccgcca agccctgctg
     2281 gaggatgcac ccaaatgagt caaaccaaag acatcttggt cgacggcgtg tggcgcaaca
     2341 acgtggtgtt cagccagatg ttgtcgctgt gcccggcgct ggccgtcacc tcggcggcca
     2401 cccagggcct gggcatgggg ctggccaccc tggccgtgct gattgcatcc aatgctctgg
     2461 tggccttgct gcgcggcctg atcaccgacg aggtgcggat tccggccttt gtgctgatca
     2521 ttgccacgct ggtgacgatt gttgacctgg ccatgaatgc ctggctgcat gagttgcaca
     2581 aggtgctggg cctgttcatt ccgctgatcg tggtgaactg cctgattctg ggccgcgctg
     2641 aatcttttgc ctgcaaaaac agcgtggcac tgtcagcgct ggatggactg gcgatgggcg
     2701 ctgggttcac gctggccctg accctgatgg gtgcggtgcg cgaaattgcc ggtagcggta
     2761 cgctgttttc cggtgcggcg atgttgcttg ggccagccat gggggcgctg gaggtgcgga
     2821 tttaccccgg cgatggggcg ctggtgatga tcctgccggc tggcggcttt atcgtgctgg
     2881 ggctgatgat tgccgccaag cgaaccctcg atgcgcgcaa gtcggccccg cgcaggcaag
     2941 cagccacagc ggccaatgcc acctcgccag tggccaccgc atcatgaaag tcagcgtcac
     3001 ctatgccttg gcagaccgcc aggcctggct ggaggtggaa gtggccgaac aagccacggt
     3061 gcgcgatgcg attgaagcca gcggcattgc gcggaccttt ccggcgctcg acctgagcac
     3121 ccagaaggtg ggcatttttg gcaaggtggt ttctttggaa gcgcgcctcg agcccggtca
     3181 acgggttgaa atctaccgcc cgattaccgc agatccgaaa gcactgcgcg caggcgatga
     3241 cgacagcgat gcctgatgcg caactgggct ggttacgctg acggggttca accagggatg
     3301 tggtcagaaa taacttcccc tatttgcacc ggcggatttg ggcgcaca
//
LOCUS       DAOSLL010000544         3345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24843_length_3345_cov_4.618237, whole genome shotgun sequence.
ACCESSION   DAOSLL010000544 DAOSLL010000000
VERSION     DAOSLL010000544.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24843_length_3345_cov_4.618237"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="PLB25_18565"
     CDS             <1..575
                     /locus_tag="PLB25_18565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tripartite tricarboxylate transporter permease"
                     /protein_id="HOE43608.1"
                     /translation="AANNATVTTAMIPLLTLGIPVSNTTAVMLGAFQNYGIQAGPQLF
                     TNSAALVWALIASLYIGNVMLLVLNLPLVGLWVKLLKIPRPRLYAGILIFSTVGAYGM
                     RQSMFDLFVLYGIGVLGLVMRRFSIPSAPVVVAMILGPLAEAQLRNAMSIGEGSAMVF
                     LQRPVSVIMLAVVIGVQILPRILKKRGVVL"
     gene            complement(813..1415)
                     /locus_tag="PLB25_18570"
     CDS             complement(813..1415)
                     /locus_tag="PLB25_18570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HOE43609.1"
                     /translation="MTVRDILKMGDARLLRIAQPVMQFDSDELHLLITDLLDTMRAAD
                     GAGLAAPQIGVDLQVVIFGTNTPNPRYPEAPLVPRTVLINPRIEALPPASSDPVRTSA
                     SGLAQLPTPDLPLEVEDWEGCLSVPGLRGKVPRFARIRYTGFDQFGDAIDRVVEGFHA
                     RVVQHECDHLIGKLYPMRLRDFSQFGFTSVLFPGLDAADD"
     gene            complement(1874..2071)
                     /locus_tag="PLB25_18575"
     CDS             complement(1874..2071)
                     /locus_tag="PLB25_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43610.1"
                     /translation="MRFVYAAAAVAEVNEALAYYKAIDPNLSKRMVREIDDALNLVQQ
                     FPEGWHPLGGGLRQLRLKSFP"
     gene            complement(2074..2283)
                     /locus_tag="PLB25_18580"
     CDS             complement(2074..2283)
                     /locus_tag="PLB25_18580"
                     /inference="COORDINATES: protein motif:HMM:NF021253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HOE43611.1"
                     /translation="MSHTEILETARKLSPVERLHLIDALLESLDEPDPVIEAAWLAEA
                     QDRLTAFERGELPLADLMPVLDQYR"
     gene            complement(2351..>3345)
                     /locus_tag="PLB25_18585"
     CDS             complement(2351..>3345)
                     /locus_tag="PLB25_18585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HOE43612.1"
                     /translation="RRAGDVIPEVVSVVLPAGLSAAADDLPESSADQTLVATPADHQT
                     EPAIERGPVFTMPKTCPVCGSVALREEGEADYRCTGGLFCSAQRKQSILHFAQRRAVE
                     VEGLGDKLVDQLVDSGTIHTLPDLYRLGLTALFNLDRMADKSAQNILAALQKSKQTTL
                     PRFLFGLGIRHVGEATAKEMARHFGKLDAIMDATEPQLLEVADVGPIVAKSLRTFFDQ
                     PHNREVVEQLRACGVTWPEGEPAVRVPTPFDGKTFVLTGTLPTLDREAAKALIEAAGG
                     KVAGSVSKKTSYVVAGAEAGSKLDKALALGLAVLDEAALLALLQAPVANDASGD"
BASE COUNT          695 a          970 c          966 g          714 t
ORIGIN      
        1 aagcggccaa caatgcaacc gtcaccaccg ccatgattcc cttgctgacg ctgggcatcc
       61 cggtgtccaa caccaccgcc gtgatgctgg gagcgtttca aaactacggc attcaggccg
      121 gtccgcagtt gtttaccaat tcggccgcgc tggtctgggc gctgattgcg tcgctctaca
      181 ttggcaacgt gatgctgctg gtgctcaatc ttccgctggt tggcttgtgg gtcaagttgc
      241 tcaagattcc ccgcccgcgg ctttatgccg gcatcctgat cttttcaacg gtgggtgcgt
      301 atggcatgcg ccaaagcatg tttgacctgt tcgtgctcta tggcattggc gtactggggt
      361 tggtaatgcg ccgattcagc attccttcgg caccggtggt ggtggccatg attctggggc
      421 cactggccga agcgcaattg cgcaatgcca tgtcgatcgg tgaaggcagc gccatggttt
      481 tcctgcaacg cccggtttcc gtcatcatgc tggcagttgt gatcggcgtg cagattttgc
      541 cgcgcatact gaaaaaacgc ggggttgtgc tctgaatggt gagctgccta agccggacca
      601 gccgaaaccg aaaaacattc gtcgcgaggg cgcgactcct acacaaacag tcggcgccta
      661 tttgaatttg caggagtcgc gccctcgcga cgaatgactt tgaatgcttg aattcttcgc
      721 atgatttgca aagacttcac ttgttggaga ctgacatcca atcttgccaa gcccgcgcca
      781 cgaacttgcc gcatattcgg cttttccaca cttcagtcgt cggccgcatc caggcccgga
      841 aacagcaccg aggtaaagcc aaactggctg aagtcgcgca ggcgcatcgg gtagagcttg
      901 ccgatcagat ggtcgcattc gtgctgcacc acgcgggcat gaaaaccttc gaccacgcgg
      961 tcaatggcat caccaaactg gtcaaacccg gtgtagcgga tgcgcgcaaa cctgggcacc
     1021 ttgccgcgca ggccgggcac cgacaggcag ccttcccagt cttcgacttc cagcggcagg
     1081 tccggcgtgg gcagttgcgc cagaccagat gcgcttgtgc ggactgggtc gctcgacgcg
     1141 ggtggcaacg cttcgatgcg ggggttgatc aacacggtgc gcggcaccag cggtgcttcg
     1201 gggtagcgcg ggttgggtgt gttggtgcca aaaatcacca cctgcaaatc cacaccgatt
     1261 tgcggcgcgg ccaggccagc gccgtcggcc gcgcgcatgg tgtcgagcag gtcggtgatc
     1321 agcaggtgca gttcatccga gtcgaactgc atcaccggct gggcaatgcg caacaggcgc
     1381 gcatcaccca tcttgagaat gtctcggact gtcatgggaa agtcaaaagg tgttcaggcg
     1441 gtcgcgccag tagccggggc gacggtgggt gttggcaaag gcgacgatgg cgttcccgtc
     1501 gttatcagtg gtgtagacaa gaacataagg aaacgatttg agtctaaccg gggtttaacg
     1561 taagtttttg caaggtctaa taatttcgac atcatcgccg ctattaatgt aactttagac
     1621 actgttattg atatttagca gcttttagaa acatcgagta acaaaattcg ttttgttgtg
     1681 ccaaaaaatt ggggttgtgc cataaagcca tttgtggttt tcagctagca tggaatcttc
     1741 acgttattaa caagccagca ccaagaaaat gattatttga catgaaatag cgagcaaatt
     1801 cgacgaacaa aatactagta cgcgctaagt cgttgatttg attcagtgtt tttcctgctc
     1861 tgttaaagac cggctaagga aacgatttga gtcgcagttg gcgcaagccg ccacccaatg
     1921 gatgccaccc ttcgggaaat tgctggacaa gattgagcgc gtcgtcaatt tcccgcacca
     1981 ttcgcttgct caaattcgga tcaatggcct tgtaataggc cagcgcctca ttcacttcag
     2041 cgaccgcagc ggcggcataa acaaaccgca tggttaacgg tactgatcaa gcactggcat
     2101 cagatccgcc aaaggcaact caccccgctc aaatgccgtg agccggtctt gcgcctcggc
     2161 cagccaggcg gcttcaatga ccgggtctgg ctcgtcgagc gactccagca gggcatcaat
     2221 cagatgcagc cgctctactg gactcagctt gcgggcagtt tcgaggattt cggtgtggga
     2281 catggctgca ttttgcacag aaaacgggtc gttcgcaaac aatgctggat cgaaccagaa
     2341 ccggcagcaa tcagtcacct gacgcatcgt tggcaaccgg cgcctgcagc agcgccagca
     2401 gggcggcttc atccagcacc gccagcccca gcgccagcgc cttgtcgagc ttgctgcccg
     2461 cctcggcccc ggccaccaca taactggttt ttttgctgac tgagcctgcc accttgccgc
     2521 ccgccgcctc gatcaatgcc ttggcggctt cgcggtccag cgtgggcagg gtgccggtca
     2581 gcacaaaggt cttgccatca aagggtgtgg gcacccgtac agcaggttcg ccctctggcc
     2641 aggtgactcc gcaagcacgc aactgctcaa ccacctcgcg gttatgcggc tggtcaaaaa
     2701 aggtgcgcag acttttggcc acgatcgggc caacatcagc cacttcaagc aactgcggct
     2761 cagttgcgtc catgatcgca tcgagcttgc caaagtgccg cgccatttcc ttggcggtgg
     2821 cctcgccgac atgccggatg cccagcccaa acaggaagcg cggcaacgtg gtctgctttg
     2881 acttttgcag cgctgcaagg atgttttgcg ccgacttgtc tgccatgcgg tccagattga
     2941 acagcgctgt gaggcccagc cggtacaggt ctggcaaggt gtgaatggtg ccgctatcga
     3001 ccagttggtc aaccagcttg tcgcccaggc cttccacctc gaccgcacgg cgctgggcaa
     3061 aatgcaggat cgactgtttg cgctgggcac tgcaaaacag cccgccagtg cagcggtagt
     3121 cggcctcgcc ctcttcgcgc agcgccacgc tgccacacac cgggcaggtc ttgggcatgg
     3181 tgaacaccgg gccgcgttca attgctggct cggtctggtg atcagcaggt gttgctacca
     3241 gtgtctggtc ggccgacgat tccggcaggt cgtccgccgc tgcggacaaa ccggcgggca
     3301 atacgacact gaccacctcg ggaatcacat cgccggcgcg gcgca
//
LOCUS       DAOSLL010000545         3340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24887_length_3340_cov_4.495586, whole genome shotgun sequence.
ACCESSION   DAOSLL010000545 DAOSLL010000000
VERSION     DAOSLL010000545.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24887_length_3340_cov_4.495586"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            163..1671
                     /locus_tag="PLB25_18590"
     CDS             163..1671
                     /locus_tag="PLB25_18590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HOE43613.1"
                     /translation="MQNGTRLAAVDLGSNSFRLEIGRVEGGQFYRTEYLKETVRQGGG
                     LDEARNLTPAAMQSGWDCLARFGERLAGFSTDEVRAVATQTLREARNRDEFLRPGGQL
                     LGFPIDVISGREEARLIYQGVAQALPRKEQRRLVIDIGGRSTELILGSGLEPMQMESY
                     RVGSIAWSMRYFTNGQFAKRSFQTAEIAAKAVLDEAISLYQPDQWEVAYGSAGTVNAV
                     GDVLVAAGWPTGSISQGGLDWLLDKLIAAQSADRLRLPGMKEDRKAIIGGGLSILRAL
                     FDLLGIENLLLTSGGLRHGLLMDMLGQNQHHSDLRTHSVERLAAKFGADPVHGKRVGH
                     VASVLLERLCANMPQSDAGALARMQEKLLWAGQLHEIGSHISHSDYHKHGAYILDNTD
                     AMGFALTELHQLSLLVLGHRGKLRKLDVDFCDEVFVQKLLVLRLAVVLCHARNDPDLQ
                     GLALSRDSKIANRFVLVCPKGWVLAFPQSAHLLNEESVAWQKTPWSLTVQGC"
     gene            complement(1659..2018)
                     /locus_tag="PLB25_18595"
     CDS             complement(1659..2018)
                     /locus_tag="PLB25_18595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43614.1"
                     /translation="MNWLAKLPGFQRTPYGFELRLLRLLPNLLLAGTVLPVLMAALGR
                     VLITQGTAVEIERQIQTFDFVMTGVVIFVWTAVLTVAIGCVIVWLMKGPAYVADAYEV
                     SHSDLPKKNHNSIDQQP"
     gene            complement(2376..2879)
                     /locus_tag="PLB25_18600"
     CDS             complement(2376..2879)
                     /locus_tag="PLB25_18600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE43615.1"
                     /translation="MNDPVSVRLCQVDYSDPEQVGALITLLDAYARDPAGGGQPLTDF
                     VKSGLPAALLARPFMFSVLAFDDAQNSLPVGLVNCVEGFSTFACKPLVNVHDVVVAAS
                     HRGRGIAEQMLALVRQIALARGACKLTLEVLAGNLPAVKLYRRIGFANYQLDPSLGDA
                     CFMQQWL"
     gene            complement(2890..>3340)
                     /gene="glmM"
                     /locus_tag="PLB25_18605"
     CDS             complement(2890..>3340)
                     /gene="glmM"
                     /locus_tag="PLB25_18605"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464306.1"
                     /note="catalyzes the conversion of glucosamine-6-phosphate
                     to glucosamine-1-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HOE43616.1"
                     /translation="KARGVELVRAQVGDRYVLEEMEKHHWLLGGEGSGHLLALDKHTT
                     GDGLISALQVLQTVVRSGKRLSELLNNVTLFPQTLINVRLKPGQNWQASPDLEGIIRA
                     VEGELGQTGRVLIRASGTEPLVRVMVEARDAAQAKNCAQRIAKTLAD"
BASE COUNT          711 a          991 c          999 g          639 t
ORIGIN      
        1 cccacaggac atggcacagg cggtgcatgc catcgggcag gcgctgcagg acattgggct
       61 ggcggcgaat gattgaacct gctgattttt ggtgccaatg ccgccaatcg gtgaacctgg
      121 caatgctatg ctgacacctg ttagccaaga atgatgaaca acatgcaaaa cggaacacgc
      181 ctggcagcgg tagatttggg ctcgaacagc tttcgccttg aaattggcag ggttgaaggt
      241 ggccagtttt accgcaccga atacctgaaa gaaaccgtcc gccagggcgg cggcctcgac
      301 gaagcccgca atctgacacc ggctgcgatg caaagtggct gggattgtct ggcgcgcttt
      361 ggtgaacgcc tggccgggtt ttcgacagac gaagtgcgcg cagtcgcaac ccagaccctg
      421 cgcgaagcgc gcaaccgcga cgagtttttg cgccctggcg ggcaactgct tgggttcccc
      481 atcgatgtga tctcgggacg cgaagaggcg cggttgattt accagggtgt ggcacaggca
      541 ctgccacgaa aagagcaacg tcggctggtg attgacattg gcggacgctc gaccgagctg
      601 atattgggca gtggactcga acccatgcaa atggagtctt accgggtcgg cagcattgcc
      661 tggtcaatgc gctatttcac caatgggcag tttgccaaac ggtcttttca aactgctgaa
      721 attgccgcca aggccgtgct tgatgaagcc atctcgctgt accagccaga ccagtgggaa
      781 gtggcctacg ggtcggctgg tacggtcaat gcggtcgggg atgtgctggt ggcagcagga
      841 tggccgaccg gctcgatcag ccaggggggg ctcgactggc tgttggacaa actgattgca
      901 gcacaaagcg ccgaccggct gcgcctgccc ggcatgaaag aagaccgcaa ggccatcatt
      961 ggcggtggct tgagcatttt gcgggccctg tttgacctgt tgggcatcga gaacctgttg
     1021 ctgacctcgg gtggcctgcg ccatggcttg ctgatggaca tgctgggtca gaaccagcac
     1081 cacagcgact tgcgcaccca ttcggtggaa cgtctggccg ccaaatttgg cgccgacccg
     1141 gtgcatggca agcgggttgg acacgtggcc agtgttttgc ttgagcgcct ctgcgccaat
     1201 atgccccaaa gtgatgccgg cgcgttggcg cggatgcagg aaaaactgct ttgggccggc
     1261 caactgcatg aaattggcag tcacatctcg cacagcgatt accacaagca tggagcctat
     1321 attctcgata acaccgatgc catgggcttt gccctgaccg agttgcatca gcttagcttg
     1381 ctggtacttg ggcaccgggg caagctgcgc aagctcgatg tggatttctg cgacgaggtt
     1441 ttcgtgcaga agttgctggt gttgcggctg gccgtggttt tgtgccacgc ccgcaacgac
     1501 cccgatctgc aaggccttgc gctgagccgg gacagcaaga tagctaaccg ctttgtgctg
     1561 gtttgcccca agggctgggt actggctttt ccgcaatctg cccatctgtt aaacgaagag
     1621 tcagtggcct ggcaaaaaac gccttggtca ctgaccgttc agggctgctg atcgatcgag
     1681 ttgtgatttt ttttgggcag gtcgctgtgc gaaacctcat aagcatcggc aacataggcc
     1741 gggcccttca tgagccagac gatcacgcag ccaatggcca ccgtcaacac tgccgtccag
     1801 acaaagatga caacgccggt catcacaaaa tcgaaggtct gaatctgtcg ctcgatctcg
     1861 actgccgtgc cctgggtgat cagcacgcgc cccagcgccg ccatcagaac cggtaacacc
     1921 gtaccggcca gcaacaggtt gggcagcagg cgcaacagac gcaattcaaa cccataaggt
     1981 gtgcgctgaa agccgggcaa tttggcaagc cagttcatcc tggattcctc gccaagacgc
     2041 gcccgagtgg gccgctggca gcgctcctgg gcagggcgaa agccgcagcg ccagacacgc
     2101 ctgggcaatt aggggtgact tgaagcaggc cagccttgtg acctggctgc gccttgcatg
     2161 ccgcagcagg ctgcgcgggg cgacgacgct ggctgttgct cgacatgagg cgcaacgacc
     2221 ccaggcacgt tgcctgcggt gcaatcgggc ttgccgccct gtcacttgat cggcgaaccc
     2281 agccggagct gaaaaagtca ctgttcatga cgactttcag gcgaaatcaa gctgtcaacc
     2341 gagcaacctg ggttcatgtg ttcatccggg tttgatcaaa gccactgctg catgaagcag
     2401 gcatcgccca gagacgggtc caactggtag ttggcaaaac caattcgccg gtacagcttg
     2461 accgctggca ggttgcccgc cagcacttca agtgtcagct tgcaagcacc acgcgccagc
     2521 gcaatctggc gcaccagcgc gagcatctgc tcggcgatgc cccggccacg atgtgaggcg
     2581 gcaaccacca catcatgcac attcaccagc ggcttgcagg caaaggtaga aaacccctcg
     2641 acacagttga ccaacccgac cggaagggaa ttctgagcgt cgtcgaaagc cagcacactg
     2701 aacatgaagg gacgcgccag cagcgcggca ggcaagccac tcttgacaaa atccgtcagg
     2761 ggttggccgc cgccagccgg gtcccgggca taggcatcca gcagcgtaat caacgcgcca
     2821 acctgctctg ggtcagagta gtccacctga cacaatcgca ctgaaaccgg atcattcatg
     2881 caagctccgc tagtcggcca gggttttggc aatgcgctgc gcgcagtttt tggcctgcgc
     2941 agcgtctctt gcttcaacca tcacgcgaac cagcggctcg gtgccgctgg cccgaatcaa
     3001 aacccgcccg gtctgaccca attcgccctc aactgctcgg atgatgccct ccaggtcagg
     3061 gctggcctgc cagttttggc caggcttgag ccgcacattg atcagggtct ggggaaacaa
     3121 ggtgacattg ttgagcaact ctgacagccg cttgccactg cgaaccaccg tctgaagcac
     3181 ctgcaaggcc gaaatcaagc cgtcaccggt ggtgtgcttg tccagcgcca gcaagtggcc
     3241 agagccttcg ccgcccagta gccaatggtg tttttccatt tcctcaagca cataacggtc
     3301 accaacctgg gcccgcacca gttcaacccc gcgcgccttc
//
LOCUS       DAOSLL010000546         3340 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24888_length_3340_cov_4.105632, whole genome shotgun sequence.
ACCESSION   DAOSLL010000546 DAOSLL010000000
VERSION     DAOSLL010000546.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24888_length_3340_cov_4.105632"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="PLB25_18610"
     CDS             <1..371
                     /locus_tag="PLB25_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43617.1"
                     /translation="NVMLLERTGQYHLAASEIKALFRDDVVDYDLVYAGYLVGARTHD
                     WALMIQALELRIDRWPREAVDGWLKLGEIYSQKMEVKDHNKALIAYRTALFATPESAK
                     EATLSKIPQDYRLKLSGVGE"
     gene            complement(434..886)
                     /locus_tag="PLB25_18615"
     CDS             complement(434..886)
                     /locus_tag="PLB25_18615"
                     /inference="COORDINATES: protein motif:HMM:NF024751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOE43618.1"
                     /translation="MGENFPDLASHCHGHFINAVFQSEGIVPKNWRVGLRWQPNWLYL
                     PGEIGCQFVQLAGIDGAGWHSSKKFLTPPNNRLLALPPYAPELNPVEHLWDELRVNCF
                     HNKVFASLGALEDDLAMGLLAMENTPEVVQSIANWSWTIDVLSKWKEN"
     gene            899..1327
                     /locus_tag="PLB25_18620"
     CDS             899..1327
                     /locus_tag="PLB25_18620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009520132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOE43619.1"
                     /translation="MLVGTSLICSAQQEPVGAVKTVSGEAWVSTAGQRTKAQIGTVVY
                     LGSLLKTARAASMGVTFRDNTLMSFGPDTELTVDEYLFAPTEGKLKLGARLDKGSLNY
                     VSGVIAKLKPDAVNVKTPTGTIGVRGTQFLVKVEAPAAVN"
     gene            1394..>3340
                     /locus_tag="PLB25_18625"
     CDS             1394..>3340
                     /locus_tag="PLB25_18625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HOE43620.1"
                     /translation="MYLRTRLTALALALMVSYAGQAQVLPEPLLQAARKAVLTNPEVQ
                     ARWNGFKAANNEQDALRGGFLPQIDLASSMGRENRVTPIADYGKYSFRTANITLNQML
                     FDGRFTAHEVKRLGFVRLGRYYELAEVSEAIALEAVLAYADVVRYRELVQVGTDNYAE
                     HKQSALKLEEGAKSGLRRGADAEQANGRLALAEASLVTELSNLHNASARYLRVIGESP
                     PTVLPTLPDQFKLGTMPVSVDALLRDGLQGNPTLNAALENMRASQQSIASRKAAFLPR
                     LDLNASASRDKNTDGITGRTRVEGIGLALNGNLYRGGSDNARKNQATDQKNQARERQD
                     STCRDVRQTLSVAFSNVRALTEQQKYKDQQRLATEKSREAYRQQFDIGQRTLLDLLNS
                     QTEFFDATRAYINAHYDQIGAQARTLAGMGRLVATLGADRTDVPAAKDVGQDRTGIDP
                     SDLCPPDETEVDTVDAIKARLAPAKGPGSYVMLIPSPDGTIGRVMVQGKDGQQTLTQA
                     GQVVSMDGGAMPVEVVVSKQQLDRDFKAAIAARPPIPEQFVLYFARGGTQLTKASRAL
                     LPDLLARARTRKTLDIWLVGHTDTVGTNKRNDALGLQRAKSIAKLFQQKGLKDLVITM
                     ESYGERSPQVATPDETDEPRNRRGE"
BASE COUNT          798 a          914 c          948 g          680 t
ORIGIN      
        1 tcaatgtgat gcttttggag cgtacaggcc aatatcatct ggctgcaagt gaaatcaagg
       61 cacttttccg agatgacgtg gtggactatg acttggtgta tgccggatat ctggtcggcg
      121 ctcgaaccca tgactgggca ttgatgatcc aggcactgga actgcgcata gaccgatggc
      181 caagagaagc ggtggatgga tggctgaaac ttggtgagat atacagccaa aaaatggaag
      241 tcaaagacca taacaaagcc ctgatagctt accgaacagc gttattcgcc acacctgagt
      301 ctgcgaagga agccaccctt agcaagatcc cgcaggatta ccggctgaag ctgtccggag
      361 ttggagaata gaaccggtca atcagacgat aggagacttg caagaacccc gagggctcgt
      421 tgagccgctg gcgctaattc tctttccact tcgataagac atcaatagtc caagaccagt
      481 tggcaattga ctgaacaact tcgggtgtgt tttccatggc caacagcccc attgccaaat
      541 catcttcaag tgcaccaagg ctagcgaaga ctttgttgtg aaagcaattc acgcgcagtt
      601 catcccagag atgctcaact ggattgagct caggagcgta gggcggcaat gcgagtagcc
      661 ggttgtttgg cggtgtcagg aattttttgc tggagtgcca tccggctccg tcgatcccgg
      721 ccaactgcac gaattgacag ccaatttcac caggaaggta tagccaattt ggctgccagc
      781 gcaagccaac ccgccagttt tttggcacaa tgccctcgga ttggaaaact gcattgatga
      841 aatggccatg acaatgactt gcaagatctg gaaaattttc ccccaatgcg tgggtctcat
      901 gcttgtcggc acttccctga tttgctcggc tcaacaagaa ccggtcggtg ctgtcaaaac
      961 agtgagcggc gaggcctggg tttcaaccgc agggcaacgc accaaggcgc agattggcac
     1021 cgttgtctat ctgggtagcc tgctaaagac cgcccgggct gcatccatgg gtgtaacttt
     1081 cagggacaac accctgatgt cgtttggccc tgatactgaa ttgaccgtcg acgagtacct
     1141 tttcgcccct actgagggga agctaaaact cggtgcccgg ttggacaaag gcagcctgaa
     1201 ttacgtctcg ggagtcattg ccaagctcaa acctgatgcg gtgaacgtca agaccccaac
     1261 cggcaccatt ggtgtgcgcg gtacccagtt tcttgtcaag gtcgaagcac cggcagccgt
     1321 caattagcgc attagaacga cagtatccaa cggcgttgcc gactgccagt tctttatttt
     1381 ttcagggtcc ctcatgtact taagaaccag actgacagct ttggccttgg cgctcatggt
     1441 cagttatgcg ggtcaagcac aggtgcttcc cgagccattg ttgcaagctg cccgcaaggc
     1501 cgtgttaacc aaccctgaag tgcaagcccg ctggaacggc tttaaggctg ccaacaatga
     1561 gcaggatgcg ctgcgcggtg ggtttctgcc ccaaatcgac ctagccagct caatgggccg
     1621 agaaaaccgc gtgacaccga tcgcggacta cggcaaatac agttttcgca cggcaaatat
     1681 caccctgaac cagatgttgt ttgatggcag gttcaccgcc catgaggtta aacgcctggg
     1741 atttgtaagg ttggggcgct attacgaatt ggctgaagtt tcagaagcta ttgccctgga
     1801 ggcggtgctg gcctatgcag atgtggttcg ctatcgtgag ctggtgcagg ttggtaccga
     1861 caattatgcc gagcacaagc agtccgccct gaagcttgag gaaggtgcca agtccggcct
     1921 gaggcgcggt gccgatgcgg aacaggccaa cggtcgcctg gcactggcag aggccagtct
     1981 ggtgaccgaa ctgagcaact tgcacaacgc aagcgcgcgt tatctgcgcg tgattggaga
     2041 atctccgccg accgtcttgc caacactgcc tgatcagttc aagctgggaa ccatgccggt
     2101 gtcagttgat gcgcttttgc gtgatggcct gcagggcaac ccgacgctca atgcagcatt
     2161 ggagaacatg cgcgccagcc agcagtccat tgcgtcacgc aaggcagcgt ttctgccgcg
     2221 tctcgacctc aatgcatccg ccagccggga caagaacacc gacggtataa caggaaggac
     2281 ccgtgtcgag ggtatcgggt tggcattgaa tggcaacctt tacaggggtg gttccgacaa
     2341 tgcgcgcaag aaccaggcaa cagatcagaa aaaccaggcg cgcgagcgtc aggacagcac
     2401 ctgccgcgat gtgcgccaaa ccttgtcagt cgcgtttagc aacgtccggg ccttgaccga
     2461 gcagcagaaa tacaaggatc agcagcgtct tgcgacggaa aaatcacgcg aagcctaccg
     2521 ccagcagttt gacatcggcc agcgcacgct gcttgacctg ctgaacagcc agacagagtt
     2581 ctttgacgca actcgtgcct acatcaacgc gcactacgac cagattggtg cacaggcgcg
     2641 cactttggct ggcatgggca ggttggttgc cacccttggt gccgaccgca ccgatgttcc
     2701 agccgcgaag gatgtcggcc aggatcgcac gggcattgat ccgtctgacc tgtgtccacc
     2761 cgacgagact gaagttgata ccgtggatgc gatcaaggcc agactagccc ctgccaaagg
     2821 tccaggctct tacgtgatgc tgatccctag tccagacgga actatcggcc gggtgatggt
     2881 gcaaggcaag gacggccagc aaactttgac ccaagccggc caggtggtgt cgatggatgg
     2941 tggtgcaatg cccgttgaag tggtggtgag caaacagcaa ctcgaccgcg atttcaaggc
     3001 agccattgca gcacgcccgc cgataccaga gcagtttgta ctgtactttg cgcgaggcgg
     3061 cacacagctt accaaagcat caagggcgct gctacctgac cttctggcca gagcccgcac
     3121 gcgaaagacg ctcgatatct ggctggtcgg ccacaccgac acggttggta caaacaagcg
     3181 caacgatgct ttaggccttc agcgcgcaaa gtccatcgcc aagctttttc agcaaaaggg
     3241 cctgaaggac ctggtcataa cgatggagtc ctatggtgaa cgcagcccgc aggttgccac
     3301 acctgacgaa actgacgagc cgcgcaaccg ccgtggcgaa
//
LOCUS       DAOSLL010000547         3337 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24921_length_3337_cov_4.431140, whole genome shotgun sequence.
ACCESSION   DAOSLL010000547 DAOSLL010000000
VERSION     DAOSLL010000547.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24921_length_3337_cov_4.431140"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="PLB25_18630"
     CDS             complement(<1..498)
                     /locus_tag="PLB25_18630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011831020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOE43621.1"
                     /translation="MARHTPIERYRNIGISAHIDAGKTTTTERVLFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTCFWKGMDKSLPEHRINIIDTPGHVDFTIEVERS
                     MRVLDGACMVYCAVGGVQPQSETVWRQANKYKVPRLAFVNKMDRTGANFFKVVEQMKL
                     RLKASP"
     gene            complement(597..1070)
                     /gene="rpsG"
                     /locus_tag="PLB25_18635"
     CDS             complement(597..1070)
                     /gene="rpsG"
                     /locus_tag="PLB25_18635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008649176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HOE43622.1"
                     /translation="MPRRREVPKREILPDPKYGNIELSKFMNVIMEGGKKAVAERIIY
                     GALEQIAKKHPDKDAMEAFTVAINNVKPLVEVKSRRVGGANYQVPVEVRPVRRLALSM
                     RWIKEAARKRGEKSMALRLANELLEATEGRGGAMKKRDEVHRMAEANKAFSHFRF"
     gene            complement(1218..1595)
                     /gene="rpsL"
                     /locus_tag="PLB25_18640"
     CDS             complement(1218..1595)
                     /gene="rpsL"
                     /locus_tag="PLB25_18640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HOE43623.1"
                     /translation="MPTINQLIRQGRAVETVKSKSPAMENSPQRRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNGFEVISYIGGEGHNLQEHSVVLVRGGRVKDLPGVRYHIVRGSL
                     DLQGVKDRKQSRSKYGAKRPKAK"
     gene            complement(1728..2873)
                     /locus_tag="PLB25_18645"
     CDS             complement(1728..2873)
                     /locus_tag="PLB25_18645"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HOE43624.1"
                     /translation="MKKILLAWFAVACFSAAAQAPQAPEIAARSYLLLDVTADQILAQ
                     KDIDAPVEPASLTKLMTAYLVFDALRSKKIDLKQTFPVSERAWKMPGSRMFIDPKMNV
                     PVEDLIKGMIVQSGNDATIALAEGVGGSTERFVQLMNDQARVLGLKATMYKNPEGLTE
                     PGHTTTARDLSVLATRLMRDFPDYVAYYAIKKYRYIGTPSANDSNRNLLLFRDPTVDG
                     LKTGHTEAAGYCLIATAHRDVPGVGSRRLLSVVLGAASENIRASESQKMLNWGYTAFE
                     AVKLFDANQAVVTPTVWKGKEAKVALGQTRAIVVAVPVGTGSKLSTEVVRAEPLVAPF
                     VKGQQIGLLKISGSGQVVAEVPLVALQGVEQAGVLGRAWDAMRLWIK"
     gene            complement(3088..>3337)
                     /locus_tag="PLB25_18650"
     CDS             complement(3088..>3337)
                     /locus_tag="PLB25_18650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HOE43625.1"
                     /translation="ATTAFERCKSLVKAAGLAGPGEVRVNKAGCLDRCTAGPVAVVYP
                     EGVWYSYVDDADIDEIVESHLKNGHVVQRLLTPTHLGR"
BASE COUNT          772 a          956 c          843 g          766 t
ORIGIN      
        1 gggactggcc ttcaggcgca atttcatctg ctctacaacc ttgaagaagt tggcaccggt
       61 gcggtccatc ttgttcacaa aggccagacg tggcaccttg tacttgttcg cctgccgcca
      121 aaccgtctcg gattgaggct gcacaccacc aacggcgcaa tagaccatgc aggcaccgtc
      181 tagcacacgc atggagcgct caacttcaat ggtgaagtca acgtgaccag gggtatcaat
      241 gatattgatc cgatgctcgg gcaaggattt gtccataccc ttccaaaaac aggttgtggc
      301 agcagatgtg atcgtaatac cacgctcttg ctcctgctcc atccagtcca tggtggccgc
      361 gccgtcgtgc acttcaccga tcttgtggtt cacaccagta tagaaaagca cgcgctcagt
      421 ggtggtggtt ttaccagcgt caatatgggc tgaaatgcct atattgcgat agcgctcaat
      481 gggggtatgg cgagccatta tggatcctta ggtgatcggt atcttgaaag tcaacaagcc
      541 gcagtacgcg acttggcacg cagatctcag actatctgag atctgcgtca aaagtattaa
      601 aaccggaagt gtgagaaagc tttgttggct tcagccatgc gatgaacctc gtcacgcttt
      661 ttcatggcac caccacggcc ttcggtggct tcgagcaatt cgttggccaa gcgcaacgcc
      721 atcgattttt ctccgcgctt gcgggcggct tccttgatcc agcgcatcga cagggccaag
      781 cggcgcactg ggcgcacttc aactggcacc tgatagttgg caccaccaac acgacgggat
      841 ttgacttcaa ccagcggctt gacgttgttg atggcaacgg taaaggcttc catcgcatct
      901 ttatcggggt gcttcttggc gatttgctca agggcaccat aaatgatacg ttcggctacc
      961 gccttcttgc cgccttccat gatcacattc atgaacttag acagctcgat attgccgtac
     1021 tttgggtcag gcaggatttc acgtttggga acttcgcgac gacgtggcat ttctttacct
     1081 cttttgcttc agttggtgtt tccaacaaca ccgcgagagt taaaaataag actctcactt
     1141 actcgacccg aacattgggc cactgcgttc atccattgcg ccacgcaaca aacaaacacc
     1201 gaaaaaaaac gactagctta cttggctttg ggccgcttgg caccatattt ggagcgagac
     1261 tgcttgcggt ctttcacacc ttgcaagtca agcgagccac gcacaatgtg gtagcgcaca
     1321 ccgggcaagt ccttgacccg accaccacga accagcacca cgctgtgctc ctgcagattg
     1381 tggccttcgc caccaatgta agaaatcacc tcgaacccgt tggtcaagcg aactttggca
     1441 actttacgca aagccgagtt gggctttttc ggggtagtgg tgtacacgcg ggtgcacact
     1501 ccgcgacgct gtggagaatt ttccatcgcc gggcttttgg acttgacggt ctccacagca
     1561 cgcccctgac ggatcaactg attgatggtt ggcattgagc ctcctaatga ctggacatcg
     1621 caaaatgccc cttatcgcta aaaaaacttg aattagacaa aaacgtgaac cccttcggaa
     1681 tttccgaaaa gccctctact atatcaggca aaggcttatc cgacaattca tttgatccac
     1741 aagcgcatcg catcccaggc gcgacccaac actccagcct gttccactcc ttgcagcgca
     1801 actagcggca cttcggcgac gacctgcccg gagccactta ttttgagcag gccgatttgt
     1861 tgtcccttta caaaaggtgc caccagcggc tcggcgcgaa cgacctcagt actcagtttg
     1921 ctgccggttc caacgggcac ggccaccaca atcgcccgcg tttgacccaa ggcgactttg
     1981 gcttccttgc ccttccagac ggtgggggtg acaaccgctt ggttggcatc aaacaacttt
     2041 accgcctcaa aagcggtgta accccagttc agcatctttt gcgattcact ggcacggatg
     2101 ttttcactgg cggcccccag cacgaccgat aagagacggc gactgcccac ccctggcaca
     2161 tcgcggtgcg ccgtggcgat cagacaatag ccagccgcct cggtgtgtcc ggttttcaaa
     2221 ccgtcaaccg ttgggtcacg aaaaagcaac agattacggt tgctgtcatt ggcagacggc
     2281 gtgccgatat agcggtattt cttgatggca tagtaggcca catagtctgg aaagtcacgc
     2341 atcaagcgcg tcgccaacac gctgaggtcg cgcgccgtgg tggtgtgtcc tggctcagtc
     2401 agaccctccg gattcttgta catggtcgct ttcaggccca gcacccgggc ctgatcgttc
     2461 atcagttgca caaatcgctc agtgctacca cccacgcctt cagcaagcgc aatcgttgca
     2521 tcgttgccac tttgcacgat catgcccttg atcaggtctt ccaccggcac attcattttg
     2581 ggatcaatga acatgcgcga acctggcatt ttccaagcgc gctcactcac cggaaaagtc
     2641 tgtttgaggt caattttctt gctgcgcagg gcatcaaaca ccaggtaggc cgtcatcagc
     2701 ttggtcagcg atgcaggctc tactggcgca tcaatgtctt tttgcgccag gatttgatcc
     2761 gcagtcacat cgagcagcaa ataagaacga gccgcaattt ctggggcttg aggtgcctgg
     2821 gctgctgcag aaaaacacgc gacagcgaac caggccaata agattttttt catatgcatt
     2881 ggtttgccca ctggaaacca gcaagcatca gaagttgata taaatgttat agctaccagc
     2941 gcttgataga taagggctag agcacaaaaa ggttcaataa taagagtgac gcaaaattgc
     3001 tctggcgata cacgttgcca caggaagcaa cggttctatg gcaacgcaac gatgcagagg
     3061 ctcttttgcg caagtcctga tcagaaacta tcgtcccaga tgggtcggtg ttagcaggcg
     3121 ctgcaccaca tggccgtttt ttaggtgcga ctcgacgatc tcgtcaatgt cggcatcgtc
     3181 cacgtagcta taccataccc cctcaggata caccacggcg accggtccgg cggtgcagcg
     3241 gtccaggcat ccggccttgt taacgcgaac ctcccccggg cctgccagcc cagcagcctt
     3301 aaccagggac ttgcagcgct caaaggcggt ggtggcg
//
LOCUS       DAOSLL010000548         3335 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24945_length_3335_cov_5.045122, whole genome shotgun sequence.
ACCESSION   DAOSLL010000548 DAOSLL010000000
VERSION     DAOSLL010000548.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24945_length_3335_cov_5.045122"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="PLB25_18655"
     CDS             complement(<1..310)
                     /locus_tag="PLB25_18655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007832740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE43626.1"
                     /translation="MITDFKHKTAVLTGAGSGFGLEMARLGARLGMNLVLVDVQQDAL
                     DAAQAELVAAGAEVLAVRLDVSQAAEVEALGRVVFERFGAPHLVFNNAGVFASCLTWE
                     A"
     gene            complement(468..959)
                     /locus_tag="PLB25_18660"
     CDS             complement(468..959)
                     /locus_tag="PLB25_18660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE43627.1"
                     /translation="MTFKLWSIGGGTRSNALVWRLLAFDDLRVSELYAVLRLRSEVFV
                     VEQQCVFHDMDGYDPQAMHLLGVQHGQLQAYARCFEAGIKFPEASFGRVLVRQSARGT
                     GLGHTLIEQTVTAISQLWGPRPIRIGAQLQLAAFYGKHGFEDARKPYMEDGIEHLEMI
                     RPD"
     gene            complement(956..1897)
                     /locus_tag="PLB25_18665"
     CDS             complement(956..1897)
                     /locus_tag="PLB25_18665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019575503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HOE43628.1"
                     /translation="MTNLILHHYPMSPFSEKIRAVLGFKQLAWQSVIIPTIMPKPDLL
                     ALTGGYRKTPVLQIGADIYCDTALICDVLEQIAPTPTLYPAPVKGLALVLAQWADNEL
                     FWAAMGHNLKRPGIAQLFAGQAPEVAKAFGADRSAMAQGMARPRPADATVAYRSYLRR
                     LSGMLAGQDFLLGSAPCVADFAAYHPLWFTLTQTPVMADILQATSLVRAWLDRMAAFG
                     HGQSSPLTATEAIAVSATGTRAKGQFDAFFQDDHGIALGSRVSITPESFGTEPSVGEL
                     IAASRTRYTLRHEDPRAGVLHVHFPRVGYALKLEPAA"
     gene            complement(1930..2946)
                     /locus_tag="PLB25_18670"
     CDS             complement(1930..2946)
                     /locus_tag="PLB25_18670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HOE43629.1"
                     /translation="MPINQQILLDNRPQGEAVASNFRQVSSPTPELLDGQVLVRHHYL
                     SLDPYMRGRMNDSRSYTAAQPLGVVMGGGTAGEVIESRHAKFAIGDLVVGMGGWQQYS
                     VVNASEPGALRKVDTRHVPLSAYLGAVGMPGVTAWYGLGKIIEPKAGETMVVSAATGA
                     VGSAFAALSKARGCRTVGIAGGPDKCQYAVAELGFDACIDYKLHANAGSLSKALKEAC
                     PNGIDGHFENVGGMVLDAVLGRMNAFGRIAVCGMIAGYDGAPLPLSHPALILVSRLKV
                     QGFIVTEHMDVWPEALAELGALVASGKLRPRESIAQGIESAPEAFLGLLKGRNFGKQL
                     VKLI"
     gene            complement(3042..>3335)
                     /locus_tag="PLB25_18675"
     CDS             complement(3042..>3335)
                     /locus_tag="PLB25_18675"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008904343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOE43630.1"
                     /translation="ALMTLLDATLAAAARSVQKEVGMVTIEMKTSFMRPARGPLVAKG
                     HLLHRTATLGFTEATVFDAHGRACAHATGTYKFVRRLATGPKSINDLQPIPTD"
BASE COUNT          670 a         1054 c         1006 g          605 t
ORIGIN      
        1 aggcttccca ggtcaggcag gaggcgaaga ccccggcgtt gttgaacacc agatgcggtg
       61 cgccaaagcg ctcgaagaca acccgcccca gtgcctcaac ctcggcggct tgcgacacat
      121 cgagccttac cgccagtacc tcggcaccgg cagcgacgag ttcggcctgc gctgcatcca
      181 gcgcgtcttg ctgcacgtca accagcacca ggttcatgcc caggcgcgcg ccaagacgtg
      241 ccatctccag cccaaagccg gagccagcac cggtcagcac agcagttttg tgtttgaaat
      301 cagtgatcaa ggcggtctcc ggtcagacta tggtggaaaa aaattctcgt gacccttgtg
      361 gtgaaacgaa ctgctggcaa ttggcaacgg cacattgcgc gcaaacccga cgggccagat
      421 ggcgaagtga ttgatcaaca cgtcaatgaa tctgcggtgc tgcccattca gtccgggcga
      481 atcatttcca gatgctcaat gccgtcttcc atatagggct tgcgagcatc ttcaaagcca
      541 tgtttgccat aaaaggcggc caactgcagt tgcgcgccga tgcgaatcgg tcgcggaccc
      601 cagagctggc tgatggcggt cacggtctgc tcaatcagcg tgtgccccaa gccggtgcca
      661 cgcgcgctct ggcgcaccag cacccggcca aagctggcct cgggaaactt gataccggct
      721 tcaaaacagc gcgcataggc ctgcaattgg ccatgttgca cccccagcag atgcatggct
      781 tgcgggtcat agccgtccat gtcatgaaag acgcactgct gctcgaccac aaacacctcg
      841 ctgcgcaggc gcagcaccgc ataaagttcg gacacccgca ggtcatcgaa agccagcaat
      901 cgccatacca gcgcgttcga gcgagtgccc ccgccaatgc tccagagttt gaaggtcatg
      961 ctgcaggctc cagtttgagt gcatacccga cgcgcggaaa atgcacgtgc agcacaccgg
     1021 cacgcggatc ttcatggcgc aaggtatatc gggtgcggct ggccgcgatg agttcgccaa
     1081 cgctgggctc agtgccaaaa ctctctggcg tgatgctgac ccggctgccc agcgcaatcc
     1141 cgtggtcgtc ctgaaaaaaa gcatcaaatt ggcctttagc cctagtccct gtagcgctga
     1201 cagctattgc ctctgtagca gtcaaagggc tggactggcc gtgaccaaaa gccgccatgc
     1261 ggtccagcca ggcccgcacc aaagacgtag cctgcaaaat gtcggccatc accggcgtct
     1321 gggtgagcgt gaaccacagc gggtggtaag cggcaaagtc cgccacgcaa ggcgcactgc
     1381 cgagcaaaaa gtcctgcccg gcgagcatgc ccgacaggcg gcgcaggtaa gagcggtagg
     1441 ccacggtggc atcggccggt cgcggccgcg ccatgccttg cgccatggca gagcggtcgg
     1501 ccccaaacgc tttggcgact tctggcgctt gcccggcgaa caactgcgcg atgcccgggc
     1561 gcttcaggtt gtgccccatg gccgcccaga acagctcatt gtcagcccac tgggccagca
     1621 ccagcgccag ccccttgacg ggtgccgggt aaagcgtcgg tgtcggggcg atctgctcca
     1681 gcacgtcaca gatcagcgcg gtgtcgcagt agatgtcggc accgatttgc agcaccggtg
     1741 tcttgcggta gccaccggtc agcgccagca aatcgggctt gggcatgatg gtcgggatga
     1801 tgaccgactg ccaggcgagt tgtttgaaac ccagcaccgc gcggattttt tccgaaaacg
     1861 gtgacatcgg gtaatggtgc aggatgaggt ttgtcatggt ccaaggctcc ggtaagcgcc
     1921 gcgcgccggt cagatcagct tgaccagttg cttgccaaag ttgcgcccct tgagcaaacc
     1981 cagaaacgcc tcgggtgctg actcaatgcc ctgggcaatt gactcgcggg ggcgcaattt
     2041 gccgctggcc accagcgcgc ccagttcggc cagcgcctcg ggccagacat ccatgtgctc
     2101 ggtgacgata aacccttgca ccttgagacg gctgaccagg atcagcgccg ggtggcttaa
     2161 cggcagcggt gcaccgtcat aaccggcaat catgccgcac acggcgatgc ggccaaaagc
     2221 attcatgcga cccagcaccg cgtccagcac catgccaccg acgttctcga aatggccgtc
     2281 aatgccgttc gggcaggctt ctttaagtgc tttcgacagc gaaccggcat tggcgtgcag
     2341 cttgtagtca atgcaggcat caaagccgag ttcagccacg gcgtattggc atttgtccgg
     2401 cccgccagca atgcccacgg tgcggcagcc gcgcgccttg ctcaaggcgg caaaagcgct
     2461 gcccacggca ccggtggcag cgctcaccac catggtttcg ccggccttgg gttcaatgat
     2521 cttgcccaga ccgtaccagg ccgtcacccc cggcatgcca accgcaccca gataggcact
     2581 gagcggaaca tggcgggtat cgaccttgcg cagggcaccc ggttcactgg cattgaccac
     2641 actgtattgc tgccagccgc ccatgccgac caccaggtcg ccaatggcaa acttggcatg
     2701 cctggattcg atcacctcgc cggcggtacc gccgcccatc acgacaccaa gcggctgggc
     2761 cgcggtgtag ctcctggaat cgttcatgcg cccgcgcatg taggggtcca ggctgaggta
     2821 gtggtggcgc accagcacct ggccgtccag cagctccggg gtcgggctgc tgacttgcct
     2881 gaaattgctg gccactgcct cgccctgcgg gcggttgtcg agcaagattt gttggttaat
     2941 gggcatgagg gtactccagg tgaacacatg tgtgctattt aataaagagc tgtcagcgca
     3001 gtatccacgg gggctacagg ctgttttgat gcttttgttg gtcaatcggt cggaatcggc
     3061 tgcaaatcgt tgatgctttt cggcccggtg gccaggcgac gcacaaactt gtaggtgccg
     3121 gtggcatggg cacacgccct gccatgcgcg tcaaagaccg tggcctcggt aaaccccagt
     3181 gtggcggtgc ggtgcagcag atgccccttg gccaccagcg gaccacgcgc tggccgcatg
     3241 aaactggttt tcatctcgat ggtcaccatg ccgacctctt tttgcacgct gcgcgccgct
     3301 gcagccaggg tggcatcgag cagcgtcatc agggc
//
LOCUS       DAOSLL010000549         3334 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_24956_length_3334_cov_4.198841, whole genome shotgun sequence.
ACCESSION   DAOSLL010000549 DAOSLL010000000
VERSION     DAOSLL010000549.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24956_length_3334_cov_4.198841"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..525
                     /locus_tag="PLB25_18680"
     CDS             172..525
                     /locus_tag="PLB25_18680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005799242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43631.1"
                     /translation="MSKHLLIIFIALLLSACDLLGLGPDPRIAQKEADAKATGGACRH
                     GLRSIEDCYTLNKGVNKASIYAGWREMDEYMRENKLEGLRAEIALPAPVAAPAPGEEV
                     VEDTKAKPKAKAAAH"
     gene            complement(583..2022)
                     /gene="rlmD"
                     /locus_tag="PLB25_18685"
     CDS             complement(583..2022)
                     /gene="rlmD"
                     /locus_tag="PLB25_18685"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010458086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HOE43632.1"
                     /translation="MTEKTDASAKDAAPPDWLLVESLDLDAQGVAHKADGKVVFIEGA
                     LPFELVSANINRHKNNWEQGTLTAVHRSSSQRVEPHCPHFGLHTGACGGCKMQHLHIA
                     AQVAVKQRALEDNLWHLGKVKCGQILRPIEGPTWGYRFRARLSVRFVRKRGTVLVGFH
                     ERKSRYVADMQVCPVLPPHVDALLMPLRALVFGMEAAETCPQIELACGDTVTALVLRH
                     LEPLSVADLERLREFARQHHGVQWWLQAKGPETVCLLDPQVEPLRYALPEFGIVMPFK
                     PTDFTQVNPHINRVLVSRALGLLDVRRTERVIDWFCGLGNFTLPIATQAREVLGLEGS
                     EALVARSRENYVNNSAPAHARRALSATTFVARNLFEMTPEVLVADGIADKWLVDPPRE
                     GAFALVRSLADLHQQRLGTSDGTATPGAAGWQPPKRIVYVSCNPSTLARDAGLLVHQA
                     GYRCSAAGVVNMFPHTAHIESVAVFDLQA"
     gene            complement(2081..2929)
                     /locus_tag="PLB25_18690"
     CDS             complement(2081..2929)
                     /locus_tag="PLB25_18690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HOE43633.1"
                     /translation="MKRQFGLTALAVLSALTLAACSSTSLNRAPVEDRTTRPTAPLAA
                     GSTTDLAPVKLPQGYENAGKPGYYTVKPGDTLLRISLENGQSMHDVARWSHVENPNRI
                     EVGQVLRVVPPAAGEVVIKPIAKPTVTASAAGLAASAAAAPASVASAPVAAVKPAATD
                     SEINWVWPAGGVVLAGFDELKNKGLDIGGVAGEPVIAAADGRVVYVGAGLRGYGNLII
                     LKHDNVFLSAYAHNQTLLVKEDQAVQKGQKIAEMGSSDADRVKLHFEVRRQGKPVDPV
                     KYLPPR"
     gene            complement(2954..>3334)
                     /locus_tag="PLB25_18695"
     CDS             complement(2954..>3334)
                     /locus_tag="PLB25_18695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019655893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HOE43634.1"
                     /translation="GYQAAVLSHLCTEVYSMERLRGLHDKARDNLRHLRLANVHLLFG
                     DGMVGYPKGAPYAGIISAAGGEAVPQAWLDQLAVGARLVAPVAGGAAGSGAQSLVVIE
                     KTPLGLRQTVMEAVRFVPLKSGVA"
BASE COUNT          738 a         1020 c          907 g          669 t
ORIGIN      
        1 tgcgcccaaa tccgccggtg caaatagggg aagttatttc tgaccacatc cttaggccca
       61 atcggctgaa caggaaggcc cttaggggcc ttttttattg ctttgctgat attcattgat
      121 ggtcgaatca gctacataat ttcgttggca ttactcttta accttttcgt catgtccaaa
      181 catttgctga tcattttcat agccttgctg ctgtcggcct gtgatctgct cggtctgggc
      241 ccggacccga gaattgccca gaaagaggcc gatgccaaag ccactggtgg cgcatgccgc
      301 catggtttgc gcagcattga agactgctac accctcaata agggggtcaa caaggcatcg
      361 atctatgccg gctggcgtga aatggacgag tacatgcggg agaacaaact cgaaggtttg
      421 cgcgctgaaa ttgctctgcc agcaccggtt gctgcacctg caccgggtga ggaagtcgtt
      481 gaagatacca aagccaagcc gaaagccaag gccgctgcac actgaagcgg tcggcctgcg
      541 ccaggcagtg tgatgcggcc cctttgattg cggggccgga tgtcacgcct gaaggtcgaa
      601 aacagccaca ctctcaatgt gggcggtgtg cggaaacatg ttgacaacac ctgccgcaga
      661 gcaccgataa cctgcctgat gcaccagcag gcccgcgtct cttgccaggg ttgacgggtt
      721 gcaactgacg taaacaatgc gcttgggcgg ttgccagccc gctgcgccgg gcgtcgctgt
      781 accgtcagat gtaccaagac gctgttgatg caaatcggcc agtgatcgca ccagcgcaaa
      841 ggccccttcc cgaggcggat cgaccagcca tttgtcagca atgccatctg ccaccagcac
      901 ctcgggtgtc atttcaaata gatttcttgc tacaaatgta gtagctgaca gcgcacgtcg
      961 cgcatgggct ggagcactgt tattcacata attttcgcgg gaacgagcga ccagggcttc
     1021 actgccctcc agcccaagca cttcacgcgc ctgggtggca atcggcaggg tgaaattgcc
     1081 aagcccgcaa aaccagtcaa ttacccgctc agtgcgacgt acatcaagca ggccaagcgc
     1141 gcgtgacacc agcacccggt tgatatgcgg gttgacctgg gtgaaatcgg tcggcttgaa
     1201 cggcatgaca atgccaaatt ctggcagcgc gtagcgcaat ggctcaacct gcgggtccag
     1261 caagcatacg gtttctggcc ctttggcctg tagccaccat tgcacaccgt ggtgttgccg
     1321 ggcaaattcc cgcaagcgtt ccagatcggc cacactcagc ggctccaggt ggcgcagcac
     1381 cagcgcggtg accgtgtcac cgcaggccaa ttcaatttgc gggcaggttt cagccgcttc
     1441 catcccgaat accaaggcgc gcaagggcat cagcagggca tccacatggg gcggcagcac
     1501 cggacaaacc tgcatgtcag ccacataacg gctcttgcgc tcatgaaacc cgaccagcac
     1561 ggtgcccctt ttgcgcacaa agcgcactga caagcgcgca cggaaccgat agccccaggt
     1621 cggaccctcg atcggtctga gtatttgtcc gcacttgacc ttgcccaaat gccacagatt
     1681 gtcttccagc gcccgctgct tcacagcgac ctgcgcagcg atgtgcaaat gctgcatctt
     1741 gcaaccgccg caagccccgg tgtgcaagcc aaaatgcggg caatgcggct cgacgcgttg
     1801 cgacgacgaa cggtgaaccg cagtcagtgt gccctgctcc cagttatttt tgtggcggtt
     1861 gatattggcg ctgacaagct caaatggcaa agcaccctca atgaaaacca ccttgccgtc
     1921 ggctttatgg gccacgcctt gtgcatcaag atcaagcgat tcgaccaaga gccagtccgg
     1981 gggggcagca tctttggcag acgcatcagt tttttcagtc atgcaattct cagcaaacgc
     2041 cagttgcccg gcttgaaccg ggtcgcaagc caaaacagtt ctaccggggt ggcagatact
     2101 tgacaggatc aaccggcttg ccctggcgac gcacctcaaa gtgaagtttc acccgatctg
     2161 catcgctgct ccccatttcg gcaatctttt gccccttttg cactgcctga tcttctttga
     2221 ccagcaaggt ttgattgtgc gcataggcgc tcagaaatac attgtcgtgc ttgagaatga
     2281 tcaggttgcc gtagccccgc agcccggcac caacatagac gacccgtccg tcggcagccg
     2341 ctatcaccgg ctcaccggcg acaccgccaa tgtccagacc cttgttcttg agctcatcaa
     2401 aaccagccaa gaccacgccg ccggctggcc acacccaatt gatttcacta tcggtcgctg
     2461 ctggtttgac agcagccacc ggggcactgg cgacagaagc tggcgcggca gcggcgctcg
     2521 cggccaagcc tgcagcgctg gccgtcacag ttggttttgc aatgggtttg atcaccacct
     2581 caccggctgc cggtggcacc accctcaata cctgacccac ttcgatgcgg ttggggtttt
     2641 caacgtggct ccagcgtgcc acgtcatgca tcgactggcc attctccaga gaaatccgca
     2701 gcaaagtgtc acctggcttg accgtgtaat agccaggctt accggcgttt tcatagccct
     2761 gcggcaactt gaccggtgcc agatcggtgg ttgaacctgc ggcaagcggc gcagtcggtc
     2821 gcgtcgtgcg atcttcaacc ggtgcacgat tgaggctggt ggaactgcaa gcggccagcg
     2881 tcaatgcaga caacacggcc aacgctgtca agccaaactg gcgtttcata aaaatcatat
     2941 ttgtatatcc cgatcaagca accccggatt ttaaggggac gaaacgaact gcttccatca
     3001 ccgtctggcg cagacccagc ggggtttttt caatcacgac cagcgactgg gccccggaac
     3061 ctgcggcacc gccagcgacc ggtgccacca gacgcgcgcc aactgccaat tgatcgagcc
     3121 aggcctgagg caccgcttcc ccgcccgcgg cagaaatgat accggcatac ggagcgccct
     3181 ttgggtagcc aaccatgcca tcgccgaaaa gcaggtgaac attggcaagt cgtaaatgcc
     3241 gcaaattgtc gcgtgccttg tcgtgcaaac cacgcagccg ttccatcgaa tacacctcgg
     3301 tgcaaagatg gctcaaaacc gcggcctgat agcc
//
LOCUS       DAOSLL010000550         3322 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_25069_length_3322_cov_4.201102, whole genome shotgun sequence.
ACCESSION   DAOSLL010000550 DAOSLL010000000
VERSION     DAOSLL010000550.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25069_length_3322_cov_4.201102"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="PLB25_18700"
     CDS             complement(<1..611)
                     /locus_tag="PLB25_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014725601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOE43635.1"
                     /translation="MFTTELATVSYIGATILFILSLGGLSNQETALRGNLYGMVGMTI
                     AVLATVFGPQVTGAGIPWIIGAMVIGGSIGLYAARTVQMTQMPELVALMHSMVGLAAM
                     LVGFSTFIDPEASKGFVGAAKSIHEMEIYIGILIGAVTFSGSIVAFGKLSGRIGGNPL
                     LLPARHWLNLLGLLVVIWFGREFMNAHSISEGMTPLIIMTVIAL"
     gene            complement(624..2285)
                     /locus_tag="PLB25_18705"
     CDS             complement(624..2285)
                     /locus_tag="PLB25_18705"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HOE43636.1"
                     /translation="MQASATTDGSSGKVVVASTTEVVKPVQRIGVPREVFPGEKRVAT
                     VPEVVEKLIKLGFAVSVESGAGDAANFSDDTYRAAGAQIVDGAARLWAESDIVFKVCV
                     PTAAEIDLMREGGILIGFIWPAQNPELMQQLTAKKATVLAIDSLPRMLSRAQKMDALT
                     SQAGVAGYRAVVEAANAFGRFFAGQITAAGKVQPAKVFIAGAGVAGLAAIGTAVGLGA
                     IVRANDTRAEVADQVISMGGEFVKVDYEEEGGGGGGYAKVMSEGFQQAQRDMYAKQAR
                     EVDIIITTALIPGKPAPKLITAEMVKSMKPGSVIVDMAAERGGNCELTEPGLAVVKHG
                     VTIVGYTDLASRLAKQSSTLYATNLFRLAEELCKTKDGVVNVNMEDDAIRGLTVTKEG
                     SITWPPPAPNLPAAATAPAKPAAAPAAKKGGHGHGGASEPMAGSKLAIMFGVAAALFW
                     LVGASAPPAFLSHFTVFVLACFVGYMVVWNVKPALHTPLMSVTNAISSIIAIGALVQI
                     SPMGGAVDRPNELITWVAGAGIVLTAINMFGGFAVTQRMLAMFRK"
     gene            2405..2920
                     /locus_tag="PLB25_18710"
     CDS             2405..2920
                     /locus_tag="PLB25_18710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HOE43637.1"
                     /translation="MNTRWKPSTTVAAIIEQDGKFLLVEEHTSEGLRLNNPAGHLDEG
                     ESLTQACQRETLEETMHLFVPTVLLGVYMSRFARPAQATEAAQDVTYLRFAFYGRLGP
                     LQVGRTLDTGIVRTLWLSADEVRASADRHRSPLVLRCMEDYLAGQGFPLTLIHTDPSV
                     HGHQLVRRGIA"
     gene            2917..>3322
                     /gene="mnmA"
                     /locus_tag="PLB25_18715"
     CDS             2917..>3322
                     /gene="mnmA"
                     /locus_tag="PLB25_18715"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011797722.1"
                     /note="catalyzes a sulfuration reaction to synthesize
                     2-thiouridine at the U34 position of tRNAs; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOE43638.1"
                     /translation="MTASDPHRRVVVGLSGGVDSAVTAWLLKQQGHEVVGIFMKNWED
                     DDDNAYCSSNVDFVDAAAVADVIGIEIEHVNFAAEYKDRVFAGFLREYSSGRTPNPDI
                     MCNAEIKFKAFLDHAMRLGAEKIATGHYARTRF"
BASE COUNT          678 a         1001 c          992 g          651 t
ORIGIN      
        1 agggcgatca ccgtcatgat gatcagcggg gtcatgcctt cggaaatact gtgggcattc
       61 atgaactcgc ggccaaacca gatcaccacc agcagaccca gcagattgag ccaatgccgt
      121 gccggcagca gcagcggatt gccgccgatg cggcccgaga gcttgccaaa ggccacgatg
      181 gagccggaga aggtcaccgc gccaatcagg atgccgatgt agatctccat ttcatgaatt
      241 gacttggcgg caccgacaaa tcccttggaa gcctccgggt cgatgaaggt ggaaaagccc
      301 accaacatcg cggccaggcc caccatgctg tgcatcagtg ccaccaattc gggcatctgt
      361 gtcatttgca cggtgcgtgc tgcgtacaag ccgatactgc cgccgatcac catggcacca
      421 atgatccagg ggatgccggc accggtgacc tgggggccaa aaacggtcgc cagcacagca
      481 atggtcatgc cgaccatgcc atagagattg ccgcgcaagg cggtttcctg gttcgacaaa
      541 ccgcccaggc tcaagatgaa aagaatcgtc gctccaatgt aggagacggt tgccagttct
      601 gtggtaaaca tgaattgcct cccttatttg cgaaacatcg ccagcatgcg ctgggtgacg
      661 gcaaagccgc cgaacatatt gatggcagtc agcacaatgc cggcacctgc aacccaggtg
      721 atcagctcat tgggccggtc cacggcaccg cccattggtg aaatctgcac cagggcacca
      781 atcgcgatga tgctgctgat ggcgttggtc acgctcatca gcggggtatg caaggcaggc
      841 ttgacgttcc acaccaccat gtagccgaca aagcaggcca gcacaaacac cgtgaagtgc
      901 gacaaaaatg ccggcggtgc actcgcaccc accagccaga acaaggctgc tgccacacca
      961 aacatgatcg caagcttgct gccggccatc ggctcgctgg cgccgccgtg cccgtggcca
     1021 cccttcttgg ccgcaggtgc tgcggcaggc tttgcaggcg cagttgcagc agccggcagg
     1081 ttgggtgccg gtggtggcca ggtgatgctg ccttccttgg tgacggtcag gccgcggatg
     1141 gcatcgtcct ccatgttgac gttcaccacg ccatccttgg tcttgcacaa ctcttctgcc
     1201 aggcgaaaca ggttggtggc gtacagcgtt gatgactgct tggcaagacg cgaggccagg
     1261 tcggtatagc cgacgatggt cacgccgtgc ttgaccacgg ccaggccggg ctcggtcaac
     1321 tcgcagttgc cgccccgttc agccgccatg tcaacgatca cgctgccggg tttcatgctc
     1381 ttgaccatct cggcagtgat gagcttgggt gcgggcttgc cgggaatcag cgcggtggtg
     1441 atgatgatgt ccacctccct ggcctgcttg gcgtacatgt cgcgctgggc ctgctggaag
     1501 ccctcgctca tcaccttggc atagccgccg ccgccgccgc cttcttcctc ataatcaacc
     1561 ttgacgaatt cgccgcccat cgagatgacc tggtcggcca cttcggcacg ggtgtcgttg
     1621 gcacgcacga tggcacccag gccaacggca gtgcctatcg ctgccaggcc agccacaccg
     1681 gcaccggcaa tgaagacctt ggcgggctgg accttgcccg ccgccgtgat ctgtccggca
     1741 aagaagcggc caaaggcgtt ggcggcttcg acgacagcac ggtagcctgc cacaccagcc
     1801 tgggaggtca gtgcgtccat tttctgggcc cggctgagca tgcggggcag cgagtcgatg
     1861 gccagcacgg tcgctttctt ggccgtgagc tgctgcatca attccgggtt ttgcgcgggc
     1921 cagatgaagc caatcaggat gccgccttct cgcatcaggt cgatctcggc agcggttggc
     1981 acacacacct tgaacacgat gtctgactcg gcccaaagcc tggcagcgcc atcgacaatc
     2041 tgggcaccgg cggcgcgata ggtgtcgtca gagaagttgg cggcatcccc cgcacccgac
     2101 tcgaccgaca ccgcgaaccc gagcttgatc agtttttcga ccacctctgg cacggtggca
     2161 acacgttttt cgccagggaa tacctctcgg ggtactccga tgcgctgcac cggtttcaca
     2221 acctctgtcg tagaggcaac aacaacttta cctgatgagc catcagtagt ggcactcgct
     2281 tgcatggggg cgactcctcg gatttatttg aagtgcgcga gcttacatca gttttttgca
     2341 ttttctgagg caccttgtat gcagtcaggt attcctggtc tgacagactt ttggataatc
     2401 aggcatgaac acacgatgga aacccagcac cacagtggcg gcgatcattg aacaagacgg
     2461 caaattcctg ttggtggaag aacacacctc tgaaggtttg aggctcaaca acccggccgg
     2521 tcacctcgat gagggggaaa gcctgacgca ggcgtgccag cgcgaaacac tggaggaaac
     2581 catgcatttg tttgtgccca ccgttctgct gggcgtgtac atgtcgcgat tcgcgcgtcc
     2641 ggcgcaggca actgaggccg cgcaagatgt cacttatctg cgttttgcgt tttacggcag
     2701 gctcgggccg ttgcaggtgg gcagaacgct tgacaccggc attgttcgca ccttgtggtt
     2761 gagcgccgac gaagtccggg ccagcgccga tcggcatcgc agtccgctgg tgttgcgctg
     2821 catggaagat tacctggccg ggcagggttt tccgctgacc ctgattcata ccgacccatc
     2881 ggtgcatggc catcaactgg tccgccgagg catcgcatga ctgccagcga ccctcatcgc
     2941 cgtgtcgtgg tcggactcag cggcggggtg gactcggccg tcacggcctg gctgctcaag
     3001 cagcaggggc atgaggtggt gggcatcttc atgaagaatt gggaagacga tgatgacaac
     3061 gcctattgct catcgaatgt tgactttgtc gatgccgcgg ccgtggcgga tgtgattggc
     3121 atcgagatcg agcacgtcaa ctttgccgca gagtacaaag accgggtgtt tgcaggtttt
     3181 ttacgtgagt acagcagtgg ccgcacaccc aaccccgaca tcatgtgcaa cgcagagatc
     3241 aagttcaagg cctttctgga ccacgcgatg cggctagggg ccgaaaaaat tgccaccggc
     3301 cactacgcgc gaacccgctt ca
//
LOCUS       DAOSLL010000551         3306 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_25230_length_3306_cov_3.779452, whole genome shotgun sequence.
ACCESSION   DAOSLL010000551 DAOSLL010000000
VERSION     DAOSLL010000551.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25230_length_3306_cov_3.779452"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1186
                     /locus_tag="PLB25_18720"
     CDS             <1..1186
                     /locus_tag="PLB25_18720"
                     /inference="COORDINATES: protein motif:HMM:NF012580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE43639.1"
                     /translation="AGNTPTVEQLDALAEWLNARPDFDSSERPAYVTDSLARDYPDGR
                     AIANVASGVIAVRVSRLRRDLILWFRPQTQQTISWAGNPADKPQVVGPNGMRLTPRRS
                     FELFIESVQERSLPWSALEVESALRLRQLVMELVISQTVKLTEQNIDLTASNEELDAF
                     AYVASHDLKEPLRGIHRYAHQLLESTQTPTPEDRQRLDSLMRLTLRMDSLLNSLLHFS
                     RVGRTNLEFEAVDLNELVEDALEMIGVRKHEATCRISIPRPLPTIECNPVRVREIFSN
                     LVSNAIKYNLNDQPWVELIYFAASDESAHQNVPRQAQGQIIYGVRDDGIGIEQRHFDQ
                     IFRMFKRLHGRDEFGGGMGAGLTVVQKVVQRHGGAIWLESTPGQGSCFYFTLPGTYKD
                     SL"
     gene            1183..1680
                     /locus_tag="PLB25_18725"
     CDS             1183..1680
                     /locus_tag="PLB25_18725"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE43640.1"
                     /translation="MSGADTFQSTAHVAAVSRLRRAHPVLVVEDSDEDFDTVLTAARL
                     AGIQHEIRRVLTGDDCLRLLRGTAGHPITQVALVLLDLNTPHGDGRSALEHIHADPQL
                     NAIPLVVLSTSANPRDVDFCYGHGANAYHVKPVSHSEHLLVLQTLLNYWLGSVVLPTA
                     QTLER"
     gene            1680..>3306
                     /locus_tag="PLB25_18730"
     CDS             1680..>3306
                     /locus_tag="PLB25_18730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE43641.1"
                     /translation="MSPPTHWHILIIDDNPDDRAEFRRMLIAGSGRTCRFAEAELGAT
                     GLQMFLDNQAQSLSGTGPLFDCVLLDFHLPDIDGTQWLSALCGASGMPPCPVVVMTGW
                     DDIDGSDGPNVLQAGAQDYIGKSWTNEQSLRRAIENSIERYKLRQTHDRAAKALAHTE
                     ERYFTLFKSIDEGFCLIELIYDEQENPIDWRFVEVNPAFEKQNGLPGATGKRIRELAP
                     DIEPFWFKTYGEVARTGKPIRFQDEVKALGELWFDVYAFRIGAPGSYLVAVLFKDITE
                     HKNAEKATQAASQYARSLIEASLDPLVTISAQGKITDVNNATEKVSGANRDELIGSDF
                     ANYFTDPQKAREGYQQVFKQGSVTDYPLAMRHVTGKITDVLYNASLYRDAQGNVMGVF
                     AAARDITERKKVEKDLRESEYRWRFAIDGPGDGLWDWDIQNNQAFYSERWKAMLGLES
                     SDIGSNPLVWASHLHPEDKDRVLAEVQSHLDWQTPNYNSEHRVICNDGSAKWMLGRGL
                     VVQRDGSGKPLRMIGTNTNITERKRVEAALLASN"
BASE COUNT          747 a          945 c          968 g          646 t
ORIGIN      
        1 cgcaggcaat acacccacgg tggagcagct cgacgcgctg gcggagtggc tcaacgcccg
       61 gcccgacttt gactccagcg aacgcccggc ctacgtcacc gacagcctgg cccgcgacta
      121 tcccgacgga cgggcgattg ccaatgttgc aagcggcgtg attgcggtgc gcgtgtcgcg
      181 gctgcggcgt gaccttatcc tgtggttcag gccccaaacg cagcaaacca tctcttgggc
      241 tggcaacccg gcagacaagc cgcaagtcgt cggccccaac ggcatgcggc tgacaccgcg
      301 gcgctcgttc gagttgttca ttgagtcggt gcaagagcgc tccttgccct ggagtgcgct
      361 ggaagttgaa tctgcgttgc gcctgcgcca attggtgatg gagttggtga tttcgcaaac
      421 ggtaaagctg acagaacaaa acatcgacct aactgccagt aacgaagagc tagatgcatt
      481 tgcctacgtt gcttcccatg atctcaaaga gccgctgcgt ggcatccacc gatacgctca
      541 ccagttattg gagagcacac aaacccccac gccagaagac cgccagcgcc ttgacagcct
      601 gatgcgcctg accctgcgca tggacagcct gctcaactcg ctgctgcact tctctcgggt
      661 tggccggacc aatttggagt ttgaggcggt cgacctgaat gagttggtgg aagacgcgct
      721 ggagatgatt ggcgtgcgca aacacgaggc cacctgccgc atcagcatcc ctaggccttt
      781 gcccaccatt gaatgcaacc cggtgcgggt gcgtgagatt ttcagcaatc tggtcagcaa
      841 cgccatcaag tacaacctga acgaccaacc atgggtggag ctgatctact tcgcagcctc
      901 cgatgaatca gcgcaccaga acgtaccccg acaggcccag gggcagatca tttacggcgt
      961 acgcgacgat ggcattggca ttgagcaacg gcattttgac caaatctttc ggatgttcaa
     1021 gcgcctgcat gggcgcgacg aatttggcgg cggcatgggc gcgggcctga ccgtggtgca
     1081 aaaagtggtg cagcgccacg gcggcgctat ctggctggaa tcgacaccgg gccagggcag
     1141 ttgcttttac ttcacactgc ccggaacgta caaggactcc ctgtgagcgg cgccgacaca
     1201 tttcaatcca cagcccatgt tgcagccgtg agtcgcctgc gcagggcgca tccggttttg
     1261 gtggtggaag actcggacga agactttgac accgtgctca ccgctgcccg gctggccggt
     1321 atccaacacg agatacgccg ggttctgacg ggcgacgact gcctgcgact gctgcgaggc
     1381 actgccggcc accccataac gcaagtcgcg ctggtgctgc tagacctcaa taccccacac
     1441 ggggatggtc gcagcgcact ggagcacatt cacgcagacc cccagctcaa cgccattcca
     1501 ctggtggtct tgagcacctc cgccaacccg cgtgatgtgg atttttgcta tggccacggg
     1561 gccaatgcct accacgtcaa gccggtgagc cacagtgaac atctgttggt gctgcaaacc
     1621 ttgttgaact actggctagg cagcgtggtg ttgcctaccg ctcaaacatt ggagcggtaa
     1681 tgagccctcc cacacactgg cacatcctga tcattgacga caaccccgat gaccgggccg
     1741 aattcaggcg catgttgatt gcggggtctg ggcgcacctg ccgcttcgcc gaggccgaac
     1801 tcggtgcaac cggtttgcaa atgtttctgg acaatcaggc gcagagcctc tcaggcacag
     1861 ggccgctgtt tgactgcgtg ttgctagact ttcatttgcc tgacatcgac ggcacgcaat
     1921 ggctgagcgc cctttgcggc gcgtcgggca tgccaccgtg tcccgtagtg gttatgacgg
     1981 gctgggacga tatagatggc agtgacgggc caaacgtatt gcaagccggg gcgcaagact
     2041 acatcggtaa aagctggacc aacgagcaga gcttgcgccg cgccattgaa aattcgattg
     2101 agcgctataa gctgcgccaa acccacgacc gtgccgccaa agcgctggcg cacactgaag
     2161 agcgctactt cacactattc aaatcaattg atgaaggctt ttgccttatc gagttgattt
     2221 atgacgagca ggaaaacccg atcgactggc gcttcgtgga agtgaatcca gctttcgaaa
     2281 agcaaaatgg gctacccgga gccactggga aaaggattcg cgaactggca ccggatattg
     2341 aaccgttttg gttcaagacc tacggtgaag ttgcccggac gggcaagccg atccggttcc
     2401 aggacgaagt gaaagcgttg ggtgagctct ggttcgatgt ctacgccttc cggatcggcg
     2461 cgcccggcag ctacttggtc gccgtcctgt tcaaggacat caccgaacac aagaatgcgg
     2521 aaaaagcgac ccaagctgca tcgcaatacg cccgcagctt gatcgaagcg agtcttgatc
     2581 cgctagtgac gatcagcgcg caaggcaaga tcactgacgt caacaatgcc acggaaaaag
     2641 tatctggcgc aaaccgggac gagctgatcg gcagtgactt tgccaactac ttcaccgatc
     2701 cgcagaaagc ccgtgagggg tatcaacagg tgttcaagca aggctcagtg actgactacc
     2761 cgctggcgat gcgtcatgtc actggcaaga tcacggatgt cctttacaac gccagcctgt
     2821 accgtgatgc tcagggcaat gtcatgggtg tcttcgctgc ggcgcgtgac atcacggaac
     2881 gaaagaaagt cgaaaaagac ttgcgtgaaa gtgagtaccg ctggcgattt gccattgacg
     2941 gtccgggtga cggtttgtgg gactgggaca ttcaaaacaa ccaggctttc tacagcgaac
     3001 gctggaaagc catgctgggt ttggagagca gtgacattgg cagcaatcct ttggtgtggg
     3061 cctctcactt gcatccggaa gacaaagatc gtgttttggc ggaagtgcag tctcatttgg
     3121 actggcagac acccaactac aacagcgagc atcgcgtcat ctgcaacgat ggcagtgcga
     3181 aatggatgct ggggcgtggt ctcgttgtcc aaagagatgg aagtgggaag ccgcttcgca
     3241 tgatcggaac caacaccaac atcacagagc gtaagcgggt tgaagctgca ctgcttgcca
     3301 gcaata
//
LOCUS       DAOSLL010000552         3300 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_25275_length_3300_cov_5.173498, whole genome shotgun sequence.
ACCESSION   DAOSLL010000552 DAOSLL010000000
VERSION     DAOSLL010000552.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25275_length_3300_cov_5.173498"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="PLB25_18735"
     CDS             complement(<1..1063)
                     /locus_tag="PLB25_18735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOE43642.1"
                     /translation="MNNLPGLNFQLGEEIDALRDAVREFAQVEIAPRAFEIDRSDQFP
                     MDLWEKMGSLGVLGITVGEEYGGANMGYLAHMIAMEEISRASASVGLSYGAHSNLCVN
                     QIKRNGTPAQRAKYLPKLISGQHVGALAMSETGAGSDVISMRLKAQDQGGYYLLNGSK
                     MWITNGPDADTLVVYAKTEPELGARGVTAFLIEKGMKGFSIAQKLDKLGMRGSHTGEL
                     VFQNVEVPESHILGGLNMGAKVLMSGLDYERAVLTGGPLGIMQSVMDNVVPYIHDRKQ
                     FGQSIGEFQLVQGKVADMYTVLQAGRSFAYTVAKNLDMLGQEHVRQVRKDCASVILWC
                     AEKATWMAGEGVQIFGGNGY"
     gene            complement(1103..1555)
                     /locus_tag="PLB25_18740"
     CDS             complement(1103..1555)
                     /locus_tag="PLB25_18740"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011799876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HOE43643.1"
                     /translation="MTLTPTHHHHETPLQRVQASFERQGLMQHLGARVLHATPGECVI
                     CLPYSDKVTQQQGAFHGGAIGALADIAAGYAALTQAAHGMEVVSVEYKINFLTSRQGG
                     ELRATGRVRKAGQRIIVTSAEVMHVADDGLETLCAVLQQTVMPVAKHY"
     gene            complement(1578..2057)
                     /locus_tag="PLB25_18745"
     CDS             complement(1578..2057)
                     /locus_tag="PLB25_18745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HOE43644.1"
                     /translation="MPPTYSISDLAKEFDLTTRAIRFYEDMGLLQPERSGPGGRNRIY
                     SVRDRARLKLTLRAKRLGLSLTEAKEIIDMYDSPRDTVAQLDRFLLVLSLHQQRLDAQ
                     MLDLQANMDELKVHQREAKAMLAKAQRRAAGNTSAAGKRNQSLDDTVQGQGTSAVHA"
     gene            2224..3249
                     /locus_tag="PLB25_18750"
     CDS             2224..3249
                     /locus_tag="PLB25_18750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HOE43645.1"
                     /translation="MKKTMLAASCLTFAVASVQAQSNVQLSGLVDVYAGSMRMAGDAS
                     SKSVVGSGGLTTSWWGMTGTEDLGGGMKASFALTAFFRADTGEIGRFVNDPQFSRDAN
                     VSLSGGFGTVTLGRGKAPNFLPTIAFNPMGDSFTFSPLVLHADVPLFNGTKWAATTPS
                     DTGWANQITYSTPSFSGLKANLHYQLGEVASDTGKNNVGFNLMYAAGPLGLTAFWERD
                     QITNPVPALFAGSATRTDWMLGGSWDATVVKAFLTYGESKSDQSNVKAKTTTLGASVP
                     LGNGKLLADWANTRLSGGGATRDTVAIGYDYTMSKRTDLYAVLMNDKITNFTSGNSFG
                     VGVRHRF"
BASE COUNT          637 a         1008 c          957 g          698 t
ORIGIN      
        1 tgtagccatt gccgccaaaa atctgcaccc cttcgccggc catccaggtg gctttttcag
       61 cgcaccacaa aatcaccgaa gcacagtcct tgcgcacctg gcgcacatgc tcttgcccca
      121 gcatgtcgag gtttttggcc accgtgtagg caaatgaccg acccgcctgc agcacggtgt
      181 acatgtcggc gaccttgccc tgaaccaact ggaattcgcc aatgctctgg ccaaactgct
      241 tgcggtcgtg aatgtacggc accacgttgt ccatcacgct ttgcatgatg cctagcggcc
      301 cgccggtgag cacggcgcgc tcgtagtcca acccgctcat cagcaccttg gcgcccatgt
      361 tcaggccacc gaggatgtgc gactccggca cttcgacatt ttgaaacacc aactcaccag
      421 tatggctgcc gcgcatgccc agcttgtcga gcttttgcgc aatggaaaat cccttcatgc
      481 ccttttcgat caaaaaggcg gtgaccccgc gcgcaccgag ttcaggctcg gtcttggcat
      541 agaccaccag ggtatcggca tccgggccgt tggtgatcca catcttgctg ccattcaaga
      601 ggtaatagcc gccctgatcc tgtgccttga ggcgcatcga aatgacatcc gacccggccc
      661 cggtttcgct catggccagc gcgcccacgt gctggccact gatcagtttg ggcaggtact
      721 tggcacgctg ggccggcgtg ccattgcgct tgatctggtt cacgcacaag ttgctgtgcg
      781 caccgtagct caggccgacc gacgcactgg cacgcgatat ttcctccatc gcaatcatgt
      841 gcgccagata acccatgttg gcgccgccgt attcctcccc gacggtgatg cccagcacgc
      901 ccaacgaccc catcttttcc cacaggtcca tcggaaactg gtcagaccgg tcaatctcaa
      961 aggcacgtgg ggcgatctcg acctgggcaa attcgcgcac cgcatcgcgc agcgcgtcaa
     1021 tctcttcacc gagttgaaag ttgagtccgg gtaaattgtt cacgttgtgt ctcctggttt
     1081 tgattgaatg cgaattctca gatcagtagt gctttgccac tggcatcacg gtttgctgca
     1141 gcacggcgca aagggtttca aggccgtcgt cggccacatg catgacttcg gcgctggtaa
     1201 caatgatgcg ctgaccggct ttgcgcacgc ggccggtggc gcgcagctcg ccaccttgac
     1261 ggctggtcag aaaattgatc ttgtactcga cgctgaccac ttccatgccg tgcgccgcct
     1321 gggtcaaggc ggcatagcca gccgcgatat cggccagcgc gccaatcgcg ccaccatgaa
     1381 aagcaccctg ctgctgcgtc accttgtcgg aatagggcaa gcaaatcaca cactcgccgg
     1441 gcgtggcgtg cagaacgcgg gcacccaaat gctgcatcag cccctggcgc tcaaagctgg
     1501 cttgcacccg ttgcagcggg gtttcgtggt ggtggtgggt gggggtcagg gtcatgcggc
     1561 accgtttttg gaaaagatca ggcgtgcaca gcggacgtac cctggccctg gacggtgtca
     1621 tccagggact ggttacgttt gcccgccgcg ctggtgttgc cagcggcgcg gcgttgcgct
     1681 tttgccagca tggctttggc ttcgcgctgg tgaactttca gctcatccat gttggcctgc
     1741 aaatcgagca tctgcgcgtc aaggcgctgc tggtgcaggc taagcaccag caaaaaccgg
     1801 tcaagctggg ccaccgtgtc gcgcgggctg tcgtacatgt cgatgatctc tttggcctcg
     1861 gtcagactca ggcccaggcg tttggcacgc agcgtcagct tcagccgggc ccggtcgcgc
     1921 acgctgtaga tgcggttgcg cccaccgggg ccgctgcgct cgggttgcag cagccccatg
     1981 tcttcgtaga agcggatggc ccgggtggtc aggtcaaact ctttggccag gtcgctgatg
     2041 ctgtaagtgg gaggcatggg cttgggatgc gttcagttga cgttgacgtt aacgtcaatg
     2101 ggagcgtgga ttatggcgca caaaaacgac aaaagaaccg gcacagactt tgctagtatc
     2161 ggcttggacg cagccctttg gggcacacca acccaccgtt tcaccaactt aacaggattt
     2221 cccatgaaaa aaaccatgct cgcagcaagc tgtctgacat ttgccgtcgc aagcgtgcag
     2281 gcgcaaagca atgttcaact gtcgggtctg gtcgatgtct atgctggctc catgcgcatg
     2341 gcaggcgacg cgtccagcaa atcagtggtc ggcagcggcg gcctgaccac ctcatggtgg
     2401 ggcatgactg gcaccgagga tttgggtggg ggcatgaagg cgagctttgc cttgaccgcg
     2461 ttttttcggg cagataccgg cgagatcggc cgttttgtca acgacccgca gttctcgcgc
     2521 gatgccaatg tgagcctgtc gggtggcttt ggcacggtca ccctggggcg tggcaaggca
     2581 cccaacttcc tgcccaccat tgcattcaat ccaatgggtg actcgtttac cttttcgcca
     2641 ctggtgctgc atgccgatgt gcctttgttc aacggcacaa aatgggctgc caccacaccc
     2701 tcggacaccg gctgggccaa ccagatcact tactcaaccc ccagtttcag tgggctgaag
     2761 gccaatttgc actaccagct cggtgaagtc gcctcagata ccggcaagaa caatgttggc
     2821 tttaacctga tgtacgccgc tggccccctg ggcctgaccg ctttctggga gcgcgaccag
     2881 atcaccaatc cggtgccagc gctgtttgca ggcagtgcca cccgaaccga ctggatgctt
     2941 ggcggctcct gggacgcgac cgtggtcaag gcctttttga cctatggcga gtccaaatcc
     3001 gaccaatcca atgtcaaggc caagaccacc acgctgggtg cgtcggtacc gcttggcaac
     3061 ggcaagttgc tggctgactg ggccaacacc agactcagtg gcggtggtgc cacacgcgac
     3121 acggtggcga ttggttacga ctacaccatg tccaagcgca ccgacctgta tgccgtcttg
     3181 atgaacgaca agatcaccaa cttcacctca ggcaacagct ttggcgtggg tgttcggcac
     3241 cggttttaag gatgtggtcg ggaataactc cccgttttgc accaaggagt tgggatgcac
//
LOCUS       DAOSLL010000553         3299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_25284_length_3299_cov_5.039766, whole genome shotgun sequence.
ACCESSION   DAOSLL010000553 DAOSLL010000000
VERSION     DAOSLL010000553.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25284_length_3299_cov_5.039766"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="PLB25_18755"
     CDS             <1..1059
                     /locus_tag="PLB25_18755"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017875382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HOE43646.1"
                     /translation="AVLIDLEDTSASVPLLSRLLAAAALPMPVGDLTPQVSASVGVTF
                     YPQAQDIDADQLLRQADQAMYQAKVAGKNRYCVFDAAQDNSMRVHHESLERIRLALAQ
                     HEFVLHYQPKVNLHSGQVMGAEALIRWQHPEKGLLAPSAFLPVIEDHPLAVAVGEWVL
                     DSALTQMEQWHDAALDLPVSVNISARQLQQGNFVQQLQAILARHPQVNPAHLALEVLE
                     TSALADMAQVSQVIEDCARMGVMFALDDFGTGYSSLTYLKRLRVALLKIDQSFVRDML
                     DDPDDLAILQGIIGLAAAFKRDVIAEGVETVAHGTALLKLGCALAQGYGIARPMPPEK
                     LPAWVAAWQPDAAWRALR"
     gene            1183..2229
                     /locus_tag="PLB25_18760"
     CDS             1183..2229
                     /locus_tag="PLB25_18760"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOE43647.1"
                     /translation="MKKVISFLAVGLALVAAMTSVQGQVETLPSGPPAQSAKTVVFVA
                     SDYRNGGVMGVYRGFEEASQKLGWQIRLEDGRGQKATQGAILAQALAARPHGIVFGGF
                     DPDDFADPVADAKQNKVILVGWHAAKDPGPTKDLFVNVSTKPVDVAKNAADFVIRDAI
                     ARRRQVGVVIFNDNQYAVANAKTDAMKATIQACRGYSACRVLSVENVLISEAAAAMPG
                     VVPKLAAAHGAAWTYSLAINDVYFDEINYPLVLARRTDIRNVSAGDGSTKALGRIAAG
                     ISQQVATIAEPLKMQGYQLADELNRAFAGVLPSGFQSRPILVTTELLSAAGNRGIEST
                     LGFEAAYAAIWGRK"
     gene            2232..>3299
                     /locus_tag="PLB25_18765"
     CDS             2232..>3299
                     /locus_tag="PLB25_18765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE43648.1"
                     /translation="MKFLRYSVLALRVLAWALLASFAVFAVATAFGIAYERDKALHDA
                     HADAQEVVEQSLPAITASLWQYDVAGLNALLGGMVASTVVVRVELLSLDKLVVDVSQP
                     GFTGPIDRVWTVAVMAQDQKASIGTLRISESYASTNAQVTETLKTLVMTDLVKIIGLA
                     LVLFAIVYRKIARHLHQLAMDVTRLGRSGDAGKLTIRRKQRINYRDELDILVDAINNF
                     VTERKSADQALRIAATAFESQQGVIITNAQRVILRVNKAFTSITGYSAEEAVGQSPRI
                     FKSGRQDSDFYAAMYQALDTTGAWAGEIWNRRKNGEIYPEWLNISAVNDATGQTTHFV
                     AIFSDISERINAQAQIDTLAFY"
BASE COUNT          665 a          919 c         1026 g          689 t
ORIGIN      
        1 gcggtgctga tcgacctgga agacacgtcg gccagcgtgc ccctgctgag tcggctgctg
       61 gcggcggctg cactgccgat gccggtgggc gacctgacgc cacaggtttc ggccagcgtg
      121 ggcgtcacct tttacccgca ggcccaagac attgatgccg accagctgtt gcgccaggca
      181 gatcaggcca tgtaccaggc caaggtggcg ggcaagaacc gttactgtgt gtttgatgcc
      241 gcgcaggaca acagcatgcg cgtccaccac gaaagcctgg agcgcatccg cctggcgctg
      301 gcgcagcatg agtttgtgct gcactaccag cccaaggtca acttgcacag cggccaggtg
      361 atgggtgccg aggcgctgat ccgctggcag catcccgaaa aaggtctgct ggcaccgtcg
      421 gccttcctgc cggtgattga agaccacccg ctggccgttg ccgtgggcga gtgggtgttg
      481 gacagcgccc tgacccaaat ggagcagtgg cacgatgccg ctctggatct gccggtcagc
      541 gtcaacatca gtgcgcgcca gttgcagcaa ggcaactttg tgcagcagtt gcaagccatc
      601 ctggccaggc acccgcaagt caacccggcc catctggcgc ttgaggtgct ggagaccagc
      661 gcgctggcgg acatggcgca ggtgtcgcag gtcattgaag actgcgcccg gatgggggtg
      721 atgtttgcgc tggacgactt tggcaccggc tactcgtcgc tgacctacct caaacgcctg
      781 cgcgtggctt tgctgaaaat cgaccagagc tttgtgcgcg acatgctcga tgaccccgat
      841 gacctggcca ttttgcaagg catcattggc ctggctgccg ccttcaagcg cgacgtgatt
      901 gccgaaggcg tggagacggt ggcgcatggc actgcgctgc tgaaactggg ctgtgcgttg
      961 gcgcaaggct atggcattgc ccgtcccatg ccgcctgaaa agttacccgc ctgggtcgcc
     1021 gcctggcagc ccgatgcggc ctggcgtgca ctgcgctagc gacgatggcg caggccactt
     1081 tgcatcaggc tttcaacaaa tccgtgacat acatcaacat cacaagcagg tgctgatgga
     1141 caagctttgg tcggcgagct tttgagttta caaggagaaa acatgaagaa ggtaatcagt
     1201 tttcttgcag tcgggttggc gctggttgct gccatgacat cggtacaggg tcaagttgaa
     1261 acgctgccat ccggaccgcc tgcgcagtct gcgaagaccg tcgtttttgt ggcctctgac
     1321 taccggaacg gtggtgtcat gggggtttat cggggctttg aggaggcctc gcaaaagctg
     1381 ggctggcaga tacgtctcga agacgggcga gggcaaaagg caacccaggg ggcgattctg
     1441 gcgcaagcac ttgctgcccg tccgcacggg atcgtgtttg gtggttttga cccggacgac
     1501 tttgctgatc cggtggcgga tgccaaacaa aacaaggtca ttttggtggg gtggcacgca
     1561 gccaaagacc cgggtccgac caaagacctg ttcgtcaacg tctcgaccaa accggttgat
     1621 gttgcaaaaa acgctgccga cttcgtgatt cgggatgcca ttgccaggcg acggcaggtt
     1681 ggggtcgtca tttttaacga caaccagtac gcggtggcca atgccaaaac cgatgccatg
     1741 aaagccacca ttcaggcctg ccggggctac agcgcttgca gggtgttgtc ggtagaaaat
     1801 gtgctgatct ctgaggccgc tgcagcaatg cctggtgtcg ttcccaagct ggctgcggcc
     1861 catggggcgg cgtggactta cagtctggcg atcaacgatg tctatttcga tgaaatcaac
     1921 tatccgctgg tgctggcccg gcgcaccgat attcgcaatg tgtccgccgg ggatggctcc
     1981 accaaggcgc ttggccgcat tgctgccggc atttcccaac aggtggccac gattgccgag
     2041 cctctgaaaa tgcaggggta ccagcttgcc gatgaactca atcgtgcctt tgcgggtgtc
     2101 ttgccgagtg gtttccagtc aagacccatt cttgtcacca cggagcttct tagcgccgca
     2161 ggcaaccggg gcatcgaatc caccttgggt ttcgaagctg cgtatgctgc gatctggggc
     2221 aggaagtagc gatgaaattt ctgcgctact ccgtccttgc gctcagagtc ctggcttggg
     2281 cgttgttggc aagttttgct gtttttgccg tggcgacggc attcggtatt gcgtatgaac
     2341 gcgacaaggc cctgcacgac gcgcatgctg atgcccagga ggtcgttgaa caaagcctcc
     2401 cggcgatcac tgcgtcgctg tggcagtacg acgttgcagg gcttaatgcg ttgctgggcg
     2461 gcatggtggc atcgacggtt gtggtacgcg tcgagttgct gagcctggat aaactggtgg
     2521 ttgatgtcag ccaaccgggt tttaccggtc cgatagaccg ggtgtggact gttgcggtca
     2581 tggcgcagga tcaaaaagcg agcataggaa ctttgcgcat ttcagagtct tacgccagca
     2641 ccaacgcgca ggtcactgag acgctgaaaa cactggtgat gaccgacctg gtcaagatca
     2701 tcggtttggc cctggtgctg tttgccattg tctatcgcaa gattgccagg cacctgcacc
     2761 aactggccat ggatgtgacc cgacttgggc gttcgggtga tgcaggcaaa ctgaccatcc
     2821 gccgaaagca gcgtatcaat taccgcgatg aactcgacat tctggtggat gctatcaaca
     2881 actttgtgac agaacgcaag agtgctgacc aagcactgcg cattgccgcc actgcgtttg
     2941 agtcacagca aggcgtgatc atcaccaacg cgcagcgcgt gatattgcgg gtgaacaagg
     3001 cctttaccag cattaccggt tacagcgctg aagaagcggt ggggcagagc ccgcgcattt
     3061 ttaaatccgg tcgtcaggac agcgatttct atgcggccat gtaccaggca cttgacacga
     3121 caggtgcctg ggccggtgaa atctggaacc ggcgcaagaa tggcgagatc taccccgaat
     3181 ggctcaatat cagcgcggtc aacgacgcta ccggccagac cacccatttt gttgccatct
     3241 tcagcgacat cagcgagcgc atcaacgcgc aggcgcaaat tgacacgctg gctttttat
//
LOCUS       DAOSLL010000554         3299 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_25290_length_3299_cov_1.946363, whole genome shotgun sequence.
ACCESSION   DAOSLL010000554 DAOSLL010000000
VERSION     DAOSLL010000554.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25290_length_3299_cov_1.946363"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="PLB25_18770"
     CDS             complement(<1..534)
                     /locus_tag="PLB25_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43649.1"
                     /translation="MKLKHPLRFLDQIKTSEQLRAYLDSLLYVGEMAAHFPGPGPYGP
                     GVSEIAGFDTLEKAGIYRFAESWKTELRRWINETQDPATGFWGARIGNATKWRQKADI
                     NATFHVLKFVLTESGENQSSQYPLRYAGKLAKGVLGTMSSAIPEGESKQHDWGLTQYQ
                     GAKMLTKYLWPHLSESEK"
     assembly_gap    644..653
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<654..972)
                     /locus_tag="PLB25_18775"
     CDS             complement(<654..972)
                     /locus_tag="PLB25_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43650.1"
                     /translation="MRERIVRIAMALTGLVLLVAGFAIYSTISSVPQLFRRGAELKAQ
                     GYYVGEFEFKMVASQYYLNEGRYLDAYQTLRRITNEMATLRGLNKMPLKATPEEQMAF
                     LLEQ"
     gene            complement(1175..2428)
                     /gene="icd"
                     /locus_tag="PLB25_18780"
     CDS             complement(1175..2428)
                     /gene="icd"
                     /locus_tag="PLB25_18780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOE43651.1"
                     /translation="MYQHIKVPSEGQKITVNADMSLNVPDQPIIPFIEGDGTGLDITP
                     VMIKVVDAAVAKAYGGKKKIHWMEVYAGEKSTNVYGPDVWLPEETLKALREYVVSIKG
                     PLTTPIGGGIRSLNVALRQELDLYVCLRPIQYFEGVPSPVKEPHKTNMVIFRENSEDI
                     YAGIEFEAESPKAQKIIKFLQEEMGVTKIRFPNTSGIGVKPVSREGTERLVRKAIQYA
                     IDNDKPSVTLVHKGNIMKFTEGGFRDWGYGLAQKEFGAQLIDGGPWCSFQNPKTGKTI
                     TIKDSIADAFLQQILLRPAEYSVIATLNLNGDYISDALAAQVGGIGIAPGANLSDSVA
                     CFEATHGTAPKYAGKDYVNPGSEILSAEMMLRHMGWLEAADAIIKSMERSIKSKRVTY
                     DFARLMEGATQVSCSGFGQVMIEHM"
     gene            complement(2587..2820)
                     /locus_tag="PLB25_18785"
     CDS             complement(2587..2820)
                     /locus_tag="PLB25_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43652.1"
                     /translation="MEALTLAGLAAFSALAGAAASAFAGAAASTLAGAAAAAGAEAAA
                     KEPAAKRPATRAARSLFIFSFLEDNGNTKDLQI"
     gene            2973..>3299
                     /locus_tag="PLB25_18790"
     CDS             2973..>3299
                     /locus_tag="PLB25_18790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HOE43653.1"
                     /translation="MLALAATCCSVSHAQSGPQLDLPRVQITAGMYLIDTQVAATPQQ
                     RSTGLMFRQQMPQGEGMLFVFDRPSEQCFWMKNTLLPLTAAFVADDGTIVNLEDMKPQ
                     TTESHCS"
BASE COUNT          697 a          944 c          848 g          800 t
ORIGIN      
        1 tttctcgctc tcagatagat gtggccaaag atacttggtc aacatcttgg cgccctgata
       61 ctgcgtcaag ccccagtcat gttgctttga ttcgccttcg ggaatggcgc tagacatcgt
      121 acccagaacc cccttggcca gttttcctgc atagcgaagc ggatattgcg agctctggtt
      181 ttcacccgac tctgtaagca caaacttcag gacatgaaaa gtcgcattga tatccgcctt
      241 ctgccgccac ttcgtagcgt tcccgatacg tgctccccag aagccagtgg cagggtcctg
      301 cgtctcattg atccagcggc ggagttcggt tttccacgac tcggcgaatc gatagatgcc
      361 agctttctcc agagtgtcaa atcccgcaat ctctgaaaca ccaggcccgt aaggtccggg
      421 gccaggaaaa tgagcagcca tttcgccgac gtataacaag gagtcgaggt aggcccggag
      481 ttgttccgat gtcttgatct gatcgagaaa gcggagtgga tgtttgagct tcaagggtcg
      541 ccccacctcc tgacttagct ttgtcagcgc ttcaacgacg ttacatgtcg gcgcgaaata
      601 tgaaaacgct gggtagcggt gatccatgaa agcgcccgtg actnnnnnnn nnngctgctc
      661 aagcagaaaa gccatttgct cttctggcgt tgctttgagc ggcatcttgt tcaaaccccg
      721 caaggttgcc atctcgttgg tgatgcgtcg caaggtttgg taagcgtcaa gatagcgacc
      781 ttcattcaga tagtactggc tggcgaccat cttgaattcg aactcgccca cgtagtaacc
      841 ctgtgctttc agttcggcac cccgcctgaa cagctgtggc acgctgctta tggtcgagta
      901 aatggcaaat ccggcaacca gcagcacgag gccggtgagt gccatcgcaa ttcggacgat
      961 acgttctctc atatccaacc ctgctgtatg tcaaatgcgg gttcaatctg aaacgatcaa
     1021 agattcttgt gcttcccgtt gtgggactgg gcgtctgaga agctgaccgc gacgcttgtt
     1081 ctcccactgt ccccagttgg aaaccagaca aacgcccgcg agaatcaact cgctggcgtt
     1141 gtcgtttcgc cgtcaatgac gactttgcag cctttcacat gtgctcaatc atcacctgtc
     1201 caaagcccga gcagctgacc tgggtggcac cttccatcag gcgggcgaaa tcgtaggtga
     1261 cgcgtttgct cttgatggag cgttccatcg acttgatgat ggcatctgca gcttcgagcc
     1321 atcccatgtg gcgcagcatc atctcggccg acaaaatttc cgaacccggg ttcacgtagt
     1381 ctttgccagc atacttgggt gccgtgccgt gggtggcctc aaagcaggcc actgaatcag
     1441 acaaattggc acccggcgca ataccaatgc cgcccacctg agcggccagg gcatcagaga
     1501 tgtaatcacc attcagattc agcgtggcaa taacgctgta ctcggccgga cgcaacaaaa
     1561 tttgctgcag gaacgcgtcg gcaatgctgt ccttgatggt gatggtcttg ccggtttttg
     1621 ggttctggaa actgcaccat ggcccgccat cgatcagctg cgcgccaaac tctttctgcg
     1681 ccaggccata accccaatcc cggaatcctc cttcggtgaa cttcatgatg ttgcccttgt
     1741 gaacaagggt cacgctgggc ttgtcgttgt cgatggcata ctggatggcc ttgcgcacca
     1801 ggcgttctgt gccttcacgt gacactggtt tgacaccgat gccggaagtg tttgggaagc
     1861 gaatcttggt cacacccatt tcttcctgca agaacttgat gattttttgc gccttgggtg
     1921 actcggcttc gaactcgatg ccggcataaa tgtcttccga attctcacgg aagatcacca
     1981 tgtttgtctt gtgcggctcc ttcaccgggc tgggcacacc ttcaaaatac tggattgggc
     2041 gcagacagac atacaggtca agctcctggc gcagcgccac gttgagcgac cgaatgcccc
     2101 caccaatggg cgtggtcaag ggccctttga tagacaccac atattcgcgc agtgccttca
     2161 gcgtttcttc tggcagccac acatccgggc cgtagacgtt ggtggatttt tcacccgcgt
     2221 aaacctccat ccaatgaatt ttcttcttgc caccgtaggc cttggccacc gcggcgtcca
     2281 ccaccttgat catcacggga gtgatgtcca gcccggtgcc gtcaccttca ataaagggaa
     2341 tgatgggttg gtcaggcaca ttcagcgaca tgtcggcatt gacggtgatt ttttggcctt
     2401 cagaaggcac cttgatgtgt tgatacatgg tgtgaggtct ccgtgagtgg atcaaatacc
     2461 ctgcttatgt aaggtgcctg cctgggcaac acccgggtgg gcgccaaact ttttcattct
     2521 agtccgatat attttccgaa tgttgtcaac cgacctgtca gcacgttcgt tgtagcttca
     2581 ccttcttcaa atttgaaggt cttttgtatt accgttatct tcaaggaaac tgaaaatgaa
     2641 caaacttctc gccgctctgg ttgctggtct cttcgctgct ggttctttcg ctgctgcttc
     2701 ggcacctgcc gctgctgctg cacctgccaa agttgaagct gctgcacctg cgaaggctga
     2761 agccgctgct cctgccaagg ctgaaaaggc tgccaagccc gccaaggtca aagcttccaa
     2821 ggccaagaaa gccgctccca aggcttcgat gtaatccagc ctcagcgctt gatttcaaga
     2881 aatgcccgca tcagcgggca ttttttcgtc cattttttgt ttacagtggc cagatgcaac
     2941 aacacctctc catcacctac ttcatttccg gaatgctggc gctggcggcc acctgctgca
     3001 gtgtcagcca cgcccagagc gggccgcaac tggatttgcc ccgcgttcag atcaccgcag
     3061 gcatgtatct gatcgataca caagtggctg ccacgccgca gcagcgctcc accggcctca
     3121 tgttccgcca gcaaatgcct cagggtgaag gcatgctgtt tgtattcgac cgaccgagcg
     3181 agcagtgctt ctggatgaaa aacaccttgc tgcccctgac ggcggctttt gtggcggacg
     3241 acggcaccat cgtcaacctg gaagacatga agcctcagac caccgaatca cattgctcg
//
LOCUS       DAOSLL010000555         3295 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_25327_length_3295_cov_4.219753, whole genome shotgun sequence.
ACCESSION   DAOSLL010000555 DAOSLL010000000
VERSION     DAOSLL010000555.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25327_length_3295_cov_4.219753"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="PLB25_18795"
     CDS             complement(<1..771)
                     /locus_tag="PLB25_18795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HOE43654.1"
                     /translation="MDAADALHLLDDEQEWSAFSRPDASRQGLWESNVLIEGMHCAAC
                     ALTVEDALKSVRGVVSAEVSAASQRGRIVWAASAVVPSKWMRAVRQSGYRAVPANDAF
                     ARERRKQESRKALWRMMVAGLCMMQVMMYAYPAYVAKPGDLTMEMEQLLRWASWVLTL
                     PVVIFSCAPFFVSAWRDVMHRQVSMDLPVALGMLITFAVSTAGAFDPAGIFGREVYFD
                     SLTMFVFFLLAGRWFELRLRERTAGALEALMNRLPDSIA"
     gene            956..1399
                     /locus_tag="PLB25_18800"
     CDS             956..1399
                     /locus_tag="PLB25_18800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HOE43655.1"
                     /translation="MYEKILVATDGSTLSKKAVSSAIALAALTGASLFAIKIVPRYPQ
                     SYFEGGLALQAAEVGRIEKQWADEGQAVVDEVKKEAELQGVKTRALTVKSDVVSEAII
                     SAAKKNKCDLIVMASHGRKGVKRLLLGSETHQVLTHSHIPVLVLR"
     gene            complement(1460..2503)
                     /locus_tag="PLB25_18805"
     CDS             complement(1460..2503)
                     /locus_tag="PLB25_18805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008326569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE43656.1"
                     /translation="MTYQVLIVDDAEINLVLFQALVKRMGNSQSITFSDALQGLAWAK
                     EQDFDLLIVDYMMPGLDGIEFIQQLRATADKQNVPILMITANDQKQIRYRALDAGATD
                     FLTKPVDKVEFMARANNMLQLGASRKALADRAAWLAEEVKKATAEIVRRERETVIRLS
                     KAAEYRDPETGAHILRMAHYSELIARALNLPKAEQELLLEAAPMHDIGKVGIADNILL
                     KPGRLTPEEFEVMKHHAAFGFEILKGSLSRVLQAGAEIARDHHEKYDGSGYPNGLKGT
                     AIPIFSRIVAVADVFDALTSERPYKKAWTLERASEHIKASSGMHFDPLCVEAFFSNWD
                     GVLEIRDHYKDGS"
     gene            complement(2588..>3295)
                     /locus_tag="PLB25_18810"
     CDS             complement(2588..>3295)
                     /locus_tag="PLB25_18810"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE43657.1"
                     /translation="AGDGQVALDLLAQRRFDLVLMDMMMPVMDGLEATRRIRAQERGE
                     ATPIVAMTANAMQGDRERCLQAGMNDYISKPIEIAELHRVVALFGRARAPAQPDDVDG
                     FASPRQSALGTRSADSGGGEFDYGVALRSSDQEVVDIISEVFVAQWPLDVEKMRQALA
                     HGDPVPLRHTAHALKGSLGMFGARPAVILAAELEQLAVGAPGLDAQDMLPIARDKLAE
                     LCGQVDLLLAALQSTPA"
BASE COUNT          754 a         1016 c          860 g          665 t
ORIGIN      
        1 cgcaatactg tcgggcagcc gattcatcag tgcttccagc gcaccggccg ttcgctcgcg
       61 caatcgcaac tcaaaccagc gccccgccag cagaaaaaaa acaaacatcg tcagcgaatc
      121 aaaatagacc tcacgcccaa agatgcctgc cgggtcgaaa gcacctgcag tgctgaccgc
      181 aaaggtaatc agcataccaa gggccaccgg taaatccatg ctgacctgac gatgcatcac
      241 atcgcgccag gcgctgacga aaaacggcgc acaggaaaaa atcaccacgg gcagcgtcag
      301 aacccaggaa gcccagcgca gcaattgctc catctccatc gtcaaatcac ctggctttgc
      361 cacataagcc gggtaggcgt acatcatgac ttgcatcatg caaagcccgg cgaccatcat
      421 gcgccacagc gccttgcgcg attcctgttt gcggcgttcc cgcgcgaagg catcgttggc
      481 gggaaccgcc cggtaaccag actgccgcac cgcccgcatc catttggagg gtacaaccgc
      541 cgacgcggcc cagacgatgc gcccgcgttg gcttgcggcg ctgacttccg cagaaacaac
      601 accacgcacg ctcttgagtg catcctccac cgtcagcgca caggccgcac agtgcatccc
      661 ctcaatcaga acattggatt cccacaaacc ttggcgcgat gcgtccggac ggctgaatgc
      721 cgaccattcc tgctcatcgt cgagcaggtg caatgcgtcg gcagcatcca tctgatccgc
      781 tccagacggt actgtcgcaa ggggttcgac cggggtgtca aatggttgat ggggaacgtt
      841 aggggacatg gccgcgaggg tatccgacat tgactgcata ttgcctgagc taaatcaagg
      901 tctgccgaaa gcgtaggggt atgctgtggt catcgaacct ccaggagatg ccaccatgta
      961 tgaaaaaatt ctcgttgcaa cagatggttc caccctgtcc aaaaaggctg tcagcagcgc
     1021 catcgcccta gccgcgctga ctggtgccag cctgttcgcc atcaagattg tgccgcgtta
     1081 cccgcaaagc tattttgaag gtgggttggc cctgcaagcc gccgaggtcg gtcgaatcga
     1141 aaagcaatgg gctgacgaag gacaggcagt cgtggatgaa gtcaaaaaag aagctgaact
     1201 tcagggtgtc aaaacccgcg ccttgacggt caagtccgat gtcgtctcag aggccattat
     1261 ttcagccgcc aaaaaaaaca aatgcgacct gattgtgatg gcatcgcacg ggcgtaaagg
     1321 tgtcaagcga ctgttactgg gcagtgaaac ccaccaggtc ctcacccact cgcacattcc
     1381 cgttctggtt ttaaggtaaa tcaggccgta gcccttattg agaaagcgcc agcagctaca
     1441 taataaatag caatctggtc tagctcccat ctttgtaatg gtcgcgaatt tcaagcacgc
     1501 cgtcccagtt cgagaaaaat gcctcgacac aaagcggatc aaagtgcatg ccagaacttg
     1561 ccttgatgtg ctcggacgca cgctcaagcg tccaggcttt cttgtaaggt cgctctgaag
     1621 tcagggcgtc aaagacatct gccaccgcca cgatccggct gaatatcggt attgccgtgc
     1681 ccttcaaccc attgggatag cctgagccat cgtatttttc atgatgatcg cgggcaattt
     1741 ctgcgccggc ttgcaacacc ctggacaagc tgcctttcaa aatctcaaag ccaaatgcgg
     1801 cgtggtgttt catcacttca aactcttcag gtgtcaacct tcccggtttg agcaaaatgt
     1861 tatcggcgat gccaaccttt ccgatgtcat gcatgggggc ggcttcgaga agcagttcct
     1921 gctcggcttt gggcaaattc agggcccgag caatcaactc tgaatagtgc gccatgcgca
     1981 gaatgtgagc accagtttcg gggtcgcggt actctgccgc cttggacagg cgaatgaccg
     2041 tctcgcgctc acgtcgcaca atctccgccg ttgccttctt gacctcttcg gccagccacg
     2101 ccgcccggtc tgccagcgcc ttgcggctag ctcccaattg cagcatgttg ttggcccgcg
     2161 ccatgaactc aaccttgtca actggcttgg tcagaaaatc ggtagcacca gcgtccagtg
     2221 cccggtagcg aatctgcttc tggtcattgg ccgtgatcat caaaatgggc acattctgct
     2281 tgtcagcggt ggcccgaagc tgctgaataa actcgatgcc gtcaagcccg ggcatcatgt
     2341 agtcaacaat caacaggtca aaatcctgtt ctttggccca agccaaaccc tgcaaggcat
     2401 ctgagaatgt gatggattgg ctgttgccca tgcgcttgac cagcgcttga aacaacacca
     2461 ggttgatctc ggcatcgtca acaatcagta cttggtaggt catggccaca ggtcttttca
     2521 acttggtttg agcgtttgcg cctgaggagg cctgggccaa gtgtcgcaaa ccgaaccact
     2581 ttaccactca ggcaggtgtg ctttgcaatg ccgccagcaa taaatcgacc tgcccgcaaa
     2641 gctcagccaa cttgtccctg gcgatcggca acatatcttg cgcgtccaaa cccggcgcgc
     2701 caacggccag ttgttcaagt tcagccgcca atatcacggc cggcctggca ccaaacatgc
     2761 ccaggctacc ttttagggcg tgcgcagtgt gacgcaacgg caccggatcg ccatgcgcga
     2821 gtgcctgccg catcttctcg acatccagtg gccactgcgc gacaaaaacc tcggaaatga
     2881 tgtccaccac ctcctggtca cttgatcgca gtgcaacgcc atagtcaaac tcaccgcccc
     2941 cggagtcagc cgaccgggtg ccaagcgcag actgccgagg gcttgcaaac ccgtccacat
     3001 cgtccggttg ggctggtgcc cgcgctcgcc caaacagcgc cacgacgcgg tgcaattcgg
     3061 caatttcaat gggttttgag atgtagtcgt tcattcccgc ctgcaaacag cgctcgcggt
     3121 ctccctgcat agcgttggcc gtcatggcca cgatcggcgt cgcttccccc cgctcttggg
     3181 cacggattcg gcgggtcgcc tccaggccgt ccatgacggg catcatcatg tccatcaaca
     3241 ccagatcgaa ccgccgctgc gccagcaagt ccaaggcaac ttgtccgtcg ccagc
//
LOCUS       DAOSLL010000556         3286 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_25417_length_3286_cov_4.769421, whole genome shotgun sequence.
ACCESSION   DAOSLL010000556 DAOSLL010000000
VERSION     DAOSLL010000556.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25417_length_3286_cov_4.769421"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(135..272)
                     /locus_tag="PLB25_18815"
     CDS             complement(135..272)
                     /locus_tag="PLB25_18815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43658.1"
                     /translation="MPTHKAIGVLIEEDGGPLIWPRITETEQNAFDETCRKLFKYINS
                     D"
     gene            complement(315..509)
                     /locus_tag="PLB25_18820"
     CDS             complement(315..509)
                     /locus_tag="PLB25_18820"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE43659.1"
                     /translation="MTRRKDEGTEITIEHHCSASIGVVLFVNHETPHEDILRWADAGM
                     YQAKDAGRNAIRSYEANGEN"
     gene            1356..1994
                     /locus_tag="PLB25_18825"
     CDS             1356..1994
                     /locus_tag="PLB25_18825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOE43660.1"
                     /translation="MSDALNYLIKTRPETISHYFAFLKQAGTHLDPKTRILISVITKV
                     HAQTDRGFRQYLGRALREGCTPMEVLDALLMAFPALGLAKIIWAVDIILDMKIPGFDP
                     DVITRTSPPPAQWRDVMASKDVPDGEVTRTEFDGRGLFVYREKKAFQVYDSHCPHQST
                     DITQLAINGTTLTCPKHQWEFDAKSGDCIKNGNTGLTRIECKVQRGRLLAFW"
     gene            2499..2687
                     /locus_tag="PLB25_18830"
     CDS             2499..2687
                     /locus_tag="PLB25_18830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43661.1"
                     /translation="MPIRLVAEVLGTIRKHARISTSSHQMRNQAHGTDIAQSPRLPEL
                     AANLIGMGNSSPTPFCFR"
BASE COUNT          707 a          902 c          899 g          778 t
ORIGIN      
        1 tggagctgga gttgccacag cctgatttgt caatttatga ccatgaccag ggtcacaaca
       61 caggatctga tgatgagtaa aaaagcggct cgttagcgga atttgtgggt agtttgacgc
      121 tgtcaagccg gtattcagtc tgaatttatg tacttgaaga gcttgcggca agtttcatcg
      181 aaggcgtttt gctctgtctc ggtaatgcgt ggccagatca gcggaccacc atcttcctca
      241 atcaatacac cgattgcctt gtgagtgggc attttcttga attttgggtc gccgtaatgt
      301 gggagtcaat cccttcaatt ttcaccattg gcttcataag accggatcgc attgcgcccc
      361 gcatccttgg cctggtacat gccggcatcg gcccatcgca ggatatcctc atgcggggtt
      421 tcatggttga caaacagcac cacgccaatg ctggccgaac aatggtgctc gatggtgatt
      481 tcagttccct cgtctttcct gcgggtcatg acgtaaggtg cagacaggga cgcgagaatt
      541 ttgtccgcta tcacctgggc ctgtgccctt gattcagcgc tatccacatc aagttcaccc
      601 aaccgagctg gacggcattg gcccactggc atcccatgcg aggtgtccat tttgtgtggc
      661 gagcagtctg gtcgcacggc ggctggcggg gctcggatcg ggctgctgat ttgttacctc
      721 ggggctcctg agcgcgactt gagttgggct gccatggtcg gcaccactgt tgcgcgaggt
      781 catcgcgctg ccggttttca tggtcggtct ggctgtttgt gccattgccc attcggcggg
      841 gacaggccac acacgctgga ttggtgcgcg aagcgcccgt ggcctgcctg atgccgcttg
      901 tctgcaccgg cttgattggc actgacatgc gccttattgg gcattctgcg tagaggaccc
      961 tgatcggggt gaattgttag ccggcctgtt gcagggcaac cgacataaca agtttgagaa
     1021 tgatctgaaa atgcggccat caacatggcg ctacgctgcc agttgcccct ggctaccgtg
     1081 ttgccgctgg tcagcaaacc ggaacttgaa gccatggcaa gcatgcgatg cgcgtcagca
     1141 tgcagcggtt gtgaaaacag cagagttccg ttgtgccgcc cacctgcccg accagcctgc
     1201 tggtcattaa catcaacatc ggccgtcgca gtcgcagcga tagccaaggc cacagcgggc
     1261 gtgcgatgat tggcggcgcg gcccggaaaa tcattggttt gtctgacaaa ccggtgctgg
     1321 tgcccgattt gacctgacaa cacgaagacc cgtccatgag tgatgcactg aactatctga
     1381 tcaagacccg gcctgaaacc atcagtcatt actttgcctt tttgaaacag gccggcaccc
     1441 acctcgatcc caagacgcgc attctgatct cggtgatcac caaggtgcac gcgcaaaccg
     1501 accgtggttt tcggcagtat ctgggccgcg ccctgcgcga gggctgcaca ccgatggaag
     1561 tgcttgatgc gctgctgatg gcttttccgg ccttgggcct ggccaagatc atctgggcgg
     1621 tggacatcat tctggacatg aagattcccg gctttgatcc tgatgtcatc acccgaacca
     1681 gcccgccgcc agcccagtgg cgtgatgtca tggccagcaa agatgttccc gatggtgaag
     1741 ttacccgtac cgagttcgac ggacgtgggc tgtttgtcta tcgcgaaaag aaagccttcc
     1801 aggtgtatga cagccactgc ccgcaccaaa gcaccgacat cacccaactg gcgatcaatg
     1861 gcaccaccct gacctgtcca aaacaccagt gggaatttga tgccaagagc ggtgactgca
     1921 tcaagaatgg caacaccggc ctcacacgca ttgaatgcaa agtgcaaagg ggtcgcttgc
     1981 tggcattctg gtagttgtct gcaatgcgcc ttggcggcac accaaaacca taacttgtag
     2041 cagattcgcg cagtcgattg cgccagatgc cagatctgaa attggctcag ttttttagct
     2101 aaatatgcct ctatcgctct ctggtattgc gtatcaagct atctaaccaa tagcatcttg
     2161 gaagctgatg ggcagaacgc ttgcggtgat tctgtcggtg actggaacgc gggctttcac
     2221 catgattcct cagttgaccg cttgttttca tctgtaagtc cttatgcgca tttacttctt
     2281 tggcttcgat ggggttcaac gaatgggtga caacgccagg cacccgattg agctactggc
     2341 aacgcgactg ggggcgtttc gccttcctcg tccgccagga cgacatgctc tgaagttgct
     2401 gagcgtcgtt gtcacgccca gccgcgccgc cagtggccca ctcgggcgct tccaactgaa
     2461 tccaggataa aaggacaatc actgagcaag gaaactccat gccgatccgg ttggttgccg
     2521 aggttcttgg aactatccgc aaacatgccc ggatttcaac gtcttcgcat caaatgcgca
     2581 accaggcgca cggcaccgat atcgcgcaat cgcctcgatt gccggaattg gcagccaacc
     2641 tgataggcat gggtaattcg agtccgactc ctttttgttt ccgttgatct gtgactggta
     2701 tgaatggcag agtcgatgta gtcgggtcgt ggccttcggg acattgcaat caaccgggcc
     2761 tgacctgcgt cgcactgact cacgggttcc cgatcagcct gagcccgtcg gtgcattcga
     2821 atagccttcg acaaaaccct tttcctttct tgggccgctt tgactacctg cccttgacgg
     2881 ttctgcgcaa aaagccttga aaaatgtgcc atctgcttgc cgccgcgaag cactggccgg
     2941 ataaatttca gcagtagcaa ttgatattgc agcggcttgg tgttgccaaa cgggcgctag
     3001 gcgcatttga gcttttggtg ataggcatca aactgcctgc tccaccaagc cgaattggcc
     3061 aacatgacat cttgaaacaa gctgtcgcgc gcaaggcaag gcaagataac acatggacag
     3121 ggcaaatttg ttctttcgcc aagtcgttga tttactgtca ttcccacgca ggcgggaatc
     3181 tagtgcagtc cggattcccg cctgcgtgcg aatgacgagg atgccataag aacaaattta
     3241 ccctgacaac ctggatgatt tggaaatgca ataaaaacaa gcgatc
//
LOCUS       DAOSLL010000557         3273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_25545_length_3273_cov_3.563083, whole genome shotgun sequence.
ACCESSION   DAOSLL010000557 DAOSLL010000000
VERSION     DAOSLL010000557.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25545_length_3273_cov_3.563083"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="PLB25_18835"
     CDS             complement(<1..182)
                     /locus_tag="PLB25_18835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43662.1"
                     /translation="MAVLVARATEVVVAGTDHAQCLQSTLSCPVVGLSFGAAHGGGTG
                     CVARLEFVFIPWLGCNR"
     gene            288..1409
                     /locus_tag="PLB25_18840"
     CDS             288..1409
                     /locus_tag="PLB25_18840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019657233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HOE43663.1"
                     /translation="MTTLGDLPRQTVARGDQLRRIFLKSGLLAVTLLPLIAHPSDIKN
                     APLRLRIVGGLAGANQFPRHEEPFWQKTLPALSRGQITTEIVPFDKAGIRTHEMLRLM
                     QLGVVPFGTMLMGGILSADPELAALDLAGMNPNMAALRRSVAAFRPHLEQTMRERHGI
                     EVLAIYAYPAQVTFCSRSFTGLADLAGRRVRISNPSQADLIRPFGAVPIQSEFSAVVS
                     GMRSGNVDCAITGAMSGNAIGLQEVASHLHTSATTWGLSVFGANLGAWTALPPDMKSL
                     IKTELPKLEAAIWADSERQTDEGVACNTGRGSCLTGKTGLMKEVQTNAVDESKLRISF
                     RDSVLPAWVQRCGNTCVPVWNRLLAPVTGIRAETQATRP"
     gene            1406..>3273
                     /locus_tag="PLB25_18845"
     CDS             1406..>3273
                     /locus_tag="PLB25_18845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HOE43664.1"
                     /translation="MICTHKKAETRGIAGSKRLALPDGLRCAGQSICTLRAIPMQGSL
                     RRVRWAVLSAATLLILIVMALATTLLYRDHAEALERARTLVTRVAQNSEADINRNLVS
                     VDMMLAELSIWITQERTETPPPSRGVQTLGVEKNLSRLVRAALQQNLMLRDIAVIASD
                     GSALIGARPSTQRLGLPMPAGFLDAVLQQPYAGLMVSAPTLNPLNSERVLYLARSQRL
                     PDGMVVAVVAELQLSLLSALLNPEDDSQRLTVTLEKESGQLLVSYPMVDVLIGQTLHP
                     ALESLKGGAGAQDMPGRLQAEPSVTAVHPTLYAGLLLSVGMPLRSALAQWQSQRNIVL
                     GGTAVLLFLILGGSWATWRGFSRMAAARAEVALANTRLQDANQQLAGSLSLVEATLEA
                     TGEAVLVIGNDGRIQHYNALLAVALRMPEAVLTSGDMAQLRNLLLDQLQNPAEYLDGN
                     ARAYASPTAEVRDQLVFKDGRVFQRHSLPQLLDGQVMGRVWSFQDVTAFKEAERKLQL
                     AASVFSHAHEGISITDAAGNIVDVNDTFIRITGYEREEVVGKNPRILQSGRQSPEYYA
                     AMWRELLEQGRWSGEVWNRRKSGEVYAEQLSISAVRDVDGKVQHYVSLFTDISSIK"
BASE COUNT          670 a          938 c          981 g          684 t
ORIGIN      
        1 cggttgcacc caagccaggg gatgaagaca aattcaagtc gcgcaacgca cccagtcccg
       61 ccgccgtgcg ccgcgccgaa cgagaggccc accaccgggc aactcagggt gctttgcaaa
      121 cactgcgcgt ggtcggtgcc agcgaccaca acctcagttg cccgagccac caagacggcc
      181 atacggtcga cgccagagtt ccaactgaat ttggttgtgg cccaaccgcg ttaagatgca
      241 aaaaagcttc tctgattctc aattttgcag aaacaagacg gttcaggatg acaacccttg
      301 gcgatttacc ccgacagact gtcgctcgcg gcgatcagct ccggcggatt ttcctgaagt
      361 ccggattgct ggcagtcacg ctgcttccat tgatcgcgca tccctctgac atcaagaacg
      421 caccgctgcg cctgcgcatc gtcggtggct tggccggagc caatcaattt ccgcgccacg
      481 aagagccgtt ttggcaaaag acgctgcctg cattgagtcg cggacaaatc acgacggaga
      541 ttgtcccgtt tgacaaagcc gggatccgca cccacgaaat gctgcgcctg atgcaactgg
      601 gtgtggtgcc ttttggaacc atgttgatgg gcggcatctt gtcggccgac cctgagctgg
      661 cggcactgga tcttgcgggc atgaacccca acatggcagc cctcagacgc tcggtcgcgg
      721 cttttcggcc tcacctggag caaacgatgc gtgagcgcca cggcatcgaa gttctggcga
      781 tttatgcgta tccagcgcag gtcactttct gtagccgttc tttcaccgga ctggcggacc
      841 tggcgggccg ccgtgtacgc atcagcaacc cgtcgcaggc agatctgatc cgcccatttg
      901 gagctgttcc catacagtcg gagttttctg cggtcgtctc gggcatgcga agcggcaatg
      961 tggactgtgc cattaccggc gccatgtccg gaaacgcgat tggcctgcag gaagttgcgt
     1021 cccatttgca caccagtgcg accacttggg gcttgtctgt ttttggtgcc aatctgggtg
     1081 catggacggc acttccgccg gatatgaaat ctctgatcaa gacggagttg ccaaaactgg
     1141 aggccgcaat ctgggcggat tccgagcgcc aaaccgatga gggtgtggct tgcaataccg
     1201 ggcgcggcag ctgcctgact ggcaagaccg ggctcatgaa agaagttcag accaatgcgg
     1261 ttgacgagag caagcttcgt atttcctttc gcgacagcgt cttgccggcc tgggttcagc
     1321 gttgtggcaa tacctgtgtt cccgtctgga atcggcttct cgctccggtt acgggcatac
     1381 gtgctgaaac acaggcgacc aggccatgat ctgcacccat aagaaggcgg aaacccgcgg
     1441 gattgctggc tcaaagcgcc tagcgctgcc ggatggactg cgttgtgctg gacagtccat
     1501 ctgcaccctc cgggcgattc caatgcaggg atcactgcgc cgcgtgaggt gggccgtcct
     1561 gtcggcggca acgttgttga tcctgatcgt gatggcactt gcgaccacgc tgctctatcg
     1621 cgaccacgca gaagcgcttg agcgcgctcg cacactggta acccgtgtcg cgcaaaacag
     1681 cgaggccgac atcaaccgca atttggtaag cgtggacatg atgctggccg agctgagtat
     1741 ctggatcaca caggagcgca cagaaacacc acctccatct aggggtgtgc agacgcttgg
     1801 agttgaaaaa aacctgtcgc ggcttgttcg cgccgccttg cagcaaaatt tgatgctgcg
     1861 cgacattgcg gtgattgctt ctgacggcag tgcgctgatc ggcgcccgac ccagcacgca
     1921 acgactgggg ctgccgatgc cggctggatt tttggatgcc gtgctgcaac aaccttatgc
     1981 gggactgatg gtcagcgctc ccaccttgaa tcccctcaat tcagagcgcg tactctacct
     2041 ggcccgctca caacgcctgc cagatggcat ggtggtcgct gtcgtggccg aactgcagct
     2101 ctcgctgcta tccgcattgc tgaaccctga agacgatagc cagcgtctga ccgtgacgct
     2161 cgaaaaagag tcggggcaac tactggtgag ttacccgatg gtggatgttc tgatcggaca
     2221 aacgctgcac cccgcgctgg aatccctcaa aggcggcgcg ggggcacagg atatgccagg
     2281 acgattgcag gccgagccgt cagtaacggc tgtgcacccc acgttgtatg cgggcctgct
     2341 gctgtcggtt ggcatgccac tccgatccgc tctggcgcaa tggcagtccc agcggaacat
     2401 cgtccttggc ggcactgcag tcttgttgtt tttaatcctg ggaggcagtt gggccacgtg
     2461 gcgcggcttt tctcgcatgg cggcagcacg tgccgaagtt gcactggcca atacgcgcct
     2521 ccaggatgcc aaccagcaat tggctggttc cctttctctg gtggaagcaa cactggaggc
     2581 tacgggggaa gcggttctgg tgattggcaa tgatggccgc attcagcact acaacgcatt
     2641 actggcggtg gccttgcgga tgccagaggc ggtattgacc agcggtgaca tggcgcaatt
     2701 gcgcaatttg cttctggatc agcttcagaa tcccgcagag taccttgatg gaaatgcgcg
     2761 ggcctatgct tcgcctacgg cggaggtgcg cgaccagctg gttttcaagg atggcagggt
     2821 gtttcaacgg cactcccttc cccagttgct ggatggacag gtcatgggcc gggtttggag
     2881 ttttcaggac gtcacggctt tcaaagaggc tgagcgaaaa ctccagctcg cggctagtgt
     2941 attcagccac gcgcatgagg gaatatccat cacggatgcc gctggcaata tcgttgacgt
     3001 caatgacacg ttcatccgca tcacgggcta cgaacgtgaa gaagtcgtag gcaagaatcc
     3061 ccgcattctg caatcagggc gccagtcgcc agaatactat gcagcgatgt ggagggaatt
     3121 gctagagcag gggcgctggt ccggtgaagt atggaatcgc cgcaaaagtg gagaggtcta
     3181 tgctgaacag ctttctatca gtgccgtgcg tgatgtggac ggcaaagtcc aacactacgt
     3241 ttcgttgttc acggacatca gttccatcaa gca
//
LOCUS       DAOSLL010000558         3273 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_25546_length_3273_cov_3.192356, whole genome shotgun sequence.
ACCESSION   DAOSLL010000558 DAOSLL010000000
VERSION     DAOSLL010000558.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25546_length_3273_cov_3.192356"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="PLB25_18850"
     CDS             <1..664
                     /locus_tag="PLB25_18850"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HOE43665.1"
                     /translation="MIGLRLEGGKPLLRAYSIVSANYEEHLEFLSIKVQDGPLTSRLQ
                     HIKVGDKIVVGKKPTGTLLIDYLLPAKNLYLFGTGTGLAPFMSIVRDPATYEMFEKVI
                     LVHGVRQVAELAYHDYLTLELPDHEFLGDMVSEQLLYYPTVTREPFKNQGRVTTLIET
                     GKLQADLGLPKFDPATDRAMMCGSPALLRDLKVVLEKRGFLEGNTTKPGDFVVERAFV
                     EQ"
     gene            668..1690
                     /locus_tag="PLB25_18855"
     CDS             668..1690
                     /locus_tag="PLB25_18855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HOE43666.1"
                     /translation="MSSHDLYAIGNALVDSEYEVSDAQLLAAGVEKRHMTLIDAARRT
                     ELLRHVKGIHARRTGGGSAGNTVVALAQLGGRAFYSCRVADDELGDFYTKDLLSHGVA
                     TNLTGIRAPTGQTGSCLVMVTPDAERSMSTFLGATAEIDETSLQPHDIRKSKVYYMEG
                     YLAASPTGLDAAIKGRKIAKEAGVALATTLSDMSMINFCRAGLDAMVGNHDTGLLDYL
                     FCNEEEAQVWCDSKDLSLICQHMGQQARVVCLTRSAKGCIVLEGGQSTEVPAAKVKAL
                     DTNGAGDMFAGAFLYAVTHGHSHARAAWLANQCAGRVVSQYGNRLSQDAINALKADFA
                     AHARTS"
     gene            1871..2176
                     /locus_tag="PLB25_18860"
     CDS             1871..2176
                     /locus_tag="PLB25_18860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015420481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-potential iron-sulfur protein"
                     /protein_id="HOE43667.1"
                     /translation="MTNRRVFILQSVIGTSALASGMVMAAAPMVEEKDPAAAGLGYVA
                     DSAKADAKKYPKHAAAQLCSNCSLFQGKAGDAAGGCPLFAGKQVAAKGWCSAYAKKA"
     gene            2314..3036
                     /gene="ubiG"
                     /locus_tag="PLB25_18865"
     CDS             2314..3036
                     /gene="ubiG"
                     /locus_tag="PLB25_18865"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019654774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HOE43668.1"
                     /translation="MNTTINADAAEIAKFSDLAHRWWDADGEFRPLHQINPLRLAWIQ
                     GLCPLAGLNVLDVGCGGGILTDSLARSGATATGIDLSTKALRIAQLHALDVQTQNVQF
                     REISAEAMAQEQPGQFDVVTCMEMLEHVPDPASVVQACAMLTKPGGWVFFSTLNRNPK
                     SFLLAIVGAEYVLNMLPRGTHEYAKFIRPSELASFCRDAGLTVSHSRGLSFNPLTQRY
                     WLNNDTSVNYLLAAQKPVTSQP"
     gene            3051..>3273
                     /locus_tag="PLB25_18870"
     CDS             3051..>3273
                     /locus_tag="PLB25_18870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HOE43669.1"
                     /translation="MFADIQAVLFDLDGTLIDSAPDLGRAVDKMRTVRGLPSLPLLQY
                     RPMAGAGARGMLGVGFGITPEHPDFELLRE"
BASE COUNT          710 a          864 c          941 g          758 t
ORIGIN      
        1 catgatcgga ttgcggctgg aaggcggcaa accgctgctg cgggcttaca gcatcgtcag
       61 tgccaattat gaggagcacc tggagttttt gagcatcaag gttcaagatg ggccgctgac
      121 ctccagactt cagcatatca aggttggtga caagattgtg gttggcaaaa aacccaccgg
      181 cacgctgctg attgattacc tgctgccagc caaaaacctg tatttgtttg gcacagggac
      241 cggtttggcg cctttcatga gcatcgtgcg tgacccggct acctacgaaa tgttcgagaa
      301 agtgattctg gtgcatgggg ttcgccaagt cgctgagctg gcataccatg actacctgac
      361 gctggaactc cccgatcatg agttcttggg cgatatggtc agcgaacagt tgctttacta
      421 ccccaccgta acgcgcgagc ctttcaaaaa tcagggccgg gtgaccacac tgattgaaac
      481 cggcaaattg caggctgacc tgggcttgcc aaagtttgat ccggcaaccg accgcgccat
      541 gatgtgcggc agccctgctt tgttgcgaga cctcaaagtt gttcttgaaa aacggggatt
      601 tctggaaggc aacacgacca agccgggtga ctttgtggtc gaacgcgcct ttgttgagca
      661 gtaagccatg agcagccacg acctctacgc catcggtaac gcgctggtgg actcagaata
      721 cgaagtttca gacgcacaac tgctggcggc tggtgttgaa aagcgccaca tgaccctgat
      781 cgatgctgcg cgtcgcaccg aattgctgcg ccatgtgaag ggcatccatg ccagacgtac
      841 cggtggcgga tcagcaggca acacggtagt ggcactggcg caattgggtg gtcgggcgtt
      901 ttattcttgt cgggtggcag acgacgagct tggggacttt tataccaaag acttgctgtc
      961 gcatggtgtt gcgaccaacc tgactggcat ccgtgcgcca actgggcaaa ccggcagttg
     1021 cttggtgatg gtgacaccgg acgcagagcg cagcatgagc acttttctgg gtgcgactgc
     1081 agaaattgat gagacttcgc tgcaacccca cgacatccgc aagtccaagg tctactacat
     1141 ggagggttac ctggctgcct caccaacggg gctggatgcc gctatcaagg gtcgcaagat
     1201 tgcaaaagaa gcgggtgtgg ctctggccac cacgctgagt gacatgagca tgatcaactt
     1261 ctgccgtgcc ggacttgatg cgatggttgg taaccatgac acaggtttac tggattacct
     1321 tttttgcaat gaagaagaag ctcaggtgtg gtgtgactcg aaagacctgt cgctcatctg
     1381 ccagcacatg ggtcagcagg cgcgtgtagt gtgcctgacg cgcagcgcca agggatgcat
     1441 tgtgctcgaa ggggggcaat caaccgaggt gcctgctgcc aaggtgaaag cgcttgacac
     1501 caatggcgct ggcgacatgt ttgctggtgc ttttctgtat gctgtgacac acggtcacag
     1561 ccatgctcgg gcggcctggc tcgccaatca atgtgcaggc cgggtggttt cccagtatgg
     1621 caatcgactc agtcaggatg cgatcaacgc gctcaaagct gattttgcag cacacgcacg
     1681 cacctcttaa tgcatggcca ggatcggtct tgctggtgtc ctgtcatttg catttataac
     1741 aactgactta tttacgcggg tattcaccta aatagtgact ttccgtagcg tagcgtcgaa
     1801 aatagcgggc ggtggccatt gaacaggttc aacggcttca gagtttttca actactcagg
     1861 aaattttcca atgacaaatc gtcgcgtatt cattttgcaa tcggttatcg gtaccagcgc
     1921 tttggcttct ggcatggtca tggcagcagc acctatggta gaagagaaag atcctgctgc
     1981 tgcgggtttg ggctacgtag ctgactcagc caaagctgac gcaaaaaagt atcccaagca
     2041 tgctgcggct cagttgtgca gcaactgctc cctgttccag ggcaaggctg gtgatgctgc
     2101 cggcggatgt ccgctgtttg ctggcaaaca agttgccgcc aagggctggt gcagcgctta
     2161 tgccaagaag gcctaaactc tctcgcctcc tccccaaagc gttctaaccc agggcgcaaa
     2221 actggctgaa gcccaccttg ttggtgggct ttgctttttc tggaacctga ccacattcag
     2281 gttgttgagt ccaaaatttc cgataatcaa ctgatgaata caacgatcaa cgctgatgct
     2341 gccgaaattg ccaaattttc agacctggct caccgctggt gggatgcaga tggtgagttt
     2401 cggccattgc accagatcaa cccgctgcgc ttggcctgga tacagggcct gtgcccactg
     2461 gctggtctga atgtgctgga tgtcggctgc ggcggcggca tattgacaga ctcgctggcc
     2521 agatcaggtg ccaccgctac gggtatcgac ctgtccacca aggcgctgcg catcgcgcaa
     2581 ctgcatgctc tggatgtcca aacccaaaat gtccagtttc gcgagatcag cgccgaggcc
     2641 atggcgcagg agcaacctgg ccaatttgac gtggtgactt gcatggagat gctggagcat
     2701 gtgcccgacc cagcgtctgt tgtgcaggca tgcgcaatgc tgaccaagcc gggcggctgg
     2761 gtattttttt ccacactcaa tcgcaaccca aaatcatttc tgctcgctat cgtcggggct
     2821 gagtatgtac tgaacatgtt gccgcgtggc acacatgaat acgccaaatt catccgtccc
     2881 agcgaactcg ccagtttttg ccgcgatgcc ggactaaccg tatctcacag ccgggggctg
     2941 tcatttaatc cgttgaccca gcgctactgg ctaaataacg acaccagcgt caattatttg
     3001 ctggctgccc aaaagccggt gacttcgcag ccatgactcc gtttgcgggt ctgtttgccg
     3061 acatacaggc ggtgcttttt gacctggacg gcacgctgat tgatagcgca cccgatctgg
     3121 gtagagcagt tgacaaaatg cgcacggttc gtgggttgcc atctcttcct ttgttgcagt
     3181 atcgtccgat ggctggtgcc ggcgcgcgag gcatgttggg tgtcgggttt gggatcacgc
     3241 ccgaacaccc ggatttcgag ttgctgcggg agg
//
LOCUS       DAOSLL010000559         3260 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_25705_length_3260_cov_5.394696, whole genome shotgun sequence.
ACCESSION   DAOSLL010000559 DAOSLL010000000
VERSION     DAOSLL010000559.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25705_length_3260_cov_5.394696"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="PLB25_18875"
     CDS             <1..300
                     /locus_tag="PLB25_18875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43670.1"
                     /translation="SGTATPIETSVPPALAATSAASSTASQVKMGNVRIEIVGGWGNV
                     IVDGELKGTVPPVLLISLEPGSHDIEIQNPASAIEKRKVAVLPGKTVLVRHSFVK"
     gene            450..812
                     /locus_tag="PLB25_18880"
     CDS             450..812
                     /locus_tag="PLB25_18880"
                     /inference="COORDINATES: protein motif:HMM:NF038027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TssQ family T6SS-associated lipoprotein"
                     /protein_id="HOE43671.1"
                     /translation="MTKPSALPPVRPSDAVLDEPLSSERKLAIAVSSFETGAYSTAMK
                     QLLPLTKDSSLSAPDRLKAIKFLAFSQCLTRAVIACQKTFESAFRLDSDFDLAPAEQG
                     HPLWKNQFQRARQNVKAK"
     gene            1011..2363
                     /gene="tagH"
                     /locus_tag="PLB25_18885"
     CDS             1011..2363
                     /gene="tagH"
                     /locus_tag="PLB25_18885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HOE43672.1"
                     /translation="MRTTGGLRCLFGSGSSGLGNSGSMPEITIEVVSLDSAPVPALSC
                     QFAGLGGTIGRDNGNTLVLRDPHRRVSRLHASVTFPKGLAVITNTSTSLPVTVGTTML
                     ANGQKLPICADDMIEIGPFVLRVLPQQSNQVGDSPLAANESGELAIAEPLSNPPFPPF
                     SAPDEKDPIADLFSDVSSVAKELACKDSTAANLVIPRSTPLFDGMAEIRAAHQPQPQR
                     AFIPNASPSVGASSHANLDLDLVASGGGAGVDALTAAFLQGAGLAVSALPRGITPEVM
                     AVIGRLLRSATAGVIDMLAARAATKSELQASVTLIPAQSDNPLTFLPNAESAMQQMLG
                     QCRPGMMRADEAMQSAFDDLRAHEIGVNAGIRAALTEVLDRFDPFVLEKRLADESLLE
                     SLLPAMRKKKLWDSFLDQYLAIKREAEDDFHAGFGHSFLAAYEQEYAHAKTHKAQSGK
                     "
     gene            2366..3181
                     /locus_tag="PLB25_18890"
     CDS             2366..3181
                     /locus_tag="PLB25_18890"
                     /inference="COORDINATES: protein motif:HMM:NF025053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HOE43673.1"
                     /translation="MSSNKVLRVASLSLTHCGGRDYNEDAVDQCEAFGAVQLFMLADG
                     AGGQGGGDVASKTALETARQVFLGIPVFSPDTLELCMEKADEAIVFKQKSAPRLAHMA
                     STFVALLINAERGQALLGNLGDSRCYIFRGNQILAQSYDHSLVQRFIDAGLYPAAKLR
                     RHPRRNVLYASLGANPERVSPYLTPVPIDLQAGDAVLLCSDGVWELLEDTQLCDLNQS
                     SETLEIWRDRLQAQVAAVMPPDHDNFSAVLVRILPHDLHGPDEEICTVRPLKN"
BASE COUNT          728 a          847 c          918 g          767 t
ORIGIN      
        1 tcgggaacag ccaccccgat tgaaaccagc gtgccgccag cacttgcggc aacttcggcg
       61 gcaagcagta cagcttcaca agtcaaaatg ggcaatgtgc gcatcgaaat tgtgggggga
      121 tggggcaatg tcattgttga tggtgaactc aagggcacag tgccgccggt tctgttgatt
      181 tccctggagc cgggtagtca tgacattgaa atacagaatc cggcatcagc catcgagaag
      241 cgcaaggtcg cggttttacc aggcaagacc gtgctggttc ggcacagttt tgtcaaatag
      301 gtgagtattt ttacaagaga ggtgtttttg tgaagcagtc tttccgacta ttcgtgttcg
      361 tcgtgtcatg tgtttttttc agtggttgtg caagccctcc cagtggccag caagatccgc
      421 acaagccaga tgcgaagccc cttgacgctg tgaccaaacc gtcggcattg cccccggtca
      481 gaccgtctga tgcggttttg gatgagccgt tgtcaagcga aagaaagctt gccattgccg
      541 tgtcaagctt tgaaactggc gcttattcca ccgccatgaa gcaacttttg ccgctcacca
      601 aagacagttc gctcagtgcc cctgaccgat tgaaagccat caagttcctc gctttttcac
      661 agtgcctgac gcgcgcggtc atcgcctgtc aaaaaacgtt cgagagtgcc tttaggctag
      721 attctgattt tgatctggca cccgccgagc agggccaccc cctgtggaaa aatcaatttc
      781 agcgggcacg ccaaaatgtc aaggccaaat gatctgctca attgagagct gttcaatcat
      841 tcagaagatc agtcttcaca tttcctgcga agaatattga tgtgccaaga tgaatcaggc
      901 gcgtatttcg ccatcggcaa agtgcggttg gactcaatcg cttgcaaact tcttcatgat
      961 ctacccaagg acaattgcca ggaggtcaat cggctgaagg tccaacaatc ttgcgcacga
     1021 ccgggggttt gcgttgcctt ttcggttccg gctcgtccgg cttagggaat agcggttcaa
     1081 tgcctgaaat cacaattgaa gtggtgtctc tggactcggc cccggtgccg gcactgtcgt
     1141 gtcaatttgc tggacttggc ggcacgatcg gtcgtgacaa tggcaacaca ttggtgctgc
     1201 gagatccgca ccggcgggtg tcgcgcttgc atgcgtctgt cacatttccg aaaggattgg
     1261 cagtcatcac caataccagc acgtcactgc cagttacggt cggcacaacc atgttggcga
     1321 atgggcaaaa gctgccgatt tgcgcggatg acatgattga gatcgggcca tttgtcttgc
     1381 gcgttcttcc gcagcagtcg aatcaagttg gagacagccc actggcagcc aacgaatccg
     1441 gcgaattggc tattgcagag cctctttcaa acccaccctt tccgccattt tctgctccgg
     1501 acgaaaagga ccccattgcg gatctatttt cggatgtcag ttcggtggct aaagagttgg
     1561 catgcaaaga ttcgaccgct gccaatcttg tgataccaag gagcacaccc ctgttcgatg
     1621 gcatggccga aattcgcgca gcgcatcagc cgcaacctca gcgtgcattc atcccaaatg
     1681 catccccatc ggtcggggca agcagtcatg cgaacttgga cctcgatctt gttgcatctg
     1741 ggggcggtgc aggcgttgat gctctgacag cggcgtttct gcaaggagcg ggacttgcgg
     1801 tcagcgcctt gccaaggggg atcacacctg aagtcatggc ggtaataggc cgcttgctgc
     1861 gctcagctac agcaggggtc attgacatgt tggcggccag ggctgcgacc aaatcggagc
     1921 tgcaagccag cgtcaccctg atccctgccc agtctgacaa tccgctcacg ttcttgccca
     1981 atgccgaatc agcaatgcag caaatgctgg gccagtgtcg gcctggaatg atgcgggccg
     2041 acgaggcgat gcaaagcgct tttgatgacc ttcgtgccca cgaaatcggc gtgaatgcag
     2101 gcatccgggc ggcgctaacg gaggtgttgg atcgatttga tccgtttgtg ctggaaaagc
     2161 ggttagccga tgagtcgttg ctcgaaagcc tgcttcctgc catgcgaaag aagaagctct
     2221 gggatagctt cctggaccag tatttggcta taaagcgcga agctgaagac gattttcatg
     2281 ccggttttgg gcattctttt ttggccgcat acgagcaaga atacgcccac gcgaagacgc
     2341 ataaagcgca gtctggaaaa tagcaatgag ttcaaacaag gtgttgcggg tggcgagtct
     2401 gagtcttacc cattgtggtg gccgagacta caacgaagat gcagttgacc aatgtgaggc
     2461 atttggcgcg gttcagcttt tcatgctggc cgatggtgcc gggggccagg gcggtggtga
     2521 tgtcgcctca aaaactgctt tggaaactgc caggcaggtt tttttgggga ttccggtttt
     2581 ttcgccagac accctcgagc tgtgcatgga aaaggccgat gaggcaatcg ttttcaagca
     2641 aaaaagtgcc ccccggctcg cccacatggc ttccaccttt gtcgcactgc tgatcaatgc
     2701 tgagcggggt caggcgttgt tgggcaacct cggcgattct cgttgttaca tattccgtgg
     2761 gaatcagatt ctggcccaat cctatgacca tagtctggtg caacgtttca ttgatgccgg
     2821 actgtatcca gccgccaagt tgcggcgaca ccctcggcgc aatgtcctct acgcgtcctt
     2881 gggggcaaac ccggagcggg tatcgccata tttgacaccg gtaccgattg acctgcaagc
     2941 tggcgatgct gtgcttcttt gcagtgatgg cgtatgggaa ctgctggagg acacacaact
     3001 ttgtgacctc aatcaaagca gtgaaacact ggagatttgg cgcgaccggt tgcaggcgca
     3061 ggttgctgct gtcatgccgc ccgatcatga caatttcagt gccgtgctgg tcaggatcct
     3121 gccgcatgat ttgcatggtc cagacgaaga aatctgcact gtcagaccat tgaaaaactg
     3181 attgcctcac caaaaaaata cggtttgttg gtgccagagt gaagaagaca cacgatggct
     3241 caggctgcag gtgtttgaca
//
LOCUS       DAOSLL010000560         3256 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_25742_length_3256_cov_4.015308, whole genome shotgun sequence.
ACCESSION   DAOSLL010000560 DAOSLL010000000
VERSION     DAOSLL010000560.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25742_length_3256_cov_4.015308"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="PLB25_18895"
     CDS             <1..388
                     /locus_tag="PLB25_18895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017335347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HOE43674.1"
                     /translation="VAAPQVIDWPAAPPGAWSRVVTPDPRLLFRFSALTFNAHRIHYD
                     RPYAMQEEGYPGLVVHGPLTAFLLMDMVRQQAKQTVKAFSFRGLAPLFDLAAFRLIGV
                     AEGAQVSLQAQGPDGAIAMSASAELA"
     gene            complement(460..915)
                     /locus_tag="PLB25_18900"
     CDS             complement(460..915)
                     /locus_tag="PLB25_18900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43675.1"
                     /translation="MAHARAVGFHGELSMSFNRIEFVGCRPFNLCAGPHSMLAMADFH
                     IVAATSHIAQNTIAKQCSGVAWCCRWWKPSRESCLSWASGFIRIIALLCFPPVSLVLV
                     RSIRLRICSKQNCHSRLLAKGEFFADHFTLYWTGPRRPFAYEHGTALND"
     gene            1001..1879
                     /locus_tag="PLB25_18905"
     CDS             1001..1879
                     /locus_tag="PLB25_18905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HOE43676.1"
                     /translation="MKYKDYYETLGVSRDADLEQIKKAYRKLARTYHPDMSKAADAEA
                     RFKDVAEAYATLKDAGKRAAYDELGHPKAGAEFAPPPQWQQDFATGGQAFEDLDLADL
                     LAAFGQGQRRPGADPGPAKGRDFESAVTITLADARKGVTVSLGLADASGQRTLEVTIP
                     PGVRQDQKLRLRGKGGKGHNGGPDGDIYLHIALAPHAVFRPDGLDLYFDLALTPWEAT
                     LGAAVEVPTLDGPVLLTVPAGTRSGRKLRLRGRGLADGHSDIYAIVHIDIPANITEPE
                     RVLYLELARISNFNPR"
     gene            1901..2254
                     /locus_tag="PLB25_18910"
     CDS             1901..2254
                     /locus_tag="PLB25_18910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007863926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HOE43677.1"
                     /translation="MTQDCPEWRWLDTRETVTLTELSHCCGLSEAELDELVDYCALVP
                     LIPVEQEPTFSAQWVIPLRNASKLRLDFDLDLFTVAILLGNFNRIEMLERQVHSLQAL
                     LPQQVHAGLKFQSSP"
     gene            complement(2246..2470)
                     /locus_tag="PLB25_18915"
     CDS             complement(2246..2470)
                     /locus_tag="PLB25_18915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43678.1"
                     /translation="MLDKLKDGMLWPDIHPPELKFVYAAALKHDGIVGDPAWVWDPGR
                     RGAAVCVRAKKSLPRQEVSVSNPGQGLGSW"
     gene            2717..>3256
                     /locus_tag="PLB25_18920"
     CDS             2717..>3256
                     /locus_tag="PLB25_18920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE43679.1"
                     /translation="MTASSAKSAHPVVELVALTKSYGPGKPAVDGINLRIARGSYCCL
                     LGPSGCGKSTTLRMIAGHESVTAGDILLEDRNITDLPAAQRGTAMMFQSFALFPHLTA
                     LDNVAFSLKMKGVDKATRHKQAAELLERVAMGHLSQRKPAELSGGQQQRVALARALIT
                     QPKVLLLDEPLSALDPFLRI"
BASE COUNT          696 a          980 c          958 g          622 t
ORIGIN      
        1 agtggctgct ccgcaggtga ttgactggcc agcagccccg cccggagcct ggtcgcgcgt
       61 cgtcacgccc gaccccagac tgctgttcag gttttctgcg ctcaccttca acgcgcaccg
      121 catccattac gaccgcccct acgcgatgca ggaggagggt taccccggtc tggtggtgca
      181 tggaccgctg accgcttttc tattgatgga catggtgcgt caacaggcga agcaaacggt
      241 caaggcattt agctttcgcg ggctggcacc cctgtttgac ctggcggcgt ttcggttgat
      301 cggtgtcgca gaaggcgctc aggtttcact gcaagcacag gggccggacg gcgcaatagc
      361 catgagcgcc agtgccgaac tggcttagtc ggcgttggcc gcggcatcgg ctcgcaaaca
      421 ctgccgaacc aaagcggccc gcagtgtcaa gacagttttt cagtcattta aagccgtgcc
      481 gtgttcatat gcaaacggtc ggcgtggccc ggtccaatac agggtgaaat gatctgcaaa
      541 aaactcgccc tttgcgagca ggcgggaatg gcagttttgc tttgaacaga tcctcaaccg
      601 aatgctgcga accagcacca gcgaaacagg cgggaaacac agcaaagcga tgatgcggat
      661 gaagccggat gcccaactaa ggcatgactc ccttgaaggt ttccaccagc gacagcacca
      721 tgcaacgcca gaacattgct ttgcaatggt gttttgggca atgtgggagg tagcggcaac
      781 gatatgaaag tcggccatgg caagcatcga gtgtgggcca gcgcacagat tgaatggtct
      841 gcaaccgaca aactcgattc ggttgaagga cattgaaagc tcaccatgaa aaccaaccgc
      901 ccgggcatgc gccatggcag attcacgccg agcgatctgg ctgatgccac cgcgttgatc
      961 gcacagcctg attgcaggca tctgtgccgg gtctgacatc atgaaataca aggactatta
     1021 cgaaacactg ggtgtctccc gcgatgctga tctggagcaa atcaaaaagg cttatcgaaa
     1081 gctggcacgc acttaccacc cggacatgtc aaaggcagcg gatgccgaag cccgcttcaa
     1141 ggacgtagcc gaagcctatg ccacgctgaa agacgctggc aagcgcgctg cctatgacga
     1201 gctgggccac cccaaagcgg gtgccgaatt tgcaccgccg ccgcaatggc aacaagattt
     1261 tgcaactggt ggtcaggcgt ttgaagacct ggacctggcg gatttgctgg ccgcatttgg
     1321 ccaaggacag cgccgaccag gcgccgatcc ggggcccgcc aaaggtcgcg attttgaaag
     1381 cgctgtcacc atcaccctag ccgatgcccg caagggcgtg acggtcagcc tgggcctggc
     1441 cgacgccagc ggccaacgaa ccctggaagt caccatcccg ccgggcgtgc gacaagatca
     1501 gaaactgcgc ttgcgcggca aaggcggcaa gggccacaac ggcggacccg acggcgacat
     1561 ttacctgcat attgcgctgg caccacatgc agtgttccgt ccagacggac ttgacctgta
     1621 ttttgatctg gccctgaccc cctgggaggc cactttgggc gcagcggtgg aagtacccac
     1681 actggacggg ccggtgttgc ttaccgtgcc agccgggacc cgttccggtc gcaaattgcg
     1741 cctgcgcgga cgcgggctgg ccgacggcca tagcgacatc tatgccatcg ttcacattga
     1801 cattcccgcc aatatcacag aacccgaacg tgtgctgtat ctggaactgg cccgtatctc
     1861 gaacttcaat ccgcgctaaa ccgcacaaag gagaaatcaa atgacacaag attgccctga
     1921 atggcgctgg cttgacacgc gggagaccgt caccctgacc gagttgtcgc attgctgtgg
     1981 cctgagcgag gctgaactcg acgagctggt ggactattgc gcgctggttc ctttgatacc
     2041 agtcgagcag gaacctacct tcagcgcgca atgggtgata ccgctgcgca acgcatccaa
     2101 actacggctg gactttgacc tggatctttt caccgtggcg attctgctcg gcaacttcaa
     2161 ccgcatagag atgcttgaac gtcaagtgca ttccttgcag gccctgttgc cgcaacaagt
     2221 ccatgcgggg ctgaagtttc aatcgtcacc atgagcccaa accctgccca gggttgctga
     2281 cgctgacttc ctgtcttggc aagctctttt tggcgcgcac gcaaactgcc gcacctctgc
     2341 gaccgggatc ccatacccag gccgggtcgc ccacaatgcc gtcgtgcttg agcgcggcgg
     2401 catagacaaa cttgagttcg ggcgggtgga tgtcgggcca gagcatgccg tccttgagct
     2461 tgtccagcac cacctatcgg cagacccgcc gaaatgcgcc caagcctgca catgcggcta
     2521 agcccaggac ttctggctga acttccaggc cacccggcga tcactgcgcg ggcagtagcg
     2581 caccagcggc ggcggggttt ggcaccaaat ctggaccatt gacgcacctt tgagcaccaa
     2641 acgtgtgcga ttttgtacgc aatctgtggt tagcctggta cacaatcctg gcacgcgatt
     2701 tgctgatgcc atcggcatga ccgcttcttc tgccaaatct gcccatcctg ttgttgagct
     2761 ggtggccctg accaaaagtt atgggcccgg caagccagca gtcgacggca tcaatttgcg
     2821 cattgcacgg ggcagctatt gctgtctgct cggcccctcg ggctgtggca aaagcaccac
     2881 cttgcggatg attgccgggc acgagtcggt cactgcgggg gacatcctgc tggaagaccg
     2941 caacatcacc gacttgcccg ctgcgcagcg tggcacagcg atgatgttcc agagttttgc
     3001 gctgtttccg cacctgacag cactggacaa cgtggccttc agcctgaaga tgaagggcgt
     3061 ggacaaggcc acccggcaca agcaggccgc agaattgctg gagcgtgtgg ccatggggca
     3121 cctgagccag cgcaagccgg ccgagctctc gggtggtcag cagcagcgtg tggccctggc
     3181 gcgggcactg atcacgcaac ccaaagtgtt gctgttggac gaacccctgt cggcactgga
     3241 cccgtttttg cgcata
//
LOCUS       DAOSLL010000561         3254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_25761_length_3254_cov_3.802126, whole genome shotgun sequence.
ACCESSION   DAOSLL010000561 DAOSLL010000000
VERSION     DAOSLL010000561.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25761_length_3254_cov_3.802126"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1259
                     /locus_tag="PLB25_18925"
     CDS             <1..1259
                     /locus_tag="PLB25_18925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="type I restriction endonuclease subunit M"
                     /protein_id="HOE43680.1"
                     /translation="VDSSNPKYARMVPVSEIADPKNDYNLNLPRYIDSTAPEDLQDID
                     GHLRGGIPTRDLDAPDNALAAYWQVLPGVRAALFASAGRPGYASLTLPLPEVKPAIFG
                     HAEFTAFNQTATQLFARWRTAATQQLTTFCKGGHPKALIEATAEALLTTFKTAKLLDA
                     YDIYQHLMDYWASVLQDDTYLIAADGWVQGAQPREVKQVKDKNNKFVWPEPHDYLKGK
                     RRFKSDLVPASILVAKYFAPERAAIDALDNLLATLEQQLDDMLEEHSGEDGLLAEVIE
                     GEGDKQKISTKPVKARLKEIGKSTDYTQEEEALQDYAALLDQQAVTKTKLKAAQEALD
                     QKIDAKYPTLTEAEIKTLVVDDKWMAHLSGAVQGELDRVSQTLTGRIRELAERYATPL
                     PQLEREVATLAARVDEHLKRMRATWN"
     gene            1686..2582
                     /locus_tag="PLB25_18930"
     CDS             1686..2582
                     /locus_tag="PLB25_18930"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOE43681.1"
                     /translation="MGRVGVVPSGFQGNISRELARLRIRQDTCSEYIAHQLESGATQQ
                     RIVQAVVGTTRLEFSITALRQFQLAIPPTNCEQSAIATALSDVDALLGGLDSLIAKKQ
                     GLKVAAMQQLITGQTRLPGFSGEWEVKRLGDVVQIRKGQLITSSTLLPGDIPVIAGGK
                     QPAYFQASANRFGRTVTISASGASAGYVALYDEPIFASDCSTISESESYCLDFVFYQL
                     LCKQQAIYKAQTGGAQPHIHAKDLNPLFFSFPALPEQTAIATILSDMDTELTALQARR
                     SKTQALKQAMMQELLTGRIRLI"
     gene            2579..>3254
                     /locus_tag="PLB25_18935"
     CDS             2579..>3254
                     /locus_tag="PLB25_18935"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HOE43682.1"
                     /translation="MSNQTITLKTVNELRLDAAGEPRRYFIASYQRGYRWSGLQVTQL
                     LEDIREFTRRRDPQPDDFYCLQPLVLKVSGGGGYEVVDGQQRLTTLLLILRHFNERLT
                     EKFRQQLFALDYETRENLLDFLDHPTEALANSNIDFFHLHGAIKTIEKWFSDKENEVD
                     EIKSALLNKTKVIWFQLSARDSAVDAFARLNVGKIPLTNDELIRGLFLTGQCQVLPVI
                     QAVSWPG"
BASE COUNT          761 a          951 c          899 g          643 t
ORIGIN      
        1 gcgtggacag cagtaatccg aaatacgccc gcatggtgcc tgtgagcgag attgctgatc
       61 ccaagaatga ctacaacctt aacctgccac gctacatcga cagcaccgcg cccgaagacc
      121 tgcaagacat tgacggccac ctgcgcggcg gcatccccac ccgcgacctg gacgcccccg
      181 acaacgcctt ggccgcctac tggcaggtgc tgcccggtgt gcgggccgct ctgtttgcgt
      241 ctgctggtcg ccctggctat gccagcctga ctctgcccct gcccgaggtc aagcccgcca
      301 tctttggcca cgccgaattc accgccttca accagactgc cacccaactc tttgcccgct
      361 ggcgcactgc tgccacgcag cagctcacca cgttttgcaa gggcggtcac cccaaggcgc
      421 tgatcgaggc caccgctgag gcactgctca ccaccttcaa gaccgccaaa ctgctagatg
      481 cctacgacat ctaccagcac ctgatggact attgggccag cgtgctgcaa gacgacacct
      541 acctgatagc tgccgacggc tgggtgcaag gcgcgcagcc gcgtgaagtg aaacaggtca
      601 aagacaaaaa caacaaattc gtctggcccg agccgcacga ctacctcaaa ggcaaacgcc
      661 gcttcaagtc cgacttggtg cctgccagca tcttggttgc caaatacttc gcccccgagc
      721 gcgccgccat tgatgcgctg gacaacctgc tggccacgct ggagcagcaa ctggacgaca
      781 tgctggaaga acacagcggt gaagacggcc tgctggccga ggtgatagaa ggcgaaggcg
      841 acaagcaaaa aatcagcacc aagcccgtca aggcacggct caaagagatt ggaaaatcta
      901 ccgattacac gcaggaggaa gaggcactgc aagactacgc agccctgctc gaccagcaag
      961 ccgtcaccaa aaccaagctc aaagccgcac aagaggcgct ggaccaaaag atcgacgcca
     1021 aataccccac cctgaccgag gctgagatca aaaccctggt ggtggacgac aagtggatgg
     1081 cccacctgtc tggcgccgtg cagggcgagc tggaccgcgt gtcgcaaacc ctcaccggcc
     1141 gcatccgcga actggccgaa cgctacgcca ccccgctgcc acagcttgag cgagaggtag
     1201 ccacactcgc cgcccgggtg gacgaacacc tcaagcgcat gagggcgaca tggaactgag
     1261 gcggggtgac gctggagttg ctactttgtt agtagctgaa tgcgcacgtt ctacgagggc
     1321 tagagccaga tttggcataa attttgactg gcagaggccg tttgaccggt ttttagcggc
     1381 tgcattgcgg gtgggtgcgg tatgagcacg ggtttgcggg cggggtacaa gcagaccgag
     1441 ttgggggtaa ttccggtcga ttggcacgcg gaagaggttg ccaatttagt agagccaaca
     1501 gcacctattt gttatggcgt tgtccaagtt ggaaagtaca cggaaaacgg cgttcccatt
     1561 gtggcgatta agtttgtaaa ggagattgca catgccccct tcatcgaact gcctttaacc
     1621 tcgaacaacc ttacgcacga agcaggccga aaggcgggga tgttcttatc tcaattaaag
     1681 gaacaattgg gcgcgttggt gttgttccaa gcgggtttca ggggaacatt agccgggaat
     1741 tggcaaggct acgaatccgc caggatacgt gctccgaata catcgcacat cagcttgaat
     1801 ctggtgctac tcaacaacgg attgtccagg cagtagtagg gaccacgcga cttgagtttt
     1861 ctatcacagc gcttcgccag ttccaattgg ccattccacc gaccaactgt gaacaaagcg
     1921 ccatcgcaac agccttgagt gatgtggatg cgctgctggg cgggctggac agcctcatcg
     1981 ccaagaagca gggcctcaaa gtagccgcca tgcagcaact catcaccggc caaacccgcc
     2041 tccccgggtt cagtggggag tgggaggtga agcgattggg ggatgttgtt caaattcgaa
     2101 aagggcagct aatcacgagc agtaccttgt tgccgggtga cattcccgtt atcgcaggcg
     2161 gcaagcagcc cgcttacttc caggcttccg caaatcggtt tgggcgaacc gtcacgatca
     2221 gcgcgtcagg ggcgagcgct ggttatgtcg cgctctacga cgaaccgatt ttcgcgtcag
     2281 attgctctac tatcagtgaa tcggaatcct actgcctcga tttcgtcttc tatcaacttt
     2341 tgtgcaaaca gcaggcgatc tacaaagcgc agactggcgg tgcgcagccg catattcacg
     2401 cgaaggattt gaatccactt ttcttctcat ttcccgcgtt gccagaacaa accgccatcg
     2461 ccaccatcct ctccgatatg gacaccgaac tcaccgccct gcaagcccgc cgcagcaaaa
     2521 cccaagccct caaacaggcc atgatgcagg agttgctgac cggcaggatt agattgatat
     2581 gagcaaccaa acgattaccc tcaagaccgt caacgaactt cggctcgacg ctgcaggtga
     2641 acctcgacgc tacttcattg cgtcctatca gcgaggctac cgctggtctg ggctgcaagt
     2701 gactcaatta ctggaggaca ttcgggaatt tacacgccgt cgagacccgc aacctgatga
     2761 tttctattgc cttcagccct tggttctcaa ggtatctggt ggcggtgggt acgaggtggt
     2821 tgatgggcag cagcgactga cgacccttct tttgatcctt cgtcatttca acgagcggct
     2881 caccgagaag ttcaggcagc agctttttgc gctcgactac gagacccggg aaaacctctt
     2941 ggatttcctt gaccacccga cggaggcgct ggcgaacagc aatatcgatt tctttcacct
     3001 gcatggcgcg atcaagacga ttgagaaatg gtttagtgac aaggaaaacg aggttgacga
     3061 aatcaagtct gccctgctga acaagaccaa ggtgatctgg tttcagctct cggcgaggga
     3121 tagcgcggtt gatgcctttg cccggctcaa tgtgggaaag atacctctga ccaacgacga
     3181 gttgatccgt ggactctttt tgactggaca gtgccaagtt ctccccgtta ttcaggcggt
     3241 ttcttggcca ggcg
//
LOCUS       DAOSLL010000562         3254 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_25764_length_3254_cov_3.464208, whole genome shotgun sequence.
ACCESSION   DAOSLL010000562 DAOSLL010000000
VERSION     DAOSLL010000562.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25764_length_3254_cov_3.464208"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(270..2996)
                     /gene="ppdK"
                     /locus_tag="PLB25_18940"
     CDS             complement(270..2996)
                     /gene="ppdK"
                     /locus_tag="PLB25_18940"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HOE43683.1"
                     /translation="MTSPKKFIYVFGTSRTEGAANMKNLLGGKGANLAEMCRLSIGVP
                     SGFTISTEACTEFTAHGRDAVFKMILSEVQQGVAFVESEMGKKFGDTNDPLLLSVRSG
                     GRASMPGMMDTILNLGLNDEAVEGLARKANNERFAWDSYRRFIQMYGDVVMGLKPLSK
                     EEHDPFEIIIETLKEDKGIKLDTELTAQDLKDLVQRFKSLIHARLGRDFPTDPWEQLW
                     GAVWAVFESWNNERACLYRELNDIPDSWGTAVNVQAMVFGNLGNSSGTGVAFSRDSAT
                     GEDLFNGEFLVNAQGEDVVAGVRTPQQVSLEGSRRWAEMALVHEEVRRTQYPSLEELM
                     PEIYQQLLRAEATLENHFKDMQDMEFTIQEGKLWMLQTRNGKRSGAAMVRIAMEMLQQ
                     GVIDEKAALQRVVPDRLDELLHPIFDPKALATSAPIARGLPASPGAATGQIVFFADEA
                     EAWVKDGKAVILVRQETSPEDLRGMSAAKGILTARGGMTSHAAVVARGMGKCCVSGAG
                     AVRVDLKARTMTIGDTVYNEGDWLSLNGSTGEVYVGKIATKDAELSGDFGALMALTDK
                     YKRLDIRANADSPDDAKVARGFGAAGIGLCRTEHMFFEGDRILAVREMILADNEAGRR
                     KALTKLLPMQRGDFEGLFREMHGLPVIIRLLDPPLHEFLPHDDANQRIMAAELRVPLT
                     KIKLRVEELHEFNPMMGHRGCRLGISYPEITEMQTRAVLEAALNVRAEGSHVKAEIMV
                     PLVGTVREFNAQAEVIRRTAAAVFAERGESIDYLLGTMIETPRGALIADSIATQAEFF
                     SFGTNDLTQMTMGFSRDDAGKFLPDYIKRSIYEHDPFQTIDQKGVGLLVQMAVQKGRS
                     SRPDLEIGVCGEHGGDPASISFFHRAGLDYVSCSPYRVPIARLAAALAAIEG"
BASE COUNT          671 a          963 c          912 g          708 t
ORIGIN      
        1 aaaaaggtaa cgcacacctg tcggcattgc catgtctttt tgggacgttc tgctatcgct
       61 gcggtttgcg cagggacccc gttcatcgtc atccctgcaa tggccaaaaa aaaatgaagt
      121 cttcgcaaaa aatgcgaaga cttcacttct taaagacgct gtgggccggt acgactcagc
      181 ccgggggatc atccatcaat tgatcccttc cgcgattgcc tacactctaa aacaacaccg
      241 cttgaggcag cgttgttgcc tactctcttt tagccttcga tagcggcgag tgcagcggca
      301 agccgagcaa ttggcacgcg gtaaggcgag cagcttacgt aatccaagcc ggcccgatgg
      361 aaaaacgata tggaggccgg atcgccaccg tgctcaccac aaacgccgat ttcaaggtct
      421 gggcgtgaac tgcggccctt ttgcaccgcc atctgcacca gcagcccaac acctttttga
      481 tcgatggtct ggaacgggtc atgttcatag atgctgcgct tgatgtaatc gggcaggaac
      541 ttgcccgcat cgtcgcgcga aaagcccatt gtcatctgcg tcaggtcgtt ggtgccaaac
      601 gagaaaaact cagcctgggt cgcaatgctg tcggcaatca aagcaccgcg cggggtttca
      661 atcatggtgc ccagcaggta gtcgatgctc tcgccacgct cggcaaacac ggcggcagcg
      721 gtgcgccgaa tcacctcggc ctgcgcattg aactcgcgca cggtgccgac cagcggcacc
      781 atgatttcgg ccttgacatg gctgccctcg gcgcgcacgt taagggcggc ttcgagcacg
      841 gcacgggtct gcatttcggt aatttccgga tagctgatgc ccaggcgaca accgcggtgc
      901 cccatcatcg ggttgaactc gtgcaactct tccacccgca gcttgatttt ggtcagtggc
      961 acccgcagtt cggcggccat gatgcgctgg ttggcatcat cgtgcggcaa gaactcgtgc
     1021 agcggcggat cgagcaggcg gatgatgacc ggaaggccat gcatctcacg gaacagccct
     1081 tcaaagtcgc cacgctgcat cggcagcagt ttggtcagcg ccttgcggcg accggcttcg
     1141 ttgtcggcca gaatcatctc gcgcactgcc agaatgcggt cgccttcaaa gaacatgtgt
     1201 tcggtgcggc acaggccaat gccggccgcg ccaaagcccc gcgccacctt ggcatcgtcc
     1261 gggctgtcgg cattggctcg gatgtcgagc cgcttgtatt tgtcggtcag cgccatcaat
     1321 gcgccaaagt cgccactgag ttctgcgtcc ttggtggcga tcttgccaac atagacttcg
     1381 ccggtggagc cattcaggga cagccagtcg ccttcgttat agaccgtgtc gccaatggtc
     1441 atggtgcgcg ccttcagatc gacacgaacg gcaccggcac ccgatacgca gcacttgccc
     1501 atgccacgcg ccactacggc agcgtgcgag gtcatgccgc cgcgggcggt cagaatgccc
     1561 ttggccgcgc tcatgccgcg caaatcctcg ggggatgtct cctggcgcac cagaatgacg
     1621 gccttgccat ccttgaccca ggcttctgcc tcatcggcaa aaaacacgat ctggccggta
     1681 gcagcaccgg gcgaggcggg caaaccgcgg gcaatcggag ctgatgtagc gagtgccttg
     1741 gggtcaaaaa tcgggtgcag caactcgtcc aggcgatccg gcacgacgcg ctgaagcgcg
     1801 gctttttcgt caatcacgcc ttgttgcagc atctccatgg caatccggac catggcagca
     1861 cctgagcgct tgccattgcg ggtttgcagc atccagagct tgccttcctg gatggtgaac
     1921 tccatgtcct gcatgtcctt gaagtgattt tcaagggtgg cttctgcgcg cagcaactgc
     1981 tgatagattt cgggcatcag ttcttcgagc gaggggtact gggtgcgccg cacctcttca
     2041 tgcaccagtg ccatctcggc ccagcggcgt gagccttcaa gcgacacctg ctgcggcgtg
     2101 cggactccgg ccaccacgtc ttcaccctgg gcattgacca gaaactcgcc attgaacaag
     2161 tcttcgccgg tggcggaatc gcgcgagaaa gcgacaccgg tgccgctgct gttgcccagg
     2221 ttgccaaaca ccatggcctg gacgttgacg gctgttcccc acgagtcggg aatgtcgttg
     2281 agttcgcggt acaggcaggc gcgctcgttg ttccaactct caaacacggc ccacactgcg
     2341 ccccagagtt gctcccaggg gtcggtcgga aagtcgcgcc cgagccgggc atgaatcagc
     2401 gacttgaaac gctgcaccag atctttcaga tcttgcgcag tgagttcggt atcgagcttg
     2461 atgcctttgt cctccttgag ggtctcgata atgatctcga acgggtcatg ctcttccttc
     2521 gacagaggtt tcaagcccat caccacgtca ccatacatct ggataaagcg gcgatacgag
     2581 tcccaggcaa agcgttcgtt gttggccttg cgtgccaggc cctcaaccgc ctcgtcgttg
     2641 aggcccagat tcaggatggt gtccatcatg ccgggcattg aggcgcgacc acccgaacga
     2701 actgaaagca acaacggatc attggtgtcg ccaaacttct tacccatctc gctttctaca
     2761 aaagctacac cttgctggac ctcagacagg atcatcttaa acacagcatc acgcccatgc
     2821 gcagtaaatt cggtgcaggc ctcagtgctg atggtgaacc ccgagggcac cccaatgctc
     2881 aagcgacaca tttcggccag attggcacct ttgcctccga gcagattttt catattggca
     2941 gcgccttcgg tgcggctggt gccaaagacg taaatgaatt ttttgggtga ggtcatggtt
     3001 agtagtatcc tgtgttgaaa gtcttctggt ggtaaggtaa atgagattgc ccgtcagtgt
     3061 aacaagcact ttaggccaca aaacgttcca atcattaggg tttacaacta gatatgggac
     3121 tgctcgacgc gtcttggagt ccaaagcgcg gcaggcctga gcgcccgtgg tacgatgatt
     3181 tgtgagacgc tgttgaattt cgttttctgc ctgtcaattc aaacgacttt tgacttctat
     3241 ttttaacttc tgtt
//
LOCUS       DAOSLL010000563         3252 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_25789_length_3252_cov_3.838599, whole genome shotgun sequence.
ACCESSION   DAOSLL010000563 DAOSLL010000000
VERSION     DAOSLL010000563.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3252
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25789_length_3252_cov_3.838599"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="PLB25_18945"
     CDS             complement(<1..387)
                     /locus_tag="PLB25_18945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012348245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE43684.1"
                     /translation="MNFKRTTLFTLMTAAAGLASAGEVKYMLWDSNQLPAYKQCAADF
                     EKKNPGTTIKITQSGWNDYWTAISTGFVSGTAPDVFTNHLAKYPEFAKNEQLVDLTPL
                     IQRDKVDTGAYSNGLFDIWGRNGKQYG"
     gene            complement(449..1204)
                     /locus_tag="PLB25_18950"
     CDS             complement(449..1204)
                     /locus_tag="PLB25_18950"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HOE43685.1"
                     /translation="MESATLWPSEPTLDNFQRVLGFMTQDESRARGGSGAEINFLRSL
                     GNSLIFTFTIVLTQVSFAAMAAYAFARLRFPGRNTLFALFVGSMMVPGVVTFIPNFIL
                     IKELGLLNTMLGMVAPFCLMQGFSVFFLRQFFLSIPRELEEAALLDGASYFYIFIRVV
                     LPLSITPLATIAMLTGINMWNEFFWPFLVAKDEAQQVLPVALQTFKSQTPQGQPDWTG
                     LMAATAITVLPTLLMLVVLGRRVVESVQFSGGK"
     gene            complement(1207..1803)
                     /locus_tag="PLB25_18955"
     CDS             complement(1207..1803)
                     /locus_tag="PLB25_18955"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HOE43686.1"
                     /translation="MEKKLPETLTEIRKIWAHGKPIKLMFQDEARFGRIADVRRCWAP
                     KPIRPLCQGMLTHEYTYAFGAVDVCTGELDSLILPHVNTGCMQLFLNEVSKRHSDEQI
                     VMVIDGAGWHRSEELKAPANIYLLKLPPYAPELNPIEHVWDELREKFFHNRVFKSLDA
                     LEDHLALALKALEDDPNRLESIVSWPWIISALLVSLLN"
     gene            complement(1776..2156)
                     /locus_tag="PLB25_18960"
     CDS             complement(1776..2156)
                     /locus_tag="PLB25_18960"
                     /inference="COORDINATES: protein motif:HMM:NF024980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOE43687.1"
                     /translation="MSLADTAQVIGVSKGWACQLRREFMRGHKAGDPQTPVPGGRKRQ
                     NMSVTQEREFLAPFLESASAGGVLVVGQIKAALDRQLGREVALSSVYNLLHRHNWRKL
                     APDKRHPQSDPLAQQEWKKNSPKR"
     gene            2742..>3252
                     /gene="argA"
                     /locus_tag="PLB25_18965"
     CDS             2742..>3252
                     /gene="argA"
                     /locus_tag="PLB25_18965"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HOE43688.1"
                     /translation="MSSVFNFTFVPWFRSVAPYIHKFRNQTFVVGVCGEAIAAGKLPN
                     LAQDLALIQSMGVKIVLVHGFRPQVNDQLSAKNHTPQYFKGIRITDEIALDCAQEAAG
                     QLRYEIEAAFSQGLPNTPMAGATVRVISGNFLTARPVGIVDGVDFQHSGLVRKIDTAG
                     ITRSLELGAL"
BASE COUNT          732 a          873 c          926 g          721 t
ORIGIN      
        1 gccgtactgt ttgccgttgc ggccccagat gtcgaacagg ccgttggagt atgctcctgt
       61 gtcgaccttg tcgcgctgga tcagcggagt caggtcaacg agttgttcat tttttgcgaa
      121 ctccgggtac ttggccaaat ggttcgtgaa cacgtcgggt gccgtgccag agacaaagcc
      181 ggtagaaatc gcggtccagt agtcgttcca gcccgactgc gtgatcttga tcgtggtgcc
      241 agggtttttc ttttcgaaat cagcggcgca ttgtttgtag gccggcaact ggttagagtc
      301 ccagagcatg tacttgactt cgccggcgct ggcgagtccg gcggcggccg tcatcagagt
      361 gaaaagggtg gtgcgtttga agttcatggg gttgtctcct gcgatggatg gttgagggga
      421 aagtggaaag ataagaggtt ggtgcggcct acttgccgcc gctgaattgc actgactcaa
      481 cgacgcgccg acccagcacc accagcatca gcagcgtagg aagcacagtg attgcggtgg
      541 ctgccatcag gccggtccag tcgggctggc cctgcggtgt ctggctcttg aaggtttgca
      601 gggccaccgg caatacttgt tgggcctcgt ctttggcgac cagaaagggc cagaagaact
      661 cattccacat gttgatgccg gtgagcatcg cgatcgtggc cagcggagta atgctcagtg
      721 gcagcacgac gcgaatgaag atgtagaaat aggaggcgcc atcgagcaac gcggcttcct
      781 ccagttcgcg tggaatcgac agaaaaaact ggcgcaggaa gaacaccgaa aacccctgca
      841 tcaggcagaa cggagccacc atgcccagca tggtgttgag caggccaagt tccttgatca
      901 ggatgaagtt cggaatgaaa gtgactacac caggcaccat cattgagcca acaaatagtg
      961 caaacagcgt attgcgcccc gggaagcgaa gacgtgcaaa agcataggct gccatcgcag
     1021 caaagctcac ttgtgtcaac acaatagtga aggtgaaaat cagcgaattg ccaagagatc
     1081 gcaagaagtt gatttcagcc cccgatccgc cacgtgccct gctttcgtcc tgcgtcatga
     1141 atccgagcac gcgttggaag ttgtccagtg tgggttcgct gggccacaag gtggctgatt
     1201 ccatttctaa ttcaaaagtg aaactaaaag tgctgaaatg atccaaggcc aagagacgat
     1261 agactccagg cgattggggt catcctccaa ggctttgagc gccagggcca agtgatcttc
     1321 caaagcatca agacttttga agacacggtt gtgaaagaat ttctcccgca gctcgtccca
     1381 aacatgttca attggattga gctcgggggc gtacggtggg agtttgagta ggtagatgtt
     1441 ggcaggagct ttgagttcct cactgcgatg ccagccagca ccgtcgatca ccataacgat
     1501 ctgctcatca gaatgacgct tggagacttc attgagaaat agttgcatgc acccggtgtt
     1561 gacgtgtggc aggatcaggc tgtcgagttc gccagtgcaa acatcgacgg caccaaacgc
     1621 gtaggtgtac tcgtgcgtca acatgccctg gcaaagtggg cgtatcggct tgggtgccca
     1681 gcacctgcgc acatcagcaa tgcgtccaaa acgcgcttcg tcctggaaca tcaacttgat
     1741 gggcttgccg tgcgcccaga ttttgcggat ttcggtcagc gtttcgggga gttttttttc
     1801 cactcctgct gagccaaggg gtcactttgc ggatggcgct tgtctggagc gagctttctc
     1861 cagttgtggc ggtgcagcag gttgtagacc gatgacaggg cgacctcgcg gcccaattgt
     1921 ctgtccagcg cagccttgat ctgcccaaca acaagcacgc ctcctgcact ggctgactca
     1981 agaaatgggg ctaaaaattc acgctcctgg gtcacgctca tgttctggcg cttgcgtcca
     2041 cctggaacgg gtgtttgtgg atcacctgct ttgtgaccac gcatgaattc ccgacgcaat
     2101 tggcaggccc aacctttgga gacgccaatg acctgggccg tgtcggcaag gctcatggcg
     2161 taatccagcg gcagcacaac agcttgtgct tgacgcagtt gctccaccgt ctttgcacta
     2221 gcgatggtct cgcgggcatt tcgcaaaact tcagcatctc cgcttgccgg tcgtgccatg
     2281 gcatatctcc atctggattg atggagatat tattacatta atgaattaga aatagaatca
     2341 cacaccacct acagcaacag atatgcttgg cagggattag cggatttcga tggtcagggt
     2401 cgatgcatct tcatcaggca tgctcaacac cagctcacca ggtcagagca ctttcggccg
     2461 cgatgcgagc gatcgaaaag tcgtgaatat ccagcgtcaa tatcttgttc aattcagcgc
     2521 gacacagcac ctgcaagaaa aagggtcacg tcaactactg ccgcctccac cgtcggcaga
     2581 ggcacaggca aacgctgtaa gtacgcttat gctgcggtat ggtgtggctc gccatgtcaa
     2641 aggcccagaa atcagccaac accccagcgc aagggacagc gggctgactg atacactttt
     2701 tgcgtccgga tcaccagcat tcaccccccc ctatcgtccc tatgtcctct gttttcaact
     2761 ttacttttgt cccctggttc cgttcggtgg cgccctacat tcacaagttt cgcaaccaga
     2821 cttttgtggt gggggtgtgc ggcgaggcga ttgcggctgg caagctgccc aatctggccc
     2881 aggacctcgc attgattcaa agcatggggg tgaagattgt gcttgtgcac ggctttcgcc
     2941 cgcaggtcaa tgaccaactc tcagccaaaa accacacccc ccaatacttc aagggcattc
     3001 gcatcaccga tgagatcgcg ttggactgcg cgcaggaggc tgccggccaa ttgcggtacg
     3061 agattgaagc cgcctttagc cagggactgc ccaatacacc catggccggg gccacggtga
     3121 gggtgatttc gggcaacttc ctgacggccc gacccgttgg tattgttgac ggtgttgact
     3181 ttcagcactc gggattggtg cgcaagatag acaccgcagg catcacgcga tcgcttgaac
     3241 tcggcgcact cg
//
LOCUS       DAOSLL010000564         3239 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_25916_length_3239_cov_3.301822, whole genome shotgun sequence.
ACCESSION   DAOSLL010000564 DAOSLL010000000
VERSION     DAOSLL010000564.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3239
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25916_length_3239_cov_3.301822"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            145..558
                     /locus_tag="PLB25_18970"
     CDS             145..558
                     /locus_tag="PLB25_18970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HOE43689.1"
                     /translation="MMKSMQKGFTLIELMIVVAIIGILAAVALPAYQDYTKKAKVSEV
                     VLAASGARTCISEIFQSTGSATMPASVSSCGTSTSTKYAGTVGVSSAGVITVSGASDV
                     AGVSVVLTPTVSGGVIATWVCSGGPLSLMPGSCRG"
     gene            777..2216
                     /locus_tag="PLB25_18975"
     CDS             777..2216
                     /locus_tag="PLB25_18975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43690.1"
                     /translation="MPALLEQARLAGWGAGEQQKLAIYADFFSGQSSIGYQRLMDNAE
                     ARLDFDLFMTAMTYCYKHDRFAEGYQLLKSFELDRAQRLDLSEYYPCAAYAAFNGGGT
                     VHEAAGYLEQALNEGLHTALVVVNAYVIFFEAGLLERLAELNRLIRNNFASDTEAIYT
                     LGNVELGRDYYPEGFRLMEARYHRPETLKWLSPTLIQAARWQRKPLAGKRLFIHGEQG
                     VGDALMMARYLSLLEQQGAKVILECPDSVSPLLAHNFPGCELVPLQVGKEIEQPFDFW
                     TGMMSLPFHFNTTADNVPCTAGYLSVPPEQAAYWHDRVKQLNVGRRPKVGIAWSGNPR
                     HRFDRRRSITFEKLVPHLRAVSQVQFFALQTDLPTMGPAQPVNVSDELLTFADTAALI
                     AEMDLVITVDTSTVHLAGALGKQTWLLLPYRYEWRWSLAGENNNWYDTVKVIRQRTSG
                     NWDELLSDLFQHRLPSWIEDMKTSGTMCV"
     gene            2213..>2794
                     /locus_tag="PLB25_18980"
     CDS             2213..>2794
                     /locus_tag="PLB25_18980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43691.1"
                     /translation="MNMLRTIVNALVPGARANKAAPAILTAEVADAVDRRDTAFLQNW
                     LQSLLRADSKSPAFQSLMEAVRQNDWPKHDIEKLEIFGLFYQGNLTAAFERASKYTKT
                     PNFDPDLFIIAAFSLFHAGQFEDAYRVLESGKPYEALLDSRSDFAIVATLISQAANVP
                     DALKRYIDLAWRLAPTDPVIALNACAIYFESGDM"
     assembly_gap    2795..2804
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2834..>3239
                     /locus_tag="PLB25_18985"
     CDS             2834..>3239
                     /locus_tag="PLB25_18985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43692.1"
                     /translation="MAVVELAQDHYEEGFRLFEERYKMAEAPRYLNSGLFGLPRWQGE
                     DLATKHLLISAEQGLGDTIQMARYLPELHRLGAAGMVMETQPETLTLLQHNFPDIRMV
                     ERKYGIAPDLYFDRWIGAMSLPALLGTSASKLP"
BASE COUNT          690 a          818 c          914 g          807 t
ORIGIN      
        1 tgacaaaaaa agccacgatt cagagatttt tggtctgagt ttcctaggca aacagcaatt
       61 tggcgcgcta agatgcctgg cacggaagct gctgcaagat cagtgtcctc attgtgagtt
      121 ttgtttttca acctgaagga gttaatgatg aagtctatgc aaaagggttt taccctgatc
      181 gaactgatga tcgttgtggc gatcattggt attttggcgg ccgtggcctt gcctgcttat
      241 caggattaca cgaagaaggc caaggtgtct gaagtcgttt tggccgccag cggtgcccgg
      301 acttgtatca gcgagatttt ccaatcgacg ggttcggcca cgatgcctgc gtcggtgagt
      361 tcatgcggaa cctcgacctc gaccaagtac gccggcacgg tgggtgttag ctctgccggc
      421 gtgatcacag ttagcggagc cagtgatgtc gctggtgttt cagtggtgct gactccgacc
      481 gtttctggcg gtgtaatcgc aacttgggta tgctctggtg gcccgttaag cctgatgcca
      541 ggttcttgcc gcggctaata ggtttgaact cctagcaaag gccaccgatg gtggcctttt
      601 ttacatgtgc agttaagttg atgcgcgact ggttcaaacg gattggctct ttgcttgggg
      661 tatctgcgag cagtacggaa ttgccaccgg acttgctgag tcaactgggg attggccaag
      721 cgtcggagct tcgaccgact cttcaatctg cgatggcagg taatccagtc ttttcggtgc
      781 ctgcattgct cgaacaggcg cggctggctg gctggggggc gggtgaacag caaaaactcg
      841 ccatttacgc cgattttttt tctggtcaaa gttccatcgg ttatcaacgc ttgatggaca
      901 acgccgaagc taggcttgac tttgaccttt tcatgacggc aatgacttat tgctacaagc
      961 atgatcgatt tgccgagggc tatcagttgc tcaagagttt tgagctggac agggcgcagc
     1021 ggctcgattt gagcgagtat tacccttgtg ctgcatacgc ggcattcaac ggcggcggta
     1081 ctgtccacga ggccgccggc tatcttgaac aagcgctgaa tgaaggtctg cacaccgcgc
     1141 ttgttgtagt caatgcctat gtgatttttt ttgaagctgg cctgcttgag cgcttggcgg
     1201 agttgaatcg tttgatccga aacaatttcg ccagcgacac cgaagccatt tacaccctcg
     1261 gtaacgtcga actgggtcga gactactacc ccgaggggtt cagattgatg gaggcgcggt
     1321 atcaccgccc tgaaaccttg aaatggctca gccccaccct gattcaggcg gcccggtggc
     1381 agcgcaagcc acttgcgggc aaacgactgt tcattcacgg tgagcaaggt gttggcgacg
     1441 ctttgatgat ggcacggtat ttgtcgttgc tggaacaaca gggtgccaag gtgattctgg
     1501 agtgtccgga ttcggtcagt ccgttgctgg cgcacaattt tccaggctgt gaactggtgc
     1561 ctttgcaggt tggcaaagag attgagcagc cgtttgattt ctggaccggc atgatgagtc
     1621 ttcctttcca tttcaatacc acggcggata acgtcccttg caccgcaggc tacctgtcgg
     1681 tgccgcccga gcaggctgcc tattggcatg atcgggtgaa gcagttaaac gtggggcgaa
     1741 ggccaaaggt tggcattgcc tggtcaggca acccacgcca caggtttgat cgccgccgtt
     1801 ccatcacctt tgagaagtta gtgccgcatc tacgcgctgt ttcacaggtg cagttcttcg
     1861 cattgcagac cgacttgcca acaatgggcc cagcccaacc ggtcaatgtg tccgacgagt
     1921 tgctgacttt tgccgatact gccgcactca tcgccgagat ggatctggtt atcacggtgg
     1981 atacctcgac tgtccatctg gcgggcgcct tgggcaaaca gacctggcta cttttacctt
     2041 atcggtatga gtggcgctgg agtctggccg gggaaaacaa caactggtat gacacggtga
     2101 aagtgatccg gcaaagaact tcgggcaact gggacgaact gctttcagat ctttttcaac
     2161 atcgccttcc ttcttggata gaggacatga agacatccgg gacaatgtgc gtatgaacat
     2221 gcttcgaacc attgtgaatg cgctcgtacc gggtgctcgc gcaaacaagg ctgcaccggc
     2281 gattcttacg gcggaagtgg ctgacgctgt tgaccgtaga gataccgctt ttttgcagaa
     2341 ctggttgcaa tccttgctaa gagccgattc gaaatcgcct gcatttcagt cattgatgga
     2401 agccgtgagg caaaacgact ggccgaagca cgatattgag aagctggaaa ttttcggctt
     2461 gttttatcaa ggaaacctca ccgctgcttt cgaacgtgct tccaagtaca ccaaaacccc
     2521 gaacttcgat cccgacttgt tcatcattgc agcgttctcc ttgtttcacg ctggtcaatt
     2581 cgaggatgct taccgggtac ttgagtctgg aaaaccatac gaggcattgc tcgatagtag
     2641 atctgacttt gccatcgtcg ccacgctgat tagtcaggcg gccaatgttc cagacgcact
     2701 caaacggtac attgatcttg cctggcggct tgcgcccacc gatcccgtca ttgcgctgaa
     2761 cgcctgcgcc atttattttg aatcaggaga catgnnnnnn nnnnactatt cgctggaaca
     2821 aactggcttt gctttggccg tggtggaatt ggcacaagat cattatgagg aaggtttccg
     2881 tctctttgag gagcgctaca agatggccga ggcgccacgg tatctgaaca gcggcctgtt
     2941 tggcctgcca cgctggcagg gggaagacct ggcaaccaag cacttactga tcagcgcaga
     3001 acaagggctg ggggacacca ttcagatggc gcggtatcta cccgagttgc accgccttgg
     3061 cgccgcgggc atggtcatgg aaacgcaacc tgagaccttg accttgctac aacacaattt
     3121 tccggacatc cggatggtgg agcgaaagta cggcattgcc cctgaccttt atttcgaccg
     3181 atggattggt gccatgagtt tgccggcctt gcttggcaca tcggccagca agttacctg
//
LOCUS       DAOSLL010000565         3226 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_26051_length_3226_cov_4.693157, whole genome shotgun sequence.
ACCESSION   DAOSLL010000565 DAOSLL010000000
VERSION     DAOSLL010000565.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3226
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26051_length_3226_cov_4.693157"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /gene="ribH"
                     /locus_tag="PLB25_18990"
     CDS             complement(<1..412)
                     /gene="ribH"
                     /locus_tag="PLB25_18990"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HOE43693.1"
                     /translation="MLIANQGTLDANDHRQNGNRLSIGVVQARFNADITEALAQACHA
                     ELLKLGVLEKNITLVQVPGALEVPVALMAMAKSLKFDALIALGCIIRGETYHFELVAN
                     ESGAGVSRVALDNQLPIANAILTVETLQQAQARQI"
     gene            complement(455..1570)
                     /gene="ribBA"
                     /locus_tag="PLB25_18995"
     CDS             complement(455..1570)
                     /gene="ribBA"
                     /locus_tag="PLB25_18995"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HOE43694.1"
                     /translation="MTILNPVAISPVEDIVADIRAGRMVILVDEEDRENEGDLVMAAE
                     HVSAESVNFMARFGRGLICLTLTEERCERLQLQPMAARNGDKKNTAFTASIEAAEGVT
                     TGISAADRARTVQAAVAKNARPDDLVQPGHVFPLQAVEGGVLMRAGHTEAGCDLAAMA
                     GCTPAAMICEIMNDDGTMARLPDLQVFAAEHGLKIGTIADMIAYRSRVESLVEHMGTR
                     MLNTAAGEFTAHAFKDKTAHGVHLALVRGEWHANEAVLVRVHEPLSVFDALETGRTMH
                     SWSLDASLKKVANEGKGVLVFLNCGETGAQLLAQFDGTATATHGPQRGRMDLRNYGIG
                     AQILRDVGVHKMKLMGTPRRMPSMAGYGLEIVGYVAP"
     gene            complement(1947..2261)
                     /locus_tag="PLB25_19000"
     CDS             complement(1947..2261)
                     /locus_tag="PLB25_19000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43695.1"
                     /translation="MRFYHSAALRSKTNAVLAALETSADQPQHGEAVADLVHELIEAG
                     MDYYFLRALKMAQIGFVAEQSARLGMSGAVKLISSVSRKFIMHMDRQQLLIVASHIRA
                     LT"
     gene            complement(2424..2951)
                     /locus_tag="PLB25_19005"
     CDS             complement(2424..2951)
                     /locus_tag="PLB25_19005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43696.1"
                     /translation="MTAADHATSPGIWLPTMVVPTKTLPIPAWAAGDGEFVLPALLPA
                     SMLLLSAAGGITTSMAMLRDLLARHYQGDVVFLHVCRNPAELACADELQTVTDAFAEL
                     SLCIHFEDTAGPFTPEALQYAVPDLTERSTWMSGPASLTDAVEQFWQQNALAVPLYNI
                     PFLTQAVPARPAPPV"
     gene            complement(2948..>3226)
                     /locus_tag="PLB25_19010"
     CDS             complement(2948..>3226)
                     /locus_tag="PLB25_19010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43697.1"
                     /translation="VRPNPPVQIGEVISDHILITFQNHWCLGAQPHTLQTQGKARRQR
                     HGDSEMESANFPWHSTLIAGKLIDIQCTTVHNTFIHRLSPPSAKRRSR"
BASE COUNT          609 a          912 c         1029 g          676 t
ORIGIN      
        1 caatttgccg cgcctgcgcc tgttgcagcg tctcaaccgt caagatggca ttggcaatgg
       61 gcaactggtt gtccagggcg acccggctga caccggcacc cgattcgttg gccaccagtt
      121 cgaagtggta ggtttctccc cggatgatgc agcccagcgc aatcagcgca tcaaacttga
      181 gcgatttggc catggccatc aaggccaccg gaacctccag cgcaccgggc acctgcacca
      241 gggtgatgtt tttttcgagc accccgagct ttagcaattc ggcgtggcag gcctgtgcca
      301 gcgcctcggt gatgtcggcg ttgaagcgcg cttgcaccac gccaatggaa agtcgattgc
      361 cgttctggcg gtggtcgttg gcatcgagcg tgccctggtt tgcaatcagc atgatggtgt
      421 ggtccgggaa atgggttgaa tcgggagttg ctgcttacgg tgccacgtag ccgacaattt
      481 caaggccgta gcccgccatg ctgggcatgc ggcgtggcgt gcccatcagt ttcattttgt
      541 gcacgccgac atcgcgcagt atctgcgcgc caatgccgta gttgcgcaaa tccatgcggc
      601 cgcgttgcgg gccatgggtg gcggtggcag tgccatcaaa ctgggccagc aactgggcac
      661 cggtttcgcc gcagtttaaa aacaccagca cacccttgcc ctcgttggcg acctttttca
      721 ggctggcatc aaggctccag gaatgcatgg tgcggccggt ttccagtgcg tcgaacaccg
      781 acagcggctc atgcacgcgc accagcacgg cttcattggc gtgccattcg cccctgacca
      841 gcgccagatg cacgccatgc gcggtcttgt ccttgaatgc gtgggcggtg aattcgccag
      901 cagcggtgtt gagcatgcgg gtgcccatgt gctctaccag cgactcgacc cggctgcggt
      961 aggcaatcat gtcggcaatg gtgccgattt tcaggccatg ttctgctgca aatacctgca
     1021 aatcaggcag gcgggccatg gtgccgtcgt cgttcatgat ctcgcaaatc atcgccgctg
     1081 gcgtgcaacc ggccatcgca gccagatcac aaccggcctc ggtgtggcca gcgcgcatca
     1141 gcacgccgcc ttcaaccgct tgcagcggga acacatggcc aggctgcacc aggtcgtcag
     1201 ggcgggcgtt cttggccacg gcggcctgca cggtgcgggc gcggtcggcg gccgaaatgc
     1261 cggtggtcac gccttctgcc gcttcgatcg acgcggtgaa ggcggtgttc ttcttgtcgc
     1321 cattgcgcgc tgccatgggc tgcagttgca gccgctcgca gcgctcttcg gtgagtgtca
     1381 ggcagatcag gccacggcca aagcgcgcca tgaagttgac cgactcggcg ctcacgtgct
     1441 cggcggccat caccaggtcg ccttcatttt cacggtcttc ttcatcgacc agaatcacca
     1501 tgcgaccggc acggatgtcg gccacgatgt cttctacagg cgaaatcgcc acggggttga
     1561 ggattgtcat aagcggtaag ctgccagaaa aaaatagggt tgacgcggtg ccggggtcaa
     1621 catcggtgca ttttcgcatg cccgttgatg gttcaatcca gattgctcag ttggacgcgc
     1681 ccgagtggga cgccggcggc gcggctgggt agggcgaaag ccgcagcttg aacgggccag
     1741 ccctgtggca tggcggcgca ctgcaggggc gagacgacgt tggctactgc ggcgctggaa
     1801 ggagcagcgc ccccaggtgc gttgccagtg gctcaatcgg gtgggtagcg ctgtgaccca
     1861 aagggcgaat cccatcgaag ctaaaaaagt aaatcttgat aagaacttgc gtaggagagc
     1921 aagcggtcaa ccgaggagca acaggatcag gtcaacgccc ggatatggct ggcgacaatc
     1981 aaaagctgct gtctgtccat gtgcatgatg aacttgcggc tgaccgaact gatcaacttg
     2041 accgcacccg acatgcccag tcgggccgat tgttcggcga caaaaccgat ttgcgccatt
     2101 ttgagggcac gcaagaagta gtagtccatg ccggcctcga tcagttcgtg caccagatcg
     2161 gccaccgctt cgccgtgctg cggctggtct gccgacgttt ccagcgctgc cagcaccgca
     2221 tttgttttgc tgcgcaacgc cgctgaatgg tagaaccgca aggacggctc agggactgcg
     2281 gcaccttggc ttttggacgc tgctttggtg actggctttg ctgaggctgt cgtggccgac
     2341 tttgttttgc caggcgcgct ggatttcgcc gtgctggatg cggaggtggt catggtgggg
     2401 tggtgagttg tgaagcggtt tggtcagact ggcggtgcgg gcctggccgg cactgcctgg
     2461 gtcaggaaag gtatgttgta cagcggcacg gccagtgcgt tttgctgcca gaattgttca
     2521 accgcgtctg tcagactggc agggccagac atccaggtgc tgcgctcagt caggtcgggc
     2581 acggcatact gcagggcctc gggggtgaag gggccggccg tgtcttcaaa atggatgcaa
     2641 agagaaagtt ccgcaaaggc atcggtgacg gtttgcagtt cgtcggcaca ggcaagttct
     2701 gccgggttgc ggcaaacatg cagaaacacc acgtccccct ggtaatggcg cgccagcaag
     2761 tcgcgcagca tggccatgct tgtggtgatg ccgcctgcgg cacttaacag cagcatgctg
     2821 gctggcaaca gcgcgggcag tacaaactcg ccatcgcccg cggcccatgc cggtattggc
     2881 agcgtctttg tgggcaccac catggtcggg agccagatgc ccggtgatgt ggcgtggtct
     2941 gcagcagtca tcttgatctc cttttggcag atggagggga gaggcggtga atgaatgtat
     3001 tgtgtacagt tgtgcactga atatcaatca actttcccgc aattagagtc gaatgccaag
     3061 gaaaattggc tgattccatt tctgaatcac catgtcgttg tcgtctcgcc ttgccttgcg
     3121 tctgcaaggt gtgcggctgg gcgcctagac accaatgatt ttgaaatgta ataaggatgt
     3181 ggtcagaaat aacttcccct atttgcaccg gcggatttgg gcgcac
//
LOCUS       DAOSLL010000566         3224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_26074_length_3224_cov_4.576838, whole genome shotgun sequence.
ACCESSION   DAOSLL010000566 DAOSLL010000000
VERSION     DAOSLL010000566.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26074_length_3224_cov_4.576838"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /gene="narH"
                     /locus_tag="PLB25_19015"
     CDS             <1..518
                     /gene="narH"
                     /locus_tag="PLB25_19015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012280.1"
                     /note="with NarGJI catalyzes the reduction of nitrate; the
                     beta subunit is an iron sulfur cluster containing electron
                     transfer subunit; one of 3 nitrate reductases in E. coli
                     and in E. coli is expressed when nitrate levels are high;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HOE43698.1"
                     /translation="KVALPLHPEYRTLPMVWYVPPLSPISAAANAGDIGINGEIPDVK
                     QLRIPVKYLANLLTAGDTMPVERALERMLAMRAYQRGKHVDGKPNLAALQQVQMSTAE
                     VEEMYQVMAIANYEDRFVIPSTHREYAENTFDVRGGCGFSFGNGCSDGASEASLFGGS
                     KKRTIPIKAEV"
     gene            522..1244
                     /gene="narJ"
                     /locus_tag="PLB25_19020"
     CDS             522..1244
                     /gene="narJ"
                     /locus_tag="PLB25_19020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HOE43699.1"
                     /translation="MALFSSNISGASRSLRVLARLLSYPDSLLRGDLNDMRQALVAER
                     ALPTARLAELGALMSTLETGNALDNEADYVEVFDRGRATSLHLFEHVHGDSRDRGPAM
                     IDLAQTYEKAGLFLGPDEMPDYLPVVLEFVSTQPPTEARAFLSEMAHIFNAIFNALQQ
                     RSSMYASVLGALLELSGEKAHPVKIVADEPLDTTWEEPVVFDGCAVKGQTKPDQAQPI
                     HFVKTDAAAHRQKAAASAGVTP"
     gene            1241..1975
                     /gene="narI"
                     /locus_tag="PLB25_19025"
     CDS             1241..1975
                     /gene="narI"
                     /locus_tag="PLB25_19025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HOE43700.1"
                     /translation="MNALDNFLFGVYPYIALAIFLLGSLLRFDRDQYTWKSDSSQMLK
                     AGQLRWGSNLFHIGVLFLFFGHTVGMLTPHFVYEHFISAGNKQLMAMISGGLAGLAGF
                     IGVTILLHRRLSEPRIRINSKTSDIVLLVLLWLQLALGLATVPLSGQHLDGSMMMKLA
                     GWAQHIVTFQPGAVELLSEAGFIFKLHMFLGMSIFVIFPFTRLVHVWSGFASVAYLFR
                     PYQVVRARRLNVPAGNNMPPRPDARV"
     gene            1986..2777
                     /locus_tag="PLB25_19030"
     CDS             1986..2777
                     /locus_tag="PLB25_19030"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HOE43701.1"
                     /translation="MTSFTIEPTLVSRINGIALHAADEALRPDELRQRACSELLRQAA
                     VAQSLLSADDRPSADGVLSDAASSAIEILLERNLLIPEPSDDACQRHYSAHQASYATG
                     ERADVRHILFAVTEGVDVTLLRNRAETCLLNVRCFDGKSQDDPFAKDAKNLSNCPSGA
                     EGGQLGWLIQSDCAPEFAKEIFGSKEIGVLPRLVYSRFGLHVVEILARDPGKNQPFES
                     VRGAVAMALRQKTYVTALRQYLSLLAGNAVLEGVDLDAAASALVQ"
     gene            2788..>3224
                     /locus_tag="PLB25_19035"
     CDS             2788..>3224
                     /locus_tag="PLB25_19035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HOE43702.1"
                     /translation="MKDAVVPDELLARIRRFHDHTFPGVQEQFQTLVSEGQHPSILFI
                     GCSDSRLVPYLLTGTGPGDLFLIRNVGAFVPPYDGSTGYHGTSAAIEFAVLNLKVARV
                     IVCGHSHCGGIRALYEEVNPLAKNLAAWLELGREAALPVQVCP"
BASE COUNT          628 a          957 c          939 g          700 t
ORIGIN      
        1 ggaaggtcgc cctgcccttg caccccgagt accgcacgct gccgatggtc tggtatgtgc
       61 cgccgctgtc gcctatcagt gctgcggcca acgctggcga tattggcatc aacggcgaga
      121 ttccagatgt caagcaactg cgcattccgg tcaagtacct ggccaatctg ctcactgccg
      181 gtgacaccat gccggtggag cgcgccttgg agcggatgct ggccatgcgc gcttaccagc
      241 gcggcaagca tgtcgatggc aagcccaatc tggcggcctt gcagcaggtg cagatgagca
      301 cggccgaggt cgaagagatg taccaggtga tggcgattgc caattacgaa gaccgctttg
      361 tcatccccag cacacaccgt gagtacgccg aaaacacctt cgatgtgcgt ggtggctgtg
      421 gtttcagttt tggcaacggt tgctcagacg gggccagcga agccagcctg tttggcgggt
      481 ctaagaagcg caccatcccg atcaaggcgg aggtttgatt catggcactt ttttcttcca
      541 acatttccgg agcgtcccgc agcttgcggg ttttggcccg gctgctctcg tacccggact
      601 cactactgcg cggtgacctg aacgacatgc gccaggcgtt ggtggccgag cgcgctctac
      661 ccacggcgcg actggccgag cttggcgcgt taatgagtac gctggagact ggcaacgcac
      721 tggacaacga agccgactac gtcgaggtct ttgaccgcgg ccgtgccacc tctttgcatc
      781 tgtttgaaca tgtccatggc gactcgcgcg accgcggtcc ggccatgatc gacctggccc
      841 aaacatacga aaaagccgga ctgtttcttg gccctgatga aatgcccgac tacctgccgg
      901 tagtactgga atttgtctca acccagcccc cgacagaggc cagagcgttt ttgagcgaaa
      961 tggcgcatat cttcaacgcc attttcaatg ccctgcaaca gcgcagcagc atgtatgcca
     1021 gcgttctggg cgccttgctg gagttgagcg gcgagaaagc gcacccggtc aagatcgtcg
     1081 ccgacgagcc actggacacc acctgggaag aaccagtggt gtttgatggc tgcgctgtca
     1141 agggccaaac caagcccgac caggcccaac cgattcactt tgtcaaaacc gacgcagccg
     1201 cccatcgtca aaaggccgcc gcatctgctg gagtcacacc atgaacgcac tcgacaactt
     1261 cctgtttggc gtgtatccct acatcgccct cgccatcttt ctactgggca gcctgctgcg
     1321 ctttgaccgc gatcagtaca cctggaagag cgactcgtcc caaatgctca aggccgggca
     1381 attgcgctgg ggcagcaacc tgtttcacat cggggtgttg ttcctgtttt ttggtcacac
     1441 ggtcggcatg ctgacaccgc actttgttta cgagcacttc atcagtgccg gcaacaaaca
     1501 actcatggcc atgatcagtg gcgggctggc agggctggcc ggtttcatcg gtgtcaccat
     1561 cttgctgcat cgccgcctga gcgagccacg tattcgcatc aactcaaaaa ccagcgatat
     1621 cgtgctgctg gtcttgctgt ggctgcaact ggcgctgggg ctggccaccg tgccgctgtc
     1681 agggcaacat ctggacggca gcatgatgat gaagctggcc ggctgggcac aacacatcgt
     1741 gacctttcag cccggtgccg ttgaattgct gtccgaggct ggcttcatct tcaagttgca
     1801 catgtttctg ggcatgagca tttttgtgat ctttccgttc acccggctgg tgcatgtgtg
     1861 gagcggattt gcttcggttg cttacctgtt caggccctac caggtggtgc gtgctcgccg
     1921 cttgaacgtg cccgctggca acaacatgcc accccgccct gatgcacggg tttaaggact
     1981 tgaccatgac aagcttcaca atcgaaccca cgttggtttc ccgtatcaac ggcatcgccc
     2041 ttcatgcggc agatgaagct ctgcgccctg atgaactgcg ccagcgtgct tgttctgagc
     2101 tgttgcgcca ggcagctgtg gcgcagtctc tgctgtcagc agacgaccgg cccagcgctg
     2161 acggcgtctt gagcgatgcg gccagcagcg ccattgaaat cctgctggaa cgaaacctcc
     2221 taattcctga gccatcagac gacgcttgcc agcgccacta cagcgcgcat caagccagtt
     2281 acgccaccgg cgagcgggcc gatgtacggc atatattgtt tgcagtgacc gaaggtgtgg
     2341 acgtgaccct gctgcgcaac cgcgctgaaa cttgcctgct gaacgtgcgt tgctttgatg
     2401 gcaaaagcca ggatgacccg tttgccaaag atgccaaaaa tctgtccaac tgccccagtg
     2461 gtgctgaagg gggccaactg gggtggctta tccagagcga ctgcgcacca gaatttgcca
     2521 aagaaatttt tggcagcaaa gaaatcggtg tcttgccgcg cctggtgtat agccgctttg
     2581 gcctgcatgt ggtagaaatt cttgcccgcg atcccggcaa gaaccagccg tttgagtcgg
     2641 tgcgtggtgc ggtggccatg gctttgcggc aaaaaaccta tgtgacagcg ctgcggcaat
     2701 accttagctt gttggccggc aacgcggtgc tcgaaggcgt cgacttggac gcggccgctt
     2761 cagccctcgt gcaataggcc ggctgacatg aaagatgccg tcgtacccga cgaactgcta
     2821 gcacgcattc ggcggtttca cgaccacacc tttcctgggg tgcaggagca gtttcagacc
     2881 ctggtgtccg aaggtcagca ccccagcatc ttgttcattg gctgctcgga ctcgcggctg
     2941 gtgccctatc tgctgacagg tactggccca ggcgacttat tcctgattcg taacgtcggc
     3001 gcatttgtgc cgccttacga tggatcaacc ggctaccacg ggacatctgc tgctattgaa
     3061 tttgcggtgc ttaacctcaa ggtggcgcgt gtcattgttt gtgggcacag ccactgcggt
     3121 ggcattcggg cgctttatga agaggtcaac ccactggcca aaaacttggc cgcctggctg
     3181 gaattgggcc gggaggccgc cttgccggtg caggtttgcc caga
//
LOCUS       DAOSLL010000567         3210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_26220_length_3210_cov_3.772742, whole genome shotgun sequence.
ACCESSION   DAOSLL010000567 DAOSLL010000000
VERSION     DAOSLL010000567.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26220_length_3210_cov_3.772742"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1183
                     /locus_tag="PLB25_19040"
     CDS             <1..1183
                     /locus_tag="PLB25_19040"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HOE43703.1"
                     /translation="IYLAGVLADQGQAEEAVDTAELALQQAAEGVNVSVKELSALAQR
                     AAAIALRFEGQPKAWRWLRDALQADPDDATLKLQMARTLVGMGDLDGAVELFDALLQQ
                     QPGHAGILLDRLRARLMLGQLEAARQDADVLLAQAPANPVYQFFAQMAQGKVPKTQPA
                     LLVTTVFDRYATRYDQQMTVAMHYKLPKQVAKMVGEWYPDRKFDLLDLGCGTGLLGRE
                     LKAIDGAFAGQAIDGELIGADLSEQMIIQAQSHGVYANFYKVNLLDALVATPAEHYAV
                     ISALDVFGSVGDLSTVMPGAFRVLTPGGHLVFTFQESDSGDAPYALTTKLNYEYQRDY
                     VQRLLQDSGFTDINMQDVDLRVEEGQAVRGVLVTASKPGEGTEQAPAKAAENAGPDQP
                     A"
     gene            1394..1990
                     /locus_tag="PLB25_19045"
     CDS             1394..1990
                     /locus_tag="PLB25_19045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE43704.1"
                     /translation="MHLSSLEHVERLVAQYLCQQPALNVIDIGSYDVNGSYKAFFSRP
                     NWNYVGVDLAVGPNVDVVLTSPYRLPFASHSADVIVSGQAFEHIEFFWLTWLEMARVL
                     KPGGLIFLLAPSRGYEHRHPQDCWRFYPDGYHALARYGGLELLEVQTDWQPHADEGSA
                     PWGDTVGVFRQRPMSWWLRLRRVAMQHLRYRVLPGYRD"
     gene            complement(2454..>3210)
                     /locus_tag="PLB25_19050"
     CDS             complement(2454..>3210)
                     /locus_tag="PLB25_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43705.1"
                     /translation="ASALQQARARGFVGSDVAHWLWVRRLITLVDHADYYLVRTRRIG
                     WVKRHFEVTGNWPLPGKAGLLCTFHWGAGMWSLPHVADAGLKVHALVAALDGAHFAGR
                     RVLHAYAKARTAIVAKTLGCATLDVSSSLLPALRALKNNEQLLAVVDVPADQVSTSQS
                     IELLGLRASVPRALLRLAVQQHIPVTLFLVGLNTANGRRFLRLRQLGIYADLDNLIRD
                     VFMVLESAIAESPAAWHFWGEAERFFDAEPLQE"
BASE COUNT          683 a          933 c          937 g          657 t
ORIGIN      
        1 gatttatctg gccggcgttc tggctgacca gggacaagcc gaagaagcag tcgacacggc
       61 cgagctagcg ctgcagcagg ccgctgaagg ggtgaatgtc tctgtcaagg agttgtccgc
      121 tctggcgcag cgggccgctg ccattgcgct gcgtttcgaa ggccaaccca aagcctggcg
      181 ctggctgcgc gatgccttgc aggcggaccc cgatgatgcc acgctgaagc tccagatggc
      241 tcgcaccctg gtcggcatgg gcgatctgga tggggcggtt gaactgtttg acgcattgct
      301 gcagcaacaa cccggccatg ccgggatttt gctggaccgc ctgcgtgccc ggctgatgct
      361 gggccagctt gaggcggcca ggcaagatgc cgatgtgctg ctggcccagg caccggcaaa
      421 tccggtgtac cagttttttg cccaaatggc gcagggcaag gtgcccaaaa cacagccagc
      481 gctgcttgtc acgacagtgt ttgaccgcta tgccacccgc tatgaccagc aaatgaccgt
      541 tgccatgcat tacaagctgc caaagcaggt ggcaaagatg gtgggcgagt ggtacccgga
      601 ccgaaagttt gacctgcttg acttgggctg tggcacaggg ctgctggggc gggaactcaa
      661 agccattgac ggtgcgttcg ccggacaggc cattgacggt gaactgatcg gtgcggacct
      721 gtccgagcag atgatcattc aggctcaatc ccatggggtg tatgccaatt tttacaaggt
      781 caacctgctc gacgcgctgg tggccacccc ggccgagcat tacgcggtca tcagcgcgct
      841 ggacgtgttc ggttctgtgg gcgacctgtc aaccgtcatg cccggtgctt ttcgcgtgtt
      901 gacaccgggt ggccacctgg tgtttacatt tcaggagagt gacagcggtg acgcccccta
      961 tgcgctgacg acaaagctca actatgagta ccagcgggat tatgtacaac ggcttttgca
     1021 ggacagcggt ttcaccgaca tcaacatgca agacgttgac ctgcgcgttg aggaaggcca
     1081 ggcagtcagg ggtgtgctgg tgactgccag caagcctggc gaaggtaccg aacaggcccc
     1141 tgccaaagcg gccgaaaatg ccgggcctga tcaacctgcc taaggtgaaa cagccatggg
     1201 taacgcagca gcaagcgtgc tatccggggc tgatttccgt cggttccaga taagggcaat
     1261 ttgggctgca gcggcagagt tgtttcatcc tttcggatgg ccgtgaatgc acgtcaattt
     1321 ggcgacttgc caactggcca gtcgggttga ccactgggta ctggtcattt aacctttatt
     1381 ttggacataa cttgtgcatt tgagctctct ggaacatgtt gaaaggctgg tggcgcagta
     1441 tctgtgccag caaccggcct tgaacgtcat cgacatcggc agctacgatg tgaacggcag
     1501 ctacaaggcc ttcttttccc gccccaactg gaactatgtc ggcgttgacc tggcagtcgg
     1561 ccccaatgtg gatgtggtac tgacatcgcc ttaccgtctg ccgtttgcca gccattcggc
     1621 cgacgtgatc gtgtcggggc aggcgtttga gcacatcgag tttttctggc tgacctggct
     1681 tgagatggcc agggtgctca aacccggtgg attgattttt ctgctcgcac cctcgcgcgg
     1741 ttatgaacat cggcatcccc aggattgttg gcgcttttac ccggatggct atcatgcgct
     1801 ggcccgctac ggtggccttg aactgcttga agtgcaaacc gactggcaac cgcacgccga
     1861 cgagggcagt gcgccctggg gcgatacggt aggtgtattc cggcagcggc ccatgtcgtg
     1921 gtggttgcga ttgcgccgcg ttgccatgca gcatttgcgt taccgggtgc tgccgggcta
     1981 ccgtgattag tcactaaacg aggctttgca atttccggga agaaatcaag aaagtcatga
     2041 agtcattcgt cgcgagggcg cgactcccac aaattcaaaa caattcaact gttctgtggg
     2101 agtcgcgccc tcgcgacgaa ggctgttcga ttcccgctcg tccggctcag gaggcgcgat
     2161 ttctcctgct gaacgtcaaa actggccgcc gctcaatgcg cgataggtgc agtgattttg
     2221 gggtcggatc ccaattgcgt cgacacgcta tcatcacaaa aaacgccaga aagcaattgg
     2281 gctcagaccc caatatcacg gcccaacgcc caaaacctat caaaaacaat agctgacagc
     2341 gcatattgga cgcgcactac aggccttttg tccatgaatt tgaacccaaa gcacagcgcc
     2401 ggtaacagcg gtcgccgtct gtggggtgcc ctcttggtgc cggttatttg acatcactcc
     2461 tgcaaaggct ctgcgtcaaa aaatcgctcg gcctctcccc agaagtgcca cgccgctggg
     2521 ctttcggcaa tagcggactc cagcaccata aatacatcgc gaatcaggtt gtccaaatca
     2581 gcgtagatgc ccaattgccg cagtcgcaaa aaacgtcgtc cattggccgt attgagccca
     2641 accaaaaaca gagttaccgg tatgtgctgc tgcaccgcca gccgaagcaa cgccctgggc
     2701 acacttgccc gcagccccag cagttcaatg gactgactgg tgctgacctg atccgctggc
     2761 acatccacca cggccagcag ttgttcgtta ttcttgagtg cccgcaaggc tggcaacagg
     2821 ctgctgctga catcgagcgt ggcacagccc agggtcttgg caacaatggc ggtgcgcgct
     2881 ttggcgtagg catgcaaaac acgccgaccg gcaaaatggg caccatccag tgccgccacc
     2941 agcgcatgca ctttcagccc agcgtcagct acatgtggta aagaccacat gccggccccc
     3001 caatgaaaag tgcaaagcaa gccagccttg ccgggcaacg gccagttgcc agtgacctca
     3061 aaatgccgct tgacccagcc aatgcgccgg gttcgcacca aatagtagtc ggcgtggtca
     3121 accagtgtga tcaatctacg cacccatagc cagtgcgcca catctgagcc cacaaacccc
     3181 cgggcccgag cctgctgcaa ggcagacgcg
//
LOCUS       DAOSLL010000568         3197 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_26344_length_3197_cov_4.711967, whole genome shotgun sequence.
ACCESSION   DAOSLL010000568 DAOSLL010000000
VERSION     DAOSLL010000568.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3197)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3197
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26344_length_3197_cov_4.711967"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /gene="argS"
                     /locus_tag="PLB25_19055"
     CDS             <1..1240
                     /gene="argS"
                     /locus_tag="PLB25_19055"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HOE43706.1"
                     /translation="TQGWQVYREFYYNDAGVQISTLASSTQLRAQGFKPGDDCWPVNP
                     DDPAAKNFYNGEYIADIAADFLAGKTVRSDDRSFTASGDVEDLDSIRQFAVAYLRREQ
                     DLDLQAFAVKFDNYYLESSLYTSGRVDAAVKMLQDAGKTYRQDGALWLRSTDYGDDKD
                     RVMCKQDGSYTYFVPDVAYHIAKWERGFQKVINIQGTDHHGTIARVRAGLQAANVGIP
                     QGFPDYVLHTMVRVVRGGEEVKISKRVGSYVTLRDLIEWTSKDAVRFFLLSRKPDTEY
                     TFDVDLAVAKNNDNPVYYVQYAHARICSVLTAWGGDVSSLAQVELAPLASAQAQALML
                     LLARYPEMLCAAADGFAPHDVTFYLRELAACYHSYYDAERILVDDEAVRLARLALVAA
                     TAQVLHNGLAVLGVSAPQKM"
     gene            1273..1971
                     /locus_tag="PLB25_19060"
     CDS             1273..1971
                     /locus_tag="PLB25_19060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HOE43707.1"
                     /translation="MRQQRGGTFLGFVLGMVIGLAAALSVAVYVTKVPVPFVSKALSR
                     DPDQDANEQQKNKGWNPNAPLYGKNPAKPTSVAEQGVNAASEPAEVASRPSVSKPVVS
                     KPVAASTPAASATKAELKPAVTADPIGDLVKARATAAAASKAAAPAVEAAADSFIYFV
                     QVGAFRTAEDAESQRAKLLLAGIETKVSEREQSGRMVHRVRLGPFDNRDAAEKAKARL
                     DATGVETALVRVQR"
     gene            2032..2685
                     /locus_tag="PLB25_19065"
     CDS             2032..2685
                     /locus_tag="PLB25_19065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HOE43708.1"
                     /translation="MKRRAFFSGAASLAAAGAALSPLALMAQAKLPKVGVDYVKLNQA
                     APVEAPAGKIEVIEFFWYSCPHCSAFEPVLAEWVKRLPKDVAFRRLPIAFRDDYVPQQ
                     HLYFTLEAMGLLDKFHARVFAAIHGERQNLVKAEAITEWMVQQGVDRAKFQASFSSFS
                     TSTKVRRATQLMNAYNIEGVPALGVAGRFYTDGSLAKSMDAALSVTDFLIAEVRAKR"
     gene            2789..>3197
                     /gene="lptA"
                     /locus_tag="PLB25_19070"
     CDS             2789..>3197
                     /gene="lptA"
                     /locus_tag="PLB25_19070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HOE43709.1"
                     /translation="MKKPFASWLVLCLLAFSATTVLAELADRSKPMNVEADALRYDDA
                     RKVSVFTGRVLLTKGTIQIRGERIEVRQDPQGNQFGLVTALPGKLAFFRQKREGVDEF
                     VEGEGETIDYDGRTDTVKLTGKAQLRRLQGATLN"
BASE COUNT          668 a          921 c          969 g          639 t
ORIGIN      
        1 cacccagggc tggcaggtct atcgggagtt ttattacaac gatgccggtg tccagatcag
       61 cacgctggcc agcagcaccc agttgcgggc gcaaggtttc aagccgggcg acgactgctg
      121 gccagtcaac cccgacgacc ctgctgccaa gaacttctac aacggcgaat acatcgccga
      181 catcgccgca gattttctgg ccggaaaaac ggttcgctcg gatgaccgtt cgttcaccgc
      241 ctctggcgat gttgaagacc ttgacagcat tcgccagttt gccgtggcct acctgcgccg
      301 cgagcaagac ctggacctgc aggcctttgc cgtcaagttt gacaactatt acctggagag
      361 cagcctgtac accagcgggc gggtcgatgc tgccgtcaag atgctgcagg acgctggcaa
      421 gacctaccgg caagacggcg cactgtggct cagatcgacc gactatggcg atgacaaaga
      481 ccgcgtcatg tgcaaacagg acggcagcta cacctacttt gtgccggacg tggcctacca
      541 catcgccaag tgggagcgcg gttttcagaa ggtcatcaac atccagggca ctgatcatca
      601 tggcacgatt gcccgcgtgc gggctggcct gcaagccgcc aatgtgggca ttccgcaagg
      661 tttccccgac tatgtgctgc acaccatggt gcgggtggta cgtggcggtg aagaggtcaa
      721 gatatccaag cgtgtcggca gttatgtgac gctgcgcgac ctgatcgagt ggaccagcaa
      781 ggacgcggtg cgttttttcc tgctcagccg caagcccgac accgaataca cctttgatgt
      841 ggatctggcg gtggccaaaa acaacgacaa cccggtctat tacgttcagt atgcccatgc
      901 gcggatttgc tcggtgctga cggcctgggg tggcgatgtc agctcgctgg cccaagttga
      961 actggcgccg ctggccagtg cgcaagcgca ggccctgatg ctgctgctgg ccaggtaccc
     1021 cgagatgctg tgcgccgcag cggatggttt tgccccgcac gatgtgacct tttacctgcg
     1081 cgaactggct gcctgttacc acagctatta cgatgcagaa cgtattttgg tcgatgacga
     1141 agcggtcagg ctggcacggc tggcgctggt cgccgccact gcgcaggtgt tgcacaatgg
     1201 cctggcagtg ctgggcgtga gtgccccgca aaaaatgtaa ccctaaccgg tcaaaggcga
     1261 caaaggcagc acatgagaca acaacgcggt ggaacttttt tgggctttgt cctgggcatg
     1321 gtgatcggtt tggcggcggc gctgtcggta gccgtctacg tcaccaaggt gccagtaccc
     1381 ttcgtcagca aggccctgag ccgcgaccca gaccaggacg ctaacgagca acagaaaaac
     1441 aagggctgga atccgaacgc cccgctgtat ggcaaaaacc cggccaaacc cacttctgta
     1501 gccgaacagg gggtcaacgc tgcctccgaa cctgccgagg tagcatccag gcccagtgtc
     1561 agcaaacccg ttgtcagcaa gcctgtggcg gcttcaacac ctgctgccag tgcgaccaaa
     1621 gccgaactca agccagccgt cacggctgac ccgatcggcg acctggtcaa ggcacgcgcc
     1681 acggcggctg cagccagcaa ggcggcagcg cctgctgtcg aagcagcggc tgactcgttc
     1741 atctattttg ttcaagtagg tgctttccgg accgctgaag atgccgagag ccagcgtgcc
     1801 aagctgttgc tggcaggcat tgagaccaag gtgtcggagc gtgagcagtc gggccgtatg
     1861 gttcaccggg tgcgcctggg gccttttgac aatcgcgatg cggcagaaaa ggccaaagcc
     1921 cggctcgatg ccaccggcgt tgaaactgcc ttggtgcggg tgcagcgttg aacttttaga
     1981 ttggcgggcg ctctgacgct ttttttgtga acacccctgg agaatcgaat gatgaaacgt
     2041 cgcgcatttt tttctggcgc tgccagcctg gctgctgccg gggcggcact gtcgccactg
     2101 gcgctgatgg cgcaagccaa gctgccaaag gtcggcgttg actatgtcaa gctcaatcag
     2161 gccgcgccag tcgaagcacc ggcgggcaag atcgaggtga ttgagttttt ttggtatagc
     2221 tgcccacatt gcagtgcttt tgagccggtc ttggcagaat gggtcaaacg gctgcccaaa
     2281 gatgtggcgt tcaggcgctt gccaattgct tttcgcgacg actatgtgcc acagcagcat
     2341 ttgtacttta cgctggaggc catgggcctg ctggacaagt ttcacgcacg ggtgtttgcg
     2401 gcaattcacg gcgagcggca aaacctggtc aaagccgagg cgataaccga gtggatggtg
     2461 cagcagggcg tggaccgcgc caagtttcag gccagcttca gttccttttc caccagcacc
     2521 aaggtccgcc gtgcaacaca actgatgaac gcctacaaca ttgaaggcgt gcccgcgcta
     2581 ggggtggcag ggcgtttcta cactgacggc tcgttggcca agagcatgga cgcggcactt
     2641 tctgtcacag attttttgat agccgaagtc cgcgccaaac gatagttggt ttttcacctc
     2701 tcgggtgacg ggaatggctg gctgttcaac tgcaacgggc actggcccgc tacaatgcgg
     2761 cacttaatca tgcaagattc gtgcctttat gaaaaaacct tttgcctcat ggcttgttct
     2821 gtgcttgctg gccttctcgg ccacaaccgt tttggccgag ctagccgacc gcagcaaacc
     2881 aatgaatgtc gaggctgatg cgctgcgcta tgacgatgcc cgcaaggtca gcgtattcac
     2941 tggtcgggtg ctgctgacca aaggcacgat ccagattcgc ggcgagcgca tcgaagtgcg
     3001 acaagacccg cagggcaacc agtttggcct ggtcactgcg ctgccgggca agctggcgtt
     3061 tttcaggcaa aagcgtgaag gtgttgacga atttgtcgaa ggcgaaggcg aaaccattga
     3121 ctacgatggc cgcaccgata ccgtcaagct caccggcaaa gcccaattgc gccgcctgca
     3181 gggagccacc ctgaacg
//
LOCUS       DAOSLL010000569         3196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_26355_length_3196_cov_4.202802, whole genome shotgun sequence.
ACCESSION   DAOSLL010000569 DAOSLL010000000
VERSION     DAOSLL010000569.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26355_length_3196_cov_4.202802"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            222..533
                     /locus_tag="PLB25_19075"
     CDS             222..533
                     /locus_tag="PLB25_19075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HOE43710.1"
                     /translation="MSESIQLSKDKLAQDLHQGIADAEELLRLTADQVGESATELRGR
                     MRDRLSRAKAELAHLQEVTVAKAKAAGHATDEFVHENPWKSIGIAAGLGLVVGLLISR
                     R"
     gene            545..937
                     /locus_tag="PLB25_19080"
     CDS             545..937
                     /locus_tag="PLB25_19080"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HOE43711.1"
                     /translation="MAEATPQSGLLEQLRQRIDAGLSLLQVRLALLGTELEIEKQRLL
                     AGFFWGVAALISLTLALVMFSGLVILLLKDGYRLAALGVLAVAFLAAAAGLQRVASLK
                     LQAGSNLFRASLSELDRDRAALKPHPPP"
     gene            934..1236
                     /locus_tag="PLB25_19085"
     CDS             934..1236
                     /locus_tag="PLB25_19085"
                     /inference="COORDINATES: protein motif:HMM:NF025363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK family protein"
                     /protein_id="HOE43712.1"
                     /translation="MSRADPQLRQQLLLIRSTELRYQLRGSLDSLQRPAAWVDQTRSS
                     LTWLYQHPLVPLAGLLLLVLRKPRRVITWSGKLWWLWKTAKQLQRWHASVLALLPR"
     gene            1321..2403
                     /gene="nagZ"
                     /locus_tag="PLB25_19090"
     CDS             1321..2403
                     /gene="nagZ"
                     /locus_tag="PLB25_19090"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013801001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HOE43713.1"
                     /translation="MSFHAPLIIDIAGPALTKIDRLRLAHPLVGGLILFARNWQDRQQ
                     LSQLCRDIKKLRHDLLICVDHEGGRVQRFRTDGFTHLPPMQALGNLWRGDGNGRAGEG
                     AMRATAAATACGYVLGAELRACGVDLSFAPVLDLDWAASGVIGDRAFDADARVVSLLA
                     KSLMHGMLHSGMANCGKHFPGHGFVKADSHTDVPVDKRSLKAILADDAAPYRWLGGSL
                     ASVMPAHVIYPKVDARPAGFSDKWLGDILRGQLKFTGAIFSDDLSMAGARVIDGQPVS
                     TTQAAVLALNAGCDMVLLCNQSLGVGEELDALINGLAQAQLKGQWLPRQACEVRRLAL
                     LPASAAMPWDDLMVQPGYIQALDLLP"
     gene            complement(2453..2680)
                     /locus_tag="PLB25_19095"
     CDS             complement(2453..2680)
                     /locus_tag="PLB25_19095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007837035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43714.1"
                     /translation="MARQGIWAPKVAALIKGGNSSAAIAQIKVAPSVKDLQDLRKLLS
                     VGRLLAAHPNVDQATLDMIAALSAPRLHRSP"
     gene            2843..3193
                     /locus_tag="PLB25_19100"
     CDS             2843..3193
                     /locus_tag="PLB25_19100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor Sui1"
                     /protein_id="HOE43715.1"
                     /translation="MKAKPTHGGLVYSTDSGRTCPLCRQAVSLCQCSAAKARPASDGI
                     VRVSRETKGRAGKGVTLVKGLALDDAALSALGRLLKTACGSGGTVKDGVIEVQGDHCE
                     RVLEMLKARGLVVK"
BASE COUNT          602 a          912 c          998 g          684 t
ORIGIN      
        1 tataaaataa gcgctggcag cttctgaatt tatagcgttt tatgaccctg acggcaacat
       61 ctgatggcgc tgtggcattg aatgcgccag gtgactgtcg ggtcgcgatt accgagcgtt
      121 gtgaattccc catgctctat gccgccatgc gctctctgca acgcccgaac atgaccgaca
      181 aaggctaaac tcgcttgcct taacccacca ggagattgaa catgagcgaa tccatccaac
      241 tcagcaaaga caaacttgca caagacttgc atcaaggcat tgctgatgcc gaggaattgc
      301 tgcgcctgac cgctgatcag gtcggcgaaa gtgccacgga attgcgtggc cgcatgcgtg
      361 atcggctcag ccgtgccaag gctgaattgg ctcacttgca agaagtgacg gtggccaagg
      421 ccaaagccgc cggccatgcc accgatgagt ttgtgcacga gaacccctgg aaatccattg
      481 gcatcgccgc tggccttggt ttggtggtcg ggctgctgat cagccgccgc tgacactgcc
      541 tgcaatggcc gaggcaacgc cccagagcgg tctgcttgag caactgcgac agcgcattga
      601 tgccggtttg tcgctgttgc aagttcggct ggccctgctg ggcaccgagc ttgagattga
      661 aaagcagcgc ttgctggctg gttttttttg gggtgttgcc gccttgatca gtttgacgct
      721 ggcactggtc atgttcagcg gccttgtcat cctgttgttg aaagacggtt atcggctggc
      781 tgcgctgggt gtgctggcgg tggctttttt ggccgctgcg gcaggcttgc agcgcgtggc
      841 cagcctgaag ctccaggccg gttccaatct gtttcgcgcc agcctgtccg agcttgaccg
      901 cgaccgtgcg gcactcaaac cacacccgcc cccatgagcc gagccgatcc gcaactgcgc
      961 cagcagttgt tgctgattcg cagcaccgaa ctgcgctacc aattgcgtgg cagcctggac
     1021 agcctgcagc gccctgcggc ctgggttgac caaacccgca gcagtctgac ctggctttac
     1081 cagcaccccc tggtgcccct ggctggcttg ctgttgctgg tgctgcgcaa gcccagaagg
     1141 gtcatcacat ggagcggcaa attgtggtgg ctatggaaaa ctgccaaaca gttgcagcgc
     1201 tggcacgcca gtgtgctggc tttgctgccg cgttgaacgg ccgcgcctct tggtccatgg
     1261 ttggcatcgg caccgcgtcg ggcaacttca ggaccatttg cttcttcaga gaaaaacacc
     1321 atgagttttc atgccccgct gatcatcgac attgctggcc cggcactgac caaaattgat
     1381 cgcctgcgcc tcgcgcaccc gctggtgggt ggcttgatcc tgtttgcccg caactggcaa
     1441 gaccgtcagc aattgagcca actgtgtcgt gacatcaaaa agttgcgcca cgacctgctg
     1501 atttgcgttg atcacgaggg cggcagggta cagcgcttca gaaccgatgg cttcacccac
     1561 ctgccgccaa tgcaggcgct gggtaatctg tggcgcggcg atggcaatgg ccgtgccgga
     1621 gagggtgcca tgcgcgccac tgcggccgcc accgcctgcg gctatgtgct gggtgccgaa
     1681 ctgcgtgcct gcggtgttga tttgagcttt gccccggtgc tggacctgga ctgggcggcc
     1741 agcggcgtga ttggtgaccg ggcttttgat gccgatgcgc gcgtcgtcag cctgctggcc
     1801 aagagcctga tgcatggcat gctgcacagc ggcatggcca actgtggcaa gcattttcca
     1861 gggcatggct ttgtcaaggc cgattcgcac accgatgttc cagtggacaa gcgcagtctg
     1921 aaggccatat tggccgacga cgcagcaccg taccgctggc ttggtggcag cctggccagc
     1981 gtcatgcctg cccatgtgat ttaccccaag gtggacgcgc gaccggccgg cttttccgac
     2041 aaatggctgg gtgacatcct gcgcggccaa ctcaaattta ccggtgccat attcagcgac
     2101 gacctgtcaa tggccggtgc ccgggtgatc gatggacagc cggtcagcac cacgcaggcg
     2161 gctgtgctgg ccttgaacgc gggttgtgac atggtgttgc tgtgcaatca gtcgcttgga
     2221 gtcggcgagg agctcgatgc gctgatcaat ggcctggcgc aagcgcaact caaagggcag
     2281 tggctgccac gccaagcctg tgaagtgcgc agattggctt tgctgcccgc cagtgcggcc
     2341 atgccgtggg acgatttgat ggtgcagccg ggctacattc aggcgctcga tttgctgccc
     2401 tgaccactgc tgatggtggg tcgatggggt tgacaaccgg tgtggttgcc gcttagggtg
     2461 agcggtgcaa acgtggcgct gacaatgccg caatcatgtc caaggtggcc tggtccacat
     2521 tggggtgggc ggccagcagt cggccgaccg acagcagttt gcgcaaatcc tgcaggtctt
     2581 tcacgcttgg tgcgactttg atctgggcaa tcgctgccga gctgttgccg cccttgatca
     2641 atgccgcaac cttgggtgcc caaatgcctt gacgcgccat gtgctgcttt ctgtgaaatt
     2701 gaaaatacta aatgatgaaa ttggcctgta acgcttgcca tatatgcgct gatagctacc
     2761 ttaaatatag catgccgtga tccgctgagg caggccgccg acatgctcga caatcggcag
     2821 tccgaacaat ggcgataacc cgatgaaagc aaaacccaca catggcggct tggtttatag
     2881 caccgatagc ggtcgcacct gcccgctgtg tcgccaagct gtgtcgctgt gccaatgcag
     2941 cgccgccaaa gccaggccag cttcggatgg cattgtgcgg gtttcgcgtg aaaccaaggg
     3001 tcgcgctggc aagggggtta cgctggtcaa gggtttggct ttggacgatg ccgcgctcag
     3061 cgcgttgggc cgactgctca aaaccgcctg cgggtcgggt ggcacggtga aagacggcgt
     3121 gattgaggtg cagggtgacc attgcgaacg cgtgctggaa atgctcaaag ccaggggtct
     3181 ggtggtcaaa tgagta
//
LOCUS       DAOSLL010000570         3196 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_26358_length_3196_cov_3.954791, whole genome shotgun sequence.
ACCESSION   DAOSLL010000570 DAOSLL010000000
VERSION     DAOSLL010000570.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26358_length_3196_cov_3.954791"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(384..1316)
                     /locus_tag="PLB25_19105"
     CDS             complement(384..1316)
                     /locus_tag="PLB25_19105"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE43716.1"
                     /translation="MPSTLTQFDQPGTWKQAHRGLTNAVAPVSVPLPDFTEQRLWTRF
                     LEDWCEPGQRAPSIGFLGDGHEIVALHNGLALSSVFQPILSASTLLPMAFEALVRAKT
                     LKGGQVVSPDELFARAGSQAQGILLDRVCRFIHLLNFEHQAPDSARLYLNVGALHLQA
                     LQRGQHGSFMGAMQTLCSVVPTRVILEITETRFNDRDALSAIVQSFKQRGFRVAIDDF
                     GARHSNFDRLWALTPDIVKIDRELLLQADSNPRVRTILPKLVEIMHDLQASVVCEGIE
                     TSQQHDLALSSGADLLQGYFYAKPASHLKPSLIR"
     gene            complement(1329..>3196)
                     /locus_tag="PLB25_19110"
     CDS             complement(1329..>3196)
                     /locus_tag="PLB25_19110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013541113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment cargo
                     protein cysteine desulfurase"
                     /protein_id="HOE43717.1"
                     /translation="TPVLSLAHRTPALLPHAQAGNGLPDNLVTQLPAYEPRFGSGVLG
                     VPEAVGPSETPAPALPQRPQGAKPAGRQVPNASPFYFLNDGFGHPLAGYFSATAPIQD
                     GFSHIDLAQPGFDAPAMAAQPSYTQPITTPPAVSLPQGFAAPATPRAAPPYASAAPTR
                     PATAAPVAEPVPAGVITTAPKYYFVDAVVLPNGYVTPVKSAPEASTSIAHSDPHPAFD
                     VNAIRRDFPILQERVNGRQLVWFDNAATTQKPQSVIDRLAYFYAHENSNIHRAAHELA
                     ARATDAYESAREKVRAFINAPDVNEVIFVRGTTEAINLVAKSWGDQHVGEGDEIIVSH
                     LEHHANIVPWQQLAAAKGARLRVIPVDDSGQVLLDEYARLLNDRTKIVSVTQVSNALG
                     TVVPVKQIVEMAHRAGAIALVDGAQSVSHMPVDVQDISADFFVFSGHKVFGPTGIGAL
                     WGKREVLEDMPPWQGGGNMIADVTFEKTVYQPIPNKFEAGTGNIADAVGLGAAIDYVT
                     RIGMHNIDRYEHELLCYGMQQLGQIAGVRLVGTAADKASVMSFVLAGYSTEEVGNALN
                     EEGIAVRTGHHCAQPILRRMGLEATVRPSLAFYNTFEEVDRLVAVVRRLAQARAH"
BASE COUNT          646 a          941 c          956 g          653 t
ORIGIN      
        1 cgccgactgt ttgtgtagga gtcgcgccct cgcgacgaag gctcatcgat tccgggtcgt
       61 ccggcttggg ggtctgttgg actgatggat cgaacagcga gaagcaaccc cggcaaggcg
      121 tgcctgattg tccggtccgt agcccggttg tcggtcaaaa agccggttca aatgggccgc
      181 tgcgatcgat tcgcgatcaa tgctcgataa gcccgatgag ctcctggtag tccgttccat
      241 tcaaacgaat gcatgaaatc gtgtcttttt ccgcctggcg ttgttgctcg tcgttgtcat
      301 ggctgcggct agctcgcctc ctcgtgcata tccagacgga aaaatccatc aatttcattt
      361 tgcagccatt ttgatggaat ggactaccgg atgagtgatg gcttcaaatg gctggcaggc
      421 ttggcataaa agtagccttg tagcaaatct gcccctgaac tcagcgccaa gtcgtgttgc
      481 tggctggttt cgataccttc gcacaccacg cttgcctgca ggtcgtgcat gatctcgacc
      541 agcttgggca aaattgtgcg cacgcgcggg ttgctgtcgg cctgcaacag caattcacgg
      601 tcgatcttga cgatatcggg tgtcaaggcc cagagccggt cgaagtttga atgacgcgca
      661 ccaaagtcgt caattgccac ccggaaaccg cgttgcttga aactttgcac aattgcactc
      721 agagcgtccc ggtcattgaa gcgcgtttcg gtgatttcca gaatgacgcg agtcggcacc
      781 actgaacaaa gagtttgcat tgcccccatg aacgaaccat gctggcctcg ctgcagggct
      841 tgaagatgca gggcaccgac gttcaaatac agccgcgctg aatcaggcgc ctggtgttca
      901 aagttgagca ggtgaatgaa gcggcaaacc cggtccagca aaatgccttg agcttgtgag
      961 cctgccctgg caaacagttc atcgggcgac accacttgac cacccttgag ggtcttggcc
     1021 ctgaccaaag cctcaaaagc cattggcaac agggttgacg cactcaagat tggctgaaaa
     1081 accgaactca acgccaggcc gttatgcagc gcaacaattt catgcccatc acccagaaaa
     1141 ccgatgctcg gtgccctttg acccggttca caccagtctt ccaaaaaacg cgtccaaagc
     1201 cgttgttcgg tgaaatccgg caatggcaca ctgaccgggg cgaccgcgtt ggtcagaccc
     1261 ctgtgtgcct gcttccacgt gcccggttgg tcaaactgcg tcagcgtgga aggcatggca
     1321 aggaagcgtc aatgcgctcg tgcctgagcc agccgtcgta ccaccgccac cagccggtcc
     1381 acctcttcaa aggtgttgta aaacgccaat gagggccgca ctgtggcctc cagccccatg
     1441 cgccgcagaa tcggctgggc gcaatggtgg ccggtgcgca cggcaatgcc ttcttcgttg
     1501 agcgcattgc cgacctcttc ggtgctgtag cccgccagca caaaggacat cacgctggcc
     1561 ttgtccgctg ccgtgccaac cagccggaca ccagcaatct gcccgagttg ctgcatgccg
     1621 tagcacaaca gctcatgctc gtaacggtca atgttgtgca tgccgatgcg ggtaacgtaa
     1681 tcaatggcag cgcccagacc caccgcgtcg gcaatgttgc cggtgccggc ctcaaacttg
     1741 ttgggaatcg gctgatagac ggttttctcg aaggtcacgt cggcaatcat gttgccgccg
     1801 ccttgccagg gcggcatgtc ttcgagtacc tcacgcttgc cccacaaggc accaatgcca
     1861 gtcgggccaa acaccttgtg gccggagaag acaaagaagt cagcgctgat gtcctgcaca
     1921 tcgactggca tgtgcgacac cgactgggca ccatcgacca gtgcgatggc cccggcccgg
     1981 tgcgccatct cgacgatttg cttgaccggc accaccgttc cgagcgcatt ggagacttgc
     2041 gtcaccgaca cgattttggt gcggtcattg agcagtctgg cgtattcatc aagcagcacc
     2101 tggcctgagt catcgaccgg aatcacccgc aacctggccc ccttggcggc ggccagttgt
     2161 tgccagggca cgatgttggc gtggtgttcc aggtggctga cgatgatctc gtcaccctcg
     2221 cccacatgct gatcgcccca gcttttggcc accaggttga tggcttcggt ggtgccgcga
     2281 acaaagatga cttcgttgac atcgggtgcg ttgatgaagg cgcgcacctt ttcccgcgca
     2341 ctctcgtagg catcggtggc ccgggccgcc agttcatgcg cggcacggtg gatgttggag
     2401 ttttcatgcg catagaaata cgccagacgg tcaatcaccg actgcggctt ttgcgtggtg
     2461 gcggcgttgt caaaccagac cagttggcgg ccgttcactc gctcctgcag aatcgggaaa
     2521 tcgcgccgga tggcgttcac atcaaacgcc ggatgtgggt cactgtgtgc tattgaagta
     2581 gaagcttccg gcgcagattt gacgggggtt acatagccat ttggcaacac aactgcatcg
     2641 acaaaatagt attttggcgc ggtcgtgatg accccggcgg gtaccggttc agcaaccggc
     2701 gctgccgtcg caggccgcgt cggcgcagcg ctggcataag gcggggccgc ccggggcgtt
     2761 gctggtgcgg caaatccctg gggcaggctg actgcgggcg gcgttgtgat gggttgggtg
     2821 tacgacggct gggctgccat tgcgggtgca tcaaagccag gttgcgccaa gtcaatgtga
     2881 gaaaagccgt cctgaatcgg cgccgtggcg gaaaaatatc ccgccaaagg gtgaccaaaa
     2941 ccatcgttca aaaagtaaaa cggtgacgca ttcggcacct ggcgaccggc gggcttggcc
     3001 ccttgcggcc tctggggcaa ggctggcgcg ggggtttctg acggcccaac agcttcgggc
     3061 acgcccagca caccgctgcc aaagcgtggc tcgtaggccg gcaactgtgt caccaggttg
     3121 tcgggcagac cattgccggc ctgcgcatgc ggtagcagcg ccggagtccg gtgcgccagc
     3181 gacagcaccg gtgtgg
//
LOCUS       DAOSLL010000571         3193 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_26387_length_3193_cov_4.561185, whole genome shotgun sequence.
ACCESSION   DAOSLL010000571 DAOSLL010000000
VERSION     DAOSLL010000571.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3193
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26387_length_3193_cov_4.561185"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1935)
                     /gene="lptD"
                     /locus_tag="PLB25_19115"
     CDS             complement(<1..1935)
                     /gene="lptD"
                     /locus_tag="PLB25_19115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HOE43718.1"
                     /translation="MLDASIRALLGRWRSPGIWAVGASLLPLVGHAQSSIEEAQQLKD
                     SPMLQERIDTATRSSLPTFVTGDQISGRPDLETIIEGQAKLRRGDSVIKADRLEYHQP
                     TDLVKAQGHVHINRAGNVYEGPLLELRVDAFEGFFNQPGYQFLKNQAHGQADRVDFLD
                     DQRAVIHNATFTTCKRRPGPDWLPDWILKATTLSIDTEEEVGTAQGALLSFKGMPLLP
                     VPYLTFPLSDQRKSGFLPPTFGIDSVNGAELSQPYYWNIAPNRDATLTPTLMSNRGLN
                     LGAEFRYLEPDYSGATRLDWMPVDALRDSSRWGLNVKHQQHIASAWTNNGLALKLDLN
                     RVSDDNYWRDFTHTNGSLTQRLLASDGSLSWANGPFFSTIRTLKWQTLQDVSSPIVPP
                     YDRLPQLATRYARNDLGGYNLSVDADFTRFVSDSSLTGQPNGRRLFSMLQVNRSWLTP
                     AGFITPKLQLHATNYQFDAPLANGQSQATRVVPTFSLDSGLVFERDAELFGRSLRQTL
                     EPRAFYVNTPYRDQNLLPNYDSGVSDFNLASIYSENAFVGNDRISDSHLLTFGVTSRY
                     LNPSTGAEVVRLGVAQRWRFRDQNVTLPGDEPVKDRFSDLLLGGSIRWDPGWSVDATV
                     QLNPQSGDSVRSTLSARYNPG"
     gene            2038..>3193
                     /locus_tag="PLB25_19120"
     CDS             2038..>3193
                     /locus_tag="PLB25_19120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HOE43719.1"
                     /translation="MSQAHTPQTPEMTGIVWTDLQRATQFNVWLAQVAPDQLLNTGTL
                     RMASADASFRRYFRIDSGRGSRIIMDAPPQQENCRPFVEVARLMADAGLCAPVVLAWD
                     EPLGFMLMTDLGGQTMMQVIDQDHAQANLPLYLQAVDALIAWQRSSRPDVLPPYSEAL
                     LRRELELFPEWYLGRHKGLTVAGDMRITLDQAFNKIIATNLAQPSVYVHRDFMPRNLM
                     APKPVAACVASPPEVAAATWCSPATGLDAQQTPVSALGVLDFQDAVYGPITYDIASLM
                     RDAFLSWEEDFCLDVTVRYWEKARKVGLPVGDDFGEFYRNVEWMGLQRHLKVAGIFAR
                     LTLRDGKPQYLADSPRFIGYIRNTCARYGELKPLLRLVDKVEGIVEPAVFG"
BASE COUNT          682 a          933 c          922 g          656 t
ORIGIN      
        1 gcccgggttg tatcgtgcgc tcagggtgga ccgcaccgaa tcccctgact gcgggttcaa
       61 ctgcaccgtg gcgtcaacgc tccagcccgg gtcccaccga atggagccgc ccaacaacaa
      121 gtcactgaaa cggtccttca ccggttcatc acccggcaga gtaacgttct gatcacgaaa
      181 tcgccagcgt tgtgcaacgc ccagacgcac cacttcagcc ccagtagatg ggttcaaata
      241 ccggctggtt accccgaagg tcaacaaatg gctgtcggaa atgcggtcat taccgacaaa
      301 agcgttctca gagtaaatgc tggccaaatt gaaatcgctg acacccgagt cgtaatttgg
      361 caacaggttc tggtcccggt aaggcgtgtt gacataaaac gccctaggtt caagcgtctg
      421 gcgcaagctg cgcccgaaca attcggcatc ccgttcaaac accaatccac tgtcaaggct
      481 gaaagttggc accacccgtg tcgcctggct ctgcccattg gccagtggcg catcaaactg
      541 gtagttggtc gcatgaagct gcaatttagg cgtgatgaac ccggccggtg tcaaccagga
      601 tcgattgact tgcagcatgg aaaacagtcg ccgcccattg ggctgaccag tcaaactgct
      661 gtcagaaaca aagcgtgtga aatctgcatc caccgaaagg ttgtagccac ccaggtcatt
      721 gcgcgcgtaa cgggtcgcca gttgtggcag ccggtcatag ggcgggacaa tcggcgaact
      781 gacatcttga agcgtttgcc atttgagggt ccgaatcgtg ctgaaaaacg gcccgttggc
      841 ccacgacaag ctgccatcgc tggccaacag acgctgcgtc agcgagccat tggtgtgggt
      901 gaaatcacgc cagtagttgt catcgctgac gcggttcagg tccagcttca aggccaggcc
      961 attgttggtc caggcactgg caatgtgctg ctggtgtttg acgttcaaac cccagcggct
     1021 gctatcgcgc agcgcgtcaa ccggcatcca gtccagtcgt gttgcacctg aatagtctgg
     1081 ctcaaggtac ctgaactcgg ctcccagatt gagaccccga ttgctcatca gcgtgggagt
     1141 caaagtggca tcgcggttgg gggcgatgtt ccagtaatag ggctgcgata actccgcgcc
     1201 attgacactg tcgatgccga aggtcggcgg caagaacccg gatttgcgct gatcgctcag
     1261 tggaaaagtc aggtaaggaa ccggcagcaa cggcatccct ttgaaactca gtaaagcgcc
     1321 ttgtgcagtg ccaacctctt cttcggtgtc aatgctgagg gtggtggcct tgagtatcca
     1381 gtccggcagc caatccggtc cgggcctgcg cttgcaggtg gtgaatgtgg cgttgtgaat
     1441 cacggcacgc tggtcgtcca ggaagtccac ccggtcggcc tgcccgtgcg cctggttctt
     1501 cagaaattgg tagcccggct ggttgaaaaa tccttcaaac gcatccaccc ggagttccag
     1561 caatggacct tcgtacacgt tacccgcacg gttgatgtgc acatgaccct gcgccttgac
     1621 caaatcggtc ggctggtggt actccagacg gtctgctttt atgactgagt cgcccctgcg
     1681 caacttggcc tggccttcaa tgatcgtttc cagatcgggg cgaccgctga tctgatcgcc
     1741 agtgacaaac gttggcaagc tgctgcgtgt tgccgtgtca atgcgctcct gcagcatggg
     1801 gctgtccttg agctgctgcg cctcttcaat acttgactgt gcatgcccga ccagcggtag
     1861 cagcgatgcg ccgacggccc aaatacccgg cgagcgccag cgacccagga gagctctgat
     1921 ggaagcgtca agcatcaaga agcacatcgg acaattggcg tgacaaatag gtaggtagga
     1981 atggcgacaa catgaacgga ccgggcttgc acattgggtt tgtagaatgg attatccatg
     2041 agccaagccc acaccccaca aacacccgag atgaccggca ttgtctggac cgacttgcaa
     2101 cgtgcaacgc agttcaacgt ctggctggcg caagttgccc ctgatcaact cctcaatacc
     2161 ggcacactgc gcatggccag tgcagatgcc agcttcagac gctatttccg catcgattca
     2221 gggcgtggca gccgcatcat catggacgca ccaccccagc aggaaaactg tcggccattt
     2281 gttgaagtgg cccgtctgat ggccgacgca ggcctgtgcg cacccgtggt attggcctgg
     2341 gacgagccgc tagggttcat gctgatgacc gatctggggg gccagaccat gatgcaggtc
     2401 atcgaccagg accatgcaca ggccaacctg cctctgtatc tgcaggcagt tgatgcgctg
     2461 attgcctggc agcgcagttc ccgtcccgac gtgctgccac cttacagcga agcgctgcta
     2521 cgccgcgaac tggagctgtt tcccgagtgg tatctggggc ggcacaaagg actgaccgtt
     2581 gcaggtgaca tgcgcatcac actggaccaa gccttcaaca agatcatcgc caccaacctg
     2641 gcacaaccca gtgtttatgt gcaccgtgat ttcatgcccc gcaatctgat ggctccaaag
     2701 ccagtcgctg cctgcgtcgc gagtccccct gaagtggccg ccgcaacctg gtgtagcccg
     2761 gcaaccgggc tggacgcgca gcaaaccccg gttagcgctt tgggtgtgct ggactttcag
     2821 gatgcggtct atggcccgat cacctacgac attgccagtt tgatgcgcga tgcctttttg
     2881 agctgggagg aagatttttg cctggatgtc accgtgcgct actgggaaaa ggcccgcaaa
     2941 gtcggcctgc cagtcggcga cgactttggc gagttttatc gcaacgtcga gtggatggga
     3001 ctgcaacgcc acctgaaagt cgccggcatt tttgcccgat tgaccctgcg tgacggcaag
     3061 ccgcagtacc tggccgactc gccgcgtttc atcgggtata tccgcaacac ttgcgcacgc
     3121 tacggcgaac tcaaaccgct gctgcggttg gtggacaagg tggaaggcat tgtggagcca
     3181 gcagtattcg gat
//
LOCUS       DAOSLL010000572         3191 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_26412_length_3191_cov_3.850765, whole genome shotgun sequence.
ACCESSION   DAOSLL010000572 DAOSLL010000000
VERSION     DAOSLL010000572.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26412_length_3191_cov_3.850765"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PLB25_19125"
     CDS             <1..396
                     /locus_tag="PLB25_19125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013519309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HOE43720.1"
                     /translation="IAKPFSTKELLARIRAVLRRRAPEQDAGVVTVGALCLDAATYRV
                     TFREQPVKLGPTEFKLLHYLMTYPERVHSRSQLLDKVWGDYALIEERTVDVHVKRLRE
                     SLGSEAGAKIETVRGAGYRITALECTGAV"
     gene            496..1200
                     /locus_tag="PLB25_19130"
     CDS             496..1200
                     /locus_tag="PLB25_19130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010467012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HOE43721.1"
                     /translation="MNQHLLLIEDDVRLANMVSEYLRQSGYLVDHAGDGHGGLTMLQS
                     ITPNLVVLDLMLPDMDGLEICRRIRALPGALGATPILMLTAKGDPMDRIIGLEMGADD
                     YLPKPFEPRELLARIRAVLRRQGDGASVASTHLRFGTLEIDRDARIVSVAGQACELTS
                     YQFDLLVALAERAGRVLTRDQIMEAVRGRELDAFDRSIDVHMGRIRAAIEVDAKAPKR
                     ILTVRGVGYVFARQQD"
     gene            1267..2604
                     /locus_tag="PLB25_19135"
     CDS             1267..2604
                     /locus_tag="PLB25_19135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HOE43722.1"
                     /translation="MLNKLYLRIWLAVVLAVAVLTLLVGWIWRLAAEPPLREVVVRSD
                     TGQIIGRGRQHLPPGEADTRLDGDQSAGPPYRRRSKGPVLPPEELAIASASEAASANQ
                     PDPDRPVIPEFMVRMHDGQTMRMRFVRPPASFWSRPPFGFAWMLVWVGIAVALATYPI
                     VRTLTRRLERLQDGVQQWGDGDLSIRVSEAGQDEIAFLARRFNGAAERIQTLVKSHEV
                     LLASQKSLLANASHELRSPLARIRMGLELLGPSATPAFKTEIARNISELDQLIEEILL
                     ASRLDAREADMGTVESVDLIGLAAEECARVDAELDVLTQSTASGPSQVASELAVQGVA
                     KLLRRAVRNLLENAHRHAASDISLTLRHNGHQVEIRVCDRGPGVPEALQQRIFEPFYR
                     LPGATERDGGVGLGLALVKSIAVRHGGRVFCESRAGGGACFVIELPLANQKTT"
BASE COUNT          589 a          900 c         1026 g          676 t
ORIGIN      
        1 attgccaagc cgttttctac caaagaactg ctggcccgga ttcgcgccgt gctgcgcagg
       61 cgtgcgccag agcaggatgc aggcgtggta acggtcgggg cgctgtgtct agatgctgcc
      121 acctaccgcg tgacgttcag ggaacaaccg gtcaagctgg gtccaaccga gttcaagctg
      181 ctgcactatt tgatgaccta tccggagcgt gttcacagcc gatcccagtt gcttgacaag
      241 gtctggggag actatgccct gattgaggag cggacagtgg atgtccacgt caagcgactg
      301 cgggaatcgc tgggatctga agcgggtgca aagattgaga ccgttcgcgg cgcaggatac
      361 cgaatcacgg cgcttgagtg cacgggtgct gtttgaactg actattcagc gtttttgggg
      421 ctgatgggct gttacttcac cccaacttta cctgcccttt aagtcggcat tgtggcgcac
      481 ggctagactc aagtcatgaa ccaacacctg ctgctgatcg aggacgatgt gcgtctggcc
      541 aacatggtaa gcgaatacct gcgccagtct ggctacctgg tggatcacgc tggcgacggc
      601 cacggcggcc tgaccatgct gcagagcatc acccctaatc tggtggtgct tgacctgatg
      661 ctgcccgaca tggatggcct ggagatttgc cgtcgcattc gcgccctgcc cggtgcgctg
      721 ggtgccacgc cgatcctgat gctgacggcc aagggcgacc cgatggaccg cattattggc
      781 cttgagatgg gtgccgacga ctatttgccc aagccctttg agccgcgcga attgctggcg
      841 cgcatccgtg cagtgctgcg ccgccagggc gatggggcgt cggtggccag cactcatttg
      901 cgttttggca cgctggaaat tgaccgtgat gcccgcattg tgtcggtcgc cgggcaggcc
      961 tgcgagctga cctcttacca gtttgatttg ctggtggcgc tggccgagcg ggctggccgg
     1021 gtattgacac gtgaccagat catggaggcg gtacgcggtc gtgagcttga cgcttttgac
     1081 cgctcgattg atgtgcatat gggccgcatc cgcgcggcca tcgaggttga tgcaaaagcc
     1141 cccaagcgca tcctcaccgt gcgcggtgtc ggttatgtgt ttgcccgtca gcaagactga
     1201 ctgcgacacc acaggctccg ggcggcaccg caaatttgcc ttgttgctga cctgacaggc
     1261 tagcccatgc tcaacaaact ttacctccgc atctggctgg ccgtggtgct ggcggtagcg
     1321 gttttgaccc tgctggtcgg ctggatctgg cggctggcgg ccgagccacc gctgcgagag
     1381 gtggtggtgc gtagcgatac cgggcaaatc attgggcgcg gtcggcaaca cttgccaccc
     1441 ggcgaagccg acacccggct cgatggcgac cagtcggcag gtccacccta tcggcggcgt
     1501 tccaagggtc cggtcttgcc gcctgaagaa ctggcaattg cgtcggcaag cgaggcggca
     1561 tcggcgaatc agcctgatcc ggatcggccg gtcattcccg aattcatggt gcgcatgcac
     1621 gacggccaga ccatgcggat gcgctttgtg cgcccgccag cctccttttg gagccgcccg
     1681 ccgtttggtt ttgcctggat gctggtgtgg gttggcattg cggtggcgct ggccacctac
     1741 ccgatagtgc gcaccctgac gcgccgactg gagcgcttgc aagacggcgt gcagcaatgg
     1801 ggtgatgggg acttgtcgat ccgcgtgtct gaggccggac aagacgagat cgcctttctg
     1861 gcgcgccgtt tcaacggcgc ggctgaacgt attcagaccc tggtgaagtc gcacgaagtg
     1921 ctgctggcct cgcaaaagtc tttgctggcc aacgcctcgc atgagctgcg ttcgccgctg
     1981 gcgcgcattc gcatggggct ggaactgctc gggccgtcgg ccacgcccgc cttcaaaacc
     2041 gagattgccc gcaacatcag cgagctggac cagttgatag aggagatttt gctggccagc
     2101 cgacttgacg cgcgtgaggc cgacatgggc acagtcgagt cggttgacct gatcgggctg
     2161 gccgctgaag agtgcgccag ggttgatgcc gagctcgatg tgttgaccca aagcaccgcg
     2221 tctgggccct cgcaggtggc cagcgaactg gcggtgcaag gcgtggccaa actgctgcgc
     2281 cgtgccgtgc gcaacttgct ggagaacgcg caccgccacg ccgcgagcga tatcagcctg
     2341 acgctgcgcc ataacggtca tcaggttgaa atccgggtgt gtgaccgtgg cccaggcgtt
     2401 ccagaggcgc tgcagcagcg catttttgag ccgttttacc gtttgccagg tgctaccgag
     2461 cgtgatggcg gtgtcgggtt ggggttggcg ctggtgaaat cgatagcggt gcgccatggt
     2521 ggccgggttt tttgcgaaag ccgggcgggt ggcggagcct gttttgtcat tgaattgcca
     2581 cttgccaacc aaaaaaccac ctaaaaccgc aaaataagtt gatttatctt caaaaacccc
     2641 ttcaatatcc cccaaatggg ggatacccaa acgctgtctt gcccggtaaa gttcatctag
     2701 ctgctgtcac agcgtcgaag ctaacaattt tggatcgtgt caagcgcaag tttgaccgtc
     2761 ggagagttcc aacgacgttc ccccatgggg aaccttcaca agccacctca aggtggcttt
     2821 ttttttgttc gcccagcatg ggcgcactcc tgcaggtgct ggtcccgtcg cgagctgacc
     2881 accgtgagcg aagtgaatcg caactgcagg cgggtgactg actgtgggaa gcaagcgcgg
     2941 cacatgaatc atgagtcaat gtatgaaagt cagatatgac gcggtgtcga tcagggtgag
     3001 cgggtgcaag atcgcgaagg ttttgtcgcc aaagatcgat ggcgtttggc cgttgggcta
     3061 gaaggcccat tttggtttgt cgcaaacaac aggaatctgg cgcacgctgc atgcagggtt
     3121 gcggcatcga ctcggggcga ttgggcttga acattgcaag cctgccccaa cgatctttgt
     3181 tgagtcactt g
//
LOCUS       DAOSLL010000573         3176 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_26550_length_3176_cov_3.792054, whole genome shotgun sequence.
ACCESSION   DAOSLL010000573 DAOSLL010000000
VERSION     DAOSLL010000573.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3176
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26550_length_3176_cov_3.792054"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2034
                     /gene="mrdA"
                     /locus_tag="PLB25_19140"
     CDS             <1..2034
                     /gene="mrdA"
                     /locus_tag="PLB25_19140"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011481199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HOE43723.1"
                     /translation="AELTRFRVRVLVASLVVVACFGLVVSRLIYLQVVRHDDLRAQAE
                     NNRTAIVPIVPNRGLILDRNGIVLASNYSAYTLEVTPSKVANLEQTIDELSTVLEIAP
                     RDRRRFKKLREEAKSFESLPLRSRLSDAEVAKFAAQRYRFPGVDIRARLFRNYPFGEL
                     ASHVIGYIGRINAAEKEMLEESDDPANYRGTEYIGKLGVEQSFEPQLHGITGVDSMET
                     SAGGRATRRLASQSSTPGNTVRLSIDIKLQKLIEDMYGDRRGALVALDPKSGEVLAFV
                     SKPTFDPNLFVEGIDQESWQSLNESLDKPLLNRALRGTYPPGSTYKPFMALAALQTNT
                     RSASVQINDPGFFMFGGHRFGSPENERGGIMDMRRAIVESSNAYFYSLANELGVDAMH
                     DFMKPLGFGQITGIDIHGEVRGVLPSQAWKRSYFKKPEHQRWYAGETISLGIGQGYNS
                     FTMLQLAQATAILANNGVKHKPQLVIATQDTSTRAIVPLQPAPAENLGYKQEHVDVIR
                     RAMVGVTQEGTSTRVFAGAAYLSGGKTGTAQAVSLGKNEKYNASRMEERQRDHSLYIA
                     FAPADDPKIALALIVENAGFGSASAAPIARRVFDYWLLGHYPNEDDLLAVSKGQAAAP
                     LGKPRLVAEVPWPLTGVSAANAASSPAQGGAAIKPASAPVAPASTAASSLRRR"
     gene            complement(2021..3043)
                     /locus_tag="PLB25_19145"
     CDS             complement(2021..3043)
                     /locus_tag="PLB25_19145"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HOE43724.1"
                     /translation="MRYSQSKEQSAELLRLVLARMGQHDASFTPMTFTVWFEYAAGIN
                     LQLNDAIDRLMQSHPRLTDDDLWSLYQKYVADIDPQAMHRIGGELQRVMKTLANAASR
                     TGEHAGEFSTQLETLVAELRGVNDMLTTPAITHAIEGTARMRSSAQALESEVRNNQLE
                     IERLQGELTRVRDESLRDALTLVLNRKGFDLKLAEMLGRVPAPGSAHGLIMFDLDFFK
                     KVNDTHGHVMGDRVLQALGEVLRSCVPASSSSSAARYGGEEFAVLMPDSTATECQKLA
                     ELVRQRTKALKIRDRRTQTVVLTVTISGGITLMRPDDDAQTFTARADGALYQSKQGGR
                     DRVTFA"
BASE COUNT          623 a          949 c          970 g          634 t
ORIGIN      
        1 gccgagttga cgcgcttccg ggtgcgggtt ctggtggcga gcctggtggt cgtggcgtgt
       61 tttgggctgg tggtgtcgcg tctgatttac ctgcaggtgg tgcgacacga cgacctgcgg
      121 gcacaagccg aaaacaaccg gaccgccatc gtgccgatcg tgcccaatcg cggtctgatc
      181 ctggaccgca acgggatcgt gctggcgagc aactattcgg cctacacact ggaggtgacg
      241 ccttctaaag ttgccaatct ggagcagacc attgacgaac tctccactgt gcttgagatc
      301 gctccacgtg accgaaggcg cttcaagaaa ttgcgcgaag aagccaaaag ttttgaatcg
      361 ctgccgctgc gtagtcgcct gagcgatgcg gaggtggcca aatttgccgc ccagcgctac
      421 cgttttcctg gcgttgacat cagggcgcgg ctgtttcgca actacccgtt tggtgaactg
      481 gccagtcacg tgattggcta catcggtcga atcaacgccg ccgaaaagga aatgcttgag
      541 gagtctgacg acccggccaa ctaccgtggt accgagtaca tcggcaaact gggtgtcgaa
      601 cagagtttcg agccccagct gcatggcatc acgggcgtcg actccatgga aacttcggcg
      661 ggtgggcgcg cgacccggcg cctggccagt cagtcgtcca cacccggcaa caccgtcagg
      721 ctgtccattg acatcaagtt gcagaaactg atagaggaca tgtatggcga tcgccgcggg
      781 gcactggtcg cgctggaccc caagtcgggc gaagtcctgg cctttgtcag caaacccacg
      841 tttgatccca acctgtttgt cgaaggcatt gaccaggaaa gctggcagtc tctcaatgaa
      901 tcactcgaca agcccctgct caatcgcgcc ctgcgtggta cttatccgcc gggctccacc
      961 tacaaaccgt tcatggccct ggctgcattg cagaccaaca cgcgctcggc ctccgtccag
     1021 atcaatgatc caggcttctt catgtttggg ggacaccgct ttggcagccc cgagaatgag
     1081 cgtggcggca tcatggacat gcgccgggcc attgtggagt caagcaacgc ctatttctat
     1141 tcgctggcca acgagttggg tgtggatgcc atgcacgact tcatgaagcc tttggggttt
     1201 ggccagatca ccgggatcga tatccatggc gaggtacgcg gtgttttacc cagccaggcc
     1261 tggaaacgca gttactttaa aaaaccggag catcagcgat ggtatgccgg ggagacgatc
     1321 tcgctgggca tcggccaggg ctacaacagc ttcaccatgt tgcagctggc acaggccacc
     1381 gccatactgg ccaacaacgg cgtcaagcat aagccccagt tggtgatcgc cacccaggac
     1441 accagcaccc gggcaattgt tccgctgcag ccggccccgg cagaaaacct ggggtacaag
     1501 caggaacatg tggatgtgat ccgccgggcc atggtgggcg tgactcagga gggcacgtcc
     1561 acccgggtgt tcgccggggc ggcctacctg agtggtggca agaccggcac cgcccaagcc
     1621 gtgtcgctgg gtaaaaacga aaagtacaac gcctcccgaa tggaagagcg ccagcgtgac
     1681 cactcgctgt acattgcctt tgcaccggca gatgacccca agattgcact ggcgctgatt
     1741 gtcgagaatg caggttttgg ttcggcatcg gccgcgccga ttgccagaag ggtattcgac
     1801 tactggctgc tgggtcacta ccccaatgaa gacgatttgc ttgccgtcag caaggggcag
     1861 gccgctgcgc ccctgggcaa gccacgcctg gtggcagagg tgccctggcc attgactggg
     1921 gtatcggcgg ccaacgcggc ctcgagtccg gcgcaggggg gcgcggctat caaacctgca
     1981 tcagcgcccg tcgcgcccgc cagtactgcg gcgagcagtc tcaggcgaag gtaacgcggt
     2041 cgcggccgcc ttgtttggac tggtacaaag cgccgtcagc gcgggcggtg aaggtctggg
     2101 catcgtcatc cgggcgcatc agcgtgatgc cgcccgagat cgtgaccgtc agcaccacag
     2161 tctgggtccg gcggtctcga attttcaggg ctttggtgcg ctgtctcacc aactctgcca
     2221 gcttctggca ttcggtcgcc gtgctgtctg gcatcagcac cgcaaactcc tcgccgccgt
     2281 agcgtgcggc gctgctactg gagctggcag gcacgcaact gcgcagtacc tcgcccagtg
     2341 cctgcaggac acggtcgccc atcacgtggc catgggtgtc attcactttc ttgaaaaaat
     2401 ccagatcaaa catgatcagc ccgtgcgcgc tgccgggcgc ggggacgcgg cccagcatct
     2461 cggccagctt caggtcaaag cccttgcgat tgagtacaag cgtcaaggca tcgcgcaggg
     2521 attcatcgcg cacgcgggtc agctcgccct gaaggcgttc aatttccagt tggttgtttc
     2581 tgacttccga ctcaagcgct tgcgctgaac tgcgcatgcg cgcggtgcct tcaatcgcgt
     2641 gcgtgatggc cggggtggtc aacatgtcgt tcacaccccg cagttccgcc accagggttt
     2701 caagctgcgt gctgaactcg ccggcatgct cgcccgtgcg ggaggctgca ttggcgaggg
     2761 ttttcatgac acgttgcagt tcgccgccaa tgcggtgcat ggcttgcggg tcaatatccg
     2821 ccacatactt ttggtacaag ctccagagat catcgtcggt caggcggggg tgcgactgca
     2881 tgaggcggtc gatggcatcg ttgagctgca ggttgatgcc cgccgcgtat tcaaaccaga
     2941 cggtgaaagt catcggggtg aacgatgcat catgctgacc catgcgcgcc agaacaagtc
     3001 gtagcagttc agcactttgt tcttttgact gtgaataccg catgttcagc tttcctggag
     3061 tgatgtgtcc aacgaactca ggattataac ttcccaagcc gcgcaaaccc gaccgtcttg
     3121 cgtgatattg gcggcgcctg cccgtctgcg gaaacgctgc caaaactccg ccagga
//
LOCUS       DAOSLL010000574         3172 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_26606_length_3172_cov_4.521335, whole genome shotgun sequence.
ACCESSION   DAOSLL010000574 DAOSLL010000000
VERSION     DAOSLL010000574.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26606_length_3172_cov_4.521335"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="PLB25_19150"
     CDS             <1..278
                     /locus_tag="PLB25_19150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43725.1"
                     /translation="TLYVLDEPTVGLHMADVDKLIRVLHRLVDGGHSVVVIEHDLDVI
                     AEADWIIDLGPEGGKAGGRIVAAAAPEDVVRAGTHTGVALGPVLARR"
     gene            complement(363..1469)
                     /locus_tag="PLB25_19155"
     CDS             complement(363..1469)
                     /locus_tag="PLB25_19155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012250880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HOE43726.1"
                     /translation="MNEPLPASQYPGLLADIKQRIGQAQTRAILAVNTELIRLYWEIG
                     QMLDARQKTEGWGAAVIPRLALDIRNELPEVKGFSERNIKRMLAFYREYPALEFMPPA
                     VAQIQAGGKVPQAVALFPAELVASIPWGHHLLLMEKTKNAATRQWYMKATLANGWSRS
                     ILQMQIETAAHTRQGKTTSNFALRLPPPQSDLVQQTLKDPYLFDFLTLTEPFHERELE
                     TGLIRHLEKFLLELGQGFAFVGRQHHLEVGEQDFYIDLLFYHLRLRCFVVIELKRGAF
                     KPEYAGKMNFYCSVVDDVLRHAQDAPTIGLILCQQQNAVIAEYALRGVDKPIGVSTFE
                     LTRALPAELASKLPSIEQIERELGGGEPETPESQ"
     gene            complement(1482..1628)
                     /locus_tag="PLB25_19160"
     CDS             complement(1482..1628)
                     /locus_tag="PLB25_19160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43727.1"
                     /translation="MDGMFFERQALESITPIKAQPWMAFGKLFSRKNSLFPSSNGHMQ
                     LFIL"
     gene            complement(1751..3016)
                     /locus_tag="PLB25_19165"
     CDS             complement(1751..3016)
                     /locus_tag="PLB25_19165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43728.1"
                     /translation="MSSFYQYRHSQVIDLWPVFEHEILPFAQGTSTMDIATLWHRLKT
                     SDPFIQSKASGEAAWYRKARYERYEADIARFNQVLGEVLAKITTEAQKFYGQHFAEPD
                     HQFELVVGLTATCSYDRHEHRISMPRIGFEVKLNGDAIRKPQSFLNEANLTQLALSVR
                     FGATLAHLHESPLKLLVLDDLLISLDMNNRMKVVDIILSETFAEHQKIILTHDLGFFN
                     EFRRRIGGRHAEWSFQRLVGSADSEIRLQDDKSQLQKAEEYLHGESLDEAANCIRKAA
                     EDTARRFRELTVVPTKDFLTLTEQLRWARNKLLEKLPTKLYEKVLKGVPEKHRQYLIP
                     ASDIDLSAIPAITSDELTDLSRLRKQLRTLVTSEHWVMIENAKVIDEVLATTERVLNP
                     GSHGGEAPLYKEEVRQALELVKQLERCLS"
BASE COUNT          649 a          874 c          917 g          732 t
ORIGIN      
        1 acacgctcta cgtgctggac gaacccaccg tgggcctgca catggccgat gtggacaagc
       61 tgatccgcgt gctgcaccgc ctggtcgatg gcggccacag cgtggtggtg atcgaacacg
      121 acctcgacgt gatcgccgag gccgactgga tcatcgacct cggccccgaa ggcggcaagg
      181 cgggtgggcg catcgtggcg gccgccgcgc cggaagacgt ggtgcgggcg ggcacgcaca
      241 ccggggtggc gctggggccg gtgctggcgc ggaggtgaag gagagtcgtt ttcagccgca
      301 gaagaaatcg gttcaaaata gatagctgac tatgaccatt ccacgctggg atagtgctat
      361 tttcattggc tttctggcgt ttcaggctcg ccaccgccca gctcgcgctc aatctgctcg
      421 atgctgggca gcttggacgc cagctcggcc ggcagcgcgc gggtcagctc gaaggtggag
      481 acgccaatcg gcttgtccac gccgcgcaag gcatattcag cgatcaccgc gttctgctgc
      541 tggcagagga tcagcccgat cgttggtgcg tcttgcgcgt gccggagcac atcgtccacc
      601 acgctgcagt agaaattcat cttgccggca tattccggct tgaacgcacc gcgcttgagc
      661 tcgatgacga caaagcaccg caggcgcagg tgataaaaca gcaggtcgat atagaagtcc
      721 tgctcgccaa cctccaaatg gtgctgccgc ccgacgaagg cgaagccttg ccccagttcc
      781 agcaggaatt tttccagatg ccggatcaac ccggtttcca gctcgcgctc gtgaaacggc
      841 tcggtcagcg tcaggaagtc gaacaggtag gggtctttca aggtctgttg cacaagatca
      901 gactgcggcg gtggcagtcg cagcgcgaag ttgctcgtgg tcttgccctg gcgggtatgg
      961 gcggcggtct cgatctgcat ttgcaggatg ctgcggctcc agccgttcgc caaggtggcc
     1021 ttcatgtacc actggcgggt tgctgcattc ttggtctttt ccatcagcag caagtgatgc
     1081 ccccaaggga tggacgccac caattcggcg gggaaaagtg ccacggcttg tggcactttt
     1141 ccaccggctt gaatttgtgc cacagccggt ggcataaatt caagcgcggg gtactcgcgg
     1201 taaaaagcca gcatccgctt gatgttgcgc tccgaaaagc ccttgacctc aggcaactcg
     1261 ttgcggatat ccagcgccag tctggggatg acggccgcgc cccagccctc agtcttctgc
     1321 cttgcatcca gcatctggcc aatttcccaa tacaggcgaa tgagctcggt attgaccgcc
     1381 agaatcgccc gcgtctgggc ctgcccgatg cgttgcttga tgtcggccag cagtccgggg
     1441 tactgcgatg caggaagtgg ttcgttcatg gctgggggct actacaaaat aaatagctgc
     1501 atatgaccgt tcgacgatgg gaagaggcta ttttttcttg aaaatagctt gccgaaagcc
     1561 atccagggct gggctttgat cggggtgatc gattccagtg cctgccgctc gaaaaacatg
     1621 ccgtccaagg cgttctgcgc ttgctgtcgc tacgtgcgcg accgcccggc cttgatacgc
     1681 ggcgaatcgg cgcaggcgtt gagttcggtg aagtggtctt gggtggtgtg cacataactc
     1741 gcggagttgg ttaagacaaa cacctttcga gctgtttgac cagttcaagc gcttggcgaa
     1801 cttcttcttt gtacagcggg gcttcgccac cgtgggagcc cggattgagg acgcgttcag
     1861 tcgtcgcaag cacttcgtct ataactttgg cgttttcaat catcacccaa tgttcactgg
     1921 taaccagcgt gcgcaactgc tttctcaatc gactgagatc ggtcagttca tccgaggtga
     1981 tcgcaggtat tgccgaaagg tcaatatccg aagcagggat aaggtactgc cggtgctttt
     2041 ccggcacgcc cttcaacact ttctcgtaga gctttgtagg aagcttctcc aacagcttgt
     2101 ttcgtgccca acgtaactgt tcggtcaaag tcaaaaaatc cttggtgggt acaactgtga
     2161 gctcacgaaa acgtcgggca gtatcttcgg ctgcctttcg aatgcaattg gccgcttcgt
     2221 ccagactctc gccgtgcaga tattcctctg ctttttgaag ttggcttttg tcatcttgca
     2281 accgaatttc agagtcggcg cttccgacta agcgctgaaa tgaccactct gcatgtcggc
     2341 caccaatccg ccgcctgaac tcgttgaaga atcccaaatc gtgcgtcagg atgattttct
     2401 ggtgctcggc aaacgtttcg ctcaagatga tgtccaccac cttcatgcga ttgttcatgt
     2461 ccaggctgat caataaatcg tccagcacca gcagcttcaa gggtgattca tgcagatggg
     2521 ccagcgttgc gccaaagcgg actgacaggg ccagttgggt cagattggcc tcattcaaaa
     2581 atgactgcgg ctttctaatg gcatcgccgt tgagtttgac ttcaaaacca atgcggggca
     2641 tggaaatgcg atgttcatga cggtcataac tgcaagttgc agttaagccg acaaccaact
     2701 caaattggtg gtctggctct gcaaaatgct ggccataaaa cttttgagcc tcagtcgtta
     2761 ttttcgcaag aacctctccc agcacctgat tgaaccgggc aatgtccgct tcatagcgct
     2821 catagcgtgc ctttcgatac caggcggctt ctccgctggc cttcgactgg ataaatggat
     2881 cggaggtctt cagtctgtgc cacagcgtgg caatgtccat ggtggaggtg ccttgcgcaa
     2941 acggcaaaat ttcatgctca aaaaccggcc ataggtcaat aacttgtgag tggcgatact
     3001 ggtaaaaact ggacaacacc cggtaggtaa tgaagtcact ggccaggttg gctttcagta
     3061 catcggggtt cgccttggtg tcgtgctgtc cctttgacag cgagtagacg ctggccgttg
     3121 tgtagctgtc gtctttgatt gacacgacca cggctgcggc tgcgcgctct tc
//
LOCUS       DAOSLL010000575         3169 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_26640_length_3169_cov_4.463391, whole genome shotgun sequence.
ACCESSION   DAOSLL010000575 DAOSLL010000000
VERSION     DAOSLL010000575.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26640_length_3169_cov_4.463391"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..263
                     /locus_tag="PLB25_19170"
     CDS             51..263
                     /locus_tag="PLB25_19170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43729.1"
                     /translation="MADHPNRQAITQAVPAASHVAGRLPLALAEAAKVQNALTAAQRV
                     FDASPNGIIHRNRHAAWARATAGRGE"
     gene            complement(629..949)
                     /gene="dctP"
                     /locus_tag="PLB25_19175"
     CDS             complement(629..949)
                     /gene="dctP"
                     /locus_tag="PLB25_19175"
                     /inference="COORDINATES: protein motif:HMM:NF015445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HOE43730.1"
                     /translation="MLDGEPGRLLLSKHAAIDLVGLTFWDNGFKPFTANTPIYSPRDF
                     AKLRIRTMKSPTLADQFASLGAQSIQIDFHATGQALADGAVDAQENPWLQLSRCGITE
                     CKST"
     gene            complement(1147..2265)
                     /locus_tag="PLB25_19180"
     CDS             complement(1147..2265)
                     /locus_tag="PLB25_19180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019573685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator"
                     /protein_id="HOE43731.1"
                     /translation="MQGKQNSQLPTLLIVDDQPENLTVLGELLESDYVVRVASSGLRA
                     LKAAASSPQPDLILLDVMMPGIDGHEVLRHLRADPATQDIPVIFVTAMDEAHDEELGF
                     ELGAVDYIAKPIKPAVVLARVRTQVQLKQALTSLRNLNANLETEVRKRMRDNRIVEDV
                     AMRALASLAETRDSETGNHIRRTQAYVSLLAHALAQHARYTTALSIDTIDMLVKAAPL
                     HDIGKVGIPDYILNKPGPLDANEWQIMKRHTVIGASSIEHAMAGEADHTPLAFLHVAM
                     EIARSHHERWDGSGYPDGLQGEAIPLSARLMALADVFDALISWRVYKAAFSYEVARCI
                     ILEGRGQHFDPVLVDVFDARFDDFCAVAKRYADEQTST"
     gene            2852..>3169
                     /locus_tag="PLB25_19185"
     CDS             2852..>3169
                     /locus_tag="PLB25_19185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogenase component 1"
                     /protein_id="HOE43732.1"
                     /translation="MSVALKARIAEVFEEPGCDKNQGKGEKERKKGCTKQLAPGAAAG
                     GCAFDGAMIALQPIADVAHLVHGPIACAGNSWDNRHSASSGPQIYRSGFTTDTNELDV
                     IYGG"
BASE COUNT          645 a          906 c          937 g          681 t
ORIGIN      
        1 acggctaagc ttgcctgacc ggtgcgcgtg aggcaaaccg cctggtgtcc atggccgatc
       61 acccgaaccg ccaggccatc acccaggcgg ttcctgctgc cagccatgtc gccggtcgcc
      121 tgcctttggc gctggccgag gctgccaaag tgcaaaacgc actgaccgct gcgcaacgtg
      181 tttttgatgc cagcccgaac ggcatcatcc accgcaaccg ccatgcggca tgggccaggg
      241 ccaccgctgg aaggggcgag taacttagcc accttgcccg cccgccaccg gctgcgggtg
      301 tctcatcgaa ggttcccaga tctacaagcg tttttggcct ccagccctta tacagtatgc
      361 gctgcaagct atgctattgg tagtaatgtg gtgactgcca gccatttggg ttgacacgct
      421 ttacccaccg caatgggccg aactgccggt ttgccagtct tttggatgtg ctcagaaata
      481 acttccccta tttgtaccgg cggatttggg cgcacagcgt tgttgcaaac cgtttgtgta
      541 gccagttgca ccgcgcggct tgggcctagc cttgcacttg tcatcacgac ctcctctatc
      601 caagttgttc tcgcttgact ccatagcgtc aggtgctttt gcactcagtg ataccgcatc
      661 gcgacaattg cagccagggg ttttcctgcg catcgaccgc gccatcagcc agtgcctggc
      721 cggtcgcatg aaagtcgatc tgaatcgact gggcaccgag cgaggcaaac tgatcggcaa
      781 gcgtcgggct tttcatggtg cgaatgcgca gtttggcaaa gtcgcgcggg ctgtagatcg
      841 gggtgttggc ggtgaatggc ttgaatccgt tgtcccagaa ggtcagcccg accagatcaa
      901 tggcggcgtg cttggacaac agcagacgac cgggttcgcc atcgagcatg gcaaaagcac
      961 ttcgcgccgg ccaaaaaata accaggccgg tcagcaatat tgcatggctg gaatcgctga
     1021 actcaggctg gccgtgggta tcaggatcag atcaagtttg ccgactctgg ccgcgtccaa
     1081 cctctgggta gcactgccga gcttttggtt cgagtgcacg gtgaggggcc aagcggtcag
     1141 tttcgctcag gtgctggtct gctcgtccgc gtaacgcttg gcaacggcgc agaagtcgtc
     1201 aaaccgtgcg tcaaaaacat ccaccagaac cgggtcaaaa tgctgacccc gaccttcgag
     1261 aatgatgcat ctggctactt catacgaaaa agctgccttg taaacacgcc aggaaatcag
     1321 cgcgtcaaaa acatcggcaa gtgccatcag tcgcgccgaa agcggtatgg cttcgccctg
     1381 aagcccatcc gggtaaccac tgccatccca gcgttcatgg tgggagcgtg cgatttccat
     1441 cgcaacatga agaaaggcca gcggtgtatg gtccgcttca ccggccatcg cgtgctcgat
     1501 cgagctggca ccaatcacgg tgtgccgctt catgatctgc cattcattgg catcgagcgg
     1561 acctggcttg ttcagaatgt aatcaggaat gccaaccttg ccgatgtcat gcaacggtgc
     1621 cgccttgacc agcatgtcaa tggtgtcgat gctcagcgct gttgtgtagc gggcatgttg
     1681 ggccagcgcg tgcgccaaca aactgacata agcctgggtg cgccggatat ggttgccggt
     1741 ttcgctgtcg cgtgtttcgg ccagactggc aagcgcccgc atggcgacat cctcgacgat
     1801 ccggttgtcc cgcatgcgct tgcggacctc ggtttccagg ttggcgttca ggttgcgcaa
     1861 gctggtcagg gcttgtttga gctgaacctg cgttcggacc cgggccagca cgacggctgg
     1921 tttgatgggc ttggcgatgt agtccaccgc accaagctcg aagccaagct cttcgtcatg
     1981 cgcttcgtcc atggcggtga caaaaatgac cgggatgtcc tgtgtggccg gatcagcccg
     2041 cagatgtcgc aatacctcat ggccatcaat gccaggcatc atgacatcga gcaaaatcag
     2101 atcgggttgc ggcgaagatg ctgccgcctt cagcgcgcga aggccagagc tggcgacgcg
     2161 aacaacatag tccgactcca gcaactcgcc aagaacggtc aggttttccg gctggtcatc
     2221 gacgatcagc aaggtaggga gttgcgaatt ttgtttacct tgcacgaagc aaccgcctta
     2281 aaagggtcaa gttccgtgag caaatggctc aagtcggtat cctaatctac ttcgcgggcc
     2341 ttattgtcat gccgccaaaa gtaagcttga tgctggcgct gcacgggtgc ggcagcaacg
     2401 gctggcagca cggcttgtgg ctattttggc ggccaacctg gctggcttct gagtcatcag
     2461 ccttttttgg gttgtagccc tttctgcata agcgctacca gctataaata taatagtgtt
     2521 tcagggctgc gccagcgcgc ttggccgacc acacccgcgc tactggcggg cctgcggcgg
     2581 cctaacgatg gccccagtga gctagtcggc cagccaggtg ggattgcaga gtggttttga
     2641 cgatgctggg tgtcggttgg cggtgttggc ggatggcgca ccgatgagga tgtcaggcgg
     2701 cgcagtcaaa ccgtttgtcg caatcccttc aaaccggcca aattttcccg cctggggcgg
     2761 cgataaccag tcaaaacgcg gtattgaaag ctgtttgctg ctggcataaa aaatgcggta
     2821 ttgcctgcat tcatcccatc aggagtcttt catgtcagtc gcgctcaagg ccagaattgc
     2881 cgaagtgttt gaagagcccg gttgcgacaa gaatcagggc aaaggcgaaa aggagcgcaa
     2941 gaagggctgc accaagcagc tcgcgccagg tgccgccgca ggtggttgcg cctttgacgg
     3001 tgccatgatt gcgctgcaac cgattgccga tgtggcgcac ctggtgcatg gtccgatcgc
     3061 ctgcgccggc aactcctggg acaaccgtca tagcgcttcg tctggcccgc aaatttaccg
     3121 cagtggcttc accaccgaca ccaatgaact cgatgtgatt tatggcggg
//
LOCUS       DAOSLL010000576         3168 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_26649_length_3168_cov_4.380341, whole genome shotgun sequence.
ACCESSION   DAOSLL010000576 DAOSLL010000000
VERSION     DAOSLL010000576.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26649_length_3168_cov_4.380341"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(189..545)
                     /gene="rnpB"
                     /locus_tag="PLB25_19190"
     ncRNA           complement(189..545)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PLB25_19190"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            660..1577
                     /gene="cysK"
                     /locus_tag="PLB25_19195"
     CDS             660..1577
                     /gene="cysK"
                     /locus_tag="PLB25_19195"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HOE43733.1"
                     /translation="MRADNVLQTIGNTPHIRINRLFGNNHQVWVKSERSNPGGSIKDR
                     IALAMVEAAERSDVLQPGGTIIEPTSGNTGVGLALVAAVKGYKLILVMPDNMSIERRL
                     LMQAYGASLDLTPGDKGMKGAIARAYALSLTIRGSWVPQQFDNPANIQVHALTTAKEI
                     LADFPDGIDVLITGVGTGGHLSGVAQVLKAKWPRLKVYAVEPSASPVISGGASAPHAI
                     QGMGAGFVPKNLKTDLLDGVILVDAGPAFEYARRSATEEGLLVGISSGATLAAIAQKL
                     PEIAAGSTVLGFNYDTGERYLSVDGFLTA"
     gene            1844..1993
                     /locus_tag="PLB25_19200"
     CDS             1844..1993
                     /locus_tag="PLB25_19200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43734.1"
                     /translation="MPVEESHPQQALLLFDGQRAQRLMRNMSLASMAPGPHISNPHPE
                     HKLYR"
     gene            complement(2042..2629)
                     /locus_tag="PLB25_19205"
     CDS             complement(2042..2629)
                     /locus_tag="PLB25_19205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HOE43735.1"
                     /translation="MNWMRVLGRYKGLLVVCCFGLALLTIAQLTGMRDSVSLAFLRQT
                     LSEHRWGGLLAFVMLFCLGNLAQIPGWIFLASAVLVFGRSVGGMVTYISAVTSCAITF
                     LTVRFMGGAALCQFDNKVATRLLDQLQAHPVRNIIILRTIFQTLPALNFTLALSGTGF
                     RNYMMGTLLGLPLPIAAYCLLFDYLGVLVSAAGQR"
BASE COUNT          740 a          930 c          883 g          615 t
ORIGIN      
        1 atgcagtcgg tcaccctgcg cagtttggtt ctggagcaat tggccaaggg cgtcaaggtc
       61 gagcagcgac ccgcgcccaa ccgcggtgtg ttcaagaagc cggcacagca ggttgtgcgc
      121 accacggccc cgaactcggt ttttgccttg ggccagtcgc tgctggcgcg cggctgatcc
      181 gataaaaagt gtgaagccca ccgataagcc ggattctgtg cgtcagggtt gcccccaacg
      241 tgactgccat taatctgggc cactgatcac tcagtggctc ggtgctacct acccgccagc
      301 tccgggggcc ccatcaacac tggcctactt ggtattgctg cgcgtagaga ttgcccgttt
      361 cacccgctag ccggttgccc ggttgcgact cgtctctgtt gctctgatcc tcaccttata
      421 gccgcctacc tgcgcctgcg gtctttcagt ggacagccgt tagctgctac gctgccctgt
      481 gcagtccgga cgttcctcca gtgccgtgtt tccacaaatg caccagcggc agtctggcag
      541 gcttcaccca accattatcg cggcaaccac gggcaggtcc gcagcggcat cccagtaaac
      601 gtgacaatct gcgctctgtg caagcaaaaa aagcagtcat ttgaatttcc ggagacacca
      661 tgagagccga caacgtcctg cagaccattg gcaacacgcc acacatccgc atcaaccgcc
      721 tgtttggcaa caaccaccag gtctgggtca agtccgagcg cagcaacccc ggcggctcaa
      781 tcaaggaccg gattgcgctg gccatggtgg aagccgcaga acgatcggat gtgctgcagc
      841 ccggtggcac catcatcgag cccacctccg gcaacaccgg ggtcggactg gcgctggtag
      901 ccgcagtcaa gggctacaaa ctgattttgg tgatgcctga caacatgagc atcgagcgac
      961 ggctcttgat gcaagcttat ggtgccagtc tggatttgac ccctggtgac aaaggcatga
     1021 aaggggccat cgcccgcgct tatgcgctgt cgctgacgat ccggggctcg tgggtgccgc
     1081 agcagtttga caacccggcc aatatacaag tacatgcgtt aaccacggca aaggaaatac
     1141 tggccgactt cccggacggt atcgatgtgc tgatcaccgg ggtgggaacc ggcggccacc
     1201 tcagcggtgt ggcgcaggtg ctcaaagcca aatggccccg gttgaaggtt tatgcggtgg
     1261 agccttcggc ttcgccggtc atctctggcg gtgcgtcagc accacacgcc attcaaggca
     1321 tgggtgccgg ttttgttccg aaaaacctga agaccgatct gctcgatggt gtgattctgg
     1381 tcgatgccgg acccgccttt gagtacgccc ggcgcagcgc aaccgaagaa ggtttgctgg
     1441 tgggcatttc cagcggcgca accctggccg ccattgcgca aaaactgccg gaaattgcag
     1501 cgggctccac cgtgctgggg ttcaactacg acactggcga gcggtatttg tcggtcgatg
     1561 gtttcttgac tgcctgagcg caagtgttgc ggccacctcg ccagaattca atttccatca
     1621 caaagttaag tccaccggct cctgaagaga ccctggtttc cagacagatg tccgcgatgg
     1681 tttaagctgg acactgtcaa aaaaggggga tacaaacgag cgagagagaa aagcgcaaac
     1741 tgctcgaatc agcattcaag gccaaggtgg gtctggcagc ggtgcttgag gcgatgaccg
     1801 tcaacgaagt tggtcgggca cgcgaggtgc atccggtgcc ggtatgccag tggaagaaag
     1861 tcacccgcaa caagccttgt tgctgtttga cggccagcgc gctcagcgac tgatgcgaaa
     1921 catgagcctg gcaagcatgg cacccggccc ccacatcagc aacccgcatc cagagcacaa
     1981 gctataccgc tagttgctcc gtgccaacga cgccagttcc attcacggca ttccaggagc
     2041 ctcaacgctg gcctgcggca gaaacaagaa ccccaaggta gtcaaacagc agacagtagg
     2101 cggctatcgg tagcggcaga ccgagcaaag tgcccatcat gtagtttcta aaaccggtcc
     2161 ctgacaatgc cagtgtgaaa ttcagcgctg gcagtgtctg gaagatggtt cgaagaatga
     2221 tgatgttgcg caccggatgg gcttgcaact gatcgagcag cctggtggcg actttgttgt
     2281 cgaactgaca aagcgcagca ccgcccatga acctaaccgt caaaaacgtg atggcgcaag
     2341 acgtgacggc cgaaatgtag gtgaccatgc cgccaacgct ccggccaaac accaacaccg
     2401 ccgaggccag aaagatccat ccgggaattt gggccagatt gcccaaacaa aataacatca
     2461 cgaacgccag cagaccgccc cagcgatgtt ctgacagcgt ttgcctcaga aaagccaggc
     2521 tgacgctgtc ccgcatgccg gtcagttgcg cgatggtcag caacgccagc ccaaaacaac
     2581 agacaaccag caaacctttg taccgcccca agacgcgcat ccagttcatc atcgattcct
     2641 gaaacaaatt tccttttcga aatcattcgt gtctagcaca ctggcgcaga catcgcaaac
     2701 gcaagacaag gcaagacaac aacgacatgg atgattcaga aatggaataa gacaaaagcc
     2761 acacggcagg ggcgtactgg gcagtacgac gcagtcaggc gaaagcggat ccacgttggg
     2821 gatgtcgggc accagtgtaa tgacggattc atggcttccg tgatgtcatc gccgagccta
     2881 aaacacgtcc ctggtgcgca aggaccaggc atctgccgtg atcaatcgca gccatgattt
     2941 gccaaagcct gtaagaatct ggccgccagc cagactaaat gtcagccaaa gtcgagatta
     3001 cttcagtgat cgtcccgacc caatgctcaa gttgttgtca ctttcgcatt cacttttttg
     3061 tcttcaggag tatttcaaat gaaccaacgt gcccttattg ccagcgctgc tgtttccctg
     3121 atgtctttgg ccctggtggc tggcccggct gctgcagccg aaaaggaa
//
LOCUS       DAOSLL010000577         3166 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_26679_length_3166_cov_4.203150, whole genome shotgun sequence.
ACCESSION   DAOSLL010000577 DAOSLL010000000
VERSION     DAOSLL010000577.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26679_length_3166_cov_4.203150"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(98..1270)
                     /locus_tag="PLB25_19210"
     CDS             complement(98..1270)
                     /locus_tag="PLB25_19210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464611.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE43736.1"
                     /translation="MTSDTPPKKNWSWRSQAFRGLVYQLLAIIAIVGVVWFLAHNTLV
                     NMRVRGIQSGFDFLSQAAGFDIGESLYPFDSEQPYWQAFLVGVSNTLRVAVLGILLST
                     ILGTLLGVGRFSRNALVRGLCLSYVEFFRNIPVLLQLLMWYVVLTEVLPPASEAWSIG
                     SIFLSKGGLNYPIPVWAMGQLWAAVGLLAGVVLIWLYRRWAKARFEATGQLSSLFWVP
                     VLILIVCSLAGWALGGAPTELNHPTKGEFAIENGGALTPEFLAVLIGLTLYTASFIAE
                     VVRGGIASVPRGQGEAASALGLSQQQEMRLVMLPQALRVIIPPLTNQYLNLTKNSSLA
                     VAIGYPDVVSIANTALNQTGRAVECISLVMLVYLTTSLGTSLLMGWYNSRAAIKER"
     gene            complement(1392..2363)
                     /locus_tag="PLB25_19215"
     CDS             complement(1392..2363)
                     /locus_tag="PLB25_19215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HOE43737.1"
                     /translation="MPSHAGKTLDGIKARGTVICGVNTGLAGFGAADSAGKWTGLDVD
                     VCRAIAAATLGDPEKIKYVPLNAQQRFTALQSGEIDVLSRNTTFTLTRDASLGLNQTA
                     VTYYDGQGFMVPVKSKIKSAKQLKGQTVCVQSGTTTEKNLTDYSKAAGLGIKPVVFEQ
                     LEAAENAYFTGRCIAYTTDASGLASTRNKVAKVPADHIILPELISKEPLGPMVRRGDD
                     EWFAIVKWVIYGLLEAEEYSITQANVDSLKTSSADPVVKRILGTSEDTGKLLGLDKEW
                     MARAVKATGNYGEIFERNVGPKSALGLPRGVNNLWNKGGLMYAFPVR"
     gene            2676..>3166
                     /locus_tag="PLB25_19220"
     CDS             2676..>3166
                     /locus_tag="PLB25_19220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005216 - monoatomic ion channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HOE43738.1"
                     /translation="MARLEQELLGKPLAGWRLHLYTIIFEADTRLGRWFDQCLIAVIL
                     ASVAVVVADSVQAIAQSHHAMLNVAEWLFTLIFTVEYVARLVCVRRPLRYALSFYGVI
                     DLLALLPTYLALMVPEVGALIDVRVLRLLRVFRVFKLTAYVSEYQAIGQALRASQRKI
                     MVF"
BASE COUNT          682 a          881 c          926 g          677 t
ORIGIN      
        1 ggctttgatc caggccagcg ggccttcggt attgtttggg gccgggcgcg cggcaatcgg
       61 ttgaaaggtg cttgcattca tggtgtttgc ccttgcttca acgctctttg atggcggcgc
      121 ggctgttata ccaacccatc agcagggagg tgcccagcga tgtcgtcagg taaaccagca
      181 tcaccagcga aatgcactcc acggcgcggc cggtctggtt cagcgcggtg ttggcaattg
      241 acaccacatc cggataacca atggcaactg ccagcgacga atttttggtc agattgaggt
      301 actggttggt cagcggcggg atgatcacgc gaagcgcttg cggcaacatc accaggcgca
      361 tttcctgctg ctgcgacagc cccagggccg aagcggcttc gccttgaccg cgcggcacag
      421 acgcaatgcc accgcgcacc acttcggcaa tgaaggaggc ggtgtagagc gtcaggccaa
      481 tcagcacggc cagaaattcg ggtgttagcg cgccgccgtt ttcaatggcg aattcgcctt
      541 tggtcgggtg gttgagttcg gtcggggctc cgcccaacgc ccagcccgcc aggctgcaga
      601 cgatcaaaat caataccggc acccagaaca ggctggacaa ctgtccggtc gcttcaaacc
      661 gggctttggc ccagcgacgg tacagccaga tcagcacgac accggcaagc agacccactg
      721 cagcccataa ctggcccatc gcccagaccg gaatcgggta attcagtcca cccttgctca
      781 aaaaaatcga gccaatcgac cacgcttcag atgccggggg cagcacctcg gtgagcacca
      841 cataccacat cagcaactgc agcagcaccg gaatatttcg gaaaaactcg acatacgaca
      901 agcacagacc gcgcaccagt gcattgcgcg agaagcgacc gacccccagc agggtaccga
      961 ggatggtgga cagcaaaata cccagtactg caacacgcag cgtgttgctg acaccgacca
     1021 gaaatgcctg ccaatagggc tgctccgaat caaaggggta aaggctttcg ccaatgtcaa
     1081 aacctgctgc ctgggacaag aaatcaaagc cactctggat gccgcgcacc cgcatgttga
     1141 ccagcgtgtt gtgggccagg aaccagacca cgccaacgat ggcaatgatt gccagcaatt
     1201 ggtagaccaa gcctctgaaa gcctggctgc gccaggacca gttctttttg gggggagtgt
     1261 ctgaagtcat tgtgctgggt gccctgaggt acctgaatga gtaacacgaa gtgcatcggt
     1321 tggggatgca aatgaaaagc gccgcgtcaa tcgctggtgc gacatcgggc ggcgcgatca
     1381 ggaaggcggg gttagcgcac cgggaaggcg tacatcaagc cgcccttgtt ccacaggttg
     1441 ttgacaccac ggggcaggcc tagcgctgat ttggggccga cattgcgctc gaaaatctca
     1501 ccgtagttgc cagtggcctt gaccgcacgg gccatccatt ccttgtccag accgagcaac
     1561 ttgccggtgt cctcggaagt acccagaatg cgcttgacca ccgggtcggc gctggaagtc
     1621 ttgagcgaat ccacattggc ctgggtaatg ctgtattcct cagcttcaag cagaccgtaa
     1681 atcacccact tgacaatggc aaaccactcg tcgtcacccc ggcgcaccat cggcccgagc
     1741 ggctctttgg agatcagctc tggcaagata atatggtcgg cgggtacctt ggcgaccttg
     1801 ttgcgcgtcg aggccaggcc ggatgcgtca gtggtgtagg caatgcagcg accggtgaaa
     1861 taggcatttt cagcggcttc gagctgctcg aaaaccaccg gcttgatgcc cagaccagcc
     1921 gctttggagt aatcggtcag gttcttttcg gtggtggtgc cggactgcac gcagaccgtt
     1981 tggcccttga gctgcttggc gctcttgatc ttgctcttga ccgggaccat gaagccctgg
     2041 ccgtcgtagt aggtgacggc ggtctggttc agtcccagcg atgcatctcg cgtcagggtg
     2101 aaggtggtgt tgcgcgagag cacatcgatt tcaccggatt gcagggcggt aaagcgttgc
     2161 tgggcgttca gcggcacata cttgattttt tccgggtcac ccagcgtggc tgccgctatg
     2221 gcacggcaca catccacatc cagtcctgtc catttgccag ccgaatctgc cgcaccaaag
     2281 ccggccagac cggtgttgac tccgcagatc acagtgccgc gggctttgat gccgtccagg
     2341 gtcttgccag cgtgagatgg cattgccgta agagtaccaa gcgccgctgc aacgacagcg
     2401 attattttga atttgctaaa acgcattcgt gaaactccag taagaaaaga aatttaagcc
     2461 gtgatgatga aaccaacaga aaattcatca agaatcgaac tgtacagtgg ctggcgagtc
     2521 gctgcatccg ggtttactct tgaactttat gccattgact gataagttaa ttcgctctgt
     2581 ccgcaggaac gggacatgca ttaaacgtgc caggctgttg tgcatggcat cggccaaatt
     2641 cttttttttc agcgcgcaag cgatgcaggc aattgatggc caggcttgag caagagttgc
     2701 ttggcaagcc gctggcaggt tggcgactcc atctctacac catcattttc gaagcagata
     2761 cgcggctcgg acgctggttt gatcagtgtt tgattgcagt cattctggcc agcgtggcgg
     2821 tggtggtggc agacagtgtt caagccattg cgcagagcca tcacgccatg ctcaatgttg
     2881 cagaatggct ttttaccctg atttttacgg tcgagtatgt tgcgcggctg gtttgtgtgc
     2941 gccgtccgct acgctatgcc ttgagctttt acggcgtgat tgatctactg gccttgctgc
     3001 caacttatct ggcactgatg gtgcctgaag tgggggcctt gatcgacgtt cgagttctgc
     3061 gcctgctgcg cgtatttcgg gttttcaagc taacggccta tgtgtcggaa taccaggcga
     3121 ttggtcaggc gctgcgggcc agccaacgca agatcatggt cttttt
//
LOCUS       DAOSLL010000578         3158 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_26757_length_3158_cov_4.031260, whole genome shotgun sequence.
ACCESSION   DAOSLL010000578 DAOSLL010000000
VERSION     DAOSLL010000578.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26757_length_3158_cov_4.031260"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1238
                     /gene="ruvB"
                     /locus_tag="PLB25_19225"
     CDS             171..1238
                     /gene="ruvB"
                     /locus_tag="PLB25_19225"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HOE43739.1"
                     /translation="MSIQTDDFSAAPVKRMLSGAPASPNEQAIERALRPKLLDEYVGQ
                     AKAREQLEIFIAAAKMRNEALDHVLLFGPPGLGKTTLSHIIAHELGVNMRQTSGPVLE
                     KPKDLAALLTNLEKNDVLFIDEIHRLSPVVEEILYPALEDYKIDIMIGEGPAARSIKL
                     DLQPFTLVGATTRAGMLTNPLRDRFGIVARLEFYTPEELALIVKRSAGLLNVPTDEKG
                     GFEIARRSRGTPRIANRLLRRVRDYADVKGKGEISLDIANLALAMLDVDPQGFDLMDR
                     KLLEAVIHRFDGGPVGLDNIAASIGEERETIEDVIEPYLIQQGYLQRTPRGRIATLAA
                     YRHLGVTPPTSQPGGADMFSP"
     gene            complement(1448..1672)
                     /locus_tag="PLB25_19230"
     CDS             complement(1448..1672)
                     /locus_tag="PLB25_19230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43740.1"
                     /translation="MRHLRAMPMGLLALLVAVSLGACAYLQHPRFGDYQQGERQTTNE
                     RSPHFADGVFHKLVTTPMLAGGGDSSLCPS"
     gene            complement(1683..1979)
                     /locus_tag="PLB25_19235"
                     /pseudo
     CDS             complement(1683..1979)
                     /locus_tag="PLB25_19235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            complement(2185..2898)
                     /locus_tag="PLB25_19240"
                     /pseudo
     CDS             complement(2185..2898)
                     /locus_tag="PLB25_19240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
BASE COUNT          660 a          888 c          944 g          666 t
ORIGIN      
        1 gtcgcgaagg tgcttgcgca gtgcagtgtt ttcatgttgg tgccggggcc aaaagctgac
       61 ccggaaatag tcgtttgtgg catcttatcg gcagatcgtg ccatcggctt gaacggcggg
      121 tgcatcttcg ggcgctatag tgccagtttt catctgtatc cgagcctgta gtgagcattc
      181 aaaccgacga tttttccgct gcacccgtca agcgcatgct gtctggcgcc ccggcgtcgc
      241 ccaatgagca agcgatcgag cgcgccctgc gacccaagtt gctggacgaa tacgtgggcc
      301 aggcgaaggc gcgcgagcag cttgaaatat tcattgctgc tgccaaaatg cgcaacgagg
      361 cgcttgacca tgtgctgctg tttgggccgc ccggcctggg caaaaccacg ctgagccaca
      421 tcatcgcgca cgagttgggc gtcaacatgc gccagaccag cggcccggtg ctcgaaaagc
      481 ctaaggatct ggcagcgctg ctgacgaatc tggaaaaaaa cgatgtgctg ttcatcgacg
      541 agattcaccg gctaagcccg gtggtcgaag aaattctgta cccggcactc gaagactaca
      601 agatcgacat catgattggt gaaggcccgg cggcacgcag catcaagctc gatttgcagc
      661 catttaccct ggtcggtgcc accacccggg ccggcatgct gaccaatccg ctgcgcgacc
      721 gctttggcat cgtcgcgcgg ctggagtttt acacgccaga agagctggcg ctgatcgtca
      781 agcgcagtgc cggtttgctc aatgtgccga ccgatgaaaa aggcgggttt gagattgccc
      841 ggcgctcgcg tggcacgccg cgcattgcca accgcctgct gcgccgggtg cgcgattacg
      901 ccgatgtcaa gggcaaaggc gagatttctc tcgacattgc caacctggcg ctggccatgc
      961 tggatgtcga tccacagggc tttgacctga tggaccgaaa actgctcgaa gcggtgatcc
     1021 accgctttga cggcggcccg gtcgggctcg acaacattgc cgccagcatt ggcgaggagc
     1081 gcgaaaccat tgaggacgtg attgagccct acctgattca gcagggctac ctgcagcgca
     1141 cgccacgcgg acgcatcgcc acgctggcgg cctaccggca tctgggcgtg acaccgccga
     1201 ccagtcagcc cggtggggcg gacatgttca gcccttgagt cggcttgagc gggctgcacc
     1261 ttttataaat caaaatgtgc tctagcccgc ttgctgtatg cacaaaaagc tatttattta
     1321 atagtgactg gtccccggta gcagatcgcc aggcttccct aggctgcgtg accggtgatg
     1381 tcgcctgctg ctggatcggt gccagcgtca ggccaccgaa tgtcgggccg tttcctcaag
     1441 cggaccatca ggatgggcag agagaactgt caccgccgcc cgccaacatc ggcgtggtta
     1501 cgagtttgtg gaacacgcca tcggcaaaat gcggcgagcg ctcattggtt gtttgccgtt
     1561 caccttgctg gtagtcgcca aacctgggat gctgcagata agcgcaagca cccagagaaa
     1621 cggcgaccag cagtgccagc agacccatcg gcatggcgcg caagtgccgc atcgcctggt
     1681 tttcaggcgg cgttgttgag cgatgccatg tcgatcacaa agcggtagcg cacgtcgctc
     1741 ttgagcatgc gttcatatgc cctgttgatg tcttgcatcg ggatcgtttc gatgtcggaa
     1801 acgatgccat gcgcggcgca gaaatcaagc atttcctggg tttcgcggat gccgccaatc
     1861 aaggaacccg caatgctgcg gcgcttgagg atcagcgcga acacgctcgg cgatggatgc
     1921 ggctcggccg gtgcgccaac cagcgtcatc gtgccatcgc gcttgagcaa ttgaagatag
     1981 tgtctgaacg aaaaccccat ttgttaatat aaatcaacaa gttgcatcaa ttttttgaaa
     2041 cgaagatttt gctcaaaatt ttgttttccc tagggcgatt gtctgaaagt cgcgaaaaca
     2101 gaccgttttt cagaaatctt cgttttagaa aagtgatcta acaatatgat ttttataaga
     2161 aaaatgactt ttcgttcaga cacgaagata ggtgtccaac tggtgcggca cggcgacggt
     2221 gttgaggatg aaatcaagct gaccggcatg tcgctccaac tgcgccgggt cggtggaaac
     2281 gcacacctca tcagcaccga ggcgcaagcc gtcggcaact ttagcgggcg aggtggtgaa
     2341 cagcaccaca tgagctccca ttgcgtgggc cagcttgaca cccatatggc cgagtccgcc
     2401 cagcccgacg acaccgacct tcttgcctgg ccccacgccc cagtgacgca acggcgaata
     2461 ggtggtgata ccggcacaca gcaatggcgc gacaccggct tgatctttct ctgcatgggt
     2521 gatgcgcaag acaaacgact ccttgaccac aatcgactgg gcgtaaccgc catgggtgtt
     2581 gtcgcccccg agcagcggcc cgttataggt gccggtaaag ccgttgtcgc aaaactgttc
     2641 ctcgccctcg tggcacggcg ggcagtggcc gcaactgtcg accatgcagc cgacaccgac
     2701 cagatcaccg gacttgaacc gggtcacttg ttcgccaacg gcgaccaccc ggcccacgat
     2761 ctcgtggccg gggaccgacg ggtagagcgt gttctgccat tcgttgcgcg cagtgtgcag
     2821 gtcggaatgg cagacaccgc agtaagtgat gtcgatctga acgtcatctg cgcgcagtgc
     2881 gcgtcgcgtg atggccatcg gttcgagtgg tttgtcgctg gcatgggtgc cgaatgcctt
     2941 gacggtggtt gaatgattgg cgtgcatgca ctgttccttg gctgttgaag ttgaagtcgg
     3001 ccattgttac tcgcttgcct gggcttgagt agccaatgaa cgtttgacca agccattcaa
     3061 ggtcaatgtg ctgttgcaag cgcccgattt ggtgtgggtt gttgcgctaa ggatgtggtc
     3121 gggaataact ccccgttttg caccaaggag ttgggatg
//
LOCUS       DAOSLL010000579         3148 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_26870_length_3148_cov_3.995150, whole genome shotgun sequence.
ACCESSION   DAOSLL010000579 DAOSLL010000000
VERSION     DAOSLL010000579.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3148
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26870_length_3148_cov_3.995150"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="PLB25_19245"
     CDS             <1..833
                     /locus_tag="PLB25_19245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014725601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HOE43741.1"
                     /translation="VMTAIALLFGIHMVMAIGGADMPVVVSMLNSYSGWAAAATGFML
                     SNDLLIVTGALVGSSGAILSYIMCKAMARSFISVIAGGFGTASAAPAPSGGPAQPAGE
                     VFPIKAAETAELLREAKSVIIVPGYGMAVAQAQHTVYEITKFLRAKGCNIRFGIHPVA
                     GRMPGHMNVLLAEAKVPYDIVFEMDELNDDFPKTDVVMVIGANDIVNPGAQDDPNSPI
                     AGMPVLEVWKAKTSIVMKRSMASGYAGVDNPLFYKENNRMLFGDAKKMLDEVLVALKS
                     "
     gene            1003..2676
                     /locus_tag="PLB25_19250"
     CDS             1003..2676
                     /locus_tag="PLB25_19250"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HOE43742.1"
                     /translation="MTDRVWLKAYPRGVPADIDVSRYGSMVEMMEESFQKFADQRAFS
                     FLGKEISYAQVEELSRTFGVYLQGLGLVKGDRVAVMLPNVMQYPIAVAGILRAGFTLV
                     NVNPLYTARELEHQLKDSGSKVIVILENFAATLEKCLGATPVQHVVLCAMGDLLGLLK
                     GSIVNYVVRNVKKMVPEYKLPLAVRFNAAIAKASRGTLKKATLKPDDVAVLQYTGGTT
                     GVSKGAVLLHRNLVANLLQADAWFQPALTKIPAGEQMTTVCALPLYHIFAFTVGMMMS
                     MRLGGKLILIPNPRDFPAVLTELAKHKIHFFPAVNTLFNGLANHPDFSKVDWRSLVAS
                     AGGGTAVQSAVAKLWFEKTGCAICEGYGLSETASIAAANPVTATEYSASIGVPISSTW
                     LKLLDDDGNEVAAGQSGEIAIKGPQVMAGYWQRPDETAKVMTADGYFKSGDIGVMDAN
                     GFFRIVDRKKDMILVSGFNVFPTELEDVVTQMNGIMECACVGVPDQKSGEAVKLVIVR
                     KDPAITEDQVRAYCKENLTGYKQPKIIEFRTELPKTPVGKILRRELRVK"
     gene            2689..>3148
                     /locus_tag="PLB25_19255"
     CDS             2689..>3148
                     /locus_tag="PLB25_19255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HOE43743.1"
                     /translation="MTLAILSALLDEQRGLLEQLVNPQKVKRAGRTFWCGQWGGHDVV
                     LVLTKVGKVAAATTATTLIEAFGVTRVLFTGVAGGIGDGVKVGDVVVASAFVQHDMDS
                     SPLFARYEIPLYGCATFACDAAMTAMLLEATEAGVAGAGDHFHHQVLIDSA"
BASE COUNT          687 a          850 c          924 g          687 t
ORIGIN      
        1 tggtgatgac ggcgattgcg ctgctgttcg gcatccacat ggtgatggcc attggtggtg
       61 ccgacatgcc ggtggtggtg tcgatgctca acagctactc gggctgggcc gctgcggcca
      121 ctggcttcat gctgtcgaac gacctgctga tcgtcactgg tgccctggtc ggctccagcg
      181 gcgcgattct gtcttacatc atgtgcaagg cgatggcgcg cagctttatc agcgtgatcg
      241 ccggtggttt tggtaccgcc agcgcagcac cggctcccag cggtgggcca gcccagcccg
      301 ccggtgaagt attccccatc aaggcggcag aaactgccga actgctgcgt gaagccaaga
      361 gcgtgatcat cgtgccgggc tacggcatgg cggtagcgca ggcgcaacac acggtctatg
      421 agatcaccaa gttcctgcgt gccaaggggt gcaacatccg ctttggcatc catccggtgg
      481 ccggtcgcat gccaggccac atgaacgtgc tgctggccga ggccaaagtg ccctatgaca
      541 tcgtgtttga aatggacgaa ctcaacgatg actttccaaa aaccgatgtg gtgatggtga
      601 ttggtgccaa cgacatcgtc aatcccggtg cgcaggacga cccgaacagc ccgattgccg
      661 gcatgccggt gctggaagtc tggaaagcca agacctccat cgtcatgaag cgcagcatgg
      721 cttccggcta tgcgggtgtt gataacccct tgttctacaa ggaaaacaat cgcatgttgt
      781 tcggtgatgc caagaagatg cttgacgaag tgctggtcgc gctgaagtcc tgatcccgca
      841 ccctgtttgc tgcggccgcc ttgcgtggcc ccagtgggca ggcggctttc atgttttgag
      901 accggcaagt tggtattgcc tcactaatta gggaaaacac agcttacaaa aactgacatg
      961 caggtgaaaa tcaggttttg actaaaaact tggagaaaat cgatgactga tcgtgtctgg
     1021 ttgaaagctt atcccagggg tgtccccgca gacattgacg taagccgcta cgggtcaatg
     1081 gtcgagatga tggaagagag ctttcagaag ttcgccgacc aacgtgcatt tagttttctg
     1141 ggcaaggaaa tcagctacgc tcaagttgag gaactcagcc ggacctttgg cgtttacctg
     1201 caaggccttg gcctggtcaa aggcgaccgg gttgccgtga tgttgcccaa cgtgatgcag
     1261 tacccaattg ccgttgccgg tatcctgcgg gccggtttca cactggtcaa cgtcaatccg
     1321 ctgtacaccg cccgcgagct ggagcatcag ctcaaggact ctggctccaa agtcattgtg
     1381 attctggaaa actttgcagc aacgctggaa aaatgcctgg gtgccacccc tgtccagcat
     1441 gttgttttgt gcgccatggg cgatctactt ggtctgctca aaggttcgat agtgaactat
     1501 gtggtgcgca atgtcaaaaa aatggtgccg gaatacaagc tcccgctggc tgtccggttc
     1561 aacgcagcca tcgccaaagc cagcagaggc acgctcaaaa aggccactct caagccagac
     1621 gacgttgctg tcttgcagta caccgggggc accacgggtg tatccaaagg cgcggtcctg
     1681 ctgcaccgca atttggtggc caacctgttg caggcagacg catggtttca gcccgcttta
     1741 accaagattc cggcaggcga gcaaatgacg acagtgtgcg cgctgccgct ctaccacatc
     1801 tttgccttta ccgtcggcat gatgatgtcg atgcgtctgg gcggcaagct gatcctgata
     1861 cccaacccgc gcgactttcc ggcggtactg accgaacttg ccaagcacaa gatccatttt
     1921 ttccctgccg tcaacacgct gttcaacggc ttggccaatc accccgattt cagcaaggtg
     1981 gactggcgca gtctggtggc atcagccggt ggtggcaccg ccgttcaaag cgctgttgcc
     2041 aagctatggt ttgaaaaaac cggctgtgcc atttgtgaag gctatggctt gagcgaaacc
     2101 gcgtcaattg ccgccgcaaa cccggttacc gcgaccgagt attccgcctc gattggtgtg
     2161 cctatttcca gtacctggct caagctgctg gacgacgatg gcaacgaggt tgcagccgga
     2221 cagtcaggcg aaatcgccat taaggggccg caggtgatgg ctggctattg gcaaaggccc
     2281 gacgagaccg ccaaggtgat gacggctgat ggctatttca agtctggcga catcggggtc
     2341 atggatgcaa acggcttttt caggattgtt gaccgcaaaa aagacatgat cctggtcagc
     2401 ggcttcaatg tgtttccgac cgaacttgaa gacgtggtga cgcaaatgaa cggcatcatg
     2461 gaatgtgcct gcgtcggggt acccgatcag aaatccggcg aagcagtcaa gctggtgatt
     2521 gtgcgcaaag acccggccat taccgaagac caggtacgcg cctattgcaa ggaaaacctg
     2581 accggttaca agcaacccaa gatcatcgaa tttcgcaccg agttgcccaa gacaccggtg
     2641 ggcaaaatcc ttcggcgcga attgcgcgtc aaataaaaaa ggaaagattt gacactcgct
     2701 atcctgagtg ccctgcttga tgaacagcgg ggtctgcttg aacaactggt taacccgcag
     2761 aaagtgaaac gggcaggtcg taccttctgg tgtggtcaat ggggcgggca cgatgttgtg
     2821 ctggttctca ccaaggttgg caaggttgcc gctgccacca ccgccaccac gctgattgaa
     2881 gcctttggag tcacgcgggt gttgttcacc ggcgtcgccg ggggaattgg cgacggggtc
     2941 aaagttggcg acgtggttgt tgccagcgcc tttgtccagc acgacatgga ctccagcccc
     3001 ttgtttgcac gctacgagat acctctgtac ggatgcgcca cttttgcctg cgatgctgcc
     3061 atgactgcta tgctattgga agctactgag gcaggtgtgg cgggggctgg tgaccatttt
     3121 catcatcagg tgttgataga ttcagcca
//
LOCUS       DAOSLL010000580         3128 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_27057_length_3128_cov_3.898471, whole genome shotgun sequence.
ACCESSION   DAOSLL010000580 DAOSLL010000000
VERSION     DAOSLL010000580.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27057_length_3128_cov_3.898471"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(303..1622)
                     /locus_tag="PLB25_19260"
     CDS             complement(303..1622)
                     /locus_tag="PLB25_19260"
                     /inference="COORDINATES: protein motif:HMM:NF021255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HOE43744.1"
                     /translation="MTNHQTPSPHHGAVEIAINWLSSLHRKGWQNASINLYQELLSEE
                     ERVRVAGLDQDTVEAIEINMVDWLLAEGDIQARGGIRRINDYLLSPAGPRLNAGQRDW
                     LQQMGQRPLRLYDVTDVVKSQQMTLCDALDKEAEPLVVRERMGTQNLEPGSLLACRIV
                     RAGDHFEMSGSAYLFGLLTGPGVLSRLREHQQAHGQQLDGPRRMGRMIMREWVQQWLV
                     PAPMPTLIDHYSGEPMKMITDHYRVNDEMALAQTLATQPDIEGDRQEGWSRLIDCTDG
                     QIRASVHINPGKKPDQIELFYRTQRYADDGREWFEALVGASVTFVKRKAQETADVMAA
                     PQKPAARGSSKKGKPSSASASTLDAATMAQLMSEAIHRSYANWADEPIPALDHKTPRQ
                     AIKTSAGLERVKGLLRSYEASEAAQAKQQGRTEISYDFLWQALGLTR"
     gene            complement(2133..2498)
                     /locus_tag="PLB25_19265"
     CDS             complement(2133..2498)
                     /locus_tag="PLB25_19265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE43745.1"
                     /translation="MEKKILVVDDADISRMIATRALKAAGYKVFEATNASNALALLDG
                     RDISMVVCDFSMPGMDGIEFVKALKEMSQYKLMPVLMLTAADKQEIRERGKKSGVKAW
                     MTKPFSQPALVKAVDKLCP"
     gene            complement(2581..2973)
                     /locus_tag="PLB25_19270"
     CDS             complement(2581..2973)
                     /locus_tag="PLB25_19270"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOE43746.1"
                     /translation="MEMKSDMSASSEQAPVDFERSLKGLGDDGKLLEAVAQMLLEQIT
                     VDLTAIQSNVAASNSCALADASHHLKGSLGAIAAVPAYLACAALNRSARCGAIASYLP
                     ELARLEHELTRLRRCLQVWLIEQEKCGS"
BASE COUNT          647 a          898 c          823 g          760 t
ORIGIN      
        1 accaggacta tgtttgatga aggtcactgc gaatgccatc tgattttcaa gccctattgg
       61 cctgtagccc ttgtaaatag tgcgcaagca gctatatatt tgatagtctg ttgaggctga
      121 tcgccgaact gaccaagacc atgccgccag cctttgactg catcatcatc ggtgctggct
      181 ccagcggctg cgtgttggtg ggccgtctga gagaacaccc agccatgcgg gtgttgctgc
      241 gagaaggtcg ggccagcccg ccgatctgtc cggcccgaaa ttgtgaccaa ccgtctggcc
      301 agctatcggg ttaaacccag cgcttgccac aaaaagtcat acgagatttc agtgcgaccc
      361 tgttgcttgg cctgcgctgc ttcactggcc tcataactgc gcaacaggcc tttgacccgc
      421 tccagaccgg ctgaggtctt gatggcctgg cgcggtgttt tgtggtcaag cgccggaatc
      481 ggttcatcgg cccagttggc atacgagcga tgaatggctt cggacatcaa ttgcgccatg
      541 gtggcggcat ctagtgtgga cgcagatgct gaagacggct tgcccttttt gcttgagccc
      601 ctggccgccg gcttttgcgg tgcagccatc acatccgcag tctcttgcgc ttttcttttg
      661 acaaaggtga cacttgcgcc caccaacgcc tcaaaccact cccgcccatc gtcggcatag
      721 cgttgtgttc gataaaaaag ttcgatctga tcaggctttt tgccgggatt gatgtgcaca
      781 ctggcgcgta tctgcccgtc cgtgcagtcg atgaggcgtg accacccctc ttgccgatcg
      841 ccctcaatat ctggctgggt ggccagggtt tgcgccagcg ccatttcatc attgacacgg
      901 taatggtcgg tgatcatctt catgggctcg cctgaatagt gatcaatcag ggttggcatg
      961 ggtgcgggca ccagccattg ctgcacccat tcgcgcatga tcatccgccc cattcttcgg
     1021 ggtccatcaa gctgctggcc atgcgcctgc tggtgttcgc gcagccgcga cagcaccccc
     1081 ggcccggtta gtaacccaaa caaataggcc gagccagaca tttcaaaatg atcgccagcg
     1141 cgaacgatgc ggcaagccag caagctgccg ggctccagat tttgcgtacc cattcgctcg
     1201 cgcaccacca agggttcggc ttctttgtcg agcgcatcac acagtgtcat ttgttggctc
     1261 ttcaccacat cagtcacgtc atagaggcgc agcgggcgct ggcccatttg ctgcagccaa
     1321 tcgcgctggc ccgcgttcag acgcggacca gccgggctga gcaggtaatc attgatgcgc
     1381 cggatgccac cccgggcctg gatatcgccc tcggccagca gccaatcgac catgttgatt
     1441 tcaatcgctt caaccgtgtc ttgatccaat cctgcaaccc gcacccgctc ttcttctgaa
     1501 agcagttcct ggtacaaatt gatagaggcg ttttgccagc ccttgcggtg caaagaagac
     1561 agccaattga tggcaatttc aactgcaccg tggtgtgggg agggcgtttg atggttggtc
     1621 atggtgggcg cggtttggta ctcaaaagaa cgtagatttt cactgaattc tctgccgcca
     1681 gataggccgg acatgccagg caaccgcaga cttgagtttc ttctgcctaa gccggacaag
     1741 ccggaatcga tgagccttcg tcgcgagggc gcgactccta cacaaacagt cggcgcctat
     1801 ttgaatttgt aggcccgccc tcgcgacgaa tgacttggca tgtttgaatt cttcgcatga
     1861 ttcgcaaaga cttgacttga tagagactag gcttggttgg ttccagagca gcgtcagcgg
     1921 gtactggcca aagatagctt tccgaaagct cgacgaaatc tatctcggca gccgttcgtg
     1981 gtccacatct ggctgcccaa agtgggtgca aaggttgtgt gcgccgtcat ttaactcaac
     2041 gcatcaaaat gaaacgctgg ttctgaaatt cgcggcacta cagcggtcac agtcctgtga
     2101 agtttgtttc ttggatactg ccgcttcata gcctatggac agagtttgtc gactgctttc
     2161 accaatgccg gctgagaaaa tggcttcgtc atccacgcct tcacaccgga ctttttgcct
     2221 ctctcgcgta tttcctgttt gtcagcagct gtcagcatca ggaccggcat caacttgtac
     2281 tgggacatct ctttaagcgc cttgacaaat tcaattccgt ccatccctgg catgctgaaa
     2341 tcgcaaacga ccatgctgat atctcgccca tcgagcaagg cgagcgcgtt gctcgcgtta
     2401 gtcgcttcaa agaccttgta accagcggct ttgagggccc tggttgcaat catgcgcgat
     2461 atgtcggcgt cgtctacaac gagaattttc ttttccatga tggaaactcc aaatgtttag
     2521 atcctctaat tactggcacg tcggtgcgca ctcttcggtt ccagcggtct gacttcctgc
     2581 tcacgacccg cacttttctt gctcgatgag ccagacctgc agacaccgtc ggaggcgggt
     2641 taactcatgc tccagtcggg ccaattctgg taaatacgat gcaatcgcgc cacatcgcgc
     2701 tgaccggttg agtgcagcac atgccaggta ggcaggcacc gcagcgatgg ctcccaagct
     2761 acccttaaga tggtgagacg catcagcaag tgcacaggaa ttgctggccg ccacattcga
     2821 ctggatagct gtcaagtcaa ccgttatctg ctcgagcaac atttgtgcga ctgcttcaag
     2881 aagtttgccg tcatctccga gccctttcag tgacctttcg aaatcgacgg gcgcttgttc
     2941 acttgaggca gacatgtcag acttcatttc catcaatact ccctggactg acagcgaata
     3001 atgcaatccg ctgctagttc ttgtgccgcc tttcgctgtg gcgcatcaac caagaacgtc
     3061 agatcgttca tcttgagcga caggtgggtc agtgcgtcgc gcatgacttc cagctctttc
     3121 accaattg
//
LOCUS       DAOSLL010000581         3125 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_27086_length_3125_cov_4.288274, whole genome shotgun sequence.
ACCESSION   DAOSLL010000581 DAOSLL010000000
VERSION     DAOSLL010000581.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27086_length_3125_cov_4.288274"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /gene="prfA"
                     /locus_tag="PLB25_19275"
     CDS             complement(<1..1093)
                     /gene="prfA"
                     /locus_tag="PLB25_19275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HOE43747.1"
                     /translation="MKTFLRQQLARYADRLGELEFLLSRQDIMSDMAQFLLLSREHTD
                     VAAVAGRWARYQQRESDLAAAQDLLKDAADDAEMAQMAQEEIDAASAEMQQLESELQR
                     MLLPKDPDDARPAFVEIRAGTGGDESALFAADLARMYLRFCDRKGWKHEIISESPSEL
                     GGYKELVLRIEGDQVYGALKFESGGHRVQRVPVTETQGRIHTSACTIAVLAEPDEQDA
                     IKINPSDLRIDTYRASGAGGQHINKTDSAVRITHLPTGIVAECQDGRSQHSNKAQALR
                     VLTARIQEKDRSERAAKDAAERKSLVGSGDRSDRIRTYNFPQGRLTDHRINLTLYKLL
                     TIMEGDLDDVVHALQAHEAANQLAALELNA"
     gene            complement(1568..2827)
                     /gene="hemA"
                     /locus_tag="PLB25_19280"
     CDS             complement(1568..2827)
                     /gene="hemA"
                     /locus_tag="PLB25_19280"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HOE43748.1"
                     /translation="MAVWALGINHTTAPLDLRGRFAFAMDQIEPTLRALRGALIRQPE
                     AALISTCNRTEIYCAGEQAQLQPTLDWLAHSGGVSAELLRAHSYTLQDGLAARHAFRV
                     ASGLDSMVLGEPQILGQLKDAVRAADAAGSLGSTLSQLFQRSFAVAKEVRSSTEIGAH
                     SISMAAAAVRLAGQLFEDLNQVKVLFVGAGEMIDLCATHFAAKTPKAMAIANRTLARG
                     ETIAAKFGAEVMHLGELPDRLHEFDAVISCTASTLPLIGLGSVERALKRRRRRPMFMV
                     DLAVPRDIEIEVKALEDVYLYTIDDLAGVVQTAQASRQAAVAQAEAIVDAGVQSFLHW
                     VEQRNTVPLIQQLNAQTEEWRALEIARARKLLAKGADVEEVLDALSRGLTQKMLHGAL
                     AELRGGDSAARERASSAVQHFFLRKER"
     gene            2898..>3125
                     /locus_tag="PLB25_19285"
     CDS             2898..>3125
                     /locus_tag="PLB25_19285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019702154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43749.1"
                     /translation="MGPLALLNHAINFMAPALWLALLIPLLARLLMRKRPVALTVLAQ
                     VAVLFVAGCGLLTAGLVLFGRDGKMLTYLAMV"
BASE COUNT          563 a          926 c          999 g          637 t
ORIGIN      
        1 aggcgttgag ttcaagcgcg gcgagctggt tggccgcctc atgcgcctgg agggcatgga
       61 ccacgtcgtc aagatcacct tccatgatgg tcagcagttt gtacagggtg aggttgatgc
      121 ggtgatcggt caggcggccc tgcggaaagt tgtaggtgcg aatgcggtca ctgcggtcgc
      181 cgctgccaac caggctttta cgctcggcgg catccttggc ggcccgctcg ctgcggtctt
      241 tttcctggat gcgggcggtc agcacgcgca aagcctgcgc cttgttgctg tgctggctgc
      301 ggccatcctg gcattcggcg acgatgccgg ttggcaaatg ggtgatgcgc accgccgagt
      361 cggttttgtt gatgtgctgg cccccggctc cgctggcgcg gtaggtgtca atgcgcaggt
      421 ctgacgggtt gatcttgatg gcatcttgct cgtcgggctc ggccagcacg gcaatggtgc
      481 aggcgctggt gtggatgcgg ccctgggttt cggtgaccgg cacacgctgc acccggtggc
      541 cacctgactc gaacttgagc gcgccataaa cctgatcgcc ttcaatacgc agcaccagtt
      601 ccttgtagcc accgagttcg ctgggagact cgctgatgat ttcgtgcttc cagcccttgc
      661 ggtcacaaaa acgcaggtac atgcgcgcca ggtcggcggc aaacagggcc gactcgtcgc
      721 caccggtgcc agcgcgaatt tcgacaaaag ccgggcgggc atcgtccggg tcttttggca
      781 gcagcatgcg ctgcagttcc gactccagtt gctgcatctc ggcgctagcg gcatcgattt
      841 cttcttgtgc catttgcgcc atctcggcat cgtcggcggc atccttgagc aaatcctgcg
      901 ccgcagccag gtcagattcg cgttgctgat agcgcgccca gcgacccgcc acggctgcca
      961 cgtcggtgtg ttcccgcgag agcagcagaa actgggccat gtcgctcatg atgtcctggc
     1021 gtgacagcaa gaattcgagt tcccccaggc ggtcggcgta gcgggcaagt tgttggcgca
     1081 aaaatgtttt catgatccta gattctttag ttggacgcgc ccgagtgggc cgctggcggc
     1141 gcgtctgggt agggcgaaag ccgcggcttc aaacctgcct gcgcaggttt ggacggcttg
     1201 agcaggccat gcttctggca tggctgcgcc ctgcaaggga gacgacgacg cacagttact
     1261 gcccgctagg aggagcaacg cccccaggcg cgttgccagt ggttcaatcg ggtgcgtagc
     1321 gctgtcaccc gatgggtgaa ctccatcgaa gctaaaaaag taaatactca taaggactta
     1381 tagaggaaaa caagcggtca actgaggaat ctaggatgat gtggtgaagg tgacgcgggg
     1441 gctggatcgg gtcggcttga agcggacaac tggtttgtgg tggccgttgc gcgcctggcg
     1501 cggcaatcgg ctttggcgga ctggtcgcgt cgtcgcgctg gcatttacgc tggtgcaatc
     1561 agcccagcta acgctctttg cgcaaaaaga aatgttgcac cgctgagctg gcacgctcgc
     1621 gcgcggcgct gtcgccgcca cgcaactcgg ccagcgcgcc gtgcagcatt ttttgcgtca
     1681 ggccacgtga cagggcatcg agcacctctt ccacgtcggc acctttggcc agcagcttgc
     1741 gggcccgggc aatctcaaga gcgcgccatt cttcggtctg ggcgttgagc tgctggatca
     1801 gcggcaccgt gttgcgctgc tcgacccagt gcaaaaagct ctgcaccccg gcatcgacaa
     1861 tggcttcggc ctgggctacg gcggcctgcc gactggcctg ggcggtttgc accacaccgg
     1921 ctaagtcatc aatggtgtag aggtaaacgt cttcgagcgc cttgacctca atttcgatat
     1981 cgcgcggcac tgccagatcg accatgaaca tcgggcgccg acggcggcgc ttcaaggccc
     2041 gctcaaccga gccaagacca atcaggggca gcgtgctggc ggtgcaactg atcaccgcgt
     2101 caaattcatg cagccgatca ggcagttcgc ccaggtgcat cacctcggcg ccaaacttgg
     2161 cagcgatggt ttcgccacgc gccagggtgc ggttggcaat ggccatggct ttgggggttt
     2221 ttgcggcaaa gtgggtggcg cacagatcaa tcatctcgcc agcgccaaca aacagcacct
     2281 tgacctggtt gaggtcttca aacaactggc cggccaggcg cactgccgct gcagccatgc
     2341 tgattgaatg cgcgccaatc tcggtcgagc tgcggacttc cttggccacg gcaaacgagc
     2401 gctggaacaa ctggctcagc gtgctgccca ggctgcctgc ggcatcggcg gcgcgcacgg
     2461 catccttgag ctggcccaga atctgcggct cgcccagcac cattgaatcg agcccgctgg
     2521 caacccgaaa ggcgtggcgc gcggccagtc cgtcttgcaa ggtgtaggag tgagcgcgca
     2581 gcaattcggc tgagacacca ccgctgtgcg ccagccagtc cagcgttggc tgcaattggg
     2641 cttgctcgcc agcacaataa atttcggttc ggttgcaggt ggaaatcagc gccgcctcgg
     2701 gctggcggat cagcgcaccg cgcagggcac gcaaggtggg ctcgatctgg tccatggcaa
     2761 acgcaaaccg gccacgcaag tcgagcggtg cggttgtgtg attgatgcct aatgcccaaa
     2821 ctgccatggc tgaattataa aatcaggcgc atgcgctttg aactgaatca ttcgttgttg
     2881 ccactgactt gtcattgatg ggaccgctgg cactgctcaa ccacgccatc aacttcatgg
     2941 caccagcgct gtggctcgcc ctgctgatcc ccttgctggc ccggcttttg atgcgaaaaa
     3001 ggcctgtagc ccttacggtg cttgcgcagg tagctgtact ttttgtagca ggatgcggtc
     3061 tgctgacagc cggactggtg ctgtttgggc gcgacggcaa gatgctcacc tacctggcca
     3121 tggtg
//
LOCUS       DAOSLL010000582         3120 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_27136_length_3120_cov_3.800000, whole genome shotgun sequence.
ACCESSION   DAOSLL010000582 DAOSLL010000000
VERSION     DAOSLL010000582.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27136_length_3120_cov_3.800000"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(96..1964)
                     /gene="glmS"
                     /locus_tag="PLB25_19290"
     CDS             complement(96..1964)
                     /gene="glmS"
                     /locus_tag="PLB25_19290"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004637163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HOE43750.1"
                     /translation="MCGIVGAVSVQNVVPIMVQGLARLEYRGYDSCGVAVYANGLQRA
                     RSTSRVAELMAQISTTQLAGSTGIAHTRWATHGAPVVHNAHPHFSHGPGADADSRPGH
                     IALVHNGIIENHDDLRALLQAKGYVFQSQTDTEVIAHLIDSLYDGDLFESVKAALGSL
                     RGAYAIAVFCKDEPHRLIGARVGSPLILGVGADGQSHYLASDAMALAGVTDQIVYLEE
                     GDVADLQLGKYWLVNAAFKPLTPAQRPVKTVHAHSGGAELGPYRHYMQKEIFEQPRAI
                     ADTLEGVTSIMPELFDGANASAARVFRDIDSVLILACGTSYYSGCTAKYWLESIAKIP
                     TQVEVASEYRYRDSVPNPRTLVVTITQSGETADTLAALRHAQGLGMTHTLTVCNVATS
                     AMVRECELVYVTRAGVEIGVASTKAFTAQLAGLFLLTLALAQSRGLLSDAEEARHIKA
                     LRHLPAALQAVLALEPQVIAWSEEFAGKDNALFLGRGLHYPIALEGALKLKEISYIHA
                     EAYPAGELKHGPLALVTSAMPVVTVAPNDALLEKLKSNMHEVRARGGVLYVLADAGSQ
                     IASGDGLHVIRMPEHYGELSPMLHVVPLQLLAYHTACARGTDVDKPRNLAKSVTLE"
     gene            2104..2994
                     /locus_tag="PLB25_19295"
     CDS             2104..2994
                     /locus_tag="PLB25_19295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOE43751.1"
                     /translation="MKTSLSLRHFFLALAVVAVWGSNFVVIKVALGQMPPLLFATLRF
                     CLVLLPMVFILPRPALPWRNLAAYGLLIGVGQFGLLFVAMNGHISPGLASLVIQVQVF
                     FTIGLAMAMAGETLQRVQWIALGLAGAGLWVIVAHTDGSTTLLGLGLVLLAALAWAGG
                     NLVSRAAGRINMVAYVVWSSLFAVPPLAVLALTVEGWDVLAAGLQQASAMTWAAVAWQ
                     AWGNSIFGYAAWGWLLSRYSAATITPMALLVPLFGMGSSAWWLRESLPGWKIGAAALV
                     MGGLALNLLWPWWVRKGRAA"
     gene            2991..>3120
                     /locus_tag="PLB25_19300"
     CDS             2991..>3120
                     /locus_tag="PLB25_19300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOE43752.1"
                     /translation="MNTPELDAIDLQLLARLQQDASMNNQTLAELVHISPPTCLRRV"
BASE COUNT          560 a          935 c          968 g          657 t
ORIGIN      
        1 gccaaaggcg aacctcattt ttccagcttg tctgggaaaa tgcctagtca ctaaccgcgc
       61 cattggcatt gaatgatcaa cagaggatac cggcatcatt ccagcgtgac cgatttcgcc
      121 aggttgcgcg gtttgtcaac atcggtacct ctggcgcagg cggtgtggta ggccagcaac
      181 tgaagcggca ccacatgcag catcggcgag agttcgccgt aatgttcggg catgcggatc
      241 acgtgcaggc cgtcaccgct ggcaatctgc gagccggcat cggccagcac atacaacacg
      301 ccaccgcggg cgcgcacctc gtgcatgttg ctcttgagtt tttccagcag cgcgtcgttg
      361 ggggccaccg tgaccaccgg catggcactg gtcaccagtg ccagcggccc gtgcttgagt
      421 tcacctgccg gataggcctc ggcatggatg tagctgattt ccttgagctt gagtgcgcct
      481 tccagcgcaa tcgggtagtg cagcccacgt cccagaaaca gcgcattgtc cttgcccgca
      541 aactcttctg accaggcgat cacctgcggc tccagcgcca gcaccgcctg cagggcggcc
      601 ggcaaatggc gcagggcctt gatgtggcgc gcctcttctg cgtcgctgag caggccacgc
      661 gattgcgcca gtgccagcgt cagcaagaac agcccggcca gttgtgcggt aaaggccttg
      721 gtggaagcaa caccaatttc aaccccggcg cgggtgacgt agaccagttc gcattcgcgc
      781 accatggcgc tggtggccac attgcaaact gtcagcgtat gcgtcatgcc caggccttgc
      841 gcatggcgaa gcgcggccag ggtatcggcg gtttcgccgc tttgggtaat ggtgaccacc
      901 agcgtgcggg ggtttggcac cgagtcgcgg tagcggtatt cgctggcaac ctcgacctgg
      961 gtcggaattt tggcgatgct ctccagccag tacttggcgg tgcagccact gtaatagctg
     1021 gtgccacagg ccagaatcag caccgaatcg atgtccctga aaacccgcgc cgcgctcgcg
     1081 ttggccccgt caaacagctc gggcatgatg ctcgtgacac cttcgagggt gtcggcaatg
     1141 gcgcggggct gctcaaagat ttccttttgc atgtagtggc ggtacggccc cagctcggca
     1201 ccgccactgt gggcgtgcac tgttttgacc gggcgttgtg ccggtgtcaa tggcttgaag
     1261 gctgcgttca ccagccagta cttgcccaac tggaggtcgg ccacgtcccc ttcttccagg
     1321 taaacaatct ggtcggtgac accggccaat gccatggcat cgctggccag gtagtgcgac
     1381 tgcccgtctg ccccaacccc cagaatcagc ggcgagccaa cccgcgcgcc aatcagccgg
     1441 tgtggttcgt ccttgcaaaa caccgctatg gcataggcac cacgcaacga gcccagcgcc
     1501 gccttgaccg actcaaacaa atcaccgtca tacaggctgt caatcaaatg ggcaatcacc
     1561 tcggtgtcgg tctgactctg aaaaacatag cctttggctt gcagcagggc acgcaggtca
     1621 tcgtggtttt caatgatgcc gttgtgcacc agggcaatgt ggccaggacg gctgtctgca
     1681 tctgcgccgg ggccatggct gaaatgcgga tgcgcgttgt gtaccaccgg cgcgccatgg
     1741 gtggcccagc gggtgtgggc tatgccggtg ctgccggcca gttgggtggt gctgatctgc
     1801 gccatcagtt cggccacccg cgaagtgctg cgggcgcgct gcaagccatt ggcatagacc
     1861 gccacaccgc aagagtcgta accccggtat tccagcctgg ccaggccttg caccatgatg
     1921 ggaaccacgt tttggaccga taccgcgcca acaatgccac acatagactg ctccaaaaaa
     1981 taaacaatgc ctgatgttag aaagcatgat cagaaattac tgatcaatat ttgcatacat
     2041 tcaaccaaat atttcaccaa acaaaacaag tcaccattta tttcaccaat ctatacttgg
     2101 tgaatgaaaa cttcattgtc tttgcgtcat ttttttctgg cattggcggt ggtagcggtt
     2161 tggggcagca attttgtggt catcaaagtg gcgctggggc agatgccacc cttgctgttt
     2221 gccaccctgc gcttttgcct ggtgctgctg cccatggtgt tcattttgcc cagaccggcg
     2281 ctaccctggc gcaatctggc cgcatacggg ctgctgattg gggtcgggca gtttggcttg
     2341 ctgtttgttg ccatgaacgg gcatatttcg cctggactgg cctcgctggt gattcaggtg
     2401 caggtgtttt tcaccattgg cctggccatg gcgatggccg gtgaaacgct gcagcgggtg
     2461 caatggatcg ccctggggct ggccggagcc ggtttgtggg tgatcgtggc gcataccgac
     2521 ggcagcacca ccctgctggg cctgggcctg gttttgctgg ccgccctggc ctgggctggc
     2581 ggcaatctgg tgtcgcgcgc ggccgggcgc atcaacatgg tggcttatgt ggtgtggtcc
     2641 agcctgtttg ccgtgccacc gctggctgtg ctggccttga cggtcgaggg ctgggacgtt
     2701 ttggcggccg gactgcagca ggccagcgcc atgacctggg ccgccgtggc ctggcaggcc
     2761 tggggcaatt ccatttttgg ctacgccgcc tggggctggc tgctgtcgcg ctacagcgca
     2821 gccaccatca cgccaatggc cttgctggtg ccgctgtttg gcatgggcag ttcggcctgg
     2881 tggctgcgcg aaagcctgcc tggctggaag attggcgcgg cggctttggt gatgggcggg
     2941 ctggcgctga acctgctgtg gccgtggtgg gtgcgcaaag gtagggcagc atgaacacgc
     3001 ccgagcttga cgccatcgat ctgcagctgc tcgcgcgcct gcagcaagac gcatccatga
     3061 acaaccagac cctggccgag ctggtgcaca tctcgccgcc cacctgcctg cgccgcgtca
//
LOCUS       DAOSLL010000583         3107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_27281_length_3107_cov_9.417431, whole genome shotgun sequence.
ACCESSION   DAOSLL010000583 DAOSLL010000000
VERSION     DAOSLL010000583.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27281_length_3107_cov_9.417431"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="PLB25_19305"
     CDS             complement(<1..210)
                     /locus_tag="PLB25_19305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007836316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HOE43753.1"
                     /translation="MTNAPATPYRKPPRPRSPQSHEVRIIGGQYKRTRLPVADKAGLR
                     PTPDRVRETLFNWLGQDLTGWRCVDA"
     gene            complement(421..1782)
                     /locus_tag="PLB25_19310"
     CDS             complement(421..1782)
                     /locus_tag="PLB25_19310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOE43754.1"
                     /translation="MKNIKSIASYALLICATSLFLINHAQAGIAIEHWTSPGGVQVYL
                     VPSPAIPMLDVQIDFDAGSRRDPPQQAGLASVTARMLAKGVRANPARQEPALDENALG
                     EAWADLGASFGASASSDRISFALRTLSYPDLLAKAVQLAARQLAEPSFPPDIWQRERD
                     SLKASLKEANTRPATLAARAFAKAVYGDHPYGYEMTEATLSRLEVADMAALHRQMVQA
                     CRASVTLVGAVTRPQADALVQELFARLSSSKQAGCAALPKVAEVAALEQASEQRIVFD
                     SAQAQVLLGQPGFKRDDPDYFALTVGNYILGGGGFVSRLTEQVRERRGLSYSVFSNFA
                     PGLHAGAFAISLQTRPDQADQALQVAREVLARFVADGPTAPELQAAKDNLIGGFALRI
                     DSNRKLLDNVANMAWNNLPLDYLDTWTQQVEGVTAAQIRAAFARKLQPQRMATVVLGG
                     ATK"
     gene            2218..2700
                     /locus_tag="PLB25_19315"
     CDS             2218..2700
                     /locus_tag="PLB25_19315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HOE43755.1"
                     /translation="MKRKIYVETSVISYLTARPSRTIIGAAHQQITLAWWETRSQYEL
                     LVSESVLRECGAGDPDAAKKRLAVLNDVPLLLVTEQALNIAESLVEREIIPLKAAEDA
                     LHIAVATVNGVDYLLTWNCRHIANPEIQRNIAQYLEQIGLFLPFICTPEELLGEENAE
                     "
     gene            2690..2896
                     /locus_tag="PLB25_19320"
     CDS             2690..2896
                     /locus_tag="PLB25_19320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43756.1"
                     /translation="MLNDEIIDEVRAIREAHASKFGYDLRAIYADLKKSEGKRIANGH
                     PYVSPPSEPPAPNSAFQRIRFAHR"
BASE COUNT          677 a          866 c          912 g          652 t
ORIGIN      
        1 cgcatcgacg cagcgccagc cggtcaggtc ctggccgagc cagttgaaca aggtttcacg
       61 cacccggtcg ggtgtcgggc gcagtcccgc cttgtctgcc accggcagcc tggtgcgttt
      121 gtactggcca ccgatgatgc gcacctcatg gctttgcggc gagcgggggc gcggtggctt
      181 acggtaaggg gtagcggggg cgtttgtcat ctggtaaggc gcgggatgga tagagcggca
      241 ttgtccatga ggccaagctt aggatgtggt cagaaataac ttcccctatt tgcaccggcg
      301 gatctgggcg cacagcgtcg ttgcaagacg cttgtttagc cagctaaacg gcgcgtcttg
      361 cgcctagccg tgcatcccaa ctccttggtg caaaacgggg agttattccc gaccacatcc
      421 ttacttggtg gccccaccca gcaccacggt ggccatgcgc tggggctgca gttttcgcgc
      481 aaaggcggcc ctgatctgtg ccgctgtcac gccttcgacc tgctgcgtcc aggtatcaag
      541 gtagtccagc ggcagattgt tccaggccat attggccacg ttgtcgagca gtttgcggtt
      601 gctgtcgatg cgcagcgcaa agccaccgat caggttgtct ttggcggctt gcaactcagg
      661 cgcagtcggg ccgtctgcga caaaacgcgc cagcacctcg cgtgccacct gcaacgcctg
      721 atcagcctgg tccgggcggg tttgcagact gatggcgaaa gcaccggcat gcaggccagg
      781 cgcaaagttg ctgaagacgc tgtaactcag gccgcgcctt tcgcgcacct gctcggtcag
      841 gcgtgagaca aaaccaccgc cgcccagaat gtagttgccc accgtcaggg cgaaatagtc
      901 cgggtcatcg cgcttgaagc cgggttggcc cagcaacacc tgggcctggg ccgagtcaaa
      961 cacaatgcgc tgctcgctgg cctgttccag cgcggccact tcagccacct ttggcaaggc
     1021 ggcacaacct gcctgcttgc tgcttgacag ccgcgcaaac agttcctgca ccagcgcatc
     1081 ggcctgcggc cgggtgacgg ccccgaccaa cgtgacgctg gcgcgacacg cttgcaccat
     1141 ctggcgatgc agggccgcca tgtcagccac ttcaaggcgc gacagagtgg cctcggtcat
     1201 ctcataaccg taggggtgat caccgtaaac cgctttggca aaggcccgag cggccagcgt
     1261 ggcagggcgg gtgttggcct ctttgaggct ggctttcagg ctgtcgcgct cgcgctgcca
     1321 gatgtcgggc ggaaaagacg gctcagccag ttgccgcgct gccagttgca cggctttggc
     1381 cagcaagtcg gggtaactca gggtgcgcag ggcaaagctg atgcggtcac tgctggcgct
     1441 ggcaccaaag ctggcaccca gatccgccca ggcctcgccc aaggcgtttt cgtccagcgc
     1501 aggctcttgc cgggccgggt tggcacgcac gcccttggcc agcatgcgcg ctgtcacgct
     1561 ggccagaccg gcctgctgtg gcgggtcgcg acggctgcca gcatcaaagt caatctgcac
     1621 atcaagcatc ggaatggccg ggcttggcac caggtacacc tgcaccccgc cggggctcgt
     1681 ccagtgctca atggcaatgc cagcctgcgc atggtttata agaaaaaggc ttgtagcgca
     1741 tatcaacagt gcgtagctag ctatagattt gatatttttc ataaggtttt tttgaaactg
     1801 gtcggtgccg caaggtggca cctgagaagt tggttagcgg gtgttggcaa agtttggcta
     1861 gcggctgggt cgggcagcag atcaacctga tcccgactga cttgcgcgca gatctgtact
     1921 gcccgtcagc gctgaatata taactgatat ggcgcaaagc gagcattctt cgcaatgctg
     1981 tgctgcaggg cgggggtacc ccttgtccag cagacgatca agagaggggt gaaaccgaat
     2041 tctccctatg tgatcgacaa tcagaggaaa cggaatgttg acttgaacga caatgctgcc
     2101 gtggtatccg tgattcgcga tgcactagca gggcaactgc tgtagtggcc ccatattcaa
     2161 ctgcggtaat aatagggaac ggactgcggt ttccagtttt ctagcaatgc tgaaattatg
     2221 aaacgcaaaa tctacgtcga aacgtccgtc atcagctacc tgacggctcg cccaagcaga
     2281 actattatcg gcgccgcaca tcaacaaatc acgcttgctt ggtgggaaac ccgtagccaa
     2341 tacgaactgt tggtgtcaga gtcggtatta cgcgagtgtg gcgctggcga tccagatgca
     2401 gctaaaaaac ggctggctgt gttgaatgat gtccccctgc ttttagtcac cgaacaggct
     2461 ctgaatattg ccgagtcatt ggtagaacgg gaaatcatcc cactgaaggc ggcagaagat
     2521 gcgttgcaca tcgcagtcgc cacggtcaat ggcgtggatt acctgctgac atggaactgt
     2581 cggcacatcg ctaacccgga aatacaaaga aacattgccc aataccttga acagattggt
     2641 ttgtttctgc catttatttg tacgccggaa gaacttcttg gagaagaaaa tgctgaatga
     2701 cgaaattatt gatgaagtgc gtgcaattcg cgaggcacat gccagcaagt ttggttatga
     2761 cttgcgtgca atttatgccg acctgaagaa gtccgaaggc aaacgtattg ccaatggcca
     2821 tccctacgtt tcccccccat ccgaaccgcc agcgcctaac tctgcattcc agcggattcg
     2881 cttcgctcac cgctgaattt gaacgttagg cgtcgcggtc aacgtgaatt gagtcaacag
     2941 gctgaaatag aaatagtggg ggcaacaaat gctgatgggt cgattgttga agccaagcga
     3001 agggcaaatt gtttatcact attgctcagc tgaaacactt cgagcaatat tagagtcaaa
     3061 gaaaatacgt tttacagaca taaatatgct caacgacgca tcagcat
//
LOCUS       DAOSLL010000584         3107 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_27289_length_3107_cov_4.183486, whole genome shotgun sequence.
ACCESSION   DAOSLL010000584 DAOSLL010000000
VERSION     DAOSLL010000584.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27289_length_3107_cov_4.183486"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="PLB25_19325"
     CDS             complement(<1..618)
                     /locus_tag="PLB25_19325"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HOE43757.1"
                     /translation="MGRFQLKRLLGQGAQAKVWLAFDPRLEREVAIKLMLPVQEANAT
                     AVNQWLQEARSVSRLTHPNIVPVFEADLQDQQPYLVFEYVAGKTLAQQLQADGAMSAT
                     QAGALLQDVLSALVAAHAAGVVHRDLKPSNVLIDSQGHARVMDFGIAARINLAQGHSA
                     AVEVAGSPAYMAPEAVRGEAISPLMDVYGAGLMFAEMLRGSPLRGG"
     gene            656..943
                     /locus_tag="PLB25_19330"
     CDS             656..943
                     /locus_tag="PLB25_19330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43758.1"
                     /translation="MSVEKGEKSTKVSSHFNAPLQVKKIEKTTPDVTSNAHLAINKRA
                     NRHHAAKFYKPSDDWATEWGEFVTGCQLAAEMLKTTRQAAQNSHCYKFEYA"
     gene            945..1676
                     /gene="ompR"
                     /locus_tag="PLB25_19335"
     CDS             945..1676
                     /gene="ompR"
                     /locus_tag="PLB25_19335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019428957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HOE43759.1"
                     /translation="MAAIAPRADKVLVVDDDTRIRDLLRRYLTQQGFEVLLAEDGKAM
                     NRTLQRETIDLIVLDLMMPGEDGLTLCRKLRSAKDLTPVIMLTAKGEDIDRIVGLEVG
                     ADDYLGKPFNPRELLARINAVLRRRPVAVHPGSPSSDGDVATFGQFYFDMGARILRKG
                     DEELPLTSGEFAMLKALVRHPRLPMSREKLAQLARGRGFEPFDRSLDVQVSRLRKLIE
                     ADAAVPRYIQTVWGVGYVFVPDGSD"
     gene            1663..3048
                     /locus_tag="PLB25_19340"
     CDS             1663..3048
                     /locus_tag="PLB25_19340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE43760.1"
                     /translation="MAAIETVDLDSEFGDAPDTPEHRPGLSLFWRTFFLQALLLLGCV
                     LAWAETLREMEFEPRALQTARQIASVVNLSRAAVMHSDAINRVSLFKTMKDQEQVTIS
                     LREPKDTFEPFGSDDISQHITEEIKSRLGPGSVVAKSVNGAEGLWVGFAIDSDHYWLK
                     TDRSRFDQPGGRTWMVWLLTAACLSLAGAAWIAGLINRPLKNLAIAANRVRAGNLEAS
                     KLDESVNTNEIRAVNVGFNRMTHKLAQIEHDRAVMLAGISHDLRTPLARLRLEAELSV
                     EDATARDNMANDIVQLDAIIGKFLDYARPGSIELRLISLNSAVEDTLTPLRKRSDVQF
                     RVALQDKLMVQADPVELQRVIGNLLENAVRYGKSVDHGVAEVDVSARQQGKKVLLRIR
                     DHGPGVPPEQVRQLTTPFFRGDSGRTSSNSTGLGLAIVEQTVARMGGAFQLSNARSGG
                     LCANLLLRSDG"
BASE COUNT          662 a          929 c          920 g          596 t
ORIGIN      
        1 gccaccgcgc agcgggctgc cgcgcagcat ctcggcgaac atcaggccgg caccataaac
       61 gtccatcagc ggcgaaatcg cttcaccgcg caccgcttct ggtgccatgt aggccgggct
      121 gccagcgact tccaccgccg cgctgtgccc ctgagccagg ttgatgcgcg cggcaatgcc
      181 aaagtccatc acccgggcgt gaccctggct gtcaatcagc acattggagg gtttcaggtc
      241 acggtgcacc accccggcgg cgtgtgccgc caccagcgca ctgaggacat cctgcagcag
      301 cgccccggcc tgggtcgccg acatggcacc atcagcctgc aactgctgcg ccagggtctt
      361 gcccgccaca tactcaaaaa ccaggtaagg ctgctggtcc tgcaggtcgg cttcgaacac
      421 cggcacgata ttcgggtgcg tcagccgact gacgctgcgc gcctcctgca gccactggtt
      481 gaccgccgtg gcgttggctt cttgcaccgg caacatcagc ttgatggcca cttcacgctc
      541 cagccgcgga tcaaaggcca gccagacctt ggcctgtgcg ccctgcccca atagccgctt
      601 gagctgaaaa cggcccaagg tggcaggctc ggccgcagcg ctcccagcca actgcttgag
      661 cgtcgaaaag ggtgaaaaat caaccaaggt aagttctcac ttcaatgcac cgttgcaggt
      721 caaaaaaata gaaaaaacaa cgccagacgt taccagcaat gcgcatctag ctataaacaa
      781 aagagcaaac aggcaccacg cagcaaaatt ctacaagccg tctgacgact gggctaccga
      841 gtggggcgaa tttgtaacag gatgtcaact tgctgcagaa atgctgaaaa caaccagaca
      901 ggccgcgcaa aatagccact gttacaaatt tgagtacgcg tagtatggcc gcaattgccc
      961 cccgagctga caaagttctt gtcgtcgatg acgacacacg catccgcgat ttgctgcgcc
     1021 gctacctgac gcagcaaggc tttgaggtgt tgctggcgga agacggcaaa gccatgaaca
     1081 ggacgctgca gcgcgaaacc atcgatctga tcgtgcttga cctgatgatg ccaggcgaag
     1141 atggcttgac cctgtgccga aagctgcgca gcgccaaaga cttgaccccg gtcatcatgc
     1201 tgaccgccaa gggcgaggac atcgaccgca ttgttggcct ggaagtcggt gccgacgatt
     1261 acctgggcaa gccgttcaac ccgcgcgaat tgctggcccg catcaatgcg gtgctgcgcc
     1321 gccgaccagt cgccgtgcat ccgggttcgc catcgtccga tggtgacgta gcaacatttg
     1381 gtcagttcta ctttgacatg ggggcacgca tcttgcgcaa gggcgatgaa gagctgcccc
     1441 tgaccagcgg tgaatttgcc atgctcaagg cgctggtgcg ccacccgcgc ctgccgatgt
     1501 cgcgcgagaa gctggcgcaa ctggcacgcg gtcgcggctt tgagcctttt gaccgcagcc
     1561 ttgatgtgca ggtatctcgc ttgcgcaagc tgattgaagc cgatgcagcg gtgccgcgct
     1621 acattcagac ggtctggggt gttggttacg tttttgttcc agatggcagc gattgaaact
     1681 gttgacctgg actcggaatt tggtgatgcg cctgatacac cggagcatcg acccggctta
     1741 agccttttct ggcgtacctt tttcctgcag gccttgctgc ttctcggctg tgtgctggcc
     1801 tgggccgaaa ccctgcgtga aatggaattt gaacccagag ccctgcaaac cgcccggcag
     1861 attgcctcgg tggtcaatct cagccgcgca gcagtgatgc actccgatgc catcaaccgg
     1921 gtgtcactgt tcaagaccat gaaagaccag gaacaggtca cgatctcact gcgtgaaccc
     1981 aaagatactt ttgagccatt tggcagcgat gacatcagtc agcacatcac cgaagagatc
     2041 aaatcgcggc tggggcctgg gtcggtagtg gccaaaagcg tcaacggtgc agagggcttg
     2101 tgggtcgggt ttgccatcga cagcgatcat tattggctga aaacagacag gtcccgcttt
     2161 gaccagccgg gtgggcgcac ctggatggtc tggctgctaa cggctgcctg cctatcattg
     2221 gcaggggccg cctggattgc cggcctgatc aaccgcccgc tgaaaaacct ggcgattgcg
     2281 gccaaccgcg ttcgcgccgg gaatttagag gccagcaagc tcgacgagtc cgtcaacacc
     2341 aacgaaattc gggctgtcaa tgtcggcttt aaccgcatga cgcacaaact ggcccagatc
     2401 gagcatgacc gagccgtcat gctggctggc atttcgcacg atctgcgcac cccgctggcg
     2461 cggctgcgac tggaagccga actcagtgtt gaagatgcca cagcacgcga caacatggcc
     2521 aacgacatcg tgcagctcga tgccatcatc ggcaagtttt tggactatgc ccgaccgggc
     2581 agcatcgaac tgcggttgat ttcgctcaac agcgcagttg aagacaccct gacgccgttg
     2641 cgcaagcgca gcgacgtgca gtttcgggtt gccttgcagg acaaactgat ggttcaagcc
     2701 gacccggttg agctgcagcg ggtaattggc aacctgctgg aaaacgccgt gcgttacggc
     2761 aagtcagtcg atcatggcgt ggcagaggtg gatgtttcag cgcgccagca gggtaaaaag
     2821 gtgctgctgc gcattcgcga tcatgggccg ggcgtgccgc ccgagcaggt caggcagctc
     2881 accaccccgt tttttcgcgg tgacagcggg cgcacctcct ccaacagcac cggtctgggg
     2941 ctggcgattg ttgagcaaac ggtggcacgc atgggtggcg cttttcagtt gagcaacgcc
     3001 cgcagtggcg gtttatgcgc caacctgctg cttcgctcag atggctaagg atatggtcgg
     3061 gaataactcc ccgttttgca ccaaggagtt gggatgcacg gctaggc
//
LOCUS       DAOSLL010000585         3083 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_27580_length_3083_cov_5.303831, whole genome shotgun sequence.
ACCESSION   DAOSLL010000585 DAOSLL010000000
VERSION     DAOSLL010000585.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27580_length_3083_cov_5.303831"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2179)
                     /gene="rnr"
                     /locus_tag="PLB25_19345"
     CDS             complement(<1..2179)
                     /gene="rnr"
                     /locus_tag="PLB25_19345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HOE43761.1"
                     /translation="MSLEEVEGIVQGHRDGHGFVIRDDGDADIYLPPNEMRAVLHKDH
                     VKVRIVRSDRKGRPEGRVVEIVERSCQVIIGRLLHESGIWIVAPEDKRYGQDIMIPKA
                     ATGSAKAGQVVVVELTEPPSLFGQPVGRIKEVLGEIDDPGMEIEIAVRKYSVPHEFSD
                     ACIALARALPDQVRAQDKRQRVDLTDVALVTIDGEDARDFDDAVYCEPAKIGKGKAAL
                     PGWRLLVAIADVSHYVENGSAIDVDAYDRATSVYFPRRVIPMLPEKLSNGLCSLNPEV
                     ERLCMVCDMLVSEDGDVSAYQFYPAVMWSHARFTYTEVAAILANTRGPEATKRKDRIG
                     NLINLHDVYLALLKSRVRRGAVDFETVETQIVCDDAGHIEKIVPRTRNDAHKLIEEAM
                     LAANVCSADFIAQSKHVGLYRVHEGPPPEKQAILRDYLKITGVGLTISDEPTPGEFQA
                     IANATKDRPDAAQIHSMLLRSMQQAIYTPINSGHFGLAFEAYTHFTSPIRRYPDLLVH
                     RIIKAILLKTRYALPTLPIPGEAHAKLSKRLEKNLASRVAEPGQKPRKLTAEMQAWQS
                     AGLHCSANERRADEASRDVEAWLKCKYMREHLGEEYAGVVSAVTSFGIFVTLDAMYVE
                     GLVHITELGGEYFRFDEARQELRGERTGLRYALGTRVRVQVSRVDLDGRRIDFRLVQE
                     GEALLLRPAKDKESSREPDERAPIEAESGSMSGQRRSRKDVQPL"
     gene            2227..2311
                     /locus_tag="PLB25_19350"
     tRNA            2227..2311
                     /locus_tag="PLB25_19350"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2261..2263,aa:Leu,seq:cag)
     gene            complement(2425..>3083)
                     /locus_tag="PLB25_19355"
     CDS             complement(2425..>3083)
                     /locus_tag="PLB25_19355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010460877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE43762.1"
                     /translation="AWVRLHYVYPYPNVDELLPLMAAGLILPYLDVPFQHSHPDVLRR
                     MKRPATGEKNLERIQRWRAACPDIVVRSTFIAGFPGETEEEFAHLLEFMREAKIDRAG
                     CFAYSPVHGAAANDLPGMLPEALREERRTRFMAVAEAVSATKLQQRVGATMQVIVDSA
                     PGLGKKGGVGRTYAEAPEIDGVVTLLPPEKISKTLKVGEFTKARIVGSHCHDLVAQPI
                     "
BASE COUNT          637 a          923 c          840 g          683 t
ORIGIN      
        1 ccagcggttg cacatctttt ctgctgcgcc gctggcctga catgctgccg gactcggcct
       61 caatcggcgc gcgctcatcg ggttcgcgcg aagactcctt gtctttggcc gggcgcaaca
      121 gcaatgcttc gccttcctgc accaagcgga agtcgatgcg gcgaccgtcg aggtcaaccc
      181 ggctaacctg cacgcgaacc cgtgttccca gggcgtagcg caagccagtg cgctcaccac
      241 gcagttcctg gcgcgcttcg tcaaaacgaa aatactcacc accaagttcg gtaatgtgca
      301 ccaatccctc cacatacatc gcatccaggg tcacaaagat gccaaagctg gtgaccgcgc
      361 tgacaacgcc ggcgtattct tcgccgagat gctcgcgcat gtacttgcac ttaagccagg
      421 cctccacgtc acggctggcc tcatcggcac ggcgctcgtt ggcactgcag tgcaagccgg
      481 ccgactgcca ggcctgcatt tccgcagtca gtttgcgcgg cttttgccct ggttcagcaa
      541 cgcgggaagc caggtttttc tccagccgct tggacagctt ggcatgcgct tcgcccggta
      601 tcggcaaagt tggcaaggca tatctggttt tcagcagaat cgccttgatg atccggtgca
      661 caagcaggtc ggggtagcgc cggatcgggc tggtgaaatg ggtgtaagcc tcaaatgcca
      721 gaccaaagtg gccgctgttg ataggggtgt aaatggcctg ctgcatcgag cgcaacagca
      781 tcgaatggat ttgagccgca tccggccggt ctttggtggc gttcgcaatc gcctggaact
      841 cacccggtgt cggttcatcg ctgatggtta ggccaacgcc ggtgatcttc aagtagtcac
      901 gcaggattgc ctgcttttcc ggtggcgggc cttcatgcac ccggtacaga ccgacgtgtt
      961 tgctttgcgc gatgaagtcg gcgctgcaga cattggcggc tagcatggct tcttcgatca
     1021 gcttgtgggc gtcgttgcgc gtgcgcggca caattttttc gatgtgtcca gcatcgtcgc
     1081 agacgatttg tgtctcgacc gtctcgaagt ccaccgcacc ccggcggacc cgggacttga
     1141 gcaatgccag atagacatcg tgcaggttga tcagattgcc aatgcgatct ttgcgtttgg
     1201 tagcctccgg gccgcgcgtg tttgcaagta tggcggccac ttcggtgtag gtaaagcgtg
     1261 catgactcca catcaccgca ggataaaact ggtaggccga tacgtcgcca tcttcagaaa
     1321 ccaacatgtc acagaccatg cacaggcgct ccacctcggg attcagcgag caaaggccgt
     1381 tggagagctt ctcgggcagc atcggaatga cacggcgcgg gaaatacacg ctggtggcac
     1441 ggtcataagc atcaacatcg atggccgagc cattttccac atagtggctc acgtctgcaa
     1501 tggccaccag caagcgccag ccaggcaagg ccgctttgcc ttttccaatt ttggctggct
     1561 cgcaatacac cgcgtcgtca aagtcgcgcg catcttcacc gtcgatggtg accagcgcca
     1621 catcggtcag gtcaaccctt tggcgcttgt cttgcgcccg cacctggtca ggcaaggcgc
     1681 gtgccagcgc aatacaggca tcagaaaact catgcggcac gctgtacttg cgcaccgcaa
     1741 tttctatttc catgccaggg tcgtcaattt cacccaggac ttctttgatg cgccccaccg
     1801 gctggccaaa cagcgatggc ggttcggtca attccaccac caccacctgc ccggccttgg
     1861 ccgacccagt ggcggccttc gggatcatga tgtcttgacc gtagcgcttg tcttcgggtg
     1921 caacgatcca gatgccgctc tcgtggagta aacgaccaat gatgacctgg cagctacgct
     1981 cgacaatttc gacgacccgt ccttcgggcc ggcctttgcg gtcactgcgc acaatccgca
     2041 ccttgacatg gtccttgtgc aaaacggccc gcatctcgtt ggggggtaga taaatgtctg
     2101 catcgccgtc atcacggatc acaaaaccgt gaccatcgcg atgcccttgg acaatgcctt
     2161 caacttcttc cagcgacaca ctggcttgct tgatattttt gatatactgc ctttctttcc
     2221 tgaaatgccc aggtggcgga attggtagac gcactagttt caggtactag cgagtaactt
     2281 cgtgggggtt cgagtccctt cctgggcacc aatattaatg aatttccggt tcgtttcaat
     2341 gtcagacacc ggtcgttttt gagcaatttg ccgctgccaa cacagcggct tttttgatgc
     2401 tggattcggt atcaacgaca gcattcaaat gggctgcgcc accagatcgt ggcagtggga
     2461 accaacaatg cgggccttgg taaattcccc gaccttcagc gttttgctga ttttttctgg
     2521 tggcagcaat gtcactaccc catcaatctc tggcgcctca gcgtaagtgc ggcccacgcc
     2581 acctttctta cctaaaccgg gtgctgagtc gacgataacc tgcatggtcg caccgacacg
     2641 ctgctgcaat ttagtggccg acactgcctc ggcaacggcc atgaacctag tccggcgctc
     2701 ttcacgcaag gcctcgggca gcatgcccgg caaatcatta gccgcagcac catggaccgg
     2761 gctataggca aaacaaccag cgcggtcaat tttggcttca cgcatgaatt ccagcaaatg
     2821 ggcaaactct tcttctgttt caccgggaaa tccggcaata aaagtgctgc gcaccacaat
     2881 atcagggcac gctgcacgcc aacgctggat ccgttccagg tttttttcgc ctgtggcagg
     2941 tcgcttcatg cgcctgagca cgtcgggatg actatgctga aaaggcacat cgaggtaagg
     3001 caagatcagt ccggcagcca tcaaaggcaa aagttcgtcc acattggggt aagggtagac
     3061 ataatgcaag cgcacccaag ccc
//
LOCUS       DAOSLL010000586         3079 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_27618_length_3079_cov_4.615741, whole genome shotgun sequence.
ACCESSION   DAOSLL010000586 DAOSLL010000000
VERSION     DAOSLL010000586.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3079
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27618_length_3079_cov_4.615741"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1902)
                     /locus_tag="PLB25_19360"
     CDS             complement(<1..1902)
                     /locus_tag="PLB25_19360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43763.1"
                     /translation="MIDSTEPQWPASAPVSASPEWAQTGLRQLLMYAGVGCVAGAVIA
                     LQIAIMRIFSEGSWAHFGSLVVSLAMLGFGLTSAIMCLARSWFERHWSGVIGGSLAAF
                     APLVVGANLVAQQLPFNPIFLVSDPLQKWHLVADFMLYLLPFLAGSLFLGTVFLRAKK
                     VFGRVYFADLLGSGLCGLLTLGSLFLLRPDNVVLVSVGLVGIGGLFWFAGLGSPKRAA
                     ILAGIAVASAAAHLYLPTALKIPTLAVSDYKGVSYAVKFPDSKRQYEGISPFGHLEVY
                     SSSYLHFAPGLSDNASLNLDEMPVDAYLGLYVDGDGPIGIMRDLSDKEAAYFHYLPMI
                     YPYLITDRPKTFVVQFGGGISTMVALHSNSRSVTVGESNPAFLKAFRESEALKTFTRN
                     ILDRVTVIPYDGRLFLANTSDRFDVIDLSLADSAGLSNPGGFAVVEKYAYTQEAMQHY
                     MQALTDAGILSVTLWNKEEPPKSVLKLYATMVGAARANGEKDIAQNFFVASSYLSTVT
                     VLYKRGGFTPAELVKLRQHTASMSFDEIYSPGIGVDAAKADHVFADFHDSIFSADTRQ
                     SPADASQAAESGLPEPANGNAITVPASLLSQLAWQSLIKDDWQGFSQRYLFDVRPLTN
                     NHPYFSAYVF"
     gene            complement(1899..2768)
                     /locus_tag="PLB25_19365"
     CDS             complement(1899..2768)
                     /locus_tag="PLB25_19365"
                     /inference="COORDINATES: protein motif:HMM:NF013315.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HOE43764.1"
                     /translation="MKNLPKKFACALIMVSAVFPFMANATVAKAFTYDMAPPTDSKDA
                     FVNWMVANRGEDKKFLGQRWDRYQALIQRKDLTNEQTKRSFLMTPREDFVRKISHVHS
                     YDDNFLDIGFGVTISGPHAVSRMTSVLDIQKNDKVLEIGTGSGYQSAILSRITNKVWT
                     IEIIKPLAQRTRGIYDSAIAKGYSEYKNINTKQADGYYGWEENAPFDKIIVTCGIDHI
                     PPALLKQMKTGGVMVIPVGPPGAQHVLKITKEANPDGSIKITRSDIYNGKVVPFVPFT
                     KLEGDAISGSHNK"
     gene            complement(2865..>3079)
                     /locus_tag="PLB25_19370"
     CDS             complement(2865..>3079)
                     /locus_tag="PLB25_19370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43765.1"
                     /translation="ASQLLGAKRGVIPDHILTPKITVIDNGSAIAKPMNEPVSLHIPG
                     LCHYRCPSFFSAAKWSPVRLPKVGAG"
BASE COUNT          728 a          813 c          874 g          664 t
ORIGIN      
        1 gaacacataa gccgaaaagt aggggtgatt gttggtcagg ggccgcacat caaacaggta
       61 gcgctggctg aacccttgcc aatcgtcctt tatcagcgac tgccaggcaa gttggctgag
      121 caaactggcg ggcacggtta tcgcgttgcc attggctggt tctggcaaac ctgactctgc
      181 tgcctggctg gcatcggctg gtgactggcg agtgtcagcg ctgaaaatgc tgtcatgaaa
      241 gtcagcaaag acatgatctg ccttcgcggc atcgacccca atgccggggg agtaaatttc
      301 gtcaaaggac atcgatgcag tgtgctggcg cagcttgacc agctcggctg gtgtaaagcc
      361 accgcgcttg taaagcaccg tgacggttga cagatagctc gatgcaacaa agaaattttg
      421 cgcaatgtct ttctcgccat tggcgcgtgc tgcgccgacc atggtggcgt agagtttgag
      481 cactgatttg ggcggctctt ccttgttcca caaggtcaca gacaagatac cggcatcggt
      541 cagggcctgc atgtagtgct gcatggcttc ttgcgtgtag gcgtattttt cgaccaccgc
      601 aaagccgccc ggattggaaa gtccggctga atcggccagg ctcaggtcga tcacatcaaa
      661 gcgatcactg gtgttggcaa gaaacaagcg accgtcgtaa ggaatgaccg taacgcggtc
      721 aagaatgttc ctggtgaatg ttttcaatgc ctcgctttcg cgaaatgcct tcagaaacgc
      781 agggttcgat tcgccaaccg tgacacttct ggagttgctg tgcagtgcca ccatggtgga
      841 aatgccaccg ccaaactgca cgacaaacgt cttgggccgg tcggtgatca gataggggta
      901 gatcatcggc aagtagtgaa agtaggcggc ttccttgtcc gacaggtcgc gcatgatgcc
      961 gatcgggccg tcgccatcga cgtacaagcc gaggtacgca tcaaccggca tttcgtccag
     1021 gttgagtgat gcattgtcag acaggcccgg tgcaaagtgc aggtatgagc tgctgtaaac
     1081 ctccaggtgg ccaaacggcg agatgccttc gtactggcgt ttgctgtccg ggaacttgac
     1141 cgcgtaagaa acgcccttgt aatctgaaac tgccagcgtg ggtatcttga gggctgttgg
     1201 caaataaagg tgtgcagcgg ccgaagcaac agcgataccg gccaaaattg ccgctcgttt
     1261 gggcgagccc aggccggcaa accagaaaag cccgccgatg cccaccagcc ccacagagac
     1321 cagcacaacg ttgtctggcc gcaacaaaaa caagctgccc agggtcagca aaccacacaa
     1381 gccggaaccc agcaagtcag cgaagtaaac gcgcccaaag acttttttgg cgcgcaggaa
     1441 caccgtcccc aaaaacaggc tgccggccaa aaatggcagc aggtacagca tgaagtcagc
     1501 caccaaatgc catttttgca gcgggtcaga gactaaaaaa atggggttga agggcaactg
     1561 ctgggcaacc aggttggcac caaccacaag cggcgcaaaa gccgccagtg agccaccaat
     1621 gacaccggac caatggcgtt caaaccaact gcgggccaga cacatgatgg cactggtcaa
     1681 accaaagcca agcatggcca gactgaccac cagcgaacca aaatgcgccc aactgccctc
     1741 cgagaagatc cgcatgatgg caatttgcaa cgcgatcacc gcgccggcga cacagcccac
     1801 gcccgcgtac atcagcagtt gacgcaagcc ggtttgcgcc cattcagggc tggcagaaac
     1861 aggggcgctg gccggccact ggggctcagt tgaatcgatc acttgttgtg tgaccccgag
     1921 atggcatcac cttccaattt ggtgaagggc acgaagggga cgaccttgcc gttgtagata
     1981 tccgagcgcg tgatcttgat gctgccgtca gggttggctt ctttggtgat tttgagcaca
     2041 tgctgtgcgc cgggcgggcc caccggaatc accatgacac caccagtttt catttgcttg
     2101 agcagtgccg gcggaatatg gtcgatgccg caggtgacga tgatcttgtc gaagggcgcg
     2161 ttttcttccc agccgtagta accgtcagcc tgcttggtat tgatgttctt gtactcgcta
     2221 taacctttcg cgatggcact gtcataaatg ccgcgtgtgc gttgcgccaa gggtttgatg
     2281 atttcgatgg tccagacctt gttggtaatg cgcgacagga tggccgactg atagcctgag
     2341 ccagtgccaa tttccagcac cttgtcgttt ttttggatat cgagcacgct ggtcatgcgg
     2401 ctaaccgcgt gcgggccaga aatggtgaca ccaaagccga tgtccaaaaa gttgtcgtca
     2461 taggagtgaa catgtgaaat cttgcgcacg aagtcttcac gcggtgtcat cagaaatgat
     2521 cgcttggttt gctcgttggt caggtctttg cgctgaatca gggcctgata acggtcccag
     2581 cgctggccaa ggaacttctt gtcttcaccg cgattggcga ccatccaatt gacaaaagcg
     2641 tccttgctat cggtaggtgg tgccatatca taggtaaatg ccttggcgac cgtggcgttt
     2701 gccatgaacg ggaaaactgc actgaccatg atcagtgcgc aagcgaactt tttgggcaaa
     2761 tttttcaaga gaattcctta ttgactgtgc tgaatccaaa ccaaagtgtc acaaagatgt
     2821 tggcaaatgt gccactcgcc agccgtttct ttgtccaatt cgcattatcc cgcgccaacc
     2881 tttggcaatc tgacaggtga ccactttgcc gcactgaaaa agcttgggca tcggtaatgg
     2941 cagagtcccg gaatatgcaa agagaccggt tcgttcatcg gttttgcgat ggcgctgccg
     3001 ttgtcgatca cggtgatttt tggcgtaagg atgtggtcgg gaataactcc ccgttttgca
     3061 ccaaggagtt gggatgcac
//
LOCUS       DAOSLL010000587         3073 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_27682_length_3073_cov_4.175944, whole genome shotgun sequence.
ACCESSION   DAOSLL010000587 DAOSLL010000000
VERSION     DAOSLL010000587.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3073)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3073
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27682_length_3073_cov_4.175944"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="PLB25_19375"
     CDS             complement(<1..541)
                     /locus_tag="PLB25_19375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HOE43766.1"
                     /translation="MSTLRILTGPVATVTLNRPEVRNAFNDEVIAELTQAFTQLGQDP
                     QVRAIVLAAEGPAFCAGADLNWMRRMADYTQAENLADAGQLAEMLRVIFTCPKPTVAR
                     IQGDVYAGGMGLVAACDMAVSVDSANYCLSEVKLGLYPATISPYVIRAMGARAAHRYF
                     LTAERFDALEALRIGFVHAA"
     gene            complement(571..2193)
                     /locus_tag="PLB25_19380"
     CDS             complement(571..2193)
                     /locus_tag="PLB25_19380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015015761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HOE43767.1"
                     /translation="MPVLETKLNARSADFQRNATAMRALVEDHNALVTQVEMGGGEAA
                     RAKHTARGKLLPRDRVQMLLDPGTPFLELSPLAAMGMYPDRDGSDSAPGAGMVVGIGR
                     VSGVDCMIVCNDATVKGGTYYPMTVKKHLRAQEVAQQNRLPCIYLVDSGGANLPNQDE
                     VFPDRDDFGRIFFNQATMSAMGIAQIAVVMGSCTAGGAYVPAMSDEAIIVKNQGTIFL
                     GGPPLVKAATGEVVTAEDLGGGDVHTRLSGVADHLAQNDMHALALARTAVKNLYQNKP
                     AAPADHAPVAPKYAASELYGVIPVDTRKPFDVREIIARVVDDSDFDEFKARFGATLIC
                     GFARIEGMPVGIIANNGILFSESALKGTHFIELCCQRKLPLVFLQNITGFMVGRKYEN
                     EGIARNGAKMVTAVATAAVPKFTIIIGGSFGAGNYGMCGRAFSPRFLWMWPNARISVM
                     GGEQAASVLATVRRDGIELKGGAWSLEEEEAFKAPVRRQYEEQGHPYFATARLWDDGI
                     IDPADTRRVLALGLAASRNAPIEDTKFGLFRM"
     gene            2329..>3073
                     /locus_tag="PLB25_19385"
     CDS             2329..>3073
                     /locus_tag="PLB25_19385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HOE43768.1"
                     /translation="MPRSARPVRPAPATGFVALTADAPSVTPIAFVKAITEAYARRGK
                     DPAGALNLAQIAPELLINPEARISAAQMERLSGAAMQELDDEGLGWFGRRLPWGSYGM
                     LARASISAPNLGVAMRRWCRHHGLIADDITLGLSVSGHTARLAISEQRDLQTLREFCL
                     VSVLRNFHGLACWLIDSRIVLNSAEFAFAPPAHQAVYQVLFSAPGRFNVAQTAIEFDA
                     RYLALPLRRDEAALQHMLKRALPLTVLQYR"
BASE COUNT          540 a          956 c          970 g          607 t
ORIGIN      
        1 ctgcggcatg cacaaagccg atgcgcagcg cctcaagtgc gtcaaagcgc tcggcggtca
       61 aaaagtagcg gtgtgccgcg cgtgcaccca tggcccggat cacatagggg ctgatggtgg
      121 cggggtagag gccgagcttg acttcgctta aacagtagtt ggcgctgtca acactcaccg
      181 ccatgtcgca cgcagccacc agccccatgc cgccggcgta cacgtcgccc tggatacgcg
      241 ccacggtggg cttggggcag gtgaagatga cacgcagcat ctcggccagt tgcccggcat
      301 cggccagatt ttctgcctgg gtgtagtcgg ccatgcggcg catccagttc aggtcggcac
      361 cagcgcaaaa ggctggccct tcagcggcca gcacgatggc gcgcacctgt gggtcttggc
      421 ccagttgggt aaaggcctgg gtgagttcgg caatcacctc gtcgttgaag gcgttgcgga
      481 cttcggggcg gttcagcgtg accgtggcga cagggccagt caaaatgcgc agtgtggaca
      541 tgttggtcgt ctttatcggg gttgcgggga tcacatcctg aacaagccaa atttggtgtc
      601 ttcaatcggc gcgttgcgac tggctgccag acctagcgcc agcacccggc gggtgtcggc
      661 ggggtcaatg atgccgtcgt cccacaaccg tgcggtggcg aaataggggt ggccctgctc
      721 ttcgtattgg cgacgcaccg gcgccttgaa ggcttcttct tcttccaggc tccaagcgcc
      781 gcccttgagt tcaatgccgt cgcggcgcac cgttgccaac acgcttgcgg cctgttcgcc
      841 gcccatcacc gaaatgcggg cgttaggcca catccacaaa aaacgcgggc taaaggcgcg
      901 cccgcacatg ccgtagttgc ccgcgccaaa gctgccgccg atgatgatgg tgaatttggg
      961 caccgctgcg gtggccaccg ccgtgaccat cttggcaccg ttgcgggcaa tgccttcgtt
     1021 ttcatatttg cgcccgacca tgaagccggt gatgttttgc aagaacacca gtggcagctt
     1081 gcgctggcag cacagttcaa tgaaatgcgt gcccttgagg gctgactcgg aaaacaaaat
     1141 gccgttgttg gcgatgatgc cgaccggcat gccctcgatc cgggcaaagc cgcagatcag
     1201 cgtggcacca aagcgcgcct tgaactcgtc aaagtccgag tcatcgacga cgcgggcgat
     1261 gatttcacgc acgtcaaagg gtttgcgggt gtccaccggg atcaccccat aaagctcgga
     1321 tgctgcatat ttaggagcta ccggcgcatg atctgcgggg gctgcaggct tgttttgata
     1381 caaatttttg accgccgtgc gggccagcgc cagcgcgtgc atgtcgtttt gcgccaagtg
     1441 gtcggcgaca cctgagagcc gcgtgtggac atcgccaccg cccaggtctt cggctgtgac
     1501 cacctcaccg gtggcggctt tcaccagcgg cgggccaccc agaaagatcg tgccctggtt
     1561 tttgacgatg atggcctcgt cgctcatcgc tggcacgtag gcaccaccgg cggtgcaact
     1621 gcccatcacc accgcaattt gcgcgatgcc catggcactc atggtggcct ggttgaaaaa
     1681 gatgcggcca aagtcgtcgc ggtcggggaa cacctcgtcc tgattgggca ggttggcccc
     1741 gcccgagtcc accaggtaaa tgcagggcag gcggttttgc tgcgccacct cttgcgcccg
     1801 caggtgtttt ttgaccgtca tcgggtaata ggtgccgcct ttgacggtgg catcgttgca
     1861 gacaatcatg cagtccaccc cgctaacccg cccaatgccg accaccatgc cggcaccggg
     1921 cgcgctgtca gagccgtcgc ggtcggggta catgcccatt gccgccagtg gcgagagttc
     1981 cagaaacggg gtgccggggt cgagcagcat ttgcacccgg tcgcgcggca gcagcttgcc
     2041 acgcgccgtg tgtttggccc gcgccgcctc gccgccaccc atttccactt gcgtgaccaa
     2101 ggcgttgtgg tcttccacca gggcgcgcat ggcggtcgcg ttgcgttgaa aatcggcaga
     2161 gcgggcgttg agtttggttt ccagaacggg catggcattc tttctgtgtt tcaaattggg
     2221 gcggcaaacc ggcaagcgct gtcgctgcgc ccaagcatag aacagcaggc atgcgcctga
     2281 ctgccatgca ggttgcaaat gctgccaggt ggcgtgatac tgccaggcat gccccgatca
     2341 gcccgcccag ttcgcccagc acccgccacc ggcttcgttg ccttgacggc tgatgcgcca
     2401 tcggttaccc cgattgcctt tgtcaaggcg atcacagagg cctatgccag gcgcggcaaa
     2461 gacccggccg gggcgctcaa tctggcacaa attgcgccag aactcctgat taaccctgaa
     2521 gcccgcatca gcgccgcaca gatggagcgc ctgtcgggtg ccgcgatgca ggaactcgac
     2581 gacgagggcc tgggctggtt tggccgacgc ctgccctggg gcagttacgg catgctggcg
     2641 cgggcctcca tcagcgcgcc gaatctgggc gtggcgatgc ggcgctggtg ccgccaccat
     2701 ggtttgattg ccgacgacat cacgctgggc ctgtcagtca gcggccacac cgcccggctg
     2761 gcgatctcgg agcagcgcga cctgcagacg ctgcgcgagt tctgcctggt atcggtgctg
     2821 cgcaactttc atggcctggc gtgttggctg attgactcac gcattgttct gaacagcgcc
     2881 gagtttgctt ttgcgccgcc cgcccatcaa gctgtctatc aagtgctttt ttcggctcca
     2941 gggcgcttca atgttgcgca gacagctatt gaatttgatg cgcgttacct ggccttgccg
     3001 ctgcgccggg acgaggctgc gctgcagcac atgctcaaac gcgcgctgcc cctgacggtg
     3061 ttgcaatacc ggc
//
LOCUS       DAOSLL010000588         3069 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_27715_length_3069_cov_4.321500, whole genome shotgun sequence.
ACCESSION   DAOSLL010000588 DAOSLL010000000
VERSION     DAOSLL010000588.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3069)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3069
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27715_length_3069_cov_4.321500"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1992)
                     /locus_tag="PLB25_19390"
     CDS             complement(<1..1992)
                     /locus_tag="PLB25_19390"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015012784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HOE43769.1"
                     /translation="MSESPAPVPKKPTPSAAIGLGRAGANAAAASFASLTSKLDYLSP
                     ADLTNVRLAYRFADQAHLGQLRNSGEPYITHPIAVAAQCAEWKLDAQALMAALLHDAM
                     EDCGITKPDLIERFGSPVAELVDGLTKLEKLHFNTREENQAESFRKMLLAMARDVRVI
                     LIKLADRTHNMRTLPNAPREKWSRIASETLDIYAPIAHRLGLNQTYRELQDLSLRYLR
                     PWRYGVLSKAVVKARGRRRDLIHKVQADLESVFSGHGLTVKIAGREKTLYSIYKKMTE
                     KNLSFAQVTDIYGFRVIVPHLIDCYTALGLLHQLYKPVPGKFKDHIAISKINGYQSLH
                     TTLVGPSGVNVEFQVRTEAMHLVAETGVAAHWLYKEVDQGDLSRSDRMGSKWLQSLLD
                     IQDETRDAAEFWDHVKVDLFPDAVYVFTPKSQIMAMPRGATVVDFAYAIHSNVGDHAI
                     AARINNEEVPLRTEVKNGDVIEIISDAESMPNPAWLGFVRTARARSKIRHHLKTLAQT
                     ESESLGEKMLAQALRADGIENLPDETAANLVMWDKLLRFSGNKTRSELLTDIGLGKRI
                     ASIVAKRLTKLLTDNGEKPDALLITRERFAAGENVAQGGVLIDGSESTSVRFASCCRP
                     IPGDSIVGYLGRGEGLTIHSADCLVAKKLQYKDSERFIGV"
     gene            complement(2087..2290)
                     /gene="rpoZ"
                     /locus_tag="PLB25_19395"
     CDS             complement(2087..2290)
                     /gene="rpoZ"
                     /locus_tag="PLB25_19395"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HOE43770.1"
                     /translation="MARITVEDCLLLIPNRFQLVLAASYRARMLSHGHTPKVDSKNKP
                     GVTALREIAAGKIGIEMLKKVPL"
     gene            complement(2325..2945)
                     /gene="gmk"
                     /locus_tag="PLB25_19400"
     CDS             complement(2325..2945)
                     /gene="gmk"
                     /locus_tag="PLB25_19400"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008905074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HOE43771.1"
                     /translation="MEYPGNLFVVAAPSGAGKSSLVKALLELDSNLQLSVSHTTRAPR
                     GQEKHGREYFFASEPEFDAMVRADAFVEWAHVHNHRYGTSKKAIEERMAQGADVILEI
                     DFQGALQIKKAFANAVTIFILPPSWDELRSRLERRGEDTPQVIDTRLQNAAQEMAQVH
                     QFDFVIINELFDRALFDLKTVVHSQRLKYLAQRRARSETFKALKIS"
     gene            complement(2984..>3069)
                     /locus_tag="PLB25_19405"
     CDS             complement(2984..>3069)
                     /locus_tag="PLB25_19405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HOE43772.1"
                     /translation="AALELTRISVDMKVLIEQMREQVQNIE"
BASE COUNT          643 a          857 c          868 g          701 t
ORIGIN      
        1 aaccccgatg aaacgctcgc tgtccttgta ctgcagcttt ttggcaacca ggcagtcagc
       61 agagtgaatg gtcaggcctt caccacggcc gaggtaaccg acgatgctgt cgccgggtat
      121 tggcctgcaa caactggcaa aacgcactga ggtgctttcg ctgccgtcga tcaaaacccc
      181 accctgggcg acattttcgc cagccgcaaa gcgctctcgg gtaatcagca aggcatcagg
      241 tttttcgccg ttgtcagtca acagcttggt gagccgtttg gccacaatgc tggcaatgcg
      301 cttgcccaag ccgatatcgg tcagcagttc agagcgcgtc ttgttgccgc tgaagcgcaa
      361 caacttgtcc cacatgacca ggttggcggc ggtctcgtcg ggcaagtttt caatgccgtc
      421 ggcgcgcagc gcttgtgcca gcattttttc gcccaggctt tctgactcgg tttgcgccag
      481 ggtcttgagg tggtgacgaa tcttggaacg cgcgcgtgca gttcgcacaa aacccagcca
      541 ggctggattg ggcatggact cggcatcgct gatgatctcg atcacgtcac cattcttgac
      601 ttcagtgcgc aatggcactt cttcattgtt gattctggcc gcaatcgcgt ggtctccgac
      661 attgctgtga atggcgtagg caaaatccac cacggtggca ccgcgtggca tcgccatgat
      721 ctggctcttt ggggtgaaca catagactgc atcaggaaac aggtccactt tgacatggtc
      781 ccaaaactcg gcagcgtcgc gggtctcatc ctggatatcg agcagtgact gcagccattt
      841 ggagcccatc cggtcactgc ggcttaagtc gccctgatca acttccttgt acagccagtg
      901 cgcggccacg ccggtttcgg ccaccaggtg catggcctcg gtgcgtacct gaaactccac
      961 gttgacaccg gacgggccaa ccaaagtggt gtgcagcgac tggtagccat tgatcttgct
     1021 gatggcaata tggtccttga acttgccggg taccggtttg tagagctgat gcagcagccc
     1081 cagcgccgtg tagcagtcaa tcaggtgcgg cacgatgacg cgaaacccgt agatgtccgt
     1141 tacctgggcg aagctcaggt ttttctcggt catcttcttg taaatggaat agagggtttt
     1201 ttcacgcccg gcgatcttca cggtcagacc gtggccggaa aacacggatt ccagatctgc
     1261 ctgaaccttg tgaatcaggt cgcgacgcct gccgcgcgcc ttgaccacgg ctttcgatag
     1321 caccccatag cgccagggtc gcaggtatcg cagcgacaag tcctgcaatt cgcgataggt
     1381 ctgattcaag cccaggcggt gcgcaatcgg ggcataaatg tcaagtgttt cagacgcaat
     1441 gcgcgaccac ttttcgcgtg gcgcgttagg cagggtgcgc atgttgtggg tgcggtcggc
     1501 cagcttgatc aggatcaccc gcacatcacg tgccatggcc agtagcatct tgcgaaacga
     1561 ttcagcctga ttttcctcgc gtgtattgaa gtgcagtttc tccagcttgg tcaggccgtc
     1621 aaccagttcg gccaccggtg agccaaagcg ctcaatcagg tctggtttgg tgatgccgca
     1681 gtcttccatc gcatcgtgca gcagcgccgc catcagcgcc tgggcatcaa gcttccattc
     1741 ggcacactgg gctgctaccg caatcggatg ggtgatgtag ggctcgccac tgttcctgag
     1801 ctgacccagg tgtgcctgat cggcaaaacg gtaggccaga cgaacattgg tgaggtcagc
     1861 cgggctgagg taatcgagtt tgctggtcag actggcaaaa ctggcggcag ccgcattggc
     1921 acccgcccgg ccaagtccaa tggcggccga cggtgtcggc tttttgggta cgggcgcggg
     1981 tgactcactc atgactcaaa tatagcgtac caggcactgc gcccagagct caaataacaa
     2041 aagcacccca aagggtgctt ttgacgggaa atgtggacta agtcgcctac agcggcacct
     2101 ttttcagcat ttcaatgcca atttttccgg ctgcaatttc gcgcagcgca gtcacgcctg
     2161 gcttgttctt gctgtcaacc ttgggagtgt gcccgtggct gagcatgcgc gcccggtatg
     2221 acgctgccaa caccaactga aaacggttgg gtataagcaa caggcagtct tcaacggtaa
     2281 tacgggccat gaaatctcca aaactaaacg ctgggtaagg gacactaaga aattttcagt
     2341 gccttgaatg tttcagatct tgcgcggcgc tgtgcaagat acttgagacg ctgtgagtgc
     2401 acgactgttt tgaggtcaaa aagtgcgcgg tcaaacaact cgttgattat aacgaagtca
     2461 aactggtgca cctgtgccat ctcttgggcg gcattttgca gccgggtatc aatcacctgt
     2521 ggcgtgtctt cgccacgacg ctccagcctg gaacgaagct catcccaact gggcggcaaa
     2581 atgaaaatgg tgaccgcatt ggcaaaagct tttttgatct gcaaggcccc ctgaaagtcg
     2641 atttcgagaa tcacatcagc gccctgcgcc atgcgctctt caatggcttt tttggaggtt
     2701 ccgtagcggt ggttgtgcac atgggcccat tcgacaaaag catcggcccg gaccattgca
     2761 tcgaactcgg gttcagaagc aaaaaaatac tcgcggccgt gcttttcctg cccgcgcggt
     2821 gcgcgcgtgg tatgcgaaac cgacagttgc aaatttgagt caagctccag cagcgccttg
     2881 accaagctgg atttgccagc gccactgggt gcagccacca caaacaggtt gcccgggtac
     2941 tccatcgtgt tgtctctggt aaaaaggtgg ggttacagcc ggtttattct atgttttgca
     3001 cttgttcgcg catctgctcg atcagcactt tcatgtccac cgagatgcgg gtcagttcca
     3061 gcgcggctg
//
LOCUS       DAOSLL010000589         3068 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_27727_length_3068_cov_3.444408, whole genome shotgun sequence.
ACCESSION   DAOSLL010000589 DAOSLL010000000
VERSION     DAOSLL010000589.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3068
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27727_length_3068_cov_3.444408"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            363..1184
                     /locus_tag="PLB25_19410"
     CDS             363..1184
                     /locus_tag="PLB25_19410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HOE43773.1"
                     /translation="MTTPSISMIVTVRASSEARRHNLSYVLDWYQQMPDWELLVVEQD
                     QAPELDSSEWRYTTKRLYVVNPGPFNKGWGFNVGARAARSDVLFFCDADLLLAHSALK
                     AAQSLCSRRALAVKPFDSLIDLNQTDTQAILSGRLAPDFEREDASKFRGDREALCFCG
                     GAFFMRRNLYNALGGFDERYLGWGAEDDAITVKLQRMTTELAVLEGRAAVHLWHERGD
                     LATFGNPHYPNNLRMLQEVAQASADSLRFQSDLQRQIMGNPGKYQPAVPEKSASL"
     gene            1522..2517
                     /locus_tag="PLB25_19415"
     CDS             1522..2517
                     /locus_tag="PLB25_19415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bug family tripartite tricarboxylate transporter
                     substrate binding protein"
                     /protein_id="HOE43774.1"
                     /translation="MTTIKTLRFRKFLQTLGLVLALGAMSATGAARAQSSAIRLMVGF
                     PPGGGTDAIARILADKLKDQLGVAVVVDNKAGAGGQIAAQALKAAAADGNTLFLSHDH
                     TISILPLVVKNPGFDPARDFVAVAGFATFVNALALSGGTPARSLAEYLAWVQQQGAGK
                     GTVGVPAPASVPEFLVGVIGRKYKLDLQAAPYRGSAPMMADMLGNQINAGVGSVPDFI
                     ENQKAGKIRVVAVLGNARQAAMPDVPTFAELGLAGFEDVPYYGIFAPRGTPPATIDKL
                     SAAVAKVVALPDVHERLSHLGLTVGYMTPTQLASREQAYARTWARIIQDSGFKSQ"
     gene            complement(2823..>3068)
                     /locus_tag="PLB25_19420"
     CDS             complement(2823..>3068)
                     /locus_tag="PLB25_19420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HOE43775.1"
                     /translation="TPSWPLAGALLFIALGPAVLAYRAWGAGVSRAGPSVAGFFANLT
                     PLFTAVLSSLFLGELPRLYHALAFALIVGGIVVSSRR"
BASE COUNT          622 a          876 c          924 g          646 t
ORIGIN      
        1 agtggctttg gcctgtgact gggccatttc accccatgcc ttcaggttgt ccatgacggg
       61 cttcatggct tcctgggcgg cagccgggtt aaatttgcca gcgctgtctt gcatggtttt
      121 ggcaatggct tcgaagggag tgttttcaaa catttgaggt ccttggttaa aattgttgca
      181 gtgcagcaat tatcaggcaa aaaaatccgg atgggaagat ttattgttat ttttgttttt
      241 tttaggggca ttgccccaga aagtgtcttt gccaggatgt ggcgctggcg ctgggcgaat
      301 aaactgcggc acctgtccag tgacggttgc atcaatcggc ctggaggcaa ccgagccaca
      361 ccatgacaac acccagcatt tccatgatcg tgacggtccg cgcttccagc gaggccaggc
      421 ggcacaacct gtcttatgtc ctggactggt accagcaaat gccggactgg gaactgctgg
      481 tggtggagca ggatcaagcc cccgagcttg acagctctga atggcgctac accaccaaac
      541 ggctgtacgt tgtcaaccct gggccgttca acaagggttg gggctttaac gtgggggcca
      601 gggccgcgcg aagcgatgtg ttgttttttt gcgatgcgga cctgctgctg gcgcacagcg
      661 cgctgaaggc agcacaaagc ctgtgcagtc ggcgcgcatt ggcggtcaaa ccctttgata
      721 gtttgatcga cctgaaccag actgatacgc aggccatcct gtctgggcga ttggcaccgg
      781 actttgaacg tgaagatgcc agcaaattcc ggggtgatcg tgaagcgctg tgtttttgtg
      841 gcggggcttt tttcatgcgc cgcaatcttt acaacgcact gggcggcttt gacgagcgct
      901 accttggctg gggtgctgaa gacgatgcca tcacagtcaa attgcagcgc atgacgacgg
      961 agctggcggt gctggaaggc cgcgctgcgg tgcacctctg gcatgagcgt ggcgatttgg
     1021 ccacattcgg caatccgcac taccccaaca atctgcgcat gctgcaggaa gtggcccagg
     1081 ctagcgccga ttcgctgcgt tttcaatccg atctgcagcg ccagatcatg ggcaacccag
     1141 ggaaatatca acctgcagtg cctgaaaagt cggcaagcct gtgatgcttg tcacaaggtc
     1201 aaattcaccg gcttcccaac gggccggttc agcgctgagg cttttgcagc caggtggtgc
     1261 gtttgaccac aacgctgaca tgaaatggcg tcatttcccg cctggctttg ttgttcgtct
     1321 tggcaccagc cacggccaag tcgcctgctc gtgccaggcc agcctgacaa atcccatcga
     1381 tttcatttcg tgatcgtttt gatgaagcaa actgctgtcg atgagcgtga aaacctgtgt
     1441 ttccacgctg gccaggttgg catcgaccgg tctgctgcgt cttgtccttg ccttgcaatc
     1501 actttttctt gacccctcac catgaccacc atcaaaaccc ttcgtttcag aaagttcctt
     1561 cagacactcg gcctggtgct tgccctgggt gccatgtctg cgaccggcgc ggcccgggct
     1621 caaagcagtg ccatcaggct gatggtgggt ttcccgcctg gcggcggtac cgatgccatt
     1681 gcacgcatcc tggcagacaa gctcaaagac cagttggggg tggcggtggt ggtggacaac
     1741 aaggctggtg ccggtggcca gattgcggct caggcgctta aagcggccgc tgcggacggc
     1801 aacacgctgt ttttgtcgca cgaccatacc atctcgatct tgccgctggt ggtgaaaaac
     1861 cccggtttcg atccagccag ggactttgtt gcagtggctg gctttgccac ctttgtcaat
     1921 gcgctggcgc tgtcaggcgg caccccggcc aggtcgctgg ccgagtatct ggcctgggtg
     1981 caacagcagg gcgctggcaa aggcacggtg ggtgtgcccg cacccgcatc ggtgccggag
     2041 tttctggtgg gggtgatcgg tcgcaaatac aaacttgatc tacaggccgc cccgtaccgc
     2101 ggcagcgccc cgatgatggc ggacatgctg ggcaaccaga tcaatgccgg ggtcggctcg
     2161 gtgcctgact tcatcgaaaa ccagaaggcc ggcaagatcc gggtggtggc cgtgctgggc
     2221 aacgcccgcc aggctgccat gccggatgtg ccaacgtttg ccgaactggg cctggctggt
     2281 tttgaggatg tgccctatta cggcatcttt gcaccccgag gcacgccacc tgcaaccatc
     2341 gacaagctct ctgccgcggt tgccaaagtg gttgcgctgc ccgatgttca tgagcgcctg
     2401 agccacctgg gactgactgt gggctacatg acaccgaccc agttggccag tcgcgaacag
     2461 gcctatgcca gaacctgggc gcgcatcatt caagacagcg gtttcaagtc gcagtagctg
     2521 gctggtttgt cggcgacttt atacaaagaa acaggctgta gcccccgtca gcaaagcgca
     2581 agcagctata taaatagtag taccgtaagc ccggatcaga ccacctgaca gcgcaggttt
     2641 cgaccgctga cccaggggct tttaggatgt ggtcgggaat aacaccctgt ttgcaccaag
     2701 gagttgggat gcacggctag gcgcgagacg cgccgtttag ctggctaaac aagcgtctcg
     2761 caacgacgcc gtgcgcccaa atccgccggt gcaaataggg gaagttactt ctgaccacat
     2821 ccttagcgcc gcgacgacac cacaatgccg cccacaatca gcgcaaaggc cagtgcgtga
     2881 tacaggcgcg gtagttcacc cagaaacagg cttgacagca cagccgtgaa caacggggtc
     2941 aggttggcaa aaaagccggc caccgaggga cccgcgcgag acacaccggc accccaggca
     3001 cgataggcca gcactgccgg gcccagtgca atgaacagca gcgcgcccgc cagcggccag
     3061 cttggggt
//
LOCUS       DAOSLL010000590         3054 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_27912_length_3054_cov_4.106702, whole genome shotgun sequence.
ACCESSION   DAOSLL010000590 DAOSLL010000000
VERSION     DAOSLL010000590.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27912_length_3054_cov_4.106702"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..546
                     /locus_tag="PLB25_19425"
     CDS             121..546
                     /locus_tag="PLB25_19425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOE43776.1"
                     /translation="MTSLSTLALSRRQLSIFVLGLGLSLSACSQTATSADSVTLEFAR
                     TELEAGRAILIDVREPDEHATGVAKGAQLLPMRQLGARLAEIPSASDKPVLLICNTQN
                     RSSATLSALRERGYKNLRFVQGGMSEWNRRGWPVVKPKA"
     gene            complement(568..1500)
                     /locus_tag="PLB25_19430"
     CDS             complement(568..1500)
                     /locus_tag="PLB25_19430"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019577611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HOE43777.1"
                     /translation="MNPSNLHPMVNVAIKAARAAGAIINRAALDVESVRVSQKQVNDF
                     VTEVDQAAEQVIIETLLGAYPGHGIWAEESGHEHGAKDSECVWIIDPLDGTTNFIHGL
                     PIYSVSIALAVRGKIEQAVIYDPSRNDLYTATKGRGAYMNNRRLRVAKRTRLQECLIA
                     TGFPYRFDDDINTYLRMLGDIMQRTAGLRRPGSAALDLANVAAGFTDGFFELGLQPWD
                     VAAGSLLITEAGGLVGNLSGDANFLEQHECLAANPRIYGQLVAVLGKYSKFASAGEKA
                     TVRAAVAELALPKSPTGPALSESDDSSTQPDAPF"
     gene            1610..2500
                     /locus_tag="PLB25_19435"
     CDS             1610..2500
                     /locus_tag="PLB25_19435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010459926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HOE43778.1"
                     /translation="MQTRFILINTSHAGNVGATARAMKTMGFSDLVLVAPRWPNVLNR
                     EETIQRASGALDVLKNARIVATLDEALQGMTHLCATAMTPRDFGPPTMTPRGYFESLL
                     KKELPALLVQGLEAKNPDEFSRPPGAPDMLHNKELSAQADQGPEAKKAGHFGLALLFG
                     SERFGMRNEDVYRCHVCLSIPSNPQFGSLNLASALQVIAYDWRVALGAYPVHGASASP
                     ALADASQVAGMLAHLEQALTAIGFLDPQAPKKLMPRLNQLFNRAGVTEEEIHILRGMA
                     KMMLKPQPQMATEFGRKDET"
     gene            2543..>3054
                     /locus_tag="PLB25_19440"
     CDS             2543..>3054
                     /locus_tag="PLB25_19440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43779.1"
                     /translation="MIIYFFSFDVAHPLGDSATHPIEPQASRLGRKLLLAGSGCASSS
                     PLQGATMPHAWPAQAVQPCAGRFGAVAFALLRRAANGPLGRVQLRNLGAMRIKLTNML
                     SVFADCLGQQAPRFEPVVGHGCSPKANLIFSSLPGKMPGLKQVKSLQIMRRTQTFKVI
                     RREGATPTNS"
BASE COUNT          607 a          938 c          905 g          604 t
ORIGIN      
        1 gtgcatccca actccttggt gcaaaacggg gagttattcc cgaccacatc cttatatgat
       61 caagccctgg tctcttcacg gactctctgc gacacaaaac cgcttcaact ttttttcacc
      121 atgacatcac tttccaccct ggcgctgtcg cgccgccaat tgagcatttt tgttttgggc
      181 ctgggcttgt cgctgtcggc ctgcagccag acggctacca gcgctgattc ggtcacgctc
      241 gaatttgcgc ggaccgaact cgaagccggg cgcgccatat tgatcgatgt ccgcgaaccc
      301 gatgagcacg ccactggtgt ggccaaaggc gcgcaactgc tgccgatgcg ccagttgggt
      361 gcccggctgg ccgagattcc gagcgcctcc gacaagccgg tgctgttgat ctgcaacacc
      421 cagaaccggt caagcgccac cctgagtgcg ttgcgcgagc ggggctacaa gaacctgcgt
      481 tttgtccagg gcggcatgag cgaatggaac cggcgtggct ggccggtggt gaaaccaaag
      541 gcctgacaca gcgcccgcgc ggccagccta aaacggcgcg tcgggctgcg tgctgctgtc
      601 gtctgactca gacagcgctg gcccggtcgg cgacttgggc agtgccaact ctgccactgc
      661 ggcccgcact gtggcttttt cgccagcact ggcaaacttg ctgtatttgc ccagcacggc
      721 aaccaactgg ccgtaaatgc gcggattggc tgccaggcac tcgtgctgct ccaaaaagtt
      781 ggcatcgccc gacaggttgc cgaccagccc gccagcctcg gtgatcagca gcgaacccgc
      841 cgccacatcc cagggctgca gcccaagttc aaaaaaacca tcggtaaagc cggccgccac
      901 attggccagg tcaagcgctg ccgagcccgg gcggcgcaac ccggcagtgc gctgcatgat
      961 gtcgccgagc atgcgcaggt aggtgttgat gtcgtcatca aagcggtacg gaaaaccggt
     1021 ggcaatcagg cattcctgca gccgggtgcg cttggccacc cgcaggcgtc ggttgttcat
     1081 gtaggcaccg cggcccttgg tggcggtata caggtcgttg cggctggggt cgtaaatgac
     1141 ggcttgctcg atcttgccgc gcaccgccag cgcaatgctg acgctgtaaa tcggcaggcc
     1201 gtggatgaaa ttggtggtgc cgtccagcgg gtcgatgatc cagacacatt cggagtcttt
     1261 ggccccatgc tcgtgaccag actcttcggc ccagatgcca tggccggggt aagccccgag
     1321 cagggtctcg atgatgacct gctcggcggc ctgatccact tcggtgacaa agtcgttgac
     1381 ctgcttttgc gagacacgca ccgattccac gtcaagtgcg gcgcggttga tgatggcacc
     1441 ggcagcgcgc gcggccttga tggccacgtt gaccatggga tgtaagttgg atgggttcat
     1501 gggtgaggtt agggttggcc aggcagcaag cgggtctgtt ctgtgcgaaa agcgggcacc
     1561 aggcggcaat cggtgggcgg cgacaatagc ccgattttag cggcccacca tgcagacccg
     1621 attcatcctg atcaacacca gccatgccgg caatgtcggg gcgacggcgc gcgccatgaa
     1681 aaccatgggc ttttctgacc tggtgctggt ggccccgcgc tggccaaatg tgctcaaccg
     1741 tgaagaaacc atccagcgcg ccagcggtgc gctcgacgtg ctgaaaaatg cccgcatcgt
     1801 cgccacgctc gacgaagcgc tgcaaggcat gacccacctg tgcgccaccg ccatgacccc
     1861 gcgagacttc ggaccgccaa caatgacccc gcgcgggtat ttcgaatcgc tattaaaaaa
     1921 agagctgcca gcactactgg tacaagggct ggaggccaaa aaccctgatg aattcagcag
     1981 accgcctgga gcccccgata tgctacacaa taaagagcta tcagcgcagg cagatcaagg
     2041 gccagaggcc aaaaaggctg gtcatttcgg gttggccctg ctgtttggct cggagcgttt
     2101 tggcatgcgc aacgaggacg tttaccgctg ccatgtgtgt ttgagcattc ccagcaaccc
     2161 gcagtttggc tcgctcaacc tggcctctgc gctgcaggtg attgcttacg attggcgggt
     2221 ggcgctgggt gcctatccgg tgcacggcgc cagcgccagc ccggcgctgg ccgatgccag
     2281 tcaggtggcg ggcatgctgg cgcatctgga gcaggcactg actgccattg gctttctgga
     2341 cccgcaggcc cccaaaaagc tgatgccccg cctgaaccag ttgttcaacc gcgctggcgt
     2401 aaccgaagaa gaaatccaca ttctgcgcgg tatggccaag atgatgctca agccgcagcc
     2461 ccagatggcg accgaatttg gccgcaaaga cgagacttaa acctagattc agttgaccgc
     2521 ttgttttcct ctataactcc ttatgatcat ttactttttt agcttcgatg tggctcaccc
     2581 gttgggtgac agcgctacgc acccgattga accgcaggca tcgcgcctgg ggcgcaagct
     2641 cctcctagcg ggcagtggct gtgcgtcgtc gtcgcccctg cagggcgcta ccatgccgca
     2701 tgcatggcct gctcaagccg tccaaccctg cgcaggcagg tttggagccg tggctttcgc
     2761 cctactcaga cgcgccgcca acggcccact cgggcgcgtc caactgagga atttaggcgc
     2821 gatgcgcatc aaacttacca acatgttgtc ggtctttgcc gactgtctcg ggcagcaggc
     2881 accccgtttt gaacctgttg tgggccatgg ctgttcgcca aaggcgaacc tcattttttc
     2941 cagcttgcct gggaaaatgc ctggtctcaa acaagtcaag tctttgcaaa tcatgcgaag
     3001 aactcaaaca ttcaaagtca ttcgtcgcga gggcgcgact cctacaaatt caaa
//
LOCUS       DAOSLL010000591         3042 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_28066_length_3042_cov_3.500167, whole genome shotgun sequence.
ACCESSION   DAOSLL010000591 DAOSLL010000000
VERSION     DAOSLL010000591.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28066_length_3042_cov_3.500167"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..580)
                     /locus_tag="PLB25_19445"
     CDS             complement(41..580)
                     /locus_tag="PLB25_19445"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HOE43780.1"
                     /translation="MIRLKFSVALNYTIAEPGSDFIFSVHAAQTPYQRVVNEVLSISQ
                     ALPRNAYTDPVTHTRFLRLKANAGALTVLYAATVDLDHYTAQPTQIAEVPVADLPGHV
                     LPYIYPSRYCQSDRLGRLAMKEFGSLWHGYGRVRAICDWVTQHVRFQSNSSNSTTTAV
                     DTLVDKVGVCRDFAHLQIS"
     gene            868..1152
                     /locus_tag="PLB25_19450"
     CDS             868..1152
                     /locus_tag="PLB25_19450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43781.1"
                     /translation="MTTRDTYINKMKLQLDDLNLQMDKLEAKAQEAREDVRDTYRAEM
                     AKVHAQSKIAMAKLEELQASGESTWDAMVAETEKIRDAFVHSFRYFKSQV"
     gene            complement(1171..2127)
                     /locus_tag="PLB25_19455"
     CDS             complement(1171..2127)
                     /locus_tag="PLB25_19455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009666394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HOE43782.1"
                     /translation="MPNTSASKPAPDLLAASQPRRRGWRHWVAWLARWPGWQLVTQVT
                     RSWLDDYAPSMGAALAFYTLFSLAPLLLIVVSVAGMAFGEDAARGEIAHQLQSLMGAR
                     AAASVQDVLVSVDQPAQGLLATLLGFFLLLIGATTVFGELQDALDRIWRVPTANRSNG
                     WLGLVRARLLSFGMILAVGFLLTVSLLLSAGLAMMGRWWMPLFGGWYAMAEVANGIGS
                     FVLNAVMFALIYKVMPRVNVPWRDVWTGAIFTTLLFSLGKSLMGAWIGSSSIVSGFGA
                     AGSLVLVLLWVYYSAQVFLLGAEFTWVYANLFGSRKALQSPA"
     gene            complement(2093..3007)
                     /locus_tag="PLB25_19460"
     CDS             complement(2093..3007)
                     /locus_tag="PLB25_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43783.1"
                     /translation="MSADHRKLAPALKPWCLWLLLAVTLPVAAVPTAPVADAGVALHS
                     QHTRLRAQLAQNVFQHPLVLQSHETAQGLQGDAYAEVAFAFEAVSAALRQPDQWCEVM
                     ILHINTKYCRASSGPTGALLNVNIGTKTAQELAQSARLAFSFSVLAATPDYFEVLLDA
                     AEGPLGTSDYRIRLEAIALGQQRTFLHLTYAYSVNLLGRVAMQTYLATVGADKVGFTP
                     KDNGAGAAPTLVNGVRGLVERNTMRYFLAIDSYFQATPGAPAAQFEQRLQSWFTATEA
                     YPRQLHETDRAAYLVMKHAEYQRQQASP"
BASE COUNT          649 a          966 c          901 g          526 t
ORIGIN      
        1 tttcgtttgg tctgatgtgc cattcacggt tccttgcgga tcaggaaatc tgcaggtgcg
       61 caaaatcccg gcaaacgccg accttgtcca ccaaggtgtc aaccgccgtg gtggtgctgt
      121 tggagctgtt ggattgaaac ctcacatgct gggtcaccca gtcgcaaatg gcccgcaccc
      181 ggccataacc atgccacagg ctgccaaact ccttcatggc cagacggccc agccggtcgg
      241 actggcaata gcggctgggg tagatgtagg gcaacacatg gccgggcaag tcggccaccg
      301 gcacttctgc aatctgcgtt ggctgcgcgg tgtagtgatc gaggtccacc gtggcggcgt
      361 acaacaccgt cagcgctccc gcgttggctt tgaggcgcaa aaaccgggtg tgcgtgaccg
      421 ggtcggtgta cgcgttgcgc ggcagcgcct ggctgatgct caaaacctcg ttgaccaccc
      481 gttggtacgg cgtttgtgct gcatgcacgc tgaaaatgaa gtcactgcca ggctcggcaa
      541 tggtgtagtt caacgccacc gagaacttca atcggatcat gaatgtcttt caaaaaaaag
      601 caccccacct ttgacaagtt ggcgtgacag atgccaacag gggcgcaccg gggacagggc
      661 tggcctgggc tttggtataa gttctggcgc atggcgtgtc tgtgcgttgg ctgacaaatc
      721 tgccccgttg gcaatgtgtg caagcgcaca gaccctggca ccgtccgggc ttaagctggc
      781 aggcacatga cggcaaccat gccattgatg cctgtcatca accggccaat cccggcccac
      841 aaccgttgcc acacaggagc agtcaccatg accacccgcg acacctacat caacaagatg
      901 aaacttcaac tcgacgacct gaacctgcaa atggacaagc tcgaagccaa ggcgcaagag
      961 gcgcgtgaag acgtgcgcga tacctaccgc gcagaaatgg ccaaggtcca cgcccagtcc
     1021 aagattgcca tggccaagct cgaagaactg caggccagtg gcgagagcac ctgggatgcc
     1081 atggtggccg agaccgagaa aatccgcgac gcatttgtcc attccttccg ctacttcaag
     1141 tcgcaggtct agcaccccca ggcacgcgct tcaggcaggg ctttgtagag cctttcttga
     1201 gccaaacagg ttggcataca cccaggtgaa ttcggccccc agcaaaaaca cctgtgccga
     1261 gtaatacacc cacagcagca ccagcaccag cgagcccgcg gcaccaaaac ccgacacgat
     1321 gctgctgctg ccaatccacg cacccatcag cgacttgccc aaagaaaaaa gcagagtggt
     1381 aaagatggcc ccggtccaga catcacgcca gggcacattg acccgcggca tcaccttgta
     1441 gatcagggca aacatcaccg cattgagcac aaagctgcca atgccgttgg ccacctcggc
     1501 cattgcgtac cagccgccaa acagcggcat ccaccagcgc cccatcatgg ccagaccggc
     1561 gctcagcagc aatgacaccg tcagcaagaa gccgaccgcc agaatcattc caaacgacaa
     1621 cagccgggcg cgcaccagcc ccagccagcc gttgctgcgg tttgccgtgg gcacacgcca
     1681 aatgcggtcc agcgcgtcct gcaattcgcc aaacaccgtg gtggcgccaa tcagcagcaa
     1741 gaaaaagccc agcagcgtgg ccagcagacc ctgggctggc tggtccacgc tgaccagcac
     1801 atcttgcacg ctggcagcag cgcgtgcgcc catcaggctt tgcagttggt gggcgatttc
     1861 gccacgggcc gcgtcttcgc caaacgccat gcccgccacc gacaccacaa tcagcagcag
     1921 cggcgccagc gaaaacaggg tgtaaaacgc cagcgccgca cccatgctgg gggcgtaatc
     1981 gtccagccac gagcgtgtca cctgcgtcac cagttgccaa ccgggccagc gtgccagcca
     2041 ggcgacccag tgccgccaac ctcgccgccg gggctggctg gccgccagca agtcaggggc
     2101 tggcttgctg gcgctggtat tcggcatgtt tcatcaccag gtaggcggcg cggtcagttt
     2161 cgtgcagttg ccgtgggtag gcttctgtgg cggtaaacca gctttgcaag cgctgctcaa
     2221 actgtgctgc gggtgcgcct ggtgtggctt gaaaatagct gtcaatcgcc aggaaatagc
     2281 gcatggtgtt gcgctcgacc agcccgcgca caccgttgac cagcgtcggt gctgcccctg
     2341 cgccgttgtc ttttggcgta aagccgacct tgtcggcgcc cactgtggcc aggtaagtct
     2401 gcatcgccac acggcccagc agattgactg aatacgcata ggtcaggtgc aaaaaggtgc
     2461 gctgctgccc cagcgcgatg gcttccaggc ggatgcggta atcgctggtg cccagcgggc
     2521 cttcggcggc atcgagcagc acttcaaaat agtcgggagt ggctgccagc acactgaagc
     2581 tgaacgccag ccgtgccgac tgggccagtt cctgcgctgt cttggtgcca atgttgacgt
     2641 tcagcagtgc gcctgtcggc ccgctgctgg cgcggcaata cttggtgttg atgtgcagga
     2701 tcatcacctc gcaccactgg tcaggctggc gcagggcggc actgactgcc tcaaacgcaa
     2761 acgccacttc ggcgtaagca tcaccttgca agccttgtgc ggtctcgtgg gactgcagca
     2821 ccagcgggtg ctgaaagacg ttttgcgcaa gctgcgcgcg caggcgtgtg tgctgactgt
     2881 gcaacgctac gcctgcatca gccacaggcg cagtgggcac cgccgcaacc ggcagggtca
     2941 ccgccagcag cagccaaagg caccacggct tgagcgccgg ggccagcttg cggtgatcag
     3001 ccgacatggt cgcggttcag gtttgacagc agggcatcgt gg
//
LOCUS       DAOSLL010000592         3035 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_28170_length_3035_cov_5.027181, whole genome shotgun sequence.
ACCESSION   DAOSLL010000592 DAOSLL010000000
VERSION     DAOSLL010000592.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3035
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28170_length_3035_cov_5.027181"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..929)
                     /locus_tag="PLB25_19465"
     CDS             complement(90..929)
                     /locus_tag="PLB25_19465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012345048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43784.1"
                     /translation="MSHRTNRTNRTNPQAAAPSSFARHLWFGQTAKAAAATSAVLSAA
                     LLLGACATTPAPPDWQANAFAALNSYTAAYLAGNSRIAEFEFRRARAEVARTGRPDLV
                     ARVELVRCAAQVASLDFSACTGYQALAADALVAEQAYAQFLTGRWVGLQPELLPLQYR
                     SLVQQWQASSARPAVSPAATAAVSVASSANGLAATGMSADSLRQMEDPLARLIAAGAL
                     LKAELLAPADIGLAAETASNQGWRRALLAWLGVQQQRALAAGDASTSASLQRRIDLLL
                     QTP"
     gene            complement(926..1894)
                     /locus_tag="PLB25_19470"
     CDS             complement(926..1894)
                     /locus_tag="PLB25_19470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020230252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HOE43785.1"
                     /translation="MNDCPPPPDPVTAPLASLERKAVALLTLVLLIIAGFVLYVMFAR
                     GVFERTRQLVLLSDDSEGVIVGMDMTFSGFPVGRVQRIELADDGKVRIVMEVPEKDAR
                     WLRTSSIFTLERGVVGDTRIRAFSGILTDPPLPANAERTLLRGDMAAEIPRLVASVRA
                     LLENLETMTGSDSSINTSLAHLKIVTARLTGRYGLLTGALGSEENAKKLITTLDSANA
                     LLGKADRRVFGAGGVVDSTQATLAGSQAAIAQLTALLADTRVTLTKVDAVLLEAQAVG
                     ANARTASVDLGILRAEVEASLRKISHLTDEINRKWPFARDTEIKLP"
     gene            complement(2107..2904)
                     /locus_tag="PLB25_19475"
     CDS             complement(2107..2904)
                     /locus_tag="PLB25_19475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011808352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE43786.1"
                     /translation="MSSSLTLTSSLKSLPCALRRQLDSVWFMLKFGAVLLVLALTPST
                     HTAAYRANTARRMYTSTWQVLPWFMALSALLSLVLIRIVVVTALSYGLSQYALQMVVR
                     VLVLELIPLSAAMFVALRAGLAFNIGAPGVAAGGRVSKVSELALDDLRTELTPRALAD
                     AFSVLALAMVSSVVALALAYLMVYGLSPWGLPGFTRAVGQVFDLTVSLSFVLKAFFFS
                     MAVAVVPLAASLEAVAPASQSGIQPGAVRLFLVLLLLEAASLIVKYL"
BASE COUNT          593 a          923 c          980 g          539 t
ORIGIN      
        1 ttaatattta agcagtaaga ggtggcgttt tggtggggtt tccgttctgg cactaggtag
       61 gctgttgcgg ccggttcgca gcgcaacggc tacggggttt gcagcaacag atcgatgcgt
      121 ctttgcaggc tcgccgaggt gctggcatcg cctgcagcca aggcccgctg ctgctgcacg
      181 ccaagccacg ccaggagcgc tcggcgccac ccctggtttg atgccgtttc agccgccagc
      241 ccaatatcag cgggcgctag cagctctgct ttcaagagcg ccccggcggc aatcaggcgc
      301 gccagcggat cttccatctg gcgcaatgaa tcggctgaca tgccggttgc ggcgagcccg
      361 tttgcgctgc tggcaacaga aactgctgca gtggctgccg gagaaactgc cggacgcgcc
      421 gaactggcct gccactgctg caccagactg cggtactgca gcggcagcaa ctcaggctgc
      481 aggccgaccc agcggccggt cagaaattgg gcataggctt gctcggctac cagggcatct
      541 gctgccagtg cctgataacc ggtgcaggcg ctgaaatcga ggctggccac ttgcgcggcg
      601 cagcgcacca gttcaacccg tgccaccagg tcagggcggc cggtgcgggc aacttcggct
      661 ctggcgcgcc tgaattcaaa ctcggcgatc cggctgttgc cagccaggta ggcggcggtg
      721 tagctgttga gtgcggcaaa cgcattggct tgccagtcag gtggggccgg tgtcgtggcg
      781 caggcaccca gcagcagcgc agcagacaac acagcgctgg tagccgctgc ggcctttgcc
      841 gtctggccaa accaaaggtg gcgggcgaag ctgctcgggg cagcggcctg gggattggtg
      901 cgattggtgc gattggtgcg atggctcatg gcagcttgat ctccgtgtca cgcgcaaatg
      961 gccatttgcg gttgatctcg tcggtcaagt ggctgatctt gcgcaggctg gcctcaacct
     1021 cggcgcgcaa gatgcccagg tcaacgctgg cagtgcgcgc gttggcaccg accgcctgcg
     1081 cttccagcag cacggcatcc accttggtca gggttacccg ggtgtcggcc agcagcgcgg
     1141 tcaattgggc aatggccgcc tggctgccag ccagcgtcgc ctgggtgctg tcaaccacgc
     1201 cgccggcacc aaagacgcgc cggtcggcct tgcccagcag cgcattggca ctgtcgagcg
     1261 tggtgatgag ttttttggcg ttttcttcgc tgcccagcgc accggtcagc agcccgtagc
     1321 ggccggtcag acgggcggtg acgattttca ggtgcgccag actggtgttg atgctggaat
     1381 cagagccggt catggtttcc aggttttcca gcaaggcgcg caccgatgcc accaggcgcg
     1441 gaatttctgc cgccatgtcg ccgcgtaaga gcgtccgttc ggcattggca ggcagcggcg
     1501 ggtcggtcag gatgccgcta aaggcgcgaa tgcgggtgtc gcctaccacg ccgcgctcca
     1561 gcgtgaagat gctggaggtg cgcagccagc gcgcgtcctt ttcgggcacc tccatcacaa
     1621 tgcgcacctt gccgtcgtcg gccagctcga tgcgctgcac ccgcccgacc ggaaagccgg
     1681 aaaaggtcat gtccatgccg acaatgacac cctcggagtc gtctgacagc agcaccagtt
     1741 gccgggtgcg ttcaaacacg ccacgggcaa acatcacata cagcacaaag ccggcaatga
     1801 tcagcagcac cagcgtcagc aaggccactg ccttgcgctc cagactggca agcggggcgg
     1861 ttaccgggtc gggcgggggc gggcaatcgt tcatgctggg cttgaacctg agtttttcag
     1921 ttgacagctt gttttcattg gttgctcctt gtgtgcattt agtatttttt tcgacagcgt
     1981 tcactcagtg ggctacggca ccaggcactg gctggaaccg ctggcaacgg gcatgggggc
     2041 gtggctgctg gctggtttgc gtccaactga ggaatctggc ttgaatgtca tgtttgggca
     2101 ggtttttcag aggtacttga cgatcagcga agccgcttcg agcagcagca agaccagaaa
     2161 caaacgcact gcgccgggtt gaatgccgct ttgcgacgcg ggggcaaccg cttccagact
     2221 ggccgccagc ggcaccacgg caaccgccat gctgaagaaa aaggctttca aaacaaagct
     2281 cagtgacacc gtcaggtcaa acacctgacc aaccgcgcgg gtaaaacctg gcaaacccca
     2341 gggcgacagg ccatacacca tcaggtaagc cagcgccagc gccaccacgc tgctgaccat
     2401 ggccaatgcc agcaccgaaa aagcatcggc cagagcgcgc ggggtcagct cggtgcgcag
     2461 gtcatcaagc gccagttcag acactttact cacccgcccg ccagcggcaa caccgggtgc
     2521 gccaatgttg aaagccagcc cggcgcgcag cgcgacaaac atcgcggctg acagcggaat
     2581 cagctccagc accagcaccc gcaccaccat ctgcagcgcg tattgcgaca ggccatagct
     2641 cagggcggtg accaccacga tgcgaatcag caccaggctc agcaaggccg acagtgccat
     2701 aaaccagggc aacacctgcc aggtgctggt gtacatgcgc cgcgccgtgt tggcgcggta
     2761 ggctgcggtg tgagtggaag gggtgagggc cagcaccagc agcaccgcgc caaatttcag
     2821 catgaaccag acgctgtcca actggcggcg caaggcacag ggcaagcttt tcaatgacga
     2881 ggtcagggta aggctggaag acatcgggcg atgataaagc gtgcgtcgcc gggcttgccg
     2941 atcagcgatg gtcaatcccg tggctgctat cgcaattgag gtaaggatgt ggtcgggaat
     3001 aactccccgt tttgcaccaa ggagttggga tgcac
//
LOCUS       DAOSLL010000593         3034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_28191_length_3034_cov_3.561934, whole genome shotgun sequence.
ACCESSION   DAOSLL010000593 DAOSLL010000000
VERSION     DAOSLL010000593.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28191_length_3034_cov_3.561934"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..824
                     /locus_tag="PLB25_19480"
     CDS             225..824
                     /locus_tag="PLB25_19480"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008250675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HOE43787.1"
                     /translation="MNFWDQRFAEPGYKYGTEPNAFLLQQAARLAPASQVLVPGDGEG
                     RNGVWLAQQGRRVTSVDSSAVGLQKANALAASRGVALTTLQVDLADWSPEAASFDAVV
                     LVFTHLPQHIRQSAHRRLAQGLRTGGLLLLEAFHPDQLSHASGGPKDAAMLYLPALLE
                     ADFGAMLTPLLAWHGEVMLAEGAGHQGLACVTRWIGQKI"
     gene            912..1292
                     /locus_tag="PLB25_19485"
     CDS             912..1292
                     /locus_tag="PLB25_19485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HOE43788.1"
                     /translation="MKTAHDLVLSAKAQVHELLVEQAVDAIRDADVLVDVREADEYAA
                     GHIGGAVHISRGILEFRFSSNPSLQARDLKVVLYCKTSGRAALAAVALQDMGYLNVKS
                     IVGGFDAWVGAGKPVARPEQPVFE"
     gene            1591..1941
                     /locus_tag="PLB25_19490"
     CDS             1591..1941
                     /locus_tag="PLB25_19490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014428408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HOE43789.1"
                     /translation="MSTSPLLSIGRLALLAGLMGVVSPLCAQAVDVAAAEKLIEDSKC
                     GKCHSVDKQKDGPSFKKTAAKYKGKADAESKLVTHMTTGPSVEIDGVKEEHTKIKSKD
                     DAAIKNVARYILSR"
     gene            1956..2558
                     /locus_tag="PLB25_19495"
     CDS             1956..2558
                     /locus_tag="PLB25_19495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HOE43790.1"
                     /translation="MPFRMRPFFKWMRRHWLLTIGLLVVLLGFSVSVGISGAYVLAFT
                     STEEFCVGCHEMSYNYAEYKGTIHDVNRTGVRAICTDCHVPHEPGPLILAKIKATKDL
                     YYTYISPSIDTREKFEAKRAVMAQRVWRDMKASDSHQCRSCHREDKMSADLQTAKAKV
                     RHAKGKAEGKTCIECHFAIAHKEPEGPGPAELFGEAAAKP"
     gene            2588..>3034
                     /locus_tag="PLB25_19500"
     CDS             2588..>3034
                     /locus_tag="PLB25_19500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
                     /protein_id="HOE43791.1"
                     /translation="MKLSLLAAVVSASIVLPAMAADVTYRNDIKPFIKAQCAECHAGD
                     APTLQEFKLNEKKYIDDKLGPRTDTYENLLAIVAYPDHGAFMRRLDDGSNTADKKPGN
                     MYKHLGETDAERAKALLMFKAWVGEGGWNLNRWDAKGDVPGVTKEQL"
BASE COUNT          671 a          811 c          901 g          651 t
ORIGIN      
        1 atgcgcagcc aatggcggcc tgaacacgaa ggggggaact acgcactgtc cagtctaaca
       61 ggtcaaatct ttgcacacca tgcgaagaat tcaaatattc aaagtcattc gccgcgaggg
      121 cgcgactcct gcacattcaa ataggtgccg acggtttgtg caggagtcgc gccctcgcga
      181 cgaaggctca tcgattccgg ctcgtccggc taaggggttc aaatatgaat ttttgggatc
      241 agcggtttgc cgaaccgggc tacaaatacg gaaccgagcc caacgcgttt ttactgcagc
      301 aggccgcgcg tctggcccct gccagccagg tgctggtgcc aggcgatggc gaaggacgca
      361 atggggtctg gctggcacag caggggcgcc gcgtgacctc tgtcgatagc tctgccgtgg
      421 gcctgcaaaa ggccaatgcg ctggctgcca gccggggggt cgctttgacc accctgcagg
      481 tggatctggc cgactggtcg cccgaagctg cgagctttga tgcggtggtg ctggtattta
      541 cccatttgcc gcagcacatt cgccaaagcg cccaccgccg actggcgcag ggtttgagga
      601 ccggtggcct gctgctgctg gaagcttttc acccggatca gttgagccat gccagtggtg
      661 gcccgaagga tgcagcaatg ctttacttgc ccgcgctgct ggaagcagac tttggcgcta
      721 tgctgacccc cttgctggcc tggcatggtg aagtcatgct ggctgagggt gccggtcatc
      781 agggcctggc ctgcgtgacc cgctggatag gtcaaaaaat atgaatgaaa tcagccgcca
      841 gcccaatcag cataagcgct gacagctatg tttatagtag tattaatgat aacatctatc
      901 aggagaattt catgaaaaca gcccacgatc tggttctttc cgccaaggcg caggtgcatg
      961 aattgttggt tgaacaggcc gttgatgcga ttcgcgacgc tgacgtgctg gttgatgtgc
     1021 gcgaggccga tgaatacgcc gccggtcata ttggcggtgc cgtgcatatt tcacgcggca
     1081 tccttgagtt caggttcagc agcaacccgt cgttgcaggc ccgcgacctg aaagtggtgc
     1141 tgtactgcaa aaccagtggc cgtgctgcgc tggcggcggt tgcgctgcag gacatgggtt
     1201 atctgaacgt gaaatcgatc gtcggtggct ttgatgcctg ggtcggcgcg ggcaaaccgg
     1261 tggccaggcc cgagcagcca gtgtttgagt gaggcagcag gtgctggcgc gttgaccgga
     1321 tcgccccgtg ctgcataaaa ttcagtcatt tatgccgctg tcctttatgg aataagcgct
     1381 gattgctatc aggtagatag taaatcgatg cttgcactcg gcctgttttg gtgaaaaagc
     1441 aggcccgaag ttattgtctg agttgttgtt cttcctcagc ttaaagcgat cttgattgat
     1501 ttaaatcaat cgaatgtgcg ccgttcactg gtttttcgca tagcatgcgt cagggatagg
     1561 caaaaacttc aacaacaaca ggaaacattc atgtctacct ctcccttgct ttctattggt
     1621 cgtttggcgc tgctggccgg attgatgggt gtcgtgtcgc cgctttgcgc ccaggccgtg
     1681 gatgtggccg ctgccgagaa attgatcgag gacagcaaat gtggcaaatg ccattcggtt
     1741 gacaagcaaa aggatggccc ctcattcaag aagacagccg ccaaatacaa gggcaaggcc
     1801 gatgccgagt ccaaactggt gacccacatg accaccggtc caagcgttga aattgatggt
     1861 gtcaaggaag agcacaccaa gatcaagtcc aaagacgacg ctgccatcaa aaacgtcgcc
     1921 cgctacatct tgtcacgtta gtcagcgctt acagcttgcc attcaggatg cggccgttct
     1981 tcaaatggat gcgtcggcac tggctgttga cgatcgggct gctcgttgtg ctgctgggct
     2041 tttcagtctc ggtgggtatt tcgggtgctt atgtgctggc gtttaccagt accgaggagt
     2101 tttgcgttgg ctgccatgaa atgtcctaca actatgccga gtacaagggc accatccatg
     2161 atgtcaaccg caccggtgtg cgggccatct gtaccgattg ccatgtgccg cacgagcccg
     2221 gccccttgat tctggccaag atcaaggcca ccaaggacct ttattacacc tacatttcgc
     2281 cctccatcga cacccgcgag aagtttgagg ctaagcgcgc ggtcatggcg caaagggtgt
     2341 ggcgggacat gaaggcgagt gactcacacc agtgccgcag ttgtcaccgc gaagacaaga
     2401 tgagtgccga cttgcagacc gccaaggcca aggtccgcca tgccaagggc aaggccgagg
     2461 gcaagacctg catcgagtgc cactttgcaa ttgcccacaa ggagccagag ggcccgggcc
     2521 cggcagaatt gtttggcgag gcggctgcaa agccctgagc gtgtcgttta tcaaccgagg
     2581 agaaaaaatg aaattgagtc tattggctgc cgtggtcagc gcatcaatcg tcttgccagc
     2641 catggctgcg gatgtgactt accgaaatga catcaagccg tttatcaagg cccagtgtgc
     2701 cgaatgccat gcgggtgatg cgcccacctt gcaggaattc aagctcaacg agaaaaagta
     2761 catcgacgac aaactcggcc cgcgtaccga tacctacgaa aacctgctgg cgatagtggc
     2821 ctatccggac cacggtgcgt tcatgcgtcg cctcgacgac ggcagcaata ccgccgacaa
     2881 aaagccgggc aatatgtaca aacatctggg cgaaaccgat gccgagcgcg ccaaggccct
     2941 gttgatgttc aaggcctggg tgggcgaggg tggctggaac ctgaaccgct gggatgccaa
     3001 aggtgatgtg cccggtgtca ccaaagagca gctt
//
LOCUS       DAOSLL010000594         3020 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_28355_length_3020_cov_3.926813, whole genome shotgun sequence.
ACCESSION   DAOSLL010000594 DAOSLL010000000
VERSION     DAOSLL010000594.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28355_length_3020_cov_3.926813"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1591
                     /locus_tag="PLB25_19505"
     CDS             <1..1591
                     /locus_tag="PLB25_19505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43792.1"
                     /translation="RDVREFVDGLFVGADREDFGLFLPALSVDINISAEEKSCQNKSR
                     RFQEDAQAKLLGSVPAADGKSNQIAVPEKKEHVVEIDFGNPLGIPLVPDGLHVTPLPE
                     SIVLDRGELRLYVTSNNHDNSAQITRHPKNPEVWHLSLPPGATLRSSAPIIRAGAARL
                     FLLATLGRKKIEISNLLLRKHQPAETSDKHKKLSFPFDTPGNVLGAYFAFCLAVVVFC
                     VVLHPAVSLPGFGVLAWAVSRDIANSRTQLLHLASRLSVFWGPQIGLSIVSLMLGLWP
                     AAANLLHIDFLSAVLYGSIPLMFSFSLVALDFQPKEEKLAREKHEEIHHALSTHMHEI
                     TQTALSVALGNLVRPPQPHSYTYFPEMLMKQMDKEIGTIEAVDHFKKLIGDRLKTTEA
                     AVKKSHEGQQHARRAVMAAGGAIFTGYFAFEAGEKVIEFQDLQSKCDAISFFHWNQWN
                     SPYATKEEIQKNIEMTHPADSHTAMQVPGTHVCNSFEDFNQHQIHSTGLLLLATLVVS
                     LVTAIVTIRKPSDEHSGGGHH"
     gene            1629..2099
                     /locus_tag="PLB25_19510"
     CDS             1629..2099
                     /locus_tag="PLB25_19510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43793.1"
                     /translation="MASKSELQEFYRSVVKAVLNIDSHVDHAGDITFELPEHGAFYIL
                     IDAQEDPEYFMLVYPNFFTVTKENYLRSLIALNVVNGNNKVVKLSYQEKDNAGKMKAS
                     AEMFIAGPNEVPDKVLVAQILRRTIAAMVSGVKAFLHEMPSTDASNAKRGQKRW"
     gene            complement(2187..2753)
                     /locus_tag="PLB25_19515"
     CDS             complement(2187..2753)
                     /locus_tag="PLB25_19515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HOE43794.1"
                     /translation="MLYQIVSLLLEVVTGFLSGACLLRLYMQYQRIPVSARSGNPLGR
                     LVFALTDWIVLPLRRVVPSIGALDTASLVAAYLLQLLEFGLLWLLAGGAGGLYAVPIL
                     AGFGVLRMAISGMTGAVIVYAVLSWVSTQSPMVDVIERLVTPPLTPIRRVLPLVGGID
                     LSPLVLLVLLQIVAIVLGHLQASALLAF"
BASE COUNT          765 a          809 c          793 g          653 t
ORIGIN      
        1 gcgcgatgtt cgcgagttcg tcgacggtct gttcgtcggt gccgaccgcg aggatttcgg
       61 gctgtttctg cccgcccttt ccgttgatat caatatttcg gctgaagaga aatcatgcca
      121 gaacaaatca aggcggttcc aggaagacgc acaagccaag ttgcttggca gcgtgcctgc
      181 agcagatggc aaatccaacc aaatcgcggt tcctgagaaa aaggagcatg tcgtggagat
      241 tgattttggc aatccgctgg gaattccgct ggtccccgat ggcctgcatg taacgccgtt
      301 gcctgaatca attgtgttgg atcgggggga actgaggttg tacgtcacga gtaacaacca
      361 tgacaattct gctcaaataa cccgtcatcc aaaaaatccc gaggtatggc acctatcttt
      421 gccccccggt gccactttga gaagttcagc gccaattatt cgcgccggtg ccgctcggct
      481 gtttctattg gcaacacttg gtcgaaagaa aattgaaatc agcaatcttt tgctgcgcaa
      541 gcaccagccc gcggaaacat cggacaaaca caagaagttg tcgttcccgt ttgatacgcc
      601 aggcaacgtg cttggtgcct atttcgcatt ctgcctggcg gtcgtggttt tctgcgtggt
      661 actgcatccg gcagtatcct tgccaggttt tggcgtattg gcctgggcag tttcacgtga
      721 cattgccaat tctcgtaccc aattgctcca tttggcatca cgtttgagcg tcttctgggg
      781 tccgcaaatc gggctttcaa tcgtcagttt aatgttgggg ctttggcctg ctgcggcaaa
      841 tctgttgcac attgattttt tgtctgcagt tctatacggc tccattcccc tcatgttttc
      901 attttccctg gttgctttgg attttcagcc gaaagaagag aaacttgcgc gcgagaagca
      961 cgaggaaata caccacgcgc tgtcgaccca catgcatgaa atcacgcaaa ccgcgctgag
     1021 cgtggcattg ggcaatttgg tacgtcctcc gcaaccgcat tcctacacct atttccccga
     1081 gatgctcatg aaacagatgg acaaggaaat tgggaccata gaagctgtgg accacttcaa
     1141 gaagctgatt ggtgatcgac tcaaaacgac ggaggctgcc gtcaagaagt cgcatgaggg
     1201 tcagcaacat gcacgccggg cagtcatggc cgcaggtggt gcaattttta ccgggtattt
     1261 tgcttttgag gctggtgaaa aggtcattga attccaggat cttcagtcca aatgtgatgc
     1321 tatttcgttc ttccactgga atcagtggaa cagtccgtat gcaaccaagg aagagattca
     1381 aaaaaacatc gaaatgaccc atccggcaga ttcgcacact gccatgcagg ttcccggaac
     1441 gcatgtgtgc aactcttttg aagatttcaa tcagcaccag atccattcaa caggcttgct
     1501 gctgctggct acattggtgg tcagtttggt gactgccatc gtcacgatcc gcaaaccaag
     1561 tgatgagcac agcggtggcg gtcaccattg atataactgt tcagtaaaac gcaaccagga
     1621 gaatcagagt ggctagtaaa tctgaactgc aggaattcta tcgttcggtc gtaaaggcag
     1681 tgctcaatat tgattcgcat gtcgatcatg ccggtgatat cacatttgaa ttgcccgagc
     1741 acggggcttt ttacattttg attgatgccc aggaagaccc ggaatatttc atgctggttt
     1801 atccgaactt cttcaccgtg accaaggaaa actacctgcg ttctctgatt gctctgaacg
     1861 tggtcaatgg aaacaacaag gtggtcaagc tctcgtatca ggaaaaggat aacgctggaa
     1921 aaatgaaggc atctgcagag atgttcattg caggacccaa cgaagtacca gacaaggtgc
     1981 tggttgcgca gatacttaga cgcaccatcg cagccatggt gagcggggtc aaggcgtttc
     2041 tgcatgaaat gcccagcact gatgcaagca atgcaaagcg tggccaaaaa cgatggtgag
     2101 caggccgcaa gttcaatggc aggtaaccgg cggcgaacca gtggtctcga tagcggcgaa
     2161 gccgattcga cggggcagtc aaaacatcag aatgccaaca gcgccgatgc ctgcaaatgc
     2221 cccagcacga tagccacaat ttgcaacagg accagcagca ccagtggcga caggtcaatg
     2281 ccaccgacca gcggtagcac gcgccggatc ggtgtcagcg gtggtgtcac cagtcgctcg
     2341 atcacgtcaa ccatgggcga ctgggttgat acccacgaga gcacggcata aacaatcacc
     2401 gccccggtca tgccggagat cgccatccgc agcaccccaa aaccggccag aattggcacc
     2461 gcatacaggc caccagcgcc acccgccagc agccacaaga ggccgaactc aagcagttgc
     2521 aacaggtaag ccgccaccag actggcggtg tcgagggccc caattgaagg caccacacga
     2581 cgcagcggca agacaatcca gtcagtcagt gcaaacacca gccgccccag cggattgccg
     2641 gagcgcgccg acaccgggat acgctggtat tgcatgtaca agcgcagcag acaggcaccg
     2701 ctcaggaagc cggtcacaac ctcaagcaac aaggaaacaa tttggtacag catggcattg
     2761 ggtaagaaga tggcagtgcg ctgcatcata gcgacacaac ggccctttaa aatgcagggt
     2821 ttgcgtgctt gcaacggctt gaaagccttg gctttgccaa cgcaaaatgc cagatgcgcg
     2881 ttaccccccg ttacccttga ttgccctaca tgtctgttca aaccactgcc ccatcgctca
     2941 gcccaacccc gcaggatgaa aaccagttaa ttggggagcg ccgcgaaaaa ctcaaagcgc
     3001 tgcgcctggc ccaggccgag
//
LOCUS       DAOSLL010000595         3014 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_28424_length_3014_cov_4.338290, whole genome shotgun sequence.
ACCESSION   DAOSLL010000595 DAOSLL010000000
VERSION     DAOSLL010000595.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3014
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28424_length_3014_cov_4.338290"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(165..851)
                     /locus_tag="PLB25_19520"
     CDS             complement(165..851)
                     /locus_tag="PLB25_19520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HOE43795.1"
                     /translation="MKFVAYERSKQGTGASRRLRITGRTPGIVYGGKAEPQLIEVDHN
                     ALWHALKKEAFHASILTMDVGGAESNVLLRDVQVHPFKQLILHIDFQRVDATTKLHMK
                     VPLHFFGDDQSQAIKAEGCIANHVFSELDVLCLPGDLPEFIKVDLSGLKKGSSLHLSD
                     IELPKGVKAVTRGGDKNPVVVSVVVVSAAEAPAADAVVGDATAVKPAAAKPAAAKPAA
                     KAAAKPAAKK"
     gene            complement(949..1926)
                     /locus_tag="PLB25_19525"
     CDS             complement(949..1926)
                     /locus_tag="PLB25_19525"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HOE43796.1"
                     /translation="MQVDQPPEFMVFTGNANPSLAADIARYLKTELGAATVGRFSDGE
                     VTVEIRQNVRARDVFVVQSTCAPTNENLMELLIVVDALKRASAERISAVIPYFGYARQ
                     DRRPRSSRVPITAKVVANMLQAVGVARVLTMDLHADQIQGFFDIPVDNIYASPVLLGD
                     LKQKNYDDLIVVSPDVGGVVRARALAKQLDCDLAIIDKRRPRANVSEVMHVIGEIDGR
                     NCVIMDDMIDTAGTLVKAAEVLKRRGAKMVYAYCTHPIFSGPAIDRITHGVALDEVVV
                     TNTIPLSPSALACPKIRQLNVAPLIAETIHRIAQGKSVMSLFSDQNNLF"
     gene            complement(1990..2066)
                     /locus_tag="PLB25_19530"
     tRNA            complement(1990..2066)
                     /locus_tag="PLB25_19530"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2030..2032),aa:Gln,seq:ttg)
     gene            complement(2106..2771)
                     /gene="ispE"
                     /locus_tag="PLB25_19535"
     CDS             complement(2106..2771)
                     /gene="ispE"
                     /locus_tag="PLB25_19535"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HOE43797.1"
                     /translation="MSTPLPDDDLVLRAARVLQQVSGTRQGAHIVIDKRIPTQAGMGG
                     GSSDAASTLLALNRLWNLKIGLAELLTLGLQLGADVPFFLGGKNAFVTGIGEKLAPVA
                     VSPGRYLVIKPAQGVATGRIFSDPLLKRDSEPTIIAGFAADVDGFGRNDLQDIARQFC
                     PGIDLAVDWLGRQGLTGRMTGSGSAVFAKLLQDIVPDGLPESFQFKVCSSLPVHPLVG
                     WAV"
     gene            2782..2874
                     /locus_tag="PLB25_19540"
                     /pseudo
     CDS             2782..2874
                     /locus_tag="PLB25_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012763000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
BASE COUNT          670 a          902 c          827 g          615 t
ORIGIN      
        1 cccaatgggt gaaccccatc gaagctgaaa aagtcaatat tcatgaggac ttaccgatga
       61 aaacaagtgg tcaactgagg aatccgggag catggtcaga ttatgcgttg ctggttcaaa
      121 aaaaaggctc accgaggtga gcctgtaggt cgcccgcaaa cagactactt cttggctgcc
      181 ggtttggctg cggcctttgc ggcaggcttg gcagcagcag gcttcgccgc agcaggcttg
      241 accgctgtgg catcacctac aacggcatct gctgcaggcg cttcagcggc ggacaccacc
      301 accactgata ccaccaccgg gttcttgtcg ccgccgcggg tgacggcctt cacgcccttg
      361 ggcagttcga tgtcggacag atgcagcgaa ctgccctttt tgaggccgct caaatccacc
      421 ttgatgaatt ccggcagatc gcccggcaag cacagtacat cgagttcgct gaaaacgtgg
      481 ttggcgatac agccctcagc cttgattgcc tgggattggt cgtcgccaaa gaagtgcagc
      541 ggcaccttca tgtgcaactt ggtggtggca tcgacacgct ggaaatcaat gtgcaagatg
      601 agttgcttga agggatgaac ctgcacatcg cgcaacagca cgttgctttc tgcaccgccc
      661 acatccatgg tcaaaatgct ggcgtggaag gcttcctttt tcagggcgtg ccacaacgca
      721 ttgtggtcaa cttcaatcaa ttgaggctca gccttaccac cgtaaacgat accgggcgtg
      781 cgaccggtaa tgcgcagacg gcggctcgca cccgtacctt gcttggaacg ctcataagcg
      841 acaaatttca ttttctaact cctggtaggg ccgaccgcga ccagtgcgac cctgatttaa
      901 aaaagcccgc cgtccagaag cggacctgcg agctactcgg cattctgctc agaacaggtt
      961 gttctggtca gaaaacaaac tcatgaccga cttgccctga gcaatgcggt ggatggtttc
     1021 ggcgatcagt ggtgccacat tgagctgcct gatcttggga caggcaaggg cgcttgggct
     1081 gagcggtatg gtgttggtga caaccacctc gtcaagtgcc acaccatggg taatacggtc
     1141 gatggccggg cccgaaaaga tcggatgggt gcaataggca tagaccattt ttgccccgcg
     1201 gcgcttaagt acctcggccg ccttgaccag cgtgccggcg gtgtcaatca tgtcgtccat
     1261 gatgacgcag ttgcggccat cgatctcgcc aatgacgtgc atgacttcac tgacgttggc
     1321 tctggggcga cgtttgtcaa tgatggcgag atcacagtcg agctgtttgg ccagtgcccg
     1381 tgcccgcaca acgccgccga catcgggcga caccacaatc agatcgtcgt agttcttttg
     1441 cttgaggtcg cccaacagaa ccggcgaggc gtagatattg tccaccggaa tatcgaaaaa
     1501 accctgaatc tggtcagcgt gcaaatccat cgtcaacacg cgcgccacgc ctaccgcctg
     1561 cagcatgtta gccaccactt tggcggtgat tggcacccgg cttgagcgcg gccgccgatc
     1621 ttgccgcgca taaccaaaat agggaataac ggcactgatg cgctcggctg atgcacgttt
     1681 gagcgcatca accacgatca gcagttccat caggttttcg ttggtcggcg cgcaggtact
     1741 ttgcaccaca aagacatcac gcgcacgcac gttttgccgg atttcaaccg tcacctcgcc
     1801 atcagagaag cggcctacag tggcggcacc caattcggtt ttcaagtaac gcgcgatatc
     1861 ggccgccaat gacgggttgg cattgccggt aaaaaccata aattcagggg gttgatcgac
     1921 ctgcatgtag atcccaacta gttagtcgca aacgcatcga catgtctgat aacaacatgc
     1981 atggaaattt ggcaggggag gaaggattcg aaccttcgca tgctggaatc aaaatccagt
     2041 gccttaacca gcttggcgac tcccctacgc gggtaaaacg cgaagcgcct taccgaaaaa
     2101 ccaacctata ccgcccagcc gacaagcgga tgaaccggca aactgctgca aaccttaaac
     2161 tgaaacgact caggtaggcc gtcaggcaca atgtcttgca acagtttcgc aaatacagcg
     2221 ctcccggaac ccgtcattcg accagtcaat ccctgtcggc ccaaccagtc aactgccagg
     2281 tcaataccgg gacagaactg cctcgcgatg tcttgcaagt cgttgcgacc aaaaccgtca
     2341 acatctgcag cgaagcctgc gattatagtg ggctcggaat ctcttttcag aagcgggtcc
     2401 gaaaaaattc ggcccgttgc caccccttgc gccggtttga tgaccaggta acgccccggt
     2461 gacacggcaa ccggggccag cttttcacca atgcctgtca caaaggcgtt tttgccgccc
     2521 agaaaaaaag gcacatcggc acccaactgc aatcccaggg taagcagttc tgccagccca
     2581 atcttcagat tccaaagccg attcaaggcc aacagggtcg atgccgcatc agatgagcca
     2641 ccgcccatgc ccgcttgcgt cgggatgcgt ttgtcgatga cgatgtgtgc gccttgccgg
     2701 gtgccgctga cctgctgcaa cacgcgtgca gcccgcagca ccaggtcgtc gtctggcagc
     2761 ggggtactca agtcttcccg gctgagctgg ctgctgctgc gcggcagatg ccgctggtgc
     2821 cgccagccga tctccgcccg ctacatcggc gtcgagctgc tcaccgccct tctcttctgc
     2881 gccgtcttcc tgcaggcggc gatcgaaccg ccgccgctgg ggatgacgcc gcttggccac
     2941 gaggcgctga tcccggtcac ctgggtcttc ctggcggcgc tggtctgcgg cgccttcgtc
     3001 gatttcgacc acat
//
LOCUS       DAOSLL010000596         3010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_28474_length_3010_cov_4.001692, whole genome shotgun sequence.
ACCESSION   DAOSLL010000596 DAOSLL010000000
VERSION     DAOSLL010000596.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28474_length_3010_cov_4.001692"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1114)
                     /locus_tag="PLB25_19545"
     CDS             complement(14..1114)
                     /locus_tag="PLB25_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HOE43798.1"
                     /translation="MPTHKTSAWPLALVYAGLIVYASLYPFAYWRDQGIASWHFLLAP
                     LPRYWTKFDVVTNVLGYVPLGFLLMLSALRTGRRRFALLLVVVAAAVLSFMMESMQVF
                     LPDRVPSNLDLVLNTSGGLLGAVVAWSLEKLGVLQRWGRFRAIWFIEEPRGALVLLAL
                     WPMALLFPAAVPFGLGQVMERLELALVELLADSQFLEWLPMREIELQPLTAGAEWLCV
                     ALGLLIPGLLGFCVIERRLKRAWFLVWIGFFGVGATGLSAAMSFGPEHTWAWLTGPVE
                     AALAAAALGLLSFLWLPRRACSALLVLALGIYLSLINQTSTSPYFEQTLFMWEQGRFI
                     RFHGLAQWLGWLWPFATLVYVLSLIWNKEPKN"
     gene            complement(1122..1550)
                     /locus_tag="PLB25_19550"
     CDS             complement(1122..1550)
                     /locus_tag="PLB25_19550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012202320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HOE43799.1"
                     /translation="MLWIKSFHIVFVASWFAGLFYLPRIFVNLAMVPVSSTAERERLL
                     LMSRKLLRFTTIIAIPAIGLGIWLWVVYGIGWGPGNGWMHAKLLAVAIVLGYHHACSA
                     MLKKFVRGEQRHSHVWYRWFNEAPVILMLIIVCLVVLKPF"
     gene            1775..>2535
                     /gene="hemB"
                     /locus_tag="PLB25_19555"
     CDS             1775..>2535
                     /gene="hemB"
                     /locus_tag="PLB25_19555"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOE43800.1"
                     /translation="MTPYAPYPLARPRRLRRDSFTRNLVREHAITSSDLIYPVFVVAG
                     KQQRQAVSSMPGVERLSLDLLLPVAEDCVKLGIAVMALFPVIDSCLKTPDGRDALNPD
                     GLVPRVIAALKKEFPELGIMTDVALDPFTSHGQDGLPDPRPEENGYIMNDATVEVLVK
                     QALTQAEAGADIVAPSDMMDGRIGAIRQALEAHHFVHTRIMAYSAKYASAFYGPFRDA
                     VGSAGNLGKGNKNVYQMDPGNSDEALREVAMDIAEG"
     assembly_gap    2536..2545
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2546..2763
                     /locus_tag="PLB25_19560"
     CDS             <2546..2763
                     /locus_tag="PLB25_19560"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007851161.1"
                     /note="catalyzes the formation of porphobilinogen from
                     5-aminolevulinate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HOE43801.1"
                     /translation="DVVRRVKDSFLIPTFAYQVSGEYAMLKAAAQNGWLNHDAVMMES
                     LLAFKRAGADGVLTYFARDAARLLLPL"
BASE COUNT          771 a          904 c          767 g          558 t
ORIGIN      
        1 catgcctgtg attttagttt tttggctctt tgttccaaat aaggctcagc acatacacca
       61 gcgtcgcaaa cggccataac cagcccaacc attgcgccaa cccatgaaaa cgaatgaatc
      121 gcccttgctc ccacatgaac agcgtttgtt caaaataggg actggtcgaa gtctggttga
      181 tcaggctcaa atagataccc aatgccagca ccagcagcgc tgagcaggca cgccgcggca
      241 gccacaaaaa tgacagcaga cccagcgccg ccgccgccag cgcggcttcc accggtccgg
      301 tcagccaggc ccaggtgtgc tctgggccaa agctcatcgc ggcgctcaag ccggttgcac
      361 caacgccaaa aaatccaatc caaaccagaa accaagcccg cttgaggcgg cgctcaatga
      421 cacaaaaacc caacaatccg ggaatcagca gccccagcgc cacgcacaac cactcagcgc
      481 cagcagtcag cggctggagt tcgatctcgc gcatgggcag ccattccaaa aactgactgt
      541 cagccagcaa ttcaaccagc gcaagctcga gacgctccat cacttggccc aaaccaaatg
      601 gcactgctgc cggaaacagc aaagccatcg gccacaacgc cagcaagacc aaagctccgc
      661 gcggctcctc gatgaaccag attgccctaa atcggcccca gcgctgcaac acgccgagct
      721 tttccagact ccatgcgacc accgcgccaa gcagaccacc tgacgtgttg agcaccagat
      781 ccaggttgga gggcacccgg tcgggcagga aaacctgcat cgactccatc atgaaagaca
      841 gtacggcagc ggccacgacg acaagcagca atgcgaaacg tctgcgtccg gtgcgcagtg
      901 cgctcaacat caacaaaaat cccagtggca catagcccag cacgttggtc acaacatcaa
      961 acttggtcca atagcgcggc aggggtgcca gcaaaaagtg ccaagaggca atgccttgat
     1021 ctcgccagta ggcaaaaggg taaagactgg cgtaaacaat caatcctgcg taaaccagcg
     1081 ccagaggcca tgccgaagtc ttatgcgtcg gcatgacagc ctcagaaagg cttgagcacc
     1141 accaggcaga caatgatcaa catcaaaatc accggtgcct cattgaacca gcggtaccag
     1201 acatggctat ggcgctgctc accgcgcaca aacttcttga gcattgcgct gcaagcatgg
     1261 tggtaaccca gcacaatggc cacagccagc aatttggcgt gcatccagcc gttgcctggg
     1321 ccccagccaa tgccataaac cacccaaagc cagatgccca agccaatcgc aggaatggct
     1381 atgatggtcg taaagcgcaa cagcttgcgt gacatcagta gcaaccgctc tcgctcggcc
     1441 gtggaactga ccggcaccat cgccagattc acaaaaatac gcggcagata aaacagaccc
     1501 gcgaaccaac tggcaacaaa tacaatgtga aacgacttga tccaaagcat gaaaacagtc
     1561 caatttgatg gcaatgggca aaaaaaaccc cagcacaagg gctggggtgg taagcctatt
     1621 tgctgtcaca catcaaggcc ccgctcaggg aggaaaagcg gggagcagta aactgcccgg
     1681 ggtctaattt aacaaagaat tgagcctgtt tcaagccagt gcgacaatta ttttgccttc
     1741 tcaatcaaaa tttcaaagaa atgagggacc ctgcatgact ccgtatgcgc cttacccgct
     1801 cgctcgtcca cgccgtttgc gtcgcgacag cttcacccgc aacctcgttc gcgaacatgc
     1861 catcacatcc agcgacctga tttatccggt ctttgttgtt gccggcaaac aacaacgcca
     1921 ggcagtttcc tccatgccgg gcgttgaacg tctgagtctg gacctgctgc tgccggtggc
     1981 agaggattgc gtcaagctag gcattgcggt gatggcgctg tttccggtga ttgattcctg
     2041 tctgaaaacc ccggatggtc gcgatgccct caatcctgat ggcctggtgc cgcgcgtgat
     2101 tgctgccttg aaaaaagaat ttcctgagct cggcatcatg accgatgtcg cactcgaccc
     2161 cttcaccagc cacgggcaag acggtctgcc tgaccctcgc ccagaggaaa acggctacat
     2221 catgaacgat gccacggtgg aggtactggt caagcaggct ctgacgcaag ccgaagccgg
     2281 ggctgacatc gtggccccca gcgacatgat ggatggccgc atcggtgcca tacgccaggc
     2341 gctggaagcc caccattttg tgcacacccg catcatggcc tacagcgcca aatacgccag
     2401 cgcgttctac ggcccctttc gcgacgcagt tggctcggct ggcaatcttg gtaaaggcaa
     2461 caaaaacgtt taccagatgg accccggcaa cagcgatgaa gccctgcgcg aagtagccat
     2521 ggacattgcc gagggnnnnn nnnnntggat gtggtccgtc gcgtcaagga cagcttcttg
     2581 atcccgacct ttgcctacca agtgtcgggc gaatacgcca tgctcaaagc tgccgcacaa
     2641 aacggctggc tcaaccatga tgcagtgatg atggaaagtc tgcttgcctt caagcgcgcc
     2701 ggcgccgatg gcgtgttgac ttattttgcc cgcgatgccg ccaggctgct tctaccttta
     2761 tagctactag cgctttatac ataattgtta gactgaagtt cttccccaaa aaaagccgcg
     2821 ttttcccaat gaaaatcagc caaatcagtg ttccgagttc tgactacatt ctgatctggg
     2881 cgatcaattc gagggcgcgt ttttgtgtgt ggttgggcgt tgtgatgatc tcaaaagtgg
     2941 gtgcatctgg cgcaccgcct ggcgtgcgac aggtattgcg cacgatggtt tgcaaagtgg
     3001 ccatcaaggt
//
LOCUS       DAOSLL010000597         3006 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_28522_length_3006_cov_4.300237, whole genome shotgun sequence.
ACCESSION   DAOSLL010000597 DAOSLL010000000
VERSION     DAOSLL010000597.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28522_length_3006_cov_4.300237"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            282..1388
                     /locus_tag="PLB25_19565"
     CDS             282..1388
                     /locus_tag="PLB25_19565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyldiacylglycerol synthase"
                     /protein_id="HOE43802.1"
                     /translation="MTCVDLIYFNAGGGHRAAARALKEAMQSEPWQTRSVNLFEMLDP
                     DGRFHDNFGLQPEDLYNKCLASGMTLGMSQELKLLQWLIRLSHKTMLRRMQAHWLATR
                     PDLVVSLIPNFNRALCQSLSAALPHVPFVTVLTDMADYPPSFWIEPDQPQHLVCGTEH
                     AVGQAMSAGCPAARVHQTSGMIISPHFYLPHVIDRVAERRKHGLDLQQPVGVVMFGGY
                     GSGAMKHIASSLPDTPLILICGRNAALAQSLRRAPSTAARVVVEFTDDVAYWMRLADF
                     FIGKPGPASLSEAVHMGLPVIIARNAWTMPQERWNTQWVSEHGLGVVVPSFAKVRAAV
                     DEIVSQLPQWQANVRKMQNRAVFEVPDILAKLLR"
     gene            complement(1413..>3006)
                     /locus_tag="PLB25_19570"
     CDS             complement(1413..>3006)
                     /locus_tag="PLB25_19570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HOE43803.1"
                     /translation="HENINYEFETHCVSVALDLWGNLSQPGRIFVNVSADVLLHLVGQ
                     CGFNSLLTMLTAMAVSPRMLVLEITEYERVADMDNLAAVVQEIRSAGVALALDDFGDG
                     RSSLRLWSQIKPEFVKIDKYFTKAISQHGDKLKTIQALQQIAAIFDTALIAEGIETED
                     DLRVLRDLGIRYGQGYFLGRPARLPREKIDMAAMAVIGDRTVAVFPELQRAPSAGSLS
                     RLAVIQAPTVNLLTTNDELARLLFKHPALHAIAVVDGTRPVAIINRAGFMNEYSKLYY
                     REIWGQKSCMFHANLEPRLIERNHNIDELVGILTSEDQRYLADGFIVTDNGCYQGLGT
                     GEQLVRSVTETRIEAARHANPLTFLPGNIPITQHIERLLASGVAFVACYADLNNFKPF
                     NDQYGYWRGDEMIRLLAKLAQLHCDPRRDFVGHVGGDDFILLYQSDDWQSQCQQIIDE
                     FAQQAIALFDAPAREAGGIQAEDRFGVTRFFPMTTLSIGAVEINPHHFANAEQVASVA
                     ALAKHHAKTDGKGLVIRSGPDR"
BASE COUNT          649 a          874 c          853 g          630 t
ORIGIN      
        1 tacgctggcc gctgccctgg gccttgccgt ggcggccttt gctgccgtcg gtcgcagcag
       61 caacatgatc atgccccaac ccagatgctg aatacgtttg gcaggtttgc aaacctttgg
      121 cagccccagc ctgcactggg tcaaagtgac agtcgatcca gccagtacca gcgtgcttca
      181 gcttgcaccg atgatcgtca agcagccaaa gtgggcttgc aaccaatgcg tcttgatcga
      241 acgccattgt cccggccggt cagggtcgaa cggctgccaa catgacctgc gttgacctca
      301 tctacttcaa tgcgggtggt gggcatcgag ccgccgccag ggcgctcaaa gaggccatgc
      361 aatctgaacc ctggcagact cgctcggtca atctgttcga aatgctggat ccagacggtc
      421 ggtttcacga caacttcggc ctgcaaccgg aagaccttta caacaaatgc cttgccagtg
      481 gcatgacgct gggcatgtcg caagagttaa agctgttgca atggttgatc cggctcagtc
      541 acaagaccat gctcaggcgg atgcaagcgc actggctggc aacccgaccg gacttggtgg
      601 tgtcgttgat tcccaatttc aaccgggcgt tgtgtcaaag cctgtctgcg gcgctgcccc
      661 acgtgccctt tgtgacggtg ctgaccgaca tggccgatta cccaccgagc ttctggatcg
      721 agcccgatca gccacaacac cttgtctgcg gtacagagca tgcagtcggt caagccatgt
      781 cggcaggctg ccccgccgcg cgcgttcacc agacctctgg catgatcatt tcgccgcatt
      841 tttatttacc gcatgtcata gatcgcgtgg cagagcggcg caagcatggg ctcgacctgc
      901 aacaacctgt cggtgtggtg atgtttggcg gctacggttc aggtgccatg aagcacatcg
      961 ccagcagctt gccagatacc cctttgatac tgatctgtgg caggaacgcc gctctggccc
     1021 aatcgctgcg ccgagcgcct tccacagccg ctcgggtcgt ggtcgaattt accgacgatg
     1081 ttgcctattg gatgcgactg gcggacttct ttattggcaa gccggggccg gccagcctca
     1141 gtgaggcagt gcacatgggc ttgccggtga tcatcgcgcg caatgcctgg accatgcccc
     1201 aagagcgatg gaacacgcag tgggtgagcg aacacggtct gggcgtggtt gtgcccagtt
     1261 ttgccaaggt gcgggccgct gtggatgaaa tcgtgagcca gttgccgcaa tggcaggcca
     1321 acgtcagaaa aatgcaaaac cgcgcggtgt ttgaagtgcc cgacatattg gcgaagttgc
     1381 tgcgctaaag cgccaaggca ggcgttgtca aatcaacggt ctggcccact cctgatcacc
     1441 agccctttgc cgtccgtttt ggcatggtgc ttggctaagg ccgcaaccga ggcaacttgt
     1501 tcagcattgg caaaatgatg tggattgatt tcgacggcac caattgacag ggtggtcatg
     1561 ggaaaaaacc gcgtgacacc aaagcggtct tccgcctgaa tgccgcctgc ctcgcgcgca
     1621 ggcgcgtcaa acaaggcaat cgcctgctgc gcaaattcgt cgatgatctg ctggcattgg
     1681 ctctgccagt cgtcactttg gtacagcaaa ataaagtcgt caccgcccac atgcccgaca
     1741 aaatcacgcc ttggatcgca atgcaactgg gccagtttgg ccagcaggcg aatcatttcg
     1801 tcgccacgcc aatagccata ctggtcattg aaaggtttga aattgttcaa gtcggcgtag
     1861 caggcgacaa acgcaacacc actggccaat agccgttcaa tgtgctgggt aatcggaata
     1921 ttgcccggca agaaggtcag cgggttggcg tgacgtgctg cctcaatgcg ggtttcagtc
     1981 actgagcgca ccaactgctc gccagtaccg aggccctgat agcagccgtt gtcagtgacg
     2041 atgaagccgt cggccagata gcgctgatcc tcggacgtga gaatgccgac caactcgtca
     2101 atgttgtggt tgcgctcgat caaacgcggt tcaaggttgg cgtgaaacat gcaagatttt
     2161 tgaccccata tctcgcggta gtacaacttg ctgtattcgt tcatgaatcc ggcacggttg
     2221 atgatggcca ccggacgggt gccgtccacc acggcaatcg cgtgcagtgc tggatgtttg
     2281 aagagcaacc gggcgagttc atcgttggtg gtgagcaaat tgacagttgg tgcctggatc
     2341 accgccagac ggctgagcga cccggcactc ggtgcacgtt gcaactcggg aaacacagcc
     2401 accgtgcggt cgccaatcac cgccatggcc gccatgtcaa tcttctcgcg cggcagccgt
     2461 gccggacgac ccaaaaagta accttgccca tagcgaatgc ccaagtcgcg cagaacgcgc
     2521 aaatcgtcct cggtttcaat cccctcggct atcagggcag tatcaaaaat cgcggcgatt
     2581 tgctgcagcg cctgaatggt cttgagcttg tcgccgtgct ggctgatggc tttggtgaag
     2641 tatttgtcga tcttgacaaa ctcaggtttg atttgcgacc acaggcgcaa gctggaacgt
     2701 ccatcaccaa aatcatcgag cgcgagtgcc acgccagccg agcggatctc ctggaccact
     2761 gccgccaggt tatccatgtc cgccacccgc tcgtactcgg taatctcaag caccagcatg
     2821 cgcggggaca ctgccatcgc cgtgagcatg gtcagcaaac tgttaaaacc acactgcccg
     2881 acaagatgca gcaggacatc ggcactgacg ttgacaaata tgcgacccgg ctggctcaag
     2941 ttgccccaca aatccagagc caccgagaca cagtgggttt caaattcgta gttgatgttc
     3001 tcatgt
//
LOCUS       DAOSLL010000598         2968 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_28973_length_2968_cov_3.414693, whole genome shotgun sequence.
ACCESSION   DAOSLL010000598 DAOSLL010000000
VERSION     DAOSLL010000598.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2968
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28973_length_2968_cov_3.414693"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(233..2005)
                     /locus_tag="PLB25_19575"
     CDS             complement(233..2005)
                     /locus_tag="PLB25_19575"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HOE43804.1"
                     /translation="MTVPIARILIIDDTPANLLVLCSVLDSEFELQFATSGPVGIALA
                     LKHPPDLILLDIMMPEMDGFEVCRRLKCNPVLKDIPVVFISALSEIESEYQGLTLGAV
                     DYIAKPVNVEIATRRISNLIEREVLRKDVMAQKKLLEESEAFKLAILNSLAEEIVVLD
                     KSGVIQAVNNPWYKFAQDNCLASGTLAQNIDVGSNYLEVCRKGISESSPDATLASGTL
                     RGIEAVLEGTLPCFSMEYPCHSPTQQRWFKMVVAPLQRSLHGGAVISHTDITERKVMQ
                     TRLIDSEAHLRAIVKNDPECIKIVNEDGILLQMNPAGLAMIEANTLEQVKGVPILQLI
                     APEHHQAFWAMHKRVIAGEHMQMEFEVIGLKGTRRWLETHAVPMQDHGQAVHLAVTRD
                     ITQRKQMEAQVLQLAFHDPLTSLPNRRLLLDRLSQAMTAGKRNGCYGALMFLDLDNFK
                     VLNDTHGHRVGDLLLLEVSDRLKACVREVDTVSRFGGDEFVVLLHDLGKQRPESTAQA
                     AIVAEKIRFTLQTVYSLSIGHEGGSVSEVEHHCTASIGVTIFFGHEVSEEHILKLADA
                     AMYKAKDAGRNTIRFADIPRTGTP"
     gene            complement(2002..2955)
                     /locus_tag="PLB25_19580"
     CDS             complement(2002..2955)
                     /locus_tag="PLB25_19580"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE43805.1"
                     /translation="MTDTGVGIPADKLDLLFKPFSQTDSSITREFGGSGLGLSIVSSL
                     AKLAGGDVGVQSEAGKGSRFWFRIRAGLVAIGTDSRREERCLVGVAPPEMSAFALSGH
                     VLVVEDNPVNCMVIEGLLDQLGVRVTLVNNGQQAVDAVTQGESLNAILMDLHMPVMDG
                     YTATEQIRLWEEDNQLPRLPIIALTADAFEADHQHCMAVGMDDFLTKPIVLDALKWTL
                     AKWLPDAPQKSTKNSVPLKPVDIDAFNALVSDLIPLLQDHKFTTIARFKALQKLAMDT
                     YLADELEVLASMLQEMRFDQVLTRLRLICFNHISVDKKVRS"
BASE COUNT          726 a          804 c          718 g          720 t
ORIGIN      
        1 gaataacggg gagaacttgg cactgtccag tttgctacat cagcaatcca tttcagccag
       61 ggttgaccag gtaggtcaac taatcacagc cactcttgac catcctgatg ggaaaaatca
      121 gaggacgaag attctttcaa ttgaccgcct gttgaacagc aaagaaatca gcagcaattt
      181 cctacgtttc gcctgctcac ttcaaggttg cgacaaaacc gtcagcggcg acttagggtg
      241 tccccgtccg gggtatatcg gcaaaccgaa tcgtattgcg cccagcatcc ttggctttgt
      301 acatcgccgc atcagccaac ttaaggatgt gctcctcgct cacttcatgg ccaaagaaaa
      361 tagtcacccc aatactggcg gtgcaatggt gctcgacctc actaactgag cccccttcat
      421 ggccaatgct caacgaatat acggtttgga gcgtgaaacg gattttttcc gcaactatcg
      481 cagcttgtgc tgtggattcc ggccgctgct tacccaaatc gtgcagtagc accacaaact
      541 cgtcaccgcc gaatcgagac acggtatcta cttcccgaac acaggctttc aatcgatctg
      601 agacctcaag taacaacagg tcgcccacac ggtgcccatg cgtatcattg agcactttga
      661 aattatccag atccaggaac atcaatgcgc catagcaacc gttgcgcttg ccagccgtca
      721 ttgcctgact tagacggtct aggagcaagc gtcgattcgg taaacttgtg agcggatcgt
      781 ggaatgcaag ttgcaacact tgagcttcca tttgctttcg ctgtgtgata tcacgggtaa
      841 ctgctaaatg cacggcctga ccatgatcct gcatgggtac agcatgggtt tccaaccaac
      901 gccgagtgcc ctttaggccg atcacctcaa attccatttg catatgttcg cccgcgatga
      961 cgcgtttatg catagcccaa aatgcctggt gatgttcggg tgcaatgagc tgaagaatgg
     1021 gtacaccctt gacctgctcc agggtattgg cctcaatcat tgccaaacct gcggggttca
     1081 tctgaagtag gatcccatcc tcattcacaa tcttgatgca ttccggatca ttcttaacga
     1141 tggcccgaag gtgagcctca ctgtcaatga gcctggtttg catcactttg cgttcagtga
     1201 tatcggtatg tgagatcact gcacccccat gtaaactgcg ctgaagcggt gccacaacca
     1261 tcttgaacca gcgctgttgc gttggtgaat ggcatggata ttccatgctg aagcaaggca
     1321 aagtgccttc gagtacagcc tctatgccac gcaatgtgcc tgatgccaag gtggcatcag
     1381 gtgaactctc tgaaatccct tttcggcaca cttcgaggta attgctgcca acgtcaatgt
     1441 tctgtgccaa tgtacctgac gcaagacaat tgtcctgagc gaatttgtac caaggattgt
     1501 tgactgcctg aatgactccc gacttatcca ggacaacaat ctcttcggcc agcgagttca
     1561 aaatggccag cttgaacgct tcgctttctt ccaacagctt cttttgtgcc atcacatctt
     1621 ttcgaagaac ttcacgttca atgaggttgc taatgcgtcg cgtggctatt tcaacattga
     1681 ctggcttggc aatgtagtcg acagcaccca gggtaagccc ttggtactcc gattcaattt
     1741 ccgatagcgc gctgataaac accaccggaa tatccttgag gaccgggttg catttgagtc
     1801 ttctgcatac ctcaaagcca tccatttcag gcatcatgat atcgagcagg atcaggtcag
     1861 gtgggtgttt cagggccaac gcaatgccta ccgggcctga cgttgcaaat tgcagttcga
     1921 actcactatc aagcactgag cacagcacta acaggttcgc tggcgtgtca tcaatgataa
     1981 ggattcgcgc gataggtact gtcatgatcg gactttctta tcaacgctga tgtggttaaa
     2041 gcagatgagg cgcagacgcg tcaacacctg atcgaaacgc atttcttgca gcatggatgc
     2101 caagacctca agctcatcgg ccaagtaggt atccatcgcc agcttttgca gcgctttgaa
     2161 gcgggcgatt gtggtgaatt tgtgatcttg cagcaaaggt atcaagtcgc tgaccaaggc
     2221 attaaacgcg tcaatatcga ccggcttgag cggcacactg ttttttgtgc tcttttgggg
     2281 agcatcgggc agccacttgg caagtgtcca tttcagtgca tctagaacaa tgggtttggt
     2341 caggaagtca tccatgccca ccgccatgca gtgttgatga tccgcctcga aggcatcagc
     2401 cgtcagtgca atgatcggca aacggggcag ctgattgtct tcttcccaaa ggcgaatctg
     2461 ttcggtggcg gtgtaaccat ccatcacagg catgtgtaag tccatcagaa tcgcattcag
     2521 agattcacct tgggtgacgg catcaacggc ctgctgacca ttatttacca gcgtcacacg
     2581 taccccaagc tgatccagca gaccctcgat caccatgcag ttaaccgggt tgtcttcaac
     2641 caccagcacg tgtccagata atgcgaaagc ggacatctca ggtggcgcga ctccaacaag
     2701 gcaacgctct tcccttcggc tgtcggtgcc tatggcaacc agaccagcac gaatgcgaaa
     2761 ccagaagcgt gagccttttc ccgcttcgct ctggacaccc acatcaccgc cggccagttt
     2821 ggccaagctg ctcacaatcg ataaaccgag tccggagccg ccaaattcac gtgtgatgga
     2881 actgtcggtt tgagaaaacg gcttaaaaag caagtcaagt ttgtctgcgg gtatgccgac
     2941 tccggtgtcg gtcaccgaga actccaat
//
LOCUS       DAOSLL010000599         2965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_29018_length_2965_cov_3.787285, whole genome shotgun sequence.
ACCESSION   DAOSLL010000599 DAOSLL010000000
VERSION     DAOSLL010000599.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29018_length_2965_cov_3.787285"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PLB25_19585"
     CDS             complement(<1..655)
                     /locus_tag="PLB25_19585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HOE43806.1"
                     /translation="MYSIKKAGLALALGAALLAQTALAQQQFVNVLTGGQSGVYYPLG
                     VALSQIYAKAIPNVRSTAQVTKASAENLNLLQAGRGELALALADSVSDAWKGDVEAGF
                     KTKLDKLRGLSATYNNYIQIVANADSGIKTLADLKGKRISVGAAKSGTELNARAILKA
                     AGLTYTDLGKVEYLPFGESVELMKNRQLDATLQSAGLGVASIRDLATAIKIIVIPVPA
                     "
     gene            complement(795..2471)
                     /locus_tag="PLB25_19590"
     CDS             complement(795..2471)
                     /locus_tag="PLB25_19590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE43807.1"
                     /translation="MALLEDDLSASSAMVVDSNPTSRSILVSQLREFGMGTVVQCARL
                     ADARRQLEYRKFDVVLCEHHFAGESSSGQDLLDDLRRNQLLPFSTVFIMVTGEATYTK
                     VAEAAESALDGYLLKPHKATHLGERLRQARIRKASLQEIFTAIEAADFDTAAALCLKR
                     FETRGKFWLYAARVGAELMLRVGKYDQAQKLYQAVVNAKTLPWAKLGVARAQLDEGQT
                     AKAVSTLENLISEDPNYTDAYDVMGRAQFEQGKFDAALATYKMASDMTPSSITRLQNL
                     GMMCYYSGEHKGAEKSLDRTVRLGLDSKMFDCQTLVLLAFVRLETGDHKGLQRCRDDF
                     ARLLERNPASGRHQRLAGTVEALNLIEQRQFARVLEVVRSICAQVKDPEFDYESASNL
                     VSLLAQLANKAILLDEVNAVIDTLGLRFAGTRTLAELLAGASSVHPPYAERIRAAHVQ
                     VLKLAENAISLSMGGNPRAAIETLVKHGSSTLSGKLIETAYLVLHRYAEKLTDVHELT
                     ELVQGLRSRYSTHITRAALGEQKRQAGGLTLRIDIKNGKAPATPAPAPAS"
     gene            complement(2580..>2965)
                     /locus_tag="PLB25_19595"
     CDS             complement(2580..>2965)
                     /locus_tag="PLB25_19595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HOE43808.1"
                     /translation="AIDRMLVDFPDADIVFIESGGDNLAATFSPELSDLTIYVIDVAA
                     GEKIPRKGGPGITKSDLFVINKTDLAPYVGADLEVMRADTTRMRTTAKGLRPFVMTNL
                     KTRAGLAEVIEFIETRGLLTGLLQT"
BASE COUNT          580 a          869 c          890 g          626 t
ORIGIN      
        1 cggcgggcac cggaatgacg atgatcttga tggcagtggc cagatcacgg atcgaggcga
       61 cacccagccc ggctgactgc aaggtggcat caagctggcg gtttttcatc agctcgaccg
      121 attcgccaaa cggcaaatac tcgaccttgc cgaggtcggt gtaagtcagc ccggcggctt
      181 tcagaatggc gcgggcattg agttcggtgc ccgatttggc agcgcccacc gaaatgcgct
      241 tgcctttgag gtcggccagc gtcttgatgc ccgagtctgc attggcgacg atctggatgt
      301 aattgttata ggtagcagac agaccacgca gtttgtcgag tttggtctta aaaccggctt
      361 ctacgtcacc cttccaggca tcggataccg agtcggccag cgccagtgcc agctcaccac
      421 ggcccgcttg cagaaggttg aggttttcgg cagaagcctt ggtgacctgc gcggtggagc
      481 gcacattcgg aatcgcctta gcatagattt gcgacagcgc cacccccagc gggtaataga
      541 caccactttg accgccggta agtacgttga caaactgctg ttgggccagc gccgtctggg
      601 ccaggagtgc tgcacccagc gccagcgcca gaccggcttt tttgatcgaa tacatgaaaa
      661 aatctcctgt gaatcgtgtt gtgaagcaag gcgggctgcg ggcgcagtcc agcaaactac
      721 ttcagtcagc cattatttga cgcaatcagg cccaggggcg ctagtgtttt cccgcgcgtc
      781 tgcccgcaaa cctgtcaact tgcgggtgct ggcgcaggtg tggcgggcgc tttgccgttc
      841 ttgatgtcaa tgcgcagggt cagcccaccg gcctggcgct tttgttcgcc cagcgcggca
      901 cgggtgatgt gggtgctgta acggctgcgc aggccttgca ccaactcggt caattcatgc
      961 acgtcggtca atttttcggc gtagcggtgc agcaccaggt aggcggtttc aatcagcttg
     1021 ccactcaagg tgctgctgcc atgtttgacc agggtttcga tggcggccct ggggttgccg
     1081 cccatgctca gcgaaatggc gttttccgcc agtttcaaaa cctggacatg tgctgcgcga
     1141 atgcgctcgg catagggtgg atgaacgctg gacgcgcccg ccagcagttc tgccagggtg
     1201 cgggtgccgg caaaacgcag ccccagggtg tcaatcaccg cattcacttc gtcaagcagg
     1261 atggccttgt tggccaactg cgccagcagc gacaccaggt ttgacgctga ctcatagtca
     1321 aactcagggt ctttcacctg agcgcaaata ctgcgcacca cctcaagcac gcgggcaaac
     1381 tgacgctgct cgatcaagtt cagcgcttca acggtgccag ccagccgctg gtggcggcca
     1441 ctggcgggat tgcgctcaag cagccgggca aagtcatcgc ggcagcgctg caagcctttg
     1501 tggtcaccgg tctcaagccg tacaaaggcc agcagcacca gcgtctggca atcaaacatc
     1561 tttgagtcaa gcccaagccg cacggtgcgg tcaagcgatt tttctgcccc tttgtgctca
     1621 ccagagtaat agcacatcat gcccagattc tgcaggcggg tgatggaaga gggtgtcatg
     1681 tcactggcca ttttgtaggt ggccagtgcg gcatcgaact tgccctgctc aaactgggcg
     1741 cgccccatca catcgtaggc atcggtgtag ttggggtctt cgctgatcag gttttccagc
     1801 gtgctgacgg ccttggcggt ctggccttcg tccaactggg cgcgggccac gccgagcttg
     1861 gcccagggca gcgtcttggc attgaccacc gcctgataca gcttttgcgc ctggtcgtat
     1921 ttgccgacac gcaacatcag ctcggctccg acacgggcgg cgtacagcca gaacttgccc
     1981 cgcgtctcga agcgcttcag acacagcgcc gccgcagtgt cgaaatctgc ggcctcgatg
     2041 gcggtgaaga tttcctgcag ggatgccttg cggatgcgcg cctggcgcag gcgctcaccc
     2101 agatgggtgg ctttgtgggg tttgagcagg tagccgtcaa gtgccgactc tgcggcttcg
     2161 gccaccttgg tgtaggtggc ctcgccggtg accatgatga acacggtgga aaaaggcagc
     2221 aactggttgc gccgcaagtc atcgagcaaa tcctgccccg agctcgattc accggcaaag
     2281 tggtgctcgc acaaaaccac gtcaaatttg cggtactcca gttgcctgcg ggcatctgcc
     2341 aggcgggcac attgcaccac ggtgcccatg ccaaattcgc gcaactgcga caccagaatc
     2401 gaccgtgaag tggggttgct gtcaaccacc atggcgctgc ttgccgacag gtcgtcttca
     2461 agcaaggcca tgtgaacccc ttggcaaatg tcggtgcggc cattgacggc gattcaatcg
     2521 atgctcctgt ctgattagcc gcaaactgta gcaccgcatg ccatgctgct gcagaaatat
     2581 tacgtttgca gcaagcctgt cagcaaacca cgggtttcga tgaactcaat tacctcagcc
     2641 aatccggccc gggttttcag gttggtcatg acaaagggcc tgagcccttt ggcagtggtg
     2701 cgcatgcggg tggtgtcagc gcgcatcacc tccagatcag cgcccacgta gggtgccaga
     2761 tcggttttgt tgatgacaaa caagtcgctc ttggtgatgc cggggccgcc cttgcgcggg
     2821 attttttcgc cggcggccac atcaatcacg taaattgtca ggtcggacag ttccgggctg
     2881 aaagtggccg ccaggttgtc gccaccactc tcgatgaaca cgatgtcggc atctggaaag
     2941 tccaccagca tgcggtcaat tgctt
//
LOCUS       DAOSLL010000600         2963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_29044_length_2963_cov_3.819464, whole genome shotgun sequence.
ACCESSION   DAOSLL010000600 DAOSLL010000000
VERSION     DAOSLL010000600.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2963
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29044_length_2963_cov_3.819464"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="PLB25_19600"
     CDS             <1..237
                     /locus_tag="PLB25_19600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2924 domain-containing protein"
                     /protein_id="HOE43809.1"
                     /translation="HSKIKSRQIARDIELAPGTVLLREWGDRDHKVTVTAEGTFDYEG
                     KFFKSLSAVARHISGTPWSGPLFFGLKTNRKEAT"
     gene            234..1613
                     /locus_tag="PLB25_19605"
     CDS             234..1613
                     /locus_tag="PLB25_19605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012080533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HOE43810.1"
                     /translation="MNVTSQIASPKPRKRCAVYCRVSSDERLEQEFNSIDAQKEAGQS
                     YVASQRSEGWIPVVDDYDDPGFSGGNTERPGLKRLMAEIERGLVDIVVVYKIDRLTRS
                     LADFSKMVEVFERQGVSFVSVTQQFNTTTSMGRLMLNVLLSFAQFEREVTGERIRDKI
                     AASKRKGLWMGGVPPLGYNVASRLLVVNETEAQLVRRIFGEMLTIGSPTRIAQGLASE
                     GITTKAWTTQDGQVRSGTQMDRKYLYKLLRNRIYLGELSHKGSWFPGAHAPIIDHGLW
                     GQVHEVLARDPHVRSVDTQTRESTEALLRGLLFGPSGDPMYPTYASKNKRKYRYYVSK
                     AEMRFGAAGKTHERIPAAEVEAATVNQVKTVLSSPEAITAVCKSLELKGVQINEDEIV
                     MGLHQLGEAWEQLYPAERIRIVKLMIERVDLVAGGLKVKWHALGWKELIKEFAAKGIG
                     AELVEMEAA"
     gene            1610..1972
                     /locus_tag="PLB25_19610"
     CDS             1610..1972
                     /locus_tag="PLB25_19610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase resolvase"
                     /protein_id="HOE43811.1"
                     /translation="MNADLESFVPLTYSRRGVQRLATTSTVAHDTTFLVGLSRAFYWK
                     SLLDTGVMKSGSEIAKNEGLHHSVVNELMRLTLLAPDIITQLMSGTQPRRLNLMWFQR
                     NRLPVDWQAQRAIIDGFK"
     gene            2059..2481
                     /locus_tag="PLB25_19615"
     CDS             2059..2481
                     /locus_tag="PLB25_19615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019560814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HOE43812.1"
                     /translation="METFIPWTLIKRGVRRQVITPIDAPEQFHREVVVERSARKEAKG
                     SPLIRALGLAHYWQRLLDEGKYRSLTEIAAAEGMDRGQANRIALLTRLAPEIIHTYLS
                     GGQANFALERLNPYGINLHWGEQIDQLQNSNLPSATRI"
     gene            2524..2877
                     /locus_tag="PLB25_19620"
     CDS             2524..2877
                     /locus_tag="PLB25_19620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43813.1"
                     /translation="MRLWLIVFLAILMPLQLSWAATGRYCQHESDVSSKHVGHHTHQH
                     QVTERTESGGDLAKTISVDMDCGTCHACCSVAVHEPDVVKKIALTLMLAPLPSVQSSP
                     GPEDRPERPQWDALV"
BASE COUNT          691 a          797 c          870 g          605 t
ORIGIN      
        1 cactccaaga tcaagagcag gcagattgcc cgcgacattg aactggcacc cggcaccgtt
       61 ctgctgcgcg aatggggcga ccgtgaccac aaggtgaccg tgaccgcaga gggcaccttt
      121 gattatgagg gcaagttctt caagagcctg tctgcggtgg ccagacacat ttcgggcacg
      181 ccatggtcag gaccactgtt ttttggcttg aagacaaacc gcaaggaggc aacatgaacg
      241 tcacctccca aattgcgtcg cccaaaccgc gtaaacggtg tgccgtgtac tgccgcgtct
      301 caagtgacga gcggctggag caggaattca actccatcga tgcgcagaaa gaggcaggcc
      361 aatcctatgt tgccagccaa cgctcggagg gctggattcc ggtggtggac gactacgacg
      421 accccgggtt ttctggcggc aacaccgagc ggcctggact caagcgcctt atggcggaaa
      481 tcgagcgcgg tctggttgac attgtggtgg tctacaaaat tgaccgcctc acccgcagtc
      541 tggctgactt ctccaaaatg gtcgaagtgt ttgagcgcca gggggtgtca ttcgtatccg
      601 tcacccagca gttcaacacc accacgtcca tgggccgctt gatgctcaac gtgctgctgt
      661 cgttcgcaca gtttgagcgg gaagtcaccg gcgagcggat ccgcgacaag attgccgcca
      721 gcaaacgcaa gggactgtgg atgggtggtg ttccgccctt gggctataac gtggccagtc
      781 ggctgttggt ggtcaacgaa actgaggctc aactggtgcg gcgcatattt ggtgaaatgc
      841 tcaccatcgg gtcgccgacc cggatcgcac aggggctggc cagcgagggc atcacaacca
      901 aagcctggac aacgcaggac ggtcaggtgc gcagtggcac ccagatggac cggaaatatc
      961 tttacaagct actgcgaaac cgcatctatc tgggtgagtt atcacacaag ggcagttggt
     1021 ttccgggcgc gcacgcgcca atcattgacc atggtctgtg gggtcaggtg catgaagtgc
     1081 tggcgcgcga cccgcatgtg aggtcagtgg acacacaaac ccgcgagagc accgaggcac
     1141 ttttacgtgg tctgctgttt ggccccagtg gcgacccgat gtacccgacc tacgccagca
     1201 agaacaaacg caaataccgc tattacgtct ccaaagcaga gatgcgattt ggcgcagcgg
     1261 gcaaaacgca cgagcgcatt ccggccgcag aggtggaagc ggcgacggtc aaccaggtca
     1321 agacggtgct ctccagtccg gaagccatca ctgccgtttg caaatcactt gaactgaagg
     1381 gtgtccagat caacgaagac gagatcgtga tgggactaca ccaactcggg gaggcttggg
     1441 agcagctgta cccggccgag cggatcagga tcgtcaaatt gatgattgag cgggttgatc
     1501 tggttgcggg tggactgaag gtgaagtggc acgccctggg ctggaaggaa ctcatcaaag
     1561 agtttgcagc aaagggcatt ggtgcggaat tggtggaaat ggaggccgca tgaacgctga
     1621 cctcgaatcc tttgtgcccc tcacttattc ccggcgtggc gtgcagcggc ttgccaccac
     1681 cagtacagtg gcgcatgaca cgacgttttt ggtcggattg agtcgtgcgt tttactggaa
     1741 aagcctgctc gacaccggcg tcatgaaaag tggctccgaa attgccaaga acgaaggcct
     1801 tcaccactcg gtggtcaacg aactgatgcg cctgacactg ctggcacccg acatcatcac
     1861 gcaattgatg tcgggcacac aaccacggcg gctcaactta atgtggtttc agcggaaccg
     1921 attgcctgtg gactggcagg cccagcgcgc catcatcgac ggcttcaaat aggaatcatc
     1981 atggcaagaa atgacaaggg catggtgact ggtgaaccgg tcacgtacca aataccagcg
     2041 ccagcaggcg gtgtgcagat ggagacgttc attccttgga cgttgatcaa gcgaggtgtc
     2101 cggcggcaag tgatcacgcc aattgatgcg ccggagcagt ttcaccggga agttgtcgtg
     2161 gagcgaagcg cgcgcaaaga ggccaaaggc agcccgctga tccgcgcatt ggggctggcc
     2221 cactactggc agcgtttgct tgatgaaggg aaatatcggt cgctgactga aattgctgct
     2281 gccgagggca tggatcgggg gcaagcgaat cgaattgcgc tgctgactcg actggcaccc
     2341 gaaatcatcc atacatattt atccgggggt caagccaact ttgctttgga gcgtctgaac
     2401 ccatacggca tcaatctcca ttggggcgaa caaatcgatc aactgcagaa ttcaaacttg
     2461 cctagtgcaa cccgtatttg atacctgcag aagtcataac gctaaagcta ctacaatgtt
     2521 gccatgcgtt tatggctgat cgtctttttg gcaattttga tgccgttgca gctctcttgg
     2581 gctgcaacgg gtcgctattg ccagcacgaa agcgacgttt cgagcaaaca cgttggtcat
     2641 cacacacacc agcatcaagt cactgaacgc acagagtcgg gcggcgattt ggcaaaaaca
     2701 atttcggtcg atatggattg cggcacgtgc catgcttgct gctcggtggc tgtccatgaa
     2761 cccgatgtcg tcaaaaaaat tgctctgact ttgatgctcg ctccactgcc cagcgtgcag
     2821 tcaagtcctg gccccgaaga tcgtccagaa cggccacagt gggatgccct ggtttgacca
     2881 gggcatcgat ctacgtttaa gctttaaacg cttcgagaaa tcgatccctg tcaatcgcgt
     2941 cagcattcaa tgctgatttg atc
//
LOCUS       DAOSLL010000601         2902 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_29811_length_2902_cov_4.112048, whole genome shotgun sequence.
ACCESSION   DAOSLL010000601 DAOSLL010000000
VERSION     DAOSLL010000601.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29811_length_2902_cov_4.112048"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(249..962)
                     /locus_tag="PLB25_19625"
     CDS             complement(249..962)
                     /locus_tag="PLB25_19625"
                     /inference="COORDINATES: protein motif:HMM:NF014548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRO family protein"
                     /protein_id="HOE43814.1"
                     /translation="METVQVTPKGSSLSEYPCTPKKPHAAVGRGALQLQTMTMGKMSL
                     DVAMIGDDIWFYAPSLARTLEYSTAVQMLRGVPDSEKGVHLVHTLGGVQEANFLVEPG
                     FYRVLTRSQSPVAEPFKCWFFYEVLPSLRKTGQYKMQQEAKRLGVSFEYTEAQWEWLK
                     MRPGLVDLIPLALGGYNSVEITRMLNYNTKNGITARHQIDKLKELGFLPNVIEPRAKQ
                     LERRILAERAAATPAVALK"
     gene            complement(962..1306)
                     /locus_tag="PLB25_19630"
     CDS             complement(962..1306)
                     /locus_tag="PLB25_19630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43815.1"
                     /translation="MLHPSLSHLENIDPRFLDPNAGFVVMPETTESGLDDASLALEGL
                     SCMLESMACQNIKLSNAGKGTQYVELPPDALGALLRMVSDKIAPARNNPTLSAMRDVR
                     PDLFHPNHQGAA"
     gene            complement(1633..2187)
                     /locus_tag="PLB25_19635"
     CDS             complement(1633..2187)
                     /locus_tag="PLB25_19635"
                     /inference="COORDINATES: protein motif:HMM:NF016927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HOE43816.1"
                     /translation="MTSFTVIVDDKAVQAKLTELSGRLKNMSPVLQTIGVGIVERTQR
                     RFETSKDPAGQPWEPYPPGGFTLTMLAAKLAGKKSYVKKDGSLNAKGERAYAGKKLLI
                     GESTNLKNSFHTAVTGNTLTVSSNMAYAAMHQFGGITSAKSMIPGKTIPARPFLPIHK
                     DGTLYPAEQSEIIKALNEYLMDGL"
     gene            complement(2302..>2902)
                     /locus_tag="PLB25_19640"
     CDS             complement(2302..>2902)
                     /locus_tag="PLB25_19640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43817.1"
                     /translation="KGFGYAPGANVNASLQSLIDDKLIKLDAPIGAAMWESLKSVLLQ
                     ERLVAWQSVFDVTRKTMQAGANAVQVHTVAAATVADLAAHNVVLENAAVWMRDTELMH
                     ALRDDKVLRGAAIPDDVWRDLPLHLEAAVPYLDTQDTALIYFIDLGARLGKVAVRMNY
                     NEKGRFDGVRARIVSNFIQTGGMVDQFNQADARYVLLKK"
BASE COUNT          603 a          759 c          865 g          675 t
ORIGIN      
        1 cattgagggc ttcaagcgct atgtggcgtg ggcggtggtg gccagaaatg ttcatgaagg
       61 gagttgagat aatcgaagag ggaaaggtgc aatatttcgg gttttagttg agttttcgcc
      121 caacttatcg catcgctttt tgaaaatctg tttcgcgtgg ggaaactgtc tgaaagggcc
      181 tgaaatgagg ttaaaaacag tgccctttga ggtttcagac acccggcatt gggctccggg
      241 cgatgtcgct attttagggc gacggcaggc gttgcggcag cgcgctcggc caggatgcgg
      301 cgctcaagtt gcttggcgcg gggttcgatc acgttgggca ggaagcccag ttccttgagc
      361 ttgtcaatct ggtggcgggc ggtgatgcca ttcttggtgt tgtagttcaa catgcgggtg
      421 atttccacgc tgttgtagcc gcccagagcc aacggaatca ggtccaccag gcccgggcgc
      481 atcttgagcc attcccactg cgcctcggtg tactcgaaac tcacgcccag gcgcttggcc
      541 tcctgctgca tcttgtactg gccggttttg cgcagactgg gcagcacttc gtagaaaaac
      601 cagcacttga acggttcagc caccggcgat tggctgcgtg tcaacacccg gtaaaaaccg
      661 ggttcgacca ggaaattggc ctcctggacc ccgccaaggg tgtgcactaa atgcaccccc
      721 ttttctgaat ctgggacacc acgcaacatt tgaacagcgg ttgaatattc cagcgtccgc
      781 gccaaactcg gcgcataaaa ccagatgtcg tcgccgatca tcgccacgtc cagggacatc
      841 ttgcccatcg tcatggtttg cagttgcaat gcaccccgcc ctactgcggc gtgcggcttt
      901 ttcggggtgc aagggtactc acttagtgag gaccctttgg gggtaacttg aacggtttcc
      961 atcatgctgc tccttggtgg ttagggtgaa acaaatcggg gcgaacgtcg cgcatggcgc
     1021 tcagggtggg gttgttgcga gcgggggcga tcttgtcgga caccatgcgc agcagcgcgc
     1081 ccagggcatc gggcggaagt tcaacgtatt gggtgccctt accggcgttg ctcagcttga
     1141 tgttctggca agccatgctt tccaacatgc aggacaggcc ttctagggcc aggctggcat
     1201 cgtctaggcc ggattcggtg gtttctggca tgaccacgaa gcccgcattg gggtcgagga
     1261 aacgcgggtc aatgttttcc aggtgagaga gggacgggtg caacatgaaa tgctcctttg
     1321 tcgagactaa tggcccgctt actagacggg tggaggggtg ttagtaacat gcaacaagac
     1381 atgccgccgt ccttacggaa cggcgcaccc ccccaaaaat cggactcgtg gagcgaattc
     1441 ggggcgtaaa aaagccgcat gactgtcggg tgcggctttt ccgcttgttg aggtgttact
     1501 agcacctgat cgacagttta caccacagga aagactattt cccaacacga aacaacccca
     1561 caaactgcaa cgccgcctgg tcgattgaca cagggcggcg cgatggatga agtctatcag
     1621 gtagcgagag cattacaagc catccatcag gtactcgttg agggctttga tgatttcgga
     1681 ttgttcggcg gggtagaggg tgccgtcctt gtggatgggt aggaaagggc gggcaggaat
     1741 tgttttgccg gggatcatgc tcttggcgct ggtgatgccg ccgaattgat gcatggcggc
     1801 ataggccatg ttgctgctga cggtcagggt gttgccagtc acggcagtgt ggaaggagtt
     1861 tttgagattc gtgctctcac cgatcagcag ttttttaccg gcataggcac gctcgccttt
     1921 ggcgttgaga ctgccgtctt ttttaacgta gcttttttta cctgctagtt ttgctgccaa
     1981 catggtgaga gtgaaaccgc ctggcgggta gggttcccag ggctggccag cggggtcttt
     2041 gctggtctcg aagcggcgct gggtgcgctc gacgatgccg acaccaatgg tctgaagcac
     2101 cggggacatg ttcttgagcc gaccggaaag ctccgtgagt ttggcctgaa cggccttgtc
     2161 atcgacgata acggtgaagc tggtcatggt gaagtttgcg tatttctaca gggtttctat
     2221 aatttgacgt gagcggttgt ttccaatggg aatggttgta ggtcgaaagg catgcatgat
     2281 ccggttcgaa tccggcgccg ctcacttctt tagcagtaca tatctggcat ctgcctgatt
     2341 gaactgatcc accatccccc cggtttgaat gaagtttgaa acgatgcgcg cccgcacgcc
     2401 atcaaagcgg cctttttcgt tgtagttcat tctcacagca acctttccca gccgcgcgcc
     2461 caagtcaatg aaatagatca aagccgtgtc ctgggtgtcg agataaggca ctgcggcctc
     2521 cagatgcagc ggcaaatcac gccatacgtc atcaggaata gcagcaccac gcagcacttt
     2581 gtcatcacgc agggcgtgca tcaactcagt gtcacgcatc catactgccg cgttttccag
     2641 caccacattg tgtgctgcca aatcggccac ggtggccgct gccacggtat gcacctgcac
     2701 ggcattggct ccggcttgca tggttttgcg ggtgacatcg aagacggatt gccaggcaac
     2761 caaacgctcc tgcagcagca cagacttcaa tgactcccac atggccgcgc caatcggggc
     2821 atcaagcttg atcaacttgt catcaatcag cgactgcagc gaagcattga cattggcccc
     2881 aggcgcatag ccaaagcctt tg
//
LOCUS       DAOSLL010000602         2893 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_29932_length_2893_cov_4.707188, whole genome shotgun sequence.
ACCESSION   DAOSLL010000602 DAOSLL010000000
VERSION     DAOSLL010000602.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29932_length_2893_cov_4.707188"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="PLB25_19645"
     CDS             complement(<1..655)
                     /locus_tag="PLB25_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HOE43818.1"
                     /translation="MPGLLSNIDPDGLLEFSVVYTDRALNHMSKRFQGVMLDISGILK
                     EVYHAKSAVLVPGSGTFGMEAVARQFASGKKVLVIRNGWFSFRWTQIFDMGAIPAESI
                     VLKARRTGTDPQSPWIPCPVDEVVASIRANKPAVVFAPHVETSSGMLLPDDYLRAIAD
                     AVHEVGGLMVLDCVASGAIWVNMQDTGVDVLVTAPQKGWSGSPCCAMVCLSPLARTAI
                     "
     gene            complement(1293..1703)
                     /locus_tag="PLB25_19650"
     CDS             complement(1293..1703)
                     /locus_tag="PLB25_19650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009516597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HOE43819.1"
                     /translation="MNYVERDNYGMYAQTTGPGPRLMGADTLMGNDVFNLQDEDLGDI
                     KEIMLDMMYGRVSYAVLSFGGFLGVGEKLFAVPWEALKLDTVNKRFTLDVAKERLANA
                     PGFDKDNWPDMSDQTWANDIHDYYGTKPYSGDMR"
     gene            complement(1775..1984)
                     /locus_tag="PLB25_19655"
     CDS             complement(1775..1984)
                     /locus_tag="PLB25_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005668944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HOE43820.1"
                     /translation="MNKDQVKGQVKDIAGKVQAEAGKLVGSTAQRVKGAKLQVEGKAQ
                     KNLGDAKEVVKESRETMKEAAHPHA"
     gene            2339..>2893
                     /locus_tag="PLB25_19660"
     CDS             2339..>2893
                     /locus_tag="PLB25_19660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmpG family muropeptide MFS transporter"
                     /protein_id="HOE43821.1"
                     /translation="MTDQTPTDAKALPRTWADTLRVYLEPPSLRMLVLGFSAGLPLLL
                     VLGTLSFWLREAGIDRTTIGYLSWVGLAYAFKWVWAPLVDRMPIPLLTHALGRRRSWL
                     LLSQLAVMLGLVAMSFNDPQVALTPVVWGALAVAFGSATQDIALDAFRIESAEVDRQA
                     ALAASYQTGYRLAMIWAGAGVLWIA"
BASE COUNT          540 a          848 c          859 g          646 t
ORIGIN      
        1 caatggccgt gcgggccagg gggctcaggc agaccatggc gcaacaaggc gaaccgctcc
       61 agcctttttg cggtgcggtg accagcacat caaccccggt gtcctgcata ttgacccaga
      121 tcgcgcccga ggccacgcag tccagcacca tcaagccgcc gacttcatgc actgcatcgg
      181 ctatggcgcg caagtagtcg tcgggcagca gcatgcccga cgaggtttcc acgtgcggcg
      241 caaacaccac agcgggcttg ttggcgcgaa tgctggccac cacttcatcg accgggcagg
      301 gaatccaggg cgactgtgga tcagtgccgg tgcgccgggc cttgagcacg atcgactcgg
      361 ccgggatggc acccatgtca aaaatctgcg tccagcgaaa gctgaaccag ccattgcgaa
      421 tcaccagcac ttttttaccg ctggcaaact ggcgcgccac cgcttccatg ccaaaggtgc
      481 cgctgccggg caccagcacc gccgatttgg cgtgatagac ctctttgaga atgccagaga
      541 tgtcgagcat cacgccctga aagcgtttgg acatgtgatt cagggctcgg tcggtgtaaa
      601 caaccgagaa ctcaagcaga ccgtcggggt cgatattgga aagaagtccg ggcatgaaaa
      661 gctcctgtaa ttggggtgcc gatggtggtc agcttagcac gaggccaggt gccggcacca
      721 cgtcgccatg gaacttgaaa ttcgccccgc tgacggatca gcgtcagcgg ttcctggcgg
      781 gtacctatcg caaagtgaat tggtaatgat caacgttttg aaaacgccgc gccaccatga
      841 tgactttggt ttgtaccggt ggcccgccca accgtctcgt tggacagcga ctttccggat
      901 cgccccaatg aaacttggca tcgccttttg gcactcaatg acctcagggt caatggcccg
      961 gtctatcaag gcatcgttca ggaggtgccc gtcgaactga acgggtgtcg gcatcgagcc
     1021 aactgcgccg acccatcttg taccggtctt ctgacccgca accgggctta tgggtgggga
     1081 aaatggtgcg cacgcacttg cgccatatct gggtcacatc cgaagtcgga cacaagcctc
     1141 gcatcctcaa aggcattgca ggcgtcaatt catcgcctga ggtgtttcgc ccaagcaggt
     1201 tccacctcgg cgtgacttga cgcgcatttt ttgaatgaaa cgtgccactg ccactttggg
     1261 cgaggcctgg ttcattcgct gctggtcatc gctcagcgca tgtcgcccga gtagggtttg
     1321 gtgccgtaat agtcgtggat gtcgttggcc caggtctggt ctgacatgtc gggccagttg
     1381 tccttgtcaa aaccgggggc gttggccaag cgttcttttg cgacatccag cgtgaagcgc
     1441 ttgttcaccg tgtcgagttt caaggcctcc caaggcacgg caaacagttt ttcgcctacg
     1501 cccaaaaaac cgccgaaaga cagcactgcg tagctgaccc gaccgtacat catgtcgagc
     1561 atgatttcct tgatgtcgcc aagatcttca tcctgcaggt tgaagacatc gttgcccatc
     1621 agagtgtctg cgcccatcag cctggggcct ggaccggtag tttgggcgta catgccgtag
     1681 ttgtcacgtt ctacatagtt catttgaatt tccttttgaa agagaggggg tggcagggca
     1741 tcgaactgcc ttgccgcagg gccgacgcga ctgatcaggc gtgcgggtgg gcagcttcct
     1801 tcatggtttc gcgggattcc ttgacgacct ctttggcatc gccaaggttt ttttgtgcct
     1861 tgccttcaac ctgcaacttg gcacctttga cgcgctgcgc agtgctgcca accagcttgc
     1921 cagcctcggc ctgcactttg ccggcgatgt ccttgacctg acctttgact tgatccttat
     1981 tcatggcttt tccttttgga gtgagtgagt ggagtcgaac gtgagccgcc aattggctct
     2041 ggggtcagct tagttgcccc ccggttgtgg gtctgtgcgc ttgcatacaa aacgcctttg
     2101 tttttcggtc tggcctgatt cgcaagcaca cagaccaata cccacaggtt acgacccgtg
     2161 cgattgccga gttggattgg tcgtgcgcga gcaagtccgc aggccatctt gcgggcacgg
     2221 taacatcttg ctttagcagg aaactggccg gcgacatgac tgacttgcag gcccagcgct
     2281 gccaactcct gtcttttcca ctggcgatgc gcatgcgcat ggcccagctt tgatacccat
     2341 gaccgatcaa acaccaactg atgccaaagc gctgccacgc acttgggccg acaccctcag
     2401 ggtctatctg gagccccctt cgctgcgcat gctggtactg ggattttcag ccggcctgcc
     2461 gctgctgctg gtgctgggca ccttgagctt ctggctgcgc gaggctggca ttgaccgcac
     2521 caccattggg tacctgagct gggtcgggct ggcctatgcc ttcaagtggg tctgggcacc
     2581 gctggtggac cgcatgccga ttccgctgct gacacatgca ctggggcgca gacgcagttg
     2641 gctgctgctg tcgcaactgg cagtgatgct ggggctggtg gcaatgtcgt tcaacgaccc
     2701 ccaggtagcc ctgaccccgg tcgtctgggg tgcgctggcg gtggcgtttg ggtccgccac
     2761 gcaagacatt gcgctcgatg cctttcgtat tgagtcggca gaggtggatc ggcaagccgc
     2821 gctggctgcg tcatatcaaa ccggctatcg gctggcgatg atctgggccg gtgccggtgt
     2881 gctgtggatt gcc
//
LOCUS       DAOSLL010000603         2892 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_29954_length_2892_cov_3.338033, whole genome shotgun sequence.
ACCESSION   DAOSLL010000603 DAOSLL010000000
VERSION     DAOSLL010000603.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2892)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2892
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29954_length_2892_cov_3.338033"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="PLB25_19665"
     CDS             <1..646
                     /locus_tag="PLB25_19665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013518860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HOE43822.1"
                     /translation="AKLVLLDHHKSAAEKLTGFACRCGVVHFDMGKSGARLAWEFFHP
                     DATLPDLVKYVEDRDIWAWKYPESAAFLAALDMEPFDFVRWAQIAAFTRSELEAFMAR
                     GQAMDEKFAKLAADLAEGAQPVAFNGQQGLMVNAPGVFHSLVGDLLSRKSGTFALMWN
                     AGKGGVIKVGLRSQRGFDCIPLAESMGGGGHPQACGFKLPAERLPDLLAGRLEA"
     gene            761..1576
                     /locus_tag="PLB25_19670"
     CDS             761..1576
                     /locus_tag="PLB25_19670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43823.1"
                     /translation="METSRQSSAASLYREGLPFVVEMARQADLVDVAKLRSASYGKHI
                     PSLGSALQKPEDCDYELGCEVIVARSKFDGTLLGTLRTHTNAFKPLPLQMSLRLPERF
                     ANARMVEATRLCVKGSPNASLVRSALFKALFQYCEAQNVDWMMAAGRRPVDRIYDALL
                     FSDVVESGKFYPMAHAGGVLHRVMSLAPSEARVTWKASEHPLYRFVIETRHDDVDLSA
                     MTSLHFPWTYPKVDTESDSSLAPLSDFTPISRFGDLTTVDIDLSTPLHHRFNA"
     gene            complement(1566..>2892)
                     /locus_tag="PLB25_19675"
     CDS             complement(1566..>2892)
                     /locus_tag="PLB25_19675"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE43824.1"
                     /translation="LGRLARPPSALAYFDLDRFKLINDLYGHAAGDKVLKRVSERMKS
                     AIGPKDMLSRVGGDEFVIAFHDSTVAQATVCCEAIVHLIAAQPYQVGRQRFTLNVSAG
                     LVGTARFENAPLKEIISAADTLCRLAKKRTTQRLVVMESDDSFFLHHKQELEVITYLE
                     RGEVPDGLFLVMQPEISLTQPFDSLNFEILLRMRKSDGGVIPAGIIIEAAEMHGKTAI
                     IDRWVISTAIEWLEINAGALTNTRFVSINVSGGSLNDEAFTDELFEMLAKHPTVLPLI
                     CIEITESVALTDIRNVQRFIDRVRSLGAKVAIDDFGAGYSSFGYLKSLSVDALKLDGS
                     LVKDATRSTPGLAIIEAIGGLVSSLGMKSIGEYAEDLATIKALSNAGIDYAQGYAISK
                     PVMPERILSAQSSADFIEDPDILEFMTGLQSKSSSTMSLFDDIKPEAMH"
BASE COUNT          626 a          740 c          826 g          700 t
ORIGIN      
        1 tgccaagctg gtgctgttgg atcaccacaa gagtgctgcc gagaaactga ccggctttgc
       61 ctgccgttgt ggtgtggtgc actttgacat gggtaaatct ggtgcgcgac tggcctggga
      121 gttttttcat ccggacgcta ccttgccaga cttggttaag tatgtcgaag accgcgatat
      181 ttgggcctgg aaataccccg agagtgccgc gtttctggct gcgctggata tggagccttt
      241 tgattttgtc cggtgggcgc agattgctgc attcacacgt tctgagctgg aagcctttat
      301 ggcccgtggg caagccatgg atgagaagtt tgccaagctg gcagcagacc ttgcagaagg
      361 ggcgcagccc gttgccttca atgggcagca ggggctgatg gtgaacgcac ccggggtctt
      421 tcatagcctg gttggtgatc tgctttccag gaaaagcgga acctttgcgc ttatgtggaa
      481 tgcgggcaaa ggtggagtga tcaaggtagg gctgcgttcg cagcgcgggt ttgactgcat
      541 tcccctggcg gaaagcatgg gtggaggagg gcatccacag gcttgcggat tcaagttgcc
      601 ggcagagcgt ttgccggact tgctcgcagg acggcttgaa gcctagtagt tgcctttctc
      661 ggaaaagcag gaaatggcgg gtatttcact gtcatatgta acatgtaaga ttttgtagct
      721 gaccctacac tggtcacgac tttgccaatc agggggctac atggaaactt cacgtcaatc
      781 ttcagcagcg tcgctgtatc gcgagggcct gccttttgtg gtggaaatgg cccgtcaggc
      841 tgatttggtg gatgtagcca agctgcgctc ggcgtcctat ggcaaacata ttccttcatt
      901 gggatcagcc ctgcagaaac cggaagactg tgactatgaa ctgggctgtg aggtgatcgt
      961 tgcccgctcc aagttcgacg gtactttgct gggaaccctg cgaacgcata caaacgcttt
     1021 caagccattg cccctgcaaa tgtctttgcg actgcctgag cggtttgcca atgcccgtat
     1081 ggtcgaggca acccggcttt gtgttaaagg cagtcccaat gcgtcattgg tgcgaagcgc
     1141 acttttcaag gcgctctttc agtactgcga agcgcagaat gtcgactgga tgatggcggc
     1201 cggacgacgt cctgtagaca gaatctacga tgcactcttg ttctcggacg tagtggagtc
     1261 cgggaagttc tatccgatgg cacatgctgg cggagtactg catcgcgtca tgagcctcgc
     1321 accctccgag gcacgagtga catggaaagc cagtgagcac ccgctgtatc gctttgtgat
     1381 tgaaacgcga cacgatgatg tcgatttgtc cgcgatgacc agcctgcact ttccctggac
     1441 ttacccgaaa gtggatacgg agtccgacag ttctctggcg ccgttgagcg acttcacacc
     1501 cattagtagg tttggcgatc tcacgacggt agatatagac ctctcgaccc ctcttcatca
     1561 cagattcaat gcatagcttc gggtttgatg tcatcgaaaa gtgacatcgt gctggaggac
     1621 ttggattgga gtccagtcat gaactccaat atgtcggggt cttcaatgaa gtccgcgctt
     1681 gattgggctg aaagtattct ttcaggcatc actggcttgc tgatggcata gccttgggcg
     1741 tagtcaattc ctgcatttga gagtgctttg atggttgcca ggtcttccgc gtattcacca
     1801 atggatttca tcccgaggct gctgaccagt ccaccaatag cctcaataat ggcaaggccc
     1861 ggcgtgctgc gtgtggcgtc cttcacaagt gagccatcca gtttcaacgc atccacagag
     1921 aggctcttga gatagccaaa tgatgagtag ccagctccga aatcatcaat ggcaaccttt
     1981 gcgcccagcg agcgcacccg gtcaatgaag cgttgaacgt tgcgtatatc ggtcagtgcc
     2041 accgactcgg taatctcaat acagatcaat ggcaggacgg tcgggtgttt ggcaagcatt
     2101 tcaaacagct cgtctgtgaa tgcttcgtca ttcagggatc cccccgatac gttgatgctg
     2161 acaaatcggg tgtttgtcaa agctccagcg ttgatttcca gccactcgat ggcagtggaa
     2221 atgacccagc gatcaatgat ggcggtcttg ccgtgcattt cagcggcttc gatgatgatt
     2281 cctgccggta tgacaccgcc atcggatttg cgcatccgca gcaagatctc gaaattcagg
     2341 gaatcaaatg gttgcgtcag ggatatttcc ggctgcatga ccagaaagag gccgtccggg
     2401 acttcgccgc gttcgaggta agtaatgact tccagttcct gcttgtggtg cagaaagaaa
     2461 ctgtcatcgg actccatcac gacaagccgc tgtgtcgtgc gttttttcgc caagcggcac
     2521 aaggtgtctg cggcggaaat gatctctttg agaggggcat tctcaaatcg tgcggttccc
     2581 accagacccg ctgacacgtt cagcgtgaaa cgctgccgcc ctacctggta cggctgtgca
     2641 gcgatcagat gcacgatggc ttcgcaacag acagtagctt gtgccacagt ggagtcatga
     2701 aaagcgatca caaattcgtc accacccacc cgagacaaca tatccttcgg accaatggca
     2761 cttttcatgc gctcactcac acgcttgagt accttgtcac cggcagcgtg cccatataga
     2821 tcattgatca gtttgaacct gtcgaggtca aagtacgcca gcgctgatgg ggggcgagcg
     2881 aggcggccta gc
//
LOCUS       DAOSLL010000604         2886 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_30031_length_2886_cov_4.257153, whole genome shotgun sequence.
ACCESSION   DAOSLL010000604 DAOSLL010000000
VERSION     DAOSLL010000604.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30031_length_2886_cov_4.257153"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PLB25_19680"
     CDS             <1..681
                     /locus_tag="PLB25_19680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43825.1"
                     /translation="ILSYSLNALDSFSYTRDGNSLVAPYVSAVWLPVESVNEPAGQDG
                     VAAAGSLPTLKPTGAELRYGRLRLQNAFGSELLALDVPVQAQYYAAGVFKLNAADVCT
                     ALSVPPVIAPRAAGAALDGTAGLYFYTVDAAANGKNKLASSNTTPTLASPLAAGQSKL
                     HFPKPAPSNRGWLDIILQVPPHLLGNWGNCSGQTGAAGLYDDLPCARATFGIFGANSP
                     ILYRRENY"
     gene            756..1028
                     /locus_tag="PLB25_19685"
     CDS             756..1028
                     /locus_tag="PLB25_19685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43826.1"
                     /translation="MNISRVAGSRSFCPSSRIICRLGRKNVRSKDILKRIAVDIARLL
                     LHLQADSAEIIDTECHRMEDRCADLVAQNVRCRWRICPAQGFARHR"
     gene            1231..1461
                     /locus_tag="PLB25_19690"
     CDS             1231..1461
                     /locus_tag="PLB25_19690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43827.1"
                     /translation="MRSQVKHSQLPSCQPGWAQGEIKGQAGLLQRQLARWFATLDAAT
                     QTRLTPATLAQLAYWTDNILDAGAPGIVFKLN"
     gene            1615..2688
                     /locus_tag="PLB25_19695"
     CDS             1615..2688
                     /locus_tag="PLB25_19695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43828.1"
                     /translation="MRWPWHRGRSVNQWVLSWSGQSLTFVHARALADGTFQVLSIGTA
                     QQGAEDHNVFVKRMQGLGMKGAQATIMLRSEQYQLLQINTPAVPPEELRSAARYQVRE
                     MLQTHVDDVTIDVMRVGDGEHKGAGHSFVVAATNQVVKEVLDLAAALGCQVSVIDIQE
                     TAQRNLQTALTRRDGTLDRANAALILDDAQQAVLTISANEELFYTRRFDLPAGFLSGT
                     WGQVVVAQAPVDGFTPVEEYVPDYGGGDAYGSDYSDARPLPAAGVRADDEKSQRLVVE
                     IQRSLDVWDRTWSSLPLSGLRVFAGDRSTELAQWLTQQLGHPVMAMDVTTLFAGFEQA
                     SSTDQALCLPLLGVLLRTEGEAA"
BASE COUNT          568 a          843 c          866 g          609 t
ORIGIN      
        1 atcctgtcgt attcgttgaa tgcgctcgac agtttttcct acacgcgcga cggtaattca
       61 ctggtagccc cctacgtcag cgcggtctgg ttgccggtgg aaagcgtcaa cgaacctgca
      121 ggacaggacg gcgtggctgc tgccggaagc ctgcccacgc tcaagccgac tggcgcagag
      181 ctgcgttatg gtcgtctgcg cctgcaaaac gcttttggat ccgaattgct ggcgctggac
      241 gtgccagttc aggcgcaata ttacgcagcg ggcgtattca aattgaatgc agccgatgtc
      301 tgcaccgcct tgagcgtgcc accggtcatt gcgccccgtg cagccggggc cgcgctcgac
      361 ggcactgccg gtttgtactt ttacacggtg gatgcggctg ccaatggcaa gaacaagctt
      421 gccagcagca acaccacgcc gacgcttgcc agcccgctgg cagcaggcca gtccaagctg
      481 cattttccca agccagcgcc gtcaaaccgt ggctggctcg acatcatttt gcaagtgcca
      541 ccccatttgc ttggcaactg gggcaactgc agcggccaga ccggtgccgc cgggctgtac
      601 gacgacctgc catgcgctcg cgcgaccttt ggcatcttcg gagcgaactc cccgattctt
      661 taccggcggg aaaactactg aatcggcaat tcaatgcctt tttggcctcc agcgcttatg
      721 ctacatgcgc aggcagctat gttaaatata gcaagttgaa catctcacgg gtggccggtt
      781 caaggtcttt ctgtccatca agcaggatca tctgtcgcct tgggcggaaa aacgtccgct
      841 ccaaagacat cctgaaacgc atcgccgtcg acattgcccg cttgctactg cacctgcagg
      901 ccgattcagc cgagatcatc gacaccgaat gccaccgcat ggaagaccgc tgcgccgatc
      961 tggtggcgca aaatgtcagg tgccgatggc gtatttgtcc tgcgcagggg tttgccaggc
     1021 accgctgacg cagtacattg cggacgcaat ggtgctggtg gtccggtgta atttcaagca
     1081 gcgcacaccg cctgaggtga tacatttcaa cgttgaccga cggtagcaac tcaccgctga
     1141 caaagagggg tactttcgca actgcatgcg gacgttggat atcctctcga ccaaccgcga
     1201 tcttggaaac ccatcaaaga ggaagaagca atgcgtagcc aagtcaaaca cagccaattg
     1261 ccttcctgcc aacctggctg ggcgcagggt gagatcaaag gccaggctgg attgcttcag
     1321 cgccaactgg cccgctggtt tgctaccctg gatgctgcca cacagacccg cctgacaccg
     1381 gccacgctgg cgcaactcgc gtattggacc gacaacatcc ttgatgctgg cgcaccgggc
     1441 atcgttttca aactgaactg atcagatgcc agaatttgtg cattttcggc cttcagccca
     1501 gttacgccgt acgtcggtag ctattaaaaa agcatcattt aaactgtttc ttgctttttt
     1561 gatgtgttcc ccgaatttgt tgtgccttaa tctggtgcgg gagttggtct gaccatgcgc
     1621 tggccatggc atcgcggtcg ctcggtcaac cagtgggtgc tgtcctggtc gggtcagtca
     1681 ctgacttttg tccacgccag agccctggcc gatggcactt tccaggtgct gtccatcggc
     1741 acggcgcagc agggtgccga agatcacaac gtgtttgtca agcgcatgca aggcctgggc
     1801 atgaagggcg cacaagccac catcatgctg cgttcggagc aataccagtt gctgcaaatc
     1861 aatacaccgg ctgtgccacc cgaagaactg cgctcggcag cgcgttacca ggtccgtgag
     1921 atgctgcaaa cgcatgtgga tgatgtgacg atcgatgtca tgcgtgtggg tgatggcgag
     1981 cacaaaggcg cggggcattc ttttgttgtg gcggccacca accaggttgt caaagaggtg
     2041 cttgatctgg cggcggcgct aggctgccag gtgtcggtga tcgacatcca ggaaaccgcc
     2101 cagcgcaacc tgcaaaccgc cctgacccgg cgcgacggca cccttgatcg cgccaatgcc
     2161 gcgctgattc tggacgatgc acagcaggcg gtgctgacca tcagtgccaa cgaggagttg
     2221 ttctacaccc gtcggtttga tctgccagcg ggcttcctgt cgggcacctg ggggcaggtg
     2281 gtggtggcac aggcaccggt tgacggcttc acgccagttg aagaatacgt gcctgactat
     2341 ggtggcggtg acgcctatgg cagtgattat tcggatgcca ggccattgcc agcggccggc
     2401 gtccgcgcag atgacgaaaa gtctcagcgg ctggtggtgg agattcagcg ctcgctggac
     2461 gtgtgggacc gtacctggtc aagcctgccg ttgagcggtt tgcgggtgtt tgccggggat
     2521 cgcagcacgg agttggctca atggctaacc cagcaactcg gtcatccggt gatggcaatg
     2581 gatgtgacga cgctgtttgc cgggtttgag caggccagct cgactgacca ggcgctgtgc
     2641 ctgccactgc tgggtgttct gcttcgcacc gagggcgagg cggcctgagc tgcgcggccc
     2701 gagcgattca ccatggcaca acaagtcaac ctttgccttc cggttctgcg ccagcaaaag
     2761 agccgttttg cggcgcagac gctggcgcag gcgctggctg ccttgctggt cgggggcggc
     2821 atactcagtg ccgtctgggt catcaacctc aatcaggcag gtgccagtct gaaagtcacc
     2881 ttggcc
//
LOCUS       DAOSLL010000605         2883 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_30083_length_2883_cov_3.106082, whole genome shotgun sequence.
ACCESSION   DAOSLL010000605 DAOSLL010000000
VERSION     DAOSLL010000605.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30083_length_2883_cov_3.106082"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..1031)
                     /locus_tag="PLB25_19700"
     CDS             complement(36..1031)
                     /locus_tag="PLB25_19700"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007852883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HOE43829.1"
                     /translation="MKIFRGFKHPDIAPACALTIGNFDGVHRGHQAMLSLLRGEAQQR
                     GVPSCVMTFEPHPRDYFAAVTHNPARAPARVGTLRDKLIDLAQCGVDQTVVLPFDARL
                     ADLAPQDFIDRVLRHGLGASYVLVGDDFRFGARRAGDYAMLDSAGLDQGFDVARMNSY
                     EVHGLRVSSSAVRAALQAGNMPGATRLLGHPYRISGHVVHGRKLGRTLGFKTLNQRFA
                     HWKPAASGIFAVLVYGLVSPPLRGVANLGVRPSLDRRDVNGGRVLLETHCLDWPEQLG
                     PEGAYGKIVRVELLHKLHDERKYNSLDALTAGISQDCLDARAYFDAHPVPDSSRL"
     gene            1221..1778
                     /locus_tag="PLB25_19705"
     CDS             1221..1778
                     /locus_tag="PLB25_19705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011809862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HOE43830.1"
                     /translation="MKVLKLSAQGLPQSWISLEQAVIHYAANEVRWEMGNEVAVFHGG
                     HNAVTGQQSIIAVSSIIGTRGVPNINPFELRPGLTNSKLFVRDRNVCAYCGDRFDESD
                     LTREHITPFAQNGMDVWMNVVTACRPCNHRKSHRTPEQALMPLLYAPYVPSLWEDFIL
                     RNRRILADQMEFLMAHLPKSSRLTA"
     gene            1802..2695
                     /locus_tag="PLB25_19710"
     CDS             1802..2695
                     /locus_tag="PLB25_19710"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HOE43831.1"
                     /translation="MNRLNYQTLSSLALLLSQAFLPVAGLSLGGSALAVQASADATPA
                     LMQARLWQPDRSPTGFLVSEKLDGVRAFWDGQTLRFRSGRQIAAPQWFTAALPKLALD
                     GELWLARGQFDRLSGTVRRTDPVDAQWRDIRYMVFDLPGAPGTFADRVTRLAAAVQDA
                     GQPWLQAVVQQRFEGVAALQTRLKQVLAQGGEGLVLHRADALWSPGRSDALLKFKPEP
                     DEDAKVVGHLPGKGRHAGRLGALLLELPDGQRFALGTGFTDLQRETPPAIGAFVTYRY
                     RDRTANGLPRFASFLRVREAE"
BASE COUNT          557 a          876 c          876 g          574 t
ORIGIN      
        1 ggcatgctgg cgcgtacgcc accggcggtg aaggttcaga gtctgctgga gtctggcacc
       61 ggatgcgcgt cgaaataggc acgggcatcc aggcaatcct ggctgatgcc agcagtcaac
      121 gcgtccagcg agttgtattt gcgctcgtcg tgcagcttgt gcagcagctc cacccgcact
      181 attttgccgt aggcaccttc tggcccaagc tgctcgggcc aatcgaggca atgggtttca
      241 agcagcaccc ggccaccatt gacatcgcga cggtcgagtg acgggcgcac gccgaggttg
      301 gcgacaccgc gcagcggcgg actgaccagg ccatacacca gcaccgcaaa aatgccactg
      361 gcagccggtt tccagtgggc aaaacgctgg ttcagcgtct taaagcccag cgttctgccc
      421 agtttgcgcc catgcaccac atggcccgag atacggtagg ggtgaccaag cagcctggtg
      481 gccccgggca tgttgccagc ttgcagcgcc gcccgaaccg ccgaactcga tacccgcagg
      541 ccatgcactt cgtaactgtt catgcgcgcc acgtcaaaac cctggtcgag cccggcgcta
      601 tctagcatgg cgtaatcgcc cgcgcgccgc gcgccaaagc gaaaatcatc accaaccagc
      661 acatagctgg cccccaaccc gtggcgcagc acccggtcga tgaagtcctg tggtgccagg
      721 tcagccagtc gggcgtcaaa cggcaacacc acggtttggt ccacaccgca ttgggccagg
      781 tcgatcagct tgtcccgcag cgtaccgacc cgagccggag cgcgcgccgg attgtgggta
      841 acggcggcaa aatagtcacg cggatgcggc tcaaacgtca tgacgcaact gggcacgccg
      901 cgttgctgtg cttcgccccg gagcagggaa agcatggcct ggtggccccg atgtacaccg
      961 tcaaagttgc cgattgtcaa agcgcaggcc ggggcaatgt cggggtgctt gaagcccctg
     1021 aaaatcttca tcatttgtta ctaacttgat agcattgaga gcaagagatc agagcgcttg
     1081 aagtcaattt gacacataaa aggggtatat tgtctcccag ttgggtgtat cggaaaccga
     1141 gttggcccgt cttgtgcatc tccaggctgt ttgccggatt tgggggccga tccgattgcg
     1201 cctttttcag gagtcatgtt ttgaaagtct tgaagctgtc agcccagggg ttgccccaat
     1261 cgtggatatc tcttgagcag gcggtgattc actatgctgc gaacgaagtg cgctgggaaa
     1321 tgggcaacga ggtagcggtg ttccacggcg gccacaacgc cgtcaccggc cagcagtcca
     1381 tcatcgcagt gtcgagcatc atcggcacca ggggggtgcc caacatcaac ccgtttgaac
     1441 tcaggcccgg cctgaccaac agcaagctgt ttgtgcgcga ccgaaatgtc tgcgcctatt
     1501 gcggtgaccg gtttgacgaa tcagacctga cccgtgagca catcacgccg tttgcgcaaa
     1561 acggcatgga tgtctggatg aatgtggtca ccgcgtgccg cccttgcaat caccgcaaaa
     1621 gccatcgcac gccagaacag gccctgatgc cgctgctgta cgcaccctat gtgcccagcc
     1681 tgtgggaaga cttcattttg cgcaaccgac gcattctggc agaccagatg gagttcttga
     1741 tggcccattt gcccaagtcg tcacgactga cggcgtaagc caccagtttt cgggagttaa
     1801 ggtgaatcga ctcaactatc aaaccttgag tagcctggca ttgttgctga gtcaggcttt
     1861 tttgcccgtt gcgggcctgt cgcttggcgg gtctgccctg gccgtccagg ccagcgccga
     1921 tgccacgccg gcgctgatgc aggccaggct ctggcagcca gaccgcagtc cgactggttt
     1981 tctggtcagc gaaaaacttg acggcgtgcg cgccttttgg gacggccaga ccctgcgttt
     2041 tcgcagtggc cgacaaattg ccgcaccgca atggtttacc gccgccctgc caaagctggc
     2101 actcgacggc gagttgtggc tcgcgcgtgg tcaatttgac cgcttgtcgg gcacggtgcg
     2161 gcgcactgat ccggttgatg cgcaatggcg ggacatacgc tacatggtgt ttgatttgcc
     2221 cggtgctccc ggcaccttcg ccgaccgggt aacgcgtctg gcggcggctg tgcaagatgc
     2281 cgggcagccc tggctgcaag ccgtggtgca gcagcgcttt gagggcgttg cggcactgca
     2341 aacacggctc aaacaagtgc tggcccaggg cggcgaagga ctggtgttgc accgcgccga
     2401 tgccttgtgg tcaccggggc gcagtgacgc gctgctgaag ttcaagcccg agccggatga
     2461 agatgccaaa gtggttggcc atttgcctgg caagggccgc cacgctggtc ggcttggtgc
     2521 cttgttgctg gaactgcccg acggccagcg ctttgcgctg ggaaccggct tcaccgacct
     2581 gcagcgcgaa acaccgccag cgatcggtgc tttcgtgacc taccgctacc gcgaccgaac
     2641 cgccaacggc ttgccgcgct ttgcctcatt tttgcgcgtg cgtgaggccg aatagcactg
     2701 gccgatcagg ccgcgacatc gagcgtgccg ctgctgttgg acgacacacc tgaggaaatc
     2761 tgctcgtaga ccagcttggc cagtttcagg attgcatcgc tggctgtcga gcttgccttg
     2821 ctgacacttt cactgacgct ttcactgaca ctttcactgg cactgacaaa ttctttcagg
     2881 gca
//
LOCUS       DAOSLL010000606         2882 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_30094_length_2882_cov_3.478599, whole genome shotgun sequence.
ACCESSION   DAOSLL010000606 DAOSLL010000000
VERSION     DAOSLL010000606.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2882)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30094_length_2882_cov_3.478599"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="PLB25_19715"
     CDS             <1..1345
                     /locus_tag="PLB25_19715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010464591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HOE43832.1"
                     /translation="GVGAETLDTVVLPDQGVNANISLPQFDVDAWAAVLAQASAAPIA
                     GAVVAEGLGLGYLPSSVAARTAQLTFGERQYSHVVIGAGRDGLLWRANVDATELNGYL
                     EYRQASDASTASGAGRVYARLARLTVAPSAASEVEALLDAQPASIPALDIVVDDFELR
                     GKRFGRLEVEAINRAVLAQPGAPGAREWRLNKFNLIVPEATLTASGNWARINAQAGQP
                     VAASSIAAAERRRTVLNFKLDIADGGKLLGRFGMPDVVRLASGRMEGQIAWMGSPLKL
                     DYPTLGGAFTVNVASGQFLKAEPGIAKLLGVLSLQALPRRLSLDFRDVFSEGFSFDFL
                     RGDVTVDKGLARTNNLQMKGVNAAVLMEGQADIARETQDLKVVVVPEINAGTASLIAT
                     VINPAIGLGSFLAQLFLRRPLIESATQEFRVDGSWADPRVTKVTHVAPTTKESSP"
     gene            1342..2175
                     /locus_tag="PLB25_19720"
     CDS             1342..2175
                     /locus_tag="PLB25_19720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003060140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HOE43833.1"
                     /translation="MKVAAIQMVSGSDVRANLQQARLLLEEAASTGAELAVLPEYFCL
                     MGQHDADKLAIQEPFGHGPLQQFLSDTAKDLRLWLVGGTIPLAGASADALSSSGRVFN
                     SSLAFSPDGACVARYDKIHLFRFDNGLESYDESRVLMAGDHPVTFDVNSRDGTLWRVG
                     MSVCYDVRFPELYRAYASVGAHLLLMPSAFTYTTGQDHWEVLLRARAIENLAFVAAAA
                     QGGLHDNQRRTWGQAMLVDPWGTVLAQRDQGRGIAVSELDPAVLASCRSRLPALQHRV
                     L"
     gene            2181..>2882
                     /locus_tag="PLB25_19725"
     CDS             2181..>2882
                     /locus_tag="PLB25_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011807096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HOE43834.1"
                     /translation="MGPSLRWLLLVGLFILAACSSAPMQRVPAPVSEAQPSVAATDAI
                     TTPADIATPEPGLLRGKSRWVPVAWSELPGFEGDALFEAWNAWLKSCERPGPVFAYLC
                     PEVRRLSIGGDAAQRQWLLDRLQPYRVEPLQGGLTPGLLTAYFEPEFEARRTPGGGFV
                     VPLYMPPVGLNAGKPWFSRKDIETLPQAQAALRGREIVYLSDPVQALVIQIQGSGRVR
                     VTEPDGGKRLVRLAYA"
BASE COUNT          479 a          841 c          963 g          599 t
ORIGIN      
        1 tggggtggga gcagagacgc ttgacaccgt tgtcttgccc gatcaggggg tcaacgccaa
       61 tatcagtctg ccgcagttcg atgtggacgc ctgggccgcg gtgctggctc aggcaagcgc
      121 ggcaccgatt gcaggcgctg ttgttgcaga agggctgggc ctgggctact tgccaagttc
      181 cgtggctgcg cgcacggcgc agttgacctt tggtgagcgg caatacagcc acgtggtgat
      241 tggggccggg cgcgacggtt tgctctggcg tgccaatgtg gatgccaccg agctcaatgg
      301 ctatctggag taccgacagg catcggatgc ctcaacggcc agtggtgccg gacgggtgta
      361 tgccaggctg gcgcgcctga ctgtggcgcc cagcgctgcc agtgaggtcg aggccctgtt
      421 ggacgcgcag ccggccagta ttcctgcact ggacatcgtg gtggacgatt tcgagttgcg
      481 tggcaaacgt tttggccgac tggaagtaga ggccatcaat cgggcggtgc tggcgcagcc
      541 cggtgcaccg ggcgcacgcg aatggcggtt gaacaagttc aacctgatcg tgcccgaggc
      601 cacattgaca gccagcggaa attgggcccg gattaatgcc caagcggggc agcctgtggc
      661 cgcgtcgtcc atcgctgctg ccgagcgtcg gcgcaccgtg ctcaatttca agttggacat
      721 tgctgacggc ggcaagttac tggggcgctt tggcatgccg gatgtggtgc gtctggccag
      781 tggccgcatg gaaggccaga ttgcctggat gggctcaccg ctcaaactgg attacccgac
      841 gctgggtggg gcatttacgg tcaatgttgc ttcaggacaa tttctcaagg cggagcccgg
      901 cattgccaaa ctgctcggtg tgctgagtct gcaagccttg ccgcgacggc tttcgcttga
      961 ttttcgcgat gtgttcagcg agggtttctc gtttgacttt ctgcggggtg acgtgacggt
     1021 tgataagggc ctggccagga ccaacaacct gcaaatgaaa ggtgtgaatg cggcagtcct
     1081 gatggagggc caggccgaca tcgcccgtga gacccaggac ctgaaagtgg tggtcgtgcc
     1141 tgaaatcaac gcaggtacgg cctccctgat tgcgaccgtc atcaaccctg ccatcggcct
     1201 gggctctttc ctggcgcaac tttttctgcg tcgcccgctg atcgaatccg caacccagga
     1261 gtttcgggtc gatggcagtt gggctgaccc ccgagtcacc aaagttaccc atgtcgcccc
     1321 cactaccaag gagtccagcc catgaaagtc gcagccatcc agatggtgtc cgggtcagat
     1381 gttcgcgcca acctgcaaca agcccggctg ttgctggagg aggcggcaag taccggtgcc
     1441 gagctggcgg ttttgccaga gtatttttgc ctgatggggc agcacgacgc ggacaagttg
     1501 gcgatccagg agccgtttgg ccacgggccg ctgcagcagt tcctgtcgga tacggcgaag
     1561 gacctgcgct tgtggctggt gggtggcacc atcccgcttg ctggtgccag cgcggatgcg
     1621 ctgtcaagtt ctggtcgggt gttcaacagc tcgctggcgt tttcacccga cggtgcctgt
     1681 gtcgcgcgct atgacaagat tcacctgttt cgatttgaca acggccttga atcttatgat
     1741 gagtcccgcg tgttgatggc gggcgatcac cccgtgacct ttgacgtgaa ctcgcgcgac
     1801 ggaacgctct ggcgtgtggg catgagcgtg tgctacgacg tgcgttttcc cgagttgtac
     1861 cgcgcctacg ccagcgtagg cgcgcatctg ctgctgatgc cgagtgcttt cacctacacc
     1921 actgggcaag accattggga ggtgctgctg cgcgcccgcg caattgagaa tctggcattt
     1981 gtcgccgccg ccgcgcaggg cggtttgcat gacaaccaac gtcgcacctg gggccaggcg
     2041 atgctggtcg acccctgggg cactgtgctg gcccagcgag atcagggccg cggtatcgcc
     2101 gtgtccgaac ttgatcctgc tgtgctggcc agttgtcgca gccgcctgcc tgccctgcag
     2161 catcgcgtat tgtgaagaat atgggaccct ctcttcgctg gctgctgctg gtcggcctgt
     2221 ttattctggc ggcctgcagc agcgcaccca tgcagcgggt gccagcaccg gtcagtgagg
     2281 cccaaccgtc ggtcgccgcg acagacgcca tcacaacgcc agcggacatc gcgacgcccg
     2341 agcctggcct gttgcgtggc aaaagccgat gggttccggt tgcctggagc gaactgcccg
     2401 gttttgaagg cgatgcacta tttgaagcct ggaacgcctg gctgaaaagc tgtgagcgtc
     2461 cggggccggt atttgcttac ctgtgcccgg aggtccgccg cctgagcatc ggcggtgatg
     2521 ccgcccagcg tcagtggttg cttgaccgcc ttcagcctta ccgtgtcgag ccgcttcaag
     2581 ggggcctcac acctgggctg ctgacggcct actttgagcc ggaatttgaa gcccgacgca
     2641 cgccaggtgg tgggttcgtg gtgccgctgt acatgccgcc cgttgggctc aacgctggca
     2701 agccctggtt ttcacgcaaa gacattgaga cgctgccgca ggcgcaggcc gccttgcgcg
     2761 gtcgcgagat cgtgtacctg tctgacccgg tgcaagcgct ggtgattcag attcagggct
     2821 cgggtcgggt gcgtgtgacc gaaccggatg gcggcaaacg cctggtgcgt ctggcctacg
     2881 cg
//
LOCUS       DAOSLL010000607         2881 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_30106_length_2881_cov_4.293347, whole genome shotgun sequence.
ACCESSION   DAOSLL010000607 DAOSLL010000000
VERSION     DAOSLL010000607.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30106_length_2881_cov_4.293347"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="PLB25_19730"
     CDS             <1..178
                     /locus_tag="PLB25_19730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HOE43835.1"
                     /translation="QITPPVGFNLFVLQGMTGKQLPYIARVSMPMFFLMVAAVLLIYF
                     VPGIVTWLPQQMKS"
     gene            244..2520
                     /locus_tag="PLB25_19735"
     CDS             244..2520
                     /locus_tag="PLB25_19735"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HOE43836.1"
                     /translation="MDSLNDTLAAKLAAVQRPGDYFVSGTVEAYLPQLEVESVGRVAL
                     PLLPQQAQQLIAVATQAPYGRGSETLIDTAVRKTRQINADQVKLSGRHWPQTLQEILD
                     RVVAGLSVSKPVHAELYKLLVYEDGDFFVSHRDTEKAPGMFATLVIVLPSVYTGGELI
                     IRHNKREVELDLCCEDASELVAFAAFYADCVHEVKPVTSGCRLTLIYNLVRPGSGQLP
                     QAPDYGAEQDDVASLLQQWVAAQGKTEPTAEKLIYPLEHAYTAAELRFDQLKGADAAI
                     APLLLAATEKAECELHLALLTIEESGSAEYADVQPRRGWHHSAYAEDSYEVGEVFERR
                     AHLSEWRTPTGRNAGLRELPFHEAEICPLGALGDMEEAELEFHEATGNEGVSFDRSYC
                     RAALVIWLGAQKLAMISQAGLRQSLPYLSELVARLATDDAPALRQQAVQLAALMLSTW
                     PRRNQYHSGRDPNQASMLELLDKLADTALLERFLIEVAAQGNYSQADNPALTAACRLL
                     PTARAVDLLVLIIMANKDVALAGCCGLLHAGVGLAGEVALLAKLLPVATSLCQALPDG
                     TAKPAAHAGAQAPITDSGMMVDLLRALGRLDASLGSDLAASTSRRLLATPEIYPLDGL
                     IVPVALQMVGDVGAELLHQASLTHLKDRIAEPLQAPVDWRRDSSIACRCKDCAALSQF
                     LADPSLPRWLFKAAEPARRHVQASVTHNQVDVDCSTDNSGRPYKLVCAKNQANYQRRV
                     TQRGRDIADLALLQQAPG"
BASE COUNT          582 a          839 c          870 g          590 t
ORIGIN      
        1 ccagatcacc ccaccggtcg gcttcaacct gttcgtgctg cagggcatga ccggcaagca
       61 attgccttac attgcccgcg tttccatgcc aatgttcttt ctgatggtgg ccgcagtgtt
      121 gctgatctat tttgtgccgg gcatcgtcac ctggctgccg cagcagatga agtcatagca
      181 gctacagcaa gcatggcaac ccggccaatc tgaactgaaa tcaacgcact cctggaattc
      241 acgatggact cactgaacga cacgcttgcc gccaaactcg ccgcagtgca gcgtcctggc
      301 gattattttg tttcgggcac ggtggaagcc tatttaccgc aactcgaagt tgaatcagtc
      361 gggcgggttg ctttgccgct gctgccgcag caagcgcagc aactgattgc ggtggcaacc
      421 caggcgccct acggacgtgg cagtgagacg ctgatcgaca cggcggtacg aaagacccgg
      481 cagatcaatg ccgatcaggt caaactcagt ggtcgccatt ggccgcaaac gctgcaggaa
      541 attcttgacc gggttgtcgc aggcctgagt gtcagcaagc cggtgcacgc cgagctgtac
      601 aagctgctgg tgtacgaaga cggcgacttc tttgtcagcc accgcgatac cgaaaaggcc
      661 cccggcatgt ttgccacgct ggtgatcgtg ctgccgtcgg tctataccgg gggcgaactg
      721 atcattcgcc acaacaagcg cgaggtggag ctggacttgt gctgcgagga tgcgtccgag
      781 ctggtggcct ttgcggcgtt ttacgccgac tgcgtgcatg aggtcaagcc ggtcacctct
      841 ggctgtcggc tgacgctgat ctacaacctg gtccgcccag gctcgggtca actgccgcag
      901 gcacccgact atggggccga gcaagacgat gttgcctcac tgttgcagca atgggtcgcg
      961 gcacaaggca agactgagcc gacggcggaa aaactgatct atccgttgga gcatgcctac
     1021 acggcggccg agcttaggtt tgaccagttg aaaggcgctg atgccgccat tgcgccgctg
     1081 cttttggctg ccaccgaaaa ggctgaatgc gaacttcatt tggctttgtt gaccatagag
     1141 gaaagcggca gcgccgaata tgctgatgtc cagccacgtc gtggctggca tcacagcgcg
     1201 tatgccgaag acagctacga agttggcgag gtttttgagc gacgcgctca cctgtccgaa
     1261 tggcgcacgc caactggccg caacgctggc ttgcgtgagc tcccgtttca cgaagccgaa
     1321 atttgcccgc tcggtgcatt gggtgatatg gaagaggctg aactggagtt tcacgaggcc
     1381 accggtaacg aaggggtctc ttttgatcgc agctattgcc gcgcagcgct ggtcatttgg
     1441 ctgggggcgc aaaaactggc catgatcagc caggccggac taaggcagag ccttccctat
     1501 ctgagtgagc tcgtcgcgcg cctggccacg gacgacgcac cggcgctgcg gcagcaggca
     1561 gtgcagctcg ctgcattgat gctgagcacc tggccgcgcc gcaatcaata ccactcgggg
     1621 cgagatccga accaggcaag catgcttgaa ctgctggaca agctggcaga tactgccttg
     1681 ctggagcgct ttctgattga agtggcagcc caaggcaact acagccaggc cgacaacccg
     1741 gcgttgaccg ccgcctgtcg cttgctgccg acggcgcggg cggttgactt gctggtgctg
     1801 atcatcatgg cgaacaagga tgtggcgctt gccgggtgtt gtggtcttct gcacgccggt
     1861 gttggcctgg cgggtgaagt cgcactgctg gcaaagctgc taccggtcgc aaccagcctt
     1921 tgtcaggcgc tgcccgacgg tacggccaaa ccggcggcac acgcgggtgc acaagcgccg
     1981 atcaccgatt ccgggatgat ggttgatttg ctgcgggcgc ttggccgact tgatgcctcc
     2041 cttggcagcg atttggccgc aagcaccagc cgacgcttgc tggcaacgcc agagatttac
     2101 ccgctggacg gtctgattgt tccggttgcg ctgcaaatgg tgggtgatgt cggcgcagaa
     2161 ctgctccatc aggccagcct gactcacctg aaagaccgca tcgccgaacc cctgcaggca
     2221 ccggtcgatt ggcgtcggga cagctcgatt gcctgccgct gcaaggattg tgctgctctg
     2281 agtcaatttc tggccgaccc gtcgctgcca cgctggctgt tcaaagcggc agaaccagcc
     2341 cgtcgacatg tgcaggcatc cgtcacccac aaccaggtcg atgtggattg cagcaccgat
     2401 aacagtggtc gcccctacaa gctggtgtgt gccaagaatc aggccaacta ccagcgccgc
     2461 gtgacacaac gtgggcgtga catcgctgac ttggcactcc tgcagcaggc acctggctga
     2521 cgcatcgaac cggcgcaatg ccatttcacc tgagctttgg cgaccgctgc aacctgaagc
     2581 cgccgcagca attagatgcg attgccgatg atcctggatg ccacagttga ccgcttgttt
     2641 ttatctggaa ttgctgatgc gcatttactt cttcagcgtc gaccggagtc accaacgggt
     2701 gaactccatc gcagttagaa aagtaaatgc acatcaggac ttagagatga aaacaagcgg
     2761 tcaactgaat ttaggcgcta tgcgcgtcaa acttaccaac ctggacagtg cgtagtttcc
     2821 cctttatgtt caggccgcca ttggctgcgc atcagagggt gggtcatggc ggcgatattt
     2881 g
//
LOCUS       DAOSLL010000608         2869 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_30257_length_2869_cov_4.159559, whole genome shotgun sequence.
ACCESSION   DAOSLL010000608 DAOSLL010000000
VERSION     DAOSLL010000608.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30257_length_2869_cov_4.159559"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(479..1216)
                     /locus_tag="PLB25_19740"
     CDS             complement(479..1216)
                     /locus_tag="PLB25_19740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HOE43837.1"
                     /translation="MRIAIISDIHGNLPALQAVTKDIGRRGVDSIVNLGDSLSGPLLP
                     LETAQFLMAQDWVQLAGNHERQLLTLSPGQRGLSDEFAHSQLTTKEFEWMASLKPSVK
                     YSSEVLLCHGTPGNDIAYFLETVEPNQLRAATLSELDKRLGSVDAELIACGHTHVPRS
                     MRASTGQLVVNPGSVGLQAYDDSRPYPHAIETGSPDARYAIVERLAGAWVSHLISVPY
                     HHQSMVKLALARQRPDWACALATGRMT"
     gene            1746..>2869
                     /locus_tag="PLB25_19745"
     CDS             1746..>2869
                     /locus_tag="PLB25_19745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43838.1"
                     /translation="MELSIVMDASTDSVRGKGRKAWILLGMLMLLVAASLGGHSYVEY
                     YDTIDRELKAFEEHVRLADVQITSALQRTDMITQSVVTDELSSHKLPATAAHQHKLDL
                     LQQFPEVDALFVLDSMGRVVDAASIHMPEVLPRLKSLDASQQEYFSVHRNARVEDTRR
                     NWLSRPFKETGDRYIVALSRAIRGNDGVLQGVVVIFTSPKFFDNVLSELLRNGAIDAA
                     AVHNRQGDIIYRLPNPSAYVGKNIGSAESFKKYLASTEKMTRYVGVVATDGVKRVLVF
                     GKVGDTTLDVAVSVRTDSLMADWKNRTLWKLLIFLAIALLMTLLVWQTQRRRVFRDER
                     ERAERRFRAYFERSMVGMATLGPANELLDINQTLCEMLGFS"
BASE COUNT          639 a          726 c          825 g          679 t
ORIGIN      
        1 cgctggtcgt ctgggtcaat cctgcaaccc aagcaaggtg ccaagctgat tgttgtcgat
       61 gcacggcatg gcgactgggt catgcacgtg cggttttggg gcgaaatcac ccgcgacgat
      121 tgctgatcgt gtgcgaacgc tgttggcgcg cgcctttcgc cccaaagccc ggtcacatga
      181 cccagccacc acgcctcagg gtggtttgaa agggcataag cgcagagtcg gggtgcaggt
      241 tgtgtgtggg tatgcagttg agccccgtca gcggcaggtt acggtaagtc cagttcagcg
      301 aggtggcacc aacccattcg ttagcctgcc ttttgctggt tggcaataga tgccttgatg
      361 tcatccgcgc cgcttggtgc cagtcagtca aggtctgaat tgaccagttg gcgcggttcc
      421 atcgcagggc aaatttgttc tttcgcgcag tgcttgccaa atgatgggtt tgcggccttc
      481 atgtcatacg tcccgtcgcc agcgcacatg cccagtctgg cctttgccgc gcaagcgcaa
      541 gtttcaccat tgattggtga tggtacggca ccgaaatgag atgagaaacc cacgcgccag
      601 ccagcctctc cacaatggcg tagcgtgcat cgggcgagcc ggtttcgatg gcatgtgggt
      661 agggtcgaga atcgtcataa gcttgcagtc caacacttcc cggattgacc accaactgac
      721 ctgttgaggc gcgcattgat cgtggtacgt gagtgtgtcc gcatgcaatc agctcggcat
      781 caacactgcc aagacgtttg tcaagttctg acaaggtggc agcgcgaagc tgatttggct
      841 cgactgtttc aagaaaatac gcaatgtcgt tgccaggagt tccatgacaa agcaatacct
      901 ccgagctgta tttgacactt ggcttgagcg atgccatcca ctcaaattcc tttgtggtga
      961 gctgcgagtg cgcgaattcg tcggacagtc cccgctgacc agggcttagc gtgagaagtt
     1021 gtcgttcatg atttcctgcc agttgcaccc agtcttgcgc catcaaaaat tgtgctgttt
     1081 caagtggtag cagaggacca gacaggctat cgcccaggtt gacgatcgaa tcaacgccac
     1141 gacggccaat gtccttggtg actgcctgaa gcgctggcag gtttccatgt atgtctgaaa
     1201 tgatggcgat acgcatatgg caactttcgg aaggctaact tcgggggtgg acggcgtatt
     1261 ttggcatcag ccagtatgac cggccggtcc cggaattgga cgagtggatc agacagcgta
     1321 tgcgtatgtg ctactggaaa cagtggcgtt ggccgcgcac gaagatcaag cgtttgctgg
     1381 cgtttggcgt gagcctgaaa tcggcactgc tgcctgatac agggctcaca aggaacagat
     1441 attttgttaa caatcaaaaa tatagctagt aacgcagtct gcataagggc tggaagccga
     1501 tttggtagat gaaaccccgg cagcttgcgc cgtcggccta gcccatgttc ctttgagaac
     1561 gcctgccccg cgtgcggggt tgggatgctg agtagttacg aaaaatctaa gacttcagga
     1621 gggaaacatt cagatttgga tgactgacaa gtcctgcgcc cttgtgtaat atcagtcgcg
     1681 ctgatgcatg gaaatcaact cgatgacggc atagcgcatc aggcggcaaa tggttgtcac
     1741 gatttatgga actctcaata gtgatggacg cctcgacaga cagtgtgcgg ggtaaaggcc
     1801 gtaaggcctg gatactgctg ggcatgctca tgctgcttgt cgctgcatcc cttggcgggc
     1861 atagctatgt cgagtactac gacactatcg accgggaact caaagccttt gaggaacatg
     1921 ttcgcctcgc agatgtgcag atcacctctg ccctgcagcg cacggacatg atcacgcaaa
     1981 gtgtggtgac tgacgaactg tccagccata agttgcccgc cacagcagcc caccagcata
     2041 agctggacct gttgcaacag ttccccgaag tagacgcctt gtttgttctt gattccatgg
     2101 ggcgcgttgt ggatgccgca agcatccata tgccagaagt cctgcccagg cttaagagcc
     2161 tcgacgcatc ccaacaagaa tacttttcag tccatcgaaa tgcccgtgtc gaagacaccc
     2221 gacgaaactg gctgtctcgt ccctttaaag agacggggga tcgttacatt gttgccctgt
     2281 cgagggcaat tcgaggcaat gacggtgtgc ttcagggggt ggtcgttatc ttcacctctc
     2341 ccaagttttt cgacaacgtt ctgagcgagc ttctacgcaa tggggcaata gatgctgccg
     2401 ctgttcacaa ccggcaggga gacatcatct atcggcttcc aaaccccagt gcttatgttg
     2461 gcaagaacat tggaagcgct gagtccttca aaaagtattt ggcttcaaca gaaaagatga
     2521 cccgctatgt gggtgttgtc gctaccgatg gggtgaagcg tgtcctggtg tttggaaagg
     2581 tcggagatac caccttggac gtcgcggtct cagtgagaac ggactcactc atggcggact
     2641 ggaaaaacag aacgctgtgg aagttgttga tatttctggc tattgccttg ctgatgacct
     2701 tgcttgtctg gcagacacaa cggcgccggg ttttccgaga tgaacgagaa agagccgaga
     2761 ggcgttttcg tgcctatttt gagcgttcaa tggtcggcat ggcgaccctt gggcctgcaa
     2821 atgaattgct ggatatcaac cagactctgt gcgaaatgct gggtttttc
//
LOCUS       DAOSLL010000609         2861 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_30379_length_2861_cov_4.191019, whole genome shotgun sequence.
ACCESSION   DAOSLL010000609 DAOSLL010000000
VERSION     DAOSLL010000609.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30379_length_2861_cov_4.191019"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(181..1395)
                     /locus_tag="PLB25_19750"
     CDS             complement(181..1395)
                     /locus_tag="PLB25_19750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HOE43839.1"
                     /translation="MTLMVHGRAFAHIAAFHPELTAFRRDLHAHPELGFEEVYTAARV
                     KQALELCRVDEIHTGIGKTGLVAVIKGQRNSSAKMIGLRADMDALPMTEHNDFAWKSA
                     QPGLMHGCGHDGHTAMLVGAARYLAETRRFDGTAVLIFQPGEEGYAGAQAMIEDSLFE
                     RFPVQSVFGMHNWPAMRPGTIGLNSGPMMAAADHLCIEITGLGGHGGHAYQSVDPVLV
                     AAHIITAVQSIVSRNVKAVDSAVISLCAMQAGDLNAMSVIPGRATLTGTVRSFSTEVQ
                     ALIERRLRELCSGIAQAFGASAALTYTRMYPATINAAAQAEFAGDVAQSLVGAANVVR
                     DLEPSMGAEDFSFMLRVKPGAYLRLGQGAPSGQGSCHLHNNRYDFNDAVLPLGAALHA
                     SLAEQAMPLESV"
     gene            1594..2613
                     /gene="galE"
                     /locus_tag="PLB25_19755"
     CDS             1594..2613
                     /gene="galE"
                     /locus_tag="PLB25_19755"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HOE43840.1"
                     /translation="MMIFVTGGAGYIGSHTCVELLNAGHQVTVFDNFSNSQPEALKRV
                     ERITGSRLQLITGDIRDQTALEAALSASQPQAVIHFAGLKAVGESVAQPLKYYDNNVA
                     GTLKLLQAMQACDIRTLVFSSSATVYGDPQRLPLTEDHPLSATNPYGQTKLVIENMLR
                     DLSASDSSWRIGILRYFNPVGAHASGLIGEDPQGLPNNLLPFVAQVAVGRRAFLNVWG
                     NDYATPDGTGVRDYIHVVDLALGHLAALALLRGHATCFEVNLGTGTGYSVLDMVRAFE
                     LASGKPVPYTISPRRPGDVAACYADPAFARELLGWHAQRDLQTMCLDAWRWQSGNPNG
                     YSAAS"
     gene            2662..>2861
                     /locus_tag="PLB25_19760"
     CDS             2662..>2861
                     /locus_tag="PLB25_19760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HOE43841.1"
                     /translation="MTKGMILAAGQGTRIRPLTKDMPKPMIPILGKPVMEYLIEHLAS
                     YGITEIMVNVAFNHHKIEQYFG"
BASE COUNT          616 a          871 c          814 g          560 t
ORIGIN      
        1 ttcagtgcac ccctatatag atcaacacgt tacaaattta gctggggaac tcgggggtcc
       61 gaagtcgcct gcacaactgc cgctgtttcg acaacagcgc tggccacctt tcaaagtttg
      121 gctttggatt gccctctagc ccttatggaa tatgcgctga cagctatgaa tagcagagta
      181 ctaaacggac tccagtggca ttgcctgctc ggccagactg gcgtgcaggg cggcacccag
      241 tggcaacacg gcatcgttga agtcgtaccg gttgttgtgc agatggcaac tgccctgccc
      301 gcttggtgcc ccctgcccca gtcgcaggta agcgccgggc ttgacgcgca gcatgaaaga
      361 aaaatcttcg gcccccatgc ttggctccag atcacgcacc acgttggccg cgccaaccag
      421 gctttgcgcc acatcaccgg caaactcggc ttgcgcggcc gcgttgatgg tggccgggta
      481 catgcgggtg taggtcagcg ccgcgctggc accaaaagcc tgcgcgatgc cgctgcacaa
      541 ctccctcagc cgacgctcaa tgagcgcctg cacttctgtg ctgaagctgc gcacggtgcc
      601 ggtcagcgtg gcacggcccg ggatgacgct catggcgttg aggtcgccgg cctgcatggc
      661 gcacaggctg atcaccgcgc tgtccaccgc cttgacgttg cgcgacacga tgctttgcac
      721 ggcagtgatg atgtgagcgg cgaccagcac cgggtcaacg ctttggtagg catggccgcc
      781 atgaccaccc aggccggtga tctcgatgca aagatggtca gcagcagcca tcattggtcc
      841 ggaattcagg ccaatggtgc cggggcgcat ggctggccag ttgtgcatgc caaaaacgct
      901 ctgaaccgga aaccgctcaa acaagctgtc ctcaatcatc gcctgcgcac cggcataacc
      961 ctcttcgcct ggttgaaaaa tcagcactgc agtgccgtca aagcgacggg tctcggccag
     1021 atagcgtgcc gcgccgacca gcatggcggt gtgaccgtca tgaccgcagc cgtgcatcag
     1081 ccccggttgg gctgacttcc aggcaaagtc gttgtgctcg gtcattggca gcgcgtccat
     1141 gtcggcacgc aaaccgatca tctttgcact gctgttgcgc tggcccttga tgacggccac
     1201 cagcccggtc ttgccgatgc cggtgtgaat ttcatcgacc cgacacagct caagcgcctg
     1261 tttcaccctt gctgcggtgt aaacctcctc aaacccgagt tcaggatgag catgcaagtc
     1321 gcgccgaaac gctgtaagtt caggatgaaa tgccgcaatg tgcgcaaacg cacggccgtg
     1381 aaccatcaaa gtcatcaaaa ttccccaaag tccagaagca gtccgacttg cggttttagc
     1441 gcaaggagac gatttaacca tccacagcca caaaactctg ataccgggcg aaaaaagggc
     1501 cagcagcaaa tcgctccaaa gccctccaat agccagcatc agccggatca tctgatctaa
     1561 actgcacggt actacataca tcagcgttgc ctcatcatga tttttgtcac tggcggtgcc
     1621 ggctacattg gctcacacac ctgcgttgaa ctcctcaatg cggggcatca ggttaccgtt
     1681 ttcgacaatt tcagcaacag ccagccagaa gccttgaagc gggttgagcg catcaccggt
     1741 agcaggctgc aattgatcac aggcgatatc cgtgaccaga ctgcgctaga agcggcactg
     1801 agcgccagcc agccccaggc agtcatccat ttcgcgggac tcaaggcggt cggggaatcg
     1861 gtagcccaac cgttgaagta ttacgacaac aacgtggcag gcaccctcaa gctgctgcag
     1921 gcgatgcagg cgtgcgacat caggacgctg gtgtttagct cttcagccac ggtttatggc
     1981 gacccgcagc gcttgcccct gaccgaagac cacccgttgt cggccaccaa cccgtatggg
     2041 caaaccaaac tggtgatcga gaacatgctg cgcgacctgt ctgccagcga ttcttcgtgg
     2101 cgcattggca tcctgcgtta cttcaacccg gtcggtgccc atgccagtgg cctgattggc
     2161 gaggacccgc agggcttgcc caacaacctg ctgccttttg tggcacaggt ggcggttggc
     2221 cgacgcgcgt ttttgaacgt ctggggcaat gactacgcca cgccggacgg caccggggtg
     2281 cgtgactaca tccatgtggt cgatctggcg ctgggccact tggctgcgct ggcactgctt
     2341 cggggccacg caacgtgctt cgaggtcaat ctgggcaccg gcacaggtta cagcgtgctt
     2401 gacatggtgc gcgcctttga actggccagc ggcaagccgg tgccttacac catttcaccc
     2461 agacgcccag gcgatgtggc agcctgttat gccgacccgg cctttgcccg cgaactgctt
     2521 ggctggcacg cacagcgcga tctgcaaacc atgtgcctgg atgcctggcg ctggcagagc
     2581 ggcaacccaa acggctatag tgccgctagc tagcttgaga taagcgaaga tgaatcatga
     2641 caatgaaaaa aggatatcag catgactaag ggcatgattc tggcagccgg gcaaggtacc
     2701 cggattcggc cactgaccaa agatatgccc aagccgatga ttcccatttt gggtaagccg
     2761 gtgatggaat acctgattga gcacttggcc agctacggca tcaccgagat catggtcaac
     2821 gtggccttca accaccacaa gatcgagcag tattttggcg a
//
LOCUS       DAOSLL010000610         2855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_30472_length_2855_cov_3.871786, whole genome shotgun sequence.
ACCESSION   DAOSLL010000610 DAOSLL010000000
VERSION     DAOSLL010000610.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30472_length_2855_cov_3.871786"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="PLB25_19765"
     CDS             complement(<1..252)
                     /locus_tag="PLB25_19765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013596656.1"
                     /note="With MotB forms the ion channels that couple
                     flagellar rotation to proton/sodium motive force across
                     the membrane and forms the stator elements of the rotary
                     flagellar machine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HOE43842.1"
                     /translation="MFVIVGWVIVMVCVFGVYIFHGGNIMVVLKALPFELITIGGATA
                     GAFVANNQMKVIKSALKGLGRCFKGSKYTKARYMDLLALM"
     gene            complement(420..965)
                     /gene="flhC"
                     /locus_tag="PLB25_19770"
     CDS             complement(420..965)
                     /gene="flhC"
                     /locus_tag="PLB25_19770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HOE43843.1"
                     /translation="MATVSTKSVLNDSRQVERAVALIQLGARLQVLESETSLSYERLL
                     RLYKEVAGKSPSKGQLPFSTDWFLTWQPNIHASLFLNTYEYLNKVSALDDIDAIMKAF
                     KLYTEQIATCGVEPLLTITRAWRLVKFVDNHMLAMTKCSKCGGHFVSEPYENSRHFEC
                     GLCTPPARAGKGTGVGGILLH"
     gene            complement(977..1303)
                     /gene="flhD"
                     /locus_tag="PLB25_19775"
     CDS             complement(977..1303)
                     /gene="flhD"
                     /locus_tag="PLB25_19775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HOE43844.1"
                     /translation="MNDEQLSNDIRDANLTYLMLAQNVIRKDKAEALFRLGISEESAD
                     MIAMLSPAQILKIATSPMLLCRFRVDDDMVWSLLTNHTPAKVDNDATTKLHASILMAG
                     RFAEAL"
     gene            1857..2627
                     /gene="rfbF"
                     /locus_tag="PLB25_19780"
     CDS             1857..2627
                     /gene="rfbF"
                     /locus_tag="PLB25_19780"
                     /EC_number="2.7.7.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012430693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047343 - glucose-1-phosphate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate cytidylyltransferase"
                     /protein_id="HOE43845.1"
                     /translation="MKAVILAGGLGTRISEETHLKPKPMIDIGGKPILWHILKMYSAH
                     GVNEFVICCGYKGYVIKEYFANYFLHMSDVTFDMAANRMEVHERHAEPWRVTLVDTGE
                     DTLTGGRLKRVASYVKNDAAFCFTYGDGVADVDITASIAFHRQHGKLATVTAVQPPGR
                     YGALQMAGSCVNGFTEKPRGDGGLINGGFFVMSPRCLDRIEGDNTSWEAYPLTSLATD
                     GELMAFEHHGFWQPMDTLREKTQLEDLWQSGRAPWKVW"
     gene            2627..>2855
                     /locus_tag="PLB25_19785"
     CDS             2627..>2855
                     /locus_tag="PLB25_19785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006219019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glucose 4,6-dehydratase"
                     /protein_id="HOE43846.1"
                     /translation="MEPTSPRASTADFWSGKRVLLTGHTGFKGAWLALWLQRLGAEVT
                     GIALAPATRPSLFALLGDKPWMTSHLCDIRDA"
BASE COUNT          638 a          778 c          794 g          645 t
ORIGIN      
        1 catcaaagcc agcaggtcca tgtagcgcgc cttggtgtac ttgctgccct tgaagcaccg
       61 ccccaaaccc ttcagggctg acttaatcac cttcatctgg ttgttggcca caaaggcacc
      121 cgctgtggcg cctccgatgg tgatcagttc aaacggcaag gccttgagca ccaccatgat
      181 gttgccaccg tgaaagatgt acacgccaaa gacgcatacc atgacgatca cccaaccaac
      241 aatgacgaac ataaacgaat tgttttagag ggggctattg cggagaaatt gaactgccca
      301 attggagtgg gcttgtccct atcatatcgc ccggcagatg ccagtcctga gtttccaatc
      361 aaattggcat ctagccattg cgcagtatgc gctaatagct attcaaaata tagtgaatac
      421 tagtgcagca ggatgccgcc gacaccggtt cccttgcccg cgcgtgctgg tggggtgcaa
      481 agtccgcatt caaaatgccg cgagttctca tacggctcac tgacaaaatg cccgccgcat
      541 ttgctgcatt tggtcattgc cagcatgtgg ttgtcaacaa acttcaccag ccgccaggcg
      601 cgggtaatcg tcagcagcgg ctcgaccccg caggtggcaa tttgctcggt gtacaactta
      661 aaggctttca tgatggcatc gatgtcatcg agtgcgctga ccttgttcag atactcatag
      721 gtattcaaga acagcgacgc atggatattg ggctgccagg tcagaaacca gtcggtggaa
      781 aacggcagtt ggcctttgga gggcgatttc cccgccacct ccttatacag gcgcaacaaa
      841 cgctcatacg acaggctggt ttcgctctcc agcacctgca gccgtgcgcc gagctgaatc
      901 agcgctaccg cgcgctcaac ctgtctggaa tcattcaaaa cgcttttggt ggacacggtg
      961 gccatgatct gtctccttac aaggcttcgg caaagcgccc ggccatcaag atactggcgt
     1021 gcagcttggt agtggcatcg ttatcgacct tggctggcgt atggttggtc agtaggctcc
     1081 acaccatgtc gtcgtccacg cgaaagcggc acagcagcat cgggctggtg gcgatcttta
     1141 aaatctgcgc cggtgacagc atggcgatca tgtcggccga ctcttccgag ataccaagcc
     1201 gaaacagcgc ctcggccttg tccttgcgaa tcacgttttg cgccagcatc aggtaggtca
     1261 ggttggcatc gcggatgtcg ttggagagtt gctcgtcgtt catggtgggc tccagaaagg
     1321 ggttcaaaca tttggatggt gttttcggcc caattgtctt gttcaccacc ttgggataga
     1381 ttctggaagc cttcgacaaa aacttgaatc agtgtatggc tgaagcgtgt gtcggggttt
     1441 ggcgtgggtg atgtagggca aattctgaca ggcgaacatc gccgatcttt gggggtgcaa
     1501 ccaacaagtc accagaaaat tctggctttt caggcgctat cccttatgcg acaagcgctg
     1561 attgctatga aaaatagatc tcctgcacat caaatctaac cgcgctgtgg ctggccttgc
     1621 caaatgccac accaatttgg tgaatcggct gttccagcgc caggtaaggc atggcggagc
     1681 cctggcccag acggggtcat tagttggtac ccggaatctg tttttttgac ctcgttcccc
     1741 ttgtcccgct gaactgcgca cggaattaac ctcaaaaacc agacttgttg acgggctaag
     1801 ccggattcat tcggtcatat agttcaacca tgtcaatcaa ttgaactggc ggcgcgatga
     1861 aagcggtcat tctggcgggt ggtttgggca cccgaatatc cgaggaaact cacctcaaac
     1921 ccaagcccat gatcgacatc ggtggcaaac ccattctttg gcacattctc aaaatgtatt
     1981 cggcacatgg ggtcaacgaa ttcgtcattt gctgcggcta caagggctac gtcattaaag
     2041 agtactttgc caactatttt ttgcacatgt ccgatgtcac atttgatatg gcagccaacc
     2101 gaatggaagt gcatgaacgc cacgccgaac cttggcgcgt gacgctggtg gacactggtg
     2161 aagacacatt gaccggcggg cggcttaagc gcgtggccag ctacgtcaag aacgatgcgg
     2221 ccttctgctt tacctacggt gacggtgtgg ctgatgtgga cattaccgca tccatcgctt
     2281 ttcatcggca gcatggcaag ctcgccacag tgaccgctgt gcagccgccc gggcgttacg
     2341 gcgcgctgca aatggccggg tcatgcgtca atggcttcac cgaaaaacca cgcggcgatg
     2401 gtggcctgat caacggagga ttttttgtga tgtcgccgcg ctgcctggat cgcattgagg
     2461 gggacaacac cagttgggaa gcctacccgc taaccagttt ggcgacagat ggcgaactca
     2521 tggcatttga gcatcatggc ttctggcaac cgatggacac gctacgcgaa aagacccagt
     2581 tggaagacct gtggcaatcc gggcgagctc cgtggaaagt gtggtgatgg agccgacctc
     2641 gccccgcgca tcaacggctg atttttggtc tggcaagcga gtcttgctga ctggccacac
     2701 cggcttcaaa ggggcctggc tggcgctctg gctgcagcga ctgggggccg aagtgaccgg
     2761 cattgcactg gccccggcca cccggccgag cctgtttgcg ctgttgggcg acaaaccctg
     2821 gatgacgagc catctctgcg acatacgcga tgcga
//
LOCUS       DAOSLL010000611         2839 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_30711_length_2839_cov_6.278017, whole genome shotgun sequence.
ACCESSION   DAOSLL010000611 DAOSLL010000000
VERSION     DAOSLL010000611.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2839)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2839
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30711_length_2839_cov_6.278017"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="PLB25_19790"
     CDS             <1..505
                     /locus_tag="PLB25_19790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HOE43847.1"
                     /translation="QARRLLDLVLGKVGLDNKGTVKREWVGRDPAKIAAAIGLTLPSD
                     TRLLFVETAPDHPFAVTELMMPILPLIRVANVAEAIDLAVKLEGNCKHTAAMHSRNLD
                     NLDHMANVINTSIFVKNGPCLAGLGFGGEGWTSMTITTPTGEGVTSARTFVRQRRCVL
                     VDNFRIV"
     gene            1834..>2839
                     /locus_tag="PLB25_19795"
     CDS             1834..>2839
                     /locus_tag="PLB25_19795"
                     /inference="COORDINATES: protein motif:HMM:NF018032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HOE43848.1"
                     /translation="MSTRCIRSIGIDLGTTTSQVIFSELEIVNTAGPTSVPHYEFSRR
                     DILYVSPVIFTPFTADGQVEASSLDTFIRQQYALAGLTLQQVESGAVIITGETSKLRN
                     ARDTVLSLAAALGDFVVATAGPHLESVIAGRGSGAAAYSEKHAARVLNIDVGGGTSNY
                     AVFEGGRVTDTACLNVGGHLLQTDGSGQVSTVHAPAALILRELFGAGRTAAQLSAQDL
                     QQVTERMAALIIEVMLAQPSSLAQQLLMTDALRQAYRFDAVFVSGGVGECMLHPSGES
                     PYRFGDIGPLLAQALGRQLAEGGFAVREPTQTLRATVIGAGAHTLTLSGSTVWNRYQG
                     I"
BASE COUNT          526 a          860 c          883 g          570 t
ORIGIN      
        1 ccaggccagg cggctgctgg acctggtgct gggcaaggtc ggcctggaca ataaaggcac
       61 ggtcaagcgc gaatgggttg ggcgcgatcc ggccaaaatc gctgcggcca ttggtctgac
      121 gctgccctcc gatacccgtc tgctgtttgt cgagaccgca cccgaccacc catttgcggt
      181 gaccgagttg atgatgccga ttttgccgtt gatccgggtc gccaacgtgg ctgaagcgat
      241 tgacctggcc gtcaagttag agggcaattg caagcacacg gccgccatgc attcgcgcaa
      301 tctggacaac cttgatcaca tggccaatgt gatcaacacc agcatttttg tcaaaaacgg
      361 cccgtgcctg gcgggcctgg gctttggtgg cgaaggctgg accagcatga ccatcaccac
      421 cccgaccggc gagggcgtta cctcggcgcg caccttcgtg cgccagcgcc gctgtgtgct
      481 ggtggacaac ttccggatcg tctgaccgcc atgggatgca acacctgtcg cagcgcttgt
      541 ggcgactgtt ctgaaggctc cgtgagcttc aggcatcgag ccccccggcc gacgctgcca
      601 gcggcccggc ctgtcgggca aaccgggcgt gttcagcctg aaacgccgct gcgaaccgag
      661 caacccgcgg tgctgcagcc gactttaccg cgtgcggcaa agggggtctg acatggcagc
      721 cgtgtcgcca gcaattggca tgttctgcag tcgtatttcg accttgagcg gtaaggtttg
      781 cgacatggca gccaagtcgc ccgcaatggg caaattccgc aatcgtattc cggctttgag
      841 cggcaaggct tgcgacatgg cagccaagtc gcccgcaatg ggcaaattct gcccaagccg
      901 cttgggtggg tctgcccgca tcgggtttgc tcacattgct atttttataa gagctaacag
      961 cgcttgctgc ataagggctg gacggctaaa tgttcctcga accaaggcag gacgcggctc
     1021 gctgctggcc cgcagaattg gggtcagagc ccaattcggc gatgcccagt tggcaacttc
     1081 aaaagcgccg ggccgaattg gtatccgacc ccaaatctgc tacaccgcac ccgtttttgg
     1141 cgctggggca aaggaaggcc aatggatgtc tggtgacttc cgtgacgcaa actggccctt
     1201 gccaggcggc ttgaagcttg cccagcaggt cgctgccgcc attgacttat tcgtcaccca
     1261 gatcggtttt cagtaccact tccagtgtgc gcacgcggta gcagatttgg ggtcggatcc
     1321 caattcggtc cgagactttc aaatctgtca gcaatgcttg cccgaattgg gctcagaccc
     1381 caattctgcg gaccaccccg acagcgcttt ggtcagtgcg gcgcaaagtc gtgacagagt
     1441 tgcgatgaaa gcaaacgcgg tgcaagtgtc tgctgctatt tattttgtat ctggtggcgc
     1501 ttgctgcata agggctggac ggctaaatgt tcctcgaacc aaggcaggac gcggctcgct
     1561 gctggcccgc agaattgggg tcagagccca attcggcgat gcccagttgg taacttcaag
     1621 aaccctgggc cgaattggta tccgacccca aatctgctac gccgcacccg tttttggcgc
     1681 tggtgcaaag gcagtgccaa gcttgcctga caactctcgc ggagcaacca aatgcctgtc
     1741 agcccgcttg aagcttgccc gtcaagctga tgccgccgac aggttgtcca agcctgtcca
     1801 aggcagttac acaccaaccc agcaggccac gtcatgagca cacgttgcat ccgcagcatc
     1861 ggcatcgacc tgggcaccac cacgtcgcag gtgatttttt ccgagctgga aatcgtcaac
     1921 accgctggcc cgaccagcgt gccgcactac gagttttcac ggcgcgacat tctgtatgtc
     1981 agcccggtga tctttacccc gtttaccgcc gatggccagg ttgaagcgag ttcgctggat
     2041 acctttatcc gccagcagta cgcattggcg ggcctgacgc tgcagcaggt cgagtcgggc
     2101 gcggtgatca tcaccggcga gacctccaag ctgcgcaatg cgcgcgacac cgtgctgtcg
     2161 ctggcagccg ctctgggtga ttttgtcgtc gccacggccg ggccgcatct ggagtcggtg
     2221 atcgccgggc gcggttctgg cgcggcggcc tattccgaaa aacatgcggc gcgcgtgctc
     2281 aatatcgatg tcggtggcgg cacctcgaac tatgcggtgt ttgaaggtgg ccgggtgacc
     2341 gacaccgcct gcctgaatgt tggtggccat ttgctgcaaa ccgatggcag cggacaggtc
     2401 agcacggtac acgcaccagc cgcgctgatc ctgcgcgagc tgtttggcgc gggccgcacc
     2461 gcagcgcaac tgtcggccca ggatttgcaa caggtgactg agcgcatggc ggcgctgatc
     2521 attgaggtga tgctggcgca gccctctagc ctggcgcagc aactgctgat gaccgatgcg
     2581 ttgcgtcagg cctatcggtt cgacgcggtg tttgtctcgg gcggggttgg cgaatgcatg
     2641 ctgcacccgt cgggcgagtc accctaccgc tttggcgaca ttggcccgct gctggcgcag
     2701 gcgctggggc ggcaactggc cgagggtggt tttgcggtgc gcgaaccgac gcagaccctg
     2761 cgcgccacgg tgattggcgc gggcgcgcac accttgaccc tgtctggcag cacggtctgg
     2821 aacaggtacc agggcatcg
//
LOCUS       DAOSLL010000612         2837 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_30739_length_2837_cov_4.297987, whole genome shotgun sequence.
ACCESSION   DAOSLL010000612 DAOSLL010000000
VERSION     DAOSLL010000612.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30739_length_2837_cov_4.297987"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="PLB25_19800"
     CDS             complement(<1..631)
                     /locus_tag="PLB25_19800"
                     /inference="COORDINATES: protein motif:HMM:NF014763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOE43849.1"
                     /translation="MKPPEAPTPKIRTQWYLLWSVLLFLGLAISGILYQDYLRIEKEA
                     SATLSFRSVSGGHDLQRRLESLNTALNGLRRDTQFFKTAPHGLEIANRQMKALTESLE
                     GVRTLALFDAAGTMIASSRPEIVGQNFADRAYFTRPRAENNPSRLYVSAPFKTILGVY
                     SVILSKSLVDVNGEFAGIVTATLAPEDFSNILEGIRESPDAFAGIVHDSG"
     gene            complement(618..2786)
                     /locus_tag="PLB25_19805"
     CDS             complement(618..2786)
                     /locus_tag="PLB25_19805"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE43850.1"
                     /translation="MTIESSSTSGANSNGLEPVADELQVLRAWMQRSAIGLMVVNAQG
                     VIEFANPMAQKLCAGTPAQLLGQPCTALFAQALAPAETVAIQSSLANAQPWAVEAMIQ
                     RMDGTEFWGDFQLIPVADSLSGFTRWCVFLRDISHMVHDMDQAQHFDTLDQLTLLPNR
                     PQFLQYLQQLLGSAGAAGEYVLMAWIDVDHLRAINEAMGHAAADQVLLNIANLMREAS
                     RRQDLLARLGGDEFALVMSGASDTQVLQEAAERLIRSLQAKLVVPECAMKLSLTVGTA
                     IFPADARYVDDLMSCAEMALLEAKKEGDGTIRHYVTGFGKVGTSHTQVLAELRHAILT
                     GELRLFYQPQLSLHSGQIIGLEALVRWQHPTLGLLSPSHFITLAEECGLIIVLGEWVM
                     RTAVQQIRSWRNQGLTPVRVAVNLSAPHFRQKELPATIEKLLFDADVPASLLELELTE
                     SVMMHDAQAAIHIVNRLKTLGIRISLDDFGTGYSSLAYLSRFAIDVIKIDQSFVADIT
                     TNPVNASIATATIAMAHKLGKTVIAEGVETQGQLVYLRRHECDEIQGYLFSRPVDAAT
                     ISEMLRSGDGINLSLDQGETQMTLLLVDDEPNILNALKRLLRREGYHILSAESAAQGF
                     DLLATHRVHVIISDQRMPVMTGTEFLRRVKDLYPDTVRLILSGYSELESLTDAINKGA
                     IYRYISKPWDDERLKMDVTQAFRFYRERHPVSAVNDHEAT"
BASE COUNT          605 a          798 c          816 g          618 t
ORIGIN      
        1 ggccagagtc gtgcacaatg ccggcaaacg catcgggtga ctcgcgtatc ccctccaata
       61 tgttgctgaa gtcttccggg gccagcgtcg cagtcacgat gccggcaaac tcaccattga
      121 cgtccaccag gcttttggac aatatgacag agtaaacccc caaaatggtt ttaaagggtg
      181 cgctgacgta gagtctgctg gggttgtttt ctgcgcgtgg ccgtgtgaaa taggcgcggt
      241 cagcaaagtt ctgtccgacg atttccgggc ggcttgatgc aatcattgtt cctgctgcat
      301 cgaatagagc caaggtgcgt actccctcca gcgattcggt aagggctttc atttgccggt
      361 tcgctatttc cagcccatgc ggcgcagtct tgaaaaactg cgtatcccta cgaaggccat
      421 tcaacgccgt gttgagcgac tccagtcgcc gctgtagatc atgcccacca gagaccgaac
      481 gaaagctcag agtcgccgaa gcctcttttt cgatacgtag gtagtcttgg taaagaatgc
      541 cgctgatcgc caagcccagg aacagcagga cactccagag cagataccac tgcgtgcgga
      601 tttttggggt gggtgcttca ggtggcttca tggtcattca ccgcgctgac cggatggcgc
      661 tcacggtaga aacggaaagc ctgtgtcacg tccattttta acctttcgtc gtcccaaggc
      721 ttggagatgt agcggtagat ggcgcctttg ttgatggcat ccgtcaggga ttcaagttca
      781 gaatagcccg acaggatcag ccgcacggtg tcggggtaca agtctttgac acgtcgcaaa
      841 aactcggtgc cagtcatcac cggcatgcgc tggtcagaaa tgatcacatg cacgcggtgt
      901 gttgccagca ggtcaaaccc ctgggccgca ctttcagcac tcaggatgtg atagccctcg
      961 cggcgcagca gacgcttgag cgcgttcaaa atgttgggtt cgtcgtccac cagcagcagt
     1021 gtcatttgtg tttcgccctg gtccagactc agattgatgc catcaccgct gcgcagcatt
     1081 tcactgatgg tggctgcgtc cactggccgc gaaaaaaggt aaccctgtat ttcatcgcac
     1141 tcgtgacggc gcaggtaaac caattgcccc tgggtctcaa cgccttcggc aataaccgtt
     1201 ttgcccagtt tgtgggccat ggcaatggtg gcagtggcaa tggacgcgtt gaccgggttg
     1261 gtggtgatgt cggccacaaa cgactgatca attttgatga catcaatggc aaagcgcgaa
     1321 aggtaggcca gcgacgaata gcctgtgcca aagtcgtcca gcgaaatacg aatgcccagc
     1381 gtcttcagtc ggtttacgat atgaatggcc gcttgtgcgt cgtgcatcat gacgctttcg
     1441 gtcagttcca gctccaacag ggatgccggc acatcagcgt caaacagcag tttctcaatt
     1501 gtcgctggca gttctttttg gcggaaatgc ggggctgaca agttgacggc cacccgcacc
     1561 ggggtcaagc cctggttgcg ccaactccga atttgttgca ccgctgtgcg cattacccat
     1621 tcaccaagca caatgatcag cccacactct tcggccaatg tgatgaagtg agatggactg
     1681 agcaatccga gtgtagggtg ctgccagcgt accagggctt ccaggccaat aatctgaccc
     1741 gagtgcagac tgagctgcgg ctggtaaaac agccgcagct cacccgtcaa gatggcatgg
     1801 cgcagttcgg ccaagacttg ggtgtgggag gtgcctactt tcccgaagcc ggtgacgtaa
     1861 tggcgtatgg taccgtcgcc ctcctttttg gcttccagca gggccatttc agcgcacgac
     1921 atcaggtcgt cgacatacct ggcatcggct ggaaaaatgg ctgttccgac ggtcagactc
     1981 agtttcatcg cgcactctgg cacaacgagc tttgcttgca ggctgcgaat cagacgctcg
     2041 gccgcctctt gcaatacctg ggtgtcgctg gcgcccgaca ttaccagcgc gaactcgtca
     2101 ccgcccagcc gtgccagcag gtcctggcgg cgtgatgcct ctcgcatgag gttggcaatg
     2161 ttgagcagta cctgatcggc agcagcatgc cccatggctt cattgatggc gcgcaggtgg
     2221 tccacgtcga tccaagccat caacacatat tcgccagccg caccggcgct gcccaagagc
     2281 tgctgcagat attgcaggaa ttgcggccgg ttgggcagca gcgtcagctg atccaatgtg
     2341 tcaaagtgtt gcgcttggtc catgtcatgc accatgtggc taatgtcgcg caggaagaca
     2401 caccagcgtg taaagcccga caacgagtca gctaccggaa tcaactgaaa gtcgccccag
     2461 aactcggtgc cgtccatacg ttgaatcatg gcctccacag cccacggctg cgcgttggcc
     2521 aagctgcttt gtatggccac ggtttcggca ggcgcaagcg cctgggcaaa aagcgccgta
     2581 caaggctggc ccagcagctg ggctggcgtg ccagcgcaaa gtttttgcgc catggggttg
     2641 gcaaactcga tgacgccttg cgcgttgacc accatcagcc cgatggccga gcgctgcatc
     2701 caggcgcgca gcacctgcaa ttcgtccgca accggttcaa gaccgtttga gttggcacct
     2761 gaagtggaag aagattcgat ggtcataggt cactttgctc atggcaatgt gagttctgtc
     2821 gggtggcgcg gcaagat
//
LOCUS       DAOSLL010000613         2828 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_30856_length_2828_cov_5.129463, whole genome shotgun sequence.
ACCESSION   DAOSLL010000613 DAOSLL010000000
VERSION     DAOSLL010000613.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30856_length_2828_cov_5.129463"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(114..1379)
                     /locus_tag="PLB25_19810"
     CDS             complement(114..1379)
                     /locus_tag="PLB25_19810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003064490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE43851.1"
                     /translation="MTDQTEPVTSPANAAVASLAIARQPILDVKQAVFGYELFDRSLG
                     STPYSAASDAALLFNLLSTVDTEILSTRHPLFINCTHQTLAGGHLDLIEPDRVVLEIP
                     PMPADQCEAEDIDTFGQTLADLHRRGFRLAFDESVMTAPYGTWLALTSFIKLDITKVS
                     QQQIATLARQLIGNTKIQLVAEKVETAEQFARVQLAGVKLFQGYWFARPVLIKGQTIR
                     PAQAAIIQLINLVRRQASTTEIEEVFKRAPTLSFNLLRFINSAGFGLRCEVTSFRHAV
                     MLLGLKKLFRWAALLMTTSGSTGGSPAVGQVAVVRGRLMELLAAELLPAEEHDNAFVV
                     GVFSLLDTMLGLPLEEALASISLPKTVTDALLHRTGVLAPFLQLTLACENADDKGFAQ
                     NAIALQLTGDQVNWAHLQALAWAENITGT"
     gene            complement(1402..2199)
                     /locus_tag="PLB25_19815"
     CDS             complement(1402..2199)
                     /locus_tag="PLB25_19815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011515848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOE43852.1"
                     /translation="MSLRTTSLAVLQICDAFKKCAATSTLPCHVGAVDASLVFEEPAA
                     LPGRPDRPTLVPMLKVQQRSMQTIEGRAALIHALAHIELNAIDLALDIVWRFPGMPDQ
                     FYLEWASVAQEEARHFTLLADHLSALGFQYGDFLAHNGLWAMAEKTSHDLLARLAIVP
                     RTLEARGLDAAPPVRDKLLSVGDKRGAEIVSIILQDEIGHVATGNRWYRWLCDQRGLD
                     PITTDKIIAVQYGAPRPRGPFNLPARRAAGFEECELSALCGTPAPDL"
     gene            2402..>2828
                     /locus_tag="PLB25_19820"
     CDS             2402..>2828
                     /locus_tag="PLB25_19820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003057724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HOE43853.1"
                     /translation="MAIYEIDGLVPQIADSAWVADNAQVMGDVVLGAEVGIWFGVVIR
                     GDTDRIRIGARTNIQDLSVLHADLGKPLTIGSGVTVGHKAMLHGCTIGDDSLIGIGAV
                     VLNGAVIGKGCLVGAGALVTEGKEFPDGSMIIGSPAKVVR"
BASE COUNT          592 a          775 c          871 g          590 t
ORIGIN      
        1 aaacaagcgt cttgcaacga cgctgtgcgc ccaaatccgc cggtgcaaat agggcaagct
       61 atttctgatc gcatcctcag gccatcgcgt ccacctgggg gccgtgtgct tgctcaggtg
      121 ccggtgatgt tctcggccca ggccagggcc tgcagatggg cccagttgac ctggtcgccg
      181 gtcagttgca gtgcaatggc gttctgcgca aaccccttgt cgtcggcgtt ttcacaggcc
      241 agcgtcagtt gcaagaacgg agccaatacc ccggtgcggt gcaacagggc atcggtgacg
      301 gtctttggca aggaaatgct ggcgagtgct tcttcaagtg gtaaccccag catggtgtcg
      361 agcaaagaaa acacgccgac gacaaaagcg ttgtcgtgtt cctcggctgg cagcagctca
      421 gcggccagca gttccatcag acgtccgcgc accaccgcaa cctgacccac tgccggtgag
      481 ccaccggtcg agcccgaggt ggtcatcagc aatgcggccc agcggaacag ttttttcagg
      541 cccagcagca tgacggcgtg gcgaaatgat gtgacttcgc agcgcaggcc aaaaccggcc
      601 gaattgatga agcgcaacag gttgaatgac aaggtcgggg cacgcttgaa gacttcttca
      661 atttctgtcg tgctggcttg cctgcgcacc aggttgatga gttgaatgat ggccgcctgg
      721 gccgggcgaa tggtctggcc cttgatcagc accggcctgg caaaccagta gccctgaaac
      781 agcttgacac cagccagttg gacccgcgca aactgctcgg cggtttcgac cttttccgcc
      841 accagttgaa tcttggtgtt gccgatcaac tgccgggcaa gggtggcgat ttgctgttgc
      901 gacaccttgg tgatgtcgag cttgatgaat gaggtcagcg ctagccaagt gccatagggg
      961 gccgtcatga cgctttcgtc aaaggcaagc cgaaagccgc gcctatgcaa atcggccagg
     1021 gtttgcccga aggtgtcgat gtcttcggcc tcgcactgat ccgcaggcat gggcggaatc
     1081 tctagcacca cccggtctgg ctcaatcaga tccagatgcc cgcccgccag cgtttgatgg
     1141 gtgcagttga taaacagcgg gtggcgggtg ctcaagattt ctgtatcgac agttgacaac
     1201 aggttgaaca gcagggccgc atcgctggcc gcgctgtagg gggtgctacc gagcgagcgg
     1261 tcaaacaatt cgtagccaaa caccgcctgt ttgacatcaa gaatcggttg gcgggcgatc
     1321 gccagcgatg ccacagcggc attggcaggg ctggtaacag gttcagtttg atcggtcata
     1381 gtttgaaaat gatccagtgg ttcagaggtc aggcgctggt gtaccacaca atgcgctcag
     1441 ttcgcattct tcaaagcccg ccgcccgccg ggctggcaga ttgaatgggc cacggggcct
     1501 gggtgcgcca tattggacag cgatgatctt gtcggtcgtg atcgggtcca gaccgcgctg
     1561 gtcgcacagc caccggtacc agcgatttcc agttgcaaca tggccaatct catcttgcaa
     1621 aatgatcgag acaatttcgg caccgcgttt gtcgccgact gacaacagct tgtcgcgcac
     1681 cgggggtgcc gcatcaaggc cacgtgcctc cagcgtcctg ggaacaattg ccaggcgggc
     1741 caacaggtca tggctggttt tttctgccat ggcccaaagt ccgttatgcg ccagaaagtc
     1801 tccgtattga aatcccaagg cggacaaatg atcggccagc aaggtgaagt gtctggcttc
     1861 ttcctgcgcc acgctggccc attccagata aaactgatcg ggcatgccgg gaaaccgcca
     1921 gacaatgtcc aaagccaggt caatggcgtt gagttcgata tgcgccagtg catgaatcag
     1981 ggccgcgcga ccctcaatcg tctgcattga tcgttgctga actttcagca tcggcaccag
     2041 cgttggtcga tcaggtcggc ccggcagtgc agccggttct tcaaatacca ggctggcatc
     2101 gaccgccccg acgtggcagg gcagtgtgct ggttgcggca cactttttga acgcatcgca
     2161 aatttgcaac acggcaagtg atgtggttct gagagacatt gatggcgttg cggcgggtta
     2221 ctgcttgttg ctgattgtgg caagaacggc accagccaag atgttcaaac gcttggccct
     2281 gtttgacaat ggcagcgatt ggcgctgctg tcattgtcaa acacggtcgg ctgttggcaa
     2341 gcgagtattt tcatgccaat gcaaacctac aattccgcac cgatgaacac aaaggacaat
     2401 catggcaatc tatgaaatcg acggcctggt gccgcaaata gcggattctg cctgggtggc
     2461 agacaacgca caagtcatgg gcgatgtggt actgggtgca gaagttggca tctggtttgg
     2521 cgtggtgatt cgtggtgaca ccgaccgcat ccgcattggg gcgcgcacca atatccagga
     2581 tttgagcgtg ctgcatgccg atctgggcaa gccgctgacg attggctcgg gtgtgacagt
     2641 tgggcacaaa gccatgctgc atggctgcac gattggcgac gacagcctga ttggcattgg
     2701 cgcggtggtg ctcaatggcg cggtgattgg caagggctgc ctggttgggg ccggtgcact
     2761 ggtcactgaa ggcaaggagt ttcccgatgg cagcatgatc attggcagcc cggccaaggt
     2821 ggtgcgcc
//
LOCUS       DAOSLL010000614         2825 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_30904_length_2825_cov_3.585199, whole genome shotgun sequence.
ACCESSION   DAOSLL010000614 DAOSLL010000000
VERSION     DAOSLL010000614.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30904_length_2825_cov_3.585199"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            433..633
                     /locus_tag="PLB25_19825"
     CDS             433..633
                     /locus_tag="PLB25_19825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43854.1"
                     /translation="MSIIFFLRSLFRTPEFRVGGRVNAIRDGHIERSDGYVVAQTDLG
                     VLVEWPCGGTSVVSASDLSVIG"
     gene            complement(2021..>2825)
                     /locus_tag="PLB25_19830"
     CDS             complement(2021..>2825)
                     /locus_tag="PLB25_19830"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOE43855.1"
                     /translation="SDIRNPFFTEISRAVEDVAYQNRMRVFLCNADENPDKEAFYLQM
                     MRDENVSGVILSPTPTLLKHLKTGDYPFPLVLVDRCERDTDADAVVLDNFDASHRLTE
                     HLIAQGCRSIVFLYGSVGSTGPQRHQGYLAAMLAAGLEPQAEPIAPHSERAREVAALL
                     LQRRPLPDALVASSGLVLLGIAEAIKAAGVRVPADTAIAGFDDLPWTSLMTPDITVMR
                     QPTNEIGQAAIRLLLERVALPERAVRRVVLRGELLVRGSTQIQTDGIDQ"
BASE COUNT          596 a          793 c          807 g          629 t
ORIGIN      
        1 ctttgtggat ggcctacgcg cacaacgacg cgccgtggtg cacaccaatc tgaatttccg
       61 aaatcaggca tgcgctgcaa ccccgctcag ggtgctgtaa gcctagtgcg ccgctgattt
      121 tgccaatcgc caagcaagga gtttttcggc gttggtttgc acacataatt cggctttagc
      181 cttagcgggt taagcggagt tcgctattaa aactgtagtt tttcgttacc tcaaagtcgc
      241 ctgatcaggc ctccttattg gtcaatgtcg gttcagttgg tttgtttttc gtgtcagaaa
      301 caacatattc cgtcgagttg agcaggcatt ggggtcactc gaatcagatc tcatgcaata
      361 ctagggttaa cccttgttta ttagggtaaa ccctaatttt ggggtctttt ttatgtcggg
      421 agactttcaa tcatgagcat catttttttc ttgagatcac tttttcgcac ccctgagttc
      481 cgggtaggcg ggcgcgtcaa tgccattcgc gatggccaca tcgagcgctc ggacggttat
      541 gtcgtcgcac aaaccgacct cggtgtgttg gtcgaatggc catgtggcgg caccagcgtg
      601 gtgtcggcat cagacttgtc tgtcatcggt tgaacgacgt gggttttccg gtagccgtgg
      661 ccgctgattc cgaccgatca tggtcgctga catgccgcgc tgtggtcggc tcgcagccca
      721 tgcacccgaa tttcacagct tcacaggcgc atgacgaagg cggtgccggg caacttacag
      781 gtgcggccta tgccccagag tcgactggat gtggaaggca gcgcgcgggt ttggcttgtc
      841 cagccgggcg caatgtggcg tatcagcaat tggcaagcac cgaagcggtc aggccagagg
      901 cccagccgga tgccacctgg gtccgcgcgc tcaatatcag cataaccgcg ctggcaccca
      961 ggtttgacca gccttcacaa ccaggaatat ggcaaaacag ccttcccgcc cttcagagac
     1021 gggcgttggt agctcacaaa ttgattgcaa cagggcaact tgactgggtc gccgagcatc
     1081 ctttgctgtg cactgcaggg ccatctgctt cattgcgcgc agacccttgt cagcgagtgc
     1141 aagagcgagc gactcaagtg tcattccatt tctaaatcat ccatgtcgtt gttatctcgc
     1201 cttgccttgc gtttgcaatg tctgcggctc aatgcctgca cacgaatgat ctagaaatgg
     1261 aatcaggcgg cgatttgtcg gctttgctac ctgctcggct ggacatcccc tcagcctcgc
     1321 catttactat gcttttggct tgagaagcgg cagcctgttg aaattggctg aggttcaata
     1381 ccgtcgtcca aatgtcagca ccgcttcgca tggtcatatt ggcttcaacc tggcttgcgc
     1441 agtaggtcga acgccagtgg gctgctggcg gcggggctgg gtaggagcga ctacgacacg
     1501 agcgactgcg cgcatgtcgt ccttgcggac gagcaaggcg taagcgcacc caggcgagtt
     1561 gccagcggtt caatcaggtg cgtcgcccgg taactcgttg ggtgattgcc tttgaagcca
     1621 caaaagtaaa tgcgcacagg gaacagatac attgcctaca atcaaaataa tagctagaaa
     1681 cgcttaatcc ataaggggta aaggccgatt tggcagataa aaactgggcg gcttgcaccg
     1741 ccagcggcat cagctccaac cccatgtttc tttgacaacg ccagacccac gcgcgggttg
     1801 gggtgctcac aaggactgat tccattccca gatcatgcgt gtccagtcac cttggcgcag
     1861 acattgcaaa cgcaaggcga ggcgagaaaa caaggacatg gctgatttgg aaatggacgg
     1921 acagatgaat accagcgctc aacggagtaa tcaaggttga agcaaattgt cgttagtttg
     1981 tcgggactgc caattgcaaa agctcggtgg caaaagcgct tcactggtcg atgccatcgg
     2041 tctgaatttg cgttgatccc cgcaccagca attcgccacg cagcaccact cgccgcaccg
     2101 cccgctcggg caaggccact cgctccagca gcagccggat ggccgcttgg ccgatttcgt
     2161 tggtcggctg gcgcatcacg gtgatgtcag gtgtcatcag gctggtccag ggcagatcgt
     2221 caaaaccggc gatggccgta tctgctggca ccctgacccc ggcggccttg attgcctctg
     2281 caatgcccag cagcaccagt ccgctgctgg ccaccagcgc gtccggtagc gggcggcgct
     2341 gcagcagcag cgcggccacc tcgcgtgcgc gctcgctgtg tggtgcaatt ggctcggcct
     2401 gcggctccag gccagccgcc aacatggccg caagataacc ctggtgccgc tgcggcccgg
     2461 tcgagccgac cgaaccataa agaaaaacga tgctgcggca gccctgcgca atcaggtgct
     2521 cggtcaggcg gtggctggca tcaaaattgt ccaataccac cgcatccgca tcggtatcgc
     2581 gctcgcaacg atccaccagc accagtggaa aaggatagtc cccggttttc agatgtttga
     2641 gcaaagtcgg ggtgggcgag aggatgacac cgctgacgtt ctcgtcacgc atcatctgca
     2701 ggtaaaaagc ctccttgtcc gggttttcgt cggcgttgca caaaaacacc cgcatgcggt
     2761 tctggtaggc cacatcttcc acggcccggc tgatttcggt gaaaaacggg ttgcgaatgt
     2821 cagat
//
LOCUS       DAOSLL010000615         2822 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_30942_length_2822_cov_4.479219, whole genome shotgun sequence.
ACCESSION   DAOSLL010000615 DAOSLL010000000
VERSION     DAOSLL010000615.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30942_length_2822_cov_4.479219"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(305..381)
                     /locus_tag="PLB25_19835"
     tRNA            complement(305..381)
                     /locus_tag="PLB25_19835"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(345..347),aa:Arg,seq:acg)
     gene            complement(522..1376)
                     /gene="thiD"
                     /locus_tag="PLB25_19840"
     CDS             complement(522..1376)
                     /gene="thiD"
                     /locus_tag="PLB25_19840"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HOE43856.1"
                     /translation="MHTERIHHYPRVLSIAGSDSGGGAGIQADLKTFAALGCFGMTAI
                     TALTAQNTCGVRAIHGVPPDMLRDQIDAVLEDIGVDAVKIGMLHSAEVVRTVAQAINR
                     HQLPQVVFDPVMVATSGAVLIDQAAIEVLVSEMFARVVLITPNLEEAGLLVGRPLTSV
                     ADMALAAQELLARGARAVLLKGGHLPGQVVVDLLLADSGEPLWMEAPRINTANSHGTG
                     CTLSSAIAAYLALGLALPDAVQQARSFVRAALVAGANVRTGQGSGPLNHGFAPLATRV
                     LTGSVASR"
     gene            complement(1722..2369)
                     /locus_tag="PLB25_19845"
     CDS             complement(1722..2369)
                     /locus_tag="PLB25_19845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HOE43857.1"
                     /translation="MAAPSLAPRALYEKVAEMLRQRIFSHELEPGGWIDELKIAEAYG
                     ISRTPLREALKVLAAEGLVTMKIRRGAYVTEVSERDLADVYHLLGLLESDAAGAAAQV
                     ASDAQLTELQTLHERLEQTAQPASQDRELFFEINEQFHMRLLDIANNRWRKQMVADLR
                     KVMKLARHNSLLKGGRIYESLKEHRTLMAALLARDGAQCQMRMQEHFKNGLDAAN"
     gene            2501..>2822
                     /locus_tag="PLB25_19850"
     CDS             2501..>2822
                     /locus_tag="PLB25_19850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012347718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HOE43858.1"
                     /translation="MTQNTPEFQPSDLSSWAKAAAKSAPGGDVAALNWQTPDGISVKP
                     LYTAADTKDLPYTDTLPGFEPFLRGPQATMYAVRPWTIRQYAGFSTAEESNAFYRKAL
                     AAGGQ"
BASE COUNT          628 a          827 c          773 g          594 t
ORIGIN      
        1 cgccttcgcg gtagctgatc aagcccttgc gcagcgttgt tcatatgcaa aaccagcctc
       61 tagcccccgt actcaatgca cagagagata tcacattaat agcaagctgc tgagcgtcga
      121 acgtaaggaa gaccggcatc atcgatggcg tgcagccatt tggggttagt tcagccctca
      181 acccagcagg tctggtactc aaagaaagtt tcgagaaacg gcaaccgaac ggcaaccaga
      241 acgatgtcaa aagccacaaa gaagaaagga cttagagctc ttgactctaa gtccttgatt
      301 catttggtgc ggctggcagg aattgaaccc acgacccctt ggttcgtagc caagtactct
      361 atccaactga gctacagccg cttgttgttg accagattct accatccaac ctgacgttca
      421 ttttcaaaaa aagcatcact tgccgattga accatctggt caatttggtt gcgtgagccg
      481 agcaatcaac gccgggcaag cacgcttggg ccgacaggcg attagcgcga agccacagac
      541 cccgtcaaga cccgtgtcgc caatggcgca aacccatgat taagcgggcc actgccctga
      601 ccggtcctga cgtttgcgcc agcaacaagg gcagcgcgca caaaagaccg tgcctgctgc
      661 accgcgtccg gcagcgccaa acccaacgcc aggtaggccg caatggcact tgacaaggtg
      721 caaccggtgc catggctgtt ggctgtgttg atgcgcggcg cttccatcca cagtggctcg
      781 cccgaatcgg ccagcagcaa atccaccacc acctggcccg gcaaatgccc gcctttgagc
      841 aacaccgctc tggccccacg cgccagcagt tcttgagcag ccagtgccat gtctgccacg
      901 ctggtcaggg gccgaccaac cagcagtccg gcctcttcaa gatttggggt gatcagcacg
      961 acgcgcgcaa acatctcgga caccagcacc tcaatggcgg cctgatcaat cagcactgcg
     1021 ccgctggtag ccaccatcac cggatcaaaa accacttggg gcagttgatg gcggttgatt
     1081 gcctgtgcca cggtgcgcac cacttcggct gaatgcagca tgccaatctt gacagcatcg
     1141 accccgatgt cttccaggac tgcgtcaatt tggtcgcgca gcatgtcggg tggtacaccg
     1201 tggatggcac gcacgccgca agtgttctgc gcagtcagcg cggtaatcgc cgtcatgcca
     1261 aagcagccca gcgcagcaaa ggttttcaga tctgcctgga taccggcacc gccaccgctg
     1321 tcggaacccg caatcgacaa aactcggggg taatggtgaa ttcgttcggt gtgcatgagt
     1381 gggtgagctt ggtctggcga ccggacaatg ctgggtttat tggcgtcaat cgggaattcc
     1441 agtggatgtg gcgataaatc gtgcgccgtg gccagtcgaa ttgattggtt tataccgcaa
     1501 tgtcgtgccc ggcgagtcca cgatccacgc gtgtgttcat gcggcacctt tcacttgcca
     1561 ctgcactggg gtgaccaatg ccgtggaact tggcggagtt gctgctggcg ttgggcaacg
     1621 cgtggctgat gcctgagcag ctcaagacac ctgacggaaa tggcgagcac ccaagcgggt
     1681 ccctggcggc gcgcctaaga agcaggaaat gctcctgcaa actaattggc ggcatccagg
     1741 ccgtttttga aatgctcttg catgcgcatc tggcactgtg cgccatcgcg ggccagcaac
     1801 gcggccatca aggtgcgatg ttccttgagg gactcataaa tgcggccgcc tttgagcaaa
     1861 gagttgtgcc gggcgagttt catgaccttg cgcaagtcgg ccaccatctg tttgcgccag
     1921 cggttgttgg caatgtcaag cagccgcatg tgaaactgct cgttgatctc gaaaaacaac
     1981 tctctgtcct ggcttgctgg ttgtgctgtt tgctccaggc gttcgtgcag agtttgcaat
     2041 tcggtcagtt gtgcatcact ggcgacttgc gctgccgcac ccgcagcgtc ggattcgagc
     2101 agacccagca aatggtaaac atcggccaga tcacgttctg acacctcggt cacgtaagca
     2161 ccgcgcctga tcttcatggt caccagccct tcggctgcca gcactttcag ggcttcgcgc
     2221 aggggtgttc ggctgatgcc ataggcttcg gcaatcttga gttcgtcaat ccagccacct
     2281 ggctccagct cgtgactgaa aatacgctgg cgcagcattt cggcgacttt ttcataaagt
     2341 gccctgggag ctaaagacgg tgcagccatg cctcgaattg taagttggca ggaatttttt
     2401 taatcaataa ttataaataa cgtaaacttc tgcgttcgat cgcgacgctt catgaacagc
     2461 gagagaatgt cggcatttca ccaaccaaat acgtacaacc atgacacaaa acacacctga
     2521 gttccagccg tctgatctct cttcgtgggc taaagcggcc gccaagtcag cacccggtgg
     2581 cgatgtggcc gcacttaatt ggcagacacc tgatggcatt tccgtcaagc cgctttacac
     2641 cgcagccgat acgaaagatc tgccctacac cgacaccttg ccggggtttg agcccttttt
     2701 gcgcggccca caagccacca tgtatgcggt gcgtccgtgg acgattcgcc agtacgccgg
     2761 tttttccacg gctgaagaat ccaacgcgtt ttaccgcaag gcgcttgcgg caggtgggca
     2821 gg
//
LOCUS       DAOSLL010000616         2821 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_30964_length_2821_cov_3.140998, whole genome shotgun sequence.
ACCESSION   DAOSLL010000616 DAOSLL010000000
VERSION     DAOSLL010000616.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30964_length_2821_cov_3.140998"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="PLB25_19855"
     CDS             <1..1309
                     /locus_tag="PLB25_19855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIGR03013 family PEP-CTERM/XrtA system
                     glycosyltransferase"
                     /protein_id="HOE43859.1"
                     /translation="SLLMLVVLSMVLSNAGDPTSAYPSFLATFFFLATGLLLLSAAFG
                     FYRRGSNRSIAQAASRSLLVWLLMLPLIYSVAHRMQQPRTFGLTLALMVLLLLVLRVY
                     AMHAKKSAMLRQRVLVFGIGERAKMVGAALKHADPYVDLVGYYAGPNESDARVTAWGL
                     LSMTNSLTDIVMEEQVDVIVVALSERRGGAMPMRELLDCKLQGVRVMDIATHFERTLG
                     QIRLESVSAGWLIFGDGFGSGWVRSIVKRAFDIVCSAVLLLLTLPVMLVTAILIVLES
                     GFPVLYFQERVGLNGRLFNVVKFRSMRSDAEKDGQPRWASAKDDRVTKIGRVIRKLRI
                     DELPQLFSVLGGTMSLVGPRPERPFFVDKLTEQIPYYAVRHSVKPGVTGWAQIRYQYG
                     ASVEDSAEKLQYDLYYVKNHSLFLDILILFETVGVVLMRKGAQ"
     gene            1361..>2821
                     /gene="prsK"
                     /locus_tag="PLB25_19860"
     CDS             1361..>2821
                     /gene="prsK"
                     /locus_tag="PLB25_19860"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HOE43860.1"
                     /translation="MEADGLGLAGWGFALVGLTYSAFALRLVQLGGLRAPRELSKVAV
                     LAAVVWTALWGWFGLISVESPKPVFLLLSTLSDQFRYACWFAFLLSLLRANKLQSDAK
                     EKPWMAVFAAALTAFSVSVLLKQMLGVGNLSGSTRLMWLCSMTLPLLAMVLLEQVFRN
                     ATEDSRWNIKPLSIGLAGTFLFDLYLFSQAALFNRPDGDALNIRSVLHALMVPLLLLS
                     SIRRSDWTSKIHISPKAAFHSATLFLTGIYLLFISGVGYYVRYFGDEWGRALQLGLVF
                     LALVILMILALSGSLRAKLRVFLGKHFFRYRYDYREEWLRFTRALSVRNSPQEMGLQV
                     IRGLAEMLESPAGGLWLRNKGDAAFSQMARWNQAQASAKEDLDSPLCQFMKTGGWVIN
                     LEEFRSFSRRYGELKLPSWLQELPQAWLLVPLLVGDDLIGFVVLASARTRVDVNWEVN
                     DLLKTAGQQAASYLAQMQATEALLEVRKFDAFNRMSA"
BASE COUNT          526 a          691 c          862 g          742 t
ORIGIN      
        1 gagtttgctg atgctcgtag tgctgtctat ggtgctgtca aatgctggtg acccaacttc
       61 tgcttacccg tcatttctgg caacattttt cttcttggcg actggactgc tgctgctaag
      121 cgcagcgttc ggcttttacc gacgtggcag caaccggtca atcgcccagg cagcatcgcg
      181 cagcctgctc gtgtggttac tgatgttgcc tttgatctat agcgttgcgc accgcatgca
      241 acagcccaga acctttgggt tgacccttgc cctgatggtg ctgctgttgc tggtgctgcg
      301 cgtctatgca atgcatgcca aaaaatcggc catgctgcgt cagcgggtgc tggtatttgg
      361 tatcggtgag cgcgccaaga tggtcggtgc cgccttgaag catgctgatc cctatgtcga
      421 tttggtcggg tattacgctg ggccaaatga gtctgatgcc agagtgacag cctgggggct
      481 gctgtccatg accaactctc tgacagatat tgtgatggaa gagcaagttg acgtgattgt
      541 ggtcgctttg agcgagcgac gcggtggtgc catgcccatg cgtgaattgc tagattgcaa
      601 gctgcagggt gtgcgagtga tggacatcgc cacgcacttt gagcgtacct tggggcaaat
      661 tagattggag tcggtatcag ccggatggtt gattttcggt gatgggtttg gatcgggctg
      721 ggtacgcagt atcgtcaagc gagcttttga cattgtttgc tctgctgtcc tgttgctgct
      781 aaccttgccc gtcatgttgg tcacggccat tttgatcgtg cttgagagtg ggtttccggt
      841 gctgtatttt caggaacgag tggggctcaa tggcaggcta tttaatgtag tgaagtttcg
      901 cagcatgcgc tccgatgccg aaaaagatgg ccaaccgcga tgggccagcg ccaaggatga
      961 ccgagtaacc aaaatcggca gggtgattcg caagctgcgc attgatgagt tgccacagtt
     1021 gttcagcgtt ctcggtggga ccatgagcct ggtcggcccg cgcccggagc ggccgttttt
     1081 tgtggacaaa cttaccgagc agattcccta ctacgcggtg cgccacagcg tcaaaccagg
     1141 tgtcacaggg tgggcacaaa ttcgatacca gtatggcgca tcggttgagg actcggctga
     1201 aaagttgcag tacgacctgt actacgtcaa aaaccactcg ttgttcctgg atattctcat
     1261 cttgtttgaa acggtgggtg ttgtgctaat gcgcaaaggc gctcagtagc ggctgtgtca
     1321 gggcaaacca caatgcaaac tgaggcggtg gttttcacgc atggaagctg acggattggg
     1381 tttggccggg tggggtttcg cgctggttgg actgacctat tcagcatttg cgctgcggct
     1441 ggtgcagctt ggtggtctac gtgccccgcg cgaattgtca aaagtagcgg tgctggcagc
     1501 ggtggtctgg actgcattgt ggggctggtt tggcttgata tcagttgaga gtcccaaacc
     1561 tgtctttttg cttttgagca ccttgtccga ccagttcagg tacgcttgct ggtttgcgtt
     1621 tttgctgtca ctgctgcgtg cgaacaagct gcaatcggat gccaaggaga agccctggat
     1681 ggcggttttc gcggcggcac tgaccgcgtt tagcgtgtca gtgttgttga agcagatgct
     1741 gggtgtcggc aatctgagtg gctccacccg cctgatgtgg ctgtgttcga tgacattgcc
     1801 actgctcgcg atggttttgc ttgagcaggt ctttcggaat gccacggaag actcgcgctg
     1861 gaacatcaag ccgctgagta ttggcctggc cggcactttt ctgtttgacc tgtatctgtt
     1921 ttcacaagct gcgctgttca accgtcctga cggcgacgca ctcaacatcc gcagtgtcct
     1981 ccatgcgctg atggttcccc tgctcctttt atcgagcata cgacgcagtg actggacttc
     2041 aaaaattcac atctcaccca aggccgcatt tcactcggcg acgctgttct tgaccggcat
     2101 ttacttgctg tttatttcag gtgttggtta ttacgtgcgc tactttggcg acgaatgggg
     2161 ccgagcgctg caattgggcc tggtgtttct tgcactggtc atcttgatga ttctggcgtt
     2221 gtcagggtct ttgcgggcaa agttgagagt gttcttgggg aaacattttt tccgctatcg
     2281 ttacgattac cgcgaagagt ggctgcgatt cacacgtgcc ttgtcggtca ggaactcgcc
     2341 gcaggaaatg ggtctgcaag tcattcgcgg cttggctgag atgctggaaa gccctgctgg
     2401 tggactgtgg ctgcgcaaca agggagatgc cgcattcagc caaatggccc gctggaatca
     2461 ggcccaagcg tctgcgaaag aggacttgga ctccccgctg tgccagttca tgaaaaccgg
     2521 tggctgggtc atcaacctgg aagagttccg ttctttctca cgtcgctatg gcgaactcaa
     2581 gcttccttcc tggttgcagg aattgccgca ggcctggttg ctggtaccgc ttctggtggg
     2641 cgacgatctg atcgggtttg tggtgctagc cagcgccaga acccgggtgg acgtgaactg
     2701 ggaggtcaat gatctcctga agaccgcagg gcaacaggca gccagttatc tggcacaaat
     2761 gcaggcaacc gaggcgctgc tggaggtgcg caagtttgat gcgttcaacc gtatgtcggc
     2821 t
//
LOCUS       DAOSLL010000617         2809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_31127_length_2809_cov_4.432462, whole genome shotgun sequence.
ACCESSION   DAOSLL010000617 DAOSLL010000000
VERSION     DAOSLL010000617.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31127_length_2809_cov_4.432462"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1255)
                     /locus_tag="PLB25_19865"
     CDS             complement(<1..1255)
                     /locus_tag="PLB25_19865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HOE43861.1"
                     /translation="MFSFFKKKLGGPPSITPTAQAATAAQPGTPDAVPAVLPPPATSP
                     ATSPAGATVAPAGPSAPDRIATSRMRPAAAQPGLPEAVGLADSPTTSASVGTPAARQG
                     WMDKLKAGLRKTGSSIAGVFTGAQIDDALYEDLETALLMADTGVKATAHLLVDLKFRV
                     VDNGSTRPSEVKGLLVDGLAHMLSPLQKSLVIGQYAPTVIMVTGVNGAGKTTSIGKLT
                     RHLADSGASVLLAAADTFRAAAREQLGVWADRNTVDIISQDGGDPAAVSFDAVSAGKA
                     RGRDVVLIDTAGRLPTQLHLMEELKKIKRVIQKTGVVTDRALATGSANPLPGSGDPAA
                     SSLPPHEILLVIDGNTGQNALNQVKAFDDALGLTGLIVTKLDGTAKGGVLAAIARERP
                     IPVYFIGVGEKLEDLETFNAREFAQA"
     gene            1372..2754
                     /locus_tag="PLB25_19870"
     CDS             1372..2754
                     /locus_tag="PLB25_19870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010463123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HOE43862.1"
                     /translation="MALAPDPQVAPTSGNKSTSVGNAGVQQFTLANGMTLIVKPDRRA
                     PTAVHMLWVRVGSIDEVDGASGVAHVLEHMMFKGTPSLKAGEFSRRVAALGGRENAFT
                     SRDYTGYYQQIPAGKLADVMQLEADRFAHSQWPDDEFKKELEVVKEERRMRTEDNPRA
                     LLGEALNATAFVASPYRRPVIGWMSDLESLTPFDARDFYQRWYVPANAAVVVAGDVDV
                     AQVRVLAEQYYGSLPARAVPERKPRAEPKQVGLKRIAFKAPAEQANVTLAFKVPALQW
                     QEGAADDADALALTVLAAVLDGYSGARLERSLTQGPERVADSVGAHNGLWGRGPQLFM
                     LSGVPAPGKTSDQVEQGLRAQVQRIAQDGVTEAELARVKTQWVASEVYKLDSLFNQAR
                     ELGSQWVQGLLPDAGEQLIVKLRAVTAAQVKAVAGRYFGDDGLTVAQLLPQPPDKTRK
                     ARPTAAGLRH"
BASE COUNT          498 a          861 c          928 g          522 t
ORIGIN      
        1 aggcctgggc aaattcgcgg gcgttgaagg tttccaggtc ttcaagcttt tcgccaaccc
       61 cgatgaaata caccggaatc ggtcgttcac gggcaatcgc tgccagcacg ccgcctttgg
      121 cggtgccgtc aagtttggtg acgatcagcc cggtcaggcc aagcgcatcg tcaaaggcct
      181 tgacctggtt cagggcgttt tgaccggtgt tgccgtcaat caccagcaaa atttcatgcg
      241 gtggcagact gctggcagcc gggtcgccag agccgggcag ggggtttgct gacccggtgg
      301 caagcgcacg gtctgttacc acaccggtct tctggatcac ccgtttgatc tttttcagct
      361 cttccatcag gtgcagttgg gtcggcagcc gtccggcggt gtcgatcagc accacgtcac
      421 gtccgcgcgc cttgccggcg ctgaccgcgt caaacgaaac ggcagccggg tcgccaccgt
      481 cctggctgat gatatcgacc gtgttgcggt cggcccacac gccgagttgc tcgcgcgcgg
      541 cggcgcgaaa ggtgtcggct gccgccagca gcacgctggc accagaatcc gccagatgcc
      601 gggtgagttt gccaatcgag gtggtcttgc ccgcgccatt gaccccggtg accatgatca
      661 ccgtcggtgc atactggcca atcaccagcg acttttgcag cggactgagc atgtgggcga
      721 gcccatcgac cagcaagccc ttgacttccg atggccgggt gctgccgttg tcaaccaccc
      781 ggaacttcag gtccaccagc aaatgcgcgg tggccttgac cccggtgtcg gccatcagca
      841 gggcggtttc caggtcttcg tacagcgcgt cgtcaatttg cgcgccggtg aacacgccgg
      901 caatgctgct gccggtcttg cgtaaaccgg ccttgagttt gtccatccag ccttggcgcg
      961 cggctggcgt gccgaccgat gccgaagtgg tcgggctgtc ggccagtccg accgcttcgg
     1021 gcaagcctgg ttgcgcggca gcagggcgca tccgggatgt tgcgatgcgg tctggcgcac
     1081 tggggccagc gggtgcaacc gttgccccgg caggtgacgt ggcaggtgac gtggcagggg
     1141 ggggcagaac ggctggcact gcgtcaggtg tgcctggctg cgctgctgtc gcagcctgag
     1201 cggtcggcgt gatggagggg ggccccccga gctttttttt gaagaaactg aacatttttt
     1261 gaaatcgtgg tatcacagca ttctatgaaa caccacccat ccctcttgca acgcctgttt
     1321 tgcgctgccc gcctgcttgc tcccggcctg ctttggttcg ctgcttctgc catggcacta
     1381 gcgccagacc cccaggtggc accgacatca ggcaacaaat cgaccagcgt tggcaatgcc
     1441 ggggtgcagc aattcaccct ggccaatggc atgacgctga ttgtcaagcc agaccggcgg
     1501 gcaccaacgg cggtgcacat gctgtgggtg cgggtcggct cgatcgacga agtcgatggt
     1561 gcctcgggcg ttgcccatgt gcttgaacac atgatgttca agggcacgcc aagcctcaag
     1621 gctggtgaat tttcgcgccg cgtggcggcc ctgggtggcc gcgaaaacgc cttcaccagc
     1681 cgcgattaca ccggctacta ccagcagata ccggcgggca agctggccga tgtgatgcag
     1741 cttgaggccg accgttttgc ccacagccaa tggccggacg atgagttcaa aaaagaactc
     1801 gaagtggtca aggaagagcg ccgcatgcgc accgaggaca acccgcgcgc gttgcttggc
     1861 gaggcgctga acgccaccgc ctttgtcgcc tcaccctacc gtcggccagt tatcggctgg
     1921 atgagcgacc tggagtcgct gacccccttt gatgcgcgtg acttttacca gcgctggtat
     1981 gtgccagcca atgcggcggt ggttgtggcc ggtgacgttg atgtggcgca ggtgcgtgtg
     2041 ctggccgaac agtattacgg cagcctgccc gcgcgtgccg tgcctgagcg caagccgcgc
     2101 gccgagccta agcaggttgg cctcaaacgt atcgccttca aggccccggc cgagcaggcc
     2161 aatgtgacac tggccttcaa ggtgccggcg ctgcaatggc aagaaggcgc agcggacgat
     2221 gccgatgcgc tcgcgctgac ggtgctggcg gcggtgcttg acggctacag cggagcccgg
     2281 ctggagcgca gcctgacgca gggaccagag cgggtggccg acagcgtggg tgcccacaac
     2341 ggcctgtggg gtcgcgggcc gcagttgttc atgctcagcg gggtgccggc cccaggcaag
     2401 accagcgacc aggtcgagca gggtttgcgg gcgcaggtgc agcgcattgc gcaggacggt
     2461 gtcactgagg cagaactggc gcgtgtcaaa acccagtggg tcgccagcga ggtctataag
     2521 ctcgactcgt tgttcaatca ggcgcgtgaa ctcggcagcc agtgggtgca gggcttgctg
     2581 ccggacgcag gcgagcaact gatcgtgaag ctgcgcgccg tcaccgcggc ccaggtcaag
     2641 gcggtagcgg gccgctattt cggcgacgat gggctgaccg tggcgcaact gctgccgcaa
     2701 ccgcccgaca agactcgcaa agcgcgcccg acggccgcag gattgcgcca ttaatctcta
     2761 acaagtcaag tctttgcata ccatgcgaag aattcaaaca ttcaaagtc
//
LOCUS       DAOSLL010000618         2809 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_31132_length_2809_cov_3.123457, whole genome shotgun sequence.
ACCESSION   DAOSLL010000618 DAOSLL010000000
VERSION     DAOSLL010000618.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31132_length_2809_cov_3.123457"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="PLB25_19875"
     CDS             complement(<1..540)
                     /locus_tag="PLB25_19875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43863.1"
                     /translation="MQSLPKFFAKLLVVASMMFAVFAVAQSGPSMSEIYAKAQAGKLD
                     EAQVMVQQVLISHPNSAKAFFVQAELYSRQGKLDLARESLSTAEKYASGLPFVKPEAL
                     QTLRTQLASKPGLKSTSGLSSNFTSQQTQTSTSWLVPLLLTGGVIAAAFFFFRRRKVE
                     SCAQQSAYVQQNGLNGPQMF"
     gene            complement(587..763)
                     /locus_tag="PLB25_19880"
     CDS             complement(587..763)
                     /locus_tag="PLB25_19880"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOE43864.1"
                     /translation="MPAIPAGASCPSCNALNPNAARFCFQCGGSMQPARCTQCGTTLA
                     VGAKFCSQCGKQAV"
     gene            complement(715..888)
                     /locus_tag="PLB25_19885"
     CDS             complement(715..888)
                     /locus_tag="PLB25_19885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43865.1"
                     /translation="MTIYLDVTAVAMAVVTVVAMAIIMVVIIKALPVEVTGHQVHLCR
                     PFQLVQVALAAML"
     gene            complement(943..>2809)
                     /locus_tag="PLB25_19890"
     CDS             complement(943..>2809)
                     /locus_tag="PLB25_19890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013742902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HOE43866.1"
                     /translation="NFPSGIQKYALALLFAVGAESISFFAPDTRIFQGAGMVLSVVAI
                     ALAGFSTYVKGLAALRNFRLNINALMTVAVTGAFLIGQWPEAAMVMALYAIAELIEAR
                     AVDRARGAIKSLLDLTPETAEIRQLDGSWQTFPVNNVVLDAVVRVKPGARIPVDGVIA
                     IGSTAVDQASVTGESIPVDKVPGDAVFAGTINATATIEVRVTALPSNSVLSRIIHAVE
                     QAQGSRAPTQQFVDKFAAIYTPAVFVLALGVALLTPWLIDWTWVQAIYKALVLLVIAC
                     PCALVISTPVTVVSGLAAAARRGILIKGGVHLEGARKIKVVALDKTGTITEGKPVLVD
                     TEFVKSAIPEWQVLRWAADLSAHSDHPVSKAIAKSLDPENSVLQDFKVVAGRGVEANV
                     DGQPLILGNHRLIEERKLCNSSIEARLAEHEALGRTVTMLASQTQVLAIFAVADTIKE
                     TSREALAQLHALGVTTVMLTGDNEATAKAIAKQAGIDDARGNLLPEDKLTAIEEFQKR
                     YGMTAMTGDGINDAPALARSDIGFAMGVAGTDTAMEAADVVIMNDDLRRIPETILLSR
                     KTQAILWQNISLALGIKAVFLVLALFNGASMWMAVFADMGASLLVVGNGLRLLRK"
BASE COUNT          680 a          769 c          709 g          651 t
ORIGIN      
        1 gaacatctgc ggtccgttta atccattttg ttggacgtat gcagattgtt gagcgcacga
       61 ttccactttg cgtctgcgaa aaaagaaaaa agcagcggca atcacaccgc cggtgagaag
      121 cagagggacc agccaagatg tagaggtctg tgtttgttgc gacgtaaagt ttgaggacaa
      181 accagacgta gatttcagcc ctggtttgga cgcgagttga gtgcgcagtg tctgtaacgc
      241 ctcaggtttt acaaacggaa ggcctgacgc gtatttttca gcggtagata gtgactcccg
      301 agcgaggtct agcttgcctt gacgtgagta caactccgct tgaacaaaga atgctttggc
      361 gctgttggga tgcgagatca acacttgctg caccattact tgcgcttcat ccagttttcc
      421 tgcctgtgcc ttcgcataaa tttcgctcat actcggcccg gactgcgcca cggcgaaaac
      481 tgcaaacatc attgatgcca cgacgagcag ttttgcgaaa aatttgggta acgactgcat
      541 ttcagttcct ttttgaaagt gttgtcttgg tcagtgaatc tgtgttttaa acagcttgtt
      601 taccgcactg actacagaat ttggctccca cagccagggt agtcccacat tgagtgcacc
      661 gcgccggttg cattgagccc ccgcattgaa agcagaatct tgccgcgttc gggttcaaag
      721 cattgcagct agggcaactt gcaccagctg gaatggccgg cataggtgga cttgatggcc
      781 cgtaacttcc accgggaggg ccttgataat gaccaccatg atgatcgcca tggccaccac
      841 cgtgaccacc gccatggcca ccgccgtgac gtccaaatag attgtcaaaa atgctcatga
      901 tttcacgctt tcattcaata ataaatcctg ccgccttggg ggttacttcc tcagtaaacg
      961 cagaccgttc ccaacaacca atagactcgc ccccatatcc gcaaacaccg ccatccacat
     1021 ggatgcacca ttgaacaatg ccagcaccag aaatacagcc ttgatgccca gcgccaaaga
     1081 aatgttctgc caaaggatgg cttgcgtttt gcgtgatagc aagatggtct cggggatacg
     1141 ccgcaagtca tcgttcatga tgaccacatc ggcggcttcc atggcggtat ccgtgcctgc
     1201 aacacccatg gcaaatccaa tatcggaacg cgccaatgca ggcgcatcgt tgatgccgtc
     1261 gccggtcatt gcagtcatac cgtagcgttt ttgaaattct tcgattgcag tcagcttgtc
     1321 ttctgggagc aaattcccac gtgcatcatc aatgccagcc tgcttggcaa tagcctttgc
     1381 ggtggcttcg ttgtcacccg tcaacatgac ggtggtgaca cccagggcgt gcaattgggc
     1441 cagtgcctct cgcgaagttt ctttaatggt gtcggctact gcaaagatgg ccaacacctg
     1501 ggtttgcgat gccagcatgg tgacggtgcg acccagcgct tcatgctccg ccaatcgcgc
     1561 ttcgatactg ctgttacata atttccgctc ttcaatcaag cggtgattgc cgagtatcag
     1621 cggctgtcca tcaacgttgg cttctacgcc tcttccagcg actaccttga agtcctggag
     1681 aacactattt tcaggatcga ggcttttcgc gatggcttta gacaccgggt gatcagagtg
     1741 agcagaaagg tcggcggccc agcgcaaaac ttgccactca ggtattgctg atttaacaaa
     1801 ttcggtatcc accaaaacag gcttgccctc ggtgattgtt cccgtcttgt ccagagcaac
     1861 tactttgatc tttcgggcac cttccaggtg aacaccaccc ttaattagaa tgccacgcct
     1921 tgccgccgca gccaggccac tgaccactgt gactggcgta gaaatcacca aagcgcaagg
     1981 acacgcaatc accagcagaa ccaacgcttt gtagatggct tgaacccaag tccaatcgat
     2041 gagccatggc gtgagcaatg cgactcccag agccaggacg aacacagcgg gtgtgtagat
     2101 ggcggcaaac ttgtcaacaa actgctgagt gggtgcacgg gaaccctgcg cttgttcaac
     2161 tgcatggatg atgcgagaaa gcactgagtt acttggcaag gcggttacgc gcacttcaat
     2221 ggtggctgtg gcgttgatcg tcccggcaaa tacggcatca cctggaacct tgtctaccgg
     2281 aatgctctca ccggtcaccg acgcttggtc tacggcagtg ctgccaatgg caataactcc
     2341 gtccacagga atacgcgcac ccggtttgac gcgtacaacg gcatccagta cgacattatt
     2401 gacagggaaa gtttgccaag atccgtcaag ctgacgaatc tcagcggttt caggggtcag
     2461 atccagcagg ctcttgatgg cccctctggc ccggtccact gcacgcgctt caatcaattc
     2521 agcgatggca tacagcgcca tgaccattgc cgcctcaggc cactgaccaa tcagaaatgc
     2581 ccctgtaacc gcgacggtca ttaatgcgtt gatattgagt ctgaaatttc gcagggctgc
     2641 caagcctttg acataggtgg agaagcctgc cagcgcaatt gccactaccg atagcaccat
     2701 ccccgcacct tgaaaaatgc gtgtgtcagg ggcaaaaaat gaaatggatt ccgctcccac
     2761 tgcaaaaagt aaagcgagcg catatttttg aatcccactg ggaaaattg
//
LOCUS       DAOSLL010000619         2808 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_31144_length_2808_cov_3.732655, whole genome shotgun sequence.
ACCESSION   DAOSLL010000619 DAOSLL010000000
VERSION     DAOSLL010000619.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31144_length_2808_cov_3.732655"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            528..2588
                     /gene="mutL"
                     /locus_tag="PLB25_19895"
     CDS             528..2588
                     /gene="mutL"
                     /locus_tag="PLB25_19895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HOE43867.1"
                     /translation="MNPALPQPLPRRPIRELPDELISQIAAGEVVERPASVVRELLDN
                     AVDAGAGQITVRLLAGGVRLISVEDDGTGILPDELPIALKRHATSKIGSLLELESVGT
                     MGFRGEALAAINSIADCTILSRATGQNHAYLLEGHSGELKPVARADGTTVEVKELFFS
                     TPARRKFLKTDATELAHCVDAVRRHALARPDIGFAVWHEGKLAEQWRRCGELASLPAL
                     EQRLADVLGSTFVEQSVWVDHASSTRPGQPGHAGLIKVWGRAGIPDIARARGDLQFCY
                     VNGRFVRDKVISHAARSAYEDVLHGQRQPVYVLYIELDPTRVDVNVHPTKIEVRFRDS
                     REVHQAVRHAIESALAVSRAGTTAAVALNLSEAVASTANHGANWPPAHSYTAQSAINF
                     AAKQGPTGHQVSDLAALWGQPQRQGGQSTHPAGFAQAQPDNAWLQPSATQPQGEPHSE
                     PTPARATQGDADNASAWPLGRALAQLQGIYILAENAQGLVIVDMHAAHERIVYERLKA
                     QSHAVAQPGTGSARAGVPEAARPTASPAIEHRALPSQPLLIAATFAATPVEIATAEAH
                     ADTLHALGLEITPFSARTLAVRAVPVALTHGDAVELARGVLAELSGHDASSVIARAHH
                     ELLSTMACHGAVRANRRLTLEEMNALLRQMETTERSDQCNHGRPTWRQISVRELDALF
                     MRGR"
BASE COUNT          565 a          891 c          869 g          483 t
ORIGIN      
        1 gagcggcaac tgttttgacg aaagttcagt tgcacaggtt caagcacgca aggcaatgca
       61 ctttttagcc gattcatgca attgtctgag tggcggaaag tcgtctacat cgcgagcagc
      121 cacctcgaat gtcataaccc atctggccag accaacggtg acttgccgag ttgcaggcga
      181 gcgactttca aagcccggct atccagaccg acacccgctc cctgcgtttg gactatctgc
      241 tgctgccaga ccggcggctg caaaattggc agccgcattg ccattcatgg ggtcgatgcg
      301 caaaacttca ggcatttgaa actgttgacc gatgccatcg tgcgcaagtt tgcgccactg
      361 gccgacacca agcttgcttc gacgtgcagc gtacagccat gctggcgttg cctcgatgcg
      421 gtggcccggc agcggttgcg ctgaatctgg gcgaaactgc ggcaaccgct gccagtcctg
      481 acacaattca tgctactgcc catagctatt cagccgagca atccaggatg aacccagccc
      541 ttccacagcc cctgccccgc cgcccgatcc gcgagttgcc agacgaactg atcagccaga
      601 tcgcagcggg cgaagtggtg gagcgcccgg cctcggtggt gcgcgaactg cttgacaacg
      661 cggtcgatgc cggtgcgggg caaatcaccg tgcgtctgct ggctggcggg gtgcggctga
      721 tcagcgtgga agacgatggc accggcatcc tgcccgacga gttgccgatt gccctcaagc
      781 gccatgccac cagcaaaatt ggcagcctgc tggagctgga atcggttggc accatgggtt
      841 ttcgggggga agccctggct gcaattaatt cgatagctga ctgcacaata ctgtcaaggg
      901 ctacaggcca aaatcatgcc tatcttctgg aaggccatag cggtgaactc aaaccggtgg
      961 cccgcgccga cggcaccacg gtggaggtca aggaactgtt tttttcaacc ccggcgcgcc
     1021 gcaagttcct gaaaaccgat gccaccgagc tggcgcactg cgtcgatgcg gtgcgccgcc
     1081 acgctctggc ccgccctgac atcggctttg ccgtctggca tgagggcaaa ctggccgaac
     1141 aatggcggcg ctgcggcgag ctcgccagcc tgccggccct ggagcagcgt ctggccgatg
     1201 tattgggcag cacgtttgtc gagcagtcgg tgtgggtgga ccatgccagc agcacccggc
     1261 cgggccagcc cggacacgct ggcctgatca aggtttgggg ccgtgccggc attccggata
     1321 ttgcgcgggc ccgaggcgac ttgcagtttt gttatgtcaa cggccgcttt gtgcgcgaca
     1381 aggtcatcag ccatgccgcc cgcagcgcct acgaagacgt gctacacggc cagcgccagc
     1441 cggtgtatgt gctgtacatc gaacttgacc cgacccgggt cgatgtgaat gtgcacccga
     1501 ccaagatcga ggtgcgtttt cgcgacagcc gcgaggtcca tcaggcggtg cgccacgcca
     1561 tcgaaagtgc gctggcggtg tcacgggcgg gcaccacggc ggcggttgcg ctgaacctga
     1621 gcgaagccgt ggcaagcact gccaatcatg gcgcgaattg gccgccagcc catagctaca
     1681 ctgcgcaatc agctatcaat ttcgctgcaa agcaggggcc aactggccac caggtcagtg
     1741 atctggcggc actgtggggg cagccgcagc ggcaaggcgg ccagagcacc cacccggctg
     1801 gtttcgccca ggcccagcct gacaatgcct ggctacagcc gtctgccacc caaccgcagg
     1861 gcgagccaca cagcgagcca acaccagcca gagcgaccca aggcgacgct gacaacgcca
     1921 gtgcctggcc actgggccgg gcgctggcgc aattgcaggg catctacatc ctggccgaaa
     1981 acgctcaggg tctggtgatt gtcgacatgc acgcggccca cgaacgcatt gtttacgagc
     2041 ggctcaaggc gcagtcgcat gcagtcgcgc agccgggcac cgggtcagcc cgcgctggcg
     2101 tacccgaggc tgcccgaccg acagcttcac cggccattga acaccgggcg ctgcccagcc
     2161 agccactgct gattgccgcc acctttgccg ccaccccggt tgaaatcgcc accgccgagg
     2221 cacacgctga cacgctgcac gcgctggggc tggaaatcac acccttttcc gccagaacgc
     2281 tggcggtacg ggccgtgccg gtcgcgctga cacatggcga tgcggttgaa ctggcgcgcg
     2341 gtgtgctggc cgaactcagc gggcacgatg ccagcagcgt gattgcccgt gcccaccatg
     2401 agttgctgtc caccatggcc tgccacggtg ccgtgcgcgc caaccgccgt ctgacgctgg
     2461 aagagatgaa cgccctgctg cgccagatgg agaccaccga gcgtagcgac cagtgcaacc
     2521 acggccgccc gacctggcgg caaatcagcg tgcgagagct cgatgcgctg ttcatgcgcg
     2581 ggcgctgaca gcaggtaccg ttaccggctg cgcacaaagt ggtactcaaa ttgaactacc
     2641 gtttgattgc atttccagat cattcgtgtc caggtgcctt ggcgcagaca tggcaaacgc
     2701 aaggcaaggc gagataaaga ggacatcgac gatttggaaa aggaatgagt gccatagtaa
     2761 ccatcccccg gcaaagccgg gggctttaag aattgtgagc cgctaaaa
//
LOCUS       DAOSLL010000620         2802 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_31234_length_2802_cov_4.520568, whole genome shotgun sequence.
ACCESSION   DAOSLL010000620 DAOSLL010000000
VERSION     DAOSLL010000620.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2802
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31234_length_2802_cov_4.520568"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="PLB25_19900"
     CDS             complement(<1..445)
                     /locus_tag="PLB25_19900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43868.1"
                     /translation="MSTITLTTTQHAMLDHAIHHTDGRVDWFPENIKGGARQKVLQGL
                     FNRALITPHGSDWLVAAEGYDALGCARPNACSIAPDPELDAAVAQAEATWAQGVDTNE
                     SASAAQAESGEVAIETETQVDQHDNVTGCDERLAGTDAPATEIDAT"
     gene            complement(541..750)
                     /locus_tag="PLB25_19905"
     CDS             complement(541..750)
                     /locus_tag="PLB25_19905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015914460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43869.1"
                     /translation="MKSTIDHIFALIAEKHLFIETLETRNSDSLDFHDVSVWGVRSAL
                     EAAFKAGVELGASMPKPTESEIGNT"
     gene            complement(933..1199)
                     /locus_tag="PLB25_19910"
     CDS             complement(933..1199)
                     /locus_tag="PLB25_19910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43870.1"
                     /translation="MNNTAAPSTTQVAEQLRDIQLELIDLLDRAKELLRTAPSITRER
                     AESYWLAHARMAITKNHSYLGGSMVDMDDTIAEMEGADGEDDES"
     gene            1463..2017
                     /locus_tag="PLB25_19915"
     CDS             1463..2017
                     /locus_tag="PLB25_19915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elements of external origin"
                     /protein_id="HOE43871.1"
                     /translation="MGLSIRAYARHRGVTDTAVHKAIRSGRINALADGTIDPDQADAQ
                     WERNTSSPKTGTQRPTVKVKVPEVDGDGERSGAGAATNTGSGGGGTSLLQARTVNEVV
                     KAQTNKVRLARLKGELIDRPQAIAHVFKLARSERDAWLNWPARVSAQMAAKLELDAHT
                     MHVALENAVREHLQELGNLQASVD"
     gene            2017..>2802
                     /locus_tag="PLB25_19920"
     CDS             2017..>2802
                     /locus_tag="PLB25_19920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009521790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HOE43872.1"
                     /translation="MSTQIDTDHYDGATEIERAWRDGLTPDPLLSVSEWSDQHRMLSS
                     KASAEPGRWRTSRTPYLKEIMDCLSPNSPVERVVFMKAAQLGATEMGSNWIGYVIHHA
                     PGPMMAVWPTVDMAKRNSKQRIDPLIEESAALRELISPARSRDSGNTILAKEFRGGVL
                     VMTGANSAVGLRSMPVRYLFLDEVDGYPLDVEGEGDAISLAEARTRTFTRRKIFIVST
                     PTIAGISAIEREYEASDQRRYFVPCPHCDHRQWLRFEQLRWEKA"
BASE COUNT          544 a          758 c          833 g          667 t
ORIGIN      
        1 gtgtcgcgtc gatttcagtt gctggtgcat cagtgccagc caggcgttcg tcgcagcctg
       61 tgacgttgtc gtgctggtcg acttgtgttt cggtttcgat tgcgacttcg cctgattcgg
      121 cttgagctgc tgacgctgac tcgttggtat ccacaccttg cgcccacgtt gcctcagcct
      181 gcgccacggc tgcgtcaagt tctgggtctg gtgcgatgct gcaagcattg ggtctggcgc
      241 accccagcgc gtcgtagccc tcggcggcga ccagccagtc tgacccatgt ggggtgatca
      301 aggcgcgatt gaaaagtcct tgcaaaacct tctggcgtgc gccgcctttg atgttttctg
      361 gaaaccagtc aacgcggcca tcggtgtgat ggatggcgtg gtccaacatc gcgtgctggg
      421 tggtggtcag tgtgatggtg ctcatttagg gctccttttt ggtggttgtt aagacgatgt
      481 gatgaacgcg ctgtttgcaa gacaagccaa gctttttctg cttggcttgt cgtggtttgt
      541 ttatgtgttg ccaatctccg actcagtcgg ttttggcatg gatgcaccca actcaacccc
      601 ggctttaaag gctgcttcca aggcgctgcg cacaccccag actgacacgt catggaagtc
      661 aaggctgtct gagttgcgtg tttccagggt ctcgataaag aggtgcttct cagcaatcag
      721 ggcaaaaata tggtcgatgg tgcttttcat ttcaatctcc gtttggttgg ttgcgatgct
      781 tgtagtaaga cgctgtgtgc ttgagaagcc aagtcaattt cgaacatttt ttgattcatt
      841 tttttgagac agtcgcttta atctgacgct gctttgaatg cctgcatgaa ggcctgcgcg
      901 ccgccagttt tgttggcggc gctgcgtttt gatcaggact cgtcgtcctc gccatctgcg
      961 ccttccatct ctgcgatggt gtcgtccatg tcaaccatgg agccacccaa gtaactgtgg
     1021 tttttggtga tggccatgcg ggcgtgtgcc agccagtagc tctctgcgcg ttcgcgggtg
     1081 atgcttgggg cggtgcgaag aagttctttg gcgcggtcaa gtaagtcgat caattcaagc
     1141 tgtatgtctc gaagttgctc ggccacttgc gtggtggatg gggctgctgt gttattcatg
     1201 atgtgcgtcc ttttggttgg gtttcgtcgc gttgagcagg tttgctgcaa cacctctatg
     1261 aagacgctgt ttgcttgtga agccaagtca attcgcatca tttttttaaa tcttttgaat
     1321 caccttttgc ttcaatgcca caccccaatt tccgggcaaa tttgctggaa gttgattgaa
     1381 aacaatcatg caaatttgca tcttttttat gcccgcatcc gaggcccggc agaccttgca
     1441 atgaagaaca cacgcaccac ccatgggact atcgattcgc gcctacgccc gtcatcgcgg
     1501 ggtcaccgac accgccgtac acaaggccat ccgtagcggg cgcattaatg cactggctga
     1561 cggcacgatt gatcccgatc aggcggatgc ccagtgggag cgcaacacca gttcgcccaa
     1621 gacgggaaca caacgaccga ccgtcaaggt caaggtgccg gaggtcgatg gcgacggtga
     1681 acgaagtggt gcaggcgctg caactaacac tggctctggc ggcggtggca cctcgctctt
     1741 acaagcccga accgtcaacg aggtggttaa ggcgcagacc aacaaagtgc gactggccag
     1801 gcttaagggc gaactcattg atcggccgca ggccattgcc catgtgttca agctggcgcg
     1861 cagtgaacgt gatgcgtggt tgaactggcc tgcgcgggtt tctgcccaga tggccgccaa
     1921 attggaattg gatgcgcaca ccatgcacgt ggcgctggag aacgcggtgc gcgagcacct
     1981 acaagaactg ggcaatttac aggcaagcgt tgactgatga gcactcaaat cgataccgat
     2041 cattacgacg gcgcaacaga gattgagcgt gcgtggcgcg acggccttac cccagaccct
     2101 ctgctgtcgg tgtccgagtg gtcagaccag caccgcatgc tctccagcaa ggcatcggcc
     2161 gagccagggc gctggcgcac cagccgcacg ccgtacctca aggaaatcat ggactgcctg
     2221 tcacccaatt cgccggtgga gcgggtggtg ttcatgaagg cggcgcagct cggtgccacc
     2281 gagatgggca gtaactggat tggctacgtg atccaccatg cgcccgggcc catgatggcg
     2341 gtctggccga cggtggacat ggccaagcga aattccaagc agcggatcga ccctctgatt
     2401 gaggagtccg ctgcattgcg tgaattgata tcaccagccc ggtcgcgcga ctccggcaac
     2461 accatcctgg ccaaagagtt caggggcggc gtactggtga tgaccggtgc caacagcgcg
     2521 gtgggcttgc gctcgatgcc ggtgcgctat ttgttcctgg acgaggtcga cggttacccg
     2581 ctggacgttg aaggcgaagg tgatgcgatt tcgctggccg aagcacgcac gcgcaccttc
     2641 acacggcgca agatatttat cgtgtcgact ccgaccatcg cgggtatttc ggccattgag
     2701 cgcgaatacg aggcaagtga ccagcggcgc tactttgttc cctgtccaca ctgcgatcac
     2761 cgccagtggc tacgttttga gcaactccgc tgggaaaaag cc
//
LOCUS       DAOSLL010000621         2797 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_31296_length_2797_cov_4.632020, whole genome shotgun sequence.
ACCESSION   DAOSLL010000621 DAOSLL010000000
VERSION     DAOSLL010000621.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31296_length_2797_cov_4.632020"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            135..401
                     /locus_tag="PLB25_19925"
     CDS             135..401
                     /locus_tag="PLB25_19925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prevent-host-death protein"
                     /protein_id="HOE43873.1"
                     /translation="MQVTATQAKNRSGFFSAKANGEPVFIEKDGQLDSVILSFDTFQT
                     LKAAQAPVSFEQRKREFQEQHKNWIAAQNVHRDAYGLWCGGSAA"
     gene            416..733
                     /locus_tag="PLB25_19930"
     CDS             416..733
                     /locus_tag="PLB25_19930"
                     /inference="COORDINATES: protein motif:HMM:NF013965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HOE43874.1"
                     /translation="MAQHDVYPNPQIESRPFMPYVVDVQSNLIDCLSTRLMVPLSRVG
                     ARLPKLPVDLSPVLSVDGESLSLQPHLSAPVVTSSVRYPAMNLQRHASDIAAAIVSVV
                     CGV"
     gene            726..1214
                     /gene="nudB"
                     /locus_tag="PLB25_19935"
     CDS             726..1214
                     /gene="nudB"
                     /locus_tag="PLB25_19935"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007857779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HOE43875.1"
                     /translation="MSDALAAFKIPQSVLVVIYTPTLDVLLIQRADADDFWQSVAGSK
                     DTPDESYEDTARREVFEETGIDCTTGSSLAANLQDWHLENIYPIYPRWQHRYASGVLL
                     NTEHLFGLLVPTGIGVRLSPREHTAFQWLPYQQAADVCFSPSNAEACLMLPRFAGMTA
                     PP"
     gene            1211..1945
                     /locus_tag="PLB25_19940"
     CDS             1211..1945
                     /locus_tag="PLB25_19940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HOE43876.1"
                     /translation="MSTIRVATYNIHKGVQGLGPTRRLEIHNLALAVEALDADVVCLQ
                     EVRQSNLREAKHFDHWPAQCQADYLAPLGYEAVYRTNAVTRHGEHGNALLSRWPIVGH
                     QHEDMSDHPLEQRGLLHVELMLHGRCVHVLVVHLGLIKASRIRQVAQLKRFVTREIPL
                     DAPLLVAGDFNDWSNRVQEAFAGAGLFAMGGMAQPTFPSRLPLAQLDHVFARGLRPVS
                     LSVPRGRVWWRMSDHLPLIAEFELPS"
     gene            1956..>2797
                     /locus_tag="PLB25_19945"
     CDS             1956..>2797
                     /locus_tag="PLB25_19945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HOE43877.1"
                     /translation="MAELKAGHQLHLLKGSKEFFEALVAAMDASLHEVRLETYIFRTQ
                     ATGEQIAQALVRAAHRGVTVCVVMDGVGTPALPSPWPQRFDDAGVQWRIYSPLGRLGL
                     LLPSRWRRLHRKLCVVDGQVAFCGGINVLDDWFDPNHGQLDAPRFDFAVQVYGPLVQQ
                     AHLAMVQFWWRLQAVQSVNQVDWKGAWHALQQAVQEKRSAEVGQVDPGSAMAALVLRD
                     NLRYRTHIERAYRQALGEARHDIILANAYFLPGRKIRKSLVHAAQRGVRVRLLLQGRY
                     EYFM"
BASE COUNT          547 a          780 c          859 g          611 t
ORIGIN      
        1 ttggtgaaag ccgggtaagt ttgcagattc tgacttgatg caaaccggag gggcgctcac
       61 ttgagcgccc tcaatgtttg gggatgtcat caacttcaat ggctaaaata gccagcacag
      121 ccacgagaca tctgatgcaa gttaccgcca cccaagccaa aaaccgctcg gggttttttt
      181 ccgccaaagc caatggcgag ccagttttta tcgaaaaaga tggccaactc gacagtgtga
      241 tcctctcgtt tgacaccttc cagacgctca aggcggccca ggcaccggtc agttttgagc
      301 agcgcaagcg cgaatttcag gaacaacaca agaactggat tgctgcccaa aatgtccatc
      361 gcgatgcgta tgggctttgg tgcggcggtt cagcggcctg accagaagat gcgtgatggc
      421 gcagcacgat gtgtatccca atccacagat tgaatcgcgc ccattcatgc cttatgtggt
      481 cgatgtgcaa agcaatttga ttgactgcct gagcacccgc ctgatggtgc cgctgtcgcg
      541 tgtcggtgcg cggctaccaa aattgccggt cgatttgtcg ccggtgctaa gcgtcgacgg
      601 cgaatccttg agcttgcagc cccatctgtc agcgccggtg gtgaccagct cggtcaggta
      661 cccggcgatg aacttgcagc gccacgccag cgacatcgct gctgccattg tttcagtggt
      721 ctgcggtgtc tgacgcgctt gctgccttca agatcccgca gtcggtgctg gtggtgatct
      781 acaccccgac gctcgacgta ttgctgattc agcgcgccga tgcggatgat ttctggcagt
      841 cggtggccgg cagcaaagat accccagacg agtcgtatga agacacggcc agacgcgagg
      901 tgtttgagga aaccggcatt gactgcacaa ctggctcaag cctggcggcc aatctgcagg
      961 attggcatct ggaaaacatt tacccaattt acccacgctg gcaacatcgc tatgccagcg
     1021 gcgtattgct caacaccgaa catctttttg gcttgctagt gcccaccggc atcggggtgc
     1081 gtctgagtcc gcgtgaacac acagcttttc agtggttgcc ctatcaacag gcggctgatg
     1141 tctgcttttc accgtccaac gccgaggcct gtttgatgct gccccggttt gccgggatga
     1201 cggcgccgcc atgagcacca tccgggtggc tacctacaac attcacaagg gcgttcaggg
     1261 cctcgggccg acgcgccgac tggagattca caatctggcg ctggcagtgg aagcgcttga
     1321 tgccgatgtg gtttgcctgc aggaggtgcg acaaagcaat ttgcgtgagg caaagcactt
     1381 tgaccactgg ccagcccaat gccaggctga ctatctggca ccgctgggtt atgaggcggt
     1441 ctatcgcacc aatgccgtaa cacgccatgg cgagcacggc aatgcgctgc tgtcgcgctg
     1501 gccgattgtg gggcatcagc atgaagacat gtccgatcac ccgctggaac aacgcggttt
     1561 attgcatgtg gagctgatgc tgcatggccg ttgcgtgcat gttctggtgg tgcatctggg
     1621 cttgatcaag gccagccgga ttcgacaagt ggcgcaactc aagcgctttg tgacgcgtga
     1681 gattccgctg gacgcgccgt tactggtggc gggtgatttc aacgactgga gcaaccgggt
     1741 acaagaggca tttgcgggtg ctggtttgtt cgccatgggc ggcatggctc agccgacctt
     1801 cccctcgcgc ctgccgctgg cgcaactcga tcacgtgttt gcccgtggtc tgcgaccagt
     1861 tagcctgtcg gtaccgcgtg gacgggtctg gtggcgcatg tcagaccatt tgccgctgat
     1921 tgccgaattc gaattgccgt cctgaggcaa ccagcatggc agaactcaag gcaggccacc
     1981 agttgcattt attgaaaggc tcaaaggagt tttttgaagc tctggtagct gcaatggatg
     2041 ccagcttgca tgaagtccgg ctggaaacct atatttttcg gacgcaggcc accggtgagc
     2101 agattgcgca ggcgctggtg cgtgccgcac accggggcgt cacggtttgc gtggtaatgg
     2161 atggtgttgg cacgccggcc ttgccatccc catggccgca gcggtttgac gatgccgggg
     2221 tgcagtggcg tatttactcc ccgttgggac gcctcggtct gctgttgccc agccgctggc
     2281 ggcgcttgca ccgcaaactg tgcgtggttg acgggcaggt ggcattttgt ggcggcatca
     2341 acgtgctgga cgactggttt gacccgaacc atggccaact cgatgcaccc cggtttgatt
     2401 tcgcggtgca ggtttacggc ccgctggtgc agcaggccca tttggccatg gtccagttct
     2461 ggtggcgttt gcaggctgtg caaagtgtta atcaggtgga ctggaaaggt gcctggcatg
     2521 ccctgcagca ggcggtacaa gaaaaacgca gcgccgaagt gggccaagtc gatccgggta
     2581 gcgccatggc agcactggtg ttgcgcgaca acttgcgcta tcgcacccat atagagcgag
     2641 cctaccgcca ggcgctgggc gaggcacgcc acgacatcat tctggccaat gcgtacttcc
     2701 tgcccggccg caagatccgc aaaagcctgg tccatgctgc gcagcgtggt gtgcgggtgc
     2761 ggctgctatt gcaagggcga tacgagtatt tcatgca
//
LOCUS       DAOSLL010000622         2788 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_31419_length_2788_cov_4.343944, whole genome shotgun sequence.
ACCESSION   DAOSLL010000622 DAOSLL010000000
VERSION     DAOSLL010000622.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2788)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2788
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31419_length_2788_cov_4.343944"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /gene="fliF"
                     /locus_tag="PLB25_19950"
     CDS             complement(<1..905)
                     /gene="fliF"
                     /locus_tag="PLB25_19950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HOE43878.1"
                     /translation="MPAATTVTAIPVNPTLGQRLASLDRAQRIRLGVGIVLFLAIGIV
                     GLVMGRQAEWRVLYANLADKDGGAIVAQLTQMNVPYQHAAGGGAILVPANMVYDTRLK
                     LASLGLPKGAVTGFELMDTANRFGMTQFQERLTFQRGLEGELIRSIQALSSVHSARVH
                     LALPNQNGFFRDQQKPSASVLLSLNAGRTLDRGQLAGIIHLVSSSVPEMDPKAVSVLD
                     DSGKLLSTPPEGENGAGGDAQQLQHVQQIEQLYSSRILDILEPVVGRQNVRAQVTAEL
                     DFSQTESTSELFKPNQAPDATAIRS"
     gene            complement(1140..1742)
                     /locus_tag="PLB25_19955"
     CDS             complement(1140..1742)
                     /locus_tag="PLB25_19955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HOE43879.1"
                     /translation="MNYTFVSATILLILITDPIGNIPIFANALKPVAPQRRARVILRE
                     VSIAFVLLLTFMFIGEGFLRVMNLSELSLQIGGGVILFLIALRMIFPPLKMESVEPQG
                     EPLIVPLAIPAIAGPSALATVLLLVSQAPEKRLEWIAALCVTMTVSAVVLVLAERIQR
                     IAGDRFVVALERLMGLVLVAVSIEMLLRGIKTFIRQIAGA"
     gene            complement(1739..2152)
                     /locus_tag="PLB25_19960"
     CDS             complement(1739..2152)
                     /locus_tag="PLB25_19960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HOE43880.1"
                     /translation="MNSLFHLAFNVTDLDQTRQFYGGVLGCREGRSTATWVDFDFFGH
                     QLSMHLGDLFKTAATGQVGEHLVPMPHFGVILKLPDWLALAERLTGANIDFILAPQQR
                     FAGQPGEQWTMFFRDPFGNPIEIKGFASLETVYTT"
     gene            2248..2613
                     /locus_tag="PLB25_19965"
     CDS             2248..2613
                     /locus_tag="PLB25_19965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43881.1"
                     /translation="MDFLHTLRNTPWLARLALLWFALTLGVAVASPMVNPQEELVICT
                     SAGMVKLKFNADGSVSSTISAQAQCPLCVVGGAPPGFVRLVIAPVPAPGLVLQGIAAT
                     PIAVASAAPPPSRGPPSPL"
BASE COUNT          618 a          864 c          788 g          518 t
ORIGIN      
        1 tggctgcgaa tggccgttgc atccggcgcc tgattgggct tgaacagctc ggaagtggac
       61 tcggtttgcg aaaaatcgag ctcggccgtc acctgggcac gcacattctg tcgccccact
      121 acaggctcca gaatgtccag aatacggctg ctgtagagct gctcgatctg ctgcacgtgc
      181 tgcagttgct gcgcatcacc acccgcgcca ttttctcctt ctggcggcgt ggacagcagc
      241 ttgccggagt catcaagcac gctaaccgcc ttagggtcca tctccggcac gcttgaggac
      301 accagatgaa tgatgcctgc aagttgcccg cgatcaagcg tacgcccggc attcaaactc
      361 aacagcactg aagctgatgg cttttgctga tcccgaaaaa acccgttctg atttggcaag
      421 gccagatgca cacgggcact gtgcaccgat gacaaggcct gaatcgagcg aatcaattca
      481 ccttcaagcc cgcgctgaaa ggtcaggcgc tcctgaaact gggtcatgcc aaagcggttg
      541 gcggtgtcca tcaactcgaa cccggtcacc gcgcccttgg gcaagcccag ggatgccagc
      601 ttgagccggg tgtcatagac catattggca ggcaccagga ttgcgccgcc gcctgcggca
      661 tgctggtaag gcacattcat ttgcgtcagt tgggcgacga tggcaccacc gtccttgtca
      721 gccagattgg catacagcac acgccactcg gcctggcgtc ccattaccag accgacgatg
      781 ccgatggcca aaaacagcac aatgccaacg cccaggcgaa tccgctgggc tcggtccaga
      841 ctggccaggc gttgacccaa agtggggttg acaggaatgg cagtgacggt agtggcggca
      901 ggcatgaaaa gcttccaggg caaaagtgca caagacacct tgaatgacgg tgattatttg
      961 gcccgtgcct gaaaacatta gcttgaaaag atcagctttt accggctact tcactgccat
     1021 gttggtgccg tggccgcgcc agcaatcggt cacgccgcca atttggctgt accgcaccat
     1081 gagttcacca ggaccagtgc cgcaaacccg gccaatcgcg cccggcgcgg gcgcaccgat
     1141 caggccccgg caatttgccg gataaacgtc ttgatgccgc gcaacagcat ctcgatcgac
     1201 acggccacca gtaccaggcc catcagccgc tccagcgcca ccacaaagcg gtcaccggcg
     1261 atgcgctgaa tgcgctcggc caacaccagc accactgcgc tgacggtcat cgtgacgcac
     1321 agcgcggcaa tccattccag ccgcttttcc ggcgcctgtg ataccagcaa cagcactgtg
     1381 gccagggcag atggcccggc aatcgcggga atggccagcg ggacaatcag cggctcgcct
     1441 tgcggctcga cgctttccat cttgagcggc ggaaaaatca tgcgcaacgc aatcaagaac
     1501 aaaatcacgc cgccaccgat ttgcaaagac agctcgctca aattcatgac gcgcaaaaaa
     1561 ccctcgccaa tgaacatgaa ggtcagcagc aacacaaagg caatcgacac ctcgcgcagg
     1621 atgacacggg cacgccgctg tggggccaca ggcttcaggg cattggcaaa aatcggaata
     1681 ttgccaatcg ggtcggtgat caggatcagc agaatcgtcg ccgagacaaa ggtgtaattc
     1741 atgtcgtgta caccgtctca agcgacgcaa aacccttgat ctcaatcggg ttgccaaagg
     1801 ggtcgcggaa gaacatggtc cactgctcgc ctggctggcc ggcaaaacgc tgttgtggcg
     1861 ccaagatgaa atcgatgttg gcaccagtca gccgctcggc cagcgccagc cagtcgggca
     1921 atttcagaat caccccgaag tgcggcatgg gcaccaggtg ctcaccgacc tggccggtgg
     1981 cagcggtctt gaaaagatcg cccaggtgca tcgagagctg atgcccaaaa aaatcgaaat
     2041 cgacccaggt cgcggtgctg cgcccctcgc ggcagcccag cacgccgcca taaaactggc
     2101 gcgtctggtc caggtcggtg acattgaagg ccaggtgaaa caagctgttc atggttgaca
     2161 ggatagcagg ccgaccaccc gcctgcgctg gctcaaggtt gatctgttga tctctgttgc
     2221 gccaagtcaa tgccataatc gcgccccatg gactttttgc acaccctgcg caacacaccc
     2281 tggctggcca gattggccct gctgtggttt gccttgacgc tcggtgtggc agtcgcctcg
     2341 ccgatggtga acccgcaaga agagctggtc atttgtacct ctgcgggcat ggtcaagctc
     2401 aaattcaatg ccgatggctc ggtcagcagc acgatcagcg cacaggcgca gtgcccgctg
     2461 tgcgtggtgg gcggcgcacc accgggcttt gtccgcctgg tgattgcgcc ggtgccagcg
     2521 ccaggcctgg tgttgcaagg cattgctgcc acgcctattg cagtggcaag cgccgcaccg
     2581 ccgccctcgc gcggaccccc aagccccctc taaacaatca aattgatagc tgacagcgca
     2641 cgtccgacgt gggctagagg ctgatttgat gcatattttg gcggctttgc caaccccctt
     2701 gcattgacct gatcaggccc tgtcggcttg ccgatcaagc cacttcctga tcgctgatcg
     2761 ctattttatt aatagctgtc agcgcaag
//
LOCUS       DAOSLL010000623         2787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_31435_length_2787_cov_3.790996, whole genome shotgun sequence.
ACCESSION   DAOSLL010000623 DAOSLL010000000
VERSION     DAOSLL010000623.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2787
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31435_length_2787_cov_3.790996"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="PLB25_19970"
     CDS             complement(<1..632)
                     /locus_tag="PLB25_19970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008903155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4197 domain-containing protein"
                     /protein_id="HOE43882.1"
                     /translation="MDRRQFNSVSVSALALLATATLQQAHALTLGDISNADASRGLKT
                     ALEKGAQSAISLLGAKDGFLGNEKVRIPLPGYLEDAAKWLRTFGQGAQVDELVTSMNR
                     GAEAAVPMAKDLLVKAVETMSVTDAKSILTGGETAVTEFFATKTRPTLATKFLPAVTK
                     ATEKVGLAGKYNQVAGKASELGLVKKEDANIQQYVTGKTLDGLFFMISEE"
     gene            631..1146
                     /locus_tag="PLB25_19975"
     CDS             631..1146
                     /locus_tag="PLB25_19975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HOE43883.1"
                     /translation="MNTSSHLKRNLAIATIVAVVALFAGNYLWGASTPAPASTFVLLD
                     GTRKTTADLKGKVTLVNFWATSCVTCVAEMPKLVSTFEKYKSQGYGTIGVAMAYDQPN
                     YVVHFVQSRKIPFEVAIDNTGAVAKAWGDVRLTPTTYLVNKRGEIVKQYVGEPDFAEL
                     HRLIESLLRDT"
     gene            1419..1913
                     /locus_tag="PLB25_19980"
     CDS             1419..1913
                     /locus_tag="PLB25_19980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HOE43884.1"
                     /translation="MTYSPVNIQLIAASSPLALSGVRQLFEEYASSIGVDLCFQDFAR
                     ELAELPGDYATPRGALMVCTVDGVDAGCCAMRPLDAVDYPNACEMKRLYVRTVFRGLG
                     LGRRLAEAILDAARMAGYDCILLDTLDEMESARALYAELGFSEIPPYYYNPIAGAHYL
                     KADL"
     gene            complement(1932..2438)
                     /locus_tag="PLB25_19985"
     CDS             complement(1932..2438)
                     /locus_tag="PLB25_19985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013800250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HOE43885.1"
                     /translation="MIKVGDSLPAATLMEYSEVDGEGCSIGPNPVDVSKAVAGKTIAL
                     FALPGAYTPTCSAKHVPGFVESADAFKAAGVDEIWCVSVNDAFVMGAWARDQKTGGKV
                     RMLADGSADFAKATGLTLDLTSRGMGLRSNRYSMLVKDGKVATLNIEGPGKFEVSDAG
                     TLLAQAKA"
     gene            complement(2528..>2787)
                     /gene="rpsQ"
                     /locus_tag="PLB25_19990"
     CDS             complement(2528..>2787)
                     /gene="rpsQ"
                     /locus_tag="PLB25_19990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HOE43886.1"
                     /translation="KKSLKRTLIGKVVSDKRAKTVTVLIERRVKHELYGKIVAKSSKY
                     HAHDEKGEYKMGDIIEITESKPISKTKNWVATRLVEKAAAV"
BASE COUNT          547 a          830 c          753 g          657 t
ORIGIN      
        1 tcttcctcgg agatcatgaa gaacaggccg tcgagcgtct tgccagtgac atattgctgg
       61 atattggcgt cttctttctt gaccagtccc agttcggatg ccttgccagc cacctggttg
      121 tacttgccgg ccaatccgac tttctcggtc gccttggtta cggcgggcag gaatttggtg
      181 gccagcgtgg ggcgcgtctt ggtcgcgaag aactcggtca ccgccgtctc gccccccgtc
      241 agaatggact tggcatcggt cacgctcatg gtctccaccg ccttgacgag caaatcctta
      301 gccatgggca ccgccgcttc cgccccccgg ttcatggaag tgaccaactc atcgacctgc
      361 gcaccctgcc cgaatgtgcg cagccatttc gcagcgtctt ccaggtagcc cggcaacggg
      421 atgcgaactt tttcatttcc caggaatcca tctttggcac caagcaaact gatcgcagac
      481 tgcgcccctt tttccagggc cgtcttgagc cccctggagg catcggcatt ggaaatgtca
      541 cccaacgtga gtgcatgggc ttgttgcaat gttgccgtgg ccagcagggc cagcgcagat
      601 accgaaaccg aattaaactg acgtcgatcc atgaacacct cttctcattt gaaacgcaac
      661 ctggcaattg ccaccatcgt agcggttgtt gcgctgtttg ccggcaatta tctgtggggc
      721 gccagcacgc cagcgccggc ctccaccttc gtcctactgg acggcacgcg caaaacgacc
      781 gcggatctga aaggcaaggt gacgctggtc aatttttggg ccaccagttg tgtgacctgt
      841 gtcgccgaga tgcccaaact ggtttcgacg ttcgagaagt acaagagcca gggctacggc
      901 accattggtg tcgccatggc ctacgaccag ccgaattacg tggtccattt tgtccagtcg
      961 cgcaagattc cttttgaagt cgccatcgat aacaccggcg ctgttgccaa ggcgtggggc
     1021 gatgtgcgtc tgacccccac tacctacctc gtgaacaagc gcggagagat tgtcaagcaa
     1081 tatgtgggtg agcccgattt cgctgagttg caccgcctga ttgagtcact tttgcgggac
     1141 acctgaagct actgcagggc aacgactcca agtacttttg tatgacacgc ggaatgtcgt
     1201 ggcacggtct tgcagtttcc acgcaccgca cgtacggcgg tcttgatgct gtcgagatcg
     1261 cctgttttgc agcggcactg cgtttgctac cttgcttctg cattttttgg ccttccgtga
     1321 cacggtgggc acacgcctcc tcgcactcta cacgcgcgcg atttgtggtg tggcagcaac
     1381 cgctttgcta cgatgggcgt cctgaaatcc gatcttgaat gacttattca cccgtgaaca
     1441 ttcaacttat cgcagcgtcg tcgccactgg cgctgtccgg agtccgacaa ctctttgagg
     1501 agtacgccag ctccataggc gtggacctct gcttccagga ctttgcgcgt gaactggcag
     1561 aactgcctgg cgactatgcg actccccgcg gggcgcttat ggtatgcacg gtggatggcg
     1621 tagacgctgg ctgctgtgca atgcgaccac tcgatgcggt ggactaccca aatgcttgcg
     1681 agatgaaacg gctgtatgtc cgcacggtat tccgtggcct gggtttggga cggcgtctgg
     1741 ccgaagccat cctggacgcc gcacgcatgg caggatatga ctgcatcctg ctggatacgc
     1801 tggacgaaat ggagtcagcg cgcgcactct acgccgagct tggcttttcc gagatcccgc
     1861 cgtactacta caaccccatc gctggggcac attacctcaa ggcagatttg tagtacgcgg
     1921 gaaacttgca actaggcttt ggcctgggcc agcaaggtgc cagcgtcact gacctcgaac
     1981 ttgcccggtc cctcaatatt gagggtggca actttgccgt ccttcacaag catggaatag
     2041 cggttgctcc gcaaacccat accgcgcgaa gtcaggtcca gtgtcagacc ggtcgctttt
     2101 gcaaagtcgg cgctgccatc ggcaagcatg cgcaccttcc cgcccgtctt ttgatcccgt
     2161 gcccatgcgc ccatcacaaa agcatcattg acactaacgc accagatctc atccacgccc
     2221 gctgccttga aggcatctgc agactctacg aaaccaggta cgtgtttggc cgaacacgtg
     2281 ggggtgtaag caccaggaag cgcaaacaag gcgatggtct ttcccgcaac ggctttggat
     2341 acatctaccg gattgggtcc aatactgcac ccctcgccgt ccacctcgga atactccatc
     2401 aatgtggccg ccggaaggga atcgcctact ttaatcatct ctgctcctcg tgtttgaatc
     2461 taccaatgaa aacggcccac attgtgggcc gttcttgcaa gacttgcaga agtcgtcgtc
     2521 aatagactta aactgctgct gccttttcca ccaaacgggt cgcgacccag ttcttggttt
     2581 tggagatggg cttgctctcg gtgatttcga tgatgtcacc catcttgtac tcgccctttt
     2641 catcatgggc gtggtacttg ctggatttgg caacgatctt gccgtagagc tcgtgtttta
     2701 cgcggcgctc aatcagcaca gtcactgtct tggcacgctt gtcgctgacc accttgccaa
     2761 tcaaggtgcg cttgagggat tttttag
//
LOCUS       DAOSLL010000624         2786 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_31454_length_2786_cov_3.235811, whole genome shotgun sequence.
ACCESSION   DAOSLL010000624 DAOSLL010000000
VERSION     DAOSLL010000624.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31454_length_2786_cov_3.235811"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="PLB25_19995"
     CDS             complement(<1..699)
                     /locus_tag="PLB25_19995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HOE43887.1"
                     /translation="MTDVSLQMQLVNGQLPVHAYFDEALYQREMQTLFQRGPRYIGHT
                     LSVPNVGDYAALPQEGGGRALVHTVNGIELVSNVCRHRQAIMLKGRGSLNQAGTGGNI
                     VCPIHRWTYSSSGSQPAGTLLGAPHFPHGPCLNLNNYPLQEWNGLLFEAPSPNSPEVG
                     GRDVATDLAGLTDHAALDFSGFVLDRVELHECNYNWKTFIEVYLEDYHVGPFHPGLGH
                     FVTCDDLRWEFADHY"
     gene            1121..1651
                     /locus_tag="PLB25_20000"
     CDS             1121..1651
                     /locus_tag="PLB25_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43888.1"
                     /translation="MPFTPFHLGAAMLVKPAAGSRFSVISFGLAQIVIDIEPGIGMLR
                     GTETLHGASHTVIGAILIALAVAWVSPWLSSRLIRRWNLAVRHCQLDWLTEPASASKG
                     ASTAGAFFGTFSHIGLDCLMHHDIHPLMPFSSANPLIGLVSHDGVYQLCLLMGVLGTA
                     AWLLLKWLRRGVSPLA"
     gene            1724..2044
                     /locus_tag="PLB25_20005"
     CDS             1724..2044
                     /locus_tag="PLB25_20005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43889.1"
                     /translation="MPGISIFPDWGLGISLVMTVTTLATAQWSVSVLRQRRWHGLALA
                     AFWIWLSVHGIHWAHWSIVRPQIMIREGQWLAPPCLYALCGVIWYRLVPMLADLHGRW
                     QKLP"
     gene            2133..2369
                     /locus_tag="PLB25_20010"
     CDS             2133..2369
                     /locus_tag="PLB25_20010"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010103375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HOE43890.1"
                     /translation="MATASKHGPAAPANYEAALQELELLVARLESGELPLEQLLAQYQ
                     RGAELLKFCRDRLEAVEGQVKVLDQGVLKPWTPD"
     gene            2500..>2786
                     /locus_tag="PLB25_20015"
     CDS             2500..>2786
                     /locus_tag="PLB25_20015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43891.1"
                     /translation="MQSAFDLNLWMARQLDAVERALNAWVSVDASADLDHHAPAELMA
                     AMRYAVLDGGKRLRPLLVLAAFEAVRADPKPPPPGQAELDPATLHAAPQDLS"
BASE COUNT          558 a          785 c          832 g          611 t
ORIGIN      
        1 atagtggtcg gcaaattccc agcgcaggtc gtcgcaagtg acaaagtgcc ccagaccggg
       61 atgaaatggc ccgacatggt agtcctccag atagacctcg atgaaggtct tccagttgta
      121 gttgcattcg tgcagttcta cccggtcgag cacaaagccg gaaaagtcga gggccgcgtg
      181 atcagtcagg ccagccagat cggtcgccac gtcgcgcccg ccgacctccg ggctattggg
      241 tgatggtgct tcaaacagca ggccattcca ctcctgcaac ggatagttgt tgaggttcag
      301 gcaagggcca tggggaaaat gcggcgcacc cagcaaggtt ccggcaggct gggaacccga
      361 gctgctgtag gtccagcggt ggatcgggca gacgatgttg cccccggtac cggcctggtt
      421 gagtgaaccg cggcctttga gcatgatggc ctggcgatgg cggcagacgt tggaaaccag
      481 ttcaatgccg ttgaccgtgt gcaccagggc gcgaccgccg ccttcctggg gcagggcagc
      541 atagtcaccc acattgggca cactcaaggt gtgaccgatg taacgaggtc ccctttgaaa
      601 cagggtctgc atttcccgct ggtacagcgc ctcgtcaaag tatgcatgaa ctggcagttg
      661 gccgttgacc aactgcattt gaagacttac atcagtcata gatgacctga ccccagtgca
      721 ggtaacgtgg gatagacaaa acccatgcct gcttcagaac caggcgacat gctgcttgcc
      781 ctggtcgcac cttgggatag ccaacggctt caagccttct cttgcattga tctgggaacg
      841 gcatgacttc ttgatttcaa acaattgctt tagccctgat ttttccacat gtccaacaaa
      901 aagcggcgca actcgggtca tcgcgcatct agcctgccca agcattgggc aaaaaactgt
      961 ctgcaaaaat tcctggccgc aaaagctgaa ggggggattt tgccctacgg tcaagaacca
     1021 tcgccgaact gccgtcgatg ccagcggtga ggggtcagcc gttcccaagg ttttgctacc
     1081 atggcccgac catacctggt gcaacgagga tttagtcacg atgcccttca ctcctttcca
     1141 tctgggtgca gcgatgctgg tcaaacctgc tgccggttcc cgtttcagcg tgatttcctt
     1201 cggtctggcg caaattgtga tcgacattga acctggcatt ggcatgctgc gcggcaccga
     1261 gaccttgcac ggcgcttcgc acaccgtcat cggggccatt ctgatcgcgc tggcggtcgc
     1321 gtgggtgtcg ccctggcttt cttccaggct gattcgcagg tggaatctgg cggtccggca
     1381 ttgccagctt gactggctga ctgagccagc atcggcaagc aagggtgcca gcacggcagg
     1441 cgcttttttc ggcaccttca gccacattgg tctggactgt ttgatgcatc acgacatcca
     1501 ccctttgatg ccattttcca gtgccaatcc actcattggc ctggtttctc atgacggtgt
     1561 ttatcagttg tgcctgctga tgggggtgct gggcacggcc gcatggctat tgctgaaatg
     1621 gttgcggcgc ggtgtgtcgc cgcttgcatg aaacaggtca acagcttgcc gctgtggctc
     1681 aaaatgggca gctttgcggt cggactgggc ctgttgccat accgtgcctg gtatctcgat
     1741 ttttccggat tggggcctgg gcatcagtct ggtgatgacc gttaccaccc tggccacagc
     1801 acagtggtcg gtttcggtgc ttcggcagcg ccgctggcac gggctagcgc tggcggcctt
     1861 ctggatctgg ctgtcggttc atggcatcca ttgggcccac tggtccatcg tcagaccgca
     1921 gatcatgatt cgtgaggggc agtggctggc accgccgtgc ctttatgcct tgtgcggggt
     1981 gatctggtac cggctggtac cgatgctggc tgatttgcat ggcagatggc aaaaactgcc
     2041 ttgacaacct ttgccaagca tagccagagc gggcagggcg gctgtggcaa agcctaaaat
     2101 cagtcttttt gcaccagaga gtattgcgtt ttatggcaac agcctccaaa cacggtccag
     2161 cggcacctgc caactatgag gccgccctgc aggaactcga actactggtg gccaggctag
     2221 agtcgggcga gctgccgttg gaacaactgc tggcgcagta ccagcgcggt gccgaactgc
     2281 tgaaattctg ccgtgaccgc ctggaagcgg tggaaggcca ggtcaaggtg ctcgatcaag
     2341 gcgtgcttaa accatggaca cccgactaga tcatcccgga ttcctcagtt ggatgcctgg
     2401 cgctgtgacc taatgggtga accccttcga agctaaaaaa atgaatgctc ataaggactt
     2461 acagatgaaa acgagcggtc aatcgaggaa tcttggaaca tgcaaagcgc atttgatttg
     2521 aacctctgga tggcgcgcca gcttgatgcc gttgagcgtg ccctgaacgc ctgggtcagc
     2581 gtggatgcat cggctgatct ggaccaccat gcacccgctg agctgatggc cgcgatgcgc
     2641 tatgccgtgc ttgacggtgg caagcgcctg cgtccgctgc tggtgctggc ggcgtttgag
     2701 gcagtgcgtg ccgacccgaa gccgccaccg cctggtcaag cagaacttga tccagccaca
     2761 ctgcacgcag cgccgcaaga cttgtc
//
LOCUS       DAOSLL010000625         2785 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_31467_length_2785_cov_4.089011, whole genome shotgun sequence.
ACCESSION   DAOSLL010000625 DAOSLL010000000
VERSION     DAOSLL010000625.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31467_length_2785_cov_4.089011"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..742)
                     /locus_tag="PLB25_20020"
     CDS             complement(<1..742)
                     /locus_tag="PLB25_20020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HOE43892.1"
                     /translation="MQTTFPRLLLNHAVGRPADIAMREKEYGIWQAITWQEMAAMVEH
                     LACGLHQAGLRQHDHMVVIGANRPRLYATMLAVQALGAVPIPLYQDAAAMECVFPLNN
                     ADVCFAFAEDQEQVDKLMEIREQCPQLKHVYFDDPRGLRNYDEPGLAALDELVAAGRA
                     FAAIHIDFFKNEVDKVRPDDVAAMFFTSGTTGNPKGVVHTHDSLLNRARAGADFDRLT
                     HAEDVLAYLPPAWIGQNIFSYAQWLSCGY"
     gene            921..1634
                     /locus_tag="PLB25_20025"
     CDS             921..1634
                     /locus_tag="PLB25_20025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007875060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HOE43893.1"
                     /translation="MSPEPSLFHRRRRLTTAELDVIPWLQRLLPAERERAAEDLQIAD
                     AVPGDYICRTGRPVTYWFGAIDGLLKMSADNAEGQTMTFTGIPPGGWFGEGTALKREA
                     YRYNIQPLRKSLVAGLHVDTFHWLLDHSIGFNRFVMNQLNERLAQFIAAREIDRMSNP
                     NIRVARSLAALFNPVLYPGLGTVLRITQQELAYLVGLSRQRVNEALTLLAAHNAIQVE
                     YGGLRVTDLQALRTSLFLA"
     gene            complement(1771..2781)
                     /locus_tag="PLB25_20030"
     CDS             complement(1771..2781)
                     /locus_tag="PLB25_20030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HOE43894.1"
                     /translation="MENPNMLQKSTNIQVATAGEALIDLIAGVDGCLQPCLGGAVYNL
                     TRALARQGIETLYLNPLSKDRFGRQLAAALTQDGAQLAVPDAVQQVTSLAVVNVDASG
                     HPDYAFYREGVADRATNAAELTHACSQLESLSLVCTGALALSPDDGETYLPWLAAQRQ
                     AGRTVVIDANLRPSVMPNLDLYRRHVLTALQYADVIKASDEDLDCLRLPGSSAAAQAQ
                     HLLQYSRASVLALTRGSEGAALLTRHGQLFQARETMPLAVVDTVGAGDCFLAGLVASM
                     LQHKLATDWGSATVKEEVARVLLSSAIASASLCVMQRGCVPPTRAEVLQRMGSVEMAF
                     VV"
BASE COUNT          542 a          880 c          822 g          541 t
ORIGIN      
        1 cgtaaccaca ggacagccac tgcgcgtagc tgaagatgtt ctggccaatc caggccggtg
       61 gcaggtaagc cagcacgtct tcggcatggg taaggcggtc aaagtcagcg ccggcacggg
      121 cccggttcaa cagtgaatca tgggtatgca ccacgccctt ggggttgccg gtggtgccgg
      181 aggtaaagaa catcgccgcg acatcatcgg gccgcacctt gtccacctcg tttttgaaga
      241 aatcgatgtg aatggcagca aatgcccggc cagccgctac cagttcatcc agcgccgcca
      301 gccccggctc gtcgtaattg cgcaggccgc gcgggtcatc aaagtaaaca tgcttgagtt
      361 gcgggcattg ctcgcggatt tccatcagct tgtccacttg ctcctggtct tcggcaaagg
      421 caaagcagac atcggcattg ttgagcggaa acacacattc cattgcggcc gcgtcctggt
      481 acagcggaat cggcacggca ccgagcgcct gcaccgccag catggtggcg tacagacgcg
      541 gccggttggc accgatcacc accatgtgat catgctggcg caagccagcc tggtgcagcc
      601 cgcaggccag atgctcaacc atggccgcca tctcctgcca ggtgatggct tgccagatgc
      661 catattcttt ttcgcgcatg gcaatgtcag ccgggcggcc gacggcatgg ttcaacagca
      721 gtctgggaaa agtcgtttgc atcggggcac cttgtctgtc cgcatggtca ccagccaaag
      781 ccgtttggca attgctgatc tggtcgcaat gataggactc gatttgacgc taagttgtcg
      841 tttgggcgac aattcagagc tttctcggta ggtgttttcc ctgtattttt tcaagcccaa
      901 ggcaaccacg gaagtccatc atgagccctg aacccagtct ttttcatcgc cgtcgccgct
      961 tgaccacggc tgagctggac gtgattccgt ggttgcagcg gctgctgccg gctgagcgcg
     1021 agcgtgccgc cgaagacctg cagattgccg acgcagtacc gggcgactac atctgccgca
     1081 ccggccgacc ggtgacctac tggtttggtg cgattgacgg cctgctgaaa atgagcgccg
     1141 acaacgccga gggccagacc atgacgttta ccggtatacc gccgggcggc tggtttggcg
     1201 agggcaccgc cctgaaacgc gaagcctacc gctacaacat ccagccgctg cgcaaaagcc
     1261 tggtggccgg tctgcatgtg gacacctttc actggctgct cgaccattca attggtttta
     1321 accgttttgt catgaaccag cttaacgaac ggctggccca gttcatcgcg gcgcgcgaga
     1381 ttgaccgcat gagcaacccg aacatccgcg tggcacgctc gctggcagca ctgttcaacc
     1441 cggtgctcta ccccggcctt ggcacggtgt tgcgcatcac ccagcaggaa ctggcgtatc
     1501 tggtgggtct gtcacgccag cgggtcaatg aggcgctgac tttgctggcc gcgcacaatg
     1561 ccattcaggt ggagtatggc ggcctgcggg tgaccgattt gcaagccttg cgcaccagtt
     1621 tatttttagc atgaaattgg cctctggccc ttgtcaatag tgcgctgaca gctactgttt
     1681 tgaaagcatc agagaggctg gcggcgcgcc acccgccgca gcgccgagcg ctcctaaaaa
     1741 gttgcagcca cctgtaccgc cgggaacccg ctagacaaca aaagccatct cgaccgaacc
     1801 catccgctgc agcacctcgg cgcgcgtagg tggcacgcag ccgcgctgca tgacgcacag
     1861 gctggcactg gcaatggcac tgctcagcag caccctggcg acctcttctt tcaccgttgc
     1921 cgagccccag tctgtggcca gcttgtgctg cagcatcgat gccaccagcc cggccagaaa
     1981 gcagtcgccc gcgcccaccg tgtccaccac cgccagcggc attgtttcgc gcgcctgaaa
     2041 caactgcccg tggcgggtca atagcgccgc accctcactg ccacgggtca gtgccaacac
     2101 gctggcgcgg ctgtactgca gcagatgctg tgcctgtgcc gctgcgctgc tgcccggcaa
     2161 gcgcaggcaa tcaaggtctt cgtcactggc cttgatgaca tcggcatatt gcagtgccgt
     2221 caatacatgg cggcgataca ggtccagatt gggcatcacc gagggccgca aattggcatc
     2281 gatcaccacc gtgcgaccag cctggcgttg cgccgccagc cacggcaggt aggtttcgcc
     2341 gtcgtcaggc gacagcgcca gcgcaccggt gcataccagc gacaggctct ccaactggct
     2401 gcaggcgtgc gtcagttcgg cggcgttggt ggcccggtct gccaccccct cgcggtaaaa
     2461 ggcgtaatcc gggtgaccgc tggcatcgac attgaccacc gccagcgaag tgacctgctg
     2521 caccgcgtct ggcaccgcca attgcgcacc gtcttgcgtc agggcagcgg ccaactggcg
     2581 gccaaaacgg tcttttgaca ggggattgag gtagagcgtc tcaataccct ggcgcgccag
     2641 cgcccgcgtc aggttataga cagcgccgcc caggcagggc tgcaagcagc catcaacacc
     2701 cgcaatcagg tcaatcaggg cctcgccagc ggtggcaact tgaatgttgg tacttttttg
     2761 cagcatatta gggttttcca taact
//
LOCUS       DAOSLL010000626         2770 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_31673_length_2770_cov_4.676611, whole genome shotgun sequence.
ACCESSION   DAOSLL010000626 DAOSLL010000000
VERSION     DAOSLL010000626.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31673_length_2770_cov_4.676611"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="PLB25_20035"
     CDS             <1..271
                     /locus_tag="PLB25_20035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43895.1"
                     /translation="LLIGSVGALWVLQVDAVEHSWIARVIKLLFLPALLCLVCLPFFP
                     VSSNHPGMDATLICLSTIIVILRNSGWLSSTRSISVLAVIGDFSW"
     gene            443..1621
                     /locus_tag="PLB25_20040"
     CDS             443..1621
                     /locus_tag="PLB25_20040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012212527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HOE43896.1"
                     /translation="MAQRTQLDDADNPLHEAYECLLANGLDGAGEALRILVNEASRIE
                     RAQHLQATPYERSAQRVDYANGYKSKTVLTRLGEVTFEVPQVRSGGFYPSALERGSRS
                     EQAMNLALAEMYVQGVSTRKVITVLQKLVGPEISISSTQISRCTALLDEGLQAWRTRP
                     LDETPYVILDARYERVRHANQVVDCAVLVAIGITGTGHRRVLGVSVALSEAEVHWRAF
                     LDSLIQRGLRGVKYIASDDHAGLKAARKAMFPGVTWQRCQFHLQHNAQGYVSKLDRRT
                     PVARQIRAIFNAGDAHEAQRLLNLALKDWQVSHPQLAAWAETNLPEGFAVFNLPDSHR
                     VRMRTTNGLERLNKELKRRTRVATLFPNPESCQRLVSALLAEQDEEWMTAKIYLNMKP
                     "
     gene            complement(1657..2636)
                     /locus_tag="PLB25_20045"
     CDS             complement(join(1657..2168,2168..2636))
                     /locus_tag="PLB25_20045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558169.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HOE43897.1"
                     /translation="MAKRGLSEAAKKRVKAGRLLLEGKGCAEVALAVGVARQTVYTWK
                     KLLDEGGIDALREVPERGRPAQLDEQQLASIRAAVLQNPSEHGFGTELWTLKRVGVII
                     ERLHGIRFSQTQTWRILGALGFSPQKPEKRAIERNEDAVRAWKRSTWPAIKKKPLREG
                     RLIVFVDESGISERPTRVRTWAPKGQTPIIQFHFNWNHVSVIAGLTRTNCLFRLHEGS
                     IKKEEIVEFLKALKAHLKQPLLVIWDGLKAHRSRLVREHLDGLNGHIQIAFLPPYAPD
                     LNPVEYLWAWLKRHALANYCPNNLSELHTTARNKLKSAQKRIKQLLFCKF"
BASE COUNT          565 a          773 c          787 g          645 t
ORIGIN      
        1 attgcttatt ggctcggttg gcgcactgtg ggtactgcag gtcgatgcag ttgagcatag
       61 ctggatagct cgtgtgatca agttgctctt tttaccggct ttgctgtgtt tggtgtgctt
      121 gccgttcttc cctgttagca gcaatcaccc cggcatggac gctacgctca tttgcctttc
      181 aaccattatt gttattttgc gcaatagcgg ttggctcagt tccacgcgga gcatatcggt
      241 attggctgtc atcggggact tttcttggta gtgcaacatt cgttctgtaa tttcccgaac
      301 caacgaagca agactcgctg agaattgttt ctgaattcgg ttttggccat ttttagattc
      361 aggcccctgc caggccgccg gaggcacccc ccccggcgat tgcttggcca ggcctgagcg
      421 acacagaaat aatgagttca ccatggccca aagaacccaa cttgacgatg ccgacaaccc
      481 gctgcacgaa gcctacgaat gcctgcttgc caatggcctt gatggcgctg gcgaagcgct
      541 tcgaatcttg gtcaacgaag cctcgcgcat tgaacgcgcc cagcacctgc aggccacacc
      601 ttacgagcgt tcggcccagc gcgtggacta cgctaacggc tacaaatcca aaacagtact
      661 aacccgcttg ggcgaggtca cctttgaggt cccgcaagtg cgctcgggag gtttttaccc
      721 cagcgcactg gagcgcggca gccgctccga acaggccatg aatctggctt tggcggagat
      781 gtatgtgcag ggtgtctcca cccgtaaagt catcaccgta ctgcaaaagc tggtggggcc
      841 ggaaatcagc atctcatcga cgcaaatcag ccgctgtacc gcactgcttg atgaaggact
      901 gcaagcctgg cgaacccgac ccctggatga gacgccctat gtgattttgg atgcccgtta
      961 tgagcgggtt cgtcacgcca accaggtggt ggactgtgcg gtgctggtgg ccattggcat
     1021 cactggcacc ggacaccgcc gggtgctggg cgtgtcggtg gcactctcag aagccgaggt
     1081 gcactggaga gcctttctgg acagcttgat tcagcgtggc ttgcgtgggg tcaaatacat
     1141 tgccagcgat gaccacgcgg gtctcaaagc agcccgcaaa gcgatgtttc ccggtgtgac
     1201 gtggcagagg tgccagtttc atctgcaaca caacgcgcag ggctatgtct ccaagctcga
     1261 tcggcgcacg ccggtggcca ggcagatacg cgccatcttc aatgctggcg atgcccatga
     1321 ggcccagcgt ctgctcaatc tggcactcaa ggactggcag gtgagtcacc ctcaactggc
     1381 tgcgtgggct gaaaccaatc tacccgaggg gtttgcggtg ttcaatttgc cagatagcca
     1441 tcgggtgcgc atgcgcacca ccaatggtct ggagcgcttg aacaaggagc tcaagcggcg
     1501 aacccgtgtg gccacgctgt ttcccaaccc ggaaagttgt caacgattag tcagcgcact
     1561 gctggccgaa caagatgagg agtggatgac agcaaaaatc tacctgaaca tgaagccttg
     1621 acccgacgct attgcgcgac aaccccagaa ccaaaatcag aatttacaga aaagaagttg
     1681 ctttatccgt ttttgagcac tcttgagttt gttgcgtgcg gtggtgtgca actcgctcag
     1741 gttgttgggg cagtagttgg ccaaggcgtg tcgtttgagc caggcccaca agtattcgac
     1801 cggattcaag tccggcgcgt agggcggcag aaaggcaatc tggatgtggc cgttcaatcc
     1861 gtcaaggtgc tcgcgcacca agcgactgcg atgcgccttc agcccatccc agatcaccaa
     1921 cagcggttgc ttcaaatgcg ctttgagcgc cttgaggaat tcgacaatct cttccttctt
     1981 gatactgcct tcgtggagtc ggaacaggca gttggtacgg gtgagtcccg cgatgacgga
     2041 gacgtgattc cagttgaagt gaaactgaat gatgggtgtc tgacccttgg gtgcccaggt
     2101 gcgcacccgg gtcggacgtt cgctgatacc ggactcgtcg acaaagacga tcagtctgcc
     2161 ttctcgcagg gctttttttt aatggctggc caggtgctgc gcttccaggc gcgaacagca
     2221 tcttcattgc gttcaatggc gcgtttctcc ggcttttgcg ggctaaagcc caaggcaccc
     2281 aggatgcgcc aggtttgcgt ctggctgaaa cgtatgccgt gcaggcgctc aataatgaca
     2341 cccacccgct tgagggtcca caactctgtg ccaaatccgt gttcgctggg gttttgcagc
     2401 acggctgcac gtatcgatgc caattgctgc tcgtcgagct gggctgggcg accacgctcg
     2461 ggtacctcgc gcaaggcgtc gatgccgcct tcatcgagta gcttcttcca ggtgtatacg
     2521 gtctggcgcg cgacgccaac agccagtgca acttcggcgc accctttgcc ttcgagcagc
     2581 agtcgacctg ccttgactcg tttctttgcc gcttcgctta gccccctttt tgccatgacc
     2641 gcattctcca atattgctac tggagataca acgcacgagc gcctcatcgg ttgtcatgaa
     2701 ttatgaaatt atcaataaat tcagttttca tggtcgactc ctgtacgggg attgggtttg
     2761 ctgactgatg
//
LOCUS       DAOSLL010000627         2762 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_31803_length_2762_cov_3.471371, whole genome shotgun sequence.
ACCESSION   DAOSLL010000627 DAOSLL010000000
VERSION     DAOSLL010000627.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31803_length_2762_cov_3.471371"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="PLB25_20050"
     CDS             <1..1148
                     /locus_tag="PLB25_20050"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010104564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HOE43898.1"
                     /translation="LFFTQSPSPAMVRVAVTTLADASYVYTTTKSEAEGRSIQKASFV
                     LPEDEGLRAGFGMAVAAVTGIEFAKEWGNRPANHATPAKLAQAARSLAKLPRIKCKVL
                     GPKDVAKLGMGAFEAVAQGSDEPLRFIELAYNGAGKSSPPVVLVGKGISFDSGGISIK
                     PAAEMDEMKFDMCGAASVLGVFRALADLQPDINVVGLIPACENLPSGRAVKPGDVVTS
                     MSGQTIEVLNTDAEGRLVLCDALTFAEQFKPRVVLDIATLTGACVIALGGVRSGFFTT
                     DQSLSAALLSAGDSSNDLCWPMPLDDEYAEGLKSNFADVANVAGRTGGAITAAKFLQR
                     FTGKYVWAHLDIAGTAWKGGTTKGATGRPVGLLLDYLMAMNSPAKKA"
     gene            1145..1576
                     /locus_tag="PLB25_20055"
     CDS             1145..1576
                     /locus_tag="PLB25_20055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006298370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HOE43899.1"
                     /translation="MTTVAFHLQVPDKFTYACRLLRKAVAKGARLVVTADAPTLARLN
                     QDLWTFSGADFVPHCHDSAPDRVLRRSPVVLAESPKASDQTMTLINLGHQVPTGFDGF
                     GQVIEVIPMEDEDLRLARERWKYYTKAGYKPVMHDIGSKTV"
     gene            1573..1884
                     /locus_tag="PLB25_20060"
     CDS             1573..1884
                     /locus_tag="PLB25_20060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43900.1"
                     /translation="MNSPTKNLPRFVPTLTEVVDPASLTGLAVAAKPDVEALISLVQR
                     QIQPIFERRLQDEFDRLMRASISKQWADVSSRLQDEMDMFVRQAVIDAVGKSEQAKTP
                     K"
     gene            2007..>2762
                     /locus_tag="PLB25_20065"
     CDS             2007..>2762
                     /locus_tag="PLB25_20065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HOE43901.1"
                     /translation="MQMKLKITVAAAIAAAAGVAFAQDMVVKIGHVAPLSGSQAHYGK
                     DNENGVRMAIEDLNAQKIVIGGKTIKFEIAAEDDAADPKQGTAVAQKLCDAKVAGVVG
                     HLNSGTTIPASKIYNDCGIPHITGAATNPNLTKPGYKTTYRIIANDLALGGGLAVYAA
                     DVMKLKKFAVIDDRTGYGQPLAEVFMKDAKLKGIEIVDQQFTNDKASDFMAILTQIKS
                     KKPDAIFFGGMDPQAGAMLRQMEQLGLTNVKYFG"
BASE COUNT          658 a          706 c          783 g          615 t
ORIGIN      
        1 cgctgttttt cactcaaagt ccaagccccg caatggtacg ggtagccgtg acaaccctgg
       61 ccgatgccag ctatgtttac accacaacca aatccgaggc agaagggcgc tccattcaga
      121 aagccagctt tgtcctgcca gaagatgaag gcttgcgtgc gggtttcggc atggcagtag
      181 cggcagtaac cggtatcgag tttgccaagg aatggggcaa ccggccggcc aaccacgcaa
      241 cgccagccaa attggctcag gcggcgcgct cgctggccaa gttgccacga atcaagtgca
      301 aggtgctcgg cccgaaagat gtggccaaac tcggcatggg tgcgtttgag gcagtggccc
      361 aggggtctga cgaaccgctt cgcttcattg aactggccta taacggtgcg ggcaagtcat
      421 cgcctccagt ggtgctggtc ggcaagggca tcagctttga cagtggcggc atctccatca
      481 agccagcggc tgaaatggat gagatgaaat ttgacatgtg tggtgctgcc agcgtgctgg
      541 gggtgttccg ggcactggct gatttgcaac ctgacatcaa tgtggttggc ttgattccgg
      601 cctgcgaaaa cctgcccagt ggccgtgcag tcaaaccggg tgatgtcgtg acgagcatga
      661 gcggacaaac cattgaagta ctcaataccg acgcggaagg cagactggta ttgtgtgatg
      721 cgctcacatt tgcagagcaa ttcaaaccac gcgtggtgct cgacattgcc actttgactg
      781 gtgcctgtgt gatcgccttg ggtggtgtgc gcagtggttt tttcaccacc gatcagtcct
      841 tgtctgccgc attgctgtct gccggagact catccaatga cctgtgctgg ccaatgcctt
      901 tggacgatga atacgcagaa ggtctgaagt cgaactttgc tgacgttgcc aacgttgcag
      961 gtcgaaccgg tggtgccatc accgcagcca agttcttgca acggtttacc ggcaaatacg
     1021 tttgggctca cctggacatt gctggcactg cctggaaagg tggcaccacc aagggtgcca
     1081 ccggtcgtcc ggtagggctg ttgctggatt atttaatggc aatgaacagc ccggccaaaa
     1141 aagcgtgacc acagtagcat ttcatttgca ggtgcccgac aaattcacct acgcttgccg
     1201 gttgctgcgc aaggctgtcg ccaagggcgc gaggttggtt gtcaccgccg atgcgccaac
     1261 gctggccagg ctgaatcagg acttatggac tttttcgggc gctgattttg tgccacattg
     1321 ccacgatagt gccccagacc gtgttttacg tcgatctcca gtcgtgctgg cagaaagccc
     1381 caaagcatct gaccagacca tgaccctgat caatttgggt catcaggtac cgactggttt
     1441 tgatggtttt gggcaggtga ttgaggtgat cccaatggaa gacgaagatc tgcggctggc
     1501 acgtgagcgt tggaaatact acacgaaagc aggttacaaa ccggtcatgc acgacatcgg
     1561 ttccaaaaca gtatgaattc accgaccaaa aacctgccga ggtttgtccc gaccctgacc
     1621 gaagttgtcg atccggccag tttgactggt ttggcagttg cggccaaacc ggatgtcgag
     1681 gccctgattt cattggtgca gcgccaaata cagccaatct tcgagaggcg gctacaggat
     1741 gagttcgacc gcctgatgcg tgccagtatt tcgaaacagt gggcagatgt cagcagcaga
     1801 ctgcaagatg aaatggacat gttcgtcagg caggcagtca ttgatgctgt tggcaagtcc
     1861 gaacaggcca aaacgccaaa atagctgatt atcccaatca gtcaaatcga ttcttggtga
     1921 ctacccttag aggatggcgc aaaaattgct gtatcgtacg cggtgctgaa ttgtccgatt
     1981 cacatttttt taacttggag ttgtctatgc aaatgaaatt gaaaatcact gtggctgcgg
     2041 ccatcgctgc tgcggctggt gtagcgttcg cgcaagatat ggtcgtcaag attggtcacg
     2101 tggcaccgct gtcgggtagt caggcccact atggcaaaga taacgaaaac ggcgtgcgca
     2161 tggccatcga agacctgaac gcgcaaaaaa ttgtgatcgg cggcaagacg atcaagtttg
     2221 aaattgctgc agaagatgat gcggctgacc caaaacaggg cacagcggtt gcacaaaagc
     2281 tgtgcgatgc caaagtggca ggcgttgtgg gtcacctgaa ttcgggtacc accatcccgg
     2341 cctccaagat ttataacgat tgcggtattc ctcacattac cggggcagct accaacccca
     2401 atttgaccaa gccgggctac aagaccacct accgcatcat tgcaaacgat cttgctttgg
     2461 gtggtggctt ggccgtgtat gcggctgatg tcatgaaact caaaaagttc gcggtgattg
     2521 atgatcgaac cggatatgga caaccgctcg cggaagtgtt catgaaagac gcgaaactga
     2581 aaggcattga aattgttgac cagcagttca ccaatgacaa ggcctccgac tttatggcca
     2641 tcctgaccca aatcaaatcc aagaagccag atgccatatt ctttggtggc atggacccgc
     2701 aagctggtgc catgctgcgt cagatggagc aactgggtct caccaacgtc aagtacttcg
     2761 gc
//
LOCUS       DAOSLL010000628         2756 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_31879_length_2756_cov_4.390966, whole genome shotgun sequence.
ACCESSION   DAOSLL010000628 DAOSLL010000000
VERSION     DAOSLL010000628.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2756)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2756
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31879_length_2756_cov_4.390966"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="PLB25_20070"
     CDS             <1..234
                     /locus_tag="PLB25_20070"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HOE43902.1"
                     /translation="RSLKKAVALAGIHKPVSVHTLRHSFATHLLQASTGTATQPGTGH
                     TSPTCYPVIQFDIILIAANAHCTRATGIKAFRP"
     gene            complement(269..1276)
                     /locus_tag="PLB25_20075"
     CDS             complement(269..1276)
                     /locus_tag="PLB25_20075"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HOE43903.1"
                     /translation="MKPSQLLTCVALLACTQSALAEEVVVYSARNEQLIKPLFDAYTQ
                     ETGVVVKFITGGEGPLMERLKAEGQNTPADLLLTVDAGNLWQASAQELLRPVSSKTLQ
                     DNVPAHLRDPGNTWFGLSVRARTLVYNTTRLKAADLSSYEDLADPKWKGRLCLRTAKK
                     VYNQSLVAMMITEHGEAKTEEIVKGWVSNLTTTPFPDDTKAMEAVANRQCDVTVVNTY
                     YFGRLMEKSPKLPLAIFWPNQNLTGKNAGVHVNVSGAGVTRHAKNPAGAQKLIEWLSS
                     ERAQNLYADSNLEYPVNPRVKPDPQVAAWGSFKPNQINVKDAGSLQTQAVKLMDRAGY
                     K"
     gene            complement(1673..>2756)
                     /gene="nifA"
                     /locus_tag="PLB25_20080"
     CDS             complement(1673..>2756)
                     /gene="nifA"
                     /locus_tag="PLB25_20080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015004755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nif-specific transcriptional activator NifA"
                     /protein_id="HOE43904.1"
                     /translation="VFDQVHKVAPARTTVLLRGESGTGKEVIARAVHNLSPRKHQAFV
                     RVNCAALTESLLESELFGHEKGAFTGAQGQRKGRFEMSHGGTLFLDEIGDISAAFQAK
                     LLRVLQEREFERVGGSTVVKVDVRLILATNRNLERMVQAGEFRADLYYRINVVSIQLP
                     PLRERREDIPVMAQHFLDKFNAENARNTQLSPAAMRVLTSCYWPGNVRELENCIERTA
                     TMAHGDVILDIDFPCQGNHCLTQVLHHMDRVDAVTPQRLADIPVLEVPLNPLPAGNFP
                     PPPPVAATASATAAGGEAPGEAAPADLDAPLPDADGKPDGERERLIWAMERCGWVQAK
                     AARLLKISPRQMGYALQKNEIEVRKF"
BASE COUNT          524 a          841 c          802 g          589 t
ORIGIN      
        1 cgttcactca aaaaggctgt tgcactggct gggattcaca agccggtgtc ggttcacacg
       61 ttgcgccatt catttgccac gcacttgctg caggcaagca ccggcacggc cacgcagcct
      121 ggcactggcc acaccagccc aacctgttat ccagtgatcc agtttgatat aattttgata
      181 gctgccaacg cacactgcac aagggctaca ggcataaaag cctttagacc ttgaaaaagc
      241 caaaaagacc aacggccccg gcccaccctt atttgtagcc agcccggtcc atcagcttga
      301 cggcttgcgt ctgcaagctg ccagcatctt tcacgttgat ctggttgggt ttgaagctgc
      361 cccaggcggc cacctgcggg tcgggcttga cgcgcgggtt gaccgggtat tccaggttgc
      421 tgtcggcgta aaggttctgt gcacgttctg aagacagcca ttcgatcagc ttttgcgccc
      481 ccgctgggtt cttggcgtgg cgcgtgacac cggcgcccga cacattcacg tgcacaccgg
      541 catttttgcc agtcaggttc tggttcggcc agaaaatggc caatggcagt ttcgggcttt
      601 tttccatcag acggccaaag taataggtgt tgaccaccgt cacatcgcac tggcggttgg
      661 ccaccgcttc catcgccttg gtgtcatctg gaaacggcgt ggtggtcagg ttactgaccc
      721 agcctttgac gatttcttcg gtcttggctt caccgtgctc ggtgatcatc atcgccacca
      781 ggctctggtt gtaaaccttt ttggcagtgc gcaggcacaa gcggcccttc catttggggt
      841 cggccaggtc ttcatagctg ctcaggtcag cggccttgag ccgggtggtg ttatagacca
      901 gcgtgcgtgc gcgcaccgac aggccaaacc aggtgttgcc agggtcgcgc aggtgggctg
      961 gcacgttgtc ctgcagggtt ttggaactga ccgggcgcaa cagctcttgc gccgaggcct
     1021 gccacaggtt gccggcatcg actgtcagca gcaggtcggc cggcgtgttc tgcccctcgg
     1081 ccttgagtcg ctccatcagc ggaccttcgc caccggtaat gaacttgacc acgacaccgg
     1141 tttcctgcgt gtaggcatca aacagtggct tgatcaattg ctcattgcgg gcggagtaaa
     1201 ccaccacttc ttcagcaagc gcgctctggg tgcaggccag caacgcaaca caggtcaaca
     1261 attggcttgg cttcatctga tcaggtcttt caaagtaagg gtatcaatac aaccagatca
     1321 aaacgacgta aagctgagct tggccgcctg cttcgtttga cctggagcaa attgtaacaa
     1381 atgggaataa ttcgcatata gacgacccag gtcggatcac cgataacgtt ggcaaatcat
     1441 agggaaattg gtcaaaaata gcgatgaaaa aggcaaccat cgggttgact tttttgcgac
     1501 acattatcgt taacgtggct tttgccttcg gcgccagcga cccttagcgc tgttgcaaca
     1561 gcccacgcag cagcgccagt cgcaacgccc tggcctgcct tgcagcgcgg cgttcgggca
     1621 tttgcagaag acttgtcgac tgcgccgggc actggcgcag cgccgcaccg gctcagaact
     1681 ttctgacttc aatctcgttc ttttgcagcg cgtagcccat ttgccgtggc gagattttca
     1741 gcagccgggc ggccttggcc tgcacccagc cgcagcgctc catggcccag atcaggcgtt
     1801 cacgctcgcc gtcgggcttg ccatcggcat ctggcagcgg tgcgtcaagg tcagcgggcg
     1861 cggcttcacc cggcgcttcg ccacctgccg ctgtcgccga cgctgtcgct gccactggtg
     1921 gcggcggtgg gaagttgcca gcgggcagcg gattcagcgg cacttccagc accggaatgt
     1981 ccgccaggcg ctgcggcgtg accgcatcga cccggtccat gtggtgcagc acctgcgtca
     2041 ggcaatggtt gccctggcac ggaaaatcga tatcgagaat gacatcgcca tgcgccatgg
     2101 tggcggtacg ctcgatgcag ttttccagtt cgcgcacatt gccgggccag tagcaactgg
     2161 tcagcacccg catggcggca gggctgagct gcgtgttgcg ggcgttttcg gcgttgaatt
     2221 tgtccagaaa atgctgcgcc atcaccggaa tgtcttcccg ccgctcgcgc agtggcggca
     2281 actggatgct gaccacgttg atgcggtagt acagatcggc gcgaaattcg ccagcctgca
     2341 ccatgcgctc cagattgcgg ttggtggcca ggatcagccg cacatcgacc ttcaccacgg
     2401 tcgagcctcc gacccgttcg aattcgcgct cttgcagcac ccgcagcagc ttggcctgaa
     2461 aggcggccga gatgtcgcca atttcatcca gaaacagggt gccgccatgc gacatttcaa
     2521 agcgtccctt gcgttggccc tgggcgccag tgaaggcccc tttttcgtgg ccaaacagct
     2581 cgctctccag cagcgactcg gtcaacgcgg cgcagttgac ccgcacaaag gcctgatgct
     2641 tgcgcggtga caggttatgc acggcgcggg caatcacctc tttgccggtg ccgctctcgc
     2701 cacgcagcag caccgtggtg cgtgccggtg ccaccttgtg gacctggtca aacacc
//
LOCUS       DAOSLL010000629         2749 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_31997_length_2749_cov_3.628062, whole genome shotgun sequence.
ACCESSION   DAOSLL010000629 DAOSLL010000000
VERSION     DAOSLL010000629.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31997_length_2749_cov_3.628062"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            25..789
                     /locus_tag="PLB25_20085"
     CDS             25..789
                     /locus_tag="PLB25_20085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43905.1"
                     /translation="MTVHLYLSLIPEALIASMLPPEDFGQYYSTGHHFKSKGQAMFFE
                     IDPAFRCDALNIKEAVARCVPHSDGEPRHSVYASVYRVLEHIPVSAIGKLYLCTAYGQ
                     ALAIERAEMIKNDQPCLHLYQDLAPVNSLIVSSLNPAEVYNSVTVSPSKFIQFPALCF
                     VELYLGELAANPEFGSTNDLPYSFMHHLREALITLHPEEKISKLVHRVHSLEIPYRMV
                     KSGFYVGNGPDLAFYAMPSHQEMRKNHAKWWRLANS"
     gene            complement(871..1692)
                     /locus_tag="PLB25_20090"
     CDS             complement(871..1692)
                     /locus_tag="PLB25_20090"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HOE43906.1"
                     /translation="MSKTLVLHLVSHASGELIEMLARNAVAQLEGVHVERRLWKRVRR
                     MGQLPEILAAVADSPGFVLHSISDTDIREAIESACDRLGVPCQFALEPLIGQLAEYSS
                     APVQVHTSSGSAFDEDYYVRVEAMKYTLAHDDGVASDDLTDADVIILGVSRATKTPTC
                     MYLASRGIKAANVPLVPGIPIPAALVSPKAPLVVGLIVDPARLSLIRSARLTSLNQHE
                     NTAYADFDALRRELLAARRLFILHGWPIIDVTNRSIEQTSAMIIEMLEARAAHQP"
     gene            complement(1732..2484)
                     /locus_tag="PLB25_20095"
     CDS             complement(1732..2484)
                     /locus_tag="PLB25_20095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE43907.1"
                     /translation="MDIWECSAVVTGAASGLGAETARYLAEAGAKVTVIDIDGIGVQK
                     IADEIGGLAVHCDVADSLSLENALATARQAHGAARVLVHCVGRGHSAPIVGPNGPMPL
                     EDFRQLVEVNLISTFNMMRLAAADMAQLDPMENGERGVILSTASVSAYEGQSGEAAYS
                     GSKGGVISMILPAARELGPLGIRVVGIAPGLFGTQTLFNMEKNLDSRVAHQIPFPHRF
                     GDAAEFAMLVIHVLNNVMINGTVLRLDGGYHR"
BASE COUNT          650 a          761 c          700 g          638 t
ORIGIN      
        1 gttagatcga aagagcatta aatcatgacc gtccaccttt atttgtcatt gatacctgaa
       61 gcgctaattg catcgatgtt gccgcctgag gactttggtc agtattactc taccgggcat
      121 catttcaagt ctaaagggca ggcgatgttc tttgaaatcg accccgcatt tcgctgtgac
      181 gcgctcaaca tcaaagaggc ggtggctcgc tgtgtgccgc attcagatgg cgaaccaaga
      241 cactctgtgt atgcctcggt ctatcgagtg ttggaacata ttccggttag cgccattggc
      301 aaactttatt tgtgtacggc ctatggtcag gcgctagcca tcgaacgggc tgaaatgata
      361 aaaaacgacc aaccgtgcct gcacctctat caggatctgg ctccggtcaa tagtctgatt
      421 gtcagttcac tcaatccggc cgaggtatat aacagcgtta cagtttctcc atccaaattc
      481 atccagtttc cagccttgtg ttttgttgaa ctctatctcg gtgaattggc cgcaaaccca
      541 gaattcggct ctaccaacga cctcccgtac tctttcatgc accatttgcg cgaagcgctg
      601 atcaccctgc atccggagga aaaaatcagc aaattggtgc atcgagtcca ttctttggag
      661 attccgtacc gcatggtcaa gagcggcttt tacgtcggta acggtccgga tctggcgttt
      721 tatgctatgc catcgcatca ggaaatgcgt aagaaccatg ccaagtggtg gcgcttggca
      781 aattcttgaa tctgcccatt tgttcaccga aaagttaatc agttgcgtgc cttggtctga
      841 aataaggcga tatccgcaca gtttgcagag ttacggctga tgagcggcgc gcgcctccaa
      901 catttcgatg atcatcgcag aggtttgctc aatggagcga ttggtgacat caatgatcgg
      961 ccagccgtgt aagatgaata agcggcgcgc ggccagtaac tctctgcgca aggcatcaaa
     1021 gtcagcatag gcggtatttt catgctggtt cagcgatgtc aagcgggctg atcgaatcag
     1081 ggacagtcgt gccgggtcga cgatcaatcc gacgacaagt ggtgccttcg gactcaccaa
     1141 cgccgccgga attggtatgc cagggaccaa tggcacattg gcggccttga tgccacggga
     1201 agccaagtac atgcaggtcg gtgtcttggt cgcccgagag actccgagga tgatcacgtc
     1261 agcatcggtc aaatcatcac tggctacccc gtcgtcgtgg gctagcgtgt acttcatggc
     1321 ttcaacccgg acgtaatagt cttcgtcaaa ggcgctgcct gaactggtgt gaacttgcac
     1381 cggcgcactg gagtattcgg caagctgccc gatcagcggt tcaagcgcga actgacatgg
     1441 aacacctagc cggtcacacg cagactcaat ggcttcgcga atatcggtgt cgctgatact
     1501 gtgcaggacg aaaccaggtg agtccgcaac agccgccagt atctctggaa gttggcccat
     1561 gcggcggacc ctcttccata gtctgcgttc aacgtggacc ccttcaagtt gtgccacagc
     1621 gttgcgggca agcatctcaa tcaactcgcc cgaagcatgc gagacgaggt gtaacaccag
     1681 agtcttgctc acggaggatt cctcttgttc gatcatatat ctttgcccga attaccggtg
     1741 gtagccgcca tccaaacgca gaactgtgcc gttgatcatg acgttgttaa ggacatgaat
     1801 aaccagcatg gcgaactcgg ctgcatcacc gaaacggtgc ggaaaaggta tctggtgcgc
     1861 gaccctcgaa tcgaggtttt tttccatgtt gaatagcgtc tgcgttccga ataatccggg
     1921 cgcaattcca actacacgaa tgcccagggg gccaagttct cgggctgcgg gcagtatcat
     1981 gctgatgaca cctcctttgg agcctgaata tgcagcttcg ccgctttgcc cttcgtaggc
     2041 cgaaaccgag gctgttgaca atatgacacc acgttcgccg ttttccattg ggtccagttg
     2101 ggccatgtca gcagcagcta gccgcatcat gttgaaggtc gagatcagat tgacctcaac
     2161 cagttgacga aaatcttcca gtggcatcgg gccgttgggg ccgacaattg gcgcagaatg
     2221 cccgcgtccg acacagtgga ccagcacacg agccgcacca tgcgcctgcc gcgccgtagc
     2281 cagcgcattc tccaacgata gcgaatccgc cacatcacaa tggacggcaa gtccaccaat
     2341 ctcatctgcg attttttgca cgccgatacc atcgatatcg attaccgtaa ccttggcacc
     2401 tgcttcagct agataacgcg ccgtctccgc tcccaacccc gatgccgcgc ctgtgaccac
     2461 tgccgaacat tcccagatat ccatcaaata ctcctcgcct cgacggtagt tacgccaagg
     2521 gcaaaacaac aaatcacagc ttgcattttt tgctgctgcg gccctttgca aggtgtcgca
     2581 ggcacctcag tccagcgttg ctcgatacat ctcacaagca cgggaatacc tattggctcg
     2641 aagaatagga aggaactcgc tgccaagcca aaccattgct ttcaagagag tcacaacagc
     2701 gcaagcacga gcttgcgtgc gaatcaaata tcttcaggca ccattttga
//
LOCUS       DAOSLL010000630         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_32043_length_2745_cov_4.510037, whole genome shotgun sequence.
ACCESSION   DAOSLL010000630 DAOSLL010000000
VERSION     DAOSLL010000630.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32043_length_2745_cov_4.510037"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(328..1323)
                     /locus_tag="PLB25_20100"
     CDS             complement(328..1323)
                     /locus_tag="PLB25_20100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HOE43908.1"
                     /translation="MLSDQNQKKLVVLGTGGTIAGIASKPSDNIGYTAAQVGVAALLD
                     AVPGLNGVLAGCQLLSEQVAQVDSKDMDFDIWKKLAQRVHHHLSQTDVQGIVVTHGTD
                     TLEETAYFLQAVLPPSLLNGKPVVLTCAMRPASALAPDGPQNLLDAVTVALAAGAHGV
                     VVVCAGAVHTAQDVQKNNTYRLDAFSSGDAGVMAYVEEGQLRLLRNWPVALDTYALAA
                     IDIIVSCAVWPRVEVVMSYAGCGGQLVDALLRPSVGVTPVRGLVVAGTGNGTLHRDME
                     AALRRAQAQGVSVVRATRCPNGRVLPTESSEFPHSRGLSPVKARIALMLELMQSS"
     gene            1443..2120
                     /gene="lexA"
                     /locus_tag="PLB25_20105"
     CDS             1443..2120
                     /gene="lexA"
                     /locus_tag="PLB25_20105"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HOE43909.1"
                     /translation="MHIRPKLTDRQQQILDLIQSAITRTGAPPTRAEIASELGFKSAN
                     AAEEHLQALARKGAIELVSGTSRGIRLRSDMMRSINESRSKQFASPLASLAQLALPLI
                     GRVAAGSPILAQEHVDKTYYVESSLFQRKPDYLLKVRGMSMRDAGIMDGDLLAVQTTR
                     DAKNGQIVVARLGDEVTVKRFMRNKNDLIELHPENPDYQTIVVEPGEPFEIEGLAVGL
                     IRNTMLM"
     gene            complement(2279..2554)
                     /locus_tag="PLB25_20110"
     CDS             complement(2279..2554)
                     /locus_tag="PLB25_20110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43910.1"
                     /translation="MALCLACSQAIKLGAYVSHAARYTQTTDLGRGEASLDDPQYPQR
                     SPSAGPIAGARSDAWHLGVGRAGAAMGCGCHRADVALILGLLVGSGH"
BASE COUNT          622 a          875 c          731 g          517 t
ORIGIN      
        1 tgacagccac gacccgcaaa cgccccacct tgcacgccgg gcaaaggtca atgtcggaaa
       61 ctgtcaatgt cggccacgcg cgccatgaag gcctgcgcac actgcagtgc cagcgcgttg
      121 ggcgcaggct tctgcaaagg ctaaatccgc gcggcaccat cgcatcgatc catccgttga
      181 caacacggtg gcgtggcaaa tctccgtcaa aaccggggaa catggcgcag cctgaagact
      241 tgtcccaatt ggcctcttgc gcaagtcagg cgcaaacata aacgtggtgg tggcctgcgc
      301 gacccgccac cgcgtcagcg gcttagttca actggactgc atcagttcca gcatcagtgc
      361 gattctggcc ttgaccggcg acaagcctcg ggagtgggga aactccgagc tttcggtggg
      421 aaggacccga ccgttggggc aacgggtggc acgcaccacg ctgacccctt gagcctgcgc
      481 gcgacgcagc gctgcttcca tgtcccgatg aagagtgccg ttgccagtgc ccgccacaac
      541 caggccgcga acaggtgtga cgccgacact tgggcgcagc agggcatcaa ccaattgacc
      601 gccgcatccg gcatagctca tgaccacctc gacacgtggc cagacagcgc aacttactat
      661 aatatcaata gctgctaagg catacgtatc tagcgctaca ggccaatttc ttaacaacct
      721 gagttgacct tcctcaacgt aggccatgac gcctgcatcg cctgagctga aggcatcgag
      781 ccgataggtg ttgttttttt gcacatcttg cgcggtgtgg accgcaccgg cacacaccac
      841 caccacgcca tgcgcaccgg ctgccagcgc cactgtcacc gcatccagca gattttgcgg
      901 cccgtctggc gcgagcgctg acgctggccg catggcacag gtcagcacca ccggcttgcc
      961 attgagcaag ctcggcggca gaacggcttg caaaaaatag gcggtttcct ccagcgtgtc
     1021 ggtgccatgg gtcaccacga tgccttgaac atcggtttgc gaaaggtggt gatgcacgcg
     1081 ctgcgcgagc ttcttccaga tgtcaaagtc catgtccttg ctgtccacct gcgccacctg
     1141 ctcgcttagc aattgacagc ctgccagcac cccgttcaac cctggcacag catcgagcaa
     1201 cgccgcgacc ccgacctgcg ctgcggtata gccaatgttg tcggatggct tgctggcaat
     1261 gccggcgatc gtgccaccag tgcccagcac caccaatttc ttttgatttt gatcagatag
     1321 cacttgcagc ctttcaaaaa ctggttaaaa atacagttac tggatatgaa gacagtgaac
     1381 gcacttatcc agcgccctgt gcaccccact ggcctgccac ctaactgtta accggagtca
     1441 tcatgcacat cagacccaaa ttgacggatc gtcagcagca aatccttgat cttatccaga
     1501 gcgccatcac ccgcactggc gcgcctccta cccgcgctga aatcgcctca gagctagggt
     1561 tcaagtcagc caatgccgcg gaagagcacc tgcaggcact tgctcgcaag ggcgccattg
     1621 aactggtgag tggcacctca cgcggcattc gccttcgcag tgacatgatg cgatccatca
     1681 acgagtcacg cagcaaacag ttcgcatcgc cattggccag cctggctcaa ttggccctgc
     1741 cgctgattgg cagggtggca gcaggttcac ccatattggc gcaggagcat gttgacaaga
     1801 cctactacgt cgaaagcagc ctgtttcaac gcaagcctga ctacctgctg aaagtccggg
     1861 gcatgtcaat gcgtgatgct ggcatcatgg atggcgatct gttggcggtg caaaccaccc
     1921 gtgatgcaaa aaacggccag atcgtcgtgg cccgcctggg cgatgaggtc accgtcaagc
     1981 gcttcatgcg taacaaaaat gacctgattg agttgcaccc cgaaaacccc gactaccaga
     2041 ctatcgtcgt tgagcccggt gaaccctttg agatagaagg cttggcggtt ggcctgattc
     2101 gaaacaccat gttgatgtag cacgctggca tcaccagaca ttgccgccac ccgtcgaacc
     2161 caggccaatt gccaggcgac tggccatttg ccaagccata tcagtcaagg attgcaccat
     2221 gaacgcccaa cacagcagcg cgcacagcac cactcccctc agtgcccttt ggcactggct
     2281 aatgaccgga cccaaccagc aagcccagaa tcaatgccac gtcggcccgg tggcaaccgc
     2341 accccatggc cgcaccggca cgaccaaccc ccaaatgcca ggcatcgctt cttgcaccag
     2401 caatcggccc ggcactcggc gacctttgcg ggtactgcgg gtcgtcgagg ctggcctcgc
     2461 ccctgcccaa atcggtcgtt tgcgtatatc gggccgcatg gctgacgtat gcgccgagct
     2521 tgatcgcctg gctgcatgcg aggcaaagtg ccactgaaca cgcagtaacc gactcagcaa
     2581 acgcgcttgc cgcatcgtgt ttggcgactg caacccatat tggttaattt catcaccaag
     2641 gaaatgcgaa cccggtaaca ccaaggcaca attggcggca tgaatattgt gatacttgac
     2701 gactaccaag atgccgtaag aaagctggca tgcgcagcca agctt
//
LOCUS       DAOSLL010000631         2745 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_32048_length_2745_cov_3.590706, whole genome shotgun sequence.
ACCESSION   DAOSLL010000631 DAOSLL010000000
VERSION     DAOSLL010000631.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32048_length_2745_cov_3.590706"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1436)
                     /locus_tag="PLB25_20115"
     CDS             complement(<1..1436)
                     /locus_tag="PLB25_20115"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE43911.1"
                     /translation="MTRFPGYTPVEQIFIGSRSRIVRAVRDIDGVRVVLKVPVSDQLP
                     LRDLLRFEHEYRVLESLDLKHVIKVHALVPHGAGFLVVEEDFGAQDLAHFLSEKSLCP
                     NDFYPIAQAMAEGLAELHANLVLHKDLHPGNVLIEPLSGLVKLTDFGLSTLIEDETPD
                     TLAAEMIEGSLAYISPEQTGRMNYPVDSRSDLYALGVCYYRMLTGQLPFAATDALEMM
                     HAHIAKQPVEPRAVASGVSQELSALVMKLLAKEPRQRYQSARGVVADLQALAAASRLG
                     GVVADFVPGAKDVSTRFQVSRTLYGRESQVAQLLSAFDRVALGRTELLLVAGYSGVGK
                     TALVQEVHRSLAHCKGRFLSGKHDQFRRDFPFSALIQAFGQLMRHILAERDERVALWR
                     KALLEELGSNARILVDVLPEAAILLGPQEAAPELGPVETKNRFVLTLQRFLGVFARSQ
                     HPLVVFVDDLQWADNASLQLMRALCRDTA"
BASE COUNT          651 a          812 c          723 g          559 t
ORIGIN      
        1 gcggtatcgc ggcacaaagc ccgcatgagc tgcaaagacg cgttgtcggc ccattgcagg
       61 tcatccacga aaacgaccaa ggggtgctgc gagcgggcaa agacacccaa gaatcgttgt
      121 agcgtcagca caaaccggtt cttggtttca acaggcccca gttcgggcgc tgcttcctgg
      181 gggccaagga ggatcgcggc ttcaggcagc acatccacca ggatgcgggc gttgctgccc
      241 agctcttcga gcaacgcctt acgccacagg gctacacgct cgtcgcgctc tgccagaatg
      301 tggcgcatca gttgcccgaa ggcctgaatc aaggcagaaa acggaaaatc cctccgaaac
      361 tgatcgtgct tgccgctgag aaaccgcccc ttgcagtggg ccagacttct atgaacttcc
      421 tgcaccaggg ccgtcttgcc tacgcctgaa tagcctgcaa ccaaaagcaa ctccgtgcgc
      481 ccgagcgcca ctctgtcaaa tgcggacaac agttgtgcca cttgactttc ccgtccatac
      541 agggttcgcg acacctgaaa ccgggttgac acatccttcg cccctggaac aaagtcggcc
      601 accaccccgc ccaagcggct ggctgcagcg agtgcctgca ggtcggcgac gactccccgt
      661 gcgctctggt agcgctgccg tggctccttg gccaggagct tcatcaccag cgcagacaac
      721 tcttggctca cgccggatgc cacggcccgc ggctccactg gttgcttggc gatgtgtgca
      781 tgcatcattt ccaaggcatc ggtcgcggcg aatggcaact gtccggtcaa catgcggtag
      841 tagcaaaccc ccaaggcgta gaggtcagaa cgggagtcga ccggataatt catccggccc
      901 gtctgctcgg gcgagatata ggccaggctg ccttcaatca tttccgcagc aagcgtatcc
      961 ggggtctcgt cttcaatcag cgtggaaagg ccaaagtctg tcagcttgac aagtcctgaa
     1021 agcggttcga tcaacacatt gcccggatgc aggtctttgt gaagcaccaa gttggcgtgc
     1081 agttcggcca acccctcggc catggcttgt gcaatcgggt aaaaatcgtt tgggcaaagt
     1141 gacttttccg acagaaagtg agccagatcc tgggcaccaa aatcttcctc tacgacgaga
     1201 aacccggcgc catggggaac cagcgcatgc accttgatga catgcttcaa atcaagcgac
     1261 tccagcactc ggtattcgtg ctcaaaccgc agcagatcgc gcaggggaag ttggtctgaa
     1321 accggcacct tcaacaccac ccgcaccccg tcgatatcgc gtacggcccg cacaatgcgg
     1381 ctgcgactgc ctatgaaaat ctgctctaca ggggtatagc cggggaatcg ggtcatgcct
     1441 ggactctggg acgaatggac caagtatcgt accctgtgca catccaccga agttaagccc
     1501 atgtcatgcc ttattggcaa tgctcggatg gccttcctgc cgctgacctt gaaaccgtgc
     1561 ctgcaaaaaa attttttgcc cactgaaata ggccaattaa ccgtacgagg cgcagtatca
     1621 cgcccaggaa caggcttcga accttgtgca aacccgcaat ttcctgcccg cgtggcaggt
     1681 tcactatcat aaggcaccgt cgacagagcc agtagcacag cgccgggtgc gtcgagcgca
     1741 ggtctaccag gcggcgaccc tgaaaaatcg ccgcctcgat cagtcaccaa ttgatgcgtt
     1801 gatcaagctc ggcgtctgga gccggttacg cgaccccgtc ccagtcagtc gaccagtctg
     1861 gctcaatgta caccccgtca tccagccgcg catcaccacc atcctttcaa agcggtggcc
     1921 cacgtgccgg ggacaaccca tagccaacgt ccagtctgga ggaagcaaat cgcaaagtgc
     1981 tactaccgct atgggtcttt atgcatagga ctcagttatg catagtattt tcatcgacag
     2041 tgctggaaac cgcatgaacg ttgacttttc ggcaatcttc agctcaaaga ctcctcataa
     2101 attcgactat tcaccaaggc ccgcctttgc aactagaact tcaggagcca acgcaagagc
     2161 catctcggga aattctgcgt ccgcaggcca ttggacagaa tcagtagcct tttgaaccca
     2221 tgccaagcaa attccataac cgcgccttgg agaacatttt ctgaaactga cgaggcgttg
     2281 atattatgta gaagctaaat ctggcttgcg gcaatgtttg cctatgacag gtgcatcgct
     2341 gacacggtag ctattcataa ctgagtccta tgcataaaga cccttatgta gagctataca
     2401 taagggtctg atgcgggtat tggagaaaaa aagcagattt ccacggccac cggctcctgc
     2461 agacccaaac tagccattca ccaaaattgc taaacaacca tcggaaagtc gaaagcgggt
     2521 gctcggggtt gcgaacaggt cgctccaaag ccgccgctga cgatttctgc tatttgcggg
     2581 gagtaaactg ttcgctaagc atgtgcgacc cgccaacgac caggttgccg acgggacatc
     2641 gcacctgctg aggttacatc ttgggaaggg tcagaatgga ctccgtcaaa aaatggtggt
     2701 ctttagcgtt tgtgcgaagc ctgccggtgt tcttgctgtt agcct
//
LOCUS       DAOSLL010000632         2737 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_32171_length_2737_cov_4.141312, whole genome shotgun sequence.
ACCESSION   DAOSLL010000632 DAOSLL010000000
VERSION     DAOSLL010000632.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32171_length_2737_cov_4.141312"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /locus_tag="PLB25_20120"
     CDS             complement(<1..1158)
                     /locus_tag="PLB25_20120"
                     /inference="COORDINATES: protein motif:HMM:NF017658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HOE43912.1"
                     /translation="MRTFAANPVHRPSRLVCALRLAGLVTVTALAAQNPALAQIRTDS
                     SLGQSARTLVGPAYVIPQSLGKLSGNNLFHSFQTFNLDTNESANFTTTTAGLSNVISR
                     VTGGTLSQINGLISLTAANATPNFFFINPAGVTFGAGASIDVPAAFHVSTANYLKFAD
                     GKFYADASSASTFSSAEPAAFGFLGSSRATIQIKDSVLYTPSGGISLVAGDLSIDHAG
                     LWTGSGDIRAAAMGAGPAEVSLRGTLPIASGRLAMNNGSNLYSGATAQSAGGNIEIAA
                     GDASLQGGALVVTDSSSAGPAGSITAELGSLVIDGRSATSSTGITSLASMYDVGAGGD
                     VAVTARGAVKILGGGMITSDTYGMGAAGNVAVKAGSLLLDGSGHANAARITS"
     gene            complement(1155..>2737)
                     /locus_tag="PLB25_20125"
     CDS             complement(1155..>2737)
                     /locus_tag="PLB25_20125"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HOE43913.1"
                     /translation="AEALALTQQALEQAALMPASAIADVLMALESRQGRLFTLLGQPS
                     RALAAYQRAADQAELLRPDLPIEFEDGRSSFRQTLEPIYLGLVDALLTAADAQSAAAS
                     ENYLRRALDATELLKQAEMQDYLGDRCTVDTVKGGSATVIAPETAVLYPIIFPDRVEL
                     LLQTQDSIARFGSRVAGALVRSTASSLAKDLRNGELDFVRQSRQLYDWLLRPLESFLT
                     AHQINTLVLVPDGALRLVPVSALHDGKQFAIEKYAISTVTGLSMTNTNASSVRNYVAL
                     VAGASHFGPVVDKLGLTKLGQALAADVAGQDQGRGLARSRMLRAPAHTRSTDPLATPA
                     NQVRFMREALALPGVAKEIESVGRILPGTSLLDAGFTVDAFSNAAESGAFRILHVASH
                     GVFGGAADSSYIMAYDDLLTLDGLQSLLANEQVRKRPIELLSLSACETAEGNDRAPLG
                     ISGAAIKARAKSVLGTLWPVDDEAAVRVMEQFYRNLAQSHLSKAQALQAAQVELLQDR
                     EFAHPFFWAPFVLIGNWL"
BASE COUNT          558 a          838 c          808 g          533 t
ORIGIN      
        1 gctggtgatg cgggcagcgt ttgcatgtcc ggatccatcc agcaacaacg atccagcctt
       61 gacggccaca ttgccagcgg cacccatgcc gtaggtgtcg gaggtgatca tgccgccgcc
      121 caaaatcttg accgcgccgc gtgcggtcac cgccacatcg ccgccagcgc caacgtcgta
      181 catggatgcc aggctggtga tgccagtcga gcttgttgcg ctacgcccat cgatcaccag
      241 actgccgagt tctgccgtga tggaccctgc cggaccggcg ctgctgctgt cagtcaccac
      301 cagtgcacca ccctgaaggc tggcatcacc cgctgcgatc tcaatattgc cgccggcgga
      361 ttgtgctgtt gcgccgctgt agagattact gccattgttc attgccagac ggccactggc
      421 gattggcagt gtaccgcgca ggctcacctc ggccgggccg gcacccatgg cagcagcacg
      481 gatgtcacca ctgccagtcc acaacccggc atggtcgatg ctcagatcac ccgccaccag
      541 tgaaatgccc cccgaggggg tgtacaacac gctgtccttg atctgtatcg tcgcccggct
      601 actgcccaga aaaccaaacg ccgccggttc ggcactggaa aacgtgctgg cgcttgacgc
      661 gtctgcgtaa aacttgccgt ccgcaaactt caggtaattg gcggtgctga catgaaatgc
      721 cgctggcaca tcgatactgg caccggctcc aaaagttacc ccggccgggt tgatgaagaa
      781 gaaattgggt gtggcgttag ccgccgtgag tgaaatcagg ccattgatct gcgacagcgt
      841 gccaccggtg acccggctga tgacgttgct caggcccgcc gtggtggtgg tgaagttggc
      901 cgattcgtta gtatcaagat tgaaggtctg gaagctgtga aacaggttgt tgccagagag
      961 cttgccgagc gactgcggta tgacgtaggc cgggccgacc agtgttcgag ccgactggcc
     1021 cagactgctg tcagtgcgga tctgggccag tgcggggttt tgcgcagcca aagcggtaac
     1081 cgttaccagc ccggccagcc gtagcgcaca gaccagccgt gacgggcgat gaaccgggtt
     1141 tgccgcaaat gttctcataa ccagttacct atcagtacaa acggtgccca aaagaaagga
     1201 tgggcaaatt ccctgtcttg aagcaattca acctgcgctg cctgcaaggc ctgtgccttg
     1261 gacaaatggc tttgcgccag attgcggtaa aactgctcca tcaccctgac tgccgcctca
     1321 tcgtccaccg gccaaagggt tcccagcaca ctcttggccc gggccttgat ggcggccccg
     1381 gaaatgccca agggcgcgcg gtcattgccc tcggctgttt cgcaggccga caggctcagc
     1441 aattcaatcg ggcgtttgcg aacctgctca ttggccaaca gcgactgcaa gccatccagc
     1501 gtcaaaagat cgtcataggc catgatgtaa ctcgaatctg ccgcaccgcc gaacacgcca
     1561 tgcgatgcca cgtgtaatat gcgaaatgca ccagactcgg ctgcattgct gaaggcatcg
     1621 acggtaaagc ccgcatcgag caagctggtg ccgggcaaaa tgcgaccaac ggactcaatt
     1681 tctttggcga caccgggcaa agccagtgcc tcgcgcataa accgtacttg attcgccggt
     1741 gttgccagcg ggtcggtgct cctggtgtgt gctggtgcgc gcagcatgcg gctgcgcgcc
     1801 agaccgcgac cctggtcctg cccggctacg tcggcggcca gcgcttggcc caatttggtt
     1861 aagcccaatt tatccaccac cggcccgaaa tgggaggctc cggccaccag cgccacatag
     1921 ttcctgacag atgaggcatt ggtattggtc atggacaatc cggtgaccgt gcttatggca
     1981 tacttttcga tggcaaattg cttgccgtcg tgcaaggcac tgacgggtac cagcctgagc
     2041 gcaccgtcag gcaccagcac cagggtgttg atctggtgcg ccgtgagaaa tgactcaagt
     2101 ggccggagca accagtcata gagctgcctg gattgccgaa cgaagtccaa ctcgccgttg
     2161 cgcagatctt tggccaggga actggccgtg ctgcgcacca gggcaccggc aacacggctg
     2221 ccaaagcgcg caatgctgtc ttgtgtttgt aacagcaact caacccgatc cggaaaaatg
     2281 atcgggtaca gcaccgctgt ttcaggcgca ataacggtgg cgctgccacc cttgacggtg
     2341 tcaaccgtgc accggtcacc cagatagtcc tgcatctccg cttgcttgag caactctgtt
     2401 gcgtccaggg cccgacgcag gtagttttcg ctcgctgccg cgctctgggc atcggcggcg
     2461 gtcagcagcg catcgaccag accgaggtaa atcggttcca gcgtttggcg aaaagaagat
     2521 ctgccgtcct caaattcgat aggcaggtcc gggcgcagca attcagcttg gtctgcagcg
     2581 cgttggtaag cggccagcgc tcggcttggc tgacccagta gggtgaacaa tcggccctgg
     2641 cgggattcga gtgccatcaa aacatcagct atcgcgcttg caggcatcag cgccgcctgt
     2701 tcaagcgcct gctgcgtcag ggccagtgct tcagcat
//
LOCUS       DAOSLL010000633         2730 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_32296_length_2730_cov_4.899439, whole genome shotgun sequence.
ACCESSION   DAOSLL010000633 DAOSLL010000000
VERSION     DAOSLL010000633.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32296_length_2730_cov_4.899439"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1056
                     /gene="earP"
                     /locus_tag="PLB25_20130"
     CDS             <1..1056
                     /gene="earP"
                     /locus_tag="PLB25_20130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HOE43914.1"
                     /translation="ICWRLAVNLAQRGQTVRLWVDQPGALAWMAPNGAPGVQVLPWVS
                     PAAMVGIKPGDVLVEAFGCDIDLEFIAAYADCIRAEGRKCVWINLEYLSAEPYAQRCH
                     GLPSPVMSGPGKGLIKHFFYPGFVPGTGGLLRETDLLTRQAGFDRAAWLAVQHIRWQG
                     ERLVSLFCYEPTGLAQLLDTLAEAERPSLLLVTPGRAAAAVKPLVQDKKGLQTNWNKR
                     GQLSISYLSKLSQTDYDHLLWACDVNFVRGEDSLVRALWAGQPLVWQLYPQHDGAHQA
                     KLAAWLDWLQAPDGLRAFHAAWNGLAATTTAAHRPQALPLDDLPLWQQTVAQARQRLL
                     GQPDLTSALLGFAAKTN"
     gene            1164..1718
                     /gene="efp"
                     /locus_tag="PLB25_20135"
     CDS             1164..1718
                     /gene="efp"
                     /locus_tag="PLB25_20135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012346962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HOE43915.1"
                     /translation="MKIAQEIRAGNVIMNGKDPMVVLKTEYSRGGRNSATVRMKLKSL
                     IANFNTEIVYKADDKLDQVILDKKDCTYSYFADPMYVCMDSEYNQYEVEAENMGDSLS
                     YLEDGMPLEVVFYDGKAISVELPTSVVREITWTEPAVKGDTSGKVLKPAKLATGFEIG
                     VPIFVAQGDNVEIDTRTGEYRKRV"
     gene            1799..2674
                     /locus_tag="PLB25_20140"
     CDS             1799..2674
                     /locus_tag="PLB25_20140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HOE43916.1"
                     /translation="MEHTQSLPEHITEHRLADAWAELLKHPANATVVHPNAYRLAFAD
                     PEFMLRRDTRGIRIQLEMLKPDLDQAEQRIDNTVVVFGSARFCSPETAQQAMAQAQFS
                     GDAQAIALAQRQLRNAHHYDQARLFARLVAGYSAALDVNERLYICTGGGPGIMEAANR
                     GAHEMGAPTVGLNITLPHEQHANPYVTPSLSFKFHYFALRKMHFMMRAKALVAFPGGF
                     GTLDELFEVITLVQTRKSQPVPIVLFGSDYWRRLLNTEVLLEEGVVSSEDLELFHYVD
                     EPQAAWGIIKGFYGL"
BASE COUNT          547 a          829 c          800 g          554 t
ORIGIN      
        1 atttgctggc ggctggcagt caacctggca caacgcggcc agaccgtgcg gctgtgggtg
       61 gaccagccag gagccctggc ctggatggct ccgaacggcg cgcccggcgt gcaggtgctg
      121 ccgtgggttt cccctgctgc aatggtcggc atcaagcccg gcgacgtgct ggtagaagca
      181 tttggttgcg atattgatct tgaattcata gctgcttacg cagactgcat aagggctgaa
      241 ggccgaaaat gcgtgtggat caaccttgag tacctgagcg ccgagcctta tgcccagcgc
      301 tgccatgggc tgccttcgcc ggtgatgagc ggaccaggca agggcctcat caagcatttc
      361 ttttaccctg gcttcgtccc tggcactggt ggcctgttgc gagaaactga tctgctcacc
      421 aggcaagccg gttttgatcg cgccgcctgg ctggccgtgc aacacatccg ctggcagggc
      481 gagcgcctgg tgtcgctgtt ttgttacgag ccgaccgggc tggcgcagtt gctcgacacg
      541 ctggccgagg ccgagcgccc gagtctgctg ctggtcacgc cagggcgtgc ggccgccgca
      601 gtcaaacccc tggttcaaga caaaaaaggc ctgcagacca attggaataa gcgcggacag
      661 ctatcaattt catatctttc gaaactctcc cagactgact atgaccacct gctgtgggct
      721 tgtgacgtca attttgtacg gggtgaggac tcgctggtgc gggcattgtg ggccgggcag
      781 ccgctggtct ggcagcttta cccccagcat gacggtgccc atcaggccaa gctcgctgcc
      841 tggctggact ggctgcaggc accagatggc ctgcgtgcct ttcatgctgc ctggaatggc
      901 ttggcggcca caaccactgc ggcgcaccgg ccgcaggctt tgccgctgga tgatcttccg
      961 ctctggcagc aaaccgtggc acaggcccgc caacgcttgc tgggccaacc cgatctgacc
     1021 agcgcgctgc ttggttttgc tgcgaaaacc aactaaaata gcgggctttg cggcttgggg
     1081 ttgaattttc attcaatttt tcggtcgccc tcgccgcctg ctgcgcggcc tacttttcac
     1141 cttatcgctt ttaagtttct tttatgaaaa tcgctcaaga aatccgtgcc ggcaacgtca
     1201 tcatgaacgg caaagacccg atggtggtcc tgaaaaccga atacagccgt ggcgggcgca
     1261 actcggccac agtgcgcatg aagctcaaaa gcctgattgc caacttcaac accgaaattg
     1321 tttacaaggc cgacgacaag ctcgaccagg tcattctgga caaaaaagac tgcacctatt
     1381 cgtactttgc cgaccccatg tacgtctgca tggacagcga gtacaaccag tacgaagtcg
     1441 aggccgaaaa catgggcgac tcgctgagtt atcttgaaga cggcatgccg cttgaagtgg
     1501 tgttttacga cggcaaggcg atctcggtcg aacttccaac cagcgtggtg cgcgaaatca
     1561 cctggaccga gcctgcagtc aagggcgaca cctccggcaa agtgctcaaa cccgccaagc
     1621 tggccaccgg tttcgagatc ggcgtgccga tttttgtggc gcaaggcgac aatgtcgaaa
     1681 tcgacacccg caccggcgaa taccgcaagc gcgtctgatc aacttgggcc ggcccgccag
     1741 ccggttggat tgaaaggctt cctggtgaag cctttttttt ggcccacaat cacgccccat
     1801 ggaacacacc caatcgctgc ccgaacacat caccgaacac cgcctggccg atgcctgggc
     1861 agaattgctt aagcatcctg ccaatgccac cgtggtgcac cccaacgcct accggctggc
     1921 gtttgccgac cctgagttca tgttgcgccg cgacacgcgt ggcattcgca tccagcttga
     1981 gatgctcaag cccgacctcg accaggccga acaacgcatc gacaacaccg tggtggtgtt
     2041 tggcagtgcc cgcttttgct cgcccgagac cgcccagcag gcgatggcac aagcccagtt
     2101 cagcggcgac gcgcaggcca ttgcgctggc gcagcggcaa ctgcgcaatg cccaccatta
     2161 cgatcaggcg cgcctgtttg ctcggctggt ggctggctac agcgctgcac ttgatgtcaa
     2221 tgaacgcctg tacatctgta ccggcggtgg ccctggcatc atggaagcgg ccaaccgggg
     2281 tgcgcatgaa atgggtgcgc caacggtggg gttgaacatc accttgccgc atgaacagca
     2341 cgccaacccc tatgtcacac cgtccctgag cttcaagttc cattactttg cgctgcgcaa
     2401 gatgcatttc atgatgcggg ccaaggcgct ggtggctttt ccgggcggtt ttggcacgct
     2461 ggacgaactg tttgaagtga tcacactggt gcaaacccgc aaatcgcaac ccgtgccgat
     2521 cgtgctgttt ggcagcgact attggaggcg cctgctcaac accgaggtgc tgctggaaga
     2581 aggcgtggtg tcaagcgaag acctggagct gttccactac gtggacgagc cgcaagctgc
     2641 ctggggcatc atcaaaggtt tttacggctt gtgatgcgcc gccctgggtg gccgcaaacg
     2701 ccaggcaacg ccagaccctg ctgcgacacc
//
LOCUS       DAOSLL010000634         2726 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_32380_length_2726_cov_3.548484, whole genome shotgun sequence.
ACCESSION   DAOSLL010000634 DAOSLL010000000
VERSION     DAOSLL010000634.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32380_length_2726_cov_3.548484"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="PLB25_20145"
     CDS             complement(<1..664)
                     /locus_tag="PLB25_20145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOE43917.1"
                     /translation="MSMLAWGALIEGMLESVWLVDPVSLRILAVNQAASQLLGLTSQD
                     MVNKPVIELTATPEDQFFWEDVAAGAVDGIHSETWLRAADGIAVAVDRRVSRVWSADH
                     QAVYVVGIRDLRQQRRVEEELERLVAELRATLESTADGILVSDMNGDIRNYNRHFAEL
                     WEVPEALLLKRNDRALYAHMVDCVVDSNGYQARLLEIADQPLLEACDVLMLRSGRVLE
                     RVT"
     gene            complement(661..1953)
                     /locus_tag="PLB25_20150"
     CDS             complement(661..1953)
                     /locus_tag="PLB25_20150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007858553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3391 domain-containing protein"
                     /protein_id="HOE43918.1"
                     /translation="MNDDKSALIDPRQLQIGMYVELELGWMAHPFPKGSFRISSEKQI
                     ETIRGLGLPSVRYVPAKSDLPTIESTPGSDPVAPPVTSAVDDPAASQAKQLGRDRSEL
                     LGAQHRDLIACERRFGAAAREYKLVLEKINTNPTAATAQCLGLVNGFVGEMLGEGESA
                     IRLLSEGMGDKASMHPVNVSIVALLLGKYLGLSEADMADLGVAAFLHDIGKFQLPDRV
                     RWLEDNFTANEYKLYQEHVAQGVLLGKRMEVSAGTLQAIAQHHELVDGSGYPARVTGD
                     RMSLPAKILSLVNRYDNLCNPSRPSASITPHEALSLIFAQLKSRFDPVVLGGFVRMMG
                     VYPPGSVVQLTDERYALVVSVNSSRPLKPRVIVYDTRVPKNEALILDLEQNPALGIRR
                     SLKPSGLPRAAMDYLSPRQRICYFFERSVDKVPVEVSP"
BASE COUNT          608 a          772 c          733 g          613 t
ORIGIN      
        1 gtgtgacgcg ctcgagcacg cgccccgagc gcagcatcaa cacatcgcag gcctccagca
       61 agggttggtc ggcaatctcc agcagcctgg cctggtagcc gttgctgtca accacgcagt
      121 caaccatatg ggcatacaaa gcccggtcat tgcgctttag cagcaaggcc tcgggcacct
      181 cccacagttc ggcaaagtgc cggttgtagt tgcgaatatc gccattcatg tcactgacca
      241 gaatgccgtc agcggtggac tccagcgtgg cgcgcagttc agcgaccagt ctttccagtt
      301 cttcttcgac acgacgttgc tggcgcaagt cccgaatgcc caccacgtac accgcctgat
      361 ggtccgcaga ccaaacccgg gaaacacggc gatctaccgc cacagcaatg ccgtcggcag
      421 cgcgcaacca ggtttcagaa tggataccat cgacagcgcc agcggctacg tcttcccaga
      481 aaaactggtc ctcgggcgtg gccgtcaact cgattaccgg tttgttcacc atgtcctgtg
      541 aggtcaaccc gagaagttgg ctggcggcct gattgacggc aagaatgcgt aggctgaccg
      601 gatcaaccag ccagactgat tcaagcatgc cttcaatcag cgcgccccag gcaagcatgc
      661 tcatggcgac acctcaaccg ggaccttatc aacggaacgt tcaaaaaaat agcaaatccg
      721 ctgacggggt gacaggtaat ccatcgccgc acggggcaaa ccggacggct tgagactgcg
      781 cctgatacca agtgccgggt tttgctccag atccagaatc agggcttcgt tcttgggcac
      841 cctggtatcg taaacaatca ccctgggctt gagtggtctc gatgagttga ctgataccac
      901 cagggcgtag cgctcatcgg tgagctgaac cactgagccc ggcggataga cccccatcat
      961 ccggacaaag ccgcccaaca caaccgggtc aaaacgtgat ttgagttgcg caaaaatgag
     1021 cgacaacgct tcgtgcggcg taattgatgc gctgggacgg cttgggttgc acaagttgtc
     1081 atagcggttg accaaagaca ggattttggc tggcagcgac atgcggtcac cggtgacgcg
     1141 agccgggtag ccactgccgt caaccaattc atggtgctgg gcaatggcct gcagggtccc
     1201 cgcagaaacc tccatgcgtt tgcccagcag tacgccttgc gccacatgct cctggtacag
     1261 cttgtactca tttgcggtga aattgtcttc cagccagcgc acccggtcag gcaactggaa
     1321 tttgccgatg tcatgcaaga acgcagcaac tccgaggtcg gccatgtcag cctcggacaa
     1381 gcccagatat ttgcccaaca acagtgcgac gatgctgaca ttcaccgggt gcattgatgc
     1441 cttgtcgccc attccttcgg atagcagacg aatggctgac tcgccctctc cgagcatttc
     1501 gccaacaaag ccgttgacca gccccaggca ctgtgccgtg gccgccgtcg ggttggtgtt
     1561 gattttttcg agcaccaact tgtactcacg tgctgcggca ccaaaccgac gttcacaggc
     1621 tatcaagtca cggtgttgcg cacccagcaa ttcactgcga tctctgccca attgctttgc
     1681 ttgcgacgct gctggatcgt caacagcgct tgtcaccggt ggggctaccg ggtcagaacc
     1741 cggtgtcgat tcgatcgtgg gcaagtcgct tttggccgga acatagcgaa cgcttggcaa
     1801 gccaagacct cgaatggttt caatctgctt ttccgatgaa atcctgaagc tgcctttcgg
     1861 gaacggatgc gccatccagc caagctcaag ctcaacgtac atgccgattt gcaattgcct
     1921 gggatctatc aacgctgact tgtcgtcgtt catttgccat tcatgatcaa aattgttgag
     1981 ccagaggcca agtagttcgc ctggcagcga ttgctctccc atcggaggag tgcgagaagg
     2041 tcttgtgcca atgcgtaatc ttagcctcat ggtgcaggca tttttactac aacaatgcat
     2101 gttggtgtcg attggcaatt taatgtccat cacaaggtta aatccaccgg cttccagccg
     2161 gtcagcttca gctatgagac tttttgagcc gggcagtgaa cgtgaagact tgttttctaa
     2221 gcgagtcaag ttgtcatcga cgagttttct gtgccttgtc tttgcagtga aatcatctgg
     2281 tgtctgaagt tgtatgggtg tctattcgaa tggtcaaatg cgagacgaga cggtgtcaag
     2341 acgcgtcgtt taggcgacgc gtatcaggac ttccagtccg taacccaaac ccaccagctt
     2401 cagctcgtgg ttgtttagtt tgagagctgg caggttgtgc catatcttcc caacctgatg
     2461 ctgatgctca ttcgtcacca ggcattgaac gcaagtggcg atggcgcacc tgtatctgcg
     2521 tctttaccgc gttctcgctg gtgctcatca acttcatgga tgcgcagatg cggcttgatc
     2581 catttgaaga gccgcaataa gcgaccctga aattgaaatc gacaccaatc cgtttcagcc
     2641 gcaaaccggc atggctattg ggtttgagtc aaacttcaag cacctttgcc agacggtaaa
     2701 ggtcggcaag gtattgggat gtggtc
//
LOCUS       DAOSLL010000635         2725 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_32389_length_2725_cov_5.034831, whole genome shotgun sequence.
ACCESSION   DAOSLL010000635 DAOSLL010000000
VERSION     DAOSLL010000635.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32389_length_2725_cov_5.034831"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(400..1026)
                     /locus_tag="PLB25_20155"
     CDS             complement(400..1026)
                     /locus_tag="PLB25_20155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HOE43919.1"
                     /translation="MNKIDLKQQMKHLYHGSARSVVQVDVPALNFLMVDGIGDPNTSP
                     AYAQAVEVLFAVSYAAKFLVKKGPQALDYAVMPLEGLWWADDMAVFAGGDKSQWQWTM
                     MIMQPTFVSRETIHQAMADAQKKKNLPALDRLRLEAFHEGACAQLLHVGPFSEEGPSI
                     ERLHAFIDARSGRTGRHHEIYLSDIRKAAPEKWKTIIRQPMRAVADAS"
     gene            complement(1397..2242)
                     /gene="queF"
                     /locus_tag="PLB25_20160"
     CDS             complement(1397..2242)
                     /gene="queF"
                     /locus_tag="PLB25_20160"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005800674.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HOE43920.1"
                     /translation="MIPSDPIPPDRPTIFTDHCDATLLFPICRATRRELLGLGSPLPF
                     LGADLWTAFELSWLNARGKPQVALAHITVPCESVNLIESKSLKLYLGSFNNARFADAN
                     EVCSRLRTDLTEAAWRGGVVQSSVGVKLIAADLFDREPLYELDGLSLDRLDLDCDRYT
                     PAPDLLSSATDEAPVSEVLTSNLLCSNCPLTGQPDWGSLQISYFGPQIEQSGLLRYIV
                     SFRHAGGFSEDCVERIYMDIWQRCQPHKLVVHARYTRRGGLDINPFRTSYPQALPRNS
                     RTARQ"
     gene            complement(2478..>2725)
                     /gene="yaaA"
                     /locus_tag="PLB25_20165"
     CDS             complement(2478..>2725)
                     /gene="yaaA"
                     /locus_tag="PLB25_20165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HOE43921.1"
                     /translation="AVDLAVLKARVINCVFEDFKGGKYQIISFHAKRARGLMARFAAT
                     QRVVLPEQLKAFAAEGYAFDSGASGPARLVFRRSLAQ"
BASE COUNT          604 a          789 c          760 g          572 t
ORIGIN      
        1 gtggcgctgt tgttttcgat cacacgcgtc tggaacaagc ccgtcgtgct ggtggtcgat
       61 ggcaattggg cacaccacat tgagctaaag gggccaggtt cgaattgttt gcggccatgg
      121 gtgcatgagt tggaaaacat aggttcaccg aattgggctt tgaccccaat tctgcgggcc
      181 aaaccagcca cggtgtcacc aggtgccccg ctgcggcaag cacctgccca atcagggctt
      241 accgtgatat tgccttgtaa ccattgtcct tcaaacgctg aaagctatca aacagggagc
      301 agttcggggc caaccgacac cagataagcc gcagataagc cgagtctcag atcaaacgag
      361 cctggcccct ctaataggtg ttatcgggca aatttgtcgt caagacgcgt cggccaccgc
      421 gcgcatcggt tgccggatga tggttttcca tttttccggg gccgccttgc gaatatcgct
      481 caaataaatc tcgtggtgtc ggccggtgcg gcccgatctt gcgtcgatga acgcatgcag
      541 gcgctcaatg cttggaccct cttcggaaaa tggtcccaca tgcaagagtt gggcacacgc
      601 gccttcatga aacgcttcca gccgcagccg gtcaagggca ggcaggttct tctttttctg
      661 cgcatcagcc atcgcctggt gaatcgtctc gcggctgaca aaggtcggct gcatgatcat
      721 catcgtccat tgccactgtg acttgtcgcc gcccgcgaag acggccatgt cgtcggccca
      781 ccaaagccct tcgagtggca tgacggcata gtccagtgcc tgaggcccct ttttgaccaa
      841 aaacttcgcc gcataggaca ccgcaaacag gacttcaacg gcttgcgcgt acgcgggtga
      901 ggtgttgggg tcgccaatgc catccaccat caggaagttg agcgcaggta cgtcaacctg
      961 caccaccgaa cgggcgctgc catggtacag gtgcttcatt tgctgcttga ggtcaatttt
     1021 gttcatagca tttccttcgg ctaaggtttc ggcattcaga atgcgctgta agtagatgat
     1081 tcaataggat aaaaagccca ttagctggat aagttggtgg ttttgaacag gcccaggaaa
     1141 caagcctgaa ggccactttg aatcatgaca caactgcctg cgcgcctcca gccaagacct
     1201 cagactttct tcacaatggc gaccgtctgt ttgacaagct cgttgccgac ttatggaaag
     1261 aaatcggtct gcaggcactg ctttagcgct gtggcagagg cagatgactg ctgtcagggc
     1321 aaaacgagcg cttcaactat ccaggttgaa tccacttgaa tccggcagcg cgcaagccag
     1381 ccgacacccc tgttatttac tgccgtgccg tgcgtgaatt gcgcggcaat gcctgcgggt
     1441 aactggtgcg aaacgggttg atgtcaagtc caccccggcg cgtgtagcgg gcatgcacca
     1501 ccagcttgtg cggctggcaa cgctgccaga tatccatgta gatgcgttcc acacaatcct
     1561 cactgaaacc gccggcatgc cgaaagctca cgatgtagcg cagcaaaccg ctctgctcga
     1621 tctgcggccc aaaatagctg atttgcaagc taccccaatc gggctggccg gtcagcgggc
     1681 agttgctgca caacaagttg ctggtcagca cctcggacac cggcgcttca tcggtggccg
     1741 agctcagcaa atcgggtgcc ggggtgtagc ggtcgcaatc cagatcgagc cgatccaggc
     1801 tcaggccgtc gagttcatac aggggctcgc ggtcaaacag gtcagccgct atcagcttta
     1861 cccccacgct gctttgcacc acgccgccac gccaggcggc ttcggtcagg tcggtgcgca
     1921 aacgcgagca cacctcgttg gcatcggcaa accgggcgtt gttgaaactg cccaggtaca
     1981 gcttgagcga cttgctctcg atcaggttga ccgattcaca cggcacggta atgtgtgcca
     2041 gcgccacctg cggcttgccc cgcgcattga gccagctcaa ctcaaaagcg gtccacaaat
     2101 cggcccccag aaatggcaaa ggactgccta gcccgagtag ctcacgccgg gttgcgcgac
     2161 aaatcggaaa cagcagcgtg gcatcgcagt ggtcggtaaa aattgttggc cgatctggcg
     2221 gtattgggtc tgatgggatc atgtctgctc tccaaataat agctattggc gcaggataca
     2281 tatgcgctag aggccgattt gccctaagcc ggacaagccg gaatcgatga gccttcgtcg
     2341 cgagggcgcg actcctacac aaacagtcgg cgccgatttg aatttgtagg agtcgcgccc
     2401 tcgcggcgaa tgacttggaa tgtttgaatt cttcgcatga tatgcaaaga cttgacttgt
     2461 tagagactaa aaacagttca ctgcgccaga ctgcggcgaa agaccagccg cgccggcccg
     2521 gatgcaccgc tgtcaaacgc gtaaccctca gccgcaaagg ctttgagctg ctctggcaac
     2581 accacgcgct gggttgcggc aaaacgcgcc atcaatccgc gtgcccgctt ggcatgaaag
     2641 ctgatgatct ggtacttgcc gcccttgaag tcttcaaata cgcagttgat gacgcgggct
     2701 tttagcaccg ccaaatccac cgcct
//
LOCUS       DAOSLL010000636         2720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_32461_length_2720_cov_3.799625, whole genome shotgun sequence.
ACCESSION   DAOSLL010000636 DAOSLL010000000
VERSION     DAOSLL010000636.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32461_length_2720_cov_3.799625"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1445
                     /locus_tag="PLB25_20170"
     CDS             <1..1445
                     /locus_tag="PLB25_20170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007850411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HOE43922.1"
                     /translation="AANTRAVIPPPVTMALLPISEGVNALDFAQRLATHLKRLGTVCI
                     VDASSVDAALGQPGLARSASTDTDGNRQIALHLDQLELSHDFVLLVGDADASAWTQRC
                     SRYNDEMLLLAMASCPPAIHVTEQRYLMQRSERNDSAQILILLHAADTACPGGTRHWL
                     ERRPISDHVHVRPALDRDMARLARIQSRTAIGLVLAGGGAKGLAHMGLFRALQEHRIE
                     IDFVGGTSIGAIMAAMAASDQPLDALMPIARTAFGDKPTGDFNLLPLISLIRGQRLRR
                     ILQVALKHIFGKLPDIEDLWKNYFCVASNYTQAREQVISHGALSKSILASVAIPGAFP
                     PVLIDGDLLCDGGTFNNFPVNVMRRRRGVGKVIGMELGTRKPLPVALEEVPGTWALLR
                     DRLRPRPKRRYTLPTLMTYLLNVSIMYSTSRQRESQKLTDLYFKPALARVGMLQWGRF
                     DSIAEQGYVHACEVLDAMTDEQLRPFRPHA"
     gene            1508..>2720
                     /locus_tag="PLB25_20175"
     CDS             1508..>2720
                     /locus_tag="PLB25_20175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43923.1"
                     /translation="MATGPIHHIIDLFNEAFRGLSVEVSAVDVEKLANLVQHAMTGEN
                     RTFHNTSHALGMCEGMKPLQVLAALFHDVVYCQIDGGLPALLEPFLEGVTSAEDDRLI
                     LQKIAPADRATTICADVFGFRPGQVLPLQVGMNEFLSAVVAVRVLQRHLRLAHLIAIA
                     ASIEMTIPFRTTDADGHTAAQALARRVRRISETWHTELSLSQQDIAAFVKTVVTDAVL
                     LANRDTSGFTEAGPRQCLTNTWLLLKEANASLATHGVHSLVAYREALMRMDVFFSRLD
                     PVLICQTYDDQPGTDTLRKMGVNAGRNVQFSRDYLAVEIAFVAILEALALCTGSDCPA
                     AILLGDMAVALPDWALGTPGDPALLDLLDQEHLTASINDLTASPLKPLMYRFLGHAGT
                     EHAASLARQMFA"
BASE COUNT          518 a          858 c          810 g          534 t
ORIGIN      
        1 gcgcagccaa cacccgtgcg gtgattccgc cgccagtcac catggcgctg ttgccgatca
       61 gcgagggagt caacgcgctg gattttgcac agcgcctggc aacccacctg aagcgactgg
      121 gaaccgtctg cattgtcgat gccagcagcg tcgatgccgc cctcgggcag cccggcctgg
      181 cccgcagcgc aagcaccgac accgacggca accggcaaat tgcgctgcac cttgaccaac
      241 tggaactgtc gcatgatttt gtgctgctgg tcggtgatgc cgatgccagt gcctggaccc
      301 agcgctgcag ccgctacaac gatgagatgc tgctgctggc catggcaagc tgcccccctg
      361 cgattcatgt gaccgagcaa cgctacctga tgcagcgcag cgagcgaaac gactcagccc
      421 agatattgat cttgcttcac gccgctgaca cagcctgccc tggcggcacc cggcactggc
      481 ttgaacgccg cccgatctcc gaccatgtgc acgtcaggcc agcgcttgat cgggacatgg
      541 cacggctggc gcgcatccag agccgcactg ccatcggtct ggtgctggcc ggtggtggtg
      601 ccaaggggct ggcccacatg ggtttgttcc gggccctgca ggaacaccgc atcgaaatcg
      661 actttgtcgg gggcaccagc atcggtgcca tcatggcggc gatggccgcg tcagaccagc
      721 cattggatgc cttgatgccc attgcgcgca ccgcctttgg cgacaagcca accggagact
      781 tcaacctgct gccgctgatt tccctgatac gcgggcagcg cctgcggcgt attttgcagg
      841 tggcgctgaa gcacattttt ggcaaattgc ccgacatcga agacctttgg aaaaactact
      901 tttgcgtggc cagcaattac acccaggcgc gcgaacaggt catcagccac ggcgcgttga
      961 gcaagtccat cctggccagc gtcgccatac cgggggcctt tccaccggtg ctgattgacg
     1021 gcgacctgct ctgcgacggt ggcaccttca acaactttcc ggtcaatgtg atgcgcaggc
     1081 ggcgcggggt tggcaaagtc atcgggatgg aactgggcac ccgcaagccg ctgcccgtgg
     1141 cgctggaaga agtaccgggc acctgggcac tgctgcgcga ccggttgcga ccccgaccca
     1201 agcgccgcta cacactcccc acgctgatga cctatttgct caatgtcagc atcatgtaca
     1261 gcacctcgcg ccagcgcgag tcgcaaaagc tgacagattt gtacttcaag ccagcgctgg
     1321 ctcgtgtcgg catgctgcaa tggggccgct ttgacagcat tgccgagcaa ggctatgtgc
     1381 acgcctgcga ggtgctcgat gccatgaccg acgaacaatt gcgaccgttt cgcccgcatg
     1441 cctgacaggc tgacctgtgg caccggtctg gggcaaaatc cagacttggg aaaaggagca
     1501 atttagcgtg gcaaccggac caatccatca catcattgac ctgttcaacg aggcttttcg
     1561 gggcttgtca gttgaggtct cagcggttga tgtggaaaag ctggccaatc tggttcagca
     1621 cgccatgact ggcgaaaaca gaaccttcca caacacctcg cacgccctgg gcatgtgcga
     1681 aggcatgaaa cccctgcaag tactggctgc actttttcat gatgtggttt attgccagat
     1741 tgacggtggc ttgccagcgc tgctcgaacc ctttttggaa ggcgtgacca gcgccgagga
     1801 tgaccgcctg atcctgcaga aaattgcccc cgccgaccgc gccaccacga tctgcgccga
     1861 tgttttcggt tttcgtcctg gccaggtgct gccgctgcag gtcggcatga acgaattttt
     1921 gagcgctgtc gtggcggttc gggtgctgca gcgccatctg cgcctggcgc acctgattgc
     1981 catcgctgcc agcatcgaaa tgacgattcc gtttcgcaca acggacgcag acggtcacac
     2041 tgcggcgcag gcactggcgc gaagagtgcg ccgaatcagc gaaacctggc acaccgagtt
     2101 gtcgctgtcg cagcaggaca ttgcagcctt tgtcaaaacg gtcgttaccg atgcggtgct
     2161 gctcgccaac cgggacacct ccgggtttac cgaagctgga cccagacagt gcctgaccaa
     2221 cacctggctg cttctcaagg aggctaacgc ctcgttggca acccatggcg tgcattcatt
     2281 ggtggcctac cgcgaggcgc tgatgcgaat ggacgttttt ttcagtcggc ttgacccggt
     2341 gctgatctgc cagacctatg acgatcaacc cggcaccgac acgctgcgca agatgggtgt
     2401 caatgccggg cgcaacgtgc agttttcgcg cgactacctg gccgtggaaa ttgcctttgt
     2461 cgcgatcctt gaggccctgg ccctgtgtac cggctccgat tgccctgcgg ccatactgct
     2521 cggtgacatg gcggttgcgc tgccggactg ggccttgggc acgcccggcg accctgcatt
     2581 gctcgatttg ctggaccagg agcacctgac ggcatccatc aatgacctga cggcttcgcc
     2641 attgaagcca ctcatgtacc ggtttctggg gcatgcgggc accgagcatg ccgccagcct
     2701 ggccaggcaa atgttcgccg
//
LOCUS       DAOSLL010000637         2713 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_32564_length_2713_cov_3.345372, whole genome shotgun sequence.
ACCESSION   DAOSLL010000637 DAOSLL010000000
VERSION     DAOSLL010000637.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32564_length_2713_cov_3.345372"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="PLB25_20180"
     CDS             complement(<1..313)
                     /locus_tag="PLB25_20180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HOE43924.1"
                     /translation="MKKHIYIYSPSGAVRDQAAFRRGVTRLQALGHEVEVDEAALVCF
                     QRFAGDDETRLAAVHRATASGADVALISRGGYGLTRILPGIRYKRVRKAIDRGMLFVG
                     LS"
     gene            complement(313..1395)
                     /gene="tadA"
                     /locus_tag="PLB25_20185"
     CDS             complement(313..1395)
                     /gene="tadA"
                     /locus_tag="PLB25_20185"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HOE43925.1"
                     /translation="MTPTAEAAQDLHWMRLALAEARAAGEAGEMPVGAVVVCRGNIVA
                     TGRNAPISTHDPTAHAEIVALRAAARQLGNYRLTDCDLFVTLEPCSMCAGAILQARVK
                     RVVFGAANDKGGAAGSVIDLFAHRQLNHQTKIQGGVLADACAEGLQGFFREQRVSKRL
                     ERAVARSALRDDALRTPESRFAALPDWPTASCYVSDLPYLAGLRLHYLETGSVNASQT
                     LVYLHGPQDWCYAWRSSIIRGQSEGVRLVCPDLIGFGKSDKPKKASFHRLQWHAQVLL
                     QWMAQLRLSNLTLIAPVQMHELVQLVVAGSAPQVIRTRYSQPDMLDAAALAAPFPDIG
                     HRAALRAFATMLTHTVSPTMPSTPLF"
     gene            complement(1392..1637)
                     /locus_tag="PLB25_20190"
     CDS             complement(1392..1637)
                     /locus_tag="PLB25_20190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43926.1"
                     /translation="MRGMLTLAGLLVALGVASVLVRKQMVAMRTPVPSLQLPAKAATG
                     NAASASDSALQQSQQMQQQYKQAIEGAIQARQMPDDK"
     gene            1890..>2713
                     /locus_tag="PLB25_20195"
     CDS             1890..>2713
                     /locus_tag="PLB25_20195"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HOE43927.1"
                     /translation="MLLLTDLYRTKWLDAGADAILVWRIAALVFLLSYRFAVKKLTPK
                     LLRLRYFLIVGMFLCCWACAVLADITGDMSTFAVGALGMAAACPLPGRFNSSLFVISG
                     AALIGWLLFHYPNAGLFWVSSIIPTCVIGIVIEKFTFTAALREFSHRKGIERQRERAD
                     VLLYNVFPQSVAASLKAGQRSVALHSEVTILFADVVGFTQLASRLLPSQLVGLLEDIF
                     GRFDQLAQKHGVEKIKTIGDAYMAISGAPIAVDHQVERMADFALELVQVCKQISVN"
BASE COUNT          541 a          800 c          750 g          622 t
ORIGIN      
        1 cgctgagacc gacaaacagc atgccccggt cgatggcttt gcggaccctt ttatagcgaa
       61 tacccggcag gatgcgcgtc agcccgtagc cgccgcgcga tatcagagcc acgtcggcac
      121 cactggcggt tgcgcggtgg acggcagcca ggcgggtttc gtcatcgcca gcaaaacgct
      181 gaaagcagac caaggcagct tcatcaacct ctacctcatg tccaagcgcc tgcaggcgag
      241 tcacgccacg ccggaaagcg gcttggtcgc gtaccgcgcc cgatggcgag taaatgtaga
      301 tgtgcttttt catcaaaata gtggtgttga tggcatcgtt ggtgacaccg tgtgcgtcag
      361 catggtagca aacgcccgca aagcagctcg atggccgata tcaggaaaag gtgctgccag
      421 tgcagctgca tccagcatat ccggttgact gtaacgcgtc ctgatgacct gtggcgcgga
      481 tccggccacc acaagttgta ccaattcatg catttgaacc ggagcgatca gtgtcaagtt
      541 ggacaaacga agctgtgcca tccattgcag cagcacctgc gcgtgccatt gcagcctgtg
      601 aaaagatgcc tttttgggct tgtcactttt tccaaagcca atcaggtcgg ggcaaaccaa
      661 gcgaacaccc tcgctctgcc cccggatgat gctgcttctc catgcgtaac accagtcctg
      721 cggaccatgc agatagacca gggtttgact ggcattcact gacccagtct ccaagtaatg
      781 caaccgcagt cccgcaaggt aaggcaggtc gctcacgtaa cacgatgccg tcggccaatc
      841 cggcagcgcg gcaaaacgac tctcgggagt gcgcaaggca tcgtcgcgca gcgcactgcg
      901 tgcaacggcc ctttccaacc gcttgctgac gcgctgttcc ctgaaaaagc cttgcaaacc
      961 ttcggcgcaa gcatcggcta ggacaccgcc ttgaatcttg gtttggtggt tgagctgcct
     1021 atgggcaaac agatcaatca ctgaccccgc cgcgccgccc ttatcgttgg ctgcaccaaa
     1081 taccacccgt ttaacccttg cctgaagtat tgccccggcg cacatgctgc aaggctccag
     1141 cgtcacaaac agatcacagt ctgtcagtcg gtaattgcca agttgccgcg ctgcagcgcg
     1201 caaggcgacg atttcggcgt gcgcggtcgg gtcgtgggtg gagatcgggg cattgcgtcc
     1261 ggtcgcaacg atgttacccc gacacaccac cacagctccc accggcattt cgccagcctc
     1321 cccggccgct cttgcctcgg ccagcgccag gcgcatccag tgcaggtctt gcgctgcttc
     1381 agccgtaggt gtcatttgtc gtcaggcatc tggcgtgcct gaatcgcacc ttcaatcgcc
     1441 tgcttgtatt gctgctgcat ttgctggctt tgctggaggg cgctgtctga tgccgacgcg
     1501 gcgttgccag tggctgcttt ggctggcagt tgcagactgg gcaccggtgt ccgcattgct
     1561 accatctgct tgcgcaccag cacactcgcc acacccagcg ccaccaacag ccccgccaaa
     1621 gtcaacatgc cgcgcatcga atgcctcctt gtcaaggatc atgccggtgg cacgcccgga
     1681 gtttccccgg ggcctctcca aacatcgaaa agatatactg atcaggacat aacgcaccga
     1741 gccgcacatt ggtcagtttc tcgaatcatc tgcccgcacc aagtcctgat tttctcacgt
     1801 cttggattga ggaattcagc caggaattgg tcgagtctgc gctgcggcgg gccagtgtcg
     1861 tcacaaacac actcatcatc atcgaaggtg tgctgctgct gaccgatctt tatcgcacca
     1921 agtggcttga tgcgggggcg gatgccattc ttgtctggcg tatcgcagca ctggtgttcc
     1981 ttctgagcta tcgttttgcc gtcaaaaaac tgacaccgaa gcttctgcgg ctgcggtatt
     2041 ttctgattgt tgggatgttt ttgtgttgct gggcctgtgc tgttctggcg gacattaccg
     2101 gcgacatgtc aacctttgcc gtcggggcct tgggcatggc agcagcatgc cccttgccgg
     2161 gacggtttaa ctcatccctg ttcgtcatat cgggggcggc cctgattggc tggctattgt
     2221 tccactatcc caatgcaggt cttttttggg tcagcagcat catccccacc tgtgtcattg
     2281 gcatcgtgat tgagaagttt accttcactg cggctttgcg agagttttcc catcgcaagg
     2341 ggattgagcg gcaacgcgag cgcgccgatg tgcttttgta caacgtgttt ccccaatcgg
     2401 tagcggcatc gctcaaggca ggccagcgct ctgttgcttt gcacagcgaa gtgactattt
     2461 tatttgccga tgtagtcggt tttacccagt tggccagccg cctgttgccc tctcaattgg
     2521 tcggtttgct tgaagatatt tttggaaggt ttgaccagct cgcgcaaaaa catggtgtgg
     2581 agaaaatcaa gaccatcggt gatgcctata tggccatttc cggtgcaccg atagcggttg
     2641 accatcaggt agaacgcatg gcggattttg cgctggaact ggtccaggtc tgcaaacaga
     2701 tttccgtcaa cag
//
LOCUS       DAOSLL010000638         2702 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_32721_length_2702_cov_4.046845, whole genome shotgun sequence.
ACCESSION   DAOSLL010000638 DAOSLL010000000
VERSION     DAOSLL010000638.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32721_length_2702_cov_4.046845"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="PLB25_20200"
     CDS             complement(<1..184)
                     /locus_tag="PLB25_20200"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013804765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HOE43928.1"
                     /translation="MVPHLITALTGPINELEQRVLDSMPTIERWFRLEWMEHTPPFYS
                     SVDIRNAGFKLAPVKTN"
     gene            complement(317..1684)
                     /locus_tag="PLB25_20205"
     CDS             complement(317..1684)
                     /locus_tag="PLB25_20205"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004340442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HOE43929.1"
                     /translation="MNAFNITDYAESLGLQAKQASALMAKAQAATRNIALRKLAELLR
                     TNLGELQIENAKDLERAITAGLPAPMVDRLKLAPKVIETCAQGCEQLAAMADVIGEVT
                     TLRQQPSGIRVGQMRVPIGVFGMIFESRPNVTIEAAGLSIKSGNACILRGGSEAIDSN
                     KALARLVQQALHLSALPETAVQLVQTTDRAVVDLLIAMPRYVDVIIPRGGKGLIERVS
                     AGAKVPVIKHLDGNCHTYVDDPCDLDMAVRVADNAKTSKYSPCNATESLLVARGVAHA
                     FLPQIASIYAAKGVEMRCDAEALQVLQTFEHNRALAYAGNALTASYSVAEPSLGNAGS
                     AAFKLVNAQESDWFEEYLAPIISIKVVAGVDEAIGHINHYSSHHTDAILTRDHLHAQR
                     FLREVDSASVMVNTSTRFADGFEYGLGAEIGISTDKFHARGPVGIEGLTSLKYVVLGD
                     GEVRG"
     gene            1762..2628
                     /locus_tag="PLB25_20210"
     CDS             1762..2628
                     /locus_tag="PLB25_20210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007583579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HOE43930.1"
                     /translation="MNTITDLSPSWQAWLDDNLARGCSPASMLDSMIKANFDAAAASV
                     ALQQAQARRQGASHQPEPQAPAGGYVRETPRLPQSGNVIRTHDRDIRVTLRLHQPTLA
                     LFDNLMSAEECDELISLARIKLKRSATVDPVTGHEQVIDARTSFGTFFRLNEDDFIAR
                     LDRRIADLMHWPIENGEGIQILHYPVGGEYRSHFDYFPPEEPGSLAHLAKGGQRVSTL
                     VMYLNDVEEGGETSFPSLKLSITPKKGAAAYFEYANSLGQVDPLSLHAGTPVVRGEKW
                     IATKWMRERRYG"
BASE COUNT          550 a          814 c          798 g          540 t
ORIGIN      
        1 gattggtttt caccggagcc aatttgaagc cagcattgcg aatatcgacc gagctgtaaa
       61 acggcggtgt gtgctccatc cattccaggc gaaaccagcg ctcaatggtt ggcatcgagt
      121 ccagcacgcg ctgctcaagt tcgttgattg gcccagtcag ggcggtgatg aggtggggaa
      181 ccatgggaaa cccttgcaaa gtggtaaccc gaattttagg gcgctcaatt gccaccccgg
      241 caaaaacggc acaaggtcgt gtccgggcag cgacagcttg caagcctgcc gccacccctg
      301 ggttgcccgg tgaaactcag ccgcgcactt cgccgtcgcc cagcaccaca tacttgagtg
      361 atgtcagccc ctcgatgccg accgggccac gggcatgaaa cttgtcggtc gagatgccga
      421 tttcagcccc cagcccgtac tcaaagccat cggcaaagcg ggtgctggtg ttgaccatga
      481 cgctggccga atcaacttct cgcaaaaatc gctgcgcatg caggtgatcg cgtgtcagga
      541 tggcatcggt gtggtggctg ctgtaatggt tgatatggcc gatggcttcg tccacaccgg
      601 ccaccacctt gatgctgatg atcggggcca ggtattcttc aaaccagtcg gattcttgcg
      661 cgttgaccag cttgaaagcc gccgaaccgg cgttgccaag acttggctct gctacagaat
      721 agctagctgt cagcgcattg ccagcatagg ccagagcccg attatgctca aatgtctgga
      781 gcacctgcaa ggcttcggca tcacaccgca tctcaacccc cttggcggcg taaatgctgg
      841 caatctgcgg cagaaatgcg tgcgccacgc cgcgcgccac cagcaggctc tcggtggcgt
      901 tgcacgggct gtatttgctg gttttggcgt tgtcagcgac ccgcaccgcc atgtcgagat
      961 cgcacgggtc atccacatag gtatggcaat tgccgtccag gtgcttgatg accggcactt
     1021 tggccccggc gctgacgcgc tcgatcagcc ccttgccgcc acgtggaatg atcacatcaa
     1081 catagcgcgg catggcaatc agcaagtcca ccacggcgcg gtcggtggtt tgcaccagtt
     1141 gcactgcggt ctcgggcaga gcgctcaggt gcagcgcctg ttgcaccagc ctggccaagg
     1201 ccttgttgga gtcgatggcc tcggagccac cgcgaagaat gcaggcattg ccgctcttga
     1261 tggacaggcc cgccgcctcg atcgtcacat tgggccgact ctcgaaaatc atgccaaaca
     1321 cgcctattgg cacacgcatc tgcccgaccc ggatgccgct gggctgctgg cgcaaggtgg
     1381 tcacctcacc aatcacatcg gccatggcgg ccagttgttc acagccctgg gcacaggttt
     1441 cgatcacttt gggagccaat ttgagccggt ctaccatcgg tgcgggcagc ccggcggtga
     1501 tcgcgcgctc aaggtccttg gcgttttcga tctgcaactc gcccagattg gttctgagca
     1561 attcggcgag ttttcgtaga gctatatttc tggtagcggc ctgcgcttta gccataaggg
     1621 ctgaagcctg ttttgcctgc aaacccaagg attcggcgta gtcggtgatg ttgaatgcat
     1681 tcatgctgcc attttggcat gcgcaaacgg cctgtcaaac gctgccatgt ggcggataaa
     1741 ctcaggtgcg agaggtagca attgaacacc atcaccgatt tatctccttc atggcaagcc
     1801 tggctggatg acaacctggc acgtggctgc agtccggctt caatgctcga cagcatgatc
     1861 aaggctaatt ttgatgcggc cgctgccagc gttgcgctgc agcaggcgca agcccgcagg
     1921 cagggagcaa gccaccaacc cgagccgcag gccccggctg gtggttatgt tcgcgaaaca
     1981 ccgcgcctgc cgcaaagcgg caatgtgatt cgcacccacg accgcgatat ccgggttacg
     2041 ctgcgcctgc accagccgac cctggcgctg tttgacaacc tgatgagcgc cgaggaatgc
     2101 gacgaactga tcagcctggc acgcatcaag ctcaagcgct cggcaacggt cgatccggtc
     2161 acagggcacg agcaggtgat tgatgcgcgc accagttttg gcacgttttt ccggttgaac
     2221 gaagacgact tcattgccag acttgatcgc cgcattgccg acctgatgca ctggccgatt
     2281 gaaaacggcg aaggcatcca gatcctgcac tacccggtgg gtggtgaata cagatcgcac
     2341 tttgactact tcccgcccga agagccaggt agcttggcgc atctggccaa gggcggtcaa
     2401 cgcgtcagca ccctggtgat gtacctcaac gatgtggaag aaggcggtga aaccagcttt
     2461 ccgtcgctca aactgtcaat caccccgaaa aaaggcgcgg ccgcctactt tgaatacgcc
     2521 aacagcctcg gtcaggtcga tccgctgtcg ctgcatgctg gcacgccggt ggtcaggggc
     2581 gaaaaatgga ttgccaccaa gtggatgcgc gagcgccgct acggctgagg tgcttgccgc
     2641 accgtgggcg gctgaccgac tgcggctgac cgactgcggc tgaccgactg cggctgaccg
     2701 ac
//
LOCUS       DAOSLL010000639         2688 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_32939_length_2688_cov_4.677554, whole genome shotgun sequence.
ACCESSION   DAOSLL010000639 DAOSLL010000000
VERSION     DAOSLL010000639.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32939_length_2688_cov_4.677554"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="PLB25_20215"
     CDS             <1..1059
                     /locus_tag="PLB25_20215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43931.1"
                     /translation="SVLLDFGSARRIIGDGIQALTTVLKPGYAPIEQYVDDGTMKQGP
                     WTDVYALGGVLYHPATGKAPLQAVSRLMTDPLQPVHALMGDRFSMVFSNAVSKAMAVR
                     LEDRLQNVSELREMLGWSGGQSARVVTLPAATYAPTKAERAEPTLRPDTQVVEQVAAA
                     DQPTAWIQPPAPATPTATPSDSGAAALDPPMRGERPANLPEADTSNHIKLALFAAGIC
                     IIIVVAYFLLRPAVPVGAPVAPTPVAPTPVRQPVSSEKPPTAPAGASVSNPPPLASAA
                     SAAVTQTGIIKLNIRPWGTVVVDGITRGISNETQLQQIELTVGPHTIEIAKPDAPKLV
                     KTVEVSAGSPLTISHTFR"
     gene            1089..2654
                     /locus_tag="PLB25_20220"
     CDS             1089..2654
                     /locus_tag="PLB25_20220"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HOE43932.1"
                     /translation="MNSPQSTAKTASSSLPVGMRLAEFEITGVIGEGGFGIVYSAHDL
                     SLQRMVAIKEYLPTAFAQRADDGSVQVKSEEYRKTFTAGLTSFVNEARMLAKFSHPGL
                     VEVFRFWEASGTAYMVMRYYRGVTLREALRSSRELITEQWLCETLDPILLALKELHNE
                     RCFHRDVAPDNILVLPNGKSVLMDFGAARRIISGMTQALTTVLKPGYAPIEQYYDDGS
                     MPQGAWTDIYAVGGLLYYMMVGKVPVQAISRMMSDPLKPVSEVALGAYSERLCSVVMK
                     CLAVLPEHRYQSIDELRGALGWVILPVMSTLPRQPEAAPTTASEFPETVLMTPASAER
                     IALAQAGIAPRSSRMTAMVADTDHPKSGPDATVKSGASQPSDSKIQASGDRTGPPAAK
                     SERAPLMAEDLPAKAVKKAPSVTSQHLPAPGSRLSARQWLGLAVFVLALAGAGVFVSG
                     VYKSDVSVSEMTDAVPNVKKSAEVALPTPVAAATSSVPASVPATGTASGTATPIETSV
                     PPALAATPAALCP"
BASE COUNT          566 a          770 c          782 g          570 t
ORIGIN      
        1 tctgtactgc tcgattttgg ctcggcgcgg cgcatcatcg gtgacggtat ccaggcgctg
       61 acgaccgtgc tcaagcctgg ttatgcgccc attgagcagt atgttgacga tggcacgatg
      121 aagcaggggc catggaccga cgtttatgca cttggcggcg ttttgtacca tcccgccact
      181 ggcaaggcac cgctgcaagc ggtgtcgcgg ctcatgacag atccgctgca gccagttcat
      241 gccttaatgg gtgacaggtt ttcgatggtg ttttccaatg cggtcagcaa agctatggcg
      301 gttcgtttgg aggatcgcct gcaaaatgtt tcggagttaa gagaaatgtt gggttggagt
      361 ggcgggcaat cggcgcgagt cgtcactttg ccggcagcga cctatgcgcc gaccaaggca
      421 gagcgtgccg agccaacctt acgccccgac acccaggtcg tcgagcaggt ggccgctgct
      481 gatcagccga ctgcctggat tcagccaccc gcacccgcga caccgactgc aaccccgagt
      541 gattcagggg cggctgcttt ggacccacca atgcgcggtg aaagaccggc caatctacca
      601 gaagcagaca catcaaatca catcaagttg gcgctttttg ccgctggcat ctgcatcatc
      661 atcgttgttg cctatttttt gctgcggcct gcagtaccgg ttggcgcacc ggtcgcccca
      721 acaccggtcg ccccaacacc ggtcaggcag ccggtttcat ctgaaaagcc gccaactgcg
      781 ccagccggtg ccagcgtgtc caatccgccg ccattggcat ccgccgcatc ggccgccgtg
      841 actcaaaccg gtatcatcaa attgaacatt cgaccttggg ggacggtggt ggttgacggc
      901 ataacgcggg gcatttccaa cgaaacacaa ttgcagcaaa ttgagttgac ggtagggccc
      961 cacacgattg aaattgccaa gccagacgcc ccgaaacttg taaaaacggt ggaagtcagt
     1021 gccggttcgc ctttgactat ttcgcacacc ttcagatgaa cctgcaacga gcttagccgc
     1081 ccgcacccat gaatagccct cagtccaccg ctaaaacagc ttccagttcg ctacccgtcg
     1141 gcatgcggct ggcggagttt gagatcactg gtgtcattgg cgagggaggg tttggcattg
     1201 tgtacagcgc gcatgactta agcctgcaga gaatggtggc gatcaaagag tatttgccga
     1261 cggcttttgc gcagcgcgcc gatgatggca gcgtgcaggt gaaatctgaa gagtatcgaa
     1321 agacattcac tgcaggtctg acgagttttg tcaatgaagc acggatgctg gcgaagtttt
     1381 cgcacccagg gttggttgag gtgtttcgtt tttgggaggc cagtggcacc gcttacatgg
     1441 tgatgcgcta ttaccgtggc gtgacgctgc gcgaggcctt gcgctcatca cgcgaactga
     1501 tcaccgagca gtggttgtgc gaaacccttg acccgatact tctggcgctg aaagaactgc
     1561 acaacgaaag atgttttcac cgggatgttg cgcccgacaa catcctggtg ttgcccaatg
     1621 gcaagtcggt gctgatggat tttggtgcgg cacgccgcat catcagtggc atgacccagg
     1681 cgttgacaac ggtgctcaag ccaggctacg cgcccatcga gcaatattac gacgatggat
     1741 cgatgcccca aggagcctgg accgacatct atgccgttgg cggtttgctg tattacatga
     1801 tggttggcaa ggtgccggtt caagccattt cgcgcatgat gagtgacccg ttgaagccag
     1861 tttcagaagt ggcactggga gcctattccg agcggctttg cagcgtcgtg atgaagtgtc
     1921 tggccgtgct acccgaacac cgctatcaaa gtattgatga gttgcgtggt gccttgggct
     1981 gggtgatatt gcccgtgatg tcaacgctgc cgcgccagcc agaagcagcg ccgacaacag
     2041 cttctgaatt tccggaaacc gtgttgatga cacctgcatc ggccgagcga attgctttgg
     2101 ctcaggcagg gattgcgccg cgatcgagtc gcatgacggc aatggttgca gatacagacc
     2161 atccgaagtc gggccccgat gccactgtga aatctggtgc cagccaacct tccgactcaa
     2221 aaattcaagc atctggtgat cgcaccggcc caccggcagc aaagtcagaa cgcgcgcctt
     2281 tgatggccga ggatctgccc gccaaagcag tcaagaaagc gccgtctgtc accagtcagc
     2341 atttgcccgc ccccggctca agactgtctg cccgacagtg gctgggcctg gctgtatttg
     2401 tgcttgcgtt ggcaggcgca ggcgttttcg tctccggcgt ttacaaatcg gatgtgtcag
     2461 tgagtgaaat gaccgacgct gtgcccaatg tgaagaaatc ggctgaagtc gccttgccca
     2521 cgccagtggc tgcagccaca tcatcggtcc cagcctcggt gccagccacg ggtacagcct
     2581 cgggaacagc caccccgatt gaaaccagcg tgccgccagc acttgcggca actccggcgg
     2641 cactgtgccc ttgagttcac catcaacaat gacattgccc catccccc
//
LOCUS       DAOSLL010000640         2687 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_32962_length_2687_cov_3.384878, whole genome shotgun sequence.
ACCESSION   DAOSLL010000640 DAOSLL010000000
VERSION     DAOSLL010000640.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32962_length_2687_cov_3.384878"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            389..1552
                     /locus_tag="PLB25_20225"
     CDS             389..1552
                     /locus_tag="PLB25_20225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HOE43933.1"
                     /translation="MIKTAPDQHQDIRDAVRALCAGFPAAYHRQTDAQRGYPEAFVDA
                     LTKAGWLAALIPQQYGGSGLSLTQASVIMEEINRSGGNSGACHGQMYNMGTLLRHGSS
                     AQKALYLPKIASGEWRLQSMGVTEPGSGSDTTRISTTAVKKGDRYLVNGQKVWISRVQ
                     HSDWMILLARTTALAEVRKKSEGMSIFMVDLAQAERNGMTVRPIANMVNHETNELFFE
                     NLEIPAENLIGQEGQGFKYILDGLNAERALIAAECIGDGYWFLDRVTSYVSERAVFGR
                     PIGQNQGVQFPIADAFIEVEAANLMRYKACELFDAGQPCGAQANMAKYLAAKASWEAA
                     NACIQFHGGFGFANDYDVERKFRETRLYQVAPISTNLIYAYIAEHVLGLPRSF"
     gene            1734..>2687
                     /locus_tag="PLB25_20230"
     CDS             1734..>2687
                     /locus_tag="PLB25_20230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007833113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HOE43934.1"
                     /translation="MTRPLDGMTVVSLEHAIAAPFCTRQLADLGARVIKVERPGEGDF
                     ARSYDSRVMGLASHFVWTNRSKESLTLDLKQPAALAVLAQLLGQADVLVQNLAPGAAA
                     RMGLSFAALTAAHPRLIVCDISEYGENGPYRDKKAYDLLIQSEVGFLSVTGTPEAPSK
                     AGISVADIAAGMYAYTSILSALLLRGKTGQGSQIDVSMLESLAEWMGFPLYYTHAGAA
                     PPPRTAAAHASIYPYGPFQVGDGGVVMLGLQNEREWQAFCAGVLEDAAIASDPRFDSS
                     ANRNQNRDALRALILAVFGRLTTAQALARLDQAQIANARMND"
BASE COUNT          528 a          819 c          829 g          511 t
ORIGIN      
        1 cgctcgacat cgagaaatcc acggctggcc ggttgatcga tcaggtcgaa gaacagcggc
       61 tggatcaaac gacggcccag tgccggcgac cagtgcctgt ggggtattca tctgaccggg
      121 caggcgcggc ctctgctggc cgaggtcgag caggtggtgc aggctgcccg caccgagatg
      181 ctgcacgggc tgtcacaaaa gcagcaacag cgtctgtcag agaggctgca atgcgtgaag
      241 tcgaatcggc ttggggcgct gcagcagcat cggtcatcgc ttcacgagca gtggttcaaa
      301 gatggcacaa gctgacttgg gtgtcgctgc ctgcaacggt cagcagcgtt tgtgccgcac
      361 cacgcttgcc ccaactgagg agtccagaat gatcaagacc gcccccgacc agcaccagga
      421 catccgcgat gcagtgcgtg ccctgtgcgc cgggtttccc gctgcctacc accgccagac
      481 cgacgcgcag cgtggctacc ccgaggcctt tgtcgatgcc ttgaccaaag ccggctggct
      541 ggccgcgctg attccccagc aatacggtgg ctctggcctg agcctgacgc aggcatcggt
      601 gatcatggaa gagatcaatc gcagcggtgg caactcgggg gcctgccatg gccagatgta
      661 caacatgggc accctgctgc gccacggctc gagcgcgcag aaggcgctgt acctgccaaa
      721 aatcgccagc ggcgaatggc gcttgcagtc gatgggcgtg accgaacccg gcagcggcag
      781 cgacaccacc aggatcagca ccacggcggt caaaaagggc gaccgttacc tggtcaatgg
      841 tcagaaggtc tggatatcgc gggtgcagca ttctgactgg atgattttgc tggcgcgcac
      901 cacggcgctg gccgaggtgc ggaaaaaatc cgaaggcatg tcgatcttca tggtggactt
      961 ggcgcaggcc gaaagaaacg gcatgacggt gcggccaatt gccaacatgg tcaaccacga
     1021 gaccaacgag ctgtttttcg agaatctgga aatccccgcc gagaacctga ttggccagga
     1081 aggccagggc ttcaagtaca ttctcgatgg cctgaacgcc gagcgcgccc tgatcgccgc
     1141 cgaatgcatt ggcgacggct actggtttct ggaccgtgtc acgtcttacg tcagcgagcg
     1201 ggcggttttt ggtcgcccga tcgggcaaaa ccagggcgtg cagtttccga ttgccgatgc
     1261 cttcatcgaa gtcgaagccg ccaacctgat gcgctacaag gcctgtgaac tgttcgatgc
     1321 cggccagcct tgtggcgcgc aggccaacat ggccaaatac ctggccgcca aggcaagctg
     1381 ggaagccgcc aacgcctgca tccagtttca cggcggcttc ggttttgcca acgactacga
     1441 tgtcgagcgc aagttccgcg agacccggct ctaccaggtg gcaccgattt cgaccaacct
     1501 gatttatgcc tacatcgccg agcatgtgct cggcctgcca aggtcattct gaaaccttgc
     1561 cgcactgacc accagcgctg tccccaacac ttcaagccag atgtctcggt tgaccgcctg
     1621 ttttcttctg taagttccta tgaatattga cttttttagt ttcgatgagg gtcacctggc
     1681 gggtcacagt gctacccgcc ggattgaagg ttccaggttc aaaggtgagt ttgatgacac
     1741 gacctcttga cggcatgacc gttgtttcgc tggagcacgc cattgcagcg cctttttgca
     1801 cccgccagct cgccgatctt ggcgccaggg tgatcaaggt cgagcggccc ggcgaaggcg
     1861 atttcgcccg ttcctatgac agccgggtca tgggtctggc ctcgcatttt gtctggacca
     1921 accgctccaa ggaaagcctc acgctggacc tcaagcagcc agcggcgctg gccgtgctgg
     1981 cgcaacttct ggggcaagcc gatgtgctgg tgcaaaacct cgcccccggc gcggcggcgc
     2041 gcatgggcct gtcgtttgcc gcgctgacag cggcgcaccc ccggctaatt gtgtgtgaca
     2101 tctcggaata tggcgaaaac ggcccgtacc gcgacaagaa agcctacgat ttgctgattc
     2161 agagcgaagt cggctttctg tctgtcactg gcacgcccga ggcgcccagc aaggcgggca
     2221 tttcggtggc cgatattgca gcgggcatgt atgcttacac cagcatcctg tcggccttgc
     2281 tgctgcgcgg caaaaccggg cagggcagcc agatcgacgt gtcaatgctg gagtcgctgg
     2341 ctgagtggat gggttttccg ctgtattaca cccatgcggg tgccgcaccg ccaccgcgca
     2401 ctgccgccgc acacgccagc atttacccct atggtccgtt tcaggtcggc gacggcgggg
     2461 tcgtgatgct gggcctgcaa aacgaacgcg agtggcaagc tttttgcgct ggcgtgcttg
     2521 aagatgccgc catcgccagc gacccgcgct ttgacagcag cgccaaccgt aaccagaacc
     2581 gtgatgcgct cagggcgctg atcctggcgg tgtttggcag attgacgact gcgcaagcgc
     2641 tggcgcgcct cgatcaggcg cagattgcaa acgcccgcat gaacgat
//
LOCUS       DAOSLL010000641         2682 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_33053_length_2682_cov_4.332699, whole genome shotgun sequence.
ACCESSION   DAOSLL010000641 DAOSLL010000000
VERSION     DAOSLL010000641.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33053_length_2682_cov_4.332699"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(85..1266)
                     /locus_tag="PLB25_20235"
     CDS             complement(85..1266)
                     /locus_tag="PLB25_20235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43935.1"
                     /translation="MSRFPRSLLTALMLSSSMAWAVLDCDIDGVSVNPGNGNTTKDKT
                     GIMRCRDRDSGQLQREQELRRGNFIGLVRFYKDGRLEKEFSVNEQGNRDGPGREFSTV
                     TGKVIREDISRNGSTVGLSRSFYPDGQPKRLSLRDEAGRELAAIEYTARGQLRELRCA
                     DKPLLGKEASETELCGFGLRGKSVTSALYGDKGELRTRLTHLEGARVASEGYWDNGKL
                     RSQEEIGTDGSQIERSFAPDGIKRQEIHWIKIDRGRQKELEQEFHESGSLVRERRWQL
                     GELVSEKSFYLNGQPRSDIRHFKREGTAATDVKEFHDNGKLAFQGAYLLDNRSSQRAI
                     GDHRRYDSNARLRQVRSYDGKGRLTREQELSESGQITRDDEVFEDGSRKALGVAPGRS
                     P"
     gene            1803..2546
                     /locus_tag="PLB25_20240"
     CDS             1803..2546
                     /locus_tag="PLB25_20240"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HOE43936.1"
                     /translation="MRKFLMVLVLGGILFAIGSAMAADVAAPRTVSVTGESEIRVVPD
                     EVVITLGIVVASKEIMEVKKLSDKRMKELLTITAAAGIPSRDVQTDFLNIDRDFTTDS
                     NGRRTFVGYVQRTTVEIFLRDLGKFEALLTAVLQAGIEHVHGIDFRTSELRQHRDQAR
                     SLAIKAAKEKATALAGELGEKIGKPHSIQEVQNYLFSTYRNWWGRSYQPMSQNVSQAV
                     SANTASQSNALAPGSLSIRASVNVSFELQ"
BASE COUNT          578 a          749 c          765 g          590 t
ORIGIN      
        1 accagcatta gttacaccca tgcagtgccg gagcggccca aaatgttgca ggaacatcac
       61 cgtcgaccgg gcttgcgaaa caactcaggg actacgccct ggtgctaccc cgagcgcttt
      121 tcttgagccg tcctcgaaga cctcgtcatc gcgggtgatc tgaccgctct cgctcaactc
      181 ctgctcgcgc gttagccgac ctttgccgtc gtaactgcgc acctgacgca aacgggcgtt
      241 gctgtcgtag cggcggtgat caccgatggc gcgttggctg gaacggttgt caagcaaata
      301 ggcaccctgg aaggcgagtt tgccgttgtc atgaaactcc ttgacatcgg tggcagccgt
      361 gccctcacgt ttgaaatggc ggatgtcgct gcgtggctgg ccattgaggt agaagctctt
      421 ttcactcacc agctcaccca actgccagcg gcgctcgcgc accagtgagc cgctctcgtg
      481 gaactcctgc tcaagctctt tttgccgacc tcgatcaatc ttgatccagt ggatttcctg
      541 gcgcttgatg ccgtccgggg caaagctgcg ctcgatttgg ctgccatcag tgccgatttc
      601 ttcctgcgag cgcagcttgc cattgtccca gtagccctcg gatgccaccc gagcgccttc
      661 aaggtgggtc aggcgagtgc gcagctcgcc cttgtcgcca tagagcgcgc ttgtcaccga
      721 cttgccgcgc aaaccaaagc cgcacaattc ggtttcgctc gcctccttgc ccaacagcgg
      781 cttgtcggcg cagcgcagct cacgcaactg gccgcgcgcg gtgtactcaa tggctgccag
      841 ctcccgcccg gcctcgtcgc gcaaactcag gcgtttgggc tgaccgtctg gatagaagct
      901 tcgtgacagg ccgactgtgc tgccattgcg ggatatgtct tcacggatca ccttgccggt
      961 tacagttgag aactcgcgac ccgggccgtc ccggttgccc tgctcattga cgctgaattc
     1021 cttttccagt cgtccgtctt tgtaaaagcg caccagaccg atgaagttgc cccggcgcag
     1081 ttcctgctcc cgctgcaatt gacccgaatc acgatcacga caacgcatga tgccagtctt
     1141 gtctttggta gtgttgccat tgccagggtt aaccgaaaca ccgtcaatat cgcagtccag
     1201 cactgcccat gccatgctgg aacttaacat gagggcggtt aagagtgacc gagggaatcg
     1261 agacatggcg gcgaaaccgg taaaaggtag acaaatgtta gccggtcacg ggcatcaatc
     1321 tagcgtacac gctggtggtg agctccgcgc aggcgtgcga ctgcaggcgc acccagttga
     1381 ccaggtcacc ggtggagtgg cgcttgtaga tttcttgaaa ccaaaccgtg ctgagcggaa
     1441 aaaaatcaga gcatgaaccc ctgaacatcg taaatttaca caatctaaaa gcaaagtcct
     1501 gacaatggca gcaatagatc gccatatcga tcggtatcga gtgtcatccg caggactggc
     1561 tgacctcgca tgaatcaggt gacacctact gcgggctttt tgttgtgtca gattcacgaa
     1621 ccgatgtcac cgatgtgggg ctcaaggaaa gacgtaccaa aacactgatt gcctcagttc
     1681 gcgccttatc gaacaacgtt gtggtggtga aatcgaccga agatacatgg cgaaagattg
     1741 ctcttgtgtg atgaaatgct gtgatccagg tggctgttca acatttgtca aaggagacga
     1801 aagtgagaaa gttcctgatg gtgttggttt tgggcgggat actttttgcc ataggctcag
     1861 cgatggcggc ggatgttgca gcgccaagaa cggtcagtgt gaccggcgag tccgaaatca
     1921 gggtggtacc cgatgaggtg gtgattacat tgggaatagt ggtggctagc aaggagatca
     1981 tggaggttaa gaaactcagc gacaagcgca tgaaggaatt gctgaccatt actgccgcag
     2041 cgggcatccc gtccagggat gttcagaccg atttcctcaa tatcgaccgc gacttcacca
     2101 ctgactccaa tggacggcga acctttgttg gctacgtgca gcgaaccacg gtggagattt
     2161 tcctgcggga cctcggcaag ttcgaggcgc tgctgactgc tgtgctccag gctggcatcg
     2221 agcacgtgca tggcattgac tttcgcacca gcgagcttcg gcagcatcgt gaccaagccc
     2281 gctctctggc catcaaggcg gccaaagaga aggccacggc cctggcgggc gaactgggtg
     2341 aaaaaatcgg caagccgcat tcgatccagg aagtccagaa ttacctgttc tcgacgtacc
     2401 ggaactggtg ggggcgcagc tatcaaccga tgtcgcagaa cgtatcgcaa gcggtaagcg
     2461 ccaacacagc cagtcagagc aacgcacttg ctcccggctc attgagcatc cgcgccagtg
     2521 tcaatgtgag ctttgagctt cagtagctta tgtgttttgt cgcactgact gggtcatggc
     2581 gggctttgga ttggttcaag accccggttt tcgggtaacg ctgatgtggc aattccgcct
     2641 tcagaagttc gagattctgg gtgtcattca cgaatatgac gc
//
LOCUS       DAOSLL010000642         2660 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_33392_length_2660_cov_3.807678, whole genome shotgun sequence.
ACCESSION   DAOSLL010000642 DAOSLL010000000
VERSION     DAOSLL010000642.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33392_length_2660_cov_3.807678"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PLB25_20245"
     CDS             complement(<1..358)
                     /locus_tag="PLB25_20245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011806534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HOE43937.1"
                     /translation="MTFPLNADLHCHSVVSDGTLSPQALAQRAKSNGITLWALTDHDE
                     VGGQAAALTAALSLGLDYLTGVEISVTFLTQTVHIVGLGFDADNLQLRQGLRQTRGGR
                     AQRAQEMAGQLALAGIN"
     gene            complement(622..2076)
                     /locus_tag="PLB25_20250"
     CDS             complement(622..2076)
                     /locus_tag="PLB25_20250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HOE43938.1"
                     /translation="MQALLNELITLVGSSNVLTEGDLSAWEQDWRKRTHGKALAVVRP
                     GNTDEVAQVVRACARHLQQHPACGISIVPQGGNTGLVVGSTPDASGRQIVLSLQRMNT
                     VRAIDAANLTITAEAGCILQRVQESALAAGFLFPLSLAAEGSCTIGGNLGSNAGGTQV
                     LRFGNARELCLGLEVVTPQGDIWHGLSGLRKDNTGYDLRHLYVGSEGTLGIITAATMK
                     LYPLPAAQTTAWAAVPSMQAAVDLLGLAQRHLGAGLTGFEVMGRFALSLVARHFANLH
                     VPLFADWPFCVLLEHSDHSSASHARAQFEQLLEVALAQGCVSDAVLAENLAQSKTLWQ
                     IRESISLAQALEGPNIKHDISVPISSIPAFVQATDALLQQAIPGVRLVNFGHLGDGNL
                     HYNAQAPLGIDAAVFLREQEPQVNRLVFDSVLQFGGSISAEHGIGSLKVDHLAHYKSP
                     VALQAMRAIKAALDPQNLLNPARVLAPIGTSELP"
     gene            complement(2057..2485)
                     /locus_tag="PLB25_20255"
     CDS             complement(2057..2485)
                     /locus_tag="PLB25_20255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HOE43939.1"
                     /translation="MKPSNLIAPTKPTLAVASTRVLAVGSLLGLIALGLAWELVLAPI
                     RPGGSWLALKVLPLCLPLAGLLKNRMYTYRWVSLLVWLYFTEGVVRGYSDKPPGNWLG
                     QIEVLLCLLLFVACALHVRLRLRKPQPFTGPMPRHASPAE"
BASE COUNT          518 a          846 c          818 g          478 t
ORIGIN      
        1 tgttgatgcc tgccaacgcc agttgcccgg ccatttcctg ggcccgctgg gcgcgcccgc
       61 cgcgcgtctg cctgagtccc tgacgcagtt gcaggttgtc ggcgtcaaag cccaagccga
      121 cgatatgcac ggtttgagtc agaaaagtga ccgatatctc aaccccggtc aggtagtcca
      181 gccccagcga cagcgccgct gtgagggcag cggcctgacc acccacttcg tcatgatcgg
      241 tcagcgccca taaggtgatg ccgttggact tggcgcgctg tgccagcgcc tgcgggctca
      301 gggtgccatc agagacgacg gaatggcaat gaagatcagc gttgaggggg aatgtcaccg
      361 accgatttta ggtgcattgg aaagcccggc gatcaacccg gcaatatcgt ttgatgcctt
      421 gcattgctga ctgcgcatgc aaacgatctg tggcacctgg tccgcgcgcg gcgcaggggc
      481 aagaactcga tcggttgaga tcgagacccc atgctcatcg caggctcgct gatcgggctg
      541 acagggggca ccgtcttgat tgcaaaattg atagcagctt acgcagacag gacgggggct
      601 acagggaaaa aatgcttgca actacggcaa ctcactggtg ccaatcggtg ccagcacccg
      661 tgccgggttc aacaggttct gcgggtccag cgcggctttg atggcacgca tggcttgcag
      721 cgctaccgga gatttgtaat gtgccaggtg atcgaccttc aggctgccaa tgccatgctc
      781 ggctgaaatt gagccaccaa actgcagcac cgagtcaaac accagccggt tgacctgcgg
      841 ctcttgctcg cgcaaaaaaa ccgccgcatc gatacccaac ggtgcctgcg cgttgtagtg
      901 caggttgccg tcccccaaat ggccaaaatt gaccagccgc acgccaggaa tggcctgctg
      961 cagcagcgca tcggtggcct gaacaaaggc cgggatgctg gagattggta ccgaaatgtc
     1021 atgtttgatg ttcgggcctt caagcgcctg cgccagcgaa atactttcgc ggatttgcca
     1081 cagggtttta ctctgcgcca ggttttctgc caacaccgcg tcgctcacgc acccctgcgc
     1141 cagcgccact tcgagcaact gctcaaactg tgcgcgggcg tggcttgctg acgaatggtc
     1201 cgagtgttcc agcagcacgc aaaacggcca gtcggcaaac aagggcacat gcaggttggc
     1261 aaaatggcgc gccaccaggc tcagggcaaa tcgccccatc acctcaaaac cggtcagccc
     1321 ggcacccaga tggcgttgcg ccaggccgag caggtccacc gctgcctgca tcgacggcac
     1381 cgccgcccag gcggtggttt gcgctgcagg cagcgggtac agcttcatgg tggcggcggt
     1441 gatgatgccc agtgtgcctt ccgaaccaac atacaggtgg cgcaggtcgt aaccggtgtt
     1501 gtccttgcgc aggccgctca aaccgtgcca gatgtcacct tgtggcgtga ccacttcgag
     1561 gcccaggcac agctcgcgcg cattgccaaa tcgcagcacc tgggtgcctc cggcgttgct
     1621 gccgaggttg ccgccaatgg tgcaggaacc ctcggcagcc agactcaagg ggaacaaaaa
     1681 acctgccgcc agggcgcttt cctgcaccct ttgcaaaatg cagccagcct ctgccgtgat
     1741 cgtcaggttg gccgcatcga ttgcccgcac cgtgttcatg cgttgcaggc tcagcacgat
     1801 ctgtcggccc gaagcgtccg gcgtcgagcc caccaccagc ccggtgttgc ccccttgcgg
     1861 cacgatgctg atgccgcacg ccgggtgctg ttgcaggtgc cgggcgcagg cccgcaccac
     1921 ctgcgcgact tcatcggtgt tgccggggcg caccaccgcc agtgccttgc cgtgcgtgcg
     1981 tttgcgccag tcctgttccc aggcgctcaa gtcaccctcg gtcagcacat tgctgctgcc
     2041 aaccagggtg atcagttcat tcagcagggc ttgcatggcg gggcatcggt ccggtaaaag
     2101 gctgcggctt gcgcaggcgc aggcgcacat gcagcgcaca ggcgacaaac agcaacaggc
     2161 acagcagcac ctcgatttgc ccgagccagt tgcccggcgg cttgtcgctg tagccgcgca
     2221 ccacgccttc ggtgaaatag agccagacca gcaggctgac ccagcggtag gtgtacatgc
     2281 ggttcttcag cagaccggcc agcggcaggc acaagggcag caccttcagt gccagccagg
     2341 atccgccagg gcggattggg gccagcacga gttcccaggc cagccccagg gcaatcaggc
     2401 caagcaggct gccaacggcc agaacgcggg tgctggccac cgccagggtc ggtttggttg
     2461 gggcgatcag attcgatggt ttcattgggg tggcatcata gcggccatga ccgaacgctc
     2521 acctcacccg caaaactggt cgcggcgact ggacctgttg ctgcagcagt tgtcccgctt
     2581 tccctggaaa aacaccgctt acacgctgcg cgagcgtttt cgtgaagacc gcctgggcct
     2641 gactgccagc agcctgacct
//
LOCUS       DAOSLL010000643         2657 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_33453_length_2657_cov_4.430054, whole genome shotgun sequence.
ACCESSION   DAOSLL010000643 DAOSLL010000000
VERSION     DAOSLL010000643.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33453_length_2657_cov_4.430054"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(936..2471)
                     /locus_tag="PLB25_20260"
     CDS             complement(936..2471)
                     /locus_tag="PLB25_20260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019425725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HOE43940.1"
                     /translation="MSDWTDGYVADIDYTYGYYPELNPLRARLALLNKGLALPGLSCA
                     CELGFGQGLSVNIHAAASGTQWVGTDFNPAQAGFAQSLAAASGSSARLHDQAFTEFAN
                     RSDLPDFDFVGLHGIWSWISDDNRRVIVDLLRRKLKVGGILYISYNTMPGWAAFAPMR
                     HLMALHAQTMGAPGIGTVSRVDNAIGFAEKLLATQPGFVRAQPQVTDRLAKLKGQNRH
                     YLAHEYFNADWQPMHFATMADWLAPAKLGYACSANYLDHFDGFNFKAAQQTLLKEIPD
                     AVLRETTRDFILNQQFRRDYWVKGPRRLAALDQAQSWRQQSVLLCTRRADVPLKIKTA
                     LGEATMSESVYAPILDLLADHQARTLTLIEQELKDQRVQLNQIKEAVLLLVSAGHMAL
                     AQDAPSIANAKPHTERLNAQLLGQARSSSDIAYLASPVTGGGVPLGRFQQLFLSAQKQ
                     GLKLPVEWADAAWQTLNAQNQRVIKDGKALDSAEKNLAELSSQAGSFAEQKLAVLQAL
                     QIA"
BASE COUNT          565 a          736 c          780 g          576 t
ORIGIN      
        1 cgctgtcacc caatgggtga acctcgccga agctaaagaa gtaaatgctc ataaggactt
       61 acagatgaaa acgagcggtc aactgaggaa tcaagggtga ggcatcatcc caaattcctg
      121 agttggatgc gcccgactgg accgctggcg gaggaggcag aagcagagcc cccaggcgcg
      181 ttgccagtgg ttcgatcagg tgggttgcgc tgtaacccaa taggtgaacc ctatcgaaac
      241 aagaaaagca actacttaca gggacttatt ccatttctaa atcatccatg tcgttgttat
      301 ctcgccttgc cttgcgtttg caatgtctgc gccaaggtgc ctagacagga atgatctaga
      361 aatggaattg cagtcgaaaa caagcggcca actgaggaat caaggttcat tgacccgacc
      421 atgccaaact tcggccgacg gcgcgcaaag agtgaagttt gcgtaatagt gcctgtaacc
      481 cttatagggc ttgcgctgac agctatgtag ttcgcagcaa aatgataggg tgatccagaa
      541 gtcaggagcg cagccaagtc actctttcac gcattgacga ccaagcgaga agaccaaagc
      601 caggttactc acttctggaa acaggggcag cgtgagccgc gcttcatcaa tcaggtggta
      661 acgcagtttg tctgggccag tacaacgcca aacccggcaa acccggcccg cgtccccact
      721 ggagaatcct ggatcaccgg gattcgaccc gctgtttcat gcacattttt gccctctagc
      781 ccttgtgcag catgcgtcgg cagctttcat attaatagca ttgaatgagc ccattggatg
      841 cccaatcagc agtcgcagcg cttcagtcga aattgtggct gtagcccccg ttgcatatgc
      901 ctggatagct attgacaata tagcaaaacg cggcctcagg cgatctgcag cgcctgcagc
      961 accgccagct tctgttcggc aaaactgcct gcctggctgc tcagctcagc caggttcttt
     1021 tcagccgaat ccagcgcctt gccgtccttg atgacccgct ggttttgcgc gttcaacgtt
     1081 tgccatgctg cgtcggccca ttcaacaggc agcttgagcc cctgcttttg cgcggacaga
     1141 aataactgct gaaagcgccc cagcggcacg ccgccgccag tgaccgggct ggccaggtaa
     1201 gcgatgtctg agctgctgcg cgcctggccc agcagttggg cattgagccg ctcggtgtgt
     1261 ggtttggcgt tggcaatgct gggggcatcc tgtgccaggg ccatatgacc ggcactgacc
     1321 agcagcagca cggcttcctt gatctggttg agttgcacac gctggtcttt gagctcttgc
     1381 tcgatcaggg tgagggttct ggcctggtga tcggccagca ggtccagtat cggggcataa
     1441 accgactcgc tcatggtcgc ttcaccgagc gcggtcttga tcttgagcgg aacatcggcg
     1501 cgccgggtgc atagcaagac cgattgttgg cgccagcttt gtgcctgatc cagggctgcg
     1561 agtcggcgcg ggcctttgac ccaatagtcg cgtcgaaact gctggttcag aatgaagtcg
     1621 cgggtggttt cgcgtaacac ggcatctgga atctctttca acagggtttg ctgcgccgcc
     1681 ttgaagttga acccgtcaaa gtgatcaagg tagttggcgg aacacgcata gcccagtttt
     1741 gccggtgcca gccagtctgc catggtggca aagtgcatcg gctgccagtc ggcgttgaag
     1801 tattcgtgtg ccagatagtg gcggttttgg cctttgagtt tggccagccg gtcggtcacc
     1861 tggggctgtg cgcgcacaaa cccaggctga gttgccagca gtttttcggc aaagccaatg
     1921 gcattgtcaa cccggctgac agtgccaatg cccggtgcgc ccatggtctg ggcgtgcaag
     1981 gccatcaggt ggcgcatcgg tgcaaaagcc gcccagcccg gcatggtgtt gtagctgatg
     2041 taaaggatgc cccccacctt gagcttgcgc cgcagcaggt caacaatcac ccggcggttg
     2101 tcgtcactga tccagctcca gataccgtgc aagccgacaa agtcaaagtc gggcaagtcg
     2161 ctgcggttgg caaattctgt aaaggcctgg tcatgcagcc gggcgctgct gccgctggct
     2221 gctgccaggc tctgggcaaa gcccgcctgc gccgggttga agtcggtccc aacccattgt
     2281 gtgccagatg cagccgcgtg aatgttgaca ctcaggccct ggccaaaacc cagttcgcag
     2341 gcgcagctta agcccggcag ggccaggccc ttgttcagca gggccaatct ggcgcgcaac
     2401 gggttgagct cagggtaata gccgtaggtg tagtcaatgt ctgcgacgta gccgtcggtc
     2461 cagtcactca tgcttgggtc ttctgtgatg gttgggtaag gatgtggtcg agaataactc
     2521 cccttttttg caccatggag ttggaatgca cggctagacg caagacgcgc cgtttagctg
     2581 gctaaacaag cgtcttgcaa caacgctgtg cgcccaaatc cgccggtgca aataggggaa
     2641 gttatttctg accacat
//
LOCUS       DAOSLL010000644         2629 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_33933_length_2629_cov_2.923077, whole genome shotgun sequence.
ACCESSION   DAOSLL010000644 DAOSLL010000000
VERSION     DAOSLL010000644.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33933_length_2629_cov_2.923077"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="PLB25_20265"
     CDS             <1..181
                     /locus_tag="PLB25_20265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464225.1"
                     /note="CcoO; FixO; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HOE43941.1"
                     /translation="WLMHAPVDAAVMPAHMRALRTVGVPYSDAEIAGSSEAVKGKTEM
                     EALVAYLQVLGTLVK"
     gene            197..340
                     /locus_tag="PLB25_20270"
     CDS             197..340
                     /locus_tag="PLB25_20270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HOE43942.1"
                     /translation="MDLDVNTLRSLVTVVSFLTFIGIIAWAFARKNASDFEKAANLPF
                     EQD"
     gene            365..1264
                     /gene="ccoP"
                     /locus_tag="PLB25_20275"
     CDS             365..1264
                     /gene="ccoP"
                     /locus_tag="PLB25_20275"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HOE43943.1"
                     /translation="MSDFTSNFWAMYVAGLTIFSIVACLVLLILTGKAKAMTAGDNTT
                     GHVWDGDLREMNNPLPRWWSYLFVITVIFAFGYLALYPGLGSNPGKLGWTSAGLHKAE
                     VAKGDAETAPIYAKFLDMKPEDVAKNAQAMAIGERLYSNNCAQCHASDAHGNKGFPNL
                     TDGDWLHGGTPEKIQETITGGRIGNMPPMAAAVGSADDVKNVAQYVLSLSGSPHDPAR
                     AALGKEKFGVCAACHGPDGKGMQAVGSPNLTDNIWLHGFGEKAIVDIVNGGKQNIMPP
                     QGQLLTKGQLHVLVSYIWGLSNK"
     gene            1384..1686
                     /locus_tag="PLB25_20280"
     CDS             1384..1686
                     /locus_tag="PLB25_20280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen fixation protein FixH"
                     /protein_id="HOE43944.1"
                     /translation="MAANFKPGLLMLANSVADEKPWWQFGHVWMVISGPLLVILACIV
                     TFYFVASSPNEIVTDEAYQQIMELKRAQGSKMMSGGDAPALQARNHAATGVVPVPK"
     gene            complement(1729..2448)
                     /gene="fnr"
                     /locus_tag="PLB25_20285"
     CDS             complement(1729..2448)
                     /gene="fnr"
                     /locus_tag="PLB25_20285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006296268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HOE43945.1"
                     /translation="MGKMNHETIKIACSNCNMRELCMPIGLSNADLARIDEVIGARRK
                     IKRSETLFHNGERFSNLYAIRTGFFKTCVTSEDGRDQVTGFQMAGEIIGLDGIVNDSH
                     TCDAIALEDGEICAMPFVQIENLSREVNVLQRHVHKIMSREIVREHGVMLLLGSMRAE
                     ERLAAFLLNLAQRLHARGFSQSELVLRMTREEIGSFLGLKLETISRTFSRFVEDGIVE
                     VRQRHVRILNTDSLQEIVNPR"
BASE COUNT          625 a          617 c          706 g          681 t
ORIGIN      
        1 atggttgatg catgcgcctg ttgatgcagc agtaatgcct gctcatatgc gtgcgttacg
       61 aaccgttggg gtgccttatt cggatgccga aattgctggc tcttctgaag cagtcaaggg
      121 taaaaccgaa atggaagcgc ttgtagctta cctgcaggtt ctcggaactt tggtcaagta
      181 agcgggaggg cttttgatgg acctcgatgt caacacgttg cgttcattgg tcacggtggt
      241 cagttttttg accttcattg gcattattgc ctgggcattt gcgcgcaaga acgcctctga
      301 ttttgaaaaa gcggctaacc tgcctttcga gcaggattga cgaattcacc gaataggaaa
      361 aaatatgagc gatttcacaa gtaatttttg ggcgatgtat gtcgctggcc tgacgatttt
      421 cagcattgtt gcgtgcttgg tgctgttgat cctgacgggg aaggcgaaag ccatgaccgc
      481 tggagacaac acaacgggtc atgtgtggga tggtgatttg cgtgaaatga acaatccgct
      541 gccgcgttgg tggagttatc tgtttgtgat aactgttatt tttgcgtttg gttatctggc
      601 actttaccca ggtttgggta gcaatccggg taagttgggc tggacatcag cgggccttca
      661 caaggcggaa gtcgcaaaag gtgatgcaga aacggctcct atttatgcca agttcctgga
      721 tatgaaacct gaggatgtcg ccaagaatgc gcaggcgatg gcaatcggcg agcgtcttta
      781 cagtaacaat tgtgcgcagt gtcatgcctc tgatgcccat ggcaacaagg gcttcccgaa
      841 tttgacagat ggtgactggc ttcacggtgg taccccagag aagattcagg aaacgatcac
      901 cggcggcagg atcggcaaca tgccacccat ggctgcagcc gttggcagtg ccgacgatgt
      961 caaaaatgtc gcgcaatatg tgttaagcct gtcaggtagt ccacacgatc ctgccagggc
     1021 tgcgttgggc aaagagaagt ttggcgtatg cgcagcttgc cacggccccg acggcaaggg
     1081 catgcaagca gtcggctcac ccaacctgac cgacaacatc tggttgcacg ggtttggtga
     1141 aaaagctatc gtggacatag ttaatggtgg caaacaaaac atcatgccgc ctcagggtca
     1201 gttgctgacc aaaggccagt tgcatgtgct ggtgtcctat atttggggct tgtcgaacaa
     1261 atagcagatt tttgagccat tcctttgcgg gagttgttgg tctgcgacaa gtgctcttaa
     1321 actgcgggtg gtcaaatgcg gccacccaat ttttttgctg caacaaagtg ctatgagcgc
     1381 tgtttggcag ctaacttcaa accgggacta ttaatgcttg caaattctgt tgcagatgaa
     1441 aaaccatggt ggcagtttgg ccacgtatgg atggtcattt ctggaccttt gctggtcatt
     1501 ttggcttgca tcgtcacttt ttactttgtg gcttcgagcc cgaacgaaat tgtcacggac
     1561 gaggcctatc agcaaatcat ggaattgaaa agagcacagg gaagcaagat gatgagtggt
     1621 ggagatgctc ccgcattgca agcacgcaac cacgccgcga caggcgttgt gccggttccg
     1681 aagtaaggca ggggttgaca ctggcgacgc aacagtcgct caaacccgct atctcggatt
     1741 gacgatttcc tgcaatgaat cggtgttgag aatgcgcaca tgtcgttgtc gcacttccac
     1801 gatgccgtcc tcaacgaacc tggagaaggt tcgactaatg gtttcaagtt taaggccaag
     1861 gaaactaccg atctcctcgc gggtcattcg caagaccagt tctgactgcg agaagcctcg
     1921 ggcatgcagc ctttgagcca gattcagtaa aaacgccgcc aggcgttctt ctgctcgcat
     1981 gctgccgagc aacaacatca caccgtgctc acggacaatt tcccgactca tgatcttgtg
     2041 aacgtgacgc tgcaatacat tgacttcgcg tgacagattc tcaatctgga caaagggcat
     2101 ggcgcaaatt tcgccatcct ccaatgcaat tgcgtcgcaa gtatgcgagt cattcacaat
     2161 gccatcgaga ccaatgatct ctccggccat ctggaagcca gtaacctgat cccgaccatc
     2221 ttctgatgtc acgcaggtct tgaaaaagcc cgttcgaatg gcgtagaggt tcgagaatct
     2281 ttcgccattg tgaaagagtg tttcactgcg tttgatcttt cgccgggccc caatcacctc
     2341 gtcgatgcgg gccaaatcgg cattgctgag tccaataggc atgcatagct cacgcatatt
     2401 gcagttcgag caggcgatct ttatggtctc gtggttcatc tttccaattt tgacattgtt
     2461 ctgaccaaaa acctgatctt agtcaaattt gtccttcgcg gctttttatt gtttggttga
     2521 tcgagatcaa gattgaaaca ttcgttcaca ggcatatttg gcccgattgt gaaggaacat
     2581 ttaatggttg aaagcgcgtt agtacccatt tccgaagatt tgattcgtc
//
LOCUS       DAOSLL010000645         2611 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_34234_length_2611_cov_4.821596, whole genome shotgun sequence.
ACCESSION   DAOSLL010000645 DAOSLL010000000
VERSION     DAOSLL010000645.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34234_length_2611_cov_4.821596"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            96..1175
                     /locus_tag="PLB25_20290"
     CDS             96..1175
                     /locus_tag="PLB25_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007855788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HOE43946.1"
                     /translation="MISLGSLFSRQPAPLLGLDISSSSVKLVELGRDKAGNLVLERCA
                     IEPLERGWISDGNIEKFDEVADALRRLVKKSGAKTKNVAMALPPSAVITKKIILPGGM
                     TDEELESQVETEANQYIPFPLDEVSLDFCVIGPSATSAGDVEVLIAASRREKVQDIQG
                     LAEAAGLTPVVVDVESYASRLAAGRLIENLPNNGSGLIVALFEIGALTTSMQVLRDDE
                     VLYDRDQAFGGAQLTQLIIRQYGFSQEEAESKKRSGELPEDYESSVLKPFLETMVQEL
                     GRALQFFFTSTPHNKVDYIMLAGGSAALPGLTAAVTQHTSFPCSLLNPFDGMEIGDGV
                     RLKRMTREAPSYLTSCGLALRRFAQ"
     gene            1172..1831
                     /locus_tag="PLB25_20295"
     CDS             1172..1831
                     /locus_tag="PLB25_20295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012746290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HOE43947.1"
                     /translation="MILINLLPHREAARKRRRDVFNISLGASALLGGLIAGAVFLWFQ
                     AAISVQQNTNGILETEIKKLESQIKDIAGLESEIAALRARQQAVEDLQSDRNLPVHLL
                     TELVNQLPDGVYISKMVQADKLVTLNGTAQSNERVSELLRNLSNNTPWFTKPELVEIV
                     AGSVALNREQKRVANFVIRVQLVRASDAEKAAAAAAVASDAIPAASPASAPASSVGVK
                     K"
     gene            1843..2505
                     /locus_tag="PLB25_20300"
     CDS             1843..2505
                     /locus_tag="PLB25_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HOE43948.1"
                     /translation="MVEKTSRSFDFSALQAKVQGQFQNLDPKDPSVWPSLPRYALFVV
                     TAIVVVTVLWFLWLTSSLEVLEQEQEKEVKLRADYKIKLAKAVNLDVLKKQREQVQQY
                     VTQLEKQLPGKSEMDALLSDINQAGLGRSLQFDLFRPGQVAVKEYYAELPITIKVSGR
                     YHDIGAFASDIANLSRIVTLNNLSISPKPDGTLSMDATAKTFRYLDPDEVATQRKAGG
                     KK"
BASE COUNT          589 a          616 c          768 g          638 t
ORIGIN      
        1 gaaaaaacct ttgcgggaca aggagcttac tgatagcatt caagtgaatt cttaagtatg
       61 actgtccatt tggcggtcaa tttcagggga cagttttgat ctcattgggg tcgctcttca
      121 gtcgccagcc ggcaccattg ctcggtctgg atatcagttc ttccagtgtt aagctggtcg
      181 agttgggccg tgacaaggcc ggtaatctgg tgcttgaacg gtgtgccatc gagcccctgg
      241 agcgaggctg gatcagtgat ggcaatattg aaaaatttga cgaagtggct gatgccttgc
      301 gcaggctcgt caagaaaagc ggtgccaaga ccaagaatgt agcgatggcg ttgccgccgt
      361 cagcagttat caccaagaag atcatcctgc ccggtggcat gacggatgaa gaattggaat
      421 cacaggttga gacagaagcc aatcagtaca tcccgttccc gctggatgag gtgagtttgg
      481 acttctgcgt gatcggtcca agtgctactt cagcaggcga tgttgaagta ctgattgcgg
      541 cctcccggcg ggaaaaagtg caggatattc agggtctcgc tgaagcggcg ggcctgacac
      601 ccgttgtggt ggatgttgaa tcgtatgctt cccgactggc tgctgggcga ttgatagaaa
      661 acctgcccaa caacggatca ggcttgatcg ttgcactttt cgagattggc gcactgacga
      721 ccagcatgca ggtgttgcgc gacgatgagg ttctttatga tcgggatcag gcttttggcg
      781 gagcccagtt gacgcagttg atcatccggc agtatggatt ctcacaggaa gaagccgaga
      841 gcaagaagcg cagcggtgaa ttgcccgagg actatgaatc ctctgtgctg aagccgtttc
      901 tggaaaccat ggtccaagag ttggggcgtg cgttgcagtt tttcttcacc agtacaccac
      961 acaacaaggt ggattacatc atgttggcgg gtggctcagc ggcacttccg ggtctgactg
     1021 cagccgtgac acagcacacc tctttcccgt gcagcctgct taatccgttt gacggcatgg
     1081 agattggaga cggggtgcgt ctgaagcgta tgacccgtga ggcgccctcc tatttgacat
     1141 cgtgcggctt ggcgctgcgg aggttcgcgc agtgatcttg attaatctgc ttccgcaccg
     1201 tgaggctgct cgcaagcggc gccgtgacgt cttcaatatt tcactcgggg cttcggctct
     1261 gttgggtgga ttgattgctg gcgcagtctt tttgtggttt caggccgcca tttctgttca
     1321 gcagaacacc aatggaattc ttgaaacaga gatcaagaaa ctagagagtc aaatcaagga
     1381 tattgctggt ttggagtccg aaattgcagc gttgcgcgca aggcagcagg ccgttgaaga
     1441 cctccagtct gatcgcaatt tgccagtgca cttgttgact gaactggtaa atcaattgcc
     1501 ggatggtgtt tatatttcaa aaatggttca ggcggacaaa ttggtgacac tgaacgggac
     1561 cgcccagtcc aatgagcgtg tctctgagtt gttgcgcaat ttgtcaaaca atacgccatg
     1621 gttcacgaag cccgagttgg tggaaatcgt ggccgggtca gttgcgctga atcgcgaaca
     1681 aaagcgcgtg gccaattttg tgattcgtgt tcagctggtt cgcgcgagcg acgctgagaa
     1741 ggcagctgcc gctgcagctg tcgcatcaga tgccattccc gcagcgtcgc ctgcgtctgc
     1801 accggcaagc agtgtcggtg tcaagaaata gctgggtgca ccatggtgga aaaaacttcc
     1861 cgctcgtttg atttttcggc tttacaggct aaggtgcagg gccaatttca gaacctggat
     1921 cccaaagatc cgtctgtttg gccttccctg ccgcgttatg ccctgtttgt ggtcaccgcc
     1981 attgttgtgg tcaccgtttt gtggttcttg tggctaacca gttcactaga ggtgctggaa
     2041 caggagcagg agaaagaggt aaagcttcgg gctgattaca agatcaagct agctaaagca
     2101 gtgaatctgg atgttttgaa aaagcagcgc gagcaggttc agcagtacgt aacgcagctg
     2161 gagaagcagt tgcccggaaa atccgagatg gatgccctgc tctctgacat caatcaggct
     2221 ggtcttggca gaagtctgca gttcgatttg ttccgcccag ggcaggtcgc cgtcaaggag
     2281 tattacgcag agctccctat tactatcaaa gtgagtggta gataccacga tatcggagct
     2341 tttgcgtccg acatcgccaa tctctcccgc atcgtgacgt taaacaattt gtccatcagt
     2401 cccaaacctg atggaacgct ttccatggac gctactgcca aaactttccg gtatctcgac
     2461 cccgatgaag tggctacaca gcgcaaagca gggggcaaga aatgagggcc gtctatggtt
     2521 tgctggcgat ggctggactt attggccttg cgggatgcgg tgcatcgcgg gaggatgata
     2581 ttcggcaatg gatggttgag gagcgaaatc a
//
LOCUS       DAOSLL010000646         2601 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_34412_length_2601_cov_6.041634, whole genome shotgun sequence.
ACCESSION   DAOSLL010000646 DAOSLL010000000
VERSION     DAOSLL010000646.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34412_length_2601_cov_6.041634"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(5..289)
                     /locus_tag="PLB25_20305"
     CDS             complement(5..289)
                     /locus_tag="PLB25_20305"
                     /inference="COORDINATES: protein motif:HMM:NF019605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE43949.1"
                     /translation="MNPIESKLYLDSFLDNNIQYNLSDYHPKEKPTSIFLTGSTGFLG
                     IHLLAELLSSSKASIHCLVRGLDADDGRRRIIEKMGSSSFCVEVALKNKM"
     gene            672..1403
                     /locus_tag="PLB25_20310"
     CDS             672..1403
                     /locus_tag="PLB25_20310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011483226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43950.1"
                     /translation="MQSLPELKRHHAKRLREVYRSAGWPCLDVIEIELLAAGLLERHS
                     LDDHAHVRVTDAGIQYLASAAHSNRQAMSAHEALVDQVTQVLLRDGRLVWKNLNLRAW
                     VAPNDDTPGRWKMCLPDVFSIRNSSVQAYLEPIVHEIKVSRADLLGDLKRSDKRAAYL
                     DLGGQCWYVLGCDRSGHPIAKPNEIPLECGVMVYQGNQLEVMRMAPKRPAPELPFGVW
                     MALAKATPMQMSGLDNCQGTLPYQS"
     gene            complement(1827..2303)
                     /locus_tag="PLB25_20315"
     CDS             complement(1827..2303)
                     /locus_tag="PLB25_20315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin family protein"
                     /protein_id="HOE43951.1"
                     /translation="MTINESESTHVTATQGLAYNTLRHLIHWKPAMLVGEAEIDIEHE
                     RIFALAERASVLSRDHKNAAELVRAFSDFGDLLFSHFEHEERKLSNANYGGLNQHIAQ
                     HSAMKSEFEFIRQRLMDNGVGWAYEEQALVVLNFMIGATVGHVLQSDSHFASSIKS"
BASE COUNT          647 a          656 c          671 g          627 t
ORIGIN      
        1 actactacat tttatttttc aatgcaactt ccacacaaaa cgacgaagaa cccattttct
       61 ctataatcct acgtcgacca tcatctgcat ctaaaccacg aaccaggcaa tgaattgatg
      121 cctttgaact gctcaaaagt tcagccaata aatgaatacc caaaaatcct gtcgatcctg
      181 ttaaaaaaat tgaagttggt ttttctttcg gatgataatc agaaagatta tattgaatat
      241 tattgtcaag aaagctatct aaatataatt tagattcaat ggggttcatg agctttccaa
      301 atcctgcaaa tcatttcacg tttagctggt gagggacttg cagaattccg agtaccagtc
      361 taattggctg caattagagg caaaaaattg agggtagccg tgtccaagta cgggaaaatc
      421 caagtatcca aaaaagagat cgctggctac ccatgaatac tactctgagt cccgtcaccg
      481 tccaaagatt ttgcgccttg taagaagaac tcatcgggtt ggttgaacaa gactacacgg
      541 gactaacgcc aaagtcaatc caccgaagct aagcctatct ggtcgatgaa gcaggagcgc
      601 cggaccccgg cttacaccac ttgcctggca gacatgccaa cagcaaaagc ctgagttgag
      661 gagcacacaa catgcagtcc ttgcctgaac tcaaacgtca tcatgccaaa cggctgcgcg
      721 aggtgtatcg ctctgcgggg tggccttgcc tggatgtgat cgagattgag cttctggcag
      781 ccggactgct ggagcgccac agccttgatg accatgccca tgtgcgcgtg acagatgccg
      841 gtatccaata cctggccagt gccgcccata gcaatcggca agcgatgtca gcccatgaag
      901 ccctggtgga tcaggtgacc caggtgttgc tgcgcgacgg gcgattggtg tggaagaacc
      961 tgaacctgcg tgcctgggtc gcaccaaatg acgacacacc cgggcgctgg aagatgtgcc
     1021 tgcccgatgt gttctccatt cgtaacagca gtgtgcaggc gtacctggag cccatcgtgc
     1081 atgagatcaa ggtcagtcgt gccgatttgc tgggagatct caaacggtcc gacaaacgcg
     1141 ctgcttatct ggacctgggc gggcaatgct ggtatgtgct gggttgtgat cgtagtggcc
     1201 atccgattgc caagcccaac gagattccac tggagtgtgg ggtgatggtg taccagggca
     1261 accagttgga ggtgatgcgg atggcaccca agcggcctgc cccggagttg cccttcggtg
     1321 tctggatggc gctggccaaa gccacaccga tgcagatgtc tggcttggac aattgccagg
     1381 ggacgctccc atatcagtct tgatagcgat ttcgcagtgg cggcgactgg tgtgcctaaa
     1441 cctctgagaa tgacttgtct caattttgtt gactgcggat ggtcgataca tttctcaggg
     1501 atggcaactg gtctttgctg aaaacatgga ctagcggtgc ctgcggcaga atctgagggt
     1561 tgtccggcgg caattttgct ggcaggcaat gacagcttca gacccgcagg cgtcattcga
     1621 cttcccgaac caatggctcc aaagttgaca ttggcatcag cttcctgagc ggcaacaatg
     1681 tgccgatgat tttgatggcc gcagccccga cattcaatca gatgtcgcat tattgggcgc
     1741 atacaccctg aagcagtcgg tggtcgacgc aatttctcca ctgcggttac tcaaatgcag
     1801 atggcaaccc cggatacatg tccccttcag gacttgatgc tggatgcgaa gtgcgagtca
     1861 ctttggagta cgtgaccgac tgttgcgcca atcataaagt tgagcaccac gagcgcctgc
     1921 tcttcatagg cccagccgac gccgttgtcc ataagtcgtt gacggatgaa ttcaaactcc
     1981 gatttcatcg cactatgctg tgcgatgtgc tgattgagac ctccataatt ggcgttggac
     2041 agtttgcgct cctcgtgctc aaaatgactg aaaagtaggt cgccgaaatc gctaaatgcc
     2101 ctgaccagtt cggctgcatt cttatgatca cgtgacagca cactagcgcg ctcagccaag
     2161 gcgaatatcc tttcatgctc gatgtcaatt tctgcttcac cgaccagcat tgccggtttc
     2221 cagtgaatca aatgacgcaa cgtgttgtag gccagcccct gggtggcagt aacatgtgta
     2281 gactcagact cgtttatggt catgcccgac tcctgcgttt gagtaaatga ttgatacagt
     2341 ttatatcgtt taatttgaaa agccaagagc gaaacgatcc tttaacctgt ggcaaaaatt
     2401 ctttacgcag agtgcaagaa ttttccatcc aatttcgagg gcctctcgac ggctgatctg
     2461 ccgcctaatt catgaaattg agtcactcaa aagcggacag tggtcagcgg cggcttgcgc
     2521 tgcagtgctg accgccagtt tgacaaggtg attttcaccg ctgaatggcc gctcctgaga
     2581 ttcgggtgtc aaaaacgaaa g
//
LOCUS       DAOSLL010000647         2592 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_34573_length_2592_cov_3.751281, whole genome shotgun sequence.
ACCESSION   DAOSLL010000647 DAOSLL010000000
VERSION     DAOSLL010000647.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34573_length_2592_cov_3.751281"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..937)
                     /locus_tag="PLB25_20320"
     CDS             complement(50..937)
                     /locus_tag="PLB25_20320"
                     /inference="COORDINATES: protein motif:HMM:NF016648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HOE43952.1"
                     /translation="MPTAWAADFIYTVQPGDHPWNLAQRYLKRPAFSSRLLQLNQIAD
                     DHRLMPGTRLRIPQEWIRLQSAQVHLLAVYGETTLGQGNSLPRAAVPGELLQANAVLR
                     TGASGSATLGFADDSRVLLGHDSELRMAQARSGTLGRASVVELVLLQGSLENQITPVG
                     NSGGRFEIQTPAAVAAVRGTHFRVRSQRQQLHTEVLSGAVAVSNVRGKASAGAGQGLV
                     TENDQPPGSVAALLPAPSLDGVPALVQRLPIDLPLPSLAGAVTYRTQLAPDASFSVVV
                     SDETSSAPRLRAGDLADGN"
     gene            complement(1486..1847)
                     /locus_tag="PLB25_20325"
                     /pseudo
     CDS             complement(1486..1847)
                     /locus_tag="PLB25_20325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870937.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2140..2322)
                     /locus_tag="PLB25_20330"
     CDS             complement(2140..2322)
                     /locus_tag="PLB25_20330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43953.1"
                     /translation="MLNAMVLRGFVVLTHARYTLLGQTLLGQLRAHWRDRRPTTWLFC
                     NHTGIQPASIESAQRA"
BASE COUNT          569 a          743 c          718 g          562 t
ORIGIN      
        1 ccacgtttcg gcaggtttcc ccagggagat tggcgttttc gttcacgcac taattaccgt
       61 cggccaggtc acccgcgcgc agacgcggtg cactgctggt ttcatctgat accaccacgc
      121 taaaactggc atcaggtgcc aattgcgtgc ggtaggttac agcgccagcc aagctgggca
      181 aaggcaaatc gattggcaag cgctgcacca gcgctggcac gccatcaagg ctgggtgctg
      241 gcaacaaggc cgcgaccgag cctggtggct ggtcgttttc ggtcaccagg ccctgaccag
      301 caccggcact ggccttgccc cgcacattgg agacggcaac cgcgccactc aggacttcgg
      361 tgtgcaattg ttggcgctgg gaacgcaccc ggaaatgggt gccccggacc gctgccacgg
      421 cagccggtgt ctgaatttca aagcgaccac ccgaattgcc caccggggtg atctggtttt
      481 ccaggctgcc ttgcaacagc accagttcga ccacgctggc cctgcccaga gtgccagacc
      541 gggcctgtgc catacgcagt tcagaatcgt gacccaacaa cacccgcgag tcgtcggcaa
      601 acccaagcgt tgcgctgccg ctggcaccgg tgcgcaacac cgcgttggcc tgcaacaact
      661 cgccgggtac ggcggcgcgc ggcagactgt tgccctggcc caaagtggtt tcgccataga
      721 ctgccagcag gtgtacttgc gccgactgca gcctgatcca ttcctgcggt atgcgcagac
      781 gcgtgccggg catcagccga tgatcgtctg caatctggtt taactgaagc agccgactgc
      841 tgaaagcggg gcgtttgaga tagcgctgcg ccaggttcca gggatgatcg ccgggctgaa
      901 cggtatagat gaaatcagcc gcccatgcgg tgggcataac tgcgatgaga acgaaaccca
      961 attcgaggca cttctgatgt tttcgtccaa cgattgtcaa caagttcttt gtgctacgca
     1021 tttcccaagt ctccagtgtg aaccctgtgc cggaaccacg actccatggc ctcgtattga
     1081 tggacccgag gctacccatg gcgacgaaag atagtcgcaa tttacttggg attgctgcgc
     1141 catccatgca atctgaaggc gtgaatgacg gatcatgtct gacatgacag ccaacgggca
     1201 ttgatcttac tctcgttgac ttggtgttga gcgtttgaaa accgtcaatg gcaaaagctc
     1261 cagaacacgc gtttcagacc cctgtgctgc gtcttaggtt gctggccctg atcgggagac
     1321 gcgcatatca agtcattcgc atagcgtttt ttgccgggtg cgattcaagc tgatgtgcca
     1381 gttgtgcggg tcgggttgaa accaggcaag aggcgttgcc aattcgtttt ctatccaaaa
     1441 aagtcggctt cttgatttgg tacccggtct gccgtccaag gcccgttaaa tcaaattgcg
     1501 catcgccctg gcggtctcca acgacaccgg aagcacccgg gccaccgcat cttctggctc
     1561 tcgtggccca ttggcatggt gacgttcagt gcacacccta tgtgacaccg aaggtcaatc
     1621 agacgcacgg cgacaccccg gcaagttgtc tcaagctgct actcggaaat tgcacaacca
     1681 cgttgcctga tgcgcgcaaa acaggctcta tcgttaacgt ccatcacgtt gacataggcg
     1741 ttctcctgag tgccagaggt gaacctccgc tgaaacggct gcacgatgcg cgggttgaca
     1801 ttgtcaaagg ccacaatgac agccagaccc ttctgcggac cggcaatcca ttcacgcttg
     1861 tcaagtgcag cgcctgaagc gggttatctc acgatctcaa agttgctggg catcgtgttg
     1921 gtttccttaa gcgcgatgcg catcaaacct accaacatgt tgtcgatcat tgccgacagt
     1981 ctcgggcggc agacaacctg ttttgaacct gttggtggcc atggttgttc gccaaaggca
     2041 aacaaattca tcgcttgatg cagacgtaat ttgttgtctt caaacgattt gtaggtccga
     2101 caggctgcta gaaacgcgta gaacgcccgc tcaaccgctt taggcgcgct gggctgattc
     2161 gatgctcgcc ggttgtatgc cggtgtggtt gcaaaacagc caggttgtgg ggcgacggtc
     2221 tctccaatgg gcgcgcagtt gccccaacaa ggtctgaccc agcagggtgt agcgagcgtg
     2281 ggtgagtaca acaaagcctc gcagcaccat tgcgttgagc atgcgttggc gttacggtgt
     2341 catgacaaat taccttcaaa accagggaac atccccagtt cgaaggtttc accccaaaca
     2401 ccttttgcgc aagtcgcgct caaacaaaaa cgcggtggtg gcctgggcag accaaccacc
     2461 gcgtcagcgg ttgagtttga caattattaa aacatatcga ttatgttatt acagtgttta
     2521 acctgggata agcattacca tgtttcgcca aatggccttg cttcgacttc aaaagaccac
     2581 gcagaacgag gc
//
LOCUS       DAOSLL010000648         2587 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_34653_length_2587_cov_5.590047, whole genome shotgun sequence.
ACCESSION   DAOSLL010000648 DAOSLL010000000
VERSION     DAOSLL010000648.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34653_length_2587_cov_5.590047"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..1199)
                     /locus_tag="PLB25_20335"
     CDS             complement(105..1199)
                     /locus_tag="PLB25_20335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HOE43954.1"
                     /translation="MRIVLMGAGHIGQTIARLLSQSGDYQVKVVDCSVVALKALSHLQ
                     VETALIDTSDANELRACMTGFDAVINALPYHLALTVASLAKAAGCHYFDLTEDMVATR
                     EIMQLARGASTAFMPQCGLAPGFIGIVAHHLSQKFSYLHEVKMRVGALPAFPTNSLKY
                     NLTWSVDGLINEYCHPCEAIREGNVIEVLPLEGLEHFSLDGTEYEAFNTSGGLGTLCD
                     TLKGKVQTLDYKTVRYPGHCGIMKMLLQELGMRSDQDTLKAILRRSIPLTMQDVVLVF
                     VTVSGECDGKFVQEVFARKIFADRSEVNPMSAIQITTAAGICAAVDMFREGKLPQSGF
                     IRQEQVALPDLLANRFGSAYQQSRQVESIG"
     gene            1662..2135
                     /locus_tag="PLB25_20340"
     CDS             1662..2135
                     /locus_tag="PLB25_20340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HOE43955.1"
                     /translation="MPLVASPTLPPPPALRDALDRELLALLQVNARESAANLARKLGT
                     ARTTVLSRLSRLEREGIIAGYTVRLAQDLLNQSLQAFVGLTVQPKAGRLIEARLERMP
                     ELRQLCAVSGEFDYVLLLRAESAVRLNALLDEIRNLEGVVKTTTSVALAWKIERI"
     gene            2271..>2587
                     /locus_tag="PLB25_20345"
     CDS             2271..>2587
                     /locus_tag="PLB25_20345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HOE43956.1"
                     /translation="MNHNISLIGAPTDIGAGSRGASMGPEALRVADIVPVLQSHGLQV
                     IDRGNLTGPHNPWLAPVDGYRHLDEVVAWNTTVHEAVYAELALQRLPILLGGDHCLGL
                     GSI"
BASE COUNT          549 a          773 c          757 g          508 t
ORIGIN      
        1 gggaaaccaa agcgggtgtg gcggcaacaa ttgccgcagg tcagacctgc caccagaggc
       61 ggcaacaggc ccggcagcca gtctgaaaaa tggcgggtct ggctttagcc gatcgactcc
      121 acctggcgcg actgctggta ggcgctgcca aagcggttgg ccagcaagtc tggcaatgcc
      181 acctgctctt ggcggataaa gcccgactgc ggcagcttgc cttcgcgaaa catgtccacg
      241 gcagcacaaa tgccggccgc cgtggtgatc tggatggcac tcatcgggtt gacctcactg
      301 cggtcggcaa aaatcttgcg ggcaaaaacc tcctgcacaa acttgccgtc acactcgcca
      361 ctgacggtga caaaaaccag caccacgtcc tgcatggtca gcgggatcga gcggcgcaag
      421 atggccttga gggtgtcctg gtcagaccgc atgcccagtt cctgcagcag catcttcatg
      481 atgccgcagt ggccggggta gcgcacggtc ttgtagtcca gcgtctgaac cttgcctttc
      541 aaggtgtcgc acagcgtgcc caagccaccc gaggtgttga aggcttcgta ttcggtgccg
      601 tcgagcgaaa agtgttccag cccttccagc ggcagcactt cgatgacgtt gccttcacgg
      661 attgcttcgc aggggtggca atactcgttg atcaggccat caacgctcca ggtcaggttg
      721 tacttgagcg aattggtggg aaacgccggt agcgcaccga cccgcatctt gacctcatgc
      781 aggtagctga acttctggct caggtggtgc gccacgatgc caataaagcc gggtgccagt
      841 ccgcattgcg gcatgaaggc ggtgctggca cccctggcca gttgcatgat ctcgcgggtg
      901 gccaccatgt cttcggtcag atcaaaatag tggcaacccg ctgctttggc caggctggca
      961 acggtcagcg ccaggtggta tggcagggca ttgatgaccg catcaaaacc tgtcatgcaa
     1021 gcgcgcaatt cgttcgcatc agaggtgtcg atcagcgccg tttcgacctg caggtgcgac
     1081 agcgccttga gcgccaccac gctgcagtcc accaccttga cctggtaatc gccactttgc
     1141 gacagcaggc gggcaattgt ttggccaatg tggccggcac ccatcagaac gatacgcatg
     1201 gaattctcct gaaaaaaatc atcaacagac ttaggtgtca agcaaaaaca acttgaacag
     1261 ttcggccgca ctggccagcg caatgcgttg ttgcaaaccg cttgtgtcgc cagctaaaca
     1321 agcggtttgc aacgacactg tgcgcccaaa tccgccggtg cagataaggg aatatatttc
     1381 tgaccacatc ctatgccgga cgagccggaa tcgatgagcc ttcgtcgcga gggcgcgact
     1441 cctacacaaa cagtcggcgc caatttgaat ttgtaggagt cgcgccctcg cggcgaatga
     1501 ctttgaatat ttgaattctt cgcatgatat gcaaagactt gacttgttag agattaagtt
     1561 taggggcagt tgttgtcaga accaccgggt gcaatgacga aaaactattc ctgaatgtca
     1621 caatggcatc tttttcgtca tatcgacggt tccaagtccc catgccactt gtcgcatcac
     1681 ccacattgcc cccaccgcca gccctgcgcg acgcacttga ccgcgagttg ctggcgctgc
     1741 tgcaagtcaa cgcccgcgaa tctgccgcca atctggcgcg caagctgggc accgcacgca
     1801 ccaccgtgtt gtcgcgcctg agtcggctgg agcgcgaagg catcattgcc ggttacaccg
     1861 tgcggctggc gcaagacctg ctgaaccaga gcctgcaggc gtttgtcggc ctgaccgtgc
     1921 agcccaaagc cggtcgcctg atcgaagcgc ggttggagcg catgcctgaa ctgcgccagc
     1981 tctgcgccgt cagtggcgag tttgattacg tgctgctgct gcgcgccgaa tctgccgtgc
     2041 gcctgaatgc cttgctcgat gaaatccgca acctcgaagg ggtcgtcaag accaccacct
     2101 cggtcgcgct ggcctggaaa atcgagcgca tttgaagcct ggcgggaccg atcaagacca
     2161 cttgagccca gcaagcaaca gttgcccggc tgcccgatga ttgcctcatg ctgaatggcc
     2221 aacgaatttc agttaaaacc gcagttattg ccatcaaccg gacccgcatc atgaaccaca
     2281 acatcagcct gattggcgca cccaccgaca ttggcgcggg cagccgtggg gccagcatgg
     2341 ggccggaagc cctgcgggtg gccgatatcg tgccggtgct gcagagccat ggtctgcagg
     2401 tgattgaccg gggcaacttg accggtccgc acaacccatg gctggcaccg gtggacggct
     2461 accggcatct cgatgaagtg gtggcctgga acaccaccgt gcatgaagcg gtctatgccg
     2521 aactggcgct gcagcggctg ccgatcttgc tcggtggcga ccattgcctg gggctggggt
     2581 caatcag
//
LOCUS       DAOSLL010000649         2581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_34737_length_2581_cov_4.838480, whole genome shotgun sequence.
ACCESSION   DAOSLL010000649 DAOSLL010000000
VERSION     DAOSLL010000649.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34737_length_2581_cov_4.838480"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            100..1284
                     /locus_tag="PLB25_20350"
     CDS             100..1284
                     /locus_tag="PLB25_20350"
                     /inference="COORDINATES: protein motif:HMM:NF033519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HOE43957.1"
                     /translation="MTFFKKTAAALKGSARRKFMANAVEQLGRGGQTQAEVKLGWART
                     SIRLGQREVKTGIRCVPACNLRGRKPVVFHLPNLLEDIRQIVDGFSQTDPQFRHKRLY
                     TRLTVSELRRQLIAQKNYAPEALPGDEALRLRMNRLGFILKKVEKSKPLKKIPQTNAI
                     FEQLAVANKSAHANEEVLRISMDAKAVVKMGELSRDGRTLAPTVALDHDFKGIMVTPM
                     DFFLSQHDELYVYMVTRSTSDCQVDCLQDFWHSAKHKFPLVNTLAINIDNSPDCNSYR
                     TQFMKRLVDFSQESGLTFDLAYYPPYHSKYDPVERCWGILENHWNGSILDSIEAVVGC
                     ASSMTWKGLHPVVKLIDTVYKKGVTLGKVAMRAVEAKLQRLPALPRWFITIQPGYVPE
                     RQ"
     gene            1721..2305
                     /locus_tag="PLB25_20355"
     CDS             1721..2305
                     /locus_tag="PLB25_20355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43958.1"
                     /translation="MLLSILVAVAAQAAQPIEVRFKVIDSAGQAIIGAPIRLVLALGQ
                     TWHLPEAGMRATTGSGGEVNWTANALPERRRRKLPTNFFTQVLAPAEDTIHLAVGVEL
                     TYLARAWLVVGAGDRFDNGTTAQLDGLKIYGRDQSGNFSLPAVQKEGAWYLPGVPGAL
                     TTPGFLVHRLAIAPAERGWTMEFTVQRASEPVAR"
     gene            2379..>2581
                     /locus_tag="PLB25_20360"
     CDS             2379..>2581
                     /locus_tag="PLB25_20360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43959.1"
                     /translation="MLTKMPSGDSTRVWFPEMLDEMQKQWSESLIDLFITHDNHLPPR
                     ALLLERVDWRDDEWLQSGWTRQQG"
BASE COUNT          620 a          714 c          712 g          535 t
ORIGIN      
        1 gagcactgaa ccaaagcagg tgaaaaggct ataaatgcca tccaatcgac acttgaaaac
       61 tattcaagag tatgtagcct gaatctcact gaaaccgtaa tcaccttctt taagaagacc
      121 gcagcagccc tcaaaggcag cgcacgccga aagttcatgg ccaatgctgt cgagcaactc
      181 ggccgtggtg gccaaacaca ggcagaggtc aagttgggtt gggcgcgcac ctccatccgc
      241 ttgggccaac gtgaagtcaa gacaggcata cggtgcgttc ctgcttgcaa cctacgcggt
      301 cgcaaaccag ttgtatttca tctgcccaat ttgcttgagg atatccggca aatcgttgac
      361 ggtttcagtc agactgatcc ccagtttcgc cacaaacggc tctacaccag actcacggta
      421 tccgagttgc gtcgccaact catcgcgcag aagaactacg ccccagaggc gttacctggg
      481 gatgaagcac tgcgacttcg gatgaaccga ctggggttca ttctgaagaa agttgagaag
      541 tccaaaccgc tgaaaaagat tccacaaacc aacgccatat tcgagcagct cgcagtagcc
      601 aataagagcg cgcacgccaa tgaggaagtc ctgcgcatct cgatggatgc caaggcagtt
      661 gtcaagatgg gggagctgtc gcgtgatgga cgcaccctcg caccgacagt ggcactggat
      721 catgacttca aaggcatcat ggttaccccg atggatttct ttctatctca gcatgacgag
      781 ctgtatgtct acatggtgac acgttcgaca agcgattgcc aggtcgactg tctgcaagat
      841 ttttggcaca gcgccaagca caagtttccc ctggtgaata ctcttgccat caatattgac
      901 aacagtcccg actgcaacag ctaccgcacc cagttcatga aacgtttggt ggacttctcg
      961 caggaatccg gactcacctt cgaccttgcc tactatccac cgtatcacag caaatacgac
     1021 ccggtcgagc gctgctgggg catcctggag aatcactgga acggcagtat tctcgatagc
     1081 atagaagcag tcgtgggatg tgcctcctca atgacatgga agggcctgca tccggtcgtc
     1141 aagctgatcg ataccgtcta caagaagggt gtcacgttgg gcaaagttgc tatgcgggca
     1201 gtggaagcaa aactacagcg actgccagca ctgccacgct ggttcatcac cattcagcca
     1261 ggatatgtac cggagcggca ataagtgggg catcctttag tgtttgccca caccactgag
     1321 caattcgttg gcgcacagcg ctggtacgat gctatgcatg gaaacaatgg ggcagcgcgg
     1381 gatcccgcga gctaaggaaa cgcggtgcca ctgtgcgcga ggcttggaac acaagcaaga
     1441 gcgcgcatgg gccatggcgc ctgagcaaaa cgccagcatt gagtctggca ctgcctgcaa
     1501 agttgtttcg tcaaatgggg ttgcccgaat tggctccggc caaggcagca tgagatcaac
     1561 aatgtgtcaa ggaaccgccg gaaacgtgac ccgtatgtcc ggtggtgtga gagggagggg
     1621 ccgtgaggct tcttcctatc tcgattgggc attggaatca acgacacgcg cgcgtcctgc
     1681 atcaatgact cgttccttgc tccttcgaca cctgagtggc atgctgctat cgattctcgt
     1741 ggctgtcgcc gcacaagcgg cacaaccgat tgaggtccgc ttcaaggtca tcgactcggc
     1801 tggccaggcc atcatcggtg cgcccatccg gttagttctg gccctgggtc agacctggca
     1861 ccttcctgag gcgggcatga gagcaacgac cggcagtggg ggcgaagtga actggaccgc
     1921 aaatgccctg cctgaacgta ggcgaaggaa gctaccgacc aacttcttca ctcaggtcct
     1981 tgctccggca gaggacacca tccacctggc agtcggagtc gaactcacct atctcgctcg
     2041 cgcttggctt gtcgtcggcg ctggtgatcg cttcgacaac ggcacaaccg cacagctgga
     2101 cggactcaaa atttacggcc gcgatcagag tggcaacttc tcgcttcccg cagtccaaaa
     2161 ggaaggcgct tggtacttgc ccggggtgcc aggggcactc acgacacctg ggttcctcgt
     2221 gcaccgccta gcaatcgcgc ctgctgaacg aggttggacc atggagttca ctgtgcaacg
     2281 agcgagtgag cccgtcgctc gctgagttga attgccgcgc cgatgcctac ccattcatca
     2341 agcgggacag gactgaagcg gcctgtcccg cttgatgaat gttaaccaaa atgccttctg
     2401 gtgattccac acgagtctgg ttccctgaaa tgcttgatga gatgcaaaag cagtggagcg
     2461 agtcacttat tgatcttttc ataactcatg acaaccattt gccgcctcgt gcgttattgc
     2521 ttgaaagagt tgattggaga gatgacgaat ggctgcaaag cggatggacg aggcaacaag
     2581 g
//
LOCUS       DAOSLL010000650         2581 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_34749_length_2581_cov_2.699921, whole genome shotgun sequence.
ACCESSION   DAOSLL010000650 DAOSLL010000000
VERSION     DAOSLL010000650.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2581)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2581
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34749_length_2581_cov_2.699921"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PLB25_20365"
     CDS             complement(<1..234)
                     /locus_tag="PLB25_20365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013517177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HOE43960.1"
                     /translation="MSAFLNETVLSVHHWTDRLFSFTTTRDPTLRFSNGHFTMIGLRL
                     EGGKPLLRAYSIVSANYEEHLEFLSIKVQDGPLT"
     gene            complement(469..2343)
                     /locus_tag="PLB25_20370"
     CDS             complement(469..2343)
                     /locus_tag="PLB25_20370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020704045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HOE43961.1"
                     /translation="MSANENKSSRSTLIVGAIGVVFGDIGTSPLYALKETFAGHHPIG
                     VEAASIMGVLSLIFWTIMALVTLKYVAIIMRADNRGEGGSLALLARVTELTKNSRATW
                     FVTMLGIFAAALFYGDSMITPAISVLSAVEGLEVVAPQFKDYVLPITVVVLSGLFWIQ
                     SKGTAVMGRLFGPVMCLWFAVLAVTGVISILRAPQVLLALNPMYAVEFLVSDPWRSFL
                     ALGAIVLSVTGGEALYTDMGHFGKYPIRLAWFTFVLPALVLNYYGQGALLLTDPGAIQ
                     NPFYRLTPDWALLPMVALATLATVIASQAVISGAFSVARQAVQLGYLPRMKIVHTSSM
                     EAGQIYVPFTNLTLYIAVMALVLGFRSSSNLAAAYGIAVTGTMMIDTVLVAFVAVLAW
                     RWSPWLAVPLLGALLLLDFAFFSANVIKLLQGGWFPVVVALASFTTLTTWRRGRSLLF
                     QEMGNLTMPLDQFIRSIEGAALKRVSSTAVYLTSRLEGAPSALLHNIKHNEVLHARNV
                     LATVLTAEVPYVAEPDRIEIIDIGNSFYRVFIRYGFMEQPDLPKALVACGELGLVFDM
                     EKTSFFLSREVVVPKLAPPMMMWRELFFILMLRNAQSATDFFRIPTNRVVELGTLVEI
                     "
BASE COUNT          630 a          736 c          733 g          482 t
ORIGIN      
        1 ggtcagcggc ccatcttgaa ccttgatgct caaaaactcc aggtgctcct catagttggc
       61 actgacgatg ctgtaagccc gcagtagcgg cttgccgcct tccagacgca ggccaatcat
      121 ggtgaagtgc ccgttagaaa agcgcagtgt gggatctcgt gtggtggtga agctgaaaag
      181 tcgatcggtc cagtgatgaa cgctcaaaac ggtttcattc aaaaatgcgc tcatgtagct
      241 tgccttgaat taagaacggt aaaaggggga gagtctaaaa cacaggtcgg gtttggcact
      301 gacaattcac aaaccgtggg aaatgcataa ggggaagaag aatcctgagt atgttggctg
      361 ttttgtgcaa ctgggtcgta aatgactgca agagaagttc acgagccatt caaaaaaacc
      421 gaacagcttg cttgatcttc ggcaggtgcg ctgttgcgac tgacgacatc agatctcgac
      481 cagcgtaccc agttcgacca cccgattggt cggaatacgg aagaaatcgg tggcgctttg
      541 cgcattgcgc agcatcagga tgaagaatag ttcgcgccac atcatcatcg gtggtgccag
      601 tttcggcacc actacttcgc ggctgaggaa aaacgaagtc ttttccatgt cgaacaccag
      661 acccagttca ccgcaggcca ccagtgcctt gggcaggtcg ggttgctcca tgaaaccata
      721 gcgtatgaaa acccggtaga aactgttgcc aatgtcgatg atctcgatcc ggtccggctc
      781 ggcaacgtag ggcacctcag cggtcagcac ggtggccagc acattgcggg catgcagcac
      841 ctcgttgtgc ttgatgttgt gcagcaacgc cgatggcgcg ccctcaagcc ggctggtcag
      901 atagacagca gtgctgctga cccgcttcag ggccgctccc tcgatggagc ggatgaactg
      961 gtcaagcggc atggtcaggt tacccatctc ctgaaacagc agactgcgac cgcgccgcca
     1021 ggtggtcaac gtggtgaaag atgccagcgc gacgacgacc gggaaccagc cgccttgcaa
     1081 cagcttgatg acgttggccg agaaaaaagc aaaatcaagc agtagcagtg caccaagcaa
     1141 gggcaccgcc agccacggac tccagcgcca cgccagtacc gcgacaaacg caaccagcac
     1201 cgtgtcgatc atcatcgtgc cggtcaccgc aataccgtag gctgctgcca gattgctgga
     1261 cgaccgaaac cccagtacca gcgccatgac cgcaatataa agtgtcaggt tggtaaaagg
     1321 cacgtaaatc tgcccggctt ccattgaaga cgtgtgcacg atcttcatcc gtggcaggta
     1381 gccaagctgg actgcctgcc gtgccaccga gaaggccccc gatatcaccg cctgcgaagc
     1441 gatgactgtc gccaaggtgg ccagcgccac catgggcagc agcgcccagt ctggagtcag
     1501 tcggtaaaac gggttctgga tcgcgcccgg atccgtcagc aacaaggcac cttgtccgta
     1561 atagttcagc accagtgccg gcaacacaaa agtgaaccat gcaaggcgaa tcgggtattt
     1621 gccaaaatgt cccatgtcgg tgtagagcgc ctcgcccccg gtgacagaca ggacaatcgc
     1681 gcccaatgca aggaaactgc gccacggatc gctgaccagg aattcgacgg catacatcgg
     1741 gttcagggcc agcaacacct gtggagcccg caagatgctg atcaccccgg tgaccgccag
     1801 cacggcaaac cacaaacaca tgactggccc gaacagtcgg cccatgacag ccgtgccttt
     1861 gctctgaatc cagaacaggc ctgacagcac cacgacggta atcggcagca cgtagtcctt
     1921 gaactgcggt gccaccactt caagcccttc gacggcgctg agcacggaga tcgccggggt
     1981 gatcatgctg tcgccgtaga acagcgcagc ggcaaagatg ccaagcatgg tgacgaacca
     2041 ggtggcacgc gaatttttgg tcaattccgt gacgcgggcc agtagcgcca ggctaccgcc
     2101 ttcgccgcgg ttgtcggcgc gcatgatgat ggccacgtat ttcagcgtga ccagcgccat
     2161 gatggtccag aaaatcagcg acaaaacccc catgatgctt gccgcctcca ccccaattgg
     2221 gtgatgcccg gcaaaggttt ccttgagcgc ataaagcggg ctggttccga tgtcgccaaa
     2281 aacgacaccg atggcaccga cgatcagcgt tgaccggctg ctcttgtttt catttgcgga
     2341 catggtgggg taattccggt ttttggtggg tctgattctg actgcttcaa cgtggactac
     2401 tcaattggac gcgctcaact gggccgctgg cggcgcatct gggtaggcgc aacgacggca
     2461 ccgacccctg tccattggaa gcaacaaccc caagcgcgtt gccagtggtg caaatgggtg
     2521 cgtagcgttt ccacccgata gttgaacccc aagaaagcta aaaaagtaaa tatgcatgag
     2581 g
//
LOCUS       DAOSLL010000651         2557 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_35173_length_2557_cov_4.223421, whole genome shotgun sequence.
ACCESSION   DAOSLL010000651 DAOSLL010000000
VERSION     DAOSLL010000651.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35173_length_2557_cov_4.223421"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..138)
                     /locus_tag="PLB25_20375"
     tRNA            complement(63..138)
                     /locus_tag="PLB25_20375"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(103..105),aa:His,seq:gtg)
     gene            complement(534..1226)
                     /locus_tag="PLB25_20380"
     CDS             complement(534..1226)
                     /locus_tag="PLB25_20380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HOE43962.1"
                     /translation="MQKPLMHHLVKPLIYFKRLCYLFVLIAFSSAYAGSYEDFFSAVK
                     FDDERGLLALLQRGFDANTVGPTGLPALLQALKERSPKVVRVLIEWPKTRVEVRNAVD
                     ESPLMLTCLTGDLELSRLLIRKNADVNKPGWTPLHYAATNGHVPVIRLLLDENAYIDA
                     ASPNGTTPLMMAAHYGSSEAVKLLLESGADPVLKNIQGLSALDFANRAGNEDSATMIQ
                     KFIRDMQPKGTW"
     gene            complement(1211..2017)
                     /locus_tag="PLB25_20385"
     CDS             complement(1211..2017)
                     /locus_tag="PLB25_20385"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013802700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HOE43963.1"
                     /translation="MFTDSHCHLDFPELMHELPAILKAMANAQVNRALCISTTLEEFP
                     AVHQLALSHPNLWATAGVHPDSEGVREPGLADLLALGSLPRVLGIGETGLDYYRLNGR
                     NVADMEWQRNRFRTHIMAARQLAKPLVIHTRSAAMDTLAILREAGEDGSAGSAGGVFH
                     CFTEDAATARTALDLGFYISFSGILTFKSAQTIRDVATFVPLDRLLIETDSPYLAPVP
                     HRGKTNNPSFVPLVAAQLAQLRQMPIQQIARLTSDNFDTLFKGVCACKSR"
     gene            complement(2057..2428)
                     /locus_tag="PLB25_20390"
     CDS             complement(2057..2428)
                     /locus_tag="PLB25_20390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HOE43964.1"
                     /translation="MKTTPSSSQSVPRPSVIQFPIPDLSTLAAAYMPLLIDGGIFVQT
                     NRDYQLGDSVYALISLPDDPQRYPVAAKVVWVTPPKAAGGRVQGVGIRFPKDEKSLAL
                     KAKIEAALSAYVGTDRATQTI"
BASE COUNT          589 a          676 c          707 g          585 t
ORIGIN      
        1 ttaatattta agcagtaaga ggtggcgttt tggtggggtt tccgttctgg cactatgtag
       61 cttggggtgg ctgatggggc tcgaacccac gacaacagga atcacaatcc tggactctac
      121 caactgagct acagccaccg ttggcgcaga gtataaccgg aggatggcaa gcctacacac
      181 caaagttcct ctttgcgcgc gaggctgacc gcaggtcgcg gacgacttca ctacctctga
      241 tttggttccc actgcgcgcg aggctgaccg gttgacaaat tcttcgttcg gtcggcgata
      301 aggccatttg ttagcacgct cgatctcggc tagatgcgac acgcgagtta gaagtttctt
      361 ggagcgaggc cagctgttct tgatggaacg gtattgctga agcaaattcc tgattcccat
      421 ccaacaggaa gtaggggctg gcagacaaac caccgtgcca gcggcttggt tgaccaagct
      481 tttcttgatc tcttacttta cataatatac atcgtataaa gtcaaatcaa tctctaccag
      541 gttccttttg gctgcatgtc ccggatgaat ttctggatca tggtggcgga atcctcgttg
      601 ccagcgcgat tggcaaagtc cagtgcggac aaaccctgga tattcttcag cactggatca
      661 gcgccggatt cgagcagcag cttgacggct tcactcgagc cgtaatgcgc ggccatcatc
      721 aatggtgtcg tcccgttggg tgatgctgcg tcgatatagg cgttctcgtc gagcagcaga
      781 cggatcaccg gtacatgacc gttggtcgcg gcgtaatgca gcggggtcca gccgggcttg
      841 ttgacatctg cattttttcg gatcaacaaa cggctcagtt caagatcgcc ggtcaggcaa
      901 gtcagcatca agggactttc gtcaactgcg ttgcgcactt caacccgggt tttgggccat
      961 tcaatcaaca cccggaccac cttgggtgac cgttccttca atgcttgcag cagcgccggg
     1021 aggcctgtgg ggccgactgt attggcatca aaaccacgct gcagcagtgc caaaagccct
     1081 cgttcatcgt caaactttac ggcgctgaaa aaatcttcat acgacccggc ataggctgaa
     1141 gaaaatgcta ttaaaacaaa tagatagcag agtcttttaa agtaaatcag aggttttacc
     1201 agatgatgca ttaacggctt ttgcacgcgc aaactccttt gaacaaggtg tcaaaattgt
     1261 cactggtaag ccgtgcgatt tgttgaatcg gcatttgccg gagttgcgcc agttgcgccg
     1321 ccactaatgg cacaaacgaa gggttattgg ttttacctcg gtgtgggact ggtgccaggt
     1381 aggggctgtc ggtttcgatc aacagccggt cgagcggcac aaaagtcgcc acgtcgcgaa
     1441 tcgtttgcgc acttttgaag gtcaaaatac cagaaaaaga aatataaaaa cccaggtcca
     1501 aggcggtgcg ggcggtggct gcatcttcgg taaagcagtg aaaaacaccg ccagcacttc
     1561 ccgccgagcc gtcttcaccc gcttcgcgca aaatagccag cgtatccatg gccgcactgc
     1621 gggtgtggat caccaagggt tttgccaatt gccgggccgc catgatgtgg gtgcgaaacc
     1681 ggttgcgctg ccattccatg tcggccacgt tgcgaccatt gagccggtag tagtcaagcc
     1741 cggtttcgcc gatacccagc acccgtggca gactgcccag tgccagcaag tccgccagac
     1801 cgggttccct gacaccttcg ctatccgggt gaacaccggc tgttgcccac aaattggggt
     1861 gtgacagagc caattgatgt actgcgggga actcttccag tgtggtgctg atgcacaagg
     1921 cgcgattcac ctgggcattt gccatagcct tcaaaatggc tggcaactcg tgcatcagtt
     1981 cggggaaatc aaggtggcag tgagaatctg taaacatgca aggccaaaaa agcgctgaag
     2041 accaacagcg gttgactcaa atggtttgtg tggcccggtc agtgccgacg taagcgctca
     2101 aagctgcttc aatttttgct ttgagcgcca acgacttttc gtccttggga aacctgatgc
     2161 cgacaccttg aactctgcca cctgctgcct tgggcggggt cacccatacc accttggcgg
     2221 ctaccgggta acgctgagga tcatccggca gcgaaatcag cgcataaacc gaatccccaa
     2281 gttgatagtc acgattggtc tggacaaaga taccgccatc gatcagcagc ggcatataag
     2341 cggccgccag ggttgacagg tccgggatgg gaaactgaat cacgctgggg cgcggcacgc
     2401 tctgactgct ggaaggggtg gtcttcatga tttcaagttt agtgccaatt gcgcctgact
     2461 gaccagggcc tcttgcatca ggcccacgtt aaaagggtgc tccaccatac gccgggtgag
     2521 actcagcgct tttgaccagt ttgtcaggct ttgccat
//
LOCUS       DAOSLL010000652         2531 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_35629_length_2531_cov_3.760097, whole genome shotgun sequence.
ACCESSION   DAOSLL010000652 DAOSLL010000000
VERSION     DAOSLL010000652.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35629_length_2531_cov_3.760097"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(592..2340)
                     /locus_tag="PLB25_20395"
     CDS             complement(592..2340)
                     /locus_tag="PLB25_20395"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid-cluster NAD(P)-dependent oxidoreductase"
                     /protein_id="HOE43965.1"
                     /translation="MTTPNKGLESTPLVETSANLLFALFIFIADAEGNMSAREVQNLQ
                     EMIEDASWCGDADIKMGLENLRVRYPNLWKDYQQKSFAHDLKSILVRIQTVGAGVADP
                     AGALDSLRTFVNRLCQNPSPTLVRLGLSSLRAAKQKAQKDALRALSMPDSANADSAIG
                     AEGSFAPVFMPAEASQPNVPIGPELDRAIWPSATLKLATENLWKRGKTPVVCVAVIPE
                     THDVKTFVFQAVQPVLFVYQPGQFATLELPIEGKTVRRSYTISSSPSRPHALSVTVKR
                     VPGGLVSNWLHDNMKVGFQFPLLGPHGEFTCFNAPAEKLLLIAAGSGVTPIMSMLRWL
                     ADTCSTADIVFINNIRTPADIIFRNELKYLGMRLGGTLKMGLIPSSVEAGQAWNGPVC
                     RFSKHLLQLWAPDFVEREVFVCGPSGYMDAVRTTLKQIGFPADRYHQESFGGPAPAQK
                     VVNATTEAIAAIVAPAPAPAPAAKAASVRVEAPVVQAPVASKAEANIELVFSISSKTV
                     KVTSGDFILDVAEEHGIVLPSSCRAGNCGTCKVKKTEGNVEMDNQQALGEADLSEGFV
                     LACVGRACSRRVVLEA"
BASE COUNT          583 a          708 c          668 g          572 t
ORIGIN      
        1 aaaccaaaga gcgtggaaaa attgcgtggt atggcggggt tgccaatgat gccgtttgaa
       61 gtgaacggtg gaacttgctc gggtgaggca ccggtgccaa cctgtacacg cactcgaccc
      121 ggtccaaagt gatcaggact gttaccgtcc actccaatgg gtatcgcaac gttgtcgcca
      181 aatccgaaaa ctggatggtg gcaggtaacg cacgcaactt ggcgatcaaa gctcatgtct
      241 ttggaaaaaa acagcagttt ggccagttca acgcaagctt tgagtgccgg ctcagtttca
      301 ccggatatcg ctgggattct tggcgcgcct ggcaacccgc aaagtgactc agtggcatca
      361 cccttgagtt tggcggctgc tatcagagcg cattatttcc ggtctgttgg cagttctggt
      421 gtcggcgaac tgccaccgcc acccgggcac ccggccaaaa gaccagctat cgcgatcggt
      481 atcgccatta ctcttacgct gcttttcgtg caagacctcg aagtcatcaa gaaaccatct
      541 ccaaattgcc agccgggcaa tgcggttttt tgcgctatgc ccgggcgacc gctacgcctc
      601 aagaacaacc cgacgactgc acgcccgacc cacacaggca agaacaaagc cttcactcaa
      661 atcggcttcg ccaagagcct gttgattgtc catttcaaca ttgccctctg tctttttgac
      721 tttgcaggta ccgcagttgc ctgccctgca ggaacttggc agcacgatgc cgtgctcttc
      781 ggctacatcg agaatgaagt cgcctgatgt gaccttgacc gtttttgaac tgatcgagaa
      841 aaccagttca atatttgcct cggccttgct ggccacaggc gcttgcacga cgggcgcttc
      901 aacccggact gatgccgcct tggcggcagg tgcaggtgca ggtgcaggtg caactatcgc
      961 tgcaattgcc tcggtggtcg cattcacgac tttttgggct ggtgctggcc cgccaaaact
     1021 ctcctggtga taccggtcag ccggaaaacc gatttgcttc agtgttgtgc gaacggcatc
     1081 catgtaccct gaaggcccgc agacaaaaac ctcgcgctcc acaaagtcgg gggcccacaa
     1141 ctggagcaaa tgtttgctga atcgacaaac cgggccgttc caggcctgcc cggcttcaac
     1201 gctgctggga atcagcccca tcttgagtgt gccgccaagg cgcattccca agtatttgag
     1261 ctcgtttcta aatatgatgt cggctggtgt tcggatgttg ttaatgaaca cgatgtcagc
     1321 cgttgaacac gtatcagcca gccagcgcaa catcgacatg atgggcgtca ccccacttcc
     1381 agcagcgata agcaacagct tttctgcggg tgcgttgaaa caagtgaact caccatgcgg
     1441 gcccaagagc gggaactgaa aacccacttt catgttgtca tgcagccagt tcgaaacaag
     1501 gccacctgga acgcgcttca cggtgaccga caaggcatgc ggccgggatg gcgacgatga
     1561 aatggtgtag ctgcgccgca ctgtcttacc ctcaatcggc agttccaggg ttgcgaactg
     1621 tccgggctga tagacaaaaa gaacgggttg cactgcctga aaaacaaaag tcttgacatc
     1681 atgggtttct ggaatgacgg ctacgcagac caccggtgtt tttccacgtt tccatagatt
     1741 ctctgtggcc agcttcagcg ttgcactcgg ccaaatagcg cgatcaagct ctgggccaat
     1801 gggaacattc ggttgactcg cctcggctgg cataaacact ggcgcaaatg aaccctctgc
     1861 accaatcgcg ctgtctgcat tggcactatc tggcatggac agcgcgcgca acgcgtcctt
     1921 ttgagccttt tgcttggcgg cacgaagcga actaagtcct aatcgaacga gtgtcggcga
     1981 aggattctgg caaaggcgat tgacaaaagt tcgcagcgaa tccagcgccc cagcgggatc
     2041 tgcaacccca gcgcccacgg tctgaattct caccaagatg cttttcaggt catgcgcaaa
     2101 tgacttttgc tggtaatcct tccagaggtt ggggtagcga acacgcaggt tttccagccc
     2161 cattttgatg tcagcgtctc cacaccaact ggcatcttcg atcatctcct gcagattctg
     2221 aacttctcgg gcgctcatgt tgccttcagc atctgcaata aagatgaaca gggcaaagag
     2281 caaattggct gatgtttcaa ccaatggcgt tgattcgaga cctttgtttg gcgtagtcat
     2341 gatttcattt ggtccgttgt caagaaaaat cagcttcgga cataacccgc gaccacggtc
     2401 aacatgctca tcgaaaccgt tgaatttcgc tccatcaacc ggcgcagtct ttctccattg
     2461 atggtgagta cgcgcgcctt gggcgacgaa atgcgggtag aggcgaccct ggggtgtcca
     2521 gtaatgacac c
//
LOCUS       DAOSLL010000653         2530 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_35652_length_2530_cov_2.346667, whole genome shotgun sequence.
ACCESSION   DAOSLL010000653 DAOSLL010000000
VERSION     DAOSLL010000653.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35652_length_2530_cov_2.346667"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1708
                     /locus_tag="PLB25_20400"
     CDS             <1..1708
                     /locus_tag="PLB25_20400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018007794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HOE43966.1"
                     /translation="GFILIDRINRMNNLWYCETIEATNPCAEQPLPDYGCCCLGSIDL
                     TKHVRDAFTEDAQFDFDSFAGVVHTAVRMLDNVLDATFWPLEKQKIEASNKRRIGLGF
                     LGLGSALVMMGIKYNSEQGCAMAAKIAEMMRNEAYRASIKLASEKGSFPLLEAEKYLQ
                     SGFAKRLPADIREGIKTVGLRNSHLLSIAPTGTITLAFADNASNGIEPAFSWVYNRKK
                     RLADGGHRFYEVADHAWRLYRHMGGDVSTLPEYFVTALEMTAQEHMQMMEAVQPFIDT
                     AISKTVNVSADYPYDDFKGLYLEGWKAGLKGLATYRPNSVLGSVLSVGPTVTSPAVSP
                     DPHVANDDPLHKQFNARVDGELESVTTKIQMWTHEGKRSVYVTTSFMTVDGVVDGKAV
                     TIERPVEFFLAGSQRDAGQQWVDLSLRMLSMAARSGVSVAKTIENMREVAWDKGPVRY
                     GYATKADGTQVPRFHDSEVAAIGYALQEMLVRRGFLTSEGQQVPIAKLSKLLEARLSS
                     GRNAEAAGAPSVDASPHALRAGDHFAAGTGKKCGHCGAHDVHRIDGCTQCVSCGTVGS
                     CG"
     gene            complement(2069..>2530)
                     /locus_tag="PLB25_20405"
     CDS             complement(2069..>2530)
                     /locus_tag="PLB25_20405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43967.1"
                     /translation="MTRSFLRTFLCSFWMLWLPFSSQARVPEICVSTIAELKVLLADP
                     PLSLRWEETTMDDAKPLVVSIAERNGALSVEFVKATKGVWADISGVICKSDKAMEIRF
                     TAEQIHFGPAASWVLRYALSNGGKFTLTRLGEKQVQVQTTGWSGDFVAIEK"
BASE COUNT          569 a          673 c          701 g          587 t
ORIGIN      
        1 ggggttcatt ctgatcgatc gcatcaatcg tatgaacaac ctgtggtact gcgagacgat
       61 tgaagctacc aatccttgtg ctgagcaacc tcttccagat tacggctgtt gttgcctggg
      121 ctccattgac ctgaccaagc acgtacgcga tgcatttact gaagacgcgc aattcgactt
      181 tgattcgttt gctggagttg tgcataccgc tgttcgcatg ctcgataacg tgctggacgc
      241 caccttctgg cctcttgaaa agcaaaaaat tgaagcctca aacaagcgcc gtattggcct
      301 gggcttcctt ggtctgggca gcgccttggt aatgatgggc atcaagtaca actctgaaca
      361 gggttgcgcg atggctgcaa agattgcaga aatgatgcgc aatgaggcgt accgggcttc
      421 aatcaagttg gcttccgaaa agggatcgtt tccgctgctt gaagccgaaa agtatctgca
      481 aagcggcttt gccaagcgtc tgcccgcaga cattcgtgaa ggcatcaaga cggtgggtct
      541 gcgcaatagc cacctgctgt cgattgctcc tactggcacc attacactgg ctttcgctga
      601 caacgcatcc aatggtatcg agcccgcatt tagctgggtc tataaccgca agaagagatt
      661 ggccgatggc gggcaccgtt tttatgaagt cgctgaccat gcatggcgac tgtatcgcca
      721 catgggtggt gacgtgagca ctctgcctga gtatttcgtc acggcgctgg agatgacagc
      781 gcaggaacac atgcagatga tggaggccgt gcaaccgttc attgacactg cgatttctaa
      841 gaccgtgaac gtctctgctg actatcctta tgatgacttt aagggtttgt atctggaagg
      901 ctggaaggcc ggtctcaagg ggcttgccac ataccgtccg aactcggtgc tgggctcggt
      961 gctgtcggtt ggtccgaccg ttacttcacc tgctgttagc ccggatcctc atgtcgcgaa
     1021 tgacgacccg ctgcacaagc agtttaacgc ccgtgtcgat ggtgaactcg aaagcgtcac
     1081 caccaagatt caaatgtgga cccatgaggg caagcgctcc gtgtatgtca ccaccagttt
     1141 catgaccgtg gatggcgtgg tcgatggcaa ggcagtgacc atcgagcgtc ctgttgaatt
     1201 cttcttggcg ggcagtcagc gcgatgctgg acagcagtgg gttgacctga gcttgcgtat
     1261 gctctccatg gcggctcgct ctggcgtctc ggttgccaag actatcgaga acatgcgtga
     1321 agttgcctgg gacaaggggc cggtgcgtta tggttacgcc accaaggccg atggcaccca
     1381 ggttccgcgt tttcatgact ctgaagtcgc tgccattggc tacgctttgc aagaaatgtt
     1441 ggtgcgtcgt ggcttcttga cgtcagaagg tcaacaggtg ccaattgcca agttgtccaa
     1501 gttgttggag gctcgcctat cgagtggccg caacgctgaa gccgctggcg ccccgtcagt
     1561 tgatgctagc cctcatgcgc tgcgagcagg ggaccatttt gctgccggca ctgggaagaa
     1621 gtgcggccac tgcggtgctc acgatgtgca tcgcatcgat ggatgcactc agtgcgtgag
     1681 ttgcggaacg gtgggcagct gcggttagct caagccggtg gcgtagccaa aagtcgagga
     1741 atcaatgcgc cgcgtcacgg tcaagctgaa catgagagca atacactgct gcgttctgta
     1801 tcggcatacg ggtccacatc ggtgatgagg tggacctgca gggaactggt atgcaccaac
     1861 cgttgcaggg ccgaatccag aaaaggcatc tgattcagcg caccaaagac aaactgcatc
     1921 gtttcctgat ctgatactac agtgccgcac tcccaacgtg cggggggcgg ggggtaacct
     1981 ccgtccctac cgcaaccaaa caatgtgaat gcaaccagca atttcggctt tggcctatga
     2041 gttcaccaaa gacttacgac ttttgtggct acttctcgat tgcaacaaaa tccccactcc
     2101 atcccgtggt ttgcacttgc acctgctttt cgcccaatct agtcagagta aatttgccgc
     2161 cattactcaa ggcatagcga agcacccagc ttgcggcggg gccaaagtga atctgctctg
     2221 ccgtaaatct tatttccatg gctttgtcgg acttacaaat cacgccggaa atgtctgccc
     2281 aaacgccttt ggtggctttc acgaactcga cagaaagcgc tccgtttctc tcagcaatag
     2341 acaccaccag cggtttggca tcatccatgg ttgtctcttc ccatctcaac gatagcggcg
     2401 gatcggcaag caatactttc aactccgcga tcgtggagac gcatatttct ggaacgcggg
     2461 cctgactact aaagggcagc caaagcatcc agaaagaaca caggaaagtt cgcagaaacg
     2521 agcgagtcat
//
LOCUS       DAOSLL010000654         2527 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_35697_length_2527_cov_3.988673, whole genome shotgun sequence.
ACCESSION   DAOSLL010000654 DAOSLL010000000
VERSION     DAOSLL010000654.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35697_length_2527_cov_3.988673"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(162..668)
                     /locus_tag="PLB25_20410"
     CDS             complement(162..668)
                     /locus_tag="PLB25_20410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012418827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOE43968.1"
                     /translation="MLVWVAPGAAQSPAAPVPPVASVASVDLARYAGKWFEIGSFPMY
                     FQRNCVADTTAEYTLTPQGKVQVTNRCRTDSGFDEAIGSATAVEGFDNSRLKVSFFWP
                     FKADYWVIGLDADYRWAVVGNPNRKYLWLLSRTPQLPPALLEAAMAAASAQGFDLSQL
                     RYTRQTAP"
     gene            866..1489
                     /locus_tag="PLB25_20415"
     CDS             866..1489
                     /locus_tag="PLB25_20415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HOE43969.1"
                     /translation="MTYPAPTINIDSVRQQARKNIELGAVTSSYTADRPVVLKMLNES
                     LATEIVCVLRYRRHHFMARGIQAQSVASEFLVHSNEELGHADLLATRILQLGGEPDFS
                     PETLLARSHAEYIEGKSLGDMITENLIAERIAIDSYRNFISYLRDQDPTTRRMLEGIL
                     ATEEEHADDLADILLKLPATPEAAAPADAGSAVKVAAPIKLIKKDAS"
     gene            1486..1644
                     /locus_tag="PLB25_20420"
     CDS             1486..1644
                     /locus_tag="PLB25_20420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005801544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HOE43970.1"
                     /translation="MSLGLILLIVLILLLIGAIPSWPHSRSWGYGPSGGLGLLVIIIL
                     VLVVMGRI"
     gene            complement(1922..2218)
                     /locus_tag="PLB25_20425"
     CDS             complement(1922..2218)
                     /locus_tag="PLB25_20425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43971.1"
                     /translation="MELLDSQWQYPSMPWHREPSHGRNILKKIGLCLRGNHAIISVRS
                     CSAGNWKTPLWMGLAILCSHGKGFVSRRFWFNKEGCWQASGRLAQCSGLYVKFA"
     gene            2235..>2527
                     /locus_tag="PLB25_20430"
     CDS             2235..>2527
                     /locus_tag="PLB25_20430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005796751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HOE43972.1"
                     /translation="MKPIKTFLWAFLLLLTALWWMTDTPAMAGLGDFSAWRSVLMQYS
                     GVLAIGVMSVAMLLAVRPVLFEPYLGGLDKMYRLHKWLGISALVISVSHWLLS"
BASE COUNT          540 a          746 c          719 g          522 t
ORIGIN      
        1 accaccgatc tggtggctca ctttgagcag cgcaacaccg cccagagcgg caaggcgatg
       61 gtggtgatgg ttgatcgcag gttgcgctac tggtgctaat gcgattgaac caacaattcg
      121 ccagcttcag cacagcgagt gccagcagct ttcaaaagcg gctatggcgc agtttgtcgg
      181 gtgtagcgca actggctcag gtcaaaccct tgcgcgctgg cggctgccat cgcggcctca
      241 agcagggccg gtggcaactg cggcgtgcgc gacagcagcc acaggtattt gcggttcggg
      301 ttgcccacca ccgcccagcg gtaatcggca tctagcccga tcacccaata gtcggccttg
      361 aacggccaga aaaatgacac tttgaggcgg ctgttgtcaa acccctcaac tgccgtggcg
      421 ctgccaatgg cttcatcaaa accgctgtcg gtgcggcagc ggttggtgac ttgcaccttg
      481 ccctgcgggg tcaaggtgta ctcggccgtg gtgtccgcta cgcagttgcg ctggaaatac
      541 atcggaaaac tgccaatctc gaaccacttg ccggcgtagc gtgccaggtc aaccgatgcc
      601 acgctggcca ccggcggcac tggggctgca ggactttgcg ctgcgcctgg tgcaacccag
      661 accagcatga caccggccat gaaaaatgct ttgaaaacca tgttgaaact cctgtgaacg
      721 gcgaaagtgc ctgaaaggcc tgaaagcccc gaaagcagcc atgttggcac acctgcctgt
      781 tgccacttgg ttcgccatcg aacagacccc ggcaggttga caccacatca tcgcttacct
      841 acttactcaa ccaccaaagg cagccatgac atatcccgcc cccaccatca acattgattc
      901 cgtgcgccag caggcccgca aaaacatcga actaggtgcc gtcacctcca gttacacggc
      961 agaccgaccc gttgtgctga aaatgctcaa tgaatcactg gccaccgaga tcgtttgtgt
     1021 gctgcgttac cggcgccacc acttcatggc gcgcggcatt caggcgcagt cggttgccag
     1081 cgaatttctg gtgcactcca acgaagaact gggacacgcc gacctgcttg ccacgcgcat
     1141 cctgcagctc ggcggcgagc cggatttttc gcctgaaaca ctgctggctc gcagccacgc
     1201 cgagtacatc gaaggcaaaa gcttgggcga catgatcacc gaaaacctga tcgccgagcg
     1261 cattgcgatc gacagctacc gcaatttcat cagttatctg agagaccagg acccgaccac
     1321 ccggcgcatg ctcgaaggca ttctggccac cgaagaggag cacgcagatg acctggccga
     1381 catcttgctg aaactgccag caacgccaga agccgccgcg ccagccgacg cgggcagtgc
     1441 ggtcaaggtc gcagcaccca tcaaactgat caagaaggac gcatcatgag cctaggcctt
     1501 attctgctga ttgtgctgat tctgttgctc attggcgcga tcccaagctg gccgcacagc
     1561 cgaagctggg gctacgggcc gagcggcggg cttgggctgc tggtgatcat catcctggtt
     1621 ctggtggtca tggggcgaat ctgacccttg tcccttggcc gccgcaatga ccctaagccg
     1681 gacgggccgg aattggacgg ccttcgtcgc gagggcgcga ttcctgcaaa ttcaaatagg
     1741 ctccgactgg ttgtgtagga gtcgcgccct cgcgacgaaa gctcatcgat tccggctcgt
     1801 ccggcttagg ttgaagccgc atggctggcc tgctgctgac ggtccgggtt tggccgatgc
     1861 tgccaggcat gtccggataa ctatcaaata aatagcttgt atcgcttatt gcataagggg
     1921 ttcaagcgaa tttaacataa agaccagagc attgcgccag cctgcctgat gcctgccaac
     1981 agccttcttt gttaaaccaa aaccgacgag acacaaaacc tttaccgtgg ctgcatagga
     2041 tggccagacc catccataac ggcgttttcc aattccccgc tgagcaactc ctgactgaaa
     2101 tgatggcgtg attgccgcgc aggcaaaggc cgattttttt caatatgttg cgaccgtggc
     2161 tgggttcccg gtgccacggc attgatgggt attgccattg tgaatcgaga agttccacca
     2221 aaaaccgact caaaatgaaa ccaatcaaaa ctttcctctg ggcctttctg ctcctcctga
     2281 cggcgttgtg gtggatgacc gataccccgg cgatggccgg attgggcgac ttttctgctt
     2341 ggcgcagtgt gctgatgcag tacagcggcg tgttggccat tggtgtcatg agtgtggcga
     2401 tgctgctggc ggtgcgcccg gtgctgtttg agccttatct gggtgggctc gacaagatgt
     2461 atcggctgca caaatggctg ggcatttcgg cgttggtgat atcggtcagc cactggctgc
     2521 tttccaa
//
LOCUS       DAOSLL010000655         2524 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_35756_length_2524_cov_5.300527, whole genome shotgun sequence.
ACCESSION   DAOSLL010000655 DAOSLL010000000
VERSION     DAOSLL010000655.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2524
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35756_length_2524_cov_5.300527"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /gene="recR"
                     /locus_tag="PLB25_20435"
     CDS             complement(<1..588)
                     /gene="recR"
                     /locus_tag="PLB25_20435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HOE43973.1"
                     /translation="MSDTNALEALIQALRRLPGVGVKSAQRMAFHLLQHDRAGAQTLS
                     RALAEASAQIVHCERCHTFTEHSVCTTCLDVRRDAGKLCVVETPADQAAVERTGAYKG
                     LYFVLMGKLSPLDGIGPKDIGLKKLFDRALDGVVQEVILATNFTAEGEATAHVINEGL
                     KTRGVQLTRLARGVPVGSELEYVDLGTIAHALVDRR"
     gene            complement(770..1102)
                     /locus_tag="PLB25_20440"
     CDS             complement(770..1102)
                     /locus_tag="PLB25_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020229495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HOE43974.1"
                     /translation="MFNKGQLAGLMKQAQAMQDNMKKAQDELGSIEVSGESGAGLVKV
                     TMTCKHEVRRITIDPTLLAEDKDMLEDLVAAAFNAALRKAEDLSAEKMGKITAGIPGL
                     PSGMKFPL"
     gene            complement(1141..>2524)
                     /locus_tag="PLB25_20445"
     CDS             complement(1141..>2524)
                     /locus_tag="PLB25_20445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau C-terminal
                     domain-containing protein"
                     /protein_id="HOE43975.1"
                     /translation="PQKVPVTVLSRCLQFNLRPMAPETIREHLQTVLQTEQVVADQQS
                     LRLLARAARGSMRDALSLTDQAIAFGSGSLEEATVRQMLGSVDRSYVFRLLDALAQGD
                     GKTVVETSELLRQYGLSAASTLEEMSTLLQRMAVLQAVPDAPPGDEPDAELAELSRLA
                     GVMPPDETQLLYSICLHGRGELGLAPDEYAALTMVLLRLLAFKPTGAKAAQAEKKTLK
                     KAVPAADPPAARISVSNQAPALVDKASVASENVAIQALPASFEPIAQQPLPAGQHLPV
                     VTMPGATDHPAFQVLNQPLAHTDKAQAATTIEAIQVRVQADSMRETLAVGTSQALRPS
                     TEGDFWHATVQTLIAAEAISAMVRELALQSQLVARDTDQWLLRVERESLNQSGSRDRL
                     AAALAAAGHPVRLAIEVGRVTDSPARRNAAALNERQLMAEKIVFDDPFVQSMMREFGA
                     KIVPGSIKPV"
BASE COUNT          461 a          786 c          743 g          534 t
ORIGIN      
        1 acgccggtcc accagcgcgt gggcgatggt gcccagatca acgtactcca gctcactgcc
       61 aactggcacg ccacgggcca gccgcgtcag ttgaacacct ctggttttga ggccttcgtt
      121 gatcacatgc gcagtggcct cgccttcggc ggtgaagttg gtggccagaa tcacctcttg
      181 caccacgcca tcgagcgccc ggtcaaacag tttcttaagg ccaatgtcct tcggaccaat
      241 gccgtcgagc gggcttagct tgcccatcag cacaaaatac aggcccttgt aggcaccggt
      301 tcgctccacc gcagcctgat ctgccggggt ttcgaccaca cacagcttgc cggcatcacg
      361 gcgcacatcc agacaggtgg tgcagacgct gtgctcggta aaggtgtggc agcgttcgca
      421 atgcacaatc tgcgcgctcg cctcggccag cgcgcgcgac agggtttggg caccggcgcg
      481 gtcgtgctgc agcaaatgaa acgccatgcg ctgcgccgac ttgaccccca ctccgggcaa
      541 gcgccgcagc gcctgaatca gtgcctccag cgcattggtg tcagacatgt tgagcttttg
      601 cggggcgggc tggttggcat cggcctatgg cggtcatacc cacaggcccc tgatcagttg
      661 ggcaagagcc aggttcacgc aacaatactg cgatctttcc cacaagttcc tgatcagttg
      721 ggcaagagcc aggttcgctg cgcgtaacag cggtctttcc cacaaagaat cacaaaggaa
      781 atttcatgcc gctgggcaaa ccgggtatgc cggcagtgat cttgcccatt ttttcggcag
      841 agaggtcttc tgccttgcgc aaggcagcat tgaaagcagc cgccaccagg tcttccagca
      901 tgtccttgtc ttcggccagc aaggtcgggt cgatggtgat gcggcgcact tcgtgtttgc
      961 aggtcatggt gactttgacc aggccagcac cggactcgcc gctgacttcg atgctgccca
     1021 gttcgtcctg ggcctttttc atgttgtcct gcatcgcctg cgcctgcttc atcaggccag
     1081 cgagttgtcc tttgttaaac atgggtgaat tccttgggtt ggtttcgaat gggttttgaa
     1141 ttaaacaggc ttgatgctgc cgggcacgat tttcgcccca aattcgcgca tcatcgactg
     1201 tacaaagggg tcgtcaaaaa cgatcttctc ggccatcaat tgccgctcgt tcaatgctgc
     1261 cgcattgcgt cgggccgggc tgtctgtcac ccggccgacc tcgattgcca gccgcaccgg
     1321 gtggcccgct gccgctagcg ctgcggccag tcgatcacgg ctgccactct ggttgagcga
     1381 ttcgcgctcg acccgcagca gccactggtc ggtgtcgcgg gccaccagtt gcgactgtaa
     1441 tgccagctcg cgcaccatgg cgctgatggc ttcggccgcg atcaatgtct gaactgtggc
     1501 atgccaaaag tctccctcgg tgctgggccg cagcgcttgc gaagtgccaa ctgccagcgt
     1561 ttcacgcatc gagtcggcct gtacgcgcac ctgaattgct tcaatagttg tagctgcctg
     1621 cgctttatca gtatgggcta gaggctgatt tagcacttga aaagccggat ggtcggtggc
     1681 accgggcatg gtgaccacag gcaaatgctg accggctgga agtggctgtt gcgcgatcgg
     1741 ctcaaagctc gctggcaagg cctgaattgc tacattttca gaagctactg acgctttatc
     1801 tacaagggct ggtgcctgat ttgataccga aattcgggcg gctggcgggt cagccgcagg
     1861 gacggccttt ttcagtgttt ttttttccgc ctgggccgct ttggccccgg tcggtttgaa
     1921 tgccagcaga cgcaacagca ccatcgtcag cgccgcatac tcgtctggtg ccagaccgag
     1981 ttcaccgcga ccatgcaggc aaatgctgta gagcaactgg gtttcatcgg gcggcatcac
     2041 accggccagg cgactgagtt cggccagttc ggcatcgggc tcatcgcccg gcggcgcatc
     2101 cggcaccgcc tgcagcaccg ccatgcgctg cagcaatgtg ctcatttctt ccagcgtcga
     2161 ggccgcgctc agcccgtatt ggcgcagcag ttcagaggtt tccaccacgg tcttgccgtc
     2221 accttgcgcc agcgcatcaa gcagccgaaa cacataagac cggtcaacgc tgcccagcat
     2281 ctgacgcact gtggcctctt cgagcgagcc cgagccaaag gcaatcgcct ggtccgtcag
     2341 gctcagcgca tcgcgcatgg aacccctggc agctcgcgcc agcagccgca gcgactgctg
     2401 gtcggccacc acctgctcgg tttgcaacac tgtctgcagg tgttcacgaa tcgtctcggg
     2461 ggccatcggg cgcaggttga actgcaggca gcgcgacagc acggtgaccg gcaccttttg
     2521 cggg
//
LOCUS       DAOSLL010000656         2520 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_35835_length_2520_cov_4.116024, whole genome shotgun sequence.
ACCESSION   DAOSLL010000656 DAOSLL010000000
VERSION     DAOSLL010000656.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35835_length_2520_cov_4.116024"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="PLB25_20450"
     CDS             <1..602
                     /locus_tag="PLB25_20450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43976.1"
                     /translation="LDCMRNCNPIDPCTPLLPIIQAGHGMNCREHVDEVRVTGTPFCQ
                     VFLIQAGGRRANGQKLVEGDELAVLLRAHRVSGSKPVESAALDWFNNLHGHLLEAVAI
                     WRRLSRLHQQSHAFQNIAGRFSSPRPQWTMDACGAVLLVMLMLQVAHQSVRLDGFAAK
                     KFFYLTSVEVAVAVMLVQGIGVPAVVQTHRVLSKPCLSG"
     gene            complement(1016..2200)
                     /locus_tag="PLB25_20455"
     CDS             complement(1016..2200)
                     /locus_tag="PLB25_20455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HOE43977.1"
                     /translation="MKIFQHHTKHFLRVGLAAALLGTVALASAAPIYNSITPLGTLGG
                     RLNTVVSDFNDAGQMVGYFQTEDSRGNTVNHAFLYSAGVMTDLGTLGGKNSYADAINN
                     AGQVVGNAALEPVAPSFSQIPKAFLYGNNVMSNLGIPGDSSYAFDINDNGLVVGYSNT
                     RNLRHAFLYSNGITTDLGTLGGLESEANGINNAGTVIGESNTSTNLRHAFMYSDGKMT
                     DLGTLGGTLSTARDINNAGQVVGYSTISSRGPARAYLYSGGKMTDLGTLAAVNDPDRN
                     TYESFAYGINDAGQVVGQAQNSWFFSSPFLYSGGVMKNLNDLIDPASDFIISQAFDIN
                     ASGDILASGCLRNGTKCQSLLLESIAAPPNDVPEPETLALVSLGLAGLALGSRKRKHA
                     VA"
BASE COUNT          534 a          694 c          727 g          565 t
ORIGIN      
        1 atctggactg tatgcgcaat tgcaacccca ttgacccatg tacgccgctt ttgccgatta
       61 tccaggcggg ccacgggatg aattgccgcg agcacgttga cgaggtgcga gtcaccggca
      121 ccccgttttg tcaggtcttt ctgatacagg ctggtggtcg ccgggccaac ggccagaagc
      181 tggtagaagg cgacgaactg gccgtgctgc tgcgcgcgca ccgtgtttct ggcagcaagc
      241 cggtggaaag cgccgcgctc gactggttca acaacttgca tgggcatctg cttgaggcgg
      301 ttgccatctg gcgccggttg agcaggctgc accagcaaag ccacgcgttt caaaatatcg
      361 ctggcaggtt ctccagcccg cgcccgcagt ggaccatgga tgcctgtggc gcggtgctgc
      421 tggtcatgct gatgttgcag gttgcccacc agagcgtgcg ccttgatggc tttgcagcga
      481 agaaattttt ttacctcacc tcggtcgagg tcgccgtggc agtcatgctg gtgcaaggca
      541 ttggcgtgcc agccgttgtg cagacgcatc gggtgttgag taaaccatgc ttatccggtt
      601 agtcctcaag aggtttgaca ttctcggatg tgtgctttga cttctgccgg aagggcgcag
      661 gccacttggg aactcccgac atcgaaaggg tgagacaggt caaaacaccc cgataacggt
      721 gcgtgcattg ggtcttggga agcaaccgga taagcatgga gcaaagcttt gcgccgccag
      781 gaagttactt ggaaaagctg actttgaccc ctggagcgac ctggtgtgct gcttgctgga
      841 tctggggcgc atgcgctacg ccactaattt gctatttagt ttatagctac tgaggcaacc
      901 tggatgtgcg ttagagccag ttttgacatt ccttattgca atctacaccg tccggcagaa
      961 gtgaaattag gggggggcaa cagcccccga ccaacctcac tgagcaaaaa aacgttcagg
     1021 ccactgcatg tttacgcttg cgcgacccca gtgccagccc cgccagaccc aggctgacga
     1081 gggcgagggt ttcgggttcg ggcacatcgt tgggcggggc cgcaatgctt tccagcagca
     1141 aactctgaca cttagttcca tttcgcaggc agccggaggc caaaatgtcg cctgaggcat
     1201 tgatatcgaa tgcttggctg attatgaagt ccgatgctgg gtcgatcaaa tcgttcaaat
     1261 tcttcatcac gccgccgctg tacaaaaacg ggctgctaaa aaaccaagaa ttttgcgcct
     1321 gccccactac ctgcccggcg tcgttgatgc cataggcaaa gctttcatag gtattacgat
     1381 cagggtcgtt gaccgcggca agggtaccta ggtcagtcat cttgccgccg ctgtacaaat
     1441 aggcacgggc aggaccgcgg ctagatatag tcgaataccc aaccacctga ccggcattgt
     1501 tgatgtcgcg ggctgtgctg agagtgccac ccagggtacc caagtcggtc atcttgccgt
     1561 cgctgtacat aaaggcatgt cgaaggttgg tggaggtgtt cgattcccca atcaccgtcc
     1621 cggcgttgtt gatgccattg gcttcgcttt ccagaccacc caaggtgccc agatcggtcg
     1681 tgatgccgtt gctgtacaaa aaggcatgcc gcaagttacg tgtgttcgag taccccacta
     1741 caagcccgtt gtcattgatg tcaaaggcat aactgctgtc acccgggata cccaggttgc
     1801 tcatcacgtt gttgccatac aaaaaggctt tgggaatctg cgaaaaagac ggtgccaccg
     1861 gttcaagcgc cgcattcccc accacctgcc cggcgttgtt gatggcatcg gcgtagctgt
     1921 tcttgccgcc cagggtgccc aggtcggtca tgacaccggc gctgtacaaa aaggcatggt
     1981 tgaccgtgtt accacggctg tcttcggtct gaaaataccc caccatctgc cctgcatcgt
     2041 tgaaatcgga gacaacggtg tttaggcggc cgccgagggt gcccagcgga gtgatgctgt
     2101 tgtaaatggg cgcggcgctg gccagcgcca cagtgcctag caacgctgcg gccaggccga
     2161 cccgcaggaa gtgttttgta tggtgttgaa aaattttcat ggttcaggtc aaggtgtaag
     2221 ggattgaaaa acacggtccg gaatcagacg tggctgccat acagcatgat ctgtaccaat
     2281 ctgcgcattc gccctgccag accctgcatt tccctgctac agccgagatg gttggcttgc
     2341 gagttgctct gccctgacaa aaatggcgac tcattcaaat caatgagttc gggtcaccac
     2401 cacaattgcg gtgtcttggt ttgcactggc aaacgccagg tttgcatacc gaggcagcgt
     2461 atccggtcgc gccgaagcgg ccccaggcca ctgtcaaatt ctccgtctca tcaagattga
//
LOCUS       DAOSLL010000657         2512 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_35967_length_2512_cov_4.927554, whole genome shotgun sequence.
ACCESSION   DAOSLL010000657 DAOSLL010000000
VERSION     DAOSLL010000657.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35967_length_2512_cov_4.927554"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(74..1012)
                     /locus_tag="PLB25_20460"
     CDS             complement(74..1012)
                     /locus_tag="PLB25_20460"
                     /inference="COORDINATES: protein motif:HMM:NF012451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HOE43978.1"
                     /translation="MSHYDILEVSPRASAEVIRAAYKSLMQRHHPDRNADAIEATDRT
                     ALIAQAYAVLSDPQQRRGYDESLVKPPVVERSPQGGSRRTPVSRAGLAEKAGRSSAWR
                     AWYAPALILCIMAAGSAILFLSKENTAPAPTRQEPTPLSAHRLDATAPLAAPGQTAPW
                     PMIDMRPAKPDETPAQLQARTISAFVTDLSIALDATDPTQPGKAHVLHIPSLGLRVGT
                     GEPGRFTRRIQAQRSQIIEQLLVTLAKSPYHELINADGDLYLKRQIAQAVIAVVGLDK
                     APATAAVTPPLQGLAVPQEAPAAPLEVLLPLSFSVN"
     gene            complement(1042..1404)
                     /locus_tag="PLB25_20465"
     CDS             complement(1042..1404)
                     /locus_tag="PLB25_20465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43979.1"
                     /translation="MRRRSPGRFGKFSVLAVLVGLLLGACKGQVQPDAASFVGKWQSS
                     KLTTPLHLYDNGEWEIKNDNGGVLQYGLWAYQDSRIIWHFKSAGQIGREVNQVESVNA
                     REFRLREDGQITVFRRLD"
     gene            complement(1423..2352)
                     /locus_tag="PLB25_20470"
     CDS             complement(1423..2352)
                     /locus_tag="PLB25_20470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43980.1"
                     /translation="MTQKKSLYELLEVQQDADYADIRAAYERLQRLLESQQSTLSRED
                     YATQLRLLKVAFSALSAPSSRDAYDAGLHSRAESARAGSALQVTPAASSNTAAIRADA
                     LMMRAEAMTLRADALGLKADLLTGQLDGPGGAATGPAASRWFKTMKRALLVLGTLAAL
                     GMVFKLVFMLVLSPRPSDDSVGARSQTEDKVFLQEYYQTWGVRPASRAEAALMDAERR
                     KNDDAKTAQRLAEEEKKKAEQAERNFEAEARKRGEQVSIELQHAEKKAQQALLQEARL
                     KEEEARLSAEAERRRIEAERAKWQNVLRTPSSN"
BASE COUNT          453 a          793 c          760 g          506 t
ORIGIN      
        1 tgcttcatga gcaattcttc aagggaggca aattttcgat ggcgcggcgc agccggttgc
       61 aggccatggc gcgtcaattc accgaaaaag acaggggcaa caaaacctcc agcggtgctg
      121 ccggggcctc ctgcggcacc gccaaaccct gcagcggcgg tgtcacggct gcagtggcgg
      181 gggctttgtc aaggccgaca acggcgatca cggcctgggc gatctggcgc ttcaggtaca
      241 ggtcgccatc ggcgttgatc agttcgtgat agggggattt ggccagcgtg accaacaact
      301 gctcgatgat ttgcgagcgc tgcgcctgta tccggcgtgt gaatcgccct ggctcacccg
      361 tgcccacccg aagccccagg ctggggatgt gcaacacatg ggccttgccc ggctgcgtcg
      421 ggtcggtggc atcaagtgcg atcgacaagt ctgtcacaaa cgcggaaatc gttcgggcct
      481 gcaattgggc tggtgtctcg tcgggcttgg cagggcgcat gtcgatcatg ggccagggtg
      541 ccgtctgtcc cggcgctgcc aatggcgcgg tggcgtcaag gcggtgggcc gacagcggcg
      601 tgggctcttg ccgggttggt gcgggggcgg tgttctcttt ggacagaaaa agtatggccg
      661 acccggccgc catgatgcac aggatcagcg ccggggcgta ccaggcccgc caggcgcttg
      721 atcggccagc tttttctgcc agcccggcac gggaaaccgg tgtgcgccgg gagccgcctt
      781 gcgggctgcg ttctaccacc gggggtttga ccagcgactc gtcgtaaccg cgccgctgct
      841 gtggatcgct aagcaccgca taggcctggg cgatcagggc ggtgcgatca gtggcctcaa
      901 tggcgtcggc atttcggtcc ggatggtggc gctgcatcag gcttttgtag gcggcccgga
      961 taacctcggc actggcccgg gggctcacct ccagaatgtc gtaatggctc aaacctggca
     1021 tggctggtca ctgcccccgc atcaatcaag gcgcctgaac acggtgatct gcccgtcttc
     1081 gcgcagccgg aactcgcggg cgttgaccga ttcaacctga ttcacctcgc ggccgatctg
     1141 accggcactt ttgaaatgcc agatgatgcg cgaatcctgg tacgcccata gcccatactg
     1201 caacacgcct ccgttgtcat tcttgatctc ccattctccg ttgtcataca gatgcagcgg
     1261 tgttgtgagt ttggaagatt gccacttgcc gacaaaagac gcagcgtcgg gctgcacctg
     1321 acccttgcag gccccaagca acaggccgac cagcaccgcc agcacgctga attttccaaa
     1381 ccggccaggt gaacgcctgc gcattatgaa aaccttgcca gatcaattgc tgctgggcgt
     1441 tcgcagcacg ttttgccatt tggcgcgctc ggcctcaatg cgcctgcgct cggcttcggc
     1501 actgagacgg gcttcctctt cctttagccg cgcctcttgc aacagggcct gctgggcttt
     1561 tttctcagcg tgctgcagtt caattgatac ctgctcgcca cgtttgcggg cctcggcttc
     1621 aaagttgcgc tcggcctgtt cagctttctt cttctcttct tcggccagcc tttgggccgt
     1681 cttggcatcg tcgtttttgc gccgctcagc atccatcaac gccgcttcag cccgactggc
     1741 cgggcgcacg ccccaggtct ggtaatactc ctgcagaaac accttgtcct cggtctggct
     1801 gcgcgcgccg actgaatcat cagatgggcg cggcgacagg accagcatga aaaccagctt
     1861 gaaaaccatg cccagcgccg ccagggtgcc cagcaccagc agcgcccttt tcatggtctt
     1921 gaaccagcgt gatgccgccg ggccagtggc tgcaccacca gggccgtcaa gctgtccggt
     1981 cagcaggtcg gccttgagcc cgagcgcatc ggcgcgcagc gtcatggcct cggcccgcat
     2041 catcaaggca tcagcccgaa tggccgccgt attgctgctg gcagccggtg tcacctgcaa
     2101 ggctgaaccg gccctggccg attcggcgcg cgagtgcagg ccagcatcgt aagcatcgcg
     2161 agaactgggg gcagaaagcg cgctgaacgc caccttgagc aggcgcaact gcgtggcgta
     2221 gtcttcgcgg ctcaacgtcg actgctgact ttccagcaag cgctgcagcc gctcataggc
     2281 ggcccggatg tccgcataat ctgcgtcctg ctgcacttcc aacaattcgt atagcgattt
     2341 cttttgagtc atccgaagta ccccgcgcaa tagcttgacc ttggcttgtg atggtatccg
     2401 ctaagctttt accggacaat gacgaccagg caaatcgtca accaactcac cgtgatagaa
     2461 ccggcaacat ggcaaaaacc gcaacaatca ggccaagtca cctgatgctg cc
//
LOCUS       DAOSLL010000658         2507 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_36057_length_2507_cov_5.620718, whole genome shotgun sequence.
ACCESSION   DAOSLL010000658 DAOSLL010000000
VERSION     DAOSLL010000658.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36057_length_2507_cov_5.620718"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PLB25_20475"
     CDS             complement(<1..370)
                     /locus_tag="PLB25_20475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE43981.1"
                     /translation="MTTAAEILHLQEHGLYSSDAEHDACGVGFVAHIKGVKSHDIVKN
                     ALKILENLDHRGAVGADKLMGDGAGILIQLPDALYREEMAALGVTLPPPGEYGVGMVF
                     LPKEHASRQACEQEVERAIKV"
     gene            complement(550..1248)
                     /locus_tag="PLB25_20480"
     CDS             complement(550..1248)
                     /locus_tag="PLB25_20480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007835236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HOE43982.1"
                     /translation="MARLPRLTLPDYPHHVIQRGNNHQLIVNTPADYRRLLEVLEEAA
                     KRCQVAIHAYVLMSNHFHLLATPQTVQGLPQMMQSLGRSYVRYFNTTQQRSGTLWEGR
                     YKSTLIQGERYLLACMAYIDLNPVRAGMVARPQDYPWSSHGHYIGMRSDRLITPHALF
                     WELGNTPFAREAAYAELVQAGINSVQQAALTDATLSGWALGEADFVANLQKRTKRRVS
                     KTSAGRPFSVNKSS"
     gene            complement(1292..2170)
                     /locus_tag="PLB25_20485"
     CDS             complement(1292..2170)
                     /locus_tag="PLB25_20485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010462121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HOE43983.1"
                     /translation="MRQPKPAQPRVSHSPTHPLADEIRPGQSLELLKELHILTREGRL
                     NQDSRRKLKQVYHLFQFIEKLLLELADNGTAGITLADHGAGKSYLGFILYDLFFKQRP
                     GGHIYGIETRPELVQKSNELATRLGFERMSFLNLTVAESALSERLPGRIDMVTALHAC
                     DTATDDAIAFGLQKQARFMVLVPCCQAEVARVLSQDKALSLSRTPLAELWRHPLHTRE
                     MGSQITNVLRCLYLEACGYQVTVTELVGWEHSLKNELIMARHTGQPKHAAAQRLLALL
                     TELGLTSLLKSRFPVK"
     gene            complement(2255..>2507)
                     /locus_tag="PLB25_20490"
     CDS             complement(2255..>2507)
                     /locus_tag="PLB25_20490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005792770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1415 family protein"
                     /protein_id="HOE43984.1"
                     /translation="GLDGVLQIASLHPQYQFADTDADDITNFTNRSPYPTLHLLREDS
                     IDRAVAAFPNPEAIYETNMQTLQALGQSGWDALKVGSSP"
BASE COUNT          504 a          707 c          751 g          545 t
ORIGIN      
        1 cgaccttgat ggcacgctct acctcttgct cgcaagcctg gcgcgatgca tgctctttgg
       61 gcaaaaacac catgccaaca ccatactcgc ccggtggcgg caaggtcacc cccaacgccg
      121 ccatctcttc gcggtacaac gcatcgggca actgaatcag gatgccggca ccgtcgccca
      181 tcagcttgtc cgcccccacc gcaccccggt ggtccagatt ctcaagaatc ttcaaggcgt
      241 ttttgacaat gtcgtgcgac ttgaccccct tgatgtgcgc cacaaaacca acaccgcaag
      301 catcgtgctc ggcatcagat gaatacaaac catgttcctg gagatgcaag atctcggcag
      361 ccgtcgtcat tggcgcgctc ctgtaaatgt caaaaaagaa gtaactctac cctattgcaa
      421 caagagtgcc aaagaaatta attggggtca gagtcgaatt aattgatcaa tttgcgattc
      481 tgagttttat tggggacata tcaattttgt agctgtcagc gcttacggtg tatgggctag
      541 agttgatttt tagctggatt tattgaccga gaatgggcgt ccagcgcttg tcttgctgac
      601 gcgacgcttt gttctttttt gcaaattcgc cacaaaatct gcctcgccca gcgcccagcc
      661 gctcagggtg gcatcggtca aagcggcctg ctgcaccgag ttgatgcctg cttgcaccaa
      721 ttcggcatat gccgcctcac gcgcaaaggg tgtgttgcca agctcccaaa acagtgcatg
      781 gggcgtgatc agccgatcag aacgcatgcc gatgtagtga ccatggctgg accatggata
      841 gtcttgtggt cgcgccacca tgccggcacg caccgggttg aggtcgatgt aggccatgca
      901 ggccagcaaa tagcgctcgc cctggatcag ggtggacttg tagcgcccct cccacagcgt
      961 gccactgcgc tgctgggtcg tgttgaagta acgcacatag ctgcgcccca gcgactgcat
     1021 catctgtggc aagccctgca ccgtttgcgg tgttgccagc aagtggaagt ggttgctcat
     1081 cagcacatag gcgtggatgg cgacctgaca ccgtttggcg gcttcttcca gcacctccag
     1141 caggcggcgg tagtcggccg gggtgttgac aatcaattga tggttgttac cgcgctggat
     1201 cacatggtgc ggatagtctg gcagtgtcag gcgcggcaat ctggccatgt tgaagtcctt
     1261 tgctgactga ataattcgac tctgacccca attatttcac tggaaagcgc gatttcagca
     1321 ggctggtcag gccgagttcg gtcagcaggg ccagcagccg ctgcgcggcg gcatgcttgg
     1381 gctgaccggt atggcgggcc atgatcagct catttttcag gctgtgttcc cagcccacca
     1441 gctcggtgac cgtgacctgg tagccgcagg cctctaggta caggcagcgc agcacgttgg
     1501 tgatctggct gcccatttcg cgggtgtgca gcggatggcg ccagagttcg gccaatggcg
     1561 tgcgcgacag cgacaaggct ttatcctggc tcagcacccg cgccacctca gcttggcaac
     1621 aaggcaccag caccatgaag cgcgcctgct tttgcaagcc gaaggcgatg gcatcgtcag
     1681 tggcggtgtc acaagcatgc agggcagtga ccatgtcgat gcgcccgggc agccgttctg
     1741 acagggcgga ctcggccacc gtcaggttca aaaatgacat ccgctcaaaa cccaggcgcg
     1801 tcgccagctc attcgacttc tgcaccaatt cagggcgggt ttcgatgccg tagatgtgac
     1861 cgccgggccg ttgtttgaaa aacaggtcgt acaaaatgaa accaagatag gacttgccgg
     1921 caccatggtc ggccaatgtg atacctgcag tgccgttgtc ggcgagttcg agcagcagct
     1981 tttcgatgaa ttgaaacagg tgataaacct gcttgagctt gcgccgggaa tcctggttaa
     2041 gtcggccttc gcgggtcagg atgtgaagtt ccttgagcaa ctccagcgat tgcccagggc
     2101 ggatctcgtc tgccagcggg tgggttgggg aatggctgac acggggttgg gcgggtttgg
     2161 gttggcgcat ggggcgatga tgccatgccc ggcgtttggg gcttgctggc tctgaaacgg
     2221 ctgtcggctt gacccccctg gcgacctcgc tgcgctatgg cgaggatccc accttcagcg
     2281 cgtcccagcc cgattggccg agcgcctgca gcgtttgcat gttggtctca taaatcgcct
     2341 cagggtttgg aaaagccgcc accgcccggt cgatgctgtc ttcgcggagc aaatgcaagg
     2401 tcgggtacgg cgagcggttg gtgaaattgg tgatgtcatc ggcatcggta tcggcaaact
     2461 ggtactgcgg gtgcagcgag gcaatctgca gcacgccgtc aaggccg
//
LOCUS       DAOSLL010000659         2499 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_36230_length_2499_cov_3.851882, whole genome shotgun sequence.
ACCESSION   DAOSLL010000659 DAOSLL010000000
VERSION     DAOSLL010000659.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36230_length_2499_cov_3.851882"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="PLB25_20495"
     CDS             complement(<1..340)
                     /locus_tag="PLB25_20495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HOE43985.1"
                     /translation="MGVVGVIGLGYVGLPLVVEFGKHFRTIGFDISVPKVESCQRGVD
                     PSRELADEDLALASQAVYTADPAMLSEADIIIIAVPTPVDNAHIPDFKPLIGSSTSAG
                     RHMKKGAIVVY"
     gene            436..1446
                     /locus_tag="PLB25_20500"
     CDS             436..1446
                     /locus_tag="PLB25_20500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HOE43986.1"
                     /translation="MKILLTGAAGFIGMTASLKLLARGDEVVGIDNLNNYYDVTLKES
                     RLNRLLTHSNFRFVRLDVADQAGMTALFAAEKFDRVIHLAAQAGVRYSLQNPHAYIES
                     NIVGFTNILEGCRHSKVQHLVYASSSSVYGGNTKMPFSEHDSVDHPVSLYAASKKANE
                     LMAHTYSHLFGLPTTGLRFFTVYGPWGRPDMALFLFTKAILDNQPIDVFNHGNMMRDF
                     TFVDDIVEGVVRVLDRIPQPNLAYDAVAADPATSSAPYRVFNIGNNNPVRLLDFIACI
                     EDALGKKAEKRLLPLQSGDVPATYANTDALNDWVGFVPGTSVQDGIGRFVAWYRDYYK
                     VS"
     gene            complement(1556..>2499)
                     /locus_tag="PLB25_20505"
     CDS             complement(1556..>2499)
                     /locus_tag="PLB25_20505"
                     /inference="COORDINATES: protein motif:HMM:NF023410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EpsI family protein"
                     /protein_id="HOE43987.1"
                     /translation="FIVVSILVPVIANWARAYMIVMLGHLSSNKIATGVDHLIYGWLF
                     FGIVILLLFMIGAHWAEPEPSADASRDLVAEKAVQPISGHTPAPSYWRAHRSYVLALV
                     LLVASPHLARWAIEASDRAAAPQLPAAPVLASAWSLAPSPVAVFKPAFQNPSATINNT
                     YQSHSKQVGLYMGYYRQQDYSRKLVSSDNVLVKSKDSQWAQISVGIRNMQIDGQTRSF
                     RTAELRGSPVPGLLSDNYLLVWQIYWVNGTWTISDMMAKGYGALYRLLGRGDDSAVII
                     LYTIKGEAGEGQAALEAFVQANSTEISTLLQQTRARR"
BASE COUNT          578 a          652 c          700 g          569 t
ORIGIN      
        1 cgtacacgac gatggcacct tttttcatgt ggcgaccggc gctggtgctg gagccaatca
       61 agggtttgaa gtctggaatg tgggcgttgt ccaccggtgt cggcacagca atgataatga
      121 tgtcggcctc tgacagcatg gcagggtctg cggtatagac ggcttggctg gccaaagcga
      181 ggtcttcatc ggccagctcc cgagagggat ccacgccgcg ctggcaagac tcaactttgg
      241 gcactgaaat atcaaaaccg atagttctga agtgtttgcc aaattcgacc accagaggca
      301 aaccgacata acccagacca ataacgccta cgacacccat agctactcct ttgatgctcg
      361 gtttgctgaa cggcaaacta tacctgcaca atggcggcta agatgatgcg ttgaactatt
      421 aaaggcatat ttcaaatgaa aattctattg accggtgccg ctggctttat tggcatgacc
      481 gcgtcgctga aattgcttgc gcgaggcgat gaagtggtgg gaattgacaa tctgaacaac
      541 tattacgacg tgacactgaa ggaaagtcgt ctgaatcgcc tgttgacgca ttctaatttc
      601 cggtttgtca ggctcgatgt ggccgatcag gctggcatga ccgcactgtt tgctgcagag
      661 aagtttgacc gcgtcatcca tctggccgct caggctggcg tgcgctattc gctgcaaaat
      721 ccccatgcct atattgagag caacattgtt gggttcacca acattctgga aggctgccgc
      781 cacagcaagg tgcagcacct ggtgtacgct tcaagtagca gcgtgtatgg cggcaatacc
      841 aaaatgccgt tctcagagca tgacagtgtg gaccacccgg taagcttgta tgcggccagc
      901 aaaaaagcca atgagctgat ggcgcatacc tacagccatt tgttcggcct acccaccaca
      961 ggcttgcgct tcttcacggt gtatggtccc tggggcaggc ccgatatggc gttgtttttg
     1021 tttaccaaag cgattttgga caatcaaccg attgacgtgt ttaaccacgg caacatgatg
     1081 cgcgacttca cctttgtcga tgacattgtg gagggcgtgg tgcgggtgct ggaccgcata
     1141 cctcaaccca acctggcata cgacgctgtg gcggctgacc cggctaccag cagtgcacct
     1201 taccgagtgt tcaatatcgg caataacaac ccggtgcgtt tgcttgactt tattgcctgc
     1261 atagaagatg cgctgggcaa aaaggccgag aagcgcttgc tgccgctgca aagtggtgat
     1321 gtacccgcca cttacgccaa caccgatgca ctcaatgatt gggtgggttt tgtgcccggc
     1381 accagtgtcc aggatggcat tgggcgcttt gtggcgtggt accgggatta ctacaaggtg
     1441 tcatagtctg caagccccac accagccttt cgggtgaaag atcgcacttg ctgcggggtg
     1501 tcaaaaggtc cgcgaaagct acacaaggct ttggtttcgt ccagtttgaa ggactttagc
     1561 gacgagcccg ggtttgctgc agcaatgtgc tgatctcagt ggagttggct tgcacaaacg
     1621 cctcaagcgc ggcttgtcct tcgcccgcct cgcccttgat cgtgtagaga atgatgactg
     1681 cagaatcatc gcccctgcca agcaatcggt acagcgcacc gtaacccttg gccatcatgt
     1741 cactgatggt ccaggtgcca ttaacccagt agatttgcca caccagcagg tagttgtctg
     1801 acaaaagccc gggtacgggc gagccacgca gttcggcagt gcgaaacgat ctggtttgtc
     1861 cgtcgatttg catgttgcgg atgccaactg atatctgagc ccattgggaa tcttttgact
     1921 tgaccagcac attgtcggaa ctgaccagct tgcggctgta gtcttgctgg cggtaatagc
     1981 ccatataaag accaacctgc ttggaatggc tttggtaggt gttgttgatg gtcgccgagg
     2041 ggttttggaa ggcaggttta aagacagcca ctggactagg tgccagagac caagccgatg
     2101 ccagaaccgg agctgctggc aattgaggtg cagcagcccg gtcggacgct tcaatggccc
     2161 agcgcgccaa atgtggtgat gcgacaagta acaccagtgc aagtacataa ctgcggtggg
     2221 ctcgccagta gctaggcgct ggcgtgtgac ctgatattgg ctgcacagcc ttttctgcca
     2281 ccaggtcacg gctggcatct gcacttggct caggttcagc ccagtgcgct ccaatcatga
     2341 acagcagcag aatgacgatg ccaaagaaca gccagccata aatcagatgg tcaacgccgg
     2401 tggcgatttt gttgcttgac agatgcccta gcatgacgat catgtaggcg cgcgcccagt
     2461 tggcgatcac cggaaccaga atcgagacca caatgaaca
//
LOCUS       DAOSLL010000660         2494 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_36323_length_2494_cov_5.242722, whole genome shotgun sequence.
ACCESSION   DAOSLL010000660 DAOSLL010000000
VERSION     DAOSLL010000660.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36323_length_2494_cov_5.242722"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1624
                     /locus_tag="PLB25_20510"
     CDS             <1..1624
                     /locus_tag="PLB25_20510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010466154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HOE43988.1"
                     /translation="GDARLEDVLVHSLAATQGATATPAANAPASAAPTQSAAPTEELL
                     RWKSLNLNGLTVALKPGSAPRVEVSSTVLSDFYARLILSETGRLSLQDVMKPAAATNA
                     AGVPPASAAASSQFAIQSGASSATPASAAGQNDVQTAMAPQMRFGPISLVAGQVHFTD
                     RFIKPNYSANLTELTGRLGAFSSQATDGQVQLADLDLRGRVEGSATLEVQGKVNPLAK
                     PLALDIKGRVRDLELAPLSPYAVRYAGYGIERGKLSVDIAYLVQPDGMLTASNNIVLN
                     QLQFGDKVPDSAASLPVKLAVALLADRNGVIDLNLPVSGSLNDPQFRLLPIVFKIIGN
                     LIAKAITAPFSLLAGALGGAGDEASAVNFAAGSSTLTAQARAGLDKIAKALADRPALK
                     ITVVGSASLEQEREAFKRAQLQNLLLAEKRRSDKGAPAEPTASDPVVPQQEYPALLKA
                     VYKRADFAKPRNLVGLAKDLAAAEMEALLLANLTATEADMAALALKRAVVVRDYLASQ
                     KLPADRLSLGAAKAVTPDAKWQPRADLNLTLE"
     gene            1743..>2494
                     /locus_tag="PLB25_20515"
     CDS             1743..>2494
                     /locus_tag="PLB25_20515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HOE43989.1"
                     /translation="MFSFSTHNKADPTSDAPAAPAKTPEAHPLDALTRGAFSAQTSGE
                     RMACIRDWLTSSPTAEQMQQVFKELSGKDKGAAKLLREKLDDIKRSKAQAGMALEWAD
                     KGQALLALAKLNLADALAWQRDAAKAGAPLSKEPLAAIKVQLVERVRAIEDLQHRVQV
                     QREAAVLVAQRIEVLSTKPWRDALAVQEALSQDVANWQTEASALTGDANWPSVDTRFP
                     PQLQASQAQLGLVWEAFQEALTQARLAAEDAGA"
BASE COUNT          499 a          779 c          781 g          435 t
ORIGIN      
        1 tggcgatgcc cgcctggaag acgtgctggt gcacagcctt gccgccaccc agggcgcaac
       61 ggcaaccccg gcagccaatg cgccagcgag cgccgcgcca acgcagtcgg cggcaccgac
      121 agaagaattg ttgcgctgga aaagcctcaa cctgaacggc ctgacggtgg ccttgaagcc
      181 gggctcggca cccagggtcg aggtgtcatc gaccgtcttg agcgactttt atgcacggct
      241 gatcctgagc gaaaccgggc ggctcagcct gcaggatgtg atgaaacccg cagccgccac
      301 aaatgccgct ggcgtgccac ctgccagcgc tgccgccagt tctcaatttg ctattcaatc
      361 aggagcttcc agcgcaacac cagcaagcgc tgcaggccaa aatgatgttc aaactgcaat
      421 ggcaccgcaa atgcgttttg ggccgatcag tctggtcgct ggccaggtgc atttcaccga
      481 ccgcttcatc aagcccaact actcggccaa cctgaccgaa ctcaccggtc gcctgggtgc
      541 cttctcaagc caggccaccg acgggcaggt gcagttggca gaccttgatt tgcgtggccg
      601 ggtcgaaggc agcgccacac tggaggtgca aggcaaggtc aacccgctgg ccaagccgtt
      661 ggcgctcgac atcaagggcc gggtgcgcga cctggaactg gcaccgctgt cgccctatgc
      721 ggtgcgctac gccggctatg gcatcgagcg cggcaagctc agtgtcgata tcgcctacct
      781 ggtgcagccc gatggcatgc tcacggccag caacaacatt gtgctcaatc agcttcaatt
      841 tggtgacaag gtgccggatt cggccgccag cctgccggtc aagctggccg ttgcgctgct
      901 ggctgatcgc aacggggtga ttgacctcaa cctgccggtc agtggttcgc tcaacgaccc
      961 gcagttccgg ctgctgccga ttgtgttcaa gatcattggc aacctgattg ccaaggcgat
     1021 caccgcgccg ttcagcctgt tggcgggcgc tctgggtggt gccggtgacg aggcaagcgc
     1081 ggtgaatttt gcagccggtt caagcaccct gacagcacag gcacgcgccg ggcttgacaa
     1141 gattgccaaa gccctggctg atcgcccggc cctgaaaatc acggtggttg gcagtgccag
     1201 cctggagcaa gagcgcgagg ccttcaagcg ggcccagttg caaaatctgc tgctggcaga
     1261 aaaacgacgt tccgacaagg gcgcaccggc cgagccgact gcatcggacc cggttgtgcc
     1321 gcagcaggag tacccggcgc tgctcaaggc ggtttacaag cgcgctgatt ttgccaagcc
     1381 gcgcaacctg gtcggactgg ccaaggacct tgcggcggct gaaatggaag ccttgttgct
     1441 ggccaatctg actgccactg aagccgacat ggccgcactc gcgctcaagc gcgctgtggt
     1501 ggtgcgtgac tacctggcca gccaaaaatt gccagcggac cggctgtccc tgggtgccgc
     1561 caaggcggtt acgccagatg ccaaatggca gccgcgcgcc gacctgaatc tgacccttga
     1621 atagccctgg cattggtgtc ccaaatgttt tttgaaagca tcgcgaaaac acggcgaaaa
     1681 tagccgattg ttcccacggc tgtgctgtgg gcattttttt attgtgtgtg tgtgccatgt
     1741 cgatgttttc cttctctacc cataacaaag cagacccaac ttctgacgct cctgctgcgc
     1801 cagccaaaac ccctgaagcc cacccgcttg atgcactgac ccgaggtgcg ttttcggccc
     1861 agacctcggg cgagcggatg gcctgcatcc gcgactggct gaccagcagc ccgacggccg
     1921 aacagatgca gcaggtgttc aaggaactca gtggcaagga caagggcgcc gcaaagctgt
     1981 tgcgtgaaaa gctcgacgac atcaagcgca gcaaggcaca agctggcatg gcactggaat
     2041 gggccgacaa ggggcaggca ctgctggcgc tggccaagct gaacctggcc gatgcattgg
     2101 cctggcaacg cgacgctgcc aaagccggtg cgcccctgag caaggagccg ctggcagcca
     2161 tcaaggtgca actggttgag cgggtgcgtg ccatcgaaga cctgcagcac cgggtgcagg
     2221 tgcagcgcga agccgccgtg ctggtggcgc aacgcatcga agtcctgtcc accaagccat
     2281 ggcgcgacgc gctggcggtg caagaggcgc tcagccagga tgtggccaac tggcaaaccg
     2341 aggccagcgc gctcaccggt gatgccaact ggcccagtgt cgatacccgc tttccacccc
     2401 agttgcaggc ctcgcaggcg cagcttggtc tggtctggga ggcttttcag gaggccctga
     2461 cgcaggctcg gctggcggca gaagatgctg gcgc
//
LOCUS       DAOSLL010000661         2488 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_36422_length_2488_cov_5.247842, whole genome shotgun sequence.
ACCESSION   DAOSLL010000661 DAOSLL010000000
VERSION     DAOSLL010000661.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36422_length_2488_cov_5.247842"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="PLB25_20520"
     CDS             <1..493
                     /locus_tag="PLB25_20520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011794319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HOE43990.1"
                     /translation="TGKTYLALVRGQWPGKLKVLDKPLHKYLQDSGSAAAGERRVRVV
                     SRDDPNGLPSVTLVRMRVSSHDFSLLEVTIKTGRTHQIRVHLASEGYPIVGDDKYGDF
                     DFNKSLARSASEGGLKRMFLHAWRLQLNHPATGVRLELQADLPPELTTFLAAALPQSL
                     NSP"
     gene            503..1162
                     /locus_tag="PLB25_20525"
     CDS             503..1162
                     /locus_tag="PLB25_20525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HOE43991.1"
                     /translation="MTDRRFDLIAFDWDGTLFDSTAIITRCIQAAVRDVGGTVPSDSQ
                     ASFVIGMGLMQALAHAAPDVPREAYPLLGDRYRHHYFAIAHEICLFDGILPMLAELKA
                     RGFLLTVATGKSRRGLDESLSSVALAGMFDASRTADQTAGKPNPLMLRELMAEFDVSA
                     RRTLMIGDTTHDLQMARNAGCSSVGVTYGAHEPEAFQTLGPLFVAHNVAQLHDWLLAQ
                     A"
     gene            1203..1571
                     /locus_tag="PLB25_20530"
     CDS             1203..1571
                     /locus_tag="PLB25_20530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HOE43992.1"
                     /translation="MTAKVFLCQSSDLEDSGSAVPFEVRHFSQSCAAFAVRYEGRVYA
                     YLNRCSHVPMEMDYQPNRFFDASGHWLMCATHGAMYAPQTGQCLMGPCRGGLVRIETS
                     ETEGVVHWHTSDKFQPDSNP"
     gene            1568..>2488
                     /locus_tag="PLB25_20535"
     CDS             1568..>2488
                     /locus_tag="PLB25_20535"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HOE43993.1"
                     /translation="MTEPSDANTSGAQPSLDDPSNQAQTPVDQGAKATKNVADQVSET
                     VSWERSTLEKLAFAALHEQKLARRWRTFVRLAWLVFMIGLVWIGVNQGGSSQNVSTPH
                     TAVVEIKGEVAAGGQASAEFVVAAMRTAFEDAGAQAVVLLINSPGGSPVQAGIINDEV
                     RRLKALHKKPVFAVVEESCASAAYYIAVSADQIFVDKASIVGSIGVLMDGFGFTGLMD
                     KVGIERRLMTAGENKGFLDPFSPQTAKQREYAQAMLNQIHQQFIDAVKAGRGEHLKET
                     PETFSGLFWSGQQAIELGLADQLGSLDFVAR"
BASE COUNT          508 a          688 c          779 g          513 t
ORIGIN      
        1 taccggcaag acctatctgg ccctggtgcg tggccagtgg cctggcaaat tgaaggtgct
       61 ggacaaaccc ttgcacaaat acctacagga cagtggcagt gcagcggcag gtgagcggcg
      121 ggtgcgggtg gtgtcgcgcg acgacccgaa tggcctgccc tcggtcacgc tggtcaggat
      181 gcgcgtttcg tcccatgact tttcgctgct ggaggtcacc atcaaaaccg gacgtaccca
      241 tcaaatccgg gtgcacctgg ccagcgaggg gtatccgatt gtcggcgacg acaagtatgg
      301 cgacttcgat ttcaacaaat cgctggcgcg cagcgcaagc gagggtgggc ttaaacgcat
      361 gtttttgcac gcctggcgct tgcaattgaa tcaccctgcc accggtgtgc ggcttgagtt
      421 gcaggcagac ttgccccccg agttgacaac ctttctggcg gctgctttgc cgcaaagcct
      481 gaattcacca tgagccgaca agatgacaga ccgacgcttt gacctgattg cctttgactg
      541 ggatggcacc ctgtttgact ccaccgccat catcacccgc tgcatccagg cggcggtgcg
      601 tgatgtcggc ggcacggtgc ccagcgacag ccaggcatcg tttgtgattg gcatgggttt
      661 gatgcaggcg ctggcacatg ctgctcccga tgtgccgcgc gaggcatacc cgttgctcgg
      721 tgatcggtat cgtcaccatt actttgccat tgcgcatgaa atctgcctgt ttgacggcat
      781 cttgccgatg ttggcagaac tgaaggcgcg cggcttcttg ctgacggtgg caaccggcaa
      841 aagccgccgt ggacttgatg agtccttgag cagcgtggca ttggcaggca tgtttgatgc
      901 ctcgcggacc gctgaccaga ccgcgggcaa gcccaatccg ctgatgctgc gtgaactgat
      961 ggctgaattt gatgtcagcg caaggcgcac cctgatgatt ggcgacacca cgcacgacct
     1021 gcaaatggcg cgcaatgccg ggtgcagcag tgtcggcgtg acttatggcg cgcatgagcc
     1081 ggaagctttt cagacgctgg ggccgttgtt tgtagcgcac aacgtcgcgc agttgcatga
     1141 ctggctgctg gcgcaggcct gagcaagacg cgtcaattca ccatcagccc tgaggaatcg
     1201 tcgtgacagc aaaagtgttt ctgtgccaat cctctgatct agaggacagc ggtagcgccg
     1261 tgccgttcga ggtgcgtcac ttttcgcaaa gctgcgcggc ctttgccgtg cgctatgagg
     1321 gccgggtcta tgcctacttg aaccgctgct cgcatgtacc gatggaaatg gactaccagc
     1381 ccaaccggtt ttttgatgcc agcggtcact ggttgatgtg cgccacccat ggtgccatgt
     1441 acgcaccgca aaccggtcag tgcctgatgg ggccatgccg gggtggcctg gtcaggattg
     1501 agaccagcga gactgaaggg gtcgtgcact ggcatacttc cgataaattt caacctgaca
     1561 gcaacccatg acagaacctt cagatgccaa caccagcggc gcacagcctt cgctggatga
     1621 tccgtcaaat caagcgcaaa cccctgtgga tcaaggggca aaagctacaa agaacgtagc
     1681 tgatcaggta tctgaaacgg tcagttggga acgcagcacg ttggaaaaac tggcctttgc
     1741 ggccctgcat gagcagaaac tggcgcgccg ctggcgcact ttcgtgcgcc tggcctggct
     1801 ggttttcatg attgggctgg tctggatcgg tgtcaaccag ggcggcagct cgcaaaatgt
     1861 cagtacgccc cacactgccg tggtcgaaat caagggtgaa gtcgccgccg gtggccaggc
     1921 cagcgcggaa tttgtggtgg ctgcgatgcg caccgccttt gaagatgctg gagcccaggc
     1981 agtggtgctg ttgatcaatt cgccaggggg cagcccggtg caggcgggca tcatcaacga
     2041 cgaagtcagg cgactcaagg cgctgcacaa gaaaccggtt tttgcggtgg tggaggagtc
     2101 ttgtgcttcg gctgcctatt acatcgcggt gtcggcagac cagatatttg tcgacaaagc
     2161 cagcattgtg ggcagcatcg gcgtgctgat ggacggcttt ggctttaccg gcctgatgga
     2221 caaggtgggc attgagcggc gcctgatgac ggcaggcgaa aacaaggggt ttctggaccc
     2281 gttcagcccg caaactgcaa aacagcggga gtatgcccag gccatgctga accagattca
     2341 ccagcagttc attgatgcag tgaaagccgg tcgcggtgag catctgaaag aaacgccaga
     2401 aaccttcagc gggttgttct ggagcggcca gcaagccatt gaactcggcc tggccgatca
     2461 gttgggcagc ctcgattttg ttgcacgg
//
LOCUS       DAOSLL010000662         2466 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_36846_length_2466_cov_3.917462, whole genome shotgun sequence.
ACCESSION   DAOSLL010000662 DAOSLL010000000
VERSION     DAOSLL010000662.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36846_length_2466_cov_3.917462"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="PLB25_20540"
     CDS             <1..307
                     /locus_tag="PLB25_20540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013543174.1"
                     /note="with UgpACE is involved in the uptake of
                     glycerol-3-phosphate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HOE43994.1"
                     /translation="QSASHKRTGYLPITTAAFNLTEKSGFYKENPGTDVAPTQMIRKV
                     TDKSRGIRLGNYVQIRAIEDEELEQVWGGKKSAKEALESIVKRGNELLERFEKANKK"
     gene            462..1343
                     /gene="ugpA"
                     /locus_tag="PLB25_20545"
     CDS             462..1343
                     /gene="ugpA"
                     /locus_tag="PLB25_20545"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015866909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HOE43995.1"
                     /translation="MEKRVFFRSNWLPWLLIAPQVVVIVLFFFWPAAQALLQSVQQSD
                     AFGTSVEFVGLENFRNLWHDETYLASFYTTAVFSTLVAVVGITVSLLLAVFADRIVKG
                     ALAYKTLLIWPYAVAPAVAGVLWLFMFAPSVGVVSYWLRAIGVDWNHLLNGTHAMTLV
                     VMAAVWKQISYNFLFFLAGLQSIPKSLIEAAAIDGARPWRRFWTIVFPLLSPTTFFLL
                     VINIVYAFFDTFAIIDAATQGGPGKETAILVYKVYFDGFKAMDLGGSAAQSVVLMVIV
                     VALTVVQFRFVEKKVNY"
     gene            1343..2194
                     /gene="ugpE"
                     /locus_tag="PLB25_20550"
     CDS             1343..2194
                     /gene="ugpE"
                     /locus_tag="PLB25_20550"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006394817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HOE43996.1"
                     /translation="MVERRPFLTFLSHAVLILGVLVVAFPLYLTFVASTQTSQAIVQT
                     PMPLLPGGYLIENYTAAWNGTGGGSGSKAPVGQMMLVSLVSALVISLGKIAISLLSAF
                     AIVYFRFPFRMLVFWAIFITLMLPVEVRIGPTYKVVSDLGMLNSYAGLTIPLIASATA
                     TFLFRQFFLTVPEELVEAARIDGAGPMRFFKDILVPLSKTSIAALFVIQFIYGWNQYL
                     WPLLVTTSEDMYPVVIGIKRMISGGDSEMQWNIVMATAVLAMLPPALVVMLMQKWFVK
                     GLVDTEK"
BASE COUNT          455 a          660 c          715 g          636 t
ORIGIN      
        1 gcagtcagcc agccacaaac gcaccggtta cctgcccatc accaccgccg cgttcaacct
       61 gactgaaaag tccgggtttt acaaggaaaa tcccggcacc gatgtggcac ccacccagat
      121 gatccgcaag gtgaccgaca aatctcgcgg catccgcctt ggcaattatg tgcagatccg
      181 tgccatcgag gacgaggaac tggagcaagt ttggggtggc aagaagagcg ccaaggaagc
      241 gcttgagtcg atcgtcaaac gtggcaacga gttgctggag cggtttgaaa aggccaacaa
      301 gaagtgatgg ccttttttga gcttgcggtg ctgccaagcg gtccttgtga atcctcaggg
      361 ccgcgcatgc gccaacccat gcaacgcggt atctgccctt gcccggcaac gaccgctgcc
      421 gaactttggc aggccggttt tttcgaatag cgcagtaact cgtggaaaaa cgcgtctttt
      481 tccgttccaa ctggttgccc tggttgctga tcgcacccca ggtcgtggtg atcgtgctgt
      541 ttttcttttg gccggcggcc caggcgctgc tgcagtcagt gcagcaatcc gatgcgtttg
      601 gcacatcggt ggagtttgtc gggctggaga actttcgcaa cctctggcac gatgaaacct
      661 acctggcatc gttttacacc acggcggttt tttcaacgct ggtggccgtt gtcggtatca
      721 ccgtgtcatt gctgttggcg gtgtttgccg accgcattgt caagggtgcg ttggcctaca
      781 aaaccctgct gatctggccc tatgccgtcg ccccggcggt ggccggcgtg ctttggctgt
      841 tcatgtttgc gcccagtgtg ggtgtggtgt cgtactggtt gcgcgccatc ggggtcgact
      901 ggaatcacct gctcaacggt actcacgcca tgacgctggt tgtgatggct gccgtgtgga
      961 agcagatttc ctacaacttt ttgttctttc tggcagggct gcagagcatc cccaaatcct
     1021 tgattgaagc agccgccatt gacggtgcca ggccctggcg gcgcttttgg accattgtgt
     1081 ttccgctgct gtcgcccacc acctttttct tgctggtcat caatatcgtt tacgcctttt
     1141 ttgacacctt tgccatcatc gatgctgcca cccagggtgg tccgggcaaa gaaaccgcca
     1201 ttctggtcta caaggtgtac tttgacggct tcaaggcgat ggacttgggt ggctcggccg
     1261 cgcagtcggt ggtgttgatg gtgattgtgg tggcgcttac cgtggtgcag ttccggtttg
     1321 tcgagaagaa agtgaattac tgatggttga gcgccgcccg tttctgacct ttctgtcaca
     1381 cgccgtgttg attctgggtg tactggtggt cgcgtttccg ctgtatttga cttttgtggc
     1441 ttctacccag acctcgcagg ccattgtgca gacacccatg ccgttgctgc cgggcgggta
     1501 tctgattgaa aactacaccg cagcctggaa cggcactggc ggtggctctg gctccaaggc
     1561 accggtcggg caaatgatgc tggtcagtct ggtttctgcg ctggtcatct ctttgggcaa
     1621 aattgcaatt tctttgttgt cggccttcgc cattgtctat ttccgttttc cgtttcgaat
     1681 gttggtcttc tgggcgatct ttatcaccct gatgctgccg gtggaggtgc gaattggccc
     1741 cacctacaag gtggtatccg atctgggcat gctcaacagt tatgcggggc tgacgattcc
     1801 gctgattgcc tcggccacgg ccacgttttt gttccggcag ttttttttga cggtgcccga
     1861 agaactggtg gaagctgccc ggattgacgg tgccgggccg atgcggtttt tcaaggacat
     1921 tctggtgccg ctatcgaaaa cctcgattgc cgcgttgttt gtgattcagt tcatctacgg
     1981 ctggaaccaa tatttgtggc cgctgctggt gaccaccagc gaggacatgt acccggtagt
     2041 gattggcatc aagcgcatga tttccggcgg tgactccgag atgcagtgga atattgtcat
     2101 ggccactgca gtgctggcga tgctaccgcc agcgctggtg gtgatgctga tgcagaaatg
     2161 gtttgtgaag gggttggttg ataccgaaaa ataactgatt ggcatcccga tctgacgctc
     2221 ggggcgggtc atgcaaccgg tgatgctttg acgcggcggg atcgactcgc accggtatga
     2281 cacattcatc gatgccaaat ggcttgcatc aagttcaggt gggtgccagc agaccggcgg
     2341 ctgaagtcaa tttgtcacac ccctgacaca ttcaggtctt aaattcaccc caagcagccg
     2401 acaagcctcg gttttgaccc ccatttctca aaggacactt tcatgacgac atacaacatc
     2461 aaagcc
//
LOCUS       DAOSLL010000663         2459 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_36994_length_2459_cov_3.130616, whole genome shotgun sequence.
ACCESSION   DAOSLL010000663 DAOSLL010000000
VERSION     DAOSLL010000663.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2459)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36994_length_2459_cov_3.130616"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="PLB25_20555"
     CDS             complement(<1..756)
                     /locus_tag="PLB25_20555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE43997.1"
                     /translation="MRTDDSTPHVPLSAATLADTPPTLLFVDDEPSILSSLQRLFRPK
                     GYKILTAESGDAGLAILDAQPVDLVISDMRMPKMDGAQFLEKVREKTPETMRLLLTGY
                     ADVTSTINAINKGEIYRHISKPWDDNDIVLIVKKALEHKALRAENQRLLVLTQQQNDE
                     LKDLNSGLEKRVQERTAEIEQINSFLNLANGKLKQNFLVSIKVFSGLIELREGAVAGH
                     ARRVADMARRIASRMTLPTQVQQDVFVAALLHDI"
     gene            complement(805..1320)
                     /locus_tag="PLB25_20560"
     CDS             complement(805..1320)
                     /locus_tag="PLB25_20560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE43998.1"
                     /translation="MDSISSLHPLAKAWLLDGPLATHLDAYTALLERGNYAASTMQTS
                     FRALAHFAHWMSRCRVTAAQLNQVFLNLLVNAANAIETRGDITITTQSPTPGTIRISI
                     SDTGCGIPSLNQGRIFDPFFTTKSVGQGTGLGLSIVYCIVRKHYGNIEVESTVGQGST
                     FVLTLPVDPPA"
     gene            complement(1731..>2459)
                     /locus_tag="PLB25_20565"
     CDS             complement(1731..>2459)
                     /locus_tag="PLB25_20565"
                     /inference="COORDINATES: protein motif:HMM:NF013767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HOE43999.1"
                     /translation="LPLRTARAPVGTVGLRDGPTAVIRPDTQAPPALRPITTAALARL
                     AGVPGMNVARGLAALRGNSEKYLKLLVQFLELHADDMTRLTACRVASDHATALHLAHT
                     LKGTGATLGADQIAAPAAELEALLRANPIKGIPSDAITSRIDVIDLEFAVLATQLQSW
                     AAEPESAGLMLEDASAVARVLDELDRLLGKGDTEAISLFEAHEASLRTVLGVPCEELG
                     RQIRRFGLMSAQDTLHKLRQQNMT"
BASE COUNT          508 a          667 c          716 g          568 t
ORIGIN      
        1 gatgtcgtgc aacagtgcgg cgacaaatac gtcctgctgt acctgcgtgg gcaacgtcat
       61 acgactggca attcggcgtg ccatatcggc gacccggcgc gcatggccag caactgcacc
      121 ctcgcgcagt tcgatcaggc ccgagaaaac cttgatggaa accagaaagt tctgcttcaa
      181 cttgccattg gccaaattga gaaagctgtt gatttgttca atttcagcgg ttcgctcctg
      241 gacccgcttt tccagtccgg aattgaggtc tttgagctcg tcgttttgct gctgtgtgag
      301 gacgagcagg cgctggtttt cagcgcgcaa tgccttatgc tccaaagcct ttttgacgat
      361 caggacaatg tcgttgtcgt cccagggttt ggagatgtgg cgataaattt cacctttgtt
      421 gatggcattg atggtggatg tgacatcggc ataaccggtc aacagcaggc gcatggtttc
      481 aggggtcttt tctcgcacct tttcaagaaa ctgcgcgcca tccattttgg gcatgcgcat
      541 gtccgagatg accagatcaa cgggctgggc gtcaagaata gccaaaccgg cgtcaccact
      601 ctcagcggtg agtattttgt agcccttagg tcgaaacagt ctttgcagcg aactcaggat
      661 tgacggctcg tcgtccacaa acagcagggt gggcggggtg tcagccagag tcgccgcact
      721 aagtggcaca tgaggcgttg agtcgtcggt gcgcatgtat cggtacctgt ttttttttgg
      781 agacactcga gttgggaaac acgttcaagc agggggatca acgggcaatg tcagcacaaa
      841 agtggaaccc tgaccaactg tactctcaac ttcaatattt ccgtagtgtt tgcgaactat
      901 gcagtaaaca atggacaggc ccagtcccgt gccttgcccg acggatttgg tggtgaaaaa
      961 agggtcgaag atgcgcccct ggttcaagct ggggataccg caaccagtgt cggaaatgct
     1021 gattcggatg gttccgggag taggactctg ggttgtgatg gtgatgtcgc cacgggtctc
     1081 aatggcgttt gctgcgttga ccagcaaatt caggaatacc tggttgagtt gcgcggcggt
     1141 gactcgacag cgactcatcc aatgcgcgaa gtgcgccaaa gcacggaaac tggtctgcat
     1201 tgtgctggcc gcgtaattgc cgcgttcgag taacgccgta tatgcgtcga ggtgggtggc
     1261 aagggggccg tcaagtagcc aggccttggc aaggggatgc agcgagctga tcgaatccat
     1321 attggtcttc agttgtgaag ccactaccca tgcgcctatc ctgtaagtat gtaaagctca
     1381 ttcaccttcc aaacgcagtt tctccaagag aaaaagtcaa tcgcgtcgac agctatacat
     1441 cactgagccc tttgcataat ggccagcgac tgccattgcc gtattagatt gtgcctagca
     1501 gtcatcagtt gcaaaaaaca gagtgtcatg gagatcgtac tatgcgcggt tgaggactgg
     1561 cggtgatcct tgcccgtgtt ttggcatgcc aattggatgg accgggttgg tcggcatcca
     1621 cccttcctga tcgggttgac tctgccgaca ggcttcagtc aacatcactc ctgtcgaacg
     1681 tccaagcctc cgcaaaatcg cgacggccag gccaccttcg cacaattctc tcaagtcata
     1741 ttctgttggc gaagtttgtg caaggtgtcc tgcgcactca ttagcccaaa acgcctgatt
     1801 tgtcgcccaa gctcttcgca gggcacgccc aaaaccgtgc gcagggatgc ttcatgcgcc
     1861 tcaaaaagac tgatcgcttc ggtatcgcct ttgcccagca ggcggtcaag ttcgtcgagc
     1921 acccgcgcca ccgctgaggc atcctccagc atgagtcctg ccgattctgg ctctgctgcc
     1981 caagactgca attgtgttgc aagcaccgcg aactcaaggt cgatcacgtc tattctggat
     2041 gtgatcgcat cgctgggaat gcctttgatc gggttggcac gcagcagggc ttccagctcg
     2101 gctgccgggg ctgcgatctg atcggcaccc agcgtcgcgc ccgtcccctt gagcgtatgc
     2161 gccaggtgca gcgccgtcgc gtggtcgctg gccaccctac aggcggtcaa tctggtcatg
     2221 tcgtcggcgt gcagttccaa aaactgtacc aggagtttca ggtatttctc gctgttgccg
     2281 cgtagcgctg ccagcccgcg tgccacattc atgcccggca cgccagccaa tcgcgccagg
     2341 gcggctgtgg tgattggtcg cagggcaggt ggtgcctggg tatcgggccg gatgaccgcc
     2401 gttggaccgt cgcgtaaccc gactgtgcca actggcgcgc gggccgtgcg gaggggcaa
//
LOCUS       DAOSLL010000664         2455 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_37064_length_2455_cov_4.090417, whole genome shotgun sequence.
ACCESSION   DAOSLL010000664 DAOSLL010000000
VERSION     DAOSLL010000664.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37064_length_2455_cov_4.090417"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="PLB25_20570"
     CDS             <1..198
                     /locus_tag="PLB25_20570"
                     /inference="COORDINATES: protein motif:HMM:NF015129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44000.1"
                     /translation="RDALLFDLYRPQPTDAQADRQPRKSMAVRLTFNAEATAPTESQI
                     ESAVASVLATLVVQLGACQRT"
     gene            240..581
                     /locus_tag="PLB25_20575"
     CDS             240..581
                     /locus_tag="PLB25_20575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007870738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HOE44001.1"
                     /translation="MDLTVESLETPALTKAQLSDLLFDQIGLNKRESKDMIDAFFDLV
                     VHSLVDGQDVKITGFGNFQIRTKASRPGRNPRTGEAIPIDARRVVTFHASHKLKEQIQ
                     DQGPLSSRLLP"
     gene            678..1127
                     /locus_tag="PLB25_20580"
     CDS             678..1127
                     /locus_tag="PLB25_20580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HOE44002.1"
                     /translation="MENSLPSIPAKRYFTIGEVGDLCGVKPHVLRYWEQEFTQLRPMK
                     RRGNRRYYQHHEVLMIRRIRDLLYDQGFTISGARNKMEELLHAEHDQVSCGENLLDGL
                     EKIDVSYAKFDDFVDSEPLRALDLVPNPWQQVRLELCEIRDLLLSER"
     gene            complement(1272..>2455)
                     /gene="glnS"
                     /locus_tag="PLB25_20585"
     CDS             complement(1272..>2455)
                     /gene="glnS"
                     /locus_tag="PLB25_20585"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HOE44003.1"
                     /translation="RAKIDMASPNINMRDPAIYRIRRATHHNTGDKWCIYPMYTFAHP
                     IEDALEQITHSICTLEFEDQRPFYDWLLERLIEGGLLADPPPRQYEFARLNLTYVITS
                     KRRLAQLVTEHRVNGWDDPRMPTIVGLRRRGYTPQSLQNFAERIGVTKSDSWIDYSTL
                     EGCLREDLELKAHRGMAVLNPVKLVLTNWDELMGAGHLEPCTQPALPNPPEGVVSPVR
                     HFSVGKEVWIEREDFCEVPPKGYRRLFPGNKVRLKGGFVIACTGCVKAADGGISEVLA
                     TVVPDTKSGTPGSDQVKVKAAITWVGVANGINAEVRMYDRLFLDAQPDAGGKDFLESL
                     NPNSLKVVTAIVEPSLANALPGENFQFERHGYFVADRVDHTTSKPVFNLAVGLKDSWG
                     K"
BASE COUNT          523 a          678 c          677 g          577 t
ORIGIN      
        1 cgcgacgcgt tgctgtttga tctctatcga ccccaaccaa ctgatgcaca agcagaccgg
       61 caaccccgca agagcatggc ggttcgcttg accttcaatg ccgaggcgac cgccccgacc
      121 gagtcacaaa tcgaatcggc agtcgcttcg gtgcttgcta cactggtagt tcagcttggt
      181 gcctgccagc gaacctgacc ggatgccaaa tcgcgccgca taaatctaaa agagacctca
      241 tggaccttac tgttgaatcc ctcgaaaccc cggccttgac caaggcacag ttgtctgact
      301 tgttgtttga tcaaattggc ttgaacaagc gcgagtccaa agacatgatc gatgcattct
      361 ttgatttggt ggttcacagc ctggtcgacg gtcaggatgt caagatcact ggctttggca
      421 attttcagat tcgcaccaag gcatcccggc cagggcgtaa cccacgaacg ggagaggcaa
      481 ttccgatcga tgcgcgccgg gttgtgacct ttcacgccag tcataaactc aaggaacaga
      541 ttcaggatca gggacctctg tcgtcccgac tgttgccttg agttcgaaac aatccaaatt
      601 tatgtttcag accgccttta caggctgtat cgacatagag taacctgcca gcttttgggc
      661 atgtttcatt gaattcaatg gagaattctc tcccttccat accggccaag cgctatttca
      721 ccatcggtga agttggcgac ttgtgtggtg tcaaaccgca tgtgcttcga tattgggagc
      781 aggagtttac acagttgcga ccgatgaagc ggcgcggtaa ccgtcgctac taccaacatc
      841 acgaggtgtt gatgatccgg cgcatacgtg acctgcttta tgaccaagga tttaccatca
      901 gtggtgcgcg caacaaaatg gaggaattgc tgcatgctga gcatgaccag gtatcctgcg
      961 gcgagaattt gctagatggc ttggagaaga ttgatgtcag ctatgcaaaa ttcgatgatt
     1021 ttgttgattc cgagccactg cgggctcttg atttggtgcc aaacccgtgg caacaagtgc
     1081 gcttggaact gtgcgaaatt cgtgacctgc tgttgtccga acgatagtgc ctgtttgccg
     1141 aggttggctg tcagttgaag gagatccacg cggccacccg gtagcccctc gactgattgt
     1201 tgcgcggtcg gcactgtcac caatcgatgg ttggctttgg tgccaaaagc ccgccagcag
     1261 cgccaattac cctacttgcc ccagctatct ttcaaaccca ccgccaaatt gaataccggt
     1321 ttgctcgtgg tgtgatccac ccggtctgca acaaagtaac catgtcgctc aaactgaaag
     1381 ttttcaccgg gcagtgcatt cgccagtgac ggctcaacga tggcggtcac aactttcagg
     1441 ctgtttgggt tcaggctttc cagaaaatcc ttgcctccgg catcgggctg ggcatccaga
     1501 aacagtcggt cgtacatgcg tacctcggcg ttgataccgt tagccacacc gacccaggta
     1561 attgcggcct tgaccttcac ctggtcgctg ccaggggtgc cgctcttggt atcgggcacc
     1621 acggtggcca gcacctcgga aatgccaccg tccgcagctt tgacacagcc ggtgcaagcg
     1681 atcacaaagc cacctttcag tcgcactttg ttgccaggga acaggcgccg gtagcccttg
     1741 gggggcactt cgcagaaatc ttcccgttcg atccagacct ctttgccgac gctgaagtga
     1801 cgcacgggcg aaacgacacc ctctggaggg ttgggcaggg caggctgggt gcagggttcg
     1861 aggtggcctg cgcccatcag ttcgtcccag ttggtcagta ccagtttcac tgggttaagt
     1921 acggccatgc cgcgatgtgc tttgagttcc aggtcttctc tcaggcagcc ctcgagcgtg
     1981 ctgtagtcaa tccagctatc gcttttggtt acaccaatac gttccgcaaa attttgcagg
     2041 gactgcggtg tatagccccg ccgacgcagg ccaacgatgg tcggcatgcg tgggtcgtcc
     2101 cagccattga ctctgtgctc tgtcaccagt tgcgccagtc tgcgcttgct ggtgatcaca
     2161 taggtcaggt tcaaccgggc aaattcatac tggcgtggcg gagggtcggc cagcaggcca
     2221 ccttcaatca gccgctccag cagccaatcg tagaacggcc gttggtcttc aaactcgaga
     2281 gtgcagatgc tatgggtgat ctgctccagc gcatcctcga tcgggtgggc aaatgtgtac
     2341 atcggataaa tgcaccattt gtccccggta ttgtgatgag tggcacggcg gatacggtaa
     2401 atggccgggt cgcgcatgtt gatgttgggg ctggccatgt cgatcttcgc gcgca
//
LOCUS       DAOSLL010000665         2443 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_37284_length_2443_cov_4.466080, whole genome shotgun sequence.
ACCESSION   DAOSLL010000665 DAOSLL010000000
VERSION     DAOSLL010000665.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37284_length_2443_cov_4.466080"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(634..1482)
                     /locus_tag="PLB25_20590"
     CDS             complement(634..1482)
                     /locus_tag="PLB25_20590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HOE44004.1"
                     /translation="MTDLIDTIARSLARAAVIPYLGPDMLSLCSDLQVPGTPLALAEY
                     MTAKVSVPHKIRKRLTQAAQFIENFKHRKSVVHLMNQAFASTPAPSSLHLALAASGAG
                     LWVDTWYDDTFASALAQTRPSDDWVQVQGLSQSEHFGQWTGAYTADGTPLAALSGEPG
                     RVLYKPIGSHGPAGNYLVSDSDFVEVLTEIDIQTPIPAAVQAWRIGRHFLFLGCRFDD
                     QLTRCFARQIMKRSGDRHWAVLPNPPTRMEARFLQEQNITRIDMPLAQFAESLLMSQS
                     SMLPTD"
     gene            complement(1679..1939)
                     /locus_tag="PLB25_20595"
     CDS             complement(1679..1939)
                     /locus_tag="PLB25_20595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44005.1"
                     /translation="MAITPDDLRAAGAVIDAAGSVREAAATWRTRDPAMRVLVVDAHD
                     MRDETPALRLGMRSVYLATSNGHCWSVTAQPELATALILTQY"
     gene            complement(1942..2166)
                     /gene="nifT"
                     /locus_tag="PLB25_20600"
     CDS             complement(1942..2166)
                     /gene="nifT"
                     /locus_tag="PLB25_20600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative nitrogen fixation protein NifT"
                     /protein_id="HOE44006.1"
                     /translation="MKLMIRKDSKGVLTGYVAKKDLEEPIVAMANPDMWGGLVTLANG
                     WQFDLPAMPIDTPLPITVEARRMALTEAGD"
     gene            complement(2210..>2443)
                     /locus_tag="PLB25_20605"
     CDS             complement(2210..>2443)
                     /locus_tag="PLB25_20605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HOE44007.1"
                     /translation="QRLQRLGVAASSGAACSSEGSKPSHVLTAMGVSADEALAAVRLS
                     LGRSSTQAEVSSLLNTLPALLQPLLREELMTAP"
BASE COUNT          441 a          739 c          773 g          490 t
ORIGIN      
        1 cggcgcctat ttgaatttgt aggagtcgcg ccctcgcgac gaatgacttg gaatgcttga
       61 attcttcgca tgatatgcaa agacttgact tgttaaagac tatgcagatg ttgagtcagc
      121 cggcctcaat cgggcggttg actggcaacc cgcgcatcag agtgtgccca gttcaaactc
      181 gatggcgctg acaattgctg ggccgcagcg ctggaccaac cattgacagc ttttccgcac
      241 cgcatctaac agactgaatc tgccattgct cggcacaggc aagcaacggt ttgattttgg
      301 gaaccaggct gttttgcgcg gcctggaggc ggatgtggtc gcatggtcgc cttttgcgct
      361 gactgccttg tcatgatccg ggcctgctaa gcacttcaag gcgtgagcgg caggacgact
      421 ttgtggccgg atgccatttg cgccaccatg cgctcaatgc aaaaaaccgg gccaagccgc
      481 cattgggcgc agacttcaac atcgccaacc ggcccttcgg cactatttaa atagtagcta
      541 ccagcgcata ttccacgagg gctacaggcc tatttcgctt gaagttcaag gggcagactg
      601 gcgctgcaat accagcgtct ggaatgatgc aggctaatcg gttggcaaca tgcttgactg
      661 gctcatcaag agagactcgg caaactgcgc cagcggcatg tcgatgcggg tgatgttttg
      721 ctcttgcaga aagcgcgctt ccatgcgggt tggtgggttg ggcagcacgg cccaatggcg
      781 gtcgcccgag cgcttcatga tctggcgggc gaagcagcgc gtcaactggt catcaaaccg
      841 gcaacccaga aacagaaaat ggcggccaat ccgccaggct tgcaccgctg ccggaatcgg
      901 ggtctgaatg tcgatttcgg tcagcacttc aacaaagtcg ctgtcagaca ctaggtagtt
      961 gcccgccggg ccgtggctgc cgatgggctt gtaaagcacg cgtccgggtt cgccagacag
     1021 tgctgccagg ggcgtgccgt cggcagtgta ggcaccggtc cactggccaa aatgctccga
     1081 ctgcgacaag ccctgcacct gcacccagtc atcgctcggc cgggtctgcg ctagggcgct
     1141 ggcaaaggtg tcgtcgtacc aggtgtccac ccacagcccg gcaccactgg cggccagcgc
     1201 cagatgcagg cttgacggcg ccggggtgct ggcaaacgcc tggttcatca gatgcaccac
     1261 cgacttgcgg tgtttgaagt tttcgatgaa ctgcgcggct tgcgtcaggc gtttgcggat
     1321 cttatgcggc acgctcacct tggccgtcat gtactcggcc aacgccagtg gtgtgccagg
     1381 cacctgcaag tcgctgcaca gtgacagcat gtctggcccc aggtagggaa tcaccgcagc
     1441 tctggcaagg ctgcgggcaa tggtatcgat caggtcggtc atggttttcc ttgcgaaggt
     1501 gaatgaggct gcaagcccgg gggtacttgt cgtctggcca gggtgccggt caagctttgg
     1561 gcatgtcgat agcgccaacc gaccccggcg aggccggttt gcgccgggct tgtcggccca
     1621 tcgcccctct atggttgcgg tcggctggca gccgctgcgc catcagtgcg accggcggct
     1681 agtactgcgt caggatcagc gccgtggcca gctcaggctg ggcggtcacg ctccagcagt
     1741 ggccgttcga ggtcgccagg taaacgctgc gcatgcccag gcgcagcgcc ggggtttcgt
     1801 cgcgcatgtc gtgcgcgtca accaccagca cacgcatcgc cgggtctcgg gtgcgccagg
     1861 tggccgccgc ctcgcgtacc gaaccggcgg cgtcaatcac cgcgccagca gcgcgcagat
     1921 cgtcgggggt aatggccatg ctcagtctcc tgcttcggtc agcgccatgc ggcgcgcttc
     1981 gaccgtgatc ggcagcggtg tatcgatagg catggcaggc aggtcgaact gccagccatt
     2041 ggccagcgtc accagcccgc cccacatgtc cgggttggcc atcgcgacga tgggttcctc
     2101 caggtctttt ttggcaacat agccggtcag cacgcctttg ctgtctttgc ggatcatcaa
     2161 tttcatcatc agctcctggt gggttggaaa tgggggccat tggtttgctt taaggagccg
     2221 tcatcaattc ttcgcgcagc aggggctgca gcagcgcggg caaggtgttc agcaggctgc
     2281 tcacttctgc ctgggtgctg ctgcggccca gcgacaggcg caccgcggcc agcgcctcat
     2341 cggcgcttac acccatcgcc gtgagcacat gcgagggctt gctgccctcc gatgagcagg
     2401 ccgctccgct ggaggccgcc acacccaggc gctgcaggcg ctg
//
LOCUS       DAOSLL010000666         2422 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_37724_length_2422_cov_2.817490, whole genome shotgun sequence.
ACCESSION   DAOSLL010000666 DAOSLL010000000
VERSION     DAOSLL010000666.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37724_length_2422_cov_2.817490"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1398
                     /gene="leuC"
                     /locus_tag="PLB25_20610"
     CDS             <1..1398
                     /gene="leuC"
                     /locus_tag="PLB25_20610"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007866636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HOE44008.1"
                     /translation="EEDGTALLYIDRHLVHEVTSPQAFEGLRQASRKVWRVSSIVATA
                     DHNTPTTGWERGYDGITDPTSKEQVLTLDANIREFGSAAYFPFLSKRQGIVHVIGPEN
                     GATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHVMATQTLLAKKAKNMLVRVDGK
                     LPPGCAGKDIVLAVIGKIGTAGGTGYTIEFGGEAIRALSMEARMTVCNMAIEAGARAG
                     LVAVDDKTIHYVKGRPLAPGNPASTEAAAAVEWDQAVTYWKTLHSDAGAHFDTVVEID
                     ASALVPQVTWGTSPEMVLGIDARVPDPDKEKDVNKRGAIERALTYMGLQPGKALNDLY
                     VDKVFIGSCTNSRIEDMREAAAMVKTLGRKVASNIRLAMVVPGSGLVKEQAEREGLHE
                     VFKAAGFEWREPGCSMCLAMNADRLEPGERCASTSNRNFEGRQGAGGRTHLVSPAMAA
                     AAAVYGHFVDIRKFV"
     gene            1417..1551
                     /locus_tag="PLB25_20615"
     CDS             1417..1551
                     /locus_tag="PLB25_20615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009855913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="entericidin A/B family lipoprotein"
                     /protein_id="HOE44009.1"
                     /translation="MKKSSFCVLLIASLLTLTGCNTVKGVGQDLQKAGATIENAAKKN
                     "
     gene            1561..2214
                     /gene="leuD"
                     /locus_tag="PLB25_20620"
     CDS             1561..2214
                     /gene="leuD"
                     /locus_tag="PLB25_20620"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HOE44010.1"
                     /translation="MQRFSILQGLVAPMDRENVDTDAIIPKQFLKSIRKTGFGENLFD
                     EWRYIDAGYPGQDPASRKPNPDFVLNQARFKGASILLARKNFGCGSSREHAPWALDQF
                     GFRAIIAPSYADIFFNNSFKNGLLPIVLSESQVGQLFDEVAAFPGYTLTVDLERQVVV
                     KPNGDELPFEVQAFRKYCLLNGFDDIGLTLRHADKIKAFEAQRLAQKPWLARTMISS"
     gene            2305..>2422
                     /locus_tag="PLB25_20625"
     CDS             2305..>2422
                     /locus_tag="PLB25_20625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019703708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HOE44011.1"
                     /translation="MKIAVLPGDGIGTEIVAEAVKVLQALALPFEMETALVGG"
BASE COUNT          559 a          678 c          682 g          503 t
ORIGIN      
        1 gaagaagacg gtactgccct gctctacatt gaccgccacc tggtgcacga ggtcactagc
       61 ccccaggcct tcgagggatt gcgccaagcc agtcgcaaag tctggcgtgt gagttcaatt
      121 gtggccaccg cagatcacaa tacacccacc acggggtggg agcgcggtta tgacggcatc
      181 acagacccca ccagcaaaga acaggtattg acgctggacg caaacattcg ggagtttggt
      241 tctgccgcgt atttcccttt cctgtccaaa cgccaaggaa ttgtgcatgt gatcggccct
      301 gagaatggcg ccaccttgcc gggcatgacg gttgtctgcg gtgactcgca cacgtcgacc
      361 cacggtgcat ttggcgcgct ggcccacggc attggcacca gtgaagttga gcatgtcatg
      421 gccacccaga ccttgctcgc caaaaaagcc aaaaacatgc tggttcgcgt ggacggaaag
      481 ctgccgcctg gctgcgcagg caaagacatc gtgctggcag tcattggaaa gatcggcacg
      541 gcaggtggca cgggttacac catcgagttt ggtggcgaag ccatcagggc gctcagcatg
      601 gaagctcgca tgaccgtctg caacatggcc atcgaagccg gcgcccgcgc tggcttggtg
      661 gctgtggatg acaagacgat ccactatgtc aagggccggc ccctggcccc cggcaaccct
      721 gcctccaccg aggctgcagc tgctgtcgaa tgggatcaag ccgtgaccta ctggaagaca
      781 ctgcattccg acgctggtgc gcattttgac actgtggtcg agatcgacgc atcggcgctg
      841 gtgcctcagg tcacctgggg cacgtctccg gaaatggtgc tgggcattga cgcacgcgta
      901 cctgatcccg acaaggaaaa ggatgtcaac aaacgaggtg cgatagaacg tgcactgacc
      961 tacatggggc tgcagcctgg caaggcgctc aatgacctgt atgtggataa agtgttcatc
     1021 ggttcatgca ccaacagccg catcgaggat atgcgcgagg ctgctgccat ggtcaaaacc
     1081 ctgggccgca aggtggcatc caacattcga cttgccatgg tggttcccgg ttctggcctg
     1141 gtcaaggaac aagctgagcg agaaggtctg cacgaagttt tcaaggctgc aggctttgaa
     1201 tggcgagagc ccggctgctc aatgtgcctg gcaatgaatg ccgacaggct cgaacccggt
     1261 gaacgctgtg cctccacgag caaccgcaac tttgaaggtc gccagggagc cggtggtcgc
     1321 acacatctgg taagcccagc catggctgct gctgctgcag tgtatggcca ctttgtcgac
     1381 atccgaaagt tcgtttgagg tgacctgaaa gacgccatga agaaatcatc attctgcgtg
     1441 ttgttgatcg ccagtctatt aacactcact ggctgcaata cggtcaaagg tgtggggcaa
     1501 gacctgcaga aagctggtgc gacgatcgaa aacgcagcca aaaagaatta ggaacttgtg
     1561 atgcaaagat tttcaatact ccaaggtctc gtggccccga tggatcggga aaacgttgac
     1621 accgatgcca tcattccaaa gcagtttctg aagtccatcc gcaaaaccgg attcggggaa
     1681 aacctgtttg atgaatggcg ctacattgat gcgggctacc ccggccaaga ccccgccagc
     1741 cgcaaaccca atcccgactt cgtgctcaat caggcacgct tcaagggtgc ttccatcttg
     1801 cttgcgcgca agaacttcgg ttgtggctcc tcacgtgagc atgcgccgtg ggcgcttgat
     1861 cagtttggct ttcgcgccat cattgcacca agctacgccg acattttctt caacaacagc
     1921 ttcaagaatg gcctgctgcc cattgtgctg agtgaatcgc aagttggtca actgtttgac
     1981 gaagtagctg cttttccagg ctacacactg accgtggacc tggaacgaca ggtggttgtc
     2041 aaacccaatg gtgacgagtt gccttttgag gtgcaggcat ttcgcaagta ttgtctcctc
     2101 aatggatttg acgacatcgg tttgacgctg cgccatgcag acaagatcaa agcctttgag
     2161 gcccagcgac tggcgcaaaa accctggcta gcccgcacca tgatttcttc ataagctact
     2221 gtaaacatag caatacactc cctcaaaagg cacggattgc ccacccgctt cctgcctgcc
     2281 ctgctcctga tacgaaagta aacgatgaaa attgcagtct tgcccggcga cggcattggt
     2341 accgaaattg tggctgaagc agtcaaggtg ctgcaggctc ttgcgctccc attcgaaatg
     2401 gaaaccgctc tggtaggcgg cg
//
LOCUS       DAOSLL010000667         2404 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_38104_length_2404_cov_3.896977, whole genome shotgun sequence.
ACCESSION   DAOSLL010000667 DAOSLL010000000
VERSION     DAOSLL010000667.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2404
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38104_length_2404_cov_3.896977"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="PLB25_20630"
     CDS             <1..443
                     /locus_tag="PLB25_20630"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015602282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HOE44012.1"
                     /translation="QRQYPEITDFYLPPEGCSYRLAVVQIKKAYPGHARRVMFGIWSF
                     LRQFMYTKFIVVVDEDVNPRDWEDVIWAITTRVDPSRDTLLADSTPIDYLDFASPVSG
                     LGSKMGLDATNKWPGETSREWGRPLAMSAEVTARVELLWQSLGL"
     gene            648..929
                     /locus_tag="PLB25_20635"
     CDS             648..929
                     /locus_tag="PLB25_20635"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HOE44013.1"
                     /translation="MTVTVKLDDALECALRSRCKALGRSASALVRDALQACLTQTDPA
                     PALFYTLGQDLYGRHAGPVDLASQRKADLSHIWDQKHPARASAESDGSA"
     gene            1025..1171
                     /locus_tag="PLB25_20640"
     CDS             1025..1171
                     /locus_tag="PLB25_20640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44014.1"
                     /translation="MWLQTNPKAGLLTTWPVVAEVCAMLARRVTNAAALDFFALGATG
                     WIYA"
     gene            1187..1387
                     /locus_tag="PLB25_20645"
     CDS             1187..1387
                     /locus_tag="PLB25_20645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44015.1"
                     /translation="MLPETLAHVILIIERFSDLPFELADASVPEAASRHRIRHVLTID
                     SDFDIYRDSQGKALRHVLRLRG"
     gene            complement(1637..>2404)
                     /locus_tag="PLB25_20650"
     CDS             complement(1637..>2404)
                     /locus_tag="PLB25_20650"
                     /inference="COORDINATES: protein motif:HMM:NF038122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NF038122 family metalloprotease"
                     /protein_id="HOE44016.1"
                     /translation="GCAANGGLNTKGGTTHCPASYSKLINNTTDNSGISHLYAGITAL
                     EMTRANAKAIGLLGSSAAEDAAITFSSGFNFDFDSSDGIGAALYDFVGIAIHEIGHAL
                     GFTSGVDVLDTNGRNRFSQKQFDPFATVLDFTRCTDASEAAGADMDWTIGGVTRDFAI
                     NGSCSGAAKVSNAWSTGRILGDGYQASHWKDDLGRGIMDPTFASGEFGQISALDILAF
                     DVIGWDLQQSSSIPEPSGLLLLATALLGAAATRRRRA"
BASE COUNT          516 a          701 c          663 g          524 t
ORIGIN      
        1 tgcagcgcca gtaccccgaa atcaccgact tctatttgcc gcccgaaggc tgcagctacc
       61 ggctggcggt ggtgcagatc aagaaggcct accccggcca tgcgcggcgg gtgatgtttg
      121 gcatctggag ttttctgcgc cagttcatgt acaccaagtt catcgtggtg gtcgatgagg
      181 atgtcaaccc gcgcgactgg gaagacgtga tctgggccat caccacccgg gttgacccaa
      241 gccgcgacac cctgctggcc gacagcacgc cgattgacta cctcgacttt gcctcgccag
      301 tgagcggcct gggcagcaag atggggctgg atgcgaccaa caaatggccg ggtgaaacca
      361 gccgggaatg gggtcggcca ctggccatga gcgcggaagt cacggccagg gtcgagctgc
      421 tctggcaaag cctgggctta taagccaaat tggcctccag cccttatggc tattgcgtaa
      481 acagctattg tttatagagc aattcagttg aggagcaatc ctgctgccag gattgctgcc
      541 atgattccac cacccccccg ctcaatctgc gcgagaaata cccgctgtaa aaacatcctt
      601 ggtgcggatg caatcattct ggcagtaccc gaatggagtt caaccacatg accgtgacag
      661 tcaaactcga tgacgcactt gaatgtgcct tgcgcagccg ttgcaaggca cttggacgct
      721 cggccagtgc cttggtgcgt gatgccttgc aggcttgtct gacgcaaact gatcccgcgc
      781 cagcattgtt ttacacgctg ggacaggatt tgtatggaag acatgcaggt cctgttgatc
      841 tggccagtca acgcaaagcc gacctgtcgc acatttggga tcaaaagcac ccggcacgcg
      901 ccagcgccga atccgatggc agtgcctgaa gcccggtctg ggacggcccg cactgtggtg
      961 gccgacggcg gcccactgct gacactcttc aaccaagccg accattggca tcctgctgtt
     1021 ttgcttgtgg ctgcaaacca acccgaaagc tggcctgctg accacttggc cggtagttgc
     1081 cgaagtttgt gccatgctgg cacgacgcgt gaccaatgcc gccgcgctgg acttttttgc
     1141 gctgggggcc acggggtgga tttacgcttg atgccccggc agcgacatgc tgcccgagac
     1201 cctggcgcac gtcattttga tcattgaacg cttttcagat ttgccgtttg aactagccga
     1261 tgcctcggta cccgaagccg catcacgcca ccggattcga catgtgctga cgattgactc
     1321 ggactttgac atttaccgcg acagccaggg caaagcgttg cgccatgtcc tgcgattgcg
     1381 aggctaaagg ccgatcagcg catcaactgg tgatcggtca aagaggcgat ccagatacgc
     1441 tgcgcggcag ttctgcccga gtcatacccg ttgcaccgga aagcaccctc caacacagag
     1501 cttctggtgc taaccgtacc atgcaaggca acaaatcgag ggtcttcaag cagttcaagg
     1561 gtgctccacg gtgcaatatc tggcctcgtg tgattgaaaa gcttatccgt tttcgtccgt
     1621 ccgtatccgt cgtccatcag gcccgacgac gacgggtagc ggctgcaccc aacagcgctg
     1681 tcgccaacaa caacaagcct gacggctccg gtatgctgga ggactgttgc aagtcccaac
     1741 cgatcacgtc gaaagccaaa atatcaagtg cgctgatttg cccaaactcg ccacttgcaa
     1801 aagtgggatc catgatgccg cggcctagat cgtctttcca gtggcttgcc tgatagccgt
     1861 cacctaagat tctccctgtt gaccaggcat tgctgacttt ggctgcacca gagcaacttc
     1921 cgttgatggc gaagtctctc gtcacgccac caatcgtcca atccatgtct gcgccagcag
     1981 cttcgcttgc atctgtacaa cgagtgaaat cgaggacagt tgcgaaaggg tcgaattgtt
     2041 tttgggaaaa cctgtttctg ccgttggtgt caagtacgtc aacaccactg gtgaagccca
     2101 gggcatggcc tatttcgtgg atagcgatgc cgacgaagtc gtacagggca gccccaatgc
     2161 catcggatga gtcaaagtcg aaattgaacc cactgctaaa ggtaattgct gcatcttcgg
     2221 cagcgctact gcccaagagc ccaattgctt tggcattggc acgtgtcatt tccaaggcag
     2281 taatgccggc gtacagatga cttatgcccg agttgtcagt cgtgttatta atcagcttgc
     2341 tgtaacttgc tggacagtgc gtagttcccc ccttcgtgtt caggccgcca ttggctgcgc
     2401 atcc
//
LOCUS       DAOSLL010000668         2385 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_38458_length_2385_cov_4.553648, whole genome shotgun sequence.
ACCESSION   DAOSLL010000668 DAOSLL010000000
VERSION     DAOSLL010000668.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38458_length_2385_cov_4.553648"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="PLB25_20655"
     CDS             complement(<1..524)
                     /locus_tag="PLB25_20655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429920.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HOE44017.1"
                     /translation="MQAYYLVILQGSLLTVGVSLLALLVSILFGLGGAAAKLSGNPIL
                     VGLATFYTTIIRGIPDLVLMLLVFYGGTIGINTLLEKIGSEATIDIEPFAAGVLTIGF
                     IYGAYMTETFRGAILSIPKGQMEAAWAFGMGRFQTFVRITLPQMVRYALPGFTNIWLV
                     LIKATALVSLIGLQ"
     gene            complement(652..1419)
                     /locus_tag="PLB25_20660"
     CDS             complement(652..1419)
                     /locus_tag="PLB25_20660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HOE44018.1"
                     /translation="MKKLLLAVAIGALCATAFAQGKDLKVAIDATYEPFTFKTADGKP
                     TGFDVDIASALCEQIKRKCVFVEQAWDGMIPGLMAKKYDVIISSMSITEERLKQVDFT
                     DKYYNTPSKIVLKKDIKFSGIDSIKGKKVGVLKASTQEKYALGELKTKGVEVVSYDAQ
                     DQVYLDIKAGRLDGTVADTQEVMGGFLSKPGGESYQFVGDDLYIPKYFGIGAGIALHK
                     GQPALKAELNAAIKAIRGNGKYKAINDKYFKFDVYGK"
     gene            complement(1934..2212)
                     /locus_tag="PLB25_20665"
     CDS             complement(1934..2212)
                     /locus_tag="PLB25_20665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44019.1"
                     /translation="MANQTKTSTVTDPSVIDDRVLLLEEVDFKWLMAGHGWWIDTSRL
                     HCDSGYATELLRLAGATQSPTLRACARLLQKQVSMRCDRTLRSVELAD"
BASE COUNT          537 a          670 c          648 g          530 t
ORIGIN      
        1 tcctgcaggc caatcaagct caccagcgcg gtagccttga tcaaaaccag ccagatgttg
       61 gtgaaaccgg gcagtgcgta gcgcaccatt tggggcagcg tgatgcgcac aaaagtctga
      121 aatcggccca tgccaaaggc ccaggctgcc tccatttggc ccttggggat gctcaggatg
      181 gcgccgcgaa aggtctcggt catgtaggcg ccgtaaataa agccgatggt gagtacgcca
      241 gcggcaaacg gctcaatgtc gatggtggct tcgctgccga ttttttcaag cagcgtgtta
      301 atgccaatgg tgccaccata gaacaccagc agcatcagca ccaggtcggg aataccccga
      361 atgatggtgg tgtaaaaagt ggccaagccg accaaaatcg ggttgccaga gagcttggct
      421 gccgcaccac ccaggccaaa caggatggac accagcagtg ccagcaagga cacccccacc
      481 gtcagcaggg agccttgcaa gatgaccagg taataggctt gcatgctggt cacccgcgtg
      541 aacttgaccg atttgcaaac gcgaccaccc agcgccacgg atttccgcga tcacggcaat
      601 gacagggcgc tgcaggcagc atcaagcgac tcgctggcaa gtcaggatga attacttgcc
      661 gtaaacgtca aacttgaagt atttgtcgtt gatggccttg tatttgccat tgccgcgaat
      721 cgccttgatg gcagcgttaa gttctgcctt cagcgcgggc tgacccttgt gcaaggcaat
      781 gccagctcca atgccaaagt atttcggaat gtacaagtca tcgccgacaa actgataact
      841 ttcaccaccg ggtttgctaa ggaagccgcc catcacttcc tgggtatcgg ctaccgtacc
      901 gtccagacgg cctgccttga tgtcgagata aacctggtct tgtgcgtcgt aggagaccac
      961 ttcgacaccc ttggtcttga gctcgccaag cgcgtatttt tcctgggtgc tggccttcaa
     1021 gacaccaacc ttcttaccct tgatcgaatc aatgccgctg aatttgatgt ctttcttcag
     1081 cacgatcttg ctgggcgtgt tgtagtactt gtcggtgaag tcaacttgct tgaggcgctc
     1141 ttcggtgatc gacatcgaac tgatgatgac atcgtatttc ttggccatca ggcccggaat
     1201 catgccgtcc caggcctgct cgacaaacac gcacttgcgc ttgatctgtt cgcaaagggc
     1261 gctggcaatg tccacgtcaa aaccggtggg cttgccgtca gccgtcttga aggtaaacgg
     1321 ctcataagtg gcatcgatgg caactttgag gtctttgccc tgggcaaaag cagtggcaca
     1381 cagcgcacca atggcaacag ctagcagaag tttcttcatg aagtgtcctc aaaaaatgac
     1441 gggcagtgcc cggcgggtta acaaaaaatt aaaaactgat tctacggtca gtcgtttgtc
     1501 tgggcggggc tggtttctac tgagcgcgtg tcatattcag ccaaagcaat catcatgccc
     1561 ggctgattca tcacggctcg cgtttggcgc gagacaacca gatctgctct ggagagcatc
     1621 tgcttgctcg acaacgcgca acctcaaatc cgccgctctt cgtgcccaga ttggtctttc
     1681 cgcctggtca cccaagactg ctgccgttct gattgcgcct gccttacccg accttgcgca
     1741 aatctgccgt ggaacctgga cgatttgcgt cagatcgaac ccgaggggtt acatgcggaa
     1801 atccgcgagg caggcgaatc cgctggtggt tctatccagg aaacgcctgt cggacgtaag
     1861 gcatcgaaca gcgagagcca accccggcgc agtgggaaat ggcaccagct ttggctcgca
     1921 tccggcctaa atctcaatcg gcaagctcga cgcttcggag ggttcggtcg cagcgcatgc
     1981 tgacttgctt ttgcaatagg cgcgcacaag cgcgcaaggt cggagactgc gttgcccctg
     2041 ccagcctgag caactctgtc gcgtagcccg aatcgcaatg caggcgcgag gtgtcaatcc
     2101 accacccatg cccggccatg agccacttga aatcgacctc ttccaacagc aaaacccggt
     2161 catcgatcac agatgggtct gtcaccgttg aagtttttgt ctgatttgcc atgaagtcac
     2221 tctccttgca tgccggttgc gacatgcgtc tggttagttt gctggccaaa gtgtgcgtca
     2281 gtggtgctgc tgtcaagttt gaaaaagcgg cagaaacagg cgctgttttc gggcttgaac
     2341 aactttccga agcttacagg ctttgcagct tttccagtgc caggt
//
LOCUS       DAOSLL010000669         2382 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_38522_length_2382_cov_3.730984, whole genome shotgun sequence.
ACCESSION   DAOSLL010000669 DAOSLL010000000
VERSION     DAOSLL010000669.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38522_length_2382_cov_3.730984"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="PLB25_20670"
     CDS             complement(<1..417)
                     /locus_tag="PLB25_20670"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOE44020.1"
                     /translation="MNQSIAQNVTPYGTLPTTSSPASRKPVSLPRLLEMRARGEKIAM
                     LTAYDATFAAVADAAGVDCLLIGDSVGMVCQGLSSTVGVSLETMQYHVACVTRGVRRV
                     QGTVWIVADLPYGSYHESREQALRSAAVLMQAGAHMV"
     gene            complement(521..877)
                     /locus_tag="PLB25_20675"
     CDS             complement(521..877)
                     /locus_tag="PLB25_20675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44021.1"
                     /translation="MGQNAGAKCAAAAVGWQPMLHEFDGLTTLWRGGSRTCNISICDL
                     RLRWSGTKRSIRVVRSDAFVLLEQVQSISPYSLYSHLHDLSRIVKKTPYSLKKTRKKL
                     SFGKRPTPKKTQDQPG"
     gene            complement(1059..>2382)
                     /locus_tag="PLB25_20680"
     CDS             complement(1059..>2382)
                     /locus_tag="PLB25_20680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011803191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HOE44022.1"
                     /translation="TFGFELKPGLKFASGNAISAEDVVWSLQRAVLMDKGPAFILTQF
                     GFSKENVKDKIKQTGPLTLTIETDRAYAPTFVLNCLTANVAAVLDKKLVLSKAVAGDM
                     GGAWMKTNYAGSGAMKLREWRANELLVLERNDNYYADKAKLARVIYRHIKESATQRLL
                     LEKGDADIARNLSPQDLAAVSANKGILTTATPKGTVYYISLNQKNPILAKPEVREAFK
                     WLVDYSAIGDTIIKNIGVVHQNFLPTGLLGASSINPYKLDVAKAKALLTKAGYPNGFK
                     VSIDMRTMQPVQGITEAFQQTAKRAGIEVEIIPGDGKQVLTKYRARTHDLYIGQWGAD
                     YWDPHTNADTFVRNPDNADNAKAKPLAWRNAWDIPALTKKADAAVLERDPAKRKLMYE
                     EMQAEFRATSPFVMLYQQTEVAAFRTNVEGLRLGSTMDSTYVYRVAKR"
BASE COUNT          465 a          663 c          691 g          563 t
ORIGIN      
        1 gaccatgtga gcccccgcct gcatcagcac cgctgcgctg cgcaaggctt gctctctgga
       61 ttcgtggtag gagccatatg gcaggtcagc cacaatccag accgtgccct gtacccggcg
      121 cacgccacgg gtaacgcacg ccacatggta ttgcatggtc tctagtgaaa ccccgacggt
      181 gctcgacagg ccctggcaga ccatgcccac tgagtccccg atcaaaaggc aatcgacccc
      241 agccgcatcg gccactgccg caaaggttgc atcgtaggcg gtaagcatgg cgattttctc
      301 gccacgtgct cgcatctcca acaggcgcgg taggctgact ggtttgcgag aggccgggct
      361 gctggtcgtt ggcagcgttc catagggcgt gacattttgg gcaatggact gattcatgct
      421 aaaaatccga aaataagtgg cgcaagtcta gttgagttgg atcgtgatgc gttggtctat
      481 tgtctggtac ttgaaaccgc cgagttggaa cagcgtattt tcagccgggc tgatcttgcg
      541 tctttttggg ggtcggtctt tttccaaagg atagtttttt acgcgtcttc ttcaagctgt
      601 agggtgtttt cttcacaatt cgactgaggt cgtgcaggtg gctgtagagg ctgtaggggc
      661 tgatactctg gacctgttcg agcagcacaa atgcgtcaga tcggacaacg cggatcgatc
      721 tttttgtccc cgaccatcgc aaccggagat cgcagatgct gatgttgcaa gtccgactgc
      781 caccacgcca cagggtagtc aacccgtcaa attcatgcaa catcggctgc cagccaaccg
      841 cagcagcagc acattttgca ccggcatttt gacccatagc cgggctatgc tcctgcaaat
      901 ccggcccaaa ctggcctcac ttgggtcggc tcttgagtcg gctcatgctg cctgatcgac
      961 aagtgcgaaa ggcacctgcg tggtgctcat gcaattacgc gtcccgtcac tggccgatgc
     1021 cttctgaccg gaggcgactg cgttcaaaga cgatccgatc agcgcttggc cacccgatag
     1081 acgtaagtcg aatccatcgt cgaacccagg cgcaaaccct cgacgttggt gcgaaaagcc
     1141 gcaacttcag tttgctggta cagcatcaca aaggggctgg tggcgcggaa ctcggcctgc
     1201 atttcttcgt acatcagctt gcgtttggcc gggtcgcgct caagcactgc ggcatcggct
     1261 tttttggtca acgcgggaat gtcccaggcg ttgcgccagg ccagtggctt ggctttggca
     1321 ttgtcagcgt tgtcgggatt gcgcacaaaa gtatcggcat tggtgtgcgg atcccaatag
     1381 tcggcacccc actgaccaat gtaaaggtca tgggtgcggg cgcggtactt ggttagcact
     1441 tgcttgccgt cgccagggat gatttccacc tcaatgccgg cgcgtttggc ggtttgctga
     1501 aaagcctcgg taatgccttg caccggctgc atggtgcgca tgtcaatcga gaccttgaag
     1561 ccatttgggt aaccagcctt ggttagcagg gctttggctt tggccacatc aagcttatag
     1621 ggattgatac tgctggcacc gagcagaccc gtcggcaaaa aattctggtg aacaacacca
     1681 atgttcttga tgatggtgtc gccaatggct gaatagtcca ccagccattt gaaggcttca
     1741 cgcacctcgg gcttggccag aatcggattt ttctggttca ggctgatgta gtacaccgtg
     1801 cccttgggtg ttgcggtggt caagatgccc ttgtttgccg acaccgctgc aaggtcctgc
     1861 ggcgacaagt tgcgggcgat gtcggcatcg cccttttcca gcagcaggcg ctgggtggcg
     1921 gattccttga tgtggcggta gatcacgcgc gccagtttgg ccttgtcggc atagtagttg
     1981 tcgttgcgct caagcaccag cagctcgttg gcgcgccatt cgcgcagttt catggcaccg
     2041 gaaccggcgt agttggtttt catccaggcc ccgcccatgt caccagccac tgccttggac
     2101 agcaccagtt ttttgtccag cacggcggcc acgttggcgg tcaagcaatt gagcacaaag
     2161 gtcggtgcgt aggctctgtc ggtctctatc gtcaacgtta gggggccggt ttgtttgatc
     2221 ttgtccttga cgttctcttt gctaaagcca aattgcgtca ggataaaggc tgggcccttg
     2281 tccatcagca ccgctcgctg tagcgaccac accacgtctt cagcgctgat ggcgttgcca
     2341 gaggcaaact tgaggccggg tttgagctca aagccaaacg tc
//
LOCUS       DAOSLL010000670         2378 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_38584_length_2378_cov_3.346104, whole genome shotgun sequence.
ACCESSION   DAOSLL010000670 DAOSLL010000000
VERSION     DAOSLL010000670.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38584_length_2378_cov_3.346104"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(71..673)
                     /locus_tag="PLB25_20685"
     CDS             complement(71..673)
                     /locus_tag="PLB25_20685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HOE44023.1"
                     /translation="MLLEAELSQVVLVDYQARLMSAIFEAPLVLANAVRLAEIARILQ
                     IPVIGTEQNPSRLGENEPEIKLRCRQTLSKMQFSGVEEGLGEWLRAPVKPSGRNARSL
                     PKHMLERSATPDGRNSIVIAGCEAHVCLLQTALHLLEDEFDVWVVTDACSSRTERNRD
                     AAFDRLAGAGVELVTTEMVAFEWLRSAEHPQFKDVQAVIK"
     gene            complement(1126..1458)
                     /locus_tag="PLB25_20690"
     CDS             complement(1126..1458)
                     /locus_tag="PLB25_20690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011810617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HOE44024.1"
                     /translation="MNARNSVKYRLDPGHPPAVTDEQQTRLEAIAAMPDTAIDYSDIP
                     RQTEPVQWTRPGMMLPSENKQQITLRVDADVLSFFKSTGKRYQSRINAALREYVNTHR
                     SESDLSKP"
     gene            complement(1424..1633)
                     /locus_tag="PLB25_20695"
     CDS             complement(1424..1633)
                     /locus_tag="PLB25_20695"
                     /inference="COORDINATES: protein motif:HMM:NF016268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HOE44025.1"
                     /translation="MVFLDPDRIETFDGRDFYGEDRWKTVGRVSLALLSVVYTVRDKG
                     NETIRLISARKADAYERTQFGQVQT"
BASE COUNT          504 a          657 c          678 g          539 t
ORIGIN      
        1 tcgactgttt gtgtaggagt cgcgccctcg cgacgaaggc cgtccaattc cggcttgtcc
       61 ggcttagggt tcacttgatg acagcctgca catccttgaa ctgaggatgc tcggcgctgc
      121 gcagccactc aaacgccacc atttcggtcg tcaccagttc caccccggcg ccagcgagcc
      181 ggtcaaaagc ggcatcgcgg ttgcgctcgg tgcgcgaact gcaggcatcg gtcaccaccc
      241 agacatcgaa ctcgtcttcc agcaaatgca aggccgtttg cagcaggcac acgtgcgcct
      301 cgcaaccggc aatcacaatg ctgttgcgtc cgtccggtgt ggccgagcgc tcaagcatgt
      361 gtttgggcag gctgcgggca ttgcgtccag atggtttgac cggtgcgcgc agccattcac
      421 caaggccctc ttcgaccccg ctgaactgca tctttgacag ggtttgacgg cacctgagct
      481 tgatctcggg ctcgttttca cccagccggg atggattctg ctcggtgccg atcaccggta
      541 tctgcagtat ccgtgcgatt tctgccagtc tgaccgcatt ggccagcacc agcggcgctt
      601 cgaaaatggc agacatcagg cgcgcctggt aatcaaccag aacaacttgg gacagttcag
      661 cttctagcaa catgatggcc tttcaggcaa ggatggacaa cgagaggctc cattaaaccg
      721 caggacagcg gcgttttgct caagctgcct gctatggcac cttcatgcgt caagatcgcc
      781 agcgccgtgt ccccaacgtg gggccagcag tggcgcaaga ccaagcatca aatttggcac
      841 accagcgcgc tggccggtac agtggctgcg ctcggcgagc cacagctcaa gtcggctgga
      901 gtgtggcgca gttcatcagt caaattggcc tctagtgctt atggaataag cgctagcagc
      961 tattgaacgc agagcattgg cgaatgctgg ttggtacgca ggggctgggg ttgggctggg
     1021 acggctgcca ccaacgcatc gcctgaggca agcgactttt gaacctaagc aaaaaaggcc
     1081 gccattgatg gcagcagatg cagcatcaaa actgcgcaag atgcttcatg gcttgctcaa
     1141 atccgattct gatcgatgcg tatttacata ttcgcgcagc gccgcattga tgcggctctg
     1201 ataacgtttg ccggtgcttt tgaaaaaact cagcacatca gcgtcaacac gcaaagtgat
     1261 ttgctgcttg ttttcactgg gcagcatcat gccgggccgc gtccattgca caggctcggt
     1321 ctggcgggga atgtcactgt agtcaatcgc cgtatcgggc atggccgcaa tagcctccag
     1381 acgcgtttgc tgctcatctg tcacggcagg tggatgacct gggtcaagtc tgtacttgac
     1441 cgaattgcgt gcgttcataa gcatctgcct ttcttgcgga gattaaacga atggtttcat
     1501 tgcccttgtc ccgaaccgtg taaacaacag atagcagggc aaggctgacc cgtcccacgg
     1561 ttttccagcg atcttcacca taaaagtcac gaccatcaaa tgtctcaatt cgatcagggt
     1621 caagaaacac cagagctgcg tcctcaaagc agacacgatg cttgctttgg ttagccagtg
     1681 ctttcttgtg ttgatatctc agtcaaattc catacgctca atgtactacg cttgtagcta
     1741 catgaagtca cagccagatg ggcaatgacg gggcgaatga cttcccgccc ctcagtgtgg
     1801 cgatgagttt gccgcgcgat gccggacggc cattgggcat ggtcttgggc ttgggcttgg
     1861 gatagtggca caagccacgc accatcgcca gggcaatttg gtcaggtggg cggcgttgcc
     1921 ggagttggct ttggcgcaga cgaagatgac ttggtgctta tccagcgccg ccttgatttc
     1981 ttcactgcgg gcgctgaccg gctagggttc gggcaactcg atgttcaaag ctgcggacat
     2041 gtggccgcaa cgatcaaggc cgcgtcaggg agcggctgaa gtttggcttg ccctattggt
     2101 caaattggca tctagccctt gttgtataag cgcaagtagc tattgaaatt ggagccaatg
     2161 gcgaatcccg tgtgtctggt ggtgcgccgt ggctgtgact ggctggggac ttttgaacgc
     2221 agccagtcat accaaacgca agagggcaaa tcgatggctg aacagttgcc ttgccgtaac
     2281 aggtgccaat caggtgcgct ttttgttttg ctaatgcgac gacaacaaat ccctacacaa
     2341 ctcggaaccc tctttcgatt cgcgacggtc ttcggatt
//
LOCUS       DAOSLL010000671         2364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_38938_length_2364_cov_3.642702, whole genome shotgun sequence.
ACCESSION   DAOSLL010000671 DAOSLL010000000
VERSION     DAOSLL010000671.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38938_length_2364_cov_3.642702"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2285)
                     /locus_tag="PLB25_20700"
     CDS             complement(<1..2285)
                     /locus_tag="PLB25_20700"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HOE44026.1"
                     /translation="MEDPALRHVYYGFFGLIVGFYSFFYFYSGNWDYFFSGVWTHEPD
                     QLGDLFKSGLYINGEAWGIPKILSSPLILGVFVAAGVALGKALEYAYRKIVEKARLPL
                     TEPEIISRCLSFTAYVSINVFYIFGGRSNLMLLPGPVLRVIDILLVALTTLWFWQAIQ
                     RNPMQYRREGLASSLIGQLRKLNVDISKFLEGRKLEELKPDEVYVLAKTLPSFSREQR
                     MQAYRNILEEVLRTGKTDTSASLELLREVRVEVGISDDEHRQMLQEFGIDGADKLLDP
                     EKAATFENWIRLDNYRRVIEPILLGQLDLGHKLSGPLQDSAVARTVQEYREIYHISEV
                     EHANVIANLTGTGGVLIERAKRQLEMLADDAALVFGLRSQMLADRQWHSIANLLIAST
                     VRRINLLCAKLFAMLMTLGNTSEARLLAAEIAELMGEDIETPLSLPVTSGARVTWVES
                     LDTSLVNLLRGSQELECDIVLPLDGSGFHSVIKRGGDLAQNLKRTVATEDPLVGALAL
                     TAISYLDLGLARQVASEIERQDAGANRLMMEVIDGLTGKPSIKTVGIPDQAFNITLAN
                     FNSTQQVLSFMKDHVTVGRAGDNDIVVLGQVVSPYHLTISRQGNLVQVRKSDHFSGLF
                     VNGVAIEGDTAAIETGARVSFLPSSQPGPLLVIEWTEPNANYTIESHDTVTKLLWLSS
                     AKIFQEIDLGSLTEIASAVEIRRYAKDAWLFHAGEAAKDAFLLQAGTAEVLITRDGQT
                     SVVKTYQEGAVFGELGVITGHP"
BASE COUNT          563 a          663 c          618 g          520 t
ORIGIN      
        1 gggtgtccag taatgacacc cagttcacca aagacggccc cttcctgata agtctttacg
       61 acggaagtct gcccgtcgcg cgtaatcaag acttcggctg tgcccgcctg cagaaggaag
      121 gcatctttcg ctgcttcacc agcatgaaac aaccatgcat ccttggcgta gcgccgaatt
      181 tcaactgccg aggcaatctc cgtcaatgag ccaagatcaa tttcctggaa tattttggcg
      241 ctggacaacc aaagcagctt ggtcactgta tcgtgggact caatcgtgta gttggcattc
      301 ggttcagtcc actcgatgac aagcaatggc ccaggctggc tggaaggcaa aaaactgaca
      361 cgtgcgccgg tttcaatagc agcggtatcg ccctcaattg cgacaccgtt gacaaaaagg
      421 ccggaaaagt ggtcactttt acggacttgt acaaggttgc cctgacgcga gatcgtcagg
      481 tgataggggg aaacgacttg tcccagaacg acgatatcgt tgtccccagc ccgacccacc
      541 gtgacatgat ccttcatgaa tgacagcacc tgctgagtgc tgttgaagtt ggccaatgtg
      601 atgttgaatg cctggtccgg tatgccgact gtcttgatcg atggtttccc ggtcaagcca
      661 tcaatcactt ccatcatcag acggtttgcg cccgcatcct gacgttcaat ttcagaggca
      721 acctgccgcg ccagaccaag gtcaaggtaa gaaatggcgg ttagagcaag agcacccacc
      781 agcgggtctt ctgtggctac cgtgcgcttc agattctggg ccaggtcacc ccctcttttg
      841 atgacagagt gaaaacctga accatccaag ggcagcacaa tatcgcattc cagttcctgg
      901 ctgccgcgca gtagattcac aagtgaggta tccagcgact ccacccaggt aacccgcgcg
      961 ccgctcgtca caggcaggct caacggggtt tctatgtctt cacccatcaa ttcagcaatc
     1021 tctgcggcca gcaggcgtgc ctcgctggtg ttccccaggg tcatcaacat ggcaaacagc
     1081 tttgcacaaa gcaagttgat tcttcgcacc gtactggcaa tcaaaagatt ggcaatcgaa
     1141 tgccattgcc ggtccgccag catctggctg cgtagcccaa acaccagcgc tgcgtcgtcc
     1201 gcgagcattt caagttggcg cttggctcgc tcaatcagca ctccacctgt tccagtgaga
     1261 ttggcaatga cgttggcatg ctcgacttcc gagatatggt agatctcccg atattcctgg
     1321 acagtgcggg caacggcgct gtcttgcaat gggcctgaca gtttgtgacc aaggtcgagt
     1381 tgcccaagca aaatcggctc aatgacccgg cggtagttgt caagccggat ccagttttca
     1441 aacgttgcgg ccttctcagg gtcgagcaat ttgtctgcac cgtcgatccc gaattcctgc
     1501 agcatttgtc ggtgttcgtc gtcgctgatg ccaacctcaa cgcgcacctc gcgaagcaat
     1561 tcaaggctcg cgctggtgtc ggtcttgccc gtgcgcaaca cttcttcaag aatgttgcgg
     1621 taggcctgca tgcgctgctc gcgtgaaaaa ctcggcaggg tcttggccaa cacatagact
     1681 tcgtcaggct tgagttcttc aagtttgcgg ccctccagaa acttcgagat atcaacattc
     1741 agcttgcgca actgtcctat caggcttgat gccagtcctt cgcgccggta ctgcatcgga
     1801 ttgcgctgga tcgcctgcca gaaccaaagt gtggtcagcg ccacaagaag aatgtcaatc
     1861 accctgagca ccgggccggg caaaagcatc aggttggagc gaccaccaaa aatatagaag
     1921 acgttgatcg aaacataggc agtgaatgaa aggcagcgac tgataatttc gggttcagtc
     1981 aagggcaacc gggccttttc aacgattttg cggtaggcgt actccagcgc tttgcccaga
     2041 gccacgccag cggcaacaaa aacccccagt atcagtggcg aagacaggat ttttggtatg
     2101 ccccaggcct cgccattgat gtacagccct gatttgaaaa ggtcgccgag ttgatctggt
     2161 tcgtgcgtcc atacgcctga aaagaaatag tcccagttgc ccgagtagaa ataaaagaac
     2221 gagtagaagc cgacaatcag accaaagaaa ccgtaataga catggcgcag ggcgggatct
     2281 tccatgctct cccagtagga gcgctcaagg tcaatatcgg ggcagttcgc tgtgcatcca
     2341 acgcagatgc tgcgctcgcc ttcg
//
LOCUS       DAOSLL010000672         2364 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_38943_length_2364_cov_2.568211, whole genome shotgun sequence.
ACCESSION   DAOSLL010000672 DAOSLL010000000
VERSION     DAOSLL010000672.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38943_length_2364_cov_2.568211"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="PLB25_20705"
     CDS             complement(<1..679)
                     /locus_tag="PLB25_20705"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE44027.1"
                     /translation="MVVAVHPYPSPQLEDSFGRRFPYLRLSILDACNFRCSYCLPNGY
                     RAKDGRGKYLDVQEIQRLVSAFARLGMRKLRITGGEPSLRRDLPELISVAAEATGVEN
                     VGLTTNGATLHRHLNRWIDAGLTALKVSVDALCPERFERITGHAGLEMLLDAIDQALD
                     SPLRSVKINAVLLRDLNDDQLPKWLEYLRDRNVTVRFIELMQTGDNLEYFQRHHYRAQ
                     VLESHIVK"
     gene            1026..1544
                     /locus_tag="PLB25_20710"
     CDS             1026..1544
                     /locus_tag="PLB25_20710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013123656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOE44028.1"
                     /translation="MSEQPKPLWRPHSAGLLLGLGLLLTFLLTGHGLGGSGFTTALAA
                     KSAALVAPAATQANNYLGPMVEDGNNPLNSWISWQVLGVAIGALIGAVSARRFKLTVD
                     GPTRISQAGRLTLALLGGMVAGIGARISLGCTSGLGLSGAATLATAGFLFLIGFFVAG
                     AFFGLITRRLWK"
     gene            1553..2101
                     /locus_tag="PLB25_20715"
     CDS             1553..2101
                     /locus_tag="PLB25_20715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018911671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HOE44029.1"
                     /translation="MDLSIVVTGLVCGVVFGFALERAGFASACKLTAQLRFQDWAVFN
                     VMFTAIVVCAIGLYLFKILGLLSMEDVYVPTTYLWATLLGGVGVGAGMSIGGYCPGTS
                     VVGFMSGKVDGFVFFLGIIAGTWVFAGVYDWLEPMLEALPRPDAETLPELFGVPEWVI
                     LIALTAVAAIGGWITTKPKPAN"
     gene            2120..>2364
                     /locus_tag="PLB25_20720"
     CDS             2120..>2364
                     /locus_tag="PLB25_20720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44030.1"
                     /translation="MSKKQTNGSLGQSLSALLLAASAISAAPVVCAQTEQAPTSAEET
                     TTETTADTNTSESGESDSASTPANPCAPKPKKKPKNP"
BASE COUNT          538 a          636 c          653 g          537 t
ORIGIN      
        1 ccttgacgat gtgtgactcg agcacttgcg cacgatagtg atggcgctgg aaatactcga
       61 ggttgtcgcc agtctgcata agttcgatga atcgaaccgt gacattgcgg tctcgaaggt
      121 actcgagcca cttcggcagt tgatcatcgt tgagatcacg taacaaaacg gcgttgatct
      181 tgaccgatcg caaagggcta tcaagcgctt gatcgatggc gtcaaggagc atctcgaggc
      241 ctgcatgccc ggtgatccgt tcaaatcgct ctgggcacaa agcatccacg ctgactttaa
      301 gggcggtgag tcccgcatca atccagcggt tcaagtgtct gtggagggtc gcgccgtttg
      361 tcgtgagtcc gacgttttcg acgccagtag cttcggctgc gacggaaatc agttccggca
      421 gatcacgacg cagacttggc tcaccaccag ttatccgcag cttacgcatg cccaagcgtg
      481 caaaagcact cacaagacgt tgaatttcct ggacatcaag atattttcca cggccatcct
      541 ttgccctgta gccgtttggc aggcagtagc tgcatcgaaa attgcatgcg tccaagattg
      601 acagtctcaa gtaaggaaac cgacgaccaa aactgtcttc aagctggggg ctgggatagg
      661 gatgcacggc gaccaccaca agtttcagcg tacgttatcg caaaacataa cgcgggcacc
      721 atggtcgaag cgtgatggct agcgccagct tggcataagc cttcacgcac atggaaagtt
      781 gggaatacat gggaagaagc acgtcaaggt tcgcaagtat ctgaattaga cggtgttgat
      841 gtacgaaacc ggcatggcgc aaaacaatca atcaacgcat aatgtatatt atgtccgcat
      901 tcttgcaccc aagttgcacg gtgagcaaca ggttgctgcg ccgcacgatt gacagtaatc
      961 aaacggtaca taaagtgggc tccataccca tgggggtatc ttcagccaac ctttggagag
     1021 gatgagtgag cgaacaacca aaacccttgt ggcgacccca ttccgcagga ctgcttctcg
     1081 gtctcggtct gctgctcacc ttcctgctca ccggtcacgg cttgggcgga agcggcttca
     1141 ccactgcctt ggcagctaaa agtgcagcac tcgtggcacc agccgcgacc caagccaaca
     1201 actatcttgg gcccatggtt gaagacggca acaatcccct gaactcttgg atctcctggc
     1261 aagttcttgg agtcgcgatc ggtgcgttga tcggtgcggt cagtgcgagg cgattcaaac
     1321 tgactgtcga tgggccaacg cgcatctcgc aagcaggacg cctaaccctt gccctactcg
     1381 gtggaatggt cgcgggaata ggagcccgta tctctctcgg ctgtacgagc ggtcttgggc
     1441 tctctggagc ggcgacactc gccaccgcag gattcctctt tctcattggt ttttttgtag
     1501 ccggtgcgtt cttcggatta atcacgcgga ggctgtggaa atgatgttcc caatggattt
     1561 gagcattgtt gtcactggtc tcgtctgcgg tgtggtcttt ggatttgcgc tggaacgcgc
     1621 cggcttcgca agtgcctgca agctaacggc ccagctacgc tttcaggact gggctgtatt
     1681 caatgtgatg ttcacagcga ttgtcgtgtg cgctatcggg ctgtatttat tcaaaattct
     1741 tggcttgcta tcgatggagg acgtctacgt ccctactacg tacctttggg caacgctttt
     1801 agggggcgtc ggcgttggcg cggggatgag catcggtggt tactgtccgg ggacctccgt
     1861 tgtcggcttc atgtctggga aagttgatgg cttcgtcttt ttcctgggaa ttatcgctgg
     1921 tacatgggtg ttcgctggcg tctacgactg gctggaacct atgttggaag ccctgccacg
     1981 cccagacgcg gaaaccctcc cggaactatt tggtgtaccc gagtgggtca ttttgattgc
     2041 gctaaccgca gttgcggcca ttggtggttg gatcacaacc aaaccaaagc ctgccaacta
     2101 aacacaagag gattcagtca tgtcaaagaa gcaaacgaac ggaagccttg ggcagtcact
     2161 ttccgcactg cttttggccg caagtgcgat ctccgccgca ccggtggtgt gtgcccagac
     2221 cgaacaggca ccaacatccg ccgaggaaac cactaccgaa accacggcag acacaaatac
     2281 gtcggaatct ggcgaatccg acagcgccag caccccggca aatccatgcg cacccaaacc
     2341 gaagaaaaag ccgaaaaacc cttg
//
LOCUS       DAOSLL010000673         2363 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_38959_length_2363_cov_3.468371, whole genome shotgun sequence.
ACCESSION   DAOSLL010000673 DAOSLL010000000
VERSION     DAOSLL010000673.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38959_length_2363_cov_3.468371"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="PLB25_20725"
     CDS             complement(<1..716)
                     /locus_tag="PLB25_20725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019430251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HOE44031.1"
                     /translation="MSKKVFIKTYGCQMNEYDSDKISDVLGAAQGYELTQDVNLADLI
                     LFNTCSVREKAQEKVFSDLGRVRHLKKRGALIGVGGCVASQEGAAIIERAPYVDVVFG
                     PQTLHRLPQLLAERERLNRPQVDISFPEIEKFDHLPPARIEGASAFVSIMEGCSKYCS
                     YCVVPYTRGEEVHRPFEDLLVEVAGLADQGVKEITLLGQNVNAWRARMTSHPGAAGSS
                     SEMADFATLLEYVSDIPGIER"
     gene            complement(796..2097)
                     /locus_tag="PLB25_20730"
     CDS             complement(796..2097)
                     /locus_tag="PLB25_20730"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HOE44032.1"
                     /translation="MTFSSLGLSPALQAGVLALGHSHLTPVQRAAIAPALQGQDILAT
                     AQTGSGKTLAFALPLLQRLRAPDSRHRQVQALVLVPTRELALQVGEVIRTLARPLPHG
                     IKVSSLYGGVSVNPQMMGLRGGTDVVVATPGRLLDLARHNALLLDQTALLVLDEADRM
                     LDLGFADELAQVLALLPRQRQNLFFSATFSPPVQALAQALLHDAVRVDIAQPGQQPAA
                     IAQRAIAVDASRRTQLLRHLIALHGWPRVLVFVATKYAAEIVADKLRKAGLTAEPFHG
                     ELSQGKRSQVLTDFKAGRLAVMVATDMAARGLDIAELPVVVNFDLPRSTQDYTHRIGR
                     TGRAGVPGQAISFVSAATQAHFRLIEKRQQVRLSLETVPGFEPKELAAPASPDPNAPA
                     NGGIKGRRPSKKDRLRAQGGLPLPAADAGVDTDGGASGAAR"
BASE COUNT          430 a          727 c          748 g          458 t
ORIGIN      
        1 cgctcaatgc cgggaatgtc cgacacgtat tccagcaagg tggcaaagtc agccatttcg
       61 ctgctgctgc ccgcagcgcc ggggtggctg gtcattctgg cgcgccaggc gttgacgttc
      121 tggcccagca gggtgatttc cttcacgccc tggtcggcca ggcccgcgac ctcgaccagc
      181 aggtcttcaa acgggcggtg cacctcctcg cccctggtgt agggcaccac gcagtagctg
      241 cagtatttgg agcagccttc catgatcgag acaaaagcgc tggcaccctc gatgcgggct
      301 ggcggcaggt ggtcgaactt ttctatttcg ggaaagctga tgtccacctg cggacggttc
      361 agccgttcgc gctcggccag cagttgcggc aggcggtgca gggtttgcgg gccaaacacc
      421 acatcgacgt agggcgcgcg ctcgatgatg gccgcgccct cctggctggc aacgcagccg
      481 cccaccccaa tcagagcgcc gcgctttttg aggtgtcgca cccgtccgag gtcagagaac
      541 actttttctt gcgccttttc gcgcaccgaa caggtgttga acaaaatcag gtcggccagg
      601 ttcacgtcct gtgtcagttc gtagccctgg gcggcaccca gcacatcgct gatcttgtcc
      661 gagtcgtact cgttcatctg gcagccgtag gttttgataa agactttttt gctcatctcg
      721 aattcattca ttcactataa atttagaagc tgtcagcgca atgcctgtaa gggctaaagg
      781 ccaatttgat atgaatcagc gggccgcccc gctggccccg ccatcggtat cgacaccggc
      841 atcggctgca ggcagcggca atccgccctg cgcccgcagc cggtctttct tgctcggccg
      901 acggcccttg atgccgccgt tggccggcgc attggggtct ggcgaggcgg gcgctgccag
      961 ttccttcggc tcaaagccgg ggacggtttc cagactcagc ctgacctgct ggcgcttctc
     1021 gatcagcctg aaatgggctt gcgttgcagc actgacaaag ctgatcgcct ggcctggcac
     1081 gccggcgcgc ccggtgcggc caatgcggtg ggtgtagtcc tgcgttgagc gcggcaggtc
     1141 aaaattgacc accaccggca gctcggcaat gtcgagcccg cgcgcagcca tgtcggtggc
     1201 caccatcacg gccaaccgcc cggccttgaa atcggtcagc acctggctgc gcttgccttg
     1261 gcttagctcg ccgtgaaacg gctcggctgt caggccggcc ttgcgcagct tgtcggcaac
     1321 aatttctgcg gcgtatttgg tggccacaaa caccagcacc cgtggccagc catgcagggc
     1381 gatcaggtgg cgcagcagtt gggtacgccg actggcatcg acggcgatgg cgcgctgggc
     1441 gatggctgcc ggttgctggc ccggctgcgc aatgtccacc cgcacggcat cgtgcaacag
     1501 ggcctgtgcc agggcctgca cgggcgggct gaaggtggct gagaaaaaca ggttttgccg
     1561 ttgccggggc aatagcgcca gcacttgcgc cagttcgtcg gcaaaaccca ggtccagcat
     1621 gcgatcagct tcgtcgagca ccagcagcgc ggtctggtcg agcaacagtg cgttgtgccg
     1681 ggccaggtca agcagtcgcc cgggtgtggc caccaccacg tcggtgccac cgcgcaagcc
     1741 catcatttgc ggattgaccg aaacaccgcc atacaggcta gacaccttga tgccatgcgg
     1801 cagtggccgg gccagcgtgc ggatgacctc gcccacctgc agcgccagtt cgcgtgttgg
     1861 caccagcacc agcgcctgca cttgtcggtg gcggctgtca ggggctcgca ggcgctgtag
     1921 cagtggcaac gcaaacgcca gcgttttgcc ggagccggtt tgtgccgtgg ccagaatgtc
     1981 ctgaccttgc agcgctggtg caatggccgc acgctgcacc ggtgtcaggt ggctgtggcc
     2041 cagcgcgagg acgcccgctt gcagcgcagg cgacaagccc agtgatgaaa aagtcatgtt
     2101 tgcctggtgg gtaaggacag ggtcgaaaac aagttacgca tttggccgct ggatttggca
     2161 tgcaatgcca ggcgcaaatc actcatgtgg cgagccacat gagtgatttg ccacgacgca
     2221 gtgcgtccaa acccgaagag ccaaatgggg aagtgattga tgaccatgtc ctaagttaaa
     2281 tgagcggaac accgggcagt ttacgtgctg cgctaaggat gtggtcagaa acaacttccc
     2341 ctatttgcac cggcggattt ggg
//
LOCUS       DAOSLL010000674         2355 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_39132_length_2355_cov_4.318696, whole genome shotgun sequence.
ACCESSION   DAOSLL010000674 DAOSLL010000000
VERSION     DAOSLL010000674.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2355
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39132_length_2355_cov_4.318696"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PLB25_20735"
     CDS             complement(<1..443)
                     /locus_tag="PLB25_20735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018905445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HOE44033.1"
                     /translation="MDGTIIDSMPSHAQSWITFIENHGITVDVPDLMRRTTGLTGAEC
                     MRELFQRDLSDEEAWALITEKEVIYRDLFAPRFAEVAGFKAFARQALARGLKVGVGTA
                     GDRHNIAFAMHHLALPIPPDAIVGGDEGLPGKPHPAIFLEVAKRMA"
     gene            complement(521..1625)
                     /gene="prfB"
                     /locus_tag="PLB25_20740"
     CDS             complement(join(521..1543,1545..1625))
                     /gene="prfB"
                     /locus_tag="PLB25_20740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013594222.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HOE44034.1"
                     /translation="MEAERINAIGTQLVGLSARSVELRGYLDFDAKFERLRTVNASLE
                     DPAVWSDPKRAQELGKEKKELDGVVVTLTDLQRDLSDNTELFEMSKDDGDEDGLLHIE
                     TDAARLNDIVQGLEFRRMFNNPADPLNCFLDIQAGAGGTEACDWASMLLRQYLRYAER
                     KGFKTELEDETPGDTAGIKGATIKIEGEYAFGLLRTETGVHRLVRKSPFDSSGGRHTS
                     FASVFVYPEIDDSIEIDINPADVRVDTFRASGAGGQHINKTDSAVRLTHLPTGIVVQC
                     QDGRSQHSNRDVAWKRLRSRLYDFELRKQQEAQQKLEDTKTDVGWGHQIRSYVLDNSR
                     IKDLRTNYETSATQKVLDGDLDAFIEASLKQGV"
     gene            2163..>2355
                     /locus_tag="PLB25_20745"
     CDS             2163..>2355
                     /locus_tag="PLB25_20745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44035.1"
                     /translation="MGDKLTNMLSIFADSLGQQASRFEPVVGHGCSPKANIIFPACRG
                     KCLVSNKSSLCISCEEFKHS"
BASE COUNT          471 a          698 c          649 g          537 t
ORIGIN      
        1 gccattcgct tggccacttc caaaaatata gcaggatgag gctttcctgg cagcccttcg
       61 tcgcccccga caatggcatc aggcggtatt ggcagcgcca ggtggtgcat cgcaaacgca
      121 atgttgtggc ggtcaccggc ggtgccaaca cccactttca ggccgcgcgc cagggcttgc
      181 ctggcaaacg ccttgaagcc ggccacctcg gcgaacctgg gcgcaaacaa gtcacggtaa
      241 atcacttcct tctcggtgat cagtgcccag gcttcttcat ctgacaagtc gcgctgaaac
      301 agttcacgca tgcattcagc gccggtgagc ccggtggtac ggcgcatcag gtcaggcacg
      361 tcaacggtaa tgccgtggtt ttcgatgaag gtgatccagc tttgcgcatg acttggcatc
      421 gagtcgatga tcgtgccatc catgtcaaaa atcaggccag aaacggctcc agcccatatg
      481 ggataagcgt cagcagctcc tgaagatgta gcaatcagca ttacacgccc tgcttcagcg
      541 acgcttcaat gaaagcatcc agatcaccat cgagcacctt ttgcgtggcc gatgtctcgt
      601 aattggtgcg caggtccttg atgcggctgt tgtccagcac ataactgcga atctgatggc
      661 cccagccgac atccgtcttg gtgtcttcga gcttttgctg ggcttcttgt tgcttgcgca
      721 actcaaagtc gtacaggcgg ctacgcaggc gcttccaggc gacatcgcgg ttgctgtgct
      781 ggctgcgccc gtcctggcac tgcaccacga tgccggtggg aaggtgcgtc aggcgcaccg
      841 cagagtcggt cttgttgatg tgctgaccac cggcaccact ggcgcggaag gtatcgaccc
      901 gcacatcggc cgggttgatg tcgatttcaa tcgagtcgtc aatttccgga tagacaaaca
      961 cgctggcaaa actggtgtga cgcccgcccg atgaatcaaa cggactttta cgcaccagcc
     1021 ggtgcacgcc ggtttcggtg cgcagcaggc caaacgcgta ttcaccctca atcttgatgg
     1081 tggcaccctt gatgccagcc gtgtcaccgg gggtttcatc ttcgagctcg gtcttgaacc
     1141 ccttgcgctc ggcgtagcgc aggtactggc gcaacagcat gctggcccag tcgcaggcct
     1201 cggtgccacc ggctccggcc tggatgtcca aaaagcagtt caatggatca gccgggttat
     1261 tgaacatgcg gcgaaattcc aggccttgca cgatgtcatt caacctggcc gcatcggtct
     1321 cgatgtgcag caagccgtct tcatcgccat cgtccttgct catctcgaac agttcggtgt
     1381 tgtcagacag gtcacgctgc aggtcggtca gggtgaccac cacgccgtcg agctccttct
     1441 tttccttgcc caattcctgg gcgcgcttgg ggtcgctcca gacggccggg tcttccagcg
     1501 aggcgttgac ggttctcagt cgctcaaact tggcatcgaa gtcaaagata cccccgtaat
     1561 tcgacactgc gagcgctcaa accgacaagt tgagtgccga tggcgttgat acgttctgct
     1621 tccatgatga gggtcctggt attttgatga attctactga gatcagcatt ttctcacgcc
     1681 gtttggctag tcgatgcttt tgccggaagt tgcgccagca gggcttcaaa tgcggcaacg
     1741 caacgggcat caaccttgag catggcaatc gccctgggcc aggtattacc cacttcaatc
     1801 aggtgctgca gacttgatcc ggtgaagttg caggattccc ggcacatcaa tcagcagcag
     1861 cgcctgtacg gtacccagca gccattgatg cattgctttc atgatcctgg atccctcagt
     1921 tgaccgcttg ttttcatgta caagtcctta tgagcatttt ctttttcagc ttcgatggag
     1981 ttcaccattt gggtgacagt gctacgcacc cgattgaact gctggcaacg cgcctggggc
     2041 gcaagctcct tctagcgggc agtcgcggcg cgtcgtcgtc gcgcctaccc agccgcgccg
     2101 ccagcggccc actcgggcgc gtccacccac tcgggcgcgt ccgacagagg aatccaggcg
     2161 ctatgggcga caaacttacc aacatgttgt cgatctttgc cgacagtctc gggcagcagg
     2221 catcccgttt tgaacctgtt gtgggccatg gctgttcgcc aaaggcgaac atcattttcc
     2281 cagcttgtcg gggaaaatgc ctggtctcta acaagtcaag tctttgcata tcatgcgaag
     2341 aattcaaaca ttcaa
//
LOCUS       DAOSLL010000675         2345 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_39348_length_2345_cov_4.118341, whole genome shotgun sequence.
ACCESSION   DAOSLL010000675 DAOSLL010000000
VERSION     DAOSLL010000675.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39348_length_2345_cov_4.118341"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(45..623)
                     /locus_tag="PLB25_20750"
     CDS             complement(45..623)
                     /locus_tag="PLB25_20750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44036.1"
                     /translation="MKLRSIALAAIAAFSFGAQADLYNQNVTNNVIMGTGISNGGFTT
                     ETGAGVEIGLRARERYDLATNSPTNVTGSNNNGTYNQNAGTPGSPTRARWNFDWSINT
                     GTANVGAYTYLLGMDFDAGVGTNFQMFDLINVTLADHSFGDNSTAQSAGVEAADAGTY
                     ASYLASKNLVQNSWNYDFFDSPSFTFSAAANE"
     gene            complement(1095..2042)
                     /locus_tag="PLB25_20755"
     CDS             complement(1095..2042)
                     /locus_tag="PLB25_20755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44037.1"
                     /translation="MSKEQSKPVRTIGISQSSVTSQPHTSRKNRRTSDDASQLLHLTA
                     RLHTTRGSTVDWRQLLQDCRDGFQCPGALEQLHDDAAIGPDELAALAGRISHCASFGD
                     HCGNDHLIDEQALNFKRQRCSALALHLHTAALAARKSLQAGLFDHLPPTWVLDRSGRQ
                     CEANTSARELTRVGERFGLREGLLVLTDPSASRMLRKALADARDDSRFLWQERHNLET
                     SLLLRPLPDGLHVAVTLVQDAPGVTELAPLLASHFGLTPRQSDLAAHLLADHTLAGAA
                     RAMGISLHTANEHLAALSEKVGAPDRKSLLVVLRHSVKQ"
BASE COUNT          495 a          631 c          661 g          558 t
ORIGIN      
        1 ggcgttgtgc gtcttcatca gcgttgtcag gcgcaagata aacgctattc gttagctgct
       61 gctgagaaag taaatgaagg actgtcgaag aaatcgtaat tccaagaatt ttgaaccaga
      121 tttttcgaag ctaaatagct tgcgtaagtg ccagcatcag ccgcttcaac tccggcgctt
      181 tgggcggtgc tgttgtcgcc aaaagagtga tcggccaacg tcacgttaat taggtcgaac
      241 atctgaaaat tggtaccaac acctgcatcg aagtccatac cgagcaggta ggtgtaagca
      301 cctacattgg ctgtaccggt gttaatcgac cagtcaaaat tccatcgtgc tctagttgga
      361 cttccaggcg tgccagcatt ctggttataa gtaccattgt tattgctgcc agtgacattg
      421 gtaggagaat ttgtcgccag gtcgtaacgc tcacgggcac gaagtccaat ttcgacacct
      481 gcgcccgtct ccgtggtgaa cccgccattg ctaataccgg tgcccatgat cacgttgttt
      541 gtgacatttt ggttgtaaag gtctgcctga gcgccaaacg agaaagcagc gatggctgct
      601 agtgcaattg aacgaagttt catagttgtc tttcagttga aaattaaacc gactttcccc
      661 tcagaagagc cactctgcaa aagtcggttt cttgacgcct tctccagctt ttctcttccg
      721 aaaacaaaaa acacaatgtg tgccaagtgt ctatatactt ttaaaccatt gatttatata
      781 aagttttttt acatgtcaga attcttgaca tttacttgtc aagttgcctt taccccacac
      841 tgtgacggga tacgttggtc tcagtttgag aaatggactg caagaatggg gcaccttttc
      901 tgcgtagaca cctttggaac ccagttttga tgtgaattgc gcggcctatt ccccacagag
      961 tgttgggttc cggcgatgac gctcttggct tgcaactgta aaaaaacccg acttatctgg
     1021 gcgcaagccc tgcaattcac gaacgccctg ccaagctgcc aagaccaaga ggtctcggcg
     1081 gcgtttggtc cgccctattg tttaacgctg tgccgcagca caaccagcag cgacttgcgg
     1141 tcgggcgcgc cgaccttctc cgacagcgcc gccaggtgtt cgttggcggt atggagtgaa
     1201 atgcccatgg cccgggccgc gccagccagc gtgtggtctg ccagaaggtg agcagcaagg
     1261 tcgctttggc gaggtgtcag gccaaaatga cttgccagca gcggtgccag ttcggtcaca
     1321 ccgggcgcat cttgcaccag cgtcaccgca acgtgcaggc cgtccggcaa tggccgcagc
     1381 agcaaactgg tttcaaggtt gtggcgctct tgccacaaaa agcggctgtc atcgcgggca
     1441 tctgccaagg ctttgcgcag catgcggctg gcacttggat cggtcagcac caacaggcct
     1501 tccctcaacc caaagcgctc gccaacacgc gtcagttccc gcgcactggt attggcctcg
     1561 cattgtcggc cgctgcgatc aagcacccag gtgggtggca agtggtcaaa caagcctgcc
     1621 tgcaacgact tgcgcgctgc cagcgcggcg gtgtgcaggt gcagcgcaag ggccgagcag
     1681 cgctgacgct tgaagttcag cgcctgttcg tcaatcagat gatcgttgcc acagtggtcg
     1741 ccaaaactgg cgcagtggct gatgcgaccg gccagcgcgg ccagttcatc gggtccgatg
     1801 gcggcgtcat cgtgtaactg ttcaagcgcc ccagggcact gaaaaccgtc gcggcagtct
     1861 tgcagcagtt gtcgccaatc aacggtactg ccccgcgtgg tatgcaggcg cgccgttaaa
     1921 tgcaagagct ggctggcatc gtcgctggtg cgtcgatttt ttcgtgaggt gtgaggctgg
     1981 ctggtcaccg atgactggct gatgccaatt gtgcgaaccg gcttgctttg ctctttggac
     2041 atggatctgc ttggggtttt ccacgacgtc ttgcagaatc tgcgaccgtc ttctgtggac
     2101 ggttcgatgt atttgtaacc gctatcgcag gatgcccaca ggtcaaatgc ctgcgggtcg
     2161 catccggccc aagccgggcc tggttgccag caagtcgctg taaccaggcg tttgaaaagc
     2221 gattaacgcc ctttttggat ggcggcaacg gctgcttcac cgtttttcag ggcggtgtca
     2281 agcgcggttt ccatggcagc ggcggcggct ttgatcaggg ggcgctgatc gccggcgcgg
     2341 tgatc
//
LOCUS       DAOSLL010000676         2327 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_39741_length_2327_cov_4.801496, whole genome shotgun sequence.
ACCESSION   DAOSLL010000676 DAOSLL010000000
VERSION     DAOSLL010000676.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39741_length_2327_cov_4.801496"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..939
                     /locus_tag="PLB25_20760"
     CDS             <1..939
                     /locus_tag="PLB25_20760"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE44038.1"
                     /translation="LFENLSVEASTLVTHAETAMYRAKETGCNLIRFFDSSTQAVLQT
                     RFMLNEWMRQGLPDQFTLHYQVQTDHLGLPFGAEALLRWQHPEQGAISPAMFIPLAEE
                     TGFIIEIGAWVLRTACEQLVLWSDDPAMQHLTLSVNVSARQFNQKDFAQQVLATLTHT
                     GANPLLLELELTEGMMVRDMEAVIEKMEMLKRHGVRFALDDFGTGYSSLSYLGRLPLD
                     KLKIDQSFVRGLQDDPSAAAIVRAVITLGQSLDIAVIAEGVETTEQCQFLQTNGCQEF
                     QGYFFGRPIDLTAFEGLVRDPSKKLELVKLGVAVLV"
     gene            1439..>2327
                     /locus_tag="PLB25_20765"
     CDS             1439..>2327
                     /locus_tag="PLB25_20765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011463223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE44039.1"
                     /translation="MFANMKIGLRLTLGFAIVLVLMAVLAAVGINGMSTVQNHLDDIV
                     RDNMHKIKLNTDISESVHIVTRITRTIILLTDAAAMDAEKKKLDGVRQKYNEAWETLD
                     KLPASEKGKAARAKIKNAAAEARPLTDKVLELAMSNKNDEALKLLMSQAAPAAQKWQD
                     AIDENVTLQEENTKNDVASSQKSYDSARTLMLTLASVAIALGSLTAWVLTRSVTLPVN
                     EALNAANRLAEGDLTVRIDNPSKDETGQMLQAMSSMITKLSQVVTDVNGGAQALASAS
                     EEVSATAQSLSQAASEQAAG"
BASE COUNT          559 a          601 c          675 g          492 t
ORIGIN      
        1 ttgtttgaaa acctgagtgt ggaagcgtcg accttggtca cccacgctga aactgccatg
       61 taccgggcca aggaaaccgg atgcaacttg attcgattct ttgactccag cacgcaggcg
      121 gtgctgcaga cccgtttcat gctcaatgag tggatgcgtc agggcctgcc agaccagttc
      181 acgttgcatt accaggtaca aaccgaccat ctgggtttgc cctttggcgc cgaagcgttg
      241 ctgcgctggc aacacccgga gcaaggcgcc atttcacccg ccatgtttat cccgctggct
      301 gaagaaacgg gcttcatcat tgagatcggt gcctgggttt tgcgaacagc ttgcgagcaa
      361 ttggtgttgt ggtcagacga cccggccatg caacacctca ccctgtcagt caacgtcagc
      421 gccaggcagt tcaatcagaa ggactttgcc cagcaggtgc tggccacgct gacgcatacc
      481 ggagccaacc ctttgttgct cgaactggag ctaaccgagg gcatgatggt tcgcgatatg
      541 gaagctgtga ttgaaaaaat ggagatgctc aagcgccatg gggtgcgctt cgcattggac
      601 gactttggga ccgggtattc gtccttgagt tatctggggc gccttccgct ggacaagttg
      661 aagattgatc agtcgtttgt gcgcggcctg caagatgacc ccagtgctgc agccatcgtg
      721 cgggcggtga tcaccctggg tcaaagtctt gacatcgccg tgattgccga gggcgtcgag
      781 acgactgagc agtgccagtt tttgcaaacc aacggttgcc aggagtttca aggctatttt
      841 tttggcagac cgatagacct gacggcgttc gaaggcctgg tgcgcgaccc gtccaaaaaa
      901 cttgagcttg tgaagctggg cgtagcggtg ttggtttgaa aaccagaaga ggatttcatg
      961 gcttatcaaa tcacagtaga aaaatttggg gtgtattcca agtactccgg gcttgtcacg
     1021 ggtgcagagt ttctgcaatc gatgttgcac aaccggagcg accctgatta ggcagcccaa
     1081 gtcaccactg ttcctggccc ctggtttgtc aacacccggt tattagcctt tgttaaaggg
     1141 aagtcatggc aaaaagagtg tcagcctgga tctgaaacac tattgtttca gtagctgctt
     1201 acgcaagtgg acaaagggct ggagggcaaa aaggcttgaa agtgcaagct aacaagcggt
     1261 ttggaatggt caaagtgaaa caacaccgga tgttctgtag gagcggcgcc ctcgccgcga
     1321 atgcgaaagc tggagttcgc ggcgagggca ccgctccgac aaagtttcat tcttcggggt
     1381 gggcgagcgc ccaagccggt tatatgggtg tttaaacagt ttttaaaggg gtattgagat
     1441 gtttgcaaac atgaaaattg gcctgcgctt gacgctgggt tttgccattg tgctggtgct
     1501 catggcggtg ctggcggcag tgggcatcaa cgggatgtca acggtgcaga atcaccttga
     1561 cgatatcgtc cgggacaaca tgcacaagat caagctcaac acagatatct cggagtctgt
     1621 tcatatcgtg acacgcatta cccgcaccat cattttgctt accgatgctg ctgcgatgga
     1681 tgccgagaag aaaaaacttg acggcgtacg ccaaaagtac aacgaagcct gggagacact
     1741 ggacaaattg cccgccagcg agaaaggcaa ggcagcccgc gccaagatca aaaatgccgc
     1801 tgccgaagcg cgcccattga ccgacaaggt cctggagctt gccatgagca acaaaaacga
     1861 cgaagccttg aagctgttga tgagtcaggc cgcgcccgct gcccagaaat ggcaggacgc
     1921 aattgatgaa aacgtgactt tgcaggaaga aaacacaaag aacgatgtcg cttcctctca
     1981 aaaaagctac gactcggctc gcaccttgat gctgacgctg gcctcagtgg ccattgcctt
     2041 gggttcgttg accgcctggg tgttgacccg ttcggtcacc ctcccggtca acgaagcctt
     2101 gaacgcggcg aaccgcctgg ccgaaggtga cctgacggtg cgtattgaca acccgtccaa
     2161 agacgaaacc ggccagatgc tgcaagccat gagcagcatg atcaccaagc tcagccaggt
     2221 ggtgaccgat gtgaatggtg gtgcccaggc actggccagt gcatcggaag aagtcagcgc
     2281 cacggcacaa agcctgtcgc aagccgccag tgaacaagcc gccggtg
//
LOCUS       DAOSLL010000677         2326 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_39767_length_2326_cov_4.040070, whole genome shotgun sequence.
ACCESSION   DAOSLL010000677 DAOSLL010000000
VERSION     DAOSLL010000677.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39767_length_2326_cov_4.040070"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="PLB25_20770"
     CDS             complement(<1..465)
                     /locus_tag="PLB25_20770"
                     /inference="COORDINATES: protein motif:HMM:NF020445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DndE family protein"
                     /protein_id="HOE44040.1"
                     /translation="MADRLYTSSEADEVLSALRFETKLEKATLARIAFTLSLVKDGAG
                     VAASLSFSGGELKRPTFIAEDEVFLRTLIAHTYSKRDFSEDEFYSNRSIVKNHIDSGA
                     ALLAKLFQETGRSGEALIRRLVDEVEFAGRREQLGSGLDIFIGRTLLQRTELI"
     gene            complement(476..>2326)
                     /gene="dndD"
                     /locus_tag="PLB25_20775"
     CDS             complement(476..>2326)
                     /gene="dndD"
                     /locus_tag="PLB25_20775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA sulfur modification protein DndD"
                     /protein_id="HOE44041.1"
                     /translation="VKPLLSPRQSRGITCFIYRNINRREKAKGNANVAFELVIEMDDA
                     SELVVKRAWTAGAVNEPKARDLTERLVVVRDGKRVSVQNQEIWQNFIRAAIPPGITQF
                     FFFDGEKIQEIAADDHSEMRLKSSLEAALGIGHINRLAADIIYIKQEERKGFVEISDE
                     DLEFKQSELKREKSKLTRKHHERDSLRAELDGFKGQLDEAKKRFEATFQIAPESRETM
                     REQEKKRVQAANRLAQVESEIRSLAEKALPFSIAGKLFAGIRGQIELERESATGEAIK
                     ENAAALARRIVRVVEDPEPIYTEKLSPERMAELERRIYRLLKEGDQSAAVAKVLDLSD
                     RDAARVLNRMESLEGSDVFLLKALLDEARSLAVQVRQLEGVGQSGGLSGTERDLFDEL
                     QGVMESCSTQIGRKTEQLRLVEEDILALTKRIGDIEVEIEKLFEKHNVSKDKADFIEE
                     CDSIASVLNKFIVRLRKNKVQLLQEKTFEMYRLLSSRSGLIKDITIDDQSYEIHIIDR
                     NGHEIRKSSMAAGEKEVFAISLLWGLAQTSELKLPIIIDTPLSRLDSTHRDNIVNNYF
                     PNAGEQVIILSTDTEIDTNYYRALKPRLAGAASLEFDQRQELTTFRQGYF"
BASE COUNT          446 a          707 c          605 g          568 t
ORIGIN      
        1 gattagctcg gtccgctgca ataaggtgcg gccaataaaa atgtccagcc cgctacccag
       61 ttgctcgcgc cgaccggcaa actccacctc atcgaccagc cgccgaatca gcgcctcgcc
      121 actgcggcca gtttcctgaa acagctttgc cagcaaagct gcgccactgt caatgtgatt
      181 tttgacaatg gaccggttgg aataaaactc gtcttcgctg aaatcccgct tgctgtaggt
      241 gtgcgcaatc agggtgcgga gaaacacctc gtcttcggca ataaaggttg gccgcttcag
      301 ttcacccccg ctgaaactta ggcttgccgc cacaccggca ccgtctttga ccagcgacag
      361 cgtaaaagcg atgcgcgcca gcgttgcttt ttccagcttg gtctcaaacc gcagggcgct
      421 caacacctca tcggcttcgc ttgatgtgta caggcggtca gccatgattt ttctctcaaa
      481 agtaaccttg cctgaacgtc gtcagctcct ggcgctggtc aaattccaga cttgcagcac
      541 cagccaggcg cggtttcagc gcgcggtaat agttggtgtc aatctcggtg tcggtcgaga
      601 ggatgatcac ctgctcaccc gcgttgggaa aatagttgtt cacgatgttg tcgcggtgcg
      661 tgctgtccag gcgcgacaag ggcgtgtcga tgatgatcgg cagcttgagt tcgctggttt
      721 gcgccagccc ccacagcagc gaaatcgcaa atacctcttt ttcaccagcg gccatagacg
      781 attttcggat ttcgtgcccg ttgcggtcaa tgatatgaat ctcgtagctc tggtcgtcga
      841 tggtgatgtc cttgatcagg ccactgcggc tcgacagcag gcggtacatc tcaaaagtct
      901 tttcctgcaa cagttgcacc ttgtttttgc gtagccgcac gatgaacttg ttgagcacgc
      961 tggcaatcga atcgcactcc tcgataaagt ctgccttgtc tttcgacaca ttgtgcttct
     1021 cgaacagctt ttcgatttcg acctcaatgt cgccaatgcg cttggtcaaa gccaaaatgt
     1081 cttcttccac cagccgcagt tgctcggttt tgcgcccaat ctgggttgaa cagctttcca
     1141 tcacgccctg cagttcatcg aatagatcac gctcggtgcc tgacaagccg ccactttgcc
     1201 cgacgccttc aagctggcgc acttgcaccg ccaggctgcg ggcttcgtca agcagcgcct
     1261 tgagcagaaa gacatcgctg ccctccagcg actccatgcg gttgagcaca cgggcggcat
     1321 cgcggtcgga caggtccagt accttggcga cagccgccga ctggtcgccc tccttgagta
     1381 gccggtaaat ccgccgctcc agctctgcca tacgttccgg ggacaatttt tcggtgtaaa
     1441 tcggctccgg gtcctccacc acacgcacaa tgcggcgcgc cagagctgcg gcgttttcct
     1501 tgatcgcctc gccagtggcg gattcgcgct caagctcaat ctgcccccgt atgccggcaa
     1561 ataacttgcc tgcaatgctg aagggcaggg ctttttcggc caagctgcga atttcgctct
     1621 ccacctgagc cagccggttg gcggcctgca cgcgcttctt ttcttgctcg cgcatcgttt
     1681 ctcgtgattc cggtgcgatc tgaaaagttg cctcaaaacg ctttttggcc tcatccaact
     1741 gccccttgaa gccgtccagt tcggcgcgta agctatcgcg ctcatggtgc ttgcgggtca
     1801 gcttgctttt ttcacgcttg agttcgcttt gcttgaattc cagatcctca tcagaaattt
     1861 cgacaaagcc tttacgttcc tcctgcttga tgtagatgat gtcggcagcc agccggttga
     1921 tgtgcccaat gcccagcgca gcctccagcg acgatttcaa gcgcatttcc gaatggtcgt
     1981 cggctgcgat ttcctgaatt ttttcaccat caaagaagaa gaactgggtg atgcccggcg
     2041 gaatggcggc gcggataaag ttttgccaga tttcctggtt ctgcaccgat acccgcttgc
     2101 cgtcacgcac caccaccagc cgctcggtca ggtcgcgcgc tttgggttcg ttgaccgcgc
     2161 ccgccgtcca ggcgcgtttg accaccagtt ccgatgcatc gtccatctcg atcaccagct
     2221 caaacgcgac gttggcattg cctttggctt tctcgcgccg gttgatgttg cgataaataa
     2281 aacaggtaat cccccggctt tgccggggcg acagtagggg ttttac
//
LOCUS       DAOSLL010000678         2325 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_39788_length_2325_cov_3.468282, whole genome shotgun sequence.
ACCESSION   DAOSLL010000678 DAOSLL010000000
VERSION     DAOSLL010000678.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39788_length_2325_cov_3.468282"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="PLB25_20780"
     CDS             complement(<1..222)
                     /locus_tag="PLB25_20780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006498190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HOE44042.1"
                     /translation="MMTFSLAVRNLLRNRRRSLATLLAMAIGSTSILLFGGFSANVNY
                     ELHTRHVQKGGHLQIQHRDFYLYGSGNPTA"
     gene            complement(219..878)
                     /locus_tag="PLB25_20785"
     CDS             complement(219..878)
                     /locus_tag="PLB25_20785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009551257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HOE44043.1"
                     /translation="MDDVSVPVIKGVNVLVRPACFTVLLGPSGSGKTTLLNMIGCIDK
                     PDKGEIIVSDCKVGELTDDELSDFRAKNIGFIFQNFNLLPVLSAYENIEYPLLLAGVP
                     EKTRASRVPKLLEAVGLSDKAKNLPGQLSGGQRQRVAIARALARKPKLVIADEPTANL
                     DSQTGAAILALMRSMVERYHISFIFSSHDREVIKAADDTIFLKDGVVHGIRRKGREIA
                     S"
     gene            1659..1823
                     /locus_tag="PLB25_20790"
     CDS             1659..1823
                     /locus_tag="PLB25_20790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44044.1"
                     /translation="MSVNGTSGYLFGLLDHFWQRHELHIEKASKNYALEAFGLSDGAD
                     DRNRQNAVTR"
BASE COUNT          554 a          636 c          595 g          540 t
ORIGIN      
        1 agcggttgga ttgccgctgc cataaaggta aaagtcacga tgctgaattt gcagatgtcc
       61 ccctttttgt acgtgtctcg tatgcaactc gtaattcaca ttagcgctga aaccgccaaa
      121 caacaatatc gaagtcgaac caatcgccat tgccagcagt gtggccagag acctgcgccg
      181 gttgcgcaac aggttgcgta ccgccaaaga gaaagtcatc atgacgcgat ctcccggccc
      241 ttgcgccgaa tgccgtgaac cacgccgtct ttcaagaaaa tggtgtcgtc tgcagccttg
      301 atgacttcgc ggtcatggga cgaaaaaatg aacgaaatat ggtaacgctc caccatgctg
      361 cgcatcaggg caagaatggc agcaccggtt tggctgtcca ggttggcggt gggttcgtcg
      421 gcaatcacca gcttgggctt gcgcgccagc gcacgggcga tggccacgcg ttgacgctgg
      481 ccacccgaca actggccagg caagtttttg gctttgtcgc tcaagccgac ggcttcgagc
      541 agtttgggca cgcggctggc gcgtgttttt tcaggcaccc cggccagcag caacgggtac
      601 tcaatgtttt cgtaggcgct caacaccggc aacaggttga agttctgaaa gataaaacca
      661 atgtttttgg cacgaaagtc tgacagttcg tcgtccgtca actcgcccac cttgcagtcc
      721 gacacgatga tttcgccctt gtcgggcttg tcgatgcagc caatcatgtt gagcaatgtg
      781 gtcttgccgc tgccagacgg accgagcagc acggtaaaac aggcaggacg caccaatacg
      841 ttaacgccct tgatcaccgg cacactgaca tcgtccatgc ggtaggtgcg ctcaacctgg
      901 gccaacacag caacaggacc ttgcggtggt ggtgcaccga ccgtgctctg agctccatcg
      961 tgttgcactg agtgttcagt catcgttcac ctttgttgag ccaagttcga ctgcctgagc
     1021 caatattacc gcaacaggtg cctggctaag cgccagtcag cgcgggtttt tcaacattaa
     1081 cgcgacgtga attgactgcc cctgctgcgc cttgaagcct gtctcggcaa cgatacacgt
     1141 cctggttttg gcatgagcct gctcgcggct ggccggttga cctggccgca ttggccccac
     1201 catcaagcga atcgccgaag tcacgccatg tcaaccaagg cgcatccggt gcggatgcgg
     1261 tcaactttga ggccaaaagg agagaaattg cggaaaaggg ggtcagtcat tcgctatagc
     1321 cgcaatttgg ctagcagttt gctggaccta caaatcgtat aaaaacaaca atttacattt
     1381 tcaagcagat gatttttcgg atattgcgaa ttgattaatc aaattgccta actttcatgg
     1441 ctttggagct atacgaatca accaattaac agtccgacag cggcctagcg ggatagccgg
     1501 gggcagacta cggtttgacc tgtacaaaca aaaacgatcc cattggaaag acaaccggca
     1561 ctgcccgctg gcaacatcgg tccaatctgt catatatgcg aaataagcct ctagcccttg
     1621 tggcataagc gctacaagct attgaatatg aagcattatt gagcgtcaac ggcaccagcg
     1681 ggtacctgtt cggcctgctg gatcatttct ggcaacgcca tgagttacac attgaaaagg
     1741 cctccaagaa ttacgccttg gaggcctttg gtctatctga tggtgccgat gaccggaatc
     1801 gccaaaatgc cgtaactcgt tgaatttcaa tgttttatca catgcaaaaa ctcggttcgt
     1861 tcccccattt gttcccccgg tttgcctttg atacccccct gaaacagggt taaacgatat
     1921 aaaccgtttc gatcagtttc aggcaaaaaa tattttcaga cctgtctgtt ttgattcacc
     1981 agatcaccgc gatgtttacc agcgccagtc agtccactac cagttcattg atgcccggca
     2041 tgttgatctt gcgtggcacg gtgccagcga atccccgacg ttccgggcat ttgtttttca
     2101 taacggttac gaaaaggcta agcggtgtgc gtcagcccta tatcggcctg ataccaccag
     2161 cgccggtatc actgccacca gaacggctca gggtgcattg aatcagccct gcataggcca
     2221 ttgccccagc aaagtgattc ggctctgcct ggcgcatcta tgggccaatc ccataaataa
     2281 aacaggtaat cccccggctt tgccggggcg acagtagggg tttta
//
LOCUS       DAOSLL010000679         2283 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_40743_length_2283_cov_3.302962, whole genome shotgun sequence.
ACCESSION   DAOSLL010000679 DAOSLL010000000
VERSION     DAOSLL010000679.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40743_length_2283_cov_3.302962"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="PLB25_20795"
     CDS             <1..515
                     /locus_tag="PLB25_20795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-24"
                     /protein_id="HOE44045.1"
                     /translation="QASRTAARTDTLGNPVLLTQQNRARWDHLLIRRGLAALDRAPSL
                     AQAPGSYQLQAAIAACHACAPTAEDTDWARIAALYAMLMRVAPSPVVELNRAVAVSLH
                     QGPAAGLAIVESLLQDQAFQRYHLLHAVHGDLLQKMGLRNEARAALNRAAALAGNDKE
                     RALLVQRAQE"
     gene            531..860
                     /locus_tag="PLB25_20800"
                     /pseudo
     CDS             531..860
                     /locus_tag="PLB25_20800"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
     gene            complement(1210..1671)
                     /locus_tag="PLB25_20805"
     CDS             complement(1210..1671)
                     /locus_tag="PLB25_20805"
                     /inference="COORDINATES: protein motif:HMM:NF025032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HOE44046.1"
                     /translation="MTNSQLLICFNRTLVRVTCALLCFLGARGALAADLTTDVVGSWK
                     LVSHTVTMSGSTFDSHAALLQQRPCAAKIRYEVKADGNYRLDASASDCDEKYRTIQEK
                     LYSKTKWRLEGSKITISATNFAVGQSYSVTVSGNRMTWVGTEGQGTMVLQR"
     gene            2058..>2283
                     /locus_tag="PLB25_20810"
     CDS             2058..>2283
                     /locus_tag="PLB25_20810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011548505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44047.1"
                     /translation="MKHIMKQMQLPSGGRRSGLPMVIDDDWSIEQVVAVAELLEDLLH
                     VIRSRYQGKLYAYLKATRITEFEIDGPNDNE"
BASE COUNT          499 a          600 c          671 g          513 t
ORIGIN      
        1 tacaggcatc acgcacggcg gcgcgcactg atacactagg caacccggtt ctgctcacgc
       61 agcagaaccg cgcgcgctgg gatcatttgc tgatccgccg gggtctggcc gcactggatc
      121 gcgccccaag cttggcgcaa gcaccgggca gttaccagct tcaagccgcc attgccgcct
      181 gccatgcctg cgctccgaca gccgaagaca ccgactgggc gcgtattgcc gcgctctacg
      241 ccatgctcat gcgtgtggcc ccgtcaccgg tggttgagct caaccgcgct gtggctgtgt
      301 ccttgcacca aggaccggcg gcgggccttg ccatcgttga aagcctgttg caagaccagg
      361 cctttcagcg atatcacctg ctgcatgcag tgcatggtga tttgctgcaa aaaatgggtc
      421 tccggaatga agcacgggca gcgctgaacc gggctgcggc gttggcaggg aacgacaaag
      481 agcgtgcctt gttagtgcag cgggctcaag agtaggacca tggcgaattc agaccttggc
      541 tgtttgtatt ggtgctagac gttaaacgtg ttttgctggc agatgctttt gctcaaacct
      601 tggttgaacg cgatgccaac gccacaactc tacaaataga ggttgcccga gtaaaagcgt
      661 ttcgtgtgcc atttctgaca ctggccattg ctaggctggg actttgcgaa cctgatactg
      721 acccgctgga acgaatggtg tcggttgacg cagcaattca aacttttctg ctctgcgcga
      781 acagcatggg ttttggcatt agcctcagca gcggtcaggc catgcgatcg gagccactga
      841 atttgattta ttcggggtga ttggcgaata aaccatttca aatttcccga aagctgccgc
      901 cggggagtga ccgctgttgg ccattggcgg gtgcccactt tgacgaaatc atcggcacaa
      961 agccgccggt cgatctctag ttgccagcgg ctgctatgga gcaggcagat cgcaaatctc
     1021 taagaccgga atcgctgcag tgctgtggta tcacagacca gcacaccatc tgcgcgggcc
     1081 agtccgttcg actcttcata tcgagcgtaa agctcaactg gaaataagag ccgctttggt
     1141 tctgcgggtc cttgaactgt tgcgtaagcg atccagggtg catctcgcca atgcccctca
     1201 gttgaagtgt cagcgctgta agaccatggt gccctgacct tctgtgccaa cccaggtcat
     1261 tcgattgccg gacactgtga ctgagtatga ttgcccgact gcaaagttgg tggccgagat
     1321 tgtgattttg ctgccttcta gcctccactt cgtcttggag tacagctttt cctggatcgt
     1381 cctgtacttc tcgtcgcagt cactcgcgga ggcatccagg cggtaattgc cgtcggcctt
     1441 gacttcatag cggatcttgg ctgcacaagg tcgctgctgc agcagagcgg catgggagtc
     1501 aaatgtgctc ccactcatgg tgacagtgtg agaaacgagc ttccatgaac cgaccacatc
     1561 ggttgtaagg tcggccgcca acgcccccct cgcgccaagg aaacagagta aggcacaagt
     1621 caccctcaca agggtgcggt tgaaacaaat gagaagttgg gagttggtca tggtgttctc
     1681 gaacaaggaa gtgcgactat agagggagat cgggatgctg catggctaag ataacaatgg
     1741 tacaaaagtt gtgcctaatc cagccaactt gtgctggttg agggccaggc aatggtcaac
     1801 gctggccggt ggtcttgggc agtcaatggg gggacaacga tgcctatcaa tcacagtctt
     1861 ccatatttgt gaatcaccct gtagcgttgg tccttatttc ccgttgagac gacaagcgaa
     1921 taactgcttg cccgccacag cacgggtgtg ggcatcaagg gtgcgatggc agtgtcagca
     1981 cggctggcca gcgtgacatg aggttgatcg caggagcgct ctgcacagtt ggctgcgaaa
     2041 cgcaagaaga ttaaatcatg aagcacatca tgaaacagat gcagttgccc tcggggggac
     2101 ggcgatcagg tctgcctatg gtcattgacg atgactggag cattgagcag gtggtggctg
     2161 tggctgagtt gctggaggat ttgctacatg tgattcgcag ccgctatcag ggcaagcttt
     2221 acgcgtattt gaaagctacc cgaatcactg agttcgagat tgatggcccc aacgataacg
     2281 agg
//
LOCUS       DAOSLL010000680         2277 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_40888_length_2277_cov_5.511251, whole genome shotgun sequence.
ACCESSION   DAOSLL010000680 DAOSLL010000000
VERSION     DAOSLL010000680.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40888_length_2277_cov_5.511251"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1201)
                     /locus_tag="PLB25_20815"
     CDS             complement(<1..1201)
                     /locus_tag="PLB25_20815"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HOE44048.1"
                     /translation="MDIRNLKIGVRLGMVFGFILLVSVGMLMGALLSNTASRTTLLDT
                     MQRAKVQQELAQDMHQSLFSSAVSIRNMGLQSTVDAVQKDEAQAKLQRAAYLAAKSKL
                     EGMGLGGPERDLFARLSDIDSKTDTYFKEAVDLAAQFNTEQAGAIITGKIDPLSTQAA
                     AELSAFIGLQKQQTQAAIAQANASNQTTVSVISAIGVLMLLLGMGLAWGLTASITRPL
                     QTAVDAAQRIASGDLVSDIAEDKTIHRTETSCLLNGLLEMRNSLADIVWQVRASAENI
                     SNGANEIAVGNSDLSQRTETQASSLQHTTASMEQLSATVKSNAETAHQANQMASSASS
                     AATNGGVVVSQVVTTMAEITVASHKIADIIGVIDGIAFQTNILALNAAVEAARAGEQG
                     RGFAVVAA"
     gene            complement(1243..1713)
                     /locus_tag="PLB25_20820"
     CDS             complement(1243..1713)
                     /locus_tag="PLB25_20820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HOE44049.1"
                     /translation="MIVSKLIKSIFGATVLGIAMIGASQAADFGTAAEAEAMAKKAVA
                     FIKANGPEKSAEEFTNGKQFKDRDLYISYVDFSGKMLAHGFNPKLVGKGLIDLKDPDG
                     KFMVRMAIDVAKTKGHGWTENYKFKNPTTDKLQEKAMYVERTGDAYIGVGIYKN"
     gene            1991..>2277
                     /locus_tag="PLB25_20825"
     CDS             1991..>2277
                     /locus_tag="PLB25_20825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44050.1"
                     /translation="MVRFLVLPHRFERNLTNSPESGRGDPKNGPFPGTSECDHTDKRK
                     ICQSSKSEFPKPDEPEPKRQHKPSVVRKIFGRSADLRPCKCSWVDQEKVCNP"
BASE COUNT          509 a          651 c          549 g          568 t
ORIGIN      
        1 cggccgccac cacggcaaaa ccacggccct gctcaccggc ccgcgccgct tccaccgccg
       61 cgttgagcgc caggatattg gtctgaaagg caatgccgtc aatgacaccg atgatgtcgg
      121 caatcttgtg tgaggccact gtgatttctg ccatggtggt caccacttgg ctcaccacaa
      181 caccaccatt ggtggctgct gagctggcag aacttgccat ctgattggcc tggtgggctg
      241 tctctgcgtt gctttttacc gtggcactca actgctccat ggacgcagtt gtatgttgca
      301 ggctgctggc ctgggtttca gtccgctggg acaggtcgct gttgccgacc gcgatttcat
      361 tggcaccgtt ggagatgttc tcggcacttg cgcgcacctg ccacacgatg tcagccaggc
      421 tgttgcgcat ttcaagtagt ccgttgagta aacaggatgt ctctgtacgg tgaatggtct
      481 tgtcttctgc aatgtcactt accaggtcgc cgctggctat gcgttgggcc gcatctaccg
      541 ctgtctgcaa tgggcgtgtg atgctggccg tcaagcccca cgccaatccc atcccaagca
      601 acagcataag cacaccaatg gcgctgatca cactcacagt ggtttggttg ctggcgttgg
      661 cctgggctat cgccgcctgc gtctgctgtt tttgcaatcc gataaatgcc gacagttccg
      721 ctgccgcctg ggtggatagc ggatcaatct tgccagtgat gattgcgccg gcctgctcgg
      781 tgttgaactg ggcagcaagg tcaaccgcct ccttgaaata ggtgtcggtc ttgctgtcaa
      841 tgtcggacaa acgtgcgaat aggtcacgct ccggcccacc caaacccata ccctccagct
      901 tggacttggc cgctaaataa gcggcgcgct gaagtttggc ttgggcctcg tctttctgta
      961 cagcatcaac cgtggactgc agccccatgt tgcgaataga caccgcactg ctgaacaatg
     1021 attgatgcat gtcttgcgcc agttcctgct gcactttggc acgctgcatg gtgtccaaca
     1081 acgttgttcg actggcggtg ttgctaagca gcgcacccat caacatgccg actgatacca
     1141 gcaatatgaa gccaaacacc atacccagtc gtacaccgat tttcaggtta cgaatatcca
     1201 taatcaacat cctgacatcg gctaacccgc agtcaacaca attcagttct tgtagattcc
     1261 gacacctatg taggcatcac cagttcgctc cacgtacatc gctttttcct gtaacttgtc
     1321 tgtcgttgga ttcttgaact tgtagttttc tgtccagcca tggcccttgg tcttggcgac
     1381 atcaatcgcc atacgtacca tgaacttgcc gtcggggtct ttcagatcaa tcaggccttt
     1441 gcccaccagt ttgggattaa aaccgtgtgc gagcatcttt ccagaaaaat ccacatagct
     1501 gatgtagaga tcgcggtctt tgaactgctt gccgttggta aattcttcag cagacttttc
     1561 cggaccgttg gctttgatga aggccactgc cttctttgcc atggcttccg cctctgcggc
     1621 tgtgccaaag tcagcagctt ggctagctcc gatcatggca atacccaaaa cggtggcccc
     1681 aaagatggat ttaatcaatt tagacacgat cattttctcc aaaaaacaac aaaaccgaac
     1741 atttgcctga cggtgaacaa tgttgaagac ttgcgaaatc cacgcttaac catcgtccaa
     1801 ctacaaaggt ggtggccttt gcgtcctttg ccgattcatc caaatgagat tcggcgacca
     1861 actccagaac atctcgacac tcactgcact gttttcagcc aataacaagt cgaagtccat
     1921 gtcttgcagt cagcgcacga gcaacctcaa tatcagacta cggaaactcg cctcaacttc
     1981 gatcactacc atggtgcgct ttttggtctt gccgcatcgc ttcgaacgca atttgactaa
     2041 ctcacccgag tcaggtcgag gtgacccaaa aaatggacct ttccccggca ccagtgagtg
     2101 tgatcatacg gacaagcgca aaatttgtca gtcgtccaaa tctgaatttc ctaagccgga
     2161 cgagccggaa ccaaaaaggc aacataaacc ctcggtcgtg cgcaagattt tcggacgttc
     2221 agccgattta cgtccttgca aatgttcttg ggttgaccaa gagaaagttt gtaaccc
//
LOCUS       DAOSLL010000681         2264 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_41219_length_2264_cov_2.288818, whole genome shotgun sequence.
ACCESSION   DAOSLL010000681 DAOSLL010000000
VERSION     DAOSLL010000681.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41219_length_2264_cov_2.288818"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="PLB25_20830"
     CDS             <1..412
                     /locus_tag="PLB25_20830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44051.1"
                     /translation="WSADGMRGHGGVWTKCALPIHINLNIKPVVPMKSLFFYLILSLL
                     IVGCGQREEVETRQVIIAQQQVAQANRAKEEAEKKAAIALANQKAAEEKAAVESSSSQ
                     RIIFGLGVGALLAFFVGVGLGSASRKVAAKASSE"
     gene            471..842
                     /locus_tag="PLB25_20835"
     CDS             471..842
                     /locus_tag="PLB25_20835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44052.1"
                     /translation="MPISSPVIDHDLDEMMVLERVTEVTRFQIHQLEYLVSPGGGLRA
                     WLRVNMLLALLLLIPAMFVVPVVTSLAGQFATLTAYLYQASVNLFYTVLTLIGTVASV
                     LAFISILKFWLGNGGRGSRRR"
     gene            complement(1016..1180)
                     /locus_tag="PLB25_20840"
     CDS             complement(1016..1180)
                     /locus_tag="PLB25_20840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44053.1"
                     /translation="MNQILGSLGIFGILIFIFLVIILPISVYAAQKWAYKCFKELEKL
                     NEKIDRLSPK"
     gene            complement(1365..1652)
                     /locus_tag="PLB25_20845"
     CDS             complement(1365..1652)
                     /locus_tag="PLB25_20845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44054.1"
                     /translation="MANSKVELPATVDAVVRLLIGLVPEAEQARIAYMREDELVTLEI
                     GLGMWTRNNLGLWNGNAALLDATGEAHADDASGVIIRAFWLALRAELPKVH"
     gene            1759..1983
                     /locus_tag="PLB25_20850"
     CDS             1759..1983
                     /locus_tag="PLB25_20850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44055.1"
                     /translation="MIGVVLLVPTLMVVPVLTTLFGSFVTMTTFLRQAAMNLLYAVLS
                     FLAAVASVLVFGYVLKIWLGSGGRGSRGRR"
BASE COUNT          493 a          570 c          616 g          585 t
ORIGIN      
        1 ctggagcgcg gacggcatgc ggggccacgg cggcgtctgg acgaaatgcg cattgcccat
       61 tcacatcaat ctaaacatca aaccggtggt acccatgaaa tcactatttt tttacttgat
      121 cctgtcgctt ctgatcgtcg gttgcggcca gagggaagag gtcgaaactc ggcaagtcat
      181 cattgcccaa cagcaagtag ctcaggcaaa cagggcgaag gaagaagccg aaaaaaaggc
      241 agccatcgct ctggccaacc agaaggctgc tgaggagaaa gctgcggtag aaagttcaag
      301 ttcgcaacgc atcatcttcg gccttggagt tggcgctttg ctggccttct tcgtcggcgt
      361 tggtcttggg tcggcatcga ggaaggtcgc cgcgaaggca tccagtgagt gactattcgg
      421 accgcccaag acgatccttg ttgaccctcg ttcgcgatgc atggcaaccc atgccgattt
      481 catcgccggt aattgaccat gacttggatg aaatgatggt gctggagcga gtcacggaag
      541 tcactcgatt tcaaattcat caacttgagt acctggtctc gcctggcggt ggccttcgtg
      601 catggctgcg cgtgaacatg cttctggcgc tgctcttgct cattccggca atgtttgtgg
      661 tgccggtggt cacgtcgctg gctggccagt tcgccacact caccgcgtac ctgtatcagg
      721 cgtcggtgaa cctgttctac acggtgctca ccttgattgg cacggtggca tcagtcctgg
      781 cgtttatttc gatcttgaaa ttttggttgg gaaatggtgg gcgcggttcg aggcggaggt
      841 gaggcctcgg tagcgggagg gatggcgctg aatgtgtgct caagacggca accgggtagt
      901 caagtcttcg acgtcaccgg cgggtctcga tccaaagcgg tcgaaaaaag caaagctaaa
      961 ccgaagctgg ccagcgatca gcttgagcga tttgttggat ttttaatagg cgaacttatt
     1021 ttggcgatag tcgatcgatt ttttcattta gcttttcaag ctccttgaaa catttatatg
     1081 cccatttttg ggcagcataa actgagatgg gaagaatgat tactaaaaaa ataaagataa
     1141 gaattccaaa aattccgaga ctgcctaaga tttggttcat gttgcactcc tgtgaaaaga
     1201 gattgtttct tcaatgtaac gtatatcgca aaacttgatg gatactcttc ctgctgcggt
     1261 ttaagcttgt cggagtgaac tgccgacgag gctttgtctg caaaatactc ccacgcttaa
     1321 ctgttacatc ttggccgaga ttgccggggt gaacttgacc tacctcaatg aaccttcggc
     1381 aactcagcac gtagcgccaa ccaaaacgcc cgaatgatca cgcccgaggc atcgtcggca
     1441 tgtgcttcac cagttgcatc aagcagcgcc gcattaccgt tccagagtcc caagttgttc
     1501 ctggtccaca tgcctagccc aatctctagg gtgaccaact catcttccct catgtaggcg
     1561 attcttgcct gttcggcttc aggaacaaga cctatcagca gtcggacaac tgcatcgact
     1621 gttgcaggca attcgacctt gctattggcc atcgcgctct gccttgcggt agtcccaagg
     1681 tgagctcgca caggtctgcg ctcagcatta ccacctgttg cgcgactaca cctgcgtgga
     1741 tttatccgcc tgaacgtgat gatcggcgtg gtccttctgg tgccgaccct gatggttgtg
     1801 cctgtattga ccacgctgtt cggatcgttc gtgaccatga caacgtttct gaggcaggca
     1861 gcaatgaact tgctctatgc ggtgctgtcg ttcctagcgg cagttgcttc agtgttggtg
     1921 tttggatatg tcttgaaaat ttggttgggc agcggcgggc gtggttcgag aggtagaagg
     1981 tgaggcctta cttctttgaa tgcgaagtca gatacttgag tgactactgt gcttcgtcaa
     2041 cactgaggta cctgacatct gcgaccctga caaagacaga tttcccttga ctacccctga
     2101 gtgagaattt tttgcgtcat ctcatttcca ctatctttcg gtggatcacg aaatacttca
     2161 tcagtttcgt cgtcattctg gcggtcctgg ttgtcggaaa gtggttctcc acgcaactgc
     2221 aagcggctgt cgcggttcgg gcccaggtgg ccgcgctcca gagt
//
LOCUS       DAOSLL010000682         2224 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_42246_length_2224_cov_3.318119, whole genome shotgun sequence.
ACCESSION   DAOSLL010000682 DAOSLL010000000
VERSION     DAOSLL010000682.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42246_length_2224_cov_3.318119"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="PLB25_20855"
     CDS             complement(<1..790)
                     /locus_tag="PLB25_20855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HOE44056.1"
                     /translation="MTADLSVFWDLLIGPFAEFAFMRRALVATLALSLSAAPLGVFLT
                     LRRMSLLGDALSHAVLPGVAIGFMLAGLSLSAMALGGVIAGMLVAGIAGLVSKFTTLR
                     EDASLAAIYLVALALGVTLIASHGTQLDLLHILFGSALGVDNAGLLLVAAVASVSLLA
                     LAVLYRGLVLETFDPVFLNASRKTGRGTWLWQQGFLMLVVINLVAGFQTLGTLMAVGL
                     MMLPAVSSRLWHDTLPAQLINASVQAMLAGFAGLLLSYHFDTPSG"
     gene            complement(1037..1828)
                     /locus_tag="PLB25_20860"
     CDS             complement(1037..1828)
                     /locus_tag="PLB25_20860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HOE44057.1"
                     /translation="MIKLRNLTVSYRHHPALHHINGEFAPGSLTAVIGPNGSGKSTLL
                     KSIVGLLPIEGGRSGGAMSVATERQRMAYLPQQSEIDRSFPIDVRDCVLLGCWGMAGA
                     FGNISPAMLTRVDAALHTVGLQGFERRAVGSLSSGQFQRVMFARMLVQDAQLILLDEP
                     FNAVDAKTTARLLALVRQWHQEQRTVIAVLHDDAQVRQYFPQTLLLARELVAWGPTGD
                     VLTEANLQRARAMAEAWDETAEVCELDITPDSHHHPHAPAAHPVN"
     gene            complement(1917..2063)
                     /locus_tag="PLB25_20865"
     CDS             complement(1917..2063)
                     /locus_tag="PLB25_20865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44058.1"
                     /translation="MQTRLSKSPLLTKSVLAWPAWLRLLAVLPALLLLWLAVLWASAE
                     AVPL"
BASE COUNT          462 a          706 c          688 g          368 t
ORIGIN      
        1 gtcccgacgg tgtgtcaaag tggtaggaca gcagcagccc ggcaaacccg gccagcatgg
       61 cttgcacgct ggcattgatc agttgcgcgg gcagggtgtc gtgccacagg cggcttgaca
      121 ccgcaggcag catcatcagg ccgactgcca tcagcgtgcc cagggtctga aaaccggcca
      181 ccaggttgat caccaccagc atcaagaaac cctgctgcca cagccaggtg ccgcgcccgg
      241 tcttgcggct ggcattcaga aacaccgggt caaaggtttc cagcaccaga ccccggtaca
      301 gcaccgccag cgccagcagg ctgacgctgg caacggctgc caccagcagc agcccggcgt
      361 tgtccacgcc cagggcactg ccaaacagga tgtgcagcaa gtccagttgg gtgccgtggc
      421 tggcaatcag cgtcaccccc agcgccagtg ccaccaggta aatcgcggcc aggctggcat
      481 cttcccgcaa agtggtgaac ttgctgacca gtccggcgat gcccgctacc agcatgccgg
      541 cgatcacgcc acccagtgcc attgccgaca gcgacaggcc cgccagcatg aagccaattg
      601 ccacgcccgg cagcaccgca tggctgagcg catcacccag caggctcatg cgccgcagcg
      661 tcaaaaagac acccagggga gctgcgctga gcgacagcgc cagggtggcc accagcgccc
      721 ggcgcatgaa ggcaaattcg gcaaacgggc caatcagcag gtcccaaaaa acgctcaggt
      781 cggccgtcat ggcaggcggc accgtgtctt gaaagtcgct gccagggaca agcgtgaaat
      841 gcaagaaata gtctgcgcaa aacccccgac atggccgcaa aggcgggatt gccgatgcgc
      901 cagattgccg cccttgcagc catggcgacg atcctgcatc tggcgggggc gggtcatggt
      961 cttgcatggc gcatcagtca acacctttgt cctatcgcga cgatctttca tcaggcgggg
     1021 tccggtcatg gcaggctcag ttgaccggat gcgctgctgg cgcgtgcggg tggtgatggc
     1081 tgtctggcgt gatgtcaagt tcacacactt cggcagtctc gtcccaggct tcggccatgg
     1141 cacgggcgcg ctgcaggttg gcctcggtca gcacgtcgcc ggttggcccc caggcgacca
     1201 gttcgcgcgc cagcagcagg gtttgcggaa aatactggcg cacctgcgcg tcatcgtgca
     1261 gcacggcaat cacggtgcgt tgttcctgat gccactggcg caccagcgcc agcagccggg
     1321 cggtggtctt ggcatcaacc gcgttgaatg gttcatctag caagatcagt tgcgcatcct
     1381 gcaccagcat gcgggcaaac atgacccgct ggaattgccc gctcgacagc gaaccgaccg
     1441 cgcggcgctc aaacccttgc aggccaacgg tgtgcaaggc ggcatcgaca cgcgtcagca
     1501 tggccgggct gatattgcca aatgcgccag ccatacccca gcaacccagc agcacgcagt
     1561 cgcgcacatc aatgggaaaa ctgcggtcaa tctcgctttg ctgtggcagg taggccatgc
     1621 gctggcgctc ggtggcaacg ctcatagccc cgccagagcg gccaccttca atgggcagca
     1681 gaccgacaat gcttttgagc agggtgctct tgcccgagcc attgggcccg atcaccgccg
     1741 tcagcgaccc cggcgcaaac tcgccattga tgtggtgcag ggcagggtga tggcggtagc
     1801 tgaccgtcag gttgcgcagc ttgatcatgc cggattcctc atttgaccgg gccagggtgg
     1861 gctggcgcgg ctcggtaggg cggcgcaggc cgcaggcgca tcggttggcc acaggctcac
     1921 agtggcaccg cttccgcgct ggcccaaagc accgccagcc acagcagcag caatgcaggc
     1981 aacaccgcca gcaagcgcag ccaggctggc caggccagca cgcttttggt caacaggggt
     2041 gatttggaaa ggcgggtttg catgaacaag gggcggtaaa aaaatgggca atagggcagg
     2101 ttacagcggt cataatgcaa ctcgattgcg gtatagttta atgcaacttg attgcgttaa
     2161 ttgtctaaat tcagaaattt gtcattccct gccatggtct tagccacatc caccgttgcc
     2221 acac
//
LOCUS       DAOSLL010000683         2217 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_42408_length_2217_cov_4.729880, whole genome shotgun sequence.
ACCESSION   DAOSLL010000683 DAOSLL010000000
VERSION     DAOSLL010000683.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42408_length_2217_cov_4.729880"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            9..803
                     /locus_tag="PLB25_20870"
     CDS             9..803
                     /locus_tag="PLB25_20870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013899307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HOE44059.1"
                     /translation="MAVQTVSAQVRELERSLGYALLKPAGRGLVLTEAGQAAMQQADQ
                     IFQLGEGLPARVRDAASAPTVRLAVGICDGLPKLVVHRLLLPIITEPNLRLLCHEGEL
                     DDLLGDLALHRLDLVLSDRPAPSNPNIKLYSHAMGSSGIAWYATPALVAATSQDFPQS
                     LANLPMLMPTAHAAVRARLDHWFEQRAMRPRIVGEFADSALLKTFGASGMGVFPAAEW
                     AHEDLLAHYAVQRLGSCDGVTEHFFAIGTEKKVQHPLVQRLLQPAP"
     gene            complement(869..>2217)
                     /gene="lon"
                     /locus_tag="PLB25_20875"
     CDS             complement(869..>2217)
                     /gene="lon"
                     /locus_tag="PLB25_20875"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012747622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HOE44060.1"
                     /translation="PGVGKTSLGQSVAKATGRKYVRVALGGMRDEAEIRGHRRTYIGA
                     MPGKVLQSLAKVGTRNPLFLLDEIDKLGTDFRGDPSSALLEVLDPEQNHKFGDHYVEV
                     DFDLSDVMFVATSNSMNIPPALLDRMEVIRLSGYTEDEKTNIALKYLLPKQLKNNGVK
                     EEELCVTEDAVRGIVRYYTREAGVRSLEREISKICRKVVKALQLKKMTAQVNVNGDNL
                     NDFLGVRKYDYGRAEKKNQVGQVVGLAWTEVGGDLLTIEAAMMPGKGVITRTGSLGDV
                     MKESVEAARTVVRSRSHRLGIKDEFFEKKDIHIHVPDGATPKDGPSAGAAMTTAFVSA
                     MTGIPVRCDVAMTGEITLRGEVTAIGGLKEKLLAALRGDIKTVLIPEENAKDLQDIPE
                     NVKNGLEIVPVRWIDKVLEIALVHMPTPLPEDDPVAVAATPEVSIAGTNLTESVKH"
BASE COUNT          450 a          666 c          600 g          501 t
ORIGIN      
        1 agctcgacat ggcggtgcaa accgtgagtg cgcaggtgcg tgaactggag cgctcgctgg
       61 gctatgcgct gctcaaacct gccgggcgcg ggctggtgct gaccgaggcg ggacaagccg
      121 ccatgcagca ggccgaccag atttttcagc ttggcgaggg tctgccagcg cgggtgcgtg
      181 acgcggcgag cgcgccaacc gtgcggctgg cggtgggcat ctgcgatggg ctgcccaaac
      241 tggtggtgca tcggctgttg ctgccgatca tcaccgaacc caatttgcgc ttgctgtgtc
      301 atgaaggcga attggacgat ttgctgggtg atctggcctt gcaccggctc gatctggtgt
      361 tatcagaccg gcccgcaccc agcaacccca atatcaagct ctacagccat gccatggggt
      421 cgtcgggcat cgcctggtat gcaacccccg ccttggtggc tgccaccagc caggactttc
      481 cgcaaagcct ggccaacctg cctatgctaa tgccgaccgc gcacgctgcc gtgcgtgccc
      541 ggctggatca ttggtttgag caacgcgcca tgcggccgcg cattgtgggc gagtttgccg
      601 acagcgcgct gctaaagacc tttggtgcca gtggcatggg cgttttcccg gcagccgagt
      661 gggcgcatga agacctgttg gcccattacg cggtgcagcg gctcggcagt tgtgacggtg
      721 tcaccgagca cttttttgcg attggcaccg aaaaaaaagt gcagcatccg ctggtgcagc
      781 gcttgctgca gccagcgcct tgattgctgt atggactgtg gaaattgttc ccgcttgccg
      841 gacttgaatc gtacccagtc aaatctcgtc agtgtttgac cgattcagtc aaattggttc
      901 cggctatcga gacctctggt gttgcagcga cggcgacagg gtcgtcctca ggcaaaggcg
      961 ttggcatatg aaccaacgcg atctcaagta ccttgtcaat ccagcgcacc ggcacgattt
     1021 ccaggccatt tttcacgttt tcaggaatat cctgcaaatc cttggcgttc tcctcgggaa
     1081 tcaacaccgt cttgatgtcc ccacgcaaag ccgccagcaa tttttccttt aacccaccaa
     1141 tagcggtgac ctcaccacgc aaggtaatct cgcccgtcat tgccacgtca caacgcaccg
     1201 ggatccccgt catggcagag acaaaggcag ttgtcatggc cgcgccagcg ctgggaccat
     1261 ctttgggcgt tgcaccatca ggtacatgaa tgtggatatc tttcttctcg aaaaattcgt
     1321 ccttgatgcc caatcgatgg gaccgactgc gtaccaccgt gcgcgccgct tccaccgact
     1381 ccttcatcac atcgcccaaa gaaccggttc gggtgatgac acctttgccc ggcatcatgg
     1441 cagcttcaat ggtcagcaaa tcaccaccca cctcggtcca ggccagacca accacctggc
     1501 cgacttgatt tttcttttcg gcccggccat agtcatactt gcgcacgccc agaaaatcat
     1561 tcagattgtc accattgaca ttcacctgcg ccgtcatttt cttgagctgc agagccttga
     1621 ccaccttgcg gcaaattttg gatatctcgc gttcaagtga acgaaccccg gcctcacggg
     1681 tgtagtaacg aacaataccg cgcaccgcgt cttcggtcac gcaaagctct tcttctttga
     1741 caccgttgtt tttgagttgc ttgggcagca agtacttcag ggcaatgttg gtcttttcgt
     1801 cctctgtgta gcctgagagt cgaatcacct ccatccgatc aagcaaggct ggcggaatgt
     1861 tcatcgagtt cgatgtcgca acaaacatca cgtcgctcaa gtcaaaatca acctcgacgt
     1921 agtgatcgcc aaatttgtga ttttgctcag ggtccagcac ctccagcaat gcgcttgaag
     1981 ggtcgccacg gaagtcagtg cccagcttgt cgatttcatc gagcaaaaac aatgggttgc
     2041 gggtgcccac cttggccaga ctttgcaata ccttgccagg catggcaccg atgtaagtcc
     2101 ggcgatggcc gcgaatttcc gcttcatcgc gcatgccacc aagcgccaca cgcacgtatt
     2161 tccgccctgt ggctttggcg acagattgac ccaaggatgt tttgccaaca ccaggag
//
LOCUS       DAOSLL010000684         2210 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_42585_length_2210_cov_4.165197, whole genome shotgun sequence.
ACCESSION   DAOSLL010000684 DAOSLL010000000
VERSION     DAOSLL010000684.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2210
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42585_length_2210_cov_4.165197"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..587)
                     /locus_tag="PLB25_20880"
     CDS             complement(<1..587)
                     /locus_tag="PLB25_20880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44061.1"
                     /translation="MTTPGSEPFQRDVTLAKELVGKRFGSKQLALIRPVLRAVNVRLY
                     HSSMHYDDLNTVTVRHLDEPLISGVPPWQLMFTGEGETGTNNLFFIACEAHLFACIQA
                     LHAAADNLAHVLYYGLGWNLEGKPPKGKVSIKTIRGRLLQLGQSRHELRPIYGPLDVL
                     VNSHQFKLLEDAANHIKHHGGLHASVKWEPNPEKP"
     gene            complement(766..1521)
                     /locus_tag="PLB25_20885"
     CDS             complement(766..1521)
                     /locus_tag="PLB25_20885"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HOE44062.1"
                     /translation="MPQHPQPPWWTTDCDNWWAGSASPSLTIQTAIADYLSLIDDRIT
                     LLRETNATLESIAQALFKSWFVDFDPVRAKMEGRAPEGMDEATAALFPDSFEESELGL
                     VPKGWRVLRLGDATERITKGTTPTTLKRPFVEKGINFIKAESMTGDGGFIPEKFAFID
                     EETHELLRRCQLQDGDVLISIAGTIGRIAAMSKDFLPANTNQAVALIRPLARIFPSGL
                     INRFLRLSESQQRMGEKVVQAVQANLSLGTLSN"
     gene            complement(1531..1869)
                     /locus_tag="PLB25_20890"
     CDS             complement(1531..1869)
                     /locus_tag="PLB25_20890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019561411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HOE44063.1"
                     /translation="MGAFATRQVVLLPFPFSDLTASKLRPALVLASAGKGDWVLCQIT
                     SNPYADPSAVLIEDTDFAQGSLQRVSYARTSKLFTAHESLFQRAIGQLKPECHANVVR
                     VVVDSLQAGR"
     gene            complement(1842..2072)
                     /locus_tag="PLB25_20895"
     CDS             complement(1842..2072)
                     /locus_tag="PLB25_20895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44064.1"
                     /translation="MMQTYEAVLQPDGHLQFLDLVAEAHLTPRRVLVTFTEEFVPEDT
                     ALCGATLSESALAQDWKRVEEDVAWAHLQPAK"
BASE COUNT          465 a          615 c          609 g          521 t
ORIGIN      
        1 tatggttttt ccggatttgg ttcccacttg acacttgcgt gaagcccgcc atgatgcttg
       61 atgtggttgg ctgcgtcttc cagtaatttg aactggtgac tgttcacaag cacatcaagc
      121 ggcccataga taggtctcaa ctcatgacgg gattggccaa gctgcagcaa gcggccccga
      181 atcgttttga tcgacacctt ccctttggga ggtttgcctt ccagattcca acccaggcca
      241 tagtacaaaa catgcgccaa gttgtcggct gccgcatgta gcgcctgaat gcatgcgaat
      301 aggtgggctt cgcacgcgat gaaaaacagg ttgttggtcc ctgtctcacc ttcaccggtg
      361 aacatcagtt gccagggcgg taccccgctt attagtggtt cgtcgaggtg gcgaactgtg
      421 acggtgttga gatcgtcgta atgcatcgaa gagtgataca gccgcacatt cacagcgcga
      481 agcactgggc gaatcaatgc gagctgcttc gagccaaagc gcttgcccac cagctccttg
      541 gcgagcgtga catcccgttg gaagggttca ctgccgggcg tggtcatacc attgcttcgg
      601 cttctgggat gcgcaactgg ccggagatca gtcgcagcag caaggtgtcg cgcagggtgg
      661 ctagggttct gactctttga tcatttgccg tccagctcgc atcaatttga gccagtccag
      721 actgatacag ctcgcacaca acagattcag gcggaacaac catttttaat tggacaaggt
      781 tccgagactc aaattggcct gcaccgcctg aaccactttt tcacccattc tttgctgcga
      841 ctcgcttagt cgaagaaaac ggttgattag cccactcggg aaaattcgag caagaggccg
      901 aatcagggct actgcttgat tggtatttgc cggcaggaag tcctttgaca ttgcggcaat
      961 gcgtccaatc gttcccgcaa tggagatgag gacatcgcca tcttgaagct gacaacggcg
     1021 cagaagctca tgcgtttctt cgtctatgaa tgcaaatttt tctggaatga agccgccatc
     1081 acccgtcatg gattcggctt ttatgaaatt gatacccttc tcgacgaagg ggcgcttcag
     1141 ggtagtagga gtagtgccct tggtaattcg ctccgttgcg tcaccaagtc gtagtacccg
     1201 ccaccccttc ggcaccaacc ccagctccga ttcctcgaag ctgtcgggaa acagcgccgc
     1261 cgtggcttca tccatgccct cgggggcgcg gccttccatc ttggcgcgaa ccgggtcgaa
     1321 atcgacaaac cacgacttga acagcgcctg ggcgatggat tccagcgtgg cgttggtttc
     1381 gcgcaggagg gtgatgcggt cgtctatcaa cgacaagtag tcagcgattg ccgtttgaat
     1441 tgtcagcgac ggactcgctg acccggccca ccagttgtcg cagtccgtcg tccaccatgg
     1501 cggttgaggg tgttggggca tctctggtac tcatcgtcca gcctgcagcg aatcaaccac
     1561 cacccgcacc acgttggcat ggcattcggg cttgagctga cctatcgcac gctgaaacag
     1621 gctttcatgg gctgtgaata gcttgctggt tcgagcgtaa ctcacgcgct gcaggcttcc
     1681 ttgcgcgaaa tctgtatctt cgatgaggac agcggatggg tcagcatagg ggttgctggt
     1741 gatttgacac agcacccagt cgcctttgcc agcactggcc aacaccagag cggggcgtag
     1801 tttgctggca gtgaggtctg aaaacggaaa cggcaataaa actacttggc gggttgcaaa
     1861 tgcgcccatg cgacatcctc ctccacacgc ttccaatctt gtgcaagggc ggattcactc
     1921 agggtggcac cgcatagcgc ggtgtcttcc gggacaaatt cttcggtgaa cgtgaccagc
     1981 actcgccggg gggtcaggtg tgcctcggcc accaagtcca gaaattgaag atgcccgtcg
     2041 ggctgcaaaa cagcttcata agtttgcatc atgacttgga ctcctttgtg ttggttcaga
     2101 actcatatcc taaccctccc agcttttgcc ggatcaactg atccaactcc gcccccttgg
     2161 ccctctgctc gcccagcttt tcggtgagct gctgcatttt ggtggcgaag
//
LOCUS       DAOSLL010000685         2209 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_42617_length_2209_cov_3.741876, whole genome shotgun sequence.
ACCESSION   DAOSLL010000685 DAOSLL010000000
VERSION     DAOSLL010000685.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42617_length_2209_cov_3.741876"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..311
                     /locus_tag="PLB25_20900"
     CDS             54..311
                     /locus_tag="PLB25_20900"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HOE44065.1"
                     /translation="MAERAAMSRSAFASRFKEVVGLAPADYLTEWRLTLTKKWLQQGR
                     AVKLIAPDVGYANASALSRVFSQRTGMSTRQWLAQSQGTGR"
     gene            complement(388..558)
                     /locus_tag="PLB25_20905"
     CDS             complement(388..558)
                     /locus_tag="PLB25_20905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44066.1"
                     /translation="MVVIAPVHALMHDQAAALHEGGVSAALSNSALSMQQTSQVKRHL
                     IQGEITLEHGRA"
     gene            1170..2135
                     /locus_tag="PLB25_20910"
     CDS             1170..2135
                     /locus_tag="PLB25_20910"
                     /inference="COORDINATES: protein motif:HMM:NF024799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HOE44067.1"
                     /translation="MSMVSQCSHLAVIPLAALWIACAPPAVAQEKKLEITMIFQTEEG
                     FGFFNPVKKGAEDAAALVGVHVEFLCASGSLERYESLLGQALSAKPDGVALTIIDDRA
                     DRDEVCALVKVGIPVIAYTVDDSKGAKGSCRLAFIGQDFVATGYLIGKRMIAEADIKG
                     GDLVLTPVDSPDAVYAILRQRGVDKAMKEVGARTEIPGAGFKDAEILASQMKYLGSHP
                     NVKAIIGIGLPQVEMAIKATESIKRPIPIGGFDLSQSMIDGIESGKVVATVDQQPYTQ
                     GFHSVMQLIHKVHYGLDPSDMKTGGDGLVDKTNYKLSEAGLGKRR"
BASE COUNT          472 a          594 c          658 g          485 t
ORIGIN      
        1 tagtccatcg ctgcttgtga tgtcaagttc cacacaaaac ggcatagagc cgtttggcag
       61 agcgcgccgc catgtcccgc agtgcatttg ccagccgatt caaagaggtg gtgggcctgg
      121 ccccggcaga ctacctgacc gagtggcgac tgacactcac caaaaaatgg ttacaacagg
      181 gccgggctgt gaagctgatt gcaccagatg tggggtacgc caacgcttca gcgttgtccc
      241 gtgttttttc tcagcgcacg ggcatgtcaa cgcggcagtg gctggcccaa agtcagggca
      301 caggacgtta gcgaagccgc cagtcggctc aagcggcttc gctcaaaaag cgagtcgggt
      361 ggcgccaggt cgggtgtcgt gatgcgctca ggcgcggcca tgctccaagg tgatttcgcc
      421 ctggatcaaa tgccgcttga cctgggatgt ctgttgcatc gatagggccg aatttgatag
      481 cgctgcgctg acaccgccct catgcagcgc cgctgcctga tcgtgcatca gcgcatgcac
      541 cggcgcaatc accaccatgc tgcccagccc gacgcgttgg cgggccagcg ccgcaatctg
      601 gcaacacagg cccttgccgc cacactgggc atcagcgaca gcgcgtcgcc tccgacgatc
      661 acctgttcga tgctgttcgc tgagtcaggt gaatgcctgt tggtcaaaaa cctggttgag
      721 aacggatgtg gtggagacag cgtttcgcta tattaaacgg agctgcttgc gcttgtccca
      781 catggtctgg aggcataaaa gacccttaat ctggtggttt ggtctagtga ctgctgtgtg
      841 gttggttttt tgtcctaacg gccacctggt gatcggcggc aagcatgggt tttgagcggc
      901 cggggaaatg gtgttcgacg ggcaggcaag tgtgcatcaa tcccgcaaat tagccacttc
      961 tcgatagaaa tcgctcgaaa gtgcttgttg ccagtatcag acttttccca gaagtcactc
     1021 agatttcagg agagcaactt cagatttgga tgactgacaa gtattgcgcg cttctataaa
     1081 gtgacacctg ctgcggtggg gaaaatagcg tgactttcct aacggtctcg gttcgaggcg
     1141 cttgttggtg ccgcatgcag gagtcaacca tgtcaatggt cagtcaatgc agccaccttg
     1201 cagtcatacc gcttgcggcc ctgtggatcg cctgcgcccc tcccgctgtt gcccaggaaa
     1261 agaagcttga aatcaccatg attttccaga ccgaagaggg cttcgggttc tttaatccag
     1321 tcaagaaagg cgccgaagat gctgcagcgt tggtcggtgt acatgtcgaa ttcctgtgtg
     1381 ccagtggtag tctggaacgc tacgaatcgc tgttgggaca agccttgtcg gcaaagcccg
     1441 atggtgttgc gctcaccatc attgatgacc gggccgaccg tgacgaggtt tgcgcgctgg
     1501 taaaggtcgg catacccgtg attgcctata ccgtggacga ctccaaaggt gccaagggaa
     1561 gctgtcgcct ggcgttcatt ggtcaggact ttgttgccac cggctatctg attggcaagc
     1621 gcatgattgc cgaagccgac atcaagggtg gtgatttggt gctgacgccg gtggattcac
     1681 cggatgccgt gtacgccata ctgcgccagc gcggcgtgga caaggcgatg aaagaagttg
     1741 gagcacgcac cgagattccc ggagcagggt tcaaggatgc cgaaattctg gcaagccaga
     1801 tgaagtatct gggcagtcat cccaacgtca aggccatcat cggcattggc ttgccgcagg
     1861 tggagatggc aatcaaggcc acggaaagca ttaagcggcc cattccgatt ggtggctttg
     1921 atctatctca atccatgatt gatggcatcg agtccggcaa ggttgtggca acggtggacc
     1981 agcagcccta cacccagggt ttccattccg tgatgcaact gatccacaaa gtgcattacg
     2041 gcctcgaccc ttccgacatg aaaaccggtg gcgacggtct ggttgacaag accaactaca
     2101 agctgtccga agccgggctc ggcaaacggc gctagttcgc agatttctgc acaacgcttt
     2161 tgggtcgcca gccgtgaaag ccaagctttt gcgctcggtg caattcagg
//
LOCUS       DAOSLL010000686         2187 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_43194_length_2187_cov_3.582552, whole genome shotgun sequence.
ACCESSION   DAOSLL010000686 DAOSLL010000000
VERSION     DAOSLL010000686.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43194_length_2187_cov_3.582552"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="PLB25_20915"
     CDS             <1..1351
                     /locus_tag="PLB25_20915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HOE44068.1"
                     /translation="RCTEADIDSAVSAARAAFDDKRWCGMSPAARKRVMIKFADQLLV
                     HADELALMETLDMGKPIKYSRAVDVNSAANCIRWYGEAVDKIYDEIAPTASTALALIT
                     REPMGVVGVIVPWNYPMIMAAWKIAPALAAGNSVVLKPSEKSPLTALRLAELAIEAGI
                     PSGVFNVVPGYGPEAGSPLALHMDVDCIGFTGSTRVGKQIHVMAGQSNLKRAWTELGG
                     KSPNIVFADCPDLDKAVEAAVGSIFFNQGESCNAPSRLFVEASILEQFLEKAVALAPK
                     FAPTDPLDPATIMGAIVDKTQMETVLRYVESGKAEGASLLYGGEQALAQSGGFYVQPT
                     IFAGVTSSMAICREEIFGPVLSVLSFTDATEVVRLANASVYGLQAAVWTRDINKAHGV
                     ARALRAGTVHVNQYDEDDITVPFGGYRQSGVGRDKSLHAFDKYTETKTTWIRIDSPV"
     gene            1866..>2187
                     /locus_tag="PLB25_20920"
     CDS             1866..>2187
                     /locus_tag="PLB25_20920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010100718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HOE44069.1"
                     /translation="MSQQPAQPLSREQQIAALEKDWATNPRWKGIKRDYSAADVVRLR
                     GSLPIEHTLARRGAEKLWDLVNGGSKKGYVNAFGAISAGQAMQQAKAGLEAVYLSGWQ
                     VAADG"
BASE COUNT          474 a          596 c          665 g          452 t
ORIGIN      
        1 ccgctgcact gaggccgaca ttgattcagc cgtcagtgcc gcccgggctg cctttgacga
       61 caagcgctgg tgtggcatgt caccggcggc tcgcaagcgg gtgatgatca agtttgccga
      121 ccaattactc gtccacgcag acgagctggc gctgatggaa acgctggaca tgggcaaacc
      181 gatcaagtat tcacgtgcgg tggatgtcaa cagcgcagcc aactgcatcc gctggtacgg
      241 tgaggcagtg gacaagatct acgacgagat agcccccacc gccagcactg ccctggcgct
      301 gatcacccgc gagccgatgg gtgtggtggg ggtgatcgtg ccgtggaact atccgatgat
      361 catggctgcc tggaaaatcg cgccggcgct ggccgcaggc aactcggtgg tcctcaaacc
      421 cagcgaaaag tcgccgctga cggcgttgcg tctggctgag ctggcgattg aggccggtat
      481 cccgagcggt gtgttcaacg tggtgcccgg ctacggcccc gaggccggtt caccgctggc
      541 gctgcacatg gatgtggact gcatcggttt taccggctcc acccgcgtgg gcaagcaaat
      601 tcatgtgatg gccgggcaga gtaacctcaa gcgggcctgg accgaactcg gcggcaagtc
      661 gcccaatatc gtctttgccg actgcccgga cctggacaag gcggtggaag cggcggtggg
      721 cagcatcttc ttcaaccagg gcgaaagctg caacgcacct tcacgcctgt ttgttgaggc
      781 cagcatcttg gagcaatttc tggaaaaggc ggttgccctg gcacccaaat ttgccccgac
      841 tgacccgctg gacccggcca ccatcatggg tgcaattgtt gacaagaccc agatggagac
      901 ggtgctgcgt tatgttgaga gtggcaaggc ggaaggggcc agcttgttgt acggcggcga
      961 gcaggccctg gcgcaatctg gcggcttcta tgtgcaaccg acgatattcg ccggggtgac
     1021 ttcgagcatg gccatttgcc gggaggagat ttttggtccg gtgctgtcgg tcctgtcttt
     1081 taccgatgcc accgaggtag tgcgcctggc caatgccagc gtttacggct tgcaggcggc
     1141 ggtgtggacg cgcgacatca acaaggcgca cggcgtggcg cgggcgctgc gggccggcac
     1201 ggtgcatgtg aaccagtatg acgaggacga catcacggtg ccgtttgggg gctaccggca
     1261 aagcggggta gggcgcgaca agtcgctgca cgcctttgat aaatataccg aaaccaagac
     1321 cacctggata cgcatcgaca gccctgtcta gatgattcat cgactcttgg ccgatgccaa
     1381 acctttcaat aaagtcgagt tgcggtttca tgtcggcgag cctgatgcct tcaatacaca
     1441 ttggccccaa cgcaggactc ctggcaccgt gcttggcgcg tgggtgcggc gagcggtggc
     1501 tagcaggcgc tgagtcagtg ctggctattg aagccaaagg agccgccgcc ataccttggg
     1561 cagacctggc gcaggcgcta tgcgtgcctg ccgccagtgg actttgaatt tttcaataac
     1621 agtgatctca tttcacaatt tgaaatttgg ttcgacgggt gggataaaaa atttcttaag
     1681 ctgaaaaaac tgttttcata ttgcgaaaat ggcaatttaa gctattgatt tatatcaaaa
     1741 taataaaagt cttctataag acataagaga caattctagt cttatataag actagaatac
     1801 cgaggatgac ttcagcaaac ctttcatgct gatgcgcctg ttttatcaac cctaggagtc
     1861 cattcatgtc acaacaacct gcccaaccac tgagccgtga acaacaaatc gccgcgcttg
     1921 aaaaagactg ggctaccaat ccccgctgga aaggaatcaa acgtgactac agcgccgcag
     1981 atgtggtgcg tttgcgtggc agtctgccca tcgagcacac gctggcccgc cgtggtgctg
     2041 aaaagctgtg ggatctggtc aatggtggct ccaagaaagg ctacgtcaac gctttcggtg
     2101 ccatcagtgc cggccaggcc atgcagcaag ccaaggctgg cctcgaggcg gtctatctgt
     2161 ctggctggca agttgcggct gacggca
//
LOCUS       DAOSLL010000687         2183 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_43314_length_2183_cov_4.813440, whole genome shotgun sequence.
ACCESSION   DAOSLL010000687 DAOSLL010000000
VERSION     DAOSLL010000687.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43314_length_2183_cov_4.813440"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            315..566
                     /locus_tag="PLB25_20925"
                     /pseudo
     CDS             315..566
                     /locus_tag="PLB25_20925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011408623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(767..1396)
                     /locus_tag="PLB25_20930"
     CDS             complement(767..1396)
                     /locus_tag="PLB25_20930"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HOE44070.1"
                     /translation="MQRRNILAGIASLALLGACGKAAKLSAIAPGQVVLAFGDSVTFG
                     TGAAPGEDWPSQLAEQTGWQVINAGIPGDTAQAGKARIQALLDEHHPALVIVEIGGND
                     FLRRRPPNDVKGDIRTLLKSIKQSGAQVVLVAIPELSLMSIVAGKPSDSPIFKELGKE
                     EGVLIVSDVFSDVLSNPELCADKVHPNAKGYRQMAGGLVARFREIGLIR"
     gene            complement(1640..>2183)
                     /locus_tag="PLB25_20935"
     CDS             complement(1640..>2183)
                     /locus_tag="PLB25_20935"
                     /inference="COORDINATES: protein motif:HMM:NF016705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HOE44071.1"
                     /translation="IVHGDMDAASGKGFRAIADYIFGNNVTTGSSPDKGSEQIAMTAP
                     VTMEPVNTASQKIAMTAPVTMEPLPDVGQASQTQQAMQGAKGWRVHFVMPSSYSLNTL
                     PKPNNRAVHLREVPAKTWAVLVYSGLNTEAGVQQRTNELVAWLAEQKIRTTGSPQLAR
                     YNPPWTLPMFRRNEIMLEIE"
BASE COUNT          498 a          587 c          560 g          538 t
ORIGIN      
        1 gccttcacgg taggactcgc gcagcaaaac ggtgggggcg gagccacgat ttggggcgat
       61 atttatttgc atgtctacat atttgcactc aaaacaaaac tacgtgttga tttcaaaagg
      121 aattcaacca aatccggatg gaacttcagc ttaatgcgtc ggccttaatt tcactcaaca
      181 ggttctggac agagcagtgg cacgagacgt tggatttcca gcgctggtgc ggtttgcggg
      241 tggtggatgg cgattgctgt ggccttcgtg tgccgacctg gaacgagacc atcatcgctt
      301 agtgcaccaa gtgtgtgatg cgctccaagg ttgaaccaat gaaagtggtc gccaagatga
      361 ttcgatctga tttggagggt atcgcggcgt ggacacagac acgccaaacc aatggattcc
      421 tggaagctat caacggcttg ttccaagcat ccaaacgccg tgctcgtggc ttcacacctc
      481 tctccactat caggacggtt atcttcttga tcgccggaaa actcgacttc caagccgtca
      541 accctcacgc ttggctaccc agttgaaatt cattggagtc ttatttttgc tcggccagcc
      601 aagccaccaa cgcattggtg cgctgctgaa ctgcggcatc agtgctcgta cctgaatgga
      661 ccaataccgc ccaggtttgg gcaagcactt cacgtaactg cacagccgtc ataggtgcca
      721 gcggtcacga tttggcatcc aaatgaccac ctattggtgt gcaccactag cgaatcaaac
      781 cgatttctct gaaccgtgcg accaagccgc ccgccatctg gcggtatccc ttcgcattcg
      841 gatggacttt gtcggcacag agttcagggt ttgaaagaac gtcagaaaac acatcactca
      901 cgatcagtac cccctcctcc tttcccaatt ccttgaagat cggtgaatcg ctcggtttgc
      961 ctgcgacgat gctcatgagc gacaactccg gtatcgctac aagcacaacc tgtgcaccag
     1021 attgtttaat cgacttgagc aaagttcgta tatcgccttt gacatcattg ggtgggcgcc
     1081 tgcgcaaaaa atcattgcca ccgatctcga caatgaccag ggccgggtga tgctcgtcaa
     1141 gcaatgcctg aattcgggcc ttacccgctt gtgctgtatc tcccggaatg cctgcattga
     1201 taacctgcca tccagtttgc tctgccagtt ggctgggcca atcttcacct ggtgcggcac
     1261 cagtgccaaa agtgacgctg tcaccaaaag cgagaactac ctgacctggc gcaatcgcag
     1321 aaagctttgc cgcctttcca caagcaccca gtagtgccaa actggcgata ccagcgagta
     1381 tgtttcgacg ctgcatgaga attcatctac ctgttttgac gttagccttc aaaattcaga
     1441 ttcaattgtc acaatgtttt gcctgtcttc aggtcgccaa tggctacctt ctattgctac
     1501 gaaacgagat cggtgttggc aacacaccgg tcgggtgtca agtctggtca ggttcgtgtc
     1561 agccggtgca gcagagaaag ctgacgcaca gcagtatgcg tcagcatgag agtaaccgtg
     1621 agggtgaaca gccgcttggt tactcaatct ccagcatgat ttcattgcgg cgaaacattg
     1681 gcaaggtcca gggtgggttg tatcgtgcca gttgtgggct gccggtggtc cttatttttt
     1741 gctcagccaa ccaagccacc aattcattgg tgcgctgctg aactccggct tcggtgttca
     1801 aacctgagta aaccaatacc gcccaggttt tggcaggcac ttcacgcaag tgcacagccc
     1861 ggttgttggg tttaggcaat gtgttcaggc tgtaagaact gggcatcaca aaatgcaccc
     1921 gccagccctt ggcaccttgc atggcttgct gagtctggct ggcttgtccg acatctggca
     1981 agggttccat ggtcacaggc gcagtcatgg cgattttttg cgaagcagtg ttgacgggtt
     2041 ccatcgtcac cggtgcggtc atcgcaattt gttctgagcc cttgtctggt gatgaacccg
     2101 tagtcacgtt gttgccaaat atgtaatctg caatggcgcg aaaacctttg cctgaagcag
     2161 catccatatc gccgtgcaca atg
//
LOCUS       DAOSLL010000688         2179 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_43428_length_2179_cov_4.118644, whole genome shotgun sequence.
ACCESSION   DAOSLL010000688 DAOSLL010000000
VERSION     DAOSLL010000688.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43428_length_2179_cov_4.118644"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="PLB25_20940"
     CDS             <1..642
                     /locus_tag="PLB25_20940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020227442.1"
                     /note="part of four member fumarate reductase enzyme
                     complex FrdABCD which catalyzes the reduction of fumarate
                     to succinate during anaerobic respiration; FrdAB are the
                     catalytic subcomplex consisting of a flavoprotein subunit
                     and an iron-sulfur subunit, respectively; FrdCD are the
                     membrane components which interact with quinone and are
                     involved in electron transfer; the catalytic subunits are
                     similar to succinate dehydrogenase SdhAB; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     flavoprotein subunit"
                     /protein_id="HOE44072.1"
                     /translation="VGECACVSVHGANRLGTNSLLDIVVFGRAAGKHIVQFNNENDKH
                     KPVPENGADVSLVRINRLDTSKSGEYAQKVGDDIRDTMQMHASVFRTQASMDQGVVKI
                     AAMREAVNNITLADKSRIFNTARIEALEVDNMMEAAQATMVSAAARKECRGAHTVLDY
                     DRPADDATCPLGRDDANWLKHTLWDRKTNSLSYKPVNLKPLTVASVPPKVRTF"
     gene            693..1397
                     /locus_tag="PLB25_20945"
     CDS             693..1397
                     /locus_tag="PLB25_20945"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019427020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HOE44073.1"
                     /translation="MAKRTFQIYRYNPDTDAKPYMQTVELELDGSERMLLDVLGKLKK
                     LDPTLSYRRSCREGICGSDAMNINGKNGLACLINMRTLPDTIVLKPLPGLPVIRDLIV
                     DMTLFFQQYHSIKPYLVNDTRPPEKERLQSPEERDELNGLYECILCASCSTSCPVFWW
                     NPDKFVGPAGLLQAYRFIADSRDEATSERLDNLEDPYRLFRCTTIMNCVDVCPKGLNP
                     TVAIGKIKELMVRRAI"
     gene            1408..1707
                     /locus_tag="PLB25_20950"
     CDS             1408..1707
                     /locus_tag="PLB25_20950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009518811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HOE44074.1"
                     /translation="MPNAASVPPGELVGQRALSKLRWRCRRGLLENDIFIARFFEKFE
                     SSLTVKQAQGLTALMDLSDNDLLDLHLGRKTLAQVQPDLARDDVSEVLSMLRAPP"
     gene            1717..>2179
                     /locus_tag="PLB25_20955"
     CDS             1717..>2179
                     /locus_tag="PLB25_20955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013595339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HOE44075.1"
                     /translation="MKLVENKATLSFSNGSPSVELPVYGGSIGPDVIDIRKLYAQTGM
                     FTYDPGFLSTASCQSAITYIDGDKGELLYRGYPIEQLATQCDYLDTCYLLLKGELPNQ
                     KAREDFHKLVIHHTMVNEQMQFFLRGFRRDAHPMAVLTGLVGAMSAFYHDST"
BASE COUNT          518 a          615 c          570 g          476 t
ORIGIN      
        1 gtgggcgaat gtgcctgtgt cagtgtccat ggtgccaacc gtcttggcac aaactcgctg
       61 ctggatattg tggtgtttgg ccgtgccgca ggcaagcaca ttgtccagtt caacaacgaa
      121 aacgacaaac ataagcccgt acccgaaaat ggcgcagatg tttccttggt gcgcatcaac
      181 cggctggata cctccaagtc tggagagtac gcgcaaaagg tgggtgacga catacgcgac
      241 acgatgcaaa tgcacgccag cgtgtttcgc acacaagcaa gcatggacca aggtgtggtc
      301 aagatcgcag caatgcgtga agcggtcaac aacatcacgc tggctgacaa gtcaaggatc
      361 ttcaatacgg cgcgtatcga agcgctggaa gtggacaaca tgatggaagc cgctcaagcc
      421 accatggtat cggctgccgc ccgcaaggag tgccgtggtg cccataccgt gttggactac
      481 gaccgcccag ccgacgatgc cacttgccca ctgggccgcg acgacgcaaa ctggctcaaa
      541 cacacgctgt gggatcgcaa gacaaacagc cttagttaca agccggtcaa cctcaaaccc
      601 ctgacggtag cctccgtgcc acccaaggtc cgcactttct gatcgtgtcc gtacagcagt
      661 ttcctgcacc caaccgaaca agagaaccca tcatggcaaa acgcacattt cagatttacc
      721 gctacaaccc agataccgat gccaagcctt atatgcagac cgttgagctc gaactcgacg
      781 gcagcgagcg tatgctgctc gacgtgcttg gcaaattgaa gaaactcgac cctaccctgt
      841 cgtaccgccg ctcgtgccgc gaaggcatct gtgggtcaga tgcgatgaac atcaatggca
      901 agaacggtct ggcctgcctg atcaatatgc gcactttgcc cgacaccatt gtgctcaaac
      961 cactgcccgg tctgccagtg attcgtgacc tgatcgtgga catgacgctg ttcttccagc
     1021 agtaccattc aatcaaaccc tacctggtca acgacacccg tccacctgaa aaggagcgct
     1081 tgcagagccc cgaagagcgc gacgagctca atggcttgta cgagtgcatt ttgtgtgcca
     1141 gttgctccac cagttgtccg gtgttctggt ggaatcctga caagtttgtt ggcccagcgg
     1201 ggctgctgca agcctaccgt ttcattgctg acagtcggga tgaagccaca agtgagcgcc
     1261 tggacaacct tgaggacccc tatcggcttt tccgctgcac taccatcatg aactgcgtag
     1321 atgtgtgccc caagggtttg aatccgacag ttgctatcgg caagatcaaa gagttaatgg
     1381 tgcgtcgcgc catctgaagc gctgaccatg cctaacgctg cgtcagtacc cccaggcgag
     1441 ctcgtcgggc aaagagcgct cagcaaattg cgctggcggt gtcgtcgcgg ccttctggag
     1501 aacgacatct tcattgcgcg ttttttcgag aagttcgagt cttcgctgac cgtaaagcag
     1561 gcgcaaggtc taaccgctct aatggacctg agtgataacg atctgcttga cctgcatctg
     1621 ggccgcaaaa ccttggcgca agttcagccc gatctggcgc gcgacgatgt atctgaagtt
     1681 ttgagtatgt tgagagcacc cccctgaaag atcgatatga aattagttga aaataaagcc
     1741 accctgtcgt ttagcaacgg cagtcccagt gttgaattgc cggtttatgg cggtagcatt
     1801 ggccctgacg tgatcgatat tcgcaagcta tatgcgcaaa ccggaatgtt cacttacgac
     1861 cccggtttcc tttccacagc ttcatgccaa tccgcgatca cttatatcga tggcgacaag
     1921 ggtgaattgc tgtatcgggg ctatcccatt gagcaattgg ccacccaatg tgattacctc
     1981 gacacctgtt atctactgct caagggcgag ctccccaatc agaaggcacg agaagatttc
     2041 cataagcttg tgatccacca cacgatggtc aacgaacaaa tgcagttttt cctgcggggt
     2101 tttcgtcgtg acgcacatcc tatggccgta ttgaccggtt tggttggggc catgtcggcg
     2161 ttctaccatg acagcactg
//
LOCUS       DAOSLL010000689         2175 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_43530_length_2175_cov_5.174528, whole genome shotgun sequence.
ACCESSION   DAOSLL010000689 DAOSLL010000000
VERSION     DAOSLL010000689.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43530_length_2175_cov_5.174528"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            380..823
                     /locus_tag="PLB25_20960"
     CDS             380..823
                     /locus_tag="PLB25_20960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44076.1"
                     /translation="MIDKGKPSAMPGLKKFDISGSLDLAVGQLESLCKAPYLQRLLFV
                     DISILRHAELAAFENCRAMGMSAAMSGSQFERHMRCRWMVTLGSERVLNWRVEQPPFM
                     TNIATDSILRLKFKQNQIGQHRHQSPQVANYNCRHRLGGKLSWLT"
     gene            1927..>2175
                     /locus_tag="PLB25_20965"
     CDS             1927..>2175
                     /locus_tag="PLB25_20965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44077.1"
                     /translation="MSHFRKIVLYGGLACVGLMLSGCVVAPRPYRPYPAHSAAVQPPV
                     AIESEYVQTAPPSPYTEVVPSSPGVGYVWVPGLWLWSAG"
BASE COUNT          488 a          534 c          637 g          516 t
ORIGIN      
        1 ttgccgaagg ggtcgagttg caggcgcaag ccgaatttct ggcgcaccag ggctgccatg
       61 cttaccaggg ctatttgttc agccgaccgc tgccgctggc ggcgctggag gcgtttgcgc
      121 gccggcctgg ctgaagcagt ctgcgtgctt catgacggca ccacctgcgg ttgccggcct
      181 ggtgtcagat attcaagtaa ataatgcctg aagcgcccgt gttgattgcg ctagcagcta
      241 tgaaaatgat atctaatcgg caatcaaccg agccgcgtta cggtcggcct gggtgatgcc
      301 gatgcccgat acacagcgcg cttggcctgc atgggcaggt tcaaatgcaa agccaagcat
      361 gaattggcgg actggcgccg tgattgacaa aggtaaacct tcggctatgc caggactgaa
      421 aaagtttgat atttccggaa gtctggactt ggccgtaggc caacttgagt cattgtgtaa
      481 agcaccgtac ctgcaacggc tgttgtttgt tgacatatcg atcctgcgcc atgctgagtt
      541 agctgccttt gaaaactgca gggccatggg tatgtccgcc gccatgagcg ggtcacagtt
      601 tgaaaggcac atgcgttgcc ggtggatggt cactttgggg tcggaacgcg tcctgaactg
      661 gcgtgttgag caaccacctt ttatgaccaa cattgctacc gactcaatac ttcgactcaa
      721 atttaaacag aaccaaattg gtcaacatcg ccatcagtca ccacaagtag ccaattacaa
      781 ttgccgccat cggttgggcg gaaagctcag ttggttaacg tgaaataact ttcatgtccg
      841 ggggtgaatg gagtccatga aagttagagc aggggactga caatcaattg ctgtgttcgc
      901 ggacttgaaa ctccgtactg gcacggtcct agcgggctgg cgttgcaatt gtttgtttgg
      961 cagtggtttg ttgaagaaat tcggcaaatc tttttgacat gggtgcggtt gcaataatgc
     1021 taccgatgtg cgcgttgcaa ccgtaatgca gccagcaatg ggtggttgtc attgaacagc
     1081 agtgaacacc gatgctcaat gctggtaaag gttttgggcc gttagctcag ttggttagag
     1141 cagaggactc atcgagatgg tgaccctgct gcgaaagtgg cagtggaaaa accgggtgaa
     1201 ctcagggaat ccgtcagtca gtcaatgacc cggcaatcct gagccaagct cactgaaggc
     1261 agatcgcaag atcggtaagt gagaaggtgc agagactagg atgtgaggag ccaatccgat
     1321 aagcatccca cgagcgcccg gcatcctaac gaacggcgca atccgtggcg aggatgatga
     1381 gatagtccag ggaatgggga aactcatgca aacctgaatc ctttggtcgt aggttcaagt
     1441 cctacacggc ctaccagaac ttccagaagc cacccaacgc ggtggcttct gtttttgtgg
     1501 cttccttcaa gcgactgccc tgggcgcaag gccccgctca tcgatggcaa aacaggctgc
     1561 caaaaggtac ggctccggca aaagcaggcc aaacctggaa cgtgattgcc aacgcaagaa
     1621 aaaccggccg gtcagggctt taaattcaga ataaacccga tatgcttgca cgataggcaa
     1681 ctggcgaagc agttttggcg gggtattcgg tcaggcactg agtattttca cttgttgctg
     1741 gcactaatgc ccggttaact gagtcagacc tgtcggggct tgcattgttg tggaatgtcc
     1801 actttttagc gtgatggggt tgagccgttg ccaccttggc cgcaagcccg ccattgttac
     1861 cctcacgctt gatcaacagg gttttcgggt ttagagtcaa tgtcacttag tttcaggaga
     1921 aagaatatgt cccatttccg gaaaattgtt ttgtatggcg gtttggcatg tgttgggctg
     1981 atgctgtcgg gttgcgtcgt tgcacctcgc ccctatcggc cttatccggc tcactcggca
     2041 gcggtgcagc cgccggtggc tatcgagagc gagtatgtcc agaccgcgcc gccttcgcct
     2101 tacaccgagg tcgtgccatc ttcgccgggt gtcggctatg tatgggttcc agggctttgg
     2161 ctgtggtcgg ctggt
//
LOCUS       DAOSLL010000690         2157 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_44031_length_2157_cov_4.112750, whole genome shotgun sequence.
ACCESSION   DAOSLL010000690 DAOSLL010000000
VERSION     DAOSLL010000690.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44031_length_2157_cov_4.112750"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="PLB25_20970"
     CDS             complement(<1..216)
                     /locus_tag="PLB25_20970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019429961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 family protein"
                     /protein_id="HOE44078.1"
                     /translation="MTPSTALIGFGQVRHARLKPRHHAFAYPTYFLLLPMRSLQANGP
                     GALARNRRALLSFYDTDHGDGRSIANGG"
     gene            complement(299..1585)
                     /locus_tag="PLB25_20975"
     CDS             complement(299..1585)
                     /locus_tag="PLB25_20975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE44079.1"
                     /translation="MKIAIIGSGISGLAVAHQLRGRADITLFEAGSYFGGHTHTVDIT
                     LPTAQGPVTHGVDTGFLVFNERTYPNLIRLLAELDVATARSDMSFSVQAPTDKPGLTL
                     QWSGASLNAVFAQRRNLVNPRFWRMLRDLLRFNRLATELAISGKAAALLQPLADFLTQ
                     QRFSAEFRDWYLLPMLGCIWSCPVDQMLQFPVATMIRFCHNHGLLQVSDRPQWWTVQG
                     GARHYVDKITAQIADKRLNSPVQQIERDAQGVTIHAAGQSGRFDKVVLACHPDQALTL
                     LADASVQERKTLGAIRYQANRAVLHIDSSVLPQSRRAWAAWNYQRVASPGAAPAEARV
                     CLHYLINRLQPLPFDQPVLVSLNPLSAIDPGSVLGVFDYAHPVFDQAAIEAQQQVPAL
                     QGQQHSYFCGAWTGYGFHEDGLKSGLAVAHLLAPAV"
     gene            complement(1982..>2157)
                     /locus_tag="PLB25_20980"
     CDS             complement(1982..>2157)
                     /locus_tag="PLB25_20980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010457952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin molybdenumtransferase MoeA"
                     /protein_id="HOE44080.1"
                     /translation="NTSSSADGTLQVKTTGDQGSGVLSSMVRANGLIVLHHRQGNVAA
                     GDAVDVMMFDGVV"
BASE COUNT          434 a          636 c          665 g          422 t
ORIGIN      
        1 gccgccattg gcgatgctgc ggccgtcgcc atggtcggtg tcatagaaac tcaacagtgc
       61 ccggcggttg cgcgccagcg cgcccgggcc attggcttgc aggctgcgca tcggcagcag
      121 caaaaaatag gtcgggtagg caaaggcgtg gtgccggggt ttgagccgcg catggcgcac
      181 ctggccaaag ccaatgagcg cggtggaagg tgtcatgatc atctttcgtc gttccgaaat
      241 ttatcaaaaa tgatagctgt cagcgcttta ttcataaggg ctagagcctg atttatcatc
      301 aaaccgcagg ggccaacagg tgcgctaccg caaggccgga cttcaggcca tcctcatgaa
      361 agccatagcc agtccaggca ccgcagaaat agctatgttg ctgcccttgc agcgctggca
      421 cctgttgttg cgcttcgatg gcagcttggt caaacaccgg atgcgcatag tcaaaaacac
      481 ccagcaccga gcccgggtca atggcactca acgggttgag cgacaccagc accggctggt
      541 caaatggcag cggctgcagt cggttgatca ggtaatgcag gcaaacccga gcctcggcgg
      601 gagccgcgcc agggcttgcc acgcgctggt agttccaggc ggcccaggca cggcgtgact
      661 gcggcagcac cgaactatcg atgtgcagca ccgcacggtt ggcctggtac ctgatggcac
      721 ccagcgtctt gcgctcctgc acggatgcgt cggccagcag cgtcaaagcc tgatccgggt
      781 ggcaggccag caccactttg tcaaaacgcc cgctttgtcc ggcggcatga atcgtcacgc
      841 cctgggcatc acgctcgatt tgttgcaccg gcgagttcag ccgcttgtcg gcgatctggg
      901 cggtgatttt gtccacgtag tggcgtgcgc cgccttgcac ggtccaccac tgcggtcggt
      961 cgctgacctg cagcagcccg tggttgtgac agaagcggat catcgtcgct accggaaact
     1021 gcagcatctg gtccaccggg cagctccaga tgcaacccag catgggcagc agataccagt
     1081 cgcgaaactc tgccgagaac cgctgctgcg tcaaaaagtc agccaatggc tgcagcagcg
     1141 ccgcagcctt cccgctgatg gcaagttcgg ttgccagtct gttaaagcgc agcaggtcgc
     1201 gcagcatgcg ccaaaagcgc gggttgacca ggttgcggcg ctgggcaaac acggcgttga
     1261 gcgaagcacc gctccattgc agcgtgaggc ccggcttgtc agtgggcgct tgcactgaaa
     1321 acgacatgtc tgatctggca gtggccacat caagctcggc cagcagccgg atcaggttgg
     1381 gataggtgcg ctcgttgaac accagaaagc cggtgtccac gccatgtgtg accgggcctt
     1441 gcgctgttgg cagggtgata tcgacggtgt gcgtgtggcc accaaagtag ctgcctgcct
     1501 caaacagggt gatatcggcg cggccccgga gctgatgcgc cacggccaga ccagagatgc
     1561 cagaaccaat gatggcgatt ttcatgaaag tgccttgtgc agcttgtaac ggtgttaaaa
     1621 cgagtatgga ttttttgtga ctgattggta atttatcaca tcaatcagtt ttgtgcattg
     1681 ccaatgggga tttcggcgac gacagaccgg ttggtgcaca ggccgacagc ctgtcaggtt
     1741 gaaggcggtt tggccgagcc gccggacttc attgagtcgg ctgcgctgat ggtcgccacc
     1801 gccaagcgtt ggcgttcaat ttggttgagt gattgggtct tggcaccaac acttgccgct
     1861 gctaagggct tttgcagcac cacaagcccc gtcaggtgcc agcgcggcca catctgacac
     1921 agtggccgac atcattgcgc ggccgggttg cggcgcgtca gttcacgcaa ggcatgccgc
     1981 ctcagaccac cccgtcaaac atcatcacat caactgcatc acctgccgcc acattgccct
     2041 gccggtggtg cagcacgatc aaaccgttgg cgcgcaccat cgaactgagc acgccagagc
     2101 cctgatcgcc agtggttttg acttgcaggg tgccatcggc gctgctgctc gtgttcg
//
LOCUS       DAOSLL010000691         2142 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_44436_length_2142_cov_5.317681, whole genome shotgun sequence.
ACCESSION   DAOSLL010000691 DAOSLL010000000
VERSION     DAOSLL010000691.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44436_length_2142_cov_5.317681"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="PLB25_20985"
     CDS             <1..872
                     /locus_tag="PLB25_20985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HOE44081.1"
                     /translation="HDGAAGAPLALLIDGHVQMFDCIEFNPELRWIDVASEVAFTYVD
                     LLVHQQAGLANWFLNLWLSHNGDYAAAPLIRFYAVYRALVSAKVAAIRAGQSHGDDDQ
                     ALKHLAQAEQLTITLPLQLTITHGLSGCGKTVAAQAMLQSACTNPTLCLRSDVERKRQ
                     FGLGATAQSGSALDDGIYTVDAHARTYARLLELATGLLRCGWSVIVDAAFLQAADRDR
                     FRALAHSAGASFSILAPVATADQLRERILARQTLGHDASEATLEVLARQLKTVQPLAT
                     HEPIWPTIDLPTS"
     gene            1058..>2142
                     /locus_tag="PLB25_20990"
     CDS             1058..>2142
                     /locus_tag="PLB25_20990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HOE44082.1"
                     /translation="MTSKTSAPVEPHSATGLPNTSGELANSTFPLLTNNLPSFPIVGI
                     GASAGGLAAFEAFFSGLPKDVETGMAFVLVQHLAPDHKSMLPELIRRYTPMPVFEVED
                     SMAVAPNCVYIISPNHDMALLNGSLQLLEPTAPRGQRLPIDFFFRSLAADQHERAIGI
                     VLSGTGSDGTLGVRAIKSAGGMVMVQAPENAEFDGMPRSAIATTVVDYQINADQMVAQ
                     LINYLKYASDNFSIRAGKPSPISESSLKKIFVLLRTKTGHDFSQYKPSTVHRRIERRM
                     SVQQISLIDEYVRFLQHAPEEIEALFRDLLIGVTSFFRDPEAFQALEEQVIPLMFAGK
                     STSNGVVRIWVTGCSTGEEAYSLAILL"
BASE COUNT          418 a          668 c          619 g          437 t
ORIGIN      
        1 cccatgatgg cgcagccggc gcacccctgg ctctgctgat cgacggccat gtgcagatgt
       61 ttgactgcat tgagttcaac cccgaattgc gctggattga cgtggccagc gaggtcgcgt
      121 tcacctatgt tgacctgctt gttcaccagc aagccgggct tgccaactgg tttctgaacc
      181 tgtggctgag ccacaacggc gactacgcgg cagcgccgct gatcaggttc tatgcggtct
      241 accgcgccct ggtcagtgcc aaggtggcgg ccatacgcgc cgggcagtcc cacggcgatg
      301 acgaccaggc gctcaagcat ctggcgcagg ccgaacagtt gacgatcacg ctgccactgc
      361 agttgaccat cacgcacggt ttgtcgggtt gcggcaagac ggtggcggcc caagcaatgc
      421 tgcaaagtgc ctgcaccaac cccaccctgt gtctgcggtc ggacgttgaa cgcaaacgac
      481 agttcgggct gggggccacc gcccaaagcg gctcggcgct ggacgacggc atttacaccg
      541 ttgatgccca tgcgcgaacc tatgcgcgcc tgctcgaact tgccaccggc ttgctgcgct
      601 gcggctggtc ggtgattgtc gatgcggctt ttttgcaagc agccgatcgg gaccgctttc
      661 gcgcgctggc acacagcgcc ggggccagct tcagcattct tgccccggtt gccacggcag
      721 atcagttgcg cgagcgcatt ctggcgcgcc agacgcttgg gcatgatgcc tctgaagcaa
      781 ccctggaggt gctggcacgg cagttgaaaa cagtgcagcc cctggccacc cacgagccca
      841 tctggccgac catcgattta cccacaagtt aagcatcaaa ttgggctcta gcccttgtcc
      901 tgtatgcgct accagctatc tattttgatg ttatgtgcga atcctccagg catcctgact
      961 gtttcccaat ttacccaggc agcagcgcaa gccctacact cgcccgaggt gctcaaaggc
     1021 aaccgccaga caatagccag caccaacagg agcgcgcatg accagtaaaa catcggcacc
     1081 agtcgagccg cattcggcca ccggcttgcc caatacttcc ggggaacttg ccaacagcac
     1141 gttcccgctg ttgacgaaca acttgcctag ctttcccatc gtcggcatcg gggcatcggc
     1201 tggcgggctg gcggcttttg aggcgttttt ctcgggcctg cccaaagacg ttgaaactgg
     1261 catggccttt gtgctggtgc agcatctggc cccggaccac aaaagcatgc tgcccgagct
     1321 gattcgccgc tacaccccga tgccggtgtt tgaggtcgaa gatagcatgg cagtggcgcc
     1381 caactgcgtc tatatcatct cgcccaacca cgacatggcc ttgctcaacg gcagcttgca
     1441 gttgcttgag cccaccgcac cgcgtggcca gcgcttgccg attgacttct tcttcaggtc
     1501 gctggccgca gaccagcatg aacgggccat tggcatcgtg ctgtcgggca ccggcagcga
     1561 cggcaccctg ggcgtgcgag ccatcaagtc ggctggcggc atggtgatgg tccaggcccc
     1621 cgagaacgca gaatttgacg gcatgccgcg cagcgccatt gcgaccaccg tggtggacta
     1681 ccagatcaac gccgaccaga tggtcgcgca gctcatcaac tatttgaaat acgcctctga
     1741 caatttttcc attcgcgcag gcaagccctc gccaatcagc gaaagctcgc tgaagaagat
     1801 ctttgtgctg ctgcgcacca agaccgggca tgatttttcg cagtacaagc ccagcaccgt
     1861 ccaccgtcgc atcgagcgac gcatgtcagt gcagcaaatc agcctgattg atgagtatgt
     1921 gcgctttttg cagcatgccc cggaagagat cgaggcgctg tttcgtgact tgctgattgg
     1981 ggtgaccagc ttttttcgcg acccggaagc ttttcaggcg ctggaagaac aggtgatacc
     2041 gttgatgttt gctggcaagt ccaccagcaa cggcgtagtg cgtatctggg tgacgggctg
     2101 ctccaccggt gaagaagcct attcgctggc aattttgctg ca
//
LOCUS       DAOSLL010000692         2140 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_44494_length_2140_cov_3.965947, whole genome shotgun sequence.
ACCESSION   DAOSLL010000692 DAOSLL010000000
VERSION     DAOSLL010000692.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44494_length_2140_cov_3.965947"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(141..614)
                     /locus_tag="PLB25_20995"
     CDS             complement(141..614)
                     /locus_tag="PLB25_20995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44083.1"
                     /translation="MNLLTRWSKSHKLATFVTPSLGEDGKIACVIDPLAMQNNALLDG
                     CYCLETDLTREQMQAVTVHERYGDLQKVERNFRTMKTGLLEVRPIFLRNAERTKAHVF
                     VAMLSLKIARAFETMLHKTFGTVANEADALTIDDALVALSRLTYLHYETNGQTIT"
     gene            complement(820..1335)
                     /locus_tag="PLB25_21000"
     CDS             complement(820..1335)
                     /locus_tag="PLB25_21000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44084.1"
                     /translation="MNETKLRTIAQLQDFLKATPEVSFSGTGEKGDSERYDHISRVLK
                     RFDYPHRKKHDRGVVMAYLQHTSGYSRAQIKRLVARRHENRLADTPLVKRYCASAAPF
                     ARNYTASDIDLLVEMDRANEDVCGAAIVHLFKRAYNTYGDQGYERLATLPVLNRPTCP
                     EAVATKPCVSH"
     gene            1419..2120
                     /locus_tag="PLB25_21005"
     CDS             1419..2120
                     /locus_tag="PLB25_21005"
                     /inference="COORDINATES: protein motif:HMM:NF019225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HOE44085.1"
                     /translation="MNRSHIAALIISALATAHGAQAALINVAAGKTYAYYGNAPGEGE
                     YYHDDGHIVHTSGLGVFSMGELTDGILQAGSPDAQVLIPSPDVSFYGTMGRIVIDLGA
                     LYALSSVDLGTNRYQAFANFRPEAVNLLFSTDGSNFGVPVQWDVPTNASEGQMTFSFD
                     LPSTAIARYVQFDFATPGQPTTEIHFDELRAIGEAVTTTQHVPEPSSVLLASLALAGL
                     VCQRQMRRTSKDKCR"
BASE COUNT          455 a          588 c          600 g          497 t
ORIGIN      
        1 gccataaccc tttgaattta aatagttatt cttgtttgag cagaaaaatc ttactgccta
       61 caaggtgtgg gtgcactaac agggaaagtc aagccaagtg cgttgagtat cgaagactga
      121 accgcatctg gtcgaggcaa tcatgtgatc gtttgtccgt ttgtttcgta atgaaggtag
      181 gtcaggcgtg acagtgccac cagtgcatcg tcgattgtca gtgcgtccgc ttcgttggcg
      241 acagtgccaa atgtcttatg cagcatggtc tcaaaggcac gggcgatttt cagggagagc
      301 attgcgacga acacatgtgc tttggttcgc tcggcattgc gcaagaagat agggcgcact
      361 tcgagcaagc cggttttcat ggtgcggaaa ttgcgctcga ctttctgcag atcaccgtag
      421 cgttcatgaa ccgtaacggc ttgcatctgc tcgcgggtga ggtcagtctc caggcaataa
      481 cacccatcaa gcagcgcatt gttttgcata gccaatggat caatgacaca ggctattttt
      541 ccatcttcac caagggaagg tgtcacaaat gtggcgagtt tgtggctctt gctccagcgc
      601 gtgaggagat tcaatccggc ctgcgctttg gcccgaggac acttaccgac gaaggtacct
      661 gccactgact caccgcatcc acacaagtga tgtgatagac acccttgatg ctgtcaagat
      721 cgccctgatg cgcgctgtca atgcgcaccc accctgcacg cccattgggc cgtgcagctt
      781 tgcgcgcacc gactgggttg cacactgggt gagtcttggt cagtgtgaca cgcaaggctt
      841 ggtagccact gcttctgggc aggttggacg gttcaaaact ggcagggttg ccagacgttc
      901 ataaccctgg tcgccgtacg tgttgtaggc gcgtttgaaa aggtgcacga tggccgcacc
      961 acagacatct tcgttggccc gatccatctc cacgagcagg tcaatatcgc tggctgtgta
     1021 gttgcgggca aacggggctg ccgaagcgca gtagcgcttg accaacgggg tatcagcgag
     1081 ccgattctca tgccggcgtg ccaccagccg tttgatctgg gcgcggctgt agccactggt
     1141 gtgctgcagg taggccatta ccacaccacg gtcgtgtttt ttgcgatgcg ggtagtcaaa
     1201 gcgcttgagc acgcggctga tgtggtcgta tctttcgctg tcacctttct cgccagtgcc
     1261 gctgaagctg acctcgggcg ttgctttgag aaagtcttgc agttgcgcaa tcgtacgcag
     1321 ctttgtttcg ttcatgttga tcactcatga gtaaaatgct gggccgcaaa ttcccgtcct
     1381 taaaccgtct atccagcctt actgaaaggc gacacaccat gaatcgatct catattgccg
     1441 cgctgataat tagcgctttg gctaccgcac acggcgcgca agccgctctc attaacgtag
     1501 cagcgggcaa gacctacgcc tactacggca atgcgcccgg agagggcgag tattaccacg
     1561 atgacggaca catcgtccat acaagcgggt tgggtgtttt cagcatgggg gagctcaccg
     1621 acggcatcct ccaggcgggt tcgccggacg cccaagtgtt gatcccctcg ccggacgttt
     1681 ccttctacgg cacgatgggc cgcatcgtca tcgacctggg cgcgctttat gcgctaagct
     1741 cggtggacct gggtaccaac aggtatcaag ccttcgcaaa cttccgcccg gaagcagtga
     1801 atctattgtt cagcaccgac ggcagcaact ttggcgtgcc agtgcagtgg gatgtcccga
     1861 ccaatgcttc ggagggccag atgactttct cgttcgatct accttccaca gcaattgccc
     1921 ggtacgtgca attcgatttt gcgacaccag gacagcccac gactgaaatc cactttgatg
     1981 aacttcgtgc cattggcgaa gcagtgacaa cgactcaaca tgtgcctgaa ccttccagcg
     2041 tgctcttggc gagcctggcc ctggccggct tggtctgcca aaggcagatg cgcaggacct
     2101 caaaagacaa gtgtcgctga gcaagcgtct gaagcgcgcc
//
LOCUS       DAOSLL010000693         2129 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_44827_length_2129_cov_2.867406, whole genome shotgun sequence.
ACCESSION   DAOSLL010000693 DAOSLL010000000
VERSION     DAOSLL010000693.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44827_length_2129_cov_2.867406"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            208..537
                     /gene="raiA"
                     /locus_tag="PLB25_21010"
     CDS             208..537
                     /gene="raiA"
                     /locus_tag="PLB25_21010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HOE44086.1"
                     /translation="MNLTISGHHLEVTPALRSYVTTKLDRIKRHFDQVVDVKVLLTLE
                     NLKEKERRQKAECNIHIKGIELFAESAHADLYAAVDELVDKLDRQVVRHKDRLQSHHH
                     HAPKRLM"
     gene            621..1088
                     /locus_tag="PLB25_21015"
     CDS             621..1088
                     /locus_tag="PLB25_21015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012745567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HOE44087.1"
                     /translation="MNRLASILSAAQVLVQVDVSSKKRAFEEAGLLFENLHGLSRALV
                     TDSLFSRERLGSTGLGHGVAIPHGRIKGLKSPMAAVFQLARPIGFEAPDEQAVGLMIF
                     LLVPEAATQKHLEILSEIAEMLSDAGLREKLAASSSAADLHRLIADWQSVQSA"
     gene            1128..2078
                     /gene="hprK"
                     /locus_tag="PLB25_21020"
     CDS             1128..2078
                     /gene="hprK"
                     /locus_tag="PLB25_21020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HOE44088.1"
                     /translation="MKPTVISADVLFEAFRATLRWEWVAGLGASERRFDELAVRAARS
                     GADLVGYLNYIHPYRVQILGEREVAYLTNATAEDCSRRIARIVTLEPPVLILADSQVA
                     PDALLSMCERAQIPMFSTEGSSAFVIDVLRAYLSKHFADRCTMHGVFMDILGLGVMIT
                     GESGLGKSELGLELISRGNGLVADDAVDLYRINQDTIEGRCPELLLNLLEVRGIGLLD
                     IRCIFGETAVRRKKRLQLIVHLVRRETMERDYERIPYEPLTQDVLDVPVRKVVIQVVA
                     GRNIAVLVEAAVRNTILQIRGVDTYKEFVERHRKAMSEAS"
BASE COUNT          459 a          552 c          610 g          508 t
ORIGIN      
        1 taataatcct ttcagattat cgcattgaca gccactttaa accaaatgcc tgcacaccat
       61 acccagacaa tgttgcattg catcattaaa aattcgttgc attcaaacgt gatggcgtgc
      121 atagtgggtc caaggatgaa tcaagagttt tccggctgtc aacgaggtgt tggcagtctt
      181 ttccaccaaa agcttaggag gctttttatg aacttgacga tcagcggtca ccacctggag
      241 gttaccccgg ctttgcgctc ttatgtcacg accaaattgg atcggatcaa gcgccatttc
      301 gaccaggtgg ttgacgtaaa agttttgctg acactcgaga atctcaagga aaaggagcgc
      361 agacagaagg cggaatgcaa tattcacatc aaaggcattg aattatttgc tgaaagtgcc
      421 cacgcggacc tgtacgccgc agtggatgaa ttggtggaca aactcgacag gcaggtggtt
      481 cgccacaagg atcgcttgca gtcacatcat catcacgcgc ccaagcgcct gatgtaattt
      541 aaactctgcc aagccgcccg ctggtgcgtg cggctttttt ttgtgcccaa attttccggg
      601 tgcataattc aaagcaaatc atgaaccgac tcgcgtccat cctttctgct gcacaggtat
      661 tagtccaagt ggacgtttca agcaagaaga gagcttttga agaagctggc ttgctgtttg
      721 agaatctcca cggactgagt cgagcgctcg tcaccgacag cctgttctca cgtgagcggc
      781 tgggttccac cggtttgggg catggtgtgg ccataccgca tggtcgcatc aagggcttga
      841 agtcgcccat ggcggccgta tttcaactcg cccggccgat tggttttgaa gcgccagacg
      901 aacaagctgt cgggttgatg atttttctgc tggtgccaga agcagcaacg cagaagcatc
      961 ttgagatact ctcggaaatt gcagaaatgc tgagcgatgc cggtctgcgc gagaagcttg
     1021 cagccagcag cagtgccgcc gatctgcacc gcttgattgc agattggcaa tctgtccagt
     1081 ccgcttgacc caatcgcgcc aagttttatc tggctgaagg catctccgtg aaacctactg
     1141 tcatcagtgc cgacgttcta tttgaggcct ttcgagccac attgcgctgg gaatgggtgg
     1201 cgggtttggg tgcctcggag cgacggtttg acgagttggc ggtgcgcgcc gcccgctcgg
     1261 gggccgacct ggtgggctat ctcaactata ttcaccccta ccgtgtacaa attctgggtg
     1321 aacgcgaagt tgcctacctg accaacgcga cggcagaaga ttgctcgcga cgtattgcgc
     1381 gtatcgtcac tctggagcca cctgtgctca ttttggctga ctcgcaggtg gctccggacg
     1441 cactgctgtc aatgtgtgag cgggcacaga tccccatgtt ttccacagaa gggtcgtctg
     1501 cgttcgtgat tgacgtgctg cgcgcctacc tctccaagca ttttgccgac cgctgcacca
     1561 tgcacggtgt gttcatggac atcctgggtc tgggggtcat gatcacgggt gaatccggtc
     1621 tgggtaaaag tgaactgggc cttgaactga tttcccgtgg caatggtctg gtagccgacg
     1681 atgctgtcga tctctaccgc atcaaccagg acaccatcga gggccgctgc ccggaacttc
     1741 tgctcaattt gttggaggtg cgcggcatcg ggctgctgga cattcgctgc atttttggtg
     1801 aaacggcggt gcggcgaaag aaacgtcttc agctgattgt gcacctggta cgacgcgaga
     1861 ccatggagcg tgactacgaa cgtattccct atgagcccct gacgcaggat gtgcttgatg
     1921 taccggtgcg aaaggttgtg atccaggtgg ttgccgggcg caacattgcg gtgctggtgg
     1981 aggccgccgt gcgcaacacc attttgcaga ttcgcggcgt tgacacttac aaagagtttg
     2041 tcgagcgcca ccgcaaggcg atgagcgagg ccagttaagc ttcttcgggg cgatgctcac
     2101 agccgctgcg cgtgcagtgg gcgtaaagg
//
LOCUS       DAOSLL010000694         2124 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_44990_length_2124_cov_2.703238, whole genome shotgun sequence.
ACCESSION   DAOSLL010000694 DAOSLL010000000
VERSION     DAOSLL010000694.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44990_length_2124_cov_2.703238"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="PLB25_21025"
     CDS             complement(<1..995)
                     /locus_tag="PLB25_21025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE44089.1"
                     /translation="MRREIIALEALGVGVIRVAARPFRGELVDPTDVAEAKNTSYTTE
                     SLVRAATCLTRVALTRPLHLFKALGDVIRIGTQTKSGIWKNLMYLGQACVLLQLANKC
                     AHIHVNFGNATSIAIYCRILGGPPVSLRIHGPEEFIDSTPSDWAWKIRHAAFLAPITE
                     FGMKKVQSCVSPGRHPKIKLLRCGIEVALLTRGCDAAMRLPSELNLVCIARLESRKGH
                     DVLLKALHRLKQDGLTVKLTIIGDGSCRASLKKQARELSLSESIHFAGWQAGPEVILS
                     MRNARVVVLPSFSEGLPIVLMEALAIGRAVVTTRVDGIPELIFPGKTGWLVEAGDV"
     gene            complement(1202..1483)
                     /locus_tag="PLB25_21030"
     CDS             complement(1202..1483)
                     /locus_tag="PLB25_21030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HOE44090.1"
                     /translation="MSKRAPLIDADGEVRELTTADLRHFKPAAEVLPPDLLKVMGIKP
                     RGPQKAPTKQATTIRLSPDVMAAFKATGAGWQTRIDAALKDWLRTHSPV"
     gene            complement(1815..>2124)
                     /locus_tag="PLB25_21035"
     CDS             complement(1815..>2124)
                     /locus_tag="PLB25_21035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008328818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HOE44091.1"
                     /translation="KRPAHYLWAVLIARIYEVFPLLCPMCGGQMRLIAFITHSVDIRQ
                     ILEHIGVESEPPHIAPARGPPLWVECDAPVDDGVQGEPDWDLAAQPAPDYEVDQRVNW
                     "
BASE COUNT          443 a          574 c          604 g          503 t
ORIGIN      
        1 acgtctccag cctcgacgag ccaacctgtt ttgcctggaa aaatgagttc ggggatccca
       61 tcgacccgtg tcgtaaccac tgcccggccg attgccagtg cttccattag gacgattgga
      121 agcccctcag aaaaactcgg aagcacaact acgcgggcgt tgcgcattga cagaatgacc
      181 tctggcccgg cttgccagcc agcaaagtgt attgattcag aaagagacag ttctcgtgct
      241 tgttttttga gactggcgcg gcaggaacca tccccaatga ttgtgagttt gacggtcagc
      301 ccgtcctgct taagtcgatg cagtgctttc agtagtacgt catgcccttt cctggattcc
      361 aatcgggcaa tgcacaccag attaagttcg gagggtagtc gcatggccgc atcgcatccg
      421 cgcgttaata acgctacctc aattccgcat cgcaacaatt taattttggg atgtctaccc
      481 ggggaaacgc acgattgaac ctttttcatg ccgaattcgg taataggcgc aagaaatgcc
      541 gcatgacgaa tcttccatgc ccagtcggac ggagtggagt caatgaattc ttctggacca
      601 tgaatacgaa ggctgacagg tggcccgcca agaatcctgc agtaaatggc gatgctcgtt
      661 gcgttgccga aatttacgtg gatgtgtgcg catttatttg ccagttggag taatacgcac
      721 gcctgaccga ggtacatcag gtttttccaa atgccgcttt ttgtttgggt acctatgcga
      781 atcacgtcac cgagtgcttt gaatagatgc aatggacggg ttaaagcgac tcttgtgagg
      841 catgttgcgg cacgcaccag actttcagtc gtatagctgg tattttttgc ctctgccacg
      901 tcagtggggt cgacaagttc acctctgaat gggcgcgcgg cgaccctgat aacgcctacg
      961 cccaatgcct ctaacgcgat gatctccctg cgcatggtgg tgtggctggg agcggggtaa
     1021 acgtttgtga aataacaaac caacatttgt gcatctcgcg gtttttcgag tacggctgaa
     1081 gactgccata tttaagttgg cagcgccgac gtattaaatc gcaaccgcct ggtgtagcag
     1141 cagatgctgg ggccttaagt tttgggtggc ttgctgggtt ggcacgcggc gcaagtgacc
     1201 gtcaaaccgg cgaatgggta cgcagccagt ctttcaacgc cgcatcaata cgtgtctgcc
     1261 agcccgcgcc cgtggctttg aaagcggcca tcacgtcggg tgacaggcgg atcgtagtgg
     1321 cctgtttggt cggtgctttt tgcggcccgc gcggcttgat gcccatcact ttgagtaaat
     1381 caggcggcaa tacctcggct gctggcttga agtggcgcag gtctgcggtc gtaagttcac
     1441 ggacctcgcc gtctgcatca atcagtggtg cgcgtttgct catagtcttt ttgtcagtac
     1501 attgtcatgc agcccatgca accgtctggc gcaacaacag atgctggtgg tgtggactgg
     1561 cttaattcag cgccatgctc agcttgcgcc ggtaggtcgc caccagctgc gcttgcgggt
     1621 ccagttgacg gataggaaat tcaaccgcat gaggccaagt atggcacgcg ttttgggcct
     1681 cgaaacacgt tcgacacaag cagtttgagc gctcgcttgc cgctcaatgg caagagcttg
     1741 ggaggcaata tgcgtttgga tctggcgcgc acgcagctac tcaccgcagc gattgatacc
     1801 gccgcttcac tgggtcacca attgacgcgc tggtctacct cgtaatcggg tgcgggttgc
     1861 gctgccaaat cccaatccgg ctcgccttgc accccgtcat ccaccggcgc atcacactca
     1921 acccacagcg gtggcccgcg tgctggcgcg atgtgtgggg gttcggactc taccccgatg
     1981 tgttcgagta tttgccggat atcgacactg tgggtgatga acgcaatcag gcgcatctgc
     2041 ccaccgcaca tggggcacag cagcgggaag acttcgtaga ttctggcaat cagcaccgcc
     2101 cacaggtagt gcgctgggcg cttg
//
LOCUS       DAOSLL010000695         2122 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_45044_length_2122_cov_3.321239, whole genome shotgun sequence.
ACCESSION   DAOSLL010000695 DAOSLL010000000
VERSION     DAOSLL010000695.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45044_length_2122_cov_3.321239"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            270..1466
                     /locus_tag="PLB25_21040"
     CDS             270..1466
                     /locus_tag="PLB25_21040"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HOE44092.1"
                     /translation="MSLFSAVEMAPRDPILGLNEQFSADSNPNKVNLGVGVYFDDTGK
                     LPLLQCVQAFEKTMMDKPTAHGYLPIDGIAAYDAAVKALVFGVESEAVKSGRVATVQC
                     LGGTGGLKVGADFLKRLNPAAKVLISDPSWDNHRALFANAGFAVENYRYYAAATRSID
                     FAGMLADLNAAPTGTVVVLHACCHNPTGYDLSPAQWDMVIEVIKAKALTPFLDMAYQG
                     FSQGIAEDGAVIGKFVAAGLDFLVATSFSKSFSLYGERVGALSILCTSKEEANRVLSQ
                     LKIVIRTSYSNPPTHGGAIVAGVLANPELRALWEQELGEMRVRIKAMRQKLVDGLKAA
                     GVKQDMGFITDQIGMFSYSGLSKDQMVRLRNEFGVYGTDTGRMCVAALNSRNLDHVCA
                     SIAKVI"
     gene            1547..>2122
                     /locus_tag="PLB25_21045"
     CDS             1547..>2122
                     /locus_tag="PLB25_21045"
                     /inference="COORDINATES: protein motif:HMM:NF022187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HOE44093.1"
                     /translation="MKTLNIIGAGRVGQTLGRLFHVTGIYQLQGVLTRSGASVQQALG
                     FIGAGQACQALVDMPAADVWMLTVPDSHIAPVAQALAGLARRPALVFHCSGALGSDQL
                     DPLRQIGWHVASAHPLMSFARPDLALTQFAGTPCALEGDATALAELEAALIPLCARSF
                     VLAAQDKLLYHAGAVFASNFLPVLQSVAEQLW"
BASE COUNT          420 a          593 c          641 g          468 t
ORIGIN      
        1 agttcgacct cggtgtgact tgatatgtaa ttgttttgat ggaactaact actagcgtac
       61 caaggacttt ccaaaacagg ttcattgatc ttcctccagg cagggtcatc aacgaccgaa
      121 tcatacaaac ctgagtgggc caattcgaat ctggatgctg ggtgatgcgg ctggggagcc
      181 aggcacctgc gggcatcggt aaaatccccc cctgcccata tccagcgggc ccggaatttt
      241 ggttttttta ctcctacagg actctactca tgtcactttt ttctgccgtc gaaatggctc
      301 cccgcgaccc gattctgggg ctgaatgaac aattcagtgc cgacagcaac cccaacaagg
      361 tcaatctcgg tgtgggcgtt tactttgacg acaccggcaa gttgcccctg ctgcaatgcg
      421 tgcaggcgtt tgaaaagacc atgatggaca agccaactgc acacggttac ctgccaattg
      481 acggcattgc ggcttatgac gcagcggtca aagccttggt gtttggtgtc gagagtgagg
      541 cggtcaaatc aggccgagtg gccaccgttc agtgcctggg cggcaccggt ggtctgaagg
      601 tgggggcgga ttttttgaag cggcttaacc cggctgccaa ggttttgatc agcgacccga
      661 gttgggacaa ccaccgtgca ctgtttgcca atgccggttt tgcggtggag aactaccgct
      721 attacgctgc cgcgacccgc agcattgact ttgccggcat gctggccgac ctgaacgcgg
      781 ccccaaccgg caccgtggtg gtgttgcatg cctgctgtca caacccgacc ggatatgacc
      841 tcagcccggc gcagtgggac atggtgattg aagtgatcaa ggcgaaggcc ctgacgccat
      901 tcctggatat ggcttaccag ggctttagcc agggtattgc cgaggacggt gcggtaattg
      961 gcaagtttgt cgccgccggg ctggacttcc tggttgccac ttctttcagc aagagcttca
     1021 gcctgtatgg cgagcgcgtg ggtgccttga gcattctgtg caccagcaag gaagaagcca
     1081 accgggtgct gagccagctc aaaatcgtca tccgcaccag ctacagcaac ccgccaactc
     1141 acggtggcgc gattgtggca ggcgtgctgg ccaacccaga actgcgtgcg ttgtgggaac
     1201 aagagctggg tgaaatgcgc gttcgcatca aggcaatgcg ccagaagctg gttgacggcc
     1261 tgaaggccgc cggtgtcaag caggacatgg gttttatcac cgaccagatt ggcatgttca
     1321 gctattctgg cttgtcgaaa gaccaaatgg ttcggctgcg caatgaattt ggtgtctacg
     1381 gtaccgacac cggtcgcatg tgcgtggctg cgctcaacag cagaaacctg gaccacgtct
     1441 gcgccagtat cgccaaagtg atttgagtca ctgggttatg taagaaatcg cctcgcagac
     1501 cgcatgggat aagcgtaaag tgctattttt atagcagcat gtcggtatga aaaccctcaa
     1561 catcattggt gctggccggg ttggtcagac gctggggcgg ctgtttcacg ttaccggcat
     1621 ctaccaactg caaggcgtgt tgacccgctc cggggcatcg gtgcagcagg cgctgggatt
     1681 tatcggggct ggccaggcct gccaggcgct ggtagacatg ccggcggcgg atgtctggat
     1741 gctgacggtg ccggattcac acattgcccc ggtcgcgcag gcactggctg gtctggcacg
     1801 ccgccctgcc ctggtgtttc actgcagcgg cgcgctcggc tcagaccagc ttgatccgtt
     1861 gcggcaaatc ggctggcatg tggccagcgc ccatcccttg atgagctttg cccggcccga
     1921 cctggcgctg acccaatttg ccggaacacc ttgcgccctg gaaggcgacg cgacggcgct
     1981 ggccgaactt gaagccgcct tgatcccgct ctgtgctcgc tcttttgtgc tggcagcgca
     2041 ggacaaattg ctctaccacg ctggggcggt gtttgccagc aactttctgc cagtgctgca
     2101 aagcgtcgcc gagcagcttt gg
//
LOCUS       DAOSLL010000696         2105 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_45538_length_2105_cov_4.482439, whole genome shotgun sequence.
ACCESSION   DAOSLL010000696 DAOSLL010000000
VERSION     DAOSLL010000696.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45538_length_2105_cov_4.482439"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="PLB25_21050"
     CDS             <1..725
                     /locus_tag="PLB25_21050"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013722203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HOE44094.1"
                     /translation="ITFVTSEPYIGHLGLGGVGDSKSMLESEMRNRDMKWITNAKTTK
                     VEQGKIFVTQIDDQGNLLKEHEVAFKLAMMLPAFKGVDAVAAVPNLVNPRGMVLIDEF
                     QRSKAYRNIFSAGVCVAIPPVEVTPVPTGTPKTGYMIESMVGAICHNIADELVGKPAT
                     AKGTWNAVCLADMGDTGAAFVALPQIPPRNVNWFKKGKWVHLAKIAFEKYFIRKIKKG
                     TSEPIYEKYLFSLLGINRVEKQ"
     gene            752..976
                     /locus_tag="PLB25_21055"
     CDS             752..976
                     /locus_tag="PLB25_21055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011466021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HOE44095.1"
                     /translation="MTVQRYIFVIAGLFIMFSLALGVEGSPLFVSKWALAFTAFVGAN
                     LFQSGFTNFCPMALILKKLGVPEATVSCAN"
     gene            1018..1308
                     /locus_tag="PLB25_21060"
     CDS             1018..1308
                     /locus_tag="PLB25_21060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018914562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensing transcriptional repressor"
                     /protein_id="HOE44096.1"
                     /translation="MAVLTSETDRTDILNRLRRAEGQIRGVQRMIEEGESCLKVGQQV
                     SAVRKALDSTYLRMTMCYLQQELSNCLGPEDLQNPGLDNLLKEMESLLARIN"
     gene            complement(1374..>2105)
                     /gene="mnmA"
                     /locus_tag="PLB25_21065"
     CDS             complement(1374..>2105)
                     /gene="mnmA"
                     /locus_tag="PLB25_21065"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019576968.1"
                     /note="catalyzes a sulfuration reaction to synthesize
                     2-thiouridine at the U34 position of tRNAs; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HOE44097.1"
                     /translation="RFNPDTACHELLKGLDAGKDQSYFLHRLTQAQLAKSLFPIGDLQ
                     KPEVRRLAAEIGLPNAKKKDSTGICFIGERPFRDFLNRYIAKQPGPVKNDKGQTIAEH
                     VGLSFYTLGQRQGLGIGGLKAKGAQRGGGDHAPWFVARKDIANNTLWVVQGHDHPWLL
                     SCELQAQDASWVAGLPPASGPAAAKARYRQADAPCRLLGGTAQSFRLSFTEPQWAVTP
                     GQSAVLYKGEICLGGGVISAAPEAH"
BASE COUNT          465 a          574 c          612 g          454 t
ORIGIN      
        1 cgatcacctt tgtcaccagc gagccctaca ttggtcacct gggcttaggc ggtgtcggcg
       61 acagcaagtc gatgctggag tcagaaatgc gcaatcgcga catgaaatgg atcaccaatg
      121 ccaagaccac caaggtggag cagggcaaga tctttgtcac ccagattgac gatcagggca
      181 atctgctcaa agagcatgaa gtggccttca agctggccat gatgctgccc gctttcaaag
      241 gtgtcgatgc agtggcggcg gtccccaatc tggtcaaccc gcgcggcatg gtgctgattg
      301 acgagttcca gcgcagcaag gcttaccgca atattttctc ggctggtgtg tgtgtcgcga
      361 ttcccccggt ggaagtgaca ccggtgccaa ctggcacccc caagaccggc tacatgatcg
      421 agagcatggt gggtgccatt tgccacaaca ttgccgacga attggtcggc aaaccggcta
      481 ccgccaaggg cacctggaac gccgtctgcc tggcggacat gggcgacacc ggtgccgcct
      541 ttgtggcact gccgcagatt ccgccgcgca acgtgaactg gttcaagaag ggcaagtggg
      601 tgcatttggc gaaaattgcg tttgaaaagt acttcattcg caaaatcaaa aaaggcacat
      661 ccgagccgat ttacgagaag tacctgttca gtttgctggg catcaaccgc gtcgagaaac
      721 agtaattttt aaatcagaag gagttatcac catgaccgtg cagcgctaca tattcgttat
      781 tgcgggcctt ttcatcatgt tttcactggc tttgggggtt gaaggcagtc cgctgttcgt
      841 cagcaaatgg gcgctggcgt tcacggcctt tgtcggcgcg aatctgtttc agtcgggctt
      901 taccaatttt tgtccgatgg cgctcattct gaaaaagctc ggcgtgccag aggcaaccgt
      961 gagttgtgcc aattagccag tgatgctgaa cctcaaccat tcaaccagga ggctttgatg
     1021 gccgtgctga ccagtgaaac agatcgtacc gacatactga accggctgcg ccgtgccgaa
     1081 gggcagattc ggggcgtaca gcgcatgatt gaagaaggag aaagctgcct gaaggtcggc
     1141 cagcaggtct cagcagtgcg caaggcgctt gacagcacct acctgcgcat gaccatgtgc
     1201 tacttgcaac aggaactaag caactgcctg gggccagagg atttgcaaaa tcccggtctt
     1261 gacaacctgc tcaaagagat ggaaagcctg cttgcccgca tcaattagct tttatgcaca
     1321 aaaacaggtt ctagccctta tggagcgtgc gctaccagct atattaataa tagttaatga
     1381 gcttccgggg cggctgaaat tacaccgcct ccaaggcaaa tttcgccttt gtaaagcaca
     1441 gcagattgcc ccggtgtcac cgcccattgc ggttcggtga aactcaggcg gaagctttgc
     1501 gctgtgccac cgagcaagcg gcagggcgcg tcggcctggc ggtaacgggc ctttgccgcc
     1561 gccggaccgc tggcgggtgg cagtcccgct acccaactgg catcctgcgc ctgaagctca
     1621 catgacagca gccaagggtg gtcgtggcct tgcaccaccc acagcgtgtt gtttgcgatg
     1681 tctttgcggg caacaaacca gggggcgtga tcgccaccgc cgcgctgcgc ccccttggcc
     1741 ttaaggccgc caatgcccag gccctggcgt tggccaaggg tgtaaaagct cagtcccaca
     1801 tgttcggcaa tggtttggcc tttgtcgttt ttgaccgggc cgggctgttt ggcgatgtag
     1861 cggttcagaa aatcacgaaa cggtcgctcg ccaatgaaac agatgccggt ggaatccttc
     1921 tttttggcgt tcggcagacc gatttcggcg gccaggcggc gcacctctgg tttttgcaag
     1981 tcgccgatgg gaaacaacga cttggcgagc tgcgcctggg tcaagcggtg gagaaaatag
     2041 ctttgatcct tgcccgcgtc caaccctttc aacaattcat ggcaagcggt gtcagggttg
     2101 aagcg
//
LOCUS       DAOSLL010000697         2104 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_45573_length_2104_cov_3.916545, whole genome shotgun sequence.
ACCESSION   DAOSLL010000697 DAOSLL010000000
VERSION     DAOSLL010000697.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45573_length_2104_cov_3.916545"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..965)
                     /locus_tag="PLB25_21070"
     CDS             complement(78..965)
                     /locus_tag="PLB25_21070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HOE44098.1"
                     /translation="MKVKFWGVRGSIASPGPKTVRYGGNTTCIEIRTDNNELIILDAG
                     TGIFPLSQTLLAELPVTANVLITHSHWDHIQGLPFFIPNFIPGNTLRLHGSFDPVSGK
                     GVEQVMSVQLQYSYFPVREAEMKARIEYVTLAPNETIQIGSATVTPCLLNHPVINFGY
                     RIECNGKSVFFTGDHEPPYNIYAPEDDGFDEYQVFVDEKKAAIDAILSGVDVLIADCS
                     YTEDEYPAKIGWGHGTFKSSMLSAQKAGVKVLFCTHHEPTRSDDALEAAFDAAVKTNA
                     AETQGLDIRLAREGDVYEF"
     gene            complement(978..1820)
                     /locus_tag="PLB25_21075"
     CDS             complement(978..1820)
                     /locus_tag="PLB25_21075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDOD domain-containing protein"
                     /protein_id="HOE44099.1"
                     /translation="MSPELSQKLAAAVEGMPAFPKSVQKILELTRDVNSTPKDLVEVI
                     DKDPVVTVKTLKVVNSAYYSLPKQITSIGHAVVYLGFNTIKNLALSIAAIGMMPKDNA
                     SGFDVQQYLLHSLATAGLAKQLALKMDDVDPMDCFIGGLLHDFGKVVFAQFMPQEFRR
                     ALQHCQSNNVPLHVALQDAIGTDHFVVGAMLVEKWRFAPKLIETIRYQHPSNFKDTEM
                     IACVFGGNQISKKLKFGFGGNTSVDEFPASVQKRLGGTLDEVIASMGDLNPLFEEAKL
                     FAKL"
     gene            complement(1890..>2104)
                     /locus_tag="PLB25_21080"
     CDS             complement(1890..>2104)
                     /locus_tag="PLB25_21080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009858803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HOE44100.1"
                     /translation="SGGQIWKGTFGVMRDGLPVIGQSALPGVWLNLGHGPNGWGMACG
                     AARCLADSMTHRTPPLSLEKFSPLRF"
BASE COUNT          473 a          544 c          569 g          518 t
ORIGIN      
        1 ccaggcgccg ggttgcagcg ctgttgtgca ggattcggat tcgcgctgac cgcaaaccat
       61 ccgggccaac gatgcgttca gaactcgtag acatcaccct ctcgcgccag gcgaatgtcc
      121 aggccctgtg tttctgctgc gttggtcttg acggcggcgt cgaaggctgc ctccaatgcg
      181 tcgtcgctgc gcgtaggttc gtggtgggta caaaacagca ctttgacccc tgccttttgg
      241 gcgctaagca tgctggactt gaaggtcccg tgtccccagc caatcttggc cggatactca
      301 tcttctgtgt atgagcaatc tgcgatcagg acatcaaccc cggacagaat tgcatcaatc
      361 gcggcttttt tttcgtcgac aaacacctgg tattcatcga agccatcgtc ttccggcgca
      421 tagatgttgt agggcggttc atggtctccg gtaaaaaata cggacttccc attgcattca
      481 atacgatagc caaagttgat caccggatga tttagcaagc agggcgtgac cgtggctgag
      541 ccaatctgaa tcgtctcgtt cggtgcaagg gtcacatact caatgcgcgc cttcatctcg
      601 gcttcccgca ctgggaagta gctgtattgc agttgcacgg acatgacctg ctccacccct
      661 ttgccagaga cagggtcaaa actgccgtga aggcgtagcg tattgcccgg aatgaaattt
      721 gggatgaaga atggcaggcc ctggatgtgg tcccagtggg aatgtgtaat cagcacattg
      781 gctgtgacgg ggagctcggc caggagcgtt tgtgacagag ggaaaatgcc ggtgccagca
      841 tccagaatga tcagttcgtt attgtctgtg cgtatctcga tgcaggtcgt gtttccgcca
      901 taacgaacgg tcttgggacc cggagaggcg atggagccgc gaacacccca aaactttact
      961 ttcatgtgtt actcctgcta cagcttggca aatagcttgg cttcttcaaa taacgggttg
     1021 aggtctccca tagacgcaat gacctcatcc agagtgccac ccaagcgctt ctgcacggat
     1081 gccggaaact cgtccacact cgtattgccg ccgaaaccaa atttcagttt tttgctgatt
     1141 tgatttccgc caaatacaca agcaatcatc tcggtatctt tgaagttcga cggatgctgg
     1201 taccggatag tctcaatcag ttttggtgca aatcgccatt tttcgaccag catggcacca
     1261 acaacaaaat ggtctgtgcc aatggcatcc tgtagtgcaa catgaagagg aacattgttg
     1321 ctctggcagt gctggagggc ccgacggaat tcctggggca taaattgtgc aaacaccact
     1381 ttgccaaaat catgcaagag tccaccaatg aagcagtcca ttggatcaac atcgtccatc
     1441 ttgagtgcca actgctttgc cagcccagct gtagccaggg agtggagaag gtactgctga
     1501 acatcaaatc ctgaagcgtt gtccttgggc atcatgccaa tggctgcgat actcagagcc
     1561 aggttcttga tggtattgaa tcccaggtac acaactgcgt gtccgatcga tgtgatttgt
     1621 ttgggaagac tgtagtaggc agagttgacg accttgaggg ttttcactgt gacaacagga
     1681 tctttgtcga tgacttcgac caggtccttg ggagtgctgt tgacatcccg agtcaattcc
     1741 aggatttttt gcacgctttt gggaaaggca ggcatgcctt ccaccgctgc agcgagtttt
     1801 tgcgaaagtt ctggactcat ggcgtttttc ttatgctaat tggcttgcat tgtgtcagtt
     1861 cggcagggtt tcgggaaaaa actcacacct tagaagcgca atgggctgaa cttttccagc
     1921 gagaggggtg gggtacggtg agtcatcgag tctgctaggc atcgcgcagc gccgcaggcc
     1981 attccccatc cgttggggcc gtgacccaga ttgagccaaa ctccaggcaa ggcgctttgc
     2041 ccgataacag gcaagccgtc gcgcatcacg ccaaaagtgc ccttccagat ttgtcctccg
     2101 ctgg
//
LOCUS       DAOSLL010000698         2100 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_45702_length_2100_cov_3.111491, whole genome shotgun sequence.
ACCESSION   DAOSLL010000698 DAOSLL010000000
VERSION     DAOSLL010000698.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2100)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45702_length_2100_cov_3.111491"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            354..485
                     /locus_tag="PLB25_21085"
     CDS             354..485
                     /locus_tag="PLB25_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44101.1"
                     /translation="MRLYIAESETPNALTANTLRKSDAGEDVFYASGASDLFKQLGI"
     gene            554..685
                     /locus_tag="PLB25_21090"
     CDS             554..685
                     /locus_tag="PLB25_21090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44102.1"
                     /translation="MEKLRKVIDMLLTDQPQPNELCDRPRKAGGNPGVIFTSNQTGY"
     gene            682..780
                     /locus_tag="PLB25_21095"
     CDS             682..780
                     /locus_tag="PLB25_21095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44103.1"
                     /translation="MIYLVDDEILRLERTGTNADLFGYAHNKRLQK"
     gene            complement(771..2042)
                     /locus_tag="PLB25_21100"
     CDS             complement(771..2042)
                     /locus_tag="PLB25_21100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44104.1"
                     /translation="MHHFTRFALCCGLALAAACALPALAADTELDALGLESAPEVKPE
                     ATRSTKLFVEGAVGNASQRYSPDSRNIGRASIDFSHVARLAPGLRAVVSDRLDYFDPA
                     SAGSDASVNSLREAYVSWQPEGGHTVLELGRINLRYGPAYGYNPTDFFRDGSLRVITT
                     ADPFALRENRMGTVMLRGQYLWDKGALSLAVSPKLADAPSASGWNLDLGSTNYRDRYL
                     VALSTQFTERVSSQTLLYQESGRSPTVGFNMTALLSDAATAHMELTRGSEPDLLSRVM
                     TAPATDVARNRFVGGVTYTTLGKMSLTAEYQYNGFGLNHTDWAALGAAPLAQLSYLSS
                     ALRLQELAPRQAYLIYVTQKSLMLKDLDLTAYLRLNPGDYSRLAWIELRHHWPNFDLT
                     LQLQQNIGKPTSEFGILPDRRVVQVLGSYYF"
BASE COUNT          491 a          585 c          600 g          424 t
ORIGIN      
        1 aacttgactt tcatccacat gtgcacatgg ccatgcctgc ggcagcactg gatgctgaca
       61 gaaagatatg gcgcactttg cgcaagagcg ccaagggggt cagtttccat gagcgccaaa
      121 gcagaatcac gccatcgaaa ttgacggggt gaaggtgcaa aataaatcgt ctcagccagc
      181 gctgttgcgg ggtggtttac tccgcgtagc ggcttcgtcc aacccacatg agtgatttca
      241 ccatgcctaa agaagtacaa atgtcgatca agctggagtc aacgctacgc aaccagttca
      301 tggctgcggt agctctgcag cattgaccgg ccgcgcagat cgtccgcgac ctgatgcgac
      361 tttacatcgc cgaaagcgaa acacccaatg cactgaccgc caacacactt cgcaagagcg
      421 atgcaggcga ggacgttttt tacgcttccg gtgcatctga tctgtttaaa caactgggta
      481 tttgatgcgc ttggcaagct actcaggcca gttttagcgt gacatcaaac tggcagaaag
      541 acgtggcaaa aatatggaga aactgcgcaa ggtgatcgac atgctattga cagatcaacc
      601 ccagcccaat gagttgtgtg accgcccccg aaaggcaggt ggcaaccccg gcgtgatctt
      661 cacatcgaac cagactggct attgatctat ctggttgatg atgaaatcct tcgcctggag
      721 cgcaccggca caaacgctga tctttttggc tatgcgcaca acaagcgcct tcagaaataa
      781 taactaccca gcacctgcac cacgcggcgg tcgggcagaa tgccaaattc gctggtcggt
      841 ttgccaatgt tctgctgcaa ctgcaaggtc agatcaaagt ttggccagtg gtgacgcaat
      901 tcaatccagg ccaggcggct gtagtcacca gggtttaatc gcagataggc cgtcaggtcc
      961 aggtctttga gcatcaaact cttttgtgtg acgtaaatca ggtaagcctg acggggtgcc
     1021 aattcttgca gccgaagcgc tgagctcagg taggacaact gggccaatgg cgctgcaccc
     1081 aacgcggccc agtcggtgtg gttgaggcca aagccgttgt actggtattc agccgtcaaa
     1141 gacatcttgc ccagcgtggt gtaggtcaca ccaccgacaa aacggttgcg ggcgacatcg
     1201 gttgccggtg cggtcatgac ccgactcagc aaatcaggct cactgccacg ggtcaattcc
     1261 atgtgcgcgg tggcggcatc tgacagcaat gccgtcatgt tgaagcccac cgtgggcgag
     1321 cggccgcttt cctgatacag caaggtttgg ctgctgacac gctcggtaaa ctgggtgctg
     1381 agcgccacca gataacgatc ccggtagttg gtagagccca ggtcaaggtt ccagccgctg
     1441 gcactgggcg catccgccag tttgggtgac accgccaagg acagcgcacc cttgtcccac
     1501 aggtattgcc cgcgcaacat cacggtgccc atgcggtttt cgcgcagcgc aaacgggtcg
     1561 gccgtggtga tcacgcgcaa gctgccgtcg cgaaaaaagt cggtcgggtt gtagccgtaa
     1621 gcaggtccat agcgcaggtt gatacgaccc aactccagca ccgtgtgacc gccctcgggc
     1681 tgccagctca cataggcttc acgcagacta ttgacactgg catcgctgcc ggcactggct
     1741 gggtcaaaat aatcaagccg atcagacacc acggcgcgca ggcctggggc cagccgggcg
     1801 acatgcgaaa aatctattga agcacgcccg atgttgcggc tgtcgggcga gtagcgctgg
     1861 ctggcattgc ccactgcacc ttcaacaaac agcttggtgc tgcgggtcgc ttcaggtttg
     1921 acttcgggtg cgctttcaag acccaatgca tccagctcgg tatctgctgc caaagctggc
     1981 aaggcacacg ctgctgccag tgctaggcca caacataagg cgaagcgggt gaagtggtgc
     2041 atagatttag tctggttgaa agcggggcaa aaagtcgcgc tgcatccagg tgtctggaat
//
LOCUS       DAOSLL010000699         2078 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_46376_length_2078_cov_4.780524, whole genome shotgun sequence.
ACCESSION   DAOSLL010000699 DAOSLL010000000
VERSION     DAOSLL010000699.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46376_length_2078_cov_4.780524"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="PLB25_21105"
     CDS             complement(<1..656)
                     /locus_tag="PLB25_21105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HOE44105.1"
                     /translation="MHATDNPARILVVDDEPDLRALYELTLLREGYRIDTAENLLQAQ
                     ALLAEHFFDVMITDMRLPDGLGMSLIVDLKRKQREERCVVITAYGSTQNAVEALKAGA
                     FDYLTKPVDLKQFRSVIATAVRSTPATLTSDQVNLVKEAPLAATGALGQRALERLVGT
                     SDCMCQIKERILKVATSMAPVMVTGESGTGKELVAYAIHTNSHRAKGPWVAVNCGAIP
                     "
     gene            complement(661..>2078)
                     /locus_tag="PLB25_21110"
     CDS             complement(661..>2078)
                     /locus_tag="PLB25_21110"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE44106.1"
                     /translation="TFTTLQMFQGSGINYAPLYALPVLMASFLGSMLLAMAAAAVVTF
                     LLFAHAAWLSLYAPGDINTYFLQAALTGATCLAIAFIASQLAMRLASVELLAQHSQLA
                     ATVQRQVNELVIASLTDAILVVDQDGQIRSGNPAARYVLGTLELHQDAELNLNERIAW
                     QALSDVVSTTFVTRQARQSDITLDHVGQGQRSLRVRTQLTEPLDQDAQVLCVVFMQDQ
                     REIEARVRAEKLASMGRMSAAVAHEIRNPLAAITQANALLMEDLSDPAQLRLANMVQQ
                     NALRLEQIVHDVLHLAQASAPDLIDAQQSLNLPETTQRICRDWKNQHAVSDELLVDLR
                     DGSIAVNFDPEHLRRILINLLDNALRYASRQTACIQISTTVNQTSVTKVSLFVWSDGA
                     PLEPSVERHLFEPFFSSESRSSGLGLYICRELCASHGASIVYGRTARTINGQVALGNE
                     FSIVFKTQLPRLANPLKQEPA"
BASE COUNT          475 a          574 c          575 g          454 t
ORIGIN      
        1 tcgggaatgg cgccacagtt cacggcaacc catggtcctt tggcgcgatg gctgttggta
       61 tggatcgcat aggcgaccag ttctttacca gtacctgact cgccggtcac catcaccggt
      121 gccatgctgg tagcgacctt cagaattcgt tccttgatct ggcacatgca atcagatgtc
      181 ccaaccagcc gctcgagcgc tcgttgcccc agagcaccgg tagcagccaa aggcgcttcc
      241 ttgaccaggt tgacttgatc cgaagtgagt gtggcagggg tgctgcgcac cgccgtggcg
      301 atgacgcttc gaaactgttt caggtcaacc ggcttggtca ggtaatcaaa cgcaccggct
      361 ttcagcgcct cgactgcatt ctgggttgat ccataagcag tgatgacaac acagcgttct
      421 tctcgctgct tgcgtttgag gtcaacaatc agcgacattc ccagtccatc aggcaaccgc
      481 atatcggtaa tcatgacatc aaagaaatgt tcggccaaaa gggcttgggc ttgcagcaaa
      541 ttctctgcgg tgtcgattcg ataaccttct cgcaacaagg tcaactcata aagcgcgcgc
      601 aaatccggtt catcgtcaac caccagaatg cgggcagggt tgtcagtagc gtgcatggtg
      661 tcaggcgggt tcctgcttga gtggattcgc taatcggggt aattgagttt taaaaacaat
      721 gctgaattca ttgcccaagg ctacttgtcc gttgattgtt cgggcagtgc gcccataaac
      781 gatggacgca ccatgactgg cgcatagctc acggcaaata tacaacccca ggccactgga
      841 tcgactctcg gatgaaaaaa agggttcaaa caggtgccgc tcgacagatg gctccagggg
      901 tgcgccatca ctccagacga acagactgac ctttgtcacc gaggtttggt taaccgtggt
      961 gctgatctgg atacaggcgg tttggcggct ggcgtaacgc agggcattgt ccagcaggtt
     1021 gatcaaaatg cgccgaagat gttcagggtc gaagttgaca gcgatactgc catcgcgcaa
     1081 atccaccagc aattcgtctg aaaccgcgtg ctggtttttc cagtcacggc aaatccgttg
     1141 ggttgtctcg ggcagattca gactttgttg cgcgtcaatg aggtcgggtg ctgatgcctg
     1201 tgccaggtgc aatacatcat gcacgatctg ctcaagacgc agcgcgtttt gctgcaccat
     1261 gttggccagc cgcagttgtg ccggatcgct caagtcttcc atgagcagtg cattggcctg
     1321 agtgatggcc gcgagcggat tgcgaatctc gtgtgcgaca gcagccgaca tgcgccccat
     1381 gctcgccagt ttctctgcac gtacgcgtgc ttcaatttca cgttggtcct gcataaagac
     1441 aacacacagc acctgtgcgt cctgatcaag cggctcggtg agttgggtac gtacccgaag
     1501 gctgcgctgg ccctggccga cgtggtcaag ggttatgtcc gattgtcggg cctgccgggt
     1561 gacgaaggtg gtcgaaacaa cgtccgacaa tgcctgccag gcaatgcgct cattcaaatt
     1621 caactccgca tcctgatgca gttcaagtgt gcccagcaca taccgcgctg ccggattgcc
     1681 cgagcggatt tgaccatctt gatcgaccac caggatggca tctgtcaggg acgcaatgac
     1741 caactcattc acctggcgtt gcacagtcgc tgccaactgg ctgtgctgtg ccagaagctc
     1801 tacgctggcg aggcgcatgg ctaactgact tgctataaac gctatagcta aacaagtagc
     1861 acccgtaagg gcagcctgca gaaaataggt gttgatgtcg ccgggggcgt acagcgacaa
     1921 ccacgctgca tgggcaaaca acagaaatgt caccaccgcc gccgccgcca ttgccagaag
     1981 cattgaaccc aaaaaactgg ccatcaagac cggcaaggca tacagcgggg catagttgat
     2041 gccactcccc tgaaacattt gcaaagtcgt gaacgtga
//
LOCUS       DAOSLL010000700         2039 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_47604_length_2039_cov_2.074597, whole genome shotgun sequence.
ACCESSION   DAOSLL010000700 DAOSLL010000000
VERSION     DAOSLL010000700.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47604_length_2039_cov_2.074597"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="PLB25_21115"
     CDS             <1..210
                     /locus_tag="PLB25_21115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44107.1"
                     /translation="EDNGLGLGNSTSAGSGTGLVNTRNRLKAIYGEQAKLVITNRTAG
                     GVSASIRIVQRTQDGDLHGEGSIGR"
     gene            185..955
                     /locus_tag="PLB25_21120"
     CDS             185..955
                     /locus_tag="PLB25_21120"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HOE44108.1"
                     /translation="MAKAVLADDEPIMRAALREQLEMLWPELLIVAEADDGPIALQKI
                     ETLKPDVAFLDIRMPGLSGLEVAKAITQSTSIVFVTAFDSHALEAFEANAVDYVLKPV
                     DPVRMARVIAKVKKNLSDGTPADIAQLILTLERAGLSTTGKSQAASISRMEWLQVAVG
                     TQIRMLHVSDVIYFESDTKYTRVVAEDCDGLIRISLKELLESLDSSTFLQTHRSSLVN
                     RRFVHSVHRKGEVVEIELKGRPERLKVSIANHHIFKAM"
     gene            1129..1605
                     /locus_tag="PLB25_21125"
     CDS             1129..1605
                     /locus_tag="PLB25_21125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44109.1"
                     /translation="MKVRPRQTPSAILSTGRLGKQIVNAALASLLVVTATATVADVAS
                     RVQESNSSAHHGVLAFVRDASPQGLRLEQHFFGQGLRKVSAVAPDFQIQIVESEDIPG
                     GALPGSFVVLTRAGDRVVALQHGCKTETDLRALVADARGALGNSVPVVAAYFEYDH"
     gene            1621..>2039
                     /locus_tag="PLB25_21130"
     CDS             1621..>2039
                     /locus_tag="PLB25_21130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44110.1"
                     /translation="MQSISAAGLDGHWVDVITWIFVLANTGRLLAYLPQFVSAWNCPH
                     GAKSVSILTWSYFTFAHCTALMYALHVLHDSKSVWIFAGNLVVTLCLVTLLLWKRVTF
                     IHAVRCSFGGVDSEIAYGNRNLNLPRYEAASNDDRFGT"
BASE COUNT          481 a          507 c          560 g          491 t
ORIGIN      
        1 gaagacaatg ggcttgggtt aggcaattca accagtgctg gcagtggaac tgggcttgtc
       61 aatacgcgca accggctgaa ggctatttat ggcgagcaag ccaaattggt tatcaccaac
      121 cgtaccgccg gtggcgtgtc cgcttccatt cgtatagttc agagaacaca agacggagac
      181 ttgcatggcg aaggcagtat tggcagatga tgaacccatc atgcgcgcgg cattgcgcga
      241 gcaactcgag atgctctggc ccgagttgtt gatcgttgca gaagctgacg atggaccgat
      301 cgccctgcaa aagatagaaa ccttaaaacc cgatgtggct tttctggata tccgtatgcc
      361 tgggctcagc ggtcttgaag ttgcaaaagc cataacgcag tcgacgagca tcgtatttgt
      421 gacagcgttt gattcgcatg cgctggaagc gtttgaggcc aatgcagtcg actatgtcct
      481 caagccagta gaccctgtac gcatggcaag ggtcattgcc aaagtcaaga aaaacctgag
      541 tgatggaaca cctgccgaca tcgctcagct tatcctgact ctcgaacgcg ctggtctctc
      601 aacaacgggc aagtctcagg ccgcatcgat ctctcgcatg gaatggctac aagttgcagt
      661 gggcacacaa atacgcatgc tccatgtcag cgatgtcatt tactttgaat ccgacacgaa
      721 atacacgcgt gttgtagctg aggattgcga tggactcatc cgcatttctc tcaaggaact
      781 gctggagtct ttggactcat cgacctttct gcaaacgcat cgaagctctt tggtgaatcg
      841 tcgattcgtt catagcgttc accgaaaggg ggaggttgtt gagatcgaat tgaagggccg
      901 ccctgagcgg ctcaaggtgt ctatcgcgaa tcaccacatt tttaaggcga tgtgaccagt
      961 cgtctatagc tgcgcattcg acaccaattt tcccaagctc gtcggttgtt ccccgagttc
     1021 gtcgcaattc ggatgaacga gatggatgtg acaaatagag ttacttcacc gatgcactca
     1081 tgcgagtgca aaggcctagc aaagtaacca accgaaccaa gggaaaacat gaaagtcaga
     1141 ccacgccaaa cacccagcgc catcctgtcc acggggcgtt taggaaagca gattgtcaac
     1201 gctgccctcg caagtcttct ggtagtcact gctactgcta ccgtggcgga cgtagcgtca
     1261 cgggtacagg aatccaactc ctccgcgcac catggtgtat tggcctttgt gagggatgcg
     1321 agtccgcaag gcctcagatt ggagcaacac ttctttggac aaggtttgcg aaaagtaagt
     1381 gctgtggcac cggacttcca gattcagatc gtggagtctg aagacattcc aggcggcgcg
     1441 cttccgggaa gtttcgtcgt tttgacgcga gcgggtgatc gggttgtggc gctgcagcat
     1501 ggttgcaaaa ctgagactga cttgcgagct ttggtcgcag atgcaagagg cgcgctgggc
     1561 aactcggtgc cggtagtcgc cgcgtatttt gagtacgacc actgatcgcg aggcaatact
     1621 atgcagagca tcagtgcagc aggattggac ggccattggg tcgatgtcat tacgtggatc
     1681 tttgtgcttg caaacacggg acgtttgctg gcctacttgc cacaatttgt atcggcttgg
     1741 aattgtcctc atggagcaaa gagcgtatcg atactgactt ggtcgtactt cacttttgca
     1801 cactgcaccg ctctcatgta tgccttgcat gtgttacacg actccaagtc cgtttggata
     1861 tttgccggaa atcttgtcgt gaccctgtgc ttggtgacgc tgctgctttg gaaacgagtg
     1921 accttcatcc atgcggtacg ttgctcgttc ggtggtgtgg attctgaaat agcatatggc
     1981 aacagaaatt tgaacttacc ccgatacgaa gccgcgtcga atgatgaccg ctttggcac
//
LOCUS       DAOSLL010000701         2034 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_47730_length_2034_cov_4.514907, whole genome shotgun sequence.
ACCESSION   DAOSLL010000701 DAOSLL010000000
VERSION     DAOSLL010000701.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_47730_length_2034_cov_4.514907"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1136)
                     /locus_tag="PLB25_21135"
     CDS             complement(<1..1136)
                     /locus_tag="PLB25_21135"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012206113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HOE44111.1"
                     /translation="MTNDDILAQFGPRESMEYDVVVVGGGPAGLSTAIRLKQLAAEKG
                     KEVSVVVLEKGSEPGAHILSGAVLDPQSLTELLPDWKAQGAPLNQPVTDEAMLFLSET
                     TGYRTPNMFLPKCSQNHGNYIISLGALTRWLATQAEALGVEIFPGFAAAQVLYNEAGQ
                     VTGVATGNMGVEKNGEPGDNFQLGMELLGKYTIFAEGSRGHLGKQIIARYKLDEGCDP
                     QSYAIGIKELWEVEPARHEPGLVLHTAGWPMDDKTYGGSFLYHMEDNKIAMGFITGLN
                     YSNPYLSPFEEFQRWKTHPNIRWYLENSKGQVTAKRIAYGARAITAGGILSLPKTVFP
                     GGALVGCDAGYLNVSRIKGTHAAIKSGMLAAEAAFEAVTAGRAQ"
     gene            1274..>2034
                     /locus_tag="PLB25_21140"
     CDS             1274..>2034
                     /locus_tag="PLB25_21140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007854458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HOE44112.1"
                     /translation="MAYSIDLAGRVALITGASSGLGAQFARTLSAAGAAVVLASRRIE
                     KLKDLRAQIDGEGGDAHVIELDVTDHDSIKSAVAHAETEVGSIDILVNNSGVSTTQRL
                     QDVGESDFDFTFDTNVKGAFFVAQEVGKRMLARSQGSAPGSFTGGRIVNVASMAGLRV
                     LPKIGVYAMSKAAVIQMTRAMALEWGKFGINVNAICPGYIDTELNHSHWQTEQGQKLV
                     QMLPRKRVGQPQDLDALIVMLCSDQSHFINGAVISA"
BASE COUNT          391 a          567 c          622 g          454 t
ORIGIN      
        1 tcttgtgccc ggccggccgt cacggcttca aaggcggcct cggcagccag catgcccgac
       61 ttgatcgctg cgtgcgtgcc cttgatgcgg ctgacattca ggtagcccgc atcgcaaccc
      121 accagcgcgc cacccggaaa caccgttttt ggcaaggaca aaatgccgcc agcggttatg
      181 gcgcgggcac cgtatgctat tctttttgca gttacttgac cttttgagtt ttccaggtac
      241 cagcggatgt tggggtgggt tttccagcgt tggaattcct caaacgggct caggtagggg
      301 ttgctgtagt tgaggccggt gataaagccc atggcgatct tgttgtcttc catgtggtac
      361 aggaaggagc cgccataggt cttgtcatcc atcggccagc cagcagtgtg cagcaccagg
      421 ccgggttcgt ggcgtgccgg ttcgacttcc cacagttcct tgatgccaat cgcgtagctt
      481 tgcgggtcgc agccctcgtc gagcttgtac ctggcaatga tttgtttacc caagtggccg
      541 cgcgagcctt cggcaaagat ggtgtatttg cccagcagtt ccatgccgag ctggaagttg
      601 tcgccgggtt cgccattttt ttcgacaccc atgttgccgg tcgcgacacc ggtgacctgg
      661 ccggcctcgt tgtagagcac ttgcgctgcc gcaaagccgg gaaaaatctc cacgccaagg
      721 gcttcagcct gggtcgccag ccagcgtgtc agtgcgccca ggctgatgat gtagttgcca
      781 tggttctggc tgcacttggg caaaaacatg ttgggggtgc ggtagccggt ggtctcgctc
      841 aaaaacagca tcgcctcgtc ggtaaccggc tggttcaaag gggcaccttg tgccttccag
      901 tcgggcaaca gttcggtcag cgactgcggg tcgagcaccg caccggacaa aatgtgagca
      961 ccgggctcag agcctttttc cagcaccact accgatactt ccttgccctt ttcggcagcg
     1021 agctgtttga gccggatcgc agtcgacagg cccgccgggc cacctccgac cacgacaacg
     1081 tcgtattcca ttgattctcg ggggccaaac tgggccagga tgtcgtcatt tgtcatggtg
     1141 gtactcgctt gataatcgga caagaaaatt gctcgggttt gcaacgattg acggttgata
     1201 tttaaaacta ttttaggcgt gtggaaatac gcataataga acggtcgttc tattcatcat
     1261 ggagatttga gatatggctt acagcattga cttggcgggc cgggtggcct tgattaccgg
     1321 cgcatcaagt ggtctggggg cccagtttgc ccgcaccctg tcggctgccg gggccgccgt
     1381 ggtgctggcc agcaggcgca ttgaaaagct caaggacttg cgcgcccaga ttgacggcga
     1441 gggcggcgat gcccatgtga ttgaacttga tgtgaccgat catgactcga tcaaatcggc
     1501 ggtggcacat gccgagaccg aagtcggctc gattgacatt ctggtcaaca actcgggggt
     1561 cagcaccacc cagcggctgc aggacgtggg cgagagcgac tttgatttca cctttgacac
     1621 caatgtcaaa ggggcttttt ttgtcgcgca agaggttggc aaacgcatgc tggcgcgctc
     1681 gcagggctca gcgccgggca gttttaccgg cgggcgcatc gtcaacgtgg cctccatggc
     1741 ggggctgcgg gtgttgccaa agattggcgt ttatgccatg agcaaggccg cagtcattca
     1801 gatgacccgc gcgatggcgc tggagtgggg caagtttggc atcaatgtca atgccatctg
     1861 ccccggctac atcgacaccg agttgaacca ctcgcactgg caaaccgagc aggggcaaaa
     1921 gctggtacag atgctgccgc gcaagcgcgt cgggcagccg caagacctcg acgcgctgat
     1981 cgtcatgctg tgctcggatc agagccattt catcaacggc gcagtgattt ctgc
//
LOCUS       DAOSLL010000702         2010 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_48501_length_2010_cov_2.800512, whole genome shotgun sequence.
ACCESSION   DAOSLL010000702 DAOSLL010000000
VERSION     DAOSLL010000702.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48501_length_2010_cov_2.800512"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..1499)
                     /gene="dbpA"
                     /locus_tag="PLB25_21145"
     CDS             complement(111..1499)
                     /gene="dbpA"
                     /locus_tag="PLB25_21145"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009519136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HOE44113.1"
                     /translation="MTNSEKIPFNTLPLNPATLANLEQLGYLSMTPIQAGSLPAALAG
                     KDLIAQAQTGSGKTAAFGLALLERLNPRRFAVQALVLCPTRELADQVTVEIRRLARAM
                     DNVKVVTLCGGVALRGQRASLENGAHIVVGTPGRVMDHLARGYLALDALNTLVLDEAD
                     RMLDMGFFDDIATVTKQCPPERQTLLFSATYPEGIEKLAKQFMRSPEQITVAPPATQS
                     LIEQRFYEVTRPTRFDVVARLLNHFRPVSTLAFCNTKQQCKDLVTYLQQQGFSALALY
                     GELEQRERDQVLVQFANRSTSILVATDVASRGIDIKELEAVINVDITPDPEVHVHRIG
                     RTGRAGETGLALSLAAMYEMGAVGKIEQYQKLDSVWHKIDELSPTGSGPLLPPMVTLQ
                     ILGGRKEKIRPGDVLGALTADAGYSREQIGKINVTEFTTFVAVQRAIALEAMQKLNAG
                     KIKGKSVKVRLL"
     gene            1639..>2010
                     /locus_tag="PLB25_21150"
     CDS             1639..>2010
                     /locus_tag="PLB25_21150"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011828906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HOE44114.1"
                     /translation="MLPLVLAALLTACGSAVVNPVSGQAERSVMSEQTEVAEGAKAHK
                     EVLQEYGVVKDARLQAYVNELGQRLAKLSHRNQLQWHFTLLDSPEINAFALPGGYVYV
                     TRGIMAYLQSEAELAGVIGHEI"
BASE COUNT          368 a          575 c          638 g          429 t
ORIGIN      
        1 aacgctgaat tgaactgtat cccctggcca acaaaaactg tagtcggcgt ttaccccgat
       61 cgtgtgtcag aaaaagcctg acacggtgtc agtgccgaca gccgcaatcc tcagagcagc
      121 cgcactttca cgctcttgcc cttgatcttg cccgcgttga gcttttgcat ggcttccagt
      181 gcgatagcac gttgcaccgc cacaaaggtg gtgaattcag tcacgttgat cttgccaatc
      241 tgctcgcggc tgtaaccggc gtctgcggtg agcgccccca gtacgtcacc cgggcggatt
      301 ttctccttgc gaccgcccaa aatttgtagc gtcaccatgg gcggcagcaa gggccccgag
      361 ccggtggggc tgagttcgtc gattttgtgc cagacggagt cgagtttttg atattgctcg
      421 attttgccga ccgcgcccat ctcgtacatg gcagccaggc tcaatgccag gcccgtctcg
      481 cccgcgcgac cggtgcggcc aatgcggtgc acatgcacct cggggtcggg ggtgatgtcc
      541 acattgatca ccgcttccag ttccttgatg tcaatgccgc gtgaggccac gtcggtggcc
      601 accagaatgg acgtgctgcg gttggcaaac tggaccagaa cctggtcacg ttcacgctgc
      661 tctagctcac catacaaggc cagcgcgcta aagccctgct gctgcaggta agtcaccagg
      721 tctttgcatt gctgcttggt gttgcaaaag gccagcgtgc tgaccgggcg gaaatggttc
      781 agcagacggg ccaccacgtc aaagcgggtc gggcgggtga cttcataaaa gcgctgctca
      841 atcaggcttt gggtggctgg tggcgctacg gtgatctgtt caggcgaacg catgaattgt
      901 ttggccagct tttcaatgcc ttcggggtag gtggccgaaa acagcagggt ttgccgctcg
      961 ggggggcatt gtttggtgac cgtggcaatg tcgtcaaaaa agcccatgtc cagcatacgg
     1021 tcggcctcat caagtaccag ggtattgagg gcgtccagcg ccaagtaacc ccgcgccagg
     1081 tggtccatta cccgtccggg ggtgccgacc acaatgtgtg cgccgttttc caggctggca
     1141 cgctggccgc gcagggccac gccgccgcac agggtgacta ccttgacgtt gtccatggcg
     1201 cgggccaggc gacggatttc gactgtgacc tggtcggcca gttcgcgcgt ggggcacagc
     1261 accagcgcct ggacggcaaa gcgccgtggg ttcaggcgct ccagcagcgc caggccaaag
     1321 gcggcggttt tgccgctgcc ggtttgcgcc tgggcgatca ggtctttgcc ggccagcgcg
     1381 gcaggcaggc tgccggcttg aatgggtgtc atgctcaggt agcccagttg ctccaggttg
     1441 gcaagtgtgg ccgggttcag gggcagggtg ttgaacggga ttttttcact gtttgtcatg
     1501 ctttgattat cggtacagcg ccgggccgtg cagttgcgcc ggcatcttgg gcaaaatagc
     1561 gccgcgtcgg cagcgctgcc gttcactccc cgttgcgtgt ttgacatcat ttgcaaaaga
     1621 gactctatgc gatttaaatt gcttcccctc gtgcttgccg ccctgctgac cgcctgcggc
     1681 agtgccgtgg tcaacccggt gtcgggtcag gccgaacgct cggtgatgag cgaacagact
     1741 gaagtggcgg agggggccaa ggcgcacaag gaggtgttgc aagagtatgg cgtggtgaaa
     1801 gatgcccggc tgcaagccta tgtcaacgaa cttggccagc gactggccaa gctgtcgcac
     1861 cgcaaccagc tgcaatggca tttcaccctg ctggacagcc ccgagatcaa tgctttcgcg
     1921 ctgcccggtg gttatgtgta cgtgacacgc ggcatcatgg cgtatttgca aagtgaggcc
     1981 gaactggccg gcgtgattgg acatgaaatt
//
LOCUS       DAOSLL010000703         2005 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_48650_length_2005_cov_3.789744, whole genome shotgun sequence.
ACCESSION   DAOSLL010000703 DAOSLL010000000
VERSION     DAOSLL010000703.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48650_length_2005_cov_3.789744"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            69..1547
                     /locus_tag="PLB25_21155"
     CDS             69..1547
                     /locus_tag="PLB25_21155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohemerythrin"
                     /protein_id="HOE44115.1"
                     /translation="MTFKWDSYFETGVEIIDQQHQGLVALINECAPFLTDTEQPDFAR
                     MDLLLDQLFAYAAMHFKTEEGLMVSHGVDPRYYKHHGAAHTLFVEQLTDMRAEVASQS
                     VSLAGPNLLKFLTSWLTIHILDEDQRLARHLDLLASGASAVDAFQTVSAKVRATDPAR
                     AALESALIELYTLLGERNKVLQTVNQALEQSRNQLEQRVQERTQELSAALEQTKAAQA
                     QLLQSEKMAAIGQLAAGVAHEINNPIGFVNSNLGTLKGYVHQLLSVIDAYEGLDSEVS
                     PLHPARQRIERAIADADLIFLRKDISDLLDESMSGLGRVKKIVQDLKDFSHVDQSDWQ
                     DVDLNVGLESTLNVIANEIKYKARVNKTFGLLPQLRCVPAQLNQVFMNLLINAAQAME
                     GKQEMGRIDVATGADDDAVWVEIRDNGGGMPDDVRRRIFEPFYTTKPVGKGTGLGLSI
                     SYDIVTKKHGGRIEVESVTGAGSTFRVVLPRQPNESVLMVQG"
     gene            1553..>2005
                     /locus_tag="PLB25_21160"
     CDS             1553..>2005
                     /locus_tag="PLB25_21160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HOE44116.1"
                     /translation="MPELYELVDEVALFHGLVDHCSLGLIWIDASAQIQFVNPVALRL
                     CACAEGELLGKRIDSLFATALSPDDQLALQRCVEDGRAWSVEAQINRFDGTRFWGHLQ
                     WIPVSQVKAGTAGFCVCLRDFSMPRQEAERMERLASLDHLTQLPNRAQF"
BASE COUNT          457 a          525 c          577 g          446 t
ORIGIN      
        1 gaatacgcag cactaacgga ttggacaaat tccgccggtt tgggcgcgga accccaagag
       61 aaacagccat gactttcaag tgggacagtt attttgaaac cggggtcgag atcatcgacc
      121 agcagcatca ggggctggtc gccttgatca atgagtgtgc gccgtttctg accgacaccg
      181 aacagccgga ctttgcgcgc atggacctct tgctcgatca gttgtttgcg tatgccgcca
      241 tgcatttcaa aaccgaggaa ggcctgatgg tctcgcacgg tgtggacccg cgctattaca
      301 aacatcatgg cgcagcgcac acgctgtttg ttgagcaact gaccgacatg cgtgctgaag
      361 tggcatcaca aagcgtatct ttagcggggc caaatttgct caagttcctg accagttggc
      421 tcacgattca catacttgat gaagaccagc gtttggccag gcacctggat ctgctagcca
      481 gcggcgcttc cgctgtcgat gcattccaaa ccgtctctgc gaaggtacgc gctactgatc
      541 cggcgcgggc tgcgctggag tcggccttga ttgagttata caccttgttg ggtgagcgaa
      601 acaaggtctt gcagacggtc aaccaggcac tggaacagtc gcgcaatcag ctggaacaac
      661 gggtacagga gcgcacgcaa gaactgtcgg cagcgcttga gcaaaccaag gccgctcagg
      721 cccagttact gcagtccgaa aagatggcgg cgataggcca actcgccgca ggcgtggccc
      781 acgaaatcaa caacccgatt ggctttgtca actccaacct gggcacactc aaaggctacg
      841 tccatcaact gctcagtgtg attgacgctt atgaagggct ggattctgaa gtgtcgccgc
      901 tgcatccagc gcggcagcgg attgaacgtg ccattgctga cgcagatttg atttttctgc
      961 gcaaagacat ttccgatctg ctcgacgaat ccatgagcgg cctgggccgg gtcaaaaaaa
     1021 ttgtgcaaga cctgaaggat ttttctcacg tggaccaatc cgattggcaa gatgttgacc
     1081 tgaatgtcgg actggaatcc acgttgaacg tgatcgccaa cgagatcaaa tacaaggcgc
     1141 gagtgaacaa aacatttggt ctgctgccac agttgcgctg cgtaccagca caactcaatc
     1201 aggtcttcat gaatctcctg atcaacgccg cgcaggccat ggaaggcaaa caggaaatgg
     1261 gccgaatcga tgtggcaaca ggtgctgatg atgatgccgt gtgggttgaa atccgggaca
     1321 acggaggggg catgcccgac gatgtaagac ggcgaatatt cgaaccgttc tacaccacca
     1381 agccggtcgg caagggcacg ggcctggggc tctccatttc ctacgacatt gtgacaaaaa
     1441 aacatggtgg gcgcattgag gttgaaagcg tgacgggagc aggcagcacg tttcgggttg
     1501 ttcttccgcg ccagccaaat gaatcggtgt tgatggtgca ggggtgatca agatgcctga
     1561 actttatgag ctggtcgatg aagtggccct gtttcatggc ctggttgacc attgctcgct
     1621 cggtttgatc tggattgacg ccagcgctca gattcaattc gtgaaccccg tggcgcttcg
     1681 cctttgtgct tgtgccgaag gcgaattgct tggcaaaagg attgacagcc tttttgccac
     1741 cgccctttcg cccgatgatc aattggccct gcagcgttgt gttgaagacg gtcgggcctg
     1801 gtcggtcgag gcccaaatca atcgatttga cggcacgcgt ttttgggggc atctccaatg
     1861 gatccctgtt tcccaggtga aggccggtac ggcaggtttt tgcgtctgtt tgcgtgactt
     1921 ctccatgccc cggcaggagg ccgagcgaat ggaaagactt gccagtcttg atcatttgac
     1981 tcagctgccc aatcgcgccc agttt
//
LOCUS       DAOSLL010000704         2004 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_48679_length_2004_cov_3.877886, whole genome shotgun sequence.
ACCESSION   DAOSLL010000704 DAOSLL010000000
VERSION     DAOSLL010000704.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48679_length_2004_cov_3.877886"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1456
                     /locus_tag="PLB25_21165"
     CDS             <1..1456
                     /locus_tag="PLB25_21165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HOE44117.1"
                     /translation="TKDGFWRGQVWFKKVDHAGFAVEASWVLLRDESGAVVSTIAFFR
                     DLTDELAAQERIEQLAYSDALTGLPNRLMLSQRVAFALHLAERNGSEFGVFFLDLDRF
                     KNINDSMGHAFGDRVLIDVAGRLKRCLRDVDTLCRIGGDEFVIFLQELDAHGAEILAQ
                     RILDVMGQPFQIDDMNFSIGCSLGIALYPEDGRTLDELIKCADSAMYQVKSRGRGSFR
                     FYQPQMNVDLLSHLKMDHAMRQAMEHGLFRLHYQPQISLATGQLLGAEALIRWFDAEL
                     GNVSPGLFIPLAEESGFIVVIGNWVLEEAVRQACIWQNCGTPVSVSINVSALQFQQTN
                     FVEIVASVINSAGLAPQLLELELTESILVKDANETLGRLHALAALGVAMSIDDFGTGY
                     SSLAYLKKFPISKLKIDQSFVRGLPDDESDRAIVSATIGMARGLKLKVIAEGVETVAQ
                     RDYLASLKCEAFQGYLCSPGLPATEFERLMASLK"
     gene            complement(1480..>2004)
                     /locus_tag="PLB25_21170"
     CDS             complement(1480..>2004)
                     /locus_tag="PLB25_21170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018906422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HOE44118.1"
                     /translation="DLQGRTDVAGLYAIGETACTGLHGANRLASNSLVECMVFAQATT
                     NHIAQAQNGASAHLPSWDDSRVIDADETVVISHNWDELRRFMWDYVGIVRTNKRLERA
                     AHRIALLQDEIQEFYANFQVTRDLLELRNLVQVADLIVRSAQARLESRGLHFSRDYPD
                     LLPIALPTILSPNA"
BASE COUNT          408 a          543 c          578 g          475 t
ORIGIN      
        1 cactaaagac ggcttttggc gcggccaggt ctggttcaaa aaagtcgacc acgccgggtt
       61 tgcggtagag gcatcctggg tattgttgcg cgacgagtcc ggtgcggttg tgagtacgat
      121 tgctttcttt cgcgatttga cagatgaact cgcggcacag gagcgcattg aacaactcgc
      181 ttacagtgat gccctgaccg gtttacccaa ccgtttgatg ctgtcacaac gtgttgcgtt
      241 tgcactgcac ttggctgagc gcaatggcag cgagtttggt gtcttttttc ttgatctgga
      301 ccgtttcaag aacatcaatg actcgatggg ccatgccttc ggagaccgtg tattgattga
      361 tgtggccgga cggctcaagc gctgtctgcg tgatgtcgat accctgtgcc gtatcggtgg
      421 cgacgagttt gtgatcttct tgcaagagct tgatgctcat ggcgctgaaa tcctggcgca
      481 gcgcattctt gatgtcatgg gtcagccgtt tcaaatcgac gacatgaatt tttccatcgg
      541 ctgcagcctg ggcattgcgc tgtacccgga agacggcaga acgcttgacg aactgatcaa
      601 atgcgctgat tctgcgatgt accaggtcaa gtcgcgcggg cgtggcagtt tccgcttcta
      661 tcagccacag atgaatgtcg atctgctctc gcatctcaag atggaccacg ccatgcgcca
      721 ggccatggag cacggtctgt tccgcttgca ttaccaaccg caaatctctc ttgcaaccgg
      781 gcagttgctg ggggcagagg cactgattcg ctggtttgat gccgaactcg gcaacgtctc
      841 accggggctg tttattcccc tggccgaaga gtcgggcttc atcgttgtga ttggcaattg
      901 ggtgctggag gaagccgttc gacaggcctg catctggcaa aactgtggca cgccggtgag
      961 cgtctcgatc aacgtatcag cgctgcagtt tcaacaaacc aattttgtcg aaatcgttgc
     1021 gtctgtgatc aacagtgccg gcctggctcc gcagcttctg gagcttgagt tgaccgaatc
     1081 catcctggtc aaagatgcca acgaaaccct cggccgactg catgccctgg cagcgctggg
     1141 ggtcgcaatg tcgattgatg actttggcac cggttactca agtctggcgt acctgaaaaa
     1201 gttccctatt tccaaactca agattgacca gtcgtttgtc cggggcttgc cagacgacga
     1261 aagtgaccgt gcaattgtca gcgccaccat tggtatggca cgcggactca aactcaaagt
     1321 gattgctgaa ggtgtggaga ctgtggcgca acgagactac ctggccagct tgaaatgcga
     1381 agcttttcag ggctatctgt gttcaccggg actgcctgcg accgagtttg aacgactgat
     1441 ggcgtcgctg aagtaagcac gctgcggctg ggtgccgatt caggcgttgg ggctcaagat
     1501 ggtcggcagg gcaattggca gcaggtcggg ataatcccgg ctgaaatgca agcctcggct
     1561 ctcaaggcga gcctgtgcgc ttctgacaat caaatccgcc acctgcacca gattgcgcaa
     1621 ttccagcagg tcgcgcgtga cctgaaaatt ggcataaaac tcttgaattt cgtcttgcag
     1681 caaggcaatc cggtgtgccg cccgttccag ccgcttgttg gttcgcacaa tgccgacgta
     1741 gtcccacatg aagcggcgca gttcgtccca gttgtgcgag atcaccacgg tctcgtcagc
     1801 atcaatcacc cggctgtcgt cccagctcgg caaatgcgct gaagcaccat tttgggcttg
     1861 cgcgatgtgg ttggtggtag cctgtgcaaa caccatgcat tccaccagcg aattgctggc
     1921 cagccggttc gcgccgtgca gcccggtaca ggcggtttct ccaatcgcgt acagcccggc
     1981 gacatcggta cgaccctgca agtc
//
LOCUS       DAOSLL010000705         2003 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_48708_length_2003_cov_5.038501, whole genome shotgun sequence.
ACCESSION   DAOSLL010000705 DAOSLL010000000
VERSION     DAOSLL010000705.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48708_length_2003_cov_5.038501"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            2..1264
                     /gene="hemN"
                     /locus_tag="PLB25_21175"
     CDS             2..1264
                     /gene="hemN"
                     /locus_tag="PLB25_21175"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020228283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HOE44119.1"
                     /translation="MEQRHGGAAAMVLPLSMYVHIPFCESLCYYCACNKIITRHHDRA
                     TAYLRYLEVEVNLHTQHLGFGQVVSQLHLGGGSPTFLSDIELRELMGMLRRNFSLSPG
                     GEFSIEIDPRTIDAARLDVLAELGFNRLSFGVQDFDPAVQKAVHRIQPADQVFALLEA
                     ARQRGFESINVDLIYGLPGQTPESFDRTLVQINALKPDRIALYAYAHLPERFKPQRRI
                     STVDIPTAASKVSMLARSMAAFMAAGYVYIGMDHFALPDDSLAIAKRQGRLHRNFQGY
                     STQPDCDMIGLGVSSIGRVGATYSQNAKTLEEYYDYLNQGQFPVVRGLALTRDDLVRR
                     GVIMALMCQGQLSFESIELGYLINFKQYFATEMKILAGQVEQGLVELDATGIQVTTKG
                     WFFVRAVAMVFDRYLQADRTRAKFSKIL"
     gene            1274..1951
                     /locus_tag="PLB25_21180"
     CDS             1274..1951
                     /locus_tag="PLB25_21180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HOE44120.1"
                     /translation="MQTTLATTALLMGLAGGPHCIAMCGAACAGIGHAAGERGTAAMW
                     LFQVGRVLGYSALGALAAVTMQGLGWLTVQSAALRPVWTMFHAAVMVLGFVLIWKAQQ
                     PIWLEQAGRKMWSVARSLAVGGGTAGPLILGALWTFLPCGLLYSALLVAALTGHAIDG
                     AFVMALFALGTSVSMMLGPWLWLRFGSNSNGDWGVRLAGAALAGSAAWALWMAYAHND
                     APWCTPI"
BASE COUNT          367 a          509 c          611 g          516 t
ORIGIN      
        1 gctggagcag cgacatggcg gagcggctgc catggtgctg ccactttcca tgtacgtgca
       61 tattccgttt tgtgagtcgc tttgttatta ctgcgcatgc aacaagatca ttaccaggca
      121 tcatgatcgt gctacagcat atctgcgcta tcttgaagtt gaagtcaacc tgcacacgca
      181 acacctgggt tttgggcaag ttgtttcgca gcttcaccta ggaggtggtt caccgacctt
      241 tttgtcggac attgaattgc gtgaactcat ggggatgctg cggcgtaatt tcagcttgag
      301 tccgggcggc gagttctcga ttgagatcga ccctcgcacc atcgatgcag cgcggcttga
      361 cgtgctggcc gagttgggct ttaaccgcct gagctttggg gtccaggatt ttgaccccgc
      421 cgtgcaaaag gcagtgcacc gtatccagcc cgctgatcag gtttttgcct tgcttgaggc
      481 agctcgccag cgtggctttg agtcgatcaa cgttgacctg atctatggtt tgccaggaca
      541 gacaccggag tcctttgacc gcactttggt gcaaatcaat gcgctcaagc cggatcgtat
      601 tgctctgtac gcgtatgcgc atttaccaga gcgcttcaag ccccaaagaa gaatatcgac
      661 tgtcgatatt ccaaccgctg catccaaggt ttcgatgctt gcacgttcga tggctgcctt
      721 catggccgct ggctatgtgt acattggaat ggatcatttc gctctgcccg atgattcctt
      781 ggccattgcc aagcgtcagg gccgcttgca ccgcaatttc cagggttaca gcacacagcc
      841 tgattgcgac atgattgggt taggggtttc gtccatcggg cgggttggag cgacctacag
      901 tcaaaacgct aaaacgctcg aagagtacta cgactatctc aaccagggcc aattccctgt
      961 ggtacgcggc ttggcattga cgcgtgatga cctggtacgg cggggagtca tcatggcctt
     1021 gatgtgccag ggacaattgt cctttgagtc aattgaactg ggctacttga tcaacttcaa
     1081 gcagtatttt gcgacggaaa tgaagatcct ggctgggcag gttgagcagg ggctggttga
     1141 attggatgca acgggcattc aggtgaccac caagggctgg ttctttgtcc gcgccgtcgc
     1201 gatggtgttt gaccgctatt tgcaggccga ccgtacccgc gccaagtttt cgaaaatact
     1261 ttaaattcag gtgatgcaaa ccacactcgc tacaaccgcc ttgctgatgg gcctggccgg
     1321 tggcccccat tgcattgcca tgtgtggcgc cgcttgtgcg ggcatcggac atgctgccgg
     1381 cgaacgagga acggcagcga tgtggctgtt tcaggttggg cgtgtgcttg gctattcggc
     1441 gctaggcgca ctggcggcag tgacgatgca gggtttgggt tggctaactg tgcagtcggc
     1501 cgcattgagg ccggtctgga cgatgttcca tgccgcagtg atggtgctgg gttttgtgct
     1561 tatttggaaa gcgcagcagc cgatctggct ggagcaagct ggcagaaaga tgtggtcggt
     1621 tgcgcgcagt ctggctgttg gaggtggcac ggctggccct ctgattctgg gtgctctatg
     1681 gacgtttctg ccctgtggtt tgctttattc tgccttgttg gttgccgctt tgactggcca
     1741 tgcaatcgat ggcgcgtttg tcatggcact ttttgccttg ggcaccagcg tgtccatgat
     1801 gctgggtccc tggctttggc tgcgctttgg cagcaacagc aatggcgact ggggtgttcg
     1861 gctggccggt gcggccctgg ctggcagtgc tgcttgggct ttgtggatgg cctacgcgca
     1921 caacgacgcg ccgtggtgca caccaatctg aataaaacag gtaatccccc ggctttgccg
     1981 gggcgacagt aggggtttta cat
//
LOCUS       DAOSLL010000706         1967 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_49947_length_1967_cov_6.738494, whole genome shotgun sequence.
ACCESSION   DAOSLL010000706 DAOSLL010000000
VERSION     DAOSLL010000706.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49947_length_1967_cov_6.738494"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1758)
                     /locus_tag="PLB25_21185"
     CDS             complement(<1..1758)
                     /locus_tag="PLB25_21185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44121.1"
                     /translation="MPQGTIRIRGARQHNLKNLDLDIRTGELTVVTGPSGSGKSSLVF
                     DTLFAEGQRRYVETFSAYARQFLDRMDKPAVDKVEGVPPAIAIDQTNPVRSSRSTVGT
                     MTELNDHLKLLFARAGQLFDRDTAQAVRHDTPETIFADIQARAAALAALGSLDQVQEL
                     ADVRLAITFPVELPASASADEIELWLSVSGFTKVQAQREVDTAADAGSKAELAGQAFD
                     KTKAAARSKTAAKAVATSTGTRRKVLDVVADRFKLGSTERARVLEAIEVALKHGSGHL
                     SVYLLNRHPDQAGQALVATLSIANEVPATALNSNLQPDAPDAAVAVPEIWRYSTGLHC
                     PQSNLRYADPMASMFSFNSAVGACDTCRGFGRVIGVDYGLVIPNDKLTLRAGAIKPIQ
                     TPAWQEAQDDLMRHAEASGIPRDTPWYKLTPEQQQWVINGSPQWNGKWNQHWFGIKRF
                     FEYLETKSYKMHIRVLLSKYRSYTPCPDCGGARLKTESLLWRIGSKAQADAVLPPAGR
                     FLPSGVKWTRPQLEALPGLCLHDLMLLPIDRLRRFFDSLAAGTSRAEPALAVIHAQPA
                     DGSESGPGFGPAHASLATQV"
BASE COUNT          354 a          583 c          624 g          406 t
ORIGIN      
        1 cacttgggta gccagactgg cgtgcgctgg cccgaaaccc ggccccgact ctgacccgtc
       61 ggcaggctgt gcgtggatga ctgccaacgc tggttcggcg cggctcgtgc cagcggccag
      121 gctgtcaaaa aagcgcctga gccggtcaat tggcagcagc atcaaatcat gcaggcacaa
      181 cccgggcagc gcttcaagct gggggcgagt ccatttcacc ccgcttggca aaaagcggcc
      241 ggctggcggc agcaccgcgt ccgcctgcgc cttgctgccg atgcgccaca gcaggctttc
      301 ggttttcagg cgggcaccgc cgcaatccgg gcagggcgtg tagctgcggt atttggacag
      361 cagcacgcgg atgtgcatct tgtacgactt ggtctcgagg tactcaaaaa agcgcttgat
      421 gccaaaccag tgctggttcc acttgccatt ccactgcggc gagccgttga tcacccactg
      481 ctgctgctcg ggtgtgagct tgtaccaggg tgtgtcgcgc gggatgcccg aggcctcggc
      541 gtggcgcatc aggtcgtctt gggcctcttg ccaggccggg gtctggatcg gcttgatggc
      601 cccggcgcgc agcgtcagct tgtcattggg aatgaccagg ccgtagtcca ccccgatcac
      661 ccggccaaaa ccgcggcagg tgtcgcaggc accgaccgcc gagttgaagg aaaacatcga
      721 tgccatcggg tcggcgtagc gcaggttgct ctgagggcag tgcaggccgg tggagtagcg
      781 ccagatttca ggcactgcca ccgctgcgtc aggcgcgtct ggctgcaagt tgctgttcag
      841 ggccgtggcg ggcacctcat tagctattga taatgtagct accagcgctt gtcctgcttg
      901 gtctggatgc ctatttagca gataaacgct gagatgacca ctgccgtgct tgagggcaac
      961 ttcgatggct tcgagcacgc gagcgcgctc ggtactgccg agcttgaaac gatcagccac
     1021 gacatccagc accttgcgcc ttgtgccggt cgaggtggcc accgccttgg cggcggtttt
     1081 cgatctggcg gctgctttgg ttttatcaaa ggcttgacct gccagctcgg cctttgaacc
     1141 agcatcggct gcggtatcga cttcgcgttg tgcctgcacc ttggtaaaac cgctcaccga
     1201 cagccatagc tcgatttcat cagcgctggc gctcgccggt aattccaccg gaaacgtgat
     1261 ggccaggcga acgtcggcca gttcctgcac ctgatcaaga ctgccaagcg cagccaaggc
     1321 ggcagcgcgc gcctgaatgt cggcaaaaat cgtctcgggc gtgtcgtggc gcactgcttg
     1381 cgcggtgtcc cggtcaaaca actggccggc ccgggcaaac aacaatttca ggtggtcgtt
     1441 cagctcggtc atcgtgccga ccgtggagcg gctggagcgc accgggttgg tctggtcaat
     1501 ggcgatggcc gggggcacac cttcgacctt gtcaacggct ggcttgtcca tgcggtcgag
     1561 aaactggcgc gcgtaggcgc tgaaggtttc aacatagcgc cgctggcctt cggcaaacag
     1621 ggtgtcaaac accaggctgg acttgcccga gccgctcggg ccggtgacaa cggtcagttc
     1681 accggtgcgg atgtcgaggt caaggttttt gaggttgtgc tgacgtgctc cgcgaatgcg
     1741 gatggttccc tggggcatgg atgaagcttt ccgggtggtg gggcgaaaaa tatcgcaagt
     1801 cattttaaga agctggcaat ggcatcgcgc cggatggcct ttttgagtcc ttcgccaact
     1861 gccattctca agccacgcct aagctgaagt tccttcccca aaaagctgag cgccccaatg
     1921 aaacccacgt taattttggt tccgcgttct gtctacggtt tgatctg
//
LOCUS       DAOSLL010000707         1965 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_50017_length_1965_cov_4.844503, whole genome shotgun sequence.
ACCESSION   DAOSLL010000707 DAOSLL010000000
VERSION     DAOSLL010000707.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50017_length_1965_cov_4.844503"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="PLB25_21190"
     CDS             <1..910
                     /locus_tag="PLB25_21190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011485329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HOE44122.1"
                     /translation="VQPQPLQHQGDGTLALADIEAAIKPDDAHYAKTRLLALENTFGG
                     KLLPFAYVQQATQLAARHGLGRHLDGARLFNAAVAQALQVTQENRNNQTPAQYQKALQ
                     ATNNIALAVRDGATDSRHTPNHTIHAATPPGLADIHAQARRIAQCFDSVSVCLSKGLG
                     APVGSVLCGSAKLIQRAHRIRKMAGGGMRQAGILAAAGSYALDHHVARLADDHALAAR
                     LAQGLQGLPGVLVEPAQTNMVFVDLQGAAKTRAGDLLPYLKAHGVLATGLYRLRFVTH
                     LDVDAAGIDHAIAVLRAFFDGSGALA"
     gene            947..1615
                     /locus_tag="PLB25_21195"
     CDS             947..1615
                     /locus_tag="PLB25_21195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HOE44123.1"
                     /translation="MFHGIDNLGLFVAAGIVLNLTPGPDVLFIVTQALKSGWRTGAVA
                     ALGITAGCFVHIVAAALGVSALMAASGTAFAVLKWLGAAYLVYVGWSMLGLGKRQNAT
                     KSIAASAVSTGAAGINHYRSVLIRGFWTNALNPKVALFFLAFLPQFIAPAAEHKTLAF
                     LLLGLVFNANGLWVNLGYAALAAALSQRFARLQQGLGWLQRVAGVLFIGFGLKLALTD
                     NPAN"
     gene            1736..>1965
                     /gene="trpD"
                     /locus_tag="PLB25_21200"
     CDS             1736..>1965
                     /gene="trpD"
                     /locus_tag="PLB25_21200"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465869.1"
                     /note="Catalyzes the conversion of
                     N-(5-phospho-D-ribosyl)-anthranilate and diphosphate to
                     anthranilate and 5-phospho-alpha-D-ribose 1-diphosphate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HOE44124.1"
                     /translation="MSNTYQITPQQALLRTIEHREIFHDEMLHVVRQIMSGEWSPVMI
                     AALITGLRVKKETIGEITAAAQVMREFSTKVQV"
BASE COUNT          373 a          594 c          626 g          372 t
ORIGIN      
        1 cgtgcagccg caaccgctgc agcatcaagg cgatggcacg ctggcgctgg ccgatattga
       61 agcggccatc aagcccgatg acgcgcacta cgccaagacc cgactgctgg cgctggaaaa
      121 cacctttggc ggcaagctgc tgccgtttgc ctatgtgcag caggccacgc aattggcggc
      181 caggcacggc ctgggtcggc acctggatgg tgcgcggctg ttcaatgcgg cggtggcgca
      241 ggcactgcag gtgactcaag aaaataggaa caatcagact ccagcccaat accagaaagc
      301 gctgcaagct acaaataaca tagcattggc agtgcgtgac ggcgcgactg atagccgcca
      361 cacccctaac cacacgattc acgcggcaac gccgcccggt ctggccgaca ttcatgccca
      421 ggcgcggcgc atcgcccagt gttttgacag cgtctcggtg tgcctgagca agggcctggg
      481 ggcgccggtt ggctcggtgt tgtgcggcag cgcaaaactg atccaacgcg cgcaccgcat
      541 tcgcaaaatg gccggtggcg gcatgcgcca ggccggcatt ctggcggcgg cgggcagcta
      601 tgcgctggat catcatgtgg cgcggctggc tgacgaccat gcgctggcgg cccggctggc
      661 ccaaggttta cagggtttgc ccggcgtgtt ggtggagcca gcccagacca atatggtgtt
      721 tgttgatctg caaggtgcgg ccaaaacccg cgctggcgac ctgttgccct acctgaaagc
      781 ccacggcgtg ctggccacgg gcctgtaccg gctgcgtttt gtcacccatc tggacgtgga
      841 tgcggcgggc atcgaccatg ccatcgcggt gctgcgggca ttttttgacg gcagcggcgc
      901 gctggcctga gccccggcga cttcaggcca caccagggct cacctcatgt ttcatggcat
      961 cgacaacctg ggtttgtttg tggcagcagg cattgtgctg aacctgaccc cgggccccga
     1021 tgtgctgttc atcgtgaccc aggcgctcaa gagcggctgg cgcacgggcg cggtggcggc
     1081 gctgggcatc acggcgggtt gttttgtgca catcgtcgca gccgccctgg gcgtgagcgc
     1141 gctgatggcc gcatcgggca ccgcgttcgc cgtcctgaaa tggctgggtg cggcttacct
     1201 ggtgtatgta ggctggtcca tgctcggcct cggcaaacgc cagaatgcta caaagtcaat
     1261 agctgccagc gcagtaagca cgggggccgc aggcataaat cactatagaa gcgtgctcat
     1321 cagagggttt tggacgaatg cgctcaaccc caaggtggcc ttgttctttc tggcattttt
     1381 gccgcagttc attgcgccag cagccgagca caagacgctg gcatttttgt tgctcggcct
     1441 ggtgttcaat gccaatggcc tgtgggtcaa cctgggctat gcagcgctgg cggcagcgct
     1501 gtcgcagcgc tttgccaggc tgcagcaggg cctgggctgg ctgcagcggg tcgcgggtgt
     1561 cttgttcatc ggttttggcc tgaagctggc gctgaccgac aatccggcca actgaccgtc
     1621 gcagcggtca cggatgcgaa ccggcccggc acccaaaccg tgcagcgaaa aaaacaattc
     1681 gctcaaaata cgccctgcga caatgccttt atccactttt tcagccagcc aagacatgag
     1741 caatacctac cagatcaccc cgcaacaagc gctgttgcgc accatcgagc accgcgaaat
     1801 tttccacgac gaaatgctgc atgtggtgcg ccagatcatg agcggcgaat ggagtccggt
     1861 gatgattgcc gcgctgatca ccggcttgcg tgtcaaaaag gaaaccatcg gcgagatcac
     1921 cgctgcggcg caggtgatgc gcgagttttc caccaaggtg caggt
//
LOCUS       DAOSLL010000708         1963 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_50102_length_1963_cov_3.536164, whole genome shotgun sequence.
ACCESSION   DAOSLL010000708 DAOSLL010000000
VERSION     DAOSLL010000708.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50102_length_1963_cov_3.536164"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            198..>1963
                     /gene="metG"
                     /locus_tag="PLB25_21205"
     CDS             198..>1963
                     /gene="metG"
                     /locus_tag="PLB25_21205"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019426677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HOE44125.1"
                     /translation="MPRQLFVTTALPYANGNFHIGHIMEYIQADIWVRFQKMMGNAVD
                     FVCADDTHGAPIMIAAEKAGVTPQQFVADIAAGRKPYLDGFHIGFDNWHSTDGPENHE
                     LARQIYRDLRDNPDGSLIETRTIEQFFDPEKNMFLPDRFIKGECPRCHAKDQYGDNCE
                     NCGAVYAPTDLIKPYSALSGARPELKSSEHFFFKLSDPRCVAFLEHWTQDGKLQPEVA
                     NKIKEWFSVRTNPDGSKSEGLGDWDISRDAPYFGIEIPDAPGKYFYVWLDAPIGYLAS
                     LKNLLGKRGQDYDTYMANPLLEQYHFIGKDIVTFHTLFWPATLHFSGRKTPDNIFVHG
                     FLTVNNGEKMSKSRGTGLDPLKYLSLGMNPEWLRYYLAAKLSARNEDIDFNAEDFMLR
                     VNSDLIGKFVNIASRAAGFLSKRFDGKLGDVPEDGAALLHHLRSERSSIELLYESREF
                     AKALRETMLMADRVNAYVDANKPWDLAKQAGQEARLQGVCTVCIKVFRVLTCYLKPVM
                     PELARQVEAFLKIAPLSFANVSQPLEAGHVIGSYQHLMQRVDVKQLEALFEPPDQTES
                     ALAAIENVVNRQPGGEPIAATI"
BASE COUNT          459 a          590 c          531 g          383 t
ORIGIN      
        1 acaaagccag cgccaatgtg agcgatctca gtgcaagaaa tgccattcca accaactttt
       61 ccttatgcca ttgagctgcg gcgtactgcc acgaagcgcc caaagttgtt gggggcgacg
      121 gtacctcaaa tacacggttc ggtcaacaaa gttgcgcccg actaaaatca aaggctgtcc
      181 ttgaaagccc ctgccccatg ccacgccaac tctttgtcac gaccgccctg ccctatgcca
      241 acgggaattt ccacatcggc cacatcatgg aatacattca ggccgacatc tgggtgcgct
      301 ttcaaaagat gatgggcaat gcagtcgatt ttgtctgcgc cgacgacacc catggcgcgc
      361 cgatcatgat tgccgcagaa aaagccggtg tcacgccgca gcagtttgtt gctgacattg
      421 ccgccgggcg caaaccgtat ctggacggct ttcacatcgg cttcgataac tggcacagca
      481 ctgacgggcc ggaaaatcac gaactggcta ggcagattta ccgcgatctg cgcgacaacc
      541 cggacggctc gctgatcgag acccgcacca ttgagcagtt ttttgacccg gaaaagaaca
      601 tgttcctgcc tgaccgcttc atcaagggcg aatgccccag atgccatgcc aaagaccagt
      661 atggtgacaa ctgcgagaac tgcggtgcgg tctatgcgcc gaccgatctg atcaagccct
      721 attcagcgct gtccggagcc cgccccgaac tgaaaagctc ggaacacttc ttcttcaaac
      781 tgtctgaccc gcgctgtgtg gcgtttcttg agcattggac gcaggacggc aagttgcagc
      841 ctgaagtggc caacaagatc aaggaatggt tcagcgtgcg caccaacccg gacggctcca
      901 aaagcgaagg cctgggcgac tgggacatca gccgcgacgc gccttacttc ggcattgaaa
      961 ttcccgatgc gccgggcaag tacttttacg tctggctgga cgcaccgatt ggctacctgg
     1021 catcgctgaa aaacctgctg ggcaagcgcg gccaggacta tgacacgtac atggccaatc
     1081 cgctgctgga acaataccac ttcatcggca aggacatcgt gacctttcac accctgttct
     1141 ggccggccac gctgcatttc agcggtcgca aaacccccga caacattttt gtgcatggct
     1201 ttctgaccgt caacaacggc gaaaaaatga gcaaaagccg gggcaccggg ctggatccgc
     1261 tcaaatacct gagcctgggg atgaacccgg aatggctgcg ctattacctg gcggccaagc
     1321 tcagtgcgcg caatgaagac attgacttca atgccgaaga cttcatgctg cgcgtcaaca
     1381 gcgacctgat tggcaagttt gtcaacattg ccagccgcgc cgccggtttc ctgtccaagc
     1441 gctttgacgg caaactgggc gacgtacccg aagacggggc cgccctgtta caccatttgc
     1501 gcagtgagcg cagcagcatc gagttgctct acgagagccg cgaatttgcc aaggcgctgc
     1561 gcgaaaccat gctaatggct gaccgtgtca acgcctatgt cgatgccaac aagccctggg
     1621 acctggccaa gcaggccggg caagaagcgc ggctgcaagg ggtttgcacc gtctgcatca
     1681 aggtctttcg ggtgctgacc tgctacctga agccggtcat gccagagctg gcacgccaag
     1741 tcgaggcatt cctgaagatc gcgccgctta gctttgccaa tgtcagccaa ccgcttgagg
     1801 ccggtcatgt gattggcagc taccagcacc tgatgcaacg cgtcgatgtc aagcaacttg
     1861 aagcactttt tgagcctcca gaccagactg agtctgcgct ggcagctatt gaaaatgtag
     1921 taaatcggca gcccggcggc gagcccatag ccgctaccat cag
//
LOCUS       DAOSLL010000709         1958 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_50288_length_1958_cov_5.398318, whole genome shotgun sequence.
ACCESSION   DAOSLL010000709 DAOSLL010000000
VERSION     DAOSLL010000709.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50288_length_1958_cov_5.398318"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="PLB25_21210"
     CDS             complement(<1..948)
                     /locus_tag="PLB25_21210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009552244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HOE44126.1"
                     /translation="MTTQDPQTQPPGKADYRSTLNLPDTPFPMRGDLPKREPLWVKAW
                     EAKGLYQKLRDARAGKPKFILHDGPPYANGQIHMGHAVNKILKDMIVKARQLKGLDAA
                     YIPGWDCHGLPIENAIEKKFGRKLARDDMQAKSRAYATEQIDLQREDFKRLGVLGDWD
                     HPYRTMDFKNEADEIRAFKRVIERGFVYRGLKPVYWCFDCGSSLAEFEIEYADKKSQT
                     LDVGFLSAEPAKLAAAFGLSALAKDAFAVIWTTTAWTIPANQALNLNPALTFALVDTE
                     RGLLVLAETLVEQCLARFGLSGTVVATTLGKNLGGLNFKH"
     gene            complement(1297..1425)
                     /locus_tag="PLB25_21215"
                     /pseudo
     CDS             complement(1297..1425)
                     /locus_tag="PLB25_21215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139446.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1374..>1958)
                     /locus_tag="PLB25_21220"
     CDS             complement(1374..>1958)
                     /locus_tag="PLB25_21220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44127.1"
                     /translation="STLFGLAFWDKSITWDGTVTSEKGTAGLAGTDSRIVAPIDQPPV
                     NFDPVLTNSVGVRFDLAALTSIAYSAKFDASMLVSAGHGMFPASVPPAMRGKGFIAGT
                     CGSNAADNPAPSCTSGTGAAGGNFTDLDIRKIIANRFNTPVWAPYFTAAFGEATANAN
                     RVSTAIATYERTMTFTQSFAPCMSCWVTQKSAPP"
BASE COUNT          394 a          548 c          586 g          430 t
ORIGIN      
        1 gtgcttgaaa ttgagcccgc cgaggttttt acccagtgtg gtggcaacca ccgtgccgct
       61 cagcccaaaa cgagccaggc attgttcaac cagtgtttct gccagcacca gcaagccacg
      121 ctcggtatcg accagcgcaa aagtcaaggc cgggttcaga ttgagcgcct ggttggccgg
      181 aatggtccag gcggtagtgg tccagatgac ggcaaaagca tctttggcga gcgcactcaa
      241 accaaacgct gctgccagtt tggcgggctc ggcagacaaa aaacccacgt caagcgtttg
      301 cgacttcttg tcagcgtatt caatctcgaa ctcggccagg cttgagccgc agtcaaagca
      361 ccagtaaacc ggctttaagc cccgatagac aaaaccacgt tcaatgacgc gcttgaaggc
      421 acgaatctcg tcggcctcgt tcttgaagtc catggtgcga tagggatggt cccagtcgcc
      481 cagcaccccc agccgcttga agtcttcgcg ctgcaggtca atttgctcgg tggcataggc
      541 acggcttttg gcctgcatgt catcgcgtgc cagcttgcgg ccaaacttct tttcaatcgc
      601 gttctcgatc ggcaggccgt ggcagtccca gccggggata taggctgcgt ccagaccctt
      661 gagctggcgc gccttgacga tcatgtcctt cagaatcttg ttgaccgcat ggcccatgtg
      721 gatctggcca ttggcgtagg gtgggccgtc gtgcaggatg aactttggct tgccagcgcg
      781 cgcatcgcgc agcttttggt acaagccctt ggcctcccag gccttcaccc acagcggctc
      841 gcgtttgggc aggtcgccgc gcatcggaaa cggggtgtct ggcaggttca gggtgctgcg
      901 gtagtcggct ttgccgggag gctgcgtttg tgggtcttgt gtggtcatgt tggcgaaagg
      961 gcaaagttgg cgcgggcatc gtggcagtct cggacattgc cagcggtcaa tgcgtgttgg
     1021 gatttgtcat ggcgttcgtc gtgcagtcgg tgtcgctgcc cgggttggcg gattttactg
     1081 taggcacttg cgaacagcat cttcaaacgg cacggcgact gggtcgagcg acgtatgaag
     1141 accctcagcc gaaattcaac ctgctgagct gcccgccaga taaccggcat aggacggcat
     1201 ggtcgcgcat cgatcaatac gccagggctc tatttcaagt caattgttgg tttgggatgg
     1261 ttcagccact gactccaaag cagcgcttgc cgtcaatcaa acagccagtc ccgccaatga
     1321 atccaaaggg ctggccgtgc cacctgctgc tatcttgagc acatgggtgt aaatcatggt
     1381 ggtgctgact tctgtgtgac ccagcaactc atgcacggtg cgaatgactg cgtgaacgtc
     1441 atcgtccgct cgtacgtggc gatagctgtt gaaacacggt tggcgtttgc cgttgcttcc
     1501 ccaaaggccg cagtgaaata gggtgcccag accggggtgt taaaccgatt cgctatgatt
     1561 ttgcggatgt ccagatcggt gaaattgccc ccggcagcac cggtcccact ggtacatgag
     1621 ggtgccggat tgtccgcagc attgctgccg catgttcctg caatgaaacc cttgccacgc
     1681 atggcgggcg ggacagacgc agggaacatg ccatgtccgg cgctaaccag catcgacgca
     1741 tcaaatttgg cgctgtaggc gatggaggtc agtgccgcca aatcgaagcg aacgcccacg
     1801 gaattggtca gcactggatc aaaattgacg ggcggctgat caattggtgc gacgatacgg
     1861 ctatccgtcc ctgccagtcc tgcagtgcct ttttcgctcg tgactgttcc gtcccaggtg
     1921 atcgatttgt cccaaaaggc caaaccaaag agcgtgga
//
LOCUS       DAOSLL010000710         1953 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_50458_length_1953_cov_2.857745, whole genome shotgun sequence.
ACCESSION   DAOSLL010000710 DAOSLL010000000
VERSION     DAOSLL010000710.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1953)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1953
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50458_length_1953_cov_2.857745"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="PLB25_21225"
     CDS             complement(<1..195)
                     /locus_tag="PLB25_21225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44128.1"
                     /translation="MVTTALRKPCRALLLMTNSILGPGVAETTNVIATNNHHVLKLMC
                     GLSGYKRDTNTDFLIRMRKTT"
     gene            101..697
                     /locus_tag="PLB25_21230"
     CDS             101..697
                     /locus_tag="PLB25_21230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011324959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HOE44129.1"
                     /translation="MTFVVSATPGPNMLLVMSSSARHGLRSAVVTMAGCMTALLAMMG
                     LSAAGLGALLHAFPAVFDALRLVGAAYLAYLGIKSWRAPVQDQLPGSTRADEPAAKSG
                     APYRQGFLVAASNPKAIVFAAAFFPQFINPDLAKVPQFVILLATFSVIEVSWYYVYAV
                     SGTKLSAYLQRATVMKAFNRLTGGVFVGFAALMAMARE"
     gene            complement(770..1003)
                     /locus_tag="PLB25_21235"
     CDS             complement(770..1003)
                     /locus_tag="PLB25_21235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44130.1"
                     /translation="MKTQEAAVAMDHKNCGLFKNLQLIRVELSSYVVRPYPTQALAQI
                     ELQDQAPSSFHPRAQHKNPGRGERLILSSEPNP"
     gene            1369..1689
                     /locus_tag="PLB25_21240"
     CDS             1369..1689
                     /locus_tag="PLB25_21240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44131.1"
                     /translation="MIEHLMRPLGILSLMAIANGVFAGIRLRNPSIEPKVALEDTSAV
                     HPSDVETLAAWVQQVSVEAIDGLVKVLSASPVLTRSAAAVVLLGMLMQVAKQRNSAGQ
                     HRVP"
     gene            1702..1935
                     /locus_tag="PLB25_21245"
                     /pseudo
     CDS             1702..1935
                     /locus_tag="PLB25_21245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017879074.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3079 domain-containing protein"
BASE COUNT          431 a          499 c          521 g          502 t
ORIGIN      
        1 tgtggtttta cgcatacgga tcaggaaatc tgtatttgtg tcacgcttgt aacctgaaag
       61 gccgcacatg agcttgagta catggtggtt gttcgtcgca atgacattcg tggtctcagc
      121 cacccccggc cccaatatgc tgttggtcat gagcagtagc gcgcggcatg gtttgcgcag
      181 cgctgtggtc accatggcag gctgcatgac tgccttactg gcgatgatgg gactatcggc
      241 cgctggcctg ggtgctttat tgcatgcgtt tccagcggtt tttgatgcac tacgacttgt
      301 cggggctgcg tacctcgctt acctgggcat caaaagttgg cgagcaccgg ttcaagatca
      361 gctgccggga tcaacgcgcg ctgatgagcc ggccgcaaaa tcaggtgcgc cttacaggca
      421 gggttttctg gtggcagcaa gcaaccccaa ggccattgtg ttcgctgcgg ccttctttcc
      481 ccagttcatc aacccggatc tggccaaggt cccacagttt gtcatcctgc tggccacctt
      541 ctcggtcatt gaggtcagtt ggtactatgt gtatgcagtc tcaggaacaa agctctcagc
      601 atatttgcag cgcgccacag tgatgaaggc gtttaaccgc ctcactggag gtgtatttgt
      661 gggatttgcg gctttgatgg cgatggcacg ggagtgacga gcctgccatg acagccgaat
      721 gattttctgc atagggcagc cacgcgaccg caagccaggg cggtcacgct catggatttg
      781 gttcagaact cagtattaaa cgttcgccac gaccagggtt tttatgctgt gctcttggat
      841 gaaatgacga aggtgcttga tcttgcaact ctatctgagc taaagcttgg gtggggtacg
      901 gtctcaccac atacgaagac aactcaactc ttatcagttg aaggttttta aaaaggccgc
      961 aatttttatg atccattgcg acagcggcct cttgagtttt caagaggtca actccaatga
     1021 aatcaatgaa ctatgcggcg gctgtttgtg attggaaatt gcgtagctgg aacgaccgag
     1081 aacaaagaaa ctcccttttt tgaggtgtgt tgcttcacat tttgaaacac ttcttcgttt
     1141 ccgtatcttg aacttacaca ataaccaggt atccgaactc gatgatccac aattgccacg
     1201 acaagtacgt ttggcaatct ctgttcaact ttttccaggt actcgcccat gaatcgactt
     1261 ccaactcata ccgctccagc gccgtcaggc gttgggcagc cgagcgtttc cgtgcccatc
     1321 cttattggcc aggtctatga atcagcgcca ccgagtctgc gcgcgaggat gattgaacac
     1381 ctgatgcgcc cgctgggcat tctcagtttg atggctattg ccaatggtgt ttttgcaggc
     1441 atccgtttgc gtaacccctc catcgagcca aaggtagcac ttgaagacac ctctgctgtt
     1501 catcctagcg atgtggagac gcttgccgcc tgggtccagc aagtcagtgt cgaggccatc
     1561 gatggactag taaaagtgct ttcagcgtca ccggtactga cccgatctgc agcagcagtt
     1621 gtgctattgg gtatgctgat gcaagtagcc aaacaacgca acagtgccgg tcaacaccgc
     1681 gtcccttaga aaagtaaaag attggccaaa aagttcccca tgcatccagc aatccccgcg
     1741 cgaatctgct gtggctgtga ttagtactgt tcagttgatt caatggcatg tgccaatgag
     1801 cgctcgcccc atcccgttga actttttgga cttgactgga tgacttgggg taccgaacca
     1861 tgggttgggc cgcatgagaa ggagtccgag cggacattct ctggacttgg tggggcacca
     1921 cgaagtacct cgtaacaatt cttggattaa agg
//
LOCUS       DAOSLL010000711         1940 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_50929_length_1940_cov_6.440318, whole genome shotgun sequence.
ACCESSION   DAOSLL010000711 DAOSLL010000000
VERSION     DAOSLL010000711.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50929_length_1940_cov_6.440318"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(476..1903)
                     /locus_tag="PLB25_21250"
                     /pseudo
     CDS             complement(476..1903)
                     /locus_tag="PLB25_21250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464827.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
BASE COUNT          459 a          545 c          520 g          416 t
ORIGIN      
        1 gaaaatctta ctttcaaact ggtggtcggc actgcagcgc aaactgccgg tcacgaacgg
       61 cgggtgcctt tacatttgtg caagccctga tgagcaaacc gtttctgcca tttcttggtt
      121 cttgaaactg gtcttgcgag ggtcttctgg gcaaagagtc gcttctgggc tctgatgggt
      181 accttcgagt cggtggcccg gtcagatttg aagcacactc agatggcaat ttcagaattc
      241 aaatagctgg cattcgtgag tgtcagcaac gggcacgttg tagcccgcca cgaacgtcag
      301 ttgcccggaa ttggaagtgg aaactaaacc ttgagatacc gggcttttgc gaccgaggag
      361 agtcattctg tgctccctca cttccggttt tgtctgaaat ctgtctgccc aaccaccacc
      421 acgggcaact ccgacctcct tgttcgcacc gcccatttca gtcttctgag ccaactcagc
      481 ttcgattttg cgtctcgcgc tccattgcca tctgctcctc caattgttgt cggccttgct
      541 ttgcgactgc gatcagcttg gcactgtcgc ggtaatgcgg gtacatttgc tcaagaagtt
      601 caaggttgtg ctggcgaaac cgctcaatac cttggtcggc tgcgttggac tcaaagccca
      661 atagctccag cacgttgtgc gcactacgca ggctggactc aaatagttcc cgctgaacca
      721 gcatgatgtt tctgtcgcga agcttgttcc agtgcgtcac atcacgcgcc ctggccacaa
      781 tctgtagatg cgcaaagtgg gcttgcacct ggtcaatgat ctgcaaggac tgatcgacat
      841 cgtctacggc caccaccaga atcttcgcag tttctgcccc cgcagtgcgt agcaggtcca
      901 gccgtgtggc atcgccataa aacacacggt agccgaactt gcgcgctgac tcaatcattt
      961 ctgcgtcgtg atcaagaaca gtcgcggcaa tgccgttggc aagcagcacc cgagagacga
     1021 tttgcccata acggccaaag ccggcaatga tgatcgaagc actttgcggt tcggcaatct
     1081 cgtccaattt gggcactccg cagctggcgt agcggggcag tagcaaccga tcgatggcga
     1141 ccaggatcaa ggggctaacc agcatggaga cagccactgc tccgatcagc aaggatgccg
     1201 tttgcgttgt gaacacattg gcaccggcag ccgcttggaa caccacaaag gcaaattcac
     1261 caccctgggt gagcagcaac gtgaaaaccg gccgctcctg gaagggcagc ttcatcattc
     1321 gtgccagtcc aaatatgacg attgccttga cagtcaatag gccaaacaag atcgccagca
     1381 tctggccagg tgaagcgagc aaaaccccaa agtcaatgct catgcccact gcgatgaaga
     1441 acagaccgag gagcaagcct ttgaaaggtt cgaggtcggt ctccagctca cggcggtact
     1501 cgctttcggc cagcagcaca ccagccagaa aggcaccaag cgccatggag agcccgatca
     1561 gttgcatcaa ggcggcgatg ccaaccacca acagcaaaga cgcggcagtg aaaatttccg
     1621 gggttttgga tcgagctatc caacgaaaga tgggtctcaa ggccagtcga ccgccaatca
     1681 cgatgccagc aatcaccgcg aagatggtca atgcctcaat cacctttgct gatgcatcgg
     1741 gcgaggtagt ctggcctgta gccacgccca acaggggtac cagggccaga atcgggatgg
     1801 cggccacgtc ttgaaacaac aaaatggcaa aggaggattg gccgctggtg gtgggcagca
     1861 agttgcgctc attcatgacc tgcagtgcga tggctgtgct agtagttcgt tccatcaata
     1921 cagttactaa ctagttgata
//
LOCUS       DAOSLL010000712         1939 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_50981_length_1939_cov_2.716561, whole genome shotgun sequence.
ACCESSION   DAOSLL010000712 DAOSLL010000000
VERSION     DAOSLL010000712.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50981_length_1939_cov_2.716561"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1339
                     /locus_tag="PLB25_21255"
     CDS             <1..1339
                     /locus_tag="PLB25_21255"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HOE44132.1"
                     /translation="LVVTDTLTGQANRSGLLEYLQAQVQAFADSQIDGFALLLVNLDE
                     FKRINEGMGLPAGDEILKTTTTRLASCIKVGDLVARLSADTFGLVLNGITQGDVAEQV
                     AGRVLQAIRQTYFAESVPIQLKASVGITLFPNDGRDVPILLRQAELAMGSAKSGGGNS
                     CVFFDPVLAESAERRRQLETDMRSALRNQEMVLFYQPVVDLQTHRLAGAEALIRWRHP
                     ARGLVGPDNFVALAEQTGLIVDIGLFVLDAACRQLRDWRQAGLDYTLSFNVSGRQIPD
                     GLPPAKLREMIHHYGIDPAKLALEITEGVMLQDIDKSLAWLQAVHALGLRIHLDDFGT
                     GYSSLSYLKRFPIDTLKIDQSFVKDMQGTGNERTLVGAILAMASSLGLEVVAEGVELP
                     SHRQALRAMGCHYAQGFLFSRPVPAEQFASVAAQLALMLEVVAAEAPVERMLG"
     gene            complement(1370..>1939)
                     /locus_tag="PLB25_21260"
     CDS             complement(1370..>1939)
                     /locus_tag="PLB25_21260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009550865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HOE44133.1"
                     /translation="IIDESANDVLVAVARGDADFGLNFVGSQESDVEFEPLLEESYVA
                     ACHRKHPLADKKQVSWAELSKYDYITAGKGSGNRFLLDQALAGVPDRPQSMYETQHGT
                     TMIGLVEAGLGVAVVPAMAMPQADHPLLVSIPLVDPVVRRKMGLIRRRNAMLTPAAQQ
                     LYDLCLTVQVNSGQQKSVKASQPEPDASP"
BASE COUNT          372 a          567 c          583 g          417 t
ORIGIN      
        1 cctggtcgtt accgacaccc tgacagggca agccaaccgc tccgggctgc tcgaatacct
       61 tcaagcccag gtgcaggcgt ttgccgattc gcaaatcgat ggttttgcgc tgctgctggt
      121 caatctggac gagttcaagc gcatcaacga gggcatgggc ttgcccgccg gtgatgaaat
      181 tctcaaaacc accacgaccc ggctggcaag ctgcatcaag gtgggcgatc tggtggcgcg
      241 cctgtcggca gacaccttcg gcttggtgct caatggcatc acgcaaggtg atgtggcaga
      301 acaggtcgca ggccgggttc tccaggctat tcggcaaact tatttcgctg agagcgtgcc
      361 tatccagctg aaggccagtg tcggcatcac gctgtttccc aatgacggac gggacgtgcc
      421 gatactgctc aggcaggccg agctggccat gggcagtgcc aaatcgggtg gtggcaactc
      481 ttgtgtgttc tttgatccgg ttctggccga atcggccgag cggcgccgac aactcgaaac
      541 cgacatgcgc agcgccttgc gcaatcagga aatggtgctt ttctaccaac cagtcgtcga
      601 tctgcaaacc caccgtctgg caggtgccga ggccctgatc cgctggcggc atccggcacg
      661 cggtctggtg gggccggaca actttgtggc gctggctgag caaaccgggc tcatcgtgga
      721 tattggcctg tttgtgctgg atgcagcctg tcggcagttg cgcgactggc ggcaagcggg
      781 tcttgactac acgctgtctt tcaacgtgtc aggtcggcag attccggatg gcctgccgcc
      841 tgccaaactt cgggagatga ttcaccacta cggcatcgac ccggccaagc tggcgctgga
      901 aatcactgaa ggcgtgatgt tgcaagacat cgacaaatcg ctggcctggc tgcaggccgt
      961 gcacgcgctg ggcctgcgca tccatctgga cgactttggt accggttatt catcgctttc
     1021 ctacttgaag cgtttcccga ttgatacgct gaaaatcgac cagtcctttg tgaaagacat
     1081 gcagggcacg ggcaacgaac gtacgctggt tggtgccatt ctcgccatgg ccagcagtct
     1141 cgggctggag gtggttgccg aaggcgtgga gttgcccagt caccggcagg cgctgcgagc
     1201 catgggttgt cactatgccc agggttttct gttctcgcgc cccgtgccgg cggagcagtt
     1261 tgcgtccgtg gcggcacagc tggccctgat gctggaagtc gtcgctgccg aggcccctgt
     1321 tgagcgcatg cttggctgac agcaggaatt gctgccaaaa aaaccgggct caaggtgacg
     1381 catcgggctc gggttggctg gctttgaccg acttttgttg accggaattg acctgcacgg
     1441 taaggcacag gtcgtacagc tgctgggcag cgggtgtcaa catcgcattg cgtcgccgta
     1501 tcagtcccat tttgcgtcgt accaccgggt cgaccaaagg aatgctgacc aacagcggat
     1561 ggtccgcttg gggcatagcc atggcgggca ctaccgccac acccagaccc gcttccacca
     1621 gaccgatcat cgtcgtgccg tgttgggttt cgtacatgct ttgtggccgg tcaggcacgc
     1681 ccgccagtgc ctgatcaagc aaaaaccggt tgccagaccc tttgccggcg gtaatgtaat
     1741 cgtatttgga taactctgcc caactgactt gcttcttgtc ggcgagcggg tgtttgcggt
     1801 ggcaggccgc cacataactc tcttccagca aaggttcaaa ctcaacatca ctttcctgcg
     1861 atccgacaaa attcaaacca aaatccgcat ccccccgtgc cacggcgaca agaacgtcgt
     1921 tggcgctttc atcaatgat
//
LOCUS       DAOSLL010000713         1936 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_51105_length_1936_cov_2.624668, whole genome shotgun sequence.
ACCESSION   DAOSLL010000713 DAOSLL010000000
VERSION     DAOSLL010000713.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51105_length_1936_cov_2.624668"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            28..588
                     /gene="greB"
                     /locus_tag="PLB25_21265"
     CDS             28..588
                     /gene="greB"
                     /locus_tag="PLB25_21265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010461858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HOE44134.1"
                     /translation="MSKAFTKESDDSEDEGAVLPQLPVNGKNYITPRGYASLRSELLN
                     LIDVERPKIVDVVHWAASNGDRSENGDYLYGKKRLREIDRRIRFLTKRLEIAEVVDPT
                     VHHGNDQIFFGASVSYLDDDGKLRRVTILGIDEAVSANGEVSWVSPIARTLLKSRLGD
                     VLRLPTPAGVVDIEVVSVDYPAPVIR"
     gene            complement(578..>1936)
                     /locus_tag="PLB25_21270"
     CDS             complement(578..>1936)
                     /locus_tag="PLB25_21270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HOE44135.1"
                     /translation="LHQLYKPVPGKFKDHIAISKINGYQSLHTTLVGPSGINVEFQLR
                     TEAMHLVAETGVAAHWLYKDSDSSEVTHGERMGSKWLQSLLDIQDETRDAAEFWDHVK
                     VDLFPDAVYVFTPKSQILAMPRGATVVDFAYAIHSNVGDHTLSAKVNDEQVPLRTEVK
                     NGDVIEIITTPEATPNPAWLGFVRTARARSKIRQHLKTLAQTESESLGEKMLLQALRA
                     EGIENLPAETNQNQAIWDKLLRFSGNKSRAELMTDIGLGKRIASIVAKRLTKLLADTG
                     EKPDALLLTRERFGSGEVVSQGNLSIDGSESTSVRFAHCCRPIPGDKIVGYLGRGEGL
                     TVHSSDCQVAKKLQYKDSERFIGVEWSDEPVRTFETGIVVTVDNGKGVLAKVAGALAA
                     AEADITHIDMAAESSHGVMDLRFVIGVRDLNQLDLVFRNLRRTPAVLKVQRSKNTAQI
                     SE"
BASE COUNT          411 a          534 c          526 g          465 t
ORIGIN      
        1 atttgcgcca atgattgcag atattccatg agcaaagcgt ttaccaaaga gtccgacgat
       61 tcggaggacg agggtgcggt attgccgcaa ttgccggtca acggcaaaaa ctacatcaca
      121 ccgcgcggct acgccagttt gcgttctgag ttgcttaacc tgattgacgt tgaacggccc
      181 aaaattgtcg atgtcgttca ttgggctgcc agcaacggcg accggtcaga gaacggtgac
      241 tacctctatg gcaagaaacg cttgcgtgaa attgatcgac ggattcgatt tctgacaaag
      301 cgtctggaaa ttgccgaagt tgtggaccca acggtgcacc atggcaatga tcagatattt
      361 tttggcgcca gcgtgtctta cctggatgac gatgggaagt tgcgccgtgt caccattttg
      421 gggattgacg aggcggttag cgccaatgga gaggtaagct gggtgtctcc gatcgcaaga
      481 acgctgctga aatcccgctt gggcgatgtc ttgcggctgc caacgcctgc gggtgtggtg
      541 gatattgaag tggtgagcgt ggactacccg gcacctgtca ttcgctgatc tgagcggtgt
      601 tttttgatct ctgaaccttt aacacagcag gtgtccggcg caggttcctg aacaccagat
      661 cgagttggtt gaggtctcgt acaccaatca caaatcgcaa atccatcaca ccatgagacg
      721 actctgcggc catgtcgatg tgggtaatgt ctgcttccgc cgcagccagc gcgcctgcca
      781 ccttggcaag cacccctttg ccgttgtcta ccgtgacgac aatgcccgtt tcaaaagtgc
      841 gaaccggttc atcagaccac tccacgccga taaatcgctc actgtccttg tactgaagtt
      901 tctttgccac ctgacagtcg ctcgaatgca ctgtcaagcc ttcaccacgg cccaggtagc
      961 ccacgatctt gtcgccaggt atgggtctgc agcagtgcgc aaagcgcaca gacgtgctct
     1021 cactgccgtc aattgacaaa ttaccctgcg acaccacctc gccggagccg aatcgctcac
     1081 gtgtgagcag caaggcgtca ggtttttcgc ccgtgtctgc aagcagtttg gtcaggcgtt
     1141 ttgccacaat gctggcgatg cgcttgccaa gacctatgtc ggtcatcagt tcggcccggc
     1201 tcttgttgcc gctaaagcgc agcaatttgt cccagatggc ttgattttga ttggtttcag
     1261 ctggcaggtt ttcaatgcct tcggcgcgca gggcttgcag aagcatcttc tcacccaggc
     1321 tctctgactc ggtttgtgcc agcgtcttga ggtgctgtcg gatttttgat cgtgcacgcg
     1381 ctgtgcggac aaagcccaac cacgctggat tgggcgtcgc ctccggcgtt gtaatgatct
     1441 cgatcacatc gccattcttg acctctgtcc gcaatgggac ctgttcatcg ttgaccttcg
     1501 cagacagcgt atggtctccc acgttgctgt gaatcgcata agcaaaatca acaaccgtgg
     1561 caccacgagg catggccagg atttgacttt tcggagtgaa tacgtaaacc gcgtcgggaa
     1621 acaggtcaac cttgacgtgg tcccaaaact cggcggcgtc acgggtttcg tcttggatat
     1681 cgagcagtga ctgcagccac ttggagccca tgcgttctcc gtgggtgact tcactcgaat
     1741 cagaatcctt gtacagccag tgtgcggcaa cgccagtttc agccaccaga tgcatcgcct
     1801 ccgtgcgaag ctgaaactcc acattgatac ccgacggccc caccagcgtg gtgtgcaacg
     1861 actggtaacc gttgatcttg ctgatggcaa tgtggtcctt gaactttccg ggaaccggct
     1921 tgtacaactg atgcaa
//
LOCUS       DAOSLL010000714         1930 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_51297_length_1930_cov_6.989333, whole genome shotgun sequence.
ACCESSION   DAOSLL010000714 DAOSLL010000000
VERSION     DAOSLL010000714.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51297_length_1930_cov_6.989333"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="PLB25_21275"
     CDS             complement(<1..508)
                     /locus_tag="PLB25_21275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HOE44136.1"
                     /translation="MVTLVDALRQFGPAYLRQHTLSTAQAKAWRAIVACRTAALGGEQ
                     LACDTCGHSHWQYHSCRNRHCPTCGTRAKDAWLQGRLAEALPVPYAHLVFTLPHSLNG
                     LYGAHPRWVIDTLFACTAQTLAEFAANPKWMGVSAGTPAFSLVLHTWGQDLQRHIHLH
                     AVMACGVLA"
     gene            complement(714..899)
                     /locus_tag="PLB25_21280"
     CDS             complement(714..899)
                     /locus_tag="PLB25_21280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44137.1"
                     /translation="MTKRPQSLSEEIGNSISHAAAIAAAIASVPFLMASTYRKFNRTR
                     ASMARVFGSQAIVTRSV"
     gene            complement(896..1084)
                     /locus_tag="PLB25_21285"
     CDS             complement(896..1084)
                     /locus_tag="PLB25_21285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44138.1"
                     /translation="MHLFVAIIDVNHRAVTSDGSEGLRPVEFADALPAEQFIWLRNAG
                     NIITNQLSCTTTYSKIDP"
     gene            complement(1089..>1930)
                     /locus_tag="PLB25_21290"
     CDS             complement(1089..>1930)
                     /locus_tag="PLB25_21290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44139.1"
                     /translation="NYENSAGSFQGLNLLDLRAQQAVTLGNDWDYVSKLWKKYFRLPG
                     RVIQRADEFPPLDHALGLHYRGTDKNKSLGETNFVSEDDFLALAKDFVATHPEIKTIF
                     VATDENAFVEQVRPQHPGLDIFNSGRVTHHKDLLSENNFAKGDHALLDCLLLSRCQYL
                     LKSQSALSGFAKVLNPRLEAYRVSANKLAAWNWSIPYFPDAYLPMLSSQNPACQKILA
                     RLCAGDWTEHKGMQRKFGKLFKYKNRKGYERRGYRAPLWGFDGVRMRFDSRAARLLNS
                     LGL"
BASE COUNT          425 a          543 c          539 g          423 t
ORIGIN      
        1 ttgccaatac gccacaggcc atcaccgcgt gcaggtgaat gtggcgctgc aaatcttgcc
       61 cccaggtgtg gagcaccaga ctgaacgctg gtgtgccagc gctgaccccc atccatttgg
      121 gattggcggc aaactctgcg agcgtctggg cggtgcaggc aaacagcgtg tcaatcaccc
      181 aacgcgggtg cgcgccatac aaaccgttca agctgtgtgg caaggtgaac accagatgcg
      241 catacggcac aggcagcgcc tcggccaggc ggccttgcag ccaggcatcc ttggcacgtg
      301 tgccgcaagt cgggcagtgc cgattgcggc atgaatggta ttgccagtgg ctgtggccac
      361 aggtgtcaca cgccaattgc tcaccaccga gcgcagcagt gcgacaggca actattgccc
      421 gccaggcttt ggcctgtgcg gtgctcaggg tgtgttgacg cagatacgct gggccaaact
      481 ggcgcagggc atcgaccaag gtgaccatga ccagacggct ggcgttactt gacccttcct
      541 tcgccggact gtgatctggc agcacaaccg gcaccggacc ttgatctcga ccagagcgcc
      601 aattggtgac cgtgaaaacg cagattgagc tgcgctgcgc ggtagcctgc gttcgtggca
      661 gaaccaaagc cgacttttcg ccaaaagtct caccattgag ctactgtgac ggctcaaact
      721 gaacgagtca caattgcttg ggaaccaaaa acccgtgcca tacttgccct cgtgcggttg
      781 aatttcctat aagttgaagc catcaagaac ggcacgctgg caatagctgc agcaatcgca
      841 gcagcgtggc ttatgctgtt gccgatttcc tctgataacg attgggggcg ttttgtcatg
      901 ggtctatttt gctatacgtc gtggtgcagc tcaactggtt ggtgatgatg ttgccagcgt
      961 tacgcaacca gatgaactgt tcggccggca gcgcgtccgc aaactccacc ggccgcaacc
     1021 cctcgctccc gtcactggtc accgcccggt gatttacgtc aatgatggct acaaagaggt
     1081 gcatggcgtt ataaaccgag tgaattcaat agtctggcgg ctcttgaatc gaaacgcatt
     1141 ctcacgccgt cgaagcccca gagtggcgcc cggtagcctc gccgctcgta gcccttgcga
     1201 tttttatatt tgaacaattt gccaaatttc cgctgcatac ctttgtgttc ggtccagtcg
     1261 ccggcgcaca ggcgggcgag aattttttgg cacgcgggat tctggctgga aagcatgggt
     1321 aaatacgcat cgggaaaata tggaatgctc cagttccagg ccgccagctt gttggccgag
     1381 acccgatagg cttccagccg cggattgaga accttggcaa aaccggacaa cgcggactgg
     1441 gatttgagca gatattggca gcgcgacagc agcaggcagt ccagcagcgc gtgatcgccc
     1501 ttcgcaaaat tgttttccga cagcagatct ttgtggtggg tcacccggcc ggaattaaaa
     1561 atgtccagtc cgggatgttg tggtcggacc tgctccacaa aggcattttc atcggtggcc
     1621 acaaagatag ttttaatttc cggatgcgtt gccacaaaat ccttggccag tgccagaaaa
     1681 tcatcctccg agacaaaatt ggtttcgccc agcgatttgt ttttatccgt tccccgatag
     1741 tgaagtccca gggcgtgatc cagcggcgga aattcgtccg cccgttgaat gacgcggccc
     1801 ggtagacgaa aatatttctt ccataatttg ctgacataat cccagtcatt gcccagcgtc
     1861 accgcctgct gtgcgcgcaa gtccaacagg ttgagcccct gaaaactgcc ggcactgttt
     1921 tcataattaa
//
LOCUS       DAOSLL010000715         1924 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_51546_length_1924_cov_1.764580, whole genome shotgun sequence.
ACCESSION   DAOSLL010000715 DAOSLL010000000
VERSION     DAOSLL010000715.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51546_length_1924_cov_1.764580"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..264)
                     /locus_tag="PLB25_21295"
     CDS             complement(<1..264)
                     /locus_tag="PLB25_21295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44140.1"
                     /translation="MSGAQLCPNQPSFNGLAQTHLIRNQNAVAGRFQEVQNRLELVGQ
                     EFGISRVQAVNQVCQLAAQTVVRNGATQVHCPAIEATQHQIVGI"
     gene            190..>1924
                     /locus_tag="PLB25_21300"
     CDS             190..>1924
                     /locus_tag="PLB25_21300"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HOE44141.1"
                     /translation="MIADEVGLGKTIEAGLIWTELRARQDAKRLLVVCPAMLKPKWVA
                     ELSNRFGVQAQDVDAATLLKRLEDARENPSHGFALVASLQGLRAPRGWDSDAEPPKSA
                     AAKLSQVLFEMGSEPPMIDLVVVDEAHYLRNEATQTNKFAQLLRAVTQNLVLLSATPI
                     QTSSKDLFNLLHLLDEDAFPFQASYEWNITANAPLVALRDEILRGTTVTPEKFVAVMD
                     QAKAWFRQSEQIRHLRENPPTQEQLVAPRSRAELADVLDRINPLAKVVSRTLKRDVQE
                     LRVERVVKLLSVPLTTVERVYYDRVTEAVAEMCDMEDVSTGFLLTYPQRQMASSMVAA
                     CRGWKHKLRTSIEAAQIDDFDEGVEELGLEDAIEELNNQLPGKFGLSGLMKTLIRISH
                     EAGDEDHLARSDSKYGVLIKHLQEYWQLHPYKKVVLFSFYRNTLYYLRERLSAAGVEA
                     VVLHGGMDKQATIRAFEAPSGPRILLSSEVAAEGVDLQFSSLLVNYDLPWNPAKIEQR
                     IGRIDRIGQKEDRILILNLVLADTIDSRVYELLFQRLKIFQRALGSMEVLLGDVIKQL
                     TFELLSHKLSPK"
BASE COUNT          441 a          463 c          573 g          447 t
ORIGIN      
        1 gatgccaacg atttgatgct gcgtggcctc tatggccggg caatggacct gcgtggcgcc
       61 attacgcacc accgtctgag cggcaagctg gcaaacctga tttacagcct gaacacgact
      121 aataccgaat tcttgcccta ccagttcaag ccggttttgc acttcttgga atctcccagc
      181 aacggcattt tgattgcgga tgaggtgggt ttgggcaaga ccattgaagc tgggctgatt
      241 tggacagagt tgcgcgccag acaagatgcc aagcgtttgc tagtggtgtg cccggccatg
      301 ctgaagccca agtgggttgc cgagttgtca aatcgttttg gcgttcaggc gcaggatgtg
      361 gacgcggcaa cgttgctaaa gcgccttgaa gatgcaagag agaatccgtc gcatggtttt
      421 gcgctggtgg ccagcctgca ggggctgcgt gctcccagag ggtgggattc tgatgcagag
      481 ccaccgaagt ctgcggcggc caagctgtcc caggttttgt ttgaaatggg gtctgaacca
      541 ccaatgatcg acttggtggt tgtcgatgaa gcccattact tgcgcaacga ggccacgcag
      601 accaataagt ttgcccagtt gctgcgggct gtgacgcaaa atctggtgct cttgtcggca
      661 acaccgatcc agaccagcag caaggacttg ttcaatcttt tgcacctgtt ggacgaggat
      721 gcgtttccat ttcaggcgtc ctatgagtgg aacattacgg caaatgcgcc cctggtggcc
      781 ttgcgtgatg aaattttgcg cggcacgacg gtgacccctg aaaagtttgt ggcggtcatg
      841 gaccaagcaa aagcctggtt tcgtcaaagc gagcaaattc gccatttacg tgaaaacccg
      901 cctactcagg agcagttggt cgcgccgcgc agccgtgctg aactggcgga tgtgcttgac
      961 cgtataaacc cgttggctaa ggtggtaagc agaacgctaa aacgtgatgt gcaggagctg
     1021 cgggtcgagc gggtggtcaa gctgctttcg gtcccgctaa ccactgtcga gcgggtttac
     1081 tacgacaggg tgactgaggc agttgcagaa atgtgcgata tggaagatgt ctccactggc
     1141 ttcttgttga cttacccaca gcgccagatg gcgagttcca tggtggccgc gtgccgaggt
     1201 tggaagcaca agttacgaac gagcattgaa gcggcgcaga tcgacgactt tgatgagggg
     1261 gtagaggaac ttgggcttga ggacgccata gaagagttga acaaccaatt gcccggcaag
     1321 tttgggctca gcggtttgat gaagacgctg atacgcatct cccacgaagc aggtgatgag
     1381 gatcatttgg ctcgatccga tagcaaatac ggagtactca tcaaacacct gcaggaatac
     1441 tggcagcttc acccgtacaa gaaagttgtt ctcttctctt tctaccgaaa taccctttac
     1501 tacctgcgag agaggctgtc tgccgcaggg gtagaggctg tggtgctgca tggtggcatg
     1561 gacaagcaag cgaccatacg ggcctttgaa gcgccatcgg gccccaggat tctgctgtca
     1621 tcagaagtgg cggcagaagg cgttgatttg cagttttcca gcttgctggt gaactacgat
     1681 ttgccttgga atcccgcaaa aattgaacag cgtattgggc gaattgaccg tattggacag
     1741 aaagaagatc gaatcctcat tttgaacctg gtactggccg acacgattga cagcagggta
     1801 tacgaattgt tgtttcagcg gctgaaaatc tttcagcgcg cattgggcag catggaggtt
     1861 ttgctgggtg atgttatcaa gcagctaacc tttgaactgc tgtcgcacaa gctttcaccc
     1921 aagg
//
LOCUS       DAOSLL010000716         1899 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_52511_length_1899_cov_4.175163, whole genome shotgun sequence.
ACCESSION   DAOSLL010000716 DAOSLL010000000
VERSION     DAOSLL010000716.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52511_length_1899_cov_4.175163"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(104..1006)
                     /locus_tag="PLB25_21305"
     CDS             complement(104..1006)
                     /locus_tag="PLB25_21305"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF021113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XamI family restriction endonuclease"
                     /protein_id="HOE44142.1"
                     /translation="MSVDQFNQWFLRFAPDAFRTTRVKTTEHVKAALLATTDLQSIDP
                     ATLKKNPAALSTLRMCTAPPLAVDRLIGLADANKNLVGRMESGKLPTRMAPDDLNQEL
                     AKLCRIISKLLDRDIFPWLDAATVPTCHERDRAATIVADRLCSAVANPIIRNAQEQRQ
                     LQSVGDWLNVRGYRKQNPPRDSLLSDMELGTYAFRLNLSVGKSLKVNMPIDIVIQPRT
                     PRKDGLPILIEAKSAGDFTNTNKRRKEEATKIHQLQVAYGETVPFVLFLCGYFGSDYL
                     GYEAAEGIDWIWEHRINDLLKLGL"
     gene            complement(1107..>1899)
                     /locus_tag="PLB25_21310"
     CDS             complement(1107..>1899)
                     /locus_tag="PLB25_21310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007856682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HOE44143.1"
                     /translation="EGEAANRAIAIAEVLNVPIYIVHVSCSEAADAIARARARGQRVY
                     GEVLAGHLTIDDSVYRHPDFAQAAAHVMSPPFRPKAHQEALWRGLQSGNLHTTATDHC
                     TFCASQKAAGRDDFAKIPNGCGGVEERMAVVWDEGVNTGRLTPSEFVAITSANAAKLF
                     NIYPQKGSVSVGADADLVVWDPAGSKTLSAKTQFSKNDFNVFEGRTVRGIPSHTLSQG
                     KLVFVQGDLRAERGVGRYLKRPAFNANFAASKLRAEVLAPTAVKR"
BASE COUNT          379 a          540 c          549 g          431 t
ORIGIN      
        1 cccaggcgat tgcgctcttt ggctggcttg cgtgcgtcaa tcgcaacctg aatggtttgt
       61 cgttgcgttt cgagcgaact gacatcaaaa gcaatcatgc gatttagagc cccaacttca
      121 aaagatcgtt gatgcgatgt tcccaaatcc agtcaattcc ttcagccgct tcgtagccca
      181 ggtagtcgct gccaaagtag ccacagagaa acagcacaaa aggtacagtt tctccgtaag
      241 cgacttgaag ctggtgaatt ttggtggcct cttctttgcg ccgcttgttg gtgttggtga
      301 agtcgccggc ggacttggcc tcgatcagaa tcggcaagcc gtctttccgc ggtgtcctgg
      361 gctgaatcac gatgtctatc ggcatgttca ctttgagcga tttgccaacc gacaggttca
      421 ggcgaaatgc gtaagttcca agttccatgt cactcagtaa actgtcgcgg ggaggatttt
      481 gcttgcggta accccggaca ttgagccaat cacccacaga ttggagctgc cgctgctcct
      541 gggcgtttcg gatgatcggg ttggcgaccg cactgcagag tcgatccgca acaatcgtgg
      601 cagcgcgatc acgttcgtgg caagtaggca ctgttgcggc atcgagccag ggaaagatgt
      661 cacgatccag cagtttcgag atgatgcggc agagtttggc caactcttgg ttcaaatcat
      721 caggggccat gcgggttggg agtttcccgc tttccatgcg accgaccaga tttttattcg
      781 catcggccag accaatcaga cggtcaaccg ccaacggtgg tgcagtacac atgcgcagcg
      841 tggacaaggc cgcaggattc tttttgagcg ttgccgggtc aatgctctgc aagtccgtgg
      901 tggcaagcaa agcggctttg acatgttcgg ttgttttgac acgcgtcgtg cgaaaagcat
      961 cgggcgcaaa acgcagaaac cactgattga actgatcaac cgacatcgcg atgtcggctt
     1021 tccaaagatg cggtttgtcc gcattgatcc gcctaatcat cctgatacga ttttttgcat
     1081 gaacgcctgt tcgctgcctg ttcacatcag cgctttaccg ccgtaggtgc caacacctcc
     1141 gcccgcaatt tgcttgccgc aaagttggcg ttaaaggcag gccgcttcag gtagcgcccg
     1201 accccgcgct cagcgcgcag gtcgccttgc acaaacacca gtttgccctg gctgagcgta
     1261 tggctgggga tgccgcgcac ggtgcggcct tcaaacacgt tgaagtcgtt tttgctgaac
     1321 tgggttttgg cagacaaggt tttgctgcct gccgggtccc acaccaccag gtctgcatca
     1381 gcaccgaccg atacgctgcc tttttgcggg tagatattga acagcttggc cgcattggct
     1441 gaggtgatgg caacgaattc cgacggtgtc aggcgaccgg tgttgacccc ttcgtcccag
     1501 accactgcca tgcgttcctc gacaccgccg cagccattgg gaatcttggc aaagtcgtcc
     1561 cgcccggccg ccttctggct ggcgcagaag gtgcaatggt cggtggcggt ggtgtgcagg
     1621 ttgccgcttt gcagaccacg ccacagggct tcctgatgcg ccttggggcg aaatggcggg
     1681 ctcatcacat gcgcggccgc ctgggcaaag tcggggtgac ggtagacact gtcgtcaatc
     1741 gtcaggtgcc cggccagcac ctcgccataa acccgctgac ccctggcgcg ggcgcgggca
     1801 atcgcatcag ccgcctcgct acacgacaca tgcacgatgt agattggcac gttgagcacc
     1861 tcggcaatgg caatcgcgcg gttggcggct tcaccttcg
//
LOCUS       DAOSLL010000717         1862 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_53973_length_1862_cov_3.632540, whole genome shotgun sequence.
ACCESSION   DAOSLL010000717 DAOSLL010000000
VERSION     DAOSLL010000717.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53973_length_1862_cov_3.632540"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(332..640)
                     /locus_tag="PLB25_21315"
     CDS             complement(332..640)
                     /locus_tag="PLB25_21315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44144.1"
                     /translation="MSLIAAFPAMSVTLALAKRNLTVQLRQVEEGGNSGYAVGTKAGE
                     ALMTEQIVNVGNGEMAVFNKMCRFDVPRQSASVIHLAWDLNSPSFFLPERGPQGFHPA
                     "
     gene            complement(890..1477)
                     /locus_tag="PLB25_21320"
     CDS             complement(890..1477)
                     /locus_tag="PLB25_21320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44145.1"
                     /translation="MEQALEGLKPLVTIDVATTTLTYTVSSQVQPVDDLSVVNADEQA
                     KKKTKPKLTAKPKAEKTKAVKAKAGEAKVVAPVLAVEAAKLPATGAELWLKCIGRKKV
                     TVAQIVDAALKRLKLEESARDVIATRAKAWTYSAAKKGDLIEAGMRDGSKLYQLSAVK
                     DESPVVNGQAADPVAAVEAAKAVEVAPVAEAGKAD"
     gene            complement(1646..>1862)
                     /locus_tag="PLB25_21325"
     CDS             complement(1646..>1862)
                     /locus_tag="PLB25_21325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44146.1"
                     /translation="SDLCQTTRNFAEWSVACELVSNVFDIYKGCVQGRSKVAVVGRNS
                     MQTHWSRSVHHSTHCADKKVHSDHIIC"
BASE COUNT          425 a          526 c          456 g          455 t
ORIGIN      
        1 ttgcgaccag gcttgagcgg cctcgtttaa cgcgacaaaa atctgccgcg catcgaacca
       61 ggcgggagca aacgcatgct gatacacaaa aacaagtggc gatgccatcg ctccaggttg
      121 ccatctctcc cggcagacat tgcgctatct cctggtcacg caccggctca actgccaatg
      181 atggctgtgc cgctgcacaa cagatcagga aaacaaaggt gaagaaaagt cgattgcttg
      241 acatccggtt ggcctgcact taagtatgcc aaaagaagca tttcacagaa ggctcgtgca
      301 tgcgttcatc acacataaaa aaaccggcac ttcaggccgg gtggaaccct tgcgggcctc
      361 gttcagggag gaaaaacgag ggggagttta agtcccaagc caaatgtatc actgaagcgg
      421 attgcctcgg tacatcaaat cggcacatct tgttgaatac cgccatttca ccatttccca
      481 cattgacgat ctgctcagtc atcaacgctt caccggcttt ggtgccaacc gcgtagcccg
      541 agttgccgcc ctcctctacc tgccgcaatt ggaccgtcag gttgcgtttt gccagcgcta
      601 gcgtgactga catggcaggg aacgcggcga tcaaagacac acatacagcg gtgatgtgaa
      661 caaaccatcg agggaacatg catttcacga tgctgtgtca atcggtatga acgccctgcc
      721 cgcccggtat tcaaggttta gccagggggc atcaagcctg ccttttccaa aatatcgagc
      781 gtcgcttcat ctggtctttc gtggaagaat tgctccaagc cttgttgcaa cactgtctgg
      841 tgaagcctta cttatttgaa tccagccagc gcaagaccat tgcatccgtt cagtccgcct
      901 tgccagcttc cgccaccggg gcaacttcaa ccgccttggc agcttcaaca gccgcaactg
      961 gatcggcggc ttgaccattg acaactgggc tctcgtcctt gacggcggac aactggtaca
     1021 gcttggagcc atcacgcatc ccagcttcaa tgaggtcacc tttcttggcg gccgaatagg
     1081 tccaggcttt ggcacgggta gcgatcacat ctctcgctga ttcttccaat ttaagtctct
     1141 tcaacgccgc atcgactatt tgtgcgacag taactttctt gcgaccaatg cacttgagcc
     1201 aaagctcggc acctgttgcc ggtaacttgg cagcttccac cgccagcacc ggcgctacca
     1261 ccttggcctc accagccttt gcctttactg ctttggtttt ttcagctttg ggcttggccg
     1321 tcaatttggg cttggtcttt ttctttgctt gttcatcggc attaacaacc gataggtcat
     1381 caaccggttg cacctgggac gatacggtgt aagtcaaagt tgttgtggct acatcaatcg
     1441 tgacgagcgg cttgagacct tcgagcgcct gctccaaatt gatcaagtgc tgacgagtgc
     1501 gctcaatctc ctgcttgatg tgattcgatg cttcaaacaa tgtttgcatg agatgtgttt
     1561 tcctttttgg ttgcgccgaa cgacaaatgg ctcggttaac ctccgatgat attcgagtgg
     1621 cagcagcatt tctattgtta actgctcaac agattatgtg atcgctgtgg acctttttgt
     1681 cggcgcaatg tgtggaatgg tgcactgacc gcgaccagtg cgtctgcatc gaattgcgtc
     1741 cgacaaccgc cactttgcta cgaccctgca cacagccctt gtaaatgtcg aaaacattgg
     1801 acaccaactc gcaagccacc gaccactcgg caaagttgcg cgtagtctgg cacaaatcgc
     1861 ta
//
LOCUS       DAOSLL010000718         1855 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_54250_length_1855_cov_8.373889, whole genome shotgun sequence.
ACCESSION   DAOSLL010000718 DAOSLL010000000
VERSION     DAOSLL010000718.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54250_length_1855_cov_8.373889"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(505..1056)
                     /locus_tag="PLB25_21330"
     CDS             complement(505..1056)
                     /locus_tag="PLB25_21330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HOE44147.1"
                     /translation="MNRVVNWSDSRPLQYLQGYPAGTLAQVEKLLQDNQVADWLLRKY
                     PDCHSVRTDRALFDYVQALKTRHLRGAEPLAKVAFDAKLQVVQHALGTHTTIARVQGS
                     KLKAKREIRVASLFKQVPDEFLKMIAVHELAHLKERAHDKAFYQLCSYMEPRYHQLEF
                     EVRVYLTHLEASGARLWGAVGIS"
     gene            complement(1100..>1855)
                     /locus_tag="PLB25_21335"
     CDS             complement(1100..>1855)
                     /locus_tag="PLB25_21335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HOE44148.1"
                     /translation="EGISVRHNPEFTMMEFYAAYWDYQDLMTFTEALVRDAAQKAVGK
                     LELSYGGRPVDLSQPFARLTIHEAICKFTEGGETLDDGTGLKVDNATWLLNALRRLGM
                     TEAKHHLSGRSLAGLQVLYFEETVEEKLWHPTFICEHPVEISPLARESDHKPGVTERF
                     ELYITGREFGNGFSELNDAQEQAARFQAQVTAKDSGDLEAMYYDHDFVRALEYGMPPT
                     GGCGIGIDRLMMLLTDSASIRDIILFPALRRES"
BASE COUNT          363 a          531 c          552 g          409 t
ORIGIN      
        1 tgtgtaggag tcgcgccctc gcgacgaagg ctcatcgatt ccggctcgtc cggctaagga
       61 tgtggtcaga aacaacttcc cctatttgca ccggcggatt tgggcgcaca gcgtcgttgc
      121 aagacgcttg tttagccagt tgcacggcgc gtcttgcgtc tagccgtgca tcccaactcc
      181 ttggtgcaaa acggggagtt attcccgaca acatccttag ctccaatgcg gttcatccaa
      241 tgggcggtgc cgccatgcac ccgattgaat cactggtaac tcgctttggg gggttgctcc
      301 gcctggcggg caatcccaag gcaaggcgag ataacaacga catggatgat ttggaaatgg
      361 aacgggaagc acaaagctga actgctttaa gcctgttgcg cggacttgtt tgaccagccc
      421 ggcaaagggt ctcgcgcaat gggtcttgtt attggctggt gggcgtcaac tgggtcatcc
      481 tgccagcctg cgcagggcca gacactaaga aatgccgact gccccccaca gccgagcccc
      541 ggacgcttcg agatgcgtca ggtacacacg cacttcaaac tcaagctggt ggtagcgcgg
      601 ttccatgtag ctgcagagct ggtagaacgc cttgtcgtgc gcacgttcct tgaggtgggc
      661 gagttcatgc acggcgatca ttttcagaaa ttcatcgggc acctgtttga acagtgaagc
      721 gacgcggatt tcgcgcttgg ccttgagctt gctgccctgc acgcgtgcga tggtggtgtg
      781 ggtacccagg gcgtgttgca ccacctgcag cttggcatcg aacgccacct tggccagcgg
      841 ttcggcgcca cgcaggtgcc tggttttgag cgcctggacg tagtcaaaca gcgcacggtc
      901 ggtgcgcacg ctgtggcaat cggggtattt tctaagaagc cagtctgcca cctggttgtc
      961 ttgcagcagt ttttcgacct gtgccaaggt gcctgcggga tacccctgca aatattgcaa
     1021 aggccgcgag tcagaccagt ttactactct attcatagct gtcagcgcaa ttgcaataag
     1081 ggctagatgc caattttggt caagattccc ggcgcagcgc ggggaacaag atgatgtcgc
     1141 gaatgctggc gctgtcggtc aacaacatca tcaggcggtc aatgccgatg ccgcaaccgc
     1201 cggtgggcgg catgccgtat tccagcgccc gcacaaagtc gtggtcgtaa tacatggcct
     1261 caaggtcacc gctgtctttg gcggtgacct gggcctggaa gcgggccgcc tgctcctggg
     1321 catcgttgag ttcgctaaag ccgttgccga actcacgccc ggtgatgtag agctcaaagc
     1381 gttctgtcac gcccggcttg tggtcactct cgcgggccag tggcgatatt tcgaccggat
     1441 gctcgcagat aaaggtcggg tgccagagct tttcttccac cgtttcctca aagtacagca
     1501 cctgcaagcc agccaggctg cgaccagaca ggtggtgctt ggcctcggtc atgccgagtc
     1561 tgcgcagggc attgagcagc catgtggcgt tgtcaacctt caggccagtg ccatcatcaa
     1621 gcgtttcgcc accttcggtg aatttgcaga tggcttcgtg aatcgtcagc cgggcaaacg
     1681 gctggctcag atcaaccggc ctgccgccat aactcagttc gagcttgcca acggccttct
     1741 gcgccgcgtc gcgtaccagc gcttcggtga aagtcatcag gtcctggtaa tcccaatacg
     1801 ccgcatagaa ctccatcatg gtgaactcgg ggttgtggcg gacgctgatg ccttc
//
LOCUS       DAOSLL010000719         1851 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_54411_length_1851_cov_4.026726, whole genome shotgun sequence.
ACCESSION   DAOSLL010000719 DAOSLL010000000
VERSION     DAOSLL010000719.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54411_length_1851_cov_4.026726"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="PLB25_21340"
     CDS             complement(<1..511)
                     /locus_tag="PLB25_21340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgF trimerization domain-containing protein"
                     /protein_id="HOE44149.1"
                     /translation="MKLRKLFSGAGRSLALAAALVWMAPAGAGLFDDEEARRAILDLR
                     QRIEASSQALEQKMAIESKRANEEDAQLRRSLIELQNQLEAVKADQAKLRGQNEQMAR
                     DLAELQRKQKDDAQAFGDRLRKLEPAKVTFDGREFTAEQVEKRDFETAMAIFRKGEFA
                     NSEVAFVDFI"
     gene            complement(508..1038)
                     /locus_tag="PLB25_21345"
     CDS             complement(508..1038)
                     /locus_tag="PLB25_21345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HOE44150.1"
                     /translation="MKKLVSIVALVAMLSACGSAVKLDNVPVEDKSGSSVGAGGASTA
                     GSGSGVGNGGVASVDLGKSAQDMSLQSATRIVYFDYDSFAIRPEFVSVIENHARFIKG
                     DRNRKVVIEGHTDERGGREYNLALGQKRAEAVRRALGVSESQVEAVSFGKEKPAVPGS
                     TEAAMEKNRRAEIVYR"
     gene            complement(1062..>1851)
                     /gene="tolB"
                     /locus_tag="PLB25_21350"
     CDS             complement(1062..>1851)
                     /gene="tolB"
                     /locus_tag="PLB25_21350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016446823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HOE44151.1"
                     /translation="RIAYITKGKGYTLWVADADGENPQSALTSPEPIISPSWSPNGQQ
                     LAYVSFESRKPVVYVHEVASGKRRLVANFKGSNSAPAWSPDSRTLAVTLSRDGGSQLY
                     LMDSAGGEPRRLAQSPSIDTEPTFTADGKTIYFVSDRGGAPQIYRMPASGGNADRVTF
                     SGTYNISPSLSPDGRWLAYVSRVNGAFKLHVMDLASGAANAITDTSADENPSFSPNSR
                     LIIYATQQQGREALMTTTLDGKIKARLAGQGGDIREPDWGSVQR"
BASE COUNT          394 a          565 c          476 g          416 t
ORIGIN      
        1 tgatgaaatc cacaaacgca acttccgagt tggcaaattc ccctttgcga aaaattgcca
       61 tggccgtttc gaagtcccgc ttttccacct gttcagccgt gaactcacga ccatcaaaag
      121 tcaccttggc aggctccagt ttgcgaaggc gatcaccaaa tgcctgcgca tcatctttct
      181 gcttgcgttg caactctgcc agatcgcggg ccatttgctc gttctgccca cgcagcttgg
      241 cttggtcggc tttcactgcc tccagctggt tttgcaactc gatcaggctt cggcgaagct
      301 gggcgtcctc ttcgttcgca cgcttggatt cgatcgccat tttctgttcg agggcctgac
      361 tggaagcctc aatcctctgg cgcaaatcca gaatagccct gcgtgcctct tcgtcgtcaa
      421 acaggccggc acccgcggga gccatccaga ccagtgcagc agccaacgct aggcttcggc
      481 cagcacccga aaacagctta cgcaatttca tcggtaaacg atttcagcgc gacggttttt
      541 ttccatggca gcttcagtgg aaccgggtac cgcaggcttt tctttgccaa aactcacggc
      601 ttcaacttgt gactcgctca cacccaatgc gcgacgaacg gcctcagcgc gcttctggcc
      661 gagtgccagg ttgtattccc gaccaccgcg ctcatcggta tggccttcaa tgaccacctt
      721 gcggttgcgg tcgcctttga tgaaacgcgc atggttctca atgacggaca caaactcagg
      781 acggatcgcg aaactgtcgt agtcgaaata gacgatgcgg gtcgccgact gcaggctcat
      841 gtcctgagcc gatttgccca gatcgaccga tgcgacgcca ccgttgccaa caccacttcc
      901 gctacccgct gtagaggcac caccggcacc taccgaggat ccagacttgt cttctaccgg
      961 aacattgtcc agcttgactg cagaaccgca tgcactgagc attgctacca gtgcgactat
     1021 tgataccaat tttttcattc aagactccta agttattcca attatcgctg caccgagccc
     1081 cagtccggct cccggatatc cccgccctgc cccgcaaggc gggccttgat ctttccatcc
     1141 agtgtcgtgg tcatcaatgc ttcacgccct tgttgctggg ttgcatagat gatgagcctg
     1201 ctgttaggcg aaaaactggg gttttcatcc gctgacgtgt ccgtaatggc attggcagca
     1261 ccactcgcga gatccatgac gtgcagttta aacgcaccgt tgacgcgaga aacataggcc
     1321 agccaacgcc catctggact caaagacgga gagatgttgt acgttcccga aaaagtaacc
     1381 ctgtccgcat tgccacctga tgcgggcatg cgatagatct gtggagcacc cccacggtca
     1441 ctcacgaagt agatggtctt tccatcagcg gtaaaggtag gctccgtatc aatcgaaggg
     1501 gactgcgcca gacggcgcgg ctcgcctccg gccgaatcca tgagatacag ctgcgagccg
     1561 ccatcccgac tcagcgtgac agcgagcgtc cggctatcgg gtgaccaggc tggcgcgctg
     1621 ttagatccct tgaagttggc tacgagccgg cgttttccgg atgcaacctc gtgaacgtaa
     1681 acaacaggct tgcgcgactc aaatgacaca tacgcaagct gctgcccgtt gggcgaccat
     1741 gacggcgaaa tgatgggctc agggctggtc aatgcacttt gaggattttc accgtctgca
     1801 tcggcaaccc acaaggtgta gcccttgcct ttggtgatgt aggcaatgcg c
//
LOCUS       DAOSLL010000720         1845 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_54629_length_1845_cov_5.030168, whole genome shotgun sequence.
ACCESSION   DAOSLL010000720 DAOSLL010000000
VERSION     DAOSLL010000720.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54629_length_1845_cov_5.030168"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..1148
                     /locus_tag="PLB25_21355"
     CDS             78..1148
                     /locus_tag="PLB25_21355"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HOE44152.1"
                     /translation="MQARQLNDWSAVPKAAARQQVGPLLYWHLKEYGIAYPQDIRRAL
                     AAMYARQKAIASAQAETLAEIIKTFADAGIESVVIKGGALAHIIYPEPGLRPMEDLDI
                     LVSPGQATAAHGLLLALGFRAPLPSSRFDLLQHHLPLAQRTCGEMTVSVELHTSAFNL
                     LMQDKLSMDNMMRPLFPYKALGQPMHTLNPVQMVWMQYLGLRKLAEPLRYIHLADLVG
                     MAERLIDDIDWAKLQRDYPDLWNAYEAVHAFSPLSSSLCQRLELSSDAPPKMSAIGQD
                     FRGWPRQSFSDQTSARDKRILLGRSVIPPEWWARLVYGVRTSRSMAGILFYHHPATFF
                     QQGVRRLYLGPVNRLGFFKGPV"
     gene            1173..1451
                     /locus_tag="PLB25_21360"
     CDS             1173..1451
                     /locus_tag="PLB25_21360"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HOE44153.1"
                     /translation="MNLFDSSFRPKDGLEAEDTGDEILIYLPGGDKTLYLNETAALVW
                     RLCDGARSGAEIVELLTEAYPEAEAVIQGQVQDALSDFIARGIVESTA"
     gene            1435..>1845
                     /locus_tag="PLB25_21365"
     CDS             1435..>1845
                     /locus_tag="PLB25_21365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014426623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44154.1"
                     /translation="MKVPPEMSGVVRIAFVGNELQFCTDSALVAEAATFVFRHHLSPP
                     VDDPSQTVDVVADGDGFRFVANGQVVAIAMNLAELPVTLMQVGQQRLIFKETTHAMLH
                     AAVLVKDGCGLLLPAVAGSGKTTLTAWLLGHGYGL"
BASE COUNT          394 a          473 c          526 g          452 t
ORIGIN      
        1 catttcgcaa aatcaagtta ggtgtaaaca ttatttacgg ataagttctt agtaagaggc
       61 gtgccgatct gttacttgtg caggccagac agctcaacga ttggtcagcc gttcctaaag
      121 cggcggcacg ccagcaagtt ggtccgctgc tttactggca tttgaaggaa tatggcattg
      181 cctatccgca agacatcagg cgtgccctgg ctgccatgta cgcccggcaa aaagcgattg
      241 ccagcgcgca agccgagact ttggccgaga tcatcaagac gttcgctgac gctggcatcg
      301 aatccgtggt gatcaagggc ggtgcgctgg cccacatcat ctatcccgaa cctggcttgc
      361 ggcccatgga agacctcgat attttggtca gtccagggca ggctaccgcc gcgcacggct
      421 tgctgctggc attgggcttt cgggcacctc tgccgagcag ccgctttgac ctgctgcaac
      481 accaccttcc gctggctcag cgaacttgcg gcgagatgac tgtcagtgtt gaactgcata
      541 ccagtgcatt caatttattg atgcaagaca aactgtcgat ggacaacatg atgcgcccct
      601 tgtttcccta caaagcgcta gggcagccga tgcacacact caatccggtt caaatggtgt
      661 ggatgcagta tcttgggttg cgcaaactgg ccgaacctct tcgctacatt catctggccg
      721 atctggttgg catggctgag cggctgattg acgacattga ttgggcgaaa ctgcagcgtg
      781 actatcccga tctgtggaac gcctacgaag cggttcatgc gttttcaccg ttgagcagta
      841 gcctgtgtca gcgactggaa ctgtcatcag acgcgccgcc aaagatgtcg gccatcggac
      901 aggacttcag gggctggcca cgccagagtt tcagtgatca gaccagtgca cgtgacaagc
      961 ggattttgct tggcagatca gtcattccgc cagaatggtg ggcaagactg gtttatggtg
     1021 tgcgcaccag ccgcagcatg gctggtattt tgttttacca tcatccggcg acgttttttc
     1081 aacaaggtgt gcgtcgcctt taccttggac ctgtcaaccg gctcggtttt ttcaaagggc
     1141 cggtttgatt acaacaactg tgagaacgca gcatgaattt atttgacagc agttttcgcc
     1201 caaaagacgg gttggaggca gaagacaccg gagacgagat tctcatctat ctgccaggcg
     1261 gcgacaaaac gctttatttg aatgaaactg cagccttggt ttggcggctt tgtgatggtg
     1321 cgcgttcggg tgccgaaatt gttgaattgt tgaccgaagc ctatccggaa gccgaagccg
     1381 tcattcaggg gcaagtgcaa gatgcgttgt cagactttat cgccaggggc attgttgaaa
     1441 gtaccgcctg aaatgtctgg cgtggtgcgg atcgcatttg ttggcaacga acttcaattt
     1501 tgcactgaca gcgcattggt tgcagaagct gccacctttg tttttcggca tcacttgtcc
     1561 ccgccggtcg atgatcccag tcagacggtt gatgttgtag cggacggcga cgggtttagg
     1621 tttgtggcga atggtcaagt cgtggccatc gccatgaatc tggctgagct accggtcacg
     1681 ctgatgcagg tcggacagca gcgacttatc ttcaaggaaa ccactcacgc catgctgcat
     1741 gctgccgtgc tggtcaagga cggttgcgga ttgcttttac cagccgttgc cggatcgggc
     1801 aaaaccacct tgacggcctg gctgctcggt catggttatg gtctg
//
LOCUS       DAOSLL010000721         1834 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_55084_length_1834_cov_2.494660, whole genome shotgun sequence.
ACCESSION   DAOSLL010000721 DAOSLL010000000
VERSION     DAOSLL010000721.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55084_length_1834_cov_2.494660"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1803)
                     /locus_tag="PLB25_21370"
     CDS             complement(<1..1803)
                     /locus_tag="PLB25_21370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HOE44155.1"
                     /translation="MDAPGSYRRFDGSIMDIRKHVRKSGQQCEFYAVRARPMRGTQRY
                     EPEITDIGLHLAATLEATEDCLVLTDNEGSILNMNRRFSQLWPLPQELLVERDDRGIV
                     VFIDGLLRDEPGQSHQFTMLQQVIELQTEPHGNTFETLTLKDNRVIECFSHPARNHDR
                     TIGRVFCFRDVTERIQYETSLKEARDKAEMAMRGKAQFLANMSHEIRTPMNGILGMAQ
                     MLLQPNLQEEQRNDYARTVLSSGQTLLALLNDILDLSKIEAGKFQIESTAFAPEALMH
                     ETINLFAGAAQTKGLELDCQWHGASDQRYLADSHRLRQMLSNLVGNAIKFTRSGQVRI
                     EARALERTEGTSVLEFAVSDTGIGIPAHKLDLLFKPFSQTDSSTTREFGGSGLGLSIV
                     SHLVKAMGGETGVSSELGQGSRFWFRVKVQNIAENRNSRKSERLPLDTNSDNVTTVLQ
                     GHVLVVEDNLVNCMVIESLLNSVGLIVSVVHDGQKAVELIEHHDGGANADTPTRPDLI
                     LMDLHMPVMDGYTATERIRQWEVSIQHSPLPIIALTADAYEEDRQHCLAIGMNDFLCK
                     PIALDELKIALARWLPAGTAAQAVNPGPKTLKPLD"
BASE COUNT          380 a          503 c          505 g          446 t
ORIGIN      
        1 gtccaaaggc ttgagggtct tggggcccgg gttcaccgct tgcgccgcag tgcctgccgg
       61 cagccacctg gccagtgcta ttttgagttc atccagggca atcggtttgc acagaaagtc
      121 gttcattcca attgccagac aatgctgtcg gtcttcttca taagcatcag cagtcagtgc
      181 aatgattggc agaggcgaat gttggatact tacttcccat tgccggattc tttccgtggc
      241 ggtgtagccg tccatcaccg gcatgtgcag gtccatcagg atcagatcgg ggcgtgtggg
      301 cgtatcggcg ttggcgccgc catcatgatg ctcaatgagc tcaactgcct tttgtccatc
      361 atgaaccaca gaaacgatca gccccacact gttcaaaagt gattcgatca ccatgcagtt
      421 gaccaggttg tcttccacaa ccagcacatg accttgcagt acagtggtta cgttgtccga
      481 gttggtatca agtggcagtc gctcggattt gcgactgttc cgattctcgg caatgttttg
      541 aactttgact cgaaaccaga agcgggagcc ttgaccgagt tcgctgctga cgccagtctc
      601 gccccccatg gcctttacca ggtgactcac aatcgacagt cccagaccag agccaccaaa
      661 ctcacgggtg gttgaactgt cagtttgtga aaatggctta aacaataaat ccagtttatg
      721 ggctgggatg ccaatgccgg tatcgctgac ggcgaactcc aacacgcttg tgccctcggt
      781 acgttctaac gctctggctt caatacggac ttgacccgag cgggtgaatt tgatggcgtt
      841 gccaacaagg ttcgagagca tctggcgcag gcggtgtgaa tcggccagat agcgttgatc
      901 agatgccccg tgccactggc aatcgagttc caaacctttg gtctgggccg ccccggcaaa
      961 caggttgatg gtttcatgca tcaatgcctc aggggcaaat gcggtgctct cgatctggaa
     1021 ttttccagct tcgatctttg acaagtcaag gatgtcgttg agcagggcca gcagagtctg
     1081 cccgctcgac aggacggtgc gggcgtagtc attgcgctgc tcctcttgca gatttggctg
     1141 cagcagcatt tgcgccatac ccagaatgcc gttcatgggc gtccgaattt catggctcat
     1201 gttggccaga aattgcgcct tgccgcgcat cgccatttct gccttgtcgc gtgcttcctt
     1261 cagcgaggtt tcgtactgga tacgctcggt gacatcgcga aaacaaaaaa cccgaccaat
     1321 ggttcggtcg tggttgcgcg caggatgaga aaaacattca attactcggt tgtccttcag
     1381 ggtcagcgtc tcaaaagtgt tgccatgcgg ctctgtctga agttcgatca cttgttgaag
     1441 catggtgaac tgatgcgact ggcccggctc atcacgcaac aaaccgtcaa taaataccac
     1501 gatgcctcgg tcatcgcgct ccaccaacag ttcttgaggc agcggccaaa gctgggagaa
     1561 gcggcgattc atattcagaa tggagccttc gttgtcggtc agcaccaggc agtcttctgt
     1621 ggcctccagg gtggctgcca ggtgcagccc gatgtcagtg atttcgggtt cgtaacgttg
     1681 tgtgccgcgc atgggccgcg cccgcacggc atagaactcg cattgctgac cggacttgcg
     1741 cacatgtttg cgtatgtcca tgattgaccc atcgaacctg cggtagctgc ccggcgcgtc
     1801 cattggtgtc tcgctggtac gcatttcatc ccag
//
LOCUS       DAOSLL010000722         1811 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_56034_length_1811_cov_3.907745, whole genome shotgun sequence.
ACCESSION   DAOSLL010000722 DAOSLL010000000
VERSION     DAOSLL010000722.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56034_length_1811_cov_3.907745"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="PLB25_21375"
     CDS             <1..478
                     /locus_tag="PLB25_21375"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HOE44156.1"
                     /translation="EGGGWTSDTVRFLVERGIPVCAHPGLTPQTVHALGGYRVQGKTV
                     EAAAIMRQHALELQDAGAALMVLEMVPATLAAELTTELPHCPTIGIGAGNGTAGQVLV
                     MHDMLGLNLGKMPKFVRNFMAELPGIPEHHGIKDAMLAYVKAVKSSAFPDNSKHAW"
     gene            580..891
                     /locus_tag="PLB25_21380"
     CDS             580..891
                     /locus_tag="PLB25_21380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44157.1"
                     /translation="MSIPTPHAGRAFSKQQDKAEVLPVRPKGKPGQGEFSSKIPSSLY
                     SSLFGLCVEYPAITLVKKGGVNAFFLVRVTGVKNIHLFLRKTQSGLTARQGKFVLSLN
                     R"
     gene            1011..1286
                     /locus_tag="PLB25_21385"
     CDS             1011..1286
                     /locus_tag="PLB25_21385"
                     /inference="COORDINATES: protein motif:HMM:NF015578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family NRPS accessory protein"
                     /protein_id="HOE44158.1"
                     /translation="MQADEGNHGEKFDAVINEAPQSSMWLADKEVPAWWQKNGVTGSR
                     AHCLEQISVIWTEMRPMPVRRQRDDFVFTAKNGCGTHSPNSTDVLPS"
     gene            1471..>1811
                     /locus_tag="PLB25_21390"
     CDS             1471..>1811
                     /locus_tag="PLB25_21390"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HOE44159.1"
                     /translation="MSQWNAKVKMPSEGQQGRGAQQDRGGAASAGKSRAGNSDLKAAL
                     DSARALFQAKRHDEALRAFEAILEKNPDAVPAYLGIGNVHAAQGHLDDALEYYAGALH
                     IKKDFAPAMVMS"
BASE COUNT          418 a          460 c          530 g          403 t
ORIGIN      
        1 ggagggcggc ggctggactt cagacaccgt gcgttttctg gtggagcgcg gcattccagt
       61 gtgcgcccat ccgggcctga ccccgcaaac cgtgcacgcc ttgggtggct accgggtgca
      121 aggtaaaacg gtcgaggccg ccgccatcat gaggcagcat gcgcttgaac tgcaggacgc
      181 gggggccgct ttaatggtgc ttgaaatggt gccagcaacc ctcgctgcag aactcaccac
      241 cgagttgcca cactgcccga ccattggcat cggtgctggt aacggcacag caggccaggt
      301 gctggtgatg cacgacatgc tgggtctgaa tctcggcaag atgcccaagt ttgtgcgcaa
      361 tttcatggcc gagctacccg gcatccccga gcaccatggt atcaaggatg ccatgctggc
      421 gtatgtcaag gcggtcaaat ccagcgcatt tcctgataac agcaagcacg cctggtgatc
      481 actttggcag gcgctgcttg ccatcggtga accagcatgt tcttgcaatg actgattgtg
      541 tactctgttg tgtggagttt gcgttgcttc gagtcactta tgagcatccc aactccgcac
      601 gcgggccggg cgttctcaaa gcaacaggac aaggccgaag tgcttcctgt caggccaaaa
      661 ggcaaaccgg gacaaggtga gttttcatcc aaaatcccga gttcacttta ttcgtcgctg
      721 tttgggttgt gcgtagaata cccggctata acactggtca aaaagggcgg agtgaacgcg
      781 ttttttcttg tgcgggtgac tggcgtgaag aatattcact tgtttttgcg aaaaacgcaa
      841 tctgggctca ccgcgaggca gggcaaattt gttctctcgc taaatcgttg atttaccgtc
      901 aatcccgcgc aggcgggaat gacgaagatg ccataagaac aaagttaccc tgcaacacga
      961 ggcaacggct cgtgttgttt ttttcaacac agtgtttctg gggcccacgg atgcaagcgg
     1021 acgaaggcaa tcatggcgag aaattcgacg ctgtgatcaa tgaagcacca cagagctcga
     1081 tgtggttggc tgacaaagag gtgccagcct ggtggcaaaa aaatggggtg actggttcgc
     1141 gcgctcactg cctggaacaa atatcggtga tttggacgga aatgcggcct atgcctgttc
     1201 gtcggcaacg ggacgatttt gtattcactg ccaaaaatgg ctgcgggacg cattcaccga
     1261 attcaactga tgtcctgcca tcctaagctc aggtgcgcta ttcgcaccgt catctcaatg
     1321 gattaaaaac tgctacacaa ttgaaggttt tggcagtggt caaactgcct gcgccggttg
     1381 ttcaaaacgt agcgcaccgt atgctgcgag gttgtcggtg cactgctcat ttaatgcctg
     1441 ataacggttt catgaaaaag gaaaacacac atgtcgcaat ggaatgccaa ggtcaagatg
     1501 ccatcggaag gtcagcaagg gcggggtgcg cagcaagaca gaggcggtgc cgcttcggcc
     1561 ggcaagagta gggctgggaa ttcagatctc aaagctgcgt tggactcggc gcgcgccttg
     1621 tttcaggcaa agcgccatga tgaggcgtta agggcattcg aggccattct ggaaaaaaat
     1681 cccgatgccg tgcctgccta tctgggcatc ggcaacgttc acgcggcgca gggtcatctc
     1741 gatgatgcac ttgagtacta tgctggcgcg cttcacatca agaaggattt tgcgccggca
     1801 atggtcatgt c
//
LOCUS       DAOSLL010000723         1806 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_56234_length_1806_cov_4.206739, whole genome shotgun sequence.
ACCESSION   DAOSLL010000723 DAOSLL010000000
VERSION     DAOSLL010000723.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56234_length_1806_cov_4.206739"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1233)
                     /gene="dxs"
                     /locus_tag="PLB25_21395"
     CDS             complement(<1..1233)
                     /gene="dxs"
                     /locus_tag="PLB25_21395"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011795140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HOE44160.1"
                     /translation="MMNNPYPLLQTIHAPDDLRRLERAQLAPLATELRAYLLDSVAKT
                     GGHLSSNLGTVELTVALHFVFNTPHDRLVWDVGHQTYPHKILTGRRERMASLRQLGGL
                     SGFPQRGESEFDAFGTAHSSTSISAALGMAVASRLKGEDRHAVAVIGDGAMTAGMAFE
                     ALNNAGVSDCNLLVILNDNDMSISPPVGALNRHLARLLSGQFYAAAKNAGKTVLKGAP
                     PLFQLAKRFEESAKGMVKPITLFEKFGFNYVGPIDGHDVETLVEVLENIKHLKGPQFL
                     HVVTKKGQGYKLAEADPVAYHGPGKFDPAVGLQKSSAFAKPSFTEVFGQWLCDQAAAD
                     MRLVAITPAMREGSGMVEFETLFPGRFFDVGIAEQHAVTFAAGLACEGLKPVVAIYST
                     FLQRAYDQLIHDVAIQNLP"
     gene            complement(1314..>1806)
                     /locus_tag="PLB25_21400"
     CDS             complement(1314..>1806)
                     /locus_tag="PLB25_21400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011804474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HOE44161.1"
                     /translation="PVQRQAHLCRLLSRAAGCAGMAGGQAIDLASVGQQLTEAQLREM
                     HRLKTGALLQASVMMGLACGQPTAMATAALQEFGAAVGLAFQVVDDILDVTADSATLG
                     KTVGKDADNNKPTYVSLMGLAPAGSFAQDLLAQALAALVRSGLTDTRALQALAVMVVQ
                     RSN"
BASE COUNT          363 a          580 c          515 g          348 t
ORIGIN      
        1 cggcaggttt tgaatcgcca catcgtggat caactggtcg taggcacgct gcagaaaagt
       61 cgagtagatc gccaccaccg gtttcagccc ctcacaggcc agcccggcgg caaaggtcac
      121 cgcgtgttgc tcagcaatgc cgacatcaaa gaagcgcccc ggaaacaatg tctcaaactc
      181 caccatgccc gagccctcgc gcatcgccgg ggtgatggcc accaggcgca tgtcggctgc
      241 cgcctggtca cacagccatt ggccaaacac ctcggtgaag ctgggtttgg caaacgcgct
      301 tgacttctgc aggcccaccg ccggatcaaa cttgcctggg ccgtggtagg ccaccgggtc
      361 ggcttcagcc agcttgtaac cctgaccttt tttagtgacc acgtgcaaaa actgcggccc
      421 cttgaggtgt ttgatgttct ccagcacctc gaccagcgtt tccacgtcgt gcccgtcaat
      481 cgggccaaca tagttgaaac cgaatttttc gaacagcgtg atcggcttga ccatgccctt
      541 ggccgactct tcaaaacgct tggccagttg gaacaacggc ggcgcaccct tgagcacggt
      601 tttgccagcg tttttggcgg ctgcataaaa ctggccgctc agcagccggg ccaggtggcg
      661 gttgagcgca cccacgggcg ggctgatcga catgtcgttg tcgttcaaaa tcaccagcag
      721 attgcagtcc gacacaccag cgttgttcag cgcctcaaat gccatgcctg ctgtcatggc
      781 accgtcacca atgaccgcca ccgcatggcg gtcttcgccc ttcagcctgg atgccaccgc
      841 catgcccagc gcagcggata tcgaggtgct ggaatgcgcg gtgccaaagg catcaaattc
      901 gctctcgcca cgctgcggga aaccgctcaa gccacccaac tgacgcaggc tggccatgcg
      961 ctcgcgccga ccggtcagga tcttgtgcgg gtaggtttgg tggccgacat cccacaccag
     1021 ccggtcatgc ggggtgttga atacaaagtg cagtgccacc gtgagttcga ccgtgcccag
     1081 attggagctc aggtgcccgc cggtcttggc cacgctgtcg agcaaatagg cacgcaactc
     1141 ggtggccagc ggtgccagtt gcgcccgctc cagccgccgc aaatcgtcgg gtgcgtggat
     1201 ggtctgcaac agtgggtacg ggttattcat caacatatgc tttacttttt atagctactt
     1261 gcgcttatcc cataagggct agaggcactt ttaccaaata tctggaaagt gcctcaattg
     1321 gagcgttgta ccaccatgac cgccagtgcc tgtagcgcac gggtatcggt gaggccactg
     1381 cgcaccagtg ccgccagcgc ttgcgcgagc agatcctgcg caaaactgcc agccggcgcc
     1441 aaccccatca gcgacacata ggtgggtttg ttgttgtcag cgtccttgcc aaccgtcttg
     1501 cccaaggtgg ccgaatctgc ggtgacatcc agaatgtcgt ccaccacctg aaaggcaaga
     1561 ccaacggcgg caccaaattc ctgcagcgcc gcagtcgcca ttgcggtcgg ttggccgcag
     1621 gccagcccca tcatgacact ggcctgcaac agagcgccgg tcttcaggcg atgcatctcg
     1681 cgcaactgtg cctcggtgag ctgctgaccg acactggcca ggtcgatggc ctgcccgccc
     1741 gccatgccgg cgcaaccagc agcgcgtgac aaaagtcggc ataaatgggc ctgtcgctgc
     1801 accggc
//
LOCUS       DAOSLL010000724         1795 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_56743_length_1795_cov_3.618966, whole genome shotgun sequence.
ACCESSION   DAOSLL010000724 DAOSLL010000000
VERSION     DAOSLL010000724.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56743_length_1795_cov_3.618966"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="PLB25_21405"
     CDS             <1..643
                     /locus_tag="PLB25_21405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HOE44162.1"
                     /translation="CPAPTGPQSIVGCPFDPAQHWLLGTVGRMQAVKDQTTLAQAFVQ
                     AMASAPQLKANLRLVMIGDGPLRGQAQAILDQAGLGDLAWLPGERSDVADIMRGLHAF
                     ALPSLAEGISNTILEAMASALPVIATAVGGNADLVQQPDTGSIVPAADPSAMAQQIID
                     LASNPRLAHQMGLAGRQRVLDRFSMQSMVSTYQRVYNQQLSLSAALRAKRSSA"
     gene            763..>1795
                     /gene="asnB"
                     /locus_tag="PLB25_21410"
     CDS             763..>1795
                     /gene="asnB"
                     /locus_tag="PLB25_21410"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009856376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HOE44163.1"
                     /translation="MCGITGIFDTRGTTAVDRAVLQRMNDSQLHRGPDEGSLHLEPGI
                     GLGHRRLSIIDIATGQQPLFNEDGSVVVIFNGEIYNYQALIPELQALGHVFHTKSDTE
                     CIVHAWESWGADCVKRFRGMFAFALWDRNQQTLFLARDRLGVKPMYYAMLDNGTLLFG
                     SELKSILAHGDLRRDIDPLAVEEYFALGYVAEPRTIFKQASKLEPAHTLTIKRGQPLP
                     APVAYWDVKFTLDNPISEADAQAELVQRLQESVRLRMIAEVPLGAFLSGGVDSSAVVA
                     MMAGLSKDPVNTCSIGFDDPVFNESEFAQQVADRYHTNHRLEVVKSDDFDLIDTLARL
                     YDEPYADSSA"
BASE COUNT          373 a          564 c          502 g          356 t
ORIGIN      
        1 ttgcccagca cccaccggtc cccaaagcat tgtcggttgc ccatttgacc cggcccagca
       61 ttggctgcta ggcaccgtag gcagaatgca agcggtgaaa gatcaaacca cgctggccca
      121 ggcctttgtg caagcaatgg catcagcccc gcaactcaaa gccaatctgc gtctggtcat
      181 gattggcgat ggccctttgc gtggtcaggc ccaagccata ctggaccaag ctggcttggg
      241 tgatttggcc tggttgccgg gcgagcgcag cgacgtagcc gacatcatgc gcggtctgca
      301 cgccttcgcc ttgccttcgc tggccgaagg catttccaac accattttgg aggccatggc
      361 cagcgccctg cctgtgattg ctacagccgt gggcggcaac gccgaccttg tgcagcagcc
      421 agacaccggc agcattgtcc ctgcggcaga cccgagcgcc atggcgcagc agatcatcga
      481 cctggccagc aaccccaggc tggcccacca gatggggctg gcggggcgtc agcgcgtgct
      541 tgaccggttc agcatgcagt ccatggtgtc cacctatcag cgtgtctaca accagcaatt
      601 gagcctgtca gcggcactgc gagcgaagcg cagcagtgcg tgaacctgct ggctggattg
      661 ctttaccgcc ttcaccgagt gagaaccatt agtgcaatga cggatgaaat ggcgacatgc
      721 cgataaactc acttttccac caaaataaca acgggctaaa ccatgtgcgg tatcaccggt
      781 atttttgaca cgcgcggtac aaccgcagtt gaccgcgccg ttctgcagcg catgaatgac
      841 tcacaactgc accgtggccc cgacgaagga agcctgcacc ttgaacccgg cattggcctg
      901 ggccacaggc gcttgtccat catcgacatc gccaccggtc agcagccgct gtttaacgaa
      961 gacggttcag tggtggtgat cttcaacggt gaaatttaca actaccaggc cctcattcct
     1021 gagctgcaag cgctaggcca cgtgtttcac accaagagcg acactgaatg catcgtgcac
     1081 gcctgggagt cctggggtgc tgactgcgtc aagcgttttc gcggcatgtt tgcctttgcg
     1141 ctatgggacc gcaatcagca aaccttgttc ctcgcgcggg accgtctggg cgtcaaaccc
     1201 atgtactacg ccatgttgga caacggcacg ctgctatttg gctccgagct caaatccatt
     1261 ctggcgcacg gtgacctgcg ccgagacatt gacccgctgg cagtagaaga atactttgcc
     1321 ttgggctacg tggccgagcc acgcaccatc ttcaaacaag ccagcaagct cgaacccgct
     1381 cacaccctca cgatcaagcg cggccagccc ttgccagcgc cggttgctta ctgggatgtc
     1441 aagttcaccc tggacaaccc catctccgag gccgatgccc aagccgagct ggtgcagcgc
     1501 ctgcaagagt ctgtgcggct gcgcatgatt gctgaagtcc cgctgggggc attcctgtcc
     1561 ggcggcgtgg acagcagcgc tgtggtggcc atgatggcgg gcttgtccaa agacccggtc
     1621 aacacctgct ccataggatt tgacgacccg gtttttaacg aatccgagtt cgcccagcaa
     1681 gtggctgacc gctaccacac caaccaccgg cttgaagtgg tcaaaagtga tgactttgac
     1741 ctgatcgata ccctggcccg tctgtacgat gagccctacg ctgacagttc tgcga
//
LOCUS       DAOSLL010000725         1793 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_56827_length_1793_cov_5.348101, whole genome shotgun sequence.
ACCESSION   DAOSLL010000725 DAOSLL010000000
VERSION     DAOSLL010000725.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56827_length_1793_cov_5.348101"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="PLB25_21415"
     CDS             complement(<1..302)
                     /locus_tag="PLB25_21415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HOE44164.1"
                     /translation="MTLIQLGCIRGGRELFKDVDCTVQAGRWLYVVGPNGIGKTSLLR
                     MLCGLAPTESGEIRWNDLPIHTQRDRYLQDLCYLGHLNALQESMSVMENLAFSSALG"
     gene            complement(404..1147)
                     /gene="rquA"
                     /locus_tag="PLB25_21420"
     CDS             complement(404..1147)
                     /gene="rquA"
                     /locus_tag="PLB25_21420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodoquinone biosynthesis methyltransferase
                     RquA"
                     /protein_id="HOE44165.1"
                     /translation="MIFDKTAAVTSDTHLVARAAAHCPPVLVPDYLEKTYWWAYTHPN
                     AVRVFERQWLVNLILWGNFSRLRDLALQEMGHNLEGQVLQVACVYGNLTEHIVRRLGP
                     KAHLNVIDVAPVQIKNLHQKLHDKRQVSILQQDACNLSFHDASHDAVLVFFLLHEMPV
                     EVRRKTIAEALRVTKPGGKVIFVDYHKPVARSPFRYIMVPILSTLEPFAMDLWNNQIV
                     DWLPADGQYASIEKQTYFGGLYQKLVITR"
     gene            complement(1178..1786)
                     /gene="gstA"
                     /locus_tag="PLB25_21425"
     CDS             complement(1178..1786)
                     /gene="gstA"
                     /locus_tag="PLB25_21425"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019653612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HOE44166.1"
                     /translation="MKLYYSPGACSLAPHIALRESGLAFEAIAAPTKTHRLADGTDYY
                     TINPLGYVPWLVLDDGKQLHECAAILQFVADLVPDRQLAPANGSFARYKLQEWLNFIA
                     TELHKGFSPLFAPGMPDEAKAIAKTRVNGRLQWVDGEMADKSFLMGEHFTIADAYLFV
                     VTGWSKHVGLDISGLTNLSAFVDRIGARSAVQETLRAEGLTK"
BASE COUNT          409 a          493 c          498 g          393 t
ORIGIN      
        1 ccgagcgcag aggaaaatgc caggttctcc atcacgctca tcgattcctg tagcgcgttc
       61 aggtgtccga ggtaacacag gtcctgcaaa tacctgtcgc gctgggtgtg gatcggcagg
      121 tcattccatc gaatctcgcc ggactcggtc ggtgccaaac cgcacagcat gcgcaacagg
      181 ctggtcttgc caatgccatt ggggccgact acatacagcc agcgaccggc ttgtacggtg
      241 caatccacgt ccttgaacaa ctcacgccca cccctgatgc agccaagttg aatgagtgtc
      301 aatgacggaa agttgttcaa agggtcggca gttgcaacaa tgaatggcga attatcggac
      361 gaaccgcaac gcagctttgt gtgcgtcctg tcaaaagcca gggttagcgg gtgatcacca
      421 gcttttggta caagccacca aaataggtct gcttctcgat gctggcgtat tggccatctg
      481 caggtagcca gtcaacgatc tggttgttcc acaaatccat ggcaaagggt tccagcgtac
      541 tcaggattgg caccatgatg tatcgaaatg ggctgcgcgc caccggcttg tggtaatcca
      601 caaaaatcac cttgccaccc ggcttggtca cccgcaacgc ctcggcaatg gtcttccggc
      661 gaacctcaac cggcatttca tgcagcagga aaaacaccag cactgcgtca tggctggcat
      721 cgtgaaaact caaattgcag gcatcctgct gcaagatgct gacctggcgt ttgtcgtgca
      781 gcttttggtg gaggttcttg atttgaaccg gtgccacgtc aatgacgttc agatgagcct
      841 tggggcccag gcggcgcacg atgtgttcgg tcaggttgcc atagacacag gcaacttgca
      901 gcacctggcc ctcaaggttg tggcccattt cctgtagggc cagatcgcgc aggcgcgaaa
      961 aattgcccca taaaatcagg ttgaccagcc actggcgctc gaagacgcgc accgcattgg
     1021 ggtgtgtata ggcccaccaa taggtctttt ccaggtaatc cggaaccaaa acgggtgggc
     1081 aatgggcggc cgctctggca accaggtggg tgtcacttgt gacggcggcg gttttatcga
     1141 aaatcatgta gggtccctga gcaatttcaa tgccacttca tttagtcaga ccttcagcgc
     1201 gcaaagtctc ttgcacggct gaccgggcac cgatgcggtc aacaaaggcg ctcaggttcg
     1261 tcaaacctga aatatccagg ccgacatgtt tgctccagcc tgtcaccacg aatagatagg
     1321 catctgcaat ggtgaaatgc tcgcccatca gaaagctttt gtctgccatc tcaccatcca
     1381 cccattgcag gcgaccattg acacgggtct tggcgattgc ctttgcctca tcgggcatgc
     1441 ctggggcaaa caaaggtgaa aagcctttgt gcaattcagt ggcaatgaag ttgagccact
     1501 cctgcaactt gtagcgcgca aagctgccgt tggcgggtgc caactgccga tctggtacca
     1561 agtctgccac gaactgcagg atggccgcgc attcatgcaa ctgcttgccg tcgtcaagca
     1621 ccaaccaggg cacatagccc agcgggttga tggtgtagta atcagtgccg tcagccagtc
     1681 ggtgagtttt ggtcggtgcc gcaattgctt caaatgccag gccggattct ctcagggcaa
     1741 tgtggggtgc cagtgagcag gcacctggcg agtagtaaag cttcatttgg att
//
LOCUS       DAOSLL010000726         1787 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_57102_length_1787_cov_3.063510, whole genome shotgun sequence.
ACCESSION   DAOSLL010000726 DAOSLL010000000
VERSION     DAOSLL010000726.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57102_length_1787_cov_3.063510"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            190..1368
                     /gene="mraY"
                     /locus_tag="PLB25_21430"
     CDS             190..1368
                     /gene="mraY"
                     /locus_tag="PLB25_21430"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019574104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HOE44167.1"
                     /translation="MLIWLAQWLQNLSPDLGFLRVFQYQTFRAVMAAMTALLMGLAAG
                     PWVIRSLTSLKIGQPIRGYGMDTHLSKSGTPTMGGVLILLCITISTLLWFDWSNRFVW
                     IVLLVTLGFGAIGWSDDWRKVVNKDPEGMPSREKYLWQSLIGLVAALYLVFSISENTN
                     YRVLELFFTWVQSGFDLSLPPKAGLMVPFFKEVNYPLGVLGFVLLSYLVIVGSSNAVN
                     LTDGLDGLAIMPVVMVGACLGIFAYVTGNAVFARYLFLPHIAGAGELMVFCAAMGGAG
                     LAFLWFNAHPAQVFMGDVGALALGGALGTIAIIVRQEIVLAIMGGIFVAEALSVMLQV
                     TYFKYTRKKFGEGRRILKMAPLHHHFEKTGWKETQVVVRFWIITMLLCLVGLTTLKLR
                     "
     gene            1371..>1787
                     /locus_tag="PLB25_21435"
     CDS             1371..>1787
                     /locus_tag="PLB25_21435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HOE44168.1"
                     /translation="MNHLQHQHVLILGLGVSGLAMAHWCTRCGAQVTVADSRAAPPQL
                     AHLQRDLPGARFVAGALDASLLQGSQPVQAVFKSPGLAPPAIASLMIAASAMNIRSTG
                     ELGLFSEALAQLQAERGYRPVVLAITGTNGKTTVTAL"
BASE COUNT          291 a          497 c          588 g          411 t
ORIGIN      
        1 atgcctgatc tgcaggctgc ggtgctggcc gccctgccag gggtggccag tgtgctggtc
       61 aagggctcgc gcttcatgaa gatggagcgg gtgctgcagg tgttggtcaa cgacgacaag
      121 gcggtctttg atgctgcctg acttgttttt gaaaccggct tcagtgtcgg cagctcagcg
      181 gggtggcgca tgctgatctg gctcgcccaa tggctgcaaa acctgtcgcc cgatctcggc
      241 tttttgcggg ttttccagta ccagactttc cgcgccgtga tggctgccat gaccgcgctg
      301 ctgatggggc tggcggccgg gccgtgggtg attcgcagcc tgacttcgct gaagatcggg
      361 caaccgattc gcggctatgg catggacacc cacctgagca aaagcggcac gccgaccatg
      421 ggcggggtgc tgattttgct gtgcatcacg atctcgacct tgctctggtt tgactggagc
      481 aaccgttttg tctggatcgt gttgctggtg acgctgggtt ttggtgccat tggctggtcg
      541 gatgactggc gcaaggtggt taacaaagac cccgagggca tgccctcacg cgagaaatac
      601 ctgtggcagt cgctgattgg cctggtggcc gcgctgtacc tggtgttttc gatttctgaa
      661 aacaccaact accgggtgct ggaactgttt ttcacctggg tgcagtcggg tttcgacttg
      721 agcctgccgc ccaaggccgg gctgatggtg ccgtttttca aggaagtcaa ctacccgctg
      781 ggggtgctgg gttttgtgct gttgtcttat ctggtgattg tcggctccag caacgccgtc
      841 aacctgaccg acgggctcga cgggctggcc atcatgccgg tggtgatggt tggtgcctgc
      901 cttggcattt ttgcctatgt gacgggcaat gcggtgtttg cccgctacct ttttctgccg
      961 cacattgcgg gcgctggcga gttgatggtg ttttgcgccg ccatgggcgg ggccgggctg
     1021 gcatttttgt ggttcaacgc gcatccggcg caagtcttca tgggcgatgt cggcgcgctg
     1081 gcgctgggtg gtgcgctggg caccatcgcc atcatcgtgc gccaggaaat cgtgctggcc
     1141 atcatgggtg gaatttttgt cgcagaagcc ttgtccgtca tgctgcaggt gacctatttc
     1201 aaatacacca gaaagaagtt tggcgagggg cggcgcatcc tgaagatggc cccgctgcac
     1261 caccatttcg agaaaaccgg ctggaaagaa acgcaggtgg tggtgcgttt ctggatcatc
     1321 accatgctgt tatgcctggt gggactgacc accctgaagc tgcgctaacc atgaaccacc
     1381 tgcaacacca acatgtgctg atcctcgggc tgggtgtgtc tggcctggcc atggcgcact
     1441 ggtgcacccg ctgcggcgcg caggtgacag tggctgacag tcgcgccgcg ccgccgcaac
     1501 tggcccacct gcagcgcgac ctgcctggtg cgcgctttgt cgcaggtgcg cttgacgcaa
     1561 gcctgctgca gggcagccag ccagtgcagg cggtgttcaa aagcccgggc ctggcaccgc
     1621 ctgcaattgc ttcactgatg atagctgcca gcgcaatgaa cataaggtct acaggcgaat
     1681 tgggcttgtt ttctgaggcg ctggcgcagt tgcaggccga gcgcggctac cgcccggtgg
     1741 tgctggccat taccggcacc aatggcaaaa ccaccgtgac cgcactg
//
LOCUS       DAOSLL010000727         1773 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_57773_length_1773_cov_3.363213, whole genome shotgun sequence.
ACCESSION   DAOSLL010000727 DAOSLL010000000
VERSION     DAOSLL010000727.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1773)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1773
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57773_length_1773_cov_3.363213"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            331..1584
                     /gene="icd"
                     /locus_tag="PLB25_21440"
     CDS             331..1584
                     /gene="icd"
                     /locus_tag="PLB25_21440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013901513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HOE44169.1"
                     /translation="MYQHIKVPAEGQKITVNADMSLNMPDQPIIPFIEGDGTGLDITP
                     VMIKVVDAAVAKAYGGKKKIHWMEVYAGEKSTKVYGPDVWLPEETLAALREYVVSIKG
                     PLTTPVGGGIRSLNVALRQELDLYVCLRPIQYFEGVPSPVKEPHKTNMVIFRENSEDI
                     YAGIEFEAETPKAKKLIKFLQDEMGVTKIRFPETSGIGVKPVSREGTERLVRKAIQYA
                     IDNDKPSVTIVHKGNIMKYTEGGFRDWAYGLAQKEFGAELIDGGPWCKFKNPKSGKEI
                     TVKDSIADAFLQQILLRPAEYSVIATLNLNGDYISDALAAQVGGIGIAPGANLSDSVA
                     CFEATHGTAPKYAGKDYVNPGSEILSAEMMLRHMGWIEAADTIIKSMERSIKSKKVTY
                     DFARLMEGATQVSCSGFGQVMIDHM"
BASE COUNT          464 a          449 c          480 g          380 t
ORIGIN      
        1 gcctttttgg ctggcttgga agcagcgtca gctttcattt cggcttttac ttcggccttg
       61 gcagcagcct tggcaggtgc agcggcttca ggtgcagagg cagcggcgaa agcgccagca
      121 gcgaaaagac cagcaaccag agcagcgagg agtttgttca tttgtcagtt tccttggatt
      181 aacgttggta aaaaccttcg aacttttaga aggtgcagct acaacgccct caatgcagta
      241 tcggttgaca gcaaccagca atttaatcgg actaaaataa aattgttagt tctgacacga
      301 ttcccgaccc attcatggag atccaattca atgtatcaac acatcaaagt acctgctgaa
      361 ggccaaaaaa tcaccgtcaa cgcggacatg tccctgaata tgccagatca gccgatcatt
      421 ccgttcattg aaggcgatgg caccggcctc gatatcaccc cggtgatgat caaggtggtt
      481 gatgcagccg tggccaaggc atatggcggc aaaaagaaga ttcactggat ggaggtttat
      541 gccggcgaga aatccaccaa ggtctatggc cccgatgtat ggctgcccga agagactttg
      601 gctgcgttgc gcgagtatgt ggtgtccatc aaagggccgc tgaccacacc agtcggtggt
      661 ggcatccgct cgttgaacgt ggcgctgcgc caggaactcg acctttacgt gtgtctgcgc
      721 ccaattcagt attttgaagg cgtgcccagc ccggtcaagg aaccgcacaa aaccaacatg
      781 gtgatctttc gcgaaaactc ggaagacatc tatgctggca tcgaatttga agccgaaaca
      841 cccaaagcca aaaagctcat caagtttctg caggacgaaa tgggtgtcac caagatccgt
      901 ttccctgaaa cctcgggcat tggtgtcaag ccggtgtcgc gcgaaggcac cgagcgtctg
      961 gtacgcaagg cgattcaata cgcgatcgac aacgacaagc ccagcgtgac catcgttcac
     1021 aagggcaaca tcatgaaata caccgaaggt ggcttccgtg actgggccta tggtctggcg
     1081 caaaaagaat ttggtgccga gttgattgac ggtggcccat ggtgcaagtt caaaaacccg
     1141 aaatcgggca aggaaattac cgtcaaggac agcatcgctg atgccttttt acaacaaatt
     1201 ttgcttcgcc ctgctgagta cagtgtgatc gccaccttga atctgaatgg tgactacata
     1261 tctgacgcac tggcggcgca ggttggaggt attggcattg cgccgggcgc caacctgtca
     1321 gattcagtgg catgttttga agccacccat ggcacggcac ccaagtacgc gggcaaagac
     1381 tatgtgaacc ccggctcgga aatcctgtcc gcagagatga tgctgcgcca catgggttgg
     1441 attgaagcgg ccgacaccat catcaaatcg atggagcgct ccatcaagag caaaaaagtc
     1501 acttatgact ttgcccgcct gatggaagga gctacacagg taagctgctc gggctttgga
     1561 caagtcatga tcgaccacat gtagcaatcg acaaaggccg ccaaggacgg ccgaataaaa
     1621 cccctaagct gttgattcac ttaggggttt ttatcaccta cagacaagga tagacaggga
     1681 tagacccgct ggcagatggc ggcgagctat accttgagat tctgatgagt gggtcaaagg
     1741 tctggcgcta ctcatacggg ttcgagggaa agc
//
LOCUS       DAOSLL010000728         1766 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_58059_length_1766_cov_2.853302, whole genome shotgun sequence.
ACCESSION   DAOSLL010000728 DAOSLL010000000
VERSION     DAOSLL010000728.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58059_length_1766_cov_2.853302"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(207..635)
                     /locus_tag="PLB25_21445"
     CDS             complement(207..635)
                     /locus_tag="PLB25_21445"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HOE44170.1"
                     /translation="MFDQPIRNVMERRKFLTAPPEMTVSSAARLMASKNTGAILVVKS
                     KHLVGIFSERDAVFRVMAKGKDPETTLLSEVMTPSPVSMGPSQSFGHALLLMQEKGFR
                     HIPVVEDGHPIGIISSRNAMDPDLEEFVFEARRREHLRHS"
     gene            825..>1766
                     /locus_tag="PLB25_21450"
     CDS             825..>1766
                     /locus_tag="PLB25_21450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015013979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HOE44171.1"
                     /translation="MPAPSSKASSTPLALAVSDASSDIRTGTFVSFPDSPFELYQPYP
                     PAGDQPEAINQLVEGVENGEVFQTLLGVTGSGKTFTMANVIARLGKPAIVFAPNKTLA
                     AQLYSEFREFFPKNAVEYFVSYYDYYQPEAYVPQRDLFIEKDSAINEHIEQMRLSCTK
                     SLLERRDVVIVATVSAIYGIGQPEAYHKMVMTLRAGDKLGQRDMIAQLVRMQYNRNDI
                     DFSRGAFRVRGDTIDIFPAEHSEMAIRVELFDDEIESLQLFDPLTGRIRQKIPRFTVY
                     PSSHYVTPREQVLSAVEAIKLELAERIKEFVSQGKLVE"
BASE COUNT          370 a          491 c          522 g          383 t
ORIGIN      
        1 gaaatacacg ccaacgccca ggttcacctt gtttgggttg gtgtcggcgt tgtactgttc
       61 gttcaaaccc aaaatagggt cgcgcggggc catttcgaca gcggaaaaga gtgacatgaa
      121 agagtcctgt ggagttttgg gggaaacccg cattttactg agagttaagg gcagccagcc
      181 aaggcgccgg gctgcagcgc gtggcctcag gaatggcgca gatgttcgcg ccgccgggcc
      241 tcaaacacaa actcttccaa gtcggggtcc attgcattgc gtgacgaaat aatgccaatc
      301 gggtgcccat cttcaaccac ggggatgtgg cgaaagccct tctcctgcat gagtagcagc
      361 gcgtgcccaa acgactggct cggccccatg gacacaggcg agggcgtcat gacctcactg
      421 agcaaggtgg tttcggggtc cttgcctttg gccatgaccc gaaacacggc gtcgcgttcg
      481 gaaaaaatgc cgaccaggtg cttgcttttc accaccagaa tggcacccgt atttttgctg
      541 gccatcagcc gggcggcact gctgacagtc atttctggtg gtgcggtcag aaacttcctg
      601 cgctccatga catttcggat gggttgatcg aacatgctta tcctccagca gcaaatgcta
      661 gcgacactct ggcacatcac tttgatttgg gtcaacagga gatgatgcag ggcgcgaggg
      721 ggttcaggct ttgcgcacga ctcctgggca tgtggcagcg gcagcggcag cggagcttga
      781 cgctaccatg caggtctgta ccccactttc aattgactcc ccacatgcct gcacccagct
      841 caaaagccag ttcgacaccg cttgctctgg ctgtatctga cgccagctcc gacatccgca
      901 ccggcacctt tgtcagtttt cccgattcac cttttgagct gtaccagccg tatccgcccg
      961 cgggtgacca gcctgaggcc atcaatcagc tggtggaggg ggtggaaaac ggcgaggtgt
     1021 tccagacttt gctgggcgtc accggctccg gcaagacctt caccatggcc aatgtgattg
     1081 cccgccttgg caagccggcg atcgtctttg cacccaacaa gacgctggcg gcgcagttgt
     1141 attcagagtt tcgcgagttt tttccgaaga acgcggtgga gtatttcgtc agttactacg
     1201 attactacca gcccgaggcc tatgtgccgc agcgtgattt gtttattgaa aaagactcgg
     1261 cgatcaatga gcatattgag cagatgcgcc tatcgtgcac caagagcctg ctggagcgcc
     1321 gtgatgtggt gattgtggcc accgtctcgg cgatctatgg cattggccaa cccgaggctt
     1381 atcacaagat ggtgatgacg ctgcgcgcgg gtgacaaact gggccagcgc gacatgattg
     1441 cgcaactggt gcgcatgcag tacaaccgca acgacatcga cttttcacgc ggcgcgttcc
     1501 gtgtgcgggg cgacaccatc gacatatttc cagccgagca ttccgagatg gcaattcggg
     1561 ttgagctgtt tgatgacgag attgaaagtc tgcaactgtt tgacccgctg accgggcgca
     1621 tccggcagaa gatcccgcgc ttcacggtct atcccagtag ccactatgtg actccgcgcg
     1681 agcaggttct gtcggcggtg gaagcgatca agttggagct ggcagagcgc atcaaggagt
     1741 tcgtgtccca gggcaagctg gtggaa
//
LOCUS       DAOSLL010000729         1760 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_58300_length_1760_cov_4.650440, whole genome shotgun sequence.
ACCESSION   DAOSLL010000729 DAOSLL010000000
VERSION     DAOSLL010000729.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58300_length_1760_cov_4.650440"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="PLB25_21455"
     CDS             complement(<1..257)
                     /locus_tag="PLB25_21455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HOE44172.1"
                     /translation="MTTAVFSLAAGVPRHRTRRVLPGFHLTLGYTLFYLGLIVLMPLS
                     ALLFKTFTLTWDQFWAAVTSARVLASYRLTFGASFLAALVNV"
     gene            complement(336..614)
                     /locus_tag="PLB25_21460"
     CDS             complement(336..614)
                     /locus_tag="PLB25_21460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HOE44173.1"
                     /translation="MALAIAVIALQATITVAYQIPAEGIMQATEAHSIWILQLTVGFH
                     LQVLMRIYFFGFDGFHPLHGSATHPIEPLATRLLSLLIPRPPGPHAGS"
     gene            complement(841..>1760)
                     /locus_tag="PLB25_21465"
     CDS             complement(841..>1760)
                     /locus_tag="PLB25_21465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HOE44174.1"
                     /translation="CPMCGAAQDPQRRYFPKPGQEQEAKGHRFVGIDWVCAYCESPNS
                     AAAAFCVNCGGARDGTKEASLVQDKTASVMPASPVLPVAAAAPSGFPWGKAALALLLV
                     VCSVLAWLFLSKHDESVQIAEKSWSRSIDVERFSAVRSADWCDQMPPDAYLVSRSREQ
                     RSTRQVEDGQDCVEVRADRGDGTFSKSSQCSPRYRSEPVFDLKCSFRVNRWQLARTDK
                     LVGTASLTPAWPAPALGNTLLGGNSLGAERLGTRRESYRVRLQSAQGKEWHCDVDAGI
                     WSGLAEKQPMTLKIRNTGGADCASLAASR"
BASE COUNT          365 a          533 c          534 g          328 t
ORIGIN      
        1 acattgacca gcgccgccag gaacgatgcg ccaaaggtca gccggtacga ggccagcacg
       61 cgcgcactgg tgactgccgc ccaaaactgg tcccaggtga gtgtgaaggt cttgaacaac
      121 agggcagaca acggcatcag cacaatcaga ccaaggtaga acagcgtgta gcccagcgtc
      181 agatgaaaac ccggcaacac gcgccgggtc cggtgtctgg gcacacccgc tgccagtgag
      241 aaaacagccg tcgtcattga aactggatgc ctcggttgga cgcgcgcggg tgggtcgctg
      301 gcggcgcgcc tgggccgacg cgatagcgac gcgggttaac tgcccgcatg tggtcctggc
      361 ggacgaggga tgagcagcga caacaggcgc gttgccagcg gttcaatcgg gtgcgttgcg
      421 ctgccatgca atgggtgaaa gccatcgaag ccaaaaaagt aaatcctcat aaggacttgc
      481 agatgaaaac caacggtcaa ctgaagaatc cagattgaat gtgcttcggt tgcctgcatt
      541 atgccttcag cgggtatttg atatgctact gtaatagtag cttgtaacgc aataacagca
      601 atggctagag ccacttttta cccttatttc agggtgtttc gtcagggcct tgatgacagg
      661 tgtcaaacag ctctggacga tgccaggcgc agccgacagc ggctgattca aagcttggcc
      721 gcagcacctg cgagaaagtg cgctggtaaa ggcgctgcat tggcacggcg acggtctgtg
      781 tcgcaccaac ccaagtccct gtagctcaaa gtaaagcgtt gccgcgaggc cggatggcaa
      841 ttacctgctg gcagccaggc tggcgcaatc agcgccaccg gtgttgcgga ttttcaaggt
      901 catgggctgc ttttcagcca gccccgacca gatgccggca tcaacatcgc aatgccattc
      961 cttgccctgc gccgactgca accggacccg gtaactttct cgccgcgtgc cgagccgctc
     1021 ggcacccagg ctgttgccgc ccagcagggt attgcccagc gctggtgcgg gccaggccgg
     1081 tgtcaaactg gcagtgccga ccaatttgtc ggttcgcgcg agctgccagc ggttgacgcg
     1141 aaaactgcac ttgagatcga aaaccggttc gctgcggtag cgggggctgc attggctgct
     1201 tttgctgaaa gtgccgtcgc cccggtcggc gcgcacttcc acgcagtcct ggccgtcttc
     1261 gacctggcgc gtgctgcgct gctcgcgact gcggctgacc aggtaggcat cgggcggcat
     1321 ttgatcgcac cagtcggcgc ttctgacggc gctgaaacgc tccacgtcga tgctgcgact
     1381 ccaggacttc tcggcaatct gcacgctttc gtcatgtttg ctcaaaaaca accaggccag
     1441 caccgaacag accaccagca gcaacgccag cgccgccttg ccccatggaa atcctgacgg
     1501 cgctgctgct gccaccggca gcaccgggct ggctggcatc acgctggcag tcttgtcctg
     1561 caccaggctt gcctcttttg tgccatccct ggccccgccg caattgacgc aaaaggcagc
     1621 agcagcagag ttgggcgatt cgcagtaggc acaaacccag tcgatgccga caaagcgatg
     1681 gcctttggcc tcttgctcct gaccaggctt ggggaaatag cgccgctgcg ggtcttgggc
     1741 ggcaccgcac atcgggcaat
//
LOCUS       DAOSLL010000730         1736 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_59489_length_1736_cov_2.518739, whole genome shotgun sequence.
ACCESSION   DAOSLL010000730 DAOSLL010000000
VERSION     DAOSLL010000730.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1736
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59489_length_1736_cov_2.518739"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            19..633
                     /locus_tag="PLB25_21470"
     CDS             19..633
                     /locus_tag="PLB25_21470"
                     /inference="COORDINATES: protein motif:HMM:NF016246.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOE44175.1"
                     /translation="METTIYTVGHSNHSIERFLSILQAAEVTAIADVRSQPFSRYNPQ
                     FNRGALREVLRAHGIAYAFVGKELGARPGDPGCYERGQVQYGRLAATVLFKAGLDRVM
                     AASHKYRIALMCAEKEPLVCHRTLLVGQALKGRGVNLVHILANGDIEPDDDAMRRLLD
                     MTGVPRLDLFRTPDELLQEAMARQMQGLTYSDDKQTGDINGDVE"
     gene            630..1067
                     /locus_tag="PLB25_21475"
     CDS             630..1067
                     /locus_tag="PLB25_21475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009459601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HOE44176.1"
                     /translation="MKLFTIGFTKKSAQQFFENLRASGARRVVDVRLNNVSQLAGFAK
                     KNDLAYFLKIICDMDYLHLPELAPTQEMLDQYKKFGGDWATYENRFLDLLEKRQIEQS
                     LPRAMLSDGCLLCSEDKPHCCHRRLVAEYLKRHWGDIQITHLG"
     gene            1297..>1736
                     /locus_tag="PLB25_21480"
     CDS             1297..>1736
                     /locus_tag="PLB25_21480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011800850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HOE44177.1"
                     /translation="MKIAIVGSGISGLAVAHRLKGRADLTLFETGSYFGGHTHTVDIT
                     LDTPKGPVTHGVDTGFLVFNERTYPNLIQLLAELDVATTKSDMSFSVQVPGGRVGGPL
                     EWSGTSLSTVFAQRGNLLNPRFWRMLRDLLRFNSFTNQLASSGD"
BASE COUNT          388 a          457 c          503 g          388 t
ORIGIN      
        1 tgaacgatgg acatgagcgt ggagacgacg atctacaccg ttgggcattc aaatcactcc
       61 atcgagcgtt tcctgagcat tctccaggcc gccgaggtga ctgcaattgc agatgttcgg
      121 tcacaaccgt tcagcagata caacccccaa ttcaacaggg gcgcactccg ggaggtgttg
      181 cgcgcccacg gcattgccta cgcgtttgtt ggaaaagagt tgggcgcgcg cccgggtgac
      241 ccaggctgct acgagcgtgg gcaagtgcaa tacggacggc tggctgcgac agttttgttc
      301 aaggcgggtc tggatcgtgt gatggcggcc tcgcacaagt acaggattgc attgatgtgt
      361 gctgagaagg agccactggt ttgtcaccgc accttgctgg tgggccaagc cctcaagggc
      421 cgaggcgtga accttgtgca tattcttgcc aatggtgaca ttgagcctga cgatgacgcg
      481 atgcgtcgat tgcttgacat gaccggcgtg ccacgcttgg acctcttccg aacgccggat
      541 gagttgctgc aagaggccat ggccagacag atgcaaggct tgacgtacag cgatgacaag
      601 cagaccgggg acatcaatgg ggacgtcgaa tgaagttatt caccatcgga ttcaccaaga
      661 aaagcgcgca gcagttcttt gagaacttgc gggcgtcagg tgccaggcgt gtggtggacg
      721 tccggctgaa caatgtttcg caacttgccg ggtttgcaaa gaagaatgac ctggcctatt
      781 tcctcaaaat tatctgtgac atggactacc tgcacttgcc ggaactggct ccaacgcagg
      841 agatgctgga tcagtacaag aagttcggtg gtgattgggc aacatatgaa aatcgatttc
      901 ttgacttgtt ggaaaaacgg caaattgaac agtccttgcc cagggccatg ttgtcggatg
      961 gctgccttct gtgcagtgaa gacaagccac actgctgcca ccgccgtctg gtggctgagt
     1021 atctgaagcg gcattgggga gatatccaaa tcacgcatct gggttgacgg caatccgcgg
     1081 ttcattgcca tcatgcttgg cctgttcagt ctgtcccagg caggcgagcc gacgctcaag
     1141 cttggaagag cgcagctaca tcgtctggcc ggcacgcact catccatgtc gcccgcatgg
     1201 aaatagcaaa tggcgcggcg tgcataaaac caatcaatgt gctaaattac caatcagtcg
     1261 caaaaatacg aaactgtttt aaactctcct tttgtcatga aaatcgccat tgtcggctcc
     1321 ggtatttctg gtttggctgt tgcccatcgg ctcaaaggtc gtgccgatct caccttgttt
     1381 gagaccggga gttatttcgg tggacacacc cataccgtcg acatcacgct ggacacccca
     1441 aaaggcccgg ttacccacgg tgtggatacg gggttcctgg tgttcaacga gcgcacctac
     1501 cccaatctga ttcagttgct ggcagagctg gatgtcgcca ctacaaagtc cgacatgtct
     1561 ttctcggtgc aagtgccagg cgggcgtgtc ggtgggccac tggagtggag tggtacatca
     1621 ttgagcacgg tgttcgccca acggggcaac ctgctcaacc cgcgtttctg gcgcatgctg
     1681 cgcgacttgt tgcgcttcaa tagctttacc aaccagctgg ccagcagcgg agacga
//
LOCUS       DAOSLL010000731         1720 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_60273_length_1720_cov_4.777177, whole genome shotgun sequence.
ACCESSION   DAOSLL010000731 DAOSLL010000000
VERSION     DAOSLL010000731.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60273_length_1720_cov_4.777177"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..343
                     /locus_tag="PLB25_21485"
     CDS             53..343
                     /locus_tag="PLB25_21485"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HOE44178.1"
                     /translation="MRLRELFSIPGTSVKGENIVVLKYSGQKAGLVVDTLLGEFQTVI
                     KPLGQMFAQSKCISGSTILGSGDVALILDVPSLVRQAMAKGGQMPQEHAVMA"
     gene            394..>1720
                     /locus_tag="PLB25_21490"
     CDS             394..>1720
                     /locus_tag="PLB25_21490"
                     /inference="COORDINATES: protein motif:HMM:NF024140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCP four helix bundle domain-containing protein"
                     /protein_id="HOE44179.1"
                     /translation="MFANMKIGVRLAIGFALTLVLLIVVSVIGVARIAALQTEVEGLV
                     KDKFPKTVVANDAIDAINTIARRLRNAYIFPAAEGQKELDAIPEQRKVISDRLEKLEK
                     TITSDAGKAVLKKVLDARASYVAQQDKYIDVLKANKREEAVSLLQGDLRKAQNDYMSS
                     IVALIDFQTELVTKAGKDAEDLASSSERLLIILAVVAAVLSIVAGWLITRSITGPTRL
                     LMEGADKMAAGNFEFKLDIHNKDEIGALAKSVGAMQTAVQAMIADANMLVKAAVEGKL
                     ETRADASKHQGDFNKIVAGVNQTLDAVIGPLNVAAGYVDRISKGDIPPKITDTYNGDF
                     NTLKNNLNQAISSVNALVADANMLAKAAVEGKLETRADASKHQGDFQKIVAGVNNTLD
                     AVIKPLNVAAGYVDRISKGDIPPKITDSYNGDFNTLKNNLNQAISSVNAL"
BASE COUNT          413 a          441 c          499 g          367 t
ORIGIN      
        1 gtaaaacccc tactgtcgcc ccggcaaagc cgggggatta cctgttttat ttatccggct
       61 gcgtgaactg ttttcgattc cgggcacgtc ggtcaaaggt gaaaacattg tggtgctcaa
      121 gtattccggg caaaaagccg ggttggtggt ggacaccttg ctgggcgagt ttcagacagt
      181 gatcaagccg ctggggcaga tgtttgccca aagcaaatgc atcagcggct cgaccatttt
      241 gggcagtggc gatgtggcct tgattctgga tgtgccatca ctggtgcgcc aggccatggc
      301 caaaggtggt cagatgccgc aagagcatgc agtgatggcg tgagtttgac gatgcgttgg
      361 attaatcagg tcttttgata aaaggggtac gacatgtttg caaatatgaa gattggcgtc
      421 aggttggcca ttggctttgc gctgactttg gtgttgctga tcgtggtatc ggtcatcggg
      481 gtggcccgaa tcgcggcatt gcagaccgag gtcgagggtc tggtgaaaga caagtttccc
      541 aagaccgtgg tggccaatga tgctattgat gccatcaaca cgattgcccg gcgacttcgc
      601 aatgcctaca tttttccggc cgcagaaggg caaaaggagt tggatgccat tcccgagcaa
      661 cgcaaggtca tctcggatcg tcttgaaaaa ctcgagaaaa ccatcaccag cgatgctggc
      721 aaggcggttt tgaaaaaggt gctggatgcc cgcgcctcct atgtggcgca gcaggacaaa
      781 tacatcgacg tgctcaaggc caacaagcgt gaagaggctg tgtccttgtt gcaaggtgat
      841 ttgcgcaaag cgcagaacga ctacatgtcg agcatcgttg ccctgataga ttttcagact
      901 gaattggtga caaaggctgg caaagatgcc gaagacctgg ccagttcgtc cgagcgcctt
      961 ctgatcattc tggcagtggt agcagccgtc ctcagcatcg tggcgggctg gttgatcacc
     1021 cgctcaatca ctggtccgac ccgtctgctg atggaaggtg ccgacaagat ggccgcaggc
     1081 aattttgagt tcaagctcga tatccacaac aaggacgaaa ttggcgcgct ggccaagtca
     1141 gtgggcgcca tgcagactgc tgtgcaggcc atgattgccg atgccaacat gctggtcaag
     1201 gcagccgtgg aaggcaagct cgaaacacgc gccgatgctt ccaaacacca gggcgacttc
     1261 aacaagatcg tggccggtgt caaccagacg ctcgatgccg tgattggccc gctgaatgtg
     1321 gccgccggtt atgtggaccg catctccaag ggtgacatcc caccgaagat taccgacact
     1381 tacaacggtg atttcaacac gctcaagaac aacctgaacc aggccatcag ctcggtcaac
     1441 gcattggtgg cagatgccaa catgctggcc aaggcagccg tggaaggcaa gcttgaaacc
     1501 cgcgccgatg cttccaagca tcagggtgac ttccagaaga ttgtggccgg tgtcaacaac
     1561 acgctagatg cagtgatcaa accactgaat gtcgccgccg gttatgttga tcgtatttcc
     1621 aagggcgaca ttccgccgaa gatcaccgat agctacaacg gtgacttcaa cacgctcaag
     1681 aacaacctga accaggccat cagctcggtc aacgcattgg
//
LOCUS       DAOSLL010000732         1585 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_67781_length_1585_cov_3.614379, whole genome shotgun sequence.
ACCESSION   DAOSLL010000732 DAOSLL010000000
VERSION     DAOSLL010000732.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_67781_length_1585_cov_3.614379"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(169..711)
                     /gene="msrA"
                     /locus_tag="PLB25_21495"
     CDS             complement(169..711)
                     /gene="msrA"
                     /locus_tag="PLB25_21495"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HOE44180.1"
                     /translation="MTVQTQTITLGGGCFWCTEAVFVNVKGVLDVESGYCNGHVRQPS
                     YEQVCTGNTGHNEVVKLVFDPGQISLREVLEIFFVIHDPTSRNAQGNDHGTQYRSGIY
                     FTTPEQQAVALEVIGDMTDSGSHTRPIVTEVLGLSNYWAAEDYHQDFFARNPHQGYCM
                     AMAAPKVAKFRKTFARLQKD"
BASE COUNT          357 a          425 c          448 g          355 t
ORIGIN      
        1 cgttgagagc aataacgcac gaggcgacaa atggttgtca tgaattatga aaagacgctg
       61 aaagctatgg cgagctagcg ttcaagcacc aaaaatgttg tgtgccaatt gaccatcgga
      121 ccaaatcctt cgcccccagg tcactgagag tcattgatag ggcagcgatc agtccttttg
      181 cagccgcgca aaggtcttgc gaaacttggc aaccttgggt gcagccatgg ccatgcagta
      241 gccctgatgc gggttgcgcg caaagaagtc ctggtggtag tcctcggccg cccagtagtt
      301 gctcaggccc agcacttcgg tcacgatcgg gcgtgtgtgg ctgccgctgt ctgtcatgtc
      361 gccgatgacc tccagcgcca cagcttgctg ctctggcgtt gtgaagtaaa tgccgctgcg
      421 gtattgggtg ccgtggtcgt tgccctgggc attgcggctg gtcgggtcgt gaatgacaaa
      481 aaagatctcc agaacttcgc gcaggctgat ctggccaggg tcaaacacca gcttcaccac
      541 ttcgttgtga ccggtgttgc cggtgcatac ctgctcgtag ctgggctggc gcacatggcc
      601 attgcagtaa ccagattcga catcaagcac acctttgacg ttgacaaaaa ccgcctcggt
      661 gcaccagaag cagccacctc ccaaggtgat ggtttgtgtt tgaacagtca tgggatgggt
      721 tccttggggt tgagatggat gtgcgacgcg gcaggtcgat ggtagcggga atgaatgacc
      781 ctactcacca gcagctcagc gtttccaaac tactgcgttg aggccgtcaa gcccccagaa
      841 tgatatctat ttgagagctg tcaacgcata gatgacaagc gctagagggc aaaaaccttc
      901 aaaacgtgcc agcaacattg cctggccgaa aggcacctgc agcatgcccg gcgcagtggc
      961 ggcctggcaa catcagatat cggcttccca ctggcgaagc agattcaaac ttgtatcttc
     1021 ggatttctga attggatagt ggtggtaggt ggctttggcg cttgtgggca acggatcacg
     1081 cggtgcgcaa cgccccaggc ggtttcggcg gcaggtgatc cggtttgtcg gcaggcaaat
     1141 tgtccacaaa tccacagcct gtttgcagtg aagatcgaat cctgctgtgc agtccgtttc
     1201 aatccagatt actcagttgc ccccctgttt tcctctgtaa gtcctcatga atattgacct
     1261 tttcagcttc gatggagttc accgattggg ccacagcgct gcgcacccga ttgaaccgct
     1321 ggcaacgcgc ctgagagcgt tacgcctccc agcgcaggat ttgagcgtcg tcgtcgcgcc
     1381 tacccagccg cgccgtcagc ggaccacttg ggcgcgtcct tctgagtaac ccaggttcaa
     1441 ttgatccagc cctgtaagct ggcaacagac caaagcaatg gatgcttgtc aaggaggagt
     1501 ctggaccgtt gaaacgtcaa atctgttgct aacggcatgt tgcaaaagcc aataagccat
     1561 cattggaaaa taattctgca ggaaa
//
LOCUS       DAOSLL010000733         1521 bp    DNA     linear   ENV 07-JUN-2024
DEFINITION  MAG TPA_asm: Rhodoferax sp. isolate AMR_MDS_1624
            NODE_71963_length_1521_cov_5.529332, whole genome shotgun sequence.
ACCESSION   DAOSLL010000733 DAOSLL010000000
VERSION     DAOSLL010000733.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32863153
            Sequence Read Archive: SRR4236665
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Rhodoferax sp. (wastewater metagenome)
  ORGANISM  Rhodoferax sp.
            Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
            Comamonadaceae; Rhodoferax.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-MAR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 02:13:38
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 4,302
            CDSs (total)                      :: 4,278
            Genes (coding)                    :: 4,174
            CDSs (with protein)               :: 4,174
            Genes (RNA)                       :: 24
            tRNAs                             :: 22
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 104
            CDSs (without protein)            :: 104
            Pseudo Genes (ambiguous residues) :: 0 of 104
            Pseudo Genes (frameshifted)       :: 30 of 104
            Pseudo Genes (incomplete)         :: 82 of 104
            Pseudo Genes (internal stop)      :: 9 of 104
            Pseudo Genes (multiple problems)  :: 16 of 104
            CRISPR Arrays                     :: 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Rhodoferax sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71963_length_1521_cov_5.529332"
                     /isolate="AMR_MDS_1624"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:50421"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="43.77 N 142.37 E"
                     /collection_date="2015-04-20"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..376)
                     /gene="secG"
                     /locus_tag="PLB25_21500"
     CDS             complement(17..376)
                     /gene="secG"
                     /locus_tag="PLB25_21500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013593733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HOE44181.1"
                     /translation="MGVVSTILLAVQMISALAMVGLILVQHGKGADMGAAFGSGGSGS
                     LFGASGSANFLSRTTAVLATVFFVATLLLAYFGSVRPAASAGSGSVLENAALVAPPPA
                     AVAPVLPASGAAQIPAK"
     gene            complement(415..1164)
                     /gene="tpiA"
                     /locus_tag="PLB25_21505"
     CDS             complement(415..1164)
                     /gene="tpiA"
                     /locus_tag="PLB25_21505"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020425084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HOE44182.1"
                     /translation="MINMKKLIAGNWKMNGSLAANEKLLKDILAGLGQAKCQVAVGVP
                     AVYLAQCQALLTGTAIDLGSQDISTQESGAYTGEISGAMLKEFGVRYAIVGHSERRQY
                     HGETDELVALKAQRALGFGITPIVCIGETLAEREAGKTDEVVKRQLAAVIHTNGHCIS
                     EIVVAYEPVWAIGTGKTATPEMAQQVHAVLRAQLKAASEQADRIKLLYGGSMNAANAA
                     SLLAQPDIDGGLIGGAALKAPDFLTIIAAAA"
     gene            complement(1161..>1521)
                     /locus_tag="PLB25_21510"
     CDS             complement(1161..>1521)
                     /locus_tag="PLB25_21510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HOE44183.1"
                     /translation="GVDVVLDMVAGSYVAKEVQCLKDDGRLVIISIQGGVKSDFNAGL
                     VLRRRLTITGSTLRPRPIAFKAAIAQACLKEVWPALAAKRIKPVIYGTFAAADVALAH
                     TLMESSQHVGKIVLTWR"
BASE COUNT          338 a          497 c          383 g          303 t
ORIGIN      
        1 cgaatctcaa gtgcaattat ttggccggaa tctgcgctgc gccagaggcc ggcagcaccg
       61 gtgcaactgc cgcaggagga ggtgccacca aagcagcatt ttccagcaca ctaccgctgc
      121 cagcgctggc agccgggcga accgaaccaa aatatgccag caacaaggtc gcaacaaaaa
      181 acaccgttgc cagcactgcc gtggttcgtg acaggaagtt ggcgctgcca ctcgcgccga
      241 acaggctacc cgaacctccg ctgccgaaag ctgcccccat gtcggcccct ttgccatgct
      301 gaaccaggat cagcccaacc atggccagcg cagaaatcat ctgcacagcc agcaagattg
      361 tcgaaacaac acccatcaac tgctcctaat tcaatagcta tcggcgcttt attctcaagc
      421 ggctgcggca ataatcgtca aaaagtccgg ggcctttaag gcagccccgc cgatcaagcc
      481 accgtcaatg tccggttgtg ccagcaacga tgccgcattg gctgcgttca tgctgccacc
      541 gtacaacagt ttgatgcggt ctgcctgctc ggaagccgcc ttgagttgcg cgcgcaacac
      601 cgcgtgcacc tgctgcgcca tctcgggtgt tgcagttttg ccggtgccaa ttgcccacac
      661 cggctcatac gccaccacaa tttcgctgat gcagtgaccg ttggtgtgaa tcacggcggc
      721 cagttgccgc ttgacaacct cgtcggtttt gccggcttcg cgctctgcca gggtctcgcc
      781 gatacagacg atcggggtga taccgaaacc caaggcgcgc tgtgccttga gtgccaccaa
      841 ctcatcggtt tcaccatggt attgacgacg ctccgaatgc ccgacgatgg cgtaacgcac
      901 gccaaactcc ttgagcatgg cacctgaaat ttcaccggtg taggccccgc tctcctgtgt
      961 agaaatatcc tgcgacccaa gatcaatagc agtaccggtc agcagcgcct ggcactgcgc
     1021 caggtaaacc gcaggcacgc ccaccgcgac ctggcacttc gcctgcccaa ggcccgcgag
     1081 gatgtctttg agaagttttt cgttggcagc caggctgccg ttcattttcc agttacccgc
     1141 aatcagtttt ttcatgttga tcatctccaa gttaaaacaa ttttccccac atgctggctc
     1201 gactccatca aggtgtgtgc caacgccacg tctgcagccg caaatgtgcc ataaataacc
     1261 ggcttgatgc gctttgccgc caatgccggc cagacttctt taaggcaagc ctgcgcgata
     1321 gccgccttga acgcgatcgg gcgcggtcgc agggttgaac cggtaatggt caacctgcgg
     1381 cgcaacacca acccggcatt gaagtcgctc ttgacaccgc cctgaatcga aatgatcacc
     1441 aggcgaccgt cgtccttcag acactgaact tccttggcca catagctgcc agccaccatg
     1501 tccaatacca cgtccacacc a
//
